LOCUS       DAMKPG010000001       156362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMKPG010000001 DAMKPG010000000
VERSION     DAMKPG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 156362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 156362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..156362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.001"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(94..1173)
                     /locus_tag="PC621_000001"
     CDS             complement(94..1173)
                     /locus_tag="PC621_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF4107193.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            complement(1329..2678)
                     /locus_tag="PC621_000002"
     CDS             complement(1329..2678)
                     /locus_tag="PC621_000002"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF4107194.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            complement(2943..4571)
                     /locus_tag="PC621_000003"
     CDS             complement(2943..4571)
                     /locus_tag="PC621_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF4107195.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            complement(4695..6299)
                     /locus_tag="PC621_000004"
     CDS             complement(4695..6299)
                     /locus_tag="PC621_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107196.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            complement(6347..6664)
                     /locus_tag="PC621_000005"
     CDS             complement(6347..6664)
                     /locus_tag="PC621_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107197.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            complement(6669..7034)
                     /locus_tag="PC621_000006"
     CDS             complement(6669..7034)
                     /locus_tag="PC621_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107198.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            complement(7115..8152)
                     /locus_tag="PC621_000007"
     CDS             complement(7115..8152)
                     /locus_tag="PC621_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF4107199.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            complement(8405..8989)
                     /locus_tag="PC621_000008"
     CDS             complement(8405..8989)
                     /locus_tag="PC621_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107200.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            complement(9004..9447)
                     /locus_tag="PC621_000009"
     CDS             complement(9004..9447)
                     /locus_tag="PC621_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107201.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            complement(9448..10320)
                     /locus_tag="PC621_000010"
     CDS             complement(9448..10320)
                     /locus_tag="PC621_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF4107202.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            complement(10373..10732)
                     /locus_tag="PC621_000011"
     CDS             complement(10373..10732)
                     /locus_tag="PC621_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF4107203.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(10786..11871)
                     /locus_tag="PC621_000012"
     CDS             complement(10786..11871)
                     /locus_tag="PC621_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF4107204.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(12042..13421)
                     /locus_tag="PC621_000013"
     CDS             complement(12042..13421)
                     /locus_tag="PC621_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF4107205.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(13414..14094)
                     /locus_tag="PC621_000014"
     CDS             complement(13414..14094)
                     /locus_tag="PC621_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF4107206.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(14486..15565)
                     /locus_tag="PC621_000015"
     CDS             complement(14486..15565)
                     /locus_tag="PC621_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4107207.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      15853..15968
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16143..17513
                     /locus_tag="PC621_000016"
     CDS             16143..17513
                     /locus_tag="PC621_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4107208.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(17778..18389)
                     /locus_tag="PC621_000017"
     CDS             complement(17778..18389)
                     /locus_tag="PC621_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF4107209.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(18379..20742)
                     /gene="lon"
                     /locus_tag="PC621_000018"
     CDS             complement(18379..20742)
                     /gene="lon"
                     /locus_tag="PC621_000018"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF4107210.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            20922..21434
                     /locus_tag="PC621_000019"
     CDS             20922..21434
                     /locus_tag="PC621_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF4107211.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            21422..21949
                     /locus_tag="PC621_000020"
     CDS             21422..21949
                     /locus_tag="PC621_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF4107212.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(22020..23270)
                     /gene="clpX"
                     /locus_tag="PC621_000021"
     CDS             complement(22020..23270)
                     /gene="clpX"
                     /locus_tag="PC621_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF4107213.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(23289..23873)
                     /gene="clpP"
                     /locus_tag="PC621_000022"
     CDS             complement(23289..23873)
                     /gene="clpP"
                     /locus_tag="PC621_000022"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF4107214.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(24022..25308)
                     /locus_tag="PC621_000023"
     CDS             complement(24022..25308)
                     /locus_tag="PC621_000023"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF4107215.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(25502..25972)
                     /locus_tag="PC621_000024"
     CDS             complement(25502..25972)
                     /locus_tag="PC621_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4107216.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(25972..27321)
                     /gene="rph"
                     /locus_tag="PC621_000025"
     CDS             complement(25972..27321)
                     /gene="rph"
                     /locus_tag="PC621_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF4107217.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(27481..29505)
                     /gene="ligA"
                     /locus_tag="PC621_000026"
     CDS             complement(27481..29505)
                     /gene="ligA"
                     /locus_tag="PC621_000026"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF4107218.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(29691..30629)
                     /locus_tag="PC621_000027"
     CDS             complement(29691..30629)
                     /locus_tag="PC621_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF4107219.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(30731..31153)
                     /locus_tag="PC621_000028"
     CDS             complement(30731..31153)
                     /locus_tag="PC621_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF4107220.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            31341..32624
                     /locus_tag="PC621_000029"
     CDS             31341..32624
                     /locus_tag="PC621_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF4107221.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(32702..33217)
                     /locus_tag="PC621_000030"
     CDS             complement(32702..33217)
                     /locus_tag="PC621_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF4107222.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(33217..34593)
                     /locus_tag="PC621_000031"
     CDS             complement(33217..34593)
                     /locus_tag="PC621_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF4107223.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(34618..35169)
                     /locus_tag="PC621_000032"
     CDS             complement(34618..35169)
                     /locus_tag="PC621_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF4107224.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(35227..35442)
                     /locus_tag="PC621_000033"
     CDS             complement(35227..35442)
                     /locus_tag="PC621_000033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107225.1"
                     /translation="MQIHRELSKSENKYMCTKKEEQKCSKCEICGVLIDDTDKVYNNT
                     YNLCKKCKRNISTEKIVKYINIYDESK"
     gene            complement(35411..36244)
                     /gene="fdhD"
                     /locus_tag="PC621_000034"
     CDS             complement(35411..36244)
                     /gene="fdhD"
                     /locus_tag="PC621_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF4107226.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(36293..38437)
                     /gene="fdhF"
                     /locus_tag="PC621_000035"
     CDS             complement(36293..38437)
                     /gene="fdhF"
                     /locus_tag="PC621_000035"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(38021..38023),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDF4107227.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(38688..39746)
                     /locus_tag="PC621_000036"
     CDS             complement(38688..39746)
                     /locus_tag="PC621_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF4107228.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(39957..40616)
                     /gene="cprR"
                     /locus_tag="PC621_000037"
     CDS             complement(39957..40616)
                     /gene="cprR"
                     /locus_tag="PC621_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF4107229.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            40927..41556
                     /locus_tag="PC621_000038"
     CDS             40927..41556
                     /locus_tag="PC621_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF4107230.1"
                     /translation="MDENITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            41624..42457
                     /locus_tag="PC621_000039"
     CDS             41624..42457
                     /locus_tag="PC621_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF4107231.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(42553..43341)
                     /locus_tag="PC621_000040"
     CDS             complement(42553..43341)
                     /locus_tag="PC621_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107232.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(43342..44214)
                     /locus_tag="PC621_000041"
     CDS             complement(43342..44214)
                     /locus_tag="PC621_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4107233.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            complement(44229..46007)
                     /locus_tag="PC621_000042"
     CDS             complement(44229..46007)
                     /locus_tag="PC621_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107234.1"
                     /translation="MNLLNIVESIVSCIKGLARRGINMSSIKVISDFITKIIRKNILF
                     AITVIIINYHLIQRWTHISNRGISFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTV
                     KILCRNNFDYFLGISVAVTRNNFIFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILVFE
                     WFLPLTILSIVISFISVIINNLILKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKS
                     LNIFEDQSYAYYSDFVGKVNNSAYVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLI
                     VIVVYSILFIGLNYISIDMSTYRDEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNI
                     KDRIVLLNTSNEELTNINMILDEIFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPND
                     EVTLDIEYEGYINVLTSWNQNKYIATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSK
                     NNVYSNLPLLNKTSNFLKKDYIFEGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMD
                     IEEKIKFLIETRNEILGDSKASVDAKKYVDEVDNVMESIKRKKINMILVADLPSNEFL
                     YEDSKGEKISITIYHWLSGNILIIDK"
     gene            complement(46084..46665)
                     /locus_tag="PC621_000043"
     CDS             complement(46084..46665)
                     /locus_tag="PC621_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF4107235.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            complement(46724..46864)
                     /locus_tag="PC621_000044"
     CDS             complement(46724..46864)
                     /locus_tag="PC621_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF4107236.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            complement(46876..48192)
                     /locus_tag="PC621_000045"
     CDS             complement(46876..48192)
                     /locus_tag="PC621_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF4107237.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            complement(48186..48896)
                     /locus_tag="PC621_000046"
     CDS             complement(48186..48896)
                     /locus_tag="PC621_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4107238.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            complement(49334..49909)
                     /locus_tag="PC621_000047"
     CDS             complement(49334..49909)
                     /locus_tag="PC621_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF4107239.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(50202..50480)
                     /locus_tag="PC621_000048"
     CDS             complement(50202..50480)
                     /locus_tag="PC621_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107240.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            complement(50789..51550)
                     /locus_tag="PC621_000049"
     CDS             complement(50789..51550)
                     /locus_tag="PC621_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF4107241.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            complement(51719..53758)
                     /gene="bclA3"
                     /locus_tag="PC621_000050"
     CDS             complement(51719..53758)
                     /gene="bclA3"
                     /locus_tag="PC621_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF4107242.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            complement(53825..54901)
                     /locus_tag="PC621_000051"
     CDS             complement(53825..54901)
                     /locus_tag="PC621_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4107243.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            complement(55223..55807)
                     /gene="clpP"
                     /locus_tag="PC621_000052"
     CDS             complement(55223..55807)
                     /gene="clpP"
                     /locus_tag="PC621_000052"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF4107244.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            complement(55804..56262)
                     /locus_tag="PC621_000053"
     CDS             complement(55804..56262)
                     /locus_tag="PC621_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4107245.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            complement(56485..57900)
                     /locus_tag="PC621_000054"
     CDS             complement(56485..57900)
                     /locus_tag="PC621_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF4107246.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            58015..58683
                     /locus_tag="PC621_000055"
     CDS             58015..58683
                     /locus_tag="PC621_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107247.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            complement(58859..59377)
                     /locus_tag="PC621_000056"
     CDS             complement(58859..59377)
                     /locus_tag="PC621_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4107248.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            59529..59852
                     /locus_tag="PC621_000057"
     CDS             59529..59852
                     /locus_tag="PC621_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4107249.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            complement(59857..60441)
                     /locus_tag="PC621_000058"
     CDS             complement(59857..60441)
                     /locus_tag="PC621_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF4107250.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(60490..60945)
                     /locus_tag="PC621_000059"
     CDS             complement(60490..60945)
                     /locus_tag="PC621_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF4107251.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            61828..63366
                     /locus_tag="PC621_000060"
     CDS             61828..63366
                     /locus_tag="PC621_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF4107252.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            complement(63638..64351)
                     /locus_tag="PC621_000061"
     CDS             complement(63638..64351)
                     /locus_tag="PC621_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4107253.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(64806..66185)
                     /locus_tag="PC621_000062"
     CDS             complement(64806..66185)
                     /locus_tag="PC621_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4107254.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            complement(66470..67264)
                     /locus_tag="PC621_000063"
     CDS             complement(66470..67264)
                     /locus_tag="PC621_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF4107255.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            complement(67261..68124)
                     /locus_tag="PC621_000064"
     CDS             complement(67261..68124)
                     /locus_tag="PC621_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF4107256.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            complement(68102..69097)
                     /locus_tag="PC621_000065"
     CDS             complement(68102..69097)
                     /locus_tag="PC621_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4107257.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            69800..70756
                     /locus_tag="PC621_000066"
     CDS             69800..70756
                     /locus_tag="PC621_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF4107258.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            complement(71157..71705)
                     /locus_tag="PC621_000067"
     CDS             complement(71157..71705)
                     /locus_tag="PC621_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107259.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(71807..72505)
                     /locus_tag="PC621_000068"
     CDS             complement(71807..72505)
                     /locus_tag="PC621_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107260.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            complement(73041..74108)
                     /gene="rsgA"
                     /locus_tag="PC621_000069"
     CDS             complement(73041..74108)
                     /gene="rsgA"
                     /locus_tag="PC621_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF4107261.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            complement(74105..74506)
                     /locus_tag="PC621_000070"
     CDS             complement(74105..74506)
                     /locus_tag="PC621_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF4107262.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      75302..75398
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            75452..75628
                     /locus_tag="PC621_000071"
     CDS             75452..75628
                     /locus_tag="PC621_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107263.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            76038..76676
                     /locus_tag="PC621_000072"
     CDS             76038..76676
                     /locus_tag="PC621_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107264.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(77154..78830)
                     /locus_tag="PC621_000073"
     CDS             complement(77154..78830)
                     /locus_tag="PC621_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4107265.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(78931..79083)
                     /locus_tag="PC621_000074"
     CDS             complement(78931..79083)
                     /locus_tag="PC621_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107266.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(79572..79982)
                     /locus_tag="PC621_000075"
     CDS             complement(79572..79982)
                     /locus_tag="PC621_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4107267.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(80506..81825)
                     /locus_tag="PC621_000076"
     CDS             complement(80506..81825)
                     /locus_tag="PC621_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4107268.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKTRLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
     gene            complement(81822..82475)
                     /locus_tag="PC621_000077"
     CDS             complement(81822..82475)
                     /locus_tag="PC621_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4107269.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(82573..83949)
                     /locus_tag="PC621_000078"
     CDS             complement(82573..83949)
                     /locus_tag="PC621_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107270.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(83949..84605)
                     /locus_tag="PC621_000079"
     CDS             complement(83949..84605)
                     /locus_tag="PC621_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107271.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(84615..85892)
                     /locus_tag="PC621_000080"
     CDS             complement(84615..85892)
                     /locus_tag="PC621_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107272.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(85911..86801)
                     /locus_tag="PC621_000081"
     CDS             complement(85911..86801)
                     /locus_tag="PC621_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4107273.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(86794..86889)
                     /locus_tag="PC621_000082"
     CDS             complement(86794..86889)
                     /locus_tag="PC621_000082"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF4107274.1"
                     /translation="MAMIYYGHSNGETKVVLDKAIRICMECIGIG"
     gene            complement(86968..88125)
                     /locus_tag="PC621_000083"
     CDS             complement(86968..88125)
                     /locus_tag="PC621_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF4107275.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            88459..88809
                     /gene="mobC"
                     /locus_tag="PC621_000084"
     CDS             88459..88809
                     /gene="mobC"
                     /locus_tag="PC621_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDF4107276.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            88815..90146
                     /locus_tag="PC621_000085"
     CDS             88815..90146
                     /locus_tag="PC621_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDF4107277.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(90182..90988)
                     /locus_tag="PC621_000086"
     CDS             complement(90182..90988)
                     /locus_tag="PC621_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HDF4107278.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(90978..91439)
                     /locus_tag="PC621_000087"
     CDS             complement(90978..91439)
                     /locus_tag="PC621_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDF4107279.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(91467..92534)
                     /locus_tag="PC621_000088"
     CDS             complement(91467..92534)
                     /locus_tag="PC621_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF4107280.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(92531..94153)
                     /locus_tag="PC621_000089"
     CDS             complement(92531..94153)
                     /locus_tag="PC621_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4107281.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(94356..94586)
                     /locus_tag="PC621_000090"
     CDS             complement(94356..94586)
                     /locus_tag="PC621_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4107282.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(94726..95385)
                     /locus_tag="PC621_000091"
     CDS             complement(94726..95385)
                     /locus_tag="PC621_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF4107283.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(95428..104157)
                     /locus_tag="PC621_000092"
     CDS             complement(95428..104157)
                     /locus_tag="PC621_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF4107284.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(104240..105043)
                     /locus_tag="PC621_000093"
     CDS             complement(104240..105043)
                     /locus_tag="PC621_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDF4107285.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(105128..106834)
                     /locus_tag="PC621_000094"
     CDS             complement(105128..106834)
                     /locus_tag="PC621_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDF4107286.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(106929..108470)
                     /locus_tag="PC621_000095"
     CDS             complement(106929..108470)
                     /locus_tag="PC621_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDF4107287.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(108445..108684)
                     /locus_tag="PC621_000096"
     CDS             complement(108445..108684)
                     /locus_tag="PC621_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4107288.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(108696..110828)
                     /locus_tag="PC621_000097"
     CDS             complement(108696..110828)
                     /locus_tag="PC621_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDF4107289.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(110832..113261)
                     /locus_tag="PC621_000098"
     CDS             complement(110832..113261)
                     /locus_tag="PC621_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4107290.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(113173..113562)
                     /locus_tag="PC621_000099"
     CDS             complement(113173..113562)
                     /locus_tag="PC621_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDF4107291.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(113566..113826)
                     /locus_tag="PC621_000100"
     CDS             complement(113566..113826)
                     /locus_tag="PC621_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4107292.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(113844..114707)
                     /locus_tag="PC621_000101"
     CDS             complement(113844..114707)
                     /locus_tag="PC621_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107293.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(114727..114942)
                     /locus_tag="PC621_000102"
     CDS             complement(114727..114942)
                     /locus_tag="PC621_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDF4107294.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(114946..115257)
                     /locus_tag="PC621_000103"
     CDS             complement(114946..115257)
                     /locus_tag="PC621_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF4107295.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(115421..117205)
                     /locus_tag="PC621_000104"
     CDS             complement(115421..117205)
                     /locus_tag="PC621_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDF4107296.1"
                     /translation="MIDKILKDIKGLFKVQDKAKFLKQNIPYLAFFYVGNIFSHHVRA
                     YTGGDVIDKIFQGILELNTMSFLPSIHPTDILMGVVVAVLIKIIVYTKGKNAKKFRQG
                     KEYGSARWGTKKDIEPYMDEKFQNNILLTQTERLTMNGRPANPKYARNKNVLVIGGSG
                     SGKTRFYVKPNLMQMHSSYCVTDPKGTIVLECGKMLEDNGYEIKILNTINFKKSMKYN
                     PFAYLRSEKDILKLVQTIIANTKGEGEKSGEDFWVKAEKLYYTALIGYIFYEAPREEK
                     NFATLLDMIDASEVREDNETYMNPIDRLFEALEKKEPTHFAVKQYKKYKLAAGKTAKS
                     ILISCGARLAPFDIQELRDLMSDDELELDTLGDRKTALFVIISDTDDTFNFVVSIMYS
                     QLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIATIRSREISASIILQA
                     QSQLKAIYKDNADTIVGNCDSTLFLGGKEKTTLKELSETLGKETIDLYNTSETRSNQK
                     SFGLNYQKTGKELMSQDEITVMDGGKCIFQLRGVRPFLSDKYDITKHKNYKLLEDYDK
                     KNLFDIESYMKRKGKAKLNRETVITRMQ"
     gene            complement(117202..117687)
                     /locus_tag="PC621_000105"
     CDS             complement(117202..117687)
                     /locus_tag="PC621_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDF4107297.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(117689..118711)
                     /locus_tag="PC621_000106"
     CDS             complement(117689..118711)
                     /locus_tag="PC621_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4107298.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(118797..119078)
                     /locus_tag="PC621_000107"
     CDS             complement(118797..119078)
                     /locus_tag="PC621_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107299.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(119097..120731)
                     /gene="rlmD"
                     /locus_tag="PC621_000108"
     CDS             complement(119097..120731)
                     /gene="rlmD"
                     /locus_tag="PC621_000108"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF4107300.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(120906..122666)
                     /gene="pyk"
                     /locus_tag="PC621_000109"
     CDS             complement(120906..122666)
                     /gene="pyk"
                     /locus_tag="PC621_000109"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF4107301.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(122718..123677)
                     /gene="pfkA"
                     /locus_tag="PC621_000110"
     CDS             complement(122718..123677)
                     /gene="pfkA"
                     /locus_tag="PC621_000110"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF4107302.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(123886..127455)
                     /locus_tag="PC621_000111"
     CDS             complement(123886..127455)
                     /locus_tag="PC621_000111"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF4107303.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(127612..128559)
                     /gene="whiA"
                     /locus_tag="PC621_000112"
     CDS             complement(127612..128559)
                     /gene="whiA"
                     /locus_tag="PC621_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF4107304.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(128576..129001)
                     /locus_tag="PC621_000113"
     CDS             complement(128576..129001)
                     /locus_tag="PC621_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF4107305.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(129070..130176)
                     /locus_tag="PC621_000114"
     CDS             complement(129070..130176)
                     /locus_tag="PC621_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF4107306.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(130188..131045)
                     /gene="rapZ"
                     /locus_tag="PC621_000115"
     CDS             complement(130188..131045)
                     /gene="rapZ"
                     /locus_tag="PC621_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF4107307.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(131076..131789)
                     /locus_tag="PC621_000116"
     CDS             complement(131076..131789)
                     /locus_tag="PC621_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF4107308.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(131793..132707)
                     /gene="murB"
                     /locus_tag="PC621_000117"
     CDS             complement(131793..132707)
                     /gene="murB"
                     /locus_tag="PC621_000117"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF4107309.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(132980..133750)
                     /locus_tag="PC621_000118"
     CDS             complement(132980..133750)
                     /locus_tag="PC621_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF4107310.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            134068..135549
                     /gene="cls"
                     /locus_tag="PC621_000119"
     CDS             134068..135549
                     /gene="cls"
                     /locus_tag="PC621_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF4107311.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            135867..136364
                     /locus_tag="PC621_000120"
     CDS             135867..136364
                     /locus_tag="PC621_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF4107312.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            136381..138267
                     /locus_tag="PC621_000121"
     CDS             136381..138267
                     /locus_tag="PC621_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF4107313.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            138290..140071
                     /locus_tag="PC621_000122"
     CDS             138290..140071
                     /locus_tag="PC621_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF4107314.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(140211..142007)
                     /locus_tag="PC621_000123"
     CDS             complement(140211..142007)
                     /locus_tag="PC621_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF4107315.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(142221..143150)
                     /locus_tag="PC621_000124"
     CDS             complement(142221..143150)
                     /locus_tag="PC621_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF4107316.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(143150..144967)
                     /gene="uvrC"
                     /locus_tag="PC621_000125"
     CDS             complement(143150..144967)
                     /gene="uvrC"
                     /locus_tag="PC621_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF4107317.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(145226..148051)
                     /gene="uvrA"
                     /locus_tag="PC621_000126"
     CDS             complement(145226..148051)
                     /gene="uvrA"
                     /locus_tag="PC621_000126"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF4107318.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(148070..150040)
                     /gene="uvrB"
                     /locus_tag="PC621_000127"
     CDS             complement(148070..150040)
                     /gene="uvrB"
                     /locus_tag="PC621_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF4107319.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(150227..151489)
                     /locus_tag="PC621_000128"
     CDS             complement(150227..151489)
                     /locus_tag="PC621_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF4107320.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            complement(151552..152856)
                     /locus_tag="PC621_000129"
     CDS             complement(151552..152856)
                     /locus_tag="PC621_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4107321.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(152887..153747)
                     /locus_tag="PC621_000130"
     CDS             complement(152887..153747)
                     /locus_tag="PC621_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF4107322.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(153791..154660)
                     /locus_tag="PC621_000131"
     CDS             complement(153791..154660)
                     /locus_tag="PC621_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF4107323.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(154644..155714)
                     /locus_tag="PC621_000132"
     CDS             complement(154644..155714)
                     /locus_tag="PC621_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107324.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            complement(156169..156285)
                     /gene="rrf"
                     /locus_tag="PC621_000133"
     rRNA            complement(156169..156285)
                     /gene="rrf"
                     /locus_tag="PC621_000133"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        48443 a        28357 c        18521 g        61041 t
ORIGIN      
        1 atcattttaa ttaatttaga tgtatatact ataaaaatta aataaaaata gagactacac
       61 tttaccatgt agtctccttc tttctggtca tatttagaaa ttcacattta ttgttactgc
      121 ttttccgcct ctatttactg ttatttttgt actagcacct tttgagaaag tatacaactt
      181 tttatttaaa tcattcatac ctgttatatc tgtatctcct actttagtta taatatcccc
      241 tacctttact ccagcttttt ctgctgatga accagatatt acttctgcaa cataaacacc
      301 tttatcagta cttaattttg tacctgttgc tgcttcataa tttgatacat ctgtaccttt
      361 tatgcctaaa gttacttttt catattttcc atttttgatt acttcttcaa ctatactctt
      421 agctgtattg attggtattg caaatcctaa tccttctgct tgagaagctt ttgctgtgtt
      481 tataccaatt acttgacctt tttgatttaa caatggtcct ccactatttc ctgcatttat
      541 acttgcatca gtttgtaata aaccagtcat attagttttt tctgtttgta tagttctatc
      601 taatccactt attatccctt gagtaactgt tttttgaaaa tctaatccaa gaggattacc
      661 tattgctatt gatatatctc caacttttac tttgtcacta tcagcaaatt ctgctggtgt
      721 tagtccagtt ttatcaacct taactatagc taaatcaagt tgttggtcaa accatactac
      781 tttacctgaa gttgttgaac catcattgaa taatacattt acacttgtag cttgaccatc
      841 tgatataaca tgagaattag taagtatata tccatttgaa tcaactatga ttccagttcc
      901 tacaccttgt gtttcagttg gtattgcaaa catattacta gtatcaacac tagttgttgt
      961 aatacctaca acagatggcg tagctttttc tgcaactgct tgataaatat tttgactctt
     1021 accatcatca tttacaacta tcggcgtaga acttcctgta gatttgctta ctaaattgtc
     1081 cttaactaaa attattgtta gaaaactact aagtattgaa cttattatag caactaatat
     1141 tactaaactt atccctttct ttcttcttga catctcatac cactccctat cttaatataa
     1201 tataaattat caaaatatat ttttcccaat ttttcataaa aaaaatgaaa tcatctattt
     1261 tgagtaaact ccttttcgat gatttcatta tattatttta atcttaatta aaccttaact
     1321 atttaaaatt atttaagtct tttttctagc tcttcctttt ctttttcata acctggtttg
     1381 cctagtaaag caaacatatt tttcttgtaa gcttctactc ctggttggtc aaatggatta
     1441 actcccaata aatatccact tattccacaa gccttctcaa agaaataaac taaatatcca
     1501 aaattatact catctagttg aggtatattt attactaaat taggtacttt tccatctgta
     1561 tgagccaata gagttccttc aaaagcttta tgatttacaa aatctacagt cttacctgct
     1621 aaataattta aaccatctaa gtctacttcc tctgaactta tagttatatt tctcttacag
     1681 ttttcaacat taagtactgt ctcaaataaa agcctttttc catcttgtat gtattgaccc
     1741 atagaatgta aatctgttga gaaatctaca gaagcaggga ataaaccttt attttctttt
     1801 ccttcacttt ctccataaag ctgtttccac cactcactta cataatgtag ttgaggttca
     1861 tagttcacta ataactctac atcttttcct tttctatgta aaattgttct agcaacagca
     1921 tatctataac tatcattact ttttaagtca ggattttgaa atgcttctct agcatcattt
     1981 gcgcctttca tcatagcatc aatatcaagt ccagcagcag caataggaag taacccaact
     2041 gctgtcaata cagaaaaacg accacctaca tcatctggta ttacaaaagt ctcatatcct
     2101 tcttctgtag ctaactgtct aagtgcacct ttacttgcat ctgttgttgc ataaattctc
     2161 tttcttgctt cttccttacc atactttttc tctaagtaat ctttaaatat tctaaaagcc
     2221 agagctggtt ctgtagtagt tcctgattta gatattacat ttacagaaat atcctttcct
     2281 tcaattatat ctaataaatc cataatatat gttgagctta tattatgccc tacaaaatat
     2341 acttctggag cctttctttg ctctttttta agtccattcc taaaagaatg acttaccata
     2401 tctatagcag ctctcgaacc taaataagag cctcctatac ctattaccag aagaacatct
     2461 gagtctgatt ttatcttctc agcagatttt tttattctgt caaactcttc tttatcataa
     2521 tttttaggaa ggtctatcca tcctaaaaaa gtatttccta aaccagtttt attatgtaac
     2581 atgtcgtgcg caacatctac atatggttgc atgacatcta tttcgttttg acaaaaaaac
     2641 tctgtagctt tactataatc aaaatttatt tttcccattt aaatccctct tttcagcata
     2701 ttcgttttaa agagattata acacaaaata taggtgtttt ttttaaaagt ttgccacaaa
     2761 atcatacata tctcatcatt tattatcagg aaatcgattc ttctaaatac tataactatg
     2821 tataaattta aaaattttag tcaaataaat ttacaaaaaa agaaactcta aacgtatcat
     2881 ttaaagtttc tcttttattt atcctctatt ttaaatttta tattatttta aattaattat
     2941 ttttaatcat ttcctaatct cttcgctata acttgttgtg ctatattgac agcatggtca
     3001 gatattctct ctaaattact tattacgtct aaataaataa ctccattttc tatactacat
     3061 agattattgt tcaatctata catgtgatta gctctacaag atttctccat tatatcaact
     3121 tgttcttcaa tttttataac cttacaagct aaatcaacat tatcagttct cattgattct
     3181 aatgcataag tatatgctga aacaactcta ttatacataa catcaagttc agattgtcct
     3241 tcttctgaga aagaaatatt tgaatctatt gcaacttgcg ataattcagc tatattttta
     3301 gcgtggtcac caattctttc tatatcattg actgtattaa acaaagtatc cacaacttct
     3361 cttgaatcat catctaaagg tgcttttgaa agtttaagta agtaatttaa aatagctttc
     3421 tgtaaatcat tgattaatct ctctcgtcta tatgtcttag ctattttttc agttgaatgt
     3481 tctactaagg cttccataga tgcatttagg ctttccttcg ccttctcacc catacgaagt
     3541 gcttctttta tcgtatttgc aagagctata gaaggagttt ctatcattct atcatctata
     3601 tatttaatag ttttagtttc atcttcatct atatcacctg ctctatcagg aacaagcttt
     3661 atagctagtt ttataataag atttgtaaat ggtaaaagta tcaatacaga tattatatta
     3721 aacaatgtat gtgtattggc tatttgtctt gcaacattgc ttggatccca acttgtaacc
     3781 atgcttacta ctggtttatt caagaatatt aagaatatca ccgttcctaa aacattgaag
     3841 attagatgca tcatagctgc tcttcgtgca tttttgttag caccaatact tgataacagt
     3901 gaagttacac atgtaccaat attttggcca taaagaattg gcagtgctgc tgataatggc
     3961 actagacctt ctgctgccag tgcaaccaac atacccatag atgcacttga actttgtatt
     4021 attgctgtaa tcccaaatcc caataataat ccaagtagtg gatatctacc aaatgtaaca
     4081 agtaggtcag taaatccttg atagtctctt agtggctcca ctgccatctt cataaaatcc
     4141 atacctgtaa ataatatacc aaaacctata agtatttcgg ctatattttt tattctaggc
     4201 ttatttccaa ataaataaag tataatacct acacctaatg ctactggggc cataccagtt
     4261 aagtcgaaag ataccaattg ggctgtaaca gtagtcccta tattagcacc cattataact
     4321 cctattgcct gttttaaagt cattattcct gcatttacga aaccaacaac cattacagta
     4381 gtagcacttg aactttgtat tactgctgta actcctgtac ctaccaaaac tcccataaat
     4441 aggttactag taagtagttt tattatcttt tttagctttg tcccagcaga tttttgaaga
     4501 ccttctccca taaggttcat accatataag aacagaccta atccacccat tagacttatt
     4561 ataatgttca aattatttcc tccttgtaaa gagattactg tatctaaaaa ttttataacc
     4621 tattgtattt tatcaaatta taggagtgat aatgttaaat tcatgttaaa tattaaaaaa
     4681 tcattaagtt tatgttaaat ttttgtatta ttggtgtatt tttgtattat tttttctgca
     4741 cattttatac catcaactgc tgctgtaact ataccacctg catatccagc gccttcacca
     4801 catggatata gattcttaat actcactgat tctaaagtat tttcatctct tactattctt
     4861 attggtgatg atgacctagt ttctactccc gtcaaaacag catcatacat tgcaaaccca
     4921 ttcaattttt tatcaagaga tataaacgct tctctcatag tctctgttac gaagctaggc
     4981 aaacattcac ttaaatttgt aagtgtatat cctggtctat atgatggctc aacacttcct
     5041 aaattcaaag aagtcttatt gtttataaaa tctccaacaa gctgaactgg tgcattgtaa
     5101 ttttttccac ctaattcaaa agctaatttt tcatacttct gctggaaatg aacacctgct
     5161 agtggattat cactcttaaa atcttctggc gttacattta ctaaaaatgc actatttgca
     5221 tttattttat ctcttgcatg ttcactcata ccatttgtaa ctatttcaaa atcatttgat
     5281 gcagatgcta tgacactacc tcctggacac atacaaaaag tatatgctgt tcttccattt
     5341 gaaacatgct ctataagtct ataatctgca gctcctaacc taggatgatt ataaaaatcc
     5401 ttgtactgtg ctttatttat taaatcttgt ggatgttcaa ctctagctcc tattgcaaaa
     5461 ggtttttgaa ctatttttat acctcttgaa tacaacattt cataagtatc tcttgcacta
     5521 tgtccaacag ctagtataat tgtatctgta tctatttttt cagtatcatt tattattaca
     5581 gattgtatag aatcatttga tacaactata tctgttactt ttgaattaaa tctaacttga
     5641 ccacctaaac ttattatttc atttcttata tttttaacta cattttttaa tatatctgtt
     5701 ccaacatgtg gcttatgtga gtataatatt tcatctggtg ctccaaactg aactaactct
     5761 tctaatactt ttctacatcg tatatctttt attctagttg taagtttacc atccgaaaaa
     5821 gttcctgctc caccttctcc aaattgaacg tttgaattat tttttaattt tctagttgtc
     5881 caaaaatcac ttatatcttt ggttctactg tctacatcaa gacctctttc aagtatgatt
     5941 ggttcataac ccatttgagc taaaagcaga cctgcaaata aaccagctgg tccactacca
     6001 attaccaaag gattattctt taatttttta tcacccacag gtacatttat gtatctgatt
     6061 tcctttattt ttgttacatc ttttattttt gagttaagta ttttattttc atctttgact
     6121 tctacatcta cagaataaac aaattccatt tttccttttt ttctagcatc tatagattct
     6181 ttatatatac tatatttgac taagtttttt tcttttattt ttagcttctt aagtacaaca
     6241 tcttttaata aagatatatc tttatcaatt ccaagtttta cattagatac tcttaacata
     6301 ttgttttcct ctcttagatt gtaatataaa aaagctaggg ttattcctag ctttctacaa
     6361 atataacttc tatattctct tttactagat tttctaccca aatctcatct agcacaaaat
     6421 tctttatttt gtttacaaca agtgttttat ctaaatcctt tttcaagttt tttactaaga
     6481 ccattgcttc aactgattta tgcaatcttt caacatacag agaattaact ttaatcaata
     6541 caacccttaa ttcacacaca tataactttt cactacctaa ggtgatttca tcatcatatt
     6601 catatacact aaattcatat gtattttcat aaggtgttat attttctatt cctatatagt
     6661 ccatttattt atcctttttt agttataaga cctcttataa ccttcatgtt attaaacacc
     6721 ttttctaaga agttctctgc ttcttcattg tctaaaaggt ttatttcaag gttttcgttt
     6781 tctacaatac ctttagattt acagtactct ttagcaacat ttattagttc atttttttta
     6841 tccatagata aatcaaactc tttatagtct attattatat attgtttggt atcaggtgat
     6901 acaccattat tatatcctat ttctatatga taaataactg catatatttc ttctccgttg
     6961 tacacaggta cagctgggtt agattttata aagttcgttg atacagcaat tgcatttcct
     7021 aaactaagac tcattattaa tacctctttc taaacattat acatatatta tactatagat
     7081 tctttatcta tgacttaaag ctttttataa ttaattaaga tatttttaaa tctttgtatc
     7141 tctttcctag tgtactagag cttatatcgt atttctttga tatttgaact tgagttatag
     7201 gttgtcctgc ttctttttta acatagtatt caacagcagc tgcccaacca ttttcagctc
     7261 cttttatatt ttcatgattt ttcttatatt cattccaaaa ctctatacat gcatctatat
     7321 tttctttttc tacattttct ttaagagtat ctaaaacttt acattcttct tcagctaatt
     7381 cattttctga attaactttt gtatcgtttt tatctctttc tctctttaaa tattcagcta
     7441 tacttgggtc taaaagctgt tgtatatact tgtatagtat atgtgtatga taaattaaga
     7501 acgacttcat atctttgtat acatcttgat attgattgaa tagattcata atatccttta
     7561 aaattatatc tttaacctca tttgatatac ttatagttat atctatcaat atgttagttc
     7621 cttcaatatc aactattcta gctatcatac aacttccgat tttaaaactt ctcagaagtt
     7681 ttacatcttc tgtacaaagt tcatttccac ttaaacagtc ctttaatact atttttccag
     7741 cagaaacttc ttttacctca taaatactta cgtatgattc aaacatactt tttaatattg
     7801 atacttctga ttgacttagg tttgcaccat tttcttcaag aaaaccaact gtcataacct
     7861 ttttattttc cattatgtgg tcttgtatta aataagtatt aaaaaatctt tcaaattttt
     7921 catttacact atcattttgc attatataaa acatttcttt agctttttca cattcttctt
     7981 taaacttatc ctgtcttgaa taatcatata actttgaata taaatctgta tattgttttt
     8041 gtgaaagttc agtttttctg catgctcttt ctgaaacaat atctttattt aaacaacatt
     8101 tcttgtattt tttgccactt ccacaagggc atagctcatt tctccctaac aatatgactt
     8161 ccactccctt attattaaat taaagtacaa tttgttatta taccattttt ttattaatca
     8221 atctatagta taagtaaata ttctattaaa ttcacttata ttttggtaca atatattaac
     8281 tttaattagg atttcgataa aatcatcaac aatccctctt gtagaaatga attatcgtaa
     8341 aactaattcc tctataaaat tttattaatt gataatagtt ttttacttta tctattcttt
     8401 tttgttatat gactctaact gtcattttac attcaacaat attgtctttt tcttgtttat
     8461 attcatttgg aatattttga acatcaaaat tattgacttt taatttgtta atttctatct
     8521 tttcaagttt attatttagt cttaacttct tcattacacc ttcaagtttc aacacatttg
     8581 caagttctcc attaactttt atttctaaat taacttgttt tttattgtga ttatctaaac
     8641 tactttcttg attaatatat tctacattca caatatctct cagttcattt tctactaata
     8701 acactatatc tttatttgtt tttttatcta aaaatgagtt attaaaatta tttttcaatt
     8761 cttcattttt atttaattca acactttcta tatatttatc atctatatgc ttattatcct
     8821 ttatttgata attatcttga ttatttattt tatttattat attttgcttt tcattcttca
     8881 aactagttag gttttcttcc aaataactta ctgctatatc acttgccaat ataataacta
     8941 atattaacaa tattttaagt agattttttt ctctattatt taatctcaaa aataatcacc
     9001 tatctaactt tttattctaa atctataata ttctccatct cttacaatac tgtctatgta
     9061 ataatatttt atgtacttta ttttccttag gttgtttaaa acatctaaat cactacacac
     9121 accttccatc tctagcttat ttttatctat cgatatttta gtaatactat ttattccaat
     9181 tgtttcatat atttttttta tttcattaag ttttatttta gaaaatttct ctctagtttt
     9241 atctgttgtt ggtaaagtgt gctttgattt atccactagc ttattttgat ttatataatc
     9301 ttcaagttca tttatagatt tcacatacat aaatgattta aataaaatac acgttaaaat
     9361 taagactatt attattttat agattttatt acatggttta gtttttttaa atccattaag
     9421 aaaatttatt gttttatcca ctttcactta aataatcatt cctatactat ttatataatt
     9481 tatcctattg tcttctaaat ctgcatcaaa attattttct acttttactt tatcttttaa
     9541 tcttaatgga tttacttcaa tagatttatc tgtatattta aagtattctt caataaaaca
     9601 attttctata ccatagtaat aaacatactt aaaatccctt aataatcttt ttatgaactc
     9661 atatgatttt ccctcttttg atagcaagta tgtttctctt atttttttat cttttgttat
     9721 atttataatt aactgacttt ttttacattc tataaatacc ccttcttctg aaaaattctc
     9781 tattaaattt aagtttataa gcttttgtaa tatatcaaaa tttatattta tagtctttgg
     9841 attcatattt agattttctg aaatgttctt aattaattga ataatactct ttgggaaaag
     9901 aattacttgt atattcaatt tatcattttt agaatttata actttatatt ttattgaata
     9961 gttatttatg tttattggaa tgtattgtgt tatttcaaat tcaatcatgc ttaatatgtc
    10021 tctttttttc ttagtattta gaacttcaac atttctaatg actatatctt gaatttgaat
    10081 gttaaagtat aaatttttat ataatatttt tgatttacca atctcctctt ttacaaatct
    10141 aactacatct tctatatatt taatcttctc aatcgaatta ctatcatttt ttatatcaaa
    10201 gttaatctct tttataattg atttatctat aaaaactttt ttatgacttt ttcttaaaaa
    10261 tacaagcctt atataatcat catttatttc taagtttaga atttttttaa ttatagccat
    10321 ctccttctta aataaaaata attttaaaac ttacagcaat caatataaaa tattattttg
    10381 gataaaattg tttagaattt atatttacgc ttatatctgt tccgctatca tttatgacaa
    10441 gttcgaattt acctgatgta ctctgaggaa ctggaacagt atttaggtat cccttttcct
    10501 tcaatgtttc tacacttaga gagtctattg tcttttcatc atcattaatt gccatgctag
    10561 ctgcagttac tatattagca gcattcgtat agtctgcatt taatttggca ctttcttgaa
    10621 ccttactata cttaactaat gctatactag atataacgcc taaaatagtt actactacaa
    10681 tcatttcaac aagtgtaaat ccttttcgtt ttttatttat caaatttatc aattaaatat
    10741 tctctccttt tcaaaagttt catctaataa aatttatttt tctctctaaa aattagtaat
    10801 tgcatcaaac ataggaacaa ccattgctaa aataaaagtg ccaataatta atcccataaa
    10861 tacaattagc ataggttcta taaatttcat tatttgctct attttgttat ttaattcatt
    10921 ttcataaaat ttatttatta catttagact tttatctagt ttcccacttt cttctcctgt
    10981 atttgccatt gaaataaata attttggaaa aacatttgct aaattcaaag atttacctat
    11041 gctatttcct ttttttatat aattattaga aatctttaac ctttcataaa taaattcgtt
    11101 gtctataaca ctagctgaca tgtctatagc ttccacaatt tgtattccac tgctaattaa
    11161 aacggacaat gcccttgaaa atcttgttgt agctactaac tgattaattt tttttataaa
    11221 aggtattttg aattttaacg aattaatcaa tttttttata ttatcatttg ttataaagaa
    11281 ataaatgctt cctattgaaa acaatataaa actaaatatt atataagcta agttatttgt
    11341 aaccactgta gatattccca ttaaaactct agttattaaa ggtggttcaa ttccattgtt
    11401 ggtaaaaacc acctgaaaat taggtattac aaatacgaat ataaacaaca ttgataagat
    11461 agataatatt ataagtatta ttggatatat tgaaatagtt attattttca tttttaattt
    11521 gtattcttta tcataataat tagataaatc actcattatt atatctaagt ttccactagt
    11581 ttctcctgct tttatcatac ttataaagaa tttagaaaat atacctgtta tttgaaatga
    11641 ttctgctatt gagttacctt tttgaatatg atttgatact atactaagca aattctttac
    11701 ttttttactt gactgactct caatgatttc aaatattttt gtaatttcac atccagattc
    11761 cagcaatatg cctatctctt tgcaaattac tctaatttct ttatcactga ttctatttaa
    11821 tactaaataa aacttttcta aattataaaa tatattttta gataatctca tgtactataa
    11881 taccttccaa attatattaa ttacaattaa ataaacttaa tattttctaa tttaatgcat
    11941 ttaattttat ttcacttaaa tataatttat actcaaacat cttaattaaa atagattaaa
    12001 taaagacaac tgaccaaacg aagtttaact atacaattca gttaaatcat ttcatttact
    12061 actaaacact cttctaatgt cgttattttt tcatttatta gtcttttaca gctatcttca
    12121 aatgttatca ttccattatt ttgtgccaca tctttaatag tactagaatc ctccatattt
    12181 cttatacatg tctttatatc atcattaatt tctaagattt cataaattgc tgttcttcca
    12241 aagtatcctt tatcattaca tttttcacac cctacagcta ccttagtatt tacatctcca
    12301 ctaaaattat cttttatcat aatctcatgg ctacaatgat tacaaacttt tctaaccaac
    12361 ttttgagata taacacctat aagagaggca tttaaaagat atggtggtat ttgcatttct
    12421 agtaatctag ctatggaaga tactgcatca ttagtatgta aagtacttat aactaaatga
    12481 cctgttatag ctgctcttac tgctatctta gctgtttctg catctcttat ttctccgacc
    12541 attattatat ctgggtcttg tcttaatatc gctctaagac ctacatcaaa gctcaaccct
    12601 acttttgaat ttacttgaat ctgacttatg ccatccatct tatactctac aggattttct
    12661 atagtcataa tatttttatt tattcctttt aaatcattta ataaggaata tactgttgta
    12721 gtctttccac tacctgtagg acctgtcact aataatactc ctgctctttt atttattata
    12781 cgatttatag attttatagc ttcctctgaa aaaccaagtt ctaatttgtc ttttaaaaaa
    12841 gtatctctat taagtatcct caaaactatt ttttcaccat agacagttgg tattgatgaa
    12901 actctaatgt caattaaatt tccacctaac tttatatcca ccctaccatc ctgaggaagc
    12961 cttttttctg ttatatccat tgatgccttt aattttataa ccgtagacaa tgatggatag
    13021 acatcaacta aaaactttga tacttccttt agttctccat ctaccctcat tctcactatt
    13081 aaatgttctt taaatggttc tatatgtata tcacttgcat tttcttttat agctttttct
    13141 aaaagatttt caaataattt ttttgcataa tcctcatcaa gttcaaagct attataatat
    13201 ttttcaatct tttcacttat aagttctttt ttctccttct tcaatataat ttctttacct
    13261 gtagctaatc tcaagtcctg tagagcataa atatcctcat cttctatttc tacaactaaa
    13321 ttatcatctt gaaactcaac tggaaataca ttatatttct ttgcaagttt ttctggtatt
    13381 aacattggca aactgctttc ctctaatata atattaatca ccctttatat actttaattt
    13441 attaggtata ttcatttttt tcatagagat attttcttca gtttttatgc ttattttcat
    13501 tggataattg gtttgtatgc tatctaaata tttactacct ttatctacat aattagataa
    13561 tttctcctta tctccaatgc actttatttc atatggttgt gctacaggaa ctccatttat
    13621 atttatatgg cttcctgcaa gtacaacttc tgaatattga ttaattcttt gaccatttat
    13681 ttctatgtat tttcctccgt ttacttttaa ttcatttata acttttacaa gaatcttatt
    13741 atagtctata ctattagcta tattccctgt tttttcatct atagcatcta tacttaatat
    13801 tatcccacta ccctttacat ctgaatatga tagactatct ctaagtgttt ctatttctga
    13861 tatttcttca gaattttttt gattttgttt cattgaatta atttctttat ctaacttgtc
    13921 tttctctttg gttaattctt ttatagattt tcttgtcatt tttatttctt ttcttattga
    13981 cttgtctttg cttgtataaa ctgtttcttc attgctataa tctctaaata tggttccaac
    14041 aagtatacct aaaatagtac ttgctagtat tataaacaca tattgttttt tcatactgta
    14101 ttgctcctta caaatttatt tttatatgat aattttcttt aattaaatat taaacattta
    14161 catatgctta ttatatcaaa gttatatatt aaaattcatc tttagtatat aatatcatac
    14221 attgaactcc tatcactaat ttaccaatat caagtatgca tttttagatg ttcttaaaat
    14281 tatataaact ttcaataaac attgttgtga tattataaaa aaacataaat taggaaagga
    14341 aataataatt atgaaaaaca aataatggct ttggagatta ttaatatcat tttttagcaa
    14401 aaaaaagtaa aatggcatag cctcaaagct acaccaattt actaaaaatt atattattat
    14461 taacattaat tttaaacata tacgtttata agttttcctc atattttaaa attcttttaa
    14521 ttaacttatc tcttaatttc cctacttcat caaatctcat attaggacta tagtaacttt
    14581 ctataatatc atgtttagac ttggctctct taagatttag tactgcccaa caaattaaat
    14641 catctaaaac tttttcatca aatttaatat cttcttcata tttaagtagt ttttctctat
    14701 ttaaaaactg atttaaatca attgtatctt cttctttaaa agtcgtactt atagttatgc
    14761 ctatatctaa atcagttaat agtacagttt caattttctc tggaataagt ggataatgat
    14821 aaacttctac atttaaacct ttttcagtag ctttcttata tactttgttt aataatgtag
    14881 ttttcccagt tcctatttct ccatctaaat aatagacttt ctttacatct tttaaaatgt
    14941 tttctgtata atctaaacac cctacaggag ttatagctga accaaataaa tgcttttctc
    15001 tttttagatt tcctgtgctt ttaactccat caaataacct ttttataaat tcttctgtga
    15061 ccaaatttac tttgccataa tccatacaat cagaatattt ttcttctatg tctctaaaaa
    15121 ttggttctgc tgactttaaa aacttaaatg ctcttttaaa tgaattacta atatctttat
    15181 cataaactga tatttcttgt ttattctttt ctaattcttc tgtattccag taatcaccta
    15241 aattaattat ctcatccaaa gctccaggta gctttgggtc catagtatgt ggggcagttg
    15301 catccataag taccacgcct aatttcttta taactaaacc atccaacgaa cttgggtcag
    15361 aaggacaatg atataaatca atatcatatc ctctatgcaa aaactcttta taaatttttt
    15421 tcataagtga ggattttcct actccaggtc caccctttaa acaaaaaact ctttttacat
    15481 tttcaggaat tatatagtca aaaaaagaat atgagccttg tgatgtattt ccacctggga
    15541 atgccttctt tacctttcct tccatattag cgcctcctca taatttacaa tatctcttaa
    15601 ttaagatatt catattataa gaattttatt acagctttct gattttgaca aatttaaaac
    15661 ttaccatcta atactaataa catattaatt ttatctaatt attatagatt ctcatactgt
    15721 accttattct atctataaat acaatttaag tgtccatatt gaaatatttg tattgtaata
    15781 cagctggata ttacttaaat ccaattgttt ccattataat tttatgttaa aataatatta
    15841 caaaatacat ctgtttttct tcataaacgg gtgaaattcc ctatcggcgg taaaagcccg
    15901 cgagccttat ggcataattt ggtcatattc caaagccgac agtaaaatct ggatggtaga
    15961 agaaaatagt atatgagtac ctttatgtaa ttttacatga gtaatctata caaatccttc
    16021 aactaccgta tttattcatg aaattagaca cattcaaggt acctaatata caggtgcttt
    16081 ttttgttgtt tattttacaa ttatatcgta cttataaaat ctattaagat tggagtgtta
    16141 tcatgaaaca aaaatggata gtattgatta tcatctgtat tggtgtattt atgtctactc
    16201 ttgatggaag tatactaaat atcgcaaatc ctacaattgc tgctgacttt aaaattaaca
    16261 tgtcacaaat tcagtgggtt gtaacagctt atatgcttgt tgtaacagct actatgttat
    16321 ttttcggaaa acttggtgat aaagtaggtt caaatcgtct atacgcatta ggatttttta
    16381 tatttacaat aggttctttt ctgtgtagta tgtcaaacaa tttatcaaca ttaatttctt
    16441 caagaatctt tcaggcagtt ggagcaagta ttttaatggc tactgggctt ggtatagttt
    16501 ccaatgcatt tccagcaaat gaaaaaggaa aagctatagg aatcactgga gctgtagttg
    16561 gtataggaaa tatgagtggt cctgtaattg gaggtataat tttagaacat tttggatggc
    16621 cttctatttt tattatcaat atacctattg gtattatagc agtctttctt ggcattaaat
    16681 ttctgcctaa acctgttttg gatgaacaaa acaagagctt tgatatttca ggtcttctac
    16741 ttttcgcatc ttgcactacg cttatattac ttgcaatgaa tgagaagggt aatacaagat
    16801 tgtatttagg tataacagca cttataatct tcttattgtt ggcattaaga gaagtaaaat
    16861 ttgaacaaag ctttattgac ctacctctat ttaaaaatag aaactttact gtaggcaata
    16921 tcattggagt agcttgttat tttcctcaaa tggcagtatc ttttctactt ccattttatt
    16981 tagaacaatt aaaaaattta tctcctatga tggctggtta tgtaatgact gtacatccac
    17041 ttattatggt acttatagca cctattgcag gttcactttc tgataaacac ggtgcaaaaa
    17101 acattctaac agcatctttt tcatttatga ccatctcact agttggaatg gcacttttaa
    17161 aagcagattc tccattgtat cttttaattg tatgtttagt tatttttgga cttgggctag
    17221 gagcatttag ttcaccaaac aatagtagta ttttagcaga tgttcctcct caaaaacagg
    17281 ggtacggagg cagttttctt gcaactattc gtaatttatc atttgctctt ggaacagcat
    17341 tttttagtag tttttttgca caaagtctta catacaatca aaagtttaaa agtcacacat
    17401 cagcctatgt tatagctagc aatcagtctt attggattgc agcatcagta tgttttatag
    17461 ggcttatact aactgttttc tttatgagaa aaacagataa atctatttct taaatgctat
    17521 ttattgctga ctacataagt caataatttt ttagttgaac taatttatac taattttccc
    17581 ataccttact aaaatttatt agtatttagc aaaattactg atatttattg tttataaaca
    17641 ataaggatgc caaaaaaagt tcattaaaac tattttaagg catcctatat tttgctttaa
    17701 tattaatata attaaaacta actattatta catttattat tcaaataaat cttatgtcaa
    17761 tatttaacat tttaaattta atctactgtt atttcatatc catgttgatt gtattgattt
    17821 acaatctctt cccataattc ttcaacacca gtctttttta gtgaagaaac aggaattact
    17881 ttttcactac tttccatttg caattttttt cttattatac ttatgtgttt ttgatattgt
    17941 cctctagata ttttatctgc cttagttgca ataactacac agttatatcc aaagtgcttt
    18001 atccattcat acataagctt atcatctgct gtaggctcat gtctaatatc tacaagtagg
    18061 aatatagaac ataactcttg tctactttct agatatcttt ccattatacc tccccacttt
    18121 tctttttcag acttagctat ctttgcatat ccatagcctg gaaggtctac aaaataaaat
    18181 tcattattta ttagataaaa atttatagtt ctagtttttc ctggtgtttg actggttcta
    18241 gcgaagtttc ttctgttaag caatgtattt attattgatg attttccaac atttgaccta
    18301 ccagccaatg ctatctctgg gattccttct gctggatact gtgatttatt cacagcactc
    18361 atagttatct ctgaacttct aattttcatt attttcacca ctctttaata aagcttgttc
    18421 taatacttca tccatatgtt ctacaagcac aaactccatt ttttctttta cattttctgg
    18481 tatttcatct aaatcagctt cacattcttt aggaattaat acttttgtta taccagctct
    18541 atgagctgct aataactttt ctttaacccc tcctactgct aagactctac ctcttaaagt
    18601 tatctctcct gtcattgcta tatttccagg aacaggtctt ttagtaagtg ctgagattac
    18661 tgcaagagcc atagttatac cagctgatgg tccatctttt ggaacagcgc cttctggaat
    18721 gtgtatatga atatcattcg tcttataaaa ttcagggtct atatcaaact tgtccactat
    18781 tgaccttata taagagatac ctgtcttcgc agattccttc ataacatctc ctaattttcc
    18841 agttaataca atttctcctt tacctttaag tacgtttact tctacttcaa gtgtaactcc
    18901 accaactgct gtccatgcaa gaccatttac taatcctatt tgaggattaa cttctgcaag
    18961 ttgatactta aacatgtctt ttccaagata tgtctctagg tcagacttat ttataacaac
    19021 ctcttcaaga gttgggtctt caacgtattt tttggcaacc tttctacaaa tcttaccaat
    19081 agttctttct agtgttctta caccagcttc tcttgtataa tggttaatta tacttcttaa
    19141 agtttcatca tctattttta taaagttttc ttttaaagca tgttctttaa tctgttttgg
    19201 aagtaggtat tttcttgcta tatttaattt ttcttcttct atatacccag atacttctat
    19261 tacttccatt ctatctaata atggtcttgg tatattacct agactatttg ctgttgtaac
    19321 aaatagtatt tttgataaat caaaaggtat ttctaagtaa tggtccacaa agtccttatt
    19381 ttgctctggg tctaatactt ctaacattgc tgaagctggg tcacctttat aatctgctgc
    19441 catcttatct atctcatcaa ataagaatac aggatttttt gtttgggctt cttttacccc
    19501 attaattatt cttcctggaa ttgaacctac ataagttctt ctatgacctc ttatttctgc
    19561 ttcatctcta actccaccca aagatattct tacaaatttt ctatttaatg ctctggcgat
    19621 tgattttact atagatgtct ttcctgttcc tggaggacca actagacata ttataggccc
    19681 tttaagtgac ttagcaagag ttcttatagc tagatactct agtattctct ccttaacttt
    19741 ttctaatcca taatggtctt catctaatat atcttttgcc ttagttatat ctaatttatc
    19801 ttttgtctct ttattccatg gcaatgagaa tatagtatct aaatagtttc tacttacaga
    19861 tacatctgga gacatagaag atatttttga aaatttatct atctcttttt ctattttttc
    19921 tttggttgtc ttaggtgctt ttattttttt aagcttttct ctatactcat ctgcttcaga
    19981 atttatatct tcttcttcac ctaattcttt ctgtatagct tttaattgtt ctctaagata
    20041 atattctttt tgaaccttat tcatctgttt ttttactctt aaagttattt tcttttctat
    20101 ttttaatata tctatttcct ctaataatat agaataaata agttcaagtc tttttctaat
    20161 atcaaattct tctaatattt cttgtttttg gcttgatttt agatatatgt ttgcagctat
    20221 cgtatctata aatctatcta catcttctat atcagctaga gatattaata tttctggtga
    20281 taccctattt ccaatattta tatactcttc aaaggcatca aatacatttc ttacaaatgc
    20341 ttcaatttca acttctgttt cactatccaa attatcacta ttaaatacta tttcttctat
    20401 tactgctctg aaatacccat cttcttgttc tattttcttt actctacccc ttgatacacc
    20461 ttcaaccaaa acacgaactg tatctcctgg taattttatc atttgcttaa ctttacagat
    20521 tgttcccaca tgataaaaat cctcttctcc tggctcatct acctctgctt ctttctgaga
    20581 agttaaaaat ataagttctt catccatcat agcttggtct aaagctttaa gagatatctc
    20641 tctacctata tcaaaattta atatcatata tggaaatata gctaatcctc ttagaggaat
    20701 taatggtaat tcatgatcta ttttagtata attttgttcc atcaacatta ctccttttct
    20761 agtctaattg ctttgctttt aatattatat atttgaacaa gttaattttc aacattaatg
    20821 ataaattctt agaatactat catactatat tatcttttta ttatgatata atattaatat
    20881 tagtatactt agatgttatt tatcttttgg aggtttctat tatgttgaaa ttgtttttaa
    20941 cctttttaaa aattggtgcg tttacatttg gtggtggata cgctatggtt cctcttatac
    21001 aagatgaact tgtaaacaaa caaaaacttt tagagcagga agaatttatt gattatttat
    21061 ccatagctca aagttatcct ggagtactgg cagttaatat atctgtatta ttaggatata
    21121 aactttatgg tataccagga gttcttatat gtaccttagg ctctgcttta ccatcatttt
    21181 gtatagtctt agtcttatcg tatctatact ttaaaaattc atcatctaaa attttagatg
    21241 gattttttaa aggagttgcg cctgttgtaa tagctcttat attgtattct tttacgagca
    21301 tgttcaagaa actaccaaag tctaaaacta acatactact attaattata gcagttttat
    21361 ctgttggtgt attcaatata agccctattt atttaataat tggaggaggt gtatatagtc
    21421 tatgtcagaa ataatactac tcttcttaat attccttaaa ataggggcat ttagttttgg
    21481 tggcggttat gccatgctac catttatcca gcaagaattt atatctaaat atcactttat
    21541 aactaatcaa gagtttttgg atttacttgc tatatcacaa tctacaccag gtcctgtagc
    21601 aataaatagt gctacatttg taggatttaa gactgctggc ttctttggtt ctctagctgc
    21661 aactattggt gttacaattt ttgcattaat aggtctaact attatatcaa agttattttc
    21721 taaatttaaa aataataaaa aggtagaaaa cttattgaca gttcttagac caattactct
    21781 aggattcata ttatcagcag ctttttcatc tgccaaagaa gttaattggg atttatatgg
    21841 aataattgca tttatagcat cattttatct tttatataaa ggaaaaattg gttccattgc
    21901 aattgtattc gtctttggta ttctaggtat attactcaca tttttatgat aatgatacct
    21961 attttatatt tagcatatta acataaaaaa aggagctttt aaagctcctt ttcatcatct
    22021 tatgcacttt cctcttgatc cttaagtact atgataggat ttactgagtc ttcgttcaca
    22081 gatttctctg ttactataac cttctttata ttatctctag atggtacttc aaacatagtt
    22141 tccatcatta cgctttcaac tatacttcta agtcctctag ctcctgtatt tctttctata
    22201 gcctttttag cgattgcacg aagagcacct tcttcaaatt ctagctcaac atcatctaac
    22261 tccaataact ttttatactg ttttactaga gcatttttag gttcctgtaa tatctgcatt
    22321 aatgcatctt catccaacaa ctctaaagta gctaaaactg gtattcttcc tataaactct
    22381 ggtattatac catattttaa taaatcctct ggaagtactt tttcatacag cttccctaaa
    22441 tctaattctt ttttactttc tattttagct ccaaaaccta gtgttttatc tccaccacgt
    22501 ttttgaatta tcttctctaa tccatcaaaa gctccaccta atataaataa tacattagta
    22561 gtatctatct ttaaaaattc ttgatgggga tgttttcttc ctccttgtgg tggaacattt
    22621 gcaacagtac cttctaatat ttttagaagt gcttgttgaa caccttcacc actaacatct
    22681 ctagttatag atgggttttc agattttctt gcaatcttat ctatctcatc tatatatatt
    22741 atacctcttt cagctttttc tatatcaaaa tcagcagctt gaataagttt taaaagtata
    22801 ttttcaacgt cttctccaac atatccagct tcagtcaatg atgtagcatc tgccattgca
    22861 aaaggtacat ttagagttct tgctagagtt tgagcaagta atgtctttcc agaacctgta
    22921 ggtcctaata aaagtatatt actcttttga atttctatat ccttactact agatttttta
    22981 ctgtatattc tcttataatg attataaacc gcaactgata aagctttttt agctttttct
    23041 tgacctatta cataatcatt tagaatctcc atcatttcct ttggttttgg taaagatgta
    23101 gtatcttcat ctatagtatc ttctatttct tcctgaataa tttcgtcaca aagttcaaca
    23161 cactcgtcac aaatatatac atttggacct gcaattaatc ttcttacttg atcttgattt
    23221 ttaccgcaga atgaacactt taattgtcgt ttttcttcat atttagacat attaacacct
    23281 ctctctatct acggtctctt tgtaaatact tcatctatta aaccatattc tttggcttct
    23341 agtgcactca tgaagttatc acgttcagta tccattttaa ttttttctaa tggctgacca
    23401 gttctttcag ataatatctc atttaaagtt tcttttattt ttaaaattct ctttgcatga
    23461 atttctatat ctgttgcttg accttgagct ccacctaatg gttgatgtat cattatttca
    23521 ctatttggta gtgctagtct ttttcctttt gctcctgctg ccaacaagaa tgctcccata
    23581 gaagcagcca tacctataca tatagtactt acatctggtt tgatgtattg catagtatca
    23641 tatatagcca ttccagaagt tatacttcct cctggagaat ttatatataa atgtatatct
    23701 ttgtctgggt cttcagcttc taaaaataat agctgtgcta ctataagccc tgctgtggca
    23761 tcgttaactt gatctcctaa gaagattatt ctgtctttta atagtctaga aaaaatatca
    23821 taagatcttt ctcctcttcc tgtttgttca acaactacag gtactaatgc catggtttca
    23881 tacccccttt attctatata atttacagta gcttttttaa gctactgtaa attattatac
    23941 gcatttaact aaattatatt tgcacatcat tatatactaa acatttcata acgtcaacta
    24001 tattagttag cgtttatttt attaagctat tgttgcatta tcaactaata aatctattgt
    24061 ttttcttatt tttatttgac cttttatatc ttctttgtca gcatctctta aagaagcttc
    24121 tattttttct acttccatat tgtaagcaga agccattttt tctaactcag ccttaaattc
    24181 ttcttctgtt gcttcaattc cttcagcttt agttattgct tctaatacta aagaagactt
    24241 aactaatttt ttagcatctt cttttctctc ttctcttaat tcttccatag ttctaccagt
    24301 catctctagt aattgttgta atccaaatcc ttgatattgt aattggtagt taagttcatt
    24361 taacatatta tctatttgat gttgtatcat aacttctggt atttctattt ctgtattttc
    24421 agcaactttt tctactactg aatttctagt ttctgcatct gctctatttt tagcttcttc
    24481 ttctaactta gctcttacat ctgcttttaa ttcatctaaa gaatcaaatt cactagtatc
    24541 tttagcaaat tcatcatcaa gtgcagataa ttcttttact tttacatcat ttatttttac
    24601 attaaataca actggttttc cagctaaatc ttgtgagtga tattcttctg ggaaagtaac
    24661 attaacttct acttcttcac cagctttttt acctactaat tgttcttcaa atcctggtat
    24721 aaatgtgttt gaacctataa ctaagttata attttcacct ttaccaccat caaaagctac
    24781 accattttca aaaccttcga agtctattat agctgtatct ccatcttcta aagctttgtc
    24841 ttctacactt actaatctgg catttttttc aaccatttct tgtagtttag catcaacatt
    24901 ttcgtcactt acagtattct caaccttagc tatttctata cccttgtagt ttcctaattc
    24961 aaactctggt ttaacttcta cattaagaac cataaccaac ccattatctt tacttatttc
    25021 ttctatatct aaatctggat tgtctattgg gtctatgtct aattccttga tagcttctgg
    25081 ataaacttct gggaataata tttctatagc atcattgtag aatatacctt ttccatattg
    25141 agtctctata acaattcttg gagctttacc ttttctaaat ccaggtatat tgtatttatt
    25201 tctgctttta ttataagcct tatttactgc tgcttcaaat ttattgttat ctactgttat
    25261 ttttaaagta actttattac cctctttttt aattagttct gctttcatcc ttctaatcct
    25321 cctagtgata tttatgtaat acccgataac ggtattataa ttcattctat ttataattca
    25381 accacttaat tatatcacat aagttttttt atttacaagt gttccattaa tatcgttgta
    25441 cttatttgat aaaatttaat tttttgagcg ttttttgcca tttttttaga taaatacacc
    25501 tttatatgcg tatttcttca atttttatgt catcttcttc tatatccata atgacaaaac
    25561 ttttatacga tactcctctt ggaagtgaag tgcttccagg atttatgtaa agtacaccat
    25621 ctttaatttc tctaaatggg gtatgggtat ggccaaatat aactatatca gcatcaactt
    25681 cagttgcctt tttttctaac atattagttc catattttac accatactta tctccatggc
    25741 ataagaatat ggttttattt gccacttcaa agacaacttc ttcctcaaca gcatcaaagt
    25801 cacaattacc tttaactgca ataataccta cattagtcat gctatgaata tatcttgaat
    25861 cagtaaagtt atctcctgca tgaattatta ggtcacattc tttcaaatat ggtattgttt
    25921 tttccatatt tttaatcatc atatgtgtgt cgcttactat gcctattttc attacttcac
    25981 tacccttttt ataaactctt gtttcataag ttttagtgcg tttgctctat gactgattga
    26041 attttttata acactaggta tttcaccaaa agtcttatca tatcctttta ctataaataa
    26101 gctatcatat ccaaaaccat ttttaccttt ttcttcaaat cctatcattc cttcacaaat
    26161 tcctcttact acaaattctt ttccatcagg aaaaactact gctattgcag agacgaatct
    26221 tgcatttctt ttgctcattg gtacattttg catagctttt aacagttttt ttctattttc
    26281 ttcatctgtt gcatgttctc ctgcatatct agctgaataa acaccaggtt ttttgcctaa
    26341 agcatctact tctaagccag aatcatctga tattgctata agtttagtct tttttgctat
    26401 tgctctagct tttatcaacg cattatgctc aaatgttttt ccatcttcta ctatctctat
    26461 acctgctaaa tcaacatctt ttaaagagta cactttatat tcaaaatctt ttaagatttc
    26521 acctatttct tctagcttat gagcattatt agtagcaatc actacttcat taccataatc
    26581 catacccaat atctcatctg ctatttcacc taaagcttct ctttgaagcc ttattaattc
    26641 tttattccct ttttcgccta actctaacaa taaatttaaa tctgctcttg aaaaaggttt
    26701 ctcttctcct gtcccctgta cttctacaaa ctcacactta tcagtcatta ttatattcat
    26761 atctacctgt gctttagaat cttcttcata acataaatct agcatgtgta ttccatttac
    26821 aataccaaca ctaattgcag atacaaaatt tgttattggc atctctttta ttgtctttgc
    26881 tttatataat ttatatattg cttctgcaac tgctacaaat gcccctgtta tagatgcagt
    26941 tctagttcca ccatctgcct gaatcacatc acaatctacc caaatagttc tttctcctaa
    27001 tttattcaag tctacaacag accttattgc tcttcctatt aacctctgta tttcttgtgt
    27061 tcttccatca atttttcccc tagatgcatc tcttatcttt ctttgttgtg ttgctcttgg
    27121 tagcatagaa tattcagcat ttatccatcc agtaccacta tttcttaaaa aaggaggaac
    27181 tttttcttcc actgatgcag tgcatataac ctttgtctct cccatttcta taagaacaga
    27241 accttctgca tactttgtat aatctctagt tatctttaca ggtcttatct gttcgttact
    27301 tctatcataa cttctggtca ttttttatat cctttctcat attttttatt ttttataaat
    27361 ttatttttat aatttttaat tttgtcaaat attaattatt gtcatttttc gtaatcttat
    27421 tagcttactg aactattcta tcattaatta tagtagtttt ctactaaatt aagcgacttt
    27481 ttaactctct aatacagctt ttacttcatc tttcgatgat aactttaata attctaaaaa
    27541 tctctcttca tctataactt taattcctaa atcatttgct ttagtaagtt tagaaccagc
    27601 ttcttcacct gccaaaacaa atgttgtcga tttactaaca ctagatgtag cttttccacc
    27661 tcttttttct atcatatctt tcgcatcatt tctttttaat gtaggaagtg taccagtcaa
    27721 tacgattttc atcccttcaa atattttttc tactttttca cctgtattat ccaatgattt
    27781 tgtatttact cctacatttt ttaacttgtt tataacgttc atatttttgt cttccttaaa
    27841 aaactcgaca acgctgtttg ccattatact tccaaattcc tctaattctt ctagttgctc
    27901 actagttgca gaaattactt catctaaatc cttaaagtta tatgcaagta tcttagctgc
    27961 tttaactcct ataaatttaa tacctaatcc atttataagt ctccataaat cattttcttt
    28021 tgatttttca attgaattta ttaaattgtc agcagatttt tcgcccattc tctctaggtc
    28081 gactacatct tcctttttta tataatataa atcagataca tcttgaataa ttttttcatt
    28141 taatagcagt gtaattatag attctcccaa tccatctata ttcattgcat cccttgacac
    28201 aaagtgtata attccccttc ttatttgggc tgggcaagac atattgatac attttacagc
    28261 agcctctcct tctaatctta ttgttggctc tgaacaaact ggacactttt ctggcatttt
    28321 aaaatctatt tcatctccag tacgttcctc ttttataact tctaaaactt gaggtattat
    28381 gtcccctgct ttttgaacta atactgtatc atttattttt atatcttttt ctcttatata
    28441 atcttcattg tgaagtgtag ctctacttac agtagtacct gcaagtctga ctggttcaag
    28501 aattgctgaa ggtgttattg taccagttct cccaacttct actattatat ctactatttt
    28561 agttttcttc ttttctgctg ggaatttata tgcaattgcc catctaggac ttttggctgt
    28621 atatcccatt tcgtctcttt gttttatatt gtttactttt attaccatac catctatttc
    28681 gtagtctaaa ttacccctgt tatctgtcca gtattcaata tgctcaataa cttcatctat
    28741 acttttacaa actttataac tctgacttac tgaaaatcct agtttttcta agtactctag
    28801 tgattcactg tgactttcta aatcatattc ttctatattt tccatattaa atacaaatat
    28861 atctaatggt ctctttgctg ttagttttgg gtctaattgt ctcaatgaac cagctgcaag
    28921 attccttgga ttagcaaata atggctgttc ttgttcttct tgttgttcat ttacttttct
    28981 aaaattatct tttgatatat atacttctcc tctaactatt agttcttttt tatcatttat
    29041 cttaagaggt atgcttttta ctgtcattaa gttttgagaa atatcttctc ctacaactcc
    29101 atctcctcta gttgctcctt tttcaaactc tccattgtta taagttagac ctacagaaag
    29161 accatctatt ttaaattcaa caacatattc aacattttct cccacatatt cttgaactct
    29221 cttatcaaaa tctctcaaat cttgctctga aaaagcattt gataaactca acataggagt
    29281 tttatgtaca atttgattga atttatctaa tggcttacct cctaccctat ttgttggaga
    29341 atcaattctc tttagttctg gatgttcttc ttctaaagaa attaactcct tcattaactt
    29401 atcgtattca taatctgata tttctgggct atcttgatta taataccttt cattatggta
    29461 gtttattgtc tctataagtt tattaatttc atcttttacg ctcacttttt gcacctcttc
    29521 atatttttaa aattaaatta ttttgaaatt tccctataat taattatctc ctatttatat
    29581 attttttagt atatcacttt tttgacttta aatttattac aaaaaaaact cttattagta
    29641 tctttaatac gactaataag agtttttatt tttatcaaaa ttttttattt ttaatagcct
    29701 cttgatatgt ctacaacatt atctacaggt ttattctcca ttatatagtg tttcaaatta
    29761 ttataaacca tattaaatgt tctctctttg tttttatctg acacccaaga attatgagga
    29821 gttactatga cattttcgca ctcccacaac ttattgtcct tgtttaaagg ttcactttca
    29881 aatacgtcta gtgctacccc tctaaatttc tctacacatt cttctaaatc tttctgatta
    29941 actgtatttc ctctacctac atttataaat acagaaccat ctttcataag tttaaacata
    30001 tctttgttta ctattccatc tgtacttttt gtagaaggca ttgctacaac tactatatca
    30061 cattcttcaa atacaatatt catatcttca gtagcaaaac acttatcaaa gtatttttta
    30121 ctacttccat cagtattgac accccatatt tctacaccaa aagcttttaa tcgttttgct
    30181 gcttctgaag atatagtacc tgttcctaaa aatccaactc tttgtccata caattctgat
    30241 acagaaaaat ccatcttcca ttctttattt tgttgttgtt taaaaaattt tgcactattt
    30301 ttatatattt ctaaaatata caatacaata ctttcaccaa taggtacact atatccgcct
    30361 ctattattag ctatcttaat atttctcttc aatacctctt cttttggaag ttggtctata
    30421 cctacactag ttgtctgtat atattgtaaa tttttcatct tagaaatatc taatctctca
    30481 aatggattgt atgtaactag tacatctgct gtatttacat cctcattatt ttcaatacta
    30541 tcttcagagt aatgtatgac atcatagcct aattctctta ttttatcaaa tttttctttt
    30601 ccataattta ttgtaaataa aactttcatc tcaaatctcc tctatctacc cacatttaaa
    30661 attttatatt ctaaaatata atatgatact caatgtacat tagttaaaat tactataaga
    30721 tatcttaaat ttacttatgt tcaattgttt ttcctaaaga catagcatta cgttcttgaa
    30781 ctaaattttc tattaaatta ttcaatgtcc aatcaataga tgtctctgtt accatagctg
    30841 taaaaggtct atttattctc acttttttta gaccttctaa aaatgcattt acttgtgtgc
    30901 ttggagtgtt attaaatatt acagacttac tcttaatccc attttcacat tcacttgata
    30961 tatttccatc taaaatgtct cttattatat tctctccgtt tttttcactt aagattatat
    31021 ggtctcttat tccagcaaag ttacttacgt ttttaattaa tgataattct tttttattaa
    31081 agtttacaag cataatacac ttcctattgt gtattttatc taggttatta ttatttattt
    31141 tttcaaatga cattttatcc atcctttcat ttatttatat actttaattt accccataca
    31201 tttaaaataa tcaattttta tagttaattt ttatattaat acccaaatta catttaaata
    31261 gttaggctat ccaaaactaa tatataaaat tagaataatt agattaatat ctacaaatca
    31321 taacctaaag gagagatacc atgaatacat tactaaacaa taaatttagc attaaaaact
    31381 tttcaaatgc ttgggttata gtacttggtt gtatgttaat acaagctgtt ccatttagta
    31441 ttgtagcaag tatacagcct caatttataa gttatgttgt agaaggagaa ggctttagtt
    31501 tagcttcttt ttctctaata ttcactattg gtactattgc ctcagcaatt gcatcacctt
    31561 ttataggtgt attattttct aaagtcaata ttaagatact atatttatta ggttgtataa
    31621 tttctagtat tgcttttgct actttttcta tatgtactga actatggcaa ttttacactg
    31681 tatcatgtat tgttcaaatt ggcgctacaa ttatatctgc tataggaata cctgttttaa
    31741 taagtgcttg gtttgatgaa gaaagcaaag gaaaagcttt gggctttgct tttgctggtg
    31801 gttctattgg tagtatattt ctacaaccac ttgcagttcg cttattagca actcaaggat
    31861 acagtcattc ttatctaata tttggaatta tagcattaat agttggtata cctgtaatat
    31921 tattcatact caggatgcct aaagatatta atgaaaaggt aaaatctaaa ggtaaaaaag
    31981 agaaagcatc tgatacaatt tctccttcag tatggggtta tacattaaaa gaagctaaaa
    32041 aaattaaata tttttggatt atgactattg gatttatatt tgttggtata tatgtttcat
    32101 cattatccgt acaatatcca acttatctta gaaattatct aaatcctatt ctaataggaa
    32161 cagtaggctc cacatttgct ttatttgctc tcattggtaa tttatttgga ggattattgt
    32221 ttgataaatt cggtcaaaca aagccactta ttatatcttt tgtattggtt cttatagcaa
    32281 ctttatcatt actattggta gataaatctt ctacttttgc atttgtattt gccattttga
    32341 aaggtctttc tgtatttgct tatatgattg gtcctgctta tttaactggc tctttctttg
    32401 gtcaaaagga gtttggtagc atatatggaa tcgtacaatt aatttttgca gtgggatttg
    32461 cattaggttc aacattattt ggattgttag ttgataattt tggatacaga ttttcttggt
    32521 ctattataat tttatcagtt ataattgcct atactttact tattataact tctataggaa
    32581 tgaaaaaatt gaataaagaa agattagata aactaatgca ataattgatt tttagatata
    32641 taaatattat aataaaaaag atggagcttt taatcataaa taaaactcca tcttttttat
    32701 attacaatat agctaatttt ctcttatttt taactttact atctatatcc tcatccttaa
    32761 ctatagtaaa tgccttagtt ggacaaacat ttacacatgc aggcccttct ggcgattcta
    32821 tacaaaaatc acacttattt gctgtcattc tttctctaaa gcaaagcttt ccttcatcta
    32881 catcttttaa tgtaactatg tctattgcac ctataggaca tgctaacata caagtcttac
    32941 atcctataca attttcttca tttattttta ttgtgttccc aaccctaact ataccaccat
    33001 ttggacatgc ctttgcacaa ggagcatctt cacaatgtct acactgtatt ggagcactta
    33061 cttcttttgt ctttatgaca gaaagttttg gattaaaatt tatttcatct ggattttggt
    33121 aaaaaatatc ttcatctgaa tgtgcaacta cacatcctat catacaagtt ctacatccta
    33181 tgcatttatc agggtctgct ataacaaaat agttcatcac ttagccactt cctttcctat
    33241 acaatatgta gtgtgtaata gatgatgaga tttttctcct aatggttcta ccagatattc
    33301 atcatacagt tttttaatat caggattctc atgagatttt ctaattgcta aatgttcatc
    33361 atgcacgtat gttgcttttg ttctattttc ataagccaat tccttatatt cttctagtaa
    33421 aatttttggt tgacctccac cacttacaca cccaacagga caagtcataa cttcaataaa
    33481 atctacatct aattttcctt tttccaagtc ttcaagcact ggtattacat tttttaatcc
    33541 acttactact ccaactctta agtctaagtc tcctatttta atagttgact ttctaaaacc
    33601 attttctcct cttacttgtt ttacttctac atcaggaata ctttctccag ttataagctc
    33661 atatcctgtt cttatagcag cttccataac cccaccagta acaccaaaga tagttccagc
    33721 tccactataa ctacctaaag gactatcaaa tttttcttct cttaaatttt taaaatcaat
    33781 tcccatgtct tttattaaat aagctagttc tctagtagtt aatactacat ctacatctct
    33841 atatccacta gaatccattt cttctctatc acattcatat tttttacatg tacatggcat
    33901 tatagaaaca ctaaatatct ttgatgcatc tacattattt atctctgcac cataagtttt
    33961 aaatattgca ccacccatct gctgaggtga tttacaagaa gataaatgat tagttaattt
    34021 aggataattt ttttccatga attttaccca agctggacaa caagatgtaa acattggaag
    34081 agttccacct tctgtcactc ttttaattaa ttcactacct tcttccatta tagttaaatc
    34141 tgctgcaaaa ttagtatcgt agactctatc aaaatttaaa gctctaagtg ctgctgccat
    34201 ttttccagca cctaagtatc ctaaataata tccgaaatct tctcctaaag caactctaac
    34261 tgctggtgca cattggacca tagtgaatac atttgggtca cttaaagctt tctttacctt
    34321 atctatatca catacattgt atgctgcaaa tataggttca tttatagact ctggtatctc
    34381 tctttcttgt tttttctctt gtaaaaactc aaatccttca tctataactg aggcataaga
    34441 tttacaagtt tgaacacatt gaccgcacat aacacatcta tctaaattta tctcttgtgg
    34501 ctttccctct tcaccttcaa tagcatttac tggacagacc tttgaacatt ctctacatcc
    34561 agtacataaa tccttatcta tatttattat cataaacgtc acctacccct gtatccttta
    34621 tttatttgtt aacaataaac taagtgcagc ttgtctattc ttagcttttt tgtcatctat
    34681 tggagtaaca agttttaatg cttcctgagg acatgcactt atacaagcta ttttttcatt
    34741 gtctttacat aagtcacatt tataagctat cttcttattt tcatctaaaa ttacttgttc
    34801 tacttcttta ccatcttcat attgtggaag taagtctata gctccaaaag gacatgctag
    34861 cagacaagtt ttacatccta tacacttttc ttcatttaca gacataatat tgttttcttt
    34921 gactatagct ttttgtggac atgagtttaa acaaggagca tcttcacaat gtctacactg
    34981 tattggcata cagaaattgt caccttttac taaataaagt ctaggtataa caggtatatc
    35041 aactgttcca actgtataac ccacattatt atgctgatta tgcattgtaa aacaagctac
    35101 ttcacaagct ctacaacctg tacatttgtt tgggtccgct attacaaaag agcctatttt
    35161 atttttcata ttatcccacc tttcaatttg tatatttcca accctctagt tactttcatc
    35221 atatgtctac ttactttcat catatatatt tatatatttc actattttct cagtacttat
    35281 atttcgttta cattttttgc acaaattgta tgtgttatta tacaccttat cagtatcgtc
    35341 aattaatact ccacagattt cacactttga acatttttgt tcttcttttt tagtacacat
    35401 atacttattc tcactttttg ataactctct gtggatttgc ataaatattc atccgtcttc
    35461 ctcttacaaa tccaataagc gttatattta attttttaga aagctctatt gataaattag
    35521 tcggtgctga ttttgatact acaataggaa tttgagtcat agctgctttt aatatcattt
    35581 ccaatgatat tcttccactt acagccacaa ttttatcttt taatgttatg tcatttaaaa
    35641 cacaaaatcc aatagcctta tccattgcat tatgccttgc tacatcttca caagtgacta
    35701 tttttttatc aaaattaaat acagatacgc tatgtacccc accagtattt ttaaataatt
    35761 ctgatgaatt taaatttcta tccattatac tgtaaatcgt atcataattt atttttatat
    35821 tgctctttac agaagcacat gttatatagt ctagtgaatt taaaaatata atttgctcat
    35881 cttgattttt ttgtttctct tgaatagtta cttttgcaaa acgttcactc tcatttatct
    35941 caaattttaa taagcttttt ttgttttcta tgtatccttt tgttttcaag aatcctacta
    36001 taagttcctc taaattatat ggggtacata aaaatgtatt tacatattta tcatttaaaa
    36061 taaaactaag tggatattct gcaattattt cttcattatc tattgatact tcatgttcat
    36121 ttattttgct tacttctaca ttaaaagaat ttaaatattt gtagtccact agactataac
    36181 tattttcaaa aactttatcc ttactatttt cttgtaattt agtttttgag tatatattgt
    36241 tcataaaata ttagcctttc tgccttttat aaatcttaaa tctaataaat cattagatat
    36301 ttgtagctgt cattttgtct ttcaaacttt gatactcttc tcttacacat ttttctgcta
    36361 attcttggtc ttcaagtttt tcaagtttaa cagcacaata tttatactct ggtgttttag
    36421 atatagggtc taaatttgct attgtaagtt cattgcaagc tccaacccac cactgataag
    36481 tcatatatgt tgcaccttcc ttaactctat cagtaactgt tgctcttgtt attacacttc
    36541 ctcttcttga actaattcta actaattcat catcttcaat tcctaatttt tcagcatcat
    36601 tagaatttat ttgaacacgt cctggttcat cttctaatga actaagtgtt ctacaattac
    36661 cagtcattgt tcttactgaa tagtgtccaa cttctcttac agtacataaa ctaaatgggt
    36721 attcatcatc ttcaacttcc atagggggtc tccattcagc agcgaacaaa ttacctttac
    36781 cattaggagt agaaaacttt tgaccttcat acagatacat agttccttta tcttccatac
    36841 tttcactctt acaaggccat tgaacgcatc cttgagtttc tatcttttca taagtagcac
    36901 ctaagaaact tggacataat tgtctaagtt cgtcccatat ttccttagta tttttataat
    36961 tcataggata tcccattgct gtagaaattt cgcttataat ttgccagtct ggttttacat
    37021 ctccttgtgg ctctatagct tttctcatca attggaatcc tctatctgca catgtatata
    37081 ctccctcatg ttctccccaa gaagtagctg gaagtataac atcagcgtga agtgctgtct
    37141 tattcataaa tatatcttga acaatgacaa attccaattc atctaatgct tctctaactt
    37201 ctgatgcatc tgggtcactt tgcactgggt cttctccaaa tatataataa gctttaagct
    37261 ttttctcttt tagtactaag tctggaactt gagttaaact ataaccatta ttaggagata
    37321 attttacccc ccaagttttt tcaaactttt ctcttatctt atcatcagtt acattttgat
    37381 agccaggata tacattagga agagctccca tatcacaagc tccttgaaca ttattttgtc
    37441 cacgtacagg tcctattcca acgctttctc taccaaaatt acctgtaagt agagcaattg
    37501 atgcaagtcc cttaactaca tcaacagctt gaccaaattg acagacaccc attccataaa
    37561 gtatcatagc ttttttccct tttgcatatt ctctcatagc ttttcttata agctcttctg
    37621 ggattcctgt aatcttttca gcatatttag ctgtatatgg tttaacaatt tctttatact
    37681 catcaaatcc ttgtgtatga ttttgaacaa attctttatt gtaaagacct tcttctatta
    37741 atacatttcc aaaagcattt actaaaacca tgtttgttcc accttttata ggtagatgta
    37801 tatcagcaat tcttgcagat tcagttactc tagggtcagt aacaattaat ttagcaccat
    37861 tttttttagc ttttactatt ctatttgcca ctattggatg tgaatctgct ccattatatc
    37921 caaatataaa taaaacatct gcattttcta tctctggtat agaattagac attgcaccac
    37981 tacctaatga gtaagccaga ccggctacag atggtgcatg tcatacacga gcacaatggt
    38041 caacattatt agttcctatt gtagctctca taaatttttg cattatatag tttgcttcat
    38101 taccaggtcc tcttgcagaa cctgttccca taatagaatc aggtccatat ttttctttaa
    38161 tttcatttag tcttgaagct gtatagttta tagcctcatc ccattcaacc tcttctagta
    38221 caccttgttt tcttatcatt ggctttttta gcctaggtgt tagaatttta gggtcattta
    38281 gaaaatccca tccataacga ccttttaaac acaaactttc ttgatttgtt ctaccattag
    38341 ccggttcagc tcttaatatc ttattatctt ttactactag atataattgg catccagctc
    38401 cacagtatgg acaaactgtt aaaatttttt tctccataca attcccccct tagatttatc
    38461 ccctgataag taaaatttat cttaaagata atgagtgttt ttataatatg caaaatcata
    38521 aatattttgc aatactatct attaaattca acaattttct cacaattcct cttaaatttc
    38581 aaaaaaaata tgtcataata atctttatat ttcaaccatt taattaaaat attctagttc
    38641 ttatgacata ttttttaata tactcaatat atattcttta aacttattta gttttcactt
    38701 aaaacaatat ttctgccaat tctatttttc attctatagt gagtaattac agtaaatatt
    38761 ccacttccaa tcagaattaa atagtacata aatccaccat ataaaaatac tgcatatccc
    38821 atcaaaggtt gagggaatac tgatttgaat atagtgaaaa aggctaattc attagctcct
    38881 acattcccag gtgttggtat tggagatatt gccatatata aaaatgcttg taatgtcagt
    38941 atataaatat agctataacc ttgtaaatta aatgctttat atatccaaaa tgaaacacta
    39001 aagtaagcac ttaattgaat aaaagttact attatagttg aaattaatac ctttttattc
    39061 tttgcaaaaa aatttattgc tttactataa tcatctataa aactttctat ggattcaatc
    39121 ttattatcta aaaattttaa aaacttaatt tttgataaat gctttatagt aattctcata
    39181 aaatatttta tttttttagg gtttaaaata actaatatta tcataattaa gacaaatgaa
    39241 ttaatggcta tccctagaaa cactagaggc ataatcattt tcatttgttt ttctaacata
    39301 gcaaaattca ttaatacaag tattgtacaa taaaaagtaa ccactatttg gaaaataata
    39361 gacttattgg taacaactgc acttgcctta ctaagtggca ttttacattt tttaagtaca
    39421 tatatctgta ctggttggct tcctgaagca aaaggagtta ttaaattata ataaaatccc
    39481 ataatggcta gcttaaaacc aataaatcta atattaactt tgtgagtgct ttctataatt
    39541 atcttcatta ctactccttc aagcattatg tgacacatta ttgccaaagc acctatgaat
    39601 aaaaacttct tatctaccat aaccataaca tttgatagca ttttaatatc caatgtttta
    39661 aacactaggt aaattgttat acctattaac aaaaataaaa atgtatactg tagtaaactc
    39721 tttctaatat tctttagtct atccattaga aataaccatc ctttgctcaa gatttatact
    39781 aaatactata ttattagtat acactatgaa tcttaatatt ttataacaaa ataccttaca
    39841 attttgtcat ttacaaaaaa tattaatcta taaattaaat tttaatatat agattaatat
    39901 tttacaaaca ttttgtagat atatataata ttttcactaa gcaacactta tacttattac
    39961 acccatttat accctatccc ccatacagtt tctattgtat aaatatcata ttgttttaat
    40021 ttgcttcgta tattttttat atgctctgtg atagtactta tatcactttc tccatcaaaa
    40081 ccatataccg actcatatat tctttctttt gaaaatactt gtcccttatt ttttgctaga
    40141 aattcacata ttagatattc acttttagta aaattaattt ttttaccatc aattgaaact
    40201 tctttgccta aaaagttaaa ttgaacattt gagatagtta ataaattctt tttttctcta
    40261 ctttctcgcc ttaaatgagc agttatccta gcccttaatt ctcctacacc aaaaggtttg
    40321 gttatatagt catctgcacc tatgccaaga ccgtacataa tatcactttc catatttttt
    40381 gctgtcaaaa acagtattgg acaatcaacc ttatttctta tagttttaca tacttcaaaa
    40441 ccatccatct taggcatcat aacatctaat attattagat taaaataatt aaaatccatg
    40501 ttcatagcct tttctacatc actaattacc tttacttcat gtccatctct tttcaacaca
    40561 ttttcaatca actttaacat atctaactca tcatctacta ctaatatctt agacataata
    40621 actcctccat ttatccgttt aatagtattc taatttatat ctttaataat atctttaaaa
    40681 ttcacattaa ccataatact ctcatattat agcttacaat aattaattac tattttacaa
    40741 ataaaatatt ttagatacaa aaactttata tttcaaaaac tttatattca ttttacacgc
    40801 atttacttaa atataaaaat ttatttaaca atttattatt gtaatatttt gaatatataa
    40861 taaaattaat aataagaaaa ctttttataa atacactttt aattgtttaa attggaggaa
    40921 aaaattatgg atgaaaatat aacaaaaaat aattataatg ttccatttga ctatgttcga
    40981 aaaaaactca aagaattaga ccctcataca attagtaatt taagtaatat acctttcaat
    41041 aatgatttaa atagattcac actcaatttt tttgatagac aatatattat agactatcca
    41101 agtggagaag tttggaatag taatggtagc ctttgcaata actttgagct taaaatcttg
    41161 atagttagat atttaatcaa tgctaaaggt atccctagaa ctaataaata tgtaactttt
    41221 aaagaaatac caggaggcaa tgtttattat ccaaattttt taaacaggac tctatatagg
    41281 ctttcagaag tttttacatc tcaaatagac aaatataagt tagttatgga gaaaattgga
    41341 gctgaaaaag taaacatggg agatttagct tataggttta catatatggg taatacatct
    41401 atgatattta tactttggtt tggagatgat gaatttcctc caaattcaaa tatattattt
    41461 gattctaata tagtttatta cttcaatgca gaagatttag cagttgttcc tgatacagct
    41521 ataaatgcaa ttttaaataa aataaatcaa ttataaatat aaataatatt ataataaagt
    41581 ttttgataga tttaatagaa tataatatga aatggggtaa aaaatgaatg tagaaaatca
    41641 atctttatca gatattgaaa taatattatc acacaggtat gataatggtg cagactattg
    41701 gactacacct gataagcgtt taataaaagg ttctcctttc tctactcttg atagtgtact
    41761 ttatctatta gagttaggtg tgaaccctga tgatacttta cttaaagaat gtgctgactt
    41821 gatatttagc acatggcgag aagatggacg tttcaaacta tatccaaatg gaggtattta
    41881 cccttgtcat actgcaaatg ctgctaacgt actctgtcat atgggatatg catctgacat
    41941 tagaatgcaa aaaacgtttc aacatttttt agatacacag tatactgatg gtgggtggcg
    42001 ttgcaataaa tttagttttg gtcgaggtcc agaaactgaa tactcaaatc cactaccaac
    42061 tcttaatata ttaaatgcat tccgttttag taattatcta aataaagaat caaaacttga
    42121 caaagctgta gattttctac ttgaccattg gactattaaa aaaccactcg gcccttgtca
    42181 ctatggaatt ggcacattat ttatgcaagt tgaatatcct ttccgtaatt ataacctatt
    42241 cctttatgtc tacgttttat cattttataa tcgtgcaaaa aaggataaga gatttttaga
    42301 agcattgaag atacttgaat ctaaaatgat aggtaataaa attgtcgttg aacgtgtagt
    42361 tccaaaacta tctaaattat ctttttgtaa aaaaggcgag tcaagtgaaa tagccacaac
    42421 acactattac gaaatattaa aaaacctaga aaaataatag ttatatagat aaaacatatt
    42481 aaaaaatagt ggctttaaat tttttaaagt cactattcta tataaatata acattataaa
    42541 aaatatatta aactatttac tcttttttaa aaccgttgat tttcttgcta tataaaaaac
    42601 tattattata gaaataacta ctagagaacc tctatttata aagaaataat tctttaattc
    42661 attaaatcta acatctatat tattaggcat ataaaacatc ttttgaatat aaaatccatt
    42721 tacattaatc atactaacaa atgggtacat atgtaaaata aaaaatatat cagcaaatgt
    42781 atatgtaatt aatattgcta ttgttgtaaa tctatttttt gttaatgtta atatcattag
    42841 tggaaatgtt gtataccata ataatgtagg aaatgtcttt aaaaacacat ctattggctt
    42901 tacactataa acaaaatcta ttttgtctat gttaaaacct ttcactgacg taaatgtata
    42961 tactattgta attgaaatta ataataatac agtatatata gctaatataa cacaccatct
    43021 aaatactata aattgattat attttttttt attatataaa gtaagtatct catttatatt
    43081 acccaaataa tcttttgaaa acacataaat tattgtgatt gctgacatta ttggtataaa
    43141 aacatctgat atctttataa ttcctaacca tcctgctata gaatatgtat ccattatagg
    43201 tacatcatta taaaatatta actcattata atctaatatg cctgtaataa atatgcataa
    43261 tatagaaata attacaaata ttaacacatg tttaagctct atttttttaa gctcaaattt
    43321 tattaactcc aatatacaca tttattttaa ctccttatgt aaaaagttta tataagcatc
    43381 ttgtaaagtt gtatctatcc ttaagctatc cttaagtttc tctgtatttt tacctatata
    43441 ctttacttgc acaccatctt taactcttct ataatcaata atatcaatat tacgttctaa
    43501 atattcaaat tcatcattag aaactactac tgaatacacc tcaaccatat caataaattt
    43561 attaattgta ccatcatata atattttgcc ttgatttaat ataattaatt tgtcacagtt
    43621 aaattgtatg tcttctacta tgtgagttga aattaatact atagaatttt tacttatttc
    43681 tggtaacaat cttctaaatt cattcctttg ctctggatca agaccaactg taggttcatc
    43741 cataactatt aacttagaat cacctattaa agcctgtgct atacctagtc gtctctttac
    43801 accacctgag tatgtaccta ttttattatt tataaacttg tccagattaa cttttgatac
    43861 tactttttgt atatgtttct ttaaatcatt cttagataca cctctcattt tagctacaaa
    43921 ctctaagtat tcttgcccat ttatattatt atacatatca aaatcttgag gtatatatcc
    43981 tacatttgcc ttgattttct cattgttttt gctatacttt atattattta attctatctc
    44041 tccaattgaa gtttcataaa gagtcactaa taacctcatt aatgttgatt ttccagcacc
    44101 attaggacct aataatgcaa ctactccatc tgttagggtt aaatttatat tatccagagc
    44161 ttttttattt ttgtaattaa aatttagatt tcttatttct aacttgttta tcatattaac
    44221 ctcctttatt atttatctat tataaggata tttcctgaaa gccaatgata aatagttatg
    44281 ctaattttct ctccctttga gtcttcatat aaaaattcat tgctaggtaa gtcagcaact
    44341 aatatcatat taattttttt tctttttatt gattccataa cattatctac ttcatctaca
    44401 tattttttag catctacaga tgctttgcta tcacctaata tttcatttct tgtttcaatt
    44461 aaaaatttaa ttttttcctc tatatccata tttacattac tatctgatgg atagattatt
    44521 tttatatcat cttttacctc ttctttaaac tcacctgcat atattgcaat atcatttgac
    44581 tttccttcaa atatataatc tttttttaaa aaattactag tcttattcag taaaggtaaa
    44641 tttgaataga cattattttt agaacttaaa tttattttat aagccataca acttgattga
    44701 tttacttttg ggtaccacgc taaagaacct ggtggaagca taatatcatc tttagtagct
    44761 atatatttat tttgattcca agatgtcaac acattaatat agccttcata ctctatatct
    44821 aatgtcacct cgtcattagg ctctaaactt tcctttaaat taacttcaat taaatccttt
    44881 tttttgttaa attttatatt ttccgaattt acttttatat attttatatc aaatatttca
    44941 tctaaaatca tattaatatt tgttaactct tcatttgatg tgtttaataa aacaattcta
    45001 tcttttatat ttgacttgct tttaaagtca atcttcatat catgcgattt tattttaata
    45061 tcactaatat tagatttctt taaatactct ccataactac gttcctcatc tctatatgtt
    45121 gacatatcta ttgatatata atttaatcca ataaataaaa tactgtacac tactataact
    45181 attaatgatt taatctgttt ctttctaata tcatttaata tcagatatgc tactattatt
    45241 aaagtaattg caaatagtgt aggtataatc ttatctataa catatgctga attattaact
    45301 ttccctacaa aatcagaata gtaagcatat gattgatctt caaatatatt aagtgattta
    45361 aataatgctt taaaaaatgg ttctttaata tcaataactc tatccaacat cttagttgat
    45421 gttaaatata aaaatactga tgataaggta atctttagta ttaaattatt tataataact
    45481 gaaataaatg atatcactat agataaaata gtaagtggta aaaaccattc aaataccaat
    45541 ataaatatag ttgatttaat cgttacaatt gataatgttc ttattaaact tgagtatgct
    45601 atagcatata taattggtat gcaacaaaat ataaagttgt ttctagttac agcaacagaa
    45661 atacctaaaa aataatcaaa attatttcta cataatattt ttacagtatc ataacttttt
    45721 ctcatataaa aaattacatt taacatggtt aaaatactaa ttgtcataca agtatataca
    45781 ctcaaggata agtaatcatt aaatgaaatt cctctattgg atatatgtgt ccatcgttgt
    45841 atgagatggt aatttataat aataactgtt attgcaaata aaatattttt tctaattatt
    45901 ttggttataa agtctgatat aaccttaata ctactcatat ttatgcccct cctagccaat
    45961 cctttgatac aacttacaat agattcaact atatttaata agttcaaata tttaccttga
    46021 cattattttt taaaattaat attaattagt gaaattaatt aatttagaaa atgttttata
    46081 atcttactga tcattattaa ccttcattcc aatatatatt aatatagcaa tccaatatat
    46141 agaaattatt gcataaatca catattgctg aaaaatatta agtattttac ataaaataat
    46201 taaacatatt attacgaata gtattttttg cacagtcttt ctataatgct ttttctcact
    46261 ttcactaaga ggtttgtttc tatgttctaa aggagctaac ttgtaaattg ttagtatact
    46321 aataatcatt aataatacta ataaatactt atatttattt atatctatat tattagctga
    46381 aaaaatagtt atcaaataaa taactgcaaa tgttaatgta cactctttat aattcctagc
    46441 atgataacct cctgtgaact gtcttaaagt gcaataacaa cttaaaaata taactgtatg
    46501 tataaatcta tcaaaaataa ttcctataaa taatgctacc gaaatattaa ctatgaaata
    46561 aatcaaagtt tcaaatccat atctatatat ttcaaaatct tcactttcta caatctcatt
    46621 attaactaat atattggcaa atttatatga taatctttta aacatatttc ctcctcaaca
    46681 tgtaaaacct ctcctttaat aagaagaggt ccttttatta atgttatttt tttagttttt
    46741 gtaaagcttg tggctctttt gcctgatgtg ctacccaaga acttgcactg tttgctgata
    46801 atacagctat cccaaaactt aatgctgaga tgtttttaag taagtttaac gctatttttt
    46861 tcattttgta ctctcctaac tttttattgg tatcattatt tttaatataa actcattatc
    46921 tgagtaatta actattgttt caccaccata tttatttact atatatttaa tactagcaag
    46981 tcctattcca tgtatcttat catcatcctt actagtttga atacgtttat caataagttt
    47041 aatatcattt accttagtat ttataaattt tattattgca aatttattta tatatgtagc
    47101 tttaacttct attctttttt ctagctcatt atttatatcc atacatgctt caattgcatt
    47161 atctagtgaa tttgcaaata ttgaacatat atcattattt tcaataaagt ttaatttaga
    47221 tatatttatg ttatcttcaa tttgaatatc atacttttta caaatactta ttttttctgc
    47281 taaaattaaa tctaatgttt tatttccagt atttttaaaa tgttcaaatt cacttatctg
    47341 aaactctaag ttatctatat atgatataat ttcttcttta gtgtcataat tttttataca
    47401 aatcatatgg tttttcaaat catgataaac atattttaat ctatcatgaa cttcgtttat
    47461 attttcataa ttcctgtagt ttgtttttat tctttcattt ataagttcat actctagatt
    47521 taatttatcg tcttgtacaa ttttccctat tactattaaa agtattatac ttgatgataa
    47581 aatcaataaa attgcaaata taaatataaa taagttttct gtagtaattg tattgactct
    47641 taaattatat tcaaatataa gtaataagct aactatatta gataatatag gtattcctat
    47701 tagtatcata tctttaggtt taaaatctaa agataattta aaatacttaa acaatataaa
    47761 acctataaat aaaaatacct ttgacataat aatagcttgt attctaaata agttttcatg
    47821 ttgtgttatg ttaatattat ttaatttatt tataaatact actagaccta ctgcagaacc
    47881 ttcagcaatc attaatccta gccaaaacaa taaactaact attatacttt tatatatttt
    47941 atcttcatat gatgttatat aaaatgatat tcctattacc atacatagaa gaatcttaat
    48001 atttggaaac actttaacta catttatgta tgccataaat ataaatataa ttattgttgt
    48061 aaatatatat cttatattag atattttcct ctttccaact aagtggatta ctaaaaaaaa
    48121 tataatccaa tctatagtac ctgaaactaa attaataata ttccaaaatc ctatacttag
    48181 atttgttagc atactatatc ccctaatata ttagttatag caagtttcaa gcctttaact
    48241 ctatatttac ttattggaat acttttgcca tctactataa cagtacttcc actcattgat
    48301 tctactaatt ttaaattaac tatatagcta ttatgacatc taaaaaatcc atattcattt
    48361 aatatctttt ctattttaga tatactcatt ttagtaatat acatatcatc atgtgtataa
    48421 atcaatatat tcggtctatc tgtttctata taagtaatag aatctatttt aattcggtct
    48481 acataatttt ttacatttat agttaagtaa ttatttctct ttttcatcat ctcatttata
    48541 caaggtaata cacttttaga aattttttct ttatttattg gctttagtat atatctgtat
    48601 gcttttacct cataaccttc ttgcataaac tctacaaatg aagtaacaaa tattatttct
    48661 aacttatgat caaactctct tatcttccta gcagtatcca taccatttat tgttttcatt
    48721 tgtatgtcca taattaatat gtctaaatct ttaggatagt tatttagtaa ctcttctcct
    48781 gatgaaaatt catatatgtt ataatttata ggatatgaac ttaatatttc acttaatata
    48841 tcttttattt ttattcgata atgtagttca tcatcacata ttcctatact aatcacaatt
    48901 cttaaacatc ctttcttata tatttttaat aaatatataa aatttctatt cattttacat
    48961 attttaacat acttctgtag caaatagcta tttagatttt aattttagct ttttggacat
    49021 taagtattac tttttggatt tacaatctat tttaattttc attataatac aattttttaa
    49081 tatatattga atatatttag cgattttaat aacatattct tcgagaagtt tttagtatct
    49141 agtttttgtt aaacaaaaag tatgcacctt cctttttata gtaatatttt aattatcgct
    49201 ataattttga agaatacaca ctatatttta aatattcata taaaacatat taaaaaatag
    49261 tggctttaaa ttttttaaag ccactatttt tttaaatgat gttcctgaaa atatattaag
    49321 ttagttattc taatcaattt gaagactttt gcttctcctt gcctatatct aacataattt
    49381 cataatttaa tttacctttc tttaaatcaa ctcctgagtt acttctaata atcccacaat
    49441 ccaacatttc gtcaaatacc ttcctagata aacctagttt ctcacgtaat aatgttgata
    49501 cctttaactg acatggatat ttactaataa tatgaacttg tgtaaggtca ttaatattaa
    49561 tttcttgtcc tactatttta tactctggaa taccaacttc tgctccattt ctatgtaaaa
    49621 gttccgtatt catagcatat tgcttagcaa gattataatc gtttgtatgg aattgttcta
    49681 gtgtttctgg atttaagctt tttggattga cacgagaata tatagttgag ttccatgttg
    49741 aatcacaatt tgaacacttg tatattaacc atatatccaa atatttatgt tgagcattaa
    49801 ccctaaataa acctgaacaa atatattcag tcttttttcc acatttctta caatacctaa
    49861 ttacaggtaa ctgggagata tattccacct cccaagtaat ttttttcata aatgcaactt
    49921 cctttctata agattactaa attaatagaa agttatgaaa aggtggaata taatcaagta
    49981 tttttttcaa aacaagacct cctaatttta ataaatttta atttcaaaca tataaaaagc
    50041 tactacttct tattagatac tttacaactc tattcgttat ctgctgtaca agttattgaa
    50101 attatttaaa ttatagcatc cacttataag aaactatacc tcaaacactt ttatcaaaag
    50161 aaaatgagcc agtataatta taaaactata ctggctcatt cttaactatc ttttcttttt
    50221 tcttgtaata caattctttg aatacttttc aaagacaagt aatatttttc tgataaatat
    50281 ttcaaatcat atccaacttg ataatcttca taaatttgca tatttctaat tgataaatct
    50341 tcacgagtag acgtactact tccccattct tttttattat tagactttct aggaatatag
    50401 atatattctc catctacata ttcctgtatc aattcaagta attcttctgg taatatatgt
    50461 attgcttttt tatagctcat tctgcactcc tattaaataa taatttttta ttaggaatag
    50521 caaagggcta tttatatatt aaattttata tttgcaatag cccttgctat gcaaaaataa
    50581 agattaaatc aaacataaat ctcacccctc cttatatatt gcaacgatac atataagtct
    50641 atcagcagta taaatagttg tcaattaaca taacttcttc atctaacctt tatattatct
    50701 ttatttattt tcataattca tctattttag agactaactt ttttcttctt attagatata
    50761 tttaaagtta ttaatcaaca taagtccttt atcttgaggg atttattacc gaacaaggaa
    50821 ccccaactat acactttcca caaatatcac aaccacctat ataagtgtat tttttttcat
    50881 ttatcataca catttcataa cacttattcc tattaaatcc atcttctttt aaagcttctt
    50941 tcacgcattt gttgacacat tttttgcaac tattattatg tttatacaag cagtattcat
    51001 tttctggtct tttagttggc tctaattcta aacttgtgat tatgcttcct attcttccac
    51061 aacacccttt ttctgtaata agcatattat ttaaaccaaa tgttccaaga cctgctatga
    51121 atgctacatg tctttgtgac cagtcactta ccaatttttc actgtcaaaa ttatacgacc
    51181 caggaatagc atatgaatta gcccccatct tctctagttc ttcacataag aaatcattta
    51241 aatttgaaat caacatatta gtttctatgt acgctcttgc ccatatttca gaacattcaa
    51301 accctccaat attactatta tttacacttt catcaaaagg tataaaatat gtaattatag
    51361 tttttccatt ttccagaaaa tcttttggca tagcatgaga tgttcctaca acacttttta
    51421 gcttctgaaa catatcatct tcagcagatg cataagcaaa caaaagttct tgccactttg
    51481 tagttatatt atttgattta tgataatttt ttacaaagtt gttcattgat gtttcaatct
    51541 tatttttcat agtatcaccc ctagataatt ctatatttcc aatttaatgt tattatttta
    51601 tattatataa tagtatttta taatatgcgc actaattaat attaaaacta tgttcatcaa
    51661 tcattcttaa aaaagctcct aatatcaata cgatattaga agccttttct tatctaatct
    51721 aatttattgc aattcctgca cttgcatatc ctgctacagt attaactaat gaaagacctg
    51781 aagctgttgc agtaaatact agtaagaatc gagtttctgc tgttgctgct atatttaatc
    51841 ctgttacaat tccactagaa attgaaccaa ctgataatat acctgtaagt ggtggagcta
    51901 gtgtaactgt cgctcctggt acagctgtaa atgagttatt tggtgcagta gattggtaaa
    51961 gtgttgctgt aattgtaatt gttgaaccaa caagtgaaag tgctgctgtt gtactgaagt
    52021 atgctgaaat agatgttatt gttccatctc ttggcattga aaatgcaaag ttagtcaatg
    52081 tccctgctgc gtttgtaagg tctattactc caccaactat acttaatcct ggagctgaac
    52141 taccaaagcc aacaaaacct ggtgtaccta ctaatcctcc agctatagtt gtaagtgata
    52201 gtggtatacc tgatgcaaaa ggtattattg cactagcacc tgttgggcct gttggacctg
    52261 ttgcccctgt tgctcccgtt gctccggttg gacctactaa tccatctgcc cctgttggac
    52321 ctgttgctcc ggttgcccct gttgctccat ctgctcctgt tggacctgtt gctcctattg
    52381 ggcctgttgc tccggttgct cctgctaatc ctgttgctcc ggttgctcct gttggaccta
    52441 ctaatccgtc tgctcctgtt ggacctgttg cccctgttgg acctgttgct ccatctgctc
    52501 ctgtatttcc tgttgcccct gttgctccgg ttggacctgc tactccatct gctcctgttg
    52561 ctcccgtcgc tccggttgga cctactaatc catctgctcc tgttggacct gtcgctcctg
    52621 ttgctccggt tgctccagtt attcctactc cggttgcccc tgttggacct gttgggccta
    52681 ctaatccatc tgctcctgtt gcccctgttg ctcctgttgg acctactaat ccatctgctc
    52741 ctgttggacc tgttgctccg gttgcccctg tcgctcctgt tgctccatct gctccggtta
    52801 ctcctgttgc cccggttggt cctattagtc ctgctgctcc tgttgcccct gttactcctg
    52861 ttgggcctgt tgctccggtt gctccggttg ctccggttgg acctattaac cctgttgctc
    52921 ctgttattcc tattcctgtt gctcctgttg cccctgttgg gcctgttatt cctattcctg
    52981 ttgctcctgt tgctcctgtt gggcctgtta ctccatctgc tcctgttgcc cctgttgggc
    53041 ctgttgggcc tgttactcct gttgctccat ctgcccctgt tgctcctgtt gctcctgttg
    53101 gacctgttat tcctattcct gttgctccta ttgcccctgt tggacctgtt gctccaggtg
    53161 ttcctgtcgc tcctgttgga cctattaatc ctgttgctcc tgttactcct actcctgttg
    53221 ctcctgttgg gcctgttgga cctgttactc caaatcctgt tgcccctgtt gctcctgttg
    53281 gacctgttat tccatctgct cctgttgctc ccgttggacc tgttggacct gttactccat
    53341 ctgctcctgt tgctcctgtc gctcctgttg ggcctgttgg gcctgttact ccaaacccta
    53401 tagctcctgt cgctcctgtt gggcctgttc cccctgttgc tcctgttgga cctcttaatc
    53461 ctgttgctcc tgtattccct gtatttcctg ttgggccagt cggaccggtt ggtcctgttg
    53521 ggcctgttcc ccctactcct ggacctgttg gacccgttgg tcctgttggg ccggttctac
    53581 ctcttggacc cattgggcct gttggaccta cacatgatgg tggacagcaa tgatggcaat
    53641 cacagctatt ataatcatca cgaccattat tacaatcatc atatttatca tagccattgt
    53701 tgtaatcatt atcatcaaat ggtccaaaat atttatttct actcattatt aaaagcacct
    53761 cctgatatat ttgagctttt aagctctata tataatatgt caaatttagt atgattgtga
    53821 cctatcaact atttttaaat ttactaaaat aattttcatt gtgcaaaaca gcactatcgt
    53881 taggtttgat ttcttttgtt ttttcatgat aataaatagc tttatctgat tctcctaacc
    53941 tgtcataaca gacacttaat tgtatagatg gtatatatcc ataacaatca tttgacttaa
    54001 aacctccatt agtatcattc ttatctcttg ttaatgctac cttataccaa aaaatggctt
    54061 ctttatattt ttgtctatca aacatatgct ttcctatgtc acaacaaatt tctgctcttg
    54121 gctcatcaaa ttcgaaactt ctaaacaatg aatataaggc acttttttca tcattaatta
    54181 agtaataaca tgtggctaaa tctctgcagg cactgataca atcctcaatc catcctcttg
    54241 atgagtctaa aaacttagta aaaccttcta ttgcttcttt atacctagca ttatagtaca
    54301 actctctaga gtaatagaat tggtgtcttg gctccaatgt ttttccttct gaaatcattt
    54361 tttcaaaaat tctaagattt cttagagggt cctgtctatg aagcttttta tgagatattg
    54421 caatgtcaga ataaaatacc tctccaaaaa gaggtactac ttcatgaata gggtcaaccc
    54481 atttatgatt agaacttctt ttaaacaatc gttctctata atatgagaga gttgggttac
    54541 cattttcatc aaaggataca ttgtattttg ccataacaat atcaatggaa gtatccaagc
    54601 tttctttaat ctttaataat ttttctctat cagcttctaa aattatgtca tctgcatcaa
    54661 gccacatagt atagtcttta gaagctttag aaaatgaaaa atttcttgca gcagagaaat
    54721 catctatcca ttcgaaatcg tacaccatgt ctgtatattt tgagactatt tcttttgtgg
    54781 aatctgtaga accggtatct acaattataa tttcgtctac tatatccttt acacattcta
    54841 agcatctgcc tataacttct tcctcattct taacaatcat gcacaaactt attgcaatca
    54901 taatgtacaa ctcctttgtc ttctatatat tgcagacata agtaatatat ggtattttct
    54961 cttgttttgt tattaaattt ataaaaactt ttgacgacag aaagttacta aagaacctta
    55021 tcttttatgt attttttata gtagaattta ttaaacttga aatttggtaa taattgttga
    55081 aatgataaaa ccatatttaa tttcatacaa aaaagagtgt cccataaact aaaaagttca
    55141 ttttgagaca ctcttattta atattatata tatatttgta aaacagacat tttaatatga
    55201 tactttgata ctttattcat tcctattcat tcctattcat tatataatca atcagcccgt
    55261 actctttagc ctctagtgca gtcataaaat tatctctatc tgtatctctt ctaatatctt
    55321 ctattgattt acctgtattt tcagaaagaa ttttatctaa tcgttctttt attttcaata
    55381 tattatctac agctattttt acatctgttg cttgaccttt agctcctccc attggctgat
    55441 gaatcataat ctctgaattg ggtaaagcac atctttttcc atgtgttcct cctgcaagta
    55501 aaaaagcact catacttgca gccataccaa tacagatagt cgatacatca catctgacat
    55561 aattcatagt atcatatata gcaaatcctg atgtagcaga gccaccaggg ctatttatat
    55621 atataattat atctgaatct gggtcttctg attctaaaaa cagtaattga gctactacta
    55681 aacttgcaat agcatcatta atttcttctc ctataaatat aattctatct tttaataatt
    55741 ttgaatatat atcataagac ctttcccctt gacctgtttg ttcaattaca tgtggcacta
    55801 aactcatgct actttcacct cacaattaat attaaagtta agataagaat tgatttcatc
    55861 tcttctttgt ttataattca tatttgcata agaaatacat ttttcgcttt gaatttgatg
    55921 atttagattt aaaacaaatc tctgttgaat aggataaaat tcatctttat ttctatatac
    55981 ttgtggagat tctttatata aatttctaaa tgctagtgta aaagattgat gtgtagaata
    56041 tcctactgta atagctatat ctataatata tttgtctgta aatataagtt gtctagctgc
    56101 ttcagtaagt ctcctcctct gtatgtattg atggatagta catcctacaa catttgtaaa
    56161 cattctgtgt aaatggtatt ttgaataatt agcaatataa gctattttat ctaaatttag
    56221 attttctgta agattttctt ctatataatc aattatctcc ataataacat ctgtatttgt
    56281 tttcaactct atcctctcct tttaagtatt ataacctatg caaaaagttt tttcttaata
    56341 taaattgcta tcttaattta gtgtatagca ataaagtatc gataaaacaa tatcaagtat
    56401 ttttatacaa aaatattaat atatatttct tttaaagttt aaacttaatt acatctatta
    56461 tatgtagatt gctacatgtt ttatctatgt accttgattc atatgtatta ttttgctgag
    56521 tatttttaca cctttttcaa tttcatcaaa actaagatga gcataaccta gtataatttc
    56581 atggtcatat tctaatatat tctcaattgc atattgttgt ataggataaa cttttatacc
    56641 tgcttcaaaa attttatgaa ttaaactttc cgtaaattta acattataga aatgagcaac
    56701 tatatgaagc cctgctgctt caccttttat tttaaattca tttttaaatt ccgtgtttaa
    56761 aacttttatg agatatttac gttttctatt gtacaacttc ttcattctaa atatgtactt
    56821 ctctaaacct ccatttccaa taaattcagc taatacatat tgcaatattg tttctgtatg
    56881 aacatcagag tacattttta aggttttata gctttctaaa atattgtctg gtaataacat
    56941 aaatccaaca cgaatagctg gagataatat tttactaaaa gaacctatgt aaattacttt
    57001 atttgggcta agttcataaa gagaactaat tggttgaccc tcatatctaa attcactatc
    57061 ataatcatct tcaattatat agtaatcatt ctctgttgca catttaataa gctctagtct
    57121 tctttgaata ggtaaaatcc ctcctaatgg atattgatga gaaggtgtag tataaacaaa
    57181 agaaatcccc tttattgatt ttattaagtt ggtattcatt cccttatcat ctactttaat
    57241 tccaacaact gaatatccaa tagatgaaat aacctttaat aagcctttat gaatagggtc
    57301 ttctacagca acctttttat tacttttgta taaaagctgt gaaattaaag ataaaccttg
    57361 tgttgaacca gaaacaatca taatgttttt ggaattacat ctaattcctc tagtacgaaa
    57421 taaatactta gatatttcca ctcttaaaac ccatacacca gctgtacttc catatctgaa
    57481 atccgaatct gataaatcat acagtatttg tttatatagc tttgaaagtt ctttgcgtgg
    57541 aaatagtttt aattctggaa ctcctgaacg aaaatctata taattttttt gtttattagt
    57601 atgtaaatct ttttcatctc taaaagtatc atttaactta aaatcaactt tatgccctga
    57661 tattccttta gctacaattg ttccatatcc attccttgtt tccaaatatc cttctgcaat
    57721 taattgatta tatgcatcta aaactgtatt acgagataca gatagttctt ttgataaagt
    57781 cctagatgat ggcagttttt gctcaaactt taagcttcca tccagaatca tatcttttat
    57841 ctcacaataa atttgtcttg ataatgatat attactattt ctattaatcg taatccacat
    57901 tctaatacct ccaaaagtgg ttctataaaa ttactataaa aatggaacta caaaatccac
    57961 ttttaacttg ctaagattat atcataaaat ataaaataaa aaggagtcta tattatgtta
    58021 gaaatatcac ataaagttaa agaaatttat cccaatatta aatttggagt tatggttata
    58081 aatattacac attcaaaacc taataaagaa aattttttag ttcttaaaaa ctctacaatt
    58141 aaaaatatta tagaacaaca cccagaatac aatcgtaaag aaaaaattaa aacagagcct
    58201 gtttcaagtt atataaagta ttataaaaaa ttcaagaaaa cttaccctgt attgcttcaa
    58261 ttagaatcta ttctattaaa atcaaaaggt atcccagatg taggtgtaac aattgagtca
    58321 atgtttttag cagaattgaa aaatcttctt ctaacagcag gtcatgacct tgataaaatg
    58381 gaattacctt tgaaaataga attagcaaat ggaagtgaac atttttatgg tataggtggt
    58441 aaagagcata ttttaacaaa agatgattta tttttatcag ataatattgg tatattatct
    58501 agtattctta atggccctga taatcgaaca gctataacaa aagatactaa aaatattatg
    58561 tattttgtat atggacctga taaaatatca gaaaagcaaa ttcaagacca tttaaatgac
    58621 ataaaacact atatttcaag tgcttttcca gatttaaaag ttaattcaat tgatatattt
    58681 tagttaatat attaaaatcc tcataaatat tttgaataaa taaattagtg aagtatacat
    58741 tctagttaaa ctagatatac ttcactaaaa ttaattatat gtagatatta tttattttga
    58801 attttgttta taataaacac tttattacat ttatcctatg aataacttaa ttttataatt
    58861 aaagagtttc ataagaaaca atttcactta aaggaacacg agttttttca tgtctctctg
    58921 ggctctctgg tatcttagat tcatatccca tcaataatat atttattggt tctagattat
    58981 ctggcaaact aaactcttct ctaattatat ctggattaaa atagcacacc caaacacttg
    59041 ccaatccaag ttcagttgcc tgtaacatca tatggtctgt aactatactt gtgtcaatat
    59101 ctgtaagttg tctcccatca aaaggtcttg tccaaacttt gtctttatct ccacatacaa
    59161 gaattgctaa tggagcatca tatatattgg cagccttaga aagtttattt ataccttctt
    59221 tttcctgaat aacaatcaat ctttgaggtt gacgattacc accagttgga gctattcttg
    59281 caacatctaa aaccttctca agtttttctt tttcaacctt tctatcttga taattacggc
    59341 aagaatatct tttttttgta agttcaacga aattcattta tttaccccct attgttttat
    59401 taggctaagt tgatgattta tttaacctat gctataattt tatcactatg gtactatatt
    59461 acaataatgc acttttttgt aatatagtat ctaaaaagat actttaaaat aaataaagga
    59521 gctcaaaaat gaaaaaatgt ttagataatt acagttgccc aatagaagca acacttgcat
    59581 tgattggtgg taaatataag acacttatac tttggcatct taaagacact atattgaggt
    59641 ttaatgaatt aaaaaaatta attcctaaag caacgcctaa gatgttaact caacaattac
    59701 gtgaattaga atcagatggt ttaattataa gagtggttta tcctgttgtt cctccaaaag
    59761 tagaatactc gctatctgat tttggaaaga gtattattcc aattttagat tccatgtgtg
    59821 actggggttc tgattactta gaaagtctat agagcattac agtttaaatc ccattgctaa
    59881 tttaacttca tccatttgtt ctctagatgt caacatttct aaaagcttaa aagctacatg
    59941 tggagctgtt tctggacaat atgaggttat aatattttta tctacaacaa ttggttcatt
    60001 aactacattt acatcaaact cacttaactg tctttgtctt ttaccatttt tcaaatgata
    60061 tgtagtagct ttacgatttt ttaaaacacc actttttcca actggcaatg ctgccacaca
    60121 aatagaagcg attatttttt cttttgaatt aaactccctt attagattta aaaaagatga
    60181 atcatatgct tcatcataaa atccaaattc ttcaaatcct ccaggaatag ctagggcatc
    60241 ataatcatca acacatactt cttctatcgt cttatcaact aatacttgta tattaaatgt
    60301 actcataact tgttttttaa atccacaagt cactacatca atatcatgcc cataatcgtt
    60361 acgagcccat cccataacat ctacaaatac actaaattcc atagtttcaa aaccttttgc
    60421 taaaaaaact aaaactttca tattctattt tcctcctaaa atataattaa cactttacta
    60481 gtaaattatt taaaaaatat cttttattgt attaaattct tctttaattg tagataaaac
    60541 aatggttgta ttagcttttt gaatcccccc aatttccttc aaagtcactg atataaactc
    60601 ttcaagatta cttgtatctt ctgctaatac ttttattaag taatcatact ctcctgaaat
    60661 atgatgacat tctaatacag tatcatattc caatatagtt ttcttaaatt ctttcatact
    60721 tttagataaa tcagtattga taaatataaa agccaaaagc ttatattttg ttttttcccg
    60781 attaatttga atagtgtact tttgaattaa atttgactgc tcaattttcc ttatacgctc
    60841 tgagactgct ggtactgata aattaatttg tctgcttatt tcagaggttg aagccctact
    60901 attctttttt agagaattaa ttatttttaa atctatttca tccagagcta ctcacctcct
    60961 taattattta atacaatttt agctaataat ttacattaag taaataaatt taatcatttc
    61021 aataaaattt aatacaaata tgttattttt ccttaaaatt ttagttttct ttaatcaaaa
    61081 cacatataat acttaaaatt atttaagttg aattaatgtt tttattttgt ataatatatt
    61141 agaaccttaa tatatcattg aaaattcaac aatactagac atatacttct acatgttgta
    61201 atatttatga aaatagtatc cctatatcaa aagtataaaa ctcataatct taattttccc
    61261 atagactata aaattttatt ggtagcgagt cttcaaataa ctttgtaatg atagatttac
    61321 taaattaata taatatatag caaatataac tttcttattg actataacca agtattatga
    61381 taacataact taagctataa ttaataaaac atattttaaa taaataagat aatccacaaa
    61441 gtacaaaagt acaatttaaa tatagataaa ctagaattaa tactaaaatt aggatgtgaa
    61501 taattatgat ttatatagaa ttagttaaac ttgttagaaa acttaactct acacaaatgt
    61561 atacgatgaa gcgttgtgta gaggttaatc tgaaagctta aagctttcta tcttcatcgg
    61621 caaatatagg tttagtatag ttttttggct atgcttattt aaaattacct atttgccgat
    61681 agatagtata gattttctgg tcatagacaa caatgcttgt ctatgacctt ttgtttttcc
    61741 aaaagcagag gtcggcattt tacaatgcca ttcctctgct ttttttattt ccataaattt
    61801 tttaataaaa gaaaaggagt ttgaattatg agtttattaa aagtaactaa tttatcacaa
    61861 tgttttatgg ataaatcact gtatgagaaa gctaactttg atttattcaa aggtgaacat
    61921 ataggtgtag ttggccaaaa tggtacaggt aaaagtacat taattaaaat tttattggga
    61981 gaagttgttc cagattcagg agaaatcaag tggcaaccta atatcaatat aggccattta
    62041 gaccaatatg cagaaatcaa tagagataca acaatatcac tctatttaca cactgcattt
    62101 gaagaacttt ataaaataga aaaggaaatg aatttactct atcaaaaaag tgctatatct
    62161 gaaaatgagc aatatctaat caaagcatca gattatcagg aacaactaat agcaagtaat
    62221 ttctattcta tagataatga aataaataaa atagcaaatg gtcttggatt agattcaata
    62281 ggaatgaatc gtgttgttga agagcttagt ggtgggcaaa gggcaaaggt aattttagca
    62341 aaattgctac tttctaacca tgatgttcta ttattagatg aaccaacaaa ctttttagat
    62401 aaagaacatg ttgaatggct ctcaaattat ttaaatacat ttaatggggc atttattgta
    62461 gtttcccacg attttgattt tttagaaaaa atttctacag gtattcttga tatagaattt
    62521 ggtatgatta aaaaatatca tggaaaatat tcagagttcc ttaaacaaaa atcacattta
    62581 agagaagatt atattagaag gtatcaagca caacaaaaga aaatagaaaa agaagagaca
    62641 tttatacgta aaaataaagc tggagtaaat tcaaaaattg caagaggaag acaaaaacaa
    62701 ttggataaaa tagaaagaat agctcctcct agttttacag gaaaaccaaa tattcaattt
    62761 tcagaaattg aaatatcggc tcaaaatgct cttactataa caaatcttga agttggatac
    62821 tactattctt tacttccaaa attaaatttt tctgttgatg gaggtcaaaa aatagttata
    62881 actggtttta atggaatcgg taaatctacc ttgttaaaga cattagtcaa agatattcct
    62941 cgtatatctg gagatttcca gttttcagaa caagttaaaa ttggttatta tgagcaagat
    63001 ttaaaatggg aaaatcctga taaaacccca cttcaaatag tagcagataa atatccaaaa
    63061 ttaaatacaa aagagattag acgtcatcta gccagatgtg gtgtgaagga agagcatgtt
    63121 tcaaggagtg tatctacttt aagtggtggt gaacaatcta aggttaaatt atgttgtatg
    63181 atgttatctc cttgtaattt tcttattctg gatgaaccaa ccaatcactt tgatgcagag
    63241 acaaaggatg ctttacaaaa tgctttgaaa cagtttagag gaagtataat cctagtttcc
    63301 catgaagaaa aattttataa aggttggatt gataaagtgt ttaatataga aaaacaatta
    63361 gtataagttt atacacaata tacaatatta tttaattgat atttcaatgc aaattttaat
    63421 gttctagtta atgtttcact gcaaataaac taaaaaaatt ttattagaac ataaagtaca
    63481 ttaccaggat ataaacttaa tgaggctatc tcaaaataga gatagacctc ttcttcattg
    63541 ttgagataac gaaaatacat ttgacttcac acataaaaga atgcctcatt aactaaaaat
    63601 tcaacttgag acatcttcat taatattaaa atctattcta atcgattcta ttcacaagtt
    63661 tatatcctag tcctttaacc gttataagta ttttaggatt tgaggggtta tcttcaattt
    63721 tttctcttat atgtcgtata tgcaccataa ttgtattgtc acaaatacta ctatatactc
    63781 cccatacttg ttcatagagt ctttcttttc ctataatttt atttacattt tgagccatgt
    63841 agcttaaaag atgatactcc cttccagtta actcaatctc atttctatct ttatatacac
    63901 ggtttccttc tatatcaata gtaatatctc ctattttaat aactgcacta tctgatggta
    63961 caatgctttg atactgttgt ctacgtaatt gcgctttaac tcgaaaagca atttctcttg
    64021 gactgaatgg ctttgttata tagtcatctc ccccactact aagacctagt attttgtcaa
    64081 tatcatcatt ttttgatgat aaaaaaagaa tagaacagta tgaaaactct cgaatttttt
    64141 tacacacttc tataccatct atatctggta acattatatc aagaatgaca acatctggac
    64201 atgctatctt acacagcgaa atagcatcta aaccattata agctttaata atgtttttaa
    64261 aaccatcatt tatgagtact tcttcaatta aatctactat atctttttca tcatcaacaa
    64321 gtaatatttt tttattaaaa tatgtatcca tattgaaatc tctccataaa aatttattat
    64381 attctaatct tgcagtatca atatcttgtt tttttatatt cactactctt gcttttacat
    64441 tactaatagt aacatctcta atagacaaat tcaatcacta tgaaaattaa gataagtttc
    64501 tttaaaataa agtgtatgat acattaaaac ctatagaaaa ttaatttcat ccaaagttta
    64561 taatattaga ctaatacact tacagataca gatacagatt aatacaatag taaaataaaa
    64621 ggagttgtct taaaattgac tttttctaat caaactttaa gttcaactcc cttttaaact
    64681 tttatatgtt atttttatta attctttata tcaattataa ctttaatttt caatagtata
    64741 tatctaccac taattgaatt acttattaaa acataaaata tctacctaaa ctatttattt
    64801 aatatctaca tatatttata tctattctta tacttacata tacaaaaaat ggttaaaaat
    64861 aaagttaaaa tctcagcact tggtactgct aaccatatcc catttatatt taatatattt
    64921 ggcagtatca ataaacctat agttataaat ccaaatgtac gtagaaaaga taatattgca
    64981 gagaccttgc catttgacaa tgctgtaaac atagacgaag taaaaatatt atacccacaa
    65041 aaaataaaac taaaagaaaa tatataaaat ccagaccttg caatttcata aactcggctt
    65101 cctctttgag aaaaaatctc tacaatgatt ggtgaacatg atatagaaaa gataaatacc
    65161 aataaagatg ccactgaaat aaataatata caaatcttaa aaacatgatt taactgtgta
    65221 ttattttcac ttccatagtt ataacttatt attggtgcaa cacccattga gaaacctata
    65281 taaagcgaag ttaaaagaaa ttgagaataa ataattattg taatagctgc aactccatcc
    65341 tcaccaatta atttcatcat catagcatta aataaaaaag tagtaactgc tgttgatagt
    65401 tgacctacca tttcagatga accattaaaa caagcttcaa ataatattct tttatctagt
    65461 ctgggtatac aaaacttaag taactcttta ttttttgaaa agaaaaccat acctgaaacg
    65521 gtaggaatta ggtatcctac tccagttgca agtgctgctc cactaatacc catttgtaac
    65581 agaacaatga acacatagtc aagaattata ttagcacacc cagcacctaa cactagccat
    65641 aatccaaatt gaggctttcc agctgttaca aataaacatt gaaataatac ttgtagtata
    65701 tttgcagaag caaaaaccaa gataataaaa agatatgaac gacaatattt aattaaaact
    65761 tcacttgctc ctaaccatat aatgatataa tccataaata caatacctat accagtaatt
    65821 attaccccaa ttacaactcc tgataatacg attaaagaaa aagctttatt tgctccctca
    65881 atatcaccat ttcccatctt acgtgctaca atagcacttc ctccagtggc aagcatagtt
    65941 ccaattccaa ctataagatt aataattgga catacaatat taattgctga tagagcattt
    66001 gtattaataa atcttgatac aaatatagta tctacgattg tatacaaccc cataaaaatc
    66061 atcataaaaa tagtaggaaa tgcaaacttc aatagagtaa ataaattaaa tctttgtgcc
    66121 agtaaattat cttgctcata aagattatct tgttcatgat tttctgaata atatacatca
    66181 ctcattttgt ttatttttct gtacattaat tatttttaaa atatattcca aacaattaga
    66241 catttacaga cctcctttct gcataagaat tatatctaat tatttggata tagttttaat
    66301 taatcttgct tttttacaca attatatttc tactaaagaa acttttaaat ccttacttta
    66361 atcattttat agctttcagt gcctttagcg caaatacttg ttcacatagt ttagtgtcat
    66421 atgaaacgta tttatatata gttatacatt gtctattttt actaatttat taactacttg
    66481 cacttttata atgtctcata cctaacttcc aaaatctcca cataaacaat gtaaacaaaa
    66541 atacaattaa aattgagtat attaatccat taattgttat tgtcccagcc aaaatttgag
    66601 tcggaattgt cgacataatt ccatatggta agattaaata gaataatact ttaaaaacac
    66661 cttcatatat tacaccagga attttcatat tcattggcaa taaattatct tctaatctca
    66721 ttatcgcaga agatgatata acaaaaaatg aaactgttct aagtatgata catacatcat
    66781 aaaaaagtaa tgtcatcaat aacaccatga aaatatatat tattatcata actaaagtta
    66841 tctctgagtt taacttagat attccatata aaacaataag tatacttgct agaattaatg
    66901 gtgatgaacc aatattaata ttttcaaatg ttaacctaaa tagtgggtct aatggttttg
    66961 taatatagta gtctaattct ccttcttgga tttttcgtgg aatatcataa ataccaaaaa
    67021 agaaaatcgt catatttatc gcatttatta atgagaaagt tcctataaat attaacatct
    67081 ctccttgttg ccatccccct attgagtcta catgagaata tatactatta aacattagaa
    67141 gttgtaatat aaataatgaa ctatctacaa aaaatgctcc aaaaaaatca aatctaaata
    67201 ccatcatctt tgataatttc atttttaata acataaatat tattttcaaa ttttttttca
    67261 tcatataccc accccatcaa ataatgttct cattctttta tacataactt tatttactac
    67321 agaaaaaaat aaaacccata atgctagaat aattaatcct tgtattgcct cattgctgtt
    67381 tctacctatt agtaacatag atggcaaata tgttgtataa taaaatggaa ataccttcat
    67441 aactgttact atagtttctg gtaagagtac aagtggtatc attgttcctg taataaattc
    67501 aacaatattt tcaattatca ttctaaaaaa actaatctca agaaatttaa atgacaaaat
    67561 cccaataaag aagtggagtt tcatcataaa aataaaccct agaaaaatca tcaaaattga
    67621 aattaatatt tgaactggat ttgatgtaaa ggcaaaatct attttaaata taaaaatcca
    67681 tacaaatgca gaaatcatat taaatacaaa ataaaaacat gtcattccaa aatttttagc
    67741 aataaaataa ttttgaacat taattggaat aatcatgtac tttgaaaatg tgcccaattt
    67801 tacacgatta catatctctt cactggtact ctttgacata tcaatacctg ctaaaaatga
    67861 gcttattata tagtaagaca acatagagtt aaatgtaaaa ccagagacta ttttattttc
    67921 accaaaaatt gctttccata aaatataagc aaataaaatt tttacaatcg taaacaccat
    67981 attaaccacc acatcaaatc tccagataag ttgcacttta aagttaattt tggctacttc
    68041 ataatacctt ctaatacttt gtttaaattt ataaaacatt gaataatttg aacttgcttc
    68101 attatagttg ctcataatac tcatcacttc ctttcttata tattctctct acaatactac
    68161 ctatatcttc ttcttcaatc attacatctt taacatcata tttatcaaaa atccatttaa
    68221 tcgtttcatt tcctctttct tttagcacaa ttactgatgt cttatgattt gtttgttcaa
    68281 caactttaca atcttttgga aatacaatgt tagtttcatt ggaaaaacta actgttattt
    68341 ttttatattt ttgaaaatga ttaaatagtt tctctgtaga accatcatat attttacggc
    68401 tattttctac aacaatactt ctactacaga gactcttaat atcttccatg taatgagaag
    68461 taagtataat agtagttcct tttgtttcat ttacttcttt taggaaatac ctaatttgtt
    68521 tttgggctat agcatctaag cctatagttg gttcatccaa aaataatatt tttggattat
    68581 gtagaagggc tactattaat tccattttca ttctttctcc taaagaaagc gtcctaactt
    68641 gaacatttaa atagtcagat acctcaaata attcgataaa gtaatccaag ttttttttat
    68701 attcttcatt agatatatca tataattctt taaataaaag aaacgtatct gctggtgtca
    68761 attcaaagaa tagttgactt ttttggccca taacaacagc atattgtttt ttaaaattat
    68821 tttctaattt gttagggtaa taacccataa cagatatttc tccagaagac ggtgcaataa
    68881 taccagttaa catcttaata agagtagttt tgccagctcc attttgacct attaatccaa
    68941 caaattctcc ttcttctatc tttaaatcaa atgacgatat agctgttttt tctgtatatt
    69001 tcctattaaa taaagacttt acactcccca taaatcctgc ctttttctca aatcttctat
    69061 aagatttagt cagttcatta agttcaatta tactcatttt aactacctcc atatttcata
    69121 atttatataa aaaaagtcca caggtatata tctgcctttt tctgtgctta tatttaacag
    69181 cacaaaaaca gaaaactcct gtggactaga aaaattaatg ctctctattt caaaatagtt
    69241 atcaaaataa tactatacaa aattaataaa agtgtagcag attaaagcct actacaccat
    69301 aatttaacaa agtaatatat attttaaata ctatttgttg tcatatgtca gttatctata
    69361 aaggcagaat aagacacaaa aattaaatgt attaaaaata cacttaaaat ttctgattat
    69421 acagaatata tgtgtctcca actatttaat tgttggttac cattatataa ctccgacatc
    69481 aaggcactcc tctcataata ttattattga tatcatatca taaaggttat ttatgttcaa
    69541 gtatggattt atatactaat acatttagct tatttcttca gggttgaatt taaatttcat
    69601 ttataatatt cgtttataaa ttaaaaatat aatacgtata ttaaaactgg ttattttact
    69661 gtaatatttt tattgtaaaa gatatacttt tacctataca ggatgtaatt tctttagttc
    69721 tatattataa taatattgta ataagagttc aacttataat acagtaaaat ataaactgta
    69781 aatcttaagg gggtaatcta tggaaaataa tacaatacaa aaacaattaa gagatttatc
    69841 aaaaaaaatt tggcatattt atattttaaa tccagaagaa gaaaacctta aatttattat
    69901 ttccttatta gataacaacc ttgcattaat tggaacagga aaacatgaat tttacgaaac
    69961 attagaagaa tttattatag gtcttgaaaa agaccaaaca gaatcagagc aaatagactt
    70021 tgagattata gatgagtggt attcatgtac caaagttaca gacgaagctt ttatagtata
    70081 tggtactctt tgggtaatag aaaaagatta cctagaaaaa gatatctatg tagaaatgga
    70141 tactcgattt actatggtat acagagttac agatgatatg attaaactta tccatgttca
    70201 ccattctatt ccaaactttg accaaatgaa agatgagtat taccctaaaa caataactga
    70261 aaaagcacaa gaagcactag caatggcaaa tgaatttaaa ctaaaatcag aaaaagacct
    70321 tatgactgga ctatacaata gacatttttt tgaagtacac ataaaagaaa tgcttaaaac
    70381 tgtttcatat ggatactttt acatctttga tttagataat tttaaacagg taaatgatat
    70441 ctttggtcat gttcgaggag atgaaatact aatacagttt tcaaatattt tgactaaagt
    70501 attcgtaaat gatgctatta ttggaagact tggtggagat gaatttgctg tttttgaata
    70561 tagtaatcaa tcaaaagaga atgttgaaaa taaagcaaag aaagttttag aattatgtct
    70621 agagagtaat tcttgttcta taggtatatc taaattagaa aataaatctg atactttcca
    70681 gtctttatac aaacaatcgg attatgcttt gtattattca aaacgaaata ataaaggaaa
    70741 ctttagttgg ttttagtaca aaagatatta aaataggcaa atttattaga gtatcttgtc
    70801 ctatatacta ataagtaaag taaacaacaa gctatctttg catatattta tataaaccaa
    70861 ctattcttaa taaaactaca atagtcaaca tgaccactat tatagaatat aataaactaa
    70921 aaacattatt aacatgatta atattatatt ttaataattt tatttttaaa agtaagtcca
    70981 aaatgttata ttttttgatt aaaactgatg ttgatataaa aaattttact taaaaaaaca
    71041 cttagaaagg ctttataaag agcctttttt gcttttattg attaaatttt catataaaat
    71101 tccttattaa atttcaaatc aataaaaaaa ggctctttat aaagcctccc ctatttttat
    71161 tgattaaatc ctaatataga caatgcctta gcatcatagt aagtagtcat ctctcttgtt
    71221 gcatccttta atttacggta atcaaagtct aatgctccac caaaagctga tatttcagac
    71281 ccatcagacc tataccattt cccatcaatt tgtatatagt tccaagcatg atttaatgat
    71341 ggatttaata tagatagtga atccattccc attaaccttg ttaataaatg aaagctactt
    71401 gcaaaacttc cacatacacc acgtttatca accagattac catagactat gttacaatat
    71461 ttagagtcta catattttgc ttcggttacc aattttgcag ctctattagc tctttccatc
    71521 tcagatgcat ttctccagtc aaaactattt aaaaaattcc tgattactgt aagtgtatct
    71581 tgagcttgtt tgtaagaaat ccaatctcca tcggctatta cttctatagg gtcaagtttt
    71641 ccaaagccta ttctctgata tgagctacct gacaatgctg ctgcataaaa tcctagccct
    71701 tccataccct tatttatata ataatattta ccatctgctc gaagctcaac tccatttgga
    71761 tttcctccat agagtaaatt aaacttattt ttttaaaaca tattgtttaa atcagtacat
    71821 ttatatgttt tttttgttca tctgttttat caaatatatt agtctttgaa ttttgtttgt
    71881 attcttcagc atctttatct aacttttcta ttctcgactc aagtttcatt atttcttctt
    71941 gtgttaaatt tataaaattt ttaagcatat tttcaagctc ttcacgagtt ttacctacta
    72001 aagataagtc ctcagatact ttctcttttt tattagaatc ctctttataa tccttttttt
    72061 gttctaactc ttctaatttt tcatcattta atatttcttc ttcaattttt tcattagact
    72121 ctttagtgat taaagattct ttttcatcat ttgtatttag ataagttgaa ttttcatcta
    72181 acattgtact tttattttta tcttctataa tgctattttt aatacctatt tctatattag
    72241 aaaagttatt agttgtttct cttatttctg atttatataa gttcttaatt gcttcattta
    72301 tttttttagt ataaacctct tgtctatatt ttaaatctct aattctatca ttaagttctt
    72361 gcttttgttt cataatatta gataaataag tgtcttttcc tacagtttca tttttcttaa
    72421 ccatactttc atttattctt gtattattta tattactttc atgtctaaat gaatattgac
    72481 ttctatattg actagatatt ttcatttatt tcacctctct aaaataaatt tatgtttata
    72541 aaaaccaact taacttaatt atattttatc atcaaaagta tacatttaca aatattagtt
    72601 aatagtttta taggttcgag cattgttttc aacaaaaaat ttttattata aattaaaaca
    72661 ataattttta taaatatctt atttgtattt tataattcat atatttttac tttataaatt
    72721 agcaatattg cagttttgct cttaagaaac atctctatca tacataagca tatactctat
    72781 tatgtattag tataaatatg ccttttctta tcttacataa gacctcttta taaaggagac
    72841 tatcgtaaca aatatgtata cattatcaca tatatatatc atcatataaa aaatcaaact
    72901 ttattaaaaa aattaataaa ttgctttaaa taataccaca aaccaaattt gccatcaaaa
    72961 tcacattaaa gtaacaaatt aatggcaaat ttaatacatt aatagtatat agactcatag
    73021 aatgaacaaa aaaactacac ttatcttcta atcgtcttat ttatttttga gatattttta
    73081 aatttctttt ctttttttgc taagtattcg cttttattgt ctgcaaatac tgcttcagtt
    73141 ttaagcctac gataggattc ccatctttta ggcgaaagtt cttgatgagc aatagcttcc
    73201 cagatagcac agcctggttc tgaggtgtga gtacaattac gaaaatgaca ttttccgaaa
    73261 taagcttcta tatctgcaaa acttttatct aaaccttctg aaacatccca cattcccaac
    73321 tctctcatac ctggtgtgtc aataacaata ccaccacctg aaattaaaaa tagctctcgt
    73381 ctagtcgtgg catgtcttcc tttatcatca tctatgcgaa tttcctttgt atttaaaaat
    73441 tctcctccta gcagacaatt aattaatgaa gattttccaa ctcctgaaga gccaagtaat
    73501 gcaacagtac agcctgattt gatatactta gaaatctgtt tataaccatc tggctctata
    73561 gatgaggtaa ctactacatc tacacccaag gctatagaat taactacttg taatttacga
    73621 tgcacattgt cacataagtc tgatttagta agtactacta ctgggatagc tccactttcc
    73681 catgttaaag acagatacct ctccaatcta tgaatgttaa aatccttatt cagtgacatg
    73741 cacaaaaaaa caatgtcaat attagctgca accacctgtt cctcatttac ctttccagca
    73801 gccttacgca caaacaagct ttttctatga agcacatgat gaataatagc attaccattt
    73861 ttattattgc aacgatccaa cataacgaaa tcaccaactg tgggaaaatc agattgtata
    73921 gtagcctcaa aacgcagttt tcctgaaacc tctgcaaaca gtacaccatt tacacataca
    73981 gcttgataaa agttcttttc ctgcgagata attcgagcaa ggtataaatc gtgatattcc
    74041 atactttgag taataaaatt cctatttaga ccatagtctt gtaaattgat ttgtttcata
    74101 ccattcattc tgtttcctcc ttttccaact tttcaatgat tctttctagt gggaaagggg
    74161 gctgtgccag tggttttaat gcaattgaca ttaatttcac tgaattatca tcagctgcaa
    74221 tacgattaga actaagatgt ccacagcgtg tacaacgatg aataattgcc cactcaccat
    74281 tttttctcac ccacactcca ataggttcca tattagcatc acaatctgca gcacggtcac
    74341 caggaatgat atctacatga agactatgta aacaatgtgg acaatgattt ctatgttgtg
    74401 taccagctcc ttcaggaact acaaggcaac cacaattttt gcaaataaag ttatccatac
    74461 aggaattttc ctgataataa gctcgttttc tatttttata attcattttt atttcctccg
    74521 tatcttgtat tttctacaaa aaaagtccac aggtatttat atctgccttt ttatgtggta
    74581 tacatattta cctacacaaa aacagaaaac ccctgtggac tagaaaattt agtatcttct
    74641 atttcaacat aattatattc aaaataatac tgtgtaaaat taataaaagt gtagcagatt
    74701 aaagcccact acaccacaat ttaacaaagt aatatgtaat attattattt gttgtctaat
    74761 gttagttatc tataaaggca gagtaagaca caacaatcaa atgtatcaaa aatacactta
    74821 aaaattctga ttaaacagaa cccatatgcg tctccaacta tttatttgtt ggttaccatt
    74881 atatataact ctgacatcag gcaagacact ccttcactaa taattattat taaaatttta
    74941 tcataaaata taagtatgtt caagtataat ttaatatatt catatattta tgtttgtctc
    75001 attttcttat tctataaaat ctagtggtat tttaaaattt aatgttcacc tagtattaat
    75061 aataaaaatt aattgctatt atttgttttc tcattttatg cctattgctc aatttatctc
    75121 acttaaatta ataacaactg tcttttcata caaaacctac aatcaagttt ttccatatat
    75181 tctacatgaa tagtctattt tttttatata aaataacgtt catatttgta aatttattta
    75241 attttcaaat aagtatattg aataataagc aattctattg tattattgta attgtagaat
    75301 aaaactaaat cggcaaaact agagaaattt agtgacgcaa agctataggg actaacgctt
    75361 ataaaataaa ctatgagtta tgtcagccag ttgccaaaaa gagatattct ttttgttttt
    75421 atgaaagttt tttaggggga taataatata tatgtttaga gataaaatgg atagatgtac
    75481 acatatgttg actgcttata ttggtagttc atatgattat tgtgatttta tagatacaca
    75541 actagatgat tttatactag agtacggaga aaatgtggta gaatcttgtt tgcatcaagt
    75601 gatggtatta gtaagtaagt ataattaaat agatttggtt ttctagagaa ccctttttag
    75661 ttttatctac tttaaatagc cttatgttag aaagctaatt aagatagatt tctagtattc
    75721 ataattttat tttctattta aaatacatat aataaagatg taaatttgta tcaaataata
    75781 aatatgagaa cttaaattct cttatctatg tgaaaataaa catctcttaa aatgaaattc
    75841 tgtaaacata aatgattttt tagtttgaag gattagatta acaaaattat aaatttgcaa
    75901 ataagctata ttaaaaaata taataatttt ttatataact tattctttta taacttttaa
    75961 ttttaaaaga ggagaatgta tataagagta ttcaatgtac tagaaaaaat caaaagaaat
    76021 attagaagaa atggatattg agtaatcaaa taaaagattt tgatattaca aatgatatgt
    76081 ttcaagaaag cattcctatc acaacaaaaa ctattcttga agacatgcct tcaaatgatg
    76141 aattaaatca tgttttttca aaaggttgtg aacgaaaaat gaaaaagaga aaaattgtat
    76201 taatcacact actattaata ggtgttttgt tattaggaag tatcctatat aatctttttt
    76261 tagtaaaagc agctaatatt tcaatgctaa aggaatcttg gaattttgat ataccaatac
    76321 ctaacaaaga aattgaagta tttgatactc aagatagtat taatggagat ggacaaagtt
    76381 attttattca aggattttct gaaaagaatt tcaaaaaagt ttttaattta aaaggtggta
    76441 tagttgtaag caaagataat attaatgaaa tagaaaaata tattgataaa tttaaaagag
    76501 attctgttaa cattaataaa agtaataaaa ataaaattga agaagatttt aaaaaatata
    76561 agttagaagt taaaaaagat gataagtata tatataagag aaattatgaa aactatgtag
    76621 ttttaattat aaaaaaagat gaacaaaaat tatacagttt aatatggaat cagtaggtac
    76681 atatgtatta ttaagaatta taaataataa aaataaattt tagtcaatct acaaaaacct
    76741 tttaaattgt ctggaaatca cctcctcatt tgttataaaa acatacaaca aatgaagagg
    76801 tgattttatg gtttttaaaa aaataattat aagtggatat gtgctttcac atgtttatca
    76861 acatttgtaa tgcctatatt tgctaatgaa ttataatata aaaatattaa ataatattca
    76921 actatagatt atgtaaatta tcctatgaaa aaaataagtt gtataaaatc tggcaactgt
    76981 agattgacga tttcttttaa aattacaatt ataataagtt ttatagaatt aaaaataaaa
    77041 ctttagagat ttttgataac attgtagact aaatttgcca ccgaaattac tttacagtgg
    77101 caaatcggtg gcaaatacaa tatattatta gtatataatc tatataaaca ttactattgc
    77161 ttgattatct tagctacagt ttcaacttgc tcatcatttt ccaaacttat attaagatct
    77221 tcatcaatga taggtagctt aaaagtaatg gattttagcc attgtccatt aggctgcttt
    77281 tcttcataaa tttgaatttc tgaaatgagt gcttctatta actgcctgcg ttcaacatcg
    77341 ttcatgactt tataaagctt gtcaaaataa atcaatactt tatatatgtt atcaccagta
    77401 agtttctcaa cctctatcgt ttcttttttt gctttagctt ctattaactg actttctaat
    77461 tcctcaattt tatcgtacat acgataaagc ctatcatcta aatcattttt tcttctctta
    77521 taatgtttat cctctacatc taaattatct atttcctcaa taagcttata ctttgtagaa
    77581 tgactttttc ttagttcatt ttggtaatta tctatctcat tttcaatagc agatgtatca
    77641 accttcatgt taattttttc ttgtatcata gatgcaaatt tagggttgct tactaacttg
    77701 attattacct cagcaacggc atcatctaac aattcttctc taatttgttt attataggta
    77761 catttatgtc ctctattcat aagtctatgc ttacaaccat agtaatagaa gtctttatac
    77821 ttcgtcccat cttttttata ttttatagat ttattcccaa acattccagc tccacatatc
    77881 ggacacttta ctattcctga taataagtgt gtcctcatgt tcttgccctt atttacatgt
    77941 tcatactttt tagcttgtgc tttcagttta acttgtgcag cctgccacaa ttcatctggg
    78001 attattgctt catgaatgcc ttctgctact aaataatcgt cctgctctac ttgtctgtat
    78061 tcattccttg taccatgaac tttttctaaa gttcttcttc caaatgctat tttcccgtta
    78121 tacacaggat tctttaatat ttttcttata agacctgcgt caaacaatgg atttttccca
    78181 ttttgtcttg gtattttcct tataccatga ttttctaaat atttagaaag accattagct
    78241 ccaatacttg tatttacata ttgatcaaaa atagttctta ttgcaattgc ttcttcctca
    78301 tttattatca atttcccatc aataagttga taaccatacg gagcaaatcc accattccat
    78361 ttaccctctt ttgccttttg aatgcgacct tccattgttt ggacacgaat gttttctctt
    78421 tctatttcag ccactgccga taaaacagaa atcatcaatt tacccgcatc tttagatgaa
    78481 tcaattccat cttcaacgca aataagattt actccaaaat cctgcattat ttgtaaagtc
    78541 gaaagtacat cagcagcatt tcttgcaaat ctcgatagtt taaatacaag aacgaaagac
    78601 actccatcct ttccggattt tatatcttcc atcatacgag taaactgaac tctaccttct
    78661 atagattttc cagacttacc tgcatcttcg tattcaccaa caatctcata atcattatag
    78721 agagcaaaag ccttcattct tgatttttgt gcctctaaag aataaccatc tatctgtatg
    78781 gatgtagata ctcttgtata gagatatact tttatttttt cttttgacat agcattaacc
    78841 tcaatatcct tattatatat ttatatattt ttaccaataa aggtttggac tttaatctca
    78901 agtatattat agcactttta tttctctttc tcaatccgtg cttttagcta tatcaaattt
    78961 attcttatcc tctgtatttt ttactggagt aggatttgga agattctcta aatctaaaac
    79021 tccagcatat tttgtaatca gatttgctat caaatcagcc aatccattcc agtcattatc
    79081 cactaataca cctcctccac tttaaattct ttataaccaa atattatttt tcttactaag
    79141 ttttatgaca ttggtaggtg cattggctgc tacataggaa tctcacctcc gccccttgtt
    79201 tcagacgagg cagcttaaac tattgaagta tcattatcac cacttacatc atcgaaataa
    79261 gctgccacgc ttatctttta tgtattccta tctatcgctc attttcttct aacctcggca
    79321 ttatgccggt attcatttga accgattttc atcagcagaa aagtcttggc gtaggtcata
    79381 actctcagta agtagataaa gtcctacctt ggtctattca gttgtcaaag atcaatattt
    79441 gaaagggact gttttcttta cattttccta tttgccataa gcaggcgtat ctcctactca
    79501 agaaagtaaa ggaggtcaaa ctccccttca ctttacataa ggaaaatttg ccccctttgg
    79561 ttaccaaagt tttaaagttc ttcgataaat tttttcagct tttcaagaat tttgtttctt
    79621 cttttgatta aagccggatg tgtaatactt ttgagctttg ctactgaacg aacagtttca
    79681 tcattgaaat acaagcgttc aataatatct ctttcttctg cattgagcct tgttatagca
    79741 cttcttactg cttcaatcat catctgtgtt tcaacaatct tttctacatc aacactttca
    79801 tcaacaatat tatctgtaaa gtgtccgtca tgatccaatg ctgaaaaaaa gagcaagtgg
    79861 tttttcctgt ccacctgctc taaatacttc tcgtgttctt tttctcgcca gtagactata
    79921 tatatatctt cactgacttt taccttttgt cctctgatat aaagataata ctcttttgcc
    79981 ataaagtttc ctccatctct tttttatctg cttgtttttc agataaaaaa ggaggacttc
    80041 tgcatcttgg cataagaagt cctctgaaat gaaagaataa aaatatttct ttatatgaac
    80101 ttgattgttt caattcgcac ctgaaacaat catctactat acatattata aaaaagagga
    80161 atgtattgaa ttctaaatat ttctaacatt aaacgtttag atgcttccat atttatttcc
    80221 tccattttat caataatcta aataaccatc atattcatct actacttata taattgagtt
    80281 ttattaaact ttttaagttt ctattctatc aaatatttaa acagtctctg catccgaaaa
    80341 gactaactca tatagtttta ctaacaatat tttcattagc gtgttcaaat cctgatttct
    80401 tgtatcaatt attttctgtt cactttcaat aagaaaaaag cctttacttc catggctaat
    80461 aataagtctc tatttttcaa taactattgc aaaattatat cttgtttaag aaaatatttt
    80521 aattttaaag ataaaaaatt cttcatcctg caatgcttta tattctatat tgtaattatt
    80581 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
    80641 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
    80701 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
    80761 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
    80821 ctcatctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
    80881 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
    80941 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
    81001 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
    81061 ttccagtatt attttaagac tagcaagagg agttttaagt tcatgggatg cacctttaaa
    81121 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
    81181 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
    81241 atcattagag ctaactttta aacgcgtttt tttatcaagt ttcatcattt tatcagtaac
    81301 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
    81361 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
    81421 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
    81481 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
    81541 attatctttg atttgtattt cattcttatt atttttttct tttataaatg cttttatttc
    81601 actactctta gaataaagct ccaaagtttg ttctatatat tttatttctt ttccattcat
    81661 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
    81721 tttaggaaag ataaaaaaaa ctaatgaatg aactaatata attattattc caagaacaga
    81781 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
    81841 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
    81901 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
    81961 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
    82021 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
    82081 ttaaccgata gatcattata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
    82141 gaatctattc tagcctttaa gaccggcaat gaaaatggct tttctacata tccgtctact
    82201 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
    82261 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
    82321 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
    82381 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
    82441 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctctaca
    82501 cttatattat acagcatatt atttgatatt taaaagccaa aaaatttttg gtgctaaaaa
    82561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
    82621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
    82681 tatgaattta ggtaatacat ttatatctaa acttgataat gtcttgttaa atccttcagc
    82741 ttctgcccca ccacctaatt ggctagatgc agattgtcta gctatttgtt tagctatatc
    82801 accagtaacc ttattcaata tattattccc aagcttatca gctgtatatt gagctaagaa
    82861 ataagaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
    82921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
    82981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaacaaa
    83041 taatttattt gaaattgagt agatacctga tatagattgt tgtaatgcag ggtaatttga
    83101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
    83161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
    83221 atcttctgta ttaccataaa ctttagcagc tgtatgaaca tcagtgatta atgtattttc
    83281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
    83341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
    83401 tatttttatc ttatctccaa ctttaagatt atttttttca gctaaatctt tatgcattaa
    83461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
    83521 tacaaatttt gtttcttttg atgagtcgtt aaccccagtt aacataactg ttcttttaaa
    83581 attcttcgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
    83641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
    83701 aaatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
    83761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
    83821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
    83881 aataactaat ataataatta atgattttaa gctttttcta gttacataag caaatgcatt
    83941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
    84001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
    84061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
    84121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
    84181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
    84241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
    84301 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
    84361 atattatttt ttctatgatt actatatcct ttcttttgta tatcttcatt cttaaacaat
    84421 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
    84481 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
    84541 gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata
    84601 tccattattt tctcctaact tatttttgat aatatttctt taggcttctt gattaatatt
    84661 aatgaagaag caaatacaac tgataaaaca ataatactta ttaatattaa ataactttgt
    84721 ccaagtgttg ttatatttaa cataaaacta ctattattta ttaaacttcc accggaaatc
    84781 attgagtcct ctgagttaat gaatccatct acaattactt ttagtaatac atttcctaaa
    84841 aataaggaag atattatact tggtatcgat atgaatatta actcgaatat aaattgcatt
    84901 ataatttgta tcttagatgt tccaatagat aaaaatatac ctatttcata aattctttct
    84961 cttaaccata gaatcaagat taatgaaaga acaaccatcc cacctaacat aatagaataa
    85021 gtcattattt ttattatatg ttttattcca ctcactgact ctaaagactc ttcaaacgca
    85081 ttagaatctt tttcaacaaa atactttgac tcatcaattt taagctcttt caatttgttt
    85141 aaggctaagt ctgtagattc tgcactacca gaatacatta aaattttatt tgcgatttta
    85201 ttattttctg atttatttaa tatctcttgg cttgttgaat aatctacaaa caccatattt
    85261 tcactaaaat cagaagataa tcctgtatat gtttcctgtt ttttaccaga aaagatccct
    85321 ataattttaa atttatgact ttttattttt ccactttttt caatatctag taattcaaga
    85381 tcaacttcat cacctaattt tagattgttt tgtttagcaa attcttcatg aacaataatt
    85441 gaattcttat cattttctcc tatatttttg ccttctttaa ttgtaaatac tccactacta
    85501 aataaaatat ttcttttagt attatttgta gcttcgagtg aaacaacatt cttaaattcg
    85561 tcagataaat cttctctatt tattctttgt tctccgctaa ctactttagc atcttttagt
    85621 tttgctaatc catcatattg aattattttt tcttcaattt cttttaattt ttcaatatct
    85681 ttaaattgat taacattaaa atatttacca tctttttttg ttattgatat tgaagaatta
    85741 gaactttcat ataaagcctt ttctatttca ttgcttgatt tcattattgt taaacaagaa
    85801 tacaagcaag aaagaactat agttaatatg ataaaaatta ttaaagttct atttctcttt
    85861 cttgtaatat atgctatagc attttttatc actttcctaa ctactcctct ctattttctt
    85921 actccataac taattgcatc cactggacaa gcatttttac atcgtccaca tctgatacac
    85981 tccagatgat tgcaattctc aactggatct atattcattt ggcaaacttt tttacatttt
    86041 ccacaattaa tacacttatc cttatccaat ctatatctaa atattgaaat agggttaaaa
    86101 attgagtaaa tagctccaag tggacatata tatttacaaa aaggtctata tataattata
    86161 gaaagtaaca aagtaactat tagaatgatt cctttccaat aatacaagaa acctatcgca
    86221 tttctcatac ttttatttaa taaaacaagt ggtaatcctc cttccagcat accaatcgga
    86281 caaataagtt tacaaaaata cggagaaccc tgacctaaaa tatcaaccaa aaataaaggt
    86341 aatattatta caaatacaag taatattatg tatttcaatt ttctcaaatg tttatcaaac
    86401 ttaaatgttt caactttttt aaaaaaggga atcttatgta gcaaatcttg aatcaatcca
    86461 aatggacaca gccatccaca aacaaatctt cccaccaatg ctcctataaa aaacaaaatc
    86521 ccaactatat aataactaat cttatatttt gaatttccaa ttactgcctg tagtgatcct
    86581 attggacaag aaccttttgc tcccgggcat gaataacaat tcatacctgg aacacaaatt
    86641 ttctttaaat tcccaccgta gattttacca gtcttaaacc cttcaaagta agaattagta
    86701 attaaaaacc aaacggattg aaataaatgc ctaaaacgat ctcttatttt atatttatct
    86761 ttttttgtac taccatttat aatattttta attctatcca attccaatac actccataca
    86821 aattcttatc gccttatcca agacaacttt agtttctcca ttactatgac catagtaaat
    86881 catcgccata ctaataaaga taattaataa agacaaggca cggcttttta aaaacttatt
    86941 aaccatatac caatcaccgc tttactttta tttatttttt attttttcca attctttttc
    87001 aattacctgc ttccattcct ttgaactctt tgccccagaa taagtatcac ctacaatatt
    87061 tccattacta tcaacaaaga aagtctctgg catagcctgt ataccattca atctaccatt
    87121 aaagtttgtc ttatctggca ttaagaaagg atatgaagcc tttgtctttt catgtattaa
    87181 ctttgacttt tcaattgctt ccttgttttc accgttatca tcaacagtat ctgtaacaac
    87241 tccaacgata tttacaccct tgcttttcat ttcattctga acctcaacca aatcaggaat
    87301 ttcctttaca caagctgtac accaagttgc aaatacattt accatagtaa gatcatactt
    87361 ctcaaaatct ttacttgtaa aatccttgcc atttatgtcc tttgttgata gtttacgtaa
    87421 atctttaacc gattctttgt taaatgcttc tgtattttct aaatcagttt tttctgatag
    87481 cacaaaacca tttttaggac gttctttttt atctataatt tgaatctcag ttctttttaa
    87541 ttcatccaga agattacttt cagctccgct atttgtgctt aaatagcaat cgtattttcc
    87601 atcacttgaa actcctattt tagtatgagt atcacatttt gtaattttag atattttttc
    87661 ttctgatgta tttttttcga atataccaat agtcccaatt ctttcaagct cattttgcca
    87721 atttttatat ccatcgccca tcttttctat aactgcattc ttttgttctt ctgtcatttt
    87781 ttcaaatgtc aaaatagcat actttagttc tttatctata ggactttgat catctaacat
    87841 cgctatcttt ttatcatcca tatacttttt aaatttgtct gaaagtttaa atttaagacc
    87901 taaatattca taaacatatt ctttttttgt ttttaaagta taatctgatg gcttaaatgt
    87961 ttcattttta tccttctcat tagttgacat acctgcctct tcagaactta tagattgtcc
    88021 ttctttaatt ttattgcttt cattactttt tttacaccca actgtactta agctcaaaca
    88081 taacgtaagt aataggcatg ctcctcttga tactattttt ttcataaatt tttcctcctt
    88141 tattcctcct ttatttttac acaatagcag tttataacta ctttcaataa tattctacac
    88201 accttttatg aaacgttgat gaaatataat tttttatttt tccaaactat ggtataataa
    88261 tataaatatt atgcttaaag ttgttcttac acgattagta tatgatcgat tagtatatga
    88321 gcaagcacag taagtgtacg gacacttacg aaaaaattga tgaagcagcc ctctgggata
    88381 tgcttctttt tttatcaatt tttaacttgt tagggtatac cctaagaccc cgaaatacat
    88441 tcaaaggagg atttacctat ggcaaataga atacgaaatg aaagactgga aattaaacta
    88501 actgaagaag aaaaggctct ttttgaggag aaacgaaagc tcgcaaggtg cagaaatatg
    88561 agccacttta tccgcaaatg cgttttggaa aaggaaatat atcaagtgga tttagaaccg
    88621 tttagagatt tacagggctt actgtccaat gctacaagca acatcaatca gattgcaaag
    88681 cgtgtaaatt ccaccggcat aatctataag gacgatataa acgatatgaa aaagcagatt
    88741 gaatatttct caaaagagtt gtggcaaata cattctctgc ttcttaacag aacttctgga
    88801 ggtgattaaa tcttatggct ataacaaaaa tacatcctat aaaatcaacc cttaatcttg
    88861 ccatttcata tattgttaat ggaaaaaaaa cagatgagca aatcttagta agcactcata
    88921 aatgccatca agaaactgct catacccagt ttttaagaac acgaaatgat gttggaacaa
    88981 acggaactgt tcttgccaga caccttattc aatccttttt acccggagaa gcaagtccta
    89041 aaatggcaca tcaaatcggt cttgaactgt gtaaaaagat attaaaggac gagtacgaat
    89101 ttgtcttatc tacccacata gataaagggc atatccacaa ccatatcatc ttcaataatg
    89161 tgaatatggt aacaggcaag tgctatcaat ccaacaagaa aagctaccac aaaatcaggt
    89221 atcaaagcga taagctatgc aaagaaaaca acctttctgt gattgatgag ttttacgaaa
    89281 gctataagag aaagtacaaa acaaatggca agtcatggta tgaaaatgag caatctaaga
    89341 gaggtacttc ttggaaaagc agacttcaat ttgacattga tcgtcttata aaacagtcta
    89401 agtattggga agaatttcta aaaaaaatgg cggagcttgg ttatgaaata aagtatggga
    89461 aacacatagc ctttaaacca aaagataaga caagatttac aagagctaaa acgattggtg
    89521 aggactatac agaagaaaga ttaaaagagc gtattacaga aaatcaatct attacaactc
    89581 cacctgtcaa aagacgaatc ggaaatatca tcaatatgaa tgccaatgcc aaagtgaaag
    89641 agagcaaagg ctatgaatat tgggcaacca aacataactt aaatacaatg gctgagtcag
    89701 ttatttttat cagagaacat ggcattaact ccattaagca gcttgataaa tacattcaaa
    89761 aaagtgctga agaaagacaa gacttacaag ataaaatgaa agaaattgac aaggatatgc
    89821 agctactttc cgatacaatg gagcaggttc atactgttaa aaaatatcga gcctactaca
    89881 aagaatataa agcaaaccct tccgataaag cattttttga ggagcataag gctgaaatta
    89941 cacactatga aatagctctt tcaaagctaa aaaaatccta ttcaaagctg ccgaattcaa
    90001 aggatatttt agataaactt aataaactgc aagaaaaaaa gaataccctt attcaagagt
    90061 attcttctac aaaatctact atggacgaac tttatcaaat acgaaagaac tatggaattt
    90121 acatgggtaa ggagatggaa cgataactct aatattatat agctttctta tttttataat
    90181 attaaattga aatgcctatc aatgatgcaa gcatttttat acctaaagct aaatcaggaa
    90241 ttaaagctac ggaagtagtg ataaaatgca tactttttaa acaagtagtc aatacttctt
    90301 tcttcggatg cttctgctta atttcatttt ttatattatc taaattctga ttaagtattg
    90361 atttggagtt atcatcctcc atacctttaa ttaagtcttc tacattgtta attgccttga
    90421 ttacttcttc agtattaaat ttattttctt ggtcagcgtt aatcgtgctt ccgtgttccg
    90481 caatattgac ttgaactcca gaactattaa cattgatatt atatagagca ttattttcat
    90541 cgtaccccat atcggtagaa atatctttca tataattttc aacctcaagt acaaacggat
    90601 agaccattct atcgccaaac gcctttgcca tatcttgata tttattactg ccagcataat
    90661 accagccaaa agtatatgta ggtagattat ttcctgttat aaaggaaaat gctctataca
    90721 gtagatacgt ccttttatta ctatcattgc caagattcaa aactttacgt ccataagaat
    90781 tacttatcgt atttaataag tcatctaagc catcaacatc atattctaaa ctatgcaaat
    90841 actcttttat caacaatgtt tgctctatat aatttatcat ttcttccaaa tttgcattct
    90901 gttctccata atatgaattc ataacatttg aagctaatat cctaaatttt ctactgattt
    90961 ttctaagttc ttttttacta atctccatct atcatctctc cacaaaacgg gcaataacta
    91021 ccttctaatt tttttatact acttattcta gcggttttgc cacaacacaa atatttcttt
    91081 tcttcataat ctccagtttt tcttcctatc ggaagttctg cttccttttt aatctctttt
    91141 ccacgtttaa actttatact tttactattc ctaaaacttt tttcaagatt ctttgaaaaa
    91201 tcatttagca tttcagcaac ttgattttca actattcgtt ctgctaattc ataaatatca
    91261 tcgtctaaat aattctcgtg tgttaaacca caattaggac accaaatatc tattgtgctt
    91321 tcagcctcaa tatcttccac cgttaacata aatttttcac tacaacttgg acattttaat
    91381 tgaacatatc cctcttcatc cgctggaaca gaaatttcaa attttatatc attagccatt
    91441 taaatttcat ctcctcacaa taaaagttat aatttcacca ctggtagact acaaggtcgt
    91501 gcatctttac gtacaataac aacactattc tcagcacgtg taacagcaac atataatcta
    91561 gccttacttt gttcttttag agatgtgtta ttgttaataa tccaatccca catcggttga
    91621 gtcggatata taagtactcg ttggaattcc aagccttttg attctccaaa attataatat
    91681 ggatattcat tattgacgga tgtcctttta ttttctctca actgcattgc attaaatttc
    91741 tgcaaatact tctctacatc tgctttatca attatcataa caccatctga ttcatcttca
    91801 gctctacgca caaaatttgt agatgttctc tctggataca gactattcgc aaatttacat
    91861 ataatatcat tgtttctata agtacaatta agtgtcgttt catcaattct tacatctaat
    91921 tttctgcact cattttttaa aaactcatct attttcccac aactatattt tttatatttt
    91981 tttgatgagt gagttgaata tgtgcattgt ctaggatccc cgaccattat cacttcaatt
    92041 cttgaagcta ttaaacactt taatatttcc aaatcatacc ccaccatatc ttggacttca
    92101 tcaacatata tacagtcata cattccactg attctttcaa aaacttttcc attacattta
    92161 tcatttaact ctacaacaca ctctgataat ttatcagtat ataccctgtt atctttggca
    92221 aaatatttgt tggatgtttt ctttacgtac gtattgttct gtccatttac aaatataatt
    92281 ccttcaattc gttccttaaa tctacttcct tgataaggtc taataccatc ttttaacaaa
    92341 aaagaaaacc atgtagaaac tgtaatatta ttaggaatta caccaacttt attcttaatt
    92401 cggctaatta tttgttgctc atttgtaata gtatatgtta ggattagtat ttttttatga
    92461 gtattaagag aatcctctat aatttttctt gtttttccag atccagcagc agctatatat
    92521 aatttcccac tcatattaga ataccctcct ttatatattg tggaaattct atagctttat
    92581 cactttcaaa aatctttaaa gcaacttctg ttttatgccc cttcatatat ttaagtaaat
    92641 catctggatc ttcatatgat ttattcaata tctcgtttaa aatagacatg gaattcgatc
    92701 tatatataca cggttccaaa gtattatagt tgtaattttc caaagaacca ttatattcat
    92761 gggtactatc atcaaaatat atatttatat tctcacaaca attatctcct aaataatcgc
    92821 tatattttct atttagtgca tcaatatttc catcattatc ggttatcact gacacacgta
    92881 attctagcaa ttttgcgatt tccaaaaatc ttaaaaaaga aattccaacc gatattatat
    92941 ctattccatc attaataggc aatttaccat tatgagagtc catatatgct ctttgaacaa
    93001 ttagttcgtc cgaatcacct tcgactaaaa ttgctttatt tgataataat aatcgtaacg
    93061 tatcataacc tgcaattctt ttaaaaaata aatttgtttc atttgataaa tcacctagcc
    93121 gtagtgtttt tcctcgattt agtaaaatta aattttcaag tcctaattta tttgatacaa
    93181 aactactatg cgtagtaact attatctgtt tttcttttac tgactcattt actttatcta
    93241 ggagaatatt taagtttgaa tatgacaaat ggcattcagg ttcttctaaa agcacaatct
    93301 gcttattttc tattttatga tcacttaatg ccaattgtgt tttaacaata cattgtgtac
    93361 ctttaccaat gttattatat ggtatttcat ttaattgggt taccaagctg ctttcccatg
    93421 aagttttcgt accgaaatct acagaaattg tcaattcgtt atttgatgtt tccgttaatt
    93481 ttctatttat attagcaata ttctcatctc tagaaaattg ttctttcata agcctatgcg
    93541 cttgcgatat attaataatt tcattatcat ctaacagatt ctttactatt ctcgatatgt
    93601 atatatctga cccactggta ggagtatagc ttgtcgtatc aatcattgca gacttaattg
    93661 gaatgctcct tgcagttata ttttctctgg caaaagacat ccaacttacc tcataatatt
    93721 caattggcaa cgatgttatc ttgttcttac aaagcatttt atactcattg ctatattttt
    93781 catcaaaact aattgataaa caaatcccag gggtgtcttt agttttttca gaatttccat
    93841 cacctaaaaa acttggcatt tctgcatcaa aatacgcttc aataattatt ttaggaggtt
    93901 ctacattcac tccttcgttg acttctctaa tatagttttc cactatattc ttattgaaaa
    93961 ggtactgagt aagttcattc ctaatatttt tcccatgata agttccagtt aaaaccagat
    94021 taatagcttc caaaattgta gatttacctg ttccattatt gccaactaat atattaatcc
    94081 cattttcaaa atcaactata aaccaatctg aaaagcattt aaaattcttt atttttaatt
    94141 tcctaattga cattttatcc cccttcttta tctaatataa aaatcatgat tttagaatat
    94201 aaatattgat aactatataa atacagttat gagtttcctg tttaatcaca atcataaatt
    94261 ttttatgcta catcccttaa tattttttca agcaataatt taacaataag ttttatgatc
    94321 ctctagctcc tgaaaataaa tctatgactg taaacttaga ctcccattct ccttacatct
    94381 tcaataatta cgcactccat aatatcttca atatcacaat ccaaagcatt acaaattcta
    94441 agcaaaacat ccgtagttac attctgtcca ttcttcattt tgtaaaatgt acttttactc
    94501 acttttgcct tttccatcaa ttcattattt gtcatatcta aatcaatgag ttttttgaac
    94561 aatcttttat aactaaaaac cctcattctt atcctccgtt caagaaaact atatctagta
    94621 tattatacca caaaatatac ttttttcata aatatattct ctttaatcga acttgcataa
    94681 ataaaaaaga gccggtgcag tcctaagacc acaccgaccg taaatttatc tctcagcttc
    94741 tttgcttgct tctttctttt ctttcggttt ttccttatct tcagcctgat attttttgat
    94801 agctccaagt atagattctt tcttttcttc ctgtcctttc atctgttcct ttgcctttaa
    94861 cagttttgaa gaaagtatcc tgatattgct atgctccttg ccgttatcat caatggaagt
    94921 tctgatctgc ccaaagagtt tgacaaaatc tccctgctta aagtcctttg gaatatcact
    94981 cttttctcca taaacggagc agttgtgata taacttattt ccctcatcat ctttagatac
    95041 tacggagaag tttgctacct taaaggtttc tccgttctta ttttcccttt caattacatc
    95101 aacttctcca acgatatttc cgacaatatt gataagatta tccttttctt cccgaacata
    95161 aagcaggtct tttatctgcc cctgtcccct taaatcctca atcatatagt caaactcctc
    95221 atgcagcaaa ttaaggctat cattgtccat ataagcgtca tatagcttat ctaaagcact
    95281 ttcatcattg ataccctttt ccatgcttat aatcgcttta acaaagtctt tatgattatc
    95341 acttaacata tcttcaactc tatctctcat tgttttgtaa tccatgtttt tatctccttt
    95401 catggtaaaa gggaggttgc cctcccttta tcttaaaaat tcctgctctt ttattttttg
    95461 tttttcttct gttttacttt cttcctgata ggctcttatc tgtcctaaga tagacggctt
    95521 atcatctgat ttttgtgtgg tttcttcttt gctatgaaga ttgtcctcca catcataagt
    95581 tgactcaaaa taatcactgt ccttaaaatc actatcatat ctatccggaa taccgtcatt
    95641 atcaaggtct tttgcaaacg gatcatagaa gttaccctca tcgtcaatat caagtcccgt
    95701 tgctactctt aaatcttcag aatcaacata aaccaaatca ctaaatgtag ataactcaat
    95761 ttcatttttc atatttctta gagcttcatt ttctccctta ttttcataat caaagctctc
    95821 tgttttaata ggcgtatcat caatgtactg tgtccaagtt ttctcctcta aattaagctc
    95881 atattgaata ccatgtcttt catccggtgt attggtatag gcgataccga tatgctttaa
    95941 gtcaggatat agagtatgaa actcatcata actgtgattt tcttcatact ctctgttaca
    96001 gaagtcaatg attgctcttt ttacatcttc cacaagagga ttatcatttt tcttttcttc
    96061 cacctctccc atatcgagca gcttatttaa ctctgcaagt cttaatacct tagttttcaa
    96121 ctcatctgcc ttttcaaaag gcttttttaa ttcctctttg gcattttcaa gctgttcttt
    96181 tgtacttatg agcttttctt caagcatatt tagtttctcc ggcattttct caagggcatt
    96241 atccagtctt gtgatattac cgtccgcact tgttccaagc tctcctaaat gctttgcaac
    96301 accgtttaag ctgaaattat gagaattagt aaagaagtta tagcttacct ctaaatccat
    96361 atttctatac tttcctataa ccttgctttc attgattttt acctttgaaa tagcttcaag
    96421 taatctttct ccggctaatt tcttatcggt aattttttct cccgcaatgg taatagaagt
    96481 aaacttttct tcaccttccg ctttaggctc tacattggat atatcttttt tgacagcttc
    96541 tatgagcttt tctgttcttg cgatttcttc aggatagttt tttgccacct tatcctctaa
    96601 tctgtaacgg ttagacttat agtttgcttc aagcatttta agttttgtaa cctcattatc
    96661 caaatccatc ttttctttaa tctttggatc acccgttgcc aacgctttaa tttcggcgta
    96721 atttaagctg ctttcgtcca catcttctgc cacacgaaca ggagtcttac tggtcataat
    96781 ctgtgaaatg aacttttgct tattctctat cgtctgccaa aggtaggcat caaaggtatt
    96841 ctctgtaaca taacgataga tatttacctt ctcattttca tttccctgcc ttacaattct
    96901 acccgcacgc tgttcaagat cagctggtct ccaaggcaca tccaaatcat gaagtgcaat
    96961 cagtttattt tgcacattcg ttccggctcc catcttctgc gttgaaccca ttaagatacg
    97021 aacttcgcct tttcttacct ttgcaaagag ttcatccttt tgcttatcag aattcgcttc
    97081 atggataaag gcgatttctt ctttcggtat tcccattgct acaagttttt ctctaatatc
    97141 atcatagata ttaaattctc catctccttt tggagtagac atatcggaga aaagtagctg
    97201 tgttgaccta ttttcttttg tcttatccca aatagcaaat acatttttta cgcatacatt
    97261 tactttacta tcagggttat caggaagtag gggattgatt aaacgctgat ctaaggcaag
    97321 tttcttaccg tcattggtaa tcttaagcat attatcaaca tccggctcta caaccctatt
    97381 tctgacatca tccgctcttt cagaaaggct ctttaggatt tccttttgtt cttcagaagg
    97441 ctcagtttta ataacttcat aattagcttc aggtgtagga aggttaagca tatccgcagt
    97501 ctgaatatct gctacttcct tgaacatact cattagttca ggcaagttat agaactttga
    97561 aaatctcgtt tttaccctat atcctgttcc ttcaggagat agttcaaagg agctttgtgt
    97621 ttctccaaaa gtggaagccc aagaatcaaa atgctctaag tgatttttct ttaaactctc
    97681 atactgaaga tacctttgca ttgtataaag ctctgtcatt gagttactta caggtgttcc
    97741 tgtggcaaaa actatgcctt ttcctcctgt catttcatcc atatatctgc atttcataaa
    97801 catatcggag gacttaaagg cttctgactg cccgatacct gcaacatttc tcattttggt
    97861 atagagataa aggttcttgt aattatgtgc ctcgtcaaca aagagcatat caactcccag
    97921 ttcctcaaag gtaattacat cgtctttctt aaaatcatca tttaatttct caagccttgt
    97981 ttccagtttt ttctttgtct tttcaagctg ttttactgta aagttctgat ttctgtcatg
    98041 cttgtattct tctacatagt ttacgatttc attaatctga tcttggatgt gcttttcctg
    98101 atattcctta ctcatcggga ttttttcaaa ctgcgtatgc ccgataacaa ccgcatcata
    98161 ttcccctgtg gcaatctttc cgataaatct ctttctgttc ttcggttcaa agtctttttt
    98221 atcagccacc ataatattag ccgacggata tagctgcata aattcacgac cgatttgtcc
    98281 tgttaagtga tttggaacaa caaacaaaga cttactgcac attccaagtc ttttactttc
    98341 cattgcggaa gctaccatct caaaggtctt tccactgcct accacatgag caagcaatgt
    98401 gtttcctcca taaagacttc ttgctatggc atttctttga tgaggtctta aatcaatttc
    98461 tgtattcatt ccctcaaaag aaagattgct tccatcatat tctctgtttc ggattgagtt
    98521 aaaacgctca ttatacagtt ttacaagacg gtttcttctt tcctgatcgt taaatatcca
    98581 gttcttaaat tcttctttta atagctcctg cttttgtcct gcaagcatgg tttctttttt
    98641 atttaagaca gaagttttag aaccgtccgg atttataatc tggtcaaata cctttgtttc
    98701 tttaagattt agggcatctt caatcagctt ataggcattt acccttgatg taccaaaggt
    98761 catttctgca aggtcatttc ctttatccct gctttttccc tctacattcc attcacttgt
    98821 aatatttgaa aatttaacct taatatccca tcttgcatat cccggagttt ttaaagtttc
    98881 aaagatgaat ttttcaatat ctttaatcgg tatccaagtc gcaccaagac gcacattgat
    98941 ttcacttgct tctaattcct ttggaagaac tttggtaagc tctgcttttt gatattccaa
    99001 acgattcatt tcatagctta tcagctcttt ttcttttcca ttctcaacaa agccaagatg
    99061 aggaagctct cgttccgttt gtcttaactt tgcaaggtag ctgtctacaa tggcaatctt
    99121 atctctaata tttccgctta ggtactcatc ctttgttaca tagccgtact tatatgaatt
    99181 actgccgttt gcacaagcga aaggcaaatc tccatcttca aggttaaagg ataatggtct
    99241 ataaaagttt tgttcttctc tgatgtttag ataaatttct cctctaagtt cttctatcaa
    99301 agttggtctg tctttacctg taaggctttc catatactca aaatccacat agcctttttc
    99361 agataccgat aagacaagag cttcaaggga agtgtccaca tggtctatga ctttggcttt
    99421 tgtaatggtt ctctttgaga aaatatcccc ctttgccttg acgttttctt cttcatcaag
    99481 aatttcaatg gaagatacaa gagggaaatt gctatcctct tttaaggctc tggtattgct
    99541 taaattattt ataaagccat gcttctttga aaagttgtca tagacttcat taagcttttc
    99601 ttgagctttc tttacctcat catcggaata gtcctctttc tgcttgtaga taacatcttt
    99661 aagggcagta ttcagttcaa gatagtcctt aatcttttcc ttattcttat ccgttatctc
    99721 tttcttgatg aaaagggagt tttctctgta atagacttca tcatcaataa cggtatagga
    99781 aaagtttttg acatcatccg ttgcaggaat tgaagtgatt tcatcatcta aaagctctat
    99841 ttcttcataa gttgatcctt tggatatttc ttcacttgct tttgtcagaa gctcttttaa
    99901 gtcggcattt tctttcggca ggcacgcaag tgtgtttcca aatcttcctg aaacttcacg
    99961 cattgtgccg agtacctgct caggatgatc tacaaaatat ttgttatagg taagaccatt
   100021 ttcatcttca gcaaggtgaa tccagtcctc atctctttct ctgatactgt cccttttctt
   100081 taggaatata atatctgagg ttacttctgt tccggctatg cccttaaagg tatcatttgg
   100141 aagtctgatt gcccctaaaa actctgctct tgctgcaaga tagcgtctta cactttcatc
   100201 ctttttatcc attgttccac ttgatgtgat aaaggcgata ataccgccgt ttctgacttt
   100261 atcaatggat ttcgcaaaga aataatcatg gataaggaag ttatttttgt tatactccct
   100321 gtcatttacc ttatattctc caaagggtat attgccgata accgcatcaa agaagttatt
   100381 ggaaaaggag gtttcttcca gtcctttaat ctgcacttca ctttcagggt atagtagttt
   100441 tccaattcga ccgcttaccg agtcaagttc tatgccataa aacttggact tattcatttc
   100501 atcagggata ttgcctataa agtttccgat gcccattgat ggttctaaaa tgtttccctg
   100561 tttaaatccc atatccgaaa gcgtcttata tattccgtca attacggttt tcggtgtata
   100621 aaagctcgtt aaagtagatt ctcttgcagc ttcgtattct gcatgtgata agttctcatt
   100681 tagaaaagcc cttgcttctt tccactgacc ttccttgctt tcatcaaaga cttcggaaag
   100741 tccgccccag cctacatacc ttgctaaaac ttcctgagct gtactgtcaa gctctctttg
   100801 tccgctctcc actctgttaa gcatggaaat tgcttcaagg ttattattta acctttcgct
   100861 tggacttaac ttttcaggga gcgtttcttc tgtaatttta aagttatgag cttccgcttt
   100921 ccttatttga gtttcctcag tagtctgctc tggctgttta tatgtcaggt tttcaaatac
   100981 cctctcaagg tcgctttcca gacgataagg aatgacatcg aagcttgtta tcatcccgcc
   101041 aaaatactcg gtgttgtcct tcaccgttac cgtatttagg ttctttctca tatcatcaaa
   101101 gcgtgttatg gtatggtttt tgtccttgta tttaacttca tcaccgacaa taaagttcgg
   101161 tctttcaaga cttattttat tcagcaagtc ctcattatct gtaaagtcta caatcggaat
   101221 ttgatggttt ccacttctta ccggatcaag ccacaaatca tatcttcctg tgatttcatt
   101281 tttagaaatt tctctgactt tatattcttc atgattaaaa tagacggtat cgcctgcttt
   101341 aactgcaaac tcttctaaaa ggctgtcctc tttttcattc agttctactt cttctctttc
   101401 tttgaggtaa tcaaataagg tagcttgaac tgtgccacgc tttgtagctt cttctgtttc
   101461 ttcaggtgct tcaagctcta ctttatcttc aaagctaagt tctccattaa agacaaactg
   101521 aagctcctgc tcatccattt gttttataag ctctatatct ttaagttctc taaaggtctt
   101581 tggaaaatcc tctaaaatga gcctctgggc atttaattct ttcagttcct caaggttaaa
   101641 atatccccat tcaggctcta ttccaagaac aagtccaaag gcatcaccgc tttccctgtc
   101701 atactccgtc atataccaag tccaatttga acgaaaagga ataatatatg ctgcatgaac
   101761 ctgcttatcc gctaaatcta catcctcctg tgcgtaaatc tctggcactc tttcaagaat
   101821 ttcatcaggc attagatttc caggatcatt tttagaataa tattgcggtt cttttacttc
   101881 ttctatctct gccgtacttt ctgtttcctt ttcctcaaaa taaagttttt tagaaagcag
   101941 ctcatcaata tgctttgcaa cacttgtcca agtaaggaac acatcattac aatcattctt
   102001 ctgtaattta aaaccctttg catcgtgcca ttcatcactt cccattgcac ctgacacaac
   102061 gtgagaatgt cctccgattc catattcatc ttttaggaaa ttggcttttt cctgcaaagt
   102121 atgattttct ttaaaaaact tggtgattcg ttcttttcct ctatcaacgc cgcttcctct
   102181 tgaaaggctt tcaaggactt catcttctgt aataaagagc tttaccttcg gaagttctgt
   102241 cagattggta ctatattcct ttcgttgcag ctcaagttct tgcaacttct gataaagact
   102301 atctaccttg tgataatgat accttaatac atttctgctt tctttgtatc cttctaaaaa
   102361 cctgctatat tctttgattg tttctttcag atattcagga ttttttaatg cttcagataa
   102421 tcttttcgtt tcttgcggaa agccaccacc tctttcaaaa gagttgaaat atccttgctc
   102481 tattccttct tcgcttaaat cgtgcgttag ataccataag gattctgaaa ttctatccct
   102541 ttcatagtcc tgtgcctcta aaagttctac atttgtagca aactcacctc tgtcaagaag
   102601 ttcatttatc ctacttgctg catcactcca acttaaaatt tgtgtattat cctctcttgc
   102661 cgatgttcca taagctaaat gaataccttt atccgaatac caggaagata cttctctttc
   102721 atcaatgtaa aatccgtttc cgcctttaaa ggtatctttg aggtattctc ccaattcttc
   102781 tatgctcttg ccttttgaaa actcagcaat aacaggaagc ctaccgccat catgatttcc
   102841 tccgttaata aggacggtat ctatatcctt ctcagttagc ggaatagtaa ggcttatctg
   102901 tccataagaa ttttcgggta aagaaaaaga agccttttca gcttctatta tccctttatc
   102961 cgtattgtct tttaaatctc cactacttcc ttgatggtca tttctttgag agctgaaatc
   103021 atcgccccat actgcggatt gttctcgtcc tctatcttcc aagctgctat caactttggc
   103081 ttctctgctc tcataaactc tactgcctgt ttctgaatat ccatcaggtg tcctgtcagc
   103141 ttcttctcct tgtatagatc caaaagcatc tcgaagtggc tctgctcctc gctctgtgaa
   103201 aggtaattca gcctcatcac tgcgtaggtc ggattcggat acttccttat aaagtccgtc
   103261 tgttcctcca agctgtttag cgtattctcc ctgatttttt ctattatctc gtccgtactc
   103321 tccatttcgg agaactcgct cgttttcatt tctttcctga tcatctcgtc gaaatacatt
   103381 ttcttctacc tcctctaatt cttctttaat tttattatat cccgcttctt ttcctattaa
   103441 aacttctttt tgaagctcaa gctctttgct tttttgaatg gtttcatcaa taatctttcc
   103501 gctaatatcc gatacacttt caccaaggct cattaaggat atgctgtcaa atctttgaaa
   103561 attttctctt aaaagctcat aatccatcgg ataatctaac ttaaatcttg aagctaccgc
   103621 atagcttacc gagtccctta caaacttggt aaatgatatt ctatcctcat ccgctactct
   103681 aagttcattc attaaggtat ctattttttc atcaccgtag agtctgctta aagaaaagat
   103741 gttttcaagt gtgctttcac tttcctcata gccctcgctt tttatcattt cctttaagac
   103801 atcttgatgg gcttctttat caaatctcca aagattgacc tcgttgacat ccctgttttt
   103861 tgaaactgtc tggcttatat caaagatata gtccaccttt ttatatgtgc cgtaatcctc
   103921 taaaattggt atccctttct gccctcgcat aaccgttcgg ttaaagcgtt ccctccagta
   103981 gtcgaacttg gcacaggctg tcgcttcagg atttttatca tatatactta attgacttct
   104041 aaaatcatac ctttgattgt ttccgacaac ttttaggagc tttagatatt ctgcttcatt
   104101 atacaaaaca tcttgtttta ttagctccaa aatgttatga aaatcattta ttcgcatttt
   104161 tacctccttc ttttttcaaa caaaaaaggc ggttagattt tactctaatc accttcgcta
   104221 tataccaaat attattactc tatttcaatt ttttaattga ttcactttca gatagtatct
   104281 tcatttgttc aatagctatt gcatttaatt tttccaatct ttcagattgg ctcatgcctt
   104341 catttataaa taccgcatta agattctcaa gattagaaag gcagacaagc tgtgatatat
   104401 ccgcataatc tctaatattg ccttttaaat ccggattttc atcacgccac tccttagctg
   104461 ttattccaaa tagagaaaca ttaagaatat ccgcttcact tgcataaata aaattgattt
   104521 tttcttttga aagtttatcc ggtacaagtt tgtttttaat ggcatctgtg tgaattctat
   104581 aattgatttt tgccaaattt ctcttaatat cccatcctaa cttcttttgt tcttcttcct
   104641 ttaggcgttc aaattccttg attaaatata atttaaagta tggcgatacc caagaagcaa
   104701 actcaaacgc tatatcttta tgagcataag ttccaccata tcttcctgct ctcgcaacga
   104761 taccgataga attagttttt tccacccatt gcttgaccga taatataaaa cggttaaatc
   104821 ctgcttcatt tttaattccc tcgaattcgg gggaattaaa attcgtgtta tacatttctt
   104881 cccaaatacc taaaaactct attgtattct tgtttctgag ccacttttca atcaaagcag
   104941 aaccgttctc aatatttcta accatatccg ttaaagaaat ataatctcta ttgtctatgg
   105001 caataatgct gatttctgaa ccttcaacat ttattttaga catataatca cctctcaatc
   105061 attaaattga ttactttgca ttatagcatt tttagagcat tttttccacg ctgaaattag
   105121 tgctgtctta cttgcgatat gtcaaaaccg cttcttttat ctcattttca atcgcatcta
   105181 aaaattcttt cttagaggat ttaccctgag caatatcaga taattccatt tcccactttg
   105241 ctgtcgtttc tgccgactta aaggtatctg ctacaatcgt tacaaggctg attcctttat
   105301 gcgttgcaat tagattcttc ttatctcttt cgacaaatct cttaaagatt agattttcaa
   105361 taattcctgc ccttgttgcc ggagtgccaa gaccttttct ttccacctct acaccttttt
   105421 ctaaggcatc atttcccgca atctccatag actttaagag cgtatcttca gtaaagtgtt
   105481 tcggaggttg ggtaaatttt tctttaacct ctttattttc aacgctcaaa acatcaccaa
   105541 cacttacatc gggcaataca gcatcttcac tcttttttga tttgtattct ttaaggtatt
   105601 tgctaaagcc ttcatcctta attaccttac ctgaacttgt aaattcaaaa ccgtcaaatg
   105661 aagctacaat ctttgttgta ttttcaataa gcggatagcc tacacttgca tgaaatttat
   105721 cggatataag gaagtaaact tttgcttcac ttaaaggtaa ttcagaaaca tcttctttta
   105781 ctgaactgat tgtcggtatg attgcatgat ggtccgtaac ctttttagag ttaaatacta
   105841 ccttgatacg ctctgtatca aagtcatttt tacccaaaat gttgttgact gtactcacaa
   105901 tcatatcctc tgttaaacat ctgctgtctg ttctggggta agtaatcagt ttcttttcat
   105961 acaggctttg agtatagtca agtgtctgct tggcactata tccaaaatat ttattgcact
   106021 ccctttgaag cgtagttagg tcaaaaggta aatcaggctt tgtaattttt tctttttgaa
   106081 taacatcagt aatttcaatc ttatcgccta ctaaacttat aagctgttca gcagcaactt
   106141 catcatcaat tctgtctgtg gagagtgtaa atccattcat agaaagctct actgtgtaat
   106201 atttttcttt ctgataattt gctatctcat catctctttt tacaatcatg gcaagggtag
   106261 gtgtctgcac tctgccaaca ctgtaatttt gctgatacag acaagagtag agcctactaa
   106321 tattcattcc gactaaccaa tccgcaatag ctcttgcctg tgccgattca aagagattat
   106381 catagtcttt tccgtctgtt aggttatcaa aaccctcttt aatggcacta tcttccattg
   106441 aagaaatcca aaggcgtttc atcttctttt tacaattcac ttgattatat acaagtctaa
   106501 aaatactttc tccctcacgc cctgcatcgc acgcattgat aaccgtatca acctccttat
   106561 cattcatcag ctttttaagg atgttaaact gcttcttggt tgcctttgct acttcatact
   106621 tgtagtcgct cggaataatc ggtaaatcag ccatattcca cttggcgtac ttttcatcat
   106681 aagcatccgg atttgccatt tgaattaggt gtccaacgca ccagctcacc ctatatccgt
   106741 ttccttcata atatccgtct ttctttttat ttgctccaat aacctttgca atggagattg
   106801 caacactcgg cttttctgca ataactaact tcatctgtgt tttcctcctg ttataaatca
   106861 aaatagggcg aaagatgtta ctctcccgcc ctaactttgc acccaaaata atatgttttt
   106921 aggtgcgttt attcttcatc ttctacttca tctgcatcat ttatttcttc ctcgctttca
   106981 gattcagaaa agaaatcatc atcttcctcc tctaaggctt caagttcttt atcttctttc
   107041 tttttcacaa ccttaaaata gtagcctgct cctaatgcac cacctactac aagaagcaaa
   107101 ataatatagg ttcctaaatt actcttttcg tccttcttct caggctttac ttcttcctta
   107161 acaggttctt cttttactac ttcctgcttt ggtgcttctt ttttctccac catgttaagc
   107221 aaatcatctt cagatacttc cgtaagaagc attacattct cactatcttc atcatgattg
   107281 atgattaaat gaaaagtctt accgtttttc gtttgaaagg taataaactg ccttgcatct
   107341 gccgaatact gatccgtttc tttgttatcg ctgctatctc catgatgaat cggatactcc
   107401 ttatttgcat tatctttatt ctccgtaacg gatgctctcg cctttgacgg agatgaagct
   107461 actcccttgt tggtgtttac actcttactt gcagacccca ttgatgaatc ctgactgtta
   107521 ttcgcaggag cttttgccgt caacttattg ggatagcgaa cttccttttc tttctccttt
   107581 acttccgttt tgcctttacc ggtatccttt gtagtattac ctgagcttac cgaatccgat
   107641 gagcctttac cggtactact tacagaagtc tgtggtgttt gaggagaaat acctgaactt
   107701 gtcttaattc cggaaatagg actaatcggt gtaactgatt gtgatgttac aggagcttta
   107761 tttgtttcag ttgccttttt ctcaagctct ttcacctttt ccgtcagctt atctatttcc
   107821 tttttcatat cttctgataa gtccttattt cccttatctt tcttcatttt ttctttcagg
   107881 ctatcaattt catcttcaag gtctttaatt ttattctttt gcttatcact taatttgtct
   107941 ttgtccttaa gttcgcaatt taacttatca agtttatcct gaagctcttt tgcctctttt
   108001 tctatttttg aaatatcgtc tttagaaagc tctgtctgcg ttcccttatc ttctgtctga
   108061 gtgcattcat cctgctttgg cttttcttct tccgtctgcg tttccttatc cttaacctcc
   108121 atctttgcta ccttacggat aatctcatct tttccatctc cctttacctc ataaaacaga
   108181 aattcatcaa tgaatttcat atcatctggg agcattggaa gatctccgct atcaataagt
   108241 tgtcctttct cagcctttat cttttcttcc ttaaagacct tttcatcttc aaagatatac
   108301 cttacattta cttcaacttc cgtttgaaca gcttcgtttt ttgttggagc ttcagatttt
   108361 tgctcctgtg cataaacaac cgtccaaaga cctaaaatac aactaatact tacaattacc
   108421 gcaagtgcta ccgtccaaaa cttattattc tttttcaaac tggttttcat ttgttttctc
   108481 ctttttcatt tctgcttttt gtctgtttac ctttgctatc aaatcgctga ttgtgatgtt
   108541 gttctttctg caaatggcaa tgatttcttc attttcaagc tcttcttcac gaatgaataa
   108601 aggctccaat tcttcatcaa tcagagcctt tttatccttt aacttcttta ttctgttttt
   108661 tactgttgta agttcctttt tcaaattgtt acctcctatt tctttacatt tggcggaaaa
   108721 ccgaatccta cgggatggtg cttacaaaat gttgctatcc aatcatctaa ggtagttact
   108781 cttattcgac cgatgttatc aatcactttt ccatctccaa tgtaaatacc tacatgacca
   108841 taggtaagcc ctgcactgct tccgctactg ctgctttcaa ctgccacaag cattcccact
   108901 tttagctttg acctgtctga tgtaaaggta tagtttcggt acatatcaca ggcattcccg
   108961 cctatatatc caagtcctgc attttgatag acctgtgata cccacatggc acaccagcct
   109021 gcacccggag atggcgtaat gtatgcagca ttaacaatct tcttttgtac ttccgtagat
   109081 gcctcatact cttttccacc gccgatacct ccatttgcac tgataaggtc agaattacca
   109141 aaggcttctc tcatattgcc ttgtgcaagg aacaaggctt catagtgctt tagattatcg
   109201 ggatagtttg caaagacctt tcggataata ctgtccatct cttttttatg aagcgttacg
   109261 atgagttttt tatactcata aggttcttcg tggctttcgg tatattcatt gccatcttca
   109321 tcggtatagg tttccgtaac cgtcctgtat ctgatttcaa tttcttccct atactcaagg
   109381 tcatacatgg actcaaataa ttcttttaat atggattcta cttccgatac actctttacc
   109441 tctccgcacc ttgatgtaat gtaggataaa agctcatgga cattatgacc gatgtattct
   109501 gtattattta agatatattc atcatatcca ggataatttt ctttcacata gtcaacttca
   109561 ctttgaagct caccctccat atctgaaaat ttctgattga tttcacttag gacatttggc
   109621 tttgataaat agcttgttgt aagcacagag cttgtagagt tcataaagcc tgtcattccc
   109681 gttcctgcaa agttaatcac aaaagtccct aagataatga tacctatgaa aataagcatt
   109741 agtccttttg cttttcggat aatcatatcc tttgagcttt tcagtgttcc gataagtccc
   109801 tctttaattc gatctctaag ccttgactta ttctcacgag aaattgctgc ctttacctga
   109861 tttttctttt gaaatctttt atatgcactt gctcttttat actcatccgt ctttttcagt
   109921 tcctttttgg catcacgaaa ttccaacttt gacttacgct ttctgatttt atagtcctta
   109981 tttgtaaggt catagcctct tttagctctt ttcttatcag agtacttctt tatgccatga
   110041 atgagcttgg agcttgtatc tgctgtcttt tctcctgctt ccactccttg attttcatca
   110101 cttccatgag acaagtaatc tcttacagtt tcacttcctt tagcaagtcc tgaaagggca
   110161 gataccttta ccatgtcttt ttttagcttt ttcttttggt cttttgatag gctgctataa
   110221 acttttccgc tgacagcatc ttttcccgcc tttttatcat cagccttatt tccctttggt
   110281 gcatcgtcct ttttccttgt ataaaggctt tcagaatagt tcttcctctt gtacctgctt
   110341 ttttgagtct tatgattttt aaaggaattt tctaatgatt tttctttatg ggaaagatta
   110401 tcctccacat cataggtcga ttcaaaatag ttgctgtctc taaagtcatt atcgtatctg
   110461 tctataatcc cgtcattatc aaggtctttt cctaaaggat catagatttt tccatcctta
   110521 acctccgtaa tataatctga tcttcccgtt tcaattgcgg aatttacgtc tcccttatct
   110581 gaagttctat atttttcgtt tctcttggat gttccataag acttttcatt atcagcactt
   110641 accttacttg ttttttcatg cactttatcc tgaaaccgtt ctttctcatg aacgatttta
   110701 cctctgtaat catcgttatg ttttagctta ctttcttcag gtatcgtatc tgttttacta
   110761 tgaaacatct ctctttcaag gcttgcctta tgcctttcct taaaatcctt tttcagcttc
   110821 tttcccatag gctacctcac ttcttccggc ttggtagtca tcttctgata taaaattgta
   110881 tctttcggaa acttatcaat gaaaggcaca atggtatttc caaagaataa gagtccctca
   110941 ccttcatttg aattagtaac atatctgagc tgtggaagtg agattttaag ttttcttgca
   111001 agtatctccc tatctcctga tgcttgatta agcattaaga caaagtctgt attgtcaaag
   111061 atattttcta tctccttact cataagtagg tctttgacat tctgcgtaat acctgttgga
   111121 atacctcccc atttacgaaa tcttttccaa atctctacgg aataggatgc tgtctgctca
   111181 tcttttaaaa gcaaatggaa ctcgtcgata tagtaccttg tagccttgtt tcctctgttt
   111241 tgtgacacct tattccacac ctgatcctga ataacaagca taccgatttt ctttaattga
   111301 cttccaagct ccttaatatc aaaacaaagg agcttcttat ttaaatccac atttgacctg
   111361 tgattaaaga cattgagaga ccccgataca tagatttcca tctctgttgc cagcttctta
   111421 ccgacctttt cttcctgccc ttttaacata tcgtataggt cttgaagtat cggcatattg
   111481 tcaggagtag gatgttcaaa atacttttca tagattttag gaaggcacct atctataacg
   111541 gacttttctt ctgctgtaag accactaccg cctactacaa gctcaagcat actcatgata
   111601 aagtttgcct tgtctttcag cggtgcatcc ccatcaccgt agttcatgtt tatatcaaga
   111661 ggattcaggt aatccttcga cttactgctg accttaatga cttctccctt aaactgcctt
   111721 acgaggtttc catactctcc ttccggatcg cagataatca catcatcatc tgttactaaa
   111781 atcgcatttg ccatttctct tttggcacta aaggacttac cgctgcccgg agttccgagg
   111841 attagaccgt ttgggttctt taatttcttt ctatctgcca taatcaggtt atggcttagg
   111901 gcatttagtc cgtagtaaag actattactt gaattgataa aaagctcctc tgtggtaaat
   111961 ggcataaata ctgccgttga tgagcttgtc aatcctctat ctatttcaat cttatttaca
   112021 ccaagaggca gcacgctcac taaaccttgt tcctgagaat ggtcaagtct tttcagctta
   112081 cagttatgct tatttgcaat ggagcttatt tgagcaattg tgttatcgag cttttgcact
   112141 gtccttgcaa agttcataaa gacgatactt accacaaaca ttcgctcatc tcttgtctgc
   112201 aagtctttta agaggctctt tacatcctca ccataggtaa ttaagtccga tggaagaatg
   112261 tccatatcat aaccgcttct taccgccttc ttattttcct caattttcat cttatcaata
   112321 tcagtgtttt ttcgttttac catcttaatg gcttccgatt ggtcgattgc cctaatatga
   112381 aaggaaatat tgatgttatc gtcaatatcc aaaaactcgg ataacatcct atctgaaagc
   112441 tcactggcaa ggatttgaaa atgacttgtt gctccgatga atttgccgaa cttaaagtat
   112501 ctactcggtg taaagttaaa ctcatcaggc acaatatatg tctttgtgct ttcccttttc
   112561 tttaggtctt tgtaggaaaa tttaaatgtt ttatttggat ttaaaacatc atgaagtatc
   112621 tttagccttt cttctccgtt taggctttct gctcttactc ccatactttt aagactcgat
   112681 aatatatcta tctccagtct ttcaagtttt gatgttgcct gttctaaatt atccgcttcc
   112741 actgtaaagg ttacatactt tgattttttc aatccgttat ttccctttac aatctggctt
   112801 ttaagcatct ctctaaattc aaagcgtata tcgtcgaaac cgtccttttt atccggtatc
   112861 tgaattgccg acttcatttc ttcgtttcgt ccgagctgat tgatatatga aaactcaatg
   112921 ctgatacttg gatcaaaaga gtttaggaag tttgcaaatt gattaaaaat caagtcccta
   112981 tcttcatcta aggcaagctg atagttaatg tcctgaaagg ctatactttt actgaagtgc
   113041 ttttcatcaa gctggcatat tccgcttttt aaaagcctta gatagggaat ggtatcttct
   113101 accgtatatc ttttcggttc tttcttaaag ataaggtcaa ggattcctcc cttatctttt
   113161 tttgacttgc aattactttt ctttaagtcc cgcttttgac ttctcaatgc cttttcgttt
   113221 tgcatgagtt ttatttgctg aatttttctt ttcttgttca aggtaaacct cctttctcac
   113281 tctcttttgc ggttgataaa acttatgaag ataaatgtac ttaaagtatt tctcaaatgt
   113341 cagtccgtct ttttcaaata gtgtgatgaa aaagatagga agcgtggata cgataaggaa
   113401 tatgacagct atgtcatttg gcacaaactt cctcataaat aaatagactg gtattccaac
   113461 cagtcctgca agtgtgaagc ctatgagctg ccttttcgtt aggttaaaag caacctttgt
   113521 ctttacccta tttaagtctt tagggattgg tacatatgcc atatcttacc tcccatcttc
   113581 tttacccttt gacagctcct caaagtgttc atatacagag ccgatttctt cctgaatagt
   113641 cgcaagctgt tcgtctgtgt tcttgttata tctttgctgt attaggtaaa aatcgttatg
   113701 gcagcgacca atgtcctctg ttttttcttc caattcttcc accttattat gaagcctgat
   113761 cctatcaatt atcgccataa tacctgctcc aattactact ataaatactg tttttctttt
   113821 gttcatatta cccttccttt cttttagtgt gcatttaata cgcttttggc cagtgttccg
   113881 ctctttaaca tcattaaccc tagcaaaacc gcatatccaa gtatcgtaaa ggtacttgtg
   113941 tgtatatctg ttatctgtat tgtcttaact aatactgcgt atattccaag acaaaccatt
   114001 aaaaagagtc cttgtaaacc tatggcaaac aatcctttga tatagtttgt tccaatctgt
   114061 ccccactctt tgtttcccat tgtggcaaat ggaatggctg aaatggatga gtaaacataa
   114121 atctcaaaca ttcttccgta aaccacaagc ataatcacaa ttgaaatgac ctgtattgcc
   114181 acctttataa gcgaggtttc aaagagtatc atgacaagct ctccaagacc tttatccttt
   114241 aatccatcta ccatttttac tatctgatct ccggagataa cggcagaagt attgattacc
   114301 cctgccgctt tatttaccat gtgctgtgcc acatcaaaga ccgccattga aaactcaaat
   114361 gcatgtgata ctaaccatac agcaatccac atcttgatga tgtacttgaa aaattcaaaa
   114421 gtatctgtat cgtgcatatt gttcttttgc attaccatat tgataagctc gatacaaagg
   114481 actgctgtga tgattagccc tgctatggga ataatgacgg aatcgttaat gcttttaata
   114541 aaggcaaata catctccgtt ccaccccata ggagtttttc ctacatctgt tgcaactgca
   114601 ccaactttat cgttgatgtc aagaaacatg gactctaagt ttgcttgaat accgcccagt
   114661 agaagctctt taaagaattc ttctatcttg tcgaatattc caaacatcta agctctcctt
   114721 ttcttattac ttgagtacat ttgcaagaag cggaatcagc ttaagtccga taaggacaat
   114781 gcctcctccc gccataagct gcttgatgcc ctgagatttc gctcccggat tatcattacc
   114841 atatccttcc ataaggttaa taactcccca tgctccaagt ccagcaccta ctgccataac
   114901 taaaatcttt aatacattta ctgcctgtgc gaaaaattcc ataatttatt cctcctcatt
   114961 ttcttctttc ttctcaatct tgttatatga cttcactaca aagtttttgt aagtctttcc
   115021 ctctttttca cgcttcttaa agtagccgaa tacatgaatc aaatcgcctt tttcaaagtc
   115081 ctctgctttc tctgcttttt ctccgtaggc tgcacaattg atgtactcct tgccctttcc
   115141 atattttttt acaagcgtaa agtttacaac ttctacttct tcaccctctt tttcaaaact
   115201 tgagaaagta ggttctgcca ctaagttggc gttgatgtta atcatttctt gtttcatctt
   115261 tttaaatttc tccttttcat ttcaataaaa aaaacgactg gagtttttct tttccaatcg
   115321 ctgatactct tactatttag ttttcgttta agtgggattt ctgatcctta tcatcttgcc
   115381 tcctgatttt gaataaaaaa acagcaaatc tttttaagac ttactgcatt cttgtaataa
   115441 ccgtctctct atttaatttt gcttttcctt ttcgcttcat atagctttct atgtcaaaca
   115501 aattcttctt gtcataatcc tcaagcagct tataattctt gtgctttgta atatcgtatt
   115561 tatctgaaag aaaaggtctg acacctctaa gctgaaatat acattttcca ccgtccatta
   115621 cagtaatctc atcttgactc atcagttcct taccagtctt ttgatagttt aaaccaaagc
   115681 tcttttgatt tgaccttgtt tccgacgtgt tatacaggtc gatagtttct tttcccagtg
   115741 tttcagacaa ttcttttagc gtagttttct ccttgccacc aagaaacaag gtactatcac
   115801 agttacctac gattgtatca gcgttatcct tgtagattgc ctttagctga gattgtgctt
   115861 gaagtatgat acttgctgaa atctctcttg aacggattgt tgcaattaac ttctcaaact
   115921 taggaattaa gccgatatta gcgaactcat caagtagaca cctcacatgg acaggtaatc
   115981 gtccgccata tacatcatcg gctttatcac ataaaagatt aaataactgt gagtacataa
   116041 tggatactac aaagttaaat gtatcatcgg tatcggagat aataacaaat agtgctgttt
   116101 ttctatctcc cagtgtatca agctcaagtt catcgtcact catcaagtcc ctaagttcct
   116161 gaatatcaaa gggagcaagt cttgcaccac aagaaataag aatcgatttg gcagtttttc
   116221 ccgcagccaa tttgtacttt ttatattgct tcactgcaaa gtgtgtaggt tctttcttct
   116281 ccagtgcttc aaagagccta tctatcggat tcatataggt ttcattatct tctctgactt
   116341 ctgaagcatc aatcatatca agcagagttg caaagttctt ttcttctctt ggagcttcat
   116401 agaagatata gccaataagt gctgtatagt agagtttctc agctttcacc caaaaatctt
   116461 cacctgattt ttctccctct cccttggtgt ttgcgataat tgtctgtact aatttgagta
   116521 tgtccttttc acttctaaga taagcaaagg gattgtattt catagacttt ttgaagttaa
   116581 tggtatttag gatttttatc tcatatccgt tatcttcaag catcttaccg cactcaagga
   116641 ctatagttcc tttaggatcg gtcacacaat atgacgagtg catctgcata aggttcggtt
   116701 ttacataaaa tctcgttttt ccacttcctg atccaccgat tacaagtaca ttcttatttc
   116761 ttgcatactt tggatttgcc ggtctgccat tcatggttaa tcgttctgtt tgcgtaagta
   116821 ggatattgtt ttgaaacttt tcatccatat atggctctat atcttttttc gttccccatc
   116881 ttgctgagcc atactctttc ccctgtctaa actttttagc atttttccct ttggtatata
   116941 cgataatttt gattaaaaca gctactacca ctcccattaa aatatccgta ggatgaatgc
   117001 ttggaagaaa gctcatggtg ttaagttcca gtataccttg aaatattttg tctattacat
   117061 cgccaccagt atatgctctt acatgatgag aaaagatatt gccaacatag aaaaatgcaa
   117121 gatagggaat gttctgcttt agaaactttg ccttatcttg caccttaaat aagcctttga
   117181 tgtcttttag tatcttatct atcataggct ttgctccttt tgtttatttt tgattttctc
   117241 ttttgaaata gagtttttag ccatctcttt gaacttctca atattcttgt gaatagattc
   117301 tttcctttct gctttctttt ctgattttga aagagcgtgc ttaaaagctt tatccatcac
   117361 tttcatatct ttggcttgaa agaatacaga gtttttacct gtctctttat ttttcatcac
   117421 tgaaaattta acaccgtatc tattcaactc ttttttaagt tctttaagct cagcttcttc
   117481 cactgggatt tcttcaagct gtcccttttt aaccatatct tttagcttta cttcatttcc
   117541 gttggcattg accagttttt caagtcctcc gaatttctcc gcttctttca tcaatttttt
   117601 taataagttt aatagaactt ttccagtaac tttaccagca tttatctcca tatttaaggt
   117661 ctttcttgct atttcttcat tgatcacatc aatatcctcc tctcatatca tagcttacaa
   117721 gagccgaata ataggcatct attccacttg gtgcattata agcggcggtc aggacaaaag
   117781 ctcttatatt tctaatccta tattcatttt ctcttaatac tcctactaag tactctaaat
   117841 gtcctgaatt aagtttccta aatctttcct ttactacttg tacagatatc tttgtttggt
   117901 tgatagtaat gaaagattca tcatctaata ccattacatc tgccagtatc tttaatattt
   117961 catcaaactc ttcagccaca ctcttattcc caatactcat atgttcataa tatcctgttt
   118021 ggtgtcgcaa ttcttctaaa caattttgat atatattttt atagcttata tctatcctcc
   118081 ctcccttcat attctctgaa aataattgat tcttttcttt gatggaagga tggatggaaa
   118141 tatatgctga attatttgta gtattatttg tagtattatt tgttgtattc tctggtaatg
   118201 gttggtgcaa attatccttt tgcattagtg cattttgaac ctgtgcatta gtgcaatctg
   118261 cattattgca ttggctgatt ttgcccttat ccattggtgc attttgccct aatgcattgg
   118321 gcatagaatt ttttaatgct tctgtttgta ttttttgttt tttcaaatcc atatccatat
   118381 ataattcttc aagtttatca ttattaattg tataccattt tgttttatct cttggatcct
   118441 tattgtaatt tccaactaaa agataacctg ctttttctaa ctttgaaaaa gttcttttta
   118501 ctgtatcaaa gctcatatag tcaaagtcat tttcttgcca tgctcgaatt gaattatatg
   118561 tccagtattt accatcatga taattctttc ctgatttttt atttatttct atccaataat
   118621 taatctgctg tagtatgagt gcttcgttta atcctatttc tctagcaaga gttttattcg
   118681 caactatagg ttgttcatca aataaataca taactcatca aacactcctt tctttttttc
   118741 aaacaaaaag acgatagtta tttctatcgt ctaaaatcat atataaatat cattttctaa
   118801 atagagcgta ttgcttcttt cactcccgtt aaaacggcta taacagctgt tccaaccatt
   118861 ggtattccgt atgggcttaa caaaaatcct aatatcaatg cttctatccc tatggtaacc
   118921 tctttttgta aaaaagatgc tattgctcca aatatgcaaa acatcatcaa caggtaaagt
   118981 attgctgttc ctatgccaag taaaaatgaa agaaatgcaa tgagaatact taacgctata
   119041 ctaattggaa ataagataat ttttaatatc catctcatat atactctcca tttatactaa
   119101 atcattttaa aatgtcttaa agcgtccatt atagcttctt ctttttcagg cgtacactgt
   119161 gggatagcct gattgtcttt tttagattta ttataatgct ctctcagttc aattccacat
   119221 ttcttcttaa tctgtgcaat ataaagtgta gaaaccttta gcccaaattt ttctaataca
   119281 tactccttaa tttgagcata tgttgctttg ctctctgcac ttgtcaaatc aagctcatcc
   119341 agcttaattt caacatctat atgcttatcg acatcaagtt tggacaataa cgctaccgtc
   119401 tccacatgtg tagaatgagg gaacatatct acaggttgtg cctcaacaca tttaaatccg
   119461 tgaacattta gatagtctaa atcccttgct aaagtagaag gattacaaga aacatagaca
   119521 attctttccg gattcattga aacaatagtc tctaatacct tttcgtcaca tccctttcta
   119581 ggtgggtcta caactactac attggcagtc ttaccctctc tatacatctt aggtacaatt
   119641 tcctctgcct ttccaacata aaattcagca ttatgtatat tgtttaattt agcatttatc
   119701 ttagcatctt ttatggcatc atctataatt tctattccat aaaccttttt agccttttgt
   119761 gccaaaaata aagatattgt acctatccca cagtaaatat caaaaacagt atcagtatca
   119821 tttaaatttg catattcaag agccttatca tacaatactt tagtttggac agggtttact
   119881 tggaaaaatg ataatggtga aatctcaaac actaagtcac ctatataatc gtttattttt
   119941 ccatctccat atataactat attttctctt cctagaatag cattcgtatc tttttgattt
   120001 acatttacaa ccaaagtctt aaatccaggt atattttcct taagcactga agctaattct
   120061 ttaagatatg gaagtttgtt tccatttgct actaatacaa ccattacttc tttagttgta
   120121 aaacctattt ttgttactaa atgcctaatc aaccccgtgt gagtcttttc atcatatata
   120181 cttaccttat aggcccttat atatgttttt attattttaa caattttatc atttatctca
   120241 tgttgtatta cgcatttttc tgtcgatatt acatcatgag tcttcttttt atagaagcca
   120301 ataattggaa taccattatt cttttgtatt ggaaattgtg ctttatttct atatctatat
   120361 ggattatcca ttcctatagt gtcatgaact tctacatcct ctaacttacc tattctcgaa
   120421 agtacttgtt taacttcatt tgttttcatt tcaagttgtt tattataatc taaatcttgt
   120481 atctgacacc ctccacaatc cctaagattg tcactacaca ctcttttcac cctgaatgga
   120541 gacttttcta ttatctcaac aatatctccc tcagcataat ttttctttac tttattaatc
   120601 ctaactttaa ctttatcatg tattaatccg ccctctacaa aaactgtaat accattgtac
   120661 tttcctactc ctactccgcc ttgaccaatg tctactatat ctacaatata catctcgttt
   120721 ttcgaaagca taaatcaccc tccaatcaat ctattatatt taaatcttta caaacttttt
   120781 tctttaaatt tatattaaaa tattgctatt aattaaacta tattttttac taagctacaa
   120841 tattttattt taccaaaaaa aaaggagctt ataaagcccc ttagtaaata aatttattat
   120901 ttttcttata aaactctagt tgaacctcta tataccacgc ctcttgatgc atccacagtt
   120961 acaacttctc catcttttac tagatttgtt atatcagttg cagacacaat aactggtata
   121021 tctaaattta tacctacaat agctgtatgt gatgtcattc caccattctc agttactaca
   121081 gctcctgctt tttctatata attgttcatt tcagcatcag tagcagtaga aacaagtatg
   121141 tcgccttcat taaagtctac acaagcattt ccattcttta ttatacgaac tttaccttct
   121201 accgtttgag tacctacacc tataccttgc acgatttctt cgcttattac atgaaccttt
   121261 attaaattag tagttccact tactcctact ggtactccag cagttataac tactaattcc
   121321 ccattatcta tataatcagc ttgtctagct gcctcaattg ccttttctat aacttcatct
   121381 gtatttcctg caacagaaga cttaactgga tatactcccc atgtaagagc taatttattc
   121441 atagtctttt cattatttgt agtagctatt attggagatt ttggtctaaa tttagatacc
   121501 attctagcag tatatcctga agaagtagat gttattatag ctgatgcatt taaatctact
   121561 gctgttgtac aagttgcatg gcttattgca tctgttacag taacattgtt agtaccattt
   121621 tcctttagta atctatcata atctaaagtc tcttctgttc tttttgctat tgtagccatc
   121681 atttttactg cctctacagg atattttcca gcagcagttt ctccagataa cattatcgca
   121741 tctgttccat catatatagc attggcaacg tctgtaactt ctgctcttgt tggtcttgga
   121801 tttcttatca tagaatcaag catttgagtg gcagtaacaa ctggctttgc taattcatta
   121861 cattttttta tcatcatttt ttgaactatt ggcatctctt cagttggtat ctcaacacct
   121921 aagtcacctc tagccaccat tataccatca gaaactttta atatttcatc taggttctct
   121981 acaccttctt ggttttctat tttagatatt atttgtatat cagttgcatt attgttctct
   122041 aatacttctc ttattgctag tacatcagat gcctttctta caaaagaagc agctatataa
   122101 tctattcctt gacttattcc aaactctata tcacttatat ctttaggagt tattgctggt
   122161 aaatttatct taacaccagg tacatttact cctttatgat ttttaactat tcctgaattt
   122221 tcaacaacac aaactatatc ttctccattt atttctttta ctcttaagcc aactaaacca
   122281 tcatctatta gtatagaatc tcctgattta acgtcttcag caagaccttt atagcttact
   122341 gtacacattt ctttagtccc cataatatct ttcatagtta tagtgaactt ttgcccttct
   122401 tctaaaaata cctctgggtc ttcaaagtta cctgttctaa tttctggacc tttagtatct
   122461 aataatatag caactggctt atttaatttt tctctaactt tctttgccat atcaattctt
   122521 tctttatgtt cttcatgcga accgtgagaa aagttaaatc tacacacatt tagaccattt
   122581 tgcataagtt gagttaaaat ttcctcactt tgtgatgctg gacctagtgt acaaacaatt
   122641 tttgtcttct ttacattatt taacatattt gctcctcctg agatattatt ccacagattt
   122701 atttttaata ctaaaactta tatagataat atttttgcca tctcataagc ttctttatca
   122761 aatactttct tttgagctaa agcttcactt atttctaaat caattatttt gttttctttt
   122821 attcctacaa ctctagctga ttttccatct aataataatt ctactgctct tactccaagt
   122881 ctacttgcta gtattctatc agatacagtt gggcttcctc ctctttggac atgccccaat
   122941 acagtgactc ttaaatctgc tcctgactct tttttaagtt ccttttctaa atcactagca
   123001 cttccgactc cttctgctaa aacgattata ctatgtctct tacctctttt ttgagtagtc
   123061 tttaatctta gagcaacttc atctactgta atttctactt ctggtactat tatagtttcc
   123121 gctcctcctg caagacctgc atataaagct aaatctccac agtgtcttcc cataacttct
   123181 actatgttaa ctctttcatg agatgatgat gtatctctta tcttacctat agcatctata
   123241 atagtattca ttgcagtatc aaatcctata gtataatctg tatatgctaa atcgttatct
   123301 atagttcctg gtatgcctat agctgggaat cctaagtcac taagtttttg ggctcctgca
   123361 aatgaaccat ctccaccaat tacaactaag caatcaattt tatacttttt aagaattttt
   123421 actgctttta atctaccttc ttctgtttta aattcttcac atctggaaga ttttaatata
   123481 gttcctcccc tgtgtataat atctccaaca gaagataagt tcatttcagt taaatcttct
   123541 tctagtaatc ccttatatcc tctattgatt ccatatactt tgcatccata atatattgct
   123601 gatcttacaa ccgctctaat cgcagcattc attcctggag catctcctcc acttgttagt
   123661 aatcctatag ttttcatttt ttacctcctt ggattttatg tcaatttttt attataatca
   123721 ttgttattgt attctaatgg actttttttg tccctatatc actttttaat cccatcaaga
   123781 ctaaaaaaat taaatgtcct taaatgtcca ttttgtgata ttatatcaca aaacggacaa
   123841 tatttttcat attattataa tatatttaga tttttatata aaaaatcaag cttttacttt
   123901 tatgttatct ttaggaagta actcttctaa gttatttata aattcatcac atatacaagt
   123961 caatgccccg gtcattttat atgctttacg tttaccattt atattttcat ccatagggta
   124021 caagtatata ctgtcattac ctggatattg tttggctatt tctacaatat tattagttgt
   124081 atcagtatca gacatactat ctattttcaa atacaatttt ttaccacttt ctggcttaat
   124141 attttctaca tttctagttc ttcctgaatt gtagtttcta tctgcaaagt cagtatcatc
   124201 atttatatct tttatctctc tagctataag ttttgcattt tcgccttctt ttatacttaa
   124261 tgttcctttt acatatatta tattatcttc attcaaaatg acattatact tttgtaataa
   124321 ttgaggaaaa actattattt ctatagcacc atacaaatct tctaattcaa taaatgccat
   124381 aatttcattt cgttttgttg ttttaatagt tttagttaca atcattccac ccattataac
   124441 atctgtttca ttcatttgta ggtatgactc ttcttcctct tttaatgaat ttaattttcc
   124501 attatccata gatgtttttt cttgtagttc tttttcaaat tgtgataaag gatgtccact
   124561 tacatacatt cccaagactt ctttttcaag gttaagtctc tctctttctt catactcttg
   124621 ccttttaggt aaactgtata tttgttgaaa atcactatct tcctcactat ctccaaaagc
   124681 atcaaataaa gaaatttgac cttgaacatt tttctttcta tccatagata cactttctaa
   124741 taaactttca taaccagcca tcagactagc tctattttca cttatattat caaaagctcc
   124801 acattttatt agactttcta ttactctctt atttgtatct ttttggtcta atcttttgac
   124861 taaatctact aaatctacaa ataatccatt agattctctt tcagcgatta tattgtttat
   124921 tatatttaca ccaacatttt ttacagctgc tagaccaaat cttatattgt cgccttcaac
   124981 tgaaaacttt gaaaaacttt tgtttatatc tggaggtaat acatctattt ctagtgcttt
   125041 acattctctt atatactcaa ctaccttatc agtatttccc attacactgg ttatcaatgc
   125101 tgccataaat tcaactggat aatatgtttt tagataagct gtctcatatg ctaaaacccc
   125161 atatgcagct gcatgtgatt tattaaatgc atatctagcg aagtctatca tatcatcaaa
   125221 tattttatta gctatttcct cttctacacc atttctgata caccctgcaa tttctatatt
   125281 accatcttca tcaaatttac catgaataaa atattgtctt tcttcttcca tcacgtccat
   125341 ctttttctta ctcatggctc ttctaactag gtcacttcgt ccgtaactat aaccacctaa
   125401 atctctaacg acctgcataa cctgctcttg atataccaaa caaccatatg taacttccat
   125461 tataggcttt aatttttcat gtagatatgt tacatctttt gggttgcttt tattttttat
   125521 atatgcaggt atagaatcca ttggacctgg tctaaatagg gatattcccg ctacaatatc
   125581 ttcaaaattg tctggtttta gctgtttcat aaaacttctc atgccagcac tctccaattg
   125641 aaacacccct agtgtatttc ctgatgaaag catttcatat actttcgggt cgtcataatc
   125701 catctttgaa aagtctattt tttctgtgta gttttttata actctatttt tttctattaa
   125761 atcaagagta tctcttataa ctgtaagagt tcttagccca agaaaatcca tctttaaaag
   125821 cccaagttct tcaagtgttg tcatagtaaa ctgtgttgtg attgcatctt gatgtttgta
   125881 caaaggtaca tattcatcta cgggattttt tgatataaca actcctgctg catgagtaga
   125941 agcatgtctt agcattccct ctatctgttt tgatatattt attatttctt ttgtttccgg
   126001 gtcttggtca tacaattctt taagattagg atttgtatct agggccttat ctattgtcat
   126061 tcctaaagca aatggtattt ccttggctac cttgtcaact ttattgtagc ctatatccaa
   126121 tacccttcca acatctctta ttgcagcttt tgcacccatt gttccaaaag ttattatctg
   126181 ggctacatgg tcatcaccat attttctctt tacataatct ataacttctt ctcttctctc
   126241 ataacaaaaa tctatatcta tatctggcat ggatatacgt tctggattta aaaaacgctc
   126301 aaataataga gagtacttta ttgggtctat atcagttatc ttaagtgtat atgctactat
   126361 cgaaccagcc gcactacctc tacctggtcc tactataata ttattttctt tagaaaaatt
   126421 tataaaatcc caaacaatta aaaaatactc tacataaccc attttttcaa ttacatctaa
   126481 ttcataattt aatctctcta gtatctcatt gctaggatta tcatatctct cttttaatcc
   126541 attaaaacac aactccctta aataagtttc tggtgtatat ccttctggaa catcatactt
   126601 aggaagatgt atagtattaa aatcaaactc cacattacac atatcagcta ttttcacagt
   126661 attgtcaata gcctctaatg cgtatggaaa taattcttcc atttcttctc ttgattttaa
   126721 gtaaaactca tcactaccaa atctcattct atttgggtca tttaatgttt ttcccatttg
   126781 aatacacata agaacatcat gtattttcga atcttcttta tttacatagt gaacatcatt
   126841 agtagcaact agtggtatac cagtctcttt tgataatttt actaaaccag agtttacttc
   126901 tttttgctca ggtaaattgt gatcttgaat ctccaaaaag aaattttctt ctccaaatat
   126961 gtctctgtag tcaagagcaa attgttttgc tttttcataa tttctattca ttaaagcacg
   127021 tgctacatct cctgcaaggc atgcagaaag tgctataatc cctttgttat gttttttaag
   127081 ttcttccatg tcaactctag gcttatagta aaaaccttct acgtatgaag ttgaaactat
   127141 ttttattaaa tttttatatc cttccatatt cttagctaac agaactaaat gtccttgata
   127201 tttgtcatag tttgggtctt tatctctcaa tccacgtgca gctgtataga cttcgcaccc
   127261 tataattggc tttattcctt cttttatagc tgttttgtag aaatctataa caccaaacat
   127321 acaaccatgg tcagttatag ctattgagct catgtttagt tccttagccc tttttataag
   127381 ttttttcacc ctcgaaaacc catcaagtaa actatactca gtatgtacat gaagatgagc
   127441 aaaatctttg ttcattaaat cacctcctgc tttataattt taattttacc acaaatatat
   127501 gtcctaaaac aaaaacagag acttttatct caaaatagga attaacttca acatatacca
   127561 ttatatgtta taaattaatg cttattctga gattgtcccc tcaatttatt gctatgattt
   127621 ttttttcaaa tctgttgcaa tttcttctat ttttctaagt ctatgattga ctccagattt
   127681 tcctattggt ggttctaaca tctctccaag ttctttgaga gtcatatcct catactcgat
   127741 tctaatctta gctatctctt gtaaattttc tggcaaactg ctaattccta tagtctcttg
   127801 tatgagctta atattttcta cttgtcttac tgctgcattt actgttttag aaaggttagc
   127861 tgtctcacaa tttactatcc tgtttacatt atttctcatt tcttttacta ttttagtatt
   127921 ttgaagactt aaaagagcgt ggtgtcctcc tataatactc aacaaatctg atatttgttc
   127981 actctctttg atataaacaa cgtgattatt ttttctagcc acaattttac tattaaaacc
   128041 taaagagttt ataagttctt ttagagaatc tgcaaactcc tcattattag ttacaaattc
   128101 catatgatag ttcttttctg ggtcgcttat agaacctcca ccaagaaaag ctcctcttat
   128161 gaaagctctc ttacatcctt catgcttaat taagttctct gaaattttgt ttatagtaaa
   128221 aaagccttct ttatcttcaa gtatacctaa cttttttaat aacatttctg aacccatttc
   128281 gcttgttacc attaatacat aactgttatt tctctttagc atttgattct tatttactga
   128341 gattgtagta ttgatattaa aattcttttt taataattta aatactttac gggctattga
   128401 atttaactct gttgttatct ttagatttag cttcttatat ccaacgattt gtatacttcc
   128461 ccctgattta actagagctg ataattcagc tatattacaa cattcattcc cagatataac
   128521 tcttgctagt tcatttttgg tttcagttga aaaagacata tctatcacct attaactaat
   128581 ttttctttaa cctttttttt atttcttgta aggcaacctt tattatctcc ttttcatcat
   128641 catacctagc taggtctacc actctatcta gatggataaa tttagcatca ataaagcctt
   128701 cctctttttg tggaatagta tccatattct ttgcttgcat caaataccaa aaaactgttt
   128761 tatgaactgc tctattttca tcccagtttt ctttgaatgt atagtgtatt tcacctaaat
   128821 atttcaatat atctgctttt actccagttt cttcacttac ttcacgtaat gctgcctctt
   128881 ggttattttc cccttcttct acttttccct tgggtaacac ccaatctcca ttgaacttcc
   128941 ttagtaaaag aatcgtattg ccaaacagga ctacaccacc agcactaacc tcttctctca
   129001 tcgatgttca ctccttaaaa ctaacataat gataaggatt tctacacaat tttttaaaat
   129061 ccttttttac taacttttgt tgtaattatg atttagggct aactcaataa ctatgttaga
   129121 tatatacttt gcatcatgtc ttatataatt attttttatt tcaattaaat tcttctctac
   129181 ggtttttata cccatctctt ttaatttatc tttttgcttt ttatctaaca agacttgctc
   129241 tgctccatct tgtttatata agtcaagcat ttcttctggc aatatctcat tatttgcgat
   129301 tacataatct attaaattct catcagtgtg ctttactata gcattaacat gctctaatac
   129361 attgtaccct tctgtctcac ctggttgagt cattatattt gctatatata cttttggagc
   129421 tacagacgat ttaatagcgt ctacaatccc ctctacaagt aaattgggta ttatactcgt
   129481 atataagctt ccagggccca taattataat gtcagcatcg taaatagatg caataacatc
   129541 ctttaatggt ttgacatctt ttggatttaa aaaaatttta tcaatagagc ttttttgaat
   129601 ttttgactct tcaggtatac tagattctcc atttattata ttgccatttt ctaatttggc
   129661 aactaaattt acatcttcca aagttactgg taaaactctt cctgttatag caaaaatttc
   129721 acttaattta tatacagctt tttcaaagtt tccatacaac ccattcatag cagccaaaaa
   129781 caaatttcca aaactctgac cttttaaaag cccttctgta aacctatatt gcataacttc
   129841 attcattgtt ggctctatat ttgcaagtgc taatagacag tttcttatgt ctcctggtgg
   129901 gagcattcct aagtcttctc tcaatacacc tgaacctcca ccatcatcag caacagttac
   129961 aattgcagtt atattttgcg tagtgtgttt aagacctctt aaaaatactg attgaccagt
   130021 acctccacct atcacaacca catttggttc tctattttca acaatagacc tttgaaatag
   130081 ttttactttt tcattttttg cttttttata tataaataac gatacaatta atgcaatcca
   130141 acctaaaatt cctacaataa taagtaattt agccatgttt gcctccatta tcgcaacata
   130201 gagtctctat gtttctttac aactctatat cccttattag ataaatcctc cgctataaga
   130261 tttgttatag taactgacct atgtcttcca cctgtgcatc caacaccaat tactagatgt
   130321 tgttttcctt catctatata ttgaggtact aaaaaatgaa tcatatctaa aagtttcaca
   130381 taaaattctt cactgatttt agaattcata acataatctc taacttcttt atcatcacca
   130441 gttttagctc ttaactcttc aacataatag ggatttggaa gaaatcttac atcaaatact
   130501 aaatctgcat cagcaagtat accatgttta aatccaaaag atactacaga aatagttagg
   130561 tttggattat cttcaccaga agaatatatt ttctttattt cttccttaag gtctttaggt
   130621 ttcatgttag ttgtatcaat tatacatgta gataactcct ttaaaggttc cattatcttt
   130681 ctttccattt ttataccttc tggaatctgc atatcttttg ccaatggatg atttcttctt
   130741 gtcattttat atctctttaa caatacatca tcattacagt ccaaatatac catttcatat
   130801 tcataatttt ctttttctaa ataatttaag ctttcatgaa gcgcttcaaa aaactttctc
   130861 cctcttatat ctatccctag agcaacttta tcaatacttg aatttggttg ataacataat
   130921 tctgcaaatt tagttatcaa agcaggaggt agattatcta cacaataaaa tcccatatcc
   130981 tctaaagctc tcattgtctc actttttcca gatcctgaaa gacctgtaac tataacaaat
   131041 ttcattgcta actcctccct tttatcttat gtttattata ttataatgtt ttctactaac
   131101 gatatatcca gattagagtc agatatagtc ttcatagcta gtttaaaatc accaactctt
   131161 tctggacaat gtgcatctga gcctattata aatttatttt ctagcccttt tattttattt
   131221 aattgattta cattcaaaaa agaatggcta ctatttattt caagtctagt atttgtcttt
   131281 tttgctgtct ttgctacttc ttctatatac acatcgcctt tatctcctgg atgagtaatt
   131341 ataaaaatat cattattact tatcgcattt attacagctt ttgtattgta ttcttttgct
   131401 ttttcagttt taaaaatata gttattacaa tgatttaata agcttcttaa tgatgttgga
   131461 gtagaaccaa aatgatatcc agccataata taatcaaaat ctctaataaa tttatcatcc
   131521 atatctatat tgcctttatc atctaatatg ttacactcca tacctaaaag tatatttata
   131581 tcattgtact tatcatttaa ataatctact tcttctctca ttttgagaat atcttttttc
   131641 tttacaccat atgcaatatg tttatagcta tggtcagata ttcctattgt ttttattcct
   131701 ttagaaatgg ctacctttac attatcttca atagtccctt tgccatgact atataatgta
   131761 tgtgtatgat aatcagccag tattttcatt tgttaatcct ctccaagtat tttgacttct
   131821 tcttccaaca taaccccaaa cttggcatat acagcacttt ttataacata tattaaatcc
   131881 aaaatatctt tagcacttgc ttctccttga ttgactacaa atccacaatg cttctcagat
   131941 acttgtgctc ctcttaaagt aagacctctc aatccagttt cttctattaa tttacctgca
   132001 aaatatcctg ttggtctctt aaatgtactt cctgcactcg caaaatttaa tggctgttta
   132061 gttattcttc ttgtcgctaa atcatccatt aaacttttta tttcatcata atttccttct
   132121 tgtaattcta gttctgctga taaagctata tatccgtttt tcgatattat actttttcta
   132181 tacccaaatt ccatctgttc atttgataat tcaaatatat tgccttccat atccatcagt
   132241 cttactgact tcactatgtc cttcatttca ccaccatatg ctccagcatt catggctagt
   132301 gctcctccca atgtaccagg tattcctgat gcaaactcaa acccttttaa ttcttgcttt
   132361 tggagagctt tacctaaaac tgataataaa gctcctgact gagcagtcat cctagtatca
   132421 tttatctcat aagaattgaa attatcagct aattctatta ccactcctct tattccacca
   132481 tctttaatta aaatgtttga accatttcca attattaaat acggaataga ttcttctttt
   132541 accaacttaa gtacattttt taattgttct tcagttctag gtctaactaa tatatctgct
   132601 ggtcctccta ccctaaatga aatatgtttt ttcataggtt catcgacttt tatatcctct
   132661 ttacttaata tatttaataa atttatatat atatcttgat tattcattgt atttcctccc
   132721 aaattatact gaactttagc tgtcattatt atttaaatta cattagtata tagtattaac
   132781 tatattcaat aatatatgat tttgcttatc atatggattt tatattttaa gcttcttaat
   132841 tacaaactat attcaataaa tatataataa agtaaaaaaa aaaaaataac aacatataaa
   132901 agaatataaa taatttaaaa aaagcatcaa tatcactgat atcaatgctt taaaaggcaa
   132961 tttaattcgt attatttatc taactatttt tgacatagca aagataataa atccctccaa
   133021 ctactatgga acctccaacg atatttccaa gcgttacaaa taccaaatta gttataaatt
   133081 gaaccatagt tatatttgct cctaatgcca ttcccattgg tataaaaaac atgtttgcaa
   133141 cactatgttg gaatccgcat aatacaaata acattattac tgtccaacat gaagccaatt
   133201 ttgacattat atcttgagat gctgtagcca tccatacagc taaaacgact aaaatattac
   133261 aaagtattgc ttttaaaaac attgatgcta tagttgaatt aaccttagct tctgccacag
   133321 ctatagcttt tgtagctgca tctcctgtca atgtagaaga ataaaacaca agtgcaacta
   133381 gcagaattga acctactaaa tttgcaaaat aaaccagtcc ccagtttctt aacatttttc
   133441 ctaatgttat ttttttgtcc ataagtgcaa tagccattaa ggtgtttcca gtgaataatt
   133501 ctgctcctgc caccactaca agcataagac ctactggaaa gaccatagca cttaaaaagc
   133561 tagctacccc tgggtctatc ttgccaaaag tttgactaac tatcatactc cccaatgccc
   133621 caaaacctat aaatgctcct ccaaataaac ctaatagaat tgcattttgt gtcgaaatgt
   133681 ttgctttttt aatgcctgca cttatagttg cttgtgcagt ttccccaggt gtcaagaatc
   133741 ctttactcat aaatccttcc cccttaaaaa ttatactttc ccttgtcttt ttatggcaat
   133801 aattatataa taacaatatg atatataata aattttatta acataaaaaa tatactatat
   133861 acttatatat acttgtctca tataattata ctatttatta tataacaact ttgttaattt
   133921 cgaaataatt tatattgaat ttgaaaaatt taccttttga ttatttattt tactttttaa
   133981 ttattgaggt attatatata atagatatat gtttgtgtta ttatatataa ttacttattt
   134041 taataaatat ttgaaggagg gaattttatg tttgatatat catttttcga aatgtcaata
   134101 tatgaaatag cagttactac aatatatata attaatttca tggttatact aaaccttatc
   134161 tttagagaaa aaagaaacat aaatacaact ttaacatggc ttcttatatt agtattgatt
   134221 cctgctcttg gattcatact ttatatagct tttggcagaa atatttcaaa aaacaatatg
   134281 tttagattga aagaaaagga tgataagata atcaaaagta atatactaga tactcaggtt
   134341 aaactccaat ctacatctga aattgattca gatatacatc aacataagga tatgatttat
   134401 gcattggcta attcaaataa tgctcattat accaataata acgatgtgtg gatttatgct
   134461 gaatctagtc aattttttaa cagtcttctt gaagaactta aaaaagctaa aaaatatata
   134521 aacatacaat tttacatttt caaagatgac aaaatcggaa ctgaaataat agacattctt
   134581 atagacaaag ctaaagaagg tgttgaagta agacttttat ttgatgcagt tggtggtaga
   134641 acactaaaaa atagtacact ttccagactt aaagaaagtg gtgttaaagt tggaagcttc
   134701 ttcccatcat tcttgaaaat tgtaaacttt aacttaaact atagaaatca ccgaaaaatt
   134761 gttgtcattg atggtaaggt aggatttgta ggtggatata atgttggtga tgaatattta
   134821 ggtagaaatc caaaatttgg tctatggaga gatacacata ctaagcttac tggtgattgc
   134881 gtaattgacc taaatatgag attcatcctt gattggagat atacaactaa agaagattta
   134941 gatttagaaa agtactttat agaagaagat acaaattcta atcatccttc tgaaaatatt
   135001 gggattcaga tagtatcaag tggtccagat atatcagaac ttgatgagat taaatatggt
   135061 taccttaaaa tgattcagaa agctagaaag tatatataca tacagagtcc ttatttaata
   135121 cttgactcaa cttttataga tactttaaaa atagcttgtt tatctggtgt tgatgtgaga
   135181 gttatgattc cttctaaacc tgaccatccc tttgtatact gggcatcata ttcatatgct
   135241 ggagaacttt taaagtttgg agctaaaatc tacacttatg gtcaaaatgc atttttacat
   135301 gctaagacaa tagttataga tgactctata tgctctatag gaacagctaa tatggatatc
   135361 agaagttttg agcttaattt cgaagttaat gcatttatgt actcttcaaa aaaagcattg
   135421 gaacaaagaa ttatatttga aaatgatatt ttaaattcta aggaaataac attagatata
   135481 tataattcta ggtctactta tataaaaatc aaggaatcaa tttcaagact attatcagca
   135541 gttttataaa tcttttattt tataaaaatt ttacattttg gtttattttt taaagttttt
   135601 tatatgtatt attttttttt tattgaattt agctcatttt ttttattttt tttaactatt
   135661 ctgaattatt ttatattata atagactagt atagttttta tttttaatag gtaacttttt
   135721 tgcattgttt ttataaataa agaaattgtt gtatattatt ctttctttat tgttctggtt
   135781 ttgttcaccc acataatgtt ggggtcaact catggttaat tattttatga aaaataataa
   135841 ctttaaaaag aaaaggggga tttgttatgt gcgattttat ttcacacaac aagcaattat
   135901 ttgacgaact agatatattt attgactctc ttacaacaaa ggaaggtgcg ttaatacaag
   135961 ttctacatga agcccaaggt atatttggat atcttccaaa agaagttcaa cttcatgttg
   136021 ctagaaaact tggagtagct cctgcaaagg tatatggggt tgttacattc tattcttatt
   136081 ttactacaga acctgtaggt aagtataaaa tcagtgtctg tctaggaact gtatgttttg
   136141 taaaaggtgc tgacaaaatc ttaagtgctt ttgaaaaaca gcttggcata aaagttggag
   136201 aaactactag tgattttaaa ttttctttag agggtttaag atgtttgggg gcatgtggtt
   136261 tagctccagt tgttactgta aatggtaaag tttatggtaa agtgaaacct gaccaagtaa
   136321 gcgaaatatt agatacttat agagaattag aattgaattg ttaagagggg tgaataaata
   136381 atgcgtaaag taaattcatt tgatgaatta aaagttttag ctgatgagct gaaacctaat
   136441 cttagcttaa gaaaaaatta ttctaaggaa aatgtattaa gaagagaatt acttgtttgt
   136501 tgtgatactg gatgtacctc ttcaaacagt ctagaaatag ttagtgaatt agaaaatgaa
   136561 ataaagaaat caggaatcca agataaagtt agtgttcgtt tgacaggttg ttttggtttc
   136621 tgtgctcaag gtcctattgt aaaagtttat cctgataacg ttttctacgt aaaagttgag
   136681 ccatctgatg ctgaaaagat agttcaaagc cacttaatta gaaatacagt tgtcgaggaa
   136741 ttattatatg aggaagaatc tttaagtaaa aaagttgaag aacaagaaga aatgtctttc
   136801 tacaaaaaac agttacgtat agctttaaaa aattgtggtc ttatagaccc agatagtata
   136861 gaagaatata ttgccaatga tggttattta gctctaggca aatgtttaac ttctcttact
   136921 cctcaagaag ttattgcaga ggtaaaaact tctggccttc gtggtagagg tggtgcaggt
   136981 ttccctactg gctctaaatg ggaagctgct tcaaaaaatc cagctggtcc aacagataaa
   137041 aagtttgttg tatgtaatgc tgatgaaggt gacccaggtg catttatgga tcgatctgta
   137101 cttgaaggtg accctcatag tgtactagaa gctatggcaa tatgtggata cgctataggt
   137161 tctgatacag gatacatata cataagagca gaatatccaa aatctataga aagattaaaa
   137221 gtagccatag ctcaagctga aaaatacggt ttacttggcg aaaatatctt aggcactgga
   137281 tttaacttta aattagaatt aaaatatggt gcaggtgctt tcgtttgtgg tgaaggtaca
   137341 gcgctaatgc attcaattga aggtagacgt ggtgaaccta gaatgaaaac ttatagttct
   137401 tctaagagtg gtctatggaa atctcctact tgcttaaata acgttgagac ttttgctaac
   137461 atacctgcaa ttatacttaa gggtggagat tggtttgcca acttaggtac tgaagattct
   137521 agtggtacta aagtctttgc tcttggtgga aaagtagaaa atgtaggact tgttgaagtt
   137581 ccaatgggta ctactttaag agatattgta tttgaaattg gtgggggaat ccctgaaggt
   137641 aagaaattta aggctgttca aacaggtgga ccatctggtg gatgtatacc aactgaacat
   137701 ttagacactc ctatagactt tggttcatta agttctatcg gttcaatgat gggctctggt
   137761 ggtatgcttg ttcttgatga atctgactgt atggtagata tagccaagtt cttccttgag
   137821 tttactgttg aagaatcttg tggtaaatgt acaccttgtc gtataggtac tacaagatta
   137881 ttagaaatat taactaaaat tacagaaggt aatggtactc tacaagattt agatgaccta
   137941 gaaaaccttg ctgaaactat acaaactgct tcattatgtg gtcttggtaa agctgctcca
   138001 aaccctgttt taagtactct tcaatatttc aaagatgagt atatagctca tgttgtagat
   138061 aagaaatgtc ctgctggtaa atgtcagaac ttactttctt actttataac agatgactgt
   138121 aagggttgta caaaatgttc tagagtgtgt cctgctggtt gtataacagg ttctgttaag
   138181 gaacaacata caatagatac ttctaagtgt ctaaaatgtg gtgcttgtat agataattgt
   138241 acatttaacg ctataatcaa gaaataactt atttggagga ggaataacaa tgagtttagt
   138301 taatttaact attaatggca aacacgtttc agcacctagt ggaacttcta tcttagatgc
   138361 tgcgaaatta ataaatataa aaatacacaa cctttgtcat ttacatatga acgaaataga
   138421 taagcttgac acttgtgctt cttgtcgtat ttgtatggtt gagacagaga gaggcttagt
   138481 tccagcttgt ggaacagtta taaaggaagg tatgaaagtt cagactaata gtgctaaagc
   138541 attaaatgct cgtagaacta ttgttgaatt attgttatca gaccatccac aagattgttt
   138601 tgtctgtgaa aaaaatggag attgtgaatt acagactata gctgctgatt taggagtaag
   138661 aaaaataaga tatcaaggtt ccaaatcttt tgcaggtaaa gatacttcta ctaaatcttt
   138721 agttaaagac cattctaagt gtatattatg tagaagatgt gagactgtat gtaatgacat
   138781 acaaacagta ggagcattat ctggagttaa tcgtggattt aatactttag ttagtacatt
   138841 ctttaactct gatatggttg aaacagagtg tacattctgt ggtcaatgta tatctgtctg
   138901 tccaactgga gcattaacag aagtagataa tgtacctaaa ctttgggatg ttttaaataa
   138961 aaaagaaaag actatagtag tgcaagttgc tcctgctgta agagttgcta ttggtgaaga
   139021 gtttggatta gaaccagggt caatctctac aggaaaaatg gtagctgctt taaaagcact
   139081 tggatttgaa catgtatttg atacaaactt tggtgcagat tttactataa tggaagaagc
   139141 tactgaattt atagaaagaa tccaaaaagg tgaaaatctt cctatattaa caagttgttg
   139201 tcctgcatgg gtaaacttct tagaacacaa ttatccagac aagttaaact tagcatctag
   139261 ctgtaagtct cctcaaggta tgtttggttc tatagctaaa aactactatg cacctaaaat
   139321 attaggaatc aatcctgaag aactttatgt tgtatctgta atgccttgtg tggctaaaaa
   139381 atatgaagct tcaagagaag aattaagtga atctggaata ctagatgttg acctttctat
   139441 aactactaga gagttagcta aaatgataaa agaagctgct atcgatttac ctaatcttca
   139501 agaccaagat tttgataatc ctctaggaaa atctacaggt gctgcatcta tatttggagc
   139561 aagtggtgga gttttagaag ctgccctacg tacttcatat gagaagataa ctaataagac
   139621 tttagataat gtaaacttca ctaatgttag aggtcttaaa ggaattaggg aagcaagtat
   139681 agatgttgat ggaactacag ttaatgtgtg tattgtaaat acacttaaaa atgctagaaa
   139741 gattatggat aaggtcagaa gtggagaatg taaataccat atcatagaag ttatggcatg
   139801 tcctggtgga tgtgttggag gtgctggtca accttatcac catggtaata ctgaaatagt
   139861 tgatagacgt gcaaatgcgt tatatgaaat agatagaaat aaggctattc gtaaatctca
   139921 tgagaatcct gatttacaag ctatttataa agacttcttt ggagaaccaa atagtgatgt
   139981 agcccataaa taccttcata ctcattactt tgataagagt tgtgtctatg gagagtgtcc
   140041 acaagaatgt gcttgtgaag aagctaagta gtcataacaa agtataaaat ttattcttaa
   140101 aaaaggattg tcttaaaata cttttacgta atttaagaca atccttatta atttcacaaa
   140161 ttaaattata tttctaaata gacttattct ttaattaacc tatctattaa ttaaagaata
   140221 cctgccattc tcataagttg ttttgcattt gtagttttac atcttccatc ttttatctta
   140281 tagtcaaaat gaattttatc gtctacatag tactctgaga aatgatagtt tacaacttca
   140341 acatctccac tacctgaaag gtcacatagc tcaaagtcat gtgttgtcac catacttata
   140401 acccagggtt ttgataattt ctttactgcc tcacttgctc caataattct atcagcagag
   140461 ttagttccct taaaaatttc atctactaga accaacattg gcagttcttt taaactatat
   140521 tgaatcattg attttattct aagaatctca gcatagaaag ttgatattcc ttgacttaca
   140581 tcatcttgta ttctcataga agtaaatata gccatacagg tcgcatcaaa actcttagcg
   140641 catactggag cacctgcata tgccagcact aagtttacac cgatacttct taaaaatgtt
   140701 gtctttccag acatatttga acctgtgata atacaagtct gagaatttaa ttgtatcgaa
   140761 tttgctatga cacttttttc tgctattaat gggtggtata cctcttcaac tttcaatctt
   140821 ggagtatctt catattttat tactggaaaa catgtatttt cctttacatg agaaataact
   140881 gttaaactta ttagagcttc aaattctcca atagattcaa accaatctct aatttgattt
   140941 ccatatatac ctttccatcg ctccaaagct tctgcacaat gatagttcca catcaaaaca
   141001 ccacaggcaa ttatatatag tatctgatta taccttaggt ttacagcatt tcctattgaa
   141061 tttaattgtt taacacctct tgaaactcca cttcccttag aaagtttatc ttgtaattct
   141121 ttcaaatatg cactctcaaa cgtctctttt tctaaaacct caaacatttt ttcatatact
   141181 tgtatacttc tactaaatga aaataatgga gaaagagttt gcataagtct tggatatcca
   141241 aaaccactaa accctaactg gattataaaa agtaaaaaaa caggtaaaac aggtaaaaat
   141301 cctaacatac aaaatataaa agataagatt gttgcagttg gtagacccca agtaaaaata
   141361 tgcatccatt ttggtatata cacattctta ttttctccat actctataaa cgattctata
   141421 ctactatctg aattattttt ttgctcttta ccaattatat tactgagtat ttgaatatga
   141481 atagaaaaat catcttttga taaaagttct tttatagccc tctgtctttc taagattaca
   141541 ttctcatctg gattttcatt ccacaaatat ttggctaaaa aatttttccc ataagatgta
   141601 ttagctacac agatatattg atacaatgat gctcttccaa ataagtctaa atcttttgct
   141661 tgcgaatttt catcttttaa gtattcttta ccatctattt taaattcctt ccaaccatct
   141721 ccaaatctct taatatactt atttaatact tcgctcttac ttttaaaata tgtttcttct
   141781 tcttttactt tactatgaat aactaagagt gctataaata ttattattga taaaactcca
   141841 acaaaaatac ctactatatt tttattgtaa aatccaatag caaaccctgc tatcactatc
   141901 aaaaaagata ttaatcttgc tgtacttatg ttatttgact ttttacttaa gagcaattct
   141961 ttttcagtac tactttctaa aagtttacta aaatattcta ttcccataaa aaattccaac
   142021 tttcttgtta tataatgaaa ctcaaatata aaattaatct agtattatta taatctaaca
   142081 ctgtacttta aattatcctt atataatata tcttattaat aaacattata tatatacaac
   142141 ttgattttaa tttataaggt gtaggacata atcctaatca atatttaatt aaatgatgtg
   142201 tagttcacta ttattcagta ctacttagta tcgattaatt ttgataattt tttagtaaat
   142261 tcttgagctg catcgtatcc cattcctctt tgtctaaaat tcattgctgc tacttctaat
   142321 atcatagcag tatttcttcc aggtttaaca ggtacaacta atttctctac attaacacca
   142381 agtatttctt cgtattcttt atccagtcct agtctatcat aatacttagt ttcatcccaa
   142441 gcttctaaat gaactactaa atctatcatt ttagaatttt tgattgcccc tactccatat
   142501 aaacttctaa catctattat tcctattcct cttatctcaa gaaaatgtct tattagttca
   142561 ggtgactgac ccacaagcat actttcatct aattttctta tttctacagc atcatctgca
   142621 actaatctat tacccctttg aatcagttct aatgctgttt ccgactttcc tatactactt
   142681 tcacctttta tcagtacacc tataccataa atatctacta gcaccccgtg aattgttgtg
   142741 tgtggtgcta acttattatc caaatagttt gacagtctat ttataagtct agtggtattt
   142801 ctatttgtgc tcaaaagtat tcttttatat tttttagcct tttcaattac atctggcttg
   142861 atttccaagt ctctagaaat tattaacgct ggtatttcat atgagaaaaa tctatctagt
   142921 acttcttctc gaatatgact atccattaat ccaaaaaaat tatattctgt ctttccaact
   142981 atttgtattc tttcatatgc aaaataatca aaatatcctg caaactgtaa tcctggtcta
   143041 tttatatctt gagagtagac ataataatct acatcatcag gcatgtatac tacttttaac
   143101 ttcaactctt ttattatctc tcttattgat actttattgc tagtttccat taattttctt
   143161 cctttctaaa aaaattataa acattatctg caatgctttt attcattcca tctacttcta
   143221 aaagttcttc catagtagct ttttttatag cctctacatc tttaaagtga tttaataatg
   143281 atttctttct tttttcacct attccttgta tatcatctaa gatagatttt gtcaaagctt
   143341 tatttcttag acttctatga tatgttattg cataattatg aacttcttct tgtatactag
   143401 caataaatct atataaatta gttgttttat ctagttctat ttctttttct ttgcatatca
   143461 atcctttagt tctatgctta tcatctttgt acattcccca aagaggtata tctacatcat
   143521 tgagttctag tacttttttt actgcactaa cttgtccttt tccaccatcc aacaatatca
   143581 agtcaggtaa atttccatgt tttaatcttc tatctacaat ttcagccata gaatcataat
   143641 cattaggtcc aataactgtc tttattttat atctcctata ttccttctta tcttttttag
   143701 cattcgtata cactaccata gaaccaatcg aatctacacc ttgtatattt gatatatcat
   143761 aagcctctat tcttattggc aacttttcta gatttaatat ttgttttaat tcttccaatg
   143821 ctccaatact tttttcatat tttcttttat tcatatcaga aaatttttca agatattcta
   143881 cagcattttt cctaaccatt tcaactaagc ttttcttctc tcccttttga ggaactctta
   143941 ttgttacttt ttgccccttt ttagatgaca gccattcttc taatatagaa ctatcttcta
   144001 tctcatcttc tataattaat tctttaggta tatactcttg ttcattataa aattgtttta
   144061 caaatgaact taatatagaa gctcttggac tatccattac accttctaat ataaaatgtt
   144121 ccctacctac aatcttacca tttcttataa aaaatacctg gacacaagct tcattatgag
   144181 ctcttgccat tgcaaccaca tcttggttta agtcacttac agtagcatct attttttgtt
   144241 tctgtatcat ctcttcaagg ctctttatct tatccctata tacagcagcc tcttcaaatc
   144301 taaagttcat agaactctca ttcatttttt cctttaaaag ttccatcaat ttttcttctt
   144361 taccagataa acacattata atctcttcta tcatttttcc atactcttct ttagaaacat
   144421 ttcctgtaca aggtcctaaa catttattta tgtgtagatt taaacaaggt cttgtcttat
   144481 tctttattgc tttatctata tctattttac agcttcttat tggatacgta ctacttataa
   144541 gctccaaggt atcattcaca gcagttatat tggtataggg tccaaaatac tttgccttat
   144601 ctttaagaac tcttctaact tttaagattc ttggataatc ttcattcgta gttactttta
   144661 tatatggata agttttatca tctctaagca ctacattata ctttggtctg tatcttttta
   144721 ttaaattaca ttcaagtatt aatgcctcta actctgaatc cgttataata tactcaaact
   144781 tatatatatt cttaaccata gattttactt tagatgtatg gttttttgaa gattgaaaat
   144841 actgccttac cctatttttt agagatatgg cttttcccac atatattata tggtcgtatt
   144901 tgtctttcat taaatatact ccgggctcag aaggtaattt ttttaaatgc tcttgtatat
   144961 caaacatctc gccctccaaa acattaaatt ttatactaaa attataccct aaaatatgaa
   145021 ttttaaaaag ttatacaatt tccagtgctt tagacaagtt aaagtccata ttcccttcta
   145081 taattgattt gaaaacaatt ttaaccatga agagtaatat ggactttact ttgtattact
   145141 atttacaact tatatttgac taatttactt ataacaaaac agtttatcgt atatctgctc
   145201 taaaattttt cacaatttat ataacttaag aaaaatattt ctttaagaat tgacctgtat
   145261 aagaaccatc aactttagat acttcttttg gtgttccagt tgcaactata gttccaccct
   145321 tatctccgcc ttctggtcct aagtctatta cataatcaca agtctttata acatctaaat
   145381 tatgttctat gactataatt gtatttcctg tatctgctag tctttgtaat acttggatta
   145441 gcttgtctac atctgccata tgaagtccag tagtaggttc atctaatata tacaaagtct
   145501 ttcctgttgg tcttttacta agttcagcag ctaattttat acgttgcgct tctcctcctg
   145561 ataattgagt tgaaggctgt cctaatttaa tatatgaaag tcctacatcc attaaagttt
   145621 ctaattttct ttttatattt ggtatatttt cgaaaaactc aacagcttct tccacattca
   145681 tatctaatat atcagatata gttttatctt tgtattttac ttgcaatgtt tctctattgt
   145741 atctttcacc tttacaaatt tcacaaggaa catatacatc tggtaagaaa tgcatttcta
   145801 tttttattat tccatcaccc ttacaagctt cacatctacc accttttaca ttaaaactaa
   145861 atctaccttt tttataacct cttgctttag cttcattagt agatgcaaaa atatctctta
   145921 taaaatcaaa tacacctgta taagtagcag gatttgaacg aggtgttctt ccaataggac
   145981 tttggtctat atttataact ttatctatac tttcaattcc ttttatctcc ttatgcttac
   146041 cagctctttg cttaagtcta ttcactttac ttgcaacacc tttgtaaagt atatcgttta
   146101 ttaatgtact ttttccagaa cctgaaacac ctgttatgca tgtaaactta cctaatggga
   146161 actttacatc aatatttttc agattatttt ctgatgcttt aactatttct ataaaacttc
   146221 catttccttc tctagtagtc tcaggaagca atatttgttt tttaccactt aaatattgac
   146281 ccgttattga gttctcattt tcaattattt catctaaagt accctgtgca actatttcac
   146341 caccatgtat acctgcacct ggaccaatgt ctataacata atcagcttct ctcattgtgt
   146401 cttcatcatg ctctacaacg attaaggtat ttcctaaatc tgttagatgt ctaagagtag
   146461 ttataagcct atcattatct ctttggtgaa gccctatact tggctcatca agtacatata
   146521 aaactccaac caatgcagaa cctatttgag tagctaatct tatacgttgc gcttctcctc
   146581 ctgataaagt tccagctttt cttgataaat ttaaataatc aagacctaca tctcttaaga
   146641 acatagctct tccttttatc tcttttataa tctcatgagc tataaatctc tgtttttctg
   146701 taagatttat attttccata aagtcaatca attcatttac agataagttt gtaacttcca
   146761 ttatattttt tccatcaact aaaacagata aaacttcctt tttaagtctc atccctttac
   146821 actttggaca tggtctctct gccatgtatt cttcaatctt atctctcatg taatctgaat
   146881 tagtttctct atatcttctc tctaagttta ctattacacc ttcaaagtat gatttataat
   146941 ttctagttcc tccaaatttt gactcataag taaactctac aattatatta tctttaccat
   147001 aaagtaattc ctccacaaaa tcatctggta aatctttaaa cggtgttgtc aaagatacat
   147061 taaaatgctc tgctaaactt tttatcattt tactgtaata agtgtcatca ttaacacctg
   147121 ttgaacccca tgctgccact gcaccttgtt ttattgataa atctttgttt ggtactacta
   147181 attctgggtc aacttcttta ctttccccaa gaccattaca aacatcacaa gcaccaaagg
   147241 gagcattaaa tgaaaacatc cttggagata attcttctat gccaattcca tgttctgggc
   147301 atgcaaattt agttgaaaaa agaatttctt ctccatctat tatttgggct attacaagac
   147361 catcagatag cttaactgca gtttcaattg aatctgctag tcttgattct attccatctt
   147421 taattactat tctgtccact acaacttcta tattatgttt ttgattttta tttaagatta
   147481 tatcatcact aacttcatag ttctcaccat taactctaac tcttacaaat ccttcttttt
   147541 tgatattatc aaagactttt ttatgtgtac ctttttgtcc tctaacaatt ggtgaaagaa
   147601 tttgtaattt cgttctatct ggaaaatcta gcattttatc aactatttct tgaatagtca
   147661 tctgacttat tttttcacca catgtagggc aatacacatc tcctactctt gcaaataata
   147721 gtctcaaata atcatatatt tctgttactg ttccaaccgt agaacgtggg ttttttgagg
   147781 tagtcttctg gtcaattgag atagctggtg ataaaccctc tatatattct acatttggct
   147841 tttccatctg acctaaaaat tgtcttgcat aagcagatag actttctaca tatcttctct
   147901 gcccttctgc atatattgta tcaaatgcca aagaagactt cccagaccct gacaaacctg
   147961 taaaaacaat aaatttatct cttggtaatt ctaaatctat attttttaaa ttatgttctt
   148021 ttgctcctct tattataatt ttatcttcca taatgttgtt tttacctttc tatttttcca
   148081 atttttcttc taattgtttc actttatctc taagttctgc tgctctttca aattgtaagt
   148141 tttgagcagc ttccatcatc tctgatttta atttatctat attagctctt atttcgtctt
   148201 tatcatctgt atcagatatt ccatatacaa cttcttcctc tgcaacctta gtagcttcta
   148261 tactatctct tatatttttc tgtatagttt taggaattat attgtgctct tcattatata
   148321 aattttgtat atctcttctt ctctttgttt catcaatagc attttgcatt gagtcagtta
   148381 tcctgtctgc atacataatt actcttccat tttcatttct agctgcacgt ccaatagtct
   148441 gtattaaagc tgtttcggaa cgtaagaaac cttctttgtc tgcatctaaa attgctataa
   148501 gtgaaacttc tggtatatcc aatccttctc taagtaagtt tattcctaca aggacatcaa
   148561 acttccctaa tcttaaatct cttattattt ctgttctttc taatgtaact atatctgagt
   148621 gtaaatattt aacttttatc cctatttctt taagatagtt agtcaagtcc tcactcattt
   148681 ttttagttaa agtggttata agcactcttt catttttttc tatagtttta gttatttctc
   148741 ctaccaaatc atctatttga ttatttatag gtcttacttc tacaattggg tctaaaaggc
   148801 cagttggtct tattatctgt tctgctattg tctctgagtg ttgaatttca taaggtcctg
   148861 gtgtagcact tacaaacaac atttgattta tatttccttc aaattcttga aaatttaatg
   148921 gtctattgtc aagtgcagaa ggtaatctaa aaccattctc tataagtgat gtttttctag
   148981 acctgtctcc tgcatacata cctcttactt gtggtatagt gacatgtgcc tcatctacaa
   149041 ttatcaaata atcatctggg aagaaatcca ttaaagtata tggtctttct ccttctgacc
   149101 ttcctgtaat atgtctagaa tagttctcta tcccctgaca aaatcctatt tcctttaaca
   149161 tttctatatc atacttagtt ctttgttcaa ttctttgtgc ttctaatagc ctatcatttt
   149221 ccttgaaaaa tttaatttgt tcttgaagct cgttttctat ttctacaatt gccttttcaa
   149281 ttctttctgg cgttgttaca tagtgagatg ctgggaatat aacaacatga tttctagtac
   149341 caacaatctt ccctgttaca taatcaattt ctgttattct atctacctca tcaccaaaaa
   149401 attctattct tattgctttt tcatcattac tagctgggaa tatttctagt atatctcctc
   149461 taactctaaa ggtacctcgt gtaaagttta tgtcatttct ttcgtattgt atctctatta
   149521 acctttttat tacatcatct ctgtctcttt gcatacctgg tcttatagat aacataagtt
   149581 ctttatagtc ttttgggtca ccaagtccat atatacaaga tacagatgat attattatag
   149641 tatctcttct ctctaaaata gatgctgtag cagaatgtcg aagtttatct atttcatcat
   149701 ttatgcttgc atctttttct atataagtgt cactgtgagc tacatatgct tctggttgat
   149761 aataatcata gtaacttaca aagtattcaa ctgcattgtc tgggaaaaat tctttaaact
   149821 cactgtaaag ttgtgctgct agagttttat tatgagccat tattagggta ggtttcttaa
   149881 cttgttgaat gatatttgcc attgtgaatg tttttcctga acccgttaca cctaataatg
   149941 tcgaaaattt ctcatttcta tttatagaat ccactattga tttaatagct tctggttggt
   150001 cacctgtagg tttgaagtct gattttattt taaaatccat agtatcactc cttttttcga
   150061 acattagttc ctgacatgta ttattatatc atacctttat aaaattgttt aagttatttt
   150121 aaatatttta cccatttaaa ttttacttaa atctaaaaca taaaaaaaga cttctatctt
   150181 tgcaatagaa gtctataatt catggaaaat aaatacttta ttcaacttac ataataaaat
   150241 caaatgcttc tttttcaact ccatctaatt ttaaatcaaa catttttaat atagttgctg
   150301 catgtgctct caggtctcct tcttctataa ccacaccacc tttaatattt ggtccaaaag
   150361 ctataaatgg aggtctatta cctttagtag gtaaatgacc atgtgtcgca acagcaaact
   150421 tgtaatcttt tatatctgaa tcttttataa tacttccttc gaaatcattt ccaaatgaaa
   150481 ttccttcaag accttcaatc accatttcaa aatcaccttc aagaccaagg ttagatgctt
   150541 cctctttggt aaaaatattc tctatgaata attcttcttt aaattcttca agcagttctc
   150601 ttaattctgt taacctatct aagttattta ctattatttg agctgatatt ccagaagaat
   150661 ttacataggc atcaaaatcc ttaacttctc cattttcaac ttttataaat ccatttgatt
   150721 taagcaagac atttggattt ataactttat caactctaag atgaccatgg tccccaagta
   150781 ttataaaatt tgtatctttg tatgtacctg catcctttgt agcctgaata atatctccta
   150841 gccatttatc attcattttt aatgctttat ctacttcgtc attaaataag ccataattat
   150901 gtcgtgtatg gtccaatgta gctaaatgta ttagcattaa atctggttta tattccttta
   150961 ttatatctac tgaacagttt actccaaata aatccatgtt gggttctaat ttccaatcaa
   151021 tataatgaca atgtctattg taaataccgt ccattataac cttactggag cttcgttcaa
   151081 aagtttctct tgggtcatct tctctactag gagcccatat ttctgcaata ttataatcta
   151141 tatttggatt tgctcccatt acaggccata atacactaga ggtagttaga ttattttctt
   151201 ttgctacatc tataatagtt tttactttta tgtcctttga ataccagtac cagtctttat
   151261 tttctttatt tgggtcaaat ttttcattat gatatatacc atgtttttta ggatatactc
   151321 ctgtaaccat actagtatga cacggatatg tcagagtggg ataaacacaa tttatatttt
   151381 ttactatgga gcaattttct agtatagatt taaagtttgg tagatttttt acaaattcca
   151441 agtctttttc aaataaagaa tctactgata atactataac ctttgtcatt tacattacct
   151501 cttcatttat atattattga atacttttaa tcaacttaca agtctatata attatttatt
   151561 tgttttatct attactgctt gcattgcaga agttcctttg cttactacat cttttggatt
   151621 tgcatttaaa tcagtaagcc cctttttaat ctcatccatt agcttatcag ttgcttcctt
   151681 gtatccattt aacattggaa gtgcaacagc atattgtaat tggtcagttg caaccttata
   151741 ttgaggtttt tctttatata atgtttgtaa cttttctgag tttgttgcat cttcagttgt
   151801 tggtaagtat cctgttgatg aactaaactg aacgatttta tctgtttgtg tcatccattc
   151861 tataaattct gcacttgctt ctttttcttt atctgttcca ccttcaagca taactagatt
   151921 agctccacct gtaggagttg cgtatttttg attttttggt aggaatgctg ttgatatttc
   151981 aaacttacct tctgtttgtt gcataagtcc tgctaaatct gctgttgatg taaatatcat
   152041 acctacttta ccatttacaa aatcttgttt tgctacgtcc caggcattat aatcttctcc
   152101 aggaggcatt ttcattattc cagctttcat catatcttgc cagaatttaa ctggtgcttg
   152161 tcctgcctca ttattaaatg ctactttttt accattagtt atttctcctc cactttgcat
   152221 taccatagct tcatagaacc aaatatctat tggtgtttca aaaccataag tatcttctga
   152281 tttatttgtc attttactag cgtaatcctt aagctcttcc catgattttg gacctgaagg
   152341 gtcaagacct gctttagttg ccatatctct atttatgtat aatattggtg tacttctatt
   152401 aaatggcata ccaacaaact tatcctcata atatgaattt gttaaaagac cagaaaagaa
   152461 tctttcttga tatgttttat caaaatattt actcatatct actaaagcac ctgctgatgc
   152521 gaatgtacct gtttgagtta tttctaataa tgttacatct ggttggtctt tagatgttaa
   152581 ggctgcctgt agttttgttg cattttcata gtaatctttc tgttttaaca tatttacttg
   152641 tacatcctta tgttcactat taaattggtc aaccattttt tgagcagttt ctccattagt
   152701 tccacctaaa ggagcccaga aatctatctt cgtaatattg ctatcagtct tttctccttt
   152761 agatgcattt gcatcattag aatttgaaca cccaactaaa gaacctaata ataatgttat
   152821 tgctaatcct gttgctaatt ttcttttaag cttcattttc atcctccaaa tttagtcttt
   152881 aattttttat tttattcctg aatacataaa agcttctttt acttttttat tcgcaaatat
   152941 ataaacaaga attattggta aaattagaat tatatttcct gccattatta cattccatgc
   153001 agtaattcct tctgaagatt taaggagcgc aactcctatt ggtaaagttc ttattgcatc
   153061 tgtatttgtc attactaagg gccagaaata attattccaa tggtatataa aattaaataa
   153121 tataaatgtt attactgctg gttttgccat aggaaccatt attctaaaca ttatttttaa
   153181 gtcagaagca ttatctagtt tagctgcttc aatagtttca tctgaaatct gcataaaatt
   153241 ctgtctaagc agaaatatcc caaatgaaga tgtcataaat ggaattataa gtggaatata
   153301 agtatttata aatccaaact tactcatcat catatagact ggtagaaaag ttacttgtgg
   153361 tggaatcata agtcctacaa gcaaaagccc aaataacatt ttttcacctt taaatttaat
   153421 cttagaaaat gcatatgctg ctggaaccat aactaaaaat tgtaaaatta aaacacttcc
   153481 tgttacaaat acactgttta gtaaatatct aaaaaatgga cctgaatgcc atgctgtact
   153541 ataattttct aacactatat catttggtat aatagtaggt ggtaattgca tcatttcttg
   153601 tggattttta aaggatgtcg atagcatcca tataaaaggg aatataaata ttgcagataa
   153661 aagtaataga attaatattt ccattgattt tgatgctatt ttttttatat caactttgtc
   153721 ttttttgata ggttgtataa tatccattgt ctcctccttt tggataaatc ttataatatc
   153781 tactttttct ttattgataa tgaactttct ttgataagag tctaaagtta gcatatgtag
   153841 caatcataac tatagctaac aaaaacatac tcgctgcact cgcttgccct atgtcatagt
   153901 atataaatcc agactcatat atataataag ataacatgtt agttgaaccc actggcccac
   153961 cttgtgtcat tatacttatc tcatcaaata cttgaaaaga tgcagttgtt gttgttatca
   154021 taagaaaaaa tattgttggt gataatagcg gcaatgtcac tttaaagaaa ttcgttattt
   154081 tgtttgcatt atctaattta gatgcttcat atatatagtt tggtatggat ttaagacctg
   154141 ataatactat taagatattg tatccaagag tcttccatat tcctattaga attagtgaca
   154201 ttaaagctga actttcactt gaaagccact ttgatgttgg taaatgtaat gtcgatagta
   154261 tccagtttaa aaagccatat tgtggctcca taatccaaag ccacaataca gctacagata
   154321 ctagtgaaat tacatgtggt gtaaagatta tactttgcgt taaattgtgt attatagaat
   154381 ttttatttag ccaaacagca agtactatgg ctagtgatat agatatagtc accataaaaa
   154441 aagtataaat tgcagtgttc gttatagttt gtataaactc tggattcttt aaaagttctg
   154501 tataattgtt taagcctaca ttttcaaagg ttgaactaag aagactacca gatttgaagc
   154561 ttagagatat cgtcttaatt attggataga ctataaacaa taaagttcct ataatagcag
   154621 gtgttatata taaatatgct ttattaaatt gctttttcat aaatatctcc tttaatttta
   154681 gaatacatac ttttacatac ttttacatct tctattcttt ctccattttt atcaaaataa
   154741 tataaatctt tttctaatac acttattctt aaagttcttc caatatcaaa tatatcattt
   154801 tccgttttga ctcttatttc tctattaaga acagataaac aatatatagt ttcatttccc
   154861 aacacttctt tagtcacaac ttttccaaat aggctaaata catcatcatt ttttccatct
   154921 atgtttatac tctctagtgt tgctttttct ggtctgaatc caaatttaat atcatttttt
   154981 atatctagta tgttcataca aggactccct ataaattgag ctacaaataa attcttagga
   155041 ttttgatata tcttctttgg agaacctatt tgcattatct ttcctttgtt cattataact
   155101 atcttatctg ccattgacat tgcctcaacc tggtcatgag ttacataaac aaatgtagat
   155161 tttagttttt gatgtaattg tattaattct gttctcattt gatttcttaa tttagcatct
   155221 aaatttgaca atggttcatc cattaaaaat acctttggat ttttaaccat agctctagca
   155281 agtgccactc tttgtctttg acctccagat aagttgcctg gtttcacatt taaatattct
   155341 ctcaatccta ctacatcaat aacttctgtt ataagttttt ctcttttttc tttttctact
   155401 tttttgtttt taagaccaaa ttctatgttt tctctaacag tcatatgtgg atataatgca
   155461 taattttgaa ataccatagc tatatccctt ttactagcat ccgtttttgt aacgtcattt
   155521 tcttcaattt ttaacttgcc tgaagttata tcttcaagac ctgcaatcat tcttaatgta
   155581 gtagatttac cacatccact tggacctacc aatactgtaa aagaaccatc ttctatagta
   155641 agatttaaat tttctataac tgtatttttt ttattataag attttgttac attttctagt
   155701 cttatatctg ccatttttat cctcccaaaa tatttaattt aattctacga tttacttata
   155761 gattaataat actcttaatt tgtaaaggat atattaagac aaagtgaata tatagtaaac
   155821 ttattaaaat aaaggttaaa ttataaacat tttttgttaa atatactgaa gttaagatga
   155881 tatacagctt atgttagcta taattttcta tatttttcat attcatggtt tgctatcaca
   155941 atgtgatttt aatttatgaa gagtcatata aaactattta ttttttatat aatttggttt
   156001 gaaaataaaa aagacctcta tgggggatag aggtctttaa gtgggggaga ttgagtaatt
   156061 tatttttgct atattaagtt ttacaattat attatgtccc aaatttacaa ggtttaaaca
   156121 taaaaaataa aactttttta atttttataa atttacccaa aaaaaattac gtggctacgt
   156181 cctactctcc caggcagttt cccaccaagt accatcagcg ctagagagct taacttctgt
   156241 gttcggaatg ggaacaggtg tatcctctct gctattgtaa ccacataatc tttatgctta
   156301 atatttctat taagttttta ataagtttat agaacctagt aatactagtt tctttagagt
   156361 ta
//
LOCUS       DAMKPG010000002        96636 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMKPG010000002 DAMKPG010000000
VERSION     DAMKPG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 96636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.002"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            110..301
                     /locus_tag="PC621_000134"
     CDS             110..301
                     /locus_tag="PC621_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF4107325.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            456..1865
                     /locus_tag="PC621_000135"
     CDS             456..1865
                     /locus_tag="PC621_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF4107326.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1996..2685
                     /locus_tag="PC621_000136"
     CDS             1996..2685
                     /locus_tag="PC621_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF4107327.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2688..3623
                     /locus_tag="PC621_000137"
     CDS             2688..3623
                     /locus_tag="PC621_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF4107328.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3620..4513
                     /locus_tag="PC621_000138"
     CDS             3620..4513
                     /locus_tag="PC621_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF4107329.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4591..5337
                     /locus_tag="PC621_000139"
     CDS             4591..5337
                     /locus_tag="PC621_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF4107330.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5338..6171
                     /gene="rsmI"
                     /locus_tag="PC621_000140"
     CDS             5338..6171
                     /gene="rsmI"
                     /locus_tag="PC621_000140"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF4107331.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6194..6898
                     /locus_tag="PC621_000141"
     CDS             6194..6898
                     /locus_tag="PC621_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF4107332.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7086..7514)
                     /locus_tag="PC621_000142"
     CDS             complement(7086..7514)
                     /locus_tag="PC621_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF4107333.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7551..8981)
                     /locus_tag="PC621_000143"
     CDS             complement(7551..8981)
                     /locus_tag="PC621_000143"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF4107334.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9113..9697
                     /locus_tag="PC621_000144"
     CDS             9113..9697
                     /locus_tag="PC621_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF4107335.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9713..10234
                     /locus_tag="PC621_000145"
     CDS             9713..10234
                     /locus_tag="PC621_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF4107336.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10300..10546
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10630..12567
                     /gene="metG"
                     /locus_tag="PC621_000146"
     CDS             10630..12567
                     /gene="metG"
                     /locus_tag="PC621_000146"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF4107337.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12597..13367
                     /locus_tag="PC621_000147"
     CDS             12597..13367
                     /locus_tag="PC621_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF4107338.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13704..14135
                     /gene="phnG"
                     /locus_tag="PC621_000148"
     CDS             13704..14135
                     /gene="phnG"
                     /locus_tag="PC621_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF4107339.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14150..14725
                     /gene="phnH"
                     /locus_tag="PC621_000149"
     CDS             14150..14725
                     /gene="phnH"
                     /locus_tag="PC621_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF4107340.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14728..15813
                     /locus_tag="PC621_000150"
     CDS             14728..15813
                     /locus_tag="PC621_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF4107341.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15833..16702
                     /locus_tag="PC621_000151"
     CDS             15833..16702
                     /locus_tag="PC621_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF4107342.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16695..17561
                     /locus_tag="PC621_000152"
     CDS             16695..17561
                     /locus_tag="PC621_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107343.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17602..18315
                     /locus_tag="PC621_000153"
     CDS             17602..18315
                     /locus_tag="PC621_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4107344.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            18316..19485
                     /locus_tag="PC621_000154"
     CDS             18316..19485
                     /locus_tag="PC621_000154"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF4107345.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19489..20325
                     /locus_tag="PC621_000155"
     CDS             19489..20325
                     /locus_tag="PC621_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF4107346.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20326..21321
                     /locus_tag="PC621_000156"
     CDS             20326..21321
                     /locus_tag="PC621_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4107347.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21339..23027
                     /locus_tag="PC621_000157"
     CDS             21339..23027
                     /locus_tag="PC621_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF4107348.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23039..24106
                     /locus_tag="PC621_000158"
     CDS             23039..24106
                     /locus_tag="PC621_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4107349.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24592..24708
                     /locus_tag="PC621_000159"
     CDS             24592..24708
                     /locus_tag="PC621_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HDF4107350.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            24872..25939
                     /locus_tag="PC621_000160"
     CDS             24872..25939
                     /locus_tag="PC621_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4107351.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            26131..26661
                     /gene="rnmV"
                     /locus_tag="PC621_000161"
     CDS             26131..26661
                     /gene="rnmV"
                     /locus_tag="PC621_000161"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF4107352.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            26832..27701
                     /gene="rsmA"
                     /locus_tag="PC621_000162"
     CDS             26832..27701
                     /gene="rsmA"
                     /locus_tag="PC621_000162"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF4107353.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            27767..29038
                     /locus_tag="PC621_000163"
     CDS             27767..29038
                     /locus_tag="PC621_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107354.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            29189..30304
                     /locus_tag="PC621_000164"
     CDS             29189..30304
                     /locus_tag="PC621_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF4107355.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            30490..31386
                     /locus_tag="PC621_000165"
     CDS             30490..31386
                     /locus_tag="PC621_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF4107356.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            31456..32253
                     /locus_tag="PC621_000166"
     CDS             31456..32253
                     /locus_tag="PC621_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107357.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(32309..33661)
                     /locus_tag="PC621_000167"
     CDS             complement(32309..33661)
                     /locus_tag="PC621_000167"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF4107358.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            33880..34758
                     /gene="purR"
                     /locus_tag="PC621_000168"
     CDS             33880..34758
                     /gene="purR"
                     /locus_tag="PC621_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF4107359.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            34914..35192
                     /gene="spoVG"
                     /locus_tag="PC621_000169"
     CDS             34914..35192
                     /gene="spoVG"
                     /locus_tag="PC621_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF4107360.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            35402..36781
                     /gene="glmU"
                     /locus_tag="PC621_000170"
     CDS             35402..36781
                     /gene="glmU"
                     /locus_tag="PC621_000170"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF4107361.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            36876..37826
                     /locus_tag="PC621_000171"
     CDS             36876..37826
                     /locus_tag="PC621_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF4107362.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      38666..38751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            38913..39434
                     /locus_tag="PC621_000172"
     CDS             38913..39434
                     /locus_tag="PC621_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF4107363.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     gene            39585..41261
                     /locus_tag="PC621_000173"
     CDS             39585..41261
                     /locus_tag="PC621_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF4107364.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            41273..42481
                     /locus_tag="PC621_000174"
     CDS             41273..42481
                     /locus_tag="PC621_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF4107365.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            42496..44208
                     /locus_tag="PC621_000175"
     CDS             42496..44208
                     /locus_tag="PC621_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF4107366.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            44201..45109
                     /gene="pilO"
                     /locus_tag="PC621_000176"
     CDS             44201..45109
                     /gene="pilO"
                     /locus_tag="PC621_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF4107367.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            45136..45702
                     /locus_tag="PC621_000177"
     CDS             45136..45702
                     /locus_tag="PC621_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF4107368.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            45739..46266
                     /locus_tag="PC621_000178"
     CDS             45739..46266
                     /locus_tag="PC621_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF4107369.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            46287..47825
                     /locus_tag="PC621_000179"
     CDS             46287..47825
                     /locus_tag="PC621_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107370.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            47925..49007
                     /locus_tag="PC621_000180"
     CDS             47925..49007
                     /locus_tag="PC621_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF4107371.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            48988..49770
                     /locus_tag="PC621_000181"
     CDS             48988..49770
                     /locus_tag="PC621_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF4107372.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            49872..50522
                     /locus_tag="PC621_000182"
     CDS             49872..50522
                     /locus_tag="PC621_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF4107373.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            50536..51096
                     /locus_tag="PC621_000183"
     CDS             50536..51096
                     /locus_tag="PC621_000183"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF4107374.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            51113..54499
                     /gene="mfd"
                     /locus_tag="PC621_000184"
     CDS             51113..54499
                     /gene="mfd"
                     /locus_tag="PC621_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF4107375.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            54530..55525
                     /locus_tag="PC621_000185"
     CDS             54530..55525
                     /locus_tag="PC621_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF4107376.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            55649..56188
                     /gene="spoVT"
                     /locus_tag="PC621_000186"
     CDS             55649..56188
                     /gene="spoVT"
                     /locus_tag="PC621_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF4107377.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            56308..57921
                     /locus_tag="PC621_000187"
     CDS             56308..57921
                     /locus_tag="PC621_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF4107378.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            58028..59476
                     /gene="mazG"
                     /locus_tag="PC621_000188"
     CDS             58028..59476
                     /gene="mazG"
                     /locus_tag="PC621_000188"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF4107379.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            59565..59843
                     /locus_tag="PC621_000189"
     CDS             59565..59843
                     /locus_tag="PC621_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF4107380.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            59915..60157
                     /locus_tag="PC621_000190"
     CDS             59915..60157
                     /locus_tag="PC621_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF4107381.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            60219..60473
                     /locus_tag="PC621_000191"
     CDS             60219..60473
                     /locus_tag="PC621_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF4107382.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            60474..61007
                     /locus_tag="PC621_000192"
     CDS             60474..61007
                     /locus_tag="PC621_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107383.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            61013..61297
                     /locus_tag="PC621_000193"
     CDS             61013..61297
                     /locus_tag="PC621_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF4107384.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            61410..62330
                     /locus_tag="PC621_000194"
     CDS             61410..62330
                     /locus_tag="PC621_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF4107385.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            62420..64792
                     /gene="spoIIE"
                     /locus_tag="PC621_000195"
     CDS             62420..64792
                     /gene="spoIIE"
                     /locus_tag="PC621_000195"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF4107386.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            65143..67035
                     /gene="pepF"
                     /locus_tag="PC621_000196"
     CDS             65143..67035
                     /gene="pepF"
                     /locus_tag="PC621_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF4107387.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            67149..68114
                     /gene="galU"
                     /locus_tag="PC621_000197"
     CDS             67149..68114
                     /gene="galU"
                     /locus_tag="PC621_000197"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF4107388.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            68614..70218
                     /gene="rho"
                     /locus_tag="PC621_000198"
     CDS             68614..70218
                     /gene="rho"
                     /locus_tag="PC621_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF4107389.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            70371..70571
                     /gene="rpmE"
                     /locus_tag="PC621_000199"
     CDS             70371..70571
                     /gene="rpmE"
                     /locus_tag="PC621_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF4107390.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            70716..71609
                     /locus_tag="PC621_000200"
     CDS             70716..71609
                     /locus_tag="PC621_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF4107391.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            71627..72475
                     /gene="prmC"
                     /locus_tag="PC621_000201"
     CDS             71627..72475
                     /gene="prmC"
                     /locus_tag="PC621_000201"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF4107392.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            72519..73583
                     /gene="prfA"
                     /locus_tag="PC621_000202"
     CDS             72519..73583
                     /gene="prfA"
                     /locus_tag="PC621_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF4107393.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            73712..74392
                     /locus_tag="PC621_000203"
     CDS             73712..74392
                     /locus_tag="PC621_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF4107394.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            74421..75461
                     /locus_tag="PC621_000204"
     CDS             74421..75461
                     /locus_tag="PC621_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF4107395.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            75478..75927
                     /locus_tag="PC621_000205"
     CDS             75478..75927
                     /locus_tag="PC621_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF4107396.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            75944..76396
                     /gene="rpiB"
                     /locus_tag="PC621_000206"
     CDS             75944..76396
                     /gene="rpiB"
                     /locus_tag="PC621_000206"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF4107397.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            76432..77061
                     /gene="upp"
                     /locus_tag="PC621_000207"
     CDS             76432..77061
                     /gene="upp"
                     /locus_tag="PC621_000207"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF4107398.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            77361..78176
                     /locus_tag="PC621_000208"
     CDS             77361..78176
                     /locus_tag="PC621_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF4107399.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            78402..78839
                     /locus_tag="PC621_000209"
     CDS             78402..78839
                     /locus_tag="PC621_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF4107400.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            79024..79251
                     /locus_tag="PC621_000210"
     CDS             79024..79251
                     /locus_tag="PC621_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF4107401.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            79256..79642
                     /locus_tag="PC621_000211"
     CDS             79256..79642
                     /locus_tag="PC621_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF4107402.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            79645..80349
                     /gene="atpB"
                     /locus_tag="PC621_000212"
     CDS             79645..80349
                     /gene="atpB"
                     /locus_tag="PC621_000212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF4107403.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            80418..80678
                     /gene="atpE"
                     /locus_tag="PC621_000213"
     CDS             80418..80678
                     /gene="atpE"
                     /locus_tag="PC621_000213"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF4107404.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            80801..81298
                     /gene="atpF"
                     /locus_tag="PC621_000214"
     CDS             80801..81298
                     /gene="atpF"
                     /locus_tag="PC621_000214"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF4107405.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            81295..81840
                     /locus_tag="PC621_000215"
     CDS             81295..81840
                     /locus_tag="PC621_000215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF4107406.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            81857..83359
                     /locus_tag="PC621_000216"
     CDS             81857..83359
                     /locus_tag="PC621_000216"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF4107407.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            83387..84265
                     /gene="atpG"
                     /locus_tag="PC621_000217"
     CDS             83387..84265
                     /gene="atpG"
                     /locus_tag="PC621_000217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF4107408.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            84278..85672
                     /gene="atpD"
                     /locus_tag="PC621_000218"
     CDS             84278..85672
                     /gene="atpD"
                     /locus_tag="PC621_000218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF4107409.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            85675..85935
                     /gene="atpC"
                     /locus_tag="PC621_000219"
     CDS             85675..85935
                     /gene="atpC"
                     /locus_tag="PC621_000219"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF4107410.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            86397..86777
                     /locus_tag="PC621_000220"
     CDS             86397..86777
                     /locus_tag="PC621_000220"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF4107411.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            86792..87253
                     /locus_tag="PC621_000221"
     CDS             86792..87253
                     /locus_tag="PC621_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF4107412.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            87274..87864
                     /locus_tag="PC621_000222"
     CDS             87274..87864
                     /locus_tag="PC621_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107413.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            87879..89036
                     /gene="alr"
                     /locus_tag="PC621_000223"
     CDS             87879..89036
                     /gene="alr"
                     /locus_tag="PC621_000223"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF4107414.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            89069..89356
                     /locus_tag="PC621_000224"
     CDS             89069..89356
                     /locus_tag="PC621_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF4107415.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            89325..89708
                     /locus_tag="PC621_000225"
     CDS             89325..89708
                     /locus_tag="PC621_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF4107416.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            89879..90706
                     /locus_tag="PC621_000226"
     CDS             89879..90706
                     /locus_tag="PC621_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF4107417.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            90699..91640
                     /locus_tag="PC621_000227"
     CDS             90699..91640
                     /locus_tag="PC621_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF4107418.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            92063..93157
                     /locus_tag="PC621_000228"
     CDS             92063..93157
                     /locus_tag="PC621_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107419.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            93383..93799
                     /locus_tag="PC621_000229"
     CDS             93383..93799
                     /locus_tag="PC621_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107420.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(93981..94550)
                     /locus_tag="PC621_000230"
     CDS             complement(93981..94550)
                     /locus_tag="PC621_000230"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF4107421.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            94704..95861
                     /locus_tag="PC621_000231"
     CDS             94704..95861
                     /locus_tag="PC621_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF4107422.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            96048..>96636
                     /locus_tag="PC621_000232"
     CDS             96048..>96636
                     /locus_tag="PC621_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4107423.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFGEITLNIPRDRNGDFEPKVIRNYENDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLIAKITDRVLPLAHEWQSRALDM
                     VYPIVFMGAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT        38816 a        10392 c        17782 g        29646 t
ORIGIN      
        1 attttttgtc aaaaaaaaca aaaaatacgt atttattttt tcatttcggg aaaactaaac
       61 acaggaatta tggactttat aaaaatttaa ttcttgagga gtgatataag tgaaaaaagt
      121 tgctttaatt ttagctataa taggtgcgct taactggggt gcaattggta ttctaggtac
      181 agacttaata ggtagtatct ttggtggaac atatgaaatg gtaagtagaa taatatactt
      241 cattgtaggt ttagctggat tatacttaat accaagttta atgtctgatg atagagaata
      301 atttatgctt ttatgttaaa tacctcattc taaatgttta gaatgaggta tttaatttta
      361 taaaaatatt aatttgtttc ttttttaatg gtaaaataga taagaattga agttttaaaa
      421 agaagtatta tgtaaaaaag gagtactatt aatatatgtt tattattaat gaattaaata
      481 agattgtaaa tgaagaagta atatcttttc atatgcctgg tcataaaaag ggacaggttt
      541 ataaaaagtt aggatataaa gatatactaa aaaatttgta taaaatggat acaactgaga
      601 tattaggaac agataattta cactttccgg aaggaattat aaaaaattca caaaaaaaag
      661 catcaagagt atttaaaagt gattatactt gctatttagt aaatggtagt agctgtggaa
      721 tacaatctgc aataatgtca gtatgtagtc ctaaaagtaa aataataaca aatagagact
      781 gtcatcagtc tgttataaat ggatgtattt taggagatat agatatagag tatgtgcctt
      841 gtgagataag tgaagagaca aatgtcttaa aagggatatc tataagtaat gtgattgata
      901 taatagataa aaacttggat gcaaaagcag tactgcttac ttatcctaca tattatggaa
      961 tgacatatga tttagaatac atatgtaatt atgcacatag caaaaagatg gtagtgatag
     1021 tagatgaagc acatggtgcg catttagggc ttagtgagag gcttcctaaa acagctcttg
     1081 agcaaggtgc agatatagtt gtacagagta cacataaaac tcttccagca tttacacaat
     1141 cgtcaatgat acatatacag ggagaccgag ttgatataga taaaatattg aacatgttaa
     1201 gaattacaga atcttcaagc ccttcatatt tattattatc ttctctggaa ttagcagtag
     1261 atatatatga aaataaagga aaagaattaa tggaagagct tttaaataat attagaattt
     1321 tcaaagataa agttgattca aatattaata tatatgacac aaatgataaa acaaaaatat
     1381 ttatatcatc taaaaacatg ggtttaacag gttatgaact agaaaatatt ttgagaaaca
     1441 aatataacat acaagtggag ttatctaatt attatggagt tcttttaata tgtacaatag
     1501 gaaatactgc tgaggatttc tctagtttag aaacagcatt aaacaatgtt gtaaaggaag
     1561 actttaaatt tgaaaagtta gaaaataaaa aataccctgt tgttattcca aagaaaatat
     1621 taacacctag agaagcattt tataaaacta aaaaaagtgt taaaatatat gacagcatag
     1681 gtaaaatttg tggtgaatgt atagttccat acccacctgg aataagtgta atatctcctg
     1741 gtgaagtaat aagtaaagaa ataatagact atctaatgtt ttgtcattct aaaggcatga
     1801 ttgtgagtgg attgaaagat gtagatttaa attttataca agtaatagat ttagaagaca
     1861 tttaaaaatt atgatataaa agtagataga agtagatagt ttaaaattgt gaagagaaat
     1921 aaatagactg cttatataga gctatctaat aaaagtacta aaaaagtaaa aagtgtttgt
     1981 atggaggata tgaaaatgaa aggtaagtta ataattatag agagtggttc agatgcaagt
     2041 ggaaaggcta ctcagacaga aatattatat gaaagattaa aaaaagaagg ctatatgatt
     2101 aaaaaggtag aatatccaaa ttatgaatct ccatcatctg ccttagtgaa aatgtaccta
     2161 aaaggagact ttggtaaaaa tgcatcagat gtagacccat atatagcatc aacttttttt
     2221 acagctgata ggtatgcatc atttaaaacc gattgggagg agttttacaa tgaaggtgga
     2281 ataattatcg cagatagata tacaacctct aatatggtac atcaggcctc taaaatggat
     2341 atttgtgata gagaagagta tttaaattgg ttagctgatt atgaatttaa tttatttaag
     2401 ataccacaac ctgattgtgt tatattttta gatgtgccta ttgaatttag taaaaagctt
     2461 atggagaata gaaaaaataa aattacaggg gaagaaaaaa aggatattca tgaaagtgac
     2521 atagagtatt tagagaaatc ttacaacaat gctttatata tagcagataa atatgattgg
     2581 aaaaaaataa attgtgtttt agataacaaa ttaagaagta ttgaaagtat acataatgaa
     2641 atttataaaa tagttcttga ttcaataaaa cctatggaga gataaatatg tattttgaaa
     2701 atattatagg gcaagatttt gcaaagaaat atttaacaaa ttctataaag aaaaataaac
     2761 ttaataatgc gtatatgttt gaaggaatgg atggaatagg aaaaaagaaa tttgcagatg
     2821 aattatcaaa actattactt gattatgaaa atctcgaaaa tagtccagac tatgtactca
     2881 taaaaccaga tggaaatagt ataaaaatag ctcaaatcag aaatttacaa tcagatatag
     2941 taataagacc tcataaagac tataaaatat atataatcaa taatgctgaa aaaatgactg
     3001 tggaagctca aaatgcactt ttaaagacgc tagaggaacc tccaaactat gctataataa
     3061 tcttagtcac aaacaacaag gaatctttat tagaaactat aaaatctaga tgtgatataa
     3121 taaagttctc acctatacca attgaagact taaaacgtta tttgataaat acaggtatag
     3181 aagaagaaag agctcaatta ttggctattt tttcgagagg aagtattgag aatgctctaa
     3241 atctatctca gtcttctgag ttttcaatga tgagagaaga tatacaacag tatattcaga
     3301 taatgttaga taaaaacata gtggagattt taaatatacc aaacaatatg gaaaaatata
     3361 gaggtaagat tatagcttta ttagatatga tgataaacta ttttcgagat atcatcctat
     3421 taaaagaaaa tgtaaacaag aacatgttaa taaatgtaga taagctagtt ttcattcaaa
     3481 atatgagtgg aaaaattagt tattctcaat tgtccaagat tattgatata atagaagatg
     3541 caaagagtaa aataaaaagc aattgcaatt tcaatataag catacaagtt atgtctttaa
     3601 atatatacga ggtgattaaa tgataaagat agtaggtata aggtttaaaa gtgctgggaa
     3661 aatatattat tttgaccctg ttgattttaa tatagagcaa gatatggatg tagttgtaga
     3721 aactgcaaga ggcttggagt atgggaaagt agtcgttggt ccaaaagata tggatgaaag
     3781 cgaattagtt tctcctttga agccaattat aagaatagct acagaagaag ataaaaaagt
     3841 atatctagaa aataaggaaa aggcaaaaga aacttttgag ttgtgccaac aaaaaataaa
     3901 agaacatgag ttaacaatgt ttttaataga ttgtgagtat acgtttgata ggaataagtt
     3961 gattttttac tttacagcag aaggaagaat agattttaga gaattggtaa aagacttagc
     4021 tgctatattt aaaactagaa tagaacttag acaaataggt gtcagagatg aagctaaatc
     4081 tataggaggt ctagggcctt gtggtagaaa actttgttgt tcatcttggt taggtgattt
     4141 tcaaccagtt tcaataaaaa tggcaaaaga ccaaagtcta tctctaaatc caacaaaaat
     4201 atctggaata tgtgggagat tattctgttg cttaaaatat gagcatgatg tatatagtga
     4261 agcaatagaa aaaatgcctg ttgtaggtgc attggtgcaa tcagatgatg gaaaaggaaa
     4321 agttgtagaa gtgaatcctt tattagaaca aattaaagtg gaatttcaag acaagacagt
     4381 aaagatatac acaagagaag atataaaaat acttagagag cctaaaaaat gtgatggctg
     4441 tggtggtaaa tgtaaggata atgatggatt agatgaagcc acactaagag agctaaagaa
     4501 attagaagat taaatttaag gtagggcttt tcctaccttt tttaaagcaa ttaatgctta
     4561 aagttattaa tatttgagga gaatttatat atgcaattaa aagattcaga aagaatagat
     4621 gatttacaat taaaaggcct taaaattata caagatacta atggtttttg ttttggtata
     4681 gatgcagttt tattagccaa ttttactaaa ataaaaaaag atgccaaagt agtagatttg
     4741 ggtactggaa ctggaattat acctattttg atagctggaa aaagtgaagc taaaaaaatt
     4801 attggcgtgg aaatacaaga agatgtatat gaaatggcaa ctcgttctat aaaattaaat
     4861 ggattagaag aaagagttga gattataaat gaagatataa aatacataga caaagtctta
     4921 gacataaatg gttaccatgt agtcacatca aatcctccat atatgcatat tgatggaata
     4981 aaaaatccaa atgataaaaa agcaatatca agacatgaag taaggtgcaa tttggaagat
     5041 gtaattagag cttcatccag attggttatg cctagaggaa aattcttcat gatacataga
     5101 ccaacaagat tggttgatat aataacttta gggagaaaat ataaattaga accaaaggtt
     5161 attcaatttg tgcatccaag acctcaaaaa gcaccgaact tagttttggt tcaatttgta
     5221 aaagatggaa gaccagagct taaaatattg gacccattgt atgtttatgg agaagatggg
     5281 aattatacaa aagaattaaa agcaatatat aataataaag atataggaga gttgtaaatg
     5341 agtgggaaat tgtacatatg tccaacacca ataggaaacc tagaagatat aacatataga
     5401 actttaagaa cattaaaaga agtagacttg attgcagcag aagatacaag acatagtata
     5461 aaacttttaa atcattttga aatctcaaaa ccactaacta gttatcatga acataataag
     5521 gattcaaagg gagattattt gataaataaa ctaattgatg gtgaaaatat agctctgata
     5581 agtgatgcag gtatgcctgg tatatctgac ccaggagaag agataataaa acaagctata
     5641 caaaataaca tagagataga agtattgcca ggtgcaacag cctttgtaac ggctttagtt
     5701 ggctctggta tggacacaca tagatttgtt tttgagggct ttttagatag agataaaaaa
     5761 gttaggaaag cacaattgga agaattaaag gaagaaagta gaactataat tttctatgaa
     5821 tcaccacata gattgaagga tacactaaaa gatatgctta aaattcttgg taataggaat
     5881 atttctataa atagagaact tacaaaaaaa tatcaagaaa taataagaga aaatatagaa
     5941 actaccataa aaatatttga ggaaaaagaa gtaagaggag agtttgtatt aatagtagag
     6001 ggttttcatg gtgagaagtc aaagaaagaa aattatgatg ggcttacaga tagggaatat
     6061 gttttaaaac taattgaaag tggtatgaac aaaaaagatg caataaaaat agtttgtaaa
     6121 gatagaaagt taaagaaaga tgtggtttat aaacaagttt tagatttata aaacctgaat
     6181 ctgaggtgat tttatggaaa tagggaaaat tataatagag agtttaaaga gggaagatac
     6241 acataaaata tttatagaat tagataataa gcatgaatta gaaaaaataa ttcctaaaat
     6301 aagctgtatg aagtctgtag gtgaatgtaa atatcatgta gtaaattgtt ttgaacattc
     6361 tataaatgca ttaaaagaat tagaaatagt attaaatgat aaggattttt ttccaataca
     6421 tctaagaaaa catgtgtcaa actatttaaa cacatatatt gaagaaggaa taaataagtt
     6481 acatgtatta aagttgggaa catttttaca tgatataggt aagcctgata gtatgacctt
     6541 agatgaaacc ggaagagttc atttcacaaa tcatgaaaaa ataggtgctc aaataattga
     6601 caatatggga ataaagttag agttatcaac agagacatat agacttataa gtaaatatgt
     6661 aagatatcat atgatacttt tgtctctata taagaaaaat gatttgagta ggaaagaatt
     6721 aattaatgta tttaacttag tagatgagga cactataggt gtaataatgc ttgggtatgc
     6781 agatatagtg tctacagtaa aattattggg caagagtgga gaagtcagtg tgttaaagac
     6841 atatatggaa tatattttaa caaattactt gtataagagt aattatacac atgtataaat
     6901 ttagttaata taattgagtt ttagatgatt aattgaagtt tgaaatatac ggatttcaaa
     6961 aacataaaag aagttgtctt tgttgtaaaa ttatgaaaga caactttttt tatatcaaaa
     7021 attgaaattt tattaaggat acttaatggt ctattaatta ataaaccatt agtatttaat
     7081 ttatattaca atattgattt tgcttgtatt ggcgttgata gtataacaga ggtcttagta
     7141 gaaccaattt tctttatagt atcaatcaca ttttctagct cgtacatatc ttttacaatt
     7201 acttttaaaa gtaagcaatc atcacctgtt atatggtgac attctacaat cctagggtcc
     7261 tttgcagctg actcaataaa ttctgtatat ccattgcttg gaagagaaat atgaataaat
     7321 gcctttataa ctctgcctaa tgaatctggg ttgacaatag ctttatatcc ttctataaca
     7381 ccagactctt ctaatctttt gactctttct gaaactgcag gagaagttaa accaactatt
     7441 tttcctaagt ccttcataga aattctccca tcatcctgta aaatttctat gattctgtaa
     7501 tctgtaacat ccataaataa acagtccccc ttaaataaaa ataattaata ctataaaact
     7561 tttttagaat ctatcatttg tgtttttaaa taccaaagat ttctagataa atcaacctta
     7621 taaatgtgtg gttttgaaag tcttttgtat tgttcccaga tagtacttaa ttcatattgt
     7681 acatgatttt ttatttcaag aacagctttt ttattatatt tgcatctgcc ttttatatac
     7741 aatggtttaa gaagctcttt aactttgtaa tttgtaaata cctttgtttt ccatgtataa
     7801 gtaggatgga agatttctaa aggtttagac tcatctataa cttcatcatg taacataatc
     7861 aaatctgcct ctgccttatt gtcttcgtta tatattctta taactttctt gtaaccagga
     7921 ttatttattt tttctggatt ctcagaaatc tttatcttag gttcaagaac tcctttttca
     7981 caagatgcag ctaatttata aactcctcca agagaaggtg aatcgaaaga tgtaatgagc
     8041 tttgtaccta ctccccatga atttatagta gctccttctg ctttaagtga tgttatggta
     8101 tattcgtcta ggtcattaga tgcagtgatg ctgatatctg aaaagccagc gttatctaat
     8161 tgttttttag cttctttaga taaataagct aaatctccag agtcaagtct tatacccata
     8221 ggtttgtatc ctttttcaga aatatttttg aatactttta ttgcattagg aactccactg
     8281 tttaatacat cataagtatc tacaagtagt aggcatttat ctggataaac atcagcatat
     8341 gcttggaatg cttcaagttc attatcaaat ttttgtaccc aactgtgagc ttgtgtacca
     8401 attataggta tatcaaacat tttccctgct agtacgtttg cagttccagc acaaccacca
     8461 actacagcag ccctagctcc atatatccct gcatctggac cttgtgcacg acgtaatcca
     8521 aattcaaata cagggtctcc ttgagctgca aagcaaactc tagaggcttt agtagcaata
     8581 agagactgaa aattaactat tgttaacaat gctgtttcaa taagttgagc ttgatataaa
     8641 ggtgccttaa cagtaattaa tggctcatta gggaacatga tagttccttc ctcaacagca
     8701 tatatatctc ctgtgaattt aaattctttt aaaaatttta aaaacttgtc agaaaataaa
     8761 tttaagtttt ttaagtactc taaatcttca tctgaaaaat gaagattatc tatgtattca
     8821 acaacctgat caataccaca aactatagta taccctccat cacaagcgtt ttttctaaaa
     8881 aacatatcaa atacaacaat gtcttcatga atattctttt caaaatagcc atttaacata
     8941 gtaagctgat ataaatcagt aagaagtgtt agatttctca ttatttctct ccttaattat
     9001 tattaaatat taaaaattgt aaaaactccc acacttatac taaaacataa tatagtacaa
     9061 ctttcaagta taaagtatta cctaactatc ataaaattat ttaaagagga ggatgattat
     9121 aataggaaaa atatggtttt atatggtatc gataggaata ataggaagct tgatttctaa
     9181 aaatatgggt gaattaaata aagttatatt aagtgaaaca tcaaaaggta tagagtttgc
     9241 aataagtcta gctggtatca tggcactctg gatggggata atgaatatcg caaaagaatc
     9301 aggtttaatt gaaaagatag gtcaaaaact aaatcctata atgagaagat tatttccttc
     9361 tattccaaag ggtcataaag caatgtctta tatagttatg aatattgctt taaatatgtt
     9421 gggtgctgga aatggtgcaa cagcatttgg tttaaaagca atgagtgagc ttcaaacttt
     9481 gaataacaag aaagatacag ccactaatga tatgattatg tttatggtaa tcaatatatc
     9541 atctatacaa ataattccat ttactataat caaattaagg atggatatgg gggctcaaaa
     9601 tccaagtgaa ataattttta cgactttatt tgcaaccata atatctacag cagtagcaat
     9661 aataacatgt aaattttttc aaagaagata tagataaagg ggatgtagat acatggagat
     9721 attttcaaac ttacttatac caataataat tctgtacata gtagtttatg gtaaatataa
     9781 aaaaatagat gtgtatgata gttttgtaaa aggagcaatt gatggattaa aagcagcatg
     9841 ggatatccta ccatatataa taggtatatt tttggcaata ggaatattca aaactggtaa
     9901 aggtcttgat atgttagaat ggctatttac accaatagca aatatgatga gtataccaaa
     9961 agaattaata ggtctaataa gtgtaaaacc tttatctgga agtggtgctt tagggatgta
    10021 cagtgaactt gcaaatagag ttggaatagg aagtttagta gaaaaaatgg gtgctacgat
    10081 tgtaggttct tctgagacta ttttttacac aatggcaata tactatggaa gtctgaagat
    10141 aaaaaatact agacatacat tatcatgtgc tatgattagc catgttgcag gtgtcatagc
    10201 agctgttttt atttgctatg taatatttgt ataatttgtt gacaatatgg gaaaaatttt
    10261 ataatatagt gattatatag aaacaaatag taatacaaat tacaagctat gaaaagaaga
    10321 gtaaataaag gttttttcta tcagagagct aggttaggtg aaagctagtg tcaaaagctt
    10381 tattgaagat ggtcttggag ctttttatct gagcaagtgt gctagggtaa aacggttgga
    10441 cacgttatag tctactaagg tatctataaa atgtttctac atttcatgtg aaacataact
    10501 attatagatg aattagggtg gtaccgtgaa taatctcgcc cctatgttaa ctcataggag
    10561 gcgatttttt attatttaaa tgaaagaatc atatgattac gaacgataaa aaattaggag
    10621 gtaaataaca tgagcaaacc gagtttttat gtaacaacac caatatacta tccaagtgga
    10681 ggattacaca taggacatac ttattcaaca gtagctgcag atacaatagc tagattcaag
    10741 cgtttctgtg gatatgatgt aaagttctta acaggaacag atgagcatgg tgaaaaaatt
    10801 caaaagaaag ccatagaaca aggaatgtca gagatagagt atcttgatgg aatgataaaa
    10861 gatataaagg ccttatggaa tactatggat atatcttatg atgattttat aagaactaca
    10921 gaaaaaagac atacagacat aatacaaaag atatttacta aactatatga gcaaggtgat
    10981 atatataaag gtgaatatga aggtagatac tgtactcctt gtgagagttt ctggacagaa
    11041 tctcaattac tagaaggaaa taaatgccct gactgtggaa gagaaacata tttagtaaaa
    11101 gaagaatctt atttctttag attatctaaa tatgaagaca gattaaaaga gttatttaaa
    11161 gatgatagtt tctgttttcc tgctgctaga aaaaatgaaa tggtggcaaa ctttttagac
    11221 aaaggattag aagatttaag cgtaactaga acgacttttg actggggtat aaaagttcct
    11281 tttgatgaaa aacatgtaat ttatgtgtgg gtagatgctt tatgtaacta tataacagca
    11341 ttaggttata tgactgataa tgatgaagaa tttaaaaaat attggcctgc caatgttcaa
    11401 atagtaggta aagaaatagt gagattccat acaataatat ggccagcgct tttaatggct
    11461 ttaggtctag aagttccaaa acaagtattt ggacatggat ggatactatt tgctgatgat
    11521 aaaatgagta aatcaaaagg taatgtggtt tatccagaac ctataataga aagatatgga
    11581 atagacacac ttaagtactt cttattaaga gagtttgcat ttgggcaaga tggaagttat
    11641 actcatagaa attttgtaac gagaataaat tatgatttag caaatgattt aggaaactta
    11701 ataagtagaa ctgtagctat ggttgaaaaa tacaataatg gtataatacc aacagcaaaa
    11761 gtttcaactg attttgatgc tgacttaaaa gaacaagctg tttcaactag agaaaacttt
    11821 gaagctgaaa tggataaaat gcaattccat gaagcattag agagtgtatg gaaattagtt
    11881 agaagaacta acaaatatat agatgaaact atgccttggg cactagctaa agatgaaacg
    11941 aagaaagatg aattagacac agttttatat aatttatgtg aatctataag aataatcgca
    12001 acactaataa atcctataat gaatgagact gctaataaaa tatatgaaca tataggaata
    12061 aaaggtcaag atgatataac tacatgggaa agtacaaaaa catttggtct tattggagaa
    12121 aatgtaaaag tatttaaagg tgaaccttta ttcccaagat tagatgtaga aaaagagata
    12181 gaagaattaa ctaaaatgtt ctctgggaaa cctccagtag aagaaaaacc tttagagcat
    12241 aaagaagaaa ttactattga tgatttagac aaaatagagc ttagagtagg gaaaataata
    12301 agctgtgaaa aacatccaaa agcaaataaa ttattagtat cacaagttaa gattggacca
    12361 gaaacaagac aaatagtatc aggaatagct gaatactata agccagagga tttagtagga
    12421 aaagaggtta cagtggtatg taacttaaaa ccagttaaat taagaggtgt agaatctcaa
    12481 ggtatgatat tagcagcagg tgatgatgga gacccatatg ttttaccatt tacacaaggg
    12541 gctaaagatg gatgcgaggt tcgttagtag gtaattaaat agtaaggaga ttaaagatgt
    12601 tatttgactc acatgcacat ttaaatgatg aaagttttga tgaagataga gatgaactta
    12661 ttggttcatt aaaagataaa ggtgtagatt tggttgtaaa tccaggagca gatatagaga
    12721 cgtcgataac agcaattgaa cttgcgaaaa aatacgactt tatatattca gcagttgggg
    12781 tacatccaca tgatgtatca aaactagatg atacagcaat agaaactcta agaaaacttg
    12841 caactgagaa tgaaaaagtt gtagctattg gagagattgg attagattat tattatgatt
    12901 attctccaag agaagaacaa aaagagtggt ttaagaagca aattgaatta gctaatgagc
    12961 taaaacttcc aataataata catgatagag atgcacatgg agatactttt gagataataa
    13021 agaatactaa aaatcctgaa ataggatgtg tacttcattg ttatagtgga aatgtagagt
    13081 tagctagaga atatgttaag atgggttgtt atatatcaat tccaggcacg gttactttta
    13141 agaataataa aaaaactaga gaagtagtaa gagaaatacc tctagaaaga ttattcatag
    13201 agactgattc accatatatg tcaccagagc cacatagagg aaagagaaac aatccttctc
    13261 aagtatcttt tgttgctgat aagatagccc aggaaaaagg gatatgttat gaagaagtat
    13321 gtagagttac aaaagaaaat gcaaagaaat tttttaatat aaaatagaca aattaaatct
    13381 tataatgata tatataataa gtcattataa gtataggatg agagtgcctc aaaatgagtt
    13441 ttttagttca tgaggcactc tttttttatg aaatcaaata tattttcatc atttcaacaa
    13501 tgaaaaagaa gtttatcata ttttgagaca gtttgttttt atttgtttaa ttcatatttt
    13561 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt agaattaact
    13621 caactctaat attcacttaa cttaagtggt ttattatatt attaaagaga agtaaacgaa
    13681 aggtagatta aggaggctaa attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    13801 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaata
    13861 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    13921 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttactta gcagttatag
    13981 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14041 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14101 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14161 tacacaagaa atatatagaa agatgctaga tagtgcttcg tttcctggga aattaaataa
    14221 tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa ttggtatagc
    14281 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14341 aaaaaatata agagcgctta cagtgtcaaa tcaaatagat tatagaaaat cagattatat
    14401 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14461 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14521 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    14581 aaatggaatt agagaagaat ttatagaaag attatctacc ataaataaag attatccaat
    14641 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    14701 aaaattaagt tgggaggtat tgtagaaatg gcttatgtag cagttaaagg tggagaagaa
    14761 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    14821 atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    14881 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    14941 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15001 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15061 ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat attaaatgta
    15121 gaattattag aagaagatga aaaaaagaat agagatttta gagatagttt tataaatgaa
    15181 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15241 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15301 agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt gactagaggt
    15361 gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg tgatgaacat
    15421 ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca tcccatctat
    15481 gaagatgaag aaataataat aggtgatatg ctggttactg aatgtgaaat agtttcaaaa
    15541 gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa taagatgtct
    15601 ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat ggccatacta
    15661 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt tgtattgctt
    15721 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    15781 gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt aggtgatata
    15841 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    15901 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    15961 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16021 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16081 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16141 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16201 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16261 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16321 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16381 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16441 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16501 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16561 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    16621 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    16681 aggaggtact tggaatgagt aaaataaatg tctctaaaag attagaaaat atagacaagc
    16741 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    16801 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    16861 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    16921 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    16981 tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17041 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17101 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17161 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17221 gaatagatca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17281 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17341 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa cttaagattt
    17401 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17461 taatgaaaaa tggaagtgtg attgagtctg gtcttacaga ccaaataatg gaggacccac
    17521 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    17581 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    17641 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    17701 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    17761 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    17821 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    17881 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    17941 ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta tgatatggca
    18001 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18061 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18121 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18181 gaaaaaatgt tggaacttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18241 gagtttatgg aaactgttat agataagacc tttactatgg caaaagggac tataagtgaa
    18301 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18361 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18421 aggattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18481 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagcca
    18541 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    18601 gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    18661 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    18721 aagagtataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    18781 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    18841 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    18901 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    18961 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19021 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19081 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaaaaaatt gggtgttcta
    19141 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19201 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19261 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19321 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19381 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggtttta
    19441 aatactattg taaatggaaa aacagtttat aaaagggagg tataaaaatt gctaacagag
    19501 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19561 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    19621 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    19681 tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    19741 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    19801 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga agatgtaaag
    19861 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    19921 ggatatacag ataatatata ttcaagtcta tataaagaat tgtttcatag aggaaacggc
    19981 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20041 tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag cgctattgaa
    20101 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20161 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggctca
    20221 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20281 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20341 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20401 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa aagtacactg
    20461 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20521 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    20581 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    20641 agtaaatcta atattgataa aaaagttaaa gaagttctag acgttgtaga attaagtgga
    20701 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    20761 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    20821 gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa actaggcatt
    20881 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    20941 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21001 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21061 aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc taaagataag
    21121 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21181 gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21241 aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21301 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21361 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21421 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21481 gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt cttatagcat
    21541 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    21601 ttatatattc atacgcaata acaagaacaa atataaagtt taaagatttt tttaaaacta
    21661 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    21721 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    21781 gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    21841 cactttcgat ggctgattat agactttatg aagcagcaga tagcttgggt gctagtgaat
    21901 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    21961 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtc ggaggaaact
    22021 acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22081 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22141 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22201 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22261 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22321 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatact
    22381 taagcagttt gaaaatatca actttaacag caatatttgg aactatattt gtatttttta
    22441 gtgcttatat aatagaaaag gtggataagt ttcctaaatt aagacaaaca gcttaccttt
    22501 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22561 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    22621 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    22681 tggataaaga gtatgaatta gttgcaaaat ctataaatat tccattctat aaagtatttt
    22741 ttaatataac agttccgatg tctataacat caatacttga aatagtagta tattattttg
    22801 taaattcaat ggttactgtt tcagcattaa tatttttata tatacctcaa actcagactg
    22861 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    22921 cagtattagt gctattaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    22981 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggaaaaaat
    23041 gaaatttaaa aagctagttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23101 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23161 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacatttaa
    23221 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23281 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23341 aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa aaggttctga
    23401 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23461 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23521 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    23581 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    23641 aaaagcatgg cagtatatgg ataagttgca tgagaatatt ggtgtataca ctcaatctgg
    23701 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    23761 tagaggtata aaattaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    23821 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatataa aagaagcatc
    23881 aaaagtattt ttagattggg ctataagtaa agatgcaatg aatgaatact ctaagaacta
    23941 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24001 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24061 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24121 atggagtaaa aagaaaaaat ttttttaagt agattttatt ataataatat aagttgagaa
    24181 taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt ataccattca
    24241 gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat actacagttt
    24301 aaaatagtat attttaattt cattattaaa gtatagatgt tatatataaa gtaaataaga
    24361 tattaaataa ttataaacta aagtaaataa aatattgagc gattattaaa aagatatata
    24421 tatatatttt aatttgagca gtttgttata tattataaaa aaagagaaaa ataaaaggta
    24481 aattgtttct ggataactta aaattggaag taagaatatt taactagaga agaattattt
    24541 aagttcaggt tagctctagg agtagctgat tttataggag caatagattt tatggatgat
    24601 gatataaaca tgatagcaat tagaccagaa gatataaggg tagaatttaa tagagatttc
    24661 aaagaaatta tagacctcaa actcagaccg tatcgatata aaaactaaag ctagatgata
    24721 tagtgttgta tataggacca tcagctgtta tggaagtatt agtgctttta acaaatataa
    24781 tagtaaaact tttgtatgaa ttgataataa aaaaattaaa aaccaagaca taaaaatggc
    24841 aacaaaaaga agttgaataa gggaggtaaa aatgaaattt aagaagttag tctctttggg
    24901 aatattatca acacttttat tgagtgtaat aactggatgt agtaatgttt catcaggttc
    24961 aaacttaaag tctaataata agacaaaagg agagttaaca gtttatacag caattgagga
    25021 agatagtata gatacatact tagctacatt taaagagaaa tatccagata ttaaattgaa
    25081 tgtacttaga ttatcagtag gaggtataac agcaagatta ttagcagaaa aagataatcc
    25141 taaagcagat gtgttatggg gtgttgcagc tacaagtttg ataattttag atgacaaaga
    25201 aatgctagag ccatacgctc cgaaaggctt tgataaggta aatcctgcat ttaaagatga
    25261 taagaaagtt ccttcttggg ttggaataga tgcttggatg actggtataa cagtaaatac
    25321 taaagaatta aaagctaaaa acatatcagc tcctaattct tatgaagatt taataaagcc
    25381 agaatataaa gatcttatat ctatgcctag tccagcttct tcaggaacag gatatttaac
    25441 agtatcagaa ctcatacaaa ttatgggtga agaaaaagca tggcagtata tggataaatt
    25501 gcataaaaat attggtgtat acactcagtc tggttcagcg ccagctatat tagcagcttc
    25561 aggagaatat cccattggta tatcttttgg aaatagaggt ataaagttaa aaggtgaagg
    25621 ttatccagta gaagtagtat ttccaaaaga aggttctgga tgggagatag aatcaaatgc
    25681 acttgtgaaa aaagataata taaaagaagc atcaaaagta tttttagatt gggctataag
    25741 taaagatgca atgaatgagt attctaagaa ttttgcagtt actacaattg acacgggaaa
    25801 tccggtgcca gaaggatttc caaagaatcc attagaacag atgatagata atgatttaaa
    25861 atcagcagca agaaatagag aggctatact aaataaatgg atagaaaaat atgatggaaa
    25921 gacagaaaaa gagagctaag ccttacaata aaatttagta caaaaagcgc ctagaatata
    25981 agttttacat ctaggctctt ttgatatatt tatttaaaat gcatatttct atataaaaat
    26041 aaatagttta gtaccttgat taaattaaat aaaaacctta aaataaagag atgtaaagaa
    26101 aaactaaaaa cgaataaaag gtgaatttat atgataaaag aaataatagt agtagagggt
    26161 agagatgacg ttacagcagt taagagagca atagatgcag aacttataac tacaggtgga
    26221 tttggatttc caaaaggtgt aatgcagcgt ataaaagctg ctaatgaaag acgtggtgtt
    26281 ataatattta cagacccaga ttttgcaggt gaaaaaataa gaaaaaaaat agcatcagaa
    26341 gttccaggat gtaaacatgc atttctacca agagaggaag ctaaaaagga tggagatata
    26401 ggtattgaga atgctactcc tcaaagtata atagcagctc tgaataaagt tagaactgaa
    26461 aatacagaaa aaagagatga atttaagcaa gttgacttaa ttagaaatgg tcttataggt
    26521 aatgaagaag catcacttag aagagatgca cttggaaaaa tacttggaat tggatatgga
    26581 aatgcaaaac aatttttaaa tagattgaat aattatggag ttgaaagaga agaatttata
    26641 gatgcattaa aacaaatcta aacgtaatgt ttaatgtatg aaaaatatgt atataaaata
    26701 aattgtggaa ttacggttaa tttaagataa attatttata atatatacct tattttaaat
    26761 caatgtaaat acatataaat aagtataatt aataaaatta aatatcaaaa taagttaaaa
    26821 aggagtaaat aatggataga ctatcttcac ataatgcaac aaaggaagta gtacaaaaat
    26881 ataattttaa attttcaaag tctttaggtc aaaatttctt gatagatagt aatataattg
    26941 ataaaatctt atctggagct agaatcacaa gaggagataa tatcatagaa gtaggacctg
    27001 gtataggaac tcttactcgt gagatgggta aaatagcaga aaaagtagtg gctatagaaa
    27061 tcgatagaaa tttaattcct atattgaaag ataccttgtc agatttagat aatacagagg
    27121 ttgtaaatca agatatacta aaagtagaca tacaggaatt agtaaaagat aaattaaatg
    27181 gtggaccagt taagcttgta gcaaatcttc catattatat aactactcca atagttatga
    27241 aatttttaga ggaagacata cctgtaacag acatagtagt catggttcaa aaagaagttg
    27301 cagatagaat gaatgctatt ccgggtacta aagattatgg ggcattatct atagctgttc
    27361 aatactattg tgatacagag attgtggcaa aagctccaag acatatgttt ataccacaac
    27421 caaatgtaga ttccacagtt ataggtttac atgtaagaga taaaagaaaa tatgatgttc
    27481 ataatgaaga catatttttt aaaacagtaa aagcatcctt tggacaaaga agaaaaactc
    27541 ttcttaattc gttgggaggt cttggttttc ttaataaaga tgaaataaga gaaatattaa
    27601 aagaagctaa tatagacgaa aaaagaaggg gtgaaactct tagtatagaa gagttttcag
    27661 ttctttcaaa tataataaat acaaaagtat cttctaaata gaaggtactt tttttaattt
    27721 tgttaacata tattgtttag agaaactgtg ttaagggggg attgcagtgg gttctaaaag
    27781 aaagtttaaa agagactata taatactaga agctaaagac ataaacttta gatacaaaga
    27841 acgagtactg ccaaaagctt ttgcaaaagt tgaagtaaat gatgaaaaag caattattgc
    27901 attatatgta gaaaatctaa aatatgttaa agatgggtat aaagcagtag caataaaaga
    27961 tgaccatgga ttaatagact taggaaatat aattttaaat gaacaaggaa gaggtgaatt
    28021 tgttttagat ttagaagatt ctgatgatga gataaaaggt attgcattat tgtatgaaag
    28081 aaatatacct ttaattggat ttaagggaaa taagcttgaa aattatgagg acatattatt
    28141 tcaagaggta gaggaagaag aatatgaaga atatgaagaa tatgaagagt tggaagaaga
    28201 cgaagagata gaggataatg aggaaagtga tagagacgaa gatagtgaag aagaatatat
    28261 tatagaagaa tatgatgaag atgagtatga agactattgg gaagatgatg aagatattga
    28321 agaaggagag ttaaaagcaa gaacggaagt agaagattct ggtgatgagg aaatagaaga
    28381 gttcaataat tataaggaaa tagaaaatta taatagggtt aaagtacagg aagaatatat
    28441 agacatagaa tatgatgagg aagaagagga agaagaagaa gtttttgaag taaatagaga
    28501 agaagatagg tttgtttcct caaaacctac taaatcaaag aaagttcaaa aagatcagaa
    28561 taatattgaa aaaataaaaa gagctaatgg agtaagtcaa ggagagagtg gaaataaaca
    28621 aaccagtaca ttacttatgc caagacaaat aaaaaaaggt ttgaaattat ttaaagaagt
    28681 aaaacctttt gtaacagatt atatagataa gacaagatgg tggaaaatag agataaatcc
    28741 tacaacactg tgtggatata caatgcctta tttaggatat ttaaacgtat tgaattacac
    28801 tatgtatagt gatgcagttt tacattcata taaatacaga cactatttat ttggtgttca
    28861 gtatgatgaa tataataaga gaaaacatta tatttatgct atcccaggaa atagaaatga
    28921 acagccagac aatggaagca caggattttt aagatatcaa gcttgtgata atagaacaaa
    28981 tagtttagga tattggctat gttttataga ttctagacaa agaaatatag ttaggtaaaa
    29041 ataataaaaa tgaaaaaatt tagactcaat tcttatttta ctgaaaaaat taatgaaatg
    29101 tagtacaaaa ctttaaaaac tattgataat atttaaatat tacactacaa tatatcttga
    29161 aatacctatt atattatgga ggaaaattat gataaacgag caaagaattt taaatgagtt
    29221 tcttgaatta gtacagatag atagtttatc tttaaaagag gggaatgttg ctaaagtatt
    29281 agttaaaaaa ttagaagaaa taggttgttc agtagtaata gacaatgctg gtgaaaaagc
    29341 aaatggagaa actggtaata taatagctac attaaaagga aataaagaag gaaagaaaat
    29401 attatttagt tcacatatgg atacagtaac tccaggtata ggggtaaaac ctatagtgga
    29461 tgaagcaaat ggaataataa aaagtgatgg tactacaata ttaggttctg atgataaagc
    29521 aggtatagca gctatattag aaggcttaag atatataaat gaaaataaca tagaacatac
    29581 tgatatacaa gttgtattct ctatatgtga ggaatgtgga ttagttggag ctaaaaattt
    29641 agactatgga aaaatagatt ctgaatatgc atttatatta gatagtggtg gttctccagg
    29701 tgaaataata gtaaaagctc ctgcacaaga tgtaataaat gttaagatat taggaaagac
    29761 tgctcatgca ggtcttgaac ctgaagctgg aataagtgct ataatggtag ctgccagagc
    29821 aatagaaaat atgaatttac ttagaataga cgaagaaaca actgctaaca taggaattat
    29881 aaacggtgga acagctacaa atatagtaac aggagaagtt aatatagttg cagaagccag
    29941 aagtctaaaa gaaaataaat tagatgttca aactaaacac atggtagaga cttttgaaaa
    30001 agctgcaaaa gattttggag ctcaaattga gatagatgtc aatagagcat atacaccaat
    30061 agatgttagt gaggattctg aaataataaa acttgcaaag aaggcttttt caaacttagg
    30121 tatagaaggt catactgaat ctacaggagg aggtagcgat acaaatatat taagtaagaa
    30181 tggtatcgaa gctataactt taggaattgg tatgaaaaat gctcatacac taagtgaaca
    30241 tatagctata aaagacttat atgactctgc taaaatggta gtggagataa taaaggaagc
    30301 ataaatttaa ataatagtgt ataatatata tatcgcctag cttaagctag gtgatttttc
    30361 attttataga agattgataa tctctattga aagcaaagag tatacatttg catataagag
    30421 agtgtttaat ttatgttcag atgtaggttc ttaataatat ttagattgtt tcataaggga
    30481 ggaataaata tggaaacaag gtatgaagaa gcaaataaaa taacaataca atcaatactt
    30541 tggaatgtag tattaacaat aattaaggtt atagctggtg ttattggtaa ttctagcgct
    30601 atgattgcag atggattaca ttcagcatca gatattataa gttctatagg agtactaata
    30661 ggcaattatg tatcttctag acctggagat agagagcaca attatggtca tgagaaagca
    30721 gagacattag tatcatttgt gttatcaata ttgcttattt tcgtttctat aacaataggt
    30781 atagaggcaa ttaaatcttt atttaactta gatgcattaa gtgttccatc aatattacca
    30841 ttagtagtat cagttatttc aatcctgata aaagagtatc aatatagaat aacaataaaa
    30901 gttgctaaaa agataaattc accagcctta aaagctgatg cttggcatca taggtcagat
    30961 gctttatctt cagtagctgc ttttataggt ataggtggtt ctattttagg gtttaagcca
    31021 ctagacccaa ttgcgtcaat tgtagtggct atatttgtag ctaaagtagg aattagtata
    31081 cttataagtt ctgttaatga attgatggat gtttcagttg acgaagaaga gataaaagaa
    31141 ttaaaattta ttgtagcaga tacagaaggt gttaaaaatc ttggtgatat aaagactaga
    31201 aaacatggag ctatggcata tgtagatttg actatatgtg tggatgaaaa tttgacagtt
    31261 aagcaaggtc atgatattgc taccaagctt gaaaaacata taataaaaca tatggaattt
    31321 gtaaaaggca taacagttca cgttgaacca tgtactaatt gccaaggaaa taagtgtaat
    31381 aattaattaa aacttaaaaa aaagtgtaaa aagttatata attgttttat gattataaac
    31441 tctaggggtg gttaaatgag caagaaaaga aaactaaaga aaaaagctgc aatattaata
    31501 ggagtagttg ttttctttat aatatacttt atatccttta gaataggaat aaatatggaa
    31561 aaggataaaa gtgaagtgcc aaagaaggta gaaaaagaag aagaagataa taggtcttta
    31621 aggactcaaa taaaagaaaa gaaaaaggtt tatatatcag acaaaaatat agaaaacata
    31681 aaaattgaag aagatatgtg ggatgaaata aagttatttt tctcagattt taagaaagta
    31741 agaaatacta cttcttctta cgaagcagtt tatgaaggtt attctgatga tggagttaaa
    31801 ttttctactg accttaactt ttttagagta tatactgtaa ataaagaaga atattataaa
    31861 gtaccagtta gttctaagga tgcttttaag aaacttttag atgagagtat atatacttca
    31921 tttgaccttt taagtcatta taaagattgg aaagaggtta ctataactca tggaaaagaa
    31981 attaaaaaag ttcatagatg gaaatttgat gatttagcac ataagatgat ttcaaagagg
    32041 gttgtaggaa aagttcaacc tgagaaaagt aaggaaagaa gtgactataa ttttactata
    32101 aatataaaag gtgataattt tgatgtaaaa gttgatacaa tgggaaaaga ttatgtaaaa
    32161 gttacttcta agaaaaatgt atcttactat gaggttcata caagtttatt tgattacctt
    32221 aaaaatgaga tatttgaaat tggaaagaaa taagacttta agaaggctat caaattagat
    32281 atttgatagc ctaaattatt tttatatatt atttttcaac caacaagtct gcaaccttat
    32341 aaattggacc agcaccagca gttattacta aatcattatc cttaacattt tcacgtaagt
    32401 attttactat atcttcaaat tcttttatat agatggcatc tacattgttt tgatataatt
    32461 tctcaaccaa gtccttagaa tgtatatctc cagggtcttt ttccctagca gcatatatat
    32521 ctgttatgat aactttatca gcagcataga atgcctctga aaattcacct aaaagagatt
    32581 ttgttctagt ataagtatgt ggctggaaga tacaccataa agttgatttt tttaattttt
    32641 tggcagcagc taaagttgct tttaactcag ttggatgatg tgcataatca tctactacta
    32701 aagcattctt ataataccct ttagtttcaa atcttcttcc gacaccttta taaattttta
    32761 tattttttct tatagtctct aagtcaatac cagaaacaag agctgccatt atagcagaag
    32821 aagcattgta tatgttatga agaccatata cagaaagttc aaattctcct aaatctcttc
    32881 cttcatattc tattctaaat attccatgac cactattgtc aaagtgaata tcttttatta
    32941 cagcatcatt gtcagaatct ctaccgtatt ttataatagt agctttaaca tcatacaata
    33001 tgtcatcaat attttcatca tcgccattta ttatgaaata gccttctggg ggtaataatt
    33061 ttccaaattt gttaaaagaa gccttgattt catctattcc agagaaataa tcaagatggt
    33121 cttcttcaac atttaaaact atagatatct ttgggttaaa attaaggaaa ctatctacat
    33181 attcacatgc ttcagttata aagtggttgg aattacctat cttaacatta ccacctatca
    33241 tacttaagtt accaccaact agtatagttg ggtctaagtc tgcatattca aagattgtag
    33301 ataacataga tgttgtagaa gtttttccat gtgttccaga aactgctata gaatttttat
    33361 attccctcat gatttggcct aaaaaagtcg ctctgttcat taccaattta tttttttctt
    33421 tagcagccac aagttcttca ttgtctggat gaactgctgc tgtataaaca accatattta
    33481 catcatctga tatattatct tttttctgac caatataaat agttgcccct tggtccctaa
    33541 gtttgtctaa taggtatgat tcgtttgaat ccgaacctga aacttgataa cctttattga
    33601 tacatatttc agctaatgca ctcatgctga taccgccaat tcctataaag tgtatattca
    33661 taataaaacc acctttcttt ttaattaagt cctacaatat gaactattta tgtggtataa
    33721 taatataatg ttcaatattt tataattaat atgcatataa tattatatgt attttatttt
    33781 tgaatataat aaaacataag taatattata ccacaataag aattgttatg cattagttag
    33841 tttaacgtta tataaaggta cttgtaggag gaaataataa tgaaatttaa aagaacagag
    33901 agaattggag caatagttaa aatactgtct gataatccaa ataaaatata tacattaagt
    33961 tattttacaa atcaatttaa tgcagcaaaa tcaacaataa gtgaagattt attagttgta
    34021 aaaaatgtat ttgagaagct acatttaggt aaagttataa ctatctcagg agcagcagga
    34081 ggagtaaaat atatacctaa aacttctata gcagaaaatg cagagttctt aatggaatta
    34141 tgtgagagaa tatgtgacaa gtctagaata ctatctggtg gttttttata cttgattgac
    34201 ctaatttatg accctacaat agctgctaag ataggaaaga tattcgcatc aaatattgag
    34261 tatgtagatg cagattatgt tgtgacaatg gaaacaaaag gtatacctat ggcacttatg
    34321 actgcaaaag ctatgaattt acctctagtt ataataagaa aagatataaa ggtatcagaa
    34381 ggtcctacac ttagcatgac atatgtgagt ggaaacagct ctaaagttga aagtatgagt
    34441 ttgccaagaa aagctcttaa accagatagt aaagttataa ttatagatga tttcatgagg
    34501 ggtggaggta ctataaaagg aatggtagac cttatgaatg aatttggtgc agaggttata
    34561 ggtactggag tattcatatc tacaacgaat ccttcagaaa aaatggtaaa agattatatt
    34621 tcattgattc aattggatgt taatggagat aaaatagtag tagaaccaaa cttaaagact
    34681 tttaaagatg aatatagaaa tgaagattta gacgaagaag aagattcaga atttgaattt
    34741 gagatagatg aagattaata aaaaaaatat ttagaaaaat atgtaaaaat aaaaagagga
    34801 tatccctagt tgttcataga attatttaaa aaacagttta tattaattta ttccagcgat
    34861 aataatttta atataaatta atttctgtca aaatcaaagg gggatatttt ggtatgaaga
    34921 taactgacgt aagagtaaga aaattaacag aagaaggtaa gatgaaatgt atagtttcaa
    34981 taactttcga taatttgttt gtagtgcatg atataaaagt tatagaaggc cataatgggc
    35041 tatttatagc aatgcctagc agaaaagtag gcgaaggaaa tttcagagat atagctcatc
    35101 caataaatgc agaaatgaga caagttttag aagatgcagt tttacaagca tatcatgaag
    35161 cattagttca atgggaagta gctgctgaat aataaatata taaaatggag agagcccgaa
    35221 catttaggct ctttttaaat gtagaatttc tattaaaata tgtacttttt agtaaaaaag
    35281 tgctatatta ttagtgataa tgtatatgtt gtatacaaat tgttagataa atgtattata
    35341 atgtatattg attaagagaa taatacgaat aaaattaaat tagattgaat ggagtgtatc
    35401 aatgaacttt aaagctataa tccttgctgc tggcaaagga acaagaatga agtctaaata
    35461 cccaaaagtt atacataaag tatgtggaaa agaaatggtt aaccatatta tagatgtttc
    35521 aaaaaaatca ggggtaaaag atactgtcgt gattttagga catgaagctg aggtagttaa
    35581 agaaaagctt gcagaagaaa ttataatagc catgcaaaca gaacaacttg ggacaggaca
    35641 tgctgtaaag atggctaaag aatatattaa tgatgaagat acaatagttg tactttgtgg
    35701 agatactcca cttataaaag aagagacact gaagagatta tttgaatatc atatagaaaa
    35761 taaatatcat gctacagttc ttacaacaag agttggaaat ccaacaggtt atggtagaat
    35821 aataagagat aagaagggtg accttcttaa aatagtggaa caaaaagatg caaattcaga
    35881 agaaaagatg ataagtgaga taaactctgg aatttattgt tttaatggaa agagtcttag
    35941 agaagcacta gatttgctaa ataataacaa ttcacaaggt gaatattatt taactgatac
    36001 tgctaaaata atgagagata aaggattaaa agttggtgca tttgcaggtt caactattga
    36061 agaacttatg ggcgttaatt caagagtaga attatcaaag gcagaagaaa ttatgagacg
    36121 aagaataaat gaatctcata tggtaaatgg agtaactata atagacacta actctaccta
    36181 tatagaatct gatgttatga taggcaatga tactataata tatccaggtg ttatgttaca
    36241 agggaaaaca aggataggtt cagattgtat aattggaatg aactcatcta taacaaattc
    36301 agaaataggt gatggtacag aaataaaaaa ttcaactata attgatagta aggttggaga
    36361 aaattctaat gttgggccat atgcatactt aagaccaaag agtgacttag gaaataatgt
    36421 aaaaataggc gattttgtag aagtaaaaaa tgctattata gaagatggat caaaagcatc
    36481 tcacctttct tatataggag atgcacatgt cggtaaaaat gtaaatattg gatgtggagt
    36541 agtatttgta aactatgatg gtaaaaataa atttaaatca gtagtaaaag acaatgcgtt
    36601 tataggttca aattctaatt tagttgcacc agtagttgta gaagaaaaag gatatatagc
    36661 aacaggttca acaataacac atgatgtccc agatggtgct ttggcaatag caagagaaag
    36721 acaagttatc aaagaaggat gggtagaaaa aaagaatcaa aaagacgacc aaagtaagta
    36781 attatgtagt ttattatttt ggctaaaaaa tttagctcga aattattata aattatattc
    36841 attagtattt taaaattttc aggagggtta cataaatgaa tactagcgga agcgagatta
    36901 aaattattgc aggtaattca tcgaaagagt tagcacaaaa aatagctgac tatataggtg
    36961 tatcagtact agattgtgaa gtaggtacat ttagtgatgg tgaaatatgt gtaaacatga
    37021 atgagacagt aagaggttgt gatgtgtttg tagtgcaatc tactaacagc ccagtaaatg
    37081 ataacttaat ggagttatta atcttaattg atgcgttaaa gagagcatca gcaggtagaa
    37141 taacagcagt cattccttac tatggatatg caagacaaga cagaaaggct aaggcaagag
    37201 atccaatcac tgctaaatta gttgcaaatt taataactgc tgctggagca gacagagtat
    37261 taacaatgga tttacatgca gctcaaatac aaggatattt tgatatacca ttagaccact
    37321 tactaggtgg aacaatatta gcaaattatt ttaatgaaaa gaaaatagaa gatttggtag
    37381 tagtatctcc tgacttagga agtgttacta gatcaagaaa atttgctaat actttaaatg
    37441 gagaagttcc aatagctata atagataaaa gaagaccgaa agctaatgta tgcgaagtta
    37501 tgaacttaat aggtgatgtt aaaggtaaaa atgttatact attagacgat atgatagata
    37561 cagcaggaac aatagttaat gctgcaaatg cacttaaaga gtttggagca aaagatgttt
    37621 atgcttgttg tactcatggt gtgttatcag gtccagctat tgaaagaatt gctaattcag
    37681 aaataagtga gttaatagtt cttgatacta tacaacttcc tgaagagaaa agaatagata
    37741 agataaagat aaaaacagta gctcctttat ttggagatgc tataagaatg atattctcta
    37801 atgaatcagt aagtaaatta ttctaattta ttgtatttat gagagttgtt taaaaggata
    37861 taaacctttt gagcaactct tttttgattt tatacttatg gaatgttatt tttttcttgt
    37921 tagaaaggta tatattattg taaaaagaga ttatagttta aaattatgta tatttaagta
    37981 aaataaactt aataaagggc atattattca aaatattatg aatataaaaa atattacaaa
    38041 aaagtattga aaaaattcag attatgtagt attatgtaaa tataaataaa gaaagaggtg
    38101 tttccaatgg gaaggttaga agatgaaccc ctaataatta aatgattcag tcacaatcta
    38161 aagtgaatat aaaaatctat tgaaagttta tttataagaa ttgaaaatag tatctattct
    38221 atctttagtg tttgtgagag ggttctggat tatataagat tactgtaaag agtaattttg
    38281 cacaataagc atagctgtaa atgaagagtt tcgtttatat aatgattgtg gctgaatcat
    38341 aactataata gatagaatgt ttataataga tgattttata aaatactata ttgtaatagc
    38401 ttttagcttt caaataaata ttagcaaagg atggaaatga caatgataaa actaaatgat
    38461 aatttataaa tatctcctta cgatatataa taattgtagg gagatgtttc ttttttttgt
    38521 agagtataat ttttctagaa attctgtttc aatatgtaaa aagttggtat atataaatag
    38581 tataaacagt gttgctaaaa tatggtagat attatataat ataagtggat atgtatatac
    38641 attatgagtt atataatttt gaatttaata aaatagaaac gttgatttat gttctgtaat
    38701 gtgggcacct tagagcatat tgagttagtg gtgcaaccgg ctatgaaatt gtatttattt
    38761 atagatacta tcattttcat agcttttttt atttaaaaaa tggttatgtg ttagaaataa
    38821 atttggttag tacgtttaaa taattttact tagattttta accagatata ttataagtaa
    38881 gtattaatta aaaaatttaa gggggaataa aaatgaagtt aaaaaagaat aaaaaaggtt
    38941 tcactttagt ggaattattg gtagtaattg caattatagg tatattagca gtagtggcag
    39001 ttccagcttt atttagtaat ataaacaagg ctaaggtagc aagtgttgag tctgattata
    39061 gttcagttaa gagtgcagca ttatcttatt attcagatac taataagata ccagttacac
    39121 cagatggtca aactggttta agtgttttag agacttatat ggagtctctg cctgataaag
    39181 ctgatatagg tggaaaatat aaattgatta aagttggtaa taaattggta ttacagatag
    39241 gtacaaatga tgaaggagtt acattaacag aagcacaatc agcaaaatta ttgagtgata
    39301 taggtgaaaa taaaatatat acaagtgtta cagcagataa tttgggaaat ccattaacaa
    39361 gtaatacaaa agtagataat aaagttctat atatagtact tatagataat actgtgatgg
    39421 actcaacaaa atagttaaaa ctatattttt ataaaaatat tgtaaattta atgaaaaata
    39481 taatataaaa gacaggaaga aaatcttcct gtttttttat actattttct ttaatgttca
    39541 attatgaatg atgatataat tgagatagta aaggagtgaa acctgtggct aaaaaagtta
    39601 gaataggaga caaactggtc gaaaaaggat atataacaga agaccagctt aaatgggcac
    39661 tatcagaaca aaaaaacagt ggaaaaagac taggagaatt tctagtacaa gaagggctta
    39721 tagatagtaa cttactaata agtgtattaa aagaactctt ggatatagaa agtatatttt
    39781 tagaagggac agaaatagac actttagcta caaagatggt tccagaaaat atatgcaaaa
    39841 gatatactgt atttccattt aaaattgatg gaaataaaat atgtctagcc atgtcagacc
    39901 cacaagacag agaagcagtt caagatgtaa ggcgtatgtc tggtaaagac gtagagatat
    39961 ttatatcaag tacagaagat ataaacaaag caataggtca tgcttatgca cattcagaaa
    40021 taaataaggc aatgactgag tacaacaaaa atagaactgg tggagtaaga gaaacagtaa
    40081 tacttgaaga agatgtaaat gctgctccaa tagtacgatt agtaaataac atactagaaa
    40141 atgctgttag aatggaagca agtgatatac atatagaaca aagtgaaaat tacatgcgtg
    40201 ttaggtttag gatagatgga atgttaagag aatatatgag gatgaactca gcaccatata
    40261 aggcagtaat cagccgtatc aagataatgt cagatataaa tatatctgaa aagagaatac
    40321 cacaggatgg tagaatctat ttaaaagtag ataataaacc catagatttt agggtttcta
    40381 caatgccaac taacagggat gaaaagatag ctatgagggt tcttgataag agtaatttta
    40441 tggttagtaa agaagtacta ggaatagacg aacatggctc taagatatat gatgaattga
    40501 taaacactcc ttatggtctt atattggttg ttggtcctac tggaagtggg aaaaccacta
    40561 cattgtattc aatgttaaat caactaaata cagaaaatag aaatctatta acaatagaag
    40621 accctataga gtatgaatta cctggtgtaa atcaatctca aataaatgaa aaggcagggc
    40681 ttactttcgc cagcggactt agagctttta tgcgtcaaga cccagatata ataatggttg
    40741 gagagataag agatactgag acagcagaaa tagcaattag agcttctctt acaggacatt
    40801 tagttttaag tacattacat gcaaatacag cagttggagc aatttcacgt cttttagata
    40861 tggatgtgga aagtttttta ataacttcat ctgttttagg tgtaatatct caaagactta
    40921 cacgaaaaat atgtgagcat tgtaaggttt catatgaagc agatattgga gaaaaaaagg
    40981 ctcttggtat tgatgtaaat gaatcagtaa ctatttatag aggaaaaggt tgtgaaaggt
    41041 gtaataatac tggatataaa ggacgtctag gtatatttga aatgcttgaa attactccag
    41101 aaataaaaga gttaatagat tcatctgcaa atcaaagaga aattttaaag atggctagaa
    41161 aacaaggcat ggttagctta aaagaggata tagttaaaaa ggttttaaat ggaaagacaa
    41221 cagtagaaga aatgattaga ataattttga tgactgatta aaggatgata ttatgaaaag
    41281 ttttaaatac acagtaataa aagaggacgg aaaaaagcaa actgatgtga ttgaagccaa
    41341 cgattttaat gaagctagaa atcttcttag aaagagaaat cttagagtta ttgaaatcaa
    41401 agaaagcaaa aataaaaata taagattatc tagtagaaag agaaaaaaag acttgggagc
    41461 tgaccagata agccactttt gtagacagtt tgctattata gtatcttctg gtataaatag
    41521 tatttcagga ttagaaacat tagctagaag gtcaactaac attacattaa gagaagaaat
    41581 taatcgtata gtggcagaaa taaaagtagg ttctactatt gcagattcaa tgctttcatc
    41641 aaaatcaaag tttccaaagt tacttggtgc tatggtagct acaggtgaag ctacaggtaa
    41701 attagatgaa gtattaaaaa gtatggcttc attttatgca agtgaacaca gggttaaaca
    41761 aaagcttaga aatgctgcaa catatccatt aatagttttg atttcttcat ttataatgat
    41821 ttttatattt acaacattta tggtgcctaa gatgattaat tcaattacaa cagtaggagc
    41881 aactcttcca cttataacaa gaattgttat ggggtttggt atgtttatga agaagttttg
    41941 gataatagta cttttagtta ttatattatt tatctatcag tttaaaaaat atttaaaaac
    42001 gtcagttgga agggctcata aagatagagt tataaacaag atacctgttt taggaaaagg
    42061 aataaattgt atggtagcta cacgattttc aagagcattg tatctatttg tatcaacagg
    42121 atacccatta gttcaagggc ttgattatat tatagatagt gtaaataaca ccatggcaga
    42181 gaagatttta gcatcagcta aagatggaat aacaagagga gaaggcttag cagaaaattt
    42241 agagagatat acgtattttg actctgtgtt ggtacagatg atagctatag gggagcagac
    42301 aggggaatta gagaatataa gtagacagat ggcagaattt tatgagtatg aatccgagat
    42361 ttatttaaat aggatggcct caatgataga gcctgtttta ataattgcag ttggtattat
    42421 agttgcgata ctggttgtat caatatttat gccaatgctt agtatatatg atgccatgta
    42481 ggagggaaag gacaaatgaa taaggtttat gtatcatatt atggcgattc tatttccata
    42541 gttgagggca gatataaaca cgataaggat aagttttatg taaaaaattt taatgtgttt
    42601 tcaatagagg atattgcaca agatttttct aaggaaaagt atgctctatt gagatacgca
    42661 ttagaaaatg ttaagttaaa atcaagacat gtagtttttt gtttaaatac aagagatgta
    42721 attttaaaac cccaaaaact tcctaatatg gacaaaaagg atttagatgg atttatgaag
    42781 ctagagatgg acgaaatgat ggctttggag tcagatgaat atgtattttc gtatgaagta
    42841 tctagtgaaa cacatgataa ttcagatggc gatgaatcaa gcttaaatct tatcattgga
    42901 gctatacaaa aggaagaggt aaatgcaatt gtagagataa tgcatgaatt tgatttagta
    42961 ttagatagaa tagatacatt atcaacagct tacttaagaa tgcttaaaaa tttagactac
    43021 aatgatataa tggtaaccaa tataagtgac aatagtacaa ttataaatat atataagaaa
    43081 gatactttat ttattagtga taatatacct ataaagataa ctgctaaaga tatggacatg
    43141 caattattaa atgtagtaga agaatcaaaa ggtcttatga attactattc gtcaagaaac
    43201 tttggtaagg ttacggatac tatattaata ttaggaaaaa gatattccaa taaattaatt
    43261 tatgaaactt ttagctctgc attttcaagc tatgtttctc aaggccttac tgagataatg
    43321 gatatatctg atatgataac tggagatata gatgaagatg atataaattc aattgtagag
    43381 cttttaggaa gtatgataga acctaaaggg aaaagagatt ttgaaaatat aaacttttta
    43441 ccaatggata tacttagaaa acagataaaa gctaaacaaa tgaaaaacat actgaaaatt
    43501 gctccaatag ttattttgat tcttgctata ccttatttat gtttaatttt ttcaaataat
    43561 ttagcaaata aggaacttga aaatgttaat ggtgaaataa gaaatattga atctgcatat
    43621 tatcagattg gaaatataga aaaaatgata cagagtaaaa gcgaagaaat aaaggtatat
    43681 gatatgttaa ttgggaaaaa ggtaaagtgg gaaaatttat tggatgatat agataaaaat
    43741 actccaagta aagttgaact aacaagttta tcaacaactt atcaaaatct tggaactaat
    43801 aataaagctg ataattcaaa aagtaataag actacaacag ataaatcaag taacaatgaa
    43861 acaaaatcaa gtacgaaaag tgatagtaat ggagataaaa cagataatga tttaaatgaa
    43921 gataagacta caaatgtgtc agaagcatta actggaaata gtgagactaa tagtaataac
    43981 acggataaag atgataaaac tccattgtat gataagatac caaatgttat aaatatagaa
    44041 ggaaatgcta aagattcctc ttatgtaggt caatttttat ataaattaaa gggattatac
    44101 tatttcaagg atgttaaatt acatagcata aaaagtaatg cagaagattc taactataca
    44161 tttagtatga cattatacct gaaagaaggt gtattaacta atgaataaag atatatttaa
    44221 aaaagatata aaagatatat ttaaaatgga agttagtcta aaagggattc ttataaaggt
    44281 tataatttta ttagctattt tatcatatgg ttattttgga ctttaccctc aatatcagaa
    44341 atatattgct acaaaaaatt ctgtaaaaca ggctactgaa aaattaaata tatataaaga
    44401 gaaaactgca cttatgccaa aatatgaaaa acaacttgaa gaagttgaaa gtgaattaaa
    44461 agaaaaaagc aaaaatcttt tatatgatat gcaagatggt ctttttttaa taggtttaga
    44521 taaaaaaatg aaggcttata atattgactt aactagttat gacgttggaa atgttaaaga
    44581 atacgaacat ttttatggta tatcaacaac aataagtgtt agaggtgatt atagaaatgt
    44641 caaaaaatta atggcttatt tagaagaaca aaaaaatgtt actcaaatac tagattatac
    44701 aatgacttca tatatagaac cagaaaaaga agaggaagaa acaacaaaca ataaggattt
    44761 aattaaaaat gtgaacataa ataaagatac aaaaatatac tgggtggaag gcgaaaataa
    44821 gtggcattta tataaggatt gtccattgat aaaagaagga gagattctaa aaaatgacaa
    44881 acttttagat aggtctacta attatctatg taatacttgt gtacttagag ctgcaacaga
    44941 aaaagaagca aaaaaagaag tcgtagctcc aaaggcagaa ggtgtagtag atgctacatt
    45001 taaatttata atgtacacta aagagaatac tacaatgata cttgatacag ataatccatc
    45061 ttcatggaga ccaggaaaat ataatccatt taagtctagt gctaaataaa gctaagtaag
    45121 ctttaaagat aggtgatgag tttgtacaaa aataatgaaa aaggtcttac tttattagaa
    45181 gtaataatag ctgtttttat actgacaata gttttaagta tttcttataa agtgttcaat
    45241 ggtataacat cagcagtaaa aaagcaacag attattacag atgctcaagt aaatattaat
    45301 ctaattaata agtatctaaa tagagatttg gaaaactgta aagagctaac taaaactggt
    45361 tcaggtaata attatgaata caatatagag atgccagata atgttgtaaa atatgaagtt
    45421 tctatagaaa ctaaaaaaaa tacagaggta tattctgtaa cgagaatcca aaacaataca
    45481 attgatacag aaaatgaagt tagagaagag ataatctata atcaaccact agttcaaaac
    45541 aataaagaaa tgaaggaaac accatttaaa atagaaaagc aaactggtaa atctatttat
    45601 actgtaagta tatactataa tgaatcagtg caagaaagtc ataaaaatag caatttaaat
    45661 aacaaaactt atacatttga tgttatgtca agaatagggt aataagaatt tattaaaaaa
    45721 taagtaggtt gataaaatat gagtaaaaag aatagtgaaa gaggattttc cttaattgaa
    45781 gtgttagtag ctatggctat tatgggaatt gtactatttg cattttttaa tattatcaat
    45841 actaataaca aagcaaacac taaaaatgac acagatataa cttccttaaa ctatgtccaa
    45901 agtgaaatag aaaatctaag agaaaagata aaaagtggag aatttgattt tgatagttta
    45961 gataaactag aagatggaac tgtcgtatat gaaaaattaa tagataaatc aaaaaaagtt
    46021 gtatatgata aagttcttag tgaaggtgac gtgagcttat atgatactcc atatgaaaag
    46081 attacaacaa taaaagatga agatggtaat ttaatcgaca aaggaaatat tacaaataag
    46141 ataaagacta ttgtagaaga taaaagtggt cagatatata aaatagctgt aactgggaaa
    46201 agcatgaatg actattcaag taaaaaagag gttaaaattg taactgaaat atttaaagat
    46261 aaataatttt acttgggggt gagattttga ggaagtggaa taaatttaaa agtgaaagag
    46321 gggcagcttt agttttagtt ttgattgtag tagcattgct ttctattgtt ggattgatat
    46381 tttcaaatca gatagctaat agaataaaat ctacaaagac tactaatgaa ggtatacaag
    46441 caaagtattt agcagagaca tgtgtggaaa atagtattga taaagcatat gaaaaattgt
    46501 atgatgagct tgagaaaatg gataatgagt ttaaaagtga aaatcaagaa aaatccatat
    46561 ctaggtcaaa attaagaaat atatcagatg aagattttaa taatcaagat gagaaaaata
    46621 ttgaagcaga aagacttgga tatatgaata atataaattt ctatttaaat aaagcttctt
    46681 cagacttgga aaaagctagt atggaattga aaaagctata tgatttagat atgttggact
    46741 atagagacat tgaatatgta gatgcaaata ttatttcaca tagagattct atcttagaaa
    46801 tttgcaagaa ttatacttct ggagatatta gtaaaataaa tgaatatatt ctgaaagaag
    46861 atatagactc aactacattg attgaggcaa agttagtgaa taatgatatt cttttgaaaa
    46921 tgtttttaga agaaaataaa attgaaaatg aacatttaaa ttcagccttt tcacatactt
    46981 ataaggcatt agataacatt tctttggcga tgcaaaatat gattgagtat agacatacgt
    47041 ttcatataga tgagccaaaa gtagaagtat ctaatgggat tccagattct caacaatatt
    47101 atgaactaat acaaaatcct ataataaata gtatggagta tatctggaat agtaaatggg
    47161 atacattaga aaacctattg gaaattttac caaatcaaac tcaaggattt aattctttaa
    47221 gagttcattt gagaaataat gtaagaaaat ttgagaagtt aagcgataat atatctagtg
    47281 gtaaaaaaaa tacagcaaaa aattttttaa agtataaaga gttattgtat gaaatatcag
    47341 accaatgtaa tcaactaaaa agtatgtctt atgagaaaat acctgttaaa tatgataata
    47401 tggctttgat aacaactttt gactatatac aaaatgagct tttagcagag ataaaatgca
    47461 gattaaaaga attaaaacca caagaaattg ataaaacaga aggtattact ataaaaatac
    47521 ctttttataa ggctgactac gacatgacaa aagaaggatg gcctaaatta aaagaaaatg
    47581 gttcaggggc ggagttaagc ttaatggtaa ctggtgataa agatggaata aaagaagtag
    47641 aagttacaga tggtaaaaaa aatattattg gtttaggtgt tgaagaaaat tcaaattcaa
    47701 agtataaggt tgacgccatt gtaaacttta atctaaatat agatacaaat gtagttggaa
    47761 attatgatat aaaagataaa attttgatta atcatgatat aagttcatac aaaaaagtaa
    47821 actaaatata agaagcaact tagtataaat aaataataaa ataattttat tatttactac
    47881 taaaaaaata agtaaactaa tagaagatat ggagagtaaa tattatgtat atttatgatt
    47941 tattagagca aggtataaga ctaaacgcat cagatataca cataacagtt ggaacaaatc
    48001 ctgtagctag agtaaaaggt ggatttgtaa aactaagtga acaaatattg acatcagaag
    48061 taacaatgca aatggcaaaa gacatagcgg gggaatctat ttttaaagta atagaagaac
    48121 atggagaagc tgatttttca gcttctctaa aaacaggaga aagatttagg gtaaatgcat
    48181 ataggcaaaa aggaaattac gcaatagcaa taaggactat aacagcagaa attccaacat
    48241 ttgaaaaatt aggccttcca gagagtataa aatcttttac tgaaaagcat aaaggattag
    48301 ttttagttac aggtccaaca ggaagtggta aaagtacaac acttgcaagt atgataaata
    48361 taataaatga aaaacaacaa aaacatataa ttacacttga agacccgata gaatatgtac
    48421 atcatcataa acaaagttta gtaaatcaaa gagaagtagg aactgataca gaaagttttc
    48481 attcagccct tagagctatt ttgcgtcagg acccagatgt aatactaata ggagagatga
    48541 gagaccctga aactgtttca atagcattaa cagcagcaga aacaggtcat ttagtatttt
    48601 caacactaca tacagtaggg gcagctaaga caattgatag aatagtagac atgtttcaac
    48661 catcccaaca gcaacaaata aaaacacagt tatctacagt ttgtgaaggt gtggtatcac
    48721 agcagttact tcctacagca gatgggaaag gcagaatagc agcaattgaa ttgatgtttg
    48781 ctactccagc aataaaaaac ttgataaggg aaggcaaaac atatcaaatt ccaaacatga
    48841 tacaaactgg agttaaagta ggtatgaaaa ctatggacca agatttaatg gaactttata
    48901 aaaatggaaa gattacaaaa gatatggcac ttagtaggtg tactgaccaa gagtttatga
    48961 caagaatgat aggtggagta aactacaatg gatattataa tcgttaatct atatgtattt
    49021 atagtgggta tagtgtttgg aagtttcttt aacgtctgta tttttaggat tccagaaggt
    49081 atatctattt ctagacctcc ttcccattgt atgagttgta atactagact aaagccaata
    49141 gacttaatac cagtgctaag ctatttgatg agtggcagaa agtgtagata ttgttatgag
    49201 aaaatttcta gtagatatgc tatagtggaa ttaattacag gaattttatt tttaacagta
    49261 tttatgttat atggaatatc tacatcaact atttattatt tggttcttgt gtctttactg
    49321 attataatta catttataga tattgaccac tttataatac cagataaaat attgattttt
    49381 ggagctatat tttctatgat ttttaacctt ttatttaaag aaattccagt taaggattca
    49441 attctaggtg tttttatttg tggaggaagt gtctggatta tagttctttt gattgaattt
    49501 gtaataaaaa aagagtgtat gggtggagga gatatcaaac tatttgcaat gctaggcctt
    49561 tacatgggtg ttaaaaatgg tcttttgaca gccttactaa gcgtttatgt aggagcagtc
    49621 tatggaatct gtgtaataat tataagtaga ataaaaggaa aggaatataa ttctgttata
    49681 ccatatggac cttttatatc tattggagca ttaataacaa tcttatgtgg aaagcaatta
    49741 cttgaacttt atttttctat ggttatatag atgatagtca agttaaataa agttttaatt
    49801 taaaaaaatg aattaaagta tttaataagg acaatattat tattaaccct attactaaag
    49861 tgctacaaac tatggagtat ataataataa tttccttatt ttttgcacat ttgataaata
    49921 aatctctatg tgttttttgt gatagagaaa agaaaaattt atataatacg ttttttattt
    49981 acataataac tgtagtttta tctgtattaa tatatctaaa atttgggatt agtgtagaat
    50041 gtgtacaata tatggtttta attccatttt taataattat atccattgta gattactaca
    50101 caatatatgt atatgatata acaatagtta gtggtataat agttcaaggt ataatttttt
    50161 tggccttttt caatatagct caatttgatt atataaatca tatatttgga ctattgatag
    50221 gctttattat accttatatt attgctaaac taacaaaagg attaggctat ggagatgtag
    50281 gtctctatag cttatgttgc tttgcattgg gaaataatta tgggttttat gtgatagttt
    50341 tatcatttat tttagcgttt ttgtttttta ttattttata cataactaaa atatttaaag
    50401 atgtagatat aagaaagcaa attcctttta caccttttat ttccttgtca acaattttaa
    50461 taatatttac agagtataat atatttaatt tttattataa tttgttatac aaatttatat
    50521 aacaggaggt aagaaatgta cgtagtagta gggttaggta atccaggaaa gaagtatgag
    50581 aagactagac ataacgttgg atttgatgtt atagatattc ttgctaaaga atacaatatt
    50641 agtgttacta aaataaagca taaagctttg attggtgaag ggagagtagg tactgaaaaa
    50701 gtattattag ttaaaccaca aacttatatg aatttaagtg gagaaacatt aatagatata
    50761 tataaatatt ataaggttga tttaagtaat atagtagtag tatatgatga tatagattta
    50821 gaagttggaa agatacgtat aagaaaaaaa ggaagtggag gaactcataa tggcatgaaa
    50881 tctataacta agtgcctagg ttcaaatgac tttccaagag tgagagtagg tgtatctaaa
    50941 ccagaagctg gtcaagactt agcagacttt gtactttcta gatttagaaa agaagaatct
    51001 gataacataa atgaagcatt agaaaaagcg gcagatgcta ttgactcaat aataagagaa
    51061 aatatagata tgtctatgaa caaatataat ggatagtgag agggagattt aaatgaatga
    51121 tgttttttta tatcctttgc aaaattcaaa agagtataaa gatataataa actgtataaa
    51181 aaatacaaaa ggctcattat tggttaatgg acttttacca gtacaaaaac ctcatatatc
    51241 gtattctata tttaatgatt tatcacgaca aatgattttt ataacgagta gtgatttaga
    51301 agcaaagaaa gtatacgaag atttaagttt ttatatggaa gataaagttg aatatttagg
    51361 ttttcaagat atttattttt atcacttgga tgcaaaggac agaaacgaag aagcaaagaa
    51421 attaaaagta ttgttaaaac tagcaaataa agaaaaaatc attttagtaa cttctattga
    51481 ggcagtttta agaaaataca taccaaaaca agttttgctc gatagtgtat ctcattataa
    51541 ggtgggagat agcttagact tagaaaagtt aactgagaaa ttagtttcat tgggatatga
    51601 aagagtttca aaaatagagg ggtttggtca atttagtata agaggtggaa ttattgatgt
    51661 attttcactt gaatatacaa atccaattcg tatggaatta tttgatgatg agatagattc
    51721 catacgaact tttgacgtat attcacaaaa atcaattgat aaattgcaac aattctctat
    51781 aactccatct agagaattta tttatcccga aaaaactaca gatgcattag ttaaattaaa
    51841 aaaagaaaca actaaaaata ctgatgaagg tacatttcaa aatatagatt atatatcaag
    51901 taagacttat tttgaaggtg cagagaacta tatagattat atttatccag aagaaaacaa
    51961 aagtatattt acttatttag cagatgatgc aatagtattt attaatgata taacgagact
    52021 taaagaaaga tgcgaaaatt atataaatga gtttagagaa aattataaat taaatcttga
    52081 aagaggtctg gctattaaaa accaaggtaa attgttatat cactatacag atttagagta
    52141 cttagtaaaa gacaagtctg tggtattaaa ctcgctattg ccaaagtcta taaataactt
    52201 ttctataaaa tctataataa attttgaaag tagagaagtt cctacattta atggaaaggt
    52261 agatttgctt gtagaagaat taaatcggct gaaatataat ggatacaaaa taattttagc
    52321 aacaaatacc ttagaaagag caaataaatt aggaaaagat ttattggaca aaggcttaga
    52381 aacgaccatt tctaaagata gagatattga aattaaatct tcacaagtta taattgtacc
    52441 tgcacacata aacagtggtt ttcagtataa atctataaag tttgttgtta taacagataa
    52501 cgaaatgata ggtgtttata aaagagcgtc taagactagc aataaaaaag ttaaaaaagg
    52561 aaagaaaata gaatcctttt tagatttaag tgttggagac tatgtagttc atgaaaatag
    52621 tggtgtaggt agatacacag ggattgaaca aattacagtc aatgctatta aaaaagatta
    52681 tatgaaaata gtatatcaag gtggagataa tctatatgtg ccaatagacc agatggacaa
    52741 agtacaaaaa tatattggtg cagaagtaga aaaagttaag ttaaataagc ttggaacaaa
    52801 tgagtggacg aaagcaaaag caaaagttaa aaaagaaatt gaagatatga caaaagactt
    52861 gattgagctt tatgcaaaac gtgaaaaaat acaagggtat aagttttcaa aagatacacc
    52921 ttggcaggca gagtttgaat cgttgtttcc atatcaagaa acagaagacc aattaaaggc
    52981 tatagaagaa actaaaaaag atatggaatc aagtaaggta atggataggc tagtatgtgg
    53041 agatgttggt tatggaaaaa cagaggtagc tattagaagt atttttaaag cttgtatgga
    53101 ccaaaaacaa gttgctgtat tggttccaac aacaatattg gcccaacaac attataatac
    53161 attcaaagag aggtttgaaa attatccttt gagagtagaa gttttaagta ggtttaaaac
    53221 accaaaacaa caaaaacaga ttattgaaga tgctaaaaaa ggattagtag atattttaat
    53281 tggaacacat agaataatat ccaaagatat aaatttacca aacttaggtc ttgtagtcat
    53341 tgatgaagaa caaagatttg gagtaaaaca taaagaagct ttaaagaaaa ttaaatctac
    53401 agtagatgta cttacacttt cagcaactcc tataccaaga acattacata tgtctttaag
    53461 tggaataaga gacatgagtg ttatagaaga acctccacaa gaaagacatc ctgtaataac
    53521 atatgttaca gagagtaagg aaagcgtaat tcaagatgag atagagagag aattaagtag
    53581 aggtggacag gtattttttg tatacaatag agttgaacac attgaagaaa tggcaagtat
    53641 gattcaaaaa ctcgtaccag atgctagagt ggctgttgca catggaagaa tgacttctaa
    53701 atcattagaa aatataatcc taggattttt aaataaagat tatgatgtac tagtctgtac
    53761 tacaataatt gaaactggta tggacatatc aaatgcaaat acaatgatta tatatgatgc
    53821 agataaaatg ggattggctc aactttacca attgagaggt agggttggaa gaagttcaag
    53881 acaaggatat gcatatcttt tgtatgaaaa agataaaact ttaagtgaga tagcggagaa
    53941 gagattaaaa gctatcagag aatttacaga gtttggttct ggttttaaaa tagcaatgag
    54001 agacctcgaa ataagaggag caggaaatat tttaggttct caacaacatg gtcatatggc
    54061 agttataggt tatgatttgt acgttaaaat gcttaatgat gcaattaaaa aagtcaaagg
    54121 tgaacctatt gtggaggaaa ttgatgttga aatagattta agtgtaaatg catacattcc
    54181 agataattat atcaaagatg agttgattaa aattgaaatg tataagaaaa tagcttctat
    54241 tgaaaacaaa gaagacatgt tggatataca agaagaactt gaggatagat tctctgatat
    54301 acctaaacca gtccagacac ttcttacaat agcttatata aaatcgttat gtaagatatt
    54361 aaaaatagaa aagataagac aattaaaaga tgaaatatta ttagttccaa taacaaagta
    54421 tagaactaaa cagaaaatag gttataatat agttacagaa ttagaagaat tattagaaaa
    54481 gatgtgcaag gtaaaataac ttgtaaattt aagaagggat gttttaaatt tgaaaaaagt
    54541 aataacattg gtaatagcta tgattttggt agtatctgtg actgcttgca gttctagtaa
    54601 aggagaaact gtggcaacag ttgaaggtac aaagatatca tcagatgaat ttaaaaaaac
    54661 tatagctctt tataaagatt ctatggaaca aacttatgga aaagatatat gggataagga
    54721 agttgaaaag ggtgtaaaat acaaggacaa gtttaaagat ttaattcttg accaacttat
    54781 aactacagaa gtaatatatt ctcaagctaa aaaagataac ttacttccta aaaaagagga
    54841 tgttgaaaaa agttttaaag aattaaaaga cgctatgggt aaagatgaaa aatataaaga
    54901 acaacttaag aaattaggta tagatgatga gtttttaaga gaccaacaag agaaagattt
    54961 agcaatgcaa aactatcaaa gtaactttgc taaaaagact aaaatatcag atgaagaaat
    55021 gaaaaaatac tatgatacac ataaagatga atttaaaaaa gatgaggtag aagcttctca
    55081 tattttattg aaaacagtag atgataataa caagccactt tcagataagg aaaaagctga
    55141 agctaagaaa aaggctgaag aggctcttaa agaagttaaa tcaggtgagg attttgcaaa
    55201 agtagcaaaa aaatattcac aagatacttc agcaagtgat ggtggtaaat taggattctt
    55261 ttcaaggggg caaatggttg ctgaatttga agatgctgct ttttctatga aaaagggtga
    55321 agtatctgat ttagttgaaa ctcaatatgg ataccacata attaaagtga cagacagaat
    55381 aaatgagcaa acatcatttg aagatgctaa agaaactata aaagaccaac tgttgaaaaa
    55441 taaatatcaa gaacaaatag aaaaattaac taaagaagct aaggtagaaa aagatgaaaa
    55501 agtaataaat aaaatcacaa tctaagactt aaaaaaagag taatatgctt aaaaaagcta
    55561 tatctggata agatatagct ttttttgtat atttttctta acaatggtaa aaataaactt
    55621 aaagtaattt atggagggat taaaaaatat gaaagcaaca ggtatagtta gaagaataga
    55681 tgatcttgga agggtagtta tcccaaaaga gataagaaag acattaagaa ttagagaagg
    55741 agacccttta gagatattca cagcaaaaga tggagaagtc atacttaaaa aatactctcc
    55801 aataggtgaa ttaaatgaat tttcgcaaga atatgctgaa acattagctg aagtactagg
    55861 atatggagtg ttgataacag acttagattc tataatagct gtgtctaagc ttcctaaaaa
    55921 agattataaa gaaaaaagta taagtgatga gttagaagca ataataagtg aaagaacagt
    55981 aaaatattca aaagataaaa acattttacc attattcaag gaagaccaaa aagaatatac
    56041 tagtcaagtt atcatgccta taattagtgc atctggtgac tgtataggtt caattgtagt
    56101 tgtttcaaag gataaagata caattgcaga aagtgaagaa aaatctttaa aaattgctgc
    56161 taacttttta ggaaaacaag ttcaataatt ttatcataaa gaccttttac atgaaggtct
    56221 tttgaagttt ttgtgtaaaa tattgaatat agcattaaaa atatggtata ttaaagtttg
    56281 ttatataaac agtatggagg ttatgttatg aacaataaca aaaataaaga ctcctttcta
    56341 aaaggagccc ttatacttgg gctagcaggt gtaatcgtaa aaataatggg tggatttttt
    56401 aggattcccc ttggaaacat gattggttca gaaggaatgg gatactatca ggctgcttat
    56461 cctgtgtata ctctattttt aacattagct actgctggat ttccaacagc tgtggcaaaa
    56521 ttggtttcgg aaaaagttgc aattggaaat tttaaaggtg caaataagat atttaaggta
    56581 tcacatacag ttttattcat taccggtata atatcatttt gtatattatt ttttggagcc
    56641 gactacatag taacaaacgt aatgaaaaat ccaggtgcgt tatactctat gaaagccatt
    56701 gctccagcat tactatttgt tcctgcaatg tctgcatata gaggatattt tcaagggaga
    56761 caagatatga ctaagattgc tgtgtctcaa gttgcagagc aattttttag agttgtatta
    56821 ggtcttactt tggcctactt gcttatgaag agtctaggtc agacttttgg tgcagcaggt
    56881 gctatatcag gggctacaat aggttcaata gcatctatgc tttatttggt gtttgcttat
    56941 atgctaggga gaaaagagcg tagagcagag atagatgcaa gccaaagatt taaagatgaa
    57001 agagtgtctt atatttttaa aaagctttta actgttgcca ttcctataac aataggagca
    57061 tcagtaatgc cacttgtaaa tatgatagat aatgttattg taataagaag acttatggaa
    57121 gcaggattta catacaaagt ggcaaattca atgtttggtc aattaacagg tatggcaatg
    57181 gccacaataa atttaccagc agtcataaca actgcaatga gtatgagctt agttccagca
    57241 atttctaaag catatgcatt aggaaataag tcaaaagcta gaaaagatac taagagtgca
    57301 gttaaggtaa ctttacttat agtattgcct tgtgcgtttg gtatggcttc tttagcaata
    57361 ccaataatgg gtcttttatt tccacatgaa ccatcttctg taggaactat tttgtttact
    57421 cttactccat gtgtattatt tttaggatta atacagacat tgactggtat aattcaggga
    57481 atgggaaaac caatagtacc tgtaattgct ttatgtgttg gtatgctatg taaaattgta
    57541 ataagttata ctttaacagg tataccagat attaatgtac tgggttcagc atttggtaca
    57601 gttacagcat actttgtggc agccatgatt aacttactat atgttaaaaa acacatgaat
    57661 gtaaactttt ctaaaaaaga atttataata aagccattta taacagttat gacaatgttt
    57721 ataatggtta agttgagtta tggtgcttta gttggattct taggaaacag catatctaca
    57781 ataattgcaa tatgtgttgg tggaattgta tatgtagtag taatacttgg aataggtgga
    57841 attaaaaaag aagagattct aactatgcca aaaggtgata aattatataa attgttaaaa
    57901 aaagttaaac ttataagata atataggaat atatatgcaa ataaaggaaa tactatacaa
    57961 atagtaaatc tccttatttg tataataata taaaaataaa gtgaaatttg ctaataggag
    58021 tgatgttatg ggtaagataa gtattgtagg tcttggacca ggagactatt ctcttataag
    58081 ccaaggtgtg ttagaatctt taataaatag ccctaaagtt ttttttagaa cagagaaaca
    58141 tccaataatt gataaactta aagaaactat acaatacact tctttagact acttttatga
    58201 aaaagaggaa aattttgata atgtttattt gaaaatatct gaatttatca tagaagaatc
    58261 agcaaagggt gacctagtgt atgctgttcc tggtcatcca agagttgcag agaagactgt
    58321 aagtataata gagagattag ccaatgaaaa gcacatagca gtagaaatat tgccttctat
    58381 gagctttgta gacgctatgt ttaactattt ggctatagac ccatctgagg gatttaaact
    58441 ggtagatgct tttgaaatag aaaattcata tatagacttg gatacaagca tgataataac
    58501 tcaggtatat gataaattta tagcatctaa tgtaaagtta aaacttatgg aacattatga
    58561 ctataatcaa gaagtatgta tagtaaatgg agcaggtgtc aaagatttag aaagtaaaaa
    58621 gtttataaag ctttgtgaat tagacagaaa tgaaaacttt tttgactatt taactagctt
    58681 atatataccc aaaagttcta aaaagatgta taatacagta catgacttag aaataatagt
    58741 taatacactt agaagtccat ctggatgtga atgggataaa aaacaaacac atcaatcatt
    58801 aaaaaattct gttatagagg aagcatatga actttgtaat gcaattgata ataatgatat
    58861 agatgaaatg gtagaagagt taggtgatgt tcttttacag gttatattcc attgtcagat
    58921 aggaaatgag gagggctact ttgatttaaa agaagtagta aatggcatat gtaaaaaatt
    58981 aattcataga catcctcata tatttagtgg tgcaaaatta gatatgtcta agtttgagaa
    59041 aacttgggaa gaattaaaga aagaagagaa aggcgaaagt actataacag atggccttag
    59101 aaggatacca aaacatttac cagctcttat gaaggctagt aaagttcaac ataaagcagc
    59161 acttgtcggc tttgattggg acaatgtaga agatgtattt aaaaaaattg aagaagaata
    59221 tcaggaatta cttgacgaac acagggtcgg gaatataaaa tacataaagg aagagctagg
    59281 cgatttatta ttttcgatag taaatctagc tagattttta gacatagact caggagaagc
    59341 cttgaactgt actacagaaa aattcataaa tagatttgac tttatagaaa aaagtgcaat
    59401 gaacttgaat aaaaaattag aagacatgac acttgaagag atggatgagt tttggaatca
    59461 ggctaaaaaa caataaaatt gtggaaggaa atttgtcaaa aatctagaac ttttatcaca
    59521 aagatagaag tctatattat aaacattttt aggaggtaaa tatcgtgaat aaagctgaat
    59581 tagtatcaaa gatggcagaa aaaagtggat taacaaagaa ggaagcagaa gctgcgttaa
    59641 acgcatttat gagttctgtt caagatgcac tagtaaataa tgaaaaagtt caattagttg
    59701 gatttggaac atttgagaca agagaaagag ctgctagaca aggaagaaat ccaagagatc
    59761 cagagcaagt tatagatata ccagcttcta aagcaccagt tttcaaagct ggaaaaggat
    59821 taaaggatat aataaatgga taattagaaa tcttgtgaaa aagtgtggat aaatttgcca
    59881 cactttttct atagaaccaa ggaaggtgat aattatgaga ttagataaat ttttaaaagt
    59941 atctaggatt ataaaaagaa gaactgttgc taaagatgca tgtgataaag ggattgtcac
    60001 tataaatgga aaagttgcta aatcttcttc agaggtgaat attggagata cattagaaat
    60061 acaatttgga gagaagaaga tgaagtttaa gataaatgaa gtaaaagagc atgtattaaa
    60121 aaatgatgca aaagaaatgt acgaaatttt agaataaaat taatagtcct ctagtatatt
    60181 aatagtagta ttattgaaat atttactggg gggatattat ggaacagaac ataactttga
    60241 aagatagatc aaagctggtt atttcgggag tcgagcacat atactctttt aatgacaaaa
    60301 gagtagagct caaaacttct gtaggcgaga tggtaattga aggcgaaaac ctagatatga
    60361 gcaagctaag tatagacgaa aatataatta gcatagatgg aactataaat tctatggtat
    60421 atgcaaagcc taaaaagcca caagagagtt tcttgaaaaa ggtatttaga tagatgatac
    60481 cttttactca agatgtaagc attttttatg ctactattta tggtggaata ctcatagggg
    60541 tgttgtttga tttttataga gggctaagag ggaattttaa attcatcaat tattttgcta
    60601 taatctttga tgtattgttt tggtttctag ctacggttat aatattcgta acaataaacc
    60661 ttacagaatt ttttgattta agatattatc attttgtggc tttgtttatt ggttttattt
    60721 tatactataa caccataagt aaaattgttt tatcaattat caataaaata attcgttttg
    60781 tgagaaattc ttttaaaaaa gttacacatt acatagtcag ttttttaaat aatttgtatt
    60841 acgttataat ctattcacta cacctactat ttgatattat attttatata ccaaacatat
    60901 ttatagcaac aagaaaaagt ataaaaagaa aatcaaataa aaaattaaag aacaagaaaa
    60961 aatcaaaacc taagaagaaa aaaaataaaa ctaaaaaaag ggtgtaggaa aaatgaactt
    61021 aagaaaaaaa ttttcaggac agattattgt tatcagttta tttttaggaa tatcaatttt
    61081 tagtatgatg acaggatttg tatttgagta tacaaaagct aaggaatata aaaaagaaat
    61141 agcaagtcta aataaacaat taaaaaagac tgaaatacaa ataaacagct taaagaaaga
    61201 tgaaaaatca tatgaaggag acttagagga tatagcaaga aaaagattga atatggtaaa
    61261 accaaatgaa acagtttatg tagacataaa tcgttgatta aaacttgcta ataagtttac
    61321 agatgtattg attttaaaat ttaagattta ataaaaaagt cattcagtta aggatggctt
    61381 tttgttttta ttgaggaggg aatttcataa tgaaagtagg agctatagat attgggacaa
    61441 actcaatgag attactgata gcagattata gagatgggaa ttttataaac agaaaaaaat
    61501 ttgttaatac aactagaatc ggttatggtg tggatagtaa aggatatata aaagaagatg
    61561 ctatcaaaag aaatataaaa gcattaaaag aattttctga taagtgtaat gatgaaaagt
    61621 gtgaaaaaat ttattgtatg gggacatctg ctcttagaga cagtaaaaat ggagattact
    61681 ttgttaaatt agctaaagaa gaaactggaa tagatgttga tatagttacg ggaatagaag
    61741 aatcaaattt gggctttaag ggtgttttag aaggaataca aggtgatgat actattattg
    61801 ttatagatat tggaggaggt tcaacagaat ttattgtagg tgattcagaa ggaattaaat
    61861 ttaataaaag tgagaatgta ggtgctctta ggatgacaga gaggtttttg aagcacgata
    61921 ttattgaaga tgaagagttt gaagctatga gttcatttat atatgatgag attgacaaaa
    61981 cgttaaatca tataaaagac agaggattaa aaactcttat aggtatagga ggtacgatta
    62041 catcattatc agcaatgaat caagaattag agatttactc tatggaaaaa atacataata
    62101 gtaaaataca caaaaaagaa atagaagaaa ttctacaaaa attaataaag atgacattaa
    62161 gtgataaaaa gaacttaaaa gggctacaag aaaaacgtgc ggatattata actgctggtg
    62221 taaaaatatt gaatataata atggaaaaat tagaaataga agaaataacc gtaagtgaat
    62281 acgataattt ggaaggctta atatgtcaaa agtcaaaaaa aatgtattaa tattttttga
    62341 tttttaaaac cgattatgac aaaaaaaatt actcctcttt gttacaattc ttaatataaa
    62401 caaaagagga ggttttttta tgcaaagaag tgtagcagtt gtgaagaata agactgtaaa
    62461 accaaatgtc ataaaattaa taagcggata tattgatttt actacggcgg cattcgctgt
    62521 gatgggattt ttacttagta ggtctattat agtagactct gtagctcctt taggaattgc
    62581 cttttttata tgtgcatcaa agattgataa gtacaagata cctgtattca tatcaacatt
    62641 attaggaata ttgttatcat ctaatgattt tggaaatgta gcaaaatatg ctgtgtgcct
    62701 tacaatattt atggctatga ataaaaaatt aaaattatca gattcaattg ctaaggttgc
    62761 atttataggt gcaataatac tacttccaat ctccatggga caagctttct tttcaaatag
    62821 gtatgtatat ggtcttctta tagcaggagt agaagcaata ataatgttta tatctatata
    62881 tgtgttttct tatgggatag gtttaatggc caatgtcaac aataggatgt ctgtacggac
    62941 agaagaagct atatcaataa gtttactgct aacctttagc ataatgggaa taggagatat
    63001 atctctgttt ggaatatcaa taaggaccat tttatcgacc gtattgatat tgctgacctc
    63061 aatagtaggt ggagctacca tgggagcatc tagtggtgta attattggtg tagcatccat
    63121 tttaaataat atgacatcag ctgtttatat ggggatatat tctttttcag gattaataag
    63181 tggagcattt aataaaataa ataaatattt ctgtatacta ggatatattc taagttggac
    63241 aataatatac ctatatactt ctggtattac ttctaatatg atgcaattaa gagatatact
    63301 attaggttgt ttgatagtac tagtattgcc agagagatta tttaataaga ttgaaaaatt
    63361 aataaaaagt aatgtggctt caaatgaaat cgtatacgat tatattatga gaagcaaaaa
    63421 cctaactaat agcagattga acagtattta taaaacttat gatgaccttg cagatacatt
    63481 tgataagata agagaaaaag acaaagtgct agaccaaaga gatatagcta atgtaataga
    63541 tatgattcat aatgatgaat gtaaaagttg tagtatgaga agaatgtgct gggagtcaag
    63601 atttaatcat acatacacta tgatatataa cattcttgaa aaaattgagg agaaagggga
    63661 gttaagtcta aacgatatac ctaaaaattt tagaaaggaa tgtatgaaac cagagtcgat
    63721 agtgaaaata tcaaatcact attacaaaat gtttgtatta gattatgatt ggagtgtgaa
    63781 gttttcagaa agtagaaagt taatagcaaa tcagataaga agtatttcaa aatccattaa
    63841 atccttatct caagatttag aaggtgacat aatgctagat atagaaaagg aaaaaaatat
    63901 atatgaacag ttagaaagat atgatataac agttgataaa gtaagttact taacaaaaag
    63961 taacaatgag tttgaaatat caatagaaaa gaaaacttgt catgatggat gtatgtgtga
    64021 agataaaatt gtaaatataa tttcagacct agtaggtgaa aatatgtctg ttagaaaaat
    64081 aggatgtcac tgtttaggag gtaaatgcaa ggcaacattc gttaaatctc aaaaatataa
    64141 ggctgttaca gaagtttcag caatgtcaag ggatgggcat atcctttgtg gagacaatta
    64201 cacatacatg gagataaatg atggtaaata catgatggct ataagtgatg gtatgggaaa
    64261 gggtaaaaaa gcatatgaag aatcttctgc cactattgat atactggaaa aaatgataga
    64321 tgcaaaaatt aaagatgaga tagtcattga tactataaat aatatgttgt tgttaaaatc
    64381 ttcagaagag atgttttcaa ctctggattt gggaatttta gatttaaaac gaggttgttt
    64441 agaaactata aagatgggag cgtgttctac ttatataaaa agagaggatg gagaagttga
    64501 cttaatatca tcatcatcat tacctgtggg tatattatca gatgtaaaaa ttgatagaaa
    64561 aaatgtaaaa gttaaggaag gagactatgt tataatggtt tctgatggaa ttgtagatgc
    64621 aggaagaaat aacaatttag gagacaattg gttaatatac ttcttaaaga atatagagac
    64681 tacaaatcca aaggaaattt caaatttaat attggataga gcattagagt tacaagcgtt
    64741 acaaatcgaa gatgacatga ctgttttagt tacgaaaata tgtacaaaat aaggtttaca
    64801 agattatgtg tataagatgt ggataacttg tgtataatat gttaatagac ttttgaaaaa
    64861 agtatagtta ttaacaaaaa taacatataa atcaacaaaa tttgttgata agtatggcag
    64921 aaatgttaat aaaatgtaaa aaaagaaaaa acaaaagcta ttatcaaaag agattcatgt
    64981 tgaaataact aaatttctaa gtaaagaaaa cctgtgaata tgttgattat tatgtggata
    65041 actattaatt cgatatttat ttatatagta tgaataattg aataatatat ctaaaaaaag
    65101 ccatcctatt attaagtaaa tttattgaaa ggatggcttt ttatgaatag aaaattgtgc
    65161 aaagcaataa tacctttatt tatttctatt ttttgcatgt taataataag tataaaagct
    65221 gaccaaggat atcaaaagaa taatgacaga gcaattgctg tcatgaataa taatgatact
    65281 gaagatgatg ctgaaaaaaa acaagaagta gactatgaaa aaacatgttg gaatttgcaa
    65341 tctttgttca aaagtgatga ccaatggaaa aaagaactta agagttttga taaagatact
    65401 aaagagttga aaaattacat aggtaaagta acaaaatcgc caacacattt gtcttttgcc
    65461 ttaaacatta aggaaaaact tgatataaga ctaaataaac tttctgccta tgtaaaatta
    65521 aaacaagata ctaataaaaa ttcatataag tacttagata tgagtgatag tatgagtaaa
    65581 tcatatggga attatatggg gatttgttca gatttagaat tagaaatact aaaattatca
    65641 aacaaagatt acaagaaaat tatttcaaat aaaaatataa ataggaaata tggtgcatat
    65701 ttaagagata taagaaggaa taaagtacat tatttggatg ataaatcaga agatattcta
    65761 agcaaagtat cttcaataag tagtcttcca agccaagtat atgaattatt tagaaatatg
    65821 gatagaaaaa ctaatttgac accagcgcaa tatgcaagtg aattagagtc agtagataga
    65881 gaaaatagaa aaaaagctta tcaagatgaa cttataactt acaatgataa tataaataca
    65941 attgcaggtt taattactgg tcaagttaaa aagaatattt tttattctca ggaaagaggt
    66001 tatgagtctt ctttagaaat gtacttggaa tctgataatg tagatgaaaa agtatacaat
    66061 aatttgataa ggactgtaaa tagtaatatg gacagtctac acaaatatat agatttaaga
    66121 aaaaaagtat taaaattaga taaggttcat tcatatgata tgtttgtgcc tatggttaag
    66181 gctgttgata ataatattaa ttatgaaaag gcacaaagta tagtatattc agcgttgtca
    66241 cctttaggaa aagaatacaa tgatgtagta tataaagctt tcaatgaaaa atggatagat
    66301 gtttattcac atgaaaataa agtaagtggt ggatactgct tatctgttta tgaaaatcat
    66361 ccatatatac ttttaaatta taataattca ttaggttcag tttcgacttt atcacatgag
    66421 cttggacatg ctgtatatga atacttaagt tctaagaatc agaattattt taattcaaat
    66481 ccgtctatat ttactcacga agttgcatct acaacgaatg aagcattact ttacgagatg
    66541 cttataaaag aagctaaagg caatgatgaa aaagcttatt atataacaca gtatttagac
    66601 ttgataaaag atactcttta tacacaaact atgtatgctg aatttgaaaa aactatacac
    66661 gagttagtag aaaaaggaaa aagtgtaaac gcattagtat taaatgatgt ttggggacaa
    66721 cttcttaaaa aatattatgg acaaagctat gaattagacc aattatctaa ggtcggatgg
    66781 gctagaatcc cacattttta taatagtttc tatgtctata aatatgctac aggttgtagt
    66841 gcaggagtaa gttttgccca agacatccta aagaatggtt cagataacta tatgagcttt
    66901 cttaaaaagg gaagttcaga ttatccaata aacttgttaa aatcaagtgg cataaattta
    66961 actaatacaa aacccataga aaatacaatc aaaaaatttg atagtttagt agcagaactt
    67021 gaaaagctat tataaacatt attgtaatcc acatggtaaa tatatcgaat taatgataaa
    67081 atatactaaa agtttaaagt cctatcaaga taggactttt tatttattaa ttagtgggag
    67141 atgattctat gcaagttaaa gttaaaaagg ctgtaatacc agcagcaggt ttaggaacta
    67201 gatttttgcc agctacaaag gcacaaccaa aagagatgtt accaatagtt gataagccaa
    67261 ctctacaata cataatagaa gaagctgtgg cctcaggaat agaggaaata ctaataataa
    67321 caggtagaaa taaaaaatct attgaagacc actttgataa atctgtagaa ttggaacttg
    67381 atttagagaa aaaaggtaag aaagaattgt tggaggtagt acaaaacata tctaacatga
    67441 ttaacataca ttatataaga caaaaagagc caaaaggatt gggagatgca atttattgtg
    67501 cgaggcattt tataggtgat gagccgtttg cagtaatgtt aggggatgat attgtagata
    67561 atgatgtacc ttgtctaaaa caactaacag atgcatatga ggaatataga actacaatat
    67621 tgggtgttca aaaggtaaat caagaagata ctaataaata tgggataata gaagcaaaaa
    67681 atatagaagg tagagtttac aaggtaaaag acatggtaga aaagccagag tcaggaaaag
    67741 ctccttcaaa tattgctatt ttaggtagat atataataac tcctgaaatc tttgatattc
    67801 ttaaggattt accaccaggt aaaggtggag aggtacaact tacagatgct ttaaaaatat
    67861 tatctaagaa agaagctatg tatgcataca attttgaagg caagagatat gatgttgggg
    67921 acaagctagg atttttagaa gctactgttg actttgctct aaagaaagaa gaccttaagg
    67981 aagattttat aaaatatttg aaacatgtat gtagtgaatt tgataaaaat aacaatgtgt
    68041 tatacaataa tacagatgaa aaaatagatt taattgaagt caatgaagct agagaacttg
    68101 aaattcaaaa ataattaatg aaaggtgatg tatggtggca aaatcaagtc agaaaagaat
    68161 taatagaata atagctgttg ggattgtggg aatcctcgta ataacgagta taacagctgt
    68221 tttgagtatg attgcaacta tgtaataaga taaatgacag cagttgatat acataaatat
    68281 aaaaaccata tataacttga tgtagtcaaa aacttacaat attttagtta tatatggttt
    68341 ttagaattta taaatgtaaa aaaaaatttt tataatagtt gcatatttta aaggaaaata
    68401 ttattataag atataaatat agtgataaat ttataaaaac gtgctaagat tggacttgaa
    68461 atacagtaat atagtaggta ataatgtttt aaattttatt tattgaccat aatatataaa
    68521 taatgatatt attagactta actgtttaaa taaaactttt ctttttcatg ccttcaattt
    68581 ccaaaataag tatgtatata aatgaatata tagatgagga ggattttttt gattttagat
    68641 gataaagtat tagaaggatt aggtagtaag accttagttg agctacgaga gatagctaaa
    68701 gaattaaaaa taaaatcaat tactacatat aagaaaaatg agttaattga gattataaat
    68761 tccaaaagta aaaatgagga aaaagtaaac gaagagacta aagaaaaaaa tgaagatatt
    68821 aaagaagaca taaagaatag aaaagaaaat atagagcgtg aaagtataga aaaagaagtt
    68881 gaatataaga gccctaccaa atcatacaac aagaaagaaa tagataccca aaatatttcc
    68941 caaaaccaag aaaatagaaa tcaaagaaat gactataata agacaacgag tgtaaatgat
    69001 agtaataagt catcaaatat atctaataga atggtcagaa ataataataa aaactattat
    69061 atgcctaaac aagttgatga atctaagata gtagatgaat ttaacacatc aaaagaggat
    69121 gaagtagtag gtgtacttga aattctgcca gatggatttg gttttttaag aggttcaaat
    69181 tatttgtcta ctgaaggaga tgtttatgtt tcaccatctc aaataagaag atttaatatg
    69241 aaaacaggag acaagattaa gggaataact agacatccaa aaagtggaga gaaatttaga
    69301 gcacttttat atgttcaaaa aataaatgat gaaaaccctg acacagctat acaaagaaat
    69361 gcgtttgaga cattaacacc aatatttcca gaagaacgac ttacactgga aactaataga
    69421 aatgaaatag ctacaagaat tatagattta atttcaccta taggaaaggg acaaagagga
    69481 ttgatagtag cacctccaaa ggctgggaaa acagttcttt taaaaagcgt tgcaaatagc
    69541 atagctaaaa atcatccaaa tgttgaactt atagtactcc ttatagatga aagaccagag
    69601 gaagttactg atatgaaaga atctattgaa ggagacgtta tatattcaac ttttgaccaa
    69661 gtatcaagtc atcatgtcaa ggttgcagag atggtgttaa atagagctca aagattagtt
    69721 gaacatggta aagatgtagt aattttacta gatagtataa ctagattggc aagggcatat
    69781 aaccttacaa tatctcctac aggtagaact ttatctggag gtattgaccc tggtgcactt
    69841 catggaccta aaaagttctt tggtgcagca aggaatatta gacagggtgg ttctttgaca
    69901 atattaggaa cagcattggt tgaaactggt tctaggatgg atgatgtaat atttgaggaa
    69961 tttaaaggta caggaaatat ggagctacat ttagatagaa aattagcaga aaaaagaata
    70021 ttccctgcaa tagatattta caagtctggt acaagaagag atgatttatt gcttgatgat
    70081 gaagaaaaga ctgctctttg gagattaaga agagaaatga gtaataattc agtaatggaa
    70141 attacagata aagtcattga acttataaag agaactaagg acaataaaga gtttgtaaaa
    70201 tcaataaaga atttgtaaaa tcaataatac aaatgcaaat atggattata taaactaata
    70261 gaaaaatcac ttgaatggca taataagttg tgctataata atgtagttga taatgtaaaa
    70321 agaaagttaa tataattaat ttttttaaat aaagagaaga ggtgactata atgcaaaagg
    70381 aaatacaacc aaaatacaat ccagttgaag tgcgttgtgc ttgtgggaac acatttgttg
    70441 ctggttcaac taaggacgaa ataaaagttg aaatatgttc agagtgccat ccattctata
    70501 caggaaaaca aaagaacata gaaaaaggtg gaagaatcga caaattcaag aaaagattca
    70561 aaatggacta atttttataa aagtggagtt ggaggaagcc aactcctatt ttattacaaa
    70621 aaaacattca tattctactt taaattgtct ttttgtatta taataataaa tatgtaaaat
    70681 tgagtattct aaatacaata aaatgaggtg agtggatggc aaaacaagct gtaggtgggc
    70741 aagctgttat tgaaggtgta atgatgcagt caaaagacaa aagagcggta gctgttagaa
    70801 aaagtgacgg agaaatagca ctaaaagaag atagaataaa aagttgggta agagataaga
    70861 atatagataa gatacctttt gttagaggtt cttttgttat gatagataca atgattcaag
    70921 ggataaaaag tttaaacttt tcttcagagt tttttatgga agaagctgaa gaggataaat
    70981 ttgatttatt tataaagaaa atttttaaag ataaggcaaa tgatataatt ataatatttt
    71041 cattggtcat agcaatgtta ctttctgctg gattattcat ttttatacct acattggtag
    71101 gaggagcttt ttctaaagta atgcctaatg attttatgct taatttaata gaaggaatca
    71161 taaggatagc tatattattt gcatatatag tattgatttc acgaagtaag gatatagaaa
    71221 gagtctttca atatcatgga gcagaacata aatctatata ttgctatgaa aatgatttag
    71281 aacttacagt agagaatgct agaaaattta aaagattaca tcctagatgt ggtacaaatt
    71341 tcctatttat agttatggct gtatcaataa tattgtttgc attttttggt tggccaaatc
    71401 caattcttag aatatttatg agaattatat gtgtgcctat agtagcaggt ttatcttatg
    71461 aggtaattag agttcttggg aaatatgata atggatttac taaaattatt gcatatccag
    71521 gaatgatgtt gcaatatttt acaacaaaag agcctgatga tgagcaatta gaggttgctt
    71581 tggaagcatt gaaagcagta gtagattaga gaaaaggata tttgttatga cgataaaaga
    71641 tataattatt aaatattcag ataaactaaa agatataagt gatactccaa gattagatac
    71701 agagttattg ttacaaaaaa cattgggtgt tgatagatta tatattcact taaatctgaa
    71761 taaagagcta actgaagaac aaaaaacaaa atttatgggt tttgcagaag aaagattaaa
    71821 tggaagacca atagcatata tagttgaaaa tagggagttt atgggattag atttttttgt
    71881 taaggaaggc gtattgattc caagaccaga tacagaaact ttagtagaag aaataattga
    71941 aatatgtaga gaaaaaaaag atgtaagtat attagatata ggaactggtt ctggagctat
    72001 tacaataagt ttagcaaagt acattgaaaa ttctaaaatc atgtcttttg atatatcaga
    72061 gacagcatta gaaatagcta aaaaaaatgc tattataaat gaagttggtg aaaaaataaa
    72121 gtatattaat tctgatttat ttacagcaat aagtgatagt aacataaaat tcgatatcat
    72181 agtttcaaat ccaccatata ttaaaaaaca agatatagaa actctgcaca aacaagttaa
    72241 agattatgaa ccatataatg ctcttgaagg tggagaagat ggattagact tttatagaag
    72301 aataacagaa caaggtaaga aatatttaaa taagtgtgga atattagcat atgaagttgg
    72361 tcataatcaa gctgaagatg taataaatat tatgaaaagt aatggatata aaaaaatata
    72421 cacaaagaag gatattcaag gcattgatag agttgttata ggttacaata tatgatataa
    72481 tcaacaattg atatgttaca tttaatcgag gtgaaagtat gttaaaaaaa ttagaggtat
    72541 tggaagatac atataaagac ttaagtgaaa aaataggtga tccggatgta ataaatgatc
    72601 aaaaagtttg gcagaaatat ataaaagaac atgctgactt ggaacctatt gttatgaaat
    72661 acagagagta taaaagcgtt ttagatagta taaaagaatc aaaagaaatt ttacaagaag
    72721 agtcagatga agaattaaga gaattagcta aaatggagtt agctgagatg gaagaaaaag
    72781 tagcacctct ggaggaagaa ataaaaatac tattattgcc taaagaccct aatgatgata
    72841 agaacgttat agttgaaatt agaggtggag ctggaggaga tgaagcagct ctatttgcgg
    72901 gtgatttatt tagaatgtac tcaaggtatg ctgaaagaag aagatggaaa atagaattat
    72961 tgagtgcaag tgatactgga gttggtggat ataaagaagt atcttttatg ataaaaggta
    73021 aaggtgctta ttcaagatta aagtatgaat ctggagttca tagagttcaa agaataccat
    73081 caactgaatc tggtggaaga atacacacat caacttctac tgttgcagta ttaccagaag
    73141 ttgaagatgt agaggttgaa ataaatccaa atgatttaag aatagatgtt ttccgttctt
    73201 cgggtaatgg aggacaaagt gtcaatacta ctgactctgc tgttagggta acacatatac
    73261 ctacaggtga agtagtatct tgtcaagatg gaaaatcaca attaaaaaat aaagagcaag
    73321 ctttaaaaat attaaaagct agactttacg ataaagcatt ggcagaacaa cacaaagata
    73381 tagcagcaga aagaaaaagc caagtcggaa caggagacag gtcagaaaga ataagaacat
    73441 ataatttccc tcaaggtaga ataagtgacc atagaataaa tttaacttta tataagttag
    73501 atgcattttt agatggagat atagatgaaa tgatagatgc tttaattact gttgaccaaa
    73561 cagaaaaaat gactgcaata taagtaatgt tttaaagaag ctgtttcttc ctagaaatta
    73621 ttttctattt tgaaacagct attttgcatt ttaggtcatg ttatcatatt ctattgtata
    73681 taattgttat atacaagtat aggagggtta tatgatacta aaagtaacaa taattgggct
    73741 tttggcaggt gttataggga caggattagg aggagtaata tcagccattt ttaaaagaga
    73801 ggtcgataaa tatctaagtt tttttatggg attatctggt ggaataatgt tggctgtagt
    73861 ggtgtttgat ttaatgaaag aatccatgga caaaatgggg ataatcaata cagttatatt
    73921 tacgtttgtt ggagctctca taactatgta cataaaaaca aaattagatg tgtctggaaa
    73981 tatggcatct ggatatctta tattcataag tatattacta cataatttac cagaaggact
    74041 agccattggt tcttctttta tgtcaactga aagtttaggt attacacttg ctatagttat
    74101 aggtctacat aatataccag aagggttagc tatggcatta ggcttagttt gcaataaaat
    74161 gaaactaagt aaagttattt tgtttactgt aatagctgga ttacctatgg gtctaggaag
    74221 ttttttaggt gtttattttg gaggagtatt tacttctctt ataggagtat ttcttgctac
    74281 agcaggaggt actatgatgt atgttgtatt ggaagaaatt tttccacatt caaaaagtgt
    74341 gtattgtata attggtttct tgttaggtac aatgattgta aattatatat aaaattaaaa
    74401 tgaaagcagg catacaaaag atgaaaacta ttataagtaa tattgatata aataatattg
    74461 attatgagga aataaaaata caagcaaaac ttcttagaga aggtaaaaca gttatttttc
    74521 caactgagac agtatatggg ttgggtgcaa atgcattaga tgaaaatgca gtaaagaaga
    74581 tttatgaagc taaaggaaga cctagtgaca atccattgat agtccatata tatgaaaaag
    74641 aagaagtata tgacttagcc aaagatataa gtgataaagc taaacttgtt atagaaaagc
    74701 tttggccagg acctataaca attatattaa ataaaaaaga tataattcca tacaaaacta
    74761 gtggagggct tgatacagtt gcaataagga tgccttctaa tgttattgcc agggcaataa
    74821 taaaagaagc tggtatacct attgcagctc cttctgcaaa tatctcaggt cgcccatctc
    74881 caacaaaagc aaaacatgta tatgaagaga tgaatggaag agtggatgga atagttttag
    74941 gaggagacag taattttgga ttggagtcaa ctgtattgga tttaacagaa gagactccta
    75001 tgatactgag accaggaagt ataactaaag aagttttaga ggaacttctt ggagaagtaa
    75061 agcttgaccc atcattaagt aaaaaagaag ataatcaaaa agctaaagct cctggaatga
    75121 aatataaaca ttattctcca aatgcagatg tatatataat atctggaaaa agagaaaatg
    75181 tagcaacaaa gattaatgat atgataaaat ctaatagaga caaaggtcta aagacaggtg
    75241 taatgtgtat cagtagaaac cgagagtttt atgatggaga agtaatcgat ttaggaaact
    75301 ctcttgaaga agttgcaagt aatctgtttg atgctcttat agaaatggac aaaaagggcg
    75361 ttgatgtcat atattcagaa gagttcccta agactggagt tggacaagca attatgaata
    75421 gactactgaa gtcagcagga tacaaagtga taaaggctta agagggagtt gtaaaatatg
    75481 aatatactta ttgtgtgtac aggtaatact tgtagaagcc ctatggctga ggctatacta
    75541 aggaaagcaa ttaaagaatc tggaagaagt attgaagaat attcgatatc atcagcaggt
    75601 atatctacag caaatggaat gggtgcttct gaaaattcta tagaagtact aaaagaaatc
    75661 ggtatagact tatcaaatca tagaagtaaa gtaattacta aaaaattaat tgatgaatca
    75721 gatataatac taacaatgac taaatctcat aaagaaattt tagtacaagc agttccaaaa
    75781 tgcaaagaaa aagtgtatac ttttaaaggg tttgcaaata aaaatgagga agatatatct
    75841 gacccatttg gtggaaattt ggatatttat agaagcacta tgagagaaat aatgtattct
    75901 gtcaacgaaa tagtaaaaaa aatatgaaac ggaggataaa caaatgaaga taggattagg
    75961 ctgtgaccat ggaggatata atttaaaaaa agaaataata agctatttag aaagtaaagg
    76021 tatagaatgt gtagattatg gaactaataa tgctacagat tctgttgatt atcctgttta
    76081 tggagaaata gttgcaaatt cagttataaa taaagaagta gattatggaa tattatgttg
    76141 tggtacaggg ataggaatat ctttagctgc taataaggtt cctgggataa gatgtgcagt
    76201 tgtatcagat gtattttctg caaaaatgtc aaaagcacat aatgatgcaa atatgttatc
    76261 attaggcgaa agagttttag gaaaaggtct agcacttgag atagttgaag catggattaa
    76321 tacggatttt gaaggtgata gacatgctag aagagtaaac atgattaaat caatagaaga
    76381 aaaacataat aaataataaa aattacagtt ttatcaggag gaattatcaa aatgagcaaa
    76441 gtggtagaga caaaccatcc attaatacaa cataaattaa ctttaatgag agataaaaat
    76501 actggttcta aagactttag agagctttta actgaaattg caatgttaat ggggtatgaa
    76561 ataacaaaag atataccatt aaaagatgta gaaatagaaa ctcctattca aaaaacatca
    76621 tctaaagtag ttgcaggtaa aaaattagca ataattccaa ttttaagagc aggtcttgga
    76681 atggtagatg gattagtaag cttaatgcca gcagcaaaag ttggacatgt aggactatat
    76741 agagacccag aaacattaaa accagttgaa tattattgca agcttcctca agacatagga
    76801 gaaagagata taatagtagt tgaccctatg cttgcaacag ggggttctgc tgtagcagct
    76861 atagatttac ttaagtctaa aggagctaaa agtataaaat tagctaattt agtagctgca
    76921 ccagaaggta tagcagaagt gcaaaaatat catgatgatg ttgatatata tgttgcatca
    76981 gttgacgaaa gattaaatga acatggatat ataattcctg gtcttggtga tgctggagat
    77041 agattgtttg gaactaaata aaaataaata actataaata tttattaggg actttcttat
    77101 atagtgtata taattttatg taagaaggtt cttttttata tattttttct caaaatagga
    77161 attatgaatt gataaatttc tcgaattgag aataaaaagt ttttaccaat taaaaaagat
    77221 tgcttatttt acaaggtttt atagaatagt agtctatttt aatgtggcat gttagttgct
    77281 tacgattaag attataggta ttaagtgtat tagttaaatt gagaattgaa tattaataat
    77341 attattggga gtggtaatac atgaagaatt gtatagcagt aagaggggct ggagatttag
    77401 caacagcagt tattcataaa ttacacaaca gtggttttaa agtagttgcg ttagaaatag
    77461 aagagccatt agctataaga aggcaaattt ctttttgtga ggctgtttat gaaaaaaaag
    77521 taactgttga aggtgtagaa tgtatatttt gcaatactga aaaagaaata gaagaagttt
    77581 tatctcaaca taaagtagcc ttgattatag acccaaaagg agaaaaatta tctaagttaa
    77641 atccaatcgt tttggttgat ggaatactcg ctaagaaaaa cttaggtaca actaaagata
    77701 tggcaccact tacaatagct ttaggtcctg gattttgtgc aggagaagat gtagatgttg
    77761 ttatagagac aatgagaggt cataatctag gaagaataat aactaatggt agagcagaaa
    77821 aaaatacagg agtaccagga gcaataaaag gtttttctaa agagagagtt atgcattcac
    77881 agtatgatgg tataatggaa aatgtatgta agataggtga tatagttgaa aagggagacc
    77941 tactttgtta tattaataat tatgaaaaaa aacatgaagt tagagcaaca atatctggag
    78001 tccttagagg tttattaaag gatggagcaa aggtgaacaa aaatttaaaa atactagaca
    78061 tagaccctag aaaagaagaa gttaagaatg cttttacaat atcagataaa gctagatgta
    78121 tagctggagg ggttttagaa gccatactaa acaatgggat ttatccccaa aattaattta
    78181 tattagataa agctattaat attttattaa gatgatataa taaatttaat agattaaaga
    78241 gagcatatga cgggtattta aaatttaaat ggtaaaaatg ctcttttttt attggacatt
    78301 caattaacat gtgtatataa tataaaaata agtatttatt taggttgaaa tctaataaat
    78361 atataatacc agtacataag caaagaggag aaaagtaaat tatgagacca tcatgggatg
    78421 aatacttcat ggaaattgca gaagtagtga aaaagcgttc aacttgtatt agaagacaag
    78481 taggagctgt aattgtaaga gacaaacaaa tattaacaac tggatataat ggttctccga
    78541 gaaatcttga acactgtgaa aatattggat gtaagaggca ggaattaaat attccctcag
    78601 gagaaaggca tgaattatgc agagcattac atgctgaaca aaatgcgata atacaagctg
    78661 ctcataatgg tattagtgta gatggggcaa ctttgtatgt tactacaaga ccttgtgttt
    78721 tgtgtgcaaa aatgtgcata aatgcaggta tattgaaaat tgtttatgaa ggtgattatc
    78781 cagatgatat gtctacagaa ttattaaaag aagcaggaat agaactcata aaattttagt
    78841 ggataaaatt tatatgacaa taaatgtcaa attatgacac tgaaatacaa tggtatcaaa
    78901 tgattaaaag aaagtatttg aaaaatcatc agattagtag taagatatat tttataatta
    78961 aataaattta tgagatagat taattacatg ttatttattt tataataatg agggatacta
    79021 tttatgagta aaagaaatac acatacagaa attgcaaagt tgtttagttt aataagtcaa
    79081 ataggattga tgattgtaat atcaatatta ggatgtactt tcttaggaaa atttttagat
    79141 agtaaattga atactactcc agttctgaca ataatatttt tattgttggg tgttggaggt
    79201 gcttttagtg gtgtttataa aactctaata gtatatacac gaaggaagtg atatattgga
    79261 tataaaatta cttaaccagg taaggtcagt aacaaaaggt atagtaatat ttgatgtagt
    79321 tttaattgtg ctaatgttga taacatcaac attaagtaaa ccagtattat taggacttat
    79381 atttggctct ataatagcta tgttaaattt tagattattg gcgatatcac tggaaaaatc
    79441 agtgacattt cctgctggga aggcacaagc ttatgcatca gcacaatatg ctattagatt
    79501 atttataatg gcagttgttc taattgcatc agctacagta ccacatttaa atataatcgg
    79561 aacagcattg ggattattga gcacaaagtt tgtcatatta tcaaaaaact ttataaataa
    79621 attaaagagg aaggaggcat aagtatgaat caagcgcatt attatctcat aggaaacttt
    79681 aagattagtg aaacaatact agtaagttgg ggaataatgg cctttttagc gatagcatct
    79741 tattttttga ctagaaactt aaaaaaagtt ccaactagta aagttcaaat attcttagaa
    79801 tttgcagtag gtggtcttgc taaaatggtt acagatgtta tgggtaaaaa tacagtaaaa
    79861 agaatgccat atatgatacc atacatagga agcttgtttt tatttttcgc atgttcaaat
    79921 ttaagcccat tacttggatt aaggtcacca acaaccgact tagacacaac tgctgcatgg
    79981 gcaatgatta cattttttat gatttatttt gcaggagtta aatttaaggg gttatcatac
    80041 tttaaagaat tggctcaacc acttgccata atgttaccaa tcaatattat aggtgagtta
    80101 gctagaccaa tatcattaag tttccgtcca tttgggaata ttttaggtgg aacaataata
    80161 atgggtctgt tttatcaact tttaggtttt atatctagtt taataccagg attatcaata
    80221 ccaattggag aaatattaat accagtccca ttacacttgt actttgactt atttgcagga
    80281 ttgttacaag catatatatt tataatgtta acaatggtat ttatatcaag tgcaggagag
    80341 ttagattaag aaatcaactt atattaagat aaaaataaaa taatttaaaa aattaaagtt
    80401 ttaaggagga aataattatg gaaacagcaa tagtagcggc agcatcagca ataggagcag
    80461 ggatagcagt tgcaacaggt ataggtgcag gtataggtca aggtatagca gcagctaaag
    80521 ctgcagaagc agtaggaaac caaccagaag ctaaaggtga tataacatca actttattac
    80581 taggagttgc tatagcagag tcttcagcaa tatatggttt ggttatatca ataatacttc
    80641 tattcgtaaa tccattcttt aaatacttag gaatgtagat ttagtcctta aatattttga
    80701 gataggttta taggtaaatc atctcaaact tataatcatg agatgaactt tatcatctcg
    80761 tgattataag tagttatgat ttatggaaag gaggataatt atggaaaaag cactagtagg
    80821 tataacgtgg gaatttgtgt tccaaattgt taatactttt ataatatttt tacttctgag
    80881 aaagctttta ttcaagccag tattaaatat aattgaatca agagaaaatg atattaaaag
    80941 tgatttggca gaaggcgaaa aagcaaaaaa tgaaggttta gctctgaaaa aagaatatga
    81001 gtcaaaaatc aattttgcca aggatgaagg tcaagaaatc ataaaacaag caactataag
    81061 agcagagcag aaatcagatg atatagtaaa tactgcaaag aaagatgctc ttgatataaa
    81121 agaaaaagca aacaaagata tagaacaaga aagacaaaag gttataaatg aaatcaaaaa
    81181 tgatatatct aacatagcac tacttgcagc atctaaagtt attgaaaaag atttagataa
    81241 atctaaacat gaagaattga tagaaaactt tataaaagag gtaggggaag ctaaatgata
    81301 aatgtaatag caaatagata tgccgaagcc ctatttcaat taggagaaga agaaaattca
    81361 actgatgtct tattcaaaga actagaaaaa gtagtagata tgatgactaa agttagtaaa
    81421 gatttctaca aagttttaaa atcaccatta gtttcaaaat cagaaaagaa aaacttggta
    81481 gaaattattt ttagtaagga agtaagttct aacataaaaa actttttaaa agttttagta
    81541 gataaagata gaatatcata tttagaagat atcgaattag cttataaaga attattaaat
    81601 aagaaaaaca atgtaattga tggagtagca ataagtgcta ttccaatgag tgaaacagat
    81661 attaaagaac ttgaagtcaa actttcaaat aaatacaaca aaaatgttac tatagaaaat
    81721 gttgttgaca agactatact agggggagtt ctagttagaa ttggaaatga acaaatagat
    81781 ggcactgtga aaactcgtct tgataagatg aaagaaaagc tatctgaagt aatatcttaa
    81841 gaatggcggt gaacccatga acttaaaacc tgaagaaata agttctataa ttaaacagca
    81901 aataaaaaat tatgagaata aagttgagtt gacagataca ggtagtgttt taacagttgg
    81961 ggatggtata gcgagtgtat atggattaga aaaagctatg tctggtgagt tgctagagtt
    82021 cccaggtgaa atatatggta tggcacttaa cttggaagaa gaagtagttg gagctgttat
    82081 ccttggagat gacagtgaaa taaaagaagg cgatatagtt aagagaactg gaagaatagt
    82141 tgaagttcca gttggagaag ctttaatagg tagagttgta aactcactag gtcagccaat
    82201 agatggaaaa ggacctatag cgtacacaaa aactagacca gttgaatctg aggcacctgg
    82261 tataatagat agaaggtctg tttatgaacc attacaaaca ggaatcaaat caatagattc
    82321 aatgatacca attggtagag gtcaaagaga gttaataata ggtgacagac aaactggtaa
    82381 aacatctata gttattgaca ctatactaaa tcaaaaagga aaagatgtta tctgtatata
    82441 tgtagcaatt ggacaaaaac gttctactat agctcaatta gttagctctt tagaaaaagg
    82501 tggagctttg gattatacaa tagtagtttc agcaacagca tctgaatcag caccacttca
    82561 atatatagca ccatatgctg gagcagctat gggtgaagaa tttatgtaca atgggaaaca
    82621 tgtacttata gtgtatgatg atttaagtaa acaagctgtt gcatatagag aaatgtcatt
    82681 gttacttaga agaccaccag gacgtgaggc atatcctgga gatgtattct atttacattc
    82741 aagattactt gaaagagcag ctaagttatc tgatgaattg ggtggaggtt caatgactgc
    82801 cttgcctata atagaaactc aagctggtga cgtttctgca tatataccaa caaatgttat
    82861 atctataaca gatggtcaaa tatacttaca accagagtta ttctattcag gtgtaagacc
    82921 agcagttgac cctggtatat cagtatcaag ggttggtggt tctgcgcaaa ttaaagcaat
    82981 gaaaaaagtt gcaggaacat taaaacttgc atattcacaa tatagagaac ttgcagcatt
    83041 ctcacagttt ggttctgact tagatgaaga tactaaaaag agacttgctc aaggtgaaag
    83101 aatcgttgag atattaaaac aaggtgagca tcaaccaata aaagttgaaa atcaagttat
    83161 gataatatat gctgttataa ataatcattt agaggatatt ccgatagata atatagctag
    83221 atttgaatca gaattgtatg catttgtaga taataattat ccggaaatat caagaaaaat
    83281 attaggtgga gaagacttca ctcatgattt aacagatgca ataaatgaat ttaaagaaaa
    83341 gtttgttgtt gaagtataat cctttttaaa aaaggaggta aattaattgg caggagctgg
    83401 aatcaaagca atcaaaacac gtatgggaac tgttgaaagt acaaagcaaa taactaaagc
    83461 aatggaatta gttgcatctt ctaagcttag aaaagcaaaa gaaaaggctg aagattcaag
    83521 agaatatttt aatactttat acaatacagt aaaagatatt gcccaaacaa cacgtggaat
    83581 tagaaatgaa tttttaaaag aaagagaagt aaaaaataaa tgttatgtag tagtagctgg
    83641 agacagagga cttgcaggtg gatacaattc caatgtatta aaggaatctg tctctcatat
    83701 gaaaggtaaa aaggaaacag tgattactgt tggaaagaaa gcacatgaat ttttttctaa
    83761 aagaaactac aatatagcaa aagatataaa tagtgttgaa gcttgtggat atgaagatgc
    83821 gacagaaatt tcacaaacta ttattgacct gtataaaaaa ggtgaagtag atgaagttta
    83881 tattgcttat acaaaattta aatctgcttt atcacaagaa gttgaaattt tgaaattgct
    83941 tccattagtc tttgaaaaag atacagacga acaaggaatg gcagtaaaag aagaaaaaca
    84001 aaatagatca aaagtacaat atataccatc agcagaagca gttttaagtt atattgtacc
    84061 tcagtttata tcaggtacag tatatggtgg aattttggaa tcttttgcat cagagcaagg
    84121 tgctagaaga actgcaatgg aatcagccac agacaatgcg aatgaaatgc tatctaattt
    84181 agagttaagt tataacagag caagacagtc agctgtaact caagaaataa cagagatagt
    84241 tggtggagtt gaagctctaa aatagggagg ttaggaaatg gcaaatgtag gtaaagtagt
    84301 acagattgta ggagctgtac tagatgttaa gtttgatagt gaacaaagcc tacctaactt
    84361 attaaatgcg ttagtaataa agttaggaga taaagaaata gttgcagaag ttgctcaaca
    84421 tataggtgat gatactgtaa gatgtatagc tatgagtgca acggacggac ttgtaagagg
    84481 tatggaagtt gtagatacag gagggccaat aagtgtccct gttggagatg agactttagg
    84541 tagaatattc aatgttcttg gaaaaccagt tgatgggaaa ccagctccaa aatctgcacc
    84601 taaattacct atacatagac cagcaccggc gtatgatgaa ctagaaacta cagcagagat
    84661 actagaaact ggtattaagg tagttgactt actagctcca tatctaaaag gtggaaaaat
    84721 aggtctattt ggaggagcag gagtaggaaa gacagttcta atacaagagc ttataaacaa
    84781 tattgctaag caacatggtg gtatttctgt attttcagga gtaggagaaa gaacaagaga
    84841 aggtaacgac ctttatggcg aaatgagtga gtctggagtt ataaataaaa cagctctagt
    84901 atttggtcaa atgaatgaac cacctggagc gagaatgaga gttgctttaa ctggacttac
    84961 aatggcagaa cattttagag atgagcaagg acaagacgtt ttacttttcg ttgataatat
    85021 attccgtttc acacaagctg gttcagaagt ttcagcactt ctaggacgta tgccatcagc
    85081 tgttggttat cagccaacat tagctactga aatgggtgca cttcaagaga gaataacatc
    85141 aactaagaaa ggttcaataa catctgttca agcagtatat gtaccagcag atgacttaac
    85201 tgacccagca ccagctacaa cattctctca cttagatgca aaaacagttt tatcaagaca
    85261 aatatcatca ctgggtatat atccagcagt tgaccctctt gaatctacat caaggattct
    85321 tgaccctagt atagtaggaa aagaacatta tgaagtagct agaggtgtac aatcaatact
    85381 tcaaagatat aaagaacttc aagatataat tgctatactt ggtatggatg aattatctga
    85441 tgaagataag ttaatagttg cacgtgctag aaagatacaa agattcttat ctcaatcatt
    85501 cacagttgct gagcaattta caggaaatcc aggtcaatat gttcctgtta aagagactgt
    85561 aagaggattt aaagaaattc ttgaagggaa acatgatgac ttaccagagt cagcattctt
    85621 atttgtagga acaatagaag acgctgttag aaaagcgaag ggaagtatgt agtcatggcc
    85681 agtgaatttt cattgaaagt tgtaactcca agtaatactt tttatgatgg agatgttgaa
    85741 atgatagtag ttagaactac aaaaggagac aaaggggtgt tgaaaggtca tataccattt
    85801 gtttcaggta tacctgtagg aactttaaag ataaaaaaag agggaagttt taaagaggcg
    85861 aaaatagcag aaggattcat taatgtaact caagataaaa ctgtcatatt gacagaatct
    85921 gctgagtgga tttaataaat taatttagag tataaaaagt cattttgacc aaaaaaactt
    85981 ttgggtaaaa tggctttttt gtgttttggt gttaataaaa taacgataaa attgtaagtt
    86041 tttagtgata ataaattcac aaaatgttta aatttactgc caaattatgt tgtaattaat
    86101 aagataaaga agatttagat agcttttatg taagtgaaaa agtcttcata taagaaatac
    86161 atataaaatt tttataaaat ttaaaaaatt tgcttagttg tgtagtataa taaataatat
    86221 aatagatttg tatataatat tcattacaaa taattttaaa agttacagtt ttattataat
    86281 gattattaaa ttaagataag taacataaac taaataataa tgtgtattat cgatagtaaa
    86341 tttataaatt cattattaat tattgaaaga agtattataa attttctgga ggaacaatga
    86401 atatatttga tattggcgta gatataattg aaattgatag aataagaaag gctgtagaca
    86461 aaaataatag gtttttggag aagatattta cagatagaga aatagagtat tttaattcta
    86521 agaattttaa agcagaaagt atagcaggta atttcgcagc caaggaagct ataagtaaat
    86581 ctataggtac aggaattaga ttgtttaatt ttaaagatat agaagttctc agaaatgaaa
    86641 tgggaaaacc aatagtaaaa acttataata atctagcaaa aatgtgcata gattataatg
    86701 ttttagaaat aaaagtttca atatcacatt caaaagatta tgctatagca aatgcgataa
    86761 ccataattaa ggactaagga gtgattacat tatgatggat aaaacagcaa aagaaataat
    86821 gacaacagat gtaatagtag caaaacaaga tgatagtata gcagatgtag ctaatatgct
    86881 aattgcagaa aaaataggag gtctgccagt tgtagattca gagaatagag ttgttggaat
    86941 aatttctgaa acagatattc ttaaaaaaga aaaatatata gaagcacctc tctatataaa
    87001 tctattacaa ggattgattt tcttagatga cttaaaaaaa gttgaaaaag atataaaaca
    87061 agtagctgct tataaggttg gagaacttat gtctaaagat ataataaagg ttcatgaaga
    87121 tgataagttt gatgatgtag ctaatgtaat gattaaaaaa tcaataaaca gagttcctgt
    87181 agtagatgat gacaacaaac ttaaaggtat aatttgtaga tatgatataa taaaagcttt
    87241 atacaatgaa taaactgaag taggaggtgt gatgtgagaa aaaagtggac catagtatgt
    87301 attatgttct tggcactggt tataattgta ataggatgcc aaaaacgaca gtccacaaaa
    87361 gaagaagtat acaaagattt tcaaaagcag atttctgata tgaattatta cagttgtaaa
    87421 gctgaagtag aagtagttgg aaataaaagc ccacacaatt atgttttaat tcatacttat
    87481 aaaaaaaccg ataactataa attagaagta atttccccaa aacatttaaa agggaaaagt
    87541 atagaatatc aaggtgataa aatattagtt aaaaatccaa agattagtga tgtagtggag
    87601 ttacctaata caggaaaaaa taatcaatat ctttttgtag gagactttat aaaaaactat
    87661 cttcaaaatg aggagatgaa agtaaagtta tccaaaggac atttggtttt agagactttt
    87721 attcctgggg ataataaata ttttaacaag caagtattat atgtaaatgc tgacacaaaa
    87781 aatcctgaaa aaatggaagt gttagataaa gagggagtgc caagatttac agtaaaatac
    87841 aaagattttg aatacagaaa ctaaggggga ctaaagacat gcaaaaaata acagtgccta
    87901 catgggcaga gataaatcta gataacttaa gatttaactt aaataatatt aaaaatttat
    87961 tagaagaaga tattaagatt tgtggagtaa taaaagctga tgcatatgga catggtgcag
    88021 tagaagttgc aaaattgcta gaaaaagaaa aagtagatta cttagcagta gcaagaactg
    88081 ctgaaggaat tgaacttaga caaaatggca taacacttcc tattttgaac ttgggatata
    88141 ctccagacga agcttttgaa gattctataa aaaataaaat aactatgaca gtttattctt
    88201 tagaaacagc acaaaagata aatgaaattg caaaatcttt aggagaaaaa gcctgtgttc
    88261 atgttaaaat agactcaggg atgactagaa taggtttcca acctaatgag gagtcagtac
    88321 aggaaataat agaattaaat aaattagaat atatcgattt agaaggtatg tttactcatt
    88381 ttgctacagc tgatgaagta agtaaagagt acacttataa acaagctaat aattataaat
    88441 ttatgtctga taaattagat gaggctggtg taaaaatagc tataaaacat gtatcaaaca
    88501 gtgcagctat tatggattgc cctgatttaa gattaaatat ggtaagagca ggaataatat
    88561 tatatggtca ttatccatct gatgatgtat ttaaagatag attagaatta agaccagcca
    88621 tgaaattaaa atcaaaaatc ggacatataa aacaagttga accaggtgta ggaataagtt
    88681 atggactaaa atacacaact acaggtaaag aaacaatagc tacagttcca ataggatacg
    88741 cagatggatt tactagaatc caaaaaaatc caaaggttct tattaaggga gaagtgtttg
    88801 atgtagttgg tagaatatgt atggatcaaa taatggttag aattgacaaa gatatagaca
    88861 taaaagttgg agatgaggtt atactatttg gagaaggcga agttacagct gagcgtatag
    88921 ctaaagactt aggaactata aactatgaag tgttatgtat gatatcaaga agagttgacc
    88981 gtgtttatat ggaaaataat gagcttgtac aaataaacag ttatttgcta aaataaaacc
    89041 atgatattat aatctgggag gcgattttgt gtacgataag agtaaagaaa aaatagaagt
    89101 tactttacct gactctctga tttctgaagt tgatagtata gttcagatgg agaacagtaa
    89161 cagagaagat tttgcaaaag ctgcttttca gttctatata actcagaaaa agaaaattca
    89221 tattaaagaa tctatgaaaa cgggttatag agaaatgggt caaattaatc tgtcactagc
    89281 tgaattaggt atgacagttg aagaagtatc ttctgatgat aaaaatgaag ggaagattgc
    89341 ccaaggtgag tactaattgt gaaataaagc gtggtgattt atattacgct gatttaagtc
    89401 cagttgttgg ttcagagcag ggaggcgtta gacctgttct gataatacaa aatgatgtag
    89461 gtaataaata tagtcctaca gttatagtat cagctataac atctcagatt aataaagcca
    89521 aattacctac tcatgtagag ataagttcca atgaatatgg gcttaataaa gactcagtaa
    89581 ttcttttaga gcaaattaga actatcgata aaaagagact gagggaaaaa attggttgtc
    89641 tagatgaaaa tatgatgata aaagtagatg atggacttca aataagttta ggattgttta
    89701 ctttttaaag agggatttgt cttttattag acaatccctt ttttctttta aatatctctt
    89761 aaggtttaat tttaatgtac tatgttaata aaattaaaaa taacgcaaaa aaatagcata
    89821 aaagtgatat aataatatgg aattgatata aaattacgaa ctcaagggag gtaattgcat
    89881 gagagaccat aaaggattaa atgagattgc gcgtatcata agaagagata tagtttcaat
    89941 gatacataga gccaaatctg gacatccagg agggtcatta tcagtagttg aaatacttac
    90001 tgcgttatac tttgatgaaa tgaatgtgga ttcttcaaat cctaaaatgg aggatagaga
    90061 tagatttgta ttatcaaaag gacatgctgc accagcatta tatgctacat tggctgaaaa
    90121 aggctatttt gataaggaag aactgaatgg attaaggaag ataggaagga tgttacaagg
    90181 tcatccagac atgaaaggca cgccaggtgt ggaaatatct actggttctt taggtcaagg
    90241 tttttcagta gcatgtggta tggctatggc atcaaagttg gataatgctc cgtggaatgt
    90301 gtatactttg cttggtgatg gtgaggttca agaaggaatt gtttgggaag ctgctatgag
    90361 tgctgcacat tataaacttg ataatttaat tgctttttta gataataatg gacttcaaat
    90421 agatggagat atagaaagcg taatgagttt aggttcaata gttgataaat ttaaagcttt
    90481 tggttggaat gtaattgaga tagatggaca tgattttgac caaatatttg cggctcttga
    90541 tatagctaaa tcaacagtta aaaaacctac aatgatagtt gcaaaaacta taaaaggcaa
    90601 aggaatatct tttatggaaa atcaagcagg atggcatggg acagctccaa gtgatgagga
    90661 gctagaaaaa gccttgcttg aattaggagg tgctgacaat gagtaaaatg gcgacgagag
    90721 aagcatatgg taaagcatta gtaaaattag gtcaaataaa tgacaatgtt gtagtattag
    90781 atgcagattt atcaaagtct acaaaaacac atgattttta caagtcattt ccagatagat
    90841 tttttaatat gggtatagct gaacaaaatt taataggcgc tgcttgtgga ttatcaactg
    90901 ctggtaaaat accttttgca agtacttttg ctatgtttgc tacgggaagg gcttttgaga
    90961 ttataagaaa ttcagtttgt tatccaaagc taaatgtaaa aatatgtgct actcatgcag
    91021 gtcttactgt tggagaggat ggagcatctc atgaaagtgt ggaagatatt gctataatga
    91081 gagctatacc aaatatgaca gttcttgttc cagctgatgg agttgaaaca gaaaaaatta
    91141 tttttgaaat agctaagtat aatggacctg tttatgttag attagggaga agttcagtac
    91201 cagttttatt tgatgaagat tataaatttg aaataggaaa aggtacagtc ttaagagaag
    91261 gaaaggatgt atcaattata gcttgtggaa ttatggtaaa tgaagctcta ttagcacaag
    91321 aaaagttgca agaagaaggt ataaatgcta gagttataaa catgtcctcg ataaaaccta
    91381 tagacaaaga tttaatatta gaatcagcaa aagaaacgaa tgctattgta actgtagaag
    91441 aacatagcat aattggggga ttaggttctg ctgtaagtga ggtagtagga gagtcttgtc
    91501 caaccattgt aaagaaagtt ggaattaaag atacatttgg agaatctggt actccaaatg
    91561 aattgttaaa aaaatatgaa ttaacttgtg atgacataat aaagactgtt aaagaagcta
    91621 ttattgcaaa aagaatgtaa gagatataaa ttattcatgt ttgttaataa tataatatat
    91681 ttaaaagaga ggtataaagt gttatattaa tttatatctc tctttttttt atatgatatc
    91741 aatatatcaa aaatgtaata aaatattcaa atttttacaa aaatagagaa aatattcaaa
    91801 tatatattga ctattcagaa aaatatgact aatatataat ctatagagaa actaatttta
    91861 tatataatga ctacttagat aaaagaatct aaaaaaatta tgagaggagg agtagggtat
    91921 aaaatcagat tataagtttt aagttattat actaaaaagc tataaaacag ttatcatgta
    91981 ttttatgtta aaggaaattg taggtagttt atcgaattat ttaatcagaa gaaaaaattt
    92041 cacatgggga ggtaactgag atatgtcaga aaaagtaaat gttaaaacag tcatcagttt
    92101 cgcaggtgcg tatgtagcaa cagtaatcgg ttctggattt gcaacaggac aggaaataat
    92161 gcaattcttc tctttttatg gttttgcagg tattattggt gggataattt caatggtatt
    92221 attctcatgg atgggtgctt cagttatgtc aaaaggtaaa gagcttcaac ttaaagaacc
    92281 tatcaaaatt tatcgtgttt attgtggtaa gtatttaggg atattctttg aatggtttgg
    92341 acctttattc ttatttggtg ttttcgtagt aatgatttca ggagcaggag caactttaac
    92401 tgaatactat ggattaaatc catttgtagg tagagtaggt atggcagtag tagcattatt
    92461 aactgtttca ctaggtcttg ataaattatc aaagatacta ggtggaatag ggcctataat
    92521 aataatcttt acacttttag ttggtggtat aagtttagct aaaaacatag gtaatatagg
    92581 agaagctgca caagttctta gcactgtaaa gatagctaaa ccagttccaa atgcctatct
    92641 atctggtgta atatatacta cttataacac aattgttgtt atggcattct taactggatt
    92701 aggagcaagt gctgctaaca aaaaagaagc tatttgtggt ggtatattag gtggagtagc
    92761 acttatggct gcagctataa tgatgcactt agctattctt tctgatatag gaaacttata
    92821 ttcaaaagca ataccatcat tattcttagc agataagatt tcaccagtaa ttggagtttt
    92881 attctcaata attcttatat taggtatata tacaacagct gtacctttat tatggtcagt
    92941 aactaataga tttgtagatg ataaacatcc taagtttaaa ttaataactt taatagcagc
    93001 tatattaggt cttataggtg gcttcctacc atttgataag ctggttggta ttttatatcc
    93061 atatactgga tatatgggcg tcataatact ggtttgtgtt ctttatagac agattacaaa
    93121 aacatctgga tatcaagaag gaatcagtga caaataagta ttttttaata ttgtaaactc
    93181 aagagctata tgaaaacaat gaaaatgttt tcatatagct ctttttattt tttagctcgt
    93241 ttattatgaa ataggaaaaa catgtttttt agttacatta catatattta taattatgga
    93301 taaatatata ctaatcaata atttagagtc taagataagc ttataaatca tatatttaga
    93361 atatatggga ggagtgataa gattgaatgt aaaatttaat attaaaggta taatttatgg
    93421 agttatagct cttgtattcg ttataggggg atttatattc ataccaagta tgtttgtaga
    93481 gaaatctaaa cccatagatt atacagtatt acaaagaaat tcaataccag aaaagatatt
    93541 agatgtgatg gataagtata caaatgaaga aagagcatta gcggctaaaa tcgataacaa
    93601 aatatacatt atagtgacta gaggaaataa taaatatggt atagaaatgg acaaaataga
    93661 aagtgtggtt gaggaaggaa aggatgtttt gagagtcaag ataaagtata aagataagga
    93721 agattctttt ccatacatag ttgttgaaac aaatatgagt gaacttccag atagaataga
    93781 attaaatgcg acgaaataag aaggtatttt ataattaaat aactgcatat tgtacaataa
    93841 agtcatggtt tttattaatg taagtaaatt gtatatggtt cttattgttg taaaagccat
    93901 gacttaaaat gcttataaag taagatatta acagtagtat ttattactat attaaatggg
    93961 gtctggaaat acttaatgtt ctaattattt ggagtgataa ttctagtttc agtaactatg
    94021 tagtctaatt gtgcatcatg agtttctttt ggaatagaat cgaatatctg gaagtcaaat
    94081 gctatgccaa tagttatagc atcttttctt aagttttcta aaaatctatc ataaaatcct
    94141 ccaccatatc ctaatctata gcaatgtata tcatatgcta cagctggtac aattacaaca
    94201 tctaaggcct taatatcaat tgtaggtgat ttttcgttag gttctcttat tccatatgct
    94261 ccaattctca atccctcttt taaatctgtt atttgagtag ggattaatgt atgattttct
    94321 tttattgtga tgggacttga tactattttc tttagagaca aaagtttttt gattaaatta
    94381 tctgtttgaa cttcattgtt aaaatttaga taaagcatta tattagttgc atttcttata
    94441 tcttccattt gaagtaattt ttcagttatt aaatttgaat tactaagtac aaagtttgca
    94501 ctcttatttt ttcttgattc aataactttt tttctaaatt cttttttcat aaaataattc
    94561 ctcccttaat atgttataat gtgtacagca attgtattta atataaattt tagtataatt
    94621 tattgtaata aagcaatata tttgggtaaa tgtaataaaa ataacagcta gtcatataat
    94681 aataatagtt tatgtaaaag gagatgtatc ttgtgatttc aaagaagaaa gctatttttt
    94741 taggggtgat tttagtaatt ataacagcta tggttacatc agcatttcag ttgacattgg
    94801 ggaataaagt tgtaatatcg aaagaactct atgaagacta taaaaaatat gataagttgt
    94861 taggactaga atctattata aaacaagatt tttataaaaa agtatcagat actgatttgg
    94921 taaatggagc agcaaaaggt ctatttctag gaacaaatga taagtattct ggttattata
    94981 ctaaagatga aatggagaat ctaattaatg atagtgaagg ttcttatgtt ggtgtaggta
    95041 tgtatatagg ggcatcaaaa gatggtggct tagtagtagt tcccatgaaa gattctccag
    95101 cagagaaagc aggtgtaaaa tcaggggata aattagtaaa agtcaatgga aaatctgtct
    95161 catataaaaa ttcagatgaa gctgttcgta tgatgaaggg taaaaaaggg aaaacagttg
    95221 aactgactat attaagagaa gataaacaat tgaactttaa agtaaaaaca gaccagataa
    95281 ttgagaagag tatagaaagt aaagtaattg ataatgattt aggatatata gaaattaccc
    95341 aatttatatc aagtacatat actgactttg ataaagcttt aaaagaatta aaagcaaaaa
    95401 atatcaaggg attagtaata gaccttagaa ataatcctgg tggaatgcta gatatatgta
    95461 aagaagttgc tgatgagtta attggtgaag gaactatagt atatacaaaa gataataagg
    95521 gaaatactga gtacttgaaa tctgataaag aaaaattggg acttccaata gtagtgttaa
    95581 ctaatggaga aagtgcttct gctgctgaaa tattaacagc tgcaatcgtt gataataaag
    95641 aaggtatttc tgtagggact acaacttttg gtaaagggtt ggtacaatca gttgtaagat
    95701 tgaaggatgg aacaggatac aagttaacta ctgctcaata ttttacacct aatggagatt
    95761 atatcaatga aaaaggcata aagcctacta ttgaagaaaa agatgaaaat aaacagttag
    95821 atgtagcgac taaatggctt agagaacaga ttgataaata aagtttaatt tttataaact
    95881 tatattgtaa aataaagaac cttaataaaa ggagtgtctc aaaattaact ttttagttta
    95941 tgaggcactc ttttgtgggt ctttaaaata aagtgtgtat tcttctaaaa agtagtgata
    96001 attaaaatat taaatatcac tatatataag gaaggtgcac actttttatg tttaacaaaa
    96061 acgaaatatt aaaaaaactt attgaagaat atgatgttaa aacaactaca gatattcaag
    96121 atatgctaaa agatttattt gcaagtacaa ttcatcaaat gttagaagcg gagcttgacg
    96181 accatttagg ttatgataga tatgacaata agaataaaaa tactaaaaac tctagaaata
    96241 gatatagaaa taaaaatgtt aaatcagatt ttggagaaat aactttaaat attccaagag
    96301 atcgaaatgg agattttgaa ccaaaggtaa ttagaaatta tgaaaatgat atatcaggaa
    96361 tagagaacca agtgattgga atgtactcaa aaggtatgtc tactcgagat atatcaagcc
    96421 accttaagag tatttacgga attgatatat ctcattctct aatagctaaa attactgata
    96481 gagttctacc tttagctcat gaatggcaaa gcagagctct tgatatggtt tatccaattg
    96541 tatttatggg tgctatacat tataaagtta gaagtgaagg tagaataata aacaaagctg
    96601 catatatagc cataggtgtt aacttagaag gtataa
//
LOCUS       DAMKPG010000003        89805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMKPG010000003 DAMKPG010000000
VERSION     DAMKPG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 89805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.003"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(93..1694)
                     /locus_tag="PC621_000233"
     CDS             complement(93..1694)
                     /locus_tag="PC621_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF4107424.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1805..2482)
                     /locus_tag="PC621_000234"
     CDS             complement(1805..2482)
                     /locus_tag="PC621_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107425.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2495..3190)
                     /locus_tag="PC621_000235"
     CDS             complement(2495..3190)
                     /locus_tag="PC621_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF4107426.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3242..4441)
                     /locus_tag="PC621_000236"
     CDS             complement(3242..4441)
                     /locus_tag="PC621_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF4107427.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4702..6747)
                     /locus_tag="PC621_000237"
     CDS             complement(4702..6747)
                     /locus_tag="PC621_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107428.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7158..7967)
                     /locus_tag="PC621_000238"
     CDS             complement(7158..7967)
                     /locus_tag="PC621_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4107429.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8051..8584)
                     /locus_tag="PC621_000239"
     CDS             complement(8051..8584)
                     /locus_tag="PC621_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF4107430.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8711..10786)
                     /locus_tag="PC621_000240"
     CDS             complement(8711..10786)
                     /locus_tag="PC621_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107431.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(10789..11535)
                     /locus_tag="PC621_000241"
     CDS             complement(10789..11535)
                     /locus_tag="PC621_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107432.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(11549..11944)
                     /locus_tag="PC621_000242"
     CDS             complement(11549..11944)
                     /locus_tag="PC621_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HDF4107433.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(12057..13079)
                     /locus_tag="PC621_000243"
     CDS             complement(12057..13079)
                     /locus_tag="PC621_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4107434.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(13076..13747)
                     /locus_tag="PC621_000244"
     CDS             complement(13076..13747)
                     /locus_tag="PC621_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4107435.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(13833..15182)
                     /locus_tag="PC621_000245"
     CDS             complement(13833..15182)
                     /locus_tag="PC621_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF4107436.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(15194..16576)
                     /locus_tag="PC621_000246"
     CDS             complement(15194..16576)
                     /locus_tag="PC621_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF4107437.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16820..18034
                     /locus_tag="PC621_000247"
     CDS             16820..18034
                     /locus_tag="PC621_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF4107438.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            18211..20124
                     /locus_tag="PC621_000248"
     CDS             18211..20124
                     /locus_tag="PC621_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107439.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            20395..21369
                     /locus_tag="PC621_000249"
     CDS             20395..21369
                     /locus_tag="PC621_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF4107440.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            21390..21665
                     /locus_tag="PC621_000250"
     CDS             21390..21665
                     /locus_tag="PC621_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF4107441.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            21678..22841
                     /locus_tag="PC621_000251"
     CDS             21678..22841
                     /locus_tag="PC621_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF4107442.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            23178..25457
                     /locus_tag="PC621_000252"
     CDS             23178..25457
                     /locus_tag="PC621_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4107443.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVNFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSVVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPMPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(25552..26892)
                     /locus_tag="PC621_000253"
     CDS             complement(25552..26892)
                     /locus_tag="PC621_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4107444.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(26904..27686)
                     /locus_tag="PC621_000254"
     CDS             complement(26904..27686)
                     /locus_tag="PC621_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107445.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(27701..28723)
                     /locus_tag="PC621_000255"
     CDS             complement(27701..28723)
                     /locus_tag="PC621_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF4107446.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(28729..29742)
                     /locus_tag="PC621_000256"
     CDS             complement(28729..29742)
                     /locus_tag="PC621_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF4107447.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(29870..30808)
                     /locus_tag="PC621_000257"
     CDS             complement(29870..30808)
                     /locus_tag="PC621_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4107448.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            31078..31542
                     /locus_tag="PC621_000258"
     CDS             31078..31542
                     /locus_tag="PC621_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107449.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(31654..31971)
                     /locus_tag="PC621_000259"
     CDS             complement(31654..31971)
                     /locus_tag="PC621_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4107450.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(31984..32715)
                     /gene="chbG"
                     /locus_tag="PC621_000260"
     CDS             complement(31984..32715)
                     /gene="chbG"
                     /locus_tag="PC621_000260"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF4107451.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(32741..34051)
                     /locus_tag="PC621_000261"
     CDS             complement(32741..34051)
                     /locus_tag="PC621_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF4107452.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(34128..35441)
                     /locus_tag="PC621_000262"
     CDS             complement(34128..35441)
                     /locus_tag="PC621_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4107453.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(35463..35774)
                     /locus_tag="PC621_000263"
     CDS             complement(35463..35774)
                     /locus_tag="PC621_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4107454.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(35974..36678)
                     /locus_tag="PC621_000264"
     CDS             complement(35974..36678)
                     /locus_tag="PC621_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4107455.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(36773..38134)
                     /locus_tag="PC621_000265"
     CDS             complement(36773..38134)
                     /locus_tag="PC621_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4107456.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            38633..39778
                     /locus_tag="PC621_000266"
     CDS             38633..39778
                     /locus_tag="PC621_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4107457.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            complement(39868..41586)
                     /locus_tag="PC621_000267"
     CDS             complement(39868..41586)
                     /locus_tag="PC621_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF4107458.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(41591..42580)
                     /locus_tag="PC621_000268"
     CDS             complement(41591..42580)
                     /locus_tag="PC621_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4107459.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(42676..43725)
                     /locus_tag="PC621_000269"
     CDS             complement(42676..43725)
                     /locus_tag="PC621_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4107460.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(44002..44670)
                     /locus_tag="PC621_000270"
     CDS             complement(44002..44670)
                     /locus_tag="PC621_000270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF4107461.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(44676..46049)
                     /locus_tag="PC621_000271"
     CDS             complement(44676..46049)
                     /locus_tag="PC621_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF4107462.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(46046..47824)
                     /locus_tag="PC621_000272"
     CDS             complement(46046..47824)
                     /locus_tag="PC621_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF4107463.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(47941..48264)
                     /locus_tag="PC621_000273"
     CDS             complement(47941..48264)
                     /locus_tag="PC621_000273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF4107464.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(48251..49228)
                     /locus_tag="PC621_000274"
     CDS             complement(48251..49228)
                     /locus_tag="PC621_000274"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF4107465.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(49243..49806)
                     /locus_tag="PC621_000275"
     CDS             complement(49243..49806)
                     /locus_tag="PC621_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF4107466.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(49858..50349)
                     /locus_tag="PC621_000276"
     CDS             complement(49858..50349)
                     /locus_tag="PC621_000276"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF4107467.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(50352..52277)
                     /locus_tag="PC621_000277"
     CDS             complement(50352..52277)
                     /locus_tag="PC621_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF4107468.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(52279..52590)
                     /locus_tag="PC621_000278"
     CDS             complement(52279..52590)
                     /locus_tag="PC621_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107469.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(53220..53495)
                     /locus_tag="PC621_000279"
     CDS             complement(53220..53495)
                     /locus_tag="PC621_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF4107470.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(53610..55019)
                     /locus_tag="PC621_000280"
     CDS             complement(53610..55019)
                     /locus_tag="PC621_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF4107471.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(55389..56051)
                     /locus_tag="PC621_000281"
     CDS             complement(55389..56051)
                     /locus_tag="PC621_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107472.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(56305..58524)
                     /locus_tag="PC621_000282"
     CDS             complement(56305..58524)
                     /locus_tag="PC621_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4107473.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(58800..61454)
                     /gene="adhE"
                     /locus_tag="PC621_000283"
                     /gene_synonym="adhC"
     CDS             complement(58800..61454)
                     /gene="adhE"
                     /locus_tag="PC621_000283"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF4107474.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            61958..62158
                     /locus_tag="PC621_000284"
     CDS             61958..62158
                     /locus_tag="PC621_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107475.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(62366..62956)
                     /locus_tag="PC621_000285"
     CDS             complement(62366..62956)
                     /locus_tag="PC621_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF4107476.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(62959..63834)
                     /gene="dpsA"
                     /locus_tag="PC621_000286"
     CDS             complement(62959..63834)
                     /gene="dpsA"
                     /locus_tag="PC621_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF4107477.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(63910..65301)
                     /locus_tag="PC621_000287"
     CDS             complement(63910..65301)
                     /locus_tag="PC621_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF4107478.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(65305..66441)
                     /locus_tag="PC621_000288"
     CDS             complement(65305..66441)
                     /locus_tag="PC621_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF4107479.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(66473..67018)
                     /locus_tag="PC621_000289"
     CDS             complement(66473..67018)
                     /locus_tag="PC621_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF4107480.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            67342..68121
                     /locus_tag="PC621_000290"
     CDS             67342..68121
                     /locus_tag="PC621_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107481.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            complement(68577..69005)
                     /locus_tag="PC621_000291"
     CDS             complement(68577..69005)
                     /locus_tag="PC621_000291"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF4107482.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     repeat_region   69195..70679
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=69261..69289
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(70863..71129)
                     /gene="cas2"
                     /locus_tag="PC621_000292"
     CDS             complement(70863..71129)
                     /gene="cas2"
                     /locus_tag="PC621_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF4107483.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     gene            complement(71132..72112)
                     /gene="cas1b"
                     /locus_tag="PC621_000293"
     CDS             complement(71132..72112)
                     /gene="cas1b"
                     /locus_tag="PC621_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF4107484.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            complement(72115..72666)
                     /gene="cas4"
                     /locus_tag="PC621_000294"
     CDS             complement(72115..72666)
                     /gene="cas4"
                     /locus_tag="PC621_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF4107485.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            complement(72681..75002)
                     /locus_tag="PC621_000295"
     CDS             complement(72681..75002)
                     /locus_tag="PC621_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF4107486.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            complement(75013..75714)
                     /gene="cas5b"
                     /locus_tag="PC621_000296"
     CDS             complement(75013..75714)
                     /gene="cas5b"
                     /locus_tag="PC621_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF4107487.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            complement(75716..76630)
                     /gene="cas7i"
                     /locus_tag="PC621_000297"
     CDS             complement(75716..76630)
                     /gene="cas7i"
                     /locus_tag="PC621_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF4107488.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            complement(76632..78509)
                     /gene="cas8a1"
                     /locus_tag="PC621_000298"
     CDS             complement(76632..78509)
                     /gene="cas8a1"
                     /locus_tag="PC621_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF4107489.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            complement(78515..79252)
                     /gene="cas6"
                     /locus_tag="PC621_000299"
     CDS             complement(78515..79252)
                     /gene="cas6"
                     /locus_tag="PC621_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF4107490.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            complement(79330..80247)
                     /locus_tag="PC621_000300"
     CDS             complement(79330..80247)
                     /locus_tag="PC621_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF4107491.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            complement(80526..82442)
                     /locus_tag="PC621_000301"
     CDS             complement(80526..82442)
                     /locus_tag="PC621_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107492.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            complement(82442..83188)
                     /locus_tag="PC621_000302"
     CDS             complement(82442..83188)
                     /locus_tag="PC621_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107493.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            complement(83292..84143)
                     /locus_tag="PC621_000303"
     CDS             complement(83292..84143)
                     /locus_tag="PC621_000303"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF4107494.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            complement(84391..85395)
                     /locus_tag="PC621_000304"
     CDS             complement(84391..85395)
                     /locus_tag="PC621_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4107495.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            complement(85405..86097)
                     /locus_tag="PC621_000305"
     CDS             complement(85405..86097)
                     /locus_tag="PC621_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4107496.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            complement(86411..87370)
                     /locus_tag="PC621_000306"
     CDS             complement(86411..87370)
                     /locus_tag="PC621_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4107497.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            complement(87403..88281)
                     /locus_tag="PC621_000307"
     CDS             complement(87403..88281)
                     /locus_tag="PC621_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107498.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            complement(88274..89056)
                     /locus_tag="PC621_000308"
     CDS             complement(88274..89056)
                     /locus_tag="PC621_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107499.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            complement(89057..89686)
                     /locus_tag="PC621_000309"
     CDS             complement(89057..89686)
                     /locus_tag="PC621_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107500.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
BASE COUNT        30063 a        15419 c        10112 g        34211 t
ORIGIN      
        1 cgaagcatga tttttattta aataaatccc aaacttcgtc tttacttact tagatattta
       61 tttactttct aaatcctatt atttttaaaa ccttatcttt ttaatacttc accacattta
      121 ttctcagtaa tctctccatt ttctaacgca atttttccat ttacaattac atattcaaca
      181 ccattggcta gtaattgtgg ctttgaaaaa tcaatatctt ctgacctaga ataactatag
      241 ttatccatgt caataactgc tatatcagct atcaaccctt ctttaataat acctctattt
      301 tttattttaa attgcctagc tggtaatgaa gtaactcttc ttacaatctc ttccaatcta
      361 acaccagact ttctgtacat attgaagaaa attggaaaag ctcctcttct ttgtagttca
      421 aaccatgaca tatccccacc cacactaaac aaacaatctg aaccaaccat aacaaatgga
      481 ttatcaatta ttttattatc atgtagttta tctgggaaat cagctctata cattcctcct
      541 aaacaaaaga taagttcttc gtctccatct aaaagtaaat cgagtaataa cttgtcctca
      601 tctattcctt tttgtgtagc tatttcacgt attgatttct tttccatatt aatatcttta
      661 gtgtttagaa gtattatact ccccctatct ccaagtataa atgcttcttt taatataagt
      721 gttcttccct cttcatttct aagatcatcc attaccccat ctatacctaa ttcaaataga
      781 ctagatgata ttgccataat aaattgaatt actctttttt tcttcataca atgcccagaa
      841 cttcttggta ctgtgtcaac cattatttca acaccattat acctagcctt taatatctca
      901 tcaaatgctt ctatttcaac tggacttaag tgagatactt gaaccctagc accacttttt
      961 tgcccaacct ctatggcttc ttttactgca ttatatcttc ctatgtaatc tctaatatga
     1021 gatgtatata taccatcatg ctctttaatc tgccttgcaa tatcaacaag ttcatcaata
     1081 tcagcatact tacttggtat atatgccaat ccagtagata ttccaaaagc accacttttc
     1141 attccatcat ttattatatc tgtaatttca tttttttcct tttctgtagg aggtctatcc
     1201 ttagaacctc ccataacact ccatcttata gtcccatgcc ccaacaagaa tcccatattg
     1261 acagaaatac cacttttaga tataaggtca caatattctg taaaattatt ccaacattta
     1321 tttttatcta taagatactt tttttcttct tcaagtaata gaccattttt atacatatat
     1381 tcatatacat tttcagaaga atatggtgtt atagaatgtc cacaattacc atttatagtt
     1441 gttgtaactc cttgttttag aaacttttca aatttaccat ctagtattgc tactatttct
     1501 tcatgaacat gagggtcaat aaatccaggt gttataactt ttccacaaca atcaatctct
     1561 ttatttgtat tgctatttat cttagaatct atttttgcta ttaagttgtc tttaattgct
     1621 atatctccat aaaaaccagg attacctgta ccatctatta taaatccatt ttttaagatt
     1681 aaatcatata tcaaaatatt ccctccttaa aaaccaattt ttaaataatc ttaatgtaat
     1741 ttttatattt gaaaatcaaa ttattatatt tcatatttga aaattcacta ttatttcatt
     1801 gtaattagtc actaagctta tctttataca tagctcttgc aattaatata gtaccaaata
     1861 cacatataag aactactaga gactgtggta ttataccttg ctttgataat ataatagtca
     1921 atacagatat tacaattgct actagaccta attttttatc ttgtaaaaat acttgtccaa
     1981 aaactgaccc aaatagagct ggtaaaatta agtttaattt ttctactact tcagctggaa
     2041 ttttagataa tacgcttcct cctaaaatta cccctatagt tagtatactt atattcacta
     2101 aaatagatac agctattcct attgtagata ttatcgagcc ttcttctgta cctgattcaa
     2161 cttctgctgc cttttgagct gctattgaac atggtagcct catattagat atattaccag
     2221 aaagaaaact catataagtt cctgggatac ctaagattgg tgaataagaa actggttcta
     2281 ttatgtaatt tggagcactc atactgacaa ttgccattgt acttgctaat aatgctgata
     2341 ttggaggcat aataccaaat ccaaagctca aaattattcc aggaactaat gctgctataa
     2401 tacctaaaga tagtgttagt cttccatacc ttatcataaa aggtaaatat tcttcattgt
     2461 atatagattt tactttactc ataaaactcc ccccttatat tactgaagct attatcattc
     2521 cgctaaacat tgatattgta agcccccact ctcttaacca ttttatattt ttcttctttt
     2581 caagcataca caatattacc attatagcca ttccacttat acatgcaatt gttccattat
     2641 caaaacgcaa caccctatca gcatttagat atgcaaatga acccaacatt gctcctagag
     2701 atattattgg tataaatgat ttcttgccac tagatataac attggttatc ttatctagtt
     2761 tatgtccaaa tagtaatgtg aatattatcc atcctaaaga acctaatatc atagtccaaa
     2821 ctgcatttgt aaatgcaagt tttgtcattt caggagaacc taaagttata cctaatgcat
     2881 ctgttccaaa tccagctgac attgattcaa aaattacaga acctataaat gataatctca
     2941 tccatgctat aggcccaccc atagttacta taagcgatac cattccagca aatatagtca
     3001 ttgaaggccc aatagctgaa atagcactac tcttaacagc tgactttatt tgtttatctg
     3061 ttattcccat tgttttacca actttgcaag attttatagc aaatataagg gattgtacca
     3121 ctactattga tactgctact gtactcgcta tccacaaaag tggatgatta gctaatttta
     3181 aataatccat ttataaaacc tccctataaa tcctcaaatt ttattaatat ttctaatata
     3241 tctatttcca aacctgtttt agtactctaa gtatatttcc acctagtacc ttctctattt
     3301 cctcatcact atatccatgc tttactaaat atctgacaat gttttttgaa gtttctgttg
     3361 ggttctcaag accttttaca tattctactt tgggatgttc ttttgtacct gttatttttt
     3421 ttatatcaaa tttagcagct agtactgagt gtaattttac atggtcgcca tatactgtat
     3481 ctggtccaaa agctacatgg tctattccta ctaaattttt aatatactca aaatgttcca
     3541 taaatgactc tattgaatgt tctttattct tctctgttat tgtagtatga ggtgctgctt
     3601 ctacacctat aacccctcct tttttagcac atgctattaa tacttcatct ggagttagtc
     3661 tctttgtgtt ccataaattt cttgccccaa catggcttaa tattattggg tccttgctat
     3721 attctattac atcaagtgta gtttgaactc caacgtgaga gcaatctatt gccattccaa
     3781 ttttattcat tctttcaaca gctttttttc cgaaatttgt aagacctcca tctttctctt
     3841 cttttagtcc tgaacctaat gcattggatt cgctgtatgt tatacccatt aaccttatac
     3901 caaatccata cagtatatct attctatcaa gttcattttc tattggtgct gcaccttcta
     3961 atgttggtat caatgctact ttaccttcac ttttagctct gcatatatct tctacttttt
     4021 cacactttat gacaaaatct tgatgagcta aatcacatag tctcatgcct aaatctatta
     4081 atatatcatc ccatttccac cctgaatttg atgttatcgt acatacacca tccattaaat
     4141 tatcaaatat agcatcataa tatcctcttg ataatgcttc ataagcgcat ctttgtttac
     4201 cttccctatt atattcaaat atatctcttt ctaggtgttc tggaaataat actggatgtt
     4261 catgtaatga tataaatatt ttttcatcca ctatactttt aaccttagct tcttgaatat
     4321 catctaattc aataaggtac tctttaactc ttttatctcc tttacacatc tcaatagatg
     4381 aaatatcttt tcctttttct aaataactat aggcttcata tcctttgtaa gctgacttca
     4441 taacaacatc ctccaagttc tacaattttt tatatttttc ctatgtagca attgatatgc
     4501 caaaatataa attttaattg tataataaat cttgcaaaaa ataaaaacag tagtaaggtt
     4561 gatatttact actgttttat taaaaataat cttcttagat tatttattca atatttttga
     4621 attatcttaa tcttaaagat aattttattt atttctatcc attataggga tttttttagg
     4681 gtgttttgcc tttttagtcc cctatatcac tcccttaatt tttacatatg atataaagtt
     4741 ctttccttca tcagttataa aactaccttg tttttttact ccagataaaa gtaaaccatg
     4801 gctctttaac ttatctattc tttttctaat ttggtctaca cttaaaacta tatctttttc
     4861 ttttaaaatc tcttgcagtt tatttcttcc taataatata tttttattat tccaagtttc
     4921 tatactgttc aatatacata tactttcctc aaagaaatta gattgtttga aatctaagat
     4981 aattgaatcg aaattttcat taactaaagt attgtttttt tcaaatctaa attgttctgg
     5041 caaatgctcg taatctactc tatcttcctc aactatagta tcaatataat aaattaaatt
     5101 tttaagctct cttacatttc ctggccattc atacaatgtt aaagcttgca ttgattcctc
     5161 tgtaaaacat ttgttgctat ttatttcatc taaaaaatac ttacttataa gaggtatatc
     5221 ttccttacgt tctctaagtc taggtatctc tatatgtaat acattaatcc tataatacaa
     5281 atcttctcta aaagaacctt ctttaatttt tttctttaaa tctttatttg ttgcagctat
     5341 aattctaaca tctattggta taattttact tccgcccatt ctcataactt ctttttcctg
     5401 taatactctt agcaatctct gctgtacatc taaagatata tcacctatct catctaaaaa
     5461 aattgttcct gtatgcgctc tttcaaatat acccattttt ccacctttta ttgctcctgt
     5521 aaaacttcct tcatcatagc caaaaagttc actttcaata agtgtatctg acaaagaaga
     5581 tagattaact gcaacaaatg gcttgttttt tcttagagat tcattatgta ttgcctgggc
     5641 aaatatttct tttcctgttc cattttctcc aagtatcaac acagaaaagt ctgtagttgc
     5701 tatttttctt gcaatattaa ttttttcttt tattatttta ctctcaccta caacactctc
     5761 aaatgtatac tttgatacaa aaccttttga ctgaagttta ttttgaatct tgtcctcaag
     5821 cacttgaatt gcacttatat ttttaatgct tataatactc tttattcgtt cattattttc
     5881 ataaacattt actttattaa ttatcagttt tttgttattt aaatttacta cttcatcatt
     5941 aacttcatct tcttgaaaaa atattttttt gacaacctta tcactaaata agtccataaa
     6001 gttattagat ataatttcat tttggtctat tccaactaga ttagaaaata ctttgttgca
     6061 atatataaat tttcctaatt tatcaacaga ggcaatacct tcatctataa tctccaaaaa
     6121 actcttcatg gttttattca tagttgtata atacctatat ccacttactg tatcttcatt
     6181 gtatttttcc ttaataatat gtaatttatc tatagatata tttagttttg taaagatttc
     6241 tataactgta tttatatcaa ttaccttatc acctatgtca attatttgct ttatgttttg
     6301 tggtatgctg tttctgcttc ccgtaataat tccaatctca catttagact tatcacaacc
     6361 tggataatat ggtattagat taatatgaat taatcctagt tttctaatta tcttggcagt
     6421 ttcatctgct gactctttat aagcatctat aaccataaca tcagaatcat tttctatact
     6481 tattatctga tttatatttt caatatttat agtacgatgt actattacaa taagtatatt
     6541 tttatcaata ttattatgta tatattcctt tatatcattg tctgaacata ctacaagctc
     6601 atagcttgac agttcaatat ttatcctatt tacatatacc aagttatcta tattacaaaa
     6661 ttctccaaat atattttcca attgttttga tatatctata tttattttgt catcagttga
     6721 tacaaataaa atattatgtt ttttcatagt cccacctcct gtttaacgag tatagaaagt
     6781 aataatttgg tctattgtag cactttttaa aaaagtttta aatcttaaaa atcaaatttt
     6841 gtaccattta ctattatttt ccataataaa ataatgtaag ctatcttttt tcacatataa
     6901 tttaatttct aaaacggtta gtttgacatt attggaatat aaacaaaaat ttaatataaa
     6961 ttcgttaata tattaataca tttttaagaa tgacaaggaa taaattataa caaattttag
     7021 gaatatataa taatagggtc tcagaactta gttacaagtt ctaaaaccct atttgtttag
     7081 tgtaaaaaac atcataaaat ttagcttaaa tcttttcaca taatattact ctttttctaa
     7141 gtgataataa aatatatcta ttcctctaaa aactgtaaaa attcctgatt aaatagttta
     7201 agctcatcat agaatatagc atgaccacta tactcaaaag tatataaaat tgaatcttct
     7261 attttttcat tcataaaaac tgcaaattca tacggacata tttcatctaa tttcccatga
     7321 aatataccag taggcactct tacacatttt aaatcattaa ataattcttc atctcttaaa
     7381 gatacagcag ttccaatagt gccaatacct gatgcactaa aaccaatatc attaaaccat
     7441 cttctaaagc tttctggtgg atttgatgca aatacttttt ctccaaaatc ggttaccatt
     7501 tctggtctat cagtacatgc ttgtgctatc aatttattta catcttcacg tgtcatacca
     7561 tatggaaatt ctggaggtct ttgtacaaat gatggtgcag cagctgcagc taataccaat
     7621 tttgacactc catatccatt aaataaactc atatacctaa gtacgattgc tcctcccatt
     7681 gaaaaaccaa caaaggtaaa gtcttttaat ccaatagcat gtaccacctt ataaatatcg
     7741 tctgctaatt ggctataagt atatcctcct gatgttgcat cagatttacc aaaacctctt
     7801 aaatcaattg aaactgttct atatccgagt tttggcaata tatttgtttg atattcaaat
     7861 atcttatgtc ctaagggcca accatgtata aaaagtacag tttttttagc actcggattc
     7921 aaatcatata ctgctatgtt tatatcattc acatatacat aatacatatc aacagcaact
     7981 cccttcaaga aaatgtatga tggttatttt atatatatga ataaaatgat gaataactaa
     8041 atttttaaat ttatttacaa tacttctcta ttgccttttc aaataaactt acatgaagtt
     8101 tttcatctag gattattcta tttaatatag cttttatata agggtcttca ataagactta
     8161 tatctttata ataattatta atagccacat gctccaaatg caaatctagt tttaatctgt
     8221 tacaaataga atttccataa catacaaaac caccattcca atatgcacca caagaacttg
     8281 tagaacctct atatacagga tttccaccca ataatcttat agtctttgct aatatctcca
     8341 tatgtaacat ttctgttata gatatattta tccacatttc acttaactct ctatgcgttt
     8401 catctaaatc aaaattatgg tatagatatt gatttacact cgtaaattca cttgatactc
     8461 cggcatagtc atccatcaat aattctgcat aatacttatt tagacctaaa acttttatct
     8521 ctggatatgg ctcatcactt gaatatccat ttcgcttgtg catgtcgtaa tgtgcatcac
     8581 tcataatatc tcctccttta aatttaatac ttgatattaa ttgtatgcat cacgactttt
     8641 tcatataact tagataataa aatctattat attattttct attataattt atgtgtcaat
     8701 tcacccaaat ttatttgata tgcaatattt ctttccttag tgctacatat gtaataaaaa
     8761 catatataca atatattact acaaaattaa taagcgtaat ggctacagca tgtaagatag
     8821 gaatctgaat attaaaaaat actggagcat caaaagaaat attaaacatg ttgtatgaaa
     8881 aaactgatac caatattcca ccaaatatta ctgcaggaat taatggaatc gaaaaatata
     8941 aacaaacatg tttctttaag atttttatta cttcactatc aatcatcccc aaagttcgaa
     9001 gcattccata gtgttttatg ataatgttat tttcgctcaa taattgaata gagagaatga
     9061 ctgtaccaat aatgcatata atgagtgcca aataaaaaag tggtaatatc aagataatgg
     9121 cttgaccagt agattgcata gcatactttc cacttatata atccttatta tcatatcgta
     9181 atttagaaac aaagccttga ttacctgcta ctactactcc agaattaaga ttttctaaag
     9241 tatcaaactt tttctgaaac tcagtaaaat aagcactatc caaaggctca cttgtaatag
     9301 caacataaag actatacaga ggtttcatat tatcaattaa ataatctgga atgacaataa
     9361 tatattcttg tccatttcca tatcctccat actgtgaaaa gttctctata taaatacctg
     9421 caatacttaa gtccttatct gatattgtca gtttatattt ttctccaata taattcttat
     9481 atgcatcagc aagataaggc atacaatgga tgataaattg atttttgtcc atctttattg
     9541 ggtttaaacc caacatattt cttagtatat tgtaatcact atatttcata aatgtgtcaa
     9601 actggttttc tgcaaaagta taatccttat attgtaaatc tcgccctata tctttcaagt
     9661 atttagaaag gatattatta cgaaccttca taaaggatgt tttttcttct gtgtaaagag
     9721 tataagaata atcctttttc acacctttcg tttctgataa atatccttca tatgaagaaa
     9781 aatcatatga ttcattcggg tgaaaaatac tcacatcaaa ttgttgtaaa tccactgatt
     9841 tattcatcac agaataaaat gagttagcta ttccaataca agcaattgat actgtaaaaa
     9901 gagttgcgat aatacccatt ataatgctta ttccatttat ctttgaagcc agtagacgat
     9961 acaaaaataa gtgagatttc ttatatttcc aattcatccc tttttcaaac atattctgtg
    10021 caatagatcc aaatcccata aataatacga ttagacaaaa tacaatcaac aataaaccaa
    10081 gaaatacatc tgttaatcta ccactaggta ttgaaaagaa aaaaataccc atacctccac
    10141 acaaaacaga cacacaaaac ataatagact tcgtttttgt ttggtgaatt aatacatttt
    10201 cattatatcg atcataatta agcaaattaa ttaatttcat tttaacaagt tttttattac
    10261 tttttaattg agcaattaaa tatattatgc agaagtataa aaaagtcatc ccagcagctt
    10321 ttaatgaaaa atccggagat aaagaatact ttccatgaaa catattaata acaatagatt
    10381 ccaaaacctt agaaacaaat atccctagaa tccatccaac aataagagca gaagcaccta
    10441 ataaaagatt ctctataaaa agcattttaa caatttgttt tttatctact cctaatatca
    10501 tataagtact tatttctctg cttctttttt tcaatataaa attcatcata taactaacaa
    10561 accagcctaa aataaaagta ataatgaacg aaaatatgat aatcatataa gaaaactcat
    10621 cgcttcctcc attattaagt atactagaaa gttcttgcat tacgtcggaa aatatcaaag
    10681 cgttaaaagc ataaatcaaa gctacagagc ctattaagga acaccaataa ataaggtact
    10741 ttcctgcttg tcgtcttgca ttacgaaaag ataatccttt caacataatc aatctcctcc
    10801 catcaaagaa agtgcatcca aaatttcgtg atagaaagta agtctggatt tttctccttt
    10861 tctaagttcg ttaaaaattt tcccatccct tagaaataaa attctctcag tataactagc
    10921 tgaaatagcg tcgtgtgtga caagaagaat cgtagcctct aatatggtgt ttatagtttc
    10981 caaaagttcc attaaagttt tagatgaatt agaatccaat gcaccagttg gttcatctgc
    11041 caaaataagt ttgggctctg ttacaactgc acgagctact gcacaacgtt gcctctgacc
    11101 accagatact tcataaggaa acttcgaaag tatctcttca attttcaatt gtgtggcaat
    11161 attatacaca ctattagtaa tttcaacagg agaaactttt tttattgtaa gtggaagtgc
    11221 tatattttct tctaatgtca gagtatctaa taaattagaa tcctgaaaaa caaaacctaa
    11281 attatcacga cgaaaaacag ttaagttttc ttcggatagt tcagtaatat cttgattttc
    11341 aagataaatt tttccagcac ttactgtatc aattgtagca atacaattta acaaagtagt
    11401 tttaccagag ccagaagttc ccataatact gacaaactct cctgtattaa tttgaaatga
    11461 cacatcattc aaggcctttg taagtatttt ctgattgcca taatatttct caatgttctc
    11521 tactcgtaat agcatgttaa agtcctcctt agtcaacaat ttttccattt ttaattatta
    11581 ttgtcttatc tgaaaaattc aagatatata catatatgct ttctttagag tcttgaattt
    11641 gaatagtagg ttcattagat gaaattagag atttttttac ttcaatatcg tattttattg
    11701 ttaattcatt atcatctcca tttttattag tatcttctac atttttattt aaatcaatgt
    11761 tattatgatt aaagggacca gtaaacttta cttctgtaat agttccattt tttctaataa
    11821 aagaagcata ttcagacctt agtggtatga tacttcgtga gaaaaaaccc atcgaatcca
    11881 atcctgtaat tgaaataaac aaaacgagta caacacataa tatagttaat atcctttttt
    11941 tcatatgttt ctccctcctt tctattgata ttgtagcgta aatagtaatc tgaatatata
    12001 gaagttactt aactttatct tacagttttg aaagataaag aaatagcagc gcatttttag
    12061 ttgcactgct tctgcatatt gatagctgta tttattggga aattcaatgt tattgctgtg
    12121 tattcaccaa ggctggagga cacatctata tcaattccca ttttcttaga cagttcataa
    12181 caaaggtaaa gaccaatccc tgtagaattg tttgtagtcc ttccattact gccagtaaaa
    12241 cctaattcaa aaattcgata aatttcactt tctttgatgc caataccatt atcttttata
    12301 gtaaacttaa cactcttgtc ttcttttatt gcaaaaaatg aaatttcagc tgaatcactt
    12361 ttgtactgaa cactattgat aataatttga tttaaaataa aaactatcca ctttttatca
    12421 gtatatacac tatactccaa atcctgtata ttaatatgaa tattattttg tataagaagc
    12481 aatttattct ttgctaatac ttcttctaca attgacttta atgttatttc agaaattaaa
    12541 taatcttttt caacatggcc aaggcgagca tagtacaatg cacgctcaat atgacgttct
    12601 atttcttcca tttgaaaacg aatttttcgt gtatgttcat ttttataatt ttcacatata
    12661 agttctgatg ttgtaacagg atgttttatc tcatgtaccc attgttcaat gaattgtcta
    12721 tattcgtaca tttcacgatg tatttgagaa acatgatctg tcatagattt caatgataat
    12781 ctaataatat tgtagtattc acgttctatg ttagaagtag gtttttcaat catttcaata
    12841 aacagatatt ttttatccaa ttcgtctaca atgcgacgta acttaataat ttcttttttc
    12901 tgtttttgat aacaaaaaca tatccaacaa aataaaattg cgacccaagc ggctataaga
    12961 agagtcaact ctccttgacc aatccctata agatgaagat atagcactaa tgaaattgca
    13021 caacaaatat ttaagagtat aactaatact ttatcttgaa gaaaattaca aaacctcata
    13081 tcctatatcc catcccccgt tttgtaatga ttaagtcagt aatacctata ccccttaact
    13141 tttctctaag ccttgtaatg tttacactta aagcattatc atcaatatga atatgattgt
    13201 cccataaata ttctaccaaa tctatacgag gcacaatact ccctacattc tgaaacaaat
    13261 aatacatgat ttttagttca gttcttgaaa gttctataac agcattttgg tattctactg
    13321 tagctgaaat aaggttgagt ataatctctc tatatttaat tttctgttta tctgatgaat
    13381 ctactctttt aagtaggttc tttacccttg ctaaaagcaa aggtaagtta tatggttttg
    13441 taatatagtc atctcccccc atagtcaaac catttagttc atcaattgca ctatctcttg
    13501 ctgtaataaa aataataggt atagtagaac aattcctaat ctgtctacat aaagaaaaac
    13561 catctatatt aggcaaattg atatccaaga gaattaagtc aggtgttgat tcttggatta
    13621 cttgaagtac atcacaatta tttgtatcca atgtttgtgt actatagcca ttattttcaa
    13681 agaattgcat taattcctta cagatgaaca catcatcttc aattatcaaa atattggtta
    13741 aaataataaa tacctcattt ctttagttta tattgcttta attttgtaag taatatattt
    13801 ttccatatat tacttacaaa attacttttt aattattcat cttttccagc tactgatagc
    13861 tctttaataa caactgaagg tgaacctata ctactcattg gaaattctaa atcttcccct
    13921 ataacttcaa tatctttcaa caggtcaaaa tagtttcctg ctactgtaat ttgctccact
    13981 ggaaatactt tttttccttc tttgatataa aagcctttgg cagctaaaga aaagtctcct
    14041 gttactgaat ttgcacctga atgaagccct gcaaagctag taaccatcaa tccttcatct
    14101 atatccttta taatttcatc taaagattta tctcctttat ctatataaaa attagttgga
    14161 tatatgctta taggtgatga atatgaagat tttattccat ttcctgtagt ctttacacct
    14221 gccttattag ccgtttttaa gttatgaagt aaagttataa gctttccatt taaaacaact
    14281 tcttttttaa atgtagctac tccttcatca tcaaaaggag tagatgccat accattttct
    14341 aaaagtgggt catcaactat tgtaactata ggagatgcta tcatgtctcc ttctctattt
    14401 ttcaataaag ataatccttt ttgagctgaa tctgcattaa atatcccaga aaatgtgcta
    14461 agcaaagata ccatagcttc attaagcaat atagttttat atttacctga tggaatactc
    14521 ttaccaccta ctttagataa agcattatca acaccaagtt ttgatatctc acaaggtttt
    14581 atttcttcaa tagaatttgc tctattatat cctattccat catatttttg tccattgtct
    14641 tctatgatag gaactacaaa ccctataagc atattagttt tattgcttaa attaagacct
    14701 ttagtattag atataccatt acttgataca gaataagata tagtacattt agataaattt
    14761 accaccttat ctgaataagc tttagtctct ctttccattt ctaaagctaa atcaatcaac
    14821 ttatctgcct ctaaattttc tagctcttta gaataagtct ttacatcatt atagtgctta
    14881 tctccttcat atataaattg tacatcttca ttttcaatag tgtttactcc atcttttaca
    14941 ttttttatca acatatcaac agctttatca tctaaaattt ctgtataaga ataacctatc
    15001 tttccattta ccttacctct aaaagataaa ccaaaagatt tatcaagatt gtatttctca
    15061 acttctcctt catatatatt tatacttaaa ttttctcctg tataataata tatttcacac
    15121 tcttcaaaac cttcacttag agccttttta aataatatat ctttaaaatt tttaagttcc
    15181 acaattatcc ctcctatctt ccacctacag taatttcttt tacccttatc ataggttggc
    15241 ctacattagt tggtatactt cctgatgatg agccacacat cccctgagct tgttttacgt
    15301 tattcccaac catatctata ttcataagta tatcgctacc ttttccaatt aaactagcac
    15361 ctctcactgg ctcttgtatt tctccatttt ttattaagta accttctgaa actgcaaaat
    15421 taaactctcc tgttacagga tttacagaac caccacccat ttttttagca taaagtccat
    15481 ctggtattga ctttataatg tcttctggtc tatcttctcc agcagcaata taagtgttag
    15541 tcatccttga agttggtgca aatttgtagt tctgtctacg agaacttcct gtaggattca
    15601 ttcccattcg tctaccatta agtttatcta tcatataaga cttcaaaatt ccattttcaa
    15661 tcagtatatt cttttgagtt ggatttcctt catcatctat attcatagaa ccccagtagt
    15721 ttggtattgt accatcatct atagctgtaa tctttgtgga agcaatctgt tgacctaatt
    15781 tatctgcaaa tacagagttc ccctttgcaa ctgatgtagc ttccaaagaa tgtccacaag
    15841 cttcatgaaa tattactcca ccaaatccat tatctattgc taccatcata ttacctgcag
    15901 gacaattttt agcatgcaac atagtatgcg ctactctagc tgattctttt gcataatatt
    15961 ctgggtctat ttcttcaaat atttcaaacc ctttacaacc tccaggacct tcaaatccag
    16021 tttgattttc attaccctta gatgcaattg aacttatccc tagtctggtt cttattcttc
    16081 tatcctcaac atatatacct tcagtgtttg caattaatat tttttggtct ttatccaaat
    16141 agctaacact tacttgagat atatcattac taaattcttt tgcactctta tatgcaacct
    16201 tcataacact tactttatct ctatttccta ttgaatttgg ataaattttt atattatgta
    16261 tgttatttat tttaatactg tcatttaata ttacactttt tccatcttcc aatttaccta
    16321 aagcaacagc agctttgtat gctacatcca acagactatt taatgaattg tcacttgtat
    16381 aagcatatat actcttcaat cctttaaata ttcttattcc tataccataa tttcttccac
    16441 ctattgcatt ttctacttta ttatcaatta aactaataga attatttata gtgtcttctt
    16501 caaaaatctc agcaaaatct cctccagtaa ttagacactt ttctaatacc ttagaagcta
    16561 tattttttga aagcataaat ttacctccta ctttaaattt ttaattaact tttattcaat
    16621 ctttttactt gttaacaaac attatatact atattactta aaataaggta attatgaata
    16681 tattatacat atgttttata ctaaaatgtt atcacatttt atctatacat acccatataa
    16741 ctagataatt aaaatgtata atattatttg ctatagaaaa taatacactc atacataata
    16801 aattttagga gtgaagtata tgaatcaaga atatgtaaaa ggaaatccta tttttactaa
    16861 tattaataaa gttccaagac aatttccata ccttactgat gatattgata ctgatgttat
    16921 aattgttggt ggtggcgtaa ctggatgtat ttgtgcttac tatctagcta aaaacaatat
    16981 aaagtctgtc attcttgaaa aaggaaggat tgcacatgga agcactagcg tgacaacttc
    17041 tctattacag tatgaattag atgacaattt gatagattta acagaggtta tgactctaaa
    17101 agatgctcta aaagcatata acctttgtac ttcagcactt gaagaattag atacatttat
    17161 aaagctatat ggaaataagt gtgattatgc caaaagagat actctacttt atactgccaa
    17221 caaacttgaa attaaggcta tcaaagaaga atataactta agaaaagaaa atggatttga
    17281 tgttgaatat attgatgaat ccacaaaccc ttttagcttt gatttaaaat caggattgat
    17341 agcaaagaat ggaggacgag aattagaccc atataaatat agtcaccatt tgattgatgt
    17401 aagtttaaaa aatggtcttc aagtatatga aaatacagaa gtaaaaaaag tagatttatc
    17461 aaatgataag gttactgctg aagtatcgta tggatacaaa gttcatggta aaaaattaat
    17521 tgtagctaca gggtacaata caagtttatt tacaaaaaga aattttgcaa ctaagtcaaa
    17581 tacttttaat atagcaacta aaccacttaa aaacatagct tcttggaaaa acaacatttt
    17641 aattagagat aactgtgacc catataatta tctaagaact actaaagaca atcgattaat
    17701 tataggtggt gaagatgtaa gttttgatga tatagaaaat gaaactttag ccaatgaaaa
    17761 gtatgatatt ttagaacaaa gattgaaaag tatgtttaag gatataaaag acattgaagt
    17821 tgaatataaa tattgtggtt gttttgcttc aactttagat aatttaggtt ttattggtcc
    17881 agataataaa aataacaatc tatggtattg cttaggttat ggagcaaatg gtatactctt
    17941 tgcaatatta ggaggtataa tgttaagtga attatatctt ggcaaacaaa ataaaaatat
    18001 gaaattattt aaagtagata gatttgataa atagatattg tatattaaaa gactataaaa
    18061 agatgaaact ttataattct aaaagttaac tttgatacag tcttttataa tgttcctttt
    18121 aaaaatactt tatttataat taaatataaa gtaaaatgtt aaaatataac tgataaaatt
    18181 tagtcataag ataatattta agtttatttg atgaaatatg gaggaattat tatgaaagaa
    18241 atactcgtta ttgctcccac taaaggaacc tatgaaaaat caattcacat agttaaaaaa
    18301 aacaaatata caaatattga tgttgtattt ggcaatctaa aagaaggtat acctcttgct
    18361 gaaaaaagta ttaatcatgg tactagaatt atcataagca gaggtggaac ttacaatatg
    18421 ttaaaagcta cttacaatat acctatagtt gaaattaaag tagatgctta tgatattata
    18481 aaaagttaca aagaggtaaa aaattccaac gaaccatttg gaataattgg atttaataat
    18541 gtcatctacg gtttcgatat tatagaagaa atattgaata agaaaataac tatgattgaa
    18601 attgaaaaag aagaagaaat ttatgatgct atagaaaagt acagaaagaa aggtataaac
    18661 acatatatag gagataccac tgttgcacat attgttaaga gacttaattg taagggaata
    18721 ttaatcgaat ctagagaaga aaacatactt agagccattc aacaagcaga acaaatactc
    18781 gaagcaacca aggatgaaca aaaaagaaga ttacagatag aagctatgac agattttgta
    18841 catgatggaa taataacagt tgataaaaat tttaaaataa cattatttaa taagagtgct
    18901 gaaaaaatat ttggtataaa aaaagaaaat gcactttatc ataatgttgt agatgttatc
    18961 ccaaacacag ttcttccaca agtagttgag actatggaac ctcaaattgg tgaaatacaa
    19021 aatataggga gtacaaaaat tattacaaat agagttccaa taattgttga tggccagatt
    19081 caaggggctg tttcaacatt tcaagatagc aaggagataa gttcttttga acatacaata
    19141 agaaggagct tattaagcaa aggtctatct gctaaataca cttttgataa tataattcat
    19201 gctagtgaaa agacaaaaga ttgtatagaa cttgcaaaaa aatatgcttg ttatgatact
    19261 cctatgatta ttactggaga atctggagtt ggtaaagaat tattttgtca aagtatacat
    19321 aactatagta agcgtaaaaa tgcacctttt gttgctgtaa attgtgctgc catacctcca
    19381 tctctaatag agagtgagtt ttttggttat gaggaaggtt catttacagg tgctagaaaa
    19441 aaaggtaaag caggtgtatt cgaactagca catgaaggta caatcttcct agacgaaata
    19501 agtgaaatcc ctcttgaact tcaaggtcga ttattgaggg tattgcagga aaaacaagtt
    19561 atgagaattg gtggtgataa agttattcct atagatgtaa aaattatctg tgcttcaaat
    19621 aaagacttaa aaaatatgat gcgcaatggt ttatttagac aagaccttta ttttagaata
    19681 aatatactaa cattatatct tccttcatta aaagaaagaa aagaagatat tataaaatta
    19741 tcagaatttt ttatacataa atattcaagc aaatacaata aaacgccttt aattatcact
    19801 ccaaatatta aaaaggcact tcttgaacga gaatatgaag gtaatataag ggaattggaa
    19861 ggtatgatgg aaagggctgt tattgtggaa tcatttgact cgattttatt agaaaaggtt
    19921 agcaatgaat atacaaataa tatagttgat aaagataata gtgattttga atgcagtaat
    19981 ttagatttaa aaactttaga aaaaaaatat atctataaaa tttataacga aaataataga
    20041 aatactacaa aaacctgtga aattctaaag ataactcgct ccactctatg gcgtaagtta
    20101 aaagaaatag aagatgatgt ttaaaaataa tacataccta tatatatcaa aacgttttat
    20161 gtttaatttt aatacataag gcgtttattt gttttcaata aaaaatacaa ttatatgata
    20221 aaaagaatct ttgtaagtga aattctcatc aatcatacct ttaattataa gtaatctaca
    20281 gcatttcaat cgattattaa aaataatcat gataactaaa aatacaaact ctatatattg
    20341 gcatagtaat tgcttttata atacctatat aaaaatgaaa gggagtgata ctagatgcac
    20401 atcttaaaat cagtgaaaaa gataccagga ggacttatgg tctgtccctt gttattggga
    20461 gcactattta atacagtatg tccacaagct cttgaaattg gaggattcac aacatcctta
    20521 tttaaaactg gtgcaatgag tatacttgct ctattttgtc tatgcaatgg agctcaaatc
    20581 aatatcaggc aagctggtaa gccattgact aaaggtatag tacttacagc aacaaaattt
    20641 ataattggtg cagtattagg tattattgtt agtaaattta ttggacctaa aggtataatt
    20701 ggtattacac ctcttgccat agttgctacc atgactaatt caaatggagg tctatttgct
    20761 gctcttgcag gtgaatatgg tgactccaca gatgttggag caatatctat cttatctcta
    20821 aatgatggtc ctttttttac aatgcttgct tttggagtaa ctggacttgc aaatgtacct
    20881 atagtagcac tatttgcagc acttatacca atactacttg gatttatact tggtaacctt
    20941 gatgaagata tacgagaatt tcttgcacct ggtacaacat tattaattcc tttcttcgct
    21001 tttcctttag gagcagcatt aaactttaat caaattataa ctgctggact acctggagtt
    21061 gtacttggtt taggagttac tataataaca ggtatgggtg gctactttgt aatgaaactt
    21121 atacgtgcag aacatccagc agtaggtgca gctacaggta caacagccgg aaatgcagct
    21181 ggtactcctg aagctttagc agctatagac cctactctag ctacaatagc agctgtttca
    21241 acagtacagg ttgctgctgc tattattgtt acagcaatat gttgtccaat gttagtttcc
    21301 tatctagata aaagagaaaa aaagaaaaaa tctattttta aaacaaataa aaatattgaa
    21361 gtaaattaat acttaaggag gtaaaattaa tgaatgcaat tattcatgca aatgaaaaag
    21421 ataatctagt tacatgtgtc agacctttag ttaaaggtga aaaagtttca attgatggaa
    21481 agtcttacat agtaaatgat aatatacctg tctttcataa gatggctaca gaagatgtaa
    21541 aaaaaggtga tgttgtatac aaatacggtg aaattatagg aattgcaaca gttgacataa
    21601 aaactggtga ctatgttcat gtacataaca ttgaaagtac acgtggtcgt ggagataaaa
    21661 aataggagga agttaaaatg aaattaatgg gttatttaag agaaaatggt cagtttggta
    21721 ttcgtaatca tgttttaatt attccaacat ctgtatgttc aagtgagaca gctacaagaa
    21781 tagcatcact tgtgcctgga gcaatagcaa tccctcatca acatggatgt tgtcaaattg
    21841 gttctgatat agagcttaca gcaaagacat taataggatt tggaaaaaat cctaatgttg
    21901 ccgcagtttt agtagttggt cttggatgtg atggtattca agcaaaagaa ttagcatctg
    21961 aaatagctac aacaggaaaa aaggtagatt acgttgttat acaagaatgt ggtggaacat
    22021 taaaaacagt ttctaaggga actgaaatag taagtaaaat ggctcgtgaa gtatcaaaag
    22081 aagtaagagt tgaatttggt atgagtgaaa ttacattagc acttgaatgt ggtggttctg
    22141 acccaacaag tggaatagct tcaaaccctt caataggcgt tgcatcaaac cttcttgtag
    22201 atgaaggtgg aagtagcata ctaagtgaga ctacagaagt aattggtgca gaacatttgt
    22261 tggctacccg ttttgaagat gaagaaatga aagataagtt tcttaaattt gtaagtgatg
    22321 tagaaaaaag agcaattgct atgggagaag atttacgtag tggtcaacct actccaggta
    22381 ataaagctgg tggactttca acaattgaag aaaaatcact tgggtgtatg tataaggcag
    22441 gcaataagcc attcaaaggt gctcttgaat atgctgatat tgtacctcct gataaaaaag
    22501 gattatattt tatggatact ccaggtcaag atatagattc tatcactggt atggtagcag
    22561 gtggtgcaca aattgtaatc ttttcaactg gtagaggtac tccaacagga agccctattt
    22621 caccagttat taaaatcact gggaatagtg atacatacaa caaaatgcca gacaatatcg
    22681 atataaatgc aggtagaatt ataacagatg gtgctaaaat agcagatata ggtcaagaaa
    22741 tatttaatga aataatagaa gtctgtaatg gtaaacatac caaagctgaa agtcttggtc
    22801 atagagaatt tggcatttac agaatttcat ctacatttta gattgtaaca aaatatcttt
    22861 tataaatata atacttatgt agatacataa gctaaaagtt ctttctaatc ctataaaaat
    22921 tttctatact gaaaaggaac tgtatcaaaa ttattttgaa aacagttcct tttttaacct
    22981 cttttactca aaatattata aaataagatt taaacaataa acgtgtcttg attaatctct
    23041 aagaagacat aaataaagct aaatgtaaag tgagataaat aaaatttaat atctttgtat
    23101 gaaaagatat ttggtataat atgtctgaga gaattcttat ttgtaaacaa ataaaggatg
    23161 gaggatgaaa caaaagaatg aactttgaaa acacaaaaaa taaaaaaata atagctactt
    23221 tactattgac actcctttta atcgtgtcat taacatgtgt caattttatt atgaatctaa
    23281 ataaagtaac acataatgaa acagaacgat atcttgtaga agtttcacaa agtatttcca
    23341 ctacaattaa tgtgaaatgt aaaagtatat ttaggacact atattctatg ggagatactt
    23401 attttgagat taatgatagt gaaaaagaag tcaatagata cttatcaaaa aaattaaaat
    23461 tatttgaatt tgattggtta ggtattatag attctaatgg aaattttgta agtgccctta
    23521 aagatacagg taatgttaaa ggacaagact tttatagaaa agcccttcaa ggtaaagaaa
    23581 caattactcc tgttaaatca caatctggta catatggaat tttatattct gtaccactat
    23641 acaatggcga taaaattgtt ggagctgttg ctgcttggaa tactattgat actatgagaa
    23701 atttattaag tgtagaaagc tttcatggtg aaggattttc ccaaattatc gaccaagatg
    23761 gaaattttat tgtaagttca gcaaacaaaa atgctccaaa aaatgtggta aatttttttg
    23821 acatgattaa acaccaagga aaacaggttg ataaaaataa acttgaagaa atgaaaataa
    23881 acttaaaaaa gaacaaaact ggttctatgt attatacatt aaatgatggt atttctaagg
    23941 caatgcatta tcttccatta caagaagaaa acttatacct cctttcagtt gttccaacaa
    24001 aaacagcaag tgcacagttt gatttacttc taaaaaaagc aatcttcatc aatattttaa
    24061 ttgttatact atttttattg cttattatat tgattatgtt tataaatcaa aagaacaggg
    24121 cacaacttat aaaaatcgcc tatgtagact ctgtaactaa aggttacagt aaagcacgtt
    24181 ttgatattga agctaaaaaa attatacagt catcttcatc agaaagctat tcattggtat
    24241 ctataaatat tcagaaattc aaacttatta atgattcttt tggtagtgaa gcaggagata
    24301 aaacattaaa gtacatacat gatgtcatta gtaattatct agaaccagga gaattgcttt
    24361 gtcgagtttc taatgaccag tttaatgtac ttattaaaac tattcctcaa aaagaaattc
    24421 ttaagtacat agaaaatatc accaatgata ttaatagttt caataaaaat gaagaatgta
    24481 aatattttat agctctatct ataggtgtat ataaaattga tgacccaaat ctacctttag
    24541 tttcaattca agaccgtgca aatgttgcac gaaaaaataa taaaggaaac attggaaatc
    24601 atccacttta tacatgtgtt ttttatagtg atttagagcg tttaaaaatg attaaagaaa
    24661 aagaaatgga aaatagaatg gaggaagcat taaataataa cgaatttata gtatatcttc
    24721 agcctaaaat aagcctcaaa gataatacta ttgtaggtgc agaagcatta gtaagatggc
    24781 aagacccaaa taaaggcctt ataccaccag acgagtttat tcctttcttt gaaaaaaatg
    24841 gttttattac aaaactcgac cactatgttt ttgaaaaagt atgtagtatg ataagaaaat
    24901 ggatagataa tggagaaact ccaatgcctg tttcagttaa tttttcacgt gctaatttaa
    24961 ataatataga ttttttagaa aaatacagaa tgcttcgtaa taaatacaac attcctgcta
    25021 agctcttaga aattgaatta actgaaacac ttgtatttga aaatctccaa aaattaatta
    25081 atataataga ccaaatccat gaagaaggtt ttcaatgttc actagatgac tttggaagtg
    25141 gatactcttc actcaatatg ttaaaagaaa taaaagttga cacattaaaa cttgaccgtg
    25201 ctttttttag ctcacctaat gcagataata ttgctgaaaa tcatgtaata gaatctatag
    25261 ttaatttggc aaaaaaatta aatatgaata gtatatcaga aggtgttgaa acaatcttgc
    25321 aaatggatta tcttaaaaaa attaattgtg atatgataca aggatatgtt ttttcaaaac
    25381 ctctgcctca agaaatattt gagaaaatga cttttggaaa aaaactagaa gaaaccagta
    25441 tggatagttc cgtttaaata aaataaaaaa cttgtctcta taaaataatt ttaatcattt
    25501 tataaagaca agtcttttta ttttccaatt taattttatt ttctaaattt cttatactcg
    25561 gcttgtttta aattcccttt ttaagaataa taatacgatt atcatagcaa taaaatctgc
    25621 aactggctgt gccaaccaaa caccatctaa attaaattga attggaagta caatgattaa
    25681 tggtataaat aaaataaatt gtcttaaaag agttaaaaac attgaatgtt taactttact
    25741 tatacactga aaatacacag gtcctacaat agaaatcccc attataggca atgtaattag
    25801 atttatgctt atacctctta ctgctatatc catcaaatct gaatctttat taaatatacc
    25861 tacaaatact tctgggaaaa cttgtactat taaaaatcca aatgacaata tgacaactga
    25921 cactatgata gataatatta aagttttctt tgcacgttca tattgttttg caccataatt
    25981 gtatgatatt atagtttgca taccttgatt caatccaaac acaggcatca taaacatcaa
    26041 taaaatagat gtaagtgctg tcatagcccc aatagctaaa tctccaccat aaacctttag
    26101 agaattgttt gtaaacatgt gagttatact tatagctact tccatagcaa aaggtgtcat
    26161 tcctattgca aataccttct ttactatatc tttattcaat tttaagttac atttttttag
    26221 ttttaaagtg gacttccctc ttataaaata atatgttacc caaaagaata ctactatttg
    26281 acatagtact gttgctatag ctgctccttt tatacctaaa ttaaacacaa atataaatat
    26341 agggtccaat atcaggttta atacacatcc tacaatcatt gtccttgctg ctaattttgg
    26401 attaccttct gctctaattg cattattaag agaaaatgca actaaattaa atatacttcc
    26461 aatcaatatg acactcatat agtcttttgc ataaaatatt gaatctttac ttgcacccaa
    26521 aataaaaagt atatcttcta agaaaactaa tcctaatata gttataatta aagagataat
    26581 tatagataat gtcaaagcat tacctaatat cttctctgct tcttctctat ttctttcccc
    26641 taattttata gacaatcttg tgctagcacc tactgatacc aacattccaa aagctattat
    26701 tagagtaaat actggcatag ttacaccaag acctgcaata gctatagagc caataccttc
    26761 catagaacca ataaaagctc tatctactac attataaagt gaagttacca tcattgctat
    26821 tattgccggt atcgaatacc taaataataa aatccatatt ttttcatgtc ttaatgcttc
    26881 ttgattctcc atgttgtcac catttacacc atgtcataag ttatacaaat tggtttattt
    26941 gttcttgggt ctgttgctat ttctgcatca atattaaata attcttttag attatccttc
    27001 gtcataacat catatgcatc accttcacaa actattttac ctttcttaat acctatcatg
    27061 tggtcagcaa atcttgctgc attattaagt tcatgtatta ccatgacaat agttcttcct
    27121 tgttttttat ttagttcatc taataatttt agtatctcta attgatgtgc caaatctaaa
    27181 tatgttgtag gctcatccag taataatata tcagtttctt gtgccaaagc catagctatc
    27241 catgcccttt gtctttgtcc tcctgataaa tcttccatat ttctatcctt aaattctgca
    27301 attccagtaa cttctaaact ccaatttaca atctctctgt cttctttact agaattacca
    27361 aatccacttt tatgaggaaa tcttccataa gaaattaatt ctgaaacggt aagaccacta
    27421 ggagcttgag ggctttgtgg aagtacagcc atatcctttg ctatttcttt agacttcttt
    27481 ttgtagatgt cctctccatt tatatatatt ttcccactat tagggctaat aattctacct
    27541 atagttttaa gtattgtcga ttttccacaa ccattagccc ctatgatggt agttattttt
    27601 ccttttggta tatttaaatt taaatttttt ataatatctg tgtttccata tgatacattt
    27661 agattcttag tatttataca gttcataaca acaccctttc ctaatcattt accaacatta
    27721 aataaataaa gtacggcact cctattatag aaactactat acctactggt atttctatag
    27781 gtgctagaag atttcttgat atagtatctg ctattaaaag taataatgtt cctatcaatg
    27841 ctgatactgg tattaatctt ttatgctttg gtcctattat ttttctacct atatgtggag
    27901 ctattaaacc taaaaatgaa atactacctg ctactgctgt cgatacacca gataaaatta
    27961 ctgcatatat tataagtctt cttctttgtt tttctacatc tacaccaaga cttatagata
    28021 cttcatcacc caaatttaaa acatctatat atttagattt ataaatagtt atacccataa
    28081 aaatcactat aaatggaagc acagctatta catatttcca actggttccc caaatgcttc
    28141 ctgatgtcca tgccataacc ctattaaatt cttgtgttgt aaatttcaat tggaatatcg
    28201 tcaaaattga agtaaaagct atgtttacac ctataccaat aagtagtaat cttgacgaat
    28261 ttataccacc tttccatgca agagaatata taagaaatgc tccaaataaa gctcctataa
    28321 gtgcaacaat aggcattgta aatacagtca agttactcat accttcatat acattaccat
    28381 tcataaggta tatataaact actacaaaca atgcagcacc tgaatttatt cccaaaattc
    28441 cagaatcagc taaatcattc tttgtaactc cttgaagtat tacacctgct gttgataatg
    28501 cagaaccaac caaaagtgct attactattc ttggtaatct taatttaaat attgcaattt
    28561 catgattacg ctgaccctgc cctaaaaaag tctttataac atccattggt tctatagaaa
    28621 atgagcccat atttaaactc aataaaaaag tgataaaaat aagcaccatt aaaattgata
    28681 taacaagtaa tgttttagaa tttattttta taatattttt catagtacct atttctcccc
    28741 ctttctaaca aggtatataa atattggcac tcctataagt gctgttatag aacctactgg
    28801 tgtctcatat ggaggattta tcatcctact taatatatct gtatatacaa gcaatacagc
    28861 tcctaatact aaagagctcg gtattatata cttataatct gaacctacta ttcctttagc
    28921 tatttgagga actataagac ctataaaaat aatattacca gacactgaca cagatgctcc
    28981 agtcattaat attacaagta ctatacatat aaatctaaca aagttagttt tttgtcctaa
    29041 tcctattgcc acttcttctc caagacttaa tatagttatt cttggagcca taataatagc
    29101 taaaataaat ccaataccac cagctacaaa tagcaatttt ataccttccc acttcactcc
    29161 tacaagccct cctgatatcc agaaacttaa ttgttgagat aggttaaaat acatagatat
    29221 tgctgatgcc aaagaaatta gcaatgtacc aagagcgact cctgcaagag ctaattttac
    29281 attattaact cttcctcttg atttagaact tataaaatat ataaataatc cacttatact
    29341 tgctccaata aatgcaaaca tcattacact aaaacttcca tgtataagtt gaggaagttt
    29401 tttttgaagt accagtgata tcgaaattgc gaatgtagct ccttgtgtaa ttcctataac
    29461 agatggttca gctataggat ttctcgttat tccttgcatt attgccccag aaactgctaa
    29521 aaaccctcca acaagtgcag ctgctattgc tcttggaagt ctaacatcct ttactattct
    29581 catatttatt ccatcttcca tttttatcaa actatctatg acagttgata gattcatatc
    29641 ttttgcccca atagttatgg ataatacaat tccaaataca agtacaacta tacttactaa
    29701 tatcaacaaa taagttatat tttttttact cagcttagtc aattatccta cctccttttc
    29761 ccttttaatc gtcagtataa tatcatctat taagtatttt aattttaaga tttattaaaa
    29821 ataggtgtga aaaatgaata tcattatctc acacctacta agttaagaat tactttgtta
    29881 attcattttt aactgattct aataataact cttttccaat tggattgtat gattgactga
    29941 aaaatggagc agcatcaagt attgttacat taccatcttt tactgctctt atttgagacc
    30001 agatagcact attttctaaa tcttttttat ctgcttctgt agcaattaca actatatggt
    30061 ctgcatctat atctgttaaa ccttccatac ttaccattgg tagtgttatt ccatcctgtt
    30121 ttggcatatt cttaggtcta gctaatttca tatcatcatt gattagagta cctattccac
    30181 catcactaaa taccataaat tgacctgaac ttgccaacac tggtaaataa gttttttctc
    30241 cattcttttc tataacttcc ttacctaact ttgtagcttt ttcatcatac ttctgtagcc
    30301 aacttttagc ttcttcttct ttgtcaaata actttgaaac atcagttaac ttgcttctcc
    30361 agtcatttgc ataatctttc atcataacta caggagctat ttcttttaat tggtcatata
    30421 ttttttcttg tctttgactc attattatta aatcaggatt tacttctaat atagcttcca
    30481 tatccatagt atccatcatt gagtgcccaa ctatctttac acctttaagc tcttctctta
    30541 tgtaacttgg taatttatca gtttcatatg agtctacatt tgctgttgca acaggcttat
    30601 aacctgctaa taatagttct tcactacttc ctgatatatc aactattttt ttaggatttg
    30661 atggtatctt aacctctcct ttaacagatt gtacaactct tgtctctgat ttatctttat
    30721 cctctgtatt cttatctgaa catgctgtaa caagtactaa cgtagttata atagatataa
    30781 atattgcaag tgattttatt tttttcatga ttacttctcc tacttgttta ttatacttac
    30841 atgatattga ttatcataag cacatattta ttatagcatt tttctatttt tagtcaatat
    30901 tattttgtaa aaggataaaa attcctaatt aatcttaatt atatatagct tagataaggt
    30961 tttcttatat taaacagtat tttttttaag aattggacac aaacacaatg acttttaggt
    31021 taaacaatga taaaatgcaa tttatagata atgaatttag taaggaggat tcaaaacatg
    31081 gtacatttag tatattgtga taatgcagga aaaaaaggag aaaaggtctt agataaaatc
    31141 ttggctggaa caaaaacaat ggttattaga ggtgctgctg gtagaaaaat accacatagt
    31201 agagtatttg aagatgagtt actttatttt atggaaaaag gaactggaaa aatatctgca
    31261 aaagccactg ttaaaaaagt agaaaactat gttaaattaa cagaagatga aatttctaaa
    31321 attttaaatg aaaatcaacc aaaattaaat ttatctgaaa agcaaaaagt tcgttggcat
    31381 aaaaaatgtt tatgcttagt tgaatttgaa aatgttgaag aaataacacc tcttgatttt
    31441 gaccatcaag gaaatatgga tgattggctt attgttgaaa aaattgaaga tgtagttgtt
    31501 ggtactagta taccttacaa ttatgagaag tctaaatttt aaagtatcaa taacataata
    31561 ctatattgct aaactctact taaataaaaa aagagtgcat ttaataatta taaaaattat
    31621 ttttatacac tcttttttaa taataaattt aaattactta ctttctaatc tttcatatac
    31681 atctataaat tcttctgcta attgttgaaa attcatagct gtcattaaat ggtcttgtgc
    31741 atgaataagt ataacactta tttcagtttt attaccatta gcttcatttt gtatcaattc
    31801 tgtttggaat ttatgagctt tatgaattga ttctttcgac ttttcaatac attctctagc
    31861 tttatctata ttcccgctct ttgcttcttt tattgcttca aatgctagcc ctttcgcatc
    31921 acctgcaaat ccaattattc caaaagatat ctctatcatc ttctcatcca tttattcgac
    31981 ctcctaaata tctctatagc ttgatatgac taatccattc ttttctaaaa attctttgac
    32041 ttttttagat gtaagtatct tgtgctcttt cgttctatca atagcataag atgtagaatt
    32101 taaaatataa tcatctaaaa aagctgggtg agacataata tccactacat catacttcat
    32161 tatttcgtct atatttttta taaaatattc ttcacttaca ttttccttat aaaaactatc
    32221 tattaaagga actactttag aatactctaa attatattca aatccacctc ttattggaag
    32281 attatacttg gttacaatct tctctactac tggctttaaa cttactaaag tatggatatg
    32341 atggtgacta tctaaatgag atatatctat tccaagccct tttaccctat ctatttgagc
    32401 acaaagttct ctataaatct catcacaatc cattttttca ataatttcgt ctgtaatcct
    32461 cctaataaaa aaaccgtctt tatcaacaat agtttttaag ttagataata atggtctacc
    32521 acaacttaaa gtcatatgaa ctccacaatt taaagtttta ttttctttaa gtaagcctac
    32581 tccatgttct accccaggca tatttgccat catagaagta gacctaacta taccattatt
    32641 atgagctgaa attattccat agttcacagc ttcacaatat ccaaaatcat ctgcatttat
    32701 tataatttta gtcataccat aacctacttt ctatagacaa ctatctatta aatctaggaa
    32761 gataatcttt atgagcttcc aacatttcgt ctaatatagt ttttgctaag tcatcagatg
    32821 gaatcaatgg gtttatacaa agtgccaaaa gagcagattc ataatcgcca tatacagctg
    32881 ctttagctac caatacttca aatgatttga tttggcttac taatccatgc acagaatctg
    32941 gtaaatatcc tatacttatt ggttttggac catttttggt tattacacta ctcacttcta
    33001 ccgcttgttc atctttaaaa tctcttatag aaccattgtt taatgtatta actacttgga
    33061 tatctttttt gtcattgtat atagaactta ttaaattaca agcagcatca ctatagtaag
    33121 cccctcctct tttttcaagt tgaggtggtt ttacatctaa tttttcatcc ttatataatt
    33181 caaataattg ttcttccaat tctttaacta cttgacctct agcttttccc tctttaaact
    33241 ctctcaactc atcctcaagc atttctttag ttttatagta gtatctatga tatggacaag
    33301 gtattgcacc caaagatttt acaaattcag cattaaaatc aattgccttt atattttgca
    33361 tacttatatc tgctttgaca aacttatcaa ttgcttcttt tgtaacatct tctccatcta
    33421 aagatacatt tagaccataa accatatgat taagacctgc aaaatccata cttattctgt
    33481 cactttctac attaaatagt ttggcaacat cattttttaa atgaattggc acattgcata
    33541 gacctatata tcttctaaaa ttagtatatt tgaatacagc ctctgttatt attcctgttg
    33601 gatttgtaaa attcaccatc catgcatttg gacataattc ttccacatct tttattatat
    33661 caaatataac tggtattgtg cgaagggctt taaataaacc tccagcacca ttagtttcct
    33721 gccccataac tccatgagat aaaggaatag tttcatcttt tattctcgca tctaaaagac
    33781 ctactctaag ttgtgttgtc acaaagtctg catctcttag agcttctctt ctatctaaag
    33841 taagatttat tttcatatct actccagctt tttttaccat cctcttagct agatttccaa
    33901 ctatctctaa tttttctttt ccagcttcta tatctacaag ccataattcc tttacaggta
    33961 actcttcata tctatttata aaaccctcta caagctcagg agtataactt gaacctcctc
    34021 caattgtaac tatttttagt ccctttttca tctcattata ccatccttac ctataattta
    34081 atatactcta caaaacccta tacatttatt taccgaaata aaactaccta gttgttaatt
    34141 ttcaatttat tagcttccat tttttcagac gctatgacaa atggaatata tattaatatt
    34201 gataatatta aattaaatag tgctaaagct ccaccagtcc agtcacctgt agccatcgct
    34261 ccacctaata taggaggtgt tacccaaggt attttaggta aaattactgg tgctactaat
    34321 ccaaaatcta ttgctaagta tgatacagca ctacatatca ctggtgcaag tacaaatgga
    34381 atcataaata taggatttaa aactattgga agtccgaata tcaatggttc tcctatatta
    34441 aatattgcag gtggcccacc taatactatc atttctttac gtcttctccc agctattaat
    34501 aaagctacta ttaatcccaa taccattcca gaaccaccta aatatacaaa tgcatcaaag
    34561 aatgcacctg caagtacatg gtatccttcc gttgctcctg cttgagccaa ggcagcattt
    34621 tctccaccta atttcataag tatagtttct gtaaaaggaa gtgctatatt tgcaccatga
    34681 agtccaacag tccaaagtat atgaattata aatgcaataa gcattgtaga acctagacta
    34741 tcagctacat ttgatagcgg tgctcctaaa taagtattta aaatatctgt taagaaactt
    34801 ccattagaaa gcatttttat aaatattcct attacagtaa atatcattat agttaacatt
    34861 cctggtatta actttgcaaa tgatcttgaa actgctggtg gaactccatc tggcattttt
    34921 atagttaatt tctttacctt agataatctt acaaatatct cagttgataa taatcctatt
    34981 aatatagaag agaataaagc tgctgtacct aagtaggttt gttgtatcat tccccatcct
    35041 tcaactactg tagttcctcc ctcaggaact attttaccaa tttgtggagc cattataaaa
    35101 aagatagata aagctataag cccagattgt aacccatcac tctcataaga ttttgctaga
    35161 aaataagcaa cacctattac taagaatact gccattgtac ctattgctcc ccaccaaagg
    35221 tcgcctccaa atgtggtcca attttctcca aatatacttg ccatcaaatt tttgtatgct
    35281 tccaatggta agttatttat caatgttccc aaggcaccta ccatagttat aggtatcatt
    35341 actataaatg catctcttac tgctactaag tgcctttgag ctcctatttt agcagctaca
    35401 ggcactatat atttatccat aaatgacatg aatttttcca ttttacatcc tccagtacaa
    35461 ctttattttg aactatttaa ctctacagct ctatctaaca cagcttttcc attacatctt
    35521 ccataatcca tcatatttat aacttctact ggaatattat atggttttgc catttcagtt
    35581 gcttttccta atacatatct aacttgaggc cctaaaagta agacatcaca attttctatt
    35641 tcatttttta agttcacttc tgatatagcc catatttttg cttctatacc gttttccttt
    35701 gctgcatcct ccatttttgt aactaataaa ctagttgaca ttcctgctga acaagctaac
    35761 attattttta tcattttttc ccctccaaat tagattcatt aaatattttt taagtcataa
    35821 attttttaaa ctatacattt aaaatatgct tttctattaa ttttttctta atgtatatat
    35881 cttattttta aatgtatata catttaattc ttgatattat tatataccaa atgtatatac
    35941 atttcaagaa aactttttca ttttatttaa attttattcg acaacagcat tcaaagttaa
    36001 tgtatatcca tagtgatatg tgtgtgagta ctcaaaaatc tcactatttt gtaacttaca
    36061 aatttggctt atatataaaa gtggctctgg ttcttctaat tttagattat ccatacattc
    36121 ttcattcgat attacagctt gtacttctct ctctgacatg gctatcttgt atccacacac
    36181 ttcttcaaca aaattataaa gagaattttc acaatgagtc ttatttaaat ttggaaataa
    36241 cttaactggc atataagtaa tcatatatga aactcttgca tcattggcta atcttactct
    36301 ttttatttcc caaacatttt cttctaaact tatattcaac ttacttgata aattttcatc
    36361 tgcctgaact atttcaagag ataccaatat attagatact ttatagcctt tactttccat
    36421 ctctttagta aatccactta cagacgatat attatcatat atattatttg caataactat
    36481 actacctaca cctctttgtc tagttatata gccttcttta actagattat taagtgcaga
    36541 ccttacagtc attctagtta cattaaattc ttcactcagc tgtttttcag aagggattaa
    36601 atccccttct ttcaaactgt ctgaatttat taactctcta acatgatttt ctataacttt
    36661 ataaattggc tctttcattt aattcactcc ttattaatta aatcttttat ataataagat
    36721 aattttatat acaaataaag aaaatgtcta tacatttaag ataatttaaa ttttattcag
    36781 acatcttgta caactttccg agttttaata aagatataaa tagacttatc attaatatac
    36841 cttttaatat acttgataag gttatactcc accatacacc atttagaccc aacatttctg
    36901 gtctagatat aaagtaagcc aatggtactc ttgcaccagt cagtatagta attataatag
    36961 aaggtatata ggttctgcct aatcctttga aagctcctgt tgttattatt tctaaacaca
    37021 taaaaaattg agaatagcct aatattttaa gataaattgc tcctttttct attgcctcac
    37081 tttcatctat aaaaatagaa aatatactct ctcctgcaaa tactaatacc aaagtagtta
    37141 ttatcccaag tacagaagat acagttatag ctattttgca acctttattt attctagaat
    37201 acttcttggc accataattt tgtccaacaa aactgcctaa tgcaactgat agcccttcag
    37261 ccgtcatcca ggaaattgct tctatttgtg agcctacttt ttgtactgca acagccacag
    37321 gcccccatga tgctactatt actcctaata gcattgagaa tactgtaaac ataccacttt
    37381 gtattgctat aggtaatccc aatttataca gaactttata atatttagtc tctatttctt
    37441 taaatagtct taatttaaaa tattcttctt tacttttaac tattatatat ataaaacatg
    37501 tagtaactac tatctgagca aatacagttg caatagcagc tcctgcaaca ccaagttttg
    37561 gagcaaatcc ccatccaaaa attaatatag gatcaagtat aatattagtt attaatccta
    37621 ctgtattaat acgaaaagga gttttactat ttccaagacc gttgaaaatt gcagtaaata
    37681 ctggattaat aaaataaaat accataccta gagctactat tatcaaatac tgtctagaca
    37741 tggttataac ttctaagtct cctaatctaa aaaaacctat caattgttta ttaagtacta
    37801 ttaaagaaat tgtatatatt attgctagta taatatttat ttctattgct gacttcatat
    37861 atgatttagt agtgcttata ttatttgcac caatactttg tgctactttt atctctccac
    37921 ctacttttga aatcataata aatgccattg caagccatgg aaaaaaacca gctgttccaa
    37981 ctgcagctac tgctttactt ccggctttac caacccatat catatctatc atgttatacc
    38041 ccatctgtat aaatgaagtc cccattattg gaagtgatag cttaagtaat ttttctgtaa
    38101 ttcttccttc tgttaaatta attctgtctt tcattttcca cctcttttat attaaattta
    38161 tttgcttata tagctagatt aatcatttta tttgattgag ttcactatcc ttcaatttat
    38221 attatcatat ctcaagattg catctcaacc atacttatgt ttaagataaa gtattttacc
    38281 tatctctgat aaaattaata cttatgaata taaatatagt catttcatca atacatatta
    38341 taacccttaa cttaacttca attaagtata catactgtaa tcaattagaa tattttataa
    38401 tgtatcttcc attaagaaat aacaaatata agtgatttat ctataatttt atttatacat
    38461 aaaatagtat actgcaataa taaaaaaact atatttgaat actttaagca tgtttactaa
    38521 ttgtaatttc cacacaaatt tgataatata tgaatatatt atggtgaaat ttatagatat
    38581 atattaatta tggggagatt gtgggaggtt cttttcacaa gacatttaag ctatgaaaat
    38641 aaataaaaaa tttaatagag ttgatatgca ggtttcaata cttactgcaa ttattgtaat
    38701 aatatcttcc tcatgtgtat atttttttaa ttatacatta acttataaga gcatgatata
    38761 ctctttaagt gagaggtcaa aacatatcta tgaatatgta gagagaaata ttgataaaaa
    38821 tacctttact gaaattaatt ctaaagaaga ccaatcaaaa aagtcttata aaagttctaa
    38881 aatcctatta gaatctgtaa aaaatactac tggagtcatg tatctatata ctgctaaaaa
    38941 aacaaaagat ggatcactag tatatgttgt tgatggttta aattcatcta gaagtgattt
    39001 tagaaatgct ggtgatttga tagaaaaaga gatttgggga gatcttaaca aagcattaga
    39061 taatcatata ttgttaccta aaaagattaa atcaacatct tggggataca tatttatatc
    39121 gtattttcct attcacaatg atgttggtaa ggttgttgga gtaataggaa ttgaatttga
    39181 agcaaaacat caatacaata catttaaatt tataagtata gttacaccaa taatagcatt
    39241 attaacttgt ctaatatcag cattgattgc agtgattcta tttaaaagaa tatctaatcc
    39301 tacatatact gattttgcta atacagatta tttaacaggc ctaaaaaata gaaatgcttt
    39361 tgaaatagat atgaataact taaacaattc aaatctaaag aatctaatat ctatcatatc
    39421 aatagattta gacggtttaa aaaagattaa tgataaattt ggccatcaga caggagactt
    39481 atatattaag caagcaagta aaattataca agatgtaata aataaaaaat atatagttta
    39541 tcgtattgga ggtgatgaat acataattat tctaaaaaat ttatctcaca aagaaataaa
    39601 tagtattata aataatatta atttaaaaat agttgaagaa aactcaacta atgaattaaa
    39661 attatcaatg tctattggat atgctatttt tgatgaaaaa gtagatgttt ctttatttga
    39721 tacatatcgt cgtgcagatt ctcaaatgta tgataacaaa aagaaaatta ataaataata
    39781 tcaataataa atttaaaaag tctattcttt aaataataaa tcataagaat agacttttta
    39841 aatttgtgtt tttataagat aaattattta attaaagttt gttctttttt ctaaacgatt
    39901 ctttataaat tcatatatta ctttcactaa tatatttgtg actaatatta acacagctag
    39961 tgctgctgcc tgtgcagtac tccccttatc atctagttgt acaattgata ttgcagctac
    40021 ttttgaactt gcagtatata taaaaactag tgctgatatt gtcaccatag agtttagaaa
    40081 aaagtacata aagttctcta aaactgctgg caaacaaagt ggtatagtta cgttcttaag
    40141 aattgatgcg ctgtttattc ccattgattt agctaccatg tccaattctt tatctacttt
    40201 cttaatagca gtagtagctg ttataaatgc tactgaaaag aaatgaacta tattacatat
    40261 aaccattata agtaatgttc catataatcc attaaacaaa tttttaatgt atattgtatc
    40321 cataaaatta aactctaatt tattaaaaaa taatacataa gctataccta aaactaatcc
    40381 tggcaatgcc ataggcaatg tacttaaaaa atacccaaat gattttaaat atggagcttt
    40441 ttctgttttt tcatttaaat atgcaaataa gaaaacaaag ctcgtcccta gaatagctgt
    40501 caatagtgac atttttattg aatttataaa tggtttaatt ccatcaatac tgtttttcat
    40561 agaaaagtgt tccagtgtaa gtcttaaatc atatggccaa actgatatta atgaggataa
    40621 gattacagtt aacattacag ctattatacc aaatgcaata attcctacaa cccctcctaa
    40681 aatactatct cttaacttat tagttttaac cttatatgga attgactttg aagttatata
    40741 tgagttttga tttttttcaa tttttttagt tactataaac gatattattg caggtattaa
    40801 aagtaatatt gatacagttg ctcccattgg catattctgt tgccctataa tttgcttata
    40861 tacatctatt gataatacat taaactctcc tcctatcacc tttggagctc caaaatctgt
    40921 aaaacataat gtaaatgata cgaatgcaga acttattaaa gagtatttta tacttgggat
    40981 tgttatgctt ataaattctc ttatcttgct tatccccata gtattactag cttcatatag
    41041 tctataatcg gttgaactta gagatatata aagtatttga aaggattgag gaaatacata
    41101 tataatttgt gccaatatta ttcccactgc tccatatatt ggaacatgca ctccaagtaa
    41161 attagttacc aaaccttgat ttccaaataa atatactaga cctattgcat gcatcattgt
    41221 tggagcaaac ataggaaaca tagctaatga tttaaaaaaa cctttaaatt taatctttgt
    41281 tctagttaat gcatatgcat atactaatgc aagtgttagt gatattacag ttgatataac
    41341 tgatacaaat actgtatttt ttagagaatt taatagagat tcagaagcca agtattcttg
    41401 aaagttttgt aatcctacaa agactccatc tttatctaaa aaagcctgta caaatagata
    41461 tattataggt gctaataaaa atattactaa agacataact aatactgtaa gcatacattt
    41521 ttttatatct aaattcattt ttgatttaag acttatactt ttagaactta tagcattatt
    41581 attcatacac ttatgctcct ttaacttttg gatactttaa atagtatttt tcgtctatac
    41641 ttatttttac aaggtcacct tctactaaat ttaaattaac ccactctttt atagaaacat
    41701 cacttattat gaaattttca tttaatttat cttttatttc aactctcaat aaattccctc
    41761 taaactctat atttgatatt ataccttggt agttttcttt tgtcgatttc tctatttgaa
    41821 catattctgg tcttacagtt aatatagaat ctccattatc aaaacagttt gtatctccta
    41881 taaattgagc tgtaaatagg ttttgaggat tttggtatat ttcttctgga gtccctattt
    41941 gcattatatc tcctccattt agaatagcta ttttatctgc catagttata gcttcttctt
    42001 ggtcatgtgt taccattatt gtcgttatat taagttcttt ttgtaatctt ttaatgtcca
    42061 ttctaagctt gtgtctaacc tttgcatcta atgcggacat tggttcatct aaaagtaaaa
    42121 actttggttc aagagctaat gctcttgcta ttgcaatcct ctgttgttgc ccacctgata
    42181 atgctttagg atatttatga gcttcattag tcaatccaac tgtttcaagt acttccttaa
    42241 ctttattatc tattttttct tttgaaactt ttctctcttt tagtggaaaa gctatattgt
    42301 tataagcagt catattcgga aataatgcat atgattgaaa tactatacca aaacctctct
    42361 ttgatggctc taaatttgta atatctttat cctggagaat tatagttcca ctatttacat
    42421 cttcaagacc tgctattatt ctaagtaatg tagttttacc acatccacta ggtccgagta
    42481 aacatagaaa ttccccttct tcaatatcca aactaatatt atttaaaacc cttttttggt
    42541 cataactttt aaacacatta tttattttta agtaactcat atcttatctc tccttatata
    42601 gttatttttt tattttaaat tttatatcct actttttctt ctaaaattct ttcatctaaa
    42661 ttattaattt aaaccttact tactttcaga ttttgaaccg tattttgatt cccaagtttt
    42721 aagtactgta cttctgtttt tggcagcaga gtttaaatca acttctttta taagctgttc
    42781 aaatggttgt ttagaataac cttttggaac tggattatat gtctttatgg aagtaactgc
    42841 tacttcatgt gcatatctat ccatagctga atcacttatt gcccaatcta aaaatagctt
    42901 tgcttctttt tttatattat cttttttaac taaggcattt gcttctaagt cccaacctga
    42961 accttccttt ggaaatacaa cctctatagg ttctccacta gataattgtt gtgtagctct
    43021 atatccaagt gtaataccta ttggatactc tccagagcca gctaacttcg caggttttga
    43081 acctgagtgt gtatatgtag ctatattttt atgtaattta tccatgtaat tgtaagcatc
    43141 tttttcaccc atcatttgaa taagccctga tactgtaaga taccccgttc cagatgatgc
    43201 tggatttggc attgttataa gtcctttata ttctggtttt attaaatctt cataactttt
    43261 tggaatatct atacctagtt tcttaagttc cttataatta actactattg ctgtctctgg
    43321 gacactcatt cctacccaag atacatcatc ttcagtatct ttaaattttt tatcaacttt
    43381 atcataattt ttaggcttat atgcttcaag tgcaccatct ttttttagct ctatcatgtt
    43441 cattgctgat aatccccata taatatctgc ttgtggatta tctttttctg ctaatagttt
    43501 tgaagctatt acaccatgag aatccataac tatctctaca tctatattgg gatactcttc
    43561 tctaaaacct tccatataat aagctatctg ctcttcctct agtgctgcat atattgtaag
    43621 tttattattt ccttttcctt gagtagtttg tggtgttgtt gatatgcaac ttgtcataaa
    43681 tgtaatcact acaatgcaca gacaagtaaa tataaattct ttcatatctt ccccctttgt
    43741 gttttttcct ttttttattt gttttttatt gtttttgctt atgtttatat aatacaccta
    43801 atttgttaat ataaagttaa gtgtaaatta acagttcgtt aatttttaga aatttttgtt
    43861 aaatatatta tttttagtta aatacagtct gtttttaaac aatatattaa tttttatagt
    43921 atctacattt taatttaaac ataaaaaata agtctaacct ctataaagta gaatgttaga
    43981 cttattttta taagtattta attatacaac caaatcattt tttttctttt cttcaagtgc
    44041 ttttttaagc accatatcct taaccttcat aagtctagta gtacttgctc tctcattttc
    44101 ttcaagcttc atagctatat acttaatagt ctcaatgtaa tttggtatca ttacattttc
    44161 aagagcatta acacgtctac gtgtcttttc tatctcttgt gcaagaagct gtgcactttt
    44221 ttcactttct gcaagtctta aaagtggttg catagcatct gagaaagctt ccatagcaga
    44281 atctaattca cctgatgtaa aagcaagacc ataaggataa atatcacttt gattgttttc
    44341 agttttaaag tcaaacacag gaacatcaac actcattata tttctcgtag aaacatctac
    44401 tgaaactgat tgctttggca tcataagtgc tgaacccaga tactcctgac tcataactgc
    44461 tcttgcgatt ataaagtttt tataagcagt atcaagagca ttttctgctt cttcacgcaa
    44521 tcgcttattt tctcttacaa tctcaagaaa ttgtttcata agttcatcaa gtttatcttt
    44581 aagaagctta tgacctcttg tagcagtttt aaggagtttc ttaagtctag tcatttccat
    44641 acgtgttgga tttatattta atctagccac agatatcact ccttaggcat atatttttca
    44701 aaatattcat cacggatacg tttaagttca ctttttggta acatactaag aagcttccat
    44761 ccgatttcta atgtctgctc aatagttcta tcagttctaa atccttgtga aacatattct
    44821 ttctcaaatt catctgcaaa cttagcatac attaaatcaa tctcagaaag tgcagattca
    44881 ccaagtatag ccataagttc ttttgcatcc ttacctcttg aataagctgc aaaaagctgg
    44941 ttcattgtat ctgcatggtc ttcacgagtc ttacctttac caataccttt atcttttaga
    45001 cgagaaagtg atggtaaaac atctatagga ggctgtatat cttttttata aagttcacgg
    45061 ctaagtataa tttgcccttc tgtaatgtat cctgtaaggt caggaattgg atgtgtctta
    45121 tcatcctcag gcattgtaag tattggaatc atagttatag acccttcatg ccctttcatt
    45181 tttcctgctc tttcatacat agtagcaagg tcagtataaa gatatcctgg atatccacga
    45241 cgtcctggaa cttctttttt agctgctgaa acttcacgca atgcctctgc atagtttgta
    45301 atatccgtta ttataacaag tacatgcata tcttgctcaa aagcaaggta ttctgctgct
    45361 gtaagtgcca ttctaggtgt agatacacgc tctacagctg ggtcatttgc aaggtttaca
    45421 aacactacag aacggtcgat tgcaccagtt gctttaaaat cgcttataaa aaattcagca
    45481 tcttcaaaag ttataccaac tgcagcaaat acaacggcga acttagaatc agttccacgt
    45541 acttttgcct gtcttgcaat ctgaactgca agattggcat gtggcaaacc agaccctgag
    45601 aatataggaa gtttttgccc tctaaccaat gtattaagtc catctatagc agagactcca
    45661 gtttgtataa attcggccgg ataatcacgt gctgctggat ttattggagc tccatttata
    45721 tcaagtcttt tatctggtat aatttctgga cctccatcaa taggtctacc cattccacta
    45781 aaaacacggc ctaatatatc tggtgaaact ccaaagtcaa gactctttcc taaaaaacga
    45841 actttacttt cagcaaggtt tatacctgtt gagctttcga aaagctgaac aagtgcagta
    45901 tctccattta cctctagaac tttgcaacgg cgaatttctc catttgaaag ttcaatttct
    45961 ccaagttcat caaattttac accttctacg ccgtttatta acataagagg accggctacc
    46021 tcttgtattg acttatattc ctttatcata gttcttcagc ccccctcttt ttaataaggt
    46081 ctgcaagctc gttgtctatt tcatcttcaa ttgaatcata agctgcatct atatcgcctt
    46141 cttctacata cttagcacga ccaatctttt caagaactga aagcttgata atatcgttta
    46201 tagtaacatt ttgcttgata gcatcttgtg ctgacttata gaattttaat ataagtccca
    46261 tcattctata ttgtttatga agtgatgtat aagtatcaac ttcatggaaa gagttttgat
    46321 gaaggaagtc ttctcttata gaacgagtaa cctctagtat caatctatct ccatctgaaa
    46381 gtgaatcaac cccaaccaat tgaactattt cttgaagttc agattcaact tgtaaaagtt
    46441 tcatagcttg agctctttga gctgagaaat tatcatttac atttttatct gcccatacat
    46501 caactttttc ctgatagagt gaataactag tcaaccagtt aattgctggg aaatgacgtt
    46561 tatatgctaa agatgcatca agaccccaga atacttttac tatacgaagc gttgcttggc
    46621 tgacaggttc tgatgtatca ccaccaggag gagaaactgc accaattgct gtaagtgttc
    46681 cttctctttc atccatacct agacaattta cctttcctgc tctctcatag aattgagcaa
    46741 gacgtgaacc taagtatgca ggataacctt cttcaccagg catctcctct aaacgaccac
    46801 tcatctctct aagagcctca gcccatcttg atgtagagtc tgccataagt gcaacactat
    46861 atcccatatc tctaaaatat tcagctattg taataccagt gtatatagaa gcttcacgtg
    46921 cagcaactgg catatctgat gtatttgcta taagcacagt tctttgcata agtgactcgc
    46981 ctgttcttgg gtcttttaat tcaggaaatt caagaagaac atctgtcatt tcatttccac
    47041 gctcgccaca gcctatatat acaactatat ctgcatctgc ccatttagca agctgatgct
    47101 gtgtaacagt ttttccactt ccaaaaggtc ccggtatagc tgctacccca ccttttgtga
    47161 ttgggaaaaa tgtatctata actctttgac ctgtaacaag aagtgaatct ggtgttttct
    47221 tttctctata tggtctttct tttctaactg gccatttttg catcatagtt acaggtatat
    47281 cattaccttt ttcatctgta ataacacaaa ctgtatcttc aacagtaaat tcaccagaat
    47341 atatttcttt tatagtaccc ttaactccat aaggaaccat aattttacat tctactactg
    47401 ctgtttcttg aacgccacca attatatctc ctgctacaac cttatcacca acagattttt
    47461 taggtttaaa ttcccactta gttgttctat caagtgaaga tacttcaaca cctttagtta
    47521 tattagtacc tgaaatatca tacatttttt caagtggacg ttgaatacca tcataaatag
    47581 ttgaaataag acctggtcca agttcaacac tcataggcat tccaagagaa actacttctt
    47641 cccctggtcc tagaccggaa gtttcctcat aaacctgtat agaagcttta tctccatgca
    47701 tttctataat ctcgcctata agatgtttat ctgaaactct tacgacgtca aacatattcg
    47761 catctctcat gccctccgct acaacaagcg gtcctgacac ctttacaatt gtgcctgttt
    47821 tcatattctc tcacctgcaa tcatatactt tatcaaaatt taaatattcc ctcctatatt
    47881 ttattagcac atatctttag caccaataaa atttgagatt gtcttttatt caactttcat
    47941 ctattcttta aataatatat ctgcacctat agcacgttta ctagattctc ttacttcatt
    48001 catacctaat cccatacttc ctccaatacc tggaataact ataatggcag gaagttggta
    48061 atctttgtat tttgctattg attcttttgc aagcaatgat gcctgttctg taatataaat
    48121 tatcgcatat tcatcttcaa ccaacttatg tatacttttt ttaatttcgt cactatcata
    48181 ggctgggaag atatctattc caagagctaa aaatcccatg atgctatctt tatctccaac
    48241 tactcctacc ttatacataa gcatccctta gcctttcttt tatagtatct gcgtctatat
    48301 tattaagctt actagtcaaa ataattctaa ttgtttttat ctctgattct ttagcatata
    48361 aatatgcaat tagtggttct aatgtaagag gcttaaattt tgctcctcta acatattcca
    48421 taagatagtc atcacaaagt ttttcaaaga cagtaaaacc actatccatg ccatttttac
    48481 agacatcaga gtaagaagta gatttaaaac atgatgctgg agtatctgag gaaaaagctt
    48541 ctaagaattt ttccttatca agtgagccac ctgaaacaaa cacattcata aacatatcaa
    48601 aatctttctt catatttttg actcttataa aactctttag atttgttaaa tcgcaaactt
    48661 tcataacata atctattaca aatttgtttt ggctttcttt agcagtttct ttcatactgg
    48721 taaaggttgc tttatctaga acaatatcaa ccatctggcc attttgtgtc ttattataaa
    48781 catcaaatgc ctctaatata gctgatgaca ttatcttagg taaatcacta aaactacgat
    48841 ttgctaaaga ttctctaagc ttaataagag gaattgtacc tccatcaatt aaatatttct
    48901 caccatctac accagaaatt tcttctttaa tcagcacttt taaattgtga tagtcatttt
    48961 tataaagaaa tacatcaaca aatttttctt caggagctag acttttaaga atttcatatg
    49021 tctttgataa ttcttgagtc aaaatattct caaaattatg tatattatta tttgatgtat
    49081 cagcataacc tgcctcagct ataattctta aagagtcttc agggcttttg gcttctgcca
    49141 tttgaataaa catttgtctg ttaagaagct gtttttcaag cactcttatt cgtgcaacgg
    49201 catagggata agttttttct tctgccattt tttaacccct ccttaattaa acaaaatttc
    49261 tgctgcaatt tgttcaatat attcatgctc tactgcaatt atggcttcaa atgaataatt
    49321 gtactcaatg tcaccttttt taactatgaa acctccagta atatctctag tttcatcaga
    49381 cactttaatg cctttttttg atagcacatc ttttaatgtt tttttatctt ttttagataa
    49441 tactatctct gaatcatctg tacaatttga tttttcaatc atgtttagta taatctcttc
    49501 atattcatta tctttaagat ttaaaagttt gtttttagct tctaaaatta cctcttctaa
    49561 aatctcttgt ttttttgata atatttgctt tttagcctcc atataagctc ctgatatttc
    49621 cttagaagcc gctttttctg cttctgcctc tgcaagcttt gtataggaag ccatttcctt
    49681 ttcagctttt tcattagctg aatttacttt taaatctgct tcacttttag ctttatccaa
    49741 tatttcttgt gcttcttgtt tggcatcagc tataattctg tcaatcattt tttgttcatt
    49801 acccatagga gtgcttcctc cttttcattt tttttactta tgaattataa tttataatta
    49861 accaattgca attccattgt aagctaagaa agaaacaagt agtcctaaaa gagcatatgt
    49921 ttcaactatt gctgcaaatg ttatagcttt accaagttct tcaggtcttt ttgcaactat
    49981 tccaactcct gctgctgctg ctttaccttg tgctatacct gatactaaac caactaaacc
    50041 aattggaagt cctgccataa gaagttgtaa accttgagct gatgttggta ttgcatctcc
    50101 accaataatc cctgcttttg ataaaataag gaaagctatg ataaatccat acagaccttg
    50161 tgtaccagga agaagttgta aaactagaag ttgaccgaat ttactagggt cttctgttac
    50221 aacacctgct gcggcttgac ctgctattga agtaccttta gcagaaccca tacctgcaaa
    50281 taatgctgct atagcagctc ctgatattgc caaaaattga ccatttccta acgcgtttaa
    50341 aaattccata tttacatctc ctttttatta atgcttgtat attttgtttt atacttaaac
    50401 ggaacaaaag gaactcctcc gccttcataa aacttattga aaaattctac atactgtaac
    50461 ctacttgtat gaacatatgc acctagtgca tttataagaa ggtttattgt gtgtcctaca
    50521 acaccgatta caactgcaag tatcggacca gctatagccc ctaaaaggtt gataacttgt
    50581 gctataactc ctgttgttaa acaaagcgcc ataattcttg tataagataa tatatcagca
    50641 aaataacttg ttatgccata gagacttgaa aatcctttaa aaagcttaac aggaatacct
    50701 ttaaagcttc ttccttgagt aagtacaaga cctatagcac ctataattgc taaatatttt
    50761 cctacttctg aaaatacacc tatgtcccct gcaacaaaag gtattataag tagacataca
    50821 cctgttatac acaaatacca aaaacctatg tcaaagattg catctattac ctttccactt
    50881 cttattaatg tatatgcttt tattcccaaa cctacatata tatggataat accgaacagt
    50941 aacgacattc ccataagtgt cataacatcc tctaaaggat taattaatgc ttttatagga
    51001 attaaatccc ccaaaatacc cccgaatata agaccccata cagtagttga aactccacag
    51061 ataccaataa gtttaattaa atttccttca cctttttgta tctttaattt atatacaaca
    51121 aatccacaga ctactgcaat tattaaccca tatgcggcgt cacttagcat cattccaaag
    51181 aatacaacaa agaagaaagt taaaattgta tttgggtcaa tatcttttgt acttggatag
    51241 ctgtacatgt tagttacact ttcaaaaggt gttactaatt tattgttttc aagcagaatt
    51301 gggaagcctt cttctttact tccttcttca gtctgaacac aacaatcaaa cttttctgta
    51361 atttctttaa ttaattcatc tgcccttttg cttggtaacc aaccattaag acagaatgtt
    51421 gtttttgttt tgacaagtct ctctataatc tttttttggt ctctttctat agtgtaaaaa
    51481 tcatataggt tttcaagcat gtgtatacta tctgcatgtc ttttaatttc ctctgttttg
    51541 tccttagaaa cgttttcctg cctaattatc atttcattgt atttcttgat agcttgtaac
    51601 ggtgtacctt cttcatttgg taatgaaacc actgaaaaat taaattcttt taaaacttct
    51661 aaaacatcat caaaagtttc ttttaaagta attaaataaa catagttcat taacttgtca
    51721 gaattgacaa tcccaataac actttctggt atttcatctt ctaattttac tcttaaagca
    51781 tcaatatttg cgcttatggg cacagttcca agtatagttt ttgtatactt agtttccata
    51841 ttttcaagag gaatatcaaa gttcacccat ggttctaaca tacttttatt gttgttaagt
    51901 aagtttatgt tagaccttat tgtacctatc tccttgtgca aattgtttaa ctccagagct
    51961 tcagcataaa cttgcccttc tttttcacta tcaattttgt taaactctct ttttggtgca
    52021 agtagggatt ttttttgttt cactagtgaa gaaacaatat taattgcttg gtttacatga
    52081 gacattttct gctctagcat tataacttca gattcttcac tatcttttac aagataattt
    52141 tttaattcct cctcctcagt aagaaaggca ctatcatcaa tctgaacaaa cccacggttc
    52201 ataagtagct ttaaaatctg gcttttttga gcttctaagc caacaataga tattttgttc
    52261 atattaacta ttgccatatt atctcactac ctttccaata accgcttcaa tagccttact
    52321 ttttctttct tcagcagttt tttcaatttt cagacattca cttttagcat tagctaagat
    52381 tttatcaacc tctgctctac tgtcggtgac tgccttctca ataagagttt cttccataag
    52441 ctttgcttgt tttcttacat cagcaaatat gctttcacat ttgtttacag catcagctct
    52501 gatagactct gcatcagtaa cagcttttac tttgatactg tctgctgtct gctctgcctc
    52561 aacaatgctt tttattattt caaaagccat ccaaatcacc tctcttacct ttttactatg
    52621 tatataacca attagatttt tttattctaa ttagattact acctcattgt taaagtataa
    52681 acagcatttt tatataataa ttaatatttt tcaacgttta tttcttaata aaacatattt
    52741 tagaatagta cttgcctaga tttacatcct tttttgactt acttttagac tatgacatga
    52801 gtatggtttt taaataccat tattttcaat aattataatt taaagcgata tagtttgtca
    52861 atagcgttat tttgttttaa aattaacgct atgtcgacat tttaaatttt tattttattt
    52921 tttttataat tttaatttaa tttattacat ataaaagttt tttatattct ttttcaacct
    52981 ctaacccttg aagtttattg ttttttttga atttttagaa atatctcgca ctattttatc
    53041 taacttttat aaatcatcaa gctatatcga tttttgtcac cttattttca atctaaattt
    53101 tttatatata gttagaaata aatcaatatt tttaaagatt gataaaactt acacttagtt
    53161 ctaatagttt tattttaagg caatgtatta atcttaagta gataatatca ttagttactc
    53221 tatatattcg acaacattat aaaaaaatcc tcgtaccaat cactccaatc ttcaatagga
    53281 tatgctgctc ctccatttac acttttcagt aggtttgatg tatattcttc tcccatggaa
    53341 aaaagttccc ctactgttat atcataacct ctagatgtaa caaccttaca aaatgaatcc
    53401 ataacatcac gttgttctct tgtttttata agttcctttc taaattcatc atcattctta
    53461 gcttcttcta aaattttagt taatttttca ttcattatgt atttctcctt ttaagtagtc
    53521 tttctgtaaa cttcaataag ctttatataa aaaggaagcc atcctaaata aactaagatg
    53581 acttcgctta actgttagca acacatttac tagtataaaa taattggtgt acctggttct
    53641 atattttcat atattttctt tgccttagaa aaaggtgtat ttacacatcc atgtgaacct
    53701 gaagacttat atatacctcc accaaaagaa tttctccaag tagcatcatg aatgcctatg
    53761 ttcccattaa atggaagcca ataacttaca ggtgaactgt aaccttgacc agttaatgaa
    53821 acatttctag ccttataatt aagaggatat atacctgatg gtgtagcaaa tcctctagct
    53881 aaatcgcctg ttacaacatc tgttgataca agcatttctc catttttaaa gaaccacata
    53941 tgttggccag aaagactaat ctctacatat gtatttccaa tatcatcttt attacgagat
    54001 acagcttttt gtatgtaaat aggttctctt gtaacatttt taccagcttt tacaacatca
    54061 actaatgctt cagcttcttt ctttttatct atcaaccaac cataaattcc acctgaaatt
    54121 gtaatatctt tacctgtggc agttttaaat ggtcttgagt caccaaaagt actgtatttt
    54181 ttacttaaac ttcttgtata ttctaccata gcatcattat caaaagttat gtcataattc
    54241 ttttctgagt ctacttttaa ccatttatta atttcagaac catctaaagt ttccttacgg
    54301 tcactaaaat catatgtaac gactacacca acatacttat ctaatgtctt aatagcttgt
    54361 ttcaactttt ctgaatcctt tgtgttgttt ggtgcttcat aaactttact ttcatccaga
    54421 gaaatagaag tattcccaga attaattgag ttttgtacta acttataaaa atcatctttt
    54481 ttcactttgt ttccaagtac ctctggtact ataacataag cgtttttagt gctatcatac
    54541 ttaggataag cactcttagg ttctacaaca tttttactat caaaacaaga taaagcatta
    54601 aacctttttt ctagtaaatc tttattatat gaaagagtta tcatattatt atactcttta
    54661 gatttaaata ttccaccaat ccatccaata gcgctttgac tatcttttat cttttgaact
    54721 ttatcatctg gtgtaaattt taaatcaata tcttttgctt ttatttgttc tttcttattg
    54781 tttctttcct ctaagtttaa agtataggaa ttagtactag atgttaacaa atcatttact
    54841 tgctcaactg tttttcctga agcctttacg ccattaatag tcgttccaaa gtaaaaatga
    54901 tttctaaaaa agattgccat tcctatataa gctacaaaca aaacacctac aactatagct
    54961 atcttaaatt ttagattttt ttgtccactt tcttgtttta cctcttttcc atcaatcata
    55021 attaaatcct tccttacatt gtaattacct atgtacttta ttggttttta aacaaaacca
    55081 tgtatgcctt attttatata cccaaattac tgtatcattg tttatatttt taaatatata
    55141 gttatttcca taaaaccatt ttatctttta gacaaaatga ctattataaa tcaccccttt
    55201 caaaatccct taaatacaat ctctgctata ttaatatatt accatacttt taataatcgt
    55261 gggttatcta tctgtttttg taacactaaa ctactctttt aaaaaagaaa ctaattttat
    55321 gttagtttct ttatcttaac ttatttttat attgaatttt agcttaacat aacgttgtta
    55381 tatagattct atttaattat tttagtttct atatcttttc catcagcata ttctttaagt
    55441 aactccttag catatccaaa atattcttca atatcagatt cttgactaaa agaaaatata
    55501 caaattaata tttcatttgc atcttctgtt atcattgctc tttcagaatc actagttgga
    55561 ctcccaaatt tacccactga atcagacaat ataggaagat tttctatatt tattaattct
    55621 ttcccaatac ccttatactg ttctccttca tctcctacag taaaacatac tggtgaatgt
    55681 aatttgctta cattatatgt acctactgga tataatgatt ttattgatat taaattgttt
    55741 atttctacta tattatttat tgtataaaga cccttccctt gaataattct tctaaccaat
    55801 gcttctgaag atgttctata tctacttgga ttttttccaa gctctttata agcatttctt
    55861 gcatctttaa ttttatctag tttcgtaatg tcttcaactt ttagactttg atttatagta
    55921 tcacaaaact catttatttc attaattaat ttttctgagc ttttatttac tgctactttt
    55981 gcttgaatgc aacccaactt agcattaggc catctttcct taagtttgtc atcaatactc
    56041 acatctatca tacttaaaca actcctatac actaaatttt tattaaagtt tttatcatct
    56101 atacattata gtactattct tttgttaata acatgtttat tagcaaaata aatttatttt
    56161 taatataatt tttataaaat acaattattt tttgctttat attaaacaat acattgataa
    56221 aataaaaaaa cactaattat ttagcagtga tttattaaaa tcaaacattc ataatagtgc
    56281 tttatataaa aattaaattt tgttttaaag taaatctaaa aaattcttag ctggcattgg
    56341 tcgtgcaaaa taaaatcctt gaactaaatc acatccaaat tgtttcatta actcagcttg
    56401 ttctttactc tcaacacctt ctataattac aactaaatct agtgattttg ctagagacac
    56461 tattgatttt gctatattgc gacttctctc atcatgttca ataccattaa gaaaagactt
    56521 atctagtttc aatatatcaa ttggcatatc tcttaaacaa ctaatagaag aatatcctgt
    56581 accaaagtca tccatagaaa tagaaaatcc atacttcctc aactcattta aaacattctt
    56641 taaaatattt aactcattaa tagccacagt ttctgttatc tctatttcaa ttaaatctga
    56701 acttagatta tacatttgta ttgttttatt gataaatttt acaatatctg gttgatacaa
    56761 ctcaactcta gataaattta ttgaaactgg aactatcttt ttaccttgct ttttccattc
    56821 agataaatca ttgcaaaccc tttcaaatac aaatctccca atttttaaaa tcaacttact
    56881 tttctctgct attggaataa atactgcggg acttataaaa ccatgttctg gatgattcca
    56941 tcttattagt gcttcactcc ctatcatttc tccagttaca agtgaaaact taggttgata
    57001 atacacttca aattgatttt ttaccaatgc tatttttata tcatctaaaa tcattgattc
    57061 ttcactaagc ttattcctaa catcttcatt ataaatagca taatttatat ttttaccttt
    57121 agcaacagac ctagccatat ttgctttatt tacagccttt tggatatcta cttcaccttc
    57181 ttcaacaaaa taaataccca cacacagaga aatttcaaat actatactca aattactttc
    57241 aatattattt cgtatactat caatttttct tatacgttta ttttttccat ttttataatc
    57301 acagaaaatt gcaaaataat cacttgaaag tcgtgcatat acttcacctt ctgttaattc
    57361 ttcttttaaa gcttttgcta tcgtaattaa taaattgtca ccaaattcat atccaaaagt
    57421 atcattaatc atcttaaagt tattaatatc aaaatatact ataatataat ttaaagatgg
    57481 tttatctaaa agatacttac tacactctct ataaaattta ttttgatttc ctatatgggt
    57541 aactttatct tcataagcca aacttaatat ttctttttca ttggattttt ttatataaat
    57601 tatataaaaa ataattgcaa taaatattaa tataataaat aacactgcat atatagctct
    57661 gttgattatt tgttctgaat tactaaatac aacagtttct ggaattgaaa aaatcaaatt
    57721 ccaatctttt atataattat tttcactatt cccattatcc atagtagcat atcctaaata
    57781 tcttctttct tccaacatat ttaatacagt tgcacctgtt ttattttctt gcaattcttt
    57841 ttttaatcta tttatttctc gaatatcatt atcctgtttt aacaatttat aaatattttt
    57901 tgataggcta tcctcttgcg gatgaaggat aattgtacca tcatttctta caacataaga
    57961 tatactatta ttttcataaa aagatacatt aataagtttc atcagagtat tgatattgta
    58021 agtacaatat aaaactccaa ctactttatc attatttaat aatggtactg aaatagtaat
    58081 cttctttata gacttattta cttcatcaac atatggactt gaaacatact tcttaccttt
    58141 aattgagttt ttgaaaaatt ctctatcttt cactgaaaac ttatcacctt tggtattttc
    58201 agcattacca tttatatcaa ctattgccat agtgtaaaat ttttgatttt ccatttcatc
    58261 taaaagattt ttcatttttt cctcattagg tatatcttga tttaaagcat aatagttaga
    58321 aaaaatattt aaaacatcta aatctttttc aatttgtctt tgtgctagtt gagagccttg
    58381 tactgcaact tcttttatat gtatttttgc ctctgacata agaagatgtt ttgtatcttc
    58441 tatatagata taccccattg tcaaaaataa tgatgttaaa aaaagaattg taattaatgt
    58501 aaaactttta tttctccctg tcattcgact cctcccctaa ttagtcaata cttcatatag
    58561 ataatatatc ctttttatac cgattcatca tttatattgt atataactat tcttaaatgt
    58621 tacacataaa atcaacctaa taaccataaa aacattcttt tattatcata ataacactat
    58681 ttagttgaat tttaaattaa agaataaaaa ctatcaattt ttctaaacaa aaaaactctt
    58741 atacaataaa actttgtttt atgtataaga gtttttattt tatctatttt atagtaaatt
    58801 tatcttccat aatatgaatt taaatacata tcttttattt cattcataag tggatatctt
    58861 ggatttgctc ctgtacattg gtcatcaaat gcttgaatca ccatttcatc tattgaatcc
    58921 ataaacttag gttcttcaac tccaaactct tttatagatt taggaatacc aactttaagt
    58981 tttaaatctt caatagctac caataaactt tggaatttct cttcatcaga atttcccttt
    59041 aatcctaaaa atgaagctat ttcagcatat ctttttaatg cttctggata tttatattga
    59101 gagaatgctc ccattttctt tggtgcatct gaagaattaa atttcataac ttctgttatc
    59161 aaaagagcat ttgcaacacc atgtggtaca tggtggaaag cacctaattt atgagccata
    59221 gagtgacaaa cacccaaaaa tgcatttgca aaagccatac ctgccattgt agaagcattt
    59281 gccatttttt ctctagcttc tgggtctttt gcaccattat catatgcacg tggaagatat
    59341 tcaaatatac tcttaatggc ttgtaaagct aatccatttg tataatcagt tgcaagcata
    59401 gatacatatg cttctaatgc atgagttaat gcatctatac cagaagcaga tgttaagctt
    59461 tttggcatat tcatcatcat atcagaatca actatagcca tatttggcat cagttcataa
    59521 tctgctaatg gatattttac ccctgtatct tggtcagtta taacagcaaa tggtgttact
    59581 tctgaaccag tacctgctga tgttggtata gctacaaaat tagctttttc acccatttta
    59641 gggaatgtat ataccctttt tcttatatcc ataaatctca tagctaggtc ttggaaatct
    59701 acatctggat gctcatacaa tgtccacatt atctttcctg catccattgc acttcctcca
    59761 cctatagata ttattacatc tggattaaat tctctcatag ctttagcgcc tatcttagca
    59821 cattccaaag ttgggtctgg ttctacttca aagaatgtag tgtgcttaat attcatttca
    59881 tctaataaat cagtaactgc tttagtatat ccattattgt aaaggaatgt atctgttaca
    59941 ataaaagctc tcttcttatt cattacatct ttaagttctt ttaatgctac ccctaaacaa
    60001 ccttttttga aataaacttt tcctggtgat ctaaaccaaa gcatattttc tctcctctca
    60061 gctactgttt taatattaag taaatgctta actcctacat tttcagatac tgaatttcca
    60121 ccccaagacc cacatcctaa agtaagagaa ggagctagtt taaaattata taagtcccct
    60181 ataccacctt gagaagaagg agtattaatt aaaattctac aagttttcat ggctgaagta
    60241 aacttatcaa gtttttctct ttgatttaca tcatcaatat ataatgaaga tgtatgccca
    60301 aatccaccat cttctactaa tttttctgct tttctaactg cattatcaaa atcactggcc
    60361 ttgtacattg caagtactgg tgaaagtttc tcatgagcaa aagcttcttc tatttcaact
    60421 gattctactt cacctattaa aaccttagta tctactggga cttcaaagcc tgcaagtttt
    60481 gctatagtac aagctgtttg cccaactatt ttagaattta atcctccatt tactaaaatt
    60541 atatttctta ttttttctgt ttcatcttta tctaataaat aagcaccacg ctctttaaat
    60601 tcttttttga cttcattgta tatattttct aatactataa ccgattgttc agaagcacat
    60661 atcataccat tgtcaaaagt cttagataca agaattgagt ttacagcagt ttttatatct
    60721 gcactctcat caattattgc tggagtatta cctgcaccaa ctccaatagc tggttttcca
    60781 gatgaatatg ctgatttaac cattccagga cctcctgtag caagggtcaa atccacttct
    60841 ttcataacag ttgtagttaa ttctaaagat ggctcatcta tccatcctat tatgccttca
    60901 ggagctccag ctaaaacagc agcctcaaga acaacttttg cagcctctat agtagaattt
    60961 tttgctcttg gatgcggaga gaaaattatt ccatttctag tttttaaagc taaaagtgct
    61021 ttaaagatag cagtagaagt tggattagtt gtaggtacaa ctgctgcaat aacacctatt
    61081 ggctcagcaa ttttagtcat accaaatgct tcgtctttct ctataacacc acaagtttta
    61141 gtttctttat atgcattata tatatactct gaggcataat gattctttat aaccttatct
    61201 tcagcaattc ccataccagt ttccttttgt gccattatag ctagaggaac tctttgctta
    61261 ttagctgcca aagatgctgc taaaaaaatt ttatctacct gctcttgagt atatgtagca
    61321 aatatttttt gagcttctct gatttttgct agttttttaa ctaagctttc aacgttattt
    61381 acagtatcta aaccaactga cataacttca ttttcttttt tattttctat tattacttgt
    61441 tctttatgag ccattttaac aacctcctga gatagttttt tatttattat gttaatttat
    61501 taacattata gcatttactt tttctatttt caagttattt ttttaacatt ttatttttta
    61561 tctaatataa catcatttct actaataata tctagcttta caaatactta atttaactag
    61621 atatcacttt attttttacg caaatcacta ataaaatcat ctaaaaactt aaggataacg
    61681 ttattttttt aacgtttata tttttgtatt ttttgcaaat aaaactttat aaaaagaatt
    61741 tttaatatga tattctatca agtttaaaaa taaaaatact tgaaaatgaa aataataact
    61801 tgattaacaa gttaaagtaa atatagttaa atgtacaaac atttacaaca tatagtataa
    61861 ttaaaaaaat atatattcat gatttttata tactatgaaa taaaatatat ttaatattat
    61921 atagataaat ttgaagttta taagaaggag gataagcatg aaaaaattaa aaacaattag
    61981 cattttttct ttgattataa gtgtaatttt aacgattgga ggtattggaa tagtaactta
    62041 ttatgtagat aatttgttta ttagaggact atcagtattt gttttaatta tgtcctcttc
    62101 ttttgtatca acaacagtta gattgatatt tgaagagtca aaacgatata aattctaatt
    62161 taaaaattgg attaagtaaa taattttaat ataccaaaaa ttacggagat tagctcaaaa
    62221 tagacttttt agttcatgat gcattttttt gtatgaaatt aaatatattt tcatcatttc
    62281 aacaatgaaa aagaggcttc tctctatttt gagacagcct ctttgctagt attgtatcta
    62341 atacataata ttataagaac caattctatt gatgtggact ttttataaca ggctgtagct
    62401 gtttgttctc tagagcaagt tctatagttg gaattattag ctcagtatgt gcaatcatag
    62461 aattaggctt tgctttgcaa ttatcttgac caaaaggtac aaaatatata tttttagaat
    62521 taaggagaat accaatattt ttgaaattaa agcttagagc atcattcgtt gatattgata
    62581 ttactagtgg tttatcatta cgaagatgac cttttgctgc cattaatact ggtgaatctg
    62641 taataccatt tgctaatttt gcagcagtat ttccagtaca aggagctatt attataatat
    62701 cagcaatccc tttaggtcca aatggttctg cctcttcaat cgtaactata ggtttatttc
    62761 ctgtaagttc atatgctttt ttcataaact cttcagaatt tccaaatcta caatcgatat
    62821 tttgtgatac atctgaaaat atagtgtgta cattagctcc ttctttaacc aaattctcta
    62881 actctataaa tattttatca taagtacaaa acgaaccagt aaatcctact ccaattgtaa
    62941 gtccttcaag tctcatttct aatcccttct ttcatttata atattttcta tagcgttaac
    63001 aagaatctca ccagatgtct ttggtgaata tttaccagga agcccaaggc aaagagtcgc
    63061 attaatccct acttctttaa caacagaata atctactcct ccaggagaag aagctatatc
    63121 tataatagtt acttcttgtg atactttttc taatctttct ttcgttaaaa tacactctgg
    63181 tatggtgtta aaaatataat caaattgtaa aagtttttca tcaagttgac ttaaagaaat
    63241 actggaatat ccaaatgcat tagccgtact tcttgcatca ttatttcttg caccaacgca
    63301 tacatttgca tccaaagcgt ataatttttt tgcaagtatt tgtgcacatc ttccataacc
    63361 taatactaaa cagttacttt gatgtatatt gattatactt cttttaatag cttctgctat
    63421 agccccttca gctgtagcaa ctgcatttaa aatagcaaca tcattcattt tcataaaatc
    63481 ataaatctga atagagtgtt gctcgcaaaa tttacaaact ttagttggta tgttacctcc
    63541 aaataatatt tgattaggag ataaattaat tagcaagtta tcaatagtag catcaagaca
    63601 atcatccgta ttttcaatat taacttgatt ctttgtaaat ggaattggac ataaaataat
    63661 ggaacttttg gatagtgctt cagctagtga agttgatttt ccaacgattc catttataat
    63721 ttcatttgat atcccataag tgactacaga atacccttta ttaattagtt tttttaccat
    63781 gtacacttgt cgcaggtctc ccccaataac agttatatcg tattgatttg acatattgta
    63841 agcctccttt actatataca atatgttttt atcaatacat tggttcacta aaattactaa
    63901 atattatact tataacattt ttaataatgc taatctatct atcttttcag aagaggtata
    63961 tggaaatgaa tttaaaaaga taattttctt tgggatttca tttttcatta gattatcttt
    64021 cattttaatc aacaaatctt tcttcgtaat tttgtctata gttattacaa ataatacaat
    64081 ttgatttcct cttatagtgt ctgagtacgg caatacaatt gcatcttcaa tttgaggaat
    64141 ttttttaact tcattttcaa tcttagcaga actaacttta aatcctccaa tatttacaat
    64201 gtcatcactt cttccttcaa atattaagta tccatcatta tcataatatc caatatcatt
    64261 gacagaataa ggtttggttg cattatatac tgcatattct gtattcacaa aaatttttcc
    64321 tcctctaata tatatatcta ttcctggaaa tggtctgcct acacttagag gtttttcaat
    64381 tagttcatca tagcataaat atgtaatata atttaattca cttgccccat agtataaaat
    64441 aatttcagac tttggcatat atttttttag atttcttgca gtatattcaa ataataattg
    64501 agaccctgta aaaatactta ctactttgag tactggttct tttaagtgtt taaccaaaag
    64561 ttgaagtttt gtaggtataa ggtatatgtt agttacatta tattgtttta ttgtccttat
    64621 ccatgattta caattaagac ctgaacttat tacaatacta cctccttcat ataatactga
    64681 cattatagaa tttagattcc ctgtaaaact aagtgttcca ttaacaaaca agattgattc
    64741 cccagagata ttaaaaatac cattttgtat aggaaaaaat ccagcccaac tctcatatgt
    64801 tctatataat acctttggaa cacttgtaga acctgaagat aaagctccca tacaaatact
    64861 tttatctaaa taatttataa ggttcttata ttgatacata tatagattaa atttaggatt
    64921 aaaaatagaa aaattaactt tttttgctac ttcattattg ttatcatcat ataaatcaat
    64981 aacgtccata gattcatcag atataatgat tgaaatatta ttttttaata aaatatcatc
    65041 taatactttt ttagaaagat tataatgaca aataatagga atattttttg aataattaat
    65101 agcaaaaaaa ctaatcaact gaaacattat atttttagaa tatattaaaa ttgagatatt
    65161 ttcacctaaa gaaatttgtt ttcctagttg ctcactataa cttaatatat tttcataagt
    65221 atatttttct ccatctataa ttaaaaaatt tttattttta tgcctctctt tttttattct
    65281 taaaagttca taatagttca taatctacac cctttcaagc agtaaagctg aacctagacc
    65341 accagctgca gctatagagc aaattccata tctaccctta gttttttcta atgcttttaa
    65401 aagatgtaat gtaataattg cacctgaagc accatatgga tgtccatacg ctaaagctcc
    65461 accaaatata ttatatctct ctattaactc tggatacttt ctttgaaaca acacatctat
    65521 aactgcaaat gcttcattaa attcaacagc agatacatct aaataattaa ggctttctat
    65581 ctctaaaagt ttatccattg cttgtatcgc tgatgttgga cttaaggttg catctgttcc
    65641 tattgtgcat gtattaataa tttttgtttt aggcttcttt tttacatgtt ctaaatactt
    65701 cttagaacat attataataa atgaagctcc atcattaata agacatgaat ttgctgcatt
    65761 tgtaatagtt tcttttccta aaattggtgg catcctatct aataaccttt gactcatctt
    65821 atcacgtata ccttcatcat aggtactatt atttatagga gatataatat cctcaagtat
    65881 tttttcttct cttgcttctt ttgctctttt atgactttcc tttacccaaa aatccaagtc
    65941 tgctctctca atttgatata actctgctac tctttccgcc ccttcgagca tactattttg
    66001 actattatca tcaggagaaa attgagcaac tgtataattt ggattatttg tattatatcg
    66061 cttatcattc ttatgatatg tgcgcattgg ttgtagagaa ctactttcaa aaccacctgc
    66121 aataattaaa ttacactgac cagatttaac ctttgaaaaa gctatgtcta tactcatcat
    66181 agcagaagca cactgcatat ccacagtaaa agcaggtact tcatttgaca ctcctgctgt
    66241 taaagtcatt aatctggcta tattccctcc tgtcccaact gcatttccac atataatctc
    66301 atcaattttg tcaatttcat actttttaat taaatctttt aaaacacttg ctcctagtaa
    66361 ttcaggttga acaaattgaa aaattccatt ttttagacct atatgacttc tcaatcctcc
    66421 taatataaaa acttccttca tacattacac ccttccttat attatagatt tatcatagat
    66481 ttcttctgag tacattgttc agtttagtgc ctaaaaaaga tgcaataacc gactttattg
    66541 tatctccaat taaaaatggt actactgctg caaaaagtgc tgcttctact gtcatttgat
    66601 taaaatagca cataaatact gttcccataa aatatataat aggcattcca acaaaaattg
    66661 ttattagtat atatctctta atatcattat ttcgtccttt taataaacta ataattagtg
    66721 ctgcaaacaa aaacccaaaa taaaaaccac ctgttgggct taacaacttt ccaataccag
    66781 atgtacctcc accaaatact ggaatcccaa ttaaacctat cattatatat acaccaactg
    66841 tactaaatgc ttgtcttggt gttaaaatca aagctatcaa atttatcatt attgtttgtg
    66901 ctgttactcc tgctggagta aatggcaatg gtatatatat gtaagatgat atacacaata
    66961 atgctacaca tattgacatt ttagttaaat cttgaatatt tactttagta tttttcattt
    67021 ttataattcc cttcatatgt cttatttcat ttagtttata tgtacatata caaagcctct
    67081 attaaagctt tgttttagta taaaataaag acgttttttt gtcaaccatt acaagtattt
    67141 aggtttacgt ttaatttata agtaaacttt atactgttac tttatatttt aatagtcatt
    67201 tataaaactt tgcttaaaaa cagctctatt tagactatac ttgtaaatta taatatgata
    67261 tatttttata taagatttta ttttatataa aaaatagtaa caaatagttt atacacaaga
    67321 tatttaaaag gaagtgatga tatgaaagtt aatgaaaata atataaataa tatagatgaa
    67381 gagttagatg aacttagtag gaaagtttac ttagagtcac cagatttttg gataagtatt
    67441 tctgacaaaa taatagataa agtatttgat gaaatgttat tattttttgc agttaaatat
    67501 aactatttca atatagtatg ctttgctatt gaaaacaatt atctggattt aaacatgcct
    67561 tcaaaaaaca aaaaatacac taatataatg gagcatttgt tagatacatc aaaacagaat
    67621 gaagataaaa aaatttataa ttatcttcta agcttaaata aaggtaataa cctagatgaa
    67681 agaccaattt tagatgaatt aaactgtaat attagtagtg atacttttga aaaaaaagat
    67741 atatatcttc caaagtactt atgtccaagt tgtaattcta atatatttga tacaggttat
    67801 aaagtatcaa atgacataac atataaattt tcttctaaag aagctgaacc tattgaaatg
    67861 tctagtgaag tttcttctat cagatgttgt aattgtgatg aattattaga agatattaca
    67921 cttaataaac tatataacct atgtaaaatt caaaactgta ataattgtgg ttctaattta
    67981 actaaggtag gaattattgc aaagaaaaaa atgaactttg atgagaatac tgaaaaattt
    68041 aaagatgtct caacatctta ctgttgtgga aattgtgact ctgaaataaa cttatctcaa
    68101 agagaacatt ttaagattta aatatttata ggctaaagta ataagattac attattagat
    68161 actgtataag ttttttaaat ctgtaaaaat aaatacactt ataagaatta aaattgctat
    68221 gaaactagtt cttgctgaac taatttcatg gcaattttct ataatctaat aattattaaa
    68281 ttttaacaaa cctttattag cattccataa ttattcaaaa ttctttcaaa taccctatag
    68341 tttataggtg aaagtatctt taatattata tcaaacttat tatctttaaa tagtaaattt
    68401 gttaaatctg aaatacacca tacaatttta ttttcctctt ttgtagctat atcaattctg
    68461 attaacttac atctataata aatatcttta tcttcatata gtctatttta aaaattactc
    68521 ttataaaaat caaatagaaa ataaattaat tattctttta atagaatgta atcatattat
    68581 ttactatatt ccattatgta ctctttaaat cctgaatctt catttacttg ttcttttact
    68641 atattaaaac cttttttatt ataaaacaca actgcttttt tattatcttt atatactgct
    68701 aaacttagac ttttatattt tttagttgca taatctaaaa gtttacctcc gatacctaga
    68761 ttttggtatt tgggttctat aaataaagct cctataaagc ttttatctat tatgcttata
    68821 aatcctttta tttcatctcc atcatcatat acaaatgtat ctgatatagg tatatattcg
    68881 tttttaacag tattgtagtt attttgccag tattctttac ttataaagtc atgtgctttg
    68941 attgtacttt tttcccatat ttccatgatt ttatttatgt ctttattgtt taatttcttt
    69001 atcataaatt ttgtttcacc tcatttttgt attgatttta taatgtttat aggtaagtgc
    69061 attttattta gatatgattt agttaatata aggtggatgt tttagtatat gctattgttt
    69121 attctacaat tttatatact gctttattaa tataaaataa gtttctaaat actcgttgat
    69181 attgctaata ttctacttat ctaccactta gttgatataa aacctgacac tgtgtcttgt
    69241 gtgagtcttt tctttatcct atttacatac cacttagtta atataaaacg attaagccca
    69301 gtaaaaagac tcgaatctct tatcgcattt acataccact tagttaatat aaaacgcaaa
    69361 aaatgttgat attcccaaaa ttaatattga tgaatttaca taccacttag ttaatataaa
    69421 acattataga agtacatata ttgctgaaaa acctcttgta tttacatacc acttagttaa
    69481 tataaaacag ttagtgatat caatatgcta agcaatattt ttacaattta cataccactt
    69541 agttaatata aaactttata tttcgtcgca cggattcgtg cgaagggttg gatttacata
    69601 ccacttagtt aatataaaac taatttagtt atatccattt gtttaaccca attagtattt
    69661 acataccact tagttaatat aaaacgtttc attttgcact atatcaagct tagtatacaa
    69721 tgatttacat accacttagt taatataaaa cttttatatt taattttaaa gattaaaggt
    69781 ataaacaaat ttacatacca cttagttaat ataaaactac tagaattatt aaatgaaatg
    69841 aacaataata ttgtatttac ataccactta gttaatataa aacgatttaa atgcaacaca
    69901 aattataaca tctaagaata tttacatacc acttagttaa tataaaacaa gattgaaatt
    69961 tcaacacttt caccttgtat atctatttac ataccactta gttaatataa aacaggttaa
    70021 aaagcaatat aaaagattgc atggaattac tatttacata ccacttagtt aatataaaac
    70081 aagtctcaat aacaacagtc gaagcagaaa aatccggatt tacataccac ttagttaata
    70141 taaaacgtaa aatgagaaat aaaagtaaac aaaaaggttt agtatttaca taccacttag
    70201 ttaatataaa acctgaaacc ctcggattta gtaaccgatt ctatgtcgag tctatttaca
    70261 taccacttag ttaatataaa acaatctaaa atatgttcgt taaaagggag tgtgtaatta
    70321 atttacatac cacttagtta atataaaacg tttcttatac aactcttaac tccaagccca
    70381 ttttggaatt tacataccac ttagttaata taaaacgtat aaaaaaagag ttagaagcat
    70441 tagacaaatg gaatttacat accacttagt taatataaaa ctataattgg gatggtcagg
    70501 tttctgataa ggttaataaa tttacatacc acttagttaa tataaaactg ttttttagtt
    70561 ttcgtgattc aaatggttct ttgttattta cataccactt agttaatata aaacaactca
    70621 tgtaacaagt attaagactt gcacctttaa atttacatac cacttagtta atataaaacc
    70681 ccaaaatagg tataattgaa tacattgaaa atacttcaat caagcaatta taattcaata
    70741 aaaacatcaa aatttgcagt gagcgagcag tagtgcaatt gataacagtt atcatacacc
    70801 ttcaatccct tatattccaa ctgttaacac ctattttagc ataaatatcg ctcactgcaa
    70861 acctatataa acatttcatc agaatactta tcaatcccat aacatttctt tttaattgaa
    70921 ttaggatttt ttatctcata aacatagatt gaatcctcgc tattatcaat aatattttct
    70981 atttcagaaa tacatttatg taactttcca tcagtaatat tgccttcaaa aacagaattt
    71041 tgcgtccaag tcaaatattt tctaagtatc cttctaattc tatttaacga ccttgcttca
    71101 acaatatcat aagtaacaat aacaaacata cttaccacca cacctttaaa actttgtacc
    71161 tttcatcatt tataaaatgt ttaattaatt tataacactc taaccttata aacattctat
    71221 atgaaacttt tctatttaga tttctatgtt ttatagttgt ctgcatctta ttttcaaatt
    71281 cttgcaaaaa tttcttcttt cctttttcac ttaaataaca aattttgtct tgatattcaa
    71341 aatcattctt attaattctt ttattattaa tcaggctaaa aattattgtg tcaactataa
    71401 ttggcttaaa aatttctgca atatctaaac ttaaagaaaa tctctttgtt gatggttcat
    71461 gtaaaaagct tattgtaggg tcaagttgtg tcttatatat ctcacttaaa acattcgtat
    71521 acattatgct atttccaaaa gacataagag tatttactgg gtctttggga ggtctttttt
    71581 ctcttttata aaattcaaaa tcatctttta atatttgatt aaatgattta tagtatgttt
    71641 ttcttattct gccttcttta cccataagtt ctgatatttc tttgacatca ttcaaatcat
    71701 ttagttcatc ctttatagta tcaattaaac tttcatcaac tttgtaatat ctcatatttc
    71761 ttaaaatatg gtatgatgca ctttccacaa aggattttgc taagtataat ctttcatttt
    71821 tatctaaata gcaggctgat tgacatacta aactgaatcc tgatacattt ttcttccttg
    71881 gataatatgt acctgaataa tatccatagt aattgtagaa atgaagcatc accccatatt
    71941 tacttatgta atttataagt tttgtgttta aatctacttc tccatatata tgaatatcat
    72001 caacttgttc tactggtaat atttttttct caccttctaa attgtaatag taaatcgtat
    72061 tttctttcct ttttatattt ccattactta taatataata gtctttatac attgttactc
    72121 ctccttcaca aaacaaaatt cataataagc acattttgta caataatgtt ttttctcaat
    72181 tttgggagga gataactgca tatttatttc atttatattt ataagtgtgt tttcaataat
    72241 tttttctttt tcttctgtca atatcaattc ttcttgtttc ttttccttaa cataatttat
    72301 agttcctttt ttctctatac ccatactatt ctttaaatag tataagtaat agtataactg
    72361 catttcatct gcttctttca tctttgaact tatttttact tcttttacat actcgtcctt
    72421 tattatatct agcttcaaga tatcatcaat caacatttct ttatttttca ttcttttata
    72481 tgaatcttca tgtactatct ttccagacaa tactctacta ctattttgtt ccatagttat
    72541 tcctttagaa aatagccata gttttctttt acatacataa taataattta tttttacgcc
    72601 ttgaactttt aatacttcta aatctattgg cacaaaatca ccttctttag taaattttag
    72661 ccacattgtt aatataaaaa tcaaataaat tggtctattt cattacttat aagcacacct
    72721 ttaccaaata tctgttttgt atgcttattt tcgtgtatat catatttata taaaagtatt
    72781 tttaaatctt caagaccttt cacctgtatg tcaattacat tttgtttagc tttatatatt
    72841 ggtaaattta cagttttttt aattatctct cttcttgctt tttttctctt agactttatt
    72901 ttatttatca attgtctatc atcattacta tatgcattta taagctcttg tcccaactct
    72961 tcataatctt tgattaaagt ttcttcaatt ttattatata tatcctctgg aattactgta
    73021 tatccttcaa tatttctaag tatattttgt gcatctttag aacctaaact atatggcgtt
    73081 atagtatcta aaatatttaa agcactggta aatctctttt caaaagctgt accttctaga
    73141 ctttctttag agtatagtat cttaaccatt tctaccttat atttttcttt catcttcaca
    73201 cattcttttc catttataaa agttttcagt agctcaagtc ctttttctac aatatcctta
    73261 tcataaatac tacctattcc tgtagctttt tctgtatata taaatatatt tgggctattt
    73321 tcttcatact cacgacttct ataacatcta ccaaatcgtt gaaatagact gtcaagtgtt
    73381 gaattttctg tatgaagctc atcaaaatca atatcaaggg atgcttccac taattgtgta
    73441 gtaatccaaa taccattact atcactatct gcaaattctt ttatatattt ttctaatttt
    73501 gctctatctt cttgtatata catagaatgt aaaagattta agttgacatc tattcctttt
    73561 ggtttaacta tttcttctat taactcatat ttttctacag cacttttaac tgtatttact
    73621 attacaagaa cttttttatt cattccactt tgtattattt tacctaagtt ttcatctatt
    73681 gaattttcta caatagacac acaatgtctt attttttctg tgttacatgt caagtcagct
    73741 aagttactat tcattacacc tctttttttt aattcatcta tatatatggt tggcatagta
    73801 gctgtcatta tcataaatct gccacctatc ttatgtatca tttctatacc ttttaccaat
    73861 acagctgcta tttctggtga atatgcttgt atctcatcta ttactacttt tgaatatgct
    73921 aatgttgagt acactttttc atagcctcta tacaaaaaag gaaatttaaa tatttggtct
    73981 attgtggaaa atgtcagttt acaagataat aactttgcta aatctacaat ctcacttgaa
    74041 ttttcctgat tactttcttc taaataatct attgctgttg aatgtaacag acctaagaat
    74101 gtatcatttg cttctcctac tccaattata tttttagccc tatcaaataa tgcatttata
    74161 cttactctta atggtagtgt aaaaaatgct ttatccttat ctatccaaat caaagcagtt
    74221 tctgtttttc ccattcctgt agatgcaatc agtattatat tcttatttct attagattta
    74281 gcaaatgatt gagcctctct caaactacca aactctttca ttaaataatt ttctgtctgt
    74341 tctcctatat ttataacatt attgcattcc acaacttcat gagcagaagc actatggtct
    74401 agtctatgta gtattccttt taacataata tataaattat agtacttgtg atttttatct
    74461 attctttttt ctacactttg tagatatact ttactcaatt tttctgtttt tattggatat
    74521 ctaactttaa attcacgctg tagttcataa actttattta ttaaatcttc atctaatatt
    74581 ttttgtatta aatttttaaa atctttatct ataaagatat ctctttcatg atgatataca
    74641 ataacttgat ttaatactgc tctaagttct ttattttttt tcctgtctat ataactataa
    74701 tcaataaatg caggagaaag ataattatgg cctacattat tttctaaatg agttactatt
    74761 ttaggttcat ttattttagt atccattctg ctttttatta actcttgaaa cggtgaaaat
    74821 gcttttccaa tatcatgaaa ttctataaca aagtcaagta attgccaaaa tatctcttcc
    74881 tctagaaaat ctaagctatt tatatttttt ccataacttt ctcttaatac attcatttgt
    74941 tttaaaagtt catcagtatg ttctctaagt gtttccactg gattagattt agcatataac
    75001 atactctccc tcctaattta tattattaaa atataataaa tctccttcat tatctaataa
    75061 tatatctcca attgatatag tatttccatc ctcaacataa taactatcta ccttattcca
    75121 aacccttttt ttatcttcca cagcattatt atcatagtaa ttattgagtc tgtaacttat
    75181 accatctata tctgtatctt gtaaacttgt ttttggtata tatataggca ttcttatatt
    75241 acattctgat aacttataat tttcataatc atctaattgt atatcctcat ctacttcaaa
    75301 tttattgact tctacaaatc taatatcatt aattcttact aaatcttctt ttctacctaa
    75361 tgataagtgc tcgtcataat ttaataaact atcatatatt tcttccaata aatcaaaatc
    75421 cccacctata tgaattatca aatttacatt caatagcata tgactattca ttggcataga
    75481 tgtaatacta tcttttttat aaaaatatgt tgtctggtaa ttattaaata tactttcata
    75541 tgttccctgt acacttatat taaatggtat atattctgta gcatttaata ttttgtgaat
    75601 taatccattt attgtagaat atggaggcag tggatatgtc tctgtaatct tcatagcaaa
    75661 tggtttttta tagcatgcag tttcttgaaa taaatctaat ttcaaaacct tcatattaat
    75721 tcaccccata atattccttt acttctttaa ctagttgtcc aaaaaattta tctacactta
    75781 aaaaattatc ttcaaacaat cccttaaatt cttctttatt tataaatacc ccatctacca
    75841 ttccaacata ggtaaattct tttaaatctt ttcctaaaaa agtaccttga attacttctt
    75901 ctatagcttg cttatttatt ttaaatccat ttttatctcc ttttaattta attctaccca
    75961 agaaaaatgg atttgctatt tcatatactc ctcctactac aaatagagga gataaatttt
    76021 cttgtctacc tctaatattt ctattcaata cttttattat ttctaaaaat tctactactc
    76081 ttctacattt ctctttatta tcaagttcta tatctccatc cttacctatt ttagataaat
    76141 ctatagtcac tgtatatgta taataactta aatgttgctc tatgttagca agattaggat
    76201 gttctcctat tctatctgct aatcctttat tatttaaaaa gtccatgtca cttctatatg
    76261 gctcaaggga aattgcatta cttaatctta cagtagcttc tctagtcaaa gaacctcctt
    76321 ttttatcttt ctcatctttt ttatttgttt tcatatatcc aaataaatcc atttcttctg
    76381 attctcttat acttatattg tctttaaatt gaatagtacc tttttcttta tcgacaacct
    76441 gtaaattcca attaaacaat tctgctgcta atcgtactat gtcatatctt aaacactggc
    76501 gagatgcaaa tgtatatata tttccatcat ctctacttaa tttttttaat tctgatatat
    76561 tacctatccc ttccccatag tttaaacttt gagcctgtgc tattactgta aatgttaacc
    76621 ctcttttcat cttatttttc ctccttttcg tttggtagtt tgtcttttcc ttcatatttt
    76681 ccagaaatta atccagatat aaaagcatga cctatagatt caaagtctaa atctttttca
    76741 atttctatat ctaaaataaa tgctggaatc tgtttttcag ctgacataaa aactcttata
    76801 attgtatcca taaaatcact tttatttccg actttaactg aatttaaaag tttatagctt
    76861 actccatcta ttttattttt agaattttta ttaacaaaat agtcatgtat atcacaacct
    76921 aaataataaa ttattctcag cttttcatca ttcttctcca aatttttact atccatttta
    76981 tataccccct ttttgtagct atttattaat gctcttaatt ggactactct aaaacaatct
    77041 attcctgata tatttgattt gttgttgctt tccatatcat ttttaacttt atttcttaaa
    77101 atgttattag ttagatgctt caagtcccta tttttcaaga gtaaatctag tacatttgat
    77161 ttaagcttgt aatcatagat actttgtatt tttttatttg catacttgtc tgtaaaaaat
    77221 tttgctaaat aagttggaat attaaaataa tttattttac attttttaga atctatactt
    77281 gctttaaatt ccacaaaaag tatattacga agttgccatt cactttttaa tgtattttct
    77341 tctactatac tttttataag ctgattaaaa ggattttcat ctttggaatc tgaatattca
    77401 cttttttgag cttttaaact attatttctt tcaaaaagct catttataga agtatccatg
    77461 ttaacaaata aataaaactc attttcttca ttaattaaat aattctttct tgtataagta
    77521 gcccctgctg gagtacagaa caatattagc ttacaaatat cacatattga tggtacatac
    77581 gcctgattcc aaaacatatt cctagcatta ttggtgctta ctccaagtgg agcaaaatta
    77641 ctttctgagt attcatctaa tattccctta tataaaccac acatttcaca tacttctaaa
    77701 ctatccatat actcttttat atcttctatg cttttatttt tctttataaa gtttttattt
    77761 atttccttca taattttttc tatagtattt atactagact tactaacttt tttttcattt
    77821 atatccttag ctatactatt taatctatca tttaaatagt ttttcaactt atcatagtca
    77881 ttctcttcta ataaatctgc taactctcca aaatatataa tttgtcttaa ataatcatta
    77941 aacattatct gttttaatcc atctaaatct aatttcgcct tagcaacatt aaaaaaacta
    78001 acttgtccaa aataattatc ttgaactaca tatttataaa gatttgctgt aagcctgtca
    78061 tttattgatt ttattttaaa tatatctata gtctcatcta ctaaagcttc taaactgtca
    78121 aattcatcat aactttttat cttagacatg ctatctagct tctcttttat caggttaaat
    78181 ttttcttcat caaatttttt aatcttatca ttttgttgtt tcacactatc tttaattttc
    78241 ttaattccat ctttgattct atcaggctta gatttaataa aatttatact gtaatcaata
    78301 ttttttttaa ttctttttga tacatcatat tcatccataa agtaattaaa ataatactca
    78361 tgaaagtttt caagaaataa gctatcaaat tcgatataat cttccataat aaatatttca
    78421 gcttgtttat ctgctttctt tattatattt aaaaatccaa ctatacccat attaaaaaac
    78481 caatctcctc tataaactct taacttcatt tatctcacct ccattctaaa atatctacat
    78541 ctccaaatcc ctgagcacgt ctaaaaccaa ttcctagatt gtagataaga tttaaatcat
    78601 caatatcacc tttcaatata aatttacctt tgtaaccatt aatatattga tactctttac
    78661 ctgttatttt tttaaattct cttaagctct ctttaactac tatctttttg agacctatat
    78721 tttcaaattc taactttctt tttaatccat ttcccctata atttttaacc acttcattag
    78781 ttatatagtt tagttcttct atgtatctat catcagttat ctccaagaat tttccttctt
    78841 tagactttac acagattgga gacaatgcat taaatagtac cctttcttct gttaccttct
    78901 tttctcttga taaatcgatt ctaattcttg taagttcata ggctttatat aaacattttt
    78961 tagaagtcat taacccattg tatatatgaa aacccaattc taaatctgga gtacttatat
    79021 ttaatattac tttatcttct ataataaaat tgtcaccttc aatactatac ttttttatat
    79081 aaaccgaaaa tgtaaaattt ttagtttttt tattattctt ttctttataa tagtacatgt
    79141 ttttatagta atcttcatta gattttctta ttgcttcttt tataatactc ataaagagcg
    79201 aattataaga tattggtata caacctgtac taaattcaat tttcattttc ataatatcac
    79261 ctctctcaat taatatatta acactagacc gtgaagtata ttggtccaag tataaattat
    79321 ttgaaaaaat cagtaaattt ttttcatttt ttcaatctct gacaatatat catttctaag
    79381 tctatctggt tctacaacct caacataagc tcccatactt aaaatccaac tttttatctc
    79441 ctcataacct ctcattttag ctttaaacat tatagattta tcatcatatt ctatgatttg
    79501 ttgattatta acccaaactt tttcctttat aattattgaa aatggatgac taatttttat
    79561 aacaacatta atttcttctc ctttataaat tcctatacta tttttactat attcatccca
    79621 actaaaactt ttatctacat tatatttttc ttctaaaacc ttataatctt taattctaca
    79681 taatttaaag tctataaact taaatctttt ttcacaaaaa gctaccatat aagtatctga
    79741 tttataatta aataaaccat atggatgaac aattctttca ctatttcctg aatttaatga
    79801 ataatattct atccaaaatt tgtgttttgt ggtatatgct ctaattattt cattacactt
    79861 atttttttca gacttataat cacaattacg ttgtgcttga actgtaaaat aatccatata
    79921 tgtatcgctt ttttctcctg tatttaatac tgcttttatt ttttctacaa tattattaaa
    79981 ttcatttttg taaatatcat tattatactc taactgtgaa tttatcatat ctagaattga
    80041 aacttcacta tctaataatt taattaatga tatgctatta catttatcta tttcataacc
    80101 accataaatc ccaggtgttg aatttataaa tataccagct tgctctaaat acatcttgta
    80161 actttttatt tgcctttcac ttacttccaa ctcttctgat aattctttgc atttcatttt
    80221 gcctctgctt tgaattaata tcatcattct aagtgtatta gctaatttac taatgagtaa
    80281 ccccccctaa taacattaat tgatattttt attatttatt tctacacata tgtacatata
    80341 acctctttat attttatata agtagaattt ttttataatt ttgacattta ccatattata
    80401 ttttatattt gtacaaaatt ttaatagtta atacaataaa aaatattact gaaaaaataa
    80461 acatctataa ttccagtaat attaattaat tttaaaccct ttttattatc atttaataat
    80521 agtaactaaa aattgactaa atttaacctt atctctttta aataattatt tcttacaatt
    80581 aaaaaataaa ctatttgaac caagaacaag cttcccatta ccaaaacagc tgatgatgct
    80641 acttctatat caaaagaatt tttcagtgca gtaagggcaa atattgaatg tataacagcc
    80701 actacataag gaaacaaaaa tactattcca atctcaattg ttgaagcttt tttaatctca
    80761 ttatatgtca tccccaattt atttaattgc tcatactttt tcttatcaac ttggcaatcc
    80821 atataaaatt tattatataa aaaactactt gtagtaacaa agaaaattaa acctataaat
    80881 attgctaaaa atataaatgt agaatatatt aatttcccag ttcctatcat taattcttta
    80941 gagaaaaaca aatatggttg catccctgac tcattattaa atttattttc aaattctctg
    81001 catatctcta tagttttact tgaatcctct acttcaaatg cagtaaatct atccactata
    81061 aattttgatt ctatactatc ataaaaatta tcctttacca catacatatt tttgtaatat
    81121 gctggcataa cacattcttc cacttgtttt gccaccttta aacctctatt ccctacaata
    81181 atattttctt tgacgttatt atttcctggt aaacttgatg ataataatga tatgcattca
    81241 ttatcttcaa aacttatagg cttaacccct atagtttctg taatttctaa gtacttagat
    81301 tctttcataa ttctgacatt aaaatcactt ttcttgggaa atacagtttt aaattcacca
    81361 ttagtccttg tatagtctac attctctttt tttagattat tttctataaa catcattctt
    81421 tccttgtctt tttctttgct atcaggcttt ttagggtcat tatgcaatgt cattgttgaa
    81481 taatatattg tgttaggata tattaaatta atttgacgct ctacatcttt ccaaaaggaa
    81541 taaactgtac ctatagcagt aaatgctact gcagaagtta tagttatcaa gaaaaatatt
    81601 ctagcattat cctttacctt ataaatcaag ttcgaaatcc aaagcatatt tgtatttttt
    81661 ctataaaatc tcttgtttag ttttagcttc ttcataaaaa atacactaaa ttgagaaaag
    81721 aacaagtaag ttgctactat agtaagggat gtaactggaa ctaagcgcag atatgttgta
    81781 tattggtcgg ttgaggtaat agcattataa tacgctatta taaacaaaca aatacttaat
    81841 atagcaagca ttagtgatgt tttaggctct ggttttggag tttttgtacc tttaagtaac
    81901 ttcaacacct gattttcttt aactataaaa taagtaaata ttgatgttaa tacagctaaa
    81961 atcataaaat agattaaagt agacaatatc gcattaaatg gtatatagaa ttttaatggc
    82021 gataattcta aaaaagtaga cataaccact aaaaatatct ttgcaaagat taagcctatt
    82081 attattccaa ttacagaaga tactatattt attatcatat tttctataaa gattaatttc
    82141 attacttggc gttttgatat acctgtaata taaagtattc caaattcttt atatctactt
    82201 ttaagaaaca cactaactga ataaaacaca aagaagaata agaatagata tgcaattatc
    82261 ttactcatct tgagaccttc ttttaaataa tcaggaaatt gagatacatc taaatctgga
    82321 tgacttgtaa acataataaa agaaaatagc agtgaagatg atataacaat acttgctaag
    82381 tatcctaaat acgctttaat atttcttgaa atattgttaa tagcaaactg ttttatattc
    82441 attagttatc gcctcccaat aatgtaagta catccattat ctcttgataa aactgctctc
    82501 tactactacc cttatatatt tcattatata tagacccatc ttttatgaat aagattctac
    82561 tacagtagct cgctgctaat gggtcatgag ttaccatcat agtagtaact ttattttctt
    82621 tatttatctt ttgaaatagc tccattacaa cctttgatga ctttgaatct aaattacctg
    82681 ttggttcatc tgctaaaagt attgatggat tgttaatcaa tgctctagca atagcagtcc
    82741 tctgagcttg accacctgat acttcaaaag ttctttttcc taataaatct tttataccta
    82801 atattgtaga tacttcattc aatctctcat cttgctcttt tacactgacc ttatctaatg
    82861 tcaatggcaa tactatattt tctcctatag ttaatgtatc caataaatta aaatcctgaa
    82921 atacaaaacc taactctctt cttctaaata atgcaagttc ctcacctctt aacaataaag
    82981 gattctttcc ctttaattcc atagttcctg tagttggctt gtcaatagta gaaatcatat
    83041 ttaaaagagt agtttttcca ctaccagatg gacccataat tcctacaaat tctccatctt
    83101 ctatactaaa atttatgtca tttaatgcgt tgaaaattat ttttttacca tatactttac
    83161 ttaaattatt tacagttaaa attttcatta tttaactatc cttcctaatt taatttttga
    83221 aataacatat atataattgt taattataat ttatcttagt ttaattttta tagataataa
    83281 atctattttt tctatacact aattatgtta gtaaaagcta atactgcaag tactacaccc
    83341 atgattattc tgtatacagc aaacactctc attggttttt tctttaaaaa attcacgaat
    83401 ttttccataa ctattattga tacaataaaa gctactataa acccaactac caaagcaatc
    83461 cacaatgttg gtgtcattat tgaataatca aattcaaata aatcttttcc tgaagaagcc
    83521 accatagctg gtactgctaa aaagaaagaa aactcagctg ctgtaggaga attaagtcct
    83581 gctatccatc cacccataat agtagatgca ctccttgaca tacctggcca tgcagacaaa
    83641 acttgtaatg tcccaacctt taaagcttgt attggagtaa ttttatctat attatctgtt
    83701 gaatgattct tttttctaaa caaagtttct actactaata ataaaatccc acccactata
    83761 aatcctataa ttacagattg taaattaaac aatgatttta tattatcata aaatagaact
    83821 cctataatcg caaatggtat acaacctata attacattca ttccaaatct aaaaccaatc
    83881 tttccttctt ttccgccagt aaatataaat ttaaaaaatt ctataacgct atcttttatc
    83941 tttttccaat aaagtactac tactgctaat atagccccaa gttgtattac aacctcaaac
    84001 atttcagcaa attgcccctt aaaatctatt aaattaccaa ccaaaatcat atgacctgta
    84061 gatgaaactg gaataaattc tgtaaacccc tctactatag ctattatgac tgatttcaat
    84121 ataaaaataa catctaaact catcaataaa catccttcct tttttacaaa atttcttttt
    84181 ttatgttatt tctctcatgt tatttcttat attaattgta atttagatta gaaaatttaa
    84241 ctatctgcaa acattacaca aaccttacat ctatgtaagg tttgcaaata aactctctta
    84301 aattttgatt atttaactat gaatatttca tttaataaat tgtataataa aaaagtacaa
    84361 ttaactaaaa accctattgt aacagataat ttacatcttt atcaaactat ttacatcttt
    84421 attaaagtag attgaaactt ctgtaccttt tcctacttct gattttatac ttactttatg
    84481 acctaaatca tcacatacct tttttactag ataaagaccc atacctgtag attctccaaa
    84541 cttacgacca ttttctcctg taaaaaatgg gtcaaatacc ctttttaaat ctttagaaac
    84601 aattccaatt ccttcatcta ttatacttaa aattacatat ttattatttt cttcagattt
    84661 tatagttagg tagttactat aatctttaga atatttcact ccatttgtaa ttaattgacc
    84721 tattataaat ttcatccatt ttacatctgt ataaacataa gtaaatttgt ctacttgaat
    84781 cttaggaatc attccacttt ttataaataa ccttttttct tcatttacaa tactagatac
    84841 aatttcattt aatttggctt tttcaactat aaagtctttc tgaaaattat ctgcccttgc
    84901 aaaatgcata gccatatcta accctttatt taatctatct atttcttttc ttatattttc
    84961 agttacttct tctccctcat aatcttgaag ctgaaggctt ataactgata ttggtgtttt
    85021 catctgatga atccattggt tgatgaaagt taaatgttca ctatgtatct ttttatgctc
    85081 ttgtatcatt gattgataca atttatgttg ttgttttaaa ataactgata tatttctccc
    85141 caagaatgag cttcctaaat acaaaaatga ctcctccaaa gaatgtattc ctttttcaaa
    85201 cagattatat acttctctat ttttataata cttatatatt aaaaaacata acaatataaa
    85261 agaactaaat aaaagaatat ataaactctc tccaaaacta ataaacttca taagattaca
    85321 gtaaataatt gtcataacta tgcctatata atatataaaa atataacctt tataatcttt
    85381 taaaaataac ttcatacttt ctccttacca attcttattt aatttatatc ctaaccctcg
    85441 gacagtctga acagcattat ctatacctaa ttcctcaaat cttttcctta tcctgctaac
    85501 atttacattt aaagtatttt cttctacaaa ctcaatatca tcccatatct tttctaaaag
    85561 ataatctcta cttactactt ttgggtatct cttgataaga cattcaatca gtataccttc
    85621 ttttttagtt ataattagat tttcaccatt taattctagt tctaaccttt ctggataaaa
    85681 ctttaatcca tctagctcca ctattctttc gtctatttta ggtgaatatt caccatatac
    85741 ccttcttata tgactcttta tttttgccat tactacatca tagtaaaaag gttttgttat
    85801 ataatcatct gctccacttt ctaaagctct aatttggtct acattgccat ctctagctga
    85861 tataaaaatt ataggtaaat tcgactgttg tctaatacat ctacaccagt agaaaccatc
    85921 aaatttaggt aaatttacat ctaatagaac taagtgtggc ttaaaacttt caaatgtaat
    85981 cattatatca tcaaagttct cttcaacaat cacttcaaat ccatatttct caatataatc
    86041 tttaagtaat gtacttattg atatatcatc ttccacaata tatactttat aactcatata
    86101 attcctccta atatacctat ttactcatca tataacagtc caattttaat atattataat
    86161 ctttttacaa tatataaatt taatcatatt attaacttca caattactga cttcataagt
    86221 ataaccatat tattagacat cttaataaaa ctctataaaa aagtatcttt ttatgattaa
    86281 ttaatcatta ttatcaacta atcattctaa aataaattga aattactaat tcaaaataga
    86341 aaaagttacc ttaataaggt aacttaaact tttattatat ttcttactta gtattatttt
    86401 agcatagttt ctacttagca ttatagatta attcttttat attcactttt ttctcttgta
    86461 acttattttt tattaagaag tcttgtgtat cttcaagcga agatatatct ttatctgtta
    86521 tatctaaact aaaatcatac caagcatata attctttagt ttcttcaaga cttaaatcaa
    86581 cttctttagc tactttttcc atagcttcat caaaattatt atttacatat tctaaagttt
    86641 ccttttcaac cttcataaat ctttcaacta tttctgggtg tttctctgca aagtctgtgc
    86701 taacagcagt tactattata ccatctacta aaccttcacc atttgctaca agtttactac
    86761 ctgacttcat agctttaaga gctgctggac cagcaatcaa tgcaacatct acgctaccat
    86821 cagaaagagc tgctgatgct tctggaatac ccatattaac aaattccaca tcatccatac
    86881 ttaatccttc tttatcaagt gctgaaatca atacctgatg taatatagtc ccttttggac
    86941 ctgccacctt cttacctact aaatctgctg ctgattttat tgaattatta ttagtcaaaa
    87001 tcataaatcc ttttggtgac ctgctataag tgtttaatat ctttaactct actccattag
    87061 atgcagctat taaagctgac gtaccaccta gtgcatgtaa gaagtctatt tctccagcag
    87121 ctaaagcctg tgtttgctct ggacctgtag taatttcgtg aaagttaact tttatattat
    87181 cctttttaaa ttcctttcca aataaatcat cctgtttctg aattatagaa ggaacattta
    87241 atggtgattt tacataagtt aaatttattt ctgaaagttt ttctccatta ttttttttag
    87301 cacatccagc taaagatgct gttgcaagtg ttaatatcgt tatacctatc aatagctttt
    87361 ttaatttcat ttactctact ccttaaaata ttattaattt ctttaaatgt ctttatttga
    87421 tttcattgtc attaaaatat ctttttttaa tttaatatag tactctgaag ttaaatctct
    87481 attataacca tcttctatat taaattcttt tatttttctt tcgtctgtga aaacaactat
    87541 ttttttgcct atctttaacg cttcatcaat atcatgagtt acaaatatta cgcctttatt
    87601 agttgactta tgtattttta taacttcatt ttgcatatca atccttgtaa aataatctaa
    87661 tgccgaaaaa ggctcgtcca taagcaacat atctggattg aaagaaagag ctctagctat
    87721 gcttactctc tgtgccatac ctcctgacaa ttcatttgga taagagtttt taaatttctg
    87781 caagttcatc atcttaagat atttttctac atcaatatta cttttcctat tatatttatt
    87841 tgatttgttc aaattatcat ttgtttctaa gcttctaaaa ttatcattta taattttacc
    87901 aataattctt ttattgtgtt taaattttct aagcaccttg tttaaaatag tattttttct
    87961 ttgattatga aaagctatat tttcacttac gtttaaccaa ggcattaatc tgctctcctg
    88021 aaaaacaaaa ccaacattag gtctatactc tttattatca ttaataaacc taatctcacc
    88081 actatttata ccttcaagtg atgcaattgc tctaagcaag gtagtctttc cacaaccact
    88141 ttcacctaaa attactgtta tatggtttct atctatgttt aaagagatat cactaagaac
    88201 tacatgttct tcattgtcaa ctttataact tttatataaa tttctaatta tatatccaga
    88261 gtcacatact aaattattca tatgcttcca ccatctttcc cttacttacc ttcttcacaa
    88321 taatcgaaaa tatgtaatct gtaattattc ctaaaagtcc aattacgatt atacctacaa
    88381 taacaacatc tgttctagac atttcttttc catcagatat caagtatcca agaccagatg
    88441 aagctgctac aagttctgcc ccaataattg ctctaaagct atatccaaga gctaacttta
    88501 acccaacagc aatatcaaga atggcatttg gtattattat tttaaatatt atctgaagct
    88561 ttgaaaactc aaatactcta cccacttcaa tcagttttga gtcacaattt ttaatccctt
    88621 tcaatgtgct tgtaaagatt gggaaaaagg atgctaaaac tataattata atttttgatt
    88681 cctctcctat accaaaccat aatataagca ttggtattaa agcaagggga ggtgtattcc
    88741 ttaaaaaatt tattagagat ttaaagtatt cgtatactcc actataaatt ccaaagaata
    88801 tccctaaagg cacacctata gcagtattta ttgcaaaacc tatcaaaact cttttcatac
    88861 ttgcatatac attaataaat attgaaccat cacttatcat atttaaaaat gtactatata
    88921 ccttttcggg tgatggtaat atataatcac tccaaatacc aagataattt gtaattttcc
    88981 ataataaaag aattataaaa aatatcagaa cactctttac tagccttaaa aacttattat
    89041 taaatttatt gaccatttac aaaccatctc cattattaca accatcttta caataaaaca
    89101 tttctactat gtcatactta tcttcaaagc catttcttat tttttgtatt tcacttaata
    89161 tttcgtcttc acttgatact tcaagaactt tttcaaaagt atctttgaaa tatcctaatg
    89221 gaattggtga ttcatctatt tttccaagtg attttacacc ttcttgttca gcaaactctt
    89281 tccatgtata aaacatagca ttacccttat ccttaaactt ttcacttgca aagtctctta
    89341 attgtgtaca tggacaggtt actaccaaca tatcatcttt actttttata tatttatcgt
    89401 ataaaaccct acaagtcctt actaatattg gttctatcat aggtacttca aaattattta
    89461 tcaaattatt tctttttaaa agacttatac tttctggaca tctacaatcc aacattgtat
    89521 tttcagtctt ttttgtatac tctttgtact gcttttttac atattctaac tgtggttcac
    89581 attctgctat tatgtacccc ttttttatca aatttgcttt aataaggtga aacttttctc
    89641 catacattct ttctgccact ggatttatta gaagatattt attcataacg cctcctatta
    89701 tttaatattg aatattatta tcaattaata gtttaaatga gcacttaaat tttgtcaata
    89761 tattaatttt tccttaataa aacaattttt atctacattt tttaa
//
LOCUS       DAMKPG010000004        88938 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMKPG010000004 DAMKPG010000000
VERSION     DAMKPG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 88938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.004"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..2492)
                     /locus_tag="PC621_000310"
     CDS             complement(<1..2492)
                     /locus_tag="PC621_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF4107501.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTTEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGST"
     gene            2706..3404
                     /gene="yunB"
                     /locus_tag="PC621_000311"
     CDS             2706..3404
                     /gene="yunB"
                     /locus_tag="PC621_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF4107502.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(3616..4623)
                     /locus_tag="PC621_000312"
     CDS             complement(3616..4623)
                     /locus_tag="PC621_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF4107503.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            4851..5735
                     /locus_tag="PC621_000313"
     CDS             4851..5735
                     /locus_tag="PC621_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4107504.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            5937..7487
                     /locus_tag="PC621_000314"
     CDS             5937..7487
                     /locus_tag="PC621_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF4107505.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            7516..8151
                     /locus_tag="PC621_000315"
     CDS             7516..8151
                     /locus_tag="PC621_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF4107506.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(8262..8522)
                     /locus_tag="PC621_000316"
     CDS             complement(8262..8522)
                     /locus_tag="PC621_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF4107507.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            8739..10022
                     /gene="hflX"
                     /locus_tag="PC621_000317"
     CDS             8739..10022
                     /gene="hflX"
                     /locus_tag="PC621_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF4107508.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            10030..10707
                     /locus_tag="PC621_000318"
     CDS             10030..10707
                     /locus_tag="PC621_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF4107509.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            10793..11236
                     /locus_tag="PC621_000319"
     CDS             10793..11236
                     /locus_tag="PC621_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF4107510.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            11542..12186
                     /locus_tag="PC621_000320"
     CDS             11542..12186
                     /locus_tag="PC621_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF4107511.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(12272..12649)
                     /locus_tag="PC621_000321"
     CDS             complement(12272..12649)
                     /locus_tag="PC621_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF4107512.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(12649..13035)
                     /locus_tag="PC621_000322"
     CDS             complement(12649..13035)
                     /locus_tag="PC621_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF4107513.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            13214..13969
                     /gene="nadE"
                     /locus_tag="PC621_000323"
     CDS             13214..13969
                     /gene="nadE"
                     /locus_tag="PC621_000323"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF4107514.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            14052..14771
                     /locus_tag="PC621_000324"
     CDS             14052..14771
                     /locus_tag="PC621_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF4107515.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(14939..15943)
                     /locus_tag="PC621_000325"
     CDS             complement(14939..15943)
                     /locus_tag="PC621_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF4107516.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            16209..17105
                     /locus_tag="PC621_000326"
     CDS             16209..17105
                     /locus_tag="PC621_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF4107517.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            17243..18505
                     /locus_tag="PC621_000327"
     CDS             17243..18505
                     /locus_tag="PC621_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107518.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            18581..19774
                     /locus_tag="PC621_000328"
     CDS             18581..19774
                     /locus_tag="PC621_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF4107519.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            20403..21197
                     /locus_tag="PC621_000329"
     CDS             20403..21197
                     /locus_tag="PC621_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF4107520.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            21291..22631
                     /locus_tag="PC621_000330"
     CDS             21291..22631
                     /locus_tag="PC621_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF4107521.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            22768..24000
                     /locus_tag="PC621_000331"
     CDS             22768..24000
                     /locus_tag="PC621_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF4107522.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            24040..25182
                     /locus_tag="PC621_000332"
     CDS             24040..25182
                     /locus_tag="PC621_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF4107523.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            25193..25987
                     /locus_tag="PC621_000333"
     CDS             25193..25987
                     /locus_tag="PC621_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF4107524.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            25998..27020
                     /locus_tag="PC621_000334"
     CDS             25998..27020
                     /locus_tag="PC621_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF4107525.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            27119..28495
                     /locus_tag="PC621_000335"
     CDS             27119..28495
                     /locus_tag="PC621_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF4107526.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(28827..29171)
                     /locus_tag="PC621_000336"
     CDS             complement(28827..29171)
                     /locus_tag="PC621_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107527.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            29659..30669
                     /locus_tag="PC621_000337"
     CDS             29659..30669
                     /locus_tag="PC621_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF4107528.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            30805..31293
                     /locus_tag="PC621_000338"
     CDS             30805..31293
                     /locus_tag="PC621_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4107529.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            31469..33397
                     /locus_tag="PC621_000339"
     CDS             31469..33397
                     /locus_tag="PC621_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4107530.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            33572..33982
                     /locus_tag="PC621_000340"
     CDS             33572..33982
                     /locus_tag="PC621_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF4107531.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            34004..34306
                     /locus_tag="PC621_000341"
     CDS             34004..34306
                     /locus_tag="PC621_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF4107532.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(34522..35214)
                     /locus_tag="PC621_000342"
     CDS             complement(34522..35214)
                     /locus_tag="PC621_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF4107533.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(35240..36427)
                     /locus_tag="PC621_000343"
     CDS             complement(35240..36427)
                     /locus_tag="PC621_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4107534.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            36763..38640
                     /locus_tag="PC621_000344"
     CDS             36763..38640
                     /locus_tag="PC621_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4107535.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            38952..40358
                     /locus_tag="PC621_000345"
     CDS             38952..40358
                     /locus_tag="PC621_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF4107536.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            40386..41234
                     /locus_tag="PC621_000346"
     CDS             40386..41234
                     /locus_tag="PC621_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4107537.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            41895..42587
                     /locus_tag="PC621_000347"
     CDS             41895..42587
                     /locus_tag="PC621_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4107538.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            42584..43519
                     /locus_tag="PC621_000348"
     CDS             42584..43519
                     /locus_tag="PC621_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4107539.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            43667..44590
                     /locus_tag="PC621_000349"
     CDS             43667..44590
                     /locus_tag="PC621_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107540.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            44574..45344
                     /locus_tag="PC621_000350"
     CDS             44574..45344
                     /locus_tag="PC621_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107541.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            45354..46136
                     /locus_tag="PC621_000351"
     CDS             45354..46136
                     /locus_tag="PC621_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107542.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            46513..47049
                     /locus_tag="PC621_000352"
     CDS             46513..47049
                     /locus_tag="PC621_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF4107543.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            47081..47470
                     /locus_tag="PC621_000353"
     CDS             47081..47470
                     /locus_tag="PC621_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF4107544.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            47507..47893
                     /locus_tag="PC621_000354"
     CDS             47507..47893
                     /locus_tag="PC621_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF4107545.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            48020..49462
                     /locus_tag="PC621_000355"
     CDS             48020..49462
                     /locus_tag="PC621_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF4107546.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            49759..50574
                     /locus_tag="PC621_000356"
     CDS             49759..50574
                     /locus_tag="PC621_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4107547.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            51175..52359
                     /locus_tag="PC621_000357"
     CDS             51175..52359
                     /locus_tag="PC621_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107548.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(52736..53845)
                     /locus_tag="PC621_000358"
     CDS             complement(52736..53845)
                     /locus_tag="PC621_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4107549.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            54308..55501
                     /gene="nifV"
                     /locus_tag="PC621_000359"
     CDS             54308..55501
                     /gene="nifV"
                     /locus_tag="PC621_000359"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF4107550.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            55526..57451
                     /locus_tag="PC621_000360"
     CDS             55526..57451
                     /locus_tag="PC621_000360"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF4107551.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            57473..58468
                     /locus_tag="PC621_000361"
     CDS             57473..58468
                     /locus_tag="PC621_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF4107552.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            58795..59268
                     /locus_tag="PC621_000362"
     CDS             58795..59268
                     /locus_tag="PC621_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4107553.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            59302..59796
                     /locus_tag="PC621_000363"
     CDS             59302..59796
                     /locus_tag="PC621_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4107554.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            59893..60414
                     /locus_tag="PC621_000364"
     CDS             59893..60414
                     /locus_tag="PC621_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4107555.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            60603..60914
                     /locus_tag="PC621_000365"
     CDS             60603..60914
                     /locus_tag="PC621_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF4107556.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            60942..61628
                     /locus_tag="PC621_000366"
     CDS             60942..61628
                     /locus_tag="PC621_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4107557.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            61860..62744
                     /locus_tag="PC621_000367"
     CDS             61860..62744
                     /locus_tag="PC621_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF4107558.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            62843..64018
                     /locus_tag="PC621_000368"
     CDS             62843..64018
                     /locus_tag="PC621_000368"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF4107559.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            64146..65000
                     /locus_tag="PC621_000369"
     CDS             64146..65000
                     /locus_tag="PC621_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107560.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            65044..66279
                     /locus_tag="PC621_000370"
     CDS             65044..66279
                     /locus_tag="PC621_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF4107561.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            66380..67321
                     /locus_tag="PC621_000371"
     CDS             66380..67321
                     /locus_tag="PC621_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF4107562.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(67550..67903)
                     /locus_tag="PC621_000372"
     CDS             complement(67550..67903)
                     /locus_tag="PC621_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF4107563.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            68199..69110
                     /locus_tag="PC621_000373"
     CDS             68199..69110
                     /locus_tag="PC621_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF4107564.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            69370..69804
                     /locus_tag="PC621_000374"
     CDS             69370..69804
                     /locus_tag="PC621_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107565.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            69827..71362
                     /locus_tag="PC621_000375"
     CDS             69827..71362
                     /locus_tag="PC621_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107566.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            71446..72813
                     /locus_tag="PC621_000376"
     CDS             71446..72813
                     /locus_tag="PC621_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4107567.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            72848..73072
                     /locus_tag="PC621_000377"
     CDS             72848..73072
                     /locus_tag="PC621_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF4107568.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(73522..73920)
                     /locus_tag="PC621_000378"
     CDS             complement(73522..73920)
                     /locus_tag="PC621_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF4107569.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(73923..74324)
                     /locus_tag="PC621_000379"
     CDS             complement(73923..74324)
                     /locus_tag="PC621_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4107570.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            74683..75669
                     /locus_tag="PC621_000380"
     CDS             74683..75669
                     /locus_tag="PC621_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107571.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            75653..76564
                     /locus_tag="PC621_000381"
     CDS             75653..76564
                     /locus_tag="PC621_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107572.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            76677..78245
                     /locus_tag="PC621_000382"
     CDS             76677..78245
                     /locus_tag="PC621_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4107573.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            78320..79345
                     /locus_tag="PC621_000383"
     CDS             78320..79345
                     /locus_tag="PC621_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107574.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            79338..80282
                     /locus_tag="PC621_000384"
     CDS             79338..80282
                     /locus_tag="PC621_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107575.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            80641..82299
                     /locus_tag="PC621_000385"
     CDS             80641..82299
                     /locus_tag="PC621_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107576.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            82280..83296
                     /locus_tag="PC621_000386"
     CDS             82280..83296
                     /locus_tag="PC621_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4107577.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            83511..84140
                     /locus_tag="PC621_000387"
     CDS             83511..84140
                     /locus_tag="PC621_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107578.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            84275..84601
                     /locus_tag="PC621_000388"
     CDS             84275..84601
                     /locus_tag="PC621_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4107579.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            84617..85906
                     /locus_tag="PC621_000389"
     CDS             84617..85906
                     /locus_tag="PC621_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4107580.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            85930..86259
                     /locus_tag="PC621_000390"
     CDS             85930..86259
                     /locus_tag="PC621_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4107581.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            86407..87735
                     /locus_tag="PC621_000391"
     CDS             86407..87735
                     /locus_tag="PC621_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF4107582.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            88052..88912
                     /locus_tag="PC621_000392"
     CDS             88052..88912
                     /locus_tag="PC621_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF4107583.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
BASE COUNT        33927 a        10078 c        16058 g        28875 t
ORIGIN      
        1 gtagaaccgc caccattatc acctgtgtta ttattgttat tattattttc ttctggttgg
       61 ttattgtctg gtttttttgt atcttcagat gatggttgag aacttacttt agtaggagca
      121 gtaccttcaa taaatgctgc tttagagcca gaagattgct ttcctccatc ctctaaatta
      181 atttttgtaa aatatacatt tttaggaact tcaaattcag taaccgttaa attagcatgt
      241 attttttcca taattttttc ccatagtttt gcagtactag tacttccatg tggtacttct
      301 ctacgtttta tagtgttacc agaatcatct tttctaccag catcatctcc aatataagtt
      361 gcaccaacat aataaggagt gtaaccgaca aaccaagcat catatgcact atttgtagta
      421 cctgttttac ctgctacagg cattcccttt ggaagtgtag caacaccacc agtaccttca
      481 gtaattacgc tttctaacat atctgtaaca acataagcta cttcagggtc tacaacttta
      541 tgttcttcag gagcattttt aactaataat tgaccatcaa aggtagttat agtagtaaaa
      601 atagaaggtt ctacataaac accttgattt gccaatgtac cataagcagc agccatttga
      661 agtggagtaa taccattcgc cataccacct agtgtcaatg atgggaattt tctatcactt
      721 gcttcagatg aacctgagtg attattcttt aaagttgtta taccaaagtt ctttaagtaa
      781 tccatcatta tatctacaca ttcatcataa gagtctccaa gcatttcagt agttttgaca
      841 gctatcgtgt ttgaagattt agcaagtgct ttacgtaaac tcatagaacc atgaccagca
      901 gtagtagttc ttggattcca tttattattt tcttcaaact tatatcctcc tcgagaatca
      961 cttagagcag ttgattgtgt tatttttagg gtatcaatag caggtgtata tattgctaaa
     1021 ggttttatag aagaacctgg ctgatgaggg ttagtagcac gattcaaagt ttttctacca
     1081 cttatatttc ttcctccagc tagtgctctt atttcaccat ttttataatc taagattacc
     1141 attgcagctt gtggttggct aactccatca gggccgacca tatgtcctgg gaaattgcta
     1201 tttctatcaa attcttcttc taaagcatct tgcattttag ggtctatagt tgtgtttaca
     1261 attagaccac cattgtagaa taagttttga gcttcatcat ttgtatgtcc ttgttccatt
     1321 aaagcattta taacttcact ttcgataagg tcttctacag atgaagaaac tttatctgat
     1381 gattgaggca attttatttc tattttagct ttgattgctt catcatattc actttgtttt
     1441 atatatttca attctaacat tttctttaaa acaatttctt gtcttttttt agcttcaggg
     1501 ttacttacag cctttctaac aactaatgta cctgctttta attgtctata agtatcatta
     1561 gatataagct cccaagattt tattttttca atcatatcaa agtccacttg agtagggtca
     1621 tctaaatcat catcagttgt gttaacaaaa aataataatt tattttctaa atcctcctta
     1681 gtttcatttc cctctaactt agaagtttta taggctgaaa atcttgatgg atttttagtt
     1741 gaaccagcaa gaagtgctga ctctgctaga gtgaggtcag ctgcagactt atcaaaataa
     1801 ccacgtgcac cagcctcagc acctgctaga cctctaccaa cgaagaagtt atttaaataa
     1861 gtttctagta ttttatcttt acttactgtt ttactcatct catgtgcata gtaaatatcc
     1921 tttattttac gaggtatagt ttgctgttca gttgttaaca gcattttgga tacctgcata
     1981 ggaatagtac taccaccttg cttagtacct gtaagggtct ttaatactga cctaaataaa
     2041 cctttaatat cgacaccctt atgctcataa aaacgctcat cttctattgc aattacagca
     2101 tgttgaagat aaggggaaat cttatctaat ggaacgggtg tctttttatt tacacttgga
     2161 gctgtagaca atgtttttcc attagcgtat ttaatagtag ttgtttggtt cgtctgttta
     2221 tctaaaacag cttctgtaac tggagttaca tctcttagtg acgcaaatac tagaccagca
     2281 gtacctgctg taccaacaac tagtaaaact aaaaatacta tcccaaatac tcttagtttt
     2341 ttaaatttat cgcttttttt agattttttc ttgttttttg ttgaacgagt cttgttagct
     2401 gaagttctat taattggttt atttgtatta ccagatgagc taactttttt tctcctgatt
     2461 ttatttccat ttttatcatt atcgttattc ataagatttt aacctcctta cttaatgcaa
     2521 tattataaca caaataggag tagttttgta attaatagaa aaaatgaatt tgctataata
     2581 ttctgtacat tgtatatgta taaacgtaat atattaaaaa gtctatattt ttattttatt
     2641 tatttttgac aaatattata aaaattatgc caaagctaca tataaataaa taggagggga
     2701 taattttgaa tagaatagta gatagaaaaa caaaaaatga atttagaaaa atcatagcta
     2761 tttttttagt aattcttttc ttgtctgtat ttataggcag ttttatatat atagataaaa
     2821 ctttaagacc gacaataaca gttttagcag agactaaagc tcttgaacta gcaaataggt
     2881 cgataaataa agctgttgct gagatggtag aaggaaaaat aaattatgaa gatttaatgg
     2941 atatacaact tgataataat ggtaaaataa ctatgataca agcaaacact ataatgatga
     3001 atgaaattgc atctgcaata gctttagaaa tacaagatga attaaaaaag gataagacag
     3061 caagttctta catacctata ggaacagcat taggaagtcc tatattagct aaatacggtc
     3121 ctaaacttga agtgtctatt gaacctatag gaacagtatc agtaaacttt aaaacggaat
     3181 ttgagtcttc aggtataaat caaactagac atagaatata tttagaagct caaacacaag
     3241 ttaaagtagt aataccactt ataacttcaa caaaacaaat taaagctcaa atacctattt
     3301 gtgaaactat aatagtaggt gatgttccag aaagttatgt aaatattcca gaaaaaaatc
     3361 taggaaatgt tttacctaac actggaaaga ataccaacaa gtaatataaa gttgtatatt
     3421 tttaaataaa ttcaaaataa tagtttataa ataaaatttt tggaaataat attaatataa
     3481 ttttaataaa aatttatatt ttatactatg taaaatatct aatatatttg gtctgtaggt
     3541 taaataaaag tacctttaga ccaaatatat tattaataac ttaataacat cttaaaactt
     3601 atttatttat attatttaca acatccattt aataaataca ccataaccta cagctgggtc
     3661 attttcaatt gcatgtgaca ctgctccgat tattttatta tcttgaacta ttggagtacc
     3721 actcatacct tgtacaatac caccagtttg agctaaaagc tttttatctg taattttaat
     3781 tttaaaactt ttactttctg gatgtttttg gttttctata tctataattt caatattata
     3841 ttttttacac ttattaaagt tatcttgtaa aattatttga gcttttccta atttaacttc
     3901 atctaaagat gctactttat atttttttaa attggaaata tcaaagttat taatttgacc
     3961 tcttattcca aaatcagtat tttctgtaaa gctaccaata atattttctc gttttgcatt
     4021 tatacatcca acactacctt tataagattt ttctatatta aggtgcttag ttgtagaaat
     4081 tgaacctgtt ttaataggta tttttcttgc attaccaaca cttattggat gacctacagc
     4141 tccaaactct gatgtatctg gattgatata cgttaaagta gcaaatccag aaagtaaatt
     4201 gttaaaacta attttttcta atatgtgttt ggtagttttc attgttataa gttgtccacc
     4261 tctatttaat aaaatagata cttgtttttc ttctggtaaa gcatttaagg tattggcaaa
     4321 gtcactatat ccatcaactt cagaattatt tacctttact atagcatcac ccaatctaaa
     4381 tggagatgct tctccaggat ttctaactat gatatttttt aattctgcgt caatttgaag
     4441 tacgtttcca attggtatta aatattcagg ttcatttttt tgtaaatttt gtgcataaat
     4501 taaattattt gagaaaaaat ataataaaaa taaaaatgtt aaaacaattg taaattttga
     4561 cttagctaag ttggtttttt tacttaaagt attattttta aaatgaaaat ttttaagttg
     4621 cataaaacac ctctttctta ttaaattatt ttaataatag aattgaagtt ttcttgctgc
     4681 ttctatatta tgttgtgcaa gaattatgtt ttgatgcttg gaaaactgac tcaataacac
     4741 cttagttttc ataaaaataa ataaaataaa tagtgtatat gataaaataa atacatattc
     4801 atataaataa aaataaacaa taactgtcta gcataagaaa ggagcaaaaa atgaaaaaat
     4861 atagattgtt agttacttgt ataattgcca tgggaatcat aattatagga acatcaaatc
     4921 cagttagcaa tttttacaag actacaaatt tgattagttc aggaaatatc acaaatgaaa
     4981 aagcagataa agatgaaaat tcaaacaaaa ataataaaga taaaaatgaa gacaaatcta
     5041 attctaaaag taataaagat aagtcatcag aggacaaatc gtcagataaa aaaaataaaa
     5101 ttcaaaaaaa taaaaaactt ttaatttgta tagacccagg gcatcaagga aaaggagata
     5161 gcaatttaga accagtagct ccaggttctt cctctaaaaa agcgagagta tcttcaggaa
     5221 cagagggtat tgctacaaaa aaaccagaat atgttctaaa cttagaagca tctcttgttt
     5281 taaagagtat attagaatct aaaggttata atgtaataat gactagagag acacatgatg
     5341 taaatataag taattcagag agagcaatac ttgctaatga taaaaaagca gatatggttg
     5401 taagaataca tgcagatagt ttaaataatt cttcaaaaac tggagcatct atattgattc
     5461 ctgaaaaaga tggaaaatat acagctccta tatatgaaga gagtaataaa tgtgctgaat
     5521 ttatcaaaca gaatatggaa caatcaggta tacaaatcaa tggaatcgtt caaagaggtg
     5581 atttgactgg atttaactgg tcaaaagtac cagccgtatt agtagaaatg ggatttatga
     5641 gtaactataa tgaagaccaa atgatgtcaa atccagacta ccaaagaaaa atgatgcagt
     5701 gtatagctga tggattggat gcgtatttta aataagatat gaatagagaa aaagttaggt
     5761 tggtattatt ttcctaattt ttatctctat tttttattgt acataatgca aagaagaagt
     5821 ttattttagg ttaaattgtc gtaaatataa ctaaaaatat tgggaatact tgatttaagt
     5881 ttaaaaagaa tatattatag tatagaatgt gtaaaatttt tttgagaggt gttgaaatgc
     5941 tagtagtaga aaatgtgagt catggatttg gtgcgagaac aatattagaa aatgtatcct
     6001 ttagacttag aaaaggtgaa catattgcac tagttggtgc taatggagaa ggaaaatcga
     6061 gttttttaaa tataataaca aagaagctta tgccagatgc tggaaatata aaatggtctt
     6121 caagagctac agtaggttac ttagaccaac atacggtatt aagcaaggaa aaaactataa
     6181 gagaagtgct tagagaggct tttaagcata tgtttgactt agagcaagaa atgattgcta
     6241 tgtatgacaa aatgggagaa gctagtgatg atgaaatgag taagcttctt gaagaaacag
     6301 cagagataca aactatactt gaaaatagtg gtttttatat gatagatgca aagatacaag
     6361 aagttgcaaa tggtcttggt cttggagaaa ttggtcttga taaagatgta actgacttaa
     6421 gtggagggca aagaacaaaa gtacttctta ctaaacttct acttgaaaat ccaactattc
     6481 ttatattgga tgagcctaca aactatttag acgaagagca tataacatgg cttactaagt
     6541 accttcaaga gtatgagaat agttttgtct tagtttctca tgatattgaa tttataaata
     6601 atacatgtaa tgtaatatat catatggaaa atggagagtt aaatagatat aaaggaaatt
     6661 atgatgaatt tgtaagactt aatgatataa agaaacgtca agaagaacaa gcttatgata
     6721 aacaagttga agaaagaaaa agactagagg attttgtagc tagaaataaa gctagagtag
     6781 caactagagg aatggctaac agtagacaaa agcaacttga taagatggaa atattagaaa
     6841 gaccaaaaga aaaaataaaa cctacttttg cttttaaaga tgctagagca gctagtaaaa
     6901 taatttttga aacagagaat ttagttttag ggtatgatga agcattgaca aaaccactta
     6961 atttccattt agaaagagga aaaaagattg ccttaaaagg aatgaatggt attgggaaat
     7021 ctactctatt aaaaacttta cttggaataa taaaaccatt tgaaggaaat gttaaattag
     7081 gtgactattt ggaagttgga tattttgaac aagagagttc aagggaaaat agtaatacac
     7141 ctatggatga agtttggagt gaatttccag ggcttacaaa ctttgaagtt agacaggctc
     7201 ttgcaaaatg tggacttaca aatgaacata taacgagtca aatgagagta ttaagtggtg
     7261 gagaagctgc taaggttaga ctttgcaaag taatgttaaa aaatataaat ttcttagtat
     7321 tagatgagcc tacaaatcat ttagatgttg aggcaaaaga tgaattgaag aaggctatca
     7381 aagagtttaa gggaacagtt cttttagtat gccatgagcc agaattttat tcagaaattg
     7441 ttgatgatgt atggaatata gaagatttta ccactaaaat cgtgtagtga tttaacttat
     7501 tgctgggggg atttcatgaa aaagtttcta aatgtacttt tttcagtttg tattagtatt
     7561 gttataatag taggtgttat taattttact gttggattta aacaacttta ttattttgac
     7621 attgattact taaatatatc agagttatct ggtttatctg aagatgatat aaaattgaac
     7681 tacgattatc taattgatta taacttaaat aagaatgtgt ctgagttcaa gttaccaaca
     7741 ttaaaatctt ctccacaagg gaagatacat tttgaagaag tcaggaatat atttcaaaat
     7801 ataaataaat tagctaaatt attattggta gtttttttag taggaattat attgagtgta
     7861 aaaaataaaa atataaaaat cttgaaaact acatcaataa cacttataat tatgccatta
     7921 ttattaacag ttccaatttt attaaatttt gaaaaatcat ttattatttt tcataaatta
     7981 ttatttagaa atgattattg gatatttaat ccaaatttag acccagttat aaatatattg
     8041 ccagaggaat ttttttttca ctcaggaatg atgattttaa tattaatttt gttagcaagt
     8101 atcttactat ttgtaatgta tagattatat aaatctttca aaataaaata agatagcaaa
     8161 aacgaattat attaaaggtt atacaataaa aatagctatg taataatatt taaactaaga
     8221 gtagtcgata aatatttatg catagctatt tctaatagaa attacttttt actagtgatt
     8281 ttaactaaat cttgaaattt gcccataatg taattgtaat tatctttttt gtgaggtaac
     8341 atgcaactac tgcaatcttt gataccttta tctgtatact taaagtttcc accacagttt
     8401 tctcctaaag catacaaagg acaatagcaa aatagacagt taaactcttc tggtttatta
     8461 gttttatgac aagggaaaaa ttcgcaatct ttgtgattaa aaaacttata attttcgccc
     8521 atattttcct cctaattaaa taaaatttaa attttttata ctatataaga ttttggtgat
     8581 aatataatta tactaaataa ttttatgaat agtcgataat atagtattat taatcatagt
     8641 attaatggta taattagtca acagaaaaat aatgtaaaaa ttatgtaagt gtattttgta
     8701 attagaatat gttttataac aaaaaatgag gtgattaagt gaaagaaatt caagaaaaag
     8761 cacttcttgt aggtctgaat ttgactacaa tagtgaaaaa aaatgatgat atagacacta
     8821 gtgaatctat ggaagaatta aaagagctaa caaaagctgc tggggcagaa gttgtaggta
     8881 gcttaattca aaataaacat tctgtagatg cagcttatta tataggaaaa ggtaaagtag
     8941 aagaaataag agcctatagt gactctttag atgctacttt agtaattttt aatgatgaat
     9001 tatctggtgc tcaaatcaga aatatagaga atgttgttgg aagaaaagta atagatagaa
     9061 ctactttaat tttagatata tttgctcaaa gagcacttag taaagaaggt aaattacaag
     9121 ttgaattagc acaattaaaa tatagattgc cacgacttta tggaatgggt ggtgagatga
     9181 gtagaacagg tgcagggatt ggtactagag gtcctggaga acaaaaacta gaaatagata
     9241 agagacatat acttaataaa gcagcagaca taaggcgtga acttaaagaa gtaaaaaaga
     9301 atagggagac acagagagtt aagcgtttaa agtcaaatat accgattgta gcacttgttg
     9361 ggtatacaaa tgcaggaaaa tctactcttt tgaacgaatt aataaagaca cacaaggatt
     9421 atgaacaaga aaaagaagtt tttgtaaaag atatgctttt tgcaactttg gatgtaactc
     9481 taagaaaggc tcttttgccg aataaaaaag aatttttagt agtagatact gttggatttg
     9541 taagtaaact tccccatgat ttagtagagg cgtttaaagc aacattggaa gaagtacagt
     9601 atgcagattt aattttacat gtaattgatg cgacaaatac tagttatgaa ttgcaaaaaa
     9661 gtacgactga aggtgtatta aaagaacttg gtgtaaatga taaaaaacat atacttgtat
     9721 ataataaagt ggataagcta gaattggata tatatcctaa aagtcaagaa gatattgtgt
     9781 atatttctgc aaaacaaggc ataaatatgg ataagctatt aaatatgata gaaattgctt
     9841 taatggaaaa tacttattca gtaagcttga tgttacctta tgagagaggt gatatattta
     9901 gtagaataaa agataaatat aatgtggaga attttgagta tggggaaaat ggcataacgc
     9961 tagatgtaaa cttcgatgaa gaagatttta atatctatag agagtacata ctagaaaaat
    10021 aggttgaata tggatataaa ttgggaaaat atagataatc ttgaagatta ttttataacc
    10081 tatcttcttt ataaagaatc taaaacagta tctcaaatta gtaaaattag aaatatttca
    10141 agtactgaag caggtgagca acttatacaa gcaaaattaa aaataaagga aatgcaaaaa
    10201 gatgattttg aggcatctaa ggatatttta gataaatttt tagaactaga taaaattaag
    10261 agacttgatt ttatggatag cttagatgac gaaaaaatgg tatactttaa gagaaaggtt
    10321 ttcaaaagaa tccttgtgga aaaaaatgct gaagatttaa ttgttttaat ttgggcgaca
    10381 ggtgagctaa aagatgatag attcttaaag ttgcttcatc agctcacaaa ccacagacac
    10441 tcagatatta gaagaataac ttattcagca atcagaaaga ttgaatcacc aagtagtaga
    10501 gaagtactgc aaaaaggtct ctacgataaa aatgcacaaa ctagacagta ttgtgcaaag
    10561 gctctttcaa aactagggga tgaaaacagt ttaaagattt tacagcagtt gaaagacaag
    10621 aataagaatt ttgaaaaaga atatgtgctt agagcttatg acgaagccat taagtcattg
    10681 gaaaatgcaa aaaatttgaa aaagtaattt tttattaaag caatttgttt acttaatgat
    10741 gtatttctag agtttaacaa ggtaataaat aaattaatgg gaggggtttc aaatgatagt
    10801 aagacgttgt gcaggtggag tagtgtttta tgctaataaa gtacttattg taaaaaatga
    10861 taggggagaa tggacacttc caaaaggaaa gatacttgga ggaggacttc catatgagag
    10921 tgctgttcaa agagttaaag tagaaactgg catagatgct aaaatgatag atgtggctgg
    10981 cgatactatg tatgaatttt tctcaagaag tagacaacaa gaagtttgta atgcaataat
    11041 gtggtatgtt atggaagctt gtaacactaa ttgtgtactt gctcctgaat ttcaagaagg
    11101 tggattttat aaagtaaagg atgctttaga tatgttatca catcataaag agcaagcttt
    11161 agtagaagta tcttataaaa agtttaagga attaaagaaa ttagtttcag agggcgatga
    11221 tgttccagca aattaattgt gaatttagag gattctgagg aagggatagc cccttccttt
    11281 tttaattgaa gcagttttaa gcgtaaatta tatagtctct ttaaattatg atttatcaat
    11341 ttagagcttt agtagtttat atttaaaatc agtaagatat atttaaaata aaaattaaaa
    11401 agtatacaat tttattatat aattgtctta tgatattgat taaagaagta ttaaaagtcc
    11461 atttattttt aaatagaata attttataga tatataattt gatgtgtata ttagaaaatg
    11521 attaaaatag gaggaaatac aatgagcaat tacaggacat tacatgaatt tggaacagat
    11581 gaaataacta ttgaaaagtc tgtttttatt ggatatgcta agcctataaa gagtgaagaa
    11641 gaagcattag agtttataaa tgaaataaag aaaaagcata aagatgcaac tcacaatgtg
    11701 tgggcttata ctgtaggtaa aaatatgaat atacaaagat atagtgatga tggagaacca
    11761 caaggaacag ctggaatacc tacattagag gttataaaaa aagaagattt gagagatgta
    11821 gtggttgttg ttactaggta ttttggagga acaaaattag gtgcaggcgg tctagttaga
    11881 gcttatacaa aaggtgctaa attgggactt gaagcaggta agataattta taaggtaatg
    11941 tatcaagaag ttaaggttaa gatagactat actcaacttg gaaaagtgca aaatgagtta
    12001 atgaatttgg ggtattttat aaaagatact gtatatgagg ataatgttga gatagtagtg
    12061 tattcaagat tagaagatgt agagaaatta tcagaaaaaa tgattgatat aacaaatgga
    12121 acaggaaaaa taattttagg agaggaattt tatttgtctg aacaaaatgg agaaatttta
    12181 atataatcta aaaggttatt agaatagtta atacctcaaa tagccaatta gtatttttaa
    12241 gtgaaaagcc attaaattga tttaatctaa atcaatttaa tggcttttta tcggattttg
    12301 cacagaatcc tttttttact tcaaataaac cgactaaaag taaaaatatc ccaaatattt
    12361 tttttaatat atctgaagac agataattag ctaatagtga accaaataca gctcctaaaa
    12421 ccccaaacac agctataggt ataaccaatt taaaaattac atttttattt tttatatgta
    12481 tagcaagtgc tataagtgtc atagggatag atgaaagtaa atttacgctt tgtgctattt
    12541 tagtgtctat gccagcaagt aaaacgagtg caggtataag gattgttcct ccacccattc
    12601 ccattcctcc aataattcct gcaaaaaatc caattagagc aaataacatt aaaacaccat
    12661 ccttaatgct gcaattatca taattattcc aaaagaaatt ctaagaaact ttccagtaac
    12721 cttatttagt aatttagctc ctataatacc accaagaata cttccaactg caactttaat
    12781 tgttaagtta acatcataag tacctttaga tacatataaa acagaacttg ctgtcgttaa
    12841 gaatattatt atagaaagag cagttgcatg agatttatgt tcttctacct taacgatgtc
    12901 atttaatatt ggaactaaaa gagtacctcc tccagaacca aaaactccat ttataaatcc
    12961 tgtaaaaact ccaattatcg tattttttaa gttaaaatca aagtattttt tctttaaatc
    13021 atcagttttt ttcataaaaa tcaccttctg aaatagtttt accaaaatag gcaaattata
    13081 tttatataaa ataagattaa atataatctt tcatgtttgt ttaattttgc ataaaaaaaa
    13141 acatattaaa acaacatatt aatatatggt ataatatatt aaaacaaagt agcaaatttg
    13201 agaggagata acaatgtcaa atataaaaat acaaatagac aaaactgtag aatggcttat
    13261 aaataaggtt aatgaggcaa atgcaaaagg attaattgtt ggtgtatctg gaggaattga
    13321 ttcggcagta gttgctaatt taattaaaaa ggcatttcca gaaaactcta tgggagttat
    13381 aatgagcata aaaagtaatc ctcaagatag agaagatgca cttaaggtta tagagggatg
    13441 tgatatagaa taccttgatt tagaccttat agaaccacaa agtgctatat tagacatggt
    13501 agtaggaaat ttaaaagata aacatttata tagagcagaa tatttaaaga tgacagatgc
    13561 gaacttaaga gcaagagtaa gaatgagtac aatttataca attgctaaca atctaggata
    13621 tttagttgtt ggtacagaca atgctgcaga aatacacaca ggatatttta ctaaatttgg
    13681 tgatggtgga gttgatattt tacctattgc aaatttaact aaaggagaag tatatgaatg
    13741 ggcaaaagag cttggtgttc atgaagattt aataaacaaa gctccttcag caggtctttg
    13801 ggaaggtcaa acagatgaag atgaaatggg aactacttac aatatgatag atgctgtgtt
    13861 agaaggtaga ttggatgaag ttcctaaaag agaccaagag ataatagaaa gacttcatag
    13921 attaagtgaa cacaagagaa aaacgcctgc acaacctcct aagttctaat ttactattta
    13981 taagatttgt gataaaattt acaagtgatg aaaattagaa taattaattg tacgtattaa
    14041 ggagtggaaa tatgggacgt ataggtaata taattaatag aaaaggtaaa caagatgctc
    14101 aaagagcaaa gatatttact aaacatgcta gagcaatagc agttgcagct aaagaaggtg
    14161 gagcagaccc agagtacaat gcagcgttaa aaacagctat agaaaaagct aaggcagata
    14221 atatgccaaa tgataatata gatagagcta tcgctaaagg tgctggagca ggcgctggag
    14281 aagactatga aactatagtt tatgaaggat atggacctgg tggagttgct gttatagtag
    14341 aaactttaac tgacaacaaa aacagaactg ctggtaacgt aagatactat tttgacaaaa
    14401 acggaggaaa cttaggaact agtggatgtg tgtcttttat gtttgataaa aaaggacaaa
    14461 tattagttgg tttaggagat ggagtttcag aagaagaatt gatggatgtt gcattagaag
    14521 caggagctga agatttcata acagaagaag atggttatga aataataact acaccagaag
    14581 atttttctag tgttcgtgat gagttaaaag ctaaaggata tgagtttata tctgctgatg
    14641 tgaagatgat acctcaaact acgactgtat tgactgaaga atctcattta aaaatgatga
    14701 ataaattagt tgatatgctt gaagaagatg atgatgttca agatatatat cataactggg
    14761 aagtagaata aatagtataa gcatattgaa atttataagc attgaatcaa ttttaacatg
    14821 tgataaattt tatagaataa gaaattacta aaatagacat acgcacctct aacctatatt
    14881 tgcataataa tatgtaagta taggtaaggg gtgtttttat atttataggt taagaaattt
    14941 aaagtaaatt ttgagctgga cttgattgtc tataaataat tgatgggtat ataacttgtt
    15001 gctgaatacc atttatacgc ccagttactg tatcaataat catcctaaga ctacgttctg
    15061 ctgtctcttc catttttaaa tctataactg tcaaaggagg aatacttctt gccattagtc
    15121 gtgaaggtcc attacttgct gcaaatattt gaatatcctc tccaattaca attccagatt
    15181 catgaaatgc attcatcata ccaagagcta caacatcatg attacaaaaa attacttttt
    15241 ttagtgaatt ttttgcaagc attttttgag caatatcata tccactatct attttattag
    15301 aacaataata tatagaatct gatagatgta ttccatgttc tttacatgtt tttaaaaaag
    15361 atttacccct taaagtcata catgtaaagg aagttgggtt gaatacaaca gcaatatcat
    15421 ttttaccatt ttgaatagca tgtaaagctg ctatacgacc agtttcctca ttatcaatag
    15481 atacacttga aaaaccaggt atttcacgat ttacaacaac tacaggaatt ttaggtttat
    15541 tttttactag atattctata tcatcagcat tgatacctgt taaaacaatt ccatcataat
    15601 aattatttgc aaaaaagtat tcatcatcac atagatgacc aactttgtaa ggtcgtatga
    15661 tgatatttat gtttaaatgt tctaaagaaa ttatgtggtt tattgcttga agtatattgg
    15721 ttgatgccaa ctcaaatccc tcatcctgcc aataaaaagc tattttaata ttattatgtt
    15781 taataggagt agagcgttta tatccaagct tatcagctga atttaatata agctcttgtg
    15841 tatgtagcgg aatcctgcgt tcatttcctt taccacttaa gacgtacgag acagttgctg
    15901 aagaaagacc acagtctttt gcaatatctt tgattgttgc catgataaaa tcctccttaa
    15961 atagttgctt aaatttgctt ataccaataa tcttaacata tattcaacta aagaacaata
    16021 ttctacaagg tgcatatttt acaggattag aataaataaa tttaaaaaaa cttcttactt
    16081 atgttgacat aatttagttt ataggattat aatatgctta aataagctta ttattaactc
    16141 aaattaagca aagttaagca ggaacatgca atgctagctt gcaatgtgac tgtaaagggg
    16201 gaaaaggtat gagtaaggta aatatatttg aagttggtcc tagagatgga tttcaaaacc
    16261 tgaaagagtt tttagaaact gataaaaagc ttaagattat tgatggtctt attgcttcag
    16321 ggattaaaca acttgaaatt agttcatttg taagcccaaa agcaatacct cagatgaaag
    16381 atgcaaaaga agtagcaact tattgtgtag aaaaatatcc tgatgtgaaa ctttatgcat
    16441 tagttccaaa tttatatggt gctaaagttg catgggagtg tggtataaga gatatatcct
    16501 atgttatatc tttgagtgaa actcataata aaaaaaatat aaatcgtacc catgaagagt
    16561 ctattgatga gcttaaaaaa ataatgaata cttatccaga catgaatata tgtattggaa
    16621 tggctactgt atttgggtgt ccttttgaag gtttaccaaa aattgaagat gccataagat
    16681 ttgctaaacg cctttgggaa aacggtatca gggagataaa tctagcagat acaataggga
    16741 ttgctgaccc taagcaagta agagaaacta ttagtgcatt agttgaagct ttaccagagt
    16801 gcagatggca ggtacatatt catgatacta gaaatatggg aatggttaat actcttgtgg
    16861 ctattgaatc tggaattact actgttcagt ctacacttgg aggattagga ggatgcccat
    16921 ttgctcctgg agcatctggc aatactgcaa ctgaagattt agtatatatg cttgaacgta
    16981 tggggtatga tacaggagtt gattttgtta aacttcttga attatcaaag tatcagaaga
    17041 gttttgtaaa tggagtatat agtggtcatc atgtaaatat aaagtctgat agttgtaatt
    17101 gttagttcta ttgattaatc gattaaaaaa atatgttgat taaaaaaata tgctgattgg
    17161 taaaatagat ttattgataa aaaagataaa ttataaaata cattatctaa taaaacaaat
    17221 aaaaaaattg agggggatta gtatggatat tatagcttta cttttatttt taggagttat
    17281 tattattgca tttgtacgta agaataatgc aggtattttg gctctagcta ttggtgtcat
    17341 agctgttaga atttttggaa tggatgataa agaactttat ggagctataa gcaactcaat
    17401 gtttactaca cttgttggta taacactact ctttgcaata gtgaactcaa caggagcatt
    17461 aaagcttgtt gcagaaaaaa ttgtagctgc ttctggtaaa cgtgtttggc ttatacctat
    17521 agcagttttt atagcaggat ttgttgttgc tggtgttgga cctggggcaa ttccagcact
    17581 tgcaattatc ccagcaatgg ctgttcctgt agccataacg gttggttata atcctgtaat
    17641 gcttgcactt ataggtgaat gtggattaat ggcaggtcgt atgactccta ttactccaga
    17701 aggtcaaatt ataacaaccg ttgcagaagg ggttggaatt gaaaatgtta tgcctactat
    17761 cttagcctgt caaactcttt ctacaattgt attttcaata gtattgttta taattttcaa
    17821 aggatataaa cttaaaaaac ctataaatgt tatagatagt aagcaaatag aaaaatttaa
    17881 tataaagcag ataatttctc taataagcat agtagttatg attgctttta tcataatttt
    17941 tgatatgaat gttgggcttg cagcttttgc agtttcagct gtacttattt ttttcaatat
    18001 tgctgatgaa ggagaatgta tcaaatcaat tccatggagc acgatagtaa tggttcttgg
    18061 agtaggtgca atgatgacaa tcgtagatgc agcaggaggt attgacttga tgagtaatgc
    18121 attatcatca ctgatgaata gtaaaacagc tactccaatt atgagtattt cagcaggact
    18181 tatgtctatg gttagttctg ctttagcagt agtttatcca actatgatgc ctatgtgtgt
    18241 tgatatagct aaggcagttg gaggagtaga cccactagca cttattgctg cagtcggtgt
    18301 tggaggttct cttgctggtg taagcccact ttctactggt ggggcattga ttttagcagc
    18361 tatgggttca agtaagaaag actttaataa ggaagaacaa aataaggttt ttgtacagct
    18421 tttcattatg agtgcagttg gcttattggt tattgcagta gtatcaatat tatcatttaa
    18481 tgcaataaca catttaatga actaattata ataactgaaa ataattcatt aaatttaatt
    18541 tttaatatta atcaatataa tataatttgg aggggaagtt atgaaaaaag gagcattaag
    18601 caatataaag gtacttgacc ttacaagagt tcttgcaggt ccatattgta caatgatgtt
    18661 ggcggatttt ggagctgatg ttataaaaat agagatgcca agaaaaggtg atgatacaag
    18721 aggttttgga ccttttgtaa atggagagag tttatactat gcaaatataa atagaaataa
    18781 aaaaggtatc actctaaacc ttaaatcaga agaaggaaaa aatatatttt tagaaatggt
    18841 aaaacatgct gatgttgtag tagaaaatta cagaccaggt gtaatggaaa agttaggtct
    18901 gggttatgat gagttggcta aagttaacaa tcaaataatt tatggagctg tatcaggatt
    18961 tggatgttat ggaccatatt cagaacgtcc aggttatgat attattgcac aggcaatggg
    19021 tggtcttatg agtataacag ggcaagaagg aggagaacca acgagagttg gtaatgctat
    19081 gggtgatgta ctaggtggaa tgaatttaac tattggtata ctcacagcac tcaacgctag
    19141 aaaaataata ggaaaaggac aaagagtaga tgtttccctc gttgattctg ttgtatcgtc
    19201 tctcgaaact ggtatacaga gatactttga ttcaggacaa cctgaactta tgggtaatag
    19261 atatgcttca gcctatcctt atgactcttt taaggcaagt gatggaagtt ttgtaatagg
    19321 ctgtggtaat gacaaacttt ttgaaaagtt atgtaaaaaa gttcttttta gagaagacct
    19381 tttaatagat gagagattta aaacaaatct taagcgttgt gaacattaca aggctcttaa
    19441 accagaaata gaaaaatggt ctgttaagca tacaataaaa gaagcagttg acttaatatc
    19501 agaggctgga gttcctgctg ctcctatcta taatcttgat tgtgttacaa cagatgaaca
    19561 tatagcaaaa gcaagagaaa tgattgtaga tattagtcat cctattatag gaaatatgaa
    19621 agtcaatgga aatccagtta agttgatgac aacaaaagct aaaattgata tgccagcacc
    19681 atctctaggg gaaagtaacg aggagattta taatggttgg cttggttttg atagtgataa
    19741 aataaaagat ttaaaagaaa atggagttat ttgattaaaa aatagctaaa cacacttgtg
    19801 actttagtca tgggaggttc agttagaata atatcaaaaa atattaaatc aatactgtaa
    19861 atcaaatata gaattgtcag aagcatttaa gatgggaaaa gctacttaga cattgagtct
    19921 ttgtggcttt tcctattttc attaagaact ttatattatc agaatagata tgtactaaac
    19981 taaagtatta acaatttaaa ttaatagaaa ataaactacc taattatgtt gaataaagac
    20041 tactttagtg aaaataaact tatagagatg ctatttaaat tattttaatg gaaatatcaa
    20101 aaatgagact gcattctcaa aaatgagact gcgtttttta agtattttct cgaaaatgag
    20161 acaaaaatat tgttttatga gttttattga agaaaaattc ttctgtgaat tacaatttaa
    20221 tcaattttaa agttgaaaat tattaaaaat ttagtgaaat ttatgtatat ttcgtttatt
    20281 ttattaaaaa taacaatttt ttactaaaaa taaagaactt caatggtgat tggcatagta
    20341 cttgctattt tatattaaca agaaatgtag aaaaaataac aatgtataat ggggggaata
    20401 tgatggaatc tgtaatggaa aatttgaaca atcttaaggt ggaggttaag gacaaagtct
    20461 gtgttattac tataaataga cctaaagctt taaatgctct taatagtgac actcttaggg
    20521 aactatcaca agttattgat gtagtatcag aaaatgaggc aatattagga gttattatta
    20581 ctggagaagg taaagttttt gtagccggtg ctgatatacg ccagatgcaa aactataaaa
    20641 gtgaggaagg cagaaaatat gcaggttatg ctcagggaat ttttgataaa attgaagctc
    20701 ttgagaaaac agtaatagca gcagttaatg gatatgcatt aggtggtgga tgtgaacttg
    20761 ctatgagttg tgatattaga attgcatcag aaaaggctat atttggacaa cctgaagtta
    20821 atttaggagt tattccatgt tttggtggaa ctcaaagatt atcaagactt gttggaactg
    20881 gaatagctaa agagttaatt tttacgggga gacaagtaaa tgcagaagaa gctaaatcta
    20941 ttgggcttat taataaagtg gttccaagtg atttactttt agaagagtct atgaagatga
    21001 tgaaccaaat agttgaaaaa gcgccaatag caataagata tgcaaaagtt gttattaaca
    21061 aagggataga tatggattta aagaatgctc ttgaactaga gaaagatatt gcaggtttaa
    21121 cttttgctac aagagataag caagaaggaa tgaatgcatt tatagaaaag agaaagccag
    21181 tttttgaaaa taagtagcta ttataactaa attggaacaa tgtagaatat attcaaaatc
    21241 taaatttcgt gaaaaaatac atatagttta attttaaaag gagttaattt atgaatacgg
    21301 gagtaattat aagtttagta gggattatca tagctctatc tcttctagtt gtaatggtta
    21361 tgaagggaat aaatattttt attatagcaa ttgtatgttc atcattagtt gctataacag
    21421 gaagcctaaa tgtgtatgat gcacttaaag tggattatat gacaggattt gttggatttc
    21481 ttcaggcaaa tttctttatt ttcctaacag ggacattaat gggaaaaata atggagatta
    21541 ctggtggagc aaaatctatt gctaagatga ttgtaagatg gattggtaag gataaagcac
    21601 tactttctat tccaatagct tgtggaatat tagcctatgg tggagtaagt gttttcgttg
    21661 taagttttgc agtgtttccg atagctttag aaatttttaa agaagctgat ttgccacgaa
    21721 gatttatacc agcagctttg acttttggat gctcaacttt tgctatggtt gcaccagggg
    21781 caccacaaat tcagaatatt gttccagcat caacattagg gacagatatt atggccggag
    21841 cagtaaatgg ttttatatca tgtgcagtaa tgtttatagt tggaagtacc attttgtaca
    21901 aaatggtttc aaaagaaaaa gccaatggtg gtcattttat agcacatgaa tcagatatat
    21961 ttgatgaagc agaagcaact aattctaaga gagaaaatgg tcctaatggt gtaattgcct
    22021 taattccatt gatagtgtca attttaataa ttaatgtaaa agttaatgga aaagcgatag
    22081 taccaattga ggtgggagta tttattggag ctttattagt atatttattg ttaaataaat
    22141 atcaagataa tactaaaata gttggacatg ttggtgatgc atgtaaaact acagtagtag
    22201 caatatgtaa tacttgtgct gtagttgcat ttggttcagt agtaaaatca gcagttggtt
    22261 ttgacttcat agttaatgcc atgactaata ttcctggtcc tcctattgca ggggctgcag
    22321 ttggaactac agttattgca ggtatttgtg ggtcagcttc aggtggacta ggaattgcag
    22381 ctccacttct tggaccagta tttttagcca agggtgtttc cgctgcagca ttaactagga
    22441 ctatggcaat ttcatcagca gcattagatt cacttccaca caatggatat attgttactg
    22501 taacaaatgg tctgtgtaaa gaaacacata aggatgccta tatgccaata ttctgggtaa
    22561 ctgttttaac tccgtttatt ggaaccatag tagcagtcct actgtttaca atgttcccgg
    22621 gattgccata agagaaaaga taaaaggtat aaattatatt ttgatattat aaaatcaatt
    22681 ttcaaaatat atacaataaa tttcatgttt taatactata aatatgtatt taaaataaca
    22741 aattgaccat ataaggagga attgaaagtg ttaaataaac cattagaggg agttaaggtt
    22801 attgacctaa cgtattttgt agcaggtcca gggacttcta aaatattagc tgattggggt
    22861 gctgacgtta ttaaggtaga gccaagcttt ggagacccag gaagaaagac aggggcaaca
    22921 atgacaatgc caatagatga ttataataat cctttttaca gcacatataa ttcaaataaa
    22981 agaggtttgt ctataaatct taagagtgaa actggaattg aaattatgga taagctttta
    23041 agtgaagcaa acgtatttgt ttcaagttat agaacaggag cattaaaaag actaggtctt
    23101 gattatgaat cattaagtaa aaagcatcca caccttatct ggggtcagat aaatggtttt
    23161 ggcgattttg gacctgctaa ggataatgca ggatttgata cagttgcctt ttgggcaaga
    23221 agtggggcta tgcttgatat agcagaaaaa gatacaagtc caataaatcc acctatagcc
    23281 tttggagatg caacaacttc ttgtagttta tctggaggaa tatgtgcagc tttgtaccag
    23341 caagctaaga ctggaaaagg acaaaaagta atggcatctt tatttgggca agcaatatgg
    23401 aatgcatcat ctttattagc atctacacag tttagtgatg agtatccaaa aactagaaaa
    23461 aatgctattt caccagtaat aaattcttat cgttgtaagg atggaaagtg gatattctta
    23521 agtattttag agcatgaaag atattttgag gcactttgta gattatttgg aagagaagac
    23581 ttaataaaag aagagaaatt tgcatcttca gtagaaagta agaaaaatgc tacagaactt
    23641 attaatattt tggatggtga atttgcaaag tttactcaaa atgagatggt tgaaaaactt
    23701 actgctgctg atatagctca tgaaaaaatt caacatgtta atgatgtagt gactgatgaa
    23761 caggctattg ctaataacta tatatatgag catacaagta aaaatggaaa taagacaatg
    23821 cttgcatcca ctccagttaa atttggaaat attgaagtta acatgacttg tgatgctcca
    23881 cttataggag aacattcaga tgagatattg agagaacttg gatacagtga ttctgatata
    23941 gaagctctaa ttgaatctgg aattgtaaca atgaataaag aagcatgtga aatatgttaa
    24001 taaacatgtg aagtatgtta ataaatagga gggaatttta tggattttag attaacagaa
    24061 gcacaattaa tgcttcaaag agtagcaaaa gaatttgcag agaatgaaat agcaccaata
    24121 gctgctgaaa cagataaaac aggtattttt ccaagggagc tttttagcaa aatggctaaa
    24181 ataggattta atggcattgg aacacctgta gagtatagtg gttctggagg gacagatata
    24241 gaaaaggtaa ttgttgttac tgagattgcc aaaaaatgtg cagcatctgc agcaatatta
    24301 tcaatacaca caatatatgc tcaagctatt ttgaaatttg gtactgagga acaaaagaaa
    24361 aaataccttc caatgatggc tgaaggtggg tgtgtaggtg ctttcgcctt aactgaacct
    24421 aatgctggtt cagatgctgc aagagctgca acaactgcaa ttattgatga agaaacggat
    24481 gaatatgttt taaatggaac taagtgtttt atttctggtg gaggtcaagc agaatcatta
    24541 ataatatttg cactgacaga cccatcaaag ggaataaagg gaatgtctgc tatcattgtc
    24601 gacaaaggta ctccaggttt ttcaatagga aaaattgaag aaaaaatggg tattcatggt
    24661 tcagaaactg cagagttaat atttgataac tgtagagttc caaagtctaa cttattagga
    24721 aaagaaggca aaggatttaa tatagctatg acttgtttag atggagcaag aattggaatt
    24781 ggagctcaag ctgtaggaat tgctgaaggg gctttagaag aaagtatcaa atattctaaa
    24841 gaaagagtcc agtttggcaa gccaatttca gcgttgcaag gtattcaatg gtatatagca
    24901 gatatggcga caatggtgga atcagcaaaa ttattagtat attatgctgc agatttgaaa
    24961 gctagaggag aaaaacacac taaagaagct gctatggcaa aatacaatgc gtcaagtact
    25021 gcaagagagg ttacaaatct agcccttcaa atccatggtg gatatggata tatgaaggat
    25081 tatccattag agagaatgta cagagatgcc aaaataacag aaatttacga aggtacttca
    25141 gaaatacaca aagttgttat ttcaagagca gtactaggat aggaggaaaa gaatgaatat
    25201 agttgtttgc ttaaagcaag ttccagatac taatgaagtt aaaataaata aagagacagg
    25261 tactctaata agagatggtg taccaagtat aatcaatcca gatgatagaa atgctttaga
    25321 agaagctcta aaaatgaaag atgaactagg agcagtgatt aaggttataa gcatgggacc
    25381 tcctcaagct aaaagtgctt taaaagaagc tttggcaatg ggagctgatg aagcttattt
    25441 aatctcagat agagcttttg gtggttcaga tacttgggct acatctacaa ttattgctgc
    25501 tgctatagaa aaggttggaa agtatgatgt aatattctgc ggaagacaag ctatagatgg
    25561 agatacagct caggttggac cagaggttgc agaattttta gggataccac aagttactta
    25621 tgcaaaagaa gtaaaggttc aagatgacaa attactagta actcgttata cagagacagg
    25681 ggattattta atagaagcaa agatgccagt tttattaact gctataaaag agttaaacaa
    25741 tccaagatat ccaagtgtaa aaggaatctt agaagcttat aataatggag atgcgaagat
    25801 tactgtatta acacttgcag atttagatgt tgatacaact caaattggat taaagggttc
    25861 acctacaaat gtttataagt cttttgcacc agttaaagac aagcataatg aaataataga
    25921 aggtataaat aaaaaagaaa aagctgagaa actaatagaa atactattcg atttgaaact
    25981 agtataggag tgttaagatg atgagagcaa aagttaatca aggtataaat ttaaatgatt
    26041 ataatggtgt ttgggtaata ggggagcaaa gggaaggcaa aattaatcct gttacgatag
    26101 aacttattgg tgaaggaaga aagcttgctg accaattagg aaaagagctt gaagttgtga
    26161 tagctggata tgaagtagag aaagaagtga aggaattact tcactatagt gttgataaag
    26221 tatattacat caatgaccct cttcttaaag atttcactac tgatggatat gcaatttcaa
    26281 ttgcaaattt aatcgaaaga aaaaagccag aggttgtatt ggttggtgct acttctatag
    26341 gaagagatat agcgcctaga attgcaggta aggttggaac aggtctaact gctgactgta
    26401 caaaacttga aatagattca acagataata agttattaca aacaagacca gcatttggtg
    26461 gaaatttaat ggccactatt gtttgtccaa aaaatagacc acagatgtca acagttcgtc
    26521 caggagttat ggcaaaagct gtaagaaacg aatcagagac tggaatttta gaagttgtta
    26581 ctccagaact tactgaaaaa atgattagaa ctagattagt agaggtactt ccacaagaaa
    26641 agaaatctgt aaatctaact gatgctagaa taatagtatc aggaggaaga ggattaaaaa
    26701 gagcagaagg atttgagctt ataaaagaat tggctgataa attgggggca gagattggtg
    26761 cttcaagagc agcagtagat tcagggtgga tagaacattc tcatcaagtg ggacaaacag
    26821 gaactactgt tagaccagaa ctatacattg catgtggaat atctggagca atacaacact
    26881 tagcaggtat gagtgattct aaatatatag ttgctataaa taaagatgca aaagccccta
    26941 tttttagtat atgtgactat ggaatagtag gagatttata tgaaattata cctgaaatga
    27001 tagaatcatt aaataggtaa cttttagaaa acattgaata tgtagaatga taagtcttat
    27061 aatattgtat tgtaagactt attatttttg ctgaaggtta tagtggggag atgaaacaat
    27121 gaaagagcag tggtataagg atatatttgc aagaatatta tctatgactg atgatggttt
    27181 tattgttgta aatacaagtg gagtaataat agatattaat gataaatatt gtgacttttt
    27241 aggaaaagaa agaaaagata taataggaca aaatatacaa agtattatac caaatacaaa
    27301 aatgctagat gttatgaaag ataagtactg tgaagaagga gctatacatc attattcagg
    27361 tggaaataca aaagaaaaaa gtgtaatagt aagtaggtct tatgtagaaa atgacaatgg
    27421 agaagtagtt gcaggagtag ctcaagttaa atttagattg caatcatttg atgtagctaa
    27481 aaaattaatg tcagaatata tggagctcca atattataaa gagcagttta aggataactg
    27541 cggttttgac aagctcatag gtgaaaatag agacttcata gaacttaaga aaacaggtgt
    27601 gaaagcttct aaaacaaact ttccagtact tttaacgggt gaaacaggca caggtaaaga
    27661 agtctttgct agagcaattc acaataatag cagtaggtca gataaaccta tggttagtat
    27721 aaactgcgct gcaataccag aagaattatt agagtcagag ctatttggtt atgacgaagg
    27781 agcttttaca ggagcaaaaa agggtggtaa aaaaggtaag tttttagttg caaataatgg
    27841 tacaatcttt ttagacgaga taggtgacat gcctttgacc atgcaagcaa aacttttaag
    27901 ggttcttcag gaaagtgaga tagaaccagt gggaggtctt aaaactataa agatagatgt
    27961 aagagtaatc tctgccacaa gaaagaattt aagtaaaatg gttgaagagg gattatttag
    28021 agaagaccta tactatagat taaatgtaat aaacattcat atgatggaat tgaaggacag
    28081 gcaagatgat attttattgc tagcaaatta tatattaaac aaattaaatg ttgaatacaa
    28141 ggaattgaag gttttaagtg ataaggttaa aaattgtttt attaactata cttggccggg
    28201 aaatataaga gaactacaaa atgtaataaa aagtgcctat gctgtaagtg atgatatggt
    28261 cattatgatg tgtgaccttc catctaagat ggataatatc agcagagtag ctcaatgtaa
    28321 tgtggatagc aattgttcaa ttcatgaaat ggttgagaat tatgaaaaaa gcttaattat
    28381 tgatgtgtta aggaagtata attggaaatg ttctaaggca gcagaggtta tgggaataca
    28441 caagagtctt ttatataaga aaataaaaaa gtatgaaatt gaattaaata attaagataa
    28501 cttgaattaa ggcaatgcta aaaaaagcgc ccttattcaa agcgaactgt ataaactgtt
    28561 ttatgcagtt aaaacgtgta aaagtagatg tgaagtacta atgtcaatca ttagtacttt
    28621 ttattgtatt tataattact atctatattt cagtatcttt tgatatctaa gattattatg
    28681 tttaatttag gatataacaa aaccctctta gttattgaca gttttttata ttttaactgt
    28741 ctactaagag gggatagttt agtttaaatt tagagtaaat tttatctagc atttataaaa
    28801 aatattttga atagcttttt tgttatttag tcataatttt ctacttcttt atcaagttct
    28861 ttgccacaag aatcacactt atatatagca ttgtatccat aatcatattc atcaactatt
    28921 ttcttttgtc taaatacacc accacactca cataggaaac caatttttat aagtgtaacg
    28981 ataaattgtg attttataaa ttttatatca ttacatttat cgttagtgta aaatgtataa
    29041 atataagagt tatcgcactc atctcttaat tcaaatcttt caatatatgt gttatttttt
    29101 aattcctcaa attcttcttt agttaaatat gatttttgtt gcaattcatg taaacgctta
    29161 atttggtcca ttatttatat cctcccaata attttagtat tttaagctta gataaggaat
    29221 ctaagtttta ttaaatttat cccaaggaat tatttaaatc atatttagtt ttattaatta
    29281 caacatatat aaattacacg aaaataaaac taattgtaaa aataatatcc tgtgaaaacc
    29341 taaactacta accccttact aacccgaaaa ctaaaaaaag aaaatatatc caaatataag
    29401 tgatatataa ataaagatgt tttatataaa tttaacttta aatattctga caataatata
    29461 tataaatata ttatagttaa aatataccac atattttggg cattgtatac tggaattaga
    29521 gtaattaatt tattttttaa catacaaatg attttatgaa aaaaaacaag actcaaaaat
    29581 ttttatattc tttgatagaa taaaattgag agtaaattta ttgaatttta taaaatttat
    29641 aaggggtgag tctaagatat gcatgaagat aaaaatgata tatttgaatc aatagatact
    29701 gaggattttg ataataaaac agaagaagaa tgtttagagc tagtaagaaa aaatggattg
    29761 aatttaatat acataaaaaa tcaaacagaa gagatatgtc tagaggctgt tagacaaaat
    29821 tgtaatgcaa taaaatgtgt tcagaatcag acagaaaaaa tatgtataga agcagttaaa
    29881 caagattgga gaatgttaga atttgtggaa gagtcaacag aagaaatttg catgttggct
    29941 attaaccaag atggaacagc attaaaatat atagaaaatc agacagagga gttatgctta
    30001 agagccgttg aaaaaaatgg tgcggctttg gagtatgtaa aagaacaaac agaagaaata
    30061 tgtatagaag cagttagaaa cagcgaattt ggattggcaa gagtgaaaaa tcaaacagaa
    30121 aaaatatgta tggaagcagt taagcattgt agctataatc taaaatgggt agaaaatcaa
    30181 acagaagaaa tatgtatgga agcagtgaga caaaatgggc ttgatttgaa atttgtaaaa
    30241 aatcaaacag aaacaatctg tttaagagca gtaagacaaa atggaatggc attagaattt
    30301 gtaaaggaac agactgttgg tatatgctta aaggcagtaa gacaatatgg aatggcatta
    30361 aaatttgtaa aagaacaaac agaagaaatt tgtacagaag ctataaaaca agataaaaga
    30421 gcacttagtt ttgtaaaagg agataaggaa aaatacaagg ctctatatga taataatgaa
    30481 ccatttgcaa agagatatgt tagaaatgta atagaaaaag aaaacagagc attgattaaa
    30541 aaaggagaag aaaatgcaaa aatcaaaatt gcaggaaaac tttatgatag aggcatgtct
    30601 tttgaagata tatcagatat agttgagatt gaaataagca agttaaaagt gagtttaggt
    30661 gttttttaaa gtaataaatg taggtgtttt ttatgggaaa tagttaataa aaaaataatc
    30721 gactagctct ttgtaggaca gcaaaattag tgatataatt tgatgtataa taaattattt
    30781 ttattagata agggggaatt aaagttgaag attacagtat tataccaaac aagaagtgga
    30841 aacacagaaa gggtcgctaa gctgatagaa gatggagcta aaaaggtgga tggagtagat
    30901 gtaaagctta tgagacttga taatattgat ttagattatt taaatgaaag taaagcagta
    30961 atatttggaa cacctactta tttagcaaat acaacttggg aagtaaaaaa atggtttgat
    31021 gaagattcaa aaaaagttaa tttagctgga aaattaggag ctgtttttgc tacatgtgat
    31081 tatatatgtg gaggtccaga tgttgcgata cttactatag taggacattt gatggttaaa
    31141 gggatgcttg tgtattctgg aggtggttct ttaggaaagc catttataca tttaggacat
    31201 gtacattcaa ttgaaggtcc agagttacaa gatgagaagg ctataatatt tggtgaaaga
    31261 attgctacta aggcaaaaga actatttgct taatttctat atgcataata atgattagag
    31321 taaatttagg gttttaatgg agatttgaag taaaagttat aaaataaaaa attttccata
    31381 aaattactca agatgaatta taatataatt ataaataaat tatattatta aaatatttaa
    31441 aatcaaacgt caatagcgag ggaagtttat ggtatggcat atagaatatg agattttttc
    31501 tgcaattata gttatttttt taatggtata cttttttaga agtaaattta tacctacttt
    31561 acaaaacaaa atatattgtg cacttttaat attctcattt ttatttatac tttcaaatat
    31621 tttaggttca ttttgtctaa ataatataga caaaattcca atttttatta cttttttatt
    31681 gaatcagata tacttattgt tattgccaat accagcagct cttgtatcat tttatgttat
    31741 ggctattatt tatcaagata taaggtatat gaaaaaaaga ttattatttt tatctatacc
    31801 acttataatt tgtataatct tatctattac aaatcctttt acgcatattc tatttggatt
    31861 gtcaaaagaa acaggttata tacggggttg tggatatgtt gcaacttttg catctttttt
    31921 cttttatgca atatattcag catttttatc ttttaaatat agaaaagcaa tgcatgaatc
    31981 aaaaatatgg gctattagag gttttcttat agtttcaatt gtggcaatta tcttacaggc
    32041 aattttttta cagtatcttc ttacaggaac tgcatgtgca tgtagtttac ttttgacata
    32101 tctttctatg cagaatcgag gtcttattat tgatgacctt actggtgtac tcaatagaca
    32161 atcttttatt caagagctag atttgaatat caatactgaa caatcaggat ttattattac
    32221 aattgcgctt gatgatttta agtttatgaa tgaaactttt ggaacaaaaa atggagatat
    32281 tgcactaaaa gaagttggaa aatatttgat tcagatacat gatgtaaacc atgtatatag
    32341 aataggggga gatatatttt ctattgttgt caatagtcaa ttaggtatag aaccagatga
    32401 tattatcaga aagatagaag aaagatttaa aaagccttgg ctagtagaag agataagttt
    32461 taatttagca actagtattg ctgttgttta ttacccagaa aatgcagaaa cgacagaaga
    32521 tgtaattgtt gctatagatt tctctataca tgaagcaaaa cgttctgagt caaggcgagt
    32581 tgtttatgca gatgcgtcta taagtgaaaa gataaagaag aaacatgtta ttaaaaattg
    32641 tttaaaagaa gcattaaaaa atgatgggtt tgaagtgtac taccaaccta tattttctaa
    32701 tagcaggggc aggtttactt cagcagaggc attacttagg ctagaacaca aagagttagg
    32761 ttttatacct cctgatgaat ttattccaat tgcagaacaa acaggattga ttaactcaat
    32821 tggattagta gtctttgaga aggtatgtag atttatagct agtgatgagt ttgaaaattt
    32881 aaaattggat aatatagctg tgaatctttc tgtagtacaa tgtatgcaaa aaaacttagc
    32941 tgatgattta ctttatctta tgaataaata taatgtatca ccaactaaat ttaaacttga
    33001 aattactgag actgtggcta tgggctcttt taatataatt aaagaaacaa tggaaagatt
    33061 aattgattta ggtgttaagt ttgcacttga tgattttgga attgcttatt ctggagttac
    33121 taatatgctt tctttaccat tttcagtaat taagttagat aagagtttga tttggtctat
    33181 gaatgaagat agtaggcata agcttacagt tgagacgatt atagctctta taaataaatt
    33241 gaatatgaag gctgtagcag aaggtgttga aaccattaag tgtgctgaag atttaatagc
    33301 tatggagtgt gaatatttgc aagggtatta tttttctaaa ccagttccag aagatgtttt
    33361 taaaaatttg ttgaaagaaa atacttttac aagttaacac cctttatttt tttaattaca
    33421 actttgttca ttgacactgc cagcgatata ttataaaatt aacacaataa taaatattaa
    33481 atttattata catattatta atattaatta atggagtaaa gatttattat attttattta
    33541 attatcctaa agtgagaaag gaagatgtga catgaataaa aagaaaatat tagtgccaat
    33601 tgatgggaca gaaagaagta tgcattcttt ggaatttata aaaggaatat ttaaaaaaga
    33661 tgaagttgaa gtagaaataa tgaatgtaaa agaactagta tttattgatg gcatatccct
    33721 ggctgaagag ataaaaaatt ctgaaaattt aggaagaaga atcttggata aagcagcaga
    33781 gataatggga gagtatgatg ttaaagtaca ttttactttt ggatatccag gagatgagat
    33841 aattagaaaa gcaaaagaag ataatgtaga ttttatagtt atgacaaaat ctactaaaaa
    33901 aggtcttaca agaatgatag gttcagtaac agcaagtgta gttaaacaag caaaatgtat
    33961 agtgatgatt gtaccggagt agaattagaa aggtatttga aatatgaata aaaagatagt
    34021 agcagtttgt gaaagtgaaa gaagtttaaa ttctcttaag acagctgcaa atgatttagg
    34081 atttgattta gtttatgaaa ttcaagaaaa tgataatata aaaaatgaac tttctattta
    34141 tgatatagaa gatgcatcta tagtgttatt tgttacaagt gattctatag aaagcataga
    34201 gaagattgat agatttatag atagagaatt ttatgaagtt gatccaaagt atataataga
    34261 ggatgcaaaa tctatagtaa gtgaaatttt aatagactta aattaatcgt gggtgtcgca
    34321 ctaagaatag agcgacgcct ttttacatat tttacataag aaataaaggg ctgtctcaaa
    34381 ctatagataa gactcttttt cattgttgaa atgatgaaaa tatatttgat ttcatacaaa
    34441 aaagagtgcc tcataactaa aaagttcact ttgagacact ccatttttat atgttagttg
    34501 catttaaggt aaaatagaag tttaatcatg ttgaaataac tgaattacat cttctaaatg
    34561 attgtacatt gatgtataac ctaagtaaga gtctttacta tcaaatgcca tcattatctt
    34621 gttatggtgg ctaatcatat ccttaagaga atttctatat ttaaaaccga agtattggtt
    34681 agaagaaact gttttaggta ttaactcata atattttata aaaattggat tatgagaagc
    34741 taaaattatc tctttgtgaa attgtaagtc agcatcataa cattcttcta tcaaaccatg
    34801 agaaacctta tccaattcct ctattgactt taggtaagta tattttattt tttgaagttc
    34861 tttagatgta gcatttaaaa ttgctagttt agtagcttca ctttcgataa gttgtcttgc
    34921 tcttaaagat tctacataaa attcttgtat gttatgattt ttatagattt caccatcatc
    34981 aaataggtca acttttttaa atccttcaac tataggactt gagtaaggca cagctctaag
    35041 tattccttcc ttacagagta tatttatggc atttcttaca ggtattctac ttatgttaaa
    35101 taggtcagca atctttctct cacttggtaa tttactatta attttaaaag ttccatttag
    35161 aatgtttaat ttaatataat caataatttt ttcagtggca tttaagcttt tcataaaaac
    35221 tcctaaacat ataatttttt tatgattgta ttatatcata gttaagagtt aaatctactt
    35281 ttttataata tgtataagac aggatagagg ccaaaatagc tataatagcc aagataaagt
    35341 atccatattg tattgaagta tattccgata ttttaccaac tatcattgga aatacagaca
    35401 ttcctatagc ataaattgct tgaaaaaaac ccattgcagt agatttctga tgttttggta
    35461 catttttcat agactcgcta gtaaggtaag aaaggagtat acctgtagat atacctggta
    35521 gcacttgtag tacaaatatt atattaatat tttttattgt tggaactaaa aagcaatagg
    35581 ctgaaactaa aataaaaact aaaggaatcc aaaattttgg cccatactta gtacaaaatt
    35641 tagtagaagc aaagccagaa gaagcaacag cacacaccat gtaagatata gaagatagac
    35701 ctatgagggt agaactagaa cctaagtttt taagaatttg agtagtaaat gacattgttg
    35761 tagacatttg aataccctgt tgaattaatg ccataaagga aaaaaataca agtcttttat
    35821 ctttgcagat agataaatat tttttaattg atattttttc aggtattttt tttgaattat
    35881 gattttttat atttaaggct agaatcgttc caataactga agacaataca ctcatgatac
    35941 ataaggctgt cattcctaat ttttcataaa ataaagtact cataacaaat ccaatcaaca
    36001 ttcctagatt gtttaccaat attatcgtac tcgtagcttt ttgttgatgt tctgtagaaa
    36061 aaaagttggt atagtgaacc atgtaagaaa tccaagttga tgatgatatt ccagataaaa
    36121 tgttagctat aagaaaacca ttgcctccag gagaaaagac tcttataaga gatgctaaac
    36181 cagcaaagaa aattccagac agtataaata ttttatgttt atttatagaa tcagcaaata
    36241 ctccaacagg aatccttaat aaacattgtg agattccata tgcacctatt attataccaa
    36301 tcatatttgt agatacatta catgcactta aatatggatt ttggaaaggt atatatacat
    36361 attgtgaaaa ccagaaaaat gtaacaatag aaagtaaaag taatttttga ttagatattt
    36421 ttttcatatt atcctccata tggtattttt ataatgcaaa taatatacca tttagatttt
    36481 ttaatcaagt actttctcta ttaaaaaaaa tttaaatata taattattat ttacaaaaaa
    36541 attatacttt atagttgaat taagaaaacg cttatgatac tataaagtta acaaatttga
    36601 atatataaca gtagacgtgt tactggtaat gcaggcaaga tcgaatcaat ttttatagat
    36661 tatctataga gtactatggg tataatctat ttatttggtt cgattttttt gtttaattgg
    36721 ccataaaaca aaaagcctat agttgaaaat ggaggatttt ttatggatta taaaaatata
    36781 gctcaagaaa ttctactaaa tgtaggagga aaagagaatg taaatgaagt gactcattgt
    36841 atgacaagac ttagatttaa agtcaaaagc gcttctaaag tgaataagga taaattatct
    36901 aaaactgaag gagtaataac tgttgttgag agtatgggtc aaatacaagt tgtaattgga
    36961 aataaagtaa aaacagtata tgatgaggtt ataaaaattg tacctcaatc aaatgacgta
    37021 ggtaaaaaga atgaaagtca agaaaagcaa agtataatta attctatatt atcagcagta
    37081 gctggtatat ttacacctac aatacctgct atagcagggg ttggtatgat taaaggtatt
    37141 ttatcagttc ttgctatgta ttatatgaat aaaaatggag tagatataaa agaaacgcaa
    37201 agttatatta tcttaaatgc catggccgat tctatattct attttatgcc tattatacta
    37261 ggatatactg cggctaaagt atttaatgca aataaaataa tatctatggt attaggagct
    37321 acactttgtt atccaacttt tactgctttg atggcaggtg aagaatctgt tagattttta
    37381 ggattggcag taacaaaagc tacgtataca tcatctgtta ttccaataat tattgctata
    37441 tgggctcttt catacgttga aaaagtatta gaaaagtata tacctgaggt aattaaaata
    37501 ataatggttc caactttatc attggttgta atgttacctg caacattatt tttatttgga
    37561 ccaatcggaa tttatatagg aaatgtcata aacttttctt ataagtatat ttatgaatta
    37621 agcccagcgt tgtgtggagc atttgtaggt gggttatggt gtgtgttggt tatatttggt
    37681 gctcatagag cattattacc aataggtata agtgatgttg ctcaaacagg aagacagaat
    37741 ttattggcat ttgcaggagc tgcaaacttt tcacaagcag gggctgctct aggagtattc
    37801 tttaaaacta aaaatcaagg actaaaaaca atttctatgt ctgcaactat aacagcttta
    37861 tttggtataa ctgaacctgc aatatacggt gccaatctta gattaaaaaa acctatggta
    37921 tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg gaatgggtgg agcttatggt
    37981 aatgcatttg caaaccaagg tgttttaact atacctgtat atgctgaagc aggtgcatta
    38041 ggtttcttaa gttatctagg tggttgtgct atagcatttt ttggttcagc tatttcaact
    38101 tatctagttg gatttgaaga tttggaagag tcttctaata aggaaaattc atttataaaa
    38161 gtagaaacga aggatggtgt tattgatata acttcacctg tagagggcga atgtatagaa
    38221 ttgtcagaag taaaggatga tgtatttgct tcaaaagcta tgggagaagg tatagctgtg
    38281 ttacctacaa aaggagtaat aacagcccct actgattgtg aagtagcttc tttatttcca
    38341 acattacatg caataggatt aaaattagat aatggagcag aaatgttaat acatgttgga
    38401 atcaatacag tagagttaaa tggtaaatac tttacaaaac atgttaatca aggagattta
    38461 gtgaaaaaag gtgataaact tatatcgttt gatatagata aaataaaaaa ggctggttat
    38521 gatgtaacta cgccagttat agtaaacaat acatttgatt ttgggcaagt agtttcttgt
    38581 aaatctagtt atgtttcaac caatgataat ataatttcat tagtattagc tggtaattaa
    38641 gttaatttga ctagagataa gtaaattgat ttaaaaggaa ataagtaaat caacaattaa
    38701 atacatctaa aagtaagttt attgcaatga attgttaaag aaaataatta atatggagta
    38761 aagctattgc aatgaagtaa caaagaaaat aattaatata gagtaagttt attgtaatga
    38821 attgttaaag aaaataatta atatagggta aatctatctc aatgaaataa caaagaaaag
    38881 agttaatata gagtgaactt attgcaatga atcaacaaaa aaaataatca atataaaaag
    38941 gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca tcatcttctg
    39001 catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta gccgactata
    39061 attcttttaa aaagtctcat ttacaggcag atactaaagt tgcatcagac ttttaccata
    39121 attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat cgtttttcaa
    39181 tttcatgggc aaggataata ccagatggtg aaggagagat aaaccagaaa ggtttagact
    39241 tttataataa gataatagac aaactaatag agtgtgatat tgagccattc gtaactttat
    39301 accattttga ccttccattt aaattagtag agaaatataa tggttgggaa agtagagaaa
    39361 ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga gatagggtaa
    39421 agtattggca acctcataat gaacagaatt taattgttag agtggaggag agaattaata
    39481 tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg gattataatc
    39541 tttgcttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa gaaagtaaaa
    39601 ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca gaagatgtat
    39661 atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg catcattatg
    39721 gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatacagtt cctcacatgg
    39781 aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct gtaaattatt
    39841 ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt ggattaagag
    39901 aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat cctaacttag
    39961 aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc ttaaatgaat
    40021 attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga gctaaagata
    40081 tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga agtcatataa
    40141 atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt ccatggtcat
    40201 ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt gtttatataa
    40261 atagaactga ccatgaagtt ttagatttaa aaagaatcaa gaaagatagt ttttactggt
    40321 ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat attgagggtt
    40381 ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag gatgataatg
    40441 atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc gggtctgagt
    40501 taaataaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata aaaaagagta
    40561 ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa atgataattg
    40621 catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg gccttaactg
    40681 accacattgc ctttgcagtg agacgcttta aagaaggtat aatgattgaa aatcactatt
    40741 tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat gctattactg
    40801 tttttaagga ggttcttggt ttagacctac cagaagaaga gattggtaat atagcaggcc
    40861 attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga agtgctaaaa
    40921 ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat aataaagagt
    40981 cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga tttctggcta
    41041 atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa aattgtaagg
    41101 aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac tccagagaat
    41161 taccaagaca agaggagtta tatttgatga ttcatattca taagatttta ggagaattat
    41221 gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta aaaattaact
    41281 acactttaaa tatgaacaat tgcattactt aagggggttg tcttaaaatt gattttttta
    41341 attaaatttg aagctcaact ccttttttaa acctttatat cattacaact ttaatgttta
    41401 tacgcctgta ctttagatgt atatctactt tttacatttc tcaacatgca aacctaccat
    41461 tttttgcagt atgttaattg aaataaacca ttgagttaac cctaaattta ttctggactt
    41521 ataggaatag ttccaaagat actttaaaat attttctgga ataattgctt atttcacata
    41581 tgttttattg tgatgttttg atttaataaa ttcttcaact ctgtatatag taaatgtatt
    41641 atcatgtttg ttttattcaa gcacacttat aatacgcttg attttttcac aatgatgaaa
    41701 tatactaatg ggtgttatag gtataagaat cgaattacac ttgaagaagt aagtgataaa
    41761 tgagatataa atcctcatat gataaactac tatttcattg aggaacatat tctaagtgat
    41821 gtcaagatac tacaatatga ctaaattcct agtgataaaa aagtctacga atgagtgtag
    41881 aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat ctttgtattt
    41941 tgttaaaaaa gagtatttca attgaacaga ttgaagcaaa ctattgccat tctgggagag
    42001 atggactggt tgaactttca aaacaagatt atcagttagt agttctagac gtgatgatgc
    42061 caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt attcccatct
    42121 taatgctgac atcaaaaagc gataattaca gcaaagtgca tggattgcga gctggtgcag
    42181 atgactatct aaccaaacca tttgaaatta cagaatttac tgctcgtgta ctgtctttaa
    42241 tccgacgtta cacccgtttt aatccaaaaa atgaggaacc acagcaactt tctttcaaag
    42301 gtttgttgat tgaccttgac cagcatacag tgactaccaa tggcgaacct atgttgcttc
    42361 cacccaagga atttggtgtg cttctttact gtgcaaggca tcagggtaag attttgacaa
    42421 aacagcagat ttatgaagct gtgtggggtg agccatatgt atatgatgac agcaatatca
    42481 tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct cccatctatt
    42541 tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa tggatgaaca
    42601 aacgataatt cttttcttat tcttgctgtc gattggtcta ctttttcttt ccatatttgc
    42661 tttttccaag cttgggcata ttcacaaacg cctaaaagat atagaggaga tactggctga
    42721 tgtacaaatt ggaaacgaaa atcgcaaaat actaattaag ccatgtgatg ttatggctcc
    42781 attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac tttctctgaa
    42841 aaagtcggat aaagctagca aacaacttat gacaagtcta tctcatgatg ttcgtacacc
    42901 attaacaact ctgattggtt atctggatgc tgtacacagt ggtatagtta tcggacaaga
    42961 acgggaggaa taccttgaaa tcgctaggcg tagagcctac gacctcaagg attacattga
    43021 tgttcttttt gactggtttc ggttgaactc tgatgaattt acgctgtcaa tagaatctgt
    43081 ggaaattgca gagttgtcac gcaatatttt gaaagactgg attcctatat tccatgaaaa
    43141 gaaactgaat tttgaaattg atattccaga aaatcgacta atggtaaatc tcgacccaga
    43201 tggatattcc cgtgtggtta ataatctggt tcaaaatgtt cttgctcaca gtaaggctcg
    43261 gcaaatcaag attaccatga gtgaggattc cagaatggta ttactccgtg ttgatgatga
    43321 tggtgttggg attgccagag aaaatttacc acatattttt gagcgactgt ataagtgtga
    43381 taaaggacgt tcagaaaaag gaagtggtct tggtctatct attgtcagtc aaatggtgga
    43441 gcgaatgggt ggtcagattt ccgtagaaag tgagattgga aagtataccg tatttacagt
    43501 gagcttacca ttaatataaa attgtttttc ccccatgtga catgtataca cagggggatt
    43561 ttttgtaagt tataaagttg caaggttaat acaaggttgt tgcaaggtta atgtaagatt
    43621 gaggtggtat gatagcttta ataaacaaag gaggatttta ttatttatga ataatttaat
    43681 tattgaaaca aaaaatctca ctaagcaata tggtgctcaa aagagcgttt ctaatttgaa
    43741 tattcacgtt cagaaaggtc gcatttacgg attgctagga agaaacggtg cgggtaagac
    43801 gacaactatg aaaatgttat tgggtctaac aaaaccaact actggagaaa ttaaaatatt
    43861 tggtcaggac attcacaata acgagaagaa gcttcttcct cgaattggat gtttgattga
    43921 atcaccaggg ttttatccta atctgacagg aacagaaaat ctcaaaatct ttgcaaggct
    43981 tagaggaata ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg gtttgccaaa
    44041 caatgacaaa aagttatttt ctcaatactc tcttggtatg aaacagaggt tggcgattgc
    44101 tcttgccatt atgcatgacc cacaactgtt gattttggac gagcctatca atggacttga
    44161 tcctattggt attgcagaag taagaaagtt tattcgtgat ctctgcacag aacgaggtaa
    44221 aactattttg atttccagtc acattctttc tgaaattgcc ttgttggcag atgacattgg
    44281 tattattgac catggtattt tgttggagga agaaagtctt gcccaattag aagaaaaaaa
    44341 cagtaaatac atacattttg ctgtatctga tgcagcacat gcagcaaggc ttctggaaac
    44401 ccaacttaac ctgaaaaatt tcaaagtagc agataattac aatattatgc tatatgatac
    44461 agatgtttct gttgcagaaa taaatcgtgc tttcattcta aacaacattg aggtatcaga
    44521 atctcattta tgcgaagata ctcttgaaga ttacttcaag aaagtaacag ggggtgaagg
    44581 aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa tttatactga
    44641 tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt gcaaaggaca
    44701 acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt ttgctattac
    44761 caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat aacgatgtat
    44821 taaaaaatct tgcaacagtg ccagtgccaa aaggaaaact ggctctggca aaattggctg
    44881 tgttgctgtt tatatcattg ttctactccg ttgcagggtt aggagctact atcattggag
    44941 gtttcattgt tggaatagta gagggggtta ctgtaaaatt aggaatgagc attgcttttg
    45001 gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat ttcaatcgca
    45061 gctatatttt ttctattata ctttcttttg tatatgctat tttcaatttt agtatatcct
    45121 taaatattat caattttgaa ccaaataacc cactattaag cgtattacct gctcctgtta
    45181 taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg gcacttagac
    45241 agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata accattatag
    45301 cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta tctatgcttg
    45361 atataattac tacagaattt caaaaaataa aaagatataa tattctatgg attggtgttg
    45421 ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca cacggcagtg
    45481 accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc agtcttagtt
    45541 ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat acagaccaaa
    45601 ccttaaaaaa catcctaact gttcctattt cattacgcaa attgctagtg ggaaaattaa
    45661 ttgcggttgg aggaattgct gtattgttaa gtcttttcag cttcttatgc acatttattt
    45721 tggggttgtt attttgcaat atcaatatat ctgttacact cattgtaaaa tccttagttc
    45781 agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt gcttattttg
    45841 gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac gggttttgtg
    45901 gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca ggtcttggtc
    45961 tagttggata caacaatggt gctggtactg tttatcagcc acttatcggt gctatgactt
    46021 tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat tatgataaag
    46081 ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat aaataacata
    46141 aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg
    46201 gctatatgct catgtattca agaagctaca gttttgattt ttcataactg tagcttcttt
    46261 ttgtatttat taaatcacgg ctatcataat acttgcaata ggtatagcga caagagagag
    46321 tactgtcatg tgtgttttat atattacatt aagtatattc taaaatatat gttttaaata
    46381 atatatttga aaataggaat atttaaataa aaaaaataat attatatata gttgacaaaa
    46441 attggcaaat gatataatat ataaatatag aaatagtaat cattactatt attaaaaagg
    46501 gaggaattaa ttatgaactt aaaaggaact aaaacagaaa agaatttaat ggcaggattt
    46561 gcaggagagt cagaagcaag aaataaatat acatactacg cttcaaaagc aagaaaagaa
    46621 ggttataatc aaatagctgc tatatttgaa gaaactgcta acaatgaaaa ggaacatgct
    46681 aaattatggt ttaaactttt acatgatggt atgccttcaa cagaggaaaa tttaaaggat
    46741 gcagcagcag gtgaaaacta tgaatggact gatatgtatg caaaatttgc taaagaagct
    46801 agagaagaag gatttgacaa gatagcttat ttatttgaag ctgttggaaa aatagagaaa
    46861 gagcatgaag aaagatactt aaagctttta gaaaacttaa atgaaggaaa aatatttaaa
    46921 agagatgaag aagttgtttg gcaatgtcaa aactgtggtc atgtgtatgt aggtactgaa
    46981 gcaccagaaa aatgcccagt atgtgaccat ccaaaagcgt atttcaatat aaaagctgaa
    47041 aattattaaa aattatcatt attaaaaagg aatgttaact atgaaatttt ctaaacaacg
    47101 agaactgatt ttaaatgaaa tattaaataa tccggttcat cctactgcgg attacctata
    47161 tgaaaactta aaaaaagata atccaaattt aagcttagga actgtttata gaaatttagc
    47221 tcagctaacg gaacatggct ttataagaaa ggttagtatt ccagggtatc cagatagatt
    47281 tgatggtcga atagataatc actatcatat catatgtgaa gtatgtggag aggtatacga
    47341 tttagaatcc gaagttctta ataatttaca agaattaata tctgaggaga cagacataaa
    47401 aataacatct tataacataa gttttaaagg gatttgtaat aattgtaaaa ggtgtagcca
    47461 agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt gagtttatgt gtagtgaaca
    47521 aaaatttttt atatgtgaga cttgtggaaa ccttgtagga atgatacaaa gtggtggtgt
    47581 acctattttc tgttgtggaa aacctatgaa ggaacttgtt cctaatacaa cagatgcagc
    47641 agttgaaaaa catgtacctg taattgaagt ggatggaaat aatgttactg ttaaagtaag
    47701 tagcacaact catccaatga ctaaagaaca tcatatagca tgggtgtatc ttatgactga
    47761 acaaggtagt caacgcaaat gtctagcagt agatggagaa cctgtagtta aatttgcttt
    47821 gaatgatgat gataaagtga tttctgccta tgcttactgt aatttacatg gattatggaa
    47881 ggctgaactg taatatttaa ataaattgag tcaaagagga ggctataaat gtattttcat
    47941 acaaatagcc tcctataaat ttatataaat tcaagtttta attttgttta atgtcaatat
    48001 aatataagga gaaaataata tgtcaattta taaatgtagt gtttgtggat atatttatga
    48061 tgaatctaag aatgataaaa catgggatga attaagtgaa gattgggaat gccctgtatg
    48121 tacaaaaggt cgtagttatt ttggaaagat aagcactgtt tactatgaag aagatgaaaa
    48181 gatagcagaa gacatagttg aaaatgaatc taaattaaat actgaaaaag agggagattt
    48241 aaattactta agtacatatt taagaagaga tgatgaagtt gagaaacata tggatataat
    48301 tcacgaaatg gctgttacag gaaaatctat aatagaacct atgagaacta aactaccagt
    48361 aatttcttgg gatgatattt taattatggg tgctcaatta aatccattac ctttaaatga
    48421 gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa gcaaagaaac ctatgattat
    48481 agaaaatcca gtttatattt cccacatgtc atttggagct ttgtctaaag aactaaaaat
    48541 agctttagca aagggtgcag cccaaaataa gacagctatg tgtagtggtg aaggaggtat
    48601 attaccagaa gagaaggaag cttcatacaa gtatattttt gagtatgtac caaataaata
    48661 tagtgtaact gaagaaaatc ttaaaaattc tgatgctata gagataaaaa ttggtcaagg
    48721 aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa gtaactgaag aaatagcaaa
    48781 agtaagaaat aagcctgtag gtcaagatgt aataagtcca tcttgttttg aggaaataca
    48841 atccaaagag gatttaaaga aacttataga tgaacttaga gaagtttcag aagggcgtcc
    48901 aataggtgtt aaaatttcag caggtcatat tgaaaaagat atggaattta tagcttatgc
    48961 taaacctgat tttgtaacta ttgatggtcg tggtggagct actggagcaa gtccaaagtt
    49021 gcttaaagat gcaacttcaa ttccaacaat atttgctctt tatcgtgcta gaaaatacat
    49081 agatacacat ggattagata tagatttggt tataacaggt gggcttcgta tatctactga
    49141 ttttgctaag gcaattgcta tgggggctga tgcagttgct attgcaagtt ctgctcttat
    49201 ggcagcggcg tgtcaacaat atcgtatatg tggttctggt aagtgtcctg taggagtagc
    49261 tactcaggat gaggaattga gaaaaagatt acatatagaa aatagtgcta atagagtggc
    49321 aaacttttta aatgtaagtt tagaagaact taagactttt gctagaattt ctggtcataa
    49381 agatatacat gatttaagtg ttgatgattt atatactgtt aactctgaaa tatcaaatta
    49441 tacaaacata cagcatgtat agattgaagt ttgaaactat tagattttag atagctataa
    49501 aagtaaatga taaatatatt ttataaaaga gagtgtctta aaatgaactt tttagttcat
    49561 aagtcactct tttttgtatg aagccaaata tatttatcat tttagcaatg aaaaagaatc
    49621 ttatctcttt attttgagat agactccttc tatttatgaa aattaaagat attaaattgt
    49681 aaaatattct gtgatgtcgt actattaaac taaagattct gtactttaaa gtaaataaaa
    49741 atttaaggag tgcttattat ggaaagattg aagatacaag ttttagttga caataatact
    49801 tatattgata gatactttgt tggagaacca gcagtaagct attatattga gattgatgga
    49861 aataggattt tgtttgattc gggatattca gatgtattca tatcaaacgc tgaaaaatta
    49921 aatatagatt taggaaattt aactcatgtt gttttttctc atggacataa tgaccataca
    49981 agaggtatcc agtttttaga aaataggtat gatttatcta cagttgaact tatttcacat
    50041 cctaactgtt ttatacctag aaaacatgga gagaaaagca taggagctcc attttctgct
    50101 gaggaaatta aaaatatttt tatgtataat cctaaggata agccatttaa tctaagtaag
    50161 aattgtgtgt ttttaggtga aataccttct ataaataatt ttgaaaaaag agctaaaatt
    50221 ggtaaatgta aaataggaga tttgtgggaa gatgattatg ttttagatga ttctgctata
    50281 gtttgtaaaa ctgataaagg tatttttatt gttacaggat gttctcatag tggaatttgt
    50341 aatatagtag agtatgctaa aagggtatgt ggagatgata gagttattgg tatactaggt
    50401 ggttttcatc tttttgaatt gaataatagg ttagatagta caatacaata tttggataaa
    50461 gaagaaataa gaatgttgta cccttgtcac tgtgtttctt ttaaggtaaa agctaagatg
    50521 aatgaaatat tgaatataaa tgaagttgga gtagggctga ccataagtat atagtatttg
    50581 aaagtatata agatttaagt tgaaggttaa aaagatattt tctatataaa gtatcttttt
    50641 tgttacgttt attttaagag aaatcaatat acttttatat gaaatattgt aaaaaaattt
    50701 atttttaatg agaatatatt ttcattaata aacaatttta ttatgtatta ataatgctag
    50761 aataaatata aataaatcaa agttacttac attaaagtat attttttcac aaaatatatt
    50821 gatttattct tttaaagttt gataattcca atgaatgtaa taaaagaagg gattttatac
    50881 tatacaatta ttattataaa attataaaga aaaaattaaa taataataaa gaaaaaatgt
    50941 ataatattta acattaaaaa agatatttca taaaattaaa aaacaaagtg aatttgaata
    51001 ctatttaaaa aataaaaaaa tatataaaaa atatttaata caaaggtaga aataagttga
    51061 aaatgtaaag tcattggaaa aatatagttg aatttaatga acaaaaatgt tattgtttta
    51121 tcaatgaagt gttaaaataa aaactttcaa tatcatttta gggaggatga tttcatgcaa
    51181 aaaagtgtaa agaaagcaaa agtaacggga tctatgttag ccatttttgg tgttgcaagc
    51241 gttcttttta gttcacatgc aggaggagga tttgctacag ggaatcaaga aactcagtac
    51301 tatgtgcagt atggatggac agcaccactt atggcaattt tagcaatgat tatacttact
    51361 gctaccatga gagaagttat cataatgtat aataataaca attgtaggaa ttataaggat
    51421 ttattttgtg agctttggag accatatcca aaacttgaaa taatatggga gatatattac
    51481 tatctaatgg ttttaatagc tgtaagtgct gttatagcag gtgcagcagc agtatttcaa
    51541 agtataggag taaattattt tgtagctgta tttattattg gagttgtact tcttgtcttt
    51601 actatatttg gtgctatgct tgtatctaaa gcagctacag caatgacaat agctattcta
    51661 gtatgtactc ttactatttt tattgttggt ataaaagcta aagtaccaga aataactgaa
    51721 atattatcaa atagaacgag tttcacacct ggctatatca agccaatact taatactttt
    51781 atttatgcag gttttcaatc ggttgttata cctacacttg caggatgttc aagaccactt
    51841 cttaagaact caaaagaagc tactaaggca atgataattt catttgttat gaatgcaatt
    51901 gcattaggac ttgctgtaac tatgctaatg ggatggtata gagaaattat tgctgctggg
    51961 caaacaacac tccctactct ttatgttgca ggtcaatctg gaaatcatac tatatacata
    52021 atttataatg ttgcattatt cctttgttta atgtctacag gagtaactac tatatttggt
    52081 cttgtaaata gatttgaaga ccataaagca ctttctttcc tttcttcaag aatgaaaaga
    52141 agggtattta cagcttgtgc aataatggta gtgtcaatgc ttatatcact tacaggatta
    52201 agtaatattg ttaaatatgg atatggatat tgtggttatc ttggtctatt cactatagta
    52261 attccattcc ttacactagg acattataag aataagaagt ttgcaaagga aaatccagaa
    52321 gctaaatggc cagcggaatt aaatgaaaat gtaaattaga tgtagtagca aaaaatattg
    52381 ctaaagagaa tactataaat aagaatacta tagataatat atctgcatgt ttattaagat
    52441 tatttgcaaa atctaatatt gaacaactat aaacaagcaa tataaaacta agaataaaag
    52501 ataaaaagag ggtgtctcaa aatggacttt tagtttataa ggcactcttt tttatatgaa
    52561 gtcaaatata ttttcattat tttgacaata aaagagacta atcctacttt ggatagcctc
    52621 ttttttcttt aatataaaat agtataacag caaagtatag attgatacat gttttctgta
    52681 gctttctata tattacagtg tttgtgtggc attggtattt taagcaatct ataaattata
    52741 attctataat tcttctacta attttagatt ctatagccgt ctctatttca cttaacattc
    52801 gttcattttt agggtcaata aaagcacatg tatagccttc ttcaccagct ctaccagttc
    52861 taccaatacg gtggatgtag tcttctggtg tttctggtaa atcgtaatta tatatatgac
    52921 ttactccaga tatatcaagg ccacgagaag ctacatctgt agctattaag tattgtatat
    52981 ctaaatttct aaatgatttc attattcttt ctcgttttga ttgagcaata tcactatgaa
    53041 gtttttgaca attatatcct cttatagcaa gagcttcttc aagattgtca acccttctct
    53101 tagtcctaca aaatattatt gccataaaag gattatcttc atctagtacc ttacataagg
    53161 catctaattt ttttctatct gtagtttcta ctacttcttg ttttatatta ttaagagtga
    53221 tttcttcttt ttgaatagaa actacaacag gattttttgt gtatctataa gctaattttt
    53281 ttacttgaga atttatagtt gcagaaaagc atagtgtttg ctttttgttt gaagtctctt
    53341 ttaaaatagc ttccacttcg tttttaaatc ccataagcaa catttggtct gcttcatcta
    53401 aaacaaaagt ctttaactta ctaaggtcta ttgtttttct atttagatgg tctaaaagcc
    53461 tacctggtgt agctataatc atatgaatat ttccttttag tttctttatt tgagaaccaa
    53521 tatcttttcc tccatatgct gcaagaatac ttacatcttt agattcttta agtttcatag
    53581 cttcttctgt tatttgaata gctagttctc ttgtaggaga aagtataagt acttgtatat
    53641 catttatatc taatgatata ttttcaaata tcggaagtaa aaaagctagt gtttttcctg
    53701 ttccagtttg agcttctgct attacatctt ttccttcttt tattagttta atactttctt
    53761 tttgtatagg tgtaggtgtt gttattcctg atttttttag aatatttatt gtattttcac
    53821 taatccctaa gcttttgaaa tccattgtta tcttaccttt catgaaaaat tttctttttt
    53881 attaaccaat taataatatc atatttgtaa gtatttttag taaatttttt aattattatt
    53941 tatatagctt aaaaatatat taagatactt taaaatggaa tgtaacttaa cttaaatttt
    54001 agtaggaatt ttataggttt atttttaaag gttttttgaa aataaatcta tttaaaaatt
    54061 aaattaaaat gcaagaaaaa cataataact ttcattttta agaagttttc acaaaaaaga
    54121 aaaaatatag tgcaataaat gatacaaagt aatataatag attacaagat atgaaaagct
    54181 aaaaaaataa attgcaaatg aaattaaaat gaataaaaaa taaaaaaata atcataagtt
    54241 agaggtatca atgaaaaata agacttaatg tttttaaaat taaaagtata ctaagggggg
    54301 cattaatatg tgcgttatat caaaagatag agcaaaggaa ataaaaatag tagatactac
    54361 tttaagagat ggagagcaaa cagctggggt tgtatttgca aatagagaaa aaataatgat
    54421 tgctgaaatg ttaagtgacc taggtgttga ccaaattgaa gttggtatac ctactatggg
    54481 tggagatgaa aaaaatgtaa taaagcacat atgtagtaga aatttaaaat cagatataat
    54541 ggcttggaat agagcagtaa taaaagatgt tgaagaatct atttcttgtg gtgttgatgc
    54601 agtagctata tcaatatcag tatctgacat tcatatagaa aataaattga gaacttctag
    54661 aggctgggtt ttagaaaata tggctaaaac agttgaattt gcaaagaaaa atggattata
    54721 tgtttcagta aatggagaag atgcttcaag agcagatata gacttcttaa ctgaatttat
    54781 aaatgttgga aaacaagcag gggcagatag atttagatac tgtgatacag taggagttat
    54841 gaatcctttt tctataaaga atgcaataga aactctttat gaaagaacta attttgatat
    54901 agaaatgcat acacataatg actttgggat ggcaactgca aatgcacttg caggaatagc
    54961 agcaggagca aattatgttg gtgttacagt aaatggatta ggtgaaagag caggaaatgc
    55021 agcattagaa gaagttttaa tggcacttaa gtgtgtttat aaatgtgatt taaataatat
    55081 agatactaga aaatttagag gaatatgtga gtatgtagca caggcatcag gaagaatact
    55141 tcctacatgg aaaccagtag ttggggataa tatgtttata catgaatctg gtatacatgc
    55201 agatggagct ttaaaagacc cgcataacta tgaaccattt gacccaagtg aagtaaatct
    55261 tgaaagaaaa atagtaatag gtaaacattc aggaagagct gcagttataa ataagttgag
    55321 tgaatatgaa atgtatattt ctccagaaaa cgctaccaag ctattaaatg ctataagagc
    55381 tacatctata agattaaaaa gaagtttaat ggataaagaa attcttcaat tatattgtga
    55441 tatactagca catgagaaag gcacaacaga agaagaagca gttagaggtt catatattta
    55501 agatataatt gtttaggagg aaaatatggg agataatata gtttataaaa taattaaaaa
    55561 acatatagta gatggagaag ctgttgctgg aagttcaatt ggtataaaaa tagaccaaac
    55621 tttaacacaa gattctactg gaacaatgac atatcttcaa ctagaagcta tgggaataga
    55681 taaagtaaag actaaaagaa gtgtggcatt tgtagaccat aatatgttac aacaaggatt
    55741 tgaaaatgca gacgaccata agtatataca aacagtggca gataaatatg gagtttattt
    55801 ctctaagcca ggaaatggta tttgtcatca agtattttta gaaagatttt caacaccagg
    55861 agatacactt ttaggttctg atagccacac acctactgct ggaggtgtgg gcatgatggc
    55921 tattggagct ggtggattag atgttgcttt agccatggca ggaggagcat actatataaa
    55981 agcaccaaaa gtctgtaagg tgaatctagt tggaaaatta aataatatgg tatcatcaaa
    56041 agatataata ttagaagtgt taagaaaaca aactgtaaaa ggtggagtag gtaaagttta
    56101 tgaatatggt ggagaaggtg tcaaaagcct atctgttcca caaagagcta caataacaaa
    56161 tatgggggct gaacttggag caactacttc aatcttccca agtgatgaaa aaacactaga
    56221 attttttaag agtcaaggaa gagaagacgc atggatagag ttaaaaccag atgcagatgc
    56281 agtttatgat gaagagatta ctataaactt ggatgaatta aaaccattag ctgcaaaacc
    56341 tcatagccca gacaatgtag atgaagttga aaatataggc aagattaaaa tagaccaagt
    56401 ggcaatagga agttgtacaa attcttctta tgaagactta atgaaagtag cacaaatcct
    56461 gaaaggaaat aaagtacata aggatgtgag tttggtaata gcaccaggtt caagacaagt
    56521 tatggagatg attgctagaa atggggcttt ggcagatata ataagtgctg gagctagaat
    56581 actagaaaat tcttgtggtc catgtattgg tatgggtcaa tctccaggta ctgactctgt
    56641 gtctttaaga actttcaata gaaatttcta tggtagaagt gggactttgt cagcacaagt
    56701 atatttagta agtccagaag tggcagcagt aagtgcaatc aagggagtgc ttacagaccc
    56761 aagagaattt gatattaaat ttacaaattt agatgtaaat gaatttttga tagatgatag
    56821 catgataatt aaacctgctg atgtaggaag taatgtagaa gtagttagag gacctaatat
    56881 aaaacctttc cctcttaata cagagctaag tcagtctata ggtggaaaag ttatattaaa
    56941 gacagaagat aatataacta ctgaccatat aatgccatca aatgctaagt tacttccttt
    57001 tagaagtaat ataccgtatc ttgcaaacta ttgtttcaat actgtagata cagaatttcc
    57061 tcagagagca aaagacaata atggtggatt tatagttggt ggagataatt atggtcaagg
    57121 ttcaagtaga gaacatgcag ctttagcacc attatatcta ggtgttaaag gggtaatagt
    57181 taagagcttt gcaagaatcc acaaggctaa cttgataaat agtggtataa ttccaatgga
    57241 gttttgtgat gagaaagatt atgaaaatat atctctttta gataatctgg agataccaaa
    57301 tattttagac aatctaggta gtggaatttt agaagttaag aatacaacta aaggaacttc
    57361 ttttaaggtc aaagtagaat tgtcagcaaa agaagttgac gtattaaaag ctggtggaaa
    57421 attaaactac actaaaaatc aagctaatta atatgaaaag agggaatatt gaatgtataa
    57481 agtgacactt ataccaggag atggaatagg accagaagtt gcgaaagcta tgaaaaaggt
    57541 agtagaagct actggagcag aaatagaatg ggaagaagtt aatgcaggag aggcagttat
    57601 tgaagagtat ggtactccac ttcctgaata tattatagat agtataaaaa agaacaaaat
    57661 agctataaaa ggcccaatta ctactcctgt aggtaaagga tttaggagtg taaatgtaac
    57721 tttaagacag gcattagatt tgtatgtcaa tttaagacct attaaaagtt ttaaggggat
    57781 aaaatcaaga tatgaagatg ttgatttagt ggttgtaaga gaaaatacag aagatttata
    57841 tgctggaata gaacataaaa taggtgatta tgcagctgaa agtataaaga taataacaag
    57901 aagtgcaagt gaaagaatcg ttgattttgc atgtaactat gttaaagata ataagagaaa
    57961 aaaagttaca gctatacata aggcaaatat aatgaaaatg tctgatggat tgtttttaga
    58021 tgtgtttaga gaagttgcat caaaacatgg tgtagaatat gatgatttga ttgtagatgc
    58081 agcagctatg aacttagttt taaatccaga aaattatgat gtaatggtaa tgccaaatct
    58141 ttatggagat atactatcag atttaggtgc tgggcttgtt ggaggtttag gtataattcc
    58201 aagcgcaaat attggtaaag attgtgcgat atttgaagct gttcatggaa gtgctccaca
    58261 aatagcaggt caaaataagg ctaatccaac agcacttatt caatcatcag taatgatgct
    58321 tagatactta ggtgaatatg aaaatgcaca aaaaatagaa acagctttag aaaaagtatt
    58381 tcttgaagga agtaaattga ctgtagattt aggtggctca gcttctacta cagagtttgc
    58441 ggatgaggtg tgtaagtata ttgtatagat tttatttagg gaaaaagtga aatatagatt
    58501 gaaggaaaga ggctttctca aaatagagat aagccttttt ttattggaat gataaaatat
    58561 atttgatttt atacaaaaga gagttcctca taaacgaaaa agttaatttt gggatatcca
    58621 ttttaatttt aaaattaatt gattaatacg caaagaaaga ttaaacaaat ttattatttt
    58681 taattgaact ttcaaccaaa atatggtaaa atttaaaaaa gttgaacttt caactaaaaa
    58741 taaatatatt gattatgtga aattctataa aacagaaact atttgacttt agtcatggta
    58801 ggttcagtgg aggtgtgttt gattaagact ttagatagta atatattaag agaagtaggt
    58861 actttatcta gagcagtaaa ttcaataaat gatattaagt acaaggaatt aaagttacaa
    58921 aaaggacaat ttacattttt gacaagaatc tgtgaaaatc ctggtataaa tcttgtggaa
    58981 ttatcaaata tgttaaaggt agacaaggct acaacaacca aggctataca aaaactaata
    59041 aaggcaggat atgtagataa aaagcaggat gagtttgata aaagaggata taatttaact
    59101 ccaactgata aatctttgga agtttatgag ttgataattg aagaagagaa tagaagtata
    59161 gagatatgtt ttgataattt tacagatgaa gaaaaacaag tggtaactaa gttattagaa
    59221 aaaatgagca aaaacgtaga aaatgaatgg tttaaagtaa agagatagta aataaataca
    59281 ttaacttaag gagatatttt tatgattaga aaagcgaata tgaatgattt agaaagcatt
    59341 atgaaaatta taaaatctac agtggaagaa atgaagactt ataataatac acaatgggat
    59401 gaaaactatc cattagaaaa agactttgtt tcagatatta agaaacaaga tttatatatt
    59461 tatgaagtgg atggagaagt agcaggattt atatgtctta attatgaaga gccagaagaa
    59521 tatttaggct taaattggtc atctaataaa aaagcaatgg taatacatag aatggctgta
    59581 aatccaaact ttagaaagac aggaatagca tctaagttag ttgattttgc tgaaaaatta
    59641 gctgtagaaa acaatgtatc atacttaaag agcgatacat attcaataaa ctcaaaaatg
    59701 aatttattac ttacaaagtg tggttttata aaaatcggag aaatgagttt tttaggaaag
    59761 gagaaatctt tctactgtta tgataaaata ttataaagag catttataat acaaggggta
    59821 aataatagat tttcagcatt agtaagggca taatataacc cagtatatgc tattatgaaa
    59881 gggatgattt atatgattgg atttctaaag aaaagaagaa gtattagaaa atacaaggat
    59941 gttgaagtag aaaaagaaaa attagataaa atattaaaag ctgctctttt agctccatca
    60001 tcaaaggggc ttagaacatg ggaatttata gtggtagatg ataaagaaaa gctaattaat
    60061 ttatctcaat gtagaactaa gggtggtgga tttttcttaa aaaatgcacc tttagctatt
    60121 gtaattattg cagataaaga aaaaaatgat gtttggatag aggatgcaag tatagctgct
    60181 tcatacatac aacttcaagc tcatgaacta ggtttaggtt cttgttggat acaagtaaga
    60241 aatagaatgt atgatgataa tatagaagct gataagtaca ttagagaaga attaaaagta
    60301 cctagcaagt acagtgtaga atgtataatt tctataggat attctgatga agaaaagaaa
    60361 gcatacaatg actctgattt agactataaa aaggttcatt ttaacaactt ttagtatata
    60421 atatgttgta ttaagacatt atattttttt ctattgttaa tatatactta aaattgtaaa
    60481 aatatatctg aattttacta acttatctag gggttatgta caaggatttt aaagataaat
    60541 atagaaatta atgtttataa tcgatacata acttgagtaa caaaatttaa ggaggttaaa
    60601 gtatgggtat atttaaaaca aaaatggatg aggactggaa agtaaactac attaaagagt
    60661 ttaatgaaat gagagattct tatgagagta aacttcaaaa aaaacagttt gaggtagata
    60721 gtttgaaatc agaattggat agactaagaa gctataaaaa tagccttaag ccaaaagaaa
    60781 aacaaattac tgatgatgat ataaataata taaagagttt aagaagagat ggcttaagct
    60841 ataaagaaat ttcaaatcaa acaagttgga gcaaagcaac tgtaagtagg gtattaaatg
    60901 gactttacga ttagcatata catggggtga tttaaattgg gatgaagtat agttacaaaa
    60961 aatgcataat agattctatt ttattgatgt ttataattca aatattaagg atgattttga
    61021 attatgtact tctaagtcaa tttgaattta ctcttgaaaa ttttaatatt ataaacttaa
    61081 tttcttttac attagttggt ttatcgttaa ttttattttt aaaagataat tctttgtata
    61141 acaaggttag aaatagaaaa atcacagaag cttttgaaga aaataaaaac aatatactta
    61201 ttgaaaagtg taagcttata ctttttgttg tggttttatc tttagcaata attgtaactt
    61261 attgtactaa gggatatgta ctttttaatg taactatgat gactttgtct gtattgatag
    61321 ttccaatatt tgaagagtta ttttttagag aatatatatg gaactatcta agtaatttta
    61381 taaagtcaaa gagtaaaatt atctgtataa catctatact atctggaatt tataatatag
    61441 gatacataga tgttataaga aattatgtaa tattgtataa caattcaagc tacacctttg
    61501 aagttattat ttcaaaaatt atgataggta ctgtttttgg tattgtactt ggattagtta
    61561 agtataggtt tagagatgta gggttttgta tattattgag gagcttattt gctatattta
    61621 ttaggtagat agtataatcg gtttataagg ttgttttaaa tttaatttta aatcaacctt
    61681 attttatttg tgttttataa aacacttata tattaataac taataaattt aataaagatt
    61741 atttgaatat ttttaaaata tataaatatg tattgacata taaatattat gaattacaat
    61801 ttatataaac taataaaaat taaataatat taatcaactt attgaatgga ggaataaaaa
    61861 tgaaatttgt gactttttgt agtagcaatg aagaaaaaat aggtgtattt aattcagaaa
    61921 caaatagtat atatgaaatt aatagtttag gattaagtaa attatatact gatatgaatg
    61981 attttattga aaacgtatct acaggagatt tagaaaaaat aaaaaacaat tcatttgaaa
    62041 acgcaaagtg ttataagtta gaagaagtta aattatgttc cccaattgta agacctaaaa
    62101 aagatattat atgtttaggt ctaaactata aagaccatgt caatgaaatc ccagatggag
    62161 ttataaaaaa cgtagttatg ccagattatc caatttattt ttctaagaga gcagacaaaa
    62221 taattggagt ggatgataag ataagcttac atggagattt ggtagagaag ttggactatg
    62281 aatcagaact tgcagtaatc ataggtaaag aaggtataaa tatatcaaaa gaagatgctt
    62341 atgaatatat atttggatat acaatagtaa atgatataag tgaaagagca cttcaagata
    62401 aacatgttca atggtttaga ggaaaaagct tagacacaca tacatctatg ggaccttgta
    62461 tagtacataa agaagaattt gaacatcctt taaaattaga tataagtagt gtagtaaatg
    62521 gagaagtaag acaaaattct aatacagagt actttatatt tgatatacct actataataa
    62581 atgatttatc aagaggaatg actctaaagc caggagatat aatttcaact ggaactccag
    62641 caggggttgc tatggggatg aatcctcaag tatatttaaa gcatggcgat atagttgaat
    62701 gtaaagtcga aggaatagga gttttaaaaa atatagttga ttaatggata aatatgccat
    62761 ataattttat tagtacaaat aatttaatta atataggatt gatcaaactt atgattaatt
    62821 tattatagag agggatgagt atatggcaag agaactagaa ttaaagtatg gctgtaatcc
    62881 aaatcaaaaa ccatcaaaga tttatatgaa gaatggagaa cttcctatag aagtattaaa
    62941 tggaaaacct ggatatataa attttttaga tgcatttaat agttggcaac ttgtgaagga
    63001 attaaaagaa gctacaggtc ttccagcagc aacttcattt aaacatgtaa gtccagcagg
    63061 agcagcagta ggggtacctt tatcagatac tttaaagcaa atatattttg ttgatgattt
    63121 agagctttct ccattagctt gtgcttatgc aatggcaaga ggagcagata gaatgtcttc
    63181 ttatggtgat tttatagcac tttcagatgt atgtgataag gagactgcaa ctattatagc
    63241 aagagaggtt tctgatggta taatagctcc aggatataca gatgaagccc ttgaaatatt
    63301 aaaaggaaaa cgtaaaggca actataatat tgtaaaaata gatgaaaatt atactcctga
    63361 gcctattgaa acaaaggatg tttatggaat tacttttgaa caaggtagaa atgaaatatt
    63421 aataaatgaa gaactattaa aagatattcc aactgcgaat aaaattttta cagatagtgc
    63481 aaaacgtgat ttaattatag ctcttattac attaaaatat acacaatcaa attctgtttg
    63541 ttatgctaaa gatggacaag taataggggt aggtgcaggt caacaatcac gtattcattg
    63601 tactagactt gcaggtaaca aagctgatac ttggtatctt cgtcaacatc ctaaagtatt
    63661 aaacttaaaa tttaaaaagg atataggtcg tccagataga gataacacta ttgatgtata
    63721 tctttctgat gattatatgg atgtgcttgc tgatggcata tggcaaaact tttttgaaga
    63781 aaaaccagag cctctaactg gagaaggaaa aagagcatgg cttaaaactt taacaggagt
    63841 agcacttggt tcagatgcat ttttcccatt tggagataat atagaacgtg caaaaagaag
    63901 tggggtaagt tttatagcac aaccaggtgg ttctattcgt gatgataatg ttatattaac
    63961 ttgtaataag tataacatag tcatggcatt tactaaaaat agattattcc atcattaaaa
    64021 tactgaaagt ataaagataa tatagattaa gtaaaaatct acaacaagtt ttgttgtaga
    64081 tttttttatt tgctgtataa ttttaaatag aactttattt ttagatattc aatttggagg
    64141 ttgatatggc tttaaatata agaaactata gcctattatg taatcaagga acagctaaat
    64201 ttaatgaaga tgtagtcggt ataaatccat ttggggcatg ggttttagat ggtgctacag
    64261 gacttaatgg aaaaaattta gtgtcaaatg aaagtgacgc aaggtggtat gttcagtggt
    64321 ggaataggta tttatataaa aatataaata aagaagaatc attaaagagt attattaata
    64381 ctggaataat agaagtaaaa aaagagtatg aactaagatt aaatgggttt aaggttgaaa
    64441 aattagattt accatctagt tctatagttg ttgtaaaatt tcatgaaaat aaagtagagt
    64501 attttttatt aggagattgt gctttgttta ttaaaaatgg agattccaaa gttataaaag
    64561 ataggagtat ttgtaaattt gataatactg tatttgatga aatgagtaaa ctttctaact
    64621 tggaagagat ggcatttgat gagattagaa ctagtgttat ggatacaata gtaagcaata
    64681 gattgaagaa aaatacgaaa gagggctatt ggatattgga ttttgatgag gaagctgtgg
    64741 aaaattctat atatggatac attgaaataa aaaaagattt tcaattgatg ctaacgagtg
    64801 atggttttac aagtgcatgt gatagatata atctaataaa agaagaagaa ttgatacgta
    64861 tagcagaaaa atttggtata ggatatatac ataataaagt aagggatttt gaagataatg
    64921 actataaagc ggttaaagtg ccaagattta aaataaaaga tgattcatct tgtttatatt
    64981 tagatatata taatgattag actgtataaa actatttaat tttagaaagg aggcaagtga
    65041 aaagtgaaaa ttcttcatat aataacacaa aaaccaaata gtacaggtag tggtatatat
    65101 ttgtctggta tgataaaagg gttcgaaaaa atcggtcata agcaagctgt aattgcaggt
    65161 atagatgtaa atgatgatgt aaattgtttt ccaagtgaag taagttttta tccagtaaag
    65221 tataattgtg gggagttaaa ttttcctgtt gtaggtatga gtgattctat gccatatgaa
    65281 agtacaagat ataaagattt aaatatagac atgataaata gattgaaata tcaatttaaa
    65341 gtaaatatag ataaggctat gaatgatttt aaaccagaac ttataatatg tcatcatttg
    65401 tacctcttaa ctgcatttgt tagggaaata gtaaaagata taaaggttat gtctatttgt
    65461 catggaactt gtttaagaca gttaaatacc attgatttag agaaagaata cataatagca
    65521 aatataagga aattagattt aatatttgct cttcatgaaa atcagaaata tgatattata
    65581 aagacttttg gagtaagtga aagtaaagta gttgtaattg gtagtggtta taatgatgat
    65641 atattttata ataaaaatta taaaataaag gatgataaaa taaaaatagt atttgcaggt
    65701 aagatatgta aatcaaaggg attgattcca tttataaaat ctatatctaa attaaaatat
    65761 agtaaagatt taatagaagt caattttgca ggtactggaa gcgatatcga atcatataat
    65821 gagatagtta aactggcaag taagtctcca tttaaaatga atttcctagg aaagttagaa
    65881 cagagagatt tagctgagct attcaacagg tcacaaatat ttgtgttacc atcattttat
    65941 gagggattac cagttgtagt tttagaagct ttatcttgtg gcacagatgt aatcactact
    66001 gatattcttg gtgttaaaga gtggattggt agtgagatta ataattctgg aaagatagaa
    66061 tatatttctc taccttttat ggagaaagaa ggtattccaa aagatgaaga gttatatgat
    66121 tttgaaaata atttgtataa tgcaatagat tctaagatac aatctttatt gaataactca
    66181 aatgagaaaa catctgtaga tatgtcaaaa aaaacgtggg atggcttagc atatagaata
    66241 aatgaagtta ttttaatgga tgaattgtgt ttagtctaaa attatttaaa ttataaaaaa
    66301 gtgtaactat ttttaaactt tttttaaatg attttatatt atactaatac taaatatatt
    66361 tgttttaggg aggtaattta tgaggattag taaaaaggta ttggcgttag gtatttcagc
    66421 attactgtta agtgttagtt ttccaacatc aataaacgca ctagataaga ttgaaaatat
    66481 tcaaggagta gataaatatg aaacagctgg gcttatttca gataagcaag attttacaac
    66541 agcagttctt atcaatgcag attctacaat ggtagatggg attgctgcaa gtggtcttgc
    66601 aggagtaaat aatgcagcta ttttattaac taataaggat gatataccag aagcaacact
    66661 tcaaagactg aatagagtta ctaagatata tgtaataggt ggagaaaatt ctataagtaa
    66721 agatgtagaa aaaatgttac ttatgagaag aatgcaagtt ataagaattg atggtgtaga
    66781 tagagtagat acaagttata aaattgcagg tgaaatagaa aaaattaaaa acagtgataa
    66841 gatgttttta gtaaatggat ttaaggatga agctgatgca gtaagtgttg catctgttgc
    66901 ttatagagat ggagctccta taatattgac aaaagatata ccttcagcag aagaagacac
    66961 agacttagac ccatggtttg gagtttctcc tgttccagta tatgctattg gaggagagtc
    67021 tacattaagt gattacatag tgagcagata tagagcaact agaattggcg gtgttgatag
    67081 atatcaaact aataaaaatg ttattgaaaa gttctataat ggagcaaaag agttctatat
    67141 aacaagtgga gacgatttag tttatgcttt agttgcatca ccacttgcaa aaaatgctcc
    67201 tgttgtttta gtttcaaata aaagtgataa gagtatatta agtggagcta gtaaagtaac
    67261 agcaataggt ataagtgata agagtattat agagcaatgt ttggatgctg taaagaagta
    67321 atggagtgtt attagtaaaa tgaatatata cattgaaaat tagtagattg ttcattttat
    67381 aatggtataa aatatatggg aagaggctat caaatggaga taagcctctt ttttattgat
    67441 aaaattatga aaatatactt tattttatat aaaaaaagta tctcatgaac taaatgtcta
    67501 ttttgatata ctatctcctt ttcttatatt atatgtatta tatgattttt tatatggaat
    67561 aaatttcata agtattatct tcctttaatt tttctaacca aatcatctct aaaatatcta
    67621 gttcttctaa atagtctttc tctgttttgt tatcatcatg ttttataagt tctaaaactt
    67681 caaatgtaaa tgctttttca ccatattttt tccattcatt atttaattct tttatcatac
    67741 atgagccagc accaagctga aatttgagag agttaaattt gctttttatg tcattacttt
    67801 ttccaaggta aatcttttta gtaatatcat tttttattat gaatacacct gtgtcaaatt
    67861 tcatttcttt gtataattgt tttaattcac gttttctatc catgatttta gatacctctc
    67921 tttataattg atatgtaata tgtaagttta aaaatatatt acatatcaat tataaagaga
    67981 ttaattttat atgtaaatag tttttataaa tttaattatg aattagtcat caataaacaa
    68041 gaaatttagc tgtagaaatc cattataaag gctattataa attttaatat gctaaaaata
    68101 taaaaagaat agttgacagt atagaaaata tggaataaac taaaagtgta atagtaatac
    68161 gaaattatat attacattga aaggtgtgaa tgattattat gaataaaaaa atagaattaa
    68221 tagggaattt tgcaagtgat tggattaggt atagtgatta tgaatataaa attactgaag
    68281 aagatgaatt atatatagtt ccaacagaag atgctgtgtt taccatgtat aacccatttg
    68341 atgtagcaga tgatataata gtagatataa ttcgcatagg acaagaggca ttacaagata
    68401 tatcaaaaga gacaaaagaa atgaaactaa agaaagaagt acttgaattt gtaaaaaagt
    68461 atggattatt gggtttaata tattcaagtg tatataatag agatatagtt ggagaagaaa
    68521 gagtactgat gattgaaaag aattatatta caaaagagaa tatattgagt acagatgaat
    68581 atgtaaacat gtttatacca tttgcaagtg atgaagatgt gtattttaaa aaatatagaa
    68641 ggggtataga tgtatcaaag agagaagaat ctccaaagtt ttttggaaag agaccattga
    68701 ttttagattt agtattttca aagttctaca ctgaacaact agattggatt gtaagttttg
    68761 ctaaagatat gtgtaagcat tttaatcaaa tttgtgtata taaaaataca tctaatctat
    68821 taactgaaag tgttactatt atgtctggag ggtttaatcc tcaaaagata ggattttcta
    68881 taagccaatt agataaacca attattgcat gggagtttga ttctttgaaa acagctattg
    68941 agactatcta tgcctttgca attacaagtg aaccttcttc tttaaataaa tgcaagtact
    69001 gtggtaaaat ttatattcct acaaattcta aatctcaata ctgttcacct tcttgtagaa
    69061 attgctcaaa tgtaaagaaa agtagaagta gagccaaaaa agataaataa aattaataaa
    69121 tatatcattg tttagatttt agtaaataac tactgaatta aaagttgtta tagaaaatta
    69181 tatatctaaa tgatggtatt tttttgttga gaaaattgaa aagtttaatt gagatagtaa
    69241 ttttgagttg acaaaaacca cttttaacac tactattata agtggttggt aaaataatat
    69301 acactaattt aaatatagca taaaaaacaa aaaaagtcaa tgaaaaaaat aaaaaaggag
    69361 aatttaaaaa tgatatcacc tattattttg agttctataa atcagaattt aaaggaaata
    69421 gaaagaaatg aactgcttga aactaatata gaatctggag attatggtct agctctttct
    69481 gaatctgatg taaaagatat aataaattcg agagataata ctttaaaggg gtatggaaga
    69541 atagaattag atataaaagt tacaaaacag ttaatagaaa atatatatac atcacaatat
    69601 acaaatgtgg acaattattt agaggctatt aatgatatgc aagaaatttt ttattattta
    69661 aaaaatgaaa ctgatgacaa gatatgtgat gatgagatta tagaaatact gggtgaattt
    69721 tatgaaaaat tttcaggcaa tatggacaat gtaagaggag aagcagacga atttgcaaag
    69781 aaatttaaat ttggagaagt ttaatatatg ataggagaga ttttgtatgt taaataattt
    69841 aagtaatatt aatataaacg aaaataattt aataaagaac caatattcca tatctctact
    69901 taaggaatgt ctaaattgta aagtgataga tgaaagagaa gtatataata tacaacaaga
    69961 aatatctcta atacttatgg atttgattaa aaaatataca aatggtcaaa gcacatcagt
    70021 aaaaacagaa gttgccgaaa aactgttgat ttcaatatgg tatgctattg atgcatatat
    70081 aaataagctt ggagatatag aaaaaagagt tgtggagttg actgctaaga atgttaaaaa
    70141 agtgtacctt ggaggcattg aaatattaga ggaagagtta gttaggctga aagattttta
    70201 taaactaatg atgagtagaa aattggatac taattcaata gcatataatg ataccttgat
    70261 ggagttatca agtttcttta aaagttatat tgtaaaattt gaagctcagg acgttccagt
    70321 aagtatagat tatccattgg cactagatga tatggatgtt aagggtattt attatgtgaa
    70381 gaattatatt gagagtatag atatagaaaa tagattttgt aacctattta agaaaaaaga
    70441 tagagaaaaa ttattttatg actatggggt gatttataaa atagattgtg atgttatact
    70501 tgtaaattta tttgaactta ttataaataa ttgtattttt tctactattt taggaaatga
    70561 agtgattaat ttagaaattt caaaatatga gtatgaattt ttagaaaaac aatttaaaaa
    70621 tatgtacaat aatattactg aagataaagt tataggctct gaagaaagta atataattga
    70681 attgattgaa gtagatgata gttttaacac tgaagtagat agtaatttgg attataatga
    70741 ttttgaagaa attaacagag aaattagaat aaataaaaaa ataactttac tacttatgga
    70801 agcaattgaa gtattaataa aaagattaag catagaagat gaagatgtaa taagatatat
    70861 taaaaaatat gaaaagatat ttattgaatc tgtgctaagc tcaataaaaa gtaacacttt
    70921 taaaagtatg gtaactgtga gtaaaaataa aaatgaacac ttagaagact atattgttga
    70981 tgatgaaagt aaattggatg atgaatcgtt tagagagata ttcaatgaga tattagatag
    71041 tacatctata attgaaaaaa taagaataat aaaggaaaat atcaatgcta aaaaagactt
    71101 tatcgatatt cttgaatctg aatgtttatt tggagaagag tatttaatgt tgtttgcatc
    71161 attaagtgaa ttagaattgg caatattggg tagtgtagta ttttatgaag atattagaat
    71221 gagagaaatt aatatattag aatttatgtt aaatgagaaa actgaaacta ctttatggaa
    71281 gacagagtac attaaattta tgaagaggca aagtgaagat aagatatact ccatagaaga
    71341 gcatatgaat aaaattaatt aacaaaaaat tgtgaataaa tagaatgtta gtatataata
    71401 taatctgtaa agcatatcaa aaatacatat atttggagga aaattatgaa agagtatatc
    71461 ataaataatg ttgaagaaat aagagaagaa ttaattgatt tatctaagaa aatatgggaa
    71521 aatccagaat tagcctttga agaaaagtat gcaagtagca ttcaaaaaga atacctaaag
    71581 tcaaaaggat ttaaaattga agaagtagaa aatttaccta ctggatttat agcaagtttt
    71641 ggagagggta aacctgtgat tggcatactt ggagaatatg atgctttacc agagctatca
    71701 caatgtgtga gtgcagaaag aaagccattg gtagaaggaa aagctggaca tggatgtggg
    71761 cataacctgt tgggtgtagc aggtgtagga gcagttgttt ctataaaaaa attaatagaa
    71821 gaaaataaat ttaatggaac aataaaatat tttggttgtc ctgctgaaga agaaggtgga
    71881 ggaaagacag ttatgtgtat aaatggctgt tttgatgatg tagattgtgc atttacatgg
    71941 catccttttg atataaatgc accttggaga ggtggaagtc tggcaaactt atctgtaaaa
    72001 tttaaattta aagggataac agcccatgca gctcaagctc ctcataatgg tagaagcgca
    72061 cttgatgctg ttgaaattat gaatgttgga gctaattatt tgagagaaca tgttattgac
    72121 tctataagaa tgcactatgt tataactaat ggtggtggaa gacctaatgt agttcctgga
    72181 tttgctgaaa gttggtactt tattagaggt aaaaaagcaa gggatgcaga acatgttctt
    72241 gatagattaa taaaagtagc tcaaggagca gcaatgatga cagaaactga aatggagtat
    72301 aaggttacag atggaatata tgactatata ccaaaccaat gtttgactga tttagtttat
    72361 gacaatatgg tttttgttgg ttgccctaaa aatactcctg aagaagaaga atttgcaaaa
    72421 aaattgtgtg atactcttac tagagaagaa agattaggtg tagcaacatc attacaaaat
    72481 gataaatcta ttgtagaaag ttatatacac aaggaaatag ttgacggtga caaagataaa
    72541 ggactagctg gttctactga tgttggagat gtaagttatg taataccagt ggctcaattt
    72601 gcaatggcag cttggcctgt tggaattgca agtcatacat ggcagtcatg ttcatcagca
    72661 ggttcaaata taggattttc tgctatgata aattcagcta aagtactggc ttgtagtgct
    72721 tatgatgtat ttatgaatac taaaattatt gatgaagcaa aaattgaatt tgataaatca
    72781 ttagatggac aaaaatttaa accattggta taattttggt aataaaatat ttctgggggg
    72841 aatagctatg agagaaaaag ttgaaaaagt gcttgaggaa aagataaaac cagtattaca
    72901 aagagatggt ggagatgtag agcttataga tgtaaatgaa aatggtgtgg ttttagttag
    72961 actacaaggg gcatgtagtg ggtgtccagg agcgactatg acaatcaaag ctattattga
    73021 aaatgttttg gtgagtgaag ttccaggagt aactcaagta ttaggtgttt aatagttagc
    73081 gtttattaaa tgtatgttat acataagtat ttaataaaga agtaaaaaaa atagtgaagc
    73141 aatatatatg aaaaagtatg ataaagataa taaaacatta tagaaataat gaaatatttc
    73201 atatgtatat gaagaaataa gaaaaaatag tgaagcaata tatatatgaa aaagtatgat
    73261 aaagataata aaacatttat agaaataatg gaatatttca tatatacata tgaaaaaata
    73321 gtaaaacaat atatatatga aaaagtgtga gaaaataata aaatactata gaaacaatta
    73381 aatattctat ataggtatta agaaacatga aagaaataat ggagtattat agataaatat
    73441 gtaaaagtgt gaaagaagga tgtagataaa tttgtcttat acaaatttac ctacagtatt
    73501 tcttacacac tttttatttt actatccaac cataccaata aacataccta taaagtatgg
    73561 aactaaccat atagtccata caactatact aaacttatga aagaattttt tcttttcctc
    73621 atcatctttg tataggacaa aagtagccca acttgcatga aaaagcataa gcacaattgc
    73681 aagaagacct gtaatactat gaaggttttg tgataaagca gattttactt caaaagtatg
    73741 gctgttggct atgttactca ttgtaaatgt tcctaaagta tcaaatataa acccaagcca
    73801 aaatataatt acatgtttct tttttaaaat ttttgccttt ctttctccaa atacacctat
    73861 agtgtagaaa attaaagctg atgttataaa tactattgca agtattaatt tataattcat
    73921 aattatctct cctttacttt tgccaatata tttctaagat tttttactaa gtcactcaat
    73981 tcgttttcat cacaattttt aaaaatatca tcaaaacttt cttgcatttt aattttaaaa
    74041 tttgtggcaa attcaaatcc tgttgttgta aacttaacgt atgtatttct tttatcatta
    74101 gatttcttga atttttctat atatccaatc tgttctaatc tgctgattat gcctgaaacc
    74161 gtacctttag ctaaagacat ttcatcacat agttgtgata ttgtaagttc ttgattatga
    74221 gctaccagtt ttataactat aatctgttgg tgtgttaatc cattttcttt aaaattttct
    74281 tctactgtgt acatagtttt agaataaagt tcttttaaga gcatggctat tctaaaacag
    74341 tcataattac ttttcaataa aatcacctct taaatggttt gcatacaaac taatataata
    74401 tacttttctt atatagtcaa cctaaaattt tataatttta tagaaaataa tgaagaatag
    74461 aacataaaaa ttttatttta ggtataaata aaataatttg atgaaatttt aacaattttt
    74521 aaaaagtttg tttacacagt taataaatga tgctaaaatt aactcataga taaatataga
    74581 aaatttaatt ttgtttaatg ataatggaac cacgaaggtt ttaacgttac tttacgtttg
    74641 cttttatgtg gtttttttat tgcaacaaag gggggttggg gtttgttaaa ggatatactg
    74701 aaaaagttgt tgcagtttat tttagtaatg tttttattat cttttattgt tttttacatg
    74761 gcaagacttg ctccaggaga cccattaatc tcttactatg gggatggagt agaaagaatg
    74821 agtacacaag aaaaagaaaa tgctatgaaa aaacttggtt tgaatgaacc aatatacagt
    74881 caatatataa aatggatttc aaatgcatca aaaggggaat ttggaatttc atttaaatat
    74941 aaacaaaatg ttacttctgt tataaatgat gtctatataa acactataat attaggtggt
    75001 tcagggtata tacttacctt tgtgctagcc ttattgttag gaatattttg tacattacat
    75061 gaagataagc tcattgatag aatcatatgc aaattaggaa caataaccaa ctgtattcca
    75121 tcattttggg ttgccttagt actcatactt atatttagta taaacttaag tatacttcca
    75181 agtagtggtg cttattcaat gggagaagag tccagtatat taagtaggat atcacatcta
    75241 atcttacctc ttacagtact tatattaagc catctatggt attacacata tatgataaga
    75301 aataaattac tagaggaaat aagagaagat tatgtgcttt tatgtaaagc aaaaggttta
    75361 aataatagaa ctattgtttt taagcattgc ctgagaaata taatgccatc atatattagt
    75421 ataatggcca tttcgatacc tcatatacta ggaggaactt atgtagttga aaaagttttt
    75481 tcttatcctg ggttaggaac actttgtttt gaaagtgcaa agtatcatga ttataatatg
    75541 ttattagttt tatgcttaat aacaggggct ttagtagtat ttgggaatat gttagctcaa
    75601 attataaaca ataaaataga ccctagaatg aaatatgata gaggtgatac taatgaagcc
    75661 acaatatagt gattttgaaa ttgtaggaga aaattatatt ttagtagcag aagatgaaca
    75721 tatagaaaca aagaaaacct tatctaaaaa aattaaagaa ttaccatata tatcaattat
    75781 tattttatca atcatagtca ttggaagtgt attctcatct tttataatga cacatgaacc
    75841 aacatacatg gatttagcaa gttctaattt agctccaaat aaaacttttt tatttggaac
    75901 agactcaatg ggtagagata tatattctat gatatggtat ggaggaaaaa tatctttatt
    75961 tataggacta ttttccactg taatatcaac tgttatagga attgtttatg gtagtataag
    76021 tggttcagca tctgaactcg tagatgatgc aatgatgagg tttacagaaa tcatacttag
    76081 tataccttca atattaatta taatatttgt acaagctata cttggaaatt caaatccagt
    76141 atctatgtcc atagtaatag ggataacaag ttggatgaat atatctaaga tagtgagaac
    76201 agaagttaga caaataagaa atagcgaata catactagca gctaagagca tgggaggagg
    76261 attcttctat gtattaaagc aacatttact tccaaatttt gtggcatcaa taatgttcat
    76321 ggttgtaaca aacataggcg cagctattgg tactgaatcg acacttagct ttttaggaat
    76381 tggtttacca atagaaattg tttcttgggg aagtatgtta tctttgtcag aggaagcatt
    76441 actttcaaat agatggtgga ttatactgat tccgggtata tttttggtta ccacattggt
    76501 gtgtataaca aatataggta attatataag aaagaataat aataaaaaat ctagtaattt
    76561 ataaatcagc atttgagtac taaataagca aatactgagg tatttgaaat atctatttaa
    76621 ataaatgggg tcaaagcttt gaaaaatatt ttataaaaag caaaaggggg agcatcatga
    76681 aattaaagaa gttaaaagtt ttaagtttag taatgatact tagtctaatg gcaggttgtt
    76741 ctagtggagg agacaaagat aagaaggcag atacgcctaa agatggaaag gtacttgttt
    76801 atggaagtaa tgactataca agtataaatc ctgcactata tgaacatgga gaaataaatt
    76861 cattaatatt taatggatta acagctcatg atgaaaataa taaggtggtt ccttgtctag
    76921 caaaggactg gaaatttgat gaggcaacaa atacatatac ttttaattta agagatgatg
    76981 ttaagtggca tgatggagag aaatttacgg caaatgatgt taagtttaca atagaaacta
    77041 taatgaatcc agataatgct tctgagatag catcaaatta tgaagatata acaaaaattg
    77101 atgtggttaa tgataatact ataaaaatta ctttaaaagc tccaaataca gcaatgcttg
    77161 attatttaac agtaggagta ttaccaaaac atgttttgga aggtaaggat atagctactg
    77221 atgaatttaa tcaaaaacca ataggtactg gaccatttaa acttgaaaaa tgggacaaag
    77281 gtcaaagtat aacacttgtt aagaatagtg attattttgt aaaagaacca ggattagata
    77341 aagtagtctt taagattgtt ccagatgata aggctaaggc aatgcaatta aaatcaggag
    77401 aattagactt agctcaaata actcctaaag atatgtctaa ctttgaaaaa gacgaaaaga
    77461 attttaaagt aaatataatg aaaacagccg actatagagg tatattatac aattttaatt
    77521 ctaaattctt taaagataaa aaagctaaag gattaccaaa tgctttaagc tatgcaatag
    77581 atagaaaagc aatagttgac agtgtattat taggacatgg tgtaccagca tattcacctt
    77641 tacaaatggg accatataat aatcctgata tagaaaagtt tgaatataat ccagaaaaag
    77701 ctaaacaaga aattgaaaag ctaggttgga aattaggttc tgatggaatc tatgaaaaag
    77761 aaggtactaa gttagctttc gagataacag caggtgagag tgaccaagtt agagttgata
    77821 tggctaaaat atgtgcacaa cagttaaaag aaattggtgt tgatgctaaa gctgttgttg
    77881 taactgaaac agattgggct aatcaagatg ctcatttaat aggttgggga agtccatttg
    77941 acccagatga ccatacatat aaagtatttg gaacagataa aggtgcaaat tacagtgctt
    78001 actctaatcc tactatagat aaaatacttc aaaaagcaag agaaacagaa gataaagatg
    78061 aaaaattaaa gttatataaa caattccaag tagaaatgac taaggatatg ccttatacat
    78121 ttattgctta tatagatgca atatatgttg gaaaaccaaa cataaaagga ttaacacctg
    78181 atacagtttt aggtcatcat ggtgtaggta tattctggaa catagctgac tggacaattg
    78241 aataatactg aaattaatta gaaaccttag ggataaaaat atatccctaa ggtttgaata
    78301 gcataggggt tgatggtttt tggaacattt attggaggtt aataatctat ctgtaagttt
    78361 taaagtagaa gaaggagaag ttcaggctgt aagaaatgta tcttttaatc taaaaaaagg
    78421 tgagacattg gctatagttg gagaatctgg ttgtgggaaa tctgtactgt gtaagagttt
    78481 gatgaggata ctcccataca atggatatat taaaaatgga gaggttttac ttaaatcaag
    78541 tgatttggtt aagaagtctg aaaaagagat ggaagatata agaggaaaga atatatcaat
    78601 gatatttcaa gaccctatga catctctcaa tccaactata tcaataggaa agcaaattgc
    78661 agaagctgta atcattcatc aaggtataag taaaagtgaa gcaaaaaaga gagccataga
    78721 gcttattgaa ttggttggaa ttgataatcc tgagaaaaga tttaaacaat ttccacatca
    78781 tttttcaggt ggaatgagac aacgtatagt aatagctata gcacttgcct gtaatccaga
    78841 tgtattgatt gctgatgaac ctacaactgc tttagatgta actatacaag ctcaaataat
    78901 tgatttaata aaggatttac aacataaaat aggtttgtca ataatattta taactcatga
    78961 tttaggagta gttgcaacta tagctgatag aatagcagtt atgtatgcag gaaagattgt
    79021 agaaatagga actgtagagg atatatttta tgaccctaga catccatata cttgggggtt
    79081 gttgggttca ttacctactt tagattcaca ggatgaatat ttatataata tacctggaat
    79141 gcctccaaat ttattgaacc caccaaaagg agatgctttt gcaataagaa ataaaaatgc
    79201 acttaagata gattatgaaa aagagccacc aatgtttaaa attaatgata cacatagtgc
    79261 agcaacttgg ttattgcacc cagatgcacc tgaagtagat gtaccagtta gagtaaattg
    79321 tggaagggtg atttctaatg aatagagaaa aaatactgga aataaaaaac ttaaagcaat
    79381 attttcattt ggacaaaagt actactgtaa aagcagttga tgatataagt tttgatatat
    79441 ataagggtga gatatttgga ttggtaggag agtcgggttc aggaaaatct acaactggta
    79501 aaactataat aagactacat gaatctactg gtggagaagt aatttacaaa ggaaattgca
    79561 tatcagataa aaaaacgtac aaatttataa aaaaagatgt aaacaaaagt atgcagatta
    79621 tttttcaaga ttctacttca tcattaaatc cacgtatgac aattgcagat ataatttcag
    79681 agccactaaa aattcaaggt atctgtaaaa ataaaacaga tagaatgaat aaagtatatg
    79741 agatgctaaa gctagtagga cttgatagaa gttatgcaaa taagtatcca tctgattttt
    79801 caggtggaca aagacagcgt attggcatag cacgagctct ttcagtagac ccagaattta
    79861 taatagctga tgagcctata gcttctttgg atgtgtctat acaagcacaa atagtaaatt
    79921 tatttaaaaa attgcaacaa gaaaagaatt taacttgttt atttatagct catgatttat
    79981 ctatggtgag acatattagt gaccgtatag gagttatgta taatggaaaa ttagtagaat
    80041 tggcagattc aaatgaattg tataataatc ctattcatcc atatacaaaa tcactacttt
    80101 ctgcaattcc tgttcctgac cctagatatg ctaagtcaag aaatagaata gagtataatt
    80161 ctaatggata tgattgttca aatgaaaagt cattaagttg gattgaggtt tcagatggac
    80221 attttgtata ttcttctaaa tcagaaataa ataaatatca acaaaattta aaagttgttt
    80281 agtataaaaa tacttgaaca ggtatttatt tgaaatacaa actaaaaccc tatttttgga
    80341 gaaattttaa aactactcca aaatagggtt ttttatttct acttttcaaa tataataaat
    80401 tcaaatataa taaaattaaa aacaagtaaa gtatgttgtt aagaaagtgc tataaaatta
    80461 aaaatagtaa tgtagaaaaa atgagcaata atcttatttt ttctacaata attttctata
    80521 atttaaagta aggaaagttt gataaattat aaccttagtt aaagaataag agtttattaa
    80581 aaaataaagt gtgtataggg gttttagtta ataaaatatt ataatgtagg ggagcttgta
    80641 atgatatata aaaaagataa agtaatgaaa gttttagaat catcatatag ggaaaatgaa
    80701 aaagggatta cagctaacca attatcagaa aagacaggaa ttgcaagaaa caatataagt
    80761 acatatttaa accaattatt caatgagggt atggttacga agataaaagg aagacctgtg
    80821 tattatatcc ctgcaaatct taaccttaac aagaatgaat tgcttgatac acaaagtgag
    80881 ttgcttggca taaaaaaagg aacaaaagta gaaaacaaaa taaaaaaaga tagttttgaa
    80941 acactaatag gtaaagaaca tagtttaaga cctataatag aaaaatgcaa ggtcgcaatg
    81001 ctctatccta caaatggact tcatactatt atctatggag aaacaggagt aggaaagagt
    81061 atgattgcta gatatatgta cgattattct atagatagtg gaattagaaa aaataaagct
    81121 ccttttgtaa catttaattg tgctgattat gctaataatg cacaattact tatgggacat
    81181 atttttggag tagaacaagg agcttataca ggtgcagaat cttccagaaa aggtcttttg
    81241 gaaattgcaa atggagggat actattttta gatgaaattc atagactacc agcagaaggt
    81301 caagaaatgt tgtttacttt tatagataag ggaacattta aaagactagg agatgcaacg
    81361 aaagaaagag attctaaggt attaataata tgtgctacta cagaaaatcc gacatcaaca
    81421 ctattagata cattcaatag aagaatacca atgaaaatag aaataccatc actgagagat
    81481 aggacaatga ttgaaagaat ggaattagtc aaaaattttt tcaaggaaga atcaaaaagt
    81541 atttctattt caataaaagt acataaagaa ataattaaat cactattact ttatgattgt
    81601 aaaaataatg taggtcaact tcaaaatgat ataaaattag ctgttgcaaa tggataccta
    81661 agatataaaa gagataataa agaaccagtt tatatagaga aaaaatattt tgataaatct
    81721 ataaatgaag tgagtgatga ttatagagag aagtctatta aagcagatga agttataaca
    81781 catggaatag aatattttgt atttacgtca ttaggagaag aagaagttat taactacaat
    81841 gatataaata ttattaatgc agtaaatact aatcttaata aatctgtaga aatgaatata
    81901 gcagatgctt ctctatttaa taatgttaag tttaaaagca tgtgtgaatc aataagacaa
    81961 attattaaag aagatgaaga aatagatata agtgaatcaa tatttaagtc aatggcaatt
    82021 tatataaaaa gcgtattgga taaaagtagc tctacaaaca acatagattt gaatcaaatt
    82081 agaagaaata acagaagtga atttaaaaca gcactaaaaa tagttggaat aattgaaaat
    82141 gaatttgatt tatttttatc tatagaaact gtagcatata ttacattatt catagtacaa
    82201 gctaaacaag atgaacaaaa taaaatagtg aataacatta atattatagt tgctatgcat
    82261 ggagaaacta cagcaagtaa tggtaaaagc agttggtgat attattggaa agtgcagtgc
    82321 tatttctttc gatatgaagt tagataaaag ctatgatgaa gttattgtag attttaaaaa
    82381 tttaattaat aatatagaca ataaagatgt attactattt acagatatgg gttcattaaa
    82441 ctcctttgat gaaattataa agaaagaaaa aaagtgtgaa gtaagagtaa ttcctatggt
    82501 gacgacatta actgtgttag aagcagttca gaaggccaat atgggattac ctttaaatga
    82561 cgtatacaac tctatcacta acacgagaaa gtattatttt ggaactaatg aaattcaaaa
    82621 taaagaaaat ctgtctaaaa caattattat agcatcacat gtttcagaag gtgtagataa
    82681 taaaacaaga aaaatattag aagaaaagat gagtaggtac ttagatggaa tagatattat
    82741 atctgtccca tataaaacag aaaaagattt aagtttaaat attacaaaac ttaaagaaag
    82801 tagtaatatt gttgcagtaa tcaatgaaca aagaattaat attagaggaa ttgactatat
    82861 tagcaaaaaa gatatagata aggatgaaaa tatcaataaa ttaaaaaata ttataaagat
    82921 aagtataggt tatgatgatg tagttgaagg tttaaagaca tccttaaaaa gctctaatta
    82981 taataggata tttaaagata taaaatatgt gtctgatgaa ttgtttttag tatttaatat
    83041 agaaaagaaa tacgataaag taattggact tatgatgcat ttagctttca tggtagatgg
    83101 gttgattgga aatactagag aaattgaaaa gcttgataaa gaaaaaacac ttgattatca
    83161 caagagccta agcaaaataa aagatatagt atctcaatta gataaaaaat ataatataga
    83221 aattaatgaa aaagagtgct atcaaatatt attaatatta gaatatgcag aaataatcga
    83281 aaaagattac caatgagtat aaaaacttat ttggtaattt tttttagtct aaaataatat
    83341 taatattaga cacatataaa tatacttaga caaagtatct aagttttgat gataaaaatg
    83401 attgaaataa cagtgtttta aagtgtctaa aaaaactggc ataatacttg cttatattat
    83461 taaatgagta atataaataa ttaaaattta aataaaaagg agtaaataat atgcaagata
    83521 aaatattaag agataaaaag gcaaatgacc catgggcaag aatgactaca agaaacggat
    83581 ttagtgcaga cgaaataata tcgtcatttc aaaaagctat aagaagaaat atggtggaag
    83641 aagcttgtga atttgcttat gaactatata tatcatcacc agaattagaa gataagctat
    83701 ggagaaggat acttacaata tcagttgaag atataggaat gggggaccca tcagctgtaa
    83761 taatcataaa taatttaaat caagtaagaa aggaatattc atatgcagat ggagacagac
    83821 ctttattttt catacatgca ataagatatc tttgtgcatg tgagaaagat agatcaagtg
    83881 atttattaaa aaatataatt ataaaaggat ttgcaatggg taaagttcca gaagttatgg
    83941 acgtggcact tgacaagcat acatatagag gattagaaat gggaagggat tcattccact
    84001 ttttaaatga agctagtata gtaataccag aaaaagaagt agataatgat tataaagaaa
    84061 gatacttaaa aatattagaa aaatataagc aagaagaagt tatagatacg gctttcaagt
    84121 ttaatcattg gcagtactaa gaattagagt aaatattaat aagaattaaa ataaaataat
    84181 aagaattaaa gtaaaaactg ataggaatta gagtaagaat tggctgataa aaaattagaa
    84241 aataaaagag gttagatatt gtaggagggt aattatgaat attttattag tatgtgcaaa
    84301 tggagcttca acaggtgtac ttgtggaaaa gatgaaaagt ttttgtagtg agcatgaaaa
    84361 gttaaaaact aaaactataa atatagaggc aacatcattc gaaaatttaa aatcatacat
    84421 tgaagctaat gatacagatg tagtacttgt agcaccacaa attaggttca aggaagatga
    84481 tgtagttgaa gcttgtaaga attacaaaat agcagttggt ttaatagata ctaagcatta
    84541 tggtcgtatg gatgctcctt ctgttatgaa gtcggcaatc gaattatata aaaatagata
    84601 attgggggga ataaaaatgg aagcacttca aaataattta agaagatttc ttctaccaat
    84661 agcacaaaag attgaaaaac aaaggcatct tcaagctata aaagaaggta tgataagcat
    84721 taccccaata ataattgttg gttctttaag tatgttattt atggcattaa acaatatgtt
    84781 acctgaagga agtgcaaaaa cattattatc tgaaaatatg gatacattac ttatccctaa
    84841 taaatttact atgagtttat tatctatata ttcagcattt tttatagctc aggcactagc
    84901 taaaaaatat aatcttaatc atgtagaaat aggtatgaca gcagttgttg cacaacttgt
    84961 tgtatgtgga caagtagtag atggtgtatt agatacaagt tatttagatg cacaaggttt
    85021 atttgtaagt atattagtag cacttttagt agttgatatt actaagttta tgaatgataa
    85081 aaatctcgta attagatttc caaaagaagt acctagtgta gtaaataaaa gttttagaaa
    85141 tttaactcca atgatagtat gtataatgtt atttacagca atagcagcaa taactaaaaa
    85201 tgtctcaggt caaccattac cagctattat aatgaatttc ttagcaccag ctataagcag
    85261 tgtagataat gtttttgcag taactataat actatttata actcaattat tatggttctt
    85321 tggccttcat ggagcagcaa taacttcaag tatatggatg ccaatagcag ctacatatat
    85381 ggcagaaaat gcaacactga tagcagcagg aggagaccca aaatatgtgt ttacaatagg
    85441 attttactat ggtttcttgc aagtaactgg ttcagggata actttgggat tagtgtacct
    85501 tatgtcaaga agtaagtgta agagttttaa tagtatggga aaagttgtga ttttaccatc
    85561 actatttgga ataaatgagc cagtaatatt tggaacacca atagttatga acccatatat
    85621 gtttgtacca tttgtatttg gacctgtatt ggttggagca cttaatttta tggcattaaa
    85681 agtaggatta gtaggattac ctattgcaga accaccaggc tttttacctc caggggttgc
    85741 agcatttttg atgacattag attggaaggc aatcgtatta gtatttgcaa gtataatact
    85801 tatgaccttg atatattatc cattctttaa gataatggag aaggaagaat taaataaaaa
    85861 tgcagaatta gctacaacat tagatgatga tagctttgat ttttaacaag tcattagaag
    85921 gagaaggata tggtacctga aatggaagaa aaagtattta gtataatatc agaagcagga
    85981 gatgctaaaa gtgatgtaat gatgtctctt aaggaaataa aaaaaggtga ctacaataaa
    86041 gcaaaacacc ttttaaattc agcctcagag aaaatacaaa cagcatcaaa gcatcattta
    86101 gaattactat caaatgctat gaatagtgaa gatagtggga ctgatttttt agttgtgcac
    86161 tctgaagacc attattcaaa tgcattattt gctcattctt tagtatctga acttgtagat
    86221 atatttgaga tgatggaccc aagaattaat aagaattaaa tttcttgtaa atagtgcttg
    86281 aagtttggct atagtttatt aaatcaatag atttaataaa tgaactccct tttactttaa
    86341 agtactagta tttataaatt tatttataaa tacagtaaat ttttaaatag aaaaggagat
    86401 attattatgg aaaaattttc agtagtaata gctggaggag gaagtacata taccccagaa
    86461 atcatattaa tgttgctaga taacttagat aggttaccat taagagcaat caaattatat
    86521 gataatgatg aagaaagaca aaataaggta gctaaagctt gtgaaatact aataaaggaa
    86581 aaggacccta atatagagta tttagcaaca acatgcccta aagaggctta tacagatgta
    86641 gatttttgtt tagctcatat aagagttgga aaacttgaaa tgagagaact agatgaaaag
    86701 atacctctaa aacatggagt agttggtcaa gaaacttgtg gaccaggtgg aatagcttat
    86761 ggaatgaggt ctatagaagg tgttcttgaa aacatagatt atatggaaaa gtattctcca
    86821 aattgttgga tgttaaatta ttctaatcca gcatcaataa tagcggaagc tgttagaagg
    86881 ttgagaccta attctagggt aataaatatt tgtgatatgc caataggtat ggaacataat
    86941 atagcaagga ttgcaggact caaatctcgt aaacatatgg atataagata ctttggatta
    87001 aatcactttg gttggtatac ttcaattaaa gataaaaaag gaaatgaact tttaccaaaa
    87061 ttagtagaac atataaaaga atatggtttt ataaatggtg aagaaggaat gaaaaatgac
    87121 aagaaagata gttggtttga aactaattta tttactaaag aaatagtaaa aacagattta
    87181 actacaatac caagtagtta cttaaagtac tatctatttc cagattatgt tgtaaatcat
    87241 tctgatgtaa attatactcg tgccaatgaa gttattgatg gaagagaaaa agaagttttt
    87301 ggttcatgtg cattgataga aaagcaagga cattctaagg gcagtgggct taagatagga
    87361 atacatgcag agtttatagt ggatttagct acagctctag cttttaatac acaggagcgt
    87421 atgttattga tagttcaaaa taatggagct atagaaaata tacaagatga tgctatggtt
    87481 gaaattccat gtatagttgg aaaagacggg tatgaaccat tatcaatagg gaaaatacca
    87541 acattccaaa aaggtttaat agaacaacaa ttagcagttg aaaaattagt agttgatgct
    87601 tggattgaga agtcttataa taaattgtgg caagcactta tgctatctaa gacagttcct
    87661 agtgcaacag ttgcaaaaga attactagat gatttaatag aagcaaataa aaattattgg
    87721 ccaaaactta attaagaata atatattttt aaagttccta cagtttagta catagcctag
    87781 ttaatgtatt tgtagggact ttatttattt acaaatgaga tacaagtggc aaacatagtg
    87841 tattttgcag ttaggaaagt aggtttagtt acttgttagt tggattaaat ttaggtacaa
    87901 taatttacta tcaatcaata gaaataacca gagaagttgc ataataaaaa atttacactt
    87961 ttaacaggat ttatgtaggt gtttatagaa ataaataaat atgtacataa atacataatt
    88021 atttttaagt ctaaaataca agaatggaga gatggctatg aagtggagag attatgctga
    88081 tgatgtaaat ttatttgagg attttgataa aactatcaag cctctaactg acgaacaaag
    88141 aagaaaaaat ataaacactt taattgcata tttttcaaaa gaagttcctt ctaaaatata
    88201 caatttatca aatgatgaaa ttaagcctag agatatactt agaggacttt tgaatgtata
    88261 tccaccaaaa gaaattgctc cagagatact taatatgtta cataatttat tgttgattga
    88321 atgtgaagaa agagagttag tagatgtaaa tgatatagaa gaagtagagg aagggattgc
    88381 catatggaga ggtaatataa cgaatttaag agcagatgca atagtaaatg ctgcaaataa
    88441 caagttatta gggtgtttac aaccattaca tctatgcgtg gataatgaga tacattcatg
    88501 cgcaggtcct agacttagag aagattgtga caaaataata aaaaaacaag ggcatttaga
    88561 atatactgga gatgctaaga taactagagg ctattgttta cctgcaaaat ttgtagtaca
    88621 tactgttgga cctattgtat ctggtggtca gccaagtaaa gagcaagaaa agcaactgct
    88681 tcattgttat aaatcatgtt taaatacaat aaaagaaatt gatgaaataa aaaatattgt
    88741 tttttgtgga atttctacag gtgtatttgg ttatccaaaa aaagaggcag ctaatcttgc
    88801 tgtaagtaga gttagactat ggctaaaaga aaatcctgaa aaaaatttaa aagttgtatt
    88861 taatgtgttt actgaagaag aagaagagaa atatagaaga atatttaaat aatattatgt
    88921 aacagaatcc taaaaata
//
LOCUS       DAMKPG010000005        74036 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMKPG010000005 DAMKPG010000000
VERSION     DAMKPG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 74036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.005"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            216..593
                     /locus_tag="PC621_000393"
     CDS             216..593
                     /locus_tag="PC621_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4107584.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            586..1620
                     /locus_tag="PC621_000394"
     CDS             586..1620
                     /locus_tag="PC621_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF4107585.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1686..2273
                     /locus_tag="PC621_000395"
     CDS             1686..2273
                     /locus_tag="PC621_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF4107586.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2651..3412)
                     /locus_tag="PC621_000396"
                     /pseudo
     CDS             complement(2651..3412)
                     /locus_tag="PC621_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4020..4397
                     /locus_tag="PC621_000397"
     CDS             4020..4397
                     /locus_tag="PC621_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4107587.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
     gene            4390..5424
                     /locus_tag="PC621_000398"
     CDS             4390..5424
                     /locus_tag="PC621_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF4107588.1"
                     /translation="MNNYMLYVFDNLIKTTIVCSFGICLLLFLKRYFFKRFSKRFNYY
                     IWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVSIDNDFMLYLVYLWLFVTI
                     AIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKELDIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIKFLVLSVKIMYWFNPLVYI
                     MSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNSNYLRNIGILEKTGADLKE
                     EDDISMRVVN"
     gene            5903..6076
                     /locus_tag="PC621_000399"
     CDS             5903..6076
                     /locus_tag="PC621_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF4107589.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(6161..7183)
                     /locus_tag="PC621_000400"
     CDS             complement(6161..7183)
                     /locus_tag="PC621_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF4107590.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(7514..7696)
                     /locus_tag="PC621_000401"
     CDS             complement(7514..7696)
                     /locus_tag="PC621_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107591.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            8009..8428
                     /locus_tag="PC621_000402"
     CDS             8009..8428
                     /locus_tag="PC621_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF4107592.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            8559..10400
                     /locus_tag="PC621_000403"
     CDS             8559..10400
                     /locus_tag="PC621_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4107593.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFVENKIGVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSIEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKN
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIETEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            10427..12313
                     /locus_tag="PC621_000404"
     CDS             10427..12313
                     /locus_tag="PC621_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4107594.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            12321..14321
                     /locus_tag="PC621_000405"
     CDS             12321..14321
                     /locus_tag="PC621_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107595.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            14583..15842
                     /locus_tag="PC621_000406"
     CDS             14583..15842
                     /locus_tag="PC621_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF4107596.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            15903..17690
                     /gene="iorA"
                     /locus_tag="PC621_000407"
     CDS             15903..17690
                     /gene="iorA"
                     /locus_tag="PC621_000407"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF4107597.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            17692..18267
                     /locus_tag="PC621_000408"
     CDS             17692..18267
                     /locus_tag="PC621_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF4107598.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            18320..19390
                     /gene="buk"
                     /locus_tag="PC621_000409"
     CDS             18320..19390
                     /gene="buk"
                     /locus_tag="PC621_000409"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF4107599.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            19608..20309
                     /locus_tag="PC621_000410"
     CDS             19608..20309
                     /locus_tag="PC621_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107600.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            20368..20874
                     /locus_tag="PC621_000411"
     CDS             20368..20874
                     /locus_tag="PC621_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF4107601.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            21049..22071
                     /locus_tag="PC621_000412"
     CDS             21049..22071
                     /locus_tag="PC621_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF4107602.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(22218..22526)
                     /locus_tag="PC621_000413"
     CDS             complement(22218..22526)
                     /locus_tag="PC621_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF4107603.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(22586..23014)
                     /locus_tag="PC621_000414"
     CDS             complement(22586..23014)
                     /locus_tag="PC621_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107604.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(23237..24694)
                     /locus_tag="PC621_000415"
     CDS             complement(23237..24694)
                     /locus_tag="PC621_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF4107605.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            25112..26026
                     /gene="nadA"
                     /locus_tag="PC621_000416"
     CDS             25112..26026
                     /gene="nadA"
                     /locus_tag="PC621_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF4107606.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            26039..27340
                     /locus_tag="PC621_000417"
     CDS             26039..27340
                     /locus_tag="PC621_000417"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF4107607.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            27321..28157
                     /gene="nadC"
                     /locus_tag="PC621_000418"
     CDS             27321..28157
                     /gene="nadC"
                     /locus_tag="PC621_000418"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF4107608.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            28413..28745
                     /locus_tag="PC621_000419"
     CDS             28413..28745
                     /locus_tag="PC621_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF4107609.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            28907..29092
                     /locus_tag="PC621_000420"
     CDS             28907..29092
                     /locus_tag="PC621_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107610.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            29119..30204
                     /locus_tag="PC621_000421"
     CDS             29119..30204
                     /locus_tag="PC621_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF4107611.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
     gene            30197..30655
                     /locus_tag="PC621_000422"
     CDS             30197..30655
                     /locus_tag="PC621_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107612.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            30690..31781
                     /locus_tag="PC621_000423"
     CDS             30690..31781
                     /locus_tag="PC621_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4107613.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            31786..32010
                     /locus_tag="PC621_000424"
     CDS             31786..32010
                     /locus_tag="PC621_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107614.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            32020..32583
                     /locus_tag="PC621_000425"
     CDS             32020..32583
                     /locus_tag="PC621_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107615.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            32544..33173
                     /locus_tag="PC621_000426"
     CDS             32544..33173
                     /locus_tag="PC621_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4107616.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            33213..33350
                     /locus_tag="PC621_000427"
     CDS             33213..33350
                     /locus_tag="PC621_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4107617.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            33350..34102
                     /locus_tag="PC621_000428"
     CDS             33350..34102
                     /locus_tag="PC621_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107618.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            34115..34600
                     /locus_tag="PC621_000429"
     CDS             34115..34600
                     /locus_tag="PC621_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107619.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            34607..34756
                     /locus_tag="PC621_000430"
     CDS             34607..34756
                     /locus_tag="PC621_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107620.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(34892..35713)
                     /gene="yidA"
                     /locus_tag="PC621_000431"
     CDS             complement(34892..35713)
                     /gene="yidA"
                     /locus_tag="PC621_000431"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF4107621.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            36205..37899
                     /locus_tag="PC621_000432"
     CDS             36205..37899
                     /locus_tag="PC621_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF4107622.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      38231..38341
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            38445..38816
                     /locus_tag="PC621_000433"
     CDS             38445..38816
                     /locus_tag="PC621_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF4107623.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            38912..39859
                     /gene="trxB"
                     /locus_tag="PC621_000434"
     CDS             38912..39859
                     /gene="trxB"
                     /locus_tag="PC621_000434"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF4107624.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            39944..40261
                     /locus_tag="PC621_000435"
     CDS             39944..40261
                     /locus_tag="PC621_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF4107625.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            40530..41816
                     /locus_tag="PC621_000436"
     CDS             40530..41816
                     /locus_tag="PC621_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF4107626.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            41928..42401
                     /locus_tag="PC621_000437"
     CDS             41928..42401
                     /locus_tag="PC621_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:42060..42062,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF4107627.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            42433..43743
                     /gene="grdB"
                     /locus_tag="PC621_000438"
     CDS             42433..43743
                     /gene="grdB"
                     /locus_tag="PC621_000438"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:43480..43482,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF4107628.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            44000..45532
                     /locus_tag="PC621_000439"
     CDS             44000..45532
                     /locus_tag="PC621_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF4107629.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            45543..46688
                     /locus_tag="PC621_000440"
     CDS             45543..46688
                     /locus_tag="PC621_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF4107630.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            46998..48077
                     /locus_tag="PC621_000441"
     CDS             46998..48077
                     /locus_tag="PC621_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4107631.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            48190..48789
                     /locus_tag="PC621_000442"
     CDS             48190..48789
                     /locus_tag="PC621_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107632.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(48976..49848)
                     /locus_tag="PC621_000443"
     CDS             complement(48976..49848)
                     /locus_tag="PC621_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4107633.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            50265..51668
                     /locus_tag="PC621_000444"
     CDS             50265..51668
                     /locus_tag="PC621_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107634.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            51695..53239
                     /locus_tag="PC621_000445"
     CDS             51695..53239
                     /locus_tag="PC621_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF4107635.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            53270..54661
                     /locus_tag="PC621_000446"
     CDS             53270..54661
                     /locus_tag="PC621_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF4107636.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            54854..56350
                     /gene="abfD"
                     /locus_tag="PC621_000447"
     CDS             54854..56350
                     /gene="abfD"
                     /locus_tag="PC621_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF4107637.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            56485..56760
                     /locus_tag="PC621_000448"
     CDS             56485..56760
                     /locus_tag="PC621_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107638.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            56784..58091
                     /locus_tag="PC621_000449"
     CDS             56784..58091
                     /locus_tag="PC621_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF4107639.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            58111..59229
                     /locus_tag="PC621_000450"
     CDS             58111..59229
                     /locus_tag="PC621_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF4107640.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            59516..60436
                     /locus_tag="PC621_000451"
     CDS             59516..60436
                     /locus_tag="PC621_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF4107641.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            60551..61708
                     /locus_tag="PC621_000452"
     CDS             60551..61708
                     /locus_tag="PC621_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF4107642.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(61793..63292)
                     /locus_tag="PC621_000453"
     CDS             complement(61793..63292)
                     /locus_tag="PC621_000453"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF4107643.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            63888..65312
                     /locus_tag="PC621_000454"
     CDS             63888..65312
                     /locus_tag="PC621_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF4107644.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            65347..67476
                     /locus_tag="PC621_000455"
     CDS             65347..67476
                     /locus_tag="PC621_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF4107645.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            67506..67928
                     /locus_tag="PC621_000456"
     CDS             67506..67928
                     /locus_tag="PC621_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4107646.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            67947..69098
                     /locus_tag="PC621_000457"
     CDS             67947..69098
                     /locus_tag="PC621_000457"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF4107647.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     regulatory      69204..69305
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            69488..70060
                     /locus_tag="PC621_000458"
     CDS             69488..70060
                     /locus_tag="PC621_000458"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF4107648.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            70124..70774
                     /gene="fsa"
                     /locus_tag="PC621_000459"
     CDS             70124..70774
                     /gene="fsa"
                     /locus_tag="PC621_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF4107649.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            70926..73850
                     /locus_tag="PC621_000460"
     CDS             70926..73850
                     /locus_tag="PC621_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107650.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT        29109 a         8000 c        13197 g        23730 t
ORIGIN      
        1 ttttaatgtg taaataacat tctacactat tgtaattaaa tatgtaaaaa gtaaaatatt
       61 taaatagata tattttaaaa tctaactttt aatattgtaa aaaaattaga aatttaacaa
      121 aaagtacaaa aattcacatt tttttcataa agtgtttgac taaaaaattc acatgttgta
      181 gaattaaggt agcaggagtt ttgagggagg tgtaaataat gataagaaaa ataccacaag
      241 cagaactaaa agtaatgaaa ttcatttgga aagtagatgt tacagtgaca tcaaaagatg
      301 ttattgaagc tatggaacaa aaatatggct ggaaacaaac tactacactg acacttttat
      361 ctaggctggt aaaaagagga ttcttagatg ctcaaaaaat agacagatat acacattaca
      421 caataagagt aaaacacaaa gattatttaa gttttgagac aaaggatttc ctaagtaata
      481 tacatgatga ctctttgaga agcttaattt cagcattaca tgatgatgaa aatataggta
      541 aagataagct agattttcta gaaagttatt ttgataactt aaaggatgaa taattatatg
      601 ctatatgttt ttgaaaatct tataaagaca actattgtat gtagtcttgg tatttgtcta
      661 ttattatttt tgaaaagata tttatttaag aagtttagta agagattcaa ttattatatt
      721 tggttgataa tagtctttag aatgttactt tttctattta attatacaat agtgtatgaa
      781 gtaaaagaac ccaaagaaaa tgcagtagga aataatatta cacaaattga tatttctaca
      841 gataataatc ttatgttgta tgtagcttat ttatggttag ttgtaactat agtcattgca
      901 gtttatacat ttataaagta tacaagattt aaaaatctag ttgttgatgt atcttatgat
      961 attgaagata atgatgtaaa ctgtatctac aaaaacttgt tgaaagaatt aaatataaaa
     1021 aagaaaattg aattaagagg ttctgatgaa ttgataagcc cagcaggcat gggattattt
     1081 aaatcttaca tattcttacc agactatcct tatagtaaag atgaactaac ttggattttg
     1141 aaacatgaat taatgcattt taaaaataaa gatattttaa taaagttttt agtgttatct
     1201 gttagaataa tatattggtt taatccactt gtttatgtaa tgagcaataa agtaaatcta
     1261 gattgtgaat tatgttgtga tgaaagtgtt ttgactgatt gttctttaaa ggataagaag
     1321 gagtatgcat tagcacttat aaagtcaatt aaacttagta agaattataa tagtggtatt
     1381 ttaacaacag agtttaataa gacaagtctg gaaaagagac ttgacagtat tgttaagaaa
     1441 aaaggtaaaa atggaatatt ggtgttattg attttgatta cattattttc agttacatat
     1501 tttaattttg aaagtattag ttttgataat tcaaattctt taaggaatat ggggatttta
     1561 caaaaatcag gagtagatct taaggaagaa gatgatatta gcatgagagt tataaattaa
     1621 tagattaatg tatgtaatga aaaggtatat tgtcgtatta ttaataaatt aggaggaatt
     1681 atgaaatgaa aaatttaaaa aaaattttaa taattacagg ttgtctaact ctaatatcta
     1741 ctagtttggt atttgcaaat actactactg atgtagatac atcaaaggat gatgattcaa
     1801 taataagttc atgtactata actggttatg aatttacata tgaaaacgca ccacaatctg
     1861 taaaatttga ttatgaaaaa aaatgtgaag cgctaaatat aaaaccatct aaaaatgata
     1921 taatttttgt taacaaagat tctatagatg caaatgatgt agaaaactat gttgagccaa
     1981 aagcatatat tattagatat aaaaaaggtt atgtttttga agttactgga cctaagagtt
     2041 atacattaaa tgtaagtaat actgtagtcg gatataatcg tgttgaatca ggtaatcctg
     2101 tgcatttagt tcaactttta tgtagaacct tagatggttc aagtattgat gatgatagtg
     2161 aatttggacc tgctacatat aatgcagtta agtcacttca aggaagactt ggattaacaa
     2221 gagatggaat tgttggaaga agtacttggg atgcagctgg aagacgtctt taaaaagaaa
     2281 gctattgcac agataatttt aaattgctga atctgtaaac atttacatgt attctttaaa
     2341 taaaaattta ttttttaagt gataattaaa tttttactaa tagggctatg gctttaaaaa
     2401 tcatagcctt attttgatat cttttccatt tttgcaaaag ctattaaccc tttaagaaaa
     2461 tttcttgttt ttttgaaaaa tttctttctt caaaataaat acttaaatga gctaaaattt
     2521 gagcacaatt tctaattttc tgagtccatt tttaagaaat atatatggtt gttaagtata
     2581 gtgatttgtg caaataatta tccaaagtaa aaatagattt caatttagta acttttctta
     2641 ataatctatt ctaacttccc attgaatttg tagtatatat cgatgtattt atttcttgag
     2701 agtacttaaa atatgttact aattcattcc aattgtttca ccaacctctt aatactattg
     2761 tatattgatt gtactatttt ttctctagtg atagctcact taatgctacc tcttctgtaa
     2821 ttgtagcata tctttcttta aatcttttac agattctttt ctatttttat atgatacata
     2881 atttaaagta tttttaattt gatgcacaac atatctttgt acttaagtat caggaaatat
     2941 cgctttaatt gcattagaaa attcaactag tctatctata gaagctgcta ttaatacatc
     3001 tttaattccc atgtttctag catctataag cacttttagc caatactttg aaatttcatt
     3061 ttctccaatc catattctta aaacctcttt tatgctacat atagctatat agactgtttt
     3121 gtttattatt cgacctcact tctaacttta taatgtatag catctataaa tacaattgaa
     3181 taaattatat taagaggtct aagttaccat ttatgagtta aaggaataac tttatcagta
     3241 gttttagtta ttagtgaatg agataatatc aattccataa gtacttttaa gatgacttaa
     3301 tatattttga gtagacatgc cttttgagta tattccaatc acttgatttt ttattcctaa
     3361 tatatcattt tctatcataa cctaaaagtt cattaagctc cgcttctaac atttaatgga
     3421 ttgtactttc aaataaatct tttagtatat cttgaatatc tgtaattgtt ttggcatcat
     3481 attcttcaat aaattttttt aatattttag tttttgttaa acataaaaag tgaacatctt
     3541 cctttgtata gtagtgatat tttaattatc acaacttttt agaagaatac aaactttatt
     3601 ttaaagacac tgaaaatggt gtttttatac ctttatttgc tatgtataat tttacttata
     3661 acataaactt atataaaaca gatattttaa taacacaaag gtacaattag gagattgtta
     3721 aaatttatta gatattactc tagtttattt aaaaaaaagt attataatat tccttgtgtg
     3781 aaaatatagg aatttttatt aaataatttt aatgtggaaa taacattctg cactattata
     3841 attaaatatg taaaaagcaa aatacttgaa caactatatt ttaaaatcta acttttagta
     3901 ttttaaaaaa ttagaaaatt aataaaaagt acaaaaatcc acattttttc ataaagcgtt
     3961 tgactaaaaa attcacatat tgtagactta aagtagcaag agttttgagg gaggtgtaaa
     4021 tgatgataag aaaaatacca caagcagaac taaaagtaat gaaatttatt tggaaagtag
     4081 atgctacagt gacatcaaaa gatgttattg aagttatgga acaaaaatat ggttggaaac
     4141 aaaccactac actaacactt ttatcgaggt tagtaaaaag aggattctta aatgctcaaa
     4201 aaatagacag atatacacat tacacagtaa tagtaaaaca caaagattat ttaagttttg
     4261 agacaaagga tttcctaagt aatatacatg atgattcttt gaaaagctta atttcagcat
     4321 tacatgatga tgaaaatata ggtaaagaca cgctagattt tttagaaagt tattttgata
     4381 atttaaagaa tgaataatta tatgctatat gtttttgaca atcttataaa gacaactatt
     4441 gtatgtagtt ttggtatttg tctattatta tttttgaaaa gatatttttt taagaggttt
     4501 agtaagagat ttaattatta tatttggttg gtaatagtct ttagaatgtt actttttcta
     4561 tttaattata cagtaatgta tgaagtaaaa gagcccaagg aaaatacagt aggaaataat
     4621 attacacaaa ttgatgtttc tatagataat gattttatgc tgtatttagt ttatttatgg
     4681 ttatttgtaa ctatagccat tgcagtttat acatttatgg aatacacaaa atttaaaaat
     4741 ctcgttgttg atgtatctta tgatattgaa gatcatgatg taaattgtat ctataaaaat
     4801 ttgttaaaag aattagacat aaaaaagaaa attgagttaa gaggttctga tgaattgata
     4861 agtccagcag gtatgggatt atttaaatct tacatattct taccagacta tacttatagt
     4921 aaagatgagt taacttggat tttgaaacat gaattaatgc attttaaaaa caaagatatt
     4981 ttaataaagt ttttagtttt gtctgttaaa ataatgtatt ggtttaatcc acttgtttat
     5041 ataatgagca ataaagtaaa tctagattgt gaattatgtt gtgatgaaag tgttttgact
     5101 gattgctctt taaaagataa gaaagcatat gcgttagcac ttataaagtc aattaaactt
     5161 agtaagaatt ataatagtgg tattttaaca actgaattta ataagacaaa tttggaaaag
     5221 agacttgata gtattattaa gaaaaaaggg aaaagtggaa tattgatgtt attgattttg
     5281 tttacattat tttcagttac atattttaat tttgaaagta ttagttttga taattcaaat
     5341 tatttaagaa atatagggat tttagaaaaa acaggcgcag atcttaagga agaagatgat
     5401 attagcatga gagttgtaaa ttaatagatt tttatagtat tataatttat taacaaatta
     5461 ggagaagttt tgaaattaaa aatctaaaaa aaatcttagt agtcacaggg agggtgactc
     5521 taatatctat tagtttagta tttgcaaata ctaataatga tgtaaatgta tgaaaaagta
     5581 atgaagagca aacaacaaat tcatttacaa taactggtta cgaatttatt tatgaaaatg
     5641 taccacaacc tataaaatct gattatgaaa aaaatgtaaa gaattaaata taaatccatc
     5701 taaagatgat acaatcttta ttaatgaaaa atttatagat tcatataata ttaatataga
     5761 tgattataat attgaaccaa aagcatttat tattagatat aaaaaagatt taaatcgttt
     5821 tgatgctact ggtcctataa gttatagcgt aaatataaat accactgtag taggatatgg
     5881 acatgtagaa aaagggaatc ctgtgcattt agtacaactt ttatgtcaac aaataaatgg
     5941 ttctgatatt gatccagata gtaaatttgg acctgctaca tataatcaag taaaaatact
     6001 tcaaggaaga cttggtgtaa caaaagatgg tgttgttgga aaagccactt ggcaagctgt
     6061 cggagagcgt tattagaaaa gaggttgtcg cactaagaaa tagggcgaca acttttttat
     6121 atagaatata ttaatcagta tcttagagtc tagaaatatt ttacaataaa ttccatataa
     6181 ctctaccatc tatatcctta acattttgac aaccagtcaa aatcatagtc tcacataact
     6241 cattcctaac cttctctata aaaaactcca caccatcaag accaccgcca aaagaagcag
     6301 taacaaaagg tcttcccatc aaaacagcat cagcacccaa tccaagcatc ttaactacat
     6361 caacaccagt cctaactcca ccatcaacaa gtattgttat ctttcccttt acagacttag
     6421 ctatatctgg aagtacctca caagtaccag gagtataatc aagtactctt ccaccatgat
     6481 ttgaaacaat aatggcacta gcaccagact ctacagccat caaagcatca tctacagtca
     6541 taattccttt tataataaaa ggtaatttag tagattcaac caattctcta atctcatcaa
     6601 ttgtcttagg agaaaaagga ttttcttgaa attgattatt aataagtcca caagcatcaa
     6661 ggtctacacc aactgcaaaa gcacctgctt cttcagacaa tctaattttt tctattatct
     6721 ttgaattatt ccaaggcttt ataaatacaa ttccatttcc actattatct ttcaacacat
     6781 ctaaattatc cagtaaaaaa gtatctacat tagtatcgcc aaccatagca taaattccag
     6841 aattagaaca acctctaaca acaggctcta tgtattcttt ttcggaaact ttaccaccca
     6901 tgttcaatag agtacctgat acaggtgcag cgaatatagg agaactcatt tttctaccaa
     6961 ataattctat ggatgtatct ggctttgaaa cattatgtat aactctcata tttatcttta
     7021 ttttttctag actttttcta ttttcaatga aagacgaacc acttccttta ccacccatac
     7081 caggaacatc tccagcacaa gctaatccat tacaaatttt acatacttta catgttccat
     7141 taaagttctc tctagctgat tttagtaagt cattgtaatt catacaaaat ctctcctttt
     7201 tataatatta aaattgagta ttactatatt gaaaatatct taattgtaaa tatagggatg
     7261 ttattatgat gagctaaact agataataca tattagatat tttttattgt tgtagatagt
     7321 taaattatat catattagat agtcaaattt tgaaaaaaaa ctaacttatt taagtataca
     7381 ttttgaaaaa attaaaaaag ctatatatag atttttaaag aaatctatat atagctttta
     7441 agtttatgtc aatctttttt tattaatgaa taaataatat agaatacaat atacaaaaaa
     7501 gtttcttaaa atgctaagat aatccccatt tacaaagcaa catcattata ctgtgtaaat
     7561 ttgaatcaac aatgttttca ccatatttca ccataaaatc ttctaattgt gtgtcaataa
     7621 atgaataata tccacttata ctatcgatgt atgcagtcat taagtgtata cattcatcac
     7681 ttttatcttt atacatatat atctccccct atatacaata tataaattta gatactaaat
     7741 cgtatcatat taaatagtta agtgtgtaac atctatgcag ttatatattt atatccttat
     7801 attaataact aaagtgagtt ttgacaatag attttgggaa actggatgtt caaagtgtaa
     7861 acttggattt taaaatcatt aattaagata gtgtatagga atatatgtat acataactta
     7921 attataaaga ttataaacaa ttacttttat taactttatt taagtggtag aataacttgg
     7981 agattaataa ttataaggag gatataatat gagtttatac aagtttatat atgatgataa
     8041 ggaatattta ttaaaagagg agaactgtag tgctttaata aatgatgaag aaaatccagt
     8101 tcaaggagta agtatttcaa aaataattga tatacttaat gaagcagaag aagttgattt
     8161 tgatgtagaa tattatcaag aagcatgtcc attatgttta gaaggagtaa aagaaaagaa
     8221 aaaattcttt ccttttttag aatatcactt ttatattttc agtaaagatg gagaatatgt
     8281 aataagcaat atatctgttg attataaagg actatctttt aataaactat caagggcaaa
     8341 taaagttgat aatagttata ttgtaagtgt gataatttgt gaaaattgtc aggattatat
     8401 agttcagata gaaaattgta tagtttagtg taaagtatgt cttaatttaa gagaaatttc
     8461 taaattgaga catcaatttt tttcaaaaat agtatggaaa attactaaat ttatgagata
     8521 atgtaagtcg gaggtgagtt acaatatata gtaaatattt ggaagagata gatgatacaa
     8581 ttttagattc aatgtttaaa ctaaccttga atgaaagatt attgactaat aacgattttg
     8641 ttgaaaataa aattggtgta gtaaagaatt taattgattg ctataaaact gaacaaaata
     8701 gtttaatgtt attaaaatca aattttttgc tagctttatt atatggtatt caagggttta
     8761 gtgaaaaaat gaaagagtat attttgattt cctgtaaata tattgataaa tgcttaccca
     8821 aagattataa atttttagca cgattttatt cacaaatagc tattttaagt ttaaaaaatg
     8881 aagatgtaaa taaggcaaat ttatatatgg ataaatttaa tcttatttgt gatgaaaata
     8941 attttctaat tgaagaaatt atttttaaat cacaacttat ttacattaag gcaagtaagt
     9001 gtatagaatc ttctgtaata attaaagaaa tagaaacatt gtatataaag ataaaagaaa
     9061 ttaacgattt taactgtaaa aaaatatatt tttttattat tggtaaaata tattttttgt
     9121 ttttggatga tgctctaatt gctaaagtaa attttttaaa agcaagaaaa tatgcagatt
     9181 tactaaagga tatgcaagta tcttcactat gtaatataaa gataggtgag tgtgaatgtt
     9241 catttgaaaa ttatgaatgt tccatagaat atttcaatga agtaataagc aataaaaagt
     9301 atagaaatgt aaatataatt caaaaatata gagcatcaaa caacataact aaaatattaa
     9361 taaagactag aaattatcct gtagcaataa ataatttagc aaaatctgaa gtatatctag
     9421 aaaaaattaa aaatcagagc ttaaaagaag tagagaagtt tgatttattt ataagtttag
     9481 ctatgtatta tgcaaaaagt aatgaaaaat cttttgaaca atcaatatgc tatttagata
     9541 gagctaaaaa catcttaaat atgataaaaa atatagaaga atatacaata tatgatttag
     9601 aaataattta tcatcaggca aagatatatt atatctttgg atattatgac aatgccttaa
     9661 acacaaataa aaaactcctt gaaaaatcta aaaatgctaa aaattataat tatgtgaagt
     9721 tagcatacaa gaatatatat ttgtgttttg aaaagattca aaattatgaa atgagtatga
     9781 aatactttaa aatgtattat gagttaaaaa tgatgtatat aaaagctcga aatagaaatt
     9841 atatagattc cttaaattat agacatgagt atatagaaac agaaataaat aacatgagaa
     9901 aagtaaaact tgatttagat aaaaaaaaat atgttgacca tttgacaagt gcatataata
     9961 gaactttttt gaacgatttt ttagaaaaac aagaagttag tgaatattgc acagcattta
    10021 tgatagatgt agattacttt aaagaataca atgatactta tggacactat aatggagata
    10081 ttgctcttaa aaatataact ataataataa aaaaatatct aaaaaaagac atgtttatga
    10141 taaggtatgg tggagaagaa tttctgatat tatctgtatg taaagattat aaaaaaagca
    10201 aaatatttgg aagaaaatta tgtaggattg taaagaaatt tttaaacaat aatttgacag
    10261 ttagtattgg cattgataca tgtaaaaata gtagtataga tataagagaa ataattgaaa
    10321 atgctgataa agctttatat aaagcaaagg aaagtggtag aaatagatgt ttgcattatc
    10381 atgattttaa gaatttttaa ctgttgaaat aggattggtg atttacttga aaaggagttt
    10441 aatagaagat ttaaagagaa atataaacaa gatagaatac gatatgttga ctaaattcta
    10501 tctaaatttg acagatttaa actacatctt ggattatcac aaagaccttt taaatacaaa
    10561 actagaagat aatagtagag tctatacttt atatttaatt agccttataa actatataaa
    10621 aaaagataga gaagcatcat acaactattt gatagagtca ttaaagtata ttaaaaaatt
    10681 gtcattaaaa gaagattcag atataatatc taaaatttat atttatctta ctttatcagc
    10741 tatttctata agaaaatatg ataaggcaaa taatttttat ttctcagcaa aaaaagttat
    10801 aagacaaaat catttaaatg agttgctagt cttgttaaat atgagtttat gtgtagaaat
    10861 aagtgcttta tatcagacca aaacggatgt aatagcttta gtagaagaag tgtctttttt
    10921 taaaacttta aaaaataaag ctcttgtaag tagggcaaat tatatatttg ggagagttta
    10981 tctatattta tttaataact ttataaaaag tatggagtac ctaattgaat gtctaaaact
    11041 ggctcaagaa aataatttat attctataga ggctttttgt aaatgtataa taagtttgtg
    11101 ttatttagag agtggaaata atgtagaagc tataaaatac ataaataatg tattagatgt
    11161 tgcgcacagt aagaactcaa tatcagtaac tgaaagagtg tctataaaga tagaccttat
    11221 atgggcatat ttaaaagaag atatgaataa agaagcagaa gttattttat taaagactat
    11281 aaaattaatg aaagttgttg aaggtgtata taaggattat ttatattcat ttatattttt
    11341 atattcagct gaaattgagt taaggaaaga taattgtagt tttgaaaagg taaataccta
    11401 tttaatttta tcaaaaaata tatataaaag ttttggcgat gtttttatat ataattctaa
    11461 actagattac atatataagt tgtatggaga tttatatata aaatttggta atatagatga
    11521 aggtataagt tgttatataa aaggtttaaa tttagttaaa aagagttcat tgaatcttag
    11581 aaaaatatct atattttatg ggctaatagc aaatggatat gagctaaaaa aagattttaa
    11641 actggcaata gaatattata aaagtatgga ttggtattct gataggtggg aaaaaaataa
    11701 ttcctataaa acatctcaag caattcacaa acaatatgaa ctcagacaaa aacaagagag
    11761 tttaagatta ctagtggatg ataataagaa attagaaaat gatattttaa aagatggact
    11821 tactaaacta tataatagaa gatatttgga acagcagttg gatttattta atggaaccag
    11881 agacaaaaat atggttgcaa tactagtcga tatagattgt tttaaaagtt ataatgataa
    11941 ttatggtcat ctagcaggag ataaagttat agttaaggta actgatatta taagaagtgt
    12001 atttatagat attaccgaac atataatcag atatggggga gaagaaattt taatactagt
    12061 agattctata gaatcaatcg gtgttgaaga gattgataat atagaattat atattaaaag
    12121 agttttcaaa cttcttgaat ttgagggtat tgaacatata tattctaaaa taaaaaacta
    12181 tataactata agtgctggag taagtataaa aagatgtcgt agtagaaaag atgttgaaat
    12241 attaattgaa gatgcagata agaacctata taaatcaaaa aaagcaggta gaaatcaata
    12301 cattataatt taaaattgag gtgattttgt tgcgaaaaaa aataggaatt atagcctctg
    12361 atattgaatt aaaagaaaga atagaagaat tatatagaga agatgtagaa aatggaacta
    12421 taataatcga catacttaac ctagacttaa tggaaaatca aggtcgtata cttgttgaaa
    12481 aaggtgccca agctataatt ggtagaggtg gaggttatag tttagtaata gatacagtaa
    12541 atgtacctgt tataccaatg aatatgaagt caactgacct tttaagagca atagaaatag
    12601 caaagaaata ttctaaaaaa gtagtattga ttcttggaga caatgaagtt tcttttgact
    12661 atgttggctg gagaaatgtc ataagtactg aaataactga agaatggttt gaatctaagt
    12721 atgagataag aagtaaagtt gtaaaatata tagaccaaaa agatgaagtt gtaatcgtag
    12781 gaggaggttt ggcatgttct tttgcaaggc aatatggaat tgatagtgta tttgcaactg
    12841 ctagtgatga atctataaga gaagctgtag aatattgtaa aaaattattg gatacattag
    12901 gtgaagaaaa gtttaataat gaagtgttac gaaatatact tgatggtata aaagatggag
    12961 taattgctat agatagtaat ggtagtataa ttttgtacaa tgaaagtgca aaaaacatgc
    13021 ttaaagtaga gagaaaatgt gcacttaata aatacatctt agatgtattt cctaaaatgg
    13081 agtggatgtt agattgttta catgaaaaag aggatgtaga agatagaaag ataagaaata
    13141 taaataatct aattgttaat actaggacaa cattaataaa agttgataat agtacttatg
    13201 gagttttagg aataatacaa gatataacca agctacaaaa cttagaaaga aaaataagat
    13261 ttgacttgaa tcaaaaaggt ctctatgcta gatatacttt tgatgatttt ttatttaaag
    13321 ataaattaac tagagaattt attgaagaag ctaaaaaaat tggaaaaagt gattatacaa
    13381 ctttacttta tggagaaagt gggtctggta aagaaattat agctcatagt atacacaata
    13441 taagtaaaag aaaagatagg ccttttgttg ctataaattg tgctactata gcagaaaatt
    13501 tacttgaaag tgaacttttt ggctatgaag aaggtgcatt tactggtgct agaaagggtg
    13561 gaaaaagagg actatttgaa cttgctcatg gagggactct ttttttagat gaaataaata
    13621 gtctatcctt taatattcag acaaaactat taagagtaat agaggaacgt caaataatga
    13681 gaattggttc tgattatata atacctctgg atataaggat tatagcagca acgaacgaat
    13741 ctttaaccga aaaaatagtt atgggtacat ttagagcaga cttattttat agattgagta
    13801 gtttagagat aaatatacct cctttaagag acagaagaga agatataata cctttgttta
    13861 acaattttgt aaatgaagta ttaaaggatg atggtctcaa tggaataaac agtatagatg
    13921 aaaattttgt tttgactaaa gatgaaatgg ataagctata taattatagc tggcctggaa
    13981 atgtaagaga gttaaagaca atagctcaaa aatatgttgt gacaggtaaa attaaattga
    14041 gacaagatag agattttaaa actaaaaaat tacttcctaa ctctgaagta gacaaattta
    14101 attctgaaac gacagcaagt gtagaagtcc aagatgaatc tataaatata agtaagataa
    14161 atgatggtaa gataagtata gatataaaag aagtaaataa gtatgttgaa gaaaaaataa
    14221 taagtatgct ttttgctcag ggactttcta aaaatgaagt tgcacaagtt cttggaataa
    14281 gcaggacatc tttatggaag aaatacaata aaaatattta aatataatta catataaatt
    14341 attttgaagt aatgttaatt ttttaaaaca agtgttaaga aaaattaaca ttactttttt
    14401 gtaaattttt atttttttag acaggcatat atatttataa gaaaaatagt taaaacactt
    14461 aatttaataa tgaaaaaaat aataattcta aacaaaatca attttacata aagtgggatt
    14521 tattggcata gtaattgctt aatatataat gtaagagtaa atacaatttt aggggtgatg
    14581 gtatgagtca aagtatggtt ggtaagcatg caatgtggcc aaaagaaaat gatgttatat
    14641 ttagcatatc tggtagagct caagcagcag agaaggcttt tggtatggat aatgtaataa
    14701 atgcaacaat aggcgctcta atggatgatt caggaaaatt aataacaatg aaaactgtat
    14761 atgaagaata taaagcatta gataactgtg agataggtgc atatgcagct cttgaaggtc
    14821 aaccagacta cttagaagca gttaaaaaag tatttttcag agattactta ccagaaggac
    14881 atataagagt attagcttca ccaggaggaa gtggtgcaat aaaattagct gtatggaact
    14941 atactaatga aggagatgag gtattaacat ctgattggtt ttggagtcca tatgtaagta
    15001 tagctgaaga ggctaataga aaagtagtta attaccaact ttttgatgaa aatagaaggt
    15061 ttaattttga gtcgttcaaa gaaaaatttg taaatatagc agaaaaacaa ggtagagtct
    15121 ttacaattat aaacacacca gcgcataatc caactggata tagtgttgca gatgatgagt
    15181 gggataaaat attagactta tcaaaagaag tcgcaaaaga taaagacaaa aagataatat
    15241 ttttcgtaga ttcagcatat attgactttg caggtgatga tgatgtatgt agaaagtttt
    15301 ttaagaaatt ttctaatttg cctgaaaacg ttctcgtatt ggttggattc agtatgtcaa
    15361 aaggatttac agcttatggt atgagaatgg gggctataat ttgtatatct tcaagtgaag
    15421 atgtagctga agagttccat tactcatgtg ttcattcatg tagagcaaat tggtcaaatt
    15481 gtaatagaag tgccatggca gtattatcta acattgtaaa tgaccctaaa aaattcaaag
    15541 aatatgagga tgaaaaagaa atatacaaaa acatgcttac tagaagagca gatgtatttg
    15601 tagaagaagc tgaaagagta ggattagaaa ttcttccata tatagcaggt ttctttgtaa
    15661 gtataccatg tgataatcca aaagaagtat gtgaagaatt gactaaacat aatttatttg
    15721 cagttccatt aaaaatgggc ttaagatttg cagtttgtgc agtttctgaa gataaatgta
    15781 agaaagcacc aagtattata aaagaagcac ttgaaagttt agaagtaaaa ataaataatt
    15841 aatttcattg aacaaattgg gtggactatg aatttaataa ttgtagatgg agggaatttt
    15901 atatgaaaca attgatgaca ggtaatgaag caattgcacg tggagcctat gaggctggtg
    15961 ttaaatatgc atctgcatat ccagggactc caagtacaga aatattagaa aatatagcaa
    16021 catataaaga tgcaatagtt gcagagtggg ctccaaacga aaaggtagca cttgaggctg
    16081 ctataggggg ttcaattgca ggagcaagaa caatggcttc tatgaaacat gttggtttga
    16141 atgttgcagc tgatccaata tttacttatg catatacagg ggtaaatggt ggtatggtac
    16201 ttattacagc agatgagccg ggaatgcatt catctcaaaa tgagcaagat aataggatgt
    16261 atgcaaaatt tgcaaagata cctttatttg aaccatcaac tagtcaagaa gctaaagata
    16321 tgataaaaga agcttttgaa gttagtgaaa aatacgatac accagtttta tatagagtta
    16381 caacaagact ttgtcattca aaaggattag tggagtgcta tgatagagaa gaagttgaaa
    16441 ttaaagagta tgttaaaaat gctaagaaaa tggtaactgt tccagcaaat gctcaaataa
    16501 gaaggggcgt tgttgaagaa agaatggaaa tccttaaaaa attctcaaat gaaactgatt
    16561 taaactacta tgaaatgaat gatacaaaaa taggtgttat agcttctggt atgtgttgta
    16621 attttgccaa agaagttttt ggaaaaaatg catcatatat gaaactagga tttacaaatc
    16681 cattacctta tgaaaaaata aaagaatttg cagaaaaagt tgataaaata tatgttatag
    16741 aagaaaatga cccatttata gaagaacaaa taaaagcata tggaatagat tgtattggaa
    16801 aagatgtaat accaccatat ggagaaatga caccagatgt tttaagaaaa gctatatttg
    16861 gaaaaacaaa tgatactata gaatacaaat ctgaattagt aacaccaaga ccaccaagtt
    16921 tctgtgcagg atgtccacat agaggattct tctatgaact tggaaagaga aaaaatttaa
    16981 tagttggtgg agatataggt tgttatactc ttggatttgc tcctccatat aatggaatag
    17041 attatgttgt ttgtatgggt tctgcttttg gaactgcaca tggagctcaa aaagttctta
    17101 atatgaagga tgataatgaa aaaagattgg taggtgtact tggagactca acattcttcc
    17161 atacaggaat aaatggactt ttagatgttg tttacaatag aggtaattct atttcggtta
    17221 tattagataa tagaataact ggaatgacag gtcaccaaga aaacccaggt tcagggtata
    17281 ctttacaagg tgctaaaact aaagaagttg atatagaagg attagttaaa gcttgtggaa
    17341 tagaacatgt aagagttata aaccctaaca atttaaaaga agtaaatgaa gcactagatt
    17401 gggctttagc tatagaagat gaaccatcag taataataac aagatggcca tgtgtactta
    17461 aaaaattctc taaagaagat atagaagaat ttaataatcc atttaagact aagtgtaaag
    17521 ttgaccatga taaatgtata ggatgtaaat tatgtttgaa aactggatgt ccagctctat
    17581 catttgataa agagaataag ttatcaaata tagatagaaa tcagtgtgta ggatgtggag
    17641 tatgtgcaca agtttgtcca aaacaagcaa tagttaagga ggaaaaataa tatgactaag
    17701 agtatacttt tggtaggtgt aggaggtcaa ggaactatac ttgctagtaa attattaact
    17761 atgggattga tggaagctgg atatgatgta aagatgagtg aaatacatgg aatgagtcaa
    17821 agaggaggtt cagtttcctc acaagttaga tatggagatt gtgttcattc tccagttata
    17881 gaaattggtg gagcagatat attagtatct tttgaaaaaa tggaagcttt aagatggttc
    17941 aattatttaa aaccagaagg aaaagcttta gtaaataatc ataggataga ttctatgact
    18001 gttttaatcg gaggagctga atatcaagaa agtgatatag atgcagagtt aagtagatta
    18061 aatgcaaaag ttataaatgc agctgataaa gctgaagaac taggaaattc aaaaataatg
    18121 aatgttattt tattaggatg tctagttaaa tctatgcaat tagagtctat tgattgggaa
    18181 aaaattataa gtgaaaatgt taaaccaaaa tttgttgaat taaacataaa agctttccat
    18241 gaaggtatgg aaatggttag caaatagttt ataatagata taaatagact atagaaatta
    18301 aggtgaaagg aagaaaatta tgacttacag aatattagcc ataaatccag gttctacttc
    18361 tacaaaaata gcagtatatg atggagaaga acaaattctt gtgaagacga tagaccatcc
    18421 agctgaagag attgcaaaat ataatactat acaagaccag tttgaaatgc gtaaggaagc
    18481 agttttgaat attcttaaag aaaatagtat agacttaaaa tctcttagtg caatagtagg
    18541 aagaggtgga gttttaccac cagtaaaatc aggagcatat ctagtaaatg aagaaatgat
    18601 tgatgtacta agacatagac cagtacttga acacgcttcc aatttaggtg ctgttgtggc
    18661 acatgcaata tcagaacctc ttggaatcaa ctcatatatt tatgattctg ttgcagtaga
    18721 tgagcttata gatgtagcga gaatatctgg actttgtgga atggatagat caagtgcagg
    18781 gcatgcatta aatactagag caatggcttt aaaatatgct aaggataaag gaaaagatta
    18841 taagagctta aacttaatag tagctcacat tggtggagga gtaagtattt atcttcatga
    18901 aaaaggaaga atggttgata tgctatctga tgatgaagga ccattttctc cagaaaggtc
    18961 aggaagagta cctgctacaa aattagtggc tgcctgttat tcaggtcaat attcagaaag
    19021 agaaatgact aaaaagataa gaggtaaagg tggtatagtt tcatacctaa atactgtaga
    19081 tgctagagaa gttgaaaaaa tgatagcaga aggaaatgaa gaagcaaaaa ttatttatga
    19141 agcaatggct tatcagttag caaaaggtat tggagagtta gcaactgtag tagatggaaa
    19201 ggtagatgct ataattataa caggtggaat tgcatattct gaaatgttta cttcaatggt
    19261 taaaaagaaa gttgagttta tagcaccagt agaaattatg gcaggagaaa atgagttgga
    19321 atcacttgct tttggaactt taagagtact aaatggagaa gaagaagcta gaatttatag
    19381 tgaaaattaa taagaaaaat taaaaatgtt tatatactaa aaaagtggta tatccgtttt
    19441 ttatacacga aagcttagag tataggtgaa aatgtataaa atttggattg ccacttttta
    19501 taattaaaat tttatcttaa tctaataaat aattctgtaa aatttcgtat ttatatatat
    19561 attttattgg tatttattgt tattattatt atgaggagtg atttttgatg ggaaaaataa
    19621 gtttaaaaag tatgataaga ttattttcag gtttttttat atttgcaatt tcttctgtgc
    19681 ttatgattaa tgcacatgta ggtcttatgc cttgggatgt acttcaccaa ggtttatcta
    19741 taaagctagg cataactata ggtcaggctt ctataatggt aggagttgta attgttatat
    19801 tggatgcagt ttttggtgaa aatataggat ggggtacttt actcaacatg acatttatag
    19861 gaatttttat tgatttagta atattttctg gagtgatacc tcatgcaagc aacacttata
    19921 taggtgtgtt tatggttgtt ataggaatta ttttggcagc tatagcaagt tttctatact
    19981 taggtgtttg tttaggaagt ggaccgagag atgggcttat gattgctctt cagaaaaaaa
    20041 ctaataaatc tgtacgtctt gttagaacaa ttcttgaaat actagcatta gttgttgggt
    20101 ggttattagg aggttctgta ggtattggta cgcttgtaag tgctttaggt ttaggatatg
    20161 tattacaaat agtgtttaga atatttaaat ttgacacaaa gttgctgaaa catagattta
    20221 ttatagatga tataagagaa tggaaggaaa agagaagcaa tgaacataaa tgtaaatcaa
    20281 gtattgtaat aaaaaatgag cagaattaat tatgctaaaa tatatttgta gtttatctaa
    20341 tttatagtaa agtcaggaga ttttgaaatg gaattatggg atttatataa tgctgatgga
    20401 attaaaacag gaaatgttat aaaaagaggt aattctattg aagaaggtta ttaccatttg
    20461 gcagttgaag tgtggatttt aaatagtaac tctcaaattt taattcagaa aaggtctaaa
    20521 agtaaaaaga cattgccaaa tatgtgggga atgacaactg gatgtattgt ttctggggaa
    20581 gatagtttag aaggagcaat tcgcgaagca aaggaagaaa tagggataga tataactaaa
    20641 gatgagatga aagtttttag aagtatgatt catgaagata ctctttggga tgtatactta
    20701 gttaaaaaag aatatgatat atccaaagct attttacagg aggaagaagt aagtgatata
    20761 aagtgggtaa gcacagatga aataagacaa ttactaaaag agggtttgtt ttttgaatat
    20821 ccagagatgt acgaattgct atatgagata gataataata aattaaatat atgatgtaga
    20881 gcagatttca gtataagtaa gattatttaa aatgtatctt ctctaaattt taatattgtt
    20941 ttatgaaaat taataagtta tttttagtat ttttctactt gttttttgag attaaaataa
    21001 aaagggcagt cattataaag tcataaaaat ttggggagga tttaaactat ggaagataac
    21061 aaaggattgg cacgtgatag ttcaaaatat aaaattgttt ctatatctgt cgtagtaatt
    21121 attctaatta cattttggta tatgttaaat atggtgcttt taacatttat tatgactttt
    21181 attttctata acttgcttgt agccacaaga aaacgaatta agaagttttc ttctttaaat
    21241 attccagatt cattgataat tatagtgtta tatgcactat ttgctatatt gttagtattg
    21301 attagctatg cagttgtacc aataattata gttcaactta cagagctgag tagggttttt
    21361 agtgattttg atgtaaatca atttgctcaa tctttaggac caaaattgta tcctattgtc
    21421 tcaaagctgg attttaataa atatatttca caagcagggc tacttatagc atcaacagca
    21481 acaaaagttg gaagttttgg agtaaatata ttattggcat ttttactgag tttactcctt
    21541 ttacttgaaa aaaatgaaat taaaaatttt ggagataaac tttctgatag taagatttct
    21601 tttatttata attcgcttgt attttttgga aagagctttg ttaaaaattt tggtgaagta
    21661 atgaaagtac aagttatgat tgcctttata aattctgttg tttctatgat tttcttaggt
    21721 ttcatgggat tccctcaaat atgggcttta ggatttatga tttttgtgtt gggtcttatt
    21781 ccagtagcag gagttatagt gtctttgatt ccattaactg taatagcatt taatacagga
    21841 ggaataacaa aggtttttgg agttttactt atgatttgta tagtgcatgc agtagaaaca
    21901 tacatattaa accctaagtt gatgtcgaat agaactaaac ttccagtttg ttttgttttt
    21961 attattttac ttgtaggaga acattattta ggtgtatggg gattactaat tggagttcca
    22021 atatttatgt tccttatgga tattctgggt gttaaattta cttctaggta atatattaaa
    22081 ataataattt atttttatat ataaattatg aattgtttta aaaaagaacc tataaataga
    22141 catcattaat aaagacatct atttgatagg ttcaattttg ctttgtataa atactataga
    22201 attaaataaa aagaaattta tttcatttca aaagtttcac aacttgcatt tctttcattt
    22261 atatgaactt tttcagcttt gcaaagttca tttttattgt atttacattt tacagcttca
    22321 caatgaatgt tatctgtatc aacttggtta gtttcattag aacgactagt tataccagat
    22381 gtatatttgt cttcaaaaga agcacaataa gtattttctg tagtagttgc ttgcattcca
    22441 tctactttta tactaccagc ataacaatat ccactagtat tataagcaca attagtagca
    22501 gcacaattta aattattatt tttcataaat aacctcctaa gttttattta aacttattat
    22561 gtgaagttat ctaaatttta tacacttatt tacaattaca tttatcacat ttaacaacta
    22621 catctttgga agtttccctt cctaaatttt tattaacatg atacacatca acattttcag
    22681 ttgatccaca tatagaacat ttaaaattgt tttctagtag ggttctttct ttaaataact
    22741 tccaatacat agaattgatg taatcattat aatttacctt gaggcatgtc tcaagaaagt
    22801 ttatgtatag attgtctaaa tctcctgtat ttaattctgt atattctggt tcaaaatatt
    22861 cacaacatcc tctaaaatca ggctttgtat ctgaaataac acatctaata tcatatctat
    22921 agtctaggct aggcccctta aatgaaagat gtgtgcaaaa agaacaagat gtttctggta
    22981 gatttttttc aagttcatgt aatttattat tcataagtat tcctccaaaa aatttatttt
    23041 aagtatataa atgaatagat tattttaaaa cttttattta tttatattag agatttagga
    23101 taaatattcc aataaggagt aaatacttaa gtatttatta ctgtattaaa taaaaaagag
    23161 gcaaatctaa cttgagattg cctacttttt tatcattttt taataaaatt tattctaagt
    23221 attattttat ttagatttag tcagccaatt tagtatccgc tttgcttttt aactttctac
    23281 cagagcgtac agttaaaata gcatataata cagtaaatat ggcagctaaa acatatgata
    23341 tatttatgtt taagttaaat ccaatttgag cattaaatat ataactgaat ataacaaaag
    23401 aataaaacat accaggaatt aagcttataa tataattctt ttcttttatt ataaggtaaa
    23461 ctgtaatcat tgcaaatgca aatatggcaa tagtttgatt actccaagag aagtatctcc
    23521 acaatatatt aaatccagaa gcactaagct ttgcaaatat aagaatggct atgacaggaa
    23581 taaatataca gattgacaat ataacacgat gtttcttttc cttttggtca taatgaaagt
    23641 aatctgcaag cattaatctt aaagatctta aagctgtatc tccagaagtt attggtaaaa
    23701 caattacacc tattatagct atgataccac caatagaacc aagtaaatct cttgcaacca
    23761 aacctattac atcaggagaa cctactaatt ccttaggtat tcctttattg taaataccca
    23821 tagcagctgc agcccaaatc attgcaatta aaccttctaa aatcatcata ttataaaaag
    23881 tagtttttcc ttctttttca tttgaaactg atcttgctat aagagtagct tgagttgagt
    23941 ggaatccaga aacgatacca caagcaactg taacaaagaa tgtaggaata agaggaatac
    24001 catcaggatg aatacctttc caatttgcaa gacttaaatt agctaaatca taaccttgtg
    24061 taaagattcc aattccaaca ccagcagcag ataaaagtag aatagctcca aatattggat
    24121 atacctttcc tatgatttta tcaattggaa agagtgttgc acacaagtaa tatagaaaga
    24181 taagtccata aactatccag acaactggat tatttatagt cgatttcata tttaaaattt
    24241 gagtaacaac taaatcccca ggtgtataaa taaaaactgc accaactaaa agcattaaaa
    24301 gacaaaggaa tatattatag acttgaaata ctttattacc taaaaatcta ctgataagag
    24361 aaggcatttg tgctcctttt tctcttatac taatcattcc actcatatag tcatgcaaag
    24421 ctcctccaaa tacacagcca ataggtatta gtatgaatgc tattggacca aacaatatac
    24481 cttggatagg tcctagaatt ggtcctgtac cagctatatt aagcaactca attagactat
    24541 ttttccattt tttcattggg acatagtcta ctccatcact ttgagcaagt gcaggtgttt
    24601 ttctgtcatc tgggccaaat actttttcac aataagcacc gtataaagca cctcctatta
    24661 gtaatatagc caagccgatt aagaatgttg tcataatatc accccattta taaaattttt
    24721 tggtctttgt atacatatta tctaaatgtg tagatttata ttgaatttaa ttctggacaa
    24781 cctacaaatt ctataaagta ttacaactaa tataatttca gaaataaata catatacata
    24841 accatgtact tagtatacag gtatttggta atattttcaa tgttatagca ataaagaata
    24901 taaacaaaat tctgataata ttttacaaaa aaattaaaaa atataaaaat tagtttaaat
    24961 agatggagaa aaaaggaaaa tcaattgaga atataaatgt ctatatctta acaatattaa
    25021 gaggtttaaa attcttgaag aaagaatagt caagtgatat aatgggttta caggtgtata
    25081 tacactagta catatagaaa gcggtgcaat tatggataaa gatttaacat atcaaatcaa
    25141 agagctaaag aaagaaaaaa atgcaataat acttgcccat ttttatcaac caccagaaat
    25201 acaagaatta gcagatgcag ttggtgactc atattattta agtgagattg ctagagattg
    25261 taaagaagaa gtggttgtat tttgtggagt aaggtttatg ggagaaagtg ctaagatatt
    25321 atcacctgaa aaaactgtat tgatgccagt aagtaacgct gggtgtgcta tggcagatat
    25381 ggtagatgaa gaaggcgtga taaaattaaa acaacagtat ccaaatgctt tggttgtatg
    25441 ttatataaac tctacagcta aagttaaggc acattgtgat gtttctgtta catcttctag
    25501 tgcaattaaa atactagaaa acatagataa taaagaaatt atattccttc cagataaaaa
    25561 tttaggagga tatattgcag aacaatttcc agataaaaac tttatatttt gggacggata
    25621 ttgtaaatac cataacaata taagagctga agaaataatt gaattaaaag ataagtataa
    25681 aaatgcagaa gtattagtac atccagaatg taaaaaagaa ataagagatt taggagatta
    25741 tgtaggtagt acatccggaa taataaaata tgctactaat agtaaaaaca aagactttat
    25801 aatagcaact gaagaaggta tattacatga attaaagaaa aataatccta ataaaaactt
    25861 ttatattcct ggtggaaaaa tactatgtac agatatgaaa aagacaacat tagaaaattt
    25921 atatagtaca cttaaaaata tggaaaatga agttattgta gaagatgaaa tcatggaaaa
    25981 ggctttaaat tcactgctta acatgcacaa attagcagaa ggatagaggg atagacttat
    26041 gaatttagaa caagatgttt taatagttgg aagtggagtt tcaggattat actgtgcatt
    26101 aaatttagat aaaagtttaa atgtgctagt agtatcgaaa tcaacaattg aaaacaacaa
    26161 tacatatctt gcacaaggtg gaatttcaac tgctagaaat attgatgata tagaatcctt
    26221 tgtagaagat acaatgaagg ctgggcagta taaaaataga gttgaagcag ttcaagtact
    26281 tgcagatgaa tctatagaaa atgtagggca gatagtagaa tatggtatgc ctttagacaa
    26341 agaaaatgga gagatagatt acacaagaga aggagcacat agtgtaaatc gaatagttca
    26401 tagtaaggat aatactggag aagttgtttt taaaacttta ttaaaagaag ctaaaactag
    26461 agaaaatata actcttatag aagatgcata tcttttagat atattaaaag atggcaataa
    26521 atgtatagga gctagaattt ttaaatctaa aaaagagatt catgtttttt caaaaatagt
    26581 agttttagct acaggtggaa ttggtgggct ttttaaaaat tctactaatc aaagacatct
    26641 tacaggtgat ggaattgcaa tagctttaag aaataatata aaaatagaaa atttagaata
    26701 tattcaaata catcctacag ccttttatga agaaaataat gaaggtagga gaatgcttat
    26761 atctgaatca ttaagaggag agggagctaa acttttaaat aaaaataaag aaagatttgt
    26821 ggatgaactt cttcctagag atgtagtttc aaaagctatt tttgagcaaa tggaaaaaga
    26881 taaattacct tatgtgtatt tagatgcaac acatttagat agtgaatatt taattaatag
    26941 attttctttt atttataatg aatgtttagc tagaggaaca gatataacga aagaatgtat
    27001 aaaggtatct cctgcacaac attattttat gggcgggata catgtagatt tggattcaaa
    27061 aacatctatg gaaaatttat atgcagttgg tgaaattagt tgtacaggtg ttcatggagc
    27121 caatagattg gcaagtaatt cattacttga agggcttgta ttttcaaaaa gagcagctaa
    27181 aaatataaac tcagttatag ataatgtaaa agttaagttt atagatgtac cagatatgga
    27241 tatagatata gaacaggtaa aaaaagaaaa taaaatatta gtcataaaag aaattgaaag
    27301 aacaagtgag gattttggcg atgaattatt tgattattga tagaatgata caggatgctt
    27361 tgatagaaga tgttccaagt gaagatataa caactaattc tattgtggat gaaaattcta
    27421 aatctactgt agatttaatt tgcaaacaag atggtataat agctggctta ggtgtattta
    27481 agagagtttt tgagatactt ggtgatgtag atgtaaaatt atataaaaat gatggtgata
    27541 gagtaaagaa tcgtgaaaag atagctgtat taacaggtag tactagaaac ttacttgtag
    27601 gagagcgtgt agcattgaat tatttacaga gaatgagtgg tatagctact attacaagta
    27661 ggtatgttga aaagcttgaa ggtacaaata caaaattatt agatagtaga aaaacgatac
    27721 ctaatcttag aatattagat aagtattctg ttaaagtagg tggaggatgc aatcatagat
    27781 ttaatttatc tgatggaata ttgcttaaag acaaccatat tggggctgct ggtagtgtta
    27841 agaaggcagt tgaattagct agaaaaaata cttcttttgt tagaaagata gaagtggaag
    27901 tggaaacttt agacatggta aaagaagcaa tagaagctaa tgcagatata ataatgttgg
    27961 ataatatgag tttggaaatg gcaaaacaag cagtagatat tataaatgga agagccattg
    28021 tagaattttc cggaaatgta aatttagata ctatagaaga tataggaaaa attggtgtgg
    28081 atgttgtttc tgtaggagcg cttacacatt ctgttaaagc tctcgatata agtatgaaga
    28141 atttgagaaa tatataagtt ttatattgaa ataaacttaa ttaaaataga ctccttaaat
    28201 ttgttttata aatttaagga gtctattttt tattcattaa aattaataaa aatataaaaa
    28261 aatcaatcaa taatttaata aaaatagata aatactatat acacttttaa taatatagct
    28321 aatatctatt ttacagataa ataagaataa acttataata taattgtatc tgtaaaattt
    28381 tgtaaaatat aaaaagtata gggggaataa aaatgaaagt agcttatatt tttagtagta
    28441 ctaatacaca taaaattcta gacaaaatga taataccaca acttgaacaa ggaactcatg
    28501 gagtagacgt tctagggatg tttttcttta tggataatac cttattttta tctaaaggta
    28561 atccagtagg cgagagacta agtaaattac atgaaaaaac tgatatgata ataatggctt
    28621 gtgaccaatg tgctatagaa agaggtatag agaataattt ggttgatgga gctacaattg
    28681 gatgtttccc aaacctatat gcttgtttag gcagtgcagg tgtggaccaa gttataacgt
    28741 tataaattag tataggatac aatccataga aaaatttatt ttctgtggat tattttaagt
    28801 aaaagataaa ttgtttaaag taggggatga acaaatgtct aaaattttag ttgaatttat
    28861 aaacacttga ccatcttgtg atgaacatgg agtgtttata aaaaaaatgt cagaaaagta
    28921 tgctgataaa ttggaaataa aattatatca agcagggaaa gatttttcgt atataaaaaa
    28981 atatgggatt ataacaaaag gaactttaat aataaatcag aaaaagaaat atgatagatt
    29041 aaataaagat acaattgaaa gggcaatagt agaagctata aacaataatt aaaagtttaa
    29101 aaattaggag tatcaaaaat gaatatatta gaaattgttt taaatggagt taaagatact
    29161 atagtagctt ctatagttac actgaatgga gcatcaggat ggcttatgat aagttttata
    29221 ttggcaggtg tacttcacaa tgttttaact ccaagaaggt ttcaaaaaca acttggaaat
    29281 aagaaatttt cttctttaat taaaagtact atctcaggaa tgtttttacc aatatgtagt
    29341 tgtggagtaa taccacttgg aataagtatg tattattcag gagcatattt aggaccagta
    29401 cttgctttta tgacatcaac tccaataata aatccaatag ctgtagttct ttgtctaggt
    29461 cttttaggtc ctaaagttac aatcatttat ctaatagctg gttttttagt accattttta
    29521 gtaggatttt taggaaattg ttttggaaaa gaagaattat cactttataa tgattcagag
    29581 gaagaagtaa tagaattaga agaagaaaaa agaagtttta tggaaaatat gattgatggt
    29641 cttaaatggt cttttggtga attagccctt actattagta aatatgtagt aatgggtatg
    29701 gtttttgcag gatttataac aactattttt ccaaatagta taattcaaaa atatttagga
    29761 aatccaggtt tgctatctct tggaagtata gcaatacttg catgtgttat gtatgtatgt
    29821 gctgtaggac atataccgtt tatagcagcc cttgttgcaa gtggagcatc tcctggtata
    29881 gcgataacat ttctaatagc tggagcagct acaaatctac cagaacttat aagcatgttt
    29941 aaacttattg gaaaaagaac tgttattata tattctgtaa ctttgacact gtcctcaata
    30001 ataattggat atataacaaa tttactttta gtgaataata attcagtaaa tttagatgca
    30061 acaaattctt caatacagtt ggcaaataaa ctaatgttaa atatacctga tagttttgag
    30121 tatgtatgta ctattttaat tattttattt gcatttaaat caacaatacc aaagatgaaa
    30181 gaggtcatag gaaatcatgc ttaaaaagat agttataata tcctgtatag tagttgtgtt
    30241 aatcatttta tcaaaaatag ttgatgataa catcaaagaa gatgcttcta ttccaaatgt
    30301 gaataaagaa acattagaat attttagaaa aaattataaa gaagacataa taacttgtgc
    30361 tgaagaagat ttaaataatg atgggaaaaa agatttagta gttatatata aaaaaagcaa
    30421 taattcaaat gaaatggtag tcgtagtttc tgataaaaat agccactata taacaaagcc
    30481 aataccagca cctatagaga atcaaactat aacctttaaa aatattgatg ataaagcacc
    30541 aatagaagta atagtatcag ggtctaaaaa tggtaatgta ggatatgcta tttacagagt
    30601 agagggtaaa aaatttgttg atttatttgg cgaagatatg gacaaatgtt gctaaaaata
    30661 aaaataatac ataaaggtgg gaaatttata tgaaattatc taagaagatt caagcactaa
    30721 tcttatttgg tgtaatgggt acatctatat taacaggatg tagtagtaaa ccaaaagaaa
    30781 aagaagaagc aaaagctgga aaagatgatt atacaataac agtaggttat tataattgtg
    30841 accatatgac tgcgggacca gtagcagaat cggcaggaat atataaagat ttaggtctta
    30901 atgtaaaaac tgttggaaat ggtaaagttc ctgaagctat ggcagcagga aaaatggatg
    30961 caggttacat aggaactaag gggttagttg gagcaatacc taagggttca ccaataacaa
    31021 tagcagctaa caaccatact ggtggttctg aatatttaat agtttcaaaa gatataaaag
    31081 aacctaaaga tttaataggt aaaaaaattg caacagatat gagtgatttc ttatggacaa
    31141 gtgattatgg tccagaaaca ggtctaccaa ctgacccatc aaaatatgaa gttgtaaata
    31201 tggattcaga caaagataaa tatttagctc ttaaaactgg gaaaattcaa gcttttacat
    31261 cttgtgaccc ttggggttct gtagctgaaa atgacggtgc aggaaaaata atagcatcta
    31321 ctcaatataa agaaaaagct aatggcaaag aatataactg ttgttcattc tcattaaaca
    31381 aaaactttat aaaagaacat ccagatttag caaaaaaatt ggttctagca catacaaaag
    31441 caatagaata tatatataca aatccagctg aagcagctaa aatatttgca aaatattata
    31501 atgtagaaga agaagttgca cttagaacta tctataagaa gactgttgga gaaggtagaa
    31561 ctcttacttg gaaagttaca ggtgaagaat ataaaaataa tttacaaatg tataaagatt
    31621 taaaggcttt agatgatata cctaaatatg aagatacgat tgatacaagc ttattggact
    31681 cttgtggagc tgatgatttt gacaaattta taaaagaaaa agttaaccca gagtttcctg
    31741 aaggtatgag ttatgaagac tggaaagcaa aggtattata aataaatgga gaatataaaa
    31801 aatataataa tactttttat cattattttg ggaagtatat ttataatatt ttttaatttt
    31861 gaagataaag aagaaataaa aggaaaatta agaatagggg tatctgatga tacctctggt
    31921 tttgtaataa attatatggt aaatagagac tactttgaaa atataaaagt aagtgatata
    31981 atggatattt ttacaattaa tgattgctga gcaagtacaa tgcagtgggc tctgagttca
    32041 gaggaaatag atatagcaat catatgtaaa gatgctgcaa aacaatatgt aaatgtaaat
    32101 agtggatttg aaattgttgg tactgtagtt caaaattctg atatatttct attaagtgat
    32161 aataatcctg aaaagatagg agtcattcaa aatagagatt accagtcaga attggttaaa
    32221 aaatactaca agaatgtgga agtagctcca ttattaggga ctgcactagc atatggatta
    32281 gaaagtaatt tagtagatgg tgttgtaata gattgcataa agtcaattgg gttagagggt
    32341 aaaagaaaat ctacaactaa attaggagat tatgatacct atgtattagt tgtaagtaaa
    32401 gagtttaaaa aaagtaaagc atatagtgat tttataacac tttacaataa aagtgttgat
    32461 gctttaaaga ataaagctac atttaagaaa gcattaaaag agtacaggaa tataagaaat
    32521 atagatatgg aggaaataag caattggaaa ttaaaatttc taaagataaa agcgatagat
    32581 taactaagaa aaaaactgat ggtagtatgg atattttata taaaataata ctattagtta
    32641 ttatattttt agtctggcat tttgcagcaa aagatatagg gagttctttg ttacttccaa
    32701 tgccagtaga tgtaataaaa ggtttctttt tctgtgtgac agatgcagag acagtcacta
    32761 atctttttat aacattgcag agagtactta aaggttttat gtatgcatta ttgtttgggc
    32821 ttccaatagg atttattatg ggattttcta aaacttttga aagggtttta tctccagtag
    32881 tagattctgt aaggcaagtt ccaataatgg catgggttcc tcttactata gtatggtttg
    32941 gtataggaga tggtccaaca atatttttaa tagctttttc aggggttttc cctataattt
    33001 taaatactat tcaaggagta agagcaatat caaaggatta ttataatgca gctagaagta
    33061 tgggagcatc gccaattgtg atatttacaa atgtaattgt tcccgcatct cttccagata
    33121 tactaactgg ttcaaggatt gctataagta caggatggat gtctgttatc tgagccgagt
    33181 ttatagctac gagtgccggt ctcggttact caatggtaca agctcaaaca ttaatgcata
    33241 cagatgtatt aattgggctt atgatatttg cagctttaat aggatttata atagataggg
    33301 tattaaagtt tataaataaa atcctttgta gatggaggtt tgcagattaa tgaagttaag
    33361 tgtaagagat ataaataaga catttgtaaa taatagagta catacaaagg ttttagaaga
    33421 tataagtatg gatatagatg atggacaatt tgtctgtctt ttaggaccat caggttgtgg
    33481 aaaaacaact ttacttacta taataggtgg gtttcaaaaa tcagaacgtg gagatgtttt
    33541 tataaatgat aaaagagtta aaaaaccggg catagataga gcttttatat ttcaaaatta
    33601 tgctttattt ccttggaaaa ctataagagg taatgttcta tttcctatga aacagcagaa
    33661 aataccaaaa gaaaaaagag aagaaatgct agaagaactt ttggttatgt cagacttgaa
    33721 aggaaaagaa aaactgtttc cgcatcaatt atctggagga atgaaacaaa gagtggccat
    33781 gataagagct ttagcatgta atccagaggt attactaatg gatgaaccat taggagctgt
    33841 agattttcaa atgagacaaa atttacagga agagctagaa cgaatatgga taaagaagaa
    33901 aataactgca ttaatggtaa ctcatgatgt tgatgaagct gtatatatga gtgatagagt
    33961 tatagtaatg tcgagagata aaggaagaat tattgatgat ataaatatag atattccaag
    34021 acctagaatt agaggaagcc aaaaatatga agagtacaaa aacaaattaa ctgatacttt
    34081 atcaaagtgt tatgaagttt aaagaggagt agacatgagt caaatagaaa aatacaatag
    34141 tttggataat acacaacaga aaataagaat ctctattatg gatatgataa tagataaagg
    34201 ttctagtgtt acacttcaag aagtgactga atatgtaagt aaaaagttga atatagaaaa
    34261 agaatatata gaaaggacac tacaatattt tatttatgaa aatataatgg ttgttgatgg
    34321 gaatagtata aattttatat atcctgtatc agcattgcaa acaaatcata aagtaacttt
    34381 gagagataat agaagtttca gcgcaatgtg tgctatagat gctataggaa cctcttgcac
    34441 attcaaccaa gatataaaaa taaactctat ttgtagtgct acaggtaagg aaatagaagt
    34501 aattataaaa aatgaaaaga ttgagtatgt gaataatcca aacttaagag tgcttcatat
    34561 aaatcttgat aagcatctaa attgggctgc tagttgctga aatataatga atttcttctg
    34621 gacgaagagt gattttgatg cttggacaaa cgaagcggga ttatctgatg atgaagatat
    34681 atattgttta gatattaatg aggcaatagt agaatcatat aaaatattta aactcaaaca
    34741 aaaggtacta tcatagatat acatatatga aaaactaaaa atgaccttta gataaaaatg
    34801 ataggtaata tccatactat ataaattagt atagagtagg gatgtgaaaa gtatttaaat
    34861 ctaaaggtat atttttagtt tttttagatg attagatagc cttattaagt atatatttat
    34921 ttataacctt tgcaactcca ttttcattat tagtatcagt aacataatct gcaatttcct
    34981 ttatactatt acgtgcattc cccatagcca caccaagacc tgcatacttt atcatatgtt
    35041 ggtcattttc ttcatcgcca acagcaatag cattttctat aggtatattt agattcgtac
    35101 ataatgtttt aagagcagtt cctttattag tagttttacc taaaaattct agataaaaag
    35161 gagcagacct tactatagta tatttattga ataaattttt aggtagttgc tgtatgattt
    35221 cttccaatct ttctggttca tcaactatca taattttaca gattttaata gattcatcaa
    35281 ttttatcttt ttgtatatat ttgacttcta tattatttaa tttcgcttca tgaagtgtat
    35341 attttccagg tgcttcattt ggtgtaatta aatttgtcat tgtatttatg tgtattttag
    35401 tcttatattt tttacaaacg ttattatata taaaatcaaa atcatctaaa gtcatttcta
    35461 catcatgtat gattttctca cttttaactt cttgaataat tgctccatta aagcatataa
    35521 cataatcacc ttcatctgtt aaatttagtt gatttagaag ttcttttaca ccaggaagag
    35581 gtctgcctgt tgttataact acttttgccc cagccctttt tgcctcctga atggactcaa
    35641 agacttcagg agtaatcctt ttttgtgtat ttaatattgt tccatcaatg tctagtgcga
    35701 ttaatttgta catatcaacc tccaataaat tatatactca aatttcctgt gaaaacgttg
    35761 acacagctag tgttgaacaa agtttcagtg ggaaatatgg tttgttttgt atactattat
    35821 atcaagagat aagtaattta gaaagtttaa taatattaat ttttgataaa aatattactt
    35881 atgtgagatg agaatgtaaa aactttgtaa tcaataattc caaaattctt aattacaaat
    35941 aatggtatcg tttgcattgt taatataaat aaatgtatat tatatagtta gaagtttgaa
    36001 taaatagact gatggtaatt tgttatatta gttagttttg tgttataaaa tattgtaatt
    36061 ccattaatca tatgttatta ttcaacatga ttataatata aagaatattt aatatatata
    36121 ataatttata aaaaagtatt acatagtaca tgtaaatcta tatttataaa tacaataaag
    36181 taaagttgta aaggagtgct ttaaatgaag ttttcagagt ttaagtatga aagacccaat
    36241 tataatagta tgaaaaaaga gtttttgagt tgtgtagaag atataaataa ttctcgtaat
    36301 tataaagagc aacaaaaaaa tatacataag attaatttat taagaaataa aatagaaacg
    36361 ttgtcaaata tagcttcgat aagatatagt acagacacat ttaataaatt ctacaaagaa
    36421 gaaaaaaatt attgggatga atatatgcct ttgtatgaag aattaaattc acatttttat
    36481 aatgctattg taaattcaaa atttaaatat gatttgataa aagagtttgg agaacaattt
    36541 ttcacaatag tagagtattc tttaaaaagt ttttctaaag aaataataag tgaattgcag
    36601 gaagaaaata aattgtgttc agagtatact agacttttag cttcagctga gataatgttt
    36661 gatgggaata taagaaattt atctggtatg gggaaattca tgtattctaa gaatagaaaa
    36721 acaagagaat tagcaaacaa agcatattat aatttctttg aagaaaatga aactaaattt
    36781 gatgacatat ttgataaatt ggttaaactt agagataaga tatctaaaaa actaggtttt
    36841 gaagattttg tagagcttgg gtacgttaga atgatgagaa gtgactacag agaagatatg
    36901 ataaaaaatg ttagaaaaca agtattaaaa tatgttgttc caatggccaa tgagctttat
    36961 gaaaaacaag ctaaaagaat tggtctagaa tatttaagtt acatagatga aggcgtggaa
    37021 tttttaacag gaaatgcaag tttaaaggga gattcaagat acataattca aaatggtaaa
    37081 aggatgtatt ctgaactgtc aaaagaaaca aatgaatttt ttgattttat gttagaaaat
    37141 gaacttatgg atttagaaac gaaaaaagga aaaggagctg gagggtattg tacttatata
    37201 ccagattata agtcaccatt cattttttct aattttaatc aaactgctga tgatatagat
    37261 gtgttaacac atgaggcagg acatgcattt cagttgtata tgtctagatg gattgatatg
    37321 ccagaaatta attttccaac gcttgatagt tgtgaaatcc attcaatgag tatggaattt
    37381 ataacatggc cgtggatgga tttatttttt aaagaagata cagataagta caaattcact
    37441 cacttatcat cttcaattaa atttatacct tatggagtaa ttgttgatga atttcaacac
    37501 tatatttaca aaaatcctaa tgtagataag tcaaaacgaa aagaaatctg gagattttta
    37561 gagaaaaaat acttacctca tagaaaatat ggtgataatt cttttttaga gagaggttgt
    37621 tggtggttta aacaaggaca tatatttaaa aatccgtttt attatataga ttatgtatta
    37681 gctcaaattt gtgcgttaca attttggaaa aaaatgattc aagatagaga tgcaggatgg
    37741 aaagattata taaatatctg taaagttgga ggaacaaaat catttttaga tattgtgagt
    37801 atgggaaatt tgtattctcc atttgatgat ggctgtattg agtctattat aggtgatgtt
    37861 aaatcttggt ttgatgaaat aaatgacagc aaattataaa aaattatgag aattttattg
    37921 tataaaagtg aaaaattaaa aaatatataa aaagtgatta tttgttgtga aaatagtctt
    37981 gtttttaact aaattgataa aataaactaa aaaagatttg tacttaaaaa aaaaagaaaa
    38041 tacaagaaat atgcaatatc aacatttgga aaaaaaagta aaaatgtata caaaatacat
    38101 ataaaaaaac tattgcaaat tttatagaat gtttatatac ttaagtcaac agagatagaa
    38161 aaaagctatt tatagaaaac aaaggataaa ttgcttaatt ttaaattttc tgaaaaaaat
    38221 aataatccgg atgaagagta tgggagactg ctttataaaa agcgaccgaa gggacaagtt
    38281 taatcttact tacccaaaag attagatgaa attctcaggt aaaggtgact gtacttggac
    38341 ggggctctga aaatgatttt aacagggaag acaaattggg agatttgttt ttcctgtttt
    38401 ttatttatca agtaataaat aaaagtttga aagagagtaa aaaaatgata ataattacga
    38461 ataatccaaa agtaaaagaa gaagttcaag gcagagaagt tttgtttaaa gacacaactt
    38521 atattggaat cttagaagca agtagagatt tgatacatga aggttatgaa cttttatctc
    38581 atccactcta tgggagtgta aaaccaaatg aaacacctta caggactgtt atactaaaga
    38641 aaggaaatcg tttggatata aactctttaa ccttaattga agaagcaatc atcactgcaa
    38701 gtaaatttca ggataataaa aagactccaa aatggacaga aaatgtccaa gacgatttta
    38761 gagtaattga ttatgatata ttttataata caatacaaag aatgcagtat gaataaaaaa
    38821 atttgttaaa aagttattga aacagcctaa atattaaatc taatttaata ttaaataaaa
    38881 tgagaaattt aattggggag gataagtcaa aatggaaaat gtgtatgact tagtaataat
    38941 tggttcagga ccagctgggc ttgcagcagg tctttatgga gcaagagcaa agttaaaaac
    39001 tctaatccta gaaaaagaca agactggtgg acaaattgta ataacacatg aaattgcaaa
    39061 ttatccaggg tcagttccaa atgcaacagg accaagttta atagctagaa tggttgaaca
    39121 gtgtaaagag tttggagcag aaatgctaag agataacatt gttgatactg agctagatgg
    39181 agatataaaa gttttaaaag gggaaaaggc agaatataga gcaaaagcag taataatagg
    39241 aacaggagca acaccaagaa agattggttg tcctggagaa aaagaactta ctggtaaagg
    39301 ggtttcatat tgtgcaactt gtgatgcaga tttctttgaa gattttgaag tatttgttgt
    39361 aggtggagga gacagtgcat tagaagaagc aatgtattta actaaatttg ctagaaaagt
    39421 aacaattgta catagaagac aaggatttag atgtgctaag agtgttgaag aaaaagcaaa
    39481 agctaatcca aagatagagt ttttattaga tacagtaatt gaagaaatca aaggtgatgg
    39541 aatattagaa tcagttgtat ttaaaaataa agttactgga gaaactcatg aatactttgc
    39601 agatgaagaa gatggaacaa tgggagtatt tgtgttcgtt ggattagatg ctcaaactga
    39661 tttattcaaa ggtaaagttg atatggatga aaaaggttac ataataactg atgaagatat
    39721 gagaactaac ataccaggcg tatttgcagc aggagattgt agatctaaga ctctaagaca
    39781 ggttgtaact gcaactaatg atggcgcaat tgcatctata gtagctgaga aatacattga
    39841 tgaaaaattt gggaactaga atctgtaaat taagaaatag tgtctgtaaa tttaatgaat
    39901 aatatttaaa attaaaataa aaatttcaag ggggaataag aaaatgttag atttagataa
    39961 ggctacattt gaagaagaag ttttaaatgc agaaggtttt gtatttgttg atttttggag
    40021 tgaaggttgt gagccttgta aagctttaat gccagatgtt cataaattag ctgagactta
    40081 tggggataaa ataaaattct gtaagatgga tacaacaaaa gctagaagat tagctataaa
    40141 acaaaaagta ttaggtcttc caactatggc tatatacaaa gatggagaaa aagtagacga
    40201 agttactaag gatgatgcta cagttcctaa tatagaaaac atgattaaaa aatatcttta
    40261 atatgttgat aaagtattaa cattaaaaga aaaaatctat ttttatctga agataagaag
    40321 aaaaaggttt ttatgtccaa ctttgttaaa aaaataattt taaataaaat aattttttta
    40381 caataaatat tttttagtaa taaaataaag atttaaggca taaaaacaca ataatatatc
    40441 tttaaaaaaa taagataaat tgtaaaatga tataattttg agaaaaactc aaatgcatat
    40501 acacaaatta aataacagga ggtgcaagta tgcgtcttga attagggaaa atctttataa
    40561 aagatattca atttggtgat gtaacagaag taaaagatgg agtattattc atcaataagc
    40621 aagagatgct acaagaaatt ggtggagatg agcatattaa atctatagat atagaattag
    40681 ctcgtccagg tgaaagcgta agaataacac ctgtaaaaga tgttattgag ccaagagtaa
    40741 aagttgaagg taatggggga atcttccctg gtatcatgtc taaagttgat actgtaggag
    40801 aaggaaaaac tcatgcttta aaaggtgtag ctgttgtaac tactggaaaa atagtaggtt
    40861 tccaagaagg tataatagac atgactggag aaggagctaa gtatactcca ttttctaaat
    40921 taaataatgt agtagtaata gctgaaccta ttgatggttt aaaacaatac gctcatgaaa
    40981 aagctgttag aatgatagga ttcaaagcag caatgtactt aggagaagta gcaagaaatc
    41041 ttactccaga tgaagtttca gtatatgaaa ctaaacctct tcttgaatca ataaaagaat
    41101 atccagagct tccaaaagta ggatatgttt acatgcttca gactcaaggt ttattacatg
    41161 atacttatgt atatggagta gatgcaaaac aaatagtacc aactttatta tatccaacag
    41221 aattaatgga cggagcaatt gtaagtggta actgtgtttc tgcatgtgac aaaaacccaa
    41281 gttatgttca tataaacaat ggggttgttg aagacttata tgcacgacat ggtaaagata
    41341 taaactttgt aggtgtaatt ataactaatg aaaatgttta cctagcagat aaagaaagat
    41401 cttcaaactg gacttctaaa ttatgtaaat atttaggatt agatgcagtt atagtttcac
    41461 aagaaggttt tggaaatcct gatactgacc ttataatgaa ctgtaagaag atagaaatgc
    41521 aaggtgttaa aacagttata gttacagatg aatatgctgg acgtgatgga ggttctcaat
    41581 cacttgcaga tgcagatgta agagctgatg ctgttgtaac aggtggaaat gccaaccaag
    41641 ttgtagttct tcctaagtta gataaagtta ttggacattt agaagtagta gatgtaatag
    41701 ctggtggttc tgatggaagt ttaagagctg atggaactat tgaagttgaa atccaagcta
    41761 taacaggtgc aactaatgaa acaggatttg gtcatttaac tgctaaagga tattaagaag
    41821 tattttatta aacgattgag taatgttaat aaaacatatt atagataatt ataataagtg
    41881 ttatagatat ataaaaatat attagaaata taataaggag gattattatg agtttactta
    41941 gtaataaaaa ggttcttata ataggtgacc gtgatggtat accaggacct gcgatagaag
    42001 aatgtgtaaa aacagtagaa ggagcagagg ttgttttctc atctacagaa tgctttgtct
    42061 gaacagctgc tggggctatg gacttagaaa atcaaaacag agttaaagat gctgctgata
    42121 aattcggagc tgaaaatgtt gtgattttac taggtgctgc tgaagccgaa gctgcaggtc
    42181 ttgcagccga aacagtaact gctggagatc caactttcgc tggaccactt gctggagttg
    42241 ccttaggatt aagtgtttac cacgttgttg aggaaccaat aaaatcatta tttgatgaaa
    42301 gtgtatatga agaccaaata agtatgatgg aaatggtttt agaagttgaa gaaatagaag
    42361 aagaaatgtc tggtataaga gaagaatttt gtaaatttta atgcctaaaa gcaaaagggg
    42421 tgaaactaag agatgggaaa actaaaagca gtacattata ttaatcagtt ctttgctggt
    42481 ataggtggag aagaaaaagc tgatactaag ccacatgtgg cagaaacttt acctccaata
    42541 agtttacaac ttgataaatt attaggtgaa gatattgaaa tagttggaac agtagtttgt
    42601 ggagatagct atttcaatga aaatattgat agtgcaagtg aagaagtttt atcaatggta
    42661 aaaggttttg aaccacaact tttcatagct ggtcctgcat ttaatgctgg tagatatggg
    42721 gttgctgctg gaacaataac taaagttgta aaagatgctt tgaatatacc agctttaaca
    42781 ggtatgtatg ttgaaaatcc aggagcagat atgttcaaaa aggatgttta tgtagtagaa
    42841 acttctgatt ctgcagcagg tatgagaaag gcacttccta agatagctaa acttgcagta
    42901 aaacttgcta atggagaaga aattggaact cctaaagatg aaggatacat agctagagga
    42961 ataagagtta actacttcca tgaagacaga ggttctaaga gagctgttga catgttagtt
    43021 aagaaaataa aaggtgaacc atttgaaact gaatacccaa tgccaaactt tgatagagtt
    43081 gacccaagta aagctgtaaa agatttatca aaatgtaaaa tagctttagt tacttctggt
    43141 ggtatagttc caaaaggaaa tccagataga atagagtctt cttcagcttc taaatatgga
    43201 acttattcta tagcaggagt tatggattta acagaagaga cttatgaaac tgcacatggt
    43261 ggatatgacc cagtgtatgc aaatttagat gcagatagag tattacctgt tgatgttctt
    43321 agagatttag aaaaagaagg tgtaattgga aaattacatg agactttcta tacaactgta
    43381 ggtaatggta catctgttgc aaactctaaa aaatatgctt ctgaaattgg agcagcatta
    43441 gtggctgatg gtgtagatgc agtaatatta acttctacat gaggtacttg tactcgttgc
    43501 ggtgcaacga tggtaaaaga aatagaaaaa actggacttc cagtagttca tatgtgtaca
    43561 gtagtaccta tttcattaac agtaggggct aatagaatag ttccaactat agcaatacct
    43621 catccacttg gaaatccagc gcttgaccct acagaagaaa aagccctaag aagaggcctt
    43681 gtagaaaaag cattaaatgc tttaactaca gaagtagatg gtcaaacagt atttgaaaaa
    43741 taaaaataac acacacatat attatataga taaaaatata aacacacact aaaaataaaa
    43801 aataactata cacaaaaaaa ataatataga tttcatgaat ggataaattg gagttattca
    43861 ttcatgaaat cttaaaaaat gccaaaatac ataaacaata atagaatcaa atatacaata
    43921 aaatacaatg attagagctt ttaataagtt taataagatt aaaaaataag tgttcataaa
    43981 atttatggag gtgcacaaaa tgacttatcc agttttaaaa ggggcaggct atgtacttat
    44041 tcatactcca gatatgatag tgcaaaatgg aagtacttgt acagttgaaa gagcaacaaa
    44101 ttcagattca gaatttttaa aagaagtaag taatcatata agaagttatg aagatgtagt
    44161 taactacatg ccaaatcaag tttatatagg aaatagaaga ccagaagaat taagagattt
    44221 accaatgcca tggtgtgaac aaaagattga gggaactaga aatggtaagt ttggagaaat
    44281 aatgcctcaa gatgagttca tagctttaat gcaaataagt gatgcatttg atttagtaaa
    44341 attatcacaa gaatttattg atgaagtaaa gccaaaaatc gaaaataact accctgaaat
    44401 tgcaccattt gtaggtaaat taaagggtga tgatatagaa gaaggtaaag aattagtagc
    44461 aacacacata gctgaaggat tataccatga tggaaaattt gttggttatg taaaaagagc
    44521 acatgatgta gatgttaact taaatgccca tacaatgttt gaaaatctag tagtaaaagc
    44581 atctggagtt ttatctgcta ttcaaatgct aagacatagc aaaatagacc ctgctgaaat
    44641 agattatgtt attgagtgtt cagaagaagc ttgtggagat ataaaccaaa gaggtggagg
    44701 aaactttgct aaatcaatag ctgagatagc aggacttcag aatgcaactg gttctgacac
    44761 aagaggattt tgtgcagcac caactcatgc attaattcaa gcagcagctt tagtaaaagc
    44821 tggcatacat aaaaatgtaa tggtagtagc aggtggagca agtgctaaac ttggaatgaa
    44881 tgctaaagac catgttaaaa aaggtcttcc tgtacttgaa gatgttgttg gaggatttgc
    44941 agtacttgta tctgaaaatg atggtgtaaa tccagttata agaactgatt taacaggtaa
    45001 gcatactgtt ggaactggtt cttcaccaca agcagttatg acagcactta taacttcagg
    45061 tcttgataga gctaatttaa aaataacaga tgttgatgtg tattcagttg aaatgcaaaa
    45121 cccagatata actaagccag caggagcagg agatgttcca gaagcaaact acaaaatgat
    45181 aggggcactt gctgttaaac gtggagactt agaaaagaaa gaattgaaag attttgtttc
    45241 taacaaagga ttaccaggat gggcaccaac tcaaggacat ataccatcag gagctcctta
    45301 tataggattt ttaatagatg atttaactac aggaaataga aatagagcta tgatagttgg
    45361 aaaaggtagt ttattcttag gaagaatgac taacttattt gatggagtat cttttatagc
    45421 tgaaagaaat acaggtgtta ctgaagagac ttcaggaata tctaaggatg aaattaagaa
    45481 gataatagct gaatctatga aaaaactagc attagatatg ttagaagaat aggggtgagg
    45541 atatgtcaaa aaaagtaatc gcagatgtat tcttagaagt agctaatgca atagaaagcg
    45601 gcgaatttgg caaaaaagta aaaattggtg ttactacact tggaagtgaa catggtgtag
    45661 aaaacatggt taatggtgca caattagcaa aatctaattt atttgatatt gtgcttattg
    45721 gaccaaaagt ggaaactgac cttgaagtag ttgaagttaa tgatgaaaaa gaaatgcatg
    45781 caaaaatgga agaattatta gattcaggat atattgatgc ttgtgttact atgcattata
    45841 attttccaat aggtgtatct acagtaggta gagtaataac tccagcaaaa ggaaaagaaa
    45901 tgatacttgc aacaacaaca ggaacaagtg ctactaatag aatagaggca atggtaagaa
    45961 atgccattta tggtattgct acagctaaat caatgggtaa caaatgccca aaagttggaa
    46021 tacttaatgt tgatggagca agacaagttg agaagtgctt aaaagaatta aaagataatg
    46081 gatatgacat ggagtttgcg gattcaatta gagcagatgg tggatgtgta atgagaggta
    46141 atgatttact tgtaggtgca cctgatgtta tggttactga tacattatct ggaaatatat
    46201 ttatgaaagt attttcttca tatactacag gtggagatta tgaagctcaa ggctttggat
    46261 acggacctgg agttggagaa gactatgata gaaaggtact tatagtatca agagcatcag
    46321 gttctccggt agtagcaaat gctttaaaat acgcttatga tgttgtaaag ggagatataa
    46381 gtaatgttgc aagaaatgaa tttgcaaagg ttaaaaaagc aaaatttgat gacattatat
    46441 cctctcttac taaaaaagaa gttaaagctg aaaaagtaga agttaaaatg ccagataaag
    46501 aaattgttac tcgtcaaata gctggtgttg atataatgga tttagaagat gcagtatctg
    46561 agttatggaa aaacggaatc tatgctgaaa gtggtatggg ttgtacaggt ccaatagttt
    46621 tagtaaatga agctaaagga gacttagcag ttgaaacttt agttaaagct ggatatactg
    46681 caaaataagt acatttttta tatgtaaata agaacatata gttataattt tattgattat
    46741 aattatatgt tctttttatt ttatatcttg tcaatattta tatattaaca caatccattt
    46801 atttgattct ctagctaatt tattatattt attcaattaa aattaagctt tctaactaaa
    46861 taaaaactaa tgaagtatta aaatgaaatt aataaaatat gaaaaaattt taggaataaa
    46921 gtatctgtta gataaaaata agattaatta gtgatataat ataattaaga taaaatttat
    46981 tatttggagg taaaatcatg agcagagtaa aaaatgtagt aggattgttg ggaactaaag
    47041 gtgttgatgc actatatcta acaaagaaaa ctaatgtaaa ctatataagt ggttttcctg
    47101 atgaagaagc atatgcagtg atttgcaaag atggaaactt tttagttact gatagtagat
    47161 atatggagct tgcagaaaag gtttgcaaag attttgaaat tataaattgg cataattttg
    47221 atagaagtgt ggcaaaggca gttaaaagtg tatgtgataa agtgggtata aagaaacttg
    47281 ggtttgaaag aactaatata gtgtttgata aatatgaaga acttaaaaat ctgattgaga
    47341 aggataatgg agaattaata cctacagaaa atattgtaga gactttgaga tatgtaaaag
    47401 acaaagatga aatcaaaaat acaagaaaag cttgtgaaat agcggataag gcattagaag
    47461 aattgattcc acatataaaa gctggagtta gcgaaattga actggctaca aagttagagt
    47521 actttatgaa aatgaacgga gcacaaaata tagggtttga aactatactg atatcaggag
    47581 caaagacttc attgcttcat ggtaagccta gtgataagat tatagaaaaa ggtgattttg
    47641 tacttataga ctatggtgct atgtataatg gctatatatc tgatacaact agaactttta
    47701 tagttggagg agcttctgaa aagcaattag aaatatataa tttagttaag gaagctcaaa
    47761 atgtaggggt tgagaacatg aaagctggtg tacatgcgac aattccagat gctgagataa
    47821 gaaaagttgt caaaaaatat gaagactatt actatcaagg tataggacat ggagtcggta
    47881 gggatgttca tgaagaacct ttcattggta attatggtga taaaatcata gaagagggat
    47941 gtataataac aatggaacca ggtatttatt tccctggatg gggtggagtt agaatagaag
    48001 atactgtttt gattactaaa aatggtccag aaagacttac taaattccct aaagatttga
    48061 tgattttaga caaataattt aatttatata tattttttta cataaaatat aacataatct
    48121 cactattaag ctctatttac atattatagt aaagagggct taattttatt gtaagaggtg
    48181 atagtatata tgtataaaaa acataaattt ataaaaaata taaaaaaagc ctggtataaa
    48241 atcaaattaa ttgatagatg ccttataatt gtaatggcaa tattaatgtt tcaatctata
    48301 tataatttat ttgttaatga agtaaattca caagatacta caactattga tgtagtagta
    48361 agaaccactt cagcagctat atttgggtat tttttaagtg ctaattttat aaagagacct
    48421 ttaaggaagt atacaaatac tgatgtatct tcaaatcttt ttatagataa taatttaccc
    48481 ccaaaaggtc aagagtctag tcaaaataat ataatgaatg tcaaaaatac cattggattt
    48541 acttcagaag ctgataatta tgaaaagaaa atacctatta ataataatga aggttttgaa
    48601 gaaggagaaa ctagtgaatt gcaaattatt atagctactg taatttgtat agcagcatta
    48661 acagtacttt ttgttgtaag aaattttaca actacaacat cagcttcttt aggtactatt
    48721 tcacagatga gagattttgt ctcaggatgc gtgggttttt tgctaggttg tccaagtaaa
    48781 cctgattaaa aaagtattat caataaaagg tattttaaaa agtatcagta taataatatt
    48841 tagatttaaa tgtatacaat tataattcta taacattcta tattgtattt ttatactgat
    48901 actctaaact atactattta ttaaaatatt ttaacattgt atttaaacaa aatattatat
    48961 ctgtataata taaatttagt ttatatgaat agatgccata tactcaataa aaagtttcat
    49021 actatgagat atgtattgat ttcttctcca aagaactcca atatttgcag taattgcacg
    49081 ttctaatggt attgaacaga gattttctgt attttgaaat atttctttat acaatattgt
    49141 tgaagccaag tcattttcta gtaaataacg tatggtagaa agttgatttg agtgtaaaat
    49201 tatatttggc ttagcttttt caagtctttt tgtaataatc ttatagtgat aagaaccttc
    49261 agataagatt acaaaaggta cattttttaa catataattt gatataatgg gatgttttgc
    49321 caattcattt ttgggatttg taactaagta caattctgtt ttatagattg tctttgaatc
    49381 tatatcatca taaatatcac tgtcaattat accaatacaa aaatcaagtg tagattcttc
    49441 aatcatttta gctccaacaa atgttggaac ttcaaaaatt ttaagctcta tatctggata
    49501 aattacatta aatctaggaa gtatttttga aagcaaaaac gtacctaaaa ctgtaggaat
    49561 acctattttg agagaagctt ttctcttttt tcctaaatca agtgcgtcac attgaaaatc
    49621 ctcaaattgt tttataaaat gttttgactt gtctaaaaaa atttttcctt caggcgttaa
    49681 ttctatttta ttacctattc tgtaaaaaag ttcaaagcca agttcttttt ctaattcttt
    49741 aattgctaat gttatggatg gttgagaaat atgaatactt tccgcagcct tagtcatgtt
    49801 tttatattta cagacagttt gaaaatagta taaatagttt atattcattt ggtattagtt
    49861 cctttcattt acatattagt gacaagtata taattaacaa tagcataagt tttgtatatt
    49921 gttgtaaaaa aatagaatag ttttaaacta tcatagcata agtttttgat attttcaatc
    49981 tattaagaat agtgctatta ttaaattaac aaaagagaat agctggtatg gtttagagta
    50041 aatcaatcta tattacagtt atatcaacga atatcaaaaa attatctatt agaaaataaa
    50101 aattattttt acaattaaat tgttaattaa atcataaaga aaaaagtgat attaacaaga
    50161 taaaattttt ttaatcagaa atgttaattt aataacaaaa tagagtattg gggagaaagg
    50221 agaaatatat tttttaataa tatttgttaa tttaataacg aaaaatgaaa gggagtgatt
    50281 tagtgaagtt tgaattgatg gattttttaa tgaatccttt tgtccttatg tttgctgcag
    50341 taattacagg aattttattt ggaaaaatta aatttgggaa gtttaatttt ggggtatcag
    50401 gagcattatt tacaggatta tttattggat ggcttgcata tagcttgggg aatctaataa
    50461 tagcaaaagg agaaacagca gcaggatata aggcagcaac tgtaatgatg gggaatggaa
    50521 ttatttcttc tgacttcttt gatttctttt taataatatt tgttgcagca gttggtctat
    50581 tggcagcaaa agatatgaaa gctgtattaa agaagtatgg agcaagattt gtaattttag
    50641 gagttcttat aacttttata ggtggctcta tgacttatgc aatgacttta ctatcaagtg
    50701 ataaaggaag tagtgcttat gaagtttctg gcgtttacac aggagcatta acaagttcgc
    50761 ctggattagc tgctgcttta gaaacagctg gtaaacatgc agaagatgta tcaaaagaat
    50821 ttgaaaaagc ttcaataaaa gataaaaaag aaattttaaa agtagtcgac ccagaaggca
    50881 aattggatgt gaatacaact acatcactga ctcaagagca aatagataag tatattgcat
    50941 atgctgaagc tggtgttggt ataggtcatg cagtcgcata tccttttgga gttttaatag
    51001 taatcttagg tgtaaatttc cttccaaaat tatttaggat ggacctaaaa gaagaaagaa
    51061 gaaaatatga gaaggaaatg aaggaagcaa gagatagtgt ttctggtaaa acgataccag
    51121 aagttccatt taatataata acatttttct tgacatgttt agctggatac ttagttggag
    51181 gaatacatgt atttatggga ccactagggt acttcacact aggcgcaaca ggaggttcac
    51241 taattgtatc tctagttctt ggatatatag gtaagatagg ggtagtaaat ttcagaatgg
    51301 aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt atttttctta gcaatagttg
    51361 gattaagata tggtggaaaa gtagttgatt caattatgac atctggtatg cacctagcct
    51421 tagtagcaat agcagtaggt gttacagcta tgatgatagg attcttagtt ggcaaatacg
    51481 tatttaaatt aaattggatt ttattatctg gagctgtctg tggtggtatg acatcaacac
    51541 caggtcttgg agctgctgtt gatgctcttg atagtgatga cccagcagca ggttatggtg
    51601 ctacgtatcc atttgcatta ttaactaaag ttatattggt tatagtttta cacaaacttc
    51661 caatgtaaat aagattttat atggggggat atttatgaat aatgaaggag catttgaaga
    51721 attaagtgga agattaagaa ataaagaatt gtgtgattta ataatgaatg cagatgaagc
    51781 tgctaaaatt atagaggatg gcatggttat tggagttagt ggatttacac cttcaggcta
    51841 tccaaaagca gtaccattag cagtttcaga aagagctaaa agtggagaaa agataaaatt
    51901 aaccgtatat tcaggagcat ctttaggccc agaagttgat ggagcatggt cagaagcagg
    51961 tataattgaa agaagacttc cttatcaaac taattcaata cttagaaata atattaataa
    52021 aggtgttgtt gattatatag atatgcacct aagtcattct actcaatttt taaattatgg
    52081 aactatacct aaagtagatg tagctatagt agaagcactt gcaataactg aagaagggaa
    52141 tattatacca actagtggga taggtaattc tccatctttt ataaaaagtg cagataaagt
    52201 aatagtagag ataaatcttg caaaaccaat ggaaatggaa ggtatggcag atatatatat
    52261 tacggaaaat ccaccaaata gaaaaccaat agaaataaat catcctaagg atagaattgg
    52321 aacaacatat ataccttgtg gtttggataa aattgcagga gtagtaatta ccaatatgca
    52381 agataagacc cgtccattgg gggttgtaga tgaagcttcg aaaaaaatat caaataatat
    52441 tattgctttt ttaagagaag aagtaaaggc aggcagatta tcaaagaatt tacttcctct
    52501 tcaatctggt gtaggaagtg tagcaaatgc agtattatat ggattatgtg aatctgaatt
    52561 tgaaaattta acttgttata cagaggttgt acaggactct atgttggatt taataagaat
    52621 gggtaaagtt acaatggcat ctactacatc tgtaagtcca tctccagaag gattaataaa
    52681 atttgaaaaa gatattgatt tttttaagga taagattata ttaagacctc aagaaattag
    52741 taacaatcca gaaatagcca gaagaattgg tgtaattgca atgaacactg caattgaagt
    52801 tgatatttat ggaaatgtaa attctactca cataatgggg tcaaagatga tgaatggaat
    52861 tggaggttct ggtgattttg ctagaaatgg agcaataact atatttagta ctgagtcgat
    52921 tgcaaaaaat ggtgatatat catcaatagt acctatggta tctcacgtag accacactga
    52981 acatgatgta atggtaattg taacagaaca aggatatgct gatttaagag gtcttgcacc
    53041 tagagaaaga gctataaaaa taatagaaaa ttgtgcccat ccagattata aagaacaact
    53101 tagggattat ttaaatagag cttgtaaaag tggagcaaaa caaacacctc acatattgga
    53161 cgaagcttta tcatggcata gtaaatttat gagtacaggt acaatgaaaa aggctgaaac
    53221 ttttaaaagt gcattataaa atatttaata aattggaggt agattttaaa tggaaaaagc
    53281 agtggaaaac tttgaagatt taagtaagga atacattaat ggttatattg agagagcaag
    53341 aaaagctcaa agagagtttg aatgttatac tcaagaacaa gtagataaaa tagtaaaaat
    53401 agttggaaaa gtagtgtatt ataatgctga atatttagct aaacttgctg tagaagagac
    53461 tggaatggga gtatatgaag ataaggtagc taaaaataaa agcaaagcaa aagttatata
    53521 taacaattta aaagataaaa aatcagttgg tataattgat atagatagag aaactggaat
    53581 aacaaaggta gctaaaccag ttggagtagt agcggcaata actccttgta caaatccaat
    53641 cgttacacct atgagcaatg caatgtttgc acttaaggga agaaatgcaa ttattatcac
    53701 acctcatcat aaagcaattg gatgtagtac aaaaactgta gagatgatta atgaagaatt
    53761 agaaaaaata ggagctccag aaaatttaat acaaatactt gaccaacaat caagagaaaa
    53821 tacaagaaat ttaatttcat cagcagatgt agtaattgcc actggaggaa tgggaatggt
    53881 aaaagctgcg tactcaagtg gaaaaccagc acttggtgta ggggcaggaa atgttcaatg
    53941 tatcatagat agagatgtag acattaaaga agcagtacct aaaatcatag caggaagaat
    54001 ttttgataat ggtatcattt gttcaggtga acagtcagta atagtagcag aagaaatgtt
    54061 tgataaaata atggatgaat ttaaaaataa caaaggtttt atagttagag ataaagtaca
    54121 aaaagaagct ttcagaaatg ctatgtttgt aaataaatct atgaataaag atgcagtagg
    54181 tcaatcagtt catacaattg caaaaattgc aggtgttgag ataccagaag atacaaaaat
    54241 aatagtaata gaagctgatg gaccaggaga agaggatatt attgcaaaag aaaaaatgtg
    54301 tccagttata tctgcataca agtataaaag ttttgaagaa ggtgtagcta ttgcaaaagc
    54361 aaatttaaat gtagaaggaa aaggacatag tgtatctata cattcaaata cagttaaaaa
    54421 tatagaatat gcaggagaaa atatagaagt atcaagattt gttattaatc aatgttgtgc
    54481 tactagtgct ggaggaagtt tctttaatgg tcttgcacct actaatactc taggttgtgg
    54541 ttcttgggga aataacagca tctctgaaaa tcttgattat aagcatctaa ttaatatttc
    54601 tagaattgct tattatatgc cagaaaatga agtccctaca gatgaagaac tttggggata
    54661 aaacttagac aaaaaatatt cattctaaat taaagtagga atatatttta atttagaatg
    54721 aatttaagac ataaattaaa tttaaattag tttattggac taaatttaaa atattaagag
    54781 acatatagaa gaaggaagta ttataattta aaataaataa aaaaatctat aaaagagaag
    54841 gagtggtttt attatggcat taatgacagg agctcaatat attgaaagtt tgagaaaatt
    54901 aaatactaag gtttatatgt ttggtgaaga ggttaagaat tgggttgacc atcctatgat
    54961 tagaccatct ataaattgtg tagcagcaac ttatgactta gctcatgacc cagaatatgc
    55021 agatttaatg acagttactt caaatatcac aggtgaaaaa ataaatagat ttggtcatct
    55081 tcatcaaagt gttgatgatt tgataaaaaa agtaaaaatg caaagactat gtggtcaaaa
    55141 aacagcttct tgtttccaaa gatgtgttgg tatggatgcg tttaatgcag tgtattcaac
    55201 aacatttgag tgtgataaag cccatggaac taattatcat gataattttg taaaatactt
    55261 aacttatata caagaaaacg atttagtagt tgatggagct atgactgacc ctaagggaga
    55321 tagaagtcta tcacctagtg ctcaacctga cccagatatg ttccttcata tagtagaaag
    55381 aagagaagat gggataattg taagaggagc aaaggcacat caaactggtt caataaattc
    55441 acatgagcac cttataatgc ctactataag tatgactgaa gcagataaag actacgctgt
    55501 atcatttgca gttccttcag atgctgaagg tgtgtttatg atttatggaa gacaatcttg
    55561 tgacaccaga aaattagaag aaggtgcaga tgttgattta ggaaataagg agtttggagg
    55621 tcaagaagct ttagtagtat ttgataatgt atttgttcca aatgatagaa ttttcttatg
    55681 tggagaatgg gatttctctg gtatgttagt agagagattt gcaggatacc atagacaaag
    55741 ttatggtgga tgtaaagtag gagtaggtga tgtaataata ggagcagcag ctttagcagc
    55801 tgattataat ggagcaaaca aagcttcaca tataaaagat aaactaatag aaatgactca
    55861 ccttaatgaa agcttatatt gctgtggtat agcatgttca tcagaaggtc acaaaacaga
    55921 agctggaaac tatcaaatag atttacttct tgcaaatgtg tgtaaacaaa atgtaactag
    55981 atttccttat gaaatagtaa gacttgcaga agatatagct ggagggctta tggttactat
    56041 gccttcagaa aaagacttta aatcagacct taaagttgga acaagtggaa tgactattgg
    56101 cgaagtatgt aacaagtatt ttaaagcttc ttcagtggct tcaacagaag aaagaatgag
    56161 aatattaaga ttcttagaaa atatatgtct tggttcatct gctgttggtt atagaactga
    56221 gtcaatgcat ggggcaggtt caccacaagc tcaaagaata atgatttcaa gacaaggaaa
    56281 cataaatcaa aagaaagaat tagctaagaa aattgcggga ataaaaaaag aggaagcttt
    56341 aaatttataa aaagttgata tataaaggga aattatatat taatgtgtat taattaatga
    56401 aaagatatgt agtataagag atagttataa aattatggtg gtttttagct aagtaaagaa
    56461 atttttaata aggagaattt acacatgacc tgtggagtta gattttgtgg gggctgtaac
    56521 cctagatacg aaagagggaa ggctcttaca ataatcaaag agcacttcaa tggtagggta
    56581 gattttagca tagcacaaga gaacgtcgag tatgatttgc tacttataat tggaggatgc
    56641 gcaactctat gcgcatccta tcaacaatat aaagtgaaaa atgaatgtgt taagatgtgg
    56701 gattttagcc atatagaaag agttataaat gtgattgaat cagcaatttg tgaaacttaa
    56761 aaaagctaaa tggggggatt tcattgagtt ggcaagaatt gtatcaaagt aaattatgta
    56821 gtgctacaga agctgtaaaa cagattaaaa acggtgatac tgtagtattt gcacattgtg
    56881 taggtgaacc tcctgcacta gtagaggcta tgattgaaaa tgctgaacaa tataaagatg
    56941 ttgagattaa acatatggta agcttgggta gtggtggata tactgctaag ggaatggaag
    57001 ctcattttag agtaaatcca atgtttgtaa gtggaaatgt aagaaaggca attgaaaatg
    57061 gagatggtga ttttacacct gcattcttcc atgaagtacc aaagttgctt agagaaaaaa
    57121 gattaaaatg tgatgttgtt ttagcacagg taactccacc agatgaacat gggtattgta
    57181 gcttaggaac atcagttgat tatacttatg aagccattaa aactgctaga acagtaattg
    57241 ttcaagtgaa tgaccagttt cctagaacct atggtgaggt agtccatgta tcagagtttg
    57301 actatatagt ggaaaaatca caacctttat ttgaactaca acctgcaaag attggagaag
    57361 tagaagaagc aattggtaaa aattgtgctt cattaattga agatggttct actttacagc
    57421 taggtattgg tgggatacca gatgcagtta tgttattttt aactgataaa aaggatttag
    57481 ggattcattc agaaatgatt tcagatggga ctttggccct ttatgaaaaa ggtgttataa
    57541 atggtaaata taaaaatttt gataaagaaa aaatgacagt taccttctta atgggtacta
    57601 aaaaattata tgactttgca aataataacc ctgcagtaga ggtaaaacca gtagactatg
    57661 tgaatcatcc agcaattata atgaaacaac ataagatggt ttctataaat tctgctatac
    57721 aagttgattt aatgggacag gtagttgcag aggctatggg acttagacaa tttagtggtg
    57781 ttggaggtca agttgacttt atacgtggag tgtctatggg ggaagatgga aaggcaatta
    57841 tagctatgcc ttcaataact acaaaaaaag atggtacagt aatttcaaaa atcgtatcta
    57901 ttgtggatga aggtgcacct ataacgacat caagaaatga tgttgactat attgtaacag
    57961 aatacggtat agcagaatta aaaggaaaat ctcttagaga aagagcaaga aatttaataa
    58021 atatagctca tccaagtgta agagaaagtc tagcagtaga atttgaaaag agatttaagg
    58081 agaaatatta ataatagagg ggtgttaata atggaagcac ttagagtagt tccaaagata
    58141 ttttattttg atacatttaa agaatttaat gaagaattta aaataggaaa aaatgattta
    58201 gtaattacta atgagtttat atatgagcca tatatgaaac ctcttggaat agatacaaat
    58261 ttaatttttc aagaaaagtt tggaacaggt gaaccatctg atgaaatgat agactccatg
    58321 acaaaggaaa tgaagaaata caattttgat agaattattg cgtttggagg aggaacaata
    58381 gttgatatat gtaagatatt agcacttgat gttccagaaa agtctattga tttatttgaa
    58441 ggagaagtat cacctaaaaa agtaaaagag ttagtagtag ttcctacaac atgtggtaca
    58501 ggtagtgaag taacaaatgt agctattgca gagttaaaat ctaaacatac taaaaagggg
    58561 cttgcagtag aagaaacata tgcagactat gcagtgttaa ttccagagac agtaaaaggg
    58621 ttaccttaca aattctttgt tacaagttcg gtagatgctc ttatacatgc tattgagtct
    58681 tatctttctc caaaagcaag tccatttaca gagatgtatt ctctacaagc aataaaaatg
    58741 attatggatg ggtataagaa gatagttgat aaaggagaag aagagagatt taatcatctt
    58801 agagactttg tacttgcatc aaattatgca ggaattgctt ttggaaatgc tggttgtgca
    58861 gcagttcatg cactatctta ttcaataggt ggagctttcc atgttgcaca tggtgaagca
    58921 aattatcagt tctttactga agtatttaag atgtattcta gaaaaaaacc taatggaaaa
    58981 attataaaat gtacaaaaat tttagcagat gcccttgaat gtgaccctaa ctgtgatgtt
    59041 tatggagaat tagaaaagtt tttaaataaa ttaatagcta aaaaagcttt gagagaatat
    59101 ggaatggtgg aaagtcagat agatgaattt acagatagta caatagcaaa tcaacaacga
    59161 ctacttgcta ataattatgt agagcttagt agagaggaaa taagagagat atttgcaaat
    59221 ctttattaat acaatttttt atttatatgt atagtcaaca taaacaatgc taattatatt
    59281 tatgtacaaa taaaaataag ggaacttaaa ttttaattaa agttaagttc tcttattttt
    59341 atatggagtt aaatataagt atatttttaa tttaaaatat gcttatattt aagtctatta
    59401 ctttatttga aacaatttgt agaatttcaa attccttgac agtaataagt aagtaattgt
    59461 ataatcaaac tgcaatatat taataatatt agtgaaaatg aaaggattat aaaatatgta
    59521 taaatcagaa aatttttcaa aaaaatctgt attaataaat tcatttttaa gtaaattttt
    59581 aaaaataatt ggatggggtt tggtaagctt atttacatta tcagccttag tactttcaac
    59641 tatttccatt ataattggat gggctgtaat acttcctatt caagtatgtg cagtattttt
    59701 acttatagga ggagtttgta tttttaaagg taagagatta ggagaccaaa tttctcgtta
    59761 taaaaaatat tgtacaatta taaacaatag aaatattatt ccagtagaac ttattgcaga
    59821 aaacacatct aaatcattaa attttataat aaaagatgta caaaagatga tagacaaaaa
    59881 ctactttata aatacttata ttgataaaag aaataatcag attgtgttaa caaatgaaga
    59941 ttttattcca ccaagatatg aagatgtagc atatgaagtc aaagaagaag aaaagactga
    60001 tgaatccaat gaagttgata caattataaa taaaggaatg aattatctaa cacaaataaa
    60061 agaagctaat aaaaatatta aatcagaatc aatgggcgat aaaattgtac aagtagaaga
    60121 tgttacaagt aaaatatttg atgttgtaaa acatgaccca tcaaaactta ctcaaataca
    60181 aaaatttatg gattattatc ttccaaccac tttaaaacta ttaaattcat atcatacatt
    60241 ggaagaacaa ggaattgata gagaaaatat aacaacaact atggaaagta ttgaaaatac
    60301 aatgaatacg attgtagtag cttttgaaaa tcaattggac tatctgtttg aagatgaagc
    60361 cattgatatt tctacagata ttactgtttt agagaatatg ttagtccaag aaggattaac
    60421 gagtggtaaa ttttaacttc aatagaaaaa atattctgtt atttttaaac aattaattag
    60481 tcataaagta taaaatgtgt attatataag ttaatcagta ctttatgaag gaagaggaga
    60541 gaaagataca atgaataatt tagatgatat accagttatg ccaactttga cacttgaccc
    60601 atttggagaa gaatcaaaca ctaatgatat agataattct gacttattaa tgaaaaagga
    60661 tgaaaaagac ccagaagaag aaaaactgtc tgaatctgaa cgcaagatgg taaaggaatt
    60721 tgcggaaaaa atagatatta caaatacaaa tatgattttg caatatggag ctggtgctca
    60781 aaaaaaggta gcaagttttt ctgaaacagc tttaaagagt gtaaaaacaa gagatttagg
    60841 agaagttgga gatatgctta caaatctagt tactgattta aaagcatttt cagcagatga
    60901 aaaagaacaa agtggttttt taggaatttt taaaaaggca aataataaaa tttcaaattt
    60961 aaaaactaag tatgatagtg cagaggtaaa tgtggataaa gtaagtaaag agcttcaaaa
    61021 gcatcaggtt aaaatattga aagatatagc aatgcttgat aagatgtatg aattgaactt
    61081 agcttactca aaagagctta ctatgtatat tatagcagga aaacaaaaat taaaggatat
    61141 gaaagaatat gaaatgccaa agttgagaga aaaagctcgt ttatctggtt caacagaaga
    61201 tgctcaatct gtaaatgata tggtttcact ttgtgacaga tttgaaaaga aaattcatga
    61261 cttagaatta actagaatgg tatcgcttca aatggctcct cagataagat tggttcaaaa
    61321 tagtaataat ctgatggcag aaaagattca atcaactata gtaaatacca ttccattatg
    61381 gaaaaatcaa atagtgcttg ctcttggaat ttctcactta aatcaagcta tgaaagcaca
    61441 gcgtgaagtt agtaatatga caaatgagct tttattgaag aatgcagaaa ctttgaagat
    61501 gggaacaata gaaacagcaa aagaatcaga aagaggaatt gtagatatag acacaattaa
    61561 gaaaactaat caatctctaa tatctactat tgatgaggta gttaaaatac aacatgatgg
    61621 tagaataaag agacaagaag cagaagttga attatcaaaa atagaaaatg aacttaaaag
    61681 taagcttctt gaatttacag ttaaataaaa attatactaa tataaaaaga gactgtctca
    61741 aaatagagat aagtctcttt ttcattgttt aaatgattaa agtatattta tattataaat
    61801 ttctattatc ttttaacata acatcatgag ctccaccttc aactatacta gtagaagata
    61861 caagtgtaag tttagcattt ttttgaagtt caggaatgct aagtgctcca cagttacaca
    61921 tagtagaacg aactttactt aaagaaagag ttacattgtc tttcaaagaa ccagcataag
    61981 gcacataaga gtcaacacct tcttcaaaag aaagtttctt atctccacca aggtcatatc
    62041 tttgccagtt tctagcacga gcagaacctt ctccccaata ctctttcata taactaccat
    62101 ttatattaac tttgttagtt ggactctcat caaaacgaga gaagtatcta ccaagcatta
    62161 taaaatctgc acccatagct aaagctaaag ttatatgatg gtcataaacg ataccaccat
    62221 cagagcaaat tggaatataa atacctgttt tttcaaagta ttcgtctcta gcttgagcaa
    62281 cttcaattat agaagtagct tgtccacgtc ctataccttt ttgttcacga gttatacata
    62341 tagaaccacc accaatacca actttaacga agtcagcacc agcttcagct aggtaaagga
    62401 atccctcgcg gtcaactaca tttccagctc ctaccttaac tgtatcacca tatttttcac
    62461 gaatgaaatc aagtgttatt ttttgccact cagaaaaacc ttctgaagaa tctatacata
    62521 aaacatcagc accagcttca actaaagcag gaacacgttc agcaaagtct ctagtattaa
    62581 taccagctcc aactacatat ctcttagaag aatctaaaag ttcaagtgga ttttctttat
    62641 gagaagagta atctttacgg aataccatat acataagatt accattatca tcaagtacag
    62701 gaagagaatt aagtttgtgg tcccatataa tgttgtttgc ttcctttaaa gtaacatctt
    62761 tgttagcata aactatagat gacataggag tcataaattc actaacttta gtatctaaat
    62821 ccatacgact tatacgataa tcacgacttg caacaatacc caataattta ccagttgaag
    62881 ttccatcttc agtaacagct actgtagaat gtccagtttt ttcttttaag tcaagtatgt
    62941 cttttaatgt gttatcaggt ttaatatttg aatcacttac aacaaaacca gctttgtggg
    63001 atttaacacg tgctaccata gcagcttcat tttcaatagt ttgagaaccg tatataaatg
    63061 aaataccacc ttctttagca agtgcaatag ccattttgtc atcagaaaca gattgcataa
    63121 tagcagaagt caaaggtata ttcatataaa tatctgcttc ctcgcccttt ttaaatttag
    63181 taacaggtgt ttttaaactt acattagcag gtatacaatc agtagaagag aaacctggta
    63241 caagaaggta ttcactgaaa gtatgtgatg gtgtatcaaa ataaaacgcc attttttaat
    63301 cactccttat atttttatac tttttataat tgatagtatt aattataact tattctttaa
    63361 ttttattaaa gtattttgtc aaatattgag atttttgaaa aatttaaact attttaatta
    63421 aatttgttta atatatattg ccttataggg taaagttgta tattaaataa tgtttagtgt
    63481 ttaatagtag ctaatttaaa attaatcttt agtgattcat ataattacat ttgtatagtt
    63541 tatattgata atattactag agagtgcata aacctcaaaa atacagataa attaaatcta
    63601 tattagtata tccaaaagta catttaataa atttatttaa ctgaattatt attttcattt
    63661 ctattaaatt taaatatttt ataaaaatag tattatttac aaaatcatat actccaaaat
    63721 actacataaa taaaattaaa caaaaactaa aatgacaaat gtcattatct aaagtgacat
    63781 agaaacggtt tcttaaagtt gttactataa aatataaact ctataataaa agtataacaa
    63841 aagggaatca tatcgatata gaatataatt caagaggagg taaaattatg agtagttcta
    63901 atcaatcgac aataaagaca tctatacagc gagtagggaa atttttaagt ggtatggtaa
    63961 tgccaaatat aggagctttt atagcatggg gtcttataac agcattattt ataccaacag
    64021 gatggatgcc taatgaaaaa ttgagtacta taggcgaccc aatgataaaa taccttttac
    64081 cactattgat agcttatact ggtggtaaag caatagcagg acaaagaggt ggcgtaatag
    64141 gtgcagcagc agctatggga gtaattgttg gtgcagatat accaatgttt atcggagcaa
    64201 tgataatggg accatttgca ggatgggtaa ttaagaagtt tgataaattt gtagatggaa
    64261 agataccaac aggatttgaa atgttagtaa ataacttttc aataggtata ataggtatgc
    64321 ttcttgcaat actaggattt tatgcaattg gaccagcaat agtagcaggg acagctctta
    64381 tagaatctgg agttcaattt atagttagca aaagtttatt gcctctagtt tcagtattta
    64441 tagaacctgg aaaagtatta ttcttaaaca atgcaataaa tcatggtata ttagggccta
    64501 taggtatagc tgaggcaaaa gaagcaggta aatctataat gttcttacta gaaagtaacc
    64561 ctggaccagg tttaggagtt ctattagcat attggatgtt tagtagagga agtgtaaaac
    64621 aatcagcacc aggagctgta ataatacatt tctttggtgg tatccatgaa atttacttcc
    64681 catatatttt aatgaatcca gttttaatat tggctacaat ggcaggtgga gcagcaggaa
    64741 tattgacttt ttcaatatta ggagctgggc ttgtagcagc accatcacca ggaagtatat
    64801 ttgcacttat ggcactagct ccaaagggag gattacttcc agtattagca ggagttgcag
    64861 tggcaacagt tgtatcattc ttagtggcag caccatttgt taagagagct tcagcaaatc
    64921 aaagtgaaga agacagtact tctttagaag aagctaaagc aaaaatgagt gatatgaaat
    64981 ctgcaagtaa gaactctgag aaaaatattg aggaaaaaca gcttgaagta aatgagatta
    65041 agaagattgt atttgcatgt gatgcaggaa tgggttcgag tgcaatggga gcttccagat
    65101 ttaaaaatag aataaaaaac ttagacttaa atatagaaat tacaaattca tctgttgata
    65161 atcttccaga tgatacacaa atagtagtaa ctcacaatac attagttgaa agagttgcta
    65221 agaataattc aagtgttgag atagtttcaa taaacaattt tttaaatgac ccaaatttag
    65281 atgcattatt taaaagatta gaatctaaat aaaaataaaa agtaccacta attaggagtg
    65341 gataatttga aaaaaaagaa gattacatct aggcaaaaaa agataatact aatgatagta
    65401 gaaaattcaa agaaaaacat tccaataact atttctgaga ttgcagggac tttagagcta
    65461 agttcaagaa ctgtacttag agatatgtct ggaattgaaa aatggtttga tgaaaatgat
    65521 tttaactttg taaaaaaacc aggagtggga ttgattttag aggaaaatat agaaaatcaa
    65581 aactttataa ttgagttgtt agaagaagaa aaaatagaaa aagaatacag caaggaagaa
    65641 agaaatttaa ttatattatc aaagctacta gtttctaacg agccagtaaa atcctattat
    65701 tttgcaaaaa tattaaaggt atctgaaggg gtactgaata atgattttgc cttagcaagt
    65761 aaatggttgg aaagatttga tattgagctt gttagaaaac cagggcttgg tgtctactta
    65821 aaaggtcaag aaaaaaactt tagagaggca tatgtaaatc ttatatacga ttcattcaat
    65881 gaaaaagaaa tactagatat ggtcagaaat ataagtgaaa atatacaaac agataaagca
    65941 atagaaattt taagtgaaaa tagactttta aatttaatgg acaggtgcat aataagaaaa
    66001 gttgaagcta cattaacaaa gaagctttca gatttagatg taaatttagc tgatagtgca
    66061 tatatagggc ttgtagttca tatttcatta gccttacaga ggataaaaaa tggtgaaaat
    66121 ataactatgg ataaagagtt tctaaaagaa ttatctataa cagaagagtt taaattagct
    66181 aaagaaatcg taaaaggtat ggaaacagat tttaacatgg atataccagt agatgaggtt
    66241 ggatatataa ctatgcatat aagaggtgca aagcaaaggt catcttcaaa ccataaggct
    66301 ttaaacttag atgatataga aataatggaa attacaaata agatgataga tttggcggaa
    66361 gatgaattta aaataagttt aaaaaatgac gagaggttat ttaaagacct agcaaatcac
    66421 ttagggcctt ctataaatag attgaatatg ggattagaaa taagaaatcc tcttttagat
    66481 gaaataaaaa gcaagtattc atatgcctat gatggagttg aaaagatttc tagaattata
    66541 aaagataaac taaatataaa ttcaatacct gaatcagaaa taggatatat tgcaatgcac
    66601 tttgcatcag ccatagaaaa aaatcttatg atgaatacta atataaatat tgttgtggca
    66661 tgtcccactg gaataggtac ttcaagattc ttatcaacta agattgaaaa taaatttcct
    66721 aatttaaata tacttgaaac catatcagct ataaacatag atgaagagta tttaaaagaa
    66781 aaagatgttg atttaatagt gtctacagtt gagttaaata caagtttaaa ttatatatgt
    66841 gtaggtccat ttatgagttt agatgatgaa cagataatca aggaaaaaat aaaatctata
    66901 gctcaaaata aattaatcaa tttaaatgcg aaaaatgata ctaaaagtaa gaataaagta
    66961 tatgaacaga taacagaaag tatgaatata ggaaaagata ttcttcaatt tttagaggaa
    67021 attagatttg aaaaatttga aagtaaagat ttaagtgagc ttattgaaga ctcatcaaaa
    67081 atatttgcaa aatctagtga agatataatc tctataaaag aatcgttaaa agaaagatta
    67141 aaaatctcaa ttccatatat agaagaatca aaaattctat tgttacattg tatgagtgaa
    67201 agaatagata ttatgaagtt atcaataata aaactagaga atagaatcgt gctagattca
    67261 aatgaagaaa tcgataatgt agtttttatg ttacttccta aaaattcacc aagttatcaa
    67321 agacaaataa tgagtgaaat aagtggttca ttaatagaca atataatttt tactaataag
    67381 ataaataaat tctctataga agagatgaca ttagaggtaa aagatatagt ttttaatttt
    67441 tatacaaaca gattaaaagc atttatagat aaataaaatt aatattaaaa aggaaggttg
    67501 atactatgag taaagtattg aatgagaata atatattttt agggttagat agtgtttcaa
    67561 aggaagaagc tataacttta gcaggaagaa aattagttga aaatggatat gtaaaagaag
    67621 aatatatacc agcaatgtta gaaagagaaa aagtaatgac tacatatatg ggaatgggag
    67681 ttgctattcc acatggtgta aatgaagcaa aaaaagaaat attatcttct ggaatagtta
    67741 tattacaatt ccctaatggt atagattttg atggagaaaa agcatattta ttaataggaa
    67801 tagctggtgt tggagatgaa catttagaaa tactatctaa tatagcagta gttttagatg
    67861 atgacttaac agaaagacta aaaaattcaa atgacaaaca agcttttatg gaagcttttg
    67921 ccaactaatc ttaggaggga tttaaaatga aaaaggcgat tcagtttgga gcaggaaata
    67981 ttggaagagg ttttattgga agcttgttag ttaagtcggg ttatcatgta gtatttgctg
    68041 atgtaaatga ggagatatta aactcaataa acaaagataa aaagtatact atacacataa
    68101 gagatgtaga atgtatagat gaagtaattg ataatataag tgcggtttca tctataaaag
    68161 aagaaataat agatgagatt gtacaggctg aaataataac tacagcagtt ggacctttag
    68221 ttttgactaa aatagcctct acaattgcaa aaggtataaa agctagaaaa gaaaagggat
    68281 taacaagtaa tctaaatata atagcatgtg aaaatgcaat atacgcaagt tcttctttaa
    68341 aagaagaggt acttaagtat ttaaataagg aagaagtaga atatttagag atgtatgttg
    68401 gttttccaaa ctgttcagtt gatagaatag ttcctcctgg taaaaacgaa aatcctcttg
    68461 atgtaacagt tgaaaatttt tatgagtgga atgttgaaaa acaaggattt aaaggggaaa
    68521 taccaactat agttggaatg aatttagctg ataaccttat ggcttatata gaacgtaaat
    68581 tgtttacact gaatacaggt catgctataa cagcttatat aggatattta aaaggatata
    68641 agactataga agagagtata aaagataaat ttatctgtga tatagtcaaa agtgcaatgg
    68701 tagaaagtgg agaaggattg attaaaaaat acaattttga ctcagaagtg cactataaat
    68761 atattgataa aattttaaat agatttaaaa atccatatct taatgatgat gtattaagag
    68821 tcggaaggga accattaaga aaactaagtg ataaagatag attgattaaa cctttaatga
    68881 cagcaaaatc atatggatta tcagttgaca atttgattct tggaataggt gcagcgcttc
    68941 attataataa tagtgaagat actcaaagtg tagaattaca agagttgata aagtctattg
    69001 gggtaaaaaa agccgttgct aaaatagcta atataagcaa tgacgaagaa attttaaata
    69061 atatcgaaaa aagttatata tttatgaaaa atttataaat ttacttgaaa aaaagtagtt
    69121 ctgataaagt aactataata tattattcta cacagtttga attatttgtt gaataataga
    69181 gaattataat atataattaa aaggaataaa ttaatatatt tacctgtata tgtttgataa
    69241 tatggttcaa atgtttctac taagctacca ttaatagctt gactacagga gtgaagaaga
    69301 tatagtttat acttatttta aattatatat taagttaatt actttgaatt ttaagctcct
    69361 gtctatgata tagacaggag cttttttagt attttaatat aaaatactta caagaataga
    69421 tagcaaaatt agaacaaata taattttttt attaatataa ttttagaaag ggctaggtgg
    69481 aagttcaatg aaggctttac aagaaaagat attgagagaa ggcagtgttt caggaaatga
    69541 tatactaaaa gttgatagtt ttttaaacca tcaaattgat gtagcttttt taaatgaaat
    69601 aggaagagaa tttaaagaga gatttaaagg agaaaaggtg gataagatat ttacaataga
    69661 ggcttcagga atagctatag catcaattgt ttcacagtat tttgataatg cacctgtagt
    69721 atttgcaaaa aaatcagaat ctaaaaattt ggataaagat gtatatgaaa ctaatgtata
    69781 ttcatttact aaggcaagag agtattcagt taaagtttct aaaaagtaca taaacaaagg
    69841 cgaaaatata ttgatagtgg atgatttttt ggcaaatgga agagctgctc ttggacttaa
    69901 agatttaatt gagcaagcag aagctaactt agttggagta ggaattgtca ttgaaaaagg
    69961 atttcaagct ggaggagcac ttttaaaggc taatgatgta agattagagt ctttggcagt
    70021 agttgaatct atagataatg gaactgtaaa atttaggtaa tttatgatat tatataattg
    70081 tgtttagcac atattttaac tatgtaatta tggaggattt tttatgaggt tttttataga
    70141 cacagccaat attgaagaaa taaaagaagc aaatgattta ggtgtaatat gtggtgtaac
    70201 tactaatccg tctctaatag ctaaagaagg tagagatttt atagaggtag ttaaagaaat
    70261 aagtgaaata gtagatgggc caataagcgc agaagtaata agtttagaac ataaaggtat
    70321 gatagaggaa gcagaaaaat tatctaagat acataaaaat atagttataa aaatacctat
    70381 gacagcagaa ggtctaaaag cggtgaaagt actatcaagt aaaggtataa agactaatgt
    70441 aacgcttata ttttctgctg gacaagctct tttagctgct agagctggag ctacatatgt
    70501 aagtcctttt gttggtagat tggatgatat atcacaaaat ggtcttgatt taattgaaga
    70561 aatcgttgat atatttagtg taaatagtat tgaggctcaa ataatagttg ctagtgttag
    70621 aaatccaata catgttttac aagctgctag aatgggagca gatatagcaa cagttcctct
    70681 taaagttata aaccaaatga ttaagcatcc tttgacagac aaaggaatag atagctttat
    70741 gaaggattgg gaaggtgctt ttttaaaatt ataattataa actacgcttt ataagtatat
    70801 taaagaggtt attatggaaa ttctaagtaa taaggctagg ctattttgcc tagtcttatt
    70861 ttggtgtata atatattgaa acatattatt gaaatagatt aattttttaa gtgaggaggc
    70921 gaaaaatgga taaaatatta agagagatac aaagagaaga taaaaaaaat ccatatactg
    70981 accaagaact tgctgaaatg ttaaatgtag ctaggtctga agtgatatca gttaggaaaa
    71041 aaaataatat tttagattcg agagaaagaa gaaaaaaaat attaataaaa gatatatcaa
    71101 aaattgtaag tgaaaatcct caaatgtctg aaagaaaaat aactgaaata ttaatagaaa
    71161 atggatataa tatatcgaga agtgcagttt caaaattatt aaaagaggaa aatttaaatc
    71221 agtcaaagat tgggattaag gataaagacc cagcatcatc aggaaagtat gtgaaggtga
    71281 gtaaagttgt agaagaagat ggatttgaag aattgatagg tataaaggga agtttgaaag
    71341 aaaaggtaaa tctagctaag tcagcaataa tgtatcctcc taatggcctt catactataa
    71401 tatatggaga aacaggagta ggtaaaagtg aattagctac atgtatgtat aaatatgcta
    71461 taaaaaacaa cattaaagaa gagaacagcc catttatagt gtttaactgt gcagactatg
    71521 cagaaaatcc taatctattg atagctcaac tctttggggt agtaaaaggt gcatatacag
    71581 gagcagatgc aaatagagaa ggtcttgtag aacaagctaa taatggaata ttgtttttag
    71641 atgaaattca tagattgcct gctgctgggc aagaaatctt attttcatta atagatagag
    71701 gagaatttag aaggttagga gaaagtagtt ctaatagaaa ggcaaatgtt ctcataataa
    71761 gtgctacaac agaaaatcca gattctaatt tacttcaaac atttagaaga cgtataccaa
    71821 tggtaataaa tttacctaat atgagtgaaa gaccaaaatc tgaaagatat gaaataataa
    71881 taaagttttt tgaaagagaa gcaaagcgag taaacaagaa ttttataata actaaagaag
    71941 ttatgtgtgc acttatgaat tataagtgta ctggaaatat aggtcagtta aagagtgata
    72001 tacaagtaac ttgtgctaga gcttttagta aatcgatatt ttctagcgat aaggtaatta
    72061 tagatttaga tagtttaagg gattatataa aaagtggata ttatgattgt aattgtaaaa
    72121 atgaaaagta ttctgagttt tcagttgagg atatatatat agatattagt gaattggtga
    72181 gtggaaaaaa tagaatttta tctgataatg agataagcga gatatataat tatgcagaaa
    72241 aagagcttaa ggttttggaa agtaagtgct tttcagaaga agagttaaaa aatgcattta
    72301 tagaaaagtt agataagaag tttaatgcta ttaaaaacaa taaaaattta tctgatagaa
    72361 gaagtcgaat agactgggga ggacaattaa aagagtctac actggagata atggataagg
    72421 taattgtatt tattaaggag cagtttaagc atgtaaatca aggtctgtac ttggctctag
    72481 caattcatat tgagcatgct ataggtagga taaaagatgg aaaaactata ataaatccga
    72541 gtttagataa aataaaagca tcaatgccaa tggaatatga gttgtcaaga tatataatgg
    72601 gtatagtaga agatttgaca caagtaacat ttccagaaga tgagcttgga tatttggctt
    72661 attatataaa taaattttgt tataatgagg aaagtataaa agataaagtt aaagtagtaa
    72721 tagttacgca tggtaaagtt ggaattgaaa tgtctaaagt tgtaaatcat attttaggta
    72781 tagaatgtac actaggtata gaaatagcac ttacagattc accatcagag gggatagagc
    72841 atgtattaga agagcttcaa aagatagaag ctagaaaagg tattctagtt ctaattgata
    72901 tgggttcttt ggtaattctt ggtgatgagg ttgaaaaaag gcttggaatt agatgtaaaa
    72961 ctgtaaatag agtagataca ttattggcaa tggaagcagg aaaacttgca accattgaag
    73021 gaaaaagttt agatggtata atagctgatt taaagaaaaa taagaattat gctatgataa
    73081 atacaaataa atttagttac agaaaaaacg agtatggaaa gaaaaatgta atcattactt
    73141 tatgtttgag tggtgtaggt acagctttaa atttaaaaga gcatatagaa aaacaaatta
    73201 aggaatatgg cactttaatt gaagtaaagc cagttgcttt tttaaataat aatttagaag
    73261 aagagttaaa tgatattgaa gctgaatata atattattgc tatctgtgga actattgata
    73321 tagattataa aaacgtgcct tttatttctt ataatgaggt acttggtaaa aatggtatta
    73381 ataaagtatt agagcatcta aatagtaaac aaccagcttt aaatattgaa aaccagttga
    73441 ataatttaat acatgaagat ttgattttat acaattttga aggaatttca aaggagtata
    73501 taattgatac tcttgtttca aaacttgaag aaggtggata tgttgattca aagtatatat
    73561 taagtgtata taaaagagaa gcaatgggaa gtgttgtaat ggcctctaag gtagccgtgc
    73621 cacatggact tccagaaaac gttataaagc ctgcaatagc aatcgcaagg ttgaataaac
    73681 ctatagtatg ggacaataaa tttatggtgg atttggtggt actactggca cttaaagaaa
    73741 ataataagaa agaaatacga agcttatttt caaagattaa tgaccagcat actttagaag
    73801 ttttattaaa tactgaagat aaaaatgaaa taaaagaact tttaagctaa ttattagtac
    73861 cgagaagaaa gacaaaaaaa tttgctttag tataaaattt aaaattactc aattgctaag
    73921 tagttaatta ctctatataa ggctagttct atgaaattta tttatatgat tttatagaat
    73981 tagctttttt cttggtaaat aaaatatatg aatttattta tgtaaaaagt aaattt
//
LOCUS       DAMKPG010000006        72239 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMKPG010000006 DAMKPG010000000
VERSION     DAMKPG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 72239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.006"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(121..894)
                     /locus_tag="PC621_000461"
     CDS             complement(121..894)
                     /locus_tag="PC621_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF4107651.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC621_000462"
     CDS             complement(894..1280)
                     /locus_tag="PC621_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4107652.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC621_000463"
     CDS             complement(1305..1622)
                     /locus_tag="PC621_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF4107653.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC621_000464"
     CDS             complement(1634..1894)
                     /locus_tag="PC621_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF4107654.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC621_000465"
     CDS             complement(1894..2190)
                     /locus_tag="PC621_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF4107655.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC621_000466"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC621_000466"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF4107656.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC621_000467"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC621_000467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF4107657.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC621_000468"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC621_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF4107658.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC621_000469"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="PC621_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF4107659.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="PC621_000470"
     CDS             complement(8083..9189)
                     /locus_tag="PC621_000470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF4107660.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC621_000471"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="PC621_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF4107661.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="PC621_000472"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="PC621_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF4107662.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="PC621_000473"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="PC621_000473"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF4107663.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="PC621_000474"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="PC621_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF4107664.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="PC621_000475"
     CDS             12283..12990
                     /locus_tag="PC621_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF4107665.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="PC621_000476"
     CDS             12959..13588
                     /locus_tag="PC621_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF4107666.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="PC621_000477"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="PC621_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF4107667.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="PC621_000478"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="PC621_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF4107668.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="PC621_000479"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="PC621_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF4107669.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="PC621_000480"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="PC621_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF4107670.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="PC621_000481"
     CDS             18876..19649
                     /locus_tag="PC621_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF4107671.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="PC621_000482"
     CDS             19651..20514
                     /locus_tag="PC621_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF4107672.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="PC621_000483"
     CDS             20547..21689
                     /locus_tag="PC621_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF4107673.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="PC621_000484"
     CDS             complement(21823..22398)
                     /locus_tag="PC621_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF4107674.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="PC621_000485"
     CDS             22658..23950
                     /locus_tag="PC621_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF4107675.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="PC621_000486"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="PC621_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF4107676.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="PC621_000487"
     CDS             24730..24993
                     /locus_tag="PC621_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF4107677.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="PC621_000488"
     CDS             25008..25193
                     /locus_tag="PC621_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF4107678.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="PC621_000489"
     CDS             25296..26492
                     /locus_tag="PC621_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF4107679.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="PC621_000490"
     CDS             26706..26984
                     /locus_tag="PC621_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF4107680.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="PC621_000491"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="PC621_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF4107681.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /locus_tag="PC621_000492"
     CDS             27474..27701
                     /locus_tag="PC621_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF4107682.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="PC621_000493"
     CDS             27844..28164
                     /locus_tag="PC621_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF4107683.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="PC621_000494"
     CDS             28172..29128
                     /locus_tag="PC621_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF4107684.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="PC621_000495"
     CDS             29139..31133
                     /locus_tag="PC621_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF4107685.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="PC621_000496"
     CDS             31134..31583
                     /locus_tag="PC621_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF4107686.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="PC621_000497"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="PC621_000497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF4107687.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="PC621_000498"
     CDS             33135..33443
                     /locus_tag="PC621_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107688.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="PC621_000499"
     CDS             33525..34814
                     /locus_tag="PC621_000499"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF4107689.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="PC621_000500"
     tRNA            35140..35215
                     /locus_tag="PC621_000500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="PC621_000501"
     tRNA            35222..35296
                     /locus_tag="PC621_000501"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="PC621_000502"
     tRNA            35301..35376
                     /locus_tag="PC621_000502"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="PC621_000503"
     tRNA            35410..35484
                     /locus_tag="PC621_000503"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
     gene            complement(35867..36859)
                     /locus_tag="PC621_000504"
     CDS             complement(35867..36859)
                     /locus_tag="PC621_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4107690.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36868..37926)
                     /locus_tag="PC621_000505"
     CDS             complement(36868..37926)
                     /locus_tag="PC621_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF4107691.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38051..39514
                     /locus_tag="PC621_000506"
     CDS             38051..39514
                     /locus_tag="PC621_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF4107692.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39616..40413
                     /locus_tag="PC621_000507"
     CDS             39616..40413
                     /locus_tag="PC621_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4107693.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40426..42528
                     /locus_tag="PC621_000508"
     CDS             40426..42528
                     /locus_tag="PC621_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF4107694.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42596..43075
                     /gene="rlmH"
                     /locus_tag="PC621_000509"
     CDS             42596..43075
                     /gene="rlmH"
                     /locus_tag="PC621_000509"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF4107695.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43224..43535
                     /locus_tag="PC621_000510"
     CDS             43224..43535
                     /locus_tag="PC621_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4107696.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43583..43900
                     /locus_tag="PC621_000511"
     CDS             43583..43900
                     /locus_tag="PC621_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4107697.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44113..44814
                     /locus_tag="PC621_000512"
     CDS             44113..44814
                     /locus_tag="PC621_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4107698.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45038..46339
                     /locus_tag="PC621_000513"
     CDS             45038..46339
                     /locus_tag="PC621_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4107699.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46476..47570
                     /locus_tag="PC621_000514"
     CDS             46476..47570
                     /locus_tag="PC621_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF4107700.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47572..48705
                     /locus_tag="PC621_000515"
     CDS             47572..48705
                     /locus_tag="PC621_000515"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF4107701.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            48723..49832
                     /locus_tag="PC621_000516"
     CDS             48723..49832
                     /locus_tag="PC621_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF4107702.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            49897..50637
                     /locus_tag="PC621_000517"
     CDS             49897..50637
                     /locus_tag="PC621_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF4107703.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            50642..51634
                     /locus_tag="PC621_000518"
     CDS             50642..51634
                     /locus_tag="PC621_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF4107704.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51821..52702)
                     /locus_tag="PC621_000519"
     CDS             complement(51821..52702)
                     /locus_tag="PC621_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4107705.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52975..54363
                     /locus_tag="PC621_000520"
     CDS             52975..54363
                     /locus_tag="PC621_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107706.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54391..55344
                     /locus_tag="PC621_000521"
     CDS             54391..55344
                     /locus_tag="PC621_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF4107707.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55404..55628
                     /locus_tag="PC621_000522"
     CDS             55404..55628
                     /locus_tag="PC621_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107708.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55621..56133
                     /locus_tag="PC621_000523"
     CDS             55621..56133
                     /locus_tag="PC621_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF4107709.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            56165..57019
                     /locus_tag="PC621_000524"
     CDS             56165..57019
                     /locus_tag="PC621_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF4107710.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57586..59673
                     /locus_tag="PC621_000525"
     CDS             57586..59673
                     /locus_tag="PC621_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4107711.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            59688..59975
                     /locus_tag="PC621_000526"
     CDS             59688..59975
                     /locus_tag="PC621_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4107712.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            60002..61273
                     /locus_tag="PC621_000527"
     CDS             60002..61273
                     /locus_tag="PC621_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF4107713.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            61331..61930
                     /locus_tag="PC621_000528"
     CDS             61331..61930
                     /locus_tag="PC621_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF4107714.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            61935..62840
                     /gene="dapA"
                     /locus_tag="PC621_000529"
     CDS             61935..62840
                     /gene="dapA"
                     /locus_tag="PC621_000529"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF4107715.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            62946..63578
                     /locus_tag="PC621_000530"
     CDS             62946..63578
                     /locus_tag="PC621_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF4107716.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
     gene            63993..66164
                     /locus_tag="PC621_000531"
     CDS             63993..66164
                     /locus_tag="PC621_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF4107717.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            66169..66786
                     /locus_tag="PC621_000532"
     CDS             66169..66786
                     /locus_tag="PC621_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4107718.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            66975..67319
                     /locus_tag="PC621_000533"
     CDS             66975..67319
                     /locus_tag="PC621_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF4107719.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            67889..68755
                     /locus_tag="PC621_000534"
     CDS             67889..68755
                     /locus_tag="PC621_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF4107720.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            68868..69272
                     /locus_tag="PC621_000535"
     CDS             68868..69272
                     /locus_tag="PC621_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF4107721.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            69450..69899
                     /locus_tag="PC621_000536"
     CDS             69450..69899
                     /locus_tag="PC621_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4107722.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(70055..70432)
                     /locus_tag="PC621_000537"
     CDS             complement(70055..70432)
                     /locus_tag="PC621_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4107723.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            70694..71611
                     /locus_tag="PC621_000538"
     CDS             70694..71611
                     /locus_tag="PC621_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF4107724.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIETVKEFWE"
     gene            72094..>72239
                     /locus_tag="PC621_000539"
     CDS             72094..>72239
                     /locus_tag="PC621_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF4107725.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGI"
BASE COUNT        27527 a         8632 c        12007 g        24073 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaatcattct agcatagtat tatgtaatat agcaaattta atttatagta tcaataaata
    35701 tagactacta agaatgtaaa tgatttgtta gattaagata cattagatat gtaaatagaa
    35761 aaataaaaaa tatgttttaa tttatgtggc ataagtttta aacagagaag caagtttgct
    35821 cttacctctc tatttaaaaa taaaaaaatg ttttttaagg gctattttat ttaaatcttt
    35881 cccatctcaa atcatctcca gtaaattcta aaatcctaaa cttatctaag attctagaga
    35941 gagttctttg agtgtaagta tttcctattt gagatggtgt taagttggtt gaaataatga
    36001 tttttttacc agcaacaagt cttgtattta caatattgaa gatttcccca ctagtaaaag
    36061 aattattaag ttcagttcca aggtcatcta ttattaataa atcacaatca aacaagtttt
    36121 tataattatc ttcacttatt tggttatttg tgtctcttct aaatttataa tcttcaagaa
    36181 tatctaaaat tctaaaagaa gtttgataga ttacaacatt tcctttatct aacaattcct
    36241 ttgcaataca attgcacata taggtctttc ctagtccagt agaaccataa aacagtagat
    36301 tttcactatt atccttctta aaatcatgaa caaaattttc gcagatactt aaattattta
    36361 gcatattttc tctaggagat atttccccat cagagccttt cttaggagaa aatatattca
    36421 agttaaaatt cgaaaaattt tcctgactta aaatcctatc taaattagac attttataag
    36481 cttcatttac aatttcttgt ttgaggcaag aacatttttc tccattaggt aaaaatcctt
    36541 tatctttaca agaaatacat tgaaacttta tttccaagta atcaagaggt atgttacttt
    36601 cagataaaag tttttctttc tttactttta aagactctat attctcttta gtcttcttta
    36661 caatttcatc tttagatttg ggatttaaaa gtactatctt agctaaactg agacctattt
    36721 tagaaatttc atcatctata gattttatct cagggattct attatatacc tcattttttc
    36781 tatgttctaa taagagttca ttatcatctc ttctcttagc atatttagca agtatttttc
    36841 ttattttatc ctcattcatt taatcaccta cttaaacttt tctttttgac tttttttaat
    36901 aatctcatcg agctcatcag aggtatactg agtgaatgtt tcgttgaaat tatgaaactt
    36961 agtttttttg taagtattgt tgttttgagt agtattaatc tgcttcttat taatattctc
    37021 cttaactata cgttcttcct ctttttgctt taaatcattt aaatttttta tatttttttc
    37081 attccaattt ttaattatac catttatata agatatactt gggtttgaaa tattcttaga
    37141 cttagaacat gcatttatta tcaaatctat atccatatta aatttatcaa accaggtgtc
    37201 cataagttct ttttctgatt ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt
    37261 tttatataaa atatatcgtt cacttcttac caaaaagctt tcttcaacat cttttggcgt
    37321 atataaattt gaatcatacc aatttctaat aatgccttca atgtatttaa ctggctttga
    37381 tgtaccagtt ttatctttga cgtattcata tgcgcaaagt atcatatcgg ggctcatatt
    37441 gtatttattc attatatcca taatactcat cttttcactt gggtcgagat atcgcccaac
    37501 tattttattt atagaattaa acatttttct aatacttgga ttttcagaag tagatacaac
    37561 cttatctgta ttagatttta tagatgaatt attgcttaat atattctcaa tatagaaatt
    37621 ttttaaatct aaaaattcta ttgaataatt aaaattatca tattctccat tgtcatgtat
    37681 ttttataatc ttcttttctt cccagaattt ccaagcagat aacacatcag acaatggtat
    37741 atttaggttt ttggctattg aattattgtc gaacttagga ttagaagtaa tatcacatgc
    37801 ttgtctatat cctaaaagat aaacttttac gtataacccg tccgccattg gcataaatat
    37861 atctataaag atattgggaa tagtgatttc acctaaatct atttcattgc tttctttaaa
    37921 aaacatttta tcacctcaga ttaaattata tcatttttta tattatattt gtctgtaaac
    37981 aaataaaagg tcacaagatt agcatgtaaa tattttctaa aagtattcat tttgaaaagg
    38041 aggagatatt ttggttataa aattcaacca aaaagtaaga agaacattat cagtagcgat
    38101 tctatttatg gtattaataa taggttgctt tatagaaaag gatgacagtt tagaaacata
    38161 caaagaaaaa actaattcaa aaataaatca gtataatatg gatgttatat ttgatgatga
    38221 gactaagagg ttaatgtgca atcagaatgt tgactacata aataatacaa aatcaaacat
    38281 agataaaata tatttccata tatatccgaa tgctttttcc aagaaagatt ttgctccttt
    38341 tgaaaaaagt gaaatgacaa gggcttatcc taatggattt aacgaaggat atatagatat
    38401 aaagaatata ttaaataata atagtaaaat ggagtataaa attaaaggag acaaaaatga
    38461 catattagaa atagaattag ggaaagagtt aaaaccaaat gaaagaatgt cattagatat
    38521 aaaatataac gtaaaaatac caaattcaat tggaagattt ggatatggag aaaatactat
    38581 aaatgttact aattggtttc ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa
    38641 gagttatgaa actattggcg acccatttta tagtgagact agcgattttc atgtgaaact
    38701 attgattcca aataaatata aaattgctca tacaggaaaa cttattgatg ataaacatga
    38761 taataagaaa atgctatatg aaatagaagc tgaaaacgtt agagattttg catttatact
    38821 aagtagtaaa tttgatgtaa acaaagtaga ttcaaaggga gtagctatca atacatataa
    38881 tttaaataaa aagctttcta aaaaagcaac tgaagtggca aaagattcaa taaatatatt
    38941 tagtgaatta tttggaaagt atccttacaa agaattttct gttgtagcaa gtgacttttt
    39001 tatagggggg atggaatacc ctatgcttgt tatgatagac caaagtttat ataatgaaaa
    39061 aaatgaattt ttactagaat atgttattgc ccatgagaca gcacatcaat ggtggtattc
    39121 tgctgttgga aatgatgaaa taagtgaacc ctggttagat gaagcactta ctgaatattc
    39181 tacaatagtt tattttgaac aaaagtatgg taaagatatg gcaaacaagc ttataaaaac
    39241 tatggagata cagacaaaaa gttatttgag tgaaaatata tttaagccag caaatgagta
    39301 taaaaattct actgaataca gcttgaatgt ttatacaaaa ggcgcaatag catttaatga
    39361 aataagaaaa gaagttggag ataaagtatt ctttgagact ttaaatgaat actataataa
    39421 atataagcat aaaaatgcta atggaagagc ttttgtggaa ctatggaata gtaaaggtgt
    39481 agatataaat aagattataa gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc
    39541 agagtattaa attttattta atactctgtt tttttggtat aatataattt ataaggcaga
    39601 aaaagaggtg aaggaatgct aaagtattgt tcagtaggga gtggtagtag tgggaattgc
    39661 cactatatag ggtataaaaa tactaatata ctagtagatg caggattaag tggcaagaga
    39721 ataactacag gtcttaaaga cattggtgta gatgcagata aattaaaagg aatatttata
    39781 acacatgagc atgttgacca tataaaagga gcaggtatat tatccagaaa gttcgatata
    39841 ccagtatttg ctaatataaa aacatggtgt tctatgaagg acaaattagg tgacatcaaa
    39901 gataaaaata tgaaagtatt tgaaaatgat aaaacctact cattaggtga tatcatagtg
    39961 agaccttttt caatagaaca tgatgcgtct gatccagttg gatataattt ttatacagag
    40021 agtgatgaaa aaatgtctat agctactgat ataggatgta taactcaaaa tataaaaaaa
    40081 catctatata aatctaaatt agtagtattg gaatcaaatt atgaccctaa tatgcttatg
    40141 atgggttcat atacatatgc tttaaagaaa agagtaatgt caagtactgg tcatttatcc
    40201 aatgaagatg cagctaattt ctgcgttgaa cttattaatg aaggtacaga atctatatta
    40261 ttagcacatt taagtaaaga aaataatttt cctgagttag catatgagac ttcaaaagga
    40321 gtattagcat caaatgatat agtagttgga caagatgtaa aactagatgt actatcacga
    40381 aatgatgttt ctaatgttta tgagatgaag tagaggtgaa taactttgct aaagagaaat
    40441 tttgaaaatg ttgaggataa taaaatttta tactctataa agcagggtat gattttagca
    40501 atacctgcta ttatgactgg ttcaacggct cttgtaattt tgaatcttcc aataaaggct
    40561 tatcaagaat atttatcagg cttgtttaat ggagaggtta caaatattct taactttata
    40621 aatgattcta ctttaggaat tatttcatta ataatattat taacaataag ctatagttat
    40681 ggaaaaatat atgggagcaa gtatacagta cttgttccta tagttgcaat gtgctctttt
    40741 ttagtatttt cacatggtaa tgagcttaat tcttatatag aaatatttaa gacaaagtgg
    40801 ttatttacat ctataattgt ttctatgaca tcttctgtat tgtttgtaaa acttacagaa
    40861 tcttttgtta caagtgtaaa gtttcataca gaaggggctg atgcagactt taatatggta
    40921 gtttcggcta tagttccttt cataatagta gtatttttgt tttctatttt tagagtgata
    40981 atgataagtc taattggcag ttcaaacttt caagatatat tttataattt attttcaaat
    41041 gtttttaata aaatgggaac aaaccttatg agtgctctgt tgtttatttt tttaatgcac
    41101 tttatgtggt tttttgggat tcatggtagt aatgtattag ataccgtcgc aaaaaattta
    41161 tttgaaaata gtatggcaat taacataaat ttagttaata ataatcaatt accaacagaa
    41221 atttttacta agaccttttt tgatacaatg gttttattgg gaggatgtgg ctctttacta
    41281 tgtttagtaa ttgcaatttt tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa
    41341 attgcttcaa taccagctct ttttaatatc aatgagatgc tagttttcgg gataccaatt
    41401 gtttttaact atattatgtt tgtacctttt gtaattactc ctataatctt aaccattaca
    41461 agttatgtgg ctatgtctac aggtattgta ccatgtacag tttcagctgt agaatggacc
    41521 tcacctatat ttttaagtgg atatatgtct acaggttcaa taagaggtag tttgttgcaa
    41581 gtatttaatt tatgtgttgg tgttatgatt tatattccgt ttataaaaat gtcgcaaaat
    41641 agatatacat atatgtttaa gaataatata gaaaatttaa caaacttatt aaaaaggtat
    41701 gaattaacag gtgaacagcc taatcttcta aaccataatg gtaaaatagg ttcaatttca
    41761 aaaatgttgg catcagactt gaagtttgct atgaaaagag gagaaataga acttttttat
    41821 caaccacaag tcaattataa tggaaatgtt gtgggagcag agggattact tagatggaaa
    41881 cattcaatag gagggttcat ttatccacca ctagttataa ttttggctaa agaagaaggg
    41941 ttcttagatg agcttggaga gtatattatt aataaagcat gtattgattt aaagaagagt
    42001 gaaaagctat tgagaaatcc tgttaagttc tctgtaaata tatctccaga ccaacttgat
    42061 gaccctaagt taccggagca aataaaaaat atcattagta gtaatgatat aaatcctaat
    42121 atgttaggta tagaaataac agagcaaatt gcattatcag gttcacaaat aattattgaa
    42181 cgtatcaatg cgatacataa cttgggaata aagttaatta tggatgattt tgggatgggg
    42241 catagctctc ttatatatct tcaaaataat aactttgata tagttaaatt ggatggttca
    42301 ttagttaaag agatacttac aaataaaaga agtagtgaaa ttataatgac tattgtaaat
    42361 ctttcaaaaa atttagattt tgatatagtt gttgagtatg ttgaaaatat agaccaaagg
    42421 gataagttat atggtttagg atgtgaaata tatcaaggat attattatag caaagcaata
    42481 ccttttgaag agtttgtaga atatgcaaat caagaaaaag agatataatt ttataagagc
    42541 ttgtaaattc tagtaatgca tagatttgta ataaaattaa gtttaggaga aatatatgaa
    42601 tataagtata gtagtagtag gtaaaattaa agaaaaatat ttaaaattag gtattgatga
    42661 gttcaaaaaa aggctttcta agtattgtaa gttagaaatt atagagttgg atgatgaaaa
    42721 agctccagag aatttgagtg ttaaggaaat ggaaattata aaagacaaag aaggaaagaa
    42781 aattttaggg aagataaagc ataatagtta tgttatagtc ttggctattg atggtaaggg
    42841 tttatcctct gaagaacttg ctaagactat gagtgattta gcagttagag gtaatagtag
    42901 tctctgtttt ataataggag gttctttagg gctttctgat gaggttttag gaagggctga
    42961 ttataggtta tccttttcta agatgacttt tcctcatcag atgatgagat tgatattgtt
    43021 agaacagatt tatagggctt ataggataaa taatggagaa ccgtatcata aataaatgat
    43081 acggtttttg ctaatttgac acttaataat attaaaataa caaaaaagta ttgaaagtat
    43141 attgcattta aggtagaatt aaataaaata ttataacatt ataatgttta tatagttgtt
    43201 aataaaaatt tggaggaaaa tatatgataa aaataatgtt ggcctgttca gcaggaatgt
    43261 ctacaagttt attagttact aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc
    43321 aaatttgggc aataccagaa tcaactatac aaaatgaaat agaaaaatgt gatgtattat
    43381 tactaggacc acaagttaga tatgtattac ctaaggctca agaaattgct aagccatata
    43441 atataccagt agaggttata aatatgatgc actatggaac agttaatgga gaagctgttt
    43501 taaatagagc gatagaactt aacaacacta agtaatataa attataatgt atttttaata
    43561 tataaattgg gagaatcaaa ggatggatga aaagattata gaaatatctt ttaatataat
    43621 aggttatgca ggtgaaggta aagggttagc ttttgaagca ataaaagagg ctaagaatgg
    43681 aaatatagaa aaagcaaaag aattattaaa agagtctaag gaagttataa ataaagcaca
    43741 tagatatcaa acagaattaa tacaaaacga agctagtgga aacaggactg aaataagtgt
    43801 catacttata catgcacagg accacctaat gaatggtatg aattttcaac agttagctga
    43861 agaaatcgtt gatttacatt tgaaattaca agataaataa aaattaaaga gaataaatta
    43921 agagagttct taaaagatac attatgtgta gcacactaat tgttagatta ttgattcaat
    43981 atttagtgag taatacatta ggattatttt gaggactctt tttatattta gtttaaagtt
    44041 aataaatatt tattattaat aatataagat tatatgttat aatattataa catataattt
    44101 aagaggtgaa tcatgttaag aaatcaacca ttatatatac aagttgtaga tatattaaag
    44161 caaaaaatat taaataacga atataaaatt gatgaaaata ttccaacaga aagagaatta
    44221 gagcaagaat ttggagtcag taaaattact attagaaaag ctattgagat attagaatca
    44281 gaagggtatg tagagaaaaa aagtggaaaa gggacaaaag taataagtaa tagtcttttt
    44341 aataagctat caaaagcaga gtctttttca agtatacttt tatctaaagg tcataagtta
    44401 tctaaaaaga ctatttcaat tgaaaaaata ataaatgata caaattcaca agtatttagt
    44461 atatttggag aaaaatgtag caagattact agaatgtatt acttagatag taagccatat
    44521 atatttttta ctcattatat tccttttgat gaaaatctaa ctgttgaaag ttttaataat
    44581 gaaagttctt tatatatgta cttatatagg agaaatataa atataactag atttgaagat
    44641 gaatttttta tagaagaatc agaactagag gtatcaaagg ctctaaatct agctaaagac
    44701 atattattag gaagaaaaag aaagacctat gatgagaatg ataatatagt tgagctatca
    44761 tttgcaagat ataatacaga agtacaaaac tacgtaatta aatacgaaat ataataagaa
    44821 tcctgagttt attgaaatga taatatgctc aggatttttg tttagttgta aaaacgaaaa
    44881 atattataac attataatgt ttacaaccaa acattataat gttataatat ttttaataaa
    44941 taagtgatat tatcaattac ataaaaatta gtttatgtta tgaaatcgtt attgcattat
    45001 aaataaagga taatatcaat aatacagggg gataactatg gaaaaattcg caagtttttt
    45061 agagcgcaag attatgcctg tagcagcgaa gataagtaat cagagacata tgagagctgt
    45121 aagagcaggt ataatagcaa cattaccact aacaatagtg gggtcatttt tcacaatatt
    45181 attaaacata cctattgatg cttatatgga ttttatagct ccatttaaag caacattaga
    45241 tataccattt agatatactg taggattttt atctctatat gctacttttg ggatagcatc
    45301 atcacttgct aaaagctacg atttagatac aacaggtgtt ggtatgcttg cagttatggc
    45361 atttttagtt tctacaatag ttccaactca agtagttgaa ccagtaggtg gagttataga
    45421 agctggtaga tggatggata tggcaaaact taattcgcca tcattatttg gagctatcgt
    45481 aacatcatta attacagttg aaatatatcg ttttatgaag gaaaagaata ttacaattaa
    45541 gatgccagca ggagttccag tagaggtttc aaattcattt actgcattat taccgacagc
    45601 agtaatttta atgttattct gggtaataag acatatttta ggatttgata taagtgttgc
    45661 actaagcgct atgttatcac ctttaaaagg attcttatca ggaaatagtt taggtggtgg
    45721 gctactgaca gtattcctaa taactttatt ctgggtactt gggatacatg gtccagctat
    45781 aatggggcca gtaataagac ctatatggga catgtctata gcagaaaata taactgcatt
    45841 cacagaagga gtatcagctc atgcaatgcc aaacatattt acagaacaat ttttacagtg
    45901 gtatgtatgg atagggggag caggggctac tcttgcatta gttgtaatgt ttatgttttc
    45961 taagtcacaa tatctaaaaa gtttaggtag attaggattt ttaccagggt tattcaacat
    46021 aaatgaacct ataatatttg gagcaccaat agttatgaat ccaatactag ggataccatt
    46081 tataattgga cctataataa tgacaattct atcatatgtt cttactatta caggggttat
    46141 acctatgatg gtagcaagat taccatttgc aatgccagca ccaatagcag cagtaatgag
    46201 tactaactgg agtatactag caggtatctt agtattaatc aactttgtga tatcatttgc
    46261 tgtttattat ccattcttca aagtttttga aaaacaacaa ttacaaagag aacaagaaga
    46321 aatgagtgaa aatttataaa aatacataga tatagaggtt ttgtactata taaaatatag
    46381 aaaaagctta taagttatta agggtgaagg taggaggaat gacctttgcc tttaatttta
    46441 tatattttaa ataatgtaat ttgaggagga gaaagatgga aagaagatta ggaatatcag
    46501 tatatccaga gcatagtaac atggaagatg ataaggcata tataaaaatg gtttcaaaat
    46561 acggatttaa aagaatattt atgtgcatgc tttcaattac aagacctaag gaagaagtta
    46621 aaaaacattt taaagagatt ataacctatg caagagaatt aggatttgaa gtaatccttg
    46681 atatagctcc aaacatattt gatatattac aaatatctta tgatgattta acattcttta
    46741 gtgagttaaa tgctagtgga ataaggcttg actatggata tgatggaagt aaagaagcta
    46801 tgatttcttt caatccatat gatttaaaag tggaactaaa tatgagtaat gatgtagcat
    46861 acttggataa tataattact tacaagccaa atgtaaataa tatatatggt tgtcacaatt
    46921 tttatcctca aaggggtaca ggcttgccat atgatttttt tgtaagatgc tcaaatagat
    46981 ttaaaaagta tggaataaga actgctgctt ttgtcaattc aaaatacgca acaatcggac
    47041 cttggaatat aaatgatgga ctgtgtacgc ttgaaatgca tagaaactta gatattgcta
    47101 cagcaactaa gcacttattc gcaacaggat tgattgatga tgtaattgta ggaaatgcat
    47161 atgcaagtga agatgaatta aaaagaatgt ctgaagtaaa tgaataccaa gttgtattta
    47221 atgtagtagc taaagaaaaa acttctgaag tagaaaagat aataatggta aatgagcaac
    47281 actttagaag aggagatata actaatcagg tagcaagatc tacagaagta agaaaaaaat
    47341 atgctaaaga aaatttccct aaacatgata ataagaacat ctttaaaaga ggagatgtag
    47401 tgattggtaa tgatgacttt ggaaagtata aaggggaatt acagttgata cttcaagatt
    47461 gtacagatga aagaaaaaat aaggtgggta gaataaagga agacgaaata atacttttag
    47521 actttattga accttggagt aaatttaaga ttattttaga gggagagtaa agtgaagata
    47581 gacatattaa taaaatgtgg aaaaacagta gataaaagta taattgatat agctatatta
    47641 gacgataaaa taatagaagt aaaaaatcat atagatgatt ctaagtatca ggcaaaacaa
    47701 attataaact tagatggaga gaaatttatt agtgcgggtt ggatagatat acatacacac
    47761 tgctacgaaa gactagacct ttataaggat tatccagatg aggttggaat aaaaagtgga
    47821 gttactactg tagtagatgc aggaacaaca ggagctttag atataggtga attttatgaa
    47881 gatactaaaa agtataagac caatgtatat gctttaataa atattgctaa gcagggaata
    47941 acatctcaag atgaactttc aaatatgatg aatattgatg aatatgaact taaaaatgca
    48001 gttagaaaat acaaagattt tgttgttgga atcaaagcta gaatgagtaa aagtgttgta
    48061 atttcaaatg atgttgaacc tttaaaagta gctaaaagaa taaaaaatga acttaatcta
    48121 cctatgatgg ttcactttgg gagttctcct ccaacgatag aggatatatt tgattatatg
    48181 gaaaaaggag atatattaac ccatatatat aatggaaagc caaatggaat acttagaggc
    48241 aatgaggtta aaaaggaaat catagaagct aaagaaagag gtataattct tgatgtaggt
    48301 catggaaccg aaagttttag tatggatatt gcaatgaaat ctaaggatgc aggaattttt
    48361 ccagacacta taagtacaga tatatatatt aaaaatagaa taaatggtcc agtgtataat
    48421 ctatctacaa caatggaaaa gtttatatat atgggctatt cacttgaaga tataatagat
    48481 aaagttacta aaaatgcagc agatgcaata agtttaaaaa ataaaggttt aataaaagaa
    48541 ggatatgatg ctgatttaac tatatttgat gtggttaatg aagaaaaaga acttcaagat
    48601 tctttgaata agtcagtaat cacaagtacg tctataaagc caagagcagt agtagtcaat
    48661 ggagaatatt taaatatagg caactctata gaagagaatg agtagaaggg agaaactatt
    48721 gtatgagtag tatatttgaa aaatactctg taagacgtgt tataaatgct tcaggcaaaa
    48781 tgactatact aggagtatca acagtagata aggaagttat tgaagcaata ggtgaagggt
    48841 gtaaaaattt ccttataata gaagaacttg taaataaaac aggtgaatat atagcaaatt
    48901 tattagggtg tgaaagtgca ctaatagttt ctagtgcatc tgctgggatt gcacagagtg
    48961 tgggagcaat gatagcaaaa gatgatatga gtctagttta caatgtaaat aatcctaaga
    49021 aacaagttaa aagggaaata ataattccaa aggggcataa tgttgattat ggagttccag
    49081 tagaagtaat gataagactt ggaggaggag aagttgttga ggccggctat gctaatatgt
    49141 gtaacaagga acatataata agagagataa atgaaaatac agcagcaatt atgtatatta
    49201 aatctcatca ttgtgttcaa aagagtatgc taagtgtatt agatgcagta caagttgcta
    49261 aagaatataa tttacctttg atagtagatg cagcagcaga agaagattta aatgtatatt
    49321 atgaattggg agcagaccta gtgatataca gtggtgctaa ggctatagaa ggtcctagct
    49381 cggggttagt aataggtaag aaaaaataca tagataatgt aaagcttcaa tcaaagggta
    49441 taggaagagc tatgaaaata ggaaaagaaa atattgtagg tctaacatgt gcaattgaga
    49501 gatatataag taatgataaa gtaactttaa aagaaatgga ggacaagcta aatccattta
    49561 tagacaagat aaatactata aagggagtga gtgctagtat aactagagat tcagcaggta
    49621 gagaaatact tcgtggagaa attaacttta atgaagatat aataaataag tcaacacaac
    49681 aaattataaa tgaacttaga agtggagaga tagctatata cactagagac tataaggcta
    49741 atgaaggcaa gattgagatt gatataagaa gtgtaactgg aagtgattta gataatattt
    49801 ataataggat aaaaaatata gtagaaagtt agagtgtaga gtaagtgaca ttataatgat
    49861 ttaataaatt gaattttacc aatggagggg ataaatatga attgtatacc acaatattat
    49921 aaaaatagag tttgtttaaa tgttcttgca ggaagcataa aaaatgctaa agaagtatat
    49981 gatgcctgtg atggacatgt actagtagga gtactatcaa agaattatag cactgtagaa
    50041 gcagctattg aagatatgaa aaaatactca aaggaaatag ataatgcaat atctgtgggt
    50101 cttggagctg gagacccaaa tcagtcaaat atggtatctc agatttctaa agaacttcag
    50161 ccacaacata taaatcaagt gtttacaggt gtcgctacat ctagagcatt gcttggtcaa
    50221 aatgatagta taataaatgg attagtttct ccaactggta aaccaggatt tgtaaaaata
    50281 tccacaggtc cattaagttc aaaagaaaaa gatggtatag ttcctataga aacagcaata
    50341 gctatgatga aagatatggg aggaagctca attaagtttt ttccaatggg agggcttaaa
    50401 acaaaggatg aatatataga agttgcaaaa gcttgtgcaa agcataattt ttatttagaa
    50461 ccaacagggg gtatagactt agataacttc aaagagatag ttcaaattgc acttgatgct
    50521 ggtgtagaaa aagttatacc tcatgtttat acatcaataa ttgataaaga aacaggtgat
    50581 acgaaagtgg aagatatagg agtactttat aaaataatga aagaattatt gggatagatt
    50641 tatgaatata ttagcttttg gtgaaataat gatgagactt tcagtacctg actacaagtt
    50701 cttaacacaa accaatgaac taaattatat aattacaggc acgggattaa atatattatc
    50761 tggacttaaa aactttggat acaataccta tatgttgaca aagttgccta ataataatgt
    50821 aggtaaagca tcaagtgcaa atataagaaa gcttggagta aaagatgact ttatcacata
    50881 tggagggaat catataggag tgtattttct tgaaaatggg tatggagaaa gaccttctga
    50941 agtgacttat ttgaataggt taaatagttc attttgtgag agtaaattac aagattatga
    51001 tattgataaa tgtttagaag aaatggatat agtccatatt tgtggtattg ctatgcaact
    51061 tacagattca gttaaaaaga ttgctttaga actagctaga aaatcttatg aaaaggggat
    51121 aaaagtattc tttgatttta attttagaca atcattaaat ccagaaagga cttatgatga
    51181 tttagtaaat gattataaga aaatactacc atattgtcat gggctgtttg ctagttatag
    51241 agatataaat aagatactta atatagatag tctaaatata aaatctagtt cagaattgat
    51301 taaaaaagag tatgatatag atattgtagc tggaactatg agaagatttg atgatatgaa
    51361 taatagattt ataaagggat atatatcaaa tgatgatggt tattttgaaa gtagggagta
    51421 taagttagaa atatatgaca gagttggtgc tggggatgga tatgtggcag gaattatata
    51481 ttcatatttg gaaaatatga agagtgaaga tatagttgag tttggggttg ctagttcggt
    51541 gttagctcat acaacttatg gggataattc tttagtaagc acaggtcatg tacttagact
    51601 gatgaacaat gaaaaaatag atatattgag ataatacatt tgtatataat aaaattttta
    51661 tcttttttag acacactttt gacttgctta tatttgttaa acagagttat ttttaacgat
    51721 tataaatatt aaattaaaag ttagataata aaatagtaga aaatagcatt aaccatataa
    51781 aaatggttaa tgctattttt atagttaata ttgattaatt ttatattata gaaatgaact
    51841 tttctaatgc tggatttgta ttttttttat tccatacaac cacaacttca attctatttt
    51901 ctacttcttt tataggtact attgcaatat cacgttctat cattgtatct gtaacacaaa
    51961 atgaaggtaa aatggatatt cccatgttag aagataccaa tgattcaata gtattaagat
    52021 tatttgaacg ttgtaccaca ttaggtacaa accccgcttc acgatatcta ttttgtatca
    52081 ttgacttttg tccatactca tcccctgttt cttttacaaa aataaattta tcatgttgta
    52141 attcaattaa atcgatagaa tcctcattgt aaagtggatg accaggaggt aaaagagcta
    52201 ccaaagaata tctttctaca gttttatatt caaaatcttt taatggttga ttttctggat
    52261 taataacaaa agcgaggtcc atagaattat tttttactaa gtttaaaagt tcggttattt
    52321 cattagtaca aatatccata gatatattag ggtatgtatt agaaaattct tttaagtatt
    52381 gttgaagttt gtttttttca tatcctatta aggttccaat gctcaaagtt ccaaaaaaac
    52441 catgtgcaaa ttgttgtgtt tttgcaatag catcattgat attttttatt atagtacgtg
    52501 cctcagataa aaaaactttt ccggctggtg ttagttcaac atgacgttta cttcttgtaa
    52561 atagagttac atttatttgt tcttctaaag cttttatttg ctgtgtaaca gcagtttgag
    52621 aaatataaaa tttttctgct gttttggtaa aacttagatt ttctgctaca gatataaaat
    52681 attctaattg tttagtattc aaataatcac ccctatttaa acttgattgt ttgtaaacct
    52741 tatttatcta taattaaagc ttattatagt ataagaaaat ttacttttaa agaatattag
    52801 aaaatatttg tataaaaata tatgttttaa gtatatcttt acattagatt ttataaattt
    52861 attaataaga ataacttata tattccaatg aaaacgatat tggatttaag taagtttgtc
    52921 tcataaaatg aaagtatatc aattgataat actttaaata agaagggaga aaaaatggaa
    52981 gataaatttt atgcaaaagg caacggaaat aacggatata ttaaaaatct tgaagtttgt
    53041 tcctttaata acttagatgg aacttgtgga atgtttcaaa tggctctgta caaaagagat
    53101 gaaaaatact atttatatgg atgctgtttt ggaggaaata aaaaaaatgg agtaatgatt
    53161 agcgatatta cagaccctta taatccacaa tttataaaac attttcaaat gttagaccct
    53221 aaagagtatc ctacaacaac aactccaaaa attcaaatag cagatgattt aatgatagta
    53281 gcaatgagtt gtggaagtgg accaggagca cttgttgacc aagctaaatt agcaaatatt
    53341 aagtgtgaag caggaattag aatatacagt ttaaaagaag accctttaaa tcctaagttt
    53401 ttaggatatt gggattgtgg cttaaagcat gtaatgggtg ttcatagatt catgtacaat
    53461 ggtggaagat atgtacattt atcaagtgat tgtgttggct ttgaaggtct gatttatagg
    53521 gtcatagata taataaatcc taataatcca gtggaaatag gtaaatggtg gagaccagac
    53581 caatatgcag atggatatcc aaatagaact tttgatgcag gagcacctca ttgcccagaa
    53641 tttatggata aaggatggct tcatggacct ccatttgtaa gagacggaaa agcatattgt
    53701 ggttatggag gagctggttt agttgtatta gatgttgaag atttaacaag accaagatgc
    53761 ttaggtgaat tgccatttat gcctgcattt tctagtagac ttgcaggtgc aagaactcat
    53821 acagcattac cattgccagg aagagattta gtcgttgttc aaaatgaggg agaaagattc
    53881 cagttcttta aaccagataa cattacagat gttcaagcta tgaataatat acatatggtt
    53941 gatgttagtg acccaacaaa accaacatta attgctcaat tcccatatcc tgaagttcca
    54001 aaagatttcc cttatcctaa ctttaatgtt gcaggattag gaaaagcagg gccatttggt
    54061 ccacataatc ttcatgaacc aatggataat aagccatggt tagagcaaag aggagataga
    54121 gtatattgct gttatttcca tgcagggcta agggtttatg atgtatcaga cccatattat
    54181 atcaaagagc tagcatattt tataccacca aatccaaata aaacaccaga agaatcttat
    54241 ttcccaggat tcccaggacc acgcttggca gtaacagaag atcttatcgt tgatgataga
    54301 ggctacatca tcatagatgc tttagatgat ggattctata tattaaaaat gaaagaagat
    54361 taaataaata ctaaagaggg agagttgaaa atgaaaatac taggtatttc ttttggaaca
    54421 aaaaatggaa ataatgacac aatgtgtaaa gttgcactta aaggtgccaa agaagcaggg
    54481 gctgaagtag aatttattca aatgtcatct attaacatta aacattgtac tggatgttgt
    54541 gcgtgcgtta aaactttatt atccgggaaa ggtagtatgt gtgtattaaa agatgatttt
    54601 gaatggttgc ttgacaaaat gaaagatgca gatggtatag tagtttcaga ccctattttt
    54661 gaagaagggg catcaggatt attccatact ataatggacc gttttggacc cagagcagat
    54721 agaggaaata acattatagg aacaaaagtt gcagaagcta taggaggaaa aattccagac
    54781 tcaagaatgc ttaatgataa agtaatatca tttatgggaa taggaggttc tgactggggt
    54841 acaagagttc aatgtgaaca tgctatgcta gctctcatac caatgtggaa agttatagat
    54901 aatgcttggt tcccatgggc aaaggaatta gtaatggatg atacaaggct tgttcaagtt
    54961 catcaaattg gtttaaatat agttgaagca gcaaaagatt ttgaaaaagc ttcatatcaa
    55021 ggagaagaag gtgtatgtcc acattgccac aatagattat tctaccttga gtcaggtact
    55081 aaaaaggcaa tttgtgctct atgtggtata gttggagagt tagatactat aagtggaaaa
    55141 actatattta gttttccaga agagcaatta ggtcatgctc atgatacttt accaggtaag
    55201 tttatacatg gtgatgatat taagaaaatg gaaggtcatt atgcagaagt tcgtaaaaca
    55261 caagaattta aagaaagaaa aacatcttat aattttatag agccattaac taaagaaaag
    55321 caagctggag tttcattagg ataataatta aaatactaag actctttttt aagggatgtt
    55381 agatgttata ggaggcttat tgtatgaatg aaaagccaag ttttaaacaa attgaaatta
    55441 ttttagcagt tatttttttg gtacttacat attacaagat tggattaact ccagcattta
    55501 ttgttatatt tataccatta tacttaagtt attttgcagt atctatgatt gtaaatcaaa
    55561 tagcaattaa gctattggag ataaggcaag ctagggaagc tactaattac aatataggaa
    55621 atggatagaa agaaaaaaga aatattaaca ctgtctatgg ggattgcttt actacctcct
    55681 ttatgggcag tattagcacc ttatatagga ataaaaactg gagctgtagc actaatctgt
    55741 gcaggattat atgtaactaa tggtaataaa caaaaagatg gattaaaaat aatgtttggt
    55801 ttttggtgtg gagatttatg ggcagtgtta gccattttaa ttatgggata tatgaatttc
    55861 aatcaaaacc tagaattatt tttaacactt agtatattag gtttttttgc agttgtaatt
    55921 gcatccttat ttgaaaaaat aattttccta ccatcatggc tttgtggttg ggctataggt
    55981 ctaactatta tgacaggtga aaatattatt aatttacaag atatttgtat tcaaattgct
    56041 gtagctatgg cagtaggagt atggtacgtg ggtgcaggtg tagatttgtt tacaatatat
    56101 attttgagta aagataagaa aaagggaaat tgaaagcatg tgaatatact ttgattatag
    56161 gagaatggct atgggttttg aatttaaaat aatgagaagt ttaatatatg taggacttgc
    56221 caaggaagaa tatagaccta agttaatgga ctggttatat cgtcaccata ttccagatag
    56281 tattagcact tttggaccat attgtactaa atatgccttt tatcaagcat atcctacacc
    56341 aaatgaaggt gagcgttttg gtgcacgtaa gatgcaacta acagaacatt attggcttgt
    56401 agatgaacat atgcctgaga tggcaaatag aattatgaca gaatatatgc ctatggatgt
    56461 tctacgttgg caagggtgta taccagatgt agaaaataaa agggttcatg aaaatgcaga
    56521 aagtggagat gcaggacgtg cagtaggtgg agataatgga tgcccaccat ttatatttgc
    56581 ttttgttcca ataaactggg aagaagactt tagaggaaaa ggacgtactg tacaagatgg
    56641 accaaactat cgttggcaat ttatgattaa gtatccagat ggtatctcta aagaagaagg
    56701 agaaaaatgg ttctatgatg aggtagtgcc atactttaca aactgttgct atgttaatcg
    56761 ttttgtcagt agtaaaataa tgattaatta tggagcaact gcttttgacc gtgtatcaga
    56821 actatggttt gaaggggaag aggaatggta taaagctgtg gttgaagaga caaagtcgtt
    56881 tattaaaaaa ccagaatggg cacaagaaga ggagttccca tatttaaaac cacaattcaa
    56941 tatcgcatca gtattcttag gtgatatagc aactatggat gcatactcac agtatcgtgg
    57001 atatatacca atgagataaa aattatagtt tgtgataagt agaggaagta cttactctta
    57061 ctgctagaag aagacttatt gtacacttaa aagaatgaaa tagataagtg tcaatttatt
    57121 atatgagaga aactttaact ttcttggttt aaaatattaa agtcataagg aacatttatt
    57181 attaaatact ttatttatat gaattaattg aagaaaaagg atattaaact acaagttaaa
    57241 aaataaattt aatatattag aattataaat aaggcacatt aattttattt taaattaatg
    57301 tgccttttat ttatggtaat ttatgcataa ttagtatcgc tttgattctt taaataaatt
    57361 ttttttggtt tctttacata aggaaccctt ttttatttat aaaatctata agtgtaatat
    57421 agtagctatt ttagataagt ttaatttttg tctcaatcta aaacaaaagt tatacttaaa
    57481 tgaaattgaa aacgataaaa ttctcaaata aaataagtat tgtggaggtg atgaaagaat
    57541 ccaatgataa tatgaagact aatcttaata agaaagtagg ggttaatggt agtggacaaa
    57601 gaaattgtat caattctcca gattttatta aatagcacat tgattacaac taatggatta
    57661 caagaggagt ctaattcatc aaagagacaa attacttata gaataaataa aatcaatgac
    57721 atgcttaaag taaaaaatgt accacttata tgtttaagag ctgataaaga tattatcgtt
    57781 agaaaagaaa caaaaaaagc aattaaagaa attttagaaa aaaactattc taagaatact
    57841 tattatttta gtaagacaga aagactttta tatatgtatt taatgctttt tattaacatg
    57901 gaggatattt caattaatca ttttataaat tctataaaag taagtaggtc tactgtaaat
    57961 cttgatttca aggatttgat accagagctt gaaaagaaga acattaaagt taagaacaat
    58021 cgtataaatg gatattacat agttggcaat gagatggaaa ttaggagagt gcttattaaa
    58081 aacattatag aaactttgtc taatgagaaa agttcaagag tatttgatat ttttattaaa
    58141 gaatataatt tagatagttt tgaagaggct aaaaaaatta tattaaagct aataaaaaaa
    58201 tatgaaatta cttttgttga agatagatta atagaattta tatatatatt tatctttcta
    58261 aaagcaagga tgtattcaac tagaatagtt actcaggaaa atgtagatat tcctaatatt
    58321 tcagttatga gttcaatgaa ggagtataaa tttgctaagg aattattaga agtatatggg
    58381 agtgaggata agataaagcc ttataatata atgtatatta gtgcttggat tttaggtata
    58441 tcggtaggaa attatgaaga agatacagaa gataaggatg taatttcaaa aattgtaaat
    58501 aaaatgatga caaagtttgg atatttaagc ggagtttact atataagcca agaaaaaata
    58561 tttaaacaat tatattctca ttttagacct gcctactata gacttttatt taaattacct
    58621 atctataatc cgatttgtga aaaagtaaaa gaagaatata aattagttta tagaattgta
    58681 agtaatgcaa tgaaagagtt ttgcggatta tttggagaag aaattcagga agaagagtta
    58741 gcatatctta caatgcactt tgcaacactt ttttcaaata aaaaagaatt tgatggtttt
    58801 agaaaaagaa tagcattaat agtttgttca aatggcgttg gtagttcagc tattttacat
    58861 gcagaactta caaacttatt tcctgactta cactttatac caatgtcgga atcagttgga
    58921 ttagaaaata taagtgaacc tgtagatatc gtatttacta ctaattataa cgcagagggt
    58981 ttaaaaacag atgtaccagt aattaaagta agccctgtta tgaccacaaa agaaaaatat
    59041 aagattacac gtgaagtgta tattcaatta ggtgatattt atttaagaca acctaaaata
    59101 gatgaggtta tggatataat aaaaaaatat gcaaatgtaa cctctgaaag tattctttca
    59161 agtgagctat tagcttattt tacacagata gagaatttta catctaagga aggagagggg
    59221 cctatgctaa gtgagattac taatgaaaca ctagtaaaaa taaaggtaaa agctaagaat
    59281 tgggaggaag ctattagaca gtcagcttct gtattagttg aaaataacaa agtaactgaa
    59341 gggtatgtag atgctatgat taactcagca aaagcatctg gaccatacat agtaattaca
    59401 aaacatgttg cacttccaca tgctagacca gaagctgggt caaaagagat tgcaataggc
    59461 attgctactt tagatagtcc tattgagttt gggaataacg aaaacgaccc tgtaaaatat
    59521 gtattttgtc taagtgcaat agacaataat agccatttac gtgctatgtc tgagctagtt
    59581 gaactattgg aagaagatga gttttttaag gttttagata gtgctaaaga ggctaaagaa
    59641 attattgatt ttataaaaac acacgaatta taaaggagga tttaattatg tataaaattt
    59701 taatcgcatg tagagcagga gttggctcaa gtttaatgtt aaaaataaag actcaacaaa
    59761 ttattaagga aaataacttt ccaattgaag tagaacatgg ttcattggat tcactaaatg
    59821 gattcaatgg agatgctgta gaaacattaa ttgatgttgc tgaagaatta aaaaataaaa
    59881 atttgaagtt tgatataatt gggattcaca atattgttga ccgtaatgaa ataaagacag
    59941 cactagaaaa attccttgct tcaaaagagg gataaattta atatgataag gaggaatatt
    60001 tatggagttt attattagtt tgctgagtac acctgctgtt ttgcttggcg tagttgcatg
    60061 tataggattg attcttcaaa agaaatcagc cgttgaagta tttacagggt cttcaaaaac
    60121 acttatcggt tttttgatat ttggtattgg tgcctctgct atgacaggag cacttcaaaa
    60181 tttcaataaa ctgttccaac atggctttgg aattactggt gttatagctt caccagaagc
    60241 agctacagca cttgcacaag gtacatatgg ttttgcagtt tcatgtacat taattcttgg
    60301 attcatatta aatttagtat ttgcacgtat tacaaagttt aagaatattt tctttacaac
    60361 aggtcatagt ttattctttt cttgtgtatt agttttaatc atgaaagcac atggatttga
    60421 taatacaatc actattttaa taggtggaac tatcttaggg tttatgtctg ctgcactacc
    60481 tcaattctgt caaccattta tgcgtgaatt aacaggtggc gacgaacaag ctattggtca
    60541 ctttaacatg attggttatg gtttatcagg ttatattggg cgtttatttt caaaacataa
    60601 agatgataca acagagacta tagaatttcc taagtggtta tcaatattca gagacttctt
    60661 aatgggtcta tccatagtta tgttaatctt attctatatt gcaacattaa aagctggtca
    60721 agcatttact caagaaattg ctggttcaac acattggttg gtttttccgc ttatagaggc
    60781 atttacattt gtggcaggta tgtcaatatt gatgtcaggt gttcgtatgt tcttagctga
    60841 aataactgca gcatttgtaa tcatttcaga aaagtatatt ccaggttcac gtcctgcgct
    60901 agatgttcca acagtatttc catatgcacc aaatgcagtt attattgggt ttatatcagc
    60961 atatgctgct ggtctattag caattgctat tatggcatca tttcctagta tatttcctgt
    61021 agtaattatt ccagctgctc acatatgttt cttctcagga ggtacagctg caatatttgg
    61081 caatacttca ggaggatgga gaggggctat tgctgggtca tttgttgttg ggttattact
    61141 tgcattttta ccagttatac tatatccagt ttatgggact cttggtatag aaggagcaac
    61201 tttccctaat attgattaca acgtagttgg ttcgatatta cataatattt tacaatttat
    61261 aaaacctatt taaactaaat tatataaaag tatagtcata gttactatga tttcaaggag
    61321 gatacatctt atggaaaata cattatctga atttttagat aatatatcta atgaattgtc
    61381 agaatttatt gcctcaatag atgagacatc tataaataaa gcatgcgaat taattcttga
    61441 agctgaaaaa aatcacggac gtgtacatgt gactggtatt ggtaaaccag gacatgtatc
    61501 aggatatatt tcatcattgc tttcatctac tggaacatca gcatatatat tgcatggtac
    61561 agaagcagta catgggtcaa gtggtcaagt agttgaagga gatgttgtta ttgcaatatc
    61621 aaatagtggt gaaactcaag agttaaaagc aactcttaag actctaaaag taaatggagc
    61681 aaaaataatt ggtgtaagtg gtaatgagag ttcattttta aaaaatattt ctgatatatt
    61741 cctgttcgca ggcgttaaac aggaaggaga ttgtcttaat aaagctcctc gtgcttcaat
    61801 tttagctgaa acaatagtat tacaatcatt aagtgttgta ttacaatatg ctaaaggtct
    61861 taatacacag cagtatttga aatggcatcc agcaggaagt cttggaaagt ctattagaga
    61921 gggaatataa ttttatgaaa tataaaggga ttatatgtgc aatgataaca ccatttgatg
    61981 aaaagcaaaa tattaattca caagcaacat gtaaattaat tgattatttg attgaaaagg
    62041 ggatttatgg attatttatt ttgggtacta atggtgaatg tcatgtgtta acagatgatg
    62101 aaaaagttga atttgcaaag attgttatta atcatacaaa taatcgtgta cctgtttttg
    62161 ttggaacagg tggtaattca actcgtgaag ttatagattt atccaagaga atggaagcga
    62221 ttggagcaag tgcattatct attattacac catactttgt tacaccaaca caacaggaat
    62281 taatcttaca ttacaaaact attgctaatt cagttaattt acctattata atgtataata
    62341 tgccaggtaa gactggaatt aatattgaac cagagtcagt acgtgaatta tcaaagataa
    62401 agaatattgt tggaattaag gatagtagtg gtaaacttga caacatgaaa gcttatattg
    62461 aagtaacgca gaatgagaat tttagtgtgt tttcaggctc agattcactt attctggata
    62521 gtctaaaagc tggtagtcaa ggagctgttg cagcaacaac aaacttcctc acagagattg
    62581 gtatttctat ctatgaaaac tttatgaaag gggatttgga agaagctcaa aaagctcaaa
    62641 gtagtataga agaacttcga cgtatactaa aacttggaac aattccatct gttatgaaga
    62701 aaactattgt cttgaatgga attaatgttg gaactgcacg acttcctgta actgaaccaa
    62761 ctggggaagt acttgaagaa attaaacgag tagttgaaaa ttataggaca atacttaata
    62821 aaagtagtga gaatagataa tataaatagg gaaattacac tggtatataa ttaatatata
    62881 tattataaaa catgactaaa attaatgaat aagtaaagtg gtttgatgag tatagaggtg
    62941 ataatatgaa aaagatgaat ataaccaaaa gaatgtcaga atgtggggct cttgcaattg
    63001 taagagaaga aaatcttaac cgtgcatgtg aaattgctga aggatgtata aaaggtggta
    63061 taacagttat agaaatgagt tataccctaa ataatgctgg agaaataatt caagggctta
    63121 ataaaaagta tggtgaaata ctttgtgtgg gagcaggaac agttttggat agtgaaacag
    63181 ctcgtcatgc tatattacat ggagctcaat ttattattgc accaaattac aatgaaggtg
    63241 tagcgaagat ttgcaataga tatcagattc cttatgctcc aggatgtaca tcattgactg
    63301 aggcagtaga tgcactaagt ttaggagcga cttttataaa agctttccca atatcagatt
    63361 tctatggacc taaactagta aaggtattta agacaccaat acctgatatg ccagttttag
    63421 caagtggagg aattaatctt gaaaacctac atatatggtt agaaaatgga gttgatgtat
    63481 gtggatttgg tggtttatta actaaaggaa ctattgagga tattactgaa aatgccaaaa
    63541 agattcgtga aatcattaaa aatataagag aaatttaaca tggaggatga attagttttc
    63601 tattgacgag atatccacac attaattcct aagttagaag aaatattttc tagttgaggt
    63661 aaagtttata ttgaacttaa attaaatttt gttttaaata agatattatt ttagttgatt
    63721 gctaaaaata ctagaatcta tattaatgaa ttatactaat tttgtaccac tattaaaata
    63781 ttgtttgcta aaaataaaaa ataaccttaa aacagttgat tataaaaaat taactgtttt
    63841 aaggttattt ttatctttgt gacgaaatag acacttgcca tttagaatgt gacaatatag
    63901 tagttgttta gaaaaggtac aaagcattat actttaacta aagtaaaata tattttgctt
    63961 ttagaataaa aacaattagg agggagctat aattgaaaaa gataatatat gtattattta
    64021 caattgaact attaatagta tctttattag gtctaaatat agttaaagat aatgaaatta
    64081 ataatatttt atataacgat acaacaagta tatctgtaat gtttaaagat tataaaaagt
    64141 taaataaaaa ttatagtaag tggattaaag atattgcaga tgaaaataat gtggctatat
    64201 caaagtatgt ttttaataat aatgaaaaat tatctatata tacgactgat acaagtttga
    64261 ataacaatat aaagttaaaa tcaggaaaat ttcctaatac taattcaaat gaatttatta
    64321 gtaatataaa cactaataat agtaataata gtaatcaagt aggtaaattc tcaactatga
    64381 gtaaagacat atcaatttta attagggatt ttggtaaaat aagtgaagtt ggagaatcag
    64441 gaatcttata tatttcaact caaaatgaag atacaataaa taaaatatta agagaactaa
    64501 atgtagacag aagtattttt gtagcaagat tacatgatag atatgttaac tcaaatttat
    64561 acttaaatcc ttcaataatt agagatttaa tactaattat cctttgtttc ttggctacaa
    64621 taatacatta ttcaattagt gtgagtagag aggcttctat tttaagatta aatggatact
    64681 ctaagctaga tataattgct agggttataa aaaatatatt tagaattatg attttatcat
    64741 ctgtcacagc attattaata tatataatct atattttaaa attaaatata ggtgttagag
    64801 catgcttata ctttgctatg ttttcgatta tatgtatttt gataaatatt ttgatatcta
    64861 tattaatagt tggttttaat agtagaagtt ctaaatatgt gctatctcta aatggaaaaa
    64921 aatcttatgg ttttgtaaat ataatgcatt tcacattgaa aattgtattt gtatcatttc
    64981 ttatattatc cataaataac tgtattttaa attataatat gctacaaact cagttaggaa
    65041 atctatctga gtgggataaa gcaaaaaatg tttataatct tactcttaaa gatacaggcg
    65101 aacaatcttt aggaaaggaa gaagtaatta aaaatgccaa gattaaaggt ttctataaaa
    65161 atttggtaga agaaaaagat gctttcttaa tagattcaac taattttgaa aaacttgaag
    65221 atggaagtta catgtatgaa ataaattcaa agggtaaaaa cccagaagta agtcaatatg
    65281 gtaaaaatat aaaaataaat aaaaactatc ttaaagttaa ccctatttac tcaaatggca
    65341 aagaaatatt taatctaatc gattatgacg ataagactat aaatttatta gttcctaaaa
    65401 agttacaagt acatgaaaat gagattaaaa aagagtttag agaattattt tattttgaaa
    65461 aaatagaagt agaaaatatg tacaataaag agttaaatag agagttaaat aaaacaaaga
    65521 agtcagattt aaatgttaat ataatatatg tagataataa tcaaagctat tttacttata
    65581 actcccttgt tatggatgac aataggaatc tcattgatga cccaatagca ataattgaca
    65641 tagataatat tgatgattct ttttatcttt cttatatatc tagatgtgta tattttaatt
    65701 ctaagaaatt agatgccctt gcagatataa gtaacattat tgaagctcaa ggtgtagaag
    65761 cgcatatcca aagtttacat gctgtttata atgagtatgg tttagaaata aataaactag
    65821 aaaaatattt gaatagtgaa atttttacta taataataat agcaatatca aacttaatga
    65881 ttacctacaa tatagtagct agctactatg aaagaaataa atataagtta tatattaaaa
    65941 aaatatttgg ttattcaact acagctagaa gtatgttgtt aatagtctca ttaatattaa
    66001 caaacattat tccaataggc attatatcaa caagagtaga tttaagtaat aatattatct
    66061 tatttggact tctgatatta gttatagaag taattgtatc tattgtatta gacaagatta
    66121 tctcaaatag ttcatttaat aaaattataa aaggagaaca ttaaaaagat gatttcatta
    66181 ataaatatat ctaaaaaatt taaagataaa tctataatag acaaatttaa cctagaaatt
    66241 tctaagggcg agtttgtagc gataacagga aatagtggtt caggtaagac aactttactt
    66301 aatataatgg gattattaga aaaacctaat tctggtgata ttataataaa taatataaaa
    66361 aatccaaata gtaaacagat aagaatttta caaagggatt tttatggcta tctatttcaa
    66421 aattatgctt taattgagaa tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat
    66481 caaaaaaata tcaaccataa tgaaaaaata aattacgcac ttgagtctgt aggtcttaga
    66541 aaatacaata aaaaaaagat atacgaattg agtggaggag aacaacaaag ggtagcatta
    66601 gcaagagtaa tattaaaaga atgtgaagta atatttgcag atgaacctac aggtaattta
    66661 gataaaaaaa atagagacat agtatttgat atacttaaaa atctaaataa gaatggtaaa
    66721 actatcgtat ttgtaactca tgatttagag ttatcggagc aggcagatag agttataaaa
    66781 ttgtaataat atcaactata ataaatataa gaaagagatt acagctaaat caagtttaat
    66841 aataatacga atagaattta aacattaagt aaattagtag tttgtaaata taagtataat
    66901 atgatatcat aaaggctcaa aagaaatttt atataataag aaatttaaaa ataacaaatg
    66961 gaggacatat aaagatggaa aatttaccaa attgcccgaa atgtaattca gaatatactt
    67021 atgaagatgg aactctttta gtttgtccag aatgctctca tgagtgggca ttaggattgg
    67081 gagctgaaga agattcaaat attatcaaag atgtaaatgg aaatgtatta aatgatggag
    67141 attctgttac agtaattaga gaccttaaag taaaaggaag ttcatcatct ataaagattg
    67201 gaacaaaagt gaaaaatatc cgtttaatcc atgactcatc agatggacat gatatagaat
    67261 gtaagataga tggttttgga gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat
    67321 attataaata agctgtagtg tttattgttt ttcagtacag tataaaacca taagaatcta
    67381 ttaagtaata aaagtttgtt tatttatctt tatattttct tatggtttta ttaaatataa
    67441 attttattga acaccaaaat attctctcct ctacaaataa gccaatctta tttaaattaa
    67501 tatactttgt actatttata ctgaaattgt gaatttaaat gctatgttat ttaggtttgt
    67561 atagttaatt tatatatttc tgattttata aattgtattt attattatat cttgattcta
    67621 aaatcattaa aaatttcaac ttctataaag atttacaatt tatcaaatac attatgagta
    67681 tataaacaat ttattattac agtgctacat ttaaaataag tcttagtata aaggtattgt
    67741 tacaagtata aagaaactta tgtatgctat tggaataaat aataccttaa attataatta
    67801 agaataagaa ctgttaaata agggacttga ttatcattta gttatagata atataatatt
    67861 aaaaataaag cttaagagga gaaaaaatat gatagatata cataaaaata tatatgataa
    67921 taagttattt gaggagctaa agattgattg taagaagtgt tttggacttt gttgtgtagc
    67981 tctttatttt tctgcttcag atggatttcc tatcgataag gaatcaggaa aaccctgtat
    68041 taatttacaa ccagatttta aatgttctgt acataatagt cttatgaagc gaggttttaa
    68101 agggtgtact gcatatgatt gttttggttc aggtcaaaaa gtagcacaag taacatataa
    68161 ggggatagac tggatgcaat caccagaatt aacaaatcaa atgtctgaag tgtttttaat
    68221 tatgagacaa ttacatgaaa tgttatggta tcttaaagaa gcatctgtat taaatatatc
    68281 agatacaatc aaatcaaaaa ttgatttgat aattgaagaa actgaaaaga taactaatat
    68341 gagcccagaa cagataatta atttggatat catatcacat agaacaaagg ttaatttatt
    68401 attatcacaa gcgagtgagt ctgtgatggg aaaggttaaa tcatttatca agacttctac
    68461 tcttaaaaat atgaaaaaat taagtaaaaa tatagattta ataggagcag accttagaaa
    68521 aataaatcta ataggagcag atttaagagg tagatttcta atagcagcaa atttaagaag
    68581 tacagatttg agtggggcaa atttaatagg agcagactta agagattgtg atattagagg
    68641 tgctaatctt gaaaatagta tatttttaac acaattgcaa gtaaatactg caaaaggaga
    68701 ttctagtacg aagttaccag cttcactaat acgccctaaa tactgggaga aataattaaa
    68761 ttaatacata attttacaaa atatgaactt ttttgacttt aattcatatt atacattaga
    68821 gattctttac aagaatttct taatataata tagggagaga tataaaaatg agttatcaaa
    68881 attataaatg tgaaagagat tatgtgtatg atgattataa atacaataag tgcaacaagt
    68941 gctataatga ttatgaggaa atgacacatg ttcatgaata ttcagaaagt gttaagttag
    69001 cagaagaatg tgaggataga cataaccacc gtgcagcagg ggttactggt gaagctatac
    69061 caataaatgg aggtacaaat catgttcata aaataaatga taatgtagat ttccttgacc
    69121 atttccataa gatatgtgtt acaacagggc cagcaattag aatacctgga actgataaac
    69181 acattcactt aatatgtgga gaaactactg ttaatgatgg tcattgtcat aaattccttt
    69241 ttactactca aatagaagca cctttagtat aaactgatat ggattattgt agtaaatttg
    69301 ctagattata gtatataata aatgttaaaa atatataata gataatattt aaacttaatt
    69361 aggactatga tacttaacct tcgtagtcct ttctaagttt tgtataaaaa agtgttatac
    69421 aattaaaagt gatataggga ggaaatttta tgaaatctgg aataattctt gaaatgaaac
    69481 aaataaattc aacagaatat gacgaaatgt tgcaagtctg ggaatcatca gtaagagcaa
    69541 cgcatgattt tttaacagaa aaagatattg aatctttgat accattagtt gaaattggac
    69601 ttaaagaagt agaaaatata gtttgtataa aagataatga tataataaag ggctttatag
    69661 gaatagataa agataaaatt gagatgctct ttatagaaga taaatataga ggaaatggta
    69721 ttggtaaaaa attaataaaa tatgctatag ataaatacaa tgtaaaatat gttgatgtaa
    69781 atgagcagaa taaaaaagct gtaggatttt atattcactt aggatttaaa gtgtttgata
    69841 ggtctgaaat agatggacaa ggtaacccat ttccaatact acatatgaaa ttaatttaaa
    69901 gttatacttc tcacttattc gcaaaaatat ttacttaaat ttttatacaa ataaaagtcg
    69961 tgacagtctt aggacacgac ttttttacta cactattaaa ttgaaattta tctgttctat
    70021 attatgatta ttagatgtag tttttttatt tttactatgt acttaaatac caagtatacc
    70081 catacctatc aagtacttca gcagcgccat cattttgatg tggtggtggg tgtggtaaaa
    70141 attcttgtac aagtgtacca ccctcggcaa gttttacaga tatttcttct atcgcttctc
    70201 gagatggtag aacaacacaa atcgccatgc cagaaattac aacaggcttg tctgtatccg
    70261 cacccattat acaaccttcg tattttgtga aagtaacact tgcgtgcata ataggattgt
    70321 ttggattgct gtctaatcgc gcaaatatag tttttcctcc tccgaaagcc tcagcataaa
    70381 aattaaatgc tttttcacaa gtaccattaa atattaaata tggagttagc atgaccaata
    70441 tatcctcctt gatatagaat aatttatagt tgcacctgat aatctaaatt atgatttagt
    70501 aaattgtatc atagggaaac cttgaagtat ataatttttt attattatat tatacaaatt
    70561 tattaattta aaaaaatgtt atatgtacgg aaggtttaca ggataaaata taataaaaag
    70621 attaaggtga cattataaaa aatgtatatt gacattgacg ctacgtaatt gtatatactt
    70681 tgtcttaaag gagatggtaa atatgaatat tatagaaatt cgctcagata aaatatacaa
    70741 gaagataatg gatgcaccaa taaacaaaaa agaagatata tacagatatg aattgatgaa
    70801 gccttttgaa tttaagtgga agtgtatgaa tgttccaata gttgctagac agaaaggtgg
    70861 atatgatgta attatagcaa gtgaaatgtt aggggtttta tcgcctaagg atattgatga
    70921 aaagcaaaaa aagaatataa atgtgttatc tgctgataaa atttggggca cttgcaaaga
    70981 aaccatagaa aactctataa atgcttttat aaaagaaggg tatgatttaa acattaagga
    71041 ctataaatat tcaatattat tggcgaatcc aaatagtcct tatacaatat taagtgatgg
    71101 atactggggt gatggtggga ttcctggata tatatttcta tcattggttc ctaatgaata
    71161 tactatcaat agattaccag tattaatagc acatgaatgt aatcacaata ttagatttca
    71221 gtttatagag tggaataata atataacatt agaagaaatg atgataaatg aaggtcttgc
    71281 agaaaatttt gcaacatgga tgtttggaga ggaaatgtta ggaccttggg tcagtagaac
    71341 agatatcgaa acattaaata cttatataaa gccaataata aaaagtgctt taaaagaaac
    71401 tggatttcaa aatataacat cttatcttta tggtgatgat atagctaaaa tgcaaggata
    71461 ttttccagta gggttgcctt attgtgcagg atatgcttgt ggatattata tgattaagta
    71521 ttatttagaa aagacaaata aatcaataat cgaagcgact ttattgcctt atagtgagat
    71581 aatcgaaaca gtaaaagagt tttgggaata aattttataa attgcataat ttgaaataaa
    71641 ttatgcaatt attttatttt taagaaaaaa taaattgaca ataatttata agaaatatac
    71701 aataatatat ataaagaaat atttcaatat ttaaaagttt taagatttca tatctagagt
    71761 acctaacatt acatgttagt ttcttgttac ttatctaatt actttatatt tcgtagttat
    71821 atataaaatt actataaaaa atgaaatata ttaaacaatc aactaaaaaa tgaaatatta
    71881 ataaaaaaaa ttcaaattat aaatattaaa tgaatataat taaaacaaat atataaattt
    71941 aagtataaca ctcacatttt aatgtattgt gaaatttttg ttgaacttta tgtaccataa
    72001 cttataaaat ttatgtcatg ttatatttga ataagtaata aaattgtaga tgcaaattaa
    72061 tctacaaaat ttatatgaaa tggagttgat gctatgacag aattaatgat tataagtggg
    72121 ttagtactat tgatttgtat aacttctagc aaggtattgt ataaatttgg agtaccaata
    72181 ttattgattt ttatcttact agggatgtta tttgggtctg atggaatggt aggaatcta
//
LOCUS       DAMKPG010000007        63037 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMKPG010000007 DAMKPG010000000
VERSION     DAMKPG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63037
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.008"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            201..1076
                     /locus_tag="PC621_000540"
     CDS             201..1076
                     /locus_tag="PC621_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HDF4107726.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1094..2584
                     /gene="cls"
                     /locus_tag="PC621_000541"
     CDS             1094..2584
                     /gene="cls"
                     /locus_tag="PC621_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF4107727.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2777..3061
                     /locus_tag="PC621_000542"
     CDS             2777..3061
                     /locus_tag="PC621_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF4107728.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3094..4722
                     /gene="groL"
                     /locus_tag="PC621_000543"
     CDS             3094..4722
                     /gene="groL"
                     /locus_tag="PC621_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF4107729.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            4984..5724
                     /gene="larB"
                     /locus_tag="PC621_000544"
     CDS             4984..5724
                     /gene="larB"
                     /locus_tag="PC621_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF4107730.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5808..6542
                     /gene="larC"
                     /locus_tag="PC621_000545"
     CDS             5808..6542
                     /gene="larC"
                     /locus_tag="PC621_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF4107731.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6572..6991
                     /locus_tag="PC621_000546"
     CDS             6572..6991
                     /locus_tag="PC621_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF4107732.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7051..7152
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7373..8908
                     /gene="guaA"
                     /locus_tag="PC621_000547"
     CDS             7373..8908
                     /gene="guaA"
                     /locus_tag="PC621_000547"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF4107733.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9447..10877
                     /locus_tag="PC621_000548"
     CDS             9447..10877
                     /locus_tag="PC621_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4107734.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(11025..11900)
                     /locus_tag="PC621_000549"
     CDS             complement(11025..11900)
                     /locus_tag="PC621_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4107735.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            12020..12583
                     /locus_tag="PC621_000550"
     CDS             12020..12583
                     /locus_tag="PC621_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF4107736.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            12580..13146
                     /locus_tag="PC621_000551"
     CDS             12580..13146
                     /locus_tag="PC621_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF4107737.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(13275..13511)
                     /locus_tag="PC621_000552"
     CDS             complement(13275..13511)
                     /locus_tag="PC621_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107738.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13821..17249)
                     /gene="uvrA"
                     /locus_tag="PC621_000553"
     CDS             complement(13821..17249)
                     /gene="uvrA"
                     /locus_tag="PC621_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF4107739.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            17568..19802
                     /locus_tag="PC621_000554"
     CDS             17568..19802
                     /locus_tag="PC621_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4107740.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNK
                     LNSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            20058..22088
                     /locus_tag="PC621_000555"
     CDS             20058..22088
                     /locus_tag="PC621_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4107741.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            22085..22537
                     /locus_tag="PC621_000556"
     CDS             22085..22537
                     /locus_tag="PC621_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4107742.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            22539..23594
                     /locus_tag="PC621_000557"
     CDS             22539..23594
                     /locus_tag="PC621_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF4107743.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            23661..23969
                     /locus_tag="PC621_000558"
     CDS             23661..23969
                     /locus_tag="PC621_000558"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF4107744.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            24073..25338
                     /locus_tag="PC621_000559"
     CDS             24073..25338
                     /locus_tag="PC621_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF4107745.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            25426..26142
                     /locus_tag="PC621_000560"
     CDS             25426..26142
                     /locus_tag="PC621_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF4107746.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(26337..26630)
                     /locus_tag="PC621_000561"
     CDS             complement(26337..26630)
                     /locus_tag="PC621_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF4107747.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(26636..26851)
                     /locus_tag="PC621_000562"
     CDS             complement(26636..26851)
                     /locus_tag="PC621_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107748.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            complement(27396..27908)
                     /locus_tag="PC621_000563"
     CDS             complement(27396..27908)
                     /locus_tag="PC621_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4107749.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            28358..28834
                     /gene="purE"
                     /locus_tag="PC621_000564"
     CDS             28358..28834
                     /gene="purE"
                     /locus_tag="PC621_000564"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF4107750.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            28840..29538
                     /locus_tag="PC621_000565"
     CDS             28840..29538
                     /locus_tag="PC621_000565"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF4107751.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            29550..30917
                     /gene="purF"
                     /locus_tag="PC621_000566"
     CDS             29550..30917
                     /gene="purF"
                     /locus_tag="PC621_000566"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF4107752.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            30911..31987
                     /locus_tag="PC621_000567"
     CDS             30911..31987
                     /locus_tag="PC621_000567"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF4107753.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            31981..32574
                     /locus_tag="PC621_000568"
     CDS             31981..32574
                     /locus_tag="PC621_000568"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF4107754.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            32567..34099
                     /gene="purH"
                     /locus_tag="PC621_000569"
     CDS             32567..34099
                     /gene="purH"
                     /locus_tag="PC621_000569"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF4107755.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            34110..35360
                     /gene="purD"
                     /locus_tag="PC621_000570"
     CDS             34110..35360
                     /gene="purD"
                     /locus_tag="PC621_000570"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF4107756.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKVYCAPGNAGITNVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMETILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMLDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            35385..39191
                     /locus_tag="PC621_000571"
     CDS             35385..39191
                     /locus_tag="PC621_000571"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF4107757.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAVPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKTFTDDKLFE
                     ASYGNIVLELANDDLCMLEGYNYVVLGSTTKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERVGA
                     NANIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVFKNIIGEKDQKIFES
                     GVKYFR"
     gene            39397..40269
                     /gene="rfbD"
                     /locus_tag="PC621_000572"
     CDS             39397..40269
                     /gene="rfbD"
                     /locus_tag="PC621_000572"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF4107758.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            40378..41259
                     /gene="rfbA"
                     /locus_tag="PC621_000573"
     CDS             40378..41259
                     /gene="rfbA"
                     /locus_tag="PC621_000573"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF4107759.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            41296..41853
                     /gene="rfbC"
                     /locus_tag="PC621_000574"
     CDS             41296..41853
                     /gene="rfbC"
                     /locus_tag="PC621_000574"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF4107760.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            41891..42874
                     /gene="rfbB"
                     /locus_tag="PC621_000575"
     CDS             41891..42874
                     /gene="rfbB"
                     /locus_tag="PC621_000575"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF4107761.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            42913..43581
                     /locus_tag="PC621_000576"
     CDS             42913..43581
                     /locus_tag="PC621_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF4107762.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            43590..44018
                     /locus_tag="PC621_000577"
     CDS             43590..44018
                     /locus_tag="PC621_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF4107763.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            44042..44356
                     /locus_tag="PC621_000578"
     CDS             44042..44356
                     /locus_tag="PC621_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF4107764.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            44508..44783
                     /gene="flgM"
                     /locus_tag="PC621_000579"
     CDS             44508..44783
                     /gene="flgM"
                     /locus_tag="PC621_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF4107765.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            44788..45204
                     /locus_tag="PC621_000580"
     CDS             44788..45204
                     /locus_tag="PC621_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF4107766.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            45222..46532
                     /gene="flgK"
                     /locus_tag="PC621_000581"
     CDS             45222..46532
                     /gene="flgK"
                     /locus_tag="PC621_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF4107767.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            46551..47483
                     /gene="flgL"
                     /locus_tag="PC621_000582"
     CDS             46551..47483
                     /gene="flgL"
                     /locus_tag="PC621_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF4107768.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            47499..47891
                     /locus_tag="PC621_000583"
     CDS             47499..47891
                     /locus_tag="PC621_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF4107769.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            47885..48097
                     /gene="csrA"
                     /locus_tag="PC621_000584"
     CDS             47885..48097
                     /gene="csrA"
                     /locus_tag="PC621_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF4107770.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            48119..48514
                     /gene="fliS"
                     /locus_tag="PC621_000585"
     CDS             48119..48514
                     /gene="fliS"
                     /locus_tag="PC621_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF4107771.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            48514..48870
                     /gene="fliS"
                     /locus_tag="PC621_000586"
     CDS             48514..48870
                     /gene="fliS"
                     /locus_tag="PC621_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF4107772.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            48891..50414
                     /gene="fliD"
                     /locus_tag="PC621_000587"
     CDS             48891..50414
                     /gene="fliD"
                     /locus_tag="PC621_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF4107773.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            50417..50752
                     /gene="fliT"
                     /locus_tag="PC621_000588"
     CDS             50417..50752
                     /gene="fliT"
                     /locus_tag="PC621_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF4107774.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            50854..51726
                     /locus_tag="PC621_000589"
     CDS             50854..51726
                     /locus_tag="PC621_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF4107775.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            51819..53960
                     /locus_tag="PC621_000590"
     CDS             51819..53960
                     /locus_tag="PC621_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF4107776.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKSLVDDYSKTFMDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            53984..55861
                     /locus_tag="PC621_000591"
     CDS             53984..55861
                     /locus_tag="PC621_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF4107777.1"
                     /translation="MNTPIVVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFMFAHKINTSNINLWKYRLKLVGFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFR
                     MLESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYEKDFFDYIILGDVLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSVTATSMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKRYSSISMVNLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLVDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRY
                     E"
     gene            55854..57968
                     /locus_tag="PC621_000592"
     CDS             55854..57968
                     /locus_tag="PC621_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF4107778.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            57977..58972
                     /locus_tag="PC621_000593"
     CDS             57977..58972
                     /locus_tag="PC621_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF4107779.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            59064..60296
                     /locus_tag="PC621_000594"
     CDS             59064..60296
                     /locus_tag="PC621_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF4107780.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            60316..61260
                     /locus_tag="PC621_000595"
     CDS             60316..61260
                     /locus_tag="PC621_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF4107781.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            61354..61761
                     /locus_tag="PC621_000596"
     CDS             61354..61761
                     /locus_tag="PC621_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HDF4107782.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            61848..62939
                     /locus_tag="PC621_000597"
     CDS             61848..62939
                     /locus_tag="PC621_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF4107783.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
BASE COUNT        24906 a         6640 c        11285 g        20206 t
ORIGIN      
        1 ttaagtgggt ataaacttat agaaattcat tttttattta aggtaaaaat ttaaagtaaa
       61 atattatttt acataataat aaccttataa attttgaaat tacttatctt tgtgttaaaa
      121 taagtaaaat acacatatta aagttaagtt tttaggcaaa taaacaaaat gaaaatataa
      181 atattaagga gatttggtat atgaatgttt atgaaaaagg taatggagtt atactagaag
      241 gagtaacaga ttttgaccca gtacatatat ttgagtgtgg tcaatgtttt agatggcaca
      301 aacaagaaga tggctcatac actggagttg caagggggag aatattaaat gtcaaaaaag
      361 agaatgataa aatatattta aataatacga atttaaagga gtttaacagc atttggtata
      421 attactttga tttaggaact gattatacag aaataaagaa taagctaaaa aatatggatg
      481 aatatttgaa taaggctact gaatttggat ggggaataag aatattaaga caagatggat
      541 gggaaatgct tatatcattt ataatttcct ctaataatag aattcctatg atacaaagag
      601 cgatagaaaa tttatcaaga aagtttggta agtatatagg tgaatatgaa ggtaatgaat
      661 actatgcatt cccaacacca gaagaactaa ataaggcctc acaagaggaa ataagagcat
      721 gtcaaacagg ttttagagat aagtacataa aaagtactac acaggcagtt atagagaata
      781 atgatgaggt atctgaatat acaaatttaa gtacagagga ttgtagaaaa gagcttttaa
      841 aatttaatgg agtaggacca aaagtatgtg attgtattgc attatttggt atgcaaaaat
      901 atgattcttt tccagttgat gtttgggtta agagagttat gcaagaattt tatatagatg
      961 aagacatgag tttacctaaa atgagaactt atggaatcga taaatttaaa gaaatgtctg
     1021 gatttgctca acagtactta ttttactatg caagagaact cggtatagga aaataaatta
     1081 agggggagaa attatgggag taataggaac aatatttctg ttctatttaa ttatatcata
     1141 tctagctgga gccataattt ctgtgattat attgcttgaa aatagagacc ctgcaaaaac
     1201 aatgtcttgg ctattgatgt ttattatatt tccaggtgta ggtttaatga tatatgctat
     1261 ttcaggtaga aatattagaa aaagaaaatt atttaagact caaaaactag ctaataatat
     1321 aaaagaaaag aaactatttg atacattaga aaagataaca gaaatagttg aattagaaaa
     1381 agaatctata aaacaaaata agcttttaag agatgaagag gatggtagtt atagaaaaag
     1441 ggtaataaat atgttgttaa agactggtat gtttcccttt actaaaaata ataaagtgga
     1501 tgtatttgta gatggaaatg aaaagtttaa aagacttata gaagatataa gagaagctaa
     1561 agaccatatt catttagagt actttatcat caaagatagt gaaataggta gggtgttaaa
     1621 agaagaactt ataaaaaagg ctaaagaagg cattaagata agaattttat atgatgatgt
     1681 aggatgctgg agattttggt ttaatagaaa atttttccga gaaatgaggg aagtaggtat
     1741 agaaattgca gcatttttac ctacaaaatt tcctataata ggagggaaat taaactatag
     1801 aaatcacaga aaaattgttg ttattgatgg aataattgga tatacaggtg gaataaatat
     1861 aggagatgaa tatttaggca aaaatgataa gtttggatat tggagagata ctcatattag
     1921 gattaagggt atttctgtat acatgcttca aatgacattt ctgattgatt ggtattatac
     1981 gacaaaagaa gtattagtga ctaaaaatta tttcccaagt gtagggaatg ttggtgaaag
     2041 tatgatacaa gttgttgcga gtggtccaga cagtgactgg gaagatattc attatgctta
     2101 tttttctgca atatgtcagg ctagaaaaaa tgtttatata gaaactccat attttattcc
     2161 agatgaaagt ctactaaaag ctataaagag tgctgctctt agtggagttg atgtgagaat
     2221 aatatttcct aaaattgcag accacaagat agtaaatata gcatcttatt catattttga
     2281 agaaatatta agagcaggtg gtaaagttta tttatataat aaaggattta tacattctaa
     2341 agtcgtaata atagatgata aaattgcttc tgcaggtaca gctaatatgg atttaagaag
     2401 ttttatgctt aactttgagg taaatgcatt tatctatgat gaagaagtta ttagggtaat
     2461 gacagatgat ttctttgagg atttaagtca ctgtgaagaa cttaatttgg aggtatttaa
     2521 gaatagaaat atcatacaaa aaatcaaaga atctgtagca agattatttt caccaatatt
     2581 ataaaaaatt attaaaaaag gtattgaaaa ttatgtgtga ttggtatatt ataataattg
     2641 ttagcactct attagataga gtgataataa ataaaaagta tatatataaa tctacatata
     2701 taagtagtat taatttaaaa ataactaaaa acataggcga actagtctat acttaatatt
     2761 ttaggaggcg tgaataatga aaataagacc attagctgac agagtagtaa ttaaaaaagt
     2821 agaagcagaa gaaaaaactg caagtggaat agttttacca ggagcagcta aagagcaacc
     2881 tcaaatagct gaagttgtag aagttggacc aggtggaata gttgaaggga aagaaataaa
     2941 aatggaattg acagtgggag ataaggttat attccaaaaa tattctggaa cagaagttaa
     3001 gatagaagga caagaataca caatactaag acagagtgat gtattagctg ttattgaata
     3061 aatatagaat aaatttatta ggagggattt aaaatggcta aagaaattaa attttcagag
     3121 gaaacaagaa gagctttaga agctggtgta aataaattag cagatacagt aaaagtaaca
     3181 ttaggaccta aaggaagaaa tgttatatta gataaaaaat ttggttctcc acttataact
     3241 aatgatggag taactatagc aaaagagata gaactagaag atagatttga aaatatgggt
     3301 gctcaattag ttaaagaagt agcaactaaa actaatgatg tagctggaga tggtactaca
     3361 actgctacag ttttagcaca agctataata agagaaggtt taaagaatgt aacagcaggg
     3421 gctaacccaa tacttttaag aaaaggaata caaaaagcag taatagtagc ggtagaagaa
     3481 ttaaaaaatc aatcaagaat agtagaaaca caagaggcta tatctcaagt tgcttctata
     3541 tctgctggtg atgaagaagt tggaaaatta atagcagaag ctatggaaat agtaggtaaa
     3601 gatggagtta taactgttga agagtctcaa actatgaata ctgaattaga tgctgttgaa
     3661 ggtatgcagt ttgatagagg atttgtttct gcatatatgg ttacagatgt agataaaatg
     3721 gaagcagttt taaatgaccc atatatatta attactgata agaaaatatc taacatacaa
     3781 gaattattac cagttcttga acaaatagtt caacaaggta aaaagttatt gataatagct
     3841 gaagatgtag aaggtgaagc attatctaca ttagtagtaa ataaattaag aggaacattt
     3901 gatgtagtag cagttaaagc tccaggcttt ggagatagaa gaaaagaaat gcttcaagat
     3961 atagcaatac ttacaggtgc tcaagtaata tcagaagaat taggttatga tttaaaagaa
     4021 gctgatttat ctatgctagg tagagcttca tctgttaagg taactaaaga aagtactaca
     4081 atagtagatg gttctggaga taaaaaagct atagaagata gagtaactca aattaaacat
     4141 caagtagagc aaactacttc agattttgat agagagaaat taatggaaag attagctaaa
     4201 cttgctggag gagtagctgt tgtaaaagtt ggagctgcta cagaagtaga gttaaaagaa
     4261 agaaaattaa gaatagaaga tgctcttaat gcaactagag cagctgtaga agaaggaata
     4321 gtagctggtg gaggaactgc ttttgttagt gttataccag caataggaac actaattgag
     4381 agtttagaag gagaagttaa actaggtgct caaatagtta aaaaagcgtt agaagagcca
     4441 ttaagacaaa tagctataaa tgctggtctt gaaggtgctg taattgtgca aaatgttgtt
     4501 aattctgaag cagaaactgg atttgacgca ttgaatgaaa aatatgtaaa tatgatagaa
     4561 gctggtatag ttgacccaac taaagttagt agaagtgctt tacaaaatgc tgcatcaata
     4621 gcaagtactt tcttaactac tgaagctgct gttgctgacc ttcctgaaaa agaagatgca
     4681 ggaatgccag gaatgggtgg cggaatgcct ggtatgatgt aatttaaaaa atttaaaata
     4741 taaattttaa tataaagaac tatttcgact tagattttaa actctaagtt gaaatagttc
     4801 ttttgtatat atctaatttt tgtatatcta taaattcaat acacatattt agataatcag
     4861 tatctagttt gtaataatat tttagagtag tattgttatt ttttaggtta tgattttaga
     4921 tatatattaa ttaaggtata attgttgata gagaataaat caaatttaaa aggatgcgat
     4981 aatatggact taagaaaatt acttgaagaa gttaaaaatg aaaatattga tatagatttt
     5041 gcgttagaaa agttaaaaga tttaccttat gaagaccttg gatatgcaaa tatagaccat
     5101 catagagaat taagaaatgg gtatccggaa gttatttatt gtgaaggtaa gagtgatgag
     5161 catattttag ggattattga taaaatgaat caaaaaagtt ctaacatatt ggggacaagg
     5221 tgcagaaaag aaacttttct gaaaattaaa gaaatctata atcatgctga gtacgaagag
     5281 gcgtctaaaa ttcttaagat tcaaaatcat gatatagaga atataggaaa aggtaaaatt
     5341 gtgattgcta cagggggaac ttctgatata ccagtagcag acgaagctta tcatacagct
     5401 aagtttttag gtaatgatgt tgatagaata tatgatgttg gagtggcagg tatacatagg
     5461 cttttaaata aaagacataa aatagatagt gctagagtaa tagtagccgt tgcaggtatg
     5521 gaaggtgctc ttgcaagtgt aattggtgga ctggttgatg ttccagtaat agctgttcca
     5581 acttctgttg gatatggagc aaattttgga ggattagcag cattactagc tatgttaaat
     5641 agctgtgcat ctggaatatc tgttgtaaat attgataatg gatttggagc aggatattta
     5701 gcatcaatga taaataaact gtaaatacat tgatataaat taaacaaaac tatataataa
     5761 aatttaattt ctattatatt tttactatta aaattgagga gacatatatg agtgaaaaaa
     5821 tactttattt tgatataata agcggtatat caggggatat gacccttgca agtttattaa
     5881 atttaggtgt gcctaaggaa atttttttgg aggaaattca taaactaaga atgagcgatg
     5941 aatttaatat aaacatcagc tcgaaaactg aaaatggaat agtaggtaca aaagtagaag
     6001 taattacaaa agaaaaacat acacatagaa atttagtgga tatatttgag gttatagata
     6061 aaagcactct aaacgaaaat gtaaaatcta aagcaaaaaa aatatttatg gtaattggag
     6121 aagcagaagc aaaagttcat ggaactacaa tagacaagat acattttcat gaggtagggg
     6181 ctatagattc aattgtagat atagtaggag cttctatatt agttgactta ctttgtgtag
     6241 ataaagtttg tgctagtaca gtgtctgttg gttcaggatt tgtaaaatgt gaacatggaa
     6301 ttatacctat acctgcacct gcaactatag aaattttaaa aggtgtgcca attaaattaa
     6361 ataatgtaaa tggagaatgt acaactccaa caggagctgc aatcataaaa gtcttgtgtg
     6421 atgagtttgt agatgagctt gaattttctc caaagcaaat tggatatggt ataggtcata
     6481 agaagtttga agtaccaaat atactcagaa ctgtattagg tgaaaaaaaa aagaagaatt
     6541 aatttatgaa attagtgcaa acattgatga tatgtcttca gaaatctatt cttatttgta
     6601 tgaaaaaata ttagatgaga aagcattgga tatttataca gagagtattt atatgaagaa
     6661 gaatagacca gcaacaaaag tatcaatttt atgtaatgaa tcagatttag aaaagtttat
     6721 taaaatatta cttttagaaa ctagtacttt tggagtgaga tataatgcat ataatagaga
     6781 aattttagag agaaaatttg taaaattaga tacaaaatat ggagtggtta cagtaaaact
     6841 agggtattat aaaggaaaac tagtcaaggt tactccagaa tataatgaat gtaaaactat
     6901 agcaaaaaat gaagatattc ctataaaaaa agtgtttgct gatataaatt ttagaataaa
     6961 agaagaattt agtgaaaact tattgacata agtaataaat tttgataaat taatattcag
     7021 ccttattaaa taaaagtaaa cagaaaaaaa tatataaaat ataaactcac tcgtatatgc
     7081 tcagaaatat ggtctgagag tctctaccaa gataccgtaa atatcttgac tatgagtgaa
     7141 attattatac caaaagacct agttaagtaa atgtgtaggc tgagttatac tccctatgtg
     7201 ttggctataa gataaaggta ttttggaagc ctgtatagta atttcacttt tgctagaaat
     7261 tatggaagtt actatacagg ttttttgtgt aaaaagattt aatataattt agactgtatt
     7321 gtgttaaaat aaatatataa aaactatatt atacaaggag agatgtgaat ttatgaaaca
     7381 tgaattagta cttgtaatag attttggagg acaatataat cagttgatag ctagaagagt
     7441 tagagaaaac aacgtatatt gtgaaatact tccatgtact gcaagcatag aaagaataaa
     7501 agaaaaaaat cctaaaggga taatatttac aggcggtcca aatagtgcgt atttagaaga
     7561 ttctccaaca atatcaaaag aaatatttga actaggagta ccaatacttg gaatatgcta
     7621 tggtattcaa ataatgtctc atgtattggg tggagttgta agaaaaggta ataaacaaga
     7681 aaaggaatat ggaaaaacat ctataactta tggaaaatca tcattatttg aaggaataac
     7741 tacaaatagt gtatggatga gtcatacaga tttaatagaa aaagttccag aaggatttac
     7801 tatagttgca aatactaatg attgtccagt agcagctatg gaaaatgtag aacgaaacct
     7861 atatggagtt caattccatc cagaggtaga acactgtttg gaaggggaca agatacttac
     7921 aaacttctta tataatattt gtaaagtaaa aggtgactgg actacagatt cattcataga
     7981 agataaaata aaagaattaa aagaaaaaat aggcgataaa aaagctttat gtgctttaag
     8041 tggaggagtt gattcttctg ttgctgcagt tcttattcat aaagcaattg gggacaactt
     8101 aacttgtata tttgttgacc atggtttact tagaaaaaat gaaggaaatg atgtagaaag
     8161 aatatttaga gagaaatttg atataaattt aataagagtt aatgcagaag atagattctt
     8221 atctaaatta aaaggagttt ccgaaccaga agctaagaga aaaataatag gtgaagaatt
     8281 tataagagta tttgaagaag aatcaaataa attaggaaaa atggatttct tagtacaagg
     8341 aacaatctat ccagatgtta tagaaagtgg acatggaaat gctgctacta taaagtctca
     8401 tcataatgtt ggtggaatcc cagaagatgt agacttccaa gaaattgtag aaccattaag
     8461 agagttattt aaggatgaag ttagaaaaat aggattagag cttggtatag aagaaggatt
     8521 aatatttaga catcctttcc caggaccagg tctaggaata agagttatag gtgatgtaac
     8581 taaagaaaaa tgtgatatat taagagaagc tgatgctgtt tatatggacg agcttagaaa
     8641 agcaggttta tacagagaaa tatggcaagc ttttgcaaca ttaccagatg ttaagactgt
     8701 tggagtcatg ggtgatgaaa gaacatatgc ttatttagtt ggtttaagag cagttacttc
     8761 ttctgatgga atgacttctg actggtataa gatgccttat gatgtattag aaagaatttc
     8821 taatagaata ataaatgaag ttgatggagt aaatagagta gtttatgata taacttctaa
     8881 acctcctgga actattgaat gggaataaaa tatagaatgc cagaaatcca catgaataca
     8941 ggatttgagg tctctcttac ttctatatta tactggtttg atactatcaa tatttgccag
     9001 tatcttatgg attaataagc aaaaaacatc ctgctgaatg gtaaattcgg cggggtgttt
     9061 ttattttgtt ttgaaactcc tttgctctga aactcgaagt atttaacact gatgatgttg
     9121 ttatctttta gttccttttg tgtcatgctc tgaaagacaa attgatacaa taaaaaagta
     9181 atttcttaat cattgatgga gatatgtatt tatgttactc ttgatggtaa tttgagtaat
     9241 attgttcata gctttttata ttcacttaat tagaaaacca tttggtataa tcataaataa
     9301 acttgatgat ataatagaaa agatttataa ttattagaag tatataaaag tattttatgt
     9361 ctatgaaatg tatgtctata aaacatatag atgacttttg tatattaatg tatgaatatt
     9421 gatattatag aagaattgag ttgtcaatga tactaaatca aatatttata gaagatggag
     9481 ggattatgag aaataaaata attataggaa ttactttttc acttgtatta ctaatgtctg
     9541 gatgtgcttc aaaacaaaca gcagaaataa acgtattagc aactactgat ttacatggag
     9601 ttatacctta tgagttaact tcttatgtaa aagaagaaag aaaaaaagat aaaaatctaa
     9661 ctttagtaga tgcaggagat ttttttgata gtggagaagt gtttggttct agcatggata
     9721 gttattttag tgctagatat aaagatggtg ataataacac tgaaagttat attgagacgc
     9781 ctatttcaaa agatatgaaa gaggtaggtt atgatgttgc agttcttggg aatcatgaat
     9841 ttatatccaa caataaattt tatctagata atatgatatc tgattttgag aaacaaaaaa
     9901 taaatatttt atcggcaaat acgtataaaa aaaatggaga tagttataca aaaccataca
     9961 taataaaaaa tattaataca cctgaaggaa acgtaaaact aggaatttta gggcttacta
    10021 taaaagaagt tggtgagagg aaacagtgga aagatggaaa acttgttgac tctaagtctt
    10081 tagaactaaa agaccaagag ggctacaatg gtgaattgta tatgaatgat ttagtagaag
    10141 atgctaaaaa gtgggttggt ataatggaaa aagagaagcc tgacattata gttgctgtgg
    10201 cacatacagg tgagaaacct aagaaaccta gaaacccagg aaatagaata caagacttag
    10261 ctcaacaagt tgatggaatt gatgctatag ttgcaggtca taatcatgtt caaattgagc
    10321 aacatgatta taaaaataaa tcaggagaaa atgttatagt tactgagcca ggaaagcatg
    10381 gggaatatat ttctaagatt aattttaaat tagaaaaaaa caatgataaa tggaatatag
    10441 ttgacaaatc tagtaaatta attaaatttg aaaaaaatca agaaagtgat aaatttggag
    10501 aatttatttc ttcaattaat aaagtaagta aagataagaa agaaataaga ttatcagaag
    10561 taaagactcc tgaatgggat aaaatatatg tttttaaatc tggaacatct gcagatgata
    10621 tatataaaac aataggatat aaatggagaa atatatcatc tccaaagggt gatgacatgg
    10681 tgcagattgt tgtaatgaat aataataagc ctgttagcta tatatatggt gaagctgaag
    10741 ttatgccaat taatataaaa tgtgatgaat cagagtataa aaataatgtt gtaactattt
    10801 atcctaataa aaatgataaa tttaaaattg aaaaaggtaa gcaagaatat agtattaatt
    10861 taaaacatat acaatagaaa cttttatctt atttaagttt agattgtact taaaataaag
    10921 tgtgtattat tttaaaaagt gatgataatt aaaatcacaa cattaaggaa tagtacacac
    10981 tttttatgct aaaaaataga atgttaaaaa attaaacctt taaattaaag tacattgctt
    11041 atattatcca atattatttc caaaggttgt gtaatatttt tatccttatg taatattgct
    11101 aacattttat tttccatatc tatctcatct aattcaatca atcgcaattt tttatgtaag
    11161 agtgaatcta ataataaatt ttctggcaaa atggttatac ctaaccctgc ttctgcagct
    11221 ttaataagtg caaaagaatt aacactttcc caaacaggat aagctttttg gtttgaaaga
    11281 gataatacac tgtccaaagt atcgcgaata gcacttcctt gttcacgtaa caataacgga
    11341 aacgtgcata actgtcgtag agataaggtt tgttcagata aaggaaaagt tggagcacat
    11401 gcaacacata acttatagga acctaataaa attgtacgga atgccccctg tggttctata
    11461 ccttcccaaa aacatatatc agcttctcca ctttttaaaa tatccatagc tgaattagca
    11521 ctttcgacac gtacattcac tttaagagat ggaaatgaat tttttagtcg acttaaaatt
    11581 ggtggaagta aaaaagaggc aattgtaatg ctagaaacaa tgtttatagg tgtacattct
    11641 tctaaacgac tgattttttt gtctaaattg cgacaagcag ttaagatact acgtgcttca
    11701 tctaaaagag aaatgccaca gcgagtaaga catacccctc gtggtaatct gtcaaatagt
    11761 gatgtttcag cttgttgttc aagttcagct atagcatgag atacagcaga ttgtgtgata
    11821 tgtaattttt ttgcagcacc tgtaaaagtg cctgtttgtt caattgcttc taaaatctct
    11881 aaatatcgta tgtttatcat aataaatatc ctcactatac atgaataaaa ttaattaatt
    11941 ccattaaatt ctatcatttt acagattgca aagcaacagg tatactgaat aatatgaagt
    12001 gaaaagaagg aggaatattt tgaatcaaag cagaaaaata tatttttggc ttttaattat
    12061 taacttgttt attagcacat tcacttttgg cggagggtat gtagttgtac ctatgattag
    12121 acgttatttt gtaaatcaaa agcaattctt tacagaagat gatttgatga gtatggcagc
    12181 agttgcacaa tcaactcctg gagctattgc aattaattta tctgctttgg caggttatcg
    12241 agtagctggt actttagggg ttgtcattag ttgtacagca gctataatac ctccactcgt
    12301 tattcttgga ttagtatcag cattttacac cacttttata tctaatgcca ttgtaactgc
    12361 agtattaaaa ggaatgcaag ctggtgttgc tgcactaatt gtggatttga ttattgacat
    12421 gtgctctatg atattaaaac aacgttcttt gtttttatca actatgatac ctattgcttt
    12481 ctttgcaaac tttgtgatga gtataaatgt aatttttata ttatctgttt gctgttttgt
    12541 atgtgtaatg caagttttct ataagaagag ggaaaataaa tgaatacaat ttggaactta
    12601 ttttttatat ttttaaaaat aggcttgttt agtattggtg gtggttatgc aatcatacct
    12661 ttaatccaag agaaagtagt aaatcaaact ggatggataa gtgagaaaat gtttacagat
    12721 attataacta tttcacaaat gacaccaggg ccactggctg ttaatacttc tacttttgtt
    12781 ggacttcaaa ttggagggtt ttggggagca gttgcagcca ctttaggttg tgtattatgt
    12841 ggtgtagtaa tatctctatt tttatataat ttttttcaaa agcatcagaa ctctacttat
    12901 atatttgaaa tattaaatgg tctaaaatca gcttcattag gtttaattat ctcagctgcg
    12961 gctacaatta taatccttgc attatttggg acgaataaag tgtacttcaa ttctatattt
    13021 gagatgctaa actggactgc actaataatc ttttttgtta tactactgat agtacgtaag
    13081 tggaaaatta atcctattgt gattataata ataagtggta tactaggaat atttttttat
    13141 ttataagttt ttggtaagta aaggatatct atttttttat atcttaaatc caaatagcca
    13201 ataaagtaag gctatttgga tttttaataa attctgaaaa gataatcaga aacccatata
    13261 acatttaaat tatatcactg gttttctaca aaccaatcat aagcttcttg gcaaaatttc
    13321 tcactatcta tatagtaact attatgaaca ttattaattt caacaagttt ctttttacat
    13381 tttagtgaat tataatagct atcaaaatat tgggttttaa aatatctatc atgaagtgtt
    13441 ttaaagacaa gagtaggtat gttgactcca tcttttatct tactatcttc tgcaataaag
    13501 cattctaaca ttgtattaat tatctttgta ctatttttcc aaatatgagg atttttcttt
    13561 tcaaatattt tataaaattc attatcatag acttcattat aacctttata tttaggtggt
    13621 tttactgtta tgtgaaaatt aggaagaaac ttaaagaaaa aatataataa tttttatgtt
    13681 ctgttttata tattattcat attttatcac agataatata ctaaagttaa tttttttatt
    13741 gtacaactat tataataatt cttctataat ataagacatt ctataatttt atatttaagt
    13801 tcatgtgtat ttaattgttt ttatataaaa tgtccagtaa tactatttgg atttattttt
    13861 atttcatcag gagttccaga agcaacaatt tcccctccat ttattccact gtctggacct
    13921 atatcgataa tccaatcaga agcacgtata acctctgtat tgtgttcaat aattaaaagt
    13981 gaatgctgtt catttacaag ttttctaaaa gtaatcaaca aattttctat atctttaaaa
    14041 tgaagtccag tagtaggctc atccaatatg tacagcattc gttttttagt ttttgtccct
    14101 aattcttttg ctaattttat cctttgagac tctcctccgc ttaatcctaa tgtattttgt
    14161 ccaagcttta gatatccaag tcccacttca tctaataagg agagttttct atatatatca
    14221 gagcaatcac taaaaaatgt ttttgcttct gctacttcca tgtcaagtat atctgcaatt
    14281 gtttttccct tgtattgtat agacagtatt tctttttgat atctcttacc ttgacattca
    14341 ttacaagtta cccattggtc tgccatatat tgtagaataa tttttgtttt acctaatcct
    14401 tgacagtttg ggcattgccc ctttttgcta ttaaagctaa aatagctttc atccaataat
    14461 ttttgttttg cttgtggagt ttcagtaaat agagaacgaa tttcatcaaa aatattgata
    14521 tatgtagcag gtgtagaacg gctagatttt ccaataggtg tttggtccat taagataaaa
    14581 tcgtcaatag catcaaatcc agtaagtgca gtataatttt tatttgggat agatttttgt
    14641 ttcaattttt cttctagagc aggaagtaaa ctgtgaaata caagagaact cttaccagaa
    14701 ccactaacac ctgtaataga gcacatacag tttaagggaa tttctacatc aatatttttt
    14761 aagttatttg catggcatcc ctttaagctt aaccatttac ttggtataat tttattttgt
    14821 gaacttatct tattgtatgt actcaaatat ttgcctgtta tagaatttgg attttttata
    14881 atttcttggg ttgtaccttc tgcaatgatg aagccccctt ttgtaccagc atttggtcct
    14941 acatcaataa tataatcagc agacagtatt gtatccttat catgttccac tactataaca
    15001 gtattaccct tgtctcttaa ttcttctatc attttgatga ttagattatg gtctcttgga
    15061 tgtaaaccaa tagatggttc gtctaaaata taggttaatc ctactaattc acttcctagc
    15121 tgacttgata atcgtactct ctgcagttct cctccagata aagttggagc tgttctatct
    15181 gttgatatat agctaagacc tacctttaat agataactaa ctcgaatttt taattctgtt
    15241 aagatatcat ttaccaaaga tagctcattt ttttgaagtt gatttggtaa ttcacacaac
    15301 caattccata actgttctat cgtcattttt gttagttcag ggaagcgata acctttgatt
    15361 gtagtgaatc tagcctcaat gcacagcaat tcgccaccac atgtattgca aggaattttg
    15421 ttcatatatt gatgcaaggt attattttca gaactagatt ctctaaaaag tctttgaatg
    15481 tggttgattg ctcctgaagc aggccttcta atctttgatt ctcgtccctt tgattcaaag
    15541 ctaaattcat atatcttatc gtctgtacca tataagattg catctataaa atctcttggc
    15601 aattcattcc aaggtatatc taggtctata tttttatctt ttgctattgc atataattca
    15661 cctaccatcc aattaccatt tggctttttc cccctcaatt ttccaaaata gtaagtagct
    15721 ccatctaata ttgaaatagt tgggtctttt acaattaatt gaggattaac ttgataggta
    15781 aaacccaatc cattacaatc gtgacaagct ccagtatggg agttagcatt gaaggttgaa
    15841 gtatttatct tagaaaaaat agtttggcaa tgtggacaat aattttttgt attattcttt
    15901 ttttctaatg gagttttgca atatggacag taactttttc cgattgatac aaataacatt
    15961 cttaagtaat cccaaatgct tgtaagagta ccaacagtag aacgaggatt ttggcttcct
    16021 tttttctgtt cgatagctat agtaggggtt aatccctgaa gtaaatcaaa ttccggtttt
    16081 tgaagctgtt ctgaaagttt tgctccatta cctagatttt caaaataacg tctttttcct
    16141 tcttcataaa tcgtatcgaa agctaaagaa gattttccac taccacttac acctgtaata
    16201 acagttattt gattttttgg aattgtaacc tcaacatcct ttaaattgtt ttgccttcct
    16261 ccttttagat agatagagct tttattatga caaagtgaat ttttaattga tttatttatt
    16321 gcttttgcaa atttgaattt cttttctagt tcaagaggat tgcctaattc ttttatttcc
    16381 gaaataccta atatttttaa ctgagaaaag attgatttca aataatcttc aggcaatttt
    16441 tctattaaca aattaaggtc ttctattttt atatttccgc ttgataatat ctctgccatt
    16501 tcatctaata aatttttttc tgctcttgtt aattgattgt attttttgga acttaattct
    16561 gtccataaat cccatggaag caattctaaa ccaatcatat tattaaagtc atgtagaaga
    16621 ttacccttta cagaatgcca acctttccaa tttttattat atccaaagtc atctggatga
    16681 gcatgtcctt gtcgacatct tatccaggct tctgaaccag ttaaaaaacc atctgttttt
    16741 ccgacatcat gagtgtcaaa attaatttga aaataatttt tttgtatgtc atctatttgt
    16801 gcatctattc ttatccatct ctttgttaga gtatcatagt attctacaag ccagtggtct
    16861 tcataagtaa gctcattttc aaaataatta gcaaaacctg ctctcattct agcctcaaaa
    16921 cctcgatatc ttaggagcga tacaagaagt aaagaataat ctctgcacat tccaacaagc
    16981 ctttgttttg ggtttcttgg aatagttagt gggttagaat ttatttcaaa tattcttttt
    17041 aacatttgag gaattgtacg aagcaattcc tcatcagatt gttgcttatt aaaggatata
    17101 ccatataatt ttccttggtc tccatgaatt actaggcttt gaaccacttt taataactca
    17161 attggagtct ttggtaaatc ttgatattta ttttcaaatt ttttaggatt tgtgatgatg
    17221 ctttgatttt tatagttatt tattttcatt tatatttcac ctctctaaat ggatgatata
    17281 ccctgacatg gtgtcatatg caaggacaat ttagattttt tggccaaaat ttaaaaataa
    17341 ataaaaaatg atacagtgat aaattgtaat acaaatatag tttcaaattg catataatat
    17401 aattatttaa taaaaccatt atctatggaa tgttaatgaa atgttaagat ggcattcttg
    17461 aatattaaga aatagaatac tataatatag tgtgaaggat tcttagtaaa aaaatttgta
    17521 agtgttaata tacattaaaa aataggttta taatttggag gaaaaaaatg aaattatcaa
    17581 ggtataagaa aaatagttta gttctgtcta taattttatg ttcacttatt atttcaatcc
    17641 ttgtaattac tgtttttaat accgtaaaac tacacaatat atctaataaa caatctaaaa
    17701 actatttaaa cgatgtatct acacagattg taatgaatgt tgattctaaa atcaaattta
    17761 ttttatccga cttaagaata atggcagatt ttattaagca atatgaaggt gatagtcgat
    17821 atgagtattt atctaaaaga aagactagtt ataagtatta tgatgttgga ataatagatt
    17881 tagaaggtaa agctgagttt ttaagtggaa aaaagtttga cttaaagaat actgcttcct
    17941 ataaaaaagg aattgaaggt aaagaatact cagaagtaat agagtcaatg ggttttgttc
    18001 tttatagtgt acctatattt aacgataatc atgaagtgtc tagtatttta ataggtgtat
    18061 ccaataaaga aagcatggat gagattctta atatagatag ctttaatgga gaaggaacta
    18121 ttgaaattat taatagtcag ggaaaaactc ttttcatagg aaaaaataat gagttaataa
    18181 aagaccttag ctataagtat aaggattctg cagatgaacc ttgggcacaa aagatgctta
    18241 atgattttaa aaatgataaa tcaggaaata ttacaataac ctcatcaaaa ggtgtacaat
    18301 gcttattaac ttatcatcca attacaagag gtttaaatga ttggcatttt cttctcatag
    18361 tacctgaaga tgttgtactt ggtgaactta ataagttaaa ttcttttaca ataataatga
    18421 catttataac tacatgtatt attggaatta taacattaat tttatatatt ataagaagaa
    18481 aatatgtaaa tcaaatagaa aatattgcat atttagatag tataacaaat ggtataaatt
    18541 caactaagtt tagtatgcta gtcaaacctt taatttcaga atcacctgat agtacttata
    18601 tgatgatagc aatgaatata aaagatttta agctaataaa tgattgtttc ggtagtgaaa
    18661 aaggaaatat gacactaaaa catctttaca atattttgta taaaaacatt gatgaaaatg
    18721 aagaatttgt gtgccgtcat gatgcagacc ttttctattt gcttattaag aatagaccag
    18781 tgttagaagc cctggagtta ttatataaaa tagaagctga cacaaattat tttaataaaa
    18841 atagggaaaa tccatacttt ttaagactat cagtaggtat ttacacaatt gaaaatcata
    18901 atgaagattt aattacaata caagaccatg caaatacagc aagaaaaagc tttaataaaa
    18961 ctcatcaaag tgatttttct ttttatagtg atatagaaag aaaacgatta atatctgaaa
    19021 aagaaatttc aaatttaatg gaaaaagccc ttgaaaataa agaatttttt atgtgcttac
    19081 aacctaaaat tgatataaaa agtggaaaaa ttagtggagc agagtctctt gtgcgttgga
    19141 aaaattcaga aaagggcata atttatccaa gtaattttat tcctcttttc gaaaaaagtg
    19201 gatttatatg taagttagat ttatatatac tagaagaaac atgcaaattg atttctaagt
    19261 ggataaaaga aggtaaagaa gtgttgagaa tttctgttaa tgtatcacgt caacatttga
    19321 atgataaatt gtttttagaa aaatataagc atatatgcaa taaatacaat gtaccaacat
    19381 gtctgataga tttagaactt accgaatcta ttttcttaga aaatccagaa gctatagata
    19441 taatagagga tattcattct aatggattta aatgttctat agacgatttt ggatttgggt
    19501 actcatcatt agggatatta aaggatttta aagttgatat aattaagtta gaccgttcat
    19561 tctttgttag caaaaataat atagaccgtg gtaaggtagt tattaagtct attattgaac
    19621 ttagcaaaag gctaggtatg aaagtaactg ctgaaggaat tgaagaatta gaacaagttg
    19681 agttcctaaa aaatgtagga tgtgattata ttcaaggtta tgttttttct aaaccattat
    19741 taatttctga ttttgaaaat tttgcatatg aaaataataa aattaaaaat atccattatt
    19801 aaatgtacaa gataaaaaat ttaggcaagt tatataatta aaaatataac ttgtcttttt
    19861 tatttatata tttaataaaa atatatttgc agattttgta aaaataattt tcaaatgttg
    19921 taagttgtgg ctaaagcttt attgtattat tctgaatata gaaagatagt tactgtctta
    19981 gataaaaact gaaacataca taataatata aaatttgcta tattaataac tctaattatt
    20041 ttggaaggag ggaaaatatg ataagtggga gaatgttact catcatgaat tttctaaaag
    20101 gaaaaaataa gacaagttac aaggatatta gcaaacaact tagtatagaa gaaagaaaag
    20161 ttagatatga tataaataat ttaaatattg ttttgcgttc attaaacaag tcagtaatac
    20221 aaaagatgaa caaaggtgtg ttaattattc ctgatgactt ccaagtagta tgtataaata
    20281 ataacgaata tgtattttcc ttaactgagc gagtatctat tatgaaaatt atatcaatgt
    20341 ttaaaataga taatctaaac ttagaaaaat tgagtaaaga atttcaggtt tctagaagtt
    20401 caattaaaaa tgaccttaat gttttgtcaa gtgaattaga aagaaaccaa ataactatta
    20461 cttatgataa atcttttaaa ataagtggag aagaagacct tatatataag caaagattga
    20521 acatattaaa atcgtattct tatctttttg gtaaggaaaa atctgattta agtgtatttg
    20581 agaagtattt agtgaatgta attgaaaaaa tattcaaagg gatttatttg attgatatat
    20641 ataattggtc tatgaattta ttattgcaaa tgggatggac tttaaatgat gaatctttta
    20701 catggtatgt gtcaaacatt ttccttttta catggtatat acattcagat gaaaaacatc
    20761 ctttagaatc tgcttcatta agtgagccat gttttgaaaa taagtttgac caagaatttg
    20821 aaacaattat tcaaaaaaag ttgactaagg aacaaatttt tctgttaaag agttttgtat
    20881 tttttacaaa taaatatgca agtttaaacg aggatatgga tttgattagt acagaaatta
    20941 tagttcaaga cctaatttac aatatgtcag aaatattaga gattagcttt aaagaagata
    21001 tgattcttta taaaggatta ctcaatcata ttgccccatt gatagagaga atcaagcaga
    21061 atatacagat ttatgaggaa ttacttcatg taataccaga taaatatcaa tatgtacttg
    21121 aagctactaa ggtttctatt caaaaaaaca gagttttaag tcaaataaag aatgaaaatg
    21181 aaataatact tttggcaata catttcttag gaagtataca tagaaatgag aggaataact
    21241 acaaaaatat attgttagtt tgtggatttg gatatggtgt aattgctatg ttaaaggata
    21301 gtttagtaaa tgattaccag ataaatatag tagagagtat tccatcatat aagttacaag
    21361 attataaaga atgggaaagt attgacttca ttataacaac atcaaagata gtaatagact
    21421 tgcctaaaac aattattgag ataaatccat ttttacaaga agaggattat ttgaagtttg
    21481 aagaaaaagg gataaaaaga aagaacgtct taactaatta tatgtcaata aaaaaaaggt
    21541 tggacttttt agaagaagat gctcaaaaaa gagtattaag tattatatgt caagaacttg
    21601 gatacagtga tgagagaata ttatttagac aacttaattt aagtgattta atagggattg
    21661 ataccataag gataattgat gaagatatga aatgggaaga tgctgtaaaa attagttcaa
    21721 acatacttgc taattgtgat tttgtatcag cacaatatgc tgagaatatt atagatattt
    21781 taaaaaatgt gggtttttat gctgtaaaag ataacgaatt tgctttacta catggcaatg
    21841 atagttcatt agtcaaagta agttcgataa gtttattaat atgtaaagaa gcagttagtt
    21901 ttggagataa aaaggttaaa atcatatttt gtctagcaag cagagataaa aaagagcaaa
    21961 ttcctgcaat aattaatcta actaaaatgg tttataagac taattttatt gctatgttag
    22021 aaagtgcaaa gacaaaagaa gaagcagaag ttttaattca taaatatgag aaggaggtca
    22081 ctgtatgaat aaattagtta atgtaaaatg ttcatttaaa atacatgcta attcaaaaga
    22141 agaagctatt gtatcgttag ttgatgtagt atcaaaagaa ggatatctaa tagataaaaa
    22201 tcaatttttg gaagatgttt taaagagaga agaaacattg tcaacttata ttggacatgg
    22261 tataggtctt cctcatagtc aaagtgtagg tgttaaaaac tcttgtatta caataggaaa
    22321 gcttgatatt cctattgaat ggacagaaga aggagaaaaa gtggatttaa tatttttaat
    22381 atcagtaaca aaagacaatg aaaataatct tcatttaaag attttatcaa aattagcacg
    22441 tttattgatg catgaaagtt ttagaaatca aattagatgt tcagatgaac aaactgttta
    22501 taatcttata aaagaaaaaa tagaggagga agattaaaat gagaatttta aagtttttgc
    22561 aaaaacatct tatgacagca acatcttata tgattccatt tgtggttgca ggaggaatat
    22621 tatttgcttt atcagtaact ctaagtggac aagctgctgt tcctgaaaca ggctggttgg
    22681 ctaaactgaa tcaaattgga gctgctggct tggctttatt cattcctata ttaggtggat
    22741 atatagcatt tagtatggca gataaaccag gattagctcc aggtatgata ggtgcatatt
    22801 tagcaaaaga agtaaatgca ggttttatag gtggtatcat agcaggattt atagcaggat
    22861 ttgttgtttt acaattaaag aaaattaaat tagctcctac aatgagaact ttaggttcta
    22921 tatttattta tcctttgatt ggtacattga ttacaggtgg tataattgta tttttaattg
    22981 gagaacctat agcttctttt atgacatgga tgacaaactg gttaaatgga atgtctggtg
    23041 tttctaaaat tcctttaggt ggaatcttag gaggaatgat agcctcagat atgggtggtc
    23101 ctataaataa ggttgctgct acttttgccc aaactcaagt tgacacactt ccatatttaa
    23161 tgggtggagt tggcgttgca atttgtatac ctccaattgg tttaggatta gctacattat
    23221 tgtttccaaa aaaatttagc aaggaagaaa gagattcagg taaagcttcg ctattaatgg
    23281 gatgtgttgg aattacagaa ggagctattc catttgcaac atcagatcca gttagagtaa
    23341 taccttgtat tatggctggg tcgattatag gaaatattat ggctttttta ttaggatgtt
    23401 taaatcatgc accctgggga ggtctgatag tattacctgt agttgacaat aggttggggt
    23461 atattgcatc agtattaact ggagcagtag ttgttgcagt attaatgaaa ttagtaaaaa
    23521 aagacgtgaa agaagatgaa gaaattgaag aggatttaga tgaatcaatt gaattaatat
    23581 ttgaagaatt ataaatataa atggaaggaa tttgtataaa aaagtaaata taaaactata
    23641 aacttaaagg agataagaat atgaaaattg tagcagtaac atcttgtcct agtggagtag
    23701 cacatactta catgtcggct gaatcacttg aattatcggc caaaaaattt ggtatagaaa
    23761 taaaggttga aactcaagga agctctggaa tagataatga attgtcactt aaagatattg
    23821 atgaagcaac ttgtgtaata ttgactaatg atgttgagat tagaaatatg gaacgtttta
    23881 agggaaagaa ggtagtaaga atgagtgtat ctgagataat taagaaatct gatgcattaa
    23941 ttaagaaaat aaaagatact tttcaataaa aattgattta ttttacatat aactcaaaga
    24001 tgtttttaaa gaaattttta ttaaaagcat ctttgaaaat atcttaaaaa tagaataaaa
    24061 ggagatatag tcatgaaaaa attaccaata aagactgtcg tacaaagatt actagactta
    24121 caagaagagg ggaaaagtgc aactctttta ggaattggtc ctatgtcacc aaacttgtta
    24181 caagcaagtt ttgaactagc aaaagatgat gattttcctt taatgtttat cgcaagtaga
    24241 aatcaagtag atgcagatga actaggtggc ggttatgtaa atggttggaa tcaggaaact
    24301 tttacaaaag atattaagaa gattgccgac aaagttggat ttgatggttt atattattta
    24361 tgtagagacc atggtgggcc atggcaaaga gataaagaga gaaatgacca tttgtctgtt
    24421 gatgaagcaa tggagttagg aaaaaaatct tatttagcgg acattgaagc aggttttgac
    24481 ctattgatga ttgaccctac aaaagaccca tttgaaattg gaaaagtaat tccactagat
    24541 gtagtactag aaagaactgt agaattaatt gagtattgtg aaaacgagag aaaaagactt
    24601 aatctaccag acataggtta tgaggttggt acagaggaaa ccaatggagg tcttacttca
    24661 actgagacgt atgaaacatt tataactaga ctaaaggttg aacttgataa taggggtttg
    24721 ccaatgccaa catttatagt tggtcaaact ggtactttaa ctagaaagac agaacaagta
    24781 ggaactttta attttagaaa tgcttatgat ttagctcaaa tggctaagaa atatggagtt
    24841 ggtttaaaag agcataatgg cgattactta gatgatgtaa ctttattaga acatatacca
    24901 tctcaaatta ttgctacaaa tgtagctcct caatatggta ctgaagaaac tagggcatat
    24961 ttaaaattag cagaagttga gtgtaaatta gaaaaagagg gcctagttga taaaacttct
    25021 aatatcagac atgttcttct tgttaatgca attgaatgtg gacgttggag aaaatgggta
    25081 gtaggagaac aaaaaaatct aactactgaa gagatattta agaatgaagt tttatctaat
    25141 gaaatactag atattgcagg acattataca tttaataatg atgatgttaa aaaagaaata
    25201 gaagtgcttt atgataattt aagtaagaat aatattgatg gtcaaagatt tgttgtagac
    25261 catattaaac gtcctttgcg tgattatgca gaatgctata acttaaaagg tgttactacg
    25321 aggattttaa ataaataata ggacttatag tggtgagtat tgttatacta ggtatttata
    25381 gttataatta tacttaccac tattaaattt aggaggacta atattttgat taatttaatt
    25441 tttgatgtag atgatacttt atataaccaa ttaacgcctt tttacactgc atataataaa
    25501 gtgttttctt caataaagga tatttctatt gaagatttat atatgagtag tagaaagtat
    25561 agtgatgagg tatttcatat gactgaaaat ggagaaatgc caatcaaaga aatgcatatt
    25621 tataggataa tgaaagcttt tgaggagttg ggaaattcta ttacagaaaa agatgcacaa
    25681 agttttcaag atgagtatat atatcagcaa tcacaaatta cactaatacc agaagttgag
    25741 aggattttaa atttttctaa agaaagaaat attaatctag gaattataac taatggccca
    25801 tcaaaccatc aaagaatgaa gttaaagcag ttaaatattg aaaattgggt tgataaaagt
    25861 aatattttta tttctagtga ggttggattt tctaagccag atactaatat ttttagagta
    25921 gctgagaatg ttatgaattt agatagagaa aatacatatt atgtgggtga ttcttataga
    25981 aatgatgtat taggtgctaa aaaagctggt tggaagagta tatggttgaa ccatagagga
    26041 catgaggtag aagagttgtt ttataaacct gattttgtaa ttttggaaca taaggatttg
    26101 atatctttat ttataaaaat atgtagttat aaaaatatgt agtaaaaaaa tgttaataat
    26161 agaattgtat aaaataatta ataataacta tataaaatta tatgtttaga ttgtgagcaa
    26221 tctattttat gttaaaaata gattatttag aagaagggga aatcttaaaa aaatttgaat
    26281 atcccctttt tattatttaa ttaaaatgaa atagttagaa atatatttta tttagtttag
    26341 tcaataacat tttgggattc tttataacta catgttaact gttgaatagg cttatcatat
    26401 ggtttaaccc atcctttatt aatacttaat tctgtaagtg ctgtgtgacc ttctaccatt
    26461 tgagttagtg aagctgaata tatagctctt aattcaggtg tactacttgt taatgcagaa
    26521 ttaagataca tattggcaga tgctttagca gatgaaagca tacttaacat tattacttta
    26581 tcgtctatat aagtattgtt cttaattaga ttttctatca tatttctcat gttatctata
    26641 cctcctcaga aattagtatt tcattttcaa ctataaattg ttgtatagct ttaattcttc
    26701 cttctgttgc taatatacta gattctgatt gtctttttag gtcttcatct gatattaaca
    26761 tatttattgc tctttgcata agtaatccgt cagactccat ttttataact gctgctaaag
    26821 atattttttc agtttctgaa agtgttctca tttaatcatc tcctatattg taatttatgc
    26881 tttaatagta tgtctttctt ttcaaggaaa taaccattac acttttttac agtattttat
    26941 attctcttta tatcaaagtt tagtcaactt aatatattaa tataattttg taggatattt
    27001 atcattaaat atctataaaa taaattattt gaatattcat aaatcaaaat ttatttgtat
    27061 gtacttcttc aagagtcaat atgaattttt tcaaaaaatt aattttgatg ataatcatta
    27121 agggctatat ttttcctaaa gttgtgatta tcattattaa tttactaaaa tactttaatg
    27181 aaaaatagct aactttaatt gtaggatatt cttaatatat ttgaaataaa taaaaatatt
    27241 gaaggatata atattcaatt ggtatataaa taataaatga tagttaagat ttaccttaga
    27301 ggctaattag gtgttcagtc aaaattaatg cctttagaat acaaatgtat tctaaaggca
    27361 tttttactat gtattttcat ttatattact aaacattatc tatttttata tacatttcta
    27421 tgtattttat tttcatcaaa tcggtctata aattcatttt tttcttcact atatccaata
    27481 gatataagtg cataaggaac taattttact ggtaagtcta aaatttgttt aactttattt
    27541 actactttat ttttaggaaa tactcctgcc caacaagcag ctagaccttg attagtaact
    27601 tctaataata aattttgtgt gcaagcacct aaatcttgta tccatttacc aggttttaag
    27661 aatttatctc tatttccaag cactactata cataatggtg caaattctat atgctttgct
    27721 ctatgctgtg actttgagag ctctttcagc agttccttat catctactat aataaattcc
    27781 caaggttgag cattttttga tgaaggtgct tgcatagctg attttagtat ttcagttatt
    27841 ttatcttctt ctatattttt atctatatat gttcttattg aacgtctatt ttttataact
    27901 tctaacatac agattctcct ttaaataaaa ttatttctat ttatataata tcattacttt
    27961 aagttttagc tattacttat ataaagaata aactttgtgt tggttttatc taagtaaaag
    28021 taacattcag aactaaaaaa caagactata aatccaagat attaattaaa aaagaagtaa
    28081 taaaaaataa tatacaagat ttaaaaaaga gtattgagct taaaatttaa ttagaaaacc
    28141 aattttgaga taatctgttt tttattttaa caaaaaaaca taaataaagt tcgatacgta
    28201 gcgaatgaaa aaaatacaag aaatattttt tttaaaaaat ttcaaaaaag agttgataat
    28261 atatgtatat taatataaaa tttaaggtgt ataattcgta taataatacg aatgatttgt
    28321 agtgtaaaat aacttataaa cggagagggg aagtctaatg aaagtagcag tagttatggg
    28381 ttcaaaatca gattatccaa aattagaaga agggataaaa ctgcttgaaa aatatggaat
    28441 agaggttgta gcaagagcat tatcagctca tagaacacca gaacaacttt caatattcct
    28501 aaaagaaata gaagatgata ctgatgtaat aatagcagca gcaggaaaag cagcacactt
    28561 accaggtgta atagcttcac aaacattaat tcctgtaata ggattaccta ttaaatcatc
    28621 aactatggat ggactagact cacttttatc aatagttcaa atgccaaaag gaatcccagt
    28681 tgcaacagtt acaatagact taggtttaaa cgcagcttta ttggcattac aaataatgac
    28741 tttaaaatac ccaaaattaa aagaggattt gaaatcatat agagaagaga tggcacaaaa
    28801 ggtgttagaa gacgacaaga atttaagggg gtagcagaaa tgttattata tgaaggaaaa
    28861 gcaaaacaag tatattctac agataatgaa aatgaatatg ttgtttatta taaagatgat
    28921 gcaacagcat ttaatggaga aaaaaaagca gaaatatctt caaaaggtat actaaataat
    28981 aagatatcaa caataatctt tgaaatgtta aaagaaaata atataaatac tcactttata
    29041 aaaagtttat cagatagaga aatgttagtt aaaaaagtag agattttacc attagaagta
    29101 atagtaagaa atattgctgc tggttctatc tgtaaaagag ttggtcttga agaaggtgta
    29161 gttttcgatg agccaatttt tgaaataagt tacaaaaatg atgcttatgg tgacccaatg
    29221 ctaaatgatg attatgcagt ggcaatgaag ttagcaacta gagaagagtt aaaattctta
    29281 agagaagaaa ctttaaaaat aaacgagtta ctaaaagcat ttttcttaaa attaaaccta
    29341 aagttagttg actttaaaat agaatttggt aaggattctg aaggaaatat aatattggca
    29401 gatgaagtat caccagacac ttgtagactt tgggatgtaa atacaaatga aaagttagat
    29461 aaggacagat ttagaaaaga ccttggagac cttgtagaag gttatacgga agtactttca
    29521 agaatgaata ataaatagga ggcaaatgta tgtgtggagt tttagggatt tactctaata
    29581 aagatgtaac taaagaacta tattattctt tatattctat gcaacacagg gggcaagaaa
    29641 gctgtggttt agctctttta gatgatggag aaattaagta caaaaaagat atgggattag
    29701 ttggagatgt ttttaaggaa aatgaattat ctaagttaaa aggaaatata gggattggac
    29761 atgttagata ttcaacagct ggaggaagtc atgtttctaa ctgtcaacct ttagttggaa
    29821 gttgtagaaa aagacaatta gccatagctc ataatggaaa tctagttaat gcaaattatt
    29881 taaaagatat gctagaagaa gatggatata tgttccaaac taattcagat acagaagtga
    29941 ttttatatat acttgctaga tattacaaag gggatatagt agaaagttta aaagtgacaa
    30001 tggattatat aaaaggtgca tatgctcttg taataatgag tcaagaagaa ttggttgcag
    30061 ttagagaccc acatggattc agacctcttg tacttggtaa aaagggtgat gaatatatat
    30121 ttgcatcaga aaattgtgca atagatatac ttggcggaga agttataaga gatgtagagc
    30181 caggagagat aatagtagtc aaagatggag aactaaaatc atatttttat tcagaaaatt
    30241 ataaaccagt taagaaaagt tgtatatttg aacacatata ctttgcaaga aatgatgcaa
    30301 ctatagacaa tgtaaatgca tatgaattta gaattaaatg tggtgagaga ttagctcaga
    30361 atgaaactgt aaaggcagat atggtagttc cagttccaga ttcaggttgg ccaggagcta
    30421 ttggttatgc aaatgctagt ggattaaaaa taagtgaagg cttagttaaa aatagatatg
    30481 taggaagaac atttataaaa cctacacaag aagaaagaga aatagcagta aaaataaaat
    30541 taaatcctct ttcaacaata ataaaaggaa aatctataat tttagtagac gactcaattg
    30601 taagaggaac tacatctaaa caattagtaa aatctttaag agaagcagga gcaaaagaaa
    30661 ttcatcttag aataacttct cctccagtag cttactcttg ttactatgga atagatactc
    30721 caaatcgctc taaattaatt gcatcaagta acaacgtaga agaaatgaga gaatatattg
    30781 gatgtgatag tttaaaattc ctagatatag aaggaatgtt agatgcaaca gaacataagt
    30841 ctactttctg taaagcttgt tttgatggag aatacccagt taagaaaatt gataaggagg
    30901 aactactttc atgttaacat ataaagaatc aggtgtagat atagatgaag gaaatagagc
    30961 agtagacctt ataaaaggta aaataaaagg aacttatgat ggaaatgtag ttggagattt
    31021 aggtaacttc agtggattat acagtttaaa agattttgta ggtatgaaag aaccagtttt
    31081 gcttgcatct acagatggtg ttgggactaa gttaaaaata gctcagatga tggacaagca
    31141 tgatactgtt ggaatagatt tagttgcaat gtgcgtgaat gatttaattt gtcaaggagc
    31201 aaaaccatta tttttccttg actatatagc tcttgggaaa ttagtgcctg agcatatcga
    31261 aaaaatagtt ggtggaattg cagatggttg taaaatgtct ggttgtgcac taattggtgg
    31321 agaaactgct gaaatgcctg gaatgtatgg agaagatgac tatgatttag caggattttc
    31381 tgtaggaata gcagacaaag aaaagattgt ttctggaaac aatgttaaat caggagatgt
    31441 attggttggt atatcttcaa gtggagtaca cagcaatgga ttttctttta taagaaagat
    31501 atttttagaa acttacaatt ataaaatgga acaatatgtt gaagaactag gaatgacagt
    31561 tggagaagca cttttaacac caactaaaat atatgttaaa ttagctttag atgtattagc
    31621 taaacatgac ataaaagcta tagctcacat aactggtgga gggcttatag aaaatataac
    31681 aagagttata cctaaagggt taggactaga tatcaataaa aaatcttggg agaagcctcc
    31741 tatatttaaa atgatagaag gttttaatgc tgttgatgag agagaacttc ataagagttt
    31801 taacatgggt attgggctag ttttaatagt agataaagaa aatgctgatg atgttgtaaa
    31861 ctttataaat aatagagaaa atgacaatgc tgcttatgta gataaaaaat acagtgaatt
    31921 attagaagat aaagcgtata taattggtga agtagtagat agtcatgaag gtgtagaatt
    31981 atgctaaata taggagtttt aatatctggt ggaggaacaa atttacaagc agttatagat
    32041 ggcactgagt ctggagaaat aaaaggtcag gttaaagtag ttatctcaag taagcaaggt
    32101 gcttatgggc tagagagagc taaaaatcat aacataaaag caatttgtga aactgatgaa
    32161 gacaagataa tagagattct taaggaaaat aaaatagatt tagtagtttt ggctggttat
    32221 ttaaaaataa tcagcccaaa gctggttaat gagtttagaa acaaaatgat aaacatacat
    32281 ccatcattaa ttccttcatt ttgtggagct gggttctatg gagaaaaagt tcatcaaggt
    32341 gttatagatt acggagctaa agtaactggt gctacagttc attttgttga tgaaggtgct
    32401 gatactggac caatcataat gcaagatgta gttaaggtaa atcaagatga tgatgcaaag
    32461 acacttgcaa aaagagtgtt agaagttgaa cataggattt taaaagaaag tataagtctt
    32521 ttttgtgaaa ataaattaaa acttcaaggt aggagagtgt ttataaatga gtaagagagc
    32581 tttaataagt gtaactgaca agactggtgt agtggaattt gctaaggagt taaataagtt
    32641 agattatgaa attatatcta caggaaatac atttaaaaca ttaaaagaaa atggtgttaa
    32701 tgtaatgcaa gttgaagatg ttacaaattt tccagaaata ttagatggga gagtaaaaac
    32761 attaaatcca tatatacatg gtggtatact ttataaaaga gataaagaat ctcatgtaga
    32821 aactgtgaat gagcataaaa tacattcaat agatttagta gctgtaaatc tatatgattt
    32881 tgaaggaact ttaaaagctg gaaaaagtca tgatgaaata attgaaaata tagatattgg
    32941 tggaccatct atgatacgtt ctgctgccaa aaactataaa gatgtaatag tagtagtaga
    33001 tataaaagat tatgattcta taatagaaaa attaaaaaca gatacaatga cattagaaga
    33061 cagaaaaaaa ttatcatata aagctttctc aactacagga agatatgatg cgttaatatc
    33121 tagttatttt gctggagaag ttggagatac atatcctgac atattaaatt taactttcca
    33181 aaaagaacaa actttaagat atggagaaaa tccacatcaa aatggtttct tatattcaca
    33241 atcaaatgca aaaaatccaa tcttaaatta tgagcaatta ggcggtaaag aactttcttt
    33301 taacaattta aatgatttac atggatgttt agaagtaatg agagaattta aagatagtga
    33361 agaagtagtt tcagtagcta taaaacatgc taattcatgt ggggtaggtc taggaaaaga
    33421 tgcttttgaa gcatatacaa aatgttatga agcggataag gtttcaatat ttggaggaat
    33481 agtaggaata acttctacta tagataaagc tacagcagag aaattaaatg aaatattctt
    33541 agagatagta gtagcatatg attttgagcc agaagcttta gaggtactta aacaaaagaa
    33601 aaatttaaga atattaaaat tagctaagat agaagatagt ttacaaccat atgaaatgaa
    33661 atatttagat ggaaaattat taatacaaga tagaaataat atattagcag aaaaaagtga
    33721 aaatgtaaca aaagcaaaac caacagatgc acaacttaag gacatggaat ttggtatgag
    33781 agtagtaaaa aatatgaaat caaatgcaat tgcgatagta aaaaatggtc aaactttagc
    33841 tttaggttgt ggacaaacat caagaatttg ggcattaaaa aatgctcttg aaaataacaa
    33901 agataaagat tttacaggag ctgttttagc gtcagatgca tttttcccat ttgatgattg
    33961 tgtaacatta gctcatgaat atggaatatc agcagtagtt caaccaggtg gctctataaa
    34021 ggataaagat tcaatagaag cttgtgataa gtatgatatg gttatggtat ttacaggaat
    34081 aagacacttc aaacactaag gaggatatta tgaagatatt ggttgtaggc ggtggcggaa
    34141 gagagcatgc tatatgctgg aaactaagca aagaaaaaaa tgtagaaaaa gtttattgcg
    34201 cgccaggtaa tgcaggaatt acaaatgtag cagaatgtgt aaatataggt gatactaata
    34261 tagaagaact tttaaaattt gccaaagaaa atgaaatagg tctgacaata gtaggaccag
    34321 aagtaccact agttatggga atagtagatg agtttgaaaa agaaggatta agagtatttg
    34381 gacctaataa aaaatgtgct caacttgaag gaagtaaagc tttttcaaaa gaatttatga
    34441 taaaacataa catacctact gcaaaatata aagaatatac aaacttagaa gaagctatat
    34501 cagaaataga tagttttgga tatccagtag taataaaagc ggacggatta gctgctggaa
    34561 aaggtgttgt aatacctgaa aatagagaag atgctatagc aactttaaaa gaaatgatgt
    34621 cagataagaa gtttggagct gctggagata aaatagtaat tgaagagttt ttaaaaggaa
    34681 tagaaacttc aattctagca tttgtagaca atgatacaat agttcctatg gcaagtgcaa
    34741 aagaccataa aaaagtaaac aactatgagc aaggtccaaa cactggggga atgggaactt
    34801 tttcacctag tgaaatatat acagaagaat tagcaaataa agtaaaagag acagtattag
    34861 aaaaaacttt agaaggcttc aaaaaagatg gacttaattt taaaggtatt ttatttgttg
    34921 gtcttatgat aactgaagat ggagaaaaag tacttgaata taatgtaaga tttggagacc
    34981 ctgagacaca atctgtttta tttagattag aaacagattt acatgagata atggaaacta
    35041 tactagataa taagttaaaa gatatagaaa taaactatag tgatgatgaa gctgtatgtg
    35101 ttatgcttac atcaggaggg tatccagaca gttatgaaaa agggaaaatt ataactggtc
    35161 ttgaaaactt ggatgatgat atagtagtat tccatagtgg cactaagatg cttgatggta
    35221 atttagttac caatggagga agagtaattg gtataactgc aaagtcaact acagttaagg
    35281 atgcagcaga aaaagtttat gaaaacataa agaagataaa ctttgaagga atgcactata
    35341 gaacagacat aggaagataa tttttctaaa atggaggata aggtatgttg aatactgaaa
    35401 ataaagactc aatggttaga agagtattag ttgaaaaaag agaaggtttt gaccttgagg
    35461 ctaaagcttt aaaaaaagac ttagtagaaa gtttacacat agataatata gaaaatttaa
    35521 gaatattaaa tagatatgat gtagaaggaa tatcagaaga agtttatgaa aatgcagcaa
    35581 agacaatatt ctctgaacca aatttagatg tagtatatta tgaagaaata ccaaaattaa
    35641 atgatgaaag agtatttgca atagaatttc ttcctggtca atacgaccaa agaggtgatt
    35701 gggcagctca atgtgtacaa attgtaaatc aagggataag acctgctatt aatacagcaa
    35761 aagtttatat attatcaggc aaaataactg atgaagagtt ttctaaaata aaagattact
    35821 gtataaatcc agtagatagt agagaagcat ctttagaaaa accagaaaca ttaaaaatgg
    35881 aaactgaaat accaacaaca gttgaagtat tagatggatt tatagattta gatgaaaatg
    35941 gattaagaac atttgtaagt gaaaaaggat tagcaatgac tttaggagac ttacaacacg
    36001 ttcaaaaata ctttaaagat acagaaaaga gaaatccaac tataacagaa ataaaagtat
    36061 tagatactta ttggtctgat cattgtagac atactacttt tatgactgaa atagaaaatg
    36121 taaagataga agatggtaag tttaatgata tagtaaaaga agcgtatcaa atgtatctta
    36181 actcaagaga caatgtatat gtgaacagac ataaagatat atgtttaatg gatatagcca
    36241 ctgttgcagt aaaagaatta aagaagaatg ggaaattaaa tgacctagat gaaagtgaag
    36301 aaataaatgc atgtagtata aacgtagatg ttgaagttga tggtaagatg gaaaaatatc
    36361 tagtaatgtt taaaaatgaa actcacaacc atccaactga aatagaacca tttggtggag
    36421 cagctacttg tttaggtgga gctataagag acccattatc aggaagaagt tatgtttatc
    36481 aagcaatgcg tgttacagga agtgctgacc caagaacgac tttagaagat actttacctg
    36541 gaaaacttat gcaaagaaaa ataactacag aagctgctca tggatatagt tcatatggta
    36601 accaaatagg tcttacaact ggacaagttg cagaagtata tgatgaaaac tttgttgcaa
    36661 agagaatgga aataggtgca gttatagcag cagtaccaaa agagaatgta gtaagagaaa
    36721 gacctgaagc aggagatgta atagttcttc ttggaggaaa aacaggaaga gatggttgtg
    36781 gaggggcaac tggttcatct aaagaacata gtgaagagtc aatcttaact tgtagtgcag
    36841 aagttcaaaa aggagatgct cctaacgaaa gaaaaataca aagattcttt agaaataaag
    36901 aagttgctca aatgataaaa agatgtaatg attttggagc aggtggagtt tgtgttgcta
    36961 taggagagat tgctgatagt ttagatataa acttagactt agtacctaaa aaatacgatg
    37021 gacttgatgg aactgagctt gcaatttcag agtcacaaga gcgtatggca gtagctataa
    37081 aaaaagaaaa taaagataaa tttatacaat tagcagtaga agaaaactta gaagctacac
    37141 atgtagctac agtaacagat acaggatatt taagaatgtt ctggaatgga aaagcaattg
    37201 tagatataaa tagagagttc ttagatacaa acggagtaaa acaaactact gatgtccatg
    37261 ttactaaagt agatgaagaa aatacattct ttagctctaa tgaaatagta aaagatgtta
    37321 agtgtgcaag tatgaaagac aagtttacaa aagtattatc tgaccttaat gtttgttctc
    37381 aaaaaggatt agtagagatg tttgataata caattggtgg taatacagta cttatgccat
    37441 ttggaggaaa gtatcaagcg actccaacac aaggtatggt tgctaagata ccagtacttg
    37501 gaggagaaac taatacttct acaataatga cttatggata taatccaaag gtaggaaaat
    37561 ggagtccatt ccatggtgcc ttgtacgcag tagtagaatc tgtgtgtaaa ttggttgcta
    37621 ttggaggaaa ttatagtaca acaagactta cattccaaga gtattttgaa aaacttggta
    37681 acaatccaga aaaatggggt aaaccattct cagcactttt aggagcattt tatgcacaaa
    37741 gtaaatttga gatacctgct ataggtggaa aagacagtat gtctggtaca tttaaagata
    37801 tcgaagttcc accaacatta gtttcatttg ctgttgatac agtagatgcg aagaaagtag
    37861 tatctccaga atttaaaaaa gcagactcaa aagtagtaat gttatgtgta aacaaggcag
    37921 aaaatgatgt tgtagatttt gaggaattaa agagaaactt agacaaagta agagaactta
    37981 tacatggaaa taaagtttta tcaacatatg cattaggatt tgcaggtgta ggtgaagcaa
    38041 taagtaaaat ggcttttggt aataaaatag gatttaagtt tagtgaagaa gctgaaaaaa
    38101 catttacaga tgataaatta tttgaggcta gttatggaaa tatagtactt gaattagcaa
    38161 atgatgattt atgtatgtta gaaggataca attatgttgt gcttggaagt acaactaaag
    38221 aagctagtat atttatcaaa ggtgaagaac ttgcattaga tgaattatat aaagcacact
    38281 gtagtacttt agagcctata ttcccaacta aaacagaaga agtaaaatca aagatagaga
    38341 ctataagtta tatatcacaa ggagaagcta aaaaatcatc attaagtata gctactccaa
    38401 gagtatttat accagcattt cctggaacaa actgtgaata tgactctgca agagcatttg
    38461 aaagagtagg agctaatgca aacataagag tattcaagaa tttaacttat aaagacatag
    38521 aagattctat agatacaata gtaaacgaaa taaaatcttc tcaaataata atgttaccag
    38581 gtggatttag tgctggtgat gagccagatg gttctggtaa atttatagct actgtattta
    38641 gaaatcctag agtgcaagaa gctataaatg aatttttaac tcaaaaagat ggactaatgt
    38701 taggtatctg taatggattc caagtattaa ttaaattagg attagttcca tatggagaaa
    38761 taagagttcc aagtgaaagt gctccaacac ttacttacaa taatattggt cgtcatcaag
    38821 ctaagattgc tagaactaga atttcatcta ataaatctcc ttggttagct caaactaatg
    38881 ttggagatat tcataatata gcaatttctc atggagaagg taaatttgtt gctagtgaag
    38941 atgttatgag agaattaatt gctaatggac aagtagcaac tcaatatgtt gactttaata
    39001 atgaagctac ttatgatatt gagtttaatc caaatggttc attctatgct gttgaaggta
    39061 taactagtgc tgatggtaga gtgtttggta aaatgggaca ttctgagaga attggtgagg
    39121 aagtatttaa gaatattata ggtgaaaaag accaaaagat atttgaatct ggagttaaat
    39181 attttagata aagctttgat gtttaattga tagctagaat atataattta gatatttaga
    39241 tttaaagaga tgtaataata tgatgataat catttaatat tacatctctt ttttatttat
    39301 ctaagaaata ataagttcaa cctaagttaa aatacaagct gtgttataat taattactaa
    39361 gtaaaattaa atatttattg gagggcaaca taattaatga aaatattaat aacagggtct
    39421 aatggtcaat taggaaaaga attggttagc caattagaag caatcaatca aagtataaat
    39481 caaccaaaat acgttatatt agctacaaca agaaatgatt tggatatatc caatcagact
    39541 aatatagata attttatttt gcataataaa cctgatgtaa ttgtaaactg tgcagcatat
    39601 acaaaggttg atgcatgtga agataatatc gaaattgcgt ataaaataaa tgccttagga
    39661 gtgagaaatt tagcaatagc atcagaaaaa gttaatgcta agcttgttca catatcaact
    39721 gactatgtat tcaatggttt ttctaaatat ccatataggg aagacaataa aactgaaccc
    39781 aattctgtgt atggaaaaag taaacttatg ggagaaaagt ttgttgaaca attctcacat
    39841 aagtacttta tattgagaac agcatggtta tatggtaatg ggaataattt tgtaaagact
    39901 atgataaaac tttctttaga aaataaagag gttaatgtgg ttaatgacca gtttggaacc
    39961 ccaacaagta cagtagactt agcaaaaatt ataataagaa tcatggaaac agaacattat
    40021 ggagtttatc atgctacatg tgaaggtgag tgtagttggt atgattttgc caaaaagata
    40081 tttgaattaa aaaatataga tataaaagta aatccaatta agagtaacga atttaaatgt
    40141 aaggctcaaa gaccactata ttctgtattg gataatttta tgttgaagtt aattggttta
    40201 aattctttta gaaagtggga agaatccatt gaagaatatt tagaaagata tgatataaat
    40261 gaaaagtaaa tgtattattt ttagaaaaga aaataagata ttataaaaaa tataaatatt
    40321 tcttttattt atccgataat cataatatga caaaaacaaa tttaaggatg gaaaaagatg
    40381 aaaggaatta ttttagcagg agggtctggt acaagactat atcctataac aaaatgtata
    40441 tcaaaacaaa cacttccaat atacgataaa ccaatgatat attaccctat gtcggttctt
    40501 atgttggcag ggatacaaga tatattaata atatctacac caagagatat aaaagcattt
    40561 gaagaattat ttggagatgg aagttcacta gggcttaaaa taacatatgc aattcaggag
    40621 aaagcaaatg gaattgcaga agcgtttata ataggagaag aatttatagg aaatgacaga
    40681 gtgtctcttg tattggggga taatatattt tatggatatg gatttagtga aaggttggag
    40741 aatgcagtta aaagaaaagg agcaactata tttgggtatc atgtaagtaa tccaaattcg
    40801 tttggagttg tagaatttga taaggatttt aatgtattat caatagaaga aaaacctaca
    40861 gttcctaaat ctaattatgc tataccagga ttatattttt atgataatga tgttgtagag
    40921 atagcaaagc tagttgaacc atcagataga ggtgaattag aaataacctc tataaacagt
    40981 gaatatctaa aacgaggaaa actaaaagtg gaacttctag gaagaggtat ggcttggctt
    41041 gatactggta cacataaagg tctattggat gcttctaact atgttgaagt ggttcaaaca
    41101 agacagggac tgtatatagc atgtttagaa gagattgcat atacaaaagg atatataagt
    41161 agagaacaat taatgaaact agccaagcca ctgataaaaa ctgaatatgg tcaatatcta
    41221 ttaaaattgg ctagtgaaga tatgcaatat ggtagataaa atttattata aattttatta
    41281 agggagtttg ttataatggg aaaaatcaat gttataaaga ctaaaataga aggattatat
    41341 atagtcgaac caaatgtatt ttgtgatgaa agaggatatt ttatggaaag ctataataaa
    41401 aaggatttta tagagcaggg tcttgatatg tgttttattc aagataatga gtctagctca
    41461 agaaaaggtg ttttaagagg aatgcatttt caggttagat atcctcaagg aaaattagtc
    41521 agagttacag agggagaagt tttcgatgtt gcagttgatt taagaaaaga ttcacagaca
    41581 tttgggaaat gggaaggtgt aatattgtct gataagaata aaaaacaatt ttatattcca
    41641 gaagggtttg cacatggatt tttagtttta tcagaaaaag cagtatttaa ttacaaatgt
    41701 acaaactatt attatccaga atttgagcat gggtttatat ggaatgacaa gcaaatatct
    41761 attgagtggc ctttaaaagg aattgaagaa atatttttat cagaaaagga caaaaaacaa
    41821 aaaagtttta aagatatttg tataagacgt taataaaaat ttattagaat taacatttag
    41881 gagaaaaaat atgaaaaaaa tactgataac tggaggagca ggatttattg ggagtaactt
    41941 tgtacattat atgcttaaca agtatgtaga ttattttatt gttaacctag acttgttgac
    42001 ttatgctggt aatttagaga cattaataga aatacaagac agttccaatt ataaatttgt
    42061 taaaggagat atttcaaata gggaacagat gtataagcta tttgaagaag aaaggtttga
    42121 tatagttgtt aactttgccg ctgaatctca tgtagatagg tcagttgaga atcctgattt
    42181 atttattaaa acaaacatta taggaacaca agtactttta gatgcatcta taaaatatgg
    42241 agttaaaagg tatcatcaaa tatctacaga cgaagtgtat ggtgatttac caattgatag
    42301 aaaggattta ttctttacag aacaatctcc tatcaatcca agttctccat attcagcttc
    42361 taaagcatca gcagatttgt tggtaagttc ttattataga acatatggat tgttaacaac
    42421 tatatctagg tgttccaaca attatgggcc atatcatttt cctgaaaaat tgatacctct
    42481 tatgatttta aatgctttgg aaaacaaaca attgccagta tatgggaatg gggaaaatgt
    42541 aagagattgg ttacatgtat atgaccattg tactgctata gacttaataa tacataaagg
    42601 gaatataggg gagatttaca atataggagg tcataatgaa agaagtaatt tagaagtagt
    42661 aaaaatgata ttaaatcttt tagataaatc tgaagagtta atatcctatg taaatgatag
    42721 acctggtcat gatttaagat atgctattga tgcaactaaa atagagaatg agttaggttg
    42781 gaaggcaaaa tatgactttg atttgggtat aaaagaaact gttaagtggt atatagaaaa
    42841 tgaatcttgg tggaaagctg ttttatcagt gtaactttga aatttataaa atttagaaag
    42901 gggcgtttta tattgaatga gataagttca ctaattaata tattagcatt gactcaatta
    42961 tctggtagta atactacaaa tcaatgtaac tgtacatata atagttcatc tggatttgat
    43021 atgattatga tgaccttgtt aaaagcatta tcacaaaata atcaacagac ttctaatggc
    43081 tacaatttat cgtctaattc tgagaattta tttgacgaac tcggcagtgt agtaaataat
    43141 acagctagta gatttataga tgttgataca aaagataaaa gtgtaaaatc aagaattgaa
    43201 aatgctatag agcaagcatc aaaaaaatat aatgtggatt cgaatcttat aaaagcaata
    43261 attaaagttg aatcggattt taatccaaat actgtttctt ctgctggtgc taagggtctt
    43321 atgcaactta tgccagaaaa ttgcagagac ttgggggtta cagacccttt taatatagag
    43381 cagaatatag atgctggaac tagacatatt aaggaataca ttgatatgtt tggagggagc
    43441 atagaaatgg gattgatggc atataatggt ggtcctggaa gaatgaaaag tagaggagta
    43501 gaatcaatct ctgacctgta taaaatgcca aaagaaacac aaaattatat tcctaaggtt
    43561 atgaaatatt atagagggta ggctttatta tggggataaa tatagataag ctaaacataa
    43621 gtccaagtat aaatatggag gttaaaaaac taaaagagag taataaattt tctgacaaat
    43681 taaactcttt aaaacaatct tcttcattgg aagatataaa acgaaatttg gaaagtgtaa
    43741 aaaaggcagg agaaaggctt gtacttactc aaaactatgg cgacattagt atatataaaa
    43801 atgcagtaaa agaataccta aaatctgttg tagataatat gtattcactt gataaaaata
    43861 gtagcttttg ggagcatcag tattataaga atgtaaagat tgttgatagt aaacttgagg
    43921 atatgactag taagttaatt agtgaagaaa aagaaaacat agatattgtt tctactgttg
    43981 atatgataca aggcttgttg atagatatgt atagataagg ttatacaagg agaaaagttt
    44041 tatgaatagt aaaaaagagg atatatatga attgaatttt gggcaattaa tagaagaaaa
    44101 atctggtgtt atagaagaaa atcaaaatgt ttttaatgca aaagtaaatg tagctgtttc
    44161 tataggaaat acaacagaaa caataaaaaa tatattagat ttaaaagaag gctctataat
    44221 aaaactaagt aagaatgtgg atgaaaaatt agatatatat gcaaatgata gattgtttgc
    44281 ttatggagaa agcattatta caaatgagaa gttatctgta agattatcta aaatagaaga
    44341 ttcagatgta aattagtatt ttaaatagta tccctttagg ctatgatgta tgagtttagc
    44401 taaggggata tttttataaa aaatttaaaa tatgcgtgaa ttatataaat gtttttattt
    44461 tatgagcgat aattaaatta taagtatgta attttggagg tgaaactatg gatattaaaa
    44521 atgtaagttc aaatattgta gtgaataatt atttaaagaa tacatctggt gtaaaaaaag
    44581 tggaagaagt taaaaaaatt aataaaacag acaagataga gctatctaaa tttgcaaagg
    44641 aaatggcaag tgtgtcaaat atgagtgatg agaatattag gaataaaaaa gtcgatgaaa
    44701 taaaaaatgc attagaaaat ggaacttaca aaataaaacc aaaagaaatt gcgaaaaaaa
    44761 tgatagagga tatgcgagga taaagtcatg attacagact taaaagttat aatatatgaa
    44821 gaaaaaaata taattagtaa gatgatggaa ttattaaaag aacaatttga ttatatagtt
    44881 gaaaaagact tagaaaattt aaatatgtta aatcctaaac tagaagatat tagcagggag
    44941 cttgcatcca tagaaataaa gagaagacaa ctttttggtg atgatatttc aatgagtgaa
    45001 gttgttgaaa atagtaatga tagatattta aaagaaatat atacagactt gaaacacatt
    45061 cttgctttag ctaaaaatca acaagaatca aatgattctc taataaagaa agaacttatt
    45121 tttacgaaaa aaatgattaa tttcataaaa ccagctgata agcaaactgc aacttataat
    45181 tcttatggaa atattagaaa ataaatatta ggaggagaaa tatggcagga ttattttcca
    45241 ttttaaatac agctaattca ggaatgaatg cgcaccaaaa agcaatacag acaatttctc
    45301 ataatataag taatctagat acagacggtt attctaggca aaggactgag tttgctacaa
    45361 atagtccaat gtatatgcct agtttgagta attcaattgc aagaggacaa ttagggactg
    45421 gagtacacgt aacggatgta acaagagcta gaaatagttt ttatgattac caatttagag
    45481 cagaagctca taagtatggt aatacagttt caaagtatga ctattataat actatagaaa
    45541 ctataataaa tgaaccatca gactatggaa tatctgcagg tatagacgac ttttttaaag
    45601 gatgggaatc attagcaaag gatataaata gtgatagtaa aaaaagtcta gtggtagaaa
    45661 atgcatcaaa tctagcaact ttaataagtg aaagttataa gaagcttaat aagttaaagg
    45721 gagatataga cgataatata aactctgaaa taaaacagat aaatgacatg ttaaaaagtc
    45781 ttgaagattt gaataaatct attgatgtta tatctggttc tggctctact ccaaatgatt
    45841 tacttgatga aagagataga atattagatg acttaagctt taaattagac cttgaaaata
    45901 gcgatgttaa aaatatgctt tcagatggta aattagaact taatgaatta aaaaatgcag
    45961 atggaacttg gaaaacaggt gtatcaggaa ccttacaagg cttgtttgaa atgcatggta
    46021 agattgatac atataaaagc gatttaaaag atgtatctga tgggttagct aagcagataa
    46081 atgatgttta taatacatct gctggaataa cagtaacaga tttttttata acttcaaatg
    46141 tagatggtga agatatcata aaggtaaatc cagctataaa gagtaaccca aatgaattaa
    46201 aacttactgc tgatgaggct tcaaagattg ctaagttaaa ggatgaaaaa atagatattg
    46261 gggtggctgg tggaaaagta agtacaataa gtgaccacta taaagccttt gcagaatcag
    46321 taggtttaga ttctcaaaaa gtgaatcaag atgaagtgaa tcaacgtaaa attataaata
    46381 atattgataa ctcacgaatg agtgtttcag gcgtatcttt ggatgaagaa atgacagagc
    46441 ttataaaaat acaacgctcc tatcaagcta gtgcaaaagt tatgtcaaca gctgtgcaac
    46501 tgttggatgt tgtaataaat ggaataattt aatagaaggg ggaatttata atgagagtta
    46561 gtacaggtat gatgtctagc aactatctaa actcactaca agataatcta caaagattag
    46621 ataaggttaa tagacaagtt aatacaacga aagaaataaa caagctatca gataatccat
    46681 ataaggcaat aaaaatattg aactcaaaaa gtgaaataaa aactatggag acttatgttg
    46741 agaattgtaa agatacaggg aattggttag aaactactga tactgcatta gatcagcttg
    46801 gaaaattatt atcggacata aagaaaactt tggtggcaag tggtgatggt gcatattcag
    46861 atgatgagct aaagactctt agtaatgaaa ctaatgaaaa gatgaaagaa attgcaaatg
    46921 ccttaaatgc aacacatgaa ggaaagtaca tattttcagg ttcaaataca ggtgtaccac
    46981 ctgttgaatg tatagataat gctgacggtt cagtgagctt aaagtttaat tctagtttaa
    47041 atcttaataa attaaatgac tctttgagta tggatatagc tcaaggaata agtgtagatt
    47101 ataatgttaa attgagtagc cttggatttg acccaacaaa gactgaccca tttgaagagt
    47161 taaataatat atcaaagaaa ttagcaaatc cagatgctga tgctataaaa gagctgacaa
    47221 caacttgttt aggcgacatg gaacaattaa tagaaaatac agtaaatgtt aggtctatat
    47281 atgggacaaa agcaaataca gtagaggcta tgaaggaaaa aaatgatgat agtttgatac
    47341 agttaaagga tattttgtca caaaatgaag aaatagatta tggagaaaaa ctcgttcaat
    47401 taaaggcagc agagttaact tatcaagctt cactacaaac aggaggaaag ttatttaatg
    47461 tatctatatt agattacata tagtaaaata acgcagatat gatgaaggtt acattaaaaa
    47521 aaggtatatt aggttttgaa aatttaaaag aatatgagct tttagatata gaagataatg
    47581 attctttaaa agaatttaat tctactgaag aagaaggtat aggatttgtt gttgtatctc
    47641 catttgagat tatcaatgaa tatgaaatag ttttaaatca ggaaacaata gaaaaactag
    47701 aagtgaaatc tccaaaggat gttgtactat ttaatataat tactcttggt cagacatttg
    47761 aagaatcaac tgtgaacatg aaagctccta tagtgataaa tataaaaact aattttggaa
    47821 tgcaaataat tttacaggat gataaatatt caataaggca tccattattg agaggtgata
    47881 atggatgcta gtaatttcaa gaaaaaaaga tgaagcagta cttataggag aaagcataga
    47941 agtaaaaatt gtaggggtag atggaaacaa tgtgaaacta gctatatcgg cgcctaacaa
    48001 tataagtata ttaagaaaag aaatatacga aaaagtaaag agtgagaata taaaagctac
    48061 aaataaaaat ataaaaattt taaagtcatt aaaataataa gcttggagga agtaatatat
    48121 gtatggagaa aatccttata attcttataa acaaaatgct gtttttatgg catcaaagga
    48181 acaattgtta cttatgcttg ttgatggagc agtaaaatat accaaaatag ccagagcagc
    48241 tatcttagat aaaaatacac agaaagcgca tagagaactt gtaagagttc aggatatatt
    48301 tacagaacta atggttacat tagaccaaaa tgcaggacaa tgggcaaagg atatgtacaa
    48361 agtttatgat tttgtaagat atgaactttc aagagccaat attagaaaag acatccaggt
    48421 aatagataat gtgttgcctg taatagaaca aattaaagac acatggcatg aagctgataa
    48481 aaaaagtaaa gaagaaagaa gtaggtacaa ataatggaat taaatttagc tgaattagat
    48541 aagttatcaa aagaggaatt actattgatg cttgtagatg gcacagtaaa atataccaat
    48601 atatccaaag aagctctttt aaataacgac tatttgaaag cacataatga acttataaga
    48661 gttcaaaata tatttacaga actaatgact acattagacc aagatgcagg tcaatgggca
    48721 aaggatatgt ataaagttta tgaatttata aaatctgaat tagcaatagc tgatgaaaat
    48781 aaagatatta aaataattga tgatatattg ccaatagtaa aacaaattag agatacatgg
    48841 catgaagttt acaatcaact aaaaatataa ttagatggag ggataaaggt atgtcaagta
    48901 taagtcctat aagagttaca ggtctttcag gaaattttga tatggaaggc ataatcgaag
    48961 ctagtatgat tagagacaag gaaaaagttg ataaagcaaa acaagaacaa caaatcgtta
    49021 aatggaagca agaaatatat agaaatgtta tacaagaatc aaaagatctt tatgataaat
    49081 atctaagcgt aaattctcct aatagtatag taagtgaaaa agcatactct tctacaagaa
    49141 taaccagttc tgatgaaagt attatagtag caaaaggctc agctggtgca gaaaaaataa
    49201 attatcaatt tgcagtttct caaatggctg aaccagcaaa atttactatt aaattaaatt
    49261 caagtgaacc tattgttcga cagttccctc caaatgccag tggagctagt tctttaacta
    49321 taggagatgt aaatatacca atatctgaac aagatactac aagtactatt gtaagtaaga
    49381 taaactccct ttgcgcagat aatgatataa aggcttctta tagtgagatg acaggtgaat
    49441 tgattatttc gagaaaacaa actggttcgt catcagacat taatttaaaa gtaattggaa
    49501 atgacaattt agctcagcaa attgctaatg ataatggtat cacatttgca aatgatgcta
    49561 gtggaaacaa agtggcaagt gtatatggaa aaaatctaga agctgatgta actgatgaac
    49621 atggaagagt aactcatata agtaaagaac aaaattcatt taatatagat aatattgact
    49681 ataatgtaaa ttcaaaagga actgcaaagt tgacttctgt cactgatact gaagaagctg
    49741 ttaaaaatat gcaagcattt gtggatgatt ataataaact gatggacaag gtctatggtt
    49801 tagttactac taaaaaacca aaagattatc cgcctcttac agatgcccaa aaagaagata
    49861 tgacaactga agaaatagaa aaatgggaaa agaaagctaa agaaggtata cttagaaatg
    49921 atgatgagtt aagaggtttt gttgaagata ttcagtctgc attttttgga gatggaaaaa
    49981 atattattgc attaagaaaa ctaggtatca atgaaagcga aaattacaat aaaaaaggtc
    50041 aaatatcatt taatgcagat actttttcaa aggctcttat agatgatagt gataaggtat
    50101 acaaaacact agcaggttat tcttcgaatt atgatgataa gggaatgttt gaaaagctaa
    50161 aagatattgt atatgaatat tctggaagtt caacttctaa acttcctaaa aaagcaggta
    50221 tagaaaaaac tgcttctgct agtgaaaatg tatattcaaa acaaattgca gagcaagaaa
    50281 gaaatataag caggttagtt gaaaaaatga atgataaaga gaaaagactt tatgctaaat
    50341 attcagcctt agaatctttg ttgaatcagt attcttccca aatgaattat ttctcacaag
    50401 cacagggtaa ttaattatgg ataaattaaa agaaaaatta aatttatata aagatatatc
    50461 tttacaaatt ataaatttga ttgaaaagga agaatatata aagataagta gtaagttagg
    50521 tgaaagacaa gagattataa atagtgtaag tgaaattgat agaaatgatt ttatacagct
    50581 ttataataga atggaattaa ttgaaattga tagtaggata agagatattt tacaaggaca
    50641 attattagaa gtaaaaaaag aattacatga gtataagctt actaaacaag tgaacacaat
    50701 gtattataac ttaaatagag aaaaagtaaa tatttttaat aaaaaagtat aaagttatgt
    50761 accatcttgt ccgattatat aaatgaagaa ttaataaaaa agattaaaag tagaaatgac
    50821 aaggatgtca actatactaa ggagggtaaa ataatgagag ttaatacaaa tgtaagtgct
    50881 ttgatagcaa ataaccaaat gggaagaaac gtaaatgcac aaagtaagtc tatggagaag
    50941 ttatcttctg gtgttagaat taagagagct gctgatgatg ctgctgggct tgctatatct
    51001 gagaaaatga gagctcaaat aaaaggttta gaccaagcag gaagaaacgt tcaagatggt
    51061 atatctgttg tacaaacagc agaaggtgca ttagaagaaa ctggtaatat actacaaaga
    51121 atgagaactt tatcagtaca atcttcaaat gaaactaata ctgctgaaga aagacaaaag
    51181 atagctgatg agttgttaca attaaaggat gaagttgaaa gaatatcaag ctcaattgag
    51241 tttaatggta agaaattact tgatggaagt tctacagaaa taagattaca agttggagct
    51301 aattttggaa caaatgttgc aggaacaact aacaacaaca atgaaataaa agttgctcta
    51361 gtaaatactt caagtataat gagtaaagca ggtataacaa gttctacaat agcaagctta
    51421 aatgcagatg gaacttctgg aacagatgca gctaaacaaa tggtgtctag cttagatgta
    51481 gctcttaaag aattaaatac atcaagagca aaattagggg cacaacaaaa tagactagaa
    51541 tcaactcaaa acaacttaaa taatactata gaaaatgtta cagcagctga atcaagaata
    51601 agagatacag atgttgcttc agaaatggtt aacttatcta aaatgaatat attagttcaa
    51661 gcatcacagt caatgcttgc tcaagctaat cagcaaccac aaggagtttt acaattatta
    51721 ggataaaata attttgtata ttggatatag aagaaagggt aaggctttgc tttactcctt
    51781 tcttttttta caaatatact tttgaaagga aaatatatat gttacttagt atagttatga
    51841 ttgttaaaaa tgaagaaaaa atacttgaaa aaactttaaa atcattaacc acattaagaa
    51901 gttctataga gagtgaactt ataatagttg atacaggttc tacagatgat actataaata
    51961 tatctaggaa atacacggaa aatgtgtact ttcataattg gaataatgat ttttctagta
    52021 tgagaaatat atcaatatcc tatgctaaag gtgaatggtt acttatatta gatgcagatg
    52081 aaatactaat tgattcttca actattgtga aattttttaa agatggtctt gataaaaaat
    52141 ttaattcggc ctcaatacaa ttaaaaaatc tttactcata tgataaaaaa ttatatggat
    52201 attgctcagt gcttagactt tttaaaaaca tcgattttag ataccatggt aaagtacatg
    52261 aacaaccaat atacaaaaac cctatattta ataatgttgc tgattttgaa cattatggat
    52321 atttgtttga agatgaagaa ataagattaa ataaagtaaa aagaaatgaa aaactgttat
    52381 ttgaagaatt aaaagagaat gataattctc catatacaaa ttatcaatta gggaaaaatt
    52441 ttattatatt aggaaaatat catgaggctt tatactatct tgagaaatct tatgaattat
    52501 attctaaact agaatcggca cctggttact tgataacaag tcttgtaaaa acatatttat
    52561 atttaggaaa gcataagaag tgtgaaaaat tgtgtctaaa gtatattaag aaagacatga
    52621 ataatattga tatatattat tatattgcac aagcacaagt agatttaggg aagtatgaaa
    52681 atagtataga ttcgtataag agatatattt atttgttaga taattatgaa ataagtacac
    52741 aagcaaatag tttattttca gacactgata ctgtagggct tagggatgaa gctattatta
    52801 cactgataaa gatttattat aaacttgaaa aatatgagtc tgtaatttca gagtacaata
    52861 acattgagga tatggaaaag aagaagaatg tgtactttag cttatttatg tccctataca
    52921 aattgaacag atttgaagat ataaaaaact attataaaga agtttcaaat tctaaaattg
    52981 aaaaaaatag tttttataaa aatatagaag aagttataaa taatattaaa gatgatgaaa
    53041 aagagcatgt atataaaatc ctttgtgaca ttgaaggtaa ttatggcatt ttaaatcaga
    53101 taaggataaa taaatgtgta tctatagata agtgtaaaga aatactcaat gaagaaaaag
    53161 aaataattta tgcatatatt ataaaagtag cttttgaaga taagattgat ttactagata
    53221 ttttttatga aatggaatat gtatggatag agaaatacct aaaatatctt ttagcactag
    53281 aaagaagttt taatttagta ctatataagt acataattag tcgtccgaat acatcagata
    53341 taaaaaagat aagagtatat aaagctatat ctaaagtagt tttagaaagt ataagtttaa
    53401 gtgatgaaaa atataaggag attttttatc tttatataat gtattcttat aagtacataa
    53461 gatacatata tagaaattta agcgattttg aattaataaa atttgtttgc aatgatttag
    53521 ataagtttac tatagaattt aagcatctac ttgacattaa aaataaagta aatgatgcta
    53581 atgaaaaatt agagtatata catagtatga ggaaattact aaatgagtat cctttttata
    53641 ataaaataat aaaaatactt ataagtgaat tagaagaaaa tataaaagaa agtgaagaat
    53701 ttaaggattt aaaagatagt tttattatta gtatagaaaa tatgattgaa actggcaaaa
    53761 taaatgatgc gaaatcattg gtagatgact attcaaaaac atttatggat gatgtaaaaa
    53821 ttttaaatat aaagggaatt ttatatatgt ttgaaaataa gcttgataaa gctgatttta
    53881 tgttaaaaaa ggcattatca ttagatgtag aaaatgaaga tactatatat aatatagaat
    53941 acttaaaaag tttaagataa gtcaaaaatg ggagaattga agaatgaata caccaatagt
    54001 ggttaatgaa aaatctaaag atataataaa agattggcaa tatacgtata caaacgaaga
    54061 tataaaattt acaagcataa taatattgac atataatcaa atagaatata ctaaattatg
    54121 tattgaaagt attaggaagt ttaccccaaa gaataaatat gaaataataa taattgacaa
    54181 taactcttct gatggaacag ttgagtggtt aaagggacaa tctgacataa aatctgtata
    54241 taataacaaa aacctaggat ttccaaaagg atgcaatcaa ggtattgagt tggctactgg
    54301 tgaaaacata ttgttgttaa ataatgatgt aatagttact ccaaattggc tttataattt
    54361 gaataaagct ttatggtctc aagataatat aggtgctgta ggtcctatta caaatgcatg
    54421 ttcgtactat caacaaattg atgttgatta taaatcaatt gaggatatgt ttatgtttgc
    54481 acacaaaata aatacatcta atattaacct atggaaatat agattaaagt tagttggatt
    54541 ttgcatgtta ataaaaaaat ctgtgctgga taatgtaggg gttttagatg aattattcac
    54601 acctggaaat tttgaagatg atgatttatc ttttaggatg ctagagtcag gatataagct
    54661 attattatgc caggatactt ttatacacca ttttggaagt gtatctttta aagaaaatcg
    54721 taataaatat acagatataa tgtatattaa tagaagaaaa tttgaaacta agtggggatt
    54781 taatccagaa tatagtggaa tgataagaac agatttgatt tctaaaatag atgatagttt
    54841 ggaggcaaat attaatgttt tagaaatagg ctgtggtata ggggctactc ttttagaaat
    54901 aaaaaatata tataaaaatg catcaatata tggaatagaa attagtgagt cagcaggtaa
    54961 acttgcaaag tatagtgcag atgtacttat agcagatata gaaaaagtaa agttggatta
    55021 tgaaaaagat ttttttgatt atataatatt gggagatgta cttgaacatt taactgaccc
    55081 atggaagata gtcaatgaac tgaaacaata tctaaagaag gatggttgtt tgatatctag
    55141 tataccaaat attatgcatg tttctgtttt aagaaacctt gtgttaggta attttacata
    55201 tgaagattca ggaattttag acaagactca tttaaggttt tttacattaa atgagataac
    55261 aaaaatgttt aatgatagta attacataat aaatagtgtt acagctacta gtatgccact
    55321 ttcggaaatt gataatagat tcattgatac aatgtgtaag gaaagctgta aagaaatgag
    55381 acaacagtac gaatcttatc aatatatagt aaaagccact aaaagcatag atactaaaag
    55441 atattctagt attagtatgg ttaatttaaa atacgcttta atgagaattg ataatgaatt
    55501 agatgtagat agtaatttaa gttatatatt tgataattat aatataactg aaccatgttt
    55561 tgttgatgat attgaatatt tagttgatac gtttgtgata aataaagcaa ctctattaaa
    55621 tagattagga gttgaggcat ttaacagagg tttaggagat tttagtataa acatgtttat
    55681 gacagtatta gagatggata gagatgatat tgattcaata tataatataa caagtgtatt
    55741 atgtgaatta gaagaatttg atactgctta caacttaatt aaaaatagta gcaaaaaagt
    55801 acagagtgaa agtgacatac aagatatact ttattttatt gaggggaaga gatatgaata
    55861 atgataagat atgttttata acctgtgtaa atgatgaaag actttatgag gaatgtcttg
    55921 tatatataaa tagcctagac attcctaaaa actttagcat agaaacagta gctttaagag
    55981 gagccgattc tattgcctct gcttataata aagctatgag agaaacagat tctaagtata
    56041 aggtatactt acatcaagat gcttttataa ttaataaaaa tttcatatat gacattttag
    56101 agatatttaa aaaaaataca gatgtaggaa tgattggtgt agctggagta aaagatatac
    56161 ctgtgaatgg aatttggtgg gattctgata aaaaagcagg gaaagtatat gatagccata
    56221 caggtaaaat ggatatttat gaatttagta actttgaaga atgttttaat gaagttatgg
    56281 ctatagatgg attaattatg attactcaat atgatgtatt ttggagagag gataagtttg
    56341 atgggtggca tttttatgat ttgtcacaga gtatggaatt tagacaaaat aaatacaaag
    56401 ttattgtacc aaaacaaaat aagccttggt gtatgcatga ttctggagta gcaaatataa
    56461 ataatggata tgatgaatat aggataaaat ttgtaaaaga gtacataaat cttgatttat
    56521 atagagaaaa tttttatgaa tttgataaga agaaagcaag taccatactt aagagtaata
    56581 agcatgtaat ggattgtatg aaagaggagc ttacagaaca attaagatta aagaattata
    56641 aggatgtttt agatattgtt tctaaaattg ctcatttttc tgtttttaat catactggct
    56701 tgtattcttg tgaaaaatgt gagagagcat tgctagaatg tgcaaataat ttaccagaaa
    56761 tagaaaatac gaaattattt acaaaaaaag atttaaaaag aagaaaagta ttacatgttt
    56821 taagtgaagg atattcaaca ggagggcata caagactagt taaaaattgg attgagattg
    56881 acaaagattc tgttcattca gttgtaacta catggcaatc agatacactg ccagactggt
    56941 tattaaaagc aatagatgat agtggtggcg aagttatatc tttggattta atttcagatg
    57001 attatttaaa aagagctgta gctttgagga aaatagctta tgactgggct gatatagtta
    57061 ttttatatgt tcatatgtgg gatacaatac ctgttgttgc ttttggagtt gatgggaaaa
    57121 tacctatttt acatgttaat cactatgata gtactttttg gttagggaca agtattgcag
    57181 atattgtcat tgattttagc gaagctagtc aattaattac attagataaa agaggcatag
    57241 ataaatctga aatattacca atgccattaa aagaaaaaga agtattttca aaatctgaag
    57301 ttagaaaacg atataatttg gaggatgatg tgacaataat attgactatt gctagtgaaa
    57361 gaaaatacaa tagtgtaaat gatattaatt actttaaaat tgctaaaaca ttgattgaaa
    57421 atacagataa tacaattatt attgttgtag gagttgatac agagaatctc tattggaaaa
    57481 ctttaagtaa cgttacagat aatagagtta tatgttttgg aacaacagca aatatagacg
    57541 atttttataa gatttcggat atatacctag agtctttcat ggtagggagt catacttcaa
    57601 aattagatgc aattttatat gatttaattc ctgttaagtt taaaaatgaa gtttcaccaa
    57661 tttttacaaa tatgtggaat tttatagata agtttgatta taagaatata aaagaggtta
    57721 tagacatgat aagaaggtac aatactagtg aatttgacag ggataagata aaagaaaaac
    57781 aatcagaaat aaaacatttt gttatagata aacactgtat tgaaataaaa aactatatag
    57841 acaaaatata taaagaggct aatcatcata aagttaagta tgatttatat aaaaatgatg
    57901 aaatcgaaga ctatcagctt tttgttgcat tatatggtta tatggtaaaa aataaaaaca
    57961 tgtcataggt gataaaatga acaatattca tatcaataaa tttgaaaaaa gctgtgaaaa
    58021 aaactgggat tattttgtag aaaataaatc tataaatggt acatttctgc aaactagaaa
    58081 tttccttaat tatcatgaag aaggaaaatt cactgaccac tcattaataa tatataaagg
    58141 tgaagaaata ctagcagtcg tacctgcatg tgaaacttat gaaaatggtg aaagagtatt
    58201 tttttctcat ttaggaagca cttttggagg tattataata agaagtggtt ttaatgatat
    58261 taggcatgta aatgctatta caaataagtt agaaacatat ttacaggaac aaagtttcaa
    58321 taaagtatat ttaaaaaata catcaagaat attttgtaaa gaggatactt cattactaga
    58381 atattttcta tttaagaata attacatgtt ttatgatgaa ttaagtatgt ttttagaatt
    58441 aagtaagtat aaaaaagata tattagctaa ttttagatat agtaaaagaa gatattttaa
    58501 atattctttg aagaataatc ttacctttaa aagattgtat agtgatgagg atatagtttg
    58561 tttttttaat ttactttcta attgtttaga tagtaaagga gctaaagtaa tacattcagt
    58621 agaagaactt ttggagttta aaaataatag attaaaagat attgtagaat tttatgcagt
    58681 atatattgat gacattatga tttgtgggag catggtattt aactttcaag aaagagttta
    58741 tcatactcaa tatctagcat caaattcgac atataataaa tattatccaa tgatattttt
    58801 atactataat ctaataaaag atgctttaga gagaaatttt aagtatcttt cttttggaat
    58861 tagcactcta aatagaggca aatacttaga cgagggttta gctacattta aagaaggttt
    58921 tggttcaagt gcaatttgca acagaacata ctataaaaat ataaataagt gataatcatg
    58981 ttttatagag tttttagagt atagtaaata tctagtactc taatattaca taaaaataat
    59041 tagtttataa agaaggtttg tatatgaaaa gattaatgat tttaggctca attaagtatt
    59101 ttgagaatat tgttatgtca gctaaaaaag agggaatata cacaattgtt tgtgataata
    59161 gaattgatac tcctgcgaaa aagatttgtg atgaagcaat agatgttgat gtatttgatt
    59221 ttgataaaat aaaaagtata gctatagata aaaagataga tggaatttta actggattta
    59281 cagattcttt aatgaaacca tatgtttata ttgcaaatga actaaattta ccatgtgtga
    59341 tttcttgcaa tcaacttgaa tctgtaacta ataaggctat tatgaaaaaa tattttaaaa
    59401 ataatgcaat acctacaact gattattgta ttattgaaag tctaaaagat ttagaaaaaa
    59461 tacaaggatt aaattttcca cttgtaataa aacctgttga tggttatggt tcaagaggtg
    59521 tatattttgt atcaaatata gaagaattaa tagaaaaatt tgaatattct aaggaagcct
    59581 cacaagatgg acaagtaata attgaggaat tttatgaaag tgaggaaata caaggactag
    59641 cttgggttca taatggagag tcacatgtat tttatattgg agatagagag cttgtaaata
    59701 tacatacagg aagagctgga aaaccagata gattacttta tccatcaaag tattgttttc
    59761 aatatgagga agaaataaaa tctatatatc aaaatataac acaggctttt gatataaaaa
    59821 atggacctct ttatgtacaa atgttagtgg gagcatatgg aataaaggta tctgaagtaa
    59881 tgccaagatt acctggaggt tgtgactata tggctatttc tcaaataaca gatttagaca
    59941 taggaagttt atttgcaaac tttagtgtga ataatgaagt aaattttgat gaaataaaga
    60001 aatataatat gcatttatca aaatgtgtat atgcattacc aatatacata agacctggaa
    60061 caataaaaaa gataaataat atagataaaa tagaaaactt agattatgtc actcaatttc
    60121 tattattagt aaaagaagga gatacaatag gattatatga ggatgagaga caggattgtg
    60181 gaagagttta tggaatagca aataatatat ttgatgcaaa tataaagaag aaatgtattc
    60241 atgaaatgat agaaatattt gatgaaaata atgaaaatat gatagaaaat ttttaaataa
    60301 gggagtatga tagatatgaa gctaataact catgaacaaa ttatgtcatt aaacatttct
    60361 cagaaacaag gatatgaatg gataaaagaa tcttttttaa caaaaaaaga ttgtcaatta
    60421 ccaccaaaaa taagcatggt agaagaaggt catgtatttt acaatgtaat gccgtcctta
    60481 aatcataacc atatgattgg tggaataaaa atggttacaa gattgccagg gagaaatcca
    60541 agtttaaatt cagatatatt tctatacaat atgaaagatg ctaaattaaa ggctattttg
    60601 gatggtaatt atattacagc ttttagaact gctgctgttg ctggtttggc tattgatact
    60661 tttgctaaaa aagattttaa tactatttct attatgggtt taggtgtaac tgcttctgct
    60721 ataatgaata tgctttcgta tactcttaaa gataaaaata taaatataaa gttacttaaa
    60781 tataaagacc aagcagagtc atttataaaa aagtatgaaa aaaatgaaca atttagtttt
    60841 gaaatatgtg atacacatga agaacttatt gtagattcag atgtagttat ttctactgtg
    60901 acatatacag atgaatattt atcagaattt aaatggttta aaaaaggtgc tttattatta
    60961 cctgtacatg ttatgggatt tggaaatgtt gattatctat ttgataaagt atatttagat
    61021 gattataatc atttgaaaca tctagaaaat atagatagat ataaatattc ttgtgaattt
    61081 tataatgttt taagtaaaca agctaaagga agagaaaatg atgatgagag aataatatct
    61141 tacaatatag gtttgtcaat acatgatata ttatttgcaa acaaaattta cgaattgtgt
    61201 gaaacactaa attgtgaaga gatagaatta aaagaaccaa aagaaaaata ttggatatag
    61261 tttatttcat tcatatttac aataatttat tgtaaatatg aatatgaagt aatatattgt
    61321 tagaaaatta gcatgaatag gatagataaa tttatggata aatataattt aattaagttc
    61381 ttatctattg gaaacgaata tataggaaat ttagttgcat tagaatcaaa taaagcaata
    61441 ccttttgata taaagagagt ttattacata tatgatgtac caaacaatgt aaagagagga
    61501 tttcatgcac acaaaaactt ggaacaagta ctagtttgcc taaatggaag tgtgaagata
    61561 aaatgttatg atggtgtaaa agagagtata tttaatttac ataaaaatga tgttggattg
    61621 tatataggaa cgggagtgtg gcatgagatg tttgactttg atgaaaaaac tgttttaatt
    61681 tctatagcat ctgaacatta caatgaagaa gattatatta gagactataa agaattttta
    61741 aagtatttaa ataatatgta gttattatac agttagtgat gtattttctt aattaaataa
    61801 atttaagata gccatataga gaataaatag attaggatgt gaatttattg ttggttaaat
    61861 ttaataattt attaaaacag ttccaaactc ataaagaaga atatgaacag aagattaagg
    61921 atgtaatgga aagtggatat tatatccttg gaaatgaagt aaggcatttt gaagaagaat
    61981 ttgctagcta tgtaggtagc aaatattgtg ttggtttagc cagtggttta gatgcactta
    62041 taattgcttt tagagcatta ggaataagtg aaggtgatga agtaatcgtg caatcaaata
    62101 catatattgc tagtgttatg ggaatcagca taaatggagc aacaccagta tttgtagaac
    62161 caaatgaata ttataatata gattcatcta agatagaaga aaagataaat agcaaaacaa
    62221 aagctatatt agtagttcat ttatatggtc aagcaagtaa aatgaaagat atacaagaat
    62281 tatgtgataa gtataacttg tatttaattg aagattgtgc tcaatctcat ggagctaaat
    62341 atgatggaaa aatgacagga acatttggtg atataggttg ttttagtttt tatccaagta
    62401 aaaatttggg tgcatttgga gatgcaggag ctatagtaac tagtaatgag gaaatagctg
    62461 ataaagtgaa aattcttaga aactatggtt cagaaaagcg atattataat aaatttgtcg
    62521 gatataattc aagattggac gaaattcagg caggtttatt aagagtaaaa cttaaatatc
    62581 taaatgaaat tgaaattgaa aaatcaaaaa tcgtagatag atatcttaat gaaattaata
    62641 atcccaaagt catacttcca aagatagaaa ataattgtac tcatgtatgg cacttatttg
    62701 ttttacaagt tgaaaaacga gatacattta tggaatactt aaagaaaaat ggtatagagt
    62761 ctataataca ttatccgata ccaccacatc tatctgaagc atatgcttat ttgggatata
    62821 aaaaggggag ttttaatata gctgagaact attcagataa agttgttagt atacctttat
    62881 ttgtcggtat ggaagatgaa gaagttagtt atataattaa aattataaat gaatattaaa
    62941 ctaatcataa taatatcaaa aataaatttt ctatatatca tttatttaaa tttcgttaat
    63001 tgacattttt aaatattgat atattgtaca aataaaa
//
LOCUS       DAMKPG010000008        62600 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMKPG010000008 DAMKPG010000000
VERSION     DAMKPG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.009"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..990
                     /gene="dprA"
                     /locus_tag="PC621_000598"
     CDS             <1..990
                     /gene="dprA"
                     /locus_tag="PC621_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF4107784.1"
                     /translation="SEKEIYNLKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYIC
                     KYDKEYPENLKNIYNAPKLLFYKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQL
                     SQYGVNIVSGLAIGIDAYSHIGCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGG
                     LLLSEYNINSTVAPYHFSNRNRIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFA
                     IPGNINSQMSRGCHKIIKEGAKLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSK
                     QIIEAIKREGNLHIDSICDYTGIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            1023..3110
                     /gene="topA"
                     /locus_tag="PC621_000599"
     CDS             1023..3110
                     /gene="topA"
                     /locus_tag="PC621_000599"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF4107785.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            3275..4060
                     /gene="codY"
                     /locus_tag="PC621_000600"
     CDS             3275..4060
                     /gene="codY"
                     /locus_tag="PC621_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF4107786.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            4167..5432
                     /locus_tag="PC621_000601"
     CDS             4167..5432
                     /locus_tag="PC621_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF4107787.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            5463..6233
                     /locus_tag="PC621_000602"
     CDS             5463..6233
                     /locus_tag="PC621_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4107788.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            6342..6788
                     /locus_tag="PC621_000603"
     CDS             6342..6788
                     /locus_tag="PC621_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF4107789.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            6806..7999
                     /gene="nifS"
                     /locus_tag="PC621_000604"
     CDS             6806..7999
                     /gene="nifS"
                     /locus_tag="PC621_000604"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF4107790.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            8053..8493
                     /gene="nifU"
                     /locus_tag="PC621_000605"
     CDS             8053..8493
                     /gene="nifU"
                     /locus_tag="PC621_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF4107791.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            8782..9861
                     /gene="mnmA"
                     /locus_tag="PC621_000606"
     CDS             8782..9861
                     /gene="mnmA"
                     /locus_tag="PC621_000606"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF4107792.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    10004..10242
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10310..12949
                     /gene="alaS"
                     /locus_tag="PC621_000607"
     CDS             10310..12949
                     /gene="alaS"
                     /locus_tag="PC621_000607"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF4107793.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            12974..13231
                     /locus_tag="PC621_000608"
     CDS             12974..13231
                     /locus_tag="PC621_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF4107794.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            13241..15412
                     /locus_tag="PC621_000609"
     CDS             13241..15412
                     /locus_tag="PC621_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107795.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            15618..16043
                     /gene="ruvX"
                     /locus_tag="PC621_000610"
     CDS             15618..16043
                     /gene="ruvX"
                     /locus_tag="PC621_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF4107796.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            16121..16408
                     /locus_tag="PC621_000611"
     CDS             16121..16408
                     /locus_tag="PC621_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF4107797.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            16690..17148
                     /locus_tag="PC621_000612"
     CDS             16690..17148
                     /locus_tag="PC621_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF4107798.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(17265..18002)
                     /locus_tag="PC621_000613"
     CDS             complement(17265..18002)
                     /locus_tag="PC621_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF4107799.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            18219..19895
                     /locus_tag="PC621_000614"
     CDS             18219..19895
                     /locus_tag="PC621_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF4107800.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            20101..20301
                     /locus_tag="PC621_000615"
     CDS             20101..20301
                     /locus_tag="PC621_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF4107801.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            20355..21518
                     /locus_tag="PC621_000616"
     CDS             20355..21518
                     /locus_tag="PC621_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF4107802.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(21729..22151)
                     /locus_tag="PC621_000617"
     CDS             complement(21729..22151)
                     /locus_tag="PC621_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF4107803.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(22172..23008)
                     /locus_tag="PC621_000618"
     CDS             complement(22172..23008)
                     /locus_tag="PC621_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4107804.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            23306..23902
                     /locus_tag="PC621_000619"
     CDS             23306..23902
                     /locus_tag="PC621_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF4107805.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            23932..24651
                     /locus_tag="PC621_000620"
     CDS             23932..24651
                     /locus_tag="PC621_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF4107806.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            24653..25192
                     /gene="scpB"
                     /locus_tag="PC621_000621"
     CDS             24653..25192
                     /gene="scpB"
                     /locus_tag="PC621_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF4107807.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            25354..25983
                     /locus_tag="PC621_000622"
     CDS             25354..25983
                     /locus_tag="PC621_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF4107808.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            25937..26365
                     /gene="ytfJ"
                     /locus_tag="PC621_000623"
     CDS             25937..26365
                     /gene="ytfJ"
                     /locus_tag="PC621_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF4107809.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            26613..27053
                     /locus_tag="PC621_000624"
     CDS             26613..27053
                     /locus_tag="PC621_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4107810.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            27069..28520
                     /locus_tag="PC621_000625"
     CDS             27069..28520
                     /locus_tag="PC621_000625"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF4107811.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(28611..29279)
                     /locus_tag="PC621_000626"
     CDS             complement(28611..29279)
                     /locus_tag="PC621_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF4107812.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            29548..29688
                     /locus_tag="PC621_000627"
     CDS             29548..29688
                     /locus_tag="PC621_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107813.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(29863..30183)
                     /locus_tag="PC621_000628"
     CDS             complement(29863..30183)
                     /locus_tag="PC621_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF4107814.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(30303..31040)
                     /locus_tag="PC621_000629"
     CDS             complement(30303..31040)
                     /locus_tag="PC621_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF4107815.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            31255..33078
                     /gene="acd"
                     /locus_tag="PC621_000630"
     CDS             31255..33078
                     /gene="acd"
                     /locus_tag="PC621_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF4107816.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            33293..37591
                     /locus_tag="PC621_000631"
     CDS             33293..37591
                     /locus_tag="PC621_000631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF4107817.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            37795..38256
                     /locus_tag="PC621_000632"
     CDS             37795..38256
                     /locus_tag="PC621_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF4107818.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            38286..39440
                     /gene="nusA"
                     /locus_tag="PC621_000633"
     CDS             38286..39440
                     /gene="nusA"
                     /locus_tag="PC621_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF4107819.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            39447..39728
                     /locus_tag="PC621_000634"
     CDS             39447..39728
                     /locus_tag="PC621_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF4107820.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            39718..40029
                     /locus_tag="PC621_000635"
     CDS             39718..40029
                     /locus_tag="PC621_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF4107821.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            40051..41991
                     /gene="infB"
                     /locus_tag="PC621_000636"
     CDS             40051..41991
                     /gene="infB"
                     /locus_tag="PC621_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF4107822.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            42034..42414
                     /gene="rbfA"
                     /locus_tag="PC621_000637"
     CDS             42034..42414
                     /gene="rbfA"
                     /locus_tag="PC621_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF4107823.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            42395..43378
                     /locus_tag="PC621_000638"
     CDS             42395..43378
                     /locus_tag="PC621_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDF4107824.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            43391..44296
                     /gene="truB"
                     /locus_tag="PC621_000639"
     CDS             43391..44296
                     /gene="truB"
                     /locus_tag="PC621_000639"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF4107825.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            44427..45356
                     /locus_tag="PC621_000640"
     CDS             44427..45356
                     /locus_tag="PC621_000640"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF4107826.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            45460..45717
                     /gene="rpsO"
                     /locus_tag="PC621_000641"
     CDS             45460..45717
                     /gene="rpsO"
                     /locus_tag="PC621_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF4107827.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            45957..46802
                     /locus_tag="PC621_000642"
     CDS             45957..46802
                     /locus_tag="PC621_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF4107828.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            46927..49038
                     /gene="pnp"
                     /locus_tag="PC621_000643"
     CDS             46927..49038
                     /gene="pnp"
                     /locus_tag="PC621_000643"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF4107829.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            49304..50047
                     /locus_tag="PC621_000644"
     CDS             49304..50047
                     /locus_tag="PC621_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4107830.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            50205..51452
                     /locus_tag="PC621_000645"
     CDS             50205..51452
                     /locus_tag="PC621_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF4107831.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            51487..51732
                     /locus_tag="PC621_000646"
     CDS             51487..51732
                     /locus_tag="PC621_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF4107832.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            51742..52965
                     /gene="dapG"
                     /locus_tag="PC621_000647"
     CDS             51742..52965
                     /gene="dapG"
                     /locus_tag="PC621_000647"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF4107833.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            53078..53812
                     /locus_tag="PC621_000648"
     CDS             53078..53812
                     /locus_tag="PC621_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF4107834.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            53935..56346
                     /locus_tag="PC621_000649"
     CDS             53935..56346
                     /locus_tag="PC621_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF4107835.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            56349..57407
                     /gene="mnmH"
                     /locus_tag="PC621_000650"
     CDS             56349..57407
                     /gene="mnmH"
                     /locus_tag="PC621_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF4107836.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            57401..58735
                     /gene="rimO"
                     /locus_tag="PC621_000651"
     CDS             57401..58735
                     /gene="rimO"
                     /locus_tag="PC621_000651"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF4107837.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            58722..59264
                     /gene="pgsA"
                     /locus_tag="PC621_000652"
     CDS             58722..59264
                     /gene="pgsA"
                     /locus_tag="PC621_000652"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF4107838.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            59552..60598
                     /gene="recA"
                     /locus_tag="PC621_000653"
     CDS             59552..60598
                     /gene="recA"
                     /locus_tag="PC621_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF4107839.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            60774..62315
                     /gene="rny"
                     /locus_tag="PC621_000654"
     CDS             60774..62315
                     /gene="rny"
                     /locus_tag="PC621_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF4107840.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        25333 a         6588 c        11616 g        19063 t
ORIGIN      
        1 tctgaaaaag aaatatataa tttaaaaaat ataagcttga atattagaaa aaatatagta
       61 aaatatagag gttacgctta tttagaaaat ataaaagaac tactttacaa aaaagcgatt
      121 aaatatattt gtaaatatga taaagaatat cctgaaaatt taaagaatat atacaacgct
      181 ccaaagttat tattctacaa aggtgacata ggtttagtta ataataattt taatatagct
      241 atagtaggtt caagaaaacc tacagcctat ggaattaatt gtgcaaaaac aataagttgt
      301 caactatccc aatatggagt taatatagta agtggattag ctataggaat agatgcatac
      361 tcgcacatag gttgtatgag tggaaaatct aaaactatag cagtacttgg ttcaggagta
      421 gacaatcctc ttccaaaaca aaatttacat ttatcaaata aaatattaga aaatggggga
      481 ttattattat cagagtataa tattaactcg acagtagccc cgtaccattt ttctaataga
      541 aatagaatta tcagtggttt aagtgatgga gtagttgttg tagaagcagc aataaaaagt
      601 ggtgcactaa tcacagtaga ttttgctctt gaacatggaa aaaatgtttt tgcaatacct
      661 ggtaatataa attctcaaat gagtagaggt tgtcataaaa ttataaaaga aggtgcaaaa
      721 ttaattgaaa atatagatga tattttaaat gaatataaca tatttaatat tattgataaa
      781 aaaataaatc aaaaatatga taatataagt ctaaatgcca agtctaagca aataattgaa
      841 gctataaaaa gagaaggaaa cttgcatata gatagtattt gtgattatac tggtatagaa
      901 ataaagtatg taaattcaat aatcaatgaa ttagtactaa atgagttagt agtagaaatg
      961 aataataaaa cgtacagttt aaatgtataa attaagatta ggtagtgggg gtgtatggtt
     1021 ttatggccaa aacattagtc atcgtagaat cgcctgcaaa agctaaaact atagaaaaat
     1081 ttttagggaa aagtcattac acagttaaag catcagttgg tcatgtcaga gatttaccta
     1141 aaagtaaatt aggtgtagat atagaaaata attttgaacc tcaatatata aatataagag
     1201 gaaaaggcga tgtaataaaa gaattaaaaa aagaagctaa aaaatctaaa caagtatatc
     1261 tagcaactga ccctgataga gaaggtgaag ctatatcatg gcatttagct catatactaa
     1321 atttagatga atctgaggat tgtagaatag agtttaatga gataactaag gatgcaatta
     1381 aaaaagcaat taaacatcct agaaatattg atataaattt ggttgatgca caacaggcaa
     1441 gaagagtgct agacagactt ttaggttatc aaataagtcc tatattatgg caaaaggtta
     1501 gaaaaggtct tagtgcaggt agagtgcaat ctgttacaac aaagttaatt tgtgatagag
     1561 aaaaagaaat aaaggcattt attccaaaag aatactggac aatagatgtt gaggctaaga
     1621 cagtaaaaga caaagatata actttgaaat tttatggtaa aaatgatgaa aagattgaat
     1681 tagaaaatga agaagctgta aatgaaatac taaaagaaat ggaaggtaaa gatttaaagg
     1741 ttgagaagat agaatctaaa tcaagacgta aatcagctcc aaaaccattt acaacaagta
     1801 tgcttcaaca agaaggagca aataagttat tttttactac taaaaaaaca atgatgatag
     1861 cacaagaatt atatgaaggt atcgacatag aaggtgaagg tactgttggt cttatatcat
     1921 atataagaac tgactctaaa agaatatctg atgaagcaaa agaaaaagct aagggttata
     1981 tcgtaaacaa tttaggagaa aactactata aaaacactgg tgataaaaaa gaaaaaaagg
     2041 atactaaaaa ggttcaagat gcccatgagg caataagacc aacctcagtt gatagaactc
     2101 cagagagtat aaaagcctca ttgagcaaag accagtacaa gttatacaat ttaatttgga
     2161 gaagatttgt agctagtcaa atggaagatt cagtgtttga tatactaaat gtagaatgta
     2221 aaataggcga tattgtattt aaggcaactg gttcaaaaat gaaatttgat ggttatacca
     2281 aggtatataa tttttcagaa agagaagaca aaattttacc aagtatagaa aaaggagata
     2341 tattaaatat agaaaatata ttgccagacc aacattttac acaaccacca gcaagatata
     2401 cagaagctag tttagtaaaa acactagaag aactaggtat aggtagacca agtacgtatg
     2461 ctccaacaat agctactata ctgaatagag agtatgttga aaagcaagga acaagtctat
     2521 gtccaacaga acttggaata atagttactg atattttgga aaataatttc cataaattta
     2581 tagatgtaga ttttactgct gatatggaaa gtaaacttga tgttatagaa gaaggcaata
     2641 cagattggaa aaagattgta tcagaatcat atgcaccact taaagaggct attgaagaag
     2701 ctcttgaaaa tatagaaaaa gtaaatatgg atgaagaaac tgatgagatt tgcgagaact
     2761 gtgggtctaa tatggttatc aaatatggta gatttggaaa gtttatggct tgtaaaaact
     2821 atcccgattg taaaacaact agaccaatta tgaataaaat tggtgttaaa tgtccaaaat
     2881 gtgaagaagg agatattata cttagaaaat ctaaaagagg taaagctttt tatgggtgtt
     2941 ctaattatcc aaactgtgac tttgtagctt ggaataagcc aacaggagag ttttgtgaga
     3001 agtgtggttc atatatggtt gaaaaagtga ctaaatctga aacaaaaaca gtgtgttcta
     3061 ataaagaatg taataaagag acaaaagagc ataatgacgg aaaagagtga aaattgaaaa
     3121 aaatcacata attctcttga aaaaggttcc aatctatgat aatctttcaa gtgtaaacaa
     3181 ctaatagaaa aataaatttt cattaaatgt gaaaataata ttagaaaata taaaaggtta
     3241 ttaaaaggcc taaatttcat gaggagatga ttaaatggca agtgaagtgt tacaaaaaac
     3301 aaggaaaata aataaaacat tacaaacaag tggtggaagc agtgtctctt ttgatttact
     3361 ggccggagca ttgggcgacg ttttaagttc taatgtttat gtagtaagtg caaaaggtaa
     3421 agtactaggt cttcatttaa atgatgttca agacagttca gttatagaag atgagtatac
     3481 taagcaaaag aaattttcag atgaatatac tcaaaatgtg ttaaaaattg atgaaacatt
     3541 agaaaattta aatggtgaga agatattaga aatctttcct gaagaacatg gaagattaca
     3601 aaaatatact acagtagttc caatattagg aagcggtcaa agattaggaa cattggtact
     3661 ttcaagatat tcaaattcat tcaatgatga tgatttagta atagctgaat acagtgcaac
     3721 tgttgttggt cttgaaatat taagagcaat aggtgaagaa ttagaagaag aaatgagaaa
     3781 gaaagctgta gttcaaatgg caataggcac tctgtcctac tccgagcttg aagcagttga
     3841 acatattttt gctgaattgg atggaaaaga aggtctactt gtagcaagta agatagctga
     3901 tagagttggt ataactaggt ctgtaatagt aaatgcactt agaaaatttg agagtgcagg
     3961 tgtgatagaa tcaagatcat taggtatgaa aggtactcat ataagaatac ttaatgacaa
     4021 acttacagat gaattaaaaa aattaaaaaa caatcaataa tctatatttt ataggtttag
     4081 attagaaaag gtatctattt caaatagata ccttttttag acgaattatt aaggtaatga
     4141 taagtatatt aaaaaggagt gagaaaatgc ctttagcaga taaaataaga ccaaaaacaa
     4201 tgaatgatgt aataggtcaa agtcatataa taggtaatgg aaaaatattg tctaagatat
     4261 tacaatctaa ttttttacca aatatgatat tttttggtcc tcctggtgtt ggtaaaacta
     4321 cagtagcgga aataatagca gaaagaagca ataaaagctt ctataaaata aatgctacta
     4381 attcatcttt agaagatata aaaaaggtga tatcagagct tggaagtata aataatgtaa
     4441 atggagtcct attatatata gatgaaatac aaagttttaa taaaaaacag caacaatcaa
     4501 tattagaatt tatggaaaat gggagtataa ctttgattgc tagcactaca gaaaatccat
     4561 atcattatgt atataaagct cttttaagta ggtctactgt atttgaattt aaacctttag
     4621 aaaaacttga tatagaaaaa ggactgaaaa gagctgttga agtattaaat gaagatagct
     4681 atatggatat tgagtgcaat aataatgcta ttgaagatat agcaattctt tctgatggag
     4741 atatgagaag agccttaaat attttggaag tggtagtcta ttcaactaaa ccaaataagg
     4801 ataatataac ttatatagat tcagatgtaa taagggcatc tacatttaat aaaattataa
     4861 actatgacaa aaatggtgat agtcattatg atattttaag tgcatttcaa aaatctataa
     4921 gaggaagtga cccacaggca tctatacact atctagcaag acttataaaa ggtggggatt
     4981 tgataagtat atgtaggaga cttttagtaa tagctagtga agatataggt ttagcttatc
     5041 caaatgctat tgtaattgta aaggcatgtg tagactcagc tatgcaactt ggatttccag
     5101 aagcgaaaat accattagca gaagctacta ttcttttggc tacatctcca aagtctaatt
     5161 ctgcatgtat agcaataatg aaagctatgg atgaactgga taaagagttt gttaaggata
     5221 taccaaatag tataaaggat gctcactatt ctggttctaa agaaataggt aggggttgtg
     5281 gatacaaata tcctcataat tttaaaaatc attatgtaaa gcaacagtat ttaccagata
     5341 gcataaaaga tagtatttat tacataccac aagaaaataa aactgagaaa aatataaaaa
     5401 aatatttaga atatctaaat agtgattttt agactgaatt ttatgtatcg ggggtagata
     5461 ttatgattat aaaacttaac tcaacttatc acagtagagt tatgaaatac ttaaaaaaag
     5521 aacctgagta caatttgttt ataatagggg atattgaaag atatggatat ggaaataatt
     5581 ttttaaatat atgggcagac gtgggtgaac atggtgaaat aaaggcaata ttacttaaat
     5641 attttgaatt tatgatgttt tattctgatg gagagtatga tgttgaaggt ttttataatc
     5701 tacttcgtaa tactaattac gaagaaatat ctgggaaaat atgtgctgta gatactttag
     5761 caaagagatt aggacttaat aatttaaagg tagttgattt ttgcaaactt caaactaaaa
     5821 agtttttgat agataataat tgtaatgcaa aggttaagag aattaggctt ggaaatctaa
     5881 aaaagacagt gaaactttat gacttaatag atgaatttca cagtacaact ttagagaatt
     5941 taaaaaatgg actaaggaca ggtagaggat attgtataga gattaacaaa caggtggtgt
     6001 caatggcaaa gagtacttca gaaaatagga ctcatgcaat gataattgga gtaggaacac
     6061 atcctaaata tagagctaaa ggacttgcta caaaatgcct aattaaacta tgttcagagt
     6121 tactgagaga aaacaaaatt ccttgtttgt tttatgataa tgaagaggct ggaaagatat
     6181 ataaaaaact aggatttgaa aacattggaa aatggggaat ttattcaaaa taaaaaatta
     6241 aaattaatct tgacaaaatt agtagggtat aataaaatta tcttgaaagt aattcctact
     6301 aaaacagtat gaattagatt tctatataga ggtgataata tatgaagttg tctactaaag
     6361 gtagatatgg actaaaagca atgtttgaat tagcattaaa tcaagacaat ggaccagtat
     6421 cattaaaatt tattgctaag aagcaaaaaa tatcagatca gtacttagaa caaatatttt
     6481 catctttaaa aaaatcgggt ctagtaaaaa gtgtgagagg tgctcaagga ggttatttgc
     6541 tttcaaagaa tgctgaagac ataacagtag gagatatact tgtagtatta gaaggaccag
     6601 ttgcattatc tgactgtgta ctagatgaag atgtatgtga aaactcaaac atgtgtgtaa
     6661 caaaaatagt ttgggaaaag atgaaaaagg gtatagaaga tgttatagat tcaattactc
     6721 ttaaagatat gataaatgac tataacaaaa ataaattaga aaatgatata acaaatatta
     6781 aaaaatagat ttggagatga atgtaatgga aaaaagaaga ttgtatatgg attattctgc
     6841 aacaacacct attaaaaaag aagtattgga tgcaatgatg ccatacctaa cagattattt
     6901 tggtaatgca tctagttttc acacattcgg aagagaagca aaagatgctt tggataaagc
     6961 aagagaacaa gtagcagccc ttataaatgc tgaacctagt gaaatatatt ttacagcagg
     7021 tggttcagaa agtgataact ggacactaga gggtgtagct tatgctaata aaaacaaagg
     7081 taatcatata ataacttcaa aaatagagca ccatgcaata cttcatacat gtgaatactt
     7141 agcaaaacat catggatttg aaataactta tttagatgtt gatagcgaag gaagagtaga
     7201 cttaagacaa ctagaagatt caataaaaga tactactata cttataagca taatgtttgc
     7261 aaataatgaa ataggaacta tacagcctat taaagaaata tccgaaatag ctaaaaaaca
     7321 taagatatta ttccacactg atgcagtaca agctacaggt aatattccag tagatgttaa
     7381 agaattagga atagatttga tgagtatgtc ttcacataaa atatatggac caaagggtgt
     7441 aggagcttta tacataagaa aaggtgtaag acttcataac tttgtccatg gtggagcaca
     7501 agaaaaaagc aaaagagctg gtacagaaaa tatacctgca atagttggat atggaaaagc
     7561 agcagaatta gctaaggaaa atatgcaaaa tcatgtagaa actttaactc gtcttagaaa
     7621 taagttaata gatggtgtgt tagaaagaat accttacaca agagtaaatg gtagcttaga
     7681 aaatagattg ccaggaaatg caaactttgc attccaattt atagaaggtg aaggaatact
     7741 tttattatta gatatgttag gaattgctgg ttcaagtggt tctgcatgta cttctggttc
     7801 attagaccct tcacatgtac ttcttgcaat aggtttacca catgaaatag cacatggttc
     7861 attaagactt acagttggag attttacaac agatgatgat atagattata tactagagaa
     7921 cttaccaaaa gtaatagaaa gacttagaag catgtcacca ctttatgatg atgcgaagaa
     7981 acaaggttta gtaaaataga cagaaagata aagcataaaa aatagacata aattgagtag
     8041 gaggaaaaga taatgcaata tagtgataaa gttatggaac attttatgaa tccaagaaat
     8101 atgggagaaa ttgataatgc aagtggtgta ggtgaagttg gaaaccctac atgtggagat
     8161 ataatgaaga tatttttaga catagatgga gatgtaataa aggatgttaa gtttaaaaca
     8221 tttggctgtg gttcagctat agcaagttct tctatggcaa ctgaaatgat aaaaggaaaa
     8281 actataaaag atgcattaga acttacaaat aaagctgttg cagaagcttt ggatggttta
     8341 ccaccagtaa agatgcattg ttcagtttta gctgaacaag ctgtaaaagc agctcttata
     8401 gattatgctc aaaagaacaa tatccatata ccagaattgg atggatatgt tatagatgat
     8461 gctcatgacc atgatgttga agaagaagaa taaaaataag tgattgtgtt atgagaagtt
     8521 tacaatgatt aaaaagaagt actaatgata taattaggac ataaaaaata gctgttttta
     8581 aaatggctat tttttattgc ctaaattaag tattttaact tgatttagag gttaattagt
     8641 taacaaaata atccataaga ttcaaataat aaagatgatt atttttttag aaaatagaac
     8701 aagctatatt ttaaataagt acaatacttg ctctatatgg tataataata agtaatgcaa
     8761 aagatgaggt gaaatttgtt tatgaataaa aaagtaatga taggtatgag tggaggagtt
     8821 gatagctctg tagctgcata tcttttaaaa caacaaggat atgatgtcat tggtgttact
     8881 atgaagttgt ggcaagatga tgatgttgtt gaaattgaag gtggttgttg ctctttatct
     8941 gctgttgaag atgctagaag ggtagcaaat aaaataggta taccatttta tgttttaaat
     9001 tttagagaag tgtttaaaga aaaagtaata gattatttta tagatgaata tttggaagga
     9061 aaaactccta atccctgtat agcttgtaat aaacatataa aatttgatga tttttataag
     9121 aaggctagac aaataggttg tgattatgta gctacaggtc actatgcaaa gatagaaaag
     9181 gatgaaagta caggaagata tttgctaaaa aaatctgtta cagataaaaa agaccaaact
     9241 tatgcacttt ataatcttac acaagagcaa ctagagcata ctttattgcc aataggtgac
     9301 tatgaaaaag atagagtaag agaaattgct aaggaaatgg ggatggcagt acacaataag
     9361 cctgatagtc aagaaatttg ttttgttaaa gataatgact atgcaaatta tgtaaaaaaa
     9421 cattctaaaa agcgtattga agaaggtttt tttgtagata ctaaaggcaa tatacttgga
     9481 aaacataagg gtatattata ttatactata ggccaaagaa aagggcttgg aattactttt
     9541 ggaaaaccaa tgtttgtaat agacataaat ccaataaata ataccatagt tcttggagac
     9601 aatgaagatt tatttaaaaa ggaacttatt gctaaagatg ttaactttat atcaatagat
     9661 acgttagaag aacccttaag agttcaagcc aaaataagat attcagcaaa accatcacca
     9721 gctactattc atagagtagg agaagatact attaaaatag tttttgatga agctcaaaga
     9781 gctattacta aaggtcaatc tgtagtaatg tatgatggag atattgtagt aggtggaggt
     9841 ataatcgaga aaagtttata aaaacatcta aaaaattaaa aaaataagca ataaagtata
     9901 ttaagagtaa ataaagttta taaaataaat ttattgtata ttaaaatata gtattgatta
     9961 attttgtaaa atttagtaat ataattagta tattaattaa aaaacaaata cagtgataag
    10021 gaatagtaaa atatttgatg tcttacagag agagggaaat gctggaagcc cttagaatat
    10081 gatatttgaa gcagcctttt agtagcaatc ttgaacaatt tatcttttat aaagaagatt
    10141 taagtagaga ttgacggtag ttacgttata gctaaggttt tccgaagata tcatactttg
    10201 tatgataatt agggtggtac cgcgattgtc ctcgccccta taaatttatt tatagggacg
    10261 aggtttttta tttaaacgaa aaaataaaat ttattaggag gaacatgata tggaaaagat
    10321 gggattaaat gaaataagaa gtaagttttt agaatttttt gagtcaaaag gtcattatgt
    10381 agcaaatagt tattctttag taccaaacaa tgataagagt ttgttactta taaactcagg
    10441 tatggcacct ttgaaaaact acttttcagg tgtagaagta cctccaagtg tgagaatgtg
    10501 tacatctcaa aaatgtataa gaacaggaga tatagaaaat gtaggtataa ctgcaagaca
    10561 tgctacattc tttgaaatga tgggtaactt ctcatttgga gattacttta aaagagagtc
    10621 aatcaaatgg ggatgggaat ttgttacaga atggctaaat ataccagaag ataagatatg
    10681 ggtgactgta tatgaagaag atgatgattc ttatgatata tgggcaaaag aaatgaattt
    10741 cccagaagaa agaatggtta gacttggaaa agatgataat ttctgggaaa taggtacagg
    10801 tccttgtggt ccttgttcag aaatatattt tgatagagga gaagaatatg gatgtgacaa
    10861 tccagactgt aaaccaggct gtgattgtga taggtattta gaattttgga accatgtatt
    10921 tactcaattt gatagagatg aagaaggaaa ttatagtcta ttagaaaaca agaatataga
    10981 tacaggtatg ggtcttgaaa gaatgggatg tattatgcaa ggcgtagaca ctatctttga
    11041 agttgataca attaaatcta tactagaagc agttgagaag ttaacaggag taaaatatgg
    11101 agagaatcca aaaaatgata tatcaataag aataataact gaccacataa gagctgtaac
    11161 attcttagtt agtgatggag tgcttccatc aaatgaagga agaggatatg ttcttagaag
    11221 actccttaga cgtgctgctc gtcatggtaa gttattagga gtaaaagaat tatttttaca
    11281 gaaactaata gatgaagtta taaaggtaaa tgataaagca tatccagttt tagttgaaaa
    11341 agaaagttat attaaaaaag tagttggaat agaagaagaa aaattcaatg aaactataga
    11401 ccaaggaaca gaaatattga actcttatat agaagtatta aaaaatgaag gaaagactgt
    11461 cctaagtggt caagaagctt ttaaactata tgatacttat ggcttcccta tagacttaac
    11521 taaagaaata ttagaagaag aacatctatc tgtagatgaa gaagctttta atgaagaaat
    11581 ggaaaagcaa aaagaaagag ctagaaatgc tagaggaaat atggatggag aaagttggaa
    11641 ggaagaccct ctatcaaaat tagaatctac agttgacagt acatttaatg ggtatagtga
    11701 aatttatggt gaaggaacta ttgaagctat agttaaagat gatgagctag ttcaaagtgc
    11761 agaagaaggc gataaggtat ctatagtact agataacact actttctatc ctgaaggtgg
    11821 aggacaagtt ggagattgtg gattgataac taatgaaaac ttagttttag aagtattaaa
    11881 tactaaaaaa ggtgctaata atagtataaa acatataggt ataataaaat caggaagaat
    11941 aagtaatggg gacaaagtaa agacactcgt agatagagaa actagaatgt cagctgcaag
    12001 aaatcacagt gctacacatt tacttcataa ggcattaaga gaagtattag gagaacatgt
    12061 taatcaagct ggttctttag taactccaga aagacttaga tttgatatta ctcactttga
    12121 agcgatatca aatgaagaat taaaagtaat agaagaaaaa gtaaataatg ttattttatc
    12181 atctttagac ataaaatgtg atattatgaa tataaaggaa gctaaagaaa aaggagctac
    12241 agcactattt ggtgaaaaat atggtgatga ggtaagagta gtttctatgg gagattattc
    12301 tacagaactt tgtggaggaa ctcacttgac aaatacttct caagtaggaa tgttcaaaat
    12361 attatctgaa ggtggagtag cagcaggtgt aagaagaata gaagcaataa caggaaaagc
    12421 tgtttatgaa tacctaaaag aaagagatgg aattatctct gaagtttgtg taaacttaaa
    12481 atctaaagaa gataatttaa ttcaaagaat aagttcttta ttagaagaaa ataaaaactt
    12541 atctaaagaa ttgcatgata tgaaagctaa gatgagttta caatcagttg attctatttt
    12601 tgattctaaa gttgaagtaa atggagttaa tttgataact aataaatttg aaggtatgga
    12661 tatggataca ctaagagaaa cagcagataa tctaagagat aagcttggtt ctggtgtagt
    12721 agtacttgca aatgtagtag atgataaagt taactttgta gttactgcca ctaaagatgt
    12781 attagataag ggaatacatt caggtaatat tgttagagaa gttgcaaaaa tagctggagg
    12841 aaaaggtgga ggaagaccta atatggcaca agcaggagct agtgatgtat ctaaggttga
    12901 ccaagcttta agctatgcaa gtgaggttat taagacacaa gttaaataac attcactcaa
    12961 ggaggttttt agtttggaaa aagatttaga ctacactatg aaatttgaag gaataccaga
    13021 agataggatg agtgtaggag atactattga ttttgtatat aaagcattag ttgaaaaagg
    13081 atataaccct ataaatcaaa ttatagggta tctcctttct ggtgattcta gctatataac
    13141 tagtcataaa aatgcaagag ctataattaa aaaatttgaa agagatgaga tacttgaaga
    13201 agtaatcacg cactatctga atagaaagta ggtcaaataa atgagaaaaa agattatgtc
    13261 gttgtttgca atagtcatta tgatttttgt atcaactgct agctatggtt ttgctgaaga
    13321 aaaaggcaag gtcatattta ttgatatgaa taggaccaat cttggaagta tgatgaatat
    13381 tccaatcttg aaaaatgaga tagaaaagag aggctatgta gctctcatga atataagagg
    13441 ggatcaaggt actgatgata gaaggtctta tgcaagtatg ggtgcaggaa caagagctag
    13501 tgtaactact gaagactata tcaattttca aaaagtagac caagatactg ctaaagcttt
    13561 tgaagtcgca acaggtcaaa aagctaaaga aataaatgat ttaggcatta atagatctat
    13621 aaatgaaaat cttgagtatg gagaatatgg agcaaattta gggtcattag gaaaaacttt
    13681 atcagaaaat ggtttaaaga catctgtaat tggaaatgct gatataattg aaaataatca
    13741 attagttaaa aatagaaact tatgtttagt agctatggac caatatggaa ggattgattc
    13801 aggtaatatt gataatatta ataagaaaga ttctaaaatg ccatttggaa tatctacaga
    13861 ctacgataaa ttactagatg aaactaaaaa atcctataaa aatagtgatg ctatatttgt
    13921 agaacttgga gatacatata gattagatat gtatagatta aatctaaatg ataatactta
    13981 tggtacaatg aagaaaaata ttgtaaaaaa tataaataaa tatcttgaac aagtattttc
    14041 tatggttgga gaaaatgaca ctgtttatat agcaagtgca tttccaagtg acctagatta
    14101 taagaataag agaagacttt ctccaataat aaaatttagt ggagaaggga aaggtctact
    14161 ggaatctgcc acaacaagaa aggctggaat tgttgccaat ttagatgttg gagtagatat
    14221 actaggtaca tttggtcttg aaaataaaga gatggtaggt agaagttata ctttagtaaa
    14281 taaagaggac aatgttggct atctaaaatc actttatgaa aggatagtgt ctatatcttc
    14341 aataaggtca actattataa atggctttgt aggcgtaata tctgcttctt gggtaattgc
    14401 catgtttgcg attttcttta gaaattatat gccacataaa gagcgtgtat tcaaagtact
    14461 taaggagttt ataaaattag gcataataat gccactatca tttatgttag ctcctttgat
    14521 gaattttaaa actccaattt caatatcttt gggaatattt gcgactacgc taatattata
    14581 cttgttaggt agaaaattat ttaaggacaa tgatataaag cacatgggat tttttgcaac
    14641 acttactatt ttagtcatag ttatagattc tgtatttgga acatacttaa tgcaaaataa
    14701 cataatgagt tatgatgctg tcataggagc taggtactat ggtgtaggaa atgaatatga
    14761 aggtgtaact atagcaagtg cagtatttgg cttagcagta ttattacatt ataagaaggt
    14821 atctaaatta cttgttgtaa ttttttcgtt aataatactt ataactagtg catatccttc
    14881 aatgggtgca aatgttggtg gagctatatc agaatgtgta gcatatttgt tgtttataat
    14941 gcttatatat gacattaaaa tagattttaa gaaagctgta ttattagctg tttcagctgt
    15001 agttgtcgtt tttgcatttg cagctttaga tatactttca ggtagtgaat ctcatttagg
    15061 tatgtttgta cagagaattt tcctaaatgg accaggagag atattacaaa catttggaag
    15121 aaaaattcaa atgaatatgc aactagcaca gacaagtgta tgggtaaata ttttacttgt
    15181 aggaattgga gtaattgcag tacttatatt taaaccatct agacacttta gaaagataat
    15241 gaataatttt ccaatcttgt ttaaagggtt tattgcatct atggttggtt gtataattac
    15301 tcttttagtt aatgattctg gaatagttgc tgcatctaca gcatctatat atatacttat
    15361 accactaata ataataaata taaatatgat aatatttgaa aaaaacaact aaaaaatttt
    15421 ttactgttca tataggattt aaataaaaaa tgcaatatga ttattaatgt aaactggatg
    15481 cttacataaa aaatcatatt gcaattttta tgattgatta tgcaaaattc tatgaagcag
    15541 gaagcctgtg accttagtta tgagaggttc agaatatgta tttaatctat agaatataaa
    15601 tagataaagg aggaactatg ttagatggaa gaataatggg tcttgatgtt ggtgataaaa
    15661 ctattggagt tgctgtgagt gatttgatgg gattaactgc acaaggtgta aaaacaataa
    15721 aaagagttgg caagaaaaaa gatattgaag agttaaaagc aataataaaa gaaaaacaag
    15781 taaataaaat tgtatcagga ttaccaaaaa atatgaatgg aactttaggt ccacaaggag
    15841 aaaaagtaat caagttttgt gaattggtaa aagcagaaac aggtattgat gtagagtttt
    15901 gggatgagag gctttctaca gtagcggcag agaggtcttt attagaagca gatgttagta
    15961 gacaaaaaag aaaaaaggtt atagatatgt tggcagcagt aataattttg caaggatatt
    16021 tagattttaa aataaattca taaaagaact ataaaattga atacttatga tatataatat
    16081 aaataataaa aataaaaata aatttgaggt gaatgacatt atggaagaaa atataataaa
    16141 tttaatagac gaaaatggcg ttgaaagcca atttgaaata atattaacat tagaagcaga
    16201 aggaaaagag tatgcaatat taatgccttt agatgatgaa gaagctgaag aagctttaat
    16261 ctttagaatt gatgaggatg aagaaggtga aatattaata cctttggaaa gtgatgaaga
    16321 gtatgaaact gttgtagctg tgtatactgc tataatggaa gaagaaggtt taaattacga
    16381 cgaagatgaa tcaaatggac ttaactagat aagtaattaa gtttgtatat aaaaatcaga
    16441 tatttattat atttgcttta attaaacctt attaattgta aaagcattta tagtatatat
    16501 ctgttttttt gttttttatt aaaaatacca aaaaatttat gaattttggt gaataatata
    16561 tcttttatgg gtatatattt atattatgag aataaattaa atttatttaa atgtcttgac
    16621 aaaaaatgaa ttatcaatta caatattatt taataaagat tatcaattga aaatagtaag
    16681 ggtgatgtta tggcaaatac aatggattta ttaaaagaca agttaaaaga aactggtttt
    16741 aagattacgc cacaaagaag agcaatagtt gaaatattgc ttaaacatga ccattcacat
    16801 ctaagtagtg aggaaatcta tgatttagtt agggttgact gtcctgaaat aggcttagct
    16861 actgtatata gaacaatgca gctattggat gaaataggat taatatcaaa actaaactta
    16921 gacgatggat gtataagata tgagattagt ctacataaag aagactgtca taatcatcat
    16981 catcttattt gtaaaaattg tggaaagata atggaagcta aagaagacct tttagacaat
    17041 atagaaaaag agatacaatc attgtacaaa tttaaaatac ttgatcatga tgttaaattt
    17101 tatggattat gtgacgagtg taatggagta agcgattcag aagaataaac ttctattgct
    17161 taatgtcgat ggaagaatag tatagaactt ttatattcta tgctattctt ccatttttta
    17221 ttataaacca tctacaaaat gcaaatatac agttttcaag atgcttatag ttttaatatt
    17281 gaaaatataa ttaatcctat aagtacaaaa atgaatgatt tttctatgaa attactaaat
    17341 ggtcctccaa cagttaaaaa acctaaagaa attttctttt cgcttagcgg ataaaaaatt
    17401 ggtattcctt gctttgtaaa taaatctcct aaaaacatat gacttgcata accaatcgta
    17461 ccataaaaag ataaattatt aatattagtg attaattcta tctgttttag taaaaaatat
    17521 actattatag ttgcaaatat gctatgagaa aaacttctgt gctttaacca gggaaaaatt
    17581 gacaacatta gacaaaaaat tgtaaaatat atttcaactt ctatgatgta taagcatata
    17641 gctaatagta taaatattaa tgatagaaaa acttttctta gaaatgtgtg ggttagtttt
    17701 gcttcaataa ttataattgc aacaaatgta attattgcgc ttaaataaaa attatcattt
    17761 atatttattg aaataaagaa aataataata ttaacagcat atataaaaat tctatatatg
    17821 tatttggata cattgttttt tagaatgaaa tttgagacca tagaattaga tttatccaaa
    17881 tcgggtagta cagcaaaagt agctgaaact aaaatatcaa ctaaagatat aggaatttta
    17941 aatattaaag atgtttggat agctgttaag attcctattg cacaatgagt tttaccacgc
    18001 ataaatcctc ctaaattatc cgatattaaa tcatataata tttagaggat atcaaaataa
    18061 aataggtttg tcaaaataat acgttaaatg tattttaaat atagcttaaa atatgatacg
    18121 aattatggta caataagaat aactggtagc gagtgcgtga tttgatatta tttatgattg
    18181 tagaacgtgt taaaaaatta aatagacaga aggagatgat gcaattgttc aaaaagaaca
    18241 ccaacaagat aaaggtaatg gccttgggtg gacttaacga agttggaaaa aatatgactg
    18301 ttgttgagta taaagatgaa ataattgtta tagatgctgg acttagtttt ccagaggatg
    18361 aaatgttagg agtagatata gttataccag atataactta tttagtaaaa aatagggata
    18421 aaataaaggg tatatttatt acacatggac atgaagacca tataggagct cttccatata
    18481 tattaaagaa aataaatgta cctgtatatg gggcaagact tagtataggt cttatacaag
    18541 taaaacttaa agaacataaa atgaataatg ttaagttaaa tgttataggt cctagacaag
    18601 ttataaagct tgataatatg gaagtagagt ttttgaaaaa taaccatagt ataccagatg
    18661 cttactctat tgcaatacac acagaccaag gtataatata ccacacagga gattttaaga
    18721 ttgacttgac tcctattgat ggagatgtta tggatatgca tagaatctgt gagttgagca
    18781 aaaaaggagt tcttttaatg ctggcagata gtactaatgc agaaaaacca ggatttacta
    18841 tgtctgaaaa gactgttggg gtaggtcttg acgaattatt tgcaaaagga aatggcagaa
    18901 gaattatagt tgctaccttt gcatccaaca tacacagatt acaacaaata ataaatactg
    18961 ctgaaaagtt taatagaaaa gttgctatat caggtcgctc aatggtcaat gtagtcggag
    19021 ttgcaaaaga attaggatac ctagatattt ctgatgacat gcttatagat ttaaatgata
    19081 tatgtaagta tgaagatagt gaattagtga taataacaac tggttcacaa ggtgaaccta
    19141 tgtcagctct tgctagaatg gctttttcag agcacaaaaa agtagaaata aagagtggcg
    19201 atttagttat aatatctgct catccaatac caggaaatga aaaattaata tcaagagtaa
    19261 tcaatttctt atttgaaaaa ggagcagaag tagtatacag tgatatagct gatatacacg
    19321 tttctggaca tgcctgtcaa gaagaattaa aacttataca tgcattagtt agaccaaagt
    19381 tttttatgcc agctcatggt gaatatagaa tgttaaaaag acatgctgaa atagctgaac
    19441 agcttggtat ggataaagaa aatatttttg tcatgcaaac aggagatgtt ttagaattag
    19501 ataaaaactc tgctaaggta gcaaaccgta tacaaacagg aaacatatta gttgatggtc
    19561 taggagtagg cgatgtaggt aatatagttt taagggatag aaagcattta tcagaagatg
    19621 gtcttatgat agttgtagtt actatttcca aggatgaggg taaggtctta gcaggtccag
    19681 atataatttc cagaggattc gtatatgtaa gagagtcaga agatttaatg gatggagcaa
    19741 aggatataat taagaatgtt ctaaatgaat gtgaagagaa aaatattaaa gaatgggctt
    19801 atttaaagaa taatattaaa gaaaacctaa aagaatattt atatcaaaaa actaaacgaa
    19861 atccaatgat acttcctatc ataatggaag tataatataa aaaaactata aatttgaaaa
    19921 ttagacctct tgtaattttt aaagtaatat caaaaatcca attgatatta tcttaagaat
    19981 tacaagaggt ctttttaata ctcatttaaa aaaaatatat attacataaa ataccaatta
    20041 taacaatctt ttattagtaa aaactactta tatataaatt ttactgatag gaggattctt
    20101 atggatgatg tttcaagaca aaatgctata aaagctttaa aacaaacaaa aatggaaata
    20161 gcaggagagt atggaatgaa ttatgaagat gcttttgaaa taatagaaaa tgcgtctaat
    20221 aaaggtgttt tagaaggtta ctttaaaaag cttgaaaaga aaaaaaattt aggtcaaggt
    20281 atatctaggc atttagaata aaaaaagtta aatgtgagaa aataagttta aataaacttt
    20341 aagaggtgac atcgatgaaa aaattagcta gtactgcttt ggctatatta attgcgctta
    20401 ctcctttaag cttttctttt gcaaataata aggaaaatgc tgatgccaac caactaaata
    20461 tatcttcaaa atcagcaatt ttgatggatg ttggtagtgg acaaatttta tatgaaaagg
    20521 atgctcataa aaagcttcct cctgctagtg taactaaggt aatgactatg ctacttatag
    20581 tagaagcttt ggattcaggc aagattaagc ttgatgatga agttcaagta agtgaaactg
    20641 catctagtat gggtggtagt caaatattcc tagagccagg agaaactcaa aaggttgata
    20701 cattattaaa aggtatagct gttgcttctg caaatgatgc ttgtgttgct atggctgaac
    20761 acttagctgg aagtgttgaa ggatttgttg atagaatgaa tgctaaagca aaagagttaa
    20821 atatgaatga tactcatttt gcaaatacaa atggacttcc agttgcaaac cactatacat
    20881 ctgctcatga tatagcttta atgtctagag aactattaaa acacgaaatg ataagtaaat
    20941 acttaactac atggatggat aaggttgtag tagggaagaa acaagttaca gtaggtcttg
    21001 caaatactaa taaacttata aagcattatc aaggagcaac tggtgttaaa actggtttta
    21061 ctcaagaagc aaaatactgt ttatcagcat ctgcaaagag aggtaatact catctagttg
    21121 ctgcaacttt aggagcagag acttctccag aaagatttaa tgatgcctct agccttttaa
    21181 actatggatt tgctaattat gaaagtgtta agttatgtag taaaggtgat aatatagcaa
    21241 cacttacatt ggataaggct gatgaaaaca aagttaaatt ggttgctaaa gaagatttaa
    21301 atgctcttat taaaaaagga agctctaaag aatttgagaa gaaaattgaa atagtaaaaa
    21361 atcctaagat gccaattaaa aagggaactg ttttaggaaa aataaagata tgtaaagata
    21421 aaaaagtaat aggcgaagtg gaattgataa atactaagga tataaataag gcaagctatc
    21481 tacaaatgct tcaaagaata attgataata tgatataaat aattatcaga tgaaatataa
    21541 ttataataca caaagatatg attataatac acataaaaaa tacccttatg tagttatttg
    21601 ccataggggt atttttattt accatataga agtattttat aatcttatga tttttataga
    21661 aatatagaaa agtttatgta tcttaaaata tcatttgatt taatatcata tatagggttg
    21721 tttaacaatt atctaagtaa aggcatacta ccagtaagtt tattcacttt ctttttattc
    21781 ccataaattg ctatcccaaa atactgtaac tcatcttctg gaacactagc aatcttttgt
    21841 gtaaattctt cataaagatt gcaactttgt gctacatctg aaaaatctac aacagttaaa
    21901 tcgttaaatt ccaaagtata taatttttta cgtaaatctt ttattatttc tttgtttcct
    21961 tttaaaatag gtacaggaat gtcaataatt cctaagtggc tattacctgt tttgtcaatt
    22021 acatctggcc caacagtttc tggggcatgt tttcctagtg taattcccat tatagctgct
    22081 gtattggata taattcccat tggtaaattt tcgtctatta ccatgacaca ttttgaattt
    22141 ttattttcca tttttatttc tcctttttac cttatttaaa gttgtttgaa tcatcattta
    22201 taaatatatt ttgatactgc tttggtgtaa gaccaataaa attcttaaaa aagtttgtaa
    22261 agtgactttg gtctacaaat cctgtttgta tcgcagcatc tataggttct acgccttttt
    22321 ctaaaagctt tttagctttg tttacccgta tattttctag gtatctatat ggtgtaatac
    22381 cttttaattt cgtaaaatta cgaagtagag agtacttatt catacctgct atagtgctta
    22441 attcgtcaag tactatatgt tcagcataat tattttctag ataatcacat actgcttgaa
    22501 tttctatatt tgtattttcc aagttagatt gcaaatttgg ctctgtatgt tcttcaatta
    22561 attgctcaat taggaagaaa aaaagctctt cctttttaaa atccaattct tcttccatga
    22621 ttatatagtg taattcttgt agtagaggta ctagctcact gcgaaatata acaggttgat
    22681 taaattttgg taaataattt ttacctgtaa tttcaaagac ggtttttttc attatttctg
    22741 gcttaatatt gatacatctg taatcaagta ctttatcatc tatttgttca caagtatgac
    22801 tgtcaaaagg gttaaaaagt agcaaatctc cagtacttgt ggtatactct ttatttttac
    22861 aagataaata tctttgacct ttttctataa aaccaattac ataatgttca tgaaaatggt
    22921 ttgggaattt ttgcattatg cctttaaatc tatatgcttc aatttttaat tccctatcaa
    22981 agcatactgt gcgttcttct tgtctcactt aggtaacctc ctttcatgtt atacaaagtg
    23041 taacacatat ttttttatac ttcttgtata atattgacta agagacaata atgtcaattc
    23101 atgtcaatat aatattacac ttacagtagg tgtaattatt atattttcac aatgaatatg
    23161 atataataat aaaagttata aaataaaaag caaattttaa ttttatgcct agaagttaaa
    23221 attattataa tgtaaaataa ttttgattat aagctattta attgttataa taatattaaa
    23281 aatttaattt aggagatgta cattaatgtt aagtatgaat ataggagaaa tagtagcaag
    23341 tttagttggt atagctatgg caatatcaat acatgagttt ggccatgctt attcagctca
    23401 cttattggga gatgatacag ctaaagctta tggtagaatg acattaaatc ctgtaagaca
    23461 tgttgatcca ataggtcttt tggctatgtt tatccttaag atagggtggg caaagccagt
    23521 accagtaaat ccaaataatt ttaaaaacta taagatagga aacctgatag tttcacttgc
    23581 aggcgttttt tgtaatgttt tgactgctat actgtgtgta attatcaata agtatgtgca
    23641 tatgtatgct ataaatacaa tagctggata tgtgcttata tatagtatgg gatttgcagc
    23701 ttttaactta cttccgatac ctccacttga tggatgggga gttatatcta catttgtacc
    23761 atataaatgg aatgaatatt tgtataaatt tgaaagtatg agttatataa ttttattaat
    23821 tgctttattt actggtatat atcaaataat actatcacca atatatacag tgttgctaaa
    23881 aatagtatat ttatttgttt aatataataa acagattaaa gagggattga tatgaagtat
    23941 aatatacaat tacaagtcta tgagggacca ttagatttac tttatgattt aataacaaag
    24001 cataaaatag atataaagga tatatctata atagatataa ctaagcagta tctaaattat
    24061 ttgaaaatgc tagataaaat ggatttagag ataactagtg aatttataac tatggcatca
    24121 aaacttttag agataaagtc taaatatctg ctttataagc aaaaagatga agaagaagac
    24181 ccaagaatag aacttatgga aaagttagaa gaatatagaa agtttaaggt agcatcacaa
    24241 gatataaaag aaaatataac ttatgttaat gagagatttt atagaaataa agaagagata
    24301 attattgatg acaatgtgga tttagaagat atatcaattg aagcaataaa aaatatactt
    24361 ccatacatat ttaaagttaa aacatcacag atagaaaatg ctaatgatga gaaactagac
    24421 aaaatagtaa ggaagaagat aatatctgtg gaagaaaaga tattgtatat aagagatatt
    24481 ataaaagata agattgaagt tacttttaca aatattataa aatcatatga aaatgatgaa
    24541 ataatagcta cttttttatc tatattagag cttataaaag aaaaagaaat agtagtagta
    24601 caagacatat tttttgatga tattctaata agaaaaagtt cggagtgtta aaatgaaaag
    24661 agaagatata aagtatatta tagaatctgt tatgtttgca tatggggaac caataagtat
    24721 aaaagaatta aactatataa taaataaaga actttcatca aaagaaatag agattatgtt
    24781 aaatttactt attgaggagt acagagaaca aaatagaggt attcaaataa tcaagctaga
    24841 gaataagtac caaatgtgta caaataaaga ctatgcagaa tatataaaga agattattga
    24901 accaaagaaa aagaaaagtt tgagccaagc tacattagaa actcttacta taattgccta
    24961 caaacaacca ataacaaagg tagaaataga ggatataaga ggagtaaaat gtgataaggt
    25021 attacaaact ttgtttgaaa atgaacttat aagagaagct ggtagattga ataagatagg
    25081 gaaaccaata atttataaaa caacagatga atttttaaag ttgttgaata tagaaagtct
    25141 tgaagaattg ccgcctatag aaaattatca agaagtggca acaaatgaat aaaaatataa
    25201 aatcacaata atttagtatg taattttaaa ttttaaaaga tttaaaatgc tctaagttgt
    25261 tgtgtttttt tgtataaata taaaattata attctcttaa aataaatatg gataattaag
    25321 tataaatagt aattttttga tgataatata aacatggagc ttaatgaatt atttaatttt
    25381 aagtatttaa taatagctgt aatttttatt ataagtgtaa ctatgttgat tttaatatct
    25441 catataaaca tcttaataat agctgatata aacaacaaag atatttgttt aaagcttaat
    25501 ataaaataca tgtttaattt aataaatata aatagacaac tttatccagc tgaaaatagt
    25561 aaaaataatg ataaaaaaga ggggatgaag aataatatag attcaagtat attattagct
    25621 gatgaccttt tgagcattta tagattatta aaaaagataa aaatacatga actttattca
    25681 aatattaatt ttggaactgg aaatattggc ttaacatctt ctgtatatgt attgataaat
    25741 actttgtatg gaaatttatt taatatgatt gatgcagaaa aaatgtattt aaatgtaaat
    25801 ccagatttta caaaagatta tgttttagga aatataagga tacatataag gccaagaata
    25861 aaagctttat ttaatattat aataatgatt aataaaatta tgaataaaaa taaaggaaat
    25921 aaggaaggtg atagtaatga gagcaacagg ttcgatacaa agtcttatgg aaacaactct
    25981 tgaaacaatt aaaggttcta tagatgcaaa tactattata ggagacccta taaaaacaga
    26041 tactacagtg gttgtaccta tatcaaaggt tacaattggc tttggtatag gaggaggaga
    26101 atactcaaaa ggatatgatg ataaagatag ggaaattgaa ttaaaaaatg aaaaatcaga
    26161 tacgaatttt gctggaggta gcgcaggggc tatatctgta caaccagtag catttgtagt
    26221 agttgaaagt ggagagacta gaataatgag tttagacagt aatatcaatc tagtggataa
    26281 tatattatct ataactccta gagtattgga gaaaattcaa aatatatcac aaagtaataa
    26341 gaatcaagat aaaaacaata tgtaagaata aaatattttt aaatatgtat atagttttaa
    26401 gtaaagtagt ggggttagta taaatcccac tattttgttg tgtactttaa acatttatat
    26461 ctaataagat tttagtttaa aacaatttaa atagataata aagttgatat atagtataat
    26521 tataaaaaga aaagaagtat tatattatag atatataaac gcatttgaga tataagaggg
    26581 agttataaat cattagtttt gaggtgaatg ttatgagcga taaagaatta tctattttgt
    26641 tgttagaaag tatgaataat tttcataatt taataaagat tattaataac gaaagatata
    26701 aaaaagataa ggttcttaca gaaagacagt tctttgcctt agtaaaaata agaaaacatg
    26761 ataaaataga gcttaaaaat ctaagtagag atttacatgt atctacttct agtctgtgta
    26821 tactacttaa taaattggtt gagcaagagt atgtatatag ggaagaagat agtagagata
    26881 gacgaaatac tttttatgga ataactaaaa atggagaaaa gatattggat aatgaaatac
    26941 taaagtttgt atctataata agtgataaaa tggattgctt agatatagat aataaagata
    27001 aattatttac ttctttggaa gagtctaaaa atataataga acaattattt taaaaaggag
    27061 aatttattat gaatataaag tattctaaag agatattaaa aagtttagaa aatgctataa
    27121 cagtagtagc tctatttgag ggagaagatg tttctacgac atttgataaa ataaatatga
    27181 taagcagaaa ttttaattct aaagaaaata gtatagatac tgtaacattg gttgaaaata
    27241 acaacttggt aaatgtttgt cttgttggtt ttggtaagag agagttattg aatagagaaa
    27301 aaattagaat cataggtggt aatttgtgta aaaaattaaa aaatatattg aaaaatgaag
    27361 catataagag tagtgatgtt aatgtactta atcttaattt aaatagtgat gaaataggtt
    27421 cttttgtaga agggatatta cttggaaatt ataagtttga taagtataag acaaaaagta
    27481 aggattttga aagaaaagaa gttgatacta taactatatt tactgaaaaa gaagttgagt
    27541 gtcaaataga aaaggctaaa atattagcta attcaactat aatagctaga aatctagtta
    27601 atgaaccatc aaacgtaata tatccaaaaa cattagcaat agaaacagtt aaacttggtg
    27661 cagagttcgg gtttaatgtt gatgtttatg aagaagaaaa aataaaggca ttgggaatgg
    27721 atgctttttt cgccgtttca agaggttcaa tcaataaacc aagattcata gtaatgagat
    27781 actttggaga taaagaaagt acagatatat taggtctagt aggtaaagga ttgacttatg
    27841 atactggtgg atattcttta aaatcaaatg cttctatgct agatatgaag acagatatgg
    27901 ctggtgctgc gagtgttata ggtgctatgt gtgctatatc tcaaagtaaa ttaaagaaaa
    27961 atgttatagc agtagtggct gcatgtgaga atgctttatc aggaggttca tacaaacctg
    28021 gagatataat tagttctatg gcaaagaaaa ctattgaagt tttaaatacg gatgccgaag
    28081 gtagacttac attagctgat gcaatatact acattataaa caatgaaaaa gtaacaaagg
    28141 tagttgatgt agctacatta actggtgctg ctttaacatt gttaggaaat gttgcaactc
    28201 ctatagtaac aaataatgat gatttttatt gtgaattaga aaaagctgcc actttatcag
    28261 gagaaagggt atggaagatg ccaatttatg atgaatttaa agatatgata aaaggagaag
    28321 aagcagacct taaaaacact ggtggtaaaa atgctggatg tataacagca ggagctttta
    28381 ttggtgaatt tgttgggaat actccttgga tacatatgga tatagcgggg acaagtacat
    28441 catcaaaatc aattggatac aaagcaaagg gggctacagg agaacctgtt agaacattat
    28501 actatttggc agaaatctaa aaaacaatta taaaatataa tttataaagc cacatggata
    28561 tttttgtaat ttaagattat ctatgtggct ttatttttta tttaagtaaa ttatttactc
    28621 tttttctttt tctcaagtat aaaagtatct ctattaaata aaagagcagg tatgtacata
    28681 atactagtaa caaaaataaa cgtaaaaata gcagttgtgc tatgacttaa aaaaccataa
    28741 tagtattgtg agttggtaaa tgtgtttagt aaaaatatga atgaaaaata aataaaacat
    28801 atagctaagt tatctctaag tgcatatctg ttaactctaa attttctaac atcaaccata
    28861 tatatatctg cataaattat caagcactta gagaagataa atataaaata tattaagcta
    28921 ggaaattcat agcccaagtt tttagaaaaa atgagttctc caactgaaca aactaaactc
    28981 caagaaaaaa atatattaaa aagatgatac tgcttaaaaa ttagtatggc tatacacaga
    29041 tatatacaga tataattgat tccaattggt aaagagttta gagtactata attacctgaa
    29101 gaaataagta ataattgctc tagagccata attattacaa gtaatgtaca aattatcttc
    29161 tcaactgtat agctataagg taaaatattt ttggttagtt ttggacaata gtataaaaat
    29221 aatccaaata taaagagact tacaaattgt tcactagaaa aaagaaaaat tttaaacata
    29281 taacattctc tcctataata aatttaatat gtatctatat aaatatattc attatatctc
    29341 aatttaatat ataaaaaaga taataatagt tagacttttt atatgaattt taataggtaa
    29401 aatatcctat tgttttgtag atatatccta attatgtcaa aaaataaaaa aatttttatt
    29461 tattagagtt tttggaaata ataataacag atattataat taattaacag tttttgtaag
    29521 tatttgacaa tagagagggt gtaacaaatg ggaataatag agtcagcatc aaaattggct
    29581 gagatggttc atttattggc tgttgaaaag gggataactg atatagaagc atgggatgaa
    29641 gctgtgaaag aatacagcaa aatttatgaa gaaagaagaa atgaatagaa tgataaaaaa
    29701 tgaaaacttg atgataaaaa ataactttat ttaaagaaat tcaattttta tagaaatatt
    29761 aaaaataata taaataaaaa gatggtattt caaatgtaag taaaaaatca ttttgggata
    29821 ccatccttat tatttatttt aataatatat aagtaataaa tcttacttat ccatattttt
    29881 tatgtaacct cttttttcta gcttttttat atgctcctta cgtatttcat caagattaat
    29941 accatattgt tcttctagct taaacatcat agtaacacat gtctgagcta catccattag
    30001 ttccttagca gttttcttta taatatcaac ttcatctaaa tcattatttt ctccacttag
    30061 atttctgaat ttacctatac attcagctaa ttcaccttgt tcttcaccca ttttgataaa
    30121 tgtagattct agtgttgggt ctaaattatt aagctctggt aaagatatag tttttagttt
    30181 cataaaagct cctttcaagt tattcgtatt tatactaaaa catcataaca aaaataatac
    30241 taaaaaaaaa ctttaaattc aacataaaaa cttacatata taataaaaat cgtgaaataa
    30301 atctatattt gtcttaatac agcctcatat aaaacttctc caataggttt atttattaca
    30361 agagaagctg acttatcata gactgtattt gctttattaa taagagcaat ataatcacct
    30421 ttgtaataat ctataaaact tgctgctggg tacacagcta atgatgtacc tcctataatc
    30481 aataaatcag cattacttat agcagaaatt gttttagtaa tgacatcact gtcaagagct
    30541 tcctcataaa gtactacatc tggttttact atagaaccac agttgtcaca ataaggaata
    30601 ttacctccca gatttaacat agattctaag tcaaaaaact taccacattt agtacaataa
    30661 tttctatgta cagaaccatg aagttctagc acatttttac ttccagccat ttgatgaagt
    30721 ccatcaatat tttgagttat gacagcttta agcttaccca tttcctcgag ttttgctaaa
    30781 gctatatgag cattgttggg ttttgcatta ggatatatta atttatcctt ataaaattca
    30841 aaaaattctt ctggatatct aacaaaaaaa gtatgtgaaa ctaattgttc agcagtaaat
    30901 tgtttattga gtttttggct aaataatcca gtagaacttc taaagtctgg aatattagat
    30961 tcagttgaaa cacctgctcc accaaaaaat acaatattat tgtgattagc tattaagtct
    31021 tttaagctat ttgcatccat agttacacct ccaaattatt atatataaat tgtactacat
    31081 attaggtctt ttatgtttta ttaatttaaa taaatatgta gtttaataag ttaaataaag
    31141 ggaatataaa taaaatgtat acagagtata aatagacaat atttactaaa ataacaagtt
    31201 gtcgtataat tatattaaaa ttgttttagt gtataagaag ggcgggggaa gtacgtgaag
    31261 aaaaaagcag ctttagcaac attggctatg ttacccttag gtgtggtaaa tgcacatgct
    31321 gatggagata taggtatagt gactataaat tatttaaatg taagaaatga gccaactgcc
    31381 gaaagtagca tagcctttgt tgctaaaaaa gatgataaag ttttgattaa agactcttct
    31441 aatggatggt ataaaataaa agctgaatct ggacaagaag gttgggcttc atcaaaatat
    31501 atagcaaaat caaatagtga ctctttaaga acgtctacaa ataaagaaaa acaagttatt
    31561 tcaaatagtt taaatatgag aaatggagca ggtacaagtt acagagtaat aactgtacta
    31621 aaaaaaggtc aaaaagtaga agtaatatca gagagtaatg gatggtctaa aatcaaatat
    31681 gatggaagac taggatatgt atctagctct tatttaggag acgtttcaaa ttcaactaat
    31741 aaatcaaaaa ctaaacaagt gaatacaact tcactaaatg taaggagtgg accaaataca
    31801 agttatggtt tgttaggcaa gttgccaaaa ggaagtaaag tagaagtaat atcagaaagt
    31861 aatggatggt ccaaaataaa gtataatgga aaagatgcct atatatctag tatgtactta
    31921 tcggatgtaa gtcaaagtaa ttcagacaat tctagtcaaa gcaatgataa aaagaatact
    31981 gataaggttg taaatacagc ttctttaaat gtaagaagtg gaccaggttc tacatatagt
    32041 aagttgggaa aagtttataa aggaagtaaa gtaactgtac tatcagaaag tagtggatgg
    32101 gctaagatta attttaacaa taaagaagca tttgtagtag gcaattattt atctacttca
    32161 gcagatactt caaataataa ctcaaatagt aactctgata acagttctaa tagtaatggc
    32221 aataattctt catcatcagg tcaagtaaat ggtatgtcag gtataagtgg ggctaaaatt
    32281 gattataaat ctttgagtta tactttagaa tctcatataa gtaaacaagt tgagaaagca
    32341 gcatcaggag gaaatgtgat agctccaagt aataggaaaa gcactccaag tcctgaattt
    32401 agtacttttt cagctcaaag aacaagctca tttgtaaatg caagttcaag tgatatagaa
    32461 tattatttaa atcctaaaaa ttttacaaat actactaaag gtatgatgca gtttttaaag
    32521 attaacagtt atagagatgg tatttcagaa tcaagtctta actcgtatct taatggttta
    32581 tcttctagtg tatttaaaaa ccagggagca gcctttataa atgctgccaa gaagtataat
    32641 atagatgttg tatatctagt atctcatgct atgtgggaaa ctgcttatgg taagtctaca
    32701 cttgcccaag gtcaaacatt aacatcttat aagggacaac ctcttagtaa gccagttaaa
    32761 gtatacaatt tttttggtat aggagcaata gataaaagtg ctaatgtttc aggtgcagaa
    32821 gcagcgtatt ccaatggctg gactagtgta gaagctacaa tagatggctc tgcaaagtgg
    32881 atatcacaaa attacgtaaa tagttctaag tacaatcaga atactatcta caagatgaag
    32941 tggaattatg attatacatg gcatcagtat gcaactgatg taaactgggc taatggaatt
    33001 tctggaatta tggaaaattt aattggtctt tatggaggag gaagtagttt ggtttttgaa
    33061 gttcctcaat ataagtaata cataaataaa aaatatagac tatgggtagt tttcatttta
    33121 tttaaattat ctatagtctt tttgtatata atttataatc tgtaaattta atgttaacaa
    33181 aacttttatg ttataatatt aattctaagt aaatataagt gagtaatagg tagaaataga
    33241 taaaaagtgg taataatata tataaagata attttgagaa aggaggatat atatggaaag
    33301 tattaaggaa tatttggata agttagaaat taataatagt ggtcttggaa agcaacttaa
    33361 agaagtttat ataaatagag tagtttattt taaggaagat aagatagttt atttttatct
    33421 aacttctaaa gatattgttt ctcatgaact tttagataaa tttaaagaag aattgatgta
    33481 taaactcgat tattttaaag atatgaggat gaagataaga tttactggtc ttgaaagaaa
    33541 atccaataaa gatgttataa aaaaatattg gaataatata ttgtatatat taaagtatct
    33601 ttgtccatct attgcagggt ggtataaaca agtagaattt ctttgtttag aagaagaatt
    33661 aaaaataaaa cttccaaaag gtatatttta tgagcgatta atgaaaaaaa atgtaactta
    33721 tgtacttaaa accgtgctta gcgaagagtt gggattagat ttaaatataa caatagaaaa
    33781 agctgttgat gaaaaggtaa ataaagaaag actcataaga atcaatgata gagaaatgga
    33841 agaaaaaatt agggctttag aaattggaaa agttaataac tgtgaagaaa atgaagagga
    33901 aagctatgtc attaagtctg atgtagatga aaatttaatc tatggagata atgcaaatgc
    33961 tatgattgaa aatatagttg aattaaatgc atcttctgga acagttgcag ttgttggaga
    34021 tatatttgat gttgacacta aagaacttaa aaacggaaaa atacttatga tagcttcaat
    34081 tacagattat actagctcaa taagctgtaa acttttttta acagatacaa ataaggatgg
    34141 agtacttgaa aatgttaaaa agggagcata cttaaaaatc aagggtgaca tagtttatga
    34201 tacatatcaa agagaaatta gtatgatgat tagtgggata agaaaagaaa ctagaataga
    34261 gagagaagat aaatcagagg aaaaaagggt agaactccat gcacatacac aaatgtcttc
    34321 tatggatgct atttgttctg ttaaaaagct tgtagagcgt gcagccaaat ggggtcattc
    34381 tgctattgct ataacagacc atggtgttgt acaaggattt ccagatgcta tgaatgcagg
    34441 taagtcaaat aatataaaaa tactttatgg agtagaaggg tacttggttg aagatgattc
    34501 tctcattata gaggatgcaa atgataaaga actttcgcag acatttgttg tatttgacat
    34561 agagacaact ggtttttcta atacaaatga taaaataact gagattggtg ctgtaaaaat
    34621 agaaaacttt aagattgtag atagatttag tgagttaata aatcctgaaa aagatatttc
    34681 ctataagata caggaattga caggaataac taatgaatta attaaagaca agccaaccat
    34741 tgaagaaatt cttcctaagt ttatggaatt tgttggagat agtgtattag tagcacataa
    34801 tgcagagttt gataccggat ttatatcgca aaaatgtagg gaacaaggct tgttatataa
    34861 taacaaaaaa gtagatacac ttatgttagc aagagtaatg ttgccaaact taaaaagaca
    34921 tagattaaat gtcgttgcaa aagaacttgg tattcctctt ttaaatcacc atagagcagt
    34981 ggatgatgct gaggctacag cacttatatt taataagttt ttgcagatgc ttacagaaaa
    35041 aggtgcaaaa actctaagtg atgtaaacaa tatacttgga aagattgatt acacaaagct
    35101 tagcacaaat catataacct tgatagcaaa aaattctata ggtataaaaa atttatataa
    35161 aataatttca gatgcacatg taaatcattt ttttagagca ccaagaatcc ttagaagtgt
    35221 attaaatgaa tataaagaag gattaataat tggttcggct tgtgaagctg gggtggtttt
    35281 tcaagctgta aagaaaaacg tgtctgatga ggaaatgaaa aaaataatag atttatacga
    35341 ttatattgaa gtaatgccta tagataataa tagatttatg attgataaag gagaagtaaa
    35401 ggatgaagaa gagttaaggg agttgaatag gaaattaatt gatacagcca aaaaatttga
    35461 caaaattcca gtagctactg gggatgttca ttttttagac aaacatgaag ctgtcttaag
    35521 aaaagttctt aaatattctc aaggttttaa agtagatgaa gaagaaactt atttacattt
    35581 tagaacaaca gatgaaatgt tggaagagtt tagttatctt ggagaagaat tggcatatga
    35641 agttgtagtt gaaaatagta atctgattgc cgatatggtt gaagatataa aacctatacc
    35701 agatgatact tatcctccaa ttatagaggg ctcagatgtg gagttaagag aaatgtgtta
    35761 caaaaaagcc aaacgaatct atggagaccc aataccagaa atagttcaaa aaagactgga
    35821 tagagaatta aattcaataa taagcaatgg gtatgcagtt atgtatatta tagctcaaaa
    35881 actggttgca aaatctcttt cagatggata tttagttggt tcaagaggct cagttggctc
    35941 atcttttgca gctactatga gtgatataac agaagttaat cctcttccag cacattatat
    36001 ttgtgaaaac gaagactgta aatattcatt cttttatgaa ataggtgaat ggggctcagg
    36061 tgttgattta ccagacaagg attgtccaaa gtgtggaaga aaacttgcta aaaacggtca
    36121 tgatattcca tttgaggttt tcttaggttt tgaaggtgat aaggaaccag atatagactt
    36181 aaatttctca ggaaattatc agccaacaat tcataaatac acagaagaac tatttggaga
    36241 ggggtatgta tatagggctg ggactatagg tactgtagca gaaaaaacag cttttggata
    36301 cgcaagaaaa tatgtggaag aaaataatat aagtgtacca aatgcagaag tacttaggct
    36361 ttcaaatgga tgtactggag taaaaagaac ctcaggacaa catcctggtg gagttatggt
    36421 tataccagac tataaagatg tatacgattt tacacctata cagtatccag caaatgatac
    36481 ttcttgtggt gttataacta ctcactttga ttatcattca ataagtggta gaatacttaa
    36541 acttgatata cttggacacg acggtcctac cattataagg atgcttgaag atatgactgg
    36601 aataaatata acagaaattc ctttagatga taaagagact atgtctctat ttacatctac
    36661 agaagcatta ggtgttacac cagaagaaat aaactgtcct ataggttgcc ttgctgtacc
    36721 agagtttgga actaagtttg tacgacagat gttacttgac accaaaccca ctacatttgc
    36781 agaacttgtg cgtatatctg gtctctctca tgggacagat gtttgggtaa ataatgcaca
    36841 agacttagtt agagaagata tagttggatt aaaagatgtt atatctacac gagatgacat
    36901 aatgaactat ttaatattta aaggattacc tcccaaaatg agttttacaa taatggagag
    36961 cgtaagaaaa ggtaaaggtc taaaaccaga acacattgaa gaaatgaaaa aaaataatgt
    37021 tccagaatgg tatataggct catgtaaaaa gattaagtac atgttcccaa aagcacatgc
    37081 tgttgcttat gtaatgacat catttagaat tgcatattgt aaagttcact atccagaagc
    37141 attttatgcg acttatttta cgacaaaagt tgaggatttt gatgcagatt taattgttaa
    37201 aggattagat gctataaaat caaaaataaa tgaaattgag tcactgggaa atgatgcaac
    37261 cacaaaagaa aaaggtatgt tgaccgtttt agaagttgct cttgaaatgt atgctagagg
    37321 aataaaatta ttaccagttg atatatataa atcagatgca actgagttta tagttgtagg
    37381 agaaaaaaca ttacttccac caatggcagc aatacaaggg cttggaggaa atgcggctat
    37441 aaacatccaa aatgaaagaa aaaatggtga atttatatca aaagaagatt tgagaaagag
    37501 aacaaaaata tcaaaaacgg taatagaaac tcttactaat catggttcac ttgagaatat
    37561 gagtgagaga aatcaattgt cattgtttta agttagccca aaaagttgca aataaaagaa
    37621 ttttatgata tcatagtata gaaaatagta tactttgcag aaactaagag tgagacagct
    37681 gttctcactc ttttatttta aatatattat taaaaagttg gtttattttt attaaatata
    37741 ttttatttaa gattatataa actaatcatt tacaattgaa taggaggaac tttaatgaaa
    37801 aaaaatttag aagctacaat tgaggagatt gtaactaaaa taacggatga acatggattt
    37861 gaaatggttg atgttgaata tgtaaaagaa gcaggagaat actacttaag agtatacata
    37921 gataaagaag aaggaatatc tttaaatgaa tgtgaattag ttagtagaga attaagtcca
    37981 atactagatg aaaaagaccc tataaaagaa aattactttt tagaggtatc ttctccagga
    38041 cttgatagag cattaaaaaa agacagagat tttgttagat atcaaggtag agatgtagat
    38101 ttaaaattat ataaaccatt aaatggatgt aagcaatttg aaggtgaact tgtaggatta
    38161 acagaagata ataatataaa aatcattgca aatggtaagg aaatggaatt taatagaaaa
    38221 gatgttgcta ttgtcagatt agcaataaag ttttagtggt atattaaata gggaggatat
    38281 aaaaaatgaa tcatgaattt atggaagcat tagatgaatt agtagttgac agaggtatag
    38341 ataaagaaat acttatagat actatagaac aagctctttt aactgcatac aagaaaaact
    38401 ttggttcagc acaaaatgtt agagttgaat ttgatagaga caagggagat ataaaagtat
    38461 tttctcaaag agttgttgtt gatgaatcag atttatatga tactttctta gaaatagagt
    38521 tagcggatgc tagagagata aaccctaatt atgaattagg tgatattata gaacatgaag
    38581 ttacacctaa aaactttgga agaattgcag ctcaaacagc aaagcaagta gttgttcaaa
    38641 gaataaggga agctgagaga gaaatagtgt ataatgaatt tatagaaaaa gaaaatgaaa
    38701 tagttacagg agaggttgct agagcaaata aaaatgttgt ttatgtaaat cttggaagaa
    38761 tagaaggtat aatgactcaa agtgaacaaa taccatttga gaattataaa gcaggtcaaa
    38821 aaataaaatt ctatatactt gaagttaaaa agactaacaa aggacctcaa atagttgtat
    38881 caagaagtca tccgggtcta gtaaagagat tgtttgaaga agaagttcct gaaatatatg
    38941 aaggaatagt tcaaataaaa tctgtagcaa gagaagcagg gtcaagaact aaaatggcag
    39001 taaaatctat agatgaaaaa atagacccaa ttggagcttg tgttggacca aaaggactta
    39061 gagtaaaaaa tatagtggat gaacttggcg atgagaaaat agatataata aaatatagtg
    39121 aagacccagc agagtttata tcagcagcac ttagcccatc aaaagttgtt agagtagata
    39181 tagatgaaga tgaaaagtct gccttagtaa tcgttccaga ttatcagtta tcgcttgcta
    39241 taggtaaaga aggtcaaaat gcaagactag cagcaaaatt gactaactgg aaaatagata
    39301 ttaagagcga atctcaagca gaacaagaaa tgttaaatct tgaaaattcc aaagaaatag
    39361 aagctgagga tgttgaagct gaatttatcg accaagaaga gttaggtact tcagaggata
    39421 attttagtga agaagagtaa ggagggatga ttttgaaaaa agtaaaaaaa gtgcctcaaa
    39481 gaaaatgcat agcatgtcaa gatagagact ctaaaaaaga gcttataaga atagttaaaa
    39541 ataaagaagg aaaaatattt ttagacccaa caggaagagc caatggaaga ggagcatatg
    39601 tatgtaaaag ctctgaatgt ctaaaaaaag ctataaagag taaagcatta aataaagctt
    39661 ttaaaataga tgttccagat gaagtctatg ataatctact tatggagtta gaagaatatg
    39721 aaaaataata aagaaaaagt actttcgttc ttagggctcg caactcgtgc agggaaaata
    39781 gtgtctggtg atgattcaac tttgcttgac cttaaaaaag gaaaagtaaa tttgatttta
    39841 atcgcagaag atgcatcaaa taatactaaa aaacttttta aagataaatc aacttttaga
    39901 aatattccat atctattttt ttcaacaaaa gaggaaatag gatttgcaat aggaaaatct
    39961 ccaagagcag ttgtaggaat aaaagatgaa aatttttcta aaaagataat tgaactaata
    40021 gaaatttaaa ataaaatagg gggtgatctt gtgtcaaaaa caagagtata ccaaatagca
    40081 gaagaattaa atatatcaaa tgaagagctt ataaataagt tagctgaatt agatataaat
    40141 gtaactgaca aagattcagt attagaaggt gaagagttag aattagcact tgaaatgtta
    40201 ggtgaagatt tatctcaaga aaatggaaat gttatagaaa tagatggaaa gttgacagta
    40261 caagtattag caacgaaatt agataaatct ccatcagaaa taattatgaa gcttatgaaa
    40321 atgggaacta tggcaacaat aaatcaagag ataagttttg agattgctgc tttggctgcc
    40381 aaagactatg gatttgaatt aacagtagct gaaagtgatg atacagaagc tttagaaata
    40441 gaagctttaa tggaaattga agaagataaa gaagaggatt taaaacctag accaccagtt
    40501 gttacagtta tgggacatgt tgaccatggt aagacatctt tactagatgc aataagaaaa
    40561 actgatgtga tttcaggtga agctggagga ataactcagc atataggtgc ttcagaagtt
    40621 aaaatcaatg gacacaaaat agtattttta gatactccag gacatgaggc tttcacatca
    40681 atgagagcaa gaggagcaca agttacagat atagcaatat tagtagttgc agcagatgat
    40741 ggtataatgc ctcaaacagt tgaagctata aaccatgcta aagcagcagg tgtaccttta
    40801 atagttgcta ttaataaaat agataaacca ggtgcaaacc cagataaggt taaacaggaa
    40861 ttagcagacc aaggattatt agttgaagac tggggtggag aagtaatagc agttccagta
    40921 tcagctaaga aaaaagaagg aatagataca cttttagaaa tggtattatt ggttgctgaa
    40981 atggaagagt taagagctaa tccaaataaa agagcagtag gaactgttat agaagctgag
    41041 cttgataaag gaagagggcc agttgcaaca gtacttgttc aaggtggtac tttaacagta
    41101 ggtgacccaa tcgttgcagg tgttgcatgt ggtaaagtta gagcaatgat aaatgcaaaa
    41161 ggtaagagag taaaaacagc aggaccatct acagcagttg agatattagg tctatcagaa
    41221 gttccacaag gaggagacca atttgtcgaa gttccaactg ataagattgc tagaagtgta
    41281 gcagcaagaa gacaacaaat agttagagat gaaatgttaa aatcaactca aagattatca
    41341 ctagatgctc tatttagtca aatgagtgaa ggttctataa aagaccttaa tatagttata
    41401 aaagcagatg tacaaggttc agtacaggca gttaaacaat ctttagaaaa attatcaaat
    41461 gaagaagttc aagttaaagt aatacatggt ggtgttggag ctgtaacaga atcagacata
    41521 ttacttgcag cagcttctaa cgcaataata ataggattca atgtaagacc agttccaggt
    41581 gcagaatctc taggagaaaa agaaaatgtt gatataagaa catatactat aatatacaag
    41641 gcaatagaag atatacaagc ggctatgact ggtatgttag accctgaata tgttgatgaa
    41701 gaaacaggaa aagctgaaat aagagaaata tataaaatat ctggtgttgg aacagtagct
    41761 ggatgttatg tgactaatgg taagatattt agaaattgta aagtaagatt agttagagat
    41821 agcataataa tacatgaagg tgagcttgca gcacttaaga gatttaaaga tgatgtaaaa
    41881 gaagttaatt ctggatacga atgtggtatg agttttgtaa attataacga cataaaagaa
    41941 ggcgatatag tagaagctta tataactaaa gaagtagaaa gaaaattata aattcgtatc
    42001 aaagaaaatc agttagtaaa gaaggtcgat ggtatggcat catataatag aacaagaagg
    42061 atagcagaag aaataagaaa agtcgtaagt actatgctta taaatggagt aaaagaccct
    42121 agaataacat ctatggtgag tgtaactgat gtagaagtta caaatgacct aagatatgct
    42181 tatgtatatg taagtattct aggtggagat gaagaatcca cactaacagg tcttaaaagt
    42241 gctggaggat atataagaag agaagtagga aaaaatataa aacttagata tattcctgag
    42301 atagtattta aacttgatga ttcaatagaa aaaggaatgt atatggataa ccttataaaa
    42361 agagttaatg aaaaaaatgc tcaacaaaat gaggatgaaa attatgatga ataatatgga
    42421 tagtattata aactatattt atgagggtga ctattttata gttacctctc atatcagtcc
    42481 agatggagac aatgttggtt ctacactttc aatgtattat acattgaaga atttaggaaa
    42541 aaatgtatat tatgtacttg atgatgaagc tccattaaat ttaaggtttt tggttgaagg
    42601 tgtaaaaatc tataagtcta atgaatttaa tatgaagaac tattctttaa ttgcacttga
    42661 ttgtggggat aagatgagaa tatgtgtgag tgatgaaatc aaaaataatg catcaaaatt
    42721 aatctgtatt gaccatcatg ctagtaatga ttcatatgga gatttaaatt atgtagatat
    42781 tgatgcatct tctacatgtg aactagttta taatttattg gttagatttc agcaaacaaa
    42841 agatattaat attataaatg aagatatagc aacatgttta tatacaggtc ttgtaactga
    42901 tactggaaac ttttcgtatt caaatgtaca tgcaagtagt tttgaaatgg caaaaaattt
    42961 acttgtttta ggtgcacaaa aaaatactat aatacaaaat atatatcaaa gcaattcttc
    43021 tgattattat aaattattag gagaagctct aaaaggtttg gagatatttg attcaaaggt
    43081 atctagtata gttttaactc aagacatgat gaatagaaat aatattagct ttaatgatgt
    43141 tgatggaata acaacatata caagagatat aaaaggtata gaagttggaa ttttatttaa
    43201 agagaaaaaa caaaatgaaa ttaaagtgag ttttaggtca aaaaattatg ttgatgtaag
    43261 tgaaatagcc aagttatttg gcggtggtgg tcatgtaagg gctgctggat gtactattag
    43321 agatagtatt gataatgcaa agaaaatggt actagaggca gtgttaaaat caatttaagg
    43381 tgacagtaaa atgaataaga ttataagtat attgaaaccc acaggtatga catctcatga
    43441 tgttgtaagt agagtgagaa agattttaaa tataaaaaaa gttggtcata caggtactct
    43501 tgacccagat gcttcaggtg ttttaccaat ctgtataggg aaagctacaa aagtatgtga
    43561 agttatttta aacaaagata aatcgtatat atgtgagctt actttaggta taagtacaga
    43621 tacttatgat gcttcaggtg aaattttaaa aaaagttgat gattttaaat ttagtaatga
    43681 agacatagaa agagcttttg atacacaaag aggagagata aatcaacttc ctccaattta
    43741 ttcagcttta aaggtaaatg gaaagagaat gtgtgattta gtaagaagtg gtagacaatc
    43801 tgaaattact ttaaaaacaa gaagagtaaa tataaaagac ataaaaatac taagtataaa
    43861 aggtaataaa gtaatgtttt atgttgagtg ttctaaaggg acttatgtga gaagtatttg
    43921 ccatgatata ggagaatatc tgggttgtgg agctcatatg tcgtttttga atagaacatc
    43981 ttctggtaaa tttgacttag ataattcaat aacattagaa gaattagaat tattttatga
    44041 aaataaaact ttagacaaat acttatatga tatagattat gtccttgatt cgtttaatta
    44101 tgttgtatta aaccctaatg ctataaaata ttatagtaat ggtggctcaa tagatgataa
    44161 aaggttttta aaaaataatt tcgataaaga tgatgaagtt gtaagagtat acagtacaga
    44221 taatttctta ggattaggaa agttatcaaa acacaataat acaataagtg ttaaaagtga
    44281 taagatgttc atataaccaa tgtatttaaa aaaacaacaa aagagatagt actataataa
    44341 cttctatagt ctatctcttt tacttatgct ataatagttt gtgaataagt taagagtaaa
    44401 ttatatttta gcggggaaca tttattatgg tgattataaa atctataaaa gaaatagcaa
    44461 acataaaaga aagtgtcatt accataggaa attttgatgg tgtacacaaa ggacatcaag
    44521 ttcttatagg aaaaacagtt gaatatgcaa aaaaagaaaa tatacagagt attgtattta
    44581 catttgcaaa tcatcctgta aattacttta gaccaaactc aacaaaaaat attataagca
    44641 atgatgataa aataaaatta ttagaagatt tgggaataga tatagttgta atcataccat
    44701 ttgatgagta tatgactaaa atacctgcaa aggatttcgt agatgagata ctagtaaaaa
    44761 aattaaaagc taaaaagata gtaattggac atgactttac atttgcaagg gcaaaagaag
    44821 gcaatgttaa tctattaaaa agcttagaac atgaatttgg ttttgaggta gaagtaataa
    44881 aacccatcaa aatcaatgat gtaagagtga gtagtactta tattagaagt ttagtatccc
    44941 agggtgatat ggctaatgtc aaggaatatt tgggtagaaa ttacaagtta gaaggttgtg
    45001 ttatacattc taaacacctt ggaagaacta taggattccc aacggcaaat attgacctta
    45061 agaataatat gctcgttcca aagagaggta tttatgcttc tattgtacat attggagatg
    45121 agacttattt tggtgctaca aatgtaggct ataacccaac agttaatgga aaaactttgt
    45181 ctattgaaac aaatatttta gagtttgata gagatattta tggggaaaat ataattgttg
    45241 aatttttgga aagaataaga gatgaaagaa agtttaattc aatagatgac ttaaaaaatc
    45301 aattgtataa tgatacaaat tatgtgtatg aaaactatgt ttgcaaaaac aagtaatgta
    45361 tgataaaatg attaaggaaa ataaccatta ctcggcattt gaatactcca atcaatgctt
    45421 agtaataggc gtttctaaaa aaatacggag gtaatctaaa tgataaacaa atcagaaata
    45481 ataaaagaat atggaagagc tgaaggggat actggttctc cagaagtaca aatagcttta
    45541 ttaacagcta gaataaacga gttaaatgga catttaaaaa ctcataagaa agaccaccat
    45601 tcaagaagag gactattaaa aatggttggt agaagaagaa acttattagc ttacttaaag
    45661 gaaaaagatc ttgaaggata tagagcatta atagctaaat taggattaag aaagtaattt
    45721 cattctttat aaaaaagcag gtatagtgta tacctgcttt tttatagtta attcattata
    45781 aagtaaaata gttaataata aaagtagttt atatgttata aaaaattata aaatgaataa
    45841 tattgggtaa gaatattaaa atagtataca aatacataat caatttaaaa ggaaagacag
    45901 gataaatata gaaattttat aacaggataa ttagttggaa taaggaggat attattttga
    45961 aaataaagtt agtatgcgat agtttgtgcg atattccaga tgaaatatca gaaaaagatt
    46021 atatagagat agttccactt acagttattt ttaatgatag ggaatatata gaaggagtag
    46081 atataaagaa agaagagttt tataaaaaag taaaagaaat caagcaaata cctaaaactt
    46141 cacaagctac atatatggag tttaaagaag tgtttgataa gttcataaca gaaggttatc
    46201 atataatctg tatgacaggg gctgcaaacg catcaggaac ttttcaaagt gctatgatag
    46261 ctaaaaatga tgtaaatgaa aatgaaaaaa tacatatatt tgatactaga aatttatctc
    46321 taggtagtgg acaatatgtt ataaaggcat gtgaacttct tgaagaagga ctgggatttg
    46381 aagaaattat agatgaatta gaaaatactc gcagtagtgt aagattactt tttgcaccat
    46441 atacacttga ttttttaaag cagagtggaa gagttccagt tgcaactgct ttgataggaa
    46501 atatgttaaa tataaagcct atatttttct ttgataatgg ggaagctaag ttggtaaata
    46561 aggttagagg aataaagaat atagcaagta aactggtcga tataatttta gaaatgaatg
    46621 aagggtcttt agaaggaaaa atcgtcacaa taggttgtgg agataattta cacgattgtg
    46681 aaatattaaa agatgaagta aataaaagaa taaaagcaag aagagtatta tttactcgag
    46741 gaggagtttc tatttgctct catacagggc cagatatatt ggctataagt tgttctaatt
    46801 agtagtaata taaggttttt gcatatatat gtgaagataa ttttatattg ttaatttata
    46861 gtatttttga taatatagta aatagagtga tatacgtgtt ttttaataag aggaggacaa
    46921 gcattaatgt ttgaacataa aatatttaaa atggattttg caggaagaga actttctgta
    46981 gaaataggaa agatttgtga aatggctagt ggtagttgta tagttcgata cagtgacagt
    47041 atggtaatgg taaatacaac taaatcagcc aaacctagag acggaataga cttttttcct
    47101 ttaagtgtgg attatgaaga aaaattatac tctgtaggta agatacctgg aggtttttta
    47161 aagagagaag ggaagccttc agaaaaagct atacttacat caagattgat agatagacca
    47221 ataagacctt tattcccaaa aggatttaga aatgatgttc aggtagttgc aacagttcta
    47281 tcagtagacc aagattgtac accagatata gtagcaatga taggttcttc aatagccctt
    47341 tctatatcag acataccatt taatggacca acaggttcag tatgtgttgg ccttgtagat
    47401 ggtgcatttg ttgtaaatcc aaacgcagag caaagagaaa aaagttcaat gcatctagtt
    47461 gtttcaggta caaaggaagc aataatgatg gttgaagccg gagctgatga agttccagat
    47521 gaggtaatgc ttgatgctat tttatttgct caccaagaaa taaaaaaaat agttgagttt
    47581 atagaaggta tagttgcaga agttggaaaa gaaaaaatgc ctgttgaatt ataccatgca
    47641 ggagaagaaa taactcaatt ggttcgcgaa tttgcaactg ataagatgaa aaaagcagtt
    47701 cagacttttg aaaagttaga gagaatggaa aatatggata gagttaaaga agaaacttta
    47761 gctcactttg aagaaacttt agaagatttt gaagattttg ttggagatat agaagaagta
    47821 ttacaagata taataaaaga agaagtaaga aaacttatag tacatgaaaa tgtaagacca
    47881 gataatagaa aacttgaaga aataagacct atatggtgtg aaactggcat gatacctaga
    47941 gctcatggtt cagccatatt tactagagga caaactcaag tacttaatgt tgctacttta
    48001 ggtgctttag gagatgttca aaaattagat gggttagatg aagaagaaaa caagagatat
    48061 atgcatcatt ataatttccc cgcatatagt gttggagaag ctagaccatc aagaggacca
    48121 ggaagaagag aaataggaca tggtgcttta gcagaaagag ctttattacc agtaatacca
    48181 tctcaagaag aatttcctta tgctattaga ttagtttctg aagtattaag ttcaaatggt
    48241 tctacatctc aagcaagtgt atgtggctca acattatctt tacttgatgc aggtgtacct
    48301 ataaaagata tggttgcagg tatagcaatg ggacttataa agcatgatgg taaggttgca
    48361 gtgctttcag atatacaagg tatggaagac catttaggcg atatggactt taaagtagct
    48421 ggaacagagt atggtataac agcaatacaa atggatataa aaattgatgg tatagataaa
    48481 gaaattctac aacgtgcttt aaaacaagct aaagagggta gaatccatat actaggagag
    48541 atgagaaaaa ctatatctca gccaaaacca gaactttcac catatgcacc taagattgtt
    48601 aaaatgcaaa taaatcctga caagataaaa gatgtaatag ggcctggtgg aaagataata
    48661 actaagataa tagacgaaac tggagttaag atagatattg aacaaactgg ggaagtattt
    48721 atatcaggta ttgagattga tatgattaag aaagctcaag aacttataaa taatatagta
    48781 gttgaaccag aagtaggaaa aacttataaa ggtaaggtaa gtagaataat gaactttgga
    48841 gcatttgttg aaatacttcc aggaaaagaa ggattacttc atatatctca tatagctcat
    48901 gaaagagttg ccaaagttga agatgtattg aatatagggg atgaagttga agttaaagtt
    48961 acagagattg atgaaaaagg aagagttaat ttatcaagaa aagttctttt accaaaacca
    49021 gaacataaaa ataaataact tattatataa ttaaaagcaa agtcttagga ttttgctttt
    49081 ttaaatacaa aaaagtagat aattttaaaa tgatagttat ggatggctta tagttctttt
    49141 tctgtaaggc tttactctta taaagaagct atttaaaagc tgattaatag aaagctttat
    49201 tgatatatat agaatcactt aagttatatt tagtgcataa acagagctta tttttaatag
    49261 tattaaataa gaaacaattt gtacataatt agggggagtt ttaatgataa tgatggtact
    49321 taataagagt aagctcaaaa aaatcataat actaatttta gtagtgttat tagtattatc
    49381 aggaggggta atggtattaa agttatttaa caacaagcca gtatttaatt tatttaatgg
    49441 tgtagattta ccatatgaaa gaggtacaaa ggataaaagt ggatatgttg cattaacctg
    49501 taatatagac cttggatggg aaacagagta cgtagaatcc attttagaaa cacttaaaaa
    49561 agaaaatgta aagattacat ttaatgtaac tggaaaatgg gcagaaaaga ataaggatga
    49621 attattaaag ataaaaaaac aaggacatga aataggtaat catggatata aacatttaga
    49681 ttattctaca ttatcatatg aagacaatta tgagcaaata gagacctcta aaaagataat
    49741 tgaagaaatt ataggtgaaa aaactaaatt ttttcaagca ccagcaggtt cctttggtcc
    49801 agaaacagta aaggctgcta aagcattagg gtatacttct atcaaatggg atgccgatac
    49861 tatagattgg aaatacaaag accaaccaga ggtaatcatt gatagaatga agaaaaaaga
    49921 cattaaagat agtagtataa tcttaatgca tccaaccaac gcaacgacaa aatgtataga
    49981 tgatattata gctattgtaa gagaaaaagg actaaaacca gggaaactta gtgatgtatt
    50041 taagtagaca aagtataaat atttggttaa gattgtataa gttgtagttg aattaattaa
    50101 aagactgact tataatgtaa tacatataaa aaataaatat aattaaaaca tagtgttttc
    50161 aataatccaa tatgaaacaa agaataaatt gttaggagaa tgtaatgtat aagacaaaaa
    50221 tactggaaaa tgggcttaca ataattggtg aagaaatacc atatctaaag tcaataacat
    50281 taggtatttg gataaatgca ggctctagga tagaagaagc acaggtgagt ggaacttctc
    50341 attttataga gcatatgatg tttaaaggaa ccaaaaacag aacatcaaaa gagattgcta
    50401 gttcaataga taatttgggt ggtcaaataa atgcatttac aagtaaagaa tgtacatgtt
    50461 attatgtaaa actaatagat gagcatatag atacgggaat tgatgtattg agtgatatga
    50521 tactaaattc taaatttgat aaaaatgata tagataagga aagacttata atattggaag
    50581 aacttaagat gtatgaagac tctccagatg acttatctta tgatttatta gtggaaaata
    50641 tatatgctaa tgatggatta ggaatgaata taattgggac aaaagaatct ctatacaata
    50701 taactagaga atccatgtta gaatacttaa ataagtatta tattccaaat aacgctgtta
    50761 tttctatagc tggaaacttt aactttgatg atatggtgga gaaaatcaaa tcaaaatttg
    50821 gacactggga aaagaaaaat ttgagtatag acataagtga ggctaagttt aatccttgct
    50881 ttatatcaaa aaataaagat actgaacagg tgaacttagc tatctgccta aaaggtattc
    50941 cttttgaaaa tgatgaagaa gtctactcaa tggcagtggt aaataatatt ttcggtggaa
    51001 gtataagctc aaggcttttt cagaaaataa gggaagaaaa aggtttagta tattcaatat
    51061 actcatcaca gacattatat aggaaatgtg gtgaattagg catatttgca agtatgagta
    51121 cagaaaattt acaagatgta tataatttaa taaagaaaga aattgaaaat ataagagaaa
    51181 actatcttac agaaaaagaa atatctgaaa gcaaggaaca gttaaaagga aattatatat
    51241 tggatttaga aagtactagc agtagaatga tgtctactgg taaatctatg ttacttagca
    51301 aaaaagtaaa aactacagat gaaatacttg aatgtataaa taatgttaat attaattcaa
    51361 taaagaaggt tgttgataag gtattcaata tcgaaaatat tggtacatgt atagttggaa
    51421 gagatgtaga aaagattcta cacttggatt aatattgaaa aaagtaaatc tctggaggta
    51481 taaaatatga atttatctga aatagcaggt aaggaaatag ttaacttggt cacaggagaa
    51541 agattggggg tcattggaga atgtgacctt atactagatg aaactacagg cggtatatta
    51601 gcgcttctaa ttccaaagga aaaaggattt tttgccagaa gaaaagataa gtcattttta
    51661 gaagtaccat ggagaaatgt aaaaaaaata ggtaatgaca tgataattat agaatatgag
    51721 ggatatattt aggaggaaaa aatgagtatt ttagtgcaaa agtttggtgg gacatcagta
    51781 gaatcatatg aaaaaatgaa tgaagtatgt aagatagtaa aagcatacaa aaaaaatgat
    51841 gaagaattac aattagtatt agtagtttca gcaatgggaa gaaaaggagc tccatatgct
    51901 acagatacac ttataaattt gtgtagtgca gtaaatgatg agccaagtaa aagagaactt
    51961 gatttaataa tgtcttgtgg agaaataata tcaggaacaa ttcttgctaa tttattgaat
    52021 gcacagggga tagattctgt tttctttact ggtcagcaag caggtataat tacaagtgat
    52081 gaatattcta atgcaaagat aaagtatata aatcctaaaa aggtaaaaag agctttagat
    52141 gatggtaagg ttgtagttat agctggattc cagggtgtaa cagatgatgg agaaataact
    52201 actcttggaa gaggaggaag cgatacatca gcagttgcta ttggtaaagc tttagaatgt
    52261 gaaacagttg aaatttacac tgatgtggat ggtataatga ctgcagaccc tagagtagaa
    52321 ccaaacgcta aagtattgag ttttatagat tatgaggaag tattccaaat ggctgacaaa
    52381 ggtgcaaaag taatacatcc aagagcagta cagttggcta aaagtggaaa tataacttta
    52441 gccataaaga atacaatgaa tccaactttt gaaggtacaa aaataggttc tttatgtaga
    52501 cacttagaag agaatataga atatgaacaa gaaagagatt ttaaggttgc tgttgcaaat
    52561 aaagatagtg tagcacaagt taagattaaa tcagcagaag aagtgtttac agaagtatta
    52621 aatgagatag aaaagaaact tataactata gatatgataa atttctttat atcagaaaaa
    52681 gcatttgttg tagaagatgc tgatataaaa tcattaaagg aaatactaga taaatttgaa
    52741 ttagattatg aagtaaatag agactgtgct aaggtaacat tgatatgttc aagaattgct
    52801 gatgaaatgt ctggtatcat gtcaaaggta gttagaggat tatcaaaagc aggggtatct
    52861 cttttacaaa catcagactc aaatatgact atatcttgtt tagttagtga agaagatatg
    52921 catacagcag tacatgctat acaccaacaa ttttatttaa aataaactgt ataatacaat
    52981 tattttttaa tcaaatttaa gaatttcata tatatcttaa tatatttgat ttatttggta
    53041 aaactaaatt aaatataatt ttgataggag tttttttatg aaagattata gagaagataa
    53101 tgaaagattt gataaagata attgtagtga agaatctagt tgcgaagaga gagaatgtat
    53161 aaagcaattt ggtaccaatg aaatgccacc tcaacctcct aaagatattc aatgtataac
    53221 tataattgga gaaattgagg gacattttat aggaaatcct caaaaaaaag ctacaaagta
    53281 cgagcatata attccaatgc tatactcaat tgaggaaagc aatgatgtta aaggagtgtt
    53341 agttgtttta aacactgttg gaggagatat tgaagcaggt ttagctattg cagaactttt
    53401 aaacagtaca tctaaaaaag tagttactct tgttctagga ggaagtcata gtataggtgt
    53461 tcctcttgct acagcaggag attactcatt tatagcacca acagcaacca tgattataca
    53521 tccagtgcga actactggtc ttgtgattgg tataaatgag acttttgaat attttaaaaa
    53581 gatgcaagat agaataattc aatttataat aagaacatca aatatcaaaa aagatgtact
    53641 tgaaaaactt atgcatgaaa aagatgaact tgtaagtgat gttggtagtg tattaatagg
    53701 aaaagaagct gtggattatg gtcttataga tgaagttgga ggactaaaag aagcacttaa
    53761 aaaattaaga gagcttataa aagaatctga ggaagaaaaa aataatgatt aaaatttgtt
    53821 taaataataa aatttatgat ataatgatgt aataaagtag gagtgttagt gttaaggcat
    53881 tagcactttt tgctgtgaag gggtaaaact tgctaaaaat aaggagtgat attaatggcc
    53941 acgaaaaaga agaaaaaaaa gaaggtaaat accttaagtt ttaatgcaga atatcataat
    54001 ttaataacaa tatttatagg tgtattttta ttatatagtt taaattctaa ttctatggga
    54061 tggatacctg tgttaatgca aaatcttttt aagggactat ttggagggct ttcaatagca
    54121 ataccattta tagtaattat aacaggactt ttaggatttt ttgatggaaa tgaatatata
    54181 tataggctaa gaaagactaa actatactac ataattattt tatttatatt tgtattttat
    54241 ggattattaa atgcaggaac tcttcctgta gatagcccac taaaaggtaa catgtttgat
    54301 gatgttatga aattgggtgt aagtggacaa ggtagtggtt tgatagcaac aactattgcg
    54361 tattatatga gtaaaatatt tggtatagca ggtggatggt taataagtat cttcgcactt
    54421 atactttctg taatgtttat atttaatatt tctataaaag atttggtgtc aaatgcaaaa
    54481 tcaaaatcta aagcatctaa agacagcaat ttaacgttta aagataaaat tgcaaatatg
    54541 aaaaaatctg caatagatat gatgactgat gaagtagatg atactactat aaataaaaaa
    54601 ccaggtttct ttaaagggtt aatgtcaaaa ggtcttaata atgatgatga agaagatgaa
    54661 tattttgaag aagaaaatag tgatggtgta gatgataaaa ctataaaaat agttggattt
    54721 aataaggcag aagatgagta tttagaaata ttagaaggaa ctcaaagtat gccagagtta
    54781 gatgttttaa aagaattaca aaaagctacc aatgaaaatc cagttgtaga tactaaacca
    54841 gaaaaaaaag ttgatatagc taaatctaat ttaaatatag aaaaaactca acctatgagt
    54901 attgtagcag agccagttaa tgaggattat tcaaactata aaaagcctag cattgagctt
    54961 ttaaataaag taaataagaa gtctgatgaa aatggtaaaa agaaagtgtt gaaaaatgct
    55021 tctcttttag aaaagacact atcagatttt ggagtggaag caaaaattaa tcaagttaca
    55081 gttggaccta ctataacaag atatgaaata caaccaagtc caggagtcaa ggtaagtaaa
    55141 attgtaaatt taactgatga tatagcttta agtttagcag ctaaaagtat aagaattgaa
    55201 gccccaattc ctggtaaaag tgcaattgga atagaagttc caaatgaaga agctcaaatg
    55261 gttggtgtga gagaagttct agaaagtgaa gaatttaata attttaactc tcctcttgca
    55321 atgggacttg gaaaagacgt agcaggaaaa attataattg gagatatagg taagatgcca
    55381 cacttactaa tagctggttc tacaggttca ggaaagagtg tgtgtgtaaa tacattaata
    55441 agtagtattt tatataaggc aaatcctgat gaagtaaaat tactattaat agacccaaaa
    55501 gtagttgaac ttgcaaatta taatggaata ccacatctat tgataccagt agttactgac
    55561 cctaaaaaag ctgcaaatgc tttaaattgg gcagttacag agatgaatag aaggtataaa
    55621 ttatttgcag atgctcaagt aaaagatgta acaagttata atgagaaagc ggaagaaaaa
    55681 cttcctaaaa tagtaataat aatagatgag ttagcagatt taatgatggc aagtgcaaat
    55741 gatgtagaag attatatttg tagattagct caaatggcaa gagctgctgg aatgcacttg
    55801 attgtagcaa ctcaaagacc atctgttgac gttataacag gggtaataaa ggctaatata
    55861 ccatctagaa tagcatttgc agtatcatct caaactgatt caagaacaat ccttgatatg
    55921 ggtggtgcag agaagcttct aggtaaaggt gatatgttat tctatccact tggtgcagca
    55981 aaaccagtta gacttcaagg ggcattcata tcagagagtg agtcggaaaa agtaattgat
    56041 tttgtaaaaa gtcaggttaa agatggaata aaatatgaag aagatataat tgaaactata
    56101 tctaaggtta atacttcaaa gagtagtgat gaggatgagt ttttgagtga agcgatagaa
    56161 tttgtcgtgg aaagtggtca agcatctgcc tctatgctac agagaagatt taaaatagga
    56221 ttcaatagag cagctagact tatagattct atggaagaaa gaggcataat tggagcaagt
    56281 gagggaagta aacctagaaa ggtattaata agcaaacagg atttgcagaa tttagaaggt
    56341 gaatagaatt gtcagttata aaaatagaaa aagcacttaa gctagataaa gtaatatttg
    56401 tagatgtgag aactgaagga gaatatgaag aagaccatat attgaatgca atcaatatgc
    56461 ctctttttaa aaataatgaa cataatgaag ttggaacaat ttataagatg cagggaaaac
    56521 atgaagcaat acaaaaaggg tttgactatg tatcgtataa attaaaggat atatatttac
    56581 aagcagcaga acttgcatta aattatgata atattgtcat atattgtgca agaggcggaa
    56641 tgagaagtgg aagtatagta aatttattat catcattagg tgtaaatgta tatcaacttg
    56701 aaggtggata taaggcttat agaaattttg tattagagta tctaaggcat gtaatggata
    56761 caaaaaactt tgtatctgtt catggattga ctggtacagg aaagactgat ttgttacatt
    56821 tattagatga aaaaggaata gatgtactgg atttagaagg catagctaaa aacagtggtt
    56881 ctacctttgg ttttataact tttgataaaa agccaccatc tcaaaagaac tttgaaacaa
    56941 aactatttga aagtgtattt ttttcgaaag agaattatat atttgtcgag agtgaaagca
    57001 aacgagttgg aagtgttaca gttccaaatg aaattttcga ggcaatgaca cgagaaggac
    57061 atcatatatt attagaatgt gatattgaaa atagagttga tagactttgt agagattata
    57121 tttatgataa agatgaaggt aatatattga ttttaaaaga atgtataaac aagtttagaa
    57181 aaagattggg taataaagta gtagatgatt atattgattt attagaatca ggtgaatata
    57241 aagaacttgt aaaaagatat cttttagaat actacgaccc actttatatg cattcagtag
    57301 agaaatataa gtatagtaag attataaatt ttaatgatac acaaaaagct ttagaagatg
    57361 ttatagactt atatgagagt atattggaag gagaaagtga atgttaaaaa tagcattgga
    57421 atcattagga tgctcaaaaa atttagtaga tgcagaaata atgatgggaa ttttaaataa
    57481 caaaggctat aaattaattg gagattttga agaagctgat gtaattatag taaatacatg
    57541 tgggtttata gagtcagcta aacaagaatc tatagatacg ataataaact tttcagagtt
    57601 aaaaaaaact ggcaatttaa agttgttgat agtaacaggg tgtctagccc aaagatattc
    57661 tgaagaatta aaaactgaaa ttcctgaaat agatgctata gtaggaacag gaagctatca
    57721 aaatatagat aaaatactta aagaattaag tgaaatgcat caaattgtaa gccttaatga
    57781 tatagagttt gtatttaatg aagatttacc aagatatata tctacaccta gctatatggc
    57841 ctatttaaaa attggagaag gatgctccaa taattgtact tattgtataa taccaaaact
    57901 aagaggtaaa tatagaagta gaaaatttga agatataatt aaagaagcta agaaattagc
    57961 tgaatctgga gtaaaagaac ttgtggttat agcacaagat acgacaaagt atggttttga
    58021 tttatatgga aaagaaagat tatcagaact tttagaagaa cttgcaaaaa tagatggatt
    58081 taagtggata agggttatgt attcatatcc agaatctata acagaggaat tgatacaagt
    58141 tataaagaaa tatgataata tatgtagtta ctttgatatg ccaatacagc atgcaagtaa
    58201 caatatttta aaattaatga atagaaaaac tactaaagaa gatatattaa ataaaatcaa
    58261 tctgataaga agtaatatac cagatgctat acttagaact acgataatag tagggtttcc
    58321 tggtgaaaca gaagatgact tcaaacaact tgtggacttt gttgaagagg ttaaatttga
    58381 tagacttggt gcatttgcat attcaagaga agaagataca ccagcagata gattgccaaa
    58441 ccatatagat gaagaagtaa aaatacaaag aagagatact ttaatgatga ttcaacaaaa
    58501 aatatctgaa gaacttaatg acaaaaagat aggaaaaact tatgaggtgt taatagaaga
    58561 acagatagaa gataatgtat atacaggaag aacacaaggt gatgcggaag aaattgacag
    58621 tatagtgtat gtaaaaagtg tagacaatct tgaagtagga gaatttgtaa gtgttcaaat
    58681 aaatgatgca atggaatacg atttaatggg agatgtttta tatgaacttg cctaacaagt
    58741 taactttgtt tagaatattt ttaataccag tatttgtttt aattatgttg ctcaatgtac
    58801 caaataaatt tttaatagct tgtattattt ttataatagc ttcaattact gatgctttag
    58861 atggaaaaat agctagaaaa tacaatctag tcactgactt tggaaaattt atggacccat
    58921 tagcagataa gttattggtt atttcagcac ttacatgtat gatagaagac catcttgtaa
    58981 gtagttggat ggtaattata atagtggcta gagagcttac agttagcata ttaagagcta
    59041 tagctgctgc tgatggaaaa gtaattgctg ctggaaacag tggaaaactt aaaacgataa
    59101 ctcaaatggt atctatagtg tttttattgt taggcgctca gtttgaaaat gtattgatat
    59161 taaatattgg tgaaattctc attcttatag ctacactact tacattatat tctggatggg
    59221 aatatctata taaaaataaa gagcttttta tgtcttctaa gtaaaaaatt atgctttaat
    59281 aaataaaaac taatgattaa taaataaaaa ataacaactt aaaattttaa aaatgtaaaa
    59341 aagggtatac tattatagta tacctctttt cattttaaga atattttctt ttgtgtatat
    59401 aattattaat aatggtttat aaataaaatg taatgaataa aaatataatc attagtagcg
    59461 tttttaaatt gactaggttt gatttatgtt atataattaa tatataacaa gagaacaaat
    59521 gtttgtagat aaatgaaggg atggtataga gatgagtgta gatcaagaaa aattaaaagc
    59581 gttgaatgaa gctttaggta aaattgaaaa agattttggt aaaggttcag taatgaaatt
    59641 gggagaagca acatctatgt ctatagatgt tatatcaaca ggagcgattg gtttagacat
    59701 agctattggt ataggaggtc tacctagagg gagaatagtt gaagtatatg gtccagaatc
    59761 ttctggtaag actactgttg cgcttagttg tgtagcatca gctcaaaaag atggaggaat
    59821 agctgcattt atagatgcag aacatgcact tgacccagta tatgcaaaag ctttgggtgt
    59881 ggatgttgat aacctaataa tatctcaacc agatacaggt gaacaggctt tagagatagc
    59941 agaggcattg ataagaagtg gagcgataga tataatagta atagactcag tagcagcatt
    60001 agttccaaag gctgaaatag atggagatat gggtgattct catgtaggtt tacaagctag
    60061 acttatgtca caagcactta gaaagttaac tggttcaatt aaaaaatcaa attgtgttgc
    60121 tatatttata aaccagttaa gagagaaagt aggaataatg tttggaaacc cagaaactac
    60181 tactggagga cgtgcactaa aattctattc atcagttaga ttggatgtta gaaaaataga
    60241 tacaataaaa caaggtgata aagttatagg tagtagaact agagttaaag ttgttaaaaa
    60301 caaagtagca ccaccattta agcaagctga atttgatata atgtatggag aagggatttc
    60361 aaaaattgga gaccttttag atatagctgc tgatgtagat atagttaaaa aatcaggttc
    60421 atggtatagt tacaatgata ctaaacttgg acaaggaaga gaaaatgtta aaaaattctt
    60481 ggaagataat ttagatttaa ctactgaaat agatgagaaa gttagagcat tttacaattt
    60541 aaatgaagaa catgaagaat caggtacttc agtatcaaaa gaaattgtag aagaataagt
    60601 aaaaatacaa aaaaataact agtaattaaa gacatgccac gctccctatt agcttgacac
    60661 aatgttgaaa aaattataaa attaagtgag gataaaatat ctaaatttaa ttttaattat
    60721 taggaatatt ttttaatcat agtgatgaca ttttgggatt attaacgaag gaggtggatg
    60781 tcatagatag tgtagtaatg attgtaatag gtgcagctgt tggtattata gctggatatt
    60841 ttgttagaaa aaatatatct gaagcaaaaa ttggacaagc tgaaaacttg gccaaagaaa
    60901 taatagataa agcacaccac gactcagaaa cagtacaaaa agaaaaatta ctagaagcta
    60961 aagaagagat ccataagtgg agaacagaag ctgaaagaga aaacagagaa agaagaacag
    61021 aagtacaaaa gtatgaaaaa agagtagtac aaaaagaaga agtcttagat agaaaattac
    61081 aaaatttaga atctaaagaa gtaaacctaa gtgaaaaatt aaaggtagtg gaaaagaaag
    61141 aagaagaagt cgaagtaata aaaactcaac agttagaaaa attagaaagt atatcaggaa
    61201 ttacttctga taaggccaaa gaaatcatct taaccaatgc ggagagagat gtaagacgtg
    61261 aaatgtctat aatgataaaa gagatagaaa gtcaagctaa agaagaagct gataagaaat
    61321 caagagaaat tataggatat gcaattcaaa aatgtgcagc agatcacgta gccgaaacta
    61381 ctgtaacagt agtaagcttg cctaatgatg aaatgaaagg tagaataata ggtagggaag
    61441 gtagaaatat aaggacttta gaaaccttaa caggtataga ccttataata gatgacactc
    61501 cagaagccgt aattttatca ggatttgatc caataagaag agaaattgca agaattgctc
    61561 tagaaaaatt aatagcagat ggaagaatac atccagctag aattgaagaa atggttgaaa
    61621 aggcgagaaa agaagttgac aatattataa aagaatatgg tgaacaagct gcatttgaaa
    61681 ctagtgtaca tgggattcat ccggaattaa taagattatt aggtagactt aactatagaa
    61741 ctagttatgg acaaaatgta cttaaacatt ctatagaagt tgctcatata gctggtataa
    61801 tggctgctga aataggagca gatattagat tggctaagag ggcaggttta ttacatgata
    61861 taggtaaggc tgtagaccat gaaatggaag gtacacatgt tgaaataggt atggatttac
    61921 ttagacgtta taaagagtct aaagaagtta tacatgccat gagtactcat catggagatt
    61981 acgaacctca aacagtagag gctgtattag ttacagcagc agatgcaata tcagctgcta
    62041 gacctggtgc gagaagagaa actttagaag catatataaa gagacttgaa aaattagaag
    62101 aaatagctaa ttcatatgaa ggtgtagaaa aatcatttgc tatacaagct ggtagagaaa
    62161 taagaataat ggtaaaacca gaagctataa atgatgaaga aatacatcta ttggctagag
    62221 atatgactaa gaaaatagag gatgaacttg aatatcctgg acaaataaaa gtaagcataa
    62281 taagagaaac aagagctata gagtatgcga agtaaaagaa ataatatcta aataataaga
    62341 aaatattttt agaaatttaa atttgccaca agatatgata atatatcttg tggcttttaa
    62401 attgtttaag tttaatttta tataatgcaa aaataatcta agtgctaaaa gtgctactta
    62461 caaaagatta ataaaataaa gctttatata tgtattattt atataattat ccaagtttgt
    62521 ttgatataat gaagttgtaa aatcaatact ttactacaaa ataaatagta aatactacag
    62581 taaaatctaa aaataggata
//
LOCUS       DAMKPG010000009        61570 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMKPG010000009 DAMKPG010000000
VERSION     DAMKPG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.010"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            243..764
                     /locus_tag="PC621_000655"
     CDS             243..764
                     /locus_tag="PC621_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4107841.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            798..1574
                     /locus_tag="PC621_000656"
     CDS             798..1574
                     /locus_tag="PC621_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4107842.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1917..2870
                     /locus_tag="PC621_000657"
     CDS             1917..2870
                     /locus_tag="PC621_000657"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF4107843.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2888..3907
                     /locus_tag="PC621_000658"
     CDS             2888..3907
                     /locus_tag="PC621_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF4107844.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3938..4189
                     /locus_tag="PC621_000659"
     CDS             3938..4189
                     /locus_tag="PC621_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107845.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4471..6276
                     /gene="lepA"
                     /locus_tag="PC621_000660"
     CDS             4471..6276
                     /gene="lepA"
                     /locus_tag="PC621_000660"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF4107846.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6427..7764
                     /locus_tag="PC621_000661"
     CDS             6427..7764
                     /locus_tag="PC621_000661"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF4107847.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7997..9310
                     /locus_tag="PC621_000662"
     CDS             7997..9310
                     /locus_tag="PC621_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4107848.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            9482..10657
                     /locus_tag="PC621_000663"
     CDS             9482..10657
                     /locus_tag="PC621_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF4107849.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10760..11779
                     /gene="hrcA"
                     /locus_tag="PC621_000664"
     CDS             10760..11779
                     /gene="hrcA"
                     /locus_tag="PC621_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF4107850.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11809..12429
                     /gene="grpE"
                     /locus_tag="PC621_000665"
     CDS             11809..12429
                     /gene="grpE"
                     /locus_tag="PC621_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF4107851.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12521..14368
                     /gene="dnaK"
                     /locus_tag="PC621_000666"
     CDS             12521..14368
                     /gene="dnaK"
                     /locus_tag="PC621_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF4107852.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14579..15733
                     /gene="dnaJ"
                     /locus_tag="PC621_000667"
     CDS             14579..15733
                     /gene="dnaJ"
                     /locus_tag="PC621_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF4107853.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16699..17634
                     /locus_tag="PC621_000668"
     CDS             16699..17634
                     /locus_tag="PC621_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF4107854.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17670..19043
                     /locus_tag="PC621_000669"
     CDS             17670..19043
                     /locus_tag="PC621_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4107855.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19231..19998)
                     /locus_tag="PC621_000670"
     CDS             complement(19231..19998)
                     /locus_tag="PC621_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4107856.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20028..21137)
                     /gene="ugpC"
                     /locus_tag="PC621_000671"
     CDS             complement(20028..21137)
                     /gene="ugpC"
                     /locus_tag="PC621_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF4107857.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            21396..22133
                     /gene="cas6"
                     /locus_tag="PC621_000672"
     CDS             21396..22133
                     /gene="cas6"
                     /locus_tag="PC621_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF4107858.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            22138..23613
                     /locus_tag="PC621_000673"
     CDS             22138..23613
                     /locus_tag="PC621_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107859.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            23633..24646
                     /locus_tag="PC621_000674"
     CDS             23633..24646
                     /locus_tag="PC621_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF4107860.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            24662..25468
                     /gene="cas5"
                     /locus_tag="PC621_000675"
     CDS             24662..25468
                     /gene="cas5"
                     /locus_tag="PC621_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF4107861.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            25495..27903
                     /locus_tag="PC621_000676"
     CDS             25495..27903
                     /locus_tag="PC621_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF4107862.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDDMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEGALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     NYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            28344..29291
                     /gene="prmA"
                     /locus_tag="PC621_000677"
     CDS             28344..29291
                     /gene="prmA"
                     /locus_tag="PC621_000677"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF4107863.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            29310..30068
                     /locus_tag="PC621_000678"
     CDS             29310..30068
                     /locus_tag="PC621_000678"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF4107864.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            30070..31368
                     /gene="mtaB"
                     /locus_tag="PC621_000679"
     CDS             30070..31368
                     /gene="mtaB"
                     /locus_tag="PC621_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF4107865.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            31411..31761
                     /locus_tag="PC621_000680"
     CDS             31411..31761
                     /locus_tag="PC621_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF4107866.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            31924..32103
                     /locus_tag="PC621_000681"
     CDS             31924..32103
                     /locus_tag="PC621_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF4107867.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            32133..32576
                     /locus_tag="PC621_000682"
     CDS             32133..32576
                     /locus_tag="PC621_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF4107868.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            32687..33184
                     /locus_tag="PC621_000683"
     CDS             32687..33184
                     /locus_tag="PC621_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF4107869.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            33377..33940
                     /gene="raiA"
                     /locus_tag="PC621_000684"
     CDS             33377..33940
                     /gene="raiA"
                     /locus_tag="PC621_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF4107870.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            34153..34389
                     /locus_tag="PC621_000685"
     CDS             34153..34389
                     /locus_tag="PC621_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF4107871.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            34402..35745
                     /gene="yqfD"
                     /locus_tag="PC621_000686"
     CDS             34402..35745
                     /gene="yqfD"
                     /locus_tag="PC621_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF4107872.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            35684..36799
                     /locus_tag="PC621_000687"
     CDS             35684..36799
                     /locus_tag="PC621_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF4107873.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            36856..37317
                     /gene="ybeY"
                     /locus_tag="PC621_000688"
     CDS             36856..37317
                     /gene="ybeY"
                     /locus_tag="PC621_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF4107874.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            37323..38042
                     /locus_tag="PC621_000689"
     CDS             37323..38042
                     /locus_tag="PC621_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF4107875.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            38161..38562
                     /locus_tag="PC621_000690"
     CDS             38161..38562
                     /locus_tag="PC621_000690"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF4107876.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            38626..39519
                     /gene="era"
                     /locus_tag="PC621_000691"
     CDS             38626..39519
                     /gene="era"
                     /locus_tag="PC621_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF4107877.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            39531..40910
                     /gene="mgtE"
                     /locus_tag="PC621_000692"
     CDS             39531..40910
                     /gene="mgtE"
                     /locus_tag="PC621_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF4107878.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            40983..41138
                     /locus_tag="PC621_000693"
     CDS             40983..41138
                     /locus_tag="PC621_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF4107879.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            41104..41877
                     /gene="recO"
                     /locus_tag="PC621_000694"
     CDS             41104..41877
                     /gene="recO"
                     /locus_tag="PC621_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF4107880.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            41870..42469
                     /locus_tag="PC621_000695"
     CDS             41870..42469
                     /locus_tag="PC621_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF4107881.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            42629..43507
                     /gene="glyQ"
                     /locus_tag="PC621_000696"
     CDS             42629..43507
                     /gene="glyQ"
                     /locus_tag="PC621_000696"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF4107882.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            43507..45573
                     /locus_tag="PC621_000697"
     CDS             43507..45573
                     /locus_tag="PC621_000697"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF4107883.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(45739..47286)
                     /locus_tag="PC621_000698"
     CDS             complement(45739..47286)
                     /locus_tag="PC621_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF4107884.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            47566..48333
                     /locus_tag="PC621_000699"
     CDS             47566..48333
                     /locus_tag="PC621_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF4107885.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            48616..48813
                     /locus_tag="PC621_000700"
     CDS             48616..48813
                     /locus_tag="PC621_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4107886.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            48842..49918
                     /gene="vorB"
                     /locus_tag="PC621_000701"
     CDS             48842..49918
                     /gene="vorB"
                     /locus_tag="PC621_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF4107887.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            49921..50640
                     /locus_tag="PC621_000702"
     CDS             49921..50640
                     /locus_tag="PC621_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF4107888.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            50653..51192
                     /locus_tag="PC621_000703"
     CDS             50653..51192
                     /locus_tag="PC621_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF4107889.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            51349..52407
                     /gene="buk"
                     /locus_tag="PC621_000704"
     CDS             51349..52407
                     /gene="buk"
                     /locus_tag="PC621_000704"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF4107890.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            52425..53318
                     /locus_tag="PC621_000705"
     CDS             52425..53318
                     /locus_tag="PC621_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF4107891.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            53348..54556
                     /locus_tag="PC621_000706"
     CDS             53348..54556
                     /locus_tag="PC621_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF4107892.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            54580..55842
                     /locus_tag="PC621_000707"
     CDS             54580..55842
                     /locus_tag="PC621_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4107893.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            56418..57881
                     /locus_tag="PC621_000708"
     CDS             56418..57881
                     /locus_tag="PC621_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF4107894.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            57937..59073
                     /locus_tag="PC621_000709"
     CDS             57937..59073
                     /locus_tag="PC621_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4107895.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            59309..60424
                     /locus_tag="PC621_000710"
     CDS             59309..60424
                     /locus_tag="PC621_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF4107896.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            60576..61445
                     /locus_tag="PC621_000711"
     CDS             60576..61445
                     /locus_tag="PC621_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4107897.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        24443 a         6531 c        11230 g        19366 t
ORIGIN      
        1 atatgcttaa ttaagataat ttttccaata atcaagataa tttttttcat tagtatgtat
       61 gatagatgca tcaaacaata taactaatat gttagaaggt tatatgtaat taatctcagt
      121 gtttatatag aatagcattt gtagcattta ttatagttgt aattactact tatatcatag
      181 acttgaatag tctaaaaaaa tagactataa tatttttata gactaaagaa agggctgata
      241 agatggacat agcaaaacta attttaaatc ctgcaaggct tcgtatctta caatatattc
      301 gacttcatgg cagtgctcgc acatctgata ttgtaaagta tttaaatgat attcctcgtg
      361 caacagttta tcaccatgta aaaattttag aagagaacaa tatgattgaa gtaattaaag
      421 aaaatcgtgt gcgtggtact attgaaaagg tttatgcact aaaggaatat acaacatcta
      481 tggaaggaga gaccttcgtt gcactttcta cagcatttta tgtaggtctg atgcaggaaa
      541 tgaatgaata ctttagcaga aaagagcaag accataaaaa ggataatgta tttttctctt
      601 cagcattact atatatgagt gataatgaat atgaaaatct tcttaaaagt atagctgatt
      661 tattgaaacc atatatagaa caaaagccta aatcaaatct aaaacttaga aaactttcaa
      721 ttatatcttc tccacctgtg gttgataatg ataaaacaaa ataacatgaa tcttattttt
      781 ataaaaggag gttgtatatg aaatttcatg aatttggaga taaaaacaaa ccacatatta
      841 tgttgattca tgggggaggg aatgcgtggt ggaactattt gcgacaagca gaagttttgt
      901 ctaacaacta tcatgtgata cttcctacat tggatggtca tggagaagaa tatgaaacaa
      961 catacatttc tacagaggat acagcagata agttgatatc atatatagat gaaaattgca
     1021 gtggacatct tttcgctttg tgtggggtat cacttggtgg tcaaattgtc atggaacttt
     1081 tatctcgaaa acctaatatt actaaaaaag caattattga tggtagtatt tgctacccaa
     1141 gacctcttat ggcaaggttt tgtattgctg gtattcgatt ttttggagga cttttgttta
     1201 gtgagaaggc ttgtcgcttt caaattgcta tgatgccaaa attgttacct gaaaatatgc
     1261 aatatccaga tgaaataaaa gcgtattata tgacagatat gccacatgta agaaaagaaa
     1321 cattctataa tatgtatcgt acttatatga tgaactatac acttaaagag aatgttaaaa
     1381 aaaccacagc acaagtaatg tactggtatg gagaaaaaga aatgaagtgc gttaaaaaat
     1441 ctgcaaaaat gtttcagtct tatgtaccct cttgtgagat ttatgaagct aagggctata
     1501 atcatgggta tttatcgctt tatcttccaa atgaatggtt ggaaattgca gaaccatttt
     1561 tccaaaagga ataaaggtgt taggtgatta ttgtataatt attgctgttt ttttatatag
     1621 taaataaatt aggtataaat ataaaaaagt tattttagtt aatatattag tgaaagctta
     1681 ataggaaaat tataaataga ttaaaggtag cgcctatata aatagttgct accttttttt
     1741 gcttgtaaat aaatgataga taagaaagtt tactgacaaa tcactgacta aacccaattt
     1801 tcttacattg aaagccttaa ttttacttgt tttaaataaa tttacaattt cttgttataa
     1861 aatactaaat tttgttaaca attataatta gcatttaatt tatactggag gtttatatga
     1921 ttaatgtaag aacagattta gctttagaag ctagtgaaat gtgtgaaaag tctcaagaag
     1981 gaagtagtat tcctggtgta aaaatagaaa ctaaagaatt agaaaattgc attgtaacta
     2041 aagttgaagt aatagacagg caaggcgcag agataatgaa taaagatata ggaaagtata
     2101 taactctgga aagtaatctt atgaagtttg atgatgatga gtcgagagaa gaaatgataa
     2161 gttatttaaa agaggagtta gtagatatat ttggccaaga caaaaataaa aaaaccctgg
     2221 taattggtct tggaaataga aatattactt ctgatgcgtt aggaccaaag agtgtatcaa
     2281 aaacacttgt aactagacat ctttttaaaa attataataa agactatgat gatgatttca
     2341 ctgaggtatc agctttaagc ccaggagtta tgggtgttac tggaatagaa actagtgaaa
     2401 tagtaaagtc attggttgaa aaagtaaaac cagatagagt agtagcaata gacgcattgg
     2461 cttcaagaaa aatggaaaga gtaaactcta ctatacagat atcaacagca ggaatatcac
     2521 ctggtggagg tgttggcaat actagaaaat ctctaactaa agaaacttta ggcgtggatg
     2581 ttattgcaat aggcgttcca acggtggttg atgcagcaac tcttacaata gatgtcttag
     2641 atatggcaat agacaattta atagctcaat cagaagaaac agaaagtttt tatgaaatgt
     2701 tgaaaaaact taaagaagaa gaaaaatatc atctaataaa agattcgtta gacccatatg
     2761 acaagaactt aatagtaaca ccaaaggata tagatgatac aatcgaaaat ctatctataa
     2821 taataagtga aggcttaaat aggtctttac atccaggtag attggtataa aataaggagg
     2881 agcaattatg tttaaaaaat gcattaaagt agtaaccttg acttttatat tagcatgcat
     2941 tttaccagga aaatcattag ctttgaatca ggatgatttt ttaaagtttt tagtaaattc
     3001 atcttatcca gaagctaaag tcgaaggaaa tgatacagaa aataaaaaga ataataaaaa
     3061 taaagaaaca agcaaagaaa ataaggaaga aagtaaagaa gaaaacacta aatctaaaga
     3121 tgcaagtaaa gttgataata aaaaagagag tgaaaaagaa tatataaaac tttatgttgg
     3181 taaagaaaat gttccagata ttgagtcaaa aaattcagac acaactgaaa ctaacacaac
     3241 atctagttca gattataagg atgatttaag agtaaccaaa gaaaatccaa gaattttgat
     3301 atatcatact catggatgtg agacttattc aaattcacct gatggaaatt atcattcaag
     3361 agataagaaa aattcagtaa tggaagtagg aagtgcatta actagtgctt tagatagcaa
     3421 aggatggggt gtagtccata ctactaaata ccatgactat ccatcttata ataactctta
     3481 tgcaagtagc ttaaaaacaa tacaaagtat attacctaaa tataactcag tggatatagc
     3541 gatagactta catagagatg ctagggattt aactaatcca gccactaagg aaaaagacca
     3601 tttaaaatat acaactatga taaatggaga gagagtatca aagttcttct ttgttgtagg
     3661 aggtaaaaat acaaatagaa aacaacttag agctttagca gaagatataa ctgcttttgc
     3721 tgagaaaaag tatcctggtc tagtatctcc aatagtagaa aaagactatg ctagattcaa
     3781 tcagtttgca gttaagaatc atatgttagt agaaataggg aataacgcaa caagtgtaga
     3841 agaatctaag gctactacaa agtatttagc cgagatttta gatgaatatt ttaaacaaaa
     3901 aaattagagc tttaagttat aattaggagc atgctaaatg agccgattag aaaaaaatat
     3961 agaaagtaaa agtaaactaa gaaaatacaa gtatattatg agaatcttat ttttgattac
     4021 tatgattata ggtacatcag tttgtgtatt tatagtagac agtaatgcaa aaagtatgct
     4081 aggaatgaaa caatatgatt ttccaagtat taaatttgat ataacaaaat ttgattttga
     4141 cataccaaat tttgatatac atgaaatcat aaaaaaattt aataagtagt aataagtgaa
     4201 taaggtttat tgattaaaat aagccttatt ttagttatat atttttaatg tataaaataa
     4261 aaattcatta actgtatttt ataggattta ctttaagttt acgatattat ataatattta
     4321 gtgttagact tgataatcca catataatgt ggataatttt gtttagtgcc aattagttta
     4381 tattcaaaaa ttttaaattt ggtatataaa gagtttttag ataatgtaaa ataataacta
     4441 taagaataat caagaggagg gaattttaag gtggacaata aacaaagtag aactagaaat
     4501 tttagtataa ttgcacatat agaccatgga aagtctacct tagctgatag attaatacaa
     4561 caaactgggc ttgttagtga aagagacatg aaaagtcaat tacttgacaa tatggacctt
     4621 gaaagagaaa gaggaatcac tataaagctt caaaacataa gattaatgta taaagcgaag
     4681 gatggaaatg aatattattt aaatcttata gatacacctg gacatgtaga ctttaactat
     4741 gaggtatcaa gaagtttagc tgcatgtgaa ggagcactat tagtagttga tgcagctcaa
     4801 ggtgttgagg cacaaacttt agccaatgtt tatcttgcta tagaccaaga tttagaaata
     4861 ttaccaataa taaataaaat agatttacca agtgcaagac cagaggaagt aaaaaatgaa
     4921 atagaagatt taatagggct tgattcaagt gaagctcctc ttatttcagc taaaactggt
     4981 ttaaacatag aagatgtttt agaagatatt gtaaaaaatg ttcctccacc taagggagat
     5041 aatgaagctc cattaaaggc attaatattc gattcttact atgatgctta caaaggtgtt
     5101 gtagcttatg ttagagtatt tgaaggaact gttaaaaagg gtatgacaat aaaaatgatg
     5161 aataccaata aaaagtttga ggtaactgaa gttggtgtta tggctccagg tcaaactgaa
     5221 cttagtgaat tatcagcagg tgatgttggt tatattgctg ctagtataaa agatataaga
     5281 agttgtcgtg ttggtgatac aataacagat tcaaataatc ctacagaaga acctttacca
     5341 ggatacaaaa aagctactcc aatggtatat tgtggtatat atccaggaga aggagaaaag
     5401 tatgagaatg ttagagatgc tcttgaaaag cttcaagtaa atgatgctgc acttgaatat
     5461 gaagctgaga cttcagcagc actaggattt ggttttagat gtggtttctt aggtctttta
     5521 catatggaga taatgcaaga aagacttgag agagaattta accttgatat aattactaca
     5581 gctccatctg ttatatatag agttacaaaa atggatggag aggttgtaat gatacaaaat
     5641 cctgcaaact taccagaacc atctgagata aagatgatag aagagcctat agtaaaagga
     5701 gatataatag ttcctaaaga ctatgtgggt gttgttatgg agctttgcca ggaaagacgt
     5761 ggaaatatgt taaatatgga atatatagat gagagaagag taatgcttca ctatgacctt
     5821 cctctcaatg aagttgttta cgatttcttt gatgctttaa aatcgagaac tagaggttat
     5881 ggttctcttg attatgaggt taaaggttat gtagcatcta cacttgtaaa acttgatata
     5941 ttaatcaata aagaacaagt ggatgcactt agttttattg tccatgagac acgtgctttc
     6001 cctagaggaa aggcaatgtg tgagaaatta aaaggagaaa ttccaagaca tcagtttgct
     6061 atacctatac aagctgctgt tggtaataag gttattgcaa gagaaacaat aagcgctcta
     6121 agaaaagatg ttcttgctaa atgttatggt ggggatatat ctcgtaagaa gaaacttctt
     6181 gaaaagcaaa aagaaggtaa gaagcgtatg agacaaattg gttctgttga agttcctcaa
     6241 aaagcattta tgtctgtact taaactagat gaataaattc ttataaattt agataaatat
     6301 atttaaaagc tagagtgatt ttagaattat ttctaaatta atctagcttt tttgtggtat
     6361 attttaagta aactcataga gaatataagt aagaatatat taaagaagaa taaaaaggtg
     6421 atatttatga ttaatataga aattcctaaa aaagtagact atataattaa agaattagaa
     6481 acgaatggtt atgaagctta tgtagttgga ggatgcgtta gagattgttt attagaaaga
     6541 gttccaaacg attgggatat aacaactagt gctagaccag aagtggtagt agaattattt
     6601 gaaaaaacaa ttccaactgg tatacaacat gggactgtaa cagttatgat tgaacatgaa
     6661 ccatttgaag tgacaactta tagaattgat ggaaattata gtgatggtag acatccagat
     6721 tcaatagaat ttacaaataa catagttaag gacttgtcaa gaagagattt tactatcaat
     6781 tcaattgcat acaattcaaa gacaggatta gtcgacccat ttaatggcta tgaagatata
     6841 caaaataagt atatacgttg tgttggaaat ccagtagata gatttgaaga agatgcactt
     6901 agaatgctta gggcagttag attttcagca caacttaatt ttaaaatagc tgaaggtact
     6961 aagcaaagta ttcataaaaa agcagatttg ataaaaaatg tttctatcga aagaatccag
     7021 acagagttta ataaaatatt agtatctgat tcatctaaat taaacttatt aaaatcaaca
     7081 ggtttgttga aatttataat tcctgaaata tgtgaattag aagatgttac tcaacacaat
     7141 ccatatcaca tctatgatgt acaaaaacat actttaatat ctacagaagt aattgaagat
     7201 gaactgtatt taaaacttac aatgttgttt catgatttag gtaaaaaagt aactaaaaca
     7261 actgataaaa atggtgttga ccatttttat actcatagta gagaatctgt aaagattgca
     7321 aagaagattt tgagaagact taagtatgat aattatacta ttaataaagt tttaatatta
     7381 atacaatatc atgattatag aatagaacct aaaaggaaga taattaaaaa gttattaaat
     7441 aaacttgaag atgttgaatt atttgaagat ttaataaaag ttaattgggc tgatacactt
     7501 gccaaaaacc caaagtatgc aaaacaaaag atattaaatc ttattgagtg tgaaaaagaa
     7561 tttaaacata taataaatca aaaggagtgc tttaatctta aagatttagc tattaatgga
     7621 aaagatttga tatctatagg tataaaacca ggtaaagata taggacatat tttaaataag
     7681 atgcttgaaa tagtcataaa taatccagaa ttaaatgaaa aagaaatact aaaagaaaaa
     7741 gcattaaata tatatacatt ttagattcaa atataaaaaa tttaattact ttaaaaaatt
     7801 atagaagaaa atacttattt agataaaata aataattttt agagaatatt ttagaagtta
     7861 cgattgtatg atttttaaac gcatagcatt tactctagga ctatagacta atataaaaaa
     7921 aaactttggg aatactaaaa ccataaattt ataaaattaa tattttatat actgtaaaac
     7981 agtgaggagg gacagtatgc atactcaact acaaaagact attggtcttt cagcggctct
     8041 ttctactgtt gtaggtatgg ttataggttc gggagttttt tttaagccac aagcaatata
     8101 cactacaact aatggagctc caggtctagg tattatagcg tggcttttag gtggttttat
     8161 aacaataact gctgggctta cagctacaga aatttcagct gctataccaa aaactggtgg
     8221 tatgatgatt tatatcgaag aaatatatgg agaaaagtta ggctttttaa ctggatggat
     8281 gcaaactgta ttatttttcc caggtacatc agcagcatta ggagtaatat ttgctcaaca
     8341 agcatctgaa cttttaggaa tgagcccaaa taatatggca aatgtacttc ctatagcaat
     8401 tggagttata ctatttttag cgttattaaa tataataggt tcatccttag gaggaaaagt
     8461 tcagacagta gccactattg gaaagatgat acctttaatt ttaataatag tctttggatt
     8521 tattaaaggt caaagtagtg aagtattaaa tccatttgta ggagatggtg taaatgcatc
     8581 aaatgcactt ggacaagtat taatagctac tttgtttgca tatgatggtt ggataaatgt
     8641 tggagctata tcaggagaaa tgaagtctcc agagaaagat ttaccaagag caatagttgg
     8701 tggtttatca ttagttatgg cagtttatat tataataaat atagcttatc tttgggtagt
     8761 accagcaagt gaattagcaa cagttacttc cccagctaca ttagtagcaa caagattgtt
     8821 tggaaatata ggtggaaaag taataactgt gggtatatta atatcagtat ttgggacatt
     8881 gaatggatat ttattaacag gttcaaggat accttataca ttagctgaaa tgggaacttt
     8941 accagcatct aaaactcttt taaaagtaaa ttctggaggt tctcctgtaa attcaatact
     9001 acttataact gtgttagcct gtgtatatgc attatctggt caatttaatc ttttgacaga
     9061 cttaactata ttttcaatat gggtatttta tgtattgact tttataggtg ttatgaggct
     9121 tagaagacaa aagccagatt tacacagacc atataaagtc cctttatatc caataatacc
     9181 aattatagct atactaggtg gactgtttgt aataataaat caaatattga cttcaacaat
     9241 tatatcttta ggtggaatat ttataacact attaggttta ccagtttatt attatatgaa
     9301 aaaaagataa ctacttaatt taagttagat aaatttgggt aaaggtgaaa attaactttt
     9361 atccaaattt tttatactgt tatacaatat aagcattata tattacttga tgtgttattt
     9421 tgtttgattt ataatttagt aattgatgct ttaagataat ttaagaaatg aggaataaaa
     9481 aatgctagga ctttatgtac atattccatt ttgtgtgaaa aaatgtaagt attgtgattt
     9541 taattcttat aaaatggaca tagattcaaa aaaaagatat atagaagact taaaaataga
     9601 aatgaaatta tatagtaata aattatataa agataataaa tataaaaata aagaatgctg
     9661 tagtctaaat aaaaatgaca aaataacgag tatatttgta gggggaggta cacctagtat
     9721 actaacatct gatgaaataa gagaagtatt tataagtata aaagaaatgt ttgatataga
     9781 tgagaatgca gaaataacta tagagtgtaa tcctgggaca ttaacattag aaaaattaaa
     9841 aactatgaaa gagattggca taaatagact tagtataggg ctacaggcca ttcaagaaaa
     9901 gcatttgaat tttataggaa gaatacatac ttatgaagaa tttgagaaaa actataaaga
     9961 tgcattaagt gtaggtttta aaaatattaa tgtagattta atgtacagtc ttccaaatca
    10021 aactctatgt gactggaagg aaactcttga aaaagttgta cacttaaatc caacacatat
    10081 atcagcgtat tcccttatac tagaagaagg aactgaacta tataatatgt atgaaagtaa
    10141 taaatttgag cttatagatg aaaatgtaga tatagaaatg tatgagtata caataaacta
    10201 cttaaagtca aaaggatata atcaatatga aatatcaaat tactcaaaag aaggctataa
    10261 ttgtgagcat aatattttat attgggaatg tgaacattat atagggattg gagcaggagc
    10321 ttctggctat attaatgaga acagatacaa taatgtagaa tctttagaag attatcattt
    10381 aagtttggta aaaagagaaa aaccaataca agaaaatgaa attctttctg agaaagatat
    10441 gatagaagaa aagatattta tggggcttag aatgaataaa ggcataaaat ttgaagattt
    10501 taaaaaaaaa tttggaatag attttagaga gaagtataat aagcaaatag aaatgctatt
    10561 agctagaaag cttataaatc agagctttga aggtatacaa ttaactcaaa aaggaagaga
    10621 aatatctaat agtgtattta tagaatttat ggaataattt ttaaggtgtt gacaaaataa
    10681 atttataatg gtatataata tatataatct ttttagcact cgaacttagt gagtgctaac
    10741 agctaggagg tataataata tggaattaaa tgaaagaaaa ttaaatatcc tcaaagctat
    10801 agttaaagat tatatagaaa cagctgaagc tataggttct agaacaatat ctaaaagaca
    10861 tgatttaggt gttagtgctg ctaccataag aaatgaaatg gctgaccttg aagaattagg
    10921 gtatttaatt cagcctcata cttctgcggg tagagttcca tctgaaaaag gctataagtt
    10981 atatgtaaac tctcttatga gtaaaagcga attggatgat aatgataaga ttcttattga
    11041 acaatgtatg aatcataata taaatcatat aaaagaattg attcatgaaa cctctaagct
    11101 attatcgcaa ctaacaaact atacaactgt agcagttact aagagtttaa taaatcaaag
    11161 tgttataaag cacatacagt tggttgctat gaatgacaat aatattttac ttatagttgt
    11221 aacagataaa ggggacttaa aaaaagctaa ccttacaact aatgtatatt tagatcaatc
    11281 taagttaaat ttgatttctg ataatcttac aaggaagctt ttaggtaaaa gtataacaga
    11341 tttggatgat aatctaattg cttttattaa atacgaaata agtgagtatt ctggacttat
    11401 agatgagctt ttaaatgctt tgaactcaaa tatgaaagaa gaagattttt ctctatcatt
    11461 aaatggagct actaacatat ttagttatcc agaatttaat gatgtattaa aagcaaagtc
    11521 atttttaaat atgttggaaa aaaaggaaac aatagctgac ataattaaat caaagggaat
    11581 acaaaaagac aatctaaata taataattgg tagtgataat gattgtgaac tggcacagga
    11641 ttgtagtata gttacagcaa cttataatgt agatagagac ttggttgggc gaataagctt
    11701 tataggccct accagaatgg attatgctag aatttactct ataataaatt atatgagttt
    11761 attaattaat agaaagtaaa gaaattttaa attgcgaggt gtaaagtctt ggataaaaaa
    11821 aatgagcaac aggaagtaag agaagaaaat gatacttcta ttaaccaaga atcggaaact
    11881 caagtagagt tagaagagga agttgttaat gaagaatgtg aaacatcaag tgaaaaaact
    11941 gatgaaaaag aagttgatga tgaaaatgta acagatataa attctaagtt agcagaaaaa
    12001 aaattgcagg atgagttgga tgaattaaat gataaatatc aaagacttca agccgaatat
    12061 gcaaactaca gaagaagaac tcaacaagag aaagagacaa taggcgtatt tgcaaatgaa
    12121 aaaataataa ctgagttgat accagttata gatagtatgg aaagagcctt agatgcatgt
    12181 gaagataagg aagatactat gtataagggt ataagccttg tacataaaca attaatagac
    12241 actttggtaa aatttggagt agaagaaata gaagcagaat ctaaggaatt tgaccctaat
    12301 ctacatctag cagttatgca agaaagtgta gatggagttg aagcaaatca aatagtaatg
    12361 gtacttcaaa aaggatataa gctaggaact aaagttgtaa gaccttcaat ggttaaagtt
    12421 tcttgctaag tataaattaa aggttaaatt atttaaccta atataaaata ttaaaataag
    12481 taaaacttaa ttatataatt actcgaggag gattttagtc atgggaaaaa taataggaat
    12541 agatttagga acaacaaatt catgtgttgc agtgttagaa ggtggagaag cacaaataat
    12601 agcaaatagt gaaggtatga gaactactcc atcagtagta gcatttacaa aagatggtga
    12661 gagaatagtt ggagaacctg caaaaagaca agcagttaca aatgcagata aaacaataac
    12721 ttctataaaa actcatatgg gaactgatta taaagtaaat atagatggaa aatcatatac
    12781 tccacaagaa atatcagcaa taatattaca aaaattaaaa tcagatgcag aaagttactt
    12841 aggacaaaca gtaacagaag cagttataac agtaccagct tactttacag atgctcaaag
    12901 acaagcgact aaagatgctg gtagaatagc agggcttgat gttaagagaa taataaatga
    12961 gccaacagca gcagctcttg cttatggtat ggataaatta gaccaagaaa agaaaatatt
    13021 agtatttgac ttaggtggag gaacttttga cgtatctata cttgaaatag gagatggaac
    13081 ttttgaagtt ttagctacag ctggtaacaa tagattaggt ggagatgact ttgaccaaat
    13141 agtaatagat tatttagcag atgaatttaa aaaagctgaa ggtgtagatt taagaaatga
    13201 taaaatggct cttcaaagat taaaagaagc agcagaaaag gctaagaaag aattatcatc
    13261 aacaatgagt tcaaacataa acttaccttt cataactgct acagcagaag gaccaaaaca
    13321 cttaaatata gatttatcaa gagctaaatt tgaagaatta actagaggtt tagttgaaaa
    13381 aactatggaa ccaactaaga cagctttaca agatgcagga ctttctacag gtgatataga
    13441 tgatgtatta ttagtaggtg gttctacaag aataccagca gtacaagaag ctgttaagaa
    13501 gtttatagga aaagaacctc ataaaggtat aaacccagat gagtgtgttg cagcaggtgc
    13561 ttctatacaa gctggagttt tagcagggga tgttaaagat ttattattac tagatgttac
    13621 tccattatct cttggtatag aaactatggg taatgtaatg actaagataa tagaaagaaa
    13681 tactacaata ccaactaaga agtctcaaat attctcaact gctgctgata accaaacagc
    13741 tgttgatata catgttcttc aaggtgaaag aagtatggct tatgacaata caactttagg
    13801 aagattccaa ttaacagata taccaccagc acaaagagga atacctcaaa tagaagttac
    13861 ttttgatata gatgcaaatg gtatagttaa tgtttcagct aaagatttag gtacaggaaa
    13921 agagcaaaaa ataactataa catcaaacac taatttatca gaagcagaaa tagagcaaaa
    13981 gataaaagaa gcagaaatga atgcagaagc tgacaaacaa aagaaagaaa aaatagaagc
    14041 attcaatcaa gctgaatcta ctatatatca aactgaaaaa actttaaatg aacttggaga
    14101 taaaataagt tcaggtgaaa aagaggatat agaaaaagct atagcagact taaaagctgt
    14161 aaaagataat caagatgcta cagcagaaga acttaagaaa gctacagatg aagtaatgac
    14221 taagttccaa aaagtatctc aagaaatgta ccaaaaagca gctcaagaac aacaagcggc
    14281 acaaggtgca gaacaagcac aagacaatgg acctaaagat gacaatgtag tagatgctga
    14341 ctttaaagaa gtagatgaag ataaataagc tatataatat gtaacactaa atgataattg
    14401 gaacatatat gatttgtgaa aatcatatag atgttaaaga tatatggaca atacacatca
    14461 taaagaatat aatatatcat agattatata acttaaagta gaatataaga attaatgata
    14521 gcttgtagtt tattattcta caagctatcg ttttgtaaaa tgaaggtggt gtcaattttt
    14581 gagtacaaaa agggattatt atgaagtctt aggtatcagt aaagatgcag aagctcaaga
    14641 gataaaaaaa gcttatagaa aattagcaat gaagtaccat ccagatagaa atcctggaga
    14701 taaagaggcg gaagaaaaat ttaaagagat aaatgaagct tatgaagttc tatctgatga
    14761 tacaaagaga aaaacttatg accagtttgg acatgatgga ttaaatggtc aaggtggttt
    14821 tggtggtcaa ggaggatttg gcggtcaagg ctttggtggt tttgaagata tgttcggaga
    14881 tatatttggt gacatgtttg gaggaagctt cggaggtgga agagcaagaa gaagagggcc
    14941 tcaacgtggt gcagatataa gacagagtgt tactatatct tttgaagaag ctgcatttgg
    15001 taaaaagatg tctataaaag taaatagaag tgaagagtgt gaagaatgta atggaactgg
    15061 agcaaaacca ggaacatcaa agaaaacttg ttcaacttgt aatggaactg gccaagttag
    15121 aactgttcaa agaactccat ttggtaatat agcaagttca agaccatgta gtgcctgtaa
    15181 tggaactgga gaagtgattg aatctccatg tagtaaatgt catggaactg gaaatacaag
    15241 aaaagttaag actatagaag tagatatacc tgctggtata gatgatggtc aaatgataaa
    15301 actttctggt caaggtgaag taggagaaaa aggtgctcca agaggagact tatatattgt
    15361 agttaatgtt aagtcacatc cactatttac tagagatgga aatgatattt attttgaaat
    15421 gccaattaca tttgttcaag caactttagg tgatgagata gaagtaccta ctttggatgg
    15481 aaaagttaaa tatagtgtgc cagaggggac acaaactgga actgtattta gacttaaaga
    15541 gaaaggaatc cctagaatta gaggtaattc aagaggagac caatatgtta aggttgtagt
    15601 tgaaattcct aaaaaattaa acgataagca aaaagaacta ttgagagaat ttgcgaagga
    15661 gtgtgggtcg aacgtccacg aaaagaagaa aaccttcgga caaaagatag aagatatgtt
    15721 taagaaaaag tagttaaagt agccaatata taatcatatt tttgaaatct tagttattta
    15781 taagattgtc aaaggagata ttatgatttt tatattggct atttttgtat aaaaaataaa
    15841 tgtgcataaa ataaaactga atataaataa ttttctgaaa aatacttgtc ctaaaatata
    15901 taatgtgcta taatataatc aaataaaaga aaggggtgat tccagtggga acggatgtgg
    15961 cactgccaaa tgatgaagaa aactttattg taatatacaa aataaaatta gatactttta
    16021 gtgctagatt aagtactgga aaaactcatg agctgggata attattgtaa ttacctagac
    16081 tcaaactttt agtagtaaaa tagtaatatt atatagacaa ggtatctaat ttttaaatct
    16141 tttgtatgag ttaaaatttt tataaagggt atatctgatt agaatgtaat tagggaaacc
    16201 tagttgtgaa ttacttttag tatatacatg tgtaaagaaa tatgtatatg gtattggtta
    16261 ttgtgctaga tgtgtatgta aaggaatatg tgtctagttg ggtatatgta aagaaatata
    16321 cttataatct atgaaaaatt tttagtataa attgctaaaa cattgagatt gttttttata
    16381 gaaagttagt ttataccaac taaaaatgtg cttaagattt gtttgatgtt taattataaa
    16441 taaggttgta gataaaaaaa taagtaaaga caaaggttta aaatacttgc tttaaatata
    16501 gtttgttttg aacacttgcc aaattaaaat aattaataaa atagacatct tctcatagta
    16561 aaatatactg tgagaggatg tttttgcttt gttcataaac ttttatgaaa aatttgcata
    16621 gtgtcacatt ttaatatact atatatataa taaaatatag ataaaattaa atgattcaaa
    16681 agagaaaggt gaaaagagat gtattatata ggtgtagaca taggaggaac aggtatacaa
    16741 gcaggagtag ttgataacta tgggaagata atttttaggt cagagtgtaa aacagtaatt
    16801 gaaaaaggct ttgaaggaat acttaatgac ataaaaataa tgatttataa gttgttggaa
    16861 gataataaac ttactatgag tgacataaaa tctattggat ttggtgttcc aggttttata
    16921 aataaagaag gattagtaac ttgtgtaaat ttaaaatgga ataaaaaagc ttttaataaa
    16981 gagttaaaaa gaagatttcc agatgctgaa attcatggag aaaatgatgc aacagtagca
    17041 gcacttggag aagcaaagtt tggaagtatg aaaggtgcta atgttggagt attatatact
    17101 ttaggaactg gtgtaggtgg aggaatagta attaatcaaa aagtcttttc tggagcacat
    17161 ggattgggtt cagaaatagg tcatcaaatt ataggagaaa actattttaa ttgtaattgt
    17221 ggaaataatg gttgtgtaga aactttttgt tcagcaactg caattataaa gtattctcaa
    17281 aaactgatag aagaaggaga aaagagtagg attttagatt tggcagaggg aaatttagaa
    17341 aatgtaaatg caaagatggt gtttgatgca tatagagaaa atgatttagt ggcaattaaa
    17401 gtaataaata gatttaaaga ttatttagca aaaacttttg caaacacaat aaattcttta
    17461 gacccagaaa ttatatctat aggaggaggc atttctaaat ctagcgatat aatacttgat
    17521 ggaatagaag atttggtaag aaaatttgta ttatacaaaa cagaagatat agctacaatt
    17581 acatgtgcaa ctttaggttc agacgcagga attataggag cagctttttt ataatgtaaa
    17641 tgaactgctt attggaggcg tgatgatata tgggtaaaac aaatgctaga agaacgcttt
    17701 ttttgatagc aataggttca ggaactatgc tgaatcctct taattcttct atgatttcac
    17761 ttgctttaca tagtatacaa aatgattttc atattagttt tacaactgta tcttggattg
    17821 tttcagcatt ttacctagct agtgctattg cacaaccagt ttcaggtaag ataggagatt
    17881 taataggtag aaaggtctta tttttgagtg gtttgatgct tgtaatttta tcagcatcca
    17941 tagtaccact tgttcaatca ttctttatat tgatttttat aagagtaatt caagcaattg
    18001 gaacaagtac cctatatcca tctggagttg ctcttataca aaataatata aaagaaagac
    18061 aatcttctgc attagcagtt ctaaccattt ttgcttcaac tacagcagga ttaggtccga
    18121 cacttggagg attattgttg gacttaggag gatggcatgc tatatttctt gtaaatatac
    18181 ctgttgtttt ggtttctata tgcttagggt attttttatt cccaaaagaa gtaaaagaaa
    18241 agaaatctat aaaagaaact ttaaaaaatg ttttgtcaag attagatatt attggcatac
    18301 ttctattttc aattgcaatg atttttatat tactattttt gctatctatg aaggaatctt
    18361 ttaacttaga acaacttgta tttggtataa tactgatgtg tctttttatt tggcatgaat
    18421 tgagaacaaa attgccattt atagatatta agctttttgt aagtaatcct aaattgtcaa
    18481 aagtttactt gcaatttatt atattaaatt tctttaatta tattttgttt tttggtcttc
    18541 ctagttattt tcaagatgcc ttacattata gtgcaaagtc gactggatta tttatgctat
    18601 ttatgtctgg tataggtata tttatatctc cattaactgg aaaatggata gataaatctg
    18661 gaactagatt tcctcttgtt acatcatcaa tctttatgtt tattagtgca gtacttcttt
    18721 cagtagtttt tatccatata cctgtaatag gaaagggtat tatattatca ttagcaggta
    18781 ttagttatgg agttggtaat gtagccctgc aatcctctat gtttgaagaa agtcctcgtg
    18841 acagtatagg gacagcttct ggtctgtttc aaactagtcg ttatttaggt tctattttag
    18901 caactgtttt acttggaatg gtatttgaag gaaatataac atcagaacaa tttcagatat
    18961 taggttatgt tatggtggtc ttaggtacaa taagtttttt attaaatttt atattaaaga
    19021 aagaattaag aaaagtagaa tagtggattt atggatttaa taagtctaat aactgataag
    19081 tttaaaaata ttgtttgtaa aacataactg gtttgatatt tattaattct aagttttgaa
    19141 gcatagagta ctcgataagt atacaatatt ttgaattaaa attaagcaga aatttgaact
    19201 agtctttcaa atctctgctt aatttagtag ttaaattttt ttatagagaa aataagattt
    19261 gacacttaga taaaaaatca ttgcattttc aaaattcttt aaatcaaacc caagtatctc
    19321 attaatcttt tccaatctat atactagcgt gtttctatgt aaaaacatac tagtagatgt
    19381 cttagaaata tttaaatcat tatttataaa ttttattcct gtatatagaa gttcttcgtc
    19441 aatagattta atcttattaa aatctattaa tttattgagt atattatcct cattaattaa
    19501 atctaaagtt ttatatctaa taaggtcaaa caaatttaaa aacttatttt cagcattgtc
    19561 ttttataata tgaggtagta aatcagaaca taactgtaat ctaaaataaa aatcagcttt
    19621 actatctaat tcaccaagat aaaaaatata agtactttga taagtttcag tttcaattat
    19681 attttgtgta gttacattaa tatctaaatt atatttagaa tttactataa aaaaaccatt
    19741 gtgttgtatt atatttatat cttcaaataa attataaaaa atatttttta ggtattcaaa
    19801 gtctatatga gattggtaaa ttaaataaaa gttgcctttt aaattatcaa gattattttt
    19861 atctagaaaa tcaataacat aaatagcatt attgaaagta actaaatggc tactagcatc
    19921 taacttttgg ttttcatagg gatagatatt tattttttta ttaagctgtc tttctaaaaa
    19981 atcttttaac tcttccataa agtctcctta aaaataagat tatattctta tcttatagct
    20041 aaagtagttt ctttgtcaaa tatgtgtact ctactattgt caactgaaag tgttaagtca
    20101 tcaccaacat taattctgtt tttactgtca aatctaattg ttatcgtatt ttcgcctagt
    20161 tttaagtgag cataagtttc tgctcccata agttctttta tttcaattct tgatttaaaa
    20221 caagtatctg gattagattt aataaaagta tcttctacat gaatatcttc tggtctgata
    20281 cctataacaa cttcattatt aatataattg tcttttatta aatcatagtg tttttcttta
    20341 ttcaatttaa gcttaatatg ttcattttgt gcataaattt tatcattttc ttctattaat
    20401 attacatcaa taaaattcat ctgtggtgct ccaataaatc ctgcaacaaa tatattagct
    20461 ggataatcgt atacatcttg tggagttgca atctgctgaa ctactccatc cttcattact
    20521 acgattctat cagccatagt catagcttca acttggtcgt gtgttacata gataaatgta
    20581 gttccaagct gttggtgtag cttagttatc tctgttctca tagcagttct aagttttgca
    20641 tctaagttgg atagaggttc atccattaag aataccttag gatttctaac tattgcacgt
    20701 cctaaggcaa ccctttgtct ttgtcctcca gataatgttt taggtttttt atttaaaaga
    20761 ggtagtaagt ctaatatttt agcagcttct ttaacttttt tgtctatttc atctttaggc
    20821 aattttctaa gtttaagtgc aaatgccatg ttttcgtaaa cagataagtg gggatataaa
    20881 gcatagtttt gaaaaaccat agctatatct ctatcttttg gttctatatc attgacaagt
    20941 ttatctccaa tgtatagttc tccttcagag atttcttcaa gaccagcaat cattctaagt
    21001 gtagtcgatt taccacaacc agatggtcct actaaaacga taaattcttt atctttaatg
    21061 tctatattta tattttttac tgcgttaaat ccatttgagt aaagttttga tatatttttt
    21121 aaaattactt ctgacataat attctccttg atatttaaat tttaatgata tagtatcaaa
    21181 tttttttatt atattaaatg tgtaaaatga ctaaatgtat ttgtttaata tgtatattta
    21241 atttttagca aagaataaaa ctaacagtaa aatatattta aaaaattaaa aaacaaaatt
    21301 aaatttatta tcaaaaaatc attttatgat atgttttatt ttttatatta caaatagaga
    21361 ataatgaatt atactagaaa gtaggaggtg aaaatatgag cagaatttca atttttatgg
    21421 agtcatgtaa tggcaaattt ccaaatgaaa attcaatgtt gtcagtttcc tttataaaaa
    21481 atattttaaa tgcagaaaac aaagattttg ctaaaaatat atttaactat ggggaagaaa
    21541 gaaaaaacaa taagcaaata aaggatataa atacagccat ctacatacca aatcttataa
    21601 gaagcaaaaa tgagttgctt gttgatggag atataatatt caatataagt ttttataatt
    21661 attctatgtt cagtaaatta tacaatggaa ttttaaaagt aaaggagcta gaatataaag
    21721 gttatagatt taaaataaaa aatataaaaa tacataaaga acacgagata aaagaaaatg
    21781 gtgttatatt taaaactatg tctcctataa tagtaaagaa taaagaaggt aaatatttag
    21841 atgtagaaga ttcaaattat atagaatgtt taaactatat agctaatcta actctggaga
    21901 gtataagagg tagtgggctt agaaagccat tggaatttat acctcttaac ttcaaaaaaa
    21961 gagtgttaaa agaaaagatt cgtggattta aagtgagaga atattactat ataaatgctt
    22021 atgcaggaac atttttttta aaaggaaata tggaagattt aaatgcactg tataaaatgg
    22081 gaattggata tagaaggacg gaaaatgcag gaatggttga tatattaaag tagcgaggtg
    22141 agagaaataa aagctaaatt ttatagatca ggtgatattg taagagatat tggcattgtt
    22201 tacttttatg aattactaat ggatttaaaa aacgaactag aaaaaaatga ttataagcta
    22261 aaatttactt gtgagttaaa tagaaattat ttatctttag aatctgcaga atatatagac
    22321 cctaagtata tatctgatta tatattagaa aatcaacttt ttaaggtatt taaaaatggg
    22381 aaaaaggaaa ccttagaaaa aatatttagt aatatagata atttaacgta tgtaaatttt
    22441 attgaagaat tagataaatc ttctgcaact gagaaacaaa aggaagggat taaaaaagat
    22501 tttaaaaaca gaagatttcc atatgttaga aatagtggta agtatggatt aaacacgagt
    22561 ttagaaaata tggaaacaaa tgtaaaaaga atagtagata ctgtaattaa atcaaatata
    22621 gagatagatg aaatagataa acttgatttg acacaatatg aagaaaaaga tagtgtatgt
    22681 aatgtttgta atataaataa aacaacaaaa ctagatattg atttaagaga gcgaaaggac
    22741 tctaagtata actttttatt tagaggtgca gaaaaatcag gctttaaaag aagtggacaa
    22801 gttgaaagta atatttgttt tgagtgtgaa tttttcaact taatgtgtct tttatatata
    22861 aatttaaaaa gaccaatggt ttttgcatat acgaatgatt taagggaatt agcattttta
    22921 aatcataaga taatgcttaa aagaaagatg tattcagata aatcatttta taaaaaactt
    22981 ttgcatgaaa aaataagttc gattagacta tataggtttg atattgatac gaataaaggt
    23041 ataattctaa agtttgactc tattatagag tataaagaat tattaaaaaa aattgaactt
    23101 atagatatta tagataatta taatttttca agagagccag ggaatactag aaatttgggt
    23161 aaaaaaatga ttgataatgg gaatttatca aatttaaaag aattattact tgataatata
    23221 agtgttttaa ggcaactaac tggaacttct cgagaaatgg attttgtatc tagctcatat
    23281 aatatcggtt tatatataaa attgtgttgg attgtagatg gaggggagga gtataaaatg
    23341 aaaaactata tggaaagtaa tttaatatat tcaagagtag gtaaagactt atttaataaa
    23401 atgacagatg aatccagaag aaaaaatttt tctatgaaag tgattcaact acttaagtct
    23461 aatgatagaa ctcagctatt tcaaactata atgcatgtgt tagtttctaa tggaatctta
    23521 ataggagaag ggtttgtaga aggtctaatg cagagttctg aattagaatt aaatacgaat
    23581 gtaggtctat ttattcaaga aatcatgaaa taaaaaaggg ggaaaattta aaatgaatga
    23641 aaaaagagta aaaggagtaa cattaactat actgacagaa agcccagttc cactttcata
    23701 tgaccaaggg tatggaaatt atacaccaat aaaaaaagaa caatatagag aaaaaataca
    23761 tgcaaaaact agtatagcca caataacata tgatttaaga agaatgctcc accaagaata
    23821 tggttggaat ttaagtaata ttatttttgg gaaaaaagga aatatatatc catctattaa
    23881 gcaaaatgta ggatgtgtag atgaaaatgg attagagact gatgtatttg gttatttgat
    23941 accacttaaa gataaaggtt ctataagtaa ggcttctccg cttagaataa taccttttag
    24001 gtcattaaat tcatatagag gttcaacaca gttgataaca aatagaggtt ttttaagtag
    24061 cgaatttggt agaaaatatt atgatgagaa ggaagaaaat gaagtactta gagatgaaaa
    24121 ctttccaaca tcacaagcat tagcaataga agagacttta tcagattact acgtttatac
    24181 aataacattg gagcttgata gaataggagt agtagaagta gaagatggaa agttgctttt
    24241 accagaagaa agaaaattta tgagtaagga gttaagagaa aaagcagtaa aagatatatt
    24301 agatgcaatt actgtgttta caagaaacat aaaacattca tcagtactgt taaaaccact
    24361 tgcagtaatg ggtggagctt ttgataaagt tgtaccgttt ttttgggatg atgtagatta
    24421 taatgctgat agtggtgaga taaatttaga gggagtcata gaaactatag aaagttatag
    24481 ccttaaagaa agtaacacaa tacttgctat caatgacaaa ttaaaaattt ctaataaaaa
    24541 ggaattaaat aagtttaatt taagtaaata ccctgtaaaa gagattaaaa atctagcaga
    24601 tagattggaa attggagaag ataatatgtg gtatttaaaa gagtaggagg ttttttgaat
    24661 tatgaaagta ttacaatgta aacttaagca accaactgct cactatagag acccaaaagt
    24721 atttcaaaat gaatatataa gtactttaaa tttaccatct aagaccacaa taatgggaat
    24781 gataacgtat ttatgtgata gaagattaaa ctctgatata gatataggaa taattggaac
    24841 acatcatcat agagaactag aattttcaag aggagagaac attgattttt ggaatgaata
    24901 tacaaatatg agaaaaggca aagataaaga aaagtttttg cttcaaggaa actactatga
    24961 ttactataaa gaacataaag ctcaaaatag catattaaac tatgaagttc ttaaagaagt
    25021 agaactgact atatttattt cttgcaagga tgatgaagaa cttgaattta ttaaaaaaaa
    25081 gcttgaaagt ccatgtaaat atgcaaattt aggaagaaaa gaagattttg taataccaag
    25141 tgaaaaaggg tgttttgtga aagaagttga gttaaaagag gttatgccta tgaatactcg
    25201 tgatgctata aaagaaaata taaaattaaa gaatacatat gtaagagttg acttaagaga
    25261 taaagagaat gtcgagacta taataaatca aggtgtattg attgcattac cttataaata
    25321 taaagattta gaagcaaata gagatgatag agtttatgag ttttgccatt atatatatgt
    25381 tgataatgat ggtatatatc ctaaaaatat taaggtaaat gtatgtaatg tatgtattga
    25441 tacaaaagaa gtttttacat ggctttaaat tattttgaaa gataggtgtt tatcatgaaa
    25501 aatatcatgc aaaaagaaat attagctaag tcatataatt taagaactaa tcaacaagaa
    25561 caaacattag ttgaacatat taaggattta tttgaagtat tagaatctat attagaacta
    25621 aatctatatt cagataaaga tgtagagata ctaaaaatat gttgtgcttt gcacgattta
    25681 ggaaaaatta attcaattat gcaacaaaaa atggagatta ataataaaat atcgtatagt
    25741 tgctcagaag aagagaggaa aaagttagag tctgacaaaa aatctttaaa aaagatagca
    25801 agacacaata tattttctgg agcattttta aaagatatat tagaaaaaat gaacttgtta
    25861 gaagaagata agttatatat atataaaagt ataatgttac atcatggaaa ctatgaagat
    25921 tatatgaggt taagtactag taaagttcaa gaagaaattt atgattatat tgaaaaaggc
    25981 attttagaaa aagaagagtt taatctgaaa gatatagaaa gttatataaa tgacatttta
    26041 aatgtagatt ttaaatttgg tgaagatgta ttggattatg attacataga taaattaagt
    26101 gaaagtatgt ttatagacag tgattataac aatggtcaaa tagatgattc agttttaaat
    26161 tatagaaagt ttaagtatat tttatataaa gggatgctga acttaataga ccatagtgca
    26221 tcaagtagac agaaaggtat aaaattttat aacgatttta ctgatgaaga aattgatgat
    26281 atgattttaa atgagatata taagagtcag ggtaatttag aaaaaaataa tatagagttc
    26341 aatactatac aaaaaaggtt gagagaactt tctggaagaa atgttctgac tgttgcattt
    26401 acaggttcag gaaaaacagc tgcagattat agaaagactt ttaaaagaaa attcttttta
    26461 gtgccaaata aaatatcagc agaaagtttt tatagaaaaa atatttttca aaataaaaat
    26521 gatttagata caagaagtag taatgattac attggattac ttcatggaga tataaactta
    26581 tattcagaga atgaagataa tggagaacat gattttgtat tgacattaag ggatatagat
    26641 ttgagtataa atttttgtaa accttgtgta attgcaacag ttgaccagtt attacttagt
    26701 atgtttaaat ttccgggata tgaaaaaata ttcgcagccg taaaaaatgc atctataact
    26761 gttgatgaag tacatttatt ggaaccaaag atgtttttga ttttgatata ttttatgcag
    26821 tttgcatgca agtacttaga tgttgatttt cacttaatga cagctacact tccaaaatca
    26881 tataaagagc aaatgataaa caagggaatt atttttcaag aagaaagtaa tgaaaatgtt
    26941 actgataaag gagaaatagt ttttattgag agcaacaagg aagaagaaat ttgtgaaggt
    27001 aaagatgtaa aggtttcttt tataaaggaa aaagaaatca agtcaatagt tgaaggtgct
    27061 ttagaaaata aacaaaaaat tcttataata aagaatacta ttgattcagt caatagaaca
    27121 tatgagtttt taaaagaaaa tttatctgat aagtatggtg gtgtagatat agatgtttta
    27181 catagtagat ttaagttcaa agataaaaaa gagaaatata gtaaaatatt aaatggagag
    27241 ggtgatattt ggatatcaac tcaatcagta gaaatatcct tggatttgga tttcaatata
    27301 ataataagtg atttagcaac tatggatagc ttaatacaaa gaatgggtag atgcaataga
    27361 aataataaat atgaatatgg aaacttttat attctaccta gtgaagataa aatttatgat
    27421 aacaaactta agaacacaac taaaaacatc ttaaaggaca tattaaaaac taactctatt
    27481 tttacaatgg ggattagaaa aactattttg gatgattact atgataatag tgtagttaaa
    27541 aactattttg aagagaattt tataagttgt gataaagaaa ttaagaatat ttatggtata
    27601 aataaagaaa tttttgatgg attggattta atttttaatt ttgaacctta caaaaatatt
    27661 gttgatagta agaaggaagc agctaaaatt tttagagatg ttgatgttag ttacaaaatt
    27721 attctggaag aagattttta taaggaagat agggagttac aacaagattc gattcaagta
    27781 tcagggttta tatttaatag attatattat tatggattaa taagcaaagt agaaggatac
    27841 atggtactaa aatctagtag taagtttgag tataatagta ctgtaggctt aattttgaag
    27901 tgagcatatg tttaaatata atataccttt agacattgaa aatacaaaaa gtactactgt
    27961 aagcccactg taaaaactat tttttaaaag gaaaaataag ttgtgagata gagtgtctac
    28021 aagtgtatat agataataaa tttgaggttt aagattaact aagtgggttt caggtacatc
    28081 gcgaaaataa ttataagttg gagattttgt atgtttaaga ttaactaagt ggatttcaaa
    28141 tattgatata gtagatataa tagaagtttt attattaggt ttaaaattaa ctaatcggat
    28201 ttttaaatat tctgactatt atataaggat attattagct tgaatttaaa accaataata
    28261 taaaacaaca aaatattata tttatgatat aataaaagtt atggaaaaac aggaatatta
    28321 ctatagagaa gagaggtaaa cttatgaata attggataga agtaactata aaaacaacaa
    28381 cagaagcagt tgaacctata acgaatatac tatatgaaca aggagctgga ggagctgtaa
    28441 tagaagaccc aaaagacttt ttattccaaa agaaaaatga actagactgg gattatgttg
    28501 aagaagaagt atttaaaaag aatgaagaag atgatgttct tataaaaact tatgtttctg
    28561 aagaaaaaaa tgtgatggaa tttgtcgaaa taataaagca aaaagtttta ggattaaaag
    28621 attttggtat agatataggt gaaggaagtg tatcattgga ccaagtaaat gaagctgatt
    28681 gggcaaatgc atggaaagca tactacaaac caacaaaagt aggacaaagg gtagtagtaa
    28741 agcctacatg ggaagattat gctatgcaag atggggattt aatcatagag cttgaccctg
    28801 gaatggcatt tggtacaggg acacatgaaa ctactagtat gtgcattaga gaattagaaa
    28861 agtatgtgaa taaagattct aaagtatttg atattggatg tggtagtgga atccttgcaa
    28921 tagcagcagc taaattagga gcaaaagaag ttgtagctgt agatttagat gaagtggctg
    28981 taaaagttgc aaaagaaaat gttcttgaaa ataaagttga aaaaagtgta tctgtaatgc
    29041 atggtaattt aactgatgta ataaaagata aagcggatgt aatagttgca aatataatag
    29101 ctgatataat aaagatactt gcaaaagatg ttcaaaactt tatgaaagaa gatgctatat
    29161 ttatatcttc aggtataatt ttggataaag tagaagaagt taaagaaagc ttaattgaaa
    29221 atggatttga aatagttgaa gttcaaaaat taggtgaatg gtcagcaata gtttctaagc
    29281 ttaaaaaata agggatatgg tgacgtataa tggatagatt ttttgttgaa aaaaataata
    29341 taaatctaca agataaaact tgcactatag aaggtgaaga tgtaaaacat atttcaaaag
    29401 ttttaagatg caaactaggt gagaaacttg aaatatgtga caagaataat aatgagtata
    29461 tttgtgagat aatgaatata gataagagta tagttaattt agagatttta gaaaaagttg
    29521 atataaatag agaatcagaa cttaaagtaa ggttatacca gggacttcca aaagctccaa
    29581 aaatggaaat gatacttcaa aaattaactg aagtgggtgt agaagaaata atattagttc
    29641 aaactaagag aagtgttgtt aaagttgatg acaaaaaaga agataaaaaa tttgaacgtt
    29701 gggaaagaat aatttatgaa gcagcaaagc aaagcaaaag aggtaagata ccaaaattaa
    29761 gaggtgtttt aagttttaaa gaagctttag aagatatgaa aaaaaataat gtaaatattt
    29821 gcccttatga aaatgaaaga acagtttcaa taaaacatgc actaaaaaaa tgtgattcta
    29881 atatagatag tgttggaatt tttataggtc cagaaggtgg attttctgaa gaagaaatag
    29941 agcaaataca aaaaaataat tgtaatgttg tatcattggg gcctagaata ttaagaactg
    30001 agacagcttc tgttgtagca tcaacaatag ctttgtatga actaagtgat ttaggaggag
    30061 aaaaataaat tgaaaaaagt agctttttat acacttgggt gtaaagttaa tcaatatgaa
    30121 acagaagcta tgctagaact atttgagaaa gatggttatg aacaagtcaa tagtgaagag
    30181 tatgctgatg tttatgttat aaatacatgt acagttactc atatgagtga tagaaaatct
    30241 cgtcaatata taagacgagt taaaaagaag aatccagatg caataatagc cgttgtagga
    30301 tgttattctc aggtatctcc agaagaaatt ttggatatag aagaagtaaa tttagtcatg
    30361 ggtacaaatg atagaagaaa gattgttgaa gaaattaaaa agataaattc aagtaagaaa
    30421 gttagtacag ttgatgacat aatgaaagtt aaagcttttg aagaaataga aatcagccaa
    30481 actaatggaa agactagagc atttatgaaa atacaagatg gatgtgacag attttgtact
    30541 tattgtatca ttccttatgc ccgaggacgc gttagaagta gagatattga tagtatagtc
    30601 gatgaagtga aaaaattggc taataatggc tataaagagg ttgtattaac aggaatacat
    30661 gttgcctctt atggaaaaga tttaaaggat agggatatta aacttcttga tgtaataaaa
    30721 caaataaacc agatagaaaa aatagaaaga attaggctta gttctgttga accaatttta
    30781 tttacagatg aatttgtaaa tgaagtttta aagatggata aagtttgtcc tcattaccat
    30841 ttatcacttc aaagtggatg tgatgaaact ctaaaaagaa tgaatagacg atacacaact
    30901 ttagagtaca aaactattgt agatagattg agaagtaaaa tgccagatgt ggcaataact
    30961 acagatgtaa tagtaggttt tcctggagag acaaatgaag agtttaaaaa aacgtacgag
    31021 tttttaaaag aaatagagct ttcacaaatg catattttta aatattcacc aagaaaagga
    31081 actccagctg caactatgga aaatcaagtt gaccctcaaa tgaaacactt tagaagtgaa
    31141 caacttttaa atttaagtaa agtaaatttt aataaatttg caactaaatt tatcggccgt
    31201 gagctggatg ttttgtttga acaaaatata gaaggcaata aatatgaggg attaacttca
    31261 aattatataa gagttgttgt tgaaagtgac aaaaatatac aaggtcagat attaaaagtg
    31321 aaaataaatg atgtaaaaga tgaatatgta gaaggaattt tgctataaca tgtagaaatt
    31381 ataatattta taagatagga ggtggaaaga atggattgta ttttttgtaa aatagcgaac
    31441 ggagaaatac cttcaactaa agtttatgaa gatgatagag tcttagcttt taatgacttg
    31501 aacccagttg ctccatatca tatattagtt gtacctaaaa aacattatga tagtttaatt
    31561 gatattcctg ataaagagat ggacattgtg tcacatattc atgttgttat taataaaata
    31621 gctaaagaga aaggttttga ccaaactgga ttcagagtca taaataattg tggaagtgat
    31681 ggagggcaag aagttaaaca tttgcactat catattttag ctggtaaaaa gcttcctaat
    31741 tacgaagcgg gacaaaacta aactaaatta ttgaaaaatt gagttccttc ttgataaaaa
    31801 tacttgataa aagtaaaaat ttagtttata ataaacaaag tgttaggttg taatgcatat
    31861 tatgcaaaaa gaacatagtt acagtcccag cataattgcg ctattggagg gagggaaaaa
    31921 gaaatgtcag aagtaagagt tagagagaat gaaacattag acagcgcttt aagaagattc
    31981 aagcgtcaat gtgcaatgtc tggcatcatg tcagaagtga gaaaaagaga gcactatgat
    32041 aagccaagcg ttaagcgtaa gaaaaaagct gaagcagcaa gaagaaaaaa cgctaagaaa
    32101 tagtagtaaa taagaataaa gaggtgaagg ggatgtccct taagcaaaag ttacaagagg
    32161 atttgaaatc ttcgatgaaa aacaaagata cagtgagaaa atctgtagta actttaataa
    32221 gagcttctat aaagcagtat gaggttgata atagggtcga gcttgatgaa gatggaatta
    32281 tcgatgtaat agcaaaacaa ttgaagcaac gtagagatgc attagttgaa tttgaaaaag
    32341 ctggaagaga agacttaata aaagaaacag aaggtgaaat tgaagtttta aaagagtacc
    32401 tacctcaaca attaagcgaa gaagagttag aagaaattgt aaaatctact atatccgaag
    32461 taggggcaac ttctatgaag gatatgggaa aaataatgtc agttatccaa ccaaaagtta
    32521 aaggaagagc tgacggaaaa cttataaata agttggtaaa acaaaactta caatagattg
    32581 atatagccta gagagaaatc tctaggcttt ttatagtttt tgggaataac gagtcatatt
    32641 tgagcatatc tataatcaaa gaaaattttg aaagaagggg aaaaatttga ttatatcagt
    32701 aaaagagatt aggaattttt tgataaacat atttatacca ctaataatag gttacttaag
    32761 tagcatctta acaatgatta tcagtggaac agatatttca acctattatt tacaactcac
    32821 aaagccaagc tttgcaccac cagcttttat ctttcctata gtttggacta tattgtacat
    32881 attgatgggt atatcttcat acctaatatt aagaaagggt tacaacttac ctaaggtaag
    32941 agatgcaatt ttttattatg gattacaact tgtattaaac tttatttgga gtatactatt
    33001 ttttggattt ggacttagat ttacagcttt gatagatata ataataatga ttttggtttc
    33061 tataataatg atttcgaagt tttctaaaat agataagaga tctggataca taaatttgat
    33121 ttatttaatg tggcttgttt atgcaggatt tttgaactat tttatatggt ttattaataa
    33181 atagaatgta aagattttgt aaattaccct gtacttttaa taatatcata acaaacgaaa
    33241 aataaataga ctattaaaaa taatcatttt catatacaat aatattaatt taaatacaat
    33301 aaatttataa aaattgtatt taaagattaa catattaaag tattagaatt ggaaaggctt
    33361 gaagggagag gtttttatgc aaataatagt atctgggaga caaatgaaat taactgacgg
    33421 aataaaagga tacgtagatg gaaaattaag taggttagaa aaataccttg atcctgaatc
    33481 agaagtgaaa gtaacagtaa gtgcgaaaaa ggatagacaa aaagtggagg ttacaataat
    33541 tcctattaac ggtcaaataa taagagcaga agatgtagaa gatgacttat atgcagcaat
    33601 tgatattgtt tgtgacaaat taagtagaca agttgtcaaa tataaaacta aagttaaaga
    33661 caaagttcaa aataataaga gcataagatt tgaaaatctt gactttatag ataatagcag
    33721 tgaatttgat gattatgatt atgatgatga agaagatgaa aatatagtaa tagaaagaag
    33781 aaagaaattt aatgtaaaac ctatgagttc tgaggaggcg atacttcaaa tggaactagt
    33841 aggacacaat ttctatatgt ttagaaacca agataatttt gaaataaaca tagtgtataa
    33901 gcgtaaagct ggtggatatg gtttaataga acaagattaa taaaaattgt atacgtttag
    33961 attttatata agaaaaaata aaaaggtaat catgttgatt ttttatcaat atgattacct
    34021 ttttattgtt taaataatat attagatatt aggtatttat tatataaaaa ttttaaaact
    34081 aatctgttac aacatcaata aagtgcataa atacctattt caagcataaa attaattaag
    34141 ggggtaatgc ttatgttaga aatatccact gatttacaag taaatcaacc agttattact
    34201 gttacaagta atacgtttat tagtatagaa aattatctat caatattaga atatgaagtt
    34261 gatttaataa gaataaagac taaagttaaa acaattaaaa tatctgggga taaactttca
    34321 cttaagtata ttactgatag tgagataggt ataaaaggga ttatctacaa tgttgagtat
    34381 gttgattagg aggagataca cttgataagt tttataaggg gatactatgt tatagttgtg
    34441 gaaggagtag gactcgaaca atttttaaat catcttataa gaaatgggat aagtgtttac
    34501 aatgtaacta gaataaaaaa tactaaaatg gagtttcata tagatagaca agatataaaa
    34561 gaatttaaga atgtttatag aggaagtaag tttgatataa aggtaaagca aaaaactggg
    34621 gtccctttta ttatcaaaag agtttataag cacaaaggta tgtggatttg tgctcttgtt
    34681 tctttatttt tattgatgtc tacatcgcag tttgtgacag atgtatatat acaatctcct
    34741 gaaggtataa aaaaagaagc tcttagaaat gagctttata aggttggagt aagaccagga
    34801 gtctataaga agagtattga tagaaaagaa gttagagacc atatgatgtc aaaatttaat
    34861 gatgtggcat atttatctat caatgtaaaa ggaacaaata tatttgttac tgttacaaaa
    34921 aaggcagaat ccctaaaatc tacagaccaa tcaaattatt gtaatgtaat tgctttaaaa
    34981 aatggtataa tagaaaaggt aatccctaga agtggtaaat cagttattaa atctggagac
    35041 atagtccaaa agggagatgt cttattaaat ggagctaata ccaagtcaat accagaagta
    35101 tgggcaagta ctttttatga gtccacaaaa aaggcaagtt atgtagatac tgtaaataaa
    35161 aaaactggag aaaagaaaaa tatatataca ctaagttttt atgataaaga gtttacaatg
    35221 agaaaaaata taaaatataa agattatgtt gttgaaaata aagaaaaaaa actatcaata
    35281 ggaaactata cttttccaat aaagataaaa acgagtactt tttatgaaac aaaaaaggtt
    35341 gaagtaaaaa ggaataaaga agagttaaaa aaagaattat ctgaaaaagc tttaaaagag
    35401 ttagaataca taatcccagc ttcggctaga ataatagatg taaaacataa ctttaaggtt
    35461 aataaaaata tgttagaata tttaataaca gttcaaacta gtgaaaatat tgctaaaatt
    35521 tatcctctta gtaagtcaga agcagaaagg tttataaagg aagaaagtaa accagatgaa
    35581 ggtgaagagg aagttccttc caatccagaa aaaagaccat taaatgatat aagaaatgag
    35641 tttgatgagg ataataagga taataaagac caaaacaatt ctgatgaaaa taatagtaat
    35701 caaaatagta ataatagtca aaataataat agcaataaca actaataaaa ttaaacttaa
    35761 aatcttagga ggactaattc aattgataat acaaaagaaa tttacaatag cagatggtaa
    35821 ctttgaaaga gagttatttg gcaactttga tgaaaatgta aaattaatag agaagacttt
    35881 aaatatagat gtaatactta gagaaggcaa tataatttta attggagaag aaaaaaatgt
    35941 agattctgca ttaaaactta tgaatgaatt acaccaaact gtatcaaatg gtaagcattt
    36001 agataagcag agtatttctt attcattatc tcttttgctt gaaggaagtg aacaaaaaat
    36061 aaaagaactt gaggggacaa tcgtcataac tcaaagggga aaagctgttc aacctaaaac
    36121 tttaggtcaa aaagaatata taaaacttat agaaaataat gatataactt ttggtgttgg
    36181 acctgctgga acaggaaaga cttatcttgc ggttgctatg gcagtaaaag cttttaaaag
    36241 agatgaagtt agtagaatta tacttacaag accagctgtg gaagcaggag agagtctagg
    36301 ttttttacca ggagatttaa aggacaaagt tgacccatat ttaagaccac tatatgatgc
    36361 cttatttgaa atgttgggtg ctgataaatt taataagtat ttagaaagag gtactattga
    36421 ggttgcacca cttgcattta tgagaggtag aacacttgat aattcattta taatattgga
    36481 tgaagctcaa aatactactt ctgaacaaat gaaaatgttt ttaactagac ttggatttgg
    36541 ctctaaagct gtagttacag gagatgtaac tcaaacagat ttaccacaaa ataagaagag
    36601 tggtctaata caagctacag agatccttaa aggtgttcca ggtattgggt ctataatgct
    36661 aacagataga gatgttgtaa gacatgagtt ggtacagaga ataataagag cttatgagaa
    36721 gcacgacaaa agagaagaat ttaaaaaaga agaacgtaaa aaagtgaaaa tgcaggaaaa
    36781 gactttcaaa agaaagtaaa agtgtagtaa tatagatata tagcattatt aatgaataaa
    36841 aagtaggtgt agtgaatgga tttgatacta gatgatagac aagataaatt agaagtaagt
    36901 gaggagttaa ttgaaaagat aaaggatata atcatagaat gtttagatta tgaaggatat
    36961 gatgataatt atgaagtgag tctttctttt gtagataata aagagattca tgaattaaat
    37021 agagaatata gaggagttga tagagtgact gatgttttat catttcctct tttaagtgat
    37081 gattttgagg atgttgagtt agaagaagaa tccctaggtg acatagtagt atctcttgaa
    37141 agagccttag aacagagtat agagtataac catagttttg aaagagaagt atgtttttta
    37201 atatgtcata gtatgtttca tcttttaggt tatgaccacg atacagatga aaatacaaaa
    37261 gaaatgagag aaaaagaaga acacatatta aataagttaa acataacaag ggagtagttt
    37321 ttatgaaacc agaaaaaact cgtcaaggta taattaaagc atttaatgca gctatagaag
    37381 ggatattgta tacatttaaa tttgaaagaa atatgaaaat acattattta ggttcagttg
    37441 ctgttcttat aataagtttg ttttttaact tttcaaaatt agaaatgata atgttactta
    37501 tgtccatatg cttagttgta gttgccgaaa tgtttaatac agctatagaa aaagctgttg
    37561 atttagtaac tgatgaatat catgttcttg ctaagatagc aaaagatgtt gcagctggag
    37621 gcgttctagt ggcagcacta aattcagtag tagttggata tattttgttt tatgataaat
    37681 tgacagatat atctggaata ttaatttata aaataagaga atcagaatta catataacct
    37741 tgatatgtat attattggtt cttatagctg ttgtagttgt aaaagcattg acttctacag
    37801 gtactcctct aaaaggagga atgccaagtg gtcatgctgc cttagcattt gctatagcaa
    37861 ctgcaattac acttatgact gaaagagttg ttgcctctac tcttgcatat ataatggctg
    37921 tattagttgc ccaaagtaga attgaaggaa aaatacatac cttttgggag actattgctg
    37981 gagctttatt aggtgtgttg atagctatat tagtatttca gcttggaatg ttctataatt
    38041 aaaataaatg tacataaaat atttaaaata aaacaaatct gtgctaatta aatttttatg
    38101 atataatgta gagtattaaa taaaaataac ttttaattat tgtatacagg aggagttgtt
    38161 gtggataaca tagaattgtt aaggttggca gaagatgcca gacaacattc ttatgctcct
    38221 tactcaggtt ttagagtagg tgcagctctt ttaactaaga gtggtaaagt ttatactgga
    38281 tgtaatatag aatgtgcatc attaggtgga acaaattgtg cagaaagaac agctattttt
    38341 aaagcaatat cagagggtga taaggatatc tataaaatag ctatagcaag tgataactca
    38401 gaaaataatg agcaaacata tccatgtgga atatgcaggc aggtaattat agagtttggt
    38461 tctgatataa aaataataac gggatatact aaaggagagg tgtttgagca tagtataaag
    38521 gatttgttgc caaactattt ctctggaaat gatttaaaat aaatttaaaa gtataaataa
    38581 atttaagtaa ttaaaacaag aagataaaaa gttaggagac agttaatgtt caaatcaggt
    38641 tttgtcagta tagtgggtag accaaatgta ggaaaatcta ctttaatgaa taatgtagtt
    38701 ggagaaaaaa tagctataat gagtgataag cctcaaacta caagaaatac aatacaagca
    38761 gtatatacag atgaagaaat gcagatagta ttcttagata cacccggtat tcacaaacca
    38821 aaaaataaat taggtgaatt tatggtaaag gcagcaacag aagcttttaa aaatgtagat
    38881 ttaatattat ttgtagttga tgattctaaa aaaataggtc ctggagatag aaagataata
    38941 gaggatttaa gaagtgttaa gacaccaata attttagttg taaacaaaat agaccaacta
    39001 gaccaaaaag atgagctatt tgatataatc aaaatgtatg atagagaagg tatttttaaa
    39061 gaaatagtac caatatcagc attaaaagga aagaatacgg ataccttaat aaaagttata
    39121 caaaattatt tagaagaagg gccaaagtat tttccagatt atatgattac agaccagcca
    39181 gaaagagtat taatagcaga acttataaga gaaaaagttc ttcactatct taatgatgaa
    39241 atacctcatg gtgtagctgt tgaaattgaa aaaatgaaag cgagaaatga taaggaaata
    39301 gtagatgtat ctgctgtaat atactgtgaa agagattcac ataagggaat aataatagga
    39361 aaaaatggta ggaaactaaa aggaataggt aagtcagcaa gacaagatat agaattgctt
    39421 ttaggttcac aaataaatct tcaattatgg gttaaagtca aagaaaattg gagaaattta
    39481 caaaattata taaataattt tggatataat gataaataaa ggtgggttat atggatagga
    39541 aactggatgc ttcccaagat ttattatatg aagtaaaatc attaattgat aataataaag
    39601 tattggagtt aagagaatta atagaggaat atcatataat tgatatattc gatataatgg
    39661 aaaatttaga ggaagacatg aaaattcaat tatttgaagt acttccttta gatatggcat
    39721 cttctatatt agaagaaggt agtgtagagt tttttattag tattttatct aaactggatg
    39781 tagaacactc gaaaaatata cttgaactta tgtctcttgg agatatggca gacaagttga
    39841 gtgaacttga agaagaagaa agagaacaca taataaatct tttgaatcaa gagaatgccg
    39901 attatgtaaa agagttacta ttctatgatg aagattctgc tggtggaaca atgaccactg
    39961 gatatatttc tataaataaa gatatgacag ctcttgaggc tatagaccat atgagagaag
    40021 aagctgaaga agctgaaact atttattata tatatgtggt tgatgatgaa gaaaaactag
    40081 ttggagtatt gtcattaaga gaattaataa tagctagaga cgcaaatata gtagaagatt
    40141 tgatgagtga aaatataatc tctgtttatg tagatgaaga tagagaagaa gcagtaagat
    40201 tagtatctaa atacaattta atagcaattc cagtagtaga cagacaagaa aaattaaaag
    40261 gaataataac agtagatgat ataattgatg taatggaaga agaagcaact gaagatatgt
    40321 ataaatttgc aggaagttca gaacatgaaa gagaagttgc tgaaaaagaa aaccctacat
    40381 tgagagagca aataatatca gcacttagag gacgattacc ttggttaatt attaccttgg
    40441 ttggagggtt gttggcttcg ttaatacttt ctaacttaga ctatataatg aatcctgtat
    40501 atgctccatt agtatttttt atacctgtag taattggtat gggtggaaat attggaactc
    40561 aatcatcatc tgttacagta ataacattat ctaataagga tttaaatttt agcaatgtgg
    40621 ttagagaagg tattgtaggt attattacag gtcttttgtg tagtataata acaggtattg
    40681 taatttactt tgttatgaga aagttagaca ttgtattaat tgtttcaata tcattattta
    40741 taaatatggt tttaggtgca acaataggtg cgtttatgcc tgttttatta aaaaaaatgg
    40801 atgcagaccc atctacagtt tcatcaccaa ttatatctac agctcttgat ataactggta
    40861 tagcagttta ctttataata acaacggcat tattgtcaaa aattgtttaa taactttatg
    40921 tatgatttat cctcttctta ctaaaaatat aagtataaat gtaagaaggg ggttttttaa
    40981 aaatgaacaa tatcagttgg gaagttttta aaaaaacagg tagtatagat gcatatttat
    41041 atttctgtga ttgcaaaaac atacatgaag aaattgaaga agtaagggag aaaaaagaag
    41101 acgatgataa tcctgaacac ccaggggata gtacttaaag ctataaggta taaggaaagt
    41161 gacataatac ttacactatt tacaagaaag cttggaaaag tatctgcaat agcaaaagga
    41221 gctaaaaaaa ataagagctc tttgctttca tcatcacaat tattttctta tagcaatttt
    41281 accttaaaaa aacaaggtaa tatgtacaag gttactcaaa gtgaaataat aaaaagtttt
    41341 tataatatat cctatgatat agaggcattt tcttatgcta catatattac aaaattagtt
    41401 gaaaattcta tacttgaaaa tcagactaat aataggctat ttatattact tgcgcagact
    41461 ctgtatttgt atacacagga taatacagac aataggttta taacagcagc attcgagtta
    41521 aaatttttag actatatagg atttaaacct atagtaaata aatgcattaa ttgtgaatgt
    41581 aaaattttac aaaattctgt gtttaatatc tacgaaggtg gtatactatg tagtaagtgt
    41641 agtaaacttt ttgataataa tataaaatta gacttaacaa ctataagtct tatggaatat
    41701 gtattaagaa atgatatttt aacatgtagt aaagctaaag tatcaaaata tataacacat
    41761 gaacttgaga atattctaga caagtatttg aaagtttatg ttgataatat taattttaaa
    41821 tcattacacg tattgcaaag tgtaaaaaat aataagggag tggataatga tgagtagtaa
    41881 tataactatt gaaatggttg attcagtctt tgaaagagtt ccaggagcaa cttatgcaga
    41941 agcaaaagaa gcattagtaa tgtgcaatgg agatgtaatt gaggcagtaa tatatttaga
    42001 aaataaaaaa aatacttgta attgtaaagc aaaatctgct aaagaaacta tggaagaagt
    42061 atttggcaaa gatggagaaa atataaagtt tcaattaaaa gagcttctta gaaaaagtag
    42121 tgtagtgaga ataatagttg aaaaagatgg aaaaactatg atgaacattc ctttaacagt
    42181 tggagttgtt ggattagctt ttgtaccatt agctacttta gttggattat cagctgctgt
    42241 tataagtaaa tatcgtataa aagttcaaaa tgaagaagat ggagaaattg tagatttagg
    42301 tgaattgaat gaagaaaaac ttcacgtttt gaagaatatg ataactaatg cagcaaaaga
    42361 tgtgaaggat gtagtagttg acaaaaaaga agatgacaaa gatgtaactg ctgaccttat
    42421 gaaagaagaa tctgaaaaga ataaagaaga cgaagacgaa aataaataga tataaagtga
    42481 cttttaaaaa atgaagagaa gataattata agaattatca ctaacattga caaattttat
    42541 agaatttgat attctaaaag ttgtagaaga actattaaaa tagcctttcg tgtagacgag
    42601 aggctttatt gttataggag ggatttttat gaattttcaa gagatgatac tggcgttgca
    42661 aaaatactgg tcaaagcaag gttgtatcat gatgcaacct tatgatattg aaaaaggtgc
    42721 aggaactatg aatcctaata cttttttaag atcattagga cctgagcctt ggcaagtatg
    42781 ctatgtagag ccttcaagaa gaccagcaga tggaagatat ggtgaaaacc caaacagact
    42841 atatcaacat catcaattcc aagttatact aaaaccttct ccagataata tacaagaatt
    42901 atatttggaa agtttaaaag agattggaat tgacccaagt gagcatgaca taagatttgt
    42961 tgaagataac tgggaagctg ctacagttgg tgcttgggga cttggatggg aagtttggct
    43021 agatggtatg gaaataactc aatttacgta ttttcaacaa gtaggtaata ttgaatgtga
    43081 gcttgagaca ggagaaatta catatggatt agaaagatta gctatgtaca ttcaagaagt
    43141 tgatagtgtt tatgatttaa aatggaatga taaaataact tatggtgaag tatttaataa
    43201 agctgagtat gaaaactcaa tgtacgcatt tgaattatgt gatgcagata tgttgtttaa
    43261 tctgtttgat atatatgaaa aagaagcatt aagacttatg gaaaatggcc ttgtaatacc
    43321 ttcatatgac tatgtgttaa aatgctctca tgcatttaat acattggacg caagaggtgc
    43381 aataggagtt agtcaaagag cttcatttat aggtagagtt agaaatatgg caaagactgt
    43441 tgcagaaact tttgtaaaac aaagagaaga aatgggattc cctcttttaa aggacggtga
    43501 caaataatga aaaattatct tttatttgaa atcggtgttg aagaaatgcc atcaagattt
    43561 gtaggcagta cactagaaca attaaaaaac aatcttagta aattatttga tgaaaataga
    43621 atagcttttg ataaaataaa tgcgtatggg acaccaagaa gacttacttt agtagtcgaa
    43681 ggattgagtg atagacaaag tgatttagaa gaggaaataa agggtcctgc taagaaaatt
    43741 gctgttgatg cagaaaataa tttaacaaaa ccagcattag gatttataaa aagcaaagga
    43801 ttaagcgaat ctgacatata ttttaaacaa gttggaaaag aagaatatat atttgcgact
    43861 ataaagcaag atggaaaaga aacttcagaa gtactaaaag acatattacc agagactata
    43921 aaatctgtag tattcccaaa agctatgcgt tggggaggaa aaaatcttaa atttgcaaga
    43981 cctataagat ggatagttgc attgttaaat gataaagtat tagagtttga tttagaagga
    44041 ataatatctt ctaatgtaac aaaaggtcat agatttttag gtgaaagtac aatagaagtt
    44101 gattctatag aagattattt tgaaaaatta gaaaaaaatt atatagtttt agaccaaaac
    44161 aagagaaaag aaatgataag aaaacaatgt atagaagtag caaattcttt aggtggagaa
    44221 gttgaatttg atgaagattt acttgaagag gtaacttatt tagttgaata tccaactgct
    44281 ttttatggag aatttgatgt ggattatata aaactaccaa aagaagttgt tataactcct
    44341 atgaaacaac atcaaagata ttttccagta cttaaagaag gaaaactgtt acctaacttt
    44401 atagctgtta gaaatggtga ttcttacaga atagacaatg ttaaagctgg aaatgaaaaa
    44461 gttttagaag caagacttgc agatgcttta tttttctata gagaagatac taagagaaac
    44521 ttagaaagct acatagaaaa attaaaaact gttgtattcc aagtaaaact aggaactatt
    44581 tacgacaaaa ctttaagact tgaaaaatta agtgctgata tacttgatag tttaggttta
    44641 gtagatgaaa aggcagatac tataagggct gctaaattat ctaaggcaga tttagttact
    44701 ggaatggtag gagaatttga tgaattacaa ggttttatgg gaaaagaata cgcaaaagtt
    44761 ggaggagaga atgatgttgt aagtgaagct atttttgaac attatcttcc aagatttgca
    44821 ggagatatac ttcctaagac taatgcaggg gttgccttat caatagctga taagttggat
    44881 tcaatagcag gtttctttgc tataggaata caaccaacag gttctcaaga cccatatgct
    44941 ttaagaagac aagctttagg tatacttagt atacttatgg acagaaaagt tgctgtggat
    45001 ataaaagtac ttattgaaca tgctttagat aattattctg aattagattt taataaagaa
    45061 gaagttgttg agcaaataat gaatttcttc aatgagagag taaaaaatct atttaaagat
    45121 ttaggaataa gatatgatgt tgttgatgct gttttaagtt ctgatataaa tgatgtttct
    45181 gatatgcata tgagagcttt agagcttaat aattggctag aaaaagatga attagtggag
    45241 atgcttactg catttaatag agtttctact ctagctcaaa aagcagaaag tgataaaatt
    45301 gatgaatctt tattaaaaga agatgctgaa attagacttt acaatgaatt taaacaagta
    45361 aaggtaaaag ttaatgaatt attaaaggat aagaaatatg gtattgcttt agatgaattt
    45421 gcatctttaa gaccagtagt tgataattta tttgatacag ttatggttat ggataatgat
    45481 gaagctatta agaacaatag acttgcttta cttaaacaag tatatgatac tatgctagaa
    45541 atttgtgatt tatctaaaat tgtttataaa taaaatttaa actatttata agaaataagg
    45601 ctgtctcaaa atagagatag tcttattttt cattgttgag atgatgaaaa tatatttgat
    45661 ttcatagaaa aagagtgtct cataaactaa aaagttcatt ttgagacacc catgcttttt
    45721 gtaagtaaat ataataaatt atattttata cttatcagtt attttgttta aaatattatc
    45781 atttgaattt acccattcat aaaaattgcc tgatgatgcg tccgatattt tataaagcat
    45841 ttcaggaata tcactagctt taaaatctcc aagtgatttt cccaatctag tcttacctat
    45901 ttcaaaactg cctccaacaa ataattcaaa tgcatctgta gatataccat caacattctt
    45961 ctttttacca cataaaccta tgctaccaac ttgatgaaca ccacatgaat ttaaacatcc
    46021 tgatatatat aacttaggtg ctttatttaa aatttcttca ttattttgta atctaaaata
    46081 atctatgatt tcatgtaaca ttttctgact attttgtatg cccatttgac aaattggaac
    46141 acctatgcaa gatatacttt gttctaattg agaattacaa ctaatagttt tacttatttc
    46201 taatattctt tttgcttcat ttccatttaa atttaaaata tataatccct ctgtcattcc
    46261 tagtctaatc ataggatttt caacattatc taattcttta agtaaagtac ttaaatcctt
    46321 tgttaacaaa atacctccaa ctggatgtat atatacagtg tataaaccat cctgtttttg
    46381 aggtattagt ctagaatctt gtatatctat tttaactcca ggtttagagt aatctatttc
    46441 ttcaatattg agttcaagac taccattttc tttttctaat ttatagtatt ctttaaattt
    46501 ttctaagaat agttcttcac caagtgattc gaccatatat ctaaccctag ctctgttttt
    46561 attttcataa tttccataat ccataaagaa tttaatcata ccttcaacac aatataaggc
    46621 gtctttcggc tttataagtt catcaagttc taaagcaact ttgggttgtt tacctagacc
    46681 tccacctaag aaaactctga aatatggttc atcatttttc aaagtagcaa caaatcctaa
    46741 gtcttgtata ctacaatgag cagtatctgt gtaacaatta gaataagaaa cttttaattt
    46801 tctaggtaaa tggtatgtag tagcattttt gataaaatat ttatcagtag caactgcata
    46861 tggagtaaca tcaaagactt cactagggtc aactccagat aatggagata atccgacatt
    46921 tctaggataa ttaccaccac cacctctagt gaatatatca ttttttattc cttcttccat
    46981 gatatcaaca attgaattta tagataaatc gtgtaattgt atagcttgtc tagttgttaa
    47041 gtgtatctta tctaagttgt gtttaatagc tatttcatag attttattaa gttgatactg
    47101 agataaaact ccagcagata ctctaagtct tatcataaaa gattttttgt ctctttgagc
    47161 ataaacacca tatccaccag aaaatccctt atattgcatt ttatttattt ctccatttaa
    47221 aaattttgaa gaatattctt taaaattagg tagttcattt aacaatatct ttttaaaatt
    47281 atccattatt tcacctcatt gttagtcttg tataaaaata tatctattgt ataatagata
    47341 tatatattat tatctagtca ggataaaaat ataacagaat aattctatta aaagtaagaa
    47401 taataaggac atagtgagta ttttgttttt attttacaaa ttaagtataa tatttttgtt
    47461 ttataaaata taattaagat aaatagataa ttttgtaatt ttaatatata atatttagta
    47521 gagtgtggga gtagcactta atatttgcag gaggagtttt tgtatatggg agaaataata
    47581 aatgcattag atagagccct agacataatt ttgctgttat atcatgaaaa gagagaaatg
    47641 ggaataactg aaataagtaa ggctatggga gtatataaaa gtactgtaca tagaactttg
    47701 gttactcttg aaaacaaagg ttttgttata caaaatgcag agaatggtaa gtattggcta
    47761 ggaataaatt tatatgcaat aggtatggtt gtgggagaaa aaatgtcatt gactgaaata
    47821 gtaaaaccat atacgaaaaa attaaatcaa gaatttaacg aagtagttaa tgtatctata
    47881 ttagaagaaa gagctcaaga ttctccaaga agtatcataa tacataaaga gtatggttca
    47941 aatcaattac tttcagtaaa tccatccgtt ggttcaagta gtgaatgtta ttgttcagca
    48001 gttggtaaat gtcttatggc atttaatgat tctatagatt ttgaaaaata tagaaaaaca
    48061 ccaatacaca agtatacaga acatacaatt gataattggg atgatatgat gcttttttta
    48121 gcaaaaataa aagaacaagg atatgctata gatgatgaag aactagaaca tggactaact
    48181 tgtattggag cacctatctt agataaaaat aataaagcta ttgcagccat aagtttgtca
    48241 ggtccaacta taagaatgag agaaggggac tttgagtata agattaaaag agttatagaa
    48301 actgcaaaaa gtatatctga actttttaga tgatttttaa tctcgataat aaaaataggc
    48361 tatatttaaa ttgttatttg taaatatcag tttaaatgta gcctattttt ctttaatcta
    48421 aaattaaata ttcttaaaat ttaaaatgtt attgaaatat ataagcgaat gtgttatctt
    48481 ttaattaagc aataaaaata gaacaaggtt ctatactata gaacaaaatt gatgcatata
    48541 tagtatttat acaaaatata aatattatat atttaaaatt aagtaaaaat attacataaa
    48601 ggggagattt gagaaatgga aaaagtcaag ttgagtgttg aaagatgtaa gggatgctat
    48661 ctttgtattg aagcttgcaa aaaagatgca ataagtcttt ctgactacac aaataaaaaa
    48721 ggctttgtaa ctattaaagt cgatgaagaa aaatgtattc aatgtggcgc ttgttataca
    48781 atgtgccctg atttagtatt tgagatatta taaattattt ttattagagg ggagaaaagg
    48841 tatgcaaagt ttattaatta aaggaaatga agctttagca gaaggtgcta tacgtggtgg
    48901 ctgcagattt tatgcaggtt atccaataac accacaatca gaaatattag aaatgatggc
    48961 atggagacaa aaagaagttg gaggagtatt tatccaaggt gaatcagaaa tagccagtgt
    49021 aaatatggtt atgggagcaa gtgcacttgg agcaagagct atgacttctt catctggtcc
    49081 aggattttca ttaaaacaag aaggtattgc atattgggta tcagctggtt tgccagcagt
    49141 tgtagcatgt gtgcaaagat atggtatagg tgatggattt ataggagctg gtcaagataa
    49201 ttattggcaa gcagtaaaag gcggtggaaa tggagattat catttaatcg tgttagcgcc
    49261 taacagtgtt caagaaaata tggatatggc atatgaatct tttgaattgg ctgaaaaata
    49321 tatgcatcca gtacttatac tttctgatgc tacaataggt caaatggtag aaccttgtat
    49381 agcaccacca atgaaagaat acgatatgga taaagctcca tgggctgcaa aaggaacacc
    49441 tgtaggagaa aaaaataaag taataactga tataacatat tttgaagacc aagatacatg
    49501 gcaaaaaggg tatatagaaa aaatgagaaa agttcaagaa gatgaacaaa gatgggaaga
    49561 gatagaaata gaagatgctg aagtggtatt tgtggcatac ggtatttcat caagaatagc
    49621 agaaggtgca gttagagatg ctagagcaaa aggaatgaaa ttaggtctaa taagaccaaa
    49681 aacattgtgg ccttacccaa gaaaagcatt taagaaatta agtgattcat tgaagggact
    49741 tgtaacagtt gaaatgaaca tgatggcaca gatgaaggaa gatgtaatta tagcatgtga
    49801 taatagattt ccagtatatt cagtagcaac agctcaatat atggcagaag taaatcaatt
    49861 aatagatttt gctcaacaga ttatagatgg aaaagctaag caagaggagg tattttaatt
    49921 atggctaaga aagcaccagc aataatgatg tgtgaaaata tgttttgtgg aggctgtgga
    49981 cactctatag tcgatagaat tattggagaa gtattaacag aaatggatat atgtcaagat
    50041 acaattatat gttcagatat aggttgtaat catatgtttc aatttagtat gaatgtagat
    50101 gttgtcgttc cacctcatgg taaaatgggt gcagcaatgg cagctatgaa aagagttaga
    50161 ccagataaat atatatttac aatagctgga gatggtggag cttatgcgat agggcttggg
    50221 gaaactatgt cagctgctac tagaaatgat aatgttatat tccttgttat gaacaataat
    50281 atgtttgcaa tgacaggagg gcaaatggct ccaactacta tagaaggtca atatacagct
    50341 tctactccag cgggtagaaa ttacgcagat catggttcaa catttgatgt tgtaaaagtt
    50401 atgggaaatt tagatatagc atatctagca agaggaacaa taactaaccc taaagaaata
    50461 aatacaacaa aaagatacat caaaaaagct ctagaaaaac aaaaagaaaa gaaaggtttt
    50521 tgcttaatag aagtgcttgt accatgtcca acaaactggg gattaaagcc agtagatgct
    50581 atgaagagaa ttgaaaatga acttgttcca aagtatccac ttggagaatt tattgattag
    50641 ggaggaaata atatgttgaa tgaaattata tgtgctggtt ttggaggaca gggagttctt
    50701 acagcaggaa aaattataat gtatgttgca tataaggcag aaaagaaaat tacatggttc
    50761 ccatcttatg gaaatgagat gaggggagga agtgcaaatt gcaatgttgt tgtaagtgat
    50821 gaaaaaattg caaatccttg tgtaaaaaat ccagatatac tattgggaat gaacagcgta
    50881 tcagtagata agtatgaaag tttaatgaaa aaaaatggtt atatgtttgt caatgaatca
    50941 ctagtaagta aggatagaaa gtacagagaa gacattaatg ttataaaagt acctgttact
    51001 gaaatagcac aaaatttagg caatgaaaaa gctgctaaca tttgcatgtt aggtgctatg
    51061 gtaaagagtt tggatttatt tacaaaagaa gaatttgaaa gtggtatgtg tgaatacttt
    51121 gaaaatcaag gcaaaggtaa attcaattct aaaaatgtag aagcatttga agcaggatac
    51181 gactacgaat gaatatttta aatatttaaa ataattattg aaatacttat ttttatatgt
    51241 tagtatttaa ttaataaaat atagaacgtc gttctggaca ataggacgaa gtctttaggc
    51301 cggaatacaa gaacgacgtt ctgtatagta gaactagggg gtgttaaatt gaaagtttta
    51361 gtaataaatc ctggtggtac atccacaaaa atttctgttt tccaagatga aaatgaaatt
    51421 ttgaaaaaaa atataaccca tacaagagaa gatttaaaaa atttttctaa agtatttgat
    51481 gaatatgatt atagaaaaca actgatagta gatattttaa gtagtgagaa tcatagtata
    51541 aattcttttg acgcagtagt tggtcgtggt ggacttatga aggctataaa aggtggtacc
    51601 tatactgtga gtgaagaaat gattgaggat atgagaaatg aaataaatgg ggaacatgca
    51661 tcaaatttag gtgcattatt agctaaaact atagctgatg aaataggagt acaatcattt
    51721 gtagtagacc cagtttctgt agatgaattt gatgatgtat cgaggattac aggaatatca
    51781 gacatagaaa aagcaagttg gttacatgca ttaaaccata aagctgtttc aagaaaaatt
    51841 gcacaaaaac tagggggaaa atacgaggat tataacttta ttgtagctca tttaggttca
    51901 ggaatatcga ttgtagcaca taaacaaggc aaaatgattg atggaagtgg tggtaggtct
    51961 gatggtccat tttctccaga aaggagtggt ggattacttg catatcctct tatagaattg
    52021 tgttactccg gaaaatatac tcatgatgaa atggtcgcaa aagtaagttc gattggggga
    52081 atgtacgatt atttaggaac aaaggacatg atagaaattg aaaacagaat caaggagggt
    52141 gatgaaaagg caaaacttat aatggatgca tttatttatc aagtagcaaa agaaatttct
    52201 atgtatggag cttcactttc agggaatgta gatagagtta tcttgacagg agggatatct
    52261 cactcagaaa tggtagtcaa aggagtagca gaaaaagttt catatctagc tccaatagag
    52321 atagtaccag gagagatgga aatggaggca cttgctctag gagcacttag agtacttaat
    52381 ggagaagaaa tagcaaaaac ttattaatat ttaggagaaa gagtatgtta aaagatttta
    52441 ataatttatt agaagaggct aaaaagcttg aacctgttat tgtaagtgtt gctgttgcag
    52501 aagacaaaga agtattaaag gctgtgaaat cagcaacaga tttaaacatt ataaagccaa
    52561 ttcttgtagg agacaaggat aaaatagaaa gtatatcaag agaaattgga ttaaaaggtc
    52621 atgaaataat aggttgtcaa aatcccgatg aatcagttaa aaaagctgta gagatagtta
    52681 aaaataagga agctaaagtt cttatgaagg gattagtaaa tacaagtgtc tatatgagag
    52741 ctatattaaa taaagaaacg ggtcttagaa caggaagatt attaagtttg cttgcagtat
    52801 atgaattacc tcaatatcat aaacttcttt attgtactga tagtggaata aatgtatcgc
    52861 caaacttaaa tcaaaaaaaa gatataatga caaatgcact tcttactatg aaaagtatag
    52921 gcatggaaaa tcctaaaact gcgatactta ctgcaaatga aatggtagac cctaagatac
    52981 aatctacagt ggatgccaac ctacttgtag agatggaaaa atcgggagaa ataccaaagt
    53041 gtatagtgga aggtccaata gcatttgatg tagcatttga tgcacatgca gcagaacata
    53101 aaggtattaa aagtaatatt gctggtgatg ttgatttatt agtatttcca aatatagaag
    53161 cagggaatat attaggaaag tcatggctta gatttaataa agctaagtgg gcaggtatag
    53221 ttttaggagc aacaaatccg ataattttag gttcacgttc tgacacagct gaaataaaaa
    53281 taaattcaat agcattagcg tgcctagcat cacaataaga aattattatt aaggaggaga
    53341 aagtattatg aaatatgcaa aaagaatgga tatggtaaaa gcttcagcta taagagcttc
    53401 tcaaaagaaa atagctcaga aggtggcaag tggtggaact gttatatctt ttgctgcagg
    53461 gcttcctgac ccaaatttat ttccaataga agagataagt gaaataactc aaaaagtact
    53521 taatgaaagg ggaaaatttg ccctagcata tggacctact aaaggtgaag atgagttgtt
    53581 agatctatta gtaaaaagaa tgaaagaaga agaaaatatt gatacaaaag cagaaaatat
    53641 tataataact actgggtcac aacaagggat agcattaagt gctatgattt tactagaaaa
    53701 aggagatata gtagttacag aaaatccaag ttacttaggt gcaataaatg catttagacc
    53761 ttatgaatgt gattttttag gagtagatac tgatgatgaa ggaatggtaa cagatgattt
    53821 agataaatta ttatctgaaa attctaacat aaaggtaatc tatgtaatac caacatttca
    53881 aaatcctaca ggaaaaacat ggtctttaca aagaagaaaa gattttatgg aagtcgtgaa
    53941 taagtatgat gtcatagtaa tagaagataa tccttatgga gaaataagat ttacaccaga
    54001 gcctctacct acacttaaat ctcttgatac taaagataaa gtattatact taggttcatt
    54061 ttcaaaagta ttatgtccag ggtttagagt tgcttggatg tgtggtaatg tagacatcat
    54121 agataaggca gaacttttaa aacaaggtat agatttacag tcaaatcaat tttcgcaagt
    54181 acaagtaata gaatttttaa agaaatacaa cttaaatgaa catatagaaa aaataagagc
    54241 agagtataag aaaagatgtc tgctaatgtt agatgctatg aagaaacatt tcccagagga
    54301 agctaaatat acagaaccag atggaggaat gtttatatgg gttgaacttc ctgaaagtat
    54361 aaatgtagat gatttattgg ataaagccat tgatgctgga gttgcttatg tttctggaga
    54421 gtccttcttt gcaaataatg gtccaaaaaa tactatgaga cttaactata caacaatgtc
    54481 agaagaacaa atcgttaaag gtgttgagat acttgcagaa gttataaaaa aagaactcaa
    54541 ttatgcttgt gaataaatat aaaaagtgag ggtttaaata tgggagaaaa agaaaacggt
    54601 ataaagtttg gaaagttaat gtttcaactt gttattatag caatatcttg ggaattagtt
    54661 tatattatac catttatcca atatacatta tatgatccaa tcctaaaagc attagaatgt
    54721 acaaacactc aactaggttt tctacttaca atttatgggt taggtaatat atttggagct
    54781 cctataggag gctggttagc agatagattt gattatagaa agataatttt aggttctgta
    54841 tttcttaatg gagtagtatc atttttattt gcatttaata tgaactatcc atttgcagtt
    54901 gctacttggg taggttgtgc aatcacatca ttagttatga actatccttc tatggtaaaa
    54961 atacttagag ttataggaaa agataatcaa ggtaaagttt atggattcaa tgaagctatg
    55021 gttggagttt ctggcgtagt aatgggagca atattcttat atatttatac atgcttttca
    55081 actcctgtat tgggtatgag atgggtaatg atatctttag gaatattatc aatagtgatg
    55141 tgtccagtat tatggtttgt aattaaagat gtagatatca gagaagacaa agaagaagaa
    55201 gcaagtgaaa aaatgtctgc tggcgatttt atgacagtac taaaatcacc aaacacttgg
    55261 ctagtaggaa taagcatatt ctgtgtatat tcattcacag ttacaatgtc ttattttaca
    55321 ccatatataa catcagtact tggaggttct gtggcattgt ctggggcatt agcaataata
    55381 agacaacatg gtctaaaatt gtttggagca ccatttggag gatattgtgc agataaagta
    55441 aaatcaccta caaaagtact actaccaata tatgtatttg gtatagcagt aataatttta
    55501 ttcctagtac tacctgcatc tactccaatg actatcttta tagctttaac atttgtagta
    55561 ggtatattag gttacatggg aaaaggtata tactatgcag ttcaagatga agtaaaagtt
    55621 ccagttaaat attcagcaac tactataggt atagctgcag ctttaggatt ttcaccagat
    55681 gtattccaat ttgcattaat aggacactgg atagatactt atggcaataa aggttacaca
    55741 tatacattta tattccagat agcaattcta ataattggta tactatcttg tttatatata
    55801 ttaaaagtta agaagaaaag agaaagtaag ttagaagcat aaatattgta aaaataccaa
    55861 attattaagt tttatagcaa tacttaaaat ttaaagtgca acacaccaaa atgttatcaa
    55921 gttttctagg ttttagtata aagttacccc taaaggtctg gaattgagta gcaattatca
    55981 tctatttata tatttacata aacctattta atttaaatta aaagaatttc aaaaataggt
    56041 ggtaattgta ctcaatttta cacctcataa taaatacgaa aatttaaaat ctaactatat
    56101 actattcaat tatatattaa taattgtata tggtactatt tatatagtaa cagtttcagg
    56161 cttttcatta catgtattcc aatttatatt gtttgattat ttgtcaaata catatcgtta
    56221 aaaagtttat aaatttacgt ttatatatcg attaatattc tttgttttag agatagatac
    56281 ttatagcttg tggattaaaa ataatataaa tagaagatag attttttaga tatattgagt
    56341 tgaagattat atagaatgtt gaaacatagc tatttaaatt attgtctata aatataaaat
    56401 gaatggtggt gagttaagtg accgatttag ttaacaccat taatggaatt gtttggtcac
    56461 ctgtattagt tgcaatgtgt ttgtgtatag gattatattt ttcaatgaaa ttaagatttt
    56521 ttcaaataaa agatgtaaaa gaaatgttta ctcttttgct tgaaggagat agctctcatg
    56581 ttggaatatc ttcgttccaa ggttttgcta catctatggc aggaaggata ggaactggaa
    56641 atatagctgg tgtagctgta gcgatagcaa tgggagggcc aggagcatta gtgtggatgt
    56701 gcataatggc tacattaggg tcggcaactg catttgtaga atctacttta gctcagatat
    56761 ataaagatga gcatgatgga cagtacaggg gaggtcctcc atattacttt gaaaaaggtc
    56821 ttggttggaa aaaatgttca attttatttt cagtggttgc aatcataagt tatacatttt
    56881 ttatgcctgg cacacaatca aatactctat atctagcaat gaatcaagca tttggatttt
    56941 caaaaatgac aatagcagtt acatcttcta ttgcactagg tataataatt tttggaggaa
    57001 ttaaaagaat tggtttattt gctgagaaag tagtacctat aatgggtgga gcatacctta
    57061 ttatgacatt ggtaataatc atagcaaata taaatctagt tccaggagct attatgacaa
    57121 tgtttaaatc tgcatttgga tttggttcgg tttttggagg gatgttaggt tcagcaatat
    57181 cttggggagt aaaaagaggt atatactcaa atgaagcggg agaaggttct ggaacttatg
    57241 gtgcagcagc agcagaagtg agtcacccag ccaaacaagg attagtccaa gctttttctg
    57301 tttatgtaga cacactatta atatgtatgg ctacaggtat tatgattata ataacaggaa
    57361 tgtataatgt tacagatgtt tacacaacat taccaggtgt agaaccaggt ccacaatatg
    57421 tacagagtgc aataaataca ttacatcctc aattaggtga tatttttata gcaatagttt
    57481 tactactatt tacatttaca acattattag ccttctcata catgatggag accaacgtat
    57541 cttacttagt gactagaatt aaggcagaaa aacatgaaaa gcacataatt aatgtctgta
    57601 gatttatgct tataatatgt gcagcaatgt gttgctttat agaaccactt acaagttggg
    57661 cacttggtga tatatgtata ggtgtattaa catatataaa cttagttggt atatttacac
    57721 taaaaaaacc agctataaaa gcatattacg attatcaaag gcagaaaaag atgggagtaa
    57781 aaaggagcga tagaactttt aatcctatag aattagatat aagaaatgct gagttttggg
    57841 aaaatagata cttaaaagag gataagcatg agttaaatta aagttataat aaaatataaa
    57901 aattactaaa taaatttagt gggaggtaag attggtatga aagaaagaat aatacaagtg
    57961 gctgattcaa agaaagaaaa aattctaggg ctatgtcaat ctttatatga tgaaccagaa
    58021 attgctttgc aagaatataa atctgctaaa aaaatatcag aatttctaag agaagagggt
    58081 tttgatgttg aggaaaactt ggcaggtatg gctacagctt ttaaagcaac caagaaaaat
    58141 ggagatggtc ctaaaatagc atttatagca gaatatgatg ctcttccagg caatggacat
    58201 gcatgtggac atcatttaat tgcatctatg ggagttggag ctggaatagc tctttcaagt
    58261 atactggata cttacaaggg agaagtaagt ataattggaa cacctgctga agaaactgga
    58321 gatggaaagc cttacttgat agaacatggt gtatttgatg gctatgatgc agcaatgatg
    58381 atacatccaa attcaaaaac ttgtgtaaca cctgaaatta tagcaatagg aggtctagat
    58441 ttcatattta ctggaaaagc atctcatgca ggagctaagc catataacgg aataaatgct
    58501 ttagatgcag tagtactact atataataat ataaatgcac taagacaaca attagtagat
    58561 ggaacgagaa tacatggtat tatactagaa gcaggaactg ctgcaaatgt aatacctgat
    58621 atgggaaaag taagattaga gataagggca aaagaacaaa attactttga tgaagtagta
    58681 gaaaaagtga aaaattgtgc tagaggtgct gcaatagcca ctggatgtga acttgaattt
    58741 tatcactttg aaccaacttg ccaaggatta aatgaaaata aagttttggt agacatattt
    58801 acaaagatta tggaggaatt tggaatatat gaagatgaac aagtaatgtt aggttcaaca
    58861 gatatgggaa atttaagtca aataatgcca tgtatacatc ctttgatgaa gttttctgaa
    58921 aatggagaag aactacatac aaaagaattt ttggaggctt ctataaattc atatgcaaaa
    58981 gatagagtaa ttgatggtat aaaaatatta gctttaacag gatttaattt atttgaaaat
    59041 ccagagttat tgaaaaaaat gaaggaagag tagaacttag actattttta attatactct
    59101 ttaaaataag aagagtgtct caaaatgaac tttttagttt atgagacact cttttttgca
    59161 tgaaattaaa tatattttta ttattttaac aatgaaaaag aggcttatct gtttttatat
    59221 aacctttttt aacttgtagc acaaaatatc atgaatgatt acaattcgaa tgataaaatc
    59281 gattataaga tatataggag gggtacatat ggaaacttta gaagtaagaa atattgaatt
    59341 gagtggtaca aattatgaga taggatacag actaggagaa ttggttgcta atatgccaga
    59401 gataatagaa ggtcagatta ataaatctaa tctggtaagc aaaaaagaag aaaaagagat
    59461 gatagagttg tttgataaat attgtccagg tcttaatgaa gaattgcaag gttttgctga
    59521 tgcaattcaa gtgaattgta atcaaatttt atattatact atgacttatc ttaaaccagg
    59581 ttgtagtcaa gttgcattaa caccagaatt aactgaaaat gggcatgtac tatttgcacg
    59641 taatttcgat ttctcacaca atatggagga ttttgtttta tgtaaaacta aagtaaatgg
    59701 aaaatatgca catataggta ctaccgttat gcaatttgga agaggtgaag gtatgaatga
    59761 atgtggatta ggtgtaagtc agtcatcatg tggtattcct gtaggcaatt cagatggttt
    59821 gagaaaacca gcaattgtag gtttacagtt ttgggctgtt attagatatt tgttagaaaa
    59881 ttgcaaagat gtagatgaag cattagaata tttaaaagat atgccaattg catacaatat
    59941 taatttattg cttgcagata agtctggaaa cattgcactt gtggagactt tagatgggaa
    60001 aaaagaagta aatataatca acagtttaga aagtaagaga gaatattttt tacactctac
    60061 taatcataca catattgata aacttcataa attagaacca caatcaatga aaaattctat
    60121 tcatagatat aaattaatta aagaatatat aaataaggct aaaaaaattg gagagaaaga
    60181 gttaatgaat ttattgtctt taaagtatcc aaatggctta tcttgtaatt actataatga
    60241 tttttttgga actttaaaaa gtatagttat ggatttaaat ataggaaagt ttaatatttt
    60301 atggggtgga ctagagaata aatgggagag ctactattta aaaaatgaca ttaaatctat
    60361 aacacagagg ataaacataa atattgaaaa agcaccatct gacttttttg atttcattga
    60421 ataaaaaatg tatttaatta ttctaaataa ttaggctata taaatcaact aaaagttttt
    60481 atttatcgta ttgaattagt tataatattt tagtatagta aaaaatagtg ctattttaca
    60541 tatttaaaga gtttaagtag aagagaggtg ccatattgga ttacttaaag tattttgaaa
    60601 aagctgtaat gtatattgag aataatttac atgaaaatat aactgtaaat gatgtggcaa
    60661 aagaaactgg atattcttat tatcatttaa cacgcttatt taagtctatg tttggtgaga
    60721 gtgtaggaag ttatataaaa aaaagacgat tagtaagtag tacaaaagaa cttttatata
    60781 gtgataaaaa agtgatagat atagctattg gtagtggatt tgaatcttcg gaagcattta
    60841 gcagagcatt taaatctata tacaaggtta gccctataga atatagaaaa aatagaattg
    60901 atgtatttgt tggtaaaaag aaaaaattag agttagattt tatgagacat cttgttggaa
    60961 atataacgat taaaccagtt ataaaagaaa tagaaaaaat aaaggtaata ggtataaagg
    61021 ataaagtaat tttagaaaat aattcattac ctgacttgtg ggagaaattc agaaaagtac
    61081 atcatattgt cccaaatact ttgccatcaa agagagtttt tggaatctgt gaagcaatct
    61141 ctgaaataca cctagttagt gaaagtatgg aatttaatga gataatagga cttgaagtta
    61201 atagttatga tgtgattcca aattcatttg tttcaaaaac tattaaggaa ggaaagtatg
    61261 ctatatttac tcatactggt agcttagata acttagacaa aacttatgaa tatatatggg
    61321 gtacttggtt tttaagttca aaagaagaac tggatataag agatgatttt gaagtatatg
    61381 atgaacgatt tttaggtcct agtaatgtga actcacaaat tgatatttat atacctataa
    61441 aataatctat aagaaaacta aaagataatt tgattaagaa atagatgttc gtaaaagagc
    61501 atctattttt tatttttatc agatttagat aattttaata ataaaagaaa taaaaataag
    61561 ttcttaaatt
//
LOCUS       DAMKPG010000010        59237 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMKPG010000010 DAMKPG010000000
VERSION     DAMKPG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 59237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.012"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(80..2143)
                     /locus_tag="PC621_000712"
     CDS             complement(80..2143)
                     /locus_tag="PC621_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF4107898.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2524..5859)
                     /locus_tag="PC621_000713"
     CDS             complement(2524..5859)
                     /locus_tag="PC621_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF4107899.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5909..6337)
                     /locus_tag="PC621_000714"
     CDS             complement(5909..6337)
                     /locus_tag="PC621_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107900.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6327..6707)
                     /locus_tag="PC621_000715"
     CDS             complement(6327..6707)
                     /locus_tag="PC621_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF4107901.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6740..7048)
                     /locus_tag="PC621_000716"
     CDS             complement(6740..7048)
                     /locus_tag="PC621_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107902.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(7081..8265)
                     /locus_tag="PC621_000717"
     CDS             complement(7081..8265)
                     /locus_tag="PC621_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF4107903.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(8269..9732)
                     /locus_tag="PC621_000718"
     CDS             complement(8269..9732)
                     /locus_tag="PC621_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF4107904.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(10242..11111)
                     /locus_tag="PC621_000719"
     CDS             complement(10242..11111)
                     /locus_tag="PC621_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF4107905.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(11131..13047)
                     /locus_tag="PC621_000720"
     CDS             complement(11131..13047)
                     /locus_tag="PC621_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF4107906.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(13050..13970)
                     /locus_tag="PC621_000721"
     CDS             complement(13050..13970)
                     /locus_tag="PC621_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF4107907.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(14072..14824)
                     /locus_tag="PC621_000722"
     CDS             complement(14072..14824)
                     /locus_tag="PC621_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4107908.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(15121..15738)
                     /locus_tag="PC621_000723"
     CDS             complement(15121..15738)
                     /locus_tag="PC621_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107909.1"
                     /translation="MNVIKKFIFVILIINILGCGISLILQSSLGSDAITLLNEGIHIK
                     LGISYTIAGLIYNGTLLLIALLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSL
                     GIIIRIMSFIIGHILMCSGFAMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLI
                     FLILGVYLGGNLGFGTIFSILCTGTTISIVSKMISVNKKRYYSSI"
     gene            complement(15826..17283)
                     /locus_tag="PC621_000724"
     CDS             complement(15826..17283)
                     /locus_tag="PC621_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF4107910.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
     gene            complement(17305..17658)
                     /locus_tag="PC621_000725"
     CDS             complement(17305..17658)
                     /locus_tag="PC621_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4107911.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(17687..18934)
                     /locus_tag="PC621_000726"
     CDS             complement(17687..18934)
                     /locus_tag="PC621_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4107912.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            complement(18942..19259)
                     /locus_tag="PC621_000727"
     CDS             complement(18942..19259)
                     /locus_tag="PC621_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4107913.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            19507..21420
                     /locus_tag="PC621_000728"
     CDS             19507..21420
                     /locus_tag="PC621_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4107914.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(21543..22697)
                     /locus_tag="PC621_000729"
     CDS             complement(21543..22697)
                     /locus_tag="PC621_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF4107915.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(22979..25594)
                     /gene="mngB"
                     /locus_tag="PC621_000730"
     CDS             complement(22979..25594)
                     /gene="mngB"
                     /locus_tag="PC621_000730"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF4107916.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(25738..27663)
                     /gene="mngA"
                     /locus_tag="PC621_000731"
     CDS             complement(25738..27663)
                     /gene="mngA"
                     /locus_tag="PC621_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF4107917.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(27793..28518)
                     /locus_tag="PC621_000732"
     CDS             complement(27793..28518)
                     /locus_tag="PC621_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4107918.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(28781..29560)
                     /locus_tag="PC621_000733"
     CDS             complement(28781..29560)
                     /locus_tag="PC621_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF4107919.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(29553..30980)
                     /locus_tag="PC621_000734"
     CDS             complement(29553..30980)
                     /locus_tag="PC621_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4107920.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            31371..32093
                     /locus_tag="PC621_000735"
     CDS             31371..32093
                     /locus_tag="PC621_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4107921.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            32290..33957
                     /locus_tag="PC621_000736"
     CDS             32290..33957
                     /locus_tag="PC621_000736"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF4107922.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(34053..35372)
                     /locus_tag="PC621_000737"
     CDS             complement(34053..35372)
                     /locus_tag="PC621_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4107923.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(35413..36159)
                     /locus_tag="PC621_000738"
     CDS             complement(35413..36159)
                     /locus_tag="PC621_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF4107924.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(36403..38412)
                     /locus_tag="PC621_000739"
     CDS             complement(36403..38412)
                     /locus_tag="PC621_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107925.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQSFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQGVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(38850..40283)
                     /locus_tag="PC621_000740"
     CDS             complement(38850..40283)
                     /locus_tag="PC621_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF4107926.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(40370..42286)
                     /locus_tag="PC621_000741"
     CDS             complement(40370..42286)
                     /locus_tag="PC621_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4107927.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(42360..43220)
                     /locus_tag="PC621_000742"
     CDS             complement(42360..43220)
                     /locus_tag="PC621_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4107928.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(43571..44758)
                     /locus_tag="PC621_000743"
     CDS             complement(43571..44758)
                     /locus_tag="PC621_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4107929.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(44772..46163)
                     /locus_tag="PC621_000744"
     CDS             complement(44772..46163)
                     /locus_tag="PC621_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF4107930.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(46758..48458)
                     /locus_tag="PC621_000745"
     CDS             complement(46758..48458)
                     /locus_tag="PC621_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4107931.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(48430..49833)
                     /locus_tag="PC621_000746"
     CDS             complement(48430..49833)
                     /locus_tag="PC621_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4107932.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(50097..50588)
                     /locus_tag="PC621_000747"
     CDS             complement(50097..50588)
                     /locus_tag="PC621_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4107933.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(50614..51426)
                     /locus_tag="PC621_000748"
     CDS             complement(50614..51426)
                     /locus_tag="PC621_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF4107934.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(51439..52590)
                     /locus_tag="PC621_000749"
     CDS             complement(51439..52590)
                     /locus_tag="PC621_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF4107935.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            52860..53501
                     /locus_tag="PC621_000750"
     CDS             52860..53501
                     /locus_tag="PC621_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF4107936.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            53527..55071
                     /locus_tag="PC621_000751"
     CDS             53527..55071
                     /locus_tag="PC621_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4107937.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(55288..55914)
                     /locus_tag="PC621_000752"
     CDS             complement(55288..55914)
                     /locus_tag="PC621_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF4107938.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(56049..56765)
                     /locus_tag="PC621_000753"
     CDS             complement(56049..56765)
                     /locus_tag="PC621_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF4107939.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            57180..58394
                     /locus_tag="PC621_000754"
     CDS             57180..58394
                     /locus_tag="PC621_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF4107940.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            59013..>59237
                     /locus_tag="PC621_000755"
     CDS             59013..>59237
                     /locus_tag="PC621_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107941.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKP"
BASE COUNT        19314 a        10563 c         6399 g        22961 t
ORIGIN      
        1 ctataaatct atatcaagag gtgctatttt atttttaata taattaatat tctattacgc
       61 aaaaaatgca tatatttcat tattttatgg aaaatattac ttcatttaac tctatatcaa
      121 actcattaag taaaaatcta attatattta atttagcctc tgtatttaaa tttgtttcta
      181 aatatatatt ttctgatatt ttaatacctt ttcttaattt atatacatct ctagacttaa
      241 tattatctat aaaatctctt ttataagtct tgtctacaat actgttaaac cttacagaat
      301 ccaaatcata taaagtaaga gcaacttgtt cataaaattc tgtccaattt ttagccttat
      361 atgactctcc taatatacta aatgatacta tactttcatt tgtaaaatta acatcatcat
      421 cctctaacgt aaatatattt tcactgttac ttggaggttc atatgtaaca cttggatatt
      481 tccaaatttc tacagctcta tctagtaata tcttagttct attttttatt tcttcttcat
      541 tccatttatc tatagatgaa atatatttat ttagataaag cctactatct ttaaatccat
      601 cttccatttc tttcttttct tcaaatgttt tattacttag ttttgaatta tatcctgtca
      661 aagttaaatt tccaattgta tgtaaatatt tatcatgtat ctccttatag ttatctccta
      721 gtgattcctt ccatttattt gttaatgtct gtggcattat atgctctata tttagcatat
      781 tattattgat taaattttct aaatcaacaa tttctttatt attataattc tctaacctct
      841 caagtatata taacttattc tttgacttag tattatatat atctttagtt tcaaaagcat
      901 ttgaaaattc actgtcagta ggaaatcttt gagatgattt cttatttata aaaatatatc
      961 ttaatatatt agaatagttt tctttataat cactaaattt tttaatttcc cttcctaagt
     1021 tcataaatac tttattcagt gcattcggag gtacatcaca tactattctt cttaatataa
     1081 aacattctac taaattaagg gattctacaa gtactttttt atttattata ccttcattat
     1141 aatcattaaa taattctaat ataaacggat atgatacaaa tatttcaagt ttgtttatcc
     1201 ttttcaaaac tttgtttaca tcattatcat cagtacatga ataagatatc tctctataat
     1261 agttagaaaa tttaagtaaa tcttttaata aaatttctat atctatacca ttatctgata
     1321 catacctttt aaaactaaaa tatactttat ttttattcgg tatacttctc tcctttatag
     1381 tcaaatagtc tcttataaag ctacttacgt tgctacttgt atttttttca attttatacc
     1441 aataattatt ataaaattcc tcttgtaact tagagcttct acccattaac acaaagtttc
     1501 ttactctatc agcttcactt aaatctaagc cagttgaatt tagactttca aagattaatt
     1561 gagggtcatc ttcaccattt tttaactcta tttctactat agttaatttc tgtatagcat
     1621 caaacaaatc atctattgat atattatctt cttttattct attataaaaa tataaataat
     1681 ttgaaactat attagaattt ctatctgtat tcatttcttc ttcaaatagt tcatagtaag
     1741 cttttctatc ttctgctatt ggttttaact ttatttttcc atctttatct cttgagtgct
     1801 tgtttataag ataacactcc ttaatctgtt cttctgttgt aataattcct tttgattctc
     1861 caaactcttt tattatttga cacatggcaa tcaataatat tgacacagta gtcactcttt
     1921 gttgtccatc tattattaaa tattcttttc ccatactata tatagataca actgacccta
     1981 agaaatgagt tctaaatcca gaatctatta tatcctctaa atcatcaaac aatcttttac
     2041 attgttcttt cttccagtta taatttcttt gatatacagg cactacaaaa cttttgtttg
     2101 acccctctaa ataactaaga aattcaattt ctcctgcttt catattaact ccccctataa
     2161 gttaaataaa ttccattcat aactccatgt atctatatat tatatcaaaa ttttgactca
     2221 taaatgtata tgaaagcctt attatatacc tttttatacc tatttttatt ttattaaagt
     2281 aaatacatga ttattctata aaatatacaa atacttaatt ttaaaaatta ttatttaatc
     2341 ttcttcatga gtaattcata gttatttatc aattaaaaag agatattgca taattattca
     2401 atacctctct ttgtatattt acattatcca tatctagtta tctatttcaa cccaatatta
     2461 gactacctac taataaattt attctctaca taattaacaa taattttgca ctaaataaca
     2521 tcattagcct atctctgatg cattttcttt aattttattt atagttttaa tcaatttatt
     2581 tctctcttca atatgatttt caaataaatc aattatactt ccaacagttc taaatgggtc
     2641 ttccatcaac actttgttat cctctataaa tccattcttg actacataat cgactataag
     2701 tttaacaaaa tgtatttgct tagtattaaa actcttatta tttaaaaact ctgaaaaagc
     2761 ttcatttgca gcttctctat ccaatcccac tagttttctc actaattgac ttactggagt
     2821 atctccaaac tcctttttat aatcatcaca attacctaac tgctttaaga gtatttcctc
     2881 caacgtttca acatcttgct ttgttatctt tttattattt cttaatttaa atattgctaa
     2941 ttcatcttta tggctattta aataatactc taccttcttt ttataattct taagattgtt
     3001 tgcattatat acagaatcat ttctatcttc tgctacaatc atatcttcaa aacttgtata
     3061 atatatcttt tgactagact tttcaagata cttaataagc tctcttaatg atatacgtac
     3121 ttcttctacc tcaaatatat ctgaatcttc ccaaaaatcc tctgtcattg ctttttcaat
     3181 agtatatttt tgttgctgga tctgtggtat agtcccaagt ttcgaaagtt tttctgctgt
     3241 aaccattaca tgccttatcc ctttacttgc attattattt tgtaagtatg caagttgtat
     3301 attatacata actacatcaa atcttttagc caattcatca tcactatatg aagttatgag
     3361 tggtgatata tattctctta tctcattttg tgctactgct ccaatattgc tccaaacatt
     3421 ttcattttta tacttatgta cataacttaa attcatccta actatataac tatcctcatt
     3481 taaagctttt acagaagtta taagttcttc taacaattct tttctaagtt ctgcatactt
     3541 accttctata tatttaatat cttgaagttc tttaaccata cttaccttta tattaaataa
     3601 tttttcagta agtgtttctg ctttatttcc ctcaaatcct ttaggattaa atttaaaaaa
     3661 ttcaaaatta ttacaaaaat caaatattaa gaatctttcc ttatccatat caacacctaa
     3721 taaatctgga cataatctag ttcctctacc tatcatttgc caaaacttag tcttacttct
     3781 cactttcttg aaaaaaacaa gatttaggat ttcaggtatg tcaattcctg tatcaagcat
     3841 gtctactgaa actgctattt gaggtaattt attcttatca gaaaaatcat ctattataga
     3901 atctacataa tttatactat agtcaactgc ttttacaaag ttcccaccat actttggata
     3961 cagtttatta aatctttcaa ctattgccct agcatgcttt gtattctttg caaaaattat
     4021 agtcttacca atcttttctt ctccttctat tttcaacccc tctgtcatta gcttatttaa
     4081 aactaaatct attgtgtcag aattaaatag ccactcattt actgcattat tccctatttc
     4141 gtctccaata ctctcatcat cattaaatgt attttcatat tcttccttct cttcatcaga
     4201 aagctcatca tacttaattc catcttccat aatcttagtc ttaaactcaa tactagtata
     4261 atctactaaa tatccatcct cgactgcttt attatactca taagcatatg taggtactcc
     4321 attttccatg tcaaaaactg aataagtatt tttatctatc tcatcctttg gtgtagcagt
     4381 aagacccatt aagtaagaat caaaataatc aaagattgac ttatactttt tataaatact
     4441 tctatgcgat tcatctatta taattaaatc aaaatgacca ggtgtaaaca atctctttcc
     4501 atccttagac ttcgtatcat caattgcatt catcattgtt ggatatgtag aaaatatcat
     4561 cctagcatct tctgggctgt ctttgctatc caataaatta cataacgcca agttaggtaa
     4621 tagcttagta aaactttttt tagcttgctt taccaatgct tttctatctg ctagaaataa
     4681 tatattttta atccaattat gtcttgaaag tacatcaact atagatatag caattcttgt
     4741 ttttcctgta ccagtagcac ataccaaaag agttttgcgt tgcttatttt caagagcctc
     4801 acatacagcc ctaatagctt ctttttgata atatctattg gatatttcat catttattgt
     4861 tacagaactt aaagacttct tactctttct tctatctatc ataagttgta attctgattt
     4921 tttataaaat ccataaactt ttctatctga atagtcatcc caaatataca tatcaaaacc
     4981 attcgtcaaa aatattactg gtctttgact atatctcttt tccaaacaat ctgcatataa
     5041 tttagcttgt tgttgaccaa ttttcgggtc tttgctcgtt cttttcgcct caactactgc
     5101 taatggctta ccattttcac cataaagaac ataatctaca tatcctacac cactttcatt
     5161 aggcatacct tgtacttcta tttcctcccc tatatctttt ccaaattccc aacctgcaag
     5221 ctttaattct atatttatat atctttttct tgtctcaaat tcgtttactt cgtctatctt
     5281 gaaatcgtag tttacagtat taacttttct cttttcagtt agtgactttc ttaatttctc
     5341 attttctttt attacttctt caagcttttt atcttttgaa cttaatctct cataaagatt
     5401 tttaagctcg tctggtctcg tccttttctc ttccccatga agtaataaat cttcattaaa
     5461 gtcttctgct gtatattcat ctgaataaca atagtctatc caactaacaa attgatgcaa
     5521 attgttaagt gaaagtattg cttctcctct ctctatgttc gcactagtat gtacagataa
     5581 attacctaat ttaactatat attttaataa aggtaacata tcatagtcta taagttctat
     5641 aaaactatta ttatgaatca acgtactaag attatcttga taaggcaata ctaaatcact
     5701 atcattagca tatagccatt ttactgcaag ctctaaagct cttcttgtta tagttgcaca
     5761 agtagatggg ctaactagta tacttttttc tgcttctaag caagcagtat taaaactatt
     5821 gaaatttttc ttcttttgta aaaagtcaaa attacccaag gcttttctcc cccttttgtc
     5881 ccttagttaa aataaatctc tctaaaaact atttcttaca gctgtttctt atactaacaa
     5941 atacttcttc attttctgta aacaattcgt acatatcttc aaactgtggt atatcatatc
     6001 catgagaatc atcatttctc aattttctaa gtgtttctat aactatagaa gaatttttag
     6061 gtaataaatt gtgatactca caagctaaaa ggtagtcata aaagttttta gattttatgt
     6121 ttaaatcaat tgatttataa tacaacattg cttttgttaa ttctaaaaat gagaccatat
     6181 agcttttaaa accttctttt atgtatctag cccataactc aggcatttct tccttatcat
     6241 ataagatttt gaagttttgc aggtcatata agcaagaacc aaaatcccta tttacttctt
     6301 ctattgagtt ccaagataat ttgtttctaa ttaccataca tgtccctcca atctaagata
     6361 tctattacat cactgttttc aaaatagaat ttatttatat aatccttaaa atctttagat
     6421 tcataaataa cttcaccttc taaaatagct tttttaagtg ctattgtata attatctgta
     6481 acaattttta ggtctacatc aatgtctaat atatttctta gagcttctgt cttagttgca
     6541 agttctgtaa ttggtacttt tttgatagga atccaagcta tgtctatatc gctttctgat
     6601 gtatgtttat ctgttccata actaccaaat attatgattg ctagagtatt ttcttttgga
     6661 aatacattta ttattgagtt tttaatctca tttggtatat ttctcattta atcacctctt
     6721 gtttattagg taatattgct taattgaatt tatatccata atcttttaat ctatttatac
     6781 atatttctct ttcttcatca gtaaacaact ctttatactt ttcttttaat actaatactt
     6841 ctaatgataa atctaatctc ttacactccc atagccttgt aaatccttca gtagctcctt
     6901 ctttattgat taattcttta gcagctaata aggctccttt gttatttatc agttgtaaaa
     6961 atcttgttgg cttatagtta cattgtaaat cagcttcttt atagatattt accatgtcat
     7021 gatataaatc tagctctatt tgattcatag attttattcc cccattttaa tacttgtatt
     7081 tcagttgaac aattctcctt taaaagcttt ttgcattaat gagttaaagt tatcttctag
     7141 ttcttttaag ctagtttcca tttcaaactt caaagagttt acttgtttaa cgaagttggc
     7201 aaattgattt tgaagtctaa taggaggtac aataatattt atttttctaa cagcatcttt
     7261 agttatcgtt tttgttacag ctcctcttac tttctctttt attttacttt ccatatagga
     7321 tgttagtaaa aggttatata agaatatatg attcacttta tcttcattaa atctaagcat
     7381 aaattgattc atagctaaag atactggttt atttaaaaat ggcattaaat aaactttacc
     7441 tgcactacct atcttattta ttataacctc accaccaaac acctttgatt tttcaagata
     7501 attatatgca tgttcatcga tatacttaac acctttttca aagttattat tttctaaatc
     7561 tgtagttctt accattaagg cataaccttt tgtatctaaa agagttacat tatctcttaa
     7621 ggtttcataa ctaccatttg aatgataatc agttaaaaca tctaaatatt tccctatttc
     7681 tgaaatttcc cagttttttt cgttctttga tggggtacca aacatctcaa taaatcttga
     7741 tttaactaat tcatccaata cttctatttg ctcttttctt ttatctatta actcttgagc
     7801 cttgtctaaa acacgcacta ccttttcttg cacttttaaa ttatacatgc gtatttttat
     7861 attagctaat ccttcattat ttaaagctga ttgtgtagca cctttactta acctattttt
     7921 ttgatttaga aaataactac tatttaagta atggtataaa tattgaggca atatttcttt
     7981 tgatggtttt aaaacataaa agccagtcga tactattata tttttattag ttttattaat
     8041 aactagtgtt ttctttgtgt ccttcatttt tgcaaatata acttctccta tatttacagt
     8101 ttgatttgct ctagaaggtt tattttcata agtaactatt tctagattgc ttatcttgtt
     8161 aaaatctaag tttccagtac taagatatga gcgttctcca tcaaactttt ttacattttc
     8221 cctgcttata ctaacaacat ctaaaatttt tgcttcatca accatactct acacctctaa
     8281 cattctttct agttcctcta atccatccac tatctctttc tctaacacct taaccctacc
     8341 tataataaca ctaggagcat cataaactac ttcctcatac actacctctt tatacttatt
     8401 aatagataaa tcatacccat tctctcttat ctcttccaca tcaacaaaaa agctttgctc
     8461 tgttcttttt ctatctactt ccccatcaag attatcaaat ctcttaataa tatcctctat
     8521 atcactatcc tttatctcat ttctcttatc atccaaagaa taaccatcag ctttcatatc
     8581 atagaaccat accttatcag ttccacctgt ccctgtttta gtaaatatca ttatagctgt
     8641 agatacccct gcatatggct taaatacacc acttggcatt gatattatag cttccaattt
     8701 atgattatca actatttctt ttcttatcga tttatgtcca cctgttgaac caaacaatac
     8761 tccatctggc actatacatg cgcatcttcc accaattttt aaaactctta aaaaaagtgc
     8821 caagaataat aactctgttt tctttgtctt agtcactttt aataaatctg ctgacactgc
     8881 ttcataatct aaacttccct taaaaggagg atttgctagc accaatgtaa acttatcttt
     8941 atctgtatta acctccgata aagaatcctt atactctata ttaggattgt caacgccatg
     9001 caacatcata ttcatagcac ctattctaag catagttcta tccatatcaa atccatagaa
     9061 cataccatta ttaaaatgct ccttcagccc ttgcactaag aaaagacttg aatgattatc
     9121 ccttaagtac tgttgtgatg atactaaaaa acctgccgaa cctgctgccg ggtctactat
     9181 aatatcttct ggtgttggtt taataagtct tactatcata tctattatgt gtcttggagt
     9241 cctaaattga ccattagtac ctgctgtagc cactttggat aataaatact catacaaatc
     9301 tccctttgta tctctatcct tacttaattc taaattacta ataccatcaa ctattttaga
     9361 taacataaga ggcgtaggaa ttttaaatat tgcatctccc atgtactttg cataagctga
     9421 ttctttattt ccatgtaatt tttttatgaa aggaaacact tcattttgta ctatttcata
     9481 cattttttct gagtttcctt cattagaaaa cttactccat cttaaatgtt gatgttcatc
     9541 atcaaatatc ttttcaaaag aaacacccaa aagagttgct tctgcttcat tttttgtttc
     9601 tacttcatcc aatcctttaa taaataaaag atatgtaaat tgttctataa cttctaatgg
     9661 attagttaat ccaccagtcc aaaatgtctc ccaaatcttg tctactttac tccttaattc
     9721 tcctgtaatc atatatgact ttccctccag tgttcataaa ataatttaaa tatatatttt
     9781 agtcgtacgc cttaatagtt agtgtcttat gttaaatcca agtttttact acacaattat
     9841 taaattattt ttcccattta catattttag ctaaaaaact aatgcaatac tcttatttta
     9901 tgattattat atataccata caaaattatt ttatcatata tccatcatag aagtcatcta
     9961 cctggataac tcttaccata ttataacttt ttaatattaa tttaaactta gaaagaaatt
    10021 ttttatttaa ttatatcatc ttccttattt caaatttctc aactacaatc ctaatcaaaa
    10081 aataaaataa gatgctgtct caaaatagat ataatcttct tttttattgt cagaatgatg
    10141 aaaatatatt tgatttcaca caaaaaagag tgcctcatga actaaaaagt tcattttgag
    10201 acacccctta atttaatctt acttattctt tccctttatt attaacactt cactgtccct
    10261 attgcttttc ctacacatcc acaaacttcc atcctactct taacaaaatc tttaactgac
    10321 tctaaacctt ttttaccata tttcttaggg tcaattacct ctgggtcaga attaagcaca
    10381 tccttaaccc cattgctata agcaatcctt aattcagttg cataattaac tttgcaaata
    10441 cctcttttta tagattcctt aataacctca tctggtattc cagacccacc atgtaatact
    10501 aatggaactg atacaacctc tctaatttca gataatctat ctaaatcaag ctttggctct
    10561 cccttatata gtccatgtgc cgtacctatt gctactgcca aagatgatac ccctgttctc
    10621 tctacaaact cttttgcttc taatgggtct gtatatgctc caccatcacc accatctaaa
    10681 tcgtcttcct tacctccaac tttgcctaat tcagcctcaa caggtatatc tgaaggcata
    10741 catgcatcta caacagattt agatattgaa atattatctt cgtaactttc atgagaacca
    10801 tcaatcataa tagaagtata tccaactcta agtgcctgca tagctagtcc aaagcttgaa
    10861 ccatggtcta agtgcattgc cactggaaca cttgccttct ctgatgctac ctttacattc
    10921 gctaaataat aatctagccc tgcatacttt attgttgaag gtgttgtttg caatattact
    10981 ggtgatttaa gttcttcagc agcctctatt actgccataa ccatctccat attttcaaca
    11041 ttaaatgcac ctactgcata atgcccttct tgtgcatcta ataatatttg tttagttgtc
    11101 actaatgcca taatttctcc tcctatatgt ttatactgat ttgatttctt cgttatttaa
    11161 ttttgatact atttctctac ttgttttgct atttaataac tcatttaaaa attcttcatt
    11221 cattaatttt gaagatagtt gagataatgt ttttaagtgt aaattatcac cattatctgt
    11281 agctgatatc ataaatataa tatgtgccaa gcttccatct tctgattcaa agtcaaaccc
    11341 ttcttttgat acacctactg ctacacgtgg tacctttact gcatctgttt ttgcatgtgg
    11401 tatcgctatc cccataccaa aacctgtaga tgatatttct tctcttttaa ggatttcttc
    11461 tttaaacttc tttttatcta aaagaacacc agatttatca agtattccaa tcatttcatc
    11521 aattacatct gttttatttt ttcccttaac atctaaaatt actgtttctt cgctatataa
    11581 agagatatta ttagatacgt tttccgattc tgcactttca tttttatgtc catcttttgt
    11641 tgcatttgta ttttctatag tatttttctc atttactact ttaggtgtaa ccatcatata
    11701 aagtacagca cctactaatg aacctacaaa tatacaccct acccataaaa gtggcttatt
    11761 gactaatcct agaaccaaaa accctccatg tggagctggt acttctattt tcattatata
    11821 agttaaaatt gatgatattg atgatgcaat cataagtact ggcaacactt ttaatggatt
    11881 ttttgctgca aaaggtattg ccccctctgt tatatgagta ctacctagta tataattgat
    11941 taatcctgct gctctatctt cttctgtaaa tctattttta aataaagtag atgctattgc
    12001 tattaccaat ggtggtgtta tacaagctgc agatacacct gccataaata aataatttcc
    12061 ctgtcctaga agcatagttc cagttacata tgctgcttta tttactggac ctcccatatc
    12121 aaatgctgac atacatccta ttactattcc caaaattata ggacttgaat tttgtaaccc
    12181 cgatagaaag ttcatcatag cattgttaag tgctgaacat ggtccaccaa gtaacaacat
    12241 tgctacaccc acaagaccta cgctgacaat aggaattata aaaatcgatt taagaccttc
    12301 taattgtcgt ggtaattttc ttaacaaatg tacaaatagt ttaactaagt aacctgctaa
    12361 aaatcctgat atgattccac ctaaaaaccc cgaacctgta tcaaatgata tcattcctcc
    12421 aacaagtcct gccaacatac cagctcttcc cgatatagat tgagctataa atgctgccaa
    12481 tacaggaacc atcaagttca tagaatatcc accaacagat tttaatgtag ctgctatttg
    12541 attatattgt tctgaatttg ggtcaaatga ataaatcccc cataaaaatg atatagcagt
    12601 aagtactcca cctgctacta ctaaaggtaa catgtgagat acaccattca ttaagtcttt
    12661 atacaacttt tgaccaaacg ataaatttcc ttcttctgca attttactgc ttgataagtt
    12721 tccttttcta atagctactt ttccatcaat acacctttgt ataagttcat tagccttatg
    12781 tataccttct ttgacaggta cttctattac cggctttcca ttgaatctgt ccatatcaac
    12841 atttttatca caagcaacta ttattgcatc tgccatttcg atatcttttg ctgttaactt
    12901 attttctaca cctatagctc cattcgtctc aacttttatg tcacacccta attcccttgc
    12961 tgaatttttt aatgcttctt ctgccataaa agtatgagct attccagttg gacaacctgt
    13021 tacccctaga atcattttct ttcccatctt tataccacct ttctgactct tatatttttt
    13081 atataatctt ccacattctc caaattacct ataccattac tttcagccac acttgctcct
    13141 gttgctgctg acctttttaa tgcttcttca atatgttctg gattttccaa ccatatactt
    13201 aaaaaagcag ctaatgcaga atcaccagca catgcagagc tgactactgt aactggttga
    13261 gcactagcat aataaattga ttttccatta taaaaataag aacctttttc tcctaaagtt
    13321 agcaatatat tttgagcacc ttttaaatgc agtaatttaa gtacatcctt aatatcttct
    13381 tcatctctta caataattcc aaatatatct ttgatttctt catcatttgg ttttatcaaa
    13441 tatggattat actgtagtaa ctcctttaat tttggtgaac ttatatcgag tattgtcttt
    13501 atacttttat ttttacaaat tttaaatata tcctcatagt aattatcatc aataccagga
    13561 ggtagacttc cacttattga taagtaactt atatctgcta atgactgtat cttatctagc
    13621 atatctagtt tttgtttttc gtttacatat gacccagagt ttacaaattt aaattcatca
    13681 cttccatcat ttaaaaatat atttatacga gttgtatctt ctacccaagt aggtaatacg
    13741 tcaaatcctc tcttttcgct ttcttcaact atatattttc ctgaaaatcc tccaaaaaat
    13801 cctaatatct tagattctat accataatga cctagtacaa aagttacatt taatcccttt
    13861 ccattaggag aatatacagc atttgatgta cgatttacta attttctctt tatcccatta
    13921 gtagatatat tcatatctat tgcaggattc gtagttaatg tatatatcat tgtcttcccc
    13981 cttgcttgtg ctgtgtatgt tttcattata ataaaaaagt ctatgaaaat cttttcacaa
    14041 atagaaaatc gattttcata gacttttcaa tttaattttc agaattatta tatctgacca
    14101 tataatctat aattatccta gatataatat acaagggcaa tctggaagat acttcatatt
    14161 cagaaattga tatatgttca tgtttgtatc ctacaatata taaatccgaa tactttataa
    14221 gttttgcact ttcactacaa cagcaagtta taaccttagc tccaactaaa ttggcattct
    14281 gtgctgattc tacaagctct atggtatttc cattcaatga aaatatgatt ataagttcgt
    14341 ctttttttat tgatttagat tttattttca ttatgttggg gtcatttata aaaacaacat
    14401 taaaatcaag taattgcaat ttaagactaa actcttttgc tacatattca gtaagccctc
    14461 ttgccaaaat atatatctta ctagatttat ttatagtttt tattatattg attagtgtag
    14521 ttaaagatat ttgttctata actctctgtg cttctataaa ggatttattt attatatccc
    14581 ccatattttg tatatcatca ttttcatctt ttactgttaa tctataccta agttcattga
    14641 atccatttaa accacatttt ctaattgctc ttgaaacggt tgctggagat gaatatgttt
    14701 catgagcaat ctctactata gataattctg gtaatattga ttcattttca tttataaatt
    14761 taattatttc aagttccgtc ttcgttagat tttcagaatt acttttgttt agctcaatta
    14821 tcatatgtta tctcccctta tataagtttt atttgtctaa atttatcgag taatataata
    14881 tattacctct taactactta tttttatata caattaagat ttagtcaatg tctatatatg
    14941 taaatttata atataataat tttatgcttc aattaattat gtaattatta gagtattttg
    15001 taataaaaat tggagttact caattactga aactgagtaa ctccaattct tacagcctca
    15061 aattaactcg aattattgaa aatgattcat aaacattatc ctatactctc acttatcaat
    15121 ttatatactt gagtaatacc tttttttatt aacagaaatc atcttactaa caattgaaat
    15181 tgtagttcca gtacataata ttgaaaatat agttccaaac cctaaatttc cgcctaaata
    15241 tacacctaaa attaaaaata ttaaatctgc tatagttttt aaaactttat atggatactt
    15301 aaatttgccc tcaatataga ttaatatagc atctaaaggg ctcattccaa tgtcaaattt
    15361 tataagcatt gcaaatccac tacacataag tatatgtcca attataaagc tcattattct
    15421 aatgataatc cctaaactac ttatattaaa atctaataat aaatatatat acaaatctat
    15481 aaatattcct gctactaata cataaacaca gcttcctaat cccaaacttt ttttattcaa
    15541 taacaatgct ataagtaata gagttccatt atatattagc cctgctattg tataacttat
    15601 acctaacttt atatgtatac cctcatttaa aagtgtaatt gcatcactac caaggcttga
    15661 ctgaagtata agagatatac cacatcccag tatatttata attaaaatta caaatataaa
    15721 tttttttatg acattcataa ttaataaacc accttattta taattataat ccctcttata
    15781 aaactgtatt aataacatag tatttataag agggattatt tatatttaaa aactatctac
    15841 tttttctttt aattcttctc cattagttct tattacattt ttataccaat aaaaactatc
    15901 ttttttgtaa cgttctttgc ttccatttcc attgttatct atatctacat atataaatcc
    15961 atatcttttt tccatttctt gagatgaaca acttactata tctattggcg accacataca
    16021 ataagctaat atttcggttc catcagctat agcttcacct atttgttcta tatgttttcc
    16081 taaataatct attctataac tatcatgaac ttttccatct tcaaatttat catgcattcc
    16141 aaatccattt tcagctatca ttattggctt ttgatatcta tcataatatt gagataaagt
    16201 attgtataat cctttagggt caattgccca cccccaagga tttgctttta agtattgatt
    16261 ttttacatta tcaattggat tcatggtatt tttacttgct gatactgttg tagagtaata
    16321 gtatgataga gctagaaaat ccatagtatt ctcttttaac actttatcat caccttcttc
    16381 cataactata tttatgttat tttctttgaa gaatctctta atataagctg gatactcccc
    16441 tctaaaactt acatcagtaa aaaaatattg tattctattt ctttttaaag caaatactac
    16501 attttcaggg tcgcaatctt ctggataaga tgtacaatct gccatcatca tccctatcat
    16561 catatctgaa tttatttctt ttccaagctt cttagttaaa gctgtagcca tgaattgatg
    16621 atgaatagct tgatatttgg cctcttctac atcatctacc tggtctacac aaaatgcaac
    16681 tgaattaaat gattcatgat acattaaatt tatttggttt ataactatcc aatatttaac
    16741 tttatctttg tatctattta atattaattc tgcatatcta acaaagaaat ccactacttt
    16801 tctattagac catcctttat attttaatac taaattcaaa ggagtctcat aatggagtag
    16861 tgttacaata ggttccatac cattatctat tacttcattt ataagattat cataaaactt
    16921 taatccctct tcattaggct ctaaatcatc tccatttgga aatattctag cccaatttat
    16981 tgaaaatcta aaacacttta accctagttc tttcaaaagc tttaaatctt ctttgtatgt
    17041 atgataaaag tctattccat tacgcttagg ataataccca ttattatctt tcatagcaaa
    17101 ctttatttta tctacagtca tatcagactc gtaatgttta gatatatctc tatctttatc
    17161 atacatatgt acatcagcta agcaccaccc tttgccacct tcattccaag caccttctaa
    17221 ttgattagct gctacagctc caccccacaa aaaatcttta ggaaacttac tcaatttaat
    17281 cactccttat tttatttatt taaatcattt actattattt accttatatt ctatagactt
    17341 tactattcct actaaagaca tagttatatt taactcgctg aatattgtca taagagtatc
    17401 ttgagcatgt gcaaataaaa gtgaatgtgc tcttttttct ccactagctt ccccttgtat
    17461 aatgttagtt tgagctctat gtgcttctgt cataaactct tttgctttag ctagttttaa
    17521 atcagcattt ttataatcaa acctttctaa atcattaaga gcatgtttaa tttctgttct
    17581 agcctcacct gcacgaagta ttatttgcat tgctacttct actaattctt catctattac
    17641 atgttcttca gttgtcatat ttataatttc ctttcaacat ctaaatttaa gcttcttgac
    17701 tctctaattt tagttcttct ttttcataag ctttaaaaaa tggataccat atcatgaata
    17761 gcaatactat atttattgct aaaacaataa gccctctaac atcttgattt accataaaag
    17821 tagatatacc aaaaggaaga taccataaat taaacacctt actcggaatt gtcacaaatc
    17881 ccatactcat tgataagtaa gtagttattg atattactac tgttcctatc cacataggta
    17941 acattaaaaa tgggttccat gctactggag ctccaaacat tactggttca tttatgttca
    18001 ttatagaagg aacgatacag gctcttccga ttgcttttat tcttccagct ctagataaac
    18061 acatcattag cactagtggt aatgttgctc cccacccacc taaagttatc caacctgttt
    18121 gaacaacttc ataagtatat atattagttg caagccctcc tgatgctaca gctgctgcat
    18181 tagctgcaat tgcactaagc tgaattgcat aagttattgg ggataatacc catccactta
    18241 tacctaaaga atataaaaat actactaaga agtttaatag tacaaatcca ggcaatgttt
    18301 gtgctatatc tgatactggt ttaaatatcc taactattat ttcaaacata tcaaagttaa
    18361 gtccataaat tagtccccaa cctataaaca ttattactgc tataggtaac atactatcaa
    18421 accacttact tacaaaatca ggaagtgctg aatcttcacc aaaaaatgag aaagacccaa
    18481 ataaagacat tattaatccg gagaataatc ctgcaactag tgctacaagc attccttctc
    18541 ctccaaatct attgaagtta aatatagctc cttcattcgt aatctctgga cgaatcatca
    18601 ttaacattaa tccagcacct gcaagaccag ctactacact catactttca aatttcttat
    18661 tcttcattat ttccataggt aataaaaatg ctataaatag tcctaaaagt ccaaatgtat
    18721 attgatttat tggacttaaa tcaggtaaga aacttatgta attcttcaag acagaaacta
    18781 tagttactaa agaacctaca aataccatag gcaaaacttt cattatagtt gattgaactg
    18841 attgtaccca tatattctta gttatcttat ttacaacagg agtaaacttt tcacttaaca
    18901 aatctataaa tttattcata aacaatctcc ccatagatat tttatttgtt taatattaat
    18961 tctaatgctt cttctaaagc ctttacacca tctaatcttc catatgctgt tttagatata
    19021 catgctactg gaacattata ttcatcagct tttttttgta tttcatcttt catataatct
    19081 aaatgtggtc ctaataataa acaatctatt tctggtaaat atttatctat ctctgactca
    19141 cttctagctt ttatatctgc ttctatgcct tttttctttg cactctttct catactagtt
    19201 gccataaaac ctgtacttgc tcctgagcca catgccaaca acacttttat attcttcata
    19261 acaatacctc cagtattttt attcattaat ttatttctta atgaattttt atttgatttg
    19321 attgtattta tattgtactt cctaaataat ttcctttcac ataaatatat ttcctaataa
    19381 taggaaatca tttactctta accagacaca taaatatata gtataatgta cattataata
    19441 cgtacattca attatataaa tatcatatca tacagatttt attttaaaac tatgagaggg
    19501 aatctaatga aaacatctaa aaaagagtta tttttaaact tcataaaaac gcaacatgat
    19561 tggatagatt catccacatt agctaattat cttaatgtat caacacgttc gattagaaaa
    19621 tatgtaaatg aaattaattc tactggtgaa tttatattat cctctaaaaa aggatataaa
    19681 gtaaatctca ataataactg tcaaaccaag gttgatagtt cagaaaatat atcacctgat
    19741 aacaggttaa atttaatttt aaaagaactt atagttaata gtaatggaat taatatattt
    19801 gatttaagtg aagaattgtt tgttagtcca gctactattg aaggggatat agtcaaagct
    19861 aataaattta taggaagtta taatttaaag ataaagcaat cgaaattttt attgaaacta
    19921 attggaaatg agtctgcaaa gcgcaaatta atgagctcta taatatttaa agaaacggga
    19981 agtgattttt taagcttatt tgatgtacaa aaaatatatc aagagtataa tttgaccaaa
    20041 ctgaaagaaa atataatata tatattaaaa aaatacaatt tgtttataaa tgagtacgct
    20101 ataaataaca tacttcttca tttaatgata actatagaca gaataaaaaa gaataactac
    20161 atagaatctg tcgaagtagt aaactacata gataataata atgatataag catttcaaga
    20221 gatatatcaa attttttaga gtctgaatac aatttaactt taactagtgc agaattatac
    20281 tacttagtat ttcagttaac aaacaaaacc acagtcttaa attatcatga aatggatact
    20341 aagtcattgc caaattatat agatgaacat tttgttaaat taactaaaaa aataattaaa
    20401 aatgtatatg acttatactt tatagattta tctgatgatg aatttgttgt aaaatttact
    20461 cttcatgtaa aaaatttaat cagtagggct aaaaataatc aagttttgag aaatcaaata
    20521 cctcagaagt taaaagattc ctaccctctg atatatgata tatctgttta tatttgtaat
    20581 caaattcaaa ttttagaaaa catagaaata gatgaagatg aaatatcgta tatatctctt
    20641 catgtaggtt cattttttga tagacaaaaa ttactagaag ataaaattct ctgtgcatta
    20701 attactccaa attactatga tttacaattc aaaatagtaa gagacctcga gaagagattt
    20761 aatgaatcca tagaaattat tcaaattttt tcagatactc ataatcttga ttttgacaat
    20821 aaggtagaca tggttattac tactttacca attagtgata gatgccctat accttttgtt
    20881 tatgtaaatc cttacctaaa cagaaaagat tatgataata tccaaagtaa atttattcag
    20941 attaaagata gaaaaaatat acttactgta caaaatcatt tagaaatgta ctttagtgaa
    21001 tctttgttta tgaaaaacat atatttagat agtgctaaag attacataaa atttatggga
    21061 aatattctgt acaaaaataa atatgtcaaa cccaattata tagatgatgt tcttataaga
    21121 gaaaaaatgt cttcaactgc atttaataac aatgtagcaa ttcctcatag catgaaaatg
    21181 gatgctttaa aaactggagt atgtttaatt gttaacgata aaccagttaa atggggtgag
    21241 gaaaaagttc aaattatagc tatgatacct ataaatgaaa aagaaaaaga aaaattcaat
    21301 tacatatttg aaagctttat tgaaatatta tctgagtgga ataatgttaa agaacttact
    21361 aaggcagata actacagtag ttttatgaat agaatagctt atcttattca aaatatctaa
    21421 atccattaat ttatttccaa tatatgtttc ttaagttatt aacacaaaat agataagctg
    21481 tcacactaaa aagtctaaat taatatgtga cagcttttta cctaatatac tttgctttaa
    21541 tactacatat tttttgattg ttgtatacta gttataagat tgaatatttg tgttgttgta
    21601 gctgtcatat tctcactagc agttgcttta tccattgctt cttctattgt catagctcta
    21661 tttactatag aaaaataagc atctataccc tcttcattac atttaactgc atcttctgtt
    21721 gtggctccag ccaaacctat tacttttacc ccatattttt ttgccagttt tgcaactcct
    21781 attggagcct tgcccatagc agtttgatta tccaatctac cttcaccagt tatgacaaaa
    21841 tctgcatctt ttaactcttc ctctaattta atttcttcta atattatttt tattccagat
    21901 tctaattttg aatttaaaaa tgctaaaaaa gcaaaaccta atcctcctgc tgctcctgct
    21961 ccttctacat tagctacatc tttacttaag taatttttaa ctactttgga aaaatttcta
    22021 agcccattat ctaactcctc aattatttct gatgttgcac ctttttgaga cccatatatg
    22081 tatgcagctc catttttccc aaacagcgga ttatttacat cacaggctat tttaaactta
    22141 cattcatcta attcctttaa tctattttct atttttatat gtcttatttc atttaaaact
    22201 tttccaccta atcctactaa cttgttattt tcatcataaa actcaaaccc taaggcttga
    22261 agcataccta tcccagcatc attagtagca cttccaccta ttccaactat aaaatttcta
    22321 tagcctttat ttattgcttc ctttattatt tccccaacac cataagttgt agtgtataaa
    22381 ggatttctaa gttcagtcgg aaccaatgtt aatcctgatg cttgagctat ttctattatt
    22441 gctgtacttg tattttttaa taatccatat gttgcatcca cttttttagc tattggtcca
    22501 gtaacttcta tagtctcttt tttaccattc atcccttgaa ctaaagcatc tactgtccct
    22561 tcaccaccat cagcaagtgg caatatcttt acttgcgcat cttctttaac cttcaatatc
    22621 ccctttttta ttgcatttcc tgcctctatt gaagataaac ttcctttcaa agaatctata
    22681 gaaattaata ttttcatatt tatctactcc tttttattta ctctttattt taaatatata
    22741 attttataag cttaatttat cttttatttt atactctata tttcactttg tatacaactt
    22801 atacccttaa tattctttta tcttataaaa aagggagtgt ctcaaaatga actttttagt
    22861 tcatgagaca ctctttttgt ataaaattaa atatatgttt attatttcaa caatgataag
    22921 gaggattatc tctattttaa gataattcct ttttagcact atctaatcat ttttagcact
    22981 atttaaccaa tatagtttta acttggttca ttaggcactc tacatttaca gagttattat
    23041 ctattgatat agattctaat tctttttcat ttaggttagt ccatttagct gttttaatat
    23101 ttttgttaaa tactacgtta gctgattttg tttcttcttt agaattgtaa aatctaacta
    23161 ctaatgcctc ttctttttca gcctttttta caacacttaa cacaaggtcc ttattagttt
    23221 ccttaagtaa actatatgaa taatctactt ctatttctga ttgattcaat ttcatagcat
    23281 tatatggtat tttattataa gtttgtattt ttgttaaata ctctttagcc atatttgcaa
    23341 catttgcttc tagtgtagat ttttcatgag tgactattgc taagtctatt tttatatctc
    23401 ctatcatttg agaatctgga gtaggcaact ttattcctga tggacgacct gggcgtcttg
    23461 ccatttcttc tttacctagg aatcctacac ttctaaataa tgttatagct attgtgtcaa
    23521 attttttccc tactatttca aactctcttg tactgtttgt aagaacagct actccatgct
    23581 ctttgtcaga caatccaacg aatgttagca ttggatatat agaatctggt ctttcactcc
    23641 attcttcctg ttgccaaact tccatagcat catcatacac acttctcttt atatgtccaa
    23701 actgattatc agacacagaa aactttgaag ctatatttgt tggtatataa gttctaaccc
    23761 tacaatctct tgcttggtta tttatattga atactacatt tattatagct ttatcatttg
    23821 gaacatttat aactatatca aagtcaataa atgaatctgc tctattattt tttctagctt
    23881 gtatatcttt tggtactttt aatctataat ttatctttat ctcattatta aatctatttt
    23941 tctttatagt ggaatttgct actatattat cgttaaatat taatttctca tttggaagtg
    24001 gtgaaaaatc atactcatct ccttcatctc ctccattttc catcaataat acattttcaa
    24061 aagttttatt taatttttta tcagatatct ttaatgtccc gttttcatta acttgaatag
    24121 tataaaagtc attatctacc atgtcacata cttcataagc tttttctaac atatgttcaa
    24181 cttcttttat catatacgcc ttatacccca ttgcaggtat tttatcttta aagtttattg
    24241 tatataccat aaatgaatca tagtttccat aatgaacaat ttgacggtct attaatcctg
    24301 catctattac ttctttatgt accacttcaa aatccaactt attattatct ttatcaaata
    24361 tttcaaatgc tttcatttta gttataactt gggcgttcac tatttcattc ctattgtaag
    24421 gcattaagtt aaatgctact aacttatcta aagccatttc acaactcatt gcatcaacta
    24481 ttcttctttt gtaaaatgtt attaattggt ctactttttc ctcagctaag aaaaatctat
    24541 tcattatttc ctggtgaact ttatctgaac aacaacatcc aatgctgtca tgagcatgat
    24601 ttttcattat ttctttccaa attaactcta ttaatccatg atgatattca aatcctagag
    24661 aatatgcaat agaagctagt ggctccaata tgttggttat ctttgattct attctagcat
    24721 ttgctgattt tatatccatt cttgttgaga atatacttct atgaaccctc atatatttcc
    24781 catctaaaaa ttcccctttt attgtatcta tatccttatt tttttctagc tctttaaata
    24841 tgttttcata tctacttaag aaaaattttc tctctggata acattctttt aatttatata
    24901 taacttcaaa tatattcttt tgaattggca tttggtcatg tccatttgga agtattatat
    24961 ggtcagtagt tgcaccctta tcaagaaccg gtaaatattt atccattctt gttcttagct
    25021 catcttcatt cattggtaga tattttccta ttgcataacc taatggaagt atttgaacca
    25081 aaacagaaga cccatcatca ctcttccaat taaactctgt tttattagtt ccttttcttt
    25141 cagaacatcc tctccaaaac attgagtatt ttatatcaaa cccatttagt atttgcggca
    25201 tttgagcact ttgaccaaat gaatctggta aatatcctat tttcatatat tctccaaatt
    25261 catcacaatc ttttattcca tacagtaaat ttctaagtat ggattctcca ccaactacca
    25321 tttcatcagt ttgagtatac caaggaccta ttattagcct tccttcttga actaattttt
    25381 ttattctctc cttacattca ggcttaactg ctaaataatc ttctaatata gcagtttgac
    25441 catccatcac ataatatgga tagttatgat tattttctaa catatccatt atttcttcca
    25501 tattatttac aagtaatatt ctagattcct cagttgagaa gtaccattct ctatcccaat
    25561 gcatatgagg aactatgtgt acattttttt tcattgctta tcctccaaaa cttatctgat
    25621 aatattttct gtctaattaa aaatccaata aaaatactac tgagtttaca caatttttta
    25681 atatttattt tatcactata attaaacctt tgatttaaat atctaataac gtatctttta
    25741 atgtatcttt ctttgatttt tttgtttcct agttattatt aataatattg ctgatattat
    25801 agctccaagt actgccgcaa agaaccatat tgatgctata ataaatcctg acatacttcc
    25861 ctctaaaagc gccaatgaga atatccccgc tccaggcact tgaagaccta tattgaagaa
    25921 tgctactata gacccagtta tggctgaacc agctactaat gatggtataa catggattgg
    25981 gtcctctatc ataaatggta ttgcaccttc agttattcca gccaatccta atatccaagt
    26041 ttgctttcct atttcctttt ctgattctga aaataaatct ctgcctaaaa cagtagctat
    26101 tgtaactcca aaagctgaga ccatcttaac tgatgcaaat attgcataag gtataaaatt
    26161 tccacttgct attgctccta ttgcaaatgt ataggctgct ttatttattg gtccacctaa
    26221 gtcaaatgaa accatagctc ctattattgc acctagtagt attgcattta ttccagtcat
    26281 cccatttaac caatttataa gaccattgtt aagtgctgct acaggctttc ctattacaaa
    26341 taacatcaat aaacctacta ccaaagaacc aactactgga tatacccaaa aacttacgaa
    26401 tccagctaat gtgcctttgg ctggtatatt cttctttaaa aattttacta tataaccagc
    26461 aagtaaacct cctaacatac ctccaagaaa tccactacct attaaatcag ctgctagacc
    26521 tgctgcaaat cctggtccaa gacctggttt ttcagctagt gagtaagaca tatatgctga
    26581 taatactgga accatcaatg tacctaacat ccctccagaa agctttctta attgccataa
    26641 ccaagaacct tcttgattgt atagttcttg taaaccaaat gcttgagatg ctaatactgc
    26701 aaatgcaagt atcatacctc ctgccacaat aagtggtatt atatgagata ctcctgtaag
    26761 tattgctgat tttacatctt ttttccatga tgatttttca caatatgata tatcagatga
    26821 tttttctata taatcacctt taccttgttt ttgtgctact tctaaagctt cgtatattat
    26881 ttcttttgcc ctttttagtg gagctgctac tgacgttttt atgtttggca aatgttcaaa
    26941 tctttctgta tttttaggtg ctacatctgt tgcatatatt attgcatctg ccttttttat
    27001 tatatctggt gtatgagggt ccaccatacc attagctcct tgcttttcta catacacatc
    27061 cacacctatt tctttgccag ctttaattaa tgcttctgct gacatatatg tatgtgctat
    27121 acctgcaggg catgctgtta ttgctaatac aatttttttt gattcatttt gaatcctaat
    27181 ctcttctttt atttgttctt tcttttcttt tatatctaag ttattaaata actctttctt
    27241 agtttttgaa tttaacaaca tattcatata atcttcatta gacagtctag taactaactc
    27301 agaaagtatt tgtatgtgtg tatttccacc ttcactttct ggtactgcta ttaaaaatat
    27361 tagctctact ttattatttg agtctacgct ttcccaattt tctattggtg tctctaaggt
    27421 catagctgca aatgcagctt ctttaactga cttagatttt ccatgaggaa ttgcaagccc
    27481 tctttcaaaa cctgttggag ataaagtttc tctgtacatt acagcttcta aatattcctc
    27541 ataagatgtt aacttgcctt catcatatag ctttcttgct aagaacttta ttgcgtcttc
    27601 ctttgttttt attttttgtt ttaaaaaaac aaggttttca tttgtgattt tcttaaattc
    27661 caaaattacg acctccttaa atcatttaat gttatgaata tagcataaaa taaatcatga
    27721 aaatgttttt ttgaaattat acacacaatt tcttcttaaa aaataaggat ttgtgtattt
    27781 atacacaaat ctctatctta accagtattg ttctattatt gctctcataa tcatcataat
    27841 tggaacctta tctgatatat tgtacccatt tacttgttta attgttgaat agcaatataa
    27901 acttatatct gcaatttgtt gcaattgatt ttcacatata tgtgttagag ctactacctt
    27961 acaaccctta ttaactgctg tctttgctgc ttctataact tctttcgttt ctccacttaa
    28021 actaagcgcg aaaaacatat cttgttcacc taattttcct atatcatgaa gtacttcatg
    28081 tctttggcta tagtaagtta tattatcatg cccacctcta atttctttta ctaatatctc
    28141 acataaagga atgttttgac ctattccata aactataata ctcctagatt tttgaattat
    28201 atctgctact ttatttattt tttcataatc tattagctct aatgtctttt ttatatctga
    28261 ttcaaaacca ttttttggct ctttatcctc attttttaac tcttctttta aattgttttt
    28321 tagttgagag aaaccatcat actccatttt tttacatagc ctaactatgg tatttgggac
    28381 agtaaaaaat ttatctgcta aagattgtat tgataatttt actatttctt ctttgttttt
    28441 tctcatatac tcaattatca tgtcttctgt atctgtaaat ttatactcat atttttttgc
    28501 tctttcataa aattccatag tttctcctcc atctatttta ttattcataa gtaaaattca
    28561 aaaatattag ctaaaatttt cgttaatata attataattt aatattttta atttattaat
    28621 gcatactact ctttttatta tacataaaaa atgtgtactt cctatattga tagagatgat
    28681 tcaggttttt attttgatac atctctatca atattttttt agaaaataca catttttttc
    28741 aaatacttat ttttaattaa aactttaggt aatctattct ttatatataa ttattagact
    28801 tgaaaatatc tttaaaatca accaattcaa tattatttct ttttaaccac attttaactt
    28861 ccttatcaca tagcattgca tgttcatata ctctctcttg aataagagaa gatgatttta
    28921 taagactata atctataaat cctggatgag taaccatcaa tgttatatct ttatctaaat
    28981 ttatatattt ttcttcaaat aatttaacaa aagatttatt taacttgtaa aaatcattct
    29041 gaggatattt acttggcatg ctaatacatg aatctaaata atagggacaa cttggtatac
    29101 cgtattcttt tgcagcatca cacattgctg atattgatac atcttcttct ataccatgta
    29161 tatcaatata ctcaggcaat ttactcatat acaacttgta agtttcaatt tgagcacaaa
    29221 cttcttcata tacttcttca taattaaaaa cctctttacc tgtcagtagt tgcgttcttc
    29281 ttattttgct agaaacaaat tttccacctt catctaacaa attctttatc ttaatatgtt
    29341 ctttacaaca aggttctcct attactaaat taacatgtaa accaaaacat acatcatcat
    29401 tttttaccat ttctatagct tgtcttgaaa aaggcatatt caccatgatt ccagcacttg
    29461 taacaatacc gttcttataa gaagatatta tccctagtgt aacaccttct gaatatccta
    29521 tatcatctgc cctaactact aatttcttca ttttatgcat ctgcctgctt tctcaactca
    29581 tcaagttgca tttccatctc atcctttact ttaacaacat ccgttcctat gatgatttga
    29641 actgatgtat tactcaattt aattacccct ttggctcctg tttttttaat attatcatca
    29701 ttcaaaatag tggcatcctt cacttctaaa cgtaacctcg tcatacaatt attaagagtc
    29761 actatatttt cataaccacc acaattttta attatctgct tagccatata tgaaaaatta
    29821 ccatgagaaa gttttaaatt cttttctttt tcactaactt tatcacctat ctctatctca
    29881 acttcatgtc ctggtgtttg tacattatct tttattatta tatatttaaa tgttacataa
    29941 tataatacaa agaatacaac tcctacaggt ataactaacc acgctttatc tccataacta
    30001 aaacttaaga catagtcaat tatacttgct ccattaaatg aacccaagcg agcacccagt
    30061 aaatatagta caactccacc tagaccagaa taaaaagaat gaattatata taactttgga
    30121 gatgcaaata aaaacgaaaa ttctattggt tcagttactg ttgccataaa agaagtcaat
    30181 gctccagcgg tcattgcacc ttttacttct tgcttctttt tatcttgtgc tgttttatat
    30241 atagcataag aaatagcagg tattgaaaac atcataatta tgtaaaatcc tgtccagaag
    30301 aatcctgcat ttgggtctcc tgccataaat ctaggaattt ctcctcttat aacttctcct
    30361 gttgatgttg tatattcacc catttcaaat aatatgtaag catttaaaac ttgatgtaac
    30421 ccagttggta ttagtgcacg gttacagaat aaaaacaaga atattcctat tgcaccagct
    30481 cctcccaacc aaaaaccaaa ttgattcatt gcagcttgtg gatatatcca taataaacta
    30541 aatatatatg ataaaattaa agcaaccact ggtgatagtg taattacaca tttttctcct
    30601 gcaaagaagc ttaaaaatgc tggaacttta actttatatg ttttattgta agtccatgct
    30661 atacaagctc caacaattat cccaccaaat acacctgcat ttaatgtatc attcaataac
    30721 tctaaatttg acttaaatat catatatgca actacggctg caaccattac acttgtcttg
    30781 tctttagact ttgaataagc actaactgct cctaataaaa ataagtagtc cacatttgaa
    30841 aatactatat tagatatatt agatatagct ggcacattaa gcatatttgg accaaataat
    30901 aatcctaata tacctgctgc tggcattaca gctattggca ccagcatagt ctttcctaaa
    30961 agtgacagtt gttgcatcat tttgtttaag aacctcttca tatccaatcc cctttctatt
    31021 tcggtttaat tttttgtgaa aacgatttat tcacatttta tattttaaat attatatttg
    31081 tttaaacaaa ttgtcaatat tttttattca ttttattttt atcatttagt ttcatatcta
    31141 aatcctttta agtatattgc tatatctagc ctatttttta aatttatttt ttttattttt
    31201 aaaatatgtt taaccatgta tttgaaacaa cccacaaaat agttcaaatt tatccttttt
    31261 atggtttata taattctatt ttcactatac atgtctaaac acatttgata taatttgttc
    31321 atatcagttc aataatatta ctaaagacct ttaaggaggg ataggggtat atggctaaaa
    31381 ataaattttc agagatatat gaagtattga aagaggaaat acttgatggt aaatatacaa
    31441 gtaatatgat gctgcctact gaacttcaac ttattgaaag gttttcatgt agcagaaaca
    31501 ctgtacgtag agcaatttct caattaaaca cagaaggata tgttcaaagt ataaaaggca
    31561 agggtgttgt agtgttagaa aatagttgtt ctaatgattt ttttctaaat atgcacaatt
    31621 tcaaaggtgt agaatcgatt gtagaagata aaaaggttaa tactgctact agtgttctcc
    31681 acttcagcaa aatcttaatt gataataagt tatctaaaaa gacaggtttt aaagttggta
    31741 gtgaagtata ttaccttcac agactaagat atattgataa catacctaaa attctcgaca
    31801 ttaactattt tctatgtagt atagttaaag accttgatgt ttctatagca caaggctcta
    31861 tttataaata tatagaagag tgtataggta caaaaattgt ttcatccaga aagatattta
    31921 aaattgaaaa agctacagag cttgagttaa aaacgctacc tctaaatgat tataattgtg
    31981 ttggtgttat taaaaatagt gtatatactg atgatggaaa gctctttgaa tatacagaat
    32041 caaagcatac acctgaaaca tttgtattca tggatgttac tcaaagatat taaaaaaatt
    32101 atgtttaatt aaacataatt tttttacgtt tagacacttt atcccatact aacctcacta
    32161 ttctatatac aattatatgt ttatacaaat aattatttat tgtatatttt agctttaata
    32221 ttagtttaat acttgacaat ttgtttaaac atattataat taacttataa attacaggag
    32281 gtatcaatta tgaaaaattg gtggaaaaaa gcaactgtct atcaaatata tccaaaaagt
    32341 ttcaaagata gcaacaatga tggtataggt gatataaatg gtattataga aaaattagat
    32401 tatctgtatt ctttaggtgt tgatttatta tggttgactc ctatgtacgt ttctcctcaa
    32461 agagataatg gatatgatat agaagattat tataatatag acccaaaata tgggacaatg
    32521 agtgactttg aaaaactctt aaaagaagct cataaaagag acataaaaat aatgatggat
    32581 atggtcctaa accatacatc tactgaacat aaatggttta aagaatctaa aaaaagcaaa
    32641 gacaatccat atcgtgatta ttatttctgg aaagatgcta aacctgatgg ttctgttcct
    32701 aataactgga tatccagatt tagtggaact gcttggaaat atgatgaaac tactaaccaa
    32761 tattatttac atttatttga agaaactcaa gctgatttaa atttggaaaa tgaaaaggtt
    32821 cgtgaagaat gttacaaagt actagaattt tgggctgata aaggtattga tggttttcgt
    32881 ttagatgtag tcaacctact ctccaaaaca cctggattac ctgatgaccc tataacaggg
    32941 ccaaaaggag atggaagaac tcattacgct gatggtcctc gtatacacga atatttacat
    33001 aatatgaatc aaaaagtttt caaacctaaa aatatagtta ctgtaggtga aatgtcatct
    33061 actacacctg aagagtgtat caactatact agagaaaatc gagaagaact atctatggtt
    33121 tttagcttcc atcatatgaa aacagattat aaaggaggcg caaaatggac taatgaaatg
    33181 ttctctctgg ataaattaaa aaaagcacaa tctgactttc aatataaaat gtatgaagga
    33241 aagggatgga atgctctatt ttactctaat cacgaccaac ctagagcttt atctcgattt
    33301 ggagatgata gattttttcg tcaagaaagt gctaagatgt tagccataac actatttggt
    33361 ctacaaggta ctacatacat atatcaaggt gaagaaatag gtatgacaaa tgcctacttt
    33421 actaaaatag atgaatatga tgatgctgaa agtaagaatg catactatca tatgattaaa
    33481 tctggtattg atgaaaagga ggcattgctt atacttcaac aaaaatccag agataatgcc
    33541 cgtactccta tgcaatggga taatactatt tataaggggt tttctaatca taaaccgtgg
    33601 ttaaaagtta ataatgacaa tattagtgta gaaaatgagc ttaatgattc tcgttcagta
    33661 ttttatactt atcaaagatt aatcaaatat cgtaaacaat atgatatttt tacagaaggt
    33721 acttatagat tgttagatga aaatcataaa aatctatttg tttatgaaag agagtataaa
    33781 aaccaaaatc ttcttgttat aagtaatttt actcatgata atgtagtata taaattacct
    33841 gaaacttatt tgaataaatt aaactacaca atacttgaaa ccaattacac tagagatata
    33901 attgaaaata aaatggaaat aaaaccttac gaaagtataa tgatatatta taaataaagt
    33961 tatttatata aaaattggta taacaccagg gtgttatacc aattttacta aattcaaaat
    34021 aacatttata gaatatctat agattacaat aattatttga cttctacatc attatatttc
    34081 tttgaatata ctttagtcat atagaaatat ataggaacta caattatcat cccacctaat
    34141 ccccaaacta tcttcatagg ttcatcaata cctgctttta tcagtaataa tatacttata
    34201 ataactgcaa atattggtat aattgctcct ccaggtattc taaaatttac atctttatca
    34261 gcatacttct ttctcaatat tagtacagct aaacatgtag gaatatattg aataaatcta
    34321 gatacaacac ttatttgaga taatgttgtg aatgaacccg accatgctat tagtaaagtt
    34381 cctatgacag atactataat tgctatataa ggagctccat ttttattagt ttttcttata
    34441 gacttaggca ttaatccttg ttcaaccaaa gcagcagcag accttggtgt aaatatggat
    34501 gatgcaatat ttatacctcc aacagatact atagttccta ttgtaattat atacccacct
    34561 ataggaccta gcatcttaat ggcagcatct gcaacaggag tagctgaccc tgccaagcca
    34621 ttacccaaca cccctatact tattcccagt ataagcatat atattactga tactacaaac
    34681 attaccatta ccaatgcttt aggtacattt ttcttagggt tctccatatc ttttgctgca
    34741 acagctaaag attcaaatcc tgtaaaagca taaaataaag ttatagatac tgctacaaaa
    34801 gctgatgacg cactttgacc cttttgtatt attacaaatg gggtaaaatt agaacccttt
    34861 ataaagaata atcctacagc tataaataaa actattggta ataattttcc tatagtcatg
    34921 atattattta agtgcttaga ttgtttcatt cccataaaat ttataatccc aagtcctaca
    34981 actaatatac caattattat atttttaata agtggttctg ctgcttgtgg ccatacagat
    35041 gatagtgtta cagcaaaaaa atttgccata gtagcccaag caatcatact aactatccac
    35101 ttcataaatc caacttcaaa tccaacaaaa tctccaaatg cctcttttgc ataaagataa
    35161 ggccctccat cagtatcaaa ccaactacct gcttcagcaa aacataaagc aatagaaaca
    35221 gctaaaagca tacaaaaaac taatactaat atacttgctg gacctatatc tgtataagca
    35281 tcaccaggta acccgaatat acctgaccct attattgcat tgattcctaa tagtacaata
    35341 ctccaaaaac ctaacttaat gcctttttcc attgttattc cccccaaagc caacttaata
    35401 tatttatatt ttttatttag atgcttcttt aactattgcc ataaatattt ttttcatatt
    35461 atcataatct ctagtcatca tttctggatg ccattgtatc cctattacaa actcacttcc
    35521 ttccttttct atagcttcaa ttaaaccgtc tttagatgta gctgaaacaa tatatcctgg
    35581 tgctactttg ttgacaacta agtgatgaaa actattaact aaaacttctt tttctcctaa
    35641 tatttcatat aattttgtcc cctcctttat ctgtactgta tgtgtaggta cattagatag
    35701 atgttgctga ttgtgtttaa tataagcacc ttcaattaaa gataaatctt gatacaaact
    35761 acctccttca gttacattta taatttgttc tcctctacat atacctaaaa ctggtttttt
    35821 catatctaaa gcatgtttta ataatttcat atcaaaacta tctctctttg gtgatattgc
    35881 ccccaactta ttatgaggtt cttctttcca tatgagtgga tttacatctt gtcccccaga
    35941 tagtataagc gcatctatat tagatacctg ctcctttata atctcatcat catatacaat
    36001 cggtacaata tatggtatag ctttacacat aactactgat tgtatataat cattatttac
    36061 ataagccctt tcatagccag gaaacattcc tccttcatca attagtaaat tacctgatat
    36121 tcctattatt ggatatttct ttttaaccaa tttcttcacc ccttaactta tttgtgaaca
    36181 ataaaaaatg ctacttctct taaagagcaa gtagcatacc aagattttat ttatttgaac
    36241 attataattc attttagaaa tacttttttt gaatacaagt aatataaata actttattta
    36301 cttatatttt attttaatcc tgtttagttt gactttttta aattgtttta agtacaacta
    36361 ataggtaaaa taggcattta gattttcctt tttatcaacc tattaaatac tgtttaatat
    36421 attttttccc cttctattta attcacttcc ctttctacca gattcacata ttatcaaatc
    36481 cagctctttt aacatattaa gtattcccct tacctcacct tctgttatac tgtagcctaa
    36541 ttcatttatt gaattaacaa taccatttct acctatattt ttattaagat tattggcatt
    36601 ttttaaacac tccattacta actttacttt ctctatagat gttctatcct ttaatatgct
    36661 tatttcttta ctaaaatctt cagttatatt taataagtta tgaggtaaat catttattaa
    36721 aataacatcc tcagaactca taatgttaat atacatagca gtatttttta attctcttat
    36781 attacctggc cattcatatg cattaataat gtcttgaaca ccttgactaa ttgctctctt
    36841 ccttttcata aaatgcttta acataacttc tatatcttct tttcgctctc ttaaaggtgg
    36901 tatatttatg ggcaatacat ttatcctata gtataaatct gctctaaatc tatctccttt
    36961 tatcatttct aataaatttt tatttgtagc cgcaattact ctaacatcta tatctattac
    37021 attttctcct ccaaccctca ttacttgatt ctcttgtaat actctaagta atttgctttg
    37081 taagtatata ggcatgtctc ctatttcatc caaaaatata gttccattgt tagcctgttc
    37141 aaataatcct tttttacctc cttttaaagc tcctgtaaaa gcaccttctt catatccaaa
    37201 aagttcactt tcaagtaaat tttctggcat agctgcacag tttatcgcta taaatgactg
    37261 actatgtctt ggtgatgcat tatgaataga ctgagccata agttcttttc ctgttccact
    37321 ttctcccgtt ataagaattg tataatcaga ttcagctatt ttcataccta atttcttagt
    37381 tctttccatc ttcgttgaaa ctgtttttat atcatcaaaa gtatatctag caacttgtcc
    37441 cttttctcta agcttctttg ataggttttg ctccaatttt cttatataag ttatttcttg
    37501 aatacaaaaa taatacccag taacccttcc aaaactctct atattatact tatttacgtt
    37561 taagtactta ttattaaatt ttataacttc atcatgtgcc ttactcaaac taaaaatctt
    37621 tttaatattc tcattctcta attcatctat atttttccct ataatattac tttgaatatc
    37681 tagtatatcc cttaaactat tattgcaaat aattatgttt cccttatctg ataccattgc
    37741 catacctaag tttgataaat ttattataga atttaattca tctgctttta tagtaagttc
    37801 tttgtattta gtatttattc ctgaatataa actaattatt tcatctgtat acttaattag
    37861 cctctcagta atttctttat catccagttt gagttttgct attatttgaa taaacgttga
    37921 actatcaata tacctatggc ctaaatcaat aacttcttct atatattttg gaactaaact
    37981 tgactctcct ggcgttatag ctatcttaat atttctatat tcttcattgg gattgtatgg
    38041 tataaaattc aaatggttta ttcctatatt gtataaatta gatattgttt ccaatacagt
    38101 atgcttatta tcgtttacta ctaatacatc tatttcttct ggcaatgaaa ataacttata
    38161 tatatccttt tgctttatgc tcctatttat agttattatc tttgagacat cttctacata
    38221 tttttttatt tttaatactc tatcatctat cattaccaat attatgtcat cttttattaa
    38281 ttccttctct gttattttat ttaaataata attttttatc attactttat ctttaaatat
    38341 catttctaga tttttattta agaaacattc taatttagac aacccatcag ttactactgc
    38401 tattgttttc atttgattac tccttttaaa atatactcta ctcttttata atatacatct
    38461 gattttcact ttaatagcac aaaaaattgt acatttagta tactaacata ttcctttatt
    38521 agttttatgt ccctaaatat catagttgca atatctatca tatttcttaa acttctataa
    38581 agcaaaaatt gtgccaaatt aataattttc ttaattttat tctaaagttc ctcaatagta
    38641 tttacagact tagcacttat agcttataag tctttatatt attaatctat ttttataggt
    38701 taattgtaca aatcatatat cacttttacc tattaaatgc attataaaaa agatggaata
    38761 acgattcgtc actccacctt ttttgtaata aaaaattgta tatataaaca ctatttacta
    38821 ctcttctaag tacaaacagt atctacatac tataaccttt caccattaga ttctattact
    38881 tgtttatacc agtcaaaact ctttttctta atcctagaca agtctccact tccatcatca
    38941 tacttatcca cgtatataaa cccatatctc ttggtcatct caccagtaga tgcacttact
    39001 aaatctatac atccccatgg agtatatcca aataaatcaa caccatcttc tacagcttcc
    39061 ttcatttgct ctatatgtgc ttttaaatac tctatacgat agtcatcatt tatcactcca
    39121 ttagattctt ttttatcata agctcctaaa ccattttcaa ctaccatcat aggtattcta
    39181 tatctatcat aaatttcatt tagtgcatat ctaagccctt cagggtctat ttgccatccc
    39241 cattcactag acttaagata tggatttttc actccaccta atatgttgcc acttgtatta
    39301 tcttctttag aatcactact tacacatgta gacatataat aactaaatgt ataaaaatct
    39361 acacaacctt cttttaaaat ttctaaatct cctttttcca tttttatttc aatattattt
    39421 tctccaaaat atctattcat ataagttgga tattcgccac gcacttgtac atctccacag
    39481 aaataattca ttactctact atctctttga gttttaactg catcatctgg attacaagta
    39541 taaggataca ttgttgcaaa tatttgcata catccaactt gataatttga atctatttca
    39601 tgagctaatt taacagcctt agcacttgct acaaattgat gatgtaaccc ttgaaaacga
    39661 agttgaggta tatctacttg gtttataaaa tctgtagtcc cttcatttaa tattccttgt
    39721 gataagtagc ctcccatagg cattgtagct gaatttattt cattaaatgt cagccaatat
    39781 tttacttttc ctttatatct tttaaatatt gtttcacagt aattcatata caagtctatt
    39841 acctctcttg aaacccatcc attgtatttc tctgttattg caaatggtat ttcatagtga
    39901 gatattgtta ttaatggttc tatattatat tttaaacatt catctatcac attttcataa
    39961 aatttaagtc ctgcttcatt tggttctttc tccattccag ttggaaatat tctagtccat
    40021 gctattgaaa atctaaatac cttaaatccc atttctgcaa ataaagctat atcttctttg
    40081 tatctgtgat aaaaatctat tgcttcatga cttggataga atgttccttt ttctataact
    40141 ctcgttattc ttttacttac tttttgagaa cccccagtac acatatctga acaagaagga
    40201 cctttcccat ctaaattcca tgctccttca cattgatttg ctgctgttgc tccaccccat
    40261 aaaaaatctt ttctaagtac cattactcta cctccaataa tttatttata gtaaatttat
    40321 tttaaaataa aaatacatat atctaatttt taattaataa aactctcttt taagctaaaa
    40381 cagttatcaa ttcctgacta ttatttacat gcttttgtgt attttctata atatccacat
    40441 aatcagaata attagtaaca actactggtg tttctaagca atatccttct tttgatatgc
    40501 tttctatatc aaaagttatt aatgtctgac cttttttaat cttatcccct tgattaacaa
    40561 aagatttgaa atgtttacct tccaactgaa tagtatttaa ccctatatgt atcaatagtt
    40621 cacaaccatt atcagatata attcctattg catgttttgt tggaaataaa gtcattatag
    40681 tcccatcaaa tggtgccact acttttcctt ctttaggtgt tattgctaca ccttttccca
    40741 atatacctgt tgaaaacgct gcatcttgta tctgctccaa agcctttact tcacctttta
    40801 ttgggctata tactacttct cttttgtttt tttctacaac gccttgccca ttattatctt
    40861 tagaaatttc tactatagaa tcatctttcc aaaaaaataa tgttaaaaca aatcctacta
    40921 caatagctac tattattgct attatagttg cataaacacc tttcatatct ccagttgttg
    40981 gatttatata attaggtata ccaaatattc caagtccacc cattacataa ctttttacat
    41041 tcattatccc cattacagct cctgccacag caccacctac acaactatat atgaatggtg
    41101 tttttcttgg taaagttatt ccatatattg caggctctat tacaccacat atccctgaaa
    41161 taactgcagg tatacacagc tcttttatct tcttatcttt taatttaaag tacattgctg
    41221 ctactactga tgtttgtgca aaagatggta caaatgctcc cactaatatt gtgtcaaacc
    41281 ccatagtcat aaggtttgac atagctattg gtactaatcc ccaatgtaga ccaaatatta
    41341 caagcacttg ccacaataaa ccagttaata caccacataa aattggtgaa aagttaaata
    41401 aagctaaaaa tccatttcca actatattag aagcataggt agctataggc cctataacca
    41461 taaatcccaa tggtaaagaa actaataaaa ctgccattgg tacaaagaaa ttctttacta
    41521 catctggtat tattttagca aacactttct ccaacttagc tgctaataat actattataa
    41581 ttactggtac tactgttgaa gtgtagttca ttgctattac tggtaatcct aagaaagtta
    41641 tatatactgg tgattcaaat attgttccag taaatagatt gtataaaggt tgacctatag
    41701 atgctagagt atctaattga atacctgaat agcataaaac tgcacctata agcattccaa
    41761 taaatggttt taatttgaat ttcttagcag ctgtatatcc aagaattatt ggcatatact
    41821 ggaacattgc atctccgatt gcatttaaca taatatatgc accatcagtt tctccataca
    41881 aacccaatgc tgtaaatact gcattaaatc cttttatcat accagcagca cacagaactc
    41941 ctaatattgg ttgaaaaact cctgatatta tgtctataaa cttattaagt aatccttttg
    42001 gcgcatctga atctgaatta tctattgtat ctcctcctaa tccagccaaa tcgcacacaa
    42061 cttcataaac atctggaaca tgattaccaa taactacttg gtattgacct gcacttttca
    42121 ttacagtaac tataccatcc atttttttta gcacatcatc atttgctatt gattcatctt
    42181 ttaatttaaa acgtagcctc gttatacagt gagataaact gctaatatta tccttccctc
    42241 ctacattctc aatgatttct tttgatagtt gttcatatct tctcattttt tctcctccat
    42301 tttctttttg aaatatgaag gtttggccaa cttaatgtca ccacccaata tatataaacc
    42361 taattattag gatttatatc tgacttaata cttttactta ccactctttc aacgtgaata
    42421 gttaagtata atttttcctc atctgttaaa tcgtagttgt attttttatg tataaattca
    42481 tccaactttt ctatgcattt ataagcattt ttatatttaa cttttattac atttagtaaa
    42541 tcatcttcat tattttcatg taaattatta ctaacaattc gttctgcaaa atactttaag
    42601 tgagtaataa atctatgata ataaactgaa tcttcatcaa atactattct gtaatggtat
    42661 ttaaccatat ttgaaatttc ttgtattatt tgtgtaatct catatatagt ttttatactt
    42721 tgatccattt gtgcattaac tatatgcagt gctataaatc cagcttcatc ttctgatagt
    42781 ctaatttcaa attttttttc tatataatcc aatgcttcta accctatttt aaattcactc
    42841 ttataaaatc tttttatatc ccaaagtaat acatttttta cacttactcc ttcacgagct
    42901 ctttctattg cagtatacat atggtctgtt aaagaaatat aaatactatc atttaatctt
    42961 ttccctagtt tttctttagc atataaaatt atttcatctg ataattccat atatcttata
    43021 ggaatatctg ctattagttc tttaaattta tcagatatat ttgggtctga aagtttgaat
    43081 atcttatcaa ttttatcctt tgatatatgc tctcctgtac gttttttata agctaaacct
    43141 ctccccataa ctactatttc cttatgattt tcatcagttg taataattac attattattg
    43201 agtatttttt caataatcat tgtgatgccc cctttattag cattaagtta aaataaatct
    43261 tttttaatta tttagaatat ttaaaatgta atttattaca taaaaaaaaa cctatttatt
    43321 acacattaaa tagactccca tgagtcctga gtaataaata ggtttagctt gtatttaaac
    43381 aatcactatc cgtttgtatt ttattttaat tattatttat tatatgttag caaatttatt
    43441 ttttagtgtc aactttttct caatatttta attctaaata aattatataa attgcatctg
    43501 caaagtacta tttttataaa taaacaaaat aaaaaaggta ttacttgctt taagtaatac
    43561 ccatttattt ttaatttaag tggtttttct taatctcatc aagtagtcca tcttctaaat
    43621 aaatttctaa tgcacatcct accattgcct ttactgcttt gtgaagttta tcataagctt
    43681 cctcagaatt tgcataattt agaaattctt tttcatgaac atataccatt ggacttatat
    43741 ctaaggctat ttctgtatac atagtagggc aaacttgact tacattacct atatctgtag
    43801 aacctactgg accatcttta ccttctaata tatctgttat acctgactca atcaaattat
    43861 tctttgttat cttttgaagt actttgttat ttattatgtt gtcaaaagaa ttttcaaagt
    43921 atctataaga aagcttagct cctgtcatta actctgcacc ttttgcacag tttataactt
    43981 ttttagtcac ttcatctaaa taacttcttt cagcagctct tacaaaaaac ttacaagctg
    44041 ctttttctgg aactatattt ggagcctctc ctccatttga aactattcca tgtattctaa
    44101 catctgattt tacatgttgt cttagtgcat ttatcccagc aaaagtaagt tgtactgcat
    44161 ctagtgcatt aactcccata tgagggtgac tagctgcatg tgctgatacc ccttcaaaac
    44221 taaactctaa tgaatctatt gccaaagcct tacagtttat attattaaaa gcttctatat
    44281 gcatttgata acttacatca acatcatcaa aagctcctgc cttaactaaa tcacattttc
    44341 ctcctgttgt ttcttcagct ggtgttccta ttagtacaat ttttccttta aaattttctt
    44401 ttaacttact taatactata caagctccta gtgtagatgc tgcaatccag ttatgcccac
    44461 aagcatgagc tggttcttta ttgtctccat atccaggaag tgcatcatac tcagctaaaa
    44521 atgctatggt aggtccatca ttatctccta attcagctct aaaagcagtc tccatattac
    44581 aatatggata aactgtatca aatccatatt ccttcatttt ctctactaag tattttgatg
    44641 atatgtattc ttcttcccct aattctggat tgttaaatat aaaattacaa acttcttcaa
    44701 tttcttttcc aagttcattc tctatctcat aagctctctt ctttatactc tctatcataa
    44761 cttacctccc actagaatcc tagatataat cctattgtca aaaataccat tacaagaatt
    44821 gttaatacta ccattaaagg aattacgaat ctaacccact ttgaccaagg aatattggct
    44881 actgctagtg ctcccattaa aacacctgat gtaggtgtta ttaagttagt tacacctata
    44941 ccaaattgat aagctgtaac tatcatttgt cttgatacat ctactaagtc gccaagtggt
    45001 gcaagtacag gtattgtaag tgatgcaagc cctgatgctg atggtattaa aaatgctatt
    45061 aatccttcta ttaagaaagt taaatttata aatatagttt ttggtaatcc atttaataaa
    45121 tttgctgcag aatttaaaat tgtatctatt ataaatccat tttctgctat tattacgatt
    45181 ccacgtgcaa atgctataca taaccctgct gtaattaaat ctcctgctcc acttataaat
    45241 gaattaacta tctcatcttg tttaagccct gatgctattc ctgatattat acctatagct
    45301 gtaaatatca ttgcaatttc agttatgtac cactctagtc ttaaaactcc atatatcata
    45361 atcgccatac ctattgtgaa aatggctatt accatggctt ctctttttgt aaattccttt
    45421 atatttgtat cactattaac cattgattct tgatttagtg atatatcgtg tactaaagat
    45481 tttttagggt cttttttaac tttattggca tacatcataa caaatcctat agatatagcc
    45541 atatatatta taaacataac aactctaaac tctattccag aacctggtac tatttgtgat
    45601 aatgcttgtg ctattcctac agagaaagga ttcgtagtag ctgctccaaa tccagctgtt
    45661 gctcctataa atactgtggc cactgctgtc aatgaatcat atcccatact ggtcataagt
    45721 ggaataaata tcatataaaa aggtagtgtc tcttcactca ttccaaacgt cgtcccacct
    45781 agtccaaata gaatcattga aatcggtatt attaaaaatt gaaacttctt cattttattt
    45841 acagtatgag ctataacagc ttctatagcc cctgttttat taactatccc aaaagctcct
    45901 ccaactatta aaacaaatcc aactacttca gctgcatcta taaatccgtt tataggtgct
    45961 gtaaatacat cagtaattcc ttgtggattt gatggtgctt tttcataagt tccagctaca
    46021 acaactgacc ttccgtctat atctactctt tcaaaatttc cacttggcac tatccaagta
    46081 agaattgcca ttaacactat tagcgaaaat attattgtaa gtgtctgtgg catagtaaac
    46141 tttttctttt ttaaactgct catttttaat ctccccactt tttaaattaa atattttatt
    46201 attgcatccc tttatcctat tttgtataga tgtaaaaaag ctcaacatgg atacatgttg
    46261 agctaaatgt gctaaataca acatacctgc atcttccctt tatcttgttt aagatagctc
    46321 tccatcaaaa ttcgggataa ttttgatgac agtactgtac ctattaaata cagtcccagc
    46381 actagctttg gacactagca cttcggcact tactcctttt atattgcctc attggtttcc
    46441 aacctcttca atattaatct tagtaaatgc aacctctacc tcaatctgtt cgttagatga
    46501 ggtaattctt attaattttt attgattcaa agtaattgta gcataatatg tcgagattgt
    46561 aaatattttc agtataataa ttcttacttt atgatgtcat tccatttttc acaaataatt
    46621 ttttctccat ataatttaaa tactgtagta tattttaaat acacacaaac tttccacaaa
    46681 tttttaatta ttataatacc tttataataa aaaataaaag ccatgtaaac ttatcaattt
    46741 acacagcttt atatacatta atctactcca agtacagctt taagctttga tgctattgta
    46801 ataactcgtg gaccaaatat aacttgaact gagtttttct ttttaacaac tccacttgca
    46861 cccaattcat ttacccagaa accatcttca tagaatccat caattgaatt tagctctacc
    46921 ctaagtctag ttgcacagtt tgtaacattt tttatattat tacttcctcc taaaccttct
    46981 attatagaat gtacaagctt tgtatcttta tcatctactt cctcttctac tgcattgttg
    47041 tatgctgttt taccagcatc tacagattgt ctttctctat attctttctt actataaagc
    47101 ttaacttctt ctgaacttct tcctggtgtt tttatatcaa acttcttaat tgcccatgta
    47161 aatattatat aagtaactat aaaaaatatt ggtatcaaaa ataataatgg taacgcatcc
    47221 actttttgtg gttgtaataa attaggaaac atatttctaa atgattctcc cattatagaa
    47281 acattaaaca tttctgtaaa tacatacgtc aatcctgcca gtggtacata tgctacaaaa
    47341 tacaataatg gtgcaacaaa taggaatgta tactctattg gttccgttac ccctaccaac
    47401 atggctgtta ctattgctgg tatcaatata cttattgctt ttttcttatt agcattatca
    47461 gccaacttat acatagctaa agctgctgct ggtaatattg caaaatgtat taataaacga
    47521 cctgttgtga agttacgagt tatataagat agtgtatctg ggtctcccat ttgagctaat
    47581 cttatattat taacaccctc gtaaattgct ccacctactt ccattgttcc acctactctt
    47641 gtatattcta tagggaatgc aattaagtga tgtataccaa atggtaataa tgctttatcc
    47701 attgctccaa aagtaaatgt tcctaataat ccactagaac ctatccaatt accaagttta
    47761 gataatgcca accctactgg tggccatata aagtacattg ctgtacccaa tactattgat
    47821 gctataaaca tcataatcat tgaaaatctt ggacctgaga aaaaagataa tgcactagga
    47881 agttctatgt caacaaattt gttatgtact gcactagcta caaatccagc tattatagct
    47941 ccaaatatac tacaatcata tgaaaagatt cctaagaaag aaccatataa agaacttcga
    48001 ctatatgctt ctgcctctcc taatccagat gccataaacg cttctgctgt aactgtatct
    48061 gaatttatac catttgatgc taacaatgta cttataactg tgtgcattcc tataaataaa
    48121 acaattcctc ctaatgcacc ccatcctttt tcctttttag ataatccaaa agctatacct
    48181 gtaacaaaga atattggtaa gtatctcatt accataaatc ctaaatcgtt tattacttta
    48241 aagaatacat ttataaatga cccttctttt attagtccgc taagccctaa tgcagaaacg
    48301 ttttccatat ttgcaaaagc tgaccctaat cctataaata ctcctgctac aacaagtaat
    48361 ataacaggta tcatcattgc tccagaaagt acttgtaatt tatccttcat agtattattt
    48421 cctccatttt tattgaaatg ccaggctata taatgcatat atatatactt ctattgcttt
    48481 gtataatgta tctatactta tattttcatt tgcctgatgt gaatttggat tatctccctc
    48541 tattgtgggt ccaaatgcaa ctccattttt taacactctt gcataagatg cagctccttt
    48601 tgtaaaaagt tcggcttctt ttccaaaacc ttgtttatat gcacttgata atttatttat
    48661 taattcacta tctggtttta catacagtaa ttttaaatcc ttatatatat caactattaa
    48721 attttctttt ttcccaattt cattaattct attagttata aactcccaac ttataccctt
    48781 aggatatcta aagtctattt tcatattcaa ttcatttttc tctaagaaga tactcattat
    48841 acatattgta gaaactccca tatctacatc ttctttatat atccctaatt tttttccatg
    48901 attatcatct gtaaagtatg aatttaaaat atcccttatt ataacgcctt tatcatttag
    48961 ctttttaatt ttctctaaat cttttgctaa actaaatgca cagttatatg acctgtgtgg
    49021 atttctcgat aaagcccttt ctcctgtata tcttactaat actttacctt cttctagttc
    49081 ttctatctct gtataataag ctaatgattc tactaaatct ttcttatcat cagttttaaa
    49141 aacagcctca attttgtcac atacaaatcc atcttctttg tttgacttta tatcaactaa
    49201 attgtgattt ctaaatttat ctttatctat tttctttctt agattgaagt ataaaatacc
    49261 attttctcca tttactattg ggaagtttcc atctggagaa aatgcaaatt ttggctgttc
    49321 attgacacta aagtaatgct ccatacattc ccaagtcgtc tcttcagctc cacccactat
    49381 caatctaatc tttctcttcg gcttttcatt aagttctctt aagaaaagta atgcatataa
    49441 aatccctatc aaaggtcctt tgttatcatt aactcctcta ccataaagat agttatcttt
    49501 ttgacatact ttaaaaggtt tactcttcca aagttcttta ttatatattg gcaccacatc
    49561 tatatgatta agcaccccta ctacttcttc tccttctcca tattctatat gaattgcata
    49621 tccatcaaag tcttttacaa caaaatcttt gtcttttgct atttgaataa gcttatcgaa
    49681 ggcatttctt atttctatcc caaatggttg atttatatct gttgtacttt catctctaat
    49741 tgaaggtatt tcaactaaac ttctaatatc ctttatgata ttttcttgat tggattttaa
    49801 ataatcaata attagttctt tgtttactat catcttaatt atttatcacc cctttatatt
    49861 aattttacaa agcatttcag ttcatatcta cattataaat tttgaatatt attttttcaa
    49921 tatttcaaat acttctctaa attaccataa tgatacggta aacaatcaac agattatgac
    49981 aaaatcatac tatacctatt actaatattt taacttatct attacgtctt ttttaatgta
    50041 attttattcc aaaaatagat ataaaaagat gtagccttag ctacatcttt ttatatctat
    50101 tcaacttcca ttattatgct attaatatct acatttccag gttctatcct atcttttact
    50161 gttctcttaa cagaatcagc atcatccatt ataactacag gagttattat atttaaatca
    50221 tttgacgtta taaaatctaa atctacttcc attattaaat ctttagcttt taccttatct
    50281 cctgccttta ccttaacatc aaacggcttt ccttctagtg acattgtctc taaacctaca
    50341 tgtattaata tatttgtatt atcattactc ttaatcatta ttgcatgctt tgtatcaaat
    50401 acattgacta tttcaccatc aacaggagaa tataacttac cttcatttgg tattattgct
    50461 actccatctc ccatcatctt ttgggcaaaa actgcatctg gaacatcttc taagttcact
    50521 aaatttccct taaaagggct atatattttc tcaatcttat tttctttttt cttaaatata
    50581 ttaaacataa cacactctcc atattataaa attttatgat aatatttttc ttaaagcttt
    50641 tgctacacca tcatttacat tagtatcagt tacaaaatct gccatagatt ttacttcatc
    50701 tgtagcattt cccatagcaa taccaagacc tgcaaattta atcatggata tatcattttc
    50761 attatcacct atacaaataa cttctttgct atctatacct aatatatcac aaaatttttt
    50821 aactgcaata ccttttgaag tacctttact cataacttca aaatttatct ttccagaact
    50881 tactacttct aaatcttctt gttttttaaa ttcttccttt gcttctctca aagaatctac
    50941 gttttctgag aaaagtatac attttaaaat ttcattatca gctttttcta ataaagtttc
    51001 acaacttgca ttttctatta tttcaattct gtcttcttct ttatttttac tatttattaa
    51061 tctatatgga tgttcttcac ctatttcatt gttggatata actattttac agcctttaaa
    51121 atgtttgttt aaattatact tttcacctat tgtatagatt ttctttatag cttctttgct
    51181 caaaaccttc ttatactcat atcccattaa tttaaaatat gtcccattag ttgatataat
    51241 atatatatca tcccctaaaa cacttgtaaa atatttcata gcattataag gtcttcccgt
    51301 aactaaggct accttgatac ccttttccat agcttctttt agtgctttaa tattttcttc
    51361 tgatacttca aatcctttcc ccatcaaagt accatccata tcagtacaaa ttaatttata
    51421 tcgcatttgt atcacatcct agtaagattt ttctaatatt tcaacaaggt cttctttctt
    51481 tccaaaaggt aaaaatccta aaactggaca gcttgttatt tgttctaatt gttcctttgt
    51541 tacattaaac tcagacattt ttgtcttaag tcccactctc tttaaaaaac tttctatttc
    51601 atttcctaaa gttttagctg cttcatcatc ttccactttt tctaattctt ggtttagcat
    51661 tctcgctact ttggcaaatt tctctatatt atccttatag aacttttctg caaattttgg
    51721 gaacactata gccaaagcct caccatgagg aatatgtgta actcctccta ttatttcact
    51781 tagtggatgt ggtgctgctg ctccagcatt tgccagtgaa cttcctccta atgtatctgc
    51841 tactgctaac tttgttctgt attctatatt atttttatct tccattactt taggtagata
    51901 gttaatgact aattccatag atttttcaga cattatctct gtaaagtcat tagcatttgg
    51961 atttatataa ctttcaaatg catgagtaaa agcatcaaat gctgttgatg cagtaattct
    52021 ctctggcaga gttaacataa gctctgggtc aactatacat tcttttgaga aacaattttg
    52081 atgaaatatt gtatttttct cgcttcctct cgatattact gctgcttgtg taacttggct
    52141 tcctgttcca gaagtagttg atacagctat taatggtaat tctttttcac ttaccttatc
    52201 gtatatttta aatggatttg tatatgtact aaacatataa tcccaatcta ttttttctaa
    52261 accaaaagtc aatgctaata cttttgcagt atctatactt gaacctcctc caacagctaa
    52321 aacaacatct acagattctt ttttagctaa ttcgaaccct tcatttatta attctacagt
    52381 tgggtttggc tctactctat caaagtgtac aacttctata cctttttcta ttaaattcaa
    52441 tgtaactctt ttatataact tgtctaatgg ctctacattt ggagtagtaa ctaacaaaca
    52501 tttctttcca tattttccta ttattctagg tagttcactt aaagttcctt gtccaaaatt
    52561 tattcttgat ggttgataaa agttaatcat atttttcctc ccttaaatta ttgacttcat
    52621 attcaaatta tacatatcat aggattttta aggcaatacc ttcactgtta actcttattt
    52681 actaaagctt atggcaatta ttagacaata aatgttttat tattaccata tcattatgta
    52741 aattttatta agtacccatg ttataatttt ataatcaatc ttattgtata attgacttaa
    52801 taataaaaat agagtatata cactttttta tgacaaatag cttaaaggag aattaaatta
    52861 tgaaatattt gatagacagt gctaatcttg atgaaattca aaagtgttta aagtatggat
    52921 ttaagggaat aacagctaat ccatctatgt atcttaaaga gaaggtaaac ttccatgaat
    52981 ttattgagac atgtactaat ttaaatccag aaatattaac tgctgaagtc attggaaata
    53041 atttatacga attaattaat tcatgtgata agatattaaa tataaataaa aacactataa
    53101 taaaaataaa tttctctgaa gttggcttag aacttataaa catattaaat agtaaaggtg
    53161 taaaaactgc atgtaccttg attttcaatg taaatcaagc atcattagct ataaatgcag
    53221 gtgctgatta cctattccct tttataggta gaaatgatga aaatgggaat gatggtataa
    53281 aaaaccttac agaaatatgt aaccttatat caataaatgg atataatact aaagttgttg
    53341 cagcttctat taaaaatgta ttccacttaa ctcaagcatc tctttgtgga tgtgattatg
    53401 cagctgtaac atatgattta tttaaaaaag cttcttttaa ccagcttaca gtagatggtg
    53461 ccaatacttt tgaaagagat tggtccaagg ttaataaata gtaactaaga tttaggaggg
    53521 tttgcattga acaaaagatt attagatatt gctgaattat tactcaacag ctcagactat
    53581 attactgtag atactattgc cgaagaatta aaagttagta ataaaactat tcgtaatgac
    53641 cttgttattt tagatgaatg gcttttagag tttaatttat ccctagataa aaaaactggt
    53701 tcaggggtta ttattctagg taatgaagat ataaaactta aagttattag agatatcaat
    53761 gataaatcaa attgtatata tgcatactct cctgaagata gaaaaaggta tattctaagt
    53821 aaattattta ttaaaaattc taagtttagg attcgtgata tttgcaatga actacatgtt
    53881 agtagagcta ctgttcataa agacttggta gttattcaaa attttttgca aaactttaaa
    53941 gtgactctag ttagaaaaac caataatgga gtatatttag agggaaaaga gaaagatatt
    54001 cgaaaagcag tttttgaact tgctactgtt aataaaagtt atactgaact taaagaaata
    54061 ttattctcga actctaatga atgcaaagat accccatcaa caaaaatatt taaggagcta
    54121 tttaactatg attttgagaa attatctcaa atcactctac acaccttaca agtagaaaaa
    54181 agtaacctta gtgatgagta ttatataaac ttcttaatac atatagctat atgtattaag
    54241 agaatttcaa ttaaaagata tattaactta tctgaaggtt tttttagaga attattagaa
    54301 catgaatact ttgaaaaatc aaaaatttta tgtgctaacc taagtgaagc ttttgacgta
    54361 gaatttattg atgaagaggt ctgttatata cttcttcata tagaaggatt acttaaatca
    54421 ataaagaaaa ctactactct tgaacctctt aaaaatgaag aaattacaga gtataatcta
    54481 tctgaagcaa tagctgttca ttggggaagt atactaaatt taaacttaaa ggatgattta
    54541 atattaataa agtctcttgc taatcacctt aaatcagttc tacacagaat aaactatgga
    54601 tttactatta caaatcccat attaaatgat ataaagagaa catttcctta tacttataag
    54661 gctgctaaag agtctaatac tgttatcaaa cagttgatga attatgaagt caatgatgac
    54721 gaaataggat atcttgcttt atatttaata tcagcaatag atagaagcaa atctccccta
    54781 aatactattt taatatgtca ctgtagtgat ggtgtatcta atttattagt ccaaaagtta
    54841 agttttgaat ttaatcagat taatatagtt aaatctatac ctctatcttc tatttcattt
    54901 acgaactttg atgatgtgga cttaatacta accactgctc ctgtagactt tgaacataat
    54961 gctgaactga taaatataaa tgctctttta agtaagaatg atatttccag acttagtaca
    55021 attataaaaa aactatattc tgaaaaaaat aaaatattat cttataaata aacaaaaata
    55081 ggcttgtctc aacatagaga taaacctatt tttcattgtc caaatgataa aaatatattt
    55141 gatttcatag aaaaagtgcc tcatgaacta aaaaattcat tttgagacac cttcattttt
    55201 tatatttaat ctttttattt aagcttttat attttttaaa cttttatatt ttttaaactt
    55261 ttatatttaa agtaaatttt attattttta ttgagcgcaa gcattattta tattattaat
    55321 ttcttcagta ttcttaccaa actttgtagt tacaaatcct attgttttta ttatgagtaa
    55381 taaggatatc aatgaatata tagatatcat aaaattctta cttattattc cagatgctac
    55441 tattataaat ccatctagca taaataatat aactggcaac ttcagaaact taattacctt
    55501 attaataagc attgcaacta aatcagttcc accagtacta cagcccctta ttattgctat
    55561 ccctatagca gttcctacca ataacccacc tatcactcct gctactaata gattgtcagt
    55621 tatgtgagtt tgtggtatta cttttaagaa taatggaccc ataagagtta tatataatgt
    55681 ttttattcca aaacctttcc ctaaaaataa taatcccatt actaaaagtg gcatagatat
    55741 tcctaatgat atattctcaa caggcattga taagaatgtg ctaagtacga ctgataatcc
    55801 tgttatccct cctggagcta atgtatgttc tgcaaagaaa aaattaactg ctaatgccat
    55861 taaaaatgct gatattgtta tcagtaaata atcttttatc atttcttttt tcatttaata
    55921 acctcctatg attatattgt ttcagtataa aaaatcaatt aaattagtct agcttcctgt
    55981 agtttttcat aaaattaatg cttccttaaa acattaagaa agcatctctt ttatcatagt
    56041 ttattatctt attctaaacc aaactttgta gctgcttcat ctatagcttt tctacaattc
    56101 ttaacacctg ctgttatacc atctttatcc ttgaatattg ctgaagataa tattacaacg
    56161 tctggattac attctattaa tggctccata ttgtctgctc ttagtccacc atctattgct
    56221 aattcacact taggattctt ttcgtctatc atttttctag ctctttttac taaatcaaca
    56281 gcactctttc tccatcccca gttatctttt ccatcagttt catcaacacc atgaacgact
    56341 atttgcaatc tatctatatc atatatagct tcatcaacaa aacatagtgg tgtgtaacat
    56401 ccaattgtta atcctacttt cataccaaat tctctacagt aatttattat gtatgcaaga
    56461 ggagcgccta aaaaatgttc tgctggtatt atcaacaagt tacatcctgc tgctgctatt
    56521 ttttctataa ataatctatc acaatctctt gtgtatatat ggcattctat tggtttatct
    56581 gtcactggtc ttattgcttc tattatttga tgaccaccca taagttgcat attttttaag
    56641 tcatgcatat ctgctgcatc tgaatgtacg tagtctacac ctgcatccat tgcttcttta
    56701 actacggctg atatatgacc gtaatttaca tgtgctaatc ctgctgctat ttttatgtgt
    56761 ttcatatttg tatacctcca tattttactt taattagtat taaatattag tttttgatat
    56821 tttttaatcc agtattactt ttatttagca ttacttttat tgctgtatat atttctttta
    56881 tgaacttatt ctgcttttgt taactgtgaa cttattttgc tttgtaaatt aatattacac
    56941 tgatatattt tttttcacaa cactttgact actccttaaa agtaccttta tattgtggta
    57001 aattatagac attaattgta tataaaaagt tagtttttta ccacattctt atggcattta
    57061 ttgtctatac taactcaatt gatatagcta gttcatttaa tatataatca gactataaat
    57121 ataaatttac tcgataaaaa gtaccattca taacaatatt aatgaaaggg agatgtaaaa
    57181 tgagaaactg tattattgcc caatctggag gccctacagc tgttatcaat gcttctgcag
    57241 ttggtattta tgaaaaaaat ttagaaacta aatactttga taaagtatat tttggtatca
    57301 atgggattga aggtatatta aacaaaaata taatagattc agactcctta aacaaagata
    57361 atgtattaaa tttaaagcaa acaccttcgt ctggtcttgg ttcttgtaga tacaaactgg
    57421 ctgattataa aataaattct tatgattatg atagagttat aaatatacta gatgaatatg
    57481 aaatagatac attattttat ataggcggaa atgattctat ggacacagtg atgaaatttt
    57541 ctcaatactg caaaaacact acttgtaaaa taaaatttat agggattcct aaaacaatag
    57601 ataatgattt attaggaact gacaactgtc ctggatttgg ttctgctgca aagcttattt
    57661 ctacactaat aatggaaaca tacttagatt tttctatata caaaaataat ggtatattta
    57721 tagtagaaac catgggtaga gatactggat ggttagcagc ttcttcttca ctagctagac
    57781 ttaatgatac tccacttgtt gatttaatat accttccaga aattgatttc tatgaagata
    57841 agtttataga agatgttaga aaaatattta aagaaaaaaa taaggcttat atagttgcat
    57901 cagaaggtat aagggataaa tacgggaact ttatatgcga acaaaaaact tctgctcatg
    57961 ataaatttgg tcattcccaa ttaggtggag taagcaacta tctaaagaat cttatagtat
    58021 catcatcaat tacaacaaga gtaaaaacct tagaacttgg tgtaatacaa agatgtgcta
    58081 tgcatttagc ttcagatact gatttaaaac aagcagctga agttggtgca tatgggatag
    58141 aactatctaa aaaaggatat acaggagtta tggctatcat taatagaaca tgcaataatc
    58201 cttataagta tgaagttagt catactgata tttctaaaat agctaacaag acaaaatatt
    58261 ttcctaaaga gtggataaat gaagaatgta attatataac tgatgaagcc ctaaattatc
    58321 tatatccact tattcaaggt aaaccagata tcaaatataa aaatggacta ccttattttt
    58381 caattattta ctaggaaatt attataattt gatatacact caattctatc gctcttaaat
    58441 acctaagtgg ctgttaaata aaattataaa ttgttatatg acagtcactt aatcgtttaa
    58501 ttatttaact gtttagtttt ttaatttttt aatctttttt aatactaata cgatttgttg
    58561 taataatatc tcacacccat tttcattttg gatagattat aattagttag acaataaata
    58621 ttttgtcatg atattattat atatagtgat acaaatagaa atacttttat catgactaga
    58681 aaaacaaaga ttacacaaag gatttaaaaa tcaaacaact tcaattaaaa taaatggtta
    58741 aaataacttc taaatcaact acataaacaa taaaagaaca taaagtagtt aaatctacaa
    58801 ttataaaatt agtaagacta taataactta aataaaatta aagttaacaa agactattaa
    58861 agttagcaaa tattcctcta cctaaataat tgctttttat aaagagttat ttattttaat
    58921 ttattttaat tgaaatacga ttttaactag aacaatagat taatgtaata tttaatataa
    58981 aataattata ataattgaaa aggaagtaat ctatgatgaa aatagcagtc ataacaaccg
    59041 aatttttaaa agagtttgta gataattcta taaaaaagtt aaatattaat gcagaaattg
    59101 aaatatatat ctatagagat ttttctcatg ttggagattt atatttagaa atagaagata
    59161 ggtttgatgg atttgcagtt agtggcccca tccccaaaaa agctattaca aaaaaggctg
    59221 gaactattaa aaaacct
//
LOCUS       DAMKPG010000011        58712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMKPG010000011 DAMKPG010000000
VERSION     DAMKPG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.013"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            88..1284
                     /locus_tag="PC621_000756"
     CDS             88..1284
                     /locus_tag="PC621_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF4107942.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1377..1901
                     /locus_tag="PC621_000757"
     CDS             1377..1901
                     /locus_tag="PC621_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF4107943.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1926..2102
                     /gene="rpmF"
                     /locus_tag="PC621_000758"
     CDS             1926..2102
                     /gene="rpmF"
                     /locus_tag="PC621_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF4107944.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2309..2866
                     /gene="fapR"
                     /locus_tag="PC621_000759"
     CDS             2309..2866
                     /gene="fapR"
                     /locus_tag="PC621_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF4107945.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2877..3899
                     /gene="plsX"
                     /locus_tag="PC621_000760"
     CDS             2877..3899
                     /gene="plsX"
                     /locus_tag="PC621_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF4107946.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3925..4911
                     /locus_tag="PC621_000761"
     CDS             3925..4911
                     /locus_tag="PC621_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF4107947.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5020..5949
                     /gene="fabK"
                     /locus_tag="PC621_000762"
     CDS             5020..5949
                     /gene="fabK"
                     /locus_tag="PC621_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF4107948.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5965..6915
                     /gene="fabD"
                     /locus_tag="PC621_000763"
     CDS             5965..6915
                     /gene="fabD"
                     /locus_tag="PC621_000763"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF4107949.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6915..7664
                     /gene="fabG"
                     /locus_tag="PC621_000764"
     CDS             6915..7664
                     /gene="fabG"
                     /locus_tag="PC621_000764"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF4107950.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7718..7942
                     /gene="acpP"
                     /locus_tag="PC621_000765"
     CDS             7718..7942
                     /gene="acpP"
                     /locus_tag="PC621_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF4107951.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7971..9209
                     /gene="fabF"
                     /locus_tag="PC621_000766"
     CDS             7971..9209
                     /gene="fabF"
                     /locus_tag="PC621_000766"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF4107952.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9435..11153
                     /locus_tag="PC621_000767"
     CDS             9435..11153
                     /locus_tag="PC621_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF4107953.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11184..13184
                     /locus_tag="PC621_000768"
     CDS             11184..13184
                     /locus_tag="PC621_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4107954.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13532..13777
                     /locus_tag="PC621_000769"
     CDS             13532..13777
                     /locus_tag="PC621_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF4107955.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13812..14537
                     /locus_tag="PC621_000770"
     CDS             13812..14537
                     /locus_tag="PC621_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF4107956.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14559..15872
                     /locus_tag="PC621_000771"
     CDS             14559..15872
                     /locus_tag="PC621_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4107957.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            16171..16617
                     /locus_tag="PC621_000772"
     CDS             16171..16617
                     /locus_tag="PC621_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4107958.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            16706..18691
                     /locus_tag="PC621_000773"
     CDS             16706..18691
                     /locus_tag="PC621_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDF4107959.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18915..19883
                     /gene="spoIIIAA"
                     /locus_tag="PC621_000774"
     CDS             18915..19883
                     /gene="spoIIIAA"
                     /locus_tag="PC621_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF4107960.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19873..20394
                     /locus_tag="PC621_000775"
     CDS             19873..20394
                     /locus_tag="PC621_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF4107961.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20411..20608
                     /gene="spoIIIAC"
                     /locus_tag="PC621_000776"
     CDS             20411..20608
                     /gene="spoIIIAC"
                     /locus_tag="PC621_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF4107962.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20624..21010
                     /gene="spoIIIAD"
                     /locus_tag="PC621_000777"
     CDS             20624..21010
                     /gene="spoIIIAD"
                     /locus_tag="PC621_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF4107963.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21095..22300
                     /gene="spoIIIAE"
                     /locus_tag="PC621_000778"
     CDS             21095..22300
                     /gene="spoIIIAE"
                     /locus_tag="PC621_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF4107964.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22360..22977
                     /locus_tag="PC621_000779"
     CDS             22360..22977
                     /locus_tag="PC621_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF4107965.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22989..23543
                     /locus_tag="PC621_000780"
     CDS             22989..23543
                     /locus_tag="PC621_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF4107966.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23570..24259
                     /locus_tag="PC621_000781"
     CDS             23570..24259
                     /locus_tag="PC621_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF4107967.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24369..24731
                     /locus_tag="PC621_000782"
     CDS             24369..24731
                     /locus_tag="PC621_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF4107968.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24816..25322
                     /gene="nusB"
                     /locus_tag="PC621_000783"
     CDS             24816..25322
                     /gene="nusB"
                     /locus_tag="PC621_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF4107969.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25306..26376
                     /locus_tag="PC621_000784"
     CDS             25306..26376
                     /locus_tag="PC621_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF4107970.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26373..27578
                     /locus_tag="PC621_000785"
     CDS             26373..27578
                     /locus_tag="PC621_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF4107971.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27580..27795
                     /gene="xseB"
                     /locus_tag="PC621_000786"
     CDS             27580..27795
                     /gene="xseB"
                     /locus_tag="PC621_000786"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF4107972.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27798..28685
                     /locus_tag="PC621_000787"
     CDS             27798..28685
                     /locus_tag="PC621_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF4107973.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28715..29152
                     /locus_tag="PC621_000788"
     CDS             28715..29152
                     /locus_tag="PC621_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF4107974.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29186..31051
                     /locus_tag="PC621_000789"
     CDS             29186..31051
                     /locus_tag="PC621_000789"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF4107975.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31184..31996
                     /locus_tag="PC621_000790"
     CDS             31184..31996
                     /locus_tag="PC621_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDF4107976.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32016..33695
                     /gene="recN"
                     /locus_tag="PC621_000791"
     CDS             32016..33695
                     /gene="recN"
                     /locus_tag="PC621_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF4107977.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33910..34776
                     /locus_tag="PC621_000792"
     CDS             33910..34776
                     /locus_tag="PC621_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107978.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34867..35358)
                     /locus_tag="PC621_000793"
     CDS             complement(34867..35358)
                     /locus_tag="PC621_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4107979.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
     gene            complement(35419..35859)
                     /locus_tag="PC621_000794"
     CDS             complement(35419..35859)
                     /locus_tag="PC621_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4107980.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            36020..37078
                     /locus_tag="PC621_000795"
     CDS             36020..37078
                     /locus_tag="PC621_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDF4107981.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37220..38044
                     /gene="spo0A"
                     /locus_tag="PC621_000796"
     CDS             37220..38044
                     /gene="spo0A"
                     /locus_tag="PC621_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF4107982.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38457..39578
                     /locus_tag="PC621_000797"
     CDS             38457..39578
                     /locus_tag="PC621_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4107983.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39596..40471
                     /locus_tag="PC621_000798"
     CDS             39596..40471
                     /locus_tag="PC621_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF4107984.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40495..41187
                     /locus_tag="PC621_000799"
     CDS             40495..41187
                     /locus_tag="PC621_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4107985.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41195..42019
                     /locus_tag="PC621_000800"
     CDS             41195..42019
                     /locus_tag="PC621_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF4107986.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42024..43091
                     /locus_tag="PC621_000801"
     CDS             42024..43091
                     /locus_tag="PC621_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF4107987.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43093..43629
                     /locus_tag="PC621_000802"
     CDS             43093..43629
                     /locus_tag="PC621_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF4107988.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43697..44302
                     /locus_tag="PC621_000803"
     CDS             43697..44302
                     /locus_tag="PC621_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF4107989.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            44314..45228
                     /locus_tag="PC621_000804"
     CDS             44314..45228
                     /locus_tag="PC621_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF4107990.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45270..46442
                     /locus_tag="PC621_000805"
     CDS             45270..46442
                     /locus_tag="PC621_000805"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF4107991.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46489..47301
                     /locus_tag="PC621_000806"
     CDS             46489..47301
                     /locus_tag="PC621_000806"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF4107992.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47505..48830
                     /locus_tag="PC621_000807"
     CDS             47505..48830
                     /locus_tag="PC621_000807"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF4107993.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49043..50122
                     /gene="mltG"
                     /locus_tag="PC621_000808"
     CDS             49043..50122
                     /gene="mltG"
                     /locus_tag="PC621_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF4107994.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50294..50968
                     /locus_tag="PC621_000809"
     CDS             50294..50968
                     /locus_tag="PC621_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF4107995.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50955..52202
                     /locus_tag="PC621_000810"
     CDS             50955..52202
                     /locus_tag="PC621_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF4107996.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52274..53938
                     /locus_tag="PC621_000811"
     CDS             52274..53938
                     /locus_tag="PC621_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF4107997.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54035..54319)
                     /locus_tag="PC621_000812"
     CDS             complement(54035..54319)
                     /locus_tag="PC621_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4107998.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54342..55859)
                     /locus_tag="PC621_000813"
     CDS             complement(54342..55859)
                     /locus_tag="PC621_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4107999.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55984..56805)
                     /locus_tag="PC621_000814"
     CDS             complement(55984..56805)
                     /locus_tag="PC621_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF4108000.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            56996..58423
                     /locus_tag="PC621_000815"
     CDS             56996..58423
                     /locus_tag="PC621_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HDF4108001.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
BASE COUNT        23508 a         6103 c        10736 g        18365 t
ORIGIN      
        1 ataaaagata gagttttatt tagacattta ttacaaatca aaatttatta catattattt
       61 ataaaaaaat ctaaggagga aataataatg aaaatattag tattaaactg tgggagttct
      121 tcattaaaat atcaattaat agatatgaat aatgaagaag ttttatgtat aggattggtt
      181 gaaagaatag gaatagaagg ttctatatta aaacatgaaa aagcaggtag agatgataaa
      241 tacgttgttg aacaaccaat gaaggaccac aaagatgcaa tagcattagt tttagaagct
      301 gttgctcatc cagaatttgg tgcagttaaa gaaatgaaag aaatagatgc agtaggacat
      361 agagttgtac atgctggaga aaaatttgca acttcagtag taataactcc agaagtagaa
      421 gaagctttaa aagaatgtat agatttagct ccacttcata atccagcaaa cataatggga
      481 atagatgctt gtaaagctat acttccagat gtaccaatgg taggtgtatt tgatactgct
      541 ttccatcaaa caatgcctaa atcttcatac ttatatggtt tacctcatga gctatatact
      601 aaatatgggg taagaagata tggattccac ggaacttctc ataattatgt atctcagaga
      661 gcagcagaga tattaggaaa agatataaaa gatttaaaaa tagtaacttg tcacttagga
      721 aatggtgctt ctatagctgc tgttgatggt ggaaaatgtg tagatacatc tatgggattc
      781 actccattag aaggattaat aatgggaact agatgtggag atatagaccc agctatatta
      841 ccattcttaa tgagaaaaga aggtttagat gctgatggat tggataaatt aatgaataaa
      901 gaatctggag tatatggaat gactggaatt tctagtgact tcagagatat agaagatgca
      961 gctaaaaatg gagacgaaag agctcaggct acattagaag cgtatgttaa aaaagtacaa
     1021 aaatatatag gcgcttatgc tgctgaaatg aatggattgg atgttgtagt atttactgca
     1081 ggcgttggtg aaaatggaaa ggctataaga gcagatattg cttctaacat ggagttttta
     1141 ggaatgaaat tagataaaga agctaatgat gttagaggaa aagaaacagt aatatctact
     1201 gctgattcta aagtaaaaat gcttttaata ccaactaatg aagaattaat gatagctaga
     1261 gatacattaa gattggtaaa ataattaaat taaacctatg cctatcaagt aaaaatactt
     1321 gacaaacaaa tgctagattt gtataataaa tttggtgata tgttgaggtg aacttaatga
     1381 aagttagcat agaaaaaata aatagaaaag aaactgacaa aatagacttg aatttttgtg
     1441 aaaaaattga tactattagt tattgtgatg agatttacaa attagtatca cctgtaaatt
     1501 taaaagggaa agtgtcaaag actaataaag gtttgtatct agatatagat gtcaatttta
     1561 caattgttga caattgttca agatgtctta aagaagtaga aataccacta gagtattcta
     1621 tacaaggttt tttagtaaaa gaagaagatt atgatgaaga tgagtttgag gaatttgatc
     1681 cttttatatt cgatggtgaa gaaatcgacc ttattgatat aatagaacag acattagatt
     1741 ttaatgtacc tcataaagtt ctttgtagtg aaaactgtaa aggtctttgt caagtatgcg
     1801 gggcaaactt aaatgaagaa gagtgttctt gcagtgaaat tacaaacgat gaggaataca
     1861 tagatcctcg ttttgctaaa ttaaaagatt tatttaatta aattttgact aaggaggtgg
     1921 cgtaaatggc agtaccaaag cgtaaaacgt ctaaatcaaa cacaaaaatg agaagagcag
     1981 ctaactcaaa gatggaagca acaggatttg taagttgccc acaatgtcat gagccaaaat
     2041 taccacatag agtgtgtcca gactgtggat attataaagg taaagaagtt gtgtctaagt
     2101 aatagcttct aaaaagcatg tagagtatac tctacatgtt ttttttgtac atataaaaaa
     2161 ataagatgat ttactagaga aaataagtgg attttatgca tattaaaaaa tagaaaaata
     2221 ataatataag tattgaaaat ttttgtgtgt tactatatac tactattagt agctggtact
     2281 aattacagct ataaaatttg gagggaacat gaaaaagaaa agtaaagccc aaagacaaaa
     2341 agagcttata gatatgctaa agacagatcc tttttatact gatgaagaat tatcaagtct
     2401 gtttgatgta agtatacaga ccataagatt agatagaatg agtctaaaca taccagagct
     2461 aagagaaaga gtcaaatcaa tagcagaaac tcagagttca aaagtaaaga ctttaggagt
     2521 aaaggaaatt acaggagaaa tcattgattt atctgtaggg cgattaggta tatctatgtt
     2581 agaagttaca caggacatga tttactctaa gactaatact ttaaaagata cgtatatttt
     2641 ttctctagca gattcattgg caatggctat aatagatgca cctaaggtta ttatgagagt
     2701 tgcaaatgta aaaagtttta agttgataga acaacaagat agacttatag caaaagcaga
     2761 agtgtataga aatatagata aaaagcatta tgtaaaagtt gttatcaaca ataaagccca
     2821 agagcaaata tttagaggaa agtttatctt tgaagaatta gattaggagg gaaattatga
     2881 aaatagtaat tgatggaatg ggtggagata atgcacctaa atctaatgta gaaggcgcag
     2941 taaatgctat aaaagaatat caggttgatt taataatcac aggagataaa gatttacttg
     3001 aaaaggaatt ttcaaattat gaatttgata gaaataaatt agagatagtt catactactg
     3061 agattataga aaacgaagat aaaccagtaa aggctataag aagtaaaaaa gattcatcta
     3121 tggtagtagc attaaatctt gtaaaagaag gaaaggcaga tgctataata tcagctggaa
     3181 atacaggagc cttacttgct ggtggattat ttgttgttgg tagaataaaa ggaatcgata
     3241 gaccttgctt atgttcagca ataccaaatg taaagcgagg aatgacactt atagcagact
     3301 gtggcgcaaa tgcagattgt aaacctaaga atctagttga atttgcagct atgagtaata
     3361 tttattcaag aaaagtttta ggtcttgaaa atcctaaggt agcattagca aatgttggac
     3421 tagaagaagg caaaggcaat gatttagtca aaaggtcata tgaagaaatt aaaaaattag
     3481 atttaaattt tatagggaat gtagaagcta gagaagttat aaatgcatac acagatataa
     3541 tcatatgtga tggatttaca ggtaatatat tacttaaatc tgctgaaggt gtagcacttt
     3601 cagttatgag tcttataaag gaaactttta tggccagtac aaaaagtaaa ataggagcat
     3661 tacttataaa ggatgattta agaaaattaa aaagttttat agattattct gagtatgggg
     3721 gagctccact tttaggtcta aatggaggag ttataaaagc tcatggtagt tctgatgcta
     3781 aagctataaa aaatgctata aatcaaggaa taaaattttc aaagggaaaa gtcgttgaag
     3841 atattaacca atttataagc aaatacaatg aagaaaataa aaataacgaa gatgaataaa
     3901 ctcgttttgg aggtttaata tataatgaat acaaaggctg gaatattagg agtaggaagt
     3961 tatctacctg agcaatcata tgataatttt cacttcgaaa aaataatgga tacatcagat
     4021 gagtggataa gtactagaac tggtattaag gaaagaagat ttgccaaaga gtctgaagca
     4081 acttctgact tagcatctaa agctgcctta aaagctatag aatgtgcaaa gttaaacgta
     4141 gaggatatag agctgattat attagctaca attactcctg atatgtcttt gccttcaact
     4201 gcatgtatag tacaagatgc aataggagca gtgaatgcta ctgcatttga tatatcagca
     4261 gcttgttctg gatttgtata tggagtaact attgcaaagc aatttgtgga aacaggttgc
     4321 tataaaaatg tgcttgtaat aggagcagaa acttgttcta agtttttaaa ttatgatgat
     4381 agaacaacag cagtattgtt tggagatgga gctggagcag ctgttattgg tcctgtaaat
     4441 gagggtggaa tactgtctac acatatgggc tctgatggta aaggaaaaga ttgtttaaaa
     4501 gtacctgctg gaggttcaag acttaaggca agtaaagaga ctgtagaagc taatctacat
     4561 acaatagaaa tggcaggtag tgatgtgttt aaatttgcag ttagaaagat ggcagaaact
     4621 tctttaagag ctcttgaaaa agcaaaccta aatacaactg atatagacta cttagtgcca
     4681 catcaagcta atataagaat catacaagct tcttctaaga gattagagct tgatatgaaa
     4741 aaagtttatg taaatataga caaatatgga aatatgtctg ctgcatctat accagtagca
     4801 ttagatgaag cttatagaga aggtaaaatt aaaaagggtg ataatgtagt tttagttggc
     4861 tttggtggag gtctaacttg gggggcttca gttgtcaagt ggactttata gtataaaata
     4921 tctaaaatga tttaattaac aaaattaaac ttatacacaa aataaagttg attaataata
     4981 aaaagtttac ttttatgatt tcaagtgaag gagaattgta tgaataaaat ttgcaaaata
     5041 ttaaatataa aatacccagt tatccaaggg ggaatggcat gggtagctac tgcatcatta
     5101 gcaagtgctg tatctaatgc aggaggactt ggcataatag cagcaggaaa cgcaccaaaa
     5161 gaagctataa agaaagaaat tgttgagtgt aaaaaattaa cagataaacc ttttggagta
     5221 aatgtaatgc ttatgtcgcc atttgttgat gatataattg atttgattat agaagaaaaa
     5281 gttcaagtta ttactactgg tgctggaaat cctgcaaaat atatggatag attaaaggaa
     5341 gctggaacaa aggttattcc tgtagtacct acaatagctt tggcacaaag aatggaaaag
     5401 ttaggagcta cagcagtaat agcagaaggt actgaaggtg gaggacatat aggagaactt
     5461 actactatgg tcttagttcc acaagttgct gatgctgtaa acatacctgt aatagctgct
     5521 ggaggaattg tagatggtag aggaattgca gcatcatttg cattaggtgc cagtgcagtt
     5581 caagtaggaa ctagatttat ttgcagtgaa gagtgttctg tgcattcaaa ctataaaaac
     5641 ttagtactaa aagcaaaaga tagagatgca attgtaacag gaagaagtac tggtcatcca
     5701 gtaagaacat taaaaaataa actatcaaaa gaatttttaa agatggaaca aaatggagct
     5761 actcctgaag aattggataa aaaaggtaca ggagctttaa gatttgcaac agtagatgga
     5821 gacatagaaa aaggttcatt tatggcaggt caaagtgctg ctatggtaaa agaaataaca
     5881 ccttgtaagg aaattataga ggctatggta aatcaagcaa gagagattat gccagcaata
     5941 gaactgtaaa tcaggaggag caagatggga aaagttgcac tagtattccc tggtcaagga
     6001 gctcaatatg taggtatggc caaggattta tatgaaaata atgctgttgc taaaagtgta
     6061 atagatgagg ctagtgatgc cttaaatatg gatttaaaaa accttatgtt taatggaaat
     6121 gaagaagaac tttctaaaac tgaaaacact caacctgcta tggttacaca tagtgtagca
     6181 gtattaaaag cggtacaagc tcaaatagat ttaaaatata atgcatgctt aggattatcc
     6241 ttaggagagt atagtgcgtt ggttgcagca gatgcaattg actttaaaga tgctgtatgt
     6301 ttagttaaaa aaagaggtaa gtttatgcag gaaacagttc cagcaggtgt tggagctatg
     6361 gcagcaattt taggtttaga tagaaatgta cttgaaaatg tggttaaaga tatacaagga
     6421 ggtattgtag aagtagcaaa ttacaattct cctggacaaa tagttatatc tggtgaaaat
     6481 gaaaagatag aagaagcaat ggtaaaatgc aaagaagcag gagcaaaaag ggcagttaaa
     6541 ttaaatgtaa gtggaccttt tcactcatca atgttaaaag aagctggtgt aaaattggcc
     6601 agtgaattag aaaaagttaa cataactaaa cctaaagttg atgtggtggc aaatgtaaat
     6661 gcagattact ataaaaacga aggtaaagac ctgcttataa aacaagtaag ttcatctgtt
     6721 ctttgggaag attctataga aatactttta aatgatggat atgatacttt tatagaaatg
     6781 ggacctggaa aaactttaaa agcatttatt aagaagatag cttccaataa aaaagcaaat
     6841 gtcaatattt ataatataga tggtatagat tctcttaatg aatttgtaca aaattataga
     6901 aatggagaga tataatgata aatcttacag gacaagtagc agttgttact ggtggctcta
     6961 ggggtatagg aaaagaaata gcaaaaaaac tagcatcttt tggagctgat gtagtaatca
     7021 attatacttc taaagaagat gaagcactaa aaactaaaaa tgaaatagaa agtatggggg
     7081 taaagtgtac ctctataaaa tgtgatgtgt ctaaatttga tgaagtgaat caaatgatag
     7141 attctgttgt aagcgaattt ggaaaaattg atatattggt taataatgca ggcataacta
     7201 aagatggtct gcttatgaga atgaaagaag aagattttga tagagttata gatataaact
     7261 taaaaggtgt ctttaattgt acaaaagcag ttactaaacc tatgatgaaa aagaagtatg
     7321 gaagaataat aaatatgact tcagtagttg gaattatggg taatgcaggg caaactaatt
     7381 attgtgcatc aaaagcaggt gtaattggat ttacaaaagc ttctgcaaga gagttagcat
     7441 caagaaacat aaatataaat gcagtagcac ctggatttat agaaacagat atgactagag
     7501 tactaagtga tgatgtaaaa gaatcaacac tagcaaacat accaaagaaa tcttatggta
     7561 aaccagaaga tgtagccaat gccgtagcat ttttagttag tgacatgtca agttatataa
     7621 caggacaagt aataaatgta gatggtggaa tggtaatgca ataataattt aaaaagtaga
     7681 gaaatctatt aaaataaaaa attttaggag gtacacaatg tttaataaag ttgtagaaat
     7741 aataatagag caattaggag tagaagataa agaaataact atggagacat cattaatgaa
     7801 ggatttagaa gcagattcat tagatgcagt agaaataata atggcacttg aagatgaatt
     7861 tggtatagaa ataccagata cagaagcaga aaacttcaaa tctataggag atatagttaa
     7921 gtatatagaa gctaataaat agtcgagctt taaaaaagtg gaggtatccg atgaataaaa
     7981 gagtagtaat aacaggactt ggatgtgtta cgccgttggg aacgggaaaa gaagagttct
     8041 ggagtaatat aaaatctgga gtatctggta tagataaaat aactaatttt gatgctagca
     8101 catatcaaac tcagatagca ggagaagtta agaattttca tccagaagaa tatataagta
     8161 aaaaagaatt gaagagatta gataaatttg ctcaatttgc aatagtatct gctaaattag
     8221 cagttgaaga tgcaaatctt gatttagata aagttgatag agaacgtttt ggtgttataa
     8281 taggttctgg aataggtgga gttgaagcta tagaaactca acataaaatt cttttggaaa
     8341 aaggaaataa aagagtaagt tcactttttg tacctatgat gataggaaat atggcagctg
     8401 gtcaagtttc aatattttta ggagcaaaag ggcctaacac taatgtatgt acagcttgtg
     8461 catcaggaac tcattcaata ggtgatgctt ttaaggtaat tcaaagaggt gatgcagaca
     8521 tcatggtagc agggggctct gaagctgctg taacaggtct tgcttttgct ggattctgta
     8581 acatgaaggc tatgtcaact agaaatgatg accctaaaac tgcatcaaga ccgtttgata
     8641 aagacagaga tggttttgta atgggagaag gagcaggtat tgttatctta gaagacttag
     8701 aacatgcttt agcaagaggg gctaaaatat atgcagaagt agttggatat ggtttaactg
     8761 ctgatgcata ccatatgaca actccagctg aaaatggaga gggagcagct aggtctatga
     8821 atatggcttt gaaagatgga aatgttccat tagaagaagt agattatata aatgctcatg
     8881 gaacttcaac ttattacaat gatttatatg aaactatggc tataaaaact gtatttggag
     8941 aaaaggcata tgacttatgt gtgtcatcaa caaaatctat gactggtcac ttattaggag
     9001 catcaggtgc tatagaggct gttgtttgtg ctatgagtat agaagatagt tttgtgcctc
     9061 caactataaa catacaagaa gttggtgaag atttggattt agattatgtt ccaaatcaag
     9121 gtaaagaaaa aaatattaga tatgcactat caaattcttt aggatttggt gggcataatg
     9181 ctacaatagt actaaaaaaa tatgtataaa taaatattaa atataaaact aaaaaacaag
     9241 cacttgtttt aactgtataa atttatatta gcttaaattt aaagtctcct aaattatttt
     9301 aggagacttt tttattaaga tttaagtcta gcatatgttg taaatcattt tgatatattg
     9361 tattattaat tattaagagg ttatttatat atgcaagttt tcattctaca atatacaaaa
     9421 ggaggactaa aattttgaat tttagactac caaagaaaag gttacccaaa aagataattt
     9481 ttatatgtat aatacttatt agtattattt tgtttataaa gtatgaccaa aatggattga
     9541 aagaaagaga aattataaat aagggaaaat atatgtctga aagccttgtg gcagataagg
     9601 agtatgatga agcaaaaaaa gtggttaaga cttctttcga atctattcca gaaaaaaaat
     9661 acaataaagt atataatgaa gtttgggata tgttaaagac tatttcatat gttccagatg
     9721 gaacgaagat agtcattgat tctttagaaa agatgagaga atcagatgtg aaattatctg
     9781 atgaatgtag atttaatata gaaaaaagat tagcatcagt gtatataatg gataataatt
     9841 attctaaggc agtagattta actgtaaagt ctataaaatt ggctgagaaa ttggggaata
     9901 attatgaaaa agcaagacta gatgttgatt tggcgagcat atttttaaat ataggtagct
     9961 atgaaacagg agagaaatta ataaaggatt cttttaaaat agatattgat gaaggtgaaa
    10021 aaaatggaat ggttagatta tatgctatta taaatctagc agaaatatat gtagaagagg
    10081 gagaatatga taaagctatt gaaataagta gcaggataaa agattataaa aagtttgtaa
    10141 atacggataa ttacaatgat tttgatataa tggcctctat aatgaagtca aatgcgtatg
    10201 tgggcaaaaa taatatacaa aaggcaaaga cttttttaga aaaagctgat tatcttataa
    10261 aatcagatag aacagtttat atattagata aagatatgta ctattatatg gcaatgggaa
    10321 acttagaata taaaagcgaa aattataatt ctgcaataga taattataaa aaaagtttag
    10381 agatatctac caaaagaaag ttaactcagg agaaaataaa aaatttaaat aagatattaa
    10441 atatatatga aaaacaagga aatatagaat ctttaaatga gtatcaaaag atgttaataa
    10501 atgaatataa agagaacaaa agtataagag attcagagac tagtttctat ataattgata
    10561 aagtatcaaa cgaaagtaag ctatttgaaa aaactaaaaa agaaataaaa tattataaaa
    10621 tattatttac agtaatttta attgctatat tagggtctac ttttttatac aaccgattga
    10681 agtattttaa agcacaaaat ttacatgatg gtcttacaaa tgtttataat agaaaaagtt
    10741 ttgatattat gtatgaaaag tacagagaga aagatgataa ttttgcactt gtgatgatag
    10801 atattgataa tttcaagctc ataaatgata attatggaca taaatttggt gacactgtta
    10861 taaagggaat aacaaatacc atttgtgtta tgttagaaca agatgataaa gtctttaggt
    10921 acggaggaga agaattttct gtattaataa gaaataaatc tggtgaggaa gtagcagata
    10981 taattgacaa tataagggtt gcaattgcaa ataaaaagtg gaatgatgat gtgactgtga
    11041 ctataagtgc aggtgtagct catatttcag atagcaaaga tgtactagaa gaagctgata
    11101 aaaatttata taaagcaaaa caacttggta gaaataaagt agtatataag taattatata
    11161 ctatataaat aagaggtata aatatgaaac aatcaatagc tatagtaact gataaaataa
    11221 ctaaactatc atctttctta gaagaaaata tacgtttggt tttaggtaat tatattgata
    11281 taaaccacta ttatctggaa gttttaaaag agaatgataa aatacaaggt gattttgtac
    11341 tagtaatgaa tgatgataga ttaaacagta tgagaaagca tttagtacaa gatagtaaga
    11401 ttatagtgat aaggagaaca cttaaagaaa atgaaatata tggattattt tcaataccaa
    11461 atggaactga agtattggtt gtaaatgata caaaagaaac taccttagaa acgataagct
    11521 tattttacaa aataggagta aaaaatttaa agtttactcc atatattgaa ggaaaaattt
    11581 ataaaaatat agacatagct ataacgcctg gagtaaagga aaaagtacca agttatataa
    11641 ataaagtaat cgaccttggt aatagatata ttgatatatc tacttttata gaaataataa
    11701 atggtcttag aattgatgtt aaagaaatac gtaaaaattt aatggcatat tctgagagtt
    11761 taataagttt agataatgga gttaaagata attataggca actgttttta aaaatagaag
    11821 aacaagatgt tatattgaat ctatcaaaag atggaataat atttacttca gcagatggta
    11881 taataaacac atttaataaa gaagctttaa aaattttagg tataaatgaa aatccacaag
    11941 gcaaaaacat agaagatatc atttctgaag atttaaatat tttacttgaa gacaaagaaa
    12001 taattgatga agtggtattt acaaataaaa aatatataaa tgtaaataaa aaaagtattt
    12061 tttctatggg gaataaagct gggatatatt ataacttaca agaaataacg tatataaaaa
    12121 aattagaaca aaatcttact aataaattaa gagaacaagg tcagatagca agatatacat
    12181 ttaaagatat aaaaactaaa aataaaaata tggtaaagtg tgtagaattg gctaaaaaaa
    12241 tatctaaatc agacctttct gtattaataa taggagaaag tggtactggt aaagaattat
    12301 tatcacaatc aattcataat gcttccaata gaagtaatca accatttata gcggttaatt
    12361 gtgcagcagt tccagatagt ctattagaaa gtcaactgtt tggatatgaa aaaggctctt
    12421 ttacaggagc tttaagagaa ggaaaaaagg gtctatttga acttgctaat aatggaacta
    12481 tatttctgga tgaaattgga gatatgcctc cattgctaca gacaaagtta ctaagggtat
    12541 tacaagaaaa gcaggttatg cctataggtt cccataatgt tataaacata gatgttaggg
    12601 tcatagcagc aacaaacaaa aacttactaa agatgattag agaaggaaaa tttagagagg
    12661 acctgtatta tagactaaat gtattgccaa tagatgtacc atcattgagg aatagaaaag
    12721 aagatataat aacacttatg aatttctttt tgaaaaacaa tgtaagactt tcgccagaag
    12781 ttataactat tttagaaaaa tataattggc caggaaatat aagagagtta caaaatgttg
    12841 cttcttatgt aagtctaatg tgtgatagta tggtttttag agatgactta ccaccatata
    12901 taagggtaga atgtgctaac aattatgaag atggatttgg ttatacaaaa aaagaatttg
    12961 aggaaatact gaggattcta tatgaaagaa gagagtcatc ctctggagtt ggaagagggt
    13021 ttataatcga aaagcttata caggaaggga ttgagatttc tgaaagcaaa gttagaaagg
    13081 tgctatctta tttaagtgaa tgtggatata tatcatctaa atctggtaga ggaggaagtc
    13141 aaataacctc agaggggata aaactttata ataatataaa ttaagctaag tatttgatat
    13201 tgaaatcaca tattttgaat ttagatattt aaataataaa cttttatatt tatagagatt
    13261 aatttatact atgtcagaaa gaatattgta atttataatt aaatattaat aggtttttaa
    13321 aataggatta gataggtaat aggttaagtt agaataaccc attacctatt tttatttgta
    13381 agaaaagtgt acatagagtt aaaagtaaaa aatacaacta ataaaactat tgaaaacact
    13441 agataaataa taaataaaaa taattataaa ttttggcata tatattgcta atcatagaga
    13501 taagacgaca aaataattgg gaggtttaat gatgaaatac aaaacaacag ggacttgtgc
    13561 tacagaaatt gaatttgaag taaaagaaaa taaagttaca aatgtaaact ttataggtgg
    13621 atgtgatgga aatttaaaag ggctaaaagt attagttgaa ggcatgaata tagaagatgt
    13681 aatacaaaag ttaaaaggaa tagagtgtaa aacaaagcca acatcttgcc cagaccaact
    13741 gtctctagca ttggaaaatt atatgaatat aaagtaatac ataaaacaat cgattagtta
    13801 ggagtttaat tatgaaacca ataataggga ttttaggaaa tttaataata atggaaaatg
    13861 gtatgtttcc aggtcttgaa aggtcatatg taaataatga ttatataaat gcagtgctaa
    13921 aaggtggcgg tagtccagta ataattcctg taaatacaga taaagaagtt ataaaaaaac
    13981 agattgaaat ggttgatgga gtcttaatat ctgggggatg ggatatcaat cctcaactgt
    14041 atggagaaga aacaagagaa gaaacgacat ttatatatcc agaagtggat gaatttgatt
    14101 taattgcaat aagtatagca ttagaactta aaaagcctat attaggtgta tgtaggggac
    14161 tacaaatttt aaatgtaagt ttaggaggaa ctttatatca agataataat cttatagaag
    14221 gaagttatat aaaacataca caatcttcta aaagacatgt agctactcat aaagtcgatg
    14281 ttaaagaagg aagtatttta gagggaattt taggaaaaca attacttaca aatagttatc
    14341 atcatcaaag tgtcaatcag cttggaaaag gattaaaagc catagcatac tcaaaagatg
    14401 gtataataga agctattgag aaagaggagg aaaattttgt agtaggagta cagtggcatc
    14461 cagaaatgat ggtagattac tgtgataaaa tggaaaaact atttaaatat tttataaata
    14521 tttgttcaaa tttataatat accttggggg gattgaacat gaacgaaaag aacaaaatgg
    14581 gattaataag tattatactt ttaggtatta atgcagtagt aggagctgga gtttttctat
    14641 taccaggaga tgcaatgaaa tcatttggtg tagcaagtat atttgtatat atatttgata
    14701 tgttgttagt tttatctatg gcattctgtt ttgctgaggt tgcaggtaaa ttcaataaaa
    14761 atggcgctgc ctatgtatat accaaagaag cttttggaga tttttgtggt tttgaagtag
    14821 gtcttatgaa atgggtaata ggatgtattt cttggggagc tttaatagta ggttttccta
    14881 catcattatc agcagtatgg gctccagcag gtgaaccaca tatacaaaaa attataattg
    14941 tagccatgat agttgggctt acaattataa acttattagg tgtatctcta tcaaaaattg
    15001 tacaaaatgt tataactgta ggtaaactaa ttccattaat actatttata ggaataggta
    15061 tattttttat aaaaggagtt aattttactt cttcaacaat ggtacctcca ggagctggag
    15121 caacagagtt tggagcagca gcacttttaa tgttttattc atttacagga tttgaatcta
    15181 tagcagttgc agcagaagat atggaaaatc cacaaaaaaa tataccaata gcaattataa
    15241 gtgtaatagt aattgcatca ataatatata tattaaatca agttgtatgt gttggtatac
    15301 taggagattc gttatctagt acatctacac cagtggcaga tgctgcaaga atatgctttg
    15361 gaaatatggg agctggattg gttacctttg gaacattggt ttctgttggt ggaatttgta
    15421 tgtgtggtgc ttttgtaaat ccaagaagtt gtgtagcttt agctgatgat aaaatgttac
    15481 caagaatatt tgcaagaaaa gataaaaaag gtacaccata tgtagcgata atagctacta
    15541 tgttaatcac tataccaata gctctatctg gaagttttgc tgaattagca gcaataagtg
    15601 cagttgctag attcatacaa tacataccaa catctttatc cgtattagtg tttagaaaga
    15661 aaagaccaga attagtaggt acttttaaaa ctccttttgg agcagtgatt cctctaattg
    15721 ctgtatgtgt tggaacatgg ttgttattcc aagcatctat gcatcaatta attatgggat
    15781 taggagcttt ggcaattgga gtaccattat actttataat gaagtcttat aatagaaaag
    15841 tctgtggtga aaatttaaag aaaatagttt aattatgtta taagaattta ttgagttaaa
    15901 attagacatt ggcagaaaat taaatacaaa taagtatcta taaggctata ttaaaatatt
    15961 attaattttt tattttaata tagccttata ttgttatcaa taaatgaaaa atattgtaac
    16021 aacagaagaa caatagtatt atgtttatag ataaaaaact tattaggagg taaatttatg
    16081 ccaacaataa cattaaaaaa tggtgtagac gtacttatta gagaaggtgt aagagaagat
    16141 gcttaaagca ttatagattt ttataatgaa gtgggtggag aaactcattt tctgtcattt
    16201 ggaaaggatg aatataaaat atctttggag gaacaagaaa atgctataga atcagcaaaa
    16261 gcatctgata attctgttaa actgattgca tttatagatg atgaaatagt tgggattgca
    16321 acgattgatt ctaatcaaaa agcaaaagga aagcatgttg gagtattagg aattgttgta
    16381 aaagaaaaat attggggaat tggattgggt aaaaggctta tgttagacct tatagaatgg
    16441 tgtaaatcaa atggaataac caagaagata actcttgtca ctaatgaaga aaattataat
    16501 gctataggat tatacaaaaa agttggattt gaagtagaaa gcatactaaa aaaagaatgt
    16561 tattataatg gtgtttatac tgatttaata ggaatgtcac ttttattggg aatataatta
    16621 gtgtacattt tatctcgaaa aaagtatatt aagtatggta taattaaaaa agaatataac
    16681 ttaatataat tttggaggac gaattatgaa aaatctgtgt aaaatatcag atgattattt
    16741 aaacagcaag cttaagtatt taaagttatt atcaaaacaa tatccaagta tatcaaaagc
    16801 aagtacagaa ataataaatt tagaggctat attaaatctt ccaaagggaa cagagcattt
    16861 tataacagat gtacatggtg aatatgaacc atttgtacat gtattaaaaa atggttctgg
    16921 tgtaataaag agaaaaatag aagaattgtt ttcaaataca ataagagaca gcgaaaaaaa
    16981 aatgttagca actcttgtat attatcctga acaaaagtta gacctaataa ttaagcaaga
    17041 ggaaaatata gatgattttt atagaataaa tatttataga cttatagaac tttgtaaata
    17101 tgcttctagc aaatatacta ggtctaaagt tagaaaactg ttaccagaaa attttaaata
    17161 cataatagaa gaacttttgc atgagcatgt taaaagtgaa cataaagaag agtattataa
    17221 aagtatagtt gaaacaatag ttgacatagg aatagctaag gagtttataa ttgctatttc
    17281 tacagttata caaaaattgg ttgtagatag acttcatgta ataggagata tttatgatag
    17341 aggtccaaga ccagatataa ttgtcgataa gcttattgaa catcattgtg tggatattca
    17401 atggggaaat catgatatat tgtggatggg tgcagcctca ggtgaaaaaa cttgtatagc
    17461 aaatgcactg aggatatcag ctagatatgc gaatttagac attgtagaag acatatatgg
    17521 aataaatctg ttgcctttag ccacttttgc tatagaaatg tataaagatg acccttgtaa
    17581 agaatttatt ccaaaggtta atgaccaaag tgtaacaaca acagaaaagt ctttaatggc
    17641 aaaaatgcac aaagcaataa gtataattca atttaagctt gaaggtgagg ttataagacg
    17701 aagacctgaa tttgaaatgg aacatagact tttgcttaat atgataaact atgatgaagg
    17761 tacgattact ttaaaaggta aaacatataa attaaaagat acttatttac ctacaataga
    17821 caaaaaagac ccatataaat taactatgga agaaagaaat gttattgata aattagtatc
    17881 ttcatttaga ggtagtgaaa aattacaaaa acatgtttca tttttgtttt ctaaaggaag
    17941 tatatattta aaggctaact caaatttact gattcatgga tgtgtaccat taaatgaaga
    18001 tggaagcttt atgtctatga atataatggg aaaagagtat aaaggtaagg ctcttatgga
    18061 taagatggag tctctagcaa gagaaggatt tttctttaaa gataaggcag aagaaaaact
    18121 atatggtatg gatattatgt ggtatttatg gactgggaaa tgttcatcat tatttggtaa
    18181 agatgatatg actacatttg aaagatactt tatagaagaa aaagagactc ataaagaaaa
    18241 taagaatcca tattttaaac ttagagaaaa tgagatggct tgtaaaagat tatttgaaga
    18301 atttgattta gagcttgatg aatcccatat aataaatggt catgttcctg tggagagtaa
    18361 aaatggagag agcccaataa aagctaatgg taaaattctc gttatagatg gtggtttttc
    18421 tagggcttac caaaaaacta ctggtatagc tggatacact ttgatataca attctagaac
    18481 tttgcaatta gtatcgcatg aaccatttaa ttctgctgaa gaagccattg caaatgaaag
    18541 tgatatctta tcgacaactg ttgtagtaga gcataaggca aagagaaaaa tggtgagaga
    18601 tactgatgaa ggagtaaaaa tacaagagga aatagaagat ttaaaactac ttttaatggc
    18661 ttataagaaa gggctaataa aagaaatgta gatattatag aggcacttca taattgaagt
    18721 gtcttcttgt taatacttat taaatatatt ataaagatta ataatagtag tttatattat
    18781 acttactaag tgaatccaag ataaatgggt tcactttttt tatttataaa cattattaaa
    18841 aaaatataga tgttttggtt atattccttg tcctttataa atagaattat ataaacaaag
    18901 gaggaggaca agagatgaat aaactttctg atgaaataat aaactctctt tctacaacta
    18961 taagagaaaa ggtagaaaaa gtctcaaaca ataatctgaa catagaagaa attaggttac
    19021 gctcacaaaa accattgata ctaaatgcaa actctaaaga ttatttttac aatcaaaaaa
    19081 ctatgacact tgatttaaat caacaaaact catatgtagt aacaagagaa gatgtagagc
    19141 aaacttttca aataatatgt aaatattcaa tacattcatt tatggatgac ataaaaaaag
    19201 gattcataac tttgagagga ggtcatagag tagggttagt tggaaaagca atagtagaag
    19261 atggacaagt caaaaatata aagcatatat catctttaaa tataagggta tcaagggaaa
    19321 ttattggatg ctcggataag attttaagtc atattataaa gggaaaaaat cagattaata
    19381 atactttgat aatatcacca cctcaatgtg gaaaaacaac tttaataaga gatatagtaa
    19441 gaaatttaag taatggtaat gaagattatg gatttaaagg tttaaaagta gctttggtag
    19501 atgagcgtaa tgagatagca ggagcttatc taggtgttcc acaaatggat gtgggaataa
    19561 gaactgatat aatagaaact tgcccaaaag atttgggtat tacgatgctt ttaaggtcta
    19621 tgtcaccaaa tgttattgtg acagatgaaa ttgggagtga aaaagaaata aaggcattat
    19681 acacagcatt aaatggaggt ataggattaa taactactgt gcatggagat tcaatagatg
    19741 atatacaaaa tagaaaagaa ttaaatagac ttctggataa agagttattt aaaaaggtta
    19801 taattctttc tgcaaaaaga ggagcaggaa cgattgaaaa aatttatgat ttagaagaaa
    19861 agagatggta ttttgcaaat taaaataatt attatagctc tcttggtagg aagtagttat
    19921 ttgataggtg aacaaatata caaaacttat actcgtagac ataaacaatt aaacgattta
    19981 ataagagttt tagaaatttt gaggatggat ttatctttcg gactttatac attagaagaa
    20041 atctttaata ggataggtgg aaataaagag ttttgttttt ggaaattttt ctatcaaata
    20101 tcagaaggac ttcacaatga acaaagtaaa actttggaaa taataatatc agaaaatatt
    20161 gatgtcttaa gtaaagaaac ttatctaggc aataaagaaa ttgaggaact gaaaaatcta
    20221 atacttactt tagggaaaag cgatatagag tcacaacaaa gaatgataga tttatctata
    20281 gaaaatttga aaaaacaaac ctatgagaca aaggaagata taaacaaaaa aggcgtattg
    20341 tataaaaaat tagtgacatt cataggtata ggtatatgta ttatattgat ttaatatggg
    20401 gaggtttatt atggaaatat cattgatatt aaaggttgca ggtgtcggta ttctaatatc
    20461 agtattgaat atgatattgg aaaaaacaga tagaaaagat tgggcaggtc ttactacatt
    20521 agcaggtgta ataatcgtat tgggtatggt aataacagaa ataagtgact tgtttaatac
    20581 tgtaagaaca atgtttcaac tttactaaaa aggagggagt aatttggaaa taatgcaatt
    20641 aataggaatt gcaatcattt caactacact ctgtttagta attaaaaaag atagaccaga
    20701 gatagcaaat ttcatagcaa taataactgg agtaataata ctattgtcag ttatgtttaa
    20761 gctaaatttt atagtagata gtatacaaga tttggctaac aaggctaaca taccgactat
    20821 gtatatatca ttaataataa aattaattgg gatagcatac ttaatggaat ttgcgataca
    20881 actttgtaaa gattgtgggg aaggaaacat tgcttcaaaa ctagaatttg gaggtaaaat
    20941 aatagttatg tcaatgtctt tccctatact tttgtccatt gtagaaatgg tggtgaatat
    21001 aattccatag atgtgtatct tggaatgaat ataagtatat tagagaactt tcgttgtatt
    21061 gaggtttata gagtgaagag aggcatggtt gcttatgaaa aaaagatttt taccaatgat
    21121 tataggtttt ttatttactt tcttttttgt aaatatcttt gctattatga tatttgctaa
    21181 tgaagtacca agtagtgaag ataaagaata tggtgaaaca aaaaatagca tagataaata
    21241 tatagatggt caattagaca agctagatat aaatgagatt caagactata taaataaaga
    21301 aatagttatt aatgatgtta atttaaaatc atttgtaaaa gatttaatta gtggagaaaa
    21361 aaatatctta gatttattca ataaagatgg attgaagata ttgatgtttg atgaatttaa
    21421 agcaagttta aaggtagtag cagtaatttt ggtactggct ctgttatcat ctattttaaa
    21481 gagtctggaa aactcttttt catcaggtgc agtaagtcaa atagcaacat atataatatt
    21541 tatcacaatg gtatcactta cacttattgg ttttaaagat gtattacaga tttgttatga
    21601 tgcaatagac catactgtag gtcttatgca agttataatg ccaatattaa taacattttt
    21661 acttttaatt gggttcccaa taacgtcgac tactttgaat cctatcttca taggaggagt
    21721 aacatttata aatgtatttt ttaaaaattt tctatttgta tctattactg ttgcatttgg
    21781 aattttaata ataaacaatt tatcaaaaaa tataaggcta aagagatttt tctcctttgt
    21841 aaaacaaata aattatgttt ctattggagc tatgtttact gtttacttag gtcttgtatc
    21901 catacaagga ttgtatgtaa ccagttttga taaattcagt gtaaagactg ctaaatttgc
    21961 tataggtaat tttattcctg ttgtaggtgg atttgtatct gactctgtgg atattttatt
    22021 atcttcatct cagcttataa aaaatatatt tggaggaata gggcttatct tacttgttgg
    22081 aatttgttta cttccagtaa taaaaatttt atcagtaata gtagtgtaca aattagcagc
    22141 aataattgta gagcctgttg gagaggatgg aatatctaat tttttaaatg aagtggcaaa
    22201 tttaatgata ataatgctag catcagtaat tgctataaca gtaatgttct ttgtaactgt
    22261 agctatcttg acctcgataa gtgtcgtaag tcaagggtaa tttatatatc agtgaaaaat
    22321 aattacattt aatctaatgt ttttagagag gaggatgcta tgttagaggg cataaaggca
    22381 tggatagtta gtgttttgat aggagcattt atagtaaata tagtagatat gattttacca
    22441 agttctaaga taaaacctta tgtcaaccta gttttaaact ttatgtttgt atttattgta
    22501 ataactccag tagtaggatt tttttcaaaa gatatgagtt tagaagatag aatattaaaa
    22561 tctatgggaa actacaacaa gcagtatgta gatagtacaa atgctttggc aaaagagact
    22621 ggaaataata gtctatccaa gggatatgag gatgggttaa aagaagtact taaattaaaa
    22681 cttgatgaat atggttatga tttagaagat atagagctta atggggcaaa tataaataat
    22741 ataaaaataa aagaaaagaa taatagtgca aaaagtaata acagttccaa tgaagaaaat
    22801 aaaaataata gtactaaaag agaggaagaa aacataaact ctaatgacaa agaaaattct
    22861 aaacaagtct tcaaaaaagg tacagagtat gggttgaacc taaatgaaga aaaattaaaa
    22921 aacgacttaa taaaggtgct ggatgtttct atagaagata tacaaattga taaataggag
    22981 ttgggagaat gttgaaaaac ttaaatgaca aagataagag aaaggtatat tcactaataa
    23041 ctatagcagg aatatgtgtg gtttcattag tattattatc ttgttttcct agtagcaaaa
    23101 gtgataaaca agttggaaaa acagagacta ataaaaatgc tgaaaagcaa gtcactaaaa
    23161 aacaagaaaa agatgattta gagtcaaaat taacagcaat actatctaaa attgatggtg
    23221 caggagacgt tgatgtaatg gttacatttg aatcaagtga agaaatacaa cctgctttta
    23281 attcaaataa tacaacagaa acaacagagg aaaaagatgc acaaggtgga gaaagaacag
    23341 ttacgacttc tagtgaaaat aaaacaatga taacttcaaa ttcaagtgac cctgtagtta
    23401 taaaaactac agagccaaag ataaagggag tgatagtggt agcaagtgga gcaagtgacc
    23461 caagtgttaa agagacactt tatagtgcag tacaaacatc attacaagta gctggtcatc
    23521 aagtagaaat atattctaaa taaatacata taaccggggg gataaatgaa tgaagtttaa
    23581 ttataaggga agaggatttg taataataac tttaactgca atgttagtgg tagtaggaac
    23641 agtgaattat caactaagta agaaatcttt attagaaaca tcaaaagagt ttaaggcata
    23701 tgaagaagca cagttgcaaa aaaatactga tgatagtgat agtagtaaga cagaggattc
    23761 tagtaatgta gataaacaag gtggtaagga aagtgctgat attgatatag tcgatagcaa
    23821 agctagtaaa gtaaaagaaa aggcaactga gacaagtaag gaaataaaag ctcaattatc
    23881 atcagagaaa aatatgaaaa aggcatctta tattttagat atgaaaatga atagagaaaa
    23941 acaaagaaat gagttagttc aagatttaaa tgaaatgata aataatccat ctacaacaga
    24001 agaatctaga aaagaagctt ctaatatgaa attaaatata gttaaaattc aagaaaaaga
    24061 acttcaaata gaaaaccttt tgagtacaaa aggatatgaa gaagcgttag tttatataag
    24121 cgataacaaa gttaatgtag ttgtaaatga agctaaatta gaaaagaaag atgcagcaaa
    24181 aatatttgat ttagttgcag aacaagccaa tgtgaagtat gaaaatatta aacttacaaa
    24241 taataatagt aataagtaaa gtataaacct cagaattaac tgaggttatt ttcttgcaaa
    24301 gtagacttgt atttggtatt attatactta taaaaatagt tccttaaaat caggaggtaa
    24361 gttttaatat ggaagataat aaatttggac aagtaaaaat atctaatgat gtaatagcta
    24421 caatagctgg tttagcagca ttagaagttg aaggcataga aactacagca acattaacag
    24481 ataagttgtt aaaaaataat ggagtaaaaa tacaaatcga agaagaggat gttaatttag
    24541 atgtaatggt tacgataaag tatggaatgt caataccaga tacagctttt aaagttcaag
    24601 aaaatgtaaa aaatactgtt gagacaatga caggactgaa ggtttctcaa gtaaatatac
    24661 acattcaagg aatcagcttt aagaaggata aagttgataa agaagaagca aaagcaacta
    24721 agaaaaatta atattagccc tctgaaaagt cagagggttt tttgctggta attgatttta
    24781 tatggataat taaacttaaa ggagaatttt gattgatgaa aaaagatagg gcacaaaaga
    24841 gtacaacaag agaatatata atgaaactta tataccaaat aaatattaat aaagaagatt
    24901 ttgaaacttt agaagataaa gtagataatt ttttaaaaga taattcagaa catattataa
    24961 atagatataa agaacttgca cttcaatact caaaaaatac aaatttaaag ttagaagata
    25021 ctgaaattga agatgttata gacaagaaat atataaatac agtatgcaaa gcattaaaag
    25081 aaaatcatga caaaatagat gagttaataa ataagcatgc taaaaactgg acagtagata
    25141 gaatgccaaa ggtagatgtt tctatactta gattgtctgt gtgtgaaata ctttatttag
    25201 acactcctaa taaagtatca ataaatgaag cggtagaact tgcaaaaata tattgtgatg
    25261 ataagtctcc taaatttata aatggtatat tgggaagtgt tgttgatgaa attgggaaat
    25321 aatataataa ttggaattga tactagctgc tatactactt ctatagcagc tatctcttta
    25381 gataagaaag ttatattcaa tgaaaaaata atgttagaag taagggataa ttcaaaaggt
    25441 ttaaggcaaa gtgaagctgt atttcaacat atcaataatt tagggattct tagtgatagg
    25501 ataaaatctt ttaaagataa gtttaatgta gaaggagttt gctcatcaaa aaaaccgaga
    25561 cctgttgaaa actcatatat gcctgtattc aatgtaggtc ataattttgg aaaattattg
    25621 tctagtatat atggttgtag gttttatgag actactcatc aagagaatca tattgaagca
    25681 agtttattaa atagtaaatt aaaaaataat aataaattta tttctgttca tatgtctggt
    25741 ggtactacag agatactttt aacaagcaaa caagatagtc atcacaatgt atgtgatact
    25801 aatttaggta aaattgccaa aataagtatt aaaaaagatg ataaaagtaa gttatacaat
    25861 aattttggat ataatataga tataattgga ggaagtaaag atattagctt tggtcaactc
    25921 atagatagag taggaattaa gcttggatat aaatttcctt caggaaagta tttggatgaa
    25981 aatgctttaa attgtaatct taaaatagaa agtggtctga aaacttctgt aagagatggg
    26041 tatatgaatt tatctggatt agaaaatcag gtaaataaga ttataaatga taatggagat
    26101 aatactaatc aaaaagaata catatctaaa ttagtattag attcagtagt tagaaatatg
    26161 tttaaatctt tagtatattt atgtgaaact tataatgtaa atgaagtagt atttgctggt
    26221 ggagtttctg caagtaagta tattcttaga gaattaagca tgaagttaag aaaaaagcat
    26281 atagaagctt attttacaga accacaatat tcaactgata atgctgtcgg gtgtgctata
    26341 ataggattga ataacttttt aggagaaaga gtatgaaact aagagcttta gatataagtg
    26401 aagcaaactc atatattaaa agaattttaa taaacgaccc aatactttct aatcttaaag
    26461 ttaaaggaga aatttctaat tttaaggttc atagtagtgg gaatgtatat ttatcactta
    26521 aagatgaaac atcaaagtta aactgtgtaa tttttaaaag taattttaat cgcaatttaa
    26581 aattagataa tggtgtaaaa attatagcta atggatatat atcagtctat gaaagagatg
    26641 gtgcgtatca attatacatt aatgaaattg aaatagaggg aataggaaat ttacacatag
    26701 agtttaatag attaaaggaa aaacttaata aagaaggatt atttgaccct aaatacaaga
    26761 tacctatacc taaaatgcca aactcaattg gcgttataac atctccaaca ggtgctgtta
    26821 taagggatat aataaatgta ataaaaagaa gatatccgaa agttaatata aagctttatc
    26881 cagtaatggt tcaaggagat aaatcagctg aagaaatctg tgaagcaatt agatttttca
    26941 atcatatgaa gaatgttgat acacttatag ttggtagagg tgggggttca atagaagaac
    27001 tttggtcttt taatgatgaa atggtagcaa gagaggtttt taattctcaa attccgataa
    27061 tatcagcagt tgggcatgaa acggatttta ctatttgtga ctttgtgtct gatatgagag
    27121 ccccaacgcc atcagcagca gctgagattg ctactccatc attggatgat ataaactata
    27181 aacttggaaa cataaaaagt aggatgagta agtctcttac aaatcaaata gagctagacc
    27241 aatataggtt agaaacagta tttaataaga taaataacta tttagattcg tacacaataa
    27301 aagataaagt tatacaatta gacaaaatat atgataaaat aatttttgga atagaaaata
    27361 atttaaaatt agaagatgaa aaattagtaa aaattggagc attactccat aatttaagtc
    27421 cattagctac tatggataga ggttatagta taactcaaaa aaatggaaaa gtaataaata
    27481 gtataaaagg attaaaaata aaggatagta tagatatagt attaaaagat ggaaatttag
    27541 aatgtatgat agataaaatt gaaaataaag agggatagta tgaatttaac ctacgaagaa
    27601 gcttataaaa gattagaaag tattttaagt gaattagaat ctaaaaatgc tagcttggat
    27661 gaatctttaa gcctatatga agaaggtata agcctttata aacattgtaa taagctttta
    27721 gatgatgcta aattgaaaat aagtaaattt aatcaattag gaatagaaga agactttaaa
    27781 atagaggagg aataaaattg gaatttaaac aatgtctaaa agaaaaagca agttttgtag
    27841 aaaaagtact taaagaatat atgcctaaag aagaagggta tcaaaaaact gttattgaag
    27901 ctatgaacta tagtttaagt gctggaggta aaagattaag accaatactt acattggagg
    27961 cttgtaaaat agttggagga aatgaagatg aagctattcc ttttgctatt gcaattgaaa
    28021 tgatacatac atattcacta atacatgatg atttgccagc tcttgacaat gatgatttaa
    28081 gaagaggaag accaacaaat cataaagtat atggagaagc tatggggatt ttagcaggag
    28141 atgctttgtt aaactatgct tttgaagtaa tgcttgcagg ctcaatcaat aaagaaaatc
    28201 cagaaaagta tctaaaagct ataaatgaaa ttgccaaagg tgctggtata tatggaatga
    28261 taggtggtca agtagtagat gtagaaagcg aaaataaaca aattgaaaaa gaaaaattag
    28321 attatataca tatgaataaa actgctgcaa tgatggtagg ctgtatgaga gcaggagcta
    28381 ctataggtgg agcaaattct gaacaaatgg aagaaataac taaatatgca aaaaatatcg
    28441 gattatcatt ccagatagta gatgatatac tagacatagt aggagatgaa gctaagttag
    28501 gtaaaaaggt tggaagtgat atagaaaatc ataagtcaac atacccatct cttttaggtt
    28561 tagataaatc taaagaaatt gcacataatt tgattgatga agcaaagaaa agtatagaaa
    28621 aattatctga tgatgtagat tttttaaagg gattggctga atatataata gatagagaat
    28681 actaaaatat acagctaaaa tcgaggaggg aaaaatggat tttttttcgg agatttttaa
    28741 caatggggct ttgggaataa gtctgattgc ttgtttctta gcacaattta taaaaatatt
    28801 tactggtaag gaaaaaagaa ttgagttatc aagaatactt atttcaggag gtatgcctag
    28861 ttcccatagt tcttttgtta caagtttagc tacagtggta ggtattgaaa aagggtttaa
    28921 ttctactgat tttgctataa ttaccgtact tgccttaatt ataatgtatg atgcagcagg
    28981 tgttagacga gctgttggta aacaagctac tatattaaat caaatggtgg cagatataca
    29041 gcatggaaag catatagagc agaagaaatt aaaagaatta ataggtcata caccactaga
    29101 agtttggttt ggagctttac ttggaatagt aacagcttta atattgatgt aaattgaaaa
    29161 tttacagaat aaaggtgagg ataatatgta taaatattta gataaagtaa attctccaaa
    29221 ggatataaaa aatatgagta tagaagaaat ggatttactt gcaaaggata taagaaagtt
    29281 cttagtaaaa tctgtttcaa agacaggagg acacttagct tctaatctag gagtggttga
    29341 gttaacatta gcacttcata aagtatttga tagccctaaa gataagattg tatgggatgt
    29401 agggcatcaa tcttatgttc ataaaatagt tactggaaga aaagattgtt ttgtatcttt
    29461 gagacagttt aatggactaa gtggttttcc aaaggagaat gaaagtcctc atgatatttt
    29521 tgatacagga catagcagta catctatttc tatagctaca ggtattgcct gcgctaggga
    29581 tataaaaaaa gaaaactata gtgttatatc tgtgatagga gatggttcta taacaggagg
    29641 tatggcactt gaagctttaa atcaattagg atatatagat accaatatga tagtaatcct
    29701 taatgataat gagatgtcca tagataaaaa tgttggagga atgtctaaat atctgtcaag
    29761 tattataaga aactcaacag ttgagaaaat gacagatgaa gttgataaaa ttttaaatgt
    29821 tacacaaaca ggtgaaattt tatctaagac tgcacataga tttaaagata aactaatgta
    29881 tagcttttct cctcaggact gttcattttt tgattcatta ggtataagat attatggacc
    29941 tatagatggc cataatacta aagaactaat agatatattg agaaaagcta aacacaaaaa
    30001 agggcctgtt cttttacatg taattacgaa aaaaggaaaa gggtacagat ttgcagaaga
    30061 acaacctgat aaatatcatg gagtatcaaa gtttgatata aagacaggtg ttacatctgc
    30121 aaaagttaag tctatgtcaa ttagtgttgg agaaaaactt gttgatatgg ctaataataa
    30181 tgaagatata gtagcaataa cagctgcaat gccatctggt acagggttaa acctgtttga
    30241 aagtgcatat ccaaaaagat attatgatgt tggtatagct gaacaacatg ccacaggatt
    30301 tgcagcaggt cttgcaaaaa atggtatgaa accttacttt gctgtgtact catcattttt
    30361 gcaaagggca tatgaccaag ttattcatga tgtatgcata acaaaaaagc cagttacttt
    30421 tcttatagat agagctggat tagttggaaa tgatggagaa actcatcatg gtatgtttga
    30481 tttaagttac ttaaattcta ttccaaatat tgtagtaatg gctccaaaag acacaagaga
    30541 gatggagctt atgatggatt tgtcattaaa attagattgt ccactagcaa ttagataccc
    30601 aagaggaagt agttattacc tagataaggg agaatatgga gagattgtac tgggtaaata
    30661 tgaggtatta gatgacggtc aagatacagt catactttgt attggaagta tggtaaaaca
    30721 tgctttagag gctaaagaaa tattatcaag agaaggaata aatccgacaa tagttaatgc
    30781 tagattctta aagccaattg atgaaggtat gttaaaagca ttattaaaaa atcataaaaa
    30841 tgtagttact atagaggata atattgtaac tggaggattt ggaagtagaa taaacaagtt
    30901 tattatagat aatgaatata acgtaaatat acttaatata gcaataccag aagaatttgt
    30961 aaaacatggc aatatagatg aattatatga ttttgtagga ttatcgccaa aaagtattgc
    31021 tgataaaata aggaaattag ttattgagta aaaatctaat gtagatattg taaattaaaa
    31081 tttatataaa atatagaatt tatcaattag aataaatatc aagtgtgtta cttcaagcta
    31141 ctttagttat cattaaagta gcttttaaca gaaaggtaat tgtatgaaaa aaagaataga
    31201 tttattatta gtagaacaag ggtactttga aagtagagaa agagccaaaa aagcaataat
    31261 ggctggattg gtttttgttg ataatcaaag gtgtgataag gctggtacag aagtaaaaga
    31321 agattgtagt atagaagtaa aaggaaatcc aataccatat gtcagtagag gtggtttaaa
    31381 gcttgaaaaa gcaatgaaaa actttgattt gactattgat ggaaaggtat gtatggatat
    31441 aggggcatcc acaggtggtt ttacagattg tatgctcaaa aatggagcaa taaaagtatt
    31501 ttctatagat gttggttatg gtcaacttgc atggaaattg agacaagatg atagggttgt
    31561 gtgtatggaa agaactaata taagaaatgt tacaatagaa tatacaaagc agtttgctga
    31621 ttttgcgtca attgatgtgt cttttatatc gttaaagtta gtacttccaa aggcaaagga
    31681 gttagtacgt catgatgggg aagttgtcgc tttaattaaa cctcaatttg aagcaggaag
    31741 agaaaaagta ggtaaaaaag gagtagttag agaaaaatct acacatatag aagttataaa
    31801 aatgatttct gatttctcag tcgaaaatgg atttgagatt ttagggttag atttttctcc
    31861 tattaaaggt cctgaaggta atatagaata tctaattcat ttgagaaatg gaaatgaagg
    31921 atatgaattt gatggtgaga catacaataa caaaatagta gaagttgttg aagcttctca
    31981 taatttagac aaataggtta aaagggggct tagatttgat cctcgaatta tatatgaaaa
    32041 attgtgcctt agttgaagaa ctaaggttga atattgataa aaacttaaat atacttacag
    32101 gtgaaactgg ttcaggaaaa tctataataa tagatgcact tggactttgt ttaggtgata
    32161 aatatgatag gtctttttta agaaaaggaa cagataaggg acttgttgag gctgtatttt
    32221 tttcagacaa tagatatctt aaaaaaatat tagaagaaaa tgatataagt atggaagatg
    32281 ataatttatt ggtaataact aggttgatat actcagatgg taagagtact gctagagtaa
    32341 atggaaggac tgttaaagtg tctcttctta aagaaattgc atctacactt atagatatac
    32401 atggtcaaca tcaaaatcaa gctttgttca ataaagatac tcatttaaaa tttctagacc
    32461 tttttggaga aaatgaattg gaagagttta aaattgctta taaaaatgtg taccataagt
    32521 atagtgaagt aaaaaaagct ttaaattgct tgacagaaaa taaagatgag atgcaaattc
    32581 aacgagaaat agatttatta agatttcaaa taaatgaaat tgaagctgca aatctaaata
    32641 agaatgaata tgaagattta ttaaaacaaa gagaagttta tagaaatagt gaaaagatat
    32701 ataataatct aaattcgagt tatagcaagt tacataatgg tgagtataat gttattgacc
    32761 taataggtct tgcatctaag gaacttaatg acatatctaa atatgacagt gttttaagtg
    32821 aatatagtga tactgtagaa agaattatgt atgagttaca agacatatct ggtgagataa
    32881 gaaattacaa agataatata gactttgaac cttatgagct agagcagatt gagcttagaa
    32941 tcgacgaaat taataattta agaagaaaat atggagacag tatagatgat atatttgagt
    33001 attatggaaa aattaaagat agattagatg aaatattaaa tagagatgaa agagttgaac
    33061 agttaaaaag caaactgatg aatatagaag aagatttaaa aataaaagcc agtaaattga
    33121 ctaaagcaag aatagaagta gcaactactt tagaaaaaat acttcttgat gaattaaaaa
    33181 gcttaaatat gaaaaatgta atgttcaagg ttaattttga aaaaggttca tttactttag
    33241 atggagtaga tgatattgaa tttatgatat cttttaactt aggagaagat attaagccta
    33301 tatataaagt tgcatcaggt ggtgagatgt ccagatttat gttagcgttt aaaaccatat
    33361 tggctgatat agatgatatt gatacattag tgtttgatga gatagatact ggaataagtg
    33421 ggatagcagc acagatagtt ggtgagaaat tgagtgatat agcaaagaaa aaacagataa
    33481 tatgtataac tcatttacct caaattgctg caaatgcaga cactcattat tgtatagaga
    33541 aagatacttc aaataatagg acgtttacca atgttagtaa gttaaatgaa tctcaacgaa
    33601 aaaatgaaat tgccagactt atagcaggta acaatataac agaaaaaact attgaacatg
    33661 caagtgaaat cattgaattg gctaaaaaat gttagtgaaa aacaagcatt ttaataggtg
    33721 taaagaattt ttatagaaat gctttacacc tattttttat gatgaaatta ttttaaaata
    33781 tctagtgcct atattgaaga aaagtaggtt tgaaaatgga atgaactttg tataaatttg
    33841 taactttgaa atttctaaat gcttaagtat aatattattt attaaggtag tagtgagggg
    33901 aggcataaaa tgaataaaaa agatactatg cacttaattt ttataattgt aatttttatt
    33961 ttattatggt atattgtggt agcagtaagt gagattaata aaaatattga aagttgtagt
    34021 aacaaattag attatttgtc acaagagctt atgagtacta agtcggatat tagtaatact
    34081 ataaatgaag aaatgtcaaa aagttatata acaaaaagta ttgacttaaa agtaaaaaaa
    34141 atcgagaaaa aagaatgtac tattgatgta gatgttcaat taagtaaact tggaaaaaat
    34201 ggaaaagtat tttttatgta taaagaagat agtgataaat ggaatgaaac tgagatgaca
    34261 aaacatgggg aattatcata tgcatgtgaa ataaagataa atacaggaag tgaatatgat
    34321 tataaagtag ttacaagtgg agatatatct gaaagttctg atgtacaaaa actgactaaa
    34381 tctgattata tgcctgaaca acctatattt aattctggga taagagataa tagagaatac
    34441 tttattgaaa tagtagaaaa tagtaatctt tttaatcatg agagtgaaaa gtcaaaaaat
    34501 aaagcttacg ataatatcag gttagaaaaa gtagatatta ttgtaaatga aggaaaaaaa
    34561 gacactatat acaaagccca attagctgaa ggcttagatg atggcaaaac taataattca
    34621 aatagaaatc aagtgaatta tgaagtaagt tttcctaaaa gggatattga tgacataatt
    34681 tatattaaag ctaaacttac atacaataat ggagtagaat atacaaagga tataactgaa
    34741 gatataacta tgggattaca agatttagaa aaataaatac atatttaaat aaatattcaa
    34801 atagatttgt tcacaaatag agtataaata aaactgtagt agttttactt atactctatt
    34861 aaaaaattat aattttaatt ccatatatct agccccttca attggattat aagtataaag
    34921 ttctatatca taaaaaccaa acttttcata taatccttgt gcacttttca tgcttggaag
    34981 agtatctaat ctcatgtgtg tatatccaat ttttttagct tctacaatga tttcttccaa
    35041 tagtattttt cctattttta atcctctaaa ttgatttctt acatataatc tttttaattc
    35101 acatatttta ccttcaagtt tttttaaagc aacacaacct gccaaatttt catcaacaaa
    35161 tgccaatatc aatgagcctg aaggtttttt atattttcct ggaagtgttt ttaattcatt
    35221 attaaagtct tgaaaacata agtctatatt tagagaattt gaatattcag taaatagaag
    35281 ttttacgttt tcaagatttt ctaaatcttt ttcttcaaca aattgtaaag ttatattttg
    35341 agccatttta tccgccatta tataaacatg tttatataat gacttcacct ccttgatata
    35401 tgtatatttt tcttaatatc aattagattt taataccatt actttttcac caccatagtc
    35461 aagctctcca ttaaacttaa atccaaaatc tttataaagc ttaatagcca ttttattgtc
    35521 ttcgaatata ctaagatata gttcatcaca gttatattca tttttaagat gatttacaag
    35581 aaaatctaat gatgcttttc cacatccttt accttgatat tttgaatcaa tcataaatct
    35641 atcaagccaa actctatcat cttctaaacg accatacata gcaaatccta ttaaagaatt
    35701 ttcatcatat attccaacag gtacccaagc tgttataaat ttagattcag caatagaaag
    35761 tgcattgctt tctatgtaat ctaactgact tttatctaca gacaaactaa ctaaatcgtg
    35821 ccaattattt atgtctgcaa ttcttacatt taatttcaag atattaccgt ccttcaaact
    35881 ttttgttaca atctaatact atcatattat gtattaaaaa tcacttatta agattttaaa
    35941 taaacttttt gaatgaataa attctcagga aaaaggaatt ataaatacat aataatacag
    36001 cataggagta gatggatata tgaacaaaat aaataaaaat ttcaaaaata tttggataat
    36061 attaggagct gtatcgattt ttaccatggt tataattttt aataaaaatt gtaatgttaa
    36121 taaagagact caaatagaag cttttacaat taagaatcat gataaaaaat ttgtatatcc
    36181 tatgggaaat ataattggag taaaagcaaa tacagatggt gtattggtct taggatatga
    36241 ggaagaggat gtaggctata ttggaggcat acaaataggt gataatatag taaaaataga
    36301 taataaaagg ataaaaaata gtcaagatgt atcagagatt ttgaataaga ttaaaaaaag
    36361 taaagttgaa gtgacttttg agagaaaaaa taagtataaa acagaagtaa tagagactaa
    36421 aaaagaaaat ggcaagtata aacttggatt atgggtaaga gataaaatat ctggaattgg
    36481 aactatgact ttttacgacc ctagtatgga aaagttcaaa gcaatcggtc atgccataaa
    36541 agattcggat actaatgaac tattaaagat aaagcaaggc tatatatata agccagaaca
    36601 attaaaaata gtaaaagcta gtaatgaaaa agtaggtaaa ataaaaggag attttaatga
    36661 cagcaattta atgggtaatt tctcaaataa ttcagagcta ggtataagtg gtaatattac
    36721 agaaaaccat aataaagagt ttaatgtagc aaataaagaa aagcaattaa tagaagttgg
    36781 aagaccgcaa gatgtaaaaa taggagatgc agtaattctt tttgaggata aaaacaaaaa
    36841 tataacaagc tatgatataa aaatagaaag tatagtatat gataaaggaa attatagaga
    36901 tatggtaata aaagtagtag atgacaagtt attggaatac acaggaggta tcgtacaggg
    36961 gatgagtgga gctccaataa tacaaaataa taaaattatt ggtgcaataa ctcatgtttt
    37021 tagagataat ccgaaaaaag gatatggtat ttttatagat gaaatgataa aattgtaggt
    37081 gaggcattaa aaaattttat tattttatca attatctagg aggaatataa ttttggagtg
    37141 tcgaatatgc tttagagtag ataattagga agcaattgtg taaaaagttt agttttctgt
    37201 aataagaaga tgttttttaa tggggggatt tttagtggaa aaaatcaaaa tagttttagc
    37261 agatgacaat aaggattttt gtcaggtatt aaaagagtat ttgtctaatg aagatgatat
    37321 cgatatatta ggcatagcta aggatggaat tgaagcatta gacttagtaa aaaagacaca
    37381 accggactta ttaatactag atgtaataat gccacatcta gatggattag gtgtaattga
    37441 aaaattgaat actatggata tacctaaaat gccaaaaata atagtactat cagcagtagg
    37501 tcaagataag ataactcaaa gcgcaataaa tctaggagca gattactaca tagtaaagcc
    37561 atttgatttt gttgtgttta taaacagaat tagagagcta gtttctaata gagttacaca
    37621 agttgagcca aaacctagac cagttcaaga gactcaaatg actagaagtg attttgttaa
    37681 gaatgtagga aatatagaga cagaaatcac aaatataata catgaaatag gagtaccagc
    37741 tcatataaaa ggatatttat atttaagaga agcaataaaa atggttattg ataatgttga
    37801 gcttttaggt gcagtaacaa aagagttata tccaagtata gctaaaaaat tcaatacaac
    37861 accaagtaga gttgaaagag caataagaca tgcaatagag gttgcatgga gtagaggaaa
    37921 agttgacaca ataaatcaat tatttggata tacggtacac aatactaaag gaaaaccaac
    37981 taattcagaa tttatagcaa tgattgctga taaattaaga ctagaacata gtatggttaa
    38041 ataaacaaga cataaaaagt aaggcttttt aattaaggca ttggctataa atgcgtatta
    38101 caagcagcga aacggtataa ccactagggt tataccgttt cgctatttta ataaatataa
    38161 aaattttctt tattatttgc ttactatatc aatataataa ttttattata ctatggatat
    38221 agtatgtgtc tttacaagtt gtaaactgac agtggtttat tttttaatat aaatattgac
    38281 tttgatgcag gtaaactttg tatttttaag cgtattatgg aatatgttaa ataaaaaaat
    38341 gatgaaatat agtattgtaa atgccaaaga tgcaaaacaa acttaaaaca tttattttat
    38401 tgttaagtaa tgctataata taatgtgatt ttaataatga tagtggaggt ttaaatatga
    38461 gagtcgaggc ccctataaaa gtagatcgaa aaaccaaaaa acttgctaaa agagttgaaa
    38521 gtggggaaat agcagttata aatcatatag acatagatga agttgctgca aactctttag
    38581 tagaagctaa aataaaactt gtcataaatg cggctccttc tataagtggt aggtatccca
    38641 ataaaggtcc agggatatta actgaaaata atatactaat aattgataat gttggagaag
    38701 agttatttga agaattaaaa gaaggagaaa ctatagaagt tgtagatgga aagatatata
    38761 gaaaaggaaa atttttaggt gcaggagaag ttttagacaa gtatgaggtt tcacaccaaa
    38821 taaaggctgc ctatgagaat ttagctgtag aattggatag atttatagat aatactatag
    38881 attatgctaa aaaagaaaaa ggatttatac ttggagaagt agaaattcca aaagtgaaaa
    38941 ctaattatgc taataaacat gtattgatag ttgtaagagg tcaggactac aaagaagatt
    39001 taagtactat gctttcttat atagaagagg taaagccagt attggttggt gttgatggag
    39061 gagcagacgc tttaattgag tttggatata ctccagatgt gattgtaggt gatatggata
    39121 gtgtaagtga tgaagcttta aaaaaggcta gtgaaatagt agtacatgct tatacagatg
    39181 gaagagcacc aggtcttaag agggttgagg aattaggctt agatgcagtt gtatttcctg
    39241 cgccaggaac tagtgaagat atagctatgc ttatcgccta tgagtataag gctgagttaa
    39301 tagtagcagt tggtacacat tcaaatatga ttgattttct tgaaaaagga agaaaaggta
    39361 tggcaagtac ttttttagtt agactaaaaa taggttcaaa gcttatagat gctaaaggcg
    39421 ttaatttgtt atacagaagt aaattaaaaa taaagtacat atgggcatta atagcaacag
    39481 cattatttcc tgtgttggta gttgcatcat tgtcaccagg agttcaacag ttcatacaac
    39541 taatgcaatt aaagtttaga gtattattac agatgtaaat tttaggagga taaatatgca
    39601 tattaatatg aaatattata tagtcactat aggggctata tttatagctt tgggtatagg
    39661 catgttagta ggatttaatt taaataataa tcaacaatta agtgaacaac aagcaaacat
    39721 aataaatgat ttggatgata agtttaatat cttaaaagaa aaaaatgata aacttgacag
    39781 tgacttagca agtgtaaata gagattatga agaggcagtt aattttataa ataaaaatgt
    39841 agataagatt ttagctggtt cgctgaatgg taaaagtata ggaataatat cggctaatga
    39901 aaatgacgat tatacaaaaa atatagaaga cataataaat aaatctaatg gaagtattgc
    39961 atttaatata gtattaaaag aaaatataac aaatccagaa aagttaaagg aaatgtcaac
    40021 taaactaggt acagatgtaa aaaatgcaaa tgatgcagtt aattatataa ttgacaccct
    40081 taaaaaggaa gatgcaagtg acattttaac atatcttcaa gaactagatg taataaagtt
    40141 taactctctt ggaaatacat atctaaaata tgattctgtt gtaatagctg gtggaaatga
    40201 tgcaaaagac agtacaaaac agtttgaaaa aatagaaaaa tttgtagttt caaaacttaa
    40261 atcagagaat aaatatttag tagaagttca aaatacagga gttaaaactt cttatgtaga
    40321 actatattct aaaaacaaag ttgcaactat agacaatatt gatgaaggta taggaagtat
    40381 atctctagct attcttctac aacaaggtaa tatagttggt aattttggta gattggatac
    40441 tgcaacttca ttgttgccat ctattaaata gaaataattt taaggagaga aaaaatggat
    40501 tcttatataa gtataataat accagcttat aatgaagaaa gtagaataaa atatacatta
    40561 gaaagcatat tggacattaa gcaaataaat gaaataatag ttgtggatga tggttcaatt
    40621 gacaatactg ctaaggttgt atctgagcta gaaaatgaaa agattaaatt ttttaagtta
    40681 gataaaaata gaggtaaagg atatgcttta aattatggta tcaaattagc aatgaaaaat
    40741 gcagatatta taggttttct ggatggagat ttaggaagct ctgctaaaga agttgaaaaa
    40801 ttaatagtac ctatattaaa tggtgaggcg gatgttacaa tagccaagtt tcctcctgca
    40861 aagagaaaag gaggtttggg atttgtaaaa ggtttagcta aaaattctgt gtttgagatg
    40921 actggtgttg agttagatgc aactttatca ggtcaaagaa tatttaaaag agaagtttta
    40981 gaaaagtttg atgaaatacc ttttggatat ggagtagaag ttggaatgac cattgatata
    41041 ttaaagtatg gatttaaaat taaagaagta ttagtaaata tgactcataa tgaaacagga
    41101 agaaatttaa aaggttttat tcatagaggg aaacagtatt atcacataaa aaaggtttta
    41161 ggacaaaaaa agaaagaatg gaggtaatat cttagtgaaa gattatatac tttataccat
    41221 tttattatta actggattat ttggaaccta tgctgtaatt ccactattta aaaatttatt
    41281 gataaatgga aatgtactta gaccaaacta taaaaaagat atgataccag taagtatggg
    41341 aattgtattt ttgcctatga ttgtaatcaa tggaataata cttgctttct ttactacaga
    41401 atttaaggat ttagcgtata tatttatgtt tatgtttggt atgatatcca tgttttttgc
    41461 agggatacta gatgatgtca ttggaaatag agatgtaagt ggtttaaagg ggcattttaa
    41521 aagtttattt aaaggaagtc ttacaacagg aggttttaaa gctttatttg gtggatttgt
    41581 agggcttata gtttctgttg ctatatcaaa agacataatt gatataatag taaatacact
    41641 aataatagct ctatcaacta atttaatgaa tcttttggat ttaagaccag gaagggcaat
    41701 aaaagcatat ttattcataa tggtaattat atttttaaca ttgacaggat ttgttcaagt
    41761 tcttccatta cttatagtac caaatgtact tgcatacttt aattatgatt taaaagcaag
    41821 ggctatgatg ggagatactg gttcaaacgt ccttggaata tctattggaa tgctcatttc
    41881 ttttgggtat ccatttaaca ttagattagg atggttaata tttgtagtta ttatgcatat
    41941 attcactgag aaatactcat taactaagat aatagaaaaa aataaactat taaattttat
    42001 agataaattg ggaaggtagt attatgataa gcaaaagagt ggggattgtt gaatcaatag
    42061 taagtcaaac tgaaactcta gatgatataa gagtaaatat aaatggaaaa atacaaaggg
    42121 catataatta tcccaaaata tctggaacta taaatatagg tgatgaagtg gttttaaata
    42181 caacagcagt agaactgagc ttaggtacag gtggatacca ttttgtaata actaatttaa
    42241 ataatataga atctacttta actgaaggtg ggcatataat gaaacttaga tatacacctc
    42301 ttcaaataaa agtggattct gttgaggaac aagaaagtat ttatcatgat aaatttgcga
    42361 attttaaagg attagaaggt ctttctgttg tagtagggac tcttcatagt atgttaactc
    42421 catttgcagc ttcatttaaa agaaataatc caaataaaaa attagtatat ataatgactg
    42481 atggagcatc tcttccaatt tatttaagta aaaatgtgga cactttaaaa gaaaaaaaat
    42541 taatagatag tacgataact ataggaaatg cgtttggtgg agattatgaa tgtataaata
    42601 tatatactgc attaattaca gcaaaagaaa tactaaaagc ggatgcagta tttgtcagta
    42661 tgggacctgg aattgctggg acaggaacaa aatatggatt tacagggatt gaacaaggct
    42721 ctattcttga tgccgtaaaa aaacttgaag gaagagcaat tgcaattcca agaattagtt
    42781 ttgctgacaa acgagaaaga catcaaggta tttcacatca tagtatgact gtatttaaag
    42841 aaattgtaaa tgtaaatgta gacataccaa taactatata tgatgatgaa aaattgaact
    42901 ttataaaaga gcaaatcaat ttaaatggtt tagacaaaaa gcataatata atttatatag
    42961 ataataataa aacgaaagag gatttagact attttaatct aaaagtaaag agtatgggaa
    43021 gaaattatga acaagatgag gcatttttca aagctgcaag cacagcagca tattacttaa
    43081 tggaggttta agatggtatt agaagaaaaa actattagta gtgatagggt ttatacaggt
    43141 aaagtaatta ctttaaaggt tgatactgtt gaaattccag ggcaaggata tcaaaaaagg
    43201 gagttagtag aagttggtgg agcagtagga atagttgcaa tcacagatga taataaagtt
    43261 gtactagtta agcaatttag aaaacctata gaaaagccaa tctttgaaat tccagctggt
    43321 aaactagaaa aaaatgaaag tccaaaagaa tgtgctgaaa gagaattaaa agaagaaact
    43381 gggtatagtg caaaaaatat taagctaata cataaatttt tcacttcagc aggtttttct
    43441 aatgagataa tgtttgtata tttagcaaca ggtcttactc caggagagaa taatttagat
    43501 gcagatgagt ttttagatgt ctatgaaatt gagcttgaag aagcatataa catggtatta
    43561 aaaaatgacg ttgaagatgc aaaaacatct attggattat tattagtaaa agatatgttt
    43621 aaaaattaat atttataaaa tgaataaccc tttgtcctaa agcatattat tttataaagg
    43681 acaaggggga attaaattga gaagaacata tagaaaaaat gattttagag aattaaatag
    43741 acaaataatt attataggat tgttgtttat gatgtctatt gttgttgggt cttacataaa
    43801 taaaatattg ccaggttcta gtaacaatat attaaacaat ataaatcctg ctgtagagta
    43861 ttataatcta aatataagca taaaagatac agtgatacaa aatttaaaat cagatgcaat
    43921 ctttatgggc tcaatagctc tattaagtct atttgtagta acaattccag cagtgttggt
    43981 agcttttgta ttaaaaggga tgtcaattgg ttatacaata aatagttgta tattggcatt
    44041 aaaatttaaa agtataaaaa tgattttaat aatattattt aaaaatttga ttattattcc
    44101 atgtgcaatc atattagcat taattagttt aagttatttt aaagaaatgg tttatgaatt
    44161 taaaaagaaa aatagaaaaa atatgcaatt tttaattaag aggtatatac taaatattat
    44221 aattataata gcactatcat tggggttgca attaatatta aatactgtaa gtattggtat
    44281 tattaagttc ctagctaaat aaaggagttt ataatggata ttatagaggg atatatagat
    44341 tacataaaag ataaaaaaaa attatcagag aacacagtgg catcatactt tatggatata
    44401 aaaaaatata tggaatatat aaatcaaaaa agcattaaac tagtagatat agtagaaaat
    44461 gatattataa gttacttaat aaagttggaa aaagataatg tatcaatagc tacaatagcg
    44521 aggatgatat cctctataaa atcttttcat gattatttat ttttaaatca tatatgtaca
    44581 aataatcctg caaaggatat aaaaaaacct aaaataaaaa aagaaaatat aaatatactt
    44641 acagaagaag aaattgaaag actattaaat tttccaaagt taacaacacc taaattgatt
    44701 agggacaagg ctatttttga agtattatat ggtactggta taaaagtatc tgaattagta
    44761 gagatgaata tagaggatat tgacctagat attgattata tatattgtaa ttcatgttgt
    44821 aaaaatcaaa gagtgatacc actttgtgat attacaaaac tttacttaga aaagtatcta
    44881 aaagaagcaa gacctaaaat ggctgtagaa ggtgaaaaat cattattcgt aagttcatta
    44941 ggtcaaaggt ttacacgaca aggtctttgg aaagtcataa aaaaatattc taatctagcc
    45001 aatatagata aaaatataaa tcctacaatg cttagacatt catttgctat tcatttgtta
    45061 aatgaaggtg ctaacattgc tgttgtaagt aaaattttag gaaatgttaa tttatctagt
    45121 ttgcaagtat atttaaatca tatagataaa aacgtgagaa gagaaataaa ggaaaaacat
    45181 cctagaaatg atgttgatgt agaattacaa aaagcagaag ctaaatagac tatatagcaa
    45241 attttataat ttaaaaggag tatatttata tgagcagagt aatttggatt gtaattgata
    45301 gtgttgggat aggagcatta cctgatgctg aaaaatttgg tgatagtaag gatgtaagta
    45361 cccttggaaa tatatttaag gaatatccag atattcagat tcctaatatg agaaatttag
    45421 gtataggaaa catagatggt atagattttt ttgaaagtat aaaagaacca ataggttgtt
    45481 ttggaaagtg taaggaaatg tctcagggaa aagatactac tacaggtcat tgggaaatga
    45541 ctggtataat agttgacaaa ccatttaaga catttgaaca tggattctct aaggagataa
    45601 tagaagagtt tgagaaaaaa actggaagaa aagtagtagg aaataaacca gcttcaggaa
    45661 ctgtaataat agatgagtat ggagagcatc aaataaaaac tggagatgta atagtatata
    45721 catcagctga tagtgtattt cagattgctg ctaatgaaga agtaattccc ctagaagaac
    45781 tctataatat gtgcaaaatc gctagggaaa taatgatggg ggataatgca gtagctagag
    45841 taattgctag accattcata ggtaagaaaa aaggtgagtt tgtgaggact tcaaatagaa
    45901 gagattattc actagaccca tttgaaccaa cagttcttga taatataaaa gaatcaggac
    45961 ttgatgtttt agcagttgga aaaatagaag atatttttaa tggaaaagga attacagatg
    46021 ctatccacac taaaagcaat atggatggag tggatgaaac tttaaattat atgaaacaag
    46081 ataataaagg tctaatatat tcaaatctcg ttgattttga ttctaaatat ggtcatagaa
    46141 gagacccaga aggatataag aaagctcttg aagagtttga tagtagactt cctgaaataa
    46201 tggctaatat gagagaagat gatattttaa taatcaatgc agaccatgga aatgacccaa
    46261 catataaagg tacagaccat acaagagaat atatacctgt tatgatttat ggcaataaga
    46321 ttaaaaaagg ttttaattta ggagtaaaag atacttttgc tgatattgga gcaactgttg
    46381 cagatatact aaatgtaaag ttgcctaaac atgggtcaag ctttaaagaa gatttatttt
    46441 aaactataaa atgataaaaa cttagcttaa taggatggag agattggaat gtttgaaaag
    46501 atagcacaaa gtagtaaatt tataaattct aaaagtaata taaaacctaa gattggtttg
    46561 atattaggtt ctggactagg tgatttagca aatgatatag aagaccctgt cacaattaaa
    46621 tatagtgaaa ttccaaactt ccctgtatca acagtagcag gacatgctgg acaacttgta
    46681 attggaaaac ttgaaggaaa agaagttatt gctatgcaag gtagatttca ttactatgaa
    46741 ggatattctc agaaagaagc tacttttcca gtaagggtta tgaaagaatt aggagtcgag
    46801 attcttatag taactaacgc tgctggaggc gtaaataaag aatttaaagc aggagatttg
    46861 atgcttataa gagaccatat aaactttagt ggaagtaatc cattagtagg aaaaaatgat
    46921 gacagatttg gtgctagatt tccagatatg tcagatgcat attcttctaa atatgtagat
    46981 gtagttaaaa actgcgctaa agaatgtaat atagatgtca aagaaggcgt ttatatgttt
    47041 tttagtggtc caaactatga aacaccagca gaagtgagaa tggcacaaat tttgggagct
    47101 gatgctgtag gtatgtctac tgttccagaa gtcatagtag cttctcactc caatatagga
    47161 gttattggta tatcttgtat aacaaatatg gctgctggca tcttaaatca accactaagt
    47221 catgaggaag taatagaaac tactcaaaaa gttaaatcag aatttatgaa tttagttaaa
    47281 acaacggtta aaaatttata agttaaagct tattatatat tttttgagga agtttataat
    47341 aaaatagtta atgtttaatt tgaaaagaat taataaaaag ctgtcttgat atagaaagtt
    47401 atttctaata aaggacagtt tttttataat actacaacat ttgaaagtgt tttaaagttt
    47461 aatatttatt taaattatta tagattagaa tggagatttc aattatgaga atgtatgata
    47521 ttataaaaaa aaagagagat aacttagagc ttagtaaaaa agagataaac tactttatag
    47581 aaaactattc aaagggagat atacctgact atcaagcttc tgcactatta atgtcgattt
    47641 atttaaataa gatgaataaa caggaaactg tacatcttac agaagcaatg atgaactctg
    47701 gagacatgat aaacctgtct gaggtagatg gaataaaggt agataagcat agtactggtg
    47761 gtgttggaga taagactacc atagctctta tacctttggt tgcttcatgt ggagcaccag
    47821 ttgcaaagat gtcaggtaga ggtcttggtc atactggtgg tactattgat aaattggagt
    47881 ctataccagg tttttcgaca gaaatggaga taagtaaatt tattgattct gtaaataagt
    47941 ctaaaatagc agtttgtggt caaagtgcaa aggtagcaat tgcagataag aaaatatatg
    48001 ctcttagaga tgttacagca actgttgata atatatcatt gatatcatca agtgtaatgt
    48061 ctaaaaagtt agcttcagga gctgatgcaa ttgtgttaga tgtaaaaact ggtgatggag
    48121 catttatgaa gacattagaa gagtcttttg agttagctaa atctatggtt gatataggta
    48181 ctggtatgaa tagagataca atagggattg taacagatat ggatgaacct ttaggttttg
    48241 cagtgggaaa ctctttagaa gttatagaag caattgagac attaaaagga aatggcccta
    48301 aagaccttgt tgagctatgt gaagtattag gagcatatat gttggtttta tcaaaggtag
    48361 cacatgattt tgaagatggt gttgaaaaga taagagaatc aataaaatct ggtactgcta
    48421 tacaaaaact caaagagttt atagagaatc aaggtggtaa taaagatgta gtagatgatt
    48481 attctttatt caaaaaatca aagtataaat atccagttaa atcaattaaa tcaggttata
    48541 taagtaaaat taaagctgaa gatgttggtg tatcagctat gattcttgga gcaggtagag
    48601 aaactaaaga tgatatttta gatttatcag cagggataat tttagaaaag aaagtaggag
    48661 attatgtaaa tgaaggtgaa gttttagctt acatgtatta taatgatgaa caaaagcttt
    48721 cattggcaaa gggaagattt attgattcct actctattgt agattcaaaa atagaaaaaa
    48781 ataaattaat atatggtatt gtaactaaag aagagattaa aaagttttag aaaattttag
    48841 aaaagtgatt ataaaaggcc taacatgtca aaaataatgt taggtttttt attttttgat
    48901 aaactataaa ttataccaaa aatatatatt ttaaaacatt ttttacaata taaataagtt
    48961 tattctacaa gatgattgaa aattaaagta tctagtttta taatagaaaa tggataaata
    49021 tacataagga gtagattata ttatgaactt taaagaaaat agattgaaaa tagcagtatt
    49081 aataattgtt attttgatta tcttagcagg tatatttgtg tttatacaaa taggtcctta
    49141 tgataaaaat aacaaaaaag atgtaattat tgacgttcca agtggagcat ctgttggaaa
    49201 aatatctgac atattatatg aaaataaatt aataaagaat gaactattat ttaaactttt
    49261 ggtaaaggtt agcaataaag ctccgtctat aaaatcagga acatacttac ttaatcaatc
    49321 gtattcaaac aatgatataa taagtctttt agtttcaggt aaaatatatc aggatggtat
    49381 aaaagtgact attccagagg gagctacatc taaagaaatt atagcaatgt tagtaagtaa
    49441 aaatttagga gataaagcta cttttgaaaa tttaataaaa aaacctcaag aattttatga
    49501 taaatttcca tatttgaagg aagatggaat aacctcttta gaagggttct tgtatccaga
    49561 aacttattat tttaattcaa agaaacaatc agaagaagat attttaagcg aaatgttaaa
    49621 agtatttgac agtaaataca cggacaagtt taaaaagaaa caaaaagaat taaatatgac
    49681 tcttcaagaa gtaatggaaa tggcatcaat aattgagaaa gaagctgttc ttgacaaaga
    49741 cagacctata atagcaagtg tgttttataa tagacttaag gtaggtatgc ctcttcaatc
    49801 agatgctaca atacaatata tatttgaaga gagaaagaaa attgtaacat atgatgattt
    49861 aaaaattgat tcaccatata atagttataa aaataaggga ttacctccta caccaatatc
    49921 aaatcctggt ataaaatcta ttgaagcagc attatatcca gagaaaacag actatttata
    49981 ttttgtagct aagattgatg gtggaaataa ttatagtaca aactatcaag accatttaaa
    50041 atacgttaaa gaatataaag aagcaaggga taaacaatca aaagatacaa aagcaacaaa
    50101 taaagaaaat acaaaaaaat aaaccatgat tataaaaagt ctaacagggt aagaataatg
    50161 ttgggctttt ttattgaaaa ttgttattct tagatttata ataaagaaag tgtaaatata
    50221 tataacaata tgaatttaaa aaataataaa ttaatataaa acttgaatat ttattatttt
    50281 agggagtgtt tttatgagta atatagttaa tgatttggtt gaagattata taagagtgac
    50341 attaaaagaa aaagaaggat ttttaaaaga tttagaaatt tatgcagagg aaaatagtgt
    50401 tcctataata cataaagaag tttcagattt attaaaagtc ttgttaaagg ttcaaaaacc
    50461 aaagagaatt ttagaagtag gatgtgcaat tggttattct tctatttttt ttgcaagtat
    50521 tataggaaaa gatgcagata taataactgt tgagagaaat gaaaaaatga tagaaaaagc
    50581 aaaagaaaac ataaaattag ctggatttga caataacata actatactag aaggagatgc
    50641 agaagaaaaa cttagtcaag tccaaggtga atttgacctt atatttatag atgcagccaa
    50701 aggtcaatat aagctatttt ttgatttagt tatagataaa ttaaaagatg ggggactttt
    50761 agtatctgat aatattttgt ataaaggtat ggttgctcat gatgattttg ttgtaagaag
    50821 aaaaaagact atagttaaaa gaatgagaaa ttatttagat tatatatgta attgtgacta
    50881 tttaagcaca tcattggtac caataggtga tggtgtagca ctaagttata aagaaagtaa
    50941 aagaggtgat gtagatggaa ttaaataaag tagaactttt agctccagca ggtgatttgg
    51001 agagattgaa aatagctata acatatggag cagatgccgt ttatataggt ggagaaattt
    51061 ttggaatgag gtctgcagcg aagaatttta gtaaggaaga tatggcagag ggagtagctt
    51121 ttgcacatga aagaggcaag aaagtattcg taacagtaaa tataatacct cataatgaag
    51181 attttctaca attagaagac tatttgttgg aactagaaga gataggaata gatgcagtta
    51241 taatagctga cccaggagtg cttagtgtca ttaaaaaagt aattccgaat atggaaatac
    51301 atttaagtac acaagcaaat acaaccaatt atctaagtgc aaacttctgg tacgagcatg
    51361 gaataaaaag agttgtagtg gcaagagaat tatcttttga tgaaatatct gagataagag
    51421 caaaaacacc tttagatatg gatatagaag cgtttatgca tggtgctatg tgtatttctt
    51481 attctggtag atgtttaata agcaactata tgactggaag agatgcaaac aaaggttctt
    51541 gtgctcaatc ttgtagatgg caatatcatt tggtggaaga aaaaagacct ggtgagtatt
    51601 ttccaatata cgaagatgaa aggggtacat tcttctttaa ctcaaaagat ttatgtatga
    51661 tagagtatat accagagtta atcaaatctg gtataacaag tttaaaaata gaaggtagaa
    51721 tgaagactgc atactatgta gctactgttg taagagcata cagaatggca atagatgaat
    51781 tttacagagc cccagaaaac tggaagttca atccaatgtg gatggaagaa ctaaaaaaag
    51841 gtagtcatag acattttaca tcaggttttt acttgaataa accaacaaca gaagaccaaa
    51901 attatcaatc agcatcatat gttagaaact atgattttat aggtatagtt agagaaacag
    51961 aagatgaaga tggtcttatt gttgttgagc aaagaaataa aatgtgtgtt ggtgatgaaa
    52021 tagaagttat gggaccatat aaagaaacta tgtttactaa aattgaagaa atgtacaatg
    52081 aagaaggaga agctatagaa tcagctcctc atcctagaca aatagtgaag ttaaagctat
    52141 cagtaaaagt tggtaaagat tatatgctta gaaaagttat agaagaaaag gttgaagaat
    52201 aataaaaaat tgataaaaat agtattagcc ccttagaaat tgtcaataat gaaattatct
    52261 aaggggtgat ttcatgtcaa aaaagaagac tccatttttg aaaaaagtgg gaaagagaag
    52321 ctggtgtata tttactataa ttttaataat ttatagtgtt ttaatttata ggctagttga
    52381 tatacaagta ttaaaaggtg ataaatacaa acaaagtgtt gaatcacaaa gcgttgagaa
    52441 agtcgagtta aatagtggaa gaggaataat ctatgataga aacaacaaga agcttacaga
    52501 tacaagtaaa tctcaggtat taattgttga aaaagaaaaa ttaaataata actataaaat
    52561 tttggaactt attaagaaag ctactaagat gaatgattta gatatatata aagctgtaca
    52621 agagcaattg acacgcccta taatacagat tcagactaaa aatatcgata agagtatgaa
    52681 aaaagaactt gagaaaaatg gaataatggt agaagaaaaa actatgaggt acgcaaaaga
    52741 tggtttatta tcacatacaa taggatatat aaaagaagat gataaatcag gtcaatcagg
    52801 aattgaaaag agtatggata gtgtacttag aaattcaaat gaaaagtata taagtgcttt
    52861 taaagctgga gatgcaggaa atgaaaaatc tctaaacata ctaaagggaa gtgtaaaaac
    52921 tgttgataat aaggataaag atagacatct aaaaactact attgattata acattcaaaa
    52981 aaaattagaa caaatactaa ataaagaaga aaatcctaca gcagcaataa tctcggaagc
    53041 atctacaggt gaaattcttg caatgtgctc tagacctaat tttgaccaaa atgacatatc
    53101 aaaaagttta aaaggaaaaa atggggaatt tgaaaataga gttataaaag cgacatatcc
    53161 accagggtct gtatttaaga tggtagtttt attttctgca cttgaaaatg gtgttataga
    53221 tgagaactat acttacaact gtacaggtaa gactaaagtt gggaatacta atgaaatact
    53281 gcgatgtaat aaaagagatg gtcatggttt ccaaaactta agacaagctt tttctaattc
    53341 atgcaatcct gcatttttag atatagctat gaaacttgga aaagaaaaga tactcaaatc
    53401 tgctgaaaaa ttacatttat ttgaaaaggt tgatattggt cttgatgaag aaaaaattag
    53461 agaagctcca aaaaacatat caataagaaa tttagctata ggtcaagaaa atatagaatt
    53521 tacaccactt caaataaatc aaatgactca aataatagct aataatggaa cattcaaacc
    53581 actatactta tataaaagtc ttgtggacaa taatatgaat acaataaaaa cttataagtc
    53641 ttcaaaaaaa gaagaactta tttctcctta tgtgtgtaca caagttaaag aatatatgaa
    53701 gtctgtatct aggattggta cagctaaaga cctaaaagat attgaaggtg gttgtggagt
    53761 taaaactggt acagctcaaa gtagtttaaa taaaaaagct atagaccacg gatggataac
    53821 aggattttat cctgaagaaa gacctaagta tgtaataact gtgttagtag aaggaactca
    53881 aaaaggaaat aaatctgcaa cacctatatt taaggaaata tgtgaaagta taaaataaat
    53941 tatgtactat aaaatttaag gatatatcgt aactttgatt ttagatagaa aactttgtct
    54001 aatgatgtac gatatatcct tttaaaatat agtattaact ttcttgaaca agctcttttt
    54061 ctagtttttt aagagctttt ttttcgattc tagatacata tgaccttgat atatcgagtt
    54121 tttgtgcaat ttctctttgt gtcttatagc cataaggagt taacccatat cttaactgga
    54181 caatttctct ttctctagga gtaagaattt tgtttagttg ttcgtaaagt tttccaactt
    54241 gaacttttag ttcaacttca tcaagaacat agttagcctc tgtgccaagt atatctaaaa
    54301 gacttatttc attacacact actctttata tgttattggt attactggtc ttcatgtaat
    54361 ttcaaatgaa ccattaattc aaaagaatct agtttttgtt ctttagactt ataataaatt
    54421 gctttatcaa ttacagattt taggagttta ttttttccaa gggaatctga atcatgataa
    54481 agtgataaca cagttttaat atcttctatt attttagaat tgtctttttt aacagtactc
    54541 aatctatttt ttactgtctt aattgaatct ttaatttctt cagttcttga acttatattg
    54601 ttggaccttt caataaatgt atcttcatca tatattccac gttctaataa atcatataac
    54661 ttattttttt gtttacctaa ttcttttaat tcagtttcta agccctttaa gactcgtttt
    54721 tgaaattcta tattactatc tttattattt ttatctaagt ctaaatcaca tgcttttaat
    54781 tgatattttt ttaccgtgtc ttccaaagat tttaaaatgg cagcttcaat aaattcaaat
    54841 cgagaacttt tattgcaacc aggatggtaa caaattatat agtcgtggtc tgcatatggt
    54901 ctatatacta atggtctacc acaatttttg caaatcacta ttcctgcaag tggatttttt
    54961 atttgattac catagctgac atgaccgttt tttttaagta tattttgagc ttttttccat
    55021 gttatttcac ttattaaagg ttcatgcttt ccttttgctt caatccattc atctttaggt
    55081 cttagcttaa cagtttttat tttatttgga tttttagatt ttttatagtc ctttttttgc
    55141 cactgaatgt agccacaata taccttattt tttattatat ttgtaattgc agaattgtac
    55201 caattagcac ctgtagcagt tttaatgcct aagttattta ggtattttga tattttacta
    55261 caacccatat cttcattaat atataagtca aagatagtcc taactacatc agcatcctta
    55321 tttggtacaa gtattctttc ttttctacct gttgttacag catcatatcc aaatggagca
    55381 gaagttccta tgaaattccc ttcttcaact gattttattc tacctctttg cattcttctt
    55441 gatataagtt ttaattcttt tcttgccata aatgcttcaa attctgaata ctcctcatca
    55501 aattcatctg ttaggtcgta tgtttttcta ggtgtgatga ttttggtttt actttctttg
    55561 aatgtttcta atatgatacc ttggtctttc atgtctccac gaccaagtct gtctaagtcc
    55621 attactaaga ctgcatcata tttattttct tcaacttctt ttagaagttc aagcattttg
    55681 ggtctatgta ttatactttc accagaaact aattcttctt ttatctcaat tacatctaag
    55741 ttttgttctt ttgctagttt tagtagggtt gatttatgtc tacttaaagt ttcaaattcg
    55801 ccttgttttt ctgcctcttc atcagcacgg gattttctta agtatatggc aacactcata
    55861 ataagtaatc acactcctta tgcactatta tatagtaaat caatagagtt taaaatatag
    55921 aaaataagca tgtcaaaagt taatttaact ttcaacatac ttattttcta taatattaat
    55981 gccttataat cctttaatat catttagatt agtttttgca tattcattat ccgggttaag
    56041 ttcaagcgcc ttattgtaag attctctagc tttattaaat tctgatttac tcataaaaat
    56101 aacacctctt aaattgtaag catcactagc gtctatttta taagcttcat tatttttatc
    56161 tagatattct aaggatttat caattgactt taaagcctca tcatatcgtt gtaactctat
    56221 ataaatatat gattgtgtat aatatgcaag tggattttta tcatcaattt ctatagcttt
    56281 tttgatatca atagcagctt tattataatc cttatcttca tctttttttg tttctgacct
    56341 agcgattttg acataattag agctaagagc tcttttaaag tctttatgcc ataataattt
    56401 atattcatta tcttcgatta taaaatatct atcaaaattt gcagtatctt ctttattttc
    56461 agtgatatac tttatagtat cagtttcttt tattttaaca agatttgtgt actctttatt
    56521 agaatatttg tatttctcta ctttattttc ttcaccaagt ttataatctt ttagggtttg
    56581 aagtttaaat aaagtatctt gatattcttc aaatttttct agactgacac tttccttact
    56641 gtctttactt aataaattat atgctacttc gtagtctttg atttctatac tatcaaggta
    56701 ctttttagct acttctgtag cactttcttt tggagcacat ccaaccattg atatcataat
    56761 taaaaatgat actaatattg ctgttatttt tttactgatt atcataatat aatccccctt
    56821 atattgtctc tcactaattt tattttatca ccatattcta ccaaagtcat taatttaaga
    56881 atgatatttt tcattaatat tatattaagt aataataatt tttaaaattt atggtaacat
    56941 tttcctgaaa agtattatat aatttaggtg aaaatattta agagaaggag atattatgaa
    57001 aattaaaaaa ttattaacaa tagggttatc tttatctata tttattgcta gttgccctat
    57061 atctgcaaat gctcttgata aaattgaatc aattaaaggt gcagataaat acgaaactgc
    57121 tggtataatt gcagacaaac aaaactatac tacagctata ttaattaatg cagacagtac
    57181 aatggctgat ggattaagtg caagtggact tgcaggagct ataaacgctc caattctttt
    57241 gactaagaaa aataacatac caaatgctac acttaaaaga ttagaaaagg caaaaaaagt
    57301 atatataatt ggaggagaaa actctattaa taaatatact gaaactgttc taaaaggcaa
    57361 aggaatagag attaaaagac ttcaaggtag tgatagaatt aagacaagct ataatgttgc
    57421 aaaagaaata aattcaatta acaaagtaaa taaagtcata ttgactaatg cttttaaagg
    57481 agagcctgat gcaatgagtg ctgcacctgt agcagttaga gataaagcag ctatagtatt
    57541 aactgatgga aaaagtgttc catttaatac aactggtgta gaaagttatg taataggtgg
    57601 tacttcatca atgaatgaca aactagttaa ggatactaat tcaactagat taggtgggac
    57661 agatagatat gatactaaca aaaagataat aaataaattt tatagtggag taaaagaatt
    57721 ttatatagca agtggtactg atttagtgta tgctcttgtt ggttctacca ttgctaaaaa
    57781 taatgctatt gtgttagttg gtaatgatag taataaaagt gtattgaaaa gtacaattaa
    57841 attaactgct ataggaaatt tgagtgatag tatattacag cagtgtttaa atgtaacaaa
    57901 aaatataggc gattctaata ctggaagaga atatacagta gaagatgctg ctaatgcagt
    57961 gaaaaaacta ttaggtgtga agaaatttta ttacgaacct aattattcag aagatgaaga
    58021 tttaatattt ctactagata aaaattttga tttttcagga aaagagtact atgtatttgc
    58081 ttacattaaa cagggtgcag agggtgatgt tagatactgt gtagctaaaa atgatatgag
    58141 caaagtttat acatatgatg ttaatggtaa tatgaaagta tgtaatccag atgattataa
    58201 aaataaagga agtataacag aggaacaagc aaaacagata gcattaaaag aatgtgctaa
    58261 gagacataaa gtcagtatca ataacttagt agttgatatg atagatttta gagatgatat
    58321 atatagtggt aatgtctatg ctgttaaaat caattcaaaa aatagtaatg gtacttttgg
    58381 ttggtttttt atagatatag aaactgggaa aatttataat taaaattaga acttttaaac
    58441 aataattata taatttacca taatatgtta taatattttt agcaagaaga tttaacttac
    58501 aatctataga gtggagttca tgtacaaaag attatcctcc caacgattcg aagggaggtg
    58561 agttcatgga taactttttg tttaatgttt tagctagttt aacagctagt gtggtagttt
    58621 acttaatcag taaactattc aaaaaagcaa aaagccactc tcgcatgaac tatacccctc
    58681 ttaggagaca gttgaaatat gtaaaactgt ct
//
LOCUS       DAMKPG010000012        56069 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMKPG010000012 DAMKPG010000000
VERSION     DAMKPG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56069
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.014"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..1745
                     /locus_tag="PC621_000816"
     CDS             <1..1745
                     /locus_tag="PC621_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108002.1"
                     /translation="ENKNEITKLKTSIEKIEEEKKVIKKSIEDIDVNIFNLNSEKDRI
                     NRHIDDTESKINKFKIYEPIKLENAKLEELEIKYKKYLEDPTKKEIETLNYEISVCLK
                     RIESITIELEEFRQLISSDNFENIPILFTIEELNKRIVLNKSKVDELRKLKYSIKSDI
                     DKNQGSLNTKREEILKKFNGKEPLSTSLIVNFNFEERKIDIKKDIKDIGEIIEGNRTT
                     CQNLEQELRDLKKYKVERKAITYQPEDIFIESKKLQSKLQNVEYNLIKSINSYKNTIL
                     EIKEFNYKHMEKYKNFIETLNVNKDNYSRQKEQVEIVLLVIREEISKLEKHSEQLRKE
                     KTIISRQIKDYVMDCIDEFKIINKLGKHKGQALFNISLPKSDKIENSLSLVDMLMDEI
                     VEEANLGDVEQKINTFYILNKTLNIGRIPVSVIKYEINRNETIKWNSINNETTGGQRF
                     CISFIVTILLMEYKRYDRNAIVDESKYKGKVLLMDNPFGETSQQDFLKEIFELAKKFN
                     VQIISYTHVTNASIRAMFNKIYLMTVEKTTSNKEFVDINEIKQNKTDELVYMSKFYIG
                     NKEELIQEDIFSLI"
     gene            1847..2056
                     /locus_tag="PC621_000817"
     CDS             1847..2056
                     /locus_tag="PC621_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108003.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            2452..2592
                     /locus_tag="PC621_000818"
     CDS             2452..2592
                     /locus_tag="PC621_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108004.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            2730..3692
                     /locus_tag="PC621_000819"
     CDS             2730..3692
                     /locus_tag="PC621_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF4108005.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            3770..4399
                     /locus_tag="PC621_000820"
     CDS             3770..4399
                     /locus_tag="PC621_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF4108006.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            5230..5646
                     /locus_tag="PC621_000821"
     CDS             5230..5646
                     /locus_tag="PC621_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108007.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            5647..5859
                     /locus_tag="PC621_000822"
     CDS             5647..5859
                     /locus_tag="PC621_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108008.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            6494..7537
                     /locus_tag="PC621_000823"
     CDS             6494..7537
                     /locus_tag="PC621_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF4108009.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            7798..8672
                     /locus_tag="PC621_000824"
                     /pseudo
     CDS             7798..8672
                     /locus_tag="PC621_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8691..9683
                     /locus_tag="PC621_000825"
     CDS             8691..9683
                     /locus_tag="PC621_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108010.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            9685..11379
                     /locus_tag="PC621_000826"
     CDS             9685..11379
                     /locus_tag="PC621_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF4108011.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            11930..12808
                     /locus_tag="PC621_000827"
     CDS             11930..12808
                     /locus_tag="PC621_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF4108012.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            12844..14739
                     /locus_tag="PC621_000828"
     CDS             12844..14739
                     /locus_tag="PC621_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4108013.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            15007..16914
                     /locus_tag="PC621_000829"
     CDS             15007..16914
                     /locus_tag="PC621_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4108014.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            16904..17353
                     /locus_tag="PC621_000830"
     CDS             16904..17353
                     /locus_tag="PC621_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4108015.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            17453..18340
                     /locus_tag="PC621_000831"
     CDS             17453..18340
                     /locus_tag="PC621_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4108016.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            18745..20193
                     /locus_tag="PC621_000832"
     CDS             18745..20193
                     /locus_tag="PC621_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF4108017.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            20219..22609
                     /locus_tag="PC621_000833"
     CDS             20219..22609
                     /locus_tag="PC621_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF4108018.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            22611..23282
                     /gene="pgmB"
                     /locus_tag="PC621_000834"
     CDS             22611..23282
                     /gene="pgmB"
                     /locus_tag="PC621_000834"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF4108019.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            23321..24721
                     /locus_tag="PC621_000835"
     CDS             23321..24721
                     /locus_tag="PC621_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4108020.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            25038..25880
                     /locus_tag="PC621_000836"
     CDS             25038..25880
                     /locus_tag="PC621_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4108021.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            26025..27953
                     /locus_tag="PC621_000837"
     CDS             26025..27953
                     /locus_tag="PC621_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4108022.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            28014..29441
                     /gene="ascB"
                     /locus_tag="PC621_000838"
     CDS             28014..29441
                     /gene="ascB"
                     /locus_tag="PC621_000838"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF4108023.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            29852..30628
                     /locus_tag="PC621_000839"
     CDS             29852..30628
                     /locus_tag="PC621_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4108024.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            30644..31009
                     /locus_tag="PC621_000840"
     CDS             30644..31009
                     /locus_tag="PC621_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4108025.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(31175..31924)
                     /locus_tag="PC621_000841"
     CDS             complement(31175..31924)
                     /locus_tag="PC621_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF4108026.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(32369..32926)
                     /locus_tag="PC621_000842"
     CDS             complement(32369..32926)
                     /locus_tag="PC621_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF4108027.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            33178..34476
                     /locus_tag="PC621_000843"
     CDS             33178..34476
                     /locus_tag="PC621_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HDF4108028.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            34448..36397
                     /locus_tag="PC621_000844"
     CDS             34448..36397
                     /locus_tag="PC621_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF4108029.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            36834..36971
                     /locus_tag="PC621_000845"
     CDS             36834..36971
                     /locus_tag="PC621_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108030.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            37199..37573
                     /locus_tag="PC621_000846"
     CDS             37199..37573
                     /locus_tag="PC621_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDF4108031.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            37589..38236
                     /locus_tag="PC621_000847"
     CDS             37589..38236
                     /locus_tag="PC621_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108032.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            38616..39695
                     /locus_tag="PC621_000848"
     CDS             38616..39695
                     /locus_tag="PC621_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF4108033.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            40517..40730
                     /locus_tag="PC621_000849"
                     /pseudo
     CDS             40517..40730
                     /locus_tag="PC621_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            40842..42455
                     /locus_tag="PC621_000850"
     CDS             40842..42455
                     /locus_tag="PC621_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4108034.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            42484..42807
                     /locus_tag="PC621_000851"
                     /pseudo
     CDS             42484..42807
                     /locus_tag="PC621_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            42839..44026
                     /locus_tag="PC621_000852"
     CDS             42839..44026
                     /locus_tag="PC621_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF4108035.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            44218..44622
                     /locus_tag="PC621_000853"
     CDS             44218..44622
                     /locus_tag="PC621_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF4108036.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            45192..46052
                     /locus_tag="PC621_000854"
     CDS             45192..46052
                     /locus_tag="PC621_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4108037.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            46042..47913
                     /locus_tag="PC621_000855"
     CDS             46042..47913
                     /locus_tag="PC621_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4108038.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            47933..49387
                     /locus_tag="PC621_000856"
     CDS             47933..49387
                     /locus_tag="PC621_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF4108039.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            50411..51619
                     /locus_tag="PC621_000857"
     CDS             50411..51619
                     /locus_tag="PC621_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108040.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            51650..53029
                     /locus_tag="PC621_000858"
     CDS             51650..53029
                     /locus_tag="PC621_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108041.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            53135..54325
                     /locus_tag="PC621_000859"
     CDS             53135..54325
                     /locus_tag="PC621_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108042.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(54453..55517)
                     /locus_tag="PC621_000860"
     CDS             complement(54453..55517)
                     /locus_tag="PC621_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108043.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(55665..>56069)
                     /locus_tag="PC621_000861"
     CDS             complement(55665..>56069)
                     /locus_tag="PC621_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4108044.1"
                     /translation="RLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRRL
                     SRLISDVSWSEFIRQLEYKANWHGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        21685 a         6293 c         9974 g        18117 t
ORIGIN      
        1 tagaaaataa aaatgaaata actaagttaa aaacaagtat tgaaaaaatc gaagaagaaa
       61 aaaaagttat taaaaaatca atagaagata ttgatgttaa tatatttaat ttaaattcag
      121 aaaaagatag gataaatagg catatagatg atacagaaag caagataaat aaatttaaga
      181 tttatgaacc tattaaactt gaaaatgcta aactagaaga attagaaata aaatacaaga
      241 aatacttaga agaccctaca aaaaaagaga ttgaaacatt aaattatgaa atctcagttt
      301 gcttaaaaag aatagagagt attactatag agttagaaga atttagacaa cttattagta
      361 gtgataattt tgaaaatata cctatattat tcacaataga agaacttaat aaaagaatag
      421 ttttaaataa aagtaaggtt gatgaactac gaaaacttaa atatagtatt aaaagcgaca
      481 tagataaaaa tcaaggttca ttaaacacta agagggagga gatacttaaa aaatttaatg
      541 gaaaagaacc tttatctact agcttaatag tgaactttaa ttttgaagaa agaaaaatag
      601 atataaaaaa agatataaag gatattggtg aaattataga aggtaataga actacttgtc
      661 aaaatctaga acaggaatta agagacttaa agaaatataa agtagaaaga aaagctataa
      721 cctatcaacc tgaagatatt tttattgaaa gtaaaaaact acaatcaaaa ctgcaaaatg
      781 ttgaatataa tttaattaaa tctataaata gctataaaaa tacaatatta gaaataaaag
      841 aatttaatta taagcacatg gaaaaataca aaaactttat agaaacttta aatgtaaata
      901 aagataatta ctctcgacaa aaagaacaag tagaaatagt attactagtt ataagagaag
      961 aaataagcaa attagaaaaa catagcgaac aactaagaaa agaaaagact ataatatcaa
     1021 ggcaaataaa agattatgtt atggactgta ttgatgaatt taaaatcata aataaacttg
     1081 gtaaacataa aggacaagcc ttattcaaca tatcactacc taaatctgat aaaatagaaa
     1141 atagtttgag cttagtagat atgctaatgg atgaaattgt agaagaagca aacctaggtg
     1201 atgttgaaca aaaaataaat accttttata ttttaaataa gactcttaat atagggagaa
     1261 ttcctgtatc agttataaaa tatgaaataa atagaaatga aactataaag tggaactcta
     1321 ttaataacga aacaacagga ggacagagat tttgtatctc atttattgtg acaatattgt
     1381 tgatggaata taaaagatat gatagaaatg caatagttga tgaaagtaaa tataagggta
     1441 aggtactact tatggataat ccttttggag aaacgtcgca gcaagatttc cttaaagaaa
     1501 tatttgaact tgctaaaaaa ttcaacgttc aaattatatc ctatactcat gtaacaaatg
     1561 cttctataag agcaatgttt aataagattt atttaatgac agttgaaaaa actacttcta
     1621 ataaagaatt tgtggatatt aatgagatta agcaaaataa aactgatgaa ttggtgtata
     1681 tgagtaaatt ttatataggt aataaagaag aattaattca agaggatata tttagtttaa
     1741 tataaataac aagtaaacaa ataaagtgat tactatcagt gaggcaggta gaaactgaat
     1801 tactttgtga ctcattacga ctcttggggt aaaattgtgt ataaatgtga agaatttcgt
     1861 gattgtcagt acaaaagcag ctttattaag ggtaataata gtaagactcc tcttgaaaat
     1921 aggactaaaa acttacaagt atctaagtta tttcatgaaa aaagaaaaat taaagagaat
     1981 tataagacca gagggatgtg aacttagagt gaatagaagt atacaagtag aaagagctat
     2041 cgcagaacaa atttaatttt atcatacgat agcctcttgt tattttagtg atgaaatcca
     2101 tcaagcatac cataactaca gaaaaagagg cttttctgat ggtatattgg gttcttttag
     2161 agtgtgtgat ttgctacatg ttcattagga taataatagt cagtaaatga tattactgat
     2221 agaatagata taataaacta atctagttcc atattgtaat gtttattaaa gagtatactt
     2281 ttatgattat aatagctatt ttctttgaag cttattgtac aagagatttg aatatatcaa
     2341 atttggatat aggtataatt ctcttagcag ttttatttat acagtcttta tctaaattag
     2401 tgttgtatat actttgataa aagggggata tgtaactaag ttgtaatatt gatgaaaata
     2461 aaaattaaaa aatatagaga aaaactcctt atgatacaaa atgaacttgc taaatcagtt
     2521 ggggtaagac gagagactat tgtacactta gaaaatggat taatgtacca taaaggtatc
     2581 caagataaat agttaaaaaa ctagataaga ataaggagcc tttacagaac aaatttagtg
     2641 tgctcgtgcg atagcccttt ttttataaaa attaatctaa taacctatat aattaaattt
     2701 ataaaacata tatgaaaggg taaaactata tggctaaaat tgataatatg atgtccatat
     2761 tatggatgtt aaactcagac aaaaaagtta ccgcaaaaca aatatctgaa aaactagaaa
     2821 taaatataag aactgtctac agacacattg atgcattatc tgctagtgga gtacctataa
     2881 tttcagatac aggtcataat ggtggatata cacttttgaa taatttcatc aaagcacctc
     2941 tttttttcga tacagaagaa caaactgcgt tacttcatgc agctatattt gctaaagaag
     3001 agggaagttt cttagatgac acactagaca aagcaacctc aaagttaaaa atgtattcaa
     3061 atcaaaaaca agaagaaact ttaacaaaac atttaatagg ttttgaagta ataaaaccaa
     3121 ctggaaaaat atccatagaa tctgtactta aaaagttaaa aaatagtata gcaaatgaat
     3181 tatctgtaga aatcaactat cgtacagctc atgaagaaaa atcaaaatgt aggattataa
     3241 atccatatgg aattgtacgt tggaataata attggtacat agtagcattt tgtcaaatga
     3301 gaaatgagat tcgtagtttt agggtagata gaattagtag tgtcatagaa acatcaaata
     3361 cttttaatag gccaaaagaa ttttcagcaa gtgaattttt tataagaggg attatggatg
     3421 aaacaaaaga tgaggataat cttatatcat tagttataga aggaaagatg aatgcattgg
     3481 ataacttatg tcagcattgg cttctaggat attatttaaa agaaaggact tcaaataaag
     3541 caatatttaa acttcaagaa gagataatat ttacatatgt accgaatata cttatacctt
     3601 atagaaaatc tatacaaata attgaaccat taagccttag ggataaaatg gttgaagatt
     3661 tactcgaatt aattaatcat tatcaaattt aataacttaa ctgacagagt atgtcagtta
     3721 agttatttta caattaggta tattaattaa ttatagaagg agcaaaaaaa tgaaaaagag
     3781 aaaagtatat atgtatgtat tcgatactat gtcagattgg gaagttggat atctaactgc
     3841 tgaattaaat acaggaagat attttaaaaa aggattaaaa ccactaaaag taattacagt
     3901 aggagttgat aaaaatccta ttataacaat gggtggatta aaagttttac ctgagattac
     3961 aattgacgag cttaatattg aaagtaatga cttattaatt ttgccagggg gaaatacttg
     4021 gacatctata ggtcatgaga aaatattaga aaaatctaaa gaggctttgg aacaaaggtc
     4081 tatagttgca gctatttgtg gtgctacttt agggttagct aaaacaggtt tactagattt
     4141 tagaaatcat acaagtaatg atttagaatt tttaaaaatg gtgatactaa gttattctgg
     4201 agaaaaatat tataaaatgg aatctgcggt taatgatgaa aatttaatta ctgcttctgg
     4261 aattgcacca ttagaattta cagtacaagt tttaaaagtt ttagatgttt ttaaagaaga
     4321 agcattaagt tcttggttga acctttataa gacgcatgac tctaaatatt ttttcgaact
     4381 aatgaattgt aataaataaa tttaagattt aattttaaat tcaaatttta aattactttt
     4441 tctatggcaa tagataagta gtttagaatt ttatcatata tataatttgt atttatacca
     4501 acacaaatag aaatgatagt tccaataata aaatttataa ttagttccat atggagtaca
     4561 tttctttcct actaatataa ctaagaatat atgaaaccac catagattgt agttttttct
     4621 gttgtattat atcatgggat agtaaagtga cgtatatttt tgatgaaaac ctaggattgt
     4681 atatgaaatg aattaatcct gtgtattaat cttgagattc gtgtctaatg atttgtaaaa
     4741 ataaataatg aaatacttag aaatacttgt aaatgctagt caatattggc tagtattttt
     4801 tattttagta atgaaatccc caactgtact tcatccacaa aaaaagagat atttttgagg
     4861 gtgtattgga gtgaacttag gctgtataat tatacatttc aatatcttat attaattagg
     4921 ataatagttg gtaaatgata tgattgaaac aatagatata ataaactaat ctagtactgt
     4981 actgcttact aaagaatata tttttatggt ataagaattt tttagagcgt tgattctata
     5041 ggagtgaata aaaaagtaat tttataatat tatttgtaac cattagatgt ctattatgaa
     5101 atcttttatg gactatattt gtaaaaatat tattaagtat ttacaatatt ttcttgcaat
     5161 ttatttggaa aggttatata tttataacat gaagtgttat aaatatataa cctttaaggt
     5221 ggattttata tgtctaaatc agaaactggt gtaaaatcaa aactcatatt ggaattactt
     5281 gtaataactt atctttttat agctgtatta gctactaagt atatttttaa aggtccaagt
     5341 actgcatctc ttccaggact gagctggtta atagtctttg gatgctattc aatttataat
     5401 aaaaaatata atgatttagt tgatgaatca tctaaattta tactatcaaa ggttaatcag
     5461 attgcaataa caataatgat gtattctata gttataatag ctattttctt tgcaacacct
     5521 tatataagta atataaatat atcaaacttg gatactggta taattcttat tgcagtttta
     5581 tttatacaat cgttatctaa attaattttg tatatatact ttgataaaag ggggatatgt
     5641 aactaagtgg caatatcgat gaaaacaaaa attaaagaat atagagaaaa actccttatg
     5701 acacaaaacg aacttgctaa atcagttggg gtaagacgtg agactattgt acacttagaa
     5761 aatggaaaat ataatccttc tttaaagtta gcgatggata ttgctaaagt attcgggacc
     5821 acagtagaaa atttgtttga atttattgaa gaagattagt agataggata taaatattta
     5881 taagattgtt aataaaatat agttatttaa ttattaattt tgttaaaaat atttataaat
     5941 aatataatgt atgagtaaag ttaggttgta attattttaa gataagaaga ctactgtaaa
     6001 tgataaaaat tagtgaatat acagccaatt aataaataat agataaaact ttaaagctta
     6061 gatgtgagga tgagatatgg tcttacagta gttcacactt ttaattaaga cttaaaatgg
     6121 aaaccataca catacataca ctatttactg aaagtggact agataaaaaa ataataagtg
     6181 atttaagaaa gtatctcata tactatataa gtattatttt aggtgaccta tttataggtc
     6241 attttttatg tatataaatg ataataatac taatatttaa aataggtttt aaagaaataa
     6301 aatatccaga ttgaaaaatg gattaatgtt aataaaaaaa ttatatgtat aaaaataggt
     6361 ataattatag aaaaaaacaa taataaaata agctgttata gaaaaataca attatataga
     6421 ttgttgttga atcattgact gcaagaataa taacatttaa aatgaacatg gagaatatgg
     6481 gggaggaatc aatatgaaga gaaaaatagc agttgtttta ttaactgtag caatgttatt
     6541 atcacttgta gcttgtagca atgataacac atcaaaaagt gataaattag aggatacatt
     6601 agtagtatat actacacatg gagaggatat gctaggagca atttctgaag aatttacaaa
     6661 ggaaacagga gttaatgttg aatttataaa cttaaaaggt gaactagcag accgtgttag
     6721 aagtgaaaaa gataatccgc aagcagatat aatgtttggt ggagatacat ctacatacat
     6781 gcaattaaaa gaagaaggat tatatgaaaa gactgaacca agctggaata atgaattaga
     6841 caagtcttac aaagattcag atggatattg gtatggaact ataaaaacac cagtaatgat
     6901 gttttataac acaaaactat tatcagctga taaagcgcca aaagattgga gtgatttaac
     6961 aaaagaagaa tacaaagact taatagttac tagagattca ttatcatcat ctatgcgttc
     7021 tactattagt aacttaatca attactactc aaatactaag agtgaagaag aagcttggaa
     7081 gtatcttgag gatttaaata agaatataaa gaattactac aatagtggaa gtgtcatgta
     7141 ttcagcaata ggtaaaggtg aagctacaat atctgttgca gtattaagtg atataataga
     7201 gaataaagaa aaaaataata tgccattaga agttgtagat gcagagagcg gttctgtagt
     7261 aatcacagac tgtgtagcag ctctaaaaaa tgcaccacat ccaaatgcag cagctaaatt
     7321 tgtagatttt gttggaagtg ctaaggttca agctatggtt gcaaatgatt ttaatcgtat
     7381 tccaacatta gattctgcac ttaaagattc accaaagtgg atgtcaaaag aatataaagc
     7441 tatggatgta gattggtctg taattggaaa aaatcagtct aactggattg aaaagtggga
     7501 aacagatata atagataaca ataaaactat aaaatagcct aattaaataa tttaatatta
     7561 tatacaaaaa tagtgaggat tttcctcgct attttacgta taaggaaact atagtatctc
     7621 ataattttgt ataattcata caacaaaatg atactaatac atacttatta aataaaatag
     7681 tttttgagta actgatgaag taattgacta tataaagtga tttgtagaca tagtctctca
     7741 aataaagtga attttttatt atgcaaaact tttaattata gtaaaagtac aatttatatg
     7801 ggcaaggaga taaaaatgga actaaaaatt agtgattata aattattatg taataaaggt
     7861 agtggcgctt ttaacgaaga tatcgtaggt ttatcacctt atggggcatg ggtacttgat
     7921 ggagctacag gtttaaataa taaaaattta gtctctacag aaagtgatgc caaatggtat
     7981 gtaagttggt ggaatagtta tcttcatgag aatatggaaa gagaagatag cttaaaagta
     8041 attattaaag aaggcattga ggagataaca aaagagtact acaaattaac agatgataaa
     8101 gaagtagaag caatagatat tccttcttgt tcatgttcta tattaaagtt ttataaagat
     8161 aagatagagt atttactttt aggagattgt actttattag ttaacaataa tgaatctgtg
     8221 aaaattttaa aagatgagac tttatgtgaa cttgataaaa aagtatttga ttttatgaaa
     8281 gaagccaaag atgctacaaa ccttacttta aaggagagaa aagccgaggt aatgcgatta
     8341 taatagagaa tagacttaag aaaaatactg tggggggata ttggactttg gaattttcta
     8401 aagaggccgt tgacaattgt gtaagtggat ttattgatat agaatataaa atgaaaatta
     8461 tgatggctag tgatggtttt tcatgtggat atgatagata taagattttt gataaaaatg
     8521 aaatgatgaa aatagcagag attaaaggta tagattatat atatagaaag gttagggaac
     8581 tagaaaagga tgatgaaatg gcaatagagt ttcctagatt taaaatctat gatgattcat
     8641 catgtgtata tttagagatt tataaatatt agataattta tggagacgat atgaagaata
     8701 tagaattaaa agaaattaat cattattatg gaaaagaaaa agtattacat gatattaata
     8761 tatctattaa ggaaggagag ttttttactt tactaggtcc ttctggatgt ggaaaaacga
     8821 ctattttaag aataattggt ggatttataa aaccttcttc tggaagtatt tatgttggtg
     8881 ataaaaacat taccaattta gaaccagaaa atagaaatat gggaactgtt tttcaaaact
     8941 atgcactgtt tccaaatatg acagttgaag aaaatgtatc ttatggattg aaaataaaaa
     9001 agctgtctaa aaaaattata aaggaaaaat gtgataatta tttagagctg tcaggaatga
     9061 aagattttag gaaaaaaaag attgatgaat tatctggtgg acagcaacaa agggtggcag
     9121 tagcaaggtc attagcgact gagcctacta tgcttttatt agatgagcca atgtctaatc
     9181 tagatatagc tttaagaatc aagatgagag aagaaataag agagatacag caaaagacag
     9241 gtattactac tttatttatt acccatgatc aacaagaagc cttagctatt tctgatagaa
     9301 tcgctgtaat ggataagggt aaagttcttc aaattggtgt gccaatagaa gtttacaaaa
     9361 gcccattgaa tgattttgta gcaaactttg taggtacttc aaactgtatt ggaaaagaag
     9421 attatgttaa ttttaatatt gaaaaagaga caaagcctta tatttataaa agacctgaag
     9481 aaatgatatt attgcaaaat acaaatcaaa gtggttttgt aaaagtaaaa gtagaatcta
     9541 agaattttct aggagcaatt ttagaatata cagtatcaaa tgatgggaaa aaatatcaag
     9601 taactgagct taatagactg aataatagca ataaattaaa cgtaggggat atggcatatt
     9661 taggtgtgct acaaggtgac taatatgatt aaaaaaataa accgtagtaa atttacatct
     9721 aaaaatgtta tttttattgt tttatacagt atattatttt ggattttgat tggatatatt
     9781 atcataccaa taggcaatac ttttgtacaa gcattccaag gtaaaggtgg atattctttt
     9841 gaagttttta aagaatattt ttcaaatact aataatttaa gggtagtaag caatactata
     9901 attttgggat tgggttctgt gttcgtatgt ggaattatgg gaatatcact tgccttatat
     9961 atgacatttg tttgtgataa acataagaaa atcatacata ttcttttact aagtccaatg
    10021 atgattcctg gagtaattat ggtaattgca tgtattcaga tttatggaga aagtggaata
    10081 gttacaaaat ctatagaaat tttactcccc tttatcaaaa aaccttatga ttttagtgga
    10141 ttaaagggaa ttttatttgt ggttgcttat actcagtatg tatattttta tttaaatgta
    10201 tatgttgcct taaaatatgt ggattactca acagttgaag ctgcaaaagg aatgggagca
    10261 tcaaagctaa gagtattttt tgatgtaata tggcctgtta ttacacctgc tgttataact
    10321 tctactatta taacttttgc atcagggatt agttctttct cagctccaaa tcttataggt
    10381 ggtggtttta aagttttaag cactcaaatt gttaggtcta aggctaataa ccatatggat
    10441 atagcttcag ttcaagtaat tactttatta ctaatgggga ttttatttat gattttatta
    10501 cagtattatg gcaaaaaata tggagtagtg ccatccttaa aatctcaaag ttataaagtt
    10561 cataatagaa aaaaagactt atttacattt gtatgtaagg ttattattga aatacaaatt
    10621 attatgatta tgcttcctat agttactatt gtgtatttat cctttgtaag cacacattca
    10681 attatgacaa ccatatttcc tagagacttt acactggaaa actatcagat gattttccaa
    10741 aaatcacgtg tattacagcc tatattaaat agtttcaaaa tgtctttaat ggcagtagca
    10801 gtaggattga taattactgt accagtatca tatttagtaa ttaaaaagga tactgcatat
    10861 aatcgactag caaaatttat tataatgtta ccttggagta tgcctgcaag cgccattgca
    10921 gttaacctaa ttaatacatt taatcaaaaa agtatatttg cttttaatga atccttaata
    10981 ggaggttttt atattgtacc tatagcatat acaatttatg ctctaccatt attattttca
    11041 tccaatgagg ttgctatgaa gtcaataaat ttaggactag aagaatcatc aaggagttta
    11101 ggtgctggaa ggataaaaac cttttataat gtaatagttc caaatatgat gccaggaata
    11161 atttcaggag ctatattagt attcattcgt acaattgggg aatatacaat gtcagccttg
    11221 ctttatggtg tttataatag acctatttca atttctattg taactaatat gcaagaattt
    11281 aagataggag tatctatggc ttatggtgta ttagtaattg gtatatgcta tatagcatta
    11341 ggaataattt ttaaattaga tagaaaaaga tttacataag ataacaaagg ctaatttata
    11401 aattggtgct tagaaaacca aatcacttta ttagctaaag atgaatttga agggtgacac
    11461 tttggtgttt tatgtaaagt ttatggaagt aaaacattaa ataatcgtga gtatgattcc
    11521 tataaatcgt atatactatt gaaatccaag aaaaggtatt ggctaaatag ctaatatctt
    11581 tttaattttt tgactcccat atagcattaa ctattacatg agtatcttta aatttatagt
    11641 taaagtatct taattgttta ttataaaaat ataggatggc taaagcacat taaattaaaa
    11701 cacttaaaaa gagaagatgt acatgagaaa tataattaac tatgatacag acttctaaaa
    11761 attatttagt gaaaaggaac ttagagtgta taataaaagt tccttttcaa taaggaaaac
    11821 gattgttatt ttctgagctt cattaccctg atattattta aatataaaca acaacaaaat
    11881 taacaagaag taaattcaac ctaaaattta aaatatcggg aggtacaata tgttaatcaa
    11941 tatgaaggaa atgctaaaag tggcacaaga aaatcaattt gctgtaccag catttaatat
    12001 aggaagtggg caaatactta aagcagtagt acagggtgct aacgaaaaaa atgcaccagt
    12061 aatacttgca atacacccaa atgagctaag ttttctaggc gatagctttg tagttagttg
    12121 catagaagaa gcaaataaga gtaaagtacc tatggtaata cacttagatc atggggaaaa
    12181 taaagaacaa atattaaggg caataagatg tggatttaca tcagtaatga ttgatggctc
    12241 acatttacca tatgaagaga atgtagctat atctagggaa atagtagaaa tagcaaaagg
    12301 attaaatgtg tccgtagaag gagaacttgg aacaatagga acaacaggaa catcaagtga
    12361 aggtggtact gatgaaataa tatatacaga ttcaaaatta gctaaggatt ttgttgaaaa
    12421 aacaggagta gacacactag ctattgcaat tggtacagca catggtatat atccaaaagg
    12481 ttttaaacca gagcttaaac tagatttatt aaaagaaata agagaagtag tagatatacc
    12541 tttagttctt catggtggtt catcaaatcc agatgaagaa atagcacaag cagttaaact
    12601 aggagtttgt aaaataaata tttcgagtga tgttaagtcg gcatattata agaagtgcag
    12661 agaactatta gaacaaaacc caagcttata tgagccagat actatatatc caccatgtat
    12721 aaaagctgca agagaagtta tagagtttaa aatgaatcta ttcaatgcta tagataagtt
    12781 aaagtgcttt tataaaaata aaaattaatt atgaggtaac aaattaaaag gaggggagaa
    12841 tttatgatag gtaaaatact acgaagcgat tgtattgtac tagataaaca attacaaaca
    12901 aaaaaagatg taattaatta tatggcaaat catttgtacc atggaaatta tataaataat
    12961 aaagaaaagt ttatagctga tattaataaa cgtgaaaaag aatcatcaac taatatgggt
    13021 aatttaatag ctatacctca tgctaagtca aatgtagtaa atcaacctgt aatgatgtat
    13081 ttaagaacag atagagagat tacagataat gatggtcaaa aacttaggtc attttttatg
    13141 atagcagttc cagaatcagg ttcagatgaa catcttaaga ttatatctaa actagctaca
    13201 tatctaatgg aagaaaattt tcttaagcat ctactaagtg cagaaactcc atacgaaata
    13261 ttaagtatat ttaataataa agctgcagaa tcaggagatt ttagtaataa tcaaaataaa
    13321 ggtcttatag ttgggataac aggatgtcca acaggtatag ctcatacttt tatggcaaaa
    13381 aaagcattag aggaagcagg gcaagaactt ggatatgaag ttaaaataga aactcatgga
    13441 gctataggtg tagaaaataa gttgactaaa gaagatataa aaagagctga atacgtaata
    13501 atagcttgtg ataaagaagt tcaactgagt agatttatag gaaagaaagt gctacaaaag
    13561 aaagtagtag atgctaagaa tgtttcatta gctaaaaatt taattataaa cgctgaaaat
    13621 ggaatggcta aagaaattaa accaggtgga aagactttag aagattcaaa agaaatgctt
    13681 caagggtcag agacaaaggg tgtttataag cacttaatga gtggtgtatc tgaaatgatt
    13741 ccatttgtag tgataggtgg aattggtata gccatagcat ttatgtttgg tatatttgca
    13801 tcagacccaa ctcatgaaac atacaaccct atagctggat tctttggaca acttggaggc
    13861 gattcagcat ttaagttatt tataccgata cttgctggtt ttatagctaa atcaatagca
    13921 ggaagacaag gatttcctcc tgctatgata gctggtttta tggcagttat tggaggttca
    13981 gggtttatag gtggtatggt agctggtttt gcaacaggat atatagcttt aggagtaatg
    14041 aagataacag aaaatttacc agatgtatta aaaggagtaa atgctgtatt gatatgtcca
    14101 cttttgattg catttacgag tggagctata atgttctttg tagttaatac gccagcatca
    14161 tggttaaatg aagctttaca atcttggtta caaagtatgt ctggaactaa taaagtatta
    14221 ttaggtgcaa tattagcagg gatgatggca tcagatatgg gaggacctat taataaaaca
    14281 gcttcagctt ttggattggc tatgttttca agtaatatat ttgagccatc agcagcatta
    14341 atggtaggag gaatggtacc tccattagcg atatcattag ctatgttaat attcaaaaat
    14401 aagtttacta aagaagaaag aaagtcatct ctaccaactg ctataatggg ggcatctttc
    14461 ataacagaag gcgcaatacc gtttgcagct gcagacccat taagaatgat tccagcaaat
    14521 ataattggtt cttgtatagg tggggcaatc tgtatgagtt tagatataac tttaatggca
    14581 ccacatgggg gaatatttgt aataccattt gcatgtagtg caccagttgc atatatagct
    14641 tgtatattga ttggtacagt aataacagca ttgattatag gggtagcaaa gcctactata
    14701 agaagtgaaa acagtgaagt tgaattactt ggtgcttaaa ttataaataa tagtaaaaat
    14761 aagtaatgga ataacaacaa gtatatagaa taataataag gatatttaga atcagttaac
    14821 tacttctaaa tatccttaat ttttattttg aaattatatt atctattata tcaatttagt
    14881 aaggtgtatc ataaattctt ttatgataca gaagtatctt agtaatttta catagttagt
    14941 aaatatgtta aaatagtact taatataaac atagaaatat ataaacatag gtagggaggg
    15001 ctaagtatgc ttgtgtcgag gcaaattgaa tttcttaaat tgatactaaa cgaaaataaa
    15061 tatagaccta taaaatactt taaagataaa ttaaatgtat ctgataaaac tttacaaaaa
    15121 gatttgaaga tgattgagaa atatttagaa acttttaata taaaaattga tataaaaaga
    15181 ggctatggaa tattaataga agatttagca aagaagaaca tagacttaat taatagttta
    15241 aatatacaat cgaaggaaaa taaatcttta tcagtagagc agagaagaat ggagatttta
    15301 aagtatttat tactaaattc aaataatata acttcaataa atcagttagc agataaatat
    15361 tatgtaagta aaacttctat agtaaatgat tttaaatata tagaggcatg gataaaagaa
    15421 tacaatttaa aacttaataa aactttagaa ggaactaaga ttataggtaa agaagtggat
    15481 ataaggaaat cgattgccaa aatgatggat gatttattag atgaaaatta tgaacaacat
    15541 gatatatctg agttaacaag attagattct gctacattta gtgcattgat aaatttattt
    15601 gatattgatt ctattatatt tgtagaaact ataattacag aattagaata caatttagga
    15661 tatactataa atcagcccta ctatataaat ttaattaccc atatacttat atgtttaaag
    15721 agaatcgaag aaggtaatca aatagaatct caggaagaaa gagaaatttc tgtagataat
    15781 ttggacgagt tagtatataa aaatgtcact acacttatag ataaaataga aaatagatac
    15841 agagtaaaaa taacgaaaga tgaaattatg tacatatata tttttttggt atcatctgga
    15901 tttagtagcg aaaataatag tagttataat gatgaatcag atatattaag tgaaagtgaa
    15961 aaagtttcat ctataatgat agagaatatg tcacaatttt taaatataaa tctcaaaaat
    16021 gatgacttac tacaaaaaag tctagcatct catgtaaggc ctatgttaaa cagattaaga
    16081 tatgatatcc aaataaaaaa cccgttgtta ggagagatac aagagcgatt ttcagatgtg
    16141 ctgggattat gtatgatgac tataaatatt atgacagagt atgataatct gaagaatatt
    16201 agtatagatg aagtttcgta tcttgccaca tattttcaag cggcaataga aagaaatatg
    16261 agtagtaaaa gagtaatagt agtttgtcat acaggatatg gaacatctca gctccttgca
    16321 gccagattaa aaagagaatt tcctgaatgg actatagttg atatagttcc tatgcatcag
    16381 ttgagtaagc gaaatttaga tgatgtagac tttgtaatat ctactgtaaa cttagatttg
    16441 aaagataaat tacatatagt tgtatcagta ctattaatgg aaagtgatat taataatata
    16501 aaaaatgctt tactttctaa acctaaaaaa agtatgaact tagatacaaa cttattagga
    16561 atgtatttag aagataatat atattttaat tttgatgtag ctcagatgga aagtttaata
    16621 ggtgtgaatt tagggagtaa atatgaatct atttcattag gaaaagattt gaatatatat
    16681 atacataagc aaagtaaatt aaataaaggg ataatgaata tcaataatgt tactagaacg
    16741 attgatattt atttagaaat tagtaatcaa agttttttaa agaatatttt aatggaaata
    16801 tcaaatttat ataggcaaaa gaactatata aactatataa tagattgcaa gagcaaagag
    16861 gatattaaaa tattatttaa gaataatcta ttggagaaaa caaatggaaa ttagagaaat
    16921 attaacagaa gaaagcatta ttttagattt gaaatcaact acaaaagaag gcataataca
    16981 ggaattagta gataaattat acgatacagg aagtataact ttaaaaagta agtttataaa
    17041 ggcattaaaa catagagaaa aaataggctc tactagtata ggttatcaaa tggccatgcc
    17101 acatggaaaa acaaaatatg taaagaaggc atccatagta ataggtaaat ctaaaaaagg
    17161 tgtagattgg ccaagtatag atggcttacc tgtaaaggta ataattatgt ttgctatgcc
    17221 acaatctgga ggcaatgaag atatgatatt aatatctaaa atagcttaca ttttaggtgg
    17281 taaaaatata agtgaaagac ttttaaatgt acatacaaaa aaagaaatat tagatatatt
    17341 ttatgaagtg taggtattgt ataaattata aatttgaggt ttttaaaata aaatgtgtat
    17401 tcttttaaat agtatagatt aatttttaga cactagagag gagaggatat ctatggcaat
    17461 atatttagaa atgcctaaat taatacatga atttcctttt cgtagtctat caaatgaagg
    17521 agaagtatta acaacaccac actggcataa ggaaatagaa atattactta taacagaagg
    17581 tgtagtaaat ctatttataa atgacaagcc tatgcaatta aaaaaaggcg agatagtgat
    17641 aataaaagga ggagatattc attatatact gccatcaccg ggaagtgaaa gattagtatt
    17701 tcaatttgat atatcttttt ttaatgattt aattttatta aatgaggaaa aagaaagctt
    17761 aattaattta ttcagtagta taaaaaaatg tagtagagat tggtcagcaa aaataagaaa
    17821 gtcagttttc gatatattga tagatatcta caatgaagac ctatacaaaa tagaaggata
    17881 tagttatgca ataaaaggta aaatgtataa tttaattcct atattatata gacagattcc
    17941 tagagaaatt gaagaaaata cagtagaata tgaaataaac tctaaagaga tattagagag
    18001 attgaacaat atattcaaat atgtagaaaa aaattataaa caacctataa aattagaaga
    18061 tgtgtcttat gaagtaggat ttagtgtata ttattttact aaatttttca aaaaaaatac
    18121 aggaaaaaca tttataactt ttttaaatga atatagatta gaaaaagcaa agtggatatt
    18181 acttaataac aatatatcca ttgtagattt ggttgaagaa gttggttttg gaagtacaaa
    18241 aactttttac agagtattta aagaaaaaat tggagtctct ccattagaat ttaaaaaaag
    18301 atataatagc ttggagaaaa aagggggtgc ctcaaaatga acttagttca tgaggcactt
    18361 tcttttatat gaaatcaaat atattttcag tatttcaaca atgaaaattc cattcattta
    18421 taaagtctaa attttagttc tcaatacaat ctctatttcg aaataaatcc ttttatatat
    18481 ataaatatac agcaatatat gagaataaga taacaataat gaggatataa aaatgtattt
    18541 gctatatata taattatata catagatatc taatattgat atttaatatg aaagtattga
    18601 aaatagacaa tattaattat agtgagtaaa aaatataatt ggatttttaa attgaattat
    18661 ttaaaaggta tagtatgaaa aaaatcttat aactaaatat gagatagaaa ttataaaact
    18721 ttatactgtg aaagaggtag atgcatgaaa ttagggaaag attttttatg gggaggggca
    18781 atttcagcca atcaaagtga aggattttat ttagatggag gaaaaggttt atctcagttt
    18841 gataaactcc cattaaatga taagagatta aaagatgtat gtttagatga ggataatatt
    18901 ttaaatgaaa ataatcaata ctatccaagt catataggga ttaaattttg tgaaaattac
    18961 aaagaagata tagcaatgtt agcagaaata ggttttaaat gttttagatt ttctatatct
    19021 tggtcaagaa tattcccaac aggagaagaa agtgaaccaa atgaaaaggg attacaactt
    19081 tatgataata taataaaaga attaaaaaaa tataatatag agccattaat aacgataagc
    19141 cactttgata taccttattc tctagttgaa aaatattctg ggtgggctga taggagagtg
    19201 gttaatttat atgtaaaata tgctgaaatt ttgatgaaaa gatataaaga tgatgtcaaa
    19261 tactggattc cttttaacga aatgaatatg ataattcata ttccatatat aggtgctggg
    19321 ttaactttta tagctgatga aaatcaactt cagaaaaaat atcaagcagc acaccatcaa
    19381 ttattagcta atgcaaaaac tatagaaata ggaaggtata taaataaaga atttaagttt
    19441 gggtgtatgt tagctgctgg aaaaacatat ccatacacat gtaaaccaga agatgtattt
    19501 gcaactttag agcatgaaag gcataatttg tttttttctg atgttcaagt taggggtgtg
    19561 tatccaagct ttatgaatta ttatttaaaa tcaaatcaaa taaaattaat aataaaagag
    19621 gaagatttat caacacttaa ggataatact gtagattttg tatcttttag ctattattct
    19681 agtgcttgta gttcagctag agctgaaggt ttagaaaaat caaaggcaaa cggaccagaa
    19741 accataaaaa atccatatct tccaaaatca aatagtgtat ggcaaaacga ccctgtaggg
    19801 ttaagaataa ctatgaatca gttatatgaa aggtatcagt tacctttatt tatagtagaa
    19861 aatggactgg gtactcaaga tattgtaaat gaagatggaa aaataattga tgattatcgt
    19921 atagattaca tgaaagaaca tattaaaaat atgattcttg gagtaaatga agatggaata
    19981 gagcttatag gatatcttat gtggggatgt atagatgtaa ccagtgtaag tgaaggaaag
    20041 atgacaaaac gatatggcat aattcatgtt gattctgatg actatggaag aggctcattt
    20101 aaaagaacta aaaaacaaag tgctttttgg tataaaaaag taatagaaac agatggagaa
    20161 attttattta atgatatgga aactcatgca taattcattt taagaaagga gtgttattat
    20221 gagtttaata tttactacta aggaccaact tatagagcaa gggtttagta aggatacaat
    20281 aaaatccaat gaaacgttgt ttacactagc taatggacat ttaggagtta gagggaatat
    20341 tgaagagtcc gattttaata atgagtatgt agataatatg ggtacatatg taaatggatt
    20401 ttatgaagaa tctccaatta catatggaga aagcgcttat ggctatgcaa aacaaaatca
    20461 gactatctgt aagttaccta atgctcatat agtaaatttc tctattgaag gagattgctt
    20521 tgatttatca aaaggtatta caagtgaaca tactaggata ctagatttaa atgaaggaat
    20581 tgttaaacgt tcatttatat gggaaaactc tcgtggtaga aaagttaaag tatctataga
    20641 aagattagta tcacaagata tagaagaatt aatgatgatt tcatatacaa taacaccatt
    20701 gaattttaat ggaaaaataa aatttaaaaa taaaatggaa aatagtatgg agaagtttaa
    20761 ttgtgaaaaa gtagatgacc caagagtggc atctataaat aagaagcaat ttaaaattaa
    20821 tgtaaaaaaa tataacaata agtattttat gaatttagtt acagaaagaa gtagattagg
    20881 gttatggtgt gggtttgatt catatattac ttgtggaaat attacaaatg agacctataa
    20941 tgtttcagat gaatgtattg agcgagaaat agaagtctat gccaaaaaag atgaaaggtt
    21001 aaatttagaa attgttgtag gctatagtag agtatattct gatttaaatc taaatgaaga
    21061 acgtttaaaa tcattagaat gtctggagtc gttaaaatgt ttagaatcag aagcacattt
    21121 aaaatcatta gaattggttt taagagaagc atgtaatatt ggatttgata aagtcaaaaa
    21181 tattcagaga aagaaaatga atgagttttg gacaacctct gatgtagaga ttgaaggcga
    21241 taaaactctt caactgggta taaaaaccaa tttatttcat atttatcaat cagcaggaag
    21301 agatggaaag actaatatat cagcaaaagg tcttacaggt gatggctatg aggggcatta
    21361 tttctgggat acagaaatgt atatattacc attttttata tatactcaac catccattgc
    21421 tagagcttta ttagattata gatactcaat cttagatgca tcaagagaaa gagctaggga
    21481 acttggaata aataaaggtt gcttatattc atggagaact ataaatggta atgaatgcag
    21541 tgcatattat ccagcaggaa cagcacagtt tcatataaat gcagatatag cttatggtat
    21601 tagaacgtat tttgaagcaa ctaatgatga agaatttatg atgcaaaatg gcttagaaat
    21661 tcttatagaa acagcaagat tttgggttga atttggagat tatatagaat taaaaaataa
    21721 taaattctgt ataaatggag taacaggacc agatgaatat acagcaatag taaacaataa
    21781 ttactacact aatttaatgg ctaaatataa tttaatatat gcagtacaga tggtagataa
    21841 atttaagcat aatttatttt caaatgaagt atttagaaaa gttaaattac aagaatatga
    21901 agtaaaagac tggataaatg ccgctcaaaa tatttattta ccatatgatg aagagaagaa
    21961 aataagcaaa caagacgatt catttttaga aaaatcagta tgggatttta aaaatacacc
    22021 aaaagaaaaa tatccattac ttctcaatta tcatccatta acaatatata gatatcaagt
    22081 aaataaacaa gcagatactg tactttcagc gttgcttttc cctgatgagt ttagcttaga
    22141 acaaaagaag agagattttg aatactatga aaaaataact actcatgatt catctttgtc
    22201 aaggtcaata tttggaatga tggcaagtga aattggaaat catgaaaaag cttataacta
    22261 ttttatggac acagcattaa tggatattaa taacctacaa aaaaatacaa gtgatggaat
    22321 acatgctgct aatatgggtg gaacatggat gagtttagta tatggatttg caggaatgaa
    22381 aataaaaaag gataaattaa gttttgaacc aaggatacct aaacactgga aaaatattaa
    22441 atttaatata ttatttagag ataatttagt ttctgtaaat atagaagaat ataaatctta
    22501 ttataagtta atcaaaggta aaaacataag catattacat gataataaag aaataaaatt
    22561 atcaaagaac ataatagaag ttaataattg ttttaaaggg agtaattaat atgaaagcag
    22621 tcttatttga tcttgatgga gttataacag atactgctaa gtatcatttt gaagcttggc
    22681 atgagatagc aaaatcgcta gatatagaaa tagatgaaac ttttaatgaa aaattaaaag
    22741 gcataagcag ggaagaatca ttagataaaa tattgttatc tgctaataaa gtagatagtt
    22801 atactaaaga ggaaaagtta aaggtgtgtg aagaaaaaaa taacatttat ataaagttaa
    22861 taaactcatt aacatataaa gatatattac cagggataaa tgagcttata aaagaactta
    22921 aaaaaaataa tgtgaaattg ggaatagcat ctgctagtaa gaatgcacct aggatattag
    22981 aatctttggg aataaaagat gaatttgatt gtatagtaaa tccatcctta ttagaaaggg
    23041 gaaaaccata tcctgatatt tttattgaag ggagcaagat gctaaattta aaacaatatg
    23101 attgtgtagg aatagaagat tcttattcag gtattaaagc tataaatgct gccaatatga
    23161 catcaattgg tgtggggaaa aaagaagtac taggaaaatg tgaccatatt gtagaagata
    23221 ctagcttatt aaattacaat acactattac aagcttggag taaaaagatt aatttggatt
    23281 aaaattatat taaaaattat tttaaaagag aggataaggt atgaagaata aatatgaaga
    23341 agtagctcaa ttgataattg atggacttgg aggaaaggat aatataattg acttaactca
    23401 ttgtatgaca cgattaaggt tcatcttaaa agatgaaaag aaagtcaatg cagataaatt
    23461 aaaatctata gataaggtag ttggagtaaa ctcaacttca actcaatatc aggtcataat
    23521 aggtaatgag gtaggtgttg tatttaatgc aataataaat aaggggataa ataccacagg
    23581 agatattaaa agcgatataa aaaatgaaaa taaaaaaaat agtattttaa ataatataat
    23641 agatacaata actggttgta tgacacctat gataccagcc cttacagcat ctgggatgat
    23701 aaaagttata ttatctcttt gtaccacttt taattggcta tcatcaaatt caagtacata
    23761 tagagtatta gatataatag gagatgtagc attttacttt atgcctataa tacttgcaat
    23821 aaatgcagct aaaaaattta atgttaatac atcaatagca gttatagtag taggtgtgtt
    23881 tttacatcca aatttctctg catgggtatc aagtggggac cctatatcat ttataggagt
    23941 accaattcaa ggtgttatat atgcagcatc tgttatccct gcacttttaa cagtatggat
    24001 gatgtcatat atagaaaaat tcattaataa attaacacct agcatgttaa aaacaatttt
    24061 aaatccaact ttagtattat taataagtgc gccaatagca ttaatagtaa ttggaccaat
    24121 aggaaatttc ttaggagagg gattagctag tataataaat ttactacaag gaaggttagg
    24181 atttataatg gtttgtttat tagcagctgc aatgccattt atcgttatga cagggatgca
    24241 tcatgcattg actcctatat tcatatcagc atttgcaact acaggacaag aatcgctaat
    24301 attaatatct caagtttgtg ctaatttagc gcaaggtggc gcgacattag ctgtagcatt
    24361 aagaagcaaa gatagtaaga tgaaacagtt agcatcagct gcatggatat cagctatcat
    24421 gggtataact gaaccagctc tttatggagt tacattgaaa cttaaaaaac ctgctatgtt
    24481 agcatctata ggagcaggaa tcgcagggtt atttgcagga attactcatg tgactttata
    24541 tgttccacaa aatagtataa tggcagtact tggattatca ggggaaaagg gaatttcaaa
    24601 tatagtaaat ggacttatag ttatatttat agcattggtt gctccattta ttatgacata
    24661 tgtttttgga gttgaaaaaa atgttaagga aagtacagat aatgtagtag aggaagtata
    24721 atattaacta tttacattaa tagaattttt attattattg actataactt gttttgtatt
    24781 tatattgatg caaagaaata agttaatatt aatgataata attattataa ctgacaaaaa
    24841 gcaacacaaa agattgatga agtatatgga aataaaaagt tgacaaaata tgtctgatgt
    24901 tttatcatat taattggaat taggtagtaa ttgtttaagt acaagctaag cttattcatt
    24961 aataatagca gtatcctagg aaggagtatg cccattatta tggataagct ttttttatgc
    25021 ttaaaaggag gataatcatg ataattaata agatattaaa caataatgtt gtaatcacat
    25081 tagatgataa tgatgaagaa gttatcgtta tgggtaaagg aattggttat caaaaatcaa
    25141 aaggcaatct aatagataaa actaaagtaa ataaagtatt tagaatatca aataaagaaa
    25201 tatcaaacaa actacaagaa ttactcaata atattcctat agaacacatg aaattatcaa
    25261 gtgaaattat agaatacgca caaataaagt taaacaagaa gctaaatgaa agtatttaca
    25321 tatccttaag tgaccataca tattctgcaa ttcaaagaat gaaagaagga ataaatgtaa
    25381 aaaatgcaat tctttgggaa attaaaagat tttataaaga agagtttgaa attggaatga
    25441 aagcactaga tattattgaa aacaaaacag gtataaaatt accagaagat gaggctggat
    25501 ttatagcctt tcatatagtg aatgctcagt taagtgaagg tcatactttg gcatctgata
    25561 ttacgaagtt aatacaagaa gtacttagca tagttaggta tcattttagg atagaatttc
    25621 atgaagaatc agtgttttat tatagattta taatgcattt aaagtttttt gcgcaaagat
    25681 tgcttttaga tagtgcacat gaaggtgaga cagacaaaga attactaagc attattaaat
    25741 ctaaatataa taaagagttt gaatgtgtag ttaaaattaa aaatttcatt aaaaaacaat
    25801 ataactacat attaacagat gatgagctta tatatttaac aatacattta gctaaagtag
    25861 ttaaagactc aaatatgtaa gtgaaataat ttttcagtag tatattaaga atgattgaaa
    25921 ggagtgatac taacaaaatt atacctatgg ttggatggta acattgagtt ggccaaacct
    25981 tcaaatttca aaaaattaat ttaaacgaaa tatggaggag aaaaatggga aaatacaaac
    26041 aattagcaca agagatagta aacaatgttg gtggaaaaga aaatattagt ggattagtac
    26101 actgtattac aagattacgt tttactttaa aagatgaaag tatagcaaat gacaatgtat
    26161 taaaaaacat ggaaggtatt gttactgtaa tgaaaagtgg aggacaatat caagtagtta
    26221 taggaaatca tgttgaagct gtctataaag atgtagttga aataattggt ttagatgatt
    26281 caagtacaag ttcagaaact aaaaaatcag gtaatatact agataaaggt atagatataa
    26341 tatcaggaat ttttcagcca gtgttaggaa taatggcagc ttgtggtatg ctgaaaggat
    26401 ttaatgcatt atttgtagca atgggattat atagtgatgt atctggtggt tatatggtaa
    26461 taaatgcagc aggagatgca ttatttacat tccttccttt gttcttagga tatacttcag
    26521 ctaagaagtt tggattaaaa ccaatgttgg gattagcact aggagctgca gtatgttatc
    26581 ctgctataca aggtagcagt atatcagcag gagcagatgt gatgtacact ttatttaaag
    26641 gaacaatgtt tgcatcacca gtatatattg atttctttgg actaccaatt gtatcaattg
    26701 attatactgg aacagtcgta ccaattatat ttatagtgta ttttgcttct aggtgtgaaa
    26761 aattatttaa taagtttgtt ccagatttag ttaagttttt ctttgtgcca atgcttacat
    26821 tattaataac agtgcctgtt gcacttatag ttataggtcc tatcgcaaca tttggttcta
    26881 cacttatttc acaaattgta atttcaataa gagattttag tccactattg gcaggtgcta
    26941 tagtagggtt tacttggcaa attcttgtta tttttggtat gcactgggga tttattcctg
    27001 tatatataaa caatgttatg actttaggat atgataatgt tatgatgcca ttctttgctt
    27061 gtacatttgc aagttcggca gttgtgttgg caatattttt caagactaaa gataaaaaac
    27121 taaaagagat gacaatacca aactttatat ctggtatatt tggagttaca gagccagcaa
    27181 tttatggaat attattgcct ttaaagaaac catttataat aagttgtatt gctagtggga
    27241 ttggaggcgc tttctacgga ttctttaatc ttagaaaatt tataactgga ggtatgggaa
    27301 tatttgaact tccagcaatg attgaaccag atgggggaat gggcaactta atagtagctc
    27361 tttctggtat agctatatca atggtcgtag cttttgtatt aactatgatt ttatataaag
    27421 atgagaaagt agaagagtct aaaaaagtaa gaaataaggg gaaagaagaa ataaaagaag
    27481 tcaagtctac taaattagaa agagaaatag tagcaagtcc tataaaaggt gaagtattaa
    27541 aattaagtga tatagaggat gcagcatttg catctggtgt actaggtcaa ggggtggcaa
    27601 taatacctag tgatggaaaa gtagttgctc cagtagatgg tgtagttaca accttatttc
    27661 ctagtctaca tgctataggg attctttctg atacaggtgt tgaagtgctt attcatattg
    27721 gattaaatac tattcaatta gaagggagag gttttgaggc atgtataaaa caaggtgata
    27781 gaattacaaa aggtcaaact atattaaact ttgatgttga tgcaataaaa gagttggggt
    27841 atagtactgt aactccaatt gttattacaa attcatcaca atttttagat gtagtagaaa
    27901 cagaaagtaa aaatattgaa ttagaagata atctaattac agtcttattt tagtaaaaat
    27961 tgaggcatta gcataagcta gtgctttggt tataatttaa ggaggctttg actatgagtt
    28021 ttaaggatga ttttttatgg ggaggagcta ctgcagccaa tcaatgtgaa ggtggataca
    28081 atgaagacga aagaggattg gcaaatgtag atgtatgtcc aacaggaaaa gatagaacag
    28141 cagttattac agggaaacta aaaatgtttg attttgatga tgagcattat tatccagcta
    28201 aaactggtat tgatatgtat cataattata aagaagatat aaagttattt gctgaaatgg
    28261 gatttaaagt atatagaatg agcattgcat ggagtcgaat atttccaaaa ggtgatgaag
    28321 aaacaccaaa tgaaaaggga cttcaatttt atgaaaatat atttaaggag tgtaaaaaat
    28381 ataaaataga gcctttagtc actattacac actttgattg tccaatgcat ttagttaaaa
    28441 agtatggtgc atggcgtaat cgaaaaatga taggtttcta tgagaaatta tgcaatgtta
    28501 tctttagaag atatagagga ttagtgaagt actggctaac ctttaatgaa ataaatatga
    28561 ttttacatgc tccatttatg ggtgctggta tttgctttga agaaggtgaa gatgttgaaa
    28621 agataaaata tcaagctgct catcatgagc ttgtagctag tgcaattgca actaaaatag
    28681 cacatgaagt tgattcaaat aacttagttg gatgtatgtt tgcagctgga agtgtatatc
    28741 cttatagttg taatcctaat gatgtatggg aagctacaaa gttagataga gaaaattact
    28801 tctttgtgga tgtacaatct aaaggtaaat atcctaacta tgcgttaaag tatatggagc
    28861 aaaaaggtat tacaccagaa atggaacctg gtgatattga attattgaat aaatatacag
    28921 tagattttat ttcattttca tattacaata gccgttgtgt tagaactgat gaaaatgcag
    28981 atgatatggc tgaaggaaac atatttacat ctgctaaaaa tccatatctt tcatacagtc
    29041 aatggggatg gccaattgac ccattaggtt tgagaattac actaaatcat gtatatgata
    29101 gatatgaaaa gccactattt attgtagaaa atggattagg tgctaaagat atagctgatg
    29161 aaaatggtta tgtagaagat gattacagaa ttaattatct aagagaacat ataaaagcta
    29221 tgcatgatgc agtttctata gatggggtcg atttattagg ttatactaca tgggcaccta
    29281 ttgatttagt aagtgctggt acaggtgaaa tggaaaagcg ttatggattt atatatgtag
    29341 atagagataa tagtggtaat ggaagtctaa gaagaatgaa aaagaaaagt tttgaatggt
    29401 ataagaaagt aattgcttca aatggtgaag atttatatta aacaagaaat ctatgttaaa
    29461 taagaaattt gtattaaata agaagagtat aaaaattaat aataagtata atttttaact
    29521 tttttaacta tagaaaatca atacagaatt attttacatt gtatttatgc aatagtatga
    29581 ttcacaaata aaattttaga aaataaaatt gttaagaagt atgggaagta agtgcaattt
    29641 actttgtaat aaaagatgaa aaaggcgagc ttaggtttta agcttgtctt ttttattatg
    29701 tataaactat gatactttaa aattctatga aaaatttatt taatagagaa attaaaaatt
    29761 taatggtaat caaattcttt accaatgaat ataaaaaatg tctacgtgtt ataatagttt
    29821 aaactaataa acgaaaggta gatgaatgaa tatgaaatac caacatataa tagaagttga
    29881 caaagaactt tgcattggat gtgggctttg taaaaatgat tgtccagtaa acaatataat
    29941 tatagaaaat aaaaagtcag taattaaaaa acaagattgt ctaatgtgtg gacattgtgc
    30001 agcaatatgt cccacaaaag ctattacctt gacaggattt gatgaacctc ctatagaact
    30061 tacaaataag ccaaagctag attcagatga attactaatg gcaattaaat ctagaagaag
    30121 tatacgtaaa tttaaagaca aagaagtttc ttcagaaatt gtaaagcaaa ttattgaggc
    30181 aggtaggtat acccctagtg ctaaaaattc acaagatgta tcatatattg ttttagacaa
    30241 taaaaagtca atttatgaaa acgaagcagt gaaatttttt agaaaaataa aacctattgc
    30301 aaatatagct attaaatatt caaaagaagt tgagattgac gataactttt tctttaagca
    30361 tgctcctatt gcaataatga taattacaaa agataagatt agtggttcac ttgcagcatc
    30421 aaatatggca ttaatggcag agtcttatgg tctaggtgtt ttatttagtg gatatttttc
    30481 agatgtagca aataattcgc caaaattaaa aaagctttta ggtctaaaac gcagtaatca
    30541 tgttcttaca acattagtaa ttggataccc agatgttaaa tatagacgta ctgcacagaa
    30601 agaagtagca actgttagat acttataaga gagggtggat tttatggaaa aagaatgtca
    30661 acaaagaatg caagaaataa catctaaatt taaagaatgt cgcaaagcta tctctgctat
    30721 gggtgatgag actagacaat tgattttaat tgctctctta gagagtgatt ttaatggtat
    30781 acgagtggga gaaatcacaa aaaaaacaca tctttctcgt cctgctgtat ctcatcatct
    30841 taagatatta aaagaagcag agattgttaa tttgcgtaga gaaggaacga aaaattatta
    30901 ttatcttgat tcaaaaaaat cacaatggaa gtcactaaca gagctaataa atcttatatt
    30961 tgttggaata caacatatta gtgaaaatga tataagaaaa ggtgattaaa ttatacttga
    31021 tttaatacaa aaagattgat ttaggaacta aaaagttcac tttgagacac tatcttttga
    31081 actataccca tcttaggaga cagttaaaat atgtaaaact gtataaaaca atgggtatag
    31141 ttcattttat aatggtttaa atattaagaa tatattattc taaatagtat ctttctctgt
    31201 tttgagtttt atttttatac tcaatagctt cagtggggca accacaaata catgccatac
    31261 aatgagtaca gctatttttc caaactggct ttttgttttt taaattaata ttatttaaag
    31321 gacatagctc tacacatttt ccacagccaa tgcatttatc agtataatga aatcctttgg
    31381 cactaataat tgtcttataa aaaactgtat ttattatacc acttttaatt gtacctccta
    31441 agccactagg agtaatagtt tcaaattcat tttcattgcg tatatcagaa ataatcctat
    31501 atatttgttt atctgcttct ttaatcattt gttttgatgt ttctttgtct gttgtagaaa
    31561 ataaagctat ataattctct ggcattttaa tttctgccat accttttaat tgccattcct
    31621 tgtatttaca tagtttttga atatagttta tggattttgc tgtatctccc ccacaagtca
    31681 ttacaaagta aactctgttg tctcctagaa attttgtttc ttttataaaa tctgtaacca
    31741 ctattggcaa tctccatgca taagtaggac atacgaatat gaatggttta cctgctgata
    31801 ttaattcatc tgttttttcc tcttttataa gttgatttag tgatatcagt tcatcattta
    31861 attcttgtgc aattttacga gccacatatc ggctattacc agtaccgcta aaatataaaa
    31921 tcataataat atctccctaa ctatttttta ttttaactct taatttttta tttattatag
    31981 tactttattg aaatattcta ttcaaatgta tgtaaaattt agtagttaag aaaattataa
    32041 cacattagta atttttatat tcattggtaa agaatttgat taccattaaa tttagtaaca
    32101 aaatatcaac tggattttta tataagaaga gatagtaatt ttcatgtgaa agattgtaaa
    32161 tataaagatg attttgaagg gcaataagaa gataaaattg atagcttaat aaaaaatatt
    32221 taacttttat attaactgtt ttaaatatta taattatcta tcttgaataa tcaaccatag
    32281 aagactgtat acaaagacaa agttgtgtac agtctttttt ataaatttaa tgtctataat
    32341 taaatacact attaaaatca ataagttatt atttcaaagt agtattatcc aaatttttat
    32401 gatggttaat acaatttgtt agccatttta tagcaccttg ttccctgata atagctccat
    32461 caagaactat ataatcacca aaatcattac tatcaagagg aggaacttca ctataacgct
    32521 ccatttgact gtacaagaac gtaattcttt cttggtgttg aagcaattga tgctctagta
    32581 aaataagcct agtatctata tctaaattat tagaaaaata cattctaaga cgaaaaatat
    32641 ccttaggggt tctttccatt ggctcatcct tttcaagcca tttcaagaac tcatctttac
    32701 ctttttctgt gattgtgtat aactttttct ctaatacatc accactaatt ttaatatcat
    32761 atacaataag cttttcattt accaattttt ttagttcagg atatatttga ctatgctttg
    32821 cattccaaaa ctcagctaat tgaaaattga actcctttac gatatcataa ccagtcatag
    32881 gatttctatt taataatcct aaaattgcat actttaaagt tcgcataaat acacctcttt
    32941 ctttaattac atacttatta taacacgata tatttagttt gaattaatta atatagctat
    33001 gttttaataa gaacttttaa attatgagga acaaaaaata aataaaacta tatataaaaa
    33061 tgaaattatg taaatataaa catgtaagta tttacatgtt tatatttaca tatagaaaaa
    33121 attgtgataa tcttaactaa gaaattctat ttaataaatt tatcgaggag gagagtaatg
    33181 aatttaacac aaaaaagatg gattatatta attgcaagtt gtttcatcaa tttatgtatt
    33241 ggttcaatat atgcgtggag tgtatttgca tcacctatgg cagaacattt aaatagtctt
    33301 gctggaacaa cttttacagc tgctagttta gcaattgtat ttacagttac aaactcagtt
    33361 ggacctatta ctatgattac agggggaatg ataaatgata aatttggtcc taaaaaagtt
    33421 gtatttgtag gaggattaat ttttggtata ggtatgatat tggcaggttt ctcaaaagga
    33481 ttgcctatgt tgattttatc atatgggata ataacaggtc ttggggttgg catggtttat
    33541 ggatgtacaa taagtaattc aattaaattt tttccagata aaagtggatt agttggtggt
    33601 ataacaacag cagcttatgg gtttagttca gtaataatgc ctccagttgc taattggata
    33661 attaataaat atggagtaac ttctgcattt aaatcaatag gtttagtatt tcttataatt
    33721 gtatgtgttt cttctttctt tatcaaaaaa tgtccagcag atttcacacc agaaggatgg
    33781 gtaccaccag agaaaaaagg aaaagtaaga gttgttatta ataaaaattg gaaagaaatg
    33841 ctttcaacac ctatatttta tctaatgatt ataatattaa cttgtggagc tttctatgga
    33901 ttaatgtgtg tgtcattagc atcttctcta gcaacaaata tgattgggat gacaagttca
    33961 gattcagcaa tagtggtttc aattttagcc ttgtttaata cattaggccg tattgttgca
    34021 ggatatatat cagataaaat tggtagaata aatactttat caggtgcatt agttatttca
    34081 atttttggtt tattatgttt gttattctca agagaaggag atgtagcaac attctatatt
    34141 ggaataataa ttataggtat atgttttggt tcatttatgg gaattttccc aggatttaca
    34201 gtagataaat ttggacctaa aaataatagt gtaaattatg gaatcatgtt tattggtttt
    34261 gcttttgcag gatatattgg acctgcagtg atgaaaaatg tatattctgt agatggcaat
    34321 tatcaaaggg ctttcattat agctataatt atagctttaa taggttttat attaacattt
    34381 ttatataaat atataaataa aatacaaaat ttaaagatag aaaatttaat aaagggagat
    34441 ataaaaaatg gaaagaaaat atccaaatct atgtaagcca attaaaattg gaaatgtaac
    34501 attcaaaaac agaatgtttt cagcacctat ggggggaaca gacataacag cagattgtac
    34561 aattggctca aaatcaacag ccttttacga attaagagca aaaggtggag caggtgcagt
    34621 tacaattagt gaatgtatgg ttcatccaga aacagatggt tcacatgcat atcatctaga
    34681 cttaaaaata gttgattcct tagcaagttt tacttataca gcagatgcaa ttcgccgtca
    34741 tggagcaata ccaagtgtag aactatctca ttcaggacaa tattcaggaa cttatttagt
    34801 tgacaaagat aagaagcgag gattatcaca atggggagta agtcctagtg ttcgtcctga
    34861 tggattagag ataaaagaac ttacagaaga aataattgct gaaatagtag agtcatatgg
    34921 aaatgttgca gcattggcta agagagcagg gtttgagatg attatgattc atggtggaca
    34981 tggctggcta atcaatcaat tcttatcacc ttactttaat aaaagaactg acaaatatgg
    35041 tggttcttta gaaaacagag ttcgttttgc taaagaagtt ttagatagtg ttcgtaaagc
    35101 agtaggtcca ggatttccaa tagagtttag gatgagtggt tctgaattat ttgagggagg
    35161 gtatgattta gattatggaa cagaaattgc taaattattg gaatcaagag tagatttact
    35221 tcatgtatca gcaggtacat accaaagagg gtttgcagta acacatcctt ctatgttctt
    35281 agaacatgga tgtaacgtat atcttgcaga agaaattaaa aaacatgtaa atattccagt
    35341 tgctactata ggagcattaa atgacccaga aatgatggaa gagataattg ctagtggaaa
    35401 agcagatgtt atttacatgg ctcgtgcatt actagctgac cacgaattac caagaaaagt
    35461 tatgagtaat caagatgaaa aaatagtgaa atgcctaaga tgcttcactt gtatggcaga
    35521 acgtgctacc acttctacaa gacgctgcac tgttaatcca cttattggac gtgaattaga
    35581 tggaactgaa gttgtttcag tattaaaacc taaaaaagta ttaatagcag gtggtggacc
    35641 aggaggattg caagcagcca ttacagctgt aaagcgtgga cataaagtaa tactttgtga
    35701 aaaaactaat gaattaggtg gaattttaaa gggagaacaa gcacttccat ttaagtataa
    35761 aatgtatgaa ttaggaaata cttttggaaa aatagcaaaa gatttaggtg ttgaagtacg
    35821 tttaaataca acagttacaa aagaatatgt tgaaaatgaa aatgtcgatg cattaattat
    35881 agcagtaggg tctgaacctc ttgttccacc aatagagggt cttgatggag aaaatgtagt
    35941 aatagttaat gattattacg tagaaaaaga aaaagtcact gacgaagtaa tagttttagg
    36001 tggaggatta gcaggatgtg aggctgcaat tcacttagct caagaagaga agactgttca
    36061 cttagtagag atgagagcag aacttgcccc agatgcaaat ataaggcatc gtccaatatt
    36121 attagaagaa atagaaagac aaggtatata tgtatataca gaacataagg gtttaagtgt
    36181 aacaacagaa ggtgtagtat gtatgaataa atctgggaat aaaataaata ttccaggaac
    36241 ctcagtaata tgtgcacttg gacaaaaacc aagaagagaa gtggtagaca ctttacttga
    36301 ttctgctcca tatgttgctc aaattggaga ctgtgttcgt gcatctacaa taacaaatgc
    36361 agtataccaa gggcatcatg ctgcgctaga tatatagtta aaaagacgta gatagtattg
    36421 ttttattata aaaaaggagt gtctcaaaat gaacttttta gttcatgaga cactttttta
    36481 catgaaatta aatatatttt tatcatttca acaatgaaaa aagattatct ttattttgag
    36541 acagcacttt tgttattatg attttaattc ttattgcaag ttttcatttg attgatgagt
    36601 aaaacttttt aagatgtaga agtatttaac aatgtaaatg accctaactt aaatacttta
    36661 gcgaaggttg acagatttgt gtttgctttt atctattata acttaaggaa acatgttacc
    36721 tgaacaataa atttatataa taaaagtata taaatatata tatgaatgtt aagattagtt
    36781 gatagaattt aaataataaa tccaatatta taaacatagg aggtatgaaa aatatgatta
    36841 atgagaattt aaaaagtcta agaaaaatcc accagtatac acaagaagaa ttagctgaaa
    36901 aacttaatgt ttctagacaa tcaatagcaa aatgggaaag tggagtttcc caaacaaaag
    36961 gatattactg aaataacctg tgtttacctg cttgtagaga aaagaatatt gctattaagc
    37021 cagagatact acataaatgt gttggtatct ctttttcgtt aatgcaacac agtaaattat
    37081 ttcattttcc taattatttt agactgataa gtaacttttt aaatgaagaa aagaaatgac
    37141 aatacttaaa ggagcatggt atacttaaag gagaatttca ttaaggagga aaaagagatt
    37201 gtttaatcta atagaaatgt tatttcctat aatgtttata ctgattttta taataattgt
    37261 tttcactttt gtgaaaggaa tagctacttg gtttaaaaac aataattctc ctcgcttaac
    37321 tgtgtcagca agaatagtag caaaacgtca gaataccact cataacaatc aaccaaacgc
    37381 tggtgatata tcaggagcac atggatttca tacaatttcc agcacctcgt attatgtgac
    37441 atttcaagta gatagtggtg atagaataga gctgtctgta tctggctcag agtatggagt
    37501 gcttgcagag ggagataaag gacagctgac attccaaggc acaagatatt taacatttga
    37561 tagggaaaat tgaatttaga ggtgcattat gaataagttt ttgagtcaga taaggcgtat
    37621 agataatgaa atatctatta ctaggaaaat tataaataca atatttatac tgtgttttgg
    37681 aatagtttta ggaacttttg caaaattttt agatactaca gcaagcaata ctttaccatt
    37741 tatatttgaa tatttagata ttagtaattt ttttggtcgt tttgctatat ggttattgat
    37801 tgctttgtac attgcaattt atagtcattc ttccataaga gcatcattaa atgtgttagt
    37861 atttttcatt ggaatggttt caagttatta cctatattcg tattttgttg ctggattttt
    37921 tccaaaaaat tatgcaatga tttggcttgg ttttactgtc atttctccat tgttagcttt
    37981 tatttgttgg tatgctaagg ggaaaagcaa aatatctttt atactttcag taataataat
    38041 tgcaatttta tttaatttta catttattta tggttggatt tattttgatg tgtattcaat
    38101 tttagaagtg atagtctttg gttgtgcatt aatagcttta aaacgtaaca cctttaggga
    38161 gacaacgtat atgatattga gtgcagttgt tattgctgtt atacttaata tgttagttcc
    38221 atttcatttt ggttaggcag attttaagat tattatagat agaatataaa tttcacgaac
    38281 cgccgaagcg atccgccagc ggtggcggtc gtaagacata tcgccacgcc agcttgctgg
    38341 cgaggtggcg gttcactttg gcggtttggc gagcctgcga gctttctaag cccccgttat
    38401 ctaacagggg gggactatta caaaagacaa gagcaaaaac aaatataaaa ctgctgatat
    38461 gcaacgattt ataaggttgt aagaacttat ttcactttac accctattct ttctacccga
    38521 caggtctaaa acagcatatc aatagaaaat aaatataaga tacaaggtgt atgctcctat
    38581 gagtatatgc ctttttttga ttggaggaat gaagcatgaa tgatatgaaa tttattgaaa
    38641 cattaaagca aaagagaaat gtctgtgatt attcgcaatc acgactggca caggaactac
    38701 aaatcaacag gcagaactta aatgaaattg aaaacggaaa aacaaaagcc agtaaagaaa
    38761 tgaaacatat actcttacat tatcttgatt attgtaattg tacacagcct ttcactttaa
    38821 cgattgacta tctgcgtgtt cgttttccta ccacagacgc attggaaatt ataaaaaatg
    38881 tgctggcaat gaaaagcgaa tattttatac atgaagatta cggaatgtat gcttatgaag
    38941 aacagtatat ctatggggat attagtgtaa atgcttctaa agatagttct atgggtgttt
    39001 tattggaatt aagaggtatg gggtgtcggg acttagaata tgttttacag gtaagaggga
    39061 tagactggta ttgcttttta agatgttgta tagattatca aggtgttttt aaacgcatag
    39121 atttagcagt caatgatatg ggcggattgc tggatataga aattctaagg gaacgttatt
    39181 atgctaacaa ggtatggaaa cgttcaagaa cccatgaagc agtagacagc ggaaaattat
    39241 cgggtacaca tggagatact gcaaaaactt tttatatcgg ttcaaagaat agttctatct
    39301 acttttgctt gtatgaaaaa gaaaaggaac aaaaaagcaa gggtataaaa acagatatta
    39361 agaaccgctt cgaaattcgt ttaaaaaatg gaaaagcagg acaaacggta gagcaacttg
    39421 ttttttcaag aaatcccgaa cagaccatag caagctttat tctcacacag atagattttc
    39481 ccgattatat tttatgggat atatttttag ataatgtaac gacctcactt ccgtttatta
    39541 tgacacctgt tgctgttaat atggataaaa taaaacgctg gctagaaaga caggtcatgc
    39601 cctctttatt gatgataaaa gagattgaaa agaaaacagg agcaaattat ctggaagaaa
    39661 tcgacaggca tacaaggcta acagaaaagc agtaattaaa gattaaacaa atgacaacag
    39721 acatagcaga tatgattgag aaagataccg ctgttccaca agggaatgac ggtatttttt
    39781 aatttcttca aaaaaactgc aacaaaatga ctgcttgtgt acagatatgg tgcgaggaaa
    39841 ggaaatctaa ctttttttga aaacaggtca ttaaatcggt ggctgctgtc cctatatgat
    39901 gtgaaagaag aaacgatttt taatttcaac ttaacatttg acagttttca ttccctatat
    39961 ggagcaaaga aaatctttta ttccatagtt ggcagttacg gtgttgcatg ggtagttagg
    40021 gtgtgaaaag aaaaacagat ttttttcatc accaacttag caaactgata cttgctttcc
    40081 ctatatggtg caggaaaaga gagtttaact attttcattt caacttagca actggacgtt
    40141 tgctgtacag atatgtagtg aaaagagaaa aagttttgaa tattggttac gcttcggctc
    40201 ttttccaacg cggtatatag gaaagacgat tttcttcttt catgttcttt gacaactgaa
    40261 taaagcaatt caatacgttt gacagacagc gatccattga agcagatacg ccatgacctt
    40321 ttccctgcaa aagcgagcga accctatttg tgttcctgta aaagacttgt cctcttttat
    40381 cgccatgacc tgttctgtct gataaatgat actcccgtac agccacagct tgaacgttca
    40441 gagcgtcgca cgcaatgggt acggctacat aagaactatg cagaggtgga attcctgtgg
    40501 gctatgacaa atgccgttga attgcttaga aaaaagatta tggaaagggg gtatgtgtta
    40561 ttgctgatat tgtaaaaaca atgcaagaaa agacaaataa taagcgtagc attggcaaca
    40621 gaggaaaaac aaagattgtt gtaaaagagc atttttccga aaagggaaag acaatggagg
    40681 aacttctgac tgatgttatg ctggaaaaag caaagcagac aatcgcataa aattggtgca
    40741 gttgtaagac atagattgaa tggcaaaaag tacccgtgtt atactttact tgcaagcagg
    40801 tattgtaaac acgggttagt tataaaggag gataactgac aatgaaacaa cagatttaca
    40861 atactgcact ttatcttagg ttaagccgag atgatgaatt acagggcgaa agttccagca
    40921 ttaccacaca aagaagtatg ttgcgtctat atgcaaaaga acatcatttg aatgtgattg
    40981 atgaatatat tgatgacggc tggtcgggaa caaattttga aagaccgagt tttcaaagaa
    41041 tgattgagga tatagaggca ggaaaaatca actgtgttgt aacgaaagac ctttcccgac
    41101 ttggcaggaa ctatattatg acaggacaat atacagaatt gtattttccc agccataatg
    41161 tccgttacat agcgattgac gacggtgtag acagcgagaa aggcgaaagt gagattgcac
    41221 catttaagaa catcatcaat gaatgggtgg caagagatac aagccgtaaa gtgaaatcgg
    41281 catttaagac gaaatttgca gagggtgctc attatggtgc ttatgctcca ttaggatata
    41341 agaaacaccc tgacatcaaa ggaaaactgc tggttgatga tgaaacaaaa tggattgttg
    41401 aaaaaatctt ttccctagcc tatcaaggtt atggtagtgc caaaatcaca aaggtactgc
    41461 gagaggaaaa agttccgaca gcgtcttggc tgaattttac aaggtacggt acttttgcac
    41521 atatctttga gggaaagccc gaaagcaaac gttatgagtg gacgattgct catgtcaagg
    41581 caatcttaaa aagtgaggtt tatataggca acagcgttca caatatgcag tctacggttt
    41641 cattcaagag caagaagaaa gtgcgtaagc ccgaaagtga atggtttcga gtggaaaata
    41701 cccatgaacc gattattgac aaagaagtgt tctatcgtgt gcaggaacag ataaaatcaa
    41761 gacgtagaca gacaaaggaa aaagcaacac cgatatttgc agggcttgtc aagtgtgcgg
    41821 attgtggctg gtctatgcgg tttgcaacga atacggcaaa taaaacgcct tacagttatt
    41881 atgcttgcag ttactacggg cagtttggca aaggtacttg ttctatgcac tatatccgtt
    41941 atgatgtgtt gtatcaagcc gtattgaaac gattgcagta ttgggctaag gcagtacagc
    42001 aggacgaaga aaaggtgctg aacaagatac agaaagcagg caatgcagag cgaatacggg
    42061 aaaagaagaa aaaggcaagt gcctttaaga aagccgagaa ccgacaaaat gaaattgacc
    42121 gtttgtttgc gaaaatgtat gaagatagag cctgtgagaa gataacagag cgaaattttg
    42181 tgatgttgtc tagcaagtac caaaaagaac aaatagaact ggaacagcag ataaccaacc
    42241 taagagaaga attaagtaaa atggaacagg atatgatagg tgctgaaaag tggattgagt
    42301 taatcaagga gtattccgta ccaaaggaac tgacagcacc gttattaaat gcaatgatag
    42361 aaaaaatcct tattcatgaa gcaataacga atgaggataa cgaaagaata caggaaatag
    42421 agatatatta ccgatttatt ggaaaagtag actgataaag agggttcata tctttaacta
    42481 agggaaactg gagaatctat tcctgatata ggtagttgca ttaaacttgc aaaattgtac
    42541 aaggtcaaac tagatgactt agttaatcat tcggaagaaa aaacaggtat tatagtacct
    42601 cctaaaggaa agtttttctt tggagcagta gtagttggtg aacgtggtca gattgtcatt
    42661 ccaaaagaag caagagaagt atttaatata aatgcaggag ataaattatt ggtacttgga
    42721 gatgaggaaa gaggattagg aattgttcat caacgtgatt tgataaattt tataggtcat
    42781 atgggagtag tacgtaaaga agaatgattt taaaaaatta taaaaggagg tatgaaatgt
    42841 gagtaaaata gtatttttta gcattcttgc atatggtcat actaatccta ctattgaagt
    42901 agttcgtgaa ttagtggata gaggaaatga ggtattgtat tattctttta atgaatttaa
    42961 agataaaatt gaaggtgctg gagcaaaatt tatttgttgt gataaatacc ttccagaatt
    43021 actccctgga gatgagaaaa aaattggtaa ggattttcct ggattaattg agatgatagt
    43081 agatactact atttctttag atgaaaaagt ttgtagggaa cttaaagatt ttaatccaga
    43141 ttgcattgta tcagattcat taagtttttg gggaaagtta tttgcacaaa aacttaatat
    43201 tccatatgta tgctctacaa caacttttgc atttaataaa catacagcaa agttgatgaa
    43261 acaaaacttt atagaaatta tacgtatgat ttttggtgta agacgcatta ataaaaaaat
    43321 aaagctttta caaagaaaag ggtatgaagt aaaaaatttt atttctatag tttctaatga
    43381 taatgatacg gataccattg tatatacatc aaaagaattt caaccaatgg tagaaacctt
    43441 ctctagtaag tattcatttg taggtccatc agtatcaaag ttgattatta aacctaaaga
    43501 gagaaaaaga aagttaatat atatttcgct tggtacaatt aataataaaa atgttagctt
    43561 ttataaaaac tgtataaatg catttaaaga ttctgatgtt gatgtgatta tgtctgttgg
    43621 tagaagtact gatattaaaa gtttaggaaa tataccaaat aattttgagg ttaaaaatag
    43681 tgttgaacag attgctatac ttcaaaaaac tgatgtgttt attactcatt ctgggatgaa
    43741 tagtgttaat gaaagtttat attatggagt acccatggta ttatttcctc aacattcgga
    43801 gcagagaatg gtagctaaga gagttgttca tttgggagca ggaattatgt taaaagagga
    43861 taagtccgaa agtattaaaa aagcagtatt tcaagttata aatgacaatg aatataaaga
    43921 aaatgcaact aaactatcaa aaagctttta taatgcagga ggctcaaaaa aagcagcaga
    43981 tgtaatatta cagattatat gtaatagtag acaagttgac atataataga tttgttaaaa
    44041 atatatatac atacatgcaa tttaatcaaa gtactgacag tggggtcttt taacctacca
    44101 gttggggcat atatattgat gctaaatatg agggatgtta tattttaata aaaaagataa
    44161 catccttttt ttagtgcgtt atatcaagtt ttaaataata aatgaaggag gattaatatg
    44221 aaaaaatata aagttatttt tatagtatta attataggta ttataagtgt aacagggttt
    44281 gttgtatata aaaactgcat taaaccatgg aaagatgctg aaacatatat tgataagtat
    44341 atggtaaaac aagatatttc aaaagataat ataaaaagta ttacaaaaga aaaagccaag
    44401 aaggcaagtt acgaaggaat actatacaaa gtgtattaca aagatgaccc tcgatatgag
    44461 tatcaatatt tttatagtga taattatcat gctctatatg taaataaagt aatgttacaa
    44521 atttatgatg tggaaaataa taataaactt ttaagaagta atgaggaatt aaaaagtgta
    44581 aagtatccac caatatatct taaatataag ccagataaat gatttatgac aaaatagaat
    44641 gatttttatt aaaatttatt taaaaatatc actagagtgt ttaaataaaa ctaaatttaa
    44701 gtatttatat attagtatat cttaatagca tttaaataaa actaaatttg aagctttacc
    44761 tattaatata tcttaatagt gattaaataa aactaaactt aagaatttat ttattaacat
    44821 acagttcatt atatttaaaa tcattactat gataattcat aatagtaaaa tagataatgg
    44881 ttcagacaat attgtaaata ttagtgacag taaaatagta tcaatttagt atataaactt
    44941 tgccagataa tcgcataaca gagcttgtag ttatagaata aaaaaatatc taggaaaccg
    45001 ttgacagatt tatatggata gtataacata tagaatatga aattaaacaa aaaagaattt
    45061 taattatggc gacgtagtca taattttgag tatgttactg attagcaggc agaactcaag
    45121 tatatatgtg aaaaatagag ggatactcta tacatatatg cttgagttct ttttattgaa
    45181 aggaggaata tatgaaaata attcagattt taaataataa tgtagcctta gtaaaaagag
    45241 gtaaaaatga aattattgta tttgttaaag gaattggctt taaaaaaaga gtgggacaag
    45301 taataaatga aaatgaaata gagagaacat atatactaga ttcatacgat atgctagaac
    45361 actttagtta tctactacaa aactcagact ccaataatat agttcttatt aatgaaataa
    45421 ttgaatatgg tgagaaagct ttaaatataa agtcaagtga ttatctaagc ttaaccttac
    45481 tagaccattt agagttttta cttaaaagat gtgaaaagaa tcaatttata aaaagtcctt
    45541 tggtatggaa tgttaaaaga ttttatccaa agcattttga aattggaatg catgcactaa
    45601 aattaattgg tgatagtaag ggaattaatc ttcctgatga tgaggcagta tcaatagcac
    45661 ttcattttgt aaatatggag gtaaataaag agtctcatga tagtacaatt gtagagttga
    45721 gaactttagc agatatagtt tctataataa aatatcattt taatgtagag ctagatgaaa
    45781 cttcaacaaa ttatatgaga tttactactc atctacagta ctttatccaa agaattatgt
    45841 cagatgaagc ttcaatagat gaagataatt ctatagattt atataaacaa gtaagtcaac
    45901 tttatccaaa gtcatttgga agtgttcaaa agataagaaa atatataaaa tcacaattta
    45961 atgtcgatat atctataagt gaagaaactt atttaatgtt gcatataaat agggtgactc
    46021 aaagactgga ggcaaaatca gatgaaatat aaagatttaa atgaaaatat aataaaactt
    46081 atagggggaa aagaaaatat aaagtcggta gcgcattgtg ttactcgttt aagacttact
    46141 ttaaatgata gaagtatcgc tcaaacagaa gaaattaaaa aattaaaagg agtaattgat
    46201 gtagtttcta atgatgttgc ttatcaaatt ataataggta ctcatgttgt agatgtatat
    46261 aacgagttta tgtctatgat aggtttaagt tcaagtgaat caagtataaa aagtgaaaga
    46321 aaaaagggaa ttagaggtat caaaggtata tttaattcca tctttgtggt agtctcagag
    46381 actatgactt cagtaattga agtactactt gcagctggta tactcgcaag tatattagca
    46441 cttttaaata tgtctggcat acttcagagt gatagcccta catatcaaat attagataca
    46501 ttaagaagtg ctacatttca cttcttacca gtattaattg caatagcatc agcaaaaaga
    46561 cttaatgtaa atccctatat agcagtagtg ttagcagtta cgttattatc gtcatcaata
    46621 gatggtgtag aaggattaag tttatttggt ataaatttac aggctacaac ttatgcaaac
    46681 agttttattc caatactttt aggagtatgg gcaatgggaa ttataattga aggtttgaaa
    46741 aaaatattac ctaaagcact acactatttt ttagttccag tgttatcttt aataataacc
    46801 ctatcagtta cattaactgt atttggacct attggtatgt ggattggtga tggattaaac
    46861 tatgcatgta tgtttttaat tgatacattg ggtaattgga gtgttgttgc actgtatgca
    46921 gctttacaac cactcttaat agtttttggt gcagcaaact ttactatgcc tatatcttta
    46981 aattctgtga caacattggg gtatgaccca atttttatgg gcgctgcaac aatatctgat
    47041 attgcagttt gtggtgcaat gtttggatat ttcttaaaaa ctaggaataa agaacaaaaa
    47101 caattatttg ggacagtaag ttttagtgca cttatgggag ttacagagcc ttcagtgttt
    47161 ggtgtgttta tgaaatatag aagaccattt gtagcagtaa tgataggtgg aggtataggt
    47221 ggtttaatag caggtttagc tcaagttaaa acatatggaa tggtttgggg attagctgca
    47281 ttaccaacat atcttgttgg aggagaggca tctaacttta catttatgat aataagtgta
    47341 gtagtatcat ttgtagcagc aacagcagct tcctatttac taggaatacc ttcagatgaa
    47401 aaagaagaag ttaagggtga taatgaaaaa gaattagaac taataatcaa cgagaacaat
    47461 tcaagaacta ttagtattgg aaatgttgca aaaggtcagg taattgcact tggggaagtt
    47521 aatgacaaag cattttcctc tggagcatta ggtaaagggg ttggagttat acctagtgaa
    47581 tcaaaatcaa ctgttatatc tcctgtagat ggaactataa ctatagtatt tccaactaaa
    47641 catgcatatg gtatagtgac tgatgaagga ttagaaatat taattcatat tggtatagat
    47701 actgtaaact tagatggaaa attctttgaa agtcttgtaa cacaaaatca aagggttaaa
    47761 aaaggagatg ctttagcagt atttgaatct gataaaatta tagaggaagg ctttgattct
    47821 actataataa ctgtagttac aaatactagc gattatttag atgtaatctc atcaaatgat
    47881 gaatcagaag aattattaac tgtaatagta taaattttag gagggaaaat tcatgaatat
    47941 taaagataca tttttctggg gaggaagtat tgcagcccat caatgtgaag gttcatggga
    48001 tagtgataac aaaggacctg caattatgga ttttgtaact aagggctctt atgaaacgcc
    48061 aagagtaatt actgataaaa tagaagaaaa attggattat ccatctcata atggaataga
    48121 tttttataat agatataaag aagatatagc tttatttaaa gaaatgggat ttagtgcatt
    48181 aagaatatct atagattggt caagaatttt tccaaatgga gatgatgaaa atcctaatga
    48241 attagggatt aaatactatg aaggtctgat agatacatta atagaaaata atatagaacc
    48301 tattgtaaca ctatatcatt ttgaattacc tatgaattta gtacataaat atggttcatg
    48361 gaataacaga aaacttatgg atttgtattt aaagtacagt gagactgtaa taagaaggtt
    48421 tgatgataaa gtaaagtact ggattacatt taatgagatg aaccatattg accctcaaac
    48481 agagcactcg gatattttta cttatatgat agcaggtatt aagtatagtg aaatagaaaa
    48541 taaggttcaa acattggcaa atgtagggta taatatgact ttggcgggtg taaaggttgc
    48601 aaagttggct agagaaatca atcccaacaa taaagttgga tgtgtatttg gactaaatcc
    48661 aatttatcca tacaattgta atcctagtaa tgtgttaaat gcatttttag ataatgatcg
    48721 tgattattat caaattgatg caatgtgcaa tgggaagttt ccccaatata aattaaaaga
    48781 atatgaaaaa aataatataa ctattgaagt gactgatgaa gacaaaaaag catttaaaga
    48841 aggtaagtta gattttatag gtcttaatta ttatttttca agtgtatcta ctccaaaaga
    48901 aaatttatca ggtgataaat ctttatttgg tgggattcaa aatccatatt tggaacaaag
    48961 taagtgggga tgggcaatag accctatagg tttaagattt accttgaact atatttacag
    49021 aaagtatcaa ttacctatct taattacaga aaatggttta ggggcatatg ataaaatcga
    49081 agaagatgga actataaatg atgattatag aattgaatat ttaagtaagc atctatctca
    49141 aatgaagtct gcaattgaag aggataatgt tgaatgtttt ggatatttga tgtggggacc
    49201 aattgattta gtcagtgcaa ctacaggtca gatgagtaag agatatggat ttatatatgt
    49261 tgacttagat gatgaaggca aaggaagttt taagagatat aagaaaaaat catttgattg
    49321 gtacaaagaa gtaataaaat ctaatggaga agttttagat ttttctacgg atatagtagt
    49381 agggtagtaa taagataaat agaagttagg aactaatgtt aattagtgtc tagtaaagca
    49441 tttaaaatag agtatagggt ctttaaagta aagtgtgtat tcttattaaa agtaatggca
    49501 actaaaatta ctataaaaag gagaatacac attttttatg tttatactta agtaaatatt
    49561 tagacatatt catagtataa tttactcatt atataaaaat agtatgattt actcactata
    49621 taactttata aattttctag aaaaatgtca gaatatattg acaaaaaaaa gatttgaaat
    49681 tataatctat attgagaata gtttgacaat ataaaaacaa gtatttttac aaatagtgaa
    49741 attattttta ttgtcaattt aagaataatt taggactact ataaagatga aggtatagat
    49801 gtcattatat gataaatcat acttgtatag aaagaatttt aaagtatgct tatcattgat
    49861 ttaatataat aaataatttt tgtttctagt aatagttcat aggaataaga tatctaaatt
    49921 cgcttagaaa tagtctaatt tacaaaggag gggatgcact agtagataaa atttttatat
    49981 aaagaagact taaagtggag tatattctga taatattggt ttaatggtta tattattatt
    50041 tcgttttaga tattttaaga ataatttaag acgtttgtaa aaatgtattc tatatctaat
    50101 atttaaataa atataagtct tccaacatgt atactaagat agaaagtagt tgaagtacaa
    50161 ttctaacttg taaaattatg tagaaagtaa aaatcaaaaa gaaatctagt aagttgataa
    50221 ttatagttaa tatttagaga gttaactcta gttatattta tttagatatt ttaggaataa
    50281 tttaagacgt atataatatc atcaaaaaat atcaagctat accatatgta gttaagaata
    50341 actgcataat atacatttta taattattgt tttattgata aaaaattatt atatttagga
    50401 ggaaacaaca atggaaaatt tttggaatcc acttacttgc tttactgtat gtgtaatgat
    50461 ttatgctttt agtgagtttt tatctaagaa aacaaaaggt gcaatttcaa cattgttatt
    50521 tgtatgtatt atatttttac taggattttg gacaaatgtt ttaccaaaag acattacagg
    50581 agcatcaggg atattagctg caatgagcaa ctttggtata gctttattaa taactaactt
    50641 aggaacactt ataaatcttg aagacttatg tcatgaatgg aaaacggttg taattgcatt
    50701 agtaggtatt gtaggaatag gggttggctg tttaacagtg ggtagtatgt tatttggtag
    50761 agagtatgct ttaatagcag cacctccaat agcaggttct actgttgccg gtattatcgt
    50821 aacaactgtt gcagaagctg caaatagacc tgaacttgct gcatttgtaa ttctagtatt
    50881 atcatttcaa aagttctttg gcattccgat aactacatat tgtataaata aagaattaaa
    50941 atttaaaaga gataacgggg attttttgaa agatgagtca aaaagtaatt ttagacttcc
    51001 aagtatgaga atttttaaag aagatttagg taaaaaatca tctactctag tgtatcttgc
    51061 aaagctttca tttgttgcta tgattggtaa ttttgtagga ttggctactt taataccagg
    51121 atttacccct gcaaactatt atttaaatcc taatatagca tgtttgttag tagggttgat
    51181 ttttacacga ataggttttt tagaaaaagg aattttagat aaatctcaat ctaatggaat
    51241 tataatgttt ggttgtatgt tgatgttacc tggaggactt gctaaggtaa ctccaacagc
    51301 attgtttggg atgattgttc caactatagg aatacttgtt gtaggttctg tatttataat
    51361 attatttact gcaattgtag gaaaactatt gggatattct ccaagaatgt cagctgcaat
    51421 tggtgtaact tgtatgctag cttatcctgc aacacagata atttccaacg aaggtattga
    51481 tgctatggaa ggaactgaag aagaaaaaca aagggcaaca gattatttat taccaaagat
    51541 gattatagga ggatttgtta ctgtaaccat tgcatcagtg gcatttgcta gtataatagg
    51601 tccaattata tttggttgat agtaaatata cttataggag gaataagaaa tgataggaag
    51661 tatatctata aatgagatag ataaaaaaag attcaaactt gatgatttaa gtaagaaaat
    51721 atgggaaaac ccagaaaaag catttaaaga atttaaagca tgtgaaaata cagcaaactt
    51781 tctaagaagt gaaggatttg atgtggaagt aggtgtagga ggtttagcca cggctattag
    51841 agctagtttt ggttcaggta aaccagttat aggttttatg ggagaatttg atgcattacc
    51901 tggtttaaat caaaaagttt caacaaagca agaagcattt gaattgagtg catatggtca
    51961 tggatgtggg cataatttat tatgtacagc acatgtaggg gcagtagtag gacttaaaag
    52021 agagatgcta gaaaataact taagtgggac tatagtattt tatgcatgtc caggagaaga
    52081 acaattaaca ggtaaaggat ttatggctag aggtggagca tttgaagggt tagacttagc
    52141 tataaatttt catccaaata aaattagtga agctacaatt ggtacttcaa cagctgttaa
    52201 ctctgtaaaa tttcacttta agggaaagac tgcacatgca ggctcagacc ctcaaaatgg
    52261 aagaagtgca ctagatgctg tggaacttac taatgtggga gctaattatt tgagagaaca
    52321 tgttacttct gatgtaagga ttcattatac aattacagat ggaggagtag cacccaacat
    52381 agttcctgat aaagcatcag tatggtatta tacaagagct ttaagtagag aagctgtaga
    52441 aagtacatat gaaagacttg taaaggtagc aaaaggagct gcaatgatga ctgaaactga
    52501 agttgaagta gagtttttag gaggttgtta taatacttta aataatcatg ttttagcaaa
    52561 tcttgtatca gaatgtatga atgagattaa acaagagcct tggactcgag aggaattgga
    52621 ttttgcagct gaattagata gacaaagccc acaacaatat aaggctatga taagtaaata
    52681 taatttgcca gaaggaactc atctatatta tggtggagga aatgttacaa actttaatag
    52741 ttatggttct actgatattg gagatgtaat gcatatagtt ccgacagcat acttttttac
    52801 agggtgtacg aatctaggtg caccaggtca tagctggcag tttacatctt gtgcaggaag
    52861 ttctatagga gaaaaaggta tgatatatgc atcaaaaatc atggctatgt ttggagccaa
    52921 aatattaaat aatccagaga tagctaaaaa ggcaaaagaa gagtttgata aatctatgaa
    52981 tggtaaaaca tataaatgtc ctattccaga tgatattcca actccttaaa aagacatgaa
    53041 gtttaatata taaaattata atttttatat cagcatcatt tcattactaa aagaaagtga
    53101 gattttgctg actatctgat taggaggaag gaatatgaaa aattttttta aagaagcgca
    53161 gtcaatagaa ggggaaatta ttagatggag aagatattta catgaaaatc ccgaaattgg
    53221 ttgcaatcta ccgaaaacag taacctttgt taccagtact ttagaaaaaa tgggatattc
    53281 tccgaaattg acgtctaata atggaatagt agctgtttta gacagtgaaa atggtggaaa
    53341 gaccttatta ttaagggcag atatggatgc acttcctatt atggaggaga cggatttacc
    53401 ttttaaaagt aagaataaag atgcatctca tagttgtgga cacgataatc acacagcaat
    53461 gctcttagga gcagctaaaa tacttatgga taataagcac ttacttaaag gtagagtaaa
    53521 gtttgtattt gaaccagatg aagagagtac cagaggtgca aaatcaatga ttgatgatgg
    53581 tatattagaa aatcctcagg tcgatgcagc tatggcattt cacagtatgg tgggtaaatt
    53641 tcttaacaca ggtcaagtag cttattcacg tggtccagct atggcatctg cggatatatt
    53701 tgaaataaca gtagaaggaa aagcaactca tggagcaagc cctgaatttg gagtggaccc
    53761 aataaacata atgtcgcata tacaaatagc attaaacact atactttcaa gagaaaaacc
    53821 tcagaaagag cctgtagttt taactattgg tatgtttaat gcaggaaacg taccaaatgc
    53881 tataccaaat aaagcatcta tgtcaggaac tttaagaaca tttgaccaat ttgttagaga
    53941 aaaattaaaa aatagaatta ttgaaatatc tacaggaata gctaaatcat ttggaggaaa
    54001 tgctaaagtg gaatttaaaa tggggactcc cgcagttata aatgatttgg atgtaggaga
    54061 tgagattgta tcttatgtta aagaaattat aggaaatgag aatgtaatag actttccatc
    54121 tgcaatgggt tcagaagatt tttcagagat tttactaagg gttccaggag tatttttatg
    54181 gattggaatg ggaagtatta gtcagggata taattttggt atgcataatc ctaaagcaat
    54241 ttttaatgaa gagggattag tttatggttc agctttattt gctcattgtg cagtaaaatg
    54301 gttagaaaat aattttagta gataataaca taaaatatta ttaaatatca taaaaggtat
    54361 atcttaaaaa gatgcaaaga tatacctttt agaaagtcat atataatttt acttaatatt
    54421 agatgataaa atatttttat gcattttgga ttttaatatt tattttctga tataaataat
    54481 ttgtataccc tttcaggtct accttttgtt gagttttgtt tttcatataa tatagtagcg
    54541 tttccagact ttaagagact acttagaaat ctattagcag ttctaactgt tacatttaaa
    54601 catgaagata attccttagg agtaacttca tctctttgta gaatttcagt aacagatata
    54661 atcttttgaa tagtaagagt agacaattta gaacttttgg caattgtatt tatatgtgga
    54721 gttactttat ttgaaacagt aagtaactta tcacacagta aaggtcctat tgtctcataa
    54781 ttttcgttga ctaagaaact gtaatttaaa ggatttattt tagattcctt attagcactt
    54841 atggcactag acatagcttg ctttatatca ttaccaattc catatccaac acatacttta
    54901 atatttgtac tattttcaat agattctttt atataacatg cagtaaaatt tttagttaat
    54961 gatttaatcg tttcacattt agtgaaaatt ttaaaagaat tactattttt ttcaattata
    55021 aaatcggtta atttatcttt tttaatacta tttaatattt tataaacttg gtctagagta
    55081 gagtcatcat aatttgtatc tttaactgtt atatatatta cacttggttg atttcccttc
    55141 attctatgta aatgaatatc ttttattaag ttattaaaag tattttttaa taaatctata
    55201 tcagggtata tgaaatgaaa gtttactcct gcattttgta acataggaac aatactacta
    55261 aaacgagtag tagaaaaatc gattttccct tcattccata gattaatatg tttttttgca
    55321 atcttttttt caagttttgc tatgtcagat aaactcattt tagatgcatt ttcatcaata
    55381 ctatccatta agtctccaaa tgtgccattt gataaataat atgatatatc ttttggatta
    55441 tctgcccagt ctgttaagtc aaagtatgct ctagaaaaat ctaatgtatt atatttaaag
    55501 ataagcttta gaaacatatc atagtaactt tgtaagtcag taccaaactg aacctcccat
    55561 gactaaagtc acagggttcc tgcttcatag aattttgcat actaaaatat gtcttactaa
    55621 ttcttcacag gccatccccg tagttcctac ggttcttaca taagttattt attttgaatt
    55681 gttattagtc ttaatccttc ttttaatata tttatacttg catttatatc tctatcgtga
    55741 gtttcattac aactaggaca aatccattct cttatattta agtccttcac ttcctcgttt
    55801 ttatacccac atttattgca tatttgtgaa cttgcaaaga atttacctac ttttacaatt
    55861 tttctaccat gccaattagc cttgtattct aattgacgaa taaactctga ccatgataca
    55921 tcagaaatta agcgagatag tctacgattt ttaatcatat tctttacttg taaatcttct
    55981 atgcaaataa tatcattctc ttttattaat ttagtagata atttttgtag aaaatcattt
    56041 ctttgattag ctatatgttc ttgaagtct
//
LOCUS       DAMKPG010000013        55937 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMKPG010000013 DAMKPG010000000
VERSION     DAMKPG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.015"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="PC621_000862"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="PC621_000862"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..276
                     /locus_tag="PC621_000863"
     tRNA            202..276
                     /locus_tag="PC621_000863"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Asn,seq:gtt)
     gene            282..367
                     /locus_tag="PC621_000864"
     tRNA            282..367
                     /locus_tag="PC621_000864"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Leu,seq:taa)
     gene            383..458
                     /locus_tag="PC621_000865"
     tRNA            383..458
                     /locus_tag="PC621_000865"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Met,seq:cat)
     gene            466..540
                     /locus_tag="PC621_000866"
     tRNA            466..540
                     /locus_tag="PC621_000866"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            555..628
                     /locus_tag="PC621_000867"
     tRNA            555..628
                     /locus_tag="PC621_000867"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Gly,seq:tcc)
     gene            634..709
                     /locus_tag="PC621_000868"
     tRNA            634..709
                     /locus_tag="PC621_000868"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Val,seq:tac)
     gene            715..791
                     /locus_tag="PC621_000869"
     tRNA            715..791
                     /locus_tag="PC621_000869"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Asp,seq:gtc)
     gene            802..876
                     /locus_tag="PC621_000870"
     tRNA            802..876
                     /locus_tag="PC621_000870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Gly,seq:gcc)
     gene            886..959
                     /locus_tag="PC621_000871"
     tRNA            886..959
                     /locus_tag="PC621_000871"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Arg,seq:tct)
     gene            1935..2774
                     /locus_tag="PC621_000872"
     CDS             1935..2774
                     /locus_tag="PC621_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF4108045.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2771..3952
                     /locus_tag="PC621_000873"
     CDS             2771..3952
                     /locus_tag="PC621_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108046.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4256..5158
                     /gene="ptb"
                     /locus_tag="PC621_000874"
     CDS             4256..5158
                     /gene="ptb"
                     /locus_tag="PC621_000874"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF4108047.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5180..6259
                     /gene="buk"
                     /locus_tag="PC621_000875"
     CDS             5180..6259
                     /gene="buk"
                     /locus_tag="PC621_000875"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF4108048.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6249..6926
                     /locus_tag="PC621_000876"
     CDS             6249..6926
                     /locus_tag="PC621_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108049.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6969..7184
                     /locus_tag="PC621_000877"
     CDS             6969..7184
                     /locus_tag="PC621_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4108050.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7213..8292
                     /locus_tag="PC621_000878"
     CDS             7213..8292
                     /locus_tag="PC621_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF4108051.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8292..9044
                     /locus_tag="PC621_000879"
     CDS             8292..9044
                     /locus_tag="PC621_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF4108052.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9045..9602
                     /locus_tag="PC621_000880"
     CDS             9045..9602
                     /locus_tag="PC621_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF4108053.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9772..11118
                     /locus_tag="PC621_000881"
     CDS             9772..11118
                     /locus_tag="PC621_000881"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF4108054.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11385..13217
                     /gene="glmS"
                     /locus_tag="PC621_000882"
     CDS             11385..13217
                     /gene="glmS"
                     /locus_tag="PC621_000882"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF4108055.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13670..14965
                     /locus_tag="PC621_000883"
     CDS             13670..14965
                     /locus_tag="PC621_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF4108056.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15442..16179
                     /locus_tag="PC621_000884"
     CDS             15442..16179
                     /locus_tag="PC621_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF4108057.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            16200..17453
                     /gene="murA"
                     /locus_tag="PC621_000885"
     CDS             16200..17453
                     /gene="murA"
                     /locus_tag="PC621_000885"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF4108058.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17540..18604
                     /gene="spoIID"
                     /locus_tag="PC621_000886"
     CDS             17540..18604
                     /gene="spoIID"
                     /locus_tag="PC621_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF4108059.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18670..19338
                     /locus_tag="PC621_000887"
     CDS             18670..19338
                     /locus_tag="PC621_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF4108060.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19436..19696
                     /gene="spoIIID"
                     /locus_tag="PC621_000888"
     CDS             19436..19696
                     /gene="spoIIID"
                     /locus_tag="PC621_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF4108061.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19893..20912
                     /locus_tag="PC621_000889"
     CDS             19893..20912
                     /locus_tag="PC621_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF4108062.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20928..21356
                     /gene="fabZ"
                     /locus_tag="PC621_000890"
     CDS             20928..21356
                     /gene="fabZ"
                     /locus_tag="PC621_000890"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF4108063.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21467..21976)
                     /gene="yyaC"
                     /locus_tag="PC621_000891"
     CDS             complement(21467..21976)
                     /gene="yyaC"
                     /locus_tag="PC621_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF4108064.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22165..22268
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22405..23598
                     /locus_tag="PC621_000892"
     CDS             22405..23598
                     /locus_tag="PC621_000892"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF4108065.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23914..25011
                     /locus_tag="PC621_000893"
     CDS             23914..25011
                     /locus_tag="PC621_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108066.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25275..27494
                     /locus_tag="PC621_000894"
     CDS             25275..27494
                     /locus_tag="PC621_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF4108067.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27497..28279
                     /locus_tag="PC621_000895"
     CDS             27497..28279
                     /locus_tag="PC621_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF4108068.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28611..30524
                     /locus_tag="PC621_000896"
     CDS             28611..30524
                     /locus_tag="PC621_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4108069.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30665..30973
                     /locus_tag="PC621_000897"
     CDS             30665..30973
                     /locus_tag="PC621_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4108070.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30998..31324
                     /locus_tag="PC621_000898"
     CDS             30998..31324
                     /locus_tag="PC621_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108071.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31363..32697
                     /gene="celB"
                     /locus_tag="PC621_000899"
     CDS             31363..32697
                     /gene="celB"
                     /locus_tag="PC621_000899"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF4108072.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            32708..33226
                     /locus_tag="PC621_000900"
     CDS             32708..33226
                     /locus_tag="PC621_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF4108073.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33322..34281
                     /locus_tag="PC621_000901"
     CDS             33322..34281
                     /locus_tag="PC621_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF4108074.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34329..35381
                     /locus_tag="PC621_000902"
     CDS             34329..35381
                     /locus_tag="PC621_000902"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF4108075.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35384..36058
                     /locus_tag="PC621_000903"
     CDS             35384..36058
                     /locus_tag="PC621_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF4108076.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36611..37162
                     /gene="raiA"
                     /locus_tag="PC621_000904"
     CDS             36611..37162
                     /gene="raiA"
                     /locus_tag="PC621_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF4108077.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37294..39969
                     /gene="secA"
                     /locus_tag="PC621_000905"
     CDS             37294..39969
                     /gene="secA"
                     /locus_tag="PC621_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF4108078.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39984..41091
                     /gene="prfB"
                     /locus_tag="PC621_000906"
     CDS             join(39984..40058,40060..41091)
                     /gene="prfB"
                     /locus_tag="PC621_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF4108079.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41235..43376
                     /locus_tag="PC621_000907"
     CDS             41235..43376
                     /locus_tag="PC621_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF4108080.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43524..44690
                     /locus_tag="PC621_000908"
     CDS             43524..44690
                     /locus_tag="PC621_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108081.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44750..44867
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44992..45597
                     /locus_tag="PC621_000909"
     CDS             44992..45597
                     /locus_tag="PC621_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF4108082.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            45926..46966
                     /locus_tag="PC621_000910"
     CDS             45926..46966
                     /locus_tag="PC621_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF4108083.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            47105..47557
                     /gene="tsaE"
                     /locus_tag="PC621_000911"
     CDS             47105..47557
                     /gene="tsaE"
                     /locus_tag="PC621_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF4108084.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            47557..48273
                     /gene="tsaB"
                     /locus_tag="PC621_000912"
     CDS             47557..48273
                     /gene="tsaB"
                     /locus_tag="PC621_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF4108085.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            48263..48739
                     /gene="rimI"
                     /locus_tag="PC621_000913"
     CDS             48263..48739
                     /gene="rimI"
                     /locus_tag="PC621_000913"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF4108086.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            48745..49761
                     /gene="tsaD"
                     /locus_tag="PC621_000914"
     CDS             48745..49761
                     /gene="tsaD"
                     /locus_tag="PC621_000914"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF4108087.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            50058..52766
                     /gene="hpdB"
                     /locus_tag="PC621_000915"
     CDS             50058..52766
                     /gene="hpdB"
                     /locus_tag="PC621_000915"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF4108088.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            52770..53027
                     /gene="hpdC"
                     /locus_tag="PC621_000916"
     CDS             52770..53027
                     /gene="hpdC"
                     /locus_tag="PC621_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF4108089.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            53020..53970
                     /gene="hpdA"
                     /locus_tag="PC621_000917"
     CDS             53020..53970
                     /gene="hpdA"
                     /locus_tag="PC621_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF4108090.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            54290..55252
                     /locus_tag="PC621_000918"
     CDS             54290..55252
                     /locus_tag="PC621_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4108091.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(55351..>55937)
                     /locus_tag="PC621_000919"
     CDS             complement(55351..>55937)
                     /locus_tag="PC621_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4108092.1"
                     /translation="SNKKNLYPVKNIKLGSLLIIYFMISVIVPTSFILILQGAQPNYS
                     GIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVIILGMCWSLWHLPL
                     IFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCAIIHVLNNSAFLITNSK
                     ESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT        22395 a         6054 c        10636 g        16852 t
ORIGIN      
        1 taactctaaa gaaactagta ttgctaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgttaaatg ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
      241 ggtaggctgt gggttcgagc cccacccttg gagccaaaaa agccgaagtg gcggaactgg
      301 cagacgcaca ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc
      361 ttcggcactt ataaaacaat atcgcggggt ggagcagttg gcagctcgtc gggctcataa
      421 cccgaaggtc gtaggttcaa gtcctgcctc cgcaaccatt aaaacggctc gttggtcaag
      481 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      541 attcaattaa atatgcgggt gtagctcaat ggtagagttc tggccttcca agccagctgt
      601 gagggttcga tccccttcac ccgctccaaa aatgggacca tagctcagct gggagagcac
      661 ctgccttaca agcagggggt cacaggttcg agccctgttg gtcccaccat tcgcggcctg
      721 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgagggtt cgagcccctt
      781 ccaggtcgcc aaattaaata agcgggaata gttcagtggt agagcgcaac cttgccaagg
      841 ttgaagtcgc gagttcgaat ctcgtttccc gctccataat aacttgggtg catagctcag
      901 ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcat
      961 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
     1021 catgtatatt acctgtgtct ttttatttat aaactttaaa ctatgtaaaa attacagtat
     1081 attctaatag agttacagta tattcattaa tattgtaaac atacggaggg taatgtataa
     1141 tagtctacaa gatgtttatg aaggcatcag ttaaatataa ctaattagat aagagttata
     1201 tacattatat tgtatacttt atggtattta tatattgaaa taacttatga aattatttat
     1261 aaagagattt atgagcatat ttttgtctaa acaataagta taagaaatta taaaaaatta
     1321 gagactatag tgaattgaca atttaatgta atatttataa gttatactaa gtaaattatt
     1381 atactgtgaa atggatatat aactttaggt attgtgtatt attttcactg taggagatta
     1441 tatctagtta tacataataa gttttaatga tagtttatag taagatatta aaataaattt
     1501 atctaatcta gatatgaaat aagttattac tttaatataa aaatgaatct aatggaataa
     1561 tattgatgat aagtaatatt tagattttgt atttgtttat agttaataag tggattatag
     1621 aatttataat aattaatata tattaaacaa agatatatta agatatttta gaacataaaa
     1681 ctgcattaag gcgcgagtac tattttgtat tcgcgccttg atggtttgag ttatttgagt
     1741 aaaaactaac tttaataaga aatatgtatt tattaagttg atatttttaa tgtgtttaga
     1801 gattatttaa caatctccaa atataaaact agaattattt acataaaagt taaatagttt
     1861 gtatagagtt agatttatat agtataatac ttagtgtaaa taattaagaa gttatcaaac
     1921 aggaggataa aactgtgtta gatataaatt cttttttaaa tggatttttt aatataatat
     1981 taagaatgag tatatatgat ttaattgaca tatctatagt agcgtatatt ttttacaaaa
     2041 tatttatgtt tataaaagat acaagagcag aacaagtgtt taaaggtata atatttcttt
     2101 tattagcaac tcaacttagt aacaccttta aattgcatac cgtttattgg atatctctta
     2161 aagctttaga ttatggagtt atagctgctc ttataatttt tcaaccagaa tttagagcag
     2221 gacttgaaca tataggaaga gcaaagttta atttatttgg aaagaatgta aatacttctg
     2281 aagaaactct caatagaaat atagaagaaa ttgtagaagc tttatattca ctttctagac
     2341 aaaaaatagg agctcttatc ataatggaga gagaaactag aataagtgat ataataaata
     2401 caggaactat tattgatgct gaaatttcta ggcaactatt aataaatata tttataccaa
     2461 atacaccatt acacgatgga gctgttgtaa ttagagattc taaggtaaaa gcagctgcat
     2521 gttttttgcc attgacggaa agtaaggatt taagtaaaga tttgggtact agacatagag
     2581 caggaatagg ggtaagtgag gtgtctgact gtataactct gattgtatca gaggaaacag
     2641 gtggcgtatc aattgctaaa gcaggtaagt tatatagaga tatttctaga gaaagaatga
     2701 tgaatatatt acgtagtaat ttaaaaacaa atacagaaac aagaagtttt ttcaaaggtg
     2761 gtatatttaa atgataaata gactaaaaaa taataccaaa ataaaaataa tatcattttt
     2821 aagtgcaatt gcattatgga tgtatgttat ggctgtagtt gaccctgaag aaactaaagg
     2881 gtttgaagat atacctgtat ctataagtaa tatgagtgat ttaaaggata aaaatcttgt
     2941 tatatatcca gacgaaaaat taacagcaga tgtatatata aaaggtagtt tatcaaaggt
     3001 acaaagtgtt aagaaagaag atatacatgt atatggaacc attgaagacc caatacaagg
     3061 acagaaggaa gtatatttaa aagcaaatat tcctcaaggt gttactctag aatttaagca
     3121 tgatgtttta gtagtaaatc ttgaaaaaaa cataaaagaa aaaagagata taaaggtaga
     3181 agtaagtgga aattcaaaaa gaaatataaa taaaattgaa cttagtaagg aatttgtcga
     3241 tgtatcaggt ccaagaactc tagttaaaga agtcaaaagt gtaagagcta atctagatgt
     3301 tggtaatgag acagatgatt tttctaaaaa attgcaatta ataccattaa atagtgatgg
     3361 aactaaggtt gaaggagtaa cattaagttc atcctttgtg actgcaaaga tagaactatt
     3421 gaaggaaaaa attgtcccaa taagagtaag tctaaatgat aataaagata atgatgtact
     3481 gaaaaactat aaattaagta aggaacaagt tacaataaaa ggaaagaaag aagatataga
     3541 caaaataagt tatattaaga caaaacctat tgacttagct gatttggcta atggtgaaga
     3601 aaaagaagtt agcttagaaa ttccagaggg tataactctt gaggatagct ctataacaat
     3661 taaattaaat gatacaaaac aattgagtgc agaattttta tataatgcgg gagatattga
     3721 attaagaaat atcccaacaa atatagataa tccaaatgta atttctgatg tagatataaa
     3781 agttgttgta gagtcaacgg aggatttgag taagttaaat aaaaaagata tttcattata
     3841 tattgattta tcacaaggcc ctgaggaaga tagtaaatat aaaataaaat atgaaacaac
     3901 atatcaattt aaaaaaataa atatagaacc taatattgtt gaagtggaat gaacaaatat
     3961 ttcgaatatt tagttgttac aagatgcgac aaaaaaacct ataaaattaa gtttgttaat
     4021 tttatgtttt tgttgcatct tgtttatttt agttatatta taaaataagg acaatgagat
     4081 atttgttaaa tctaaaaagc gctagatata tgaaaaaaga ctgataaata gatttaaata
     4141 aatattttga attaaatatt tcaattatct ctaggaaaac gttgctgaac gagttataat
     4201 ttaattgaga tataaaaagt tctatttcaa aaaattcaag atgaggtgga aatgcatgag
     4261 aagttttgaa gaagtaatta agtttgcaaa agaaagagga cctaaaacta tatcagtagc
     4321 atgttgccaa gataaagaag ttttaatggc agttgaaatg gctagaaaag aaaaaatagc
     4381 aaatgccatt ttagtaggag atatagaaaa gactaaagaa attgcaaaaa gcatagacat
     4441 ggatatcgaa aattatgaac tgatagatat aaaagattta gcagaagcat ctctaaaatc
     4501 tgttgaatta gtttcacaag gaaaagccga catggtaatg aaaggcttag tagacacatc
     4561 aataatacta aaagcagttt taaataaaga agtaggtctt agaactggaa atgtattaag
     4621 tcacgtagca gtatttgatg tagagggata tgatagatta tttttcgtaa ctgacgcagc
     4681 tatgaactta gctcctgata caaatactaa aaagcaaatc atagaaaatg cttgcacagt
     4741 agcacattca ttagatataa gtgaaccaaa agttgctgca atatgcgcaa aagaaaaagt
     4801 aaatccaaaa atgaaagata cagttgaagc taaagaacta gaagaaatgt atgaaagagg
     4861 agaaatcaaa ggttgtatgg ttggtggacc ttttgcaatt gataatgcag tatctttaga
     4921 agcagctaaa cataaaggta taaatcatcc tgtagcagga agagctgata tattattagc
     4981 cccagatatt gaaggtggta acatattata taaagcttta gtattcttct caaaatcaaa
     5041 aaatgcagga gttatagttg gggctaaagc accaataata ttaacttcta gagcagacag
     5101 tgaagaaact aaactaaact caatagcttt aggtgtttta atggcagcaa aggcataatt
     5161 ttaaggggga aataggcaaa tgagcaaaat atttaaaatc ttaacaataa atcctggttc
     5221 gacatcaact aaaatagctg tatttgataa tgaggattta gtatttgaaa aaactttaag
     5281 acattcttca gaagaaatag gaaaatatga gaaggtgtct gaccaatttg aatttcgtaa
     5341 acaagtaata gaagaagctc taaaagaagg tggagtaaaa acatctgaat tagatgctgt
     5401 agtaggtaga ggaggacttc ttaaacctat aaaaggtggt acttattcag taagtgctgc
     5461 tatgattgaa gatttaaaag tgggagtttt aggagaacac gcttcaaatc taggtggaat
     5521 aatagcaaaa caaataggtg aagaagtaaa tgttccttca tacatagtag accctgttgt
     5581 tgtagatgaa ttagaagatg ttgctagaat ttctggtatg cctgaaataa gtagagcaag
     5641 tgtagtacat gctttaaatc aaaaggcaat agcaagaaga tatgctagag aaataaacaa
     5701 gaaatatgaa gatataaatc ttatagttgc acacatgggt ggaggagttt ctgttggagc
     5761 tcataaaaat ggtaaaatag tagatgttgc aaacgcatta gatggagaag gacctttctc
     5821 tccagaaaga agtggtggac taccagtagg tgcattagta aaaatgtgct ttagtggaaa
     5881 atatactcaa gatgaaatta aaaagaaaat aaaaggtaat ggcggattag ttgcatactt
     5941 aaacactaat gatgctagag aagttgaaga aagaattgaa gctggcgatg aaaaagctaa
     6001 attagtatat gaagctatgg catatcaaat ctctaaagaa ataggagcta gtgctgcagt
     6061 tcttaaggga gatgtaaaag caatattatt aactggtgga atcgcatatt caaaaatgtt
     6121 tacagaaatg attgcagata gagttaaatt tatagcagat gtaaaagttt atccaggtga
     6181 agatgaaatg attgcattag ctcaaggtgg acttagagtt ttaactggtg aagaagaggc
     6241 tcaagtttat gataactaat gataaaagaa taagaatcat aactggtcac tatggaagtg
     6301 gtaaaagtga atttgcgatg aactatgttg ttaagttaag agatatggtt tctggcaaag
     6361 ttgctatagc cgatttagat gtggtaaatg tatactttag aactagagaa aagaaagagt
     6421 taatgaaatc cttaggtata caaccaatag atagctcaat taatgctcca acattagatt
     6481 taccagcagt atcagcagag gtaatgtctc ctatggtaga ccattcatac aattctgtta
     6541 tagatttagg tggagataat gtaggtgcta gggttatagg aagatttagt catttgttaa
     6601 aagaaggcga ttatgatatg ctttttgtaa taaatgcaaa tagggagaaa actcaaactt
     6661 ctgaagaagt tatccaatat ataaaagaaa tagaaaaatc ttctaaatta aaggtaacag
     6721 gtctaattaa taatactcat cttattaggt ttactactat tgatgatgtt ttaagaggtc
     6781 agaaagttgc aaaagaagtt tctgaaaaat gtaatatacc gataagatat gttgcatgtt
     6841 tagaaaatct tgttgaacag ttaccaaaag atttagaagg agaaattttt cctataaaat
     6901 tatatatgag agaagactgg atgtagtttg agctcaaata tatagaaagt ttaaggaggc
     6961 ttaggtaaat ggctaaagga aaagtatctt ttaatcaaga acgttgtaaa ggttgtggat
     7021 tgtgtgttga agcatgtcca gtaaaaatca tacaattaga ctcaaatgta ataaacaaaa
     7081 aagggtataa cccagcaact gtttttgaaa tggaaaaatg cataggatgt gcaagttgtg
     7141 caactatgtg tccagatgta gttataacag tagaaagaga ttaatttaat taatatttaa
     7201 gggggaattt taatggctaa gatacttatg aaaggtaacg aagcatttgg aaaagcagct
     7261 atagaagctg gttgcaaata tttcttcggt tacccaataa ctccacaaag tgaattacca
     7321 gaatatttat caagagagtt acctaaaata ggtggagcgt tcgttcaagc tgaatcagaa
     7381 gtttctgcga taaacatggt ttatggtgga gcaggtgcag gagcaagagt tatgacttct
     7441 tcatcttcac caggagttgc tttaaagcaa gaaggtataa catatgcagt aggagcagaa
     7501 gttccttgtg ttgtacttaa tgtaatgaga ggtggtccag gacttggaag tatacaacct
     7561 tcacaagctg actactttat gtctacaaga ggtggaggaa atggagacta cagaactcca
     7621 gtattcgcac cagctacagt tcaagaagct gttgatatga taatggaagc ttttgacgtt
     7681 gcagattatt acagatcacc agttatggta gttgcagatg gtatgatagg tcaaatgatg
     7741 gagccagttg aatttagagc tcctgagaag aaaagagaat tgcctcctaa agattgggca
     7801 actgttggaa ctaaaggaaa aagaaaacct aatgtaataa actctctata cttagaacca
     7861 gaagtactag aagaccattg ttggcattta caagaaaaat ttgatgcaat ggaaaagaat
     7921 gaagtacaat acgaaatgta caaaacagaa gatgctgaat ttgtatttgc agcttatgga
     7981 actacttcaa gagtagtaaa aagcgcaatt gatatattaa gagaagaagg aataaaagca
     8041 ggtcttataa gacctaaagt attatggcca ttcccattcg aagcattcaa ccaaattcct
     8101 aatgctagaa acatattgac tgttgaaatg agtatgggac aaatggtgga agatgttaaa
     8161 atggcagtag aaggtaaatt accagtttac ttccatggaa gaccaggagg aatgactcca
     8221 acaccagcag aaatagttga aaaagctaaa aaaattattg ccggagaatt ggttgcagga
     8281 ggtgctagat aatggctgtt gtatttaaga aaactgaagg attacaagat actcaaactc
     8341 actactgtcc aggatgtaca catggtataa tccatagatt agttggtgaa gtattagaag
     8401 aactaggagt attaggtgat gctgtaggtg ttgttccagt tggatgttca gttttaggat
     8461 ataaatattt taactgtgac actcaagaag ctgctcatgg tagagcgcca gctgctgcta
     8521 caggaataaa aagagttcat cctgaaaata cagtattcac ttatcaaggt gatggagact
     8581 tagcttcaat tggtactgct gaaatagttc atgcagctgc tagaggagaa aaaataacta
     8641 ctatatttgt aaacaataca acttatggta tgactggtgg acaaatggct ccaactacat
     8701 tagttggaca aagagctact actgctcaat caggaagaaa tgctgaaact caaggttatc
     8761 caataagagt aagtgaaatg ttagctacat taacaggagc tgtatttgta gaaagagtag
     8821 cagttgatac tccagctcat gttagacaag ctaaaaaagc tataaagaaa gcattccaag
     8881 ttcaacaagc aggattagga tttggaatag tagaagtatt atctacttgt cctacaaact
     8941 ggggactagc tccaaacgat gctttacaat ggttaagaga caacatgata ccatactatc
     9001 cattaggaaa tttcaaaaat gttgaagttg aggaggtgaa gtaaatgtct acagctagag
     9061 taatatgtgc aggcttcgga ggccaaggtg ttatgtctat gggacaatta cttacttatg
     9121 caggaatgct agaaggaaaa gaagtttctt ggttaccatc ttatggacca gaaatgcgtg
     9181 gaggtacagc aaactgtgca gtaactgtat ctaatgagcc agttggttct ccacttataa
     9241 cagatgatgc aactgcagct atattattaa atataccagc atttgaaaaa ttcaaagatg
     9301 atgttattcc tggtggaaaa ataatagtaa atagttcttt aataaaagaa aaagttgata
     9361 gaacagatgt agatgtttat tatataccag caaatgaatt agcagctgaa ttaggaaatg
     9421 ataaagttgc taatatgata atgttaggcg cttacttaaa agtatctgat actgtagata
     9481 tagaatctgt actagaagca ttcaaaaaag ttttcggacc tagaaaagaa aaattcgttc
     9541 cattaaacag agaagcttta caaaaaggta tggatgctac atgttctaaa gcaactaact
     9601 aataaagaaa aagatgtcaa aaaaaagaca atagaattat ttctattgtc ttttttgctg
     9661 ataagtatta aaatgttttt atgactgaaa ttaagcctat aatgatattt tatgaactat
     9721 aaaaatataa tacaaattac aagcttttca attatgaaag gagaatcttt agtgagaaag
     9781 tattttggaa cagatggagt tagaggagta gcaaatacag aactaacatg tgatttagca
     9841 tataaacttg gtagagctgg tggatttgtc ttagctcaag gagaccacag agtaaaagta
     9901 gtagtaggta aggatactag aatatcaggt gatatgttgg aagcatcttt aatagcagga
     9961 cttatgtctg ttggatgtga tgttattact gttggaataa taccaacacc tgcagtggca
    10021 tatttaacta gaaaatatgg tgctgattgt ggtgtggtta tatctgcatc tcacaatcct
    10081 gttgaatata atggaatcaa attctttaat aagaatggat ataaattaga tgatgaaatt
    10141 gaattgaaaa tagaagaata tatagacgat atagacaaaa ttgattgtct tccaattggt
    10201 gaaaatgtag gaagaaaatt acatgaacat tgtgcacaaa gagactatgt agactaccta
    10261 aaatcaataa taagcactga ctttaaggga ttaaaagtag ttttggactg tgctaatggt
    10321 gcgtcatata aagtggcacc aatagtgttt gatgaactag gagcaagtgt aatttcaata
    10381 aatagttcac cagatggaaa taatataaac tataaatgtg gctctacaca tccagaacaa
    10441 cttcaaaggg ctgttttgga acataatgct gacttaggtc ttgcatatga tggagatgct
    10501 gatagattaa ttgcagtaaa tgaaaagggt caaattgttg atggagacca tataatgata
    10561 ttgagtgcct taaatttaaa gaaaaataat aaattggcgc aagatacttt ggtagttaca
    10621 gttatgagta atatagggtt gacaatagct gctaaggaaa atggaataaa tttatctact
    10681 acagcagttg gagacagata tgttttagaa gatatggtta aaaatggata taatcttggt
    10741 ggagagcaat ctggacatat gatattttta gattacaaca ctactggaga tggagtgctt
    10801 agttctctaa tacttgcaaa tataatcttg caagaaaaga aacctttatc agaaattgca
    10861 tcaataatga gccaataccc acaagtatta gtaaatgcaa caataaaaaa tgaaaataag
    10921 aacaaatata tggaatatcc agaaataaaa actgagatag aaagaataga atctatttta
    10981 aacggaaatg gtagagtttt gataagacca tcaggaactg agccattagt tagagttatg
    11041 ttagagggta aagaggaagg tcaaataaaa gaattagcaa caaacttagc taatcttata
    11101 caagaaaaat tatcgtaatt taaagggtat tctcagatac aaatactgag gacacctttc
    11161 taagcgccag aacttagtta tagacataat tattgagtaa tataggaact aagttgacga
    11221 ggtcagagtt aatcgaattg tcggcggatg ctctgcggtg tgtttaccat cgaaagactt
    11281 ctttaaataa taggagcaat cctatggaca aagggaagcc aaagtaaact tatatgacct
    11341 aactcttaaa atatataaat aatattttta ggaggacaca aataatgtgt ggaatagttg
    11401 gatatttagg aagtagaaag gcagcagaag ttatagtaga agggctttct aagctagagt
    11461 acagaggtta tgactcagca ggagtagcag ttaatagttc taatgaagaa gaacttaata
    11521 ttagaaagtt taaaggtaga ctttctgtac ttgcagaaga cttagaaaag aatcctattg
    11581 atggaaattt aggtataggg catacgagat gggcaactca tggagaacca tctgatgtaa
    11641 actcacatcc tcattttaat caagccaaaa caatagcagt tgttcataat gggataatag
    11701 aaaactatat ggaaataaaa gaagaattaa tttctgaagg tgttaagttt gaatctcaaa
    11761 cagatacaga ggtaatagca catttagtag ataaatacta tgagggtaat ttattagatg
    11821 ctgtatacaa aactatatca aaattaagag gagcttatgc attaggtgtt atatgtaaag
    11881 aacatggaaa tgaattagtt gcagtaagaa aagatagtcc tcttgtagtt ggtgttggtg
    11941 aaggtgaaaa ctttatagca tcagatatac ctgcattatt aaaatatact agagatgttt
    12001 atttcttaga aaatggagaa gtagttcatc taaaagacga aaatgtaaca gtttatgact
    12061 ctaatagaaa tcttgttgaa aaagaagtat ttcatgtaac ttgggatgta gaagctgcgt
    12121 ctaaaggtgg atatgactac tttatgtcaa aagaaattca tgagcaacca acaggagtta
    12181 gagaaacttt agaaagaaga cttgatgata atggaaatat aattctagac agtataaata
    12241 tttctaaaga agacttagaa aaaataaata aagtttatat agtggcttgt ggaacagcat
    12301 ataatgctgg attacttggt aagtatgcca tagaaaaatt tgtaaatata ccagtaataa
    12361 ctgatatagc atcagaattt agatatagcg acccatttgt agatgaaaat tcattagtaa
    12421 tattagttag ccaatctggt gagacagcag atactctagc agtattaaga gattcaaagg
    12481 ctaaaggagc aagaatactt tcaataacta acgttgtagg ttcatctata gcaagagaat
    12541 cagatgatgt attctatact tgggcaggac ctgaagtagc agttgcatca actaaggcat
    12601 atactactca aataacttct ttatacatga tagctttaga ctttgctatc aaaaaaggaa
    12661 caataactag agaattttat gatagtatga tttctaagat gaaagaaata ccatcaaaaa
    12721 tacaagaaat attagacaat gaagaatata taaaagaagt tgctaaaaca gttgttagtt
    12781 ctgaacatgc attctattta ggaagaggta tagactatag cttagcaatg gaaggttcat
    12841 tgaaattaaa agagatttca tatatacatg cagaagcttt tgcagcagga gagctaaagc
    12901 atggaacaat agctttaata gaaaaaggaa ctcctgtaat tgccatagct actcaagaaa
    12961 agttatttga aaaaatggtt tctaatatgg aagaagtaag agcaagagga gcttatgtag
    13021 tagcaatagc tcaaagccat aataaggatg ttgagaaagc tgctgataaa ataatctata
    13081 taccaaattc agatgatatt ttatcaccaa tattagcagt tgtaccaatg caattacttg
    13141 cgtatcatgt ctctgtacta agaggatgcg atgtagataa gccaagaaat ctagctaaat
    13201 cagtaacagt tgaataaaaa cagattaaaa attaaataga taatatttat tgaaaaaaac
    13261 tcatcctgtt atttgtattt tataggatga gttttttaga taaatattaa atttgttttg
    13321 tttttgctat aataaatttt ataaaaagat tgatattaaa tcatctgtaa aatgataaat
    13381 atatagacca taccaggtat agggaacgat aaatatagtt catataaaat atagtgagta
    13441 cttaataaac atatagtcaa tatcagacat aaaaaaataa tagcttatga gaaaaattaa
    13501 aatacaaggt attagtaacc ttgtgataag ataaagtgag tagtttaaaa taagaatata
    13561 aggaactatt tataaaagaa tataatgaag ctaatagagt ataagaaaat tactttattt
    13621 tagtaaaata atttcaaata aaaatttttt taaattggga gtataaatta tgacaaatga
    13681 ttttaaaaaa gaaaagaaag aagattattt tgttaatctt aaggcaatat ctactgaagt
    13741 tttacaagaa aaaatgcaag ataacgcttg ggttatagtt gatactcgct taaatgatgc
    13801 ttataatggc tggaagttag atggagtaaa aagaggtggg catataaaaa gagcagtaga
    13861 tttttctgct aattggttaa atgtatactc agatagaaaa gatgaagtgc tagaacaagc
    13921 attgaaaact aaaagaattg atttagataa aaatatagta ttgtatgatg caaatggaaa
    13981 agatgctttg gttgtagcag attatttatc taaaaaagga tataaatatc tatataaata
    14041 tgatattaaa caatgggcag acgatgaaaa tcttccaatg gaaagatata aaaattatca
    14101 aatgatagtt cctgctttta taataaaaga tatattagat ggaaaaattc ctgagacatt
    14161 tgaagattca aagaatataa aaatgattga agcgagttgg ggagaagaat cgtatacaaa
    14221 agggcatata cctacaagtg ttcatgtaaa tactgatatt atagaacctc ctccaacatg
    14281 gatgcttgat aatgatgata acttaactaa gtttgcttta gactatggac taacaaagga
    14341 tgatactgta atagtttcaa gtattacacc tatggcatct tatagactag ctgttatatt
    14401 gagatatata ggagtaaaag atgtaagagt tttgaatggt ggaacaaact catggttatc
    14461 agcaggatat gaattagaat ttattagtaa tccaaaacat tcatgtacta attttggagc
    14521 agatatacca gttaactcac aattagttgt tactacatct gaattaagac aaaaactaaa
    14581 agaaaaaaat aagtttatac tagtagataa tcgtacttgg gatgaacata taggaaaagt
    14641 atctggatat acatattatg ataaaaaagg gcgtatacca ggagctttat atggacattc
    14701 aggtagtaat agtgtttcac tagaagaata tcgtaatatt gataatacaa tgagaaataa
    14761 atctgagatt ttagaaatgt gggacaagga aaatatagat gtgaataaac agcttatatt
    14821 tatgtgtgga agtggatggc gtgctgctga agtattaaca tatgcaaatg tcataggagt
    14881 ggaaaatacc tccctttata gtgatggatg gatgggctgg agtcttgata attcaaattt
    14941 aattgaagtg ggagaacaca aataaagttt ttgaaagtac aatagagttg tttaaaacaa
    15001 aggtgttttt gatttattag gaagagtgtg taaattgtgt attgggaagt gtacttaggt
    15061 atgttcattt ggagctatgg aaattgataa aaaagtatta tctgtttatt ttgctggtaa
    15121 aatatgaata gattatagat tatagattat agattataga ttatagatta tagattatag
    15181 attatagatt taagtataga ctaagtaatt tatattacag aagataatat ataagaatta
    15241 atgcaagatg tatttgatac atataaggaa ttggcaagtc caggagaagg atagagaaaa
    15301 ttgagattga agagtttgaa agagattatt agatgaaaaa tacaaaaatg tataaaaata
    15361 catattgaga aagtatatct ctcttaaatt tgttaataat tctattatac agaaaaacat
    15421 atttaagggg gattttttac tatgaaaaaa gtaaaattag ctataagctt ttttatatta
    15481 atgattatag gaatgacaat atcttatgca gatacaagta taaatactag atacaataga
    15541 ttaagtaaag ctcttgaaat atcagaagca aattttaaat tttataatgt taaaattaat
    15601 tctgttataa atcataattt atcaaataaa gaatttcata atatttttat agaaataatt
    15661 aacaatctaa agttggataa aaaaggaata aattggaatc aaaaagggtc tggcgataaa
    15721 atacaattat gtgctactat aaaaggtagc gataaagaaa tttctataaa aggaataaat
    15781 aaaaataaca aagaaagtta catagtagtt gacatattgg ataacaaagt atataaacat
    15841 aaagaggtta tttatagtaa cgtagaaaat gtgttaaata aatattcatc tcaagtagat
    15901 gtgtctgctt gtattgttgg agaatacaca aaaaggttac aaaacaacaa atatgatgat
    15961 attttacaaa aaatattata taatatgagt gctaagcaaa tagatgaagt taaagacgta
    16021 aactttttat ctataacagc atatagtaaa gtattaaatg attatgaatt agaatattta
    16081 ggggaaaaaa ttaatttaaa tataggaatt aaatataatg aggatgacgg aaaaacactc
    16141 atatatattg ccacaccaat tataaaatta gattattaat tatgaggaga tcaaaaaata
    16201 tggctaagat aatagtaaaa aagagtaatc cacttaaagg tagtgttaaa atagatggtg
    16261 caaaaaatgc agttttacca ataatagcag cgactttatt agcaaacgga aaatcaacat
    16321 taaatggagt accaaattta agagatgttc atgttatatc agatttatta agacatgtag
    16381 gagcagaagt tgaatataaa gaaaatacac ttactgttga tgcaagcaat ataaaaactt
    16441 gtgaagctcc atatgagctt gtaagaaaga tgagagcttc ttttttggtt atgggaccat
    16501 tgcttgctag atttaatagt acgaaaattt ctatgccagg aggatgtgct ataggaacaa
    16561 gacctataga tttacattta aaaggattta aagccttagg tgcaaagata gaaatggacc
    16621 atggttttgt tgaagcagca acagaaaagt tagtaggaaa taaattatat ttagatttcc
    16681 catctgttgg agcaactgaa aacataatga tggcagcttc tttagcagaa gggactacaa
    16741 taatagagaa tgcagcagaa gagccagaaa tagtagattt agcaaacttc ttgaacgaaa
    16801 tgggagcaga tgttaaaggt gcaggtacta atactataaa aataaaaggt gttaaagagt
    16861 taaaaggagc agaacataat gtaattccag acagaataga agcagcaact tatatggttg
    16921 cagcagctat gacaaaaggt gatataactg ttgaaaatgt tttaatggag catttaaaac
    16981 cagttgtagc taaattaaga gaagctggtt gcgaaataac agaaatggac aattctgtaa
    17041 gagttgtagg tccaaaagta ttaaaaccaa tagatataaa aactttacca catccaggat
    17101 ttcctacaga tgttcaagca caatttatgg ctatgcttac tgtagccaat ggaactggtg
    17161 tagttataga aactgttttt gaaaatagat ttatgcatgt tgctgaattt aatagaatgg
    17221 gtgctaatat aaaaatagat ggaagaagtg cggttgttaa tggtgtagat gagctacacg
    17281 gtgcagcagt aaacgctact gatttaagag cgggggcagc ccttatttta tgcggtctta
    17341 tagcagaagg agaaactcaa ataggagaaa tataccatat ccaaagaggt tatgttgata
    17401 tagataagaa gataacagca ctaggtggtc aaatcgaaat agtagaagat taataattgg
    17461 tttatagaag aaagttataa attaataggg ttgttcatat acttaaatac aaggttttaa
    17521 aagctaagga ggagagtata tgaagaaccc attggttgtt ttgttaggat ttgtgacttg
    17581 ctctgtgtta gttccatcac ttataacttt agtttcttat aaaaatgtag aattaactga
    17641 aaaaccagag agcccagtat ctataaacaa aactattaaa aaaagtgata ttgaagacaa
    17701 aggtaataaa gaagaaaaaa gtacagtgag ctatgaaact gtaaataaaa aagcaccaat
    17761 tatcaatgta tataatcata taacagggaa aaccgaaaag atggacatgg agaactattt
    17821 atgtggagta ctagctggtg agatgtcttc agagtttgat atagaagctt taaaagctca
    17881 atctgtagca gctagaacat atgtagtata taaacaggaa catggtaaat ctagtaaaca
    17941 taaaaatgca gtagtatgta ctgattataa acattgtcaa gaatataaga gttatgatac
    18001 tcttaaaaaa ttaaatggtg aagaatggat taagaataaa tattccaaaa tccaagaagc
    18061 agttagagga actaaaggtc aaataataac ttataatgat aaggcgatac ttcctcttta
    18121 tttttctaca tcttcaggaa aaacagaaaa tagtgaagaa gtattttctg caaaatatcc
    18181 ttatttaaag tctgtggaaa gtccttatga taagtattcc cctaaatttg cgtcaacact
    18241 taaaataagt aacacagatt ttgttaaaag tcttagaagg gcatatagca ctattgtgat
    18301 agatgtgaat aatttaagta aacaagtttc aataactaaa agaagtgatg ccggaactgt
    18361 agaaaaaata aaattaggaa ataaagaatt gactggaaaa gacattagaa ctgtttttaa
    18421 attgaactct gcaaactttg acataaaatt tggagaagga tacatagatt tcgtggtaaa
    18481 aggatatggt catggtgttg gtatgagcca gtggggagca gaaggaatgg cagaagaggg
    18541 ctataaatat tatgatatat tatcacatta ctacacagat acaaaaataa aagatatata
    18601 ctaataattg tataaaatgc caaaaacacg tcaataatcc aaatgtagaa atatatttgg
    18661 aggtgcaaaa tgaagaaaaa gctgttagaa aaagatggtt tttatttatc tttgtttgta
    18721 tgtgtttgtt tattagcagt aggtggagtt tggtttacaa ataataatgt agataaattg
    18781 gcttctaata aaggaattat ggaaaatgct aataaagata gtgaagagga aatacattta
    18841 attgaaaaag acaagaaaga tgctatccct actgcaacgg attcaaaaca aaacttagaa
    18901 aaagctaagt caaaagagga aaataaatcg agtacaacaa aattaaatta cattggagat
    18961 aaagtaataa gaggatattc agaaaaagaa cctagttatt ctaaaactct tgatgtttgg
    19021 gaaactcata aaggtgtaga tattagttgt actaaaggca aagaagtaaa atctctatta
    19081 aacggaatag tagtagatgt atttaatgat gaagaatatg ggcaatcagt caaaataaaa
    19141 agtgacaata acattgtagt agtatattca aatctagata aaaatgttag tgttaagaaa
    19201 gaacagaagg taacagaagg acaatctcta ggtactgtag gtagtacttc tcaaatagaa
    19261 agtgaagaag gtatacatgt tcatttagag gcgtatagtg gagaaaaatc tatagaccca
    19321 atgagtctaa ttaagtaaaa gtataacaag tttaatcata tttggttaag cttgttatat
    19381 tttttggtgt ttttacatat atatcattaa agctattatg tgaggggggt aatctttgag
    19441 atctcatata gaggaaaggg ccatagtggt agcaaaatat atacttgaaa aaaacactac
    19501 agtaagacaa accgccaaga catttggagt aagtaaaagt acaattcata aggatgttac
    19561 agaaagattg aaggaaataa atccatccct tgccaaagaa gttaaaaatg tgctggacaa
    19621 aaataaatca gagagacata ttagaggggg attagcaacg aaattaaaat atgagaagga
    19681 acataaaaaa atgtaggtta aaaacctact ttttttattg aattaaatta taaaaagaaa
    19741 tatattaagt aattataaat agcaaaataa taatactctc aaaagattta ttttctcttg
    19801 attaaagcga gatttttgta tatgctttat tataggagta ttaaaatagg cttataatat
    19861 aatctaatat tataaataaa ggagttgaaa aaatggctgg agctgatata ggcatagatt
    19921 taggtactgc aaatgtgctt gtttatgtta gtggtaaggg aatcgtttta gaagaaccat
    19981 ctgttgtggc gatagacaaa agaacagatt ctgttttagc tgtgggagaa gaagctaaaa
    20041 agatgatagg gagaacacca ggtaatatag tcgctataag acctttaaga gatggtgtaa
    20101 tttcagatta tgatgtaact gagaagatgt taaaatcttt tatagataag atagttgata
    20161 aaaagggatt tggaagattt tttatgccaa gaattatggt atgtgtacca acaggtgtca
    20221 cagaagtgga aaaaagagca gtagaagatg ctacgagaca agctggagct agagaagtat
    20281 atatcataga agaaccaata gcagctgcca taggagctgg tgtagacata tcacagccta
    20341 atggtaatat gattatagat attggtggag gtactgtaga tatagctgta atatctctag
    20401 gaggagctgt tgtaagtgaa tctataaagg ttggtggaga tagatttgat gatgctatag
    20461 ttaaatatat gaaaaaacaa cacaatcttc ttataggaga aagaacagca gagaaaatca
    20521 agtttgaaat aggttctgca tttaagagag aagaagaaaa atatatgaag ataacaggaa
    20581 gaaatttaat tacaggactt cctagttcta taactataaa ttcaacagaa atgttggaag
    20641 cacttagaga atgtgttgag caaatagtag ttgctactca tgctgttttg gaaaaaacgc
    20701 ctcctgagtt agcagcagat ataggagatg tcggtataat aatgactgga ggaggttcat
    20761 tattatatgg attagataaa ataatagaac agaggacagg aataactgtt acaatagcag
    20821 atgaaccatt atcttgtgtt gcaaaaggta caggaatagc tttaggttca atagacttac
    20881 ttgaaactgg tgggtctttc aaaagaaaat aattaggaga gtttagaatg ttaaatatag
    20941 atgaaataaa aaaattaata ccacatagat atccattttt attagtagac aagattacag
    21001 agctagaagt tgggaaaaga gctgttggta taaagaatgt aacagttaat gaaccatttt
    21061 tccaaggtca ctttccagaa tatcctttga tgccaggagt actaatagta gaagctctag
    21121 cacaagtatg tggagtggca atgatgtctg tagaggaaaa caaaggaaaa ctaggtgtat
    21181 ttgcaggtat agataaagtt agaatcaaga gagaagtaag accaggagat acattaacta
    21241 tggaaattga aatgacaact cttagaaaaa atatagctaa ggcagatgct aaggcatatg
    21301 taggtgaaga acttgtctgt aaaggtgaat taatgtttgc tttagtagaa aaataaaaac
    21361 ttgaaatata aaagtaaaaa ggtagataat ttggagtatt taatcagata ctctaaaata
    21421 tctacctttt tatataaaga attttatcat ttagtttata tttagattag gtggatacta
    21481 ataaagccaa ggctattgtt tcagctaaat ctaatataaa gctaaggcgt atattattaa
    21541 atgaaagttt attattttca tcaattttat ctacaattcc tactatagaa taatttccta
    21601 tttgaggtag ttttttacca acaccttttc cgggcaggat aggtccttct ctaatctgta
    21661 tattacctat gttactctgc gaccccaagc aagcatctat agccaagaag ttaccttgta
    21721 tatgtgtatt ttttatagta tctaaagact catatatatt taaggcgtga atagggttat
    21781 ctagtgtacc gtaaacgggg tatttaaagt tactgttctt aagtattgtt ccaactaaag
    21841 ggcctaaagt atcacctata gccctgtctg ttcctatgca aacaacaatc gtattttcat
    21901 taattatagt ttttagaact gaacttagca tctgtataac atcatcatcg ctgtagttta
    21961 ctttagctaa atacaatagg aacaccccct tcaaaaaaat atatgaataa aaagcattaa
    22021 aaagacctaa ctttaaattt cagatagata gaaaatttta tttgtcttac aaaaaaagga
    22081 atagaaaaaa tatgactaaa aatattgaca aaattttaaa aaaatagtaa gctagtaaag
    22141 taaaacaaaa cttaaatctt gattacctta tcaagagagg ctgagggata ggccctgtga
    22201 agcccagcaa ccacccaaat gggaaaaggt gctaattcct acaagacaaa agtcttgaaa
    22261 gatgaggtca gttaattagt atgtaattac gattattaac ctcttcttat aaaggagagg
    22321 tttttttatt ttgagtaaat ttctaaaatt aaaaataagg taaaacctat aataaaaatt
    22381 aagaataaat aagggggaat aaaaatggca agacatttat ttacgtcaga atcagttaca
    22441 gaaggacatc ctgataagat atgtgaccaa atatcagatt caatattaga tgcattatta
    22501 gaaaaagacc cacaatctag agtagcttgt gaaactacag ttactactgg tttagtttta
    22561 gtagcaggag aaataagtac atctgcttat gtagacattc ctaaattagt aagagaaaca
    22621 gtaagagaaa ttggatatac aagagcaaaa tatggatttg attgtgatac ttgcgcagtt
    22681 ataacttcta ttgatgaaca atctggagat atagctatgg gagttgatga aggtctagaa
    22741 agtaagactg gagaagagat agaagaagaa atagaaaaag ttggagctgg agaccaaggt
    22801 ataatgtttg gatttgcatg taatgagact ccagaattaa tgccacttcc gatatctttg
    22861 gcacataaat tatcaagaag gcttacagaa gttagaaaaa caggtttagt tgattattta
    22921 agaccagatg gaaaaactca agttactgtt gaatatgaag gaagtaaagc tgtaagagta
    22981 catacagttc ttatatcagc tcaacattgt gaaacagtat caaatgataa aataagagaa
    23041 gatttaatta atcatgttat taaggaagtt ataccagcag aattacttga tgaagagact
    23101 aaaatttata taaatccaac aggaagattc gttataggag gaccacaagg agatacaggt
    23161 cttacaggaa gaaaaataat aatagatact tatggtggat attctagaca tggtggagga
    23221 gctttctcag gaaaagaccc tacaaaagtt gatagatctg ctgcttatgc agctagatat
    23281 gttgcaaaaa atattgttgc ggctggtctt gcagacaaat gtgagataga acttgcatat
    23341 gctataggta tagctagacc attatctata tttatagata cttttgggac aggcaaggtt
    23401 tcagaagaaa aacttgttga attagtaaat aaacactttg atttaagacc gggagcgata
    23461 ataagagact tagatttaag aaagcctctt tataaaaagg ttgctgctta tggtcacttt
    23521 ggtagaactg atatagattt accttgggaa agaactgaca aggttgagca gttaagaaaa
    23581 gatgctttag gtgaataatt tttaactaaa aagctgttag aatgtaattt tacgattact
    23641 ttctaacagt ttttttatta tgagttaaat atttttaata aatgttaatt tatcatcata
    23701 taatgaataa acagtattta atctggtcaa tacattttat ataatactct actaaattta
    23761 tacatattat gggatatatg ttaaaggatg gttaagaatg tgttaactaa tgttaaataa
    23821 ttaaaaatat attgaaatat cacaataatg agtctaatat aatcttaata aacttacact
    23881 ttaaaaataa ttttaattgg gggggagtag tgtatgtcag gcaaagtaaa tattaaaacg
    23941 gttataagtt ttgctggagc ttatgttgct actgtaatag gttctggatt tgcaactggt
    24001 caagaaatac tgcaattttt tacgttttat ggatatgcgg gtattatagg tggaataatc
    24061 tctatggtac tattttcatg gtttggagca gaagttatag ataagggaag agaattgaaa
    24121 cttaaggagc caataaaaat ttatcaagta tattgtggaa aatatcttgg aacatttttt
    24181 gaatggtttg gtccattgtt tttatttgga acattagtaa taatgattgc aggagcagga
    24241 gctacactat cagagtacta tggattaaat ccatatgtag gtaggattgg tatggcaatc
    24301 gtttccctaa taactgtttc attaggtttg acaaggcttt ctaaaatact aggtaatata
    24361 ggacctataa taataatatt tacactttta gtaggagcaa ttagtttatt tagcaatatt
    24421 gatgctttat caaatgctgg aaatatggtt gatagtttaa atattaaaac tgctacatca
    24481 aatggatatt tctcgggagt actttatact tgttacaatg taataatagt aataacattc
    24541 ttgacaggaa tgggagcttc tgcagtaaat aagaaagatg ctgtatgggg tggtatagta
    24601 ggtggagtag cacttatggc agcagctata atgatgaatt tggcattact ttcaaacata
    24661 ggaaatatat acacaaaaga gataccagca ctttacttag ctgataagat atctccaata
    24721 ataggtattt tattctcagt agtactttta ctaggaatat acactacagc tgtaccattg
    24781 ttatggtcag taactaaccg atttgtagaa gatgaccacc ctaaatttaa aattataaca
    24841 atagtggtaa gtatattagc ttgtatagga ggtttactgc catttgataa gttagtaggt
    24901 acactatatc catatacagg ttacatggga attttaattt tactatgtat gctttataga
    24961 agaattacta aaactgaggg gtacaaggag aataaatctg aaataagtta gtactgtaat
    25021 atacttatgg aatttaattt atactctaaa tatagagtaa ttaaaataaa atagctattt
    25081 aaatatggta cctgtatttc atggtaccat attttatgga tgcagatttt ttttgtttct
    25141 gataaaatgt ataatagttg gaaattacgt taagtataaa aatataaaac ttttctacga
    25201 ttatcatagt tttaatatat aaaattatac taacatttaa atggagcgat tattagtatt
    25261 tttaggagat aaaaatggaa aaattagaag gaatgataag tgaaatagtc tttaaaaacg
    25321 aagataatgg ttatacaata gctcacctgg taaatgaaaa tgatgaaatt gtggttgtag
    25381 gatgtatgcc tactttggca attggagaaa gcatagaagt tgaaggaaag tgggtaaatc
    25441 ataagattta tgggactcaa tttgaagtga atagttttat gccagtaacg ccatcatcct
    25501 tagaaggaat ttatgtttac ttatcttctg gaatgataca tggtattggt gaaaaaatgg
    25561 caaaaagaat aattgataag tttggtgtag atacgttaga ggtgatacaa aattcaccag
    25621 aaaaattaca agaagtagaa ggtataggga gtaaaaaagt aaaacaaata gtaaaaagtt
    25681 atgaagaaga cagagaattg agaaatataa taatacagct atctcctttt ggaataacac
    25741 caaattactg cctaaaaata tataaaaaat ataaaagcag tgcaatagaa gttataaata
    25801 aaaacccata ccaacttgct gaagatataa gaggaattgg atttaaagta gctgatagta
    25861 tagcaagtaa aattggtata gataaaaact ctaaagatag aatttgtcag ggaatcttat
    25921 acactttaaa taaatcttta agtaatgggc atacatattt accagagcat gttctaatac
    25981 aagattcgga aaaattattg gagctaaatg gagaaataat aaaagagtgt gtaatgatgc
    26041 tagtttataa tcaaaaaata catatagaga aagtaaacaa tgaaaatctt atatacctta
    26101 tgccatacta tttagctgaa aatggagtat gtagtcaaat agtaaaatta tcacagtatg
    26161 aatttgaaga cttaaaaata gatatagata acgagattaa tgttttagaa gaagataaga
    26221 aaattaagtt agcagaaaaa caaattttag cagtcaaaga atctgttaat agtggagtat
    26281 taattataac tggtggacca ggaactggta aaacaactac cattaatgct atcatagata
    26341 tatttgaaaa taatggtaaa agtgtgactt tggcagctcc aacgggaaga gcagcaaaga
    26401 gaatgagtga aacttctaat aaagaagcaa aaactataca taggttgcta gaaatgggat
    26461 tttcaacaga tgatgattta acttttttta aggatgaaga agacccaata aattcagatg
    26521 taataattgt tgatgaggta tcaatggtag acattatctt aatgtacaat ttacttagag
    26581 ctataaagtt aggaacaaga gtaattttag ttggagatag tgaccaatta ccttctgtag
    26641 gtgctggaaa tgtacttaag gatatgatta attctaatat tataaatgta gttaaattaa
    26701 atgaaatatt tagacaagcg caagaaagta tgataattgt aaatgcccat aagataaaca
    26761 atggagaacc actttatcta aatacaaaag gtaaagattt cttttttata agaaaatcaa
    26821 ctaatgaaga aattttaaat gaaataatag gattggttaa tgaaagatta cctaaatttt
    26881 ataaagttga taaattaaaa gatatacaag ttttatcttc aatgagaaaa ggtgaattgg
    26941 gagttactaa tctcaatata gagttacaga agtatcttaa caaaaaagaa aaatttaaag
    27001 ttgaagaaag tttttctaaa agattattta gagttggcga taaggtaatg caagtaaaaa
    27061 ataattatac aaaaaaatgg gaaacagaag accaaaaaga aagtggagaa ggtatctata
    27121 atggagatat aggatatgtt tatcacatag ataaagataa gaaaactatt tatatattat
    27181 ttgaccagac taaaattgta tcatacctat atgatgaatt agatgaaatt gaccatagtt
    27241 tttgtacaac aatacataaa agccagggaa gtgaatttcc agttgtagta ttgccaatag
    27301 cgtgggctcc tcctatgtta cttagccgta atttattata tacagcagta acaagagcaa
    27361 aaaaattagt tgtattagtt ggagatgtga aatatcttga atatatgata aaaaataata
    27421 gagttaatca aaggtattca aatttaggat ataagctaaa taaatttaaa caagaagggt
    27481 tgttgataga ataaatatga aaatatgcaa tgataatttt attaagaatt ttaaaaatat
    27541 gattaataaa tgtttggatt ttatatatcc tgagaatata agttgcataa tttgtgataa
    27601 atccataaag aaaacaaata cttattcaat atgtaaaagt tgttttaaag aaatgaattt
    27661 tatacaagat ggttgcatga agtgtggaaa accaataata cgtcattcaa ttgaaaaaga
    27721 atttattgaa gagtgtagtt attgttttaa taaagacttt tattttgata aatcaatatc
    27781 ttgtattgaa tataatgatg tgacaaaaaa gatggtgtta gggctgaagt ataaccaaaa
    27841 aacctttatg gctaagtata tagctcaaat aatgaaagaa aaattatatt tagaaaacat
    27901 aaaatttgat tatatattat ttgttccact tcataaaaaa aggctaaata aaagaggttt
    27961 taatcaggca caaaaaatag catttaattt aagtaaaata gtaaatattc cattattaga
    28021 ttgcataagt agaaaaaaat atacaagaat gctatataaa ttgaataaga aagaacgaaa
    28081 agaagaatta aaaaatgtat ttgttgttaa agaaaatgtt aaattaataa ataataaaaa
    28141 tatactttta atagatgaca tatttactac tggtctaaca acaaacgaaa tctctaaact
    28201 tttaaagtta tcaggtgcta ataaggtttt tgtattgact ctacttacaa aagcaaatga
    28261 taattatatt atggaataat cttatatatt gacaatttta gtttaaaata ttaaaatgaa
    28321 taagtgatat ttaggtctgt ggttgaaagt ccaagccagt cgcaggcaaa gggatccacg
    28381 taagttaaca ctaaaaattg ggttaatgat catggtgcgg cttagaagta agacctaccg
    28441 ataataaaat cgagagagag tagtagtgcg gcgaacccaa gcgaaccctc cagtaggcga
    28501 gtgtggggtc aaaaaccagg tcagctaaat atcatattaa aagagagcta gcataccttt
    28561 atgtatagct cttttttata tataataaat ttaggttgag tcataaagag gtgatatgtt
    28621 tgaataaaag acaagaaaat ttattaacaa cattgctatt aaaagaagag tttataaatg
    28681 ttaataagat tgccaatgat tttgagtgtt cagaaaggac aataaggaat gattgcaaat
    28741 atattgatga atggttatct actttttgta atgcttatat caaaagaaaa cctaatattg
    28801 gaattaaatt tagtggagat ggaacagaca aaatactggt aaatcagaag ttgcgaggtg
    28861 aagttaaaag agtaaatagt gagcttcata agcaagttga gatattaaat ttactacttt
    28921 gtaataataa aaaagttact ttaaatgatt tatctaataa gttgtacata aataaaaata
    28981 ttgtcagaga agaaataagt aaattatcag tagcattaaa taattataat ttaaatgtct
    29041 cgactaaaaa aggcagtggt atatatattg aaggggatga gaaagacata agaataatgt
    29101 tagtatcatt cttatttaaa tacatagata aatacagatt aaacatagat gaaatagagt
    29161 acttccatat agtagatata ataattgtaa aaaacataat gcttactata gaggaggata
    29221 tggatataag acttacagat atatcattca agcaattgat gctatttttg cttataaata
    29281 taatacgaat aaggctggga aattctttaa aagtatcaaa tactaagaaa gagcttaagc
    29341 taaaaatgct tatattggat atagaagatg aattgaaatc taatttatcc atagtattaa
    29401 attatgaaga aaaattgttt atagaatctt taatattggg tatgaacaaa caaataaata
    29461 gtaataatat taagaaatct ttatatctaa atgaagaatt agcaagttat acaaaaaaaa
    29521 ttataaattt agtatcagaa gaatcaggaa taaattttaa taatgatata ttactttatg
    29581 aacaattaat atgtcatttg aatgttacaa tgcatcaaat gaaaagcaat gtttacttag
    29641 aaaatccact tttagataac ataaaaacta aattttgttt tttatttaca gtaatatcaa
    29701 gttcaataga aattgaatta aaaggaaaac aaataacgga agatgaaatt ggttatctta
    29761 cacttcactt tcaaacctct ctagaaagaa aatacagcaa aagggaatca aataaaaagg
    29821 caagtattgt atgtccattt agttttggag taagtatgct attaaaagta aagatagaaa
    29881 agagatttga taacataaaa attattgaaa cattaagaga ggaagattta aaaagagata
    29941 actttaataa gactatagat tttataattg catttcaaga gtatgaaaat atccaaaaac
    30001 caatttttat aacaacacca ttatttactg atgaggatga gaataaactt aaagagttta
    30061 caaacaaaat aaaagaaaaa gacacctcat ataaacttat gaacagatta atgatgtctg
    30121 attatttaat aagagagtta gaacatgatg atatttatga agctataaca tatttaacta
    30181 atttattaat tgaaaaacat tatgcagaaa aagagtattt acaatctatt ataacaagag
    30241 aaaagtcata tccaaccaat gttggaaaag gaatattatt ccctcatgga gacatgaaat
    30301 atataaaaca atctgtttta tgttttgcaa ggttaaaaac accaataaaa attagaaatg
    30361 gaaaggatat aaagattata cttttattag catataaaaa agatgatgat agaaaagtat
    30421 ttagagaatt atttaaagaa atatctaatt taacagagga tgaaaatatg ctagatattc
    30481 taagtaaatg tgacattgaa aaggtaaaag atattcttat ataaaattca taatgaacta
    30541 tggataagca caaaattttc catgtgatgg aaaaagttgt gcatgttttt ttgtttttat
    30601 aagatattat atgtttgtaa ggtataaata atctaaaagt agagaagcaa tttaggagga
    30661 aaaaatggaa gaaatagttt taaagattat aattcatgct ggaaatgcaa aatctatgct
    30721 ttatgaagca cttgactatg caaaggaaaa cgattttaaa aaagcagatg aattaattga
    30781 aaatgcaaat gaagaaattt taaaagcaca taaagtacaa actgaactta tacaaaagga
    30841 agctggggga gataaatcag atatatctat tttattgatt cactctcaag accatttaat
    30901 gacttgtatg agtgagagaa accttataaa agaaatgatt atgttaagaa aagaaattca
    30961 aaaattaaaa taaaaaaatt aagggggatt taaaagaatg aaaagaaaag tatatttatt
    31021 ttgtagcttt ggtatgtcaa caagtttact tgcagataaa atgcagaagg tagcagatga
    31081 acacaattta ccaatagaag tagaggcatt tccgatagca gaaattgaca aaatagttga
    31141 agaaaaaaat ccagattgta tattattagg accacaagtt aaatacatgc taaaggagtt
    31201 aaaaccaaag tttgaagctc aaggaaaact tatagatata atcaatgaag ttgattatgg
    31261 aactatgaat ggggaaaaag tattaaaact tgctataaag ctaataaaat ctaaacaagc
    31321 gtagaattat agtaaaaaat aataggaaat gagggtggat agatgaatag attggaaaga
    31381 atacttatgc cattggctga aaaaataggt aaaaataaat tattaattgc aattagagat
    31441 ggttttttaa tatcatcacc attattaata ataggttcat tatttttatt agtagcaaat
    31501 tttcctataa aaggatggaa tgaatttgta ggtcaaattt ttggtccaga ttgggctgta
    31561 aaattgcaac aaccaactgt agcaacattt gaagtaatga ctttacttgc agttttagga
    31621 attggatatt cttatgcaaa gcaaatagat gtagatccaa tagcatcagc tgcagttgca
    31681 atagttgcct tttttatagt aacaccgttt gtaataccat atacaccaga aggaacagaa
    31741 tcagtctaca tggtaacagg tataccacta ggttggatgg gctctaaggg aatgtttgta
    31801 ggtatgataa cagcaataac ctctgtaaaa ttatttgctg cagttgtaaa aaaaggatgg
    31861 acattgaaaa tgccagatgg agttcctcca accgtatcaa aatcatttgc agcattaata
    31921 ccaagtgcaa tagttatggt agttttcttt ttagttaaga tagcttttga agctacacca
    31981 tatggaagta tacatgaatt tatatttaag tttttacaaa tgccattatt aaaattagga
    32041 aatagtttag gtgctatggc aatcgcttat atattcttac atttattctg gttctttgga
    32101 ataaatggaa gtagtgttgt tggggctgtg tataatccaa tattaaaaat tctatcagct
    32161 gaaaacttag ctgcttttca ggctggttca aaaataccaa acataataac aggtcaattc
    32221 caagatatgt ttgcgacatt tggtggagca ggtagtacac tatcattagt catagctatg
    32281 ttattaatat gtaaaagtaa acgtataaag tcaatgggaa aactatcaat tttaccagga
    32341 atatttggta taaatgagcc attgatattt ggtctaccaa taatgcttaa cccaatacta
    32401 ttgataccat ttgcaatagt tccaacaata aatataataa tagcttactt ctgtatgtca
    32461 gcaggattag tgcctcttac aaatggagtt cagttaccgt ggacaactcc aattatattc
    32521 tctggttttt taacaacagg atggcaaggt gcagtcttac aattagtgct tcttatacta
    32581 ggaatattta tgtatatacc atttataaaa atgctagata agcaatactt gagagaagaa
    32641 aatgaaactg ttgaagagga agatgatgat atatcttttg atgatttagt attataggag
    32701 gaagattatg aagaaaatag ttatgatatt ggatcaaata caagctggag ctggaggaaa
    32761 agagaaaagt aatatacctc ctgctggaaa atcaagccca ttaggaccag gtgtaatgat
    32821 ggagcaattt ttaaatgaat ctaaggtaat agctacctta ttttgtgggg atgaattttt
    32881 tgtaaataat gaaaaagaag taacgagtaa gatgatagct atggtaaaaa aattaaaccc
    32941 tgatgtagta atctgtggac caagctttaa ttacgaaaac ttttctaaga tgtcagcaat
    33001 actgtctaaa aatataaatg ataaaacaga tataccagct tttgcagcta tgtcagaaga
    33061 aaatatagat gtgataaacg aatataaaaa tgacatatgt atagttaaga ctcccaaaaa
    33121 aggtggaatt ggtcttaatg attcattaaa taacatatgt aaattggcaa aagcaatagc
    33181 gaataaagaa gatataactt tgatgaaaga agaattttgt tattaataag agtattaatt
    33241 attagcaagt ttaaatatta tactatttat aataagcata cattagtaaa atctatataa
    33301 tttaaggagg ggaattttaa catgtgggga atgatagcga catggagaat ggccctagaa
    33361 ggaataactg aggcatcaga acatttagaa aaaggcatgg aagcaggtga tgctgtagag
    33421 cttgctgtaa gaagagttga agactatcca ttctataaat cggttggata tggtggactt
    33481 ccaaatgaaa attgtgaggt agaacttgac gctgcattta tggatggtaa cacattagat
    33541 attggcgcag ttgctggtat aagagattat tctaatcctg tcagcatagc taggaagtta
    33601 agttatgaga aggtaaataa ttttttagtt ggaataggtg ctgaagatta tgctcataaa
    33661 aatggatttg aaagaaaaaa tatgttgact gatagagcga agttacatta taaaaagaga
    33721 aaaaaagaga ctttagacaa aggattaagc ccatatgctg gtcatgatac tgttggtatg
    33781 atatctttag acaaaactgg aaaaatgtgt gcagctacat caacaagtgg attgtttatg
    33841 aagaaaagag gtagaatagg agattctgca ctatctggtt caggattcta tgttgatagt
    33901 agtataggag gagctacagc tactggtttg ggagaagatt taatgaaggg atgtatatct
    33961 tatgagatag ttaggcttat gaaagaagga aagcatccac aagaagcatg tgatattgca
    34021 gtaaatgaac ttgataaaca gcttatcaaa agaagaggaa aagctggtga tttatcatta
    34081 gttgccatga ataataaagg tgaatgggga gtagctacaa atatagttga tttcactttt
    34141 tcagtagtaa ctaaagaaga atctcctgct gtatatattt caaatagagt agatggaaag
    34201 actatacata aggttatatc aaatgagtgg atggaagcat atgaggctag aataaaagca
    34261 ccgatagaag taagtgagta aagtagtgtt acataattaa aatagagttt tataataagg
    34321 agatttttat gaatatcata agtaataaaa tagatgagtt aaaagaagat ttattaagtg
    34381 atattataga tattgtaaaa atacctagtg tcaaagggga gtcagaaaat ggcttccctt
    34441 ttggcgaaaa agtaggggag gcacttaata aagcattaga aatatcaaaa aaattgggat
    34501 ttaaggttag aaacttggat aattatattg gttatgcaga gcatggagat agtgatgatt
    34561 atgtttgtgt aattggacat gtagatgttg tacatgaagg agatggttgg aagcatcagc
    34621 cttataaggg agaagaaact aatggaagaa tttatggacg aggagtacta gacaataaag
    34681 gacctataat gtctgcctta tatggattat atgctattaa agaattaaac ttaaaattag
    34741 ataagagagt tagaataata tttggtacaa atgaagaaag tggatttgaa gatataccat
    34801 attatttaga aaaagaaaaa acacctatta tgggatttac accagattgt aaatatcctg
    34861 ttgtatatgg tgaaaaaggc atggcaaaga ttagaataaa aagtaaaatc aattatgaag
    34921 aagatgtata cctaggtttt atagaaaata tgtctgaaaa tgtgcttgtt acatataaag
    34981 agctgaatat agaaaatagt aatactatat tagatataaa ggtaaaatac gattttagtt
    35041 ataaattgaa agatgtatta gatgaaataa aagctagttt tccaaatagt atcgatatag
    35101 aggtaatttc taattttaat cctgtatatt ttgataaaga gagtaatctt gttaaaaagc
    35161 ttcagctagc ttatgaaagg gttacatcat tagatggaac tcccgtgaca actaatggag
    35221 gtacgtatgc aaaagtaatg ccaaatatag ttccgtttgg accttcattt ccaggacaaa
    35281 aaggaatagc tcataatcct gatgaataca tggatataga agatataata ttaaatgcaa
    35341 agatatttgc aaatgcaata tatgaattag caaaggagta gttatgttag aaataatagg
    35401 aatgtcagta gaagacgcta aaattataga agattgtggt gcagatagaa tagagttagt
    35461 tagtgcttta actgaaggtg gattgacacc aagttttggt cttatagaaa gtgttgttaa
    35521 cagtgtaaag atacctgtga acgtaatgat aagacatcat gcgaaaagtt ttgtatacag
    35581 taaagaagat ataagtataa tgcaaaaaga tataggtgtg gttaaagaga ttggtgctaa
    35641 tggagttgta tttggtgtat tggataaaaa caataatata gatgagaaaa atctaaatgt
    35701 tttattgaaa tgttgtgata atcttgatgt tacatttcat agagccatag atgaatcaaa
    35761 tacaatagat agtgttaaaa ttttgaagga ttatgataaa attacgaata tattaacatc
    35821 aggagggaaa ggaagtatag ttgataacat acaaatgata aagaatatga tgcttagttc
    35881 taatcacata aaaattcttt taggaggagg tcttaatttt aataatatag aaaagataaa
    35941 ggaattaact aaagcaagta attttcattt tggtacagcg attagaataa ataacagtcc
    36001 ttttgaagat atagatagac aaaagcttaa acaactcgta aatataattt caagatagta
    36061 tatgaatata aaatacttag tttaaataaa gagagtaagt taattataat ttgggtatat
    36121 gaatgttaat aggtattaaa tatgaaatta ttgttgagta tgctttataa cttttaagta
    36181 gttttaaata taaataatga aagaaaagat agattatgga ggttttagag ggtttatagt
    36241 tattaaaaaa ttataaaggt atattggcat ataatacaca taatttccta aaattaccat
    36301 cttatgacat gaattcacat attaataaac aattaatgga gatttttgtg gatagaaata
    36361 ggagtaaaaa tagctttaaa taaacaaaaa caagagttat taacagtttt atccacatta
    36421 tccacaggtg tttgtaataa tattaatcca cataagtatc aatgtaacta aaaatgataa
    36481 ataattctat caattgaata gaaaaaatca taaaagtaaa taataaaaag aggattatga
    36541 gagttcgtgt agaatatatt attaaagtat aaattttctt atactgacat aattgaaggg
    36601 agatgtattt atgaacataa ttatatctgg aaaacaaatc gaattaactg atgcaataaa
    36661 aaataaaatc gaaactaaac taagtaagtt agataattac attcatccag acactgatgt
    36721 aaaagttaca gtaagtgcga gaaaagcaag acaaaaaata gaagtaacaa tagctactgt
    36781 aaatggtcct ataattagag cagaagatat gcaggaaaat ttatacacag ctatagatgt
    36841 tgtatatgat aaattaagta aacaattaaa aaaatataaa aagagattac aagataaaca
    36901 taaagacaac aagagtataa gattccaaga ttcagagata aatctagctg atgaacaaga
    36961 ggattatggg gatgaaaatc aagaattagt aattcaaaga cgtaagaaat ttagtgttaa
    37021 gccaatgagt gaagaagaag ctgtattaca gatggaatta ataggacatg atttttatat
    37081 gttcaagaac atagattcag atgaaatagc agtagtttat aagcgtaata atggtggata
    37141 tggtataata gaacatgaat aaagcaattt agatttaatc atattagcta tcgataaata
    37201 aattatcgat agctttttaa attaatatta aaagtatagt ttttatggta aaatgaataa
    37261 tagtacaata aagaacgagg agagatgtaa aatatgagct ttatggataa tttattcaac
    37321 atggcagata aaaaagaatt aaaaaagttc aataaaactg ttgatataat agattcatta
    37381 gaacctaaat ttgaatctat ggcagactcg gaacttaaaa atatgacaaa tatatttaaa
    37441 gaaagattag caaatggtga aagtatagat gatatacttc cagaagcttt tgcagtagtt
    37501 agagaagttt ctaagagagt gttaggactt agacactaca gagttcaaat gattggaggt
    37561 atagtactac atcaaggaag aatagcagaa atgaaaacag gtgaaggtaa aactttggtt
    37621 gcaactgctc ctgtttattt gaatgcgctt actggaaaag gtgtacatgt tgtaacagta
    37681 aatgattacc ttgcaaaacg tgatagagac caaatggcaa aaatctatga gtttttagga
    37741 atgagtgtag gcgttattat acacggtcaa aatccaaaag ttagaaaaga acaatatgat
    37801 tgtgatataa cttatggtac aaataatgaa tatggatttg actatctaaa ggacaatatg
    37861 gtaatacaca aagagcaaag agttcaaaga ggattaaatt atgctatagt ggatgaggtt
    37921 gactctatac taatagatga ggctagaaca ccacttatca tatctggacc tggagacaaa
    37981 tcaactcact tatattcaga tgcaaacaca tttgttttaa ccttaaaacc agatgattat
    38041 gaattagaag aaaaagataa ggctgtatct ctaacagcat cgggtataca aaaagcagaa
    38101 gtgtatttta atgttgataa tataacagat atatctcata cagaattata ccatcatata
    38161 aatcaagctt taagagcaca tgtaataatg aagaaagatg ttgattatgt tgctaaagat
    38221 ggagaaatag taatagtaga tgagtttaca ggtagactta tgtttggtag aagatattca
    38281 gaaggtttac atcaagcaat agaggctaaa gaaggtctta aaatacaaag agaatctaaa
    38341 acactagcta ctgtaacatt ccaaaactac tttagaatgt ataaaaaact ttctggtatg
    38401 actggtacag caaaaacaga agaagaagag tttaaagcaa tttataaaat ggacgttttc
    38461 caagtaccta ccaataagct tatggtaaga gaagatttac cagattctgt atacaaaagt
    38521 gaaataggta aatttaatgc tgtagcacaa gaaataattg aaagacataa agttaatcag
    38581 ccaatacttg taggtacagt atcgatagaa aaatcagaat tattatcaca aatattaaag
    38641 aaaaaaggca ttaaacatga agtattaaat gctaagcatc atgataaaga ggcagagata
    38701 atagcacagg ctggtagatt aggcgctgtt acaatagcta caaatatggc tggccgtggt
    38761 acagatatag ttttaggtgg aaacccagat ttcttaacta agagagaaat gagaagaaat
    38821 ggatttaaag aggaaatagt aaatagggta gatactccaa tagaaggtat acctgtaaaa
    38881 ggtaatgaaa ttctatttga agctagagaa gagtatgaaa aactatttga aaagtttaag
    38941 caacaaacac aagaagaaca aaaacaagtt gtagaagcag gtggacttgc tataataggt
    39001 acagaaagac acgaatctcg aagaatagat aaccagttga gaggtcgtgc tggtcgtcaa
    39061 ggtgacccag ggagttcaag attctatata ggtcttgatg atgaccttat gagattgttt
    39121 ggtagtgata gaatatctgg tattgtagat aaaataggtc ttgaagaaga catgccgata
    39181 gaacatagaa tattatctaa gtctattgaa ggagcacaaa agaaagtcga aggtaaaaac
    39241 tttggaataa gaaagcatgt acttcaatat gatgatgtta tgaataaaca aagagaaata
    39301 atatatgcag agagaaaacg tgtattagaa ggtgaagatt tacaagaaca aatacaatca
    39361 atgactcatt ctataataga agaagcagtt actctttata cacaagataa aggatttgat
    39421 gaagaaggat tcaaagaaca tatgtataat ctgttcttgc caaaaggtag catagaaata
    39481 ccagaaatag aaaaacttaa tccagttgaa ataacagaaa aagtttatga aatagctatg
    39541 aagatatata cttcaaaaga agaacaagtt ggctatgaga gaatgagaga agttgagaga
    39601 gtaatcttac ttcaagctgt tgataaccat tggatagacc atatagatgc tatggaccaa
    39661 ttacgccaag gtattggtct tcgtgctgta ggtcagcaag acccagttat tgcttataaa
    39721 atggaaggtt ttgatatgtt tgatgaaatg aataaacata tcaaagaaga cactgtaaga
    39781 tatttattta atataacaat agaaactcca gtggaaagaa aagcagttgt agatgttgaa
    39841 aatctatctt ctccatcaga tggaacactt ccaacaagta aaactgttaa aaaagatgaa
    39901 aaggttggaa gaaatgactt atgcccttgt ggaagtggta aaaaatataa aaactgttgt
    39961 ggaagataat ctcggaggat taaatgttaa agttacaaga gtatagacat agtatggagc
    40021 agatggcttc aaatttagaa gaattgaggg tttctctttg acatagcttc attgatgaaa
    40081 aatatacaaa ttaatgaaga aaagatgaat caacaagact tttggaatga taatgaagtc
    40141 gctcaaagag tacttcaaga aaataaatct ttaaaagaga cattagaaga atatgaaagc
    40201 ttaaaaagct tattagaaga tatagaagta ttaattgaaa taggtttaga agaagatgat
    40261 gattctgtag aaagagatat agaaaagtcc atagaatcaa tggaagaaaa attatctgaa
    40321 atgaaaataa agactcttct aaatggagag tatgataaaa ataatgctat attatctata
    40381 aatgctggta ctggtggctt agatgctcaa gattgggctc aaatgctttt gagaatgtat
    40441 ataagatggt cagaaagtaa gggttataaa gttaagcttt tagatataat atcagaccct
    40501 gaagcaggga taaagacagc tacaattctt gtagaaggaa caaatgctta tgggtattta
    40561 aagagtgaaa aaggagttca tagattagtg agaatatctc catttgaccc atcagggaaa
    40621 agacatactt catttgcatc tatagatgta acaccagagt tagatgaaaa tatagaagta
    40681 gaaattaatc cgtcagattt aaagatagac acatatagag cttctggagc tggagggcaa
    40741 catgtaaata ctactgattc agcagttaga ataacacata tccctactgg tgtagtagtt
    40801 cagtgccaaa atgaaaggtc tcagcatcta aataaggaca gagctatgag acttttaatg
    40861 gcgaaattga tagaattaaa agagttagag caaaaggaaa aaatagaaga tatacaaggc
    40921 aaatactcac aaataacttg gggtagtcaa attaggtcat atgtttttca accatataaa
    40981 ttggttaagg accatagaac aaatgctgag tttggaaatg tagatagtgt aatgaatgga
    41041 aatatagact tgtttattaa tgaatactta aaaatgaata aaatagttta ataaatatgc
    41101 actgtaatta agatatatgt ataagaaggt tagtgcttaa tatgcactta cagcacttag
    41161 gctgaggacg ataaccctca gccttcgttc tgttttgtta tacttatgaa aaactaacat
    41221 taggagtgaa taaattggat ataaatcaaa tcttaaaaaa agagtttaat cttagagatg
    41281 aacaaataaa taatacatta aaacttattg atgaaggaaa tacaatacca tttatagcta
    41341 gatatagaaa agaaatgact ggcgaaatga gtgatgttac actaagagaa ttttatgaaa
    41401 aattaatgta tttgagaaat cttcaaagta gaaaagatga tgtagttaga ctaatagatg
    41461 aacaagggaa attaacagat gaaataacac aaaatataga aaaagcaaaa acattacaag
    41521 aagtagaaga tatatatgct ccatataaac aaaagaaaag aactagagca acaatagcaa
    41581 aagaaaaagg attagaaaat ttagcattgt caatactaga gaataattta gataatatag
    41641 aaatagaggc aaagaattat cttgatgaag agaaagaagt tctttctatt gaagatgctc
    41701 taaaaggtgc tagagatata attgcagaac tagtctcaga tgatgctaag attagaaagt
    41761 atataagaga attagcttta agagaaggta tgatagttag caaaagtgct actgatgaga
    41821 aatcggttta tgatatgtat tatgattata acgaagcagt aaaaagtatg gctccacata
    41881 gagttttggc aataaacaga ggagaaaaag aaagcttcct aaaggttaag ttagaaataa
    41941 ataatgataa agttttaaat tacataataa acgaatatgt aaatgataaa aatttcaaaa
    42001 ataaagaaga aatcgtaagt tcaatagaag attcttataa aagattaatt ttcccatcaa
    42061 tagaaagaga aataagaaat catttaacag aaatagctca agaaagagct ataagtgtat
    42121 ttggaaagaa tgttaaaagt ttattgcttc aaccaccagt taaagacaaa gtagttatgg
    42181 gatttgaccc tgcatttaga actggatgta aaatagctgt agtagataaa aatggtaaac
    42241 tactagatta tacaacagta tatccaacag accctcaaaa tgatgtagag ggagcaaaga
    42301 aagttttaaa aggattgatt gaaaaatacg atatagatat aatttcaatt ggaaatggta
    42361 ctgcatctag agaatcagaa acttttgtat cagaaatgat aaaagaaata gatagcgaag
    42421 tgcagtatgt gatagttagt gaagcaggag catctgttta ttcagcatct gaacttgcaa
    42481 atgaggaaca tccagatata aatgtatcaa taagaggagc tatatctata gctagaagat
    42541 tacaagaccc attagcagag cttgtaaaaa tagaccctaa aagtattggt gttggtcaat
    42601 atcagcatga tttaaacaaa aagagattag aagaagtgct agatggagtt gttgaagatt
    42661 ctgtaaatag tgttggagta gatttaaata ccgcatcata ctccctacta gaacatgttg
    42721 caggaataag taaagctata gcaaaaaata taatagcata tagagaagaa aatggagatt
    42781 ttacatcaag agcacaattg aaaaaagtaa aaagactagg acctcaagca tttactcaat
    42841 gtgctggatt tatgagaatc ttagagggta aaaatccact agataataca ggtgtgcatc
    42901 ctgaaagtta tgatatatgt aaaaaaatga ttgagataat tggatattct ttagatgatg
    42961 taaagaataa gaatataggt gaaatagatg aaaaaataaa agaaattggc ttgagggaat
    43021 taagtgaaaa actggaagta ggacaagtta cgttaaaaga tatcatagct gaaatcaaaa
    43081 aaccaggaag agacccaaga gaagaaggta taaaacctat acttagaact gatgtcttaa
    43141 aaattgaaga tatacaagag ggtatgactt taaaaggtac aataagaaat gttgtagact
    43201 ttggtgcatt tgtagatatt ggaataaaaa atgacggatt agttcataaa tcagaaatga
    43261 gtaatagttt tgtgaaagac cctatgagta ttgttacagt aggtgatata gtagatgtaa
    43321 aagtcattgg aattgactta aacaaaaaaa gagttgcctt atcaatgaaa aaatagataa
    43381 gtttatgtat ggttatagta cttaaattct atatttgtaa atgagattac tcgagtgtat
    43441 aaatgttaaa aatttgtaaa agtatatagt aagaaaatat attaaaataa gaacgatagt
    43501 cttgaaaatc aggaggaaaa tgaatgattt ttataaaaaa tggtaaaata aataccataa
    43561 caaatgggat aattcatgga gatatactta tagatgaagg aaaaataata gaaattggag
    43621 aagatttgat agctccttta gatgtagagg taatagatgc tagtaataaa cttgtgtttc
    43681 ctggatttat agatgctcat actcacttag gtttatggga agatggtata gggtttgaag
    43741 gagcagatgg aaatgaagag actgacccaa taacaccaca attaaatcct atagatggaa
    43801 ttaatccaat ggatagaact tttaaagaag cttttgaagg tggaataact tctgtttgta
    43861 caacccctgg aagtgcaaat gttatgggtg gtcaatgtat agctataaag acatgtggaa
    43921 aaagaattga taaaatggta ataaaaaatc cagttgcatc taaaattgct tttggagaaa
    43981 atccaaagag ctgttatggt caggatgata aatctcctca aactagaatg gctatagctg
    44041 ctttacttag agaaaaccta aaaaaagcag aagaatattt agaggatatt gatatgtatg
    44101 agagtcatga tgatgaagac tgtgaaaaac ctgaatatga tataaaaatg gaaagtttaa
    44161 taccagtttt aagaagagaa attcctttta aggcacatgc tcatagagca gatgatatgt
    44221 ttacagcaat aagaattgca aaagaattta atttaaagct tacattagac cattgtacag
    44281 aaggtcatct tattgtagat gaattagtgg aagaagggtt tcctgtaata gtagggccat
    44341 ctttgtcaga aaggtctaaa tttgagctta gaaatttgac atttaataca gcaggaatac
    44401 tttcaaatgc aggtcttgat gtatgtataa tgacagacca tccagtaata ccagttcaat
    44461 atcttccaat atgtgcaggt attgcagtaa aacatggtat gaaagaagaa aaagcaatag
    44521 aatcaattac gataaatcca gctaagactt taggaataga agatagagta ggctctatag
    44581 aagttggtaa agatgcagac ctagtaattt gggataattc tccactagag attcaaagta
    44641 atgtacttta cactataata aatggcaaag tagtgtatga gaaaaaataa cttgacaatt
    44701 attaaaaaat taactaatat aagagtgtaa ggaaaattaa aaaatcaaag aagatcttcg
    44761 gggcagggtg aaattcccta ccggcggtat agcccgcgaa ctacttaggt ggttgatttg
    44821 gtgagattcc aaagccgaca gtaaagtctg gatgagagaa gatatttatg gaagataaat
    44881 aagtttattt tagtttgttt aaactatatc cataaaaact cgaagatagg gtcttcgagt
    44941 tttttgtttt tcctaaaaaa tataaacata ttgattaggg ggaaaataat tatgcaaaac
    45001 acagcaaaaa gaggcaatat gatttcaaca agaactttag tagtaatgtc aattttatct
    45061 gcaatatctt atttattaat gtttattcaa gcaccaatac caggtatatt tccagatttc
    45121 ttaaaaattg acttatcaga tattccagca atattcggag gaatgtctct aggaccatta
    45181 gttggatttg gaatagttgt agtaaaaaat gtgttacaag caataacagc aacaacaact
    45241 ggaggaatag gagaatttgc aaatatatta attggtggtt cttatgtaat gatactttgt
    45301 tattcatata agagaagtgg agatttaaaa ggtgtactga ttggttttgt attaggaaca
    45361 atatctatga ctataattgg ttcattagta aattattttg taatgttgcc actttatggt
    45421 caacttatgg gactagatgc tataataggt ttaggttcag ctataaatcc tcaagttcat
    45481 gatttattca cttttgtaat ttggatgata gctccattta atgtgctgaa ggctgttata
    45541 atatctttag taacattgcc actttataaa aaaatgggaa atataataaa gaaataatta
    45601 gacaagaaaa tataaaatta aatgtattga atagccataa tttatttata acttcagtcc
    45661 tatattgtga ttgatatatt aaatatatta tggctttatt aatttatcaa aaaatgtata
    45721 taaaataaga aatatttaga caaatttaaa ttttaaaata aaggaagaat taaaacgatt
    45781 aactattaaa atatgaaaaa ggtatctaca atatacagag cgaatttata ataaacaaaa
    45841 aataaagtta ttattaaaaa actataaaga ctaataatca aacttttaat aataactaat
    45901 ttaataatga taggggtgta tattcatgca tttatctaaa aaaataaaaa aattattttt
    45961 agatgataaa gggtattatt ttaatagtaa ttgggaagat attcatattg gtatttatga
    46021 tacaaaatta ggagattctt taaagtatat cactaatgaa tatattgaaa atatggaaag
    46081 ggtaaaatac gattatcgtg aattaaaaaa tatagaaaat caaactgatg gtatgtgtat
    46141 tttagcaata aggcagaatt acaaggcatt agagtatgta aaaaatcaaa ccgaagaatt
    46201 atgtttagag gcaataaagt ataattataa agcacttgaa tatgtaaaag aacaaacaga
    46261 atacttgtgt cttgaagcta taaaaaaaga ttgtaatgca cttaagtatg tacgaaataa
    46321 aacagaaggg cttattataa aagcaataag tcattcttcc aatatagatg ttgtaagtat
    46381 actaaaggct ttagagactc aaacaagaag gatatgttta gaagcaatta aaaaagatgg
    46441 caggtgtctt gcatacgtta gagaacagag tgaagaatta tgtatagagg ccattaagca
    46501 aaattataaa gcattaaaat atgtaaaaaa tcaaacggaa aaaatgtgta tagaatccgt
    46561 aagacaaaat ggtatggcat tacaatatgt aaataaacaa actgataaaa tatgtataga
    46621 agctgtcaaa caagatggaa gaagtctaca atttgtcaac aataagacag aagaaatatg
    46681 tataaatgct ataagatatt taaataaaaa atataatatt aaagatgtac taagttacat
    46741 agataagtat acagaagata tatgtataga gattgtaaga caaaatggaa aaatgcttat
    46801 gtatataaaa aatcaaacag aaaaaatgtg tattgaagca gtaaaagaga actataagtc
    46861 tcttaagtat gtaaaagagc aatctgagag aatatgtaaa gaagcattga agcaaaacca
    46921 taaagcaaaa gagtatgtaa aaattgctat tgatgattgt atataaggtg tagatacagc
    46981 catcaatagt gaaagataaa aaaagataaa gacaagtact ttataataat ctaggcttat
    47041 actataatta attaagcagt gatataaatt cacatgacga ttatatcaaa atggagatga
    47101 attgatggca aaaatatatt tagaaaatga aaataaaact agagaaatag gatataaatt
    47161 aggtaaactt ttaaaagaag gcagtgttat ctgtttagta ggagatttag gagctggtaa
    47221 aactacaatg acacaaagct tggcagattc attagggata gaagattata taacaagtcc
    47281 tacttttact ataataaatg aatatgaagg aaaaatacct ttgtatcatt ttgatgttta
    47341 tagaataggt agtagtgatg aaatgtatga tattggttat gatgaatatg taaattcaaa
    47401 tggaatctgt ataatagaat gggctaatct aatagaagat atacttccaa aagaatattt
    47461 gaatatagaa ttgagatata aagatgaagg aagagaaatg atattaactc caaaaggaga
    47521 gttttataaa gagatagttg aggagttgat taaataatga aaatactagg tatggatacg
    47581 tcttctatgg cagcaagtgt agcagttgtt gaagatgata acttaatatg tgagtttaca
    47641 gtaaacaaca aaaaaactca ttcacaaaag cttatgccaa tgatagaaaa tatgttgagt
    47701 atgagtgatt taagtataaa agatatggat ttattagctg tatgtatagg tccaggttcg
    47761 tttacaggac ttagaatagg tatggctaca gtaaaagcta tggcccatgt aaataatata
    47821 ccaataatag cagtaaattc tttggaatct ctagcaaata acataaattt ctgtgataga
    47881 aagatatgtt gtatattaga tgcgcagaaa aatcaagttt attcagctaa atacaagttt
    47941 gaaaatggag agatgataga attagatggt gttgatgttg tagagtttga tgctttagta
    48001 gatgaaattg tatcaactaa tgaagaattt ataatagttg gagaagctgt atacaaatat
    48061 aaagacaagt tagagaatat aaaaaatata aaaatacctt caccagctaa taatgtatct
    48121 aacgcaggaa gcttatgctc tttagcatta aataaatata ataaaaatat agatgtacat
    48181 acttgttata cgataaatcc tatgtatatc agaaaatctc aagctgaagt acaatatgat
    48241 gaaaaaatga agaggctaaa taatggaaga taatacaaaa ctaatagtag atgatataaa
    48301 aatagaagaa atgactacag aagatataga tgaagttttt gaagtagaaa aaaattgttt
    48361 tgaagattat tggtcaaaag agtcttttag aaaagagttg tctaatgaag tagcaaggta
    48421 tatagtagct aagttaaatg gaaaaattgt cggatatgtg ggaatttggc ttatacttga
    48481 tgaagggcat ataaataatg tggctgttca tagtgactat agagggaaaa aaatagggga
    48541 caagttgata aaatgcatag ttgatttatg taaagataat aatattgcat ctatgacatt
    48601 agaagttaga gcatcaaata aaatagcaca aaatttatat agaaaatatg gatttaaaat
    48661 gggtggaata agaaaagaat actacagtga taataaagaa gatgcaatta taatgtggaa
    48721 ccaattaaaa gaggtgtaga gtttatgagt gatataataa cattagctat agagagtagt
    48781 tgtgacgaaa cagctgcatc agttttaaaa aatggtagag aagtactttc aaacataatc
    48841 tctactcaaa tcgagacaca taaaaaattt ggtggtgtag ttccagaagt ggcctcaaga
    48901 aaacatgtgg aaaatattga tatagttgtt caagaagctt tagacaaagc aaatataggt
    48961 tttaatgata tagaccatat cgcagttaca tatggacctg ggctagtagg tgctttattg
    49021 gttggtctat cttatgctaa agcacttgca tatactttga atattccact tgtaggagta
    49081 aatcatatag aagggcatct aagtgcaaac tatatagagc ataaggattt aaaacctcct
    49141 tttataacat taatagtatc tggaggtcat actcacttgg ttgaagtaaa agattatggc
    49201 aagtatgaaa ttttaggaaa aacaagagat gatgcatctg gagaagcatt tgataagatt
    49261 tcaagagcta tgaatttagg ctatccagga ggtcctatta ttgataatct ggccaagaat
    49321 ggaaataaac atgctataga atttccaaga gcctatttag aagaagatag ctatgatttt
    49381 agttttagtg gattaaaatc ttctgtatta aattatctaa atggaaaaag aatgaaaaat
    49441 gaagaaatag tagttgaaga tgtagcagca tctttccaag aggctgtagt agaggtttta
    49501 tctaataaag cactaaaagc agtaaaagac aaaggataca atatcataac tctttctggt
    49561 ggggttgctt ctaattcggg gcttagagct aaaataacag agcttgctaa agacaatggt
    49621 attactgtta agtatcctcc tctaatttta tgcacagata atgcagctat gatagggtgt
    49681 gcaggatatt ataattttat aaatgggaaa acgcatgata tgagtttaaa tgcagttcca
    49741 aatttaaaga taaatcagta gagttagatg aatttgtaaa tataaaagta ttgtttcaaa
    49801 aaagtaagta gaagtgaaat taaatataat aaaaataaat atagtatata ttaaaacttg
    49861 aagtaaaact atttataatc tttatattcc aatgaattgc aacaaatttt aggatataca
    49921 gattgtcttt ggcttagatt tgcttcaagt ttaatttttt ctatagaaag attattttaa
    49981 aaagtgggta atataccctt ttttttcatt cttaaaaagt tttatactaa ttatagaaag
    50041 attaaggagg ggaagaaatg agtcaaagta aagaagacaa aataagaagt attttggaag
    50101 caaaaaatat taaatcaaat tttcaaaata aagaaaattt aagtgaattt aatgaaaaaa
    50161 aagcaagtaa aagagcagaa gacttattag atgtatatta taatacatta tctactgctg
    50221 atatggaatt tccatattgg tataatagag aatatagaaa atcagatggt gatataccag
    50281 tagttagaag agctaaagct cttaaagctg ctttttcaca tatgacacca aatataatac
    50341 ctggtgagaa aatagtaatg caaaagacac gccattatag aggttctttt ccaatgccat
    50401 gggtaagtga aagtttcttt gttgctcaag gagaacaaat gagagaagag gcaaagaaat
    50461 tagccagtaa cacagctgat gaacttacaa aatttgggtc tggtggagga aatgttactg
    50521 aaagttttgg aaatgtcgtt tcaatagctg gtaaattcgg aatgagaaaa gaagaagtac
    50581 ctgtacttgt aaaaatggca aaggaatggg ttggaaagtc tgttgaagat ttaggttttc
    50641 actatgaaaa aatgatgcca gattatgatt taaaagaaaa cctaatgagt acacttatat
    50701 gcatgtttga ttcagggtat acacttcctc aaggtagaga agttataaat tatttttatc
    50761 ctttaaatta tggattagat ggaattatag aaatggcaaa agaatgtaaa aaggcagttg
    50821 caggaaatgc atctggagat gggcttattg gtatggaccg tctatatttt tatgaggctg
    50881 ttattcaagt tattgaagga ttacaaactt ggattttaaa ttatgcaaaa catgcaaaat
    50941 atttagaaag tatagagaca gatttagaag ctaaaaaaga atactcagat ttagtggaga
    51001 tattagaaca tattgcacat aaacaacctc gtacatttag ggaagctctt cagttgactt
    51061 atacaattca tatagcatca gtaaatgagg atgctatatc tggtatgtct ataggacgtt
    51121 ttggtcaaat tttatatcct tggtatgaac aagatataga aaaaggacta attacaaaag
    51181 aagaggttat cgaattatta gagctttata gaataaaaat cacttgtatt gactgttttg
    51241 cttcagcggg cgtaaatgga ggagtactat caggtaatac atttaatact ttatctatag
    51301 gtggattaaa agaagatggt tctacaggag ctaatgaact agaagagtta ttattagaag
    51361 caagtatgag atgtagaact cctcaaccta gtttaactat gctatatgat gaaaagcttc
    51421 cagaagattt cttaatgaaa gctgctgaat gtacaaagct tggttctggt tatccagcct
    51481 gggtaaacaa ctcaaatgga acaacattta tgatgaaaca atttgctgat gaaggtatga
    51541 ctgttgaaga agcaagagca tttgcattag gtggatgttt ggaaacgtct ccagggtgtt
    51601 ggaaacagtt aactttaaac ggaaaaacgt actctattgc aggtggagca ggtcaatctg
    51661 ctggctctgg agttcacttt atagctaatc ccaaaatttt agaacttgtt cttatgaatg
    51721 gtaaagacca tcgtatgaat atacaagtat ttgagcctca taataagcca ttagatactt
    51781 atgaagaagt catagaagta tttaaagatt attataaaca agcaataaat gttttagaaa
    51841 gagcaaataa cattgagtta gatatatgga gaaaatttga tacttctata ataaattcac
    51901 tactaaagcc agactgttta gacaaaggtc aacatatagg aaatatggga tatcgttata
    51961 atgcaacatt aaacgtagaa acttgtggta ctgttactat ggtcaattca tttgcagcac
    52021 ttaagaaact tgtatatgat gacaaagcat ttacaataga ggaaatgaag gatgcaatat
    52081 taaataattt tggatttaaa gatgcattag aagtaggaaa ttattctatg gcagaccaag
    52141 taaaagtaga taagactggt aaatatgatg ctatttacaa ggcctgtctt gatgcaccta
    52201 aatatggaaa taatgatttg tatgcagata atattcttaa aaactatgag gtatggttat
    52261 caaaagtatg tgaagaagca caatctttat atgctaagaa gatgtaccct tgccaaatat
    52321 ctgtttctac acatggacca cagggagctg ctaccttggc tactccagat ggtagactaa
    52381 gtggaacaac atattcagat ggttcagtct ctgcatatgc aggaacagat aaaaatggag
    52441 tatatgcatt atttgaatct gcaactattt gggaccaagc agtagttcaa aattctcaga
    52501 tgaatttaaa acttcatcca actactataa aaggacaaca aggaactaaa aaattattag
    52561 atttaacaag aagttattta agaaaaggtg ggttccatat tcaatataat gttgtagatt
    52621 cagaaacatt aaaagatgca caaaaaaatc ctgacaatta tcgtcaatta atggtacgtg
    52681 ttgctgggtt tactcaatat tggtgtgaat taggtaaacc aatacaagat gaagtaattg
    52741 ctagaacaga gtatgaaggg gtgtaaaata tgagaaagca tagtgattgt atgaattttt
    52801 gtgcagtaga tgcaaccaaa ggaatttgta gattatcaaa acaaatgatt aatttagatg
    52861 attcagcatg tccagagata aaagtaatgc caaaatgtaa aaattgtaaa aattttgttg
    52921 aagctaatga tgaaggtata ggcaaatgtg ttggcctaga gaaagaagac tgggtatatt
    52981 caacattgaa tgcaattact tgtgaaggac atgtgtttaa tgagtagtca aaagcaatta
    53041 gaaggcatga ttttcgatgt acaaagcttt tcagttcatg atggtccagg ttgtagaaca
    53101 actgtatttt taaatggatg tccactgagt tgtaaatggt gtgcaaatcc agaaagttgg
    53161 actgtaagac cacatatgat gtttagtgaa ttatcttgtc aatatgaaaa tggatgtact
    53221 gtatgtcatg gtaagtgtaa aaatggtgca ttgagcttta atcttgataa taagccagtt
    53281 atagattgga acatctgtaa ggactgtgaa agttttgaat gcgtcaactc atgttattat
    53341 aatgcgttta aattatgtgc aaaaccttat actgtagatg aacttgtgca ggtaattaaa
    53401 cgtgattcta acaattggag aagtaatgga ggagtaacat ttagtggagg tgagccacta
    53461 cttcagcatg aatttttaca tgaagtatta ttaaagtgtc atgaggtaaa tatacacact
    53521 gccattgaaa caagtgcatg tgtatcaaat gaagttttta ataaaatatt taaagatatt
    53581 gattttgcat ttattgatat aaagcatatg gatagagaaa aacacaaaga acaaacaggt
    53641 gtatataatg atttaatatt agaaaatata tcaaaccttg caaattctga ctggaatgga
    53701 aggttagtac ttagagtacc agttatctct gggtttaatg atagtgctga aaatataagt
    53761 gatattatat cttttatgca caaaaataat ttaatagaga ttaacttatt accatttcat
    53821 aggctaggag aatcaaagtg gattcaatta ggtaaggagt atgagtattc tgataaggga
    53881 gatattgatg aagagcatct tgaagaatta caagatatat ttttggataa tggtatagct
    53941 tgctatgttg gtcatgagac agctttttaa aatcaaatac aagttttttt aattaaaaaa
    54001 ggatgttaca ctaaaagcta gagttacatc cttttttatg tggtttttat gtagaaataa
    54061 ttaagtaaag ttttatatgt tttatatatt gttcatattg ctttatgtcc ataaatatta
    54121 ctttaaattt atactttata tattttttat agtttaaatt acagttagag aatatattac
    54181 gtttacttta ttgaagcaga agttttgtgg taaaatacta ctatgaagaa atttaataga
    54241 atatatttta gaaacaacta aaaataatag ttaggaggaa gacatctata tgaacaaatc
    54301 atggagtaac ttgattatta agaataaaga caaaaaagta cgttcagggt ggattattat
    54361 aactgttatg gctgcctttt acatatttga gtattattta agcactatac ttatggaaat
    54421 aataagaaaa attctgattg ctacaggtga cattaattta aaaacaaact atttttcgga
    54481 atatgctaat tggttaaatg acgttgggtt acaggttgta tttcaaattc taacagattc
    54541 acttatgatt attattccta taattgtatg gagatttatt atgaaaaatc ctattagtga
    54601 aatgggatta cattcttata gaattaagaa aaaagaagga tatgtaggaa tgttattagg
    54661 aatatttaat tgtacagtaa tctttattat aacaataaca attggtggtg gtcatgttac
    54721 ttcatgggta ccaaaaattt cacctttaac agtttcttgg atacttgtct ttattttagt
    54781 tgcttttgca gaagaaattt taaatcgtgg tttttttatg gcagttttac gaagatgtaa
    54841 aaatatttat tttataatga ttgttccatc tgttattttt gggttgattc atatatggaa
    54901 tcctgatgtt acttttttgt ctgtaattaa tattataatt attgggattc ttttttcata
    54961 tatgtttata aagtcgtcga atatatggat gtgtataggt tatcatttta catggaatgt
    55021 atttcagggg attatatatg gtatgcctgt aagtggactt caggttccag ggattataac
    55081 tacacaaatc actcatggta atctattaaa tggagggatg tttggtattg aaggtggaat
    55141 tttgactact tttgttaatc tacttagctt tatatttgta tggtattact atagagattc
    55201 taagtatgat tttgttagtg ataaagtaaa tgtgactaat atcaagaatt agttgattat
    55261 aataaatttt aataatatct tctaaactta aagtattctg taggattgtt ggaaatttac
    55321 aaaaatataa aaataacttg gatattctat ttactccaag ttatttttat attcttttgt
    55381 aaatagaaat ggaacataaa aaattaaaac aaccatagat actattagta tatcatggta
    55441 agtgatagtt gattctttag aatttgtgat aagaaaagca ctattattta aaacatgaat
    55501 tatagcacaa aaccagatat tcttagtttt tatataaaga tatcctaaaa atattgaaat
    55561 tccaactata tgtattgaac gtaatattac tcctaatatt ggcgcttcag gtgtatataa
    55621 tgtaaatatt agtggtaagt gccacaagct ccaacacata cctaatatta tgactcctaa
    55681 ttttttacca aatttatcaa aaaaaatatt ctgtaaaaaa cctctccagg catattcttc
    55741 actaaaaaaa atataaatag tagttaatga tgttattgga gtaaatataa attttatgat
    55801 accactataa ttaggttgag ccccttgtag tattagtata aatgatgttg gaacaattac
    55861 agaaatcata aagtaaataa ttaataaaga acctagttta atatttttta caggatataa
    55921 atttttttta tttgatg
//
LOCUS       DAMKPG010000014        55309 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMKPG010000014 DAMKPG010000000
VERSION     DAMKPG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.016"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            25..1305
                     /locus_tag="PC621_000920"
     CDS             25..1305
                     /locus_tag="PC621_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF4108093.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1568..2959)
                     /locus_tag="PC621_000921"
     CDS             complement(1568..2959)
                     /locus_tag="PC621_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF4108094.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2970..4190)
                     /locus_tag="PC621_000922"
     CDS             complement(2970..4190)
                     /locus_tag="PC621_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF4108095.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4204..4992)
                     /locus_tag="PC621_000923"
     CDS             complement(4204..4992)
                     /locus_tag="PC621_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF4108096.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5009..6283)
                     /gene="larA"
                     /locus_tag="PC621_000924"
     CDS             complement(5009..6283)
                     /gene="larA"
                     /locus_tag="PC621_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF4108097.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6401..7114)
                     /locus_tag="PC621_000925"
     CDS             complement(6401..7114)
                     /locus_tag="PC621_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF4108098.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7239..8498
                     /locus_tag="PC621_000926"
     CDS             7239..8498
                     /locus_tag="PC621_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF4108099.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            8766..9332
                     /locus_tag="PC621_000927"
     CDS             8766..9332
                     /locus_tag="PC621_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF4108100.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9533..10738)
                     /locus_tag="PC621_000928"
     CDS             complement(9533..10738)
                     /locus_tag="PC621_000928"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF4108101.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10841..10947)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11039..11407)
                     /locus_tag="PC621_000929"
     CDS             complement(11039..11407)
                     /locus_tag="PC621_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF4108102.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11459..12736)
                     /gene="obgE"
                     /locus_tag="PC621_000930"
     CDS             complement(11459..12736)
                     /gene="obgE"
                     /locus_tag="PC621_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF4108103.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12847..13137)
                     /gene="rpmA"
                     /locus_tag="PC621_000931"
     CDS             complement(12847..13137)
                     /gene="rpmA"
                     /locus_tag="PC621_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF4108104.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13151..13468)
                     /locus_tag="PC621_000932"
     CDS             complement(13151..13468)
                     /locus_tag="PC621_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF4108105.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13483..13794)
                     /gene="rplU"
                     /locus_tag="PC621_000933"
     CDS             complement(13483..13794)
                     /gene="rplU"
                     /locus_tag="PC621_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF4108106.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13895..15316)
                     /locus_tag="PC621_000934"
     CDS             complement(13895..15316)
                     /locus_tag="PC621_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF4108107.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15334..16035)
                     /locus_tag="PC621_000935"
     CDS             complement(15334..16035)
                     /locus_tag="PC621_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF4108108.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16037..17881)
                     /locus_tag="PC621_000936"
     CDS             complement(16037..17881)
                     /locus_tag="PC621_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF4108109.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18200..19393
                     /locus_tag="PC621_000937"
     CDS             18200..19393
                     /locus_tag="PC621_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF4108110.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19605..20687)
                     /locus_tag="PC621_000938"
     CDS             complement(19605..20687)
                     /locus_tag="PC621_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF4108111.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(21251..22042)
                     /locus_tag="PC621_000939"
     CDS             complement(21251..22042)
                     /locus_tag="PC621_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108112.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22046..22375)
                     /locus_tag="PC621_000940"
     CDS             complement(22046..22375)
                     /locus_tag="PC621_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108113.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(22860..23273)
                     /locus_tag="PC621_000941"
     CDS             complement(22860..23273)
                     /locus_tag="PC621_000941"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF4108114.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(23314..24444)
                     /gene="rodA"
                     /locus_tag="PC621_000942"
     CDS             complement(23314..24444)
                     /gene="rodA"
                     /locus_tag="PC621_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF4108115.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(24529..24813)
                     /gene="minE"
                     /locus_tag="PC621_000943"
     CDS             complement(24529..24813)
                     /gene="minE"
                     /locus_tag="PC621_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF4108116.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(24831..25628)
                     /gene="minD"
                     /locus_tag="PC621_000944"
     CDS             complement(24831..25628)
                     /gene="minD"
                     /locus_tag="PC621_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF4108117.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(25652..26335)
                     /locus_tag="PC621_000945"
     CDS             complement(25652..26335)
                     /locus_tag="PC621_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF4108118.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(26433..29411)
                     /locus_tag="PC621_000946"
     CDS             complement(26433..29411)
                     /locus_tag="PC621_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF4108119.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIVNGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(29423..29911)
                     /gene="mreD"
                     /locus_tag="PC621_000947"
     CDS             complement(29423..29911)
                     /gene="mreD"
                     /locus_tag="PC621_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF4108120.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(29926..30819)
                     /gene="mreC"
                     /locus_tag="PC621_000948"
     CDS             complement(29926..30819)
                     /gene="mreC"
                     /locus_tag="PC621_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF4108121.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(30829..31839)
                     /locus_tag="PC621_000949"
     CDS             complement(30829..31839)
                     /locus_tag="PC621_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF4108122.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(31960..32619)
                     /gene="radC"
                     /locus_tag="PC621_000950"
     CDS             complement(31960..32619)
                     /gene="radC"
                     /locus_tag="PC621_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF4108123.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(32664..33248)
                     /gene="maf"
                     /locus_tag="PC621_000951"
     CDS             complement(32664..33248)
                     /gene="maf"
                     /locus_tag="PC621_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF4108124.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(33468..34439)
                     /locus_tag="PC621_000952"
     CDS             complement(33468..34439)
                     /locus_tag="PC621_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF4108125.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(34455..35030)
                     /gene="rsxA"
                     /locus_tag="PC621_000953"
     CDS             complement(34455..35030)
                     /gene="rsxA"
                     /locus_tag="PC621_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF4108126.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(35045..35638)
                     /locus_tag="PC621_000954"
     CDS             complement(35045..35638)
                     /locus_tag="PC621_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF4108127.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(35651..36220)
                     /locus_tag="PC621_000955"
     CDS             complement(35651..36220)
                     /locus_tag="PC621_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF4108128.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(36220..37197)
                     /locus_tag="PC621_000956"
     CDS             complement(36220..37197)
                     /locus_tag="PC621_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF4108129.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(37220..38557)
                     /gene="rsxC"
                     /locus_tag="PC621_000957"
     CDS             complement(37220..38557)
                     /gene="rsxC"
                     /locus_tag="PC621_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF4108130.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(38773..39717)
                     /locus_tag="PC621_000958"
     CDS             complement(38773..39717)
                     /locus_tag="PC621_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108131.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(39828..41102)
                     /locus_tag="PC621_000959"
     CDS             complement(39828..41102)
                     /locus_tag="PC621_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF4108132.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(41234..41605)
                     /locus_tag="PC621_000960"
     CDS             complement(41234..41605)
                     /locus_tag="PC621_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4108133.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(41833..42432)
                     /locus_tag="PC621_000961"
     CDS             complement(41833..42432)
                     /locus_tag="PC621_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108134.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(42633..42845)
                     /locus_tag="PC621_000962"
     CDS             complement(42633..42845)
                     /locus_tag="PC621_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF4108135.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(42918..44633)
                     /locus_tag="PC621_000963"
     CDS             complement(42918..44633)
                     /locus_tag="PC621_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4108136.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(44633..45187)
                     /locus_tag="PC621_000964"
     CDS             complement(44633..45187)
                     /locus_tag="PC621_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF4108137.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(45180..45782)
                     /locus_tag="PC621_000965"
     CDS             complement(45180..45782)
                     /locus_tag="PC621_000965"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF4108138.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(45791..48439)
                     /gene="polA"
                     /locus_tag="PC621_000966"
     CDS             complement(45791..48439)
                     /gene="polA"
                     /locus_tag="PC621_000966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF4108139.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            48758..48840
                     /locus_tag="PC621_000967"
     tRNA            48758..48840
                     /locus_tag="PC621_000967"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48792..48794,aa:Leu,seq:caa)
     gene            50215..51624
                     /locus_tag="PC621_000968"
     CDS             50215..51624
                     /locus_tag="PC621_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF4108140.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            51640..52842
                     /locus_tag="PC621_000969"
     CDS             51640..52842
                     /locus_tag="PC621_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108141.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
     gene            53043..54026
                     /locus_tag="PC621_000970"
     CDS             53043..54026
                     /locus_tag="PC621_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108142.1"
                     /translation="MKEVFNVHIMQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKS
                     YSKAINNLSKLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDIN
                     FLDIDDYFYRSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLK
                     RYSTRYVNDKVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENII
                     KAKLKDKREWDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFK
                     ELEILDPRYIKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
     gene            complement(54476..54919)
                     /locus_tag="PC621_000971"
     CDS             complement(54476..54919)
                     /locus_tag="PC621_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108143.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(55078..>55309)
                     /locus_tag="PC621_000972"
     CDS             complement(55078..>55309)
                     /locus_tag="PC621_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4108144.1"
                     /translation="QLKYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     ICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT        17693 a         9881 c         6278 g        21457 t
ORIGIN      
        1 aattaaagat aaaaggagta ttttatgaac atacttggat taatagttga atataaccca
       61 tttcataatg gacacatata tcatttatct aaatctatag aaaaaacaaa tgcaacacat
      121 actgtagcaa taatgagtgg aaattttcta caaagaggcg aaccagcttt atttgataaa
      181 tatactagag caaaaattgc tgtcgaaaat ggtgtagact tagttataga attaccaact
      241 ttatttgctt gtcaaagtgc tgaaattttt tctcatggtg ctataacaat tttaaattca
      301 ctaaactgca taaattctat atgttttggt agtgaagaag gaaatacaga aattctttat
      361 aatatagctg aaattttagc aaatgaacca aatgaattta agttactttt aaaaaaatat
      421 ttagatgatg gcctgttatt tccaacagct agagctaatg cactttttga ttatatacat
      481 caagacaaaa aaaataattt tatacaaaat tattcaaagg aacaattatc aaaaacatta
      541 aattcatcaa acaacatact gggtatagaa tacataaaaa acctcatcca tttaaacagt
      601 agtataaaac catttactat ctccagggtc caatcagaat ataattccga agaaattgag
      661 agtgatattt gttctgctac tgctataagg acttcactta aagaaaatac agaccttttt
      721 catttaaaaa atgttattcc agaaaaaact tataacatcc taaatgaaaa agtagataat
      781 ggttttttac ctatgtttga tgacttcttc tttgatattt taaaaacaat tatacttaga
      841 gatatggaca ctttaaaaga gtattttgaa gtaaatgaag gcatagaaaa cagaatctat
      901 aaagatatct ttacatctga atttctacat gagctacaat tagcaattaa atccaagcga
      961 tacactttaa ccaaaatcaa acgaactcta aacaatatat tgttaggtat taaaaaaagc
     1021 gacatgcaac ttataaaaaa catagatact ataccttata taagaatact tgcttttaat
     1081 gataaaggta gagaaattct aaaagagata aaaaataaat ctgaaataaa aataataaac
     1141 aagttttcaa atatatcatt ttcattagat gatacgattt ttaaaacact cataagttat
     1201 gatataaaag ctacaaatat atataattca atatattata aaaacaataa agatttagta
     1261 aaaggcccta tggattttta tactcatcca atatatgtaa agtagttttt taaaataact
     1321 atatttataa aaatcatttt attaactctt tattatataa gatacaatag tcttacttta
     1381 tttatgtaca catgctttta ggatgacaac atcaacttaa atattatgtt actttctaaa
     1441 tttaagtaaa tattgccatc ctaaaaattt tacaatttag cttcagattt aaaagtaaag
     1501 tttaaaatat tatatttaaa tctaatttaa tattttttgt tttgtaaata ttattaaatt
     1561 aatatttcta atatattttc cctggattta aaatattttt agggtcaaaa gctaacttta
     1621 tatttttaat taacatcata taagcatctg aatttgattc atgtaagtac tcttttttag
     1681 catatcctat gccatgttca ccagaaactt gaccactaag ttctctagat tttttataca
     1741 tatagtcaaa agtttctttt aatcttattt tccatgtatt atcatccata ccatctttta
     1801 aaatatatat gtgtagattt ccatctcctg catgaccaaa actttttatt cttattttta
     1861 atttgtcttg aagctcgtga gtgtatctta taaactctgc tatcttatct cttggaacaa
     1921 cgacatcaca ttcatccatt tgtgtcgtag atgcctttat agcttctaaa aatgcacctc
     1981 tggctgacca tatagaatcg tttctttctt gtgtatctga tataaataca tctaatgcac
     2041 cattttctaa acataaatta gctacttttt catattcctt ttctatatct tcagtgctat
     2101 ttccatcaaa tgtaagtaaa agataagcat ctgatgtatt atctggaaac tttttaccta
     2161 aaaattcttc tgctgccaat attacatctc tttccataaa ttctatagcc gttggtattg
     2221 attttgattt tattatttta ggaacagttt ctattgccat tgataaatct ggaaatggta
     2281 taagcaaact tatacttttc tttggtaaag gaagcaattt taatatagct ttagtaacta
     2341 ttcctaaagt accttctgaa cctactaata aatcctttat actatatcca gaactatttt
     2401 taaccacctt tccacctaca tttataattt ctccatttgg tagtacaact tcaagtcctc
     2461 ttacataatc ccttgtaacg ccatatttta ctgctctcat accacctgca ttagtgttta
     2521 tattacctgc aattgtagca cttttttcac ctgggtcagg tgggtaaaat aaatctctat
     2581 cttgaacata ttgacctatt gtcatcaata aaacacctgg ttctactgtc aatgttaaat
     2641 tttcataatc aacttcaagt attttattca ttttacatag attaatcatt attccaccat
     2701 ttatggcaac agcagcacct acaagaccag taccttgacc tcttggtgtg actggtatat
     2761 tatttttata tgcgtatttc attattttag atacttgttc agtttcaagt acatttacta
     2821 aaacttcagg gtacttctca atccctccaa gttcatcatg tgaaaaatct tcatttatat
     2881 cacttccaac taatacattt tcttctccac aagtatctat taaaaaatct atatcttttt
     2941 tatcgattaa tttatacatt tctatccccc tatttagctg ctgatgccat atatttttga
     3001 ttattatctt tgttattttc tattttttca atcaatttag gaataatatc ataaatatca
     3061 cctattaaag ctaaatgtgc tacatcaagt ataggcgctt tatcatcttt atttattgct
     3121 actatatagt cagcaccctg catccctgca acaaattgaa ctgctccaga tacaccacaa
     3181 gttattatca atttaggttt tacagttcta ccactaagac ctatttgttt tttagcatct
     3241 atccaacctg cttctattac aggtcttgtt cctgccactt gagcgtttag cttatcagct
     3301 aatttatata tcatctccat atcttcttgt ttttttattg ctctgcttgc cacaactatc
     3361 acttcagctt cttccagacc aacttcttta tttttttctt ttacactcag taccttaatt
     3421 ttagatttta gttctttttt ttctacttta cataaagtta tctttcctgt agtattctct
     3481 attttttctg gtgctgaaaa tattttatat cttacagtgg caaattgtgg tctattatta
     3541 ggtgtgttta tatgagccat tatatttcca ccaaatgctg gtcttatttg gtctaaatca
     3601 gtatttgatt gaatatcaag tattgtacaa tctgctgtaa gacctgttct aaatctagcg
     3661 gcaagtcttg gagctaatga cctacctaat gtagttccac caactaatac tatcgttggt
     3721 tttattttat ctataaagtt ttctattgct tttgaatatg gttctatcct aaaatcttta
     3781 aattcatctt catcatatac aaatacttca tccaccccat aagaaatcaa attactgcac
     3841 ttatctttta tgtcttttcc tacaaataca caatacacag gttgttttat ttttgatgcc
     3901 atctcacgag ctttacctat aagctcgtac gttacaggat gtatatttcc attatgatgt
     3961 tcaacatata ctgcaattcc cctccattca tctttattaa tttgtacttt actactctct
     4021 ataaattcaa atgcgccttt tggtccactt ttgacacaca acttgcacat cttacagcca
     4081 gcacttattt ctactatacc tgacttttct tctatagccc caaaaggaca tatttctaaa
     4141 attctcttta agtcagttat tttatcttga tttacaacta tctttgacat ttattactcc
     4201 cccttacacc aactttaatt cctctaattt atttacaagt gcaaaactta gttcatctgc
     4261 atttcctctt acaatatttt tatctgtatt tggctttgga ggaaatattc ttacaacttg
     4321 agtaggagaa ccgttaagtc catagttatt ttcatcttta tcttcaaagt catctaagct
     4381 atatacaggt atttctctat ctttagtatt aagttttaat ttaaatgaag gtagtcttgg
     4441 ttgaaaaatg tctttttcta cagttattaa acatggatat tcaatctcac atacttgtaa
     4501 atcatttggc atatctattt caacaactat actttcatct ttaacttcaa ttaattttgt
     4561 aacgttagta acatgaggta tatctaaaaa ttcagctact tcaggtccca cctgtgctgt
     4621 atcaccatca gtagtttgtt ttccacaaat tattaggtct acctttccca tcttcttaat
     4681 accttgtgat atggtatagg aagtagccaa tacatctgct cctccaaatc tcctatcact
     4741 tattaatgct ccttcatcag ctcccatcat aaaactttct tcaattacct tctttgcctg
     4801 aggaggaccc atgctaagta cttttagatt cgctttctta tcttccttta ttctaagagc
     4861 tgtttctaaa gcatataggt catagggatt catttttgaa tctactccat ctctttttag
     4921 tactcctgta gtttcatcaa cttctactga agtcgttcca ggaacttgtt taatacatgt
     4981 caaaatttcc ataaagtaca ctcctttttt atttaactat aactgatact ccatcaggta
     5041 taactactac tttgctctcc ttaccaacta tactattcgc catttctaaa gcttctttaa
     5101 agttaaatgc atgtttcata tgcatttttt ttatcatttc aggattacac ttatctgtta
     5161 ccattatcac agtatattta gataatattc tcgctagtat ctgaaattcc cattggtctg
     5221 gaatcgtatc tactctatcg acacttctaa ttttatctaa aagttgtttt ggagaatctg
     5281 catttgctac attttcataa aaagattgac ctccatgacc atcattacat gctgaaatca
     5341 ttattattac tccaccgtct ttacaagtag cttcagctgc tgtcattcct ttaacagatt
     5401 gataaatatt ttggtctagt ggatatccac catttgtagt tacaacaata tcagatttta
     5461 tagaatttac ttttgataac tctgttacaa atctacatcc tttagtatgt gctttttcga
     5521 catcaccagc aaaggaagca attatttttt tatctttatc tattacaaca ttcaatatga
     5581 atgctaactt tgcaacttta gctgcatata ccatatcctc atgaatagga ttattattca
     5641 atttcccagt tcttgaatta tcactatcta taaagtcact acagtgattg tacattatag
     5701 tctttgcaga tgcgattcct ggaagtacgc tttttctgcc acctgaaaaa cctgcaaaga
     5761 aatgaggttc tataaaacct tctgctatca aaagttctgc ctcatatgct agtttgttta
     5821 tatataaatc tccacctgaa ggcaatatcc ctactttaca catagattta tcatcagtag
     5881 atatatgcat aactatatct tcattatcta ctatttcttc tcccatttta taaattagct
     5941 cttcccttgt acttggccta tgaaatccag ttgccactat aatcttcaca tcaatttctg
     6001 gattaacatt cctaatcctt cttaatataa ttggcatagt tattctactt ggtacaggtc
     6061 ttgtatggtc acttgtaatt atgactacat tctttttccc cttaaccagt tcttccagag
     6121 aattagagcc tataggatta ttcatagatt cttcaactat ctcttcttga gtcttatgtt
     6181 ctatataatt atccgatttt gactccaaaa caccgagaaa acgttcatca ggtatttcta
     6241 aaatcattcc ttgttttgaa tacggtactt ttaactttac catttttaat ctcccattat
     6301 aataaactat aaaattggta tgtggtcata ccagtttcac atttatatga tagtaccatt
     6361 atctaccatt tgcaagaata atttatttta taacaaactt ttaattttca ttttttctat
     6421 aatttttact tataaaatcc atatgttcct ttgctttctc acatgcaagt tctacatcat
     6481 tttctacaac tgcattgtat atagcttcat gctgtttaag caatatattt tcattaaaag
     6541 gttctgcgat tattttttct cttgcatctt ctataaatcc atcaaataat tttgatgctg
     6601 tcataaaaag actttgaatt aaataatttt tacttaatgt tgctagttta ctgtgaaatt
     6661 tttggtctaa taacaccagt actcgattat tatctttttt attagtttct actctcattt
     6721 catctattat atgtttcaat tctataactt cagaagaagt tattctcttt gaagcaagtt
     6781 ttgcaatctc aatttctagg attattctaa gctctaatat atcttcaagt gtgccattat
     6841 taagtttaaa aatcatagat aaaggttcta ttaaagtttt ttctatattt gtacatataa
     6901 aatttccttc accctgtctg ctttctatca ctcccatagt ctctaatacc ctaatagctt
     6961 cccttatgga agttctactc acattcattt gttcagacaa ttctctttct gatagtaatt
     7021 tatccccctt ttttaactct ccatttagta ttttatattg tatttgctca attacttgtt
     7081 cataaacttt tttactagct atactattaa acattttttc ctccaatatg ataatcgttg
     7141 ttgtaatata tatctattat atatcttaaa ccattacatt catatatttt tattactttt
     7201 aatataatat cttataactc aaatttacag gtgatattat gaaaaaaaat aaaattattg
     7261 ctttttttgc tccagctatt atagttggag ttctagtttt agggattatc ctatacccaa
     7321 atgaagctat aaaatctgca agagaaggct tctctatatg gtctaatgtt ttagttccat
     7381 cactactacc atttataatt ggagcaaact taattgttga tttaaaaata gtggatatta
     7441 tagggtttat catcaatccc ataactagat ttgtatttaa tgtttcagga aagagcgctt
     7501 tagcttttgc tatatctaca gtatctggtt atccagtagg agtaagttta gcttctgaat
     7561 ttaggagtaa tgggcaaatt tctaagcatg aagcacaaag acttgtgtct ttttgttcta
     7621 catcaggacc tttattcata ataggttcgg ttggtacagg tatgttccaa aattcaaact
     7681 taggttattt aatgattctc tgccattatc taggaactat attggttggt cttttcttta
     7741 gaaattatgg taatgaaaat cttccaaaga caaaattgga tttaaaaaca aatataaaaa
     7801 atgtaatcaa cattagaaat tcaagtggga atggtttttt tgttttattt ggaaatgctg
     7861 tatttaatgg agtaaataca ttacttacag ttggtggatt tgtaattgtt ttttcagtag
     7921 tatttaaaat actatctctt tttaatgtaa tctcattaat agcatcagtt atatatttgc
     7981 ctctttcatt tttaggtgtt agtaaagaac tctgtcaagc atttgtaagt ggtctatttg
     8041 aaattactat aggatgcaat aaagtatcta gtataacaag ttctccagaa attttaagag
     8101 catctttagc tagctttttg ataggattta gtggattatc catcctagct caatgttgta
     8161 catttctatc aaaaacggac atagatttaa aattatacat actaagtaaa tttgcgcatg
     8221 gagttttagc tgctatcttt atatttatac tttatccaat tgcaaactct gctgtgttgg
     8281 tttctagctt tgctgataca tacaacgtta tatataataa tttaatctgg ttttattatc
     8341 ttagctatta tgatacaatt cttcaaattg tagtaataat ttatctaatc tcagcagtat
     8401 ttattgcaaa aaaacatatg aaaactttat ctacaaatgg gaaactaatg cgatataaaa
     8461 atactttttt tattaagaaa ttttcacgaa aaaaataaaa ctaataaaac ttaactaaat
     8521 acttccattt ttttattata tttgtcataa ttttaataac ctttttaatt agtttaacag
     8581 catagttatt tcatcacaat ttaataattt tattagaaat atttttcaaa aaattctcct
     8641 taatcacata tatcacactt gtataagttt actgggaaat ttttccatta tataatgttt
     8701 catttatatt atataataaa taccgaccta aacttaccaa atcaaactaa gaggagtggt
     8761 tgaaaatggc aacaagacct atagaaatag acgaatataa aaagataatg gaactattac
     8821 atactggctt tacttatagt gaaaatggcg ttgaaaaaag atttagaaaa aatcctaaag
     8881 ttgcattggc actaatgtta gaagctaatt taggtctaag aatatcagat attttaagat
     8941 taaagatagg taattttaaa ggtaatatgt tggaaataaa tgaaaaaaag acgggcaaat
     9001 tacagtatag accagttaat aaaaatataa ttgagtccat aaatgaacat gcaagaaagt
     9061 ataatcttaa atcaaatgac tacttagtaa atataaagac aaaggctatt caaaaacagt
     9121 tgaggataat ttgtaaacac ttaaatctgt ataatataag tacacatagc tttagaaagc
     9181 tctatgctac tactcaattt gaaaagagta acaataattt agagttggtt aaagaacttt
     9241 taaatcacag ttctgtagcc actacacagc gatatataag agtaactcag caagctatta
     9301 atgaagctag tgaaaatttc tttattggtt aattataacg tatacatttt tctattatct
     9361 tattaattca ccagatatta aaaaagagtg tctcaaaatg aactttttag ttcatgagac
     9421 actctttttt tataaaatca aatatatttt tattatttca atactggaaa aaaggcttgt
     9481 ctctctattt tgagacaccc tattaaattt tcataattct tgcataaaca tattaatcta
     9541 tcttcaataa ttttttagct gattcactgt aattatttct ttttacaatt tccaaaactc
     9601 tatcaatctc atgttctgac tttgcactta caaaaatcgc caattcttca tcatctataa
     9661 tatcataact atctacatat ttatctaaac attctaaaat tccatcttta ttattatacc
     9721 caccaaatct aactactgca ctacactcat cttctataag tccacttaaa tccaatttcc
     9781 cttttgaaaa agactttata tttgaacttc tattatctat tatatctata atatctgcat
     9841 aaacagcact tcctgttgca cctcttccag ctcccttacc tacaaaagaa agttcaccta
     9901 ctgaatcacc tactaaaata actacgttga actcattatc tatctttgaa agcatattat
     9961 tattgtccac taatgctggt ctaacaaatc cacttacttc tcctgattca tacttacttc
    10021 tagcaaccaa ttttatctta caatttaatt tttttgcata ttctatatct ttttcatcta
    10081 ttgaagttat tccttctaat aataaatctt tccaatatac tatttttcca taagataaag
    10141 tagacaatat agataacttt ctagcactat catatcctaa aacatctgca tctggattaa
    10201 cttctgcaaa tcccaactct tgtgcctgtt ttaaagcaac atcatatggt aaattttcat
    10261 catacatctt tgataatata aaattacaag tcccatttaa aatagcgttt atgctcttta
    10321 tattgttgcc ttccagtgat tctatcaatg gttttaaaac tggaatacca cctgccacag
    10381 atgcttcaaa cttgatgcta acattattta agttagctaa ttctataagg tcactacctt
    10441 tttcagccag taaatcttta ttagctgtca ctacatgtat ttttttattt aacgctcttg
    10501 ttacataatc aaaagctgga tttaatcctc caattacttc aactagtata tcaatatcct
    10561 tgtcaaatat atcttctata tcagtagtta tcttatcaaa atacttctta tacttgtgtt
    10621 tttctttatt tttaactaaa attccaacaa cttctatatc tctttttgaa gtattatttt
    10681 ctattaaatc aattaatcca cttcctactg ttccataccc taatattgct atcttcactg
    10741 taacataccc ccatcatttt gattatatta aattatttat atatataaat ataccaataa
    10801 aaaaagcttg agtataaact caagccatat gacgggttta tactcatctt tcagcataaa
    10861 tgctgcagga tttagcacca atgtataatt aaatatactg gttgccgggc ttcacagggc
    10921 cagtccctct gccactcttg ataagattgc taattaaaat ttatgtttaa tagtataata
    10981 caatttcttc caattgtcac tattttttta acttatttcc ctaaaaggat ataaactatt
    11041 atctaacagc tcttttagtt ggtttaccct tttttgtaac attattattt ttagtctttg
    11101 ctttcgcctg ttcatgtcct ggaaaaagta ttttaggctc ttcaatattc ctcttgtaaa
    11161 tagtaaattt aaaaccaata gcctgcacaa attcagctct taaagcttca catactgcat
    11221 tagcagtttc tttagtatca agacctgcat tttctaatat agtaactttt actaattctc
    11281 tagttcttaa cataccatct aattgctcta agaaactctc tgtaacacct tctttaccaa
    11341 tttgtgtagt aggctttaaa gtatttgcta aagatcttaa atacgccctt tgttttcctt
    11401 ttaacatatt ctcacctcta taattctata cttaaattat ctataatcta tatttaaatt
    11461 aattgtaaaa ctcaaattct aattcataaa ttttaaccac atctccatct tctattccca
    11521 tttctctaag cctatcaaaa acaccttgac tctccatagc tttttggaaa tattgaaggg
    11581 attccatatc atcaaaattt acagaataca ttattcttct aagtgcttta cctgtaacta
    11641 catacacacc atcttcaata tctatactta actcttcttc agtaccaata tcaagttctg
    11701 gtctatacat ttcttcttct gaaactagct ccacatcttc tacttctttt agcattttag
    11761 aaacataggc tattacttca tctatgcctt gacgagttgc tgctgacatt ttaaatactg
    11821 tatatcctct gccttctaat tcactcttaa acttctcaaa cttactctca tcttctaata
    11881 tatcaaattt atttgcaact acaacttgtg gcctctttga aagcttttca ttgtacaatt
    11941 taagctcatc atttatcttg tcaaaatctt caatagggtc tcttccctct aatccagata
    12001 tatctacaat atgtatcaat acctttgttc tctcaacatg tcttaagaac tcatgaccta
    12061 atcctatgcc ttcagaagca ccctctataa ttccaggtat atctgcaagt acaaaactat
    12121 ctccaaactt agtttgaaca acacctagat ttggtgtaag tgttgtaaaa tgatagtttg
    12181 caattttggg tttagcttta gttacaactg atagaaatgt tgatttacct acatttggaa
    12241 atcctaaaag accaacatct gctatcattt taagttcaag agttatccat ctttcttctc
    12301 catctgtacc agattttgca aaagctggag cttgtcttac tgcatttgca aagtgttggt
    12361 tacctttacc acctatacca ccttttgcaa ctatagctgt atctccttct ttttttaaat
    12421 ctgctaatac aagacctgta gcctcatctc ttattactgt acctgctggt acttttaata
    12481 ctaaattttc tccattttta ccagctcttt ttcctttgct tccgtcgcca ccattttgag
    12541 cttgatattt tttctggtac ttaaagtcca ttagagttct aagccccaaa tcaacttcaa
    12601 atatgatgct tgctccacga ccaccatctc caccgtctgg accaccagct ggaacgtatt
    12661 tttctcttct aaatgctacc gaaccatttc caccatttcc agccttaaca aatattcttg
    12721 ccttatctat aaacaaattc ttcacctact taattaaaaa cttttaatta gcattaccaa
    12781 aaataccatt ataaattcat cagtattata agaaaaagga gtgcctcaac actcctttta
    12841 tgtatattac tcagctattg ctactggata tatactaact ttttttctct tcttgtcttt
    12901 tctttcgaat ttaacagtac catcaactaa tgcaaataaa gtatcatcac taccttttcc
    12961 aacattagtt cctggatgta ttttagttcc tctttgtcta actattatac ttccagcagt
    13021 tactaattga ccatcgaatc tcttaacacc taatctttta gatatagaat ctctaccatt
    13081 tttagaagaa cctacccctt ttttagatgc taataattgt aaattcatgt ttaacatatg
    13141 gagtcacccc ctactttttt aaaattttta tattttttgg gtattcccgt ttaatttctt
    13201 taaccgctaa ttgaaagtga tttagtaata attgtgcgtc ttttacatgc gtatctttga
    13261 ctatacactc tatatgacct tcttctccta ctactttttc aaattcaatc ttttgtaatt
    13321 tgtctaaaga atttataact gcaatagcat tagatgtaac tgcagcacat actatatcat
    13381 agcctatttc agcaaaatca gcatgtccct ttaaacaaaa accttgcaac aagaaatcat
    13441 cattataata atatttaact tttatcataa cttaaccaat aactaagcgt ttattttttc
    13501 tacaactatc ttagtgtatg attgacgatg tccttgtttt cttctgtaat ctttttttgc
    13561 tttatacttg aaaactataa ctttcttagc tttaccttgt tcaacaactt tagcttgaac
    13621 agatgctcct tctactactg gagaacctaa ttttaattct ccatctttag agcaagctaa
    13681 tacttcgttt aaagtaacaa catcacctgc gttagcttct aatttttcaa cgaataatac
    13741 atcaccttca gaaactttat attgctttcc accagtcttt actatagcgt acattaaata
    13801 cacctcctcg gcctcaaact cgccagtaca aggtacttca tttgaagatt taaaccttta
    13861 ctgtgcggca ttacaattat taattataaa atatttacat tttattgtca actattttat
    13921 cgaaaataac attggtttta ttgtgcttta atgtactatt tgcgtttaaa gaaatcttta
    13981 tattatattt ttcaccaatt ttatcaatta tatccatata atattctttt atttgttcaa
    14041 aaataaaatc attaaattct accattatgt ttttgtaaac agtatgttca cttattctca
    14101 taatttcttt ttcaatacta tctagtatat aatgtacaga ttttattgtt tgttcaccat
    14161 cacaacatgg acaagacatt aaataatact tatcaatgga ttctttttct ctacgcctag
    14221 caacttctac taaaccaagt tttgtcatac ctaatacttc agcttttctt ttatctttat
    14281 taaattcttc ttctaggatg tgtaaaatct ccttttgatg cttcttttta tgcatatcaa
    14341 taaaatcgat tattattatc ccagctatgt ctctaattat aagctgtctt acaatttctt
    14401 ttgcagcttc aagattagtt ttataaacgg tttcatcaag tttaccagtt ccaataaact
    14461 tacccgtatt tacatctata accgtaagag cttctgtttt ttcaattatt aaataacctc
    14521 cactctttaa ccatactttt ttatttaata atttttctat tttagattct attctgtata
    14581 aatcaaatac atctctattt tcctcaagac acagtttatc aacataagtc ttatctatac
    14641 ctcttaaaat agacttaagt tcagagtact tatcataact attggtaatt atagattcaa
    14701 tatcatcatt cacattatct tttacatatt tagttgcaaa atctaaagac ttatataata
    14761 atttcggacc tataccaagt ttatattctt ttaaaatatt ttcatacttt aatttcaact
    14821 cttctatatc tttctttatt tcatcatgag tgcaaccttg agcctcagtc cttattatta
    14881 atcctagatt ttccttattt actgccttag taattttttt taatctaaat cgttcttttt
    14941 catcagtaat tttatttgat attgtagttc tatcattgga aggtatatag acaatatacc
    15001 ttccagaaat actaatctca gtgtttaatt ttgcgccttt agttcctatt tcttctttat
    15061 ttatctgaac caaaatatcc tgaccagatt ttatagtact tccctttttt aattgtaaat
    15121 aagcaagttt cttaaatcca atatctacaa aacaagcttc tattcctggt ataacttttt
    15181 ttacgattcc acgatatata ttggatacag tcttcttatt taaattgtct tcaacaaaaa
    15241 gttctgttaa tctttcatct tcaagtactg ctgttttctg ggaccctatt agggactcaa
    15301 taactatctt tttcacaact tcacctctcg agcttacata ggagtcacta attccccatc
    15361 ttctaataca tataaatctc ttctcaatat atcaacatct aatgggtcaa tatcaagttc
    15421 aaacatctct aatatctttg gtataagtat attagtattt aaattagttt ttgagcctgt
    15481 agctatagta gcagtaagag taatatgttc atcttctaaa tttaaaacat caaaggttct
    15541 tatcataggt cttatatcta cttcaaccat cttacctttt ttattctttt ttgttatgat
    15601 tatttcttta ttattcataa agtcaattac ttttcttttt acaaactctt tcgtcatagg
    15661 tcttttcaaa tctatattaa acaaatattc tccatagtct atcacagaag aaagagaagg
    15721 tgtttgtcta tcaatttctt ttgctttgat aaaatctatc ccatctggaa gttgaattga
    15781 tactttattt aaaaattcct ctactgataa atcatcttct tctatttcaa tatctacata
    15841 ctcaccttga ctttctgtcc ctaaagccaa agcattgcca taactcattt tagggtgagg
    15901 attaaatcct tgagaatgtg ataagtttat ttcagctctt ctaaacgctc tttgtaacaa
    15961 tctctgaagg tcaagatgtg atatatatat catgtcgcct tcctttttaa attttactct
    16021 tattatctta ctcatattag caaagccccc ttcctatatc atgtatattt attccacaac
    16081 cattgcagtt atgtctacaa tcagaagtga ttttttcttt tttagcattt tcgtcttctc
    16141 taattaagaa ttttttacta atacctacat ctatatggtc ccaaggaaat acctcttcat
    16201 agtttctatt tctatgtgca taaaaatcta tagaaagatt attcttttcc atagcttctt
    16261 tccatatatc taaattaaaa tgttctgccc atccatcaaa tttagcacct agtctaaatg
    16321 catcatatat aacttttcct attcttctat cgcctctagc tactactgct tccatcaaac
    16381 tagtctttga atcatgataa ttgtatttta tattgttatt tcttaattta tttactaaat
    16441 gtctctgctt attaataact tcttctgtag tatcttgacc atgccattgg aaaggtgtaa
    16501 atggctttgg tacaaatgta gaagtactca ctgtaacact aaaacttctc tttagctttc
    16561 ctccatttac ttttctataa atatcaataa ctttataagc taatttagct ataccatcta
    16621 aatcttcata agtctcagtt ggtaaaccaa tcataaaata caacttaaca ctattccacc
    16681 ccatctcaaa agctctttct gttgcatttt ccaaatcttc ttctgtaaca cctttattta
    16741 taacatctct aagtctttga gttcctgctt ctggagcaaa agtaagtcca gattttctta
    16801 cttgttgtat tttatcagca atttccattg aaaaattatc taatctaagg gatggaagcg
    16861 aaatcccaat attattagat gcatattcat ctaccaaata atcagtcaac tctgataact
    16921 tactataatc actcgtacta agtgatgata atgatatttc atcatatcca gtattcttaa
    16981 ctaatttatc taatatttct ttaagtcttt taacactctt ttctcttatt ggcctgtata
    17041 tcatacctgc ttgacagaat ctacatcctc tagtacatcc tctaaataat tctagtacta
    17101 ttctatcatg aacagtatct atataaggta ctattagttt ttctggataa tctacagtct
    17161 caacatcttt tattatcctt ttagtaggat ttttagtaat cccttctcta ttaggagtta
    17221 cactttgaac tgtaccatct tcattgtact tgacatcata aaaacttggt atgtatactc
    17281 cttctatgct agatatttta tataaaaaat cttctctagt cgttttattt ttcttccatt
    17341 ctttatactc atttactact tctaaattaa cctcttcacc ttcacctaat attactacat
    17401 ctataaaatc tgctaatggt tcggaattat aagcacaagg accaccaacc attataaatg
    17461 ggtcttccaa agttctttct tcttttaata ttggtatatt ggctaaatct aatatattta
    17521 agatattagt gtatgaaagt tcatattgaa gtgtaaaagt gacaaaatca aagtttgtta
    17581 taggctctct tgactctaat gcaaataatg gtatactttt ttctctcatt atattttcca
    17641 tatcaactgc aggagaatac actctctcac aaaatacatc ttcatcctta tttattacat
    17701 catataatat atgactccct aagtgactca taccaacttc atataaatct ggaaagcaat
    17761 gtgcatatct tatcaattca ctattactag tatctttatg aattgaattt atttcatttc
    17821 caagatatct agcaggcttt tctacttttt ttaagatttt tttcaagtct actttattca
    17881 tttattaaaa ttcctccaaa caagctcttt atttttattc ttatttataa gtagtattat
    17941 acacgataaa atattttcta tcaaaaatat aactaaaata ttgaatttaa aatacatatt
    18001 attcacaaaa tagaatagct aaaaatattt aagaatattt tctattaaat ttttattgaa
    18061 atacaatttt tttacaaagt atgatatatt ataaacataa aactattgtt ttacatgact
    18121 tatcatgaca tcttttgttt cattcacctc aaaattgggt atgttactta tataatgata
    18181 aattttagga gggttaaaaa tgagtaaagt ttttgaagta aaaaaagata tctactttac
    18241 tggcgtagta gatgaaggtt taaaagtatt cgacataata atggaaactg agtttgggac
    18301 aacttacaac tcttatttaa tcaaggacga aaaaacggtt ttatttgaca ctgtaaaggc
    18361 aaattttaaa gatgagtttt taagtaattt atcagaggtt actgatatag ctaaaattga
    18421 ctatgttgta attcatcaca cagaaccaga ccatgcaggt agtttaaaat acttattaga
    18481 tataaatcct aatatagaag tttattgtac taaagctgct aaactatact tagatggtca
    18541 aataaataga ccttttaact gtcatgtaat aaaagatggt gaaattttaa atatcggtaa
    18601 aagaaatctt agatttataa ctgctccatt cttacattgg gttgatacaa tgtttactta
    18661 cattgaggag gacaagactc ttttaacttg tgatgcattt ggttgtcact ttgcaagtgt
    18721 tgatgcagag gtagtgaata gtgaagatta tctaaaatca gctaaacact actatgattg
    18781 tatagttaag ccatttgcta aacatgtttt aagtgctgta gataaggtgg taggactaaa
    18841 catagaattt gatactatat tgacttctca tggtccaatg cttactaaag acccaatggc
    18901 tgctgttaaa agatatgttg agtggtctac agaagctgtt aatacaacta atcaaaatca
    18961 agtttctata ttctacttat cagcttatag taatacttta gagatggcta aaaagataaa
    19021 agaaggactt gataaagaag gtgcaaaggc tgaattatat gatttagaag atatgacatt
    19081 aacagaaatg cacgatacat tagtagtgtc aaaagtaata ttactaggtt ctccaactat
    19141 aaacaaaact atggttaaac ctatgtggga tttattctct gttatagacc caatggctaa
    19201 ccaaggcaag atagcaggag tatttggttc atttggttgg agtggtgaag gtataactat
    19261 ggctgagact ttacttaaat ctatgtcatt caaaatgcct gtagaatctc ttaagaaaaa
    19321 attcttccct agtgaagaga cattaaaaga gtgtatggca tttggtgcag aatttgcaaa
    19381 attagttaaa tagccattaa tcacacataa tattatttaa aataaatgat tttatattta
    19441 aaaataaatt aaaatggagt tgtctcaaaa ttaatttttc taatctaatt ttaagttcaa
    19501 ctcctttttt atttcccaaa atgactaata taatgataaa tgcaccctca ccaagagggt
    19561 acattattta taatcttttc tatttgtata cttgtatatt tatattaatt cacatatcta
    19621 gcatatctta cttgccctct tccagaatta ggttctattt caccaatttc tattttaata
    19681 attacattat tgtttttaca cttttttgat aattctgctg cagaaaggtt tttaccagta
    19741 aaatctaaag aaaatggctt atcatattca aaaggatact caacaggtac accatctgga
    19801 gctaatgcaa ccataccata tggtgtattc ataaacccta ttacagaaac tgtatgcctt
    19861 gatgggtcta aatttgaatt tttgcttgta aacttaattg tatttattga tgagttgatt
    19921 aaactacacg gaactccctc atacctttca attttgggac gtagattatt tccataaact
    19981 tcatcaagta ttgcttcttc ttgatctgct atcatagtgt gattcccagg tccaactggc
    20041 gcagaaggct tagctggcgt aggtttctta acagttggcg ttggattctg tgtctttact
    20101 gattcaccaa cagctggata ggctttcttg tcaaatggta atgggtgagt aagactcttg
    20161 tatatgatgt ctgctcctgc tgatttgttg gcatcaatat ctacatgata caaacgtcca
    20221 tcctcaaaac gaataagatt ccactgatgg tcatttgttg tactatgtac ataataacat
    20281 tcaattccca tagcatcaca taaagctttc aagctcctac acattccaga acaagcacct
    20341 tttttagtaa ccaatgtata agcagtattg gctccctctg ctgcgttcca gttaacatat
    20401 gttgtgttgt ttttaatgtg attataagca accctaatct tagcatctgt ggtcatgttc
    20461 tttgtaattt tcttgttcat aaagtctgct gttgcttgtg ctaccgaatt aagttgagct
    20521 tgtgataact ttggaccata tatactgttg gttccagcta catagctttt cggtgtcttt
    20581 gcagtaacat ctttagctgt cataccataa actgttacac tcatacatga ggttaaaacc
    20641 atcgttgcaa tcaatagttt agtagctaat ttcctgattt ttttcatatt agcattctcc
    20701 tttataatat attttacttt caattcccta ccattaatta agttcatttt agatttaaaa
    20761 tatggattat caacatgtgt gcatagcaat tcacgtggtc atagaatatt tagatttctt
    20821 ggaaaatctg ttctatattt cctatatagt tcgtacacaa tccaactcac aaacctgcat
    20881 ttttgatatc atcacaataa aatgataata attaatatat tcaaaaatgt tggtctgtaa
    20941 caggtttgtc aattttctaa ttattggtaa tgacgagagt gaggagaatt gtcaataata
    21001 catttaaaat taaaattgta tacacttcat acattctccc ctaacaacat tatacagatt
    21061 ataatttgta tattcaatac attttttgga aggtataatt atatcaaaat atgtgattat
    21121 gtatagatac aatccactat ggagaaatat aaaagaaaag agtataacaa acaataaaaa
    21181 ataagatgat tcaaatttta ttttgaaaca tcttatttac tctacaaatt aaaattattt
    21241 ggtgaattat ttaattatta ttttttatta aagaaatttt aaatcttaat ataaaatacg
    21301 ctattataca taataatcca aatgcaaatg ctactggaat taacatctcc gattcaaaat
    21361 aaaagaaata ttctaagaaa atatctggat ttactatatt cccattcttg tctaaaaaat
    21421 cttttccatt atatcctaaa tgctgtataa aattacttat gttattataa tctgcttccc
    21481 tctcatatac catattagca ttcaatccag aagaacttaa attagtgttt ccatctttca
    21541 tatctattac atatagtcta agagctttga ctttactata cttatcttta tcaagtattt
    21601 tttcaagttc taacttatat tcaccaatat ttgtcatatc atgtctttct gccaattgct
    21661 ttatttctcc atcaagttct tttcctaaat tatacatatt atgttgtaaa ctattattgt
    21721 aataccacat aaatccagaa attaaaaatg caattatact tatgtaacct aatacttttt
    21781 taaaccccat tacaattgat ttatgcctat ccaatgataa agatagttct tttattatat
    21841 tacataattc atactgcatt tcatcaccat catcaaatct ttcaattgct tttttacatg
    21901 cttcttcttc atctaatcct tctaatttaa attcatttgc agattctata agatggcatc
    21961 tcatttcctg ttttaattct atagtacttt tattgtcaca ttttttatat agtttatcta
    22021 aatattcatc tattagtttc ataaattaat ccacctctcc caaaaattga ttcataacct
    22081 ttttaataaa tctccattct tgtttcttta tttctaaaaa atctttacct tcattagtta
    22141 aattataata ttttcttctt ccacctgaac tttgttcatt gctataataa gatttaatta
    22201 aatttttaga ttccattctt ttaagtgcaa gatacattgt tccttctttt aattcaaact
    22261 cacttctttc tcttacttgc tttgcaattt catatccata acaatctttc ttttctaata
    22321 tagaaacaat taatatatcg atataacctt ttaacacttc tttatttaac tgcattatgt
    22381 accaccgcct tttcacctac tactctataa tgtatagtac ttatatttac tataataata
    22441 catagtactc tataatacaa ggtttatatt ctacaataga cttaaaaata ataaatagct
    22501 gtatattagt atttatagat taaatactaa tatacagcta tttatcaaat tttaaaaata
    22561 aatttttgtt ttattaatgt tgtcgtaaca acatttactt ggttgaaata gtattaccta
    22621 taagtttaaa tatatataat aatagactta attctatttc atattattaa aatagagtaa
    22681 tagtttaaat tctactactc tatctataat ctatttccta attatttaat ctcaaattta
    22741 gctcctcatc atttaaatct ttcataagat ttctacaggt actaggttta ttgcaacaga
    22801 aaaactaggg cattaaacac ttatatatct gtttctttag actgtaatat taatattttt
    22861 taaatacgtt gacttttgct ttttctaact tctctccatg atagttctcc tctatcaaga
    22921 gcttttataa atatttctgc tgaagctaga tttgtagcta ttggaacatg ataaacatca
    22981 caaagtctta ttaacgcttg tatatctgtt tcatgagcct gagatgtcaa tgggtctctt
    23041 aaaaaaataa ctaaatctat ttcttgtgtt actattaaag aacctatttg ttggtctcca
    23101 cctaaaggac cagatgctaa tctatttata tttaattcag tttcatccat tattcttttt
    23161 cctgtagttc cagttgcata tagtccatac ttttttaaaa ttgattcata acctatacaa
    23221 aatccgacca tagtgttttt catttggtca tgtgctacta atgctatatt catatatcta
    23281 atctcctcaa tttatgttta gatgtattta aatttagaaa tttattttct ttcttctcat
    23341 acacacattt agtactaatc ctaagtttgc taaagatgtc aataacgaac ttcctccata
    23401 acttacaaaa ggtaatgtta ctccagttac tggtattaac gcaaccgtca tacctatatt
    23461 ttggattatc tggtaaccaa acattcccat aacaccaacc actatgaggg ttccataaaa
    23521 atcttttgca tctcttgcaa ttgctaaaag ccttaatagg aatatcataa ataagattat
    23581 caacactgcc attcctatta caccaagttc ttcacctaca acagcaaata taaaatcact
    23641 atcttgtaca ggtaaaaaat cttcttgatt ttgacttcca ttgtaaagtc ctttaccagt
    23701 tacgccccca gaaccaattg caatcagaga ttgcataact tgatagtttc cttttagagt
    23761 tacatcttct ggatttagaa aagcttctat tcttaccttt tgatggtctg ccatcattaa
    23821 atacatcagt ggcattgaca ctaatagtat gattattcct ctttttatca actttgagct
    23881 aagacctgct gtgaacagca ttgcaaatat catacaacaa aatacaattc ctgttcccaa
    23941 gtctggttgt gcaatcaata atccgataaa tggaagagaa taaactacaa caggcataac
    24001 ttcctttaac gtatttaatt tatccttttt ggattcaagt atctttgcat aacttagtac
    24061 aaatgttaat ttgactattt cagatgtctg caagtcaagt ggtcctaagt taatccacga
    24121 cctagctcca ccttttactg tcccaattcc aggtaataaa actatggcta acaaaataag
    24181 acttattata taaagcgctt tataatacct gcctagaaga ttatagtcaa aaaataatat
    24241 gactataatc attcctatac caagtacaaa agctaatcct tgtttaatca attgattata
    24301 tgaacctgtt gaattagcat gtgttgcact actaagtata acaagcccaa aaataaagat
    24361 agctaatact gtaactataa gtttccagtc taactgtttt atcaacttaa ttgtcgactt
    24421 ataattaata ttgatttttt tcaaagaatc accgtctttc tatctttaaa catataatta
    24481 aataaaaggg gcatatacca ataaggatat ccccctttta agcactaatt acatacatct
    24541 atcttttata ttctttagtg gtatattagc aactaatgca gatacaggtt tgtcatcatg
    24601 ctcacctctg catttagaca tttttatttc aagaccatca tcttctattt ctgcataatt
    24661 agctattact tttaaaatgt ctgtttttat catctctaaa agttgtggtg aacaatctac
    24721 tctatcatga actaaaacta gctttaacct ctctttagca acagatttac tagttttagc
    24781 ctcgttagaa aaaactctaa ataaatctaa cacagatgta tcctcctttg ctacttagcc
    24841 atgctaaaca tttttttaag tctaccaaag aatccatctt caacttcaag atcaagtaga
    24901 ggaacttctt catttagtat cctttttgcg atatttttgt atgcttgacc agcaagtgac
    24961 ttagaatcaa gtatagctgg ctctccttta tttgttgata taattatgct ttcatcatca
    25021 ggaacaagac ctaacaaatc tattgctaat atttctatta tatcttgttt atccatcatg
    25081 tctccacgct taaccatatc atttctaatt ctatttataa ctaatcttat ctcttttatt
    25141 tcatttgctt ctaatagacc tattattcta tcagcatctc ttactgctga tatttctgga
    25201 ttagtaacta caatagctct atctgcccca gctactgcat ttttaaagcc ttgctcaata
    25261 cctgctggac agtctattat tatatactcg aaagattctt ttagtttctc acacagatca
    25321 atcatctgct caactgagac agcattttta tctctagttt gtgctgctgg taataaatat
    25381 agattatcaa atcttttgtc ttttattaga gcttgcttaa gcctacaagt tccttcaact
    25441 acatctacta tgtcataaac tattcgattt tcaagaccca ttactacatc taagtttcta
    25501 agtccaatat ctgcatccac aactactgtt tttttatttt ctaggcttag cgcagttcct
    25561 aagtttgctg cggtagtagt ttttccaacc ccacctttac cagatgttat aactataact
    25621 tcgctcataa tactaccccc tatttttctt tctatttgtc taactttgac aaatagcttt
    25681 cgattacaat tcttccatta cttacaaagg ctatttctgg gctaatttct gattcatttt
    25741 cataattttc ttcatcatct ggagcttctg caatattatc tgctatttgt aatattttag
    25801 gattaagatt tccagcaaca acatatgcaa attcattgcc ttctgcacct gcatgtacaa
    25861 aaccttctat tttacccatt acaaaaacat tacctcctgc tacaacctta gcaccagcat
    25921 ttatatctcc cataacaact gtatttgatt ttgaaaggac ttttgaacca ggtttcatat
    25981 cattcaatat aactacatca ccttcaaatt caatattttc accagatctt agggtattga
    26041 catattttgt tggaaaatta ctattttctt caattttttg atcttcaaca aattccacat
    26101 caaatctaga agtaattcct tctttaagtt ctagaatctc catgtcagtt aaacaatcac
    26161 tattaatttt actaatttta gccccattaa aaaagccaac atatgcttcc aatttgttta
    26221 ttattttttc ctgtatttcc tcaaatggag cttctctttt tatatttact attatccctc
    26281 ttttattacc cttgaactct actagttctt gactacaaat ttctcttaaa gacataacaa
    26341 cctccagagt gatatatcat ttatattctt caaatacgct ttaaaccctt ttattttttc
    26401 tatataaagc taaagtttat acatagatta agctatctat taatttgtga ctcgaaattt
    26461 atattttcgt cagaagaatt gttaacatca ttcgtcttgt tgtcattttt actattagtt
    26521 ggctctaatc ccatataagt tccaataact tctctagttg gtaaaagtga gaatacactt
    26581 gaatcacctt gaggaattac acatacaaca gctatttctg ggtcatctgc tggagcaaaa
    26641 gcaacagtcc atgtaaaaga accataatct tgtttaaatc tatctatttg gtcatctgtt
    26701 atttttggat ttaattcttt tatagctttt cttagatatg aagaattcac cttatcagtg
    26761 tcctctaatc taacttttac tccatcttct aattcttcct ctaatttctt tctttcttcg
    26821 tctttcaaat ctttattttc taatttcttt tttatttcct tttccttagc taatgaaagc
    26881 tctttttctt tttctgcttt catcttatca gctagtttaa tcgcatcatt taggttgaca
    26941 ttataagatg acatatgact ttttaaatat tcatactcgt tatctgtagg tattttccca
    27001 cttttctcag ctgtaccagt ttttgctgct acatctattg gaaaatcagc aaaagcactc
    27061 ttcgctgtac cttgtcttgc aaccaatttc ataccttttg ttaattcttt taagttatct
    27121 ggatttttaa aaggtatttt ttcgactttc ttctggtcat taattttaac tgatgaataa
    27181 tcacttgaaa cagctctatc aactacagat aattctacta aattacctcc atttactata
    27241 gcagctacat atcttgatat ttgagctggt gtataagcat tttcaccttg acctatagct
    27301 aagttaaatg tatctgcagt actccatttt gcaaagttaa aataactgaa tactgctaaa
    27361 tctgctactt cttctattct atcttcttta acattcattt tctttagcct attcattgtt
    27421 tcgactctac caggagtttt cttttctgct gcccagctta caatctcatt gattcttttc
    27481 tcatattctt taggattttt ttctctagtt atatcaacaa aatcatttgc catttctctt
    27541 tctaaatttg actttagaag agcttgtgtt gatttcaatt tatcttctgt gctaggaact
    27601 ttacctgctc tttcttctac ttcatcttga agccctgtat tttggtcaag cccaaataat
    27661 tttgcatact caagtatttt atttgcattc atatctatac caatgctttt tccatctggc
    27721 catgttttac ctgtaccaat tgttgccata tatatattac aagactcttg tatcgcttta
    27781 tataagtttg tcataccatg gtttcctcta ccatggttcc atacatagtc accaaaagat
    27841 tttttaccta acattataac cccagggtca tttatagtaa agtttggatc caaaccattt
    27901 tctaatgctg ccattgaagt taccatctta aatgaagaac ctggctgaaa ttccccttgt
    27961 gtaaccaagt ttaaaagtgg acttcctgct agtaagtcat ttggattttt tggttgaagt
    28021 gctttataat cctctgtaga aatacctttt gcaaatttat ttgggtcata attaggataa
    28081 cttgatgaag ctaatacttc tccattttta acatcaatag ctataagagc tgctgactgt
    28141 gcttttcctg catatgcact aattggtttg tccccaaatt tacttttaaa agtacctcct
    28201 ttagaagcaa cttcgattat ctgttttaat gcattatctg aaacttcttg taaatcttta
    28261 tctagtgtaa gatacacagt atctcctgat ttaggttttt tagattcaat ttctttactg
    28321 attctaccca atgcatcaac tttaaccatt ttataaccat cagttcctct taatctactt
    28381 tcattactct tttctactcc tgcaagacct accatatctc cagtttcata acctttttga
    28441 aggtatgatt ctatttgagt ggatggcatt tttcctacat atcctaacat atgagaagct
    28501 aatgacccat taggataata tcttactggt tctactgcta ttgaaacacc aacaaggtcc
    28561 atagcacttt cttcaatttg agcaatcgta gtttctccta catccttagc aatagttact
    28621 ggattatatt gagaatatcc ttttgatttt atcaaatccc ttacgaccat tatttttctt
    28681 gcatcttcat cactcaaact tttatcaatt tctaatgcat cactatttct tactttttca
    28741 aacgcttctt tggcactcag tttagtttct tttatcttat aactagccaa ccaatctctc
    28801 ttatctcttt ctgctgtgaa catccattta ctaactaaaa taggtggata atatccattt
    28861 tcatttagtt ttgcctgtaa tcttgtcgca tccaatcttt tatcttcttg agataatatt
    28921 ccagctgata taagtttgtc tactaagtaa taaaaacttt ccttagcatt ataatcattt
    28981 ggaataccat tttctgattt atattcccta atatctctgt catatgtata atagtacttt
    29041 ccattttcta catatatagg aaactcatct acatattctt caccatttct ctccagcaaa
    29101 tttataagtt ttaatgatat ttcatttgct cttgaatgct tatttgcatc tttcttatta
    29161 atatcgttac ctgaaacttg aacagtaaat aagtttttat ttcctgccaa cagtcttcca
    29221 tatctatctt ttatttctcc tcttggagct tctattgcca actttttata tgttttattc
    29281 tcagctaact cattgtaata ttcatattta aatgtagtca tatagattat ttttactaaa
    29341 atagctaaaa atatcacgct catgactatc ttaattattt ttagtctatc aacttgttta
    29401 ttttcttcca tgaaatcacc aactaatctt ctttaagttt taaaactgct cttttagata
    29461 agcgatatat tactaaacca attaaactat tcactattgg tgctactagt agacctttta
    29521 caaaaagtgt tgatatgttg tagctttgat aaattatact agatgtacct atattgacaa
    29581 ttgaatcaat taaactgcct ataaaaatta aaacgaaagt aataatataa ctacctttat
    29641 atagctgatc tctcatgtat ccaattgcat atgcaataat aaacaaaatt aatgcattta
    29701 caccaaaaat tccaccaacg gttatgtctt taataagccc tattattgct gctataatcc
    29761 caccttctaa ttcatctaaa taaagagaga ttatggttac atatataaga actaaattaa
    29821 agcttatgcc aaaaatatct atataatttg ttatactatt ttctatagtc ataagcacaa
    29881 tccccaacag acaaagtaaa acttttttca ttgtttttat cccctttatc ctatatttct
    29941 tggttctatg actacaacgt catttatatt cttaaaatca acataaggtt ttacaactac
    30001 atattttaat gacttatctt tatcatcaac caccttttca acttcgccaa ttggtatacc
    30061 atctgggaag aatccaagac ctgatgttgt tattatatct ccttgaatta cattatatga
    30121 tgagtcaaac atatatcctt gcaaatatcc tttactatta taactatctt tattatctaa
    30181 agtagtattt tgagtaattg tacctttagc attggcatcc tttgctagtt taaagcttac
    30241 agatgcctta gaatccaata gtgaaattgc cttactataa ttgttggaaa cttcataaac
    30301 tatgccaact aacccactac cattcataac tatactttct ttttttacac ctgaattttt
    30361 tcctgcacct atgacaaaac tctcatacca gtttccatca tttttaccaa caacacttgc
    30421 tgaaatactt gtcgccttat actcttcttg tacaaagttc agtgtctttt ttaactcttc
    30481 tagcgattct gttttatcta gtttggcatt taaagctatc atatcttttt taagtttttc
    30541 attttcttct tccaattttt ttaccttttt agaattatct ttaaaatttg ctacatcttt
    30601 aaaaccattt ttgataaaag aaaatccatc atttattccc ttacctacac ttgtaatagc
    30661 atctgaagct actcctaaat ttactggact accactagaa aatttaccaa ttgatatacc
    30721 cacaattcca attaaagtga tagcaactac gcccgttgct atcactttaa cattaatcct
    30781 ttttttctca tttttgttct tgtcaaatct caaggccatc accaacttct aattcttagt
    30841 attcatcatt aatacttttt caaagatttc ttggtcttca acagatttac ctgttccaag
    30901 tgcaacacaa tcaagaggtg tttctgctat ttgtacagac ataccagttt cctgtgtaat
    30961 aagcttatca agacctctaa gcaaagcgcc tcctcctgtt agcattattc cgttttccat
    31021 tatatctgag gctaattctg gtggagttct ttctagtgta gattttattg catctactat
    31081 tgaacttaca ggttctttta atgcttctct aacttctgta gagcatattt ctattgtttt
    31141 aggtaatcct gagattaaat ctctacctct tatttgcata tttatttctt catcatcttt
    31201 aaatgtagaa cctatgttaa tttttacatt ttcagcagtt ctttcaccta tcataagatt
    31261 atattctttt tttacataag ccactataga ctcgtcaaat tcgtctccac ctattcttat
    31321 agatttagca gtaacgattc ctcctaagga tattacagct atttctgaag tacctccacc
    31381 aatatcaact accatattac cttctggttc ttcaactttt aatccagccc ctatagccgc
    31441 tgccattggc tcttctataa ggaatgcatc tttagctcca gcttgtcttg cagcctcttc
    31501 tatagctctc ttttctactt ctgtaactcc aaaaggaaca catatagcta ttcttggact
    31561 tacaacacct tttttatcag ccgctttttg tataaaatag ctaatcattg attgagttat
    31621 gtcaaaatca gctattactc catctttcat aggtcttata gctactatat ttcctggtgt
    31681 tctacctatc atttttttgg cttcttcacc tactgctaat acctctttac tatcatctct
    31741 tattgcgaca actgatggtt ctctaacaac aataccttgt ccttttatgt aaactaatgt
    31801 atttgcagtc cctaaatcta ttcccatatc ctttgtcatc ttattaaaac taaaaaaacc
    31861 ttttttttct ttcttttttt ccttagccat gattttactc cttcctacta ttaagaatca
    31921 tgtttaaaac attctcctat tattttatca catataaagt caaattatca tattttcttt
    31981 aaagctaaag tataatccat ctccaataat tatatggtct attatttcta ttccaattat
    32041 ttctccacat ttaataagtc ttgatgtgat atttttatct tctcttgatg gttctacact
    32101 tcctgagggg tgattatgca tcactattat cttacttgca cttcttctta tagcttcttt
    32161 aaaaacctct cttggatgga ctaatgacga gcttaaagtc cctatagata catctacatc
    32221 agatattatc tcattttttg tgtttaaaag aactgcttta aaaacctctt tttttaaata
    32281 ccttaggcta tccatataat acctctgtat atcccaagga ttccttattt tatactttat
    32341 aggtttaaaa ctagctactc tagagcctag ttctaaagct gctttaattt gggttgattt
    32401 tgataaccca attccatcta tattacacag ctcctctatt gtcatatctt ctaaatgtct
    32461 aataccctga atatctctat ttattatata gttggctaat tcaatagcat ttttattctt
    32521 gttgccagtt cttaaaagta tagccaacaa ctcagcattt gataagcttt ttacgccttt
    32581 ggcaagcatc ttttctctgg gtctttcctc tggtgccatt tcttttacct taatggttga
    32641 attaaatgac tttttcagat aaatcacact ccataaaaaa gatttatact aaaatatttt
    32701 tttaataggt cgcctaacct tgaaattgga agaccaacta tattaaaata atctcctcta
    32761 atttcttcaa ctaacaaagc tccataaccc tgtataccat aggctcctgc tttgtcaaat
    32821 gactcattag ttttcagata atcctcaata tcctgctctg ataacgtttt aaatttcaca
    32881 ttgctgatta catagtcaat taccttttta tcttcgcaca agtttattaa gctaatacct
    32941 gttatgactt gatgctctct tccagatagt ctttttaaca tatctcttgc acaagactca
    33001 tcttttggtt ttcctaaaat agcattgtct aaaacaacaa ctgtatcagc tccaataaca
    33061 acatcttcat tatgttcaga tgctacagac atactttttt caaaagctaa tctcataact
    33121 aaatgttttg gtgtttcacc ttccaatata atctcatcaa tttcattttt aattatatca
    33181 aatcttacat tcgtattctc tagaatttct ttccttcttg gagaggctga tgccaagatt
    33241 atattcattt attcacctca tttgtacgta atttgtacct atttagtcta tcactatatt
    33301 gtaaaaaatt caacagaaat gtcgaacctt ttttgttaca ctattgttat atttattacc
    33361 attagaataa atatacataa aatagaggta tggaaattat tccatacctc tgttttatgt
    33421 actcattaga gatatggaaa ttattccata tccttatttt gtccatatta caaaatttta
    33481 atagctttct ttggacattt ttccatacat aaatggcatc ctacacattt atctgcatct
    33541 accttatgtt tttcttttaa ttctccctct attgcatcaa acttacattg ttttttgcac
    33601 actgtacatc caacacaaag ttcttggtca atagtaactt tctttttctt agttatgtca
    33661 cctgatatta cttttgtagg gcattttgct acacattgca tacaccctac acacttatta
    33721 gggtctatct tagctatttt gtcttcaaat attattgcat caaatttaca agcttttaca
    33781 cacattccac agccaataca tccagcagta catttttcct taacagcttt accaaactct
    33841 ttgctattac actctacaac tacttcttga ctttctggtt ttgtgattat tataccctta
    33901 ggacaaactt ctttacattt tccacaattt acacactttt cttcatctat tactgctatt
    33961 ccatcaacta tagaaatagc atcaaattta caagcatcct tacatgttcc aagacctaaa
    34021 catccaaatt tacacatctt agcacctgag tttaaaacat ttgctgctct acaatctgat
    34081 attccttcat actcatattt atcttttgca cttgaacaag ttcctttaca aataactttt
    34141 gctacttgtt tctcacctgc ttcagcactt attcccatta tttctccaac ttttgcacca
    34201 acatctgctc cacctacagg gcaaccatta actggtgcat tgccttcaac tatcgcattt
    34261 gcaagacctc cacaacctgg aaatccacaa cctccacagt tggctcctgg aagtactcct
    34321 agtatagctt ccactctctc gtctacttct acagcaaact ttttagatgc gaaatctagt
    34381 actatcccga agattaagcc cataattcct aaaactaata cagctgtaag tatcaccatt
    34441 ttcacccctc ttttttatag ctttataagt cctgtaaatc ctaagaaagc tattgacatt
    34501 aaacctgctg atataagcgt tatagggaaa cctttaaatg cttctggtat atcagctaac
    34561 tctagtcttt ctcttatacc tgcgaatatt actattgcta aagtaaatcc agctccggct
    34621 ccaaatccat ttattattgt ttctactaaa ttataacttt tttgaacatt aactagagct
    34681 ataccaagaa ctgcacagtt tgtagttata agaggtaaat aaactcctaa tgcttgatat
    34741 aaagatggac tcatcttttg aattaccatt tcaacgaact gaactataga agctataacc
    34801 aatataaaag ctattgtttg taagaatcca gttccagttt ttactaataa aatctgtata
    34861 aaataagtta taattgaagc tagagttaaa acgaacgtaa ccgcaactcc catccctact
    34921 gctgtatcaa ccttcttaga aactcccaag aaaggacata tacccaagaa ttgagatgtt
    34981 ataacgttat taacaagaac gatacttaaa aataaaagta ttaaattcat ttattacacc
    35041 tccactatgc ttttttactt cttaatttat taaatcctgc aaataagaat cctaatgtta
    35101 agaatgcacc tggtggtaat ataaatatta atactggttg gaaagatgct ccaaataatg
    35161 tcattccaaa taaactacca tttccaagta attctcttac tgcacctatt attgtaagtg
    35221 caacagtaaa tccaagacct tgacctacac catcaactat tgaaggcata gctcctgttt
    35281 tgaaagcaaa actttctgca cgagctaata ttaaacagtt aacaactatt aatggtatat
    35341 atataccaag agccttatca agagcaggaa catatgcttt taaaagcata cctactattg
    35401 taacaaatgt agctataact actataaata taggtattct tatcttatct ggtgtaattt
    35461 ttcttattaa agatataact aaatttgcac atattaaaac tgctgctgtt gaaagaccca
    35521 taccaattcc atttattgct gaagttgtaa cggctagtgt tggacacata cctataactt
    35581 gaacaaatgt tgggttctca tctattagcc catttttaaa tactttagct aaattcataa
    35641 ttaaccctcc ttatttatta agagcactat tgaaaacatc tatagcagca tttactcctg
    35701 tagtaactgc tgtagatgta atagttgcac ctgatattgc taatatttga ttatctgctg
    35761 ttgtacttcc ttttgctact tcaagaggct cagcagtttt tcctttgtat tgacccttaa
    35821 atgcatcatc cttagctttt gctccaagac ctgctgtttc tgacatatta ccaatgtcaa
    35881 cacctgtaac ttgaccttca gatgatatac ctaccataac ttcaatttct ccaccatatc
    35941 cacttggttt agcttttaaa gtatatccaa ccacttcaga accatctaaa ccttcataca
    36001 cttcagctat aagaccatct ccaaggtctt tatatgtact tttatccatt tctttaaatt
    36061 cttttgcttc tggaaggact gcttttctaa gttcattatt ttcttgtata tttctttgtt
    36121 ctattactgg agctgtaacc tgattagtag ctccaagagc taatgcagct attgcactta
    36181 tagcaagtaa agtaccacct aatctcacca tactgttcat tatttagcca cctccccaaa
    36241 tactctattt ctaacaaact tatctataag tggagcaaga cagttcacta atagtattga
    36301 gtaagataca ccttctggat atccaccata cattcttata acagttgtta taagaccagc
    36361 taatacagcg tatattattt gacctttttt agtcataggc gaagttgtat aatccgtaag
    36421 catgaagaat ccacctaaca taagtcctcc agcaaacagt tgatatattg cgaaagttat
    36481 attaaatcca cctaaaagga atgttaatat aaatacagtt gctatgtaaa atactggtat
    36541 aacataattt attatgcctt tatacattag gtatactcca cctaataata ataatattgc
    36601 tgatgtctca cctatacatc caccaatatt ccctatcatc atgtcagcaa gactaattcc
    36661 attgctagaa aggtctgcca gtcctgctgc tccatttttc aaaaagctta atggagttgc
    36721 tgtagtaact gcatctaaat ttttacctat ccagttaaca cctggttgag tccaagcagt
    36781 cattgctact gggaatgatg ctaataaaaa tgctcttgca gcaagtgctg gattgacaaa
    36841 gttacatcca attccaccaa ataccatttt aactactaat attgcaaata ttcctcctac
    36901 tagacacatc caccaaggaa gtgatgctgg tacgttaaat gctaataata atcctgttac
    36961 aacagctgaa aaatctccta tagtactttt ttgcttcgta aatttttgat ataaaaattc
    37021 agtacctact gtaccaagta tacaaaaaaa catagcactc aaagcattaa gtctaaagaa
    37081 gtatactcct gctactgctg ctggaaggag tgctataata acttgtttca ttatatatga
    37141 agtatcttca ttacttctca catgaggaga agatgatact atcaacttat tttccatcgt
    37201 ttttccccct taactttctt tatttctgag cagcttgctt tcttctcttt gctcctattt
    37261 ctctctttgc tgatcttata gattgaagta atggtcttct tgctggacaa ataaatgaac
    37321 aagacccaca ttctatacaa tccattgctc tatgatattc agctgtgtca tagtcatctt
    37381 ttaaagaata tgcacttatg aaaagtggtt gtaagaatga tggacataca tctgtacatc
    37441 ttccacatct taaacagttt tgaatatctg gagtacgtga ttcttcctca tccaagcata
    37501 aaattcctga agaacctttg ttagtcgcta tttcagtagt atattgagct attcccatca
    37561 taggtccacc cattataacc ttaccaatct tcttatcttc tttaaatcca ccacattgtt
    37621 ctattatttc tgaaactaat gttccaactt ttgttattag gttctttggc tctttgatgc
    37681 aactacctgt tattgtagtg attctttcaa ccaaaggcat acctgtttta atagacttag
    37741 ctatttgtgc tgctgtggca acattgtcaa caactgcacc tgctgctatt ggtaatccac
    37801 cagatggaac ttctttgcct gtacacgcat aaataagttg tttctctgct ccttgtggat
    37861 atttcacttg aaggccaaca acttcgattt cactatagtc tttagctaca ttttgaattg
    37921 cttctatagc atctggttta ttcgtttcaa ttcctataaa tccttttttg acatctaata
    37981 ccttcattaa tgctcttaaa ccaaacacaa catcttctgg attttcaacc attaatctgt
    38041 ggtctgcagt taagtatggt tcacattctg caccatttaa tataactact tctgccttgc
    38101 tatctggtgg aggagatact tttacatggt tagggaatgt agctccaccc atacctacta
    38161 ttcctgcctc ttttatgata cctactatat cttctttgct caaatcctct aactgacctt
    38221 ttggttgaac actctcatgt aattcttcct taaagtcatt ttcgataact acacattgag
    38281 ctgaacctcc tggtacttca tgttgttcaa tagcaataac tttacctgat actgaagaat
    38341 gaacgttaca tgaaacaaaa ccttgttgtt cacctatttt ttgcccaaac tttacttcat
    38401 caccaacttc aacaataggt ttagctggtg cccctatatg ttgctgaaga ggaatgtaaa
    38461 ctatctttgg agcttgagct ttttcaagtg cctttgtgtt agagtactct tttctatatg
    38521 gaggatgtat ccctccctta aaagttaaga gtttcattgt tttaccccct tatctataaa
    38581 aaaacatttt ttctgccttc tataagtata ctatatatat taaaaaatct taacaattgt
    38641 taaaaaaata tccgtatttt tataatttat tttataaatc aatacctcta tattaagtca
    38701 aactgaggaa aaggattgct taattaaaca atccttttca tatagctttc taaagtccaa
    38761 attttctgta ttttatttta tagaatttat aaacatagag tactcctgca tggatgataa
    38821 aagcagactt ttacttacac tatatttttc ttcatttgct gcttttgaag ctgtatcaat
    38881 tttttcattc acattcttta catactctat taaattatct ttaaataaac gcatttcaga
    38941 atagtcaatc ttttcagctt cttttgatat attttgtgat attgcctttc tatttgtcaa
    39001 aatagtatta tatttttcca tatctacatt attttcagca ttcgaccaaa aacttgattc
    39061 ttctttaaag tttgttatta atttatttaa ttctttagat atactctcta tataagcgta
    39121 gttacttgta tactctaaag acaacatagg tgcatctaag tgtgataaaa atgcatctgg
    39181 aaacaccttt cttacactgc ttatctgctt tctaacatca ttttcatcaa aagaggaata
    39241 ggtctgtatt ttttttactc catccttctc cataactgaa aaaggtatgt cattattaac
    39301 taatgatgtc tgtatttttt tcaaatcatc attattatct attgcagcaa cttgaatagt
    39361 gtacatatcc cagccttcta tagtagctaa tttgacttct tctggctctt tagtgttatt
    39421 taccgctttt tctggattag tttcttgttt atcatctgaa ttggtttgtg atttagattt
    39481 tttccctttt tttattgaat ttaattcatc agatatatct ttataagtta tatctgatga
    39541 attatttaga aatggtattt tagccgatac atactccaat atcttgctat cttttatttt
    39601 tgtataagaa taatagccaa ttagacacaa gcaagcaatc gttataacat atttacgtaa
    39661 atcaatctta tgtctattat tattaaatcc tctgtatatc gtttttcttc tattcattag
    39721 tttgtccctc ccaaaattat attattaata aagtatattt ttaagagtca caatttatga
    39781 atataaataa ttatttcttc taatttttta ataagaaatt gtacttacta aacaaatctt
    39841 cttgcaccaa gtaatacttt agaccaatat gaactagtta aattatccac tttaacctta
    39901 ttaggattag tctgagtacc actacaatgg ataaatttac tatttcctac atatatacct
    39961 acatgattag ttgttccatc accatcagtg tcaaaataaa ctaaatctcc tggtgcataa
    40021 ttccccatag aaattgcact tccaaaatta gcctgttctc tagaaactct aggaatagaa
    40081 acgcctacag aatgtttaaa aacatactgt gtaaatccag aacagtcaaa tttatcaggt
    40141 cctgaagctc cccattgata tggtattcca ataagagtat atgcaaaatc tacgattgtt
    40201 gaaccttttg ctgtagattt atctgaattt ccagtaaatg atggctttga gttactatta
    40261 gattgagaat tttggttaga tgatgaattt ggtttttctt ttgtaccttc attagtctca
    40321 gaaatataac tggcactcac ccatccaatt gttccactcg ataactttac cttataccaa
    40381 ccattatttt tggattttaa ttcaacaaca tctccaccat ttaattttcc tattatagaa
    40441 tagcttgtcc caggaccact tcttacattt aaactcacag cagatgttac cttaccattc
    40501 ttgccttcaa ttgattcttc gctagatggt tttttatcag aattattagt actatttgaa
    40561 ttactagaat tattcgttcc aacatcctct gatgttttag agatatattg gctactagcc
    40621 catccagtaa ctccatttga tagttttatt ttataccatc cattgctttg ttctagtaac
    40681 tttactacat ctccattatt tgctttacca actaatgaat agttagtacc tgcaccactt
    40741 cttacattca gtctagaact cacatttact ttaccatttc cactaatagt tgtcccactt
    40801 gaagttggat tattttgatt tgatgtacct tcagatgaac cacttgttga tatgtatttt
    40861 gcgcttcccc atccaacaac agaactcgat acccttactt tataccatcc attagacttt
    40921 tctaaaatct ctacagtttt tcccttgtat aattttgcta ccttatcaca atcagaactt
    40981 ggtccacttc ttatattcaa ggcacttgct gttactgttc ccttttcaag agcacttgca
    41041 tttatggagc taacagatac tgctactgcc ccaattccta aagctgctat agctttcttc
    41101 actactatca ctccccccaa caaattttta tagtcataaa ttcataacat tattatccat
    41161 ttttagtccc tacaatttaa tgataactta ttctcttatt aattgtaaag aaattataaa
    41221 catatcctta aaattaatga tttttaggaa gtatttcaat ccaataatta tctgggtccg
    41281 ctataaaata aattcccata ctttcatttt caaaacatat gcaatctaaa tctttatgaa
    41341 gtttttttgc ctcatcaaac tcctcaactt ctaaagccag atgaaattca ttgtctccta
    41401 aattatatgg tctgtcccaa tctctaagcc aagttaattc taatgtatga ctagtaatac
    41461 catctcctaa ataaactaat ataaaacttc catcttctgc ttccttacgc tttacctctt
    41521 taagccctaa agctttttta taaaaatcta aacttttttc taagttagta acattgaaat
    41581 tgttatggca aaaattaaac ttcattttga aatcactctc caatttttat tttttttata
    41641 ttatatcatt attataaatt tatttaaatc atattaagtt aaaaatttga atgatgatga
    41701 ccaattacca aatggtggta aattatttaa aagaatatgt caaaaaaaga ataagctctt
    41761 tcaaatgcac aaaaaattta tacccaagtt atacttaatc atgcaaattg gcagaactta
    41821 ttctttaaaa ctctaacttt taagtttttc tttgataata taatttatta ctttttttct
    41881 aatatcttca gcattataat catcttctac ttctatatcc aaaagttttg ctattgacct
    41941 catatcttct ctagtctttg acctatagct tgatattaat tcatctttta ttttctctgg
    42001 ttctaaaaga tattcttcat ctcctctttt gaaaacaacg tatttttgag catatgaact
    42061 tctattatag tatatatatc ttgatatttt tcttaaaatt tgattatatg aaagtcgctt
    42121 gttaatatca agttttaagt actctgaaaa agcaaatagc tctttttttg atttaaataa
    42181 tgcttctttc tttatagtcc ttgatattga agttacactt tcttgatatg cacacttttt
    42241 taacattttt aaatatactc tattaagacc aatactttga ttgaattttt ttatattatt
    42301 attaataatc ttatccaagt taccttcaaa gtattttttt gctccttttt gtgatacata
    42361 actctcaagt tctttgtggg ccatatcaag tatcattttt tttaaatcct ctttatatcc
    42421 cctagattcc atgatactcc ctcccttagc tatttatagt atattcaaaa agctcaagaa
    42481 atgtgagtca ttacaaaatt tttttacttt ttatgtatat attaatttaa aaggggagat
    42541 aatagtaatg taaaatcaaa catactcaat ctcccccatt tttagacctc tattactccc
    42601 ccaatagagg ccttttttta gtttatcaat ttctattcaa ttccctttag ttcaaatcct
    42661 aattctgcta tcacttcttt taatttttca atagatatat catctttcat atctacagat
    42721 gcacacttat tttctaaatc cacatctaaa acttcaatac catctatatc ttctgttaat
    42781 gcagttttaa gatggtttac acaatgacca caactcatac cttctactaa tagtttcttc
    42841 ttcatttaat tacctcctta taccccataa gggtattcta tcatatagtt tatacttttt
    42901 aaatagaaat taatcattta atttgattca ttcgatttta tatctaaatt agatattccc
    42961 tcatatacat caaaatcatt tggagtaatt ttacctttta atctactatt acttacaata
    43021 tagttgtttc tcacagctaa acttataaat gttgcattat cttttatata tgtctgtaca
    43081 tctttgtatg ctttttttgt ttcaatttca gacgtagaac ttgtaagtgc agacatatat
    43141 ccatttagct tatcatcagt atatccacta cttgctataa tactacttgc atctggtata
    43201 attgatagtt tccatccaac taatgctaaa tcataatttt tagaattaag agcattttcc
    43261 atatccttat ctgacagctg atttactgta ctatttattc ctatagcttt taagttttca
    43321 tttattatgt ttgcagtttt tattctctca ctattatctc tgtttactat tatctttaag
    43381 ttcatttcag aaagactttt ctttacttcc tctttttctc tatttttttt cttttcagct
    43441 tcttttttga ttctatcttc ttctgctttt ttagcttttg cttcttcttc tggcgtaagc
    43501 ttctttgagt tctttttagc tgattttttc tgatttttta ctttattgtc attttgattt
    43561 actgagttta tatttgcata ttctacctta gcaagacact ctatcgcctt atctttatta
    43621 aactctaaat ccttcatttc actattatag tattttgatt ttgaattaag tggaaaatta
    43681 actggagtag catcatccat ataaccttca ctaataattc tatctttatc tatagagtga
    43741 gcaattgctt ttctaaaatt aacatcttta aaaaatggat tattataatt aaataataca
    43801 cactcataat ctcttccttg atattttgtt atattaaact ctttttcttg aaacttgctt
    43861 aaatcattta atgtaacatt ggttatatca ctatcaagag ccataaccat agatgtcctt
    43921 gcctcttcat taggcaccat acctactttt atgttcttca tggtctttgg aacttcttca
    43981 tagtaatctt tgtttacaga taaaaccatt ccttctcttt ctgtatatga ttctatttta
    44041 tatttcccat ttcctatcat attattattc ttgtcattta catcctttga atctaaatga
    44101 ttttgagata cgattggaaa aattaaacta tcaattgaaa aagcatatct agctttaaat
    44161 tttattgtaa aatctttatc tgatttaata ttcacagaag ctatattttt agtaaattca
    44221 ttatatggac tatctgcact tttttgaatt aaaccaatag tatagtttac atcatttgaa
    44281 gtaacatttt ttccatcatg ccactttgca tcttttagct ttatatctat actcattcca
    44341 tcctgagcta tattatactc cttaactaat tgaggaatta cattgtagtt ctcatctata
    44401 gtaaataaac tatcataaac taaattcatt atataaccaa cagacttatc agtattagtt
    44461 ataggattta tagtttttgg agttaccatt gttaagttta tatacttagc atctatagaa
    44521 ctttgacttt gtaaacttga atcactacta ctttgttttg ttttagtatt actacaacca
    44581 gctaccataa gagttatagc taaaacgact gttaagactt ttattctctt cattagtttt
    44641 ttcctttctt gccatatatc ttattataat gttcgtaatt attttttact ttttctacat
    44701 attttgaagt ttgcttaaaa ggtatatcat gtagattttc gccatcttta ctatattcat
    44761 tattttctaa ccattttttc acattacctg aaccaccatt atatgcagct atcactaaat
    44821 ctaatttacc aaattctttg tataacttac ttaaatacca acagccaagt cttatattag
    44881 tctctgggtc gaaaatatcc acatttttta aatttagttc ctctgctccc caatctctag
    44941 ttatatctaa tatttgcatt aatccttttg cttccttttt tgaaacagca gaactattaa
    45001 acttactttc ggctttaata acactgtaaa ctatattttc atctaaatta aattcttttg
    45061 aatacttttc tacatactct gaatattttt taggatataa aaatttatgt attactttgc
    45121 tttccattaa tagtgcccca aataagatta taaaaataga tagaatcaat actttcttac
    45181 tattcacgcc aattctcctt catgtactca ataaatttat gtgctttact ttctaattca
    45241 gttattgttc ccgaattatc tattatataa tcaccatatt tggatttttc ttcctgactc
    45301 atctgtgaat ttatcctact aagtgcttcc tgcttactac aattatctct cttttgtatt
    45361 ctactaattt gtacttcttg tttgcaagta actactaata gcttgtcaac catatctaaa
    45421 aaaccacttt ctaccaatat tgctgcatct aatactacta tattttcgcc tttttttctc
    45481 aattcttcta tttcacttat tattttttct cttattcgcg gatgagtcaa gctatttaat
    45541 tccttaagtt tttcttcatc gctaaaaact attttcccaa gagcttttct atttagagtt
    45601 ccatcatcat tttttatact ttgcccaaaa tggacaaata ctttttccaa taacaactta
    45661 tcctcaaata tcttcctgga tataatatca gcatccacta tagaaatatt aagatttcta
    45721 aatatatttg ataggctgct ttttccacag cctattcctc ctgtaagtcc taatatcaac
    45781 ataaaatcac ctactttgtc tcataccaag aactacctac atttaaatct acatctaagt
    45841 gaacatccat atttacagca ttttccattt cttcttttac tattttctta actatatcta
    45901 tttcatcatc aattgcttca actatcaact cgtcgtgaac ttgtagtata agttttgatt
    45961 tcaaattgtt ctcttccaat tttttatgaa catttatcat agcaattttt attatatctg
    46021 ctgcacttcc ttgtattggt gcattcattg caaatctctt tcctctattt ctttccataa
    46081 aattgcttga ctttatttct ggtatatatc ttcttctgtt taaaattgtt acagcatatc
    46141 catcctcact agccttttct actgttgtgt ccataaactc ttttatttta tggtatttag
    46201 caaaataact ttcgatatat tctttcgcct ttggaacagg tatatttaaa tcatcagcta
    46261 gaccaaagtc acttttacca tatataatac caaagtttac agctttagct gcacctctca
    46321 attcactagt aacatcttcc ataggaacat taaaaacttg tgaagctgtt ttggtatgaa
    46381 tatcttcatg atgtttaaat gcttctatca tattttcatc ctgactcata tgtgcaagaa
    46441 ctcttagctc tacttgtgag taatcggcat caaccaactt acagttatca tctgcaataa
    46501 acacttttct tatttttcta cccatttcca tttttatagg tatattttga agatttggtt
    46561 ctgtagatga aattcttcct gtagtcgtta tagtttgatt aaatgatgaa tgtattctat
    46621 tactttctgg atttattata tttaaaagtc cttcaacgta agtagaattt aactttacta
    46681 tttgtctata ctcagttatc ttatctatta ttggatgctt atctcttaat ttttctaaaa
    46741 catctgcatt agttgaatat ccagttttag tttttttaat tattggaagc tctaattttt
    46801 caaataatat aacacccaat tgttttggtg aatttatatt gaatggctct cctgataatt
    46861 cataaatttc cttttctagt gtagctatta tttctttaaa ctctacactc aattcatcca
    46921 atatactttt gtctactttt ataccttcat actccatatg acctaagact tctactaaag
    46981 gcatctctac atgataaaaa agaccatcca tactcatatc cattagactt ctttccatca
    47041 taggaattgt ttctttaact gtgcatatta tttgacccat atactctgct aaatcctcaa
    47101 attctaaatc ctcatagttt ttagatttaa caccctttcc taataattct tcttttgatt
    47161 taaccttttt tgataaatac ttcatagcta ttgcactaca atcatatgag gtagatgaag
    47221 ttgaatctat taagtactca gctattgtta tatcaaaata catattttct aagtttatat
    47281 tatatggttt taaagatata taatcatctt ttaaattata tccatacttt ttaatttcat
    47341 tatttgaaaa tatactatct aactttgaca cttcatttga atctacataa tatatttttt
    47401 ctccatctac acttataaaa atatatatta tatttttttc aagtatatta ccttgtcctc
    47461 taacagtttt taaaataact tgtccttttt tgtttacttc atctataaat ttttctgtat
    47521 cttttaattt taatatttct attttttctt ctacgtgttc tgtgtcatct gaatcgacca
    47581 aatcaactaa tcttgataag agacttgtaa atccaaagta tctaaattta tctaaaagtt
    47641 catctttatc ataatcgcca aaaaccatac tatctaaatc cacttctatt ggcacatctc
    47701 ttataattgt tgcaagtctt ttactttgaa tagctatttc tttattttcc tctattttct
    47761 tttttacact acctttaagc ttatctgtat tctctattaa attctctata ctagaaaact
    47821 cttttataag ctttatacct gttttttcac cgattccagg tactccaggt atattatctg
    47881 acttatctcc cataagacct tttaaatcaa taaattgtgc tggagtcatc tcgtattttt
    47941 caattacaga attaaagttg tattcttcaa cttctccaac tccttttttt gttataagag
    48001 tagttgtatt atctgaagca agttgaatag catctttgtc tccagttact atgtatacct
    48061 tatacccatt attttctgct tttttagaaa cagttcctat aatgtcgtct gcctcatacc
    48121 catctatttc caatctattt attttgaatt tatctaaaag gtcttttaat ggctgtaatt
    48181 gttctgccag ttcatctggc attccctttc ttccagcctt atactcttta tattctaaat
    48241 gtctaaatgt aggtgctttt ctgtcaaagg ctacacttat atgtgttggt ttatatatat
    48301 ctatcatttt aaatagcatt gttgtaaatc catatattgc attggtcttt aacccttctt
    48361 tattattcat ttctggcaga gcataaaagg ctctgtttat tattgaattt ccatctatta
    48421 taattaattt tttttccaaa ttatatcact cctaaaaatt atctattttt gtaacatata
    48481 tattatatac caatatagtg ttatttaata ttcccataat aataatagta tattaaaatt
    48541 aaatatgttg caaacaatat atttttttgc tataataata tacatgacat gcccgaatgg
    48601 ttgaattggt aaatacaatg gattcaaaat caactgtgtt gattcataag tttttataat
    48661 gttttacagt ttgatgagta ttttgtattc aatattcctc ttgcatattc ttggaaaatc
    48721 tggtaggata tacaagtagt ttggatttaa aatataagcc gctgtggcgg aactggcata
    48781 cgcatacgac tcaaaatcgt acgggaaacc atatgggttc gagtcccatc agcggcacca
    48841 ataaaaaaaa ctctttagta tatactaaag agttttttta ttgtaatatt ctttcatctt
    48901 tatttctagc cttgtataag tataaatacc atagtcataa cttcttgaac tcctaaaata
    48961 tatctaaaca aagtaggatt ttttatgtat tcacttaata aatccatact tcttatcata
    49021 ttgatttttt cccaatctct aaaaattttg atacaaataa aaaatacaga gaactacttg
    49081 atatactaga aggttattct gtaccatttg gtattgctgt aggtgttcca gatggaaatt
    49141 taccaaatgc tagagatgta gtttataaag tttctagagt ttaggataaa gtgttgcaac
    49201 aatcaattag ttacttgaag agttaaatca gtaatataca aataaattaa tggaagagga
    49261 agctatttaa aaagtagttt agatttttca ttgtaaaata attaaataaa gaataaggtg
    49321 ataagtattt tatttattat cttattcttt tttatttgtg gaatgataaa aataagaatt
    49381 aagtgtaata caatgtaaat aaaatgtgtt aattttaagt atattttgta aagtttataa
    49441 aatctgaaaa gctcaagata tattttcact ataatggata aaatatcaaa atattgaaat
    49501 aaacaaaaaa taatgatata ataagaaaaa ataaagaaaa aaagtaaccc ttagttgtaa
    49561 aagggttatt tttttgtgat aaaatatcca taaaaatatt cataccaaag taaattaaga
    49621 tgtatttcat ttctcaaaaa tatcctaata taaaaaataa agaaatattt ataaatttaa
    49681 ttattagaca tagataatat ctaaaaaata taccaatttc aacaaatagt aactttttta
    49741 caacaaatgg aaactatgga agcaataatt aaaataaaat ttataatcaa tcataattat
    49801 ttagtataat gattcgatat tattttaaag tatttgtcat ttaaaacaaa cttgcgataa
    49861 aagcatatct gaaagtattc tgattctaat acttaataaa gagttctttt tacagttata
    49921 gatataaaca acatctatct taatagactg tatagaggca caatcaaagg taaacacata
    49981 tagttaagta atatgtcctt gatttttctt tattataata gctttattaa ttcattttaa
    50041 tattacatca ttttaaaaaa attatatgaa aagtcaaaat ttacatatag attaattaaa
    50101 cttgtaaaaa cacatataaa ttcatacaat taagtactta aaacttgaaa atacttactt
    50161 tgctatatat tttagataaa gatataattg atttggttaa gaaaggagtc taatatgata
    50221 ttcagtagtt taatattttt atttttattc tttccagtca cactcatttg ctattacttg
    50281 gcaaatccta agtataaaaa tgttatgtta cttctagcaa gtattgcatt ctacgcatgg
    50341 ggagaaccaa aatatatttt tttgatgata ctatcaataa tagtaaacta tatatttggt
    50401 attaaagttt ctggcagtaa aaataaaaaa atgtgggttg tgctttctgt tatttacaat
    50461 atatctattc ttgctgtatt taagtatagc aattttttta tagataatat taatttttta
    50521 ttcaatacag atataactat acctgaggtt aaattacctt taggtatctc atttttcaca
    50581 ttccaaataa tgagctatgt aatagatgta tatagaaatg atgctaaagt tcaaagaaaa
    50641 ttagctaatt tagcattata cataagttta ttccctcagc ttgtagcagg tcctattgtt
    50701 agatatcaga ctgttgcaga tagcattgag tatagagaac acaattggga caaatttact
    50761 caaggtgtaa acagatttgt tgttggtctt ggaaaaaaag taattctagc aaatcaatta
    50821 ggtgtaatcg cagatggtgt atttaataag tcagctggag atttatcaag acttgaggta
    50881 tggctaggta tagtttgcta tacacttcaa atattctatg actttagtgg ctatagtgat
    50941 atggccattg gtttaggtaa gatgtttgga tttgagtttt tagaaaattt caactatcca
    51001 tatatctctc aaacagtatc tgagttttgg agaagatggc atatatcttt aggtacttgg
    51061 tttaaagact atgtgtactt cccattaggt ggaagtagag tctctaaggc aaaactatat
    51121 agaaatctat ttgtagtatg gtttttaacc gggatgtggc atggagctag ttggactttt
    51181 attatatggg gtctatatta tggaatatta atatcagtag aaaaagcatt cttagaaaaa
    51241 tttctatata agatgccaag aattgtaaga cacatatatc tacttcttat agtcatgatt
    51301 ggatgggtat tttttagagt tgatgattta ccagcagcta ttaaatttat aggtattatg
    51361 tttggtgctg gaaattatcc ggttatcagt gatgcattca atgtatttat aattgaatat
    51421 tggtatataa ttgttggggc aatccttcta tcaactccta ttgtaaaagt tgtaaaatca
    51481 ttattaatta agaaaaataa aaatacatta aatagcacat tatatcaagt tactaatgtg
    51541 gttttcattg tttcttgtat gtttattgta ataataatgt taagtagctc aacctataat
    51601 ccattcttat attttagatt ctaattggga ggtataaaca tgagtaaaaa aaaaccaagg
    51661 attgttgcca tttcattttt aattatagta tttggatttt tcatactaaa cttcacactt
    51721 ccaaagaaaa aattaagtat aactgaaaat agaaatcttg caaaatttcc gactgtagaa
    51781 aatttaaaaa aatgtgactt tatagccaag tttgaagaat attttaatga ccattttgca
    51841 tttagagatg aaatgatatc aataaataca aggactcagg ctaatttaaa taaaactaag
    51901 gtaggtaact attatctagg aaaagataac tggatattag gtatgtttcc agaaatttta
    51961 gaccaaaaac aattaagtcg atacgaaaaa tccattaatt atttatctaa tctaagtaaa
    52021 gatatgggaa aaaatgtgta ttttacaatg atgccacata aaactaatat gttaaaacat
    52081 ttgtatccta aatatgttga taatatggat aatatagaca taaatttaaa gaattttaaa
    52141 aatagattaa atccagattt aattaaatat atagatatgg atgagtattt tttaagtaac
    52201 tattcagata aagaaagaga aaatttatat tttaaaactg accatcattg gactggatat
    52261 ggagcatttg aaggctttaa aatgatggca tcaaatatgg acttaggtct atctaagcaa
    52321 gatttagaca agcattttag tagctataaa aagtctataa ttactgataa gaaatttata
    52381 ggaagttata atcaaaatat agatatgcta attaaagaaa atgaatatgt agtttatatg
    52441 acaaaagata accaagatta tagctttgat ataaatggag aagaaaaaga agaaaaagaa
    52501 gaaaaagaag tttatgcaac tgaaagagat aataaagagt gggattatgg tggtgcatac
    52561 attagaggag ctaaaactaa tatattaact ataaaaaacc ctaaagcatt agtagataaa
    52621 aaaatactaa tatttagaga ttcatatcag gctcctacaa cacttttatt tgctgatatg
    52681 tttaaagaag tagagatagc agaccctaga aatataaact caatatataa aacatacaaa
    52741 gagataatcg aggaaagtaa tgcagatatt gtaatgttta tgtataatag ttctgggttt
    52801 gattcaatga tagattcaat gatagataaa ggaataaagt agtaaataaa aagcctctat
    52861 taaattcaaa atttaataga ggctttttat tgtttatttt ttattgcttt ggtaaaaata
    52921 aactaatcaa aataaaagta aggagatgta gcatattgaa caaaagacat agtctaccta
    52981 tagtatcagt gatattattg atggtcttaa ttatctttaa taataaccaa aattatatta
    53041 caatgaaaga agtttttaat gtccatatta tgcagagtca aaataaaaag attaaacaaa
    53101 agtattatct aactaaagat ggatggctat taggcaactt tgctaaatta ttatctgatt
    53161 atcatataaa atcttactcc aaagccatta ataacttgag taagttaaat agaaaaaaag
    53221 atttttactt tgtttcactt cctcataaga caaatatgct taagcacttg tatccaagtg
    53281 gtatagaagg taaaaataat atagatatta acaaagaaaa cttagaaaag cagttaacag
    53341 acataaattt cttagatata gatgattact tttatagaag ttttaccgaa aaagagagag
    53401 aaaaattctt tttaaaaaca gaccatcacg gaaattcatt aggagcattt gaagaatata
    53461 agtttattct tgataaatta gaccttggct tatctaaatc agaaatagat agtcatttaa
    53521 aaagatacag tactcgatat gtaaatgaca aggtctttat aggtagttat aataaaaaga
    53581 tgggatatcc acttaagaaa aaagagtcta taagctatgt tgaaataagt gatgcaaatt
    53641 acaaatattt tattagtaat ggtactaaaa atactgagat taatgaaaat atcataaaag
    53701 caaaattaaa agataaaaga gaatgggact atggtggagc ttatatgaga ggttgtgatt
    53761 gtcacatact taagattaga aattcaagct ctttaactaa taaaaaggcc cttatattta
    53821 gagattcgta ccaagcacct atgacttgga tgttggctga tacttttaag gaactcgaaa
    53881 tactagatcc tagatatata aaaaatatag atatgaccta tgatgagata attaaaaaca
    53941 gtaatgcgaa tgtagtttta tttatgtata atagctttgg atttgctgga atgatacaag
    54001 aaatgattga caagggaata gcataacaat ttgaatcagc tcctttttat acaaaaaata
    54061 gttctcaata attcctaatc tatatgataa tttagtacat ctctttaaca ataaaaataa
    54121 aatcacaaaa attcttatac catcacttat ctttatgata aattggttga gaaaaaattt
    54181 tataatttat atgatatgca atcttaatta attctttatc tactgcaacc tttaataaaa
    54241 gaaaccatcc aagactatta atatgaattt atataaaaat tactttcaaa taaagtactg
    54301 cagtattttt atttaaagta tctataatct ttaaccacaa aaagccacaa aatacaatgt
    54361 atacttatgg ctttttataa atttatataa ttgtatataa agatatattc taaaactgtc
    54421 ttaagaatct aaagtttaaa aatccaatat ttttattgtg aatttaaact ttttattaca
    54481 actttttatc taatttattg tatacaacaa cactacaaat aaattgaaat aaaataagca
    54541 atccaagaac aactaaaacc cacttgctta ttaccattac agaaaataca ctcaatatag
    54601 aaacaataaa tataagaatt acaagaacat aggacatgat ttgattagtg atacgtccag
    54661 ctttatcacg taacataact cttctttcat cattcatctc tatcctttgg ttttttatct
    54721 tttcttcata atctactgct ctttcaggct tactccaata catatattga aaaatcatac
    54781 atatacctgg acctaaagca gctccagata atccccaaat aaaagcctca aatgaaaact
    54841 cagtaaacgt agccaataaa acaagtaaaa ttccacaaat aaaatatact attccaagat
    54901 ataacatatt ctttttcatt tttactcctc ctcataaata aatatttctt caatactcat
    54961 ctgaacctcc catgactaaa gtcacagggt tcctgcttca cagaattttg catactaaaa
    55021 tatgtcttac taattctcca caggccatcc ccgtagttcc tacggttctt acatacgtta
    55081 tttattttga attgttatta atcttaatcc ttcttttaat atatttatac ttgcatttat
    55141 atctctatca tgagtttcat tacaactagg acaaatccat tctcttatat ttaagtcctt
    55201 aacttcctcg tttttatacc cacatttatt gcatatttgt gaacttgcaa agaatttgcc
    55261 tacttttaca atttgtctac cataccaatt agccttgtat tttaattga
//
LOCUS       DAMKPG010000015        54919 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMKPG010000015 DAMKPG010000000
VERSION     DAMKPG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.017"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            123..1358
                     /locus_tag="PC621_000973"
     CDS             123..1358
                     /locus_tag="PC621_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF4108145.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
     gene            1434..2246
                     /locus_tag="PC621_000974"
     CDS             1434..2246
                     /locus_tag="PC621_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4108146.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(2586..3767)
                     /locus_tag="PC621_000975"
     CDS             complement(2586..3767)
                     /locus_tag="PC621_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF4108147.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(3748..3882)
                     /locus_tag="PC621_000976"
     CDS             complement(3748..3882)
                     /locus_tag="PC621_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4108148.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            complement(3882..4172)
                     /locus_tag="PC621_000977"
     CDS             complement(3882..4172)
                     /locus_tag="PC621_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108149.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            complement(4438..4668)
                     /locus_tag="PC621_000978"
     CDS             complement(4438..4668)
                     /locus_tag="PC621_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108150.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            complement(4692..4883)
                     /locus_tag="PC621_000979"
     CDS             complement(4692..4883)
                     /locus_tag="PC621_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF4108151.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            complement(4998..5138)
                     /locus_tag="PC621_000980"
     CDS             complement(4998..5138)
                     /locus_tag="PC621_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108152.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            complement(5191..6102)
                     /locus_tag="PC621_000981"
     CDS             complement(5191..6102)
                     /locus_tag="PC621_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF4108153.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            complement(6114..6812)
                     /locus_tag="PC621_000982"
     CDS             complement(6114..6812)
                     /locus_tag="PC621_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108154.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            complement(6809..7492)
                     /locus_tag="PC621_000983"
     CDS             complement(6809..7492)
                     /locus_tag="PC621_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108155.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            complement(7489..8334)
                     /locus_tag="PC621_000984"
     CDS             complement(7489..8334)
                     /locus_tag="PC621_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108156.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            complement(8438..9019)
                     /locus_tag="PC621_000985"
     CDS             complement(8438..9019)
                     /locus_tag="PC621_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4108157.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            complement(9097..9954)
                     /locus_tag="PC621_000986"
     CDS             complement(9097..9954)
                     /locus_tag="PC621_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4108158.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            complement(10167..11060)
                     /locus_tag="PC621_000987"
     CDS             complement(10167..11060)
                     /locus_tag="PC621_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4108159.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            complement(11076..12083)
                     /locus_tag="PC621_000988"
     CDS             complement(11076..12083)
                     /locus_tag="PC621_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF4108160.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            complement(12080..14215)
                     /locus_tag="PC621_000989"
     CDS             complement(12080..14215)
                     /locus_tag="PC621_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF4108161.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            complement(14215..16647)
                     /locus_tag="PC621_000990"
     CDS             complement(14215..16647)
                     /locus_tag="PC621_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108162.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            complement(16634..17023)
                     /locus_tag="PC621_000991"
     CDS             complement(16634..17023)
                     /locus_tag="PC621_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4108163.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            complement(17136..17639)
                     /locus_tag="PC621_000992"
     CDS             complement(17136..17639)
                     /locus_tag="PC621_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF4108164.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            complement(17655..18152)
                     /locus_tag="PC621_000993"
     CDS             complement(17655..18152)
                     /locus_tag="PC621_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF4108165.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            complement(18173..18361)
                     /locus_tag="PC621_000994"
     CDS             complement(18173..18361)
                     /locus_tag="PC621_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108166.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            complement(18438..19076)
                     /locus_tag="PC621_000995"
     CDS             complement(18438..19076)
                     /locus_tag="PC621_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HDF4108167.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            complement(19396..19617)
                     /locus_tag="PC621_000996"
     CDS             complement(19396..19617)
                     /locus_tag="PC621_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108168.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(19618..19752)
                     /locus_tag="PC621_000997"
     CDS             complement(19618..19752)
                     /locus_tag="PC621_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF4108169.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            complement(19759..20880)
                     /locus_tag="PC621_000998"
     CDS             complement(19759..20880)
                     /locus_tag="PC621_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDF4108170.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            complement(21160..22554)
                     /locus_tag="PC621_000999"
     CDS             complement(21160..22554)
                     /locus_tag="PC621_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108171.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            complement(22664..23191)
                     /locus_tag="PC621_001000"
     CDS             complement(22664..23191)
                     /locus_tag="PC621_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HDF4108172.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            complement(23227..23769)
                     /locus_tag="PC621_001001"
     CDS             complement(23227..23769)
                     /locus_tag="PC621_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF4108173.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            complement(23794..24252)
                     /locus_tag="PC621_001002"
     CDS             complement(23794..24252)
                     /locus_tag="PC621_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4108174.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            complement(24359..24772)
                     /locus_tag="PC621_001003"
     CDS             complement(24359..24772)
                     /locus_tag="PC621_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF4108175.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            complement(24868..25251)
                     /locus_tag="PC621_001004"
     CDS             complement(24868..25251)
                     /locus_tag="PC621_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF4108176.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            complement(25266..25589)
                     /locus_tag="PC621_001005"
     CDS             complement(25266..25589)
                     /locus_tag="PC621_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF4108177.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            complement(25814..28474)
                     /locus_tag="PC621_001006"
     CDS             complement(25814..28474)
                     /locus_tag="PC621_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDF4108178.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            29023..29640
                     /locus_tag="PC621_001007"
     CDS             29023..29640
                     /locus_tag="PC621_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF4108179.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(29797..30249)
                     /locus_tag="PC621_001008"
     CDS             complement(29797..30249)
                     /locus_tag="PC621_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HDF4108180.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            30697..32055
                     /locus_tag="PC621_001009"
     CDS             30697..32055
                     /locus_tag="PC621_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4108181.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            32328..32708
                     /locus_tag="PC621_001010"
     CDS             32328..32708
                     /locus_tag="PC621_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4108182.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            32833..33033
                     /locus_tag="PC621_001011"
     CDS             32833..33033
                     /locus_tag="PC621_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108183.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            33229..33957
                     /locus_tag="PC621_001012"
     CDS             33229..33957
                     /locus_tag="PC621_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4108184.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            33979..34896
                     /locus_tag="PC621_001013"
     CDS             33979..34896
                     /locus_tag="PC621_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF4108185.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            35038..36222
                     /locus_tag="PC621_001014"
     CDS             35038..36222
                     /locus_tag="PC621_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF4108186.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            36329..36517
                     /locus_tag="PC621_001015"
     CDS             36329..36517
                     /locus_tag="PC621_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF4108187.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            36667..37317
                     /locus_tag="PC621_001016"
     CDS             36667..37317
                     /locus_tag="PC621_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF4108188.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            37420..38358
                     /locus_tag="PC621_001017"
     CDS             37420..38358
                     /locus_tag="PC621_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF4108189.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            38384..39235
                     /locus_tag="PC621_001018"
     CDS             38384..39235
                     /locus_tag="PC621_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4108190.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(39520..40254)
                     /locus_tag="PC621_001019"
     CDS             complement(39520..40254)
                     /locus_tag="PC621_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HDF4108191.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            40380..41096
                     /locus_tag="PC621_001020"
     CDS             40380..41096
                     /locus_tag="PC621_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF4108192.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            41230..41922
                     /locus_tag="PC621_001021"
     CDS             41230..41922
                     /locus_tag="PC621_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108193.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            41912..43093
                     /locus_tag="PC621_001022"
     CDS             41912..43093
                     /locus_tag="PC621_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF4108194.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            43375..43830
                     /locus_tag="PC621_001023"
     CDS             43375..43830
                     /locus_tag="PC621_001023"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF4108195.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            43820..44545
                     /locus_tag="PC621_001024"
     CDS             43820..44545
                     /locus_tag="PC621_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF4108196.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            44517..46898
                     /locus_tag="PC621_001025"
     CDS             44517..46898
                     /locus_tag="PC621_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF4108197.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(47103..48332)
                     /locus_tag="PC621_001026"
     CDS             complement(47103..48332)
                     /locus_tag="PC621_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF4108198.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            48932..49846
                     /locus_tag="PC621_001027"
     CDS             48932..49846
                     /locus_tag="PC621_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108199.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            49870..50505
                     /locus_tag="PC621_001028"
     CDS             49870..50505
                     /locus_tag="PC621_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF4108200.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            50881..51597
                     /gene="pyrF"
                     /locus_tag="PC621_001029"
     CDS             50881..51597
                     /gene="pyrF"
                     /locus_tag="PC621_001029"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF4108201.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            51623..52666
                     /gene="carA"
                     /locus_tag="PC621_001030"
     CDS             51623..52666
                     /gene="carA"
                     /locus_tag="PC621_001030"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF4108202.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            52719..>54919
                     /gene="carB"
                     /locus_tag="PC621_001031"
     CDS             52719..>54919
                     /gene="carB"
                     /locus_tag="PC621_001031"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF4108203.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT        18775 a         8765 c         9817 g        17562 t
ORIGIN      
        1 ggggatagcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaactctgtg actttagtca tgggaggttc agaactatga attaacaaaa caattatgtt
      121 ctattggact aatatttata atgttttttg gagggtttgg aacaaactgg aatatggcta
      181 aacctgttgc tataccttct atattaatgt cttcactggg tgtaataatt acgtctgggg
      241 ttacaggtat gttttgccac atggtaatag gaactagttt acttgaaggt ttattaattg
      301 gggcaattgc agcttcaaca gatgctgcat ctgtgtttgc tgtgttgcgt tcacaaaaac
      361 taaacttaag gggttcactt gcatcattat tagaagttga aagtggaagt aatgacccaa
      421 tagcgtatat gttgacacta attcttttga cattgatgaa taattctggg gtagaactta
      481 ttgcaccaat ggtaataaaa caaatagctt ttgggttagc tataggattt atactagcaa
      541 aattatctgt ttatatttta aggcattcaa acttcgagat agaaggattt tatactatat
      601 ttataacagc tattgcaata ctttcttatg catttagcga gtatcttgga ggaaatggat
      661 atttaagtgt ttacatttca ggtattatta taggaaatag taggatacct cttaaaaaaa
      721 gtttggttca tttctttgat ggaatttcat ggattatgca aataatgtta ttctttatgt
      781 taggattgtt atctttccct tctaagttac caaatgtatt tggtatagca atggccatat
      841 ctatattcat gatagttatt gcaagaccat tagcaacatt tatagttcta gcaaaattta
      901 aattctcaaa taaagaaaaa ttattcatat cgtgggtagg tcttagaggt gcggcttcag
      961 tagtttttgc aatttatgct gttactcaag gagtagccat agagaatgac atattccata
     1021 taatattttt tatagcgttg ctttcagtag gaatacaagg ttcattgata ccaactgtag
     1081 ccagaaagtt ggatttagtt gatgataata cacctgtatt aaaaacattt aatgattatg
     1141 atggggaaat tagctctaaa cttatcgaag taaatgttga tgagggtaat aaatgggcta
     1201 ataagagtat aatagattct aatataccag acgatatatt aatagttatg ataaaaagaa
     1261 aggaacaggt tttaatacct aaaggttcaa ccattataaa aacaggggat attttggtat
     1321 tgagtggaga tagaattgaa gaattaatac aattataatt tattttgtat tgactttaac
     1381 ctaaggggaa agtttaaact taaattaatc aaatctaatg cgcaggtgat gagatgtttt
     1441 ctataggaat gttttctaaa ataaataaaa taactacaaa gacattaagg tattatgaag
     1501 atatagggtt gttaaaacca gaatatgtag atgaatttac aaaatatagg tattatacaa
     1561 cagagcaatt acctaaatta catcagataa taactttaaa acaaatgggt ttatcattag
     1621 ctgagataaa aaaagctgta gaaaatcctg aagaagttga aaatatttta atgaataaag
     1681 aaaaagaaat gttggatgta attaaaagtg aagaatataa aataattaaa ctacgtaatt
     1741 accttatgaa tttgaaagga gaagtcaata tgaatgatgt aattattaag tctttaccaa
     1801 aagttaaagt tgcttctatg agaaaagtgt taaagggata tgatgaactt tttaatctat
     1861 ttccaaatgt aatggctgaa gaaatgatga aaacagggtg tacatgtgca gttcctgatt
     1921 attgttttaa tatatatcat gatggtgaat atagagagac tgatatggat gttgaaatgt
     1981 gtcaagctat aaatgattta aaggaagata cggatatatt aaaatttaaa gaattagatg
     2041 cagttgaaaa tgcagtttgt atattacaca atggtccata ttcaacatta ggaaaaactt
     2101 atgcagatgc atttaaatgg atagaggaca ataaatataa agtggttgga aatcctagag
     2161 aatcttatat agatggtata tggaataaag agagtgaaga agactggttg acagaagttc
     2221 agataccagt gaaacgcata ttataatatg tctacaaagt ataaataaga ataaaaaagg
     2281 tagtgtctca aagtaaactt tttagtttat gagacactct ttttcatatg aaatcaagtg
     2341 tatttttata attccgatta tgaaaaaagg cttatctcta tttttggaca gcctcttatt
     2401 tttttattat aaaaacttac taaatttctg attatctttt cataagttgg tagatgccga
     2461 tatatcccgc attttcgggc tgtatgggca ttttcttttt ggaagatacc ttgcataagc
     2521 tggcatttac cagcatgaaa atgcaaccaa aagtagtaaa taagtagtaa gatacatttt
     2581 cgatattaag aagccagcct ttctaactct gcctttgcag actggaaagt accgtgagca
     2641 taatacccta atgtcatggt tatgtttgcg tgtcccatga tgtattgcag ggtgtttggg
     2701 ttcattccct tatttgccat gttcgtacag taagtatgtc tgaatgaatg gggcgtgatg
     2761 tttggtaact tgtccttatg ggtcttgttg tacttcttaa tcaacccacg caccatactt
     2821 tcataatttc ctgctacctt tggcaatccc tcacggttca agaataagaa attgctataa
     2881 ccaccaacaa caagcggttg tgcttttcct cggttcttta aaattcgctt gagtgcttga
     2941 tatgctcttt ctgttaatgg aagctctcgc ttaccgtttt ttgtcttagc tttggaaata
     3001 tagtagccca cctctgtatc acgcaatagc tggtggtcta tgttaatcac acggttaagc
     3061 atatcaatgt gtgttgtcag tccgcataat tcagagatac gaagtcccgt ttccagcaga
     3121 agaataactt catcacgata cttgctgtaa acattatctg tttccataaa ggcaaggagt
     3181 ttttcttcct gctctggtgt caggataacc tttgcttccg tatcatcttc cagaacatca
     3241 ctcaacttgg aattaaaagg attttttctg atacagtcgt cttgtatcgc catatagaat
     3301 gaagccttta gggaacgctt atagttatct atcgtcttgt aagaataacc tttgccattc
     3361 attctgatag cccattcttt agcgtcggac tgcttgaccg tatcaatcgc cctcataccc
     3421 aatgggtcat tttccagtgc gttcacaaga tactgtcgtc ctaattccgt gtttctcttg
     3481 atgttctttc tttgattgtt ctttttttcg taaagctggc agactgtcat tttaccgccc
     3541 accgtgtcga taccgtcgtc aaggtctttt tttatctgct tttctttttc cctcaacgat
     3601 atatcatcac gttttccagc aggtgtcttg tctgtgggta caagcttcca agaatataca
     3661 aactgtggct tcccgaatat atcggtatat ttataaacgt atcttccgtc ttttctctgg
     3721 ctctctccaa gacgcaaatt gcgtccttta ctgtcttgtc ttttcattta gtatctcttt
     3781 catagtttct agtgcacggt gtctgtgttt gaacacagcc tctctggtat aattcataat
     3841 atctgcaatc tctgtatcac tcatttccaa gaagtaatac atcaatacat tgttacgttt
     3901 ttttcgggta gctgtttgag agcttctgca agttcatcat caaggacttt aatatctatg
     3961 ccacacacat taaaaattgt gtagtttgtt tcgtagtcgt cccatgttgc cagactttct
     4021 accgtaatgt ctggtaattc gctaaaagat atttccttgt tttttcttcg tcttaattct
     4081 ttgcggtagt tccagataat acctttgaca acttttttca gcttacattc aaactgacac
     4141 tgaattgttt tttggaagtc agacggtttc ataatctcac ctcctttcca gctatgaatt
     4201 gaaaagtgtt tatccctctt tccgcaccat atctgtacag caaggtgtga tttgcttcca
     4261 aaaaaatgaa aataattaaa aaacttttcg ggtattaaaa aagcccgcac aaaatataaa
     4321 ttttgtacga gctactggta tagcgtattt aattgtaaat agtatgctgt taaattgggc
     4381 ttgaatatac tgaattgtat ttaaagcgtt tcatacggca agcccccttt acaacattta
     4441 aaccttaacc tttgtctttt tggtaatcgt ataaatatat tcctctggtg ttggccgctt
     4501 atttccaaag tatcgtttaa aatttgcctg cacgtctggg tctgtacttt ccatagtttt
     4561 gtctatggta tcttgtagca tagccctaaa ttcagataca gaaacacctt gagctttagc
     4621 accaactttc ataatataat tcatatctcg tcttttaagt cctatcatta gaaccaactc
     4681 cctaaaataa gttaatcttc tttttcaaaa attaaatcta tctttcgcat acagcctttt
     4741 gcgtcaattt ctgtgggtac aaaccctgcg tagtgatacc ccgtttttgc ataactgtca
     4801 ataatttctt tgtgtttatc agtacaaagg aatacaactc ctttcgcatt ataattaatt
     4861 tctacatact catatttttt cattcttcaa aactcctttc aaatacaaat ttatctttgg
     4921 atttattgac ttttacaaac tgggatttat cgttcaaatt tcataggtat atatctcata
     4981 ccatcggata attgctctta atacatcttg tcatcctcgc tattttttga acgattgtta
     5041 gaatttctcc tttggttata cttcttcata attacaattt taagtaagcc aacgacaatc
     5101 aaaaccataa caatgataat ccaccagtat tgttccataa cctacttccc ctcctttccc
     5161 tttcaatagt cttaaatcat cttccctgtc ctatggagaa tagggaagtt cattcgtatt
     5221 aacatgtacc atacaatgtt tacattgtgg aaagttgttt tcaatcgttt gatggactcc
     5281 ctcagcaatt ctatgagcgt cataaagagc taaattacca tcagctaata tttcaatatc
     5341 tacatacatt ttcgctccaa acatgcgggt ttttaattca tcaataccat caaccccttg
     5401 ctgctgaagc acaacttccc taatcttcgt ttcagtagca ttgtcacaac tatggtctac
     5461 cattttgtta atgctttctt tgaaaatgtc caaagcagct tttaagatac aaccgcaaat
     5521 cacaatgctt gcaatagggt cgagaatagc atagcctaaa cgtgctccta aaatgccaat
     5581 taaggaacca accgacgaga gtgcatcaga gcggtgatgc catgcgtcag ccataagtgc
     5641 gtcagaattt gtatgctttg cagcacttct tgtataccag tacatccact cttttataac
     5701 aatggataag actgccgccg taagtgcgat tccactagag acagaaagcg tattgtactg
     5761 tcctgaaaag attttcttga tacctgaata tcctattcca gcacccgtta tagccaatgc
     5821 aacagagaga ataatggaag ccacacattc catacgttca tgcccataag gatgttcttt
     5881 atcaggctgt ttttctgata ttttaattcc aaccatcaca acgattgtgc ttaatacatc
     5941 agaagccgag tgtacagcgt ccgacagcat ggcacttgac tttcctatat atccggcagc
     6001 aaatttaagt agcgataaac ccaggttgat gattagtgtg attttggaaa cctgaatagc
     6061 cttatttata ttttcgtttt gcagaagtct acctttttcc ataatatgcc ttcttatcct
     6121 gccagcttcc taaacagacg ttttataagg aagtagtacc agaccaaagc taccatcaat
     6181 cctatggaga aataaagaac cgcttcattc tgcatggctt tcgctaccca aaatgatgga
     6241 aggaagccaa ccgcgaactg gtagtaacta tcaataaaaa agggagctgg tattccgagt
     6301 aatgtaagtg cagctagttt tgtaactgcc ataccctcta atttattgct tgataatgtt
     6361 ataatcataa gcgaaacaat aattgcctgt actgagccga gaagcgccaa acatattgtc
     6421 attccaatgg acaacttttc taatgaaaaa agcaacagta caataaaagc gatgaccgcc
     6481 gaaataattg agggtactcc caaccttgta aagagatacc ccgacttacc aagaggggta
     6541 atcgaaaagt accgtgacac tttatcgtcg atttcctcta atgtaatcat tgcaaatgca
     6601 aagcagagca atacaggagc catgatagaa agtaataaat caaagatcgg atagtatgtt
     6661 tgcaaagaaa aatctgttat tccatcaaga aagggaatac cgaatttaat caaagctccc
     6721 atgacaaaag gcgtaaaaca agctgcaaac atcatagggt cgctcttgat ttgcttaaat
     6781 acctgtatgg tactggataa tatccttttc atagctttac acctcccaaa cttctccaca
     6841 tatgcttaac tgtatgtcgc gcaactatgt agaggataat caacgtagca attacaagta
     6901 ccatatcaaa cgatagtaaa atgctttttc cagttatgag gttcatgcaa gctgtgaacg
     6961 gaaaaaagcg gaatatatct ggtaacttta caaacagacc aacgataggt ggaacaaagc
     7021 aaacaatttc tatcggcata atcacaatga gaaattggtt caagtttgaa atttttgtag
     7081 ccgcgataat gccaagcata gtaaagattg ctgatgtcaa agctgtacca actgcgatac
     7141 caagtaaatg attgctgccg gaaacaaatc ccaatatcac cgcaatgatt gtagaaatag
     7201 caatcaatgc gatggttttt gaaagtatat actccgatac ttttacaggc gatactacca
     7261 tagcatttaa taccttttgg cttttttcta aaaggacaat cgcccccata aaaaacaatc
     7321 ccatagctgc cgggtcagag tatatcatga tagaagcaat atccgttttc caatgtcccg
     7381 tcaaagcggc aatcccacac acatataaaa ctgttaatat aaagtagata aaatagaaac
     7441 catatttcca ttggaaatgt atatcaccac aaattaaacg tcccaatctc attgaagtgt
     7501 cctccctgta atatcaacaa aaatatcatt taatgtaggc tcactactat gaatagaaag
     7561 cagacggttt tcggaaatta aactttttaa caacttatct tctgtggttc tatcaagcag
     7621 acattctccg gttttctcgc ctttatcata gtaggtatag tgtattttgg cagctcccct
     7681 tgacataatc aaattatgtg gactatccaa tgcactgact tttccggcaa caataaaggc
     7741 tacttggtca catagctctg ttgcgtcata catattgtga gtagtaatga taatcgtttt
     7801 tccgcgtttc ttttccgcaa gaataatatc tttcattaaa cgattatttg tagggtcaag
     7861 accgcttgta ggttcatcaa ggaataatat atcagggtca tgtactaacg ctttaatgaa
     7921 attcaagcga gaacgcatac ctttggaaaa gtcagcaact tttttgtccc catcattttc
     7981 taagcctacc atatgcaata attcatcaat agaccgaggt tgcttgtcat atagagaagc
     8041 aaaataggcg agattttgtc gtgctgtgaa tttctcgtag caggttgaaa attcaaagtc
     8101 tactccaata ttttcataaa accggtttgt atgctccctt atttctgtac ctgcaacttt
     8161 tacgctgcct ttataacttg tattaagccc agttaaaatt ttttgaatag ttgattttcc
     8221 agcaccagag ggtcctaaaa aaccgaaaat ttctcctctc ccaacatgga aacttacatt
     8281 ctcaacaaac ggtttgtcag tataactaaa atacagtttt tttacatcaa tcattattct
     8341 ttcctccttt aactttatga cgtttataga tatatagtca tataaactgt aaaaaatttg
     8401 tttggccact tgcctgtcaa ataaccaaac aaaattatta ctctgctact acttgctgaa
     8461 taataccatt cactaaaaga ttttgtacca tattcaattc ttcgtcctct ttgaattgtt
     8521 ccaaacagga taaaacagaa tacagggatt ttatgataac cgtatgcgga accaaaaata
     8581 caacccctaa accttccagt atctccaata tacgaatatc actgtacata tgattttcaa
     8641 aaatatctga cgatattttt ctttgcagat agtccattaa agataaatca atctgtgaaa
     8701 gaataggcga agtaatcacc cccgttaaac ttagcataat aacgtccttt gctaaatctc
     8761 tatggttctt atactttttc tttttaacag ttgtttctat acttgtaaat aactcttttt
     8821 gtaagcgaaa attgatttcc acatatagat gttctttact gggataaaaa gcatagaatg
     8881 aaccttttga tatgtttaca gcagctgcta aatcatcaac tgttactttt tttaagccgt
     8941 ataatgaaaa cagcttttct ccctctatca aaagtttact gtttatgatt tctttttctt
     9001 gttccgtaaa tcgtggcaaa ctcataaccc ccttatgact aaaatgtttt tatcgtcatg
     9061 aaaagtataa catttactga ttatataatc aatatactat ccaagatatt tatgcaaggt
     9121 agcttccaat gcgtttattg tagtgtccgt gtcctcatta aaaatcatgt gacgattaag
     9181 ctctaaagta agtaattcct ttttcggtac atgaattaaa tcaaatactt tgttggtata
     9241 atcaaatcta aaaagcggat cgcctttggc agtaattaaa ataacaggac attgtatagc
     9301 cccattctcc aagcacgata agtcagcatt aaataagctg gcgacaaatc ttaaaggata
     9361 atagggtaaa agcatagggt catttaagca atcttgtttt gcctgttcac tataaaatac
     9421 tctgtcatag tctaaataag cgtctcctgg aaccttatag ttaggcagca attttccacc
     9481 tacacgaaag cccaactgta tcagccccat aaaacggtgt gaccatgcgg ggtattttgt
     9541 taagctcata gtttctttta atgtagtcag catgatatta tgtggaaaaa cggcttttaa
     9601 ccttgtgtcc ctacccgccg ccattgcagc taaaatccca ccttgactgg aacccatgac
     9661 agctatgttt tcaccaaaat tttctattcc gtaagtcgtt gcgtcatata cattatgcag
     9721 caaatcttcc attgtataat tttttttgat tttagggctt tttccgtgtg aaagatagtg
     9781 aatgcctatt atattaaatc ctctttcact caaaccacat agaaaattct catacatgag
     9841 agggtgtgac atagtacccg gcaaaaaaac aatggtagga ctatttcttt ctgtatacca
     9901 aatagaaagt gttatcggaa cattatttga agaaatacca acttctttat acataccaca
     9961 cgcctcctat atgactaatt tagttttata gtcatattat aacttattgc aagcagaaag
    10021 tcaatatcca taaatatatg atttaagata aaattgtcaa gaaagattta attgcttaaa
    10081 atacaatgaa atcgcttgtt agtgcagcac atttgctata gaaaaggcag actatatccg
    10141 aaaatagagc ctgccaacaa aatatcttac ataataattt tccagttgct atccttatgt
    10201 aacacaagct caaactgtga tacctgcatt gcctttgtct gattatcaat gaatttcacc
    10261 gcaaccctga ctttcacatt atccctgtca tccgtaaaca taggattgac taattcggaa
    10321 tagaggtagt ccctgccgat aggttcaagc acattgccag ccacatagta ggcaagttct
    10381 ttttctgttg ctgtcggata cagcttaaag aaagtttcca aaaatgcggt agcgtcattg
    10441 acggtatctg cgtccacgct tgcgtcggct tcttgtgcct ttggctcata gtctgatttt
    10501 tcaatcgctg gtgcaatggt tgggttctgt acgataacca tatcgccgtc tttatccaca
    10561 tggactttga cggtataggt tgctttgaca ttgcttgtct gttctccctc ttttatctgc
    10621 tgatctactt cgtaggtagc gataaaatcg ttcgttccag actgttttat atcccaaatc
    10681 agtacatttg taacagtgga gcttgtcggt atatccgtcc taatggtatc aaggttcaag
    10741 tcctgcaact cctttgtcag atagttgctg attgcctgcg tccttgcttc gatagcttcc
    10801 ttgctgttgt cccatgtgta gtaggactta cagaagttct tcacaaaatt ttcaactccg
    10861 ttggtgtcat ttagtcttaa ctgaatggtt tccgtttcat gtaccgtgtg catatcaatg
    10921 gcggtaaagt tcttatacac gccaaatgat acgctggcga taagcaccac ccacagagca
    10981 atcacagatt tcttatgtgt gcctactttc atagtgcgga ctttcgtttc ttttactggt
    11041 ttttctttct ttttaaacat ggtttccaat cctttctact gttttattct tccagcacaa
    11101 atcaagtact gttgccaata agcattgttt aaatcggcat atccgatagg gtcgcctgca
    11161 tgatacatct gagtgtttcc tacatagata ccgacgtggg ttacatagct tcctgcattg
    11221 taggtactgt ggaaaaagac caaatcgcct gcctttgcct gcgatagtgg gatatgctgg
    11281 gttgcgtcat actgattttg tgcggttctc ggtaaagata cgccagcttt gttgtagcac
    11341 cactgtacca gtcccgaaca atcaaaagaa gtgttcgggt tactgccacc atatacatac
    11401 ttccaacctt gatattttaa ggcttcattc ataacctttt gtgccagctc gcccgatact
    11461 tgattgacaa caaggtactg gttcacaagc tccacataga acatatttcc atagccataa
    11521 cgccagccac cattttttgc cacggcgata gggttcgtat aggttacttt ttgaccgccc
    11581 gacttatccc ttgcgaagtt ttccgcaagg ttgaacatat gcttttttcc gtttcctgcc
    11641 acatagccaa catagccacc gccatagtta taggactgta tcacaacatt tatatcactg
    11701 ataccttgat ttttgcagga agaaagcagg gacgcaaaat atttacaccc ctgcttgatt
    11761 gagctttccg tatctagtga gttgggtggt agtcccagac tttcagagct ttgcataacg
    11821 tcctcggctg taccgccact ttccacttgt atgattacca gcaacacatt gacatactcg
    11881 gaaataccgt tttctttggc atacttttct accataggct gatgtttcaa gacttcggca
    11941 gataggttca tacctacata attagaggaa taaccattgc ccccgtcgtc gtcctcggca
    12001 ctaatcagaa ccccaaagaa taatacgaga gaaaagagga tagaaaacag actgccaaca
    12061 atagcgatat gtcttagttt catttcttct tttgtccttt cttgaatacg gttcgtttgt
    12121 tcttctcggt attctgctgt gtatgcttct gaacggttgt tttcgtaaca cggtcagtct
    12181 tgtaattctg tcttgtggtt tcttgattga cggtctttgc tgatgtatcg ccagaagata
    12241 gtggacgttc cttgacaaca tttttcttga taggttcatc attcttcacg gttgaagcta
    12301 tggcagggcg tttgacttcc tgctgttttt ctacattaac cttagaagtg gtagctggtc
    12361 tttcatgggg acgggtagct ccgcttgtcg ctgatccgtc cgttttctta cctgcctgcc
    12421 ttgattcttg tgccttttga agttccatac gcttgtcagc gatattctgt ctatgtagct
    12481 gttgcttctt taagcgtcca gactggcggt tttccttttc ctgcaccacg ccacgcttga
    12541 aatcagacac actggacttc gccttttcct tagcagagtg aaccgcatag gcagtctgtg
    12601 tcggcatatc tttgatattt tctttgacag cagttgcctt gtccttgacc ttattctttg
    12661 tatcaaggac agctcccacg gctgaacctg ctttctgccc catgctggag cttgtatttc
    12721 cacgattgtt ctttgttcca ttctggcgtg tagattttga actggcaacg acaccaccag
    12781 ccaccgcacc agcgacacca ccagcactaa cagctcttgc aagcctatgt tccatacgtc
    12841 tagccctatg tcgcatgaac agatacggtc tgcggaatat tcgccgtccc atgctttgac
    12901 tgtcgccagc gtttaaagag aacatgctca ttaaatcgcc cagcttcatg tagatacctg
    12961 caaaatacac tatctgcaag aacgccacca taaagaacgg gtagtccgtc gatatgttgt
    13021 agaacatact ggaaatacta aaggctaccg tgacaatcag tgtaatacct gcccttgtca
    13081 taatcgtatt aaacacacgg actatggctt gttttgccat gctctcatag ctcggtatca
    13141 tacttaatag aaagctgata ggcagaaaca tagcgaaaat gataaaaagt atctggctga
    13201 acaacatcat acccgtaagc aagaatacaa atatcgtgat acccaagttg aagaacaaga
    13261 ggaagaacac catacctaaa cggtttacca cctgcagtat cgtcagattg ttgttgtcgt
    13321 tgtcctcgat ttctgttttc acgacttcct ctctggtctt gccgtcctcg tcctctgggc
    13381 ttgccgatac gagagcttcg acacggtctg ccccgatttc ctctataccc gaattaccaa
    13441 attgaagcag tagccacggc tgttgtactt gtatggaaaa taggctatcc cttatcaagt
    13501 ccacgctgtc cttgcccttg ctgtcgctgt cgggaagcat gatttttgtt cccaagtcca
    13561 gtgaagcggt actgatgtct gatgaaaagt cattcacttt cttgatgtag tcaggagcat
    13621 aggcgataaa agaagccgat aatacgaaca ccacaacaaa gttgataacg gcgtgtagtg
    13681 ccttactggt ttcccgtttt atcagtcccg tataggcaac atagatacct acgactaaga
    13741 taatcagcag aagaaatccc acataaaagc ccgaagtaga aaaaccattt tgagttacgc
    13801 ctgcaagggt ctgtatgctt cttccgatac tgtctgccat atcgttgata aagtccagct
    13861 tgtaggcttc ctgcactaca tagcccgttg cattgcttag atatagtctt atcgtccaga
    13921 caaagttggt aatacagtaa agcccatatt gcacactttt tccaatcccg tccagccagt
    13981 tccacggcaa ccacgcccag ttattatctg catagaaatc aagctgataa ttcgacagcg
    14041 gatatttgga ataaaggttc tcggtgttga tagtgtcgtc cacaagtccc gttgcgtgtg
    14101 ccaccgttcc caaaagtgaa agcaggataa gggaaagtgc cacgacaaac agagccattt
    14161 tgagaaagcg gaatatcttt tttcttgtga acacactttt tatcctttct ttcatcactc
    14221 cacctcattt cgctgtatcg gcggtctggt atcaaaggcg tgcagtaatt cttcaaagac
    14281 tgggtgtatc tgcaccacgc ccacacgtcc gtataaatct tgaagcaagc attgcccgtt
    14341 ttccaaatca cgcaatcgct tctggttgtt ctcgtcctct ttgtcaatgc tgaaaaattc
    14401 aagggtctgt tttatttcgt taatatcggt gcttctaaag gcaaatttca aaccgatatt
    14461 gtttttcaga ctttcctttg acacgtcgcc agaggactgt gtaacaaaat aaacgcctgc
    14521 gttcatggca cgtccagcac gaaccagctt gttagagagc gtttcgcctt gtgccacatt
    14581 taagaacgcc catgcttcgt caaggtctac aatcttgaaa acacttctat ccgagtggat
    14641 aaaatctaac gcaaaggtac taatcacaat cagcatggaa acggacagta attcaaaggt
    14701 cgtgtcctta tcgggtagca ctaagtcggc tacttgaatg atattgagct ggttgtctaa
    14761 gctgattgca ttttgcaccg taccgtccga gaacagcaga tgtgcaaagt cgtagtccgt
    14821 gaagctgtcg atatggtcgg caatattgca agagataggt gtatcttctt ttcttaattc
    14881 ctcaatcaca tggagcaatc ctcgctggtc gctctgggta acggcacgca ctgcctttct
    14941 aagtacgggg aacttctcgc cgtcccttga agaaatgccc gtaaggaatg tgagtatgtc
    15001 gattgccaga ctttcagcgt ctttcacatt ctgcataatc acgaacgggt caagaagccc
    15061 tgcattttct ctgtcgctgg taaggttcac gatattgatt tcatgggcga tttctgggag
    15121 cgtttctttc cagttgccac gctcggattt tgggtctaag attaccgctt gaccgccaaa
    15181 cagcacagag tagtagacaa gcaggttgtt acagaatgat tttccaccgc caagactttc
    15241 tacaaaggca gaagcaagag cattagtaac cgtatctttt acgccttgac tggcaaggga
    15301 cggttgtaga taaacattgc gtcccgtatc gacagaatag ccgatataga tacccgtgtt
    15361 ttcccctaac tgctgtgtcg caccaaagcc cagcccagct agaaaatcac ttttgacata
    15421 ctgcacataa tcgttgatgt atcgcttgct ggcaggaaga aattcagagt gaagccccag
    15481 catatcgcca gcagggcgta ccagcttgac gttcaaatcg tcgtagaagt ctttgacttc
    15541 atcacagcgt cttttcaact cgtcaaggtc tggagcagat acacggataa cataagagag
    15601 cttatacata ctttctctgg actggtctaa atccgtttcc agctcgtcca cactgtctaa
    15661 tgcgtctacc acatttgagc tggtttcact gccagactgg tatgcatgat tgtctaaatc
    15721 tttcagctct tttttcttgt tgcgaaccgt cgataacgct tttcggtttt ccacgatttc
    15781 tacattcatg ctggtatcaa cgggaaaagt aaactgctgt tgctggaaat agaagatttc
    15841 actggacgga aaatcaagct cgccgacaat cgcattgaca gtaaagtagg aaacgtagct
    15901 ttcgctgtcc tcatgttcca agcgtaaatg tcgctggctt tcttccacaa ggcaacgggt
    15961 cggacgtatc aagtcataat gcgttatcag cgtttccttt ttcagtttct tcttcggtaa
    16021 gctatactca taatcttcat aggcgacacc gtccctgccg tagatgtgtt caatcagata
    16081 gccgaagtca tttttatcta agcgtcttac tttgaagcgt cggcttattt tattttccaa
    16141 cagcttttcc attttcatgt agcggttgat ttcatcattt ggcatggaaa caaagtcatt
    16201 catcagcgtg tggttcactt catggataaa ctccttgaat gtgagaaacg ccgattgctt
    16261 cacgctcttt aggttcatgc tctggtctgt taccatgagc ttgaagccaa gaaaaaagcg
    16321 gtagtccact tgattgtccc caatcataga tactaacgct tcggtctgtt cgtctatctt
    16381 ctgataggca acgtctttta gtcgtcccgt aaccagcttc tttgactgtt cttgtatgct
    16441 tcggcttgag ctttctgtgg caatctgcaa agcgtgtatc ttgccctcac gggattgtgc
    16501 gattagctgt cggaagctgt catgcacaat gaatttctgt tcagcggata gaaaactgta
    16561 attgtaaggt atcagctcat agtaggcgaa cacctcattg tccttgttcc agacaaggtt
    16621 gttgtcgata tatttaatcg ggaacatagc tcacactcct tactgccgta atagcttcat
    16681 gtagtacctg cttatccagt ttcacggctt ttcctgcata agtaatcttt ggtcgtaggg
    16741 caaaggttat ctgtgatttc aaaaagctgt acggcttctt gccgtcaaag gtcttttgcg
    16801 acatgaacca tgtaagagct acgggaatac cgaagtattt taaaaatgcc ccctcaatca
    16861 tggataaggg cggtaagtcc ccaaacagaa tgatgataaa ctcggttatc acaaaccatg
    16921 taatctgtgt aaaggtaaca ggaaagggca ggttaaagtc attgattgca tacaagactt
    16981 tttccacgtt ccagataccc gtgtagcttt taatcttttt cacgtttttg tttcaactcc
    17041 tttcgtataa tagaaaagga cagccgtttt tcagactgtc cccaatagaa aaagctgtca
    17101 gaagcaacaa tgcttgtcgc tgatcttcca gcttcttacc acggtatctc acacataccg
    17161 tatcttgttt caataaaagt tccctcaaag gataaatccc tgccgtatgc ttcatagtca
    17221 atatagttct gcaaactagg gggaatatcg ccaagtgcct gcaattcatc aatgaagtaa
    17281 taggcaacgt cggtcatatc ctcacagcca gcgtataaaa tcatatcgtc cctatggtcg
    17341 tacagttcct ccaaacttcc ataatttgat acaaattcgt ccaaactgtc cgtgatgtag
    17401 tcgggaagct cacatatcat ttcatacatt tcattgagct cttcaatcga gatatattcg
    17461 ccaatctcaa tgggaaagtt ttccgtatca tgtacggcgt attcttcgta ccgttcattc
    17521 aagccgatac gctcggcaac atcttcttcg tcaatcggaa aagagaacca gtcgccgacc
    17581 agatacccct cattgtattt cccaagatta gcgatatata cacgcatatc ctctaccata
    17641 gaaacacctc acttctacgg cagttcatag ataccgtggt ctgtttctat aaattctccg
    17701 ttatcatcaa gatactgtcc gtaggcttca taatcaaagt aacgggtcag gcgttcctcg
    17761 atggcagaaa aagcagggtc attgtccaac aaatgccttg ccacatcagc catgttttta
    17821 caccatgaat aatgtatcac atcatggcgg tgctggtgca gttcgtcaag gctggtaaag
    17881 taacacatca gctccccgta gtcctcttgt aaatcggcag gcagactttc aaaggtatgg
    17941 taaaggtcat tcagtttttc aatggtcgca tcttcgccga cttcatcagc aaagggcagt
    18001 tccttttcaa cgatatggta atcttcactg tcgataggaa cgcctatctg ttcttccagt
    18061 tcttcctcgt caatgggaag tgtgaaccat gcggaacgga tttcttcaga agtgtgtagt
    18121 cggctttcga tacagacacg catttcattc ataggttttc acgtcctttc tttcatgctt
    18181 cagctatgct agtgtaaggg acaccaaaga catagcctgc aaaatcttct aattgtttct
    18241 gcggataatc ggcaaggttc agtcgcttca tttcctgcca caccatacgt ttatgatgtg
    18301 gtaaatctga aatgtaggta cagccgatac ttgcctgcat atccgaaaat atatctttca
    18361 agcaatcact ccaatcttaa atttaggtat gaaaaaaagc agtcaatcgt atagactaac
    18421 tgctttgtat atagctgtta aattgagttt atcaattctt gtacttcatt aaaaaatctg
    18481 gataaatgga atccatctgc tacggcatga tgtatattca ttgtaagagg cattatatat
    18541 ctttcttgct ctaattcata tttgccccaa gtaataactg gtgcaagaaa ttttccctcg
    18601 tcaaaaacat gaacatcaaa atgattatat tttacccacg gtaaacaaga tacatcaaaa
    18661 aagttcaaag attgattttc cacaaatgca cgttctgttt tatacttctc cctatctaat
    18721 ataaaacgtt tatgaaattc aaatatgcta tcagaaaaat ctgtaactat ctttgtgaaa
    18781 ttttcatctt ctttatgaaa ttcagtatag gacggagaaa tataatccca cttaattaaa
    18841 ttcccattat tatcccaact atatttgaac tcatcatgtt cattaacaac ctttgaaaca
    18901 gcccatatca tagctggata aaattttaaa tcatgcttct ttgaaaattc aataaacttt
    18961 gttacatcaa tatctacggt taagctcata acaatacgca tattatcaat gtagctttga
    19021 aacaatttac ctctattcca tttattcaaa tcaatttttg tatatttata ttccatgccc
    19081 attctccttt acattttgaa atttgataaa atgtaaggct ctctggataa catttcactc
    19141 catacaagtc tattccatta agccttatat ggagataaaa caatacacaa tttcacaata
    19201 tcactcctta tcaaaaaaac tttcgtttat tattttcata tctttattat atcaactatg
    19261 tttgagccaa gcaatgaaat tcattatgct gtatttttat ctgtgtacca ttccgttacc
    19321 ccctttcccc ttagcccact cccaaagtgg gaaaggtatg tttgcgtatc cgcaaaccat
    19381 aatgaaaaag tacggctatg cgccgataat cttattgaat agttctaaga gtacgtcttt
    19441 gacaccgcct gcattgaata ccaatccaac agcaactaag gcaaccacca agaagccgat
    19501 aagttttgaa aactcacgtt taaaccctaa gtagatacca ataaccacaa tcgccataag
    19561 cactaagctc tgggcgttgc ttaaaaacca tgtatataaa ttttgcccga aattcattta
    19621 tttttcctcg ctttctttta attcaatcat gtgtcggtca gcttcctttc cgacactcat
    19681 aatacacatc agaaccacgc cgatacccat gccaagtgat accagcagta agtccttaat
    19741 caattcccac atcatcaatc actccttttc tttttctatc agttcgtcca ccgaagccgt
    19801 ctgctgtctg attatctgct tgtgcttatc tgtcagcttt gcctgctgtt caatggtctt
    19861 gagatagtcc atctgattgc cagcgtctat ctgtttcagc attttcaagg tcggtgcaac
    19921 ctgtctttgt aaccagttca gcgtcctttg cagagtgtaa ggctctggct ttgtcgtcag
    19981 cttcacgggc ggtcgtccct cgccaatgaa ccaagaccag cgggcgttgg ttttccagtc
    20041 gctttttcgc ttgttggctt ccttatcggc aaagcggata tactggttga taatagaaaa
    20101 tgccgtcagc tctgcgtcgt agtgggtcag cagctctcta atggcatagt aggcgtgctc
    20161 atttttcaaa cgtatctcaa atcggttttt aatggggact tcctcaatcg gtatccctaa
    20221 ttttgaggat tgttcataat tcttttcata gacacagaag tacacttcac tggtaaatga
    20281 accaatgtaa agcgtatgtc ccatgccgtc cttgttggct tcgttgtgct ttaccagctc
    20341 cccagaacca tagttcttga agctacggaa tttagaaata cattcctcgt tcttgcattt
    20401 ctctatcagt tctggaatat ccagtaagcc cgttctgtcg ttgatagcta ggtcgatacg
    20461 cttcatcaca ccgccatcaa ctaatgcgtc catgaggaag tcataccagc ttctttcctg
    20521 tgccagcaga tagctttcaa actgacgaca gccttttcct ttcagttcca gcaggacacc
    20581 tttttccttg tcttgtgaag tgtaaacgaa cacgtccccg atatagtaat gctcggtgta
    20641 cttgtaatgc ccgaaatctt cgtgaagcat ataatctata ttcagcttta gaatgtcctt
    20701 gataacgtgc tgtatatcca gcgtgggaaa tcggatacgc acataatcaa agagcaggaa
    20761 catgggagcg tcgggattga atttttccac cgcttccagt agtttgtgct tcatatcctc
    20821 ggtcagtgct accttcccag cttcgatacg gcttagatgt tcacggctga taccagccat
    20881 gattgcaagc cttgtctgcg atacgccata atcactgcgt tttgctttca aggtgttcag
    20941 aaaatcagta tcattcagtt gtcatacccc caatcgtgat attgttgtga ttttgtgata
    21001 ttttcaagtg attatcacaa ctagctaaaa actaagaaaa tccttattcc tagcggactt
    21061 ttcaaggttt ttgaaccagt ttccagcgta attgtgcccc tctgttagat aatgaggggc
    21121 ttatatctgc tggcgtggct aacgccacac cagcaacggc tagtccgtgc cgtcgccttt
    21181 cgcttcgcac gtcgccgtcc cgtcctgcct tacgaatgca agagagccta tggtctgcaa
    21241 aaaatcatgt cctttcggga ctaaaggcgt gtaaaactca cttatgacac ttgttcccac
    21301 atcacaatag ccacgcccct tgatacgctt ctggaaaaac tgttttttca catcagaacc
    21361 aaagagcata ccgtaaccca attcgctgat acgccccaat ccaacacgga aattgaaatt
    21421 atctctgata ccgtccgaga aatattttgc gtctggacgc tggcaggcaa caataaggaa
    21481 atatccagct tgtcgtccca acataacgat tttctttaac tggctaagta aacttacgct
    21541 ttcctttgtc cccagcattt caaaaaacgc cacatattca tcaaagataa gaaagcaggg
    21601 cggtaaacca agataggcgt agttttcgcc cgttttatag tctgggtgtt gcttcatttc
    21661 ttcgcttctt tgtaccatgc cctcataaaa ggcattgaca caatcaatca tatcttcttt
    21721 ggtatgatac acatttccca taactgtacc taagtctgca aggtcagcgt tctttgggtc
    21781 taagatgtaa aggacagcat tcgtatgtag cagagcttca ataagcgtca gcagaaagta
    21841 ggtcttaccg ccaccagtcc caccagctat cagagcgtga gggagtacgt catattccca
    21901 gacaagattt ttcatcagcc taagacagcc gttttctgct ttcacttcgt caatggaaat
    21961 acggttcgct atcatgtcat ataaaagcgt gtactcaata tagccgtcat gcaaggtctt
    22021 gtcggtcagc tcacagtaca aaccgctttc cagcttatcc tctaatcgca aaagctggtc
    22081 ttgatatttt cctaatgtga tttcacagtg aatatgaagc aatccgtttt ccatttgata
    22141 ataaatcttt ggaaaccaga cgattttttc ccttgatctg ctttgcaggt cagtaaaaaa
    22201 accgctgtct tgtacggtat ctgcttcata ccacttgttt tccagtatca ttcgtgccag
    22261 cttttgacgg tgcaggagct ttttgaaact gtcataacag aagcggtaat acagaaaagc
    22321 gaccaatgca caaataccag tcgctatcaa tatggttatg aagttgtagg gggaaagcgt
    22381 caagccattg tccaataagc tgaaatgttc ccagtcggta cgcatgagct gtttgatatt
    22441 cagtagcagg agaaccgcca caaatacaaa cagaagcgtc cctatggaaa agtggtagac
    22501 aaggttcttg tctctggctc tgatacggtg tcctttattc aaaatcattc gcataaatca
    22561 atcactccaa tcttaattta agtatgaaaa aagcagtcaa tcttaaaaga ctaaccgctt
    22621 tgtgtgtatg aatatgaagt tgttacattg tagggtatgt tcattatata tgctttgttg
    22681 ttttaactct tgattgtgca atttcttcaa atagctgttt ttcgtttagg tctttaaaaa
    22741 ttcttttggg aattaacgta tataatcgtg gtgcttgaat taagtaataa aaatcatggc
    22801 tttcccatag tgcagagtaa atgtcccact tcacttctga gtcaatgctt tgtgttttaa
    22861 atttaatgtt ttccttagaa aataccagcg tatattcctc atgatatttt gcagtttgtt
    22921 tgaatttcag tataggcatt aggatatata aatagcttcc caatgcagtt acaacaatta
    22981 tcaatccaaa tattaaaata ctaaatacgc taaaagagga gaacagcata taaacgactg
    23041 acagcaacaa aaatacagcg actaaagcaa tatcatattt atgtatagtt ttactagaaa
    23101 ttaaatattg cctttctgct tttatatatt cactttgagt atattgaaat cttaactcta
    23161 ctgtttccaa aggtattccc ccattcgtca tataaataat ctttgttaca atttcaaatt
    23221 tgaaaatcac tttttcttga aaagtttcag ttttatattt ctcctttcag caatcttctg
    23281 aaacacctcc accacataca agcattgtgc cttaaatggt agtaaggtca attcgtcatt
    23341 ggattgtatt tgtttcattg cttcttgtaa ttctgaaagt tctaataaat tgactgccac
    23401 acaaaagaaa gtctttctac gcccgtcatt atagttagaa agtaagtaag aaagaatttg
    23461 tgttttttcc tgctgttcaa gattatattg ctcaatgcct atgttttttg ctctttctaa
    23521 atctgctttt cgccgtttat gcgtgatgaa agaatcataa tcatcaatgt gttgatattt
    23581 ttcgcatgga tattgtttgc attcatagca atattcaatc tgtccatgtt caagactaca
    23641 ttttgcaatt ctgcatgatt gattaccatt gccacaaccg ccacagtaat ttcccaaaag
    23701 catagggcat agtccgcaat tcaatccgca aagagaaaat aattgatttt tgcgttcaaa
    23761 tcctttcata gtattagtct cctaactttc aatttatcgc atacaagcat ttataaattt
    23821 cataggtaca tccatacgtt ctgcaacttg ttctggtgtc attccactat ttaagaaacg
    23881 cttgcacact atcttcatgt tttctccaac ttcgataata tgtttatctg ggtcataaaa
    23941 gcggactaca cgctgtcccc atgaatgttc tataataggg tggacatatt caatatcata
    24001 ttctttcagc ctatcagcaa atttatcaaa atcttcttct tcaaaataga cttcaaaatt
    24061 gttaccacca aaagaaatat cacttgtacc gataaactct ttgtatgttt cacttgtctg
    24121 taaggctaat ccacctgtta aagttttatt tgccccaaaa tccataatga cataaagtcc
    24181 aaaaaccttt ttataaaact caactgattt atctatatca gttactacca acatagggtt
    24241 ttttaatttc atttttgcta cctccacaac ttccaatttt ctttatgtcc acatgacagc
    24301 attttcatat atccgctatt ataagaaact atccatttgc taatggtgtg gtaataaact
    24361 actttctatt tggttttctt tttatcctca aaagtttaac aaaatcgtca aacaaagttg
    24421 tatctgttgg ctcaaacatc agccattttc cgtcatggta agtttttgtt tcatcataaa
    24481 ttctctgaac ttcttttgaa taattatctc tatatgtatc gaattttaac cgttcatctt
    24541 tacctaagat aatcataaat cccacacaat tttctctagc atataaggca cataatgttt
    24601 tgccacctcg acgatactta tattcataat tccatgcttt gccaccttta ttccatagac
    24661 attccatttc atacttttca tcaatcaagg cacacaattc attccataca ctatataatg
    24721 attgtccgac caaagcagtc atttcctctg cattaggtat tttatctagc atacacattt
    24781 ctcctttata agtctaaatt aactgtttcc acatccatat tataacagtt gtttatatct
    24841 cctacaatga aaaactagtt tgtcagctta ctttttctgc tgtccctgcg gagcgttgtt
    24901 ctgcggattt ccagcttgac cgcctttgtt cttcaataca atatcatcag ctttgatgta
    24961 ccaatcaacc gtagtccctc taaagttggc atttgccacc gtatcagcta cgggattgat
    25021 taactccact tccgcattat aaggaaagtc ctttacggga acatcagcgg ggattgacac
    25081 ttgtatcatg cgttcctgca cactacattt caaatcataa gtacgacgct taatttcatc
    25141 acttgtcgtt ccgtcctcat tttctacacg cacctcatgt cgaagtgcag agaatttcaa
    25201 tactccaaaa gttttttcgt tatctaatac aattccattt gttaatctca taatcaaata
    25261 cccccttatt tgctttctgc tggtaacata tcatcagcca ttaaaatgta gtttgtaaat
    25321 ccacgtcctt tgacgttgta accctctgcg gtaatgcgtg ggttaatcag ttttactttc
    25381 tgctctggct taaaatgctt ctcgccagct tttgcaggca atacaacgat aatgtcgtcg
    25441 gctctctgaa tatccgaata caagttgtaa cttcttgata cgagagtgta acgcccattg
    25501 atacgttgct ggtctacttt gttttcccct gcaaattcca agtttccaaa agtctgtgcc
    25561 acattcggca cgatatattt taattccata ttattttacc tcgtttctta ctttaagttt
    25621 gattgatgtg tgtattctgg catttaggaa agtgccagca agcccccaga tagtaaaggg
    25681 taaataaatg ctaacgcacc cttgactatc cgtgttcttg caggtaatcg gcagacaagc
    25741 cagaataggt atagtctgcc tatctaagct atggcggaga gcgtgatttt tcttttctca
    25801 tacagctacc gccttatgat ttcttcatct tacggtgttt gaaaaagtat gtaccagcca
    25861 gcccaccagc agaaacaaac atcataattg caaaggcaag gatatttgta ttgtcgcccg
    25921 ttttgggact gtctgttttc gtaggtgtat ctggtgtggt tggcttctct ggttcttctg
    25981 gggtaggtgt ttctggtact tccttgatag taactgtctg tccgtcgtcc tcaatgtcct
    26041 tgtgttccgc aactttgata ggttcgtctg gattgcttaa atcgtataat tcctcaaaag
    26101 ttacaagctg tttaccgcca agattggaag cgtcaaaggt aaactcgatt ttaacttcca
    26161 tagtttcgct gtcagccgta aagatgtagt cattctcaat acgcttgtca ttgatgataa
    26221 gctctgcatt ttcttctttt aacatctgcc agcctttcaa ctgatatttt gtccctttgg
    26281 taagaccgtc taatttaact gtatcagtga tagttacgtc tttcccagca agaatagtct
    26341 tttcgccgtc cttgctggta gcagtagtat ggatagtgat ttctttttcg tattcatcag
    26401 tcagcgtacc taaatcaacc acaaccttat ttctggaaat gacaatctca aacggtggaa
    26461 taagtttgaa acctttgttg ctttcacaac gtaattcttc aatgatgtaa gtatcataaa
    26521 gcaacgcacc tttgctgtcg tctggttcag aagtaccaaa ccacacaccg tcctcgcttg
    26581 tttttcctgc attggtattg tgcttgtggg aagcccaatc agaagcagta gagaattgtc
    26641 cgttatcatc agttacaaca acatgacttt cgcccgtcgt cttgcttgtg atcctaaacg
    26701 gaacatctgc aagacgcttg tgcgaacctg caccaatttt tacgccctca atatcgccac
    26761 gtttaatctg gttatagata gagtgggctt cgtcagtcaa atcaacgatt tttccatttt
    26821 ctgtgatttc aaaatcaatc ggctttgcac cgtcagtcaa atatccctct ggcggttttt
    26881 gttcagacaa tctgtatttt ccgtaaggta ataggtcagc agaagtagaa gcgataccct
    26941 caatgtcggt atggattgtc tttaacactt catttttctt gtataacttg ccctcaacta
    27001 agacggcatt ctcatttaag gaaatgattt caaaggcggt atctttcaaa gtcgcaccgc
    27061 cttgagcttt cgtgtctttg gtttctaaat ctcgtttctg gattttcaca ccgccacgaa
    27121 tgactttatc agaaacatgg tattgattgc ttccagataa tacggcaagg tcgccgtcct
    27181 ctgtaatctg tgtcagatac attcctttta tctgttcttc gctaccgtca gcctgcatat
    27241 atgtaccctc taacaagtaa cccgacggac tttttgtttc ctcaacagtt agtgttccta
    27301 atggaagtac gtttttaccg tcctgcgtat agaagctgtc ccaagatact ttatatttgt
    27361 cagctaaacg agtaatgtaa tggatagcac cgtcgctgtc tttttcagcg atagtctttg
    27421 ttacccatgt tcttgtaggc tgtgctggaa gattgttttt gttgtaatat ccagcgtaga
    27481 agttccatgt aaactctgct ccctctaaag aagcgtttcc ctgcggattg gatttctgtg
    27541 tttccatgtc aatcttgaat agctcaatca aggtatctgt tacttttggt gtatcagata
    27601 cttttaaagt cgctgttttt ccaacttcca cagataaaga gtaaactgtt ttatcaattt
    27661 taaatcctgc tggtgcagaa agttccttga tatatactat agtggctctt acttcaacgg
    27721 cttctgtatt tccgctagta tcagtcgtaa gggtggtaag ctgtttcgtg cagtctttat
    27781 cagaatacac accgtatgtt gcaccagcga ttgaataaat cccgttgccg tctgtaatat
    27841 tggcattggt ggaagtcttt tgaagtttgg tatttccgac agctaacttc gcccagaatt
    27901 gccctaactc ttgaccctcg ccagagtaga tatatccgcc acagtcataa cgtcctttgt
    27961 tttctttcac aaaggctttt gctcctgcga atacttcatc ttgtacggct tttgggattt
    28021 catcatagga agctcgcaca ttatcccatt gccagtcagg ctgaacactt aatctctgcc
    28081 agacaacaag ctgtcttaat aggtaggcgt gtttactgct tattccgctg tggatgtctg
    28141 tgtactgttt cacatactca atagataagg cgacgtcagc tatctggtcg gcactcatac
    28201 gggtacttgc gtcagctttg gtcttatagc cgtttttaaa ggctgtgttg atgtcgatac
    28261 aataagcgtc ctcaccctca acggttaaat gtccctcatt aaaggtagaa ctgatagaac
    28321 cgtcattcat tacttgttct acgatactga cacgctcttt actttccgtc caatactgtg
    28381 tattagacgc atgaaccgtt gaactcggta aagcggtaac gacagttgca aaggcaagaa
    28441 agcctgtaca caatcgtttt aatatctttt tcataattct aaatgctcct ttcattttgg
    28501 tagtaaaata gccgtccact aggaacggct ggaaatagaa aaggaacgct gatattgtgc
    28561 gttccgtagt ctatgaagta ttcaattttt cttgtttcaa tactgatgtg ctgtaccgta
    28621 tctttgcata tctgggaaat aggacgtatc aaggctcatt caatcgtagt aaattagtag
    28681 tattttgatg tgtttagctc cttgataatg ccgatagata cagtgtttta gcggataatc
    28741 aaatttttag tgtgtttttc tgttaatagt tttctaaaga tgctctattt ataaattttt
    28801 atcaaagatt ttataaataa ttgtatttaa tatacaaaat atatataaat attgatgttt
    28861 tatatataaa gttgtatatt tttttaagga tataaagtaa aaaaatatac agtatcaaga
    28921 aaaaaagata ttgggtattt aaagtaatac tttatttaat tttaagtaaa tcgggtatct
    28981 acatatatag atactaattt caaagaatag gagggtattt atatggaaat attcgcaata
    29041 ggacattcaa attatccata tgataagctt attaatatga taaaaaaata tgacataaat
    29101 tgtgtagttg acattagaga aactccatat tctaaatata atattcaata caataaagag
    29161 gcatttaacg aatctcttag aaattccgga tttatatata tatacatggg aaaagaattt
    29221 ggagctaaaa gaaataataa agatgtatat acgcaagaag gttatgcaga ttttgagaaa
    29281 gtagcaaaag aagatatttt tttaaacgga atagaaagat taaaaaaggg ttgtcaaatg
    29341 ggttatagga tagttttatt aggagctatg caagaaccta taagatgtca tagaagtatt
    29401 ttggtgggaa aagtattaaa taaagaaggt ttcgatgtaa aatatataat gcatgaagga
    29461 aatttggcat atcaggaaga tatagaggaa tcattattgg ataagtattt tagtgataga
    29521 gagcaactga gtattgacaa tttattaggg agtgcattaa cgagagaaga aatgatacaa
    29581 gaaggttata agctagctaa taaagaaata ggatatagaa ctgagaaact aggtaaataa
    29641 aaatatacaa gtagataaaa tacagtactt atcttaagta aaataatatt atgaatttat
    29701 aatagctagg catgatgtgc caattaaaaa tattctagtt atttaataat atcattaaat
    29761 ttagggtatt ttttaatagc acatcatgtt gcagttttaa aaaataaaat ctctaatatt
    29821 gccataatca actgttctat taagagtagg atgactagat ataaatctaa ctgtaatttt
    29881 tgaatttaac aaacaaggat ttgaaattaa aaatactcta tctccattga caatagaagt
    29941 aatagctact ggcaaaatat ctacaaatgt atcatatctg tttttagcaa tttgataagg
    30001 ataatttcct acaaaaacaa atagatttaa atcatatata ttatcagtaa accaagccca
    30061 ttgaccataa actttgtcga tattcattgt gcctaaacca agtgtatcaa agtatgttgc
    30121 tatagttaaa taaatatttt ccatgttttc taaatatgaa acagtatatc ttctgaaacg
    30181 tatgggtgct gtggaagaaa taggataaac catatttaca tttagattat ttgcatttaa
    30241 attaaccaaa gtatcacatc ctaaaattag tctataaaat acaatattta aatggatgcg
    30301 attttgttac atctaaagtg taatatttta tatattgaaa aagtggtaaa agtttggatg
    30361 attaaataat attataaaaa atatataaaa tgtacttatt gttattgcta cacgatagtg
    30421 atacaatatt aacgaaatat agctactttt aataataacg gaaaatattt atattaaatt
    30481 aataaatatt ttaaatgtgg tatccataaa aaataataaa cataagataa ttataaagat
    30541 atactgtgtt caaatgatat taaacaagat ttatattatt tgaaagcagt atattataat
    30601 gcgaacaata caattctaaa ctttgcattg tcaatttaca gttcgataaa ctaattaaaa
    30661 attaaattta tataaagagg gaaaagagga atagggatgt ttataaggaa aaatgttata
    30721 aagttagcaa ttcctataat gatagagcag acgtttgtca tgcttttagg agtatttaat
    30781 acaataatgg caggtcatat tggggaagaa gctgtatctg cagttggaat ggttgattca
    30841 ataaacaata tatttatatc tgtatttgct gcattatctg ttggagctac tgtcgttgta
    30901 gctcaacaaa taggaaaaaa agatagtgaa aaggtgaatg aaactattaa gcaggctcta
    30961 gtgtctggag taatattatc ctttacaata acaatattga tgtgggtttt tagaactggt
    31021 gtaattaatt tattttatgg ttcagcagaa gaattggtaa aatcaaatgc aaaattatac
    31081 atagagttta cattatttac gtatccattt attgcgacac aacaaatagc aaatggtatt
    31141 ttaagagggt gtggagatgt aaagaaacct atgtatgtga ctatgtttat gaatatagta
    31201 aatgtaacat taggatatat acttatatat ggtattgatt taaatcctct aggaataaat
    31261 ttgcaaacac catcctatgg cataacaggt gcagctatat ctattgctat agctagaata
    31321 attggatgta tagtaattat aagagcttta tttaaaggaa atgaatttat atctatgggt
    31381 aaaatatttc catttaagat tgatgttgaa actcaaaagt ctatatttaa tatagggata
    31441 ccagctggag ttgaacaact actttttaat gcaggtaaat tgattgttca agtatttata
    31501 gttactatgg gaacatcagc aattgcatcc aatgcaatag gaatgtcaat tgcatccata
    31561 ataaatgtaa ttgggaatgc tttagctttg tctgctacaa ctttggtggg acagtatata
    31621 ggacgtggag atattaaagg agctaagagt actttattat atttaactaa atttgcaaca
    31681 atttgtttat ttatagtagg agctatattt ataccaattg caccctggat ttcaagtatg
    31741 tatactcaaa atgctagtgt aattgacatc tcaacacaat taattcgaag tgattgttta
    31801 gctatggtaa tatggcctat atcctttgta ttatcagcag ggctaaaggg agccggtgat
    31861 actagatata caatgatgac tgcagtaata ggtatgtgga tttttagaat ttttacaggt
    31921 tacgtattgg gggttgtatt agaatttgga gtgttaggaa tttggatagg tatgtatact
    31981 gattggcttg ttagagggac tttatattgt ttaagattaa gaggaacaaa gtggctaaaa
    32041 cacaaagttg catagaatat atgaaatcac ttttagatgg ataactctat ttagaagtga
    32101 ttttttatgt agttattatc ataattataa gtatgatata taaggttatt aaattacata
    32161 aataaaggct ataatagtat ggatattata aattatatat tgagcagaat tacataattt
    32221 ggcgataaat attagaaata tatatctata gaaaataata tatatgggta tattattaaa
    32281 tataaaaaat attaggtatt tatttaataa taaaggtggg tattgttatg ataaataaaa
    32341 taggaaaaat aactctctat gtaaataatc aagaggatgc aaagttgttt tggacagaaa
    32401 aattgaactt tgtagtaact tatgaaaagg aaatgggtcc aaatatgaaa tgggtggaag
    32461 tagcaccaag taaaaatgaa tttacaacat ttgtgttgta tgataaaaat ctaatgatga
    32521 agcaaaacaa agagataaat gtatctcatc catcaataat tttaagtaca agagatatta
    32581 atggaactta tgaacaaatg gcaaataacg aggttgaagt tggggaatta atggagatgc
    32641 catatggaag gatgttttct tttaaagacc aagatggaaa tgattattta gtcagagagg
    32701 ataaataatt ttacaaagta ttaaatttac tcaatatctg atataattag agacaataat
    32761 tattataaac tagtctagga ttagaattat ataattaaaa attaagtgta aattttgagt
    32821 agaggtgata aaatgtttaa gaaaatggca gtactaaaag atatagcaac taaaataggt
    32881 cgtaaaaaag cgtatgaact attagaaatg gttgaaggta atgatgcctt tgtagctgag
    32941 gtaaagataa aaaagaatgg aatagaatct aaaaaagaag aaattatgtt aaaagataat
    33001 caaaaaataa tattagagta tatagaaggt taaattttaa atacatataa gttgatatac
    33061 taaatttctg agatgagttt acataaaact catctttttt acttttcaat aactttgttt
    33121 tattatttaa tatacatttt atctgcttag agttgctgat atataaaaat tactgatata
    33181 ataaaagaat ggatattaat gtaatatttt atagaaaaga ggggtattat gcttgaaata
    33241 aaaaatcttt cctttaatgt tgagtctaac aatgaggaac taggaataat aaatgatgtt
    33301 agcctttcat ttgagagggg aaaacttatt gttatcacag gtccaaatgg tggaggaaaa
    33361 tctacaattg ctaagcttat tatgggaatt gaaaaagcta cgagtggtca gataatttta
    33421 gatggagaag atataacaaa tcttagtatt acagaaagag caaaaaaagg aataggatac
    33481 gcatttcaac aaccaccaag aataaaaggt atgacagttg aaaatttact tacacttgct
    33541 catggaaaac cattgtctac tgatgtatgt tgccaatacc ttacagatgt tggattgtgt
    33601 tcaaaagact atttaaatcg tgaagttgat aactctcttt ctggagggga aatgaaaaga
    33661 attgagatag caactctttt tgctagagat ttaaaggttt ctatatttga tgaacctgaa
    33721 gcagggatag acctttggag ctttggaaaa ctcaatgaaa gttttaagaa aatacatgaa
    33781 gagtctaatc agactataat cataatttct caccaagaaa gaatattaga gttagctgat
    33841 gaaataattg ttctacaaga tggttcagta aaaagccatg gaacaaaaga ggctatatta
    33901 cctgaaatca tgtgtcaagt taattcaagt tgtgaattaa tgaaagacat gaattaatta
    33961 gaaaggaagg caaattaaat gaacaaaata gatttaaatc ttttagaaaa agttgctgaa
    34021 cttcgtggaa ctccattagg agcatacaat atacgtaaaa atgggcaagg agtagagaga
    34081 cattcaactg aaaatgtaac tataattcct aaaactgata aacctggaat agatattata
    34141 gtaaagccaa atacaaaagg agaaaatgta catataccag taatagtaac agaggctggt
    34201 gtacaggata tggtatataa tgattttgaa attggagaaa atgcagatgt tgttataatt
    34261 gctgggtgtg gtatacataa ctgtggttgt gatgatagtg aacataatgg tatacataga
    34321 tttttcttac gtaaaggagc tcgcctaaaa tatgtagaaa aacattatgg tgaaggtgat
    34381 ggagaaggaa agagaatttt aaatccagtt actattgtca atatggatga ggattcatat
    34441 tgtgaaatgg aaacaattca aattaagggt gtagattcaa ctaaaagaga aatggatgca
    34501 aacctaggta aaggagctaa aatagttgta tttgagaaat tattaactca tggtaatcaa
    34561 gttgcagaat ctaatatgaa ggttatctta gagggtgaaa actcttctgc tcaagtagtt
    34621 tctcgtacag ttgggcaaga taattctaag caaaagttta atccttgtgt tattggggat
    34681 tccctttgtt ctgcacatgt tcaatgtgat tcaataatta tgggaaattc tcaaattagt
    34741 gctatacctg aaattgctgc aaatcatcca gatgctcttt tagtacatga agcagctatt
    34801 ggaaagattg caggagacca aatactaaag atgatgacat taggtcttac tgaagaagaa
    34861 gccgagcaac aaatacttaa ttgcttctta aagtaaatta aagattttat ttaatttaat
    34921 ataattaata tagtataata tagtgaataa gtctaaaata tttttatttt taggcttatt
    34981 ttctttatgt aagaaaagct attagagaaa ttatattgta attaggatgt ggaataaatg
    35041 aaaaatttac ttataagaaa tgggacaata atatctccaa cagatggtat caaatttgaa
    35101 aaaagagata tcttggtttt acatgggaaa atatatgcta ttggattgaa ccttaaagag
    35161 aaaataagaa aagaaaaatt acttacaaat aaagagttcg agataataaa tgccgataat
    35221 atgtatattt ccccaggatt tatagatgtt cacacccatt gttataagag aaaattacct
    35281 acaggtatgg attctgattc aattggaatt gaaaaaggtt ctacagttat atttgatgca
    35341 ggtacagctg gtccctcaaa ctactatgat tttaaaaaaa agtatatgga tgagtgtaaa
    35401 acagaagtat attctttttt aaatgttaca aatgagggac taaatatatt gaatgaatca
    35461 acttctttag atgttataaa ttttgacaag gttgagtcaa ttgtagaaaa aaatatagaa
    35521 aagataaagg gtataaaagt aaaagcgagt aaatttcaat ctaatgaaag tggtatagat
    35581 ataattaaga aatcaaagca agttgctaaa aaattaaatt tacccttagt ggtatatata
    35641 ggagagtatc catcatatat agatgaagta atgaatattt tgggaaaagg agatgtggtt
    35701 acaccagtat atggaaatag tacaaatgga atccttaaag aatctggcac attaagaaaa
    35761 tcagtaataa atgcaaagaa tagaggagta ttgttcgatg tatgtcatgg agtggaccaa
    35821 tttgatttta aagtttttaa aaaagcaata aagcaaggtt tagagcctga cctaataagt
    35881 actgatatgc atataaaaaa catgaaagac gtaaactatt ctcttttaaa tgttataaat
    35941 aaaattatgg aacttgggat atccctagaa aagtgtatag aaaaagtaac tgaataccca
    36001 acaaagattt tcgatttaca tggaaataaa ggcagaataa agattggagg agaaggtgat
    36061 tttacaatat tcacaatgga ggagtgtaat gatattataa aagactacaa taataatgaa
    36121 cttgttttaa ataaaaaatt aagattgcaa tatactgtaa aatcatggat gaaaagcagt
    36181 gaagtttata gacatggata cgaaaatact ataataaact aaacaatagt gataaaataa
    36241 taaatatatg tttaaattgg tatatattgg ataaatatct aaggaaacaa caattaaaaa
    36301 ataataattt taggggaggt attttagtat gaaagcattt gttgataagg atgtatgtgt
    36361 agcttgtgga gcatgtatag gaacatgtcc agacgttttt agtatgagcg atgaagggca
    36421 ttcagtagca attgaaggag aaataccagc agatttacaa gatttatgta aagaagccga
    36481 agaaggatgt ccagtttcag ctataacagt taaatagagc tgtttaaatt tatttttaaa
    36541 ataaatatat aagatttaat tgggtactta agctatagta ttagaaattt ctattactat
    36601 agctttttaa aatagataaa cttaatatgt aagatattac tttttacaaa tatggaggac
    36661 tgaaatatga aaaaaaatta tgaaatagta ttattcgatt tagatggaac acttacagac
    36721 cctaaagaag gaataacaaa gtctattcag tattctttaa atagctttgg cataaaagaa
    36781 gatttggaaa acttagacca atttatagga ccaccattac atgatacttt caaggagtat
    36841 tataaatttg aagataaaaa agctaaagaa gctgttgaaa agtatagaga atattttgct
    36901 gataaaggga tatttgaaaa taaaatttat gagaatatga aagagatatt agaaatgctc
    36961 tataaaaatg gtaaaatatt attagtagca acttctaagc caacagtttt tgcagaaact
    37021 atacttagat attttgatat agatagatat tttaaatata tagcaggtag caatcttgat
    37081 ggtacaagag tgaataaaaa tgaagttata caatatgtac tagacctatg taatgtaaaa
    37141 gataaagata aagttattat ggttggagat agaaagtatg acataattgg agctaaaaaa
    37201 ataggtatag attctattgg agtattgtat gggtatggtt cttttgaaga gcttagtgaa
    37261 agtgaaccta cttatattgt cgaaaatgta gagtctataa aagatatttt gttataaata
    37321 tcttttatag acaaaaatat tacaaaatgg tgtcgcatat ttaaaaatat gatattatag
    37381 ttaaggaagt catttaaaaa agaggaggat gcacttaaaa tgaaatctat tttaattatg
    37441 ggtggaagcg acttcatagg ttctgcactt gctaagcgct tgattaaatg tgggtatcaa
    37501 attgatatac ttacaaatgg taaaaaggaa atagattata atggttttaa agaacatcta
    37561 atttgtgata gaaaagttcg aaaagatatg gaaaacatta tcacaggaag aaaatatgat
    37621 tacatatatg atatgacagc atatacaaaa gaagatgtaa gtaatctgat tgattttata
    37681 agcatggata acttaaaaaa gtatatagta ttgtcagcag gagcagtata taaagatagt
    37741 ggaagaaata taaaagaaga aaatgagaaa ggtgaaaatg aaaactgggg aaaatatggg
    37801 ttaaataaaa aagaggctga agattttatt ataaatagcc ctataccata tataattatt
    37861 agaccaactt acatatatgg tgaaaataat aatttatata gagagtatta tttctttgaa
    37921 aaaatagaaa aaaatgaaaa gataccagta ccaaaaggaa aacaagttag taatcaattt
    37981 atatatattg gggatttggt aaaggtattg gaaagcataa tgaaaaatcc tcatgtaaga
    38041 gaggcctata atgtgacaaa tccacagtta atttcttggg atgaccttat atatacatgt
    38101 ggagaaatta ttggcaaaga acctataata aaatatgtag atatggaaaa agtagagttt
    38161 agagaaagaa cgtatttccc atttagaaat atagacttta atcttgatat aaataagctt
    38221 attgaacatg ggctttatat accaaatgtg ttactaaaag aaggtcttac agcaacatat
    38281 aaatggtttt cagcaaataa gccaaaaatg catgacagaa aaatgaacaa agttgagtct
    38341 gtaatgcaaa tagtttaaaa aaataaataa gtaggtagat attttgaact caaaaattaa
    38401 gtatttttta aaatttttta tcttaatatt tattttgatt tttatatact cagttgcgat
    38461 agagccgagt ctattaattg tgaaaaaata taatataagt gaaaatggag cattgataaa
    38521 aaatgataca attgagagta aatctgaaag tgtaaaagta gttcaaattt cagatactca
    38581 aataggtagt ttttattcaa ctaagaatct caaaaaagta gctaataaga ttaacacttt
    38641 gaatccagat ataatagtat ttactggtga tttaattgat tactccaata aaaatccaag
    38701 tgtagatgaa attacaacta ttttatcaag tatgaatgct aggttgggaa aattttcagt
    38761 atttggcaat catgattata tgtataaact tcctaggtat tataggcaga ttatgaaaaa
    38821 ttccaacttc aatcttttag taaatgaaaa taaaaaaata aaattaaaag atgataaata
    38881 tattaatata ttaggtgttg atgaaatatt aaatggaaat cctaatataa aatatctgga
    38941 gagtcagata gataataaaa attttaattt attgttagca catgaaccag atttagtaga
    39001 tatgcttagt aaagatacaa tgaatttagt tctatctggg catagtcatg gtggtcaaat
    39061 aagattacct attaagggag cattagttac tcctccatat gggaggaaat atactaaagg
    39121 gttttatgat ataaatggaa atcatttata tgtgagttcg ggtcttggaa gtacaaagtt
    39181 accatttaga ttttttaata taccagagat aatagaattt aatattataa tctaacataa
    39241 aatatttaga aaatataatt ttgcttttta taatatgtag atgtatatac tgtaaaaatg
    39301 gattaaacca gtagattgaa tgaattaata tattaaatta atatataaaa tatttaagtt
    39361 ataattaatg tattagacaa aataaatact attaagaact attaaaatat agattaaaga
    39421 taataacata taataaataa aaaagtaaga ttgtgtagat tatataaaga ttatttataa
    39481 ttaatacaat cttgcttttt tatttaaaaa ttttaattat tatttaaaat tttttatctt
    39541 ctctttatcc catataaaca atttctcaga attatttaaa gcgccaaata catttctttt
    39601 aaaatcaggc atctctttat ttaaattagc aataagattt tttatgtact ctctcctagt
    39661 atgaccattt gcagggcagg gatttgtgat aataggatag ttagaatctt ttgaaatttt
    39721 cttagtcata tactcttcta tgtaaatcat aggtcttata actgttaaat cctgtctatc
    39781 taggtatgtc tttggggaaa aacaatttac tctaccctca taaaacatag acattaaaaa
    39841 tgtctcaatt gcatcatctt tatgatgacc aagagctact ttattacatc caagtttttt
    39901 agcattatcg ttcaaagcac ctcttcttaa attagcacat aatgcacaag gatttttttc
    39961 ttgtcttata tcaaatacaa tttcttttat gtcagtttga aattcataaa aaggaacatt
    40021 tatttcttta cacaatttat ctagtggaga attatcaaca cctccagtat ttaatgttat
    40081 ggctatcaat tcaaagtttt gtggagaaaa tctttgataa cttttaagta tatgaagtag
    40141 agttaaacta tcttttccac cagaaagtcc aaccgcaatt ttatcatttt cttgtatcat
    40201 atcaaaatct tgtatagcct tacgagcttt acttagaagt ttttgtatag acattttatc
    40261 accatccttt aaaaatatta aatttaagat ttatttaaga tttacttata aaatacttaa
    40321 gtcttatact ctaaaattaa cattatagac aaacataaca aaaaggagaa gatttaatta
    40381 tgaaaaaatt taaattaata ggagccatta ttttagtagt atttataagt gtatttatat
    40441 ctaaagatag attacttaag aaagataaag atccaatagt agctgctcaa catgattcag
    40501 aacaagatgt tgaggcaaat tctcaaaata aaaatcactc aaatagtata tcaaatatat
    40561 tattggtaaa caagacaaat ggaattagca aaaactatac tcctgaaaat attactaagg
    40621 taaacatacc ttttgttgaa gaagcaacag aggaagaaaa acaaatggca ggagaacctg
    40681 caaaggcagt tgaagattta gttaagcaag ctaattcaga aggaatacag tttttagggt
    40741 cttcagcata cagatcttat gatactcaac tggatactta tacaagaagg gtaaaatctc
    40801 agggacgaga aaaggcagat gcatatgtag caaagcctgg ttacagtgag caccaaactg
    40861 gactttgtat tgatttaaca aatccagaaa gatggtttgt tggaagtaca aaagaagcta
    40921 aatggttggc agaaaatgct cacaagtttg gatttataat aagatatcct gaaggtaaag
    40981 aagatataac aggtacagca tatgaaccat ggcatattag atatgttggt aaggatgctg
    41041 ccgaggaaat ttattcaaaa ggattaactt tagaagaata tttacaaaat aaataatata
    41101 aatgtgtaaa taatttgtat tatattggaa atttagaaat gaataaattt atagattaca
    41161 ctaactaaaa tatagtataa ttttatatga ataaagctat atacaaagat taagttagga
    41221 tgtgtaaaaa tgaataatac agttcttgtt gtggatgatg aaaaggaaat aagagacctt
    41281 atagagatat atcttttaaa tagtggatat aatgtaatta aagctgaaga tggaaaagag
    41341 gctctagata ttttaaaaaa ggaaaatatt catttaatga tattggatat aatgatgcct
    41401 aaaatggatg ggatagaagt ttgtagaaaa gttagagaaa gtttgaatat accaatactt
    41461 atgttaagtg caaaatcaga agatatggat aaaatacaag ggataatgac tggtgcagat
    41521 gattatttga caaaaccatt taacccactt gaacttactg tgagagtgaa atcactgatt
    41581 agaagagcat attattttag tggtgctaat aatgaagata aagatgtcat aaaggttgga
    41641 gtagtaatta tagataagga aaaacatagt gtaacagtgg ctggaaatga aataattttg
    41701 acatcaagag aatttgatat attatattta ctagcaaata ataaaggcag agtatatagt
    41761 acagaagaaa tatttgaaaa ggtatggaaa gagaaatatt accaatctaa taatactgta
    41821 atggttcata tgagcagaat aagggataaa attgaaaaat atacagatgg agaaaaaata
    41881 attcatactg tatggggagt aggatataaa attgaaaaat aaaagtttat taaatcatat
    41941 gtttcgaccc atattagatc ttataataag ctgtgcttta acttcaatgc ttattttaac
    42001 aagtatgtat ataataaaat tattatattt caatagtgag atatctgtaa ttaatcaact
    42061 tgtatatcag ttaagagatt tcagaagtct tataggagag agtttatttt atccattttt
    42121 atttacaatc acatcattag caatttattc catgatatcg tacaagagaa ataagcaaat
    42181 tgtagatttt ataagaaaga ctaatagaaa aaatcaaaaa ttagaaatca ttaaaagtgc
    42241 tgtaaattta atggatgaag gtaacttaga aaaaagtata gatattgagt atgatttaga
    42301 tatattaaag gataaagaaa aggatgatat tgatgaacta gcacataaaa tcaataatat
    42361 agtccatcaa cttagaaata taactattga agagagacaa gctcagcaga caaaaacaga
    42421 tttaataaca aatgtgtcac atgacttaag aacacctttg acatccataa tgggttattt
    42481 aggagtgata gaagaaggta agtataaaga tgaagttcag atgatgtatt atgttgatat
    42541 tgcatatgaa aaatcgaaaa atttaaatgt tcttataaat gatttgttcg aattgactaa
    42601 aatgcaaaat aatacaataa aactaaacaa aatagaaata aatttagtag agttattaag
    42661 tcaagtagta tctcaatttg aaatttattt taaacaagaa tttatggtta gtagaattaa
    42721 ttttaatgaa gaaaaattaa ttgtgaaagc tgatccaaat aaattggtaa gagcatttga
    42781 aaatcttata actaatgctg tgaaatatgg aaaagatggg cattatgtag atatagtcac
    42841 agagaaaatg gaagatatgg cggttgtaaa agtgataaat tatggagaac caattccagt
    42901 gttagattta ccaaatatat ttgatagatt ttatagagtt gaaaaatcga gaaatagaaa
    42961 tgttggaggt tctggattgg gtcttgcgat tacaaaaaat attgtaaatt tacatgatgg
    43021 agaaattact gtttcaagcg ataaatatca aactgtgttt gaggtacaac tgccaataat
    43081 acaatcagct tagttataaa ataaaagtac gattatgtga tataaatatt ataacaataa
    43141 agtgtttaca aaaataaaaa agtgatatat aatttaagtc agtttaataa gatacttatc
    43201 aagaattagg ggagagagtt agctcagtga tcctatacca accttttatc aggtggtcct
    43261 gctaacaacg atgagatgac ttgaaataat atactcttct tatcaataat agataggaag
    43321 agtttttttt agaattaatg taacaaactt taaagtataa tggaggatta aataatggaa
    43381 aaagtagaaa gttttaagct agaccataca aaagtaaaag ctccttttgt aagaaagtgt
    43441 agtgtacttg atggagtaaa gggagataaa gtaactaaat ttgacttgag atttttacag
    43501 ccaaatgtag aaagttttgg aactgctgct atgcacggtt tagagcatct tttagctact
    43561 tatttaagag atacattaga tggtgtaata gacttatctc caatgggatg tagaacagga
    43621 ttttatttaa tactatgggg agatgtagat gccaaaacag ttaagattgg tttagaagaa
    43681 gcattaaaaa aagttcttga atcagataaa atgccagcag ctacagccat agaatgtgga
    43741 aactacagag acttatcttt atttggggca aaggaatatg caaaagatgt tttagataaa
    43801 ggattttctt taaatatata tggagaataa tttaattgga agagaagata taagcataaa
    43861 tcaaagtgaa gttttaagat atttacaata cagaggtcaa gagatagata aagaccttaa
    43921 atatactatt gatgaatgtg tagctataac taaaaataaa ataaatccta gatatttatc
    43981 taggatttat cctattagaa taaataaaga taatgttgta gaattagagg gaacaaattt
    44041 aacattagag agtaaagatg tatatgaact tttaaaagaa tgtaatgagt gtgtaattat
    44101 ggcagctact atagggatag atatagaaaa agaaataaga cgacactctt attcaaattt
    44161 aacaaaagga ctaataattg attcatgtgg aacaacagca atagaagaag tgtgtgacat
    44221 tgttcaaaat aatttagaaa aggttttttt aaaccaagat aaatttataa ctatgcgata
    44281 tagcccagga tatggagatt taccaataga aaaaaatata gatatattaa acgttttgaa
    44341 tgctcaaaaa caaataggtc taacaattac ttctaatgga attatgatac cgagaaagtc
    44401 agtagtggct attataggta tttctaatac aagaataaat aaatctaaga aagaaaagtc
    44461 gtgtgaaaat tgtaaaaact atagaaactg tacatataga aaaggagcga actcttgtgg
    44521 aaataagaca atatctgaaa aataatatat taatatttga tggtgcaatg ggaacaatgc
    44581 ttcaacaaaa agggttaaag ttaggtgaaa atccagaagt atttggtctt caaaatccag
    44641 ataaacttat agaaatacat acagcatact tagaggcagg ttcgaatgta attcttacaa
    44701 atacttttgg atgtaatgaa ctaaaattag atagtaagta tactgtagaa gaggtgattg
    44761 acaatgctgt tttggtggct agaaaagcta tagaaaatgt agataatact aaaccaagat
    44821 atgtagcttt agatattggt cctataggag aaatgctaga gcctatgggt actttaagtt
    44881 ttgataatgc atatgagata tttaaaagac aagtactaca aggcgttaaa tctggagttg
    44941 atgtaatagt aatagagact atgatggatt tatatgaagc taaagtagct gtattagcag
    45001 caaaagaaaa ctctgattta cctattttct gtactatgac atttgatgaa ggtggaagaa
    45061 gttttactgg atgtatgcca gaatgtatgg tggctactat agaaggtcta ggtgtagatg
    45121 caataggagt taattgttca ttaggaccta aacaactttt accaatagtt gaaaaaatag
    45181 catcaagagc tacagtccct gttatggttc aagcaaatgc aggtcttcca aatatagttg
    45241 atggagaggc tatatatgat gttgatgcaa aagaattttt tgaaggggtt aaaaagttcg
    45301 ttgaagttgg tgctactata ataggtggat gttgtggtac gaatccaagt tttataaaag
    45361 aaatatctga gaatataaat tctgttacaa agggatgtat cgagaaaatt gataaatgtg
    45421 tagtctgttc accatcaaaa tttgtggaag tacaatcgcc tacagttgtt ggagaaagat
    45481 tgaatcccac aggtagaaaa tctctacaag aagcattaaa aaatgaaaat gttgattatg
    45541 ctataaattt aggcttagaa caagtaaatg ctggagcaca aatacttgga gtaaatgttg
    45601 gtcttccaga gattgatgaa aaaaaactaa tgccaaaatt aataagagag attcaagcag
    45661 tagtagatac acctttacaa gttgattctt ccaatgtaga ggccttagag caaggactta
    45721 gatattataa tggtagaaca atagtaaact ctgtaaatgg caaagaggag tctttagaaa
    45781 gtatattacc aatagttaaa aagtatggtt catgtgtggt tggacttaca cttgatgaaa
    45841 aggggatacc taaaaaggct gaagaaagat ttgaaatagc aaaaaggata gtcaatagag
    45901 cagtttcttg tgggataaaa ccaaaagata tatttataga ctgtttatca ttaactgttt
    45961 cagcacaaca agaagaagca atagaaacta taaaagctat aacaatggta aagacactag
    46021 gagtaaaaac tatattaggt gtttcaaata tttcatttgg tcttccaaat aggaaagcac
    46081 ttaatgcttc ttttctaaca ttagctttag gggcagggct tgatttagca attataaatc
    46141 caaatgaata tagtatgatg gaagctatca attcttttaa aatattaaat aacacagata
    46201 aaggatgtat aaactatata aatcaatata gtaatataaa taattcaaaa aaatcggatt
    46261 catctacaaa gattgacaaa gatttgccat tggatatttt agttgaaaga ggattaaaag
    46321 atgaagcaaa aaatgtaaca ctgaatcttt taaaagagaa ggatgaaaat tacatattag
    46381 atgagatttt aattcctgct cttgataaag taggaaaaag atatgatagc ggagatatat
    46441 ttttaccaca attgattcaa tcagcagaaa cggtaaaagt ttcattaaat acaataaagg
    46501 aaactttatt aagtaaaagt agcaataatg tatccaaagg aaaaataata gttgctacag
    46561 taaaaggtga tatccatgat attgggaaaa atatagtaaa aataatgcta gaaaattatg
    46621 gatatgaggt tatagattta ggtaaagatg tccctattga agaagtagtg gaagttgcta
    46681 aaaagagaaa tataaaatta gtaggattaa gtgcacttat gactacgact gttcaaagta
    46741 tgaaagatac aattcaggca ctgagagata atgagataaa tgcaaaggta tttgtaggag
    46801 gtgcagtttt gacagaagaa tacgctgaag aaatgggcgc tgattattat tcaaaggatg
    46861 caaagtctgc tgttgaaata gcaaagttaa atttttagca tataaattaa aataaaggtt
    46921 ttactaaata ggatatttta tatataatat aaatataagt tttagatact tatgtttaaa
    46981 acaataggaa ttaatctata ataatgagat taattaatat aaaaaatatt tatcatcaac
    47041 attaaaaagg gctgggaagt ctggtataac ttccattagc cctttttagt taaaatcata
    47101 tgttaaaaag cccaattacc atttttaaaa atctgtattt gttttccatc ataagtctca
    47161 ccaataatat ccatatcagc agaaccaatc ataaaatcta catgtgttaa actgtcattt
    47221 acaccatgtt catctaattc ttcatctttt aaattgtttc caccttccag acaagtctta
    47281 taagcagaac caattgcaaa gtggcaagaa gcattttcat catatagagt attataaaaa
    47341 attatatccg tattggatat tggtgagttg tatggaacaa gagcaacttc tcctaaataa
    47401 tgagagcctt catccgtttc aataagtttt tctagtgtat ctaatccctt ttcagcagag
    47461 aaatctatta ttttaccatc tttaaatgtt aagaagaaat tgtcaattat gtttccacca
    47521 taaacaagag gttttgtact ataaacagtc ccatttactt taaacttatg tggcattcca
    47581 aaaatctctt cagttggaat attcggatta aaacttattc cattagggtc tttggatgct
    47641 ccacttagcc aaacatgacc ctttggcaag tctagagtca aatcagtttt tgaggattta
    47701 tattttaaag ttttaaagtt atttttattt agaaagtcca ttttagattt aagattttca
    47761 ttatgttctt cccatgcttt tatagggtcc tgaccatcta ctcttgaaca tttaaatata
    47821 gcatcccaca gtttttctat tgctttatct tctgataaat ttggaaacac tttttttgcc
    47881 caatcttttg ttggcacaga gactatactc catttacatt tatcagttag agtgtaagaa
    47941 cgccattctt ttaaagcttt tccagatgcc ttctgaaaat ttgcaactct ttcagggtca
    48001 acatctttta ataaatctgg attttgggca tagactgata aaaatgcccc accttctttt
    48061 gctatatcta catattgttc tgctgtccat ttggggaact catgaaatgc ttcatctgga
    48121 gcatacaagt atttaataag agtactttct tcatcagacc actctatata aacattttta
    48181 gcaccacatt tatatgcttc tttaagtgca tttcttgcaa aatcagcaca ttctattggt
    48241 gactttataa gtagaatttg acctggctgt atgttaactc caacttttat tgctaattca
    48301 gcgtattttt ctaaatttct atttaaattc atatttatcc tcctatattt ttaatattga
    48361 ataacttata aagtattata acattactta tgtttttaat attgaacaac ttataaaata
    48421 ttataacttt acttagattt tgggtcatat tcaaaatatt tatactttat agattgagaa
    48481 ataaaaaaaa tattttaatt aaaacagatt atgaggataa caaacttatt taagcagtat
    48541 gttttttgtt gataataata ttaaagtaga aataaaaata cttttttagt gaaaagctat
    48601 acaagaatac cgaaaaaaac ttataatata ataatgagat agatatttat aaatgaaaaa
    48661 aatttttctg gcaaagctta attaaaaagg taatagctag tgttgttatt taaattagca
    48721 tagaaaatca aataaaaaaa ttatgctaat gtatagacat tgataaggta gaatttaagc
    48781 cttttgaatg ctatattaaa ctagagtaga tattcttatg aatttcaaaa tagggcagga
    48841 ggaaaaagga aataaaacta taaaaaatat aaagtattta tataaattta tagagtttat
    48901 acgtcagaag atttcagtta ggagggcata aatggagctt tatgaagcca ttttttatag
    48961 aaaatctgta agaaattttt ccagtaagaa gataaaaaag ccattgatgg aagaaataaa
    49021 aagaagttgt aaaaatttag aatacttaaa tgaagattta aatattaaag cacatgttgt
    49081 tgatagaggt catataatac attttttgat gggtaaggaa tgtaaagtaa aggcacctca
    49141 ttatatagta gttacatcag acaaaggtga ggactattta cagaatatag gatttgctac
    49201 agaaggtgta gttttgcgcc ttacaacatt gggaatagct acttgctggt tggaaggaaa
    49261 cttaaagaga gaagatatct tggaatttgt agatattggg aataagaatt ttgaagaaga
    49321 tgaggaagat gatttagatt atctagctgg agtaaaaaca gaagaagatg atgaaaacaa
    49381 aatagaaaat ccttatataa taatagcatt tggatatcca gcaaaaaatg aagagttgtt
    49441 taggacgaga tatgctagac ctgatagaaa gccagtaaaa gatatgataa ataagataaa
    49501 taggaataga aagtggaaag atatcttaga attgacaaga agagctccat ctgtaaaaaa
    49561 ttctcaaccg tggtatttcc ataaggatga acgaggtctt catttatttg aaaagagacc
    49621 taagaaacac tgtgaagata tgaataaggt tagcttaggt gtagccttaa gacattttga
    49681 tatagcttgt ataaaaaata aaatagatgt ttcatatgaa aaacttccta taaggaataa
    49741 aataggaaaa tcatatttta taactgtagt cgagcatgtg aaaccagaag aagaaaccca
    49801 agaagaaaat gtaactctag aaaaagaaga atcacaagat gaataaaatt ttggaagggg
    49861 atattggata tgtataagtg tggaaaatgt aatcttagag gctgttctaa tggaaatatg
    49921 gataaattaa tgaagatatg tccaactaga gaaattgaac tacaacaaaa agcaaagcaa
    49981 ctttatgaag aagaagagaa tagaaaaata gcttataatg ctggattagt tgaagcagag
    50041 gggtattgtc aatatactag actaaaagag attatagagt ttgctaaaaa gtgtggttat
    50101 aaaaagattg gcatagcatt ttgtattgga ctaaaaagag aaatgcaaac agcacaaaaa
    50161 atatttgaac atcatgggtt tgaagtattt tctgttatat gtaaaaatgg ggcaatgcct
    50221 aagagtacaa ttggactaga agcatctcaa acaatatcaa agtgttcaga tgaggtaatg
    50281 tgcaatccaa taggacaagc tttattttta aatgaagcta aggttgacct taatgtaatt
    50341 cttggattat gtgttggtca cgatacttta gccatgaaat atatggaagc tcctataaca
    50401 actttagttg tcaaagatag agtaactggg cataatccag ttgctgcaat atataatgca
    50461 gaatcatatt ttgagaaaaa attgtttgat aaagatgaag aataattcaa agacttattg
    50521 acataaaaag caattagtgg tttaattata aaaaagataa aactttaaat tatcctatag
    50581 atacataaag ttatataaat aaagaaaaat attacagcta aattccttaa agtagtccag
    50641 agagactaaa gggttataga taatgttagt atataacctc tagtaaatct tctagaggtt
    50701 tttgatgagt ataaaataaa aatttaaata taataaagtc ttttaaacta gtccagagag
    50761 actgggaaag atatctcata gctaatgaat ggagttatgt atagataaaa tccttatgcc
    50821 tcttttggtg tgaggatttt tttatgatta tttaggcttt attaaaaagg agatgtaaaa
    50881 atgataaatg gtaaagaaaa attgatagtt gcaattgata ctgacgaatt tgataaagct
    50941 aaagaactaa tagacagatt agaagatagt gtagacatat ttaaggtagg tctagagcaa
    51001 tacgttgcca caaaaggtaa aacaatagat tatcttaaag aaaaagggaa aaaaatattt
    51061 ttagacctta aattccatga tataccaaat actatgaaaa gtgcagtgag agctgcagtg
    51121 agagataatg tttggttaat gacaatacat gtatcagata tggaaggaat gagacaatgt
    51181 gcactaattg ctagggaaga agcagaaaga ttaaatatag aaaagccatt agtagttgga
    51241 gtaacagttt taacttcatt aagtaaccaa gatttacagg acataggatg taatatgaca
    51301 acagaagagt tagctataaa gagagctaaa ctggcaaaag aatcaggcat agatggggtt
    51361 gtttgttcag cgcaagaagt agataaaata gtagaagcat gtggagctga ttttataaca
    51421 gtttgcccag gaataagacc taaatcagca gaggttggag accaaaaaag agttgtaact
    51481 ccatcagatg caataaaaaa aggtgctcat tatttagttg ttggaagacc tataacaaaa
    51541 gcagaaaatc caagagaatc agctattaat attgtaagag aaatagaaaa tgcttagata
    51601 aaatatttgg gaggtagctt atatgaaggg gaaattaatt ttagaagatg gaacagtatt
    51661 tgaaggaaaa gcatttggat atctaaagga agctattgga gaagttgtat ttaatacttc
    51721 aatggctggg tatggagaaa tattgacaga cgcaacttat tatggacaaa tagttactat
    51781 gacttatcca cttataggaa actatggaat aaatttagaa gatgttgaat caaataaggt
    51841 gcaagtaaaa ggacttattg taagagaaaa aagtgataac ccaaataatt ttagatgtga
    51901 aatggatata gacacatatc taaagcaaaa taaggtaata ggacttgaag gaatagatac
    51961 aagagcatta actaaaatta ttagaaacaa tggaactaag agaggaatta taactttaga
    52021 aaatgtatca ttacaagatg ttcagtctaa attagaagag cattgtaaca aagattcagt
    52081 aaaaaatgtt acaagaaaag aaattgaaaa tataaaaggt actggagcta aagttgtagt
    52141 tatagacttt ggtgttaaaa atagtgtact agagtctctt aaagcttgta actgtgatat
    52201 aactatagca ccagcaatga ctacagtaga agaaatttta aatataaatc ctgacttaat
    52261 cttattatct aatggaccag gaaatccaga ggatataaat gatatagttg aaaatataaa
    52321 aaaattaata ggcaaaaagc cattgacagg aataggttta ggacatcaaa ttttagcatt
    52381 ggcattagga ggaaagactt caaagcttgt atttggacat agaggaggta atcacccagt
    52441 taaatgttta gaaactggaa aggtatttat aacttctcaa aatcatggat ataatgtttc
    52501 agaaatgcca gaaaatacag aggtaactca cataaattta aatgacaata ctatagaagg
    52561 aatgagacat aaggaattac caatatatag tgttcagtac attccagaat acacaacaaa
    52621 agaagattta gattgtatat ttaataaatt cttagcatta atatagattt agtattattt
    52681 acagatagta aaaaccattt agataaggag aaaaatttat gcctaaatta gatagtataa
    52741 agaaaacttt agtattaggg tcaggaccaa taataatagg tcaagctgca gagtttgact
    52801 attcaggaac acaagcttgc cagtctttga aagaggaagg gattgaagtt gtattaataa
    52861 atagtaatcc agccactata atgactgata aagaagtggc agataaaata tatatagaac
    52921 cattaacaat agaatttata gaaaaaataa tagaaaaaga aagaccagac agtttacttg
    52981 caggtatggg tggacaaaca gggctaaacc tagcagttga actttatgaa aaaggcatac
    53041 tagataaata tggagtaaag ataataggta cttcagtaga atctatcaaa aaaggtgaag
    53101 atagagatat atttagagaa gtaatgaaag aaataaatca gcctgtaata gttagtgata
    53161 tagttacaga tttacaagca ggtcttgatt atgcacttac aattggttat ccagtagttg
    53221 taagaccagc atacacacta ggtggaactg gtggtggtat agctgataat gaggaagaat
    53281 taagagaaat actttcacat ggtctacaat taagtcctgt tggacaagta ttaattgaaa
    53341 agagtataaa aggttggaaa gaaatagaat atgaggtaat aagagattca aagggaaatt
    53401 gtatagttgt atgtaacatg gaaaatatag acccagtcgg agttcataca ggagacagta
    53461 tagttgttgc tccaactcaa actttaagta acaaagaatg tgcaatgctt aaaaaagcat
    53521 ctttagatat attaaatgca gtagaagttc aaggtggttg taacgttcaa tttgcactta
    53581 atccacatag ttttgaatat gcagttatag agataaatcc aagagttagt agatcatcag
    53641 ctttagcatc aaaagctaca ggatatccaa tagctaaagt ttcatcaaaa atagctttag
    53701 gttatacttt agatgagata gaaaatgcag taactaaaaa aacttatgct tgttttgaac
    53761 caactataga ttatgtggtg gcaaaaatac caaaatggcc ttttgataaa tttaaaaaag
    53821 caaatagaaa attaggaact aagatgatgg caactggtga aattatgagt ataggaagta
    53881 actttgaggc tgctattctt aaagggataa gatcacttga aacaggaaaa tattcattag
    53941 ttcacacacc atctgaggag agaagcattg aggaattaaa gaaaagagtt gtagtacctg
    54001 atgatgagag actatttgac ttagctgaaa tgataagaag aggctataaa gtagagatga
    54061 tagaacaaat tacaggtata gataaatggt ttataaataa atttaaatgg atagtagagc
    54121 aagaagaaaa attaaaagtt atgaaaattg aagatttaac taaagactat cttcttgaat
    54181 taaagaaaaa gggattctct gataaaggta tatctgactt aatgaagata agtccagaaa
    54241 aattatatga attgagaagt ctatacaata tcaaaccagc atataaaatg gttgatactt
    54301 gtgcaggtga gatagatgct atatctcctt actattattc aacttatgag caatatgatg
    54361 aggtagttgt tagtgataag aaaaaggtga tagtattagg ttctggtcca ataagaatag
    54421 gtcaaggtat agaatttgac tattgttcag ttcattgtgt aaaatcttta agaaaaatgg
    54481 acatagaaac tataatcata aacaataacc ctgaaactgt aagtacagac tttgatacat
    54541 cagataagct gtattttgaa ccattaacag aagaagaagt tttaagtata atagaaaaag
    54601 aaaagccaga aggtgtaata ctacagtttg gtgggcaaac agctataaaa ttggctaagt
    54661 ttttacatga aaaaaatata cctatactgg gaacagattt tgatgatata gatgcagcag
    54721 aagatagaga aaaatttgat gatttactag agagactaga tataaataga ccaaaaggaa
    54781 agggtgtatg gtcacttaac gaaggtattg aagttgctaa tgaattaggt tatccagtat
    54841 tagttagacc atcttatgta cttggtggtc aaggtatgga aataacttat aacgaagaaa
    54901 aattatctca atatctaca
//
LOCUS       DAMKPG010000016        54569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMKPG010000016 DAMKPG010000000
VERSION     DAMKPG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.018"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(409..3516)
                     /locus_tag="PC621_001032"
     CDS             complement(409..3516)
                     /locus_tag="PC621_001032"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF4108204.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3609..3841)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4016..4543)
                     /locus_tag="PC621_001033"
     CDS             complement(4016..4543)
                     /locus_tag="PC621_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF4108205.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4578..5363)
                     /locus_tag="PC621_001034"
     CDS             complement(4578..5363)
                     /locus_tag="PC621_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF4108206.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5373..5630)
                     /locus_tag="PC621_001035"
     CDS             complement(5373..5630)
                     /locus_tag="PC621_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF4108207.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5649..6107)
                     /locus_tag="PC621_001036"
     CDS             complement(5649..6107)
                     /locus_tag="PC621_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF4108208.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6147..6851)
                     /locus_tag="PC621_001037"
     CDS             complement(6147..6851)
                     /locus_tag="PC621_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF4108209.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6861..7496)
                     /locus_tag="PC621_001038"
     CDS             complement(6861..7496)
                     /locus_tag="PC621_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108210.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            7877..8917
                     /locus_tag="PC621_001039"
     CDS             7877..8917
                     /locus_tag="PC621_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4108211.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(9380..10735)
                     /locus_tag="PC621_001040"
     CDS             complement(9380..10735)
                     /locus_tag="PC621_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF4108212.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(10761..11030)
                     /locus_tag="PC621_001041"
     CDS             complement(10761..11030)
                     /locus_tag="PC621_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF4108213.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(11320..13260)
                     /locus_tag="PC621_001042"
     CDS             complement(11320..13260)
                     /locus_tag="PC621_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108214.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            complement(13444..14919)
                     /gene="spoIVA"
                     /locus_tag="PC621_001043"
     CDS             complement(13444..14919)
                     /gene="spoIVA"
                     /locus_tag="PC621_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF4108215.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(15091..16386)
                     /locus_tag="PC621_001044"
     CDS             complement(15091..16386)
                     /locus_tag="PC621_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4108216.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            complement(16570..17979)
                     /locus_tag="PC621_001045"
     CDS             complement(16570..17979)
                     /locus_tag="PC621_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4108217.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(18031..19170)
                     /locus_tag="PC621_001046"
     CDS             complement(18031..19170)
                     /locus_tag="PC621_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4108218.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            19489..20139
                     /locus_tag="PC621_001047"
     CDS             19489..20139
                     /locus_tag="PC621_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4108219.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYIIKHAK"
     gene            complement(20296..21315)
                     /locus_tag="PC621_001048"
     CDS             complement(20296..21315)
                     /locus_tag="PC621_001048"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF4108220.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(21346..21960)
                     /gene="plsY"
                     /locus_tag="PC621_001049"
     CDS             complement(21346..21960)
                     /gene="plsY"
                     /locus_tag="PC621_001049"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF4108221.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(21995..23314)
                     /gene="der"
                     /locus_tag="PC621_001050"
     CDS             complement(21995..23314)
                     /gene="der"
                     /locus_tag="PC621_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF4108222.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(23348..24676)
                     /locus_tag="PC621_001051"
     CDS             complement(23348..24676)
                     /locus_tag="PC621_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF4108223.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(24914..25114)
                     /locus_tag="PC621_001052"
     CDS             complement(24914..25114)
                     /locus_tag="PC621_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108224.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(25116..26048)
                     /locus_tag="PC621_001053"
     CDS             complement(25116..26048)
                     /locus_tag="PC621_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF4108225.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(26093..27025)
                     /locus_tag="PC621_001054"
     CDS             complement(26093..27025)
                     /locus_tag="PC621_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF4108226.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(27080..28369)
                     /locus_tag="PC621_001055"
     CDS             complement(27080..28369)
                     /locus_tag="PC621_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108227.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(28398..29090)
                     /locus_tag="PC621_001056"
     CDS             complement(28398..29090)
                     /locus_tag="PC621_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108228.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(29105..29866)
                     /gene="pgeF"
                     /locus_tag="PC621_001057"
     CDS             complement(29105..29866)
                     /gene="pgeF"
                     /locus_tag="PC621_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF4108229.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(29863..30324)
                     /gene="nrdR"
                     /locus_tag="PC621_001058"
     CDS             complement(29863..30324)
                     /gene="nrdR"
                     /locus_tag="PC621_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF4108230.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(30346..30609)
                     /locus_tag="PC621_001059"
     CDS             complement(30346..30609)
                     /locus_tag="PC621_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF4108231.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(30713..31486)
                     /gene="sigG"
                     /locus_tag="PC621_001060"
     CDS             complement(30713..31486)
                     /gene="sigG"
                     /locus_tag="PC621_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF4108232.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(31578..32300)
                     /gene="sigE"
                     /locus_tag="PC621_001061"
     CDS             complement(31578..32300)
                     /gene="sigE"
                     /locus_tag="PC621_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF4108233.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(32325..33197)
                     /locus_tag="PC621_001062"
     CDS             complement(32325..33197)
                     /locus_tag="PC621_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF4108234.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(33488..34762)
                     /locus_tag="PC621_001063"
     CDS             complement(33488..34762)
                     /locus_tag="PC621_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF4108235.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(35046..36203)
                     /gene="ftsZ"
                     /locus_tag="PC621_001064"
     CDS             complement(35046..36203)
                     /gene="ftsZ"
                     /locus_tag="PC621_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF4108236.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(36393..36746)
                     /locus_tag="PC621_001065"
     CDS             complement(36393..36746)
                     /locus_tag="PC621_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF4108237.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(36845..37519)
                     /locus_tag="PC621_001066"
     CDS             complement(36845..37519)
                     /locus_tag="PC621_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF4108238.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(37524..38222)
                     /locus_tag="PC621_001067"
     CDS             complement(37524..38222)
                     /locus_tag="PC621_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF4108239.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(38245..38985)
                     /locus_tag="PC621_001068"
     CDS             complement(38245..38985)
                     /locus_tag="PC621_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF4108240.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(39255..40481)
                     /gene="murG"
                     /locus_tag="PC621_001069"
     CDS             complement(39255..40481)
                     /gene="murG"
                     /locus_tag="PC621_001069"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF4108241.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(40499..41629)
                     /gene="spoVE"
                     /locus_tag="PC621_001070"
     CDS             complement(40499..41629)
                     /gene="spoVE"
                     /locus_tag="PC621_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF4108242.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(41681..43036)
                     /locus_tag="PC621_001071"
     CDS             complement(41681..43036)
                     /locus_tag="PC621_001071"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF4108243.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(43055..44023)
                     /locus_tag="PC621_001072"
     CDS             complement(43055..44023)
                     /locus_tag="PC621_001072"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF4108244.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(44064..45437)
                     /locus_tag="PC621_001073"
     CDS             complement(44064..45437)
                     /locus_tag="PC621_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF4108245.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(45604..47583)
                     /locus_tag="PC621_001074"
     CDS             complement(45604..47583)
                     /locus_tag="PC621_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF4108246.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(47595..47942)
                     /locus_tag="PC621_001075"
     CDS             complement(47595..47942)
                     /locus_tag="PC621_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108247.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(48428..49363)
                     /gene="rsmH"
                     /locus_tag="PC621_001076"
     CDS             complement(48428..49363)
                     /gene="rsmH"
                     /locus_tag="PC621_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF4108248.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(49393..50139)
                     /locus_tag="PC621_001077"
     CDS             complement(49393..50139)
                     /locus_tag="PC621_001077"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF4108249.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            complement(50536..51822)
                     /locus_tag="PC621_001078"
     CDS             complement(50536..51822)
                     /locus_tag="PC621_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF4108250.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(51837..53102)
                     /locus_tag="PC621_001079"
     CDS             complement(51837..53102)
                     /locus_tag="PC621_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF4108251.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            53418..54515
                     /gene="ychF"
                     /locus_tag="PC621_001080"
     CDS             53418..54515
                     /gene="ychF"
                     /locus_tag="PC621_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF4108252.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        16924 a         9992 c         5790 g        21863 t
ORIGIN      
        1 acatataggt ttccctatat gctgactatt ttagctacta actcatgact aaattcacga
       61 gtgtgcgtag ctatttttta atcaatactt aactttttgt tttcaccata catcaaaacg
      121 cacccctttg cataataaat aaaccaccct gcagaacaca cgataaattt ataaataaat
      181 ttatcagaag tagcttacta aaaatattat tcatcctcac ttaaacgttt attcattaat
      241 attctatcac atgacataaa taatttatat gatttatata gatttattta ttgtaaattt
      301 tggaatatta aaacatttta ttgaatagat aattatatta cataaaaaat ccctatagaa
      361 atctataaac taatttctat agggattttg gttaactaac tattcttttt atttttttat
      421 aacttttata cctgtcatat ggtcatttaa gttttgttcc tctaaagatt tatcatctac
      481 tctgtttatt tctaaagcta aagtttcaga cttgatatat tcattaaaat ggtcaacagc
      541 cttagcaatt tcatcatcac cacagtattc tattactata ttatctaata cttcaaagtt
      601 attagatttt cttaattgct gtactttaga tataaattct ctagcataac cttcattaat
      661 caactcttca gttagcttag tatctagtat aacaaaaaga ttgttttcaa cagatacatt
      721 aaatccttcc ttagcagtta tacctattaa tactaaatcc ttattaaatt caaaagcttc
      781 gccatctaaa tcaactgtta aagtctcacc tttttcaagt ttattagcag tttcatgagc
      841 atttaattta cttaatgctc caacgaataa attcatctta gaacctaata atggccctgc
      901 ttccttaaag tttggtttta aactaaagtt catatactca tctaaatcct tagcaaatat
      961 tacctcttta acattaagct cttcttttat taaatcaact acatcactta ttgtatcttc
     1021 aaacttacca tctactaaca ctttttgtaa tggttgacga acttttattc tttctacttc
     1081 tcttgaagct ctacctaaag taactaagtt tttagctaaa tccatttttt cttctaattt
     1141 agtatctact aactcttcat tacatttagg atattcagct aaatgaacag atacttctcc
     1201 tgttaagttt ctatacattt cttctgacat atatggtgca aatggagcta tagctctaca
     1261 aagaccatgt aacaattcat aagttgtatt gtacactgct tttttatctt ctgttaattc
     1321 agttgcccag aatcttcttc ttgaacgtct tatataccag ttagataaat catcatttat
     1381 aaaatcttgt atcatcctta aagttttatt taactcaaat atagctaaat tttcttctac
     1441 atctttcatt aaattattga acttagataa tatccatcta tcaagttctg gtctatcttt
     1501 atattctaca aaaaattcag ttggatttat atcatctgtg tttgcatata aagtaaagaa
     1561 gttatataca ttcttcatag tacctataaa tttactttgg atttctttta atccatccat
     1621 atcaaattta gttggagtcc atggtggaga aacatatagt aaataccatc ttaatgcatc
     1681 tgctccatat tggtcaaaca attccattgg atttactgta tttcctcttg atttactcat
     1741 tttcttacca tctttatcaa gtacaaggtc tggaactaaa acactcttat atggtgcttt
     1801 tcctaataca aatgttgata ctgcaagtaa tgagtagaac caacctctag tctggtctat
     1861 accttctgat atatagtcag ctgggaacaa ttggtcaaaa ttttccttat tttcaaatgg
     1921 atagtggtgt tgagcaaatg gcatagcccc actatcaaac caacagtcta taacctctgt
     1981 tactcttgtc attggttttc cacatttttc acattttaag tgtatatcat ctacatatgg
     2041 tctatgaagt tcaactgttt ctggatttac atcttctata gctttttcag ctaattcagc
     2101 tcttgaacca actgaatctg tatgaccaca ttcacatctc catataggaa gtggagttcc
     2161 ccaatatcta gttcttgata ttgcccagtc atttagattt tctaaccagt ttccaaatct
     2221 cttctctcct acaaaacttg ggaaccattt tacaccatta ttattttcta ttaatttatc
     2281 tttaagttta gtcatctcta tataccagct tggttttgca tagtatacta atggagtttg
     2341 acatctccag cagtgtgggt aattatgaac tactttttct ttgctatata atttattttc
     2401 agaagctagc cattttatta tttcaacatc tagcccatct tccattacaa atctaccttc
     2461 ccaaggagta gatgtaaatt taccagtttc atctactggt tgaataactg gtaaatcata
     2521 tcttttacct gtgttgtagt catcttcacc aaatgctggt gctgtatgaa ctataccagt
     2581 tccatcttca gtagttacat aatctcctac tgtaataaaa aatgcttttt tgtcaacttc
     2641 tacaaaaggc attaattgtt catattctaa accttctagg tctttacctt tcatttcttc
     2701 taaaacttca taatcttcac ctagtacttt atcagctaaa ggtttagata cataatatac
     2761 ctcatcacct tttttaactt ttaaataatc tacatcagca tttactgtta atgaaacatt
     2821 tgatggtaaa gtccatggag ttgttgtcca tgctaagaag tattcatcag catctttttt
     2881 cttaaattta gctataactg tattagtttt tacttcttta tatccttgtg caacctcatg
     2941 actagcaaga ccagttccac atcttgggca ataaggaagt attttatgcc cttcatatat
     3001 atatccttct ttgttgaatt tgtttagtat ccaccataca gactctatat agttattatc
     3061 taaagttata tatggattat ctaaatcaac ttcataagcc attctctcag tcattattct
     3121 ccattgtttt tcaaatgaga aaactgactc tctacatttt tggttaaatt cagctattcc
     3181 atattcttct atttgttgct tgctagtaag acctaactct ttctctactt gtatttcaac
     3241 tggtaatcca tgtgtatccc aaccagcttt tctctttact tgataaccag acattgtttt
     3301 atatctacaa actgaatctt taagcgttct agataaaaca tggtgtactc caggatttcc
     3361 attagctgtt ggaggtcctt catagaatac gaaacttggg tcatctttcc ctttttctaa
     3421 agttttttca agtatattta tgtctttcca ataatcaaaa acttgtgctt ctgcttgttt
     3481 tacagaactg tctaccaatg gcttaaattt cgccatgtaa atcacccttt cctactaaat
     3541 attataattc caaaattttt tattctttca cataaattta aatactaaaa aactcgcccc
     3601 ctttaaacca aagggacgag ttataaatcg cgttaccacc ctaattctat atgtaaacta
     3661 aatactagct acatataaca ctctaatagt ataacggtac taaccggcac agcttagtag
     3721 aatttaatcg ttcagcctgc agctcaagag tgatttttat ctttataaat tcattggttc
     3781 tcagcatacc caattctctg taaaattatc taaagataaa tgtctctatc aacgctttta
     3841 aaaatttatc tgtgtttata tgaaatttta taacaaataa tttgaatagt caatatattt
     3901 ttgaaaaata taataatttc tatttttctt acatttttat cctattttta atctttgtta
     3961 tagatttctc aaactcacac agttattcta cataaaaaat aaacaaatta caagcttatt
     4021 ctaaagttgt agcagcttca tcattactat aattatacac tgttgttcct tcaaatgcat
     4081 ttacacattt ttcatctaca tcataaaaaa tctcatcaac acttcttatt tcatcttcta
     4141 acagtgattt aaatttattt ctaaatatct taaattcttt gactaaagca tcatactctt
     4201 ttcttatctc tatgacacgg ttattagctt gttctataat ttgtctagct tttaaatcag
     4261 cttcttcaac aattatctta gcctttttat tagccgcgct acaagtgtct tctgctgcac
     4321 tttgagcagt tattaaagta gcttttagtg tttcttcaat attttcatac ttgtttactt
     4381 ggtcttgata taaccctaat ttttctttaa gagctgtatt ttccctacat agagattcat
     4441 aatcttcctt gatgatatct aaaaattcat caacctcttc atctctatac cctcttaaac
     4501 cttttttaaa ctctttattt tctatctcaa ttggagttag cattacattt cctcccagct
     4561 ataatttaaa ttagtcctta tacaattaat cttgcttgaa cttttatttt tccttttttg
     4621 gtaatatctc ctatttgtgt taccttagct cttcctttgc ctctaataga aatcaaatca
     4681 tcatttttta ctattttaga agttgaagtt atcttttcat aattgacatg gactttttct
     4741 ccatttatgt attttgcact atcttgtcta gatatattgt aaataccact tatcacacaa
     4801 tctaacctat ctgaagatac tgtaaatgat acttctttat attttgaaga actattaaca
     4861 atattttctc tggaaatttc ctttacaatt acattatttc ttgaaacctt ttctaagttt
     4921 acaattataa aatcgcaaat atcactactt acaactactt gacaaaagct atcatgtata
     4981 attatatctc caattttttc tctttttata cctagaccca gtattgaccc taagtaatct
     5041 ttatgagata tatttttaaa cttgaaattc ccttctattt gtaaaaatct aagagtatct
     5101 tctatatctt catattccat gtaaaatgga taaataaata cagtgcttct ttcagcttgt
     5161 tcataacctc catccacact gtattttatt tcatttgtag aatttaatat tgctacagca
     5221 tttttcactt catatggatt aagaaactcc gtagacttaa catcatagtt ttttatacaa
     5281 ctattagcct tgtctatcac tttaaacatt ttatttttca agtctatatc ctttatgtga
     5341 tttgttagtt ttaatttatc catcctatca ccttagaata taaccaatac taattgtctt
     5401 aggaacatca ataatactat tgcaattaaa ggagaaaaat ctataggtcc atttgtatat
     5461 ttatacatta tagaccttat tggcccttct attggctctg taagattttc caaaatatta
     5521 aaaattttac tttgtcttcc atttggaaac caagtcatca agctttttat aactatcatc
     5581 catgctatta tatctaaaag ataataaagt gcaattctta ttgttcccat atattaaccg
     5641 cctttttact atttattttg ccaagggaag aatgctttac tttctaattc ttttttcata
     5701 tttgcatcta tatccacatt attaggagct agtataaata ttgatttaga aaccttttgt
     5761 atacttccat ctaatacata aactgcccca ttcataaagt taaatataga tttacgaact
     5821 tcagaattag tcaatccttc caaattcact ataactgccc ttctttgttt tagatggtct
     5881 gctatagttg cagcttcatc ataaacctta ggttcaacta ttacaacttt catttggcta
     5941 gttgtatgta aatttacaat tttatttgct tttgcagtac taaagccact gttcatttct
     6001 tcttcttgaa ctatatcgtc catgccactt tcatattcat cttcaatata atcttcttct
     6061 tcatcaacta tccaattctt aaatttagat attattccat ttgacattat taacttcctc
     6121 cttaaaattt attcaaattt gagtaattat tttttattgt agtttctttc tccaaagatt
     6181 gatgttccaa ctctaacaat ggtagcccct tcctctatag caatttcata atcgtgactc
     6241 attcccattg acaaaacatc catccctaca ttagggatgt ttagttcttt tatttgtttt
     6301 gataaatctt taagaccttt aaatacacct cttatctcat cttcatcttc tataaaaggt
     6361 gccattgtca ttagaccctt tatttttata tttttatatt tttctgacac ttctttgaca
     6421 aaatctacaa cttcttctct atcaagtcca tgcttagact cttctttaga tatattcact
     6481 tgtactaagc aatctatttc tctgtttatt tgttctgcac gtttttgaat ttcttcgcat
     6541 aatgcaattc tatctaaaga gtgtatcata caaactttat cgattatgta cttaacttta
     6601 ttggtttgca aagttcctat caggtgccag tttaatttgt cacctataat atcatacttt
     6661 ctagctaatt cttggggttt attttcacca acagaagtga ttccttccct cattgcctca
     6721 ttaactaagt ctacatcaac agtctttgtg actgccaaaa gggtaatctc tttgctgtct
     6781 ctatctactt tttttgcagc tttatcaatt ttgtttctta tctcagacaa attatcttta
     6841 atagaaccca ttattatcac ctactttatc cttatatttg tgtttatact gttaggtctt
     6901 aaaattattt tgtcatatag attaacactt gtaactccat ttatgtcatt tttataataa
     6961 tccacaaata cagaagattc attctcataa gttattcctt ctaatatgac aaattttaca
     7021 ctttgtgatt gttcatcaat tacatataca ccacgtttat tgtcttttgt ttttatagaa
     7081 gttttaggta tttttaaaca ttccatctga ttatatatta tatcaaattc ttcgacactt
     7141 gtatcctgat ttgcaacaat ttggttactt atttttaatt ttaccattat tttattatta
     7201 ttttcatata ttttgtaaac ttttgcatct aatttttgct catgatattc aattattaca
     7261 ttttgatttt tttcaaagtt attagaatct tctttactga tgtatgtaga tagataatta
     7321 ctttggtcat tggcatcttt ttctatagtt ccttttacag gaatctttgc atcaactttt
     7381 tcatttttta acaccaacgt atctacattt ttgctaatta tcattactaa tatattaaat
     7441 actaaatata taagaatacc aagtatcact aatctggtat attttacctt tttcaaagac
     7501 tctcccccta taaaattact ttcattataa gtacttcttc taaaaaatgt ttattccttt
     7561 tttataagct aatattttac gttctaataa ttattttttc catattaaat ttatttttta
     7621 tcaatcttcc tatacaagac tgcattattt atatataatt gtattataaa gtataagttt
     7681 aatttacatt ttatacatta agcttacttt atatgttttt atagttattt ttatttttag
     7741 atgcaatata aaaccaatta agatttaaaa taacttttgt tatgttgaca tttatttagg
     7801 taatcattta cgaatcagga taacgttatc ataacaaaag tttatgtcct aaaaaaaata
     7861 caaggaggat tttcttatgt cagaaatttt attaggaaca ggtttacttt tattggttct
     7921 atgttgtttc acaattttta gttacaaggc cccatatggt atgaaggcta tgggagctct
     7981 agcaaacgct gcatgtgcta ctttcttagt agaggcattc aacctttctt tacttggtga
     8041 tgttttaggt atggaatttt ttagaagtgt tggagctgct aatggtagtc ttggtggagt
     8101 tgctgctgct atacttgtgc ctttagcctt aggtgtatct ccagtttatg cagttttaat
     8161 aggattatct tgtttagatt tttctatatt atctggattc gttgctggat actttgtttc
     8221 tttcttaatt aagtttatag agaaaaaagt gccacctgga gtggatttag tggttgtaat
     8281 tcttctatgt gcaccgctat ctcgtcttat agcatcaaat gtagacccat tagttaatag
     8341 tacactacta caaattggac aagttttgat agaagctacc aacattagcc ctataataat
     8401 gggaattata ctaggaggca taataacagt agttggaacg tctccactta gttcaatggc
     8461 tcttacatct ataattggtc ttactggtct tcctatggca ataggagcat taggtgcttt
     8521 tggttcagct tttatgaact caacactatt tgcaaaattg aaattaggtt ctaaaaaaga
     8581 tattatatct gttgctattg aaccattaac ccaatcagac ataatttcag ctaatccaat
     8641 acctatatat tctactaatt tctttggagg agccttttct ggtatcattg tagccttgat
     8701 gggtcttgta aacatgacac caggtacagc tacacctgtg gctggttttg ctataatgtt
     8761 tgcttataat ccagttcaac aagttcttat aacagctggt ttatgtgctt tattcaatgt
     8821 aattgcagga tttttaggtt caaaaatatt cagtaccttt aaagttctta ctgctggtga
     8881 aatacgtggt actgatgtag aagaagaaat tgcttaaaaa acaataagat ttataaattt
     8941 atatctcaag tagtagagtt ttatattgta ggcaatttta ttaattgcaa acgttttatt
     9001 ttttatttat gtttatgatt attatgtgtg ttgaatattt agataaattt ttatttatta
     9061 tttttattta gatttttgtt tagtttttta tttggttgat atattaggaa taaaaattag
     9121 tatagtttat tattagttag atagcataac tttttagata cataaaaaat tttatattaa
     9181 ttagatatta ttaaaataaa agcgatacat gaatttttaa atgtccatgt atcgctttat
     9241 ttttgccata atcttaacaa ctgattttta aagtttatta tcttaacgaa gttcattatc
     9301 ttatataata tttaaatgca tagacttctt taatttaacc atctaaatat ttaattaaat
     9361 attattatat gtaatataac taatttttaa atgaatgtat aggagctgga atccttcctc
     9421 ctctttgagt aaataattca gatgaatttg tacttatagg cattactggt gctcgtccta
     9481 aaagtcctcc aaattctacc atatcaccta catcacatcc tggggctggt ataattctaa
     9541 cggctgtagt cttattatta atcataccta ttgctgcctc atcagctatc atagctccaa
     9601 gcgtacctgc tgtagtattt cctggcacag ctatcatatc aagacctaca gaacaaatag
     9661 cagtcattgc ttcaagctta tctatactta aagcaccttt aataacagca tcaatcatac
     9721 ctgcatcttc agatactggt ataaacgctc cactaagtcc tccaacatgt gaacatgcca
     9781 ttactccacc ttttttaaca gcatcattta aaagtgctaa tgctgctgta gtcccatgag
     9841 taccaacaac ttcaagaccc atttcctcta atatatttgc tactgaatca ccaatagcag
     9901 gtgtaggagc tagtgataag tctattatcc caaatggaac atccaatcta ctagaagctt
     9961 cctttgccac aagttgtcca actcttgtaa ttttaaatgc agtcttttta actgtttctg
    10021 aaacaacatc aaaaggctct ccctttactt tctctaaagc tctcttaaca acaccagggc
    10081 cacttacacc aacatttata attctatcag cctcaccaac accatggaaa gctcctgcca
    10141 taaatggatt atcttctact gcattacaaa atactactaa ttttgcacaa ccaaaacctt
    10201 ttgcatcttt tgttaattct gcagtttctt ttatgatttc tcccatttct ttcactgcat
    10261 ccatatttat accacatctt gtagaaccta catttactga agcacatact ttattagtac
    10321 ttgctagagc ttttggaatt gacttaatta gtatcttatc accttttgta tatccctttt
    10381 gaactaatgc tgagaatcca ccaataaaat ctatacctaa atcctctgca gccttatcca
    10441 atgtctgtgc aaactttaca tagtcatttt catctgttgc acctgcaata atggaaattg
    10501 gagttactga taccctttta tttacaattg ggataccaaa ttcacgctct atatcttttc
    10561 caaccttcac caaatcttta gcagaactca taatcttatt gtatattttt tctctagctt
    10621 tatctccatc agggtctata caatctaata aagaaatccc catagttata gttcttatat
    10681 ccaactgttc ttcatctatc atctttattg tttcaagtat atttcctaca ttcactatta
    10741 tcacctcccc ttactgtcta ctacagatta tgcattgaat taaatatttc ttctcgctga
    10801 atttttacat ccactgataa tttcttacct ctttcaacca agtcctcaac cgttttctca
    10861 aaagaattta atgatttctc taaatcaact atcataatca tagtaaaata atcctgtaat
    10921 attttttgtg atatatctac tatgttgata ttttgcttgt ataattcatc actaattcct
    10981 gccactatac ctggtttatc ctttccaatt actgttaaaa ttgctttcat aataatatcc
    11041 tcccttattt ttttataaat cttatatttt tacaaattga actatgtatt aacatatatt
    11101 tcatttattt gttagtaaac cactttttat gtatatattt aatatatttt aatacctata
    11161 cattcatttg tcaatttttt ttcaatattc cattattttt atactattta gaaaattcat
    11221 attttatata tgttttctca acaataaata cagttttcat aataatgaaa ggtattactt
    11281 agcaatctcg tgattttcta aataatacct ctctaaaaat tattttttta attttatgaa
    11341 tttaattata ctttttctag tatcaggatt tacaattaat atagaaccaa tcataattgg
    11401 tgttagtata caaaataaaa aaataatatc tttttttaga ttatattctt tagattctaa
    11461 tttataatta ttatttatta ttccatcata atcacttata tttgacttat ttttatttgt
    11521 atcttgattt ttgttagaaa tactttccct ataaggaaaa gaatgttcat tggtaccaaa
    11581 tactgatttt gagcttaaat cataattatc atcattttct gtaattggat ttttattgta
    11641 aaatcctttt tctttatcag aattattttc tttattaccg tctttaggta tatcatgagg
    11701 atttttattt tcattgctat cagtacttga ttctacatca tctttaggag tatttggttg
    11761 tttctcatca gaattattat tttcattatt attgttttga ttgttattac tatcattatt
    11821 ttcatttcca ttattcccat cttgattgtt atcatcctca tctctatccg ggtcgtctct
    11881 acctcctcct ccacggtcac catcaatatc atcattttcc cgttcatcat ctggcgtctc
    11941 ttggttgtct ccgcctccca ttataaatcc tggtttaaaa actatatttg aatacccttc
    12001 ttttaatcct cctaccactt ttactctaaa acaacgcaac ttaaaatctt caaaaaaaag
    12061 tatagcttga tctctaatat cctcctgttc ttcagatatc caattgattc catcgcttga
    12121 atattcaaca aaaaccttac ttgcactata cggcatatca taaaataaaa cagctacata
    12181 tccatttttt gtattttgaa attccagttc caaattgtct attgtttttt tctctataac
    12241 agatacattc aaaataggaa ctacatcatc atttattata attttttctt tatttaacat
    12301 ttctgtttta aactttcctg ttatatcaaa attttctccc ttttccaact tttcataata
    12361 atcagtaaaa tcccattcta catctatatc aagttgatta ccactagatg tttctaatac
    12421 catatatttg ggaaatttta tattttctat tttatcatct ttacttactt ttatttcatc
    12481 actaaataca ccaattcccc tatgacaaca tttcacaata ttatagccag aatctttagc
    12541 tatagagggt ataagctcat tataatcatc aattatataa actttcttac tttctgatat
    12601 aattgattgt gccaacttat tattatctct tagtaaccca ctttcattgt tgctactata
    12661 tgcttttatt gaagtttctt ctatctcaac atcaccttta gaataaacac ctatagcatg
    12721 ttctccattc acttcaatat ccttatttag tattctaata tcattttcag tataaattcc
    12781 tattgcatct tttccatttg cctttatttt ttcaacagat tcgtaggatg atttaatatg
    12841 taagtctcct ccttcttcaa cgtataaggc tgtcccatca gtagcaatta tgttgtttat
    12901 accaatactt atacttccac cattttctat atgtattact ccttcttttc ctccttcacc
    12961 tattatattc aattcattat catcaataat aaatctccca tgatttttta tcaatatttt
    13021 atatttgttg gtatctattg taaaattact gtcataagga atataaaatc taaactcttc
    13081 atctattacc atattacaag aaagagtata aactatttct tctttattat ttttattttc
    13141 ttgaacccac ctttcaaaat cttcaagaca attttcctct tgtccaaaaa ctttatgtgg
    13201 aaaacttata aaacacaaaa ctattatact cataaaaata aatatctcat actttttcat
    13261 cttacactcc tagaaatttt ttagctttga aacaatcctg tcgaaacata tacttcaccc
    13321 actataattt ttccacaatt atcaattatt gtcaatttat ataagtatct ttattaattg
    13381 ttataaataa aaagctactt actaaagtaa acagacgttt cactttaata agtagctata
    13441 tttttataac aaaatagtta taatattaga acttccttca tttacaattt tttgtaaagt
    13501 cttttgcatt ttaactctta tctcttctgg catagtatat aatttacttt gtaattgctc
    13561 ttttactaag tcatgtagtg acttaccaaa catatttgat tcccataaat ctgctggttg
    13621 ctcttcaaat acttccataa gatattttat catctcttca ccttgatttt gatttccaac
    13681 tattggagag acctctgtag atatatctgc ttttataata tgaagtgatg gtgcattggc
    13741 ttttaacttg ataccatatt gttttccttg tttcattatt tctggttctt ccagactaag
    13801 ttcttctaaa gaaggagcta ctactccata tccttttatc ttggcatcta ttaaagcact
    13861 ttctatttta tcatactcat ttttaacttt agatagtcta gttatcagac taagcagttg
    13921 ataatctcct tcaattttaa atccactttt ttcttcaagg acattataga ataaatcttg
    13981 ctttgtctgt aaatcaatat ttataacacc ttctcctagc tctacattat caacacctgt
    14041 atcttccaaa aactctaact cagaaaatcc ttgtataata ccttctatat ctcttatttt
    14101 acctatatct ataatacttt gttttaatgt tgttattatg ctacttttta tccaatgatt
    14161 tctttcaaga ccttcaaccc attttggtaa gttgattctt atttctgtaa gtggaaaatc
    14221 atataataca gcttccataa cctcctctat atcttcttct tccatttcaa ccacattcat
    14281 tggaagaact ggaacttcat acttttcttc caattcactt ctaagcatac tcgtttcctc
    14341 actttttgga cttaacgtat ttaaaaccac tgcaaatggt tttttaagat ttttcaattc
    14401 ttgtataact ctctcttctg gttctacata gctttttctg tcaattccag taactgaacc
    14461 atctgtcaag acaactatcc ctattgtaga atggtctttt ataacttttt tagttcctat
    14521 ctctgctgcc ttttcaaatg tcatagcttc ttgtgaccaa ggagtggata ctaatctttg
    14581 ttttccacct tcctcatgcc ctaaagctcc ttcaactatg tatccaacac aatctaccat
    14641 tcttactttt aaagatactg tgtcttttat ttttatttct acaccatctg ctggtacaaa
    14701 ttttggttct actgtcatta tcgttttacc tgaaccactt tggggtatct catctcttgt
    14761 tctatccttt ttaaattcat tgtctatatt aggtataacc aacttttcca taaattttct
    14821 tataaaagtt gattttcctg ttcttacagg tccaactact ccaatatata tatccccctg
    14881 agtccttttg gatatatctt cgtatatgtt attattcata ttattcctcc tatacatcta
    14941 atcttattaa attatattta gagttaaggg ttaatattct taaaatagaa aaataaagac
    15001 cagcaatatt aatttaattt acatatagaa atttgtaaat ttcaatgtaa attaaattaa
    15061 tattgctggt ccaaatagta gtgtattata ttaactgact ttagtagtat tttcatttga
    15121 cttattattc atctcaataa gttctttacg tctttctttt gtcatgttca tccatataaa
    15181 tgtaaacacc aatccaatta tggctactat agtaagaact atgaagtagt acctatatcc
    15241 agttacaccc ggaaatgcat ccaatatctt ccctgctgct aaaggacaaa gtacttctgg
    15301 aagatatcct aatgtagcaa ctattcctat agcagttcct gatattgata atgaataatt
    15361 accttcttca agtaacgaat agtgtaaacc ttgaattgca tacatagata catatattgc
    15421 aacacatcca atcaatagta tataaatcat agatgatttt gttggtgtaa atattacacc
    15481 taaaagacct gcaatcataa gtataaatcc ataagatata atctttgatg aacctacttt
    15541 atctccaagt aatccagaag cagctgatgc tactggtcta caatactgag ctaatattga
    15601 gattgcagat gcaaacacca cagtagctcc aaatgcttca gttgaataag gcgtaaaata
    15661 ataaaatgac atattagctg aatatgaaca aaacattatt attgttatta tccatgtatg
    15721 aggcattttt attactgcaa aaaaggcttc tttatcaaat tttgctgata cctctttttc
    15781 attatccttt aatttgaaaa atactaatac tccaactatt acatttattg ccgagtataa
    15841 tactataata gctgtaagac cagatttatc acttactttt gatgaaatat acccaaatag
    15901 tatcaatact agagatagat gaattgcatt ggtaattcca cgaccacttt ccataaatcc
    15961 aaaagctttt ccttgttcat tttcagatgc taacattcta acagctttta caagagctgg
    16021 ccaaaaagta agtattgttg ttattcccca taaggcatgg attaaaaaca caacaatata
    16081 tggaggataa agtgttaata caagaccaga tattcctgta ataattaaag aacctgaaag
    16141 caattttctg gctgaaatac ggtctgctaa aaaaccacca aaaaggtatg ataccattgc
    16201 aagtagacca taaatgcttc ctaagcttcc catttgtgta tttgtaagat taaatgcttg
    16261 tgcaaaagta tcataataat aattctttaa atatggtaaa gcatatataa atgctcctgc
    16321 tacaaagagt attatcaaga ctttgaaatt tttccaaatg cttggtttat ttacttcaac
    16381 tgacatactt cctccttatg taaatacctg taaattttca tgataattaa ttattgctta
    16441 ttttattgtt tattcaatat ttcggaatat ttattaatat attaggttaa tctatcaata
    16501 atttatggtg agttatgagc taaaagtaaa ctcttttagc tcaccataat aattatattt
    16561 ttatttatac taacgtccag agttattaga tatctttttt tcatagttct tccatagcat
    16621 taaacctgtt attataacaa aaagacctcc tcctatcatt tcaatctcat agattatatt
    16681 atcttttaaa gtctccataa ctggtgccgt tgagaaaagt atgccaacta cagttactac
    16741 aagtaccatt ctggcaatat tgatacaaac tgaatcactt tttgcaattg aatatggtct
    16801 ttcttcattt ggtcttgtct ttctaagtct aatataagat gtaaaaagca agacatatgg
    16861 aaataatgct gttaatgctg tcatagtaac aagaacattg tatatattat ctactgatgg
    16921 taataagttt gttcctacaa gaagtagact cactattata gcctgaacta ttactgcatt
    16981 tgatggtata ttgtatttat ttgtaacagt caattgctta ggaaatacac cttctcttac
    17041 atttccaaaa agcatcttta ctggacctgc tatataaagt attaatgctc ctagtactga
    17101 taaagttatt ccaaatgcta ctattcttat aaaccaactt cctataccta aatcttgtgc
    17161 aactttagct aatgcatcta gtatccctgt agacgctgca attttatctg gaggaagtat
    17221 cattgttatt gctactgaac ctaagatata gattcctcct attaaaacag ccacagttaa
    17281 tatagcttta ggaaagtttt tcttagcatt atccatttcg gttatgaaat tagcagtcgt
    17341 ttcagctcct gataatgcaa atattatagc agatatagaa actaatgaat tggcatctat
    17401 ttttggtatt atattttgga ttgtatatat cgatgctgat ggagcttttt tgagaataac
    17461 tacagacata aatgccatca ctataagtaa tatagcagga attgtagaac ctaatgctcc
    17521 tgtattcgta aaaaattttc caaatgccat cccacgagta cttactaagg acaacagcca
    17581 aaatattatg agtgaaagtg ctaaaataaa catcttattg tttgataaat ctggatttcc
    17641 aagcatatat gcaatattta tagctaaaaa tgtcaagaaa gaagaatacc aaaatatctt
    17701 tgatgtccaa tttagccatg acaccataaa tccccacttc tctccatagg cttgtttaac
    17761 ccattctcca agtccaccat cttttggata tgttgcagcc aactctgcac atataagtga
    17821 tgcaggaaca aagaatataa aagcaaacaa taaccaaact ggtatagaac caagacccaa
    17881 tccaaaacta gaggcagtag atttagctat ccatctaatc ccaaatagtg ctgatatatt
    17941 cataaatact aaatcccaaa atccaatttt ctttttcact gtaattcctc caatttcaaa
    18001 tttattcaaa ctcatacaca atgtattgat ttaaatatca agtataagtt cttcaacact
    18061 tcttggataa ttagttataa ttgtagcacc attcttacct actattgctg tatctgagtg
    18121 tctaaatcct ccaactccag gtatatatat ccctggctcc atagaaaata ccattccttc
    18181 ttctaatatt aattcattgt caaatcttaa atatggctct tcatgttctg ataaacctaa
    18241 accatgacca attctatgat ttgaatataa ctctagacca tactttgcaa caacagctct
    18301 tgctgcttca tccaccattc ttgctggtat accagctttt attgtatcta atccagcttg
    18361 ttgagcctct actgcaatct taaacacttc tttttgtctt tctgttggtt tacctataat
    18421 aaaagttctt tcattttctg ctctatagtt ttcataccaa acctgtctac tatgtattac
    18481 tatatctcct ctttgaagta tcctagtatt ggaatctaaa tgaggttgtg ctgttctatc
    18541 aatccctgaa caagtccagt ttgcaaatcc tatatatgta tcaggataat tttcagaaac
    18601 atattttagt aaagcattat cccccgcaac gtcaaactcc aattcactca tcccaactct
    18661 aacattttct aaagaccctt ttattcctat atcagatagg taacctgcta tcttcaaaaa
    18721 ttctatttct tttgcatctt ttacatatct catttcaaat attttacttc caacatcttt
    18781 gatagcaaat gacttatcag ttatatactc tgaaaaactt gcagatacta tatctttttc
    18841 tattccaaca gttgttcctt tagggtattt atttaaaatt atatctaagt aatgtttatg
    18901 tgatattcca tgttctttca tttctggtat ttcataatac acatatacat tatcaacttt
    18961 tgcaacttct ttagcatgta attcttctaa tccaggtact attaattcta ttttatcttg
    19021 agttactact gttacaattg gtctagaata agttattgct ttaaatccac taatataaaa
    19081 ttgattgtct ggttcaaata ttaaagcaat ttcaatgctt ttttcattca tgtactttct
    19141 caatctttct agtctcatat tatttgacat aatgcacctc tttcttaata ttcagataat
    19201 ttattcaata caacaaggat aaatatatta ttttaaaatt cttaatattt ttagtacttg
    19261 tcgtatttgc atgtaaatta attttatttg tgattatttt atcataatca tgattatttt
    19321 gtcaatcacg attttatttt taaaattttt ttatattcta gtgattttaa attatctaaa
    19381 atataacgag tatacattgt attttggaat attattcttg atttatggta agtttaatga
    19441 taaaatcacg attatataaa ctatattgat tatttgtagg tgaataaaat gacaaataaa
    19501 gaattacaaa agaaaaggat attgatgtat ttcatagaag ctgctcaaaa tattatggaa
    19561 aacgaaggta tagaaaatat tacacttcgt aaggttgcag atatggctgg atataatagt
    19621 tcaactctat ataaatactt taaaaattta gaccatttaa tatcatttgc atctataaaa
    19681 tattttaaag attataactt gaatatttca agatgtatag aaaatgtcaa tgatgaatat
    19741 aagaaatata taattatgtg gaaattattt tgtaagcatt cttttgacaa tgctcaagct
    19801 ttttatcagg tattttttaa cttgtctagt gatgaactta gctatataac aaaacaatat
    19861 tatgatatgt ttccagaaga tttaggtatt catgatagtg acatatcttt aatgattaca
    19921 ggagaaagta taaaggaaag aaataagata ctattaaaca atcttgtaga tactggatat
    19981 ataccagcta aagatttaga cataatcaat gatataatta tttcttttta tcaaaatcta
    20041 ttattcttaa aaaagaacac aggaaaaaat tctggtgatg atgaattaac tatgaaaatg
    20101 atttcttcta ttgaatacat aataaaacat gctaaataaa attaaaatat ggctgtctca
    20161 aaatagagat aagccttttt tattgttgaa ataataaaaa tatatttaat ttcatataaa
    20221 aaaaagtgcc tcgtaaacta aaaagttcat tttgagacac ccctaaaaca aatttattat
    20281 ttattataca atagtttata tatcatcacc taaaactact tcttccattt catgcttttt
    20341 acttctcatc attagttcta ttccaacttc tttaggattt gaacctttat ttattacaga
    20401 atatatagca ttagttatag gcatatctat atctaatttt tcagcaacat catgtgctac
    20461 ttcagtagca gttatgcctt ctacaaccat ctttacttct tttaaagtat cttctaaact
    20521 cataccttta cctatgagta taccagctct tctatttcta ctatgcatac ttgtacaagt
    20581 aactattaaa tctccaatac ctgaaagacc tgcaaaggta gacatattag ctcccatagc
    20641 aattccaagt ctgctcattt cactaatacc tcttgtcata agggcagctt ttgcattatc
    20701 accataccct aatccgtcac atatcccagc tccaaatgct ataatatttt ttaatgctcc
    20761 tccaagttct actccaacta tgtctggatt cgtataaact ctaagtttag ggctcataaa
    20821 taaatcttgt atcatctgtg ctatttttaa atctttagaa gcaactacta cggtagtagg
    20881 aatatccctt gctacttctt cagcatgaga tggcccagat agtattacat atggattttg
    20941 tggtaattct tcttcacaga cttgagataa tctaagccca gtcccttttt caagaccttt
    21001 agctacatta acaataactt gtccttcttt tataaatggt tttatttttt ggcaagtact
    21061 tcttacagcc tgtgatggaa ctgctaaaac tattacctta caatctttta tagttttttc
    21121 tatatcagta cttatagtta tattgtttgg aaataatact cctggtaaat agtctgtatt
    21181 ttctttagtt ctgtttattt ttttagcttg ttcctctttt cgagtccaca tgctcacatc
    21241 attacctttt tttgcaagcc ctaaagctaa tgcacttccc cagcttccag tacctaatac
    21301 acatactttc tccatgttta acaccacctg agtataatat atttactatt ctttcttttg
    21361 accaattttt ctctctgtcc catttaataa tcgctttata tttgctctgt gattatatat
    21421 tactgatgca gttaagaaca gagctactat taaaccagct ttatttttta ccataatcat
    21481 aaatattggt gaacatccta ttcctacaac agaacccaat gaaacatact ttgttatagc
    21541 taccaccaat ataaatactg ctagacacat tagtgttatt actggattta cagctagcat
    21601 tgcacctagt gaagttgcaa cacctttacc tcctctaaat cctaaaactg ctggccaatt
    21661 atgacctgct acaacacata taactgccag atatccagct aaaagagtat ctattccaac
    21721 caatcttgct gcaaattctg atattaaaac agccattaca cctttaagaa catcacctaa
    21781 aaatgtcatt gctcctgctc tttttccaag tgtcctaagt acatttgtcg aacctgcatt
    21841 accagaacct tgagtcctta tatcaacccc agcaattcgc tttgcaacta tgtatgaggt
    21901 agatatattt cctaaaagat atgcaactac agcaataatt atataactaa atatctccac
    21961 tgaatttccc ccctttagtt atattttaag atatttattt tttatttttt tgtctatact
    22021 caaactttat ggaagttcct tcaaatccaa aattttctct tatcttattc tcaaggtatc
    22081 tttgatatga gaagtgagtt aaatccttat catttataaa caatgtaatt ttaggaggtc
    22141 taatgtcagt ttgagtccca tagtaaattt ttagtcttct acctttgtca gatggtggtt
    22201 gatttaacat tacagcttct ccaattactt cattcagtgc tgaagttgat attctcttag
    22261 aatgttcatt tgacacatat tcaactgtat ctagtatctt gttcattctt tgattagttt
    22321 ttgcagaaac aaatacgatt ggagcataca tcataaatgg aaatttttct tttatatctt
    22381 ttgtgtaatt actcatagtc ttattatctt tctctattaa atcccattta ttgattacaa
    22441 atatgcatcc ttttccttca tcatgtgcta tacctgcaac ctttgtatct tgttctgtta
    22501 caccttctaa ggcatctatt actatcaata ctacatctgc tctatctaca gcactcatag
    22561 ctcttattac actgtatttt tctattgtct catatatttt actttttctt ctaagccctg
    22621 ctgtatctat tagaagaaat ttctgtccat ttttttcaaa ataagtatca attgcgtctc
    22681 tagtagttcc tgcaattgga cttacaatta ctctttcttc tccaagtatc ttattgagta
    22741 tagaactttt tcctgcattt ggtttacctg ttatagctac tcttattata tcttcttcat
    22801 actctgtatc taatcctgct ggaaaatttt caactatctc atctaataaa tctccaaatc
    22861 ccatactatt ggctcctgaa actgcaaaag gcgttccaaa tcccaactca taaaaatcat
    22921 atatattatc aaattgactt tgactatcaa tcttattaac cactaagatt actggttttt
    22981 ttgtctttct aagcaattgt gcaatttctt tatcagcagc tgttattcca gctttaccat
    23041 caactataaa aagtattaca tgtgacatgt ccattgcaag catggcttga tttctcattt
    23101 gagataatat tatatcttcg ctatcaggtt caattcctcc tgtatccact aatgtaaaat
    23161 atttatctaa ccactctact tcagcaaata ttctatctct tgtaactcca ggtgtatttt
    23221 caactattga tatccttttt cctgcaaatt tattaaatat tgtagactta ccaacgtttg
    23281 gtctcccaac tacagctaca actggcctac ttatactcat tttatttctc cttccatctt
    23341 ttgttttcta ttttaaaatt ttatccacaa agtcttttcc ttcattcaaa caagggataa
    23401 tttttattct cataagttct tctaattgat ttaattcaat attatctaag aaaatttctt
    23461 catctgcttt tagcatactt cttgttatat aaagagcttc tcctaattct ttatcttcaa
    23521 gttgttcttt taaatcctta gctgttaaaa gacctgatac agttattgtc tctccaaaaa
    23581 acttgttttt gatttcatat acatttactt gaagattttt aaacttgtcc atcatagcat
    23641 ctgccatact ctgtataaat tcatatgcag aatgaccagt agctattgat actttcttct
    23701 ctctttttag aatattctca ggcaatatat ttagatactc ttcaatctct gtttttaact
    23761 tacgtatcat acctacacca tcttcaaact gtaaaaaacc ttcatattct tcatatccag
    23821 gcaattcact attagatagt atataaaatt catctgataa aaatgcaaat cgagtaccta
    23881 atttttcaag atatttctgt tgtatttgat gtatttgctc tatagtttta cttgcgctct
    23941 tatcattaaa tatatttaat tctactagat tttctctatg ctttgtaatc ccaactggaa
    24001 ctatcgcaac actgtttaca tatggataaa gttgagctag ttcttttatt gttctatcca
    24061 attcctttcc atcatttata cctggacata aaacaatttg acagttcatt tctatatgag
    24121 catctgccaa tctcttcatt ataccatata attttcctgc aaatttattg cttatcattt
    24181 tttgtcttaa ctctggatta gttgtatgca cagatatatt tataggactt attctatatt
    24241 ttataatatt atttatatct tcttcactca tatttgtaag tgtgacaaaa tttccttgta
    24301 aaaaagataa tctcgaatca tcatctttaa aataaagagt ttctctcata ccttctggca
    24361 attggtctat aaaacaaaac atacacttat ttctacagct tttagcttgg tcaattatcg
    24421 gatttgtaaa ttcaacaccc aaatcttcat catagtcttt ttctatctcg tatatgtaga
    24481 cttcgccatc ttttttttgt atttctacat ctaaatattc atcacttagt aagaatctat
    24541 actctattat atcatgtatt ggctgttcat tcacagatat aagtaaatct cctacctcta
    24601 tacctatttc ttctgcaata ctatctttat aaaccttact tataatatta tttacttttt
    24661 ttacatttac ttccaatatt ttcaccactc ttttgtattc atcaatgctt attataatat
    24721 attttaatat gcaatttatt tatttattat attttatgat agcattttta agcatttcat
    24781 gtcataataa cacattttaa ttctaatagt caatataagt aaatagctat aaaatagatt
    24841 agaacaaata atattcaaat ttaagtttct taaaaatttg aatattattt gttcttaaaa
    24901 aaattttata aatttaatgt tttactatga ttaacgtccc atttgtcata tcatgaacca
    24961 ttccacctac acactttgac atcaacaatg ccttattttg taagtctttc tcatcatttg
    25021 cataaaatat tggacaacct ccaccatttt tcatattctt atcaagtgta actatagcta
    25081 gggtatattc atttaaacct atatccattc ccatcttatg cacttccttt ctttcctatt
    25141 ttataatttt ttagtgataa gttttttcct ttagagcttg aaagtattgg gcatgacttt
    25201 acagcttcca ttactttttc catattctta tctactggta aataagcaat cattatactt
    25261 tcatcctctt tatttcttct aggtaatggt acgaatgaag gttctccata atctccatat
    25321 aaacctaacc ttgaatatat attgtatact attgcttgtc tttgaccgga gtcaaataat
    25381 attcctgcat tagcataatc cttattttta ggaattattt gtattcctac acctctatta
    25441 atatatactt ctctatcact ttttagacct atatttgtta taccttttaa atctcccacc
    25501 tttagtatag attcgtccac aaaactaatt tcagcaggca ttacatcagc tatgtctcca
    25561 atactttttc tagtaagaag tttttttaat ataaaggcta acactaatcc acaaattgaa
    25621 cttattacca aactcatccc ttcacttatt ttcgtttctg caagtattac ataatacaat
    25681 cctattgtta aaaaagatgt aactatacac atataatttc ttacttcata ggttctagct
    25741 atctcttcta tgaatgcagc acctctcttt acaagttgaa cttcctctaa attttgcaat
    25801 gtatttcttc tatttgctct cacttgtcta aattgttctg ctgctagaga taaaaatgtt
    25861 atggatgtat aagaacgatt tataagagct ggaaccaata atgctcctaa tgaagaagcg
    25921 acgaatgcta atataagttg agatagtaaa acatttggtt gcgttggata ctgtttttgg
    25981 tctaaattca acattataat tctagatgtt ataccaataa taacagcaac aaaaaatact
    26041 ctatccatta aatcactcct aaaatattta atttgaaatt ttacatgtaa atctacaaag
    26101 tctttctaga aaaatcactc tgctttcctt ttgatgtttc tatgataggt gtacttttca
    26161 ctacatctat catgacatct atatctttga gtattggcat atatggtata accacagtac
    26221 ttttttctaa attggtctta gatatagcaa caatatcaac ctcatctaca tctctatcta
    26281 tccccatatg aataaataga ttatgtatta tagcttgtct ttgccctata tcatttacta
    26341 ttccaaatgc acctaaatct cttggtataa cttctatggc cagacattca tttctatact
    26401 tttctcttgt actttctaga cctatattag ttattattac atcatttacc attaaaatag
    26461 gtccttcaaa acttattttt gcaggaacta tatctgctat atctgctata ctgtttcttc
    26521 ttaaaaatct tctaaaaatc aagccaacaa tagctcctga cacaatagct aaaattgtgc
    26581 aatgaaaaaa gctaaggtca tactttcttg caaaaacgat ataaactatc gaagatacta
    26641 gtgcagaaaa aagacttata taacttctag attcatacgt agatgctatt tcttcaacat
    26701 aagcatctcc ttttggtact aattcttcat tatcaatatt ctttagtgtt attctttctt
    26761 gttccgcaag accttgaaac tgttgtattg ctactgcaaa aaatgtcaag gctgcaaact
    26821 ccttatctat aagtgcaggt aatgctatag cacctaacga agctgaaagc ccagatataa
    26881 ttatctgttc taaataatct tgtggtcttg atgggtattg tttgtctgta actcttaaaa
    26941 ctaatcctct acataatatt cctacaacta atgcaactat aaaagagtgt ctaaataaat
    27001 ttgcattcaa taaactttct tccattttct cagctccttc tcttatatag tttgtgcaaa
    27061 aaacaaaaaa aattcccttc tatttaagga aatttttttg taatattgta actataaatt
    27121 cgctaccaat gccaagttca ctttttactt ttatacatcc atttagtgat ttgactatat
    27181 gctttgttat agccaatcct aaacccgttc ctcctacatc cctacttctt gctttatcta
    27241 ccctataaaa tcgttcaaat attcgttcta catcttcttt tggtatccct acaccattgt
    27301 ctataacttt aattacgatt ttttcttcat cttttaacac tacaacttgt acaatcccat
    27361 tatcttgggt atactttata gcattatcaa ctaggtttaa gaacacttgc tttatatagt
    27421 ctttattgga tagtatactt attgatttat catcacattt atataaaact tctatatttt
    27481 tttcactagc tgaatgttga gttatttcat atacctcttt aaatacatca tatactacaa
    27541 cattttccat aagaagagta tctttatttt ctacaaatga taacagcaat atatcatcaa
    27601 tcaacctttt taatctatct gattcgcttt ctattatttc taagaatcta tttctagtat
    27661 tcacatctat attttcattt gactttagtg tttcaacaaa cccacttata gatgtaagtg
    27721 gtgttttaag ttcatgactt acatttgcaa caaaatcact tctcatattt tcaagtttaa
    27781 cttttttagt aatatcttct atatttacta tcgaacctat tattacgttg ttagtattct
    27841 gaagataaac tgggtctacc tttatcttat atacgatatc atctttagta gtaatattag
    27901 cagtttcatt atctttagaa ccaataaatt gaagtatttg ttttaatatt tcttcttctt
    27961 ttattacaac attaatatta tgaccttcaa ctaattgatt tggttcactc tttatcattt
    28021 cttttgcttc atcatttatc agaagtatat ttccatttac atctatagcc aaaattccat
    28081 gagatatact ctttaatata gatcttaact gtaaatgttt atactgtact tcttctaagg
    28141 ttttatccat attctttatc atatgattaa aatttctagc taattcacca agttctcctt
    28201 tggctgatat gttaagccta gcatgatatt cattattact tacttttttt gagacgtata
    28261 taaactcttt taaatatttc cttaatgtta ttgtatatct tacagctaat ataacaatgg
    28321 ctatggacaa cataacaata aaaaaatata tactatttaa atcttccaca agataacact
    28381 cctaaaatat aaccttttta atctatttta taccctacac ctcttatagt ctctatatat
    28441 ttttccgatt tatcttcatc ttctattttt tttcttaaat atcttatatg aacatctact
    28501 gttctagttt ctccatagta ttcatacccc catattttat ccaaaagata atttcttgaa
    28561 agtacttttc ctttattttg aattaataat tttaacaatt caaattcctt aagagttaac
    28621 tctatctttt cagagccttt ggtaacttca tgttttgata aatctagttt taaattgcct
    28681 gtagtaagta tattgttttc ttcacctaag ctagtcaaat tatatctcct taaaacagaa
    28741 ctaatcctag ctaacaactc ttttatacta aatggcttag ttatatagtc atcagcacct
    28801 atttcaagcc cttcaacctt gtcattttcc atatttttag cagtaagcat tataacagga
    28861 atatttttta agtccttatc acttcttatt tttctcaaaa cttctattcc acttatattt
    28921 ggtaacatcc aatctaaaag tattaaatct ggtttaattt ccttagcctt tataaatccg
    28981 tcaaatccat catatgaata actaacttcg taatttgaca cctccaaatt gaattttaat
    29041 agctcaacta tatgcatttc atcgtctata acaagcactt tagtattcat taaaaagctc
    29101 ctctctattt tatctgtagt accgttccta ttcgttctga tgccttatta tgttttctat
    29161 aagagtggaa tttatctgct ctacaactag tgcataaatt taaatttatt atattttctt
    29221 ttttaactcc actacatcta agcatatact cattaatttt ccataaatct aaatagtaac
    29281 ttccctcttt tattatatag aacttttcat cattatttgt aagaattgtg ttaaattttt
    29341 ctactaaatc ttcagacact tcatagcaac atggtccaat tgatggccca attatacata
    29401 ctaaatcttc aggaactgtt ttatagcatt cagacattaa ttttattgtt ttatgaccta
    29461 tattactaaa agtccctctc caacctgcat gagccagtcc tatggcttta tttttagcat
    29521 ctataattga tataggtaca cagtctgctg taaacagtaa aagtggtact ttttccaagt
    29581 ttgttataag tgcatctcca tctcttcttt tccctatagt atctttattg actatattaa
    29641 ctatatctga atgtatttga gaattactag ttatattttc aaatgtcaaa tcacattctt
    29701 tacatacttt taacatatct tctctatttt ttgcatctat attcttacat gttataacta
    29761 gatttacaga atttaatttt tcatcaagtt cttcacaatt accacttaaa ttaacatact
    29821 ctttaatatc ttgtacattt tttatattta tataatcttt catcatttgt ctcctttttc
    29881 tatcagtata cttttaagtt catttacaaa agtatttata tctttaaatt gtctatacac
    29941 agaagcaaat cttacatagg caacctcatc taactctttc aatttatcca ttaccatctc
    30001 accgattttt ttggtttcaa tttcttctat aaaacattta tttatttcat tttcaatatg
    30061 tacaacaata ctttcaatct cttcaataga aacaggtctt ttttcacatg cttttagcaa
    30121 tccatatttt atcttttctc tatcaaaata ttctctacta ttatcctttt taatgaccat
    30181 taaaggagtt gtttctattt tctcatacgt agtaaacctc tttttacatg attcacactc
    30241 acgtcttctt ctgatagatt ttaaatctgt atgtctagaa tctattacct ttgattcttt
    30301 ataattacaa taaggacatt gcatctatat tcctcctttg tttaactata tctcaaactc
    30361 atctaagctt aattctgaac ctatatttac tattattgta tcacaaccta ttttaagtat
    30421 atcattccaa aatataactt cttcatcccc tcctcttgaa aaaaaacttc tacttccatc
    30481 tccaaaaatg gtaaatgaga ctaccttacc ttcgtgaata tccatgtcta tgtctattat
    30541 aaaccccatc ctttttccat tttttacatt tataatttct ttttccatta tatcagaaac
    30601 tctaatcata ttgtactcac cctttcttta aaaataaata tgtatcgaag taaaaaaaaa
    30661 tgacctatat aggtcattta aattaaaata atgtttacag tccatggatt acttatacat
    30721 attttctcat attttttaaa gcattttttt caattcttga tacttgtgct tgagatatac
    30781 cgatttcatc agcaacttct atctgagttc ttcctttgta aaaccttaaa tctaatacca
    30841 acttttctct actatttaat ttcttaatag cttctttaag tgatatttct tgcaaccaat
    30901 tttcatcagt atctttttta tcttgaacct gatccataac aaatattgca tctccattgt
    30961 cttgatatac tgggtcaaac agagatattg ggtcttgtat ggcatcaagt gccattacaa
    31021 ctgattctac ttctaattct aattcctttg ctatttctga tactgtaggt tctttagaat
    31081 ttgttctaat taatctttct ctaacttgta gtgccttata tgctatatct tttaatgacc
    31141 tacttactct aattgggtta ttgtctctta gatatcttct aatttctcct ataatcatag
    31201 gaacagcata agttgaaaac cttacatttt ggcttaaatc aaagttatct atagctttta
    31261 taagaccaat acaacctatt tgaaataaat cgtctatatt ttctcctctg ttgttaaact
    31321 tttttattac actcaagact aatcttaaat ttcctcttac aaactgttgt ctggcttctt
    31381 catcaccatt ttttatttgt aataaaagtt ccttcatttg cttattttta agaactggaa
    31441 gttctgatgt attaacacca catatttcaa ccttattaac ttgcatatat tttcagtcct
    31501 ctctcaaaag tattcatcta ttgaaattat ttacatttcc aattttttta tactttttaa
    31561 gaagaattaa tttaacatta tacaaatttt ttcatttctt tttgcaacct tgagattatt
    31621 ttcttttcaa gtctagatat gtaggactgt gatattccaa gcattccagc aacttctttt
    31681 tgtgtttttt caataccttt atcaataaga ccaaatctta attccataat ttgcttttct
    31741 ctgtcactta atctgtcaag tgccataacc aacaaatctc tatctatttc ttcttcaatt
    31801 attttgtata tttcatcatt ttctgtaccc aatacatcag ataataaaag ttcatttcca
    31861 tctaaatcta tatttaaagg ttcatcaaat gatacttctg ttttcttttt attatttttt
    31921 cttaagtaca ttaatatttc attttctata catctcgaag cataagttgc caattttata
    31981 tttttattca gtttaaaagt attaactgct tttatcaaac ctatagttcc tattgaaatc
    32041 aaatcttcta catctatacc tgtattttca aattttctcg atatgtagac tactaatcta
    32101 agatttcttt ctattaaaat tgactttaca ctttcatctg tttctagctt ttgtaaaagc
    32161 tccatttctt cttctggttt caatggagga ggaagtatat ttgcccctcc catatagtat
    32221 attccctttg gctttattat ttttaattta atacctaaca tagtaataaa gcttattatt
    32281 ctttctttca atcgtaacat tccttgtccc ccttaaattt tcacctaaaa tccaggcaat
    32341 atatttggat tcattattgc tccatattct ctatcattaa aatttgaaat accaagtatt
    32401 atatttccta tcttttgctc atctacttct atatagtcag cttttaagcc tattattatg
    32461 cttgtcttat tacttccagc atgtttataa ggtattattc taactctacc tgaagtttta
    32521 tcatctaaac ttcctataat ttcttgtgct ttcttcatat ctatatcttc ataattaata
    32581 tttaatagtt tttctggaat aaatccttct aacacactag acttaactat tattacatca
    32641 ctttgactta ttgggtcttt taacaaatta ccactatcta ctaaagcttt acagcaaaaa
    32701 cttttttcta gtaagtttat atttatagtc tttgtaagct ccttaatgta cttaagcatt
    32761 ttcaaatctt tataaatgta ttttaataat tcacaactca cataagtaca tgcaattaag
    32821 aaagatattt ttaagtgact tattcctgta aaataaatta taaaataagt gcttccagaa
    32881 ataaaaatgt ttactagata aaaaactaac gctacatgaa cataatcttt tttgctcttg
    32941 cttctaaatg aaattaaagt tattatgctc atgattatta ttttaaatgg tacagtaaat
    33001 agtatattca ttgatggata caaataagca acagaatagg cacttcctaa actagccccc
    33061 aataacttat tttttttagg attatatctt ttagtaagaa tagaagtaca acttataata
    33121 atataattta ttactaggtt ttctataaca tagtactcaa tatacaccat gtatcccccc
    33181 ttaaaacctt taaacatatt ataagatagt atgtacaaaa ttttatgtca cttttgatgg
    33241 tcgaatccaa aacaaattct atttaaaaag aacaatgata tatgtcttat actttatgtc
    33301 gtcaaaagat atttttttaa tgccaaacag atagactttt ttgtagaatc caatatatct
    33361 aggtagaagt tattcgcttt acttttggta tatttataaa aaaaagacta cataatttaa
    33421 taatatatca agtaaatctt ttacttatat aaaatcaaat tatatagtct gtaaattcaa
    33481 atataaatta ttgtgctagt aatccatttg catattttgc agattcattt agtgcttgtt
    33541 ctggagttat gtctccagct atcatcattt gaagttgttt ccataaatat tcatatatct
    33601 ctactccatg aggtccaact gtcaatggtc tatacatatg tttatctctt gtcaccattt
    33661 cttcaaatct tgggtctcct tggtattttt cacctttagc aactggagct ctaggtattt
    33721 ctttgtggaa agcttccatt gattctttgc ttaacatata ctcaagaagt ttaaatccaa
    33781 gttctttatt tttacatgct gacattaatg ttaaatcatc tactgaagca aaacttccaa
    33841 ctgtcttatc ttctaatgat gttacatatc cccagtttaa atccgggaat gatttatcaa
    33901 atgtacttgt agcagcagag gatagcatta tagtaaatgc agcttttcct ggtccaaata
    33961 cactttcaat catttcatta ctatcttttg ataaagcatc ttctggaata tactttttca
    34021 tatcatttat aaagtttgca gctttcaagc cttctgcatt attaaattta actgatttta
    34081 aatcatcatt atatatatct ccaccagctt gccataaata cggataccag ttccagttta
    34141 agcttccaaa tactttagaa ccccaacctt gtgcaagacc ccactggtcg atttttccat
    34201 ctccatcagt atcttttgtt gctttttcac agattctttt aaaatcatcc catgtttttg
    34261 gaggtttttc tcctaatttt tctagtatat ctttattgta gtacaatacg cctggattag
    34321 cagcttctat aggtaaacca taaataccac ccatcatttt tgcatcatct aagtatagat
    34381 aattatctga agttcctgat ttttctaaat aaggtgttaa atcttctact gcacccattt
    34441 ctataaattg agggaacatc tctgcataca tgtatcctat atctggacct tcacctgcac
    34501 ttattgctgt tgcatatttt tctgcgtagt tatcccatgg tactatctga aattcaattt
    34561 cacagttatt ttctttttca aatttttcaa atatcggctt ccatgcatct gcatcatggt
    34621 ctgttaatgg tgcagaccat acaactaatt tttctttacc ctctttgcct ttagaatctt
    34681 ctttttttga acatcctgca agaattgata ctgatagaat ccctaccata actaaagaaa
    34741 gaaccttttt tagtttaatc atgcttaatc ccctttcaga ctttaatttg tctttttcct
    34801 caaatgttta catttaattc agatttaata ttaacaaata agactactct tgtctaatca
    34861 taaaaaacta tactaactcc tataactata ttttttttct atacctatta gatttaattc
    34921 atactaaaaa cttacttttt aaattttatc aatatttaaa aatattttta agtctagcca
    34981 caaactaact atctaaattg aacaaaataa aaagaagtct attgacttct ttataagctt
    35041 acatattatc ttcttcttct taggaatgta ggtatctcca tgctctcatc atcatcttct
    35101 tcaattatac ttgccttttt aggaggctct attttttcct cagcaattac ttcttcttca
    35161 atttctttta cagcttgttt tactgttgta tttaatgaag aacgtatgct tgatttaggt
    35221 ttagtatcta aatctaaggc accattttgt aatccttcaa atcctgttgc gattactgta
    35281 atcattattt catcacctaa atcttctttt atagaagctc caaatataac atttgcttct
    35341 gggtcacagg attccataac taaagttgat gcctcattaa cttcaaataa tcctaagtta
    35401 ggaccacctg taacattaag aagtactcct ttagctcctt gtattgatgt ttctagaagt
    35461 ggagattgaa tagcttgtct tgcagcatca attgctctag tttcaccact agcactacct
    35521 atacccatat gagcaagtcc cttgtctttc attatagtag taacatctgc aaagtccaag
    35581 tttattaacc cttcaactgc tataaggtct gaaattgatt gtataccttg ttttaataca
    35641 tcatctgcaa ctgcaaaagc atctaacatt gatgtatttt tttgtactat ctgtaaaagt
    35701 ctatcatttg gtatcgttat aagagtatct acttttgatt taagttctgc tattccacct
    35761 tcagcgttct tcattcttat cttaccttca aatgcaaaag gtttggtaac tacacctact
    35821 gtaagtatcc ccatttcttt tgccaagcct gcaaccactg gagcagctcc tgtaccagta
    35881 ccaccgccca ttccggctgt tacaaagacc atatcagctc cttgaagtaa ctttactatt
    35941 tcatctttac tctcctcagc agctcttttc cctacttctg gatttgctcc tgctccaagt
    36001 cctctagtta atttttctcc tatttgaact ttatattcag cttttgatgt atataaagct
    36061 tgtttatctg tatttactga tataaattca acgcctttta gttgagcttc taccattcta
    36121 ttgacagcat tgtttccacc gccacctaca cctattactt taatttgtgc acattcttct
    36181 aattctacgt caaagtttag catcataaac ccccttctct catgattcaa agttatcgta
    36241 tgtattaatt atatcatttt cttcttttat tttgttaacc tttaagtaac atattccaca
    36301 aaaacgtaaa ttttcctttt taaatttctt acttatattg tcaaatatta ctctttaagt
    36361 ataaaataat ttacgtatat attaaatgct ctttaatttt tacgtttttt gttcataata
    36421 ttactaataa tatatcgtct aattatagat aagttattaa acaatctcat accaaaaact
    36481 agtacagcaa catagtaaag agaaactcct aacttttctc ctatataagt caataatata
    36541 gccaatatag cattacctat gaaaccagta ataaaaatta cattatcata gttatcttca
    36601 aaattggatt ttatcgctcc aaaaatagaa tccattattg caagaatagc tactgacata
    36661 tatatggaat aatccataga atatgtaaat ggaatatagt aacctactac tacaccaaat
    36721 gctaatcctc ctaaaaccca caacataaat cttcacctct ttattttact ttgaaacaca
    36781 actacattac aatccttttt attaattttt tttacttttt ctttattttt ttgtattttt
    36841 cgttttattt tgcaaataaa ttcactttat tcccattatt ttcctgaact tttatattaa
    36901 ttccatatat atccttaaga gatttagcat atgattctgg agagtttata gctgcactca
    36961 ataaatctat atctcctata gcttttatta taaatggttg tccatatgta gtatcattta
    37021 ctgtaatagt agctcctgaa cattttatct tacttgttgc tagtactctt tccccattta
    37081 tagaaattgc tcttgctcca gcctttttta aatcatttaa aactctcaaa atatctatat
    37141 catgtataat caaatcattt ggattctgtc cgtcttttaa ctctctctca ctatccctca
    37201 tagtaatagt tatgcctggc cctgtaacag ttgaataacc acttaattct ttaacttctt
    37261 caagctcttt ttgcataagt gtttggatag attcatttcc attctttttt gcttctttat
    37321 attcattcaa agtttttttg ttgtttttaa gagtttcttt taaatttttt atttcttttt
    37381 ttgtattttc tacctcactt tcaatacttt cagtttgacc taatgttatg tatactctat
    37441 ttgggtctag tgattttata taagttgata tgaaaatgcc taatatgaat gctcctaata
    37501 taactatttt ctttttcata ctattatttc ccctctgctt tgtttatatc actatgacca
    37561 tttatttcta tattgtcttc tttggtcact tttactttgg cattataata tgtttctatt
    37621 tcccagacaa taccatactt tatattaagt gccgaagcca atgtatcaga atcaccaatt
    37681 gcttttatta taattggttc ttttatgaca gtgtcattca tacaaagctt gtcttctttt
    37741 aaatacatat atgtattaaa aactattctc tggtcgttta tagatatggc acttgcttgt
    37801 gctgaattta atttatttat cattgacaat aataaatcat aattatatag tatactatca
    37861 tttttatcag agtttcctaa gtcttttgta gaatttatct caatttttat gcctgttcct
    37921 tcaacatctg tatatccagc taatgtttca tatttttcta tttctgactt aagtatgtct
    37981 tgttctcctt tattgttttt gcctctataa gtctctatat ctttcttcac actattaact
    38041 tctgattgta gattctcttt ttctgttttt aaagatttaa gttcttctgt aagttgctct
    38101 cctcttttaa aagttgtcat ccctctattt tctatattaa taattttaag ttgtaaacta
    38161 acaagaacac ccaatacaac acttcctatg attatacttt tatttataaa tctttttttc
    38221 aatatatcac ttccttacaa ttgtctatcc ttctggacta tacactgcca actttccata
    38281 gttaagattt atagtccctc cattagtatt tttattttgc aggtcaatca gaattttact
    38341 taccctagat atattataat ctaagttatc atcattagaa agtaagattt ttatattact
    38401 tcttgtaagc atttctatat tgctttcttt ttttaagttt acataattta tttttctata
    38461 aattccttct tcttttaaat aatttaaagt tttaaataca ttctccttag ttttataatt
    38521 tttaaatttt atagatttat tatcatcaac gctataatct acatctacta tcaagtcttt
    38581 tttactttca ttagaacctc ttagttcttc caataaattt ccttttttat ctatataaca
    38641 ataattatct tcatctttta atactgcaaa tatttctttt tcttttaaac ttataattat
    38701 cttattggga agttttcttt ttatttccac attttctata tatggatttt ttataagttt
    38761 atttttcata tctttaaaat tgtatgcaaa aatattttca tttagattta tatttagttc
    38821 tttcattata tttgatttgg tcaccctctt atttccaatt acatctattt tctttacatc
    38881 aaataaatca ctttttacaa ttatacatat acaaaaagat aacataagta taaaaactaa
    38941 aactatcatg acattattcg tatttaactt tcttcttttt ttcaaatata atcaacccct
    39001 ttggttaata aatacacctt tagagcttgt ttttcctaca aaaaaattat aatatttaaa
    39061 gtcaaatttt tgcattttat tacataacat ccaaataagg tattaaattt tataaataaa
    39121 tcaatatttc atcaaaataa acatagtata ctacatacta tttatatatt taaatctgta
    39181 tacatacaaa taaaccccta atttataata atttagaaaa tctaatatat tataaattag
    39241 gggtaaatat ttacttatct tttctttatt cctattacct tagcttgacc ttctatactt
    39301 ggagtcgttt ctttttttat ttcttttact tccttaataa ctttttcctt ttttgttttt
    39361 ttagaagttg acttttgtgt tgaattgtat actttcatta tctcatcata tataaggtct
    39421 atggcttcag gcttacctat agttttactt gcatttgcca tatcaactaa aagttctcta
    39481 tctcctaaaa gtttaaatac tgctgtattt aggctctctg gagttagatt tttttctaaa
    39541 atagcaattc ctgccccttg tttttctata ctcttggcat tatactcttg atgattttca
    39601 gcagtataag ctttaggtat tattatagat ggctttccaa gagccgttat ctcagctaat
    39661 gaaattgctc ctgcgctacc tataaccaaa tcacttgctg caagtgcatt tgccatatct
    39721 tctaagtaag gcacaacttt ttggtatggt ttaagattta tatcgctaat acttcccata
    39781 aattcatcat aatagctttt gcctgtagca aatataaaag ctatatcttc atttaccata
    39841 ttctttatta caagtctcat tgcatcattt atttttcttg aacctccact accaccatag
    39901 caaagtacca ttcttttttc gtcacttatt gatagattct ttcttgcaat attttttctt
    39961 gataataaaa tttcttttct aactggattt cctgtaaaaa ctaatttatc ttctgctgcc
    40021 tctggaaatc ttttatgcga atcttcaaaa cttgtaagta cctttgtaac tgttttagat
    40081 aaaatcttat tagttacacc aggaaaagaa ttttgctcat gtattatggc tggtattttc
    40141 cccatagatg cattaaataa aacaggtcca ctaacatatc caccagttcc tataactata
    40201 tctggtttaa atttcttaac aatctttctt gattgctcca aacctttaaa aagcttaaaa
    40261 actcttttta cattatcgaa atctattttt cgcttaaaac cttgaactgt tacagtcttt
    40321 aattcaaatc catacttagg aactatttct gactctattc ccttttccgt cccaacaaaa
    40381 attatctcag cgtctggatg ctcatctctt attttgtttg caatagctat agctggatac
    40441 acgtgccctc cagttccacc gcctgataat aaaactttca tcaaatcatc atctcctgtt
    40501 aattaatttt gacatgcttt gagatgttta acactattcc tacagctccc ataaagatgg
    40561 tgagtgaagt tcctccataa cttataaatg gtagtgctac acctgtagct ggcattgatg
    40621 atgtcgcaac tgctatattt aaagctgctt gtattccaat ttgtgcacct atacctataa
    40681 caaccataca agcaaataca ttagaagtct tcaaagctat tcttacacat ctgtagacta
    40741 aaactacaaa tagcataata actattatac aaccaatcaa tcctaactct tctcctatta
    40801 tagcaaatat aaagtcattt tgtggctctg gtatataaaa atacttttgt ttactctttc
    40861 ctaagcctag accaaacaat ccacctgaac ctagtgcata tagaccttgt attacctgat
    40921 accccttacc aagagggtct tgaaaaggat ctaaaaatga tgtaacacga ctaagtctat
    40981 agggttcagc aattactaat gcagcaaata aagcagcccc actccctatc atagcaaata
    41041 caattttcat atccattcca gcaacaaata tcattacaaa agtaactaga attacagtac
    41101 ctgctgttga caaatttggc tgaagtatta tcaacgcaaa aaatatccct ggaactacga
    41161 gaagtggtac aacacctttt gtcaaagatt ttattttatc ataatttttt tcaattaatt
    41221 ttgcagttaa tattattgta gcaaactttg cgatttctgc aggttgaaat gttaaagctc
    41281 ctattcccaa ccatcttttt gctccgtttg cttctatccc taaaggggtc aaaactaaca
    41341 ataataatac tactgctata gctccaattg ctgtagcatt tttcttccaa aaactataat
    41401 ctattcttga tgtaataatc ataactataa atccaagtac agcatatatt acattctttt
    41461 ttagaaaata gtacgcatca ttgtgtttaa atgaagattg tataaaactt gcactaaaca
    41521 ccataactat tcctacaaat actaaaagca tagtagtgta aaacactact ccatcaaatt
    41581 cagtcttcat ccttatgtca atttgttttt tcaaattttc tttaggcatc caactaatct
    41641 acctccattc ttccatttaa gtaagtttag aggtaagaga ttattttaaa ttgtttacat
    41701 tatctttgaa gtcttttcct cttacttcaa aactttcata catatcccaa cttgcacaag
    41761 ctggtgaaag aagcactata tctccacttt ttgaaatttc ataagaagtc ttaactgctt
    41821 cttccatatc ttttactata tgtatgtcat taaatccttt atttttagca caattttcta
    41881 tatttgatgc agtttctcct aataagacca atgatttaac atatgacttt gcagtttcaa
    41941 gaagttcatc aaaattactt cccttatcca tcccccctgc tataagtatt ataggtctat
    42001 cataagactg tatagctttt atagtggaat ctggattagt tccctttgaa tcatttacat
    42061 atattacatt gtctaaattt cttacaaact cttgtctgtg ttccactcct ttaaatgtca
    42121 taagtacatc ctttataact tctaaatcaa ttttgcagac atatgctatt gctatagctg
    42181 ccatacaatt ttctaagtta tgacctccag gtaaacttac atcatcttta tttagaaatt
    42241 tgattttatc atctatatct attattatgt cattattttc taaatatact cctccattta
    42301 cttttttagt tcttgaaaag taaattactt tggcattgca tttatcagct aaagatttta
    42361 ctacctcatc atcatagttt aacacacaga aatcctcaac agtttggttc ataaatatat
    42421 ttgcctttgc ttctatgtat ttttccatag tatggtgtct atttaagtgg tcctctgtaa
    42481 tatttaatat tgcgcttact ttaggtctaa attcatctat actctcaagc tgaaaactgc
    42541 ttagttcagt tactaacaca gactcttcac ttgaagtttc tatagtatct attacaggat
    42601 ttcctatatt accaactaca tatgtatctc gttttgctct tgaaaaaatt tctcctacca
    42661 aactagttgt agttgtcttt ccatttgtac ctgtaatccc tataaaaatt ggattattcg
    42721 atagtttaaa agctaattct acctcaccaa ttatatactt gcctgaattt tttaatttca
    42781 atatgaatgg taggtctagt ggtacacctg gagacactac aaccatatct atatcatcta
    42841 catcttctgg gtgatgtcct aatatgtatt ttatgtcatt tatagatttc aattcatcaa
    42901 gtatatttct caatttattt tcatctttta tatcatttac gattatactt gcacccaatt
    42961 tatctaaatg tttaattgta gatataccag tttttgcaag tcctactaat aaaacttttt
    43021 ttccttttaa gtccattttt cattccccct agccctaaaa tactgctatt atacttatcc
    43081 aagctaaaac aactgttata atccaaaaaa taaatacaac tcttgtttct ggccaaccac
    43141 actgttcaaa gtggtggtgt attggagcca tcttaaagaa tctctttctt gttgccttaa
    43201 aatatccaac ttgaatcaaa actgatagag cctccgcaaa ataaattcct ccaattatag
    43261 gtattataag cactgagtta gttagaactg aaaatgctac aactgcacct cctaatgcca
    43321 ttgaacctgt gtcgcccata aacactctag ccggatatga attaaatcct aagaaaccta
    43381 aacatgctcc aacagttgct gctgctaaaa ctgcgacttc tgtatttcca gcaatagaac
    43441 ttgcaaatag cataaagaat acagaaacta ttaatgttac tcctgatgct aatccatcta
    43501 atccatcagt caagtttaca gcatttacta ttgctacgat tataaacatc attattggta
    43561 tatataatat tccaacattt atcacaaaat ctgtaaatgg tatcataagt tgtgaagcac
    43621 ttgatgatga tgtatactga taaaaagcaa cataaaaaga taatgcaact tgaaggatta
    43681 ttttttgata tgcttttagt ccaagtgatc tttttagctt tatttttata aaatcatcta
    43741 aaaagccaat aaaaccaaaa cctgcaatac atataagacc tactgccata tcatgactaa
    43801 cttttactct tgtaagtcca gttattagaa ttgcaacaat cattataatt ccacccatag
    43861 taggtgttcc gtttttagct agatgtgttt ggggtccatc atctctaact gtctgaccaa
    43921 atttaaattt tctaagcatt ggtataaata ttggtcctat tataattact attaaaaacg
    43981 caatcaatgc ggtgtacgta agctctgtta ttcctaacat cataaaactc ctctcccttg
    44041 tcaattaact cattagttta agcttattta tttagatatt cgactatttt ttctaatctc
    44101 atgcctcttg agccttttac taaaatagtg tctcctgttt ttactaattc atttaacttg
    44161 ttaaacacat cttctctatt ctcaaagtga tatatattgg ctgaattgaa tcctaattgt
    44221 tttgcttctt ctccaataaa cactgaattt tcacctatag ttattataat atcagtatta
    44281 ttcatagatg attttccaac taatctatga ccatactctg aaatttcacc catttctagt
    44341 atatctccaa gtatcgctac ccttctacct ttatatcttc caagtatctt taaagcagca
    44401 tccatagaat ctggactagc attatatgaa tcattaatta ttgttaattt ttcatttttt
    44461 attatgtcta gtctcatttt tgtcccttta aaatttttta aacctttttt tatataatct
    44521 aaagaaatat ttagacacaa accaactaat atagcagaca ttgcattata tatattatgt
    44581 tctcctattg ttggtataaa tatttcctct tttttcccat ttattacaca tataaatgtt
    44641 aaactatcct cttccatagt ataactttca caataaatat cattgttttt attaaagcca
    44701 aatgttttta acttatatac atgttctttt tcttttaatg tagataaaaa cttatcatct
    44761 ccatttacaa ttaaagtatt tgtttcatca aagtttgaag taatctccat ctttgctttc
    44821 aaaataccct cttgtgaacc cagtttttct acatgtgata atcctatatt cgatatgact
    44881 gcaattttag gatttactat atccactaaa tattttattt cattgaatcc agacataccc
    44941 atctctatta cagcacactc atgttcttta tttagattaa ataaagtcaa tggaactcca
    45001 aaatgattat ttaagttttt ttcatttttt agtatattta actttgcaga tatggcagag
    45061 taaatcatat ctctagtagt agtttttccc acactacctg taacacctat aaatggaata
    45121 tcaaattttt ctttatagta ttttgaaata tctcctagag ccaaagatgt atcttttacc
    45181 tctattaaat ttatatccga actttctaat tttattccat tactttcatt ttttataaaa
    45241 gttttacatc cactatcata tgcaggcttc ataaatgtat gcccatctaa attttctcca
    45301 actatggcta caaatgcatt ttgggcactt gcctccctgc tatctataac tatatcactt
    45361 acacagtcat tataatctcc atatattaac ttcccattag ttgcaagtac taactctttt
    45421 attgttaagc actccattat taaactcctc ctttaaaacc ttagctatat tctcttagca
    45481 acaaaaggca atgtataccg ttatatatac attgcctacg ataactctta actttataat
    45541 tatatactat atttctgttt ttttgaagat gtttatgggt atatgttaat tatctgttac
    45601 tttttagttt tcaaaatata gggttatact tgagtcttca ttaactttta ctccaggttt
    45661 tgggaatata tcctttacct ttgtatcttc tggaagttct atatctgcat ctaagtctgg
    45721 ttttaacttc gcatcctcca atgcctttac agcatctcct atttttagat ttcttacatc
    45781 tggcacttta acttgcttct tttcatactc agctttttct tcttctttat atactggttt
    45841 aactcccaaa tattttaaag agtcattcat tatttctttc actattggtc ctgctgtagt
    45901 actaccaaat gcacttactc ctgttggttc gtcaacaata gcaagaacta ctatttgcgg
    45961 gtcatcacaa ggtgctattc caacaaatga acatatatac tttcctggag catacttacc
    46021 atctataact ttttgagctg tacctgtttt accaccaagt ctataccctg gtatatatgc
    46081 tatctttcct ccaccttcag ttacaactga ttctgcaatt tctaacattt ttttagatgt
    46141 ttctttagat ataacacttc ttactttttt tggttttact gtttcagtta tatttccttt
    46201 attgtcagta taagatttta ccactcttgg ttgcattaaa tctccaccat tagctattga
    46261 tgatatagct gttataagtt gtattggagt tactgatata gattgaccaa atgatattgt
    46321 agccaattca actggtccta catttttttc gttatacagt attcccttag cttctcctgg
    46381 taaatctata cctgtcttat ccataaggcc aaatgattct atataatcat acatttttcc
    46441 aacacctaat ctgcttccta attcaacaaa tactgggttg caagagtttt gtactgcttg
    46501 tttaaattct tgtgttccat gaggtctgta atgtctccaa catttaatct ttcttcctcc
    46561 aacagttaca ctacctgtac atgtgaattt ttctccatct ttaattactc cctcttcaag
    46621 agcactggaa gatgtaataa gcttaaatgt agaacctggt tcatatgtat cacttactgc
    46681 tggatttctc cacatttgat aatagccttt tattttatct ttatcattat atttttcaag
    46741 ttcttcttga taatatggat atattggcgt tcttgagtca ttagggtcat aatctggttt
    46801 tgatgctaaa gcaagtatat cacctgtttt agggttcata gctattgcag taaccttttt
    46861 tgcattattt aactcataag ctttctgtac tgctttttca gtatagtgtt gaattacttc
    46921 atctatagac aacacaagac cattaccttg tacaggctca taatattttt ccattccttg
    46981 tggtatttct cttcctgagg catcagtact tactataagt tttccaggct ttccttttaa
    47041 ctttttatca tattgcattt ctacaccaga tatacctgtt gcatctgaag aagtatgtcc
    47101 taacacatat ggcgcaaaat tcccatatgg gtaatatctt tgattatctt ctgctaccca
    47161 tattccactt aatttagcat ctcttatttg acttgcctta tcatcatcta tccatctttt
    47221 tacttttaca agagccatat tttttttgct tattaaagcg taaatatctt tataatcttc
    47281 atccaaaatc tcagctacct tttcagctgc ttctttttta tcttctactt ccacaggttt
    47341 acaccaaaca gtatacttag ttacacttac tgctaattct ttcatatttc tatcatatat
    47401 agttcctctt ttgggttcta ttggtatatc tcttgtttgt tgttctagtg cttttgtact
    47461 caaccaattt cccttcataa gttgtaggta cccagtccta ataacaaggc aaaaaaacaa
    47521 tgcacatgct agaataagaa cgagtaccag ccttttctta cttatcctct ttacttttct
    47581 caaatctatt ccccctagtt atccacatct atatacttta tttgtccatc ttttggatag
    47641 tccatattga gcaattcctt agcctttagc tcaatttctt ttattgatgt tttttcagtt
    47701 tcttttgcct ttagttcctc taatactatc tctttttttc ttaaatcttt ttgtaaataa
    47761 tagatatcgt acttcatttc cgagattttt gaataaccac atagattcaa tattattact
    47821 gaaaaaattg caatcagtaa aatgcattta gctcctattt ttttcttatt tctcttttta
    47881 taattattct tttttttatt tttatattca tttagattat gtattttata attttttttc
    47941 aaattctcac cccgatagta tatagtttaa attttcctct atactatatt tgttattaat
    48001 catatttctt cctctaaaat ttcatcgcta aattaataca ctattacaca atctaattgt
    48061 atttttttct atacacaaca aaaacctata aattcactta tacttttata tgaatctata
    48121 ggttagatat taatttctat ttatttttat cacttctagt atttttagat aatttcttcc
    48181 ataaatataa tatgtaacta actatctact ctctgattcc taagttctcc attatattac
    48241 ttaataccat ctatatctat atttttatat ctaaattttc attatatatc tcaataacaa
    48301 agaacatttc ttatagtgtt aattgttttt taattgcttt accagttcaa caaaatatat
    48361 aatacttttt aattaattat agtaatctta ttttatatat aagattattc tttagcctta
    48421 taactactta tattttttct gctactctta gcttagcact tcttgaacgt ggatttactt
    48481 ctatttcttc ctcactaggt aatataggtt ttctagttat tattttcacc tcagattctt
    48541 tatcacattg gcatataggt aaatgttgtg gacatataca atctgatgct aaatgcttaa
    48601 atgcattttt cactattcta tcttctaatg aatggaatgt tattatacat attcttccac
    48661 ctttattcat tatagaagaa gcatcattta tcatctttgt aattactcca agttcattat
    48721 ttacttctat tcgtattgcc tgaaaagttc ttttagctgg gtgaggtcca tctattctag
    48781 ctttcttagg gatagctttt ttaattatat ctactaattt tcctgtagtt tctattggtt
    48841 tgtttgctct ttcttcaact ataaatttag ctatacgctt agcccagtta tcttctccat
    48901 aatcttttat tatcttagtt aattcatctt ctgtataagt atttacaaca tcatatgcag
    48961 aaaactcaca cctaacatcc attctcatat caagtggtgc atcttgcata taagaaaatc
    49021 ctctatcagc ttcatcaagt tgatgagatg aaactcctaa atcagctaat acaccatcaa
    49081 ttttgtaaac tcctattttt tctaattcct cttttatatt ttcaaaattg ctgtgaacaa
    49141 acttcactct actttcatat tcactcaatc tctcttttgc tgtgcttata gcatttttat
    49201 cttggtcaaa ccctataaat aatcccttat ctgacagttt ttttactatt tctttagaat
    49261 gtcctgctcc tcccatagta cagtcaacat acacgccatc tggctttata tttaaattct
    49321 ctatacactc atttaaaaga actgatacat gatgaaattc catagttacc tcccttaaat
    49381 tcacttttta aactattctt cactaatatt atgtttattt ttctttgata aataaacatg
    49441 tgcaattatg ccaccaataa ctccaattag acttataact tgtgccattc taagtggtcc
    49501 taacattaaa ctatctgttc ttaatccctc tataaaaaat cttcctattg aatataaagt
    49561 tatataagta actataactt gaccttcata ttttttattt tttctaaata gtaataaaac
    49621 tataaatatt ccaaaatccc atatggactc atataaaaaa gttggatgta cctttactcc
    49681 atcaaccatt attccccaag gtaaattggt agcacctcca tgtgcttctc cattaataaa
    49741 atttccccat cttccaatag cttgtgctaa aggcattcca agtataactg tatcagccat
    49801 ttttaagaag ttaatatttt taatcttagt gtatatataa ccagctaata ttccaccaat
    49861 taaccctcca tgtatagcaa gtccaccacc tctaaaatta aaaatttgag acatattttg
    49921 agcatagtaa ctccaattaa atacaacata atagattctt gcacagatta gtccaactgg
    49981 aattgctatt atagctatat ttaatacatc atcatcttta attcccactc tttttgcttc
    50041 ttttattgct attaaagttc ctaaaatcat tccacatgcc ataagtattc cataccacat
    50101 gatgtctatg ccaaacagtg taaatgctac tctatccata acgtcacctc tttgtattaa
    50161 ttgaaaatat ttccaaagca atatgctata attacaatta attgtaatta tactaaatta
    50221 tgataacaca ttaatgttaa ttctacaaaa aatattaaaa tttattctac aaatccctac
    50281 actatattat actaatataa ttactataaa attttttaaa ctgaatctct catgactaaa
    50341 gttacaagtg tacatagata ttttttaatc aaaaagattg atgcatatat acatcaacct
    50401 cttctattcc ttatctatag ctcttaaatt tttacctcaa tttatataaa tatattttat
    50461 aaatttgttt taccaaaata aacatttaaa aacataaata atacttaatt agttattctt
    50521 attatgatta agtttctatt taggttctac aatagaaata acacaatact cttctttaaa
    50581 atgctctttt aatctttctt caacttcctc aaaagtaata tctttaataa catctaaata
    50641 atccaataga tttatatcct taaaaacata agatataaaa ctattaccta taaagtttat
    50701 agaatcaaag cacttaataa atgaccctat ttttttcttt ttagttctct caaactcttc
    50761 ttttgataat ccttcttttt ttgatttttc tatataatca agaattattt cttttacttt
    50821 tttaggctct ttagaatctc cagctattat gctaaaagca taatccactt gagacgaaaa
    50881 tccagctcca aaattttcat ttattaaacc ttgcatatac aaatcttcat agagcttact
    50941 tcctttttta aataacatcc ctacaagtat atcagttaca atctctttac gtaaaagttc
    51001 tttccctttt aaaccaacat tactatcttt aaaacctatg ttgaacatag gcatagatat
    51061 agggaatttt tctataacct ctttttcttt tacactttct ggttcttctg gataaaatct
    51121 ttctatactc ttagagagtt tgtctacttt gtaattgttt gactttttag ttacatctat
    51181 aactttttct acatctaagt caccaacaac aaataaagcc atatttcctg gattatagaa
    51241 agtattataa catttatata gctcttcctt agttatctta tatatactat caacagtacc
    51301 tgctatatca attctagcag gataatttac atacattgcc ttcaagcaat taaagtatac
    51361 attccaatca gggtcatcat tgtacatttt tatctcttgt gctattatac ctttttcctt
    51421 ttcaacattt tcatctgtaa aataaggtgt ttgaacatag tcaattagat gttctaaact
    51481 ctcataaaaa ttttcagtag cagaaaataa gtaggctgtc attgtaaaat tagtaaatgc
    51541 atttgcattt actcctaatt ttgaaaattt atcaaatgca tctcctccat caggttgctc
    51601 aaacatctta tgctctagaa aatgtgcaat accttcattc actctaattt tcttatcttc
    51661 tccaattggt acaaattcta agtcatttga accataatta gtagccaaaa tagcatactt
    51721 tttcataaaa ccccttttag gcataaaata tacatctaat ccattttgca atttctcata
    51781 ataaacttct tctttcaata tatcattaac tattttttcc atagacatct cctccattaa
    51841 tttctcaaga agtatacagt atcaagctct atatttttta tagcttcaac tatatctttt
    51901 tttgtaacct tttctatact agatattatg tcttgtactt cagaatttgt ctttgccata
    51961 gactgagaga aaacaaaatc agacatacca cctatattat cagttataga tttcatagaa
    52021 ttaactaaag caagtttact gttttctaat tcttcatcag atatatcacc tgcttttaca
    52081 ctttctactt gtttttttat caattctaca gctttatcat aatctttagt ttctatacca
    52141 gatgatataa acatgatact tttatacttc tctattgaag agaatatata gtaacataaa
    52201 ctttcttttt ctcttacatt aacaaataat ttagagtgtg gaccaccacc taagacattg
    52261 ctcccaacta ctaaagcata atatttatct atgtctgcat aatccacatt tgctctataa
    52321 cccataacta acttaccttg agttatttcc atttgttcat ctataacttt tacctcatcc
    52381 acatttttta taaaatctgc tcttggtata tttataattt cttctctttc aaattttaga
    52441 tttttactta ttatatctac aactttatct tcatcaaaat taccttctac aactatatct
    52501 ataggtgatg ttttaagtat gtttttataa tgttcatata actctcttga agttatacta
    52561 tctatttcat cttcataacc aaattcactt acactatatc tctctccttt acacatagcc
    52621 tcaatacatt tatcttgcgc atattccttt ttatcattta ttattgattg tatcttttct
    52681 cttaaattct ctttttcaat atttaaatat tcttctttaa acccaccatc tacaacaagc
    52741 gtattattta ttacttcatt aaaaaattct actacttctt caaatatact ctcatctaaa
    52801 tacttttcac ttatgttaat tactttaaaa ctcaatactt gacgttctcc acgttttaca
    52861 gcatcagctc ccatagatga accatataaa tcatctaatt tattggatat tgctctagca
    52921 ctaggatatt tagcgcttcc acttgctatt attgatggta ttagagcatt ttttgtagtt
    52981 tctttttcat ccaatagtct ctgaacatat aaacttataa gattcgattt aaacttttct
    53041 gtttttatca aagtaagatt aatattatta cctaaattta ttattttttt agtattaacc
    53101 atatgtacct cctattttta tttaaacatc aattttaaaa ttgaaattta tttatttttt
    53161 aattatatat actcacatat aaaaaccttc tatttaatta tatccttttt attattttct
    53221 aaaagcaata tttttattta aattaaatgc aaatattatt taaatcaata tgctttaaag
    53281 tctagtttac aagcttgata tatatgatgt ataatcaata tgtatgtcta taattaaaac
    53341 aattttgcaa tcctatagat tgattgttta aagttatata aagaatataa aaaagaataa
    53401 gagagaggac gattaatatg aaattaggta tagttggttt gccaaatgta ggtaaaagca
    53461 cactatttaa cgcaataact caagcaggtg cagaatctgc aaattaccct ttttgtacaa
    53521 tagacccaaa tgtgggagtt gtatctgttc cagatgaaag attaaacaaa ctacaagaat
    53581 tatataattc tgaaaaaata gttcctactg ctatagaatt ttgtgatata gcaggtttag
    53641 ttagaggtgc ttctaaagga gaaggtttag gaaataaatt cttatcacat ataagagaag
    53701 ttgatgcaat agttcatgtt gttagatgtt ttgaggatga aaatgttgta catgtagatg
    53761 gctctgtaga cccacttcgc gatatagaaa ctataaactt agaattaatt ttctcagata
    53821 tagagattct tgaaagaaga attaataaaa ctcaaaaagc tgctaaggct gataaaactt
    53881 taggttctga acttgattta ttaaaatcaa taatgtctac tttagaagaa agtaagtgtg
    53941 taagaactat ggaatttaca gaagatgaac aaacattcgt aaattcattg gatttactta
    54001 catctaagcc tgtaatatat gcttcaaatg tatcagaaga tgatttagct gataatggag
    54061 aaaataataa atatgttcaa caagttaaag catttgcaga aacagaagat gccgaagttg
    54121 ttgtagtatg tgcacaaata gaagcagaaa tttctgaact tgattctgct gaagaaaaga
    54181 aagagttttt agaaacatta ggtcttgaac aatcagggct tgataagctt ataaaatctt
    54241 cttatgcttt acttggttta atttcattct taactgctgg tccaaaagaa gttagagcat
    54301 ggactataaa agttggaagt aaagctcctc aggcaggagg aaaaatacat tctgatatag
    54361 aaagaggatt tatcagagca gaaacaatag cctttaatga tttggttgag catggtacta
    54421 tggctgctgc taaagaaaaa gggttggtta aattagaagg taaagaatac atagttaaag
    54481 atggagatgt aatattattt agatttaatg tttaaaatga ttaatcatat acactttgac
    54541 tattatatat aataactaaa aaactaaga
//
LOCUS       DAMKPG010000017        53427 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMKPG010000017 DAMKPG010000000
VERSION     DAMKPG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.019"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(119..280)
                     /locus_tag="PC621_001081"
     CDS             complement(119..280)
                     /locus_tag="PC621_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108253.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFD"
     gene            complement(454..1320)
                     /locus_tag="PC621_001082"
     CDS             complement(454..1320)
                     /locus_tag="PC621_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4108254.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1546..2415)
                     /locus_tag="PC621_001083"
     CDS             complement(1546..2415)
                     /locus_tag="PC621_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4108255.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2921..3142)
                     /locus_tag="PC621_001084"
     CDS             complement(2921..3142)
                     /locus_tag="PC621_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108256.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3643..4623)
                     /locus_tag="PC621_001085"
     CDS             complement(3643..4623)
                     /locus_tag="PC621_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108257.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4787..5659)
                     /locus_tag="PC621_001086"
     CDS             complement(4787..5659)
                     /locus_tag="PC621_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4108258.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(5823..6374)
                     /locus_tag="PC621_001087"
     CDS             complement(5823..6374)
                     /locus_tag="PC621_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108259.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6394..7296)
                     /locus_tag="PC621_001088"
     CDS             complement(6394..7296)
                     /locus_tag="PC621_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF4108260.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7311..9683)
                     /locus_tag="PC621_001089"
     CDS             complement(7311..9683)
                     /locus_tag="PC621_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF4108261.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(9902..10591)
                     /locus_tag="PC621_001090"
     CDS             complement(9902..10591)
                     /locus_tag="PC621_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108262.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(10832..12211)
                     /locus_tag="PC621_001091"
     CDS             complement(10832..12211)
                     /locus_tag="PC621_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF4108263.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     EQDTDSWGD"
     gene            complement(12205..12918)
                     /gene="rgbR"
                     /locus_tag="PC621_001092"
     CDS             complement(12205..12918)
                     /gene="rgbR"
                     /locus_tag="PC621_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF4108264.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(13169..13729)
                     /locus_tag="PC621_001093"
     CDS             complement(13169..13729)
                     /locus_tag="PC621_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF4108265.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(13825..14628)
                     /gene="zupT"
                     /locus_tag="PC621_001094"
     CDS             complement(13825..14628)
                     /gene="zupT"
                     /locus_tag="PC621_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF4108266.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(14779..15942)
                     /locus_tag="PC621_001095"
     CDS             complement(14779..15942)
                     /locus_tag="PC621_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108267.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(16002..17189)
                     /locus_tag="PC621_001096"
     CDS             complement(16002..17189)
                     /locus_tag="PC621_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108268.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(17552..19252)
                     /locus_tag="PC621_001097"
     CDS             complement(17552..19252)
                     /locus_tag="PC621_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108269.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(19508..19783)
                     /locus_tag="PC621_001098"
     CDS             complement(19508..19783)
                     /locus_tag="PC621_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108270.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(19902..20453)
                     /gene="mobB"
                     /locus_tag="PC621_001099"
     CDS             complement(19902..20453)
                     /gene="mobB"
                     /locus_tag="PC621_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF4108271.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            complement(20458..21639)
                     /locus_tag="PC621_001100"
     CDS             complement(20458..21639)
                     /locus_tag="PC621_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF4108272.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(21850..22584)
                     /locus_tag="PC621_001101"
     CDS             complement(21850..22584)
                     /locus_tag="PC621_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108273.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            22946..23851
                     /locus_tag="PC621_001102"
     CDS             22946..23851
                     /locus_tag="PC621_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4108274.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(24011..24853)
                     /locus_tag="PC621_001103"
     CDS             complement(24011..24853)
                     /locus_tag="PC621_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF4108275.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(24837..25595)
                     /locus_tag="PC621_001104"
     CDS             complement(24837..25595)
                     /locus_tag="PC621_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4108276.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(25612..26082)
                     /locus_tag="PC621_001105"
     CDS             complement(25612..26082)
                     /locus_tag="PC621_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108277.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(26082..26825)
                     /locus_tag="PC621_001106"
     CDS             complement(26082..26825)
                     /locus_tag="PC621_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF4108278.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            complement(26829..27242)
                     /locus_tag="PC621_001107"
     CDS             complement(26829..27242)
                     /locus_tag="PC621_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF4108279.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(27268..28020)
                     /locus_tag="PC621_001108"
     CDS             complement(27268..28020)
                     /locus_tag="PC621_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4108280.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(28260..29654)
                     /locus_tag="PC621_001109"
     CDS             complement(28260..29654)
                     /locus_tag="PC621_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF4108281.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(29674..29883)
                     /locus_tag="PC621_001110"
     CDS             complement(29674..29883)
                     /locus_tag="PC621_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF4108282.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(29912..31357)
                     /locus_tag="PC621_001111"
     CDS             complement(29912..31357)
                     /locus_tag="PC621_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF4108283.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            31467..32261
                     /locus_tag="PC621_001112"
     CDS             31467..32261
                     /locus_tag="PC621_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF4108284.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            32312..33634
                     /locus_tag="PC621_001113"
     CDS             32312..33634
                     /locus_tag="PC621_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF4108285.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(33735..34952)
                     /gene="cdeC"
                     /locus_tag="PC621_001114"
     CDS             complement(33735..34952)
                     /gene="cdeC"
                     /locus_tag="PC621_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF4108286.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(35135..35315)
                     /gene="ssrS"
                     /locus_tag="PC621_001115"
     ncRNA           complement(35135..35315)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC621_001115"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(35430..36335)
                     /locus_tag="PC621_001116"
     CDS             complement(35430..36335)
                     /locus_tag="PC621_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108287.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            36547..36945
                     /locus_tag="PC621_001117"
     CDS             36547..36945
                     /locus_tag="PC621_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108288.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            complement(37068..38084)
                     /gene="ccpA"
                     /locus_tag="PC621_001118"
     CDS             complement(37068..38084)
                     /gene="ccpA"
                     /locus_tag="PC621_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF4108289.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(38337..38537)
                     /locus_tag="PC621_001119"
     CDS             complement(38337..38537)
                     /locus_tag="PC621_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108290.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(38571..38750)
                     /locus_tag="PC621_001120"
     CDS             complement(38571..38750)
                     /locus_tag="PC621_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108291.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(38932..39138)
                     /locus_tag="PC621_001121"
     CDS             complement(38932..39138)
                     /locus_tag="PC621_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108292.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(39186..39611)
                     /locus_tag="PC621_001122"
     CDS             complement(39186..39611)
                     /locus_tag="PC621_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108293.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            39783..40010
                     /gene="acpP"
                     /locus_tag="PC621_001123"
     CDS             39783..40010
                     /gene="acpP"
                     /locus_tag="PC621_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF4108294.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(40154..41158)
                     /locus_tag="PC621_001124"
     CDS             complement(40154..41158)
                     /locus_tag="PC621_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF4108295.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(41172..42074)
                     /locus_tag="PC621_001125"
     CDS             complement(41172..42074)
                     /locus_tag="PC621_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF4108296.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(42269..43444)
                     /locus_tag="PC621_001126"
     CDS             complement(42269..43444)
                     /locus_tag="PC621_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF4108297.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(43531..44376)
                     /locus_tag="PC621_001127"
     CDS             complement(43531..44376)
                     /locus_tag="PC621_001127"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF4108298.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(44474..45271)
                     /locus_tag="PC621_001128"
     CDS             complement(44474..45271)
                     /locus_tag="PC621_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF4108299.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(45290..46300)
                     /locus_tag="PC621_001129"
     CDS             complement(45290..46300)
                     /locus_tag="PC621_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF4108300.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(46319..47101)
                     /locus_tag="PC621_001130"
     CDS             complement(46319..47101)
                     /locus_tag="PC621_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF4108301.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(47160..48296)
                     /locus_tag="PC621_001131"
     CDS             complement(47160..48296)
                     /locus_tag="PC621_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF4108302.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(49004..49471)
                     /locus_tag="PC621_001132"
     CDS             complement(49004..49471)
                     /locus_tag="PC621_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF4108303.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            complement(49550..50092)
                     /locus_tag="PC621_001133"
     CDS             complement(49550..50092)
                     /locus_tag="PC621_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108304.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(50144..50830)
                     /locus_tag="PC621_001134"
     CDS             complement(50144..50830)
                     /locus_tag="PC621_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF4108305.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(50818..51690)
                     /locus_tag="PC621_001135"
     CDS             complement(50818..51690)
                     /locus_tag="PC621_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108306.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            51825..52625
                     /locus_tag="PC621_001136"
     CDS             51825..52625
                     /locus_tag="PC621_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF4108307.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            52650..53219
                     /gene="yfcE"
                     /locus_tag="PC621_001137"
     CDS             52650..53219
                     /gene="yfcE"
                     /locus_tag="PC621_001137"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF4108308.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT        17350 a         9441 c         5598 g        21038 t
ORIGIN      
        1 tagcaattca agtagtattg gataggttct atcctatctc tacatataag tttccctata
       61 tgctgactat tttagctact aactcatgac taaagtcaca agtgtgcgta gctatttttt
      121 aatcaaagta tctagctatc ttaaaggcta agatgataga aggattatac ctaccatttt
      181 ctaatgaacc tatagtttgt cttgatactt gcaaaataga agcaagttct tcttgtttta
      241 tgcctctttc ttttcttatt tcttctaatc tatttttcac atctatcccc ctttaatatc
      301 tcaactacgg aaagtctact ttccataatt atacaactat atttcattta tgtaaagctt
      361 gctttccata tttacaaaaa aataacagac tattttcagc ctgtaatttt aaaatttcat
      421 gttgtttatt aaatttaatg ttattcatta actttaactt ggtttctgaa ttggaatcca
      481 aacttctgta gtataatcag gagcattgat atcgccattg ggataaagtt caatatctgg
      541 taagttagca tacacatacc cagaacttgg taaccactca gtaataattc ttttttgcaa
      601 ttcctgtatt gccagagcat ttggcattgg agaaacacat tcaaatactg cccatgtacc
      661 tgatggtatt aagtattcac gagtatcttc taatacaggc ttatctgttg gtgcagcaat
      721 ataataatct aaacccttac tatttgtaca cacactaact ccaagcaatc cacaaggttc
      781 gttatttatt aactctgaaa gtttatcaat tgttccattt tttatgcatt tagcccataa
      841 cttaggaact ttctcaaagc actcttctac aatcatgttc atactttctt ttataccaac
      901 aatacgaaac cctttctttt ctacaatacg atattgcatt tctacatttc cttttacata
      961 aatgcttaat gtcatcttag gatatgcttt taaaacaaca ccctttgctc tagctgcaga
     1021 tggagaaatg ttatgtatat tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc
     1081 atacttctca gatagttcta aaattttgat gttactgttc tgaagttcaa atgcagcctt
     1141 agtcatacgt cttcgtctaa tatattcaga taatggtact tctgcaatat atgaaaacat
     1201 tctttgaaaa tgaaaggttg aacaacatgc aatttttgct gcttctgcat aatccactcc
     1261 actttcgaga ttttcttcaa tataagtaat agcttgattt aatttattta tccattccat
     1321 ttgatattac ctccaagcaa attatacagt ttaataaaat aatatacctc tctattcatg
     1381 cacaaaattg taagcattta aattcataag tatttttcaa tcaatacaat aaatataaaa
     1441 attgtattat acaataaaaa agttcaataa cattttatta taaaatatta ttgaactttt
     1501 tataaaaatt aatttaagtg tagttaaatt aagtattaaa ctatcttata tattacaata
     1561 tcctactatc ttatagtctt ctggcacttt ataatctttg tctggagtac ctaaattcca
     1621 ttttaaatca aacatagttg cacttattat tgttgcaaaa taaagcataa ctataccaat
     1681 gtctattttt tcttcatata aattagtatg accatctttt ctaacagcta aaactacagt
     1741 gcctccatca actataaatc tccatggttg tctatttaat gtagatggag ctaatcttgc
     1801 atagtgaaat gcatcaagaa gtgctctttc ttctaagatt tctatacttg caacatttcc
     1861 ccattcgttc atataaacaa catcttctac acctagtcta gtagacccct tatctgttac
     1921 aacttgcata tcagattttg aataattttg cccagttttt gtagcattta atactttagc
     1981 attacttaca ttttctccat atccaatagc tataattcct gttacctctt tatctgaatt
     2041 aattccaagt ttttctttta ccttttttcc atcttcaaaa gtaacccaac aagaaccaat
     2101 tccaagttct gttactttaa agattatact ttctcctatg taccctgaat tttcaatgta
     2161 attatctttt atgtcagata atattataat ataacttgga gaatctatca tatttccttt
     2221 gtaccctgct actttatcta aattattaaa aacttcactt ttattcataa ttcttacatc
     2281 aacatctatt tctggaacta gtttcttaca agattctgag tatttcttaa tttcactaag
     2341 atattcttct ttaatatcta tgttcttata ttctctaaca gactttctat ttaatatgat
     2401 tgttttataa tccataattt tatcctcccc aaactagtat tgtcttaatt atatacccat
     2461 taatatatta ttttactcat ttatttaata aataaagatt acaattttgt aattattatt
     2521 tattaataat attccacttt tacattaata atgaccagtt gtgcaaaatc cattgccaac
     2581 caccaaataa aaacatatta taaaagtaat ataaaataac aaacaaatct taacaaaaaa
     2641 ttaaactttt tttacacgaa agaaggcttc ccgttgcctt ctttattttt ataaaaaaat
     2701 aagctgtttc taaaatgatt tcacattttt gaaacagcca agttattcta taatgacaac
     2761 tataatttga ggatgtgagg gagagttgtc attataaatt tagggtaatt atattatgtt
     2821 ttaagctagt tcttcctata tttatataat acattttata tatgaatttt atatgagatt
     2881 ggtataaatg atatatgaaa ttacatatgt ttatattata ttaaatttta ttattacatt
     2941 ttccacaaca atactcatat gtctccacat tatttggaat atttacaaat acaaattctt
     3001 tacagttctc acaatacttt aatatagata tagttccaca agtgtaacat tcaaatatta
     3061 cacaatcctc attttcctct actatttcaa actttttact atgttcacca cactctgggc
     3121 acttgtgatt atttacattc ataaatactc ctccttatta gtatagttac atttcaaaaa
     3181 cttaattaat gtattctata ttacttatta tatattctat aataaatcat attctaatat
     3241 aataaaacaa attaaataaa aaaaaaccat aaattttaaa tttatggatt aattcttgta
     3301 atgataactc tatttgagga tgtgagggag ggttatcatt acaatagggt attatattat
     3361 ggtttttaag ctagtctttc tacacctcat atgatacata tgctatatga attttatatg
     3421 atttttacat aaatatttta tgaaatttaa attttttagt cattttgaaa tttgtaaaaa
     3481 gttctctaaa aatccacagc atcataaaca gccaaaattt aagtaaaaca tcataaaaaa
     3541 atttgtccaa taattttaac aagtcatttc acctcattat aattattgac acaaacttaa
     3601 ataaacaatc tataaatctt atataaatct taaactttac ccttattgat acacaatttc
     3661 tagtttgtca attttatttt gcatctctaa gaaaatttct tcaacagaat tgttaaatat
     3721 attgcctaaa tagactcttt tatctttttg aaatgaataa gattttacac taccatcaga
     3781 agaaatatat aaatgactcc actctgctcc atattttgaa aacctctcct ttgtatcttt
     3841 aaacctcata tcttctattt ttattgcttt ttctctgtct cctcttacta cagtgtgacc
     3901 cctttttatt aaactggtta tttttatatc ttcttttaat ttaatacgtg tatcatcatt
     3961 ttttgaatat actttccata tcactttatg acgataatta tctaattctt ttattaagct
     4021 tctatcaata gtaaagctat ctttagttac cacaattaaa tcaaaatggt ctaacatata
     4081 ttgaaatatt tctctcatac ttttattttt taaaatatcc tcaccaataa cctctataaa
     4141 tctacagcct ttctcataca tgcagttcaa taaacttgtg atttcagaaa aataatcatt
     4201 tgtattatta tctccatcta tttctatagt aaagtacaaa ggagtaatgt atgatttatt
     4261 tcctgttatc tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg ttaagtatac
     4321 attctcataa aacataaaaa attccataac tttttctttc atattatgga tatttctata
     4381 tctattggat aatatattta ttatatcgtc agtagtattt ttcccatcac attcagccag
     4441 tataacaatt tgcttccaac ttaagctatc cacatttccg atactttttt tatatccatc
     4501 ttcttcatca taaataggac caattataat gttttctttt ggccacagat gaaccctagt
     4561 tccatctttt aatttcggat atttattttt tacagactca taaaatctac ttgtatcacc
     4621 catgattctt tactcccccc catagtttaa atattctatg ctaaaaatag cactttattt
     4681 ttattaattc aacaattatt caaaattggt cattatatat gtttaaatgg atttaacatc
     4741 aaccttattt atatgttatt gatgtttata tatgttatat aaaatcttat ttctcatact
     4801 tcaatgtaat cttaactatc tctggtttac aattaagcct aattaaaact tttgagcctc
     4861 ctaagccttt atttaaaatc atagtgctat tatctttttc atattttccc cagtcatact
     4921 taggaaagaa ctctctattt ggagataata cacctccaac aaaaggtatt cttattatac
     4981 ctccatgaac atgtcctgct aaaaccaaat ctgctcccca ttttgcatat ttttcaaaga
     5041 aaaatggtgt atgtgctaaa agaatgttat attcattttc attaacttta cctagattgt
     5101 cttctacaaa attgtcactt agttctaatt ttttgttatt agcaaataaa tatggatagc
     5161 attccaatgg tataattaaa ccatataaat taagatggct attacctcta tatattctta
     5221 cagattcatt ttccatattt attattttct ttgaatatag ttccttaaaa taatccttat
     5281 atagatgttt atactttttt attaaagatt tttgttcatg atttccaatt atatgatata
     5341 cttcatattt ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa tttttatttt
     5401 ctccatctac taaatctcct gttataaata taacatctgg gtttaatgat tcaattttat
     5461 ctatcaatac cttattattt ttatcaaaca ccttattatg taaatcactc acttgaacta
     5521 ttttaaagtt atcaaattcc tttggaattt ttttgttgaa aatagtatat tctgtcgttt
     5581 ctatatgctg agtttcatac ttatacagaa tatacaataa tattaatact aatactgtca
     5641 ttgaaacctt tataatcata ttctcttcct ctttattcta ttttgttaat attattataa
     5701 tgcttttatg ttttttttaa aacaataaca tcatcacata catataaatc ttttcgacat
     5761 tataaaaata agttatcctt ttctaaaatt ttcatttatt ttagaaaagg ataactataa
     5821 aattatctat aaacctctat attgtgaaat atagtttttc ctttatttaa atttttatat
     5881 atatgctcta aagttccatc aaacctaata gaatgtccag actctaaaat ataagttttc
     5941 ccatccacat taaggtcaag tttaccttct attacgagta catactcttc taaaacataa
     6001 ctatgtgtag atgaaacatg agtacagttt tcctcaagct caattataaa aagttcaaac
     6061 cttctttgag ggctaaaagg gtatattgga tataacttca tgttcccttt ttgctcaaca
     6121 ataggctcta cttcttcttt ctttataacc tcataggtat ttgtattttc atttaatagt
     6181 gtagtaaaat ttattttaaa tccagatgaa attttccata aagtagatac tgtcggactt
     6241 gattcacctc tctcaatctg tccaagcatt gctttactga cacctgtcat ctttgaagct
     6301 ttgtctaaac ttatgcccat ttcttgtctc aataacctta aattttctcc cacattagga
     6361 aatttattat ccatgttaaa atcctcctga tagctatttg tattctatga tttttactcc
     6421 tgtaagttct actatctctt ttaattttaa catatgattg ttacaaattt catcaaattc
     6481 atatatataa tcttcaagcc ctaatgcctt atatttatcg attccaaatt tatgatatgg
     6541 aagtaactcc atcttgcctc ctggaacatt ttgtttgaca aataatgcag tatttcttat
     6601 attttcttca ctatcattta ctccataaat aattggcact cttataacca tagatttatt
     6661 caactttgaa agcctacata tattatctaa tataatttta ttacttactc ctgtatattc
     6721 tttatgaata ttatcatcca tagattttat atctacaaat atgtggtcta ttttctcaaa
     6781 tacatcattt accttattcc aatcaaaata tccacaagtt tcaattgcaa tatttatgcc
     6841 tttatcataa aaaatatcaa ctaattctct taaaaaatca atctgaagtg taggttcacc
     6901 tccagaaaaa gttatccctc cattagattc aaagaaaaat ataaaatcct tttcaacttc
     6961 ctctactatt tcttcaacac tcattttttc ggtcattata caaatggcat ctttaagaca
     7021 taccttgata cactctccac ataaatcaca tttattattt atttgcgttc tatcaaatag
     7081 tgaacttata ttttgaggac aaacatctat acatctatta caggatacac atttatcttt
     7141 cataacacct aattttacta tgttactcca ggaatctgga ttagaacacc acttacattt
     7201 taatttacat ccttctaaaa atatagtagt ccttattcca tctccatcat ttactgaaaa
     7261 atgttgtact tttacaactc tacctttttt actcataatc tacctcataa ttaaaaattg
     7321 ctatgtgaat ttctagctat tatagcatct tgcatttctc ttgataaatt aacaaattga
     7381 gtactatatc cagcaactct cactaataaa tctttatatt cttctggatt ttcttgagct
     7441 tttaatagag tatcatttga tatcgtatta aattgtacat ggaatcctcc taaagcaaaa
     7501 tatgatttaa tcatagatgt tagatttcta agtcctcttt cagtttccac caaatcttga
     7561 ttcagtctta aatttagaag tgttccacca gtatgaacat catgatttat ttttgatgca
     7621 gatttcatgg ctgctagtgg acttgttgta tcagtaccaa caaacggaga aactccctct
     7681 gtcaaaggtt ttgtcgcttt tcttccacaa ggagttgcac ctacgatttt tccagttggt
     7741 acatagtttg atatacccat aaatgcacta ttaaatttag agccaaaaat atccttgtat
     7801 tttctagttt cagtataata aaaatcagaa acttcaattg ctaatgaatc tacataatca
     7861 ttatcatttc catacttagg aacatccaac gcaagttttc ttaattcttc ataaccttcc
     7921 caatcattat ttagtgcttt tgttaattct tctagcgtag tcaccttatc ctcaaatact
     7981 aattttttta tagctgccaa tgaatttgaa actacaccta accctatacc agttaataca
     8041 ggacctatat tatattttgc tcctccttta cttaaatcaa ctcccttatc caaacatcca
     8101 tctacacaag tagataaaaa tggtcttggt accatttctt tatgaatttg ttgagcaaca
     8161 gtagttccta taacagaact ttttacaaga tatgctaatt gctttaaaaa ctcatcctta
     8221 agttcttcaa aagaagtaaa ttctgtgata ttttttgtat ctaaccccat tttttctcca
     8281 gttaatctac tcttaccttc attcatagca tattctaaag ctgctgcaaa attgatattt
     8341 acggctgaag tccactgtcc tgtttttcta aaatgaggaa ctacacaacc acagttactc
     8401 caatctctag catcttctgc atcatatcca tcttgtaata acatttgtgc ccctgcacta
     8461 tcgctgtgta tagcagggaa accagttcct tgttttacca atttagcaac tgccattacg
     8521 aaattatctg gtgctccttg actcactcta acacttaatc ctggctggtg agtttttaca
     8581 ctttctgtag cctttaaaca catatatgag atatcattcg ttccatcagt accatttctc
     8641 tttttaccac caacagtaag attttggaat tgattatatc ctgcaaagta atttgctgta
     8701 tttgctgata tagtccatac ccattctgat aacttaatcc aaagtgcttc tatcaattct
     8761 tgagcttctg attcagttat tttaccttct ctagcatcat tttcataata tggatacata
     8821 tattggtcaa atctacctaa atttaatgcc aatggatttt ctgatagaat accacctaat
     8881 tgaacaaacc atacaaattg tattgcttca taaaaattag ttggagggtt tgctggtaca
     8941 ttcatacata tctctgaaat ttttataagt tctaactttc tcttttcatc tgtttcttgc
     9001 ttactcatct cttttgcaag atttgaatat ctttgtgcca atgtcataat accctctgca
     9061 actatagaaa cagatttgta aaaatctatc ttttcaatat tttctgaaac tgatatatct
     9121 aattctttaa tttttttatc tgcatcttcc ttaatccctt tatatccttt tacaaacaat
     9181 atatcttgat aatctggtgt tacttcacca actgcctgtc tccacttaga atcattatct
     9241 attattcctg tatctactaa tatcttagct gtatcttctg ggattcttgc taagaaaact
     9301 tcttccaaag attttccctt ccaatatgga aatatttctt ttcttataaa ttctatttgt
     9361 tcttttgata ttacatatgg gtcttgagtt cttttatcaa aagtatccat ttctttgtct
     9421 acccattgcc aagaaaactc tggtgtcaat atccctgttc ttctaaattt tcctgctgta
     9481 cctactatta attcattttc aaagattttt ataggaagtt ctttacatgt ctccaaaaaa
     9541 gctttagctc ttcttataca gatagcttct ccctcagttt ttttatgtga ttctgtaaaa
     9601 attctagccc tttcacaaca tatcataggt ttagaattaa tatattcttg tctaaatgct
     9661 tctattctat tagtttgact catttaaaat ccccctttgg ataatatata ctacattttt
     9721 gtatgttata acatacttta attatattat actgtccttt agcataaaat ggaagcaaaa
     9781 taactttttt tttcatctac cttgaacacc tatatattaa tacttttttt gttaaattta
     9841 taaaatctaa aaaaattttc tatgatatat tttctatatt gttatagtta tttctaatat
     9901 actaatgatt tgaaatatca aatttgtata ctagacttag ttttaaaacc ttagttgatt
     9961 gtttaacttt gttaacttca acatgtatat ccttcatata aggaagctcc acaacaccta
    10021 ctcccttatt aaattcttct tcaaccatag aaaaaatttc tttctcagtt aagttcgtat
    10081 tagtcaatga tttaaataaa ttgtatataa atttaatata tttatcactc tcagataact
    10141 tatcatcttc tggatactct ttagcacaga gaatatctat tgttactttt ccttctttat
    10201 cgtcttcaga catagaatag aataaacttc gaatattttt ttcaggtggt ttttcagcac
    10261 catttataga tactacaaag ctaccatctg attttgagtc agtaatacta taaccactgt
    10321 ctattatagc tttcgaaaat ctttgagata aatctttttt tacttcttct ctacctttcg
    10381 catcattaat cttttgtgag taattattaa atagctcgat agattcatct tttttatctt
    10441 caacttgttc tttcaatgaa tctttactaa tttgttctga gctacttaag ttcttatcag
    10501 atacaggaga catacatcct gccatcaatg ctaagctaag atttaaagcc acaattattg
    10561 ccactctatt tatattcctt tttactttca tacacaatcc tcccatcaca ctaaccgctc
    10621 taaagtatta ttcattgcat tagaatatat ttatattttc tttatattaa agatttatta
    10681 ttcaatacat atcaccttcc tatccaattc ttataactta ctattagtaa aattataagc
    10741 tccttaatac agaaataagg atgttatctt ttgttattaa aagataacat ccttattttt
    10801 ttgtatcaat acatatgtcc caactggtaa cttaatcgcc ccaactgtca gtatcttgct
    10861 ccaatggtat atatatattt agtataaatt tatcgatact attttcaaat aataattctc
    10921 cattatattt ttgtaaagaa gatttaatac tctgaatacc tattccatgt acgaacttat
    10981 ccattttgtt tgttaacaat atatttttat taaaagtaag ctgatttagt ttactatttt
    11041 cacatttaag tacaaaaaat gactttgtta tagttccttt aattctaata tatttattaa
    11101 tattttcatc cactatttta tcacatgctt ctattgcatt atccaaaata ttagaaaata
    11161 tgcttgacac atctataggt tttacaaaat ttacttttga aaaatttata gagcaagtaa
    11221 atttaattcc tttttttaaa catatttcac tcttttcgtt gattataatg tctaatatca
    11281 tatttcctgt attatagata tatttaaatg tttttatttc atcttttaaa ttgttaacat
    11341 attcattaat ttctttgcta ttgtttctaa ggttgtctat acaataaatg tggttattta
    11401 tatcgtgata tagtttctta actcgcatat gagattcttg aatacttaaa taatgcgcat
    11461 attgcatatc taacttattt tttattaatt cagcttgaga ttttaatcta ttctctttta
    11521 ttatttcttt aaacaataat ataagaaata tactactagc tcctaccaac tttggattaa
    11581 caaaattatc aaaatagtat ccttctgaat gtaatgaccc aaaccgttct aattttttta
    11641 atacataatt tacaagcata ttcattgtat tagctaataa ggcaaataat aaatatacat
    11701 aatttctctt atcttggcta attaatttta tagacttcct aaaaaagcta aatacaagat
    11761 atataagaat aattattaaa ttactatata tctgaatttt tatagattct aagtgaggta
    11821 ctaataagga ttctttagat gaaataacat tacctgtaat tgcaaaaaat agtattctat
    11881 atataacagc atccaaacat ttataatata cactaaatag ataaaagaaa atggaatact
    11941 taatataatc catcttataa aaataattat agtatcctcc ccaagcaata aatgctaata
    12001 tataaaataa atagtttata ttactaaagt aacttcctcc attaaataat ctacttatct
    12061 gaattataat ataaataaga aacaagacaa tatagaatac tattttttca ttttttttac
    12121 tatccttttt ataaattaaa attccgttca ttatagaaaa ctgtataaat atagataaaa
    12181 ctactaatag ctcattcata aaatttagca tattgtatca ccaagaaatt ttaaaaattt
    12241 ctccttaact tctttatatc tatatctact aataggtacc tcttctccac tttccagtat
    12301 tgcagcatta ggctttatat tttcaacata tcttaaattt actataaaac ttttatggca
    12361 tcttataaat ttatatggat ttaattcttt ttctatttta cctaaactat attttatatc
    12421 aaaattcttg tttattgtgt gtattagcat atcttttttt tgaacttcta tatattttat
    12481 ttcatttaaa taaatcttat atgtattaga cttatttttt attgtaatat gactttcctt
    12541 atttatttca atatctttga tacaagttag cacatgtttt tttagttctt ccaactctat
    12601 tggctttagt aaatacctat aagctcttac ctcataacct tcttgcacat aatctattaa
    12661 tgaagttaca aatattattt ccatttcatc gtctacttct ctaatctttc ttgcagtatc
    12721 cattccattt aatttttcca tttttatatc aagaagtaat atatctatat cttttaaatt
    12781 actttccagt aactcttctc cagatatata cttttgtatc tcatattcta tacttatatc
    12841 ttttaatata ttctcaaggt aatcttctat taaaagttgt gtttcttttt catcttcaca
    12901 aactgctatc ttaatcataa aatgcctcct tacataattt ctagttaata caattaaaat
    12961 atttataata tatttaaaga taattttgta tatctgatac tttaggataa catttttaaa
    13021 ttatttttat caatactcaa tccaatacaa ctattatttg atatttcaat ttaaagacag
    13081 aaattactat aattaaaaac agtactttac taacaaaaga agatgtctta aaatattaaa
    13141 aaaataattt taggacacct tcttttattt atttaaactt tttaagcctt aaataataca
    13201 ttaatctcct atataattgt actgcatata aaatcaacaa tataatctgt agtacaatag
    13261 cagttacaga tgctccataa ccactatata aatttcttat ttcaatattc aataaatata
    13321 taaatattac tactaacata aagaatataa acatattcat aaataaaatt gactttactt
    13381 tgtttttttt atcagaatca ctatataatt taggaggtat gtctttgtat tctttcgacc
    13441 atatatacca tgcagatgta cccataaaac taccaatact cctaaataca agattccagc
    13501 catttgattt atgttcatca aaataattat tctctacatc atagtagtaa tcaacagtgt
    13561 attttatttt tcttggactt cccttatgaa aataaaatat tcctccaaat ttatttatct
    13621 tatataaatt atatccttta gcttcaatat cttctaacca tttctctatc ttatcaggtg
    13681 aactatacca gtcaaatttg aattttattt tcttttccat agcactcatt ctccaaaatg
    13741 atatttttaa atagcttaaa aatctattta aataattata tcattttgat actaatttta
    13801 ggttacaatc atatatttaa aaagctattt aaatagtaat aaactaatag ccataacacc
    13861 cattcctgca atcaatccat aaatagataa atgatgttcc ccgtactctc tagctgatgg
    13921 caataattca tctagtgata tgaataccat aattcctcct acaattgcaa atacaattcc
    13981 aagagttata tcatttaaaa aatttctaag aagtacatat ccaataatcg ctcctaaagg
    14041 ctcagacata ccagataaaa aggaataata aaaagctttc tttttatctc ctgtggcata
    14101 ataaactggt acagatacag atataccttc tggtatattg tgtattgcaa ttgcaattgc
    14161 tatcgggata gctattgtaa catcatcaag agctgatata aatgtagcta atccttcagg
    14221 aaaattgtga attgctatag ccattgctgt aaatatacct gttctcaaaa gagacttatt
    14281 ttttttgata ttcttttctt ctatattctc cattttcttt atttcatgtg gattttcttc
    14341 ttgtggaatt aacttatcaa taacagcaat gatagccata cctataaaaa aagctattac
    14401 agttgaccaa cttcctaatt tctctcccat agattttgtc aaagcatctt gtgctttagg
    14461 aaaaatttct atcatagaaa cataaatcat aacaccagca gaaaatccta agcttataga
    14521 aagaaactta gtatttgttt ttttagcaaa aaatgctata caacttccta taccagtaga
    14581 taaaccagcc aataatgtta tcaaaaatgc aaatattaca ttattcaaat aaatcacctt
    14641 ctttttaatt gatattgatt atcattttct ttatgctatc atatatattt tatatagtca
    14701 atatttataa aaaatataca caataaaaaa gcagagatat aaacttagtc tctgcttttt
    14761 ctttatagta tattaaaatt atttatttaa gaaatccact gcatattgca catataaagc
    14821 agttccaatt tctagagcat cttcgtctat attaaagcat ccatgatgat gaggatagca
    14881 agcacctttt gactcatttc tagctcctac aaatgctagt gcaccaggtg ctatattcat
    14941 aaactcagct aaatcttctg ctcctgttac tttttcaaat agtgttatac agtcttctcc
    15001 aaatagctta attgctgcat ctgtagctat ctttgaacat tctttgtcat ttataactgc
    15061 tggtgttaag tacccatact ctaattcagc atctgctcta tatgcttctg ctgtagattt
    15121 tgctatacgt tctaaaattc caggaatttg ctttctaagc tctggattaa atagccttat
    15181 tgtaccttct aagacagctt cactcgcaat tacgttaaat ctagttccag aatttaaaac
    15241 tccaacactc acaactaatg gttccagagg acttacttct ctactaacca tagattgtag
    15301 attcataact attgctgaac ttgcaagcac tgcatcaaca ccttgatgag gaagtgaacc
    15361 atgaccacct ctacctttta cagtaatttt aaagaaatcg gcagaagcca ttcttggtcc
    15421 ttcttctacc gatatagtcc cagattctac atcagtccaa agatgtattc caaacacact
    15481 atctacacct tccatagcac catcttgaat catagctctt gcacctttac caacttcttc
    15541 tcctggctga aaaaataatt ttacagttcc attgatacta tccttaatat catttaacac
    15601 ttttgctgcc cctagaagca ttgatgtgtg accatcatga ccacaagcat gcataagtcc
    15661 ttcattttta gatttgtatt ctacatcagt gcattcaaca acttgtagag catccatatc
    15721 acccctaagt gctacagtct ttcctggatt agctcctttt atagttgcaa ttacacctgt
    15781 acctccagct gatacatatg gtatctctat tttatcaagt tcttctttaa ctctctttga
    15841 tgttctaact tcttccatac ttttctcagg attttcatga aattcccttc taagcttaat
    15901 tacatagtct ttataactct ttgttatttc tttaatatcc attccatacc tccaagtagt
    15961 acacattctt cttatttaaa atacttttta tcacttaaat actatattag atttacaaag
    16021 atacctgcta ttactacaga agctattgta actgtagtaa atcctccaac aagcatcttt
    16081 ggaagcatct tatccattaa aaattctttt tcttctggtg tttcagccaa agcctttact
    16141 gcctcttcag ttaatatata gtttggtgga aaaccataaa gtgatgtaag cgaaactgaa
    16201 aaagccattt ctttactttc acctaaaagc ttacctacta taatagagaa tattcccata
    16261 cctattacac caaatattat gattcctaca agaggaactg ccatttcaac aagcattgct
    16321 ggtgtagctt ttgctaatcc tgcaaatata aaagccatca atgatgtcat taaccatcca
    16381 aatgaaccag atttattaag tggttttcta tcaactattc caagttctga tgctattaca
    16441 ccaaatatta aacatataac atatcttgat acaacttcat ttgtaagatt tgtaaattgt
    16501 actgctaacc atgctacaaa tcctaatcta agtattatca tatatgtcgt atcatatttt
    16561 tctggtaagt ctggaattat cttaaatctg cttttttgtt caggtaaatc cttaactttt
    16621 tcttcatctg tatttacagt tacttttcca cttctaaagt ctgataaaag tctttttcct
    16681 tcttttttaa gcattaatgc tgtaatagga taacccgcaa atccttgtgc aacatacatc
    16741 aaaattgcaa gtactgataa acttgcaaga ccttttgctg tagcagcatc tgccatcatt
    16801 atagatgcaa ctagccctcc agtaagagga ggtgtagcta ttactgctgt atcccatcca
    16861 aatactattg tccctaatgc caaagcacct acacatattc ctactattcc tgccaaggct
    16921 attactattg ttttccattg acctgctaat tccttaatac tcatcatagt tcccatatga
    16981 gttattaaca aaagcatacc taattgagct aatggtgttc ctaaagcggc taagtccaca
    17041 atattttctg ggaaaaaagt ccagaatcct actataaata taattgctga tacaaaaacc
    17101 gacggaacaa aagctttagt ttttgctgaa actatatctc ctatcgtcag tgatattaaa
    17161 actagacaaa aagctgttat tgcctccata caaaatcccc cctcataata aatgtatttt
    17221 tataaaatgt atgtatagtt atccattatt ttactacttt tgaaaaacat ttcaagtcaa
    17281 aattcgattt ttttcataaa attcgataaa ttccataaat taaagttagt tttctaaatt
    17341 attctttttg taaaaacgtt atcctgacca ttggctattt aactctattc ttacaaagag
    17401 aattaaatgt aatttgcaaa aaataaaaca taggatacat tttattcata aaacatctca
    17461 taaatataaa atgaattgtc tttatgaatc ttaatatatc ctatgtttgt aagtttaatg
    17521 cgtatgttag ctatggtgta aaatttgtat actagttatt gtgtaaccta ctatatactc
    17581 catttttgtt tactaactct tcatgagtac ctctttcttc aattccttta tccgtcaata
    17641 ctatgatttc atcagcattt tttatagtag tcaatctatg tgcaacaact aaatttgttc
    17701 tatctttact taattcctct aaagactctt gaatttctct ttctgttaca ttgtctaaag
    17761 cagatgtagc ctcatccaat attattattg gaggattctt taaaaagatt cttgaaattg
    17821 aaattctttg tttttgccca cctgataatt ttacgcctct ttctcctata taagtatcat
    17881 atccatctgg caaagtctct acaaagtcat gtattctagc ttttttagca gcagctatca
    17941 tttcttcatc tgtagcatta gggttcccac aaagaatatt atctcttata gttcctgtaa
    18001 ataaaaatac atcttgttga actataccta tattttttct caatgatttt aaactaacat
    18061 cttttatact ctttccatca atttttatat cacccttatc aaattcataa aatcttggca
    18121 atagattaca taatgtagtc tttcctcctc cagaaggtcc aactaatgct atagtcttac
    18181 cagcttttat agataaactt aaattatcca aaacttgagt cttatcttca tatgtgaaag
    18241 atatattttc tatctcaatg tcacctttta cattttcaag ttcaatagga tttttagctt
    18301 ctttttgatg gtcttgttcc attatctcta tgaatctttt aaaacctgtc ataccatttt
    18361 gatattgctc tgtaaaattt attaattttt tcataggttg tataaacatt tttacatata
    18421 gtatataagc agcaaaatca cccaaagtaa tttttcctat ataagtaaaa taaccaccag
    18481 ctattacaac tacccactct aagatatcca tgtataaatt cataccagag taatattctg
    18541 ccattacctt ataagaatct tgtcttgctc ttctaaataa tttattgctt cttacaaact
    18601 tatttaactc ttctttctca gtacaaaaag acttagttac tttcatacct gctatactat
    18661 tttctaaagt agcatttaca tcacctatag taactctact cttcataaaa gcaatattca
    18721 tcttatcttt tttagctatt gcaaaccaaa ttataaacgg taatatagca aatattataa
    18781 gcgtaagagg aatatttata tctataagta taaagaatga accaaccaac attactatag
    18841 aaatgaataa atcttcagga ccatgatgtg caagttctgt tatatccatt aaatcattta
    18901 ctattctaga catagttact ccagatttgt tattatcaaa atacgtattt ggaagtcttt
    18961 gtaagtgtgt aaatacctca tttctcatat cagcttgcat atctacacca ataacatgtc
    19021 cccaatactg cataaaatag ttaagccctg cttttgctat aaatataagt ataagtgcta
    19081 ctgcaaaaac tactaacatt cttaagtttt tatttggaac aacgtcatcc attatatttc
    19141 tagttatcat aggataaact aaatcacata aggaaaatag aaaagctgct ataaggtcta
    19201 gtgtaaaaat tttcttatat ggcttataat atttgacaaa ttgttttatc ataagtatta
    19261 aatccctcct ttattagtac ttctatatag tacttctaaa aaatacccat aaattagtat
    19321 aacataacaa taactttaaa tcttttataa acttacaaag taataacaat ctttttcata
    19381 aaattgaaga tttatatcat atagttgtat acttgaaaca gcataaaaag agctatatct
    19441 ctatatttaa aattttataa agatacaact ctatatttaa tactaaattt tttttataaa
    19501 cacaaaacta cttcaatgca tttaatatct ttttatctaa tgctgttgta tttatacctg
    19561 ttatatatcc tgttgccttt attgtaggta tatcataagt tgttggtagt atagtagtag
    19621 atacgattaa atcagcacct tcttgttttg ttgaacattc agcaatttta cattgctcaa
    19681 cttctgcgtg tatattattt tctttaacca atctttctac tctgtcacaa actattgttg
    19741 atgttgcgat tcctgctcca catgctacta atattttttt catatattgt cctcctacaa
    19801 tttctttaat attttataac tcaaaataaa attgataaat ttcttatatc tttatttata
    19861 taaataatta tatcatttta tataagaaga acatcttaaa attattcttt taatatgttt
    19921 tttatttcaa cattgcttat acttaaaatt tcatttttta tacaagactc tatgtacttt
    19981 gatatatttt tataatcatt taaattaaac tgttcaatat tcaatttata ttctatatca
    20041 ctgacaatac ctatcaaatc agacttatta gataaaatat cctcatactt accttttcta
    20101 gttacttcca atcttttaaa tctataattt ttatatcctt ctatgataat caagtccata
    20161 tcttcatata attgaatcat tttatataat ggattcttct cttttagttc ttcaatatat
    20221 gctactcgtt tatctgatat tatacagact tttctcgccc cagattttct atgtttataa
    20281 gtatcttttc cttcctcatc catatcaaaa tcatggcagg tatgtttaat aactccaaca
    20341 cgataccctt ttttatttaa ttcttttatt atgccttcaa tcaacgtagt tttacctact
    20401 cctgaatatg agacaactga caatatatta tacataaaat tgtgtccttt cataaaacta
    20461 tctaataaca atctccacta attgattttc ttttattgtc tcattctttt taatcttaat
    20521 catacaatta cttcttagag ttgcagataa tctagctttt gcatctttct tatcatttag
    20581 ctctacatac aatttacctt cacttgtata tagttttcca cttacaaatc tatctttatt
    20641 tgaaagttta ttaaattctc ctttaaatat agcttttata tgatgtattc ccttcttata
    20701 tgttaaatac tcaataacag gaactatcac attatcaaat accactattg ctgcatgtgg
    20761 attaccagaa agaccaaata atagcttttc tcccaatata cctacataac aagctccacc
    20821 aggtttaatg tttatcttcc aaaataattc tgttgcccct atttttttga atacatctgg
    20881 aattaaatct ttatctccaa cagaaacacc acctgttgta attaaaaaat ctacactctg
    20941 taacatattc tttacctttt tttctaactc caatatatca tcagaacaat tgcatattgt
    21001 atctgcctgt attccaagtt ttaataatct tgaatataag gtatatctat tactatcgta
    21061 tattttacca ttctctaaag tctcatttat atcttgtagt tctgtacctg tattcaaaat
    21121 tccaactttt aaatcatcat aaacagttac tttggatatt ccaagacttg ctaatactcc
    21181 aataccatca tatcctatat tctgattttt attaataatc aattgcccct tttttatatc
    21241 ctcaccctta aatatgtaat tatcgtattc ctttaatttt acatttaaaa ttatcatatc
    21301 acctataacc gttacatctt ctttttttat tacacagttt gcaccctgtg gtatctttcc
    21361 accagtcatt atcttcactg cttcattttt atttaatacc ttattaaata cttctcctgc
    21421 atacaaagtc cctataacct ttaaatttct attttcagaa tttgcattat atgcataacc
    21481 atcatagggt gacctatcaa atggaggact atcaattgtt gaatatatat cttctgcaac
    21541 tactttattt actgcatcta taagatttac tatcttgacc ctgtttattg gtaaaacatt
    21601 tttttcaata atttttatgg catcttctaa ttctatcatt ttaaatactc cccaatatat
    21661 tttttattta agatatttct atgtaatata ttatagatta taaaagttgg aaatactttt
    21721 atcttatatt taattataac taatagttat ctataagtgc agttcaaatt ttataaatct
    21781 aataaaaaaa ctgttcccag ctagaaattt ccttctatct tgaaacagca tcatatgctt
    21841 tagaatattt tattctaata aagttccttt tattattttt acaattttat cactttcaat
    21901 agtttttata gattttattt tgttatccac aatacttatt gtaagtacct ctttttttaa
    21961 tattttgtta tcttctttca atctataact cttaactact ttactacctt catgattata
    22021 tgctgttaaa tcataattcg catctcctac aatttcattt attctagact cttcaacacc
    22081 aacatactct cccaggtcaa tacgtctaaa attttctagt gatacagccg atttttcatg
    22141 gtcacttttt tcaatcacaa cactagattc agcatcaatg actgatatac cttgtgatga
    22201 gaaagaattg gtttcaacat tgacaacttt attttcgtta atatcaacat ataaagcact
    22261 tccatctagt tcatcatttc cagtatattt tggataaact aatgtcatat aacttgatgt
    22321 atttatatct tgcattgtat tcgattcttt taaaacttta ggttttatat aataaaatgt
    22381 attataaggt atacccatat tctttataat gtacttataa tctttattca taatttcatt
    22441 aacatcattt tgaacttttg tcatagtttt ttgctgtgtt gctttacttg ctacttgatt
    22501 tttctcttta gtattttctt tatgacatcc aacaagtata atacctaaaa tagttacaca
    22561 aactaatgac ctttttaact tcataaatca atcctccttt cttaaatttt atagtattta
    22621 tctatatctc aatttacagt tatctctttt ttcaataatt atatttatta tattataaaa
    22681 taattatata atatttattt gaatttaata agattaagaa gctaattgta atttttctgt
    22741 aataaaaaac actttaaagt tgaattttga aaattcaatt tacgatattt ttattgtaag
    22801 taccttacaa tattatacca aattattcct aaacaatcaa aaactccatg aatatttaaa
    22861 ggttttctat tttaaaaatc acctttgact attaatttat caaatatgtt ataatagttt
    22921 tagataattt aaggaggatt aaataatgga ttttaaacaa cttgaagtat ttgttgctgt
    22981 tgcaaaacat cagagttttt caaaggccgc aagagaactt tttttaactc aacctactgt
    23041 aagtgcacac atacagaatt tagagagaga gttggaaact gttcttataa atagaagcaa
    23101 caaagttatt acactcacaa aatctggtga aattctttat gaacatgcta tatatatatt
    23161 gaataattgc aaacgtgcaa tttatgatat aaaagaatat tcaggaaaaa tagaaggtat
    23221 tatagatata gcatgtagtt ccatacctga aacttatatt ttacctgatt ttatgaagag
    23281 tttttctatg agttatccag atgtaaaatt ttccataagc cattatgatt ctcaatatgc
    23341 tatatcagaa attttaaatg aacgaataag ctttggatta gttggttcta aaatcaataa
    23401 tcctcaaata gagtacctag acttattaga tgatgaactt gttcttatca ctccttcaga
    23461 ttttaaaatt gataataaaa ataattgtat cgatatagga gaattggctt atctaaattt
    23521 tattatgaga aaagaagggt caggtacaag aaacttaata ctaaacacac ttagtaaaaa
    23581 taactttcca gtaagtaaat taaatgtaat agctcatgta gaatcaaatg aggctattaa
    23641 ggaaatggtt agattaggtc ttggagtctc ctttatatcg tacatttcag ctattgatta
    23701 tctcaatgca ggtaaaataa aatgttataa aataaagggt gttgatttta ctagaaaatt
    23761 tttctttatt tactctaaaa agaaaacttt ttccccatta gaagataaat ttctaaacag
    23821 attatgtgag tatttcgaaa ttataatata ataactttaa taaagtagtg cttaattttg
    23881 ggtttataac taattaataa gctaattata actatcacat aaatgcttat aaattattat
    23941 gaaccatctt tcttttttat ttaatttagt caaaaaatcc ctcgttcaaa tgaacttggg
    24001 atttttttat ttatactatt ccaatagcag ctaatactat acaaataatc ataactgaaa
    24061 ttaatatcgt agttgtcttt accttttttc ctaataacca atacataata ccgaatatca
    24121 tagctggaag catacatggc attatttggt ctaagtaagt ttgaataggc tctgcccatt
    24181 ctccactacc tacattaaca ctaagtttta acattacatt agatgcagtc atacctccaa
    24241 ttaccataag ccctaacatt gaagctgctt ccataacttt tgaaactata cttgaactac
    24301 tcacatcttt aaagaattga gttccatact taaatccttt atctagacat atatagcgta
    24361 taataaaatg aggaacattt attattaaaa ggaataaaat cggtcccatt atgttaccct
    24421 gagaagctaa agctatacca actccagctg ctataagctt taatgttccc cagaaaaatg
    24481 aatctcctat cccagaaact ggacccatta aacttgattt aacagcattt atactattag
    24541 catcaaattc tttattcttt gcattttctt cttccatagc tccagatata cctaccaaaa
    24601 gagtacttat atgaggagta actgacataa attctaaatg tctttttaaa gccttagatt
    24661 tcttttcctt atcatcttta tacaatctac ggattattgg tgccatcatg tatgcatatg
    24721 ctatattttg catacgttcg tagttccaag atgaatctaa tgtcaatgac cttctaaagc
    24781 atttccaaaa gtctttcctc gttattttac tatcgggtga aattgtatct aacttcttag
    24841 aactcgttgt catctactat tacctccttc tctcctacaa agttaacata tattaaagct
    24901 attgctaatc caaacaatgc aactcctgtt actgttatgt ttaggtaagc aaccaatata
    24961 aatcctaata ataaaaatgc tactgttttc ttattaagca tcatcctagc taacatggca
    25021 aaacctattg ctggaatcac gcctgctgca acatccattc catgagttac atatgatggt
    25081 ataaatgaaa gtattttttc tattactgga acacctaatg aaaatcctat acctactatt
    25141 attgctaata ttaatttatt tcctatacca aataataatt gcacacgttc tacgccttca
    25201 atattcccct cttctgcata acggtcagca atatgagctc cccatgtaag tactactgtc
    25261 attaagaagt tatctataag caatactaga gttgctatag gaactgcaag tacaactgct
    25321 gctgatacat cttgacccgt taatattaca aacgctgtgg caataataga accagacaca
    25381 aaatcaggag gattacttgc cccaacagga aatgcaccca taaatactaa ttctaatgtt
    25441 gaacctatta ctatacctgt atgaagatta cctagtgcta aaccaacaag tggtgcaaca
    25501 actataggtc tattatgcat tgtagaacct acaaaactat gcatatatgc aaagaatgca
    25561 attagagtta tcaaaataac ttgtgtaaat tccattttca tccccctaaa attatattaa
    25621 tttcattaca tttgtagcat catcatcagg aactaattgt acaaatacat ttacgccttc
    25681 tgaatctaac tctttaatca tctttatatc atcttttgat acatgcactg ctttagatat
    25741 attttcacga ttatcatctg attttgttcc accaaggttt atagatttaa tagcttttac
    25801 ctctttagca agtctataaa catcttctac agattcacaa agtatcaata agttatattt
    25861 atcagtaaca cctgagttaa gtgcttttat agaatctgct atgcttttca ttactaactt
    25921 aacattagat ggtttagcca ttttaagccc tgccattctt aattcatctt ttaataattc
    25981 atcacttgct atcaatatac agtctgtgct taaaaacttt gtccatgtaa atgctacttg
    26041 tccatgtata agtctgtggt ctactcttac taatttaatc attataattc ctccctaatt
    26101 tttttgattt catccatgaa tgtttttacg tgttctacac taacattccc aaaatctttg
    26161 taattatctt taaagtaact tcctattatt gccatatctc ctaattcaag ctgttttttt
    26221 gcattttcta atgtaactcc agcagctata accaatggaa agtctcctaa atttttacga
    26281 aacagttcta ttttttccat tgatgtttct tgacctgtag cattttctgt aactgctata
    26341 gcatcacatc ttgtcattcc tatttttaaa tcttcttcaa cgtcattttc tgataatact
    26401 ggctgatatt taaaacgtac gcctccaatt aaatacgctg ggcattttga acgttgtaat
    26461 ttaaaaaatt cttctaaagt agcttcgtct ctaggtttaa catgacctac tacagaatca
    26521 acctgtatat aactagcatt atattttgtt gccagttcaa atcccatagt atcaacattt
    26581 aaacaattta ctccatatgg tattgacaaa ttagcttttg atacatattc aagtattctt
    26641 tctaaatcat aataattacc atagtaattt tctaacatta ttccatctac accattttct
    26701 tcaaagattg taatctcttt tttagctctt tcaaagatat cttctggagt atctcctttt
    26761 aagtgtatca ttgctataat tggtttttta cttttaaata ctgataatat tccctttttt
    26821 ttcataaact aaaatacctc ttcttcttct ttaatttgta atgtcacgct ttgtattcct
    26881 cttctagatt cttccactaa ttgttctata tcttcaactg ttaattcatc agaaaacctt
    26941 gtaagaagtt ctaataccat attcaaattc attccagaaa ataacttaac attatgtaga
    27001 ttagttaatc ttaacattgc tgtacataca cttgctccat ataagtcaga taaaattata
    27061 tattcacatt ctatatcttc tgatattgtt ttttctaatt ctaaagcaaa atctgaagca
    27121 ctttgtccag ggtacaaacc atacgaacta acacaatctg ctaaatctcc tactatcatt
    27181 tttactgcat ttaaaagccc ttttgaaaaa tctccatgag atgctaatat tatctttttc
    27241 atacttcatc ctcctactaa atatagatta tatttctgct gatactgcat atttgtactt
    27301 atcacttctt ataaattgct cattgtaata taagacatca ttattttctg tatacaccga
    27361 ttgtcttact ttcaatagtg gcatattact tgcacattta agatagtcag aatattcttt
    27421 gtttgaatgt tttacttcaa atatattttc tactcgttta ggtaatgtgc ttatattatt
    27481 agatataacc tccattaaag atttatcttc taattcttct tttaataaaa acatgtaatc
    27541 tattctaaaa tagtcaactt caaaaataac aggtacttca tctattaatc taagtctttt
    27601 aatacatatt accttctctt ctagttcaat acttaaagct tcagctactt gcttatctgc
    27661 tttttttatt aaaacagaga ttattttggt acttggtttt gcattaatac gatgacaaga
    27721 attagtaaaa ctatttcctg cacatgttgc ttctattctc tctggtattg ttacataagt
    27781 ccctttacca cgttttttac ataatatatc ttcttttaca agttcattaa gtgcatttct
    27841 cacagttata cgacttatat taaattcttt acataagtct ccttcagatg gaagtttatc
    27901 tcctggttta tatatgccag atgctatttg tttttttatg ttgtcatata gttgttgata
    27961 taaaggaaca aaggaattta cttctaaatc catttctttt ttttgtctct tttcatccat
    28021 tttgctcacc tccctatgta acatttttat aacattatat aacaagttct ttattacata
    28081 ttataacaag atgaaatcgt tgtcaatttt tttttaaata atttttaatt tgacagtatt
    28141 ttcttttatt ttctctagtg tgacaaattg ctatttttac ataaaaataa gactgttgta
    28201 caacagatta aattgtttat acaacagcct ttattcactt attacattta tatttttact
    28261 tataaattta tattaaaaaa tgccttatat cccttataca tttcatatat gtctacttta
    28321 gagacaatct catgtggtgc atgcatattt aaaactccaa caccacagtc tataacctct
    28381 gcaccttgat ttgccaatat atgggctata gttccgcctc ctccttggtc tacttttcca
    28441 agttcagcag tttgccatac tacatttcct ttattaaaaa ttctctttac ttctgacata
    28501 aattcagcat tagcatcatt acatccactc tttcctcttg aacctgtata cttagttaaa
    28561 actactccat ttcccatata tgctgaattt ctcttttcat atgcttctcc aaaatttggg
    28621 tcatatccag cagaaacatc agctgataat accttactat ttgctaaagc tcttcttact
    28681 ttaatatcac aataatctcc ttctaaagca atcagttcag ccacagtatt ttcaaaaaac
    28741 tttgagtgca tacctgtatt ccctttagaa ccaatttctt ctttatctac acataatgca
    28801 actgaagtaa attctggttg ttcagtttct agtatagctt ttacagcagc atatgaacat
    28861 accctatcat catgcccata tcccaacacc atagacctat caaaccctaa atctctagcc
    28921 ttaccagctg gaactacttc aatttcagca cttaataagt cttcttcaac tatattgtat
    28981 ttctcattta aaatctttaa tatatttgct gttataggtt ctttttcttc atcttctaaa
    29041 ggaatattcc caattaatat atttaaagct tctccactaa ttccttctga tagtttcttt
    29101 tgcatttggt ctccagccaa atgaatcaat aaatcagtta cacaaaatac tgggtcacta
    29161 tcttcttccc caatagatat gtccactttt gttccatcat ttaatataac tataccatgt
    29221 aaagcaagtg gaagagctgt ccactggtat ttttttattc caccataata atgagtctta
    29281 aaaaaaccta gattactctc ttgatacagt ggatttggtt ttaaatctaa tcttggtgaa
    29341 tctatatggc ttcctataat cttcatacct ttttctatat aatttttacc tattacaaaa
    29401 agtgctactg ccttctcttt atttataaca taaatcttat caccacaact tatgcttcct
    29461 ttttccatag cttcatctat tgaaatatag ttattttctt tagccaattt tataatttct
    29521 tttactgaaa gtctttcagt ctttgactta cttaaaaact ccatataatt gcgagaatat
    29581 tgcataattt gttcaatagt attttctttt ctttcaaaat cccaagcatt cttaaattcg
    29641 tactttaagt tcatattttc ctcctactaa gttctaattt ttattgtttt tcatgtattg
    29701 ctcatattct tctggtgaaa ctactttgac actatctagt cttgatctaa agtgagctct
    29761 aaagttttct aaatattctt tccttaaaat ttctctttct ttgatttcat cagcagaaag
    29821 gattccctct ttatttttct ttgctaattc attaatccta tctaatttct tcttgtcaaa
    29881 cattttttcc tcctaaaaaa cctctattta tttaataaaa taatttattg ctttttatat
    29941 ctaacttgtt tttatattca caaaaaaact catctatatt atcacttatt acagatattc
    30001 ccttttctag ttcttccatg cttgggcgag ctatatttat cctaaagaat ctatcatcca
    30061 ctagattgtc aaaaaaataa gttcctggaa gcattgacac tcctttgttt aacataaaat
    30121 ttgtaaatgc ttgagatgta tatcctcttg gtaattctaa aaaaaagttt attcctccac
    30181 aagattttct aacttttaat ttaccactca atttgtcatt aatcaagttt tttgacaatt
    30241 tatacttatt agtatatatc ttctctatat gactcaaata attcttccaa tcaaaattat
    30301 tcatatagta atacattgat ttttgaatta aacctggtgt tgatatatct gatgaatatt
    30361 tagcccataa aacttttttt aacaactcat ttggcatttc tacaattcct atcctaagac
    30421 ctggcataag tatttttgaa aagcttttta tatatactac tctattttta tcatcataac
    30481 ttctaattgt tctattgtcc tttgattcaa aaacaaaatc acttataaaa tcatcttcta
    30541 ttatataaaa atcatgttct tctgccaatt ctatcaattt ctttttttta tatgttgagt
    30601 aagatattcc agttggattt tgaaaattag gcataatgta tataagtctt ggttttattt
    30661 tttccaactt taattttaaa atacctatat caattccatc atctaacata ggtatagata
    30721 ttatttttgc acctctactt ttaaacactt caattgctcc attgtaagaa ggttcttcca
    30781 taaaaacaac atctgaataa cttataagtc ctttacaaac tatatctata ccttgttgag
    30841 cacctgatat tatttgaata ttttccttag tagtttttat atttccatca cttagatact
    30901 ctaccatttt ttctcttaat tcttcagagc caagaccttc atcatattca aatattgaac
    30961 taccatcttt agataatgcc atatttacag catttttaaa tgaatcgata ggaaacatat
    31021 cattagatgg attcccattg tcaaaatgaa ttaaatcttc tctttgcaca ataatcttat
    31081 ttttcctgtt atctataccg cttttataag aatttgaact tgatacaaaa gtaccactcc
    31141 ctataatttt atatacatat ccttcatttt ctaaaagctc gtatacctta actatagtag
    31201 tgttatttac ttgtaaaaaa tttgatagtt ttcttattgg aggcaatttt tcatgttcta
    31261 ctaactcttt atttattatc aattctttta tatgattaaa tatctgtata tatttagttg
    31321 attttttctc atttaaatct attctgtatt tttccaaatt tatcaccgct cattgtattt
    31381 atattttatt gatgctttta tatatatttg atattattta gtatgtatat ataataccaa
    31441 aatttttata aaaaggggaa atatttatgt tttacgacta tcacatgcac tcaagtttct
    31501 ctacagacgg aaaatctaca atggaagaaa tggtaaaaaa atctattgaa ctcggtttgg
    31561 aggaaatttg tttcacagac catgttgact atgatgtata tgctgatgat tctttttcaa
    31621 ttgtctacga agattatttt aagagtttag agactcttca aagcaaatac aaagataaaa
    31681 tatcaataaa aaaaggaatt gaatttggag ttcaaactca attaatggat acatacaaaa
    31741 aggaagcaca tcaatatcca ttggacttta taatatgttc tatccatgct atagatacaa
    31801 tggacttata ccttggtaat tattttaaag ataaaactca atatgaagtt tatgaaaatt
    31861 actatcttta tttatataat attgttaaga attataaaga ttactctgtc ttaggtcatt
    31921 tagatttgat taaaaggtat gcaccttatg acactatttt agatgataga ctattttctg
    31981 atataattga agaaactctt aaacaagcta tttatgatgg caaaggtatc gagataaata
    32041 cctcttgtta tagatataat cttccagacc taaccccatc taagtatata cttcaaatgt
    32101 acaaagactt gggaggggaa atcataacca caggttcaga ttcacatcat ataagtcaag
    32161 ttgcatgtga gtttgactac atatattcat tactaaaaaa tatgggattt aaatatgtat
    32221 ctaaatttaa taaattgaaa ccagaattta taaaattata aatttttaca cctccttgtg
    32281 aatatattta aaagataagg aggtgatttt tttgaaagta ccttatttag tactatcaac
    32341 agttatttta tttatatcca attttattgt aagaatattt ggttttttat ataaaatatt
    32401 cttatcaaga gcactaggtg aaacaggttt aggtatctat catatgattt ttaacttttt
    32461 gatgatttgc ttagctgtta caacaacagg tatacctact gcacttagtt gtctagttgc
    32521 aaagagaaaa gctttaaacg atagacacaa tactaatgct ctatttatat caactttata
    32581 tatatcattt ttcgtagcac taattatatc tatagttgca tctttcaata gttctttttt
    32641 atcacttaaa tttttaaaag atgctaaatt aaatctattt atactagctg tttgtcctgc
    32701 tatagttata ataactcttt caaacgtact aagaggatat tactatggca taaagaatgt
    32761 aaaaatccct gctattggac aaatcattga acaaattggt aaaatattat ttgttttttt
    32821 acttgttatg tacataaata ataaatctat gaattgttat atagctctat taggtatatc
    32881 tattggtgag ttgagcaata ttatatttat gcttatttgc ttatggcgag attcatcatt
    32941 tgacaatagg tatattataa gcataaaaga tttttataat tcttctatgg aaacattaaa
    33001 aatgtctatt cccataactt gtaatagaat gtcaaacata cttttgcaat ctataagttc
    33061 tatgatgata ccatcaagat tagcattatc aggaatgact tatcaacaat ctttaagtat
    33121 gtatggtatt gtcagtggga tggtaatgcc ctttatattt ttacctttta ctgttggttc
    33181 agcgcttatt gtaaatttaa taccaactat ttcacaggaa atggctctaa gaaagcgtaa
    33241 gagtgtaatt aaaaaaatca aatactccat acttcttact ttatttgtag gcatactatc
    33301 atcaatattt ttttatttct ttggaaaaga tttatgtata ttagtattta aaaatacact
    33361 tgcaggagaa tatctaaaag ctatgttttt agttccacta tttatgtccc taaatcaaac
    33421 tctatctggc attttacatt caataagaaa agaattagct tcaagtataa atactattac
    33481 tggcatgtta attcaactaa tagctttata tgtatttctt ccaattccag gacttaatat
    33541 ttatgcatac atatacacaa tgacgatagt atcaatcttt acttgtctat tacatactat
    33601 agtgcttttt aaatcactaa aatctatacg ctagttataa aaacgtgaag gctaccaatg
    33661 taaattatac actttggtag ccttccttag atgattttta tttataattt aaactgtcta
    33721 tatatcttac ctatctatct gtggcaactt ggctttccac ttccatgatt aggtccacat
    33781 tgcgaaaaat cacatacttt agagaaagta tcaacaagtc ttggttggat tggatttggt
    33841 gatgctaaaa ctactatttg ttcacttatt aatgtgctaa ctgtttcttg taagcaaagt
    33901 actaaatcta tatcatttgg tataattaat tcatagaaag gttcacaaca ttcgttgcat
    33961 tctaatttta atatagattt tcctatacaa tctacagtta agttagtttg atattcttgt
    34021 cttaaagtaa cagctcttcc gtcagctggc aagcatattg gtgctgaaag tgtgttaaaa
    34081 gtaaattcta cataatcaac tcttctctta catccacctc ctgctctaac tgctgggaaa
    34141 gctaatgcaa cgaactctgt acatccacat ctacctctta gctctagtac taaattacga
    34201 actgctacag ttaatcctct ttgtttataa gctactgatt tgccttttcc ttgtctacag
    34261 cactcagcgt ttctttctcc acaaactgca aattcaaaag ttgtttcaca gtttcttcct
    34321 actatatctc ctaatgggtc taaatcgaaa tcttctaaag ttgtcattcc tgtgtctata
    34381 actattccat cattacttaa gcatattttt tctatagtta cacttgcttg acctgcactt
    34441 ggaattgaac caaatacttc tactacttct gtttcaaaag ttaatggttc tccatttgga
    34501 cctgttgcat ctgtaaatgt ttggaattgc atagtatcaa atattctatt agcctctatt
    34561 gcaaatggca ctgcatttga tacagagaag tttcttggag atatcggatt acaacattct
    34621 ggtccacatt tgttttcaaa acattcatct gaatccattt cacatggttc acaatcacat
    34681 ttacacttgt catggcattc acatttgtca tcacatggat taggtttgca tggattgcat
    34741 gggttaggtt tacatggatt acatgggtta catggattac actcatcatg gcactcacac
    34801 ttattatcag tattacatcc attatgatgg tgtgattttt taaaccctct catgtcttct
    34861 gaatttattt catctgtata cacttcttct tcattcattg tagacattgg ctgattcatc
    34921 attcttcttt tatttttttt ataatcttgc atttttcatt gccctccctg taagcgattc
    34981 ttattaagtc tttggctttt tgaagcctaa ttaatactat gcttttataa tcttaattgt
    35041 tacttgcttt ttgaatttat caataattag gcgtatttag tagaaaaatg catattaata
    35101 aaaaaagtac tactatttga aatagtagca ctttttttgt cccgaaacac cggtggctaa
    35161 gtaaattcac accctaggca ctcccaggtg ggtgacttgc cagtaacctt taacgtccaa
    35221 actaactagg catgttataa tttaccagac acggtctccc gtatttcatt tgtaggttga
    35281 atttaaaaaa gccttatcgt atgtttcagg atttcatatt attattatat aataaatttt
    35341 gaatttttta aagtggtttt atatagtaat tgagataatc ttattctatc attacttttt
    35401 cttccttttt tctttatgta tttagttaat tatcgttttt tcttagctct agttttagta
    35461 tttttttcat tataattgta ttcggattct tgctcattaa tgttgtatag ttcctcatcg
    35521 tatactatat cttcatcatt ttctatattt tcatagcctg tgttcttatc ttctactaat
    35581 tcatcttcat tatcctccat ttcaccttca tctgttaact catcatcatc caattctttg
    35641 tcttcacaat ctaattcttc gtcttcataa tttaactcat cattattgtc ttcatcatca
    35701 ttattactat catcacctct taaaagactt cctaacaatt tgagattatt catactacca
    35761 agattgttta tatttgccaa agagctcaaa ctaaatccac cttcaccaaa gtcatctggt
    35821 aaaaataatt ctgcaaataa atctatcttc tttttcattc ctatagcttt ttcaactgaa
    35881 ttttttcttt tttctgggaa tacatctata atttccttta tcatcatatt ctttttatca
    35941 tcactatcca tttttctgaa aaaatccgtt tcatcttctg tatctaccat atctatatta
    36001 tttaattttt ttattcctct aactaaatct aaaacacttt ctatttttat tagtattttt
    36061 ctttcatctc ttgacatgta ttttttagtt cttgttatta tttccattcc catttctaaa
    36121 tcttcttcac ttaaatccat gagccttaaa cctcttttta tatcatttgc atcaaacctt
    36181 aaattgccat gtgtagactg tgaaagttta tcatttgcat tttttttgtt ttttctaact
    36241 ctattatttc cttttgattt atttttatca tcaaatatag agatattacc attactcaat
    36301 gcaatcactc caatcaaaaa tagtaagtta ttcaagacaa ttccccctta tattatataa
    36361 ttgccctata ttaaagtatg tttttcatat tttaatgttc catttttaaa gctcagtgca
    36421 tatatattat ctataagaat ttttatttat attatatatc actcacaatt caccacttca
    36481 atattatttc tatatataaa tagtatgaat ataattcaat atatatttat agaaaggggt
    36541 ttttttatga aaaaattaga tacagagtca ttagaaaaaa tggctaaaca aaaaaatatt
    36601 gacaaggata aaatagaaaa gatggctgat agttacaaag gaaaatcaga aaatgaactt
    36661 atggaggaac ttataaaaat aggtaagaac ttagatggaa gagatgaagt agtatcaaaa
    36721 ttcaaagcat ttttagatga aaatcaacgc aaaaaactag ataatataat ggaaaaaatc
    36781 tcagatgcag aaaatcaaag agatactaaa ccagctaaaa ctaagaaggc taaacctact
    36841 aaaggtaaat caaattcacc tgcaccaaaa aatacaccag gtggtcaaca aaaaaaatct
    36901 aaaggtttat ttaagaaaac taaaaagtct aaccctcatg agtagataaa aataaactat
    36961 ttaagtaatt agataattat ttttagttaa tctattatta aatttgatat tattgacata
    37021 aaaaatcagg tagtgcctta ggcactacct gattgatttt aatagtttta tttttttctt
    37081 tctgtaacac tttctctatc aacaattcta taaggtaatt ctattttctt attttcaatt
    37141 aattccttat ttattaattt tataaccatt cttattgcta ctgctcccat atcgtataga
    37201 ggttgatata ctgtagtaag tttaggtcta tacattctag ctatcttaac atcattaaat
    37261 cctgcaacag atatgtcttc tggaacctta tatccagcat cacatatagc atttatagca
    37321 cctatggcag cctcatcacc tgttacaaaa gccgcatgag gtattattcc atcatctaat
    37381 agttctttca tactattgta tccactctca taatcagttc caccatactt aatcaaactc
    37441 ttgtctaatt ctatattgtt atttgaaagt gctttctcat aaccagcaag tctttccatt
    37501 tctaaaacag tatcatcttt actcgtcatt ataaaagcta tcttttcatg acctttcttt
    37561 ataagatgct ctgtcatatc aaaagtagct tcttcgttgc tagtacttac tgtatatata
    37621 tcataatttc tagcagtttt acttatatat gtcgctggta ttccacaatt ttgtatatag
    37681 ttgatatgtt cttgttcaac tatccatgaa atcataacta taccttccac ttgttttgct
    37741 ctcaatagat ttatactctt aagttcttgt tccttatcag agtatgtatt cgctaaaaga
    37801 atatcatagt catacatttt agcaacttct tctatcccat ttaacacctc attaacaaaa
    37861 gaatctgaaa cttctggaac tattacccct attaattgac tcttctttgt tactaagctt
    37921 ctagcaagtg gatttggtat atatccagtc tcttttataa cctctaaaac tttttgtttg
    37981 acttcatcag ttacaggttt tgaatcattt ataactctag atacagtaga tattgacact
    38041 cctgcttgtt tagcaacatc ttttatcgtt atattgcctt tcatcttcat cctccttatt
    38101 ttactaaatt tatctaaaac tgaaccaaat ttttgaaggt tcctatttta actacattta
    38161 tataagtttt tattttacca taaaagcaag tacatttaaa tacattatat tttatattat
    38221 accctatatt caacaaattt aataatattc attcttaatt taaatcatat taaaaaataa
    38281 aataaaaaaa tactggtata aaataccagc atttttttat tctaatgatt taagtattag
    38341 cagccgcaac ctacttcacc atcaaagaat ccaccacatg aacaagttat tagcaataat
    38401 attaagaaag gtatccatga cattatgtca atgtctctcc agcaatcttc aaaagctaag
    38461 aataggaaga atagtactat tatccaccat cctcctccac caaagcatcc ttcttctcca
    38521 aaaaatctat ctaacataac aaagccctcc tttttttaat aactgtgttc ttattctaag
    38581 catccaccac agcagcaaag tattaataaa actattagga atggtatcca tgccattatg
    38641 tcaaattctg cccagctatc tccaaaagct aagaatagga agaatagtac tattatccac
    38701 catcctcctc caccaaaaca tccttcttct ccaaagaatc tatctaacat tgtacgttac
    38761 ctcctgttga tattttgttg agcttttcgc tcctaacata tactatgcat ttggtttcca
    38821 ttttgctaat gtatttttac tattttttta aattttttaa tttgaactaa aaaaggtaaa
    38881 accataactt gtataagtta ctggttttac ctatttaata atcaatattt tttagcaaca
    38941 acaaccatca tcgaagttaa ataataacaa tactattaat ataacccata tccatatatt
    39001 gtcatcgcat aataagtctt ctaaaatgtc tgtgcataat agtaagaata ctataactat
    39061 tattatcaac attttattac caaaaaatcc ttcgcatcct ccaaagaaac gttccatatt
    39121 gtcttctaat tttgtcataa ctaatcctcc ttgaatcatt aagagactaa gttttgtctc
    39181 tttaattaaa tatatgtagt aatagacaga taagttactt ctaaaaagtt tttattgatt
    39241 agtttattta tttctttttc accaagttta ttgtatctat caacttcatc cagataatct
    39301 tttttctcct ctgatatagt gtagattaaa ctttctattg attttatttt gtactcctta
    39361 tatctttcta tcaagaagga tgtttttgaa ttttctattc ctttctttac atgcattaac
    39421 cttgatatgt agtcttcttt ttcattagat acataactct ccttttcagc tacatcttca
    39481 aggattaaat tggtctccat tacataatcc tctatacgtt gcatatctaa agttattgaa
    39541 ttataataaa aatttactgc tataagtaaa aacattccta ctgaagttag taatagtcct
    39601 acatatttca tagcatttcc tcctaagtta tatttgttat caataaagtt tattcatcaa
    39661 ataatacttt attactacat ttataaaatt ttattatttt ttatagattc catattgaaa
    39721 aattcttgat attatattaa actaacatta aaattaatta aaactgcttt aggaggctta
    39781 aaatgtttga gaaaataaaa gaaataatag cagaacaatt aggggtagac aatttagatg
    39841 agataactat ggaagcatct ttaatggacg acttagaagc tgattcatta gatgctgttg
    39901 aaattataat ggcccttgaa gatgaatttg gaatagaaat accagacgag gaagctgaga
    39961 actttaaatg tattggtgat atatgtaaat atatcgaaga aaacaaatag tatcgttttg
    40021 tctacaaata taaatagtct gtgtaaactt ctacataata aattatatta gagttcgcac
    40081 agactagatt acatttattt acaatatata aaatacacgc tcttatattt aatttatata
    40141 ctaagtatat aaattacttt tcttcataaa atgtaacatt ctttattatt atatctatag
    40201 ttccatcttg attttgcttt acagtatact tcattgggtc ttcaaaatca gttagattcc
    40261 cttttatgtc aaaaccatta tccgttttta tacttctttt tttaagcttt ttctcaaccc
    40321 attttttatc tatactaaaa ctttcatcaa gacctttttc ttccatatgc tctttaaagc
    40381 tatcctttaa ctcatcatct ttaattgaat tatcaacaaa gtcatttata tcaacttcat
    40441 gtttttcctt cagtgtataa tttaatatac ttcttacatc ttcagcttgc tttatatcat
    40501 tactaagagc attagttatc caattttcag ctgtattttt aaataccttg gtcttgtact
    40561 tatcatcatc tattttttta gcatttaaaa attctgttac aaacttagat tctgaccctt
    40621 ctttttcagc atccttatct aaaagcctta gatgaaactc atcatttata ccacttaaac
    40681 taatcaaagc tccttgcttt tgtcttaaag tctctgggat accaatttca tttgaaacca
    40741 tttgaatatt aaatttatca tccacaaatt caattgaatg agtgtacaac tttttataat
    40801 ctaatttcag tattgctaca ctcttttcat ctttaatagt gtataagcaa attgccaaat
    40861 cacaagattc tagtgcagca ttaattttca taacgtcaaa caagtaagat gcaatttctt
    40921 tagaattttt caaaaaagta ctttcatcat atattatttg ttcacaacag tttttaatta
    40981 aattgtcatt ataatcttta aaaacacctt ttcttaaatc ctcatctttt gcaattcttt
    41041 ttatagcttt ctggaaaaaa ccatctactt cttgattaac tttgccttca aagtcattta
    41101 atattggaac atcactattt ttgtccaaaa catgtattat aaatttatgt attatcatat
    41161 atatcacccc attatcttaa tttcgatatt aactctttca tatcctctgg tatatctgct
    41221 ttaaatttca atttttcttt tgttcttggt tgttcaaact ctagcttgta tgcatgaagt
    41281 gcttgcctat ttattagctc ttcatctaca taaccataca actcatcacc tattattcca
    41341 tgacctatat gagccatatg tactcttatt tgatgtgttc taccagtttc taagcttact
    41401 tctaatacag ttgcattttc tagtctttct attactttat agtgagtaac agatgactgt
    41461 cctctttcat ctattatcct ctttattgaa tcttctgtag gtctataaat tggctcatct
    41521 atagttccct cattttcttt tataattcca tcaactactg ttatatactt tttttcaact
    41581 ttattttcac tcattgctgt agataaagta tgatgtgcat aagcattttt tgctactata
    41641 actaaccctg aagtattcat atctaaccta tttacaaatc ttatttttac gttttctttt
    41701 tgattatcta tatagtaact aataccattt gctattgttt tatcatagtg gctttttgtt
    41761 gggtgaacta ccataaatgg aggtttattc accataatta tatcaaaatc atcataaatt
    41821 atctgtaaat ttaaatcttg aggttcaaaa tttgcttttt cctcgtctat ttttacttct
    41881 attaaatcac cactatacac ctttaaactt ggttttttat atactccatt tactaaaaca
    41941 ctcttttcct tcttcatttt tgataatgac ctaactgaga aatttagctt gtccaataaa
    42001 acttctttta aagtcatttc ttcttcattt gtatacgata taagattata tctttgattc
    42061 tcttttttaa acaaactttt acctactttc ttttatatct tcctgctttt tatatcttca
    42121 aaatcatgat attatatatt ttacactatt tttgtttaat agataataat tatttttaac
    42181 tataaaaacc tgtaagaata acaaatagtt attcatacag gttaaattca taattaaata
    42241 tttaatttgt aaatattata atctaaaact atctcttaac tattaaagta gttcccattc
    42301 caccacctat acaaagtgta gcaagaccag ttttagcatc tcttctcttc atttcatata
    42361 aaagtgtagt aagtattctt gctcctgagc atcctattgg atgtcctata gctattgctc
    42421 caccattaac attaacttta ttcatatcta tatttaagtc tcttattaca gctacagatt
    42481 gggcagcaaa tgcctcatta gcttcaacta aatctatatc ttcaatagtc atattagcag
    42541 cttctaaagc ttttttagtt gctggaactg gtccatatcc cattatttta gggtcaacac
    42601 cagctgttcc ataagaaact atagttgcaa gaggctctat tcctagttct tcagcttttt
    42661 ctttagccat tactactagc atagcagcac catcatttat tcctgatgca ttaccagcag
    42721 taactgttcc atctttttta aatgcaggtc ttaacttagc aagtttttcc attgtagtgc
    42781 caggcttaat atattcatct ttatctacta cagtgtcacc ttttcttcct tttataacaa
    42841 caggaactat ttcttcatca aattttcctt cagcttgagc tttttcagct ttattttgac
    42901 ttgcaagagc taattcatct tgttcttctc tagttatatt ccattgctct gctatgtttt
    42961 cagcagtaat acccatgtga tagttattaa atatgtctga taatccatct tttatcattg
    43021 aatcaacaaa agcagcatca cccattcttg caccatatct cgcacttggt actaaataag
    43081 gagacatact catattttca gctccaccaa ctaacattat atcagcatca cctaatgcta
    43141 taagttgaga tgccattgaa acagatctta atccagaacc acaaactata tttatagtca
    43201 tagctggttt ttctactggt attcctgctc ctaatgctat ttgtcttgct atattttgtc
    43261 caagacctgc tgtaagtact ccccctaaaa gagattcatc tatcatatct ggagttatgt
    43321 tagctctttt tatagcttct ttagctgctg ttacccctaa ctctaccgct gaaactgatt
    43381 taaatgctcc tccaaaactt cctactgctg ttctagctgc actggcaatt actacttctc
    43441 tcatattcat tacccccatt aaattagatt tttcatatat ccgaaattgt tttttaaaca
    43501 attttattta aacttttaat tcccaaattc ttatttatta taatcataga atcctatctt
    43561 agtttttctt cctaattgat tagctctaac cattttagct aaaagtggat gaggtctata
    43621 tttagtatct ccaaattcag tatataaaac gttcattata gctaaaacaa catctaatcc
    43681 gattaaatca cctaatgcta gtggtcccat tggatggttt gctcctaatt tcatagcttc
    43741 atctatttct tctttacttg caacaccatc tgcatatata ccaacagctt catttatcat
    43801 aggtataagt attctattta ctacaaatcc aggagattca gatacatcta ctggtacttt
    43861 attgatactc ttagataatt caaatactgt atcaaaagta acttttgatg ttaactgacc
    43921 acttataact tcaactaatt tcatcatagg aactggatta aagaaatgca ttcctataac
    43981 tttatctggt ctcttagtag aagaagctat ttctgttata gataatgatg aagtatttgt
    44041 tgccaagata gtatcttctt tacataattc atctagtaac ttgaaaacat ctttctttat
    44101 attcatgtct tctacagatg cttctattat taaatccata tcttttaaat cttcataatt
    44161 agtagttgaa ctaacatgac ttaatatttc tgcttttgta gcttcatcca tttttccctt
    44221 agtaactaac ttagttaaat ttttatctaa taaagctaaa catttatcta tagcaccttg
    44281 agttctactc tttaaacata catcatgtcc acaacttgca aaagtttgta caataccact
    44341 tcccatagtt ccactaccta ttacagctaa tttcatagtt ataacctcct gttattttag
    44401 tatttatatt ctgttaacta gattaaaata ctatttaatc tagttaacag caaatatatt
    44461 tttaattaga tttttaccct tttataaagt tagcttctct cttttcaacg aaagctgaca
    44521 ttccttcttt ttggtcttta gttgaaaaac aaagaccaaa taaattagat tctatatcta
    44581 ttccagtatt tatatcagtt tgagcaccaa gttgtattgc ttctttagag tatctaactg
    44641 caagctgagc atttttagct attatcttag ctaatttctc aacttcttct attaaaatgt
    44701 ctggctcaac gactctattt actagcccta ttttttcagc ttcatcagct tttataactt
    44761 gacctgtaaa gattaattct tttgcttttg ccattccaac caatcttgta agtctttgag
    44821 ttcctccata tcctggagtt attccaagag ttacttctgg ctgaccaaat ttagctttag
    44881 cagatgcaat tcttatatca catgccattg caagttcaca tcctccacct aaagcaaatc
    44941 cgtttacagc agctatcact acttttttac tattttctat ttctccaaaa gcttttgctc
    45001 ctaagatact aaaatcttta gcagctacag catctaaatc tttcatgtat gcaatatctg
    45061 ctccagctac aaatgccttt ccttcccctg tcaatattac aacatcaata gtttcatcat
    45121 tattaatatc tacaaatact tcataaagtt cttctaaagt ctttgaattt attgcattaa
    45181 gggctttagg tctattcatt ttcactgtac atatatttcc atctacttca acagctacat
    45241 tctcataaac tttaacatca cttgtactca taataccctc cccaagaaat tagttagcta
    45301 aaacttcacc tttttctttt gcaacactta actgactgat aagttctgga agcactttat
    45361 gaacatctcc aactatacca acatcagcat atttaaatat tggagcttct ggatttttat
    45421 ttatagcaac tataaactca gcatcttcca taccagctat atgttgtatt gctccagata
    45481 taccacatgc tatataaagg tctggtctta cagttttacc agtttgacca acttgtcttg
    45541 ctttatctaa ccaacctgca tctatagtgg cacgagaacc agaaacttct ccacctataa
    45601 tttcagctaa ttcataaagt atgtctaagt tttcttttcc acccattcca cgtccagcag
    45661 aaactaatat cttagcatct tctattttaa cttgtttttt agcttctttt attacttgta
    45721 caacttgaac taatttatca gcatcattaa attctacctt gaaacggtta attacagctt
    45781 ctttagtttc atcaggttca tttttcttca taacccctgg tctaactgta gacatttgag
    45841 gtctgaaatc tttacaaact attgttgcca ttatatttcc accaaaggca ggtcttgtca
    45901 ttaataataa ttttgtatct tcagctactg caagacctgt acagtcagca gtaagacctg
    45961 tatgtattct agcagaaact ctaggcgcta aatctctacc tattgaagtt gcaccaaata
    46021 atacaactat agggtcagct gcttttattg cttcataagc tgcttttgta tatggttcag
    46081 ttgtatacac tgctaaagct tcatcatcta ctactattac ctcatctgca ccatagtgtg
    46141 ctaatgtatc tattaaacct tctaccttac tacctaaaag taatgcagaa acttttgtat
    46201 cataatcttt tgctatttct gtagcctttc ctagtaattc taaagaaaca gtttgaatta
    46261 cattttctct ttgttctatt actactaaaa cgttacccat tctttatccc cccttaaatt
    46321 atatgatata cttctctttt aatttatcta tgataattcc agctgatgtt ttcgcatctt
    46381 cattgtatat tgtaccagct ggtttaactg attttgtaaa tgatttaaat acactagttg
    46441 gagaaccttt aagacctaaa ttagaagggt caacttctat atcttttaca gtccatgttt
    46501 ctactacatc attttcgaaa gcatcatata ttcttccaac tttcatgtat cttggtgtgt
    46561 tcatatcttt aagagttgtt ataaggcatg gcattttaac ttttaagtca tggcaacaat
    46621 cttcaaattg tctttttact aatacatatt caccttcagt ttttatttct tcagcatatg
    46681 ttattgatgg aagatttaaa tgttcagcta tttgaggtcc aacttgtgca gtatctccat
    46741 ctatcgcctg tcttccagct attataatat caaaatctat attttttaat gctcctgcta
    46801 atgctgatga agttgcccaa gtatcagcac ctgcaaatgc tctatctgtt aataatatac
    46861 ctctatctgc acccattgct aaagcttctt ttaaagccat atctgcttga ggaggtccca
    46921 ttgttataac agttacatga gcacccattt cttcttttaa ttttatagct tcttctaaac
    46981 ctgctttatc atcagggttt attatacttg gtactccatc tctaattaaa gtacctgtat
    47041 taggatctag tttaacttct gttgtatctg gaacttgttt tatacaaacg actatattca
    47101 taaatccttc ctccatatct attttaatat cgacctattg tgtatatttt aatatttatc
    47161 tattttaata attttcctga aataaccatt ctttgaactt cactagttcc ttcatatatt
    47221 tcagttatct tagcatctct catcattctt tctactggat agtcacgagt gtatccatat
    47281 cctccatgaa gttgtacagc tttcgtagta acttccatag ctgtttcagc tgcaaataat
    47341 tttgccattg ctgcttctac tccataaggt tttcctaagt ctttatttat agctgcttga
    47401 tatacaaggt gtctagccgc ttgtacttta acttccatat cagctaattg gaattgggta
    47461 ttttggaatt ttgataatgg tctaccaaat tgtactcttt cttttacata tttaacagtt
    47521 tcatcaagag caccttgtgc taaacctaaa gcttgtgctg ctataccaat tctaccacca
    47581 tcaagagtag acattgctat cttaaatcct tgaccttctt ttccaagtaa attttcttta
    47641 ggtattctgc aatcctcaaa tattaattca ctagtagctg aacctcttat acccattttc
    47701 ttttctttaa ctccaaagct aaacccagga gttccttttt caactataaa tgctgatatt
    47761 cctttgttcc ccttagattt atcagtcatt gccattacta catatatgtc accagctatt
    47821 gcatttgtta taaatatttt tgagccatta agtatgtatt catccccgtc taaaacagca
    47881 gttgtttgtt ggccagacgc atctgtacca gcattaggct cagtaagacc aaatgctcct
    47941 aatttatctc cacttgctag tggttttaag aatttttgtt tttgttcttc attaccatat
    48001 tgatatatag gccatgagcc aagagatgta tgagctgata atataactcc tgtagtacca
    48061 caaactctag acaattcttc aactgccatt atatatccta cagtgtctcc accttctcca
    48121 ccatattctt ttggatatgg tatacccatc attcctgctt ttgccatttt ttccactgtt
    48181 tcataaggaa atctttcttc ttcatcaagt tctgttgcta aaggtttaac ttcattttca
    48241 gcgaagctta catatagctc tttaagcatc tgatattttt tagaatttaa atccataata
    48301 gcctcctcag tattttataa atattttatt ttcatttatt aattaaaatc tcggcaacat
    48361 ctataataag gtatttgaat atacgtcgag aaatttataa ccttaatcaa actacgctct
    48421 atatttatat gtgattatac taaccaatta tgacattaat ttaatcataa attgttaatt
    48481 taaaatcaac tatacttaaa aaaaatctcg ctttttggaa ttttgtgaaa tttcaagtta
    48541 gaataagatt tctttcacac tggtcacaga taaaataatt tgtttacgat atttatatta
    48601 gggtatatta aacattaaat attgtctgta catctgtttt ttagccatgt acagaagatt
    48661 tttatcgtat aaaaattatt ttaatagagc taaataatcg tatttttatc tgactatttt
    48721 ttatttttaa atatcttaca tctataatag taacactcaa aggataattt ttcaataatt
    48781 tctaattatt tttttaacaa tatttattac attttttcca cttttttgtc atttttttat
    48841 tgtttcgaaa aaaaatcaga attttttaac tttattttgg attattagtt taaattagat
    48901 tttcctctta gatttataaa tatttacaca aaaaactacc tctgtataga ttgtatacag
    48961 aggtagtttt ttactaattc ctactatagt ttactatatt tttttaccaa ttcttacaaa
    49021 catctaccca tttttcactc ttagaaaact tctccatttc ttcacatgta agttctattg
    49081 cagagttact actgccacat gcaggaaata ctgtatcaaa tctcttcatt gaatcatcta
    49141 aatagacttt tatagaattt tttaaaccaa atggacagac tccaccaata gcatgtccag
    49201 taaactccaa gacttcttct ggtgtcaaca ttttagcctt acaattaaat tcagccttat
    49261 acttcttatt atcaatctta gcatcaccag ctgtcacaat taaaattgca tcatctccta
    49321 ttttaaatga gagtgtctta gctattcttg ctggaataac cccagctgct tcagctgcaa
    49381 gttccacagt agcactagat tgttcaaatt ctaatatact gtcttccttg ccaaattgtt
    49441 taaaatactc tcttacatta cttattgaca ttaaataccc ctcctagtct gaatttttat
    49501 aaatttcata ttattcttaa ttatatctat aattgattta tttttcaaat tattccataa
    49561 tattagcatt ccatgtatca ttattttttc caactactag ataaagtcct tttgccattg
    49621 gaatattgac aatatattct cctttatatt ctacactcat accagattca ttaaagtacc
    49681 tataatcaaa atttagtcct tttggaatta caaaatcaac ataaccttga ttatttggtt
    49741 ttattccata tttcagagta ctttcagtta tagaattttt ttcattaaat aaatctacat
    49801 ttacatatgc agaacactta ttcattccct ttttattttc cacacaattt tcaaacttca
    49861 ttttagcact gtttaattta taactatatt tactattatt gcttatttta aatcttacaa
    49921 tagtaccgat atctctactt ctcctgctat caaaatcaaa aacaatttcc tctaaatgat
    49981 ttatttcata atctttgaca cttaccatat ctctatatac taatttattt atagctatga
    50041 atagtgtaaa aatccccaaa ataaaaaatg aaacttttat aaacttcttc atttttagtc
    50101 tccttatatt ttgtgctatt gtgctataat attaattaaa attttaataa ttaaacttat
    50161 tcttttttga aatcttaata gaaaaataca ttgatattat aaaaattatt attgttaaaa
    50221 atgtcatact atataaattt atatacttag tattagctat atcagttaca taatttgaaa
    50281 gatatactcc tccaacttta ctataattaa aatgctttac ttgatataca atcatactaa
    50341 tcatcttcaa aagacttcca tatacaaagt aaattaaaat acttactata aaaccacaaa
    50401 ctttcatttt tccatatcca aacttaaaaa ttaaaggcag taaaatcgtc aaaattgcta
    50461 aaaatatatt tacagaaaaa aatacatcat ttaaaaccat atttctaaag aacactagtg
    50521 atgtcaattt acttagtaag gacacaatta ttgttacaga aattattagt cctacaccta
    50581 ataaatattt tgagtaaact atatcttcta tactaactgg catactcctt ataaaaatcc
    50641 ttgaatcatt ttcataatca cactcaaatg tatttataaa tatcaaataa ctaacaacaa
    50701 tacttactgt ataatatgaa attgaattaa ggaaaaaata aaacacaaaa aatagcaata
    50761 tgtactttaa attacggata ccatctgaac taattcctat tattaaatct tttcttatta
    50821 aattgaacat cctctcacct catttacata ataatacatt agttcttcta tagtaggatt
    50881 tttgacctta tgcttatcaa tttctatcaa taggcttttt actttcaagc tatctccaac
    50941 tttaacaagt gcctcattat aatatttata ctctttaaca ccaactatac tttctttaat
    51001 tgcttctaac tctgctttat ttgccctaat tatcttatat tcttgttcta ttaattcttt
    51061 cttaacagta aaaatctgat ttcctctatt tataaaaaca agataatcag caattttaat
    51121 taaattatca gtattatgag tagaaattat tacactacta cttccatttt tcacaaaatc
    51181 ttgtaagtac tgcattattt cagttcttac aatcgggtct ataccagcag ttggttcatc
    51241 caaaatcaat aacttagcat tatgactaag tgcgcttgat aacataagct tctcagattg
    51301 acctttagat aaattttcaa tatgcatatt ttcttctatg tcaaacttat taaggtattc
    51361 atcaaatttt tctctatcaa aattcttata aaataaagaa actatttttc taaattcctt
    51421 aacctttaaa tgtggataaa attgtaaaga atcatataca aaaccaatat tgtttttaaa
    51481 gcttattgga gaattttcaa tagtttcatt acaaagttca atttcacctt catctgcatc
    51541 aataatcccc attataattt ttattaaagt cgtttttccc gaaccatttg gtcctataat
    51601 cccaacaata tccccttcct ttaactctaa gtttatatta tttaacttga attttcctaa
    51661 tttttttgtg atattcctta tgctaaacac atcttcacct cttatgcatt tttttatacc
    51721 cacctataca tattttaaca cattcactac aaaggtcatt tataaattta taaattaaga
    51781 attataaaac tatgttataa tacaaagtat gaagaggtga atatatgaaa agaattataa
    51841 ctataaatac aaaccaatta aataagtccc ttgagactaa agaactctta actagaaaac
    51901 ttatcaatgc tggctttgaa gtgtattcag atatttatcc agacacagag cttattatat
    51961 caattggtgg tgatggctct tttttaagaa cagttagaga tttcgatttt cctgagattc
    52021 ccataatggg aataaataca ggtcatctgg ggttttttcc tgatatttta ccagataaga
    52081 ttgatagctt tattgaagct tatacaaaaa aagattatat tattcaagaa atgtcacttc
    52141 ttaatgcgga agtatataca actactagtg gttctaatat gcttgctgta aatgaggttg
    52201 ttataagagg agacaagtct aggactatac acctaaattt aagccttgat aataaacata
    52261 tacaaaattt tagtggagat ggtatgatta tttctacttc cacaggttca acagcctata
    52321 actattctgc tggaggtagt attgttgata taaatcttga acttatgcag attacacctt
    52381 tacatccgat aaataccaat gcatataggt gctttacatc aagtataata tgttcaaatg
    52441 aatctgttat aaagattgca ccagaatata gatttgaaga ttctgtattg attgtcgttg
    52501 atggagttga gcacagattt agacaaatcg aaaatataaa agttagtata tctgatgcaa
    52561 aaataaaatt acttagaatg tcaaactatg agttttggca tagagtatct gaaaaatttt
    52621 tatagactgt acttaggagg atttttaata tgaaaatagg aataatgagt gatactcatg
    52681 gtagcttact ttactttgaa aaagcgttaa atgtattatc tgattgtgat gttttattac
    52741 atggtggaga tgtcttatat catggaccta gaaatgatat tccagaagga tataatccta
    52801 aaaaatttat tgagacatta aataaattag aaaacattgt gattgtaaaa gggaactgtg
    52861 atgcagatgt tgaccagatg gttatagaac atcctattca gagtccttat gtcatgagcc
    52921 aatttggtga aattagaatt atattgaatc atggttatat agaatcagaa gaagaaatta
    52981 ttgacaaagc aaagaaaatg ggtggtgaca tattagtttt aggacatact catgtaaaaa
    53041 aactatacat ggatgataat ttaatagtaa taaatccagg aagtacttct atcccaaaag
    53101 atggaagcca ttctgtcgca attatagata tcataaaaac tgatgatgag gatgagcttg
    53161 aacttgatat aaacttaatt gacatcaata ctggaaatat aataaatatc aatgattagt
    53221 aaatttttaa taaaaataaa ataagcttta atttattaaa ctcataataa aaacaagtaa
    53281 accattttat ttctatctca attagaagtt taagtacaat accttaaact tctaattaaa
    53341 tagaatctgg tttacttgtt ttattatatg ctatattgaa atataactta gtttacttgt
    53401 ttctattata tattatattg gagtata
//
LOCUS       DAMKPG010000018        48795 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMKPG010000018 DAMKPG010000000
VERSION     DAMKPG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48795
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.020"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            115..1332
                     /locus_tag="PC621_001138"
     CDS             115..1332
                     /locus_tag="PC621_001138"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF4108309.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1427..1807
                     /locus_tag="PC621_001139"
     CDS             1427..1807
                     /locus_tag="PC621_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF4108310.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2091..2573
                     /locus_tag="PC621_001140"
     CDS             2091..2573
                     /locus_tag="PC621_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4108311.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2594..3409
                     /locus_tag="PC621_001141"
     CDS             2594..3409
                     /locus_tag="PC621_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4108312.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3460..5010
                     /locus_tag="PC621_001142"
     CDS             3460..5010
                     /locus_tag="PC621_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4108313.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5107..6459
                     /locus_tag="PC621_001143"
     CDS             5107..6459
                     /locus_tag="PC621_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF4108314.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6590..7348
                     /locus_tag="PC621_001144"
     CDS             6590..7348
                     /locus_tag="PC621_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108315.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7395..8114
                     /locus_tag="PC621_001145"
     CDS             7395..8114
                     /locus_tag="PC621_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF4108316.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8238..9638)
                     /locus_tag="PC621_001146"
     CDS             complement(8238..9638)
                     /locus_tag="PC621_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF4108317.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9642..10550)
                     /locus_tag="PC621_001147"
     CDS             complement(9642..10550)
                     /locus_tag="PC621_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4108318.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10811..11458
                     /locus_tag="PC621_001148"
     CDS             10811..11458
                     /locus_tag="PC621_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF4108319.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11783..14422
                     /locus_tag="PC621_001149"
     CDS             11783..14422
                     /locus_tag="PC621_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF4108320.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14480..15583)
                     /locus_tag="PC621_001150"
     CDS             complement(14480..15583)
                     /locus_tag="PC621_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF4108321.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCDEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15944..16765
                     /locus_tag="PC621_001151"
     CDS             15944..16765
                     /locus_tag="PC621_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF4108322.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16844..17055
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17142..18458
                     /gene="argH"
                     /locus_tag="PC621_001152"
     CDS             17142..18458
                     /gene="argH"
                     /locus_tag="PC621_001152"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF4108323.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18707..19045
                     /locus_tag="PC621_001153"
     CDS             18707..19045
                     /locus_tag="PC621_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF4108324.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19172..20461)
                     /locus_tag="PC621_001154"
     CDS             complement(19172..20461)
                     /locus_tag="PC621_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF4108325.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20541..21245
                     /locus_tag="PC621_001155"
     CDS             20541..21245
                     /locus_tag="PC621_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HDF4108326.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21337..22383
                     /gene="selD"
                     /locus_tag="PC621_001156"
     CDS             21337..22383
                     /gene="selD"
                     /locus_tag="PC621_001156"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21385..21387,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF4108327.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22401..23813
                     /locus_tag="PC621_001157"
     CDS             22401..23813
                     /locus_tag="PC621_001157"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF4108328.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            23927..25810
                     /gene="selB"
                     /locus_tag="PC621_001158"
     CDS             23927..25810
                     /gene="selB"
                     /locus_tag="PC621_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF4108329.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            25915..28653
                     /locus_tag="PC621_001159"
     CDS             25915..28653
                     /locus_tag="PC621_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF4108330.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28772..29716)
                     /gene="manA"
                     /locus_tag="PC621_001160"
     CDS             complement(28772..29716)
                     /gene="manA"
                     /locus_tag="PC621_001160"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF4108331.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29889..31250
                     /locus_tag="PC621_001161"
     CDS             29889..31250
                     /locus_tag="PC621_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4108332.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31338..33227
                     /locus_tag="PC621_001162"
     CDS             31338..33227
                     /locus_tag="PC621_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4108333.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33242..33697
                     /locus_tag="PC621_001163"
     CDS             33242..33697
                     /locus_tag="PC621_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4108334.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33756..34073
                     /locus_tag="PC621_001164"
     CDS             33756..34073
                     /locus_tag="PC621_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF4108335.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34098..35360
                     /locus_tag="PC621_001165"
     CDS             34098..35360
                     /locus_tag="PC621_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF4108336.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35465..36529
                     /locus_tag="PC621_001166"
     CDS             35465..36529
                     /locus_tag="PC621_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4108337.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKIGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKIIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36583..37761
                     /locus_tag="PC621_001167"
     CDS             36583..37761
                     /locus_tag="PC621_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF4108338.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(37952..39010)
                     /locus_tag="PC621_001168"
     CDS             complement(37952..39010)
                     /locus_tag="PC621_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF4108339.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39077..39448
                     /locus_tag="PC621_001169"
     CDS             39077..39448
                     /locus_tag="PC621_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF4108340.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39485..40159
                     /locus_tag="PC621_001170"
     CDS             39485..40159
                     /locus_tag="PC621_001170"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF4108341.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40408..41247
                     /locus_tag="PC621_001171"
     CDS             40408..41247
                     /locus_tag="PC621_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF4108342.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41490..41586
                     /locus_tag="PC621_001172"
     tRNA            41490..41586
                     /locus_tag="PC621_001172"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41524..41526,aa:Sec,seq:tca)
     gene            41737..42444
                     /locus_tag="PC621_001173"
     CDS             41737..42444
                     /locus_tag="PC621_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF4108343.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42586..43422)
                     /locus_tag="PC621_001174"
     CDS             complement(42586..43422)
                     /locus_tag="PC621_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4108344.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHIIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43610..44515
                     /locus_tag="PC621_001175"
     CDS             43610..44515
                     /locus_tag="PC621_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF4108345.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44524..45708
                     /locus_tag="PC621_001176"
     CDS             44524..45708
                     /locus_tag="PC621_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4108346.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            45823..47124
                     /locus_tag="PC621_001177"
     CDS             45823..47124
                     /locus_tag="PC621_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF4108347.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            47078..48121
                     /gene="holA"
                     /locus_tag="PC621_001178"
     CDS             47078..48121
                     /gene="holA"
                     /locus_tag="PC621_001178"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF4108348.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(48308..48574)
                     /gene="rpsT"
                     /locus_tag="PC621_001179"
     CDS             complement(48308..48574)
                     /gene="rpsT"
                     /locus_tag="PC621_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF4108349.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT        19144 a         5272 c         8338 g        16041 t
ORIGIN      
        1 tgtgattaat taaaaaatta taaaaatcgt ttacatgttg atttttaaaa aaaaaagata
       61 tagaattata tagaaagtaa taaacaaaaa ttggatttac gggagtggaa tgaaatgatg
      121 aatgttacct taacagatgt gaaagaggct agagagacca ttaagaacat agtaaaaaga
      181 actgacttac ttgaaagtgt taaacttagc gaaaaaacag gagcaaatgt gttctataaa
      241 tgtgagaatc ttcaaaaaac aggttctttt aaattaagag gagcttgtaa caaaatagcg
      301 agtttaacag atgaagaaaa ggcaaatgga gttatagctt caagtgcagg gaatcatgct
      361 caaggagttg cattgggagc aaaaatgact ggtataaaat ctacaatagt aatgccagca
      421 actgcgcctt tagcaaaagt tagtgcaact aaaggttatg gagcagaagt agttttaaat
      481 ggtgcggttt atgatgatgc atatgcaaaa gctgttgaaa tacaaaaaga aacaggagca
      541 actttcttac atccattcaa tgataagtat gttatagcag gacaaggaac tatatctctt
      601 gaaattttcg aacaattaga caataaagtt gatactatac tatgcccagt tggtggagga
      661 ggaatcatct ctggtgttgc tgttgcagca aaagcattaa atcctaatgt aaaaatagtc
      721 ggtgttcaaa ctgcaaatat accttcaatg aaagagtcaa taaaaaatgg aaaagtaaca
      781 acagcattta atgatactac aatagctgat ggtatagctg ttaaaactcc aggagattta
      841 acttttgaaa taataaatga attggttgat gaaatagtag tggttgaaga gacagaaatt
      901 gcagaatcaa tattatttat gatggaaagt caaaaaatag tttcagaagg tgctggtgct
      961 gtatgtacag ctgctatatt aagtggtaag tatgtaccag caaaagatga aaatgtagtt
     1021 tgtataatat ctggtggtaa tatagacata aatacgttat atagaataat aggtgttgct
     1081 ttagctaaag aaggaagaag atattcattt agtacaatta tggaagataa gccaggaaac
     1141 tttgctgagt taactagaat aataagtgag aatggtggaa acattttaag tgctaatcaa
     1201 ggtaaattat cagcagggga agctctagga aaacaaagtg cggaatttat attagagaca
     1261 atagattatg accatatagc tagaataaag aaagctatag aagaaaaagg ttttaaaatt
     1321 atagaattat aataaaaagt gtatataata tacaagaaaa atagtataat agtgtatgaa
     1381 gatacatttt atgtgtatta gattaacgaa ttaggaggat tttaaaatga aacatgaagt
     1441 aatacatact aatgatgcac cagcagcttt aggaccatat tctcaagcta taaaagctgg
     1501 aaacttatta tttgtatctg gacaagttcc tcttgtacca gagactatgg aagtagttga
     1561 aggagatgta caagctcaaa ctgctcaatc tttaaaaaat cttaaagcta tattagctga
     1621 aagtggagct gatttttcaa atgtagttaa gactacagta tttataaaag atatgaatga
     1681 atttggggct ataaacgaag tttatgctga atattttgga gaaaataaac cagcaagagc
     1741 ttgtgtagaa gttgctagat taccaaaaga cgttaaagtt gaaatagaag taatagctgt
     1801 attataaaaa tacaatattt ttaatttgaa aaggctgaaa aactatattc tagtgattca
     1861 gtctttttat aaaaaaataa atcacaataa agaacatgat aaatattgtc gaattaaaaa
     1921 ataaaattaa aataaatatt gcaatatttt tgagaaggtt ttataatgag tatataattt
     1981 gaaacgtgta actattaaat taggcatgag ttcttggata tttttatcct gaggattcat
     2041 gccttttttg tattgaaatt caaaaatcta tttaataagg aggaagtatt gtgttaaatt
     2101 ttttcaagaa aaataaaagc tataagttac atgcagtagt gagtggtaac agcattaata
     2161 ttgagaaagt aaacgattct gtgttttcaa aaaagttaat gggagatgga gttgcaatta
     2221 ttcctaatag taatgttgtt gtagcccctt gtaatggtaa agtaactgtg cttacagaat
     2281 caaagcatgc ctttggtatg gtctcagatg agggtgttga aatattagtc cacataggaa
     2341 ttgatacagt taatttacag ggtgaggggt ttaaaaatga agtgtctcaa ggagatactg
     2401 ttaaaaaagg tagtccaata ataagttttg aaagagaaaa aataaattct cagggaattg
     2461 attgtacaac aataataatt gtattaaatc atagcgaatt ttcagaaata aattgcatgg
     2521 tagaaaatga agttgttgca gggcaagata ctgttattga aattatgaaa taataggaag
     2581 ggattaattc aatatgagag tagtaaaaat ctttaataat aatgcattat ctacagtaac
     2641 taatgatagg aaagaagcta ttttactagg tcttggtata ggttttaata aacgtccagg
     2701 agataaagtt aatgaagata aaatagaaaa gatttattat gttcaagacc acatgcaaac
     2761 aaaattttta gaattattaa aaaatgttag tccagaagtg atggatgctt cacaacaaat
     2821 tataagtctt ataccaggaa atgagggaaa atttaataat aaagggatat tatctttagt
     2881 ggaacatatt agttttgcaa tagatagaat gaaaaataat gtttttttac ctaatttaat
     2941 gcttttggat ataaagatga tgtattcaaa agagtttgaa ttaggagtaa aagctttaga
     3001 aattatatat agagtatgtc atatccattt gcctgaagat gaagcaggtt acatagcttt
     3061 acattttgtc aatttacaat caaatgataa tttggcatat gatactttaa aatttgtaaa
     3121 aggtagtatt gatttgatta aagaatgtta tggattagaa ttagacgaat cgagtttaag
     3181 tacattacgt tttagaactc atcttaaatt tcttgctcaa agaattttta agaatgaaat
     3241 ttgtcaggat gataaaatga ttgaaatgta tgattattta attaacaatc atcctaaaaa
     3301 taaagaatat ttagaaaagt taaatgtata tattgaaaag gagtttaggt ataaattaga
     3361 taaacctgaa aaaatttatc tactactaca cctaacaaaa atcttataag aatgagatga
     3421 attagtaata gaaatatata attttagggg gggataagat tgaaagaaaa actacttgga
     3481 ggactggaaa catttgcaaa ggcaatggtt cagccattaa tgtatttatc gagtgcagga
     3541 atattaatgg tggtaggtgt actacttaca aatgatatgt tattaggagt acttccattt
     3601 tttaaatggg agccaattca aatatttgga agcctaattt ataactgtat aatggctatt
     3661 attaataatc ttgctgttat atttgctata ggtattcctg ctgcactagc aaaaaaagag
     3721 aagcacaaag cagcgttaat aggatttatg agttatataa tataccttac agcttccaac
     3781 actatgttaa catcactagg tcaactagca aaagaaagca agatgcttgg actgataggg
     3841 actggacaag ctactatttt aggtattcaa gttgtagata caagtgtatt tggtggaatt
     3901 attttagggt gtatagctgg atatgtattt aatcgtacaa gcaacaaaac atttaaagga
     3961 gctttacaga tatactcggg agcaaatttt agttttatat gcatgttctt tgtatccatt
     4021 ttaattggag ttgtgagtaa ctttatatgg ccattagctc aacaaggaat agcttctctt
     4081 gcaaatgtca tcaaagatac aggtacattt ggtttattct tatatggttt tttagagaga
     4141 atattagtac caactggact tcatcattta atctatacac catttcagtt ttctgattta
     4201 ggaggagtgc ttaaagttgg tgcagataca gtttcaggag cctatgctat agttatgaca
     4261 gaaatgaatt taccagttga gcgtttctca gatagtatat actacatggc aactggattt
     4321 acaaagatgt ttggttatat aggtatagga gctgccttta tttatactgc atataagcaa
     4381 aataagaaga aagttaaggc aacgattatt ccactaataa ttacggcagt attagcctct
     4441 gtaacagaac cattagactt tatgtttgta ttctgtgcac cacttttatt ctttgtacat
     4501 gcagttatag ctggtctttt tatagcttta ttaaaaatat ttgatgttac tgcattatgt
     4561 ggagggaact tattagtatc aattataatg aatactacag taggtgtaga aaaaacacac
     4621 tggccagtaa tgatgatatt aggacttgca caaattattg tatattttgt agtatttagt
     4681 tttttaatta agaaatttaa ttataaaact ccaggtcgtg aagatgagag tacagctaca
     4741 ggcgaagaac aatcaaaaca acttaattta gatgcaggta tagaaaatat aattgatggg
     4801 ttaggcggaa aagaaaatat taatacagta gaaaattgca tcactagatt acgtgttaat
     4861 gtaaaagatg aaagtaagat taatgaagat ataattaatt taacgccaaa tagcgggatt
     4921 gtaaggaaag gaaaagatat acaaataatt tttggacttc atgtgcatga ggtaagacgt
     4981 gcagtagaag attttttaga agcttattaa agatatatat ttatatagca agggtaaata
     5041 acagctttaa gtgctgttta ctatttatta aagatacata taatttatag aggaggaata
     5101 attattatga ttattactat tgttggtgga ggaagcacat ttactccagg tattgttaag
     5161 tcaattgcgt taagagaaaa agagttagag cttgaggaaa ttcgcttatt tgacattgat
     5221 aaagaacgcc aagataaagt agctgtagtt gtcaactgga tactaaaaga agatgttaaa
     5281 acagatgtta aactaactgt aactaattgt gaaaaagaag cgtatacaga tgctacattt
     5341 atatttgcac aaatgcgtgt tggtaaatat gaaatgcgtg agcaggatga aaaaattcca
     5401 ttgaaatatg gttgtgtagg tcaagaaact tgtggttgtg gagggatggc atacggactg
     5461 cgtacaatac aaccaatgat aaaggttatt gatgatgtag agaaatatgc taaaaaagat
     5521 tattggatat taaattactc taatcctgct gcaatcgttt ctgaagcttg tcgtaagtta
     5581 agaccaaatg caaggattat aaatatctgt gatatgccaa tagctattat agatatgata
     5641 gcggagtcat taaatatcaa tgacgttcat aatatacgct atgattattt tggtctaaat
     5701 cattttggct ggtttacttc aatagattat aaacatagag acttaatgga agagattaag
     5761 gaatacataa aagaaaataa aatattatta ccaaaatctt atatagaaaa cttaaataaa
     5821 agtcgtagta caggaaatcg ccatgctgta agtagctgga tacatgtttg ggaatctgtt
     5881 tatacaatgt tagaaagctt cccaaaatat ctgccaaaca cttatttaac atactatctt
     5941 ttaagtaaag aaacagtgga acattcaaac ataaatcgta ctcgtgcaaa tgaggttatg
     6001 gaaagccgtg aaaaagactt atttgaaggt gtgagacatt acttagaaac tggagaaatt
     6061 tctgaaaaag tattttatgc aggttcacat ggagattgga ttagtgattt ggcagtttca
     6121 attaaaaatg atacacgtag tcgtttttta gttataactg aaaatcgtgg agctatacca
     6181 aacatgccat atgatgcaat ggtagaaatt ccagcatata ttggtaaaga tggtccagaa
     6241 gttattgctc gaggtccaat acctctattc caacaaggat taatgatgca acaacttaac
     6301 agtgaaaaat tgatagtaga agcttatata gaaaatagtt atgaaaaagc tttacaagca
     6361 ttcacattaa ataaaactat tccaagtatg aatgttgcta aagaaatttt ggatgaaatg
     6421 atagaagtta ataaggaata ctggccagaa ttaagatagt aggataaaag aaagggtttt
     6481 tatttaataa ttagatatgt tttcagagtc ttttatcaaa atgtattcac aaacatatgg
     6541 caatatatta aaataaagag tgatattttt agaagaatgg ggtgatgcaa tgaacattat
     6601 aaataaaatt ttttggcaag aagataggat tggcatgatt acaaataatt tagaggcaga
     6661 tacacatagc cactgcatgt tacaactgtt cttagggata gaagacagca ttgaaataac
     6721 agtcaatgaa aaattagtta aatgtaattg tatcatagta gataaaaata tctctcattc
     6781 attttcagca agaaaaaaag tttattactc tgctattatt gaacctacat ctatatatgc
     6841 agaacagctt acctcaaaaa tgaatgattt tggctactgg atatgtgaca atgatggatt
     6901 agagaaatta cgtcaacaag gaactttttt gatatataac tctagtaaag aacaatattt
     6961 aagatttatg gaaatgctta ataactattt gaatatacct ataactctca aacattatga
     7021 tgacagaatt actgaattgc tcaatttatt acgtacttgt aattgtgata atcatactat
     7081 ttcaagtttt gcagataaag tgtccctatc agcaagtaga ttatcacatc tgtttaaaga
     7141 acaaataggg atacctttaa aaagctatat cttatttcat caaatggagt gtgcttttag
     7201 agaattactt tctgggaaaa atgttaccga agcttctatg actgctggat tcgatactcc
     7261 atcacacttt gcagggacag ttaagcgaat gatgggaatg cctgtttctt tatctttgaa
     7321 agatagcgaa tttttgaaag tatattaaat attctcgtta tataatgaag ttgtactaag
     7381 aaaggggctt tgttatgaat aaagaatatt tagaggaaac aaaaatgtta aattataatg
     7441 aaccacaact taagcttttg gtatcatcaa agaattggtt ggaattagat gattttcata
     7501 aaataaaatc tatttatgag tttgtacaga atgatatttt atttggatat aatgcttctg
     7561 atatgttaag cgcaacacag gtattaaatg atggtatggg acaatgcaat acaaaagcta
     7621 ctcttttaat ggcattatta cgtgcagtga atatcccttg taggcttcat gcttttgatg
     7681 taacaaaaga ttttcaacga ggtgctacat ccaaattgat ttcactatta gcaccaaaat
     7741 atattcttca tacttgggta gaggtatttt atcaagatag gtggattgct ctggagggtg
     7801 tcataactga taaaaaatat ttagaagcaa ttcagaagaa gttttttaat catggtggaa
     7861 cgtttaaaaa gtatgcaata gctacaaatg atttgaaaaa tacatctatt gattgggatg
     7921 gtaaagatac ttttatccaa aaagaagcta tcgtgtatga ttatggtatt tttccatcac
     7981 cagatgtatt tttttctact cattcacaac atatgtcaaa attaaaaaat ttcatatatg
     8041 tacatcttat cagaaaaata atgacaaaaa atgtatgtaa ggctagaaac aattatattg
     8101 ataaaaacga atgagtgttt aagaggtaac tatataggaa atatatagaa taattaaaac
     8161 tgtactggtt aaaatgaata aaaaggagtt atcatagagc aaacttaaat tgttcatagg
     8221 ataactcctt ttctgtatta acttctaata atttgatgtc tttttataaa acatatagat
     8281 acaattaaac cagtaaatgc aagagtagct gctattgaaa atgcaacatc tattccatga
     8341 atgttagaca gcatgtttga actcatatga gctttcttag tagcacttgt cattatagta
     8401 ataagtatag cagaacctat agctccagat atctgacgga gtgtgttatt aattgctgtg
     8461 gcatgagata taagttctct atctaatgtt ttaattcccc atgtagttaa tggcattaaa
     8521 accattgaaa taccaatcat acgaatacaa tacatcaatg acacatgaat taaagatgta
     8581 tctctgccta aaaaagcgaa ggataaagtt cctaaaaaca aacatcctgt tcctaatatt
     8641 gatagagcat gaggtccata tttatctaaa tgacgtcctg caattggatt gagtacaacc
     8701 atcaatatag caccaggtaa cattaatgaa cctgaagata atgcagaaaa tcctcttaca
     8761 gattgaacgt acagaggaat cattaatgta gcagacatca ttgaggcata tacaatcata
     8821 attaatattg tagaaataag aaatggttta tttttaaaaa ccctcaattc taaaaaaggt
     8881 tctttagatt ttaattgacg caatgtaaat aaaattaatg acataattcc tattaatatt
     8941 ggtaaatatg tggcaatatt agtccaccca taattacctt gattagtaaa tccaattaat
     9001 aaaccaccaa aacctagaga tgatagtact attgaaggta tatctaactt tagtttttga
     9061 gtttccccaa catttcttaa tagaataaat gatagtataa catctaatat agcaataggg
     9121 gaaagaaaca gaaataaact atgccaacca aatgaatcta ctaaccatcc tgataaagtt
     9181 ggacctattg ctggagcaaa tcctactgta ataccaacta aactcatagc tgcaccacgt
     9241 ttttcctcag ggcataaata gagtataata acttgtaaaa gtggcattag agaaccagaa
     9301 cccattgcct gcaacacacg agcaatcaac atagttgaaa aattattaga aaaaagtgca
     9361 ataatacatc ctatagaaaa gatagacatt gatgtaataa ataactttct agtactaaat
     9421 cgatttatta aataagctgt tgtgggtaca ataatacctg caataagtag ataaattgtt
     9481 gttaaccact gaccaatatc agcagtaatt ttaaaatctg acattatatt aggtaatgct
     9541 gttgacaata gtgtttgact gagagaactt ataaaagcac cagtcattac aattgctatt
     9601 attaaatttc cttttttatt gttactttgg gtttccataa tttataacgt ctcctttttt
     9661 aattgatttt taatgctgtt caataaaagt acttttggtc ttaaagtgtc ttctttttct
     9721 aaatatgcaa tcattgcatc cataattaaa gaatcatatt gttgattttt tccctcgacg
     9781 tataattttt ttattgcttc tagtaattta ggattttcaa ggagagaagt atcagttttg
     9841 attagttctt ctatatcttg tatcgtttta aaaaatagcg atttattatg aagggaaaag
     9901 taaacttcat cttcttgttt atgtaaaaac attgattttt caattaaagg atttagatta
     9961 tccatatttt ctacaactaa atctagaatt tgtatagtaa atttggcaat aaagtataca
    10021 tttatttctc ttttttgtac atgaataatg tatataaact gatgaattag agcatccaat
    10081 aagaaaacta aatcaaatat aattttttct attttatcac catagttatc aaataagctt
    10141 aatttgtatt cattcaataa gttagctcgt gattttgttt gcaatttact tgtagcctta
    10201 cgttgctctt cagaaaaatt ttctcttata taggtattta tggtagttct agaaaaaata
    10261 tttttccata cagcaattat ctttctgttt aacttttctg atgatggaat gtcattattt
    10321 tcgtttatgg aatgtataat ttctaaaaac ttttcatcat aataattgat tatttcgcag
    10381 ataaaacttt ccttagtttc aaaatgcttg taaaaagtag ctttagacat tttacactct
    10441 tttacaacat ccgatattga agtattttga aaaccttttt ggtcaaaaat tttttgtgca
    10501 atatttataa tgagcctttg tttttctgtc actttattat ctcctttcac gcaaaaacgt
    10561 ttagtactat atagtaccta caagttttaa taaatatatt atacatcatt tggaaaaaaa
    10621 atcaatatat agactgtaat atgaaatttt tgaaaaattt gttgttattt ttagtcgtat
    10681 aaaaaagaaa cttaagagtt tcttaagaat tgtttaaaga agttgcaata aattagtggt
    10741 atgattatag tatgaaagag aatgtcttga aatttttctg tatcaaattc caattatgga
    10801 ggtgtagtat atgaaaaaga aaatttgtgt agcaataatt tttgctctat ttatagtttt
    10861 tgtagaaatg tttattgata gctctagcga aggaagtaaa attatgacag ctgaattaag
    10921 taaatacagg aaaggcgtaa atgtattatc aataaacaat attatagtag cgaataaaaa
    10981 atatagtttg cccaaagatt attctcctca ggaaagcagt gaagcgagag atgcttttta
    11041 taaaatgaat aaagatgctc aaaagtcagg attaaattta aaagctttta gtacatatag
    11101 aagttatgaa tatcaagata gattatttaa atcatatgta aaagaacatg gagaaaaaga
    11161 agcaaataga ttttcagcta aacctggaga gagtgagcat caaactggac ttgcttttga
    11221 tattggagga gatgaccagt cttgttgggc aaataaaaag ttcaacaata caaaagaagc
    11281 taagtggttg tatgaaaatg catataaata tggttttata cttagatatc cagaaggtaa
    11341 agagaatata acaggatata tgtatgaatc ttggcactat agatatgttg gaactgaaca
    11401 tagcaaaaac tttgcaatga ataatttgac tttagaagaa tatttacata taaattaaga
    11461 gatggtttgt taaaattagg ttaaaataaa aaataaccta gactattagc tgtgtaaagt
    11521 gatataatgt aaatacaatt taagataact actcgaaggg gagtagctta cacgaaagtg
    11581 gtaattaatc gtcaagacag tagaaatact cggttaatta gcaagggatt ccttgatagc
    11641 aagaccttca aaataagcat taggtatgtt tgttttgggg tcttttttta ttcaaggaat
    11701 ttttatttaa aaaaatttaa atttcaagta tacataagag cccccaaaca taaaataaat
    11761 ttattgaaga ggagggcaac aaatgtctta caaaagtact tatcaagaag taattaaaaa
    11821 tttagactca agtttatctg gtttgagtaa agaaagagca gaagagttgt tggaaaaaaa
    11881 tggagctaat gaattaaaag aagcagataa ggttcctact tataagcttt tcctagaaag
    11941 ttttaaagac ccattagtaa taattctatt gatagctgct ttagtacaaa tatttttagg
    12001 tgaaacagtg gagtctataa taatttttgc agttattatt ataaactctg ttctgggtgt
    12061 tgtacaaaca aaaaaagcag aaagttcatt agaaagtttg aaaaatttat ctgcaccaaa
    12121 cgcaaaggta ataagagaca ataaaaaaat gacaattcca gcaaaaaatc tagtagttgg
    12181 agatattgta ttcttagaag ctggcgatta cataccagca gatggaaggc ttatagaagc
    12241 acaatctctc aaggttgtag aagggatgtt aactggagaa tcagaaccag ttttaaagca
    12301 tacagaaaaa ataagtgaag atgtaagttt aggtgaccag aaaaatatgg tatttagtgg
    12361 tgctgtagtt gtttatggta gaggttcttt tgtagtgact ggaacaggta tggatactga
    12421 gatgggtaaa atagctgggc ttttagaaag tgctgaaaat aaacttacac cattgcaaca
    12481 aaaaatagag gattttagta aaaaacttgg tgtaggtatt gtaatacttg ctcttttaat
    12541 atttataatt caagtagcaa gaaattactt tatggctgga aatggagaaa tgacacaaat
    12601 tattttagat tcattcatgt tctctgtagc agtagcagta gcagctatac cagaagctct
    12661 atcatcaata gtcactatag tattggctgt tggaacaaat agtatggcaa aaaaacaagc
    12721 cataataaga aaattaccag cagtagaaac tttaggttca acaagtgtaa tatgtactga
    12781 taaaacaggt actcttacac aaaataaaat gacagtagtt gattattata tgtatggaac
    12841 taataatgat gagttagata aagaaaaagt aaactattct tatcatgcta aattattgac
    12901 tatggtttct acattgtgta atgactctca tataactagt gatggtaaag aagttggtga
    12961 cccaacagaa gtggctttaa taaattattc aagtaaaaat gatttagatt atgataaatt
    13021 aagaagtaag tatatcagaa taaatgaaat accatttgat tcagacagaa agcttatgtc
    13081 tactgtaaat agtatatatg gagattatat aatgtttaca aaaggtgctc cagatattgt
    13141 gtttagtaga tgtaaatatg ctttgaaaaa tggttcaaaa gtagatataa cagatgagat
    13201 aatagaagaa tataaacata aaaatgaaga gttttcaaat agagcactta gagtattagc
    13261 ttttgcaata aaagatgtcc cagaagaaga ttttatacct tcaattgatg acgaacatga
    13321 catgacattg gttggaataa tggcaatgat agacccacca agagaagaag taatggatgc
    13381 agtaaaagag gcaaagagtg ctggtataaa aactgttatg ataactggtg accataaaac
    13441 aacagcagca gcaattgcaa aagagattgg tataatggaa gaaggagact tggctcttac
    13501 tggaaaagag cttgatgctt tgagtgaaga acaactatat gaaaaactag aaaatataag
    13561 tgtatatgcc agagtctcgc cagaaaataa aattcgtata gtaaaagcat ggcaacataa
    13621 gaataatata acagcaatga ctggtgatgg tgttaatgat gcacctgcat taaaacaagc
    13681 agatatagga attggtatgg gaagtggtac tgatgtagca aaagatgctt ctgctatggt
    13741 attggttgat gataactttg caagtatagt aaatgctgtt gaagttggta gaacagtata
    13801 cagtaatata ataaaatcaa ttacttatct atttgcagga aacttaggag ctatagtatc
    13861 catagtattt gcagtgtttg caggttggtc aaatccattt acagctttac aacttttgtt
    13921 tataaactta gtaaatgact ctttaccagc aattgcatta gggtttgaaa agtcagaaaa
    13981 aaatataatg aataaacctc ctagacaccc ggatgaaagt atactagcag gaggaacatt
    14041 gaaggcagtt gctataagag gttgtataat aggtgtagtt acaataattg cacaatatat
    14101 aggattacaa gtatctccag aacttggagt agcaatggca ttttcaacat tgatattagc
    14161 tagaataatg caaacattac ctgcacgttc taataatcaa acagtaataa aattaggttt
    14221 tttcagcaat aaatacgtta taggagcagt tttagtctgt cttgggcttt actctttaac
    14281 actaatacca gtgctaagag gaatattctc tataccaaca acgtttggat tgtaccagtt
    14341 ggggatttgc ctagggttag cattaatttc atcaatatta atggaactta acaaattaat
    14401 aaatagaagt gaagtaaaat aatcaaaaaa ttaaagggca tattgatttt aacaatatgc
    14461 cttttataaa tagtctaatt tattctatat tatcgattat ttggaatgct tttataactt
    14521 caggtaattc tttttcttca ggcattctaa atcttatgaa atttttatca agtccagtaa
    14581 aatcacttcc agatataact cttattttta atttagcaaa ctcttgttgt aaatctatgt
    14641 ccttgttttt atgttcaact gtcataattg atacagtatc actagtttca gctatattaa
    14701 ggtttttcca tggaattagt aattgatttt ttatatcttt tgttttttct tttaattctt
    14761 ctataaattg aacatcatct aataaatttg ctgcaatact acgagatatt tcagacactt
    14821 cgtaaggagt agaaatcttt ttaatagggc taacaagttg ttctggaaga actgcatatc
    14881 ctgctcttag tcctgctaaa ccaaatcctt ttgaaaatgt ttttaaagca attacattat
    14941 cataattatt tagaagcttg acagctgaat tttcttttgg catgtattca ccataagctt
    15001 catctaccat aactgctata tcatatttag ctgcttcctt tactatattt tcaattgatg
    15061 ataaaggtat aatttgtcct gtaggattgt ttggattgtc tatgtaaatt actttatatt
    15121 ctggatttat tttttcaata aattcttttt cattaaactt gaaattatcc tctttcttta
    15181 aaagaacata atcataagtt gcaccatgca tttttatatc tgtctcatat tctgagaact
    15241 gaggtgaata tcctaaaact ttatctccct tttcaataaa tagtctattt aaaaggtaaa
    15301 taacatgtat agaaccatct gccaaacaaa ttctgtcagt atctaatgct ataaaatctt
    15361 tccaataatc gactatatta tcttttaaag caattgaatg aggatatcct cttatcatat
    15421 caaattttaa agcattaaaa gcttctacag ctttgcttga gtaagacacg gtatttatac
    15481 cttcatcaca gtctatctcg aatacatctt ctgaatgtcc agattctatt gcataacttt
    15541 ttttcaactg catttcaata gctttgttaa tttttagttt catactttat cactccttat
    15601 ttagtcaata ataaatgtta taaaagttgc tagatttaaa atattatgaa aatatataat
    15661 agtaaaaaat gaatataaat attataatag ctcactttat ttaatatgat tatatatcat
    15721 atattgtaaa atttctagca ttataagact ataaattgaa aatattcaga aaaatactac
    15781 aagaaacgat ttattttgct cttaaaataa gttaaatgaa tgttattaat tttgtaaatt
    15841 gaaatagttt aagtataaag ttatgatata atctaataag ggtaattctt tagattatat
    15901 taaggaaata taggtataca ataaaaagtt atggagttgg attatgggat ataaattaat
    15961 tgtaacagat atggatggaa ctttattagg caataatcat aaagttacag atgaaaataa
    16021 aactgcacta caaaaagtta taaaaagcgg tattaatgta actttagcaa caggaagagc
    16081 cttcgattca gctaaatgca atgtagattt tcttaaagag gacatgccaa taatagcttg
    16141 taatggttca ttaataagag agcaaaatgg aaatataata tattcaaata agattgatac
    16201 aagaacatgt ttaaacattt tagatgtatt agataaatac gatatttact accaatgtaa
    16261 tagtatagat tctatgcttt cgaaaaaaat agaaggacgt gaagatagat taagtgtatt
    16321 tttaggctct gaaacagaag taattgtaaa agatgatttg agagaagaaa tctttaaaaa
    16381 agatatacta aaatttgtaa tcatagaaga aaaaaatcca tctattttgg atgaaataag
    16441 aaaagaattg agaaaagttc aaggaataaa gataacttct tcatggccaa acaatattga
    16501 agtcatgaat gaaggtgttg ataaaggtaa tgcagttaaa attttagctg aaaaaatgaa
    16561 tatagacaga gaagatataa ttgcatttgg agacaattac aatgacatag aaatgataaa
    16621 gtttgctggt cttggggtag ctatgggaaa tgcagaagaa ttgattaaac aagaagctga
    16681 ttatgttaca gatacaaatc aagacagtgg agttgcaaag gcaatttata aattcctaga
    16741 gttaaaagaa tcacttagct cttaaaacag tatataatag gaaaaaatag gttgactatt
    16801 ttgtagatag taattataat acaaatagat aaaacataaa aatttagatt cagtgaaaaa
    16861 gagagtagat atagagaaaa tctcagcgaa ttgggggatg gtgtgagcct aatgataaca
    16921 ctttatattg aaaagaactt tggagaatac tacttgaaat tcagtatggt agtaggtaat
    16981 ctcgccttaa gagaatagag tgggtaaaca ttgtttacca actagagtgg taacacggaa
    17041 tatttcgtct cttggtcttt ttgaccaaga gattttttag tttcattgag ttacaagact
    17101 tataattaaa atacaaaaaa ttaaaatatg aggtgaaagt tatgaaatta tggggcggac
    17161 gctttagaaa agcggaaaat caacttatgg aagaatttaa taaatctttt ggctatgatt
    17221 gtgtactgta caaaaaagat atcgaaggaa gtgtagcaca tgttcatatg caagtaaaat
    17281 gtggactttt aacagaagaa gaaggaaagt ctataacaga aggtttaaaa gggatacttg
    17341 aagatgtaga aaatggaaag ttagtattgg atggagagta tgaagatata catagtttta
    17401 ctgagataaa tttaattcaa agaattggag atgttggcaa gaagcttcat acagcacgta
    17461 gtagaaatga ccaagttgca gttgatatga gattgtatgc taaagaaaaa gcaaatgatt
    17521 tggtgggttt aatatctgaa tttaaagcta ctataaaaga tgttgctgac aaaaacccag
    17581 tgatgatgcc aggctatact catcttcaaa gagctcaagt agtgacattt aagcatcact
    17641 taatggcata ttatagtatg tttgatagag atgagaaaag aataaaaaat gctatagaaa
    17701 tattagatga atcaccttta ggctgtggag ctttagcagg aactacgcat gatatagata
    17761 gaagtataac ttgtgaacaa ttaggattta aaaaagtagt agataatttt atggatggag
    17821 taagtgatag agattattta ctagagctaa tgagtgattt ttcaattata atgatgcact
    17881 taagtagatt aagtgaagag ttgatacttt ggagttctca agaatttgga tttgttgaga
    17941 tagatgattt atatacaact ggaagtagta taatgcctca gaagaaaaat ccagatggtg
    18001 cagagcttat aagaggtaag actggcagag tatatggaaa cttatttggt ctttttacag
    18061 taatgaaagg aataccatta gcatataata aagacatgca agaagataaa gaaggttttt
    18121 tcgatagtgt acatactctt gaaatgtgta tacaaataat ggatagaatg atagccacac
    18181 taaaggtaaa tgaggataaa atgaaacaag cagtaaaaaa tggtttttta aatgcaacag
    18241 aagttgcgga ttatttggtt aaaaataatg ttgcttttag agatgcacat ggtatagtag
    18301 gaagtatcgt tatatattgt gaagataata aaaaagcaat agaagattta acattagaag
    18361 aattacataa gttttcagat gcttttaagg aagatattta tgattttatc gactatgaga
    18421 gtatattaaa taagggaata aaaaagaatt taaaataatg tatttatttt taaaagctgt
    18481 aaatttgaat gttatcattt aaatttacag ctttttattg tgtaagattt tattatataa
    18541 aagtaagaaa cttaaaagga gtagtattta aaataaatac aaataaaaaa ttattattaa
    18601 aatgataatt aatatcaata agtattgaca aaattatttc taaatactat tatatataat
    18661 ataaataata atgaaaatca atatcaaata aatgggagga aatctaatga acttattagt
    18721 gattggtggt catgaaagaa tggaaaaaga ttatatggtt atggccaaga aaaaaggtta
    18781 taagacaaag gtatatacta ccatgtcatc taaattaaat aattctatag gaagaccaga
    18841 tgcagttgtc atacttacat caactgtttc acataagatg tctagaacgg ttgagtctca
    18901 agctaaaaaa caagatatat taatcgtaag gcataaaaat agtagtaagg tagcatttaa
    18961 tgaatgtcta gatatggttg atgaatgtct gggaaattgt tcaaaatgta agcttaaaga
    19021 caaacaaaaa caaaaaaata tataggtata aaaaatacat aaacataaaa ttaaaagtac
    19081 tgagataata gttataatta ttatctcagt acttttagat ttataaggat aagtatatta
    19141 taaatttttt attataattt ccaactactt gttacttttt catatttttt ttacttctag
    19201 ctttttttct ttttttcttc tttatatttg aagctattat aaataaaact aaaagcacta
    19261 gaatacctgc aataatctta acaaaagtca ttaaaatatt atttccagta aaaaatggaa
    19321 gactgctatc aagattattt tttgcaatta agtctatact gttttctagt ttaccattat
    19381 tatatacatt taaagtacca accttagctc ctttttttat aggcaaatca attttattta
    19441 actctgtttt ggtatcataa ttgccttgag attcatcttt ttctaagaat atcctataac
    19501 tattttttgg ttcataaatc aattcatgtt gttttgtaaa caatactttt ttagtatctg
    19561 tgtatttttc tttatcaaca atagtagcac acttaaagtt atcaaatcca taatcaatca
    19621 gaatacgtga gtctaaatat aaatcagcat tagttgaatt aaatacagca acgataactc
    19681 ttctgccatc ttttacagca ctcgataaaa gacatttacc agcagcatca gtatatccag
    19741 tttttatacc atcaactata tcatatttta tatttatagg ttgacctttg tatgtaatct
    19801 tatcatgtga tgtcaaaaac ttattagtgt ttacaaagta tctctcataa ggatatgctt
    19861 ttgtagcttc aaatttaagt gacttagttt ttacaatttg tctaaaagta tcattattca
    19921 tagcttctct agctatgaga gccatatcat gagctgttgt gtagtgattt gggtctggta
    19981 atccatgagg attattaaaa tgagtattag tagctccaat tttttttgcc tcagagttca
    20041 tgaggtcaat aaatttttgt acattgccac caccaacata tctagccaat acaaaagcag
    20101 catcattagc agaatgaacc aaaagagcat ctagtaactg ctttacagta aaagattccc
    20161 cattttttag atacatacta gagccatcta tttgaggtaa atctttaatt tctattactt
    20221 gatttaaatc tttaacctcc tttagaacta aacaagctgt ccatacttta gttgttgaag
    20281 ctggataaag cttagaattt ccatccttat tatatagtat tttgccagac tcatagtcca
    20341 ttaaaactgc atgtttagca actatagctg gttcattatc tgcaaaagag atattagagc
    20401 ttcctaaaaa tgaagtaaat atcaataaac agattaataa aagtgataaa tttcttttca
    20461 ttgtgagcct ccaaatttta aattcatctt ttagtatagc atatatttgt caataattaa
    20521 aagaaaggag tgattaatat atgaaaaaaa aggtaaaatt tatttcgttt tttgttttat
    20581 ttattttaat aatatcatca ataatcgttt ataaatcgct tattttcaaa gatgatattt
    20641 atgttgtaag taaaaaaaat ttacctaatg aaaaaacaca tgttgtagag aatgaaatta
    20701 aaggtgaaga taataatcac aataaagtaa ctaaagacat agataaaaat tttcaaaata
    20761 ataaaataac tatatacata agtggagcag taaatagacc tggaattgta actatagagt
    20821 cagacaagag actttatgat gctgtagaat tactaggagg aacgacaaaa gaagcagatt
    20881 taaatggagt caatctatca gtaagattag aagatgaaca acattacata atacctaaaa
    20941 taggtgaagc aacatctgtt acaagtaatg atgattccaa taaaccaaat cagaagaatg
    21001 agtcaagagg tgagcctaaa aataaatcca ataaaaatat atctaacgaa tcaaaagtaa
    21061 atataaatgt agcgactata gaagagttag attctctacc tggggttgga gaagctacag
    21121 ccaataaaat tttacaacac agagaagaaa atggacagtt tagttcaatt gaggaaataa
    21181 aaaatgtaaa tggtattgga gataaaaagt atgaaaatat aaaagattta atttgtgtag
    21241 actaaaatat aatacaaaac ttacatatag atatgttatt ataaatatgt atgtatatgt
    21301 gtgtacatgt ggaaatttag taaaggaggc acttttatga aaaaagaaca aaaaaaactt
    21361 acagagatga caacagcagg tggttgagcc gctaaaatag ggccagaggt tctggcatca
    21421 gttttatctc aattgcctaa aaatgataat atagaaaatt tattagtagg actagataca
    21481 gcagatgatg ctgcagttta taaattaaat gatgatatgg cacttatcca aactttagac
    21541 ttctttacac caatggtaga taacccatac gtatttggtc aaattgctgc atcaaattca
    21601 ctatcagatg tatatgctat gggaggcaaa cctttggttg ctatgaatat tgtttgtttt
    21661 ccatcatgcc atgatatgga tgtacttgcg gaaatattaa aaggtgggtt tgataaagtg
    21721 aaagaaagtg gagcactttt ggttggaggg catactgtag atgacaaaga gcctaaatat
    21781 ggtctttcag tttctgggat tgtaagtcca aataaagttt tatcaaatgc tactgcaaaa
    21841 cctggtgata agttaattat aacaaaacca attggagtgg gtgtattaaa tacagccatg
    21901 aaagaaggta tggtagaaca acatatagca gataaagtaa tagaaataat gatacattta
    21961 aataaatatg cagcaatgag ttttgagaaa tttgatgtaa attcggttac agatataact
    22021 ggttttggtc tacttggtca tactttagaa atggccaaag cttctgaagt aagtattgaa
    22081 attgaatcaa aacatgtacc aattattgaa ggtgcaatag aaatggcaca aatgggaatt
    22141 atacctgctg gtatgtataa aaatatgcat tatgtatcta aagatgtaga agttgtagga
    22201 aatatagaag ttgcagttca agatatttta tacgacccac aaacatcagg agggttgtta
    22261 atttcagtaa aagaagaact tgcagaggaa cttgtaaaag atatgaaatt aaatggagca
    22321 atagaagcta aaattatagg ttctgtagtt ccaaaagggg aaaaatatat tacagtttta
    22381 taaaatatta tagagaaaga gtgattgttt tgagcaaaag agaattgttt gcaatgttgc
    22441 cttcagttga tgaagtatta agtgataata gaattgtaga aataataaat gaatatccta
    22501 gaagtttagt tttagaatct gtgagagaag taatagattt aaaaagacaa tttatattaa
    22561 gattaaaaga agatgcatca aactctgtaa caatagagtt tggtgaaata atagaatctg
    22621 caatagagag agttaagtta aattactcat tatctttaaa aaaagttata aatgcaactg
    22681 gtacagttat tcatacaaat ttagggaggt ctttacttag tgaagatata aaagatgaat
    22741 tatggtgtgc tgcttcaagg tattcaaact tagaatacga tttggataat ggagaaagag
    22801 gttcaagata ctctcattta actagtacaa taaaaagact tactggagca gaagatgtac
    22861 tagttgtaaa taataatgca gcggcagtac ttcttgtact tagcacaatg gctaaaggag
    22921 gagaagcaat tgtatctcgt ggagaattag ttgaagtagg tggttcattt aggattccaa
    22981 gtataatggc tcttagtggt gcagaattag ttgaagttgg ttcaactaat aaaactcatt
    23041 tgaaagatta caaggaggcc attacagaag atacaaatgt attgatgaag gttcatacaa
    23101 gtaattatag aataatggga ttcacagaga gtgtttctat agaagaattg gttaatttag
    23161 gtaaaaaata taaactacct gtaatagaag acttaggaag tggagttttt atagatttat
    23221 caaaatacgg tttatcttat gaaccaactg tattggattc tattcgacaa ggtgcagatg
    23281 tagtaacttt tagtggagat aagatgcttg gaggtccaca agcaggtatt atagttggga
    23341 aaaaagagta tatagaaaaa atgaagaaaa atcaacttac tagagcactt agagttgata
    23401 agcttacaat atgtgctctt gaagcaacat tgagaatgta tttagatgag acaaaagcta
    23461 tagaaaatat acctacttta aaaatgttga catataaaat tgaagaatta gaagtaaaag
    23521 caaataaact atttgaaaaa ataactgctt taaacttaaa tgcaaacata aatatagaag
    23581 atggattttc acaagtggga ggagggtcta tgcctcttga aactataagt acaaaggtaa
    23641 tatcaataac tcctgaacat atgaatgttt catctttaga aaaaaaacta agacttagtg
    23701 aagcacatat aatagccaga gtgtatgata ataaatatgt actagatgta agaactatat
    23761 ttgatgatga atttgatgta atagttgcgg aattgagaaa agcatttaat taaataaatt
    23821 tgagctaata aatattaaat ttagaagtat aagtatgaag ttttgattaa aaaatagcta
    23881 cacacacttg tgactttagt catagcaggt ttagaggggg caaaaaatga aaaatgtagt
    23941 aattggaaca gcaggtcata ttgaccacgg aaaaactact cttataaaag cattgactgg
    24001 aagagagact gatacattag atgaagaaaa aaagagaggt atttcaataa acttaggatt
    24061 tacttatttt gatttaccaa acaatagaag agctggaatt gttgatgtcc caggacacga
    24121 aaaattcata aaaaacatgt tagctggagc atcaggaatt gatatagttt tacttgttgt
    24181 agcagcagat gaaggggtta tgccacagac agttgagcat ttagatattc taagcttttt
    24241 aaatatcaaa aatggaatag tggttctaac taagtctgat actgtagatg atgaatttag
    24301 agagctagta aaagaagata tttgtgaaaa aataaaagga acttttcttg aagatgctga
    24361 aataattgaa gttgattcta tatctaaaag aggtatagac actcttattg aaaaaataga
    24421 taaaatgagt gatgatatag aagataaaaa tgaaaactct ccagcaagac ttaatataga
    24481 cagagtattt tcaataaaag ggtttggtac tgtaataaca ggcactttaa tagaaggaaa
    24541 gataagtata gaagatgatt tagttatata tccacaggta ttaaaaacaa aaataagaag
    24601 tatacaagtt catggagaaa ataaagatac agcttatgca ggtcaaagaa cagcaatcaa
    24661 tatatcaaat gtaaaagtgg atgatataaa aagaggagat gttttagctg caccaaactc
    24721 attagaggaa tcaatgatgt tagatgtaaa attatcattg gtaaatcatt tagataaagg
    24781 attaaagcac tgggatagat taagattgta tcatggaact agagagatac tttgtagagc
    24841 agttccacta gataaagaat taattgaaag tggagaaagt ggctatgttc agttgagact
    24901 tgaagaaagc atagtatcta agaaaggtga tacgtttgta gtgagacgtt attctccaat
    24961 ggaaactata ggtggaggtg taataattga cccatctcct aaaaaacata agaaatttga
    25021 tgaaaaagtt atagaagcat taaaaataaa agaaaaaggc gaactaaaag atataataga
    25081 agagtactta aaaaggaatt taaaaaatta tccaaatata aaagaaataa tgagctatag
    25141 tggtgctcat gaagaagatg taaaaagagc gcttgaagca cttatatcag aagataaagt
    25201 atttattata ggaaatatgt atatgcatat aaatcaatac aataaattaa aagaaaatac
    25261 aataaagctt ttaagtgagt accataagaa gtataggctt agaaaaggga tactaaaaga
    25321 agaagtaagg tctaagatag agagtaattt taagactagg gagatggata ttttattaga
    25381 aaaattgtct acagaaaatg ctataaaaat agaaaataat atagtatctc tattagattt
    25441 tgaggttatt ttaaatgata agcaaaaaga aatagcaaaa aaaatagaaa aaagattaaa
    25501 atcatgtgga gtttcttcta tactgacaat agatgaagtt tctgaaggaa atcataacta
    25561 tgctgaagta ttggagtcaa tgataggtaa taaagtagag aaattagatg atttgtatat
    25621 aatggacaaa gatatttatg aaaatgcaaa gaacattttg ataaattata taaaagaaaa
    25681 taaagaaata acattaggag aatacagaga tttgattgat tctagcagaa agaattgcat
    25741 gattatattg gaaaacttcg atagaaacaa aataactaaa agagtagaaa acaaaagaat
    25801 tttgttttag cagaaaatgt ctgtttttgc tttaaggtat aaagaaggaa ttataatgtc
    25861 gagtatcgta taattaatta gtataaaata ttaattaaga ggaagtgtaa agtattgaag
    25921 aatataatac ctgatgtaaa accagatata aaagaaagag aagacatgag ccttttattt
    25981 ataataatgg gctcagtact tttattattt attataaaag atatagagaa atatgaaact
    26041 ttattacatg ccatatatac gatattttta gtagctacag gagtaattat atttaatact
    26101 acaaaattta aagtagattc attttctaca tttttaggat taatttttat agctactgga
    26161 gtattggagt gtatatattt atttaattcc ttggggatta aaactaagtc tataatggag
    26221 attaacatca caatatcagc aataacagat ttatttccta tactgggtgt atacttaagt
    26281 tttaaatttg ttaaggataa caaacaaatt tattcaagta taattttatt tatagcaact
    26341 gctacattaa caataacatc tttattcata atatcaggaa tttgtgacta tctgggaaat
    26401 ggtaaatcac aatatgcttt aggagttata atatctatat tcataataat tatttctatc
    26461 attagtggaa ttgaattaaa ttcaagttca aataaaggta aatgggaata tgatgaaaaa
    26521 aagattttta acagaataat agtaataata attttatcaa gagttccaaa tctattgcat
    26581 atagttattg ataatagaaa tgtagagcgg atattatcac agataataat aaatgtagcc
    26641 ttatattatt tatataatta catagtatct aagaatatta agaaaacagc actggaattg
    26701 catgatacga atgaagaatt aacaaaaaaa acggaaagtt taaaagaaaa gaataaaaaa
    26761 ttaatctatg agacagaaaa gatagaggaa cttaagggaa tacttatgaa aagagaatct
    26821 aggctacaat ctacattaga tgtagcagta aatagcatag tagtgtttag caaagatgga
    26881 gaaattacat atgcaaataa gagctttaga aatacttttg gagactataa agaacaagat
    26941 aaattgactg ataaaataaa aaacttcaat gatttgatag aaagcattca ttatgtgttt
    27001 gttcatgaga agaatgcaga gaaacttatt agtacaagcg ataataaagt ttatcaagct
    27061 atatttgcac ctttaattat tgcttcacaa acagaaggag tattgtgtgt actagaagat
    27121 aagactaaga aaaaggagta cgaacgcaaa cttattgaag caaataagag aagtaaagat
    27181 tttttagaaa gtattggaga tggcatagta gtgcttgaag gtaataacaa aatatatgtt
    27241 aataatgcat gtagggaaat ttttaaaaat aatttagaga gtattgattt ttcattagta
    27301 tgtaaaagtg aagaaagtat agaaaaaaga tatgtaatag atggtgaagt aaagtatgta
    27361 gagatgagtt tttctcagta tactaatgct ggtactaata aaactattat tgtaatacgt
    27421 gatacaacct ctcgaaaaat atctcaaata aaacttgagg aaagtcaatc atcatattca
    27481 agatttatag atatattacc tgatggcata tgcttattaa aagaagattt aagtataaac
    27541 tatgcaaata agtcgttttt agatatgcta gcttttacaa acatagatga tataaaagat
    27601 tctaatatta aactattaat gaatgcgaat actgaagaaa aaatgaaatt tacggataat
    27661 atggctaaag ttttaaatga aaatagaagt atacttttac tggaatatga attgataaat
    27721 agttatgatg atatagtaga agtagaatta agtgcattac cttttgctat atacaatact
    27781 agatacataa tgcttattat aaaagattta gtacataaga aatattccga gcaagcagaa
    27841 aaagaactgc ttgaaagatt taaaacagat aaaatcaaaa cggagttttt tgcaaatatg
    27901 tctcatgaat taaaaactcc actaaatgtt atatctagtt caaatcaact ggtagattct
    27961 ttctatagaa atgaaaagat aaaagattac aataacaata ttaaatctca tgtggattta
    28021 gttagacaaa gttcttatag gcttcaaaga ttaataaata acattataga tttaacaaag
    28081 atggagtctg ggttctatac actaaaactt gcgaaataca atattgtatc tgtaatagaa
    28141 gacttattta tgaatataga agagtatgca cttagaaaag acataaaaat tctttttgat
    28201 acagaccttg aagaaattaa tgtttatata gacaaggttg aaattgagag aattatgttg
    28261 aatctattgt caaactgtat taagtttact gataatggag gctggatata tgtaagtatt
    28321 cattataaaa tagataaagt aatcataagt gtaaaagaca ctggagttgg aataccacaa
    28381 gataaattag aattgatttt tgaagaattt tcacaagttg ataagacttt atccagaaat
    28441 actgaaggaa gtggaatagg gcttgctata gttaagaatc tagtaagtct tcatggtgga
    28501 gatatagaag tagtaagtga agtgaataaa ggcacagagt tcttaatttc acttccaatg
    28561 aagggattta gtagtgaaca ttacacagaa gataagagaa tatataatat tcaagaaaaa
    28621 ataaaaatag aattttctga tatatactat taaaaatgat tatgagcaaa aataaaataa
    28681 aaatactatt tgtttaaata tttaagttgt aagaaatagg ctatcataag gtaaattatt
    28741 atgatagcct atttttagct aggttttata tctatatgtg agaacatata atctctaaat
    28801 taccttcaaa actaaaatct ccaaagtcgt ttggcagtat aaaatgcata ccctttttta
    28861 aattatattc tacagaattg tgtataagtt ttccatttcc atccaacaca ctgtataaag
    28921 aaaaaggtgt attgtgtgta aaattacatt ttccaaaaac atctaattta taaacactaa
    28981 aaaattcgtt tgacacaaat gttgtgcatt taaagtctga tgttgatacg attttatagt
    29041 cagtatcgaa atttatatga ggaacattcg taacatctat tgatttttga acatgaagtt
    29101 cacgcttatt acctgaattg tcagttctat cataatcata gactctataa gtagtatctg
    29161 agttttgttg agtttctaaa atcaatgttc ctttgcatat agcatgtata gtaccacttg
    29221 gaacatagaa aaagtctccc ttttttatat taacctttct aagtagtttg tcccattcat
    29281 tattatttac catatttaca aattctttat gagattttgc attatgacct attataattt
    29341 ctgcatcatc agaacaatct attacatacc aacactctgt cttacctaac tctccatttt
    29401 catttttctt agcatattca tcatttggat ggacttgaac tgataaatta tcatttgcat
    29461 ccaatatttt tgtaagtaaa ggaaacttat caccaggtgt gtttccaaat aattctctat
    29521 ttttattcca caattcagat agttttttac ctttatattt tccattttct attaagcaat
    29581 ctccattttt atgagagctt atagcccaac attcaccagt tgttggcgaa tctatttcat
    29641 aattaaattt atctttaagt gcagtaccac cccatattct atccataaaa ataggtttta
    29701 aaaataatgg ttccataatc ctgttcctcc tatgatatta aatttatcct acaaataatt
    29761 atatataaat agattataaa atcaatagta aaaaagtttt gtcccttgaa atgacataaa
    29821 gtataaggag ttattaaata agataaacta tgatatataa tataaagttc taaattggag
    29881 tgataattat ggagttgttt aaggcagaaa gcaaaaaata tgaaaaagaa tttttaaaat
    29941 tattggagca atgggttagt ataccttcat tttatgatag aaaaactgta tctaaagata
    30001 tgccttttgg aaaaggagta tatgatgcat taaactggtt tgaaaatcta ggtagagaaa
    30061 acaatttcaa agtaaaaaat atagataatc atgctgttca aattgaatat ggaaatggta
    30121 aagaatatgt agatatattt ggacattgtg atgttgtaaa tccaggtgaa ggatgggata
    30181 gtgaaccatt taaactaaat attataggtg ataaattagt tgctagaggt gtatctgata
    30241 ataaaggacc tatgatagtg aattttttag cactaaaaat gataaaggat ttagatatta
    30301 atttaaaaag aaaggttaga ttaatagctg gaggaaatga agagagtgga tttaaatgta
    30361 taaagcatta ttatagcaaa gagccatatg gtgtatgtgg atttactcca gatgctaaat
    30421 ttcctgtatt aaatggagaa aaaggtggag caataataaa gttaatttca aatatagacg
    30481 ataaatcttt atatataagt ggtggaatag agtttaatac aataccagat aaagtttata
    30541 taaagaatgt agaaaaattg ggtaaagata atatatgttt tgatattaac aatatatcga
    30601 taaattatga taatggcaat tatattgtgt atggaaaagg tggtcattca tctaaacccg
    30661 aaaaatcaat aaatcctata ttagctacaa ttaaactttt gtcagaaaat attgatgaaa
    30721 aatggacaaa agatttatat aagttgataa atcaagataa tataaatgga aacttatttg
    30781 gtttaaatat tgaggggaaa tgtgggattt tatcaatagt gccaacaata ataaatatag
    30841 tagatggaaa attagaagta gttttaagcg ttagatatcc agaaatatta accattgaag
    30901 atataataaa aaaatttaat ttgtatatgg agcaaaataa tattaataaa tttgagctta
    30961 ttggggaaaa tttaaagcaa gcaaattata tagatagaaa ttcaaaactt gtaagaagtt
    31021 tgcatgatat atatataaag tattctggag atttgaaaaa tgatgttaga gtaactagtg
    31081 caggaagtta tgcttctgaa atgaataatt cagtaatatt tggttgtgaa tttcctgatg
    31141 gtagctttgg caatgtccat agtgcgaatg aatttgctag cttagataga tttataactg
    31201 caataggtat atatgcagag gcaataatta cattatgtaa taaaatataa atacaaaatg
    31261 tcccttatat agacatatta tatatacaat taaaatttta cttgatatat aattgaaatc
    31321 aaagagaagg tgatacaatg ataactaaga aacatgcaat aatagtaaag agtttaagca
    31381 ataaagatgg atatatgaca tcaaacgaat tagctatcaa attggatgtg tctactaaaa
    31441 ctattaaaag atatatagca gatttaaata gtgttttaag caaatatgat ttggtaatag
    31501 cttcttctag aggtatagga tacaagttaa gcggttctaa aaacaatata gccagagctg
    31561 ttaaagaagc aaataaatat attgatggtt ttttagatga ctctgaagag tcaagaatgt
    31621 caaatataat atgtatgctt attaatagaa attatatgag tatagaagca atggcagaag
    31681 aattaaatct aagtatagcc gctataaata aattgtctag taagcttaaa agaaaacttg
    31741 aaaagtatga cttagttata aaatctaaac cttactatgg aagctatata catggtgaag
    31801 aaattaatat aaggcaatta attactgact atgcaataaa attagatgaa aaaagtaaag
    31861 tgaaggtgtt tttagatgac attagtgaaa acgatatata ctgtatagaa agtattttag
    31921 aaaagcactt aagagaaaaa gatacaataa tttcagataa agattttaat cttttgcttt
    31981 caaaaataat tgtttctgta tttaggagta agagaggtca tagtaacaat ataaacttaa
    32041 tggatacaag ttatagattt cataactact actttataga aaatctaatg aaagaaatat
    32101 ctgataaaat tggatttaaa cttattgaag atgaagtaat ttatatttca aattattctg
    32161 gtgtaatagc ttataaaggt acacaaggaa gaaaaaacac aagtgaaatt gaggaacgaa
    32221 taagtagtgt tataagtagt gcacttcaag atatattcct tatttcaggt agtgattata
    32281 caaaagatga tgagtttatg gttgcaatct ctgaccatat aaagagattt ttaaatagag
    32341 ctagagctaa tgtaaaatca aataatcctt tacttcatca aataaaagag aagtttccta
    32401 tagcttttaa tttagccgtg tttttatcaa ataaactaga aactgagttt aatttaaaac
    32461 ttgatgaaga tgagcttgga tatatagcta tccactttgc tgcttcaaat gaaagaatga
    32521 aaaaaaatac tagtaagaaa atttgtatag tttgtcatta tgggatagga acaggacaac
    32581 ttatttctga aaagctaaaa cagaatataa gtgatttaag tgttgtaggt gtttatccag
    32641 ttagatatct tgatatggct ataagtcaag atgttgattt gatagtatct accgttgaat
    32701 taaaaggata tgaaaaacct gtcttatata tagaaaatat atttgatgat agcttaattg
    32761 aaaacgtaaa caaagccttt tatgaaaagg aagaaagaag aaagataata agtaacatgt
    32821 ttgatgaaaa agcttttttc agtataaagg ctagtactaa agaagaggtc ataatttcac
    32881 tttcaaataa actaaaagaa agagatttta tagaggaaag ttctataaaa agtataatag
    32941 atagagaaaa tatttcatca actgaaatag gaaatctagt agcaatacct cacacaatag
    33001 tgaagggaga taaaaaatct ataataggag ttggaatact tgaaaatcca ataatttggg
    33061 ataagcaaga ggttcagcta gtatttatgg tattttttaa tactaaagaa aagcataatt
    33121 tctcgatatt taaatacctt tataatttta taaaagatga aggaggagta agaggaatta
    33181 ttaaaatatg tgactttaat aaattaatgg cactgatagg taattaagga ggaaattaat
    33241 aatgggaaca aacatattta acaaagaata tgtattttta aatgttgatg ctaagtcaaa
    33301 ggttgaagtt ttaaaattta tatcaaaaaa agctaaagac ttaaatttgg cagaagatga
    33361 gagcttagta tatgaaggtt taatggctag agaagaacaa tttactacaa atttaggaga
    33421 atctatagca atacctcaca ctaaaaatga tgcaattgaa aatccagcag tagtagtatt
    33481 gaagtttaat gaagatgttg tttggaatga aggtgaggat aaggttaaat tagcaataag
    33541 cttacttatg ccaggaaaaa gtaaagagaa tattcatctt aaattattat catctctttc
    33601 aagaaaatta ataaataaag aatttaaaga ttctctttta aaaagtgata atgttgaaga
    33661 aatatctaac ttaataaatg aagctttagg tttataatta aatactaagg ttataaaata
    33721 aaaaaataat taactaaaaa taggaggaat ttgaaatgaa taaaaaatta gtagcattat
    33781 gtgcttgccc aatgggacta gctcacactt tcatggcagc agaagcaata gagcaagcag
    33841 caaaggcatt aggttatgaa gcaaaagttg aaactcaagg tgctgatggt gtacaaaatg
    33901 agttaacaag agatgatata ttaggagcaa ctatgataat acatgcagta gcaataacac
    33961 cagaaggtat ggaaagattt gatggttgtg aagtttatga agttgaatta caagaagcta
    34021 taaaaaatgc agaaggtgtt ataaaagaaa tagaagaaga tttaggaata taataaattt
    34081 attgtggagg aaatagaatg gcaattaaga aaaaagttat aaacagttca ggtgcttcta
    34141 caacaggagg aaataatgta ggaaaatcta ctccatctag tccaactcaa tcaaagggaa
    34201 atggaaaatg gaaagaaata agtaaacata taatgacagg tatatcatat atgataccag
    34261 ttttagtaat gggtggactt ataggggcct tatctcaatt aataccatat gctatattag
    34321 gacttgaccc atctgtaggt atagttgatg ctatgaactc aggagaattt acagggttca
    34381 agcttagtct tttaaatata gcacaattaa tgtcaaattt cggatttact ttgtttggat
    34441 ttgcgatacc actgtttgca gcattttgtg caaactctat aggtggaaaa acagctttaa
    34501 ttgcaggatt tataggtgga tatatagcta ataaacctgt aggtgtacca caattcattg
    34561 atggacaatg gactgaagtt gttccagttg catcaggatt tttaggtgca atactaatag
    34621 cattcataat aggatatttt gtaaaatggt taaataaatc tataaaagtt tctcataact
    34681 ggttagcttt taaaacaaca tttttaatac cattaatagc gtcattagct tgtatggtat
    34741 tgatgatatt tataataact ccatttggtg gattaattaa tgaaagtatg aaaaacttct
    34801 taacagcagc aggagcagct ggagaatatg tatatgcaac agctttggca gcagctacag
    34861 cattcgactt aggaggacca ataaataaag cagcaggatt cgttgcactt ggtttaacta
    34921 cagagaatgt acttccaata acagcaagaa caatagctat agttgttcct ccaattggat
    34981 taggattaac aacattatta gataaaagat tagtaggaag aagagtatat gatagacaat
    35041 tctatcaagc aggaaaaact tctatattct tatcatttat gggtatatct gaaggagcaa
    35101 taccatttgc acttgagaga ccaggttttg ttataccttt aaacatagtt ggaagtgtaa
    35161 taggagctat tacaggtata attctaggag caattcaatg gttcccagag tcagctatat
    35221 gggcatggcc tctagtagat aacttatttg gatatataat aggtatagca gttggtgcta
    35281 tattcatagc agtaggaaat atattctata gaaataaatt aataaaagat ggtaaacttg
    35341 ttgtagatta tatcgactag tatttaattt ttactatcta taaataactt tggctggtaa
    35401 tatggtttta atcaatatta ccagccttac ctatatgatt aattaaaggc ggtggagata
    35461 agatatggat aagatagaaa aagtaagaga gtattttaga gaatatgata tagatggatt
    35521 tcttataaat agtcctacaa acaagtttta tgttggaaat ttatttagct cttcaggtta
    35581 tgtttttata acgaaagaat cacaatatat aatagtagat tttagatatt ttgaagaaat
    35641 taaaagaaaa tctagtttgt ttaatgtagt attaatggac aaaactagaa ctcattttga
    35701 tataattaat gatatttgta gagagcagaa tataaaggaa attggatttg agggaaatga
    35761 agtatctttt gatttatata gaagtatgag taataagtta agtgcaacct taaaatcagt
    35821 tgatttatct acattaaggg aaacaaaaaa tgaagacgaa ataaaatata taaaaaaagc
    35881 ttgtgaaata gttgatgcta cattttatca tatagtagac tttataaaga ttggtatgac
    35941 tgaaaagcaa gttgaaaatg aaattgttag aattataaaa gatttaggtg gtcaaaaaga
    36001 atctttcgat acaatagttg catctgggct tagaggagca ttacctcatg gaaaagcaag
    36061 tgaaaaaatt atagagtatg gagattttgt tacttttgac tttggtgcta agtataataa
    36121 ttattgttca gatattacca gaaccatttg tatgggtact ataaataaag agttagaaga
    36181 aatatataat atagtaagaa aagctaatga agagtgtata agagttttaa gaccgggaat
    36241 gactactgga gagatagata aagttgcaag agacataatt ggttcatatg gatatgccaa
    36301 taattttggt cataatctgg gtcatggtgt aggaattatg gttcatgaat atcctgcctt
    36361 agctcctgaa tcaaatgaag tattaaaaga aggaatgatt gtaactatag agcctggtat
    36421 atatgttcca agtcttggag gcgtaagaat agaagatgat gtactcataa cacaagatgg
    36481 atgtatgaga ttgactacat ctactaagga tttaatagtt gtaagctaaa aataaattag
    36541 ataaatattt tataacaata agaaaagaag aggtaaattt aaatgtataa ttttgatgat
    36601 aaaccagata gagtaagtga aaagtgtaga aaatgggatt taaatattat aagagataaa
    36661 tttggagata ttagagaaga ttttgtacca atgtggattg ctgatatgga ttttaaaata
    36721 ccaacagaaa tagaaaacaa atttattgaa gctgtaagaa gaggtgtatt tggatataca
    36781 tactgttatg atgaatttta tgattcagtt attggttggc aaaaaaatat gcatgaggta
    36841 aatgtagaaa aagaatggat tacattaact tatggaactg tatcaacact tcattatgta
    36901 gtacaagctt tttgtaagaa aggtgatagt ataatattaa acacaccagt ttatgacccc
    36961 tttgaatcat cagcaaaaaa acaaggtgta aatgtaattt gtaatacatt agatgttgta
    37021 aatgatagat attatataaa ttttgataaa ttagaagttc aaattaaaga aaataagcct
    37081 aaattaatga tgttttgtac accacataat ccatctggaa ggatatggac aattgaagaa
    37141 atgacaaggg tagcaactat atgtaaagaa aataatgtta ttttggttgc agatgaagta
    37201 catgcagagc atattcatta tggtaaattt aattcaatac ttaagattgg aaaagagctt
    37261 ttagaaaatg ttattctact gacatcacca aataagggct ttaatcttgg tggattaaag
    37321 acttcttatt caatagttat aaataagaat ataagggata aatttagaaa taaattaaag
    37381 caaaattcta taacttctcc aaatgtgttt ggaataatag gattgataac cgcttataat
    37441 gaatgtcatg agtggttaag tggtgtaaat gaatatataa aatcaaatta tgaactttta
    37501 gaaacttggg taaataaata taataagata aaattgatga aaatggaatc atcttactta
    37561 gcttggatgg atataagtgg attaggtata agtgcaagtg agtttacaga taaattggct
    37621 atagatacag gagtattatt agaagatggg agtcattttg taaaagatgg tgagaagtat
    37681 gttagaataa atttaggaac tcaaaaggaa aatgtaattg aagctttaaa tagaatggat
    37741 ttatttttaa agtctttata aaaatttggt tgaattagta ttttatataa atttaagcta
    37801 ttaatatggt atatacataa gtagaatttg ttttagatta cttaagtata taccatattt
    37861 tatataaaaa ttaaatttgc attagtcatg tgataactat ttaaattcat atgtattttg
    37921 tatgtataat agatataatt attatttaaa cttattgttt atttaatttt tcaatagaaa
    37981 aagatttatc tgtaagttta aaattatctt ttataccctt agttaaatat actttcttat
    38041 ccttagttat gaatatagca tctacattat caatttcatt aattaaatcc ataccttttt
    38101 caagaccaag tccaaatgta gaagtggata aggcatcaca attaatagat ttatcagaaa
    38161 ttattgtaac actacttaga ttgttttcaa aaggataccc tgtaaatgga tttaacatat
    38221 gatgatatat ttttccgtct ttttcgataa atctttcata aattccagaa gtaacaacag
    38281 atttatttgt ggtttctatg tttcctatgg attcaccatt tttactagtg gggtcttgaa
    38341 tgcctatttt aaaaggttta tcatttttac catcttctaa agtaaatata tttcctccta
    38401 agtttactaa accttttttt acgtcctctg attttaaata ggctacaatt ttatcagcag
    38461 catatccttt agctatggca cctaaatcaa ttttcatatt ttctttggtt aattttatag
    38521 atttattttt ttcatccaat atgatgttct tataatcaat aagaggcaga agtttttgta
    38581 tctcagaatc acttggaact ttagctttat ctgttccaat agcccataaa tttgataatg
    38641 gaccaattgt tatgtcaaaa tatccatctg ataatttgga atattgtata gcttctttta
    38701 cgacgaaaaa tgtatcatca gaaactctga caaatttttt accagcatta tcatttattt
    38761 tagaaacatc acttcctggt atatgagtgc tcattttgtt gtcaatatat cttaaaattg
    38821 aatcacattc atttaagatt ttatcacttt tggattcttt tacattaaat atagtaactt
    38881 cgtttactgt accaaggtag tagctagttt tactatattt agtattctgg cttgaactcc
    38941 taaaaaatga aaataaaatt aatgctaaaa tcaatatcac aaaaataaat gtgatttttt
    39001 tcttattcat ggtatattcc tcctatgaaa ttgtgttatt attttattat aacataattt
    39061 cataggagga caaaaaatga agaaaaaaga cattcttctt attttaggca ttttggttgt
    39121 tattatagca tgttatggga taataaatgt tataaattct aaaaatgctg gaaatattga
    39181 gatttatgta gataataaat tgtataaaac tgtttcaata aatgccaaag aagaatttaa
    39241 aatagagaat agaggggggt ataacatagt aaagattcat gataaagggg ttgagatagt
    39301 agatgcttca tgccctgata aggtttgcgt acatactgga tttataaaca agccaagtca
    39361 aagcatagtt tgtataccta atagagtgag cataaaaata aatacaaatg aaaagaatga
    39421 taatcaagaa gatataattt ctaaataaaa taataaaaat aattgaaagg aattggtgat
    39481 aattatggtt aacttaggaa acgattggga tgaattattg aaagaagaat ttgaaaaaga
    39541 ttactactta aacttaagaa aatttcttat agaagaatat aaaacacgcc aaattttccc
    39601 aaatatgcac aatatatatg aagctttaaa acacacttca tacaaagaca caaaagtctt
    39661 aatattagga caagaccctt atcatggaga taatcaagct catggtttag ctttttcagt
    39721 tcaaccacaa gtgaaaactc caccatctct attaaatatg tataaagaat taaaagatga
    39781 tttaggttgt tttataccta ataatggata tttaatgcct tgggcagacc aaggagtgtt
    39841 acttttaaat actgctctta ctgttagagc gcatgaagct aattctcata agaacaaagg
    39901 atgggaaatt ttcactgata gagtaatatc tattttaagt gagcgtgaag acccagttat
    39961 ttttgtacta tggggaagta atgcacgtaa aaaagtagag ttgattgata caagtaaaca
    40021 ttatattttg gaagcacctc atccaagccc attgtcagca agtaaaggct tttttggatg
    40081 taaacacttc tctaagataa atgaaatttt gaaaaagcta ggaaaagaac ctattaattg
    40141 gcagatagaa aatatataaa agtaaaaggt aacaaaatga taacaaaaca acaataaaca
    40201 atgacattgt tgtaactaat agaaatatgc ttaaatagtt gaaaatacta tgtatgcata
    40261 tttctatttt tttgtcaagt tgacaactct tgaaaaaata gttacaattt agttacacaa
    40321 aaaacacaaa taaaaaaaga aacttacaaa gcagaatttt atgaagcaaa atatatagtg
    40381 taaaaaattt tagggaggaa attttatatg aattttaaaa aattgagtaa tgctaaagtc
    40441 tcggtaatgg cagttgtttt atcagttgga atattatcgg gatactctgc gcttaataaa
    40501 caagtaacat tagtagtaag gggagaagaa agaactatat caacatttag ttctaatgta
    40561 ggagaccttt tagaatctca aaacattagc tatgatgcaa atgatatagt aactttagat
    40621 gaaaatacaa agttatctaa tggagataaa atagaagtaa ttgatgtaaa ggaaaaaaca
    40681 gttgtagaaa ctaaagacat tccatttgaa gttacagttg ttgaagatgg ctctttatta
    40741 aagggtgatt ctaaggttaa agaagaagga aagtctggta aaagtgaatt aatatataaa
    40801 gaaacttatc acaatggaaa gaaagttgaa aaggaatttg tgaaaaaggt tgttaaagct
    40861 gagccagtaa ataaggttgt aagcaaaggg acaaaggtag aaatgcaagt agcctcatca
    40921 aggggagaaa gttcaagaag aatggcttca tcatcatcta gtagcagtag tagtaacaat
    40981 ggtaaaaata tgagagtggt agcaactgca tatactggtc atagtataac tgcaacagga
    41041 acaaaaccta agtggggaac aatagctgta gaccctaagg ttataccata tggaactaaa
    41101 gtttatatac ctcaatttga catggtgttt actgcagaag actgtggtgg agcaataaaa
    41161 ggaaataaaa ttgatatcta catgaatgat tcaaaaacgg tttataactg gggaagaaaa
    41221 actatagata tacgtatatt aaaatagtta aataatttat atttttactg gaaattatat
    41281 tttgattata tagagaagaa actctttata gattcttaaa atataatttc cagtttttat
    41341 tttggatatg taataaaaca ggtaaatttt aaccatgtat agataaaagc tatttaaatt
    41401 ataatttact atcagataaa tctataaaaa ttgttgcaaa gagtcaaaat atatgatatt
    41461 atagatttcg ttgagaagga tttcattatg ggagtggatg ggtgccggtg tgccctctag
    41521 tcttcaaaac tagtatgagg ggttaagagc ctcttaggtg ggttcgattc ccacgcattc
    41581 ccgccaatat gcaaaaggta tcttttaatt aaaagatacc tttttaaatt tattttaaga
    41641 taatttattt ttaataatat ataatgtttt gtattatata atgtaaattt tctaatacta
    41701 ttatatattt agataaaaat ctaggagtgt ggaaagatgg aaaataattt tacaacgaga
    41761 actagttttc tagttggaga tgatgggata gaaaaactta ataattcaaa tataatagta
    41821 tttggagttg gaggagttgg tagttttact gttgaagctt tagcaagagc tggagtaggc
    41881 aatattacta ttgtagattt tgatgatgta gatataacca atataaatag acaaatacca
    41941 gccctacatt caactgtagg aaggtacaaa gttgatgtca tggaagaaag aatattagat
    42001 ataaatccta atatcaatat aaaaaaaata agaagtcttt ataataaaga tactagtgac
    42061 gaaatactaa ctgaaagata tgattatgtt gtggatgcga tagacatggt aagttccaaa
    42121 atacatctaa ttgaaacttg tgaaaaaaaa gggttgaaaa taataagttc tatgggtatg
    42181 ggaaataaat tagacccaac taaaatagtc gttacagata tacacaaaac aagtacttgt
    42241 cctctagcta aagttatgag gaaagaactt agggatagag gtatcaaaaa gttaaaagta
    42301 gtatattcta ctgaacaacc aatagaactt aagaagaaag taatgaatgg tagaaaagta
    42361 acacctggta gtgtatcatt tgtcccatct gtaggaggtt taataatcgc atctgtaata
    42421 gtaaatgaac tattaggaca ataagtttta aatattttaa tttataaatt ttatatacaa
    42481 atttgaaggc tagaagcatg aatataaagc aattttatat tcatactttt agcctaatta
    42541 ttttatatta ccttatttta tgtagttgta gttttatttt ttagattatg tatctatttc
    42601 ataattatat tttgtaaata taatttctcc atcactataa aaaacaatat ctcctattct
    42661 atctgttcta taaattgata catggttttg ttttagatta ttaataacct ccctatgtgg
    42721 atgaccatat tgatttttat atccacatga tattgcagct atatctggac taattttttt
    42781 tataaattct aaagatgaag aagaattact tccatgatga cctattttta taaaattaat
    42841 attatttaat ttaaaagaat gtaaaatttc tttttcattt tcttcttcag catcacccat
    42901 aaataaaaaa gaattatcgt taaaagttaa tttgaaaact atagaattta aattagattc
    42961 ttcttgtata tatgatggac ttaatacata tatatttata ttgttatcta tgtttaaaac
    43021 atcattttta tagagatgct gaatagataa gtttttatcc gtacaagaat ttattagatt
    43081 ttgataagcc tcactatcag ttgattgttc aggcatatat atggagttta cattaaattt
    43141 ttttataaca ttatcaagac ttcctatgtg gtctgaatct ggatgagttg ctattataat
    43201 atcaatagtt tttattcttt tttgtctaag ataactaata attatatttt cgctatcttc
    43261 atcacctcca tcaattaata tattcttatt tgtgggtgtt tgaactaaaa tactatcacc
    43321 ttgtccaaca tcaattatat gtatagatag tagagatttt ttgtcacagc cgattaaaag
    43381 tgatattata ataagtattg ataaaaaaat tttatttctc aataaaaaat cactccattc
    43441 attacatgtt tatataaatt tatgatgggt aatataagat atatatgact tattatattt
    43501 tatattttaa acaatataaa tgaaattaat accataataa atttggagtt ttttatatat
    43561 acaaaataaa atttttaaaa ttaagcaata aaaaaggtag gaggatttta tgtttttcaa
    43621 agatattgaa ctaaattcaa aaaaagagtt agacccatat tttgatttag tagattatga
    43681 agcttgtgag tactgtttta gtactttata tatgtggcaa catgtttata agacaggata
    43741 ttatataggt gaagattttg ccgttttagt tggggaatac gaaggagata gtttctcaat
    43801 attaccactt gctaaaaaag ataaacttcc agaagttgta gattttgtcc ttgaatattt
    43861 ttctaaaaat aataaaaaaa tatacttaag aggtattact actgaagtag tagagttttt
    43921 aaaagaaaaa tatccaggta gatttgagta tatagaagaa agagatttat ttgactatat
    43981 ttatgatgca gaaagtttaa gaactctagc tggtaaaaag aatcaaaaga agagaaacca
    44041 tataaactat ttcttaaaag agtatgcagg tagatatgaa gctaaattat tagataaaga
    44101 aaactttgac gagtgtttag tactaatgaa ggaatgggaa agtaataagg aagaaaataa
    44161 tgaatttgat gaaagtatgg atgacgaatt aataggtatc aaaaagatat ttaaccatta
    44221 tgacatatta aaagataagg taaaagtgtt tggtgtttat gtggatggaa aattagaggc
    44281 ttttagtata ggagaacttt taaatccaaa tatggcattg attcatatag aaaaagctaa
    44341 tcccgatata agaggtctgt atccatttat aaatcaacaa tttttggtaa gtgaatttaa
    44401 ggacgttgag tttgtaaata gagaagaaga tttaggtatt gaaggattaa gaaaagctaa
    44461 actttcttat catccttgca gatttgttga aaagtacagt gttagggagg cttaatagac
    44521 tatatggaga ttaggtatgc taaagaagaa gagatagaaa gtataaaaga gatttggagt
    44581 tattgtttta acgatacgga gagttttatg aagtactatt ttaatgataa atataaatct
    44641 gaaaatactg tagtagcttt agatgaagga aaaattattt cttctcttca gctaaatcag
    44701 tataagttat tactaaatag caaggtatat aatacatcat atgtagtagg agtttctaca
    44761 cttccagagg gaagaggagc tggatatatg aacaaagtaa tgaaatttac tttaaacgaa
    44821 ttgtataaaa aagggcaatt agtttctata ttgatgccta ttgactatag actttataga
    44881 agatttggat atgagcattg ttacgaccag attgaatata ctattaacac agatgattta
    44941 aaaaatttta agtcaagtgg aaaaatgata aagtctaatt tatcacaaat tgatgatttg
    45001 atacgaatag atagaacttt tttaaatgag gttaatggta atgtgttaaa agatgaacat
    45061 tattatgaaa acttatttaa agagattcaa agtgaagatg gatttttata cattcatgaa
    45121 ggaaatgaaa aagatggata tatagtttat tttcttcagg aagataaaat gtttgtcaga
    45181 gagttatttt ataaaaatat agatgccctt aaatcaatgc ttaaatttat atataatcac
    45241 aatactcaat gtaagatagt tactatatct actcctacaa tagataagat aagatttata
    45301 ttagacaacc ctaaagatag tgatataaaa ataaaacctt ttatgatggg aagagttata
    45361 aatgtaaaga agtttattga agatatagat attgagaagg atattaatag ctcatttaat
    45421 ttattaatag aagacaagtt tatagatgaa aataatggtc tatttaaaat ttctatacag
    45481 aataagaagg tatctgtaga gcagttagat aaaaaaggtg cagaaaagcc tcaagaagat
    45541 tttgatatta aattagatat aaatacctta acacagttat cattttctta tatagatgta
    45601 aatgaagcta ttttcttaaa tgatataaaa gatgtttcag aagaaacact agaaacactt
    45661 aattgtattt tttctaaaaa aaatacctat ataaatgaat atatataggt agaatacgtt
    45721 aaaagatttt aaaaatcttt tgacgtattt ttttgtttag ataacaattt atcttgttta
    45781 aaaattttct atttgaaaat aatctaaata atatactttt atatgggagt gattataatt
    45841 caaataagaa gaccattatt aattatatta atgtttgtgg tagctatatc atttatcatt
    45901 acaaataaca caaaaaacga cgaagaactt aatgataaga taataactat acaaggggtt
    45961 gtcaaaggaa aaattgaaaa gaaaagatac aatcagtaca aggtaggggt ttttctaata
    46021 aatgattata caaaaaataa aaatttgaag attggtcaga aagttaatat tacaggtaaa
    46081 ttcaaaagtt tagataaaat gaagtatgat gattttgatt atggaaggta tataaaaagt
    46141 actggatata aaggtatagt gtatataaat aactataaaa ttattgataa aaataaactt
    46201 tactctttaa taggggaaat aaagttttat attagtaaaa cttataggta cttatataag
    46261 aaaaactcag attttataaa ttctatacta ttagcagaag ttgaaaattt gactgaggaa
    46321 caaaaagaaa tttttactag aactggaact agtcatgtaa tatctatatc aggtcttcat
    46381 acaggaatat tatgtgtaat tatatccttt ctattaagag gtataaataa attgtataaa
    46441 ttattaatac tgtcgatttt tataacttta tattgtataa tggtaggtgc gtctccatcc
    46501 ataattaggt ctatagcatt tgttatgatt ttttatttat caatatttat tgatagaaag
    46561 aaagatggaa tatcagcatt gtctctaatt gggataattt taattatgaa taacccctat
    46621 gttatatata atgtaagttt tcaattgtct tttttagcta cactttctat attaatattc
    46681 tataataaaa taaatagtat tataaaatta agtatggtat cattgacaat atcatcaaat
    46741 atattaactt tacctataat ttattataca tttaaaggta taccattgct ttcaattata
    46801 ggaaatttaa ttatagtacc atttgtggga gtaataatgt atttaagtat tgcaagttta
    46861 atcgtattta aagtaagtgt agtaatagct aaaattatat ctttttttaa tagtacattg
    46921 atagaaagca tatttttttt actagaaaaa atcagcaatt taagttttgc atacattaat
    46981 atagaaagtc ctaaatttta tattgttgtt atttattaca taggagtgtt tttttacata
    47041 ttttacatag aaggaaaaga gataaaggag caagagaatg aattacaagg atattataag
    47101 gaatgtaaaa gagaaaaact ttgaaaaaat gtatcttttt tatggaagag agtattattt
    47161 aattgagaat gcaataaaag catttaaaga tagtctaaac gaaggaatgc ttgattttaa
    47221 tttagatata atagatggga aggaaatagt cttaaatcag cttataagct ctatagaaac
    47281 acttccattt atggatgata gaaagatagt aataataaaa gacttcgaac tattaaaagg
    47341 aaagaaaaag aattttacag atagtgatga gaaatactta atagagcatt tagataatat
    47401 tccagacact acaactatag tttttgttgt atatggagat gtagacaaaa gaaaatcatt
    47461 agttaagaaa ataggaaaca atggaattgt ttttgattgt gataagcttt ctgatatgga
    47521 tttatttaaa tggataaaaa aatcttttgc tttaaatgat gttataatag ataattctca
    47581 aatcatgtat tttattgaac aagaaggata cagagataag agcagtgaaa agacattatc
    47641 tgatttagaa aatgaaataa ataaaataag ttcctttgtt ggtaaaggga ataatgtaac
    47701 caatgacgta attgataaat tatctcaaaa aaaagttgag aatgatatat ttaaattaat
    47761 cgattatatt ggagaacaaa atgcttctaa tgcaatgaaa atattaaatg atatgataca
    47821 agaaggcgaa tctgtattag gtatattttc tatgatagca agacaattta aaataattat
    47881 gcaagtaaga cagttacaac ttgatggata ttcaacaaaa cttatagctg ataaattaaa
    47941 aatgcatcaa tttgttgtag gaaaagcatt aaagcaaact aaaaattttt ctgatgatat
    48001 aattgtggaa atattaaact atatattaga aagtgattat aaaataaaaa caggtcttat
    48061 aagagatact ttagcagttg aaatgttggt tagtagatac tgtaaaagag aagcaattta
    48121 aaaaatctat atattaataa aaaataaata gcaagatatg ttatagttca gagtagatgt
    48181 aagctaaatt aagtttgata tacaatcaac tataatatgt ctaactattt attttttatt
    48241 gtataaattt acaaaataaa aaacccctac ataggggtta ttatatataa gaaatataaa
    48301 ctggtaatta agcgttcata ccgtttaatt tttgagctaa tttagctact tttctagctg
    48361 cagcattctt atgtatagtt cctttagaag ctacttggta tattcttttt tgagcgtatt
    48421 ggaactttgc aacagcatcc tctctgtttc cagctgcaac agcatcttcg aatcttctta
    48481 ttgcagtttt tatttgagat tttctaactc tatttaaagc agttttcttt tcgataacac
    48541 ttattctttt cttagctgat tttatatttg ccaccgtgtt cacctccctg tattttttta
    48601 tcatattcat cgtcaacata ttagatttta acagaaataa atctaaaaat caaggattat
    48661 tatttatata ttttaataat gttttttatt ttgatttttt agactgtaaa ttgtgtataa
    48721 ataatttgca atatcttgtt tttaaattgg tatattttgt gttttaaata ttttgacata
    48781 tataaattaa tattt
//
LOCUS       DAMKPG010000019        46509 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMKPG010000019 DAMKPG010000000
VERSION     DAMKPG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.021"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            114..497
                     /locus_tag="PC621_001180"
     CDS             114..497
                     /locus_tag="PC621_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108350.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            1003..1386
                     /locus_tag="PC621_001181"
     CDS             1003..1386
                     /locus_tag="PC621_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF4108351.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1388..1738
                     /locus_tag="PC621_001182"
     CDS             1388..1738
                     /locus_tag="PC621_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108352.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1832..2290)
                     /locus_tag="PC621_001183"
     CDS             complement(1832..2290)
                     /locus_tag="PC621_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108353.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2335..3123)
                     /locus_tag="PC621_001184"
     CDS             complement(2335..3123)
                     /locus_tag="PC621_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4108354.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3262..3699)
                     /locus_tag="PC621_001185"
     CDS             complement(3262..3699)
                     /locus_tag="PC621_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF4108355.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4521..5030
                     /locus_tag="PC621_001186"
     CDS             4521..5030
                     /locus_tag="PC621_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108356.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(5941..6741)
                     /locus_tag="PC621_001187"
     CDS             complement(5941..6741)
                     /locus_tag="PC621_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108357.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(6936..8534)
                     /locus_tag="PC621_001188"
     CDS             complement(6936..8534)
                     /locus_tag="PC621_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4108358.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            8862..9038
                     /locus_tag="PC621_001189"
     CDS             8862..9038
                     /locus_tag="PC621_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108359.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            9212..10342
                     /locus_tag="PC621_001190"
     CDS             9212..10342
                     /locus_tag="PC621_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF4108360.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            10583..10933
                     /locus_tag="PC621_001191"
     CDS             10583..10933
                     /locus_tag="PC621_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF4108361.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            10941..11390
                     /locus_tag="PC621_001192"
     CDS             10941..11390
                     /locus_tag="PC621_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF4108362.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            11492..12067
                     /locus_tag="PC621_001193"
     CDS             11492..12067
                     /locus_tag="PC621_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF4108363.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12060..13469
                     /locus_tag="PC621_001194"
     CDS             12060..13469
                     /locus_tag="PC621_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF4108364.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            13653..15086
                     /gene="eutA"
                     /locus_tag="PC621_001195"
     CDS             13653..15086
                     /gene="eutA"
                     /locus_tag="PC621_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF4108365.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15104..16468
                     /locus_tag="PC621_001196"
     CDS             15104..16468
                     /locus_tag="PC621_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF4108366.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            16481..17362
                     /gene="eutC"
                     /locus_tag="PC621_001197"
     CDS             16481..17362
                     /gene="eutC"
                     /locus_tag="PC621_001197"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF4108367.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17382..18035
                     /gene="eutL"
                     /locus_tag="PC621_001198"
     CDS             17382..18035
                     /gene="eutL"
                     /locus_tag="PC621_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF4108368.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18046..18747
                     /locus_tag="PC621_001199"
     CDS             18046..18747
                     /locus_tag="PC621_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF4108369.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            18750..20219
                     /locus_tag="PC621_001200"
     CDS             18750..20219
                     /locus_tag="PC621_001200"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF4108370.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20315..20602
                     /gene="eutM"
                     /locus_tag="PC621_001201"
     CDS             20315..20602
                     /gene="eutM"
                     /locus_tag="PC621_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDF4108371.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            20729..21490
                     /locus_tag="PC621_001202"
     CDS             20729..21490
                     /locus_tag="PC621_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF4108372.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21503..22132
                     /locus_tag="PC621_001203"
     CDS             21503..22132
                     /locus_tag="PC621_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF4108373.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22174..22899
                     /locus_tag="PC621_001204"
     CDS             22174..22899
                     /locus_tag="PC621_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF4108374.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            22912..23184
                     /locus_tag="PC621_001205"
     CDS             22912..23184
                     /locus_tag="PC621_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF4108375.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23177..23728
                     /locus_tag="PC621_001206"
     CDS             23177..23728
                     /locus_tag="PC621_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF4108376.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            23740..24831
                     /gene="eutH"
                     /locus_tag="PC621_001207"
     CDS             23740..24831
                     /gene="eutH"
                     /locus_tag="PC621_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF4108377.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            24833..25306
                     /locus_tag="PC621_001208"
     CDS             24833..25306
                     /locus_tag="PC621_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF4108378.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25426..25527)
                     /locus_tag="PC621_001209"
                     /pseudo
     CDS             complement(25426..25527)
                     /locus_tag="PC621_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            25822..26343
                     /locus_tag="PC621_001210"
     CDS             25822..26343
                     /locus_tag="PC621_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4108379.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            26722..27897
                     /locus_tag="PC621_001211"
     CDS             26722..27897
                     /locus_tag="PC621_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108380.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            27932..29470
                     /locus_tag="PC621_001212"
     CDS             27932..29470
                     /locus_tag="PC621_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF4108381.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVLTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29502..30704
                     /locus_tag="PC621_001213"
     CDS             29502..30704
                     /locus_tag="PC621_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4108382.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(30883..31482)
                     /locus_tag="PC621_001214"
     CDS             complement(30883..31482)
                     /locus_tag="PC621_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4108383.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            31651..33009
                     /locus_tag="PC621_001215"
     CDS             31651..33009
                     /locus_tag="PC621_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108384.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            33002..33634
                     /locus_tag="PC621_001216"
     CDS             33002..33634
                     /locus_tag="PC621_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF4108385.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            33963..35120
                     /locus_tag="PC621_001217"
     CDS             33963..35120
                     /locus_tag="PC621_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4108386.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     FNSEEIVYFTPKGKSYHSIKNCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(35268..35903)
                     /gene="lexA"
                     /locus_tag="PC621_001218"
     CDS             complement(35268..35903)
                     /gene="lexA"
                     /locus_tag="PC621_001218"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF4108387.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(36007..37134)
                     /locus_tag="PC621_001219"
     CDS             complement(36007..37134)
                     /locus_tag="PC621_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF4108388.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(37456..38301)
                     /locus_tag="PC621_001220"
     CDS             complement(37456..38301)
                     /locus_tag="PC621_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108389.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(38331..38702)
                     /locus_tag="PC621_001221"
     CDS             complement(38331..38702)
                     /locus_tag="PC621_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF4108390.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            complement(38855..39115)
                     /locus_tag="PC621_001222"
     CDS             complement(38855..39115)
                     /locus_tag="PC621_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF4108391.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(39304..40248)
                     /locus_tag="PC621_001223"
     CDS             complement(39304..40248)
                     /locus_tag="PC621_001223"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF4108392.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(40269..41123)
                     /locus_tag="PC621_001224"
     CDS             complement(40269..41123)
                     /locus_tag="PC621_001224"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF4108393.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            complement(41138..42499)
                     /gene="accC"
                     /locus_tag="PC621_001225"
     CDS             complement(41138..42499)
                     /gene="accC"
                     /locus_tag="PC621_001225"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF4108394.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(42515..42967)
                     /gene="accB"
                     /locus_tag="PC621_001226"
     CDS             complement(42515..42967)
                     /gene="accB"
                     /locus_tag="PC621_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF4108395.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(43237..43683)
                     /locus_tag="PC621_001227"
     CDS             complement(43237..43683)
                     /locus_tag="PC621_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108396.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            43958..44236
                     /locus_tag="PC621_001228"
     CDS             43958..44236
                     /locus_tag="PC621_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108397.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(44365..45111)
                     /locus_tag="PC621_001229"
     CDS             complement(44365..45111)
                     /locus_tag="PC621_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108398.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(45240..45884)
                     /gene="trmB"
                     /locus_tag="PC621_001230"
     CDS             complement(45240..45884)
                     /gene="trmB"
                     /locus_tag="PC621_001230"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF4108399.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(46022..46342)
                     /locus_tag="PC621_001231"
     CDS             complement(46022..46342)
                     /locus_tag="PC621_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF4108400.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        17613 a         6164 c         7088 g        15644 t
ORIGIN      
        1 atattatact atcttttctt tttggaataa actatacttt aaatatagat atttaattta
       61 aataaatttt acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta
      121 cagacaaagt tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata
      181 ctggtaaaat ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc
      241 aactgagtaa ggatattttt aatgatataa tagacactat aaaagtggta ttaaagtcac
      301 ctgaatatat aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa
      361 ttgataatta tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat
      421 cttcaatgta tgctataaaa gaagcaaaat tagaaagtag agtaaacaat ggaagagtaa
      481 aacaatatga tggttaacat aatattatta attttcccca aaatattgaa aaatattatt
      541 atataagtta taatattaat atacacgtag atatttatta gttagatttg aggttggaaa
      601 aggcacccaa tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca
      661 aatctaatta aattaaaaag agaggattaa ttcctctctt tttgtttttt tgagatacta
      721 atttaatcta tatagaaaga ttgaaggtca tttatactaa aagttttcag ttatatacat
      781 taaactaatt aaataagata tttataatct atatatgata catattatat attaataaaa
      841 gaaaaatata tagaaaatcc ttataaaaat ttaaattgta atgaatagta agtaattaaa
      901 aagaattttt attgattttt aaaaatttag ttagaaacta tatcattatg gtaaagaatg
      961 tattataatg tttagaaata ttataataag gagcgattaa acatgaatct atcgaagaaa
     1021 atagaagata aaagaaaagc taaacagaga gctgaaaaag ttaagaaagc taaaattgct
     1081 acagctggtg tggtattagg agctgttact ggtgcagtga gtggagtact tttggctcct
     1141 aaatctggaa aagaaacaag agaggatata aaagatgctt cacaacaaat tgctgaaaag
     1201 ataaatatga aaactgtaga tgtaaaaggc aaagtatctg agaagctaga ggataaaaaa
     1261 ggaaacttta tagaatcaaa gaaaaaaata aaaaaatata tagataacaa aaagagtgtt
     1321 gaaaattcta gtgaggaaga taaaataata gatatagttg aaccagttag tgttactgag
     1381 gaataatatg gacaatacaa taacgattaa cataagtata atatatgtat tattagctat
     1441 acttgcttgt attgctttag tttatttaat tattgtactt gttaaactac ataagatttt
     1501 aaataatgtt ggaactatgt taaatgaaaa tactcaaaat ataaatacaa ctttaaataa
     1561 tcttcctaat gttgtagaaa cttttggaga agtaggagaa aatatgaaag atgtaacaga
     1621 agtagttact gaagttacag cagattttct tgttactaaa gacagtgtca aatcaaatat
     1681 ggatgttata gtcgatatat tgactataat taaaaatatt tttctagata aaaagtaata
     1741 aatttaatat aaaataagat ataatttaaa cgaccttaaa ataacattta taaggtcgtt
     1801 ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc ctctttatca
     1861 tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca tctttacatt
     1921 caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa aagatataag
     1981 aattatatag ccattcttca aatttttctt caagtatatc ccaatacgaa ctccaaaata
     2041 atccacaacc tgatatataa gatgctctac tttgatttat ataaacctca tttagaagct
     2101 tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt aattcattta
     2161 aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta taattttcat
     2221 caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct acaacttttg
     2281 ttcttatcat ttttttatcc taacttattt attttttata aaataatcat gattttataa
     2341 tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac catttattac
     2401 tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct ttttacctaa
     2461 accttgctta taactagacg cctcaattat taaagaatct ttcaattgcc aatgtaaaat
     2521 ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat acactttatt
     2581 ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg ctatcaattt
     2641 agcaacttta tctttattat taacataata atctgtatct gctttactat ctacaaaaca
     2701 tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac ctctaacatc
     2761 agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc tttctgcata
     2821 ggttttgcca ttattagtct tatatattgt ttctgtccct actggagttg aagttgtagc
     2881 atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg ctatagcaca
     2941 ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag tagctccacc
     3001 tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc tttcgtttat
     3061 atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta ttactacttt
     3121 caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc ataacctaga
     3181 attttgtttc tttttatatc ttatactttt attataaccc attttgtaaa aatatagaat
     3241 attaagtaaa taatgttaaa attataattt tgtatataag acattacttg attgcaataa
     3301 ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt taattccagc
     3361 ttgaattata gccttagtac aatttaagca aggaaagtga gttacataaa tagagcaatc
     3421 tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag cgtgaatggt
     3481 tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct tatctccaga
     3541 aatactacca ttatatccag tagacactat tctgttttct gaattgacaa ttatagctcc
     3601 tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc tcataaaata
     3661 ttcttgccat gagcatctat tttcaagttt tttattcaaa tcatatacct ccaaaattta
     3721 tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt atacgataaa
     3781 taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa agttacaagg
     3841 aatctaaaat tatatatatc tttcttcgta atatgaatgt tacaatataa aaaactgtct
     3901 caataaaata gagacagttt tacagtcaaa gtttataata caagttcata tttttcttta
     3961 aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc tttagctatt
     4021 ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt taatctccct
     4081 tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg ctgcatatac
     4141 aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct ttaaccgaca
     4201 tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt tttatttttt
     4261 tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata aatatttcta
     4321 cagttttaat tactggacat atctattttt attataaaaa gcgcttaaaa atcgagaata
     4381 tccgcgtaaa agctgtatat aagcaaaacg attaaaggaa aaagaagaaa tttacttgaa
     4441 tgttaaaatg cgttgcttga agcaacgggc agttttgcct ataatatcgg taacaactga
     4501 aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg aagtcaaaag
     4561 gactttcaca acaagaactt gctatcaaac tgaatgtagt gcgacaaaca atatctaaat
     4621 gggaacaagg actgtcggtt cccgattctg atatgttgat ttccatatca gaggtgcttg
     4681 aaacgcctgt aagcacttta cttggagaaa ctattgttga gtcggaagtt gatagcttaa
     4741 aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga aaaaacatga
     4801 gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatagta atagtttttg
     4861 tggtcttagg gggactaaat agtccttact tggattggaa ttataccgac cctgaaaccg
     4921 ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta gtaccgattg
     4981 tccttatagg agcaattatt ggaatctttt tgacacgtaa gaaaatatga agtaactctg
     5041 ctttttataa tatagcaagc gttccttaaa agtgtcattc tacatggcaa tcgaggacaa
     5101 ctatgtaagg aagaaccttt ttgatttcca actaagcgaa gttattgagg acgccagtag
     5161 gcccaaaacc gcattaagta aagtacagga agcaagactg ctggacttat actctcatgc
     5221 gtcagatgca gaaaatgcga ggtctgtagc atgatttaca acgatttgta caacgactac
     5281 gagtagaaat atatggagat aagtattatc tacgaaatca gaaaagaaat gacttgataa
     5341 tttactcatg ttataattat tgatgtgaaa ttaataaatc gaaattcctt ttttagattt
     5401 aacaatactg agtaaggaaa aagtgaatga cttttgcaag gaggtgttaa tatcaggcaa
     5461 ataaagtctt ttgtttttcc agttatatcc ttaatattct tagatcaaat tagcaaagtt
     5521 cttataggat tattcttaat ggactttgaa attgatataa ttgggaaatt tttaagattc
     5581 aatcctgttc aaaatacaaa attaagtctc tgctatctaa caaacggagc acaaaaatga
     5641 aaattaaata ttaaatataa agcacagtta attttgatta aaaagttgac tgtcttttct
     5701 tttttataaa tttaagcatg ggtaataaag aaatgactgc atttaagcat tatgtatata
     5761 attggaggca ataaaaagcc agcaaagccc ataaaataga gctgtgctag caacatgatg
     5821 agttataaga agataatcaa aagcttacgc tttattttaa tttaaaaaaa tgattactgt
     5881 aaagaaatca ttttttatta taatcactga tacatcaaat attctaaata aaatttttat
     5941 ttatctggaa ttgtatattt tttctataat attaactaca gcgtacattg cccaagcaca
     6001 aatagccaat acaaatactc ccatcattac taaatctaat ttaaatactt gagaaccata
     6061 tactatcaag tagcctattc catatcttga aactaaaaat tctccaacta ttacacctac
     6121 ccatgccata ccaatattaa ttttagttaa atttattaaa tttcctgtgt ttgcaggtaa
     6181 tatgagttta aataatattt gagatttagt agctccaaag ctttttaaca tcttaatttt
     6241 ttcttcatct atattcataa aataattata agctgaaagt atagtaacaa caacagaaat
     6301 cgttacagct gttacgacga ttccttctat tccagcacct acccaaacta taattatagg
     6361 agctaaagca gtcttaggaa gagcatttaa aactaccaaa aatgggtcta atatttttga
     6421 taatttttct gaccaccaaa gagcaattgc taccaaaata cctaatacag tacctataac
     6481 aagaccaaga acagtttcat atacagatat tccaatatgt ttaaataatt gaccactaaa
     6541 tgcatattga ataaataaat tgtaaatatc acttggtttg ctaaatagaa atgtatttat
     6601 aacatttaag tcagctaaaa gttcccagat tacaataaaa ccaattaaaa ttaatagctg
     6661 gtagaataat acatttcttt tttctctttt tacaccttta acatagttta tatatccttc
     6721 agatctttta ttaagcctca tgtgtatcta gctccttcca taatatgtcg aaatactttt
     6781 gaaaatcagg aactttacgt gttttgagag gcgtttttgt tccatccatg ttaagttcaa
     6841 tatcgtaaat attttttatg gtagctggtc ttttagatag tattgcaacc ttatctgata
     6901 tgcttattgc ttctgctata gctaaaaaag tgcaactaat tgcttaaaga aacagaatta
     6961 tccacttcag cacccatata aacaatatta agatttccta aactgctatc ccaaatgata
     7021 ctattaataa tagtagataa taacaatctc ttatcatcta aactggcatt atcaacttca
     7081 tcattaaatc tcttcaaatt actaacaata atatcaatat taatactgtc actactaata
     7141 ttgatattac tagattccaa aatattaatc ttatccttta aaatactaat ctcattattt
     7201 aggctctcaa tctgggaaac tatataatca gaagcagtac taccagtatt ttttgcaagc
     7261 tctataacta aatttttaat aagtctttcc ttatctaaaa tttgattttt aagtaaatta
     7321 gtctcagatt taaaaatatt aaaaacatta tcagtcttaa tgttattact attgaatgca
     7381 cttataatag actcttcttt acaattctta attttatcta taactaaact ttcaactaat
     7441 ggaccattaa gatttttaca gttgcaacta tcagacccaa gtgttttttt agtaccacaa
     7501 atataataat actttaaatc cttgtcagtt tttttgcctt tataggtaat tctcatatta
     7561 gaaccacatt taccacattt tataagacca gataaaagag cagtttttcc agttgatatc
     7621 cttggagttt tatcacgatt tttatctaat tgattttgta cttcaagcca tatttcaggt
     7681 tctatgactc catggtgttt tgcaattgct gctattgagt tagatgtatt tttagcatag
     7741 gtaaggattc cacattttct atctggagta cctacaacat ccataccact atttttaagg
     7801 taatctaaaa tttcatcatt agcttttaca tatgctggat ttctaagtat tctagataaa
     7861 gaactagggt caaatttacc accacgagga ccccttattc cattattata gacatattta
     7921 tataacttag ataaggacct aagttctaaa tatttattgt atattagctt tacaatctcc
     7981 atttgttcat catttacttg aagcttatac atacttcttt cttttagatt ttcatctaag
     8041 aatgtgattt tttcactgtt gaatccatga ggtgtattgc ctccaagcca tctaccacta
     8101 cgggctaatt catacatgtt gtcttttata cgttctgcta ttgtttctct ttctaattga
     8161 gcaaatacac ttgatataaa catcatagct cttcccattg gagttgaagt atcaaattgt
     8221 tctttgatag atataaatga tatgtttagt tcatttaatt tttctattaa agtagagaag
     8281 tcagatacat ttctcgatat cctatctaat ctataacaaa ctaggtaact aaatttctta
     8341 tctttagcat cctgcatcat tttcttaaat tgtggtctat ctatattgct accactaaat
     8401 ccctcatctt catatatcaa aaaatctttt acaccaatag actttgcata atcaatacat
     8461 aattgtattt gattttctat agaatcacct tttccagtaa atcttgattt tcttgaataa
     8521 attgcagctt tcaaaattaa aacctcctat aaattaggat atatatttga aattatttat
     8581 atacaacctt ttaagtaaac ttattagaat aaatttatta attaggtaaa tttatataat
     8641 tctacttttt ataatatatg actatttatt ttatttcttt actattcaag ttctaaatca
     8701 ataataaatt ttgatattta attgtatcag gacaaatctc taatttcata tattattaat
     8761 ataaaatcat aggttagtgt gaaaaagttg aaatttaaat attttaaaag ataatttttt
     8821 ggaactaata ggtataacaa aaatttatag ggggattgca tatggaaatc aatgttatag
     8881 aaattttccc taaagataaa gctaaactta ataaaataga aatggataaa gctagttggt
     8941 ttgtaaatat aataagtaaa aaatatccta aagaagcttt aaatgaagca tttagtattt
     9001 tagaaaaaga attaaatata agtaaagcta atacataagc tttgattatt gattttatat
     9061 actattggtt ttatacgcta tgacttgaca ttatattaat caaatagtat atttttaaat
     9121 atagcacaat gaagtgttaa aagagcaatg gagctccata gataaagtct atggagcttt
     9181 tttgcgtatt taacttaagg aggatagagc tttgaataac tttgagatta aaactagaat
     9241 aaaatttgga gaaggttcac taaaagtttt gcaagaatta aaaaacaaaa atgtattaat
     9301 aataacagac ccttttatgg tgaaatcaaa aacaatagat aaaataacat ctaatttaat
     9361 aaactcaaca tatcaagttt ttgaagaaat agtaccagac ccaccaatag aagtagtagt
     9421 agcaggaata gaagcattta aatctataaa tccagacgta ataatagcat taggtggtgg
     9481 ttctgcaata gatgcagcaa aatctataat ggacttttct aaaaaaatat taaaaattaa
     9541 cgaagtagag tttatagcaa taccaacaac aagtggaaca ggttcagaag taacatcatt
     9601 tgctgtaata acagatagtc aaaaaaaatg taagtatcca ttagtgagtg atgatttact
     9661 tccagatatg gcaatactag acccagagct tgtaaaaact gtaccaaatt ttataacagc
     9721 agatacaggg atggatgtat tgacacatgc tatagaagct tacgtatcta caaattctaa
     9781 tgacttttca gatgcactag ctgaaaaatc tataaagtta gtatttaaat acttaataaa
     9841 agcatataaa gatggaaatg acatagaagc tcgagaaaaa atgcacaatg catcttgttt
     9901 agcaggtatg gcatttaacc aagcatcact tggcataaat cattctattg cacatgtatt
     9961 aggtggaaaa ttccatatac cacatggaag aacaaattct atattacttc ctcatgtaat
    10021 agaatataat tcatcaatag taggatatac taacacaact tactctgata cagctaaaaa
    10081 atatgcacaa atagcagaat tagtaggact tgggagtgct aatataagaa tatctgttag
    10141 aaatttagta aatgaaataa agaaactaca aaaagaaatg aatatgccaa ctaaattgac
    10201 acaatgcaaa ttaagtttag aagatataac taatgaagaa agagaaatag caaaacttgc
    10261 tataaaagat acttgtacat taactaatcc taaggtacca actgaagatg atataataaa
    10321 catattaaat aacattaaat aactacattt tattacatgt aaatataata ttatacaaat
    10381 agggaaaatg tttttaaaaa tataaattat atattgacaa atataaaaca taaaactata
    10441 atgaaagtat cacaaaggcg tgatacttta gtagaaagca atggagcttc ttaggaatat
    10501 taatattatt cttatggagc tccttttatt ttaagcaaat agctttttaa taaactatat
    10561 tcaaagaaag cagggaataa atttgactga agaatctaag caaagagtta ttcaagaata
    10621 tgttcctgga aaacaggtta cattagcaca tatcatagct aaccctaatg aagacatata
    10681 taagaagtta ggattagtac ttgataaaag agatgcaata ggaatactta caataacacc
    10741 aagtgaagcg tcaataatag cagcagatgt tgctacaaag gcttcaaatg tatctttagg
    10801 ttttatagat agatttagtg gttcagtagt aatatcaggt gatgtaagtt ctgtagaatc
    10861 agcgttaaat gatgttttag aagtcttagg aaatatgtta aacttctcat ctacaaaaat
    10921 cacaaggaca taataataca atgaagaata taatatttat gggaaagaca ggtagtggta
    10981 aaactacctt atgtcaaaaa ctagaccagt tagaattaaa gtataaaaaa actcaatcag
    11041 tggaacttta taataatgca atagatactc ctggtgagta tatggaaaat agaaacctat
    11101 ataatgcctt aataacaaca tcagcagatg ctaaaattat agcaatagtg tatgatgcca
    11161 cacaagaaga aaattatata gcaccaggtt ttgccagtat gttttgcaaa gacgtaatag
    11221 gaattataac taaaataaat aaagctactg aaaacgaagt caatatagct tatgaaagac
    11281 tatcactagc aggagcaagt caaatcttta aaatagacac agttgatgat attggacttg
    11341 aaaaattatt taaatatcta gaaaaatcta aaaatataga tgaagtttaa ggggaaatat
    11401 ttttttattc aaaaatatta ttaaagtatg ataaaataaa aaaatgtata aactatttta
    11461 agaagattta atctttaagc gaggtaggaa catggggaaa aaaatactat tggttgatga
    11521 tgaaccactg ttaaggatgg atacaaaaga tatattagaa agtcatggct atgaaataat
    11581 aggcgaagca agtgatggat ttgaagctgt tgaaatgtgt aaaaaacaca aacctgattt
    11641 agttataatg gatatagaga tgccaatact tgatggtata aaggcaggca aaataatatg
    11701 taaagaaaat ctagctggtg gagtactttt attaacttca tttgaagata aagaatacgt
    11761 agaaaaggct aaaagtatag gtgcttatgg atatttagta aaaacagcaa gtgaaaaatc
    11821 acttatccca accatagaaa tgtgtttaaa taaggtaaat gaatttgaaa aaatgaaaaa
    11881 agatttagaa aaagtaaatt ctaaattgaa tgaaagaaaa ttagtagaaa gagcaaaagg
    11941 tatacttata agagagttta agatttctga ggatgaagca tacactagaa taagaaaatt
    12001 aagtatggat aaaagaacta caatgatgga aattgccaaa atgattataa taggatacga
    12061 tgactaatac gataaaaaaa atatcaactg cacaaacata cttaaataat aacgaaattg
    12121 aaaaaattat agaagtatca aaatccttag atttaatggc ttctttttat gaggctgatg
    12181 tttttataga tgtactaagt aaatttgatg atgaagcaat tgttgtggca catggtagac
    12241 ctaaagaaaa gtcaatgtac tgtgaaaatg ttataggtaa aaaggcttta attgaaaatg
    12301 aaccaggagt tataaagtgc ttaaaaacag gagaaagagt aagagatatt aaagctttaa
    12361 cacaggaata taaactggtc aaacaaaata ttcagccaat aacactagat gacaaagttt
    12421 tagcagtact aatagttgaa aaagatataa gtagtgaagt taagaatgag tttaatgcta
    12481 gtgaagacga agaaaataaa aatttacatc agtttataaa acttatcgga aataatgact
    12541 ttttagttga taatttaaat tcagccatat taatatttga taaaaatgga aaattaaaaa
    12601 ttaaaaatcg tattgcaact gagatataca aaaatcttgg gtttggatat gatatacaag
    12661 atatgcatta caatgattta gatatagaaa ccaacacctt tgaaaatata ttaatggatg
    12721 aaaattatga tgagacaaat gaagtttcaa taggtaatat gttttttaaa ataaagacca
    12781 tatacatcaa agatgatgaa tttaaggttt gcaaaatagt acaagatata agtgatttaa
    12841 agagaaaaga agaagaatta gttttaaaaa cagtggctat aagagaagct catcatagag
    12901 taaaaaataa tcttcataca gtaatatcta taataaaaaa acaaagtaga ttatctcaaa
    12961 atgatgaagt aaagcagtgt ttagataaca taataaacag agtatttgca atactttcaa
    13021 ctcattattt actatctaaa gaagttgata ataatatatc tatacgagaa ggtatcaatt
    13081 tattagtgtc aaatattcaa ggtggctatt tatttagtaa ggatatagat atacgtataa
    13141 ctggtgaaga ttttaaaatt agtggagaaa aagcaacagc aatacttttg gttataaatg
    13201 aaatactaca aaattgttat gaccatgcat tttcggatag agaacatggt actatacaaa
    13261 tattaataaa taaagataac gactatagaa atattgctat tatagatgat ggaattggat
    13321 ttgatagtaa taatataaat aaaaatagct tagggacata tataatagat agttatataa
    13381 cacagatgtt aaaaggaaat atagagagaa aaagtaatga gaatggtaca gaagtattaa
    13441 ttactatacc tacacaaatt aatatttaaa taataactca tctacaaagt agtagtttta
    13501 attgagcaaa gaggcttaag gttattaata gattaagtta attgaatgta tttataaaaa
    13561 ttataagtat atattcaatt taacaggtat ttattataac tttaagcctc tttgcttgtt
    13621 taagacatac ttaaatttta ggaggtgaga aaatgagaga agagttatta agtgttggta
    13681 ttgacattgg aacaagtact actcagttaa ttttttctaa attaatagta gaaaatactg
    13741 aatcaaattt ttcggtacct agaataagta tagtagacaa agaaattatt tataaaagca
    13801 aagtttattt cacccctctt atttcaaata atgaaataaa tggtcaacaa ataaaagata
    13861 tagttgaaaa tgaatataaa aaagcaaata tatctaaaga ggatatacat acaggagcag
    13921 taataataac tggtgaaaca gctagaaaag aaaatgcaaa tgatgtactt catactttga
    13981 gtggatttgc tggggacttc gttgttgcaa cagcaggacc tgatttagaa agtataatat
    14041 caggaaaagg agctggagct catatttatt caaaagaaca ttcaactaca attgtaaata
    14101 tagacatagg tgggggaact agtaacatag cctcatttaa aaggggagaa gtaaccgata
    14161 ctgggtgttt ggatataggt ggaagacttg tgaagataga taaaataagt aaaaaaataa
    14221 cttatatatc acctaaaata gataaaatca taaaagataa taatttaaat cttcaagtag
    14281 gcacaatggc tactgtagaa aatacaaagc caatagtgga tataatggtt gacttattaa
    14341 tggaaagcat aggattaaag aaacagacac ctttgtttga ttttataata accaataaat
    14401 caataagtat agatgaaaat attaaaaatg tttcattctc tggtggagta gctgattata
    14461 tttactatga tggagaaata aaagattatt ttgaatatgg tgacttggga attttacttg
    14521 ggcaaacaat aaaaaattgt gaatacctta aaaaagtaaa tgtagttaaa tctatggaaa
    14581 caataagagc cacagtggtt ggggcaggtt ctcatactac tgaagtaagt ggaagtacaa
    14641 taacttacac tagcgaaaag tttccactta agaacttgcc agttttaaaa ttatcagttg
    14701 atgaagaaac aggtacttca aaacaaatca gtgaagctat aaaaaataaa cttaattggt
    14761 ttaaactaga aaatgatttg cagaaagttg ctatagccat gataggaaag aaaaatccaa
    14821 cctttttaga tatacaagag tatgctaaag ggtttgtaga tggaatgcaa caaatcatag
    14881 aaaatgattt accttttata gtaattgttg aaaatgatat ggcaaaagtg ttaggtcaag
    14941 caatatatgc attattaaat tatgaaaaag aaattgtttg tatagacagt gtcaaggttg
    15001 aaaatggaga ctatatcgac attggaaaac ctatagttga aggtaaagtt ttacctgtgg
    15061 taataaaaac attagttttt aattaataga aggaggtaag taaatgattt taaatacaaa
    15121 attatttgga cgtacttatc agtttaaagg cttatatgat gtaatggcta aagctaatga
    15181 agaaaaatca ggagataaat tagcaggtct tgcagcagaa tcagctgaag aaagaatagc
    15241 agcaaaagtt gttttatcac acataaaatt ggaggatata agaaataatc ctgcagtggc
    15301 atatgaagaa gatgaagtaa caagaataat acaagatgct gtaaatgaaa aagtttatga
    15361 agaaataaaa aattggacag tatctgaact tagagaatgg ctattggatt caaatactac
    15421 aagtcttgat ataagaagaa tatcaagagg attaacttct gaaatgatag cagcagttgc
    15481 aaaattaatg tcaaatatgg acttagttta tgcagctaaa aagataaagg ttactgctca
    15541 ctgtaataca actataggag aacaaggaac attatcagca agacttcaac ctaatcatcc
    15601 aacagacgac ccagatggaa tacttgcatc acttcttgaa ggtttaactt ttggtatagg
    15661 agatgcagta cttggattaa atccagttga tgactctgtt gagagcgtta ctaaagtatt
    15721 aaaaagattt gaagaagtaa aacaaaaata tcagatacca actcaaacat gtgtattggc
    15781 acacgttact acacaaatgg aagcaataag aaatggtgct cctgcagatt taatattcca
    15841 atctatagca ggttctgaaa aaggaaatga agcatttgga tttaatggcg acactataga
    15901 agaagctaga cagttagctt taaaacacgg tacagctaca ggacctaacg ttatgtattt
    15961 tgaaactgga caaggttcag aattatcttc agaagcacat catggaatag accaagttac
    16021 tatggaagct agatgctatg gatttgctaa aaagtatgaa ccattcttag taaatacagt
    16081 tgttgggttt ataggacctg agtacttata tgatgctaga gaagttgtaa gagcaggact
    16141 tgaagaccac tttatgggta aactacatgg cttaccaatg ggtgttgatg tttgttacac
    16201 taaccatatg aaggctgacc aaaatgatat ggaaaactta gccatactat taacaacagc
    16261 aggatgtacg tattttatgg gaatacctca tggagatgat gttatgctta actatcaaac
    16321 aacaggatac catgaaacag catcacttag agaaatgttt ggactaacag caataaaaga
    16381 atttgatgct tggatggaaa agatgggatt ctataaagat ggtaagttaa cagagttggc
    16441 gggagacgca tctgtattat taggatagga ggtatttaat atgaatgaga aggatttaaa
    16501 agcattggta gaacaattag ttggacaaat ggttggagaa ttagatacta atgttgtatc
    16561 tgagactgtt aaaaagacaa ctgaagttgt agtagacaac aatgctttta tagatgatat
    16621 aacagaagtt gatataagaa agcaattact agtaaaaaat cctaaggatg cagaagctta
    16681 tttagatatg aaagcaaaaa cacctgcaag attaggtata ggaagagctg gtactagata
    16741 taaaacagaa acagttttaa gatttagagc agaccatgct gctgcacaag atgctgtatt
    16801 ctcttatgta gatgaagaat ttataaaaga aaataatatg tttgcagtgg aaactttatg
    16861 taaagataaa gatgaatact taacaagacc agatttggga agaaaatttt ctcaagaaac
    16921 tataaataat ataaaatcta aatttggtac aaatcaaaag gttttaatat tagttggaga
    16981 tgggcttagc tcagctgcaa tagaggcaaa tttaaaagac tgtgtgccag ctataaaaca
    17041 gggtctaaaa atgtatggta tagattcaag tgaaatttta tttgttaaac attgtagagt
    17101 tggtgctatg gaccacttag gtgaagaatt aggttgtgaa gttatatgta tgttggttgg
    17161 agaaagacca ggattagtta ctgctgaatc aatgtcagca tatatagctt ataagccata
    17221 tataggaatg gcagaagcaa aaagaacagt tatatcaaat attcacaaag gtggaacaac
    17281 tgcagttgaa gcaggtgctc atatagctga acttataaaa actatgttag ataaaaaagc
    17341 ttcaggaata gaccttaaat aaagtcgagg gagtgtagaa tatgaaaaat gatgtaattc
    17401 gtcctaatat actaggaatt aaaataattt cgaatataag tccagaaatg gcacaaaaac
    17461 tagaattaaa atcacatcac aaaagtttag gtttaataac tgcagactgt gatgatgtta
    17521 cgtatacagc tcttgacgaa gcaactaaag cttcagaggt tgatgttgta tatgcaaaat
    17581 caatgtatgc aggtgctgca aatgcttcaa caaaacttgc tggtgaagta ttaggaataa
    17641 tagcaggccc aagcccagca gaagtaagaa gtggtctaaa tgcagtagtt gattttctag
    17701 agtatggagc aacttttata agtgcaaatg atgatgattc aatagcatac tatgctcact
    17761 gcgtatcaag aacaggtaca tacctttcag agatagcagg aataagagaa ggtgaagcat
    17821 tagcttattt agtagcacca ccacttgaag caatgtatgc actagatgca gcaatgaaag
    17881 ctgcagatgt taagatgtgt gaattatttg cacctccaac tgaaacaaac tttggtggag
    17941 cattacttac aggttctcaa tcggcttgta aagcagcatg tgatgcattt gcagaagcag
    18001 taaaatcagt tgcagacaat ccaacaggat tttaaggata gttagatgga tgctataggc
    18061 ttaatagaag taataggata tgttgctgca atagaagctt ctgatgcttg tttaaaatct
    18121 gcaaatgtta atattgtaag aattgataag gttggggcag gtattgttac tttaacaata
    18181 tgtggagatg tcggtgctgt aaaagctgct ttagaagcag gtgaaatagc agcttccaga
    18241 gttggtacat taagaacatc acatattata ccgagaatcc ataatgaagt aacaaatgct
    18301 ttattcaaaa ctaaagaacc taaagtctgt gaagtttcag aagataaaga aaaaaacttt
    18361 gagattacat tggaaataac tgataatgta tcgaaaaatt tatcagaaaa agaaaatgta
    18421 attttagatg tcaatgaaaa cagtggagat actgaaaatt tatatagtga tatagataaa
    18481 acaaacaaaa aaattgaaac agttgatgat agtttaacag aagctaatga ggaaaaaata
    18541 gaaaatacta atgataaaga agtattggaa caagttaaaa tagaaaattc cgattttgat
    18601 agagatgacc tatcaaagaa cagtgtaaaa aaacttaaag ctatggctaa gaaattagat
    18661 tcttcaataa cttataaaga tttaaatgtt cttaaaaaag aagaattaat aaacttagtt
    18721 aaaaagctta gcagaaggga tagatagtta tgacactttt agataaagat ttagtatcta
    18781 tacaagaagt cagaactctt gtaagaagat ctaaagtggc tcaagagaaa ctttctaaaa
    18841 tgagtcaaga gcaaatagat aaaatagtaa aagctgttgt agatgcagca tttaataatg
    18901 ctactaaatt agctaaaatg gcaaatgaag aaactggatt tggaaaatgg gaagataaag
    18961 taattaaaaa tacttttgca tctatgggag tttatgaaac tttaaagaac acaaaaacta
    19021 ttggaataat tggtgaagat aaagaagcca atacgataga tatagctaca ccagttggag
    19081 ttgtggcagg tcttatacct tcaacaaacc caacatcaac agtaatatac aaaactataa
    19141 tatctctaaa agcaggaaat actatagttt tctctccaca tccaaatgca aaaaactgca
    19201 tacttgaaac agttaatata ataagtgagg ctgctataaa ggctggagct ccagaggatt
    19261 caatattatg tataactaat ccaagtatag ttggaaccaa tgaattgatg aaacataaag
    19321 atacaaactt aatactagca actggaggag aagcaatggt tagagctgct tactcttcag
    19381 gaacaccagc tattggagtt ggaccaggaa atggaccagc atttatagat aaatcagcaa
    19441 atgttaaatt agcaatcaaa agaataatag attctaaaac ttttgataat ggtgttatat
    19501 gtgcatcaga gcaatctata gtaatagaaa aatctatgga agaaactgtt ttaaaagagc
    19561 ttaaaaatca aggtgcatat gttttaaatg aagaggaaag tgaaagatta gcaaaattca
    19621 tattaagagc taatggtact atgaatccac aaatagttgg taaaagtgtt gaggtaatag
    19681 caaatttagc agacttaaca aatataccta aaaatacaaa agttctagtt gcaagagaaa
    19741 ataaagttgg tcacaatgta gcctattcaa gagaaaaact tacaccaata ctagcgtttt
    19801 atgttgaaga aaatgtagaa gctattatga acagatgtag agaaatattg ttaaatgaag
    19861 gtgcaggtca tacattctca atgcatgtaa atgatgaaga attggtaaaa agatttgccc
    19921 ttaatatgcc agtatcaaga atacttgtga atactccttc agcacttgga ggaataggtg
    19981 catcaacaaa ccttgtacca gcactaacac ttggttgtgg tgcaataggt ggaagttcaa
    20041 cttcaaacaa tataggacca cttgatttaa taaatgttaa aagagtagct tatggaatca
    20101 aagaaataga agaacttgga agagataaat ctcaagttga aaaatcaatg tttgactcaa
    20161 tggataaaga agaattaata aatcttcttg ttcaaaaact agtcaaagaa ctagcataac
    20221 taaaataaac aatgaaactt atggctatcg ctataaattt ataaaaatat aaactataaa
    20281 aaactataaa attaaatttt tggaggataa gattatggca agtgcaaacg cattaggaat
    20341 gatagaaact aaaggattag taggagcaat agaagcagca gatgcaatgg ttaaagctgc
    20401 aaatgtacaa ttagtaggaa aagagcaagt aggtggagga ttagttactg taatggtaag
    20461 aggagatgtt ggagctgtta aagctgctac agatgcagga gctgcagctg ccgagagagt
    20521 tggagaacta atatcagttc atgttatacc aagaccacac tttgaagtag atgctatatt
    20581 accaaaggta tcagctgagt aatataagaa taattttaaa atttaaattt tgattgaaaa
    20641 agaggctatc tcaaaaatga gtcttactaa taaactcaat gtattttgag gtagtctttt
    20701 ttatacatct aataggagat gtgataaaat gggtgtttta acagaaaacg aagtaaaaag
    20761 aaaaattgga aaaaacaaaa ataatataac tgaattttat atagaaaaag gtcaaataat
    20821 aactccttct gcaaaatctt atttagctga gagaagtatt gagcttaaat atgttgataa
    20881 actttcagat gtaaacaatg aacctaaaga agttgtaaaa gaagtagtta aagagaaaaa
    20941 atataaatat actactgtat ttggcgctaa attggatgaa aaaccagaac atatgactca
    21001 attaaatgga aatcttttag tttttaaaga ccataaaaga ataatattga gaggaaaaat
    21061 agattctctt gaatcagaaa tactaaaggt acagatactt tgtcagaaac tagatatgcc
    21121 aaagcttgtt aaggaattgc aagaagtatt agactttgta agaaatataa tgagatgtga
    21181 agttttagat gaaaagctag aagactttaa acttttagga ttaactcctc aagaacttag
    21241 agaacaatct cactatccta aaaaatactt tggtatagga catgaattcc caagttatga
    21301 aatgggagaa gtagtagtag ctataaatag tataagaagt aatacacgag aggttgaact
    21361 tgcagcctat gaagcattta agggtgagta tggaaatgtt cacagagaag atttaataaa
    21421 ggcacttaat agattatcgt cagtattttg gataatgata tataaggtta gaactaataa
    21481 gtataagtag ttggaggaca taatggagaa tctattaaat gaaatagtgg aagaagtaat
    21541 aactagagtt aaaaaagaag catttataga agttgaagca tcaggaagac acgttcactt
    21601 aagtagagaa gatgtcgata aattatttgg agaaggatat actcttacaa aactaaaaga
    21661 cctatctcaa ccaggtcaat atgcatgtaa ggaaagagtt acaataactg gtcctaaagg
    21721 aagtataaaa aatgtagttg tacttggacc atgtagaaat gaaactcagg tagaaatatc
    21781 tttaacagat ggttcaactc ttggactaaa agctcctata aaacaaagtg gagacttaga
    21841 aggtacttta agtattaaaa tatcaacaca atatggtgaa gttgaacttg ataaggggtt
    21901 aatggttgct aaaagacata tacacatgac tcctaaagat gctgaaaaat tcaatgtatg
    21961 tgataaagag atagttgaag ccaaggtgat gggtcaaaga cctttaacat ttgatgatgt
    22021 agtaataaga gtgagtgaca gttttaaaac ttatatgcat atagactatg atgaagccaa
    22081 tgcatgtggt tattcaaaag gaactgttgc taaaataata aagaaagcat aaatttaacc
    22141 tactatataa attacctcta gggaggattt aatatgaatt atgataacct tgtagattta
    22201 ataacggaag aaatctataa aaaaataaat agtaatgaaa taaaaatatc taataaacct
    22261 aaagctgtga ttgtatttga acaagataac aataaattta acttattaaa agatgaattt
    22321 gaaatactag aatttgataa aagtataagg gaatgtgaaa tagtgatagt ttcaagactt
    22381 tgtatgaggg ggctatcgaa tatagcactt ggaaattcaa caagtgatga agaaagattt
    22441 atactaaaaa tgattatgaa aggcaaaaaa gtatatgtcc tagatgaagg aattgaatac
    22501 aaaaaatata aggatactgc accaaaagca ttatataaga aatttatgtc atttgaagat
    22561 gaaatatgta aatttggtgt agaaattata aaagacttaa atgttataac taaaaataag
    22621 ttaaatataa agaaagaact agaaagtact agaggttcta aaaaagaaga tacaaaagtt
    22681 ttagataaag atgttttaaa tttaaccagt gtagaaagtg taaaaataga taatgaattt
    22741 agtttggatt taaggaataa aaaacttata tcggaagctg acttaagaaa accaactata
    22801 aatggagtta aaaacatatt agttaataaa aaaagtataa taacaccatt agctgtagat
    22861 tttatgagaa tacaccatct caaactaaag aaactgtaag gaggtaattt tatggatata
    22921 ggaaaagttg taggtaatgt ttgggctaca agaaaagatg aaaaattatg tggccaaaaa
    22981 cttttagttg taaaaatttt aaaaactaaa gacacataca aggaaggctt catggttgca
    23041 gccgataatg caggagcagg aaatggagac ttagttctta tatccaaagg ttcctcagct
    23101 agagagtcga ttgaaaaatc tcatgttcca gtagatgcaa ctatagttgg agtagtcgat
    23161 tcgtttgagg tgtttgatga ataaaagtat aggagctata gagtttaaaa gcattgctaa
    23221 aggtatagaa gtatccaatg aaatgataaa gaaatcttca gttgatgtat tgtacttaaa
    23281 aagtatttgc ccaggtaaat ttctaataat agtaggtgga gaaacttctt atataaatga
    23341 atgtgtagat tatggaataa aacttggaga aggatatatt gtagataact tcgtaataaa
    23401 tgcaatttca caagaaatat tagatggatt taaaaataag tatcaaaagc tagatagtat
    23461 agtctctata ggcgttgttg aaagtagtaa agtatgcgct ggaataaaaa tgcttgataa
    23521 gactttaaaa tctggcgact tagtattagt aaaacttcaa ttatcttttg ctataggtgg
    23581 caaattagta tatgtagtag caggagattt aagtagttta gaatatgcat taaaagaaag
    23641 tgaaaatgta gtaagagaaa aagaagtaat atataagact gtaataccat cagtagacag
    23701 tcaaataata aaaagtctaa taaaataatg gggggaatta tgagtataaa cgaaattata
    23761 atctatgtaa tggtagtctt catggtactt ggagctatag ataaatgtat aggtaacaaa
    23821 tttggtcttg gagaacaatt tgaagaaggt ataatggcaa tggggtcact tgcagttgca
    23881 atggttggtg ttatatgttt agcaccagtt cttgctgatg tattaagacc aattgtagta
    23941 ccagtattta atatacttgg agctgaccca gctatgtttg ctggaagttt attagctaat
    24001 gatatgggtg gtgcgccact tgcacttgaa cttgcgaaag accctaattc tgggttattt
    24061 ggtggattaa tagtaggggc tatgatggga cctacaatag tatttataat accagttgcg
    24121 cttggaataa ttgaaaaaga agaccaaaaa ttcttagcaa caggaatact tgcaggtata
    24181 ataacaatac ctattggagc attcgttggt ggattagttg caggattcga ggtaatgatg
    24241 gttcttagaa acttagtgcc aataataata tttgcagtag ttatagcact tggattatta
    24301 aaattcgaaa atgctatgat aaaaggattc acatactttg gaaaaggtgt agtaatagtt
    24361 ataactttag gacttgctgc tgccatagtt gaagctttaa caggaatagt tgtaatacct
    24421 ggaatgaaac caatcagtga aggtatagaa atagttggag atatagcaat agttttagca
    24481 ggagcattcc cattagtgtt tgttataaca aaggtattta ataaaccatt aatggcttta
    24541 ggaaaagttc ttggaatgaa tgatgtatca gcagcaggcc ttgttgcaag tttagctaac
    24601 tgtataccaa tgtttggtat gatgaaagat atggacaata gaggaaaaat aataaatgta
    24661 gcattctctg tatcagcagc attcgtattt ggagaccact taggatttac ggcaggattt
    24721 aattctaata tgataacacc aatgatagtt gctaaattag ttggtggtat aacagcagta
    24781 ttgttagcta tggttatcgc aaataagaca ctaaggaagg agaatgtata agatggatat
    24841 atcaaatata gataaaaatt taattgaaac attagtaaga caaataatag aagaaaaaat
    24901 aagtggaact aaagacacag tagattttgt tagaaataaa gacattagtg gaataacttc
    24961 tataaagtta ccaactgtaa aagttagtga atctgataga ttagatacag ggaatccaag
    25021 tgatgtagtt tacacaaaag atttatttac attagaggaa agcccaagat taggttgtgg
    25081 tatgatggaa atgaaagaaa caacttttga ttggactcta aactatgatg aaatagatta
    25141 tgtaatagat ggaactttag atataataat agatggaaga aaggtttctg cttcttcagg
    25201 tgaattaata ttcataccaa aaggaagtaa gatacaattt tctgtacctg attatgctag
    25261 atttatatat gtaacatatc cggcagattg ggcatctcaa aattaataaa aatcaagtta
    25321 agttaataat aatcaaataa ataatttaaa atacaaactc ttaaataaac acacatattt
    25381 aaaaaagcta tgaattaaaa ttcatagctt tttttataaa gaatgttata ttttagacgt
    25441 attttttcta tatttactgg gagacatatt atatttttta ttaaataatc taataaaatg
    25501 ctctacattt ggataaccaa tactatttta ctgataatct tttccttatc actaatctta
    25561 cattttaaaa tattattttt actcgtaata ttatcataag aatttgtttc ttatttaaaa
    25621 ctaattagaa ttataaagtt tattgccatg ttattaataa aataaatcca gttaaatcga
    25681 gtgctagtta aagtaataag aaacttttac aatggtttcc tttatcgatt gaaataaaag
    25741 ttaatatggt ataatatcta aagagagaat cttatcaatt agaatatgca tagaaacaag
    25801 tggcttaaaa ggagacgtta tatggaagag atagtaaaga aggaacaagc aagtatgaat
    25861 gatatttttt tgtatcttaa agctaaatca aactcattat ataaatttgt aatattatat
    25921 aatgagttta tcaataaacc taaagattat ggaactgggg aacttataaa catggttgaa
    25981 gtacatatgc taacagcgat tgaagaaaat ccaggaataa gtccaactca gttagctaaa
    26041 atatggaaca gaactaaagg tgcaatatct caaactatat ctaaattgga gaaaaagggt
    26101 tatataacta aagaaaagaa agaaggaaat gcaaaaacta tattactata tccaaccaca
    26161 tcaggagtag agttaagtaa ggcacataaa ctatatgaca ccatagatgt tacaagaaca
    26221 tttgaatact taatcaagga gtgtacatta gaggagatag atagttttta taaagtaatt
    26281 gatttgttta ttaatttatt agaagaatct ccgcaggaaa ataatgattc tgaaaaagtg
    26341 tagctaagat tgaagttgaa taaagagaaa tagatattat agaagtagta cttaagtaca
    26401 cattatataa aaatataata ggtataggat taaaaagttt agtggtttaa agtttaacca
    26461 ctaaactttt ttaatgtcta taatctacta ttatttaatt tactaaaaat aaaaaaactc
    26521 attatattaa gatgaaaata gaaaaataac tagaaaaata ctataatata tcagcttaat
    26581 acagaaaaaa gttaaaaata ttgagaaaaa taaaaatgaa aacgttttat attgacatag
    26641 agaaaaagta gtgataccat ttaaatatag tttagtagct aaactatatt taaattataa
    26701 taactatttt agggggatgt tatgaattta aaacaattaa gtgaaaaata cgaagaatat
    26761 gtaattcaaa tgagaagaga atttcataag tatccagaat tatcttttaa agaaataaga
    26821 acaacccaaa gaataaggga agaattacaa aaaatagggg tcccttttgt atctattgaa
    26881 ccaaatatcg taatagctac tatacaaggc aaaaatgaag gtaagacaat tgcaattaga
    26941 ggagatatag atgcgcttcc aatgcaagaa gattcagatg ttgaatacaa atcagaatat
    27001 gatggaataa tgcattcttg tggacatgat gctcatggag ctatgttact aggacttgca
    27061 catatgctaa atgatataag aaaagattta aaaggtagag tattattagt ttttcaagca
    27121 gctgaagaag ttggtggcgg acacaaggag ataatacagt actttaagga aaatggagga
    27181 ccagatagat taatagctac tcatgtttgg tccgatattg aagctggtaa aatttctgtt
    27241 gaaccaggac ctcgtatggc tggtgggtct ggttggagaa tagacataac aggtagagga
    27301 ggtcatggtt ctagaccaga tcaaagcata gatcctataa agccagcttg tgatatagtt
    27361 ttaaaagtat cttctatacc agttaatcat ataagtgtac tagaacaatg tgttgtacat
    27421 gcatgtgcaa tacatggagg aactacaata ggaaatgtta ttccaaacaa tgcagaggta
    27481 ataggtggat atagatattt tactgaagaa tcaggcaaaa aagtgtttga cattataaag
    27541 caaatgagta atggtgttgg acaagcttat ggagtggatg tgaaggttac tcatactgga
    27601 ggcataggac ctgttatcaa tgatgaagaa gcagttaaga tggctaaaga aatagtttct
    27661 aatatagatg gattggagtt agattcttat gagccaatat gtgcatcaga ttgttatggt
    27721 gaaatattaa aagagtattc tggtttttat tgttatctag gggtaggtaa caaagaaaaa
    27781 ggaataatat atgcacaaca ccatcctaag tacaatatag atgaagatac attaaagtta
    27841 ggttgcgaat ttatggctaa atatgcagtt gaatttttaa ataaaaataa taactaaaat
    27901 aaatcgaatt ttataaatgg gggggatgaa aatgcaaggt gcattaaagg aaaataagaa
    27961 aaaaagtggt ggaggatttt taggatggat tgaaagagtt gggaataaaa tgccacatcc
    28021 tctagcatta tttacatata ttacattgat agtagtagca ttatctgcaa tagcaagctt
    28081 tttaggtttt tcagcaacaa gtcctgctac aggaaagtta ataaaagtta tgaacttaat
    28141 aagtgcaaaa ggtttagtac tatttatgca agagtttgtt aagaacttcc aaaacttccc
    28201 tgtgcttggg gtagttttag ttatggcagt tgccacagga gtttgtgaaa agacaggatt
    28261 tttttcaact gccataaaaa tgagtttatc aaaagttaag gggaatgcgg ttgtatttat
    28321 aatagctttt ataggagtat ttgctaatca agctggtgat gctgcttttg tattagtacc
    28381 aactcttgca ggagcaatat tttatggatt aaatagaaat ccattagctg gtatattctg
    28441 tggatttgca tcagttggtg gtggatttgc aacagctgta attcctgggg gatgggacgt
    28501 tacgctaacg ccaataactg tatcagctgc tcaaactatt caacctgcat ttgatatgac
    28561 attattagct agttattatg cactatttgt atctgcgttt atagtagcta cagtattaac
    28621 aatagtaacg gttaaattta tagaacctaa attaggtaag tatactggta aaccagaagg
    28681 tatagatgat aatcaaggtt ttgaggtcac aaaagaacaa gcaaaagcag caaagttagc
    28741 aggttttaca gttttagctt atgtagcgtt attaattgct ttgtgtatac cagctaatag
    28801 ttttttaaga agtccagaag gttctttaat atttggagca ccacttatgt cttgcttaca
    28861 gttttttata gtaatactat tcttcttgcc agggattgtg tatggaatga gggttggtaa
    28921 aatcaagact gtaaaagact taaatgatat attatgtcaa tcaatggcag caatggcacc
    28981 gtttatcgta cttgcaatag ttataggtca attcttaacg ctattctcag tgtctaattt
    29041 agcacaggta ctagctataa aaggtggagc attactcaac tcattgccac taccaccaca
    29101 agttataata ctgctattct tagtactagt agcgtttata aatatatttg ttataagcgg
    29161 aagtacaaaa tggatgatat ttggaccagt atttgttccg atgttaatgc aattaaacat
    29221 acatccagca tttactcagt ttgtatatag attaggagat tctgtaacta atcatttatc
    29281 accattaaat gcattcttta taatactttt agcaacagtc caaaaatacg ataaaaatgc
    29341 aggtatggga actatattct cagctatgat accttacact ataggttact ttgtagtact
    29401 tgcagtttta gtagtagttt ggatgacaat aggtatacca gtaggaatag gtggacaagt
    29461 ttggttataa aacatattta ataattttgg gaggaaagaa aatggaaaca actgaaataa
    29521 gatacaattt tgatgaagta atagatagaa gtggaacaaa ctgtcttaaa tatgatctta
    29581 agagtcaatt tatgccagaa tctccagaag atgcactacc actatgggta gcagacatgg
    29641 attttgcatg tgctcctgag ataattaatg caatgcatga aagaatagac agaaaaatat
    29701 ttggatatag ctctcaacaa actgatgatt attatgaagc tgtatgtgga tggtttaaga
    29761 gaagatttga ttggaaaata gaaaaagaga atatattctt tagcccaggg gttgtgcctg
    29821 cgttaggatt tttcatacaa atattaacac aaaagggtga tggtatcata atagaaaagc
    29881 ctgtatacta tccttttgaa gataaaataa ctaataatga aagaaaagta gttaatagtc
    29941 ctttaaagtt tgaagatggt cattattcta tggattatga tggatttgaa gaattagcta
    30001 aagatgaaaa taataaggtg tttatattat gtagtcctca taatccagtt ggtagagtat
    30061 ggaaaaaaga agaacttaga aaaatagtag acatatgtaa gaaatatgat ttgtggataa
    30121 tatctgatga aatacattgt gatattattc gtaaaagtga aaagcatata ccattagaag
    30181 tagcatgtcc tgattataaa gatagaataa taacttgtac agctccaagt aagagtttta
    30241 acttagctgg tatgcaatta tcaaatatag taatcaataa taaggaatta caagaaaaat
    30301 ggatgcttga aacagattct aaattagcta tattcctacc taatccattc gcaatagttg
    30361 ctactaaagt tgcttataat gattgtgaag attggatgaa tcaagtaaat gagtatatag
    30421 atggtaatat taaatatgta gaagattttg ttaatgagta tatgcctaaa gtaaagataa
    30481 taaaatctca aggaacttac ttaatttggt tagactttag agggtatgga ttagatgcaa
    30541 aacagcttga atatgtaatg cttaacaagg cgaaggtatt gctagatgaa ggatacatat
    30601 tcggagaaga aggagcagga tttgaaagaa taaatgttgc ttcgcctaga acaattatag
    30661 aagagtgtat gaatagaata aaatgtgcat ttgaaggatt ataatagtaa tatctaaata
    30721 atatatttgt gtattttaaa aaaagactat gttaaaaaaa tatttcaaag catagtcttt
    30781 taaagtttta tgatgagttt aaaaaatttc aaaacgttac tattattgat aatcttcact
    30841 tatatgttta tattagaaat aattccacaa tatgctcatt ttctaaagtt gcacttttat
    30901 acaccttgct tcaccaatat catgtgtaac caaaatagta gacttattct cattcttaat
    30961 aatactataa acatcatcac aaactaataa tctagtctga taatcaagag ctgaaaaagg
    31021 ttcatctaaa agtagaatat ctggatttac agaaagtgtt ctaataagcg caactctttg
    31081 tctcatacct ccagataatt cttttggata catacttcta aaatcccata atccataagt
    31141 ctttaaaagg ctttcaactc tatctaaaga ttttttatct tttttgcctt gaatttccaa
    31201 tccaatagtt atattatcca ttatatttct ccattcaagc aaattatctt tttgaaacat
    31261 atatccaata tttccagtaa tctttatatc accactagtc ggttttaaaa gattagtaag
    31321 aatatttaat attgtagatt taccacttcc agaaggacca agcaaagtta atatttctcc
    31381 ttctttcaag ttaaaattga catcctttaa tacttcaagc tcgccttcct tagtgtagaa
    31441 gttcatattt atatttttaa cttctactat gttgtcattc attatgccac cccctataac
    31501 ttttagctcg tatatattat gttattaaca atatgaccta tttgtaccat ttttcataaa
    31561 tataatataa atattttgtt tatttacgta ttttcttagt aacttgtacg tataaattat
    31621 aataaataaa ttataaggta ggggataaat ttgagtaaca taataacgaa tttaatagag
    31681 tcagtattta ctaatccaat aactatattg tttttacttg caataactgt gtattctgtt
    31741 tttaaaataa tagaaaataa cagagtatat tcatttgtaa gttcaaaatt agacgaaatc
    31801 aatagagatt ataagacaag tgagttttat aaaaaggtaa gggatgacta ctatacatat
    31861 agtaaagaaa atccttatgc agacgtaaat ataacttcat ttatagagga agttgtatct
    31921 gatttaaagc ataataattt gccattgtta gaaaaaatta gaagtataaa aaattcatct
    31981 tcaataagta tattacttgg ggttttaggt acgtttgttg gtctttctac aatgctttta
    32041 tgtgttgata ctaaagacat tataaattca cttccttcaa ctattagtag tatgcaaact
    32101 gcatttacaa ctagtatatt tggagttgta ttttctctaa ttataggtta ctttactaag
    32161 ataaaagatt gtgaacatgt tttgatacaa ataatgttaa aatctgaaaa tttactaaca
    32221 tctgaaataa ctcattttaa gtcagaaaga atggatttaa aagtagaaga ggtaaaaaat
    32281 acaataaaac aaataagtaa gtctatagaa gctattgaaa aatttgataa aatatctaaa
    32341 gatttaaatg actttaatga tgaatttatc agtggtatag aagctttaaa aagcctttta
    32401 gaaggttctc aaagttctat taagacattt gaccagagcg taagaaaatt agataaacag
    32461 tttaatattt taaatataaa atttgtaaag ctttttgata aatatgataa tcaagataat
    32521 ataaataaag agattttatt tgatataaaa gaatcttcaa aaaatatata caatgccact
    32581 gaaagccaat tcaaaattag agattatata aaaaatataa atgcaggatt tgctctatat
    32641 gaaagaagtg ctcaagattt attaactaaa ttaatgaccc atgaaaataa aatctctcaa
    32701 aatcaaaaaa tccttttaga tgaaaagttt accttagatg actcaataaa aaatctctca
    32761 agtattattg aaaacttttc taatgaccta caagttaaac tggatatgat gtttgaaaat
    32821 tctttagata ttcaagataa gttagatgtt atgtttaata actcttttat gaatgatgaa
    32881 gttcctcttg atagtgagga attatttaag gatgatgtta ataatgtatt taatcctttt
    32941 agtgaagaaa tttatgagat agaggataaa gaaataaagg tcataggtga ggatgagtta
    33001 aatgaataat aaatatagaa gaactgttaa cagagaattt gaaaagagta gcttttggcc
    33061 aacatttaca gatttattat ctactgtatt gatggttgtg attcttatct tatttagctc
    33121 agaaagtatt tcaggttctg ttgaacaaga tttagctaag aatgtcaatg cttctgttga
    33181 agaaactttt aaaaaaagca aaatacctgt gaaggtagat aaatctaatg ggcaagtaac
    33241 ttttggtgaa aaaaccatgt ttgatgtaga tagtgatgtt ttaaaaccag aggctaaaga
    33301 aatgctaaaa atgtttgttc caaagtacat agagactatt tataaagatt atggtgacta
    33361 tatatccaaa atagttatag aaggtcatac agatgatgtg ggaagttata tatacaactt
    33421 agatttatcg caaagaagag catatagcgt tgctaaattt atagtgggag atgaaatagg
    33481 tgactataaa tacaaagata aagtgactaa gcatattatt gcaataggaa ggtcaaaagc
    33541 tgaactcatc aaaaatggtg acaatagcgt gaatagagat gcatctagaa gagtagaact
    33601 aaaatacgaa ataaatatta atcaaaataa ataattaaat ttttataaaa aaaaagaagg
    33661 cagttggtgt gagctttaag ccttctttaa ggaaaaagtt tatttatttt tgttgatact
    33721 taacatatta tagtttttag aagcagtttg caatatatta aacgtaaatg gtcgtttaaa
    33781 atgcataact ggatactact gatgtaaaat atttcatttt atataatata aaaattatac
    33841 agtgtcaatt ttctatatta ttgtactata atctattatt taaaatatta tgtaaaaaaa
    33901 tatcataaaa acgcatatca tgttataatt actagagtat tatgaatatg gaggcgtagt
    33961 ttgtgataga aataagtata aaaaataaaa aaaattctaa aattaaaact ttgatacttt
    34021 tattgatagt atctatatcg atttatttaa ctggttgtac tacatcagat aatggaaata
    34081 atcaatcaaa taataatgat tctttgtcta gtagtatatc aagtgataag aatgtaaaaa
    34141 ttcattttat tgacactgga aatagtgatg caatattgat tcaagatggt aaaactttta
    34201 cattgataga tgggggagat aatgatgatg agggattaat ggtagattac ctaaacaatc
    34261 aaggtgtaaa agatattaaa tatcttatag ctacccattc tcatgcagac catttaggtg
    34321 gacttgattc tgttgtcaaa aactttaata tagaaaatgt ttttgtatcg aatggttcag
    34381 cagaaacaaa gagttatagg gactttataa atgctttagc aaataagggt ttatccccaa
    34441 gtgtaccgct tgaaaataat aagttctatt tagaagattc ttattttgaa gttctaaata
    34501 caaatggggg agatactact aatgaacaat ctctagtcct tgtatatact aatgggaatg
    34561 ataaagtttt atttacaggt gatgctgaag aaggtaccga aaaagaaata ctacctaagc
    34621 ttgagaaagt agacctttta aaagtagctc atcatggttc tcgtagtagt tcatctcaag
    34681 agtttttaga taaagtaaat cctgaatatg cggttctttt agtaggcaag ggtaattcat
    34741 atggtcatcc acatcaggag acaatgaata aattgaaaaa aatgggagta aaagtacata
    34801 gaagtgatga gtgttcagat ataatatttg aatcaacagg tgatggagta tttacaagtt
    34861 gtaaagatgg aagttataat aaaggtgtta gagaagatgg aaactctgga aaatcaaaca
    34921 gtaatacaag taaaaatgat tcttttaaaa ataaacaaaa tacattcaat tcagaagaaa
    34981 tagtgtattt tactccaaaa ggtaagagtt atcactctat aaaaaattgt agtgggcttt
    35041 caagaagtaa aaaaatatta tcaggcacaa ttgaagaaag taagaaaaat gacccatgtg
    35101 atatatgtta tggaaaataa caaaaaaaca accaatttat ctataaagct tctgaacttt
    35161 aaatcattta aaaaataaaa taaatgcata aaaaaaataa gctataatca atagataata
    35221 catttcatct atacgaatag cttatttttt taagtatttt ttaatagcta tttaataact
    35281 ctaaatacac cagttacaat acctacaatt tcaacttctg agtcattgag cataattggt
    35341 tccataaaat cattttctgg ctgaagtctt attaaatttc cttctttaaa aaacctctta
    35401 actgtagcag attcaccatt tataagtgca actactatct gagaatttaa ggccgtattt
    35461 tttttgtcta ctacaacgta atcttcatct aaaattcctg cattaatcat actttcgcct
    35521 tttactctta atacaaaatt gttactgccc tttactaaac tggcagggaa tggaatgtat
    35581 tcctctatgt tttgttgagc taaaatgggt tctccagctg ttatttgacc tactaaagga
    35641 agctctatta tttcttgatt agctccagaa acatcattta ctttactacg ttctaaaact
    35701 tcaatagctc ttggtttagt agggtccttt cttatgtatc ccagtttttc aagtttattt
    35761 agatgagagt gcacagttga agtagatctt aaccctacag cagtacatat ttctcttaca
    35821 gcaggtggat aaccttttaa tgtgatttga gactttataa attccaatat caagacttgc
    35881 ttttcagtta gatctaaata cataacatca ctcatttcca aacatatttt gtttttattt
    35941 ccatacactt tatattttta attataacat aatccattta tatctgcaaa catttgttcc
    36001 tgatttttaa tcgtcctcaa tccattcaaa attcttgact atatcacgtt ttacattttt
    36061 cttatgagct gcataaagct gagtagttgt gacattatca tggtcaagta agttttgaac
    36121 atcatatata gaaaatcctg tttggattag agaagttgca gccgttgctc ttaatttatg
    36181 gggactatat ccattgtctc tagtagtatc aagtggaata gatgtatatt tttttaccag
    36241 tgttcttata gcctttgctg taattctctt attttgaaga gacaaaaata aagcatcttt
    36301 ttcttcatca tttaatagtt catctcttgt tctttcgttt tgcaaataat cttttattac
    36361 atgttcacaa gtatgattta taggcattaa aacttctttc ccacgttttc tatatatttt
    36421 aaattcacct cttgaaaaat taaaagagga gatattgagt tctctaagct catttaatct
    36481 aagcccgtat gtaacaaata atgctagtat agctttgtca cgtaatttag tttttctcca
    36541 atataccttt tctttctctg taagcccttc acctgtttct actgattcaa gcatcttagc
    36601 aacttcatct atctcaaggc gcttaatagc atctggctga ggtttaggaa gttttattgg
    36661 attgaatcca tcagttatat tattatctat ctgagagttt ctgtataaga atttaaatag
    36721 agtagagatg gatgattttt ttcttgctaa cgctctattg ttattttcaa aaatcaatgt
    36781 gtttttttct gtatttttgt aatatctact gcaataatca cctaagaata aatttatatc
    36841 tcttgatttt attgtattaa aatcatcttc tgtaatatct tttacacaat ctgcatttgt
    36901 taattctttg gtttcaatca aatatgaaca aaaaaaatta atatcttcta aataagcaag
    36961 tcgtgtagaa actgcaacag aaccttttaa atatataaaa taatccttca taaatacagg
    37021 aagtttattt tccaaaagta gacatttttc tatgagttca ttttctttga taacaatatt
    37081 atcaggttta tctgatttat tatgtacttt tataattttc ttatcattat tcaaaattaa
    37141 acctcctaaa actaaaatat aaatctttta tattattttt tgatatttat accaagtgag
    37201 tataaatatt taatttaatc attttttaca tttttaagag attagcagtg ctttagatga
    37261 aattatcact aactattccc taaatccaca tttaaagact agttgaattt aagtactctt
    37321 tttttatttc tcaatatatt tatagaaata ttttaaaata tattgagaaa ttttttatgc
    37381 tctactaaaa tcttttttat ataatatttt tatatatttc taacgaaacc aaatattagt
    37441 aaaatatgta tttatttaac taaagttttt aaatgggctg actaagctta aaatatagga
    37501 tattatgaga gttactattg aaaaaataaa ccctattgcc ataaagaaac ttataaatat
    37561 gttatactta actattaaac tggatatatt caagttatca aatccaaata agtatgtaat
    37621 tgaacttatt acaaaaataa ctataactat aaagctcaga ccatttaagc tattttttaa
    37681 atcagcacta ctaagtgaca tatgtgtaga tatagaatac attaaaaaca taaatataaa
    37741 atacttcaat gaatatacac taggtgttaa caacattatt cttataaaat tggaaatatc
    37801 acctaaaata atgtgtataa aaccatttat atttaaatta ttaatcatac ttacatataa
    37861 ctttaaatct atattttgag tcactttcat ataactatct ggcagtaata tcttaaaaat
    37921 taaaataatc attattgttc cacctataat aggtgcaatt cctataaaga aatttcctat
    37981 actttgataa agattatctt ttttatagct atgacttaca tatcctagta taccatcatt
    38041 tttactttct attggtctga aaaacttaac tgaatttatt ttatgcatga aaatcaagca
    38101 cattatcaaa tgactcaatt catgaacaaa cgttcctata gctccagtta caataattcc
    38161 agtttttcca aaagatgaaa agataaattt attatttaga ttttctgtaa tgctaaaaat
    38221 caatccaaat acaataaata ttcctatgaa acaaaaaata tttattacac ttatttttaa
    38281 aatttcgatt atataaatca taagttaagt cctcatgtaa aagtatttaa ctactctact
    38341 tcatatccat tacagcttaa aattttacaa gcatcttcta tatctttttc ttttactaag
    38401 atatagtctg tatcatacgt agaaattgca aatatgctta ttttgttgtc tgctaacaaa
    38461 ctacttaact tagataaaat tcctacaagt gaaaagtcta aaggacctag tatcttaagt
    38521 attctccact ccttttctga cttaacatca gaaggaatat tattatttaa acatacaaca
    38581 cttaattcat catcagttct agttatagag taaaacttct ctgtatctat ccatgttggt
    38641 attttactgt cattatttaa tctacatact gcatactctt ctaccaataa ttttaatttc
    38701 ataatgtata tttcctccaa tcttatttgt ttacttaaga ttatacatta aaagttacat
    38761 aaaataaaac atataaagta aaaaaggttc actatagtgt cctatttcta tagccaacct
    38821 ttaaaactta attaaattct aataaagaga tatattatct aggttcaatt ataagtttaa
    38881 tggctgtttt cttatcacct tcgatttcta tatcagtaaa tgcgggaatg caaactaaat
    38941 ccattccact aggtgcaaca aaacctcttg ctattgcaat tgattttatc gcttgattta
    39001 gagctccagc tcctatagct tgtatttctg caacgcctcg ttctcttagt actccagcca
    39061 aggctccagc tacagaatta ggattagatt ttgatgaaac ttttagtacc tccatttcaa
    39121 catctccttg ttaatttgtt aaagtatcca ttacctaaag tactaaatca taattgccat
    39181 ttactaatta caatcatcat ttaaagtata cttttataaa aatatacttc tataatcgtt
    39241 ttaagaaaac ctttattttt tttaaccatt ttattatgaa aaaactacaa atttctttat
    39301 attctataag cattttccaa tatttctaaa ttttttatat ctttgagcta taatttcctc
    39361 tttatccata tctttgtaat gatttaatct ttctaaaata taagctttta tttcttttga
    39421 aacagcttct acatcttttt gtgctcctcc aagaggttct tttataatct tatctattat
    39481 ttttaaatca tataaatcat tacttgtaag cttcataaca tcacaagctt ctttagcttt
    39541 agatgaatct ttaaatagaa tactagaaag cccttctgga gaaatcacag aataaactga
    39601 atgttctagc atacaaacat catttcctac tccgatacct aaagctcctc cactaccacc
    39661 ttcaccaatt acaaaagtga ttactggaac agaaagttta ctcatttcaa gtaagtttct
    39721 tgcaatagct tctccttgtc ctctttcttc tgcttctaaa ccacaaaaag ctcctgatgt
    39781 atcaataaac gttattatag gtctattaaa tttttcagct tgtttcataa gtcttaatgc
    39841 ttttctgtat ccttctggat gaggcattcc aaaatttctt tttatatttt cttttgtatc
    39901 tcttcctttt tggtgtccaa ctatagtaac attaaacttt cctatctgac caatccctcc
    39961 aaccatagaa gggtcatctt taaaaagcct atctccatga agttctaaaa aattagaaca
    40021 aatatgttct atgtattcca atgtcgtagg tctctttata tctctactta gtgttacctt
    40081 ttcatatgct gatagatgtg aaaaagcatc ttccttaagc ttttccaatt tctcattaag
    40141 tgaatttatt ttatcactta aatctatatt atttgcctta gaaatagtta ttaattgttt
    40201 aatatcattt tctaagactt ttattttttc actattattt tcaatcaaaa cagttcctcc
    40261 tttatgccct atttcttgtg catagcaaga atttgatata ataattcctt catttttctt
    40321 ctatctacta tcatgtcaat aaaacctttt tcaagtaaaa actcagaagt ttgaaatcct
    40381 tcaggaagtt tttgatttat tgtttgctct attactcttg gacctgcaaa acctatcaac
    40441 gtatttggct cagaaattat aatatcacca agcatagcaa aactagcagt tactcctcca
    40501 gtagtagggt cagttagaac tgatatatat ggcaatgatt tttcttcaag cttagaaagt
    40561 gcttgagaag tctttgccat ctgcataaga gataccatac cttcttgcat tctagctcct
    40621 cctgaagcag aacaaataat tataggtaaa ttattttcag cagcatactc tattgaataa
    40681 gttatttttt ccccaacaat agaacccata cttcccatca taaaatcagg attcattaca
    40741 caaataactg ttttaattcc atttattctt ccataacctg tgacaacagc ttctgactct
    40801 tttgtttttt ccttataaga gtctactttt tctttgtatt taggaaaatt tagtggattt
    40861 tgaaattcta catcagaatt aaactccata aaagttcctt tatcaatcaa taaatttatt
    40921 ctttttcttg ctcttaagcc ataatgtctt gaacattttg gacatacaaa tgaattttct
    40981 tctatgtcct ttctaaatac atgactatca caacctttgc aataggtcca aaatttatca
    41041 tctacactat tctttttaaa gttgtcatct aaagaaatcg taacatactt agattctttt
    41101 gatgataaaa atttttttat cataatctat ttactcctta taatgtagca aattctttct
    41161 tcataaagac atcttctata aatttggtat gatgagtatc ttctctaaac tcctcacttg
    41221 ttacaagtgc cttttgaaat tctacatttg tattaatacc ttctacaata atttcgtcta
    41281 gagctctata tattctactt atacattctt ctctagtttc agcccaacat attaacttac
    41341 ctatcataga atcatataaa ggtggtatag tatatcctgc atatacaaaa gtatcaaatc
    41401 taacaccaaa acctccaggt aaatttaatg attctatctt acctggtgat ggagcaaaat
    41461 tctttgatga atcttctgca tttattctac attcaatagc atgacctcta aaagtaatat
    41521 cttcttgttt aaaaggaatt ttatccccat tagctataga tatttgaagt tttataaggt
    41581 caagatttgt taccatctct gtaactggat gttcaacttg tattcttgta ttcatctcta
    41641 taaaataaaa gtttttatct ttgtcaacta taaactcaat agtacctgca ccgatataac
    41701 ctactccttt tactatatct actgcaattt tatgaagttt ttgtctttct tcatctgaaa
    41761 ggtatggact taaagattct tctattactt tttgatttcg cctttgcata gaacaatctc
    41821 tatcacctaa atgtattgca tttgaatgtt tatctccaaa tacttgtact tctatatgtc
    41881 taggattttc tataaacttt tccatatata aatcactatt agaaaatgct gctagagctt
    41941 ctgaacttgc catatcaaaa tttgaaaaaa gttcttcttc ttttctaacg attctcatac
    42001 cttttccacc gcctccactt gaggctttta tcataacagg atatccaatt tctcttgcaa
    42061 cttctaaagc ttcttcctta gttttcaaaa cagattctga accaggaaca acagggatat
    42121 tcaattccat catagtttct cttgctctag atttatctcc catcaaaatc atttgttctc
    42181 ttgttggacc tataaaaata atattattat cttcacattg ctttgcaaat tcaggattct
    42241 ctgataagaa tccaaagcct ggatgaatag catcacactt taatttaaga gcaagataga
    42301 ttatattttc tatattatta taactcttac ttatattatt tggtcctata caaatagatt
    42361 catcagctat atatctatga aaacaatctt tatctatttc agaatagata gcaacagttt
    42421 ttatacctag ttctttacat gttctgatga ttctaactgc tatatcgcct ctatttgcaa
    42481 ctagtatttt ttttatcaac ttaaatacct cctactacaa tggttttatc ttaaataaag
    42541 gttgattata ttctactagt tcttcattac taactaatac ttcaattatt tcacctttaa
    42601 tttcgcttgt tatctcattc ataagcttca ttgcttctaa tatacataaa gtgtcgcctt
    42661 cttcaactac atcgccaact ttaacaaagc tatctgcatc tggacttgga gaatcataga
    42721 atgttcccat caaaggagct actactactg atagattctc attagatgtg acaggtgtat
    42781 cgtttacaac tggcttttct aaagaaagat ccactactga ttcttgttgt acacttaaaa
    42841 caggtgaact tgtagattga gcttttttag atacttctaa tcttaaatcg ctactttcaa
    42901 gttttacata ttctaagtta gttgagtcta tggcctttaa taattcctta atttcattga
    42961 tgttcatttt gttaccccct tgtattatta cctggtgcta atattatatc acaaaaaaca
    43021 aatttataaa agaaaaagtg ctgactagag aatcaacaca tcaatatatc ttttaatttt
    43081 aaattaacaa ccagtaactt atatttgttt taattttcca ttattaatat aaattataag
    43141 tataaaatga ataaaattca atattttttg caaaatacaa gagaatacaa attaatacct
    43201 cgggaatttt taagacaaat accaatatta tgcaatttag acttcgcttt ctttcaaatt
    43261 agtatcattt tctttatttt tacataggat gtactttaat ttagctatca aaacattact
    43321 atataggtta aaagttatta gaattgcagg aattataaag taaactctag cacctataaa
    43381 accaaaaact actgtaataa gtattaatct tttatttaaa gtaaaaaaag gtatattaaa
    43441 aatacttttg tcggatgttt ctggagtata agaatatata actccaaaaa tcaaagtaag
    43501 catacaaagt ggaagtagtg tataacttga aattattccc agaaatccta tactttcaaa
    43561 atatttgcta gcactatttg tataaacaaa catgtatcca tttaaatatg cgcatataca
    43621 tgatataatg ccaaaagttc cccctaagga ttgagtgtac caattaaaaa atttagattc
    43681 catatacctc acctcctaaa actgtatata taatgatatg catttaaata gtaaaatata
    43741 tctttatatg tttctgaata aaattttgtt aatttcatta atttatgttt aagaaatttg
    43801 aagtatagtg tagtatatat taatgtagat gaagttttat agtacttatt tcatagtgct
    43861 taatcaagtt atcttattat ggattcaaaa gttgtttgta gggttttata ttttgttgag
    43921 ctgaaaaatt tattataaat gaagggatgt tttcgctttg gtagatacat tagaatttgg
    43981 tcttaaaatt ttatttttta tattatcaat aatttggatg ggtaaaataa tgattttaag
    44041 aactgataag caaatagtaa tcaatccttt gcttattggt atatctgcag tccttgtgat
    44101 gttgcatacc tcacagagta acattgaatt ttttggatta gatgtccaat atataagaat
    44161 agttttgtat ataatatatt cactgataat tttaatagga atatgggcta ctaacagaag
    44221 aaatggaatt ttttagataa tctatttcta taaaatttaa aggttgctag taacaaagca
    44281 ctaatattag cgttctgtta ctaacaacct ttattttgat tatacactta aattgaaaat
    44341 agaatgattg aataagttga catattattt tacattgaca gtgattagaa cttcttgaga
    44401 tgtatttaaa tcatttttgt cgcttagtat aacttttacc acagcttgac ctgttttatt
    44461 acctttaatt atcatatctt cacatgaaag tacactctta tcacttacag atacaataaa
    44521 gtctaaatct tttggaagat tatcagtaac ttcccagtat ttataataac tatcaatttt
    44581 cttcgtaatt atttttctca aatcaatact ttcacctttt gctatatcaa cattatgata
    44641 tttagagtcc agtttatact gattatcttt tgtatcaata aaatttaaat cgcaatttac
    44701 atccacacca ttatgagaaa gagaatttac tacatcgtca tcaatcaaat aattattggc
    44761 aagatttatt gaatccatat cttttagact taaagcttcc ttaggaacct cttttatatt
    44821 attattccaa aaaataaaat tgtctacatt ttcaagatat gatattcctt ttaaatttga
    44881 aatgctctca ccagttgcat aaaattctcc atctaaagcc tttatattgc ttatagtcat
    44941 ctcatctggg tttatatatc tttttacaac tgctcttaaa tttgcatcag gaaaaatatc
    45001 tttaagttga ttttcgctca aaaccttatc atttgaattt attggactta aagctgctgc
    45061 agaaataata aatggtatta aattagctaa attcatagta ccctcccaca taaatttttt
    45121 attataataa tttaatttct atgagagggt tctgtttcct tgttatatat acctactttc
    45181 gtacacttta ttaatataaa ttaaaataaa tttctactat taaacttata aacatattac
    45241 tagttacgtt ttttagcttc acatctaaat attcgcatac cttgtgacat aaatttgtct
    45301 tcatactctg ttgtaacatt attttcatac tcactatttc ctaaatcaag agaaatattc
    45361 tttaatagcc aattatttgc agcaatttca tttaaactaa actcgaataa tttttcatta
    45421 tctgttttaa agtgaatttc gccatcatct tccaaaaccc tattgtacat ttctaaaaat
    45481 ccagaatgag tgagtcttct tttactccat ctatttttag gccatgggtc acagaaattt
    45541 atataaattc tcgataattc tttagcttca aagtaatcca aaatattgct tacatctccc
    45601 cataaaaata gtatattatt caaattagaa gcatctgctt tttcaacagc ttttaaaaga
    45661 acttcttcct taagttccat ggcaatataa tttatgtcag ggttctgctt agctaaagtt
    45721 gttataaact taccttttcc agtcccaaat tcaacatgta ttggattatc atttctaaac
    45781 tttaaattcc acttgccttt taatttgtct atatcgtctc ttaaaacata ttttgtataa
    45841 cttaaaagct tttcatctgc accttttttt cgtcttcttc tcacaataat tctccttaca
    45901 tataaaaaat tagtaaaata catttttatt atacattatc tcaccaaaaa ataaaaagaa
    45961 ctctcttaaa atagaaattt atttctataa agagagttct aaacttacta aaaacttaat
    46021 cttagtggca ccctgaacaa ccagagcatc ctcctccacc ttgatctttc atattttcag
    46081 gaataactct aaatccaaat cctgtatctt ctattattat atctccatac tgactatatt
    46141 catcagagct cattacaaat tgtaattctc caacttcata agttaagtca tcttcttttt
    46201 tctcatctaa agctaatcca aaagaaggac ctccacaacc aacacctgca aagtatactc
    46261 ttatattatt aggtttatct tgattgtctt ttaagatact ttttaaagta tcaatagctg
    46321 tttctggtaa agtaattttc atcaaatcat tccctttcaa atattttatt aaaacaatat
    46381 ctattatata cccatacaag gtacataatc aattagtttt tgcatatttt actaaaactt
    46441 ctacctaata ttacctaagg ctttaaaata ttttatataa tattttatta ccttaaataa
    46501 ataccctat
//
LOCUS       DAMKPG010000020        46092 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMKPG010000020 DAMKPG010000000
VERSION     DAMKPG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46092
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.022"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            73..954
                     /locus_tag="PC621_001232"
     CDS             73..954
                     /locus_tag="PC621_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF4108401.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKKGVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1267..2046)
                     /gene="hisJ"
                     /locus_tag="PC621_001233"
     CDS             complement(1267..2046)
                     /gene="hisJ"
                     /locus_tag="PC621_001233"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF4108402.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(2586..2936)
                     /locus_tag="PC621_001234"
     CDS             complement(2586..2936)
                     /locus_tag="PC621_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF4108403.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2952..3368)
                     /locus_tag="PC621_001235"
     CDS             complement(2952..3368)
                     /locus_tag="PC621_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF4108404.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3387..3578)
                     /locus_tag="PC621_001236"
     CDS             complement(3387..3578)
                     /locus_tag="PC621_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF4108405.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3729..4544)
                     /locus_tag="PC621_001237"
     CDS             complement(3729..4544)
                     /locus_tag="PC621_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF4108406.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4591..5529)
                     /locus_tag="PC621_001238"
     CDS             complement(4591..5529)
                     /locus_tag="PC621_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4108407.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5526..6398)
                     /locus_tag="PC621_001239"
     CDS             complement(5526..6398)
                     /locus_tag="PC621_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4108408.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6423..8351)
                     /locus_tag="PC621_001240"
     CDS             complement(6423..8351)
                     /locus_tag="PC621_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF4108409.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8463..8840)
                     /gene="gcvH"
                     /locus_tag="PC621_001241"
     CDS             complement(8463..8840)
                     /gene="gcvH"
                     /locus_tag="PC621_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF4108410.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8927..11053)
                     /gene="cdhC"
                     /locus_tag="PC621_001242"
     CDS             complement(8927..11053)
                     /gene="cdhC"
                     /locus_tag="PC621_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF4108411.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11110..11916)
                     /locus_tag="PC621_001243"
     CDS             complement(11110..11916)
                     /locus_tag="PC621_001243"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF4108412.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11966..13333)
                     /locus_tag="PC621_001244"
     CDS             complement(11966..13333)
                     /locus_tag="PC621_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF4108413.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13364..14308)
                     /locus_tag="PC621_001245"
     CDS             complement(13364..14308)
                     /locus_tag="PC621_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF4108414.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14328..15098)
                     /locus_tag="PC621_001246"
     CDS             complement(14328..15098)
                     /locus_tag="PC621_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4108415.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15154..16539)
                     /gene="lpdA"
                     /locus_tag="PC621_001247"
     CDS             complement(15154..16539)
                     /gene="lpdA"
                     /locus_tag="PC621_001247"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF4108416.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16583..17464)
                     /locus_tag="PC621_001248"
     CDS             complement(16583..17464)
                     /locus_tag="PC621_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF4108417.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17500..18174)
                     /locus_tag="PC621_001249"
     CDS             complement(17500..18174)
                     /locus_tag="PC621_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF4108418.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18211..19083)
                     /locus_tag="PC621_001250"
     CDS             complement(18211..19083)
                     /locus_tag="PC621_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF4108419.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19175..19807)
                     /locus_tag="PC621_001251"
     CDS             complement(19175..19807)
                     /locus_tag="PC621_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF4108420.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19912..21588)
                     /locus_tag="PC621_001252"
     CDS             complement(19912..21588)
                     /locus_tag="PC621_001252"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF4108421.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21837..22616)
                     /locus_tag="PC621_001253"
     CDS             complement(21837..22616)
                     /locus_tag="PC621_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4108422.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22926..24845)
                     /gene="cooS"
                     /locus_tag="PC621_001254"
     CDS             complement(22926..24845)
                     /gene="cooS"
                     /locus_tag="PC621_001254"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF4108423.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25452..26363)
                     /locus_tag="PC621_001255"
     CDS             complement(25452..26363)
                     /locus_tag="PC621_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF4108424.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26479..26601)
                     /locus_tag="PC621_001256"
                     /pseudo
     CDS             complement(26479..26601)
                     /locus_tag="PC621_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(26684..27826)
                     /locus_tag="PC621_001257"
     CDS             complement(26684..27826)
                     /locus_tag="PC621_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF4108425.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(27844..29598)
                     /locus_tag="PC621_001258"
     CDS             complement(27844..29598)
                     /locus_tag="PC621_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF4108426.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            29734..29940
                     /locus_tag="PC621_001259"
     CDS             29734..29940
                     /locus_tag="PC621_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108427.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(29945..30868)
                     /locus_tag="PC621_001260"
     CDS             complement(29945..30868)
                     /locus_tag="PC621_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108428.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(31122..32822)
                     /locus_tag="PC621_001261"
     CDS             complement(31122..32822)
                     /locus_tag="PC621_001261"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF4108429.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(33233..33646)
                     /locus_tag="PC621_001262"
     CDS             complement(33233..33646)
                     /locus_tag="PC621_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF4108430.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(33714..36092)
                     /locus_tag="PC621_001263"
     CDS             complement(33714..36092)
                     /locus_tag="PC621_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF4108431.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            36409..37860
                     /locus_tag="PC621_001264"
     CDS             36409..37860
                     /locus_tag="PC621_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF4108432.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(38064..40577)
                     /locus_tag="PC621_001265"
     CDS             complement(38064..40577)
                     /locus_tag="PC621_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF4108433.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(40800..41621)
                     /locus_tag="PC621_001266"
     CDS             complement(40800..41621)
                     /locus_tag="PC621_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4108434.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(42140..42616)
                     /locus_tag="PC621_001267"
     CDS             complement(42140..42616)
                     /locus_tag="PC621_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF4108435.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(42650..43228)
                     /locus_tag="PC621_001268"
     CDS             complement(42650..43228)
                     /locus_tag="PC621_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF4108436.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(43332..45968)
                     /locus_tag="PC621_001269"
     CDS             complement(43332..45968)
                     /locus_tag="PC621_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF4108437.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDT
                     LSNTSKIDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWG
                     SMEYFKKKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIE
                     AVIYGYAPMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRS
                     TIYNSKVTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKA
                     TDLYKKLDEIGTVAGHYYKGVE"
BASE COUNT        14037 a         8903 c         5110 g        18042 t
ORIGIN      
        1 ttgaaggtaa atttaaaggt actatatact ataaatatgt cgacacaata aatttaaaaa
       61 ggagaactaa atatgaaaaa aagattaata ataatgatat tgtctgttgt cttagtacta
      121 tcaagtatac ttacgatatt tgcatattca aatattaaat ataataataa taaattgata
      181 tattctaata tgatagataa aaaaactcaa aatagtgtaa aggaaatcct aaaagaaaat
      241 aaaattaatg aaaaagacat agatacattt attaaagcag tcaacaatta taataaattg
      301 caagttaaaa tacttcaaaa taatataaat atatctaaat ctggatattc ttcaatcaat
      361 gcaaaacaag ttccatataa cctagaaaaa ttgcaagata attgggtgaa aaaattccct
      421 gattatatgg atgtaaattg tagaataact gcttttagac tttttaaaga ttttataaat
      481 tcaaataaga aatttactgg agactctatt gatttgaatg tcgacttaga cactataatg
      541 aataataaag atgctaaatt tagcaccaaa gatgtagaaa aatttatcaa cttcttttct
      601 gcaataccag ctaaagatac agatgataca atcaaaattg cagaacaaat aaaaaatgaa
      661 tggaaaaaaa gaaaaatatc ttttaaaaat aataaaaata tatctattat aaatggattt
      721 cttcgttatc cagaaactaa aaacgtattt ataggtcata caggcatatg tattaaaact
      781 aaaaatggca ttctattttt agaaaaatat ggtgttacat caccttatca agttactaaa
      841 tttaaaaata aaaaaggtgt aaaaaattat atgtttaata gattgaagat gtcagaaggt
      901 gaaattgaat tgcctgaccc tataataatg gaaaacgata aacttatgaa ataataactt
      961 taaacgcaaa atagtattta taattactaa gtcaatataa tagagaaggt ctatagattt
     1021 gtgtaaatac aatcttatct acaatcttat ataagaatgt ttatataaat aagatagatt
     1081 aattctaagt cctattattc tattgaattt tattattatt ttacttagat tctattgtta
     1141 ttttacttag aacttattgt tattttattt aagttctatt actattttca atcatataaa
     1201 tttacaaaag actgtacata aatttatttt atacaaactt atctacagtc ttttgtgtga
     1261 atatatctaa ataatatcgt atttatttat ttcctctgca cagtgagaat cagaacctaa
     1321 aactaatgga atatcgtatt tttttaccaa ctctaataag tatccatcta tataaggctc
     1381 tttgcaatac tcttttctat ttccagaaac attataatca agttcataac ctttttcttt
     1441 aattaacttt attaagtctt caagaattgt atttccttta taatcgtatg gaaatacctg
     1501 attaaacttt ctaactaaat ttaaatgacc tattcttttt ggtttatgta tacctaaatc
     1561 tgaatttaca gcttttatca atgtttcata atacttatta taaacagctt caatacttcc
     1621 taatttgtcg attaaatttt gaaactcctc tacactgtaa tctacacaat aataatcatc
     1681 atcaatttta attatatgta ctgataaaag agagtcttct aattcctttc catatttatt
     1741 taaattctcc tttatccctt cttcgtatcc ttctataaaa tccacttccg acccaacatt
     1801 tatcttgatt ttaccctcat acttttcttt caacttcttg acatcattaa aatactttgg
     1861 taaatcttca atactcatac aactatcatt ttcaggagca gggtctctaa atccatttgc
     1921 atacggaaaa tgttctgtaa atgtcatttc tgttattcca acatccattg ctctttgtac
     1981 atatttttca aaatcatcct tacttccatg aggacaataa ggtgaatgta tatgaccatc
     2041 tttcataaat aattcactcc ttttttaaag aaaaaatcta atataaatgc ataaattttt
     2101 taacttcaaa ataatatttt taatcatgct aatttttatt atatctaata ttagagtaat
     2161 tttaaactgc cttttaagta tcttatcttt agtactattt tcaacttaac attaatatta
     2221 ttttttaact taacattaat actatcttta aatagtaata ctcaatatat aattaatcaa
     2281 ataactcaaa ttgtttgatg aagcttttgc agtatttaaa gaatatataa ttatttaaca
     2341 ttaaataatc caatcttgta ataaaattta ctcattaaag tatattacag aaaatatctc
     2401 aaaataaact ttttagttca tgatatactc ttttttgcat gaaattagat atatttttat
     2461 catttcaaca atgaaaggga ggcttacatc catcttgaga taacccctta gataaaattt
     2521 ttaataatta aacatcataa aatataaaaa tcgcttatat caaacgtata agcgattaat
     2581 tatatttaca tatgtttttt agcctctaac aacattttat tagcttcttc catatactct
     2641 atagcctttt caatactctt tgctgtttct tcttttccaa tagctctagc cttttcaacc
     2701 cactctctaa atccttcttc atgagtttca ttgtgattta tccaatgtac taaaagtatt
     2761 tttaaagttt tttcatcctt agactctgat gattcctcat gactatgttc atggtcgtgg
     2821 tcgtgagcat ggtcatgtgg atgttcatga catatatctc catgagtatg ttcgtggcag
     2881 tggtcatggt catgatgtcc atgatgatat cttacaatct ctttatcaaa aatcattagt
     2941 tattccccct gttattttat attaaactct ttttttataa ggtttacaga atcttttaca
     3001 agtagtgcta atggtttacc ttctaatcca actaactcca cattttctgg cataataggt
     3061 aaaagtatct taagtgcctc actgtctgca atagcttcac ttatattaca agtaacttct
     3121 cccatcatag aattaggtat tattacagaa ataggtgcaa ctattatatt tgctttttta
     3181 gctgatacta ctatagcatt ttcccctgta gctcctttat tagcatgagc tttcatcata
     3241 gaactagttg ctatagaatt agtccctagt gcataaattt ctacatatgt aggtaactct
     3301 tctctcaaag aagaaactat ctgggctcca atacctccac ccattccatc tatcactgct
     3361 attatcatat gaaattcccc tattaattaa ttttcttgta ttattatctt atggtccatc
     3421 aatcttattt ctttaattat cccatctatt atcttttgct ctcctaataa atccgttaaa
     3481 taaatctttc catcacaagg gtctatatta accacatttt ccataacctt ttctaactca
     3541 ttattacttt tacaaatgaa agctgatgat tcacacatat aaatctcctc ctaatatgat
     3601 ttttattaaa aatcaaaaaa agattccctt aggtatttca atactccaaa gaaaccttca
     3661 tgatttcagt ttttgtatat tattatacct taagtatatc actttaattt atgtaaagtc
     3721 aattaaagtt aacgcttttt attatactct tcattcttgt atttttcata tagttcttta
     3781 actaattgct tctcataatc agtcaaatcg ccctccacaa actcaacatt aaattgttca
     3841 gctaatccct ttactatgtt tttctgtaga atatctatgt ctatttttct gccaatttca
     3901 ttttctattc cacttgcttt tttagctgta aatttcatag ctctttcttt caattctggt
     3961 gtcttagttt ttattaattt aaacaatttt tctacatcaa aatccagtat tattgaacca
     4021 tgttgtaata aaacaccatc ttttctgctt tgagcacttc caattacctt cttattatta
     4081 atagtaactt catatgatgc atgtgcatta aaacatgcag ctgataaatt ttccctactt
     4141 attctctcgc ctctatttaa attatctgtt tctataccac ttagattcaa tcctttaact
     4201 agaccttcac ttatatatct ataagatagg ttgatgctct tatcgattaa aggattatct
     4261 tctcctataa ttatactata agttagctca ttatcatgta atacagctct tccacctgta
     4321 attcttctag tatagtctat attcatacat ctacacttat ctaaatctat ctcatcttcc
     4381 aatttctgaa aatatcctat actcaaacaa gctggttccc atgtataaaa tctaagagtt
     4441 ggcttattat gtcctttctt atatgcagta aatatagctt cgtcaattgc catattcatt
     4501 gctccttcat atgatttatt atgtataact ctccactgat tcatgattat tacgccccca
     4561 tttttagtat aaatatatcc tagaaatcct ctatagattt atttctctta ttgctaaatc
     4621 tatctcactt tgatttaaag gtcttgaata gttgtatata gtacttccgg gtctttcatt
     4681 atagtatggt ctattacaat ccttacaacc tcttatttca aaaggatagc ctttgtttat
     4741 gtcttttaaa atatcattgt caagctttat actatttaag tatccatttt taaacttaaa
     4801 atactctttt ggataacctt tatttatcat ataagacatt aactgtaccc ttctataact
     4861 ttctatgctt ggttgattta tcttttccat tttagttcct ctaactggag taaatgcaaa
     4921 taaactaatc gttacatcat tttcctttaa ataaatatat aaattgtata tatcttcatg
     4981 actttcaccc ataccaacta ttatatgtgt acttatttta ttgggatatg attttgacat
     5041 ttcagttata aattttaact tttcatcata gttatttcct tttatttttt catatagttc
     5101 tttatttgat gcatctattg ctattcctat tttatcaact cctactgaaa aaaacttctt
     5161 tatctgttca tcattttcta attttgcaga tagtgaaatt ggcatgtcta tcttaccact
     5221 tatatagttt ataaagtcta aaacagagtt atgtgcttct tcacttgcca ttgattgaat
     5281 acatattctc tttatatttt ttcctttata agcttttaaa gcatctaata tttcttcttt
     5341 tgaaaactct ggccaaatta ctcttgataa tttatctttt ctagtactac tttctattga
     5401 ttgagaacaa aaactgcatt tatttataca tttttctcca atcattatat atgctgtggt
     5461 aggaggtata tcgctttttt tgtttaatat cccaagctct attgctgtcc caactgatag
     5521 tctaatcata aaacgcaaca ctccttacac tcatttattg tcatattcat ttctatagcc
     5581 ttattctttg cagaccttga tggtaatact attctattga ctccacacat tatagataat
     5641 tggtctagct cttttctata cactcctctt ggtctcatac aacctagatt aatttcagta
     5701 tctggtaaat taattctagc ttcacataaa atatctgcta ccccttctaa ctcaggaggt
     5761 tctacatttt catactcagt cccttttgtt ggaataagga ctataaatgt aagcttatta
     5821 ggaggatttt tctgtaggta ttctataatt tcatattcac cctttatttg tcctcctttt
     5881 aaaccaatac atatatgtgg tgctacttct gtatgttctt gtattgtatt ataaacttca
     5941 atataatctt ctttactctt tttcatctta taaacttctc ttattgtctc atcatcaaac
     6001 actaaatcaa aagaaactat atctaaatac ttacacaact ctacaatgct atctttatcc
     6061 ataagaccta aatgagcatt caatctatat cctttctctt ttaagttttt aatggtatta
     6121 atatgagtat ttatagggac atctccctca taactacaac cgccactcaa tagaaagcta
     6181 gttatattcc ttgattgtac tttttcctca tattcttcta ttggcaccat attgtttaag
     6241 tagtgaccgt tgcaatgggc acatttcaaa ctacattgat tgcttgtagt actaagagct
     6301 agtgtctggt taggataagc aaactctatt ttatttccaa aattctttaa tttaatatca
     6361 aatgctattt tcattttttc agatagattc ttattcatag tttattcctt cttaagtaca
     6421 ttttactctt ctagtaattt ctttgctgtt gggaactgct ctatattcgt atgtggtaag
     6481 aaacatgcag aaataaattc atccatataa cttggatata cactaagttc aacataagtt
     6541 atttgattac ctatttcttc aagtttattt tttgcatctt tacttatgag agctaagtaa
     6601 gaaccaacaa gtgaactatt ccctatataa gtaaattttt ctctatcaat gtcaggaagt
     6661 aaccctataa ttattgagtt ttctatgtct aggttgtttc ctataccacc tgctatataa
     6721 accctatcta atatactaaa atccatacct aaactatcta caagagtgta tgcacctgaa
     6781 tatatagctc ctttagctct gatgaaatta tctatatcaa cttcattgac tactatatca
     6841 ttttctaaat caaattcttc tttaaatgct aatacatact ctcctatctc atgctcatta
     6901 aatctaactc tcttattatc taaatccctg tatattttac ctcttctatc tattacacct
     6961 ttagtaatca tttgacagat taaatcaatt ataccagaac cacatatacc aactggtgca
     7021 cattcatcaa taatctttaa tgttggctct aaagtttctt tatcaataat tactttctct
     7081 attgctcctg ctgatgccct cattccacag cttatccctc caccttcaaa agcaggtcct
     7141 gctgaacatg cacagctcat catataatct ttatttccaa atacaatctc tccattagtt
     7201 cctaaatcta taaataaaac attttcttca cttgaccata tccctgctga taatacacct
     7261 gctgttatat ctcctcctac ataacttgca acagatggag ctaagtacac atatgcactg
     7321 tcatttacaa aaagaccaac attttcaccc ataagttttg gagattttaa aaatggtggt
     7381 atgtaaggtt cttgtcttaa gaagtctgta tacacaccca agaataaact tgtcatagtt
     7441 gtattcccgg cagccactaa tgtaactaca tcttccttat ctattccatt tctttcatat
     7501 attgttttta atagtggatt tatagtttcc tcaactatag ctttatgaag tgtctctaat
     7561 ccatttttct tagttgaata tataattcta tttataacat ctgctccata ttttatctgt
     7621 gcatttccag aagatgcttt atctactacc tcttttgaat ataggtctac taaacaaaca
     7681 actactgaag ttgtaccaat atctattgca actccgtata aggaattttc tttattacct
     7741 tgttctatat ttatgattgt aagtttcttt tgtttttgaa catatgttat tgtaacctta
     7801 aaatcactct ttctaaatac agttggcatt tttctaagta tatccaatct aaaatctatt
     7861 tcattatatc ctaggctatt tctaacatat ctttccaatc tatctacatc acttatatta
     7921 tcatctaaat ttggttcttc catttctatg tatttctttt ttatatttgt tttaaattga
     7981 aggttatgtt cttcaattat ttttttagct ctttcaaaaa tttccctatc ctctttttta
     8041 ttactacctt ctatcttcat accatgcata gaagatgata ctttagaagg aacttcaatt
     8101 tctatatctg atattacctt tgtacaacaa gctagtatat aaccttgctc ccactcatca
     8161 tctgttatat gtcttgtttt ttctgtatca acttttccat tcaataattt tactttacac
     8221 ttcccacaag acacgtttcc attacaagga gcatctatga atatatctgc atttcttgca
     8281 acctctagta atatatcacc ctcattacaa taaacctctt tattgtttgg tgtaaaactc
     8341 acttttatca tgttatccct cccaatttgt tctatatatg ataaaaggaa agtatagcta
     8401 aatttgcgtg cactcaaatt ttactatact ttccccaatt ataataatat cttaatcttt
     8461 aattattcta atccagcctc gtattcttta tcacttaata acgtatcaag ttcagccaca
     8521 tcagctagtt ttacttttaa tatccagttt tcatatgggt cttcatttat acattctggc
     8581 tcatcttcta atttttcatt aacttccaat acctcaccat taacaggaga tattaaatca
     8641 gaagctactt tagaagactc aactactcca aaatctactc ccttagttac agtatcttct
     8701 acttctggtg tttcaacaaa caatatttct cctaattgat cttgagcata gtctgttata
     8761 cctatatata caacatctcc atcaattact tttacccact catgatcttt agagtatttt
     8821 aattctggta ataatttcat ttcgatagac ccccaatatt atattattga ttaaattact
     8881 taaatcatat taatattata tcatattatt ttaaattttc catcaattac attacacttt
     8941 ccatagctaa tgctggatga cctttttctt ctaagaaagc taatactcct tcagaatcgt
     9001 cagctatagt ttcatcacaa accatatcac agaagttttc tattccagtc atttctttaa
     9061 ctgtagcatt taatttatca gctacataat cttttaattc ttttggcatc caaactattc
     9121 tttctggtcc accttctgca taagcgaatt ttttagaaga tatgaaatgt cttccgtgtc
     9181 ccataaatcc tggagtttga actcctccac ctgtcataga agcaagttct ccgaaagtca
     9241 taccaactgg agtaacactt gcaaattctc tgttaactac aacaaatccg tttgcttctg
     9301 gcattatacc acagatacat tcgaagcatc cacaagaagt cattgggtct tctagtatag
     9361 aatataatgt aacagattca actgcacctt gagatatttg gtttacagtt tcatttactg
     9421 aattccatac acctgttcta tcatctaagc actcgccttt ttctattggt tgacaaggac
     9481 ctgttgggtc taattcttta gtagccttag catctaacca gctaactgct ccacaaagac
     9541 caagtctttc aggagtaaca acacaaacgt gagctggtgc aaatgattga cataagttac
     9601 aagaatagaa agtatcaaca ctttcatcaa ctaatgaagc taatctttca tctctagcat
     9661 tgtacttagc tattgcttct ccttttaatt cagacacttt ttcagcatct gtaattatag
     9721 ttatttcaca cttatcaact actgattcaa attcatctaa catcttagca tataaaactt
     9781 ctcctatatg ctcaagtcta aatcctttat caaatgcatc tttagagatt cttacccaag
     9841 ccatatctct ttgtccaacg tgcattacac cttctatata gttcaagaag tagtggaaac
     9901 gtctttcaag aactggctcg aaatcttctt gcatattttt accagctatt ttaactatta
     9961 cagcaagcgg taatcttaat acaccatctg catcaacttc atctatattt ggtcctatta
    10021 tttctatctt atggtcttca acttctgttg cttctttctt aacaacaagc tcccaagctt
    10081 cagttctgtt tccaccaaac tcagcaaaca tatcgctctt tctaattctc tcaccttcaa
    10141 atgctgctgc aaatgaaact gggataggta tttcagtaac ttttattttt attcctctag
    10201 cttctaatga agtagctact atcttatcgt aatctttttg agttaatagg ttcattggaa
    10261 cttcaagaac tgtttggtca gttataactg ggaatcctaa agctattgct ccagcacctg
    10321 cagatacaac taattcactt aatggtccaa atgcattaac aaatgcagga actctattag
    10381 ctgtgtactc taataatccg tttaaatcac caggagttaa tccaccgaat attaatgctg
    10441 ctcttactgc aacagataca acgtgtataa cagaagttac atcatatcct agaggtataa
    10501 ctcttagctc aagacccatt tttactccag cttctatagc ttggtctata actttaccaa
    10561 ctaagaaagt tagtaaacct ttagattggt aatcttttat aacttttgct gctgattctg
    10621 catctggaca ctcaccaaga acaactgcaa caccaggtat atctcctgta actaatggta
    10681 caccaagtga tctgattata gggtcagtta tatgacctgc acatggctca ctatatggag
    10741 catccatagt tgaatatttt aatgcttcaa ttacttctgc agctaaagcc gtagctaaac
    10801 cagcattaaa agcatgttct agtaaatgag ttctatttat taaagatttt actacttcta
    10861 aagcaccttc taagtcccct aaagtgttca tcttttgtcc tgttgcagca tatatacaag
    10921 gtaatgaata tgctgtatca gggaaagcaa ctttatgctc ttttccattc ttttctatag
    10981 cttcagctaa catagcttga gccgcaccta aagcttgttc tgaccctgta aagattatat
    11041 tatatagatt cattctattt ccccctttaa tatttacacc tattttttat taactatacc
    11101 ctaaaaaccc tagaaagcaa atccaccttc atttaactct aagaaagagt ctgcatataa
    11161 agatgccccg ttgattatgt cacaagattt tacagttttc attaattctg ggtcacatgg
    11221 attcattata gctgaactaa atccattagc cattgccatt gctaagaatg cactatctaa
    11281 aacaggtcta acatgcttag gacatccatt agatacgtta gataatcctc ctgtagttaa
    11341 aagtcccatc tcagtcatca tttttatagc ttctaaaact tctacttgtt tttcttgcat
    11401 accttttata actaagcata atgggtcaaa tagtatatca gttggctcca ttcctaacat
    11461 taagcctttt tctaatattt cttggcagta agccatacgc tcatcattat ctcttgggat
    11521 accttctttt gcacataaac ctataaccat tgcttcgtat tcagctgcta tatctattaa
    11581 atcaaatctt gagccagcat cagcagagtt tattattggt tttgcattag ttctatcata
    11641 aactttaagt ccagcttcga tagctttttt attagctgta tctaatgcta taggaacatt
    11701 gttgaactca gattgaagta atttgattcc ccatgtcatt atttcttcgc catctctttc
    11761 ggcaggtcct atattaaaat ctatatagtg tgctcctgct tctaattgct cttttgctct
    11821 ttttaagatt ggagctgggt ctctttctgc taatgctttt cttattgaag gtgatataca
    11881 atgtattctc tcacctataa tcataaattt ttccatttat aatgtccccc tttaaattta
    11941 atattaatag ttagttaata ttttattagt ttgtttggca tgctttttct tggtatcctt
    12001 ttaagaactt aggtaattcc atagattcct ctggtcctat aactacattc catcctggta
    12061 agttatcttc tatatcacct ttaagtactg caacttttcc tggaatgata aggtctttac
    12121 aattagttac ttcttctacc ttactttctt taatgaaagc agagattgag tttccaccaa
    12181 atttaccagc agcccaagat gtaagaactg aatatccacc tgcatctggt attactaacc
    12241 atactggaac ttttgatctt tctatatcac cagcaactat gaagtaagtt aatgcaaagt
    12301 caacagtaac taatactgga gaattttcat ctgggttgtt gattggataa atctttggtt
    12361 caactctcat tggtctttgt gggtcagtgt atatgttttg tcttagtgca aataatggta
    12421 atgccttagc ataactaata tcatctatta ctattataga accgtatttt atagtgaata
    12481 ttgatgataa agcaacttcc atcatagggt ttgaattaga taatctattt gcaaatacta
    12541 ttgaaggata tccaaatgtt ctatcttgct cttttaaagc tgttcttcta acttgtatag
    12601 cattagtgta agtatctttt atgttttctc ctgttacatc taatactaat tctttatatc
    12661 cagcggcttg aattaattca actgtttcat ataattcttc taagctacca gcttttacac
    12721 ctaatggtaa acttgctcct ttaactactt ctatcatatc tttgtagttc tctttagttg
    12781 ctccatatac cattggtcta gcatctttta atacttcaac agcttcttta actacttgtg
    12841 catcatcaca agctaaaata taagctactt ctaatccact gccttttatt ttatctatta
    12901 attttaaata cgcatcttta tctccacaat actctaaaac agccatttca actttcattt
    12961 gttcaccaat tcttacatag tcaacttttt tcatgttagc tattttagca tctactgctt
    13021 catcactcat acaagtacag aatgaaactg catatctatt tctacttact aatgtttttt
    13081 catgtctaaa taatactgtt tctccaccta gttcatactc agatgcacca gcaccaactt
    13141 ttaaagcttt cattaaaggt gcagtagcct ctgataattt tgctatagcg tcatcagaca
    13201 tatgtgggca tttacctact tctacagctc ctgaagcaac cttcatagag aaagccatac
    13261 aagttgggaa tccacaatcc ttacagtttt tctttggtgt taatttaaat atatctaaag
    13321 cttttaatgc cattattttt tccccctaac cttaacaatt taattaagct aatgcgttaa
    13381 ccaattcttt tatagtttct attgattttg gatgacgaag tataactgcg tttgaaccac
    13441 ctactaaaac acttgctgca gttgaaactt ccattgctat agttctttct tctggacaac
    13501 cccaatctgg ctcatcttct attggagcta tagcttcttt aacatgacct acttcaaaag
    13561 ctactggtgt tatgataggc atttgtaatg tcttatcatt ttgaccaaat gctgcaagtc
    13621 taattctatc catagtagat gcaacgtact catatccata accaactgct gaacatccaa
    13681 cattcataac tatattttca cctttaacgc ctagttgagt taacaatacg tttaattgtt
    13741 tagctaggtt tatatcaaca gaagattcag ctcctacttt atgtgcataa gccataccag
    13801 ctgatgctcc tactcctttg tagttatctt ctactgctga cataaataag cagttatgtc
    13861 catccaatgt ttgagctaat ttttcaaata atttaccatc tttttcatga tttcctgaac
    13921 cagctacaac taaaggtaat tttattgctt caattactgc cttagctaca tcagcacatt
    13981 catcaactga cttatctaat ccatttgggt cagaaccatc aaattttaaa caaataaaat
    14041 ctgcttgtgt attagcttca acatatttag cccactctac tggacaatta gctacatctt
    14101 tgtataattc cttatatgaa tcagtccagc tttcaggata aacgtctgat atttgtatac
    14161 ctatttttgg agaattacct acttctccat caaaactata aaaaggtaat acattttccc
    14221 cacctaattt aattgctttt tcccctattc ctacttcaac ttctgatatt tttccagaat
    14281 atttttgagt agacatttta aatgccatat ttttttcccc cttaagccta ttttaattct
    14341 aatttagcaa aaatttcttt catagccact ttagattttg aatcttcagg taaattaact
    14401 aaaggaatac ctgtagaatc atattcatat atcaattcat ccataggtac aacaccgatt
    14461 agctctaggt tatgcttttc aatttcttct ttaacaccat cattcataac cccatttgga
    14521 actttattta caatcaaaag tattcttcca acacttaact ttagttcctc agctaaatct
    14581 cttattctag caacagcttg tatgctacgt cttgagcaat cactaaccaa taaaagtgta
    14641 tcaacatgtc tagtgacttt tctacttaga tgttccatac cagcttcgtt gtccatgact
    14701 aagtatttgt aatgaccaga tattttgttt acttgttctc taagtatccc atttacaaaa
    14761 cagtaacatc cttcaccttc tgacctaccc attactaata agtcatatcc ttcgccttct
    14821 tcgattatag aatttaatct aaattgaaga tattgtgcct ttgtcatacc accagggaat
    14881 gcattgccta acttttctct ttgatttact tcttctctta tttctcctat tgttgcttca
    14941 acttcgatac ctaacacttc atttatgtta gcattggcat cagcatctac aactaatact
    15001 ggtccctttt tgtccttaac caaataatca attaaaagcc ctgtgagact tgttttacca
    15061 gttccacctt ttcctgcaac agctatattg tatcccattt taagacttcc cccttaaatc
    15121 tagccacaga gccttatgta ctctgtctgt ctattataat ttaggtacag aatgaacaca
    15181 ttctccatgt acatcatgaa gtgcttccat tagcccttca gataaacttg gatgtggatg
    15241 tattgcatcc cctacttgtt ctactgttaa tcctagatga acagcaagtg acaactctgt
    15301 caataaatct gtagcatgag gtcctactac tgctgcacct attatcttat cagtttcttt
    15361 gtcagctata acttttacaa atccttggaa atgtcctata gcttgtgctt ttccaagacc
    15421 tctaaaatca aattgaccta cattgtattc tacaccttca gcttcaagtt gcttttcagt
    15481 cttaccaaca ccagcaactt caggttcagt ataaacacat cttggaattg ctctataatc
    15541 tactacctta gtcttaccta aagcattttc aactgctacg ataccttctt tagaagcaac
    15601 atgtgctagg aaaggagtat ctattatatc gcctattgcg tatatacctt ctacattagt
    15661 ttcaagatgt tcattaacta ctacttttcc tctttccatt tcaattccta tatcttctac
    15721 tccagaattg tcaaggttag gtcttctacc aacacatact aatgcatatt gtgcttctat
    15781 aacctttcca ttagaaagag ttgcaaccgc cttaccatca actacttcac aagtttgtac
    15841 tccaatacct gtaataacct taattttatc tttcttaaat tgtctaagta gttgtttagc
    15901 tacatctttg tcttcattta atagtatttg gtcaaccatc tcaactatag ttacttctgt
    15961 tcctaaggct ctaaagaact gccctatctc acatcctata actccaccac ctactattaa
    16021 catagactca ggtatctctt caagtccaag aacttcatca ctagttatta caacttttcc
    16081 atcatatggg aacattcttg gtacaactgg aacagaacca tttgctagta ttatcttatc
    16141 tgcttttata gtttctactg ttccatcatc tttagttact tctatagtgt ttttatcaat
    16201 taattttcca aaaccattaa ctaagtttac tcctcttttt tcaaataaga actctatacc
    16261 acttattaat tggtttacaa ctttattttt tctttccatt atggcagtga agtttggctt
    16321 aactgtacca tctatttcta taccaaaatc ctttgcttct tttacagtat tcaatactcc
    16381 tgaagaagca agaagtgctt tagttggtat acatcctgca tttaagcaag ttcctccaac
    16441 tcttcttttt tcaataaccg ttacatctgc accaagcata gaagctttta tagctgctac
    16501 atatcctcct ggtccaccac ctactactac tatcttcatc cttaattccc ccaaacctaa
    16561 tataatctac aatcttgcaa tactataaac ctgcggcatc cagtatagca ggtatattct
    16621 cttctgcccc aattgccatt atatgaacac catcacataa atcttcttct tttaattgtt
    16681 tgataagttc ccctgccatt tttattcctt cacttaccca gttttctttt ccagctgctc
    16741 ttaatctttc gatttgttca tctggaacga atattcctgg tacatttgca gtcataaatt
    16801 tagccattcc tggtgatttt aaaggtacta ttcctgctaa taccttgcaa tccatatccc
    16861 ttgttaattc tctaaacttt ctcattgtat ttatgtcata aagtgcttgt gtttggaaaa
    16921 atttagcccc tgcttttatc ttcttttgca tttttaataa ttgaacttct attggagaat
    16981 attctggagt tactgatgct cccagataaa aatctggaga accttttaac ttattaccag
    17041 ccatgtccgt acctgccatt aaagtctcag cagtttgaag tatccccaca gaatctaaat
    17101 caaacactcc cttagcctgt ggatggtctc ctacgctagt atggtctcct gttagtgcca
    17161 acaaattgtt tatcccaaat acacctgctg acaacatttc tccttgtata gctattctat
    17221 ttctatctct ccctgttatt tgaataactg gctctaatcc agcatctttt aataatttac
    17281 atgtagcaag agaagttgct ctcataactg ccgattgaaa gtctgttaca tttgctgcat
    17341 gaactcttcc aactaacggc tttgcacatt ctattaaatg tgaaaggtca gtcccttttg
    17401 gaggagccat ttcagtagta actgcaaatt ttccactttc taatgtttct cttaataagc
    17461 tcatctctaa tccccctttt aaccttctaa aaaaaacttt tatgaatttg ctgctgcttc
    17521 tttctttttc tttgcactta aacttcttgg attattttgc ttagaatagt cttttggtgg
    17581 tcttatttct gctaagttat ctaattgtcc tatagcttct agtctttcat atatctttat
    17641 ccaagcacaa tcattctctg agtttacttc acacttacca tttttagctc caccacaagc
    17701 cccattcata agacctttag cacacatagt tactggacat atcccacctg tccaaccaag
    17761 ttcacagtct ccacaagcct tacaggcttc ttcatattct ccaactcttt gtacttcccc
    17821 tataaacaaa gtattgttgg ctggataaac tggcttatct tttaaattct taactattgt
    17881 ctgtgtacca tctccacatg ctaaagataa aactgcatca gcttccttag tctcttcttt
    17941 tagagccttt aaatcctttt tagactttaa aaggttacaa gcagggtcaa gcattacata
    18001 tcctaagatt tttttgcctt caccttcaag agtttccttc atttttaaaa cttcttcttc
    18061 cccaccactt ttacaagttg ctgcacattg attacaacca actaaaacca gcttgtcaaa
    18121 atcctttaag taccctagca cttcttctaa tggtttattt tcagaaatta tcatatctac
    18181 ttccccccta aaccttccac atatatatct ctatttattg ttttgtaatt tacaagcctt
    18241 aactacatgt tttgcaagta tagatgttgt aactgaacca acccccgctg gaactggagt
    18301 tatcattgat actttgtcta aaactgcatt tgtatcaaca tctccacata aatttccttc
    18361 ttcatcaaca tttataccaa cgtctataac taccgctcca tctttaacaa aactttcatc
    18421 aaccatctta gctctaccaa tagctgctat tagcacgtct gcctctgctg ctacacctga
    18481 tagattttta gtttttgaat gacatattgt aacagttgca tgttcactta ataaaagcat
    18541 tgatactggc tttccaacta ccattgatct tccaagaact gttaccttac ttccttttaa
    18601 atcaacatta tagtgtttta atatctcaac tactgctgta ggtgtacatg gtggaaatcc
    18661 acttttatcc ccttccatta ccttagcaga gtttattgga ctaaaacaat ctacgtcttt
    18721 ttccggtgca ataacatatt ttataacttc ttcatttaag tgttttggaa gaggtctaaa
    18781 acataatata ccattaacat ttttgtcatc attaactctc tttaatacat caatatattc
    18841 ctcttgtgtt atatcctcag ctaactctaa cacttctgtt gttataccta tgttttgaca
    18901 tctctttagt gctcctcttt cataagataa atcatcactt cttgccccaa ctctaactat
    18961 tgttagttta ggatttatac cttctttaac tagtaaatcg acttctttta ttaattcttc
    19021 actaatttta tcagctacag gttttccttt tataatttgt cctttagttg acattccttc
    19081 catacattca caactcccct acaagcaaac ttaaattaat ttattctata taccacatat
    19141 taataacata gtatctactt tcgtcattta aaaattattt tccaagagct ttttcaactt
    19201 tggcataaac ttcatcacaa atttttacac catcttcaac taacttatct acttctattt
    19261 ttaccttatt aacatattct ttatcttgta tagaatttat atttataact acatttaatt
    19321 gtccaccaag tatagcagct cttaaacact gaactcctac accaacatca cttatagcaa
    19381 gtcttgaacc tttatcaact aaatcttcat gtaacttaat agcttcatag caaactctta
    19441 ctatgtttaa tggtacttca caagcaactt ttaatgcctt ttccattgtt tctgctttta
    19501 ttcttttttc ttcttctgtt tctttaggaa gcccgtaagc ttttgataat ggtaagaaac
    19561 attctgcatc atcatctatc atttttaata aatctttttc taactttcct gccttactta
    19621 atatctcttt tacgctttct tcatactcag catacttctt cttccctatt gtaaggttac
    19681 ataccatact acctaaagcc atacctattg cacctactag tgcagcagct cccccaccac
    19741 ctggtactgc ttctttggaa gatagtactt ctacaaaatc aacacaagtt ttatctgcta
    19801 ttttcatgat aatcctcctt gaaaggatgt aggaaaatat ctcaaattat tcgagatatt
    19861 ttacgaacta ttttaagata tctcctactc tttatttata aattaatttc cttagaataa
    19921 tccagctatt actccatttt catcaacatc tattttttct gctgctggaa cttttggtaa
    19981 tcctggcatc ttcattattt ctccagtaag tgcaactata aatccagctc ctgctgaaat
    20041 tgttgcttgt cttacagtta ttctaaatcc tgttggtcta cctaatttag tttggtcatc
    20101 agttaaagaa tattgagtct tagccataca tactggaact ttagtaaatc caagtttttc
    20161 taagttagct atttccttat cagcttcagg agtataatct acaccatctg ctccataaat
    20221 cttagtagct atagcattta tcttgtcttt tatagataaa tcatcttcat agcagaatct
    20281 aaagttattt tcttcttcat ccaataatct taaaacttct ttagctactg caattccacc
    20341 ttctccacct tttgcccata cttcagaaag agcaacatta acacctaatt ccttacactt
    20401 gtcttcaaca agtttaagtt cagcttcagt atcaagtggg aatctattga ttgcaacaac
    20461 tgctggtaat ccatatactt gagttatgtt ttcaacatgt tttaataagt ttggaagtcc
    20521 tttttcaaga gcttctaggt tttcgttatt taagtctgct ttagcaactc caccattgta
    20581 ttttaatgct cttacagtag caactattat aacagcatct ggttttaagt ttgccattct
    20641 acattttata tctaagaatt tttctgctcc aaggtcagca ccgaaaccag cctcagttat
    20701 tacatagtct gcaaagtgca tagccattct tgtagctata acagagttac atccatgagc
    20761 tatatttgcg aatggtccac cgtgtacaaa tgatggtgtt ccttcaagag tttgaactag
    20821 gtttggtttt aaagcatctt ttaataatgc agccatagca ccattagctt ttaattgtct
    20881 agctgttaca ggttcacctt catagctgta tccaactatt atgtttccta atctttcttt
    20941 taaatcagat atatcacttg ccaaacagaa cgctgccatt atttcagaag caacagttat
    21001 atcgaatcca tcttgtctaa ctgctccatc acctttttta cccataccat caacgatatt
    21061 tctaagttgt ctatcgttca tgtccacaca tcttctccaa gttattttct tagggtctat
    21121 tctaagtgca tttccttggt gaatatggtt atcaagcata gcagctaaaa gattgttagc
    21181 agctcctata gcgtgaaaat ctcctgtgaa gtgtaagttt atatcttcca taggaacaac
    21241 ttgtgcatat cctccaccag ctgctccacc tttaacacca aatactggtc ctagagatgg
    21301 ttctcttaaa gcaactaata catttttgtc tagtttagca aatgcatcag caacacctat
    21361 agtagtagta gtttttcctt cacctgctgg agttggattt atagctgtag ttagtattaa
    21421 cttagctttt ttaccagtct ctctttttag tagattgtag tcaacttttg ctttgtattt
    21481 accataaagt tctacatcat cttcacctag tcctaatttt tttgcaacct ctctaatatc
    21541 ttgaggctta gcttcttgag ctatttcaat gtcagattta aatcccataa ctgaatctcc
    21601 tcccacaacc taaatagatt taaaatataa tttttgtaaa aattgtatta atatgtaaaa
    21661 attatatttt ataagataat tatacatgta aaattactta aaaactagct actaagctat
    21721 taaaatattc aaacttaata gctagttatt tatctaatta cttacttatt acctatctat
    21781 tgcctatgtc taacctttaa ccaacttaaa atcccaaatc aaaatttcta tctattttat
    21841 ttatccttaa gtgacattaa tttatctttt attgctttaa tctgttcttt cgttgtttca
    21901 ctagaatcat atggagattg attagctcta tcagaatcaa taacatcttg gttatagtaa
    21961 ataaatccta aagattctcc atcttctaag ttttggatta taaattcttc atcttcctta
    22021 tttctaattt tatttcctac tacaaatact ttatttacac ctatatcatg tccaagtttc
    22081 ttaacttttc tataagtctg tagacttctc tctcctggtt ctacaactac tataaaagca
    22141 tctacacctt gggctgttcc tcttcctaag tgttcaattc ccgcttccat atccataacc
    22201 actacatctt tattttgtag tattaaatgt gagcagagtc tcttcaaaag aacatgctct
    22261 ggacatacac atccagtacc accagaatct acggttccca atgtaagtaa tcttactcca
    22321 ttgtattctt tacagaaatt ttcagggata tcgtctactt taggattcat cttaaacatt
    22381 tttccaaatg aacctacaga agcagctgtt ctatctgaaa ctaatttttt catttctgat
    22441 ataggtacta ttgattcata tacttctttt ggaaaaccta gtgctaaagc taaattagca
    22501 tcagggtcgg catcaacagc aacaactctg tatccgtctt cagcaaacat cctagagagc
    22561 attgaagaaa aagtcgtttt acctactcca cctttacctg ttattgctat cttcataatc
    22621 cctaccacct tttattacta tttgcttttt agtagttaat tttacagtta aatatttttt
    22681 atactctata tttttataca ctaaaaataa actacagcta gtccttacag gaaccaatta
    22741 aatttaccct actagctttt caaagcagca tgtatagttt tattattctt taatattgtt
    22801 tatattttat ttattcagta tattcattat atttcgatgt tctttacttt aatatttgac
    22861 tttattcaac tttactcaac cagaaacttc ttataaaaac gtttattcta taacttcact
    22921 attttttaag cttcagcctt ttctgccatt ttttcttcaa ctaatgcttc aaagtgaaca
    22981 cgttttttct ctatatcttc tattattgta gctgctaact cttttggatt tgtatttaca
    23041 gtaaatgtag ctccaacttt atcttttaac tcactagtta atatatccac tgctcttgaa
    23101 gaaccagcta ttggaggcat tattcctaaa taagtgtcta taccagaagc tacaacatag
    23161 catcctatag caactgcttt ttctgacatc cattctggag ctatacctac aactggaagg
    23221 tcacacatat ccatatctaa gtaattagct gctgctccaa ctaaatctag tatacgactt
    23281 atatcaacac aagaacccat atgtagtact ggtgggattc ctactaattt acagacagtt
    23341 gcaagacctt tacctgcata tttttcagcc gcatcagatt ccattaaacc aaacttagct
    23401 gctgctgaag ctccacaacc tgtagtaact actattatgt cgtttttgat taattctttc
    23461 attattgtta cgtgagcctc atttgaaact cctttagcat tgttacatcc aactacacca
    23521 gctgctcctc ttaatactcc agcttttaga cagtctgtta atggcttaac tgttcctgct
    23581 gggtctatat gagagtttac aactctgtct aattgattta ctactgcttc tacactatac
    23641 ccaactgttg cgcctgattt taattcaggt ataagtactt ttgatttatc tctattttta
    23701 aagtttaaaa tagcttcttt aactattgct ttagcatcat caagtgcttg ttcttctcta
    23761 aattcaatat aagttgaacc tgttatctta gcttttggag aagttgttac aaatttagta
    23821 tggtaacaat ctgcaactct agctaatgca gggaatatac attgaacgtc aactattaca
    23881 gcttcaactg ctccagttat tattgcaagt tcttgttgat ggaagtctcc tgctattttt
    23941 acaccatgtc tcattgtaat ctcattacct gtacaacaca tacctgctaa gtttattcca
    24001 tcagcaccaa cttctttagc taaagctact aagtctggct cttctgatgc taaaactatc
    24061 atttctgata atgctggctc atgtccatgt aatattatgt taactttatt ttcttctaat
    24121 actgctaaat tagcctctgt tcttcttggc actggagttc caaataagat atcgcttaga
    24181 cgagtaccta tcatagaacc accccaacca tcagccatag aagttctaag actcatgtgt
    24241 attaaactat ctatatcacc agtacatcct atatgagtag agtgcattat tgttgcaatt
    24301 tctctatcta tagctcttgg ctctatagcg tattctttcc atattttttg tcttggttct
    24361 ggtgcatttt ttataaatct taatgtccca aaaggttttc caaattccat taatgcaatt
    24421 tctgccactt catgtgctac atcatagata tctctacctt ctgtctctac atcccattct
    24481 ttagctaaag taattaactt agcttcgtct cttattttat agtttccatt tggatcagct
    24541 aatgctaatg tatgtgctat atctctagca tggtctgaat gggcagctgt cccaccagca
    24601 accattcttg caaagtttct acctactata gtatgctcat ctgctccaca taaccccctt
    24661 ggagtcttag ggcttattct acatggtccc attccacata ttctacagca aacaccttga
    24721 agaccaaaac cacattgtgc cttaaagcct tcttttctat catacattgt ttctacgcct
    24781 tcttctctag ctttagctat catcttctgg ctgtttaaat caattgttaa cattttttca
    24841 tccatattcc tatccccttt ttttgatttt taaaatcccc caggtgactg acttcatgtc
    24901 ataaaataat aaaataaatg ctttctaagt tgattatata ctaattttca gtgctttgct
    24961 ttagatttaa atattttttt aacaaaatta gataatacat aaaaatttta ttttctacac
    25021 ttaatttaag ttgattttat cgcttgtttt tggctatttg ttaatatcta aacctacttt
    25081 tttgatactt cagtttaatt aaattatgtt ttgatttttt taaatcaaat ttgaaatttt
    25141 tctagtcatt tttatattat tgcagattgt ttgtgtattt attatctttc actactgttt
    25201 ggacttatta cagattttta ctccctaaac ttaattagta aaaacttctt agaatagcat
    25261 tttttggact aatttttttg aattttttta tttttcctac tttaatatta attatagctt
    25321 caaattgaac atttgtatat attcatttga agctataatt attttttata taaaactttt
    25381 ttaatacagc attaatttag acattttgac tattttttct actttaattc atttatttca
    25441 agcgatttat cctatttcat atttttagct acaatagccc catatgcaat agaatataat
    25501 ttagattcat gtgaatcagc tcttgacact aaaactatag gagcttttgc acccataatt
    25561 atacctgctg atttagagtc tgccatatat gtaaaggctt tccctattcc attgccaacc
    25621 tctattgttg gaaccaatat gatatctaca tctccagata cttcactttt aaaccctttt
    25681 atctcactag cttcttttga aacagctaaa tcaaaagcca gaggaccttc tacaattaca
    25741 ttttcaccaa attctccttc tttacaagct tttgctaaca tatctgcatc tacagttgct
    25801 tgcatcttag gatttacttt ctctttagct gccaaacatg caacttttac agtttccatt
    25861 cctaaagctt ttgcagcttt tatagaattt tttaatatct ttgccttttg ttcataatca
    25921 ggagctatat tcatacctcc atctgtagtt ataagtaatt tatggtattt ttctaactca
    25981 tataccataa catgacttaa taaactatca gttctaagac catagtcttt atttaacact
    26041 tcttttaata tgacagatgt atctaataat cctttcatta caaagtcggc ttctttactt
    26101 gatactaatt taactgctat ttctgcacac tctttgatat catccttatc cactattttg
    26161 ataccactta aatcaaatcc tatttctttt gatatttctc ttattttgcc ttcatttcca
    26221 accaatatag gagtaattat atttctttct acagcatcct ttattgccat taaaacttct
    26281 ttatcatgag ctgctgctac agagagtaca attttctctc ctcccttaag ttgttctaaa
    26341 atatcatcta atttttttat caaaagtaac ccctccctta tatatattta actaaataat
    26401 accatatttg ctataccttt tctcaattta ttttttatgt tataaagaat ttatatgact
    26461 aaaagacagt ttaatatttt attctgggat ttcatcaata gctcttaaaa gctttgcagg
    26521 aacaccacct acaatagtat tttttgttac aactgctcca gcagcgataa ttgcattatc
    26581 tccaatagta actcctggaa ctcttcatac aaaaaagagg cttatctcta ttttgagata
    26641 gccccactta accgataaaa ttaaatacaa cctaacaata tccttactta tttattccaa
    26701 gttctcttag acaagcaata gtaatttcat cttgtggatt taattcataa gctctttcta
    26761 tatagtaagt tgcatcatca atatcaccat tatttaaata agccattcct aaattgcaaa
    26821 gtatttctgg gtcttccttt atcttagccg cttgttcaaa atactctatt gctttatcta
    26881 aattattctt ataagcctca caaagcccca attccactat agtatccacc tgattaggct
    26941 ttattatcaa tatcttctca agatacatct tagcctgctc tatttcgccc atacccttat
    27001 atccaagtgc tatcataaac agcaagttcc accaatccat atgttcttct tctaatggaa
    27061 gtaacttctc taatccttcc ttaaacttac cttggaatac aagtgtatat ccttcttcat
    27121 attgaacttt aaagtccatc ttgcctagat tctcttgtac ctcagctact aaatcagcat
    27181 ctaaccctag ccttaatgct tcttcccaag tcagcttagt ctttaaatat tgaccttgat
    27241 tataataatg gtagcctaag tgataataac ctaatgcaaa attctcatca acatcaatta
    27301 ccttttcaag cttagccatg gcttcaagta agaatgcatt cattgcctta tcatccatat
    27361 ctttttgata ctgtttagct atctcttggc aaacaatcgc atagttatac attgcatcta
    27421 aatcatctgg atacattgtt ataaatgctt ttatatatat taaagaatct gtatgttcac
    27481 caatctcaaa gtattttctc gacatatatc ccatatacga tttcaaatcc aaatcaatat
    27541 cttttgataa agcatttaaa aacttctcat actctgcatt gtttttaaac ttactatcta
    27601 ttccaattat atatatcatt gcatctgcta tagacattga atttaactta tcttgagcag
    27661 tcttttcttt tatccctttt actaatacct cattttttat aggtacatct aatccgcctt
    27721 ttggcacttt atagcttttt aatttaaact ctccaccatg ttttatagtt ataaaagcta
    27781 attcttctgc tttttttagc aaatattttt ctatcttaaa atccatttta tcctcctaaa
    27841 tacttaaatc tctactttat gagttttaga tttctcaaat atttttttat catcatactc
    27901 aaataagact acattttcta cttctcttat ttctgtattt atatcatttt ttaaatccac
    27961 aacattaaat ctaaatggct caaaatgaac atattttatt atatgtttag ctggtttatc
    28021 aacaaaacta ggtaaatatg ttgataagtt atcattatct tgtaaaaata catggcatct
    28081 attcttaaaa tcatccacat ctagcttatt aaaaaactgt ggtatatttg atgttttacc
    28141 cagtgatata tagtcatatt ttaatatatc attgaataca tcattatcta tgttgatttt
    28201 ttcattatag aaatccatca atattttata taactgattc tttccttgtg ccaaatcaaa
    28261 gtagccattt tcatcaaaat acttggcaaa aacctcaaag aatttaaaag gactttgttt
    28321 ataaaatctg cctataatat atctcataga taacacaaag tttttagaat tgtagtatct
    28381 ctccagtata tcttctatat cctttagctt caaagtttct tcatagctta tataattatt
    28441 gtataaaaac tcatatggag cataatcctt atatttaaaa ccatgctcat ctgcaacttt
    28501 tctcatacct gtccctttaa tcatctttaa aaagcctaat tgcagatgct ctatacctag
    28561 attaaataca tcattaaaag aattttcaaa gctcttataa tcttcataag gcagtcctgc
    28621 aattaagtct aagtgctgat gtatatttct ataagatgct acagtttgta caacatgaga
    28681 caacttttta aaatcatccc ttctacctac tgcatcaagt gttttttcat tcgttgactg
    28741 aacccctatt tcaaattgaa aaagaccttc tttacaatca gccaaaaact caagcatctt
    28801 atcatctatc aaatacgctg taacttcaaa atgataagtt gtatatccat tatcattttt
    28861 cataaggaaa tccatgattt ccatagccac ttttctattt gcattgaatg tcctatctat
    28921 aaactttatc tgtgatactc ttgcatctat taaagctttt aaatcactct ttatcctgtc
    28981 taaactaaaa taccttagac ctggaatagt agatgataaa cagtactggc agttaaaagg
    29041 acaacctctt gatgattcat aatacactat tctattttca tattcttttg gattcaagtt
    29101 ttcataagga ctaggaatct catccaaatc ttgaagtaat tccataggct tattcactat
    29161 tatctcattg ccttgtctat atacaagtcc atttacatca tttattttca tctttccttg
    29221 caagtgaagt actaagtctc taaaaattaa ttctccctct ccataaagta tataatccac
    29281 aaaatcatac tttttcattg cctcataact atcataactc acttccggtc cgcccaaagc
    29341 tattttagta ttaggtctaa ttttttttag attatcacaa agtttaacta tatctcccac
    29401 attccagata tatgttgaga ataatattat atcatattca tttttatata tatcctttaa
    29461 aatatagtcc aaatcgttgt ttatcgtgta ttctttcata ttaacttcta tcaaatctct
    29521 tacaaattct tttagatatc ttatagccaa atttgtatga ataaacttgg aatttaatgt
    29581 tgttaataaa attttcaaat atttctcacc tctttttaag attatactgt ctttttgtac
    29641 cccttatagg ttaaaatagt atatgtaaag tctaaataat atctatataa ttaatttatt
    29701 aatttcaagt aatgtgagga aaggaagttt tttatggaat taattgaaaa agtatctatc
    29761 aactctttat caaaaagaga tttactactt attataaagt ctcttgaata cactaatgaa
    29821 aatactcaaa ttagtgattt cattgacctt aggaataata tagtaaagga attatgtttt
    29881 ttaactgaca ctaaggaaaa agatttttta gattatctag aaaaaaatag cacactctaa
    29941 taacctaaac ctttttaaac tttaatatat aaatacctga tttagaattt atttttatgc
    30001 ttaatatatt atcattaaag gttttcgaca taactgttat ttgattaaat atagggtcta
    30061 ttatagtatt accttcttca agtcttataa gacttcttgg gtctatgctt ttattatatg
    30121 ccttaactcc aattttatat tttctcaatt taacaacata ttcggacata gaaaaaataa
    30181 aatctatatt atcttcattt aatttactat cttcacccaa aatggataat atagattttt
    30241 ttatgctatt aattccttca ttttctacag ttctatcttc catatataca tcatatagaa
    30301 catttaaaac atttaataaa atactttctt catcataatc ttcagatttc aaatcaatgt
    30361 acttatcaat taattgtatt gctttaatcc ttgccatctg aaactttaca acatctgatt
    30421 tgtctaattc tatagaaaat cctattatct tgtctttaat actctgtagt aattcgacat
    30481 actctatttt actattattt ataagattgt gcataatgga attatacact acagaaccta
    30541 ataaataatc aaataaaagt tcttcttttt taaaataaat aacatacttt tttattagtt
    30601 taataacctc atcttcgctt ttttgaatat tagtatttga taaaattagt ggtagtaaaa
    30661 tatttaaaaa attgatattt cctatttttt ctccataaaa tctatagtaa ataagttcat
    30721 ctctcagtct attttcgtct atatacacac atctttgcgt tttatcatct attaaggatt
    30781 ttgtatttat tatagttaaa tctttaagag ccctacttgc actactaaaa gaagatgtgt
    30841 agactttttt tagtaattcg tcattcataa atatatcctt tctgaaaaat tgaatgtaaa
    30901 taaattgtta ttaaaaaaaa agagacagcc cgtttaagaa gctgtcatat atagggggat
    30961 agtagtgtac aaaaatcatt gtaatctact atctatatat tattatacga tactactttt
    31021 ttatatgtaa agcatcagtt ttcgacatcc ctatatttta cagttataca aattgcgtac
    31081 taagttcaat tttattcttt ttatttcaac attttatcat gctacattct ttccggagcg
    31141 tgcattccca acaatttcag agaattttct aaagtttgtt ttgttgctaa aactaatgca
    31201 agtcttgctt ttcttatttc caaattttct actaaaattg gattgtcatg ataaaattta
    31261 ttaaacgctt gagctaaatc taacataaat ctagtcacta tatgtggctc atttttcttt
    31321 agagcaagta aaatattctt attgaatgat tctataactc taagaacttc tgcactatct
    31381 tcatcagcta atagactata atctatatca gaagtcactt ctacttctgc ttttcttaaa
    31441 actgcacagc atctagcatg tgtatactga acatatggtc cagtctcacc atcaaaacta
    31501 agcgttcttt cccaagagaa ggtatagtct ttaattctac tgttagataa ctcttggaat
    31561 actactgccc caacacctac ttgcttagat atttcatcca catttttagc atttggattt
    31621 ttagctagta taatttcctt agttttatct atagcttgat ttaaagcatc ttctaaaaat
    31681 actactcttc cttttctagt cgacatagta ccttcttcaa gtgcaaccat accaaatcca
    31741 acatgaatta aatcttttgc ccaatcatat cccataagct caattacctt aaaccattgt
    31801 tggaaatgta agttttgttg agaaccaact acatatatac acttgtcaaa atcataagtc
    31861 tcttttctat atattgcagc agctaaatct cttgtcatat ataacgttga accatcattc
    31921 ttagtaataa gtgctggtgg cattttatac tcttctaaat caacaattct tgctccttta
    31981 gactctttta aaagattttt ctcttctaat agttcgataa ctctgttcat tttatcagaa
    32041 taaaaactct ctcctgctaa agaatcaaat tctatatcta aaagtttata cactctgtta
    32101 aactctttta aactctcatt tctaaaccat tgccataatt cttgtgcttc ttcgtcacca
    32161 ttttctaatt tattaaacca tgctcttgct tcatcttcca tttctgggtg ttgttcagct
    32221 tcatcatgga atcttacgta taattttagt aattctggaa ttggattaga ctctactact
    32281 tctttttctc cccacttttt aaaggccact attaacttac caaactgagt tccatagtca
    32341 cctaaatgat ttattcttat agtcttgtat ccttgcgaat catatatctt atataaagca
    32401 ttacctataa ctgtagttct tatgtgccct atatggaaag gttttgctat atttggagaa
    32461 gaatattcaa ctattacagt tttatctttt ccaaaatcac tatgtccgta attttctttt
    32521 tcatctaaaa cttttttaat tacagtttct gctaattgag atttatttac aaagaaattt
    32581 acatatccgc ctaattgtat tacttttgtt atctctccac ttggttctat actttccgcc
    32641 aattcacttg ctatcatgtt tggagctttt ctaaacactt tggctaattt aaagcatgga
    32701 aaagcatagt cccccatatc cttatttggt ggaacttcta taagtgcctc tatctcttct
    32761 ttcgataaat cttctatttt ttctttaagg caatttgata ttgccacctt aaaatcttgc
    32821 attctgttgc tcctctctac attgttcctg gtttatattg tgcttatctc tttatgttaa
    32881 atcaaatttc gtgttttttc aagtatttca ttacagtgta aaacacttta ataattatgt
    32941 tttctgtata tattaaaaat actataattg cagtttttaa tcacactatt attgattttt
    33001 tataagactt ttttctattt tattgtttct tgtactatcc agttgacttt atatactttt
    33061 tttaatcttt tatcaatttt catacatcaa tattaatttt atatacattt attttgctct
    33121 tttatctatt tttatacatc aatattagct ttatatactt tttttactct tttatctatt
    33181 tttatacatc aatattagct ttatatactt tttttactct tttatctatt ttctataaat
    33241 ctttttcact cttaattttt attccttctt cttctaacag ccttgctgtt atacctttcc
    33301 ctggtatctt tttacctgaa aaactaccat catatattag attacatcca caagaggggc
    33361 taccctcttt tagtattgct tcgctacata catataaatt tgctattttt agagtttcat
    33421 tagcccctct tacaaagttt tctgttacat ccttattatc tttagtaatt acttttccat
    33481 caactatctc agcaggtggt cttggtgtag atagaccacc taactgctct gggcatacaa
    33541 gtgtaaattc tttgcctttt aaatattcct tgactttttc attttcatta ttatcgccac
    33601 tatatttaca atttattcct attaaacatg cagataccaa tatcataaaa atgtgctcct
    33661 atacaaattc ttaatatatt cttatacaaa ttactaatgt attcttatac aaattatttt
    33721 aatttaacaa ttgttactcc tgctccacct tctccatatt gaccacctcg ttgtgatttt
    33781 acatgtctat ttcttcttaa aatgtcttgt agccctgctt ttaatactcc tgtgcctatt
    33841 ccatgtatta ctgttacaga ctctaaacct gccacatatg catcatcaag atacttttct
    33901 acttccatta tagcttcttc taggtttaac cctctaaggt caacttcatt ttttacacta
    33961 ccagattttg accttataat gtttctcgtt gatttagtta catttgttga aacatctttg
    34021 cgtgtttttt gtaaagattt aaaaggtaga gtcatcttca ttataccaat ttgcactact
    34081 gcttcttttc tctttttatc tactgaaact acactaccat tttgatttaa agttattacc
    34141 ttaacttctt cacctggctt taagtctttt atctccttat ttgatacctt tggaactatc
    34201 atacttttta ctgttggctg aagacttccc atagagctgg tcaattcttt tctaagttct
    34261 tctatcttac gattcttttc ttttgaagct ctttcttgtt caagatttct aagttctttt
    34321 attataatat ctacttcttc tttagcttgt cttataattg aaaatgcttc tgatttagct
    34381 ttttcaatca tcttatctct ttgactgacc aatttttcaa gtttttcgtc atactctact
    34441 ttcagttttt caatctcttc ttttaatctt tcagcttctt ctctatcttc tactgctttg
    34501 attctattct tttcaacatt ctgtaacaca tcttctaaag ctatgttttc tgtatttata
    34561 tattctttag cacgtgaaat cacataatca cttagcccaa gttttctaga aatctcaaac
    34621 gcatttgatt ttccgggaac acctataagt aacttatatg ttgggcttaa agtttctata
    34681 tcaaattcaa ctgctgcatt ttctacacct ggtttagtaa gagcataatt tttaagctca
    34741 ctatagtgag tggtagctat acatttagct cctacagaat ttatatcttc taatactgct
    34801 attgcaaggg cagctccttc aacagggtct gttcctgctc ctaactcgtc aaatataact
    34861 aaagagtctg ctgttacgtt ttgtaatata gatactatat tcgtcatatg agatgaaaat
    34921 gttgatagac tctgttcaat actctgttca tctcctatat ctgcaaatac attatcatat
    34981 acacacatac tacttccata atcagcaggt atatgaagtc cactttgtgt cataagtgca
    35041 aatagaccta cagtttttat tgtaacagtc ttaccacctg tgtttggacc tgttatcacc
    35101 aatgtatgga agtctcttcc taaataaatt gtatttgcta ctaccttctt tttatccagc
    35161 agtggatgtc ttccattttt tatgtttaaa tatttatcct catttaaagt tggttctatt
    35221 cctctcatct gtatagaaag ttttcccttt gaaaatgcaa agtccaatct tccaagtatc
    35281 tcttgattag agattaaatc ttcgctcact tctccaacca tagctgaaat ctctgataaa
    35341 attctttcta tttcctcttg ctctcctagt ttcaactgcc tcaattcatt gttcatctct
    35401 actatagtca ttggctctat aaaaagtgtc gcaccagaag atgactggtc atgcactata
    35461 ccagctactt gcgacctata ttcagatttt acaggtacta caaatctatc acctcttaga
    35521 gaaattattg catcttgtaa gtacttttga taagtagttg aacttattat tgaatttaat
    35581 tttgacctaa tggattgatt tttttgggct attcttcttc tgatatctct aagtgtactt
    35641 gaagcattgt cagatatttc tacttcacta attatagcat tgtatatctg gtcttctatg
    35701 tccttgtaca catatagaga atttgataga gattgtatta ttggataatt aaaatcttct
    35761 tcatcactac tagataagct atttcttaat cttctagcca ctcttaaggt atctgctatc
    35821 ataatcagac ttcctgggtc aagagaagca cctatataag ctctctttac cttatcttct
    35881 atatcatgaa taccttccaa ccctactgaa cctctcttta taataattga ttgagcttca
    35941 ctagtttctt ctagcataga tttcacttct acaaaatcag tatttggtac taaattttct
    36001 atatatttta gacctaaaga agatgaggct ttcttcttta acaaatctat tattttattg
    36061 tattctagaa ctcttaacga tttctcattc attttctata ttcaccatcc tcgatatttt
    36121 aactttttta tttaatttta aaaatgtatg ttttcaagca tttccaataa aaaatgcaag
    36181 ttttatttct ttttcatttc taaaattatt atattttttc tcttttagat atattttaca
    36241 catattttgt attatactat gtatgtagct atctatatat tatacataaa catgctttat
    36301 ttgtaatata atctgttaaa aaatcttctt taatattttt aaaggagttt attttagctt
    36361 ataaaaaaat agctacttat atagataaat taaggaggaa catgagtcat gggaaacgta
    36421 ttagaaggat taaaaccaga aagtgttttt aaaaactttg agaaaatatc tcaaattcct
    36481 agaggttctg gaaatgaaaa aggcataagt gattttttac ttagctttgg taaaaattta
    36541 ggtcttgaaa ctattcagga tgaaagctta aacataataa taagaaagcc tgctacaaaa
    36601 ggatatgaaa attgtccagg tgtagttctt caaggacata tggatatggt ttgcgaaaaa
    36661 gaaaagaatg ttgaacatga ttttcttaaa gaccctataa aattaagaat tgatggtgac
    36721 atgatatatg ctactggaac tactctagga gctgacaatg gtatagctgt tgctatgggt
    36781 atggctatat tagaagataa tactttagag catcctgcac tagaagtttt agttactgtt
    36841 aatgaagaag atggtatgaa tggtgcagat gcactagacc ctagcttaat taaaggtcaa
    36901 tatatcctaa atatggactc cgaggaggaa ggatacttat tagttagctg tgctggtggt
    36961 aaaacttgtg ttgttagttt acctgttgaa tacaaggaag ttaaaggtga caaacaagga
    37021 ttattagttg aggttactgg actattaggt ggacactctg gtatggaaat tgttttacaa
    37081 agagcaaatg ctaacaaagc aatagctaga gtattaagtg tattgaatgt tgattatgaa
    37141 ttagcttctg ttgatggtgg tactaagcac aatgctatac ctcgtgaagc taaatgtgta
    37201 atagctgtta ataaagctga tgttgaatct gctaaaaaac aaataaatga cattttaact
    37261 gcatttaaac atgagtttac tacttctgac cctggcatga cttactcagt agctgaaact
    37321 tctgtagaca aagttcttac taaagattgt aaagaaaaag ttgttcaaat gtcttgctta
    37381 actcctcatg gagttcaatc agtaagtctt gatatagaag gattagttga aagttctact
    37441 aacttcgcta taattgaaac taaagaatct actattgaat ttttaacttc tgttagaagt
    37501 tctgttatgt ctattagaga tgaaatcgct gatagaataa gacttttggc tcaagcttta
    37561 ggtgctaatt acgaccttat agctcaatac ccagcttggg aatttaaaaa aggctcaaaa
    37621 ttagaaaaaa tatgcagtga aacttatgag aaattaactg gtaaagttcc tactgttatg
    37681 gctctacatg ctggtcttga gtgtggatta ttacttgata aacttcctca tgctgaagct
    37741 atctctatag gaccagatat gtttgatgtt catactccaa atgaacatgt tagtatccct
    37801 tctgtagcta atgtttggga ttatgttatt gaaatattaa aatctatgaa ccaatattaa
    37861 atttaagttt tgattctaaa tagattttaa ttttaagtaa tctatataaa attaactctg
    37921 taaattaata ctactgttag tataatattt actttcaata ttaacttata gaaataaagc
    37981 ctaatgatag ataataacat gtatcattag gctttattgt aatttaaata ttattaaact
    38041 ttattctgtt ttaaaattaa ttcttatact attttaaatc tatttcttcc ttgtctctta
    38101 gaatcataaa gagcattatc tgctatttga tacagttcat catactcttg accacgcttt
    38161 gttataacta cccccatact aatagataaa ctttctccat ttggcaactt aatgagatga
    38221 atatgattgt atacatcatt acatttattg ataagttctt ccttatttat tatatcattt
    38281 atataaacac taaattcatc tccacctaaa cgacctacta tatcatcttt tgcaaaactt
    38341 tcatcaagta cctttgcaac atcaatcagt actttatctc ctaccaggtg accataagta
    38401 tcattaattg acttaaaata atcaatatct ataatcaata aagctccatt tttttcagtt
    38461 gataaattta ataaattttg tgtagttaat tttctaatat atgctgcatt ataaatattt
    38521 gttaaactat ccctttgaac tttatccaac aatctattct tttcctcttt ttcagaatca
    38581 atattagcta ttttaccaat tacatacatt ggtatttttc tatcattata aattattttt
    38641 gaagtaattc taatccaatg aaaagtatca tcaagcatga tagaatacat ttcagtagtc
    38701 ccattttctt gtgattttat tacatcaaaa aactttttat tatattctct tcttatttta
    38761 tctctttcat tttctatatt gccattttca ctttccttat cacttttatc caaaccataa
    38821 taagtctcaa tacccttttc tggcatagaa attatcaatt tatcttgctt ataatcatac
    38881 tcaaaaaagt attcacttgt catttcatac aactgagtat gttttttgag ttccaaagat
    38941 atcttttcgt tcatacttgt acgagtatgt aaaccaaatg ccataaatcc aataatacaa
    39001 attaataaac ttgctacaat aaaaatagtc tcaattggat tctgttgcat aatataaagc
    39061 aacgagaaat cttgtctata tgtagtattt tggtagacaa ttgattgcat tttttctgat
    39121 gaaatactta taatagcttt attaaatata ttcaataatt cttgtttatc ttttttaatt
    39181 atgccaaaac aaatactctt tgcttcataa gtttgaggaa ctaggcgcac atttttatac
    39241 ttaggttgat tcatatagta ctgcatagaa taaccttctc cataagtgta atctgcatag
    39301 cctttattaa cagcttctat acactcttct aaattatcat accaaactat attttcttca
    39361 aactgaccat aataatcaat acctttggct agagctatac gctttccatt tagactattc
    39421 tcatttaatt tattattaat catcatagta tattgagtag aaagaaaagg ccgagacata
    39481 gctatattat gttcacgaga gtactcataa tcataaatca ttcctgctac aatatcaatt
    39541 tcatgttttt tagccatatc ttccaaatct tcttgagatt ttacaggcac aagttcaaat
    39601 tccaaacctg ttttatcaca tatatgattt aacaaatcaa ttgatatacc tcttaattct
    39661 cctgtatctt tatctgcatt ttgaaacggt ggtttgttta aaagtactcc tacttttaat
    39721 ttttctgtat ctttaatgta attagtttct tcatcagata aaaacatttg attattctct
    39781 ggcgtaaagt atttctcata taaagtagtt gaaaaatatg ggtccttctg ttcaatatta
    39841 ttaattgctg aattaagctt ttgagttaag tctgtactgc cttttggagt aatgaaataa
    39901 aatggctttg atgagaattt tgctacagta cgcacacctt ctattgggtt catactaaca
    39961 ttaagaagta catctgcttt accactcttt agtacttcta attgctcgtc acttgtttta
    40021 caatatacca attcaggtga cataagattc attttacaat actcatctaa ttctttaagc
    40081 ctagtatcag aattttctat tacagcaata cgaatttttt tcacatcttg cgaactcatt
    40141 atgttatcaa tatctgtatt ttcatataat gcttgtagta ctgtatttgc tactccataa
    40201 ctgtttcccg cataattata tattttttgc attggctcat tgtataacat acctcccatt
    40261 atatcaattt caccattttc taacatcttc attaattttg taatagattc atttatctct
    40321 ccaggaactt gaacaaattc ataattccat ccagtatact gtgcaatttc tagcaaatat
    40381 tcatatgtat acccaaaata gttaccttgc tcatctattt cagttaaacc tttttgaata
    40441 ggataagcaa cacgaatggt acgattatca ttacctatag cattgatacc aaaagaaaat
    40501 atgcaagaag tgaatataga tgtaattaca ataattaata aaagtaattt ttttaaattc
    40561 actacacaat cctccatata gttaagattt acagtataaa ttactgcata aacttgtaga
    40621 catgaaaaaa tagattagta tataaaagga tatataaatc tacttagaaa tttaaatatc
    40681 cttaatatat acaatatatt taaattataa ctcatttata tatctattac aacaaattta
    40741 ccctgctaaa tacctactct atatcccata attttacaag ttgtaatgaa gtttaagttc
    40801 tatctcattt caaatttagc agtgtttcta tttacagttc ttttatattt cacatctgga
    40861 taacccaata taaatgaagt gataactgct tcgttctcgc tcattcctaa cagttctttt
    40921 atctttggct ctacatttga tgccataaca aaaaatccat tataacaaat tcctaatcct
    40981 tgagaatagg ccataagttc catattagat gcagccaatc ctccatctac ataagcactt
    41041 ccacctagag acatatcacc tacaactatc ataagtagtg gggcatcata aaataaagaa
    41101 tcatttccgt tctctttata tcttttgtac attttcttaa atgtattttt atagatagaa
    41161 cgtacagggt cattgtcatc agtatctaat gccaaattat aaagaccttg tattgccagt
    41221 tctttcactt cttttagctt ttcttttact aatatgtatc ttataggctg tctattacca
    41281 cctgttggtg tgtatctacc agcttccact atgtttttta ttttttcttc ttctacctct
    41341 atatctttat attgtcttat acttcttcta aatttaataa aattcaataa cttttcgggt
    41401 tctaattcaa aactttcacg attgtattct attacttcat ttttatcaaa tccttgcatt
    41461 tctattgcat ctgttgggca aattgccata cagtgaccac agttaataca gagaatattt
    41521 ttaacctttg ctttatcatc cttaactttt attgcattta caagacagtc tgattcacac
    41581 atcccacagc ctacacattt ttctaaattt atgtctatca ttccaaatta ttcctccttt
    41641 taaaaaattt actttagtat aaaacaaata gttgtctaag tcaattttta tttttttaac
    41701 aatatactag ccttaactac actttaatgg agaaaaatat acataaaatt agaacttaaa
    41761 ttagaattta ttcaaaataa aagataaaag aagtaaatgt ctaaaagtga actttttagt
    41821 taatgagaca ttctttattg tatgaaatca agtggatatt tatcatttca acaataaaaa
    41881 aggcttacct ctattttgga tatgtccttg ttaagtagac agactaaaaa aggctatctt
    41941 tatgcactga gatagcccct tttcccaact actattgttt attttaatat tatatcaata
    42001 aatttaattt gctataacct gtaagttaca ttttaatttg atgtaacttt aaattacatt
    42061 tttaagtaat atctttaaat tatattctta aaatatatca ttaaactaca ttttaattaa
    42121 tctaaaaact atatcttaat taatctaaga aacttacatt ataatctttt agtttcccat
    42181 tatcatatac aatctttgca ctaaagatat ttccagtctt agcccaattt aagaacaacc
    42241 tgtcaccttc ccacatattt aaatcgaata ttttatcatt accaacccaa attaaatttc
    42301 cttctttaca atcatttaaa tctttttcag acaattcacc ttcaaactca tctgagaaat
    42361 acaaaaacat ataccaatct tcttcatctt taaattttgg aaaagttatc aaccctttat
    42421 atgacaatga tttaagagta agaccagtct cttcctttac ttccctaatt acacattctt
    42481 ctggagtctc tccttgctca aactttcctc caactcctac atacttacct tcatgtatat
    42541 catctttctt tttatttctg tacaacatca aagtcttgtc atctttttct atataacaaa
    42601 tagttgtaag tttcatgtat ctcgccttac tttctcttta tattctcaat taattatttt
    42661 cagcactttc aagcacatca cttatattct tcattgcatt aagagaaagt tctgcaaatt
    42721 catcaaatgg aatccctata tcttctatac ttctcattgc atctctattt acatttgaag
    42781 caaatctcgt ttctttcatt cttttaataa cagacttagt ctttacacta gtaagttttt
    42841 tatctggata aactaaagct atagcagtta taaacccagt tatagggtct gctgcatata
    42901 ttgctttttc cattaagcta gttcttgtaa ctcccattcc atcatgatga ccaagtattg
    42961 ctctatacat ttcttcgtca ccgaagtttt cttttttaag cagttctact gttgttgtag
    43021 catgtccttc acatatttct cctggctttc ttccaactat atctgaatcc aaatcatgta
    43081 aaagacctgc cactgcccac tcttcttctt tttctggctc tagtcttttg gctaattctc
    43141 tcataacagc ttcaacagca taacaatgtt ttctcattct atcagtttct aaatacttaa
    43201 ataaaatttc taaagcttct tctctcattt tcaccaatcc ccctgttttt tcacatatat
    43261 gatattttta tatattaata cacataaatt caagtcttta tatatattca tgtatatttt
    43321 tcatgttata tctattctac acctttatag tagtgtcctg caactgttcc aatttcatct
    43381 aatttcttat acaaatctgt agcctttttt ccaagtttat aattattact caatacctca
    43441 atgtgagctt caataacctt tttaaccatc tcactatctt caactgtaaa atctatccta
    43501 aatacatcta ttccaatttc ctctaactca tatagattgt ccaacataca agtaaccttc
    43561 gaattgtaaa ttgtacttct acagaatata tcttgtgtta tcctataact tgcatccgta
    43621 aagtctctta aagcataact actttcctta catttagcaa ctctcttgtc ctttttacaa
    43681 tctctaacaa ctactcccat aggacagtat tctgtaacca tcataggagc atatccatat
    43741 attactgctt caatttctac atctgtatat tttaaagttt gtttaatttc atttaagttt
    43801 agttcttgtg acaaacaaac tgtacttgct ccctcttctt tgaagtggtt tatactttca
    43861 ctattgaatg catttaagta gcaatctata tttaagtctt ttttcttaaa gtattccata
    43921 gagccccagt taccaatttg aaccttatta attcctttta tattatccaa tatctcaaat
    43981 tgcttgtact ctttatttct aacaatcctt ggtgctgaat agattaattt tttgtcagtg
    44041 actgctgtat tatagtggtt cttattcaaa gtatctattt tacttgtatt tgacaaagta
    44101 tctatgttat ttgtatttga acccattagt tctttaggaa tacctaaaga cgaagaagta
    44161 tctaaatcca tagaaatact tgaatctata gaaatacttg aatctataga aatacttgaa
    44221 tctatagaaa tacttgaatc tatagaaata cttgaggcca tttgtaaagc ttttgataat
    44281 gtatttatat cttcataata gattaaatct acattataat ttaatactgc ttctaattgc
    44341 tccaagttct taactttaac tctaatctta ggaactgtat caagtttttt ctgtttattt
    44401 actctatttg gttggtattt taatctattc ttcttaaatt ttctatcttt tatctttatt
    44461 ctttcttcat ctaaaagctc aatacaatcc cttctcattt gatttaacac acttataggc
    44521 atacttacac cttcatccag gtcaatttct atctctttta acttatatgg tgtatttcca
    44581 agcttttgaa tttgtgtttc tattttttct ttacttaaag ccacctttat agctttttca
    44641 actattttgt ctccttctac caccgattta tttccacaaa aatcacttat aaaaagctct
    44701 ggctttttac ctagtttaat actcactttt gcacgtatat tagtttttat gaactcttta
    44761 tcttgctgaa atgtagcttg tactctattc attaattcgc tgtcagaagt cttatatata
    44821 agctgatttc tctttgcttc tccaacaaaa tctaattcta tcaaatcccc tttatgtgct
    44881 attgtagtta tctctccatc ctttattatt cttccaatag tacctccacc taagttaatt
    44941 ccatcacctt tcttaagcgt attttctagc attactctaa gtcttttgtt ctttctatta
    45001 taatctaaaa cttttccaat atatagccct tgattattag gtctattaga gttcattata
    45061 tccttaccaa cttcacctaa tatatatcct tttgtaaact ttctattaaa tatggtatac
    45121 aaattattta ttatttcctc agaaatactt atttttttct tatctacaaa ttcattaatc
    45181 gcttctctat atcctgaaac cacagtagct acatactctg gttttttcat tctcccttca
    45241 atttttaaag ataatacacc tgtttcaatt attttatcca aatcttctat agtacttaaa
    45301 tctctaggac tcaacaaata atctccatta ctttttacta cttctcctgt atttatatct
    45361 atcaactcat acttttgtct acatggctgt gcacacctac ctctatttcc agacctattg
    45421 cctatcatac tactcataag gcattgaccc gagtaacata cacataaagc tccatgtaca
    45481 aaaacttcta tatctacagt tgtattttca caaatccatt ttatctcatc aacatttaat
    45541 tctcttgcta aaacaactct atcaaaccct acactctgaa ggtattttac atcttctaag
    45601 gaatgtgcta ccatttgagt gcttgcatgt agttcaaagt caggtaatag tttttttata
    45661 agtcttgcca ttcctatatc ctgtactatt aaagcatcaa tatctatatc gtataaaaat
    45721 tttacatact ctacaaaatc ttctatttca tactgtttta tcaatgtatt tacagtaaca
    45781 aagacttgca ctccccttat atgagagtat ttaacagcct caatcagctc atttctatca
    45841 aagttatttg cagatgctct agcactaaag tctttaccac ctaaatacac agcatttgct
    45901 ccgttttgaa ctgctgcttt taatgaatca aaagagccta ctggtgcaag taattctatt
    45961 tttttcatat ttttctcctc tatttatact tgttaggtta atataattat atctataact
    46021 taaatatttg tattaaattt atatatttct tactatattt taaattcttc aattaattta
    46081 tatattagtt at
//
LOCUS       DAMKPG010000021        45945 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMKPG010000021 DAMKPG010000000
VERSION     DAMKPG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.023"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(72..275)
                     /locus_tag="PC621_001270"
                     /pseudo
     CDS             complement(72..275)
                     /locus_tag="PC621_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(275..604)
                     /locus_tag="PC621_001271"
     CDS             complement(275..604)
                     /locus_tag="PC621_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF4108438.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            934..1860
                     /gene="fba"
                     /locus_tag="PC621_001272"
     CDS             934..1860
                     /gene="fba"
                     /locus_tag="PC621_001272"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF4108439.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(1995..3626)
                     /locus_tag="PC621_001273"
     CDS             complement(1995..3626)
                     /locus_tag="PC621_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108440.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4109..5146)
                     /locus_tag="PC621_001274"
     CDS             complement(4109..5146)
                     /locus_tag="PC621_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF4108441.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5165..5950)
                     /locus_tag="PC621_001275"
     CDS             complement(5165..5950)
                     /locus_tag="PC621_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF4108442.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(5983..7116)
                     /locus_tag="PC621_001276"
     CDS             complement(5983..7116)
                     /locus_tag="PC621_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF4108443.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC621_001277"
     CDS             complement(7164..8291)
                     /gene="hadC"
                     /locus_tag="PC621_001277"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF4108444.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC621_001278"
     CDS             complement(8291..9517)
                     /gene="hadB"
                     /locus_tag="PC621_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF4108445.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC621_001279"
     CDS             complement(9532..10332)
                     /gene="hadI"
                     /locus_tag="PC621_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF4108446.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC621_001280"
     CDS             complement(10371..11570)
                     /gene="hadA"
                     /locus_tag="PC621_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF4108447.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12247..13245
                     /locus_tag="PC621_001281"
     CDS             12247..13245
                     /locus_tag="PC621_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF4108448.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13607..14134
                     /locus_tag="PC621_001282"
     CDS             13607..14134
                     /locus_tag="PC621_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF4108449.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14161..14757
                     /locus_tag="PC621_001283"
     CDS             14161..14757
                     /locus_tag="PC621_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF4108450.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC621_001284"
     CDS             complement(14822..16405)
                     /gene="asnB"
                     /locus_tag="PC621_001284"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF4108451.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(16971..17807)
                     /locus_tag="PC621_001285"
     CDS             complement(16971..17807)
                     /locus_tag="PC621_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4108452.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(17831..19297)
                     /locus_tag="PC621_001286"
     CDS             complement(17831..19297)
                     /locus_tag="PC621_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF4108453.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(19300..21162)
                     /locus_tag="PC621_001287"
     CDS             complement(19300..21162)
                     /locus_tag="PC621_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4108454.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(21409..22272)
                     /locus_tag="PC621_001288"
     CDS             complement(21409..22272)
                     /locus_tag="PC621_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF4108455.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(22327..23259)
                     /locus_tag="PC621_001289"
     CDS             complement(22327..23259)
                     /locus_tag="PC621_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF4108456.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(23345..23896)
                     /locus_tag="PC621_001290"
     CDS             complement(23345..23896)
                     /locus_tag="PC621_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108457.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(24125..24616)
                     /locus_tag="PC621_001291"
     CDS             complement(24125..24616)
                     /locus_tag="PC621_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108458.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(24644..25204)
                     /locus_tag="PC621_001292"
     CDS             complement(24644..25204)
                     /locus_tag="PC621_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF4108459.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(25356..26513)
                     /locus_tag="PC621_001293"
     CDS             complement(25356..26513)
                     /locus_tag="PC621_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF4108460.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(26550..27491)
                     /locus_tag="PC621_001294"
     CDS             complement(26550..27491)
                     /locus_tag="PC621_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108461.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(27578..28270)
                     /locus_tag="PC621_001295"
     CDS             complement(27578..28270)
                     /locus_tag="PC621_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4108462.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(28380..29192)
                     /locus_tag="PC621_001296"
     CDS             complement(28380..29192)
                     /locus_tag="PC621_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF4108463.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(29243..30154)
                     /locus_tag="PC621_001297"
     CDS             complement(29243..30154)
                     /locus_tag="PC621_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4108464.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(30213..30944)
                     /locus_tag="PC621_001298"
     CDS             complement(30213..30944)
                     /locus_tag="PC621_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF4108465.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            31033..31284
                     /locus_tag="PC621_001299"
     CDS             31033..31284
                     /locus_tag="PC621_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108466.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            31517..31936
                     /locus_tag="PC621_001300"
     CDS             31517..31936
                     /locus_tag="PC621_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108467.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            31961..37018
                     /locus_tag="PC621_001301"
     CDS             31961..37018
                     /locus_tag="PC621_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF4108468.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(37178..37915)
                     /locus_tag="PC621_001302"
     CDS             complement(37178..37915)
                     /locus_tag="PC621_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108469.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(37934..38869)
                     /locus_tag="PC621_001303"
     CDS             complement(37934..38869)
                     /locus_tag="PC621_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF4108470.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            39077..41662
                     /locus_tag="PC621_001304"
     CDS             39077..41662
                     /locus_tag="PC621_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4108471.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            41674..42345
                     /locus_tag="PC621_001305"
     CDS             41674..42345
                     /locus_tag="PC621_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108472.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            42372..42713
                     /locus_tag="PC621_001306"
     CDS             42372..42713
                     /locus_tag="PC621_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108473.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(43073..45715)
                     /gene="adhE"
                     /locus_tag="PC621_001307"
                     /gene_synonym="adhC"
     CDS             complement(43073..45715)
                     /gene="adhE"
                     /locus_tag="PC621_001307"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF4108474.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        15411 a         7651 c         5618 g        17265 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac gagtgtacgt
       61 agctattttt taatcaaata tatatattct ctatatataa cttcttctcc attaaataat
      121 atctcatcat tttcagtgtc tacttgagtt cctggatttg aaacttcact tccatttact
      181 actacagacc cttgtttaca atacttcttt atttcacttc tacttccata acctaaattt
      241 gaaagaattt tatctatacg ttgcttttta gccattaaaa atgctctcca ttctcataat
      301 atttttcttt ttcaaatact gtaaatgcta aatccatact ttctacatct aaattatttc
      361 ttatactatc agttattatt cttgctacta tatcttgtac ttcttgtcct ctatcaaacc
      421 aagctacttc aacaaaagga tatacatctg ctattttacc atctcttatt gcaactgact
      481 ttatacattc tatttcaaag tagtctcttg gacattttac agcttcaact aaatcattta
      541 tcattttttt acttatttta catatatcat tttcattaat tcctcttatt tttatctgtg
      601 gcatagttta tctcctttat tttaaatttt tagtgaatta tcatccactt aattcccttc
      661 gataacgttt gaagtggtaa cttcttggtc aagacaacta ttgttatcag tatctccaaa
      721 ctaataaggt agttcctacc cttatagtta gcattatata gctaattttc ctaaattatt
      781 tatattcttt attattttat ttaatcctat tgtcttctat ttaacattat acttgtaatt
      841 tatagttatt acaatttact atatactttt tttgatttaa cgttataata taagtgatgg
      901 gtaaatttta aaagttaata ggaggagttt aaaatggcat taattactac aaaagaaatg
      961 tttaaaaaag cttacgaagg tggatttgct ataggtgcat ttaacataag tgacttagaa
     1021 caattacaag gtgttttaaa agctgctaaa gctaaaaatt cttatgttat gatacaagct
     1081 tctatgtcag ctgttaaata tgctggacct catacattag ttgaaatggt taaggctgct
     1141 tctgatgaaa taggtgtaga tgtagctctt cacttagacc atggtccaaa tatggatgct
     1201 attaaaactt gtattgatgc tggtttctct tcagttatga tagatggttc tcattttgac
     1261 tttgaagaaa atgttagaat aactaaagaa gctgttgaat atgcacactc taaaggtgtt
     1321 gttgttgaag cagaacttgg tgttctagct ggaacagaag atgatgttac ttctgatgtt
     1381 cacaagtata ctcaaccagc tgaggcagtt gagttcgttg aaagaacagg tgttgattca
     1441 ttagcaatag ctattggaac ttctcatggt gctttcaaat tcaaaggaga agctaaatta
     1501 agatttgata tactagaaga aatccaaagc aaattaccag gtttcccaat agttcttcat
     1561 ggtgcatctg ctgttgacca aaacgctgtt gctacatgta atgaatttgg tggaaatata
     1621 gctggtgcta aaggtgtacc tgtagatatg ttaagaaaag catcttctat ggctgtttgt
     1681 aaaataaaca tggatactga cttaagacta gctatgactg ctgctatcag aaaattctta
     1741 gctgaaaatc caaaagaatt tgacccaaga aaatacattg gtgctggtag agatgctata
     1801 caagctgtag ttgaaagcaa gatagatgat gttttaggtt ctgctaactc tataaactaa
     1861 tataactttt aaatatttat aaaagaaaag agatgactta aagtaattaa atttattttt
     1921 aagccatctc ttttaagttg tatcaattat gtatcttata atattttaat tatattgtct
     1981 ttttaaactg acagctaaac gtgaatatgt ccactcttta tctttctatg gatggtagaa
     2041 ggatatatac ctataacttc agcagccttt gttattgaac catacttatt tatagcctta
     2101 cttatcatta tttggtccac tattttatat gcatcattaa gattcataat tccatttacc
     2161 actattggtg ataaatcatc tgtttcatta tcaatcatat ctaaatttat cagcatgtta
     2221 aattcatctt ctccaattac atttttagct gataatacta caaatctttc aatgatgttt
     2281 tttaattctc ttatgtttcc tggccaagaa tattcttcta ataactcaat aactttaggt
     2341 gaaacttcta cttctctatt gtacttttca ttgtaaagtt ttaagaaata atttactaat
     2401 ggtactatat cctcttttct ttctctaaga ggaggtattt ttactggaat aacacttaat
     2461 ctatagtaca aatcttgtct aaatttaagg ttatttctaa ggtcttcatg agaaatatta
     2521 gtagcactta tatatctaac atctattttt ataggcttac taccacctat tctagtaata
     2581 gtattttctt gtataaccct aagtaatttt ttttgcatct gcattggtaa ctcacctatc
     2641 tcatccaaga aaatagttcc accattcgcc tcttcaaaaa gtcccttttt accttttttc
     2701 ttagctccag taaaagaacc ttcttcatat ccaaaaaact ctgactctaa taattcattt
     2761 ggtatagctc cacagtttat agctataaaa ggtttgtctc tccttgaact atacttatat
     2821 attgttcttg ctattatttc ttttcctaca cctgattctc catttataaa tatagaagaa
     2881 tctgaggctg cagctctctt aacaactttt tttatattgt ccataatctt actttgactt
     2941 atatactctg tgtcttctga tatatcacta tattttatct gagattcaag cttaataacc
     3001 cttgaaatat ctctccctgt tagtactcct cctgtaagct ttcccttaga atcccatctt
     3061 ggaactgccg tatatgcaat aagcttccca tctgggtaaa cgatatttct ataaatcttt
     3121 ttcttcactt taagcatgtt taatattatt ggttcttcaa ctaatccttg tctcacaagg
     3181 tctgttatat ttctaccaag tacctcagtt cttttaaaag gtaatatctc atcactgaca
     3241 gtgttcatct tttcaattac cccatctttg ttaaacacaa caacaacttc atctatatgc
     3301 tctattatat cttcaattac tttatcagat attagagact tctcaaataa ataaactata
     3361 ttattttcat tattattatc tttcatattg tatatatgtg cagtcatcct taactctctg
     3421 aaagctattt tctttttact atctgtcagt atttctgttt tgtcaaaatt tgaatctaat
     3481 tcatttatat ttctaggctt ttttatatct agaaaactaa aaatactatc tattagatga
     3541 ttacagtata ttatttcgcc tgattcttta cataaaatgg ctggattttc tacatgttct
     3601 ataagcccta ttaaattatt atccattaat attcgcacct ttcattaagt aatttattga
     3661 actacataca aaatttattg tttgcttaca ataaatacca tcagcacata taatcaactc
     3721 cttataatat tcgtcttttc aagtagttta tttcattaaa acgtaactaa gatataattt
     3781 taatgattat agtttgttaa aaatttcatt ttgcttttaa ttgtatttta tcacatattt
     3841 agtaattatt tgtctattta atacaattta attaaattta agatttattt aaaaaaacaa
     3901 agataatcct tacagaatat aactcacaat ttgtaagcaa tatacatata aggattatcc
     3961 agattatctt gtattataaa attttattca aacagacttt ttaataactg acaaatacac
     4021 actagcaata acttatcaaa gggttaataa ttatgttact aactaataag acttattcaa
     4081 tcagtaatat ttatatagtt tgtatctatt aagcttttat agcttctaat aaatcatcaa
     4141 cgctatctaa tttttctata agcattggaa ctatttcatg taagtctcca actactccat
     4201 agtcagcgat ttctaaaatt ggagctgctg ggtttttgtt tatagcaatt atgaaatctg
     4261 atgattgcat acctgctaaa tgttgtattg ctcctgatat accacaagct atatataggt
     4321 ttggtttaac agtagttcct gtttgaccta cttggtgaga atggtctatc catccagcat
     4381 caacagccgc acgagaagaa ccaactactc cacctaattt gtcagctaat ttcttaagca
     4441 tttcaaatcc ttctggtcca cctaatccta aaccacctga tactataaca tttgcatctg
     4501 taagagatac taaatctttc ttagttttaa ctgtttctaa aacagtagtt cttatatcat
     4561 cttgagttat tttgtagtct aatgctataa cttcaccagt tcttgtttcg tctttttcag
     4621 ctttatccat aacaccaggt ctaactgtag acatttgagg tctatggttt ggacaaatga
     4681 ttgtagccat tatgtttcca ccaaatgctg gacgagtttg ttttattttc ttatcttctg
     4741 ggtctatttc taacttagta cagtcagctg ttaatccagt tccaactctt gaagctattc
     4801 taggtgctaa gtctctacct atatgagttg ctccgaaaag cattatttct ggttttattt
     4861 catctattgc atcttttatt acttttgtat atgcatcagt tgtatatttt tctaaaagag
     4921 catcatctgc aacataaact ttgtcagctc caaaagtgat taattcttta gctaatccat
     4981 caacatcttt tcctaataat attgcacaaa gttctgcatc tacttcttta gctaattttc
     5041 ttccttctcc taataattct ataactactg gagctatttt tccttctctt tgttctgcaa
     5101 atatccatac gtttttataa gaacttaaat cttttatatc attcatagtt tgtactcctc
     5161 caacttatat aatgtgcttt tgttttagac cgattattat tttactagct atttcatcag
     5221 ctttaccttc taagatttct ccttttcctc ttggttcagg agtaaatgat ctaaatactt
     5281 tagttggtga tgcttttaaa ccaacttctt ctttatttac atctaaatca tcaattgtcc
     5341 aaacttttac atcatgttta tatgctttta ctattttatc aacagacatg tatctaggtg
     5401 tatttaactc ttttactgct gttaaaagta caggtgtact tacttttatt aattcgtatc
     5461 catcttctaa ttgtctttga actattatat cttttccttc tattttgaaa tcttgtacat
     5521 atgtaacttg aggaatgtct aatttctctg caatttgtgg tccaacttgt gcagtatctc
     5581 catctattgc ttgtcttcct gcaaatatta tgtcatagtc acctacttta gatattcccg
     5641 ctgcaatagt atttgatgta gcccaagtat cagctcctcc aaatgctcta tcacttaata
     5701 atattgcatc atcagcaccc atagctaaac actctcttag cataaagtct gcttgaggag
     5761 gtcccattgt tataactgtt atagttacat catcatgctc atctttaatt tttaaagctt
     5821 cctctaatgc atttgcatca tctgggttta atatacttgg aactccatct cttataagag
     5881 tacctgtttc tttatttatt cttacttcgt tagtatctgg aacttgtttt acgcaaacta
     5941 atattttcaa agtctatacc tccaatttca ttagattatt tattatttta atacgtttga
     6001 tgaaattacc attttttgaa cttctgatgt tccttcgtat attgatacga ttctagcatc
     6061 tctgtacatt ctttcgattt cataatcttt tataaatcca tatccaccgt gaagttgtaa
     6121 tgctttataa gcaacttcat ttgcaatttc tgatgcatag tatttagcca tagcagattc
     6181 ttgagcaact ctcattcctg catctctctt ttgtgctgca tcataaacta atcctctagc
     6241 tgcacaaact tttgtttcca tgtcagctat agtaaattga gtattttgga atttagctat
     6301 aggcttacca aattgaactc tttgttttgt atatttaaca gcttcatcta aagcaccttg
     6361 agctattcct agagctaagg cagcaacacc tattctacca acttctagag tacccattgc
     6421 tatcttaaat ccttgacctt cttttccaag taagttttct tttggaactt taacgttttc
     6481 aaatattaag tcagaagttt cagttcctct tatacccatc ttatcttcat gagctcctgt
     6541 tgagaaacca tcccatttac tttctactat aaacgctgat gttcctctaa gtcctttact
     6601 tctatctgta actgctatta ctatagctac atcacagaat ggtccattag ttatgaaagt
     6661 ttttctacca tttaatatat agtggtctcc aactaattct gctgttgttt gttgtccacc
     6721 tgcatcagaa ccagctcctg gctcagttaa cccaaatgct cctattaatt cacctgttgc
     6781 tatacctttt aagtattttt gtttttgctc ttcagtacca ttgtaaagta ttggtaatga
     6841 agcaagagaa gaaccagctg taacaaaagt agctgttgaa gcacatcttc tagctatttc
     6901 ttccattact atagcgtaag aaacatatcc tgctccccca ccaccatatt cttctgggat
     6961 attactgctt ataaattttg catcagctaa ttttttgatt aattccttag gcattacacc
     7021 tgttttatcc atttctgctg gtaaagtatc taattcttta ctaacaaaat ctcttactgc
     7081 ttttcttaaa agttcttgtt ctttgttata taacatagct tcctccaatt ttcatatttt
     7141 atagtaaatt tattttaatg aacttacgct aaactcatca tctcagcaaa agtttgaata
     7201 cgagttctgg cttgttcatt attctgagtt tgttggtcga tttcaacata taaagtaggt
     7261 attccactat cttctatatc ttttctaact aaaggataat cgtattcttc tgggtcacag
     7321 aatttcatca tacagaagat aacaccatct atatctttct ttttaacttc atctactata
     7381 agtgacccac gttttttctt agggtcataa gctaatgaac atccttctat gtttgaccat
     7441 tgtcttgcta atctctctaa cgcatcatca cctgctggta catctgttct gaattgtctt
     7501 gtttcttgtg ctaagtcgtc agctacaact gatatattgt tgtcttctaa aatgtctaat
     7561 atatctttag aatcagctaa tatacctgtt aataaaactt tttttccaga acagacttct
     7621 tctggcatag catttaattt tgctattaaa tctttcacta gctcagtatg ttcttctttt
     7681 ctcataaaga acccactttt aattactaag cttcttattg atggttttat agtattagaa
     7741 tgtttataag ctacttcaac aaagtctctc atagtttttc tatgttcatt gtaaacttct
     7801 atactttcat gaatttttgc ttcttctatt tcatatccac aaatttcttc aagttctctt
     7861 tttacacctt tgtactcact gattaagtat tttacaccag cttcaagttt tctattttgt
     7921 gggtgtacta atgatatata ttttatatga ggtactgctg atttccagtt ttgtcctaaa
     7981 caaattagtg tatcacacat acctggtata ataactccag ataattcatc ataagcacct
     8041 tttaatccat attctaaaca tgattgcatt attgaacatg caaatgcagg gaaatattgt
     8101 ttagctaaat ctaattctgt ttgtcctccc catataccaa ctggaagcat tccagctgca
     8161 tgtataattt cttctgggca ataaactggg aaacaaccta tagctttttt accagtttca
     8221 tttttatatt tttttacagc cgcatttgga ttttcaacta cttctttcat tttagataaa
     8281 atagcttcca ttatatctca cctctattaa gtttttttct ttcttccatt acttcaacta
     8341 aaccttgaat tcttgtttca aattgagcat tagtgaaagc tctagggtct gcttggtcac
     8401 catcaaatcc agcatatggt aatccagttt cttcagctgc tcttctttgc atttcatatt
     8461 gtattaaact cataagttta cagcttctgt tcatatgata gaatgctcca tcacatttac
     8521 cctctactaa agaatcaact ctatattttg tcatacggtc taggtttaca ttgttaaaca
     8581 tagtactata tgctactgcc attccatcta aatcattaac ttcatattgt aatgcccaag
     8641 catgtgggta aacactacct gtcatgttaa ctccaaattt agctaatgtt ttcatcttgt
     8701 atcctatata tggccaacaa ggtatacctt ccatcattat tctgtatttt tcttcccctc
     8761 tgaaagaaga tttaccagtt ttcatgttgt cttctaattc ttctataagt aacttaaatg
     8821 cttctgtagt ttctttttta cctctagcac aaactattac agccatgtaa gtaaataagt
     8881 caaatccatt cattggagaa ggagaagaat ctgctggtaa actcatagaa tacttccata
     8941 gtcttccgtt ttcagctgat attttcatta cttcttcaaa cttcttaggg tcaaatttct
     9001 ttcctgatat aatttctagt tgttttatag cttcttcaaa ttgagcttta atataatcta
     9061 ttcttgattg agtaacttcg tcttcattat tgaaagttgt atcaatcatt attaaaggta
     9121 tatctaattc tcttgaaata ttttcatacc attttataac ttgattacat atattgttac
     9181 agcaaagtag gaaatctgga gctggcatat ccaaagcttc acatccacca ttttctaaaa
     9241 gaccaaaatt tgttcttgca tatgcacata ggtcaataga atatccttta gattcagcta
     9301 tttcacataa ttctaaagaa ccttttttag ctgctactcc agctgcttgg ttttctggat
     9361 ataatacgtt taagtcaaat acttctgcta actcttgagg aaatactgat gttgaccaac
     9421 ctacaggtct tccttcttct ttagctttaa atgcatttgc atattgttca gctaataaat
     9481 cattaattac tactctagct tctttttttt cagacatttt catccctctt tttatatatt
     9541 tttcacttct ttttgtgatt cttttgcttc atcaaaagca tataacgctg ctcctaaagc
     9601 accagttaat tgaggtatat caggtacaat aatttctgtg ttgatttctc ttgccatagc
     9661 tcttacaata ccactatttc tagcaactcc accaaccata actacatttc tttgtactcc
     9721 tattcttttt actaggctag aaactctctt tgctactgaa gtatgaatac ctgccactat
     9781 atcttcaatt tttgcatttt cagataaatg tgatataacc tcagactctg caaatactgt
     9841 acatgtactg cttattgata cttcattttg agaattcata gatatacttc cgagttcaga
     9901 tacatcaacc tctattattt ttgccattac atctaaaaat cttcctgtac ctgcagcaca
     9961 tttgtcattc ataagaaagt ttaatagtct tccattatta tctaatttta atacctttgc
    10021 atcttgacca cctatgtcaa taatggttct tgtctctgga attatgaaat ttacccctct
    10081 agcatgacag cttaattcac ttatttgctt atcagcatct gaatagttca ttcttccata
    10141 tcctgtaact acaacttttt taatatcttc tcttgcaaga cctgtcttgc catataattt
    10201 ttctaaaact cttgatggtc cagtagtccc agtaccagag gatattgttt cagaagctac
    10261 aatatcttcc ccattcttta agattactcc ctttgatgca gttgaaccta tatctaatcc
    10321 cattgtgtac atctttagca ttctcctttt aaaaaatcta gaattactaa ttaatatctt
    10381 acaactttac tatctttaaa gttatttatt ttctcttcat cgtagcctaa agattttaat
    10441 acttctacag tatgttgacc tacttttggt gctggtgtat attctttaat tccttcattt
    10501 ctaaacataa ctggagtatt aactaagaca cctgtatttc cgctatcgta tgttttctta
    10561 aataagaagt catttgccca agcttgttcg tcatctaata aatcttcaca gctttgaatt
    10621 ttttcaaatg gtaagtctgc ttcttctaat aaagctgacc actcgtctaa tgttttttct
    10681 aacatagctt ctccaactat cttaactaaa tcttcaacat gattaaccat tgaatctatg
    10741 ttattatatc tatcatcttc taatatatat tctctattta taaccttaca gaatttgcct
    10801 aaccacttgt tgtattgtat taaagctaat tgaatccatc ttccatcttt acatttatat
    10861 gtagtcatta atgggctgtt tggattttct cttgataaag gcatttcatt tccgtattgt
    10921 gctgttgtta tcattgttcc cattccatat atagctgtat ggaaaagact tactgttact
    10981 ctctcacctt taccagtttg agctttttta tgtaatgctg ctaaacttcc tgctgctagt
    11041 gctagacctg catagtggtc accaaatcct gctgctgtat ttgctggaga tgttcctttt
    11101 tccataacag attggctaac tcctcctctt gcgaagtatg cagtatagtc aaatcctggt
    11161 ttatctttta aaggtccttt ttcaccatat cctaatattt gagagaatat taatcctgga
    11221 tacttatctt ttatttggtc ataagctata cccatttttt ctaatgcttg aactctaaca
    11281 ttagttacaa atatgtctgc ttctgataat aatttatgta atatttctac tccttctttt
    11341 gattttacat taatacttac acccttttta tttccatttt ctaattcaaa catagggttt
    11401 tcatcatctg atgctggaga tttaaatgtt ccacccataa ctcttattcc atcaccttct
    11461 ataggttcaa tcttaataac ctctgcaccc cagtcaccta acatttttgc acaacatggt
    11521 gctgcgatga aacttgaaag ttctactact ttaactcctt ctaaaagcat aacgttttcc
    11581 tccttaatag tatcaattat aaaatttcga ttaaatcact cataaaatac ttttttctga
    11641 tattaactgt ctgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa
    11701 attatttttt attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact
    11761 ttatatttaa tcaaaatatc gcttgatttg aatgtttttt aacaatatgc aattgaataa
    11821 tctttttcat atcgtggcat cttgccacta tccattgcat tttgctattc tttctatgct
    11881 tataaaagtt ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt
    11941 cattgctaaa ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa
    12001 tttcaaactt agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata
    12061 aattttagag taattattac attaaataaa aaattactaa atatcaaaat aacacaggga
    12121 aaacaatttc aaaactaaat ttttaacctt aaaaattttg tataaaaaat ataaccttat
    12181 atgctacata ttatttctaa atgaaaaatt accataataa tttaataatt acaggagggt
    12241 attgatatga aaatactagt atttggagca cgcgattatg aagaaccagt aataaaaaaa
    12301 tggtctgaag aacataagga tgttcaagtg gatatttatc ctgaaaacat gactgaagaa
    12361 aatgtagtta aagctaaagg gtatgatggt atatctatac aacaaactaa ctatatagat
    12421 aatccttata tttatgaaac tttaaaagat gctggggtta aagttatagc ttcaagaact
    12481 gcaggggttg acatgataca ttttgattta gttaatgaaa atggacttat cgttacaaac
    12541 gttccttctt attcacctaa tgcaatagct gaattagctg ttactcaagc tatgaacctt
    12601 ttaagaaaga ctcctctagt aaagaaaaaa gtctgtgaag gtgattaccg ttggatagct
    12661 gaacttcttg gaacagaagt tagatctatt acagttggtg ttataggtac aggaaaaata
    12721 ggtgctactt ctgcaaaatt attcaaaggc ctaggagcta atgtaattgc atttgaccaa
    12781 tatccaaata gtgatttaaa tgatatatta acttacaaag attctttaga agaccttcta
    12841 aaagaagctg accttataac attacatact cctttacttg aaggaacaaa acatatgata
    12901 aataaagata ctctagctat aatgaaggat ggagcttaca tagtaaatac tggccgtggt
    12961 ggtttaatta atacagggga tttaatagaa gcactagagt caggaaaaat tagagctgct
    13021 gcccttgata catttgaaac tgaaggattg ttcttaaaca aaaaaatgaa tcctggagaa
    13081 ttaactgacc cagaaataaa taaacttctt tctatggaac aagttatatt cactcatcac
    13141 cttggtttct tcactagtac agcgattgaa aatatagttt attctagttt aagtagtgct
    13201 gtagaagtta taaaaacagg aactgctact aatagagtaa attagtattt tataaatata
    13261 ttttttcatt aattagctat tatctaacaa aatagccttt aatttcccaa atataagaga
    13321 gtcagctcag tatatattga gtagactctc ttatattatt ttttatatta aacttgtact
    13381 cctcttattt ataccaaatt aaatttatat taacttactc taactactcc tactctatac
    13441 attttaccta tttttacttt tctaattata tacatttgaa tgggtacaat ctgtatttta
    13501 caaaaaaaac acctaacaaa ctatgaaaat tgtatgatta atattaatat atttataaga
    13561 aaacataaca attatgttag ctctggattt ttagggaggg agtatattgg aaaaaatgaa
    13621 taaagttact tctaaaaaaa ctacctatat tgttacttca gcattatttg catccataat
    13681 atgcctgaca atagcatata tattacatat tccagttggt ggaaacaatg gatatgttca
    13741 tataggagat gcttttattt atctagctgc aactattttg ccaacaaact atgctatagc
    13801 tgcttctgca attggtgctg gtctagctga tttatctaca ggtgctgcta tctgggtaat
    13861 accaactata atcataaaac caatacttgt attatttttc acttctaaaa gtgacaagat
    13921 aataaataag agaaatatag ttgcttcggt tgtagcaggt atagtaggat tagttttata
    13981 catgtttgct gaaggtataa ttattggaag ttttacaagt gcatttgtaa tgagcttact
    14041 tggcttatta cagccaatag gaagttttat tgtttttata atcttaggta tggcacttga
    14101 taaacttgat tttaaaaaac gttattttaa ttaagaattt aaatttatgg agggaaaatt
    14161 atgaatatct tatatacaat tgaaaatagt atctatgtaa atataactaa tacatgtcca
    14221 tgtagttgtg ttttttgtat aagaaacgaa aaagatgaag ttgcaaatag tggtagttta
    14281 tggttggagc atgagccaag tgtagatgaa gtaaaagaag ccttcaataa atacaattta
    14341 gacgactatg atgaaatcgt attttgtgga tatggcgaac cattaatgag aataaatgaa
    14401 ttgatagagg tagcaaagtt tataaaagaa aaaagtagca taaaaataag aataaataca
    14461 aatggtctaa gtgatttaat acataacaaa aaaacagcta ttctgttaaa agatgttata
    14521 gatacagtat caataagtct taatgcccca aataaagaag cttataatag agttactcaa
    14581 ccaaagtttg gagaaaaatc atttgactat atgttaggtt ttgcaagaga ttgcaaaaaa
    14641 tatataaaag aagtagcttt ttcagtagta gatgaaatat ctccagaaga aatagaagaa
    14701 tctaaacaac tggcaaaaaa acttgatata ccattaagag ttagacataa gaattagcaa
    14761 gtaaaattat aaaacattaa gaatcacctg aatggtcttt caggtgattc ttcctcttgt
    14821 attaacattt tacaaaatag atttcatacc acactaggaa tgtgtaaatc atccaaatct
    14881 ttctccaatt atcagagttt ccactaatgt agtcattaaa aatctcatta atctcatcta
    14941 tgttaaagaa tttatctgct atctcactat tgaatttatc tcggatatcc ttattatatt
    15001 ggtcatcagc catccatata cgaattggca caataaatcc taatttattt ctaaaagcaa
    15061 tctcttctgg taatgttttt gatgcagctg tacgaaaggc aattttattc tcttttcctt
    15121 tcactttata ttctgatggt attctggatg caatatcaaa tattcttgta tctgtaagag
    15181 gcattctgac ttccaatcca actgcagtac tcattttatc tatattaaaa taaatatctc
    15241 cttccaacca tatttgaata tctacatttg acatgttagt aagtgtatct agttcttctg
    15301 tttcttcata aattcctttt accacttcaa taggaagcac atcaggattg tatttcttaa
    15361 gaatttttct tttctcaccc tcattcataa tgtttgtatt tccaatataa aatgtcttca
    15421 aattctctcc ataacactct gccttatggt atatattata tccaccaaaa aactcatctg
    15481 ctccttctcc agagtaacag atatttgtgt tttcagctgc tgccttgcat ccaatagcaa
    15541 aaacgatagc agatgcatct ccagttggtt gctccatatg gtacattaca taaggtactg
    15601 acgcaaaata ctcttcaggt gtaattatat atctcgaatt tttacaccca agaatattag
    15661 ctgtctgttg tgctaatttt gattcatcaa aacgctcttc ctcataacca catgaacctg
    15721 ttttttctat atctgacatt gccaatatat aagatgaatc aacacctcct gataagaata
    15781 actctgcagt ttcattctct gattttacct ctggcataat tttctgaagt gttgtatgaa
    15841 tttcatccgt ccattcctca atagacttgc tgtggtcagg atgaaattca ggtttccaat
    15901 aacgaccaat acatactgtt ttattctgat agacaaggta atgtcctggc ataagcttct
    15961 ttactccacg gaagaatgta ttttcacctg ctacatatgt gaaagacata taaatctgta
    16021 acatgtcttc atttaactct ttcacaaatc ctggttgctc cataatctta cgaatagatg
    16081 aaccatagag aagcttccca ttttcagtct cataataata gaaaggtttt actccaaact
    16141 ggtctcttag gcagaagagt ttcttttcca cagtatccca taatgcaaag gcaaacattc
    16201 catgcatgtg atttgccata tcatatcccc atgtcttata tgcttccact aaaattgctc
    16261 gttcacgctc atctctacat aatgaagaat cgattccaag ttcttcacag agaactttcc
    16321 agtttcgaat atgtccattg tattctagaa tttttatcat atatgtgtct tttcgtgtta
    16381 aatacttttc ttctaaattt atcatatgtg tatcctttcg tattaaaaca tttaacttct
    16441 aaatttatta aatgtttata tatatttatt cacttataat cactgaaaaa tcgacttttt
    16501 tcaaagtaaa ttctttttga attagtacta atctgctctt tattaatggc ataaatatga
    16561 ttatcaagta ttatcagtgt tataacattg aattttttat tcttcataaa tattgccccc
    16621 tttatattac caactaaata taatatatct tatgttactt acatattctt taagaaattc
    16681 tgaagatttt ataaagtttt ttgtattata attaattaaa aataaaattc tagttataaa
    16741 acatattttc cattaaacaa aaaaatagtt aaaataaatt cataaccaca taaacttatt
    16801 ttaactattt gtatatttta attataaata ttaacttaaa tttttaacag gatacactca
    16861 taaaataaac ataatgaaca acaaaataat atgtctcagt ttctttatat actttacctt
    16921 ctttcaattt tctaccatgt agacgtataa gataatatta gtaattcact ctattgctta
    16981 actcgtgcga tatggacaac caagaaagct atttcctcat ttgttaactt ttttccatat
    17041 ttcttttcta aaaaatctct aacttttaat gcaattaaca tttcttttgg atttgacctt
    17101 ttcatttgat taaataataa atcatcacca ctgcttaaca tactatctga aaaaaatctt
    17161 tccacaaaaa attttatatg tgttataaat ctcatataat gcatactctt tttatctaag
    17221 gttctattca aagaaaaaac aaaaatatta attatttctc caattacttt ggcatactta
    17281 atcgtatcat atgaatttcc atctgaagcc atagcatttg caaaatggaa agcaatgtta
    17341 gctgcttctt cctctggtaa ttgaatgccc aatctctcct ctaccatata aattgcaacc
    17401 attccaacct taaactcatt tggataataa ttttttattt cccaaaatac tcgatttgta
    17461 atttttattc ctttacgcat cctttcaatt gcaaagttaa aatggtctgc taatattaag
    17521 tataagtttt tattaaaaga tgagttcaat aacttttctg cctctactat tatttcctgt
    17581 gttatcttta aaatgtcgac aggaatatca tccattaatt ctaaaaattt ttttgatttt
    17641 gtattttcaa cagggacaaa catttgatta tcattggaac cacaaatata ttctcctgtt
    17701 tttttaccat atccaattcc tttccctagc aaaataaatt cattatactt tgcatcatga
    17761 actaaaacaa cgctcgagtt caaaactttc tttattatgt agttcatttt atttgctcac
    17821 cttctattgg tcatttaaaa tctatactat tattttcaat tactttttta taccaataaa
    17881 aactatcttt cttatatcga ttatatgtcc cctttccata atcatcagca tccacataaa
    17941 tatatccata acgttttgac atttgtttcg ttgattcact gaccaaatca atacaacccc
    18001 atgaagtgta tccccaaagt tcaacgccat cttcgataat cgcatctaac atacatttaa
    18061 agtgttcttt taaataatta attctatatt ggtcatgaac ttttttatct tcagttaaaa
    18121 tatctttagc tcccaatcca ttttcaacta taaataaagg ctttctgtat ctatcatata
    18181 attcaatcaa agatatacgt agacctattg aatcaatttg ccaaccccat tcactcattt
    18241 ctaaataagg attttttaca gctaataacg tattcccagg tgtaatatct aatccagttg
    18301 tatcagctgc ctcacaagaa gacatatagt aagagaatga aataaaatct actggatact
    18361 ttttcataat ttcatcatca tgttcttctt ttgctatttg aatgttatta tttttaaaat
    18421 acgaaagtaa atatactgga tactcaccaa aaacctgcgt atcactatat gcataaatac
    18481 ttctcatttt ttgttgagtt gccaaaacat cttctggtct acatgtatat ggataatacg
    18541 ttaattttgt taacatacac cctacttttg aatttggaat aatctcatgg caaatttttg
    18601 ttgcaagggc actagctaca aattgatgat gcatagcttg aaaaataact tcctcaaagt
    18661 ttttatcctt aaaacggtct cgaactaaac caccagtagt gtacggatga cgaatcatag
    18721 aatcaacttc attaaatgta agccaatatt tcactttgtt tttatatctt tcacagatag
    18781 tttctacata cctaacaaac atattgataa cttgtcgtga ataccatcca tcatagttta
    18841 aaacgatatt taatggtggt tcataatgtg acatagtaac aagtggttca attccataac
    18901 ttatcaactc atcaaataca tcatcataaa actgtaaacc ttcttcattt ggctctttat
    18961 catctccatt tggaaaaata cgagaccaag caatactcat tcgatatgtc ttaaatccca
    19021 tttctgccat tagagcaata tcttctttat agtgatgata aaaatcacta ccatgacgtt
    19081 ttggataatt tacttcatct tcaggatgtg ctaatccttc ttctatatct tttgttgtga
    19141 tttcattatt agatttatag tctttcacat ctgcatcaaa gtgtgccttt gctacatctg
    19201 atacagacca ccctttacca cctaaattcc aaccaccttc aaattgatta gcagctgtag
    19261 ctcctcccca aagaaatcct tctggaaact ttctcatatt tatattcctc cctttacatc
    19321 aaacaagata cttccttttt ttacattgcc ataagactgg tttaacactt tataagcata
    19381 atctgctcca ttattgttta caatcataac agttgaatct aaattagcat gtgagatttt
    19441 gtctaaatca aattgaatta ataaatctcc ttcttttaca ttatcgcctg tcttaacaaa
    19501 tgttttaaat ccatctccat tcatacttac agtatttatt ccaatatgaa tcaaaatctc
    19561 agccccattt tttgttctta aaccaattgc atgttttgtt tcaaataaca tcacaacttc
    19621 tgaatcacaa ggtgcgtata aatttccatt ggtcggtata attgcaactc catctcccaa
    19681 agtcttactt gcaaatagtt catcattaac cttacttaaa tctattactt ttccttctac
    19741 tggagttact aagatattta cttctttttc ttcttctagc tcttcttcaa caacatctcc
    19801 aattgatgac tctactgtat ttgtatcttc cttccaaata atcagtgtaa taataaacga
    19861 gcctaatatt gcaataacaa aaccaatact cgcaaaaatc aaattgtttc cattgtttgg
    19921 gtcaataaac atactcatac tggcaagccc tggtccaaca attacaaatc cttttactgc
    19981 aaataatcct aaaaatgtac cagaaattgc acttgagaat acaacaccta acattgctct
    20041 tttatgttgt aaagtaatac catataaagc tggctctgta attcccatca aggcagaaat
    20101 tcctgcagat aatgctgttt gttttaatgt ttcatctttt gttcgcaagg ctactgcaaa
    20161 acaagcacca ctttcagaaa tattgtgggc taatgaagat gtcatatata aagcttcata
    20221 acccaacttt ccaattgtat taattgaata aggtattagt gctttgtgca ttcctgttgc
    20281 aaccataaat ggaagaaaag cagcaagtaa tcctacaact aagaatccca ttttgttata
    20341 taagaataaa ataatggttg taaaaattgt accaacgtta tatcctaaag gacctaaaat
    20401 agttagagta attggaacag taattgccaa agacatcata ggaacaaaga aagttcgaat
    20461 tggttttggt gaatacttgt taaaaatttt ctccattgtt gccaaaaaag acacacataa
    20521 gatagccggg aaaacttgtg cattataggc aatgtttgga attgtaaatc caaatagttc
    20581 gacaccttca cttaaagcag ttgttgttgc tggaagcaat aagtatccta cagcggcaat
    20641 agctactaaa cgattacatt tcaatacatt ggcagtagtt acagctacca taagaggtag
    20701 gaaatagaat gttgcatctg aaatagcaac taataatgta tatatagtag aatctgattt
    20761 taaaatatta aacattgcta ataacatcaa cagtgatttt aaaataccag ctcctgcaat
    20821 agcaaaaatc agtggttgaa aaatattaat aataaattct ataaatcttg ttcctaagtt
    20881 tattttttct tcagttttta cagtactatc atttgtatct aaagggcaaa cttttaaaat
    20941 ttcatcatac acatcaacaa cactatttcc aatgacaact tgaaattgtg ccccattaac
    21001 tatactcata acaccttgta ctttttttaa agcatcctga ttgactttac ttctatcaat
    21061 caacttaaag cgtagtctag ttgaacagtg ttctaaagct aaaatatttt ttgaaccacc
    21121 aacttgttca ataatgtcaa tggcagtttt tttataattc atcttgtaat ctccttctcc
    21181 ataatttttt atgaatggcc attcaagatg caaaaaaggc agaacctata taaacacctt
    21241 gaaaaatcaa agcaatatat tcgatcctgc ctgcattacc agtaacatgt cttctattta
    21301 tactattttg tattattata ttaacatttg cctaaaagaa ttgtcaatac ttttttaaat
    21361 tttctgatac taactaatag cacctttata attcattttt tatgtcaatt aaagtaacag
    21421 tagccaccca ccaattagta tcaaaattga acctggtatt ttttctttta tattttcttt
    21481 gaatataaaa tgagcaagta ttaaagttat tatcatagat acttgagaaa atggctctgc
    21541 tatactaagt tctagattat ttaaagcaat taataatgct aaataagaat atccattaat
    21601 agcaccactt aaaattgaaa gccccttttt attactaaaa atctcacaaa ttattttttg
    21661 tttcttttta aatgctaaaa agataaataa atttaaacct acgaaaaaat acagtatagt
    21721 tgaatataat ataggagata cttgtacaaa aaaagcctta tccaatactc ttcctactga
    21781 ctgcaaaaat atgtataaaa acattaatct acatggtaaa ttgttaaaaa ttacagagac
    21841 attctttaaa aaagagctta ctttttttaa tataaacact ccaccaatca taattatgat
    21901 tcctgcaact tttgctaaac ttaaaccttc acctaaaaat ataactgata aaagcagtaa
    21961 aaacaatgaa tttaaactat ttattggagt aacaagtgaa acttccccag ttgctaaaga
    22021 cgttacatac gctactgaac caattgaata gactaaacta cttatataac atggtattaa
    22081 aaacgaccaa tcactaactc tatccattat gctaaatgga agtaaacata ctccccctat
    22141 aaagaaaaat agaaatgatg aagctatatt tttataaata tctccttctt catcacctaa
    22201 taatcttaca actactcttt caaaagctaa tagcactact ctaattaata atgcaaaata
    22261 agataatatc atttttgacc tcgcttaaaa attaatccga cttattgtta tattaactgt
    22321 ataagactac catataggta agtctttggg attttttaat attttatgtt taaatacagg
    22381 tacattaagt ttttcaccta gtttacggca ttgctcttta taggtacctt ttaatttatc
    22441 atgaaaaact atacaatcaa ttccaccttc aatactattt atacactttt ctagtttctc
    22501 aaatcccgat tcatcatcat acattccact tacaatccat tgatttggca atacatgtcc
    22561 aattgatatg ttatattctt tattttttat aatatctgag aaagatttct caaaatgtac
    22621 ccctccaaca cataataaag tcttcaaagg ttcatcacaa tcaaatactc taattaatga
    22681 ttttgccaat acctgtattg ctatactatt attaaaacta tcactactac ttcctatctc
    22741 aatatccaac ataggtacag aatattctgt aattaattta gattcttcac catatgttgt
    22801 tccagaccag tgagttgctt ctgttaatgt agtaaattca tcaagtagat tttcattttt
    22861 tatatcctca atagcattga ttagattttt aaagtatctt ggatttgaat ttccaaattc
    22921 tcctgttgga acatctccaa ttgtatgagc tgttaggata tgttctggag cattttctcc
    22981 ctcatgccaa tttacaactc ctgcaaaatc ataatctctg aaatgtctat ttaaaattgg
    23041 aagataatat ttataattat gacttattac atcttttgtt tttacaaaat gaaataagtt
    23101 attgttttta tccttaaatt ctaatacaaa attatcatca ataacaatat cagtttgatt
    23161 taaatccatt atttcttcac aacactttat cacattactg gctactgggt ctttctttaa
    23221 atcactacaa aagaaaaaaa ctgctttttt attattcatc cataaatcac actcctataa
    23281 cttaaatata atatatcttc atacctaaaa tcatctaaat atcactctaa ttccattaaa
    23341 ttatttatat ataatatact accatactca ttttagctat acctttgcct gagtttctat
    23401 atatatgtga tacatcagct ttaaatctta tagaatttcc actagataca atgtaatctt
    23461 ctccatttat tttaatggtt acttcgccac taaaaacagt tataaactct tgtgttcctg
    23521 gaagttcagc atttgaatct aaatttccac cttcttctat ttcaatcata aatatctcaa
    23581 atcttctttt ggagtcaaat ggaaatattg gataacttat atactttcca ttatcttcaa
    23641 taaaatggtc tatatcactt ttttgaatca attctatgtc actttcctgc ctctccatca
    23701 atgatgtaaa tgagattttt agtccattag ttattttctt caaagtagat atagtaggat
    23761 ttacctcaca cttttcaatt tgagatagca tactcttact tacaccacta agttgtgcaa
    23821 tcttgtctaa actaagatgt ttttctttcc ttatcttttt taaattttca gcgattattt
    23881 catttaaact ttgcataaat accccctaat acatactaat ataagataaa ttattttgat
    23941 tattaaataa acttagtaat ttaatagaaa ctttttatcc attatactgt acattatgtt
    24001 taatatacat tacttttttt agaatgtaaa tagttttctg aaattttgtg aaagtgaaca
    24061 aataaaaaac tagtaaccta tatttttaca aggctactag cttttattat aataatattt
    24121 cattttaact ataaaactta gatgcaccta taagctgtct cttagaaaca atcttagcta
    24181 ggtttgctaa tgaagaaatt tttatatctt taaaagaaac taaaaataca aacttaaaat
    24241 ctgtaaattc ttgatttata ttgtatatat caaaataaca tctcaaatta tcaaataagt
    24301 aattatcttg taagtctgct tctatctcat cagtagcatc ttttaaatac gctcttacat
    24361 cttcaaataa aacactgaaa taagactcat ctatttcttc acctaactct tccaatattt
    24421 cttctatatc taaatcatcc atatcaaaag aatttcctgt caactggcaa tcaaatacca
    24481 atatattttc ttgatttata gtatttgata tatcttgtac atattcgaag tctggacgta
    24541 attctaaaat gtcttttatt tcactgctct ttacatggat ttttcctttt ctattttcaa
    24601 tttttaaatc tatcatatgc tccccctttc aatctacaaa tctttaaata ccttgtttta
    24661 taatcttttc aatatctatc caattactaa ctctaactat attttgatgt ttaacatctt
    24721 tattatagtt agtatccaga agtattactt taacaccatc atcagctagt tgcactgagt
    24781 ttaaaggatt atcttctata aatatgtcac aactcagttc tcttgccttt tcaactttat
    24841 aatcacttcc taataagtaa acatctatat ctgaaaatcc ttgtctcgat agccaatttt
    24901 tagttacatc agttaaacac tcatgtctag ccgttacaaa agataaatta tgtcctttag
    24961 ataatgtgct aattacctct gtagcaccct ctacaatctt tgcttcatca tatgagtgta
    25021 tatacttatt attaaactcc gcatacacat ctcttattcc cgaaccatac aattcttccc
    25081 aattatgtgt tatacactct gcttcagtta tatttttatt aaagatatca tttaggtatg
    25141 gaagaaaatg ataagggctt gttattgttc catctatatc aatacatata ttcaatttgt
    25201 tcatttttac ctccatgtaa catttattga tttgattaat caaatattat agcacagctt
    25261 taattataaa tcaaaactct ggatatgact atacctatat aataccaaaa agcttgcata
    25321 tttaagcaag cttttcgtaa attttacatt tttaactaat atataagttt ttctaaggtt
    25381 ggttccactc caaattgtga tttcagttct ttatattctt ttaatgcttt gtcattattc
    25441 gttttagttt ttatcgctct tatcattaaa ttttttggtg tatcaagtgg agatatgtat
    25501 tctactacag aagtatcata tccattgccc tccaataaca atgttcttaa accatcagtt
    25561 atcaaatctg caaatctagc tttaaataca ccatgtttta atattggttc catagcttca
    25621 tatctatatt gccctaataa ctccttatga caacatggta ctactactat agcttcagat
    25681 tttgctctaa ttcctagacc tattgccata tctgtagctg tatcacaagc atgtagactt
    25741 attacaaggt caacatccct attaggtgta taattagtca aatcctcttt tataaatgac
    25801 atatttttat aacccaagtt tttagccata ttttttgatg cttctataac tacatctgaa
    25861 taatctattc ctataaaata acagtttttc ttcaatactt cttttatata aaaatttaag
    25921 acaaagctta agtaagattt tccacaagca caatccaaga tagttataca gtctttatcc
    25981 tttatttctt ttaaaatact atctatcaat tcaacaaaat ggtctatctg attgtatttt
    26041 cttattttat cattttttat cttaccatct tttgtaagta ctccaatttc ttttaaaagt
    26101 gcatttgctt gtcctacttt tacataataa tctctgtttt ttatttgaga tgccgtaaat
    26161 tcatctattt taggtacttc ttctttatta tctaaatatt ttacatttac cttcttattg
    26221 tctccttcaa ttacaacaat agttcctcgc tcaacatatt taaatactaa tccatcataa
    26281 ttttctgcct ctttagaaat attcttaagc aactcattaa acgtatataa atctgatttt
    26341 cctttaaaat taaatttata tttattatct tcctctattg caattccttt aaactctttt
    26401 cttccagatt taaaaatcac ctctaactca ataaaaaaat gcctattatc aaggtttctt
    26461 tgagtaatcc ccataaaaaa catatttagt tttcctatac tctgtttatt catcataatc
    26521 tcccttttaa cttatatctt taaatcttat taattaatat tatttgtttc tgaattattt
    26581 atttcttctt ttaaatcgtt caatctattt ttaaaacttt ctatctgctt atctgataaa
    26641 tcaagatatt ctgttatctt ccctatatat ttagggcttt tagtatttat taatttcaaa
    26701 gcactttcca tagatttttc tctaagctca tatgctttct ttacgtcatc tctagcatta
    26761 ctcaatgttt ctgtatactt ctccttagaa agttctggaa tctgcatatt ttcatattct
    26821 tttacaactt ctttacactt gtttctggca tttgtaacat ctttttttat actagataat
    26881 ttagttatat tttttgctat atccttagtg taaggtatac attctctcat tggaactaaa
    26941 gctgctcttt ctatattata aatttctttc ttaaaaattc ctatactttc catatctatt
    27001 ttttttatat cttcactatt tttttcttta cttgtatatt tattattttc ttttgtatta
    27061 tcttcttgtt tttctgattt attattcact gaaacattac tatcagaatt attcttttct
    27121 tttatatcat tacttccttc ttcattactt tctttatttt gtatatttct atcttgtttg
    27181 ttgtttaaat ttttattatt tgatatttct tcattctttt tattatcctg ttttaaatcg
    27241 gtactttctt tttccttaaa atttgataca tacttttcat catatgtctc atcagaaata
    27301 taattgtgat aatacataga ctcttttatt ttattaagtc cactcatata aatattactt
    27361 atcattattg ttgtagctaa taccacataa atactactgt agatacaaga cctcttaaaa
    27421 ttcttgtaat ttctgaatat cactactaaa ggaataagat ataaaaatat aaaaactaat
    27481 atccacctca taagtcgtgt ctcctttctc aaaaccgaaa tccctcaaat atttattttt
    27541 aatcatgtat cttatagtat ttaccaattt atgtttttta atcgtgaact tttattaaat
    27601 taaattctgt atagtcacaa gatgctaatg tatattctac tccatttcga atccccttca
    27661 atcccatttt aaaagattct ttaccatgca agtgaccata aatcaccttt tcaacattat
    27721 attcttcaaa taactttgta aataaagatt cttccaattt atcattcgta ggaggataat
    27781 gagtaatcac aattattttt gagtgaccac ttttacgagc agattctaat gatagtctaa
    27841 gtctatgctc ttctctttta taaacttttt catcagtctc atcaaattta acatcattag
    27901 gacatatcca gcccctacct ccacaaattg cataatcttt atattcataa aaatttgtct
    27961 gaataaaacg catgtcttca tatagcttat ttaaacttgt aacggttgtc caccagtaat
    28021 catgatttcc ttttataagt attttctgac ctggaagttt gcttattata tctaaatctt
    28081 ttttagcttc cgataaatta attccccaag atgtatctcc tagtactaag accatatctt
    28141 cttccttaac aacttctttc caattatcta taattttctt ttcatgacca tcccaattac
    28201 ttccaaagat attcattggc ttattgactg atgttgaaaa atgtaaatca cctatagcat
    28261 acaaactcat aatatcattc ctttcttaat tacactaaat aggctgtctc tttttagaaa
    28321 ttattttcca ttaagaggca gccccattaa tatttagcaa attttctaca agtagttttt
    28381 tactgaacta ctttatcatc taccttagta tccttatatt gctctagtaa tatatctatt
    28441 tcttgtttta attccaaaat ttcagttata tttttatctg tttcagttct atcaagtttt
    28501 ctttggtact gcatagcttt atgtgtatca cccaagtcat ataactcttg tattctattt
    28561 agagtatctc taatagctat agacttcatt tggtacatag cctgagcttt cattatgtcc
    28621 tctcttattt cactcatttc ttggtctaaa agaaaggcag catcagatgc tcttctaagc
    28681 ctttcagcaa gagtctcagg tatgtaatgg tcatatttat attttaatga atgttctatt
    28741 gtagcccaga aattcattgc aagagttctt atttgtattt cacatataat ttcctttgaa
    28801 cccgcaatgg agtttatagg gtattttatt ataacatgat agcttctata accactatct
    28861 ttgtaatttg taatataatc tttttctcct attatagtca tatcatttct tacttttatc
    28921 aaatcaacta tagcatatat atcctcaaca aactggcaca ttattcttat accagcaata
    28981 tcatcaattt cagtttctat atcttttgca tttatccttt ttaacttaga tattatggaa
    29041 gatatttttt ttgttctgcc tgtgacaaac tctatgggag aatattcacc ttttgctaaa
    29101 tattcttttc ttatattttt aaattttatt ttcaattcct ctactgcatg ttcataaggt
    29161 gctagtatct cattccattt ttcgtattcc atattgctca ccctttattt gttttttatg
    29221 accttcttta ttttatcata atttattttg tttttgtata gtaaagcaat tctccatttt
    29281 ccaatatata ccctatttct tctaaatcaa ctaagtaact tatgacagat actaacgaag
    29341 atttaaaaaa atgatactct ttataattat tgcttaaatt attgttattt ataatgtttt
    29401 ttaataaaat ttctaaagat ttcggttcta caagcaactc tcttatttga tataaatatt
    29461 tttttaaagc attcaaatgt ttttctatta atttataaga atcttctttc gatataactt
    29521 gcttgccatg ccctaaaact aagtactcaa aatctatatt ttttaatttt tctaatgaat
    29581 ttatttgttc ttgtatatca tatagaaaca agaaatcgta ttttgataga atttcatctc
    29641 ctatcaataa atcgccaaca aaaatcacat tatccttcgt caaaacagct atactaccag
    29701 gtgtatgacc ctgaaactca attatatcaa acatttcatt gtttaattca atttttccaa
    29761 cttctataga tttatctatc tttatatcat ccaacatttt attttttaag atattatcca
    29821 taaatacatt gctctttccc ccaacaatat actttgaaaa caattctgga ttttctatat
    29881 ataatttgtc gtattcagat gacattatac acacatcttt ataatattct ttaaattgat
    29941 tacaagctcc atagtggtca tcatgaccat gagtatttat aatatatcta acctgtatat
    30001 tattttcatc taatacttta tttactctcc taggtcttga accacataac cctgggtcta
    30061 tcataagtgc tgtgttatct tcaaatacat atattcctgt attcgtgcca ccttttataa
    30121 aaaaggtatt tccttttact tttgtaagat tcaccttcct cacctcattt tgacaatatt
    30181 tatgatacaa tttattactt actaatataa aactatacaa gaacaattct ttttattcta
    30241 tctatagttc tttttcttat tttgtattct ttagatatcc ttattttcac attatatgct
    30301 aaatttctat aaagtaatga ctctataaaa tattcaacta tacctctatc cctaaagtct
    30361 atatctatat ttccacttga ataaatattt gagtctaata tcataagctc atcaaaaaag
    30421 cttaatctct tagatttact taaataatct ttatttggaa taaatctatc acaataatct
    30481 atcctgtaat ttactacttc atcatttatt tcaaatccat attgcttttt cattaactct
    30541 ctatagttat tatttttctc aataacatta tctaaatttt tattataatc ctcttttttc
    30601 caatgtccaa acatcctaca atttaatggt ctaacttcat atattagaca tctattatta
    30661 gtatctttaa atggacatgg gttctttctt gtatactcct caaaataata atctaatatt
    30721 ttacttatac agcttttttt taaatcaact ctatcttcaa gataacaaaa tatattcaaa
    30781 aactcaatta aatttatacc tactgactcc atacaacaat ttccacatcc agaacaattt
    30841 cctactggca aggacttata tacatcattt agtttgtcaa agagcttatt tttaatggaa
    30901 taatctatac aatttaaaat atctttattt tttatactag tcattattat tactccttgt
    30961 tataattatt aagttaatac aaacaattat tataacatat tttttaaatt taatcgcata
    31021 tggaggtatt ttatgaatag atatacaaaa ataattaata tgatgggaag ttattacaca
    31081 aaagactttg aaaaagaaaa aaagaatgta ataaaagtta gagaagttaa agaagataca
    31141 gttagaaaat ttttcttgca aggtgactgt gaagttcttg tagtatttga agacactggg
    31201 aaagaaatac ttattgatga cttttctcct gaagaagata tcaaaaaata tttaggaaca
    31261 aaatttataa ataaaaaaag atagttatca aaaataattc taaaattaaa atttaataaa
    31321 tgtacagtta taattctata taattatagt taaaagttat cttttattgc ttttttagac
    31381 tttaagatag ctttttttct attttttgta aaataattcc ataattcaca ttaataattc
    31441 acaaaatgat ataatataac aaatagataa attttaaatt tctaaataca taattaatat
    31501 tttgcgaggt gattttatgc aaatttattt tagagttaag gctgttggca aacgcaaacc
    31561 tatgttagaa cttacccctt ttgatgtatc tgacaacatc aattgcctaa aagaagctat
    31621 ctctgaaatt gtatctaaaa atgttcttga atataatgag aaaatcccag aaaaatcaat
    31681 aataaacttc ttaactaatg aagaaattga atctcaagct gatattggaa aagttggatt
    31741 tggctctatt tacaatgaga ataaacaatc tgtagaaaag gcaatagaag tagcgttaca
    31801 ggcatttgaa gatggaatct acaaagtgct tataaatgaa gatgttattg aaaaactcga
    31861 ttcccctata aatctgaaaa gtggagatat atttactttt ataagactta ccttgcttgc
    31921 tggaagaatg ttttaaaata aataatttgg aggtacataa atgttagaac gtactagagt
    31981 agaaaaaaat cctaaaataa ttgaaaattt taaaaataat tttactaaat cttcttcaaa
    32041 acagtactac gacttagcaa aatctctttg ggactgcata gaaaatagtt catactatta
    32101 tagagaacct gaggacaaat tcttaaatat gataaagaaa tactacaatt gccactacta
    32161 cgaaattaaa aatatggacg agctcctttc tggaccttta aaaaatgcat tagaattaat
    32221 atttacttct gaacaagtag aagaactttg ccaattaatt aaaagaaaga tggattatac
    32281 atattcagga ggatattatc gtaaagggta tcgctcaaat aaacttgtgg attattttag
    32341 aggtattata aggtttattt caaactatgt tgatttaatt tattatgatt tttcagttat
    32401 agattatctt actcttccaa ataaggtctt gtactatgac tctataatga gtgataaaat
    32461 agcactagaa atagacaaag gaaatcaaaa agtaacaagt gccttagaag aagtaatatt
    32521 tggagaaaat caaactgctc tacttacaag acaaatgata attggtatca tgaagagtca
    32581 tagtgataaa tgctatgaaa tgctttctaa acttcttttg gcagccaaac aacaagaagg
    32641 acttagacag caaattgtag agagtttaga tgagggtaca atttctgcat caatatacat
    32701 gttaaaaact attttggaca acaatttaga gcgatttagt tctgttataa gagccttgga
    32761 tacatggaca ggacttggtt tttcagacca aaaacctgga ataatacgta aatgtttaga
    32821 actttcatat aaatcattga ctgatgaaaa gttccgtata gaatcaatta atagcaatga
    32881 taatcttgaa atttattttg ccttatgggc tactgcaact tacaacatat atgactctta
    32941 ctctcttatt gaaaacttac tttcttgtca ggaaaaatat aaaaaacttg tagctcttta
    33001 ctttttatcg catacagaaa gtacatattt taaacattct atagcatcta aaaacattaa
    33061 gggtgatgat ttagagatac tagcatgggt aattacaaat ttctatgtag aaggttctgt
    33121 tggctactac tatagttggc aaaacagaga aactaatttt gacttgtcaa acactcctat
    33181 tgacaaagac tttgatgcta gaaaagaaca atttgaaagt ttaaaacata ctttagaaat
    33241 tttaaattca aagacaaaat cattttcagg aagtgtattt ccatggactt ggattgaaat
    33301 ttcttctcat gaaatactta aatgtatgat gactttaaca gcatacgata tgaatgctca
    33361 atttatagat gtgcttatgg aaaattgtca tcttatgagt tcagatttaa gagcttcact
    33421 tatagtcaat tttgtaaatg aaccaaagac agatagacaa cgtgaatttt tattggaatg
    33481 tttaagtgat aagagtgttt ccaatagaga aacggtattg agtttactta gaaagcttga
    33541 gctaacagat gtagaattag ataagataac caatctattg tctttaaaga caagctctat
    33601 tagacaaaca gtaataaaaa ttttacttga acaagatgat tcaaatttgg aaaatattgc
    33661 ttcttcactg ataaaatctt caaattctca aaaatgcctt ggaggtcttg aaattgtaaa
    33721 cttattaaaa gacaattctg atagacaaga actatataat aagcttattt tacttataga
    33781 ggatattgat aaatctaaaa agaatacatc tcaagttcaa atattaattg ataatttgtt
    33841 aggtaaagca aaaaatgatt actgcaaaga aaatggtttt ggaatatata accctgaaaa
    33901 caaagttcaa attccagaac aatttgattt taaagatgat tctaatagct catcaaagct
    33961 taataaaatt atttcaaata taagtttatc tactagtgag gattctgttt ctgtagcaac
    34021 aaaaaaagaa gcaaaaaaaa ttctcgatac ggatataaat aaaataataa aagtttatga
    34081 aaaatttgat gagcttatta ctgaaaatgc agattatgaa tatgaagttg aaagatggtc
    34141 aggtaatgta gacaaagtgc ttttaggtta ttctacttat aacttgactc ctattaaata
    34201 taaagtttct aatgatactc ttgataatta tccattggca gaactttgga gagaaactag
    34261 taaaaagctt gacttagatt acaaattaat acttgatatc ttattttata gtataagtac
    34321 atgtggatat aatgaaacag aaaaaagttg gttttcatct atatattcaa atgtccttga
    34381 ctctaaaaaa caaaataaaa tatatacaaa acttgaaaaa tttaaattta atggacttac
    34441 aagaaaactt ttaagagttt tactaaatga gtttgaccat aaagaaatat ttaactattg
    34501 tttagaaata tactataaac ttagaaactt attgactata gaacaatgta aaaaacaaag
    34561 agttgaattt actaaaaact attacagaca aaatgaatac tgccctgctt taaatgcccc
    34621 tattttaaac tattggcttg ataaagcaca ttcgtttatt tatgatgata tttcttttac
    34681 taagtttttc tacactacgt tttcaaatta tcagttgcta gaatataagt ttcctttcta
    34741 tactagcata aatgtgtttg ctcgtgcaca tgaattaggt ttaatgactg aagagcaagt
    34801 taatagagaa atgataagtg gatacaacgc atcaaatata ataagaacat taacaaatcc
    34861 tagatgggct ggtgacacat tagaaaagta cccttggctt aaagataatt taaaaactgt
    34921 tacaaaccga attatagaaa ttgaatcacg tagaggtgat atgccaacag aagtgtctca
    34981 tctagctaat aatatacaat actttgaagg tatagattat tttgtaaata ttttggtcgc
    35041 tcttggtaaa gaaactttcc agagaggata ttcatatggt agcaattatt caaaaaaaga
    35101 tgttttaagt tctctactta aaaattgcta tcctactaaa gaagatgatt caaaaaaatt
    35161 aaaaagttta cttaaaaata ccgacattac tgagttaaga ttaatagaat ctgctatgta
    35221 tgcccctcaa tgggtaaaca taattgaaaa atatttaaac tggaagggtc tgaaaagtac
    35281 aatttggtac ttccatgcac atataaatga aggcttctct gcacaaaaag aatcagaggt
    35341 tgctctattc tcccctattt caccacgtag tttcattgat ggtgcatttg atgtagattg
    35401 gttcttagat tcatataaaa ctattgggga agaaagattc aatatacttt acaagtcagc
    35461 taaatatata actagtggtg gtaactctca tagacgtgca caactctatg ctgatgctac
    35521 acttggtaag cttgataaag acatgttgat gaaagaaatt gaaacaaata gaaatcaaga
    35581 taaattgaga agtttctctt tacttcctat gaaaaaagga gacctaaaag aagcaacttt
    35641 ccgttatgaa tttattcaaa agtttgctaa agaaagcaag caatttggtg cacaacgtcg
    35701 tgaaagtgaa tcaaaagctt gtactatagc gctagaaaat ctagctagaa gtgctggatt
    35761 tggagatgta aatcgtatga tgtgggctct tgagactgaa aaactaaatg aagttgctca
    35821 tctatttaaa ccaaaagata ttgatggtac ttcaatttgg atagatgttg atgaaagcgg
    35881 aaaagcatct actaaagttc aaaaaggcaa caagtcttta aagagtatac caaaagctat
    35941 tgctaaacat ccatatgtac ttgaacttaa atctgtccaa aaagatttaa agagtcagta
    36001 ttctcgtgca aaacagagtt tagaacaagc tatgaaaaat gaaatagtat ttacagttaa
    36061 agaaattgcc aatatctata aaaatccagt tatctgccct ttgattaaaa atctagtatg
    36121 ggtatgtgat gataatattg gatatgttta taatgataag tctaagttct ttatttgcaa
    36181 tatagaaggt gaaacttata agttgacagc aaaaaataaa ttaaaacttg cacatccagt
    36241 tcatttgttt gaatctggta agtggagtga gttccaacat gatttattta gtaagcaact
    36301 tgttcaacca tttaagcaag tattccgtga gtattataga ataaattctg atgaaatatc
    36361 tgaaaaaact atatctcgca gatatgctgg tcaccaaatt cagccaaaga aaactgtagc
    36421 acttcttaga aatcagcttt ggactgtcga ttatgaagag ggattacaga aagtatttta
    36481 taaagaaaat atagtggcaa agatgtttgc acttgcagat tggttttcac cagcagatat
    36541 agagcctcct actattgaaa cagtagaatt ttttgataga aaaacttata aaagtttaga
    36601 attagcttct atacctccaa ttctattctc agaagttatg cgtgatatag accttgttgt
    36661 aagtgtcgct catgctggtg gtgttgaccc tgaaactagc cattctactg tagagatgcg
    36721 tatagcaatt gctaaagaac ttttattatt attaaaaatt aacaatatca attttgaagg
    36781 ctctcatgtt cttatcacag gtaatttagg tgagtattct gtgcatatgg gttctggtat
    36841 tgtacataaa aaagggatgg gtgctttgaa tatacttcct gtacattctc aaagtagagg
    36901 taagatattc cttccattcg ctgatgaaga ccctaagact gctgagatta tgtctaaaat
    36961 cttattactt gctgaagata ataagattaa agaccctagt atcttaagca acttataaat
    37021 ataagattta tctctaaaac atattgggct ttctaatttt ccatgaaagt ccaatatgtt
    37081 ttatattata ttttatacga attaatttat aactatagca aatttatatg atatcttcat
    37141 catacatatt gtatatcact gaaaattttt ttcttcatta aaacctttta accctataaa
    37201 atctgtatag aaaatctttt tctaaagatt tatttaaagt agcactttct tgaaaattta
    37261 taaattttat ctctaaagaa gacttattta taggtataac cttatgtata agtcttaaat
    37321 ttactatgta ttgtctatga gtcataaaga aattaggagg taggcgtttt tttaaatcat
    37381 caaagttctc ataaaatttt atcgtatctt catctctagt atataaatta atagctcttg
    37441 aatttttctc aaacatgact atatcatcaa aattaatcat atgtatctgt ttccttattt
    37501 tatatacaaa aagagtatct tctataaaat tattttttgc aattttatga gagttaatgt
    37561 ggtctattgc tatattaatt gaatcaagta ttttttcttt tttaaacggc ttaactataa
    37621 aatctatagg atgtacttta aagctctcaa gactatagtc agcaaaacct gttacaaaaa
    37681 ctatatgtat atctttatct atagaaagaa tttctttggc agaatcaatc ccatttttat
    37741 atggcatatc aatgtcaata atcaataagt ctattttctc actttttata aattcaattg
    37801 cttctatacc atccttgact ttcttaacat aatctacata tccggactct ttaactatat
    37861 ttgctatatc atctcttaca attttatcat catcacaaat caaaactttt atcacagact
    37921 tctctcctcc taactaataa tattttggta ttaaaattac aaattcagtt aaaaaatcgt
    37981 cactatttac aagtatacta taatcatact tatctagtat atttttaact agatataacc
    38041 caaaacctct gtcttgccct tctttagtgc taaatccatc caaaaatata ctttcgacat
    38101 tatgtatttt aggaccatta ttttttatgg ctataatata ctgaaattca tcttcatatg
    38161 catgtaatct tatatacttc ttttcctttt gtatagcaga caaagattct attgcattgt
    38221 ctattatgtt taatagcact gaactcatat cattaggttc aatctctaaa ttatccataa
    38281 aatcttggat atatacatca aaagatatgt tcttttcaag acattcctca tattttaagg
    38341 aagctatagc atctaaataa gatactttcc catatcttga attttttgat ttaaagctat
    38401 ttgatatatt cgttatatat tcttttgcat cttcagctaa cccagtattt aatagagaat
    38461 atactacttg taaatgtttt aaatagtcat gtttttgact tcttaatttt acgattatat
    38521 tgtccttatg atttatttta ctgtctaatt tttgtagctt atactcttgt ttattgttat
    38581 tgtctagtaa tcttataaat ttaaatccta tataaaagct taaaataatt aaaattaaaa
    38641 aaagtataat atttaatact ccttcaagta aaaaattata tcctaaactt aattgtatat
    38701 actcaatacc ctccatgaat aaaatccata caaaacacga tacaaaaaac gttaatattc
    38761 ctctttttaa tatgtctaat tctttataaa acctgtattt attcattata agattactaa
    38821 atataaacaa agttagtatt actataggaa atataaaatt attctccata attcccctcc
    38881 catctacact ttatatgagg attatatcat tttagaaaaa ttatgtaaaa agtcaaaatt
    38941 tacatgtaca ttaattaaac ttgcaaatac acatataaat ttgtacaatt aagtactcaa
    39001 aacttgaaga tacttacttt gatatatatt ttaaataaac atataattga tttagctaag
    39061 aaaggaagtg gtaaatatga aagataattt aaaaatagca ctaagataca taataagtta
    39121 taaagcaagg tctttagcaa ttgctctaag tataattctt gctacatcac ttattgttgg
    39181 tattggtacc ctctcaagaa gtgctcaaca agcagaggta gataaattaa aaagagaaac
    39241 tggaagtgat catgtatact ttaaagatat aaataaaaat caattagaat acataaagtc
    39301 tagaaaagat attaaaaatt taggtataac atctcactat ggctatacag atcctaatga
    39361 aagattagct ataaacctag aatatgcaaa taaaaattac ttgactaatc aatccaaact
    39421 tataaaaggt caccttccaa aagctagtaa tgaggtggtc gtagagaaat ggattttaaa
    39481 tagtctgggt ttaaaaccag aaataaatca gaacattact tttaaattat atcaaaaaga
    39541 aaaaccagag acatttaaag ttgtaggaat acttgaagat agacctattg agaaaaataa
    39601 agggacatgt gaaatttttt taaatctcaa tgaatcaaaa ttggataaat tttcttatgc
    39661 ttatattgaa tttaataatg gcattgatat aaatacaagc atagataata tagtaaaaaa
    39721 tactatgttg gatgagaata gtgttggaaa aaacaaaatg cttatagaat ctactaggga
    39781 aaatggtact ttagataact caagtaaata cactgcaatt actatgagcc tattttctgg
    39841 tattgtaata tatagtatct atgttatatc gatatatcaa agaatacagg aatatggtat
    39901 cttaagagct attggatcca ctaacttcaa gattttcaag tttatgtttt atgaactttt
    39961 catacttgct ttgatagcaa tacctatcgg tatatgcaca ggtattggtg gtgcacaaat
    40021 atttaatagg actgcaggaa atattcaatt tgaaggtaat gtaacagtta ccccttttgt
    40081 tattccagat aaaataatac ttttatcaat tggaagtata atactgacaa tactaattat
    40141 aagtttcttt acatatttaa agataagaag aatttctcct attgactcta taagaaaaac
    40201 ctttggaact gataaaaata taaataaggt caattctttg atatcaaaat taactttaaa
    40261 tatatcagta acaaaatcta tatctgctaa aaatatcttt aggaataaaa agggcttcat
    40321 tataattata ttatccatga gtctaggagg tataatgatc ataaaggaga attataaata
    40381 ttctttttca gatatacaaa ataaaaatgg tcaagaagaa acatatatga atgcagattt
    40441 tattctaaca aattttttct taaaactaaa tcaatctaat aaagctgatt ctttcaagga
    40501 tataaaagga cttaatgata atcagataga taaaataaaa aatataaatg gaattgacaa
    40561 agtcaaaact gccagcatct tagatacaag aatagaagta gataaattaa atggattaga
    40621 ttactacaat attattaatt caacgcctta ttataaggat tttcctctat tcataaaaaa
    40681 taagaatact ggaaaatata caatgaaaca aaagttaaga ggttacaatg atgagatgat
    40741 aaattcatta gaaaaatact tagtgagtgg gagtataaat ctggaaagaa tgaaaaaaga
    40801 aaacctcgct atattatacg taccacaagt ttctaaaaca aataaataca agctaagtta
    40861 tactcctggt acagaaacgc ctgctgtaga tataaaagta ggagatacta taaaggtcaa
    40921 atatccaaaa ggtgagatag atcaagatct ttatataaaa ctaaaagata attatgaata
    40981 tctagaatat gagtttaaag ttggagctat tgtaagttat ccttttgcag ataattacct
    41041 atattctgga gatcagggta tagatgtaat aacaagtaac gactatctaa aaaaactaac
    41101 tggtgtagat aaatataatg tagtttatgt agatattaat aaaaatgcaa atgtaaaaaa
    41161 gataaataca ctcttaggtg aaataggaag tgaatctcct ggaactacaa cagctaatat
    41221 gctaatggaa aaagaaaact ttgataagat gacagctagg gctctgactt atgcttatgg
    41281 aatagtagca gtaatgttta taattagtgt gtttaatata ataaataaca taagttataa
    41341 cttaacttca agaactagcg aatttggaat gttaagagct attggtatct ctgagagagg
    41401 atttaaaaat atgatactct atgagggaat attatatgga atactctcaa gtgtaattac
    41461 cattgtggta gggcttataa tacagtttag aatgtattac acgtataatt ttgtatcata
    41521 tggattggga ttttcaatag attacaaaat atatatccta gttgttttag caaacataat
    41581 tgtaggtatt ctagctacat atattccatt aaggaaaata aataaaatta gtatagttga
    41641 agctataaac attacagaat aggaggatta attatgccta tattagagat tcttaactta
    41701 ggtaagattt atggaaaaaa ggaaacagct gtacatgcac taaaggatgc aaatttaaaa
    41761 ataaataagg gtgaatttgt tgcgataatt ggcccaagtg gaagtggaaa aagcacattt
    41821 ctacatttag taggaggtct tgagagacca agtagtggaa ctataaaagt cgatggtaaa
    41881 gatatatgtt gcctttcaga taaaaaatta gcaaaatata gaagacaaaa agtaggcttt
    41941 gtttttcaac agtataacct aattccagta ttaaatgtaa aagaaaatat agaattacca
    42001 ctaaagttag acaataaaaa agttgatgaa gaatacatcg aagacattat aaatttattg
    42061 ggtcttagtg agaggaaaaa tcatcttcca aatcaattat ctggtggtca acaacaaagg
    42121 gttgctattg cacgcgcttt atctgcaaaa ccaagtataa tcctagcaga tgaaccaaca
    42181 ggaaatttgg atagtaaaac tacggaagaa gtcatggatt tactaaaaag ttctataaaa
    42241 aaatataacc aaacacttat aataataaca cataatgaaa atattgctag aaaagcagat
    42301 agaattatat ctataataga tggagagctt aaattaacac tctaaaacta tttatagaaa
    42361 agaggattta aatgttattg attttagata atttaaattt aaacttagat tttgactaca
    42421 gaataataag agaagaaaat gatgatgtag atatatttat agatattaat tatcgtagct
    42481 tagatataga tactgatggt tctaacttat ttaattctag aatacaattt ccatttgtaa
    42541 gagcattgat tctaaggata aataagaaca atcaatgtat gacaattcat ttgttaagag
    42601 atatagattt attctctgct tttgccaact ttgaagtgga ttatactgac tctataataa
    42661 atataaaaaa tcaaaatgag aaagtaattt taaataaatc tatcaaaaaa tagtaagggg
    42721 gccttaatag gccccaattt ttttcttttc taaatttatt atatcatgat aaataaatta
    42781 gtcaatagaa ttttctgaat atttttataa ttttttcata aagtttttta tatattatgc
    42841 tttaaaatta tatgtaataa aaaaattttc cgactaattt taaagttaat tgattaaata
    42901 cacatattcg aaattaccat taaattactg actttttatc atttccattc agaatttaag
    42961 ttataatcta ttatttatag ttacttctac gtttctctaa taaaactcta tttatattca
    43021 aatctaataa tttatataca aacccaatag aacctatgtt gtacaatgaa tattatttac
    43081 cataataaac ttttagatat atctctttta attcactcat caatgggtat ctaggattag
    43141 cacctgtaca ttggtcatca aaagcttgtt caaccataac atccaatgtt tctaagaatt
    43201 tagattcttc tactccagcc tcttttatag ttttaggaag tcctactctt gcctttaatt
    43261 catctattgc ttttataagg ttatctactt tttcttggtc tgtatttccc ttgattcctg
    43321 caaaagaagc aaattcagca tatctttgta tacaatcagg atatctgtat tgtgagaatg
    43381 cacccatctt atttggagct tctgcacaat tgaacttaat tacttcattt attaataatg
    43441 catttgcaac accgtgttga acatggtgga atgcacctaa tttatgtgct aaagagtgac
    43501 atattcctaa gaaggcattt gcaaaagaca ttcctgccat agtagatgcc attgccattt
    43561 tttctcttgc ttctttatca ttctttccat ttttgtatgc acgaggtaga tattcaaata
    43621 ttattttacc tgcttgtagt gctagaccgt cagctggttc agttcttaac atagatacat
    43681 atgcttctaa tgcatgtgtt aaagcatcaa cacctgatgc tgctgttaat cttggtggca
    43741 tctccatcat catatcagca tcaattatag ccatgtttgg cattaattca taatctgcta
    43801 atgggtactt tacacctgaa tcttggtcag ttataactgc aaatggagtt acttctgaac
    43861 cagttcctgc tgaagttggg atagcagcaa aatacgcttt ttcacccatc ttaggaaata
    43921 catatactct ttttcttata tccataaatc tcatagccaa atcttggaag tctacctctg
    43981 gatgctcata cattacccac attatcttac cagcatccat tgcgctacct ccacctacag
    44041 ctattattaa atctggttgg aaatcagcca tagccttagc tccttctctt gcacaagcta
    44101 atgttgggtc tggagcaaca tcaaagaatg tagtatgctt aattcccatc tcatccaaca
    44161 aatcagttag cttcttagtg tatccattat tgtaaaggaa agaatctgtt actataaagg
    44221 ctttcttttt atccataaca tctttaaatt ctcttgctgc tacacctaaa caaccttttt
    44281 tgaaataaac cttttctggt gctctaaacc aaagcatatt ctctctcctt tcagctactg
    44341 tcttgatatt aagtaaatgt ttcactccca cgttttcaga tactgagttt ccaccccaag
    44401 aaccacaacc aagagtaaga gatggtgcta atttaaagtt atataagtca cctataccac
    44461 cttgtgacga tggagtatta actaatactc tacaagtttt catagcattt acaaacttct
    44521 ctactctatc tggatgattt atagagtctg catataacga agatgtatgg ccatatccac
    44581 catctgcaac taatttttca gctttagcaa ctgcatcttc aaaactttta gatttgtaca
    44641 ttgcaagtac aggagaaagt ttttcatgtg caaaaggttc atttaattca acagattcta
    44701 cttcaccaat tattattcta gctgtttttg gaacttctac atccgccatt ttggctatta
    44761 catgtgctgg ttgtccaact attctggcat ttaatgaacc atcaattatt actacttttc
    44821 ctactttttc agtttcttct ttatttagga aatgtgctcc acggtattta aattcttttt
    44881 taacttcttc atatattttt tctggtacta ttacagcttg ctcggaagca catatcatac
    44941 cattatcgaa tgattttgat actaagattg aacttacagc catttttata tctgcactct
    45001 catctattat tgcaggtaca tttccaggcc caactccaac agctggttta cctgaagagt
    45061 atgcagattt aaccattcca ggtccaccag tagctaatat aaggtctgcc tctgccatga
    45121 caacattagt taattcaatc gaaggaacat ctacccaacc tataagtcct tttggtgctc
    45181 ctgccttaac tgccgcatca tgtacaactc tagctgcttc tatagtacag tttttagctc
    45241 ttggatgagg agagaaaatt ataccatttc tagtttttag tgctattagg gatttgaata
    45301 ttgcagttga tgtaggatta gttgttggta taactgctga taaaacacct actggctcag
    45361 caattcttgt aaaaccaaat gcttcatctt tttctatgac accacaagtt ttagtatctt
    45421 tataagcatt atagatatat tctgaggcaa aatggttttt tataacctta tcttcaacaa
    45481 tacccatacc agtctcttcc actgccattt ttgctagagg tattctttgt tgattagctg
    45541 cagttgaagc agctaagaaa atcttatcta cctgttcttg cgtaaatgtt gcaaattctt
    45601 tttgtgccaa tctaatttct tctaatctca ttcttaatgt ttcaacactg ttgacaattt
    45661 caggactaca tacttcaaca tttgattttt ctactacctt ttcttttttc tccatactaa
    45721 aaacctccct aaaataatta tgcatctcta atttttataa atcttaaatg caccatctat
    45781 atttttaact tatacttaaa ataattttat agatttcatc tctatacctt agattttatt
    45841 tattttactc tcttaggtca agaagttttt ttctgaatat tttgtcaatt tattttttaa
    45901 caaatatatt atttttttta cttttactaa aattttttat ctaaa
//
LOCUS       DAMKPG010000022        45706 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMKPG010000022 DAMKPG010000000
VERSION     DAMKPG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45706
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.024"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..108)
                     /locus_tag="PC621_001308"
     CDS             complement(<1..108)
                     /locus_tag="PC621_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF4108475.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
     gene            complement(196..1290)
                     /locus_tag="PC621_001309"
     CDS             complement(196..1290)
                     /locus_tag="PC621_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF4108476.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(1292..1738)
                     /locus_tag="PC621_001310"
     CDS             complement(1292..1738)
                     /locus_tag="PC621_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4108477.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            2044..3234
                     /locus_tag="PC621_001311"
     CDS             2044..3234
                     /locus_tag="PC621_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4108478.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            3404..4366
                     /locus_tag="PC621_001312"
     CDS             3404..4366
                     /locus_tag="PC621_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF4108479.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(4484..5146)
                     /gene="zmp1"
                     /locus_tag="PC621_001313"
     CDS             complement(4484..5146)
                     /gene="zmp1"
                     /locus_tag="PC621_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF4108480.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(5246..5331)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5517..8372)
                     /locus_tag="PC621_001314"
                     /pseudo
     CDS             complement(5517..8372)
                     /locus_tag="PC621_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
     gene            8438..8514
                     /locus_tag="PC621_001315"
     tRNA            8438..8514
                     /locus_tag="PC621_001315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8472..8474,aa:Arg,seq:cct)
     gene            complement(8723..10168)
                     /locus_tag="PC621_001316"
     CDS             complement(8723..10168)
                     /locus_tag="PC621_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HDF4108481.1"
                     /translation="MASIKQRKSSFSVIYWYLDSAGERKQKWDTLETRKEAKQRKAFI
                     EYYQEKFGYVIVPLEEQFAHQIENSKKELDVVDKDITLRDFLEIFVNLYGTSKWSPST
                     FSSKVGSIENYINPIIGDWKLNEITTKSLSKYYNDLLSVPEVPRANRKATGRCVQPAN
                     IKKIHDIIRCALNQAIRWEYIDTNKRNPASLATLPKIPKTRRKVWSVQTFREAVKATE
                     DDLLSICMHLAFSCSMRIGEITGLTWEDVIIDEESIATNNAKVIINKELSRVNAEAMQ
                     KLKEKDILKIFPTQKPHCTTRLVLKTPKTETSNRVVWLPKTVAELLVQYKKDQQELKE
                     FLGSAYNDYNLVIALDNGNPVESRIVRDRFQKLCEENDYEVVVFHSLRHLSTGYKLKM
                     TNGDVKSVQGDTGHAEAEMVTDVYSEIIDEDRRFNAQKMDKEFYSTLNDDTDNPKQDT
                     DLTDSDMALLELIKSLSPEMKEQFLKQALQR"
     gene            complement(10187..10588)
                     /locus_tag="PC621_001317"
     CDS             complement(10187..10588)
                     /locus_tag="PC621_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108482.1"
                     /translation="MIRPHGAVLPTRMGIVEILTELSKAVCLLIIDSFSNKIYLLFLF
                     RCVIMTLLDECILLLSQMKGVLIVISDTQNAQSPTKEKKTYTVEEIAEQLDISKKVAY
                     SLVKSGQFSYVRAGKAIRVSKVSFDKWLNEI"
     gene            complement(10839..11087)
                     /locus_tag="PC621_001318"
     CDS             complement(10839..11087)
                     /locus_tag="PC621_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108483.1"
                     /translation="MKHTMKKPSYYLLSQAMDGDEKAIEKILAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(11094..11510)
                     /locus_tag="PC621_001319"
     CDS             complement(11094..11510)
                     /locus_tag="PC621_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4108484.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQKKLKRRQKGETLFC
                     ELPEIVVENLAVWDDYDTDYTIFNVCGSDIRICDDELAEALKHLSERNRENLLMYYFL
                     EMSDTEIAKRQNISRSGVFQNRHNSLELMKKILKEK"
     gene            complement(11958..12836)
                     /locus_tag="PC621_001320"
     CDS             complement(11958..12836)
                     /locus_tag="PC621_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF4108485.1"
                     /translation="MIQYYYTKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSTTIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLAYSMQ"
     gene            complement(12939..13223)
                     /locus_tag="PC621_001321"
     CDS             complement(12939..13223)
                     /locus_tag="PC621_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDF4108486.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
     gene            complement(13369..14106)
                     /gene="erm(B)"
                     /locus_tag="PC621_001322"
     CDS             complement(13369..14106)
                     /gene="erm(B)"
                     /locus_tag="PC621_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDF4108487.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(14231..14572)
                     /locus_tag="PC621_001323"
                     /pseudo
     CDS             complement(14231..14572)
                     /locus_tag="PC621_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(14633..14782)
                     /locus_tag="PC621_001324"
                     /pseudo
     CDS             complement(14633..14782)
                     /locus_tag="PC621_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(14784..15056)
                     /locus_tag="PC621_001325"
     CDS             complement(14784..15056)
                     /locus_tag="PC621_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDF4108488.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(15073..15288)
                     /locus_tag="PC621_001326"
     CDS             complement(15073..15288)
                     /locus_tag="PC621_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDF4108489.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            15475..15834
                     /locus_tag="PC621_001327"
     CDS             15475..15834
                     /locus_tag="PC621_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108490.1"
                     /translation="MRKAKETQTFDFRPLGLAIREARERAGLSRNDLGDKVFYGERHI
                     ADIENVGSHPSFQLFHDLVTMFNISVDEYFYPAENVVKSTARRQIETSLDLLSDNELK
                     IIQGTIDGILNSRESKK"
     gene            complement(15942..16130)
                     /locus_tag="PC621_001328"
     CDS             complement(15942..16130)
                     /locus_tag="PC621_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDF4108491.1"
                     /translation="MDKQLWVLCPICNNKTRTKVREDTELINFPLFCPKCKTETLINI
                     KHGKVTIIKEPDAKTQSR"
     gene            complement(16335..16526)
                     /locus_tag="PC621_001329"
     CDS             complement(16335..16526)
                     /locus_tag="PC621_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HDF4108492.1"
                     /translation="MRKCLRCHEEMVENLDVKVDMQGYGIRITTKGVFGTTVEKPKVA
                     VCPKCGEVSLYIENFKSIK"
     gene            complement(16539..17183)
                     /locus_tag="PC621_001330"
     CDS             complement(16539..17183)
                     /locus_tag="PC621_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF4108493.1"
                     /translation="MKGLLMKDLLVIQKKYGITRIIMDIFIIVALMLFLKGTGAIYIS
                     LLLIPVEIMTMIISLTTCDEQWKWGKYVVALPVSKTTIVKSRYIVALIMTCIGFGVAL
                     LVNTITYFIFPQFAFGYYMFIACASFAVTLVFLSFVLPSNYSLGVNAGFAVMFTLVII
                     LVVLAVWTNVTNNSILWFVVDNFELSIGIAFIIVIALAFSSYLLSKAFFTRKYN"
     gene            complement(17180..18043)
                     /locus_tag="PC621_001331"
     CDS             complement(17180..18043)
                     /locus_tag="PC621_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108494.1"
                     /translation="MAENILEVKNLTKDYGDFVLNDLSFSIPRGVIMGLIGENGAGKS
                     TTVNCILNEIKKNSGNILIFGKDHIIDECEIKDKIGIVFDENHFPDIFTPKEIEKYMS
                     GIYTNWDSIAFRNYLKQFELPAKKKIKDFSKGMKVKLAFAVALSHKAELLILDEATSG
                     LDPIIRDDILDILIDFVQDENYSILFSSHITSDLEKIADYITFIHKGRLVFCSLKDDL
                     IDNYGVVSCGASVFDTLDKTEIISYQKEDYQYKVLVRDRNKIAKDFPKALVEPATIEN
                     IMLFYVKGELQ"
     gene            complement(18044..18421)
                     /locus_tag="PC621_001332"
     CDS             complement(18044..18421)
                     /locus_tag="PC621_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4108495.1"
                     /translation="MEIFINNHASIPIYEQISSQIKALIINGELKAGEALPSIRGLAK
                     SLHISVLTVQKSYDVLQKDGFIETTAGKGCFVSAQNQDFYIEEQQKKIEDRFVEAIEI
                     ARASGINLDKLIGLLKLLYEEDE"
     gene            complement(18542..19453)
                     /locus_tag="PC621_001333"
     CDS             complement(18542..19453)
                     /locus_tag="PC621_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4108496.1"
                     /translation="MFKKNKKQTETIKEPKEKKVRTVKVGTHKKTVIALWVVLIASVS
                     FGVYKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQA
                     ISGYLTKELQDLNIDTIRTDIPTSSTVTDVIVWHIEQSGTDTFSATYEVDQQIKEGEQ
                     TSNVKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYDPKTPETDASVDADTANDATA
                     FLETFFKLYPTATENELAYYVSGNVLEPIGRDYLYSELVNPIFTKDGENVKVKVAVKF
                     LDNQTKATQVSQYELVLHKDSNWKIVG"
     gene            complement(19469..20476)
                     /locus_tag="PC621_001334"
     CDS             complement(19469..20476)
                     /locus_tag="PC621_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF4108497.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAREYGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGKGNKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYSYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGFADLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(20473..22668)
                     /locus_tag="PC621_001335"
     CDS             complement(20473..22668)
                     /locus_tag="PC621_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF4108498.1"
                     /translation="MKERIKGAFTKKKIFHFLKMVLFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTIAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSKGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYLAHRARRMERRLAGAVTAGGVAGAVAGSTISNRKATRNTATKDSRGNTSSSMGQR
                     VGSKVGAVLDTKNKVKDKASAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGMVQE
                     QQSRQSGRMEKREQHRQNIADKRMELQKAQEARQAQRKADGSATTGATRPHERPATAS
                     TIPKPSAEKTQEVKRPATATTSKTNEPVKTTVIKERPLSSGTSDRKTSQPTQPVHRQN
                     VEKVVSQETRQNDTKDRRTKVQQTQSVQKNQQTTEKTRNLVMKKGQKKK"
     gene            complement(22668..25118)
                     /locus_tag="PC621_001336"
     CDS             complement(22668..25118)
                     /locus_tag="PC621_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108499.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIHSMQEQSKKLVTGKLKEVACQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFTEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRNGIAYEDYEYQLPKKKLNKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVVRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKGNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSNSRGLLHVIDELRREDTPISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(25096..25494)
                     /locus_tag="PC621_001337"
     CDS             complement(25096..25494)
                     /locus_tag="PC621_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4108500.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDIPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQTLNETITAVRSVNYVPDKIY"
     gene            complement(25612..26115)
                     /locus_tag="PC621_001338"
     CDS             complement(25612..26115)
                     /locus_tag="PC621_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF4108501.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIAIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GICEIPY"
     gene            complement(26133..26636)
                     /locus_tag="PC621_001339"
     CDS             complement(26133..26636)
                     /locus_tag="PC621_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF4108502.1"
                     /translation="MQETRVLVETRETTGEETTSYWFDLPIDVAEFEEKLGVGAESTA
                     YRIIEKVLPYADEVHEHTSVYQLNELEFMYRQLSSDMQEEYVSLLTVFENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDSTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(26555..26851)
                     /locus_tag="PC621_001340"
     CDS             complement(26555..26851)
                     /locus_tag="PC621_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF4108503.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWREMKRLSPADYEERQL
                     EDFSKFVFGMPYQTLKDVMKQQKGREEQCRKQGCWWKREKQLAKKQHHTGSTCR"
     gene            complement(26936..27712)
                     /locus_tag="PC621_001341"
     CDS             complement(26936..27712)
                     /locus_tag="PC621_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108504.1"
                     /translation="MSLGEKIFKLRKEKGISQEALAEQIGTTRQAISKWENNQGFPET
                     EKLLQLSNIFEVSTDYLLKDEKNVKNSEERGYYVSKEMSVGFIANQKKLGLYLALGVM
                     LCIFAGIPYTLFLDNMTWRYLGMAFCFVAGIIFFVVAIFYEKQEYNILRQEPLLLDYE
                     YLKELKSEYTSKKKKYVIVAVPCTVLFIAGLIFFLFTVKGYISWTEYHSFAFFGLAVG
                     MLGFVYTINVMETYELLVKNEQYSTSFFFKLKRKIKKRLG"
     gene            complement(27916..28137)
                     /locus_tag="PC621_001342"
     CDS             complement(27916..28137)
                     /locus_tag="PC621_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108505.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(28138..28272)
                     /locus_tag="PC621_001343"
     CDS             complement(28138..28272)
                     /locus_tag="PC621_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF4108506.1"
                     /translation="MWVLLKDFLLVSMGMGIGIVLMCILNVGKEADYKMKQLEESEDN
                     "
     gene            complement(28285..29481)
                     /locus_tag="PC621_001344"
     CDS             complement(28285..29481)
                     /locus_tag="PC621_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF4108507.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHSEDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEQQAKLTEKHEMIIKQQTTPA
                     KDLVEI"
     gene            complement(29665..31059)
                     /locus_tag="PC621_001345"
     CDS             complement(29665..31059)
                     /locus_tag="PC621_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108508.1"
                     /translation="MRKIWNRGHRIRASDKHLVYHFSIGTLLFLFVAVLLLLNIKQLM
                     RTDWEHFSLLENGLTLSPYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLKLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMENVY
                     HTKEKMIDCVNAFYEGMVQRSEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(31160..31486)
                     /locus_tag="PC621_001346"
     CDS             complement(31160..31486)
                     /locus_tag="PC621_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108509.1"
                     /translation="MNLNIVLAVICGAVALVGAFCVVFQIYHMTVIDATARGLKHPKF
                     WGVFTMSGNNSSGLLMYLIGRRKYPIVNMSESNSKELEKRKKSAGIGLLFLAIGVIGI
                     ICATLI"
     gene            complement(31502..31909)
                     /locus_tag="PC621_001347"
     CDS             complement(31502..31909)
                     /locus_tag="PC621_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108510.1"
                     /translation="MEIGKKLKNARVQSGMTQENVAEKINVSRQTISNWENEKSYPDI
                     INVIELSNLYSISLDELLKGDEKMIEHLEESTNVVKSNQKMIWAIIVNIIVVALLIAL
                     NMFIPDNRYFLVGIFCLMVITSSALLYQIIKKF"
     gene            complement(32077..32460)
                     /locus_tag="PC621_001348"
     CDS             complement(32077..32460)
                     /locus_tag="PC621_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF4108511.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFMQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTFGGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHVPQGQTKK"
     gene            complement(32476..32802)
                     /locus_tag="PC621_001349"
     CDS             complement(32476..32802)
                     /locus_tag="PC621_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF4108512.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKKFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(33035..36079)
                     /locus_tag="PC621_001350"
     CDS             complement(33035..36079)
                     /locus_tag="PC621_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF4108513.1"
                     /translation="MKKTLKRLCTGFLALATIVTALPTTPVHAESKQYWTESAERVGI
                     IEKVMNDGSIGSTFNEGMMKVEGKTAYCIDINTDFRNGYKTRADASSRMNANQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARTFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLKKTSSNASITDGNG
                     NYSIAGATYGVFTDKDCTKQLATLTTDENGNTDVAEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKIEAGKTATLNVSDTPKVTDTLIELFKIDMETKKDNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSYGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTIEETKAPNGYLLEGAYMQAGDKSEQIKGLYVTQITEDDDLAVLSGSNQFSVSDK
                     VIRGGVKIQKRDLETGDMKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFTITENGKIVDLTDKAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHIVVTDNNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYVIEELRSESNKGFELIPPFEIVVSRN
                     NLLIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEITIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRAENDYTFVADEEEMKVEIAYTFNASALGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATNKDGNKELEAGKNVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKCVENDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPQIPEKPS
                     RPSDSPKTGDSTNVMAFLVMLLASAGGLAGTYLYKRRKMKKS"
     regulatory      complement(36598..36683)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(36809..37987)
                     /locus_tag="PC621_001351"
     CDS             complement(36809..37987)
                     /locus_tag="PC621_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF4108514.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            38359..38544
                     /locus_tag="PC621_001352"
     CDS             38359..38544
                     /locus_tag="PC621_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF4108515.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(38609..41383)
                     /locus_tag="PC621_001353"
     CDS             complement(38609..41383)
                     /locus_tag="PC621_001353"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF4108516.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(41555..42811)
                     /locus_tag="PC621_001354"
     CDS             complement(41555..42811)
                     /locus_tag="PC621_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF4108517.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(42883..44385)
                     /locus_tag="PC621_001355"
     CDS             complement(42883..44385)
                     /locus_tag="PC621_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF4108518.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(44476..45666)
                     /locus_tag="PC621_001356"
     CDS             complement(44476..45666)
                     /locus_tag="PC621_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108519.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
BASE COUNT        13260 a         9190 c         6932 g        16324 t
ORIGIN      
        1 ttcatttaca tctttaatgt ctttttcttc ttgaatttca tcatatatag cataagcaat
       61 ctttttagta tttccagtta aacttgaata taaaactaaa cttttcatga tgtctcctcc
      121 tctttatcta aatacttatc taaatcacca aaaatatatt aaccttaaca aattatttat
      181 ttttataaag atattctaat ctagtataca tccaaactga gagcgtgttt gtaaagcatc
      241 tgcacacttc ttattacaat aacagcactt tttaatctca tctactcttc catcttttac
      301 tttctgtaac caattactat ctgctagtaa ttgtcttgac atagaaacca tatctactct
      361 attatttgaa ataatacttt ctataaaatc tgggctagac aattttccaa ccccacatac
      421 tggaagattt gtgtgtttct ttacttcatc agctaaatat aaaaaacagc cttcatcttt
      481 aaagtaagga tgattattgg ttggtatagt atcttctaat tttgaatgat ttgcaagtgt
      541 tacatgaaaa ctgtttacac ctagggattc aaatttttta acaaaatact caacttccga
      601 aagtaaaact cctgcatttc cataatgagg attttcttgt cttattgcaa atttatagtc
      661 tattggaatg tccttaactt catctcggat actactaatt atttcgcttg caaatctact
      721 tctattatca cacgttccac catattcatc atttctatta ttaaatatac ttgatgaaaa
      781 acttcctaca agcctatcac catgaatttg aatcatgtca aaacctgctt tttttgcatt
      841 caatgctgtt actttaaata aatttataat atttttaatt tcttctttgg gcatatttgt
      901 tatgtaatct tttactccgt cattcattat tttttttatc tcatcatgtg aagtaattcc
      961 catcttcata gtatctttaa ttaaattaac atcataatct gaacaaaata gttgtgctga
     1021 tacctttgca ccagaattgt gtatttcatc tacaattttc ttgtatttaa tgactccatc
     1081 ttcatccgat aggcttatag ctaccttatg aaaactaggt ctaatagata catctcctag
     1141 agtaatcaga gctactcctg acttagcaat atcagaaaac tttttaattt tttcttccaa
     1201 tttaagaccc atgcttgtag gtgcaaaaga tactctattt tttaattcca aatttccaat
     1261 tttaattggt tttaataaat tacaatacat cctatccttc ctccacagct gtttttatat
     1321 cctttaccac atgtaaattc tttaccagtt ctttatattc tgtatctgag aactcttttt
     1381 ttatgtggtt atcccacttt gaaaatatgt tttttatatc ccctaatact tcctcaccct
     1441 tatctgttac tctcaactga aaagctctac tatcattagg gttttggatt ttttctatgt
     1501 atccatcttg ttctaaccta gatatagttc ttgttgtatg agctttatca acttcaaatt
     1561 ctgtagaaac atcattcaaa gaacaattcc tatttttatt aatataaatt aaatagaaat
     1621 atagaccatt cgtaagttta tatggtttta aacatttttc acaataaagt ttaaagtttt
     1681 tttgcaagtt acaaataata tatgacaaac tttcatactg taaattttta aaatccatct
     1741 taaatttccc ttctataatt ataatatgtt tgactatatc aactatatta tattaaacaa
     1801 ttaatatagt caagaatatt ttattctcta aatctatgtt ttattaattc tttaaaaaac
     1861 tattatataa aataatcatg ataaaattag aaattggaat ttttaatatg cactgtacat
     1921 aaatatattt tatataaaag tttatatttt caaacaagta atttttcgaa aattgtctaa
     1981 attcagtaaa aatatggtaa tatatacata agcaatataa atctataaaa aggagtgtta
     2041 catatgatat ctaataaaat gcaaacttta gttgcaaata gttctgttat aagagctatg
     2101 tttgaagaag gaaagaaact ttctgatatt tatggtgagg aaaatgtttt tgattttagt
     2161 ataggaaatc caagtgttga acctccagag actattaaag cagttataaa tgatatttta
     2221 aatgaagaat ctccaaattt agttcatgga tatatgaaca actcaggata tgaggatgta
     2281 agagatgcta ttgctgaaca tataaataaa aaagatggat taaatcttac aagagaaaat
     2341 ttaattatga cttgtggtgc tgctggtggt ctaaatatta ttttaaaaac tcttttaaat
     2401 cctggtgatg aagttattgc ttttgcaccg tattttggtg agtataaaaa ttatacagaa
     2461 aactatgatg gaaaattaat agaagttcct acaaatatag agacttttga accagattta
     2521 gatgctctaa aaaatgctat tactccaaaa acaagagctt taataataaa cactccaaat
     2581 aatcctactg gtgtcatata ttctgaagaa ttattaaaaa atcttggaga attattggat
     2641 tcaaaacaaa aagaatttaa tacaagcata tatctaattt ctgatgagcc atatagagaa
     2701 ataatttatg atggtgccaa agttccttgt gtactaaaat actatagaaa ttcatttata
     2761 gggtactctt atagtaaatc attatctcta cctggtgaac gtataggata tattgttgct
     2821 aatgggcaaa tggatgattt tgatgatgtt atgtcttcat taaatgttgc aaatagaata
     2881 cttggatttg taaatgctcc ttctcttttt caaagggtta ttgcaagaag tttagatgca
     2941 gaagttgatg taaatattta taaaaagaat tttgaccttc tatataatag tttaatcgat
     3001 atgggatatt cttgtgttaa acctaatggt acattctatt tgttcccaaa agctccaatt
     3061 gaagatgata aaaaattctg caatgatgca aaacaattta atttattatt agtaccaggt
     3121 tcttcttttg gttgtccagg tcattttaga gtatcatatt gtgtatctta tgataaagtt
     3181 aaaagttctt taccagcatt tgaaaaatta gccaaattat ataatttaaa ataactaaat
     3241 caaagtaatt caaataatta actaccttag tatagaatac ttacattcta tattaaggta
     3301 gtttataatt ttatgtacta tattttatac ataccatatt attccaatat gcttgaatat
     3361 tcttatctat agtactaata taaattacaa ggaggtgttt gacttgaaaa aaggttatgt
     3421 ttatataata ctgactacct tattttttag ttcaatggaa attagtctaa aaactgtcac
     3481 taatgacttt aatcccttac aaataacttt atcaagattt tttgttggcg gtttagtctt
     3541 actacctttt gctgtaaaaa gattaagagc tcttagtctt tctattacaa aatcagactt
     3601 aaaatacttt gcatttttag gttttatgtg tgtagtagta agtatgtcat tgtatcaact
     3661 ggctgttcta aacacaaagg catctgtagt tgctgttttg ttcagttgta atccagtctt
     3721 tgtaatgctt ttagcttact taattttgaa ggaaaaaata tataggcaca atgtaatttc
     3781 acttatatta gaagttcttg gaattatagt tataataaat ccattgcata caaaactaac
     3841 tcttagtgga atattcttaa cattatcttc tgctattatt ttttcaatgt atactgtatt
     3901 tggtaaaaga aaaaccttaa aatttggtgg aatagttgta acttgcttta gctttatatt
     3961 tggtagtttg gaaatgttga tattggtttt actcactaaa attaatttta tcgcagatat
     4021 attaaataaa aataaacttc atatatttgc aaatatccct atttttagtg gatatacctt
     4081 acacaatatg cccattatga tttatgtatt cgtatttgtt acaggtgttg gttatgcact
     4141 ttactttatg gcaatggaag ctacatctac tagtcttaca tctttagtat tcttctttaa
     4201 accagtacta tctccaatac tagcattatt aatacttaaa gaaattatcc ctataaacat
     4261 ggttatagga attttattga tagtaattgg ttcaataatt tctattatac ctacgataat
     4321 tactcaaatg cataataaac acgttgaagc cttgtataag gagtagtaat tgataatcta
     4381 tcaagttata cataagacaa aatttattag atttaaacgc aatataaaag agatgctatt
     4441 ataatagcat ctcttttata tttattttaa atttattaaa gctctattta gctaggtttt
     4501 gcaaaaagct ataagtttgt ggacaagctg attttaactt actatttgta tcttgattta
     4561 aatagtaata tgcaaatgct tctgcaaaaa attcttctgg atatacacct aagtagttta
     4621 cattacctaa agaacgacct tctttagcaa atatttgttt gaattgtgct gatttagata
     4681 tatcatttaa tactatatgg tctattgcat gtgctgtttc atgtaattct aaatttattg
     4741 catcatgccc tttacctttg ttactaaatc ctattctaag agctactgta cttccaccta
     4801 agcctggaac atcatcccaa gtttttccag taccttccca tcccttagga actacacctt
     4861 ttaaataagc atattctttt tcatctgtta actttccact taataatttt atttttaagt
     4921 tatcttgctt taatacattt aaatactttc catctatatt agctaatcta tttaccatag
     4981 cattagcttc gtttttattg taatttccag ttggaaaaac tactatttga ctaagtgtgt
     5041 ctttattttg ttgtatagta gtactatatg catgtgcact tactggaact gaacttatta
     5101 agaatattga tattatagct attaataatt ttttacttgg tctcatatta ttccctcctt
     5161 tgagttaggc cgggagtaat aaatttcaaa ataaaaaaga tacccaagag tatctttgta
     5221 tatacaaaaa gaaattttta ctcttttcgg caactggctg gcatggttta ctaaacttta
     5281 gcccctatag ctttgcgtga ctaggtttcc ctagttttgc ctcatttaat tttatatcta
     5341 tattatagct tatatctttt taattgtcta tatccctttg gaactttttt ctaaaaagtt
     5401 attatttttt tacataaatc ttcaaaaaaa aatatcttct aacaatattt tcattattaa
     5461 atattgtttt agaagatatt ttttaatata acttatattt tttataatgt tagggtctaa
     5521 tttgtatttt tatttcttct taatacgaca agtcctacaa tagctcctat aactaaaagt
     5581 atttctccaa taattgtagt actatcccct gtttttggtg gtactggtgg atttaccaaa
     5641 gtatcatctt tagctttatt atttttttct tctgacactg atggctttac cacttctggt
     5701 attggaacaa tctgtttaac aggagtttct atcacctctt ctgatggtgg tactggtgga
     5761 ttcaatatct ctggtattgg aggatttatt atatcttctg acggtggtac tggtggattt
     5821 ataactatag tatcctctgt atcagatgga tttgatggtt tccttggttt atctgtgctt
     5881 ggtggcactg gtggatttac tattggttca tctgtatttg gcggtacagg tggatttact
     5941 ggtggatttt cttgtcttat gttttctata tttccgatat ctactattct accaatatct
     6001 ttattaccaa tttcaacctt aattttatcc ttcagttttt catatccact tggagccatc
     6061 acttctacta aataatatat tcctggtttt tttacactag attgaattac accatcttca
     6121 tcactaacta aatccttaac tactatattt ccaaatgcat cttctaaatt gaacttagca
     6181 cctgaaagta catttttatc taaagcatcc acttttttag cttcaaaagc ataaccttgc
     6241 ttagtagtct ctactcccgg aggacttgaa ccattaatct gagccacatt ttgtatagtc
     6301 tccccaactt ttacatttgt gaagtctgca tcaaatgtaa cccagctttg cacaggttcc
     6361 ccttctttta tctgtttaaa ttcgtattta tttactaatc ttattgtatt ttttccatct
     6421 ccagcttctt ttttaagttc aagattatca gaatatttta tattagatat ttttagagat
     6481 gggtttactt catccaaaag atttacttgt cctgcatcat aaaccccata agttgacata
     6541 ttgattttgt atttaacaat ctggttatca ctactattta aaaccttttt atcaacttct
     6601 ttttttgcgc ttattaatcc agaatttttc ttaaagttta tctcatcatt tttatcaatt
     6661 tcactattat ttgcatcaag gacagctcta tttatataac tatcttcaat actaattacc
     6721 tttaatgtat aagctataaa aaactttgat tctacttctt ctttactgag attaattgta
     6781 aatccagtta taactttatc tacttgccca ctttcatttc ttttttcttc tactttatat
     6841 gaaatattat tggcaatttt ttctttactc caaggagtcc agctcccatt cattggaact
     6901 tctacaactc taaaatcatg cccatcctct cctgaagtag tcaatctcat accttctggt
     6961 atattatctg ttataacaac attacttatt ggtgatttat catcatttat tgaaatctga
     7021 tagtttatta cttgaccaac ttgagtaata tttgttgctc ctgaatgtcc tttagtaaga
     7081 agataattat ctcctctatc aaatatactt acagtagcat catcttcttg tatcttctta
     7141 ccatcagatt caatcctagc tacattctta tatttattac catgttttgc atttttgtca
     7201 ctgattctag ccttatactt aacagtatac gatttatcta tatttccaaa attaactgaa
     7261 atagaatttt tactctgttc aatattaaaa tcactaactt cttttccatc acttgttact
     7321 acacttacag aaccatttat caatgtcaat ccttcaggaa gctcatcata caaaataata
     7381 tcttttaact cttcctgctt ttcatttact tgtatactgt aatttatatc tttattttta
     7441 actggcataa atacagtagt tgattttctt ccttcaacaa gctttttaag tgttccttta
     7501 gcatctggaa catccgtttt atctccaggt tttatttcta catctatatc tccacttcca
     7561 ctttctattt ttccagtggt ttcaatatgt atttttgcat tagtatcttc atcaaccttt
     7621 ttaacatatg cttccaaacc tattttacct tgcattacac tttggccatt aggtaattct
     7681 ctaaatgtaa gtgtatactc tccattactt tctgttttat caaagcattc acttgtataa
     7741 tctaaattaa ctttattgaa tacatctggc accttaaaag ttattgaatc tcctgcttgt
     7801 attccttttg atgttggagt ataagttaaa ttaaatgata tttttttcat tgattccgtt
     7861 tctattgtat tgttattaat ttttacaaca aatccatagt cttcattaag ttcttcttct
     7921 aaaactttca ataaattttc atcttctaat agattatctt cctctggagt tttttctgta
     7981 tttttattgt ctagtggttt ttcatcttct aaagcttgtt ctgacttttt attatctgtt
     8041 gacttatctt cttctggtat ttgctctggc tttttattat ctattgactt ttctccttct
     8101 ggagtttgct ctggcttttt attatctatt ggcttttctc cttctggagt ttgctctggc
     8161 tttttattat ctgttgactt atcttcttct gaagtctgtt ctggtttttt attatctgtt
     8221 gaattatctt cctctggggt ctgttctggt tttttattat ctgttgcctt atctttctct
     8281 ggagtctgtt ctggtttttt attgtcgctt tccttctcct ctccagttga tgaattagtt
     8341 gaaccacttt gaatctgact attctctcct aagggctagg tggaacaaac gccttttgat
     8401 atgataacta gataaaattg aatagatttt taagaatgtc ccagtacctc agcaggatag
     8461 agggttcgcc tcctaagcga aacgtcgcag gttcgactcc tgtctgggac gccacaaact
     8521 ccgctgaaag ccttatttta caaggttttc agcgtttctt tatttatggc tttctattta
     8581 cgctgaatac gaattgtttt caatctgtca ttttttattc gatatttttt taagactttt
     8641 aaggaaatag gcttgctaac acttttttat ttttgaagaa aaatcgctgg tttttttgga
     8701 atttgctaac acttttgaaa ttctatcttt gaagtgcctg ctttaaaaat tgctctttca
     8761 tttctggcga taaagattta atcagttcca ataacgccat atcactatcg gttaagtcgg
     8821 tatcttgctt tggattgtct gtatcatcat taagcgtaga ataaaattct ttatccattt
     8881 tttgtgcatt gaaacgtctg tcctcgtcaa tgatttctga atatacatct gtaaccattt
     8941 ctgcttctgc gtgaccagta tcgccttgta cggacttaac atctccgttt gtcattttga
     9001 gtttataacc cgtacttaaa tggcgtaggc tgtgaaatac cacaacttca taatcgtttt
     9061 cttcacagag cttttgaaac ctatctctaa caatacggct ttccacggga ttgccattat
     9121 ccaacgcaat aacaaggttg taatcattgt aggcacttcc taaaaattcc tttaattcct
     9181 gctggtcttt tttatattgc accaatagtt ccgcaactgt tttaggtaac cagacaacac
     9241 gattacttgt ttctgttttt ggcgttttca agacaagcct tgtggtacaa tgcggttttt
     9301 gcgttgggaa aatctttaaa atatcctttt cctttaattt ctgcatagct tctgcattta
     9361 ctcttgaaag ttctttattg atgattacct ttgcattatt tgtagcaatg gactcttcat
     9421 caataataac atcttcccat gtaagtcctg taatttctcc aattcgcata gagcaggaaa
     9481 aagctaagtg catacaaatt gaaagcaaat catcttccgt ggcttttacc gcttctcgaa
     9541 atgtttgaac actccacact tttcgcctag tctttggaat ttttggtaac gtagccaaag
     9601 acgcagggtt tcttttgttt gtatcaatat actcccagcg gatagcttga tttaaggcac
     9661 agcggataat atcatgtatt tttttgatgt tcgctggttg aacacaacga ccagttgctt
     9721 ttctgtttgc ccgtggtact tctggaacgc tcaataaatc attgtagtat tttgatagac
     9781 tttttgtagt tatttcattc agtttccaat ctccaataat cggattgata taattttcaa
     9841 tactgcctac cttactacta aatgtactcg gcgaccattt agatgtgcca tataaattaa
     9901 caaaaatttc taggaaatca cgcaatgtaa tatctttgtc tacaacatct aactcctttt
     9961 tagaattttc aatctgatgt gcaaattgtt cctctaatgg aactatcaca tatccaaatt
    10021 tctcttgata gtattctata aaggcttttc gctgttttgc ttcttttctt gtttccagcg
    10081 tgtcccattt ttgttttctt tctcctgcac tatctaagta ccaataaata actgaaaaac
    10141 ttgattttct ttgtttgatt gaagccataa ttttctctcc ttatagttaa atttcattta
    10201 accatttatc gaaagaaacc tttgataccc gtatggcttt accagcacga acataactga
    10261 attgaccgct ttttactaat gaataagcta cctttttgct aatgtccagt tgttcagcaa
    10321 tttcctctac tgtataagtc tttttttcct ttgtaggaga ttgtgcattt tgtgtatcac
    10381 ttatcactat taagacccct ttcatttgtg acagcaacaa aatacactcg tctaataagg
    10441 tcattataac acacctaaat aaaaagagta gatagatttt gttgctgaaa ctatcaatta
    10501 taagcaggca gacggctttg gaaagctccg ttagtatctc aactattccc attcttgtgg
    10561 gcagaaccgc accatgcgga cgtatcatta ttctgtgagg ataggtcatg gcaaaacgac
    10621 ttttccaaca gtcgctctcg gatcactgcg tgggtcgctc gctttctttt gaaaaggtcg
    10681 tggcgtacag ttcccgtggc tcgctatctc acaaacgtat ctgtctgctt tattcagttg
    10741 tcaaagagct gtggcgaaag cgtgttgctt tcatataaaa gcaggggcag agcaggaaag
    10801 aggggattga acaaatcatg ctctgcggtt actgcttgtt attcttcttc gatttctaag
    10861 gcaatatcaa accctaaaat cattttaata agtgcttctc tgataagtcc ctttaattcc
    10921 atatctacaa caatatagac attgccgtat tcatcataga gtggtcgtag acagcacttt
    10981 gatatgtaag ggtcataaaa tgccagtatc ttttcaatcg ctttttcgtc cccgtccatt
    11041 gcctgcgaaa gcaagtagta ggacggcttc ttcattgtgt gtttcatttg ctatcatttc
    11101 tcctttagta tttttttcat aagctctagt gaattgtgtc ggttttgaaa aacaccactt
    11161 cttgaaatat tttgtctttt tgcaatctcg gtatcgctca tttccaagaa ataatacatc
    11221 aacaaatttt ctcgattacg ctcagacaaa tgtttcaagg cttctgccag ttcatcatca
    11281 caaatacgaa tatcactacc acatacattg aaaatcgtat agtccgtatc atagtcgtcc
    11341 cagacagcca gattttctac aacgatttct ggaagttcac aaaaaagcgt ttctcccttt
    11401 tgtcgtcgct ttaatttctt ctgatagtcc ttaacaacat gtcggacaac tttcttcaaa
    11461 caactttcaa aacggcattg aactgttttc tggaagtcag atggtttcat caatctcacc
    11521 ccctttccgt tacgatatga aaagtggtta tccctctttc cgcaccatat agggaacgac
    11581 aggtgtgatt tgctaagttg aaaccgaaaa aagttgaaaa aaatttttaa caaaacaaaa
    11641 acagcccgat aatgtgtaat tacacaaaca gactgtttta taaattctat tgatatgaat
    11701 ttttaattct tgtaaagggt gtaatgattg taatagtgga ctgaaatatt tattcagttg
    11761 agaacggcag aacgttcttc cgtatgcaat catggtatag ccccctttaa ccagagctat
    11821 gccttattta ctcctaagaa aaaaggacag ttctggttat atccaaaacc gtcccgttat
    11881 ttttcaagac gcacaaaagt gtattatcta ggaacggttt tttttattta ccgcacacga
    11941 taacagcttt tgaattatta ctgcatacta tatgcaagca tttcaaaaaa tacgttctca
    12001 tacatagata aaactttttt gtcatagcct ttgtgttctg taatgccgtt tttagaccag
    12061 gtacttactt tattacttcg cttgataata ttttggaaaa ccaagttatc ttctttatgt
    12121 tgcttgtaca gttcttccag gtttgatttt atcgttgtgc tgtccgtatc aactaaataa
    12181 ggaacaaaac cgatcatatc tagtccaggg ttaaactgtt cttgtaaatc aatcaaatag
    12241 gaaatatagt tttgaatgtt gtttgtactt tcttcttctg cttgtaaagg gatcatgacg
    12301 taatcacttg ccacgattgc attatttgta taaacgcttg gcgttggtac agtatcaata
    12361 ataatgagat catagtcact ttttaaaggt gctaaaagag tagcaagcaa tctactttca
    12421 ttttcaaaag tccatgagcg agttaatttt ggcagtaaca tcaaatcaaa cgtgccaggg
    12481 atcaagtcta aattatcagt caaatgaaca atagaagaag ccaagtttcc atttttcagt
    12541 ccttcataaa aattaacacg tggcaattct accttaaatg tttttgctaa gtcttttgtc
    12601 aatgttgctt gtaagtcctt atcgatcatt aaaactttta aattaaattt gtctgtcaag
    12661 taagcaaaca tagtcgataa ttttgacttt ccaacaccac ctttaaaata attgtttaag
    12721 ataacaatcg cttcattttt actgtttaaa atacgtctta attcttcaag tattttgagt
    12781 tcttccttct ccataactac gccccattct ttttttgtat agtaatattg tatcattagg
    12841 aatcacaaat cacaagtgat ttgtgattaa tcacttgtga tttgtgataa gtgatttgtg
    12901 attaatatat aaaagccctc tttaaagggc ttttatgctt attttgaaaa agagataaaa
    12961 tcaatatatc ccttttctcc gatttttaca acggcattgt aggactttct atctttcgtt
    13021 ttgattcctt ttaccagggt ttcttttccc tctagtaatt cttttacatt tgttttggtg
    13081 agttttttct ttctaaaatg ttcagctaaa gtgaaggtac attcaggata atttgaacaa
    13141 ccataaaacg atttttttaa tacaatattg ttgccacact taggacattt tcctacaagg
    13201 ggtcccgagc gcttagtggg aatttgtacc ccttatcgat acaaattccc cgtaggcgct
    13261 agggacctct ttagctcctt ggaagctgtc agtagtatac ctaataattt atctacattc
    13321 cctttagtaa cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc
    13381 gttaaataat agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa
    13441 attgtttact ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg
    13501 atattctcga ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg
    13561 aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg
    13621 aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa
    13681 ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata
    13741 gatgtcagac gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta
    13801 aggaatactc ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc
    13861 ttggtgaatt aaagtgacac gagtattcag ttttaatttt tctgacgata agttgaatag
    13921 atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa
    13981 atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt
    14041 tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt
    14101 gttcatgtaa tcactccttc ttaattacaa atttttagca tctaatttaa cttcaattcc
    14161 tattatacaa aattttaaga tactgcacta tcaacacact cttaagtttg cttctaagtc
    14221 ttatttccat actcgctctt aatacaatat agtgtacctc gtaatcttct tgggcaagag
    14281 ctttccatgg ctccaaaaac catggcccga caatcccatc tacaattaca tcatatccgc
    14341 cacgagcata tcgctttgca gcctctaaaa aggcttcaat gacaaccaga ttttgcttgt
    14401 tggattctgg caaatgtggt ggtattgcgc ctttgcttaa gtaatgaaaa aagtcatcgg
    14461 tgtgcatatg cactgattta tccatatctg attcctttgc gacaattgac gcagttgttg
    14521 tttttcctgt ccccggtgag cctgtaatca caataattct tccttgattc atctatattt
    14581 tccctcacaa tttgaattta tcactttgtt cctgcctgct caatcatctt cttcgcaaac
    14641 tcgttttccc tgaccctggt tgcccaccaa gtaaaaaagc ggttggcgat tcaaccgctt
    14701 tttttccttg aaccaattct tctaaattat catttaagcg attttcaaat tgtttgtcag
    14761 taaaattgac tatatttgcc atattaagcc actttctctt tattcaaaac ttgttttgaa
    14821 tagcgattca ttgaccgcca atactcatga acggcttgta attcttctaa agaataatca
    14881 aaaagattta cacgttttgc aagctttaat tggtttaata aattgacttc caataattga
    14941 gagtcatttt tatttaattc atgattcaaa acatagttta atactcgatt ataaacgctt
    15001 gccgataatt cgttaatgat ctctatttca aatgtttttt cataagtaac tgccatttca
    15061 ttttcactcc ttttaaagtt tgtcaggtaa atatttccga atatattctt ctaaggcttt
    15121 atccataact tcttttacgt ttccgccatt ctttgctgtt tcgattttta taatatggtg
    15181 caagtcagca cgaacacgaa ccgtcttatc ccccataata tctttttttg cactgattgg
    15241 tgtatcattt cgttttgctt tttgtgcgcc taaatttccc acaaccactc acttctttct
    15301 atttcttctt actcttattt tatcatcaac aatcacaaat cacaagtgat ttgtgattaa
    15361 tcacttgtga tttgtgattc tattcttttt cgttttctaa ttgaatgatt cgctcaattt
    15421 taacacattc taaatataca gcatgaagta taaactcatg ctaggtgatt taaaatgaga
    15481 aaagcaaaag aaacacaaac ttttgatttc agacctttag gactggcaat cagagaagcc
    15541 cgtgagagag cagggctttc tcgtaatgat ttaggcgata aagtgttcta tggagaacgc
    15601 catatcgcag atattgaaaa tgttggttca caccctagtt ttcaattatt ccatgattta
    15661 gttaccatgt tcaatatatc ggtcgatgaa tatttctatc cagcagagaa cgtcgtaaaa
    15721 agtacagctc gccgtcagat agaaacctct cttgatttat tgagtgataa cgaattaaaa
    15781 atcattcaag gtacgattga cggtatcttg aacagcaggg agagcaagaa gtaatcagct
    15841 tcttgttttc cctttttgct attctgcatt atctatttgc tgatctgctc cgcaggtaac
    15901 aaataagagc caacaatcta taagtcataa taacctacaa attatcggct ctgcgtcttt
    15961 gcgtctggct ctttgataat agttactttt ccatgcttta tattgattaa agtttccgtt
    16021 ttgcacttcg gacagaacaa aggaaaattt atcagttctg tatcttctcg cactttagtt
    16081 cgtgtcttat tattgcaaat aggacataat acccataatt gcttatccat agccccccct
    16141 tattctttta agaaatttaa tttttgcttt ttcgtagtga tataaacata ttttttcggc
    16201 gtaaaatgtc tatttccaaa ataccttttg aaatttgctt gcatctctgg gtatgtacta
    16261 ttcataaatt cttcaatatc cgctcggacg ttagcaaaca ataaaccctc agtctatatg
    16321 cagattttaa tcatttattt tattgattta aaattttcaa tatataagga aacttcgcca
    16381 cattttggac aaactgcaac tttaggtttt tctaccgtag ttccaaaaac gcctttagta
    16441 gttattctaa taccatatcc ctgcatatca actttaacat ctaaattttc gaccatttct
    16501 tcatgacatc taagacattt tctcataaca atacctcact aattgtattt tctagtaaaa
    16561 aatgcttttg atagaagata agaactgaaa gcaagagcta taactattat aaaagctatg
    16621 ccgatactca attcaaaatt atcgactaca aaccataaaa tagaattgtt agtcacattt
    16681 gtccaaactg ccaatacaac aagaataatc actagagtaa acataaccgc aaaacctgca
    16741 ttaactccaa gtgaataatt tgaaggcaat acaaatgaca aaaatactaa ggttacggca
    16801 aaagaagcac aagcaataaa catataataa ccaaatgcaa attgagggaa tataaaatat
    16861 gttatggtat taacaagcaa agctacccca aagccaatac aagtcataat caaagcaact
    16921 atatatctgc tttttacaat cgtagttttt gaaacgggta gtgcaacaac atatttcccc
    16981 catttccatt gttcgtcgca agtcgttaag gaaattatca ttgtcattat ttcaactgga
    17041 attaacaata aactaatgta aatagctcct gtccctttta aaaataacat caaagctact
    17101 ataatgaata tatccataat aattctagta attccatatt ttttctgaat tactaataaa
    17161 tctttcatca ataagccttt cattgtaatt ccccctttac gtaaaacaac ataatatttt
    17221 caatagtagc aggttctaca agtgcttttg gaaaatcttt tgcaatcttg tttctatcac
    17281 gaacaagaac cttatattga taatcttctt tttgatagga aataatttct gtcttatcta
    17341 gcgtatcaaa gacacttgct ccacaactta caacaccata gttatcaatt aagtcgtctt
    17401 ttaatgagca gaaaactaac cgtcctttat gaataaaagt aatataatct gctatttttt
    17461 ctaaatcact tgtaatatgt gatgaaaaca aaatagagta attttcatct tgcacaaaat
    17521 caatcagtat atcaagaata tcatctcgaa taatagggtc caatccactt gttgcttcat
    17581 ctaatataag taattctgct ttatgtgaaa gagcaacagc gaatgcaagt tttactttca
    17641 ttcctttaga aaaatcttta attttcttct ttgctggtaa ttcgaactgt ttcaaataat
    17701 tacgaaatgc tatactgtcc caattggtat atattcccga catatatttt tcaatttctt
    17761 ttggtgtaaa aatatctgga aagtggtttt cgtcaaatac aattccgatt ttatctttta
    17821 tttcacattc atcaattatg tggtcttttc caaaaatcaa aatgtttcca ctgtttttct
    17881 taatttcgtt caaaatacaa tttactgtgg tcgattttcc agctccattt tcgccaataa
    17941 gccccataat tacacctcta ggaatggaaa aacttaagtc atttaataca aaatcgccat
    18001 aatcttttgt aaggttttta acttctaaaa tattttcagc catttactcg tcctcctcat
    18061 ataataattt taataatcca atcaatttat caaggtttat gccacttgca cgggctattt
    18121 caatagcttc tacaaatcta tcttcgattt ttttctgttg ttcttctata taaaaatctt
    18181 gattttgagc agatacaaaa caccctttgc ctgctgttgt ttcgataaaa ccgtcttttt
    18241 gaagcacatc ataagatttt tgaacagtca atacacttat atgcaatgat tttgcaagtc
    18301 ctcttattga tggtagagct tcgccagcct ttaattcccc atttataatc aatgctttta
    18361 tttgcgaaga aatttgttca taaataggta tgcttgcatg attattgata aagatttcca
    18421 tttaaacacc gccttgttct atactgtata gcttatacaa gtacagcata atacttaaaa
    18481 atacagttgt caatattaaa aggcttgcag agcataaaaa tatgatacaa gccttttaca
    18541 attatcctac aatcttccag ttactatcct tatgtagcac aagctcatac tgcgatactt
    18601 gcgttgcctt tgtctgatta tcaaggaatt ttacagcaac cttaactttt acattctccc
    18661 cgtcttttgt aaagataggg tttaccagtt cagaataaag gtagtccctg ccgataggtt
    18721 caagcacatt ccccgacaca taataagcaa gttcgttttc tgtggctgtt gggtacaact
    18781 taaagaatgt ttccagaaat gcggtagcgt catttgcagt atcagcgtct acacttgcgt
    18841 ctgtttctgg tgtctttgga tcatagtctg atttttcgat tgctggtgca agagtagggt
    18901 tctgaacgat taccatatcc ccgtcagcgt ctacatgaac ttttacagta taggttgctt
    18961 tcacattgct tgtctgttcg ccctctttta tctgctgatc tacttcgtag gtagcagaaa
    19021 aagtatccgt tcccgattgc tcgatatgcc atacaatcac atctgtaact gtggagctgg
    19081 tcggtatgtc ggttctaatg gtatctatat tcaagtcctg caattccttt gtcagataac
    19141 cgctgattgc ctgcgtcctt gcttcaatcg cttctttgct gttattccat gtgtaatatg
    19201 acttcgcaaa gttcttcacg aaattttcta tcccgttggt gtcctgcaag cgaagctcaa
    19261 tgatttcttt ttcatgggtg gtgtgctggt cgatagcggt aaaattctta tacaccccga
    19321 agctcacgct tgcgataagc accacccaca aggcaatcac agttttctta tgtgtgccta
    19381 ccttgacagt acgcaccttt ttttcttttg gttctttgat agtttctgtc tgtttcttat
    19441 ttttcttaaa catatcttca agtcctttct attgttttac tcgtcctgca ccgattaagt
    19501 gctgttgcca gtaactactg cttaggtctg caaatcctat cgggtcgcct gcatggtaca
    19561 tctggttatt tcctacataa atgccgacgt gggttacata cgaaccagcg ttataggtag
    19621 aatggaaaaa taccaagtcc ccagcctttg cctgcgagag tgggagatgt tgggtagcat
    19681 catactgtgc ttgtgccgtt ctcggtaagg agataccagc ttttccataa caccattgtg
    19741 tcagtcccga acagtcaaag gaagtgttgg gattactacc accatagacg tacttccagc
    19801 cttgatactt caacgcttca ttcattacct tttgtgccag ctctcccgac acctgcggaa
    19861 cagttaaata ctgattgact aattccacat agaacatatt tccatagcta taccgccagc
    19921 ctccattctt cgcaacagct attggattgg tgtatgttac tttctttcca cccgattttt
    19981 cacgggcaaa gctctctgca aggttgaaag tgtgtttatt tccttttcct gccacatatc
    20041 ccacataacc accgccatag ttataagact gtatcgctac attcaaatcg tccatacctt
    20101 gatttttgca ggacgaaagc agggacgcaa aatatttaca cccctgcttg attgaacttt
    20161 ctgtatctaa tgaattgggc ggtagtccca gactttcaga gctttgcata acgtcctcgg
    20221 ctgtaccgcc actttccact tgtatgattg ccagcaacac attgacatac tcggaaatgc
    20281 cgtattctct ggcgtacttt tccaccatag gctgatgttt caagacttct gcggataagt
    20341 tcatacccgt aatgccagaa gaaaagttgc tgttctcgtc gtcgctgtcc gcactaatca
    20401 acaccccaaa gaaaagcacc agtgaaaaga ggataggaaa cagactgccg ataatagcaa
    20461 tatgtttcag tttcattttt tcttctgtcc tttcttcatt acaaggttac gggttttctc
    20521 tgtggtctgc tggtttttct ggacgctttg cgtctgctga acttttgtcc tgcggtcttt
    20581 ggtgtcattc tggcgtgttt cctgcgatac cactttttct acattctgcc tatgtactgg
    20641 ctgtgtaggc tggctcgttt tcctatcaga agtacccgaa gataagggac gctctttgat
    20701 aaccgttgtc ttgactggct catttgtttt tgaagtcgtc gctgtggcag ggcgtttgac
    20761 ttcctgcgtt ttttcagcac tcggctttgg aatggttgaa gctgtggctg gtcgctcatg
    20821 gggacgggta gctcccgttg tcgctgatcc gtcagccttt cgctgtgcct gccttgcttc
    20881 ttgtgccttt tgaagctcca tacgcttatc ggcgatattc tgcctatgct gttcccgttt
    20941 ttccatacgt cccgactgtc tggactgctg ttcctgcacc atgccacgct tgaagtcgga
    21001 cacgctggac tttgcctttt cctttgcgga gtggacggca taagcggtct gtgtcggcat
    21061 atccttgata ttctctttga cagcacttgc cttatctttc actttatttt ttgtatctaa
    21121 gacagcaccc acctttgaac ctacacgctg tcccatgctg gaagatgtat taccccgact
    21181 atcttttgta gctgtgttcc ttgtcgcttt tctgtttgaa atcgtgctac cagccaccgc
    21241 accagcaaca ccaccagcag taaccgcacc agcaagccgt ctttccatac gtctagccct
    21301 atgtgccaag tatagatatg gtctgcggaa aattcttcgt cccatgcttt gactatcatt
    21361 agcgttcaaa gagaacatac tcattaagtc acccagcttc atgtagatac cagcgaaaca
    21421 cactatctgt aagaacgcca ccatgaaaaa cggatagtcc gtggatatgt tataaaacat
    21481 actggaaatg ctgaacgcca ccgttacaat gagcgttatt cctgctcgtg tcattatggt
    21541 attaaacacc ctcacgattg cctgctttgc catgctttca tagctcggta tcatggaaag
    21601 taaaaagctg ataggcagaa acatagcaaa gataataaaa agtatctggc tgaataacat
    21661 catgcccgta agcaagaata caaatatcgt tatccctaag ttgaagaaca gtaggaagaa
    21721 caccatacct aaacggttca cgacctgcgg tatcgtcaga ttgttgttgt cgttgtcctc
    21781 gatttctgtt ttcacgactt cctctctggt ctttccgtcc tcgtcctctg ggcttgccga
    21841 taccagagct tctacacggt ctgccccgat ttcttctgcg ttgctgttcc cgaattgtaa
    21901 gagtaaccac ggctgttcca cttgaataga aaataggctg tcccttatca agtccacgct
    21961 gtccttgccc ttgctgtcag agtttgggag catgattttt gttcccaagt ccaacgaagc
    22021 ggtactaata tcacttgaaa attcatttat ctttttgatg tagtcgggag cgtaggcaat
    22081 aaacgaagcg gacagcacga acaccaccac aaagttaata acggcatgaa gtgccttgct
    22141 ggtttcccgt tttatcagtc ccgtataggc aacataaagt cccaccacta agataatgag
    22201 aagcaggaaa ccaacataga agcccgtaga agaaaaaccg ttctgtgtta cgcctgcaat
    22261 ggtctgtatg ctttttccga tactgtctgc catatcgtta atgaagtcca gtttataggc
    22321 ttcctgcacc acatagcccg tggcattgct taaataaagg cttatcgtcc agacaaagtt
    22381 ggtaatgcag taaagcccat actgcaccga ttttccgata ccgtccagcc agttccacgg
    22441 aagccacgac cagctattat ccacataaaa atcaagctgg taattcgaca gcggatattt
    22501 tgagtaaaga ttttctgcat ttatggtatc gtccacaagc cccgtcgcat gagccaccgt
    22561 ccccagaagt gaaagcagga taagggagag tgccaccacg aacagaacca ttttgagaaa
    22621 gtggaaaatc ttcttttttg tgaacgcacc ttttatcctt tctttcatca ctccacctca
    22681 tttctctgta cgggcggtct ggtatcaaag gcgtgtagca gttcttcaaa gacggggtgt
    22741 atctgcacca caccgacacg tccgtataag tcctgcaata agcattgtcc gttctctaaa
    22801 tcacgcaagc gtttctggtt gttctcgtcg tccttgtcga taccaaaaaa ctctaaggtc
    22861 tgttttatct cgttaatgtc agtagaacga aaggcaaact tcaagccgat attgtttttc
    22921 agactttcct ttgaaacatc ataggcagac tgggtaacaa aataaacgcc tgcctgcata
    22981 gctcgtccag cacgaaccag cttgttagag agcgtttctc cttgtgccac atttaagaac
    23041 gcccacgctt cgtcaaggtc tacaatctta aaaatacttc tgtccgaatg gataaaatca
    23101 agggcaaagg tactaatcac aatcagcata gaaaccgata ataattcaat ggtcgtgtat
    23161 tcctcaaaag tggtatcttt atctggcagt acaaggtcag ctacttgaat gatgttgagc
    23221 tggttatcca gactgatagc attttctacc gtcccgtctg aaaacagcag atgtgcaaag
    23281 tcgtagtccg tgaagctgtc gatatggtct gcgatatttc ttgatatggg cgtatcttca
    23341 cggcgtagct cgtctatcac atggagcaag ccccgactgt tgctctgggt aacggagcgt
    23401 accgctttac gaagtacggg gaatttttcg ccgtccctag aggaaatacc cgtaaggaat
    23461 gttaagatgt cgattgccag actttcagcg tcttttacat tcttcataat cacgaatggg
    23521 tcaagaagtc ctgcatttcc tttatcgctg gttaagttta cgatattgat ttcatgtgcg
    23581 atttctggaa gtgtttcctt ccagttgcca cgctctgatt ttgggtctaa gataaccgcc
    23641 tgtcccccaa agagaaccga ataatacacc agaagattgt tacagaacga ctttccaccg
    23701 ccaagcgaac cgacaaaagc agacgcaagg gcgttggtaa ctgtaccttt tataccttga
    23761 ctggcaaggg acggttgcag gtacacattt cttcccgtat caacggaata gcccatatag
    23821 atacccgtag tttctcctaa ctgctgtgtc gcaccaaagc caagcccagc taaaaaatct
    23881 gattttacat actgcacata atcattgata tatcgcttgc tggcaggtaa aaattcagaa
    23941 tgaagcccca gcatatcccc agcaggacgc accagcttta cattgaggtc gtcgtaaaag
    24001 tccttgactt catcacaacg gcgttttaat tcgtccaaat catcagccga tacacgcact
    24061 acataagaga gcttatacat actttctttt gtctggtcta agtccgtttc cagctcatcc
    24121 acgctgtcta atgcgtccac cacatttgaa ctggtttcac ttcctgcttg ataggcgtga
    24181 ttatccaaat ctttcaattc ctttttctta tttcttactg tggtaagagc ctttcgattc
    24241 tccacgattt ctacattcat agaagtatca acggggaatg tgaattgctg ttgttggaaa
    24301 tagaagattt cacttgacgg aaaatcaagc tccccgacaa tcgcattgac ggtaaagtag
    24361 gacacatagc tttccttgtc ctcatgttcc aatcgtaaat accgctggct ttcctcaatc
    24421 acacaccttg tcggacggat aaggtcgtag tattttatca gcgtttcttt gttcaatttc
    24481 ttctttggta actgatactc ataatcttca taggcgatac cgttcctgcc gtaaagatgt
    24541 tccatgagat acccaaaatc attgatttcc aagcgacgca ccttgaagcg acgggagatt
    24601 ttattttcca gtaacttttc cattttcatg taacggttga tttcatcatt cggcatggaa
    24661 acaaaatcat tcatcagcgt gtggttcact tcatggagaa attccgtgaa cgtcagccac
    24721 gccgattttt ttatgttctt cagattgagc tgttcttccg taaccatgag cttaaagcca
    24781 agaaaaaagc ggtagtccac ttgattgtca ccaatcatag atactaacgc ttcggtctgt
    24841 tcgtctattt tctggcaggc aacttccttt aattttccag ttaccagctt ctttgactgc
    24901 tcctgcatac tatggattga gctttccgta gcaatctgca acgcatgaat tttaccctca
    24961 cgggactgtg cgataagctg tcggaaactg tcatgcacga taaatttctg ctctgcggat
    25021 agaaaagaat agttgtacgg tatcagctca tagtaagcga acacctcatt gtccttgttc
    25081 cagacaaggt tattgtcaat atattttatc gggaacatag ttcacactcc ttactgccgt
    25141 gattgtttca ttcaaggtct gcttatgcag ttttacggct tttcccgcat aagtgatttt
    25201 aggtcgcagg gcataggtta tctgtgattt caaaaagctg tacggcttct ttccgtcaaa
    25261 ggttttctgc gacataaacc aagtgagagc aacgggaata ccgaagtatt tgagaaatgc
    25321 tccctcaatc atggaaagtg ggggaatatc cccaaacaga atgatgataa attccgtaat
    25381 cacaaaccat gtaatctgtg taaaggtaac gggaaaaggc aggttaaagt cattgattgc
    25441 atacaagact ttttccacgt tccagatacc cgtataagat ttaatctttt tcaagttctt
    25501 tcagctcctt tcgtaagttc ttcaaagaga aaaggacagc cattttcaga ctgtccctta
    25561 aaagagaaat acgctgtcag tagcaaccat gccgttgctg atcttccagc gttaatatgg
    25621 tatctcgcat atccctcggc ttgtttcaat aaagcaaccg cccatatcca aatctcgccc
    25681 gtaggcttca taatcaatgt agttctgtaa gctgggtgga atgtccccaa gtgcctgcaa
    25741 ttcgtctatg tagtaatagg caacatctgt catggtttca cagtcgggat aataataaat
    25801 atcgtccttg tgttccacga cttcttccag cgtcccgtag tgactgataa attcgtccag
    25861 acactctacg atatagtcgg gaagttcctc tatcatttca tacatctcat tgagttcttc
    25921 aatggaaaca tactcgccaa tcgcaatggg gaagttgtcg gtatcatgga tagcgtattc
    25981 ctcatactgt tcattcaagc cgattttttc ttttacatct tcctcgtcaa tggggaacgt
    26041 gaaccaagca ccgactaaat aaccctcatt gtatttaccg agattagcaa tgtaaaccgc
    26101 catatcatct atcataagca ccacctcatt tcctactctg gtagttcaaa gataccgtgt
    26161 tccgttacta agaatttccc gtgttcctgc aagtgagaag cgtaggcttc aaagtcaaag
    26221 tagtattctt gacagtcctc ggataaatgt ttgaacgtcg agtcattcat cagcttttgc
    26281 cttgcaacat ctatcatgct tttgcagtcg ggataaaccg taatcacgtt cctgcaaata
    26341 taaagtgctt ctaaattctc aaacaccgta agtagtgata catattcttc ctgcatatcg
    26401 cttgaaagct ggcggtacat aaactctaat tcgttaagct ggtacacgct tgtatgttcg
    26461 tggacttcat cagcataagg caacaccttt tcgataatgc ggtacgccgt gctttctgca
    26521 ccgacaccta acttttcttc aaactcggca acatctatcg gcaggtcgaa ccagtatgat
    26581 gttgtttctt cgccagttgt ttctctcgtt tccaccagca cccttgtttc ctgcattgtt
    26641 cttcacgtcc tttctgttgt ttcatcacat cttttaaggt ctggtacggc ataccaaaca
    26701 caaactttga aaaatcttct aattgtcttt cttcataatc ggcagggctc agccgtttca
    26761 tttcacgcca caccttacgc ttgtaagagg gcaggtcaga aatgtattca cagccgactt
    26821 ttgcctgcat atccttaaaa atatcgttca tgtaatcact ccaatctgaa attttggtat
    26881 gaaaaaagca gtcaatcata agactaactg ctttgtgtga atattaaatt atagcttatc
    26941 ctaatctctt tttaatcttt ctttttagtt tgaaaaagaa actcgttgaa tattgctcgt
    27001 ttttcactaa tagttcatag gtttccataa cattgattgt atatacaaaa cccaacattc
    27061 caacagcaag accgaaaaat gcaaaggaat gatattctgt ccatgaaata tagcctttca
    27121 cagtaaatag aaagaaaatt agtcctgcta taaagagaac agtacaagga acagcaacta
    27181 ttacatattt tttctttttt gaagtgtatt cgcttttcaa ttcttttaga tattcataat
    27241 caagtagtaa aggttcttgc cttaaaatgt tgtattcttg tttttcataa aaaattgcta
    27301 cgacaaaaaa tataattcct gccacaaagc aaaatgccat acctaaataa cgccaagtca
    27361 tattatctaa aaacaaggta tatggtattc cagcaaaaat gcaaagcata acccctaaag
    27421 caagataaag tcctaatttt ttctgattag cgataaaccc taccgacatt tccttgctta
    27481 cataatatcc tcgttcttca ctattcttta cattcttttc gtctttcaaa agataatccg
    27541 tagatacttc aaaaatgttt gataattgta aaagcttttc tgtttctgga aacccttgat
    27601 tgttttccca tttactaatt gcctgcctag tagtgcctat ttgttcagct agtgcttctt
    27661 gcgatattcc tttttctttt cttaacttga agattttttc tcctaaactc attgtgtgat
    27721 acctcctatg atttgatgaa taaattttat caagccataa tgttatattc tatatttttt
    27781 gaaatgcaca ttagcaactt caagttgcaa ccttgtataa tatacaaaat tcatagattt
    27841 ttctggtaag tacagcgttt tatgtaatac aacattcaaa ttataacatt cgcatatcag
    27901 taccacaata aaattttatg caccgataat cttattgaac agctctaaca atacatcttt
    27961 cacaccgcca gcattgaata ccaaaccgac agcaatcaag gcaactacca agaagccgat
    28021 aagtttggaa aactcacgct tgaaccctaa gtagataccg ataaccacaa tcgccataag
    28081 cactaagctc tgtgcgttac ttaaaaacca ttgatacaaa ttttgtccga aattcattta
    28141 attgtcctcg ctttcttcta attgtttcat tttatagtca gcttctttgc cgacattcaa
    28201 aatacacatc aagactatgc cgatacccat tcccatagat accagcagga agtcttttaa
    28261 taatacccac atttttcatc actcctaaat ttctactaaa tcttttgcag gggtcgtctg
    28321 ctgtttgatt atcatttcat gcttttctgt gagctttgcc tgctgttcga ttgtctccat
    28381 gtaatctgta ccgtttccct tatcaatctt tttcagcatt ttcaaggtcg gtgctacctg
    28441 cctttgtacc caacgcaatg tacgatctaa ggtataaggt tctggctttg tcgtcagttt
    28501 cagactttgc cgattatcgc cgataaacca agcccagcgg tcattgagtt tccagtcatt
    28561 tttccgcttg tctggttctt catcaacaaa ccgtacatac tgattgatga tagaaaaggc
    28621 ggtctgctct gcgtcgtaat acgtcagcaa atcccgtact gcataatagg cacgttcatt
    28681 tctaagccgt atctcaaaac ggttgataat atcggcttct tccagaggtg tccctaactt
    28741 gacatactgc tcatagtcct tttcatagat acagaaatat acatcagatt tcagcgaacc
    28801 aagataaagg gtacgtccca tatattctct gtcgtcctcg ctgtgcttga taagctcgcc
    28861 cgactggtaa aacttataac ttctggactt tccgatatat tcccgttttc tgcatttttc
    28921 cgcaagctct ggaatatcca aaatgcccgt atggtcgttg atagcaaggt cgatacgctt
    28981 catcacgcca ccgtctatga gtgcgtccat gagaaagtca taccagcttc tttgctgtgc
    29041 cagcaggtaa ctctcaaact gtcggcaacc acgccccttt aactctaaaa ggacaccttt
    29101 ttcttcgtca gccgacgtat agataaagat gtcccctaaa gaataatgct ccgtataact
    29161 gtaatgtccg taatcttcat ggagcatata gttgatattc agttttaata tatctttgat
    29221 gatgtgctgt atatccagcg tgggaaaacg aattttcaca taatcaaaca gcatggtaag
    29281 cggtgcttct ggattgaacc ttgccagttc tttatgcaga gtttccagaa gttcctttga
    29341 cggcttcatt tttccgcttt ctatcttgtt gagatattcc cttgtgatac cagaagccac
    29401 cgccagcctg ccttgtgaga taccgtaagc aatccgtttc tcccgtaatt cttttatcca
    29461 ttgttcttca ttcagaacca tacccccaat ctgtcaagtg tgaagttttt taaggcgact
    29521 tcacagccga tttttactgg gaaatcatgc cagaagcctt atgtttccta tgtttttcgt
    29581 ctattgtcta tttgcaaacg tacccctctg ttagataccg aggggtttta cctgctggcg
    29641 tggcttacgc cacaccagca acggctagtc cgtgccgtcg cctttcgctt cgcacgtcgc
    29701 cgtcccgtcc tgccttgctt gtgcaagaga gccgatagtc tgcaaaaagt catgtccttt
    29761 cgggactaaa ggcgtgtaaa attcgctgat aacgcttgtt cccacatcac aatagccacg
    29821 ccccttgata cgcttctgga aaaactgctt tttcacatct gaaccaaaca gcataccgta
    29881 acctaattcg ctgatacgcc caagtcccac gcggaaattg aagttatctc tgataccgtc
    29941 cgagaaatac tttgcgtctg gacgctggca ggcaacgata aggaaatatc ctgcttgccg
    30001 tcctaacata acgattttct ttaactggct aagtaagccc acgctttctt ttgtccccag
    30061 catttcaaaa aacgccacat attcatcaaa gataagaaag cagggtggca gtcccagata
    30121 agcgtagttt tcgcccgtct tatagttcgg gtgtcgcttc atttcctcac ttcgctgtac
    30181 cataccctca taaaaggcat tgacgcaatc aatcattttt tctttggtgt gatacacatt
    30241 ttccataact gtccctaagt ctgctaagtc cgcatttttc gggtctaaga tgtaaaggac
    30301 agcgttggta tgcagtaagg cttcaatgag cgtcagcaga aaataggttt taccgccacc
    30361 agtcccacca gcaatcagag cgtgagggag tgcgtcatat tcccagacaa gatttttcat
    30421 caatttaaga cagccatttt ctgcccgtac ttcatcaata gtaatgcggt tcgctatcat
    30481 atcataaagc agggtatatt cgatataacc gtcatgtaag gtcttgtcgg tcagctcaca
    30541 atataatcca ctttccaatt tatcctctaa ccgtaaaagc tggtcttgat attttcccag
    30601 cgtaatttca cagcggatat gaagcagtcc cttttccatt tgataataaa tctttggaaa
    30661 ccagacgatt ttttcccttg atctgctttg caggtcagta aaaaaaccgc tgtcttgtac
    30721 ggtatcggct tcataccact tattttccag tatcattctc gccagctttt gacggtgcag
    30781 gagcttcttg aaactgtcgt aacagaagcg gtaatacaga aaagcgacca atgcacaaac
    30841 accagtcgct atcagtatgg ttatgaagtt gtaaggggaa agcgtcaagc cgttctctaa
    30901 caagctgaaa tgctcccagt cggtacgcat gagctgtttg atattcagta gcaggagaac
    30961 cgccacgaat aaaaacagaa gcgtccctat ggaaaaatgg tagacaaggt gcttgtcgct
    31021 ggctctgata cggtgtcctc tattccaaat cttacgcata aatcattcac tcctttctgg
    31081 gtacgaaaaa agcagtcaat catatagact aactgctttg taaatgttgc aagatataat
    31141 cataaatctg catttttagt tatattaagg tcgcacatat aatgccaatt acaccaatag
    31201 ccaaaaataa cagtccaata cctgctgatt ttttacgttt ttctaattct tttgagttgc
    31261 tttcactcat atttacaata gggtattttc ttcttccgat tagatacata agcaaaccac
    31321 ttgaattatt cccactcatt gtaaatactc cccaaaattt agggtgcttt aatcctctgg
    31381 ctgttgcgtc tataacagtc atatgataaa tctgaaacac aacacaaaac gctcctacaa
    31441 gtgcaactgc tccacaaatt accgctaaaa ctatgttcaa attcataaca aaatatcctc
    31501 cttaaaattt tttgattatc tggtaaagta atgctgatga agtgataacc ataagacaaa
    31561 atattccaac taagaaataa cggttatctg gtataaacat atttaatgct atcagcaatg
    31621 ctacgacaat gatatttaca ataattgccc atatcatttt ttggttgctt tttacaacat
    31681 ttgtactttc ttctaaatgt tcaatcattt tttcatcacc ctttagcaat tcgtcaagac
    31741 ttatagaata taggttgctc aactctatta cattgataat gtctgggtaa gatttttcat
    31801 tttcccagtt tgaaattgtc tgccttgata cattgatttt ttctgcaaca ttttcttgtg
    31861 tcattccaga ctgtactcgt gcatttttaa gtttcttacc tatttccata ttccgattgc
    31921 tcctttcggt gtcattgtca tatattaacc ctcaaatgtc tatccaatat cttcgacaaa
    31981 tccttttttt ccttgtcaaa attattttac atagcttaaa tacggctctt ttggctcatt
    32041 atactgtaat ttcagagtat aatctatgac ctagatttat tttttcgtct gtccctgcgg
    32101 tacatggttc tgtggattgc ctgcgttctg attgccttta ttcttcaaca caatatcctc
    32161 tgcctttaca taccagtcca catctgcacc accaaacgtc tttcttgata ccgtatcggc
    32221 tacgggattg acaagctcta caactgcatt gtacggaaat tcccttaacg gcacttctgg
    32281 cggtacggac acggggataa ttccaccatg cagactacat ttcaagtcat agatacgctt
    32341 tttaagctgg gtactcggtg tcccgtcctc attctgcata aacacatcac gcaccgctgt
    32401 aaattttaat tcgccaaatg ttttctcttt gtcaataaca aacccgtttg ataatctcat
    32461 aaattcttca ctcctttact tttcttcaac tgctacaata tcatcagcaa ctaatacata
    32521 atcggtatgt cccatatccc cgattgcgta acctctgccg tacaacttcg gattgacaag
    32581 ttttactttc tgttcatact tgaacttctt ttcgccagcc tgcacgggaa tttccactac
    32641 aacattttcg cctttctgta cgtcagaata aaggttatag cttcgtctgg ctaagaccct
    32701 gcggttattt ttatctcttt caaagatagg ctcgctttct cctgcaaatt caagagttcc
    32761 aaaagactgt gccatatctg gcacgacata tttcatttcc ataatgtttt acctcgtttc
    32821 tttctatttt gataaattga ttgtaatttc ttattctggc ggtttgggaa gtaccagcaa
    32881 tcccccagat agtaaagggt aaaatcaatg cttcgcaccc ttgactatcc gtgttcttgc
    32941 aggttggtgg cagacaagcc agaataagca aaagtctgcc gtttgacagc ttcggcggag
    33001 agcgtgattt ttctttcatc ataccgttac cgccttatga tttcttcatt ttacggcgtt
    33061 tgtaaagata tgttccagcc agtccaccag cagacgcaag caacatcaca agaaatgcca
    33121 ttacatttgt actgtcgccc gttttgggac tgtcgctcgg tctgctcggc ttttctggta
    33181 tctgtggtgt ttcgggtttc tctggttctt ctggcttttc cttaaaggta atcgtctgtc
    33241 ccttatcctc aatatcctta tgctcggtaa ctttcttcgg ctcgtctgga ttgcttaaat
    33301 cgtacaattc ttcaaaagtt acaagctgtt taccgtcaag agaagtagcg tcaaacgtaa
    33361 aagcaacttc cactttcata gtttcgctgt cagcagtaaa cgtataatca ttttccacac
    33421 attttccatt gataagaagt tctgcatttt cttctttcaa catctgccag cccacaagtt
    33481 tgtactgtgt accaacttct aagccctcta aggttacggt atcaatgatt gtaacgtttt
    33541 ttccagcttc aagctctttg ttgccgtcct tattcgtagc agtcgtatga attttgatga
    33601 tacgctctgt gattaaaacg gtctgcccgt cgtcctcaat gtctttgtgt tccgcaactt
    33661 tcacgggttc gtctggattg ctcaaatcat acaattcttc aaaggtaaca aggttcttgc
    33721 cacctaaagc agacgcattg aatgtatagg caatttccac tttcatttct tcctcatcag
    33781 cgacaaaggt ataatcattt tctgcacgtt tcccgtcaat gataagctcg gcgttttcct
    33841 cttttaacat ctgccagcct ttgagctgat attttgtgcc ttttgtaagt ccgtctaatt
    33901 tgacagtatc aacaatcgta atctcttttc ctgcaaggat agtcttttcg ccgtccttgc
    33961 tggtcgctgt ggtatggata gagatttctt tttcgtattc atcagtcagc gttcctaaat
    34021 caatcaaaag attatttctt gataccacga tttcaaaagg tgggataagt tcaaagcctt
    34081 tgttgctttc agagcgtaat tcctcaatga cgtaagtatc gtaaggtaac gcacctttgc
    34141 tgtcgtctgg ttcagaagtt ccaaaccaca caccgtcctc gctggtcttg cctgcgttgg
    34201 tattgtgctt atgagaagcc cagtcagcag aagtggagaa ctgcccgtta ttgtcagtta
    34261 ccacgatatg actttctcct gtcgtcttgc ttgtgatcct aaagggaaca tcagcaagac
    34321 gcttgtgtgt gcctgcaccg atttttacac cctcaatatc tccacgctta atctgattat
    34381 agatagaatg ggctttgtcg gttaagtcca cgatttttcc attttctgtg attgtaaaat
    34441 caatcggctt tgcaccgtca gttaagtaac cgtcgggagc ttcactctca acaatacgga
    34501 attttccata aggtaagagg tcagcagaag tagaagcgac accctcaata tcggtacgaa
    34561 ttgtctttac cacttcattt ttcttgtata gcttgccctc aaccaaaaca gaattatcat
    34621 ttaaggaaat gatgtcaaaa gcagtatctt tcaaggttgc acttccttgt ggcttcatat
    34681 cgcccgtttc taaatctcgc ttctgaattt tcacaccgcc acggataacc ttgtctgata
    34741 cggaaaactg gttacttcca gataatacgg caaggtcgtc gtcctcggta atctgtgtta
    34801 catataagcc ctttatctgt tcggacttat cgccagcctg catatatgca ccctctaaca
    34861 agtagccgtt tggagctttt gtttcttcta tggttagtgt cccaagtgga agaacggctt
    34921 ttccgtcttg catatagaag ctgtcgccag acaccttgta tgcgtccgct aattttgtga
    34981 tgtaatgagt tgtcccgtag ctgtctgttt cagcgattgt ctttgtaacc catgttcgag
    35041 tagcttcggc agggagattg tctttgttat agaagccagc ataatacttc catgtaaatt
    35101 ccgcacctgc taaagaagcg ttcccttgcg gattgtcttt ctttgtttcc atatctatct
    35161 tgaaaagctc aatcaaagtg tccgttactt ttggcgtatc agatacgttc aaagtcgccg
    35221 tctttccagc ttcaatcttt aaggaatata cagttttatc tactttatat cctgctggtg
    35281 cggataattc cttgatatag actgtgcctg cttttacctc tgcaacatct gtatttccgt
    35341 tttcatcagt cgtaagggtg gcaagctgtt tcgtgcagtc cttatcagta aagacaccat
    35401 aggtcgcacc agcgattgag taattcccgt taccgtctgt aatgctggca ttactggaag
    35461 tctttttaag tttcgcattt ccaacattca gcttcgccca gaattgcccc aattcctgcc
    35521 cctcgccaga gtagatataa ccgccacatt catagcgtcc tttattttct ttgacaaagg
    35581 ttcttgcacc agagaaaact tcgtcctgcg ttgcctttgg aatttcatca taagaagctc
    35641 gcacgttatc gcattgccag ccaagatgta cgctcaatct ctgccagact acacattgtt
    35701 ccaacagata gacttgcttg tagttcagtt ccttgtgagc ttcgccatac tgtttgacat
    35761 actctaagga taacgccaca tctgaaatct ggttggcatt catgcgtgag cttgcgtcag
    35821 ctctggtctt gtagccattc ctaaaatctg tgttaatgtc gatacaatag gctgttttgc
    35881 cctcaacttt catcatgccc tcattgaatg tcgagccgat agaaccgtca ttcattactt
    35941 tttcaatgat accgacacgc tctgcacttt ctgtccagta ttgcttactt tctgcatgaa
    36001 cgggtgtagt cggtaaagca gtaacgatag ttgcaagagc taagaagccc gtacacaatc
    36061 gtttcaatgt ctttttcata aattttaata ctcctttcat ttttggtata aaaatagacg
    36121 ctcatttctg aacgtctata acatacgagt atcttgattt ctagtgataa atgatttata
    36181 gtttgtttgt aatgttttct gataaattaa agctttaaat tagcctttgt tgctaacaac
    36241 ctgctaacac tctacggtgt atgagggtac accagagagt atcaacaatt aaacataaca
    36301 agcacttatg ctttaattta cggcattaaa cgctcccaag aggataatcg aacttctagc
    36361 taccacggtt cgcagagaac tcctaattct gaagcataag atacagttgg atatattgta
    36421 gacaccacca tacttaaaat cataattaaa ctaattaata atgccttttt atttcctttc
    36481 ttcatataaa ttttccccct ctaggttatc tatagtttat aatcttccat taatatcgta
    36541 tataaattaa tttttactaa aaaattatat aaaaaaagct atgaaaataa ataaatttat
    36601 attcatagcc ggttgcacca ctgactcagt atatccataa ggtgcccaat aatagaatat
    36661 acttcaacat ctctataata tttcataaaa acaattaaaa ttgctttatt tatattaaaa
    36721 atacaattat atacttctat tatattatgc ctctatacta ttgtctatct tttttacatt
    36781 ttttaccttt tatgagatta attaataatt aaataaaatc cttatctaca acaattacac
    36841 aattatattc aggatttatc tccttaacta attttgttat atcttctttt aagtcatcct
    36901 ctgacatgtc tttggaaaat tcggaaggat taactactat atcaaatatt aaattcttct
    36961 tgcttccttt tccaactatt ctaaaatcat gcatagattt tacaagacta tttttcttaa
    37021 gtatttcttc aagttctttt ttggctgaca taacttcttt atcttcaaga catattgggt
    37081 ccatgtgaat tactagatat atatctaatt tttcagatat ttctctctct gcagtatcta
    37141 taatttcatg tatttccatt atatctatgt tggatggaat ttctgcatgt atagatgcaa
    37201 tacatctacc aactccatag ttatgaataa ttaaatcatg tgtacctatt atatatttat
    37261 aagataacaa tagctctata atagaattta ccatttcttc atccggtgct tctcctaaaa
    37321 gaggatttat agtatctttt acaagagaga aaccagcata aagtatcact aatgatacta
    37381 cgataccaac atatccatct ataggaaaat tagtaaatct agctacaata aatgatataa
    37441 ctacacaact tgaagtaaat acatctccta aagcatcaag tgaaactgct tttaaggctg
    37501 aagaatcaat tttatttccc acaaatttat taaaacgact caaccagatt ttaatcatta
    37561 tagatacaat taaaagtata agtggtatta cttcaaattt tacaggaatt ggatttacta
    37621 tcctttctat agatgacttt acaaattgaa gtccaaccaa catcaccata aatgctacta
    37681 ttaatgctga taaatactct attcttccat gaccaaaagg atgttctttg tcagctggct
    37741 tacttgctaa tttaaaacct atcatagtta ttgctgaaga tgccatatca gaaagattat
    37801 taaatgcatc agccattata gctatactag atgtgaacat tcctatgaat acctttatca
    37861 caaataaaag taaattggac aatataccta ctacaccagc tacataacca tacttatttc
    37921 taacatctgt attttgaacg ttttcgctat ctctgataaa tgttttgact aggattttgg
    37981 aaaacatgaa acttacctcc ctgcccttta ttttattaac aaattttata atctatatct
    38041 ttatttatga taacataatt tatatataaa attcttaaaa atcactctac ttcagtaaaa
    38101 tattattcta tatatctatt tagatacaaa ttttatattt cgtaaagttt ttatggctta
    38161 gtgtctgttt aatagattct atataagttt ttacaaagat tatttattat gataatatca
    38221 tttttcccaa gtattactta gaatatgatt tttttgtttt gtctttacat attttaaatt
    38281 tatactatat ttatgttttt ataaccttat gatacactat aactataaga ttttaaacgg
    38341 aaaggaatga aataaatcat gttttttatc agtgatattt acacaaaatc acctattaaa
    38401 tttgatactc cattgcaaaa agaggtatac aaaatattac aaaagttaga tattgacttt
    38461 gaacgtgttg acacagatga agcaattaca atggaagatt gtgttcagat taataaaaaa
    38521 ttaaatatga aaatggtaat ataaaacaac attaaacaaa caaaaaccgc aaagttacaa
    38581 tatatagtac tttgcggttt cataagaatt aatttttact attagagttc atacttctac
    38641 tatttttcat tgttctaagt attagcttta caaattcatt tgacaataat atagtggatg
    38701 caaatagtat tatttttatc cacatatcaa agtccaaaga aacagaatta aagaaactct
    38761 ggaacacttg tgtaaatatt atttgaatta tccctgtaac tactattatt tgcaatgcca
    38821 gtttattttt aaagaaattt ggtattgtac tatttaaacc aaattctcta caatttaatg
    38881 cattaaaaag ggcactgaac gcaaataaag aaaatataac agtaccttgt tcttctgatg
    38941 tagctcccaa tatattaaaa gctgattgag taaatacaat tgtaattata agaatagcat
    39001 ttataataat atttacaaac atagacctag ctattattcc agaatgtcta tttattggtt
    39061 ttcttttcaa tacataatct ctaacaggtt ctaatcctaa tgctagggct ggaggaccat
    39121 ccattattat atttacccat aataattgta ttgtagtaaa tggcatatcc ttaccagtaa
    39181 gttgagatat tatagcaatt ataaatgcta ctatatttac agtaagctga aactgtataa
    39241 atctttgaaa gttctcatat atacctcttc cccattttat tccctctact atagtactaa
    39301 agctatcatc agtcaatatt atatctgctg catttttaga tacttctgtt cctgaaatac
    39361 ccattgcaat tccaacatct gcttgactaa gagctggggc atcatttata ccatcacctg
    39421 ttacagctac aacttcgcca cttttttgaa gcgctgaaac tattctcatt tttgtatctg
    39481 gtttacttct tgcaacaata gatattcctt taatttcttc tctcagttct ttatctgtca
    39541 atacatctat atatgttgct tcaactgcct ttttcccatc atttaataat cctaattcat
    39601 ttccaatagc cgttgctgta tttatattat ctcctgtaag catttttaca tctactcctg
    39661 cattaaatgc tttatcaatg gaatctttga ccccttctct caatgggtcc actattccta
    39721 caaatccact aaaaactaaa ttatcttcct tcatataact tcttgaattt cctattaaat
    39781 tcaattcacc ttctgaagtc attgcaactt ctgttttaga attactcatt tttttataag
    39841 caaatcctaa tgttctcata gatttaattt gtaacttctt aatctcatct aaaatacttt
    39901 tttctacttt tggagttatt ggaacaatat tttttccttg ttgtacataa gaacattttt
    39961 ttaataatac ttctggagca ccttttgaaa gtaatatatc tgaatcctct tgacgaatta
    40021 atgttgacat cttcttttct tctgaactaa atggtatttg tgatatcaaa tacgtttgtt
    40081 ttctcatttc attatagttc ttatcattat gatataaaag taatgcacac tctgttgcac
    40141 ttcctatata tttaaatgaa ttatcttctt tttctatatc tgcagttgaa tttactatac
    40201 agttttgttc aaaatagctg ttactttgat actctcctcc acttatatat ttaccatcaa
    40261 catatgctac ctctaccatc attttgtttt gtgttaaagt ccctgttttg tcggaacata
    40321 ttacagaaac agaacctata gtttcacagg cttctttctt tgttactaat gcatttatct
    40381 ttgccatctt ttgcattgtt atagctaagg taatatttat catagttggc aaaccttctg
    40441 gaacagttgc aactattaat gcaatacaaa ccatatatgc atttttagca ggttctaatg
    40501 attgtaagaa agataatata ctgcttgtat ctaaaattaa catacctttt ctctgcatct
    40561 gtataatcat aaacatacac aataaagtag ctattacgcc tgatattcct gctattttag
    40621 cacccaactt ccctaattta atttgaagtg gagtttgctg gtcttcattt gataaattct
    40681 gtgctatctt acccatctct gtcttatcac cagtatgagt gacaaccatt atgcccctac
    40741 catatgctac aagagtccct ccaaatacca tattgacttg ctttgcaggt attgcatcct
    40801 gttcgattat ttctgtttta gaataaacta cttccatatc taatacagca tcagcattct
    40861 tagctacatc atcagattct cctgttagca tatcttctct aagtttcaaa tttatagact
    40921 gtataagtct tccatctgct ggtatcatat ctccagtttc tatatataca atatctcctg
    40981 gtactaaatc attttttgat attttaatta tctttccatt tctaagtact tttacttcta
    41041 tattttctgt taattttgac agggcctgag ctgctttttt agattttcct tcagttatta
    41101 tgcctataga aattccaata aaaatagccc ctaaaatacc taatgcatca tgtaattctc
    41161 ccacaaaaga acttattact gctgctccta tgagaattaa tatcataggt tctgtcaaac
    41221 tttctcccaa ctcgtcccaa aatgttctac cttcttttat agtaaattca tttaatccat
    41281 atctaagctt cctttcttct acttcattat catcaagtcc aatttctgga tttgttttta
    41341 agtattttaa tacttctttg gtaggtttat tataatacct cataacttta tcccccttgt
    41401 taattatcta taaggaatta tatgcttatg atagcttgta tattactttt caaaaaaaat
    41461 aaggtgttga ttaatcaaca ccttatagtg gttatatatt attaaatttt atgttacagg
    41521 ctttataaat tgtgagctat acaaatcaaa atatttaatc ttcaaaatac ccttctggat
    41581 ataatgggaa cttagatata agttcttgtg cctctgcttt acattcatct aatacttctt
    41641 tattatcaat attttctgct accttattca ttattcctgc tatctgtata acttcttttt
    41701 ctttcagacc tctttgagat attgatgtaa gacctatacg aacaccacta gttacaaatg
    41761 gtgattgtgg gtcgtcaggt ataacatttt tattaactgt tattcctaca tattcaagag
    41821 ctttttcaaa ttgttttcct gtaagattct tacttcttaa gtcaaccatt actatatgat
    41881 tatcagtacc tccacttaca atactaaaac catgtgaaat cagttcagaa gctagagtct
    41941 gagcatttac tacaacttgc tccattatct ttttaaattt atctgtactt aaatataata
    42001 gtgaaaaggc ttttgctgca actgtattaa gatgtataga ccctaatgtt cctggaaaaa
    42061 cacctttatc gagtttcttt gcatgttctg ctttacaaag tacaatacca cttcttggtc
    42121 cacagaaagt ctttgtcgta gatgaagaaa caaaatctgc ataaggtaca ggactaggta
    42181 ttactttcgc tgcgactaat cctgcaacat gtgccatatc taccataaag taagctccta
    42241 cttcttttgc aacctttgat attctttcat aatctattaa tcttggatat gaacttgccc
    42301 ctgcaattat aagttttggt ctacattctt ttgcctttgc ttcaagagca tcataatcta
    42361 tacgtccagt atttgggtct accccataaa attcatattt ataaatgtca tgtaaaaaat
    42421 taactgcgct accatgtgtc aagtgtccac cttggtcaag acgcatactt aaaacagtgt
    42481 catttggttt taatattgat gaatatacac aatagttagc tgtagaacct gaatacggtt
    42541 gtacgtttac atgttctgct ccaaatactt cttttgcacg ttttattgct agggtttcaa
    42601 gtttatctgc ttcttcagaa cctgcttgaa aacgagcccc tggatagcct tcttctgttt
    42661 tatttgtaaa tacacatcca cttaattcaa gtacttctgt tggtgctgta ctttcagaag
    42721 ctatcatctc aatattgtgt tcttgtctaa ccaactcatc agctacaatc ttgtataact
    42781 ctgggtctga aatttttgtt gtttttaaca taataatcct cctcttaatt aaagaatata
    42841 ttttattagt ttaatcttca ttgataaatt tattataatg gtctataaaa cgtaagatat
    42901 tcctccacca ggtcctaatg gtatgccaat acccatgaat agtaacaata ttattatttc
    42961 tattaatgta aataccactg agtatggaat catattcgat atgacagtac ctatacctat
    43021 atctttatca tatcttctgg caaaggccaa tataactgga aaatacggaa aaagtggact
    43081 tattggattt gttgaagcat ctccaatacg ataagctatt tgagttaatg ctggattaaa
    43141 gtctagtagc ataaacatgg gaacaaatat aggtgctaaa atcgcccatt ttgcagaagc
    43201 acttcctaca aataaattga taaatccaga tagcaatata aatcctatta tcaatccata
    43261 gctaggtatc cccgactctg ctagtaattt tgctcctgtt attgataaaa ttgttcctaa
    43321 gttactaaga ttaaataaat taataaactg ggatgctata aatgctagga ctatatatcc
    43381 acccatatca ctcatactac tacccatcat agatactaaa tctttgtcac tttttatctt
    43441 cttacttact tttccataca ctaatcctgg agttaaaaat ataatagtga ttataggaac
    43501 tatacctttc attaaaggtg cattactaga tagtatagaa ccagtctctg gatctttcat
    43561 aaatgcattg tcacctatac ataaggcaac tatcactact actacaacta acaatgataa
    43621 taaagcgtat tttataccct tattttcaag ttcactcaat tcctgtatat ccttatcatt
    43681 tgttgaatca ttatattttg taaaacgagg agctataaat ttttcagtta caaatgctcc
    43741 tgcaaaagtt aaaactatcg ctgatataaa agcaaaataa agattcattg ctggattgcc
    43801 ttggtaagat gggtccatca tttgagctgc tggtattgta aatccagcta acaatacatc
    43861 tgtagtacta agcataacat ttgctgcaaa tccccctgca acacctgcaa aagctgcaaa
    43921 catacctaca agaggatgtc ttccaattcc taaaaacaca agtgctgcaa gtggtggtaa
    43981 tattataaat cctacatctg ctgctgtatg tgataacata cctgcaaata cgattgctac
    44041 agttacaagt ttttgtggaa ctttacttat accctttttc attaacactt ccataaatcc
    44101 agatttctca gcaacacctg ctccaagcat tgtaaccagt actaatccta aaggagcaaa
    44161 tgactggaag tttgtaacta tgctacctaa atatgattga atctgctctt tctctagtag
    44221 atttactacc tttacaactt cttttgtaga aggatgtatt tcttctactc ctaaattaga
    44281 tataactgct gacaatatta caacaattat acatagtaat aaaaatatgg ttactgggtc
    44341 tggaagttta tttcctgctc tctctacaac atttagtgcc ctcatcaata atccactatc
    44401 tttgtttttt tcctgtgaat taattttcct caaactaata ccccctcata aactttatct
    44461 ccagaatgct ctatcttaaa ttttattttt taatttatcc atataattta taacagactg
    44521 acatagtaca tttgtaccta ccaatataga atttttatca gctttaaatt taggattatg
    44581 ccactgatat atacaatctt gttctttatt gcctacccct aaccagtaaa aaaatcctgg
    44641 ggccttttcc ataaatatag aaaaatcttc tcctcctgta gaaggtattg gatcaactat
    44701 agcatcttct gaatacaaat cacacacaga cttacaagct attgtgtaca tctctttact
    44761 atttataaca gctggtaaat caaataaata atctagcttt gcagttgctt gatatccacg
    44821 agctatctca caaactatat tttctaaatt ttctgatatt ttctttcgta cttcattact
    44881 aaagcttcta catgtacctg tcatatctac cttttctgaa attacattat ttgcagtccc
    44941 tccatttata gtacctattg atataacagc tgattctaat ggacttatgt ttctgcttac
    45001 tatggtttga atacccatta taattgcact tgatgctact atagggtcta ttgttctgtt
    45061 tggtatacct ccgtgacctc caaatccatt tacttctatc ttaatagtgt ctactgctgc
    45121 cattaaccca cctaacttaa cacctatttt tccacaaggt atatctggat gattatgaag
    45181 tccaaatata gcatcaactt ttggattttc cattactcct cgttccacta gcatcttcgc
    45241 acctttattg acttcttcgg ctggttgaaa tataaacttt acatttccat gaagactatc
    45301 tctaatatga gacaatacat atgcacatcc tacaaggcaa gatgtgtgta tatcatgccc
    45361 acatgcatgc attataccat catatctgga tttatactca atatcaacct cttcatatat
    45421 tggcaatgca tcaatatctc ctctcagtgc tactgtggga ccatcatact tcccttttaa
    45481 taatccaaca actcctgttt ccattcctaa atctaatatt tctatgtcta aacttactaa
    45541 taaatctttt atagcttttg tagtcctaaa ttctttaaaa cttaattcgg ggtgcatatg
    45601 aatatctgta aaaaaatcta aaatatcttc tttatacttt tctgagatta aaataagttc
    45661 atgcatacta acacctctcc aaaattttat ttttttattt tttata
//
LOCUS       DAMKPG010000023        45199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMKPG010000023 DAMKPG010000000
VERSION     DAMKPG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.025"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            161..391
                     /locus_tag="PC621_001357"
     CDS             161..391
                     /locus_tag="PC621_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108520.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            448..1098
                     /locus_tag="PC621_001358"
     CDS             448..1098
                     /locus_tag="PC621_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF4108521.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            1312..1983
                     /locus_tag="PC621_001359"
     CDS             1312..1983
                     /locus_tag="PC621_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108522.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1980..3110
                     /locus_tag="PC621_001360"
     CDS             1980..3110
                     /locus_tag="PC621_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4108523.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3515..4360
                     /locus_tag="PC621_001361"
     CDS             3515..4360
                     /locus_tag="PC621_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108524.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4360..5388
                     /locus_tag="PC621_001362"
     CDS             4360..5388
                     /locus_tag="PC621_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108525.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5391..6404
                     /locus_tag="PC621_001363"
     CDS             5391..6404
                     /locus_tag="PC621_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108526.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6886..8136)
                     /gene="cme"
                     /locus_tag="PC621_001364"
     CDS             complement(6886..8136)
                     /gene="cme"
                     /locus_tag="PC621_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF4108527.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8222..8968)
                     /locus_tag="PC621_001365"
     CDS             complement(8222..8968)
                     /locus_tag="PC621_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4108528.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9083..10105)
                     /locus_tag="PC621_001366"
     CDS             complement(9083..10105)
                     /locus_tag="PC621_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF4108529.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10105..12525)
                     /locus_tag="PC621_001367"
     CDS             complement(10105..12525)
                     /locus_tag="PC621_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF4108530.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12611..13531)
                     /locus_tag="PC621_001368"
     CDS             complement(12611..13531)
                     /locus_tag="PC621_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4108531.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
     gene            14141..15067
                     /locus_tag="PC621_001369"
     CDS             14141..15067
                     /locus_tag="PC621_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF4108532.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NAEIVTKNSDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            complement(15255..15875)
                     /locus_tag="PC621_001370"
     CDS             complement(15255..15875)
                     /locus_tag="PC621_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108533.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(15847..17430)
                     /locus_tag="PC621_001371"
     CDS             complement(15847..17430)
                     /locus_tag="PC621_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4108534.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            complement(17574..19601)
                     /locus_tag="PC621_001372"
     CDS             complement(17574..19601)
                     /locus_tag="PC621_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4108535.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            complement(19614..20363)
                     /locus_tag="PC621_001373"
     CDS             complement(19614..20363)
                     /locus_tag="PC621_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108536.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            complement(20660..22312)
                     /locus_tag="PC621_001374"
     CDS             complement(20660..22312)
                     /locus_tag="PC621_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF4108537.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            22911..24032
                     /locus_tag="PC621_001375"
     CDS             22911..24032
                     /locus_tag="PC621_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF4108538.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            24068..24481
                     /locus_tag="PC621_001376"
     CDS             24068..24481
                     /locus_tag="PC621_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4108539.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            24474..25013
                     /locus_tag="PC621_001377"
     CDS             24474..25013
                     /locus_tag="PC621_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4108540.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(25213..25698)
                     /locus_tag="PC621_001378"
     CDS             complement(25213..25698)
                     /locus_tag="PC621_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108541.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            26549..26926
                     /locus_tag="PC621_001379"
     CDS             26549..26926
                     /locus_tag="PC621_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF4108542.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            27334..29139
                     /locus_tag="PC621_001380"
     CDS             27334..29139
                     /locus_tag="PC621_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108543.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            29216..29755
                     /locus_tag="PC621_001381"
     CDS             29216..29755
                     /locus_tag="PC621_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HDF4108544.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            30378..31592
                     /gene="dpaL"
                     /locus_tag="PC621_001382"
     CDS             30378..31592
                     /gene="dpaL"
                     /locus_tag="PC621_001382"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF4108545.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            31925..33112
                     /locus_tag="PC621_001383"
     CDS             31925..33112
                     /locus_tag="PC621_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF4108546.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            33128..34711
                     /locus_tag="PC621_001384"
     CDS             33128..34711
                     /locus_tag="PC621_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF4108547.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            34739..36067
                     /gene="ssnA"
                     /locus_tag="PC621_001385"
     CDS             34739..36067
                     /gene="ssnA"
                     /locus_tag="PC621_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF4108548.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            36266..37618
                     /locus_tag="PC621_001386"
     CDS             36266..37618
                     /locus_tag="PC621_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF4108549.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            37810..38919
                     /locus_tag="PC621_001387"
     CDS             37810..38919
                     /locus_tag="PC621_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4108550.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            39202..40593
                     /gene="hydA"
                     /locus_tag="PC621_001388"
     CDS             39202..40593
                     /gene="hydA"
                     /locus_tag="PC621_001388"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF4108551.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            40587..43148
                     /gene="xdh"
                     /locus_tag="PC621_001389"
     CDS             40587..43148
                     /gene="xdh"
                     /locus_tag="PC621_001389"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF4108552.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            43300..44646
                     /gene="rpoN"
                     /locus_tag="PC621_001390"
     CDS             43300..44646
                     /gene="rpoN"
                     /locus_tag="PC621_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF4108553.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT        17070 a         5518 c         7649 g        14962 t
ORIGIN      
        1 aaatatgacg aataaaaatg ataaatataa taatctatat atttacatta ggagttttct
       61 atactataat tatttatatt taaaactaaa tataaatgtt ggaattattt aggatttgaa
      121 attgtctaaa gataaatata agtgatgaag aggtgatgtg atgagattag atatggatag
      181 tatgttatat gtactaattt atatgacatt attggtatct atcattggaa gtatatttta
      241 tttttgtaaa gacctcttaa atcataaaat taagtatgtt ttaataagag acatatttgt
      301 aaatagaatt acatattctg aagaacggtt gcctaaacag tatatagttt ttcagaaata
      361 tgatatttgg aggtattgta gtttgtttta atttgaaatg aatctattat aaattctaaa
      421 gtttaaaaat aaaaaggaga gaataaaatg gatttcattt caagtatatt gcaaggatta
      481 ttaagtatat tatttaattt tacaggtgat tttggaattt ctattgtact tataacttta
      541 atagttaaat tagtacttat gccactatca ttaaaacaaa gattttctgt aaaaaaacag
      601 caagaattag ctgaaaaaat ggaacatata aaagaaaagt ataagaatga tactaaagaa
      661 ttggaaaagc aaatacaagt aagttctgta gaaagtatga agagtatgct gggatgtctt
      721 atagttttgg ttcaaatgcc agtagtatat gctttatatc atgtatgttt agatatgaca
      781 aaggaattta caacaattat aattccatgg attgctaatt taggtacatt tgataatttg
      841 tttatattac cttgtatata tacattaatt atgttagctt caaatttagt gaattatata
      901 ccatatttaa aaataaattc tcaagtaaag tttaacaaac aaatggctgt ggtgactacc
      961 ataacaagtc ttatactaac tgttagaact ccagtagcca taggtctgta ctttataaca
     1021 agtgcaattt attcattaat tgaagatatt tgttttagaa tgtattttaa tcgaaaaaat
     1081 agaatagtga caaaataata taattattat tagaaatatt ttagcctgct aacatagaaa
     1141 aagttagtag gctattttta tctatgatat caattccatt aagaatagtc taaaaaatac
     1201 tatcaaaatg ctataaaaaa tactataaaa atactataaa aaaatagttg aaaaataaat
     1261 aaattaatat aaactaaaat aaagcctaca aaaaagaggt gataaataga aatggctaaa
     1321 atactagttg tagaagatga aaaaaggatg caaagtatta ttgtagaata tatgcaaaag
     1381 ggaggatata cttgtataac tgctaatgat ggagtcgagg cacttacaat attaaagagc
     1441 aacaatatag atttaatgat attggatatt atgatgccgt atttagatgg attttcagtg
     1501 tgtagagttt cacgtgaaat gactaatata cctattatca tattgacagc taaaggtgaa
     1561 gaggaggaca aattaaaggg ttatgaatat ggcgcagatg attatataac aaaaccattt
     1621 agcccaaaag tacttttagc aaaagttaat gccttgttac gtagatacac tactgatatt
     1681 cccaaaaatt cattgagttt aggaaagatt tttattatgg tagcatcaag gcaagtgtat
     1741 gtagaagata aattgattga tttaacatat aaagaatttg aattattgcg tttatttatg
     1801 gaaaacccta atcaagtttt ttccagagag aagcttttaa attgtatatg gggatatgat
     1861 tttgaaggaa acacacgaac tgtagataca catataaaaa ccttacgcaa aaagcttggt
     1921 agtgaaggac accatatagt tacattaatt cgttcaggat ataagtttga ggttaaggaa
     1981 tgaatagttt aagaaagatt atagaaaaac tgaaaaatgt agatatttca aattggatta
     2041 aacaaaattt tggactatat tcagttagaa aaaaagtgtt tttactttct aaattatcag
     2101 gagcgttaat tatattattt tatctggttg ttgaagaatt ttcgataaac tctaaatttg
     2161 aattttggat ttggtttata atactggtta tatttattat gagtattgat tttttattgg
     2221 gaaaatttat ttcagaacca atcacatcta taaataaatc tgcaaaaagt atgtcccaac
     2281 ttgatttttc taatccatgt actgtaaata ctaatgatga atttggagaa ctttctagga
     2341 gcctgaatac aatgtctaca aatctacaac aggcattatc agacctagaa agtgcaaata
     2401 ttcaacttga aaaggatgta aataaggaaa gaatgctatt ggaacaaaga aaggagttgg
     2461 tagatgcaat ttcacatgaa atgaagactc cacttggcat tatacgtgcg tatacagagg
     2521 ggcttatgga tgaagttgat gaagaaaaaa agaaaaatta tatgaatgtt ataatagaag
     2581 aaactgatag aatgaacaat atgattgtat cattattaga cttatctgca ttggaagcag
     2641 gagtttctaa acttaaccca gagcgttttg attttataga gtttgttgaa actgtggcag
     2701 gtcgtctttt aattgatata ccagatgcca atttttattt tacatatgat ttgccagagt
     2761 atgaagtttt tgtagtggca gataaaatga gaatggaaca agtagtagag aatctaatta
     2821 ttaatgcaaa gaagcatgtt gtatataatg gtaatttaga tttatcgatt acatgtgaaa
     2881 atggcttgct tttatttgaa atttataatg atggtaggat tatagagcaa gatgagattt
     2941 caaagatttg gtctaagttt tatcgtagtg ttcaatctca gcgtaaagga ggttcaggtc
     3001 tagggcttgc aattgtttca caaatactta caatgcaagg gttagaatat ggagtgaata
     3061 atcgtgatag aggagtagag ttttatttta tgattccaat tgatgaataa tttagtatta
     3121 atttataaat tataagtatc tgatgtaaaa tatttttctt tgtatacgta agtatttaaa
     3181 gagaataaaa taaaaaactt aatgaaaaag tctaataaaa agtttaatga aaagtcttgt
     3241 tttcctgatg aaaacaagat tttttattaa gctttttatt atttcacttc gatttcacaa
     3301 gagagacact ctgatttcac caaatagaaa taaaataggt taaataaatt aaaaaataga
     3361 agcgtaatta aataaggtta caagggggag tacttatgaa atggtataca tggttgacta
     3421 ttatattgat aatattgctt tctattccgc ttaaaattaa atttataaaa tggtgggata
     3481 gacgacaaaa aaatgataaa ggaggttatg atagatgata aaagtaggag agttaaaatt
     3541 ttcttatgga aaagataaac agatattgca tggacttaat tttgaagtaa aagaaggaga
     3601 aatctttgga ttccttggac ctaatggttc aggaaaatcc acaacacaga aaatcttaaa
     3661 tggcgtttta aaaggttaca atgggcacgt atcccttttt gggaaggaag ttaaagcata
     3721 tacagagtct ctatatcaaa agattggtgt tttatttgaa tttccttatt tatataccaa
     3781 tttaagtgca attgataatt tagaatattt ttcgtctttt tatcctaaaa atcaaaggag
     3841 agacatatat gaactcttag atttgttaga gtttaagaaa gagtttataa ataagcctgt
     3901 atcatcctac tcaaaaggta tgaagcaacg tattagcatg gcaagagcat tggtaagtaa
     3961 tccaaagttg ttatttttag atgagcctac tagtggactt gacccatctg gagctgtact
     4021 atttcgtaag attattgaag aagaacgaaa aaaaggaaca actatctttt tgactactca
     4081 taatatgcta gatgcagatt taatgtgtaa caatgtggct tttattgcag atggcaagat
     4141 tatggtaata gataaaccca aaaatttaaa aatgaaaaat agcaataata aagttgaagt
     4201 ggaatttgta tataatggaa atcgagatat tgaaattgta gatatacaag agttagaatc
     4261 tggaataact tttaaatatg atgaaatctt aagtgtacat tcaaaagaac ctacactaga
     4321 agaagttttt attaaataca caggaaggat gttagtgtaa tgtattctaa attttttacc
     4381 ttagtaaaaa aagatttttg gatgatgatt tatggtaaat tttttgtgct gacacttggc
     4441 tctctcatac tttattcgtg ttatattaat tttggttatg taaagtttat ggatgaagat
     4501 agttatcaca ataatgtata tctatataat ccagagaata taagcatgag tgcatctcca
     4561 ttaattaatc aagtttcttc tataggtgaa ttaaaagcaa aacttgatga agatacaagt
     4621 gcagtttgta ttgatatgag ttccagtaag ccgaaagtaa ttttacatga aagtattaaa
     4681 ggagttgaca attatagagc tgactatgca ttgtcattat tatcatcaga aaaagaccat
     4741 aatagcaaaa tatcagacaa caatgcaata attcaaatta atgataagga tacatctgag
     4801 ataaaacaaa gaaaagacat gacttgtgag attttattct ttgaaattgt agctattgga
     4861 tttttaggga ttgcctcttt attatttaaa gaaaagcaga tgggagtaat gcgtattact
     4921 ggtattttac ctgtaaaaag aagtcttttt atattatcaa agatatttgc ttttttgata
     4981 tgtgacctat gttttacagt tcttttagta gtatttaata ttggattttt tgaaggagta
     5041 aaaatacttc cacaagtatt agttcaagct agtattttat caattattat gtctttgatt
     5101 ggaataggat gctcaatgtt attgagagat tttaagcaat tttctcttat atatcttgtg
     5161 gttgctgtat tcataactac acctatattt ctatctgcaa atacatcttt tgaaatagat
     5221 tggattaaat atcatccctt ctatcatgtt tatagtcagc ttaaaaatgc gtttttcatg
     5281 aatttttcaa aagaattagt ttattttgta gtgtgttctt tgacaataat agttctattt
     5341 ttacttgtaa aatatatttt taataaagag ttggttaggg agggataatc ttgataggat
     5401 tacgttacca gttaaagagt ataaaaaaag ataaaatgtg tataatttcc ttctttttac
     5461 cagttgttat ggcgatttta ataaatttta tgggaacaat tgatatatct tcattaggag
     5521 agatacgttt tggtatagtg aaggatacac ttcctgtaca aacagagcag tggttgaaga
     5581 agtatggtat ggtaagtgta tatgacacaa aaatgcattt aatacgtgaa attaaggata
     5641 ttaaaacgga cacaattgga gttgttagag atggtaaagg aataaaaact atgctttatg
     5701 gaaatgagat taatgctata aaaaatgttg gaaagacatt gccagagatg tttagactaa
     5761 agggtgattt aaaagatgtt gaggttacaa tattgcctaa agatagtatt ttaaaagagt
     5821 ttcagaatgt ttttattgct atgattttaa ttactgctat gtttatggga tgtaccttta
     5881 atgcaatgaa tattatatct gaaaaagaag atggaatatc atttataaat gaagttttgc
     5941 cacagagtag aagtcagtat attatacaaa aagtattttt gggtctttta tgtgggtgtt
     6001 tatcttcaat gataactgta tttatatgtt tggatatttc aatattagat atgataatga
     6061 tgttgttact cgttgtattt tcagcattta tttcatcact tataggttta tttattggaa
     6121 agttgtcaga aggtcttatg gtaggaattg tttatattaa aatagtaatg attttattta
     6181 taggtgctcc tttgttaata tatcttctag gggttaatac cagcggtata gggaatttat
     6241 gctatataat gccatcagtt gctacattta atggtattat ggagattatt aatggtaatg
     6301 ttattattat aaaagatatt gtaattttgg ttgtacattg tgtagtatgg tttatgatat
     6361 atattatttt agataaaaaa agaaagttat cttttagtag ataactcagt ataattataa
     6421 ttgggacaat ttaataggtg atttaatata ctttgtatag tgtaatttat gtagtgcaat
     6481 ttgtatactt taatttatgt agtgtaatag ttagttttat tttataatta ttcaatataa
     6541 tttagtaaga aattcagtac aattagagct acttagtgga aaacttaata ggtacttaaa
     6601 ctatataaat actttatttt cctccatttg tactataaat ttaatttatg atataaatgg
     6661 aggtattatt atatgaggta ttattatata aagttaataa agatagtatc aaaatattat
     6721 ttgctataaa gagagggact caaaataaag ataaacctat ttttcaatgt tgaaatgata
     6781 aaatatattt gatttcatat aaaaaatttt cttcataaat taaaaagtcc attttaagcc
     6841 ccctcatttt taaccagata aatcgagtta aactatttaa atatattact cggattcatt
     6901 tgacttagat attccttgct tgtaaaattg ttttgaatag gggatagcca aagcaaaaaa
     6961 taaaagtata atccctgcta cgattattaa attattaata gaaattacat ctgccattgg
     7021 tccgaaaatc atcataccaa gaggaataaa accagtatat ataataatag gtaagctaag
     7081 aactcgacct agcatttgtg gtgctacatg ctcttgcaac atagtcatag ctgatgcttg
     7141 aataattgga attgaaaaat tcgtaaaaaa cataaatata aaaaattgcc aagcttgtgt
     7201 tacaagacct agaataattg aaaaaatagc ataggataat attgctagaa aaaaggtctt
     7261 atttctattt ttaaaaccac caaaaactcc tataatcaat ccacctaaaa ccataactaa
     7321 aaatccaatc atttcaataa tagacaggta tatataaccc ttgccaaatg ttcgttcaat
     7381 aagaagtcct ataagaaatc ctgatggaac acaaagaagc atgaatgctc cataagtgaa
     7441 aagcactttt ctaatgaact tatctgaaaa acagtacttt atccctgata ccatatctgc
     7501 aaaaaatgag actttttcag tttgttttgg tatattatgt ttaggaattt tgatgaatat
     7561 caataataca atagcaataa ttgcagtaaa tacatcaatt aaaagaactt gatttatttt
     7621 attaccaaat ccaagtattg cagcagctac aataggcact ataaattgta ctatagactg
     7681 tagactacta tttataccat taaaacgcat taaatgttct tctggtacaa gttgaggaat
     7741 cattgcgttt acagcaggtg tttgtacacc acttcctagt gaacgtataa cagatattac
     7801 tgatatggct attaattcac ttgttgtgcc atttgatgac atcataaata gaattagaac
     7861 taatgtagat atagcaattg ttgaatctga tacaattatg agtacctttc gattgtagta
     7921 gtctgcccat acaccagcaa aaaaagaaat tatcatttga ggtataaagg agcatacagt
     7981 taaaactgtt acccatacac ctgatgatgt ctgaagtgtg acataccata tcattgccat
     8041 ttgtacaagt gatgagccaa ataaggaaat atattgactt gctaaaaaaa gaatgacttt
     8101 tttcttccat gtagaattag tatttttgtt ttccatccta ttcctccttt gatttgtgtg
     8161 ttgaattttt cttgctctta aggataacac acaaatcaaa gtctggagtt agagtggagg
     8221 gctaggaaaa ttgttttttc ataatttgaa gatgagattt catggttttt gcattatgtt
     8281 cagcttcttt caatgaagtt atcagtttgt cacacactga gataatttcg tcatcatctt
     8341 ttggagtgtc aaggacagtt ttaatattag tattaggatt agaagataaa gcatttaaca
     8401 ttcgtaaaat agctgaaaga gaatagtttg cacaacgaag tgcgcttata attttaagtc
     8461 gttttatatc ttcatcagta tatacacgat aaccattttg ttttcttttt acttttaaca
     8521 aaccattcat ttcccaattt cttaaagtat ctattgtaac atttaagtgt tctgcagttt
     8581 cttttctagt taaaaagagt ttatttgttt ctggagtatt tccatttaaa atctgtgcaa
     8641 caatagtaag tgcttcctcg gcattagatt tttctatttc taattgttgc aagtattgat
     8701 ttacaagact aatagcctta tcaaagtccc tattcgctga tgttcttaca atctcaaggg
     8761 catcttttct taatcccctt tgtaccactt caattttcaa ggctgtacga gcaaatctaa
     8821 attgttctat atgaaaatca ttaaagatac gataaccatt ggtattacgc ttaggtttag
     8881 gtattaagtt tagttcttca tataaacgta ctgtattagg atgtacacca attaaatctg
     8941 ctacctctga tgtcgtgtaa gtgtccatta gtattcaact ccttttagat atatagtatt
     9001 agtcataata acaccaaaca aaagtctggt gttatagtgg agggtttggt tatctaagag
     9061 taattagttt atctaagagt gattaatcta ttatagaagt aagtatatat ttttcaataa
     9121 actcccatct atcacgaggt ttaaacacta ttttagatgg tatggctatt actaagtcat
     9181 attgtggaat acaacaaata gcatttcctc cagttcccat tgctgaatac cacaaaacac
     9241 catctttttt acatatccac cacagataac cgtattcatt actattcatt ttaaccgatt
     9301 cttcaatcca cgatttagat ataatacgtt tattattcca aataccatca tttaaataca
     9361 gaaaaccaaa tcgcaacata tctctagcac tcaatgtaag tccccagcca cctatagaat
     9421 aaccctgtct atcttttgcc caacccttaa gattttttcc aaagaaatct tcaaaaccaa
     9481 aatctttcat atcataatct ggtataactt tcataccaat aggtttaaat aagtactcat
     9541 tagcaaattc acgagcagat tttccagtag tttttgttat aatagctgaa agtaggtgcg
     9601 ttccagctga agaatatttg aaagtaccaa tttcgccttt ttgaccaagt tgattcaagg
     9661 tatattttat ccaatctttt tgtttgcaaa gtctttctaa agactcagtc atatttttaa
     9721 aagggtatgg tgcagtcatt gtaagtaggt gacgtatagt tactgctttt ttacatattt
     9781 cagaaggact acaagtatat tcagggaaaa aatcaacaac cttttggtca acatctttta
     9841 tatatccttt atctattgca atacctatta aggctgatat aatacttttt gtaactgatg
     9901 caacatgaaa agtatcatca gcattatatc cattatagta cttttcaaag acaacttttc
     9961 cttttcttgc aataattatt ccatttatat tactatattg tgagattatt aaaggttcca
    10021 gttgtgagag cttttctgaa ctcatgccaa cacttgaagg gtctactttt tcaaatatta
    10081 aatctaagca attgttttta ttcattaaag atacctccct gttatagatt ttggattttc
    10141 ttttagagtt tttggagaac cttctgcaat tatataacct cctttgtcac caccttctgg
    10201 accaagctca acaatccagt cacaaacatt taatacatct atatcatgtt caacaacaat
    10261 tacagaattt ccattttcca ccaattcatc taataattga atcaacttag aagtatcata
    10321 tagacttaga ccagtagttg gctcatccaa tacataaagg atattaccat ttcgtttttt
    10381 accaatttct tttgcaagtt tgattctttg ggattcacca ccacttaaag tcggtgtagg
    10441 ttggcctaat tttatatacc ccatacctat ttgttctaat acatttaagt gtgaaacaat
    10501 atttggaata tccttgaaga aatgtacaga ttcagaaaca ctcatattta aaatatcatg
    10561 tatattttta tttttatatt taatagataa agattcatca ttaaatcttt ttccctgaca
    10621 ttcactgcat atatggtcta tacttagatt atttcctaac cagacctttt catatccact
    10681 accactacat ttaggacaag cacctttgga gttaaatgag aaatgaccag ctgtaaatcc
    10741 tcgttctaac gattctggct gacctgcaaa tagtgtacgt atcttatccc atataccaat
    10801 ataagaagca ggagttgagt tcatattttt gccaatagga gattgagata tctctgaata
    10861 tccagaaata tgtttagtac caatcagtct atctgcaata gtttcaataa ctgcataatc
    10921 gtcttcatca cctatttcgc tatctgaaga atttatttga ttattgatag tatggttatt
    10981 aaattggctt ctaagaagag gaagtagggt gtctgatata agtgaactct tgccactacc
    11041 tgattttcca gatattccaa ccatagcacc caaaggtaat gatacagtta catttttaag
    11101 attatttgta tttgcatttt gaatagttat acaaggtatt ttatcagtat gtgtaatatt
    11161 tttattgctt gttctattag gcattggata ttttccagat agatactgcc cagtaagtga
    11221 tttgtcggat tgaagtagac cttctaaatc tccttgatat actaattgac caccttcaac
    11281 accagctttt ggaccaatat caataatatg ttctgccatt tcaattgtgt ttttatcgtg
    11341 ctcaactact atgaccgtat tgcctaaatc tttaagtttc tttattgatt ttaagatgtc
    11401 tattttttct gattcatgca atccagctgt tggttcatca agcacataaa ttaatgagtc
    11461 catctctgaa tcgagatgtg aatttaaaaa tagtctttgc atttccccac cactcaaagt
    11521 tgacatttct cggtataagg atagatgacc taatcgtgat tctattaaat gtcgagtttt
    11581 ggataaaata tcatttacaa gatttgtacc aaatgtgttt aaattacctt ggtttattaa
    11641 gattttaagg aaatcatcaa tttctaaaat cgtcattttt ccaagttctc caatatgttt
    11701 tccgtttaaa agtactctac gtgcttcttc accaatttta aaaccattgc aatctgaaca
    11761 ggcagtcata gtataaactc catttaaatc ctcacccctt ttgtaacgat tgtgaaatac
    11821 ctttgataaa gaggaacttt ttttaccatt ttcataatga ccatagagta cttcatttct
    11881 tatttcttca ggtagttgta agaattgtat gtctaaataa tcgttgaatt ttttagaaaa
    11941 tactctcaaa tatcctggtg taatctttgc tctatctaag acttgtttta gggtatcatg
    12001 cttatcaagg actaattttt caatattaat ctcaaaatat gcaccatgtc cagagcactt
    12061 catacacatt ccatctgaag agttgtatga gaaataactt ggtcttaatc gttcttcctc
    12121 atacccacaa ttattgcaaa ttagattatc gcttacagga gtatcacaca tagtacacat
    12181 tatttgacct tctactgaat acaatagtgc aagcatgttg agaatattgg ttcttgtgcc
    12241 tactgttgaa cgaggattgc tctggcgaat aatattttgt tgaacagcta tagtgggagc
    12301 taaaccttga atattatcaa acttatcctc actatcaata cctgcaagta tacctaaaga
    12361 ttgcagatat tgtttacgac cttcctcaaa tacaatgtca aacataaggc tagacttacc
    12421 tgagccactg acacctgttg caacaacaag cttatctttg gggatagaaa catctatatt
    12481 tttaagattg tgtatctttg ctccattaat ttttatttct ttcattggtt cacctcattt
    12541 tatcaatttt atcaaaccgt aaaataggga tatattaatc cttaatacaa gaatagatta
    12601 ggtgaggtta ttaaaaaggt tctataggta aatatatatc gacaaagtgt ctattttgag
    12661 ggtgattgtg tgggttgtta agatatactt caaaagggaa ggaggcacgt agcttataat
    12721 taccatttga tagccattca ttatacataa aatcccacgc atcactatat tcagattttg
    12781 aaatatcaaa atgtcccaca agatattttc cagaagggat tatcatattg ccaagattgt
    12841 cattctcttt gacaacaatg ttatctggaa tggtcatgca aatactagtc cttaattgat
    12901 gctcctgtgt aaattgtgga ttatcatgat aaatggctaa tatttgtgtg ttttcaagtc
    12961 caagtagatt ctgctcactt gcatatgtga ataaccgttc taaaagtttt gggaaagtac
    13021 tggttagatt accatatgaa ccagtatatc tagtgtaaat agtattcata ttatcaagtt
    13081 ctagaatttc aatttcacct ttaacctctc tagtattctt acatttaggt tttgatatgc
    13141 ttctattgta ctgagaaatt ttatatggtt ctttgcaatt cttgctataa ttgtttctat
    13201 attttatagg gcttatttta tagtaatttt taaatgctct cgagaaaact gctgaatcag
    13261 taaagccgaa gtagtgtgca atatcagtaa cagtcatgtc agttcgatga ataagaaaag
    13321 cagttgcatt ttctagtttt agcctattta catattgtgc caaagtttct ttttctattg
    13381 ctttgaagat tctatgaaaa tgaaattttg aaaaacctgc tatattagaa agttcatcaa
    13441 gagaaagagg ttcatgtaaa tgttcttcta tatagtcttg cactttgcaa atacgattta
    13501 gatattcttt tttactttta gcatttgtca tatatagccc tccttattgt gtaaaatgtg
    13561 tattaattaa tttatagcaa tatagtaata aaattttatg tttttgcaat ttatgataaa
    13621 taagatagcg tttaattttt tataattgat aaaagatatt tataattaat aaaagatata
    13681 agatgcctta tatttatttt tgcatataag tatagtcttc gtaataatta gtataccata
    13741 gtgatttatt tttaattgtg tatttatata aatacacaac ttatgatatg gacgaattat
    13801 tatgcaaata agctgtaatt aaataaattg caatataaac cagttgaaac ataagttagg
    13861 gaaatattat caaagttatt atttagcata agagtataga gggaataaaa taagattaaa
    13921 aaattacata ctaaatttaa taaaattatt atattaaaaa taatattaag tttattgttt
    13981 aaataaaaaa gttaaatcta cactattaac ttattatact taaaacttta aaatgaaaat
    14041 atcaaagaat actttttaat aaaaataatc atttaataag ttatttaaaa atctaaaaac
    14101 tttacaacaa attatctatt taaattggag gagtgggaat atgagtaata gtttttatca
    14161 attaaattca gcagtattat attttaatga aaaaagagat gtaaatagaa taaggtttaa
    14221 tcactatgta atgagaaaag aaaatataca aagattaaaa ggaactctgc caagaggaca
    14281 atttatgatg acagtaagga aagctgcatc tgaccttgat gtatctatat ctacaataag
    14341 tagacttgta aatgaattta tagatttagg tatattgaga ttaataagta ggggagtaaa
    14401 gggaaattgt tgttctgtat atagttatgt ttgttctgaa aatagtaatg cagaaattgt
    14461 aacaaagaat agtgatattt tcaaaaatat agagaaatac attgatgcaa ctattaacaa
    14521 taaccaaaaa acaatgaaat ataaacatag agatgagttg ttcaagaaca gtaataccaa
    14581 cacatacagc cgtataaatc aagagagtat agacaaagat tatgatttga taattcatac
    14641 tgggtatgaa acagaaaatg agacaaagaa aaaagaatta ttaaaaaata atttaaataa
    14701 aaagaattta aataaaaata cctttgaaaa tttggatgaa gatttgactg aagattctac
    14761 ttataaaaat ccagaaagca tacatgaaat tattataaga aaattaaata gtgaatcagg
    14821 aaaaaatttt aaagctgatt tgagtataac taaggcactt atagataaaa gattaaaaga
    14881 agggtatagt ttagaagatt tttacaaggt tatagaggca aaggtaagaa gatggagata
    14941 tacagatatg gaaatatata tagaaccaac aattctgttt gggtatgaat ttgagtcgta
    15001 tttaaatgag gagagttctt tatctgcttg taattatggt gatatgtgtt atgaagatat
    15061 gtattgagat attagttgct ttttagttgt ttaaaatatg tttatatata aatataaata
    15121 gtgtattaaa atggagttgt ctcaaaatag agagaagcct cttcttaatt gtttaaatga
    15181 tgaaaatata tttgatttaa tacaaaggag agtgccttat gagctagaaa gttcattttg
    15241 agacactcac ttttttaata tactggagaa atataaccca ttttaatggc ttttgtaata
    15301 gcctcaactg atgatgaaac atctagcttg tcatatatgt gttgtaaatg atttgataat
    15361 gttctttcac ttatatacaa ttcatcagca attatttttc tttttttacc actacagata
    15421 agctgtaaaa ttcttttctc catattagtt aaatcttcaa taattacttc agtattttca
    15481 gatggaaaac aggttacccc ttgatagact tttaataatg aatcaattaa actatcaggg
    15541 ctaatattct tatttaggaa tccatagata cctatttttt tagcctcatg tttataaact
    15601 gggaggtcgt atccagtcaa gattattatt ttaatagttg aattagtctc taataaatta
    15661 taagctactt ttaagccatc acattcactt aagcctctaa ggtttatatc cattaagaca
    15721 atgtctattt ttttatcttg aatcatttct gaaagattat ggatgtcctg tgtggaataa
    15781 aactgttcga tttcttcgta ttcttctagt gcaattgaca agcttttagc aaaaagcata
    15841 tggtcatcaa ctaacaaaat atttataaga accatctcct ttcataggga ttgtaatctg
    15901 tacacaaact ccatgaggta tattatcaga aatacttatc gttcctccta gattttctac
    15961 ttgttcttta attgatataa tccctttgtg ttttattata tcaaaagtaa ttttagttat
    16021 atctaaaggt gttttaaaag atgtattttt cacaccatta tcattaatat ttaattttat
    16081 tatatttttt tctagtgcta aagctatcca tgcatggcta ccatttgaat gtttatacac
    16141 gtttgtcaat aattctttaa tcagtcgata tatcaatata tcatatggtt caactaagaa
    16201 taaagtatca gaacacttaa atgaaacaca aatgtttttt tgtggaaagg atgaagaaac
    16261 actttcaatt aaattctgat aattttcttt tactgttaaa ttttttaaaa taacaggatg
    16321 atagtcttgc atttgatttc ttatacatgt atttagattg ttaagtgttt catatataat
    16381 atcttgaact tctgggcgat atgacttact catcatgttt tttattgaaa gcaggttctg
    16441 taatatgtca tcatgtaaaa aattagaaaa ctctgttttt agttgttctt cttgttgtaa
    16501 tttttctaat gcaaaagtat acttgctatt tttaactact ttattttttt catctctaag
    16561 gttaaactcc aaaataatgt tacagaggaa aataagcata aataaaacat ttatagctat
    16621 taatattata gaaaaattcc cattggcaaa tagtacgacc aagctaatta ctaaaatact
    16681 tataaaaaga aatatagccc tttgttttat gttaaaaact gttgaaatag gaaaactatc
    16741 attttcttta agtgcaatac catgaatact cataaaaaat accaatacag ttagatagat
    16801 tccaaaatta ccaatatgat ttgatattct tagtgtaaaa aaataataag taaggaatcc
    16861 tataattgtt ataaatactg aaatcgatat aggctttttt tcgcttttta tgacaaaaac
    16921 aacccttttc aaatgataaa aaataatata taaaaagctt attatactaa caacaaactg
    16981 tattccgtac atacaagcat acgttctgtt agaaataaat gctcctacaa ttgcaccaat
    17041 gcaggtgata agcaataaaa tatctgttat cttacggaaa ttgtatccac taccttgaaa
    17101 caaaaataaa aatataaatt taatagagac atacactata actgtgctta atattttaaa
    17161 aataatagtg cctattggtg tggttacaaa tgataagagt aaataccaac tgtctagtgc
    17221 tagtaatcca caaaataatg aaataatgtt attttctcgg acattacaag aggttatata
    17281 tgtgatgtct atgagtaata atgttgaaat tattaaggga ataatgcttt ggatattcat
    17341 ataagtttca gtgcttgtag atattcccat gaagagaagt gatagaatgt agtttcctaa
    17401 aaatagtatt tttaaattaa aatttgtcaa tcttatacac ctccttatga gttaattata
    17461 ttaattttat gactaaatca aacctaagaa tctataattt taaattatat caaatactta
    17521 taaatgaagt ataccaaatc gtcacacctc taagcttgac gatttggtaa atattaattt
    17581 tttaatacaa ttttacaaca ggatttaata gtaagtatat aatatatcaa atacactaca
    17641 gaagttaaga aaattgcaat aatcacaggt atgtattgtg aaaatgaatt tccaagtaaa
    17701 ttctgcatca aagcagtctt ataaaccaag gtagcaaata tacagtccaa tataccaagt
    17761 aaaaacggaa tattaaaaaa tggcaatact tgttttttaa taattttttt aatttgtata
    17821 ttatcataac ccattttctg taaaatctta taatcagatg ttgaatctga aattgctgat
    17881 atattgttaa agtataaaat acttccaaca gcaataaaga ataataccac aagaaatccg
    17941 attaacagaa atgtagaact attaattgat aataaatcat ttatcctgtg agaatatcct
    18001 tgtaaataag ggcttccatc taaataatct gaaatattac tatataaatt ttcattattt
    18061 ttaattgatt taccattaat acttaaaatg cttgtcattg gtaatttatt ttgtgctatt
    18121 tgctcatata tactatcatt aacgataagt gttccaacgc tattggcaaa agaaatagca
    18181 ttgtttaatg atgtttccct tacagctaca ggtgtttctt taccattaat aagtaatgga
    18241 taaatcgttc cattttcact atatccatca ttatttggtt gatatttcat taaaatacat
    18301 tcattatccc ttaaaggggt aagttgtttt agtagctttg ttcgattttg tgcctttaat
    18361 aaagtataat aatcagaaaa agatatacat tcaaatcctg attctcttga aataacttct
    18421 ttagaagaac tactattaga taaattattc ttagatgtac caatttcata ttcaaatgga
    18481 aggtcttttg atgaagattt taccttataa atatttgtct gtgtaaaaat aatatcatct
    18541 gtattagacg tatattgact gacaatttgt tttacatctt ctatttgttt atttttttct
    18601 actctaaatt ctatctctga aggtgcaata cgcgaaacag atgcaatagg gtagtataag
    18661 gacagtgcca taacacttga aactgtcaaa gtagtagcag agagtaatgt aagcataatt
    18721 agagttttag agtttgaacg tatacgatag ataaagtttg gaatagctat tatcttcata
    18781 tctgtgtaga aagtacgttt atttttttta ctcttttgca taatatatgg aagaaaggaa
    18841 ttgataaaga gaactgtacc aattacaatg ataatcatag ttatcaagcc catttgataa
    18901 aatccaatac ttacccatac tgatttagct ccacgaagaa tatctagtga taaacaatac
    18961 ccacttaaaa tcataataaa tcccataaag gctggaattt ttctgaactt taattttttt
    19021 tctgcacttt tttcatgtct tatcaatttt acaagtgaag tcttataaag aaatttacca
    19081 tttgatatag ctaagactaa aacaactaat gctataaaga taattgtttt gtttattgca
    19141 tttatattaa aaaatggtat ctgtgaatta tcaattgata aattaagtag tgctgtaatt
    19201 ccaaagacaa tacccttatg tgacaatgct cctaatacca atccagtaat aaaagctcca
    19261 ctacaaatta atacattttc aaaggtaagt agcgataata ttgtaaactt acgatagcca
    19321 agtaaagcat atatgcctaa ttcttttgta cgacgtctca agaagaatcg atttgagtat
    19381 gccatataga agatgacaaa tgccatcaaa aatatcgaaa taacattaca catagattca
    19441 actctaccat cagttgaaat tttttctagc attatcttat taacagagaa tgaagtaaat
    19501 gcgaaaaata cagtaataac aaaagagact gaaagtagat atagagaata gaatgaaaag
    19561 ttttttttaa gattctttat tgccatataa aaaatgttca ttaaattatc ctcctatctt
    19621 ttagtatcca attttgcaat ttcttgaata atcaactcat aaaatgtatg tctattattc
    19681 ggttgtttat ttaattcttt aatgatatga ccatctttaa aaatcaaaat tctatttgca
    19741 aaacttgctg cataggcatc atgtgttacc attaaaattg ttgttttcaa ctcctcattt
    19801 gcttcctgta gtgagtggag tagttcagta gctgaattag aatccaaagc tcctgttggt
    19861 tcatctgcaa agagaatact aggatgtttt actattgctc taactgctga agctctctgc
    19921 ctttgcccac cagataattc atttgggtat ttatttaatt gagaagctat gtcaaatcgt
    19981 tcagctagac tctttaccat gttttctatt ttatctggat tgagtttctg aagtgtaaga
    20041 ggtactgaaa tattctcgaa aattgtaaga ctatctagta aaaaatactc ttgaaagata
    20101 aagcctaaat tatccttacg aaaatcagca gcacaagtgt cattaagcga tttcaaattt
    20161 atattattta tgaaaatatc tccacttgtt ggtttatcaa ttgtagataa tacattaagc
    20221 agagttgttt tcccagagcc agaagctccc attataccta taaattcacc ttctgataca
    20281 ttaaaggata ccttatctaa actcggctgt tgccctttat tataagtttt agttatgtta
    20341 tttactttta atattgtatt cattgtatat gtctcctttc gtcttaatat agtcaaatta
    20401 tatattaacc ttattgtttc ttcatgagta aatcacgcaa taattctaca caaattactt
    20461 atatcacaat atattgttta aaatctaact ttattaggtt tgggatgatg gacaagattt
    20521 taatttaaaa gagtgcatag atatataatg gcataatgta gatttaaatt aaaaaaaata
    20581 aaataaagcc ataaatattg aaaaattcaa tatttatggc tttattgctt gtaataatta
    20641 atagaatata tattttttat taatcatcta tttcaaattc taatatttta ccagtaattg
    20701 cattaattgt tatctcatat tcaatttttc cttttattat ttcaccttca tattctttgt
    20761 catcttcatc atacttaaat tttactattt tcccaccagg cactttatct agcattattg
    20821 atttagcttt ttcagcacta ataatagaac tgttgtcatt agtattagag ttattcttat
    20881 cattatcatc aaagtcatca tcagaatcat tgtccatgtc aatatctaca attcttccat
    20941 caaatgcact tatctctaat tcatatgttt tcttattttt tattacttta gcctcatatt
    21001 cgttatcttc agcatcatat ttacaataga ttaaattacc attaggaaca gcatctaatg
    21061 ctatagactt agctttatcc aatgatatat tttgtgtacc taaattatta tcatttaagt
    21121 catcatcttt ttcaatttct attattttac cagagtaggc atcaatttct atctcatata
    21181 ggatattatc ctttataact ttgcagtcat attcattatc atcttcatca taataacaaa
    21241 ctaaaatatc gcttcctgga agagcttgtt ttaatatttc tttagcctgt attaaagata
    21301 tttttttagt tttatttctt atgttcacat tttttagttt taaaatatct tcaaatgctt
    21361 tttgattata tcctcctcct acttgtgtta tttcatctat atctttattt ttaataaatt
    21421 ccttttgact atttacaagg ctaccattag atatcattat aggagaattt tctttagcag
    21481 ctaatactcc tattgataat gaatctacta atgcattttc ttggttaata ccatctttag
    21541 ctacaaatac attatttatt tttttatctt tataaaattt atcaataatt tttgcattag
    21601 tattgtttct atctgaacca ctgattcttt ctctgtttgg atatttcttt actgtattgt
    21661 ctgataaaat cttatttcct ccaactatgt aagatttttt tatagaccta ttgtttaaga
    21721 aatttatact actatcattg ctgttttcat ctgatactaa tattataggc atattatgta
    21781 ttgctgctgg agaagctata ctaactgcat cagataatcc ctttagtcca tttacaactg
    21841 caacattttc tacctttttt atcttatcta tttctttaag tacatttgta gacattttat
    21901 atatattgtt tcctactatt ttatttattt ttatgttaag tgattttatt tcattttcta
    21961 ctttagaaga aatgctattt ccactatcta caatgtacac attatctaca ccaagccttt
    22021 taagctcagc cttagttgaa ttttcaatac tgttgttgtt tactaaaagt ataggtgcat
    22081 tctttagctt agcaaatgga tttgcacaaa gcgcactttg aatagaatta ccattaacta
    22141 taatggctgt ttttgattga acccatccca atttacttac ttctactgct gtttcatatc
    22201 ttgttttacc tattaatctt tcttgctttg tagtattgtc tgcaaaaatt gctgaagaac
    22261 tagaaaaaaa catcatacct gatattaata tagctaaatt tttattattc atatttttac
    22321 ctcacaatct ttaaaaattt gttatttagc atacttaata aaatataatc ttaaaatcaa
    22381 attgatactg agtttgaagt tacaaaatca acaaataaga tttctttaat acaaaatttt
    22441 cctttttata tttaacattg tatcacaaat ataaaaagga aaaaactttt taaaaagggt
    22501 atacactatt cattttttat cttataaata agtctttacc actgttttat atattttaaa
    22561 atagacacaa gacctatttc taaatctttt ataaatattt taggtgaaat ttctttttaa
    22621 acatatctaa aaagtatttg tagtaatatt aatggactga atttcttaaa gtatatattt
    22681 ctttgcatgt tggagtttgt gaagtttgag aattggaggg tgaaagaaac atgcaatgga
    22741 ttaatattaa agaactgtat ttttataata tttgtcttaa aaataaaaaa atctattgac
    22801 ttgaagttaa ctccaagtag taaagtataa ttatacaaga attatatact tgtaagaata
    22861 gttaaagtag agagaaaaaa caaacaagtt ttaaaggggg aagtaaaaca atggaggcaa
    22921 aaaaattagg atttggatta atgcgtttac caataaaaga tgaaaatgat gttacaacta
    22981 ttgatatgga acatttaaat aaaatggtag acactttttt agaaagggga tttacatatt
    23041 ttgatacagc ctatgtgtat cacatgggaa aaagtgagat agcacttaga gaatctctag
    23101 taaaaagaca caaaagagag agttttacta tagctacaaa acttccatta atggctttaa
    23161 agaaaaaaga agagcaagaa accattttta atgagcaatt agaaaaatgt ggtgtagact
    23221 attttgacta ctatctttta cacaatatag gcgtgtcaca ttatgaagta gctaaaaaat
    23281 ttgatagctt taagtttatt gaagaaaaga aaaaagaagg aaaaataaag aatattggtt
    23341 tttcttttca tgatagtgca gaactattgg ataaagtctt gagtgagcat ccagaagtag
    23401 acttcgtgca attacagatt aattatttag attgggataa tgaaagcata caatctagaa
    23461 aatgctacga ggtggcagag aagcataata agcctgtaat tgttatggag ccagtaaaag
    23521 gaggtacact tgcaaagata ccagaaaaag cagagaaatt attgaatgac tacaatccag
    23581 atatgtcaat ttcatcttgg gcaatacgtt ttgcagcaag taaagataat gttatgatgg
    23641 tcttaagtgg gatgtcaaat atggaacaat tattagacaa tacaggatat atgcaaaatt
    23701 tcaaaccatt cgtacaagag gaatataata ttatagatga agcagtagag attatcaacg
    23761 aatcaatttt agttccttgt actgcctgtc agtattgtgt agaaggctgt ccaaaaaata
    23821 tcgctattcc aaactatttt gcgctgtaca atgcagaaaa acaagctctt aatactggat
    23881 tttcaacaca gatggtttac tataataatt atactaaaac ttatggaaag gcctcagatt
    23941 gtattgaatg taaacaatgt gaagaaagct gtcctcaaca cattaaaatt atagatgctt
    24001 taaagaatgt agcagaaacc tttgagaaat aattcaatct atacaatatc aagaggagaa
    24061 gatatgtatg acgatttcag aagttagtaa aaaatacgag ctttcagcgg atactttacg
    24121 atattatgaa cgaattggtt taataccacc tgtaaataga aataaaagtg gaattaggtc
    24181 ttttacagaa gaagattgtg agtgggttaa tttcataaag tgtatgagag gtgcaggtct
    24241 ttctattgag acattaattg agtatgtagc tatgtttcaa caagggagca gtactatcaa
    24301 agctagaaaa gaacttttaa tagagcagag aaaccaacta gcaaaaagaa tagaagaaat
    24361 gcaaaaaact ttggaacgat taaattttaa gattgataga tatgaagaag gtattattga
    24421 gaaagaaaaa gtattaaaga gcagtagaaa taagaaaatg gaggcagtat cttatgagta
    24481 aaaatgtatt aatagtatca gctagtccta gaaaaggtgg aaattctgac ttattgtgtg
    24541 accagtttat acaaggggta ttagaatcta aaaatcaagt ggagaagata tttttaaaag
    24601 ataaagatat taactattgt actggatgtg gtgcttgtta tgaaaaagaa gcaagttgtt
    24661 cacagacaga tgacatggga gaaattcttg aaaaaatgat tggggcagat gtgattgttt
    24721 tagcgactcc cgtttatttt tatactatga atgcgcaaat gaaaactatg attgatagaa
    24781 cttgctctag atatactgaa atttgtgata aagatttcta ttttatcgtt gcagcagcag
    24841 atgaaaatgt ggctgatatg gaaagaacaa tagaaggatt tagaggattt acatcatgtc
    24901 taaatggttc taaagaaaaa ggtatagtat atggtgtagg tgcttggaat gtaggtgata
    24961 ttaagggaag taaggcaatg aaacaggcat atgaaatggg taagttggct tagtatgttt
    25021 gtataaaatt gtataaaatc gaatatattc ttatcatttc aacaataaaa agaggcttat
    25081 ctatattttg agacagcctc tttgtgctat atcatttatt tttattctat atccttatta
    25141 taccctttat ttttttatgc ctatttaagt atctgttgtt actcatatat tttaataatt
    25201 acccttaaaa aactatacag attcctgcat aatattattt tctttagtaa ttaatttttc
    25261 taggtttttc caatccattt cttccatatt tgcaaaataa tgaccacttc ctatgcaagt
    25321 cagataagac tttccatcta aatcaaacga agaacctcca atacactttg aattacattt
    25381 cttacaagta cgagttttag ttatactatc tttaatagtt ttagagcatt cctccacagc
    25441 atctttatat gtatttagat gaaaaagatt tgctttaact ctccattttt tttcattaca
    25501 ttcaatagta aataagctac gttttggttt cttagttgaa tatactattt tgtaagaacg
    25561 agcacctgtt tttggttcaa ttttacaatc taatgattta ccaagttcat gtaaatgaca
    25621 tgcaatatca taatctttac cacttagtac ttctaacatt tcttctgttg tttctgccgt
    25681 taattcactt ttgctcatat taaaaaaatc cccctatata agtaataatt tcacatgaaa
    25741 ataattaata taagtatatt aaataaaact attaaaatca ataaaattac tacaatctta
    25801 cattattggg catagaatga gagctaatga tttattagaa ataagactta atagtatgaa
    25861 aatttactta gacgtggtac aattctataa aagatttaaa actaggaaag gagaaagttg
    25921 ttgagatacg gccaatataa caatttattt ataagttgta aatttattat gagattaatt
    25981 agattgttaa gtaatactat caaaaggtag ttttttggat tttcagcaac agtggtagat
    26041 attgataatt ttttggttat tattatattt agctgttata ttttcaatag gctctatgat
    26101 aactagggta aataaggtat gggtaaaggt tttattattt ataatagtgg gattgtcaac
    26161 tatagttctt tcatatgtaa taacttgata atttaagatt gaaaaataac tttaatctgt
    26221 aaattctcaa aacaagaatt ctcatgttgg taaatttcta gatatgagaa aaaaattgac
    26281 atcataaaaa agtaatttta aagcattttt ttacgaaaaa attcagaagt aactattttg
    26341 aatgacttca caaaatttta tatataaata aagtttggca cgttaattgc tttaataaaa
    26401 gttaacaaga cacaaataaa cataatcatc taagtattga ttatttgaag ttttagattt
    26461 gaaatttaat ctaaaggttc gtattaatat tggtgtttag gaaattttat tttcctgtaa
    26521 atataactta ataataggag gtgaagtcat gtcaaaagta gtaatagcta cagagaaagc
    26581 tccaggagca attggaccat attctcaagc aattcaagtt ggaaacatag tttctacatc
    26641 aggtcaactt ccaatagata tgaagactgg cgagttagta tctgacccaa aagaagcaac
    26701 aaaagcttct ttagataatg taaaagcaat attagaacaa gctggtacaa gtatggataa
    26761 aatatttaag actacagtat tcgtaaaaga tttaaaggat tttaatgata ttaatgaagt
    26821 atatgctaca tattttaaag aaaatccacc agcaaggtca tgcgtacaag ttgctcaatt
    26881 accaaaagat gcagttttag aaattgaagc aatggcagta ttataagtat tataacaata
    26941 aaaccaataa caaaactaat aaaaattaaa aactaataaa aaaagtctac aattaaaaaa
    27001 gtttatatta aaaaagtcta tataaaaatc tatctaaata tttaactatt gagcttttct
    27061 catataatgt atatttttat gtcaaaaata tatttgttat atgaggaaag ctttttttat
    27121 tttattattg tattgtgatt tactttattg tctgtatctt ataattaagt aattaaggtc
    27181 atcatataaa acaaactttt tataaattaa tattgtgtgt tttaatgtaa atatataaat
    27241 aagtaatgtt ttatatatat tttaaagagt gactatatgt taaaatatag ctaaactgat
    27301 aatttggtaa attaaattct aatgggggaa actatgttaa gtaaattaaa agaatttcaa
    27361 caagaaatga taaaatacac tgaaacagta gctagtgtgt tagatgtaga tattgaaata
    27421 gttgatgata gattaataag aattagtggt acaggtctgt ataaatcaaa gataaatgaa
    27481 agcgttgtta cagaaggatt tatttacgac aatgttatac agacaggtca ggaattagtc
    27541 gtattggata tatgtgataa tcagctttgt atagaatgtt cccattacat gaaatgccta
    27601 aataaagtaa taatagcagt tccaataaag tataataatc gaactatagg agtaattggt
    27661 gctattagta cagataaaac taaaaaagta gaaataagtg caaaaataga taattattta
    27721 aaatttgtca atcatatatg cgatttaatt tctatgaaaa tagaagaaca tgaagcgtca
    27781 aaaaatagca gtagaaaaat ggatatgatg atagaaatca ttgaaaatgt tgaaaaaggc
    27841 gttataatac tagacataaa tagcaaaata tcttatataa acaatatagc tctaaaaaaa
    27901 ttagatatat ataaaaatat aatagaaaat atagtaaata tagtatctgt tgaatcttcc
    27961 tcaaatggac atgaattact agaaattgat attgataaca aaatttataa tataaatgcc
    28021 aaaataattc cggtatatcc atatataaat caatacgaca aaataataat atttgacaag
    28081 acatatatca atcataaagg acatggaaaa gtcaatagtg gctggggaaa tagtgatata
    28141 gagtctataa ttggaaattc agaggcaatg ttaaaagtaa aagagagaac taagaaatta
    28201 gcaaagtcaa attcaactgt acttataact ggggaaagtg gtacgggtaa agagctaatt
    28261 gctagggcta tacatgctga aggtagcaga tggaacaagc catttattgc gataaactgt
    28321 gctgctattc cagaaaatct ccttgaaagt gagctatttg gctatataaa aggtgcattt
    28381 agtggagcta gtagtggagg taaagtaggt aaatttgaac ttgcaaatga aggtgttata
    28441 tttcttgatg aaataggaga tttgtcaatg cctttacaag caaagttact tagagtttta
    28501 caagagagaa aatttgcgag aattgggtca aataaactta tagatttaga tataagagtg
    28561 attgctgcta caaataaaaa cttattgaaa ttagtaaatg aaggtaagtt tagggatgat
    28621 ttatactata gattaaatgt aattcctata aatttgccac ctcttaggga aagaaaggat
    28681 gatattgagg caataatgat gaagtttgct tctaaatatt ctttagaatt aggtattcag
    28741 ttaaataaaa tagaagaaaa tgtaatgaat atgttgatta actataattg gccgggaaat
    28801 ataagggaac tggaaaatgc agttgaatat atgatgaatt tggttggaga tgatggaata
    28861 atatataaag atatgcttcc attggatatt ttaaattatt ataatattaa tggaaatgtt
    28921 tgtaaaaata aggatataaa tataatattt gaagatgaca tagttggagg gatagtagag
    28981 aatcaggaaa ggatactttc aattaaagaa ttagagttga cttacataaa caagttgttg
    29041 aataagtatg gaagagatac aaagacgaag aagaagatag caaaggattt aggtataggt
    29101 ttggctacac tatatagaaa attggaagag gaacagtagt aatataatat taaaatgata
    29161 ggtatttgtg tgtttttata gataggtatt tttactttat ggtggaggtg ttattatgca
    29221 atatgcgata gagctatatt atgataaaaa aacggaaaag caactttttg atttatctaa
    29281 aaaaatcgct gatgaaaaaa ttagtacgaa gtacttagaa tggaaaacaa gacctcatct
    29341 tactttggct tgtttcaatg atgtggatga ggcttgttgc atagacaagc taaaggggtt
    29401 tgcacaaaac cataaggtaa tacctgctta cattggttca gtaggcatgt ttaatgatac
    29461 taaaacggtg tttgtatcac ctataatgaa tagcagtatg tatcagtttc agagagaatt
    29521 atatgaggta ctacaagatt ttgattcaaa tggttgggaa tggtaccacc ccaatcgttg
    29581 ggtaccccat tgcacaatag cacttactgg agatgatgaa agtgaggctt tttttcgggc
    29641 aagtgatgta ttactgcgtg agttcaaaaa aataagtggt gagtttgtct caataggatt
    29701 ggttaaaatt acatttcctg tggaagaaat ttttacagtg aatatgaata ggtaacttta
    29761 actctgttgt ggatgttatg aatattttgc atagtgcatt tcatcagata aatgcgaagt
    29821 tgcgaatttc gacagctcaa tagtcgcaag taggacacaa tggtacacat attgcacttt
    29881 tgcttattgg agggacgccc atgtaaactt ctgtaagaag ctatgaaaag atttaagcca
    29941 ttttggaggc tttgggtaat aaagaatatt tgggtaataa agaatataaa gtatataaat
    30001 ttatagagtt tatgcgtaag aaggttatag ccatatattt atagaaaata aaagtagtaa
    30061 tatatctgac aaaacaatag acatataaaa aataagagca taataaagga agaattaaaa
    30121 taaataaacg atttctcgaa ttgataattc tcgtattgat aaaaatttcc caatatgaga
    30181 attttttctt ttataaatct tatttttcac atttaatatt tataaaattc aaagaaattt
    30241 tataaatata tgtaattcca acataaaaaa attgttttta gaataaataa tattggcata
    30301 gtaattgctt cactataaga cacaaagata aaaaattaaa atgacaccga aaaatttgaa
    30361 aggggaatat aaaataaatg agagaaataa aatggaaatt aaataactta cctaaatcag
    30421 aggaaaaaga aaaaggaatt gagtttttaa gagatgaaga aattgcaaag gctaaagctt
    30481 ttcatgagag ctttccacaa tatgaaaaaa ctcctttagt taaattagat aacttagcaa
    30541 aatcactagg cgtaagcgga atatatgtta aagacgaatc ttatagattt ggtttaaacg
    30601 catttaaagt tttaggtggt tcttactcta tgggtagata cttagctcaa agattagata
    30661 cagatatatc tgagttaggg tatgacaaat taacttcaaa agaaataaaa gaaaaattag
    30721 gagaaataac tttctttaca gccacagatg gaaatcatgg tagaggagtt gcttggacag
    30781 ctaataaatt aggtcaaaaa tctgttgtat taatgccaaa aggttcttct gaatttagat
    30841 taaataaaat caaaggagaa ggtgcagatg ctagtataac agacttaaac tatgatgatg
    30901 cagtaagatt agctaatgat tatgcagaag ctgatgacca tggagtaatg gttcaagata
    30961 cagcatggga cggatatgaa gaaataccag catggataat gcaaggatat ggaactatgg
    31021 ctaaagaagc tatagaacaa ttaaaagaat atggagtaga tagaccaaca cacgtatttg
    31081 ttcaagcagg agttggttca cttgctggtg cagttcaagg gtatgttgct tcaatatatg
    31141 atgaatgccc aataacagta gtagtagagg ctgatgaggc tgattgttac tataaatcag
    31201 cagaagctgg agatggaaaa ccaagatttg ttggaggaga tatgccaact ataatggcag
    31261 ggcttgcttg tggagaacca aacactatag gttttgaagt gttaaaaaat tattcatcag
    31321 cattcgtttc agcaccagac tgggtatctg ctaaaggtat gagaatatta ggaaaccctc
    31381 taagaggaga tgagaaagta atctctggag aatctggagc agttacaact ggagcattag
    31441 tttcaatcct tgaaagtgaa gatttaaaag acttaagaga agctttgaaa ctagatgaga
    31501 actctaaagt attattaata agtacagaag gagatactga ccctgataaa tatagagata
    31561 tagtttggaa tggtgaatgc caaagtaaat aaaagactta aggaaaataa aaacttaaca
    31621 aataaatgaa aaatttaagg aaaataaaaa cttaaataag tgaaaacttt aaataaaata
    31681 aaaactaaaa caaatgaaaa atctaaataa atgaaaaagt aaagcaaatg aaaaattcaa
    31741 aataaaaact aaataaaatt aaataaaacc aaataaaatt aaaataaagt ctaaataaaa
    31801 atttaataaa taaaatataa agaaataaaa aatcaataga ttaagtgaaa tgctaataaa
    31861 ttaaaaaagt gaaatatcaa aataaaacgt caacaaatta aaaattatat gggggaatat
    31921 caaaatgtta aatgaaacaa gaaaaaaaca attaactgaa gtatgccaag acttaataag
    31981 aaatgctagt tattcaggtc aagaagaaaa cgtagtaaaa gctatagaag aaaattttgc
    32041 tttactaggt tttgatagtt ggtcaagaga tagatatgga aatatagtag gatgtataaa
    32101 aggaaataaa ccaggtaaaa aaatattatt tgacgggcat atagatacag ttccagttcc
    32161 agatgcaagt aaatggagtg ttcctccttt tgaagcgaga atagtagatg ggaaaatata
    32221 tggtagagga acttctgata tgaaggggca agtaagtgct atgatggctg ctgtttcata
    32281 ctttgcagaa gatactaaca aagactttga aggtgaattg tatgtagcag gtgttgttca
    32341 tgaagaaata tttgaaggtg tttcagctag agagataagt aaggctgttc aacctgatta
    32401 tgttgtaata ggtgaatctt cagaattaaa cttaaagata ggtcaaagag gtagaggaga
    32461 aatagtagta gaaacttttg gtaaaccagc tcactctgct aatccagaaa aaggtataaa
    32521 tgcagtttat aaaatggcta atgtaatcca aaagatacaa caattagtac ctccaactca
    32581 tccagtttta ggtgatggaa tattagtttt aacagatata aaatcttctc catatccagg
    32641 agcatcagta gtgccagatt attgcaaagc tacttttgat agaagattat tagttggaga
    32701 aacaagagaa ggcgtattag ctccaataca agctttatta gatgaaatga tgaaagaaga
    32761 cccagaatta aatgcaaaag tttcttatgc agttgaaaaa gctgattgct atacaggaaa
    32821 tactatagaa agtgaaagat tcttcccagg ttggttatat gatgaagaag atgaatttgt
    32881 tcaagcagcg tataatggat taaaagaagc tggaatagat tcagaaataa ctcaatactc
    32941 attctgtacg aatggaagtc actacgcagg tgaggctgga ataagaacta taggatttgg
    33001 tccttctaag gaaaatcttg ctcatacaat agatgagtat atagaattag accaattatt
    33061 caaaggtgca gaaggatatt atggtatatt aaaatcagtt tataaaaaat aattaggaga
    33121 tggttttgtg agaactttaa taaaacatgg gcttatagta gatggaaata aaacaccagc
    33181 atatgaagga gatattttaa tagaaaacga gaaaatatta aaaatatcac aagatttaaa
    33241 cgaagaagct gacaaagtta tagatgcaaa aggtagagtt atatgtccag gtttcataga
    33301 tactcatagc cattcagacc tagttatttt agttaatcca tataatgagg taaagattag
    33361 acagggaatt acaacagaag tgttgggcca agatggtata tctatggccc ctctacctca
    33421 agagcatata tcttcttgga gaaaaaattt agcaggtcta gatggtgaaa gtgatgaaat
    33481 tgattggaaa tatgaaacaa cagagaatta tttaaaaatg atggattaca atggggtagg
    33541 tttaaatgaa acttatttag tacctcatgg aaacgttaga atggaagcta tgggacttga
    33601 agatagacct gctactaaag aagaaattca aaaaatgtgt gaaatcacag agagagaatt
    33661 aaaagcagga gctataggat tgtctactgg acttatatat ataccttgtg catactcatt
    33721 gactgaagaa attatagaga tgtgtaaggt agtagcaaaa tatgacggag tatttgtagt
    33781 tcaccaaaga agtgaagcag atacaatttt aacttcaatg gaagaaatca tagaaattgg
    33841 aaaacaatca ggagtaaaag ttcatttttc acattttaag gtttgtggta aagcaaactg
    33901 gaagtatata ccacaagtta tagagctttt agaaaaagca gaaaaggaag gaataagagt
    33961 atcttttgac caatatccat atgcagcagg aagtacaatg cttggagtag ttttacctcc
    34021 ttgggcacat tctggaggaa ctgataagtt aatagaaaga ttaagcgatg aaaacgaaag
    34081 agcaaaaatg aaaaaagata tagcaaatgg aatagaaggc tgggataatt ttatagaatt
    34141 tgctggaata gaccaaatat ttgtaacaag cgtaaaaaca gaaaagaata aagatacaat
    34201 tggtaagagc ttattagaaa ttggaaaaat gagaggaaaa gacccattag atgcaacgtt
    34261 tgatttatta aaagaagaag aaaatgcagt tgggatggta gatttctatg gattagaaga
    34321 acatataata ggattcatga aaagagacga gcaaaatgtg tgtacagatg gtcttttagc
    34381 aggtaaacca catccaagag cttatggttc tttccctaaa atactaggaa gatatgttag
    34441 agagttgaat gtactaacaa ttgaagaagc tgtttataaa atgactaaaa aggcagctac
    34501 aagctttagt ataaaagata gaggcgaatt aaaagaagga tattttgcag atatagttat
    34561 atttgacaag gatacagtta gtggttgtga tgattttatc aactctatgc agtaccctac
    34621 aggtatagat tacgtaatta taaatggaaa ttgtgttgtt gaagaaggaa aatacaataa
    34681 aattaaagca ggaaaagtac ttaaaaatta aatcttagct ttaaggagga ataaatttat
    34741 gattttagtg gggaatggga ggctaataag ccaagattct ttaaatccct atatggaaga
    34801 tggatgtgta gtaataaatg ataatctaat tgaagatata ggtactacag aagaaatgaa
    34861 agctaaatat ccagtacatg aatttataga tgcaggcaag aaaataatca tgccgggtct
    34921 aataaattca catatgcata tatacagctc atttgctaga ggaatggcag ttccaggaaa
    34981 accttctgag aactttttag agatactaaa taatttatgg tggagacttg acaaacagct
    35041 tacactggaa gatacaaaat acagtgcata tgcaacgtat atagaatgca taaaaaatgg
    35101 tgtaacaaca gtttatgacc atcatgcaag tccaaatgca attgaaggca gtttgtttac
    35161 aatatcagat gttgcaaagg atttgggaat tagaacgtgt ctttgttatg aagtgtctga
    35221 tagagatggt ggaaatacaa tccaacaagg tatcaatgaa aacgtaaact ttataaaata
    35281 cgctcaaaaa gatgatacag acatgataaa aggaatgttt gggcttcatg ctgcatttac
    35341 actatctgat gatacattaa ataaatgtgc ttcagagatg gctggtcttg atacaggtta
    35401 ccatgtacat gttgctgaag gactagcaga cttacaatat aatgtagata aatatggaaa
    35461 aagggtttta gaaagattca atgattttgg agtactaaaa gaaaaaacaa tagcagttca
    35521 ttgtatatat ataagtgcag aagaaatcag tcttttaaat aaaacaaata catttgtagt
    35581 tcataatcca gaatctaata tgggaaatgc agttggatgt tctcctgcaa tagagttatt
    35641 aaaaagggga gttacagtag gattaggaac tgatggatat actagcgata tgtttgaatc
    35701 attaaaagtt gccaatataa ttcacaaaca tcacttaaaa gacccaagag ttggatttga
    35761 agaatcacaa acaatgttat ttgataacaa tagaaaaata gctaaagctt attataaaaa
    35821 tgacctagga gtacttaaaa aaggtgctta tgcagatgtt atagtagttg attatatacc
    35881 acatactcca ttgacagaaa atacaatagg gggacacact atttttggaa tgagtggaag
    35941 aagcgtagat acaactatta taaatggaaa agtacttatg aaagatagaa agttaataaa
    36001 catagatgaa gaaagtattc tagcaaaaag tagagaatta agtcaaaatc tatggaatag
    36061 aatttaaata attgattatg caaaattcta tgaagcagga accctgtgac tttagtcatg
    36121 ggaggttcag attaatatac aagtaagaaa caagaattta gatgttaaaa acagagctta
    36181 aacaaaaata ggaatttaaa tgaagtatat aaataaaaaa tatacggaat agaatattaa
    36241 attaaatata gaagggagac ccaaaatgtc tcaagataaa agccctaaaa tagctccagt
    36301 agatgaaagg ttacctgtat ccaaatcttg ggtattttca ctacagcacg ttttagcaat
    36361 gtgtgcagga gcagtagcag ttcctatgat gataggaagt gctgcacgac tagatcatgc
    36421 tagtattgtt tttttaataa atgcttgctt gtttatggca ggtataggaa cactcataca
    36481 aagtgttggt ataggaaata ttgtaggtgc taaaatacca gtaatagaag gaactagttt
    36541 tgccgctgtg gcaggtatga cagcaatagc ctcatcatat agtgattcac aagttgcaat
    36601 acagacagta tttggctctg taatagtagc tggattgttt tgtatattac ttgctccagt
    36661 atttgaaaaa ttaataagat ttttccctag tgtagttact ggaacagttg ttatggtaat
    36721 aggtatttca ctatttccag ttgggataaa atggataact gacaacaaat tggaagcggt
    36781 tgaaccaaac tcattagcat tagctttagc agtattggca ataactttac tttcatttaa
    36841 attcttaaaa ggaatttgga atagcacagc tatattggta ggtatagtac ttggtacact
    36901 actttccatg gcagttggaa tggcagattt tagtgcagta aatggagttg gttggcttaa
    36961 cttaaatgta cctttaaaat ttggaatgcc tagatttgaa ccatctgcaa tactatcatt
    37021 gtgtttgatt atgttggtac ttatgacgga atcagttggt aatatgatag ctattcatga
    37081 aatggtagac aaagaagtaa caggtaaaaa tattaaaaga ggtctatttg gagatggaat
    37141 atctacagca ttatctggaa tattcaattc atttccaata actccatttg gtcaaaatac
    37201 aggaatagta ggtcttacag gtataaaatc aagatttata gcagtttacg caggggttat
    37261 attgctagta cttggattct taccaaaatt tgcagcaata ataggagcaa tacctaagcc
    37321 tgttctcgga ggcgtaggat ttgcaatgtt tggaatggta atggttggag gagtaaaaac
    37381 tttaagcaaa gtgaaatttg atggaactaa aaatggggtt atagtagcag ttagtgttgg
    37441 tttgtctatg ataccactaa caaatcccga tttctatcat aatttcccaa cttgggtaca
    37501 aacaatattc catagtggaa taacaacagg tagtttatca gcagtgcttt taaacatatt
    37561 cttcaatgaa ctaggttcta aaaaagaaaa aaataataaa gttgaagaag caaaataaaa
    37621 ataaaacaag taaaaatcta tattgactta aagtcattaa gcattaataa aaataattca
    37681 gagatattaa taattcaaca aaactatctt atattttaaa taatataaaa aagtaaataa
    37741 ataacgaata caaataaaag aatacaatct aaatattaaa aatatttaat ttaagggaga
    37801 gataataata tggctaattt aaaagttaat ttatatggaa tggaattttt aaacccaatt
    37861 atgacagcgg caggacctgg agcaaaagat ggtgctctct gtcaagaggc agcaaaaggt
    37921 ggagcaggag ctatatgtac aaaaactata tctgtattac cagctgatgt accaagacca
    37981 tgtatggcaa aaactaatag tggtttttta aacacagaat tatggtcaga gcttccaaaa
    38041 gagcaatgga tagaggaaga atacaaaaag gtaaaagaaa cagggcttcc agtaatagta
    38101 agtatgggat attcagcaga gcaaatagct gaggttgctc ctttagtaaa gccttatgca
    38161 gatgcagttg aattatctac tcattatgta ggtacagacg ttgcaccaat agtaaatgct
    38221 ttaaaagctg caaaagctgt tttagatgta ccagtattta tgaaaatgag tcctcatact
    38281 gacattcaaa ctattgcaaa agctgttgaa gaagcaggtg cagatggact tgtaatgata
    38341 aactcatttg gaccatgtat ggcaatagat gtaaatacag gataccctat aatgggaagt
    38401 aaagctggat acggatggtt atcaggtgca ccaataagac ctctagcaat aagatgtata
    38461 tatgaagctt ctcaagttgt aaatatacca ataatcggtg ttggtggaat cacttgtgga
    38521 aaagacgtgg ctgaaatgat gatggctgga gcaagtgctg ttcaagtatg tacagaagca
    38581 atccttaaag ggccttctat ctatggaaaa attgctaaag aattaaatga atttttagat
    38641 gagaatggat atgctgatgt taatgaaata aaaggattaa ctcataagaa aatggcagct
    38701 agagggttta gaactcattc agtagctcca gtagtggatg ataacaagtg catcaaatgt
    38761 aagcaatgtg taacaagctg tgtatacgaa gctttagaag ttacagataa gttgaacata
    38821 gatgcagaca agtgttttgg atgtggatta tgtgtaacta gatgtccaaa aggagcttta
    38881 agcatctgct acaatcaagt agaagctatg aatgagtaat aattatatag aattttggta
    38941 attaacttag agtaataggt attaggataa taaaatatat cagataaaca aacacacatt
    39001 atgcattaat aatacagtct aaagttaata attttattat attgatttaa ctaaatatta
    39061 gtttagctga atattaattt aattaggtat tggtttaatc ttaatataga tttactacgt
    39121 ataagtgaac taactagatt actttaagtt aattttttca tataaaataa attgatacat
    39181 tacaagtaag gtggagtcta tatgggaatt gttttaaaag gtgggaaagt agtaacaagt
    39241 gaaaaaactt actgtgcaga tgtaagaata gaagatgaaa aaatagtcga tataggtctt
    39301 gatatatcaa aagaaaatga tgaaatagtt gatgttagtg attcttatgt tataccagga
    39361 ggtatagata cacacacaca ctttgactta gatacaggcg ttactaagac agcagataac
    39421 tttgagacag gaacaaaagc tgctatagta ggaggaacta ctactatatt ggattttgct
    39481 acacaagcta aaggatgttc tttgaaagac ggattagaag aatggcatga aaaagctaag
    39541 gataaatcct attcagatta tggctttcat atggctataa cagattggaa tgactcggtt
    39601 tgtaatgaaa tggaggatat ggtgaaagag ggagtatctt catttaaact gtatatggcg
    39661 tataaaggaa gccttcaagt tgatgatggt gtaatctttg aagcattgag aaaagcagaa
    39721 gagataggag ggattattgg tttccattgt gaaaatgggg acataatatg tgaacttgtg
    39781 gataaagcaa agtcagaaaa taatttatct ccaaaatatc atcaattaac tagaccagct
    39841 ataatggagg cagaagctac atcaagactt atgaagatag ctgaagttgc aaactcaaaa
    39901 gtatatgtag ttcacttaag ctgtaaagaa tccttagagg aggttttaaa agctaagcaa
    39961 agaggtgtgg atgtaatagt tgaaacttgt cctcaatacc ttctattaaa tgatgaatta
    40021 tatgatttag atggatttga aagtgctaag tatgtaatgt caccaccttt aagaaataaa
    40081 gaaaataatg aaatattatg gaatgctatt ttagataatc aaatagacac tataggaaca
    40141 gaccattgtt cttttaatta taagggacag aaggaacttg ggataaacga tttcagtaag
    40201 ataccaaatg gttctccagg tgtagaacat aggatgagtc ttttatacac atatggagtc
    40261 tgtaaaaata aaatatctat caacaagatg gtagaagtaa catcaactaa tgcagcaaag
    40321 ttgtttggaa tgtatcctaa taaaggaact atagaaatag gcagtgatgc agatatatta
    40381 gtcttaaatc caaacaaaaa atccaaaata gaagcaaaaa aacaattaca aaatgtagat
    40441 tatacacctt atgaagggta tgaagtggat tgtcaaatag aacatgttta tttaagagga
    40501 aaagaagtag ttagagatgg agaattaatt tgtaatactc caagtgggag atatataaat
    40561 aggcgtctaa caaagggggc tagagaatgg tagaatttat attaaatgga gagaaagttg
    40621 ttgcaaaaga aggcaaaaaa cttttagact tccttagaga agatatgaat ataacttcag
    40681 ttaaaaatgg ctgttcagaa ggtgcttgtg gaacatgtat ggttataata gatggtaaag
    40741 ctactaaagc atgtgtattt aaaactgata aattagctgg gaaaaacata acaactatag
    40801 agggattatc agagagagaa agagatgttt ttgcatattc ttttgctagt caaggagctg
    40861 tacaatgtgg attttgtatt ccaggtatgg taattagttc aaaagcactt atagatgtta
    40921 ataataatcc tactgaagat gatataaaga aagctttaaa aggtaatata tgtagatgta
    40981 cagggtatgt aaaaataatt aaagctgtcg atttagcagc aaagattttt agagaaaata
    41041 tagatgtgcc aaaagtaaac tgtaagggtc tagtaggtga aaatcttcct agagttgatg
    41101 cagttggcaa agcacttgga tattctgaat atgtagatga tatgagaatt gagggtatga
    41161 tttatggttc agcagttaga gctaagtatc ctagagcttt agttaaaaaa atagatatag
    41221 aagaagcaaa agcttatcca ggagttgttg cagttctaac agctaaagat attccaggaa
    41281 ctataaaggt agggcactta aagaaggatt gggatacttt aataccagaa ggtgaaataa
    41341 ctagatattt aggagatgga atagttttag tagcagcaga aactagagaa gcgttagaag
    41401 aggccaaaaa gctagttaag atagattatg aggaattaac accattaagt actacagcag
    41461 atgcacttag agaagatgct ccaaagatac atgagacagg gaatatactt gttaaggaac
    41521 atttagtaag aggaaacgct aaagaaaaga tagaaaattc aaaatatgtt gtaactaaaa
    41581 gatatactac acctgctaca gaacacgcat ttttagagcc tgaatgtgca gtagctggtc
    41641 cttatgaaga tgatggagta ataatctatt caacagacca aggggtgttt gccactcaac
    41701 atgaatgtgc agatatgtta gggttaccat atgagaaggt aagagttata aacaaaatgg
    41761 taggaggagg gtttggtgga aaagaagaca tgagtgttca acaccatgca gccttacttg
    41821 cataccatac aaagagattt gtaaaagtac agttaacaag aaaagaaagt attattgttc
    41881 atccaaaacg acatgccaca gaaatggaat ttacgacagc atgtgatgaa aatggatact
    41941 taacgggtat gaaggcaacg ataatatctg acacaggtgc atatgcctct cttggaggac
    42001 cagtgctaca aagactgtgt actcatgcag caggaccata caattatcaa gatattgata
    42061 taacaggaac agcagtatat actaataacc ctcctggagg agcatttaga ggatttggag
    42121 ttacacagag ttgttttgca actgaatgta atctaaattt acttgctgaa atggttggaa
    42181 tatctccttg ggagataaga tataaaaatg caataagacc tgggcaagtt ttaccaaatg
    42241 gtcaatttgc agatgaagga acagcaatgg cagagacact agaagctgta aaagaagagt
    42301 atgaaaagca tccacgtgca ggtatagctt gtgctatgaa aaatagtggg gttggagttg
    42361 gtatacctga tgttggaaga tgtagattgg tgataaaaga tggcaaagtt cacataagaa
    42421 caagtgcttc ttgtataggt caagggcttg gtactgttat gactcaagta gtttgtgaaa
    42481 ctgctagcct acttcctgaa aatatagtgc atgaagttcc agatactcat ttaacaccag
    42541 actctggtaa tactacggct tcaagacaga cagtatttac gggagaagct actaaggttg
    42601 catcaaataa gttaaaagaa gctttagact ctggcaaaac tttacttgat ttagagggag
    42661 aagagtttta tggagaatat gaaagtatta cagataaaat gggttgtgac aaagaaaatc
    42721 cagtaagtca tgttgcttat ggatatgcaa cacaagttgt aattctggat gaaaatggaa
    42781 aactagaaaa agtagtagca gcacatgatg ttggaagagc tataaatcca atcagtttag
    42841 aaggtcaaat agaaggtgga gtagttatgg gtcttggata tgctctaacc gaagattacc
    42901 cagtagttaa ctctgttcct acagctaagt ttggtacatt aggtttattt aaatctacag
    42961 atataccaga aatacaagct ttaatagttg aaaagaacaa agatttattg gcttatgggg
    43021 caaaaggtgt aggagaaatt tgtacagttc ctactgcacc agcagtacaa aatgcctatt
    43081 atgtatttga taaagaattt agaactgagc taccactaca aaatactcca tatagtaaaa
    43141 aaaagtaaca aaaaaaagaa atattaaata aaaaaataga tgaaattgta aataaaaaaa
    43201 acccttttgc ataaggtata ttttaaatga tagaatatac atatacagga gggtttttct
    43261 tttataaaat aagtttaata aaattgagag gtgagcagaa tgaattttaa taatagtcta
    43321 gaattaactc agtctcagaa gctaataatg actacacaac ttaagcaatc attatcaata
    43381 ttaaatatga gtaagttaga gttagaagaa gagataaaaa gagaagcaga gaataaccca
    43441 ttagttgaag tcgagaaaag tggggagata aattgggaag agtatataaa agacatggat
    43501 aggtcaagac gtttagatag aacagagata agctataatc ctgataatga agttaatcta
    43561 gaaaatttag ttaaatattc gtcaaattta tatgaagatt taaaatttca gataagcctg
    43621 tataaactta cagacaaaga attggaagtc tgtgagtata taattgatag tttagatgag
    43681 gatggatatt taagaactga ggaaaaagta attgttgata ccttaaagat agatgaagaa
    43741 ctttttgaaa aatgtttaat tagtgttcag cagttggaac ctagtggagt aggggcaaga
    43801 agtctttcag aatgtctgat gatacaaatg gctagtttag gaatatataa tgaaatttta
    43861 gaggaaatcg ttacaaaaga cttaaattta attgcaaata ataaatataa agagataagt
    43921 aaaaagtaca atatgtcatt acaaaaatgt gttgaactaa taaacataat taagacatta
    43981 gaccccaaac caggaagaac ttgttcagta gagaaaagtg tatacattca acctgatgta
    44041 acagtagaaa agattgatga tgaatttatt gtatatttaa atgagaaaga ttcttaccaa
    44101 ataagaatca atagttatta taaagacata ttaaaaaatt cacagtctga tgaaagtgca
    44161 aaagaattta taaaagaaag actaaattca gcaacaggac ttatgaaaaa tatagagagt
    44221 aggaaaacta cagtacttaa gattgcagaa gagatagtaa aagctcaaga tgaatttttg
    44281 agaaaaggga caaaatatat aaaacctttg aaaatgaaag acatagcaga aaaattagaa
    44341 tttcatgaat ctactataag tagaggtgta aatggtaaat atatgctaac accttttggc
    44401 gtatatgaat ttaggtactt tttcagtagc gccattgaga ctgagaataa tgaaatggca
    44461 tctagtacaa gcataaaaaa aattataaag gaaacaataa aagacgaaaa taaaaagaaa
    44521 cctttaagtg atgaccatat atctaagtta ttaaaagaaa aaggaatcaa tgttgcaaga
    44581 aggacagttg ccaaatatcg tgaagaatta ggaatcccat cttcaagtaa gagaaaagaa
    44641 tactaataag taaaagacaa atatcagtat ggtaagggga aaaggctaat taagagatga
    44701 atgtagattg ggctaaaaat tttataaagt aaaataatag aatatggaaa agtattaaaa
    44761 tgctataagg tattaaaaaa ctggggaaca ttaaaacatt aagatagcaa agaacataaa
    44821 aaactaaaga agattaaaac attaaagaac attaggcttt gaggaatatt agaactttaa
    44881 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
    44941 taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc ttataaagaa
    45001 tgattaaatt aaataagtgt ttgtaaagaa taattagata ttagaaaaag aagaatacct
    45061 agataaataa actattatta aatttaataa tcacaaaagg aaaacacgta gggtataaat
    45121 aaaaatctta actagataaa agtaaactta gttgtagata aaaataacta gaaataaaag
    45181 taactagaaa taaaattat
//
LOCUS       DAMKPG010000024        44163 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMKPG010000024 DAMKPG010000000
VERSION     DAMKPG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.026"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            328..1161
                     /locus_tag="PC621_001391"
     CDS             328..1161
                     /locus_tag="PC621_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF4108554.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1254..2186
                     /locus_tag="PC621_001392"
     CDS             1254..2186
                     /locus_tag="PC621_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF4108555.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2238..2720
                     /locus_tag="PC621_001393"
     CDS             2238..2720
                     /locus_tag="PC621_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4108556.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2732..3601
                     /locus_tag="PC621_001394"
     CDS             2732..3601
                     /locus_tag="PC621_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF4108557.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3644..4399
                     /locus_tag="PC621_001395"
     CDS             3644..4399
                     /locus_tag="PC621_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF4108558.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            4441..5262
                     /locus_tag="PC621_001396"
     CDS             4441..5262
                     /locus_tag="PC621_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HDF4108559.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5267..6028
                     /locus_tag="PC621_001397"
     CDS             5267..6028
                     /locus_tag="PC621_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF4108560.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6189..6446
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6559..6798
                     /locus_tag="PC621_001398"
     CDS             6559..6798
                     /locus_tag="PC621_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF4108561.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7068..8063
                     /gene="ilvC"
                     /locus_tag="PC621_001399"
     CDS             7068..8063
                     /gene="ilvC"
                     /locus_tag="PC621_001399"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF4108562.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8116..9804
                     /gene="ilvB"
                     /locus_tag="PC621_001400"
     CDS             8116..9804
                     /gene="ilvB"
                     /locus_tag="PC621_001400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF4108563.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(10162..11292)
                     /locus_tag="PC621_001401"
     CDS             complement(10162..11292)
                     /locus_tag="PC621_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF4108564.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(11371..12078)
                     /locus_tag="PC621_001402"
     CDS             complement(11371..12078)
                     /locus_tag="PC621_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF4108565.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            complement(12091..12960)
                     /locus_tag="PC621_001403"
     CDS             complement(12091..12960)
                     /locus_tag="PC621_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDF4108566.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            complement(13580..14086)
                     /locus_tag="PC621_001404"
     CDS             complement(13580..14086)
                     /locus_tag="PC621_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF4108567.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(14162..14710)
                     /locus_tag="PC621_001405"
     CDS             complement(14162..14710)
                     /locus_tag="PC621_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108568.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            complement(14722..15063)
                     /locus_tag="PC621_001406"
     CDS             complement(14722..15063)
                     /locus_tag="PC621_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF4108569.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            15240..15443
                     /locus_tag="PC621_001407"
     CDS             15240..15443
                     /locus_tag="PC621_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108570.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            15491..16300
                     /locus_tag="PC621_001408"
     CDS             15491..16300
                     /locus_tag="PC621_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDF4108571.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            16347..16556
                     /locus_tag="PC621_001409"
     CDS             16347..16556
                     /locus_tag="PC621_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108572.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(16566..16940)
                     /locus_tag="PC621_001410"
     CDS             complement(16566..16940)
                     /locus_tag="PC621_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108573.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            17016..17213
                     /locus_tag="PC621_001411"
     CDS             17016..17213
                     /locus_tag="PC621_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108574.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            17244..17636
                     /locus_tag="PC621_001412"
     CDS             17244..17636
                     /locus_tag="PC621_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108575.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            17617..17766
                     /locus_tag="PC621_001413"
     CDS             17617..17766
                     /locus_tag="PC621_001413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108576.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            17837..18274
                     /locus_tag="PC621_001414"
     CDS             17837..18274
                     /locus_tag="PC621_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108577.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            18286..19452
                     /locus_tag="PC621_001415"
     CDS             18286..19452
                     /locus_tag="PC621_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDF4108578.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            19467..19802
                     /locus_tag="PC621_001416"
     CDS             19467..19802
                     /locus_tag="PC621_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108579.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            19855..20427
                     /locus_tag="PC621_001417"
     CDS             19855..20427
                     /locus_tag="PC621_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HDF4108580.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            20450..21022
                     /locus_tag="PC621_001418"
     CDS             20450..21022
                     /locus_tag="PC621_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDF4108581.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            21022..22980
                     /locus_tag="PC621_001419"
     CDS             21022..22980
                     /locus_tag="PC621_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDF4108582.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            22992..25409
                     /locus_tag="PC621_001420"
     CDS             22992..25409
                     /locus_tag="PC621_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108583.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            25691..26017
                     /locus_tag="PC621_001421"
     CDS             25691..26017
                     /locus_tag="PC621_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDF4108584.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            25966..27336
                     /locus_tag="PC621_001422"
     CDS             25966..27336
                     /locus_tag="PC621_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4108585.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            27360..27497
                     /locus_tag="PC621_001423"
     CDS             27360..27497
                     /locus_tag="PC621_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108586.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            27490..27969
                     /locus_tag="PC621_001424"
     CDS             27490..27969
                     /locus_tag="PC621_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HDF4108587.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            28252..28801
                     /locus_tag="PC621_001425"
                     /pseudo
     CDS             28252..28801
                     /locus_tag="PC621_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            28996..29970
                     /locus_tag="PC621_001426"
     CDS             28996..29970
                     /locus_tag="PC621_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108588.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            29991..30500
                     /locus_tag="PC621_001427"
     CDS             29991..30500
                     /locus_tag="PC621_001427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108589.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            30570..31262
                     /locus_tag="PC621_001428"
     CDS             30570..31262
                     /locus_tag="PC621_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108590.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            31240..32493
                     /locus_tag="PC621_001429"
     CDS             31240..32493
                     /locus_tag="PC621_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF4108591.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            32499..33941
                     /locus_tag="PC621_001430"
     CDS             32499..33941
                     /locus_tag="PC621_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF4108592.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            33931..35466
                     /locus_tag="PC621_001431"
     CDS             33931..35466
                     /locus_tag="PC621_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF4108593.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            35468..35716
                     /locus_tag="PC621_001432"
     CDS             35468..35716
                     /locus_tag="PC621_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108594.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            35784..36347
                     /locus_tag="PC621_001433"
     CDS             35784..36347
                     /locus_tag="PC621_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF4108595.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            36359..37249
                     /locus_tag="PC621_001434"
     CDS             36359..37249
                     /locus_tag="PC621_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF4108596.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            37268..37501
                     /locus_tag="PC621_001435"
     CDS             37268..37501
                     /locus_tag="PC621_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108597.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            37511..37912
                     /locus_tag="PC621_001436"
     CDS             37511..37912
                     /locus_tag="PC621_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108598.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            37906..38253
                     /locus_tag="PC621_001437"
     CDS             37906..38253
                     /locus_tag="PC621_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108599.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            38253..38543
                     /locus_tag="PC621_001438"
     CDS             38253..38543
                     /locus_tag="PC621_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF4108600.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            38653..39459
                     /locus_tag="PC621_001439"
     CDS             38653..39459
                     /locus_tag="PC621_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108601.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            39808..40245
                     /locus_tag="PC621_001440"
     CDS             39808..40245
                     /locus_tag="PC621_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108602.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            40238..40414
                     /locus_tag="PC621_001441"
     CDS             40238..40414
                     /locus_tag="PC621_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108603.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            40415..41725
                     /locus_tag="PC621_001442"
     CDS             40415..41725
                     /locus_tag="PC621_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF4108604.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            41742..42212
                     /locus_tag="PC621_001443"
     CDS             41742..42212
                     /locus_tag="PC621_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF4108605.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            42271..43098
                     /locus_tag="PC621_001444"
     CDS             42271..43098
                     /locus_tag="PC621_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108606.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            43170..43610
                     /locus_tag="PC621_001445"
     CDS             43170..43610
                     /locus_tag="PC621_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF4108607.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            43951..44112
                     /locus_tag="PC621_001446"
     CDS             43951..44112
                     /locus_tag="PC621_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108608.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
BASE COUNT        17924 a         4566 c         7956 g        13717 t
ORIGIN      
        1 attaaaaatg tggcaaatag ttgttttggt atttattatt tgtttaatat ttctaattta
       61 taaaaaaaaa attataaatt ttaaaaatta taaatgacat tttaactaga attaattgta
      121 aaaaacacta tataatactc aattttcaac attctataca attatacaga aattatacag
      181 cataataatc accgaaataa caaattttaa aaattaagca aaactttttg tcgaagttca
      241 acgtctatat atatgtagtg taaatttttg ggataaaata ctacaaaaat ataaatgtaa
      301 aaatatagta atattggagg tcgaacgatg agaaataaaa agaaaaaaat aaatagaaag
      361 aagttgtatt tgcttttaag tgctattgta gtgtgtctgg cttttatagt agttggtgtt
      421 cgcgtcagct ctttaaatat gaaggaaaat gaaattagta gaaatgcaaa actgtcaagt
      481 tctactatta cagatatagt atctaataca tcaatagaag ttagtaaagg gccaagtaaa
      541 agttctggaa aaatagcata cataactata gatgatggcc catctaaatt tactgacgag
      601 atgataaaaa ctttaaataa atacaatgtt aaagcaactt tttttatgat agatgggaat
      661 atgaaggaat atccacagca agttaaaaat ataattaaaa atgggaacac agcaggtttt
      721 catagtgtat cacatgatat tcacaagctt tatgttacta gtacatcagc aaaagaggaa
      781 tttgatacaa atgatcaaac tttttataag ataactggta aacattcaaa agtaataaga
      841 ataccttatg gaagcaaacc atatacacca caagcctctt atcaggcttt agttgatgct
      901 ggatataaaa tttgggactg ggacttagat acagaagatt ggagatctaa ttcttctcaa
      961 attgtacaaa atgtaaaaaa tcatattaag aatagaaaag gtgaagataa agaccaactt
     1021 gtagttttaa tgcatgagaa aaaacagagt gcagaagctt tagattcagt tttaaaattc
     1081 ttatcagatg aaggttatga atttgcgcca gtagatcaaa accaaatacc aaaaaattat
     1141 tggttacgta atttagagta gttaaaatat aagaatattg attgatattt ttagaaattg
     1201 agaataaaaa tgagtgaggt gtaggtatat tgaataaaaa actttatata ggaatggtcg
     1261 gaatattgtc tttgatgatg gttggttgta ataaaagttt agcaaaagta aatgatgtgg
     1321 aaatcacaaa agaacagtac aaaaaaacaa aagcagtttt atctgctact aataattata
     1381 taaatgggca gtctttagat gaattagaaa aaactttgga taaaaaaggt agaaataaat
     1441 tagaaaatgt tataatatcg tttatggtgg ataatgaact tttatatcaa gaggctaagg
     1501 ataaaggact aactccaagt aaaagtgaag tagactcaaa atatcaagaa ctagaagata
     1561 agatgaattt gaatacaagc tataaggaaa aaatggacaa agcaggtgtt gataaagaat
     1621 atttaaaaca agaaatatct agagatttag caatagataa aaataaaaag gcctttgaag
     1681 atagaataaa cataagtgat aatgatatgg aagcctacta tacaagtcat aaaaaagatt
     1741 ttaatgtaga agaagtaagt gcttctcaga tacttatatc gactttagat aaaaataaaa
     1801 aagaagtaag caaagataaa aaagaagctt tgaagaagaa ggcagataat atattgacta
     1861 aaatcaaaaa tggagaaagt tttgaaagtt tagctaaaaa atattcggat gataaagcaa
     1921 ctggaaaaaa tggaggacag ctaggatatt ttacaaagga tgataaaaat gctgagttta
     1981 caaaggaagt gttcaaatta aagaaaaatg aagtttcaaa tgtgtttgaa actagttatg
     2041 gatatcatat agtcaaagtt actgataaaa gagaaagaca aaaaagtttt aatgagtgtc
     2101 agagtttaat aagagaatct attttaaatg aaaagtatat tgagcatata aaaaaactga
     2161 atgaagatgc taaaatagac agatagaatt tctaacagct caatcctagc ctgtaatcgg
     2221 agggagattt tacaggaatg tttaagagtt taaagagaac gaaagttgag aaatacatta
     2281 tcgataataa agactctttt tatagaatag catatagtta tacgaagaat gaagaagatg
     2341 ccttagatgt ggttcaagaa gccatgtata aggcactata ctcagtggaa aatattaaag
     2401 aagtaaatta tataaagaca tggttttata aaattttagt tagaacgagt atagatttta
     2461 ttagaaaaaa ccgtaaatat aataatatga cagatattga ccttatagat gagactggag
     2521 agtatgataa atatactgat ttggatttaa gaagagcttt ggaagaatta cctattgaat
     2581 acaaatctat tataatatta agattttttg aagatttaaa gattgaagaa gtagcaataa
     2641 tattggatga aaatgtgaat actgttaaaa ctaggttata tactgcttta aaaaaactaa
     2701 aattaaaaat tgaagagtag ggataaaaag aatgacaagc aaagaaaggt taaataagtt
     2761 aaaagatgag tatcataaaa ttccaatacc caagaaatta gatactataa taaatcatga
     2821 aaaaatagaa aacatatatc gagagaaaaa taaaaaagta aatagattaa gatttaaagt
     2881 tgcaatagcc tttgcatgta tatttacagt attagtaaat ataagtcctg tatttgcaga
     2941 caatttttct aagatacctg taattggagt aatagtagaa gtaataacta taaaaaatta
     3001 tagtttaaaa agtgaaaatt atgaagctga gattgatatt cctaaaatac gaggtttaaa
     3061 agataaaaac ttagaacaaa gattaaacag tagttttatg gaagatggaa aaaggcttta
     3121 tcatcaattc caagagagaa tggagaagat acaatcatca aagaataaag gatataaatc
     3181 tttaagtttg agttatagtg taaaaaataa ttctaaaaaa tttctatcaa tagagatgac
     3241 aaaaaatgag atagaggctt catcatatgt gagtaaggca cattatacta ttgataaaaa
     3301 aagacaaata gttttgactt tgccaatgct ttttaaagat gataagtata taaaagttat
     3361 aagtgataat ataaaagaac aaatgagaga acaaatgaaa aaggatagta caaaatccta
     3421 ttttatagat caaaaaaaag acttgcctgt tgaagatttt aaaacaatta ataagtatca
     3481 ggatttttat tttaataaaa atgaagacct tgtaatttgt tttgatgaat atgaagtagc
     3541 acctggttat atgggtgcag ttgagtttgt aataccatat aaagtaataa aagatttgta
     3601 aattgaatag tagtttatat atacatattt taagaaggac aatatgacta catataaaaa
     3661 gcttaaaaaa tctatattta aaatttctat attttcaata tgcttattag tctcattagt
     3721 agatataaac cctatatttg caggtagttc ctctaatgag acttcaaata gtgatacgat
     3781 aaaggtgtta aatgctaaga gtctcaactt taaacaaggc aattatgaag tgaatgtgag
     3841 tattcctaaa ataaaaggtt taaaagataa aactttagag aataaattaa acaatgagtt
     3901 tttgcaagat gggaaaaaaa tttacagtga atttcaaaat aaaatgaaaa aggaaaataa
     3961 taagggtcat agatacctaa atgtaatcca taatgtcaaa aataattcta aggacttttt
     4021 atcaatagaa atgacaaaag aagagataga agattcctcg tctataacta agaagcatta
     4081 cactgttgat aaaaatagag gaatactatt aaatttacca atatttttta aagatagtag
     4141 atatattaga gctataagtg ataatataaa agagcagatg agaaatcaaa ttaagaaaga
     4201 tgatacaaaa tcttatttta tagaccaaga taaagacttc ccagtgaaag attttgaatc
     4261 tataaataag tatcaagatt tttattttaa taaaaatgga aatcttgtga ttagttttga
     4321 agaatatgaa gtagtacctg gttatatggg agcagtagaa tttgtgatac caaataaagt
     4381 aataaaaaga ctaagataga tatatagaag atttttagat agactggtaa ggagaattat
     4441 atgaatatat atgaacaagc aataagatta atagaaaata atgaagattt cgcatttgct
     4501 acaataacgt ctcattcagg ttcaacacca cgtgaaactg gagcaatgat gattgtgaaa
     4561 aatgattcta ctatatttgg ttcagtagga ggaggtagtg tagaagcaga atgtataaaa
     4621 catgcaatta atgttataaa aaatagagaa tcaatgttgt ataaatttac tctaaacaaa
     4681 tctgatgtgg caaaactagg aatgatatgt ggaggtactg gagaaataca aatagatttt
     4741 atagatagta aattaaaaag taatatagaa aaattcaata agagactaaa ggaaaataca
     4801 agtaaagcat atatatttgg tgcaggtcat atttcaagag atgttgctgt gatactatct
     4861 cttttagaat ttagaacagt agtaatagat gatagagaag aatttgctaa ttatgaaagg
     4921 ttcccagaat cagaagtagt agttttagat tcttttgaaa atataccaga ttttccaact
     4981 gacgaaaata gctatataat tattttaaca agaggtcatc tatatgattc aagtgcttta
     5041 gaatgggctc ttaaaagaga aactggttat ataggtatga ttggaagtag aacaaaaatt
     5101 ggattgacgt acgaaaaact catgaaaaaa ggttttaaga aggaagaatt atcaaaagta
     5161 catgcaccaa taggcataaa attgaatgct caaacaccag ctgaaatagc tgtatgtata
     5221 gctgctgaac ttataaattg tagagctaat aaggaaaaat aaactaatga acacagggag
     5281 taaagatatt gatactttaa atgttaattt tagagggatt gaaataaaag cagttttagt
     5341 atataaaaat agaaaaaata tttctataaa aatagatcca tttggcaaaa taattgtaat
     5401 gagtcctcct aaaatatcca aaaaaataat aaaagatata attattgaaa aaggagactg
     5461 gatacttaaa aagtcagaaa aatataaagg aagagaagaa gtatacaaac aaagaatgtt
     5521 tataactggc gaaaaatttc tttatttagg tgaagagtac catctagtta taaaagaact
     5581 gttgaaagat tctgtcaaaa aaagtaactg tagaataact attaacgaat atcaaatagt
     5641 tgttcagaca aatgatacaa gtacagattt tattaaaaaa tctttaaaat cttggtataa
     5701 gatggaaagt gaaagaacag tattagagag aatagatttt cttaaattaa attgtgaaat
     5761 aatgaagcaa ttagttccag caagtgttaa ggtaaaagaa caaaataaga gatggggaag
     5821 ttgtacagct caaaaaaata tttatataaa ctcaaaaata tctatggcaa gattagatgt
     5881 aattgattat gtaataatac atgagtttag ccatttagta catatgaatc actctaaaaa
     5941 attctatgat ttagtaaaat ccattatgcc cgattataaa gataaagaaa attggctaaa
     6001 aataaatgga tataaaataa taatctgaaa acgattattc caaaatacag gcgtaaagta
     6061 taaaagaagt attattgtga aaaaaataaa taatctgaaa attttaaaaa aagtcattga
     6121 cattgtaaat gatagggaat ataatctata ttaacaacaa aacagaatta aaatataaaa
     6181 ttaaaaaatt aaaagcaatg acagagacaa caatactaat agtgttatcc acagagaacc
     6241 aatgttgctg agaattggtg ttaacagtag tataatgatc actctggagc ttttgtactg
     6301 aatattttta gtaagtacaa acgtatgtca acgttacatg actaaggtta aggtaattaa
     6361 ttacccgacc tgaaaaaaga ggggtagatg tattttttat atctacaaat aaggtggtac
     6421 cgcggaatat aactttcgtc cttataagta atatttatat tgcttataaa gatgaaagtt
     6481 tttttatttt aagcgatata aataaattta atccatataa aattaataaa aaaataataa
     6541 aaataaggag ggatttttat ggaaaatact gtatatgcta aattaaacca aggaatagat
     6601 tcttttttaa gagtggctat gactctaaga agaagagagg ttgatataca atcaataagt
     6661 atgactgtag ataatatgaa taattcaggt ataaaattaa tagtaaatga agaaaagaca
     6721 tctttagatt ctgtcttaaa ccatatgaaa aaactacatg atataagaga gataactgtt
     6781 gaaagaatga gacattaatt aaatatattt tataaagcta aagataaata ttaaataaga
     6841 aaaatcttag attaaaatat aagtgtcatt gaatttatca gtttatcaca gttaataaat
     6901 ttattagttt gttgtagcta ataaaaactg aaaattctaa aattataatt gtaagattat
     6961 ataaatttag aatttaaatc tgttgaataa tattaaaaca atgataatga gtatgaattt
     7021 ctaataaaaa taaatatcat aaaaataaga aaaagtattg ggaggaaatg aaaatggcaa
     7081 gaatgtatta tgaaaaagat gtggatttag aggttttaaa aaataaaaag gttgcagttt
     7141 taggttatgg aagtcaagga catgcacatg cacaaaatct tagagataac ggtgtacatg
     7201 tagtgatagg tctttatgac ggaagtaaat cagcacaaaa agcaaaagaa gatggatttg
     7261 aagttaagag tgtagcagaa gctacaaaag aaagtgattt gacaatgatg ttaatgcctg
     7321 atgaaaaaca aaagaaagtt tatgaagaga gtgtaaagga taacttaaaa gaaggtcaga
     7381 cattagcttt tgctcatgga ttcaatatac attataatca agtacaacca ccagaatttg
     7441 ttgatgttgt aatggtagct cctaaaggac ctggtcactt agttagaaat gtatttacaa
     7501 agggaagtgg agttcctgca ctatttgcag tatatcaaga ctacactaaa aaagctacag
     7561 agacagtttt agcatatgca aaagggatag gagcaacaag agcaggtgtt ttagaaacta
     7621 cttttaaaga agaaacagaa acagatttat ttggagaaca atcagtactt tgtggtggaa
     7681 taagtgaact tataaaattg ggatataaaa cgcttgtaga tgcaggttat caaaaagaag
     7741 tagcatactt tgaatgttta catgaaatga aactaatagt cgaccttata tatgaaggtg
     7801 gatttgaaag aatgagatat agtataagtg acacagcaga atatggagat tatgtatctg
     7861 gtaaaagggt aataactgat gctgcaaaac aaggtatgca aaatgtttta gaggatatac
     7921 aaaatggaaa atttgcaaaa gcgtggatta aagaaaatga agaaggacgt gaaaacttcc
     7981 taaaaactag agaagaagaa tataatacag aaattgccga agttggtaga aatctaagaa
     8041 gtatgatgtc attcttaaaa taaatatata ttaaatggat tagtttgagg gaaagagtgg
     8101 tgtatagtag tgcgtatgaa tggagcaaag gtaattttag aatgtttaaa gaaagaggga
     8161 attgacacaa tatttggata tcctggtgga gccgtgatac ctctttatga tgctctatac
     8221 gattattcag atgattttaa acatataagg acatcccatg aacaaggttt agttcatgct
     8281 gcagatgggt acgcaagaag tactaataca gtaggagttt gttttacaac atctggtcca
     8341 ggagcaacaa atgccataac tggaatagca acagctttta tggattcttc acctatggtt
     8401 gtaatttcag ggcaggtgcc aactagttta ttaggaaaag attcttttca agaaattgac
     8461 ataactggag caacattatc tatgacaaaa cataattatt tagttagaaa cactaaagaa
     8521 ttagttccta caataaaaga agcttttaga gttgcaaatt caggtagaaa aggacctgta
     8581 cttgtagatg ttccaaaaga tttattttta gcagaaatgg attttagtgg tgaggactat
     8641 gatttatgtc aaatagatga ttatatggat tataagagtg attttgatct agatgatgaa
     8701 actaatatta agcttttaaa tgaagcaata gatatcataa aagaatctaa aaagcctgta
     8761 atatatgcag gtggaggtgt taaatcttca gacagtgagg aaattcttga aaaatttgca
     8821 actaagatag atacacccgt actaaataca cttatggggc ttggaaatat agatagaaag
     8881 aatgaactat ctcttggaat ggttggaatg catggaagta gagaaagtaa tttagcactt
     8941 tctaattcag atttagtaat agcgataggt gcgagattta gtgatagagt gataagtaaa
     9001 agttctgaat ttgctaaaaa tgcaaaaata atacacatag acatagaccc atctgaaata
     9061 agtaaaaata tagaatctaa tgtatctttg gttggagatg taaagctagt ccttagttta
     9121 ttaattgaaa gggttgaaag taagaataat agcaattgga aagaagagat aaagacattt
     9181 agaaagagtg agggtgtaca aacatatgaa tttcatccac aaaatatact taaaaaaata
     9241 aatgaaaaat atgaaacatt aaagaaaccc actgtggtag taactgatgt cggtcaacat
     9301 caaatgtggt ctgctaaata ctggaatttt aaaggtaata aaagttttat aacatcagca
     9361 gggttaggaa ctatgggatt tggtttaggt gcagctattg gaactaaagc tggtaatgta
     9421 gataaaaatg tggttttagt aactggtgat ggaagtttta gaatgaactg taatgaacta
     9481 gctacagttg caaattataa tgtacctatg cttattttac tactcaataa tagaacatta
     9541 ggaatggtaa gacaatggca gaagttattt tccaatcaaa gatactctca aactgatatt
     9601 aatgaaaatg tggattatgt taaactcgta aatgcatata atattgatgg atataaggta
     9661 tccaatatgg aagaattagg aaaggttcta gatgtgatag attttaataa acctgtattt
     9721 ttacaatgtg atatagataa ggattatgat gtttatccta ttgtagcacc taatgatgca
     9781 ttggaaaatt tgatatgtaa ctaaaactta tactgaatat tgataaaatt ttttattaaa
     9841 tttttattgg tattcagttt ttgtttagag atagattggc attttttatt tttgcaaata
     9901 aatcaatgaa tatataatta agaacttatt aaattttaat tatacaattt agaatagtat
     9961 tacaacttaa gtaaatatta agttttattc gttagattta ttattaatac cttatattag
    10021 ttatggttgt tatatactct aaagccttga aattacaagc gtatagctga ctatataaat
    10081 ttgataattt tagcttttaa aatagataaa ttagattttg ccacccattt gccaccgtat
    10141 tatatttttg ggtggcaaac tctaatttgt aattttttca aatatatcta cagtttcatt
    10201 tttcatttta tcagttacat gtgaataggt atccattgta gttgataatt ggctatgacc
    10261 caaacggttt tgtatgtctt taatgttagc accattttct aataatagag tagcatgtgc
    10321 atgtcttaaa gaatgaaaat ggaagtcatt gtttaaagct actcgaattt gtcttactat
    10381 agtgtctaaa gtgtgagtat tcacttgttg accattttct ttggtacata cccaatcact
    10441 atcaaagtaa aattctccgt attttaattt cattttcttt tgatataatt tatgttcttt
    10501 taatgccttt attaaagtgt cacctgtaaa tatagttctg caagagcttt ctgtttttgg
    10561 ttgccctaat tcaaacattc catttggttt tttaatcaaa gtatgcttta ctgtgatagt
    10621 tttattatca aggtctatat tatcccattt tagtgcaata atttcacctc ttcgcatacc
    10681 agtatgaaat ccaattagta aaactatacg ttgaaatgaa tcttgaggaa atatatttag
    10741 tatttgatta aattcttcta atgtaatagt tttaacttta ttagtttctg ttttagattt
    10801 agtttttggt atgcttacat attgcatagg gttttctcgt atgtgtttgt aaggatggac
    10861 tgctgatttt aatgacctat gtaatatggc ttttaatact tgtaatgtat tttgagaata
    10921 atcctctttg tactttttat ttatgaagtt ttgtagtatt gcaggagtta aagcttttac
    10981 tttgtaagct cctagttttg gctttatatg tttttctatg tttattcggt agctttcttg
    11041 agtgttgtat ttacagttga gtaaaacata ttctttgtac caaaagtcta ggtagtctga
    11101 taagctgatg ttactttctt caaagactat gccagagttt tcatattcat ttagtgcttc
    11161 tcttagtgct ttttctgctt ctttttttgt attgccacca actctttcta ctttttttct
    11221 ttttccttct actatgccta ggtcaaagta gtaataccat ttgttgcttc tttttcttac
    11281 tccacctttc ataatagtat ccctcccttt tagaatgtat gtttgtttgg tgtttatata
    11341 aaaaatgtct cctatattta caagaagaca ttaattatat tcgtcaaata atatttctaa
    11401 tttaactttt atacattcat cacaattatt ttctttgtta acaaatttaa taatgcaatt
    11461 atactttaat cctctattta ttatacttgt taattctcca ctataatatc taggaatgta
    11521 acctatatga ttctcttttt tatcaaatac cttgattgca tgaggatcaa atttattttc
    11581 tggttctaac tctagaacta gagcttcatt tgattctaat tctattgaat tatcacattc
    11641 atgtccttca cacccagcat aatgtcttac tcctgcaata taaaaatatc taattatatt
    11701 tttatcaatg tccaaaggaa ttggatctac aaattctagt gtatctatag gtaatcttgc
    11761 accacttctt tttaaaagtt catatgtatc atactcagac atatcatact tatccaaaat
    11821 tttattgatg tcctttcttt ttttatcagg taatctactt gcaaatgggc taaataaatc
    11881 ttcactataa tatgttttac taatatcatc aaatgatatt aaagcattaa aacctgcttt
    11941 aatagcttca tcaatttcat attcataaga aaattcatac aatccatttt tagctaactt
    12001 tccaattata aattgtcttc tactttttgg atctttccaa ataacatata aaaaatcttt
    12061 tccattttta attgacattt tatccctctt tcacaaatta tatatatttt ttattcttaa
    12121 cactttttct tttagatata tttttagcaa tttttttaac tttgggctta taatattttc
    12181 tgaatagtta tctattaatg tatttatgtt tacttcattc ataagattta ttatcttttc
    12241 tacaaaatct atagtttgtt cataataatt tgaatttaaa tattttagca cttctaagtg
    12301 tgttggttct tttttatttt ttccatctat ccttattctt gatcttgaac gtgtatttat
    12361 aagagcttca aatctttgat gatcagtgaa gaaagaatct atgttctcaa tattaacata
    12421 acaacataat gatgatccgt tatcatatag aggactaaga gtaattttat tttgactttc
    12481 tagtagagcc caattatttt gatgcctatc tctatttcca atcaagtaat caaaaattaa
    12541 catttttaga aaatcatttt ttatgttata ttcttctata gatttaaaaa tcattttcaa
    12601 tgaatagtat tcattatttt tataatcata taacttatct gtattataac aaggataata
    12661 tttgtttatt aaccacacgc cttcaatcaa tttctcatca tttttattga ttaaataact
    12721 cattgaacca attcttttat tatatgtacc tatctcaaca ttagcacaag gaatctttaa
    12781 caaactagct atttcacagg ctaatttttc tgcccaatat tctccagttt cttttgtttt
    12841 agggaattta aacaagccta tatctttagt tttttcatta attagccaga ttttctcgct
    12901 tcttccacta ccataagaaa aaccatcata ctctttccat tcatcaaaat ttttaatcac
    12961 gaaaataatc ctctcaaaca agtattttat attcttattg tatcatttga ttctaaattt
    13021 gcaagaattt tattatccat aataagctta acttaaaata aaaatttttt ctagatttta
    13081 tttattccac ttttaacttc aagctttatt acatatggaa tatgtgttta ctcatcaaaa
    13141 ttactactgt gtataatggt tttactttat aaatacttaa ttttagtttt ctatatttat
    13201 ttggtaattt ttttgagtgt tgttaagtaa gaaatatttt ttgtaccaaa aggtaagctg
    13261 atattgcttt attcagatac tatgcctagg tcaaagtagt aataccattt gttgcttctt
    13321 tttcttactc cacctttcat aatagtatct ctccctttta gaatgtatgt atgtttggtg
    13381 tttatataaa agagcagatt aactgctctt tatatacttt tgttatgtac aatattatct
    13441 taattttgtt tatctggttt ttacttaatt attttcttaa tattcttaca attttttagc
    13501 ttttatgtgt agtttattat aataatattg aaatatatat ttattaagta aaaaatctat
    13561 aaaagtagaa taaatgagtt taaaaaatat cctcatatat gtagaaattt ggcaaattag
    13621 ttaatactaa aaatctatta tttcctaagt ccaacatgct tttctttttt gatagaaatt
    13681 ctaatctttt tagtaagaag ttgatgctaa cttgtaattc ttcagctatt tcgtatacac
    13741 ttgtagcgtg tgaattaata acatgtatta tttcttcttc tgttataaga aattcacatg
    13801 cccattttag tgctttgttt tcagttttgt ctatcaagat tttatttttg taactgtttt
    13861 ttgaagatac atagtttcca acactggtaa aatgatgtcc taattcttct gctaagattt
    13921 ctattagttt agcattgttt tgttttaatg aattaagtaa tgatataatt tttagtcctt
    13981 gtctgtttat atacaatcct tttatgtcat ctgctatttt gtcagtgtag taaatttcta
    14041 tctcttcatt atttgctaag tctaaaagtg cgtctagttt gttcattgaa atccccctat
    14101 aaaaagaatg tatgtgttac ttttttatat ataaagagca ggagtgaact gctctaaata
    14161 ttcatttttt atatctatca actaaaaact ctatgtagct attaatatgt tcttgcgctt
    14221 ctttaggtaa actttcataa gatttaatta ttttggatat ttcatcagta gttatgtaat
    14281 tttcaattga ggttttacca agtagataat caactgacac attaaaaaat tctgaaattc
    14341 ttaacaagtc ttctttaaca ggttctcttt tgctagtttc taacatagca attttgctta
    14401 gtgaacaatt aagtaatttt gataattctg cttgagttaa ttcttttttc tttcttaatt
    14461 cttttaacct tattggaaaa gttgaattta agcattttgt taatttattt gaatcatcta
    14521 tgttaatttt tccaattaag taatctgtag atactccaaa atagtctgag caatctttaa
    14581 taaatgattc ttttggttct cttaacccat tttcaattct ggataaagtg gatttattta
    14641 tatttaagtc tgtgcttaat ttatctaaag aaattccttt ttcagttctc aattttttta
    14701 atattaacat agattcaatc cttatttttt atatttattc attaaaaatt caatataatc
    14761 gttgagttgt tcttgagctt cctctggtaa atctttgtga ggattaactc tgtgtgcggc
    14821 tacagtatcg atgtgatttc taacaagagt tctacccaaa agataatcaa ttgatacatc
    14881 aaaaaaatct gcccaatttt ctagaatatt tatttctggt tttcttttat tattttcata
    14941 ttgcgatatt gttgatttat taaaacttgt aaagtaaact ttattgaatt ttgaaactag
    15001 ttcatcttga gtaagttttt tctcttctcg taattgtttg aaacgttctc caaaagttgc
    15061 cacaaacaac acctcccaat aattaaatga tactataagt ttacagaaac gtaaacttat
    15121 gtttagaaaa aaataaaaaa gttaacaaaa aaataaactt atgtattgac aagttttgtt
    15181 tgtgtgaata taataaaagt atgaagttca caaaaataaa acattggaag gtgaagaaca
    15241 tgaagaaaag aagtttaaaa gctggtcgaa tagaagcagg atatacccaa gaagaactag
    15301 catataagat aggaatagca aaatcaacat ataatctaaa agaaaatgga aaaagaaatt
    15361 ttacggaaaa agaaatgata atgattagct atattttaaa taaaacaatg gatgaacttt
    15421 ttttagaaaa aaggttaaca taaattaaaa tttattgttc acaaaaacaa tcataaggag
    15481 gaaagaaatt atgaataatt tacaaatatt caaaaataag acttttggag aaataagagt
    15541 aatcgaacta aatggagaat tttggtttgt tggaaaagat attgcagaac aattaggata
    15601 taaagataca tctgatgctt tgaaaagaca tgttgatgat gaagataaag gagtaggtga
    15661 aataccgact cctggtggta atcaaaatat gaaagtaatt aatgaaagtg gtctttattc
    15721 tttaatatta agttctaagt tgccaagtgc aaaattattt aaacgttggg taactaatga
    15781 gatattacca agtatacgta gtactgggac atataatatg atagatttgc aaactaaact
    15841 accaactaca tataaagaag cgttacaaca tcttatagag caagtagaag taaatgagca
    15901 gttacaacta gaaagtaaaa tgaaagacca agtaataaaa gaactaaaac caaaggcaga
    15961 ttatacagat atgatattaa aaaataaagg acttgtcaca ataactcaaa tagcaaaaga
    16021 ctatggaatg agtggaaaag aaatgaataa aatacttcat gaaagaggga ttcaatataa
    16081 acaaagtgga caatggcttt tatataaaca acatcaaggg aagggataca ctcattcaga
    16141 aacaatagac ataactagaa gtaatggaat gcctgatgta aaaatgacaa ctaagtggac
    16201 tcaaaaggga agattgtttt tatatgattt attgaaaaca aataacatat taccagatat
    16261 agaaaaagga tatagttatc aaacttcaat attaggttag tactttgaaa actaaataca
    16321 gaatatttta gaaaggagag attgaattgg aaaataagaa aatagaagaa atagagaaaa
    16381 gaataaccat gcttgaaaat caattgcaag cacagttatt tagtcagata acagctcaat
    16441 caaatagtga ttttatagat tatatgtacc aatgtatagt tttgtattta catgagatga
    16501 ataaaagtga aaaaagaaaa attagtgaaa ttactaaaga aagaatcaga aaataagaat
    16561 gataattaca atataaaacc tattcttcta gcaggttttg aagggtcagt tttctttact
    16621 gaagttatca taaagtttat ctgtgatgtg tgttgaagaa tttcagaata aactccattt
    16681 actatgccgt agtagtgaat tagacaaggg ttatgataac ctacttcttc aacaatcatt
    16741 aagacatctt ttccaaagtt agtcaatttt agtgcaattt catgttcatc atcaagttct
    16801 ttttcaaatt cctctataga tttaagaata attttatatt gaaattcagc atagtaaaaa
    16861 tactctgtgt tagaagtatc agagactgtc tttcttatac tttcttgtaa ttctttagat
    16921 atattgtttg aaaactgcat aatacaccat ccttttgaaa attttggaat ttattccatg
    16981 tttatattat accatgtaga actgaggtga atacaatgtt aataggcgac aatataaagc
    17041 aaatgctaag aaaaagagat ataaaacctt ataaattggc aaaagaattg aatatagatg
    17101 taagtggttt atataaatta ctgagaaata aaagttctaa tccaactata gatactctaa
    17161 taaaattagc tgattattta gatattacat tagacgaatt agttggaaga taaataatta
    17221 aaaagagggg agagaatttt cacatggata tttctgagag tatagcaaaa caatttagtg
    17281 atagtttaaa aagtttaata gaaatagaga taagtaaaca agagacagat agagttaaga
    17341 gtcaaactat tgaacaaaag gtaaaggtac tggagcctaa agatatagtt gttttaataa
    17401 aaagaggtta tccaaattat ctaataacag tagaagaagc aaggggaatt ttaaagttag
    17461 atacagcttt tatgcgtagg ttagtaagca caggtttgat aaaatcacta gttagaggtg
    17521 atggtagaaa aatttcaaga tatgaagttg atgattttat tgaaagaaat caaggtaaaa
    17581 atttggatga acttttaaaa gcagtagagt ggggggatga aattgctgag tcttgatact
    17641 aataataaga atgtagtaac tcttaaaaaa gatggaaaag ttatagcaga tatagtattt
    17701 aaagatatta aaactggtaa gaaaatatcg gttggaatat taaataaaaa agtgttggta
    17761 aaataaccaa cacacaaaaa aataaaaaat aaaatataac acaagtaaag tataacacaa
    17821 aaaggagata tttaaaatgg ttgaagttaa tgtaaatgta aaagttaaag tagaagcacc
    17881 agaatttaca aatgcactat tagtagtggc gaatgcttta ggaggtctta atctaggaaa
    17941 agcaatgcag atagaaccta taaatataac agatatgaaa gaagagaaga aagttgaagt
    18001 taaaaaagct gaaaagatta aaaaggtaga agtaaaagaa gacgttaaag aagaaatagc
    18061 agaagcaaag gaagaaatag aaaagaataa tgaaaataca actagtgaag ttaaatacac
    18121 aaaagaagag gtaagaacta aggcagcaca agtaagtaag gcaggtaaga aggataagct
    18181 taaggagtta tttggtgaat ttggagctag taagttaagt gaagtaaaag aagaagatta
    18241 ttcagctttt atgaacaaat tagaaagttt atagggggta cataaatgcc attacaacat
    18301 gcaagactta gtgcgagtgg agcccataga tggcttcact gcactcctag tataaaatta
    18361 gaagaaaact atccaccatt aactagtata tatgcagaag aaggaacagt tgcacatgaa
    18421 ttagcagaag ttaaattaat gctagagtat gaaaaaataa gtaaaaaggc atataatgca
    18481 agaattaaaa agatacagaa aagtgagtat tataactctg aaatggagga ctatatacaa
    18541 tcttatgttg agaatgtagt tgaattagta aatgatagta aagctatatg tgatgatgta
    18601 atagtgatgt tagaagaaag actcgatttt agtgagtggg ttccagaagg atttggaaca
    18661 ggagacgttg ttgtaatatc tgatggtata cttcaagtta tagacctaaa gtatggaaaa
    18721 ggtttagaag tttcagctat agagaatcct caacttagac tatatggttt aggggcatat
    18781 aatcagtttg aaatgctata cgatattgat ttaattaaaa caacaataat tcaacctaga
    18841 cttgataaca tatcaagtga agaaatagaa gttactaaat tacttacatg ggcagataat
    18901 gttaagaaga aggctcaaat ggcttttaat ggtgaaggag agtttgtaag tggcagtcat
    18961 tgtggatttt gtagagctaa aaatgattgc agaaaaagag ctgaggataa ccttaaacta
    19021 gctagaaaat atgattttgc tgacacattt gctcttaata aatatgagat agcagatatt
    19081 ttaggttttg ctaagaatat acaagattgg ttaaaggatg ttcaaagtta tgcactagaa
    19141 caagctgaaa agcatggtgt taaatatcca ggatataagc ttgtagaagg cagaagtaat
    19201 agaaagtatg tagatgagca agaagttgct aaggttttat taaattcaga ttatgatgaa
    19261 gaaaaaattt ataagccaag gaccttaaaa ggaattagtg atatggagaa agctataggt
    19321 aaaaagagtt ttgctaaact actaagtgat ttaattataa aaccagttgg aaaagctact
    19381 ttagtagtag aaagtgataa aaggtcagaa attaatagta tagattcagc gaaaaaagat
    19441 tttgaaatat aaatggggga tattacatgc cattagatgc aggtataaaa gataaattag
    19501 atgaaagcat aagtataatt ttagatttaa aggatagaaa aaagagtatc gaaaaagatt
    19561 tagattatga aaaagataat ttactagata tgttaaagag actaaatatt acagaatata
    19621 actcaactga agaaaatgca aaagttatag tgatggattt taaaagagaa agtttaataa
    19681 aggataaagt gatgtcaact ttcctcgaag taaatgacaa tcatcttgat agagtgtata
    19741 taccagaaca tataaaagtt agtccagtat gctttgtatc agtaagagca aaggataatt
    19801 aaaaatattt aattcctagt agctataaaa agatttaaaa ggggatataa ttttatgagt
    19861 aatcaagaaa taactaaact agttgaaaat aatttgaatc ttgtacattt tagtataaat
    19921 agatttttta agtcttatgt agagcaacat ccatacttat atgaagagtt ttatcaagaa
    19981 ggatgtattg ggttatataa agcagcatta aattatgata gtaacagagg caagttttct
    20041 acagtggctg tttcttacat aagaatagaa atgtttaaag ctttgcgtta tggcgaaaaa
    20101 cattatagaa actctataga aggtttagat gtaagtgtat ttgaagatag taaaagacca
    20161 ttaaaagata tacatggatt ttatgaaatt caatttaact atgatgttga ttatataaga
    20221 aaaattgcaa gaaagtcata tttaaaagat attgataaaa tagtagattg gattttacaa
    20281 gataaaaaga tatctgatat aggagagtta ttaaatgttt cttcaacagt tatttgtgga
    20341 agaattaaat tgtttagtaa gcaagttaga gatttagata aatttggaga aataacttca
    20401 aatataaaat caatatacca aatataaaaa tattaggagg aagaaaatta tgagtaattc
    20461 agtacaatca acaaaggtag taacaggaaa ggtaagatta agttattgca atatctttaa
    20521 aagcagagca atggtagaag gtgcagagcc caagtattca gtttgtattt taataccaaa
    20581 atcagataag gtgactttag gaagaataaa gaaggctatt gatgcagcta aagaacaagg
    20641 taaaacttct aaatggggtg gaaaattacc aggtaattta aaaacacctc ttcgtgatgg
    20701 agatgcagaa agagctgatg aagcagagga atatgtagga atgtattttt taaatgcaaa
    20761 tagtacacaa aagccaggaa tagttgataa agacttaaat gaaatattag ataatacaga
    20821 ggtatatagt ggttgttatg gaagagttag tataaacttt ttcccatata atagtgcagg
    20881 aaataaagga ataggttgtg gcttacaaaa tgttcaaaag ttagcagatg gagaagtact
    20941 tggaggagct agagctagtg cagaagcaga tttctcagat gactttgaat atgaagatga
    21001 agaagaggac ttcttaagtt aatgaggacc ttatcaattg atatagaaac atatagtgat
    21061 ttagatataa aaaaagttgg agtctacaga tatgtagact ctgctaattt tgagatactg
    21121 ttatttgcct atgcttttga taatgaagag gtaaaagtta ttgatttagt aaatgatgaa
    21181 gagttaccaa aagaagtaat agaagcttta aatgataata aagttataaa atcagcattt
    21241 aatgctaatt ttgaaagaac agcaataagt aaatttttaa atattaattt aaaaccaaat
    21301 gagtggtcat gtacaatgat aaaggcgtta acactaggac ttccaggaag tttagatagt
    21361 gtgtctaagg ctttaaagtt taatgaagat aaacagaaaa tgaaagaagg taaagcatta
    21421 atacaatatt tctgtaaacc ttgtaaggct acaaaagtta acaagggaag aactagaaat
    21481 ttaccaatac atgatatgga aaaatggaat aaatttaaag aatattgtaa acaagatgtt
    21541 gtagttgaaa gagaaataag aaacaaactt agtaagtata agactaccga aagagaaatt
    21601 aaattatggt atttagacca gagaattaat gatactggta ttaaagttga tacagagtta
    21661 atagagaatg caatagaatg tgataaaaga tatactgaaa aacttacaaa agaagcaatt
    21721 aaaataactg gtctaaataa tccaaatagt ccagctcaat taaaaaaatg gttaagtgat
    21781 aaagttggct ttgagattac aagcctaaca aaagaaagta ttccagaaat attaaaacaa
    21841 gttgatgatg aaaatgtagt tagaatttta gaacttagaa aattaatgtc caaaacttct
    21901 ataaagaaat atgaggctat gaaattagct aaaggtaatg acaatagagt aagaggtcta
    21961 ctacagtttt atggggctaa taggactggt agatgggcag gaagattagt acaagtacag
    22021 aatttaccac aaaatcatat agaggattta gacctagcta gaaacctatt aaaagaagga
    22081 gattttgatt taatagagct tttatatgat agtgtaccag atgtcttaag tcaattgata
    22141 aggacagctt ttataccaag tgaaggtcat agatttatag tatcagattt tagtgcaata
    22201 gaagctaggg ttatagcttg gttcgctggt gagaagtgga gattagatgt atttaattct
    22261 cacgggaaaa tatatgaagc tagtgctagt catatgttta aagttccaat agaaagtatt
    22321 aaaaaaggtg acccacttag acaaaaaggt aagatttcag agctagctct tggatatggg
    22381 ggaagcatag gagctttaac atcaatgggg gctataaaaa tgggtcttga tgaagaggag
    22441 cttcaacctc ttgtaacaac atggagaaat gctaatccaa atataactaa attttggtgg
    22501 gatgtagata aggcagctaa aaaagccata aaagatagga ctatagtaaa actccaacat
    22561 gggattaaat ttatttataa tccaggagtt ttatttatag aactaccaag tggtagaaga
    22621 ttatcatatc taagacctaa gatagagcct catactacat ttagtgggga taagataaca
    22681 tatgaaggta tggaacagac aagtaaacaa tggaaaagaa tagatactta tggacctaag
    22741 ttagttgaga atattgttca agctacagct agagattgct taagagaggc tatgtttaat
    22801 gtgacagatg caggttatag cattgtaatg catgtacatg atgagcttgt aattgatgta
    22861 gataaaaaag gaggttcttt agaagaagtt aacagtatta tgggaaaaga aatatcttgg
    22921 gctaaaggtc ttcctcttaa agcagatgga tatgaatgtg attattataa gaaagactag
    22981 gtgattgatt tatggatata aaggccagtg aaattgaaca cataaatgta agacatgatg
    23041 gccaactcat gctggccata ggaaaaaata aattagaaac acattggaaa aataaaagta
    23101 ttttatggtc cgaacttgta aatagattaa gtaaaacatt aagaactcaa gaaacatata
    23161 cagaatatag gaagatgtca aagactgaaa aagatagagt taaagatgtt ggtggatttg
    23221 taggtggaag cttaaaaaat ggaagacgaa aagcagagaa tattgcaaat agaagtatta
    23281 taactttaga tatagattat gcgaataaag atatatggga ggatataaca ttattaaatg
    23341 attatgcttg tcttatgtat tctacgcact cacatactga gaataatccc agatatagac
    23401 ttgtaatacc tttaactaga ccagtactac cagaagaata tcaagcaatt tccaggatga
    23461 tagcagatac tataggaata gatatgtttg atgatactac atatcaacct catagactta
    23521 tgtattttcc aagtacttca attgatggag actatatatt taaatttcaa gatggagagt
    23581 ttttaaaccc aaatgaaata ctagatttat atttagactg gacagatgta agctattggc
    23641 cagaaagttc gagggagagg caaaagttta atacacaatt aaaaaaacaa caagacccta
    23701 ttgaaaaggc tggaattata ggtgcatttt gcaggtccta tagtataaaa gaaactatag
    23761 aaaatttctt aaatgaagta tatattcctg gtattgatga aaccaggtat acatattcag
    23821 aaggtagtac aagtggtgga gtagttattt atgatgacaa gttttcatat agtcatcatg
    23881 gtacagaccc agcaagcgga attttatgca atgcttttga tttagttagg atacataaat
    23941 ttggtgaact tgatgaagat gctaaacccg aaacgcctgt aaatagatta ccttcattta
    24001 ctcgaatgag cgaatttgca agcagtgaca ctaaagtacg aaagactata ggaagagaaa
    24061 accttgataa agctaaggat gattttggtg atatagattt tgaagatgat gaatggttaa
    24121 ctaggttaga ttatgacaat aagggaagtt ataagaaaac aacaaacaat atcttaatgt
    24181 ttatagaaaa tgacccatat ttgaaaggaa aaatagctta taatgaattt tcaaatagag
    24241 ctgtcgtttt aggtaagtta ccttggagaa aagatgataa attaaatgat tggaatgata
    24301 gtgatgattc tgggcttaga catcatatag aaacaattta caatatctca tcaccatcaa
    24361 aagtaaatga tgctctaata attgcttttg aaaataatac ttttcatcct ataaaagatt
    24421 atttaaattc tttaaagtgg gatggcatta agagagtgga tacactttta attgactatt
    24481 taggtgcaga agataaccac tatacaagga ctataataag aaaagtttta gtagcagcag
    24541 tagcaagagt atttaatcca ggaataaagt ttgataatat gatggtttta tctggtcctc
    24601 aaggaatggg aaaaagtact tttattaaaa aacttggtgg agactggtat tctgatagtt
    24661 taactactgt acaaggtaaa gaagcgtatg aacaattaca aggagtatgg ttgctggaaa
    24721 tgggtgaaat gatggctact aaaaaagcag atattgaggc agttaagcat tttctaagca
    24781 aatcagagga tatatatagg gtcgcatatg ggaagagaac atcaaggttt ttacgtcaat
    24841 gtgtagttat aggaacaact aatgataaag aatttttaag agataagacc gggaatagaa
    24901 ggttttggcc aatagataca ggagtaaaga aggttaacaa gagtatattt aatggtcaac
    24961 ttgataatga aagaaatcaa atttgggcag aagcagtaga attatgtaaa gccaatgaac
    25021 agctatatct atcagatgag gagaaaaaag aagctgaaag acaacaaaga actcactcag
    25081 aagaaaatgc taaatctgga attatcgaag agtatctaaa taaacctatt actgagaatt
    25141 ggtatgattt aagtatttca gaaaagagag aatatattca tggttcagat tttggtgatt
    25201 taaaagaagg aacaatatta agagaaaaaa cgtgtgttat ggagatatgg gttgagctat
    25261 ttaatggaga acctaaacaa ctcacgccta tcttatcaag agaaattaat gatatattaa
    25321 aaggattaga tgattggata tcatataatg gaaatttaag gtttgggaaa atatatggta
    25381 ggcagagagc ttatgtacgt aaaagttagt gtcaacagaa ttaaaaacag agtaaatttg
    25441 aaaattttaa aagtgtcaac aatgtcaaca aaaaaaatgg ctttgttgac gcttatgttg
    25501 acacctaaga tgcagtaatt acaatgtttg aggtattaat gtcaacaatg tcaacaaaaa
    25561 ttaagtataa agtagtataa gtaatattag gcatatacgt atatacatat gtatgcctaa
    25621 tatgtatata tacatatata tagaaaatct gttgacattg ttgacactta aattttaagg
    25681 aagtgatatt ttgttagaat caaaaataga aaaaagactt aaaaaagaga ttgagttgtt
    25741 aggtggaaag gctatgaagt ttatttcacc aggtgaggca ggtgtgccag ataggattgt
    25801 cttattacca gaaggacatg ttatatttgt agaacttaaa gcaccaggta aaaaactaag
    25861 gaagcttcaa caatataaaa tgcgagaatt aagagaatta ggatttaaag ttaagtgtgt
    25921 aagcacatta aaagagatag atgattttat caaggaggtt aagggatgga atttaaacca
    25981 catccatacc aagaatacac aattagaaaa actatagata ataataatat aggtttacta
    26041 ttagatatgg gattaggtaa aacagtctgt actctaacag ctataagtga attaatgtat
    26101 gattactttg atatatcaaa agttttagtt atagcacctt taagagttgc aagagatacc
    26161 tggagtagtg aagtaaaaaa atgggaacat ctaaagcatc taaaagtatc taaagttctt
    26221 ggtagtaaat tagatagagt gagagcttta agtacagact cagatattta tataataaat
    26281 agagaaatgg taccctggat agtggatttt tataaaagaa aatggccatt tgatatggtt
    26341 gtgatagatg aacttagttc ttttaaatca aataaagcac aacgatttaa aagtttaaag
    26401 aaagtattgc ctttaactaa aagagtagtt gggcttactg gtacaccaac accaaatagt
    26461 ttaatagact tatgggcaca gatgtactta cttgatagag gtgaaagact aggtaaaact
    26521 attacaggat ataaagaacg atactttgag ccaggacaaa aaaattatca aacaggagct
    26581 atatataatt ggcaacctaa aaatggagca gaaaatgcaa tacataataa aataaaagat
    26641 atttgtataa gcttaaaagc agaagattat ttgaatatgc ctagaaaaat agataataaa
    26701 attgaaatac accttgatag taaaatactt aaatattaca aagaattaga aaaagagaag
    26761 atattagaat tagatagaga tataataaca gcttcatcag cagctgtagc agcaaataaa
    26821 cttttacaat tagctaatgg agcaatttat gataatgata aaaatgtaaa ggaactccat
    26881 agagaaaaat tagaagcttt aaaagaaatt atagatgttt caaatggtaa gcctattata
    26941 gttttttata actataaaca tgattataat cgtttaatga aagagtttaa gagtttgaaa
    27001 ccaagaacaa tagaaaactc aaaagatata tatgattgga ataatggaag aatacaatta
    27061 ttactttgtc atccagcaag tacaggacat ggacttaatt tacaatcagg tggaagtata
    27121 attgtttggt ttggattaac ttggagctta gaactgtatc aacaagctaa tgcaaggctt
    27181 tatagacagg gtcaaaggga aactgttatt atccatcatc taatctgtaa aggtacaata
    27241 gatgaacaag taatggaagc tatagagaat aaggataaag ggcaaagtgc attacttgaa
    27301 gcagtaaaag caaaattaaa agaatatagg gagtgaaggt atgaaagtaa aaagaaaaga
    27361 tggaacttat aagcccaggt aaaatagtaa gatgtgcttg gtgtggtaga agattttata
    27421 agttggataa atctaaagtt gagtattgca gtagaagttg ttcgaagaga gcaagaagga
    27481 gtgatttaag tggataaaga aagtataaaa gaaattgtaa gagagttaag aaaagaagaa
    27541 agagaagata ggaaaaaagg cgttttatat aatactagat tattgatgaa gcattataat
    27601 gattttaaga gacatataga ctcagcggta agtgaagcta aagatgtaga ttacttagag
    27661 gatgatttag gtaagcttga taatgaagag ttgtatattt taagtataaa gaaaagtaaa
    27721 gcaagaacta ttattatgat agcacatata gattcagcaa tgaaaacttt gaaattaaga
    27781 caagagcgtt tacattctta tgaaaagtat agagctttag agctttacta ttgtgatgaa
    27841 aaaacatatg aagaaatagc agagatatta aattgtggag tagttacatc aagacgttgg
    27901 attaatgaaa tggtaaaaga acttggagtg tatttatttg gtatagatgg cttgaaatta
    27961 actgtttgat aaatacatga taaaaagatg atattttaat gatatttaac ccctgttata
    28021 atggtagtat gaaacaagtg tataatatat tcccccttga aaaaggctaa gttaccccaa
    28081 cttagtcttt tttattttgt aaataaggag aatgataaat gagtaattca tgacttagag
    28141 tgatttctag gtcttttttt atacaatttt aatctctcat attgaagggt cgaaaataag
    28201 ggtgttcaaa ttgaatattc tttattaata actaatttag gaggtagtag tatgttgaaa
    28261 attttacaag agaaaaatgt aaggatgata tggtcaaaaa atggagaaga agtttggttt
    28321 aatgcaaatg acgtaggaga ggaactaggc atagtaaata ttcgtgatac attaagaaat
    28381 atagatagag aatataaaaa gaaatttaat gagtctactg tcggagattc ctacactaga
    28441 aactttaaag ataaattgcc taactttggt actacttttg ttacagaaga agctgtgtac
    28501 aatatgtcat ttagaagtaa taaagcagaa gcaaaactat ttacgaagtg ggttacaaaa
    28561 gcacttaaac aaattagaat acatggttac tatattgcca cagaaaaaga tcaagaatgg
    28621 ctggatataa gaacagaagg taaaaggtca gaaaagattt tacagatgaa atacaagagt
    28681 ttttttatta atacaagtac ttgaaagagt tatatctatg aaattaccta ggttgataga
    28741 taaaggtatg aattataaag aggtatataa aaagattaaa gaattaatcg aaatgattta
    28801 aatgactgta ttaatagagg gtcttttttt ttatgtaatg aattaaaaat gaaatatatt
    28861 tgtacattat agacaggaat aaggtaaaag tattatttag acttattttg tggttgtcga
    28921 atgattattg aaggatattg actgattata tagaattata tcattataaa tatttgtgaa
    28981 aggaataatg gtataatgag taattttaaa aaaagatata ttgatgttta tagaatagat
    29041 attataaata aaattaatgg gagtgtctat tcagatgaag aaattttaaa agaggtaata
    29101 aagaccttct ttaaggataa agaatattgt aaagaaaaca aaaatggaat taatattact
    29161 aaagataaaa agttttggat tacagaagta aaatgtagcg aggatgattt aataatcaag
    29221 gtgaaacttg aatatactaa atacaatcag aatacaaata ttataaatgc tcatactaaa
    29281 gaagtagagg gtaataaaaa aatggatcaa ggagatagca ataagcagca cctatttatt
    29341 aaatttatga aggaaaataa tatagcagta gtattatttg aaagagtttt tgtgggggtt
    29401 cccatgattg aactaagtaa aaacttacat ctatattatg aggatatgat tgaaaataat
    29461 aaatttgata ataatatgtc aaaaacatca attaatatta cacaaatagc aagtaaagat
    29521 tttatagaac aaatattaga tttagatggt gtttcaaaaa taactttaaa tgttgataga
    29581 gagaagtttg gaactgatga agataatttg ttttctaatt taaataattc aagacgacat
    29641 aacgatttga tatataagcc cacatttaga actagatatt tacctagtga agttaaaaga
    29701 tgttgcgaaa aatatattaa aggagaggaa tttagaaata aaaaaataaa taggataata
    29761 attgagggag agaaaaataa gagaaaatta aagttggata ctgagggtat aaagttgtgt
    29821 agagaaatag aggtttctct tgatatagat aatcatatag atagtgaaga catttttgag
    29881 aagatgaata atttgataga tgatttaatt gagaataatt cagttttttt taatttaatg
    29941 tatcaagaag ttgctatgag tgaagcatag attgctaagg aggtgttaaa atgtataaaa
    30001 aggtattaaa tatcttactc aattattaca aggctataaa ttctaaagaa gtagtagagt
    30061 tagttttaac accagtatta atatgtatta tttcatacat gttttttaat tcgtcaatta
    30121 ataaagaagt tgtaatttct ttaaacaaag atgtactaac tttgtcagga ctattagtag
    30181 cttttggagt ttgcattatt acattattgt ttactacata taacaaatca atatcagaag
    30241 caaaagatat aaaaacagaa agaaaggtaa atggatttaa tatatcatac tttcaattca
    30301 tacagttaaa agcatattat actgtaataa tggagatttt ggttgttgta atatgtttta
    30361 tcaatacaat aatgcttact cgatattatt ctaatataat tttctatgtt ttaatatttt
    30421 ttacagttca tataatttta tctctaacta cattaataat aagtatgttt catttatcct
    30481 ggaaggatag aggagactag atttgaaaca atcctattaa ataggattgt ttttttattc
    30541 ccaaaacaaa caaataaaga ggtggtgatg tgcaagatgt caaagaaaag gtaaaacaag
    30601 attacttaaa aggaatgaaa caaaaggaaa tatcagcaaa gtatgacatt agtttaaata
    30661 ctttaaagtc atggataaaa agatacaatt ggtctagtga aaaaaagaag ggtgcaccta
    30721 taaataaaag gggtgcaccc ttttctaata aaaattcagt tggttatggt gctccaaaag
    30781 agaataaaaa tgctgaaaag tttggtttct tctcaaaata cttacctgaa gaaactaggg
    30841 aattaataca agaaatatct ataaaagata aatttgatat tctttgggaa cagataacaa
    30901 ttcaatacgc agcaataata agagcacaga agataatgta tgttaaagat aaagaggaaa
    30961 tgattaagga attaaagaaa catgaaatca cagaaaatag tgagaagata gagtatgaat
    31021 ttcaatttgc atgggatagg caagcatctt ttcttaatgc acagagtaga gctatgagtg
    31081 agttaaggag tttaattaag cagtatgatg aaatgattca taaggattgg aatttggcta
    31141 cagaggagca gaaaacaaga gttgagaagt tgaaatgtga ggttgctaac ctaaataaag
    31201 atgatattgg agatgatgag ttgaaaataa gtgtagatta tggtgataga aatgatagtt
    31261 agaataaatt ttaatccaga tttcaaggaa gctaatttta ctaaaaaaag atacagagca
    31321 atgaaaggtt cagcagggag tggaaaatct gttaatgtag cacaagacta tatactaaag
    31381 ttaggagata agaagtatca aggagctaat ctattagtag ttagaaagtc agaagctaca
    31441 cataagtatt caacgtatgc agagcttaca ggagctataa atcgtattta tggtaaacaa
    31501 gctgataagt attggaaaac tactttaaat cctttagaaa ttaagagtaa agttactggt
    31561 aactctataa ttttcagagg agttaatgat gcaaaacaaa gagaaaaatt aaaatcaatt
    31621 aacttctcga aaggaaaatt aacatgggtt tggtgtgaag aagctacaga acttatggaa
    31681 agtgacatag atatactaga tgaccgttta agaggtattt taactaatcc taacttgtat
    31741 tatcaaatga catttacatt taatccagtc tcagctactc attggataaa aagaaagtat
    31801 tttgactata aaaatgatga tatatttact catcatagta cttatctaca aaatagattc
    31861 atagatgaag cttactacag aagaatgcaa atgagaaaag agcaagaccc agaagggtac
    31921 aaagtatatg gtcttggaga atggggagaa actggtggag caatacttaa aaattatgtt
    31981 atacatgaat ttcccacaga atttgaatac tttgacaata tgagactgtc acaagacttt
    32041 ggctttaatc atgcaaatgc aatacttaga attggcttta aggatggaga gttatatata
    32101 tgtaatgaaa tatatgtaca tgaaatggat acctcagaaa tcataaaaat tgcaaatagt
    32161 agaggtttag aaaagactct atttatgtac tgtgatagtg ctgaaccaga tagaattaag
    32221 atgtggaaga gtgcaggata taaagctaaa ggagttaaaa aaggaccagg aagtgttaaa
    32281 gctcaaatag attatttgaa acaattaaga atacatgtac atcctagttg cactaatacc
    32341 ataaaagaaa tacaacaatg gaaatggaaa caagacgaaa gaactggatt atatcttgat
    32401 gaaccagttg agtttatgga tgatgcaatg gctgctctta gatattctat agataataag
    32461 cttaaaaata atggaataag cttcttaaag taaaggaggt gttaaatatt tatataagtg
    32521 aaacagattt aataaaagtt cagttaaaaa aagagagcac ctttaaccta gtaaaagtca
    32581 tagaacacta catcttaaag cataggccag aaaaatataa acaaggagaa gaatactatt
    32641 atggtaatac tgatgtaaac aataagagaa gatattatct cttagatgga gctaaggttg
    32701 atgattttac taaagttaat aataaagcaa ttaacaacta ccataagctt ttagttgacc
    32761 aaaaggtagg ctatagtgtc ggaaatccca tagtatttaa tgcagatgat gataatctca
    32821 ctaagctttt aaatgactta ctaggagaag agtttgacga tacaataaca gaactatatc
    32881 tcaatgctag taataaaggg gttgaatggt tacatccata tattaataga aaaggtgagt
    32941 ttaaatatgt aataattcca gctgaagaag caattcctat ttgggatagt aaaagacaga
    33001 gggaattagt tgcatttatt aggttttatt atattgaaga tatagatgga aataaaataa
    33061 aaagagttga gtactacaca gaaaatgacg taacttactt tattgaaaga ggtaatagtt
    33121 ttattcaaga atttttatat gatgaatatg gaaaaatgac tgatatacaa gaaggtcatt
    33181 ttagaataaa taacaaagaa cagggatggg gtaaagttcc atttatacct tttaaaaata
    33241 atgaaaagtg tgtctcagat ttaactttct ataagtcttt aattgatata tatgataata
    33301 acatttctac acttgcagac aatttagatg aaatgcaaga ggtcatttat gtattaaaag
    33361 agtatccagg aacaagtcta caagagttta tagataatat aagatactat aaatcaatta
    33421 aagtagatgg tggaggtgga gttgataaac tagagataaa tataccagtt gaagccaaaa
    33481 aggagcttct tgatagatta gaaaagaata taattatttt tggtcaagga gttaatcccg
    33541 aatctcaaaa cacaggtgac aaatcgggtg tagctcttaa atttttatat tcactacttg
    33601 atttaaaatg ttccaagact gaaaagaagt ttaaaaaagc aattagagag cttttatggt
    33661 ttgtgtgtga gtatttaaag ataagtggta gtaagagcta tgattataag tcagttcaaa
    33721 taacatttaa tcattctatg ataataaatg agtctgaaaa aatagatatg gcttctaaat
    33781 caattggaat tatatcagat gaaactattg ttagtaatca tccttgggtt gaagatgtta
    33841 atgacgaact tgaaagactt aaaaaacagg aagaaaccca aaaagaatat gatgatttaa
    33901 ttcctactaa taatcaagag ggtgttatag atgaaacata aagattattg gaggaaaaga
    33961 tttgaacaat tagaagaagt tcaaaacaat aaaagtgtaa aatattatct tgaattggaa
    34021 aagaaataca aattagctat gaatagtata gaaaaagata tattatcatg gtataacaga
    34081 tttgctgaaa atgaaggaat atctttatta gaagctaaga aactgttaaa tacaagagaa
    34141 ttagaagaat ttaagtggaa tgtagaagag tatattaaat atggcaaaga aaatgctata
    34201 aatcaaaagt ggatgaagga gttagaaaat gctagtgcaa gacttcatat aacaaggctt
    34261 gaagctttaa agttacaaat acagcaacac gtagaagttt tatatggaaa tgaacttgat
    34321 ggtattgata aactaatgag agatatttat acaagtggat actatcatac agcttttaat
    34381 gttcaacaag gagtaaacgt tggttggagt ttaatgagtc ttgatactaa tagaataaat
    34441 aaaattatct ctaaaccatg ggcaacagat ggattaaact ttagtgaaag aatttggggt
    34501 aagcatagac ctactttaat aaatgaacta catactaagc taactcaatc aattattaga
    34561 ggtgaaaatc caaaaaatct agtaaatgac tttgctaaga gatttaatgt atctaaatca
    34621 caagctaaga atttgataat gactgaatca gctttctttg catcagcaag tagaaaagat
    34681 tgttttagtg atttagatgt agagaaatat gagattattg ctacattaga tttaagaact
    34741 tcaaatatat gcagagagtt agatggaaaa atatttgata tgaaagatta tcaagttgga
    34801 ataacagctc caccatttca ttgtcgttgt aggacaacaa cagctccttg gttcgaggat
    34861 gaagaaggct atagagcagc aagaggagaa gatggaaaaa catattatgt accatctagt
    34921 atgaagtata atgagtggta tgagaaacat atagagggta gacttggaaa agaaaaagct
    34981 gacaccctta gaaaaatgca actaaatgag agtaaagata gaaaacaatt tgaagaatat
    35041 aaaaaggtat taggaaatga aataccttct aaatttgatg aatatcaaca tatgaagtat
    35101 aatgatacta taaggtatga ggaagctaaa aagctttata aggatgtaaa ttggcaagtt
    35161 aagaatcaga gaaatttaac tagtggaagt gttcattcag tgccatttga atcaaaacca
    35221 aatagtgtat ttgataatta caaagatggt aaattaattc aaagaagata ctatggtaat
    35281 acaggaaaac ctagattaga tttagactta actgaccata gaaacccaaa gcaacataaa
    35341 attgtacctc ataaacatga ttggttagct gatgaaaata accatgataa ggtaaaaaga
    35401 gagaaggata tggagcttac aaaagctcat aaaatagcta acaaagatat tttgaatgga
    35461 gaataaaatg gataataaat ttaagaattt acaacattta attgattcta ttgatttagg
    35521 attagatata gaatttgact tatataataa accatataat atatctattg gagatgatga
    35581 tacaaggttt attactcttt gccctaatgg agatacaaag tattataaaa atggtaagga
    35641 tatggttgat aattacaaca ttgatggtaa attactaaag gatttatgga aagatataca
    35701 aatagtaaat atgtaaaagc acttgctaaa taataaatta gtaggtgctt ttattttgta
    35761 aaaaatgaaa ggagaaattt aaaatggatt ggttaaaaga attgctagaa ggaataaaag
    35821 tagaggataa caaaattgat gtagcttctc ttcaaaaatc tatagaaaag aaaataaagg
    35881 agactacagt tactcaagaa gattatgtaa atcttgaaac acaacttaat acagctaatg
    35941 aaactattaa aaagtttgaa ggaggtatga caaaagaaga tgtagagaat ctaaaaacaa
    36001 cttatgaaac tgataagaaa actttggaag aaacctacaa aaaagaaatt gaagaaaaag
    36061 attttaatta ctggttaggt gatgctttta aatctgttaa atgtagagat gaaatagcgt
    36121 taaaagctca tttagacatg gaagccctaa gaaatagtaa agatagacaa aaagcttttg
    36181 aagagcaaat aaaccctttg aaacaggata aagattattt gtttaatgca acactagaag
    36241 gtgaagaacc taaaatagat actataacac cagggcaaga gcctaagata aatgattttg
    36301 gttttaattt tactggggta agacctcatg aaaataataa taaataggag gaaataaaat
    36361 ggcagcacta aattatgcaa aagaatattc aaatgtttta gcacaagcat atccttatac
    36421 tttaaacttc ggggatttgt atgcaacaga aaataatgga agatatagat ggacgggttc
    36481 taaaacaata gaaataccaa ctatatctac aactggaagg gtagattcaa acagagatac
    36541 aatagcagta gctcaaagaa actatgataa tgcttgggaa cctaaggtat taactaatca
    36601 aaggaaatgg tcaacattgg ttcatccagc agatataaac caaactaatt atgtggcttc
    36661 aataggtaat ataacaaaag tatataatga ggaacaaaag tttccagaga tggatgctta
    36721 ctgtatatct aaaatatatg ctgattggac cgcattaggt aacacagcag atacaactgt
    36781 tcttacaaca gcaaacgtat tagaagtatt tgataagtta atggaaaaaa tgacagaagc
    36841 tagagtacct gaaaatggaa gaatattgta tgttactcca gtagtaaata cacttatcaa
    36901 aaatgcaaaa gagatacaaa gaacagtaaa cataaaagat ggtggaactt ctttaaatag
    36961 acaaaccaca gatattgaca cagttaaaat aattaaagta ccatctaatc taatgaaaac
    37021 tgcatatgat tttacaactg gatggaaagt aggagcagga gctaaacaaa tctttatgtc
    37081 cttagttcac ccaagtgcaa taattacacc tgtttcttat cagttctcta agttagacga
    37141 accaacagca gttacagagg gaaaatactt ctactttgaa gaaagttttg aggatgtatt
    37201 tatattaaat aaaaaagctg atgcaataca atttgttgtt gaagcataga aaaaggagtg
    37261 atatatagtg ccacaagtaa aaaaactaaa tagaatacta accatagaag aatgtaaaat
    37321 agatgatttc ttagagatgg gatatgattt gatagatgaa actggtaagg cagtaaagta
    37381 tggcaagtca ttaagtgtaa aagatttaat agctgaaaat aatattttaa ggtcaaaagt
    37441 tgagtcttta gaagaagaaa ataagcagct taaagagaaa aataaactta ctaaaaagta
    37501 ggtgaaaatt atggaaaata atataattga tgaaatagaa aaaagacttg aaagttttgg
    37561 atatatatta aaagatggag ataagtggtt aataggtttt gtaagagaaa aaatagaaaa
    37621 tattattaaa ctagattgta atataaaaac tatgccaatt gaattgaaag aaattgaagt
    37681 tgatatgata gttggagagt tcttatttac caagaaaaat atgggtcaat tagatataga
    37741 aagcattaac tttgaagctg tagaaaagtc tatatcagaa ggtgatacaa aggtagattt
    37801 tgctatagga agtggttctc aaacaccaga acaacgcttt gatagcttaa tagcttatct
    37861 tactacttat ggtaagaata agatattaac ctttaggtgc ttaagatggt aagtaaaact
    37921 agaaaagcaa tagaaatgtt atatagagat aaatgtacta tagttgagta tcagccaatt
    37981 aaagaccctg taacaaaacg aactaacaat aaagaagtga ttgtattaga aaatcaacca
    38041 tgtaaacttt catataaaaa tatagtttct gctacagaag ggaaattagc taagctagag
    38101 caaactatta aactctttat atctccagat atagaaatta aagcaggttc aaaacttatt
    38161 ataaatgata aagagtatgt aagaagtgga gaatcagcta tatatccaaa tcatcaagaa
    38221 ataatacttg agttatttaa ggataaagca taatggctag atggggcagt gttgatttta
    38281 gagagtttaa aagagtttgt aaaaagatgg agaaatttac aaagattgat ttagataagt
    38341 tttgcaagga tgcagcaaga gaattagcag caagactctt aggaaaagta attagaagga
    38401 caccagttga tacaggattt ttgagagagg gttggagtgg agtagcttat gctaggtcgc
    38461 ttcctgttta caaacaaggt aataattata ttatagaagt tgttaatcca actgaatatg
    38521 ccttaacaca atggggcata taaaacccag caaaatcggt aaacgctaag tgtaatagtg
    38581 ttacattata aagtaggtag aatatggaca tagaataact cttgatttat aatgtataaa
    38641 agaggtgatt ttatggcaaa atttttagat attacaggaa aaaaatttgg aaggctaaga
    38701 gtcattaaat tttctaaaga gataaaaagc ggtaaaagaa ataggaaata ttggttatgt
    38761 aagtgtgatt gtggaaattt caaagaaata agaactgact ctttaactag tggcttagtg
    38821 caatcttgtg gatgtttaaa aaaagaacaa gataaattaa atttaacaga caaataccaa
    38881 tttaaaaaga aatataaggt tcaaaataaa agactttaca gtatatggaa gggtataata
    38941 tctagatgta cagataaaaa taataaaaga tacaatagat atggtgaaag gaatataatt
    39001 gtgtgtgatg aatggttttg ctatgataat tttgcaaatt gggcattgag taatggatat
    39061 tcagagaaac ttacaattga tagaataaat aatgaaggaa attatgaatc aagcaattgt
    39121 agatgggtgg atataaagac acaatgtaga aataggtcaa caaatatatt agtaaaacat
    39181 gaagaaaaag aaattacatt gatagagctt tctgaaaaaa caggtatttc atactcttgc
    39241 ttaagaagta gatattctaa gggattagta ggaaataagc ttatagaaaa agttaagatt
    39301 attgaagaaa gcagagctaa gttatctatt gaggatgtaa aagaaattag aaaaaagtat
    39361 tctgatggat atacaataaa acaactaagt gaaatatatc ctgtaacata ttcatctata
    39421 tcaaatatag tccatagaag aacatggaaa aatatttaat taattatata tgccaatacc
    39481 gaggaaaacc tatagattgc ggataggcta taggtttccg tagagcgtag agagtgaata
    39541 aatataatct ctccaagagt gctgggcaac taaataaagt ttattttgtt gatgatgtac
    39601 gccgaactta taggaaacta taagagctag aggataaaaa gcctttagga taacaaaatg
    39661 catatgttga atttggtcat agaactaaaa atggcaaggg ttgggttaaa ggacaacatt
    39721 tcttaactat ttcagaaatg gaactacaaa gtcaagttga taagataata gagaaaaaac
    39781 tattaatatt gcttaaagga gtgtttgatg cttaataata ttatagatgg aatatcagta
    39841 aagttagata aatcgtttgg agaaaaatat acaatttata gcgaagatgt ggagcaaggt
    39901 attaatgaac cttgtttttt tattgttcct ttaaatccaa gcaagacacc atatccaagc
    39961 gggagagaat taaagaaaaa ttcttttgat gtacattatt tccctcgttc agaagataag
    40021 aattttgaaa taaatgagat agctgagatg ctactggagg aattagagta tatagaaatt
    40081 gatggagatt tagtaagagg tacaaatatg aattttgaaa ttatagacaa tgttcttcac
    40141 ttctttgttg attataacta ttttactata aaaaataatg ataccaataa gatggataca
    40201 gtagagttat tcggtggttt gaagagaggt gataattttg agtaaagcat taagcaaaga
    40261 agataactac aagtttacta aggagcagat agttaactct aagaagtatg taaatagaaa
    40321 agatttatta aatgcaattt taaaagaaaa tgagttatat tccttctcag aggtagagga
    40381 tagaataaat aaatttatga aaggagtgag ttagatggct ttaggtggag gaacattcgt
    40441 aacgcaaaat aaaatacttc ctggtgcata tataaatttt atctcagcta agagggcaac
    40501 cagttcatta tcagatagag gtattgttgc aatgccttta gagttagatt ggggcataga
    40561 tgaagaagca ttccaagtaa ccagtgatga ttttgagaag tattcagtga agtattttgg
    40621 atatgattat actcatgaga agctgaaagg tttgagagat ttattcaaaa atataaggtt
    40681 gggatatttt tataaattaa ataaaggcgt taaagccagt tgtactatag ccacagcaaa
    40741 atatagtggt ataagaggaa atgacttaaa agtaacagtt acaacaaata tagatgataa
    40801 tgctaagttt gatgttgtaa tacttttaga taataagaag gtagatactc aaatagcaaa
    40861 ggttattaca gacttacaag acaatgacta tatcacttgg aagaaggatg caacactaga
    40921 agcaagtgca ggacttgtat ttactggtgg aaccaatggc gaagctgtga caggagcaga
    40981 gtaccaagct ttcttggata aaatagaaag ttattcattt aatgcactag ggtgtttggc
    41041 tacaacaaca gaaattaaaa gtttatttgt agaatttaca aagagaatga gagataaggt
    41101 aggagctaag tttcaaacag tactatataa gaaaagtgat gcagattatg aaggtgtagt
    41161 gtctatagaa aataagatta aagataaaga tttagttgaa tctagcttga tttattgggc
    41221 tactggagct atagcaggat gcgatataaa taaatctaat actaataaaa agtatgatgg
    41281 tgagtttgat gttgatgtta attatacaca aatacaactt gaagaagctt taaaaactgg
    41341 taaatttata ttccacaagg tgggagatga agttcatgtg ttagaggata taaatacttt
    41401 tgtatcattt acagatgata aaaatgacga tttttcaagt aaccaaagtg ttagagtact
    41461 tgaccaaatt gctaatgata ttgcaacttt atttaatgaa aagtatttag gtaaagttcc
    41521 gaatgataag gcaggaagaa taagtttctg gaatgatgtt gttaaacacc ataaagaatt
    41581 agagaatata agggcaatag aagattttaa aactgatgat gttagtgtag agcttggaaa
    41641 tgataagaaa actgtcatag tatctgatgc tattaaggta ataaatgcta tgagtaagct
    41701 ttatatgact gtttcagtta attagagagg agagtgataa tatggctcaa acaataaatg
    41761 ctaaagatac agttagtgca aagaaagctg aatgttttat aactatagaa ggtaaaagat
    41821 ataattttat gcaagctata gatttagagg ctaaaatgga aaaaaataaa agtgaagttc
    41881 caattctagg aagaacaaca aagggaaata aaacaactgg gagtacaaat actggaagtg
    41941 caacatttca ttataatact tctattttta gagaattact ttacagatat aaagaaactg
    42001 gtgaggatat ttattttgac atacaagtta caaatgaaga ccctacatct gctgtaggaa
    42061 gacagacagt agtacttaaa gattgtaata tggacagtgg aataattact aaatttgatg
    42121 ctgatggtga gtatttagat gaagatatgg atttcacttt tgaggattgg gaattagttg
    42181 aatcctttgc attacttaat ggaatgttat aaaattgaat tatacttgaa aaatattatg
    42241 atgtaagtat aaattatttg gaggtatttc atggaatttg aaagtggaat taaattaggc
    42301 aaacttactt taattaaata tttagggaga aataaacata gtaaaaaagt ctggctatgt
    42361 caatgtgagt gtgggaataa agttgagaga gtagaaaata atttaaagca atcaattaaa
    42421 aatgagaagc ctccacattg tggttgttct ccaaactgga aaggacaaaa taagagattt
    42481 aaagatataa ctgggaaaac ttttgggaag cttacagtat tgaaaatgtc tgggaaagat
    42541 aaatatagtc acaatttatg gttatgtcaa tgtgaatgtg gaaatagaac aataactagt
    42601 acaagtgcat tagaacaagg aaaagcacaa agttgtggct gtataagaaa ggaaatatta
    42661 ctagagcgct ctacaacgca taataaatct aatgatagct tatatagagt ttatcaccgt
    42721 atgattaaaa ggtgcacaaa taagagtaat aaagattata attattatgg aggtagaggc
    42781 atagaagtat gcaatgaatg gctagaggat tttataaatt tttataactg gtctatagaa
    42841 aatggatata gaaaaggttt aacaatagat agaattaatg tgaatggaaa ttacgaacca
    42901 tcaaattgta gatgggttac ttgggaagtt caacgaaata ataaaagaaa tagtattaga
    42961 gttaattata aaggtgaaat gattacttta aaacaatgtg cagaaaattt gaatgttaag
    43021 tatctatttc tttataatca attagttact aaaaaaattc cattggaaga agtaataaaa
    43081 aatataggta tggagtaaaa tacacattta taagttataa atgtgtattt tttatatgaa
    43141 aaattaaaat aaaaggagat tagaataata tgagtaattt aagtgctttt ttaagtcaaa
    43201 atgcaataaa ggttgataat gtaaaatatg tagcgagtaa cagattttta gataaagaag
    43261 ggaaaccagt tgaatgggaa ttaaaagttt tatcatctga agaagacgaa gcactaagaa
    43321 gaaagtgtac taaaagagta aaagtgattg gtaacaatgg taagcatact ggacaatata
    43381 caagtgaaat tgactacaat agttatgtag ctgaattatg tgtagcatct acagtatttc
    43441 cagatttaaa ggatgccgaa ctccaaaata gttatggagt aatgggagaa gctcagttat
    43501 taaagacaat gcttacagca ggtgagtatg tcaattatac agtaaaagtg aatgaagtca
    43561 atggatttga tacatctttt gaggataaag tagaagaagc aaaaaactaa tcagaggtgg
    43621 cgattttgat gctagcatca ctcattattg tattcaaaaa ttaaagtgga agccaagtga
    43681 atatatgaat ttagaagtta atgagagagc gttagcagcc gcctcaatac ttataaagat
    43741 agaagatgaa gaggaagcaa tgaaagaagc tgaaagagag agaaagaggg gacgaagaag
    43801 atagcaaaat aaaaaaataa atatagaata ggtaaaatat gtaataatta tatgttataa
    43861 tatttttagc aagaagatgt aatctacaat ttatagagtg gagttcatac tgggataaaa
    43921 cctacttcct aatgaaagga ggtgggaagt atgaataact ttttacttaa tgtaatagct
    43981 ggcgttattg ctagtttaat attttgctta atttgtaaag tatttctaaa agtaaaaagc
    44041 cactcaactc gtggcaagag taaaagtggc tgggaatttg attttaaaat caagttccac
    44101 aaatttaaat aattactaat tgtttaaatt atgaacttca ctctactgtc aaatagattg
    44161 tag
//
LOCUS       DAMKPG010000025        43940 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMKPG010000025 DAMKPG010000000
VERSION     DAMKPG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.027"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(51..506)
                     /locus_tag="PC621_001447"
     CDS             complement(51..506)
                     /locus_tag="PC621_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108609.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(734..1153)
                     /locus_tag="PC621_001448"
     CDS             complement(734..1153)
                     /locus_tag="PC621_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108610.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            1452..1661
                     /locus_tag="PC621_001449"
     CDS             1452..1661
                     /locus_tag="PC621_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108611.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1693..1902
                     /locus_tag="PC621_001450"
     CDS             1693..1902
                     /locus_tag="PC621_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108612.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(2119..2517)
                     /locus_tag="PC621_001451"
     CDS             complement(2119..2517)
                     /locus_tag="PC621_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108613.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(2938..5430)
                     /locus_tag="PC621_001452"
     CDS             complement(2938..5430)
                     /locus_tag="PC621_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4108614.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            complement(5444..5986)
                     /locus_tag="PC621_001453"
     CDS             complement(5444..5986)
                     /locus_tag="PC621_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4108615.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            6229..7743
                     /gene="glpK"
                     /locus_tag="PC621_001454"
     CDS             6229..7743
                     /gene="glpK"
                     /locus_tag="PC621_001454"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF4108616.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(7857..8486)
                     /locus_tag="PC621_001455"
     CDS             complement(7857..8486)
                     /locus_tag="PC621_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4108617.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            9075..9866
                     /locus_tag="PC621_001456"
     CDS             9075..9866
                     /locus_tag="PC621_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF4108618.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            9895..11115
                     /locus_tag="PC621_001457"
     CDS             9895..11115
                     /locus_tag="PC621_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF4108619.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            11135..11830
                     /gene="pxpB"
                     /locus_tag="PC621_001458"
     CDS             11135..11830
                     /gene="pxpB"
                     /locus_tag="PC621_001458"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF4108620.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            11860..12846
                     /locus_tag="PC621_001459"
     CDS             11860..12846
                     /locus_tag="PC621_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF4108621.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(13065..13631)
                     /locus_tag="PC621_001460"
     CDS             complement(13065..13631)
                     /locus_tag="PC621_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108622.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            13983..15050
                     /locus_tag="PC621_001461"
     CDS             13983..15050
                     /locus_tag="PC621_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF4108623.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            15069..16229
                     /locus_tag="PC621_001462"
     CDS             15069..16229
                     /locus_tag="PC621_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF4108624.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            16242..18338
                     /locus_tag="PC621_001463"
     CDS             16242..18338
                     /locus_tag="PC621_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF4108625.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(18497..18841)
                     /locus_tag="PC621_001464"
     CDS             complement(18497..18841)
                     /locus_tag="PC621_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF4108626.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            19133..20023
                     /locus_tag="PC621_001465"
     CDS             19133..20023
                     /locus_tag="PC621_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF4108627.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            20241..21527
                     /locus_tag="PC621_001466"
     CDS             20241..21527
                     /locus_tag="PC621_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF4108628.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(21616..22059)
                     /locus_tag="PC621_001467"
     CDS             complement(21616..22059)
                     /locus_tag="PC621_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF4108629.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(22083..22877)
                     /locus_tag="PC621_001468"
     CDS             complement(22083..22877)
                     /locus_tag="PC621_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF4108630.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(23443..23919)
                     /locus_tag="PC621_001469"
     CDS             complement(23443..23919)
                     /locus_tag="PC621_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF4108631.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(24020..25189)
                     /locus_tag="PC621_001470"
     CDS             complement(24020..25189)
                     /locus_tag="PC621_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108632.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            25352..26215
                     /locus_tag="PC621_001471"
     CDS             25352..26215
                     /locus_tag="PC621_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF4108633.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            26286..26879
                     /locus_tag="PC621_001472"
     CDS             26286..26879
                     /locus_tag="PC621_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF4108634.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(27130..27765)
                     /locus_tag="PC621_001473"
     CDS             complement(27130..27765)
                     /locus_tag="PC621_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF4108635.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(27845..28576)
                     /locus_tag="PC621_001474"
     CDS             complement(27845..28576)
                     /locus_tag="PC621_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF4108636.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            28907..31096
                     /locus_tag="PC621_001475"
     CDS             28907..31096
                     /locus_tag="PC621_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF4108637.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
     gene            31507..33435
                     /locus_tag="PC621_001476"
     CDS             31507..33435
                     /locus_tag="PC621_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF4108638.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            33422..33769
                     /locus_tag="PC621_001477"
     CDS             33422..33769
                     /locus_tag="PC621_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF4108639.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(34011..35930)
                     /locus_tag="PC621_001478"
     CDS             complement(34011..35930)
                     /locus_tag="PC621_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF4108640.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(35956..36762)
                     /locus_tag="PC621_001479"
     CDS             complement(35956..36762)
                     /locus_tag="PC621_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF4108641.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(36821..38269)
                     /gene="ddlR"
                     /locus_tag="PC621_001480"
     CDS             complement(36821..38269)
                     /gene="ddlR"
                     /locus_tag="PC621_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF4108642.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(38293..39192)
                     /locus_tag="PC621_001481"
     CDS             complement(38293..39192)
                     /locus_tag="PC621_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF4108643.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            39450..40706
                     /locus_tag="PC621_001482"
     CDS             39450..40706
                     /locus_tag="PC621_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF4108644.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            40776..41555
                     /locus_tag="PC621_001483"
     CDS             40776..41555
                     /locus_tag="PC621_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF4108645.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(41659..42243)
                     /locus_tag="PC621_001484"
     CDS             complement(41659..42243)
                     /locus_tag="PC621_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF4108646.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(42443..43873)
                     /locus_tag="PC621_001485"
     CDS             complement(42443..43873)
                     /locus_tag="PC621_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108647.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT        16011 a         5440 c         6853 g        15636 t
ORIGIN      
        1 aatttgatta gaaaaaatca attttaagac aactcctttt ttttattaaa ttattgtcta
       61 ttaaccaaaa tagctatttt agcatctgga ttataactta tctgaaccat ttgatttttc
      121 ttaacatgtt caaggtcttc accaccataa gctatttgta acttaactgg taacttacct
      181 tgttttataa tagcaacgta ctctttttta ccttttttct ctaaactaat caaattgcca
      241 acataaggtt taaagttctg atacttttta ctagaatttc ttatgtagaa gaaagcacca
      301 acagcaataa ctaaatttat gccaagtgta acccaagaat tgattttaag catagctcca
      361 gcgattatta tcacgaacat taaaacgata ggtaatatag ctttcttaag aagcaattta
      421 cccattattt cattagcctt tttctcaggg ccactcatag tttttgatct agcaaatgat
      481 tgcgcgaatt tgtctcttaa gcccatttta tcctcctaat tttaataaat atttagttat
      541 aataacgaga tattacttga aactaaaaat ttactacatt tatattatgt ttgacttttg
      601 tataaataat tacattcaag taaagcaaaa tatactaatt attttatcat aaaattataa
      661 aaaagaaaat aaatgaaata aaaatattag aacaaagaaa tgatgtaaaa tcgtatcaaa
      721 agcaacataa aaattattta tctattttct catctttatt tttgttatac tcaatttttc
      781 ctaaatcctt ctctttttca tattcatgaa gttttaattc aatcatacct tctattttgg
      841 ctttatcata atcatttaac tttctaaagt tgtttaaaag ctttatttca ttagagttta
      901 tgctattaag tggatagttt gaggaggaat cgcaaattaa atctgattta tgtgatagat
      961 tatctccatt taatagccag tctactgaaa cattaaatat ctcagctata gattttaata
     1021 tttcataatt tggttttcta atgtttctct caaatttact taagttgtca cagcctaaca
     1081 tttcttctag ttcatattgt ttaaggtttt ttgcttttct caaataaaca attctttctc
     1141 ctaaagtatc cataaacact ctccattcaa ttaatgtcaa aaagactttt taagatgtaa
     1201 atagtttcaa attaaaggtc aaaatgacat aaaaaccatt gacttaaggt caaaatgact
     1261 ttataattaa cttaatgata cgaatttaca tcctaatttt agcacaaagt aatcaaaaaa
     1321 tcttatttag tattaaataa atttatatac ttaatatgtg tacatattaa aaatatatac
     1381 taaatagagg gggtgcgtaa gctaaagtaa tataaaagta aatataaatc acttagaaag
     1441 gaagttgata aatggatgct cgaaaaaaat ggataccttt tttgggagtg caagtcaagc
     1501 aaagacttat tgaattaaat atgactcaaa gggaattagc gaagaaaata ggtgttaatg
     1561 aaaactattt gtcagctatt ttaaatggaa gaagaacagg taaaaaatat aaatcatcaa
     1621 tttatcaatt acttaatata gaatattcag aagatgatta ataaatagta tataaagtag
     1681 gtgaatattc ttgtgtgcaa attggattca gatggggtta tagagtgttg tagagcaatt
     1741 gatgatttta ttacagcact tagtaatata aaaagcttaa atatggaaag attaaatact
     1801 ttaactaaat attctagtac atgttcaatc cttcttaaag aggggaatta tgaaggatgt
     1861 acaattgtgt atagaaagat gttggaagaa ttaaaaacat gagtaatgca tttcttagga
     1921 atataaatta tacatagaaa tgtattatat ttttcaaagt acttaaacta aaatatggat
     1981 aagataatct aaatattata aatgtgcttg aaattagact atacttgttt ttaaataatc
     2041 caatatccat attttagtaa tatactacaa aaaaagaagg ttaatagatg atgtaaaatc
     2101 gtatcaaatt atgtatgttt aaaccatttt atcttcatta ttattagagg aatgcttttt
     2161 taagtcttta tattcagata tcttaagttc aagtattcct tctattttta ttttatcacg
     2221 ttcgtttagt tgtctgtata gatttaatat catcatttca tcattagtaa catgtaagta
     2281 atcttcttta tcttctttta cactactatt gacatttacc ttctctttac catagagaag
     2341 ccagtcagtc gtaacattaa aataatcagc tattgacatt agtatatcac aattaggttt
     2401 tctatctcct gtttcatact tgcctaagtt ttcaaatttt aaaatatcca taagtttgcg
     2461 ctgagtaagt tttttggagt ttctcaaata agcaattctt tttcctaaag tatccacaaa
     2521 atacactcct ttctttttat gagtaatgtc taaatgacat ttgaaattaa aaatatataa
     2581 atttataata taaaactact aaattaaagt ctaaatgaca ttttgcttaa attaatatgc
     2641 tcataatatg attttaacat attatagttg aaaatatatg gtttattttg atttgtatat
     2701 ataacaatag atttaattgt tataaaaatg taaaggggtg tatgaataga ttgtataaat
     2761 ttatttcgat aaactaagat tgctttttga ttgtctgtaa aagagaaaaa gattaagata
     2821 aaaatagtat tatattgtaa tttatattaa tcaattacaa agattttatg aatttattct
     2881 ttagggtaaa atatttaaga ataagataaa tttacaatat aatactataa cactctttta
     2941 tctagtttta ttttctttat agaacaataa tattataaat gctagtagat ttacacagaa
     3001 tactgttata tacatctgtt tgaatcctga gtttagagta gattgtagtg tattttctat
     3061 ttttgttcta ttattttcta tggaatttat ataatccaat tttgatttat caaaagcctt
     3121 tggtatttca gtttttacct cttgtgtctt tgctaaaact gcttggagtg attttttttg
     3181 ctgttgcatt gtatcaaggc ttttttccat gtctttcttt tggtttatag aattatttaa
     3241 cttgctttgt agctctttct tttgagattc cattgatctt agttttttac ttaattcgtt
     3301 cttttgagtt ttattttttt gtaactttgt atttaagtca tctcttgtct tttttagttg
     3361 ctgtatttga gcatttaaac tttctttttg ttcatttaca tttggtttat tctcgctgga
     3421 attgttattc ttattttctg tagaatcatt cttagtgtcg tctgattcag aattactggt
     3481 attactctgg ttttgttcat ttgagttatt ttcgttatca gtaggagtct tttctgtagt
     3541 aggtatttta ttataagcag ttgtaagttc cttgatggcc tcatcttgtt tttcaagacc
     3601 ttgttccata ccttttatac ctttgtcaac accagcaata ccttttttta caccatctat
     3661 tccttgatta ataccagctc tagcttttac catattgtca ataccagttt gaactccttt
     3721 aataccacta tttagtttat tttcaccatc ttcaataccg tttattccac tttgtgttcc
     3781 atctattcct ttttgtacat ttttctgtat tttagtaatt atagatggaa ctttttcatc
     3841 aaacattgtt gttgatattt cttttgttat attggtaatg ttggttgtat ctgctgactg
     3901 aactttatta agtaaggatt ttggaagttt gccacctgtc atgtccatat ttatatttga
     3961 agtatcattc atatttggga tagagacatt ttttaaattt tttgccattt cagggtctga
     4021 ttttaagtca tctatcatgt tttcaagttc ttcaagttga actattttag gagttgaggg
     4081 ctttccaact aagtccataa tattatcttg cacagataat cctgcatgtg ctataaatcc
     4141 aatcattata gctggagaaa ttgaagtacc aattgagcgt atcaaggata atgttgcaag
     4201 tgctgaatta gattcttcta atcttgtatt tgataacatc atatagttta gaggagttcc
     4261 catagtaaag cccattccta atcccataaa tattaaactt acacatacac taaatacagt
     4321 attggttttt agagcaatta aaactaggta aagagagcct atcatagata cagaaaatcc
     4381 tagtaatagt attttctttg ccccaaactt gtcaataagt cttccgctta aaggagcagc
     4441 aatacctgca aataatccca atatagctac aaaatatcca ccagaaccag catttatctt
     4501 taatgcattt tcagcatact gtggaacgaa taccataccc atcattccaa taccaacaat
     4561 taaagataaa ataagtacta tcaatattct cggatttaaa aaatattcaa agcttaaaat
     4621 agggtcttta gctctatttt caataaatat gaaaataggt ataagtacaa taaatgtcaa
     4681 taagtatgga taaactgata tatcttgtat agaattttta aaattaaaaa agtctatatt
     4741 cataagacca tataatagtg atacgattat acatatcaac atcaatgtac caagcttatc
     4801 tattttttct ttagattcag acttattgtt tttaatacag aaaactccac caataagtat
     4861 gattaaacaa attggaaggt ttacaaaaaa tagccacttc caattttgag ttccaaagat
     4921 acttagaatt gtgctaccaa tgcttgaacc taaaatattg gcaataccat atgttgcccc
     4981 aactaaacct aaagccatgc ctcttttatt ttctgggaag gtagttccaa attctgctgt
     5041 agcaattggc atgattccac caccaccaat tgcttgaata acacgaccaa ttaataaaat
     5101 atagaaattg gaaaatagtg atgataatcc acatattaat gaaccactac caaatagaaa
     5161 tatactaact agatacacat attttcttcc tattttatct gctaattttc cagagatagg
     5221 aattacagaa gcgtatgtta atgtgtatat ggtaatcatc caaattcccg ttttttcatt
     5281 tactccaagg ctattttgta taatcgttct tgctggtgat actattccag tatcaatagc
     5341 tcccataaat atacctaata gataaagtgt aagtactaaa aatatttttg gattactgtc
     5401 cttgttctta atactagtat tatgattcaa ggtaaacacc accttaatgt attaattttt
     5461 caatataggg ttttaaatct tcaaattcta tatgaagata tgttgaatat gaaaaaaaca
     5521 tttttattat gatattgcat actaatttgt cattattaaa aaaactttct ttttctaaag
     5581 cttcatatac tttatttgaa agctgttcat gaaagtcgtc tttgtgtttt ttcatttcaa
     5641 aaaatctctc tgtttcaaaa gtgtttaaaa agtcattgta ttggatatta tgaactttgt
     5701 ttataaaatt tcttatttca gtaaaaataa tgtttagttt ttttatgtaa tcttcagtgt
     5761 ttttattgga gtattcaatt cgccttatca ataaatccca ttcagatttt gtgatatatt
     5821 ctattatttc ttgttttgaa ttaaagtaat tatatactgt acctgcactt ataccacacc
     5881 tttgagctat tttccttatg ctaatattcg agtaatcttc ttctaaaatc atatcccgag
     5941 atacttttaa tatatcttct tttacatttt ctaaaatctt tggcatatta ccacctcttt
     6001 tattaaaatt attatgaaca ctgtttatta tactccttta aaatttatta atcaatatgt
     6061 aagctataat gtataatatt attatataga tatgatagaa aataagtgtc tttttataaa
     6121 tatctattat attaaatttg tacttaagta tgattattta ttgaaatatg tgatattaaa
     6181 tttttctttt taataaatta aatattatat gataaggaga atatggtgat ggaaaagaag
     6241 tatgtaatgg cattagacca aggaacaacc agttcaagag ctatactatt taataaaaaa
     6301 ggcgaaatag tgaaaattgc acaaaaagag ttcaatcaaa tttatccaaa agctggatgg
     6361 gtagagcatg accctatgga aatatggggg tcacaaagtg gtgtaatgag agaagttata
     6421 gaaacagcag gtataagacc agaagaaatt gcttcaatag ggataactaa tcaaagagag
     6481 actacggttg tttggagtag gtatactggt aaacctattt ataacgcaat agtatggcag
     6541 tgtaggagaa catcagaaat atgtgatgaa cttaaaaata aaggattgga agaatcaata
     6601 aaagaaaaaa caggtctttt aatagatgca tacttttcag caacaaaagt aaaatggata
     6661 ttagataatg tagaaggagc tagggaaaaa gcagaacaag gagagttatt atttggaact
     6721 atagatacat ggttaatatg gaatttaact cgtgggaaag tacatgtaac agattactca
     6781 aatgcatcta gaacaatgat gtacaatata aatgatttag aatgggatga ggatatatta
     6841 cgagaattgg acataccaat aagcatgttg ccattagtaa aaccttctag ctatgtttat
     6901 ggtcatacag atgagcgcat gttatcagga gctaaaatac ctattgcagg atgtgctgga
     6961 gaccaacaag cagcattatt tggtcaaaat tgtgttgaag aaggtactgc aaaaaatact
     7021 tatggaacgg gttgtttctt acttatgaat acaggcagta acatagttaa atctaagcat
     7081 gggttactta caacaattgc ttggggagtt gatggaaaag taacatatgc attagaagga
     7141 agtatattta ttggaggagc atctgttcaa tggcttagag atgaattaaa gataattgaa
     7201 tctgcaaaag atagtgaaat gtatgcaaat agggtagagg atactaatgg tgtatatgta
     7261 gtgccagcat ttacaggtct tggagctcca tattgggata tgtatgctag gggttcaatt
     7321 ttaggactta caagaggagc aaaaaaagaa catatagtaa gagccacatt ggaatcaatt
     7381 gcttatcaga caaaggatgt tttagaagca atgcaaaacg attcaaaatt aaaactaaaa
     7441 tcattaaaag ttgatggtgg agcaagtaat aataattttt taatgcaatt tcaatcagat
     7501 atattaaatg tagatataga tagacccaaa atagtagaaa ctacagcttt aggagctgct
     7561 tatttagcag ggttgtctgt tggattctat attggaagaa atgaaattac ttcaaagtgg
     7621 tcagtagaaa aagagtttaa tccaaatatg agtgaagaga agagatgtaa attatataaa
     7681 ggatggaaaa aagctgtatc gagagcttta agctgggaaa aagaagatga attggatatt
     7741 taaaaattaa tcaaagttaa atttatttga atttaaaaag agctttatgt aaaatatgaa
     7801 gctctttttt tgttgttaat tttgcatttt tatataatca tactaaatta tataatttat
     7861 ttttcgtttt catataaaaa cattggtata gaattgtaaa tgtgttggtc accaactact
     7921 aagagaatgt ctccttctaa aaattcaata tatggtccag gagatattat aagttcttca
     7981 cctcttttaa ttgcaactat tgtagcacct gtattttgcc aaaatttagt ttgtgcagct
     8041 gtttgaccaa tatatttgca attagaattt atggtgaact gaacgggcac taaaggattg
     8101 attatttcaa gtctgttgga atagtctaaa attttattta tagtatctaa tatttcattt
     8161 tctaaattag atttagtatc tattaaatta taaagtgttg atttatatga tgatatattt
     8221 gtaatatctc tatatttttt tataaaagaa gaagctttgt ctggagatag tacaataaca
     8281 cctttaccct ttatgctttt aacaacgcca atgtcctcta aaattttgac agctcttctt
     8341 atcgtttctg gagatacatt gtatttacca gccaaaacag agcgtccaaa tagtactgaa
     8401 tcctcttgta tttcacccgt gtatatttta tttgcaatat ccaacgctat tttttggtat
     8461 attggcatcg tatagttttt atccattgtt tttcccccaa tacaaaattt atgttaaaaa
     8521 aatatataat cttaacaata ctattgtata atttataggt ttatatgtct acaatatgta
     8581 caaatattgt gcatgtttta aaataatatt ttaacatgca tttagtatac atgattttat
     8641 tatacttaaa actatataaa gaattaatta taaaaatata aagatatttt acttacaaag
     8701 aatgataatt atatagatga aagaaacaaa taaatgaaga aaaatgattc tgtcttgcat
     8761 ataaagtgat taaaaatgaa aaatacttta aaaatgaagg ttaaggccct tattttaagt
     8821 gaaaataatt aattattaac taaaaaatgg taaaaagatt ttttgaaaaa gaaatacaaa
     8881 tgaatgttca attaaaataa ttgcaaaaat attccttaaa tggtaaaatt atattaatta
     8941 aatgcaatag aatagttgca aaaataaaat tgaatacaag tgataaaata tttcatgcaa
     9001 tatataaaat aaacaaaatt taaaaattaa aactacttat tctatcaaaa tgttagcaag
     9061 gagggggtca taaattgtat aaagtagatt taaatagtga tttaggagaa agttttggaa
     9121 cgtataagat tggtttagat gaagaagttt taaaatatat ttcatcagct aatatagctt
     9181 gtgggtttca tgcaggtgac cctagtcata tggaaaaaac agtacagcta gctaaaaaaa
     9241 atggagtgaa aataggcgca catccaggtt tcctagattt aataggattt ggaagacgtg
     9301 agatgaaaat aacaaagcag gaagctaaag attatacaaa atatcaattg ggtgctttaa
     9361 tggcatttgc aagttcaaat ggatgtaata tacaacatgt aaaaccacat ggagcattat
     9421 acaatatggc agctaaagat aaagagttag ctatggggat ttgtgaagct atatatgaag
     9481 tcgataaaga tattatactg ttggggctat ataatagtga aatgataaat tctgctaaag
     9541 aaattggact taggtttgca aatgaagtat ttgcagatag agcttatgat aataatggtt
     9601 ttttggttcc aagaagtgta gaaggagcag ttatacatga tacaaaacat gcaatagata
     9661 gggttgttag gatggtaaaa gaaggaactg tagaaacttt aacaggtgaa gttatacata
     9721 tcaaagcaga ttctatatgt gtacatgggg acaatccaaa agcaatagag tttgtcaaag
     9781 aaataagaaa gcgttttgaa ttagaaggta tagaagtttg tccattagaa aacatagaag
     9841 tttgttcgtc agagaacata gtataagact gataacatga gggggagata aaatatgagt
     9901 acaaaaaata ttacagataa aactaaaaat aaaaaagatg tcggagcact tataggggct
     9961 gcatttataa tggcaacatc agcaattgga ccagggtttt taactcagac tgcacaattt
    10021 acccaagatt ttggaccaaa tttttcattt gtaattttaa ttacaacaat cctttttata
    10081 ggggctcaag taaatgtatg gagagtaatt ggtgtatcag gacttagagg tcaggatata
    10141 gcaaataaaa taataccggg attaggttat ttagtagctt ttttagtagc tcttggagga
    10201 ttagctttta atataggaaa tgttggtgga gctgcacttg gtatgaatgt aatgtttaat
    10261 atgaatatga cattaggaac tgtattaagt ggattaatag ctatatttgt atttatgtca
    10321 aaaaattcaa attctctagt cgataagata actaaatttt tagcattagg aatgataatt
    10381 atagttggat atgtagcaat atcaaatcat cctcctgttg gagaggcagt atcaagaatg
    10441 gtaaaaccag aaaatccaaa aggattgata ttcccaataa taactctact tggaggaagt
    10501 gttggaggtt atataacttt tgctggagga catagattaa tagacggtgg aataacagga
    10561 gaagaaaata taaaagaaat aactaaatct tctcttttgg gtatattagt tgccacaatg
    10621 atgagagttc ttttattttt agcaatactg gcagtggttt caaaaggtct tcaacttgac
    10681 ccagaaaatc cagctgcttc agcttttaaa tttagtgcag gagcaatagg atataaattc
    10741 tttggacttg tattatggtc tgctgcaatt acatcagtta taggagctgc ttatacatca
    10801 gtttctttct taaagacact aaatcctttt atagataaat atgaaaaata ttttataata
    10861 gcatttatag caatatcaac acttataatg gcatttatag gaaaaccagc tactttatta
    10921 attttagcag gagctttaaa tggattgata cttccaatca cattaggaat tatgcttata
    10981 gcatctaaaa gaaaagatat tgttggagat tacaagcatc ctacatggct tttaatattt
    11041 ggattaatag tagttttaat atctgcatat acaggaataa cttctcttag cagcctaggt
    11101 gctttatttg cttaatttag aagaggtgac atttatggaa actagatact tgatatcagg
    11161 ggataaggca gtggttgctg aatttggaaa tgagatatca gaagatataa acaaaaaagt
    11221 aatttcattt atgagagcaa tagaaatatc gaatcttaaa ggtattgtaa cagaaatggt
    11281 accaacatat aggtcactta tgataagtta taatccatta gaaatagatt ttgatagttt
    11341 gattgagaat ttaaaaaaaa tagaagataa tttggaaagt atagtattac caaaacctaa
    11401 aatacatgag attccagtat gttatgatga ggtttttggt atagatatta aaaacgttgc
    11461 aagctacaat aatctcactg tagatgaagt tataaaaata cacacaagca gagagtactt
    11521 gatatatatg ctagggttta ctcctggatt tccatatcta ggtggtatgg atgaaagaat
    11581 agctacacct agattagaag tgccaaggac taaaatttat ggtgggagtg ttggaatagc
    11641 aggttcccag actggtgtat atcctattga tagtcctggt ggatggcaaa taattggtag
    11701 gacaccttta aaattatatg atgaaaatag agaagaacag attttgctta gagctggaga
    11761 ctttataaaa tttgttccaa taacattaga tgaatttata gaaatcgaaa aaaataactt
    11821 aagtatttaa atatcgttat agtacaggga gtggtatata tgggattcaa agttgaatta
    11881 ggaggtttcc aaacattaat acaagacaga ggtagagttg gctatggtca atatggtgta
    11941 tctggatgtg gagcaatgga tgagtatgca catagagtag gaaatatatt agtaggaaat
    12001 tctgaagatg aggcttcttt agaagtttta atgttaggtc ctacaattac ctttgatgaa
    12061 tatacgcaaa tagcagtaac tgggggagat ttaggggcta aaataaatgg aaaagaaatt
    12121 caaaactgga ggtcatatca aataaatcca ggtgatgtat tatcatttag aggtgtgaaa
    12181 tcaggtgcta gagcctatgt gtcaatagct ggtggaatag atgttccact tgcaatggga
    12241 agtaagtcaa cttatacaag atctaaaatt ggcggatttg aaggtagagc acttaaaaaa
    12301 ggagattata taaatacttt tattcaggaa aaagatttta caataaataa aaaattaaat
    12361 tcaaaatata ttccaactta tagttcagaa atcgtactaa gaattgtaaa aggtcctcaa
    12421 tttgatgctt tttctaacgg tgaagtagag aaatttttat caaataaata caaagttacg
    12481 aatgaaatag atagaatggg ttgtagacta gatggagaga gtataaagca ccttaatgga
    12541 gcagatatta tatctgatgg tatttcttat ggagctatac aagtacctgg tcatggaaaa
    12601 ccgattataa tgttatcaga tagacagact agtggtggct atactaagat agggaacgtt
    12661 ataagtgtag atttgtataa gttagcacaa gcaaagccaa atgatgtagt aaaatttgaa
    12721 cttgtagata tatatgaagc acatagacta ttgagagagc aagaagataa gattcaagat
    12781 atctacaaga gtatgaaaaa tataagagtt gtaaaggcaa aagtattaaa tgatatagca
    12841 gtttaattta attttaacaa atacaatttt tattataatt tataatataa cttacaaata
    12901 gctatttact atttaccata attaagatgg tttagattag tcaaatagct atttttttag
    12961 tttgagtaaa tattttaaat ttaaataaat acttcaattt taaaataaaa gcaatattta
    13021 aatttattat attatctaca aaaagtaggt gtgttatgtt gagtttattt agattttatt
    13081 ggccaaattg gaggtgatat aactccatat ccacgagcta ttgtatcttt acttggattg
    13141 atgattttat gtggtacatt agcttttata aatgttgagt cacctggatt taatctaact
    13201 accttttcac ccatacaaat atcaaccaca ccactcgtta ccaccataat ttcttctgat
    13261 gcatgataga tataatcttt agcatcctca ctttctggtt taagttcaaa ctctacaaat
    13321 aaaattgaag gtgtaaattc agttgtaggc attggagata ctatctcgta aaacatttca
    13381 ctctttggaa atttcatcat aactctatct tcttttctaa ttactaaatc atcagtatta
    13441 ttgtcatcca taaacatata taaaggagtg tctaaaactt cagcgatttt tctcaatgaa
    13501 gacaaggatg gttcaatagc atctctttct atttgagaga tataacttat agataaatct
    13561 gtcaatttgg aaacatcact taatgttttt ttcttgcttt ttcttatatt tctcatcttt
    13621 tcacctagca taagaacctc caaataataa aattaatata aaatattcgc ttaaaacaaa
    13681 gtaattatag cacaacatag aagtagtggc aataagaata catagtagat tttgacaatt
    13741 aaaatttaat taaatccaaa taaaagtcta tgaaaatgaa ttattgttaa attaatataa
    13801 agaaaataat aataaaaaaa tttaatagaa gagtattaat tgaattaaga ttatcacatt
    13861 atattttgga aaagagtaaa ttttcagtaa attatcttga catatatatt gaagtaataa
    13921 tataatttaa agtataaaat tttaaaaatt gaattataac ttcaaaaaat aaggagggta
    13981 atatgaagat aacagatatc aaatttgaaa agcttagaat taaattaaaa aaacctgtag
    14041 tagtttcatt tggagtaatt gaatatggag aaagtgtaat tttaaaaatt gaaactgatg
    14101 aagggtattg tggatttggc gaagcagcac ctcttgcagc agttacaggt gaagttttgg
    14161 ataatgttct ttcagtattg cttatgttta aaaaagagtt aataggcaaa gacccactag
    14221 atattgaaac catacataca atcatggatg gaattattat aggtaatact tctgcaaaag
    14281 cagctattga catagcttta tatgatatta aaggaaaaat tatgaatgca cctctttata
    14341 aagttcttgg aggatttgat agtaaagtcc aaacggatat tactataagt atagataagc
    14401 cagaaaaaat ggcagtagaa gctttagaaa gagtaaaaga aggttttagg atattgaagc
    14461 ttaaagcagg tataaatcct gaagacgata ttgaagctgt aaaattaatt agagaagccg
    14521 ttggagagag tataagaatt agaattgatg caaatcaagg atggaacgta aatagcagta
    14581 taaatactat caaaaaatta gaagaattta atgttgatgc aattgaacaa gcacttccac
    14641 attgggattt agatggtact gcttacataa gaaataaaag taatacaaaa attatgatag
    14701 atgaatcgtt acattcacca atagatgcaa ttaaggctat aaaaaagaat gcagttgata
    14761 catttaatat taagctgatg aaatcaagtg gaatatatcc tgctataaaa atcaacaaca
    14821 tagctgaggc aagtggagta aattgtatgt taggatgtat gctggaaacg agaattggta
    14881 taacagcagc agcaaattta atagcatcta aaaagaacat aacagaagct gaccttgata
    14941 gttttatgtt ttgtgaagaa tcaaaagata tatctggagg atttgtaatg gatggagata
    15001 ttatgaatct tgtcaataaa ccaggtttag gaatagaagt aaacttatga taaatttgga
    15061 ggttaaatat gaatacactt ttttcaaatc caggtagtat acttgctgta atgacatcaa
    15121 tgattgcatt gggtttttac ttacaaagat acaaagccat taaaagctta ggaccagcac
    15181 ttacaataat cataatggga ataatacttt caaacttaaa agtagttcca gtaagtactg
    15241 agttatatgg aactatttca acatatgcta tcccagtttc tatgactata atgttgatga
    15301 gtgttgactt aaaagaaatg acaaaattat caagagaacc tttaattgca atttttgtag
    15361 ctgtattaac tgtaagtatc atggcatttt tatttggatt agtatttgca gaaaaaatat
    15421 cagaaggttg gaaggttgct ggaatgtttg taggtacata cactggtgga agtgctaatc
    15481 ttacagctat tggaacaggc cttaatgtaa gtagacaaac tcttgctgca gcaaatgcag
    15541 cagactatgt aattggagta ccaacattga tatttatgtt tgctctacca gcaatattaa
    15601 aaaattcaaa gaagtttaaa aaactttggc catatcatgt agaagaaaca gaattagaag
    15661 attgtcaaaa tgaagaattt atggagtcaa aagaatggag tattaaagat atagcttgga
    15721 tgttggctat aggttttgtt gttacagaag tttcaacaat acttgctgga tatttcaatt
    15781 caagttttag tagtgcagca agaatacttt tagttacaac aatttcaatt ataattgctc
    15841 aattaaagcc agtaaagaaa ctaaagggaa atttagattt aggattattt gttgcattat
    15901 tctttttatg tacaataggt ttttcagtag atataaaaga gtttttaggt tcaactttta
    15961 ctataacact ttactgtttt agcataattt ttgcttcatt tgtttttcat ttaggcataa
    16021 ctagactttt aaagataaag tatcaatatg taatactttc tatagtagga gcaattgctg
    16081 atggtcctac ttcagcttta gtagctgctt ctgcaaagtg gaattcgctt gtaagtgtag
    16141 cagtcgtaat gggagtaatt ggtggagtat taggaaatta tgcaggtata tcagtggcat
    16201 atgcaataaa gatgtctcta ggattatagg ggggagttta attgataaaa tttaaattat
    16261 acatatctgg actatacagt ggaaatgtaa tctttgatgg cgatttatta attgaaaagt
    16321 tgaatccttt tacaaataaa atagaaagtt taaaaccaat aagcaaagaa gaaaatacat
    16381 actatttaaa tcttacaaaa atagatttaa agtctttatt taataacttt gatgtatata
    16441 ctaaaagctt agtaaacaca gataaagaca cagttataaa taacttagga gaaacgcatt
    16501 ctaaacttaa tgaatatata tgggttcaaa gaaacaaaaa gttcccacta gatataatta
    16561 ttgtagataa taaaatagta ggttttatct gtttatccag agaaacttgt actatattaa
    16621 ttatggatgg atatgaagag tatactgtat taaaagaatg ggaaaaaacc cataaaaatg
    16681 aagagatata ttctataaga tttggtggaa attatatgat tgatatgaaa gatggtataa
    16741 aactttctac agatgtatat ctccctgatt ttgtagattc taccaaaaaa gcaccaacaa
    16801 ttcttatgag aactccatat ggaaaagaaa atgataaaga aatatattat aaatatgtac
    16861 aaagaggata tgctgttgta atacaagatg ttaggggaag aaatgaatca gaaggtaagt
    16921 gggaacctat gattcatgag agggaagatg gagatagtac cataaattgg atagtatcac
    16981 aggaatggtc aagtggaata gttggaatgc ttggagcatc atatcttggt tatgtccaat
    17041 gggcagctgc atcttcaggc aataagcatc ttaaagcttt agttagtata gtaacatcag
    17101 gaagcccttt tattgatata ccaagaaaag gaggagcttt tgtatctggt atgcttgcat
    17161 gggcatttat ggtatcaaga aataaagttg atagaagtaa aatggtaaga gatgattggg
    17221 atgatgtttt aaacatacga ccaatagaaa atatacctgt tgaagctctt ggatatagaa
    17281 ttgaatttct ggaagaatgg ttaaagagag tagaaaaaga tgagtattgg gatttgatgg
    17341 attggcattt acaaaaagat aaaataaatg tacctgcatt ggttgtatct gggtggtatg
    17401 atgataatag tatgggaact acagaggcct tagatgttat aaaggactat gaaaaaggaa
    17461 agagaaaggc tattctaggt ccatggatgc ataattcaaa tactttaaga gatataaatg
    17521 gtatttcttt aggaaatagt tcattaagat atgatttgga ttataattat ctattatggt
    17581 ttgataaata cttaaaaggt atagaaaata acatagatac gactgctcca gtagagtatt
    17641 actctgtagg ttttaataag tggaagacag aagaaaattg gccaataata aataagatag
    17701 ataaaagtat gtatctaata agtgatggaa atgctaacac atctttagga aatggtagat
    17761 tagtatttga taatgattta gaagaaaagt atgatagtta tatatataat ccaaaagacc
    17821 catcagtaca actaattgat atgtcagaga atgaagttgg agttccaaat aactacaaag
    17881 atttagaaaa aagaagtgat atgttgtgtt atacatcaga tgcttttagt gaagaattta
    17941 ctgtaacagg tgatataaag ttagagtttt ttgcatcaag ttctgccaaa gatactgact
    18001 gggttataaa aattatggac gtggatttag atggaaattc aattaaatta gctgatggta
    18061 tacttagtgc tagatttaga aatagttttt ataagtcaga gtttatggaa gaaggagaga
    18121 tttataagtt tacgattata acttcaaaaa tatcaaacac gtttaaagtt ggtcataaaa
    18181 ttagactaga tataacgtct agtgctaaaa actttatttt ccaaaatagc aacacgaaag
    18241 agggatataa tagtattgaa tatatagaag caaagaatac gatttaccat ggaggtaaat
    18301 atccatctaa attgatattg ccaatagaaa ataagtaaat ttatatagat taaatagtga
    18361 ggaggtgtct caaaatgaat tttttagttc gtgagacact ttcttttata cgaaatcaaa
    18421 tatattttca tcatttcaac aatgaaaaag aggcttatct ctctgttttg agatagcctc
    18481 ctataagcta aattgattat tttgtagctg caacaacgct catttcaact aacaaatgct
    18541 ctctagcaag tctagcttca acacaagctc tagcaggttc aaatcctttt tcaacccaag
    18601 catcccaaac agagttcata gcttcaaagt ctttcatatc tcttaaataa atagtaacag
    18661 atagcaaatg ttgcttatct gaaccatatt tgtttaataa atcttctatt ttagctaaaa
    18721 cttcagttgt ttgttcaatt actcctcctt cagcatgagt ttgtccacat aaataaactg
    18781 tattgttatg aactacagct ctactcattc ttccagttcc ttcatatctc tttatttcca
    18841 tattttaaat acctccatag ttaagaaatc tattaaaaat ctaaataata aattaaaaaa
    18901 agtctatact atataaaatt atattatata ttctgataat aatccataaa aacatataac
    18961 tttttagcta taattattat tgttattaat tgaaaagact tattagttac ttgacatagt
    19021 ttacactttt aattatagta taaattggaa tatttagata attattattt aatgtataag
    19081 ctaagttttt aattaatatg gctttataca tttatataga aaagaggtac gtatgagtag
    19141 cacaatcaaa tcaaatcgaa taaaaggtat aatgtttata atagcatcag cgcttggctt
    19201 tgcaatgatg tctgcattcg taaagctagc tggagattta ccaagttttc aaaaagtgtt
    19261 ctttagaaat ctggtatcag ctataatagc tttttggctt atcataaaac ataaaggaag
    19321 tctaactggt aaaaaagaaa atttaaaagt acttttatat aggtcaatat ttggaactct
    19381 aggagttata tttaactact atgcaattga caagttgatg ctttcagatg ctaatatgct
    19441 aaataaaatg agtcccttcc ttgtagtaat attttgtgca attgctctaa aagaaaaaat
    19501 aaatgtaaag cagataggag ctattataat tgcatttata ggagctttat tcataataaa
    19561 accaacattt agtgtagaag taattccata tcttggggga attgcaggag ctgtatttgc
    19621 agcaatggca tatacttgtt taagagttct tggagataga gaagattact atacaattgt
    19681 atttttcttt tcagtttttt cattagttac tgtagggcca attgcttttg cagtatatga
    19741 accaatgacc cttatgcaac ttatatatct attactaggt ggagtatttg caagtctagg
    19801 tcaatttgga ataactttag catataaata tgcacctgct aaggaaatct ctatatttga
    19861 ctatagtaat ataatctttt cagcaatttt aagtatattc ttattcaatg tctatccaga
    19921 tatgttaagt gttgttggat atttaatagt attttcggca gcattttata tgtttttata
    19981 caataagaag ttagataaat tagacaaaga aaagaagaag taaactagat aaaagtgttt
    20041 ttataatttg tattgaatat ttaagtcttt gtattaaaat taaagaaaat ttaattaaaa
    20101 atgtttggta tattaactta taacagcagt taaaattgaa aaattataac tgctgtttct
    20161 tttattaaaa atatccaaat gtctgtaaaa atagactata atagagtagt ataaataata
    20221 taaaggggat aaattaaagt atggaaaaag tgaaagttgc tgtgattgga gcaggtaata
    20281 gaggaacgta tgcatatgct ccatatattt atgaaaattc tgatatatgt gaaatagttg
    20341 ctgtagcaga acctaaaaaa ggaagacgag aactatttac acaaaaatat aacctagata
    20401 gtaaaaatgt atttgaaact ttagaagact tttttaaaca tgacaagatg gcagatgcag
    20461 taataatagc tacaaatgat gatagacatt atgatgtagc aaaactagct cttgaaaaag
    20521 gttatcatgt actattagaa aagccaatgt ctaatagttt agatggtttg gtacatatag
    20581 atgacttgtg tgataagtat aaagataaga tttttatgat ttgccatgta ctgagatatt
    20641 ctccatttta taataaatta aaagaaattg tagaaagcaa aaaattagga gagctagtta
    20701 gtatacaata taatgaaaat ataggatatt ggcattttgc acatagcttt actagaggga
    20761 attggagaaa tagcaatgaa accagccctt taattttagc aaagagttgt catgacatgg
    20821 atattttgtt atatttagta ggaagtagat gcaaaaaaat atctgcattt ggaagcttaa
    20881 agcatcttaa taaccaaaat gctagtggag agatggcaca aaattgtctt cagtgtttag
    20941 ttgaaaaaaa atgtccatat tcagcaaaga gaatttattt agaaaaagat agaagtataa
    21001 atagagctgt tcacataaat ccaactgaag agaatctatt aaatatactt aagacaagtc
    21061 catatggaag atgtgtttac agatgtgata ataatgttgt agacaatatg gtaaatatat
    21121 tacagtttga aaatggagtg acggctactt ttaatttatg tgcatttact aaagaaaatg
    21181 gaagaactat aaagttaatg tttagtcatg gtgaagttgg tggggattta aataaaaatg
    21241 agataagaat taaaaaattt ggaaaaaacg aagaaatagt tatgaatcca tcaaatcaac
    21301 agaatatggt agaggaatat gatagaaatc ttatagctga atttattaaa ttagtttcaa
    21361 ataaagaact agaaaagggt agagtagcag caaaagaagc tatacaaagt catgtaatgg
    21421 catttgcagc agaatattct agggtcagtg atgaagtagt gtatatagaa gaattttttg
    21481 atagtgctaa gcaaatgact aaggagatag aagaaactat attttaatta ataaaaattt
    21541 attaaataaa ataaagatag attaaataat agaaatacga aaatatgtgt tctattaagg
    21601 aatctatctt ttttattaag gttttttctt atccctatca aaaaaatcca tacattctga
    21661 taaaagataa gaaagtaaag cgaataagat ttctgtactt aaaggaatcg ttactgaatc
    21721 cataaaggta tcaattatgt tcatagctag aaaagttagt ataaaatcaa ttataaattc
    21781 gcaaagttta tatattgagt atggtaattt gtttacatat ctgaatatat ctaaaatagt
    21841 agtgcataga aaatcaattg gagtggttat acaaaaatat attaaaaaga aaattaatac
    21901 agctccaaga gatttatatt caaatcctaa gaatgataag aagccaattc ctataaccgt
    21961 gaaaatacaa aaagttatac caaaaaaaac agcaaaaaat aaaaatacag aaataaattc
    22021 gcctaaagga atccttcttt tattacttat cttaatcaac tccttaattt atgatatgaa
    22081 atttatataa ataataacat tcttaaaaat tcataatagt cctctgtttc aaatagtact
    22141 gtttgagaat ctattttttt catatttgga taaaaagagg ccttaaaagc tttataatgt
    22201 tctctaaatt ttatttctat cttaaattta tctggcaatt ttattaagtg cctagataaa
    22261 tctccagata aagctttttt gacacctttt tcaatttttt ttagtgcaag gtttggatgg
    22321 attgaaattg aaccatttcc aattcctttt gacacaggaa cagtgactat atttggattt
    22381 agtttttttg cactctcaca aagcatttca tctccactta aaaatgctac aggaacatta
    22441 taatatgcag caatataagc atttatagta aattcagatg ctatttcatc atttatttta
    22501 aaatagtcat aatcaatatc catagtatga gaaagtggat taccattaca agtagctcca
    22561 gagtgatagc cggtgaatat acatgcatca aagctttcat caattccagc catcataact
    22621 agtgggtctt tggtccatcc tctaagtatt ttgacgtttt caggtaaaat atttggatta
    22681 atatttctac cactgtcatg tgcatcttta acaagtattt catgtacacc agattgagta
    22741 gctccatcac atgctgctgt tatctcttta ctcatttgtg ttctatagtg ttgtgaaaat
    22801 tcacattcaa gttttgtttc atcccaattt acaataccac aagtaccttc tatatcagca
    22861 cttaaataca atttcataat atataccccc ttatgtttat tattgatgat aataacagat
    22921 aacagaaaat acataatcaa taaagggcat aaaaatatta tgtcttacta ttaattatta
    22981 ttaaatctgt aactgaaaaa taactaaatt ggtattaaaa aatataacac tatgtgtcat
    23041 ttatttatat agttttttat atctgatgtt tatataaaat tgtaacatat aagtggttac
    23101 atttactagt ttaacatagt taaaatagtt aggtattatt tttaagttaa attgattatt
    23161 ataaaatcgt agaataaaag aaaaatttaa tttattaaaa tgcatgaaat gttttttcct
    23221 caacataatg ctaaattaaa ctcaaaaaga aataatagaa aaatcttatg tagcttagaa
    23281 agaatgtgaa tgatttaagg gaaaaataga aatttattat ataatattag gctgtattat
    23341 gtaatacagc ctaatattat atgtatatgt gacataaaat cttggtaagt gtaaataaaa
    23401 taaatgtgga aaacaaatta atgaaatcaa gaaaaaagta atttaaaatt caatttcatc
    23461 ttcccaatta aattctcctg atttttctac actcttaggc cagtgactac accatttagg
    23521 aacggacgga ttttcaaccc tatcaacgct tggagtatat ggctttaagt ctataacagg
    23581 actattatca ttagcatcaa tatatggtat ttttattata ccattttcat ggtcgatatt
    23641 gataatttgt acagcagtca atgctattgg attgggtctt ataggtgacc ttgttgcaaa
    23701 tattcctata acgtttggtc cacttttata aggttttggg gtttctaaaa tacttcttag
    23761 ttcaggatta tcaaacccat cagcccacca aaatacattt aaatgactga aatcgtcgag
    23821 ttcatttaat gcaggtacat actttttgtc taatttaata aagacctctt cgccttgaat
    23881 acatatttta cctataggat ttattaatat ttctttcata atattacctc caactattta
    23941 atttatcatt tgtaatttta gggtataaca tgacatcatg tgagggtcaa tataaaataa
    24001 taaataaaaa tattaatagt tagaatgata atacattttt tacatggagc atgacaccag
    24061 taataaggca atcttcgtca atatcaaaag tagaggtatg caaaggggaa gtaatgtttt
    24121 tttcacgatt tgcacaacca aggtggtaaa aagctccttc acagtgtctt agaaaatacg
    24181 aaaagtcttc agccccaaga gtgggcgagt ctttaaggat aatattttct tctccaaaca
    24241 attctacagc attttgcttg acgtaatcta ctagatggtt tgaattgata agagctggat
    24301 aaccttcctc aatttcaaca gaaatacttc ctccaaatgt tttacaagta aaatcgcaaa
    24361 tttctctaat tcgttttttt gaaaactctc tagtttcagg agtcaaggtt ctcaaagtac
    24421 cccttataac cactttgtca caaactatat tttccttgat tccaccttca atctttccta
    24481 gagaaataac cacagaatca gttggatttg tgtttcgact aacgattgtc tgtagagctg
    24541 taataatatg agcagatata acaatagcat caataccttg atgtggataa gctccatgac
    24601 attttgaacc atgtacagtt aattgaagtg tatcggtgga agcatttaga gtgttgtatt
    24661 ttaattcaat tagatttttg ttgatattgg gatttacatg gagtccaaat atatatttta
    24721 catttgggtt ttcaagacat ccatcttcta tcataagttg agccccacca atagtttcct
    24781 cggcaggttg aaaaaagaat tttacattta catttaagta attctttaat ttaaatagta
    24841 tattacaagt acccagtaaa atagcagtgt gtgcatcatg accacatgca tgcattttac
    24901 caatattaat agatttataa gaataattaa gttcttctgt tataggtaag gcatctatat
    24961 cagctcttat agcaactgtt tcaaagtttg gactcacgtt aatgtatgca gttatccctg
    25021 tatgattctt atattcaata taatttatac ctatttcatt taagtattta ataatttttt
    25081 tcttagtttg aaattcttct aatccaagtt ctggagtctt atgtaaatct cttcttatat
    25141 ttataatcca atcatttatc tcatgacaaa gagttcttat attaagcata aaacacatcc
    25201 taactatatt aaaaatttat ttttcttcat taattatatt attttatgta aatcattatt
    25261 atgtatcaag tacaaataaa atatcttata attatattag agatatttag tatggagaag
    25321 aatatatcat tttataagaa gaggtgttta aatgttagga ataattgcgc catctgggcc
    25381 attaagaaat acaagtttgg aggaaataaa gtttaattta gaaagttatg gttacgaagt
    25441 taagttttct gaaagttgta gtttaaatta taaaggttat ttagctggta atgatgatat
    25501 tagagcaagg gatatagaag atatgttttt agataaagat gtagatataa ttatgtgctt
    25561 aagaggaggt tatggaacaa caagaatatt ggataaaata aattatgata taataaagca
    25621 aaatccaaaa ccatttatag gtttttctga tataacaggt ttaaatttag ctttttataa
    25681 aaattgtgga cttttaccat atcatggaat aatggcagca aatgtaggta aatgggataa
    25741 atttacctat aaatcattgg taaatgcttt agaatttaaa gatgacttat atttagaaaa
    25801 tccaaaagaa gaaaagattt atactgtatg tgaaggtaaa gcagagggaa taataatggg
    25861 aggaaattta tccttaataa ttgccacaat gggaacaaaa tatgaaatag atgcaaaaga
    25921 taaaattttg tttattgagg aaattggaga acctcaatat aaactggata gaatgttaac
    25981 acaactatat tcttcaggaa aacttgagga atgtaatggt ataatttttg gagattttaa
    26041 ggattgtatt gaagaaaatg atttgatgga attactaata gaatttgcaa ataaggtcaa
    26101 taaaccttct atatataatc ttcagtcagg acattgtatc ccaatgataa ctattccttt
    26161 gggcagaatg tgcgaattag atgctacaga aaaaatcgtt aaattaaaaa aatagtgtgt
    26221 atataaatat aatagaaata tggtataatc ttatacaagt aaaaatttag ctaggaggat
    26281 gaaaaatgtc tgatgataga ataatagact ttaacgaact aaagaacaaa gttaaagatt
    26341 ctgatgtaga taaatttgag caatacatat ataacttata tttttctgtt atggatggaa
    26401 agatgtccat ggctgagttt tcaagaaaaa tattcgatta tatgagagat aacaacatat
    26461 ctcaagaaaa atttatgaaa atacaaaagc aatttatgga gagatatggt atggacactg
    26521 aagaagtaga aaaacaactt agaaactttg gaatcgaccc ttctacagca ggttttatga
    26581 gtaataatac ttccagcaaa gtatctacag aagatttaga gtcatttaag aagagtgcag
    26641 gattttatga gaaatatgga gaaaaaatcc aaccaaagag ttgtataaca acatttataa
    26701 aaaatgactt aaatgatatt aatgtaataa tcgaccaaga aaagataatg ctttgtagtg
    26761 atagaaaaat aaatcttatg gattcagaac taaatgagtt tttattagaa tataagaata
    26821 tgtttaataa aaaaataaag gtagtaatgt gcgaaactac gaataagtat gattattaaa
    26881 aaatgaattt aaatactgaa gtagtatcaa ttaattgtac tacttaaaaa taaaaatcgc
    26941 aatttattat tccttataaa tttgtataag gaatatctaa actgcgattt tttatattgt
    27001 gaagattagt cataatagat agtgttttaa attgtaatct tctcactaaa actgtgtttt
    27061 tctattagaa attttattaa aaataaatat ctgataattg tattatatgg gatgtaaagt
    27121 attagaggct taacgaatct cttttaatac attaactcca ttttgaatca tactgttgaa
    27181 aaatactaag tttaaaatct cagcagggtg aatgttagga atatcataag tatctactaa
    27241 attcataggt acaactatat ttttttcaat attatgttga ttaaaatatg attttagagt
    27301 tattgcaaat tgatatatgc atatatctgt gcaatcgcca actataatta tattatctat
    27361 attatcaaaa tccaaatttt ctaatgcaaa aaatccatta gtagaatttt taggaagaat
    27421 ttgtaggttt tctatcgact taagttcatc aaccaattca ctttctacat cattttccaa
    27481 acagtgaaca ggataactca aaagttctat tgatttagga gtatgagaat cagtaaatgc
    27541 tattactcta tttaatttgt ttgatatttt ttttgtaaac atttcaattg gttttattaa
    27601 agactctact ctaggagagt ataatgcacc ttgtcttgca aagccattat taacatcaat
    27661 tataaataaa gcagttttac tcaaatcata attttctatt ttttctattg gtagattatc
    27721 taaattactc tttaaagctt caagttcatt taataagtta tccatagtta tacctccaag
    27781 ataaatttaa taaaattgta catattaatt atataaaagt caaggagtac aaactattgt
    27841 ataattagct gtattgtttt gagtgtaaaa gatttgaagc aatatctgga tagttagtaa
    27901 aaataccatc tacattgagt tttagacagt gtagtatatc atcctcttca tttacagtat
    27961 atacattgac accaagtaga tttttgtgac aaagttcaat tagagattca tttactaaaa
    28021 ctagaggtgg atgaaggtaa ctattaccca gaaatttagc aaaattgact acattgttta
    28081 taggtaaata acacaataat cctgttttta cactagggtc taatttatga aagtttttta
    28141 agctattgct gttgaaacta gatataagaa ctctattttt catattatac tttaaaataa
    28201 gctctaaaac gtctttttct atattgggat agtttatttt gtcagttttt agttctatat
    28261 ttaaaataag gtctgtaggt gaaattagaa ttaatacttc ttctaaagtt ggaattttac
    28321 attctttatt gttttgatat ccttcaaaag atgagtttag agtttttaat tttcttaaag
    28381 taaagtcttt tacaaaacca tgtccattaa aagttctatc aaccttttca tcatgaataa
    28441 ctacaatttt tccatcctta gttctgtgga catcaagttc tattccatat atatccatat
    28501 acaaacattt tttaaaagct agaatagtat tttctggata ctttccacta aaccccctat
    28561 gagcataaat attcatttat atggttccct cctataagta taacttatct tataaaaatt
    28621 taattaatat aatatatcat attccaaaag gtattgttat atattagtta aataactatt
    28681 tgtaatttat atattatata aatgtagaaa aataaaatca tatatgtgtt ataatatatt
    28741 ttaaacattg taaatattac gaatttagta gtcataataa gtgtaatttt atgcaagctc
    28801 atgtttttag agcttgcata tgtattatta atatatttat atattgtgaa aataaaattc
    28861 caaaagaatt ttttctatag acttagtagt aagggggaat tttgatatgt atgaagttaa
    28921 tttagaacga gctatgaatt attggatgga tttgttagat gattatacga atatagcaaa
    28981 aattccaaat gatttttttt ataaagattg ttctgatgaa gtagtagatg aggtttttaa
    29041 gatgaatatt gagaaacatg aaaggttaat tcaatattct aaagtgaaca atattagcat
    29101 agactctatc atagaaacta tatatggcct tatacttcaa aagtatatat atgaagatga
    29161 tgtagttttt ggtaaaacaa tgaatataat tccaataaga gtaagagtta gacaaagtga
    29221 aaaatttata gatgttgtaa ggaagctaaa aaggcagtgg agcaggtctt tagagtatgg
    29281 atactatttg ttatcagata tagagaataa aaatcatcta aatactaagc ttataaacac
    29341 aatatttata gctaaaagtt ttgatttatc atatgattat gacatagaaa aaaaaggata
    29401 tgatttatct gtaatagtat atactaagag ggcacttaaa ataaaaataa agtataaacc
    29461 atctatatat aaaagagaaa ctattagaag aattttaaat caatttgatt atataataga
    29521 gcaaataata gaagataatg atattttaat agaagattta gaatttgtat gtaaggaaga
    29581 agaaaaagta ttattggaag actttaataa taatcaatat gaagtaccct atgataaaag
    29641 ttatgttgat ttatttaggg agcaagttaa ggtaacacct agtaatatag ctgctaggga
    29701 tgaaaataga aagttaacat ataaagaatt ggatgaggta actgataaac ttgcaggtta
    29761 tttaaatcat ataggagtgg aaagtgaaga catagtagct gtaatgttac caagagatat
    29821 taacataatt attacagcaa tagggattat gaaatcaggg ggagcatttt ttccaataga
    29881 cacgagtaat ccagaagaaa gattgaatta ccttcttgaa gatagtaatg caaaagtagt
    29941 gataactaca gatgaactca aaagtaaggt tgtaaatgag aataccatag tactcgatat
    30001 aaatgatgaa gaaatgttta agcttggata tgaattaact gaaaaaataa catcatcaaa
    30061 ttgtgcatat acgatatcta cttcagggtc aactggtagg ccaaagacca tagctataga
    30121 acataagagt ttagtaaata tgtgttatta tagcgtaaaa tctataagcg ctacagaaaa
    30181 tgatatatgt ggtatatact taagtttcag ttttgatgct gtaatgaaac aactattccc
    30241 ttatttgctt gtaggtgcat caatagatat tatgcctgaa gaagctaaat ttgatgaata
    30301 tacagtaaat gaatactgtg aggaaaatga cataactata ctagcattgc caacagcatt
    30361 tgcaaggtta tttatacaaa attgcaataa taattcactt agagttgtac agactggagg
    30421 agaaagatta aaaggatata gaaagcgtaa ttatgaacta tataatgagt atggaccaac
    30481 cgaatttact gttgtatcga cttcttttca tgtggacaga gaatatggaa aaataccaat
    30541 aggaaaacct atatttaata cttatgcata tgtattagat aaaaagaaca aactatgtcc
    30601 aattggagca ccaggagagc tatgtctatc aggcattcaa atatcaagag gatatttaaa
    30661 taaaaaaggg ctaacagagc aagtatttgt agagaatcct tacaaaactt gtgaatataa
    30721 taagttaatg tatagaactg gtgatttagt aagatggtta gatgatggaa atttagatta
    30781 tattggaagg atggacaatc aagtaaaaat tgatgagttt aggatagagc tatatgagat
    30841 tgaaaatata ataaataata ttacagaaat taaaagtgtt gtttgtatat cgagaacaaa
    30901 tgatgacggg gatatgtata tatgtgcata ttatgtaatt gatgaggaag atagtggtaa
    30961 gataaatgaa agaaccatta gagaatattt aaatgagcat ctacctccat atatgattcc
    31021 aacaataata atgaggatag ataaaatacc agttacacca ataggaaaag taaataaaaa
    31081 agcactacca gagtgatttt ttattatgac aggtgttgta aagtgattcc atacatttct
    31141 ttatgacacc tatttttctg aggaaatcaa ttgatgtaaa aaaaagaatt ttctgtatat
    31201 atttactatg gtgaaaattt atttataaga atgattattg tattttttat acttttccgt
    31261 gttttaaagc atttttttta caaaattaat tttacattaa aaattttttt tattttatat
    31321 cttaatagtt tttagtagaa ttgaaaaata aaaagaaaat tagcataaaa atatctaaaa
    31381 gtagaaatta ataatatagg gttaaatttt agtagttttg actcgctatt tgtaaaataa
    31441 taaaaagtta agttttatat gtatatatgt tataatatat acaaattgaa aaggggtgaa
    31501 aattttatga acaaaaaatt acctaagggt gcttatggtg aggttagtgg aaaagattac
    31561 gttccatata tcacagataa gtccagaacc ggtggaaatg tagctgtact gattatcggt
    31621 attattttag ctgccatttt tgccgcatct acaacttact ctggaatgaa agccggtctt
    31681 acagttgctg caggtattcc aggagctata ataggttctg cttttgttgg agcatttgct
    31741 cgttcaaagg gtattcttgg taaaaactta atccaaggga tgtcaagtgg tggagaatct
    31801 gttgcaagtg gatttatttt cgtattacca gcagttatat taattggaag ccaaattaca
    31861 ttctttgaag gcctagctgt tggagttggt ggagttttat ttggtatagg aattgctgct
    31921 atagttcaca actatctaat agttgaagaa catggtaaac ttatgtatcc agaatcaatg
    31981 gctatatctg agacacttgt tgcttcagag gctggtggag attcaattaa gtacatggga
    32041 attggatttg taataagtgg atttattact gtattaactg gttcattctt aaatgtagca
    32101 aacaatgtaa tgagtcttgt aggaagcaaa ttctacaaat ggaaatttga tattgaagta
    32161 aatcctctac ttttaggaat tggatttata gtagggcttg aagtttcttt aacaatgttt
    32221 gcaggaagta ttttatcaaa ctttggtata gcacctttaa taggatactt tacagatatg
    32281 gctaaagatg gagctatggt atggaataat cctgcaatgc ctcttaacca aatggatgtc
    32341 ggagcaatat ctagtagtta tgtaaaatat ataggtgctg gtatgatgct ttgtggaggt
    32401 attattggtg caataaaact tataccaact ataatcgctt ctataaaaga aacacttaaa
    32461 gctaaatcaa gtgctggaga aggagaagaa ggttcatcta tacaaatgat acttttatta
    32521 ggtggagtag ttattggatt tttagcagca ttcttaattt ctggtaacat tgttatggca
    32581 attattggag ctatcatatc tcttttatta tctttattat ttgtcatagt agctggtcgt
    32641 ttaacaggaa caattggaac atcaaatctt ccagtttctg gtatgactat agcttctcta
    32701 gttattgtaa cattagtgtt tgttataatg ggatggactg atttagaagc taataaatca
    32761 ttacttttat ttggttcatt tatagttgtt gctattgcta tagctggagg ttatactcaa
    32821 tctcaaaaag taacttatat tattggtggt agcaaaaacg aaatgcaacg ttactttaca
    32881 attgcaagta tagttggtgt tatagttgtt gtaggagtaa ttttattact ttctgaccaa
    32941 cttagagcaa ctggtgataa tgtacaattt gcattaccac aagcaaactt aatgtcaaca
    33001 ttaacttctg gtattatgtc aggtagttta ccttgggtta tgattattgt tggagtattt
    33061 atggcaatag ttctatatgc attaaattta ccaataatga ctatagctat aggattctat
    33121 ttaccaattg caacaacttc tataatatta gttggagcat taattcgttt atttgttgaa
    33181 ttagtatcta aaactgaaaa agaaaaagaa gttaaagttt ctaatggtat aagtttatct
    33241 tctggtcttg ttgctggtgg ttctatcatt ggtttaatag gtataatatt acaagtaact
    33301 ggagttgtaa ctccaaaggt accaagtgga tttgctgcaa caaattcaat ggcaattgct
    33361 ttattagtag ttttagttgt gcttacaaca ttacctattg ttttatctaa agtaaaaaat
    33421 aatgagcaag agtaatgatg ttttagatat tgtatttaaa attactgatg gacttgaata
    33481 tgaagtagct gaaaatagtg ttgttactat acttgaaaaa caggaccata aaattcaaaa
    33541 gttttttaga aagttaaaag ctagaatacc taaatacaaa aaaatagaac tagatgaata
    33601 ttccagtttt gtttttttac aaattgatgg tcaaaggact gttagagaaa taggcaagaa
    33661 cctagaagct aaatatggag aagaatctca tccactttat gaaaggttgt tagtattttt
    33721 aaatcatatt gaagttaatt gtcactatat tgaaagactg gatatataga tataataaga
    33781 attattatag atatattaaa gcatataaat tagaaggtaa ttatgaaaga ggctgtctca
    33841 aaatagagat aagcctcttt tgtattgttg aaatgatgaa aacatatttg atttctcata
    33901 aaaaagagtg tctcataaaa ctaaaaagtt cattttgaga cactaccttt aaaatattta
    33961 taaagtatag atttaaagca aaacttatta aacttaagat tattttaata tcacttatta
    34021 ttagctattt catcagctag attatttaaa tattcccatc tttcatattt gtattcaagt
    34081 tccttttcta gtttagattt ctcatctaaa atttcttgaa gtttagtaaa atcagttgaa
    34141 aattttgcag tactttcatc aagtgttgca attttagatt cgagtttttc tatatcagag
    34201 tcaatatttt caaactccct ttgttcatta tatgagaact taagcttttt atcattttta
    34261 ggtttttctt tttttagggt agtagagtca tcttttttaa ctgattccct tttagattct
    34321 tcaaattcaa ttccttgaat ctctctataa ataagataat cactgtaatt acctgtatag
    34381 atatgaattt ttccattacc ttcatatgca aatattttat tacaaattct atctaagaaa
    34441 tatctatcat gtgatactgt aactactaca cctttaaatt cgtctaggta ttcttctaat
    34501 attttaagcg tttcaatatc taaatcattt gtaggttcat ctaaaagaag cacattagga
    34561 gctgtcataa ggattttaag aagatgtagt cttcttcttt caccaccaga taatttacct
    34621 atcaaagtat attgcatagt accattaaaa aggaatcttt cacacatttg agaagctgta
    34681 atcctagtac catctgaagt ttctatatag tcactagctt cttttacata gtctatagcc
    34741 ctcatatcaa tattcatatg agagtcgtct tgagaaaaac aacctatttt aactgtttct
    34801 ccaattgaaa tatatccact atcaggctct atttctccat taagtatatt tataagggta
    34861 gatttaccca taccattttt gcctattata ccaattctat ctgtacgagt taaagtgtat
    34921 tctaaattat ctataacagt attttcttca aatgatttag atatattgtg tatttctatt
    34981 attttatttc caagtctact tgatgcaaca gatatatcga gattttcatc aggtttaaaa
    35041 gaatctctat tcataagttc atcaaatctt tgtaaacgag ctttttgttt agtgctacga
    35101 gcctgagctc ctcgttttac ccacgcaagc tctgttctta ttaaattttg tcttttatct
    35161 tcaatacttg cttctagcga aagtctttcc attttctttt ccaaaaatat tgaataattc
    35221 ccatcataac tgaataatct acccttgtcc agttcaataa ttctatttgt aacacggtct
    35281 aaaaagtatc tatcatgagt tatcataagc aatgagcctt ttcttgaatt aagatattcc
    35341 tcaagccaat ctatagtatc attgtctaga tggtttgtag gctcatctag tatcaaaaac
    35401 tcacaaggtg taataagggc tgatgccaga gagactctct ttctttgccc accagaaagt
    35461 tctgctattt tttgattaaa gtcatttata ccaagctttg taagtactga cttagcttca
    35521 ctttctaaat cccaaagatt taaggcatct attttttcgt gtactgataa taatttttta
    35581 tttaattcct ctgtaaagtt tgaattaagt ttttctagga tttcttgata ttcaccaagt
    35641 agctttaatt cttcagaagt tccattaaat acttgttcaa gtacagttga attttcattg
    35701 taagttggat tttgaggtag atattctatc ctaataccat tagtttttgt tatattacca
    35761 gtctcacttt cttcaactcc tgcaataatt ttgagtaagg tagatttacc agtaccattt
    35821 acaccaataa gtcctatttt ttccccctca tttattccaa gagatatatt ttctagtaat
    35881 tttttttcag aatagctttt acttatattt tccaatgtca ttaaattcat ataattcacc
    35941 tttatttcta atctattatt tttcaaaatg ttgataatct gggtttttcc aatagccacc
    36001 ccaactccaa cctctgctta caaaagcatt ataacaatca tcaccttttt ttatcattcc
    36061 aaattttgtg ctagacctat cagcataaac tgtacttatt ttaggtgtaa catcatttcc
    36121 agaaacttga ggattctgta atgggtttat atctatagca cgtcctttac catgatttga
    36181 aactacattt gtaccagcta tagttctata acaaaatgat gaggtattat tatcagacat
    36241 agatttttca tctacagcat catagtcatc tattaattta attttttcaa ttggatattt
    36301 tttttcgtat aattccttaa aaatatccac tacttctgga gctagttcac tgtttactat
    36361 catttctcct tgatgagttt tttcatcaaa tccataatat gtaagtttta aataagatag
    36421 actatcaaag cttataggtt catcagtagg catagattta ccaatcattt tattttttat
    36481 attatctggt attgaagaat atgaaaaagt aggcttgata ttattatctt cctgcttttt
    36541 ggtagagctt tgattatttt tagaatcatc tgttgtatta tttttattct gcatagtttc
    36601 ttgactcttt atattacctt ttttgttggg attagagaac agtatctcat atatagataa
    36661 taaagcaaga aataatacag ttattacaat cgttaatttt aatttattaa tttttcttcg
    36721 ttttgacttt ttactcatat tatcccccag tattagattc atataaacta tatatattat
    36781 acctcataaa aataaactag gctaacaata tttagttaac ctagaacatt tcatttatag
    36841 tttctccaat tatttttatt ccaatttcaa tgtcttcaaa agacaaccta gaaaatccga
    36901 gtcttaaagt atcaaaacca gagttatctg taaaaaataa atctccaggc ataaatataa
    36961 catttttttc ataacatttt tctaataaaa ctctagtatc tatatttttt agttttataa
    37021 atatatgcaa acctccatca cccaagatat attctgtttt tatatatttt tttatacatt
    37081 tatatgcaaa gttaaatttg tccccataga atttacgtat ttttttaatg tatttttcaa
    37141 aacctccatt acttaaataa tcaaataaaa ttccttggtc taaaaaggat acatgtatgt
    37201 ttctgcacct ttttacactt tctagtttat tgattacttt tttatctgca aatatccatc
    37261 caattctcat tccaggaaat aaaatttttg aaaaactacc aatatatacg accccattat
    37321 tcatattatc tagggaacag ataggaaata tatgagaact attgtagagt aattcttcat
    37381 taaatccatc ttctataatt gctatattgt attttttcat aagattatag aatttgtacc
    37441 tattttcagg agtcattaca attcctgtag gattgtgata agagggtgta atgtatgcaa
    37501 actttatttt atttttatac ttaactaaat tgtcttctag catattgaag tccaaaccat
    37561 cttcatttat atctacacct aagatattga ggttatgaga tttcataatt tttatagcag
    37621 tattatgagt aggattttca catattatgt agtctttttc ttgggtaaaa gatgacataa
    37681 taatatcaaa gccttctgtg aatccatttg ttattagtat atctttatta cttatatcag
    37741 cacccttgtt tgtcatatat tctagtaaat agtcaataag gggcttataa ccttgtgcat
    37801 atccatagtt gagtaattta tgtccttcta gtgaaattct atttaaaaag gattttttta
    37861 attcttccat gtcgaataaa tctccatctg gagaaatact tttaaaggaa atcaggtcag
    37921 agctccaagg aatttcactt tttataatgt ccaattcatt ggctttttta gagtatgtat
    37981 tctctaaaca atcccagttt aatgaccatg tagtatttga agacttattc tttgatttaa
    38041 caaatgtacc ttttcctgaa atagagtaga ttaatccttc tgattttaat tcttcataag
    38101 ttaaaactac agaatttcgg ctaacttgta aaagctgact aagctccctt gtagaaggga
    38161 gcttactatt gtctggaatt agtccttttg atatcatatc acttatataa tttttaagtt
    38221 gtatataaat tggttcatta tcatttagtt ttagatttga aaatatcaaa ttaccacctc
    38281 aaattatttc atctaattta aagagtattc tataagttta tctaatagtt cactgtactt
    38341 aatacctcta gcagcagcac ttctaggaat taaactagtt tgagtcattc caggaagagt
    38401 atttagttca agaacatatg gaataccttc acttataatc atatcaactc ttacatatgc
    38461 tttgcaatta aaaattttcc aacaagtctc agaaatttcg tttactcttt tttgaagttc
    38521 ttcatctaag taaacaactt cttcctttgc acctttttca actgagtatt tagcttcata
    38581 atcaaagaat cctgaatcag attttataga tattgtaggg aacacttctc catttaatat
    38641 gaatgaagta tattctccac ctggtatata tttttcaatc attacaaatt cgtcaacttc
    38701 cagacctttt ctaactgctt cttcaacttc atccttagaa tgaataaaga atgtggctac
    38761 acttgaacct cctgagtttg gttttataaa tactggataa cctatattat ctatttcatc
    38821 atagtcgatt tcatcaactg attttactaa aacccatgga gcagtaggta ggtttgaatc
    38881 tctaagcatt tttttagtta tatttttatc catacacatc ccactgcaaa gaggtccaca
    38941 tccagagtat ggtatatcca tagtctcaag tattgattga atacatccat cttcaccaaa
    39001 ttttccatgt aaagcaagta ttgcaaaatc tatatcttct ggtatctttg taaatatatc
    39061 ttttttatca tctattatta cttttacaac ttcatattta tttttatcaa ggttattata
    39121 tatctctttt cctgaattta aagaaacttc tctctctgat gatattccac ccattataac
    39181 tgctattttc atattataca ctcctatttt aatttttatt attttaaagt aaacttttta
    39241 tttacatact taattatatg aattaacagt tgaggatgaa agtaccacta tatacgaagt
    39301 ttatattatg tgtggtttta gaataataat ctagtttaaa gatatgttta aatgattttt
    39361 taactttatt tatgttatta tttttgctaa aattattgtt atgaacttaa gactagtctt
    39421 aatggctata aatcaaggag tgataaattt tgaaagctga aataataagt gtaggaacag
    39481 aaatattatt gggagatata gtaaatacaa attctcaatt tttagctaaa gaacttgcat
    39541 cattgggaat agaagtatac catcagagta ctgttggaga taacaaacaa agacttttag
    39601 aatgttttga tgaaagctta aagagaagtg attttgtaat tactacaggt ggtcttgggc
    39661 caactggtga tgatatgaca aaagaaactg ctgcagaata ctttggtcag aaattagaac
    39721 tacataaacc ttcactagag gttttggaat ctttctttgt taaaacagga aaaaaaatgg
    39781 ccgaaaataa tatgaaacaa gtgtactttc caaaagatgc tatagtacta aaaaataata
    39841 atggtacagc tccaggggca attttaaaaa aagatggtaa atttataata gtattgcctg
    39901 gacctccaag agaaatgaaa gctatgttta atgagagtgt caaaccttac ttacaacaat
    39961 tcacaaatga aatgctagta tctaagactt taagattata tggaataggt gagtcaaatc
    40021 tagaattaga aattctcgat ataattaatg agcagactaa tccaacagtt gctttatatg
    40081 caaaagaatt agaagttaca ataagaataa ctgcaaaggc tgaaaatgaa agagaagctt
    40141 ttaaattaat aaaaccagta gaagaaaaaa taaaaagtag agttggtaaa tatgtatata
    40201 ctgaaggaga tatttccata tctgaagggg agactgcttt agaagatgct gtttctaagt
    40261 tacttgtaga aaaaaatcta acaatagcag tggctgaatc ttgtactgga ggtttggttt
    40321 cttcatctct tataaactat ccaggaatat cttctgtatt tcttgaagga tgtgtaacat
    40381 attctaatga ttctaaaatg aaaagattag gagtaaaaag agaaactttg gaagagtttg
    40441 gagcagtaag tgagcaaaca gctatagaaa tggctgaggg cgttgctaaa ggacttaaag
    40501 ctaatatagg tatatccaca actggagtag ctggacctgg tggaggaacc aaagagaagc
    40561 ctgtgggact agtttatact gcaatatata taaatggaaa aacaatagta aagaaaaata
    40621 tatttaatgg ggatagaaga aagataagat taagagctac tagagattta cttaatgaat
    40681 tgagaataca attagaaaag ctttaagttg ttatgatatt ggatatattt gaagtgttac
    40741 tataatataa aattgttttt ggaggctttt aatttatgat aaaacatata ttttgtgatt
    40801 tagatggaac tctatatgaa aatggaacca taacaaaaga agatatagta gcaattgaag
    40861 aaatagaaaa aaagggagta cagtttaatg ttgctacagg tagaattttt aaacaagcac
    40921 ataatattat caaagatagt ttagatatga atggatatta tgtatgtgaa aatggttcat
    40981 ttatatatga taaagattat aatgtaattt ttaaaagaac gatagatgat aatttagtaa
    41041 aaaaagtcat tgatagattt gaatcaagtg atgcacaact ttattttaaa tataagggtg
    41101 atgtaattgt gggtagtgat accactgctt ttagacatta ttctagtgac tttatagttg
    41161 acccagattt tgaaaaaaga agtagttttg ataatcttat aggaaatata ggcgtatgtt
    41221 ctgaaaattt ggaagagtta tccagaatag aactttatct aaaaagtgag tttagtgaag
    41281 tattagaaat atatttttct ggaacatata ctttaaatat agttcctaaa agtgtgtcaa
    41341 aaagaggaag tatagagcat gtaataaaaa cgttaaatgt aagtccagat gaagttgcta
    41401 ctattgggga ttctccaaat gatatttgca tgttagaagg atttaaatat agttttgcaa
    41461 tgtctaaggc aagggaagat gtaaagcaaa gtgcaaatta tgtagtagat agtgtaaaaa
    41521 gtgccataga tgttataatg gagataaata gttagtataa aaaatgactg tcctagagaa
    41581 gtataaaatt atattctata gagcagtcat tttaattata ttcaattata agtttatata
    41641 aatttaaaaa ttcttaagtt aaccgcaatt tgctatatga tatgatttta ttgtattaga
    41701 taaaatagag tcaagtatat aatttatgac actattacaa gtataagctg aaaattttaa
    41761 atcattcata taatcagttt tctttttgta atcactataa agtttagtaa aaaacttctc
    41821 aaaactacta tttatagtac cacatttaat tttagataaa tcaacatctt ttgcaaatgc
    41881 tccaagttct ctttcataag taacatgttt atttaagcta tgcttaaatt cccaacgttc
    41941 actgtgagct acacagaaaa aatcacataa aaaatgacat atgactccta gttcctgact
    42001 aagtctactt actgaaaagt atttagaaag actatctata ttaagactgc atagatagtt
    42061 tattttagaa acaatcatat taaatgactc atctaaataa tgttttttta atttatattt
    42121 agaaaaagca tcaggcttta tattgccata tataaaattt ttttcgctta taaagaatgt
    42181 tttattttca tctatattat caagtattga ttttgccata ataaaatggg tattcattaa
    42241 catatttaca cctcgtatag tttattagac ttattaaata tattattatg atatgatatt
    42301 tatgataaaa gtttaatggt aaatatatga aactaagtca acagtattta tttaaataat
    42361 ttcaaccata ttcataataa cagtaaatgg ccaaatttac aatatgtaaa tagtgttaag
    42421 tttttattta tcaaatttaa aattaagtat tagtatactt tctaattaac ctattggctt
    42481 tagactgtga gatttcaagg tgtttggcta ctttataaga actaccaaat ttttcataag
    42541 catccaaaat tatatttttt tcatactctt taattaaggt gtcaaaattt ttatttttat
    42601 aattagtaga ttttgagtca gatataataa ccttattaat ttgggatata tgtgttttat
    42661 ttccattatt ggtaatatgt atattttttg taagaatgtt tggtaagtca tctattgtta
    42721 tatgattatc tgtacaagta agcaagattt tttgaattat attttgtaat tctcttatat
    42781 tgcctgggaa tctataattt gcaatagcat ccatagcttc tttagaaata gttttattta
    42841 ggttgaagtc acttgagtac ctattaaaat aatattttat aatttccact agattatccc
    42901 ttctttctct aagaggaggc aattttattt ctataacatt taatctatag tataaatctt
    42961 ctctaaactt tttttctttt acttttgaaa ctaaatcagc atttgtagca gatataattc
    43021 ttatatcaac ctttttattt tttaagctcc caactggagt aaaagttcta tcctgtatta
    43081 attgcaaaaa ttttgcctgt agattttgtg gtatatctcc tatttcatct aaaaaaagag
    43141 ttcctccatt agctaattct acgagcccaa ctttaccttt agtacttgca cctgtaaaag
    43201 cacctgaagt atatccaaaa agttcagatt caagaagttg tggagatata gttgcacaat
    43261 ttattgttat aaatgggcca ttagatctat tgctgttgtg gtgtatatat ctagcaagtg
    43321 agctttttcc tgttccagat tcacccaata aaagtacact tacatccgtt ttagaaacat
    43381 gatttactgt gtttatgatt ggtctcatca ctattccgtc cataattaca gtatcttcaa
    43441 ttctaagtgc agttctgtat agtgtttcta cctcagattt atattttttt acttgctttt
    43501 ttgtgtcttc aagtttattt tttatattat taagttcagt tatatctcta caattttcaa
    43561 cagtaaattc taagtttcca ttttcatcaa atataggggt agcagttatg ataagctttt
    43621 tccctgtttg agtatcttgt tccagtgaaa atttagtctt tgtttttaat acaattggta
    43681 tggggctttt tgttgaatat ccagagtctt ctaaaaaaga aacatttttt cctaaaatat
    43741 cttctttttt tagaccataa ttttcttcaa atgctttatt acaatatatt attattcctt
    43801 tatcatcaga aacacatatt tcatcatggg aagcttctaa tattttttga taaaattcca
    43861 aatctaaatt catttttata ttttcctcct aaatttttaa tgatatatta aaataggttt
    43921 aattgattaa tatacttaaa
//
LOCUS       DAMKPG010000026        42537 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMKPG010000026 DAMKPG010000000
VERSION     DAMKPG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.028"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            146..496
                     /locus_tag="PC621_001486"
     CDS             146..496
                     /locus_tag="PC621_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF4108648.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            515..2158
                     /locus_tag="PC621_001487"
     CDS             515..2158
                     /locus_tag="PC621_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF4108649.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2239..4296
                     /gene="recG"
                     /locus_tag="PC621_001488"
     CDS             2239..4296
                     /gene="recG"
                     /locus_tag="PC621_001488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF4108650.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4355..4909
                     /gene="rsmD"
                     /locus_tag="PC621_001489"
     CDS             4355..4909
                     /gene="rsmD"
                     /locus_tag="PC621_001489"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF4108651.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4911..5408
                     /gene="coaD"
                     /locus_tag="PC621_001490"
     CDS             4911..5408
                     /gene="coaD"
                     /locus_tag="PC621_001490"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF4108652.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5412..5936
                     /locus_tag="PC621_001491"
     CDS             5412..5936
                     /locus_tag="PC621_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108653.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6280..8199
                     /locus_tag="PC621_001492"
     CDS             6280..8199
                     /locus_tag="PC621_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4108654.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8212..8499
                     /locus_tag="PC621_001493"
     CDS             8212..8499
                     /locus_tag="PC621_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108655.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8632..9891
                     /locus_tag="PC621_001494"
     CDS             8632..9891
                     /locus_tag="PC621_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF4108656.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            9894..10634
                     /locus_tag="PC621_001495"
     CDS             9894..10634
                     /locus_tag="PC621_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF4108657.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10957..12267
                     /locus_tag="PC621_001496"
     CDS             10957..12267
                     /locus_tag="PC621_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF4108658.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12365..13282
                     /locus_tag="PC621_001497"
     CDS             12365..13282
                     /locus_tag="PC621_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF4108659.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13298..14107
                     /locus_tag="PC621_001498"
     CDS             13298..14107
                     /locus_tag="PC621_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF4108660.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14164..15912
                     /locus_tag="PC621_001499"
     CDS             14164..15912
                     /locus_tag="PC621_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF4108661.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16175..17197
                     /locus_tag="PC621_001500"
     CDS             16175..17197
                     /locus_tag="PC621_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF4108662.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17202..19508
                     /locus_tag="PC621_001501"
     CDS             17202..19508
                     /locus_tag="PC621_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF4108663.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            19480..20595
                     /locus_tag="PC621_001502"
     CDS             19480..20595
                     /locus_tag="PC621_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108664.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20613..22031
                     /locus_tag="PC621_001503"
     CDS             20613..22031
                     /locus_tag="PC621_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF4108665.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22046..23254
                     /locus_tag="PC621_001504"
     CDS             22046..23254
                     /locus_tag="PC621_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108666.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(23335..24723)
                     /locus_tag="PC621_001505"
     CDS             complement(23335..24723)
                     /locus_tag="PC621_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF4108667.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
     gene            25065..26399
                     /locus_tag="PC621_001506"
     CDS             25065..26399
                     /locus_tag="PC621_001506"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF4108668.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26528..27445)
                     /locus_tag="PC621_001507"
     CDS             complement(26528..27445)
                     /locus_tag="PC621_001507"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF4108669.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27653..28537
                     /locus_tag="PC621_001508"
     CDS             27653..28537
                     /locus_tag="PC621_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF4108670.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            28750..30591
                     /locus_tag="PC621_001509"
     CDS             28750..30591
                     /locus_tag="PC621_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDF4108671.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            30895..31602
                     /locus_tag="PC621_001510"
     CDS             30895..31602
                     /locus_tag="PC621_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108672.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            31596..32906
                     /locus_tag="PC621_001511"
     CDS             31596..32906
                     /locus_tag="PC621_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4108673.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            33023..33706
                     /locus_tag="PC621_001512"
     CDS             33023..33706
                     /locus_tag="PC621_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108674.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            33699..36086
                     /locus_tag="PC621_001513"
     CDS             33699..36086
                     /locus_tag="PC621_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4108675.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            36365..37507
                     /locus_tag="PC621_001514"
     CDS             36365..37507
                     /locus_tag="PC621_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF4108676.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            37526..38050
                     /locus_tag="PC621_001515"
     CDS             37526..38050
                     /locus_tag="PC621_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF4108677.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            38150..38968
                     /locus_tag="PC621_001516"
     CDS             38150..38968
                     /locus_tag="PC621_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4108678.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            39046..39357
                     /locus_tag="PC621_001517"
     CDS             39046..39357
                     /locus_tag="PC621_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF4108679.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(39660..41663)
                     /locus_tag="PC621_001518"
     CDS             complement(39660..41663)
                     /locus_tag="PC621_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF4108680.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(41816..42454)
                     /gene="recX"
                     /locus_tag="PC621_001519"
     CDS             complement(41816..42454)
                     /gene="recX"
                     /locus_tag="PC621_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF4108681.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        16646 a         4561 c         7361 g        13969 t
ORIGIN      
        1 aattttatcc aaaaactttt ctaggtttaa attataccat atttatagta tgatatgatt
       61 ataattttat ataataaata agtaaaaata gtaaaaaata aattaagaaa tgtttttaat
      121 aaaaataagc ttaagggggt caaccatgag tgcaaaggta acgaatcaat atggaaatat
      181 agaaatagat aatcaagtaa tagctcaagt cgtatataga gctgcaatgg aaagttatgg
      241 cttagtaggt ttagcatata agtctaaagg aatagtagag ctattaaaag gcgagaacgc
      301 aactaaaggt gtaagagttg aggaagtaga agaagatgcc atagcaatcg aactttatgt
      361 tataatacaa tatggtacaa atatatctac tgttgccaat aatataatag atagagtaaa
      421 gtatgtagtt gaaaaaatga ctggcgtaag agttactaga attgatatta atgtacaagg
      481 aataagagtt aagtaatttc ataggaggaa acaaatgatt caatatatag atggtaaaag
      541 gttaagggag atgtttattt caggtgcaaa caatctccaa aacaataaag agttagttga
      601 caagttaaat gtattccctg taccagatgg agatacagga actaatatgt cattaacaat
      661 atcttatgcc ttaaaagaat tagcaaaagt tgaaaatgat aatatatcag atatcggaaa
      721 agcattatct aaaggttctt taatgggagc tagaggaaac tcaggagtta tactttcaca
      781 aataatcaga ggtatagcta aatctataga aggaaaaagt aaattatcaa cagaagattt
      841 agcaaaagct tttaaaaatg gttcagacac agcatataaa gctgtaataa aaccaattga
      901 aggaactata cttactgtag ttagagaaag tggagagttt gcaataaaaa ctgcgaaaaa
      961 agagaaagat gtagtaaagt ttttaagtat gcttgtgaag gaatcaaatt cttcattaga
     1021 aagaactcca gaccttctaa agaatctaaa agaagctggt gtagttgact ctggtggaaa
     1081 gggtcttgta cttatatatg aaggaatgtt agcttctata aaaggcgata acatagaaat
     1141 aaaaaatgtt gacctagata ctaatatttc aacaagtatg gattttgcaa agagtactac
     1201 tagcacagat gatataaaat attgctattg tacagaattt attttagaaa gttctaaggt
     1261 agaagatact aaaataagag acataatgat ggcttatggt gatagtctag cagtagttgg
     1321 agatgatgga gtcataaagg tacatgtaca cactaatgac ccaggaaatg tacttcaaga
     1381 agctctaaag tacggtcaat tattaactat aaaaatagaa aatatgaagt tacaacatga
     1441 aaatacatta ctggatgtag aagaaaagaa agaaaatgat agtgaacctt tagaagaaga
     1501 gaaagagttt gggtttatag ctacttctat gggtgaaggt ttggcaaata tatttaaaga
     1561 tttcggagtt gaccatataa tagaaggtgg tcaaactatg aatccaagta ctgaagattt
     1621 tatgaatgct ataaagcata taaatgctaa aaatatattt attttcccaa ataacagcaa
     1681 tataataatg gctgctaatc aggcaaaaga attgagtgat aaaaatataa tagttatacc
     1741 tacaaaaaat acgcctcaag gatttgctgc attagtaact tttaatggag aattaagcga
     1801 agatgaaaat aaagaagcta tgatgaatgc tttaaactca gttaaatcag gtcaagtaac
     1861 ttttgctgtt agggatacag tgatgaatga aattgatgtt aaagaaggta atataattgg
     1921 aattgcagaa ggaaatttat tatcagcagg agattatgtg gatgaagtaa catcaaacct
     1981 tattgaaaag ttggttgatg aagatactgc tataataaca ttattctttg gtgaagatgt
     2041 tacagaatct caagcaaatg aactacgtac ttctttagaa gaaaagtttg aagatgtaga
     2101 tgtagagtta tattatggag gacaacctct ttattattac ttaatttcag ttgaataatg
     2161 attataagaa cctgtatgac caagtagctt ttataaaatt ggttgtacag gatttttgtg
     2221 tagtaggtga tatatttgtt ggatttatat aaagatgttc aatatgttaa gggaatagga
     2281 cctaaaaaag cagataaatt aaataaactg ggtatattta cactaaaaga cctattgtat
     2341 tattttccaa gacaatttga agatagaaat aatctaaaga aaatagcaca attagaagat
     2401 ggtgagaaag ttacgataaa agcagtaata tcaagtataa atacatttag cccaaaagaa
     2461 ggtatgactt taacaaagat tgatgtaaaa gatgagacag gctcggctaa attagtgttt
     2521 tttaataaaa gttatataaa aaatactttt cgtccaggag attcaatatt agtttttggg
     2581 aaggttaaga aaaaatttaa taacttagaa cttacatcat gtgaattaga atatcttact
     2641 aattcaccta agaatacatg tagatttatg cctgtgtacc agcttacata cggtgttaca
     2701 aataaagaaa ttatgagtat aattagaaca gtacttgaag ataaagaatt aattatacaa
     2761 gaatatatgc cacaaagaat tatagaaaaa tataggctat gtagtataga ttttgcagtt
     2821 agaaacatac attcaccaag tagtaaggaa tcattaaaaa tagctttata tagaattgta
     2881 tttgaagaat tacttatact acaactaggg ttgtttgtat ttaaaagtgg cagaaataaa
     2941 gaagatggaa taaagtttga aacaagtaaa gacttaaaaa aaataataag tgctttacca
     3001 tttaaactga ctaaagctca aaacagggca ttagatgaaa ttatacaaga tatgaattta
     3061 gagaaaataa tgaatagatt ggtacaaggt gatgtaggga gtggtaaaac agtagtagct
     3121 ctcttagcac ttgcaaattg tgttttaaat ggttatcagg gcgctttgat ggcacctaca
     3181 gagatattag cagggcaaca ttacatttca cttactgagt ctctaaaaga ttttggaata
     3241 aatgtaggtc tattaatagg tagtcttaca aaaaaacaaa aggatacagt attggaacaa
     3301 ataaggaata atgagattga tatattaata ggtacacatg cactaattga agataaggtt
     3361 gaatttaata atattggttt agttataaca gatgaacagc atagatttgg tgttatgcaa
     3421 aggagcaaac tttcattgaa aggagcaaat cctgatatat tagtaatgac agcaacacca
     3481 ataccaagaa ctttagcact tatactctat ggagatttag atatttctat tatagatgaa
     3541 cttcctccag gaagacagcc aatagaaaca attgctattg aaaaaagtaa gcgagataga
     3601 gcatataata accttgtaag aagagaggtt gaatcaggaa gacaagtgta cattgtatgt
     3661 cctttggtag aagaaagtga agctatagaa gctaaatcag cagttgaact tgtagaggaa
     3721 ttaagagctg aatatttcca cgatttaagg cttggattgc ttcatggtaa aatgaaatcg
     3781 agtgaaaaag acgaagtaat ggaacgtttt aaaaataaag aaatagatat tttagtttca
     3841 acaacagtta tagaagttgg tgtcaatgtc ccaaatgcaa cattaatgat aatagaaaat
     3901 gcagaaagat ttggacttgc acaattacat cagcttagag ggcgagttgg aagaggtagt
     3961 cataaatcgt attgtgtact aatttatgat tctaaaacag atgtatgtag acaaagaatg
     4021 gctataatgg aagagaccaa tgatggtttt aagatttcag agaaggattt agaaataaga
     4081 ggacctggag aattttttgg gacaaggcaa catggtcttc cagaacttaa agtagcaaac
     4141 ttatttaaac atataaaaat attgaagtta gcacaacaag aagctcgata tatattgggt
     4201 gaagataata atctacaatt gaaagaaaac atggcactca aaaaagaaat tatagataaa
     4261 tttaaagata cattgaaaga aatttcatta aattaatttt aaattatgtt aaaatattac
     4321 taatgtaata atgtgctgaa gggagattgt catattgaga gtaatttcag gaaaagcgag
     4381 aggattaaaa ctaaatactc caaaaaatga agatgtaagg ccaactacag atagagtaaa
     4441 agagtctcta tttaatatga taaattcata tataatggaa agtgaagttc tggatttatt
     4501 tgctgggact ggttctttag gaatagagtg cttatctagg ggtgccaagt catgtacttt
     4561 tgtagatatc agtaaggaaa gcatagatat tgttaagtcc aatgtaaaaa aggcaagagt
     4621 tgaaagtgaa agtattattt taaaccttga ttttaagact gcaatagata aacttaaatt
     4681 acaaaatagt aaatttgaca taatttttat ggaccctccg tattataaaa atatgtttat
     4741 agaagctatt gaaaaaattg ataattctaa tctattgaac gaagatggaa taatagttgt
     4801 agaacatgat acgaatgatt tatttccaga taagatatgt aaattggaga aaactagaga
     4861 taagaaatat ggaaatacta cattaacttt ttataagatg gaggcataat atggaaaata
     4921 aacctagaaa agctatattt gcaggaagtt ttgacccaat tactaatgga catttagata
     4981 ttatttgcag ggcttcgaag ttatttgacg aattacaaat aggagtatta aataatccta
     5041 ataaaaaggg tctatttagc tttgatgaaa gagtaaaact tatagaaaaa agtacaagcc
     5101 atttaaataa tataaaagtt gtgacttttg atggtttatt aataaattac tgtcaggaaa
     5161 atgggattgg agcattagtt agaggtgtga gaagtggtgc tgatgttgat tatgaacttc
     5221 agatggcaca tatgaataga gagcttaatc cagatataga gactataatt ttaccaagtt
     5281 gtacaaaata ttcatttata agttcatctt taataaaaga ggttttgctc tttgatgctg
     5341 atataaaaaa tttggttcca aagattgttt tggaagaatt aaagaaaaaa actagtgggg
     5401 gtaattgata tatgaagata gatttagaaa tgattgaact atttgagcag ttagaggaga
     5461 tatttagaag tgcttctaca atccctttct cacataaatg cacagtggat aaagatgagg
     5521 ttttagcaat aattaatgat ataagaactt taataccaga agaagtaaca caggcagtat
     5581 ggattaataa agaaagaaat aaaataataa gtcaagctaa gcaagatgct acaaatatag
     5641 ttgaacaagc gaaaaaagaa gctgagagaa tacaacaaga atatcaagat aatatagagg
     5701 aattaaagaa aaattctgaa gatgtagtaa aggcatatgt agaatccagc gaacctgttg
     5761 ttcaagcaga ccaaagagca aaagatatag ttgatagagc tgagagaata gccaatgaga
     5821 taagaattgg gtcaattgaa tatgcagagg atgtgctttc tagtgttgag tataatttaa
     5881 gagaaatatt agaagaagta aaaagagata aagctgaact tagacctaaa aaataaattt
     5941 taaataaata aaaatggagc tatcacacaa aaaattccaa ttgatgtgtg atagctctct
     6001 tttaatatag caattttttg ttatataatt gtgcttttta gattacttat atcttatttt
     6061 gatattataa tgtagtttaa tcttttattt gttgaaatca acttatttat ttgtataaat
     6121 aatataggtt attttcaaat ttttttaata attttaccac tgtcttcttt tagattaggg
     6181 ttagactgtg caaaattata aaataatact aaatcatgtg caattatttg gtatggcact
     6241 acatgtagaa aatatagata ataaaattaa ggaggtagta tgaaggaaag ggctaaaaaa
     6301 atattagatt ttattattaa aaataacaat gtaacatcac aagaactaca agaaaggttt
     6361 aatgtttcaa agagaactat ttattacgat attctagcta taaatgaaca actaggaaaa
     6421 agtggtaata taaaaaatgt aaaacacaaa tttatatttg aaggtaattt atgtgatgca
     6481 aggaaaataa tttcaactga agaagataaa tttttagata gtgattacag aaaaactttt
     6541 atattaaaca agatattact tggcgaaaaa atatcaatag aaaaactaac aaatgaaatg
     6601 ttactttcaa aaaacacagt agtacaaaca ataactgatg taaaaaaata ccttcaaaca
     6661 atggggttaa gattagagta taagggaaag tacaaaatta taggtgatga atacgttatt
     6721 agagagctgt ttctgataat tgttcaagaa aatgtactgg aaattaattc tataagtaag
     6781 gaagtaagta gtttcgatac aaaaggacat ataaaactta ctgattattc tttattaaat
     6841 ctaactaagt ttgtggaatt tctaaataaa aggataagag atgggaaaac attatactca
     6901 tataaatatc taaatgaagc taaaaaaatt agttacttta gtaattgtaa agaactttta
     6961 tgtgaagaag ccaatgaaaa tgaacaagcc tatatatgca cttacataag ttcacttcct
     7021 agcttaaata gtgaagttaa ggaagatgtt gttgaagaat atgtagataa acttatagat
     7081 aagtttgaag taaatacagc aataaaatta gaaagtaagc atgagtttaa gaaaaatatt
     7141 ttaaggcacc tccactcttc ttacaacaga atacgattta agtttcctat tagaaatcca
     7201 atgttagatg agactaagta taaacatgag tcactttata aaattataaa atcaattata
     7261 gaaaatgaag aagagtttcc tgtatttgag gggataagag aagaagagat aggatttata
     7321 gcagcgtatt ttggagggta tttaagaggg tctagggata atggcctaag aagaaataaa
     7381 gtcttactag tttgtcctaa tggtcttatg gtatcaaaga gtttggaaat acaactatat
     7441 aaatacatac caaccataga aatagtaggt atagtgtcca ttaagcaact aaaagaagta
     7501 aatgtatact acgattacat aattacaact atagatatac aaaatgtaaa caatgtaatt
     7561 gttgtaaatc ctcttttaac atcatcagat gttcaattac ttatgaacaa actaataagt
     7621 gtaaaagaaa acgagaagta tttcaatctt gaattaataa tacaagctat aagaaaaaat
     7681 ggagttatca ataatgaaga agcactaaaa gctgatttat taaacataat acacaagata
     7741 gatgaaggag agatgtatca accaatgtta aaggaattaa ttaatgcaga aagggtcaat
     7801 ataataaaaa gtgttagaga ttggaaagaa gcaataaaaa tagcctcaaa gccattgtta
     7861 gaagataact ctatagaaga gttatatata gaaaatatga taaagtcagt tgaaaaatat
     7921 ggaccatata ttgttttagc tgatagattt gctctaccac atgcaagttc taaagaagga
     7981 gtaaacaagc ttgctatgtc tttactaata gtagaagatg aagttgattt attaggaaaa
     8041 ccagtaaata tatttatggt attagctgct gtagataaca ctactcacat tagagcatta
     8101 gcatcactat cagagatgat gtacgaggaa gaaaatgtaa aactaataat taatggtgat
     8161 aaaagttcaa tactagaact tataaataag caaaattagg aggaatgaaa aatgaagata
     8221 ttagcagtat gtggttttgg tgtaggttca tcaatggtat taaaaatgac aatacaaaag
     8281 gctctaaagc agttaggtat agaatgtgaa gtagaaaata cagatataaa ttcagctaga
     8341 ggtgctgatt gtgactgtat attcacaagt caagaattgt taaatgaatt aaaaggaact
     8401 tgtaatgtag atgtatatcc agttaaaaag tacatggatt tagcagaagt cacagaagtt
     8461 gttcaaactt ttttaagtga taaaaatgat aagaaatagt ataagtttta aaaagtaaat
     8521 ttaagttaat cagaataaaa taaaaactta ctaaattcaa aaatagtaag aaagagagga
     8581 gggatttagt ggtagaaaac ttaaacagtt gacctatcac taaaaaatat aatgcttgat
     8641 tttatttcaa ataatatttt acgtaatcca tctatgttaa ttggattaat agcagcattt
     8701 ggtcttgtac tacagaaaaa gaaagcatct gatgttataa aggggtcact acttgcagct
     8761 ttcggaatta taattatgga gactggaaca ggtatgttag ttagttcaat agcaccaatt
     8821 aatggagctt tccaaagtat aagtggtggt gcagtaacaa aaggtttaag tgatgttact
     8881 tttactgcaa gttatggtgg aattataggt ttagcaatgt ttgcgggttt agctattcac
     8941 ctaatgatag caagatttac tcctattaag acaatcttct taacaggtca catgctttat
     9001 tggttcccat tcatatttgt agcttcagga gttgaagctg gattaaatgg tggagtatta
     9061 atagcttttg cagcagtatt atcagcactc tattggtcaa taatgccttg gttattaaga
     9121 aaatatgtgt ttgatgttat aggagatgat tcttttacat taggtcatcc atctggttgt
     9181 ttagcactta tatcaggatt tgttgctaaa ttagtaggaa ataaagaaaa atctacagaa
     9241 gatataaaca tacctcaaag tttatcattt tttagagaaa tatctataag tggtggaatg
     9301 gcagtgtttt taatgtttat agttgttggg atagtaatac caggtgcgtt taaagatagt
     9361 aatgagttta ttggaacgta tgcaataaag caaggtcttt cttttggagc aggtcttata
     9421 ataatgcttc atggggtaag aatgctaata aatcaaataa tgccagcatt tcaaggtata
     9481 tctgaaaaag ttgtgccaaa tgcactacct gcttttgact gtccaatatt atttaattat
     9541 aaaccaaatg ctgtattaat aggattctta gttgctatgc taacatctac agctatgatt
     9601 ttaatatgta actactttaa tgtatttaat ttgctactta taccacttgt tataacttca
     9661 ttctttgagt gtggagcagc agcagtaata ggagaatcac aaggtggtat acgtggagct
     9721 ataataggta cagctatagc tgcaatattt atggtagttt tagtaggaat atctgctgta
     9781 atttactcag gaactattca aaattggata ctaatatttg gaggtaacga cctttcatta
     9841 tttggtataa ttagtaagtt tataggttca ctatttggag gaatattata gatatgaaat
     9901 ttaaatacat agatgagata agaagattaa ttgatataat agaaaacgaa gaaaaagtca
     9961 atatggaaaa agcagtacaa atccttgttg aagcaataga aagtaaaaat gctatatttt
    10021 catttggagc ttcacatgca ggaatattaa cagaagaatt attctataga gcaggtggct
    10081 gtgttcttat aaatccagta tttgcaaaat ctctaatgtt agatacagcg cctataactc
    10141 acacaagtaa gatggaaaga ttagaaggat atggtacagc tctagctgat aaaactccaa
    10201 ttaaaaaagg agatgtagtt atagttcatt cagtatctgg tagaaatcca gtaagtatag
    10261 agtttgctat gaaagctaag gaaaataagg caatagtaat atgtataact aatttaagtt
    10321 actcaaaaag tgtatcttca agacattctt caggaaaaaa actttatgaa gtatgcgatt
    10381 tagtgataga taatcatggt caaaagggag atgcctgtgt agagatagat ggattaaacc
    10441 aaaaagttag tccaacttct actgtaatag caactactgt gatgaactct attatagctc
    10501 aagctactca ggaattagtt aataaagggc taaagaatcc tccgattttc tatagtgcca
    10561 atattgatgg tggagatgag ttaaataaga agatttttga tgaatataaa agtgtaattc
    10621 attatgaata ttaaggttaa aaaaatatgt taatgaactt tgaagtttat caacaaagaa
    10681 tattaataat atataataaa tgctatcatt taaaagctgt aggtaaaaat ctacagcttt
    10741 ttattattgt agattaaaaa gatgcgtaat taaattttaa attggagaaa ataaattgga
    10801 aggtatcaat tataaatatt agaaacgttg tcatagaaaa aaactatatt aaaaaaaagt
    10861 acatagaaaa aactgattag acaataaata catgctttga taatatttta aatggagtga
    10921 aaatgtgtag aattataatg aaaaaagaaa ataaaaatga aaaaagaaaa taaaatggag
    10981 ggaataaaag tgggtatgtt aaaaaaatta tgttcactag ctatggtagc aattatgact
    11041 acaacactta taacagggtg ttcaagtgga aatggaaaag attcaaaaaa ggatggcgaa
    11101 aaagaaaaac tagttctttg gatgccacca gtagaaaata atatgaagga agtttgggac
    11161 cctattttag ataaatttga ggaaaaaaat aactgtgaag tagatttaca gataattcct
    11221 tgggataatt attctgaaaa atttgcaaca gctataagtg ctggcgaagg tccagatgta
    11281 ggatatatgt atgcagaaat gtatccacag tttatagaaa gtggagcagt agaagatttg
    11341 actaactatg caactaaaga ggataaagag caatctattt atataaaaac atctgaaatg
    11401 atgggtggaa tgtatggtat gccattccaa gcagcaaatc caggggtatt atactataac
    11461 aaggatattt tagagaaatt aggagagaaa cctcctaaga catgggaaga ttttaaaaga
    11521 atttgtaaaa aagcaacaca agatactgat ggtgatggaa agatagacca atggggatta
    11581 gctcagggat ggggagctaa aacttttggt aacttaaact ggaactggta tccttattta
    11641 tggcaagctg gtggagatat ttttaatgat gatttaaaat cggttaaatt taatgataag
    11701 agtggtttag aagcagctaa tttcttaaaa gaattacaag catatgtgcc tgaagattct
    11761 ttatctaaag atagtaatga aatgatagaa agcgtatttg gaccagggaa agcagcattt
    11821 acaataatgt tatcttctgc tgcaacaagt gtatttgatg aatcattccc ggatttaaat
    11881 tgggggtttg taactggact agaagataag aaagcagcaa cttttggagc agtagaccat
    11941 ttatctttaa tgtcatctgc taaaaataaa gaacttgcat acaagttaat acaacatatg
    12001 ttaagtgttg aatcaatgac agaatttcat aaagcaatac caagagcacc aataacaaaa
    12061 ggtgaaccat atcaaggtga tgaaagattc aaagaaatgg tggaaaatga caaaaatgta
    12121 tatagaccat tagtagtagg acctcatgga gttgaaatat atgaatattt atggaaagaa
    12181 cttcaaacta tgatttcagg agataaaact ccaaaacaag cattagatga tgctgcaaaa
    12241 tattcaaatg atttattagc acaataaaag tttaaagctg taatatgttg taaaggactt
    12301 tacagattaa aattagcatt gtatttataa atacaatgct aatatttata ttggagagga
    12361 taaaatggaa aacttagtta gagagtttga agaaaattca aaaagaaaaa aaacaaaatt
    12421 aaatattaaa ccatatatgt atatattgcc attgggaata attttagtgg cgttttatgt
    12481 gataccaata ataatgtcaa tatactttag ttttacaaag tataatatta ttagtccagc
    12541 cacttttata ggtttagaaa attataaaaa attatttaca gatgaaacat taaaagtatc
    12601 tattataaat actattaaat ttactgttgt tgtagtacct tgccaaacta tattatcatt
    12661 gattttggca gtttggatta caggaaaagg aaatagtaaa atagcttcat ttgctaaagg
    12721 tgctatattt ataccagtat tatcatctat ggttttgata ggtatggtat ggagggcttt
    12781 attgaatgga gaaggttcta ttatatatca ggtattaggt gcctttggaa tagaatcatc
    12841 taaactttta ggagatagta agacagcatt acctacactt atgtttatat ctatgtggaa
    12901 aaatataggg tatttcatgg taatttatat ttcagctata atgaatttac caaaacactg
    12961 ctatgaagta gcgaaggttg atggggctac aaaacttcaa gaatttataa aaataactgt
    13021 acctttattg aaaccaacta caataatggt tgtattttta gggtcgatat ggtcattaca
    13081 agtatttgac ttaatttata ctgtgacagg tggaggtcca ggtatatcta ctatgagtat
    13141 agttatgcat gcatttaact taaactttaa aaactttaat tctggatatg caatgactgt
    13201 agcaaacgta ttatttttgt taatagcagt agtttcgatt ttacaaaata agcttgtaaa
    13261 acgagacaat tcagattttt agaaaggtag gtataatatg aaaacagcaa gaagattatt
    13321 aagtgatggt ttacttttat ttatagcgtg tgcaagtttg gtacctttta tatacatgtt
    13381 gataatatca ttgaagataa catataactc atatagtttg gatatatctt tttcaacagt
    13441 aactcttcaa aactatatag atatctttac taaaaaagga tttgcacaat atttttttaa
    13501 tacagcaata gtatcttttt caggtgtatt attaaattta gtatttagta cattagcagg
    13561 atattctttt gcgaagatgg attttaaagg tagtgataag ttatttttat ttatgataat
    13621 gactttaatt ataccttccc aagttacaat gataccacta tatataataa tgaaacactt
    13681 aggatggata aatagttatc ttgcacttat tatgccaata ccaactgcat ttggcgtatt
    13741 tataatgaga caagctattt taggagtacc aaaagagtta ttagaatcag caaagataga
    13801 tggttgctcg gattttagaa ttttaataca aatagtattg cctttaatca aaccagcatt
    13861 aattacacta gcaatattta cttttatggg agcatggaac gagtttatgt ggcctcttat
    13921 agctacaact aaagatgcaa tgagaacatt aacagttgga ttatctactc ttaagacatt
    13981 ccagataact aattatggac agatgatggc aggggctaca ataacatttt tacctccatt
    14041 tattttctat ttgattttac aaagtaaatt tgttgaaggt gtgtctttgt ctggtataaa
    14101 aggttaaata gtctaattta ataacataaa agaaacttat ataaatatgg aggaatttaa
    14161 actatggaaa tttcatatgt actgaaagat tgcacattca aacacaataa atactcagtt
    14221 atagaagctg aaacttggaa agagaaggat ttggatattg tagtagctaa aggtgaaaaa
    14281 tttgcatttc aaattatgct aaaagcaaca gaagaattta attgtacaat tgataaaagc
    14341 agtactatat catggaaagg actggaaaat agagtaagat tagcattaaa tgtgcctaat
    14401 agtttagaaa ataattttag tataaatata ttaggatatg ttcaagatga tacaaaggca
    14461 tttgtgaatg atgctatttt aagggataaa gatgtgttag tagagcaata tttgcctcaa
    14521 acattttgga ttgaaggtca agtaccagaa gattttgaag aaaataacct taatataggt
    14581 attgatatat ttaaaagctt tggatatgaa gatgaagaaa aagtttgtac aattgatgtt
    14641 cctgtaaaag taagaaatgt cgtgctaaag cctttagata aaagcaagtt ctttttagat
    14701 ttatggcaac atccaagttg tttagctaga atgtataaag ttgagttatg gtctgatata
    14761 cattttgaaa tagtcgataa ctatttaaaa gaattagcat ctttaggaga aaaagttgca
    14821 acagttatag tttcagatta tccatgggca gggcaaagtt gttataaagt ttataaaaat
    14881 ccatcaaacc tatatgaata taacatggta agtgtatcaa aaggattgga tggaaagatt
    14941 aaatgtaact ttgagagtat ggataggtat ataagcatag ctgataagta taaaatgtct
    15001 aaggagatag atttatttgg tctattaggt aattggtgtg ctggtgagtt tggaaatcct
    15061 gtagaaggtt ataaggaccc tataagagta agatattttg atgaaaatga taaagtattt
    15121 aaatttataa acaatacaaa tgatttaaaa gaatatatag gattagtatt aaatcattta
    15181 atagagtgtg gattatggga tagagtaagg attatagctg atgaaccaaa taatccagaa
    15241 gtagtcaaag agtgtataga atttataaat tctactgtag gtactcatca agttaaatat
    15301 aaatcagcaa ctcacgacca aaacttttta gatagagcta aagatgaaat tgatgatatg
    15361 tcaataaatt taaaacttac tatacaaaat tataaagata tagaaaatct taagaaaaaa
    15421 atcaatgaca agggtgggat attgacttgg tttgtatgct gttttccaga gaaaccaaat
    15481 agttttttaa gttctccttt tgtagaaaat agaataatag gatggtatac ttattacttt
    15541 ggtcttgatg gatttttaag atgggactat aatttatgga cagaagaccc ttggaaagat
    15601 tcaagttaca aattccctat ttggaaagct ggagatatgt tctttgttta tcctggaaaa
    15661 gatttaaagc cagttagaag tgtcagaatg gagaatttaa gatttggaat acaggacttt
    15721 gagttattta caatgttaga aaaagaaaag ggcagagagt atatagaagt tgagcttatg
    15781 caagaattat taaataaaaa agaaaatgct gaaattaaag gttttggtga catagaatta
    15841 ggatattcac ttgataattg gggatatgat aaagttaaaa aatatgtatt agagttgtta
    15901 gacagagtat aaaaaaatat aagagtgtct caaaatgaac tttttagttc atgaggcact
    15961 ctttttgtat gaaatcaata tagtttcatt gtttcaacaa tgaaaaatag gattacttat
    16021 attctaagat agccctttag ttttttagtt gtattcaatg ttttaaaact aactataaac
    16081 gctataaagt ttataaatga caataattat atgtaacatg atgtacttta atttgctata
    16141 atagggattg aataatatta tatggaagat aacattgttt ggggggatta atataaaaag
    16201 aataggtatt attataatgt tttttttaag tatagctttt attggatttt atcttgcaat
    16261 aagaggtaac agtgatacag ttaagatgca acgagggata aatataggta atgcactaga
    16321 atcacctaag gattttccgt gggatgtaaa aatgtcaaat aagttttttg atgatataaa
    16381 agatgcaggt tttgacacag tacgtatacc agtaaggttt tctgattata cgtcagattc
    16441 tgataatttt aaaattgatg aagaattttt taaaaagatt gataaatatg tagattatgc
    16501 attggataaa gacttaatag ttgtgttgga tttacatcat tttgaagaaa ttatgaaaga
    16561 accaagagtt cataaagaag agtttttaaa aatatggcaa cagatagcta ataggtatca
    16621 aaaatatgat aaaaaattgg tatttgaatt attaaatgaa cctaaagaaa atttatcttc
    16681 acaattacta aacgaataca ttgaagaagc aataaagata attagaaaaa ctaaccctaa
    16741 aagaactatt atagtgggac catataattt ctatcaaatt gattatttga atgaattaaa
    16801 tgttccaaaa gattctaata tagtagtttc ttttcactat tacgaaccaa atgattttgc
    16861 atttcaagga aacatttatc ataatggatt tgagcacttg agcaatatta cttgggaagg
    16921 aacaaatgaa caaatggatt atttaaaaaa aaggtttgat attgtagaaa attgggcaaa
    16981 taaaaataag gtaaaaatat tcttgggtga atttggagta acaaaagaag caccagaagc
    17041 ttcaagaaga gcttggataa aagctgtaag agaagaagct gaaaaacgaa acttttcatg
    17101 ggcttattgg gagcttgctt ctggatttgg tatatacaat caaattgaag ggacttggga
    17161 tagagacata ttaagtgcat taatagaaaa gaggtgattt tatgaagaaa aattcaaaaa
    17221 agatacttat aactttaact attattacta atatcgtata tatattgtgg agaatttttt
    17281 acacagtacc taaagaagaa gggatgtttg ccttaatctg tgctataata cttctttttg
    17341 tagagataat gggtatgatg gaaatgtttg tacattatta tggtatgtca aatattgagt
    17401 atcctgaaaa accagtcata agtgaagaac tttatccaca tgtagatgta ttcattgcaa
    17461 catataatga gtctgttgat ttggtgagaa aaactgtaaa tggatgtatt catatgcagt
    17521 atccagataa gaaaaaggta catatataca tatgtgatga tggaaatcgt gaagaaatgc
    17581 gtattctagc agaaaaaatg ggtgtaaatt acataactag aactgaaaga gaaggagcaa
    17641 aagctgggaa tttaaataat gcaatgcaac atacaaactc tcctttgatt gctacgtttg
    17701 atgcagatat gataccaatg catgactttt taattgctac agtaccatac tttttgaaaa
    17761 atgagcaagc taaaaaagat ggagaaaaag aggagtatga aaaggttggt tttgtgcaaa
    17821 ctccacagag tttttataat ccagacttgt tccaatttaa tctgcactct gagggaagga
    17881 ttccaaatga gcaagattat ttttacagag atattcaatt ggctagaaat aggacaaatt
    17941 ctgtaattta tggtggtagt aatacagtta tttcaaggga agcacttgaa gaagttgatg
    18001 gattttatac ttattctatt acagaagatt ttgcaacagg tatcttaatt caaagtaagg
    18061 ggtatagatg ttatgctatt ccagaagtac atgcatcagg tttatctcct acagatttaa
    18121 aaagtcttat taagcaacgt gaaagatggg cacgtggatg tatacaaact ggtagacgtc
    18181 taaatatttt gtttaggaga ggtttaggtt tttggcagaa aataagttat atttcttcaa
    18241 ttacttattg gtatgcaagt ataaaacgct ttgtatatat aatggcacct attttatttt
    18301 ctgtgtttaa tgtgattgta gtaaaatgta cattacttca agtattggta ttttggttgc
    18361 caatgtatat tttaagtagt ttatctctta aaatattttc tcaaaatata agaaatacta
    18421 gatggaccaa tatatatgaa actataatgt ttcagtcttt aatgccagca gtaatacttg
    18481 aaacgtttgc aatatctaaa aataaatttt cagtaacaaa taagaataaa ttagaagaaa
    18541 atagaatgta taagttttta cagggaatac cttattttat ttacatggtc ttatctatca
    18601 taggaatttt aaaaatgttt gtagcaatat ttaaaatgag ttcaatgaca tattcagttg
    18661 tattattttg gttgattgga aaccttttta accttgttat ggctacatta tttatatcag
    18721 ggagacaaca gttaagaaag tctgagcgtt atattgctga aattgatttt aagttaaagc
    18781 aaaactctta tgttctttct tcaaaaacga ttgatatatc agaaaatggg tttgcatttt
    18841 tgctagaaaa tccagaatat atttccccag aagaagagtt cgaggtagag tttagagaaa
    18901 aatcaggaaa tgaaatgtat atagctaata tgaaagcaaa gattgttaat gttgtggaag
    18961 taaattcaaa gtggaaatat gcagcttata ttacacatat tgaagatagt gaaatagata
    19021 attggatgtg tattgttcac gatagaatac ctactttacc gatgacaata tctaatcaac
    19081 taggtttctt tgatgatttg caaattaatg taaaaaaacg tattgaaaag attagaacat
    19141 tatcacgaag aagtccaaga atcaatatga attttcagat ggatataaaa aatacaggta
    19201 aattaagaat agtaaatttt aattatcaat atgtattatt aaattttgag aataaaaata
    19261 tttatcctaa agaaatagca ttggaaatta atgaaaatat tgtgttagaa tgtgatttat
    19321 gtgagggtaa aatcgatgaa agaggtctct tatataaagt aaataacatt gacagcataa
    19381 tgcaaaattt ctttttaaga gatgaaatga tggattggat attacaaaat aagacaattc
    19441 ctgtttcaaa tcctagtgaa aagaaggaaa aaagtataga tgagtttgaa cccatggaat
    19501 atatttagga gagatgaaat gaagacttta aaagtgtttt tttgttctgc aaaatatctt
    19561 attttggtgt ttattttttt tataaatact acttttataa gttcagcgac aagtgataaa
    19621 attttagatt tatcctttaa aaagatagag actgatttaa gttctaggat tacatatgaa
    19681 gatacaggtg taaaaataga aacagatagt tctaaaagtg ataaggaacg ttatttatat
    19741 atttaccaaa atataaaaga aaattggagt atgtataata atttttatat tgaaattcaa
    19801 aataaaaata aaagttccca gaaaataaat ttaagtatac aaagtaagaa tatgcttgaa
    19861 tttaggttaa aagaaggtag tgaaatattt ttggaagata aaaatataat atattcagat
    19921 aaaataaaag aaggatgcat agaagtacca ggtgaatttg aaggcaaaat ttatgtgaac
    19981 tttaatagcc ttataaatga gaaaagtaat gttgtgctgg actcaagtat gctttcaaac
    20041 attgttagtt ggggaattac atttattcca tctgatgaag aacataatat tgttattata
    20101 aaaaagatat ctttattgtc tgaagagaaa ttaagttttt tacataatat taaaataact
    20161 ggcaatgaag aagtacaaat accagtattg ggtcaaagta taagtcagta tgaggtatta
    20221 ggtcttaaac gtgattctaa aattaagtat tctcttatgg gaaaacaaga taatgtgagc
    20281 atatctcaaa atggtaaatt aacattaagt aataaagccg aacctggaca aattatttta
    20341 caagttaatg ttgatgataa gtttaaaatt ggaaggaaaa tagcattaac tgaatcttgg
    20401 agtattaata aaaaagataa ggatggtgtt ccatatacat tggtatctcc tgagcaatca
    20461 ccaacagtac aatatatgaa aaaaataaac tttatgaata acatcataac gtttgttcga
    20521 attttatttg tatctctagt aataatatgc tttggaatat atttatattg gaaaaaatgt
    20581 tcaaaaacta agtaaagaga ggaggttaga ttgtgttatt ttcaagttta atttttttat
    20641 tttatttttt acctataact ttggttttat attatgtatt taggtttaac cgaacgatac
    20701 agaacatgat attgcttgct gttagtttat ttttttatgc ttggggagag ccaaaatttg
    20761 ttgttatcat gatagtttcc ataataatga actatatatt tggtttacta gtagatagat
    20821 atagggaaag taaaataaaa gtaaaagttt ttttagtttt gatgtgtgta tataatatag
    20881 gtgttttatt tatatttaaa tatctagctt ttgcgctaag aaatattagt gaacttatta
    20941 atactgaact aactatacct agtattgttt tacctatagg aatttcattt tttacatttc
    21001 agggaatgtc ttatgttata gatgtatatc gtagacatgg agaagtccaa aagaatcctt
    21061 tttatgtggg actgtatata gcatttttcc cacaattaat agcaggtcct atagttagat
    21121 atgagagtgt ggcagaacaa attctcaata gaaaagaaac ttggaataaa ttttctattg
    21181 ggacttgcag gtttataaca ggtctaggca aaaaagtatt aatttcaaat aacatggcta
    21241 tagtagcaga ctatatttac actatgaata gtcaaggaga aatagctgca tcacttgcat
    21301 ggcttggttc aatagcctat actatgcaaa tattctttga tttttctgct tattcagata
    21361 tggctatagg tttaggtctt atgtttggtt ttaaatttga agagaacttt aattatccat
    21421 atatatcaaa atcaattagt gagttttgga gaagatggca catttctttg ggaatgtggt
    21481 ttaagagcta tatatacttt ccgcttggag gttctagagt agcaaataaa gatattatga
    21541 ttagaaatat gtttatagtg tggttattta ctggaatatg gcatggagca gaatggacat
    21601 ttgtaatttg ggggatttta aactttgttt tcttgattat agagcgattt ataatgtttg
    21661 aaaagattga aaatcacaat tttataaaac atatttatgc gttattagta gttaactttg
    21721 ggtgggtatt gtttaggtca cctaatttaa aagaagcata taattatttc aaagctatgc
    21781 ttggaggcaa tggagttata tggagtgatt atacatatat gtttttaaaa gaatactggg
    21841 ttttctttat atttgcattt atttttagtg tacctattgc aaagaaaatc aataagtttg
    21901 ttgttgaaca agctaaatat agtatgatat ttaacgtatt ttatccaatc tcagctattt
    21961 tattgttctt tatttctgtg acatatttag taactggaag ctataatcca tttatttatt
    22021 ttaattttta gttgggagga aaagtatgaa tagatttttt aaagtaagat taataactgc
    22081 aattatgttc ttaattattt tattcgcttt ctctatcttt aatatttgtt attctggaaa
    22141 agatatgata caggagataa aaaattcagt tgctgaaaaa agtagtttaa aagatacaat
    22201 atcatctatt gataacacag taaatgacaa agtaatatat aaaaatacgt tcattgaaac
    22261 ttatggctac ttacaaaagt taatgggtaa aaatgagttt tcaaaattta gtgttgtaaa
    22321 agatacttct ggaaagatgc attatacata ttttgctaaa gctccaaatc cagttggtgt
    22381 gatagcagat agggtaagaa aattttctga ggaaatggaa aagcaaaaca ctaaattagt
    22441 ttacttaatg actccagata agtatataaa aggagtgaca aaatttccaa aaggaatacc
    22501 ttattcttat agcaatgaga cagcagataa ttttttaaat caactaaaaa agtataatgt
    22561 ggactatatt gattttagag aaaatatttt aaagagtggt attccaaaag atgatttatt
    22621 ttttaaaaca gaccatcact ggaaagtaga aacttctttt tgggcatttg gagaattagt
    22681 tgagcaactt aataaaaaat acaatatgaa tttagatgaa aatcattact atagagataa
    22741 agaaaattat aactcaattg tatatcctaa gtcatttctt ggttctatgg gaagaaagac
    22801 tggtatctta tatggtggta tagatgattt tacattaatt tatcctaaat ttaagactaa
    22861 ttatacttat tatacagatt ctaaatctca aaaatttgaa ttaaaaggaa ggtttgaaga
    22921 gagtttaata ttatcatatc cttttaatgc agaccttgat ttaatggatg gtcaatcaga
    22981 taagtatttt acatatttat tggggaatag accattagtt aaaataaaaa atatagaaaa
    23041 tccagatgga ttaaaagtgc tgtttgttaa agattctcta attgtaccaa cagcagcatt
    23101 tttttcctct gtttgttcat ctatagatat gatagaccct agatattatg atggagatat
    23161 actagaatat gcaaaatccc atgactatga ctttgttttt atatctgtat atccccaaaa
    23221 tcttacaaaa gaattttttc cgttttgtaa gtaaatttct aaagaaattt tcttataaaa
    23281 taaaaaataa atggctaact agttaaaagt tagccattta tcttaagtgt tactctaaga
    23341 aatgtttttt ttaccaaata cttctttatc tatttttcca agtgattttc ctacagtaat
    23401 tgatgatacc atagcaccat taacatttat catggtacgt cccatatcta gtatagggtc
    23461 tattgcaagt ataccaccaa ctaatggaaa ataagaacca agaccaactc cagagataac
    23521 aactgaaacg gccattgtag cagttcctgg aagaccagct attccaaacg aactaattgt
    23581 tataacaatt aacaacataa cataaaggct catatccatg tttactccag ccatattggc
    23641 aagtgttaca gccattaaag caggatatat acctgcacaa ccattcatac ccatatttgc
    23701 acctaaactt gctgtaaagc ttgatacacc ctcatcaact ccgtgatttt gtactagact
    23761 ttctatcgtt acaggcaatg ttcctaaact agaacgtgaa gtaaatgcca gaagtaaagc
    23821 ttcagttgaa tttcttatat aagtaatggg atttagacca tttaaagtta taaaaattaa
    23881 atgtatggta aataatattg ccactgatat ataaagagct aatataaact gtaaaacaga
    23941 tactattgag ctaatacctt gtgaagttat tgtatttgca agcagagcaa ccacagcata
    24001 aggcataaat ttaataacag tcatagacac acttacaatt attttgtaag atgcttctat
    24061 aaaatctata aatggtttta ttgtttcaaa gtgtttttta cttaatctct ttattgaggt
    24121 agctataaat gtagcaaata ttattgtagc tacaatattt ccatttgcca tagccattac
    24181 aggattagat ggtagtaatc ctcttaaagt atccacaaga gagtttattt ctttaagttc
    24241 ttgattttca actaaagtag gcatattagc tcctacacct aacttcatta tatttgcaac
    24301 gataattcca acaattgcag ctatagcagt agtagcaaga agcataccta aagatttaaa
    24361 tgtcagagtt ccaagattgt tatctttcat atttataatt acttttataa tggataagaa
    24421 aactaaaggt actactaaca tttttaataa gtccataaat ccagaaccaa ataggctata
    24481 ccacttgctt acttcttgta gccaagtgac ttgagtggta tcattaggaa aaccagcaaa
    24541 agcttgtatt atgattccca atatgagtcc tagtactgta gcatatattg ttctcaatga
    24601 aaattttatt tttttctttt caaattgtct aacaataaag aatgtaccta gaagtaatat
    24661 tatgaaaaaa atcgttttaa aatctgtaat cataagaaat tgtgataaaa atgaactttc
    24721 caatattttt tctcctttta tttaaaaata aatttttcta agtttaaatg ataccaatat
    24781 attaaaaaaa tgtcaatatt atatcacaat ttacttaaat agagtaaaat aaattgattt
    24841 tgaaaaatgt gattaagtat agaaaaatag ggtataataa atgtttaaag aatttgaatt
    24901 gtggtaagaa aataaaaaat agcttttata atatttgatt tcaaattaaa atatgttaaa
    24961 taaataacta aaaattgtta tgaaaaatgt ttacatagaa tatatatcaa atgaaaagct
    25021 taaaataagt gaagaaatta actagacatc aaaggagaga taaaatgaga gtagtaataa
    25081 ttggtggtgt tgcagctggt atgagtgctg cagcaaaatt aaaaagaata aaacctgagt
    25141 atgaagttgt tgtttatgaa aaaactgaaa tagtatcttt tggtgcttgt ggattaccat
    25201 actttgttgg tggttttttt gatgatgctg atgaattatt agcaagaaca ccagagaaac
    25261 ttagagaagc tggaattgat ttaaatatat ttagagaagt cgttgaggtt gatagtgaat
    25321 ctaagaaaat aaaagtaaaa aatataaaaa cagatgaaat atatgaagat tactatgata
    25381 aattgatgat agcaactgga gcaagaagta ttatgcctcc tataaaaaac ataaaattga
    25441 agaatgtttc tacattaaaa agtttatatg atggagaata tctaaaaaaa cttttaagta
    25501 atgaagataa taaaagagtg acaataatag gagctggttt tatagggtta gaagcagttg
    25561 aggcatgtaa gaaattaggt aaagatgtag atgtaattca gttagaagat agaatattgc
    25621 cacaagtatt tgataaggag atgacggatg tattagaaga agaaataaat agacataatg
    25681 ttaatttgca cttggatgaa atggttgtag aactttgtgg agaagataaa gtagaaaaag
    25741 ttataacaaa caaaggggaa atagatacag atgtagtaat aattgctact ggtgtgagac
    25801 ctaataccga gtttttgtca aatactaata taaaaatgct aaaaaatgga gctatagtag
    25861 ttgatgaata tggaagaact tctgtagaag atatatactc tgctggagat tgtgcaacta
    25921 taaagaatat agtaagtaat gaaaatgtat atgttccttt agctactgga gctaataaat
    25981 taggtaggat agtaggagaa aacctagcag gaagagaagt atcttatcaa ggttcactat
    26041 cttcaagttg tataaaaatt atggatatgg aggcagcatc aactggaatt acagaaaagc
    26101 aagctaaaga tttaggcata aatgtcaaat ctaaatttat ttctgattat aatcaaactc
    26161 attattatcc aggaagaaat aaaatatatg taaaacttat ttatgatgca gatacaaaag
    26221 ttattttagg cggacaagtg gctgggttta aggatgctgt ccaaagagca aatgtattgg
    26281 cagctgctat ctttggaaaa atgaccacaa gtcaacttgg aatgttagat ttatgttatg
    26341 ccccaccatt tgcaagaaca tgggatgttt taaatgtagc tggaaatgtg tctaaataat
    26401 atttagatta tagctaatat agttgacaag caattaaggg aatgaattta cctaaactat
    26461 ttttatcatg gtaaaattca tccctaaatt tttattccaa atttttagaa ataaggattc
    26521 cattaaatta ttcatcaaaa tttaaacgca agcttaatct atcttctcca agaatagtat
    26581 tttcaaaaac attagtagca ttttctaaat acatactagg tatgtctaaa gaaggactaa
    26641 aatgggtcag taaaagttgt ttgacatttc caagttttgc aagattagca gcttctctaa
    26701 aagtcatatg tttgtttcta acagcttttg atatatctaa atcatcacca tacatggctt
    26761 cacatataaa taaatcacta tcttttatga aatctggtat tgattctata ggtcttgtat
    26821 cggttgtaag acttatctta actccctttc tcaattctcc taagaccata tctggagtgt
    26881 attgttttga atttaaaact atattttgac cttcttggag tttgttccat aatattttag
    26941 gaacttcgtt tttagttgcc ttatctatat caaatttagg gtttcttcta aaattaaaac
    27001 tgtatcctaa acactctata gagtgttcta aactgattgt tgaaatttct aaatttctta
    27061 aaactttatt atctaaagaa aaattgcctt gagggttttc tataattttt aaagtgtatg
    27121 gtacatattc tactaaattt ctcatagaac gtatacaatc gactattcca actggaccaa
    27181 ctatagttaa atcacttgta cgaccactat tcccaattgt tgataaaagt cctaaaagtc
    27241 caaatatatg gtcaccatgt aaatgggtta tacaaatcaa gtctatatct ttaaagccac
    27301 atttttttag cttcatagat acctgagtac cttcaccaca gtcaattagt atttttctac
    27361 ctttatagtt tataaataca gaggatagaa atctgtttgg cataggtaca tttccaccac
    27421 atccaagtaa agttaaatct atcatttttt cccctttcta aaattaacac aaatctgttg
    27481 tttgttttta ttatgatatc ctattataat tattttaaca ttaaatataa caataaatgt
    27541 gtatttgagt caagaaatat tatcaaataa agtttttata tagcaatgta taaaaatttg
    27601 gagaacgaaa agtgaaaatt atatttagga gtatttaaag gaggaaaatt taatgaaagt
    27661 attaaagaaa attactattt tgtcattgat tacattaacc ttagttggtt gtagtaacgg
    27721 agaagataaa aaagtggttg aaggttctaa gaaaattcaa aattctaaaa aagatgaaca
    27781 gcctataata gtacataaag atgcaaatat attgaaagat aggtatgagc caccaaaagg
    27841 atttaaaaga gttgatgtag aaaaaggtag ttttgaatca tttcttagga atactaagct
    27901 aaaaaattat ggagagaaag taaagtatta tgatggaaga attaaggaat caaaaggtat
    27961 ttatgatagt gtatttgatg ttgatattgg caatagagat ttgcatcaat gtgcagatgc
    28021 gattatgctt atgagagcag aatacttgta tcagaataaa ctatatgatg aaataagttt
    28081 tgattttgtt gatggtttta aggcgaagta ttcaaaatgg gcacaaggat atagaatttc
    28141 agtaaaagat agtggttcaa gctggtataa agcaactgaa tattcaacat cttatgagag
    28201 tttcagaaag tttatggata tagtgtttgc atattcagga acgttatctc ttgaaaaaga
    28261 attggagcct gtaaaagttg aggatatgaa aataggagat gtgtttatag ttggtggaag
    28321 tccaggacat gcaagtataa ttatggatat ggcagaaaat gaaaagactg gtaaaaaggt
    28381 atttatgata gcccaaagct atatgcctgc acaacaaacg caacttctta ttaatcgtaa
    28441 aaatccaaat ttgagtcctt ggtatagttt agattttgaa ggtgatttga caatacctca
    28501 atggactttt aaaagagacc agttgaaaag attttaaaat aataatttta tataataaaa
    28561 gaaaaatata gatgtgaaaa aatgtaatta gattaaaact aattggatat ggacaaaatt
    28621 aaattgatag ataaatatta aaaattacac taatcaatct tactatagtt taatatttat
    28681 ctaaaaagtt ttatgtaaaa tataaatttt atattaaagc taaaactaat ttgaggaggc
    28741 taatttatat tgaagattag aaggtattta attttggtaa ttataattct aattacaaca
    28801 agtcagagtt ttatttctta tgggctagag cgatttgaag aaataaccat ttttcacact
    28861 aatgatatac atggaagata tgctgaaggt gatgaccata tacaaatagg aaatctagcc
    28921 acattgaaaa aagaaactcc aaactcaatt ttagtagatg caggagattg tttgcatgga
    28981 cttccaatag taaatatgga tagagggaaa agtgcaattg aattaattaa atgtgctgga
    29041 tatgactata ttactcctgg aaatcatgac tttaattatg gaaaagatag actgtttgag
    29101 ttatctaaaa gtgctaattt aggagagaat actttaaaat ttttatcatc aaatattttt
    29161 gaaaatggaa aaaaagtttt tgatagtaat gatataaaag aaatagatgg aataaaaata
    29221 ggattttttg gactctcaac ccaggaaaca aagaacaaaa ctggatttaa aaatgtggaa
    29281 ggattagagt ttaaaaatcc tatagaaagt gcaacagaac aagtaaatga attaaaagga
    29341 aaaggtgcca atgtaatagt tgcaatatgt catattggta caaatagttt aagtaagcca
    29401 aatagtatag atatagcaaa tgaggttcaa ggaattgatt tgattattga tggacacagc
    29461 cacactaagt ttgaaaatgg taaacaagtg ggtgatacat tgattgtaag tgcaggacaa
    29521 tatttagaaa atattggcga gataaaacta actttggata ctaatgatgt agaaaatata
    29581 aaaatagaaa ataaatcagt tagattaatt ggaaaagaag aagcattaaa gtataagctt
    29641 gactacgttg taagtaaaaa ggttgatgaa ataaaagaac aacaagaaaa aatattagat
    29701 aaagttatag gtgcaactac aaatactcta gatggttcat atgaaaatgt aaggacaaaa
    29761 gaaactaatt tgggtaattt aataagtgac attttactag ataaaactaa ggctgatatt
    29821 tctttattta atggagggaa tattcgagat acgatagaaa aaggcaatat aacaagaaga
    29881 gatattgtag atgtatttcc ttttagcaat actatagtta ccaaagaact tacaggagca
    29941 cagattaaag atgtattgga gcatggagta aaactttatc cagaaaaaag ttcagccttt
    30001 ttgcaagtgg gaggaatttc atactacttt aatcctaaac aaaaagaagg cgaaagaata
    30061 acaagtattc aaaaagaagg aaagccatta gatttaaata aaaagtatgt agttgcaacg
    30121 aatgattata tagcctcagg tggagatgaa tttccttgtt tttcaaaaga gccaatacta
    30181 aaggaatttg gaaatttaga gagtgcagtc attgaatata tagagtacaa aaaagaaata
    30241 tcaaaaaatg tagatggaag aattggtatt aaatctaaag atgagttaat aagcaagaat
    30301 catgagagtg atttgatatt aagtgaaaat gaaaaaaata tcaatgataa tgaagagaat
    30361 ctatctaata atataaaaag ctctgatagt gacaaaaata aaagcttaga taaaacaagt
    30421 gctagagaaa gttctaatca aggaatatct aaaagagaag ctaaagaaaa atctcctaaa
    30481 acaggagatt taggtttttc aaatagtata attatattta tagtttcaag tacattgata
    30541 tgtttattga attttaatca aaaagagtta aaggataaaa agtcaaaata aaaattaatg
    30601 agcaaaatta gtagatagta atttaatttt agataatagt aaacagcaaa ggaaattagt
    30661 tatataaata ctaatttcct ttgttttatg ctttaacatg attattttta aataaaattg
    30721 tattttatga aaaataatta cactaaatgg catagtatta tggggatttt tagtttttat
    30781 tcaagtaaat gaaatcatat taacaacttc tgatatattt acaaattgat aaaaaaatga
    30841 ttttattagt atgatataat ttagctagta tatatgatga tggaggatat agggatgaat
    30901 aatatattac ttttggaaga tgacaaatct ttaaatagag gaattagttt taaattaaaa
    30961 aaagaaggat acaatgtttt tagtgcattt ggtatagaag aagcaaaaaa tattttcgca
    31021 aaagaagaga tatgcttaat tatttccgat ataggtctac ctgatggtag tggttttgac
    31081 ttttgtgagg agattagaaa gaaaagtgat gtttacataa taatgttgac agctcttgat
    31141 gaagaggtgg atattgtcac aggatatgat ttaggtgcag atgattatat cacaaagcct
    31201 tttagcttga tggtattaat atcaaaagtg aatgcgctta tgaaaagagt aaatacagta
    31261 aaaaattata ctctattagt ttgtgatgat ttattctttt attatataga aaataaatta
    31321 attataagaa cagatgataa ggaagaagag ataattttaa gtaagacaga gacaaaacta
    31381 ttaaaatatt tgatggaaaa ttctatgcag acattgacta aagaacaact gttagaatct
    31441 ttatgggata gtagtggtaa ttttgtagat gataatacga ttgcagtaaa tataagaaga
    31501 cttagacaaa aggttgaaaa aaatccatct gaacctaaat atataaaaac agtaagaggg
    31561 gttggatata tgtggggaga aaggagtata aaaaaatgct aaaaaatatg aacctcaaag
    31621 atggtttaag tgtgaaagat gattatataa tgagaagagt tattgtatat tctgttttat
    31681 ctgtactaat agtaaccctt gtatttatac tttttgaata tagcagatta cataaattgt
    31741 atgaacatca aataaaaaat actcaaaata ttgctggaat attgattgaa aaatatccta
    31801 atgatgaaat agatatagta aattctattt ataattccga ttatagtaag gtgtctactg
    31861 gagaaaaggc atttaagaaa tttggctatg gattggaaaa taaaatgtca aatgataaaa
    31921 attttagttt atacttaaaa tcttttttta aggaatcatt ttttattttt ttaatgatgt
    31981 ttatagtagt tataggtata atatattatt ttataagata cataaataaa agattgagta
    32041 aaatttattt tatagtagat aagatggcac aagaaaacta cttgaaggac actactgagt
    32101 cagaagaaac tggttatcta gtagaatata atgagtattt taaagaaggt acatttagta
    32161 agataaataa ctgtctatat gagcttaata gaagtttaaa gataaagttt attaaattag
    32221 aaaaagaaaa agaaagtgta aaatcattag taacagatat ttctcatcaa ttaaaaactc
    32281 ctttagcatc cttaaaatta tacaatacat tacttataga agaaaaatta gaagaagatg
    32341 aagccaacga gttcttatta accaataagg cttctataaa taaattagaa aatttaataa
    32401 attctttagt gaacatatca aggttagaaa ttagcatgat aaacataaaa aaagaagata
    32461 atgatataaa aaatactatt ctaaatgcag taaaaagtgt tacccctaag gctaagagca
    32521 aaagtataac attaaattta aatgaattta acagtagaat tataccacat gataaaaagt
    32581 ggactgagga gagtattttt aatgtattag ataatgctgt aaagtacaca caggtaaatg
    32641 gagaaataaa tatatctgta gaagaaactg ctaatttctt taaaattata atagaggata
    32701 atggcatagg gattgaaaaa aatgagttta ataatatatt taaacgtttt tatagaggtt
    32761 ctgctgatga aattgaatct gtagaaggta gtggggtagg attgtattta agtagaaaga
    32821 ttttagaaga acaaggtgga aatattatag tttcttcaaa agtagggata gggagtaagt
    32881 tttctttgtt tcttacaact atgtaatatt aaatgaaagg aaaatgtaag cctctacatt
    32941 tattatataa gtataaacag ctccagaaat aaaaaattaa taaaatatgg ggtaatataa
    33001 cttagaatga ggggataatt ttatgaaaat actatatact gaaaatttat caaagcatta
    33061 tggaaaagga gaaaacttag taagagcttt ggacaatgtt aacttagaaa taaatgaagg
    33121 tgaatttgtt gcaataatag gaaaatcagg ttctggaaaa agtacacttt tacatatgat
    33181 tggaggtctt gatataccta catctgggaa ggtgtatata gataataaaa atatatttac
    33241 attaaaagaa gaagaactag ctgtgtttag aagaagaaaa atagggttta tatttcaaag
    33301 ctataattta attccatctc ttaatgtatg ggaaaatgta gtgttgccaa tcggtctaga
    33361 tggtagagaa gtagatgaat cttttataaa agaacttttg aaatcattag gacttgaaaa
    33421 taaacatgac gttttaccaa atacattatc aggtggtcaa caacaaaggg tggcaatagc
    33481 aagagcatta gctacaagac cagctataat acttgctgat gaaccaactg gaaacctaga
    33541 ttctaaaaca tctgatgagg ttatgagtat attaaaatct atgtctaaga agtacagtca
    33601 aactctagtt atgataactc atgatgacag tatagcccaa atggctgata gagttatatt
    33661 catagaagat ggcagggtgt caaaggttgg tgataaaaat gattaatact actaaaatag
    33721 caaaaagtaa tttaaaacaa aataaatcaa aaagtattct aataataatc actataatac
    33781 tttctacttt aatgctttct tctattggaa tttatattgt tgatgcagga gcatatcaaa
    33841 aagaaaatac tataaagtat tcaggtaatt atcaaggtat attagcaaat gtagatgaaa
    33901 aacaggctga tatattgagt aatcatgcag atgttgaatt aactggtgaa atgaatggag
    33961 taggtgttga aaaactggaa gatgattcaa atatttctct agcatacatg aatgaagatg
    34021 ctttgaaatt aaatagtttt gaacttatca aaggaaaatt gccaacaaaa gagaatgaaa
    34081 ttgtgttaga tagtggagct ttaaaggcac ttggttataa aaataaagac ttaggtgaaa
    34141 agataaaaat atcttataat gactataaaa atgataaaaa aattgaaaaa gagttcgtta
    34201 taagtggaat cttaaaaact agcgaaatat ctgaagcagg taaatattat tatgctatta
    34261 tatcagaatt atatatgaga aatactagag atatgtcaca agaagatttt aatatttatg
    34321 taaaatttaa tgataaaagt aatttatcaa tagagcaagc aaaagaaaaa ttagataaaa
    34381 ttgctaatga tataggactt gatactatta atactgctgt aaatgaaaat tatataaatg
    34441 ctttaaaacc agatatggaa acgattatgg gtggaggttt tgtaggatta gtaattgtat
    34501 tatctagtat tttagtaata tacaatatat tttatatttc aatagttaca aaagttcaag
    34561 aatttggaaa acttagagca attggtgcaa ctaagaaaca aataaaaaat atagtgttta
    34621 aggaagggtt tatattagcc ggaatagcaa taccaatagg aataatttta ggctatgtac
    34681 ttgcaaatat tataataaaa tcatttatgg atatagatgt aaaatcatca cgattaccag
    34741 ttatattatc ggttgtggta ataagcttta tatctgttgt tttatcactt ttaaaaccaa
    34801 tgaaagtagc atcaaaagta tccattgttg acgcagtaag atatacagga aataaaataa
    34861 gtaataagag taagagaaaa ggatataaaa atataaatct aaagagatta tcacatgcca
    34921 atttagaaag aaataaaaaa cgtacttaca tgacattggc atcacttata cttagtggaa
    34981 ctatatttat aacagtatca actgctttag aaagttttga tgctgagaaa atggcaagag
    35041 aacactttcc ttatgatata gaagttagac tatctggtta tgaaatgaac agtgataaaa
    35101 atcctaaaaa taatttgaat attttacaaa tggataatcc tctaagtaaa gattttttta
    35161 atcaaataaa gaacatggaa ggaattaaaa gaatagaatc tgcaagaagt gtaaaaattg
    35221 gtatggagga ctatgatgtt gaatttaagt atgacctttt acaaagtata aatgaaaatg
    35281 atgtaaaatc attaaacaag aatctaatag atggaaaaat aaatcttgaa agactgcaaa
    35341 ctggagatga aatagtaatt actcatgttg atacagcaaa agaaatggga gtaaaagcag
    35401 gagataaaat aagattaact ttatatgatg gagataaaaa gataaaaaaa gaatttaaag
    35461 ttcaagcaat agctatgggg gttccaagtt ttggtatagg gaaggatttt attgatagaa
    35521 ctttaaaata cgattcaact agtagcttgg gaatatatac agaagaagga aagtatcaag
    35581 aaatcaaaga tagtatcaag aagattgcta aaaataatgg atttttagag actgatttta
    35641 tagatagtag aattgagtca aataaggctg ttatttcatt tataaagata atgggataca
    35701 cgctaacagg cataataggt gtgattggat ttatgaatct tgtcaacaca atgataacta
    35761 gcatagttac tagaaaaaaa gaacttggta tgcttcaagc tattggtctt actaataaac
    35821 aattagtgaa aatgcttaat tcagaggcaa tgtattacac ttctggaatg atgataggaa
    35881 gtattttatt tggaggtata ttagggtata ttgctgttat atttctaaaa aagactggat
    35941 tatcttatgc aacatattct ttgccaatag taccaatctt gttgatgatt gtgtgtattc
    36001 ttatagctca atttataaca acttatttga taggacgaag ttttaataaa gaatctctta
    36061 ttgatagagt aagatacagt gaataaaatg caaatatttg tggttagttt aactagcaaa
    36121 aagcatataa aaaatatagt taaagagatt cataattaaa taggttaaag catagtacag
    36181 agcaaacaat ttaaacggat gtttgctctg tattttatta tgtaatcaat aatatatcta
    36241 atatactgat attattcatg ttagaaaatg tatcggtaca attctaaaaa agtataaaat
    36301 tgtaccatat aaaatgaaaa ttttgtataa taatcgtata gaaaataaat aggagctgat
    36361 atatatgaat aagaagatta attatgcacc aggaccaact gaaactaggg aaaatgtaag
    36421 attagcaaga gcagaaaaaa caacaaatcc agatatagat attgattttg ttgaatttta
    36481 taaaaagaca tgcgagaagt ttggcaatat agttggaaca aaaaacgatg tttacatatt
    36541 gagtggtgaa ggaatattag ggcttgaagc agcatgtgca tcacttaccg aaaaaggaga
    36601 tagagtactt gtaattgaca atggtatata tggagaaggt tttaaagatt ttgtaacaat
    36661 gtatggtggg gagtatgtgc ttttttcaag tgaatatact aaaagtatag atattgatga
    36721 attgaaagca tttttagatg aggatagtaa ttttaaatat gcaacagtag tgcattgtga
    36781 tactccaact ggcgtattaa atgatgtaag caaaatatgt ccattactta aagaatatgg
    36841 aatattaact gtagttgatt ctgtagcagg tatggtagga gaaagattaa gtgtggatga
    36901 atctaaaata gatataatat taggtggttc acaaaaagct atttctgcac cagcaggact
    36961 tactatagtt ggaataagtc aagatgcaaa aaattgtata aaaaatagaa aaactgatgt
    37021 aataggtttt tattgtaatt taaatatatg ggaaggctat tatgaaaaaa aatacttccc
    37081 ttatacaatg ccaataagtg acataatggg gcttgataaa gcacttgata atatattaga
    37141 agaaggaata gaaaatgtat tagatagaca tgaaaagata gcttatagtg ttagaaaatc
    37201 tattgaggaa tatggtctag aactattttt agaagaagga tattctaata cagtcacagc
    37261 tataaaaatt cctgaaagta taggagcttt aaaacttact gattatatgc ttaaaaatta
    37321 taatacactt gttgcaacat cactaaatca atatatggat actattttaa gaataggtca
    37381 tatgggagag aatgcaaatc taaataaaat agagcatgta ctaaatgtct tggataaaag
    37441 tttaagtgca ttaggattca aaggaaatga aaatttgttg aatttattta ataagtatta
    37501 tttttaatag atagggggat atagtatgga agttacagct aagcaaaaat ttacaacgaa
    37561 agacatggtg gaaacctcac tattaatagc attagtattt attgcaacta aatttataaa
    37621 tataagatta ccaatatcta taaatggtgg gcttgtgcat ttaggaacag caatgttgtt
    37681 catatcagca atagtatttg gaagtaaaaa aggtgcttta tcaggagcta ttggtatgtc
    37741 actttttgat ttaatatctg ggtggacttt atgggcaccg tttaccttta tagttagagg
    37801 agttatgggt tatttacttg gaaagattgc atgggcaaat ggtaataatg ggaataattt
    37861 tttaatcaat gtaataggaa tatgtgtctc atcaatttgg atgttatttg gatattatat
    37921 aacagaagtt atactttatg gtaattttat agtacctctt acttcaatac caggtaactt
    37981 aatgcaagta ttgataggtc ttataatagc gcttcctatt tctaaggtat tgaaaaaatg
    38041 tataaaataa atataaagtg aactaaaatt tatgatagca taaattatac tgtataatat
    38101 taagatggag tacataatta aaaaatttta gtatagaggt tagttcacaa tgttatcagt
    38161 tacaagatat tcaatcaaaa atgttcgttt aaagccgtgg gtaaagttta tttggtattt
    38221 tgaaacaaaa tcaaatatac tgttaaataa taagttactt cctacagaca gtatagatat
    38281 tattcttaat ctttctgatg ttatggaata taaaattgaa aatcaagact atactgctag
    38341 taatatgcat tttaatggaa taagggataa acatggattt attattcaac atggaaatat
    38401 acgtgttata gggatttctt tttatccatt tggtctctat ccatttttaa agataccaat
    38461 ttcagagttt aacagacaaa ttgttgactt agaagcagta tcagaactat ttgccaaaaa
    38521 attggaagag tcattaaatc caacacaatc agtagaaaaa attgttttat atttagaaga
    38581 aatgttatta tccatattag aagaagattt aatttcaaat aaatctgtaa agcttctaaa
    38641 ttcatttata tatgataata gatatagcaa tataaaagca ttttgtgata atacaaatat
    38701 aaatattaaa acattggaaa gaatctgttt aaaatataca gggtatacac ctaagatttt
    38761 aaagagaatt tacagattta aaatggcaag tagtcaactt atttataatt ataaagataa
    38821 tgaacttttt gattttattt atgaaaatga gtactatgac caagcacact ttataaaaga
    38881 atttaaaaaa ttttcaggga cgtcaccaat taaatttatt ggtgagaata agacgattaa
    38941 agaaaatata aagtacaact atttataatt atactgtcga ttttttacaa tacctccaat
    39001 ttattttttg atatgatata ggaaaataaa ttggaggttt tacatatgaa aattattaca
    39061 gaaatagttg agtttaatgt ggaagaaaat ttatcaaaag aagagtttat agaaattgta
    39121 aatgacttag aattaaaatt tcattctatt caatctggtt ttattgatac tgagctttta
    39181 tatgatgaaa agtctcaaaa gtggattatg atacaacatt gggaatctat agaagacctc
    39241 aagagtgctt ctagtaaaat gtttaaagat tcttcaactg agaaatttag aaaagcttta
    39301 aagaatcaaa ctgttaagat gaatataata ccacaaatta aaagttggaa attatagaac
    39361 tgaatgtaaa agatagttaa atagtatcaa actaaattag ccaaataaca ttttgtattt
    39421 aaattgttat ttggctttta gtgtttactt taatatgagt gttatatact agaaaaacca
    39481 acaaactgtt agagtgtgga ttataataat gtcatatttg ggagctaaaa ggcttaatac
    39541 tgatttgtca atatgtcttt ttagcatgtt gaacaggtat attgccatgc tttcattatt
    39601 ttattttttt gccttatact ggactacatt tgcagtttcc taatatattt ggctcatatt
    39661 taaatgccat tatctaaaag tactattgct gtacgagagg caataggcat tttagaagaa
    39721 ggaggagttg agctaaagta tacatcataa tctcttaagt tttctttttt tgtgtttcct
    39781 ccaaactcta ccatatcagt atttaatatc gttttgtagt ttgtattttg aaactttgaa
    39841 atatcaactg attgtattag attagggtgg aaattaaata taaataaata tttgccctta
    39901 ctgtacacca aaatcttttt atcattatcc atatgtataa gttcatgact attttggata
    39961 aaaatatcac tgtattttgt aagttcgagc atagatttat caaaatttaa taattgttta
    40021 tactttaagt tgttatcttc tgaaaggctc cattgtcttc tggcatactt atagctccaa
    40081 ttgttgcctt ctcgtggaaa atcaatccac tcaggatgtc caaattcatt tcccataaaa
    40141 tttagatatc cttcacctgc tagagaaaaa gttattaatt taattaattt aataagagat
    40201 attgctctat tgataacatg gttatttgag tttatttcca tattccagta catttccttg
    40261 tcagctagcc aaaaaataat tgttttatcg ccaactaatg cttggtcatg ggattcacaa
    40321 tatcctatat ttttttctcc aggtcgtcta gtagtaagtt cataccacat ttttcccaaa
    40381 tcccagtctt catcagataa gttagagata gtttttatcc aaaaatcagg tactcccatt
    40441 gcgagtctat agtcaaatcc aattccgccg tcttttatag ggatacacat accaggcatt
    40501 ccactcatat cttctgcaat acttatcgaa ttaggtttaa tttcttttat taattcattt
    40561 gcaaattgga gatatgttat agcttcaata tctgtattca tactaaaata tttttcataa
    40621 ctatcaaatg aaactccaag accatggtta tgatataaca ttgaagtcac accatcaaac
    40681 ctaaagccat caaaatggta ctcatttaac caaaatttta tatttgaaag caaaaaatgt
    40741 attacttcag gtttaccata gttaaaaagt tttgtacccc aagcaggatg atttcccttt
    40801 gaacctgagt ggaaaaattg atgttcactt ccatcaaact catttattcc ttctaaagta
    40861 ttctttacag catgagaatg aactaaatcc aataaaacag caatccccat tgaatgagca
    40921 gtgtttatga ggttttttaa gtcttcagga gtaccaaacc tagatgagat tgcataaaag
    40981 tttgaaactt ggtatccaaa agatgcataa tatggatgtt ccataatagc cataagctga
    41041 atggtattgt atccagcttt ttttatctta ggaagtattt tttcagtaaa ttcattataa
    41101 gttccaatag attcgctttc agtagacata cctatatgac attcatagat tagtggagaa
    41161 gttatgtttt ttaagtcaaa attattgtca gtccagataa atggagtttt tggttgccat
    41221 atttgtccat taaatccacc aaaatctttt tgaattactc tttttatata taatggtata
    41281 cggtcaaatg tttttccatt tgcagtaact tgtaccttta cctctgattt gtgaggtaag
    41341 ctattttgac caggaataaa tatttcccaa ttaccagacc caatttttct tagaggatga
    41401 gattttctat tccaattatt aaagtcacca attagtgaca aactatctgc atttggagcc
    41461 cattccctat agaagaatcc atcatttgtc tgatgaaatc catagtaaag atgagcattt
    41521 gcaaaagaac taaaatcttt atagtttttc aatgtttttg atttcatatc ctcataatgt
    41581 ttcattcgct tttttatatc agattcaaat ggttgtaggt atacatcaat atctaaaatt
    41641 ttaaatcttt tttgactttt cattgtattt ctcctccata ataaatatta catttaaaaa
    41701 ataatattta tctcatacta tcttctctgt ataattgata tatattatat aatcctaata
    41761 aaaagcttaa gccaccaatg attaatttca taatttacct ccctagtatt tatacttata
    41821 tatcgtattc atcaaaattt aaaagtttgt ttaaaactct tttaataata tcataatcaa
    41881 agcctttata ggaaagatgt tgagaaattt tttgatatat tttcttttta tcttctttct
    41941 ttactctttc ataccttttt tttgctagat acatagcatt ttcaaattca gtgtccttgt
    42001 ctaattcaga tactgcttca ttgattgtac ttctattgat acctttatta tataaattct
    42061 gcttaattct gtttttgcca catttattta ggttaacatt tgtgttaaca attttttgag
    42121 ctaatacact gtcatttacg agattataac ttttaagaaa atctatgacc ctctcaatta
    42181 tattttcatc aaattctgat gatagttttt cttttaattt tttttcagat tggtcagcat
    42241 gagataaaat atttaatgct ttatttttag cttttatata catttcatca tctaaaatag
    42301 gttttaattc ttcttcattt atttcattcc ctttttttaa attaaatgta taaacaagtt
    42361 ctttaaatat agcaataaaa aatttatcat ctacataaat attaactcta tcatcatttc
    42421 ttttttgttg ttctattttt gtaattatac ccataagtcc tcctttttta tatgtaatta
    42481 tattaacaaa agcaactaat aaaatttaac aaaaaactta tatttgtatg ttatatt
//
LOCUS       DAMKPG010000027        39630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMKPG010000027 DAMKPG010000000
VERSION     DAMKPG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.029"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            118..696
                     /locus_tag="PC621_001520"
     CDS             118..696
                     /locus_tag="PC621_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF4108682.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            726..872
                     /locus_tag="PC621_001521"
     CDS             726..872
                     /locus_tag="PC621_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF4108683.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            1177..2556
                     /gene="scfB"
                     /locus_tag="PC621_001522"
     CDS             1177..2556
                     /gene="scfB"
                     /locus_tag="PC621_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF4108684.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            2585..3082
                     /locus_tag="PC621_001523"
     CDS             2585..3082
                     /locus_tag="PC621_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF4108685.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            3380..5044
                     /locus_tag="PC621_001524"
     CDS             3380..5044
                     /locus_tag="PC621_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF4108686.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            5057..7507
                     /gene="recJ"
                     /locus_tag="PC621_001525"
     CDS             5057..7507
                     /gene="recJ"
                     /locus_tag="PC621_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF4108687.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            7520..8032
                     /locus_tag="PC621_001526"
     CDS             7520..8032
                     /locus_tag="PC621_001526"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF4108688.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            8106..10313
                     /locus_tag="PC621_001527"
     CDS             8106..10313
                     /locus_tag="PC621_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF4108689.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            10338..10787
                     /locus_tag="PC621_001528"
     CDS             10338..10787
                     /locus_tag="PC621_001528"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF4108690.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            10802..11416
                     /locus_tag="PC621_001529"
     CDS             10802..11416
                     /locus_tag="PC621_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4108691.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            11507..13063
                     /locus_tag="PC621_001530"
     CDS             11507..13063
                     /locus_tag="PC621_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF4108692.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            13053..14315
                     /locus_tag="PC621_001531"
     CDS             13053..14315
                     /locus_tag="PC621_001531"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF4108693.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            14389..16176
                     /gene="aspS"
                     /locus_tag="PC621_001532"
     CDS             14389..16176
                     /gene="aspS"
                     /locus_tag="PC621_001532"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF4108694.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            16635..17867
                     /locus_tag="PC621_001533"
     CDS             16635..17867
                     /locus_tag="PC621_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF4108695.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            17881..18687
                     /locus_tag="PC621_001534"
     CDS             17881..18687
                     /locus_tag="PC621_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF4108696.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(18799..19668)
                     /locus_tag="PC621_001535"
     CDS             complement(18799..19668)
                     /locus_tag="PC621_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4108697.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            20039..21466
                     /locus_tag="PC621_001536"
     CDS             20039..21466
                     /locus_tag="PC621_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF4108698.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(21542..21811)
                     /locus_tag="PC621_001537"
     CDS             complement(21542..21811)
                     /locus_tag="PC621_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108699.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(21814..23256)
                     /gene="nhaC"
                     /locus_tag="PC621_001538"
     CDS             complement(21814..23256)
                     /gene="nhaC"
                     /locus_tag="PC621_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF4108700.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(23281..24447)
                     /locus_tag="PC621_001539"
     CDS             complement(23281..24447)
                     /locus_tag="PC621_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4108701.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            complement(24672..26792)
                     /locus_tag="PC621_001540"
     CDS             complement(24672..26792)
                     /locus_tag="PC621_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108702.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            27025..27426
                     /locus_tag="PC621_001541"
     CDS             27025..27426
                     /locus_tag="PC621_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108703.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            27469..27762
                     /locus_tag="PC621_001542"
     CDS             27469..27762
                     /locus_tag="PC621_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF4108704.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            27777..28217
                     /locus_tag="PC621_001543"
     CDS             27777..28217
                     /locus_tag="PC621_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF4108705.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(28309..28782)
                     /locus_tag="PC621_001544"
     CDS             complement(28309..28782)
                     /locus_tag="PC621_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF4108706.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(28782..29570)
                     /locus_tag="PC621_001545"
     CDS             complement(28782..29570)
                     /locus_tag="PC621_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF4108707.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            29898..31142
                     /locus_tag="PC621_001546"
     CDS             29898..31142
                     /locus_tag="PC621_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF4108708.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            31465..32562
                     /locus_tag="PC621_001547"
     CDS             31465..32562
                     /locus_tag="PC621_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF4108709.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            32553..33251
                     /locus_tag="PC621_001548"
     CDS             32553..33251
                     /locus_tag="PC621_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4108710.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            33254..34318
                     /locus_tag="PC621_001549"
     CDS             33254..34318
                     /locus_tag="PC621_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF4108711.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            34501..35205
                     /locus_tag="PC621_001550"
     CDS             34501..35205
                     /locus_tag="PC621_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108712.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            35198..36520
                     /locus_tag="PC621_001551"
     CDS             35198..36520
                     /locus_tag="PC621_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4108713.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            36707..38080
                     /locus_tag="PC621_001552"
     CDS             36707..38080
                     /locus_tag="PC621_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108714.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(38453..39391)
                     /gene="pdaA"
                     /locus_tag="PC621_001553"
     CDS             complement(38453..39391)
                     /gene="pdaA"
                     /locus_tag="PC621_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF4108715.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT        15130 a         4557 c         6746 g        13197 t
ORIGIN      
        1 ttatataggt ctttttttga ccagttttgc atttttaaca actagttttg taaagtatat
       61 tgaattttct caaaaaagaa tgtactctat atatgtaaaa tttttttgga ggtaaaaatg
      121 tttaaaagat atgctgaaaa aatgacatct gttttaattt gtaataatat gatagataat
      181 aatgaatcta aagtctattc ttatggattt gaaatattaa tagctttcat agttaatata
      241 accaccatgc tgtttatagg ttttttattt ggtaaattta catatgtatt atttttttta
      301 atgtgttatt gtccaataag acaattttca ggagggtatc atgcagacaa ttattttaga
      361 tgcttattaa cctttatttt tataatttta tctacaatct taattataga aaatataaat
      421 atagacttat ttaaaaatat tattatgatt attgcaagtg ttagttgggt tggaatttgt
      481 gttctgtgtc caatagaaca taggagtaac cctataagtg atagggaaaa gtttgtttat
      541 aagaaaactg ctatatttat atctacagta gtgttattga taacaatttt aagtctttct
      601 ttgagtatat ttgtagatta tttcacatat agcgcatttg ccatgttttg gatttttgtg
      661 atgttagtat taggtaagtt aaaagctaaa gtataagtaa aatatgtatg agggagagtt
      721 aagttatgaa aaagtttatt gtaagattta tgaagtttgc tagctcattg gcacttagca
      781 ctgctatttt gtcagctaat agtacctgtc cttggattat tcatcagcct aaagtaccca
      841 aagaaatcag caatctaaag aagacaaatt aaatacaagc acttatttat acaaaattat
      901 caggaaaaaa gaaattatta tattttaata agattaaaac aatataaaat cagatacaaa
      961 atttatttta tttattttat aggagcctaa tacctatttg gtaatatatt agtgcgccaa
     1021 tttaggggga tatagtgtat ttaatgaaat gacttacgga gagtaagtca tttttgtatt
     1081 gtatgtattt tatgtaaact tatgatataa tgaagggtaa agtttaatta aaataataaa
     1141 aaactttagt atagatagta atttaggagg tagggtatga gtatgataca taaattttct
     1201 atgaatggat ataatatcgt tttagatgtg aatggtggag ctgttcatgt gttagatgat
     1261 gttgcatacg atttgttaga tttttataaa gaaaaaagta aagaagaaat attagagata
     1321 ttaaaatcaa aatatcaaga agaaaaaata aatgaagcat atgaagaaat attaaatttg
     1381 gaaaaagaag gtcttttata cacagaagat acatatcaat atcatccaag ctttgtacat
     1441 agagagcctg tagtaaaagc tttatgttta aatgtagctc atgattgtaa cttaaaatgt
     1501 aaatactgtt ttgcagctca aggtgatttt ggaggagaaa aagaacttat gagttttgaa
     1561 gttggtaagg cagcaattga ctatttgatt gctaattcag gaagtagaaa gaacctagag
     1621 atagatttct ttggtggaga gcctttgatg aactttgaag ttgtaaaaca actggttgac
     1681 tatggaagaa gtgtagaaaa agattataat aaaaatataa gatttacaat aactacaaat
     1741 ggtgtactgt taaatgatga gattatagat tatataaatg aaaatatgca taatgtagtt
     1801 ttaagtttag atggaagaaa agaagttaat gataatatga gaccaacttt aaatgataaa
     1861 ggaagttatg atattacatt accacgattt aagaaacttg tggaaaaaag agcaaaagat
     1921 aagtattatt atataagagg tacttttaca agagataatt tagatttttc aaaagatgtt
     1981 atgcattttg cagatttagg atttaaatta acctctgtag agccagtcgt tggagatgag
     2041 agtaatccat atgcacttag agaagaagat ttacctaaga tatttgaaga gtacgaaaaa
     2101 tttgctgtag agtatgcaga tagacaatta caaggagatg gatttaaatt tttccatttt
     2161 atgatagacc taaatcaagg tccatgtgta ataaagagaa taactggatg tggagctgga
     2221 aatgagtatc tttctgtaac tccaaatgga gatatctatc catgtcatca atttgttgga
     2281 aatgaagaat ttaaaatggc aaatatattt gatgaagaaa tagtacttcc agaaaattta
     2341 aaaaatatgt ttagagaagc tcatgtatat actaaagaag agtgtaaaca atgttggaat
     2401 aagttttact gttcaggtgg atgtcatgca aatgcgataa actttaataa tgacatatca
     2461 aaaccatatg aattaggatg tgaaatgcaa agaaaacgta cagagtgctc tataatgata
     2521 caagctaagt taatgttaga aggagcaacc aactaaattt atattttaaa ggagttgttt
     2581 atatatgatt agagattatt tagaagataa acctttaatt gacgaaagtg tatttgtggc
     2641 aaaatcagca gatgtaattg gaaatgttaa aataggcaaa gattcaagta tatggtacaa
     2701 tgcagtagtt aggggtgatg aaggaccaat aactattgga gaaaacacta atatacaaga
     2761 ttgttctata gtgcatggag atacagaaac tataattggt aacaatgtaa cagttgggca
     2821 taggtctata gttcatggtt gtaaaattag tgataatgtg ttaattggta tgggttcaat
     2881 aatattggat aatgctgaaa taggagaata tactctgata ggagcaggta cactaattac
     2941 atctaataag aagtttccac caggtgtttt gataatgggt tctcctggta aggttgttag
     3001 agagcttaca gaagaagata aaaaatatat tgatgaatct tatgaatggt atttagaggc
     3061 agcacaaaat caaaaatatt agtttaaaga gtggttgttt caaaacatat ttaaatctgt
     3121 tttgaggcaa ccactaattt attttattag attttgttga tattttagtt ttcaaaaagt
     3181 atttgattaa accagatata aaatgtcaaa aataaaaatt aactgagtta aataatttta
     3241 ataaaattgg tagtatatgt gataaaatta aatattaaag aggatatagg atattagaat
     3301 atcaattggt gaaataatgt catatacgat aaattaaggt gtaagttttt gggtatacct
     3361 atcttaggag tgaaaagatt tgagaataag ttatagaaat ttaaagcgat atagagaaat
     3421 tggatatgtt ttaataaagt atggttttag ttttattgta gaaagactaa atatagaagg
     3481 aattgcctat aaaataccac tatttgaccc accagaagaa ataaaaaata tgacaactgg
     3541 tgagagaatg aaaagagttt tagaagaact aggacctaca tatataaaaa taggacagat
     3601 attgagtaca agaaaagact tgttagacca ggatataatt gatgaaatat caaaactcag
     3661 agatgatgta gagaaatttg attcaaatat agctattgat atatttaaag aagaagtggg
     3721 tttaagtata gaagaaatat tcttagaatt taaagaagaa ccaatagcag cagcttcaat
     3781 tggtcaagtt tatgaaggtg tacttaaaac gggagaagaa gtaatcgtaa aaattcaaag
     3841 accaaatata gagaaaataa taaaatctga tttagagatt cttagaacta ttgcaaatac
     3901 tttaaaagat ttaaaaaagg attttaattt agatttagta cagatgattg aagaatttca
     3961 aactcaatta atgagggaac ttgattatac atttgaagcc ataaatgcaa ctaaattctc
     4021 taggatattt aaaaatagtg atgaagtata tatacctaaa gtatactcag aatataatac
     4081 taagaaaatt ttagtaatgg agaaagttaa tggtacaaag ttaagtgatg tagaaaaaat
     4141 aaggagacta ggttataaca caaaaactat tgttgaaata ggagttaggt cattttttac
     4201 acaggtatta tctcatggat tttttcatgc agacccacat ccaggtaata tatttgtagt
     4261 tgctaaaaat aagatagcat atatagattt tggaatgatt ggaattattg ataataaaac
     4321 tttaaaccaa ttaaatgaaa ttgcattagc aggtgtagaa aagaatgttg acaagataat
     4381 atatctattg atagaaatgg atgcattaaa tggagaggct gatattaagg gacttagaca
     4441 ggatttactt tatctaatac attattacta tgatatttct atagaaaaaa taaatgtaac
     4501 agatatatta aatgaactat ttaggttttt tagacagtat aaaatagtca tgcctgcaca
     4561 atttgtaact cttgcaaaga cagttataac attagaagga acaagtagaa ctttaaacac
     4621 agatttttct tttggttcta tgggaaaaga gtttatgaaa catcattata agagtaaatt
     4681 taaccctaaa aatgttgttt taagttcaag acaaaatgta gaagaaatat tactggacat
     4741 aaaaactatt ccaaagcaat taaaagcaat tttaaaaaat atagaaagaa ataatataaa
     4801 aatgcaaatt gaagatgtta aaatgacgag gttagaaaat tgtataatag agctaacatc
     4861 tcaaatatct ttgagtttag tgttggcatc tattatagta ggttcttctc ttataattgc
     4921 atctccaaat atagaaaata atatatggat aaaatttact gcaatagcgg gtttctttat
     4981 atcctttata ataggccttt gcttagttat aagaagtatt aggtcaaaat acaaaaaaga
     5041 ttagggggaa ttaaatttga aaaaatggat attgaagtat aaaggtaaga tagaggaaag
     5101 tgaatttagc aaaaaactta atatttcccc ggaaatctgc caaattttaa aaaatagagg
     5161 gatatgtact gaaaaagatt cagaaatatt tatgaatcca tctttagatt atttaagaga
     5221 ccctttttta atgaaagata tgcaaaaagc tgttgataga ataaagttag cagttgagaa
     5281 gaacgaaaaa atttggattt acggagatta tgatgttgat ggtgtatcat ctacatccat
     5341 cttgtgttta tattttaaga gtataggtta caatgttgat tactatattc ctaatagatt
     5401 agaagaaggg tatggtataa atgaggatgc tattaagctt ataagctcta ggggatgtga
     5461 cttgattata agtgttgact gtggaattac atcagtgtct gaggtcaata cagcgaatga
     5521 tttgggcata gatgttataa ttacagacca tcatgaatgt caaagtgaaa ttccatcagc
     5581 ctttgcagta ataaatccaa aacaagaaga ttgtaattat ccatttgatt cactatgtgg
     5641 atgtggtgta gcatttaaaa tgattcaagc cttgactcca aaagaagaat ttaaaactag
     5701 tatgtataat tatttggaaa tagtaacact agcaactatt tgtgatattg ttccacttat
     5761 agatgaaaat agaataattg taaaaaatgg tttaaaatca atgaaagaag gcaaaaatat
     5821 tggattaagg gagcttataa aagtttgtgg tgtagaaagt gataagatag gttcttctca
     5881 tataggtttt gcaataggtc ctaggataaa tgcttcaggt cgattgggat attcttactt
     5941 aggagtagag ctatttacta ctcagtcaca agaagaggca gtagaaatag ctagtatatt
     6001 ggaagaaaaa aataatgaaa gacagatgat agaagctaag atgtatcatg aagctgaaga
     6061 aatgctaaag tctaatagca gatacaatga tgacaaagtg ttggttttag ccaaagaggg
     6121 ttggcaacat ggaataatag gaattgtagc atcaaagtta actgaaaaat actacaaacc
     6181 aacaattctt ctaggtatag aaaatgggga ggctactggt tcagcgaggt caataaaagg
     6241 atttaatata tttgaagctc tcataaagtg caaagacttg atgactaagt ttggtggtca
     6301 tgaacaagca gcagggttat ccttggattc agacaatgta gaaattttag caaatgaaat
     6361 aaataaattt gcagattata atttaactga agatgatatg atagagaatg taaatgtcga
     6421 gtttgaatta caagaaaatg taataaattt aaatcttgta gaagaattac ataagttaga
     6481 accctttgga ttaaataatc ctaatcctag atttatagtt agaaattata ttttaaaaga
     6541 tttgaaagtt ataggtaaga atcaacaaca tctgaaatta agtatagaaa aagagaaaag
     6601 ctatgaatgt ataggattta atatgagtca tttaaaatca atgtataaag taggagacaa
     6661 agtagatgta ctattccaat tagatgaaaa taactatatg ggaaatagga aagtacaatt
     6721 tttacttaaa gatatacgat tggctcgacc taaaagtgca agtaatgaca aactttcatt
     6781 gaaattaatg tcaaaaataa taccaaaaga tactcaaagc ctgtataata tatctgtgtc
     6841 tgattttgaa ttatttgatg gaaatacaga tataaatata tttgattatt ttgaaaaaga
     6901 tacactaata atatctaatt ctataaatgg attttataga gcaatgtcag atatatcact
     6961 tatagatttg gattttaaca taaattacaa tattattgaa gatgttagca aaaatactga
     7021 taaattagag cttatatttt ctcctaatat tgataaaata gacttaaaaa gatataataa
     7081 tattattctt tatgattatt tatacaataa aggcgaatat tcgtatatat atgagaataa
     7141 aagagaagaa agtgaaatta taaaatatta taataaaaca gacctattat atttaaagaa
     7201 tgtagttagt aatatagttc catcaagaga tgaatttata acaatttata aacaagcact
     7261 tataaaaaaa gaaattgatt tggatatggt aaatataagg gaaactttta atgtaattcc
     7321 attaaagttt tttacgatat taaatgtatt tagagaatta aatttacttg actttaattt
     7381 aaattatgaa aaaaattctg tactcataag aatattgcca aaaccacaga aaaaattaga
     7441 cttaaatgag agtttaatac tcaataatct aaaaaattta gaaaaacaat acaatagcag
     7501 ttattaggag gcgtttccca tggatttaaa aaactttata aggaatattg atgatttccc
     7561 aaaaccaggc atagatttta aagatgtgac aactctattt aaagatggag atgcatttaa
     7621 atatgcagtg gattctatag ttgaagaatt gaaagataaa gatgtagatt tagtaatagg
     7681 gccagaagca agaggatttt tgatgggtac acctgtagca tatgctttag gagtaggatt
     7741 tgttccaatt agaaaacctg gaaagttacc aggtgaagtt gagagttatg agtatggatt
     7801 agagtatggt acagatactt tagagataca taaggatgca attaaaaagg gtcaaaaagt
     7861 agcaatagta gatgatttat tggctactgg tgggactatg gaagccgctg ccaagcttgt
     7921 agaaaaatta ggtggagaag tagtttctat gcaatttttg attgagctaa aattcttaaa
     7981 tgggagagaa aagttgtcta actatgatgt taattctctt ataaaatact agtactttta
     8041 taatagttgg tataacacca actattttca tatatattaa aattttatac aaaataggtg
     8101 gttatatgca tgataaagaa ttacaagaaa taattgaaaa aataaaagtt tatgcaccta
     8161 atgcaaattt tgaaatggtt gaaaaagcct ataatcttgc aaaaaaagcg catgaaggac
     8221 aatatagaaa atctggggaa ccatacttta ttcatccatt agcagttgca aatattttaa
     8281 tagagatgga attagatatt gaaactataa cagcaggttt acttcatgat gttgtagaag
     8341 atacagaata tacctatgaa gacataaaaa aagaatttgg caaagaagta gcggatttgg
     8401 ttgatggagt aactaaactt ggtcaaataa aatatcgttc gaaagaagaa actcaatctg
     8461 aaaatttaag aaaaatgttt ttggcaatgg cgaaggatat aagggttata cttataaaac
     8521 ttgctgatag attgcacaac atgagaacat taaaatacat gcctccagaa aaagcaaagt
     8581 ataaagcaaa agagacactt gaaatttatg gtggtatagc tcatagatta ggaatttcca
     8641 agataaagtg ggaactagaa gacagagcat taagatttat ggacccagaa gggtattatg
     8701 atttagttag tagagtatca atgaagagaa gtcaaagaga ggattatatt caaggaattg
     8761 ttgaacttct taaagataag tttaaagatt taagtatacc atgtgatgta tatggtagac
     8821 caaagcattt ttacagtata tatagaaaga tgcaaaaaaa acataagact tttgaagaga
     8881 tttttgattt aacagcagta aggatattgg ttgataatgt aaaggattgc tatgcagtac
     8941 ttggtatagt tcatacatta tggagaccta ttccaggaag atttaaagat tatatagcaa
     9001 tgccaaaacc caatatgtat caatcattac acaccactgt tgtaggtcct gatggagaac
     9061 ctttagagat acaaataaga actcatgaga tgcataatat agctgaaaat ggtatagcag
     9121 cccattggaa atataaagaa ggtatctcta gcaaagagtc aaaggttgaa gaaaaacttc
     9181 aatggttgag acagatgatg gagtgggaaa aagatttaaa agacccacaa gagtttatgg
     9241 atgcacttaa agaagatgta tttaatagtc aagtttatgt atttacgcca aaaggagacg
     9301 taattgaatt accagcagaa tcaacaccta tagattttgc ctatagagtt catacaaatg
     9361 taggtaataa atgtgtagga gctaaaattg atggaagaat agttcctatt gattataagc
     9421 ttcaaaatgg taatattgtc gaggtactta catcatctaa ttctaatgga ccaagtaggg
     9481 attggcttaa tatagtgaag accccaaatg caaaaagtag aattagacaa tttttcaaga
     9541 aggaaagaag agaagaaaat gttgaccgtg gaaatacaat attagaaaga gaatttaaga
     9601 agcatgatat accacaaaaa gaccctcttg ttgaaaaata tatggcatta gttgctaaga
     9661 aatttaatca gccaagtgtt gaggacttaa ttgccactgt aggttatggc ggtataatgt
     9721 catctcaagt ggtatctaag gtaaaagacc tatatttaaa agaggttaaa aaagctaaaa
     9781 aggaacaaca gatacaagaa gaagatttaa ataaacacaa tataagtgaa acagaataca
     9841 gtaaaaagag aaagaaaaac gcatcacaag gaattatagt aaaagggctt gacaatatct
     9901 tagtaagatt tgctaagtgt tgtaatcctc ttccaggaga tgaaattgta ggatatatta
     9961 ctaagggtag gggagtagcc atacacagag ctgattgccc aaatgcaaac aataatggtg
    10021 atttcttcca aaatagattg gtggaggttt catggagttc agcaggaaaa ggtaagtttg
    10081 aagcggaaat tcaaataaaa gctattgaca gaagaggaat tgttaatgaa ataactcata
    10141 ttgttgcaat ggataaactt agtttaaacg gaataaatgc aagaaaaggt aaggatagta
    10201 ttgtaagtat aaacttactt gtagaggtta atgacacaga ggaattaaaa attctaatga
    10261 aaaaattaaa agcaattcct ggtgtagaaa gtatttatag aatgacaaat tagagttaaa
    10321 ataataggga ggaaactatg agagcagtag tgcaaagagt atcttcatct aaagttacag
    10381 tagatgaaaa cactataggt caaataaata aggggttact agtattgtta ggagtgactc
    10441 atgatgataa atcaagtgat gtagattata tgattgataa aatattgaat ctgagaattt
    10501 ttgaagatga aaatgacaag atgaatttat ctctaatgga tataggtgga gagcttttag
    10561 tagtatctca atttacttta tatggagatt gcagaaaagg aagaagacct ggatttagta
    10621 atgcatctag accagaactt gcaaataacc tttatgaaga atttgtaaaa aaagcaaaag
    10681 ataaaggtgt gacagttggt acaggtcaat ttgcagctca tatgatggta gagttaacta
    10741 atgatggtcc agtaacaata ctattagact caagtaaatc tttttaaggg ggaaaacttt
    10801 aatgattata aaaaaatttg tagatagtta ttttggagta aatacttatg tattaggtga
    10861 tgaaaagact aaaaaatgtg cagttataga cccaggtgga agtttagtag agacattaag
    10921 ttttgtaaaa gaaaatgaat taaatattga atatattatc cttactcatg ggcatggaga
    10981 ccatatagga tatgttaagg atattaaaga aaaaactggt gctaaagtag ttgcacatgt
    11041 tgatgaaaaa gaattattaa atgataaaaa taaaaatcta agttatacaa tgagatgtgg
    11101 agcgcaagag tttgatgcag atatctatgt gagtgataaa gacaagcttg aacttggtga
    11161 attaaaaatg actttcttgc atactccagg acatactcaa ggatgtatgt gtataagagt
    11221 tgaagatgaa atgttcacag gagatacttt gtttgctgga agtattggta gaactgattt
    11281 atatagtgga gactttaacc aaatggaaaa atcacttaag aaattatgca aatatgaaga
    11341 tgctgttaga gtatatcctg gacatggacc aaccagtact ctaggggttg aaaagaagac
    11401 aaatccttac atgtaatttt atattagtat ttaaaaaagt ataaaatatt taataactat
    11461 attagtgagg gggttcttct actgttaaat agaaggagtt aagttgatgt tgtatgtttt
    11521 tttaaaagga catgattata agtatgaagt agcagaactt ataaaattgt ttaccagtga
    11581 atttaagttt aaagactcta atgctaacaa taatatagag tcaaagcatt taataaatag
    11641 attgatttat gagaatggtg tattattctc aagtactgaa tactatgaaa atggaaattt
    11701 aaagtacgag tcaatacaga atatagaaaa tatgaatctt gaatttttag atggatttga
    11761 tagtagcaaa acgattgaag aatttcttaa tagactagat agtgaaagta agaggaactt
    11821 taaaaagctg tgtaaagaaa atatcaaaaa aagcatgttt gtagtgctta aacaagtatt
    11881 taattcttat gtaccatggg gaattttgac tggtattaga ccagtcaaaa ttgtccacaa
    11941 tttaatggat aaaaagctag atgataatag cataagaacc attctaaaag ataattattt
    12001 tataatagat aagaaaatag atttagctct tgaaatagct aaaagagagc gattatttat
    12061 gtatcctata gataaaaata aaatttctct ttatgtaagt attccatttt gtccaactag
    12121 atgtgtatat tgttcatttc cttcaaattc attaaaacaa tttggacatt taaaaagaga
    12181 atatgtttat aaattgattg aagaaattaa ggggtttgcc aaagttatta aggagactaa
    12241 gaaagaagtt gaaactctat atattggtgg tggaactcca actacacttg atgaggaaga
    12301 attagatttg cttataaatt cattgttcaa tgaattagat ttaagtaaaa ttaaagaatt
    12361 tactgtagaa gcaggtagac ctgacactat tacagaacaa aaactaagag tgttaaaaaa
    12421 acacaatgtt agtagaatta gtataaatcc tcaaactatg aatgatgata ccttggtaaa
    12481 gattggaaga gagcatagag tatctgattt agtagattgc tttaatatgg ctcgtagaat
    12541 gggatttgat aatataaata tggatataat tttaggtctt atagatgaag aattaaatat
    12601 ggtaaagaac acattggagg agataaaaaa actttctcct gaaagcctta cagttcatac
    12661 attagccata aagagagcat ccaacttaaa tataaatatg gataggtata aagaacatct
    12721 aacacaatat gaagaaatgg taaaaatgat agatttatca atgaaatatg caaaagatat
    12781 gggattaaat ccatattata tgtacagaca aaaacatatg ttgggaaatt tagaaaatat
    12841 aggttatgct aaagaaggat atgagtgcat ttataatata cagattatgg aagaaaaaca
    12901 aagtaactat gcattaggtg caggtgctat atcgaaattt gtttatgtag atgaagatag
    12961 aattgaaaga gtagaaaatg taaaaaatgt tgaacaatac attgaaagag tagatgaaat
    13021 gataaaaagg aagaaagaag aggtatataa aaatgttgac taaagctcct agaggaacta
    13081 aagacataac tccaaaagag gcttacaaat ggcgttatgt agaaaataaa tttagagaaa
    13141 tatgtgcttt atatggatat gaagaaatgg taacacctat atttgaacat acagaacttt
    13201 tcaagagaag tgttggagat actacagata tagttcaaaa agaaatgtat tcttttaaag
    13261 ataaaggtga tagagaaatt accttaaagc cagaaggtac agcaggtgta gtaagagcat
    13321 ttatagaaaa taaattatat gcagatactc aaccaacaaa attattttat gtgacacctt
    13381 gttttagata tgaaagacca caagctggta gacaaagaca atttcaccaa tttggaatag
    13441 aagcacttgg aagtgatact ccttcaatgg atgctgaaat aatcgcacta gctgttcaat
    13501 tttttaatga agttggatta aatgatttgg tagtgagtat aaattcagtt ggctgtccag
    13561 tttgtagaaa agagtacaat gctttattaa aagagtatct tgactcaaaa gctgatatat
    13621 tgtgtgatac ttgcaatgag agaagagaaa aaaatccaat gagggttata gactgtaaaa
    13681 atcctacatg taaagaaaat atcaaagata taccatttat tgcagaccat ttgtgtgatg
    13741 attgtaaatc tcactttgat aaacttcaag aatacttaaa ggaaatgaat ataaactttg
    13801 taattgataa aacaatagtt agaggcttag attactatag aaaaactgct tttgaaatta
    13861 tatctaatga tataggagct caaagtactg tatgtggtgg aggaagatat gatggtcttg
    13921 ttgaacaatt aggaggacca aaaggtataa gtggaattgg gtttggacta ggaattgaaa
    13981 gacttttact tacacttgaa ggtaatggaa ttgaaataga aaaccctcaa tctacggata
    14041 tatttatagt aactataggt gaagaagcaa atacaagaag ctttaaatta ttaaaagact
    14101 tgagacaaaa tcatataagt gcagataaag accatataga gagaagtgtt aaagcacaat
    14161 ttaaatattc agataaaata aattcaaaat ttacaatagt gattggagat gatgagctta
    14221 aaaatgacac tgcgacatta aaaaatatga aaacatcaga gcaaactact gtaaaattaa
    14281 gtacattagt tgaagaatta aagcaaaagc tgtaaaataa gaaagtatga tgttttgtgt
    14341 attagataaa tatatagcat taaaattaaa taaaatggag gtagtatctt ggaaacttta
    14401 aagggtttaa agagaactca ctactgtgga gagttgagag aaaaaaacat aaatgaagaa
    14461 gtcgtactta tgggatgggt acagaaaaaa agaaatttag gtggtcttgt atttgttgat
    14521 ttaagagata caagtggatt atgtcaaata gtttttgata cagatgtaga taaagaagct
    14581 tttgaaaaag ctgaaaaatt aggggctgaa tttgtaatag ctgtaaaagg aaaagtatgc
    14641 gaaagacaat ctaaaaaccc taatatgcca actggtgata tagaaatatt tgcaactgaa
    14701 cttagacttt taaacaagtc agaaactccg cctatttata taaaagatga tgatgatgtt
    14761 tctgaagcac taagattaaa atatagatac ttagatttaa gaaaaccatc tatgcaaaga
    14821 aatttaaaat taagacataa agtaatgaat attacaagaa attacttatc aaacaataga
    14881 ttttgcgaaa tagaaacacc atttttaata gctccaacac cagaaggagc aagagattat
    14941 ctagtaccaa gtagagttaa tccaggaaaa ttttacgcat taccacaatc accacaatta
    15001 tataaacaat tattgatggt aagtggtatg gataggtatt tccaaatagt taaatgcttt
    15061 agagatgaag atttaagagc agatagacaa ccagagttta cacaaataga ctgtgaaatg
    15121 tcttttgtcg agcaagaaga tgttatgagt atgattgaag gtttgcttga agcgatattt
    15181 aaagaagttt tagatgtaga attggcactt ccacttccta agatgactta tgcagaagct
    15241 atgtctaaat atggttcaga taaaccagat actagatttg gatatgagtt aacagatata
    15301 tctgatgttg tttgtaactg tggatttaaa gtgtttgctg atgctacaca accaggaaaa
    15361 agtgttagag gtataaacgt aaaaggcaaa gctgacgact ttactagaaa acaaatatca
    15421 tcactagaag aacatgctaa gacatataga gcaaaaggtc ttgcttggat gaaagtagga
    15481 caagaaggag taacttctcc aatagcaaaa ttctttaatg aagaagaaat gaacgctata
    15541 cttacaaaaa tgaatgctga agtaggagat ttacttttat ttgtagctga taaaaactca
    15601 atagtatttg atgctttagg acaagttaga cttgaagtag caaataggct taatctattg
    15661 gataaaaatg tatataactt actatgggta actgagttcc cagtttttga agaagatgaa
    15721 gaaacaggca cattttcagc aatgcatcac ccatttacat cacctatgga tgaagattta
    15781 gacaagttag aagaaggtga taagtcatct ttaagagcga aagcttatga tattgtattg
    15841 aatggttatg aaataggtgg aggaagtgtt agaatatcaa attctgatat tcaatcaaga
    15901 atgtttaaag cacttggttt cacagaggaa agagctaatg aaaagtttgg ttatttatta
    15961 gaagcattta aatatggtac tcctcctcat gctggtctag cttttggtct tgatagactt
    16021 gttatgttac ttgcaggaac tgataatata agagaagtta ttgcattccc taaaaatcaa
    16081 aatgctgttt gccctatgac aaatgctcct acattggcgg aagatgagca attagaagaa
    16141 ttaagtataa aagttgatat aaaagataat gaataatatg tagttgcaag aaactaaaat
    16201 gtaaaaagtc tggatataaa gtatatttat attcagactt tttttattag aattatttta
    16261 tattttttct ctatatattt tcataaatta tccttgtatt ctcagccaat ataattaaaa
    16321 tactatgttt aaaaatttta cataaacttg attgttttag aaattacttc tgtaatgagt
    16381 acacgatttt aattaaagaa tatataacaa atttgaaata agtctaatat atgacttaat
    16441 agattaaatt aaaataataa atttggacta tttatctatt tataacttaa attatatcaa
    16501 attgataaca aactagatat tatacttttt tgtagaatag aattacaatt atataaagaa
    16561 aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga aattattctt ttataaagaa
    16621 agggagggat aatattgcaa gagagagaag aattagtttt aaatccagtt ccaaaagagg
    16681 aaagagttgg atggttagct ccattgttta atatgcttgg ttctaacata gctatatctg
    16741 aactaatggt tggaggaact ttaattttag gaatgactct ttctaatatg attattacat
    16801 caattatagg gaacttaata ttagttgcaa tcattatgat acaaggttat ataggttaca
    16861 aagaagggtt aaatacgtat gtattagcta agggggcatt tggagagata ggaggaaagt
    16921 atcttatatc tctattactt ggtataacta gttttggatg gtttggtgta caagcagggg
    16981 ttgcaggact atcagtacaa aagatattcc caagtgtaaa cctaacatta gttacagtta
    17041 tacttggatt atttatggtt gtttttgcac tgtatggctt taaagctatg gctaaattta
    17101 attacttagt tataccacca cttatgatat taatggtgtg gggagttttt aaagcttttt
    17161 ctacttatgg agttgaagct atttataatt atactcctca aacaactatg agtatggttg
    17221 aaggtcttaa tattgtagta ggtcttgtta tagttggtgc aattatatca cctgaccagt
    17281 taagatatac gagacgagta aaagatattt ggattatcag ttttttaggt ctagggatta
    17341 tttcattatt ccaacaagtt gcagctggtg taatgtctat gggagcacct acttgggata
    17401 taacagaagt attggctaat ttaggttttg gatgggtagc gtttgttatt ttaatacttg
    17461 catcttggtc aacaaacgta tcaaatgctt attctggtgg attagcatta aaaactattt
    17521 ttccaaatgt aaaaagaaat gttttgactt tagttgcagg tctaataggt acaataatag
    17581 cagctactgg tataatattt aaatttcagt catttttatc tttcttggga atcgcagttc
    17641 ctgcaatagc aggaattatg tggtgtgagt attattttat acaaggaaga acttataagc
    17701 atagagaagg aataaattgg attgcagtaa taagctggtt aataggtttt gcagcgtcgt
    17761 attatagttc aaaaataaat ttcttaatac ctccgataaa tggaattgta gtgagtatgg
    17821 ttatttatta tatattaatg aaatgttttg gaatcaaaga taaataaagg gggcaattac
    17881 atgggtcgta aggtgaaaat aggtataatt caacaacaca gtgtacttgg agatgttaaa
    17941 aaaaatatag aaaaagcagt agagatgata gatgacttag gaaaacatgg ggcagatata
    18001 atttgtttac cagaactatt tgctacagga tataatttag agtctttagg tggagttaaa
    18061 acattagaat taataagaga acataataag tacatagagg aatcaatgtc agaagctgct
    18121 aaaagaaata atgtatacct gatatctcca tatggaacct tagaaaaagg gtctacacat
    18181 gtttataatt cagcagttat atttgataga aaaggtaaga ttatgggaga atactgtaaa
    18241 aatcatctgt ggtcattaga agcagtatac tttaaaggag gggaaaaagt agaggtatat
    18301 gatgctgatt ttggtagatt tggtgtaatg atatgttatg atgcaggttt tccagaagta
    18361 tccagagaat tgacattaaa aggttctgaa ataatattta taccatctgc atggagaata
    18421 caagatgaag atatgtggga tttaaatgta tctcaaagag ctttagagaa cactgtatat
    18481 acagtaggtg taaatttagt aagtaatgac tctaatttaa tactatttgg aaagagtaaa
    18541 atatgcaatc ctagaggtac tgtaataact caattagata catacaggga aaaatatgta
    18601 ttatcagaga ttgatttaga tgaaataaaa aagtataggg aagatatacc ctatttgaaa
    18661 gacagaaaaa gtagctataa catataattc tatactataa tacaagttca atataaaatg
    18721 ataaaaagag gatgtatcaa tattaagatt aaatttatta tattttgata tatcctcttt
    18781 aatattatca actttgattt aaatattttt aaaatagttg ataagaaagt ttttgaacaa
    18841 caacacagct ttagattcat agttttcgct aaaccatgaa agatttaaat atcttttaaa
    18901 ttcaatatct ttgatatgta acaacttaat ctcagggtct tgagataatc cccatgattt
    18961 tccacatata aatccaactc cttgaagtga ttttataaga gcataaattg tatatggtga
    19021 atcagactca aatgctattt tgggtttgaa attagctgat tcgcacagga tatcaattac
    19081 ctctctataa ttctcaccct tcgttataac tataaagttt tcatctgaaa cttcgcttag
    19141 atatacttca tcctttaatg ataaaggatg gtttattgaa actcctaata gtatttcttc
    19201 gcatgttaaa gttatgctat tttctgagtt tagctttgta aatgaagaag atatgtacaa
    19261 atcaaaatca ctttttttat aagatgatgg caaagtatgt gacacattaa aagtaatatt
    19321 gggatatagt tttctgaaat tactcaaaag tgatggtaac acattagccg ctgcaggaga
    19381 taatatcttt acttctcctg aattttctaa agaagtgtct tgtaactcct tttttgaact
    19441 ttctactaaa tctaaaatgc tatctactct ttttagaaac aatcttccat actcatttaa
    19501 aacaatatat cttccttttc tgtcaaataa ctgaactcca agttcttttt ctaaggaaga
    19561 tatagttttg cttaaagctg gctgtgctat atttaacttg tgtgatgctt ttgttatgtg
    19621 ctccattcta gcaacagttt gaaaatactt aagatgcaat aaatccatag tataaaatac
    19681 acctctttta atatgacttg agttatatca attttataat caactccaca ttatacatta
    19741 taatataaag tttatataat caactacaat atattaaaaa gttatttaaa ttgttattta
    19801 tattttgtac ataatctaat taaaaaaagt agctttaaat gattaactaa ggaaatgttg
    19861 ttacaaactt tagttatata aaaaattcat ttgatgagta aagtattaat gtcaagtaag
    19921 aataaaagct tattagatag aaaaataata ttttataata atgatgttac ttaaattatt
    19981 atgttatact attttaaata ggaaacattt agatactata attttagggg gagtcaagat
    20041 gaaattaact aaatcaatat catcaatgct atgcttagct ctagttttaa gcttatcacc
    20101 agtagcatat gcaaatgaaa taagtagaac aacagtggag aatactttag taggtcagga
    20161 cagataccaa actgctgttg aagtaagcaa aaaaggatgg agtagtgcga atgaggctgt
    20221 agttgtaaat agtgaatcta ttgtagatgc attagctgta acaccatttg ctaaattaaa
    20281 aaatgctcct atattattga cagaaaaaaa taatctaaat tcacaaactg agaaagaatt
    20341 aaaaagatta ggagttaaaa aagtttatat aattggtcta tctagttctg tatcaaatga
    20401 tgtgcaaaaa cagttagaat ctaataattt aactactgat agagttggtg gtagtgatag
    20461 ataccaaaca gctttgagta tagctaaaaa aatagaagga ttaaaagata tatcagaaat
    20521 agctgtagta aatgggtaca caggtcttgc agatgcagtt agtatagctt ctgtagctgc
    20581 tactaatgga atggttatac ttccagtatc tgatgcatct ggtatatcta gctttaaaga
    20641 ttttatagaa tctaagaata taaataaatc atacataata ggctctacta atgctgtatc
    20701 agataaaata aaacaaagtt tacctaattc agaaagaata ggtggagcag atagaaatga
    20761 aactaatggt aaagtaatag aaaagtttta tacatctaat aaattaaaca atgtatttgt
    20821 ggctaaggat ggaatgaaaa atcaaaatca acttatagac gctctagcag taggagtact
    20881 ggcatcaaaa gaaaattctc ctgtactaat cgtaggagaa caattaagtg caacacaaaa
    20941 gagtgtattt ttgaataaaa atgctacctc tataactcaa gttggtagtg gaggaaatga
    21001 aaatgcattt aaagaattaa aagatttaca ggcaaatagt aacaataaga gagtaatgta
    21061 tgtaactaat acagataagg taaatataag aaatgatgct acaatagaag cttctgttat
    21121 aggatatcta aataatggtg atgaagtaga agttttagat gtgttaaaaa ctggatgggt
    21181 aaaaataaaa tacaatgaag gtattggata tgttagtggg tcttatttga caaataataa
    21241 gcctgataat agtaatgaaa atataaaaat aaagtatgta aaagaaaaag atggtctaaa
    21301 tgttaggaaa ggaccaagta cagaagatga aaaaataggt cacctttcat atggaagcaa
    21361 agtagaaaca atagaaatat ttgctactgg atgggtaaaa ataaagtata atggtggata
    21421 tggttatgta agtaatgatt acttatctga tacaccagtt atttaattat aagtaacaat
    21481 aaaaaactgc tttaatttgt aaagcagttt ttttataaaa atttaagtct aataactatt
    21541 tctattttga taccttaatc ctgggattta ttattatgga tattatcaac atacaaacac
    21601 tccaaatcca gtgtctaatt ccaaacttag atattaataa attcagtcct gctatggtaa
    21661 ataagcagta tttaattaca attaaaagtg tatttataga aatccccttc atagaaagta
    21721 tcctaaatcc aataatagac attctaagtc ctataaatgc caataatata cctgcaatat
    21781 cctgaattaa gtaatagtat tcaaatttca tattcaatca actcttctct gagcagtatc
    21841 aaatatttct aactcttcaa tttcatcatc tgtgtatgta agcatagtaa aaccagttac
    21901 accataaata aatgaaatta taggtgtaat ccaacttaag aaacaatatg gaataaattc
    21961 tagtgggctt acaccaagag tctgagcaca gaaaacagca tttgaattcc atggtattaa
    22021 tggaccacca agtgtagctg tatcttctat aattctagat agattttgtg gttttaattt
    22081 aaactctttg tatattggag acattagagt tccgacaaat acagaagtaa acatcattga
    22141 tgaactaaat gcatttgcaa agtagcttac tataaaagta gacccaacca atttagttct
    22201 tgattttcct attttagtta taagaggttg aagcagtact tctaagaatc cagttttttg
    22261 gagaataccg gcaataacga aaactacaaa tactagtaaa acggtttcag ccatactcat
    22321 tattccacca cgatttaata gtttgtcggc atagacaaat ccagtatcta tagtaaatcc
    22381 tgatagcatg taatttaaaa tatcacctat tttttctcct tcttgaaata ctgctataat
    22441 aagtcccatg atagctgaac ttagtataga tataatgggt ggcttttgta gcaataaaag
    22501 taataataaa aatattattg gaatcattgc tactaaacca atatgaaagt ttgaatttaa
    22561 tacatccttg atttgattta tttgaacata gtctatagta ttatttgaat acttaaatcc
    22621 aattactgta tacagaacta tacatattac gtatgcaggt cctgtggtat aaaacatatg
    22681 cttcatatgt gtaattacat ctgtcttaca aactgctgca gctaagtttg tagaatctga
    22741 tagaggtgac atcttatctc caaagaaagc accagaaatg actgcaccag ctgttaaacc
    22801 aagaggaata cccatactag taccaatact catcattgct ataccagaag ttccaagagt
    22861 cccccaagat gtaccagtag caacagaagt taatgaacat acgattaatg ttgtaagcag
    22921 aaaatattta ggggttatta ttgttagtcc actatatact actgttggta ttgtaccagc
    22981 agctatccaa gctgctatta gtacaccaac agaaagtata attaagtttg attgaaaggc
    23041 atctttacca acttcaaatc caaaagcttc tatttcatca ttagtataac caagtttcgt
    23101 ggctgcagga actactataa gccatgaaag taaaaacatt gtagttatag aagcattaaa
    23161 tacaactata ccaactgatg ttagagctat tattgcaagc attactaaca aggaatatct
    23221 aaatgaaggt atttttcttt ctgtattcat tttcatagtg caaattctcc ttaaatattt
    23281 ttatttaatt gcatttttta ttcttataag agcttcttca agcatacttc ttggacaagc
    23341 taagtttatt ctttgatacc cacttcctcc aatgccaaag ctgtttcctt gatttagagc
    23401 aactttacct ttttgtacaa gtacactttc tagttcctca tcacttaatc caagtgcact
    23461 aaagtctacc cataataaat aagtaccttc tggttttcta actttaagtt ttggcatatt
    23521 ttcatttata tattttatag caaaatctat attagattct aaatattcta aaaatgattc
    23581 tagccaagat tcaccattat tatatgaagc ttcagttgca actaagctaa agcaattatt
    23641 tctctttata tcaattctgg taaaagcatc atctaacaat ttatagtctt tttcatcagg
    23701 tagtattaca tatgatgatt gaagaccagc tatattaaaa gttttagttg gagccataca
    23761 agtaattgta tttttttcaa attctttaga aatagatgcc ataggaatat gcttatgttt
    23821 ttttaatata atgtcactat gtatttcatc agatatgatt tttacattat gcttaagaca
    23881 aatatctcct agttttttta attcatcttt tgtccatact ctaccaactg gattatgagg
    23941 attacaaagt atgaataatt ttacatcttt aatcttgttt tcaatgtctt cataatccat
    24001 tatatagttt ccattttcta gtttctgtaa tgggcttata attagttctc tattgttatt
    24061 ttttacaaca ctattaaaag ggctgtaaac tggttcttgt atcattattt tatcatttgc
    24121 ttttgtgagt tcatttatta aaagacttat agctgggata acacctggac tataaattaa
    24181 ccactcactt tttattttcc agttatgtct cctatctagc cagtttacta tagattcatt
    24241 gtaagaatct ggtcttgttg tgtagccata aatctcttgt tctagtctat tttttaagga
    24301 atcaattata caaggtgcag ccttaaaatc catgtcagcc acccacatag gaagtaaatc
    24361 attagttcca tacttttttt ccatttctga ccatttagat gaaaaattat tgcttctatc
    24421 aacaatctca ttaaaattat aattcattgt aaatacctcc agtaatttta attttatgtt
    24481 aataatatta agcaaacaac gtgccaatat tgataataaa aaatatttat tcacaagtta
    24541 atatttaata ttaagtaatt tcagagcata gataagataa aaatattaat tatacttaaa
    24601 actttgtgga aaaggttatt tctaataaat ttttaaatat atgaaaatgt aattaaatag
    24661 gttaaatagg tttaactgtt taacttgtta gacctattta tattttttag aaattctact
    24721 ccttttgatg ttataattga acctccacga ccaacaagta tgtctacaag cccaaagtct
    24781 tgtagttcta aaagcatatt tctaatctgt tgttctgtca aaaatctatt ttcctctaaa
    24841 gctattttac ataagctttt tctacctatt ctttctttta aatcataagc cttttttaaa
    24901 ttatccaata taaatatgta ttcatctagt gaccttttaa aattatagtc gtatttcatg
    24961 atgtttttgt ctttgccaat gttgaattga tactttttta tattatcact tactttatta
    25021 caaacggttc tactataatt tgaatctata gtgtccaata tatattcagg taaatcacat
    25081 atttctataa tatcttttcc caagtaacag aagtattctc ctaagtttct taattctctt
    25141 acatttcctt cccatctata cattttaaag atttctttta cttcctcact tagaataaaa
    25201 ttacatggaa tatcatattt taatgaacca aaaatttcaa atatatcagc agctctttcc
    25261 ctaagtggat gtattttaag gggaagaaca ttcaacctaa aatataaatc tcttctaaac
    25321 atatttttag atacaagttc ttttaaattt ctattagtag ctgcaattat tcttacatca
    25381 atatcaatga cattattaga accaatacgt cgtacttgtt tttcttgtat gactctaagt
    25441 aatttagatt gagaatttaa atccatttct ccaatttcgt ctaaaaatat agttccattg
    25501 ttggctaatt caaataaacc aattttccca ccttttttag caccagtaaa agccccttca
    25561 tcatagccaa ataattcact ttgcagtaga ttttcttgaa aagtactaca atttactgcg
    25621 acaaagggac catcttttct cctagaagca ctatgaatgg attgagcaaa taattcttta
    25681 ccagtaccac tttcaccaat tataaggata gatgaatttg attgagccat tttattagca
    25741 attttttttg tattaatagt ttgtatacta gaacctaata tatcatcaaa tgtgtattta
    25801 ctgatattac cagagtttac tagttgtgct cttaatttag cttgcttttt ttctaattga
    25861 gaaaactttg tcatctttag tataaatcca tcaaatacat ttaattgtat atgtttaatc
    25921 tctaaattta tatcaatatg atttatttta atcaatttct caaaaaatgg agttttattg
    25981 gtcaatatgt tttgaaaatt gatgtctttt atacaatcac caacaaatga gtcaatcatt
    26041 tcatttgcat ttatagacaa aatttttcta gctatatgat tatagaattg aattattcca
    26101 tcattatttg tgcatataat tccatcatca attatagata gtaacaaatc aaattgtctt
    26161 tcaaacttat ttgcatctaa caataactca cctgtactat aacttgtagg aattattttt
    26221 tctacgtatt tttttattag gtcatctttt attagatgtt ttaatttggt ttttgtagca
    26281 atttcaacaa tacaacttaa atctattatt ctgtatttta aatctacaac ttcacaattt
    26341 ttaaacttag gcaggatttt ttcgccagga gttaggatta cagcatctgt gggagtaaac
    26401 tcaatctctg gatagcaggg caggatattt atattgtcta ttcctaatct atgtattaaa
    26461 gaaattgttt ctattgccat atctttactt aagttatata cataaataat tttatctttt
    26521 tctaatttgg atatcatatc aatactgttc ttttcaaatg tcaacttggg aacaatgatt
    26581 tgagcatcat agtgagataa ttttactatt tcatcgtatt ttaaataagc agataggagt
    26641 ataagttttt ctttaaaaaa ctttaaattt ccctcttcaa atgagtataa atttatcttt
    26701 agattatcac ctaagaattg ccttatttga ttttcataaa tttctccagc gtcttttttt
    26761 aaacttacaa tagctaattc catttgtttc attttaacct ctcatacaaa tttgacttat
    26821 tttagattac ataataatta aattagatta aataatatta tttaattatt attataaatt
    26881 tattatagac tacacattga agtaaaagaa gtagctaaaa ttattccttt tatttttgtt
    26941 ttttgtatct atttggtata atagtattag agcgtataat aatttaggca aaatttttta
    27001 aggaagacta gaggagaata taaaatgaaa aaatgtcttt actgtaatag ggatataaag
    27061 tcaccaaata ataaattagc tataaaatat gatgacaata tgaatatata tccatgtcgt
    27121 gcagaatgta aggaaaaaat tgaagaatat atagcaaaaa aacctacata taagtttata
    27181 aatatattag aggttctttt aggtgtgggc ggaatattct gctttttgag taaatggact
    27241 attgcagccc aagttgtact tggaattgat gcactggtta tatttttatt cccattggca
    27301 ggatttaaga tgaatggaaa gttaagtatg gaacaaacta agaatcaaag taggtctatt
    27361 gcaatatcaa ttttggcatt tatagtaata ataaccgtat tatattttaa acaagttggt
    27421 aagtagagca taattgtata agacatcatt agagtatgag gtgagtttat ggaagaaaga
    27481 aaattagcac caaacaatga gagggaagct ctcataaaaa gattaaaaag aatagaaggt
    27541 caagttaagg ggatacaaaa gatggtggaa caagaacgtt attgtgttga tatattggtt
    27601 caaatatctg ctataaggtc tgctataaat agagtaggca caattatctt agagaatcat
    27661 ataaaaggat gtgtagcaga aagcattaaa catgatgatg ctgataaaac tgaagagatg
    27721 ataaaagagc ttatggatac tataaataaa tttacaaaat aaggaggcgg ttagttatga
    27781 atcaacaagg atttattatg gaaattgttg atgatagaac tgcaaagtta aaaatgcaaa
    27841 gacattcagc ttgtgctgct tgtggaaaat gtgctactac aaattcagca gagtcaaaag
    27901 aaatattagt agaagtagac aacacaattg gtgcaaaagt tggtgaccat gtagaagtga
    27961 gtatggataa tatgaatgta ttaaaagcag ctgtaatggc ttatataata cctcttatag
    28021 cacttcttgt aggaacaata gggacttatt atatattggg ggcaattgga atgacaactg
    28081 gagttgaagc cataagtgga ttagttggaa tttgtcttac aataatctgt tatttatact
    28141 taaagaagaa tgacaaaaaa tttagagaca gtagagaatt tataccagta ataacaagaa
    28201 tattaattga cctttaagat aattataatt aaaaaatagc aatgtgcttt ttaaataaat
    28261 actcaatacg tatttgtaga gtttaagtac attgctattt ttatattttt attttgattt
    28321 tctgtaagga ataaagtttt ttctgatttt caaaagattc aatgttctta caaaagaagt
    28381 tacaactaat acaccaagtg ctattgcagc acttagaaat agcgcatctg taccttttga
    28441 catagccaaa gaataatttt tctgaatcaa agacaacatt gtattatata gcccaattcc
    28501 tggtattaga ggaagaatac ctggaattac gaacacaata gctggttgct ttagttttct
    28561 tgctaaagtc tcactacacg ctgagaccaa agcagcagca ataaaacctg caaaaactgc
    28621 atttgtagta aaattaaata aataaacata tacaatccaa ccaattccgc cagttactcc
    28681 agctggaaga agtagatata gtggagcatt aaagaaaaca gcaaatccag cagttgcaac
    28741 acctgaaaat aaaaaatgca aaatcataga catatcagtc attagaaagg acctcctatt
    28801 ctataccatg tatcaaggac aaaaccaact ccagaagcaa ttgcaataat aatcatagca
    28861 gcatcaaaag cacgagaaat tcctgatatt aaattccctg atattaaatc tctaatagct
    28921 tttataaaag caactcctgg gagtagtggc attatagcgc cgactataag cattttaggg
    28981 tcatctaaaa tgcctatttc aactagtaga actcctgaaa gtgcaattaa aaaagaagat
    29041 actaaacagg aaaatgcaga tattgcactt atcttcatcg ttttatcata agtaattgca
    29101 gcaaagattg ctattataaa cgtaagtata aaatttaata cataattccc tccaagcaaa
    29161 caagcaaaac aagcagatgc aattcctgta caagagtaaa ataaccataa aggataaggt
    29221 tttacatctt ggatttcata aagacgtgca atggcattat caatatcata ctctttgtca
    29281 ctaacaaatt ctcgagaaaa actgtttaat aaagaaatct tgtttaagtt tatacctctt
    29341 gattgaatcg ttttcataaa cgaaaagcca tcaaatttct catctgaaat aattattact
    29401 gtaggagttg taaaaatatt tacatgataa aaccctcttg acctgcatat tctaaggaca
    29461 ctgtcttcaa ccctagatgt ttcagctcca ttttttatca tcagttcccc tacaaataga
    29521 gctagtttta gaatattttt cttatattca agttcctcat tttgattcat aaatacacct
    29581 ccaaattaaa aaacgtaata ttgaaattat atcaaatttt taagtaatat tccttaatat
    29641 ctatataaat atattatctt ttttttgttt caaaatagtc acatttaaat tatatccaat
    29701 taaaagaatt agacaatatt atttattata ttttaactta aaaataaaga aagtaactat
    29761 gttaaatatg gaaaaatggc aaaacacttg actattaatt ggtaaaggac tataataata
    29821 aattatcata ttgataaaaa cgaaaattat gaaatgtctg taaaaataaa atttaaataa
    29881 taactaggag gagtaatatg tttgagaact tgaaaaaagc ggatcctgag atttatgaaa
    29941 gtatgaaaag agagttaaaa agacaacaaa gaaatattga attaatagca tctgaaaaca
    30001 tagtatcagt tccagtaatg gaaactatgg gaagtcatct tacaaataag tatgcagaag
    30061 gttactcagg caaaagatat tatggtggat gtgaatacat agatgaagtt gaaactcttg
    30121 caatagatag aatcaaaaaa atatttggag cagaacatgc taatgttcaa cctcactctg
    30181 gagcaaatgc aaatattggt gtatattttg ctatgttaaa accaggtgat gttgttatgg
    30241 gaatgaattt atctcaaggt ggacatttaa ctcatggtgc tccagttaat atttctggtc
    30301 aatattataa attttatgaa tatggtatag ataaagacag tggacttata gattttgatg
    30361 aagttagaaa gatagcacat gaagtaaaac ctaaaatgat agttgcagga gcaagtgcat
    30421 atcctagaga aatagatttt aaaaagttta gagagatagc tgatgaggtt ggagcattgc
    30481 ttatggtaga tatggctcat atagcaggtc ttgtggctgc tggtattcac caaaatccat
    30541 gtgaagtggc tgattttgtt acaacaacaa ctcacaaaac tttaagagga cctcgtgggg
    30601 gtgttattct ttgtaaagaa aaatatgcta aagacataga caaagcaata ttccctggta
    30661 ttcaaggtgg acctcttgaa catataattg cttctaaggc agtttgcttt aaagaagctt
    30721 taagtgatga gtttaaagaa tatcaggtac aagttgctaa aaatgcaaaa gccttagctg
    30781 aagaacttat taaaagagat tttaaattga tttctggagg tacagataat catttaatac
    30841 tattagattt gattaataaa aatataactg gtaaagcagc agaaaagaga ttggatgatg
    30901 cttatataac agctaataaa aatacaattc catttgatcc aaatggtgct cttgttacaa
    30961 gtggaataag acttggtact ccagctgtaa ctactagagg tatgaaagaa gaagatatgg
    31021 caataatcgc tgaggctata gacctatgtt taacttatga tgaagaatct aaggctagaa
    31081 cattggttgt tggtttaact gaaaaatatc ctttatatga agaatataat attatggatt
    31141 aattataaag aacaactaaa taaaatacat ttaaataatt acaatgattg tgtatgtatt
    31201 tgaatatatt tagatataaa aaaggtgtct caaaatgaac tttttagttt atgaggcact
    31261 tttttgtatg gaatcgaaca tatttgtgtt atttaaataa taaaaaatat ggttatctct
    31321 atttttagat ggtctctttg tattttttaa ttaaaacttt aagatttctt aagaattata
    31381 ctaggttttt ctaaagattt aaagtctata cttataagta tagtaatcat atgaaaagaa
    31441 aagctttaac gaggagggaa tcttatgaag gtattaattc ttacaggaaa atttggtatg
    31501 ggtcattact cagcatcaaa ttctttaagt gaagatataa aagcaaaatt tgataattca
    31561 gaaataacaa ttaaagatat atttgaatac attatgccta actattcaga taaaatgtac
    31621 aagacttttt ctattcttgt aaatcgggga agtagtcttt ataatttgtt ttataaatgt
    31681 gctgaaaatg gaaagaaaga tataaaattt actttttcag attatttttt aaataaatta
    31741 gatacattac ttcatgaagt acaaccaaca gtagttattt caacttttcc attttgttct
    31801 caattagtat caagatataa ggaaaaatat aactcaaatc ttcctttaat aacatgcata
    31861 actgatataa gtagtcactc agaatggatt agtaaaaata cagactgtta tttagtagct
    31921 agtaaatcta ctaaggaaga acttgttttt aaaggaatag atgaatctaa gattaaagtt
    31981 aatggtattc cagtaaaaaa agaatttaaa agaatagaac atgtaaatca ttcaactaaa
    32041 aaaaatattc ttataatggg tggaggatta ggattactgc ctaaatcaga acaattttac
    32101 aaagaattaa atagtttaga aggagtaaaa actactgtaa taactggtaa taataaaaaa
    32161 atgtactata aactatatgg tagatatgaa aatatagagg tagtaggtta caccaatgaa
    32221 gtatataaat atatgaaaga ttctgacctt attatttcta aaccaggtgg aataacacta
    32281 tttgaaacaa tttattcaga acttccaata ttggcattta atccattttt acaacaagag
    32341 atagataatg ctagttttat attaaataat gaaattggga gaatacttgg taaaaataaa
    32401 aaatactatg tagatgagat aaaagattta atttatgatg atgctactct taaagaaatg
    32461 agcagtaaca tgaaagaatt aaaaaaacaa tttgacaata ataccttaga gaatatttta
    32521 ttttcacttg atgaacaagg agcgtgtaga gaatgtatgt agtaggttta attatcgttg
    32581 ttgcattgat ttttttagtc cattcaatta tacctacata ctataataaa ctattaaata
    32641 aagaggtatt aaaaaatatg gctggagaaa atgaaattgc acttacattt gatgatggtc
    32701 cagataagag atatacagaa aaattactag atgtgttaaa agaaaatgat atacaagcta
    32761 tgttttttgt agtagcaaag aatgctgaaa aagagcctga aattataaaa agaatgttga
    32821 gagaaaatca tatagttggt ttacattcat tggaacacag aaatgcatgg ctttatagtt
    32881 atagttatgt taaaaaagat tttattgaaa gtacaaatat aatgaaaaat ttaggtgtag
    32941 atgtaaacta ctatcgtcca ccatggggtc atactaatat attttcaaat tcatttgtta
    33001 aaaaatataa tctaaaaatg actttgtggg atgtaatggc agaagattgg gagaaagata
    33061 gtacagtaga cattattata aacaagttaa tgagtagaac aaaagaaaat tctataattt
    33121 gtttacatga tgcaggtgaa aattcaggtg gtgcagtagg tgcacctgag cgtactattg
    33181 aagctttaaa gatagctatt ccaaaactta aagccagtgg tttaaagttc gtaacaccag
    33241 aaaggatgta gttatgataa gtaaaactga aaagaaaaat aaatgcctag gtagtgtagc
    33301 ttttttagtt ttactaatgg gcataacagg atattttgtt tttagagggc agtcagttga
    33361 atcattgata aaatcattaa aaggtgcaag ccctatgttt atactaattg gatttgcaat
    33421 gatgtttata tatgttgcct gtgaaggtat aaatatatac ttaggtatga aagctttaaa
    33481 ccaaaaaact actcttttaa aatgtatggg atatgctttt attggatttt attttagttc
    33541 tattacgcct tcagcatcag gtggtcaacc agcacaagtt tattatatga aaaaagacga
    33601 tataaatata tcatattctt cacttatact attagtcatt gttgttattc accaagtagt
    33661 tatactggca tatagtggaa taatgttcat tatggaacgt gagtttatat taaataatgt
    33721 aagtggtatg aacattttac taatatatgg tgtaataaca aatgttgctc ttgtcattgg
    33781 agtaattgca attatatttt caaaaaaact tgtaaacaat tttataatat caataacaaa
    33841 tttattaggt aagttaagaa taataaaaga tgttgaaagt tcaagaaaag ttataatatc
    33901 tcaaattgaa gagtatgtta aaggtgctca atatataaag cagaatccaa aacttgtagt
    33961 ccaaatttta gtcataacta ttgtacaaat aacagctatg tttttagttc ctttctttgt
    34021 atacaaagct tttcatctat caacatatac tgtatttgag atattagcga tacaatcctt
    34081 gcttaatatt gctgtttctt cattaccatt gccaggggct gttggagctt ctgaaaatag
    34141 ttttatgaca ttatttaaga tatttttccc tggtcactta ttagttccgg caatgttact
    34201 tagtagggga ataagttttt atgcatttgt agctataagt ggattgatat gtatagtagt
    34261 acatgtaaaa tcttctgaaa aagtaaaagc agtgaaaaaa gttgcttatg taagataata
    34321 ttatatagat tatttttatt aatatgtcaa tttgtgtgta cattttaatt aagttttaaa
    34381 catgtttaca ggagtataaa ctattaaaac gtgtgacatt gctaagcttt tgctaataaa
    34441 gtataatatt ttatatagtt tgaaatattt aagaaatata gcaaaaaaag gagcttggtg
    34501 atgaataaag agattccaaa aaaaatttta gtagctgatg ataatcctga gattagggaa
    34561 atcgtagaaa tacttttaac tggtgaaggt tatgaagtta ttatggcaac gaatggagaa
    34621 gaagctgtaa acttagtaga tagtacgatt gatttgatta ttttggatgt tgtaatgcct
    34681 gtaaaaacag gatttgttgc atgtaatgaa attagagaaa aaacgacagc accaatatta
    34741 tttttaactg caaaaactca agactctgat aaagttattg gattttcagc aggaggagat
    34801 gactatttat caaagccttt ttcatattct gaactaattt caagggttaa gtcgctactt
    34861 agacgttatt atatatacca gggaaaggaa aaggaaagag agccaaatat attaaaaaca
    34921 aaagagttat gtgtcaattt agatactcaa gatgttacac taaatggaga acctgtgttt
    34981 ttgacaacta tagaatatag tatattaatt cttatgttga aaaatagaaa aaaagttttt
    35041 tcgtctgaaa atttatatga aactatttgg gatgagcagt atttttatac ggcaaataat
    35101 acaataatgg ttcatattag aaatttaaga aaaaagttag aaaaagacca aaaaaatcca
    35161 caatatataa aaaccgcatg gggaaagggg tattatattg attaacaaaa ttaagccatc
    35221 taagttggga gttatactat tgatatatgt agtaatctca gctgcagtgg ctttcatttt
    35281 aaatatacca ttttactctg ctgttgaatc tatagtaaaa gatgtatcag aagggaaagt
    35341 atattttaaa agagaagtaa atcaacttat ggataatttt caaacttatg tgaataacaa
    35401 tggtataaga gcagatgatg gagatgatat agcaaaatgg aatagtgata attggtttgt
    35461 ttctatgaag atatataaag gaaataaggt tttttatgat acattatact atcctaatga
    35521 agtacctgat aagagtgagg aaaatgagtt ttatagcagt cccatggcta gaaactatga
    35581 gatacaattt tctgatatta aagctaatat agttgtagta gctttattta gatttagatt
    35641 tcaaaatttt atagaagcaa tttctattac atttatggca cttacattta ttataacatt
    35701 tttactgttg tttagtagaa agataaagta cctaaggaaa atagaaatgg gtataaagat
    35761 agtagagagt ggcagtttaa agtatagaat cccagtaaaa ggtaatgatg aattatcatc
    35821 acttgcatct agtattaatg agatgagtca tacattggag aatcaactta taaaagaaag
    35881 ggagatgaag aataaaaata aagatatagt aacttcgata tcacatgatg ttagaactcc
    35941 attaacatca gtaatttgtt atttagattt gatcaaagac cataaataca atagtcctga
    36001 gcaattggaa caatacataa aaaatgtaag gtctaaagca tatcaaataa aagatttaac
    36061 agatgacctt tttacgcatt ttgttaattc tggagaggaa gtttcaaaag ggtatgaaaa
    36121 aataaatgct aatgaattta tttttcaatt acttttagat agttcatata cattggagga
    36181 gaaaggattt catataacaa tagaaaatga actttataac caatatcata tttatgtaga
    36241 tgttacacaa tttagaagga tatttgataa tttatgttct aatataataa aatatgctag
    36301 gaaagataag cctatagtat ttttaattaa agtagaggat tctaaattac taattataca
    36361 aaaaaataga gtaagtaatt tttctaagaa tgtagaaagt tttggaattg gtataaagaa
    36421 ttgtgagaaa atagttgcat atcataatgg aagcataaat gtaaattcag atgataaaga
    36481 atttgctatt gaaatatcta ttccagcaaa agaatcataa aaatttaaat tatatattgt
    36541 gtaattgcta ttatatattg tgtaattgct tatgtaaaat tctatgaagc aggaagcatg
    36601 tgactttagt tatgggatgt tcaatattgt gtacctctag gcaagtaaca agtttactta
    36661 caagaccata taatattatt atcttaaaaa aaggggacat aataatatgg ttttgacagg
    36721 agcacattat atttacattc tatttatggt aattatatta attactatga ttatgaaaaa
    36781 ggatacaata gtaccatgta tattaggtgt tttttttatg ggattgttct ttgaaaaaaa
    36841 catctttggt gctataacag cagtgtttaa ctcttttatt atatctttaa atgagttggg
    36901 tccaattatc ttgattatag ctattatggt tgcattatct aaagccctag aagccaataa
    36961 tgctatacag tatatggtga gaccattttc gagagttata aaaaattcta atacagcatt
    37021 ttttgtaact ggatttgtta tgttagttct ttcttggttt ttctggccaa ctccagctgt
    37081 tgccttagtt ggagctgtat ttttacctgt tgctatgaga gctgggctac cagcaattgg
    37141 tgttgcagta gcattaaatt tatttggtca tggtcttgcc ttgtctacag actttgttat
    37201 tcaaggagct ccaagtataa cagctggtgc agcaggtgtt gcagtatcat atgttataaa
    37261 tgatggtatg attttgtttt gggtgatggg aatcgtaacc atatctatgg cattttatac
    37321 attaaaaaga gatattaaca aaggaatgtt tagagaagaa ctaaaaggtt ttgaaagtga
    37381 agaagtagaa gagttcaatg gaaaatctaa aatagcaact attttagtag ctctgggatt
    37441 tcttgctgat ataattgtta tgtatatatt tgacctaaaa ggaggagatg catcagctct
    37501 tttgggtgga acagcagtat ttttaataat aattataaat gttataaatt ttggcaaaga
    37561 tagtcttgaa aatgtatgtg agaatataat tgatggtttt gtgtttggga taaaaatatt
    37621 tggagcaatt ataccaatag cagcattttt ctatatgggt gaagttgcgc ccttgacagg
    37681 tgtatttgga aaagtattag caccaggttc acaaggttta ttatcagata taggcatagc
    37741 tctttctcaa acaataccac ttaataaatt tgccgtatca ggtatagaaa ctgtagtggg
    37801 ggcaataact gggcttgatg gttctggatt ttctgggatt tcattagttg gttcacttgc
    37861 ctctgtattt ggaactgcta taaatgcaag tgttggtgca ttagctgcat taggacaaat
    37921 cagtggtata tgggtaggtg gaggctgtct agttccttgg ggtcttattt cagcagcggc
    37981 aatttgtggg gttagtccaa tagagttggc aaagcgaaac tttgtcccag ttataaccgg
    38041 attggttgta acaaccatag tagcaatgtt tattatttaa taaatcgaat aaatctttaa
    38101 ttgtacaaat taaaaataga tgtgtagcta taatttatta taattgtttt actaatttta
    38161 agccatacat ctatttttat tgatattttt attagtattc tttaataaaa taatattttt
    38221 cattatacta tacataacat ctaaatataa catctaaaat actgttatct atgtttaata
    38281 tcttgccaaa ttttttgatg ttttattacc ctgtcccacc aacatatata agctatcgca
    38341 agttttttac ctatactatc accaaacaaa aattttaatt ttaatttatt attttataat
    38401 ctatattata ttaactatat tagtttgtag actttagatg taattctaaa tattaatact
    38461 ccaagagttc aaattcatat cctagttttc ttgcatttga tataaaatca tttaaaattt
    38521 ctgtactagt tttggaaact gcatgtagta acaagataga accatcatgt agattatcca
    38581 ttattttttg cgttgcttct tcatgtgatg gttgtttatc agtatcccag tctctgtatg
    38641 caaaactcca aaatacagtc ttgtatccta aatttttagt cattgccaag cttttttctg
    38701 agtattttcc cataggaggt ctaaaaaatt taaccatatc tttcccagtt acatctttat
    38761 ataacttttc aacatcatat aattcgtcat taaagttttt tagattgctt gtttttgttg
    38821 gcatagatgg atgggtttta ctatgatttc caactatatg tccctcagaa accattcttt
    38881 ttactaaatc tttattttct tttatatatg gagctgtaac aaaaaaaact gctttaacct
    38941 ggttttgttt tagagtgtcg agtatctttg tagtgtatcc attttcataa ccttcatcaa
    39001 atgtaagata tagtgttttt tgaccatctt ttgtaggtcc attgtatagg gcatcatagt
    39061 cagaaaattt aaattcaatg tccgtattta cttctggtgt tttatgctta ttgttaggta
    39121 tataaaacca atcaagagta gtgttatcta aagaagatac atctaattta ttattatcat
    39181 tattagtagt ggcttttgat ggtttatctg ttccgtctga aactattaca ttggaagtat
    39241 ccttaccacc atctattttt tccttatctt cttgtaactg tgtttcctta ttttgatttt
    39301 cgttttgatt gttttgtgaa tttgaacatc caacaataaa aaaaatagaa atagaaagta
    39361 gtgttaaata atgcaatact tttctaaaca ttttgaagct cctttctaaa ttaataataa
    39421 ttgcatatag tattattata attggaaatt actattaata atagttatat ctttaaaaat
    39481 aaagtcacaa tagtatgaca aagggtgaat aatcaataaa tttaaaaaaa aatgaaaaaa
    39541 atagttgacc atattatgag aagatgttat tataataaat gtgctcaaca agaagtaaca
    39601 aaataacaag cacaaagaag ttaattgaat
//
LOCUS       DAMKPG010000028        38315 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMKPG010000028 DAMKPG010000000
VERSION     DAMKPG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.030"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            27..233
                     /locus_tag="PC621_001554"
     CDS             27..233
                     /locus_tag="PC621_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108716.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
     gene            complement(544..1287)
                     /locus_tag="PC621_001555"
     CDS             complement(544..1287)
                     /locus_tag="PC621_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF4108717.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1453..4020
                     /locus_tag="PC621_001556"
     CDS             1453..4020
                     /locus_tag="PC621_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108718.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNNSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAVDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4250..4654
                     /locus_tag="PC621_001557"
     CDS             4250..4654
                     /locus_tag="PC621_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4108719.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4678..5175
                     /locus_tag="PC621_001558"
     CDS             4678..5175
                     /locus_tag="PC621_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108720.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5178..5579
                     /locus_tag="PC621_001559"
     CDS             5178..5579
                     /locus_tag="PC621_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF4108721.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5597..6085
                     /locus_tag="PC621_001560"
     CDS             5597..6085
                     /locus_tag="PC621_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108722.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6112..6867
                     /locus_tag="PC621_001561"
     CDS             6112..6867
                     /locus_tag="PC621_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4108723.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6867..7730
                     /locus_tag="PC621_001562"
     CDS             6867..7730
                     /locus_tag="PC621_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF4108724.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7777..8256
                     /locus_tag="PC621_001563"
     CDS             7777..8256
                     /locus_tag="PC621_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF4108725.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8321..9628
                     /locus_tag="PC621_001564"
     CDS             8321..9628
                     /locus_tag="PC621_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4108726.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9939..10079
                     /locus_tag="PC621_001565"
     CDS             9939..10079
                     /locus_tag="PC621_001565"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF4108727.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10591..11685
                     /locus_tag="PC621_001566"
     CDS             10591..11685
                     /locus_tag="PC621_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108728.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11690..11890
                     /locus_tag="PC621_001567"
     CDS             11690..11890
                     /locus_tag="PC621_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF4108729.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11890..12807
                     /locus_tag="PC621_001568"
     CDS             11890..12807
                     /locus_tag="PC621_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108730.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12804..13562
                     /locus_tag="PC621_001569"
     CDS             12804..13562
                     /locus_tag="PC621_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108731.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13945..15018
                     /locus_tag="PC621_001570"
     CDS             13945..15018
                     /locus_tag="PC621_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF4108732.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            15088..15351
                     /locus_tag="PC621_001571"
     CDS             15088..15351
                     /locus_tag="PC621_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108733.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15557..16534
                     /gene="bioB"
                     /locus_tag="PC621_001572"
     CDS             15557..16534
                     /gene="bioB"
                     /locus_tag="PC621_001572"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF4108734.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16762..17775
                     /locus_tag="PC621_001573"
     CDS             16762..17775
                     /locus_tag="PC621_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF4108735.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17817..18707
                     /gene="rbsK"
                     /locus_tag="PC621_001574"
     CDS             17817..18707
                     /gene="rbsK"
                     /locus_tag="PC621_001574"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF4108736.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18755..19717
                     /locus_tag="PC621_001575"
     CDS             18755..19717
                     /locus_tag="PC621_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF4108737.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19771..21282
                     /locus_tag="PC621_001576"
     CDS             19771..21282
                     /locus_tag="PC621_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF4108738.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            21266..22264
                     /locus_tag="PC621_001577"
     CDS             21266..22264
                     /locus_tag="PC621_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF4108739.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22448..23710
                     /locus_tag="PC621_001578"
     CDS             22448..23710
                     /locus_tag="PC621_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4108740.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23730..24473
                     /locus_tag="PC621_001579"
     CDS             23730..24473
                     /locus_tag="PC621_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF4108741.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24646..26031
                     /locus_tag="PC621_001580"
     CDS             24646..26031
                     /locus_tag="PC621_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4108742.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(26368..27012)
                     /locus_tag="PC621_001581"
     CDS             complement(26368..27012)
                     /locus_tag="PC621_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4108743.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            27276..27890
                     /locus_tag="PC621_001582"
     CDS             27276..27890
                     /locus_tag="PC621_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDF4108744.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            28111..28251
                     /locus_tag="PC621_001583"
     CDS             28111..28251
                     /locus_tag="PC621_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108745.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(28481..28928)
                     /locus_tag="PC621_001584"
                     /pseudo
     CDS             complement(28481..28928)
                     /locus_tag="PC621_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29698..30537
                     /locus_tag="PC621_001585"
     CDS             29698..30537
                     /locus_tag="PC621_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108746.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            30527..30970
                     /locus_tag="PC621_001586"
     CDS             30527..30970
                     /locus_tag="PC621_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108747.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30981..31472
                     /locus_tag="PC621_001587"
     CDS             30981..31472
                     /locus_tag="PC621_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108748.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(31674..32402)
                     /locus_tag="PC621_001588"
     CDS             complement(31674..32402)
                     /locus_tag="PC621_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF4108749.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            32527..33615
                     /locus_tag="PC621_001589"
     CDS             32527..33615
                     /locus_tag="PC621_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108750.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            complement(34053..34901)
                     /locus_tag="PC621_001590"
     CDS             complement(34053..34901)
                     /locus_tag="PC621_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108751.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            35100..35465
                     /locus_tag="PC621_001591"
     CDS             35100..35465
                     /locus_tag="PC621_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4108752.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            35488..37875
                     /gene="cadA"
                     /locus_tag="PC621_001592"
     CDS             35488..37875
                     /gene="cadA"
                     /locus_tag="PC621_001592"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF4108753.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
BASE COUNT        14933 a         3977 c         6870 g        12535 t
ORIGIN      
        1 tataatttta aaaggagtga tataatatga gtaaatatag ttctttatgg gaatatgtta
       61 agaaaaataa tagtcaatcg tttaaattaa cttttgaaga aataaaagat attgctggaa
      121 tagagataga ccattcgttt ttgaaatata aaaaagaatt aaatgaatat ggatatcaag
      181 taggaaaaat ctctttaaaa gagaagactg ttattttcaa taaaattgat taagatataa
      241 ataattacat ataataaaat aatttgtatt gacgattatt acaaataaaa ataaagtaaa
      301 tcttatataa gatttacttt atttttagat gatatttttt tagctttata ctataaggta
      361 tataaaacta agcctattaa aaatgttata atttctatta ccatcataga ccatatcaat
      421 ccatcaagtt cataaaagta attaagcttt attaaatagc ttctttatta taatttcttt
      481 atttcatata atataaatag tattaaaatt tttatgggag ttaaagtaag catgtattat
      541 tacttagata tttgtataat caacatctag taatttacct ttttggtgaa aatgattaat
      601 aagtttttta ggataattca ttattaattc atctggaaaa ttaatttcct ttaagatatt
      661 ctcagaatac tcatagttac ctatgtcaaa acaaatgtga gcatctgaag ttagtatgat
      721 tttagcacca tattttttgc aaagtgtagc aatatactta cagttatccg agctaccatt
      781 tcttgacacg cctttaatag aacaattatt tatttctatt aagatattct tttctatagc
      841 taatttaatg attaattcat agtcgattgg gtagtttggg tttccaaggt gtcctaatat
      901 ttcaatatta tcatgcttat ttattgcatt tattaaagct ttagtattat tctctaatgt
      961 atttggagaa aaaacagctt cgtgaaatga caagataaga taatctaacc gaggaattac
     1021 aaaaggctct aggtctatgt ttcctttaat atctagtata tttgcttcac agcctcttaa
     1081 aattataatg ttatttatga ttcttgggat attttgaatg ttgtgaaagt accatctgtg
     1141 gggagaacca ggcatcttag gaccatgttc agatgtacct agtatcttaa ttccattttc
     1201 tttacaataa ttaatatttt ctagtaaagt gctatagcca tgaccactga ctatagagtg
     1261 ggtgtgtaaa tctgataaaa aattcatata aattcctcct ctataaaaga tgagggtaac
     1321 gtaatttata ttataaaacg aataggaaag aaaatcaata gttacataat tagacaaaaa
     1381 tattaaaata ttgtagaatt aagcttaata ataaatgttt aacagtatta ctgcttatag
     1441 gggaggttaa ctatgacaca aaagaagcta tcaaggaaag aaaaagtttt aatttgcttg
     1501 aaaaggttaa cagaagaaca cttgaacttt aataatggca ttagatttgg atttgatgca
     1561 gaatatatag ctactgaagt aggtataaat agaagtaatg ctagtaagga attaaatcaa
     1621 ttaataaaag aaagtattat tataaagata aagggtaagc ctgttcagta cttatataaa
     1681 gaaagtattg agagtatttt gggaaagaaa ctaaataaag ggatatttga taatattaac
     1741 tctataaacg aatatagtga aaatgattct atcaaagatg agtatatttt agagagtata
     1801 aaaaaagaga acaatatttt taatttagta ggatgtgatg gaagtttaag gtcccaaata
     1861 gaacaggcta aagcagcagt aatatatcca cctaaggggt tgagtacatt aataatagga
     1921 cctacaggag ttggtaaatc tatatttgca gaatatatgt ataagtatag tttaactata
     1981 aaagaagata gtgataatgc accatttatc atatttaact gtgctgatta ttctgataat
     2041 cagcaattat tattagcgca gctttttgga tatgtaaaag gagcatttac tggagctgat
     2101 aaagataagt atggtattgt acatgaagct aataatggag tattgttttt agatgaagtt
     2161 catagattat ctgcagaggg acaagaaatg ctatttctgc ttatggataa agggatatat
     2221 agaaggttag gagaggctaa taaggtacac gagtgtagag ttatgataat tgcagccact
     2281 acagaagacc cagaaacagc aatgttggaa acgtttttaa gaagaatacc tgtaactata
     2341 aagattccgt ctttagagga aagaggcttt caagagagaa tgaagcttat atgttatttt
     2401 tttaaagaag agagtgttag gattggtgtt aagttaaagg tttcaaaaga agttattaag
     2461 gcatttattt tatataagtg taagggaaac ataggccaat taaaaagcga tatacaatta
     2521 atttgtgcaa gagctttttt agattatatg acctataaaa gggaatgtgt atatgtaagg
     2581 ctctctcttc taccaaataa tattagagaa tcattatatg gaaataatag aaaagaggag
     2641 tttgtacaaa atttcagttt tattggaaaa gatgatatta tttttttacc agaagaaaag
     2701 gattttgaaa gtgaaaattt attaatagaa aataaaaaat atgatttaga tttttatgga
     2761 atgataaaag aaacttggaa taaacttcaa caagaaggta ttaaagagtc acaaattaga
     2821 agtgttatag atggagacat acagaagtat tcctataatt taatgaatac ttttatatat
     2881 aataatagta atcaaacagc ttataacaac attgttaatg attcaattgc aactactgta
     2941 aaatatattt tacaaaagca tgataaatgg agcaaaagag aaggattaga taaattaata
     3001 aaagctatag ccttacatat tcagaattta attgaaagaa taaagattgg aaatatagta
     3061 aagcatccta ataaagatga aattatgaag gaaagagcat atgaatataa catagcaaaa
     3121 gaaatattat ctaacatgtc attaatgtat ggagtagagt ttccagaaga tgaagcaatt
     3181 tttttagcaa catttcttta tttatcgtat gtgggattaa atgaggagag tatagcaata
     3241 ttagtcataa tgcatggtga atctacagca tctagcatgg tgaatgtagc caatacttta
     3301 ctggattgta accatgcagt ggcaattaat atgggattag aagatagagt tcaggatatt
     3361 ttagtacaag ctgtagaaat agccaataaa attgataaag gaaagggaat tttaatttta
     3421 actgatatgg ggtctatttt aacatttgct aaagttattc gtgatgctac aggaaaagaa
     3481 gtaaaagcag tagatatggt aagtacacct attgtaatag aagcaactag aaaggctcta
     3541 actccagaaa tgacattaga gaaattatat tttaatatta tagaatctat aaagaaacat
     3601 tatggagata tggaagttac atatgctgaa aaagataatg gtggtagata ttttgataga
     3661 ctactaattg ataatattag taaaactttg acttttttaa atggggaaaa ggcttatttt
     3721 atattaaaag aagtaattaa tagaattagt gaacactatt ctttagtaat tcaagatgaa
     3781 cttttagtaa agtttatatt tcattgttca tgtatgattg aaagagtaat aataaaagag
     3841 tctttagttt ataaatcata tgaagaaaga attagtagaa ataaagaatt atatttagtt
     3901 ataaaagaaa gttttaagct agtagaagag acattcgata taattatttc agatatggaa
     3961 tatgcaaata tattagatat atttgaatca caatatgata cagataaatt caaaagttga
     4021 tacaaaattt aaaaatagcc aaaaggctat ctttttttta ctttttttga tacagatttg
     4081 atacaggttg aaaaaatctt ttgatacata tcgaaataat aagtttttaa aaaaaattga
     4141 gtaaaaagtt ttgattaaat cctgtaaaat agccatttat tattgataca cataagttgg
     4201 cacggcaatt gcttatataa aaagtgacaa gaaaagtgag gtgaaattgg tgataggaat
     4261 aatagttatg actcatggga gttttagtga agaaattata aaaagctgtg aacttattgc
     4321 aggaccagca gaaagaacag cagcaattaa actaaatagg aatgataata ttgaagactt
     4381 aaataaaaat tttgtagaaa agttaaatga attagatgaa ggtgacggag ttttagtatt
     4441 agctgaccta ttaggaggtt cgccaagtaa tgttgcaagt ttaaacctta aaaaaggtgg
     4501 caagtttcat gcacttactg gagtaaatct tccaatgcta cttgaagctt taattaatag
     4561 agagggaaaa agtttagaag agttagcaga agcatgtatt gaagcaggac aaactggtat
     4621 aaacaatatt aataaagttc ttagctcaat gtaatagaaa aaggagtttg attaaaaatg
     4681 tctttagtaa aatattgcag atgtgatgat agattaatac atggtcaagt gatatataaa
     4741 tgggttaagc atctaggtgt aaaaaagata gtagtagtag atgatgaaac aaccaatgat
     4801 gttattgcta aaggtttgat aaaaatggca gcaccaaaga atattgattt atcaatatta
     4861 actgtaagtg aatcaaggag atatttttat aataatcaag ctgatgataa cgtttttgta
     4921 ttaattaaaa atttagatac tgcaaataga atgatagaag aagggattaa tatcaaaaaa
     4981 cttattgttg gaagaatacc tacaggaata ggaaaaaaga aaatatctca aaatgtatac
     5041 attaataaaa aagagtttct tttaatagat gaatttatta aaaagaatat taatgtatca
     5101 attcaaatgg tacctgatga agaagaagta gaattaaacc aaaatattaa tcgctataaa
     5161 ggagagttta tttagcaatg aataaatata aggtaattat actgtgccat ggaacttggg
     5221 gaaaagtctt agtagaagaa acaaataaaa actttggatt gtcaaatcaa tatgaagttt
     5281 tatcactaag ttctgaaaaa gatgtttctg tatttatgaa agaggttgaa gaagtagtag
     5341 ataaagaaga tgtgaaaatt ataatatcag atttatatgg aggatctaca tcaagtgttg
     5401 caatagctgt tgctataaga agaggaatag atgcaataaa tggacttagc ttacagacaa
     5461 ttttaatcgt agatgaagaa ttaagcaaag aagataaatt acatacatta ccagaaagaa
     5521 tcgtcaaaaa aaataataac ctttgtgtgg atttaattaa agaatttaaa aaaatataaa
     5581 ttatgaggtg aatataatgg caaaaataac attagtaaga gtagaccaca gattaattca
     5641 tgggcaagtt ataacaaaat gggtaaaaat cgctcaagct caaaaaataa taatagttga
     5701 tgacttctta ggtcaagatg aatttatggc tgacatatat aaaatggcag caccatcagg
     5761 agtagaagta gttattttaa cagcagaaga tgcgggacaa gcattccaaa acaacacttt
     5821 aggagataaa aatatattta ttctatttaa aaatgttgat atggcaaata aggcttataa
     5881 agctggactt aaatatgaaa agattcaatt aggaggtatt ccaaatgaag caggaaagaa
     5941 aatggtgttt acagctgttt cattaggaaa tgaggatgta gaacaactta atgaacttaa
     6001 tgaaaatggg gttgaaatag tcctacaagt tataccagag gaatcatcta tgacatatga
     6061 aaatgcgtta aagaaattta aataaaaaat ataaattagg aggaattata aatggaatct
     6121 gttttgatgt tggcaattgt aacaggatta tggtattggt ttgcagcagg tcttgcaggg
     6181 tatacccttt tctcaacact aaagtctcct ttatttatag gttttagcct tggattactt
     6241 tggggtgatg taacaactgg tatgattgtt ggtgcaagta tagaaatggt ttatctagga
     6301 atggtcgcag cagggggaaa tattccatca gataaatgtc tagctgctct tattgcaata
     6361 ccagtagcac ttcaaactgg tgttaatgca gaagttgcag tatctattgc ggtaccttta
     6421 ggtgttatag gagtattagt taataactta agaagaactg gtaatgctgt attggttcat
     6481 aaggcagata agtatgctga agaaggtaat acaaaaggca tctggagatg tgcaacactt
     6541 tattctttaa tatttgggtt tgttttaaga tttccaatag tttttgtatg taactttttt
     6601 ggagcagatt tagttcaatc attattagat gttataccac aatggttaat gaatggatta
     6661 acagtaatgg gcggtatatt acctgcatta ggttttgcaa caacaatatt tactataggt
     6721 aaaaataaat tcttaccaat gtttattata ggatttttta tggttcaata ttttgaaata
     6781 tcaattactg cagctgcaat atttggtgtt tgtatagctc ttttaattac ctttatgaaa
     6841 gaagacaaaa gggtaggtga agtataatgc aagaaataaa aaataaagaa gttaaaaatg
     6901 gacaaggtcc aactgaagtt aataagacaa gagtactcac aaagaaagat gtaacaaaaa
     6961 catacctaag atggtggtgg acagcagagt tatctaattc ttttgaaaga atgcaagcat
     7021 tagcagtttg tgcaagtttc actccggctt tagaaaagtt atataagaaa aaagaagatt
     7081 tagtagatgc attaaagaga cacttacaat tttttaatac tcaagctata tggggaggat
     7141 taattcatgg tacagtttta gctatggaag aagagaaagc tactgaagga aaaatcccag
     7201 gagaagttat ttcaggtgtt aagaatggtc ttatgggacc tttggctgga attggagata
     7261 ctttagattt tggtacattt caaacaatat tcttagcttt aggtgcttca tttggagcag
     7321 aaggaagtgt tataggtgca tttttcccaa taatgtttag tatattacta ttttgcgaag
     7381 gttattactt atttcattta ggatattctt taggtagaga ttcaattaag aaaattctat
     7441 caggtggtat tgtcaataag ataattgatg gtgcttcaat tctaggaatg tttatgatgg
     7501 gagcattatc tgcaactaca gttaagcttt caactccgct atcatttgat attggtggta
     7561 aagcaattgt tgttcaagat acattaaata tgattgctcc aggtttatta ccacttggtg
     7621 ttgtattctt cgtatattgg ggaatgaaat acaagaagtg gacaataact aagttattag
     7681 taattttagt tgtattagca ttggtaggat cattcattgg aatattctaa ttcacagtga
     7741 ttaaaatata gaatttaatt ttaggaggag aaagaaatgt cagaaaaaat gtatacagaa
     7801 aaggaatatt tagaaggaat tagaggagca acaggagaaa gagcagtatg gttctatctt
     7861 cttatgaaag aagctgaaaa attaggagct aatccagatg atatatgtaa agaagcaata
     7921 tatggatttg gtaaaatgag aggtcaaaaa tataacgtag cagatactcc aggaaaaatg
     7981 gcagaaatgt tatataacag taaaggacaa aaagtgtttg aaatggaatt agtagaaaat
     8041 acagatgaga atggcgtttt aaaattccat cactgtccat tagatgctgc ttggaaagaa
     8101 tatggattaa ctaaagaaga aagaaaagaa atttgtagat tagcttgtta tggggattat
     8161 ggaagagttg actgtgcaca aggtgttaaa ttagattttg ctcaaaaatg tgctcatgat
     8221 gatgaagttt gtgaattagt atttacaaaa aaataatttt taatgctaaa agcctgttct
     8281 ttaaggacag gctttaaaaa gatagaaagt aggttgattt atggatataa gcagaagtgt
     8341 agataatagt ttaaatagaa ccatagaatt tctaaaagaa ttgataaaaa ttgattcaca
     8401 acaaggtgaa cctatttcac aatgtccttt tggtatagga cctaaaaaat ctttagataa
     8461 aactttagat tactgtgcta gtttaggttt tagtgttaaa aatattgata attatattgg
     8521 atatgcagaa attggagagg gagaggaatt aatagggatt ccaatgcatt tagatatagt
     8581 tccaccagga gaggggtgga gtgtagaccc attttcggga gcagtaattg acaatattat
     8641 ttatggaaga ggtgtcatag ataataaggg tgctgtatct atgcttatac atgtattgaa
     8701 aaacatagag gatatgtatc ctacaattaa taaaagaata agattaattt ttggtacaaa
     8761 tgaggagaca gatatgaaat gtatcaaata ttacttagat aaaggtgaag aaattccaag
     8821 tatgggattt actccagatg ctatgtatcc agtggttaat ggagaaaaag gaagggttca
     8881 tataaggata gaaaaagaaa ttaaaattga caaaagtaaa ccttacataa tagtaagggg
     8941 aggaactaaa gaaaatgttg ttccatctca ttgtactgca aaaattatta atggaattat
     9001 ttcagagtta actactaaag gagtagctgt acatgcaagt aatcctgaaa agggagaaaa
     9061 tgctatatct aaaatggtaa taaagatagt tgaggataat atggattttc agcatagaga
     9121 agatatagaa ttagtttcaa aatacctttg tagtgattat tatggagatg cattggggat
     9181 taatcaatat gatgaggtat tcaaaaacac taccttaaat ttaggaatat taaaagtaaa
     9241 tgaggaaaaa atcgtatgtg agttagatat aaggtatggt aaaaatatag ttttaaataa
     9301 cataatagat agattcaaaa aggttttttg taatggttgg aaaatagagg ttattgctca
     9361 taaagactta cattatgtgg atgagagtaa tttagtactt aaaaaattat tagaagccta
     9421 tgaagaagta acagatgaaa atggatatac aattgctatg ggtggtggca catatgcaag
     9481 ttggtttaaa gatatggtgg cgtttggacc taagttctta gcgtataaaa ctggtggtca
     9541 tggagtagat gaaagagtac caataaatca tatacgaaag aatatggaga tatatacttt
     9601 ggctttaata aaactattag aattgtaaaa gaagaagaga ctttatgtgc aaatttacag
     9661 atatcttaaa aagagagata aaacgagttc aactttattt aaatgttcaa caccatcaaa
     9721 tggggctgtc tcaaaataga gataagcctc ttgcttatta ttgaaatgat gaaaatatat
     9781 tttacttcat acaaaaaaga gtgccttatg aagtaaaaag ttaattttga gacaccctca
     9841 tttgatgatg ttgaagagaa aataagtatg aaattattaa aaaaagatga ttttataaaa
     9901 gttaaggttg aaaataaaag ttaattgaat ggaattagat aaatggagaa ggctgtatac
     9961 tatctaataa aataacaaaa gagtgttata aaactggtta tatgattaga gaaaagtcta
    10021 ttgatactta tccagatagt ggacggggat ttatgacttg ggatgaaaat ggagaataat
    10081 aaaaattata gtttatacaa tttaataaga tatatgtaca agcagttaat ctaaatgatt
    10141 gactgctttt cttttataga tttttatata atagacaatt actaatacat gatagttgcc
    10201 tgttataatt agaggaatag atacgaataa taactttata aaagaattaa atggaaaatt
    10261 gaagagttat attcaggatt ttaaaaagaa tatgaaatga gaaatgaaaa taaggtgttt
    10321 atattaaaag aggttttaca caaaatactt aaaaacgtga taaataaaaa ctgtatttag
    10381 ataaattaac tactagtttt atatataata tggattttgt ttacaaagtt tacatatttc
    10441 acaaaatatt tttaaaatat atttaaaaat atattttata tacttgttcg tttgtttctc
    10501 tgtggttgat tttttcgtca aaagtcaact tcaaatcgct tgttagcttt acatgtttaa
    10561 ggatataatt atacataagg agggattgac atgaatgaat taaacatcgc aaaaacgctg
    10621 attttaaagc gtaaagaaaa aggaattaca caagatgaac ttgcaaatta tataggcgtt
    10681 tctaaagctt ctgtatctaa atgggaaact ggacaaagct atcctgacat aaccttttta
    10741 ccgcaactcg cgacatattt caacattacc gttgatgagt taatatgtta tgaacctcaa
    10801 atgatgaagg aagatattaa taaactgtat aataaactgt gcaaagactt tacggttaaa
    10861 ccgtttgatg aagtcatgga tgaaataaga gaaatcataa aaaaatatta ttcatgtttt
    10921 cctttgattt ttcggatggg tcttttaata gttaatcact atgatattgt agatgaaaaa
    10981 aagcgtgaac tattaattag tgaagcatta gaaatattta ttcgtataca ggaaacttgt
    11041 aatgacatag atatttgccg tcaagcaaaa agtatggagg caacttgtta tatattgtta
    11101 aatcaaccca tacaagtgat tgatttattg caaaacagca attttcccat gataaatgaa
    11161 tctatacttc ttgcacaggg gcaaatgatg aatggtcaaa tggatgaagc aagagaaaca
    11221 tttcaacttg gtgcatatca gaaccttata tcattagtcc aaaatctagt gggtattttg
    11281 caaaatgcag ataaattaca aatgaaggaa atagaaagac gtattctagc aatttcagat
    11341 atatttgagc ttgatacatt atctcctgct attatgcttt cttcatattt aacacagaca
    11401 caaattaatt taatacatgg ggataatgaa ggagctatta aatcattacg aaaatatgta
    11461 gatttagcaa cacgtgatat atacccaatt ataatacatg gggatgactt ttttaataaa
    11521 cttgacagat ggatttctga aattggaaca ggtattacac gtgatgatac aatcgtaaaa
    11581 gcaggtattg ttgctgctat taaaaataat ccaatgtttt ctttattaag tgaaaacaaa
    11641 gaatacaaat ttcttataga aaaattatct ttattggagg aataagatta tggaaaattt
    11701 gatggaatat ttaccacttt tgattcctgt tattatttta gatttaatac ttataattac
    11761 tgcacttgtt catgtattga gacatccaaa ttataaaatt ggaaataagg caatatggat
    11821 tattgtagta ctgtttatta gtctaatagg accaatcttg tattttacaa taggaagagg
    11881 tgaagaatga tgaatattgt ctcaataaat ggattatcaa aaggttttgg taatcgaaag
    11941 ataatagata atctaaattt tactgtacca gaagggtcag tctttgggtt tgtaggtaag
    12001 aatggtgcag gtaagactac aacaatgaaa atggtacttg ggttacttga accagatagt
    12061 ggaactatag atgtttgtgg tgagaaagtg acatatggaa gaacaagttc taatcgtcat
    12121 gtaggttatc tgcctgacgt tccagagttt tacaactata tgagaccact tgaatatctt
    12181 tctctttgtg gtgaaattac ggggctttca aaaaaagaaa taaagataag gagtgaagag
    12241 ttgttatcac ttgtggggct tggcaatgaa aagagacgaa taggtgggtt ttctcgtgga
    12301 atgaagcaac gacttggtat agctcaagct ttgctttcac gtcccaaatt acttatttgt
    12361 gatgaaccaa caagtgcact tgaccctgtt ggtcgaaaag aaatattaga tattatgctt
    12421 aaaatcaaag actcaacaac tgtaatattt tcaacacata tactatcaga tgttgaaaga
    12481 atctgtgacc atgttgctat actaaacaaa ggaagcattg ctcttagcgg tacactttca
    12541 gaaataaaaa gtatgcacgg aaaggataga cttttactgg agtttgcaag taataatgaa
    12601 attcaaaagt ttaagtctag tggtggcatt aaatccttac taaaagattc agaagaaact
    12661 aatatggaaa ttgttttgca tggtaaagat ataaaagcaa tacaaaaaac agttatatct
    12721 acactcgcag aaatgaatct ttgtccagta aagatggaac taatagaatt gtcacttgaa
    12781 aatttgtttt tggaggtagt aaagtgagag aatacatagc atttacaaaa aaagagttta
    12841 aggagaacat aagaaattat aaattactta gtttgactat tttattttta gtatttggaa
    12901 taatgagtcc tctttctgca aaatttatgc cagacttgat tgctcatttt gctccaactt
    12961 taaaagttac tactgcacca actgctttag attcttggac acagttttct ggaaatattt
    13021 ctggtcttgg aacgagttta actctaattg ttttttgcaa tattttatca aacgaatatt
    13081 ctaaaggaac actcgttatt atgttgacta aagggttatc acgttcatct gtagtattgt
    13141 ctaaattttc tattgcagta attattatga caattggttt ttggttgagc tttttatgta
    13201 cttatggata tacaatgtat ttttggccaa ctgcaaatct taatcatatt atattttcag
    13261 catttaactt atggttaatt agtattatgt atatttccat attaattctt gggtgtgtac
    13321 tatttagacc agcatttgca agtgtgttac ttgtattagc agcaactgct attttgagtt
    13381 tgatttctat tcctaaacag attgcacctt atacgcctaa tttcatatta tcaaaaaata
    13441 tagatttagt ttcaggtaaa gtgacacctc cagaatttat tattccaatc attgtaacaa
    13501 ttgttatttc gatagtatgt ctgttattag ctgttgtttt gtttaataaa aaagcagttt
    13561 aaggatattt ttattataaa tacaaatagt gctgtattgt gggaaaatgt gtttagtatt
    13621 gtagaagaat atatttaagt aaaaaagagg ctgtctcaag atagagaaat aagcctcttt
    13681 tttattttag tcctcctcac tatactttat aataattaag ttattacctt aaggaactat
    13741 ctaaaaaaag agatatagat tataatctaa tgcttatcag ttatttgagg tgtttagtat
    13801 atagagtcta aagtagtgaa ggactaaaat aatattgttt tcattaaata aagataagaa
    13861 tctttaattg tggtaaaata tactaaaaaa tcattttaaa agtgataaaa ttattttaag
    13921 taaatatttt aaaggggtgt agatatggaa aagtcaaaag tatatttttg tgatttatac
    13981 tcaaattcgc aaaataaaaa cgtaccaaac aatgtaagaa gattatttga tgaagcagga
    14041 ttcaaagatt taatagaaaa gaatgaccaa gtcgcaatta aacttcactt tggagaaaag
    14101 ggaaatacta catatatgag tcctgtggca gttagacaag tggttgataa ggttaaagac
    14161 tgcgaaggaa agccattttt aacagataca aatacactgt atacagggag tagaacaaat
    14221 tctgtagacc atcttacaac tgctatagaa aatggatttg cttatgcagt tgtaaatgca
    14281 ccagtaataa ttgcagatgg attatatagt agaaattatg agaatgtaaa aatagacaaa
    14341 aaacattttg aaagtgtaaa aataggtgga gaaatttata attcttctgc aatgatagta
    14401 atgagtcatt ttaaaggtca tgaagcagca ggttttggag gagcattaaa aaacttagct
    14461 atgggatgtg caagtgcagc aggaaaacaa atgcaacatt ctgatgttac tccagtagta
    14521 aaggaaaaga agtgtgtagg ttgtggaaag tgtgttaatt cttgtcctac aaaagctata
    14581 agtatagtgg ataaaaaagc agttatagat tctgatgtat gttatggatg tggagaatgt
    14641 ccaactgtct gtccaactag agcagtaact atacaatggg aaagtgactc agatgtgttt
    14701 gtcgaaaaga tggcagaata cgcttatgga gcagtatcaa ataaaaaaga caaggtaggg
    14761 tatataactt ttgttatgaa tgtaactcca ctttgtgatt gtgtaccttg gtcaggtaga
    14821 ccaattgccc atgatatagg aatattagca tcaacagacc ctgtagctat tgaacaagca
    14881 tgctatgatt tgatttgtaa ggaaatgggt catgatgttt tcaaacatga acatcctcat
    14941 gtaaatggaa ctagaataat agactatgca tgtgaaatgg gtatgggaag taaagaatat
    15001 gaattaataa aattataaga atagtagcac ttaaatgcat ataattattg taagagaaat
    15061 ctaaaagtat caaaggagta tgcatttatg aaaatttact atgagaagga aaacagcaag
    15121 aaggctaatg taaacaatat tccaagtgta tttgcgttta attttctaag aaactttttg
    15181 actagcaaag agtatgagga aactagaaaa gaatatttta taaataatct aacaaaaagt
    15241 caggttaatc aagccatgaa ggatttaaaa tggctattta gagaatatga aggcttagag
    15301 gttattacaa tggaaagctt ggatggagtt aagacaagga tagttttata aaccagtaag
    15361 aattatattc taataaaaaa tactgcttta ataataatat gtataatcta tatactaata
    15421 ttattttgaa gcagtatttt tttgtgtata gtaaacttgt gtctaaaata aagttgacca
    15481 ataaatgtta tatatgcaat actatatata ataagtaaac tacataaaaa tattaagttg
    15541 acaaatagga ggatatatgg aaaaatacat aataaaatta aaaaataaag tattgagaga
    15601 aaaagaaata agttatgaag aagctcttaa tttaataagc cttgatacaa ataataaaaa
    15661 cgattttgat attctattaa aatctgcaaa tgaaataaga gaatatttta tgggaagaaa
    15721 agcagattta tgtacgataa tgaatgctaa atctggcaaa tgtagtgaag actgtaaatt
    15781 ttgtgctcaa tcatctcatt ataaaactgg tgtagaagaa tattcattat tagattacaa
    15841 tgaaatctta aatagagcta aagagatgga gagtaaagga gtgcatagat tctcattggt
    15901 tacaagtggt aaaggtatgt caggaaagga gtttaataat attttaaata tctatgaagg
    15961 attgagggaa aatactaatt taaaattatg tgcatcttta ggtattatag attatgagca
    16021 agctaaaatg ttgaagtcag caggtgtgac tacatatcac cacaatgttg agacgtgtag
    16081 agataatttt cataatatat gtacaacaca cacatataaa gataggataa aaactataaa
    16141 agatgctaaa aaagctgggc tagatgtatg tgtaggcggt ataattggaa tgaatgagag
    16201 tgaagaacag agactaaaaa tggtttttga aatacgagaa ttaaatgtaa aatcatttcc
    16261 aataaacatt ttaaatccta ttaagaatac gccaatggaa aattatgatg tattagaacc
    16321 aatggaaata ctaaaaacaa cagcagtatt taggtttata attcctaatg tgtacataag
    16381 gtatgctggt ggaaggctat cattaaaggg atatgacaaa gttggattta atgggggagt
    16441 aaattctgca attgttggtg attatttgac aactgttgga agtgggattg aaaatgataa
    16501 aaaaatgatt atagagcaag gatttgaact ttaataaata ctacttaaat taattaatat
    16561 acttttagat aattataaaa gtatattaat tttttttgca gattaattta aatattaaaa
    16621 ttgaattata agttgcaata ttttgaaaaa agggaaaaat atcaaaattt atataaaatg
    16681 gttgcaaatg ggaaaaggat gtgatagtat ctaggtaacc ggttaagtaa aacggtttcc
    16741 taaattttgg gggtgaactt cttgagtata actataaaag aaattggcga attggctggt
    16801 gtttctaaaa caactgtgtc aaaagtcatt aataataaag atgaaaatat aagtcaggca
    16861 acaagagaaa aaattttgaa aataatgaag gaaaaaaatt atgtaccaaa taaattagca
    16921 caaagtttag taactaaaaa aactaatact atagggcttc ttataccaga tatacgaaat
    16981 ccattcttta cagatgtatc aagaggtgta gaagacaaag ctaatgaaga aggctacaat
    17041 ataattttat gtaatacaga tgaagatgct aaaaaagaat atgaaggaat aagaacacta
    17101 tcagagagga tgatagatgg aattatattt gcagcatctt caaatacaaa ctggaaagaa
    17161 gcaaattata aagatataaa aataccaact gtcttgattg ataagaagat aagtatgaac
    17221 aaagaaacct taaaaggaat tgttaaaata aataactttg aaggggccta tatagctaca
    17281 aaacatttat tagatattgg aaataagaaa ataatatacc taagtggtcc acttcaaaat
    17341 gaaatagcag tagatagatt agaaggatat aaaaaggctc taatcgagtg taatttaagc
    17401 tacaattctg cttatgtatt tgaaggaaaa tataagattg aatggggaca agaatttata
    17461 aaaaatttag aaaagataga ttttgatgca atattttgtg caaatgattt gattgcaata
    17521 ggtgtcataa gaggtcttaa agaaagaggg ttaagtatac caaatgatgt aagtgtcgtg
    17581 ggatttgatg atattcaaac atcaagtcta ataagccctt ctcttaccac agtaaaacag
    17641 ccgtcttatg atataggata taaagccagt gaaatattga taaattgttt aagaggagat
    17701 aaaaaagaat catttgatga attgattttc aaaccagaat tagtcataag agattcaaca
    17761 aaagaaagtg aataacagca cgagagacat aaatagatat gtaaaggagt aatgtaatgg
    17821 gaaacatagt tgtcatagga agtgtaaaca tggacatggt atgttctgta gataaaagac
    17881 cagaaaaagg agaaacagta ttaggtaata gtttttttac atcacctgga gggaaagggg
    17941 ctaatcaagc tatctcagct tctaaactag gagcaaatgt aaaaatgata tcatgcatag
    18001 gggaagatgg cttaggagag gagttaataa gaaattttag aacgaataaa gttgattata
    18061 gtttagtatc cagaaataat cacaaaagtt ctggtgttgc tgttataaca ttatgtgaaa
    18121 atgataatag tattgttgtt gtaccaggga cgaatgagct agtagatata gaattaatta
    18181 aaaagaatga agaagagata aagaatgcag atatagtatt gctacaatta gagattccat
    18241 taaaaacaat aaattatgta gtgaatttct gttttgaaaa taggattaag gttttattaa
    18301 atccagcacc agcagtaaaa ctaaatgaag atataataga aaaagtaact tacttaacac
    18361 caaatgaaca tgaatataag atagtttttg acacaaatga agggatagaa gaagtattaa
    18421 aaaaatatcc aaataaactt gtaataacag aaggaaaaaa tggagctaga ttttatgatg
    18481 gtgaggaaat caagcatgta tcttgtataa gtgttgatgt tcaagatacc acaggagcag
    18541 gagatacatt taatggagca ttggcagtgg ctataacaga agggaaaaaa ttatatacag
    18601 cagtagaata tgcagtagta gtatcaggtc tttctgtaac taaactaggt gcacaatctg
    18661 gtatgcctta taaagaagat gttgaaaaat atttaaataa taaataaaaa tctaatttgt
    18721 tcaaatttaa aactattaaa aatggaggtt aattatgaga gtgagaaaaa ttttatcttt
    18781 aggaattgct ggaatactag caataggaat gttaacaggg tgttcaatgg aaggaccaag
    18841 taaatcagat aataaaggtg gttcagacaa gaaagattta actattggag tatctacaat
    18901 aactcttcaa catcaatttt ttatagatat tgatgaggga ataaaagaaa aagctaagga
    18961 attaggtgtg aaagttatag taaatgaccc agaccaagat gtagctaagc aaacatcagc
    19021 aatagaagat tttatacaac aaaatgtaga tggaatgata gtacttggaa ctgataattc
    19081 ggctatagta ccagcagtag aaggagcatt tgaaaaaatg ccagtagtta cagtagatgc
    19141 tgtattaaat actgaaaata ttacaagtta tgtaggaaca gttagttatg atgctggtaa
    19201 aaaattaggg gaatacacta aaaaatacat agatgaaaag ttaggtggaa agtcagaaat
    19261 agcaatagtg acagatttaa aatctcaaat acaaatgcag agaatagatg gatttaaaga
    19321 tgcgttaaaa ggttcagcaa atgttaagat attaaattca caaccaggat atgacagaga
    19381 agaatctctt aatacagtag aaaacttaat tcaatctaat ccagatgtag atataatata
    19441 tgcaacagct gaaaatagtg tactaggagc taaagctgca cttgaatctg caaaaaataa
    19501 ggatgttaag attgtaggat ttgacttaac tgaagaagca tcaagtggaa taacagatgg
    19561 aactattctt gctatgattc aacaacaacc taaagagatg ggacgtttag ctgtagaagc
    19621 agtagtaaaa gctataaaag gggaaaaggt agaaaagaat ataccagtac cagctttact
    19681 ttatgataag gaaaatataa aagactttaa gaattagtaa aataaaatga gattatttga
    19741 aagaaatcct atttaaaaag gagtgtcatt atgagtaata taattctgaa gttaagtaat
    19801 attgctaaag aatttcctgg tgtcagagca ttagataatg ttaattttga gttatttcat
    19861 ggcgaagttc atgcactact gggtgaaaat ggtgctggta aatcaacaat gataaagatt
    19921 ttaactggtg cacattcgaa aacttctgga aaatttattt ttgaaggcaa agaaatagag
    19981 aatatatccc cagatatctc taaaaagata ggtataaatg ccatatatca agaactaaca
    20041 gtttttgacg aattaacagt agcacaaaat atttttatgg gaaaagaaat aaatggcaaa
    20101 gtcttaacca atgacaaaaa aatgaatgaa gaggctaaaa agattttcga taatatggga
    20161 atagatataa atccaaatag tttagtcaaa gagcttagta tagctcaaaa acaaatggta
    20221 gaaatagcaa gagttttatc atcagaaact aaagtattaa taatggatga acctacttct
    20281 tcaataagta aaaaagagac tgaaatactt tttagactta taaatgattt aaaagagagt
    20341 ggagttagta taatatatat atcccatagg atggaagaac tctttgagat ttgtgacaga
    20401 ataactataa tgcgagatgg gaaaacgatt tcgactttga ataccaaaga tgtatcgtct
    20461 gaggaagaat tagttaattt aatgatagat agaaagctag accaattttt cccaaaaaga
    20521 aaagtagaaa taaaagaaga aattatgcga gtggaaaatc taactaagaa caatgttttt
    20581 aatgatatta gctttaatat aagaaaaggt gaaatattgg gaataggtgg actagttgga
    20641 agtaagagaa gtgaaatagt agaagctata tttggactta gaacatatga ttctgggaaa
    20701 atatatttaa acaatgaaga agtaaagttt aaaactccat cagatgcaat agaaaatgga
    20761 ctgggattaa taacagaaga tagaaaaggt actggattgt ttttacaaat gagtgtcaaa
    20821 gaaaacacaa ctatggctgg gttaaaaaag atatctaagt ttaaatcaat tatagataga
    20881 aaaaaggaaa aagagatttt agaaaaatac atagaagctt taaagataaa aacaccacat
    20941 atgaatcaag ttatacaaag tttaagtggt ggaaaccaac aaaaagctat aatagcaaga
    21001 tggcttttat tacaaccaga tatattgatt atggatgaac caaccagagg tatagatgta
    21061 aatgctaagg cagagatata caatctaatg ggagatttag tggagagtgg tgtaagtata
    21121 ataatgatat cttctgaaat accagaatta atatcaatga gtgacagaat aatggttatg
    21181 agagaaggtc atataagtgg atttttagaa ggtgaagaaa tggttgaaaa taatgtatta
    21241 aaattggctt tcggaggtaa aattaatgag ttcaataact aagcataaag aagaaaaatc
    21301 ttctccatta ttgacaaaat taacaagtgt atttagagac caagggagtg cagctgtagg
    21361 acttgtaata atttttataa taatgagtgt agcttcatct aactttttaa ctttagataa
    21421 tttaattaat gttggaagac aaatatcaat aaatgcaata cttgcagtag gtatgacatt
    21481 tgtaataatt acaggaggaa tagatttaag tgtaggagca gtaattgctt tagtaggtac
    21541 attttgggct acaacagtag tgaattataa tgcacctata tgggtaggta tgatacttgc
    21601 attagcaata gggacaatat taggagtcat aaaaggtgca attatttcaa cacaaaaact
    21661 acctccattc attgtaactt tagccatgtt aacgataata agtggggcaa gctttgtatt
    21721 tacaggagga agacctatat cagtaaatac agatgcattt aagatgctcg gtagaggata
    21781 tattggacca ataccaatac cagttataat tatgattata gttgtaatag caggacattt
    21841 tctattaaaa agaactaatt ttggacgtca tgtacatgca gtaggtggaa atgaagaggc
    21901 cgctagatta tgtggagtaa aagtaaataa agttatagta aaagtatacg cactagctgg
    21961 actactaaca gcattagctg ggataatact atcatcacgt ttggcatcag gttcaccaac
    22021 tgttggagat ggagcagaat tggatgctat agcagccgtt gtgcttggtg gtactaatat
    22081 gatgggtggt tctggttcta tagtaggtac atgtataggt gtcggaataa taggaatatt
    22141 aggaaatgga ttaaacttat taagtgtatc ttcatataat caaatgatta taaaaggttt
    22201 ggtaatgtta tttgctatat ggataaataa tataaaatta aaaaaatcag ctaaatctaa
    22261 atagtaatta aattttagct tataaactat aaaaattatt aagtttttgt atatattttt
    22321 atactttaat ttgcatacat ctttatacat tgaatgggaa tatgctaagt gaaaaagttt
    22381 actatggctt aaatagatga gattagactt gaaattttaa atgtaaaaat aaatcttagg
    22441 aggaaatatg aaaaaaattc tcttagatac attaaatagt aaaaaacaag aatacataga
    22501 ttatttaaaa gaacttgtaa gtataaagac tgaagatgtt ggtcatggaa tactaggtgg
    22561 atttgaaaaa gaaggacagg aatatattga aaaacttgca aattacatag gttttagtgt
    22621 agatagacaa gaaatgagcg aagaattaat taaaaaagca aaaaatatat ataaagaagg
    22681 aaatctaggt cacaattatc aagatagata taatttaatt tgtaagtata gtgatgattt
    22741 acctggtaag acaattgtat ttaatggtca tgtagacact atgccaccag gtgatataag
    22801 taagtggaaa tataatccat atcgtgctac agaagataat ggaaaacttt atggtcttgg
    22861 aactgctgat atgaaatctg gacttatagc atcaatatta gctgtaaagc ttataaaaga
    22921 tagtggtcta aatgtacctg gtaatgtaaa aataatgtca gtagtagatg aagaaggtgg
    22981 cggaaatggt accataaatg cagttatgaa tggtattggt ggagattgtt gcataatttg
    23041 tgaaccatca gaacaaaact taatagttgc acatatggga tttgtattct ttgaagttga
    23101 ggttaaaggt gtgtctcttc actgtggtag taagtgggag ggtgtaaatg caattgagaa
    23161 ggctatgttg ttgcttcaag atataaaaga gttggaacat aactggctta tgatatataa
    23221 gcatccacta ttaccttcac ctacaataaa tttaggtgta ataaatggtg gaacagcagg
    23281 ttctactgtg ccagataagt gtgtatttaa tttatgtgta cacttcttgc caaatataat
    23341 gagttatgaa caagttgtaa atgatgtaac aaatgtcata atgacaaggg ctaatggaga
    23401 tttatggctt aaagataata aaccaaatat aaacatatat caatcagggt taggttttca
    23461 gatggacaag gattctgatt ttgtagtgaa tgcacataaa atactagaag aaactttagg
    23521 aaaaaaatta gagataaaag gttcaacagc tggcaatgat gcgagattaa tgaaaaattt
    23581 agctgaaata ccaacactta tattagggcc aggttcaata gaacaatgtc attcaattga
    23641 tgaatatgtt gaaattaagg agtatttaga tagtatactt atgtatgcaa gtctaatatt
    23701 aaacttataa aagtaggagg aatatataaa tgaaaaaagt attaatagta ggggaatctt
    23761 gggtaaaaaa tataactcat ataaaaggat ttgatacgtt tgttacaact cattatgaag
    23821 aagctgtaaa gtggttgaaa gaagcaatag aatctggagg atatgagaca gttcatatgc
    23881 ctgcacatgt agcagcagat agttttccgt ataaattaga agagttaaat gagtatgact
    23941 gtataatatt atctgatata ggttctaata cgttcttatt atctaatagt acttttatag
    24001 attgcaatag taatccagat agacttgaac taatcaaaga gtatgtaaat aatggtgggg
    24061 cgttaatcat ggtaggtgga tatatgtcat ttacaggaat agatgcaaaa gctagatttg
    24121 gagaaactgc tatcaaagat gtattaccaa taacaatgat agataaagat gatagagttg
    24181 aaaaaccagc aggaataatt ccagaagtta ttgactctga acatccagta ttaaaaggta
    24241 taccaacaga atggccaaag tttttaggat ataacaaaac agtagctaga gataattgtc
    24301 ctgttttagc aactattgga ggagacccat ttgtagctgt tggcgagttt ggaaaaggaa
    24361 aatctgctat ctttagttct gactgtgcac ctcactgggg tccaaaagaa tttactgatt
    24421 ggaaatacta taataaatta tgggttaata tgcttgattg gttaacatgc taaaaatttt
    24481 ataaaacttg aaaacctctt acttgataaa atgaaactta caagtcaaga ggttttattg
    24541 tataaaatgg ttgtatggta gaaaatattt ttaaatcagt taaaattagt cattttggct
    24601 gataatcgat aagttattag aaacagggag gtttatataa ttaatatgga taatttaaat
    24661 aaaaagtttt tgatggaata tatagatgag atacagggag aattaagaga tatatcagat
    24721 aagatatgga aaaatccaga acttcagtat aaagaatact atgcttcaaa tttacaaaaa
    24781 gaatgtttaa aaaaacatgg ttttagtata gagggattag aaggtatgga tacagcattt
    24841 gtggctagtt atggagaagg tagaccagtt atagcagttt tgggagaata tgatgcttta
    24901 gatggtttgt ctcaaaaagt atccactact aaagaaccta ttgtagatgg tggtgcaggt
    24961 catggatgtg gacataactt acttggtaca ggttcaatag gagctgtaat tgcagtaaag
    25021 gaattaatca aactaaaaaa gctgaaaggg actataaaat actatggatg tccagcagaa
    25081 gaagatttat ctggaaaagt attgatgata aaaaaaggtt tttttgatgg aattgattgt
    25141 gcttttagtt ggcatccatt tgatataaat actccgataa gaatcccaac acttgctaat
    25201 tactctgtga aatttagata taatgggatt agtgctcatg cagcacaagc tccttataac
    25261 ggaagaagtg cacttgatgc agtagaactt atgaatatag gttgtaatta tttgagagaa
    25321 catatatttg ataattgtag agtacactat gtaactacta atggtggaaa aatgccaaat
    25381 atagttcctg attttgcaga agtgtggtat tacataagag gagttaaaat ggagcatgta
    25441 agagatgtat ttggtaggat tgttgatata gcaaaaggag cagcattaat gactggtact
    25501 attatggaat atgacattat aagtggagta tatgactata ttccaaatac aatattaaca
    25561 gatattctat cacaaaatat gaaattagta ggtgtgcaaa actataatga agatgactac
    25621 tattttgcag ataaattagc tgagacagtg tctatagata aaagaagtag tgtttctagt
    25681 gtattaagtg gaaataagga tattactaag atgaatttac atgatgaggt tacagatgat
    25741 acatttactc ataataactg tatatcatta tcattggata ttggagatgt tagctacata
    25801 attcctacag cacaatgttc ttgttcagtt tggcctattg gtatttctgc ccatacatgg
    25861 caatcttgtg ccagtgcagg ttctgatatg ggatttaagg caatgttatt ggcatctaaa
    25921 tctatatcat gttcaataaa tgatgtaatg cttgatgaat tggttataaa taaagcaaaa
    25981 aaagaattga aggatactgt aggtagtttt gaatatactc caattatttg aagagtaatt
    26041 taatacaagt aagattgaat tgtatgaatt aggatgaata tataaattaa tattattttt
    26101 aacttaactt tctaattatt ttcaatctaa gatacaataa ttagaatata tttttaaagt
    26161 ttcctattta aggaaaataa tttgatttta tgtttgataa aaggacttat aatttgtaag
    26221 cccttttatt atatttattt tttgtttata attaatttta ttttttaggt ttagttaggt
    26281 tatagattac cctgaaaaat aacatttaac ttattattaa aaacatgtaa gaatagttta
    26341 taaatatttt attttcgatg ttgtttatta tatccatttt atcgatggat gaggaatggc
    26401 tctgccttcc ctagttacag atgtagtagg gaaaccaatt gcacaggcat caaatccata
    26461 ttcacctgtt ggtactccta aaatatcaca aaaatcttta ttatcgctca atcttcctat
    26521 tacactaaca agttcaaaac ctaatcctaa agcagttgat ttaagccaca tattctgcat
    26581 cacatgagat aatgactttt gttctatagg tggtattcca cgacgttctg ccacaataat
    26641 aagataaggt gctgttccta taccaagaac ccctttttca acttgcattt ttaaggaagt
    26701 aatgaaggat tgaagggtaa tgttgttctt tgccttcttc tcattgactg atatttgagt
    26761 tttaataaca tcaacaagaa tggtttttat tttattcatt tgtaaactgt tatttgaaat
    26821 aacaaagaaa tgtctgaaat ctgtttgttt tgctactgaa agtattgcat aaggtgcaag
    26881 tcttccagtt tcaattattt cttcaatcag cccttttgaa ggtacttctt ttgtaaaact
    26941 tcttatggat cttctagttc ttaatagcat atccaaaact tcattaacat tgtttttatc
    27001 taaattgttc atttttatcc tccactaata gtattcttaa gatgttctat aatgcatcca
    27061 tataaaggcg aacttttcgg tcatattaaa catttccttc aaattaagtc gctatttcta
    27121 tgaacacaat gttgattaat aatctatatg tatattataa tatttatatt gcattatgca
    27181 ataaacaata atatttaaac tgttaagtaa taaacattta ctataaaatt gttaattaaa
    27241 attaaaaaaa tttatatgga atgaggaaat aattcatgaa aaaagtatct attgatagaa
    27301 gaactatacg tactaaaaaa atgattaggt ctgcattagc agagttaata gatgagaaag
    27361 gatttaacaa tatatcagtt actgatttaa ctcaaagggc agatataaat agaggaacct
    27421 tctatctgca ttatgttgat aagtatgact tgttagagag gattgaaaat gaaataatac
    27481 aggaaattga agaggagacc aaacatcttg attcatccaa catgatggag atagatacct
    27541 caagtgaacc tcttccattt atggtaaagc tttttgaata ttttagaaaa aattcagtaa
    27601 ttattaaatc tatcttgggt cccaaaggtg accctacttt tgaaagaaaa ataaaaaaat
    27661 ttattgaaac caatttgttt gagaaacaaa aactaataaa ttttaacata gatgaggcaa
    27721 taatttcaga agaatatttt atacaatata tactttcagc agacttaggt gtaattcaat
    27781 attggcttga aaaaaatatg aaagagtccc ctgaagaaat ggcactgata cttgctagaa
    27841 tgtcgttact cggtccgtta agagcagtgg gaattcgtaa agattcataa aaatagtttt
    27901 atatatatta gactttgaaa atatatgttt aaaaaccatt ttttactact actgtttata
    27961 aattagggga gatatcttaa aatattagta aaaggagtgc taagtgagtg taataactat
    28021 gagataagag gtttagttga tatagtcaaa acttcaaaag aatcaattag aaaagttaaa
    28081 atattgcaat taaatttaaa ggagtaatat atgaaaaatg aaaatataag ttatttggcg
    28141 ttaggattat gttttggtgt tgttttgggt tcattattta aaaacttagc tattggttta
    28201 tcctttggat tagttattgg agtagtatta gacagtaaga aaaataaata aaacttcaat
    28261 agtaacatat aacaattata ctatttttgt ataaaatcat tggaaatcaa caatagaaaa
    28321 tatttgttat ttaaaaacta aaaatacagt ttaaacgacc taaaaatata ttatataatt
    28381 attataacaa gataagtgag agtaaggtta tgataataga gtaatatttt tcaattatta
    28441 ctctatttat atttatttag ttatactata gtttatattg ttattttatt ttcatatgca
    28501 gtatagggaa tggattacct tgttcatcga actcagattt tttaaagact ttaaatccca
    28561 tatgtttata aaagcctaat gcttgttgat tttgctcatt cacatctaca taatttacat
    28621 ttaatacttc tattgcatat tctacaagtc tttttccaat tccatttcct atactataat
    28681 tacttacaaa taacatttca attttaaaat catgtattcc cataaaagct tttataatac
    28741 ctttttttgt ctcttacaca taaaaaatta ctaacatatt tagcacattc tttaacttga
    28801 ggctttattg atattatatt ttcttcactt aaaaagtcat gtgttgctct tactgatgat
    28861 tcccgtgtat ctagtatttc tttcatatct ttattgttaa taatatttaa attttcaatt
    28921 tttatcattt aataattcct cttaagtaag tatgttactt aatattatat gttaaatatg
    28981 atttaaaaag gttataagaa tatttttgta ttaaatataa tcaaataaat atatttattt
    29041 gtcataaatt aaatgtccac taaaatagaa aaaataagaa gaaataggta ataaatgata
    29101 gcttatagca aaataaagat gtaagtataa caatagatga gattggaatt aagtcactat
    29161 acctaacaga acattcttag gcgaatatag aagacttaaa aaagaaaaga gaagtagaca
    29221 agaatttatt aatgattaca tataaaataa atgaattata attttattaa tgatataata
    29281 ataattaaca aaaaataaaa gaggaacgag ttaatgtttg atggcataca ctcattttct
    29341 aaccaataaa acaatagatt caattaaggt tactaaagtc taatcaacac ataaacatat
    29401 aggataaata catcttttta atatattaat tatgttagac ttacaaaaaa tgtaaataca
    29461 tagcttgtta aatcttgatt gaaccttaag taataaggga taaaaaatga cttgttcaaa
    29521 ggcaaagaat atactattaa atgatataag taacttagag ataatatgta gtaagtcaaa
    29581 agaactatat agcatcagta agtaaaaagc gtaaaaactt acataatgca ctataaaata
    29641 aatgagttag aaaataaata taatttatat aataaagtag tacaaaaagg agtatttatg
    29701 tttggatatg ttaaaataaa caaaatggat ttaacattta gagaatatga ttattacaaa
    29761 gcttactatt gtggactatg taaatactta aaaagaaatc atggagaaat ctctaggttt
    29821 tctttgaatt atgatataac atttttaatt gttttattaa cagcagtata taacccagag
    29881 tctataagta cagaggaagt gtgtatagta aatccattta aaaagaaaaa ggtaattaca
    29941 aatgatatta cagaatatgc agcaagtatg aatattcttt taacgtatta taaattggaa
    30001 gataatttaa tggatgataa acgcataaaa gataaattgg catattatat atataaaaac
    30061 aaattaaaat tagcatatga aaagtatcct gaaaaagcag agtatataaa acaacaacta
    30121 aatgaattaa ataagttaga aaaagataaa aatataaata tagatgaagt atctagtata
    30181 tttggaaata taatgggtga agtatttgtc tataaaaaag atgaaaatga gagaaactta
    30241 agaatgatag gttttaatat aggtaagtat atatatctac tagatgccta tgaagattta
    30301 gatgaagatt ttaagaaagg aagatataat ccatttatag agtatattga taaaaatgat
    30361 gagttaaaag aaaaagtaaa aaaaataata gtaacatcat taggattttt agctagggga
    30421 attgataatc taaatctaaa taaaaatgtt ggaataatag aaaatataat ttattctggt
    30481 gtttatttaa gatatattaa tatattagaa agtagaggtg gaaagaatgt acagtaatac
    30541 ttataataat gaaaatttta tgagagcaag agcattcata gaaaatagtg aatttaaaaa
    30601 agcatatgat tttttaaaaa ctttgactga caaatgtgca gagtggtatt atttaactgg
    30661 cttttctgca atgaacatag gatattatga agaaggtgaa gacttcttaa aaagagcaaa
    30721 atttatggag cctgaaaata gtgaatatag tgatgcactt agaagttata cacaatatag
    30781 aaatgattat agcaataggg ctgacaatta taatagaaga agacgtaatg atttagatgg
    30841 atgttgttgc tgttgctgtg atgattgctg ttgttgtcta ggtgacgatt gttgtgaaaa
    30901 ttgtgctaag ctttggtgtt tagattcatg ttgtgaatgt tttggtggag atttaataac
    30961 ttgttgttag gatgataaat atgagtataa aagttgctta tggaggagtt ttattagcat
    31021 taaatgtgat attgttgact cttacaaata ttattcctgt aaatacattg tttattatgg
    31081 ggttagcatc acttttagta tcaattgtaa taatggaatg gggctttaag agtggaatcg
    31141 ctttttatat aggttctata gttttaggat ttatagtgat ggcaagtaag tctcaatgga
    31201 ttgtatattc acttaccttt ggaatatatg gtattgtaaa atatcttata gaaaaagata
    31261 ggtctatata tattgagtat tttatgaaat tagtatttgc aaatataatg atacttatat
    31321 tgtatttttt attaagaaca atagtttata ttccaataaa catatttatt ataggttcat
    31381 ttgagatagc ctttatagta tatgactatg tttacagttc ttttataggt tattacaata
    31441 ataggttaag aaagatgcta tttaaaaaat aatagttgat ttttatattt aattttaatg
    31501 aaattgtagt ttggataagt gtaaatgata tatctgaata tacattaaag cataaagcta
    31561 aatttaatat attcttagtg caacatataa aagctttgat ttttagtcaa agcttttata
    31621 tgttagatat aaatattaga tataatataa gtttttaaaa tagatagtat aaattaaata
    31681 tccactatat ttacatctgt agatagagaa taaataatat cttttctttc tactattatc
    31741 tcaacatttt tagaaatttc ctcatcaagt gatatttgag ataaaagttc ctttgttgga
    31801 tttattgcta ttggatttcc aactcttttt agcatattta tatcaccatt agtatctcca
    31861 taggcatacg agtttgataa atctaaatca tactttacaa caaaatcatt tatagcatta
    31921 ttcttactta tagaatccca cattggaata actgtgcctg taaatatatt attttcaaag
    31981 acataatcac ttcctagaaa atcggttaca ttatattttt ttgccatttt ttcaacaaga
    32041 aatccagggc taccagatat aaatataact atgtgtcctt gatttagatg ccactcaatc
    32101 cttgaacgtg tgtatttata aactctatct gcttttaatt taattacttg gtcacttgta
    32161 aagttgatac aggttttatc taatcctttt agtgaatcaa cataaaggtc acatatttct
    32221 aatagataat catcataatt accttgtctt ttatcccaat ccataaaagt gtctctagca
    32281 tgtttatacc aatttttttg gtcaattata tcgtatttaa ttaatttttt aaaatgttca
    32341 accataagag agtctctata aagagtacca tctatatcaa aaaaagctgc tatatttttc
    32401 ataagaccac ctcaattaat catttcctta tattatatta taaattgtat ctaagttgtt
    32461 aaaatatatg tataataatt tatatattaa aaaacaagta ttttagagtt taaaggatgt
    32521 gactatatga attttaattt tgcaaaggct acaaattcta gacttatggg cagtttaggt
    32581 cttataataa attggataga tgatgaaaac aatcactttt gtcaatattt tttattagat
    32641 gcagagggat taggattagc agattatgtg agtttaaata acccaacaca agaagaagct
    32701 tatatggaag aagaaagatt gatgggagga tttggttcag atagagttga attaacgaag
    32761 gatgaatctt tatttttagt atcttatttt ggtaataaga atctttatta tgataagcta
    32821 ttgccaggag ataaatgtga gtatattgat attataaaaa actataaaac agatttaatt
    32881 atagaaaaat tatacaataa aatatgtaaa agagttgatg aggaagttga atttataaac
    32941 tatatgacta tgagatttat agcatgggat agagaaagtt taaaatattt ttctggaagt
    33001 gacgagattg caaatatgca tattactaat ataaatggaa cattacttaa aaacgtggta
    33061 agtgataaag gtcaaggcag atatataagt gaagctttat atgaagacaa tgatggatat
    33121 tatatatgta aaattgcttt ttgcataagt aagtgctatg aaactggttt tagaattaat
    33181 tctctattag tgacagataa agaacttatg tatgattttg aggtatttga tgagatttct
    33241 aaaagtgaat ttgtaagtgt ttacagtgta aattcacctg aagaatttgc aggagtattt
    33301 tatagagaaa atccatttct gttaaaaagt aatatgaatg aaggtatatt ttttactaga
    33361 tttaatttta ataatgacca tgtaaaagaa aatgtatata taataaacaa tgatatgaaa
    33421 gctatatatt acttaatggg aaataagttt tttattggta cttataacga aaatgattgc
    33481 aattatatca atgaaataag tcagtctaat tatagtgact atattaaatt tgaagagaaa
    33541 tttttctttg agcaaaatgc attatatgac tttgcagaat caggttcatt agattttgat
    33601 gattttttgg aataataaat ttagacatta tttaagcgag attaactgaa tttaatctcg
    33661 cttaaattta tttctataat attgggggaa tattatgagg aatacattta ttattgtggt
    33721 caattattaa aattttatac agtttattat aaaaaattta tacttttctt tatgttgttt
    33781 gccaatatta gtctcaaata cgagtaccat actattctga cccaccaaca caagaaagag
    33841 ttttttaaat gaaaaagtca gataaaatta aattttatct gactttttct atgtttatct
    33901 tattagatta tgttaatatc tgttgaccga ataagcgcat actcagtcaa ctttctatac
    33961 ctataataat attgggggaa tattatgagg aatacattta ttattatggt caattattaa
    34021 tgttttatac aacttgctca aaaaaatata tattacattt cttcctcatc aaattcttca
    34081 atattagtag cctgtggtat accttgtata gacatattac tagaaccata aggagtagga
    34141 tatatattag gaggcatagt actaggcata actacaggca taccttgcat tgtattatga
    34201 catataactc ctggtatcat cataagtaca ttaggaggca tattttgatt acatgagaag
    34261 ttaggaatac ccatagtatt attcatatta taccccatat tactgttcat gttattaggc
    34321 acattgttag gcatacccat gttactattc atattattag gcatattgtt aggcataccc
    34381 atgttactat tcatattatt aggcatattg ttaggcatac ccatgttact attcatgtta
    34441 ttaggcatat tgttaggcat acccatatta ctattcatat tgttagacat attgttaggc
    34501 atacccatgt tactattcat gttattaggc atattgttag gcatacccat attactattc
    34561 atattgttag acatattatt aggcataccc gtattattat tcatgttatt aggcatatta
    34621 tttgtagcag atgtattgta attcatattt ccggatgtat tatttgtgtt aggcaagttg
    34681 tttggagaat tactactttt aggataatga tttacattat taggatatag gttatttatg
    34741 ttctggttat taggtatttt tgtataacca ttagaaattt gagggtcatt tctcaattca
    34801 tcttggggaa tttcttcatt agtaatataa tctgcatagt tcgctgggtc taagtaaggc
    34861 atgtaactag tatctaaatc cttttttttc ttgttactca tattaaactt gcacctccta
    34921 gaaaactata aaaaagttta caaaaactta ttctaataat atatatatta aaaaagttta
    34981 taatatgtgc attctaatta tattaaatta ataaaaatta ttgttgcaaa tttaggaatg
    35041 atagcatata atgaacatat gaacagttat tcatatatta aaacaatgga ggattaaaga
    35101 tgagagaaga gataaatgat tgtaattgta atatagtaca tgaagaaata gtaacagagg
    35161 ctaaatcaac tatgccagat gaagagatgt tatatgattt agcagaatta tttaaagtat
    35221 ttggggatac tactagagta aagatattat atgcattatt tgcaaatgaa atgtgtgtat
    35281 gtgatatagc gagtttatta aatatgactc actctgcaat atctcatcaa cttagagtac
    35341 taaagcaggc tagattagta aagtttagaa gagaaggaaa aacagtgtat tactcattag
    35401 atgatagtca tataagccag atatttgatt gtggattaaa tcatattaga gaaacttata
    35461 aatgataaat gagaggagtg aatatatatg gaagcaaata atttaattaa aaaagaattt
    35521 atactaggag gattaaactg tgcacattgt gcagaggaga ttaataataa agtttctaaa
    35581 ctacaagaag ttaaatcttc caacttaaat tttataaata aaaaacttat agtaaatata
    35641 aaggaaagct ttaatgagga tactactata gaaaagataa tagatataat agattcaaca
    35701 gaaccaggtc ttgatataca aataagttct aaagaaaatg catctagtaa aacatcaatt
    35761 aaaaaagagc ttatattagg aggattaaac tgtgcacatt gtgcagagga gattaataac
    35821 aaggtttcta aattaaaaga agttgaatct tctaatttaa actttgtaaa taaaaaactt
    35881 acagtaaata taagtaataa ctttaaagag gatgatgtta taaataaaat aaaagaaata
    35941 ataaattcaa cagagccagg acttgatata caagtagggt ctactgacaa agtaaaaggg
    36001 agaactactg aaaaatcagg agctgtaaat gatacaaata agaaagaatt agttccactt
    36061 ataattggag cacttgtata tatatttggt atatatcaaa cagctacagg ttatgaaagt
    36121 cagtttagca atatagtatt tatagttgcc tatgtaatag taggtggcga tgtgctttta
    36181 agagcaataa gaaatatatc aaaaggaaga gtatttgatg agaacttttt gatggctcta
    36241 gcaacagtag gagcattaac aataggggag tcatcagaag cagtaggggt tatgcttttc
    36301 tataaagtag gagagtattt acaaggtgtt gcagttggta aatccagaaa gtctataact
    36361 tcacttatgc aaataagacc agattatgca aatctaaaat ttaattctga agttaaggta
    36421 gtgtcaccag aagaagtaaa tgtaggggat ataatagtag taaaacctgg tgaaaaagta
    36481 ccattagatg gtgtagtagt ggatggagtt tctatgctag atacttctgc actcacagga
    36541 gagtcagtgc tgagagaagt tgaaaaagga gatgaaatct tatcaggtgt tataaataaa
    36601 aatgctttat tgagtattga agttacaaag agttttggag aatcaacagt ttctaagatt
    36661 cttgaccttg ttgaaaattc tagcattaaa aaatcaaaga cagaaaactt tatatctaag
    36721 ttttcaagat actatactcc aatagtagta atagctgctt tattaatagc atttgtgcca
    36781 ccacttgtaa tctcaggtga ggtatttagt gattggttat atagaggatt gatattctta
    36841 gtcgtttctt gcccttgtgc attagtttta tcaataccac ttagtttctt tagtggaatt
    36901 gggtttgcat ctaaaaatgg tatccttatt aaaggaagta actatttaga agctttaaga
    36961 agtgtagata cagtagtatt tgataaaaca ggaacactta ctaaaggtgt gttcaatgta
    37021 actaagttaa atcctgaagg tatatcagaa gaagaattat tagaatatgc agctattgcg
    37081 gaggtaaatt caaatcatcc aatagcaaag tctatattaa gttactataa taaaaaaatt
    37141 gatttagata caatagacag ttatgaggaa atagcagctt atggaattag agtaaagcat
    37201 aatggcaatt ttatattagc aggtaatgag aaacttatga aaaaagaaaa tatatcttat
    37261 tcatcagcta aagaagtagg cacagtagtc tatattgctg ttgataaagt atatagaggt
    37321 tatatagtta tatctgatga agtaaaagaa gatagtaaaa atgccataag aagcttaaaa
    37381 gcaatagggg taaaagaagt tgtaatgtta actggagata atgagaaagt agcaaaaaat
    37441 attgctcaag aattagaatt ggatacagtt tactcaaatt tacttcctaa tgaaaaggta
    37501 gatagattag aagatttata tgaaggaaga actgaaaaag agaaaatagc atttgtaggt
    37561 gatggaataa atgatgctcc agtattagct cgtgctgatg ttggtatagc tatgggaggc
    37621 ttaggttcag atgctgctat tgaagctgca gatgtagtgc ttatgacaga tgagcctagc
    37681 aagatatcta aagcaattga aatagcaaac aagactaata agatagtgtg gcaaaacata
    37741 atatttgctc taggagtaaa aattatagtt atgatactag gtgctggagg agtagctact
    37801 atgtgggaag ctatatttgc tgatgtaggt gtggcactta tagcagtagt aaatgctatg
    37861 agagctatga gataagggat aaatgtaaat ccatactatt taaaaaactt ataattatat
    37921 attgaagtat gtaataatga aaggtatcaa taagtttctt ttaactttat tggtaccttt
    37981 ttattggagc gctttgcata tataaattta tattcattat ccattgtgtt tatttaatgt
    38041 atttatttag tgtagagctt ttatattgta attttaaaat ttagacatag aaggtttatt
    38101 aataagtcta tattaagttt aaattagatt cataaaaatt ataatactta tgattgtata
    38161 tatattatat taatagttat aatttttaca acaaaatgtg tgtgttaaaa tactctaaac
    38221 caacagtatg actgtcattc aataaattat gtaatataag aaagtaaata tttttaaaaa
    38281 ataatttaaa aaagtattga cgaaatattt ttaag
//
LOCUS       DAMKPG010000029        38095 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMKPG010000029 DAMKPG010000000
VERSION     DAMKPG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38095
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.031"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(66..2732)
                     /locus_tag="PC621_001593"
     CDS             complement(66..2732)
                     /locus_tag="PC621_001593"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF4108754.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(2808..3070)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3371..4198)
                     /locus_tag="PC621_001594"
     CDS             complement(3371..4198)
                     /locus_tag="PC621_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4108755.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(4246..5682)
                     /locus_tag="PC621_001595"
     CDS             complement(4246..5682)
                     /locus_tag="PC621_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF4108756.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(5769..6638)
                     /locus_tag="PC621_001596"
     CDS             complement(5769..6638)
                     /locus_tag="PC621_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF4108757.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(6796..7449)
                     /gene="phoU"
                     /locus_tag="PC621_001597"
     CDS             complement(6796..7449)
                     /gene="phoU"
                     /locus_tag="PC621_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF4108758.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(7477..8241)
                     /gene="pstB"
                     /locus_tag="PC621_001598"
     CDS             complement(7477..8241)
                     /gene="pstB"
                     /locus_tag="PC621_001598"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF4108759.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(8260..9084)
                     /gene="pstA"
                     /locus_tag="PC621_001599"
     CDS             complement(8260..9084)
                     /gene="pstA"
                     /locus_tag="PC621_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF4108760.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(9086..10003)
                     /gene="pstC"
                     /locus_tag="PC621_001600"
     CDS             complement(9086..10003)
                     /gene="pstC"
                     /locus_tag="PC621_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF4108761.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(10385..11620)
                     /locus_tag="PC621_001601"
     CDS             complement(10385..11620)
                     /locus_tag="PC621_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108762.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(11621..12508)
                     /locus_tag="PC621_001602"
     CDS             complement(11621..12508)
                     /locus_tag="PC621_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108763.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(12653..13768)
                     /locus_tag="PC621_001603"
     CDS             complement(12653..13768)
                     /locus_tag="PC621_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4108764.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(13804..14502)
                     /gene="vanR"
                     /locus_tag="PC621_001604"
     CDS             complement(13804..14502)
                     /gene="vanR"
                     /locus_tag="PC621_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF4108765.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(14884..15564)
                     /locus_tag="PC621_001605"
     CDS             complement(14884..15564)
                     /locus_tag="PC621_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108766.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(15614..17629)
                     /locus_tag="PC621_001606"
     CDS             complement(15614..17629)
                     /locus_tag="PC621_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF4108767.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(17701..18378)
                     /locus_tag="PC621_001607"
     CDS             complement(17701..18378)
                     /locus_tag="PC621_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108768.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(18958..19041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(19274..20116)
                     /locus_tag="PC621_001608"
     CDS             complement(19274..20116)
                     /locus_tag="PC621_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4108769.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(20368..22152)
                     /gene="pepF"
                     /locus_tag="PC621_001609"
     CDS             complement(20368..22152)
                     /gene="pepF"
                     /locus_tag="PC621_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF4108770.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(22218..22883)
                     /locus_tag="PC621_001610"
     CDS             complement(22218..22883)
                     /locus_tag="PC621_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF4108771.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(23034..23195)
                     /locus_tag="PC621_001611"
     CDS             complement(23034..23195)
                     /locus_tag="PC621_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF4108772.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(23273..23782)
                     /locus_tag="PC621_001612"
     CDS             complement(23273..23782)
                     /locus_tag="PC621_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF4108773.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            23988..24206
                     /locus_tag="PC621_001613"
     CDS             23988..24206
                     /locus_tag="PC621_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF4108774.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            24224..26170
                     /gene="feoB"
                     /locus_tag="PC621_001614"
     CDS             24224..26170
                     /gene="feoB"
                     /locus_tag="PC621_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF4108775.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(26330..27385)
                     /locus_tag="PC621_001615"
     CDS             complement(26330..27385)
                     /locus_tag="PC621_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF4108776.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(27433..28233)
                     /locus_tag="PC621_001616"
     CDS             complement(27433..28233)
                     /locus_tag="PC621_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF4108777.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(28226..29017)
                     /locus_tag="PC621_001617"
     CDS             complement(28226..29017)
                     /locus_tag="PC621_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4108778.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            complement(29057..29479)
                     /locus_tag="PC621_001618"
     CDS             complement(29057..29479)
                     /locus_tag="PC621_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF4108779.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            complement(29495..29974)
                     /locus_tag="PC621_001619"
     CDS             complement(29495..29974)
                     /locus_tag="PC621_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108780.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            complement(30106..32862)
                     /locus_tag="PC621_001620"
     CDS             complement(30106..32862)
                     /locus_tag="PC621_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108781.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            complement(33460..34263)
                     /gene="proC"
                     /locus_tag="PC621_001621"
     CDS             complement(33460..34263)
                     /gene="proC"
                     /locus_tag="PC621_001621"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF4108782.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(34588..36957)
                     /locus_tag="PC621_001622"
     CDS             complement(34588..36957)
                     /locus_tag="PC621_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF4108783.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(36962..37870)
                     /locus_tag="PC621_001623"
     CDS             complement(36962..37870)
                     /locus_tag="PC621_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF4108784.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT        12142 a         6756 c         4190 g        15007 t
ORIGIN      
        1 ataaagaaaa acatacaaaa agtatagaat atatactttt tgtatgtttt tactaattct
       61 tatatttatt ttattttaga ttctaaattt gttaatcttt cctcaacaga tttaatcatc
      121 tcttcaaatt tttctttttt tactttttct tcttcaatta aactttcagg agcttttgct
      181 aagaaacctt gatttgcaag ctttccatta actctcttta tttcgccttc taactttttc
      241 ttttctttag acaatctctc aatttctttt tcaaaatcaa ctaattcatt aagaggtata
      301 aatatttcaa caccatcaat aactactcca actgcatctt ctggaacatt agatttatct
      361 tttgctatct ctacatttga agcagatgct agagttataa aatagtcttt accaagctca
      421 atagcttcta ttttttcttc acttgggatt attatcaatt tagctttctt agaaggtggt
      481 acattcattt ctgctctcac atttcttata cttcttatac cttccatcaa taagttcatc
      541 atatcttctt cttcagccat gttatcttct tcattatatt ttggccattc agatactatt
      601 atacatcctt caacagtagg aagatgagta tatatttcct ctgttataaa tggcatgtat
      661 ggatgtaata attttaatat tttttcaaga acataagtta atgtatataa tgctgcagat
      721 ttagcatttg catcttcccc atataatcta ggtttaacca tttcaatata ccagtcacag
      781 tattctgacc aagtaaaatc gtatatcttt tgaagtgcta ttcctaaatc aaacttatcc
      841 atgttattag ttgcttcttt tacaatatta tttgctcttg atattatcca tttatcagct
      901 agagttaagt cctctttaac actttctcta gtcatgtttt taattatatc ttcatcaata
      961 ttcataaata caaatcttga tgcattccac aacttatttg caaagtttct agcaaattct
     1021 actctttcca tatagaatct catatcattt cctggggaat ttccagttgt taatgtaaat
     1081 cttagggcat cagctccata ttgctcgatt atctccaatg ggtctatacc atttcctaaa
     1141 gatttactca tttttctacc ttgagaatct cttactaatc catgaaccaa aacatggtca
     1201 aatggctttt cattcataca gaacatacct gcaaatgcca ttctaactac ccagaagaat
     1261 attatatcgt atcctgtaac aagtacactt gttggataat aataatctaa agcttcagtc
     1321 ttatttggcc atcctagagt agagaaaggc cataatgcag aactgaacca agtatctaag
     1381 gcatcttcat cttgtttaaa atgagtgctt ccacatttag gacacttatc tggcattttt
     1441 ctagcaacta ctatttctcc acactcttga caataataag ctggtatttg atgaccccac
     1501 cataattgtc ttgatataca ccaatctctt atattttcta gccattgtaa ataagtttta
     1561 tcaaatttat ctggaacaaa ttctaattct ccttttttaa gaatatctat agctggtttt
     1621 gcaagttctt ccatcttaac aaaccattgc tctgataatc taggctcaac tactgtatgg
     1681 catctataac aagttcctac attatgatta tgcgctttta tcttaattag gtaaccttgt
     1741 tcttttaagt ctgctacgat agcttttcta cactcaaatc tatccatacc ttcatatttt
     1801 ccacaaactt cattcatagt tccatcttca ttcatagtat ttaactgctc aagattatgt
     1861 cttaatccta cttcaaaatc attagggtca tgtgctggag tcatcttaac tgcaccagtt
     1921 ccaaactcta aatcaacata actatctgca actattggaa gttctcttcc tactaatgga
     1981 agtattgctg tttttcctat taaatgttta tatctatcat cttctggatt tactgctata
     2041 ccagtatctc ctatcattgt ctcaggtcta gttgtagcta tttctaaaaa gtcttcacta
     2101 tcttttagtg ggtactttat atgataaaag tttccgtcgt gttcttcatg ttcaacttct
     2161 gcatctgata aagtagtttt gcaatctggg caccagttta ttattctatt tcctctgtat
     2221 atgtgacctt tttcatatag gcttacaaaa aattctataa ctgcttcatt acacccttca
     2281 tccatagtaa atctttcttt atcccaatca caagaatcac ctagttgttt aagctgatta
     2341 gatatttttc ctccaaattc atctttccat tcccaggctc tctttaaaaa ttcttctcta
     2401 cctatttcat actttgtctt gccttcttgt ttttttattc tttcaacaac tttaacttct
     2461 gttgctatag aagcatggtc agttcctgga agccaaaaag cttcatatcc atccatacgt
     2521 ttccatctga ttaatatatc ttgaagtgta tggtcaagag cgtgtcccat atgcaattgc
     2581 cctgttatat ttggtggtgg catcattatt gtaaatggtt ttttatctgg atttggttta
     2641 gatttaaaat acccttcatc catccatttt ttatatagcc tagcctcaaa atcttttggg
     2701 ttataagttt ttgataaatt cgtattttcc attgtcctgc ctcctgtatt ttataaataa
     2761 attttttaaa tatctttaga aataaaaaag agctcttcat cctctttaaa aaggacgaaa
     2821 agctcgcgtt accaccttaa ttccaaagct taaaatacat taacatataa ttaaattatt
     2881 agttaatgtt attttatcaa ctttgactct caaagaattt aacggttcta accgtctaaa
     2941 cttacttata gttcagttta gaaactccaa agctacattc agtaaatctt atacttagaa
     3001 aatcttacag ccagtgaatt ttcctctctt ttagtagtat ttacttactc ctcttcttct
     3061 cagtctttaa tattacatat tattatatac aaatattatt ctctttgtca attattttta
     3121 ttttattttt tctgaatttt cttaattatg tctattctct tttatatagt tgttatattt
     3181 taaaatatgt ttttacagtt attctgtctt ataatgtcaa taattgcaag attattttat
     3241 tttaatttta atgctttctc attaatttta tattatatta tccttacagt atacctctta
     3301 ttcgtatttt ttatattata tgttttattt tataaataat ttactttatc ttttactata
     3361 tttaaattta ttataagttt tcttgccaga aatttttagg tgttatattt tctgtaattg
     3421 gcaatattgt atatcctctc tcttttaaga ttcttataat attatttaaa gcatttaaac
     3481 tttgctcttt ctcatgaatt aatactacta attcatctct ttttcttcca taatataata
     3541 tattacttac tatctgactt gtagaagact tccaatcctg tgtatccaaa ttccagtccc
     3601 aaattaaata atcatttgcc ataagtttat tatatgaacc ttctggcata tatggtttac
     3661 ttccgtatgg taatcttatt aattttgaac tctgaccagt tatttcttgt agagtattat
     3721 tgcatgtatt aaattcccca actgttactt ctgggctttt atataactta tgtatatcat
     3781 gacttacact atgaaaacct attgagtttt cctcttccaa gactctcctt acttcttcct
     3841 tgtgattttt catattttga ttaagcataa aaaatgtcgc tttgacatca ttttctttta
     3901 aaatatcaag tagtgcattt gtatattttg atggaccatc atctattgtt atgtatgcta
     3961 ctttttcaga agtagcccct ggaccttttg tttcttcttg agatgctttg tatatgattt
     4021 ctcctaaagt attactttga gaaagcagtt ccagtgtatt ttcacctttt gaacttatat
     4081 tttcaactgg aacattatta attttttcct ccaataatgt tccagttaaa attaaaacag
     4141 ctataagaaa tatccttata aatacatgaa aaacagcctt attatcttta attttcatcc
     4201 cccatatctc ccttttataa aagcataagt ttaagactta aaaagttatt ttttatattt
     4261 attctctttc ttattacttt ttatatctct actaaatatt tttttaacct tttcttcttc
     4321 tttttctcct aaagattcta ctcttttgat tcccaaagaa gttaaaaatt gtaatacatt
     4381 taaattatca aatggagttt ccatattcgt actttcacta tattcatcaa cattcttagt
     4441 accttcttcc acacttaaaa tacgcttcgg acctcctaca cagcctccta cacaccccat
     4501 tccttctata aatgtagctt ctatttcttt atttagaact ttctttagac cgtccatgca
     4561 ctcttttgtt ccttcaaatg ccttttcttc aaattttata tttttgtcaa ttcttctgac
     4621 tgataactta actgctttac ttacacctcc tgttctagca taaactctac ctgaaaggga
     4681 cgcctctact ctattactct catccattat tgctggattt aactctaatg catcaaatat
     4741 ttcttgcaac tctttaaatg taagcacaaa atcaactgca tcttttatat cttctaacat
     4801 tgcttctttc ttttttgcca tacaaggacc tatgaataca actttagcat ctggatttaa
     4861 aatttttaca gctctcccac aagcaatcat aggagaaacg gatggtgata cattctccaa
     4921 tatctgagga aaactatttt gtatcaaact aacccaaata ggacagcaac aactagttat
     4981 aaagcatcct tcttcgtcct ttttcatatg ctcataataa tcatatgctt cttttgcagt
     5041 aagcatatct gctgccaaag caacttctat catatcttca aatcctatct tttttaatgc
     5101 agttcttaat ttaccagggc ttacatcttt cccaaactgt cctacaaaag ctggtgcaac
     5161 tattgcatat acagggctct ttatatcttt taacaatttt atcattggta taaattctat
     5221 tttatctgaa attgcaccca acttacatct tgatacacaa tcaccacaag cactacatac
     5281 atcttcctca ttacaattaa tatgcttact tcttatctta catgactcat cttccttatt
     5341 attttcatca caatatttgc aagcatctga tattacagat attattggtc ttttgacttc
     5401 atctaaattt acagcttcat gtagtgactc ttttaaattt ttatctgtat cttctgtcac
     5461 atccaatccc atagttaccc taattaaatt taccatagta tctttatcaa attttccact
     5521 aaaataatta tttaaattat cctttaaatc ttcatttaaa attttattag gtaagttttc
     5581 aatatcctct atatcatcat tccacactgc ttttacaagt tcgccaaaaa gcttcatttt
     5641 atatatttct agtattttag aattgtcatt tctagtattc ataaaaaata gtacctccat
     5701 aaattaaact tatatatgtt taatatttac aaagatacta ttttttattt attatttaac
     5761 tactattatt aatatgattt atttagcttt ctcaacaact ctatttcaat cattattttc
     5821 attagatgat agttgaatac ggggcttttt tcttcatcta tattccagtc tagcttatcc
     5881 acattatatg ttttcttaag gtatttatat gtatgctctt tcaaataaag tttattttca
     5941 agtaactcta tagactgcat atgaaaatgt atttctctac atatccttaa ggcattctca
     6001 attttttcta tattagtttc actcaaacta taatccaact catcaataag ttttgaatat
     6061 atttcttcta atttttctac ttcttcttca aatttctcta catttaaatt ttcttttcta
     6121 agaattttat cttcaataag taacattgaa agttcttcta tctttttaaa ttcatctata
     6181 acactattta gataatttgg tctagccaca taataattaa caagtactcc aaacaaaaca
     6241 cctacagatg tatctaacac tctatgtaaa gaataataca cagggtcact atttcctaca
     6301 cctagatgta tagccagaaa ggttacacag gcaactacta ctgactttat tttaaacaga
     6361 ttgcataaat atattgagca cattactcct agaccacata aaaaagggtc tccagcttta
     6421 atgatggcaa atatgaatcc tattatacca cctaaaatag taccttccac tctattgatt
     6481 cctgtcttta aagaaccttt taccgtatct tgaacagaaa ttatacatgc tactagagaa
     6541 taaaacatat tctctactag atagttacca agaatcatac ataaagtgac actaattcct
     6601 gtttttatag tcctcatacc catcttttga agtttcaaag ttccacctcc taaaaagcac
     6661 cttatataaa atacgcatcc tcatatttaa tatgttccta gtatatcaca attttttaaa
     6721 tttacccatt aatcatattt ttttatcaat tctttcttaa tttagacaat aaaaaaactg
     6781 ctttaaagca gttttttatc ctatctcaac cattttaccc tcaattgcaa aaattatctt
     6841 ctcacataca tttgttatat ggtctccaat tctttcaagg tatcttccta taagtagaag
     6901 ttttactcct tggtctatag tatttgaatt ttcatgcatt atttttaaac aatcaatttg
     6961 tacttgttca tacaatccat ctacaatatc atctttagtt ccaacatcat aagctatact
     7021 tgcatcaaat aagattaaag cttctctagt ctgagacatc atagacttac attcttttgc
     7081 catcttagga atatctatca gctcttttat ataagcctct ccacctatct tcaacatttc
     7141 tctagctata tttgctgcat aatctcctat tctttccaac tctatcgata tattacctaa
     7201 tgaataaata aatcttaagt cccttgccat tggttgcttt aatactatta gctctatact
     7261 cttatctctt atttcttctc ttaatttatt tatttcttca tcatcttcta ctacttttct
     7321 tgcaccctct aaatccttct ctatcataca agtcacagac ttttctattg cttcttcaca
     7381 tttttccatc ataagtactg tttgttcttt taaagtatca atacttatat ctaaactatt
     7441 attgaccatt gtctaactcc tttatagtta tatattttaa ccaaatctac ctgttatata
     7501 gtcttctgtt cttttatcta caggagtggt aaacatagtt tttgtatcgc taaattctat
     7561 aacctctcca tttaagaaaa aagctgtttc atctgatata cgagcagctt gttgcatatt
     7621 atgtgtaact atgactattg tgtagtcttt ttttaaatcc tctataagtt cctctacttt
     7681 taatgttgaa attgggtcta aagcagatgt aggttcatcc ataagaataa cttctggtct
     7741 catagcaatt gctcttgcta tacatatacg ctgttgttga ccaccagaaa gtcctagtgc
     7801 agatgattta agcctgtctt taacctcatc ccaaatagct gcacctttta agctttcttc
     7861 tacaatttta tctaattgct tcttatctct tagtccatgt gtacgaggac catatgccac
     7921 attatcatat atactcatag gaaatggatt tggtttttga aataccattc ctactttagt
     7981 acgaagattt ataacatcat ctgaggtata tatgtcctct ccatcaacag aaatgtttcc
     8041 tttaattgtt acatcttcga ttaagtcatt cattctattt aacgttctta taaaagttga
     8101 ttttccgcat ccagatggac ctattaaagc agtaacctta ttctctttta tatccatatt
     8161 tattttcttt aatgcttgct tatcaccata aaataaatcc aaatctttta cactcatttt
     8221 tatcttatcg attaattcca ttattcacac ctcttaaatt taataattag ctttatttaa
     8281 tttcttagca acaaatcttg ctagcatgtt tagtgctaat acaattacta ttaaaacaat
     8341 tcctatactg gctgctgttg ctatatctcc actctcttta gtcagtaagt atgagtgtac
     8401 agttaatgtt cttgcactat caaatattcc acctggcatc ttggctacag tacctgctgt
     8461 aagtaatatt gcagctgatt ctccaattat acgaccaact gataagatta ctccagataa
     8521 gattcctggt attgcacttg gaagtactac cttatataaa gtttgaaatt ttgtagctcc
     8581 taatgccaat gatgattctc tataagcttg aggaactgtt tttaaagctt cttctgttgt
     8641 acgaattatt actggtaata ttattattgc aactgtaagt gaacctgcaa ctatggaata
     8701 tcccattttt aatgtaacta caaagaaaat tcctccaaat aatccatata ctatagatgg
     8761 tatccctgat aaactttcag ttgcaaatct tatagctgta actgcttttc cttttttagc
     8821 atattcttgt aaatatatag cagccaatat tcctatagga gttgctacaa ctatagaaac
     8881 tactactgta tataatgttg taattatcat tggaagtata ccaccatcac ctgatgctga
     8941 ataattgcta aataagaaat taaaattcat gttgccaatt ccctttataa atataaatcc
     9001 cactataatg actaatgata ctattgtaaa tagtgctgat agatatacta atgacttcaa
     9061 tagattctct ttgaactttc tcatcttaat ttaccgcctt atttgaaagt ttatttaaaa
     9121 ctaagtttaa aattaatata aatgtaaaca atactactcc agttgcaaat aacatttctt
     9181 gatgtgtacc aaatgcgtat cccatttcaa gtgctatatt agttgtaagt ggtcttacac
     9241 tatctgttat tgcagttggc ataactggtg agtttcctgc aacaagtatt actgccattg
     9301 tttcacctaa agctcttcct actcctaata ctactgcagc taatactcca gatttcgcag
     9361 caggtagtac aactttgaat attgtttcta tttttgatgc accaagtgct aatgaacctt
     9421 ctttgtatgc ttttggtact gctcttattg ctgtttctga tacagatatt attgttggta
     9481 acatcattac tgctaatact attactattg ctaacaaact ttgacctttt ggaagattaa
     9541 atatactttg tatagttgga actatcactg caagaccaaa tacaccatat aatactgaag
     9601 gtatacctgc taaaagttca actgctggtg acattatttt tgctaatctt ttaggtgcta
     9661 cttctgctat aaatacagag gttaatatac caacaggtac tcctatcaca agtgctccaa
     9721 gtgttgcaaa tattgatgct actatcatag atgatattcc aaatttattg gcagaaggaa
     9781 cccaatctgc accagttaaa aagtctataa atgaatatcc ttcaaatata aatggtctta
     9841 agcctttata aaatacgaat cctattatca aaagtaaact tgcaactgct actaaagcac
     9901 taagtagaaa aatatttttt gctacttttt caattacgta ttttgtctta ttgccattat
     9961 tgtcattttt ttctaaacct actaatctag attttactat cataataccc tcccattaaa
    10021 atatacttat cgattttcgt tctgtattca aaatttaatt ttttataatt cgtcttcatc
    10081 aaatttcctg tacttttatt ctactttttt taagttaaat ctatattagg attaagttaa
    10141 gcaagtgtaa aattatattt acttttttaa cattaatttt acttttaaaa ctatatttga
    10201 catttacttt acaatatact gttgtttctc tatattaaaa tagcagatat agataataat
    10261 acagtttatg tataatttat tacctatatc tgctattaag atttgttaac taaatttaac
    10321 ttttatacta catatataat tatcgtatcc tctagtttat atcactatta tttctatcaa
    10381 attgttatga tacttgatgt ttgcaatagg tacgaatagc caaaaatata aaaataggta
    10441 tttctatcac tgagaaaaac agtataatgt gtggtgtcca aaaactcata cctcctaagt
    10501 gtaagaaatt aaatgatgtc atttgatata ataaactaat cttaagacca attccccctg
    10561 gtggtaatag gtatccaatc caactaacac ctattcccat aggaattaag caaatagaaa
    10621 aagcaatcag catagaagta agagaatcct tacattttgc agataaaaat aatgttaaac
    10681 taaccattgc aagtattgaa attagaccac caatagctat aagaatctgt gcttgtagta
    10741 aatttatatt tactagacta atgacagaaa acaacatttg aaatgaggtt ttcaaacatt
    10801 cagtaccaaa agctaaatca gaaattgcca aatggataga aatacataca acaaacatta
    10861 ctataaatat agaaaataga gctaatattt tagtaaacgc tagacgagta tgtccatgtt
    10921 tcgtacaacg aaggatgcta tctgattgtg tctgatactc accagaaaaa gtaggtgcag
    10981 taattgcaac acatataatt aataatataa aaatgtaaaa tattatataa tcgaaagcat
    11041 ctttagaata acctgggtat atctcaaatg gagtatctac ttttctataa agttcttgag
    11101 ctttcatttg tgcactggaa gtttgtcgtt caatcttcat aatatcattt aaatgagttt
    11161 tgcattgttc ataaaaagaa tctaaatcat ttgtatttat ttctgataat tctgttgcaa
    11221 atcctgtttc tgaattagca aatgcttcag gaagtctatt caacaatggg cgtattggta
    11281 aaatactttt agaataaata tctagtggga aatcttcact acctacttta ccatactttt
    11341 cttctgtttt tttccatata tttaaagcat gctttaattt ttttggtgtt acaactccac
    11401 tactaatttc actattatta gcataaattg tttttttata tttaatagca tctaaaccac
    11461 tcaattcaac taccttgcca ttattatcta aataattgat accctcgtaa gtaattggaa
    11521 gaaaagccat caatgcagat aaaaccaatg ccaacaataa taaaattaat gtacgacgtg
    11581 agtgcaacac tcgtttaatt tccaaataaa aaatacgcat ttatttcccc tccttactat
    11641 aaactgcttc tggaaataac cataaatata aatcttctag tcgtggttca acagcaatag
    11701 agttatcaat ttgtgacttt tctgccaagt aacgaataga tacttgattg tttttttcac
    11761 tacgctgatt gattatacgt aaattcattt catattttga aatatctcta tttgatataa
    11821 gacattccca tactttgccc tctactttct taaccagttt atcggttgtt cccacatcaa
    11881 gaatttttcc acttttcata atagcatttt gagttgatat gtactcaata tcagatacaa
    11941 tgtgagtaga tattaggaca acacgattat gagaaaattc tgaaataaaa ttacgaaatc
    12001 tcactcgctc accagggtca agccctgctg ttggttcatc cattataagg attttagggt
    12061 catttaacat ggcttgtgca attcccactc gtcgtttcat tccaccagat aatttaataa
    12121 tctttttatt ttttacatca gataatgtaa gaatttccaa tagattatta atttttcctt
    12181 ttacttctct tggtggtaca tctttcaatg ctgctatata ttctaaataa tcttttacag
    12241 aaaaatcctg tgggaaacca aattcttgtg gtaaaaatcc cagtaaattt ctgtactcag
    12301 cacccattat ataaatattt tttccattat acagaatttt gccattggta ggtttcataa
    12361 tgtctgtaat aattctcatc aaggtagttt tacctgctcc gtttgctcct agaaatcccc
    12421 acacacctgg tgttaaggta aggcaaacat cttctacagc aattttatcc ttaaattttt
    12481 ttgtcacatg ttcaattttt aattccatat ttgaaactcc tctctttatc tacttacctg
    12541 caaaaacaaa agtgtcttgc ttagtattta ttttactaaa caaagacact cataatttta
    12601 atattaaata gataatttct taagaaaatc ctaatatttg atttatattt tactaaatag
    12661 atgtaggaag cgatatagta aatgttgtga cttcatcttt actttgaaca gtaatatcac
    12721 caccatgcaa atttataatt tccttagcta tagaaagccc taaacctgca ccacccatat
    12781 cagattttct agcttcatct aaacgattga acttttcaaa tatagcagaa agttgttctt
    12841 ctggaattgt ctgtccataa tttctaaaag caatatctac cttgctgtct ttatgaaccg
    12901 ctgagataat aatttctgta ttaggatagc tgtaagcaat agcatttttc aagatattat
    12961 taaacactct tgcaagtttt tcagaatctg cttttattgt taaactttca tttgctttta
    13021 gaatggctct atttcctttt tcagacagca atgggtaaaa ttcatccttt aattgaagta
    13081 acatataata taggtctact ttttcgaaat taattttgat ttgattagaa ttatatcgtg
    13141 ttatttcaaa aagttcattg ataagttttt ctaaatattg tgctttgtca agtgcaactt
    13201 cagtatactt agatttttga gaattatcca tattaggtat ttcttttaat aagctcaaat
    13261 acccaataac tgaagtaagt ggtgtcctta tatcatgtgc tagatacatc acaaggttat
    13321 tctttttatt ttcagcctct ttcactgctt gttgacttaa caaaacagac atcttaattt
    13381 ggttcatttg ttgctctatt tcctttaatg gcttagatag ttcaaccaat tcatcatttt
    13441 gttgataaat agtatgtgtt gcattgatga tttctcttaa ataattccat ggttttttcc
    13501 aataaaaatt gaaaatagtg atatatccaa ttataaaaac caaaaaaaat aatacatctc
    13561 ttctcaactt aagccattta acaagaggat ttgaaaaatt tgaaaacgta taatcagcca
    13621 taaacattaa aaccaatact atacctgtat agatagctaa cattatatat aatttatact
    13681 ttaaatacgc ctctgtattt ttttctcttt ttataaattt atatttatct cttagtttcc
    13741 tataagtaaa attattaatc tttttcataa tactacatcc cttcttgaca agaaaaatag
    13801 gtgtcaaatc ttgtaaccaa caccccaaat tgtttttata tattttgctt catctacagt
    13861 atcacctaat ttttcacgaa gatggcgaat atgaactgtt atagtattgt tacttttatt
    13921 gtaatattca tctttccaga tagcatgaaa aagattttct gaatttacca cagagccttt
    13981 atttttcaat aatacatgta gaatagaaaa ctctgttgga gtaagtgaaa gagctttacc
    14041 atttaaatgg cattcatatg tttgggtatt caattccaga cctaaaaaag aaaaaatatg
    14101 tgaaagttct tcaccagatt gtaaacttcc actgtatttc ttatatcttc taatctgtgc
    14161 cttcactcta gcaacaagtt ccaaaggtcg gaaaggttta gtaacataat catctgcacc
    14221 taaagttaag cctgtaatct tgtctgtttc ttcatcctta gctgttaaca taatgattgg
    14281 ataagtatat ttttctcgga ttttttggca taaagaaaat ccatttactc ctggcaacat
    14341 aacatctaaa attgcaaggt caaattcatt taaattaatg cagtttagtg catcaagacc
    14401 agaataaaat ttataaacaa tatagttttc atttgtaagg tagacttcta ttaaatcagc
    14461 tatatcacgt tcgtcatcta caactaaaat ttttccactc atagacttta aacatccttt
    14521 ctatatatat ttatatatat tatactaaat ttaatttttt tatatgattt tttacattag
    14581 aatttattaa gaaattctta atcttattat gtaactattt tattatttaa catatattaa
    14641 agataaaaat gaatatatat agatttagat atacaattta tctatctttt caaatataat
    14701 ctataaaata atagttctgt tacgcatttt ttcatataat aaaactgact gtaatttaac
    14761 tggcaaagtc tagaagaata ggctttatca attaaattac agtcagctat tttataataa
    14821 tttagtttag atatcgttag catttcacat ttataacaat tattaatgag attaatatat
    14881 ttattattct ttattaaaat aatatcctgc tcctcttatt gttataattt tctgaggatt
    14941 tttataatct ttctctattt tctttcttaa tccattcact gcaactacca cactatagtt
    15001 atcctctaag tactcattca tccatacatg ttcatatatc tctttagtag tcagagtctt
    15061 ttcaggattc tctataaaaa agtttaacag cttattttct gttggagtca attttattat
    15121 tttgtcttct aactttatta ctccactagt tctattatat tcctctaatt ttgtattatt
    15181 ttcattatta tttacatatg agttcattct tcttaaaata gcttttactt ttgcatataa
    15241 tatttctaaa tttagaggct ttattaagta atcatctccg ccagaattta aagcatttaa
    15301 aatgctttgt tcttcattta tataagtcat ataaacaatt ggacaagtag ttacatttct
    15361 aattttttta caaagagtcc atccatcacc atcagttaga attatttcta aaaatattaa
    15421 atcatattta ttgctaaata tttttccaat agcttcttca caattatatg ctaaatctat
    15481 acttatttct ttttcctcca gaaatccttt tagcttctgg caattatcct tgtctccatc
    15541 tattatcaat atcttagttt gcattataat cactcccaaa tatcattatt cttttatatt
    15601 aaataagcta ttattataaa aaacatagac ttaattttat tgtaaaaaca gttccttttc
    15661 ctagttctga atctacaaag atttctccgc catgctctct tataatggat ttgacaatat
    15721 ataaccctag cccttgccct tttggctcat tattatctct ttctgtaaag aatctgtcaa
    15781 atacctttgg tatattttct ggaggtatac cttttccatt atcagaaact ttaataattg
    15841 caaatccatc ttctttatcc attgataaaa ttatctttcc atcaaatgga gtaaatgtca
    15901 aagcattata aattaaattc tcaaatgctc ttgtaagcat ctctttattt atctttgcat
    15961 atattttttt ctccaatatg taagtttcaa agtttacacc acatgcttgt gtatctggca
    16021 agttattttc atatatctct ttaataaaat catttagaca tattttttca ctaataccaa
    16081 tgcttttatc ttgtgatgta aattcttgca aactacgtac tctattttgt acttcatttg
    16141 acttttgatt tatagcataa agataggttc ttgtctcttc atctacatga atattaccaa
    16201 cctttatcaa gtctataaat gattgtatag ctgaaacagg tgcctttaaa tcatgtgcca
    16261 catctgccaa gaatttttta cgttcattta acaatgttgt aagttcatta gttctcactt
    16321 caacttcatg ttcaagattc cttgtaagtt tttcattctc ttttattatt attctattcc
    16381 tgtttatcat cataagagaa aataaaataa caattataaa tccacaatat tctgtttgcc
    16441 accctgtaaa tataggctca aatctattac tagtaactgc atcaaataat atgctaattc
    16501 caaaaacagt gtttactgaa actaaaatat aagaacttat atgctcagag catgtctttt
    16561 tatatattga aaccagcgaa gacattaata tatatataaa tacaatatat ttatatatgt
    16621 ctattaattt cccatatatg ttgacaaagt acatatcata tggaagaaga ataattggta
    16681 tcactataga tatgatactc attgtaattc ctaatggtaa aattaaaaat ctatacactt
    16741 tatcttcttc tattttggat atacttccat ttatacaaat aatacataaa actaccataa
    16801 aataggacga atcttcaacc gcatatgtaa agcttaccaa aggcagtcct aacaaatgta
    16861 caaagggata ggatacatgg atggcaaaga aaacacacat acatccaaaa tataagtaaa
    16921 gtttactttt gtttgaaaga aaccatacag acaacgaaaa tatagctatt gcaaaagttg
    16981 tgaagcaaat tatactataa aaaagtaatc gataaaacat catattactt atgtcctttg
    17041 tttctcctaa tgcaggcgga taatacaaac cactataata atgtgaaaaa ttcgatacat
    17101 tgattataat ttctgtatct ttctttgcaa taaaacttac tactgaattt ttaattttgg
    17161 gtctatattt ttctgttcct acatctccta atttagaaac tatttctcca tttatccata
    17221 cttcacttgc ggaaaaaatc tcaggaagct ctaaacttag tagcttatcc tcaccttcat
    17281 tttttattct catacgataa gtagcattcc catatggtga ctgattccct tttctcatag
    17341 aaaaatctgg atattgacct ataaaaacat attctggttt tatatcttgc tctgtaaaat
    17401 cattcggttt aaatatttta tgattataaa actcccagtc atctatcaga aagacaagtt
    17461 tacctttagt gaaagtatca ttatctatag ttaaaattcc attacttgct ctaatagact
    17521 tatatgtata tttattatca tatttataca gaagatttaa tcctgctatt gccaacagag
    17581 agactatcaa tacaatgaga gttcctttta gtatattata ctttttcatt gcatcaacca
    17641 catctctttc atagtagtat taaataaaac aagaactatt tattaattac agtatctcac
    17701 ttatggtaca aacttatatc cttttcccca tacggtttga atgtattgat gctccctgct
    17761 taaagaatta attttttttc tcatctgtgc aatatgaacc tgcactgtat gttgatctcc
    17821 aatatcaggc attttccaaa cttggttata tatctctatc ttcgaaaata cttgttcttt
    17881 gttttcagct agaaacaata atatttcaaa ttccatagta gttaaatcta tactttctcc
    17941 atcaaaagtt acttttcttg aatttacatt tattactaac gctgaaaact ttaacacatt
    18001 aggaactgat gtaatatttt tgtactgcct catacgagca tatattctaa gttcaagttc
    18061 ttttaaacta taaggttttg ttatataatc atccccccca gctaaaaatc cccgaacttt
    18121 ttcttcttca tgctcataat tagataaaaa tattataggt atgttaacct tttcttttat
    18181 atcattgcac attttatatc catctatatc tggcaatgaa atatccaaaa ttatacagtc
    18241 taaaactata cgctctacta tatccatagc atctttagca gtctctgatg tatatacatt
    18301 aaaacctata ttagaaaaat aaatttgatt tatatttaaa atatcaacat catcatctac
    18361 aaccagtaaa ttatacatta caccactcca ttcaaaggta ttttaatcat aatctcaagt
    18421 tttataatta ttacaaatta taatgtttta ttttcatata taactagtat tattatttta
    18481 taatatcttt acaaactaga ctataatact taagtatttt tgaacaattc ttgaatattg
    18541 tattgaacat taagatttat tattaaatac taattaaagt ctccgattgt tggagaatgg
    18601 agcttaaagt cattttcact tataaattta ttatttttct agaaacttgt ctattggcta
    18661 gtttgagtat tgatttatat gatttatatg gttataatat aaaataaatt gtattatttt
    18721 ggtaaatata ttgttacaaa aggtttatat tttgcatctc aatgtttata aaaactttat
    18781 tttcttatta taatagtatt atacttttta aaaaatttcc cttttccaac aaatggctac
    18841 tttaaaagcc aaaatttcac ctatcaataa aaatcttaac gactaatact cttattttca
    18901 aattttgcaa catttttgtt aattttaaag caaaaaaaga ctatgcaata taaaaattat
    18961 attcatagcc ggttgcacca ctaactccat atatctccag atgcccaata ataagatata
    19021 cttctcagtt tctaaaatat ttttattaaa aatcttttat atattttcat aaatttcttt
    19081 tttattctaa cattgtaata ttattttgtc aatatttcca tatttttaga tataagatac
    19141 taaaatacct attttatcta aagatattta tatttctaca ttcaataata gacaataccc
    19201 tgcaagttaa agcatcattt ttatgagctt atctcacagg gtattatatg tttactttat
    19261 attataattc atgttattga ttaactttaa taaatccttt ttctgaaaca agtgcttgtc
    19321 cttcatcact taatatataa tctattaaag ctttcccttt ttctgacaaa ccatcttctt
    19381 tattaactaa tataaatggt cttgatattg gatatttatt agccattaca tttgcatcag
    19441 ttgcttcaac atcatttatt tttaaactat ttactttctc atcaacatat ccaaatgata
    19501 tatatcctat agcattttca tttccttcaa ctgtagtttt tatatttcct gaaccatcac
    19561 ttatttgtgc atctttagtt agttcttctg aatcaaaccc tacaatttct tggaatccat
    19621 ctctagttcc tgaaccttct tctcttgata ctattactat tggtgcatca tttcctccta
    19681 cttctttcca gttagtaatc tttcctgtat atataccttt aacttggtcc attgtcaggt
    19741 tttttactga gtttgcagta ttagttatca atgctatacc atctattgct attttagttt
    19801 cctttaatcc agcacttttt tcttcatctt ttaaatctct tgaactcatt cctatttcag
    19861 atgttccttc tatggcattt tttatacctc cggaagaacc aatctgttgt acttctacac
    19921 taacaccttg atttttttct tgaaactttt ctccgatagt ttccataaga ggacctattg
    19981 aagttgaacc tgaaacagaa actttgcttg aatctcctga atcacctgaa cctcctgagt
    20041 tagaactaca tcctactgct aaagctccaa ctaacattgt actagctaaa agaacgccta
    20101 ttttcttttt aaacatttta cttacccccg cttatatttt aattttttgt tgtcattttt
    20161 ctatatttta ataatacaga ttctttgtta aatgaatatt agagcagggt taagtaaatg
    20221 taaatattgt taaatacatg taaaaactta attttatatg atttattctt aattagatta
    20281 actttttaaa gttactttag gcagatataa acacaataca actcatatta tcagaagtct
    20341 tcattgaagc tatcaaccta tcacatccta taattctttt tctagtttat taactaattc
    20401 agcaaataca tttaaggctt cctctataga ttctttttta gtcatatcta caccagcaga
    20461 ttttaattgt tctagtgggt attctgaccc tccacttttt aagaagttta tatatctatc
    20521 tactgcagta cttccttctg aaagtatctg ttgacttaaa gcacttgcag cagaaaaccc
    20581 tgtggcatac ttatatacat agaagtttga ataaaaatga ggtattctcg cccattctat
    20641 tcctatttgt ttatctacta ttgcagattt tccgtaatac ttttcattta atttgtagta
    20701 tatattagta aattcatcag cagtaagtgg ttctccactc tctacccttt gatgtacaat
    20761 tttttcaaat tcagcaaaca tagtttgtct atgaactgta gttctaaatt gttctaaata
    20821 ataatttaat aagtatatag tttcatcctt agacttagaa ttttctaata aataatttat
    20881 aagaagtaat tcatttaatg tagatgcaac ttctgcaacg aatatcttat agtccgaata
    20941 taaaaatggt tgacttgttc ttgaataatg actgtgaaca gagtgcccta attcatgaat
    21001 taaagtgaat agagaattaa ggtcattttt atagctcatt aatatataag gatgtgaatc
    21061 ataacttccc caagaataag ctcctccttt tttaccttca ttatcatata catcaatcca
    21121 cccttcatca aaagcacttt ttatgacctt caagtatttt tctcctaaag gtttaagtgc
    21181 ctctaatatg atatcctgtg ctttatcata tggtatatcc atgtcaaatt tatctgtcaa
    21241 aggaacatac aaatcataca tgtgtatttc attcaatcct aaaaatttct ttttaagctc
    21301 aacatattta tttagattag gaatactttt atctatagct gatataagat tattgtaaac
    21361 atctaaactt acatcatctg aaaataaaga cgcttctatt gctgaattat actttctagt
    21421 ttttgcgtaa aatatctctg attttattcc accataaagc gttgaggcaa atgtattttt
    21481 atatttctca tatgttgaaa attcaccttc aaaagcgtct tttctaactc tactattctt
    21541 actttttata aataaagagt agtttgaatg agaaagctta accttattac cctcctcatc
    21601 ttctatctca ggaaattcca tatctgcata tgctaacata tcgtatacat tttctggaat
    21661 tgtcgataat tctgatgtag ctgcaagtat ttcttcctct cgttcactca aagtatgtgg
    21721 tttatctctc aatatatcct ttatatattt tgtataaaag gatagcttct catcttttaa
    21781 gtattcactt aacttattct cttctatcgc cagtatttct ggaactatat aagaagtagc
    21841 cacacctaaa tcagtagata acatttctgt cttagtggct cttccttgat tatcattaat
    21901 tctagtatct tcatgttgtt tcatatgagt atatacatat aaattttgaa caatcctaga
    21961 tgcattttca gagatattta atactttata taagttctcc ttactctcag aaagcttccc
    22021 tttatattct tttacttctt ggataagatt ttttacttta cttatgtctt tttcaatttc
    22081 ctctttactg gagtacatct tatctatctt ccatttgtac ttttcttcta ttgtacttct
    22141 atcagttccc atatcataca cctctatttt aatttgtttt atattttatt cactttacaa
    22201 aatatattgt atatatttta gtccaaataa tctacattta ttactccttc tatcatattt
    22261 atttctttca taacgtcgtg taagtgtatt ctcttagcat aagatacaaa aaaattaacc
    22321 ttccagtatt cttcattacg acattctatt tccatatttc ttacgataag ttgatttttt
    22381 tcaaatttat catataaaaa aggtatagag tccaaattgc ttaattttaa tatcaataca
    22441 ttcttttctt ggctaaataa aaacgtattg attatcctaa gcacaaataa tgctatcatt
    22501 acaaataatc cacctagaat tgccaattca taataaccat atccaactgc tataccaata
    22561 catgcagttg cccacatccc agcagctgta gtaagccctt ttactccacc tttatttttt
    22621 aaaattgctc ctgcacctaa aaaaccaatt ccagataata cttgagcagg cattcttgct
    22681 gggtcagcat ttgtatacaa attataatca aagaataatt gaatcgaagt tattgaaaca
    22741 atacaagaac ctattgcaat tagtatatgt gtcctaaatc ctgcaaactg tcttattttt
    22801 tctctctcta agcctattat tcctcctatt attagtgcta atagtaacct tattgctatc
    22861 tccttacttc ctatatcaat cataatataa aaccctcctg acttaattac aacttatgtc
    22921 ctacagataa tttacacatc agaaatacta ttttatatct taatattatt attaatatat
    22981 gtaaaatata gccatctgtt aatttttgat acaaaaaaac ccacaactgt gggctatttg
    23041 cttaggtaca tgtatttatt tattaatata tctattttct tacttgattc tactaactct
    23101 tcttgaaata tttctggata ttctatgtat gtatttatct cttccctaac tgcttcaatc
    23161 tcatttttta acgcttctaa ttcattgttc gccataataa tcaccactat tacaaatttg
    23221 tatattttta tatacatttt atcaaacaat attatatttt gtcaatatta tgtcaataaa
    23281 acttactata aaaatgcttt aatttaggta ttgtatacct ggatactaag cctacaaaca
    23341 taccagttac taatgatgcc attaacataa atggtaagta ccctatagca agtatatttt
    23401 ctatgattaa acttgcaact attaattgac ctatattatg ccctattgca cctataatgc
    23461 ttatccctat tagtgataaa tcttttattt tactcgctaa atacataaat aaaagactca
    23521 gataagctcc tcctatacta aatagtagat atgataaagg gcctgcaaat attgatgaca
    23581 aaattaccct gatagtaaca attataacag tatctttgac acctaacaac attaatgaaa
    23641 ttaaagttat aatattgctc aaacccaatt ttgcccctgg aaatataact ataagaggat
    23701 ttggtatata tgtctctagt acatataaag ctaaactata ccctatcata agacctaaaa
    23761 atgtcatttt tctagttttc attaagttaa ctcctccaaa ttttatctat catcttacat
    23821 tataatacaa aatatataaa aacacctaat ttagactaaa atatttttaa tctatttaga
    23881 ataaatatta gttatttcaa tgtgcaatta cctctataaa tcttgacttt ttgtatcata
    23941 cttgttacaa ttttaatgat aatgaatttc attaggaggt gttaataatg actgtttacg
    24001 atttaaaaat aggagaaaaa ggatttatta atagtataga tggagatatt aaattatcta
    24061 aaagattgct tgcacttgga tgtataaata gtactgaaat agaagttaaa aaggttgccc
    24121 cacttggaga cccaattata attagattta gaggctttga ccttgcgatt agaaaatctg
    24181 atgctaaaaa tatttcttta caataataga ggaggagcta cccgtgatta atgtagcatt
    24241 attaggaaac ccaaatgtcg gtaaaactac tgtttttaat ctacttacag gttcaaatca
    24301 atatgtaggt aactggcctg gtgttactat agaaaaaaaa gaaggatttc ttggaaaaga
    24361 gataaaggtt gtcgatttac ctggtatata tgcaatggat actttctcaa atgaagaaaa
    24421 ggtttctaaa tcatatttag aaaatgaaga tgttgatgta atagtaaatg tagttgatgc
    24481 ttctaattta tcaagaaacc tttacttaac aactcaactt atgcaattta acaagcctat
    24541 agttatattg cttaatatgt tagacattgc agaatctaaa ggagttaata tagatgccaa
    24601 aaaattaagt gaagaacttg gagtaattgt tgttccaatt atagcaaaaa agaaaaatgg
    24661 tgttgatagg atagaagaag agattaaggg agctacgcaa aaagctttta tatatcagaa
    24721 aaactttgga agtgaaacag aaacatataa aaaattagaa tccattttat cgagatgtac
    24781 aaaaactaca tatacacaaa agaaatctat cagtgataag atagacaata tagtcttgaa
    24841 tcctatatta gcatatccga tatttattgg tgtattgttc ctcttattta aatttacctt
    24901 tgactgggtc ggaggaccac tacaagaggg atttgctagt cttatagagg cttatatttc
    24961 tgcacctgtt agtgatatgt tatctaactc tagcccatgg tttaaatcac ttatagttga
    25021 tggaataatt ggaggtgttg gtggtacttt accattcttc cctttaatat ttacattatt
    25081 ctttggtata tcgttgtttg aagatagtgg atacatgtca agaacagcat ttttaatgga
    25141 taaagttatg agaaaggttg gtctatctgg taaagctttt atacctatag ttatgggtat
    25201 gggatgttct tctccagcta taatggcaac tagaacattg gaaagcgaaa aagatagaag
    25261 agttacagct cttattgcac cacttatgac ttgtggagca aagctaccta tatatgcact
    25321 atttgtagct atattctttc cacataatgc tgctttagta actacgtcac tgtatttggt
    25381 gggtatagtt atggcaattg tagttgcatt attcttaaat aaaacagcct ttaaaacaga
    25441 ggctgaacca tttatattag aattaccaga atatagaatc cctactatag atgcacttat
    25501 taaaaatact tggaataaat caaaaggttt cttgataaga gttgttactg tcatgtttgc
    25561 aatgtcagtc gtaatttggg gactttcttc attcaatgtt tttggatata cagaagatat
    25621 aaatgtaagc ttcttggcta ctctaggtca tattatatct cctatattca aacctcttgg
    25681 ttttgatgat tggagaactt cagttgctat tttaagtggt cttggagcaa aagaaatcgt
    25741 agtaaactct ttaaatatac tatatggtaa tctaactgtt gtcttaccaa caatatttaa
    25801 tggagttact gcatatacat tcttgatatt tactgcttta tatactccat gtatagctgc
    25861 acttgcaact atgaaaaaag aatatggcaa taaaatgatg tttacatcat ttgcatatca
    25921 atttatacta gcatggataa tggcttttat agttaaaagc ataggtggag tactatttat
    25981 gggtaattca ataatggaag ctttgatagg tggaataatt atagtccttg cactctttat
    26041 tctgtttaat aaattcaagt ctacaaaaaa cggaaatgtc tgtagtggat gttctatttg
    26101 ctctggatgt ccaagtgcta attcttgctc aagtaaggaa gacagcaata aaaaaagtaa
    26161 tacacactaa tatataaata ttttagttta aaataagata aaagtgggtg tctcaaaatt
    26221 aactttttag ttcatgaggc actctttttt atataaaatc aaatatattt tcatcatttc
    26281 aacagtcaaa aagaggctta tctctatttt gagacaacct ctttttttgt tacttattca
    26341 gtttcttgct acctaacttc atgtggaacc tagtatcttt tggaacactt gggtctacgc
    26401 ctcttaatgt aggtatttcc ccacatatta gttgtatagg tactataaaa ttaaatgttt
    26461 gatagtaagg attatctgtg atatcaatct ttaaattttt atcattttct gtaacatctc
    26521 tacctatagc aaatacattt tccgtccaac cagaaagtac atcaatcatc ttagttactc
    26581 taggttcttt tcctgtatct agtataaata tagtagaatc actatttata gcattatata
    26641 taccgtgtat aaactcttca aactcatacc cagttacagg tattctcatt gtttccaata
    26701 attttaatgc tgcttctaat gtatctccat atatatcaga gtgacctatt attcttattt
    26761 ctttagaatt aactaacttt tctttgtttc tttcaatcca ttgtttagag agcttataca
    26821 ctgcttcaaa tctattaatt gcatctaata tcttatttat ttcctcattg tacttttcac
    26881 tagatattgt acctttttct ctagctattt gaagacctaa taacattaag tttaactttg
    26941 tacaataata tccttttgtt ttagctccag atttttcttc accacaattt actgttaata
    27001 tataatcaga aatctcatct attaaagcat tcttacaacc tgccatagat gctattttac
    27061 aacccttatc ttcagctaac ttcatagcgt tgtatgtaga ataactactc ccaccttgag
    27121 atacacctac tactagggta ttttcattat caaacttgaa tgtatcttca gttatcataa
    27181 atggatacat tttcacaaca tctatatcta ataaattttg taaatatggt tgcacttgca
    27241 ctcctgagtg atagctagta cctgaacctg ttattataat cttttttaga tttgctctct
    27301 taacttcatt aaataagcta tctgcattag atataatttc tctcattcta actggtgttt
    27361 ctaacatata atcttgtatt gtcattttat acaccttcct tatctatata ttcaataaat
    27421 tataattaat ttctataaca aacctatagc atgtaaagcg attcctgtta ctatagtgcc
    27481 tatagttaat tttgttacac ttacctttct cttcaacatg taatatatta aaaatgcaaa
    27541 ggttaaaggt aacatattag gtattatttt atctagtata tcttgtaaaa ttacttctgc
    27601 accatttaag ttaaatttca aaggagtagt tatactcaac atagttgcaa ccattcctcc
    27661 tacaacgaat aaaccgacag ttgtagccat agacattatc ttctccataa gtccctctct
    27721 ttgtattctt tcaagagaat ttacccctac tttataacca tacttaagtc caaatattct
    27781 aagggcaaat gctggtatat tgtataataa taagaataat agtggtccaa agatattgcc
    27841 ttcttttgca agtgatactc ctactcctgc tgctataatc ctaaatgttc cccagaataa
    27901 tgaatcacca attcctgcaa gaggtcccat aagagctgtt ttaacagcac taattgaact
    27961 ttcatcaaat tctggattat tagcattttc ttcttccata gctgctgtta tacctaatat
    28021 agcaggaact accactgtcg ttgtattaaa aatctctaaa tgcctttcta ttgcttttgc
    28081 ttgattttct ttcttattat atagcttctt tattgcaggt aacattgaat aacaataacc
    28141 taagttttgc attctttcgt aattaaatgc accttgaaga gcaaatgaac gccaaaacat
    28201 acttcttaat tctttttttg ttaatttatt caaagtcaat ctctccttcc gttaaagtat
    28261 tcattgctaa ttgaggattt tgattatttt ctttgttatt gatataaaag tttagtacaa
    28321 atgctacaca tactccaaat aaagctatag cagttatatc taatccacca taagatgcta
    28381 acaaaaaccc tataaagaaa aatacagaca tcttttttga aatcaacata tccataagca
    28441 acgcaaaacc tactgctggc aacaatgttc ctgcaacacc caatccgtct aatataactt
    28501 taggtattgc atttatcaat gttgtaactt tatcagcacc aatcataatt gctaaaaagg
    28561 ttggaactga tgtacttaaa aagaataata ttactggcaa ccacatcatt atggtaactt
    28621 tcttaaaatc agcctgttta gcatagaaaa ctgccttttg agaaaagtac gaatttatta
    28681 ttctaactaa tactccaaga gattgtgcta atactgctac tggcactgcc actgctaaag
    28741 caacttctgc accattacct gataatatag caaaagctgt acccaataca cctcctgtta
    28801 ctacgtctgg aggagtagct gcacctatcc ctgcaattcc catccaaatt aattcaagct
    28861 gtgcaccaat cataattcct gattttatgt ctcctaaaac taatccaact aacatacctg
    28921 taactattgg cctatcaaac atagattgac caaatatacg cccatctagt ataccaactc
    28981 ctgctataat agctaacata gtagcttgta ctaacatatt gaacctccat tttaacttag
    29041 tttaatttac attaaattac attacatttt tgattttatt tcgattttct aataactcat
    29101 ttacataaac aattccattt tttgcttcct ctaagcctga tttaactatt agttttgtat
    29161 cctcctcaac taataagaat atagataaaa gcattggtaa atttacgcct gtcaacaatt
    29221 caaatttgaa cccttgcaaa agtaagtttg tacatatatt tgccacactt ccaccaaaca
    29281 catctgttaa aacaacaaat tcttcactat ctttattatt tgataatata cttctaattt
    29341 ctttctcagc atcatctgct gatttatctt ttgtcataca aaaatactca atattagcta
    29401 atgaaccaat tattaattta cttgtgtcaa caagttctct agacaattct ccatgtgttg
    29461 caattagaaa ttttttcatt ctctttctcc taaattattt tattaaatct gatagttgtt
    29521 tctttttgtc tctaggtaac attctaagtt ctatatcaat tcctaaatca cctatttctt
    29581 taaagttatt tatatcatca tcatcaacag caactaaatc tgtaagtttc ttttttcctt
    29641 cttcatatct taaccctcct acatttattt cttttaatac tcctcctgaa tttttagcta
    29701 caactaaagc atctctggta ttttttacta acactaatac attgcttttc tttgaagaag
    29761 caaatttttc tactatttct ccagattcct ctacccccct tatatataaa gttacattgc
    29821 taggtttcgc taaattcagt gacatagctt tcatttggtc attttttgct tcatcatttg
    29881 caactagtat tacatttata cctagtgatg ttgcccatcc atatgcaacc tgaccatgta
    29941 tcaatctgtc atcaattctc atcaaagata ccattttccc caaatcctcc atcatacatt
    30001 aaaattttta ttgtttatac tctcatttat ctataaaagc aaaaagtatg ccaaatactg
    30061 gcatactatt ttaaatttca tttacttatt ttgtaatctt gtattttact gtggtagacc
    30121 atagataata ttataaatat atcctatctc tgaaattggt atttgcacac tataactctt
    30181 ttctagcaca ctaaatgcat tttttattag ttttacttca ctatgatggt ttttgattaa
    30241 caaatccata tcagtatact cttcaatttc agtttgtcta actaatcttt caatcataca
    30301 acttaaatga acatataaag aaatcttttt atcatttgga agttttttct tttgaatatt
    30361 ttctatagca ttgattcctt cttctattct ctctattatc ttttttgaat ctaaaatagt
    30421 tagactgtct attactctaa ttaatgaaaa attctttaca atagaatcat ttatgttctt
    30481 tattttttca ttttctacta ctctactaaa tattctaaaa acatcatctt ctccttttcc
    30541 tgatattata tcctctaaca atataaaatc caccccattc acatttggat tagcagttcc
    30601 tactatagcc atcacatcat acatttcaaa aactggttct ttatctccca tagaaacaag
    30661 actatcataa tcataaggta ttattttgac atcaactatc ccaattaaac tttcggaaag
    30721 tagatgttgg atttttatag cagtacctat ccctgtaaaa caagatgata ttatagcata
    30781 ttctttattt ttttgaggat aatacaattg tatattaggt ttagtttctt catccataat
    30841 ttctgcaatc tgttcaatgt ttttaccttg cattaataaa tttcctactt ctagtgcaga
    30901 aatagtggat atttggtcta taaataacat tggcccattt atcttatttt ttaaatcttc
    30961 atagataaga ttaagagagc ccatatcaaa taacacaatt aaaccattct ttatcctatc
    31021 tccttctagg tatctagaca tttgttttga tacatcagca atggtagcat ctataggcat
    31081 atcaatagct gtaaatatat ttttacccaa catccgatta catacattcg ccatactact
    31141 ggcagtagca tatccatgtg ctataatagt cgcattaact tgattaaatt tcattcttat
    31201 aattgttgat ttaataaata taatcattat gattgtatct acattattaa gatacatatc
    31261 caatctattc gttattaatg ttgatatttt acttattata gagtattctt tgtaactctc
    31321 tttttttaaa tatattagta atttactttc taaagcttta tactcatcag ataattgata
    31381 agattcgtcc ttcaaataaa gataagacga aaatgccaat acattattcc catcgtattt
    31441 tatattgtta ttaacttcta aatttctaaa tatatcctgc attatcgata ttataagctc
    31501 cataagtacg ttattatttt catcattatt tgaaaagact aaattgtcca ttagtctaat
    31561 cattagatta gaactctttt tactaaatat ttcttttgtt atttcatttt tttctagtct
    31621 atcaaagtag tctaaacatt ttatgaaact ttgatagatt atatttcttt tatttcttcc
    31681 atttgacatt tcaatactaa attgtttagg agaaaagata atgcttttgc ttttaccact
    31741 ttggaattga aatatatcta cattttctat ataaagattt ctagggaggt cttgaagagt
    31801 aacaaaaata tttccatttc ttctttgatt ttttgcataa gctcgtccac atgcatattt
    31861 tattacattt ttacattcac ctatattccc cttgaatttt atctttaaaa tcgtatttaa
    31921 tgtaatttga gttatctcta taggcaagcc aagtacctta ctttcttcat aaataaaatg
    31981 ttctacaatc tttctttttt caatagtccc tctttcttct atattgggaa cataaatatt
    32041 tatgggtatt cttcttaaaa aagtttgcaa aaatttggat agactttctg ttgttgcaaa
    32101 tattaatctt accctagctt ttctaattac tccattctct ccaatcctag aaaacgtacc
    32161 tgtatccata aatgtaaata acttttcctg accttcacta tttaatctat gtacttcatc
    32221 taaaaataaa acaccaccat ctgattgttc tattagacct gctctagatt catttgctcc
    32281 agtatatgct ccttttacat gcccaaataa tagactagac aataactcca aattattata
    32341 atactgcgca cagtttaaca cattgtatgg agaatttttt tctaatacat tactgcttac
    32401 actatatttg tatattatct tggctaaaaa agtttttcct actccacttt cacctgtcag
    32461 cattattgta agtccgccat ttggataaag tacagcggtt tttatttgtt cgattacttc
    32521 ttttaagcta ttatcatatc ctatgatact attaaacaca gtctcttgct ttaataattt
    32581 acactcattt ttaagttcat ctaaattttc ataaatactt ttttttgtag gtcctactac
    32641 atcatttatt ttatttttag gtaaaaaaat aacaggtcta ctgtttatct ttataagatt
    32701 atcagatttt actaaatcat ttaataatct acttgattga gttctactta aattaaactt
    32761 ttttactata tcttctgtta ttatgccact ttctttttta agttcatcaa agttgttaaa
    32821 taatacacat tcatttacaa aacttaaaat ttcatccctc atgttcaacc tccaatacaa
    32881 atatcaataa tactataata ttagtatatc atttatatat ttatgaaaaa gataattata
    32941 ttcctatctt gattctcttt tgtaaggttg taaaatcaaa ctttttataa gtataaaatc
    33001 tagatttttt aacataatct caaggttctt atctttccat atatctgaca tagaatttat
    33061 ataagaatat atttattgta tttattataa taaaaagtaa aacattatag gtattttaaa
    33121 acttattagc gaagaaaata tttgtaactt ttattacctt atgaatatta tttcaaattt
    33181 aaaaaggcta taaataaatt tatttttaaa taaatctact tataacctca caataatact
    33241 tataaatttt attttttcac aatgatatat ctcaaatata gttaaaaata tatctattta
    33301 attattaata tttaagtaat aaattctcac tcaatcatta aattcctttt tatagtaaat
    33361 tactcttatt ttacttatac caaatacatt tatgtataga tataaataag tactaaaaaa
    33421 acaattcaag aaaccatata gtaaacttaa ttacatctgt tatttgctca tatctttaga
    33481 cttatctata caagcaacaa tagattttat aactgctgct ctaaagcctt cttcttctaa
    33541 taccttaaca gcttctatag tagttcctgc tggagaacaa accatatctt ttaattcacc
    33601 tggatgtttt ccagtttcaa gaaccatctt agctgacccc attactgctt gagatgcaaa
    33661 tttataagct tgttgtcttg gcattccagc taatactgct gcatctgcca tagcttctat
    33721 aaacataaat acatatgcag gagaagaacc actaatacct gtaactgtat ccattaaata
    33781 ttctgatact atttctgcct caccaaaaga ttcaaacaga gccttaatca tatttaactc
    33841 ttcatctgtt acattactat ttttacaaag agctgacatt ccctcgccaa caagggctgg
    33901 agtattaggc attactctta ctattttttt atctgaacct ataaaacttt ccagactttc
    33961 tatagtttta cctgctgcta tagatactat tattttattt gcatctatta attctttaag
    34021 tggagttaaa gccttttcgc atatattagg tttaagtgct actattacca catcagaatt
    34081 tgtaacagct acctttgaat cagttgtagt atttattcca aattcttctt ttactttatc
    34141 taaggcaact tggcttaaat ctgatacagt tatatcttct gcctttacta atcctgactg
    34201 tactatgcca cctatcattg cacttcccat attccctgaa ccaataaatc ctaaagtttt
    34261 catatcaaat ctcctttttt ctattagttt tattttaaag tagctaaatt gactttattt
    34321 taactgctta aaattatctt atttttataa aatataacct tattataact ataataaatt
    34381 attcttttgt atgtattact tactaatatg tgtatgattt actaaatata aatttaacga
    34441 ttttttcatt aaacttaacg agctaacttc ctatttagaa agttagctca taattaatct
    34501 aaaattttaa aaatggaatc aaaccattta tactttatta tatcagttac gattttatat
    34561 agtaagaagt tttatttaca agcaaaccta gaatgtttgc tcagttcttc ctattatctc
    34621 atcttgtagt gttctactta aattattgaa atggtcacta tatcctgcaa ctctaactat
    34681 taagtcttta taatccttag gattcttttg agcctctaac agtacatttt tatcaaatac
    34741 attaaattgt atatgatgac catccatatt aaagtatgct cttactagat tagccatatt
    34801 atctaaccct ttttctcctt gaactactga tggtgcaaat ctttgattta ataatgttcc
    34861 accagttttt atgtggtcca tcttagcaca agattttata actgctgtag gtccattagt
    34921 atctccacct ttttcaggtg aaattccctc tgaaactggt tttgcacaaa gtctaccatt
    34981 tggacttgct cccataattt ctccaaagta tatatgacaa gttgttggta acatatctac
    35041 tctgtattct ccaccacaaa cagttagtct accagttacc tcattgtaat atgcattgaa
    35101 tatctcttgc attatttcat cagcataatc atcatcattt ccatatttag gagtcttatt
    35161 tgaaactaaa ttaagtatag cttcatatcc ttcaaaatta gcatcaagtg ctttaagcaa
    35221 tgtatccata tcgaattttt tcttatcaaa tacattatat tttattgctg ttaaactatc
    35281 tgttatagtt cctataccaa caccttgtat atatctagtg ttgtatcttg ctccacctct
    35341 ttggtaatcc ttgccttttt ctatacaatc atcaactatt gtagacataa gtggacaagg
    35401 catgtgtttt gcacatatat tttctatcac agcatttcct tctattttta tgtcaatcat
    35461 ataatgtaat tgctttttaa atgcttcaaa tagttcctca taagattgga aatttctagg
    35521 gtctccagtt tcaataccta tttgtttttt actaattggg tcatatccat tatttaatgt
    35581 taattcaagt atttttggaa tgttcatata tccagttaga acatatgctt ctttaccaaa
    35641 acatccagtt tcaacacaac cactggttcc accaagtctt gcatcttcta tagtttttcc
    35701 agcttccata agctcttgaa ctattgcttc agtattataa aatgctggtt gaccccatcc
    35761 ttttcttgat atttcacaag ctctctttaa gaatttttga ggagtctttt tacttatttg
    35821 aacatttgaa ctaggttgta tcaatttcat ttcatccata acatcaagta ttatataact
    35881 aacttcatta acaccatctt gaccatctgg atttattcca ccagtgttta tatttgcaaa
    35941 atctgtatat gtgctacttt cttttaatgt tataccaact tttggtggtg ctggctgatt
    36001 gttaaattta acccataaac attcaagtaa ttcttgtgct ctatctctat ctagtattcc
    36061 attttctaca tctctttcat agaaaggatt tagatgttgg tcaagtcttc ctggagtaaa
    36121 agcatcccat atattaagtt cagtagtaac ccctatatga acaaaccaat acatttgtat
    36181 agcttgatga taagtttctg gtttgtgagc tggtacaaca tcacaagttt cagcaattag
    36241 taataaatct tttttcttag cttcatctgt ttcttcttct gccatttgtc ttgctttctc
    36301 agcatatctt ttacctaata taaccattgc atcacaagct attgccatag cttctaaatc
    36361 tgctttttta ttatatgcat ctaaatcatt aagaaaatct agctctttta atcttgcttc
    36421 tatgtctttc tttaaatcta aaaatccttt tttatatatt gtatctccac aaactgtatg
    36481 tcctggggct ctttgttcca taaactctgt aaacatacct gcttcatatg ctgctaacca
    36541 ctcaggtgtc attgcattta ttatcttatc tcttgtttgt ctttttttcc aaaaaggaat
    36601 tatttcttct ttatgtattt ttctagcttc ttctgatacg ctaaaattta ttatatctct
    36661 attgtgcatt acttctaaat cttccattgt gtgacaacaa atttctggat atgttggtgc
    36721 tccattagga gaatcacctt tttcacctac tattaattct ccatcattta tgctaagagt
    36781 tctattctca atataatgct taaatgataa agcacgaagt acaggaatct ctacacttcc
    36841 ctcatatttt ttataagctt ctgtcataag tactgctctt tcaatagata tgtgtggttc
    36901 tgcattaata ctttcttctc ttaatttttt agttctctca aaagttcctc ttgccatttt
    36961 attaccctcc aatcttagta ttgaaattac ttgcttcaaa cagtcttttt atttcctcta
    37021 atttttcttc tgatggtctt tgtagttctt ctccttcata ctttttataa attttatcat
    37081 acttgtgttt accaatatta tgatatggaa gaagacttac tgcttgtata ttcaaaggct
    37141 ttaaaaactc tatcattttt ttaacctcta aattttcatc atctacattt actcctacaa
    37201 ttaatggtat tcttatattt atgttaaccc ctaattcact taaaatctta acatttttca
    37261 gtattaagtc atttgatttt cctgtaaatt ttatgtgctt atcctcatca ataagcttaa
    37321 tatcatataa aaacaaatct gcacatttag caactctttc ataattttct gactttgcat
    37381 agccacatgt atcaattgct acatggattc cctttgattt gcacatgttt attactccac
    37441 aaatgaagtc catatcttga gccattactt ctccacctga tagtgttact ccaccacccg
    37501 attcttcata gaaaattcta tccttctcta tctctttaac caagtctctt acagaatact
    37561 cttgaccaac aatctctctt gcattgttta cacaatagtc taagcaagtt tcacaaaact
    37621 cacacttatc ttggtctaag catatttttg tttcaccttt gtaaattgcc ttatgagggc
    37681 atatatttat acatgcttca cattttgaac atctttcttc attatacaaa acttgttttg
    37741 tatatgtttg actctctgga ttatgacacc aaacacattc aagtggacac cctttaaaaa
    37801 atactgtact tcttatccct ggtccatcat gtatgctaca tttttgtaag tttataacta
    37861 atggattcat gatttttcac ccctaaataa tatccattcc attttttcat ttttatgcaa
    37921 ttcatcttca tagaatatac ctatgaaaag catttctaca attacataat actatatttg
    37981 ttattatttt gtctatatat ttttagtaat attaaaaaat tttattttat tatgatattt
    38041 atattgtttt tgtaaattaa atttaaatat ttattaaatt tatttttata agaaa
//
LOCUS       DAMKPG010000030        37645 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMKPG010000030 DAMKPG010000000
VERSION     DAMKPG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.032"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(122..382)
                     /locus_tag="PC621_001624"
     CDS             complement(122..382)
                     /locus_tag="PC621_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108785.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(645..998)
                     /locus_tag="PC621_001625"
     CDS             complement(645..998)
                     /locus_tag="PC621_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108786.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1015..6192)
                     /locus_tag="PC621_001626"
     CDS             complement(1015..6192)
                     /locus_tag="PC621_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HDF4108787.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(6214..7200)
                     /locus_tag="PC621_001627"
     CDS             complement(6214..7200)
                     /locus_tag="PC621_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF4108788.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(7201..7899)
                     /locus_tag="PC621_001628"
     CDS             complement(7201..7899)
                     /locus_tag="PC621_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF4108789.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7896..8948)
                     /locus_tag="PC621_001629"
     CDS             complement(7896..8948)
                     /locus_tag="PC621_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF4108790.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(8941..9369)
                     /locus_tag="PC621_001630"
     CDS             complement(8941..9369)
                     /locus_tag="PC621_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF4108791.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(9369..9695)
                     /locus_tag="PC621_001631"
     CDS             complement(9369..9695)
                     /locus_tag="PC621_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF4108792.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9711..11240)
                     /locus_tag="PC621_001632"
     CDS             complement(9711..11240)
                     /locus_tag="PC621_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF4108793.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11522..11944)
                     /locus_tag="PC621_001633"
     CDS             complement(11522..11944)
                     /locus_tag="PC621_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108794.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(11944..14397)
                     /locus_tag="PC621_001634"
     CDS             complement(11944..14397)
                     /locus_tag="PC621_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108795.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(14397..14564)
                     /locus_tag="PC621_001635"
     CDS             complement(14397..14564)
                     /locus_tag="PC621_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108796.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14591..15037)
                     /locus_tag="PC621_001636"
     CDS             complement(14591..15037)
                     /locus_tag="PC621_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF4108797.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15151..15579)
                     /locus_tag="PC621_001637"
     CDS             complement(15151..15579)
                     /locus_tag="PC621_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF4108798.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15593..16657)
                     /locus_tag="PC621_001638"
     CDS             complement(15593..16657)
                     /locus_tag="PC621_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF4108799.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16662..17105)
                     /locus_tag="PC621_001639"
     CDS             complement(16662..17105)
                     /locus_tag="PC621_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108800.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(17354..17794)
                     /locus_tag="PC621_001640"
     CDS             complement(17354..17794)
                     /locus_tag="PC621_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108801.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17899..18096)
                     /locus_tag="PC621_001641"
     CDS             complement(17899..18096)
                     /locus_tag="PC621_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4108802.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            18265..18585
                     /locus_tag="PC621_001642"
     CDS             18265..18585
                     /locus_tag="PC621_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF4108803.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18667..19173
                     /locus_tag="PC621_001643"
     CDS             18667..19173
                     /locus_tag="PC621_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108804.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19874..20629
                     /locus_tag="PC621_001644"
     CDS             19874..20629
                     /locus_tag="PC621_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF4108805.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20772..21200)
                     /locus_tag="PC621_001645"
     CDS             complement(20772..21200)
                     /locus_tag="PC621_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF4108806.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21621..21821)
                     /locus_tag="PC621_001646"
     CDS             complement(21621..21821)
                     /locus_tag="PC621_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF4108807.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22004..23173)
                     /locus_tag="PC621_001647"
     CDS             complement(22004..23173)
                     /locus_tag="PC621_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF4108808.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23409..24230
                     /locus_tag="PC621_001648"
     CDS             23409..24230
                     /locus_tag="PC621_001648"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF4108809.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(24306..25694)
                     /locus_tag="PC621_001649"
     CDS             complement(24306..25694)
                     /locus_tag="PC621_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF4108810.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25838..26596)
                     /locus_tag="PC621_001650"
     CDS             complement(25838..26596)
                     /locus_tag="PC621_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF4108811.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26598..27314)
                     /locus_tag="PC621_001651"
     CDS             complement(26598..27314)
                     /locus_tag="PC621_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF4108812.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            complement(27316..28023)
                     /locus_tag="PC621_001652"
     CDS             complement(27316..28023)
                     /locus_tag="PC621_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF4108813.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            complement(28140..28676)
                     /locus_tag="PC621_001653"
     CDS             complement(28140..28676)
                     /locus_tag="PC621_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF4108814.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28778..29848)
                     /locus_tag="PC621_001654"
     CDS             complement(28778..29848)
                     /locus_tag="PC621_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF4108815.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(29841..30899)
                     /locus_tag="PC621_001655"
     CDS             complement(29841..30899)
                     /locus_tag="PC621_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF4108816.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(30886..31218)
                     /locus_tag="PC621_001656"
     CDS             complement(30886..31218)
                     /locus_tag="PC621_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF4108817.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            31417..32538
                     /locus_tag="PC621_001657"
     CDS             31417..32538
                     /locus_tag="PC621_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF4108818.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(32666..34564)
                     /locus_tag="PC621_001658"
     CDS             complement(32666..34564)
                     /locus_tag="PC621_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF4108819.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            34879..35655
                     /locus_tag="PC621_001659"
     CDS             34879..35655
                     /locus_tag="PC621_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF4108820.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(35747..36499)
                     /gene="xth"
                     /locus_tag="PC621_001660"
     CDS             complement(35747..36499)
                     /gene="xth"
                     /locus_tag="PC621_001660"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF4108821.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(36660..37610)
                     /gene="galT"
                     /locus_tag="PC621_001661"
     CDS             complement(36660..37610)
                     /gene="galT"
                     /locus_tag="PC621_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF4108822.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
BASE COUNT        12067 a         6507 c         4001 g        15070 t
ORIGIN      
        1 ttactttttt taattaatat tttaaaatat aaaatccttc tcaataaaat agggcactat
       61 ctacaatact actttatctt ttcctaataa tatataatct ggtgctactg ctactttcat
      121 gttatacacc gttttgtaac atacctttta tatcagtaac atcttcctta atttcctcta
      181 catctgtttt cattattccc atttgaacaa gtatattttt gtttatttct tgttgttgtg
      241 tagataattc tataaaattt tctacagttt ttttatacat gtctctatct tcttttttct
      301 cctgcatagt atttttaaat aatagagcac atagtatacc tattgccccc agactactca
      361 attcggttat taatttatcc attatagcct cctattgtct aaatttttta tacttatagc
      421 tttaattttt gttatattct tacatcatgc aatattatat tttcaggatt atcaatttaa
      481 tcttctgtac tttaaattgt aggtttaatt tatagattga ataaataata aatatatata
      541 caatttctaa tataacaaaa aataagtaca aggcttactt attaaagtta agaccttgta
      601 ctttacataa aaaaaataac taatacatag ttattctaaa tttactattc aactggtatt
      661 tctaattctt tacatctaat tctaacttgc tctctaatta ctgctggtac ttcttctatt
      721 gtttttctgc ctcttaaaat taaagctaca tatacttcta ccattccact ctcacctcca
      781 gattttctta cactaaaagt gcttatatta cttgcttcat tgacagaact ttgacttaac
      841 aacatctcat atatttctgc atttgctata aaattatttg ttatatcatc atcttgcttt
      901 gcttgaccta tacttatgtc tgaaatgtct ttatactgca tttctacttt tctaccttgt
      961 ttgatttgtt ctattttatt tttatcgaat ttaaacaatt tttacacctc cattttaagc
     1021 ttgtggctcg ttgttttcag catcatatct aaaaatcata gatggacgtt ttatagctgt
     1081 ccatttatct cctttttcta ctatgatttt aggctgagca ctttttatgt tggcttcttc
     1141 taattcatag atttcttgat aacttgagtc tgattttttg atttcaacta gtttcatgtc
     1201 tttgaataag tcagatttat ttatttttac ttgaacttta gttttattta ttaattgata
     1261 tatagggtct ccaagattta aatcttcttc aagtcctcta tctagtgtaa ttgtaatatc
     1321 ttgttcatta gatgttgtat taactacctt gtacattcta ttacctatca taacataaga
     1381 atctttaatt atagaatttc cagtactctc tttaaaaata aataaagatg catataaatc
     1441 atcaccttta caataagctc taaatagtat cttattctct cctattttaa ttttatctgg
     1501 tggtacttca aaggatattt tttcagtagt taaatcactt acagatttta cactttcacc
     1561 atttataagc atttctattc tacttatatc tttattaggg tcttgtattt ttatcttaat
     1621 tgatgaaatt gatttttcag taatttctat agcagcattt gtcgttccta cttctgatat
     1681 aatatataat gtattgtctg ttgcaagtac atcctttact gtatttacat cttcagaaat
     1741 aggtactata gtaaattctt ttaaaaattc tactggttgt tcaattttat atacatccct
     1801 attttttcca gtaacccata ctgaattatc atttttaagt actaatatat ggtcacttcc
     1861 ataagatttt atttctctta tgtctgttag tgcttttgta aagaacttaa ttatagtatt
     1921 ttcactatcg cttcctaatc ctaaagctcc cttatcatta tatccttgta caaataattc
     1981 attacctgta gttactacta atgtatagtt tttaccacaa tgtacttctt tacagctaga
     2041 tatagttgta attctttgta attcagtaac tggattattg tgacctatac ctaattgata
     2101 ttcggaattt ttaccaatag accaaagact atcatcagat tttataaaat aagtaaagtt
     2161 atctccacat gaaacatgct ttacatctga tacattaatc tgcacaaatt catccacttg
     2221 atttatagac aagtcttgca ataattctcc atggtagttt gtaccacatc catacattgt
     2281 tccatcatta agtaatataa taatatgagt cttagaaata gatacttctt ttatatcttc
     2341 atatggaagc tcagtataaa ggtctccttt gtataaattt cctaatacta aaccataatc
     2401 tatgtctttt gtggtaggta tcactatctt attgtttaga agtataaagt tatgtctgtt
     2461 tttaatagat gtcaaatcac ccattacaaa attcatatgc ttataattaa atattctatt
     2521 atcattattt ctactatcgt atctatttcc aacacctaat tgtccatagt tatttaatcc
     2581 acaaatatat atatcattag tatcttttat tgctatattt gcatattcta aaggaagtat
     2641 tttctttact cttgtattgg tttttataaa tgtatctctt tttgttacat caccaacacc
     2701 taattgtcca ttattattaa aacctgtacc atataggtct ccattatcac ttacataatg
     2761 tccacatcta taattcattc caatatcctt tgcctttgat gctatttttt taatcttctc
     2821 tggagtctca cttcctgtac ctgaaccatt actacatttg cctgcagtat atacatcccc
     2881 ttcacttgta agtaaaagta ctgcattatt tctgtttgga gctactttta ctacatttga
     2941 aattggaatt tcagaaaatg aactttgatt gctattacta tttagaccta agcaataatc
     3001 agtatttaaa cctgtactat atgcagtatt atagattgtt attatatagg tatttatttc
     3061 atttggtata acttctttta cattttccat acactttata aaactatact ggtcagtagt
     3121 atttcccata cctagttgtg aattattatt gtatccacaa caatatgcaa tgtttgtgtc
     3181 ctttacaata aatgttgttg tatctgagca gtacacatct cttacattag ttatattgtt
     3241 tacagtaaat gtcaatacgt ctggatggtt gctttcgctt cctaagccca gttgcccatg
     3301 agtgttttct ccacatgaat ataatgtgtt atcatctttg attgcatgcg agtgagtagt
     3361 ccctaaaaca acatctctta cattttggat gtttactttg gtaaatgtat ttctatttac
     3421 tatatcacct aatcccagtt gtcctttggt atttaatcct gtggagtata gtgttccatc
     3481 atttctaatt gcaaatgttg tgttaccatc tatacttgtt gatattttct ttatattatc
     3541 aaggtttatc tgagtgaact tatttctatt tattgtgtca cctaatccta attgtccaga
     3601 actattattt cctgtggcat aggctgttcc atcatttttt actataaaca ttgtatttct
     3661 tgatgttgta atttctttta tatcatccat aaatataaat gagtttgatt gttcagttat
     3721 atttgtgaat ttttttactg gttcgttgtt atattcccct atcccaagtt tgttattctg
     3781 attcattcct gttacatata tattgttatt tttatctact actactacag tatttccttt
     3841 tacaattata tcttttatgt cttttaatcc tgtatcatag ttacttgtgc catctatatt
     3901 tgttgaattc ccaaattgcc cactattagc aatccctttt ccatataact tggaattact
     3961 atgatatatt ataaaattac ttcctaaatt agaactctca gaattaatac tagtataact
     4021 tctatgtcca gtaatggttg agccaaatgt ttttggatat ccagtaatat atgaacttcc
     4081 tttcttaaaa aataatatat tatttgacat ccctattact ttttcaattc cactaaaatc
     4141 agtctcttgt ataaaataag tattatgatt tgaatttgta gcctcaataa aagttccata
     4201 tcttgaataa tcagatatac ttggactgct atttaatgtt cttaaatatc catgatatcc
     4261 tgttaaataa acataacctc cttcgtcaat tattgcacaa ccatcataaa aaggaaaaac
     4321 attagttatt ttaggtaacg ttctaagtcc atttgatgta gaagatgttg aatttgttct
     4381 acattgagat actaatgctt tgctttggtt actattattt cctttaccta attgataatt
     4441 ataattgtat ccagaaacat atagtttccc ttcactgttt atcatatata cagaataatc
     4501 atatgctgct atcttttcaa tattacttat tccctgctta tatggataat tagaagtttt
     4561 ttctatatta ttataaggat tatttcccca tccatatact tcttttgagt gatttaaagc
     4621 tatgacataa ttgcttccta cacaaaaatc ctttacattc tctatttcta tttttgtaaa
     4681 tatatttcta ttaacttcat ctcttaaacc caattgtcca ttactattta aaccacatga
     4741 atataatgtt ccattattct taagcacaaa agttgagttt ccgtttcctt ttatatccaa
     4801 aacattatct atatccacct tagtcataac tgacctgtta acagtatctc ccaatcctaa
     4861 ctgtccataa ttatttaaac cacaagcata caatgtacca tcattcataa caataaacat
     4921 attgctatct gttaactcta tcttcttata atcttgaaaa ttatcactta actttacatt
     4981 taaaaattca ctattgtcct ttgtaacata taaataatca tcattaacca ataaacctga
     5041 ataattatct aaatttgaag attgcacatt tgaaaactca tacagaaaag ttgtatgaaa
     5101 aaatgaacca agttgacctg ctaaattaca gccagataca aatatctctt ttgaaccatt
     5161 tattaaaaaa ttaaattgag aaccacattt aatatctttt atattttcta cattaacctt
     5221 agcaaaagta tttcttgatg ctacatcttt attggcattg gctagttgac cataattgtt
     5281 ttgtcctgta ctaaacagtg tattatcgta ttttaatatc atagtatgat tatttccaca
     5341 agaagcatac ttaacattat ttatactaga aagagtaaat acaattgaat tattattatt
     5401 tcctgtacca agctgaccaa ctccattata gcctgtagta tacatagttc catcattctt
     5461 aataagaatt gtatgactac taccacacgc tatctgtttt acattatcca taccttctat
     5521 tttattaaat acacttttat ttaaagtact attaattccc aactgcccat ttgaatttaa
     5581 tccacatgca tgtgctgtcc catccatttt tatgataaat acagattgat tataagtcac
     5641 tacatctttc acactatcta tatttacttt agtaaaagta gctctattgt tagtatcacc
     5701 tagtccaagt tgaccacttg agtttaaacc tgctgaatat aatgttccat catttttaag
     5761 cacaaatgta aagtcataac cacatgcaac agtttttaca tcatctattg gtagcttagt
     5821 aaattcataa tatactgtag actcaattac acctaattga cctacattac ttgttcctgc
     5881 tccatatgca aatccatctt tagttactgc tacactgtgt tttgaaccac aagatacata
     5941 ttttacatta tcaattttta ctttactaaa catccttctc tctgacgaat cttttccaag
     6001 tcctagttga aaacctgtat tatcgccaca agcatataaa ttaccatctg attttaaacc
     6061 aaaggtacta ttacttatac cagtaaaaaa ttctacaaaa tcataatcat tatatctttt
     6121 atcaaaatca tcaatcaata tgttcttatc attaaaataa gcaccacgct gtaaaagttt
     6181 attttgcttc attattagta ctacctcctt tatttatctt atttcatatg aatatttact
     6241 tagagttata tctttttgtt cttttaaata agtgttacta gattttgtct caacaaagtt
     6301 attatccata tcatgtatat atctaactct atttgcacta aatggtaata ctacatctct
     6361 aaaaatcaca ttaccaattt cacctgttat acattttcta tttaaatcta acttaagccc
     6421 ttctatagct actatgttat ttccatttgc taatgtatca taccaataac cagcctcatc
     6481 tacagttact tctgactcta attctaggta tattaatata tctagcactt tttcagataa
     6541 tttgttatag ctatttttta atactgatat atcagtttgt atttgaccac ttataacatc
     6601 atcaaaagat atattttcta tttttttaac tacctcattt atagctcctg ataaatcttt
     6661 agaatctgta gttaatatat ctgtatctcc aatttttact tttatttctt ttatattttt
     6721 atcaaactta tcatctaaat cttgaatatc cttttgagtt acaaaaacca acgtcgggtc
     6781 tacttctaat gtaacatttg aagtattgga caattgtaaa atcattttta caaccaaatc
     6841 tttaatactg ccatcttcag cacgtggctt atacgtctct ggatacttac ctatagctat
     6901 tatattattc tcattatcca gaacagcagc ttctcttatc ataaatccac caactgttcc
     6961 tggtaaaata gtctctataa ctatccaatt aggatttttt tcatcaattc ttagagaatt
     7021 tatatttcct tcccaaacta cattctttaa agcagtttgt tcctctgttg gctcataata
     7081 ttctccatta ctatctccaa cttgaaactt ggttaatcca acaagctctc ctagtgcagt
     7141 tgcatttgct attttttctc taccaatttt ggttaatatt atataaaatt tttcttccaa
     7201 ttataccacc tccgatttag gataaactac tacattttct acgcctgtat catttgcaat
     7261 agctatatta atcttacaat tattttcaat gcatttaatg cttaaaggat aaacctttat
     7321 ttcttctcta cttaacattc cagctccaca gtttacaaca gcactacatt ttataattgg
     7381 ttcaaagtat ggatgaactt ttacctcttc actacatacc atacctccac cacaatagac
     7441 catactttta tctagtatta ttggttctaa atagtgtaat aagtgagcag gttttactct
     7501 ttcaatcatc tcgctacaat ctaaaagagt tttatagtca cagttatttg ctataaaact
     7561 caatacgaat gtaaattcat cactataaac ttttatatct gtttctgatt ttgtatatgc
     7621 ctcacatata ctttttataa cttcaatagt actagtacct ctgcttctca ttttagcttt
     7681 tatattgcta cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga gtatgtcttc
     7741 ccacatatct aatccccatg tcgctgtatc aacaaataat tggttaaagg tatcatcaat
     7801 atcttcttta agaatattta attctgtatc gtatgcacct tgtatctcct ctacaatgta
     7861 atttctatca aatgatggta gtttatcaat tagcttcatt gattttccac ctcactaaaa
     7921 ttaacagttg ttacactagg tattttatct tcattaatag ttatattatc tgtacttcca
     7981 tttataagta gattacttaa atcgtgtaca ccagtagtat ttataagtat tcccatgact
     8041 tttgtataga ttatctctcc tctaatatct ctaaagtatg tatttatact ttctaggaaa
     8101 gattctttta cattgtctaa tgtatatcca tcttctagtt tcattactgc acttatactt
     8161 atttctattg gtaatggtgt cacaactgtt atagttggtc caataggctt ctcttcatct
     8221 atatgttgct gacacctttc aatcgtttct gtatcaacag cttgattatt ttccccaaat
     8281 atcaagacct taactgttcc tggaccatcc catcttggat aaaccttaac attatagact
     8341 ccatctactt ccaaagccca ttcttcatag tgagctttat ttccacttgt agcttgattt
     8401 ctttggattt tataaaatct ttcttttagt tcttcatctg tctctatatc tgtaccacct
     8461 tcaaaacttc ttgtgttagt aatctttgtt actccagata tattatcaac tagtttaaat
     8521 tcacaatttg taggtaaatt atatttctta ccaacttcca gagcttgaac tgggctattg
     8581 tcaccttctt cactaccaat agttacatct tttattacta caaatagtaa atctctatat
     8641 gatattattg tgccattatt tattacagtt cctttctctc caataaattc aacttctcca
     8701 tttgactctg tacctaactt tctatataca ccaaattcat tgactctttt atcaagaaac
     8761 tggttatatg tgtcttgtat aaaagccatt ttatgtatct ttgaaagttc tagatatatc
     8821 ttcgaaagtt ctagattatt tccagatacc atgttgttta gaaaagaacc ttctccttta
     8881 taaatatcaa gattaatatt ttcaagagtt ctatttttta ttacttcata tgtctggtca
     8941 ctatacattt atttccacct ccccatagat agtggatact tttacatttg cactcaatac
     9001 gtctctgtta aagttagcac tttttacgtt tacttctaat atatatggat ttattagaag
     9061 ggcctctttt atgaatctac tagcttcact ttctgtaaga ccttttgtat atttttgtcc
     9121 tattagttct gaaagctctg tcccatattc taaagagtat atctcatgtt catatctatt
     9181 tgtctttata cacttgtata tccaaacttt taaggcttct tttttttcaa ttattttaaa
     9241 gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc catgccactt cacgaaagat
     9301 tggcaattct tctgttttag gtaagatata atcctctggg acacctataa aaggaaatat
     9361 tgtactcatt ataagctcac caacttactt acaacagcaa atttttcgcc tattctaaac
     9421 attattacca tatccccaga ttcaaagtta tctataaaag gattttttac ttcatgctgg
     9481 tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat ccaactatct
     9541 atcaaaatat cttctttttc taatatgatg ttatttgtct ctatttttaa atctggtaat
     9601 ttacttttaa tttttccaat aaaaaaagaa ggttgattat gatactttcc ttcttctctt
     9661 attattccta taaattcatt tattggatta gccattatat caccatcctt ttataatact
     9721 ctcctagctg tattaaaatc tcttctacta cttaacttac ttattttaac tacatcacct
     9781 ttttgtggag catgtaaaaa ttctccatta cctatatata atccaacatg gctgactgga
     9841 ttatgaaaaa atactaaatc tcctgcttga agattttttt gttctacttt cttgcctttt
     9901 ttagattgtt gatttgacgt tcttggcaaa ctaacattaa cttttttaaa acagtagacc
     9961 attaaaccag aacagtcaaa actacttggt ccatttccac cccatttata aggtttacct
    10021 agatgttttt ttgcctctga tattactatt ttagctttat cagatacatt accagaataa
    10081 ccaccaataa taatttttcc ttttcttctt ccaaatttac tagcttcttc aacatttcca
    10141 aataaaatat ctatatggta tgttccattt ttttctatag ttattgcagg tcctacgtca
    10201 tttactttat agactccatc acgaccagaa actcctgtaa ttacctgtat ttcatctcca
    10261 tagttcaata aaggatgttt atttaaaaac tcttttgtat aataagtttg ctcatattta
    10321 ccaaccatag gagcagcgca agttttttta gatgggtcaa gttttttctt tctacaatct
    10381 gtatctccac cttcttcttt tctaggacag taagctgtaa attctgctgt aaactctttt
    10441 cctcctgcat aatcttctcc ccctaaatta ctttcttcct taggttcatc ctgtcctgct
    10501 gacttttcat ccataagatt ttgaaaatta agttcaagct caatttgata ttctccattt
    10561 tgccaagtat gtttgtctgt atctatataa aatagtccta caagctttgt ataagaatct
    10621 ttaactttta ctcctctacc agttatacaa cttacatctc cataaccttt aagagaacag
    10681 cttttttcta tcccattaaa ctcgctatca atatctacat cttgattttc ttgttgctga
    10741 attacttttt gcattattac atttacttcc ttaaaaattt cattatctat tttttcgcta
    10801 atcttgcttc catactggtc taccactatt actttatttt ttacattttc catgctctcc
    10861 gaaaaggtgg tatttataat attaaatccc tcttcaaaca taacacttaa tgtaacagtt
    10921 cctttttcaa taacattaaa cttatctaaa ttagcctcta tcatatactt tttctttgtc
    10981 tttttacttg cctctgtata tgcactcatt attgtgtcat aaccatttac tcctataaac
    11041 atctttgtat acttgacatt ggtctttgct attgttccaa ctgaaagcct attttcagca
    11101 aatacttgct ttgctatgtc ttcaactaac ttatctttaa aattgtatga cacttcactt
    11161 tgtgtaagta aaaatcccat atctttagat acaaaactaa tttcattact gcttgaatct
    11221 ttagacctat taattatcat tcctctaaag agttctttct catctacata gaaacagact
    11281 gtactagcta ttggtatatc gatttgttgg aaatttacat caaatgatga ttgtattatt
    11341 gaaaactcta gtgtccttga tggagattta taatcacctg accatgatac cttgtctact
    11401 atgtcagtta tgtcatatat acttccgttt tttatgtgta cccaaatctt aataataaaa
    11461 aatcacctgc cttttcattt aattctatca gcagttactt acaacttatt atctataata
    11521 gttattgttt tggagttact tttggtatct ttattctttt atactctttt aaatctaatg
    11581 taaaatatac atctcgagtc ccatctcttt cttcataact aaacccttca attatgactt
    11641 ccatatttat atttgtttcc gtaattgtaa atcttaatat aaaaccttcc ttcatccatt
    11701 tttctatctt atttacacaa tcatatggtg atggaaaacc tgtatagtca cagaaagtgt
    11761 agtctttatt tggaaaaaaa cttgatattg aaatgctttt aagagctaca ccaccaaaag
    11821 ttgctacttc acctattttg agtatactag aagagtttac agcagctttt ccattaatac
    11881 taaaggaaga tggaaatact ggaaatctaa atctatcttc tgcttgtcta agccacattt
    11941 ccattataag acacccccta actgtgccaa ttggatactt gcaactaatt ttgatgttat
    12001 cttttctata tcagcttctt ctctaatcac tattgtatca gctaacttag ctatgtttat
    12061 atttccacca ttttttctgc ttctatattg atttgcttct tgtttagtta aaaccatttc
    12121 accttcatgg agtctggttt gatagtcatt ataaggaaca tagttcaatc caaatgcatt
    12181 attacctttc ttacttgaat aagctttacc agaatcacca gcctttgttc cagcatatgg
    12241 attatctttg taattttttt tactcttttc cactagattt aaaactgttc ctgtgatgct
    12301 tccatttttt atcttattaa tcatagtttg ccaccatttg catatatctt taactatttt
    12361 acatacttta tctaatatgt taaaaaaagc tcccaatatt ggctctataa ttttccatcc
    12421 agtttccaac agaggtccta aggtctccca tacagttttc caaataacac ccagtgtctc
    12481 tataaaattg tttatttgag gtgcatattc acctaaaaaa gtaaaaattc ttgtgataat
    12541 aggactaaca gcttcaacta caggtccgat tagttggaat gctgtagtta ctattgtacc
    12601 aaatatactt attaatcctt gtgcaattgg tgatttattg tttatactat ctagtaaatt
    12661 atttactaag tttactactg gaagtagtga atctattaat gctgtaaaat caatattttg
    12721 taatagtgta cttcctgcgt taacaattgc ttttggtgca ttccctatca ttgttgctgc
    12781 atgattttca aaacgttctt gcttattttc aaccatcatt ttacgctttt cagcctcatt
    12841 agaaattcga tattgagtag aatcagaaaa ctcttttcca ttttttacac ttctagattg
    12901 catctggtca tatttgttgc caataattat cttaccattc attacattgc ctattttacc
    12961 agccattttt tcagataagc cagctaaatt ccctaatcca ctttctataa aagtttttat
    13021 tttctttagt ccttgaccta aaatatcatc aaaaggttta acaatactct tcaaaccatt
    13081 gttaatgctt gtttgagctc ctgatattat acctggtatt gtattactca tttctttagc
    13141 taaccccatc tgctttacac ttatgccttc caatgcttca tcaaaagttt tataattttc
    13201 tcctaccaga gtattaagag attccatatt tccttttgct gcacttaaga aagctgctga
    13261 tacatcttgt tcactacttg tattcatatt taaagctcta acatctacca tagcttgagt
    13321 tatatctttt tcttttttgc caccagtaat attactaatc attttagctt tagttcccat
    13381 tgcaacaaca tcttcagggt caaaaggcgt atcatttgcc atatttacta aactgtcata
    13441 atacttatca acatcttttt ggctagtttt tccagtattt cttttcatta cactttctag
    13501 aacaactttc tgattttgtt cctgaccaag taagtcaaca ccctttttca cagcgacacc
    13561 tatagtagca ccatttgtca aatcagatat cttccctttt atcgaaccaa gtattccact
    13621 tgccatatcc tttacagcta ttgcaccact ccatacctta cctgcaaaca atctcaattt
    13681 gggaaatact ttacttataa tgctacttgc catatctttt atagctatta caccactcca
    13741 aaaagtattt ttaaacaaca ttaacttagg caaaattcta gccatagcac tacttaccat
    13801 atctttaaga ttaatacttc cttgataagg acgtttctct actcgtttta attttcccaa
    13861 gatactactt atcactctct tagtcatatc ttttgctttt attacgatat tccaaatctt
    13921 gccaccaatc ttttttaaat ttccaagtaa tttattttta gttttttcta cttcatcact
    13981 tttagcttta aatattaata taatcataat atttgaatta aactcatttt tcttttgaat
    14041 caacttatta aaataatcaa taatcatagc tatagttatt gctagtctca taatacttcc
    14101 atttaaactt ccaataactc ctgaaacagc atttatgcta gaacttaatt ttgccatagt
    14161 tgaacttaca ctactagcag tagtttgaac ttttcttaac gaggaattta aactgtttac
    14221 tctactactt gcacttccaa tagaattttt tatgctacta gccattacat tagtggaaag
    14281 tgaaacagcc acagttgctc tcctaaaatt atcagttact gatataattt ggttcatttt
    14341 tggtgtgtaa ttatctgtaa gatacatatt tataccttct tctctcacat ttcccattac
    14401 ataccacctc ctgtcaaagg acacactcca tatccatgtt tcattttttc aacttcttcc
    14461 tcaatttcta aggcgaaaaa agcttcaata agctttaatt cgcctttatc cattgcataa
    14521 aaaagggacg gtcttatacc ttttttcttc caataatagt acattattgt agtcttggta
    14581 tccgtcccta ttagtttttt acctcttcta tcactgcatt tttaccataa cccataacat
    14641 cttgaagtat tctatatagg ttggtaattt cacctggtaa taatagtttt cttgccaatt
    14701 cttttggtgt tggagcctta aatttatcca ttagttcctt atttttaaat aaaagctttc
    14761 catccaaatc acaaacacct tctataattg taagcatctg catctccggt aagtctatat
    14821 ccgttttcat attttcatca attttaagac aagtattttg tatctcttca tctttactta
    14881 ttgttaaagc tctacacatt actttaaatt cctgcccaaa tatagttgat aatctcttaa
    14941 gttcaactat agtacttggt ctttctaagt tttctgtatc tgcatttaaa agtaaatcta
    15001 ctatgtttat attttttgat aatccatttt cattcattat tatttgctcc tttatcaatc
    15061 aaattcaatc aactaacttt tttacttatt aactacctaa atttattaag tatatatcta
    15121 ctaaatatca gtataaaacc aaatataaaa ctaaattata tcaagaaaat catactcagt
    15181 aaatgtgaaa ggtgcttcta tctctccttt tactccaacc tcccaatcag ctaaagttaa
    15241 atcatcaaat gctatatttt ttactgctat tctttctgct ccataagaat ctgggtcatt
    15301 taatttagat atagcaacaa atctaggttc agaacctctc tttatctttt ctccaattaa
    15361 cttgtgcatt cttgaactaa catggtatag agttattgaa ccttttcctt tatatcccat
    15421 atactttgta tcagtaccca tttgacctgc tattataata tcctcttttg taaattccat
    15481 ctttgcttga aacttcttta cttctgctac tttgtttcca tcaagccaaa gttctcccca
    15541 agtaccactc attacatttc tagcttccat atttgccata ttaataatcc tcctatattt
    15601 ctattgataa atctatatct tccatagcat caagtacttt tatttttgct tttaaaaata
    15661 ctttagaacc tgtgttagct tcttttattt cttgtaatgt catataagat aaatctactc
    15721 cttttgattt taaatacgat ttttgtgctt caaaatctat ttcaacagta gaatcagatt
    15781 ctataagtgc tgacttttct aattcttcta aataactttt tatagctact atcaataaac
    15841 atttgttgtc ataactgtta gtaacctttc ctatatagtc atctattatc acctttctta
    15901 tgtcactatg tataatatct aaagtgtcaa ctatttttat tttctggaac atttctcctt
    15961 tttcttctgt taactcagtt aaagaattta ctcctcttgc aattcttata gcccctgctt
    16021 ccttaataag tataagctct cctttattaa cccttgattc tgcatcaact ttcgtcatct
    16081 taggtatatc aactacatca ctaagcttag tataagttac tgattgactt aaaggtgttc
    16141 ctgctataag tccagccacc ctacttgtaa actcatcaac actgtatttc ttttctccaa
    16201 ctaacacatc ttctgtagta aaattaatta tcccttcatg atttcctaca acttttccta
    16261 atacagcttt aaccttaacc ttatctatat ctctaagttt aattatccaa ttttttatag
    16321 cagtcttatc agcttctact gcttttggca tacatagata attaaattcc ttagtctcta
    16381 aaaaatctaa tgcagtttga atatctgctt ctccttctat tacataaact aataatttat
    16441 ttggagtgtt aacatttccc atcaaagcta aatttatata ctctttattt tcagcagata
    16501 aatcaactgg tatatcctct ttttcatgta tttcattaag acctagtgcc ttagcatctt
    16561 taagcaccat tgcaattatt cctctagctg aacgttcttt aacagttgta gctagctcct
    16621 taaatgatat gttgatactt ggtaatccta tagccatact atcactctcc taaatttaaa
    16681 ttgatttctt ccatattttc atatactaca tcttcttcat atacactatc cagataattt
    16741 agtgtaattg taaagttcaa tacataaatt gactcttctt ttttaatact ttgttctagc
    16801 tttgttatat ttaattttct gtctgttacc tttatatttc tgttgaataa cttatttaat
    16861 tcatctgaaa tttcatacat tctaatactc tttgactgtg gttttggtaa gtattttata
    16921 gagataacaa tatctttatt attcgtattt aacattatag gtgtacaaat aactgatagt
    16981 atacttacaa aaaaacaaga cccttcagag ccttgcacac tttcatcatc aataaaaata
    17041 ctttcagtaa agtttttttt gagaatctca ataattgttt ctaatatttc cttgtattta
    17101 agcaaataat caccacacta tcactaaata ttcttgctta atctgataat cttactataa
    17161 atgatataag tcatttttaa atatacatct catttattcc tgtcaaatat cttctatatt
    17221 tttaggtaat attattcttt tcaatttatc acctccttca atatggattt ctttcacaat
    17281 aacattatct cacatatttt attgcctagt gttgcaatag tgtatataaa gtgttgtaaa
    17341 gttgtcatat tttttataat atctcactca atttaaatat catttttttc tttaaattgt
    17401 agcaagatgt tctatctaag tgtagtttca tagaaatata tgtcatattg ttttttgtct
    17461 tactattata aaaaagatta aaaaattcca tttctatatc atttagacat gttagagcat
    17521 tttctatctt ctctttttct atttctatat cttctttttc catttttaat tttcttaatc
    17581 tttcctctct ttttaacacc tcattttcca cagaagaact tatgttatat gtgttactag
    17641 tcttttctgt atataccatt gctccacatc cagagtattc attctcacat ctttttatgt
    17701 ccaaattaat attctttatc tttatatcca aatacttata attatgtaat ctttcatctg
    17761 tttctttaaa taatatatct tttttgtttt ccatagtact tctccttttt tatatactta
    17821 ttattagctt caaatttaca ataatctctt tagtgcacag ttttttacta atctcacaac
    17881 tttcaagaac atattgttct ataactgtat aaaaaaaact tcttctatat tttgttctaa
    17941 aatactagaa atcttcttag ccataagaag accaggattt tttatacctc tttctatttg
    18001 agatatatag tcatttgtaa ttccagcctt tctggcaagt tgaatttgtg tcataccttt
    18061 gcattttcta taatatttca agttattttt aaacattgtt ttatacctcc attaccatta
    18121 agaatatttt gttcttccac acttagatta tatagaatca tttgttcttt atcaatagat
    18181 tttagaaaaa aatattcttt tatattttat tagttttatt ttttagaaca atttgttata
    18241 atctatacat aaggtggtgt tataatgttt gccaaaagat taagagaact tagaaaggaa
    18301 tttggattga ctcaaagaga acttggagaa aaagtaggcg tttcccaaag agtcttaggg
    18361 tactatgaaa cagaaaacag atttcctgat gaacacattt taaacaaatt ggctgatgta
    18421 tttaatgtat cagttgatta ccttcttgga agaaccttag ttaaggaaaa tattgataca
    18481 gtagctgcac atagaaaaaa tccacatgaa gagttacctg aagaagcaca agaacaactt
    18541 aatgattata tagaattttt actaaataaa tataaaaaaa aataaaccat atgatgagca
    18601 gtctattttt ctgctctttt aatataattt aaaggcaaac atacattcta ttttaggggg
    18661 atttttatga ataatttgga taagcttttt gaattagctt ctcaagaaga gataataatt
    18721 cattatacca cttatattgc aggtgattta gaagggctat atataaacaa acatggtatt
    18781 aaaattatat cattactcag taatttaaaa caaaactcaa aaaaattgac atccatctta
    18841 gctgaagaac ttggacatca tttcactagc cttggatact atgtatcctc ttataacgat
    18901 tattatacaa aaattattat agataagtgc gaaaataaag ccttaaaatg ggcttgtgag
    18961 ttcttaatta ctgaagaaga tataataaac ataattaatt ctggcatcac ttgcgtttat
    19021 gaaatggcag acatacttaa tgtggatatt actttctttc aaaaaagatt agaattttta
    19081 tcattaaaaa aacagtcttt acaacttgga aataataaat atttaatatt aactaatttg
    19141 ccgtatttct acatatttga tccaatttca taataaaaat agtagtaatt ttaactggct
    19201 taacagtaaa atctatatat ttattttaac aaattttggt ttctataaat tcaatattaa
    19261 tatataataa tgatatcata ttatttaaaa ttctaaaaac aaatatatgc ttttgttaag
    19321 aaaggttgtt tgatgaatgt ataaagaaaa gttgtttgat aattatttta agttcttagc
    19381 actattattt tggccaatta tgtggtataa gtggatagta atttcaaatg gtactcttga
    19441 aaatatgtta tttaccatat atgcaatcgt cgcaattatt ttcatcattt tatattcggt
    19501 gtttatgata aaatataaag acattacaca aatagatttc ttttacagga taagtactct
    19561 acttgctttt atatttacac tttttagctt tttaatatac ccaaagagtt tatttttctt
    19621 gtatttaaag attattttta ctggaatata cctttattat tcaatagtaa aaactcttaa
    19681 atttaaagat gatgaaggtg tagtgggtat tatgagctca cttttattaa ttgtaataac
    19741 tttgttctac taaatttaat tctatttgac ttaatatggc tttaaatatt agacatctac
    19801 attattttct gatattattt aagatggtag tcttatatta ataatgaaat acactatgaa
    19861 ggagttaact attatggaaa aaaaagtttt agcaacagtt ggagaaaaag agataactaa
    19921 tattgatatt gaaaatgcat taaaatcatt agacccttat caagctatgc aatttaaaac
    19981 tgaagaagga aaaaaacact tattaaatga tttagtgaat caagaattat tcttcttaga
    20041 tgctaaagaa gaaaaattag atgaagaaga aatatttaaa ttagaaatga aaaagataga
    20101 agaaaatgta ttaaagcaat ttgctataaa taaagtttta tctagtgtta atgtaacaga
    20161 agatgaaaaa gtaaaattct ttgaagctaa taagagctct ttctctaaac cagaaagtgc
    20221 aactgctaaa catatattag tagatagtga tgaaaaagct aaagaaatac ttgctcaaat
    20281 aaaatcagaa gaaatatctt ttgaagatgc agctcttaaa cattcttctt gcccatctaa
    20341 agatatgggt ggtgatttag gaacattcgg tagaggacaa atggttcctg aatttgaaga
    20401 agctgtgttc tctatggcta aaggtgaagt aagtgaacca gttaaaactc aatttggata
    20461 ccatataata aaattagaag accttcaaga aagtacagag tctacatttg atgaagttaa
    20521 ggctgaagtt gaaaaaagtt tattatatca aaaacaaaat gaagtttatg gaaataagat
    20581 aaatgctctt aatgctaagt acggaaacct tgtaaaatac aacgattaat cataaggtca
    20641 tatataaaaa agctgtcttt tcacaatatt tattagaaaa gacagctttt tatattaaag
    20701 tttaaatatt cagtttcata ttatcattct tttattgtaa agaactcagc ttaaaagttt
    20761 aaataagatt tttaattatc attagctaaa tttttatatc cacgtattat ctcataacag
    20821 taacctatta catctcttct ttttattatc ccaataaatg tgtcttggtc atctataaca
    20881 ggaatgaaat tttgatttaa agattttata actaaatctt caatatctgc gttgatggaa
    20941 acaggagaat tatccatttt tctatttata tctgtcactg gaacatcttc caatcccttt
    21001 aagtccagat ttaaatcgtt ttttaatgtc cataaaaaat caccttctgt tatagttcca
    21061 acatatttac catccttact tattattgga attgcagagt atttgtgata ttccattttt
    21121 tctagtgctt gccttatagt gtagtcttca tatatatatg ctacttcact ttttggtgtt
    21181 agaaagaata atatgttcat tacactaccc accgttactg aaaacttata aatattactc
    21241 aatatttata atgttacttt tttgcagtaa ctttctcctt tcttattttc taagtattct
    21301 taatactata cgctgtaaca agcgtttttc ctttgctcat tttaatctta ttatgcttta
    21361 ttaatatttt tatatggtct atcagtatca cttattctct ttttagaaaa tttactaaaa
    21421 attaattgac ttaaacttag ccataataat tttactctac actattaatt ttaacatatt
    21481 tatctcatta ttaatgattt tattttaata attttaattt attttgctat taattttatt
    21541 tacaaaaact gaaaaaaccg ctaaatgatt agcggcttat ctctcatatt actagaacac
    21601 taatatatat gaatttttaa ttataatcta actacgttag cagcttgagg acctttagct
    21661 ccatctacta cttcgaactc aacgctttgt ccttcttcta atgttttgta tccatctcca
    21721 gttatagctg agaaatgtac gaaaacatct ccttcacctt ctacagatat gaatccaaat
    21781 cctttttcgt tgttaaacca tttaactaca ccagttttca ttaataaatc ctcctaaaaa
    21841 aacacaaata tttagctact cttttctgta accttattac tactctatca tatatagcta
    21901 tgtttgtcaa ttattattat ttttatacat taataatcaa tttttttact ttataatttt
    21961 aaatgattat tatcttttac actttatttt tctttataat tttttaataa tttattttta
    22021 ttatatggaa tcttatggac ttatcactcc agcctaaatc ccatggcacc aacattctat
    22081 cagtattatg gcaagttacc aaaggatatc ctcttgagtc taatcctgtc actgtcgaaa
    22141 catgtgttat tctgcctttc ttctcataac caacaaaatc ccccggacgt aaattataag
    22201 cttctttgta tacttcttca taggaacctt tagcaattaa tgaacctctt ccactataca
    22261 gtgcataatt tttaaaagct tgtgcattta cccatgcttt tgtacctgct ctctcgctat
    22321 aattccaaat tgagttcttt ttgaaccttc cactttcaaa cattatctgt gatgcaaagt
    22381 ttgcacaatc tccaccttct ggattataat ccctatagtt tggattaaat ttcataccat
    22441 gttcttcatc agctgcggct ccacagtatt tatgtgcata atctatagcc tttttttgtt
    22501 catctgtaag ctgtaactct acaccatctt gttgcccaat atagtttctt atatcctcag
    22561 attttatatt ttctaaattt aaagagtctg caaaagggtc tgtgtaccac tctttagtta
    22621 tgacatattg attatctata atttttacat ttagataatg atatgtacct attctaaagg
    22681 cattttcttt gtccttttga ttttcatatg aatacttata ttcagtcgat actgaacaga
    22741 ttataccata gaggtctttt tctttttctt ttattttttt aatcttaacc cttgtcttta
    22801 tgtctttaaa ttttacacct tgcttactag accagttttc taaatacttc atctttttta
    22861 cttcattttc ataagcccac agaccaaatt ttttatccgt ctcataaaga ccttttaaaa
    22921 tgttttcatt tttctctaat attgccttat ttctgtagtc aaataagttt tcaagcaata
    22981 gtttatattg gtcttttaca tcatcacctt caacatttgc agattccatg actttttctt
    23041 ccatattttt catacttaaa tcattgatta aaaatacacc tgctgtagta gctaaagaca
    23101 aaaatacaaa tgccattata cctttgatgt acttattctt atttttcagt attttcaaag
    23161 ctacacctcc caaatagtac accttataaa tgaaattaca aagaagattc tgtcatatac
    23221 aataattttc atatttaata attctctatt ttattatgtc caatattaaa aattaattgt
    23281 atcaatacac tgtttttttt acaatttttt attttataat ctactttttt ctttttttta
    23341 tagtaatata tatgtatata aatattttgg tatcattata attaatacat actaatggag
    23401 ggttttttat gcaaaaaaaa gtcgcagcaa ttaatgactt atctggaata ggaaaatgct
    23461 cattatcggt tgctatacct atactttcag cattaaaggt tcaatgttgt ccatttccaa
    23521 cggccatact ttcaagtcaa actggttatc ctgaatttac tttcttagac tttactgatg
    23581 aaatggtaaa atattcaaat gtttggaaaa atttaaaagt caattttgat agtatatata
    23641 gtggtttttt gggttcaaag catcaaattg agatagtggc taattttata aatgattatc
    23701 ccaatgcttt tatcgtagtt gaccctgtga tgggtgataa tggagttatg tatccaatat
    23761 tcacagaaga aatgagacag gaaattaaag aattagttaa acattcagat ttaactactc
    23821 ctaatttaac agaagcttgt ttcttaactg gaaatgatta tactaaaagc gattataata
    23881 gagatgagct tatatatatt gcaaaatctg tatctgattt gggacctagc aaagtcgtta
    23941 ttactggtat attagaagat gataatatat taaatttggc ttatgataga gataatgacc
    24001 atgtattctt tacatcagtc aaatacaata attgttctta cagtggaact ggagatatat
    24061 tcacatctat actttgtggt atgttagtaa ataaacatga tttgggagta gcggttaata
    24121 ctgcaacaga ttttatatat aaaactataa attacacatc tcaatttgat actgatagaa
    24181 atgatggagt tatgtttgaa aactttttat cagatttaac taatatataa aatcaaaaaa
    24241 actatccatt ttataaatac ctataaatca taaaatttag gtatttacaa aatggatatt
    24301 atttactaaa ctattggtat ttcaattgtt accatagcac caccagtttt cacagaattt
    24361 tcaatatata aaattccatt atgtttttgt acaatattat ttacaatata aagtcccatt
    24421 ccgtaatgag atttagaatt tctactcaaa tctcctgtat aaaactgttc tgtagctttt
    24481 gacaaatctg ctttagaaaa accacttcca caatctgtaa ctacaaattt taagtattta
    24541 ttacttactg atacacttat gtatagttta ctctggcttg gtgaataatc tatagcattt
    24601 gaaattatat tcataatagc tctgaataac agttctctat ctatagttat atttttaggc
    24661 aaattatctt gtttataaaa ctccacatct aagtttttag tactagctaa tgcttctatt
    24721 tgcagtaaga catcttttat aaactcactc atattaattg cttctttatt taatatatag
    24781 ccattctctg tttttgatat atctattaaa gtattaatat atttttctat ttgagtgaca
    24841 ccctttgaga tatattccat gtattctaat aactcaggat tttcatttat ttcaattaat
    24901 aagtcagtat ttccatgtat aattgttaat ggtgttttta tatcatgtgc cagtgcagat
    24961 atctgttcct ttctattttc ttctagcatc cattgttttt ctaaagaatt ctttaacgcc
    25021 aacttcattt tatccataga aaaaaggata ttgttaattt cataaatttt actagattct
    25081 acaacaaaat ctaaatcctg ttgttctatt ttttctgtag tattttttaa aagctccatt
    25141 tcaatattaa atttcttgcc aaatactttt gataatataa caatttcaat aaaaaatatt
    25201 atactaaaaa taatcatacc taacatctct ggttttggta aatatgctct taaaactgga
    25261 gaactatact ctgatactag tttgtatttt attataaaaa cttcatcttt cttaaaaaac
    25321 tttatataac tttctgaata tgcatgcctt tcttcaatat ctctcattaa attccatact
    25381 tgttttgatt cttttttatt aaaagtgcct gagatcaaat tcccatctaa agtataaaca
    25441 ccatactcgt ataaatttgg aattatatct tcaattgctt tttcacttga aataatcttt
    25501 tctttatatt tatcaaattg tttttctgaa taattagctg gtaatattac acctgatgat
    25561 aataatacag aaaatgataa cattaaaaga agtgcaaatg aaattgtcat aacaaagaat
    25621 aaagataaat attttataaa aatagttctt aacttttcaa cttttttgtt gttagatttt
    25681 ttacttctaa gcatttgtgc catatcctcc aaatatttta attaattcat aattaatacc
    25741 taaccagttt ttatttgatt attttatatt tttcattgct aagttatatt ttaacataat
    25801 aatataaata taatgtaaat aaacagtcaa tttttattta ttcttctgac tttttacctt
    25861 cccatttaga aaaccaaata cataaaatta caaatgctaa aattgttaca aaaccacaca
    25921 ttaaacctgt tgattgatat gcttttattg attcaacctt tatatattca accgttttag
    25981 aacttgaaaa agacgaccaa atagttataa atctaacacc ccatgcacaa ggaaaatact
    26041 gccaaatgcc agaacccaat ccagttaata ataatgctga aagtaacatt tcaactatac
    26101 ctactccaat tgatgttcct tttccgaacc ttaaacttaa gaataaatgt aatatgtatt
    26161 caaataaatt acacccaatt aaaattagaa ttgaaatcat ataaaagctt gatgtaaagt
    26221 aagccttaga tataccaaat ttaaaaccta atacagacac taatgttgct acaaaaccaa
    26281 gacatactaa gattatatat ttacttataa gagtaagata ttttggatta gatgttgtta
    26341 acatatgttt ataattccct gcataatatt cttgttcaac aaacattgaa cacacaacac
    26401 ctgacaataa aggaaaagct attgctataa cttgtaaata tgctactatc tttaatatat
    26461 tatcagctcc tgaaacctta taataccata aaaacaatcc catccccatt aaagcaatgc
    26521 aaatgtgcat tagtattata aaatttctct ttaattttat taaatcagaa caaaatattc
    26581 ttattagttg tctcatatta cttcacctct ctattattga accaagctct tgtaactaaa
    26641 gcaaataata caaataaaat aacagatatt aatactcctg gtaaaataac agcattattt
    26701 aaaagttcac tcccctctgg tacaggaagc ccatttggta gaatatgcaa tacaacaacc
    26761 attaatcttg aaggaatcga atatggaaat acataccaca tttctgtagt tgagaaaaca
    26821 gcaactccaa caatagtaaa gaacatattt gttagaactg taacaaatat accaaatttt
    26881 gaagctaaaa acatacaaag aggaatctgc cacataaata gtaaaataat taatatagtt
    26941 gcaatgatat tttgacctat tgaaatagtt tctttataga ttatcccaac aaaaactaca
    27001 cctattgcag aaataatcga tgctatagaa caacaaataa tacagtaaat catttttcca
    27061 gcccaaacgt ctttcatttc aattggcaat ggtaaaatac cacgatattt acttttttca
    27121 tcttttaata ctattaaaga agatattaat gttaatgctc ctggaagaaa tgtcgtatac
    27181 caccagttga aagcagcatt ttgccctcca cctagtccac acaaaagtat acaaaacact
    27241 ggaactatcc atatcaactt cattccaaaa gttcctttca tcttttgaaa ttccactcta
    27301 ataaatcttt ccatattact cccctctctt tctattttca gctacaactt tcataaaaag
    27361 tttttctaag ttttgttctt ttgatacttc tccttgataa ccaattactc cattagaaat
    27421 tattccgatt tcatctacga cttgttcaac ttcagacaag atatggcttg ataaaataac
    27481 tgtaattcct tgttttggaa aagaacgaat caattttcta agttcttcaa taccaatagg
    27541 gtcaagacca tttgtaggct cgtctaagat taaaagtttt gggtgattta ataatgcaat
    27601 agcaatacca agacgttgct tcatacctag agagaattga cctgaacgct ttttaaaagt
    27661 atctcttaag tcgacagtct ctaaaacttc ttcaattcga gagtcaggaa gaccaagtaa
    27721 agtagtatgg acttttaaat tttctcgtgc tgttaaattc tcatatatag caggagactc
    27781 aataagagaa ccaatatttg ataaatcctt tctagtccat ctatgtccct caaacattat
    27841 ttttcctgaa gttggattta aaatcccagt tatcattttt aataaagtag atttcccagc
    27901 tccatttggt cctaataatc catatataga attagattta attgctagtg aaatattatc
    27961 aactgccatc tgctccttaa attttttaga aactcctttt acctgcaaaa tataattatt
    28021 cataattttc atcctttctt ttgtcaaact tatttacaag aatcattcta aatcataaat
    28081 ataaggattt tataagaatt aaaaaacaaa aaaaagatgt gttaaaaaca catctttttt
    28141 tatcttaatt ctattaattt tccattgtag tatatctcta cttcttcaat ccaaatgcta
    28201 ttatcttctt cttggatgac atttttaata atattattta tagtatatat tatattttgt
    28261 tcattcaaat aatcatcttt ccaagaaact ctactttttt tatctggttc tatcaagttt
    28321 attttaacaa gattatcttt ttccttaact tctacttcca tgtctaaacc attaaaatac
    28381 tcatttgata atgtatctaa gattaatcgc agtttatcct taagtgaact ttcttcatca
    28441 atctgtaagt actttactat tttctcatcc tttgaaatta tctctatagg tattttagta
    28501 gtttttttag aattaatcat tttactagtt gtttcttctt ttgcatcttc atccttcttt
    28561 acaactgctg attctttaga atcaactagc tcaactgcac tgatgctata atccatacat
    28621 gctgtactca ataaagtagt agcaattatt aaaaaactta ctgctatttt attcataagt
    28681 atccctctcc taattctctt tcgatactta ttataccata gtatagatta tatttttata
    28741 cttttaaatc aatttataaa ttattatagt aaattattta caattctaag ctatttgaat
    28801 ttatttttat agtatattct ttctctaaat tcgacagatt tttattcaaa agccctttta
    28861 tttccctcgt atttctatac ttgttgctat gttcttcact atgttcataa tcatatgaat
    28921 cattgtcata ttcttcatca ttattaaatt cttcaattgc ttcttttgac tttatatcac
    28981 aattaaattt atacttattt actggtatat caagtcttac cttagaattt ggaacttcta
    29041 tgtccaggaa cttagcaatt ggcatattag agtacaagta aaattcactt tcataatttg
    29101 aatttctacc tattttctct tttatattta cattttcagg tatatactct tctatattat
    29161 tgataaatac atcttcattc tctgaagata tatccaatga ctctgattct atgtttacat
    29221 ttttaacgcc taaaattgat tttgttgaca agtgaagatt gctattagat attaagttta
    29281 ggttttctaa tctatttact ttgttctctg ttatagctct actaaaacct ccataagaag
    29341 ttttatatgt tatagtattt gaagctacat tatcatatac aaataagtat gaacttgtac
    29401 ttgatgcatt tatgtctaca ttatttggta catatattat aatttctcta taatcatgtg
    29461 aaaacatact atttatagat gtatttaata attggtcgaa atttttaatc tttttaattt
    29521 tattttcata ttttctttca ccttttacaa ctaaagtctt atcttttaaa gttacattgt
    29581 atttgtaaaa ctcattattt cctcttgttg ttacacgcac atcttcactt gaacttttct
    29641 ttattgtaac gtttatattg tcaacagata tatctaactt gtcaatattt actttttctt
    29701 gataaagttt attttctttt ttaaattcag cttccttttc taaaccaaag tttattaggc
    29761 ttggcataac taaaatccct gaccatgttg tccctattat acctattacc aataaaactg
    29821 cagcaaatat agcaagtttt ttattcatgt ttatcttctc ctttcttatt ttctatatct
    29881 tcaaggtcct tatatatcgc ctcaaaattt atactatcat cattatattc atcagtttct
    29941 tttatgcttt cttcttctct attgtagtaa tcattattat atgaatcttc attaatgtct
    30001 ttattcaaat cttcatcatt taaagtagct ttttttcttt tttccatctt ttcatttgcc
    30061 tttatataaa tattttttct ctttatccaa tgtatataat tatttagtaa ttgtttacct
    30121 agttttatag caaataataa aatttgccat gcaagtatct caagccctac aaatgctatg
    30181 gttgcgaaaa tacaaaacat cataacatct ggaaccattg ctataaattt aatcacaaat
    30241 ggaattaata taacaaaagc aattaatgaa catactaatg caatagtaac agatacaata
    30301 gcacatacaa acgcaaatgc tggaacaatt agaaataaag ataacactac taataatccc
    30361 tttataactt gtctagataa tccccctaca ccttcttcta tacttggtgt aagtttatca
    30421 tttatatttt cttttgattt attgtacttt tccttagctt catctttcaa gatagagtaa
    30481 tcctttttta tatttgattt aaacctacta aatttaccat ttaatatatc tgtattcatt
    30541 atttttccat ctatattttc ttttgattta ctatactttt cttttgcttc atcttttaag
    30601 atagagtaac cttttttcat atctgattta aatttactaa acttatcatt caaaacatct
    30661 gtatttatta ttttttcctc ttcaatattt ttttctttag tctgcaagat aagttctttg
    30721 gctatagatt ttggtgaacc aagtgatgct attatctcca tatcactctt tccctctata
    30781 agaccatcta cgaaatactc ttcataatct ctcaatatat cattaacttc gctctcagaa
    30841 aaatcttttt ttagataatc ctttaaaatt tcaagaaact cagttttatt caagttcatt
    30901 acctccctca tcttcactta ttaaaatctc aacagccttt acaaattctc tccactcttc
    30961 catcagattg gataaattct cttttcctag tgaagttatt ctatagtatt ttctagcagg
    31021 accttcatta gattctagta tatatgtttc gaaatacaaa tcttttgtaa gtcttcttag
    31081 aataggatat attgttcctt catttacatc tattacttta gagatgttat gtactatctc
    31141 atatccatac atatctttct tagatatgag agcaagtaca caaatttcta aaacaccctt
    31201 tctaaattgt gtgttcatat actccatcct ttctttaaat acacagtatt gtgttttaca
    31261 aggtactatg ttaaaacttc ttatatcctc ataataacac agtactatgt tctgcacaat
    31321 agttattttt aaatattctt gttgattaaa tccttctata aatgtataat actctaatca
    31381 gatatatttt atgaaagtgg tgataaattg gctaaaatga aagatgataa aacaatatta
    31441 atcttaactg ctcaatttgg tgctggacat ataagtgctg caaaagcagt caaagaatgt
    31501 attattgaaa aatatagtaa ctataatgtt gttatacaaa actttataaa tgcaagtatt
    31561 cccatgatga ataaacctat ggtaaaactt tatgaaaaca atacaaaata tactcctgga
    31621 ttatataatt actattatta ctttaagaaa tcgtttgatt caagacatga tttatctcat
    31681 aaattatata cacctaaact ttctgagtat attgctgata taaatccaga tttgattatc
    31741 tctacatttc cactagcggc tgcttgtgta aataacttta agataaaaaa tcctgatata
    31801 aatataccta cacttacagt tataacagat gttgtagata gtatggaatg ggtttttgaa
    31861 aatacagatt tgtattttgt tccttctcct gaaataaaaa atagattttt tcagaaagga
    31921 ataaatccag actccataaa ggttactgga gttcctgttg ataagagatt tcaaattgaa
    31981 agtaaagaac tatgttgtga taaatataga ctattacttc taggtggagg aagaggccta
    32041 tttgatatag atgaggattt tatgcattgg atagatgaat ttattgaaga acatagtaat
    32101 tctatagaaa ttacaatagt tactggtaaa aacaaaaaat tatatgacaa tctaactcat
    32161 aaaaaacctc taaaaaatat taaagtactt ggatttgtaa acgacatgta taatctaata
    32221 agagaatgtg acctaatgct aactaagcct ggaggagcaa ctatatttga agctattcaa
    32281 tcacaaactc cagtactcgt gaaaatgcca aaagttggac aagaaattga aaacgctaaa
    32341 tttataatag ataagggtct tggcatgatt tatagtgatg acctagacct taaaaacata
    32401 ttttataaat tagtatcaaa tgaatttgat tctataataa actttatgaa aaaaaattta
    32461 gaagaattta aaacagttat tcatccagag aaaattgctg attatatatc agaattaata
    32521 gataatcatt acagttaaat tgtactatta aaaaatttca aacataaaat taaaggcaat
    32581 caagtatgct tttacacttg agaatttact catagtgttt aataatactt gattgccttt
    32641 atcatatcaa aatcaaattg ttatattagt atatattttt cttgtactgc atatacagtt
    32701 gttgtatttc ttccatactt ctctttaatt ctatttgtaa atctgaagca gtctggtaat
    32761 ccttattttc tatagcttct ctcactctag tgaatagtga ttctctagtt aaagtatcct
    32821 tcataatatt gaactttaaa tccgatattg agttccaaac tacctcatca agcgcatcca
    32881 tattttcttt tgtgtgtatc ttgacaatac ttcttatttt ttgaatccca gcctctatta
    32941 ttctagtttt taattttttt tgccacttat ctatagctcc aatcttaaat gattctatta
    33001 tactatcagt aaatacatca ccttgtttta aagattttaa ttttgaagca tatatttcta
    33061 agtttttcat attttcataa actgtagccg gtggtattcc aaatcttgca tttctttctt
    33121 ccaaactata atgttcaaat acatcatttt catctctata tgctctatct ttttctaaat
    33181 agaaactttc ttcacctaca tttttagata actctttttc aagttctttt gtactaagac
    33241 cactcttagc tgctgctttt ataccatcca acattgtttg ataacatcct gctattacta
    33301 aatacgtgtt agatagaggg cttggtgacc ttaactcaaa tcttacagtc tttggatttt
    33361 tcatatctct tattaatcca acaagcactg acctatttct tgatggaatc tcataactat
    33421 gccctaaaga tgtaactatg catacaggag cttcaaatcc aggaactaat ctattaaatc
    33481 catcattaga agctgttaca attgggttta atacttcata attgtaaaga agtcccataa
    33541 gagccccata tcctagttca cttagataat cttcttttaa atcttttgga gcaaatagat
    33601 tttttatact tccatccttt aacttcgcac ttactcctac atgtgtatgt ccaccacttc
    33661 ctgctactcc atgaattggc tttgctttaa atgtaacttc tagtccatgt gatgtaaata
    33721 catcctctat aacatctctt actaataatt cattgtctgc tgcttgtaat ggtgtagaaa
    33781 acttccaaga aacctctaat tgctccatag catggtttgt tcttccatct atactaatag
    33841 aactttgtat tcctccgacc tctttgtgag ccatttcagg gtttacacct agcttttgaa
    33901 gtataattaa acttttctct aaacaagttc ttataatacc atgagttctt ttccagtatt
    33961 gctcttttag gctttgtgat acatataatt tctctaagtc agctttatcc tcaggagtat
    34021 taacccagaa ctcaagttct gtagctgctg ttaacattat ttcctctata tcatctactg
    34081 aatctattcc tatgttattt attacatgtg gatattctct aaatatttca tacattgact
    34141 ttttaaagta cttatctgct ttttgaagta ctcctctaga acatactttt ttattatcat
    34201 gtattaaaaa tgcaggtatc tttaatgttc caacaggtag acctacttct tcgtctatat
    34261 attccatatt gtaatctaca taccaatgac aacttttatc tggcattaaa tctactttcg
    34321 cattatttaa tgtagctata ttataaagct caacacttga accatcagtc tgtatagctg
    34381 attccaaaaa tttatctata tcctccaaaa ataattctac aggaatcttt tcatctgttg
    34441 catttcctcc taaatcaacc cccattaagg aaacaaatct tatattttta ttgcttttta
    34501 ggatttctct taattcttca ttagtgtgtt tatctttctc tattacgtat aatagactat
    34561 ccatttttcc actctcccat aatttttatg tatattttat tttgttaaat acaagataaa
    34621 tttcattttc cttacattca tgaatttttc ttgcaaatta ttcgtgtttt attcgtgttt
    34681 ttatttatat tattatatat caagtataaa aagttcgcaa tattttttat tggaaaaaat
    34741 attatacata caatagttta acatttttac aaatatgggg tatactaaaa taaaagtatt
    34801 tttatttgaa aatttaattt tatttataat ataatctata gtaaaagaaa taataaaaaa
    34861 atttaggaga taaactttat ggaatacata attttcgatt tggaatttaa ccaaggattt
    34921 gataaaaaac tcaataaaac tgtatccaat gaaaagtgtc cttttgaaat tatacaaata
    34981 ggagcaatta aattggattc taaattcaat ataatagata cgtttaatag ctatgtaaaa
    35041 cctacaattt ataaagacat acatcccttt attagtcgta tgactaatat taaaaattct
    35101 gattttaatg attcgcctac tttccctgag gtctacaaca attttataaa atttatatca
    35161 tcacaagacc ctatattatg tgtttgggga gttggagatt taaaggaatt atacaggaat
    35221 atcaactatc acaagctacc atcaagttcc ttacctaaat cttatattaa tatacaacaa
    35281 catgcatcta aatatttcaa taatccagca ggaaaaagca ttggcctaca aaatgctatt
    35341 tctatattgg aacttgatga aaaaatgtct tatcacaatg ctttaaatga tgcatattat
    35401 actgctaaag tttttattaa gatatacaat ccaagtatag tcccagatat atatctttat
    35461 acgagtataa aacctaaaac tataagatat tctaataaaa aaagagttga ttatgataag
    35521 ttatttgatg aatttaggaa aattttaaat cgtgaattaa ctaaagacga gaaaaaaatc
    35581 attaacttag cttataatat ggggaaaaca aatcaattta cattagaaaa tgttaagcaa
    35641 agaaaaaaca aatagagttt atttagataa aaaataggca tgtagattaa aaaactagtt
    35701 aactttaaat ttatatctac agcccaattt ttatcgtaat taatttttat tttatctcta
    35761 attcaactgg acaatggtct gaacctaata tttcagtgtg tatatccgca cttactaatc
    35821 tatcttctaa ttttttggat gcacaaaaat aatctattct ccaccctgca ttattttttc
    35881 ttgcattaaa tctatatgac caccaactat aaacgccttc tttatcagga ttaaaatatc
    35941 tatatgtgtc tataaaccca ctgtctaata atttagaaaa cttctctctt tcttctttag
    36001 taaaacctgc atttttcata ttatttttag gattttttaa atctatttct ttatgtgcaa
    36061 cgttcatatc tccacacaca attacaggct tatgattatc tagtttggtt aaataatcta
    36121 taaagtcgtc ttcccatcta gttctatatt ctagtctctt taattcagat tgagaatttg
    36181 gcgtatatac tgtaacaaag taaaaatctt caaactctag agttatcact ctaccctctt
    36241 tgtcatgttc ttctatattt attccataca ttacttttaa aggctctttt tttgtaaata
    36301 tagcagtacc tgaataaccc tttctctcag cataattcca atattgaaaa tatcctggta
    36361 aatctaactc tatttgccct tcttgcaatt tagtttcttg taaacaaaat atatctgcat
    36421 ctacttcttt gaaaaagtct aaaaatcctt tcccaacaca agctcttatt ccattaacat
    36481 tccatgatat aaatttcata cttatactcc tcatctaaac attgacacac tccccatagc
    36541 taaagatggg ggcttttcaa caattttatt gtaaattctt aatttacttt tattttaaca
    36601 atactatact ataatttcat ctataattac tatttactta atacctaaaa atatatgttt
    36661 taatcaattt ttaacttttt tgttaaatct tcagggctca ttgaagaaac atacatattt
    36721 gttccaagtt caaatcctcc aaaattatat tttcttggga ttatgtgcat atgaacatta
    36781 aaatacgatt ttgtttcaat ctttgttgga tgagtgtgaa tacataagtt aaatggcata
    36841 tatccattga tattgtataa cttcttaaac aacttttcaa atattgtaga aagctcctgt
    36901 atattattat catttaactc accaaactct attttatctt taaatattat tcttacttct
    36961 ccactatact tggttgcata tggtataagt accaaaaaag cttctccatt ataaacaact
    37021 cttctatcta aacttatttc attttctatt aaatcatcat acaaagagct tttattgctg
    37081 tcataatact cctttgaaac taacagttca ttcgtaattt caggtggaat aaaagacata
    37141 gacaatattt gagcatgtgg atgcatcaaa gatgctcctg cctttctcaa gaagtttttg
    37201 aagatactaa catactctac tttatcatct ttcgacaaat ctctaaatct atttctatac
    37261 ataaaaaaaa catcttcaaa ttctttttga ctcatgttat aaaaactacg attatgttta
    37321 tcgctctcta tcattacttc atgaacacca tatacatctc ttgccatatc atcaattata
    37381 ggaaatttat tgtagacaga ttttacaagc catccttctt cactttctat ttcaaataat
    37441 gcatcagttg catgagcttc atttcctttg caaaatgggc attcttcatc atattcatta
    37501 gtttcttcat cttcatttgg gatatctgct gtatctaaag gtctttttaa cctatctgta
    37561 gcaaatataa caacatcatt agtaattgga tctattctta attctttcat aattaacacc
    37621 tcttagttaa tagcctttaa tataa
//
LOCUS       DAMKPG010000031        35414 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMKPG010000031 DAMKPG010000000
VERSION     DAMKPG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.034"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            56..1222
                     /locus_tag="PC621_001662"
     CDS             56..1222
                     /locus_tag="PC621_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF4108823.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            1244..2890
                     /locus_tag="PC621_001663"
     CDS             1244..2890
                     /locus_tag="PC621_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF4108824.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            2987..4360
                     /locus_tag="PC621_001664"
     CDS             2987..4360
                     /locus_tag="PC621_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4108825.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            4805..5758
                     /locus_tag="PC621_001665"
     CDS             4805..5758
                     /locus_tag="PC621_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF4108826.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            5796..6758
                     /locus_tag="PC621_001666"
     CDS             5796..6758
                     /locus_tag="PC621_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF4108827.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            6783..7769
                     /locus_tag="PC621_001667"
     CDS             6783..7769
                     /locus_tag="PC621_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF4108828.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            7782..8828
                     /locus_tag="PC621_001668"
     CDS             7782..8828
                     /locus_tag="PC621_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF4108829.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            8995..10725
                     /gene="lpdA"
                     /locus_tag="PC621_001669"
     CDS             8995..10725
                     /gene="lpdA"
                     /locus_tag="PC621_001669"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF4108830.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            11143..14091
                     /locus_tag="PC621_001670"
     CDS             11143..14091
                     /locus_tag="PC621_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4108831.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            14069..14533
                     /locus_tag="PC621_001671"
     CDS             14069..14533
                     /locus_tag="PC621_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4108832.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            14646..14948
                     /locus_tag="PC621_001672"
     CDS             14646..14948
                     /locus_tag="PC621_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108833.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            15010..16374
                     /locus_tag="PC621_001673"
     CDS             15010..16374
                     /locus_tag="PC621_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF4108834.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            16409..16882
                     /locus_tag="PC621_001674"
     CDS             16409..16882
                     /locus_tag="PC621_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108835.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGDLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLRFRNKKFENKNI"
     gene            17054..17704
                     /locus_tag="PC621_001675"
     CDS             17054..17704
                     /locus_tag="PC621_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF4108836.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            17878..18810
                     /locus_tag="PC621_001676"
     CDS             17878..18810
                     /locus_tag="PC621_001676"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF4108837.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            19084..19797
                     /gene="ispD"
                     /locus_tag="PC621_001677"
     CDS             19084..19797
                     /gene="ispD"
                     /locus_tag="PC621_001677"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF4108838.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            19794..20279
                     /locus_tag="PC621_001678"
     CDS             19794..20279
                     /locus_tag="PC621_001678"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF4108839.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    20374..20582
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20646..22361
                     /locus_tag="PC621_001679"
     CDS             20646..22361
                     /locus_tag="PC621_001679"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF4108840.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            22483..23928
                     /locus_tag="PC621_001680"
     CDS             22483..23928
                     /locus_tag="PC621_001680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF4108841.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            24368..25849
                     /locus_tag="PC621_001681"
     CDS             24368..25849
                     /locus_tag="PC621_001681"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF4108842.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            26070..26225
                     /locus_tag="PC621_001682"
     CDS             26070..26225
                     /locus_tag="PC621_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108843.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            26370..27767
                     /locus_tag="PC621_001683"
     CDS             26370..27767
                     /locus_tag="PC621_001683"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF4108844.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            27752..28165
                     /locus_tag="PC621_001684"
     CDS             27752..28165
                     /locus_tag="PC621_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF4108845.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            28272..28367
                     /locus_tag="PC621_001685"
                     /pseudo
     CDS             28272..28367
                     /locus_tag="PC621_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            28673..29503
                     /gene="thyA"
                     /locus_tag="PC621_001686"
     CDS             28673..29503
                     /gene="thyA"
                     /locus_tag="PC621_001686"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDF4108846.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            29516..30010
                     /locus_tag="PC621_001687"
     CDS             29516..30010
                     /locus_tag="PC621_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDF4108847.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            30037..31413
                     /locus_tag="PC621_001688"
     CDS             30037..31413
                     /locus_tag="PC621_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF4108848.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            31389..32489
                     /locus_tag="PC621_001689"
     CDS             31389..32489
                     /locus_tag="PC621_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF4108849.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            32532..32957
                     /locus_tag="PC621_001690"
     CDS             32532..32957
                     /locus_tag="PC621_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF4108850.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            32970..33707
                     /gene="rlmB"
                     /locus_tag="PC621_001691"
     CDS             32970..33707
                     /gene="rlmB"
                     /locus_tag="PC621_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF4108851.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            33710..34237
                     /locus_tag="PC621_001692"
     CDS             33710..34237
                     /locus_tag="PC621_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF4108852.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            34302..34949
                     /gene="sigH"
                     /locus_tag="PC621_001693"
     CDS             34302..34949
                     /gene="sigH"
                     /locus_tag="PC621_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF4108853.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            35023..>35414
                     /locus_tag="PC621_001694"
     CDS             35023..>35414
                     /locus_tag="PC621_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF4108854.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT        14460 a         3694 c         6722 g        10538 t
ORIGIN      
        1 ttattataag tgcaaaatat acctatttaa ataagataag aggggaggat tttatgtgta
       61 tataaaaggt gtaaatttag gaggttggtt agttttagaa aaatggatga cctcaagttt
      121 atttgaaggc acagaagctg aagatgaata ctatttacca agacagcttt caagagaggc
      181 atatgaatct agaattaaga ctcatcgtag tgaatacatc acagagagag attttgcaac
      241 gattaaatct atgggattta attcagttcg tataccagta ccatacttta tttttggaga
      301 ttgtgaacca ttcataggat gtgttaaaga acttgataaa gcatttgctt gggcagataa
      361 gtatgggctt agtatattga tagacttaca tacagttcca ggaagccaaa atggatttga
      421 taatggagga atatcaggta tatgtagctg gtcacaaaat ccagaatatg tagcgtttac
      481 attaaatgta ttagaaagac ttgcaaaacg ttatgggatg agacatgaat tgtatggaat
      541 ccaaatttta aatgagccta taacagaaag aatgtggaac ataatgaatg ttccaaatcg
      601 gtttaaggct gtagataagg agatggcaag ggggagtaaa cctaattcat tagagttttt
      661 acgtgatttt tatataaaag catataaggt aatgcgtcct tatatgagag aggaaaacgt
      721 tatagtgttt catgatgcat ttgaattaaa agcatggaaa gactttatga gagaggaaga
      781 atttaaaaat attgttctag atacacacca atatttgatg ttagctgaat cagatggatg
      841 tgaacaaagt attgatagct atctcaaata tattagagaa aattatgcta aagatatttt
      901 acaaatgcaa aaatattttc ctgttatatg tggggagtgg agcttattta attcatatgc
      961 ttgtggtata gatacaaatg gaggtcaatc acctctaaat gggattgaat caaatataga
     1021 taaattatca aaagacgata aaagagaatt atataggaag atagcaaaag cacagcttga
     1081 tgcatggaga aatggaagtg gtcactacta ttggaattat aagttattac tagatacagt
     1141 taatgaagaa ggttggatag gatgggatag ttgggaccta ggcaaatgtg tagctcaaga
     1201 atggtatcct atagaatatt aaactaaaat tggaggaaaa gatatgatta aaaaccctat
     1261 acttccaggt tttaatccag atccttgtat ttgtagaaag ggagatgatt attatcttgt
     1321 agtttcatct tttgaatggt ttccaggtat acctgtttat cactctaaag acttaaaaaa
     1381 ttgggagtta tatactcata ttttgacaga tgaaacaaaa atagatttga agaagcttcc
     1441 ttcatctaaa ggaatttggg caccttgttt gacttattgt gaagaagaag atttatttta
     1501 catagtttat ggaattatga actctatgaa tgcaagatat tttgatgttg ataattattt
     1561 aattacatca aaagatataa agggagagtg gagtgaacca gtatatatac actcatcagg
     1621 ttttgatgcg tctattcttc atgatgatga tggtaaaaag tggattgcat cattagactg
     1681 ggagactaga gaaggatatg aaaaacctgg tgttatatgt ttagtggaat actgtacaaa
     1741 aaagaaagaa atagtaggat atccaaagag aatatggtca gggggaactg atagaggttg
     1801 tattgaagca ccacatataa caaagcgtgg agattactat tatataatgt gtgcagaagg
     1861 tggaactgga tatggtcata gtgtaactat gggaagggct aaaaatatat ggagacctta
     1921 tgaaaaagat tcaatgaatc caattgtaac ttcaattcca ggcgattttt atgaaagaca
     1981 tgaccctgac catctaaagc ctaaatacta taatccagaa tcaaaacttc aaaaatctgg
     2041 tcatggaagc tatatagaaa caacatcagg tgaagtatat ttagttcatc taacatctag
     2101 accatttgta ccagagttaa ggtgtacttt aggtagagaa acagctattc aaaaaatgaa
     2161 atggacaaag gataattggc ttcgaatgga agatgaaagt aatttggcaa aagaatacgt
     2221 atcagaaagt aaactagaag aacatttagt ttcatctata ccaagttttg atgactttga
     2281 ttcaaatgta ttaggtttac agtactatgc tcctaggatt tcaccattat catttgcaga
     2341 tgttaagtct agaccaggat atgtaaggat tagaggtcaa gaatcaagaa catcattaaa
     2401 taaagtaagc atattggcaa gaaaattaac ttcagtatat gcaagaataa ccactaagat
     2461 ggagttttac ccagaggtac atcagcatag tgcgggtttg ataatgtatt atgataatat
     2521 gaattatata aatttaagaa agtattatag tgaaacattg ggtcaaagtg cactatcaat
     2581 aatacatctt gaaaatggag agaagacgga atttttaaat actaggatac caataaaaga
     2641 tataccaata tatcttcgtc tgtatataca aggtcgtaag tcatattttg aatggagtta
     2701 tgatgaaaaa aactaccaaa gaataggaaa ggtatttgat acaactaagt tttcagatga
     2761 gtactgtaaa tatggagaat ttacaggtac gtttatagga ttaacttgtg cagatcgtgt
     2821 aaagcataaa cactatgctg attttgattt ttttgaatat atagcagatg aatcaaaaga
     2881 tgtcgattaa taaaatcatt actaaaattt tcagagcaaa ggtaaatctt atgctctgaa
     2941 ataagaaaca ttagaaggca aatcttacaa aaaggagtat ggtattatga atacagaagt
     3001 taatgttaaa gcaactacga ttagatatga aaagatttca ttttttgaaa gaatttctta
     3061 tggttgtggg gatttggggt gcaatatcat atactcagct atgtcagcat ttttgctgtt
     3121 ttattatact aattatgcag atgttagtgc agcagctgta ggtagtatta tgcttgtatc
     3181 taggatactg gatgggttta gtgacttaac aatgggaatt atagtggata gaactaaatc
     3241 caaatacggt aaggctaggc cttggatttt gagaatggcg attccttttg ctatagcagc
     3301 agtattatta ttttctgttc catcaaatct tggtatcact tctaaattaa tttatatttt
     3361 tattacatat aacttagttt ctactgttat ttatacagca atcaatgtac cctatgcaac
     3421 tctaaattca cttataactc aaaaccagta tgaaagagga gtattaagta tatttagaat
     3481 gatattagcc acttgtggta ccttactaat aaatggactt acactacctc tagttgaata
     3541 ctttggaaat aatttatcag catggactaa aacattcttt gtatttggaa tagcatctat
     3601 aatggtattt tttataacat ttactggaac caaggaaaga gtaaaagcag ttaagcaaga
     3661 taggaatgaa gtaataccat ttaaaatagg tattaaatct ttatttagaa ataagtactg
     3721 gatacaaata acattatgtt tagtttgcat atttattgta tttgcgataa atggtggttc
     3781 atccgtatat tatgcaaagt ttattttggg agatgaaaaa ttatttggtc ctataaatat
     3841 ggtttcaaat atatcccaaa ttatagctat gtttatggtt gctcctttta taaaaaaatt
     3901 tggaaaaaga aatgtattaa tagtaggttc aataatatta atatcgtcaa atgttatgtt
     3961 tataatagtt ggacaaaact atataggagt aatatttgca agtgctataa agggaatagg
     4021 aagtgctgga atagcatcaa caatgtttgc tattgtttca gatactattg aatatggaga
     4081 atggaagact ggatatagaa ctgaggggct tataaatagt gcatctagtt ttgggtttaa
     4141 agtgggaaat ggattaggtt cagcaatatt aggtgcagta ttatcaattg gtggttatgt
     4201 aggaacatca gctactcaaa gtgatttagc tatagtatca ataaaagtat gttttatata
     4261 cctacctata tttataacaa tattacaaat tatcataatg tttttttata agcttgataa
     4321 agaatatagt actatactta atgaattaaa tactaaataa tgaaaaagtt tcttataaag
     4381 tcttaagttt aattaaggtc tattgtaaat gttccctata aaaactatta tatatttatg
     4441 attattgaag aatttttaga gaagcgaata ataaaaatat aagaagactt taaatgatga
     4501 aatggttggt cttttagaaa tgatttaata agttattttt atttaaagca ttataagtgg
     4561 acaattaatt tttaatttat ggagatgata tttaacaatt agaagtaata tataatatgc
     4621 gctaagaagc aatttttcaa ctatttatga aggtggtggt acaagaaaag tatcaccttt
     4681 tttttgttat acaaaaaaac agttgacttt ttatatgaac cccttctata atgttactga
     4741 aattaagtaa aatgattgaa caaatgtaaa gataattgaa aagcttgatt ttggaggaaa
     4801 tcaaatgaaa aaaattggag acttaagatt aatgatgaag tgttgttcac tttattacga
     4861 agataatttg aatcaacagg aaatagcaaa tcaattggga atatcaagac caacaatatc
     4921 aagaatatta aaagaagctt ttgaacaagg aatagtaaaa atacagatag ttgatgtttt
     4981 gaaaaatgat taccaaaaaa tagagagaag tttggagaga aaatataaat taaaagaagt
     5041 aatagtagta gatgataaac aagatgcact aacacaaaaa caagaactag caagagctgt
     5101 atctgaatat ttaactagag tagtaaagga aaatgatata ataggtgttt caataggaac
     5161 aacactaaaa gaaataccaa gatatgtgga aaaaagtaac tgtaaaaatg ttacatttat
     5221 acctctatta ggagggattg gagacaatga aatagatatt catgcaaatc aaatagcagt
     5281 aagtctagcg agggcttttg gaggtgattt taaattatta catgctccag ctgttatgtc
     5341 tgacttgtct acaaaagaga aactttgcaa agacgaaaaa attaaagaag tactagattt
     5401 aatagataaa acaactatag caattgtggg tataggaaat cctatgagtt taaactcaac
     5461 aataatggct tctggataca tgaatgaagc agatatagaa gatttaaaaa aatacaactc
     5521 aataggtgca atttgtttac aagcctttga taaagaagga aaaacttcta tacttgaatt
     5581 taatcaaaga gtattaggag tgaaactgga agatttaaaa aagataaaaa gaacaatagg
     5641 tgtcgcttca ggagatgaaa aaatagaggc aataaaggca tcattaaaag cgaaatttat
     5701 aaatagttta gcaattaatc acagtttagc acttaaatta ttagaagatt gtgactaata
     5761 gaaagataga taaaagttaa caggagggaa tgtatatgtc aatatcaaaa gaaacattgc
     5821 ttgaaatgta taagagaatg aatcaggcta gaaaatttga agagaaagtt agttggtttt
     5881 ttgcaagagg tatggttcat ggaacaactc acttatctgt aggtcaagaa gcgtcaagtg
     5941 tggcggctgt aatggcttta gaaaaaggag atttagtatc attaactcat agaggacata
     6001 gtcaatttat aggaatggga atagacctaa ataaaatgat ggctgaactt atgggtaaag
     6061 aaactggatt ttgtaaggga aaaggaggct caatgcatat agcagatatt gagtcaggta
     6121 acttaggagc aaatggagtt gtaggtggag gtcttacaat agctccagga gctgcactta
     6181 cacaacagta taaaaaaaca ggcaaaatag ttttatgtag ttttggtgat ggagcatcaa
     6241 atgaaggtac ttttcatgaa ggaataaatt tatcttcaat atggaaattg ccaataatat
     6301 tttactgtga gaataattta tacggtatgt caacaagcat aaaacgtcat atgaatatag
     6361 aaagtattgc cactagagca gcttcttatg gaatagaagg aatatcaata gatggatata
     6421 acccaataga agtatacgaa acagtacaaa aagctgctga gaagtgtaga agaggagaag
     6481 gacctgtatt aattgaaagt agaacttata gatggttagg tcattcaaaa tcagatgcaa
     6541 atgtatatag aacaaaagaa gaaatagaat catggaaagc aaaagaccct attgaatttt
     6601 taaaaaatta tcttatagaa aataatctat ctaatgaaga tgaattagat aagattcaag
     6661 aatttgcaaa gcaatcaata gaagatgcag tagagtttgc tcaaaatagc ccaaatccaa
     6721 aaatagagtc tttattagag gatgtatatg cagactaaga atggtaaagg aggaaaataa
     6781 aaatgagtac aagggaatta acatatgcac aagcaataaa ggaagcaatg tcagaagaaa
     6841 tgagaagaga tgaaaatgta atatttatgg gagaagatat aggaatatat ggaggggcat
     6901 ttggtgtatc agttggtatg atagatgaat ttggaccaga aagagtaagg gatactccta
     6961 tatcagaagc agctatagca ggagcggcag caggtgcagc agcaactggt ttaagaccta
     7021 ttatggaagt aatgtttatg gactttgtaa caatttctat ggatgcaatt gtaaatcaag
     7081 cggcaaaaat gagatatatg tttggaggaa aagcacaagt tccaatggtt gttagatgtc
     7141 caggtggttc tggaactggt tcagcagagc aacattcaca aagtttagaa gcttggtttt
     7201 gtcatgtacc aggagtaaaa gtagttgcac cttctacacc agcagatgct aagggattgt
     7261 tgaaagcagc tataagagat aataatccag tcatatttgt tgagaataaa ttgttataca
     7321 gaaaaaaagg ttttgtgcca gaagatgatt atgtaattga aattggaaag gcagatatta
     7381 aaagagaggg tactgatgtt acagtaatta cttatggaag aatgttacaa agtgttgaag
     7441 aagcagcaga gactctatca aaagaaaata taaatgttga aattatagat ttaagaactt
     7501 tatatccatt agataaagag actatagtaa agagtgtatg taaaactggg agagttttaa
     7561 tttgccatga agcagctaag actggtgggc taggtggaga aatatcagcc ctgataacag
     7621 aaagtgaatc atttgattat ttagatgctc cagtaaaaag aatttgtgga aaagatgtgc
     7681 caataccata taatccagag ttagaaaaag cagttgtccc aagagttgat gaaatagaag
     7741 aagcaataaa atctttaatt gttagataag gagggataga tatggtggca aataaaataa
     7801 aagccacacc agcagctaga agtcaggcaa gaaaagacaa tataaaatta gataggctga
     7861 ttggaagtgg agaaaatgga aggattcatt tagtagatgt gttaaattat ttaaaagata
     7921 ataaagcaaa tactactcct ttagctagaa gaatagcaga agatttaaat atagatttag
     7981 aaactattgt aggtactgga tatagtggaa agataagaaa gtgtgatgtg gaaaaattaa
     8041 ctgcaaaaga aactattgta tcaaataata catctaaata tagtgagaaa aaagagctga
     8101 aaattgaaaa tgaaaattca agcatgttta acacagtaga aggaatattt gaaaagccaa
     8161 atccaatgag agctactgta gcaaaacgaa tgtcagaaag ttattttagt gctcctgtat
     8221 ttacattcaa tatagaagta gatgctacag aattgaaggt acttagagca aagttaatcg
     8281 acacagtaaa agaatcaact ggagtaaaat taactatgac agatttaata gtaatggcag
     8341 tatctaaaat acttccaaat catcaagcct taaattcagc atggacagat gagggtatat
     8401 ttagatataa agatgtaaat atagctattg cagttggttt agatgaaggc ttatatgttc
     8461 cggtagtaaa aaatgcaaac aaaaagtctt taaaagaaat agcaaaagaa agcaaagaac
     8521 ttgcggaaaa agtaaagaca gggaaactta tgccagcaga ccaagaaggt aatacattta
     8581 caataagtaa tgttggaatg tatggtataa ctacatttac acctataata aatatgcctt
     8641 caagtgcaat cttaggggta ggtgctacac aagataagtt tgttccagta aatggagagg
     8701 ctaaaataaa acctataatg aacttatcat taacatcaga ccatagagta atagatggaa
     8761 cagtagctgc aaaattctta aaagatttaa aagaattgtt agaaaatcct ttatcaatgc
     8821 ttgtataggt tttgatattt cgttactgat ataaatagtt tgaatagatt atattattat
     8881 aattgtttca gtaaatttaa agcttttata tattaagaat tatttttaac agtaactata
     8941 tgtgaatatg atttgcttaa ttaaaatata tgaaaataaa taggaggaga gcctatgtca
     9001 gtagaagtaa taatgccaaa agcaggagta gccatggaag aaggtactat agtatcttgg
     9061 ctaaagcaag aaggcgaaga agtaaagatt ggagaaccta tactagaaat aacaacagat
     9121 aaagttaata tggagataga gtcagaagga gaaggtacac ttgctgtaat catacataaa
     9181 gaggaaggtg aagtattacc agtatttaca gtaataggtg taatagcaga aaaaggggaa
     9241 aatcaagagg aagtaaaagc taaatattta tctggaaatg tttcaaaaga agatatagtt
     9301 gaagagaatc aaaatataga agttaaagaa gaaaaaataa acaaaaaaga gtgtaatcat
     9361 gattatgatg tagtggtaat aggaggagga cctgggggat atttatcagc attaaaagca
     9421 gctcttttag gtggaagagt tgctttagtt gaagaaaata tactaggagg tacttgctta
     9481 aatagaggtt gtatccctac aaagacttat ataaaaacag cagaaatatt agaagaaatt
     9541 gaccaattat caaagagagg tgtaaaagta actgtagata aagaacagga cattaagaag
     9601 gccataaaat ataaaaatag agtagttaaa aaacttacag ctggtgttgg cggattatta
     9661 aaaagtagag atgtagatgt gtttaatctt aaagctagtg ttaaagaaga gcataaagta
     9721 attttatcag atggaaaagt tttagataca gaaaatataa taattgctac aggttccaaa
     9781 gtaaggatat tacctataaa aggaatagaa tcaaatttaa taataactag tacagaagca
     9841 ttagatttgg aaacagtgcc agaagaatta gtaataattg gtggtggagt gattggttgt
     9901 gaatttgcag agatattcaa ttcaagaggt tctaaagtaa ctatagtaga aatggaagat
     9961 agagtaatcc caagaatgga taaagagtta agcgaatctt taaaatattc tcttagtaaa
    10021 aaaggtataa atgtattaac taaaaagaaa gtttctgaat ttaaagaaga aggaaataac
    10081 atattagtgt gcattgaagg tgaagtgcca ataaaagcag atttatgtct atatgctata
    10141 ggaagagaag ctaatctttc tggcatagag gacttagata ttaaaataga taaaggttca
    10201 atagttgtaa atagtaaaat ggaaacaagt ataccaagca tatatgcagt aggtgatgtt
    10261 actggaggtg taatgttagc tcatgctgct tttaaaatgg gagaggtagc agcttcaaat
    10321 gctctaggtg tgaataaaga agttgactta ggtgcattac ctagttgtgt ttatacaatt
    10381 ccagaagtag catcagttgg tattacagaa gaggatgcta gaaaaaaata taatgtaaaa
    10441 gttggtaagt ttaattttgc tggaaatgga agagctttag cttctggtca agaacaagga
    10501 tatgtgaaag tagtagcaga tgctaaatat ggagaaattt taggtataca tatgtttgga
    10561 tgtggtgtag cagaacttat aaatcatgca gcatcattta aagcattaga gatacctact
    10621 gatgaagcaa gtgagttgat atttggtcat ccatgtactt cagaagcttt aatggaagct
    10681 ttggcagatg taaatggaga atgtttacac ttacctaaaa aataaatcta aacataaatt
    10741 attaatttaa gtctattatt aatattaatt tatatcataa aattttaaaa cacttactta
    10801 gccatacccc ttattatgca aataatttat ttgtataata aggggtattt tttatggaaa
    10861 aatatagaat atagaatcct tgtagttttt gttgtacaaa aaagatagta atataaaata
    10921 acattatcat tttagaatga tttcaattgt aattaggaaa agtgattttt ttgtttttaa
    10981 aaaaagtatt atttgtaaaa tataaaaaaa aatttcttaa tatttgaata aaagaatagc
    11041 ttaatttaaa tgttattact ttataaaaaa ataataagta caaaaaaaac aatatatggc
    11101 atataagttg ctatataaat aataaggagg gaacaattat aaatgaggca aagaataaaa
    11161 aacttaattg aaaatgaaga taagaaaaat cctttaacag atgaagcgat ttcaagtagg
    11221 ttaaatattc gaagagaaga tgttaccttt ttgagaaatg agttaaaaat agaagattca
    11281 agacaaagaa gaaaagttgt tttaattaaa gctataaaag atattttaaa agaagaaaaa
    11341 actataaata aaaatgatat aacaagaaga ttaaacagca ttgggtttac agtatcaaga
    11401 tttacagtca tacaatactt aaaagagatt caagattctg gtgacctaaa acttgataaa
    11461 gagagtgatg ataaaaaaga agaaataatc aataatagag aaaataaaga tatagcattt
    11521 gataaactca taggtagtag tggtagctta agtacaatta taaaactagc caaagcagca
    11581 attctttatc cacctcatgg tcttcatact attattatgg ggccaactgg tgttggaaag
    11641 agtgagttag cagagtgtat gtataaattt gcagtagaat catccagatt ccctaagaat
    11701 gcaccattta tagtttttaa tgcagcagat tattcagaga atccacagct tttgctatca
    11761 cagttatttg gacacgttaa aggtgctttt acaggagctg atgagaacaa agaaggatta
    11821 gtatcaaagg cagatggagg aatattgttt atagatgaaa tacatagatt acctcatgaa
    11881 ggtcaagaaa tactatttca actgattgat aaaggtatgg tcagaaagtt aggagaaaca
    11941 agactaacac ataaaataga tgtaatgatt atttcagcaa cttctgagcc aatagattct
    12001 catttactta atacatttaa aagacgtata cctgtaacaa ttgaaatacc agaattagtt
    12061 gcaagaccat taaatgagag gtttgacatt ataaataatt tcttcttaat agaagctcaa
    12121 agaatgggag caaacataca tataaagtca gatgtattaa aagctcttat gctttacgat
    12181 tgtataggta atgtaggtca acttagaagt gatattcaag tagcttgtgc taggggacta
    12241 ttaaaccaac ttacaaacaa actaaaagaa gtaaatataa gcatatcaga tgtacctgga
    12301 tatgtaaaac aaggtcttat aaaaatcaga aattgtagag gtaaaataga aaattatgta
    12361 gatggggatt taattataga ttcaaatctt caaggtgaga tagaaaagaa aaatgaagat
    12421 atatatactt ttccagatga aatatacaaa cttactgaaa gaagacatat tgatttattg
    12481 gaacaaggtt tagattttga tgttataaat agaatcattg gaggagaatt agaaagttgc
    12541 attcaaaaat acataaaaca agttaaaaaa gtttctatta gtaacaatct tccagatata
    12601 agtccaattg ttggaaaata tatcgtagag ctaacttatg aagtaatcaa aatagcatca
    12661 caatacctaa gttcactaga accgaatcta catttatgtt tagcagttca cctaaaagca
    12721 gcttttgaga gattaaaaga aggaaaagta atatcaaatg ctcatttgaa agaaataata
    12781 ggaaattatc cactagaata ttctgtagca gaaaaggtag ctcaatattt aaaggaaaga
    12841 tataatataa acttaccaaa agaagaaata ggtataatag caatatattt taaaatgagt
    12901 agtagaagag atatgtctga acaaaaaaag ataggtgtac ttcttatggc tcacggaaga
    12961 gtagcatctt ctgtatgtga ggttacaaat aaactattag gaattaatca cgcaaggtat
    13021 ttagatatgc catttgagaa gaagccggaa gaaatgattg aagaagctac tacaataata
    13081 aaagatattg atgaaggtag aggtgtgatt atattagtag atatgggttc actttcatat
    13141 tttggagata ttatttctgg aaagactgga attaatataa ggacaatatc tagatttgat
    13201 acattgcttt ctatagaggt tatacgaaga gcagttttac cagatttaac aatagacgag
    13261 atagttgatg aagttaaagg tatagagcca caagtaaaag aaaaggttag taaatgtaac
    13321 aatacaaaag tagattttag aaaacctata attttaacat tatgtattac tggattaggg
    13381 ggagcttgta agataaaaaa aatcctagag gaaaatatta aaagtataac tgaaaaggtt
    13441 gatatagaac ctataggtat gattgaatat ccaagtcttg atttagagat aaaacaaata
    13501 agcaatgata aaaatgtaat cgccatagtt ggaacaattg accctcagat agcaaatatc
    13561 ccttttttta gtctagtaga cataaatgat aaagcaaagt taaatcaaat aaataaccta
    13621 attgatacaa atcaaattgg tgaaacaaat aaaaaatatg aattatcaga tttaatacat
    13681 aaagaagata tatttgtgga tttacaggtt aaatcaaaag aagaagttct aaaatatatg
    13741 agtaaaaaac ttagagagaa aggttatgtg acaaaaaatt tctataaaaa agtagtggaa
    13801 agagataact tatttccaac tgaattatca gatgaaatag caatacctca cacagatgga
    13861 atagatgtaa ttaggccagc tataagtata gccatcttaa agaagcctat tgtgtggagt
    13921 aaaagtaaag taaaaataat acttttatta gctgtagacc aaaagtgttt taatccatta
    13981 agcactctac tctcatttat agaaacagat gaatttaaga agataaaaaa cattaaagat
    14041 agagatttta ttagggagat gatattagat ggagtcaatg aaaataatta gtagcaattt
    14101 aatttttaaa aatattgaag tgtctaacaa tgaagatgca ttaaagtttt tgggacaacg
    14161 tttgtttgac gaacagtatg taaaagaaag ctacatacaa gctgttgtag caagagaaaa
    14221 aaagtatgct acaggattac caacagaaat atacggagtg gcaataccac atacagatat
    14281 agtacatgta aatgagccag gaatagcaat tggaatactg aataaaccag taaaatttat
    14341 aatgatgggt acagacgata ctgaaattga tgtaaaagtt gtatttatgt tagcagtgaa
    14401 agagccacaa gaacaattgc aactattaga aaaacttatg actatatttc aagataagaa
    14461 tatactaaat aatattgttg acttaagtga agaaagtgtg agtgatttat taaactctaa
    14521 attgaaaaat taaacatgta aagtatatta aaaatgtaaa attaaacatg caaagtatgt
    14581 taaaaaatat aaaaagatta ttaaagattt ctaactatag atatctttaa aataaaataa
    14641 tttcaatggg ggtgactaat gtgactaaaa agatattagt tgcatgtgga acaggagtat
    14701 gtacttcaac aattgctata aacaaattaa agaaagcact tcaagacatt ggtaaattgg
    14761 atatggttaa tattacacaa tgtaaagtag tagaagttgc atcaaaagca cctgattatg
    14821 atttaataat ttgtactaca caagtatcta gtagtataaa aacaccagtg ataaatggat
    14881 tacctttttt aactggtgtc ggaatggata aattaatcaa tgatgtatta gaagagttag
    14941 aattataatt aaaaaaatta aggaacatgg tctctaaaat cataagtttt agagacttaa
    15001 aattaaataa tggagggtct ttatatggag atattaaatt tcattgttgg tctaggtgca
    15061 caagtaatga tgccaataat aatatgtatt tttggtttga tattaggtac aaaactagga
    15121 aaatctttaa gagctggttt aactgtggga gttgggttta taggtcttaa cactattata
    15181 gctttgctaa cagataattt aggtccagct acacaacaaa tggttaagaa tttagggcta
    15241 agtttatcaa taatagatgt aggatggcca gcagcatcag ctatagcttt tgcatcaaca
    15301 gtaggggctt taattattcc aataggtctt gtggtaaata tagttatgct aattactaat
    15361 actactcaaa ctgttgatgt agacatatgg gattactggc attttgcttt tacaggagct
    15421 ttagtggcag gggctactca aagtgtaatg tggggagtat ttgcagcagt agcaaatatg
    15481 gtaattgtat tggtaatggc agatttaact gctccaggta tagaggaata tttaggtatg
    15541 ccaggtatat ctcttcctca tggatttaca caggcatttg ttccaattgc tatagtagta
    15601 aataaattat tagacctaat accaggtata aataagattg agataaatgc tgatacattg
    15661 cagaaaaaat ttggattatt tggagaacct ttaataatgg ggtctgtaat aggtgtaata
    15721 attggtattg ctgcgaaata tgatataaaa ggaatattac aattaggtgt aactatggga
    15781 gctgtattaa tattaattcc aaagatggca gcattattaa tggaaggatt attaccagta
    15841 tcagaagctg cacaggaatt tattgagaag agatttaaaa acagaggcaa gatttatata
    15901 ggcttagatt cagcagtagg aataggtcat cctgttacat tatcagttgc actagtgttg
    15961 gttcctttga ctatacttat agcagctata ttgccaggaa ataaagtttt accatttgca
    16021 gatttagcag taattccatt tgcacttgta ttaatcgtac caattacaaa gggaaatgtg
    16081 tttagaacat taataatagg aataattata attacttctg gattattaat cgcaactaac
    16141 ttagctccat tatttaccca aatggcttta aatgctagct ttaaaatgcc tgaaggagca
    16201 actatgattt caagtatttg tgatggtgcg aatccattaa gttgggtatt tgtaaaagtt
    16261 atgaactata aagttattgg aggagtagtt tttggagtta tagctttagg aatggctatt
    16321 tacaatagga atagaataat aaatgaaaat aaaaagctat ctatagaaga ataaggttaa
    16381 aacaatttta ttaaggagtg tataggttat gaaaaaatat aaatatgatt ctaaaaggta
    16441 cttactcagt gttacaatac caggattttt tatgatttta atattgattt atgcactgtt
    16501 taataactat ataaatttta atttgaatat atatagtttg ttaataattg tctgtactta
    16561 tggtatattc aatactttta tatcatcaag taatcctaaa aaaattatta ttgacagcaa
    16621 atgtatacat tttacctcat ttatgactac acataagtat gaaattaaag atatagaaaa
    16681 gataagaata agagaatttt ataataaaaa aatttatcta aaaattaatg atggagacct
    16741 ttttaaaggt aggtattgga taagaacaaa tatgtttaat aatagtatag cattatatag
    16801 ttatttcatt gagttggaag agtgcttata tccagattca ctaagattta gaaataaaaa
    16861 atttgagaat aagaatatat aaaaataaaa aattgaattg aagcctaact taatcttttt
    16921 tgataagata taagtttgaa ctttatacta aatttttaga attttcaatc aagacaataa
    16981 cataatagct aagcaattag aacacaacta agatgtataa ggcatgtata tgatgaatgg
    17041 gaggatttaa ttaatgttat tagagaaatt aagaaaagaa gtattacaag catctttaga
    17101 ccttttaaac tataatttag tgacattaac aggagggaat gtaagtggga gagatgaaca
    17161 aactggctat atagcaatca ccccaagtgg aatggattat agaaatctta caccaagtga
    17221 tattgttata gtagatgtag atggaaatat tatagatgga aaatggaaag catcagtgga
    17281 tttatcagac cacttgtata tatataagca tagagaagac ataaatagta taattcatac
    17341 acattctaca tactcaagtt gttttgctat tttaaatgaa cctatagaat gcgcaagtac
    17401 aactcttgca aatgaagtag ggggttcagt accagttgca aaattttcac caccaacatt
    17461 taaaaagatg ggaaaatgtg ttattgaagc aattggtgac aaaagagcat gtcttttagc
    17521 aaatcatggc gtaattgcag ttggtccatc tgtaggacat gctcttacag ccgctgtaat
    17581 gttagaagat tcagcaaaag tatattactt agcaaaatca attggcacac cagtgctact
    17641 gcctgatgaa gaaattcaaa gagcgagaga tgtatttttt aatgtctatg gtcaggataa
    17701 ataaattaga taaaatttta aaagtaatat gaaaatatag atattcaatg atattaagga
    17761 gatttataat gatataataa gtttaatagt taagaagtaa aatatgaaat gtaatagtat
    17821 ataaattcat ttaagactaa aaataataag ataaaataaa aaggagtctg tatttctatg
    17881 tattacttat taaacaaaag cacaaatcct tactttaatt tggcattaga agaatatcta
    17941 tttttaaatg ataaatataa tgatgatatt ataatcatat ggagaaatga agactccatt
    18001 tttataggta aaaatcaaaa tccatatcag gaagtacatc atgatgttat tgaaaaggga
    18061 gaaattccta ttttaagaag aatatctgga gggggcactg tttatcatga tttaggtaat
    18121 atcaatatgt cattcataca aaaagatagg caacttcatg agatagattt tttggagcat
    18181 actaagttta tgcaaaagat gctttcaacc ttaggactag atgtatctat aactgaaaga
    18241 aaagatttat ttttaaatgg gaaaaaaatt tcaggctctg cacagtctat aaaaagaaaa
    18301 aattctcttt atcatggaac tcttctttac gattcagatt taaataaatt aactaaatat
    18361 ttaaattcaa ataaagcaac tgaatcaaat gcaactaaat ctgtttcaag taaagttaca
    18421 aatattaagt ttttactcga aaaagatata aattactttt tagattattg tgttgaatat
    18481 ttaaaatcta atataaagaa tataaaagaa cttgaattgg atgaagaaga tattaaaagt
    18541 gtatacaata taggagaaga taaatataga aagatagagt ggacatatgg taaaacgccg
    18601 aaatttcaag tagtgctacc atttaataat gaaagtaaaa taaatataca tgttaacaga
    18661 tggaaagtat caaaattttt tatttcttat gatgataatg taattaatat ggataagttg
    18721 ttagatttga atttctttaa agaagaaata aaagagagta ttttagaaaa ttatccacat
    18781 tacatagatt taatcaattt aatattttaa tataaaacct ctaatttgat aaaattgtct
    18841 tatatcaaac tagaggtttt taatattctt gcatattaac aaccattaca aaattgtaaa
    18901 attaaaaaca acactattga aaaatcaata tatattgtaa taaatatatg tgtttttcat
    18961 tagcaaatag acattaacta tttatacaac tgaaaattta atatataatt aatcagatgg
    19021 ttaaaataaa aatattaaaa gttttaataa atgatggtta aaaataaaat acgaggtgat
    19081 accatgtaca gtgttataat tgttgcagcg ggaagcggaa ggagaatgaa cttagatata
    19141 aacaaacagt tcataaaatt aagagaaaaa gaaattattg ctcatacaat tcaagttttt
    19201 tatgaaaata taaatataga tgaaatagtg gtatgtataa aaaaagaaga agaggatttt
    19261 tttaaagaaa atataataaa taagtataac tttaaaaata taaaaatagc atatggcgga
    19321 aaagaaagac aggactcaat atataatggt ttaaaaaaat tagatgaaaa ttgtgatata
    19381 gtacttatcc atgatggagc aagaccattt gtcgaccata gaataatcaa cgaatctata
    19441 aaggtagcta aagaaaaaaa ggctgtggtt gtaggtgttc cagtaagtga tactataaag
    19501 atagtgtcag atggaacagt acaagaaact ccagaaagaa atttgttgtg ggcagcacaa
    19561 acccctcaaa cctttgaata taatctcata atagatgctt atgagcaagc atataagaac
    19621 aattattatg gtactgatga ttcaatgttg gtagaaaata taggtcaaag tgtcactatg
    19681 gtaatgggtt cttatgaaaa cataaaaata actagtccag aagatttaaa tatagcagaa
    19741 caaatattaa atatggaaaa aagagatgaa gttaacagta gaaggagaat tatatgagaa
    19801 taggattagg atatgatgta cacaaattaa cagagaatag aaaacttata atcggtggtg
    19861 tagagatacc acacgataaa ggtttgttgg gtcattctga tgctgatgta ttgatacatg
    19921 caataatgga ttctatttta ggtgctctag cattaggaga tattgggaag catttcccag
    19981 atacagatga agaatataag ggagcagata gtatgaaatt attagaacat gtgtataatc
    20041 taataacaag taaaggatat aaaattggta atatagatag tactataatt gctcaaagcc
    20101 ctaagatggc tccatatatt gagagtatga gaagtaatat atcaaaagtt ttaaacacag
    20161 acattgataa tataaacata aaggcaacaa cagaagaagg tcttggtttt acaggagcaa
    20221 aacagggaat agcttcacaa agtatttgtt tattattgtt gacaagtcag aataattaat
    20281 atattattag taataagttt aatggatatc aagtaatagg cctatataga atatctataa
    20341 tgtatgaatt taagtgaaat taatataaat ataaaattag ctatgataaa gaaatagtaa
    20401 aatgatgaaa tcttcacaga gagaaaacgg tggtgagagt tttcaagagg aagtctttga
    20461 acctgtcttt tagcttaaag gtataaaaat ctttacggta ggaaccgtta tattcccaaa
    20521 agagagctat tcatatagct aactagagtg gtaccgcgga aattaaacct ttcgtctcta
    20581 tacttgtata gagatgagag gttttttata ttttcaggaa gttaatattc aggaggaatc
    20641 tattaatgaa aatgtcaaaa atgtttatgc cgacattaaa agaaatccca gcagatgctg
    20701 agattacaag ccatcaatta atggttaggt caggcatgat aaaaaaaatg acttcaggtg
    20761 tttataacca attaccaatg gggttaagag tgttcaaaaa aattgaacaa ataattagag
    20821 aagaattaaa taaaaaagat tgtcaagaaa tactttgtgc agccttactt ccttctgaat
    20881 tatggaaaga gtctggaagg tggactgcta tgggtgaaga aatgtttaga ctaaaagaca
    20941 gaacagaaag agagtactgt cttgggccaa cacatgaaga agcattcact gatataataa
    21001 gacaagaaat tacttcatat aaacaattgc cactgaactt gtaccaaata caagtaaaat
    21061 atagagacga gagaagacca agatttggtg ttatgagaac taagactttt acaatgaagg
    21121 atgcatatag ttttgatgta gatgataaag gtttagataa gtcatatcaa gatatgtttg
    21181 atgcatatgt aagtatattt gatagatgtg gattggagaa tagccctgta caggcagatt
    21241 caggagctat aggaggttct acatcagccg aatttatggt taagtcagaa gttggtgaag
    21301 atgaagttgt attttgtagt ggatgtgatt atgcagccaa tgtagaaaga gcagaatctt
    21361 gtaatctagc atcacaaaaa gaagaaatga aagaattaga agaggttcat acaccaggag
    21421 cagctacaat aaaagagtta gaagaatttt taaaaacttc tccagataag tttgctaaaa
    21481 ctttagtata tgaggcagat ggaaaaacag ttgtagtcgt tgtaagagga gatagagaag
    21541 ttaatgaaat caaagtatct aatgctatag gttctgttat agaatttgct cttgctacag
    21601 atgatgtagt gagaaaagta actaatgcag aagtaggatt tgcaggtcca ataggtataa
    21661 atgctgacta tgtatttata gataaagaaa ttgttgaaca aagaaacata gttgttgggg
    21721 ctaataaaac tgagtaccat ataaaaaatg ctaactatgg aagagatttt gaaggaatag
    21781 ttggagattt tagaaatgtt caagaaggcg accagtgtat agtatgtggt aagccacttg
    21841 aaatagctag aggtgttgaa gtaggacata tatttaaact aggaactaag tactcagaat
    21901 ctatgaatgc aaacttcata gacaaagatg gaaaatcaaa gcctatagta atgggatgtt
    21961 atggaatagg tgttgaaaga acagcagcag ctataattga acaacataat gatgagaaag
    22021 gtataatatg gcctttgtca gtagctccat accatgttgt gatagttcca gcaaatatga
    22081 aaaatgaaga acaaatatca atagctgaaa atatatataa tgatttgcaa gctatgggtg
    22141 tagaagtttt attagatgac agagatgaaa gaataggtgt caaattcaat gattcagaat
    22201 taataggaat acctatgagg attacagtag gtaaaaatat taatgaaggt aaagtagaat
    22261 ttaaacttag acataaagaa gataaagaga taattgacat agaagaaata aatgaaaaag
    22321 taaaggcaga gtttatcagg aataatgtta ggttaggata ataacttaaa taagatattg
    22381 tatagataat caaagttgta ctttatagac accattttat ctatatataa ggatataaaa
    22441 tggtgttttt ttataaaaca aataaatgag gaggtaattg aaatggcaaa aaatgaaaaa
    22501 caatttgttg aagaaataac aaagatggaa gatgattttc cacaatggta cacagatgta
    22561 ataactaaga ctgacttagt tgattatgca ccagtaaaag gttttatggt tgtaaagcca
    22621 tatggatatg cattatggga aaagatgcaa gaatttatgg ataaaaaatt taaagaaact
    22681 ggacataaaa actgttattt tccattatta ataccagaga gtttattaaa caaagaagca
    22741 gaacacgttg aaggttttgc tccagaagtt gcatgggtaa cacatggagg aaataaaaaa
    22801 ttagaagaaa gattatgtgt aagacctact tcagagacta taatatgtac aatgtatgcg
    22861 aaatggttaa aatcatatag agaattacca tatctttata atcaatggtg ctcagttgtt
    22921 agatgggaaa aatcaactag acctttctta agaacatctg agtttttatg gcaagaaggt
    22981 catacattac atgaaacagc agaagaagca caagaagaaa caatacaaca attagaagta
    23041 tataaagcgt tatgtgaaga attacttgca atgccagttg tagctggtca aaaaagtgaa
    23101 agtgagaaat ttgcaggtgg tgaaagaaca tatactatag aagctatgat gcatgatggt
    23161 aaagcattac aatcagggac aagtcacttc ctaggtcaac actttactaa ggcttttgat
    23221 attacttttg cagatagaga aggaaattta gctaatccat accatacatc ttggggagca
    23281 tctacaagac ttataggtgg acttataatg actcatagtg ataacagagg tttagtatta
    23341 ccaccaagag tagctccaat tcaagttgtt atagttccta tagctgctaa aaaaggaaat
    23401 gtaatggaga ctgttgataa aatatatgct gatttaaaaa ctaagggagt agccgtagaa
    23461 gttgatgata gagataacta tacaactgga tggaaattta atgagtggga aatgaaaggt
    23521 gctccagtaa gagttgaaat tggaccaaaa gatatagaaa ataatcaagc tatggtattt
    23581 agaagagata ctttagaaaa ggattctatg cctttagaag gattagctga tgctatatgt
    23641 gatttatttg atgttataca caatgatatg tttgaaaaag caagaaaaca tagagaagac
    23701 aatacatcta tagttgaaaa tatggatgag tttagaaaag ctttagaaga aaagccagga
    23761 ttcataaaga ctatgtggtg tggagatgca gaatgtgaag ctaagattaa ggaagaaaca
    23821 ggagcaacta taagatgctt accttttgaa caagaaaact taggtcacaa gtgtgtgtac
    23881 tgtggaaaag aagcagatag tatggtagta atggcaaaag cttactaaaa tacatttaaa
    23941 taaaaactgg ggtagaaagt atttaatact ttatcccagt ttttttgttt tttatgagta
    24001 ataaaatgta tttgtaacta tgtcataaga taagtcatag atacttatat taaaaagaat
    24061 ataatagaga actattttta ataattacat attaatgaaa gcaaaatatt tttgttatat
    24121 agattttcaa taaagcaaac aataaatata aatgaatatt agtaaggagt gcttttaatt
    24181 tttagaaaat aaatgactta aaagaaagat ttatgaaatt aaaactagga ttttctgata
    24241 taataaacat gtgtattttt aaataaaaag gatattatta ttagatttga ttaaaaataa
    24301 acttgcaaaa caatgtacta gtttaatatt atagttatat tgaataaaat atcaggagga
    24361 agtaaaaatg aatgtaagag taaggtttgc accaagtcca acaggatttg tacatatagg
    24421 tagtttgaga actgctttat ataactattt atttgcaaaa aaaatgggtg gagaatatat
    24481 attaagagtg gaagatacag accaaagtag attagtagaa ggtgctatag agaatatgct
    24541 taatgctatg aaatgggctg gtgtcaacca tagtgaaggt gttatattag atgatagtgg
    24601 aaaagtagta caaaaaggag aatatggtcc atatattcaa tctcaaagat tagatatata
    24661 ccaagaatat ataaagcaac ttttagatag tggaaaagct tattattgtt tctgtacaaa
    24721 agaaagactg gatgaagtaa gagatgctca aagagcagca ggagaaacag ctaaatatga
    24781 tggtcattgt aagaatcttt ctaaagaaga agtagaagcg aatataaaag caggaatacc
    24841 atatgttata agattaagat tgccagagaa tcatacaatt aagtttacag acttagttag
    24901 aggagatatg gaatttaata ctaatgacct agatgaccaa gtattaatga aaacagatgg
    24961 atttccaact tatcattttg cagtagttgt tgatgactat ttgatgaaaa ttacacatgt
    25021 tataagagga gaagaatggg tatcatctac tccaaaacat gtttatttat atgaagcttt
    25081 tggatgggaa gcacctgtat ttgtgcattt accaaatata cttaataaag aaaagaaaaa
    25141 attaagtaaa agacaaggtg atgtagctgt tgaagacttt aagaaaaaag gatatttacc
    25201 agagggatta gtaaactatg tagcattagt tggttggtct cctgaagata atcaagaatt
    25261 gtttacaatg gaggaattag aaaaagcctt ctctgtagaa agagtatcta aaagtggtgg
    25321 agtatttgat actgaaaaat taaattgggt taatcagcac tatataaaag atggtgatga
    25381 tgcttatcta acagacttag caataccatt tttagtagaa gatggattta taacagaaga
    25441 agaagctact aataaatatg atttcttaaa gagtatgata tcagtactta aagagaaatt
    25501 acaatatgta aaagaaatta ctgaacatgc aagtatattt tttggagata aaatagaggt
    25561 agaaactgaa gagggaagag agttcttaag attagaacat atacctactt taatagatgc
    25621 tttgagagaa aaaatagaaa aaacagaggt attaaatgct gagtttgttc aagctatgct
    25681 taaagaaata caaaaagagt acaaaataaa agggaaaaat ttatttatgg gctcaagaat
    25741 aattttgaca gggcaaatgc atggtccaga tttaccaaaa gtaatggaag ttttaggaaa
    25801 agaaacttgc ttaaatagaa tagcttatgt aaaaaataat atattatagt cataaaagtc
    25861 cgctaattta gaagcggact ttttttgtgt tagtatttgg tgaaaatatc tgcttaagaa
    25921 aatgaaggta tttaaggtaa taaatttaat atataaaaag caaagcaaaa atataaataa
    25981 aatatgaata ataaactaat aaaatattaa gcatagaaga agggaatcta ttattttagt
    26041 ttgatttaat tattaagggg gtaattacta tgtatagtaa tattgatgat gttaaaaagg
    26101 aattaaagga gctatgctta gaatatgtta caattttaga aaaattaaaa gatgaaaaaa
    26161 tgattacaga agaaactttt gaaaagtgct cttctcaaaa aaaaatattt ttagaagaac
    26221 aatagaggat ggtaatagta ttattattat gtagtttgaa agatagtacg aaaaatgata
    26281 gtaatactat tatttattgc aattttcaac tataatataa tatataatgt taagaagata
    26341 atttataaaa atacagtgag gtgatgagtg tgaaagttta taatacattg actagaacaa
    26401 aagaagaatt tgttccatta gaagaaggaa aggtaaaaat gtatgtgtgt ggtccaacag
    26461 tttataacta tatacacata ggtaatgcaa gaccatttat aatatttgat actctaagaa
    26521 ggtatttaga gtatagagga tacgatgtaa cttatgtaca aaactttaca gatgttgatg
    26581 ataagattat aaatagaagt catgaggagg gaatatctcc tgaagaagtt gcggctaagt
    26641 atataaaaga atattttgta gactgtgatg ggctagggat aaaaagagct actgttcatc
    26701 ctcaggttac agataatata caacagatta tagagtttat aaaagagttg gaagataagg
    26761 gatatgcata tgcagtaaat ggtgatgtat attttgatac aaataaattt gaaggttatg
    26821 gaaaactttc aggtcaaaaa caagaagatt tagaggcagg ggctagaatt gaggttaatg
    26881 accaaaaaag acatcctatg gactttgttc tttggaaggc aaaaaaagaa ggtgagccag
    26941 gatgggatag cccttgggga gaaggtagac caggatggca tatagaatgt tctgttatgt
    27001 ctaagagata tttaggtgaa acaattgata tacatgcagg tgggcaagat ttaacattcc
    27061 cacatcatga aaatgaaata gctcaaagtg aagctagaag tggaaaaata ttctcaaaat
    27121 actggatgca taatggatat ataaatataa atgatgaaaa gatgagtaaa tcaaaaggta
    27181 atttctttac agtaagagat atatcaaaat tatatgattt agagatagta agatttttca
    27241 tgttatcagc acattataga aatccagtaa actttagtga tgagatgtta aatcaagcta
    27301 aagcaggtct tgaaagacta tataatacta aagaaaaatt agaatttacg ctaagtaatt
    27361 tagtagaatc tcctttaaca gaaaaagaag tagagcttgt taaagaatta gatgacttta
    27421 gacaaaaatt catagatgct atggatgatg atgttaatac agcagacgca gtaagtgtaa
    27481 tatttgaatt agctaagtta ataaattcta atgtagatga aaattcttct ttggagtttg
    27541 ctaaaaaatg cttagatgaa tttaatgaac ttacaggagt attaaatatt gtaaataaga
    27601 aaaaggatac tgtattagat aaagacattg aagaattgat acaaaaaaga actgatgcaa
    27661 agaaaaataa agagtttcaa ttagctgatg atattagaca acaattacta gatatgggaa
    27721 ttgtattaga agacacaaga caaggtgtta agtggaaaag gatataaaaa tggaaaaaac
    27781 tgaattagtt actatgtcac cattagtttt agcatactta ggtgatacag tgtatgagac
    27841 atatataaga gaatatttaa ttagacagaa tacacaaaga aaagttaatg atttgcataa
    27901 actagctata aagtatgtaa aagctaaagc tcaggcaact ataattcatg aaattgaaat
    27961 tgagttaact gaagaagaga gtaaaattta taaaagggga agaaatcaaa agtcaaatac
    28021 ttctcctaag aatgcagata ttatagacta caaacatgca actgggtttg aggcattagt
    28081 gggatatttg tatttaaata atgaaataga gagacttcaa tatattatca ataaaggaat
    28141 caaaattatt gaaagagata tgtaaatatt aaagagtgtt gtcttaataa taagtaataa
    28201 ggcaacactt tttttataac taatgtataa taaatatata gaaattagta atgagaaagg
    28261 ataagaaagt tatgaaagta aaattaatat cacatacacc tgaaccagaa aaagtaatag
    28321 ctatggcagc taagctatgt tattcaccag ttgggacaga cgaaatagaa aaagatttct
    28381 aaaatatatt aaagagcatt cctataacaa atactatggc ggttggaatc tatgagaagt
    28441 tatagatata gaattatgta aattatttgg aaaaaacagg tttgaaaatt agccatgttg
    28501 gtatttctag gttacttaag gaagatcaaa cataaataag ctgagttgtc ttaggtgcac
    28561 tatgtacaac attagaatgt gaagttgaag atttattact tcaaacgaac gatggtacta
    28621 agaaaaacaa tgatgaaatg attaattaat aataaaggta aggaggtagt atatgagttt
    28681 agcagataaa atttttattg atatgtgtaa taatattctt gaaaatggag tatcatcaga
    28741 tggagaggta gttagagcaa aatgggatga tggccagcct gctcatacaa taaagaagtt
    28801 ttgtgtagta aataggtatg atctttcaaa agaattccca atacttacac taagaccaac
    28861 aaacttaaaa gcagcaatag atgaattact ttggatatgg caaaaaaaat ctaataatat
    28921 aaatgatttg aatagccgta tttgggatag ctgggctgat gaaacaggtt caatagggaa
    28981 agcttatgga taccaaattg gacagaaaca taaatacaaa gaaggagaat ttgaccaaat
    29041 agatagagtg atttatgact tgaaatataa tccttatagt aggagaatta taactaatat
    29101 atataatcat aatgatttaa atgaaatgaa tttatatcct tgtgcatata gtatgacatt
    29161 taatgtaact ggaaatagat tgaatgcaat attaaatcaa agatcaaatg atattttggt
    29221 agctaataat tggaatgtaa ctcagtatgc aattttagtt cacatgttag cacaaataag
    29281 tgatttggaa gtaggcgaat tggttcatgt tatagcggat gcacatatat atgatagaca
    29341 tattcctcta gttaaagagc ttattaatag agaatcttat gatgcaccaa aattaataat
    29401 aaatccaaac ataaagaatt tctatgactt tagagttgag gactttacat tagaaaatta
    29461 taaaagtggg ccacaaatca aaaagattcc tgtggcaatt tagaaggagt tatttatgaa
    29521 agttatagta gctgtagatt taaattgggg aattggttat ggtggaaagt tactacagtc
    29581 gattcctgaa gatatgaaat tttttaaaga aaaaactgta ggtaatgtag ttgtaatggg
    29641 gagagaaacc tttaaatcat taccaggaaa aaaagcatta aaagatagag tgaatatagt
    29701 cttgactaga agtaaatctt ttaatgatga tagattgatt gtttgtaatt ctatagatga
    29761 aactcttaag gaactacaaa aatatgaaaa tgataaaatt tttataatag gtggagaaac
    29821 agtatataaa caatttttac cttattgtaa tgaactatat gtaacaaaaa tacagaagac
    29881 atatgaggcg gatagatttt tccctaatat agatattatg tcaaactggg atttgattaa
    29941 agaaagtgaa gtaaaagaat ataacaatat aagatatgtt ttttcaacat atataaataa
    30001 tagtttatag gtttgtatag gagagaaatt taatttatga aaaaggatat atttgtaaat
    30061 agagtttcta atgctttaga aaatgataga ggcgcagttt ttgtaggtag tggtatatcg
    30121 tttagttcga ctgaagttga ttggtttaaa ttactggagc cattagtaaa tgaacttgat
    30181 ataacattag acaataaaag tgatgattta cctatgattg ctcagtacat agtaaataat
    30241 tattcaggaa atagagggcc tttagtaaac gaaatatcaa aaacatttaa tagaaagttt
    30301 aaactaaaca agtatcataa gtatttagca acaactaaac tttcaacaat atggacaact
    30361 aattatgata tgttgttaga agatgcattt tatgattttg atacatcggt taaagtaagt
    30421 gatgattcta ttacaaggaa tagactagaa tatgatgtgg aaattataaa aatgcatgga
    30481 tgtatttcta gatccgattg gaatgaaata gttataactc aagaagatta tgatgatttt
    30541 tcaataaata aaccagctat atctcaaaga ttaagtgaag atttaataaa taagtctttt
    30601 ctatttattg gatatagcta tagagatcca aatataagaa atataatgat aaatgctaga
    30661 aggctaagta aaaaacatac tcaacaacat tatataattt taaaaaagtt aaaagatagt
    30721 gatagtaata ttacgcaaga tgatgtagta agacaagaac tttggtgcaa tgatttaaaa
    30781 cgtttgggaa tttcaacttt atttatagat gaatactcag agttagagga acttttaaga
    30841 gagataagtc aaaaatcaag aggtaaaaca gtatatgtga caggttctca taaagaaagc
    30901 aatatgaatg caagaaaatt aggaaccaag ctagcaaaac ataaagatat tactttacta
    30961 agtggtcaat catctggtat tggctcagag gtagttgctg cgtttactga agagtgtatt
    31021 aagagtaaag aagatattat aaatagaatg aaaatatttc ctaatccata ttcagctgat
    31081 gaaaaatact ctgatgatat tactttaata ccagatttaa agaaatgtag aactaaaatg
    31141 ttgaattcaa cacaagttgt agttgtattt agtggaggta taggtactga agcagagata
    31201 gatgttgcta taaaacaaaa ctgtaatata attcctgtta taacatgtaa agaggataga
    31261 gagaatccag ctataaaaaa aatcttaaac tatagtgata atatgaaaaa tatagaaata
    31321 aaagatgcta cctattataa taaaatagta gatgaagatg gtgttgtaac aatggaggaa
    31381 atttatatat gcatatcaaa aataatgaat taaaacatga cattattaaa ttgaaaaaaa
    31441 gaaatggatt tattgaagtt ggaaaagata aaaaaacgtt ggtaaactgt ttaattggag
    31501 cgaatgaatt agaggattac aaatatgaaa taaaaaaaat agattgtata cttaatatat
    31561 cagaaaaacc agatatagtt tcagatttaa gtactaaaag aagaaaaata agtgaatcta
    31621 tatggtataa gatagttaaa gatacacctt tcgtatctgc tacactgccc atatacttag
    31681 ttagtaaaaa taataataaa atagatagta atgaattatt agatataatt attgagcaga
    31741 tggaaaatgg agttgggatg ataacgattc atccaacagc aaataaacaa atttttgagg
    31801 attctaagaa tagaatggta cctgtcacat ctagaggtgg aggtatggta atacaagatt
    31861 taatctcaaa aaatttttca gaagataatg tgtatttgaa aatattgcca gaaattataa
    31921 agtatgctca aaaacataat gtttgtatca gtctaggtgc aacatttaga tcaggaaaca
    31981 tatttgattc taatgataga gcacagataa cagaaattaa gagtcaaata gaattagcta
    32041 agaggatata taaaagtaat gtcggggtta ttattgaatc accaggtcat tcaagaccaa
    32101 gtgacataat taaaatatcg tcaatattaa aaaaagaagg tttcccaatt atgccactag
    32161 gaccaatacc aacagatatt gcaataggaa tggatcatat cgctgcagct attggtgcta
    32221 caattatggg tatagaagga tgtgcaaata taatatcaac tgtcacacga gaagaacata
    32281 ctggagggag accttctatt gaatccacag ttgaatctat aagaacagca aaaatagctg
    32341 ctcatattat tgatatacat caaatgaatg atattaactt agatatgagt gtatctaagt
    32401 ctagagctaa atctaacacc tgtatatttg ggaaagatac aagatattgt gacagatgta
    32461 gagaactgtg tcctttgtgc attagataaa aattatattc aagttaattc aataaaaaag
    32521 attggataaa aatacatcgt atagctagtt tttcacaaca gagtcaaaga tatgtcaaat
    32581 tagagcaatt tgaatacata attcctccag aaatagaaaa aatagaaaaa gcaaaagagt
    32641 tatttataaa ttcaatgaaa aaataccaag aggattatga taaattagta gagatactat
    32701 ttgaaaatca ttataacaaa ctaataaaag gtggtaaaaa cgagaaaaca tcaaaaagac
    32761 aagttgagaa gaaagcaata gaagatgcta gatacgtatt tccaaatgcg tgtgaaacaa
    32821 agatggtatt tactataaat gctagaagtt tgtttaattt ctttgaacat agatgttatg
    32881 aaaatgggag ttgtctagaa ggaactatga cttgtggaga tatagttcaa gttagaaaaa
    32941 aatttaagaa cttgtaggag gtgaataatt tggcgagtat agaaggaaga aatccggtaa
    33001 tagaagcaat aaaaagtgac agagaaatag ataaaatatt aattgcaaat tcagctaaag
    33061 aaggttcaat taagaaaata ataggaatgg ctaaagataa gaatataatt attcagtatg
    33121 ttgataaaca taagctagat gaagtaagta caagtcattc acatcaaggt gtaatagctt
    33181 atgcaagtga gtataagtat tatgaattag atgagctaat agatttagca aagaataaag
    33241 atgaagaccc atttttcatt atattagatg agataacaga cccacataat ttgggttcaa
    33301 taataaggac agcagatgca gtaggagcac atggagttat cattccaaaa agaaggtctg
    33361 tacacataac tcctgtagtt gccaaagcat ctgctggagc agtagaatat atgccagttt
    33421 gcaaggtaac caatatagtt aatacaataa aaagactcaa agaagaaggg ctgtggattg
    33481 ctgcagctga tatggatgga gaaacttttt ataaacaaaa tcttactggt cctcttggtg
    33541 tagtaattgg aagtgaaggt tttggaatat ctagattggt taaacaaaac tgtgacttta
    33601 tagtaaaaat gccaatgata ggtaatgtta catctttaaa tgcatctgta gcaggtggaa
    33661 tacttttata tgagatattc agacaaagac tggataaaag taaataggca tgaaaaaaaa
    33721 tataaatcat tacttaataa tagatggcta caatattata aatgcgtggg acaatttgaa
    33781 agaactggct aaagaggact tagaagattc tcgagaaaag cttattgatg acattataga
    33841 attttctgag tttatgggtt ataagactat aatagttttt gatgcttata atgttaaaaa
    33901 ttctcgagaa aaagttgaaa aaagaaaaca cataacaata gtttatacaa gagaacacca
    33961 aacagcagat agttatatag agaaatttat aacttcttta tcaaagtatg atgatgtaaa
    34021 agtagccact aatgactatg cagaacagca aatgatttta ggaaaaggtg ctactagaat
    34081 gtctgcaaga gaattaaagt tagagttaga tcgttccaag aataaaatga aggaaaaaaa
    34141 tatcggtttg aggaaaaaaa ttcagcgaaa ttggttggag gaaaggttag ataaagaaac
    34201 attgtcgaaa cttgagaaca ttcgtagaaa gcgttgaaat tactaggttc tttagactat
    34261 aatataccta taaatttgtt ttatatatgg gggagattta catgttggta gcaaaagaaa
    34321 aaagttatga gttagtggac aattgtcagc aggatgagta taacattgta ttaagagcaa
    34381 gtgaagggga taaaatagca ctagagtaca ttattactaa atacagaaat tttgtaaagg
    34441 caaaagcaaa gtcatatttt ttaattggag cagataaaga agatataata caagaaggaa
    34501 tgataggcct ttataaggca gttagagatt ttgatggaag taagaccaat tcatttaaat
    34561 gttttgcgga gatatgtata acaagacaaa taataactgc tataaaaact gcaacaagac
    34621 aaaaacatat acctttaaat tcatatgttt ctttgaataa accaatatat gatgaagagt
    34681 cagacagaac acttttagat attatagcta caagtatagt aacagatcca gaagagctta
    34741 taattagtaa agaagagtta aaaaatatag aatcaaaaat gaacgaattg ttaagtgact
    34801 tggaattaga agtactagaa ttatatttaa atggaaagtc ataccagttt atagcggaca
    34861 agcttaaaag agatgtaaag tcaatagata acgctttaca aagggttaag agaaaattag
    34921 aaaaacatct tgaaaacaga aatgattaat agtaaaatac ttaccataag cttattgtga
    34981 gcaaaattaa tttatatcga aagataaggg aggaaattca aaatggctaa agctaaatac
    35041 gaaagaacaa aacctcatgt taatataggg acaataggac acgtagacca cggtaaaact
    35101 acattaacag cagcaataac aaaaacatta tatgacagat atcaattagg agaagcagta
    35161 gatttcgcaa acatagataa agctccagaa gaaagagaaa gaggaatcac aatatcaaca
    35221 gcacacgttg agtatgaaac accaaataga cactatgcac acgttgactg cccaggacat
    35281 gctgactacg ttaagaacat gataacagga gcagcacaaa tggacggagc aatattagtt
    35341 tgttcagcaa cagatggacc aatgccacaa acaagagagc atatactatt atcaagacaa
    35401 gttggagtac cata
//
LOCUS       DAMKPG010000032        34849 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMKPG010000032 DAMKPG010000000
VERSION     DAMKPG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.035"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(184..840)
                     /locus_tag="PC621_001695"
     CDS             complement(184..840)
                     /locus_tag="PC621_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4108855.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(895..1524)
                     /locus_tag="PC621_001696"
     CDS             complement(895..1524)
                     /locus_tag="PC621_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF4108856.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1655..2296)
                     /locus_tag="PC621_001697"
     CDS             complement(1655..2296)
                     /locus_tag="PC621_001697"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF4108857.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2305..2976)
                     /locus_tag="PC621_001698"
     CDS             complement(2305..2976)
                     /locus_tag="PC621_001698"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF4108858.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2981..3865)
                     /gene="rsgA"
                     /locus_tag="PC621_001699"
     CDS             complement(2981..3865)
                     /gene="rsgA"
                     /locus_tag="PC621_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF4108859.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3963..4700)
                     /locus_tag="PC621_001700"
     CDS             complement(3963..4700)
                     /locus_tag="PC621_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF4108860.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4808..6823)
                     /gene="pknB"
                     /locus_tag="PC621_001701"
     CDS             complement(4808..6823)
                     /gene="pknB"
                     /locus_tag="PC621_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF4108861.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6840..7592)
                     /locus_tag="PC621_001702"
     CDS             complement(6840..7592)
                     /locus_tag="PC621_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF4108862.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7612..8643)
                     /gene="rlmN"
                     /locus_tag="PC621_001703"
     CDS             complement(7612..8643)
                     /gene="rlmN"
                     /locus_tag="PC621_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF4108863.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8651..9976)
                     /gene="rsmB"
                     /locus_tag="PC621_001704"
     CDS             complement(8651..9976)
                     /gene="rsmB"
                     /locus_tag="PC621_001704"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF4108864.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(10038..10739)
                     /locus_tag="PC621_001705"
     CDS             complement(10038..10739)
                     /locus_tag="PC621_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF4108865.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10808..11560)
                     /locus_tag="PC621_001706"
     CDS             complement(10808..11560)
                     /locus_tag="PC621_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF4108866.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            complement(11570..12499)
                     /locus_tag="PC621_001707"
     CDS             complement(11570..12499)
                     /locus_tag="PC621_001707"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF4108867.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12514..12954)
                     /gene="def"
                     /locus_tag="PC621_001708"
     CDS             complement(12514..12954)
                     /gene="def"
                     /locus_tag="PC621_001708"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF4108868.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12972..15497)
                     /gene="priA"
                     /locus_tag="PC621_001709"
     CDS             complement(12972..15497)
                     /gene="priA"
                     /locus_tag="PC621_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF4108869.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15736..16935)
                     /gene="coaBC"
                     /locus_tag="PC621_001710"
     CDS             complement(15736..16935)
                     /gene="coaBC"
                     /locus_tag="PC621_001710"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF4108870.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16940..17206)
                     /locus_tag="PC621_001711"
     CDS             complement(16940..17206)
                     /locus_tag="PC621_001711"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF4108871.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17209..17826)
                     /gene="gmk"
                     /locus_tag="PC621_001712"
     CDS             complement(17209..17826)
                     /gene="gmk"
                     /locus_tag="PC621_001712"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF4108872.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17856..18737)
                     /locus_tag="PC621_001713"
     CDS             complement(17856..18737)
                     /locus_tag="PC621_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF4108873.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18741..19571)
                     /locus_tag="PC621_001714"
     CDS             complement(18741..19571)
                     /locus_tag="PC621_001714"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF4108874.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            complement(19858..20892)
                     /gene="ltaE"
                     /locus_tag="PC621_001715"
     CDS             complement(19858..20892)
                     /gene="ltaE"
                     /locus_tag="PC621_001715"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF4108875.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21188..22963
                     /locus_tag="PC621_001716"
     CDS             21188..22963
                     /locus_tag="PC621_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF4108876.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23178..24830)
                     /locus_tag="PC621_001717"
     CDS             complement(23178..24830)
                     /locus_tag="PC621_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF4108877.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(25129..26340)
                     /locus_tag="PC621_001718"
     CDS             complement(25129..26340)
                     /locus_tag="PC621_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF4108878.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26624..27157)
                     /gene="pyrR"
                     /locus_tag="PC621_001719"
     CDS             complement(26624..27157)
                     /gene="pyrR"
                     /locus_tag="PC621_001719"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF4108879.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27516..28463)
                     /locus_tag="PC621_001720"
     CDS             complement(27516..28463)
                     /locus_tag="PC621_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF4108880.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(28432..28878)
                     /gene="lspA"
                     /locus_tag="PC621_001721"
     CDS             complement(28432..28878)
                     /gene="lspA"
                     /locus_tag="PC621_001721"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF4108881.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29188..29967)
                     /locus_tag="PC621_001722"
     CDS             complement(29188..29967)
                     /locus_tag="PC621_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4108882.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(30017..30499)
                     /locus_tag="PC621_001723"
     CDS             complement(30017..30499)
                     /locus_tag="PC621_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF4108883.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(30683..32104)
                     /locus_tag="PC621_001724"
     CDS             complement(30683..32104)
                     /locus_tag="PC621_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF4108884.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(32250..33020)
                     /locus_tag="PC621_001725"
     CDS             complement(32250..33020)
                     /locus_tag="PC621_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108885.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(33025..34227)
                     /locus_tag="PC621_001726"
     CDS             complement(33025..34227)
                     /locus_tag="PC621_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF4108886.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT        11117 a         6391 c         3510 g        13831 t
ORIGIN      
        1 ttctaaagat ttaaatatat ttagaattaa tatataaaaa aaagtagttc ttattgtatt
       61 aattaaatca atgatttgaa atataaaatt aatatcaata aaacctactt tttaattttt
      121 taaatattta tttttaattt atcttgtttt taatttactt tgattttaaa atgtccttga
      181 cttttatttt ataaatgtac atatttcttc tactggtttt ttagatggac tcttcaaatt
      241 gtcctctggc actccaagag ctaacattgc acctaaaaaa tactctccct tttcaaaacc
      301 agtttctgct tctaaaacag cttctatctc atctgctgca tagttttgac ttgtcaacca
      361 acaagaacca tatccaagtt caattgctga tagtgtaaag ttttcaattg aagcacctag
      421 actctgcata cctggatttc ttataaataa tttatctata gtctccttag gtgcatctat
      481 aagctctaat tcataatatc ctgatggatt atataccttt gtaaaaacta atactaaaac
      541 tggagctttt aagtaaaata gtgtaaagtt tttaacaaat tttctaaatc tattagcctt
      601 atccactgat actttatcca tttccacaag tatttcttgt tgctttttag ttatcacatc
      661 tgctatcttt tccattaaat cacggcgttt tataacaaca aaatgccagt tttgaatatt
      721 cttgcctgat ggtgctgcac ctgctgcttt tatcattttt aaaatatctt catctgatac
      781 atcttgattt ttaaactttc ttacactttg tcttttaaaa atagtatctt gtaattccat
      841 ttttaaatcc ccccataact tttttattat tttaatataa tttttaaact catcttattt
      901 taatatatca tgttctaaca aatctttaaa tgaatctata aaataatcat atacatctac
      961 attcccaaac ccatatttgg catatataaa aggtatacct acaaacttag cactatttgc
     1021 atcaccttgc gtatcaccaa catagattgg atttattaat ttatttcttt caattataag
     1081 tttattattt tctcctttat gcaaaccagt attaccagga cattcaaaat ctataaaata
     1141 ctgttctagt ttatgtgctt ttaaaaaaga ttctatatat ccagcttgac aattacttac
     1201 tataaataat ttatattttt tagatagtaa tttaagagta tcttctatat catcaaataa
     1261 tttagctcca ttttcagata aatagtcaca ttctttcaaa gaacattcat tcaataaatt
     1321 cattctttct tcttcactta aatttggaaa taaacgattt gctatttcct caaaagaaag
     1381 ccccataaga cttctaaata aatcttttgt gacacttaat tctatttcct tatgtttact
     1441 taaaacatcc tgccagactt tgcatacttc ttcagttgaa tcccacaatg ttccatctaa
     1501 atcaaaaata atactatcta ccacaaaaaa tcccccttat gtcaaagttt attatatatt
     1561 tataattata tactaaaaag cagatacaat cataaatata ttttacttga ttgtatctgc
     1621 tctacaatat aaaaccaatt gtcaaaatca aattttataa atttctaact acaagtacac
     1681 agccttgctc tactttaatc ttacaagaat tactcaacat aacatttgat attccaatag
     1741 gtacagagta tttcatataa taattatcga gagggtattc taagccagta agtgttacac
     1801 ccttagcatc accttttata gctgttacag aaacagtgtc ccctgggttt ccatacaatg
     1861 aaatttcctc attttcaact atatacatct cttctttatc agaaagtatc ctagaatata
     1921 taccatcttc ttttaaataa tataatagct ttatatttgc aagagtgtgg tcaatccttc
     1981 cccctaaagc accaaaaaaa tcaatatgat ttgctttaag tgtcttagct aaaaaaagac
     2041 aaagctctgt atcagtctca tcttttttag atggaaattt ttcaaattta acacctttat
     2101 ttttatagaa gtttatcttt tcttcttcta ctgaatctaa atctcctaat atataatctg
     2161 gcattatttc cattttataa gtgtgatttg cccctccatc tgcacatatt atgcagtcat
     2221 aacattcctt aattataata tctcttgtaa cttcgtaatc ttcaatttcg ccatttaaaa
     2281 caatacagat tttcattatt aaatttaaac taattcttca agagaagcat tctttctaaa
     2341 taaattaact gtttcttgaa tgtcatcact tccaaatatt gcagaacctg ctactattac
     2401 attagctcct gcttgtacaa cttcggctac gtttttaggg cttataccac catcaacttc
     2461 tatatcaatg tttaatcctt gactatctat aatagctttt aactctttaa tcttatctaa
     2521 aacacaagga ataaatgatt gtcctccaaa tccagggtta acagacatta ataataccat
     2581 atctaaatca ggtagtacat gtttaatcgt atctactggt gtagctggat taagtactac
     2641 accagctttt attccatgtg attttatatt ttgtatagtt ctatgtagat gaacacaagc
     2701 ctcctggtga acagttatta tatcacatcc tgcttttaca aattcttcaa tatactggtc
     2761 tggattttct atcataagat gagcatcaaa taccatattt atattttctt tctttaaact
     2821 tttgactaca agtggtccta atgtaatgtt tggtacaaag tgtccatcca ttacatcgat
     2881 atgaagatat tcacatcctg cactttctac ttttcttaca tcttctaata attttgcaaa
     2941 atctgctgat aatattgatg gtgctaattt aatcattttt ctaatacctc ctactattat
     3001 tttgtcttat ttcatgtagc agctgtatat aactatcata tctttcttta gatatttctc
     3061 catttgtaac agcttctttt atagcacaat taggttcatt ttcatgaata cactttgaac
     3121 caaacttaca atcattaaat ttatcaaatt ctataaaata atctttaagt tcaacttcct
     3181 ctatatcttc aagtgcaagg gaactaaaac ctggtgtatc tgcaaccatt ccaccaaatt
     3241 ccaattttaa aagttcagcg tgacgagtag tatgttttcc cctttttatt ttatcactta
     3301 ccactcctgt ttgaagttta aaattttcat ctatttcatt taataagctt gattttccaa
     3361 ctccagatgg tcctgcaaac actactacat tttcttttaa ttcttctttt actttatcta
     3421 tatttaactt agtaatattg cttacaggaa tgactttata gccactaagt tcatatatat
     3481 ttttaacagt ttctaatgta tcattatcat ctaaatctgc ttttgtaagg attattacag
     3541 tttctaaatt ttctttttct gccaaaacta taaatctatc taaaagagat aagtttggtt
     3601 taggattttt tattgcgaat actattaatg ctttatctac atttgcaata ggaggtctta
     3661 taagttctgt atctctactg tcgatttctt ctaagatacc ctttttttca tcttcatcaa
     3721 caatgcttat tttgactcta tcaccaacca aaggtgttat tttctgcttt ctaaatatcc
     3781 ctctagcctt acattcataa ataccattat aagtatctac ataataaaat ccactaatcc
     3841 cctttataat ttttccttct agcatcaaat gcctcccttt ataaaattta tataaacaca
     3901 tactgtttat gaatagtata tcgtgttatt ctttcctatt tttatataat accacatttt
     3961 ttttaaataa caaatcctcc gtttggacta attacctgac cagtgataaa atttgatttg
     4021 tctgatgcca agaaaatagc acaatttgct atatcttctg gagttcctag cctcattaga
     4081 ggggtttctt ccacaagagc gtctatatct tcctcattat atccagataa catgtcagta
     4141 ttaatgacac ctggagctat gctatttact ctaatatttg atggtcctac ttctttagct
     4201 aatgcctttg tcatacctat tatacctgct tttgttatag aataatgcac ttcacaagac
     4261 gcacctgata taccccagat tgaagatata ttaattatat ttcctttttt ttctgatatc
     4321 atgtatttta aagcaacttg cgaacaataa aagcttcctt ttaaattaat attcatcata
     4381 ttatcccaat cttcatcggt tatatctgta aacaacttat cttgacttac accagcattg
     4441 tttacaagta catccaatcc tccaaattct tttatacaat aatctaccat attctctaca
     4501 tcttctctat ttgaaatatc agccttaaaa agttttacag aaaaattttt ttcatttaat
     4561 atagtataca actctttagc ttcattttct gacttattaa aatttactaa tacattgtaa
     4621 ccttcttttg caaaagcttt agacatagct ttaccaatcc ctctagctcc tcctgtaatt
     4681 aaaacggttt tcttcttcat aaatactttt ctccttatat tataaccaat tcagtatttt
     4741 atacaaaaca aactgccctt taaagaaaat attttctata aaaggacagc tcttatttta
     4801 ttaatattta ttctgtttta ccaatgtttg cgccagttcc tgatgaagtt cctccattat
     4861 ctcctgagtt tgagcctcct gaactatctc cattgtctcc tgagtttgaa cctcctgaac
     4921 ttcctccatt atctcctgag tttgaacctc ctgaacttcc tccattatct cctgagtttg
     4981 agcctcctga gcttcctcca ctatttcctg agtttgaacc tcctgagtta cctccattat
     5041 tgcctgagtt tcctccacta tttcctgagt ttgaacttcc tgaatttcca ccactgttcc
     5101 ctgagtttga accaggttct gttggtgtat tattctctgg tgttgttggt tttttatctg
     5161 gtactgttgg agtctgtgta tttgattttt ctgaaccttt gcttacatat aggtttacct
     5221 catcaccttc ctgagctctt gatgagccag cacctggact ctgacttaaa actgtacctt
     5281 ctttatatat tgagctatat tcatacttaa cagttcccac ttttaagtta ttttcttcaa
     5341 taagttgttt tgcattactc aaagtaagtc ccaccacatt aggaacagat gcctgacttg
     5401 aacctttact tacgacaaca tttatagtat cacccttttt gacatttgta cttccacctt
     5461 caggtgtctg tgatattata caattttcat caacttcact attatactct tcacttttta
     5521 cactcaggta taatccttct ttttcaacag cactttgagc ttcttctaat gtcatattta
     5581 caagattagg aacagtcaaa gatttattgc caaaccctcc agcaaataaa aacttatatg
     5641 ctaagaatac ttgagcacac aatatcagta tcaaaacagc cgcaattact tttaatcttc
     5701 ttctagattt tgggctttcc tgttttttct tctttttctt agttctctta cttggagtac
     5761 tcttaggtct ctgattcttt ttagctctca ttatctcttc ctcttcttct tcttcttctt
     5821 catcttcttc ataaaaatca tcatagtaat cttcatcatc atccaaatca gctacttcaa
     5881 caggcttaac cactttttca ggagctggtt ttgcaagtgt tggatttaca actttattaa
     5941 tttctttttc atcaattttc ttagttgcaa aatcatcata ttcttttata aaatctaaat
     6001 caatattttt ttctatatac tctatatctt ctatcaattc ttcagcagtt tgatatctat
     6061 ctgcgctaga tttttctgta agctttttta ttgtagttct tacactttga ggaatcctaa
     6121 ctttttcttc tgatgtaaag tctatatcat cattaatgtg ttgaagtgct atagaaattg
     6181 gactatctcc cctaaatgga acttttccta tcaacatttc atataaaact atacctagag
     6241 aatacaggtc agcattatta gttacaaatt tacctttagc ttgctctggt gaaaaataat
     6301 gcactgaacc tattatacta ccaatattag tcattgttga atttgaaaca gctttggcta
     6361 taccaaaatc agccactttt actactcttc cttcatttga tatgagaata ttatgaggtt
     6421 ttatatctct atgtataatc ccctttttgt gcgctgcact aagtgccata gctatttgtt
     6481 ttgttatatc taatgcagta tattcatcta atataccttc atcttgtata atctctttta
     6541 gattcttccc atctacaaac tccattacaa tgtagtgaac ttttccatct tcaccaacat
     6601 cataaacatt tactatattt ggatgtgaaa gacttgctac tgcttctgcc tctcttttaa
     6661 actttgttag aaattcatca tcatctacaa attcaggtct aagtacttta agtgcaacag
     6721 tcctgtttaa taatctgtct ttagcctcat atacaaaggc cattcctcca tcaccaatct
     6781 tcctgatgat ttcatatcga tttcctaaaa ttgtatctcc cacaatatca ccgtcctaat
     6841 tatactttta tcactataac cgaaacatta tctttaccag aaacatcatt ggccatactt
     6901 atcaattctt cacttatatt actagttctc tcatattcat aatctaatat taaatctctt
     6961 atatcttcat catcaataaa cccagttaat ccatctgtcg caagaagtat tatatcactt
     7021 tttttgattt ctttcttaaa aatatctaca acaaccatat catctgttcc aattgctcta
     7081 gttattctat ttctctgtgg atgtcttctt gcctcttctt ctgtaatgac atgggccatc
     7141 ataagttctt caaccacgga atggtctata gttattttat caaatttttc atctgttaac
     7201 aaataacatc tgctatctcc aacatttgcc acatataaga cattattata cactatagcc
     7261 gtaactaatg tagttcccat tccttcaaat tctatatctt ccatagattt tttgtgcaca
     7321 attgagttta cattattata agcctgtttt aatatatcat ctatatagtc tatttttaca
     7381 ttatcatgtt gtaataagtt ttcctttaga aaatcaatta tattttctac tgctaattta
     7441 ctggcgactt cacctttttt atgtccaccc attccatcag ctatggcaaa tattcctatt
     7501 ggaccatgtt ctgtatctat aacttctccc tcacaatagt cttcattgtt ttttcttatt
     7561 tttcctatat gggaggcaca actataaacc atacgacttc ctccttacaa attacttgta
     7621 ttttcttctc aattgaccac aagctccact aatatcagaa cccatagaca tcctaactgt
     7681 ggcaggtata ttgttcttct ccaaactatc cctaaattta taaataaatg ctttatctgg
     7741 cttttcgtat tctctttctt caactttatt tattggaatt aaatttacat gacatagcat
     7801 accttttagg agcttagcta atgcctttgc ttctttttct gagtcattta ctccttttat
     7861 aagagaatat tcaaatgtaa cccttctatt tgtctttttt atataatatc tacatgcatt
     7921 caatatttct tcaatagaat aagcatttgc tacaggcata atttttcttc gttcttcatc
     7981 atatggtgaa tgaagtgata aagctaaatt tatagctatt tctaagtctg ccaactcata
     8041 cattttaggt actattccac aagttgaaag agttatatgt ctatacccta tattaagacc
     8101 atttttttcg ttaattattt ttaagaattg ttttgtattt tcaaagttat caagtggctc
     8161 accactcccc atcaatacaa gatttgatac cctcttacca gtatcttctt gaattttaat
     8221 aacttggtct aaaatttccc atggctctaa gtttctaatt aatccatcca ttgtagatgc
     8281 acaaaaatta catcccatcc tacaacctac ttggttagaa acacagacag taactctact
     8341 atcataatcc atcatcacag tttctattat atttccatca tctaataaaa acaaatattt
     8401 ttttgtatta tctaccttag attctaactt taattctata tctatattcc ctatacatga
     8461 aacctcttct aatttatttc tcaaactttt tggtatattg ttcatatcat caaaagtttt
     8521 tgcaccctta tatatccaag agaatatttg acttcctcta aagggctttt catctatagt
     8581 tttcataaat tctttaagtt catcttcagt aaaatttttt aatactatct ttttcttttc
     8641 cattctctca ctaccttatt ctctttaatt ttgctatgaa aaaaccatct ataccatgaa
     8701 tattaggata tatctttaaa tatcctttat cttgattgtc taagtctaca tttactgtat
     8761 caattggaac aaattcaaag ttgtgatttt cttcaacaaa agaagtaact atatttatat
     8821 tctccacatc ttgaattgta catgtactat atactaaagt tccaccaact ttgacatatt
     8881 tagatgcatt ttctaaaatt ttcttttgta tagaagtgat gtcttttaac tcttcttctt
     8941 tcttatactt aatttctggt ttacgtctta ttataccaaa accagagcaa ggaacatctg
     9001 ctaaaacata atcaaattta cttatactat tttcatcaat ctcagaagca tcaaatcttt
     9061 ctacaaacac atttttaagc cccaatctat ttacagtaga gtttataagc tttagtttat
     9121 gctcaaaaat atctctagct actacttgac ccgtattgtt cattaaagta gctaagtgag
     9181 tagatttccc tccaggagca ctacatacat ccaaaacaaa tgaaccttct tttggattaa
     9241 ttactttccc tactatcata gaacttatat cttgaattgt aaataaacca gccttaaata
     9301 attcattttt ttctatattt ttcatttttt cgactttaat agcttcttct accataggaa
     9361 cttttaaaca tacaataccc atttcactta atttttcaat aagttcttct ctacttattt
     9421 taagtgtatt cgttcttata tagatactag gcttttcatt atttgcttcc aacaaatctt
     9481 cagcaaattc ttgtccaaat ttatctatcc agttttttat aatccaagag ttataggaat
     9541 actttataga taagtatttt acactatctt cctcagttat gtccattata gtctctttag
     9601 ctcttatttc atttctaaga atagcattta tgaaaccaga agatttttta tcatatttct
     9661 tagataattt tacagtttca ttaactgctg cataatctga tatactatct aaaaataaaa
     9721 tctgatatgt tcccattctt aaaaagattt taacataagt tgacattttt tttaccttta
     9781 tttttgaaag tttattgatt atataatcta aataatattt attttctatt actccataaa
     9841 ttaactctgt tgcaagtcct ctatccatat cacttatttc taaattttta aaatgcttat
     9901 ttattgcaat atttgaataa ttgtcatttt tttctatatc acataatact ttaaatccaa
     9961 cttctcttgc atccatataa atttctccct tttattaaac ataaaaaagg gttgtttcta
    10021 aacaacccac tttactttta gtcatttctt ctttgagcta ttagtaacaa tctaagtaat
    10081 tgaagtattg aaaccaatgc tgctgcaaca tatgttaaag ctgctgctga aagtactctt
    10141 ctactttgtc ttgactcact ttcatctata atacctaaat tcccaagttg aacgattgct
    10201 ctacttgaag cattaaattc tacaggcaat gttattattt ggaataacac tgaagccgaa
    10261 aacaatagta taccaatttt taaaaatgga ccagccataa taaatcctaa aaatattaaa
    10321 aaccaagata tacttgaagc aaaattcact actggtacta aactacttct tatttgaaga
    10381 ggcgcataac ctttagcatg ttgtatggca tgaccacact catgtgcagc tactgcaaca
    10441 gaagttatag atgttccata atatacatct tctgacaacc ttacagcctt tcttcttggg
    10501 tcataatggt cacttagatg ccctctaacc atttctatct ttacatcata caaaccattt
    10561 gaatcaagta ctcttcttgc tgtttgctct ccagtatatc ctcgacgtgt atttactctt
    10621 aaatacttat ttgttgttga actcacttta aattgtgcat atatagtaag taatatagct
    10681 ggaattaata caaccattgt tgggtcaaat ccccaaaaac cgccatatac agggtacata
    10741 aataacactc tcctaaaata tatttttatt atacatcctc ttaatattca cttaacttaa
    10801 aaacagttta attacaaatt aatatatttt ttcctgttaa aaagtctata gctttttcaa
    10861 tatccatcat gtctacatta gtatttacac aaggtccatt aggtcttttg ttaaatacac
    10921 ctaaaacagg aattttctta atatcttgta ttccagatgt caaatctctt tcacatgcaa
    10981 cagctacaac agctttaggt ttattatcca ttataatttt tctagctagg gttcctcctg
    11041 tagctacaaa tatttttaca ttagtttttt cctttagctt aattaattct cctacgttgc
    11101 atcttccaca ttctttacaa ttatctatat cattagtaac ttttaattta caactattct
    11161 cttgaataca atgaggtatt aaaattatta tatcctctgg attaaaatta tatttattac
    11221 tgtatatata ttcattattt aactttatat atattcttct aatatcactt tttgacatac
    11281 caaatgatga tgcaatgaaa gatataatag gaaataatcc actgacaata ttaaaattta
    11341 ttttcattat atttgagtta acatactttc ctttaattac tctataagta actgctgttg
    11401 aacatagtat tataagtatt agtgatatta ttataatatt tatcatcaag gagaatattt
    11461 gagttaaact tattataaaa aaattaacaa ctcctatcaa aattcctaat attaaaatca
    11521 aaatcccaac acttgttaaa tacttcttta tatctgtcat acttttcacc taacccaata
    11581 ctaaaccagt ttctatagta ttccctttta tatattcacc aaccaacatt ctctttttat
    11641 tcggcatctg aatttcttca attaaaagta cattatctat agtacttact tttatcccat
    11701 ctttgcttac atcaattatt gtacctacat cttttgtgct tttttcatct aacactttag
    11761 ttttccaaac tttcattatg acatcattat agatagtata agcacttggc catggattta
    11821 ctcctctaac taaattgtgt atttctctgg cactttttga aaaatctatg ttacctaatg
    11881 atttattcat tattggcgca taggaaaatt cttcatgatt ttgtacttct cttggagcat
    11941 ttccttcttc aattaatctt aatgtttctt ttaaagtttc tgctcctata ttcatcattt
    12001 tatcatgaag ttctcctgct gttatatttt catcaagatt tacttctgtc tttagtatca
    12061 tatctccagt atctaaccct tcatccatat acatagttgt aacaccagtc ttttcttcac
    12121 catttattat tacccaattt ataggtgccg cacctctata ttttggaagt aaagaaacat
    12181 gaacatttat acatccaagt ttaggaatct ctaatatttc ttttggaagt atctgtccaa
    12241 aagctacaac tactattaca tctggattta aagatttaat tttatctata aattctttat
    12301 ctctagcctt tactggttga tatacaggta tattattttc aattgccagt tcttttactg
    12361 gactcatacc tagtttttta cctcttcctt ttggtttatc tggttgagtt acaacaccca
    12421 atatttcata cttctcatct attatttttt gtaaacatgg aactgctata tcaggagttc
    12481 ccataaatac tattttcatc aaatcaactc ctactacttt tctattttgt caacaaataa
    12541 aattccatct aagtggtcta tctcatgaca aaatgctcta gctaaaagtt cttctccctc
    12601 taactctatt gtttcgccat ttctatttaa agctctgacc tttacataat ttggtcttct
    12661 aacatttcca gcttctccaa caacacttaa acatccttcc tcatctattt gctccccaga
    12721 tgtttctatt atttcaggat ttataagttc tataagctct tctccaatat ctattactac
    12781 tactcttttt aatattccaa cttgaggtgc agccagtcca acaccatctg catcatacat
    12841 agtttctgcc atatcatcta ataattgtat tatcttttca tcaatttttt caaccttttt
    12901 tgattttttt cttaatactg gttctcctat ttgaactatt tgtcttaaag ccattataat
    12961 tcctcctaaa tttataatac actgtttgga ttaatatcta tagatacatt gatattctta
    13021 tcaaatatca aatctcgttt tgttatacat atatatttta ttataccctt taagagatta
    13081 atttcaatat tttcatcttt aaagagtatt tgccatctat aattttgatt tatttttgat
    13141 attgaacatg gatttggacc taatataaat cctaaatcat ttatacccct tttttctaat
    13201 aaataaatca atgatgcatg catatttttt atatttttta tcactaatct ttcatcttca
    13261 ccactaacaa ctacacttaa catattatta aaaggagaat atccaaatgc ttttcttatt
    13321 tttatctcat cttcataaaa accctcataa tcatattcaa tagcatgctt aatagcatag
    13381 tgttcagtgt cataagtctg aagtactact tttccctctt tatcagctct ccctgctctt
    13441 ccagagactt gagttatcaa ttgaaatgta gtttcaaaac ttttaaagtc tggaaaattt
    13501 aatatcatgt ctgcagacaa aatacctact aaagttacat tctcaaaatc cagcccttta
    13561 cttaacatct gtgttccaat aagtacatct gcttctttat ctttaaattt atttagtatc
    13621 tcatctaaag cacctttttt tgaagttgta tctttatcca ttctcaaagt ttttatatct
    13681 ggaaaaatat attttaattc ctcttcaatt ttttgagttc caactccaaa tggcttaaca
    13741 taagtacttt tacattcagg acactctttt ggaatttctt tctcatatcc acaatagtgg
    13801 catcttccag tattgctttt cttatgataa gttaaagata tatcacaatt ttcacattga
    13861 aacacatatc cacactttct acaagataca aaatttgcat aacctcttct gtttaaaaat
    13921 agtatcactt gattattttc ttcaatcgca taacgtattt ctttttgtaa tttcaaacta
    13981 aatatacttc tatttccttt atctaattca tctttcatgt ctaccacttc aatgtttgga
    14041 agaggcttat catttgctct actttttata tttataagtt tgtattctcc agattttgcc
    14101 ctataatact cttcaattga gggcgttgct gaacctaaaa taagagttat attatttttt
    14161 aatgccataa atctagcaac ttctatagca ttaaattttg gattcttctc agacttatat
    14221 gatgactcat gacattcatc aattattatt aagcctaagg aattaaaagg tgcaaataaa
    14281 gctgacctag ctccaattaa cactcttact ttacctgctt tgacagcttt gtaaacatca
    14341 tgtttttctc cttcagatag tttactgtgg aacactccaa ctaaatctcc aaacctactc
    14401 tttagcctat ctattgtttg aggagttaat gctatctcag gtactaagaa tatactgtct
    14461 aagccttgat ttagagcaaa ttctattatt tccatgtaaa cttcagtttt tccacttcca
    14521 gttactccat gaattaagta tggtttttta tcatctacaa acatattcga ctttatctca
    14581 tcaactgctt cttgttgttc attgtttaat tttatacttt ttagtacgga tttgtaaata
    14641 ctttttggct ctctataata atccttaaat tctagggtta ttagcttttt cttacttaga
    14701 gaatttatac tttgttttga agcatttaat aaatccaata aatcatttat ctctacattt
    14761 tcattatttt taagaaaact taatatctct ttttgtttac ttcccacatt tattttattt
    14821 tgctctatat aatcgtcaat cttatcactt tctaaactca aagatatata acatactttc
    14881 ttttcatttt tgtgattttt atattcccaa cataatttta taattccatt tttattcatt
    14941 gtgtacaaag aactatttaa gtttggtata tattttaact tatcaacctt aattttacct
    15001 ttactatcaa tcactttctc tactatttct ctatttttat cacttaaagt tgatattttt
    15061 tccttcaatt caatctcatt taatccactt aaactttcac ctaataggac tactttataa
    15121 ttatttaatt tgtatccctt tggatatatc aaatttatac attctatata agtacacaaa
    15181 tatctatttt tcatccaata aaccaactct aaatcttctt ttctaaatat tggattttca
    15241 tctaatatat caataatttc ttttattttt attttttcat ctaagctatc tgtaaattga
    15301 aatacaaagg cttctgttgg tttattacct tttccaaatg gtactaatat tctgtgcccg
    15361 atttttatta catcagataa aaatacagga atttgatatg taaataaatt atctgtatat
    15421 atagtattac ttctaactat tacctttgca tattttttca ttataaaacc tctacttaaa
    15481 tttaatatat tgttcatatt ttaatatact ttacaagata ttaaaatctc agaatttata
    15541 agcaatttca agttattaat attaacttta ttttaataat taactattaa ttatatttta
    15601 tactttatga caaaataaat gctgaaaact acaatttaac tgacatttgc gagtttttac
    15661 tcgctaaaat taaattgcag ctttcagcca ctttagctgt gataaaatac acaaatatca
    15721 tatttaaaaa aggtcttatt tctcaagcaa cgctttaact ttatctaaaa taacattggc
    15781 tacttcttct tttttcatct taggatactc agtaatattt ccctctacat ctattatttt
    15841 tacaatatta gtatcagctc taaaaccagc accttcttta gttaaatcat ttgccacaat
    15901 aaaatctaaa tttttctttt ttatttttcc tttggcatgt tctataaggt cattagtttc
    15961 agctgcaaat ccaattagta tcttatcttc ttttattttt cctatttcat aagcaatatc
    16021 ataatttcta cttagttcta ttgataaatc atcattcttc tttttaattt ttttgtcaga
    16081 ataagttttt ggtttataat ctgcaactgc tgcactttga attactattt tattttcact
    16141 taaatttgat actactgctt catacaaatc ttgtgctgac tctatttgaa caaatttttt
    16201 aagattttgt ggaatagaaa gatttgtagg acctgatatt aatgttacat cagcacctct
    16261 agctatagct tctcttgcta tagaatatcc cattttacca gaagacctat tagttaaata
    16321 tcttactggg tctatactct ctacagttgg accagctgta actatcatac tttgaccttt
    16381 taaatcttgc tctttagtaa gattagtcaa aacttcctct actattatgt ctggattagc
    16441 taatttgcct gttccaatat ctccacatgc aagcctccca ctttctggtt caataaattt
    16501 atatccaaag ctctttaaag tttgtatatt tttttgaaca attggatttt catacatatt
    16561 agtattcata gctggtgcta taagaacctg tgctttggtt gccataacag tagttgtcaa
    16621 catatcgtca gctataccat ttgcaatctt gcctataaca tttgcacttg ctggaactat
    16681 taagaataaa tctgcttttt ttgctaatga tatatgttca acttcccaag tttttggttc
    16741 ttcaaacata tcttctacta cataattttg actcagcgat tgaaatgcaa gtggtttaac
    16801 aaattctgtt gctgattttg tcattataac atggatattt gcatttagtt tttttaactt
    16861 acttacaaca tcacaagcct tatatgcagc tataccgcca cttatgccaa ttacaacagt
    16921 tttatctttt agcatgacct tactcctctt ctgaaatttg ttggtgttgc tcagcatgat
    16981 gtctagcttc ttctatctct atttcctctt ctgtcaataa tctatatgtt atttttccac
    17041 ttgcaacttc tttagttgct acacatacag gcttttcctt agttttattt gaaacatatg
    17101 gttcttctcc atcaattaat tttcttgctc tctttgaaac agtccctact aaatagtatc
    17161 tgttgtctat tttttctagt acctcattga tagatggttt taacataatt ataattcctc
    17221 cttaaatttc tctattataa tatttttata tctgctaact ttattctttt cagatgatat
    17281 tataccttct atttctttag cagatgtttt tacatcttca ttaaatataa aataatcata
    17341 atccttaatc attttaattt cttcatatgc acagctaaat ctcttctcta tttcagcttc
    17401 tgtttctgta cctcttccaa caattctatt ttttaattct tcaagtgatg gtggtagtac
    17461 aaatataaat actccctctg ggcaaacttc ttttatttgt tttgctcctt gcatttcaat
    17521 ctccagtaat acatcttgtc ctttttctaa acattccatt attgccgctt ttggagttcc
    17581 ataaaaatta tcataaattt gagcatactc caaaaactca cctttttcta tcatctcttc
    17641 aaacttttcc ttacttatga agaaataatt tactccatca acttctccag ttctgggttt
    17701 tcttgtagta gctgatacag aaagttttat tgtatcattt tctttaagta gttctttgca
    17761 aatagtacct tttcctgcac ctgatggtcc tgatacaact agcaataatc cttttttcac
    17821 aaacatttaa gcattcccct tctaatattg tattcttact ctatattttg tatttgttct
    17881 cttatttttt ccaattcgct ttttacttct actactaaat tagttatatt tatatcagat
    17941 gacttcgaac ctatagtgtt agtttctcta ttcatctctt gtattaagaa atctattttt
    18001 ctacctatag agtcgtcttt aaatatagta tttttaagtt gccctatatg acttttaaat
    18061 ctaactattt cttctgttat actgctttta tctgcatata ttgcaacttc ttgtgctaat
    18121 ctattctcat ctattatact tgggtcatcc agtaattcac ttattctcaa attcaatttt
    18181 tctctataat cttctacaac actacttgaa tatttttcaa tctcttctat atgatttttt
    18241 aataaatcac atctcatggc aatatcttca gctaattttt taccttcttc acttctcatt
    18301 tctcttaatt tgattaatgc atcttcaact gcttgattca acattgacca caataaatct
    18361 tcatcttctt ccttttcttc tattttcaca atatcaggaa actttgcaac attcataacg
    18421 ctaatatcat ctataatatc aaatttattt tttatttctt ttagtatgtc tatatactga
    18481 gatgctaatc cctcatcaaa atttaagttt acatcttctt tgcctaataa gtcaaacttt
    18541 atgtataaat ctactcttcc tctttttaca taatttttta ctaaatttct aactttatcc
    18601 tctaaaaatg aaatttttct tggaagacgt atgtttatat ctgaatactt atgatttatc
    18661 gttttacact ctactaaaaa gtaataatta tcatccttgt attctcctct tccaaaacca
    18721 gtcatactta tagccattaa ttaaacctcc aaaacacctt cacatatttt tactgctgga
    18781 cctgtcatat aagagaaatc tcctacatct attttaactg ttccaccttg agacgttaca
    18841 tttacactac tttctaccat attaagtaaa ttgcacacta ttgctgatgc agtcatacca
    18901 gtaccacaac ctaaagtata tccacaacct ctttcccaag tataaacatg tatattatac
    18961 ttatcttcta cctttacaaa atttacattc gttttttcag gaaatatttt attattttca
    19021 atctcttttc catatttaca aacatcatct acatctaatt catctactat aactactgta
    19081 tgaggaaccc ccatcaacat agaagtaact ttaagctccc tatctaaaac ctttatactt
    19141 tcttgcacaa aacgttcttt atttgtaagt acaggaacat ccttagctat aaaacttcct
    19201 tttcccatat tgactcttat agagtttatt tctctattgg taagatttat ttttatatct
    19261 tttataccat ctaatgtatc tactgtaaaa ctttcttttt taactataca attatcataa
    19321 acaaatttac aaaaacatct aaggccattt ccacacatag ctgctcttga accatcagaa
    19381 ttataatata ccatttctac atctgcaact tttgaatctt tcacaactaa caatccatcc
    19441 gctcctacag aaaatcttct atggcatact ttttttgcta aagcagtata atcagatgga
    19501 tttatctggt cgaatcttcc atctattgct ataaaatcat ttcctattcc atgtagtttc
    19561 caaaatttca taagtatcgc tccttcgtaa attaacgctt aaactattat aacataaaaa
    19621 aattcccata aacttataat ttatgggaaa agttttgtaa tgacaactat aatttgagga
    19681 tgtgagggag agttgtcatt acaattaggg taattatatt atgtttttaa gctagttctt
    19741 tctatgttta tatattaata tttctatatg aattttatgt atgttttatt taaactgtct
    19801 atgaattttt taattaaaca tctggtaacc agctataatc cttttagtag atatctatta
    19861 acatacacct attaattctt taagtgtttt taaaacatag tctaaatctt ccttagtaat
    19921 atctacatga gttacaaatc tatattcacc attttccatt ttattaattt ttatatttct
    19981 ttcaaaaaat tcttttatta aaacatcctc ttttataata tcttcactca tcttaaaaaa
    20041 taccatactt atatctggat taacattgtt tacttcaatg ccatcaattt tatttaattc
    20101 acaagctaaa tattttgcat tttcatggtc ttcattcaat cttaaagtca ttttttcaat
    20161 ggcaattata ccagctgctg caactatacc tacttgtctc attcccccac ccataacttt
    20221 ttgctttctt ctagcttttg ctataaattc tttacttcca gcaagtatag aacccattgg
    20281 tgcagctaaa ccttttgaca cacagaacat aacactatca caacactttg ctatttcttt
    20341 agcttctaca cccaaagtta atgctgcgtt aaataatctt gctccatcta aatgtactgg
    20401 taagttgtgt ttctcagcta tttttttaat ttctcttaaa ttatcaagac tcacaacagt
    20461 tccacctgaa tatgcatttt ctacacatat aagccctgtc tgtggttcat gtatgtcatc
    20521 tcttctaatt gccttttcaa caagttcagg atttagagct ccactttcgt cttcatctat
    20581 agttctaagc tgaactccag ctattacagc aggtgcccca acctcatgag ttacaatgtg
    20641 ataaccttta ccaataatta tttcttgccc tcttaggcaa tgagtaaata gagctaattg
    20701 attcccaaaa gttccacttg gaaggaaaat agcatcttct tttccaactt tagctgctgc
    20761 taattcttca agtttattta cagtagggtc atctctaaag attgaatctc caacaggagc
    20821 ctttgccata gcttctctca tttcgtcagt aggcatagta acagtatcac ttcttaaatc
    20881 gataaatctc attttttcaa ccttctttca tatttatata ctatagaacc ttaaaattga
    20941 ttttttctgt aaagttcata tactttcata gcttatactc tacacaataa ttatactata
    21001 tttactttat atttaatcta ctttaaatta ttaattttaa aaataaagtt tcctctctaa
    21061 taagtataaa taattataca atttaaatat taatctctat cgtttaaatt ccccctagtc
    21121 acaaagcgta tattatgtgt ataattaatt tactgactca ttttggtcaa agaaaggggt
    21181 gctataaatg gcattagatg gattagttat acattcaata gtcgatgaat tatcatcaaa
    21241 acttacaggt ggaaaaatag ataagataca tcaacctgaa gatgatgagg taatttttaa
    21301 tataagaaat aataaagaaa attttagatt ggttttaagt gcaagtgcct caaatcctag
    21361 agtttatctt acaagtaatt atcaaaaaga aaaccctcta aaagcgccta tgttttgtat
    21421 gttacttaga aaatacatac aaggtggaaa tatagtagaa atttctcaaa taggttttga
    21481 aagaataata aaaataagtg ttgaatcttt agatgaatta aaagaaaaga cagttaaaaa
    21541 tattatgatt gaaattatgg gaagacatag caacataata ataactcatg gtgaagaaaa
    21601 taaaattata gattctataa aaagagtccc ttttagcata agtagagttc gtcaagtttt
    21661 acctggtcat gattattcat taccaccaga acaaaacaaa ctcaaccctc ttgatgatat
    21721 atctaaggat ttgtttatta aaaacctaga agaattggaa ggacctatct ttaagtctat
    21781 atattctaaa tttctaggta taagccctat tatagctaaa gaaatttgct acagagcagg
    21841 tgtaaatcaa aatgctatca taaaggatat atctgatgaa caatttgatt ctttacacaa
    21901 agtattttgt aatttattca atgatataaa ttctaacaaa tactcccctt gcattattat
    21961 agataaaaag gtggataagg tagttgattt cagttgtata aaccttacat tatttagcga
    22021 tttatcatat ataaataagg atagtatgtc tcgtattcta gaagatttct atagaactaa
    22081 agatattaaa gacagaatta atcaacgttc atctgattta aagaagagta tatctgttaa
    22141 gcttgataga ctttataata aacttaaaaa acaagaagaa gaattatcag aatccgaaaa
    22201 tgcagacatt tataagataa aaggagaatt gattacttca tatatttata tggtagaaaa
    22261 aggtatggaa agtattgaaa ttgctaactt ctatgatgaa aattgtaatg atgtaacaat
    22321 agaattaaat aaaaacttga caccatctga aaatgctcaa aaatacttta aaaagtataa
    22381 caaaatgaaa catgctaaag tcgagatatc tcatcaaatt tccttaaata aggaagaaat
    22441 agattactta gaaaacatag ttttaagtat tgaaaactgc gaaaatttag ctgaattaca
    22501 agatataaaa gaagaacttg ctaaggttgg atatatcaag actcaaaaga aaaacagtaa
    22561 aaaggatact atcccatcta caaaacctca tgaatttgtg tcttctgatg gatttaaaat
    22621 attggttggt aaaaacaata agcaaaatga ctatcttaca ctaagacttg ctgataatga
    22681 tgacttatgg atgcatacaa agaatattcc tggttctcat gtaataataa agtgtgctgg
    22741 taaggaagtc cctgataata ctgtatttga aggagctatg ctagcagcat tctttagtaa
    22801 gtcaaaactt tcttctcagg tgcctgtaga ctatactaag agaaaaaatg tgaaaaaacc
    22861 tagtggttct aaaccaggta tggttattta tgaaactaac agtactattt atgttactcc
    22921 tgaagaagaa acagtagcca agcttaaggt taaatctgaa taaattctgc actaaataca
    22981 aaataaatgt tagacaagca cacattatca ttctaaataa aaatagtggc cttcttataa
    23041 agtacaataa taaggccact ttctaattag cttactttta attttctaac aaaatacttt
    23101 tctgttttaa taactatact tatattccca acaaatattt tcagtcttct tatatagatg
    23161 taattaaaaa gttaacacta aaagctattt tttaatttag tgatttgttt tagtaattca
    23221 ttatattctg attcatacaa ttcttttttt agattatctt tttctataga taacatagat
    23281 ataacagttg taagtctatt ttctaatact agaagattct cttttttgat ttgcttgtct
    23341 ctactaggag tttttttatt tctgctaatt atatattcgt cataattaca atcaaactcc
    23401 cttattttag catcttttat ctctataata tagttacaaa tatgtgatac aaatactcta
    23461 tcatgagaca ctataagcat agttttatca gtatttatca gtgccttttc cagtgcttcc
    23521 atagatttta tatctaagta atttgttggt tcatcaaata ctaaaaaatt attatcttcc
    23581 aatattattt tacatagtgc tatttttacc ttttctccac cactaagtac ttttactttt
    23641 ttgtagacat catcaccttt aaacccaaat aggcttaagt ttatccttat aaaagattca
    23701 tcaaatgaac tattaacttt agtattgtat agaacactct tttcctcatc taataaactt
    23761 tgattttggt caaaacaacc tacttttacc ttgttattta tttttatatt gtcattttta
    23821 tcagccaata tttcttttat cagagtactt tttccacacc cattatcacc taataatgct
    23881 atctttttac ctctttttat cttaaaggaa acattatcta acaagagctt attttctaac
    23941 tttagagtca tatcttttac ctctaataga tttttgctaa ttatctccat accatcttga
    24001 atcttaatgt tcatttcttt agaaacttta ggtttattct ttacctcaag tttatcaatt
    24061 ctgcttttta tagccttcac atttgagtcc aactttttct tattagtctg accacccatt
    24121 ttgtgcaacc tagcttctga attacccatt ctttttggtg tctttcttat gccatcactt
    24181 aaagcttttt tatataaaag agctttttca agcctttttt tctctttaac atattcttga
    24241 tactcaaaat cagctctttt tacttcttca tctttttgca ttaaataatc agtgtaattg
    24301 cctttgtaag cttttagttt tccatcttct agttctaata tagttgtaca taactcatcc
    24361 agaaaacgtc tatcatgaga tattaataat aatgctccct catgcctttt aaacatatct
    24421 tccaaaagcc caatgctttt tttatctaag tttgaagttg gctcatcagc tattatgata
    24481 ttttttttat tactcagcgc atctgctatt tttaacttaa ccttttcccc tcctgataag
    24541 tattcttcaa atttatctgg agcatttaaa agactcttct ccttactatg actacatgtt
    24601 tcaatattat tttcactctg acttatataa gaatagcttt ctgtaaggta agtatatcct
    24661 tcatcaattt ctatttcacc caatattgct tttaataatg ttgtttttcc ttgaccattt
    24721 gaaccaacta gacctatttt gtcattttct aatatttcaa gtttatctat atccaaaatt
    24781 aatttatctg cataatattt ctttaaattc tctactttta ctaacaacat aaaaaatccc
    24841 tccttaacac acttaaatat aaggagagat tctataaata ggcataacaa ataaacgtat
    24901 agactataat ctactctcct taatttaagc attgcaaata aagccatcaa aataggcttt
    24961 tcaaaaatta caatacttta aattataaaa aatagattat attctcattg tcctagttac
    25021 cttgcccttt ctttcattta tttttaatca ttatatcaac attaattaca aatgtctata
    25081 gaaaattaac taattttagt tattcaatat acaatttatt ctttaaattt attcttcttc
    25141 aactttaaaa tcctgattat ttaaaattct gtttaataca attccaacaa ttgcagctaa
    25201 actaagacca gttatcttta ctgtacttgt tatagggatt ccaatagaaa tgcctttatt
    25261 agataaatat gttgttccaa gacctattaa taatataact gctattatta atatatttat
    25321 cttactttct acacaagaac tgtcccttat agtttttaat cctacataag ttatcattga
    25381 aaatagcatt atactaattc cacccataac tgaacctggt atactttgaa gaaatccacc
    25441 aaactttcct acacatgcaa gcaatatagc aaatattgct gtacgtctaa gtattgaagg
    25501 gtcatagttt ttagttatag caagtactgc tgtattttct ccataagttg tattggctgg
    25561 tcctcctaaa caccctgcaa ctatagtcgc aaatccatcc ccaagtaatg ttctatttaa
    25621 tcctggattt tctataaagt ttttacctac tacagcacca ttagtagtca tatctcctat
    25681 atgttccata aatacagcta atacaactgg agatataatc acaattgctc ctaaactgaa
    25741 cttaggaact gtaaagttcg gtattgcaaa taaacttgct tctgtaatag tagttatatc
    25801 tactaacctt aacagtaaac agattatata cccagaaaaa acggctatta atattcctaa
    25861 ttgttttata aatcctctac caaacttatt ggttaaaatt gctattgcta aagttataaa
    25921 tgctattaca aaattagctg aagccatact aaaagctgtt ggcaataaat ttaatcctat
    25981 aactactatc atagctcctg taacttgtgc aggaaaatat aatttaattt tatttacacc
    26041 aactattttt actataatcg acattataat ataaattaat cctgctacta ttataccacc
    26101 ttgagcatat gctaaatctc caccatacgc ctctttagct gctagtataa ctggtataaa
    26161 cgcaaaactt gaacctaaaa aggcaggtac ttttccttcc gtacaaaaat gaaatatcaa
    26221 tgtaccaaca ccagcacaga aaattgcaac tgacggatta aaacctgtca aaatgggaac
    26281 taatactgtc gctccaaaca tggctaatag atgttgtaaa gacattattg tttttttcat
    26341 aaaaacctcc aaaattatta tatataaata aatcctctgt ttaattaata tcttttaatt
    26401 aattatcatt ccctagattc tattttttaa cctctatata cttattatat aatttatcat
    26461 tattttacat tttacgacat caaaaactca aataaaggag aataaaaact acattaagac
    26521 tcacatccat aaaattataa tatttatttt gtgcaatcta tgtaagcttt atcctcctcc
    26581 ctgactattc aatgctaagc ttttatataa tacttaaaac taattattct ttgattgtaa
    26641 ctgaatcttc tccatcaatt tctaataaac tgacagatat aatttcatgt ctagaagtag
    26701 gtacattttt tcctacataa tctgctctta taggcaactc tctatgccct ctgtccacta
    26761 gaactgctaa ttgtattgat ttaggtcttc ctatatctat aatcgcatct agtgaagctc
    26821 tcacagttct tccagtgtat aaaacatcat caaccaaaac tactgtctta tcatttacat
    26881 taaaatctaa ataagttcct ttaactacag gttcaacatg aattttttcc aaatcatctc
    26941 tatataaagt tatatcaata tctcctgtat ctacttttgt tccttctatt tgttctattt
    27001 tcttagaaat tctatttgca attggcacac ctctagtttt tattccaact aaaactagat
    27061 tttctacacc tttatttctt tcaattattt catgacttat tctagtaatt gctcttgcta
    27121 ttgccttttc atccattaat tgagcttttt ctaccatatc ataattattg tcaaagatat
    27181 aatatatcta tttgacaaat ctactccttt cttttaattt tttataaatt tatttttatt
    27241 taaaatttca atttaaacaa caaaaaactt ctcatcacaa gacaagaagc tcttatataa
    27301 aaaatataca gtgtaattta gcaattgtct tacgatatct ctgtatcgtt taaagataat
    27361 tttttaatct atcaactcaa attatatatt tatatttcaa atttttcaag cttttttaaa
    27421 caaaatactt taatataaga tattttattt aaatttcact aaatttattt acttatataa
    27481 aaacaaaaaa tgatttataa gtattataca aatggttatt ttatcttctt aatagcttcc
    27541 ttaaaatact caggtaattc tgaattaaat tcgatatatt ccttagtggt aggatgtata
    27601 aatcccaact tcttagcgtg caaagtttgt ccttttaatt taaatttagt ttcttttgga
    27661 ccatatacaa aatcacctaa aagtgggtga tttatagaca aagcatgaac tcttatttga
    27721 tgagttctac cagtttcaag cctagctctc atatgtgtaa acttatcaaa tctttctaca
    27781 acttcaaaat gagtaattgc attttttcca tcctttacta cagccatttt taatctatcc
    27841 ttaggatttc tccctatagg tttatcaaca gttattttat cttctttaac cacaccatag
    27901 catataaatt catactctct agttatagta tgttccttta actgttctgc tagaaaatta
    27961 tgtgcataat tgttcttagc tatcataaga agaccagaag tatctttgtc tattctatga
    28021 actataccag gtctaataac tccatttatt gacgacaact tatctttaca atgatataaa
    28081 atcccattta ctagtgtatt ttcatagttt ccaggagcgg gatgaacaac catatcctgg
    28141 ggtttattta ttattaagac atcatcatct tcatatacaa tatctattgg aatatcttga
    28201 ggtaaaacct ctaatacttt aggttctggt atctctataa caatattatc ttcctcttta
    28261 actagatatt tagctttttc tactttatta ttgaccttta cttttccatc ttttataatt
    28321 ttttgtatat aacttcttga catatcacct aattgttctg ctagataaat atcaagtcta
    28381 acttcttctt cctcttcaag tactaaaaac tcttttactt catccattat atcacctact
    28441 tttactttca aaaaataaaa catatataca taaaaacaca gttcctacaa ctacaaatac
    28501 atcagctaca ttgaatacat attcccatat aactctaaag tcgaagtaat ctaccacaaa
    28561 acctaatcgt actctatcaa ttagatttcc taatgcacca gatataatta atataattcc
    28621 caaccttcca aatatatgta ctttccttgt attaagatag tataatccaa atactgttgc
    28681 aagtaatgca acaattataa atatccactg attattttgt aataatccaa atgctgcacc
    28741 tctattttcg acataagtta aatgaaatac attatttatt attggtattg ttgaaacatc
    28801 taccaaatta ttcaataccc atattttaga cagttggtct aagcctatga gtagaattat
    28861 tattaatata tatagcaatg atttttcccc ctataaatta tttaaaaacc aattttattt
    28921 gaaacacact attattatta tacaaaaaaa atgctctaaa gagcaacttt attaaaattg
    28981 tattttctta ataaagctag agcaaaattt aaaatagtac catctttttt atattgcaga
    29041 ttgctacata tttaaaatca tattattatg tataaaaaat ttaaaaataa catactaatt
    29101 aatacttttt gtgtgtttta tagattaaag attaatgatg tctgtacttt aaacttacaa
    29161 gcaccactaa tcttcaaaca aatattttta cttttgcact tgcttatttt tatctaaatg
    29221 ttgtataagt tcagatactg ttacgaattt atatcctctc tcttttaatt taggaattac
    29281 tacttctaaa gcttgtatag tttgtgattt atcatttctt ttagtgttat aatcatgtaa
    29341 aagtattata ttaccatttt ggacctttgt aagaatagta tttataattg tatctacacc
    29401 agggttagac caatctctag ggtctaagtt tgaccaaaga attatattca tgcctttaga
    29461 aactataata tcacagacac ttttactcat ggctctatat ggaggtctaa atatctttgg
    29521 ttctactcct attacacttt ttataatatt ttgtgtatct gttatttctt tttcaacact
    29581 accataacta ttttttgcaa catttatatg tgtaaatgta tgattcccta tttcatgccc
    29641 ctcatcatat tgccttttta ataattcttt gttatattct actttttctc caactacaaa
    29701 aaaagttgct tttacattat ttttctttaa tatatcaagt atttgtggtg taaaatcctc
    29761 atctggacca tcatcaaaag tcaatgctag aatcttttca tcctcatttc ctttttttat
    29821 tattacatct tcataatcgt cttttatatt gtcatttaaa gtataggttt cttttgaact
    29881 attttgatta tgtgtattat ggttatatgt atatacacct actatggata ataaacatat
    29941 acatataccc catattgctt tcttcatgtc attacctctt ttctaattca ttctaacaat
    30001 atatgatgtc aggcttctaa tctctcaatg ttccattttt atttaaatca gttaaatttg
    30061 ttaggtcttc tgaataaaag ttagaatctt ctacatcagg attttgcatt gcatcaccta
    30121 ataaagcatt ttcttttttt gcacaattgt tacataaatt tgtatcaggt aatatatcta
    30181 atctttctaa atctatttga ttgtgacata aatcacaaaa cccatatgtt ccattttcta
    30241 ttttagataa tgctgaatct atttgataca gtcttccttc ttcatgattt attaaactat
    30301 tttgcatatt ttgcatatat aaatccgttc ccatatctcc aatatgattg tcataactag
    30361 ataattctcc tgatgaatat ttttcactag tatgtttagt agtgtctcca aacaaggtat
    30421 tatctttcat atctacgact aaattagtta acttttctct ttcttttaat aatttatctt
    30481 tgtatttttt agtatccatt ttcaacacct ctaattttaa tatttatatc ataaaaatag
    30541 tatgtggaaa actaaattaa aaatacaata aaaaacaaga tttgattttt tcaaaaaaaa
    30601 ttgtaggtaa acttaaaatt cacctacaaa ttctttacat atatttttaa tataatgttt
    30661 ttagtttttg ataataatta cattaacttg ctaatttatg agcattttgc tcttttatat
    30721 ccttagcttt tttaaaaaat aaaagtccaa ggattattgc aactacaata cctattccat
    30781 tagatatatt aactggaagt ctaaatcctt cttttgcctg cattatataa ctaaatgtta
    30841 ctacagtcat aaaaagtgct ggcaatgtag ttatgtaatg atttttattt gctttgacta
    30901 aatatgcaga accagtccac aacattatca tagcaagtgt ttgatttgcc caagagaaat
    30961 atctccaaat tatattaaaa tctataaatg taagggcaac ccctacagca aataatggca
    31021 aagctattaa aaatctattt ttaactgcat cttgattata attcattgca tcagctattg
    31081 ttaatctagc acttctaaat gctgtatccc ctgaagttat aggacatgct acaacaccaa
    31141 gtatggccaa agcgcctcca ataggtccca ttaatccaac agatactgta tttacaacag
    31201 ttgctgctcc accatcatta aatattacat taagctgagg tataccatgg aaatatgaca
    31261 tagctgctgc tgcccaaact aaagctatta taccttctgc aaccattgac ccataaaata
    31321 ctggtcttgc ctcactctct ttctcaacac atctcgccat aagtggtgat tgagttgcat
    31381 ggaaacctga tattgctcca catgctattg ttataaataa gtatgggaat aaaggtgttc
    31441 catttggatt catattttgt aaatttatat ttggtatgtt ataccccttt attattaatc
    31501 ctccagctac acctactgcc ataattaata atgctgctcc aaataaagga tatattttac
    31561 caatcaattt atctattggt aaaacagtag ccataatata ataaagtatt atcactccaa
    31621 cccatatttg tgcgtttaca cctgtcaatg tctttaataa tcctgctgga cctgttacaa
    31681 atacaactcc aaccaatact aaaagaataa cactaaatat tctcattgct atttttgcac
    31741 catttcctag ataaagacct accaactctg atgcagatgt acctttgttc ttcattgata
    31801 aatatcctga agcaaaatcg tgtactccac ctgcaaagat tgttccaaac acaatccaca
    31861 aaaatgctga tggtccaaat aacgcccctt gtatagctcc aaatatcgga cctaaccctg
    31921 ctatatttag aaactgaatt aaaaagattc tcttccattt cattggcaca taatctactc
    31981 catccctaca agctatagct ggagtttggt ttttgtcatt gattccaaaa accttttcta
    32041 caaaagtacc atagataaaa taacctacta taagtactac tattgaacct aaaaagctta
    32101 ccataaattc atccccttag tttttatata attgctttat aaatttttat gagtttaaga
    32161 aaactatttc cagttcaaat agtaatactt ttttatgcta atataaatat taattacatt
    32221 aagtgcatat ttaagtacat gaaatgactt tatatatgca ttaacagcct aaattctttt
    32281 actttgcttc tgcttacagt tacttcatag tcactatttt ttaatttcaa tatgtatgta
    32341 ttgttaaacc atggtataat ttcttttact ttggttaaat ttacgatata agacctatgt
    32401 gttctaaaaa atgttttatc atttataaga ttctctactt ctgatatgcc ttcttttaat
    32461 ataaactttt ctttctcagt atatataaat gtctgccttt cattagcttc acaataatat
    32521 atatcatcaa tatctattac aaccaattta tcacctttcc aaaggctaat tttatgtgtt
    32581 atttcttcta tctcctgatt tactgtctct tttttatatt tatataaatt actattgcta
    32641 ttagacagtt caatatcact catctcactt ttttctaatt tattcaacat agaaataatt
    32701 ctctcttcag aatatggctt cagtacataa tcaaatacat ctaaagaaaa agcatctact
    32761 gcataatttt catacgctgt tataaaaaca attttaggcc ttgatttaaa ctgatttaat
    32821 gtttttgcaa gaagcattcc atcgagatga ggtatattta tatccaaaaa aattacatct
    32881 attttatttt cctgaataaa gtctaacact tctataccat tagtaaattc atttactacc
    32941 tctatgctac ttttattctc aataaaatat cttaattcct ttcttgccgg aaattcatct
    33001 tccactatta tagctctcat acttctactc ctttacataa aatactatca aagtcccatt
    33061 gtctgttctt cttatattaa gccctcttcc atacatcagt ttaagcctta aatgtacatt
    33121 ataaagtcca atcttatttt ctggcatatt atcttgatat acattattta taatttcctc
    33181 acttatacct ataccattat cctctattga tattctcaca gtattttcta atgaataagg
    33241 taactttttt atggatattt tcactacacc agctctacca ctttctaaaa taccatgtat
    33301 tatcgcattt tccactaatg gttgtataat caaacttgga atctttatat ttacatcttc
    33361 tatatcataa gatacagtta gtaattctcc aaacctagct ttttctatct ctacaaaaga
    33421 tttaacttgt tcaatctctt tatttatatc aattaagtta tcactatatt caagattata
    33481 tcttagataa gttgataaat ttattattaa ctctctagcc ttatctggac taaatcttat
    33541 aaatgaagct attgtgttta gggaattaaa caaaaaatgc ggattaattt gtctttgaag
    33601 tgcttttatt tctgctttat ttctcgcttc tatatctttt ttatctttct caatcccctc
    33661 tactatagat ataacaaatc ctattccaat ctgtccaaca attaaaggta aatatatccc
    33721 aacaattatt tgaattgcta aagaatgtgg atatgatatt aagtatataa gtagtatagt
    33781 aaaactttca gaaatcatac ctaccaatat accatacatt actctatgtt gctttggtat
    33841 cctcttatat agaaatcctg ataatacacc tgctagtata cttgataaaa gacatggaac
    33901 agatgttatc cccccaatat ccataaaata tctatgaatc cctgaaaaaa ctcctgctgt
    33961 aatacctacc attggaccaa ataaaatgcc acctgatact atagatatta ttctagtgtt
    34021 tactattgac cccatataat ttataccact ataagttcca aatatggcta aaaaagtaaa
    34081 aactaatgct atacagacca agtcattaaa actatattct tccttttgaa atatctgctt
    34141 aaatattttt aactttgtta ttagaagaaa cagtactaca atgaggcttg tcttttctat
    34201 taatgcgatt aaaattgatg ttttcatatt ccacctttaa aaaataaatt tttacacact
    34261 ctaaatttat tatgtttgtt gtaacttata tatttgaagt aatcctatct cgacattttc
    34321 cacatttaac cttttaatta aataaaaaat ggatatactt ctattaacaa ctaatactag
    34381 ctgactaaca caagcttgta ataacttgta gttatcatta actctacaat tctagtattt
    34441 aattttatac tttagaaatc tcttttctta gaagtaatac tttcgaatat tatataatac
    34501 aatgttatgt agattcttac tcatacaaca tatttcttta aaaattttat actattcttt
    34561 gttcaaatat ctcatttgat ttaattgtgc taaacacaca ttatcatctc tctgttatat
    34621 atttattata tagccgtata tcgataaagt aaataaatcc tatatttaaa atcatatgtt
    34681 tgatagtaaa tcattataat gtatatttac cataataatt tgccaacctt gaaagtcact
    34741 tctataatta gaagttaaat aattcttcta aaaaaaatac ctctaaattt tattttctga
    34801 aatacatagt tataactatt tttatcacgt ataagaataa aaattccag
//
LOCUS       DAMKPG010000033        34769 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMKPG010000033 DAMKPG010000000
VERSION     DAMKPG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.036"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(149..2029)
                     /locus_tag="PC621_001727"
     CDS             complement(149..2029)
                     /locus_tag="PC621_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF4108887.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(2239..8202)
                     /locus_tag="PC621_001728"
     CDS             complement(2239..8202)
                     /locus_tag="PC621_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HDF4108888.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8326..8411)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8583..9986)
                     /locus_tag="PC621_001729"
     CDS             complement(8583..9986)
                     /locus_tag="PC621_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF4108889.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10448..12343)
                     /locus_tag="PC621_001730"
     CDS             complement(10448..12343)
                     /locus_tag="PC621_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF4108890.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(12969..13112)
                     /gene="scfA"
                     /locus_tag="PC621_001731"
     CDS             complement(12969..13112)
                     /gene="scfA"
                     /locus_tag="PC621_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF4108891.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(13178..13540)
                     /locus_tag="PC621_001732"
     CDS             complement(13178..13540)
                     /locus_tag="PC621_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF4108892.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(13598..13891)
                     /gene="yajC"
                     /locus_tag="PC621_001733"
     CDS             complement(13598..13891)
                     /gene="yajC"
                     /locus_tag="PC621_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF4108893.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(14025..15146)
                     /gene="tgt"
                     /locus_tag="PC621_001734"
     CDS             complement(14025..15146)
                     /gene="tgt"
                     /locus_tag="PC621_001734"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF4108894.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(15225..15710)
                     /locus_tag="PC621_001735"
     CDS             complement(15225..15710)
                     /locus_tag="PC621_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108895.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(15722..16747)
                     /gene="queA"
                     /locus_tag="PC621_001736"
     CDS             complement(15722..16747)
                     /gene="queA"
                     /locus_tag="PC621_001736"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF4108896.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(17065..18084)
                     /gene="ruvB"
                     /locus_tag="PC621_001737"
     CDS             complement(17065..18084)
                     /gene="ruvB"
                     /locus_tag="PC621_001737"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF4108897.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(18191..18793)
                     /gene="ruvA"
                     /locus_tag="PC621_001738"
     CDS             complement(18191..18793)
                     /gene="ruvA"
                     /locus_tag="PC621_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF4108898.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(18786..19295)
                     /gene="ruvC"
                     /locus_tag="PC621_001739"
     CDS             complement(18786..19295)
                     /gene="ruvC"
                     /locus_tag="PC621_001739"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF4108899.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(19607..20236)
                     /locus_tag="PC621_001740"
     CDS             complement(19607..20236)
                     /locus_tag="PC621_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108900.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            20439..20762
                     /locus_tag="PC621_001741"
     CDS             20439..20762
                     /locus_tag="PC621_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF4108901.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21030..21884
                     /locus_tag="PC621_001742"
     CDS             21030..21884
                     /locus_tag="PC621_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108902.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(22070..23506)
                     /locus_tag="PC621_001743"
     CDS             complement(22070..23506)
                     /locus_tag="PC621_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF4108903.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(23577..24959)
                     /locus_tag="PC621_001744"
     CDS             complement(23577..24959)
                     /locus_tag="PC621_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF4108904.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(25095..26000)
                     /gene="garR"
                     /locus_tag="PC621_001745"
     CDS             complement(25095..26000)
                     /gene="garR"
                     /locus_tag="PC621_001745"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF4108905.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            26396..27061
                     /locus_tag="PC621_001746"
     CDS             26396..27061
                     /locus_tag="PC621_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4108906.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            27169..27717
                     /locus_tag="PC621_001747"
     CDS             27169..27717
                     /locus_tag="PC621_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108907.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            27979..28287
                     /locus_tag="PC621_001748"
     CDS             27979..28287
                     /locus_tag="PC621_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108908.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(28449..30239)
                     /locus_tag="PC621_001749"
     CDS             complement(28449..30239)
                     /locus_tag="PC621_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108909.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(30239..31966)
                     /locus_tag="PC621_001750"
     CDS             complement(30239..31966)
                     /locus_tag="PC621_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108910.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(32211..32775)
                     /locus_tag="PC621_001751"
                     /pseudo
     CDS             complement(32211..32775)
                     /locus_tag="PC621_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            33064..34176
                     /locus_tag="PC621_001752"
     CDS             33064..34176
                     /locus_tag="PC621_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF4108911.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(34359..>34769)
                     /locus_tag="PC621_001753"
     CDS             complement(34359..>34769)
                     /locus_tag="PC621_001753"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF4108912.1"
                     /translation="NVAKDLFNGEIVAYEISTKMDTKLVNKTVNQLINMNLAKDCILH
                     TDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPK
                     EMISLINDYIYFYNNERIQLKNGMSPIEYRTHSA"
BASE COUNT        10889 a         6080 c         4024 g        13776 t
ORIGIN      
        1 tttaaaatta aacttaatat agctcaaatt attatacatt ttaacctcaa tattattaaa
       61 ttgttaagat aatgaaaata aaataagaat gcatctaaat atataacttt atatctaaat
      121 acattcttat tttcgattta cttaatattt atttttcaat taaactctta atcttatcta
      181 ctacagatga ggctattcct ccacctactt caaacaactt tgatgaattt ttacctttta
      241 atgatgattc ttgtgattta tctaacgtat ttgtagcaag tataactggt gaattgtttt
      301 tagctgctaa tggtccaact gctaaagcat ctataagttt atcttgattt gcaactacaa
      361 ctccatctag tttagattga gagtagaatt tatctattac tttggcattc gtctcttgtc
      421 tagtttgacc tgccacccta ttgtttgata tatctttgtt tgctattttt ccaacttgtt
      481 caatagcctt gttagatatt ttttctattc caccaataaa gtatatattt gacaccttat
      541 ttgatttaat aaattgtaca gcttcattat ctacaccatc tttttgtgta agtactattg
      601 gagttttttc tttaccggcc agtgatgcta ttgataatga atctgcttct ccatttcctg
      661 cccctatata caatttgctt acattattcg tcttatctat ttcttttgct atattaatag
      721 aagtttgata tcttgtatct ccacctattc tctgtactga tatttttgaa tttacagctt
      781 tgatagcttc aacaactgaa tttggcattg agttgtttcc acctatcaca attacttttg
      841 atggatttag ccttttcaat tcttctacag ttttttgagg aagcttttca tttgatgata
      901 aaagaatcgg tgagtttttt acactagcaa gtggtgtagc tgtaagccca tccaccatgc
      961 ttttatcttc tccatttact attacaactg tatctgattt tgtccaacca ctttgagata
     1021 ttttggtagc tgtctcaaat ctatcagcac cttttaattt tttgactgtg acattagatg
     1081 tagaattact tgaacttcct cctccagaac ttccaccact agacgagccg cctgaattac
     1141 ttccaccacc attatttcca ccattgccat tatctggtgg taattcatcc ggttttgata
     1201 attcattttc aactatgtat tctaaacttt ttggaagtgt aacttcaaca tatttattta
     1261 ttgtagcttt tcttccctta gttatttttt gtgctaatcc aagttttaat attacatttg
     1321 aaccactttc ttgtgtttct ttactaacaa tagaatagtt tatcctaata ttgtttaata
     1381 aatattcaag tgcttcactt ttctctacct tatacttctc agggtcattt ttactagaac
     1441 cagttccacc aattggtttt acttcactct cattaataag ggtaaatatt aatccttcat
     1501 cagagtttat attttcataa tccactgttt tgttgttata tgttacaatt tttaaagctg
     1561 gtttatcgct tgttcctttt cctaaaactt tatagtcagc atttgagtta ctgccagatt
     1621 tttttacaga tattcttcct atattcaatc tatttccgtc tagtaaatct tcatttgatg
     1681 taacatatat atctaatgta gttgtattac ttgatttgtt gtattctact ctacaagtag
     1741 catttgcatt tccacttttt atctgagatt ttatattatc agaccaagta atcttttctt
     1801 tctgctcatt atctactact tcaacctcac cttgtacttt tatactggct tgaaatgaat
     1861 atactatgtt ttctactgag tccacagata tatctatagt atttactttc tcatacgttt
     1921 ttgttccttg taaaactgga gctgcatatg cacatgtaac actaccagtt acagctactg
     1981 ccatagttga tactgctata gttcttataa aagattttct tttactcatt aacacccgtc
     2041 cttatctatc attaactaag tggataaatt tacttatcca cctagctttt atatttaatt
     2101 tataaattac cctaaactta tagttacatt tatatacttt gcttttaata tctactattt
     2161 taaaactatt ctagtaccta atcatattag atatacaatg ttagtattag tatttttacg
     2221 tttttagagt tccacctact atttatctct aacaatatta ccctttactt tattgcttac
     2281 agttataacc ttcttattac tgacatcaat agcatttctt gtagcttttc tagctttact
     2341 attactatca gtaagactta ttgtctttaa ctcatcataa ttcttagctc ctacaggtac
     2401 atacattgta tcacttacaa atctttgacc tactggcgta tctccttgaa ggtcattgta
     2461 tctatatagt tcagccttaa ccttatcact taaagaatca aagttttctt ttggcatata
     2521 gtaaacccat gtacctttac tgatttcacc taattcacct ttttctggaa cttctgcaaa
     2581 taaaagtgct tctccagata tagtcttatt gtttttatct ataagtagtg gtaaattaaa
     2641 tgctggattt ccactgtatt cacctgtaat aattattgaa cctgctggaa tcttacctgt
     2701 ttcatcttca cttgtgctag ggtcatcact ttgatataca taatcttctg ctaactttcc
     2761 aattccatta gtacgagttt ggtctgcatt attcattgtg ccaatttcta tattatcacc
     2821 tggaggtcct attatatcta attctgcaac ttctatatta gacttattgt ttgagattag
     2881 ttttacatat ctagcttggt atgtccaaag tttatcatct ttattaaatg taactctagc
     2941 ttcattatct tggtcttttc ctccaccaac taaatcttgt ccatattcaa aagttccttg
     3001 acttacatct tcccagttct ttttatcatt acttacttgt atcttgtaat tagttaaagg
     3061 attgtatgtt gtgctagtat tctttctaaa gttaaataat ccttttaatg agaacttcct
     3121 agtttctgcc tctggttttg tatattttag tcctactata ggtaatgttt ctttcatgtc
     3181 tactattata tatggatttt cttctacatt tatatcaggg tctttttgag ggtctttgtt
     3241 ccactctgct ttacttatta tctttccttt atatgtagta cttacatcat tatcttttat
     3301 attatcaatt tcaggatttt gtaatggtcc atgtgagtca ttttcagttc taacatcacc
     3361 gtcagaaaca gtatttgtta tgaatgacca tgctgattta tttattttcc cttcatctga
     3421 taattttaca gtgccaactt caaattcttc tgttggattt aggtgataat catatgcaac
     3481 taccttatat gtaaatgctc tgttattaag aggagatata ctatcagtaa atttggcctt
     3541 actatttcct tttgcatcca caaatccaac tgttctgtat tttacctgtg ttccattttc
     3601 accatctaaa taatttccat cacttcttat tatttcgtat cctagtatct tatctttatc
     3661 cttatctaca tctaggtcta tagatatttt attagattta actagagaac cactttgtat
     3721 tccatcagaa aaactagcac ttactttagt atcttttgcc atactagcta tattttcatt
     3781 taaaatcttt cttctagctt cgtcatttac atattgtatc tttcttgatt ctttttcata
     3841 gttcttacta tccattatac tgtttaattc tggtgtaatt tctactcccc atgccttaaa
     3901 gtaatctctt aaatcttttt caacaacact tgatgccatt gctactagca tttggtcttt
     3961 tggtaaacta ttaattgggt cactacttga aagtgctctg tattttcttg aaatctttgc
     4021 atagaatgta tcattactta aatcagcatc tgagttattc tttaacattg aacccgtaaa
     4081 atcatcatca taagctagat gtaattgcca taacattcca agcctttcaa atacattgct
     4141 tggtatacct aagctatttg aagttacatg ctcatatatc ttatccattg ttgaaccttc
     4201 aagtcttgaa tgtgtcttat catcaaaggt ctgagcaagt aaagcaagta cattgttact
     4261 agtttcgcta tatgtcatct ttccaatatc tgtaacatgt cctatttcat gacctattcc
     4321 ccatccaaat aagtttcctc ctgtagcttt tccactatca tctatttgga aaggtactcc
     4381 attcataagc cctggaactg aaccatattc tactcctaca tgtaatccag aagcatacat
     4441 aaatgctcca ataaacattc tttggtattt tatgttcata cgagattttg gagccatatc
     4501 atttttagtt aagttaaatt cattatcatc tacagttcca ttaccatcta aatcttgtgt
     4561 tttaaatact cctctttctg agtaaactaa atccattatt tgttcccatg caagcatact
     4621 attatataat ctatctactt gtgtatttaa atcagcattt ccttttgata ttccttgata
     4681 tacagctgtt gcaggaacat ttaatgttac tttatcactt tcaatatctg tagtgtttaa
     4741 cacaccagtt ttttctgaat attcatatga attaccaata tttgttcctg gataatatgg
     4801 ttctcctttt acagcatctt tatatttagc taattcttct atatatgctt ttatcttttt
     4861 cttagattca gaaactcccg caggactctt atcatttatt gttccattta gattaagatg
     4921 tggtatctta gtagctcctg ctacacgtac ttttatatct cctccacttg gagtatcttc
     4981 agggtatctt atgtataaac ttcctccaca ttctatatct aaactatgaa gtttgcttaa
     5041 ttgaattaca ttttttccag ttttaagatt tatttctcca ctcttatatg caccagattc
     5101 tccatagtac tgtgtatata tcaacttagg agtttttcca cttttacttc caacatatac
     5161 tgttatttca tctcctgctc tagcagctac tcctaatgcc tgccagtcat tttgcatgcc
     5221 taagttattg gaatttacag tatactgtcc gtttttatct ttatattttc ctgatttatt
     5281 tatttcttga tttacagtag ttacatcagt aagtgtggct ctatcattat ataagtcttg
     5341 agctaatttt aattctctat caataactgt cttatatgga tgatattctc cactagcttt
     5401 atctggagta tttaatctat cttttaattc agttattttt tcttgagtta catcttcttt
     5461 taattcaact tgtaggtcat ccataaatag atttcttaca tcatcttcta tactgtcata
     5521 ctcataaaac ttaagttctg atatattaga cttatatgaa taagatgaac aaacacttac
     5581 ttgtatctta gtagcagtta ttggttcttt taatctaacc attacatact tattattatt
     5641 tacagggtct gtctttgttt caaaaggaac atcccccaga ttgtgtaatt gaccattttc
     5701 atcccaatat cgtatctcaa agtcatttat tgtcttatca gaaacatcaa aacgaggtgt
     5761 agccataaaa gtatccatag tataagattt atcaaaagtt attattggtc ctctattcgt
     5821 tccctcagct gcattatatg ttgggaatgt ccaatgtgtt gtgaagtctc catctactac
     5881 attgaattta tcaaatggat ttttatcact atattcactt ttgtcatatc ctgctggata
     5941 aacaacatct acaatgtgat ttgttggtat tccaaatcca ccattctcct taggagtatt
     6001 aattaatcta tagttaggtg ttgttggtgc atctatgctt tcagttgtac ctatatatgt
     6061 tttagacatt ccactagttc ccaagtggtt agtagctgtc attctaactt catatgttgt
     6121 gttatcctta agaccttcta tttttaaact tgtcccacga ataagcttat cattgtattt
     6181 tatccaagac ccattagagc ctttttcttt atagtacaag tcaaagtctt tagcttgttt
     6241 atgatttttc cagcttacat caagagattt aaatttacct ttaacattta taccttctgg
     6301 tggttctggt tttgtgtctg gaacaacttg aaattctaat atgctatctg ggctaaattg
     6361 tataggttta tagtttgagt ctacattttc tggtactcca tctaagtttt ctggcgttgc
     6421 atctttataa ccactagacc aatctccatt taaagattgt atgctaaccc tatatagtcc
     6481 atatgcttct atatctctaa cttctattgt atttttacta gtttgtaatt ttttagtaga
     6541 taatactttt cccttactat caattttatc tagtataagt tcgtatgctg atacatttac
     6601 ttcattattc caataaaaag atactttttc atttccaaca gcagtgctag tcgatatttt
     6661 atttatttta ggtatgttca tctctggctt tggtatttct ttatatacat tgtttaaaaa
     6721 ttcaactttt gatatttctg caagatttct atcgtttgct gttgtagctg ttatttttat
     6781 agttatcttt tttactgcta cttgtttacc aagatttatt actatatcgc tattttgaac
     6841 atctgtatat gtttctaatg ctgccctact atatgtagat gttgtagagt tatcatcttt
     6901 actgatatta tccccttctt ttggagtatc ttcatctgaa ttaggtttat ccattgatga
     6961 attttcttta tcattatttt tatcttgatt ggtattagag tttacttctt gtttatcgct
     7021 attttcctgt tgtttatcat tattttcatc tttattattt gaattattat cagtctgatt
     7081 tttattttca tctttattat ttacttcttc ttgatttact tcttcttgtt cgttatttac
     7141 ttcctcttgt tgctccttat cttttaagtt tttcatatat tctttatttt cataaggaat
     7201 ctgttgaaca ttaccatctt cgtcagtaac ctcaactatt ccttttgctg gagcagtacc
     7261 ttctggagct tttatattga tgacttccat attaagtgct tctgataaag ctatatccat
     7321 tactgctggt ttttcctcag atatttcatc attttttgaa gatattgtaa ctccattatt
     7381 ttctccacta aataaatttt ctacattacc attaatatct acatcactat ttccttttaa
     7441 ttcaacctta cttgtatcta ctataggatt ggtagatact atactttcta ggctttttgc
     7501 atcttcactt tgtcctaaat ttttctgtac ataatctaag tcaacaatat ctacctttcc
     7561 atctctattt aaatcaaatt ctcttttatt tgaatctata ttctctaaaa ccttattata
     7621 atcactctta tctactttac cgtctccatt tacatcaccc attaagaata attcataatt
     7681 taaaggttta tttttattat ctacacttgc tctattgctt aataagactc tttgtgaata
     7741 acttgatata tcaatgtctt taatttcttt gttcttaaat ccagctccag agacttctat
     7801 attgtatttt ccaacaggta aattattaaa tacaatatta taatatgata cattctcatc
     7861 tttatttttt atttcacttc tacttgcaga taattttttt atagtatatt ctatattatc
     7921 tgtcccaata tttactgttc ctgaatcttt ttctccaagc tttattattc cactattgtt
     7981 actatcatct tttaatcgta actgcatatt agtcatactt tctgactcgc tattttttat
     8041 tggcagtgaa aaatttaaat ctacttcaag attccccctt aacttttctg taactgattt
     8101 ttcaactgcc cttagcttcc cctctaatat aggttctgca aaaacattta gtggtgcact
     8161 acacatagat actgctagta cacaagatat tgcctttttc atgaaacaac aatcctccct
     8221 taataaatct ctaatttttc atgaatttaa atactataag tttcagacaa taaaaaaaga
     8281 cactcaaaga gaatcttaat tataaaataa aaagacttta ctctttttag caactggctg
     8341 atatggcttt aaaagcttta atccctatag ttttgcgtca ttagatttcc ctaattttgc
     8401 catttaattt tcactacttt cttacccatt ttatactata tttaaacttt ttaatacaat
     8461 ttttttacat taaattataa aaaaataaca tttatattaa taaattaata taaatgttat
     8521 tttccattat aatttaatta tttatatagc ttccttacaa gcataatttt gtgtatattt
     8581 tcttattctt tatatcttcc ataaaattca tgtgtagcat atagattctt atctcctgtt
     8641 atagctatac caaatcccat tatttcatac cctttatgca acatattagt tccttcttct
     8701 ggatttgtct tccactcatt aaaaataaag tttcctatat catctgcatc tctatttgta
     8761 tatacaggtt tatctgaaat tttatattta attatatttt caccttgaac tgctatatat
     8821 ccaggtttaa tctcactcca atctaagtat ttcaaaaaag tagaatatga attttttcca
     8881 tttatcgtat gagataaagt cttcttattt gccattaaat tagaccaatc atttgctaga
     8941 ccctctaatc tgcttgctac agataacgct cttactccat tatcttttct atactgcttt
     9001 gtaagagcat atactttacc ctttattctc tctttaaatg ctctatcatt tggattgtaa
     9061 tataaccctt gttgatttgg tgaagcttga tttatacatt gcttgataac attctgattt
     9121 atgcctccta gtacagtcaa tttgtctgca ccacttaaaa tactcttgtt actgccattt
     9181 tcaactaaaa ctacaggagc attttttgct attgtagagg cgaccaaagc gtctgttaat
     9241 tgcaagcctt tacttagata aaattcttta ctatttttat aaaactcttg tataactatc
     9301 ttattagttt caaatctatc tgtgccaccc aatctaatag aatttgtgct cttaactaat
     9361 tcgtcgctga ttatttcact agaaccaagc gcataactct ttaagcctgt cgtgtcaaat
     9421 cccacactat ttccatctat aagtattata ggtactccat ctctagcagc tactggtgat
     9481 gcacttatcg catctgcttc tcctgtaaat ccattggcta taattacttt atctattggc
     9541 ttaatttctt tgattttttc agctattttt atacttgtat caaatctatt ttcaccaaat
     9601 attctttgta caaccttctt tttggataat aactgattct caacattttt acttattgtt
     9661 gattctgagc ctattatata cacagtcttt gctttgttta atctttccat agttgtgttt
     9721 ggaatctgat tttgctttgt cattaaaata ggcgcattta aggctccagc taaaccactt
     9781 gcacttagac catctgctaa gctattatct gagttgatta gtattgctgt attgtagttt
     9841 tgcttatctg ctatttttgc tgctgtctca tatctgtcat tcccttttat ttgctcaact
     9901 gatgtcaacg catttaccat aggagtattt accattacta aaaatagtgt aagtcctata
     9961 gctaatatct ttttactatt cttcatatta ttttctaccc ccaatgtact tttcatattt
    10021 tttattagta ctcatactta tttttaatca aaagttacaa ttttgttacc atattttata
    10081 tatgatacca tagttatcca tacaactcaa acaatatacc taaagttttt attctctata
    10141 aattcaaaac attataattt attttattta attaagttat aagttcaaaa taatttatgt
    10201 tagtaatatg tttaatcatt ataagaagta tctttttata ataatttttg caatatgaat
    10261 ttctccacat tataatcaaa aaataaaata aggtatctca atctaattct aaatgagaca
    10321 ccttattttt attattttat cttgttattt gtatatttta taatatctac actatgtctt
    10381 acatattaac gcattgtata atcatatatt atctaaataa acttttaata ttaattccta
    10441 tatcttatta gttcttagta aataagttta ttttttctgc tattttttca atagcgtttt
    10501 tagctattcc ttcacctatt tgtactaact tagtagcatt cttaagtctt aactcaacta
    10561 cttcttcttg tgatttagaa agttcttctg tagttaatac tattggagcc tttgaactag
    10621 ctgcaagtgg tgctgctgta agtgcatcaa ctaattgtcc atttccacct acataaccat
    10681 cttttgctac atatgcttta gttgcacctg catagtattt attgattact ttagcattag
    10741 tatcttttct attttctcca gctaatcttt caactttatt atttaaatct atatcaacta
    10801 atttgtcttc aacttctttt gatacagaat tttctccacc cactatttct aattcttttc
    10861 cttttaataa tttttcagca tttgcatcta attcgttacc tgtaactatt attggagcac
    10921 caaattgagc agctctagct gatatactca tagcatcagc ttctccattt ccacctacta
    10981 caaaagcagt cttaaatgta tcttttaatt gttttgctat ttctaaagaa gtttcaaatc
    11041 tatcttctcc agaaactctc tcaactttac ttacacctaa tttagaaagt ttttcttcag
    11101 tttcttttga tactttagat tctccaccta ctatatatat tttcttagaa gatagattac
    11161 ttccaagtac tcttaatatt tcagcttcta tttctgatgg tagtgaatct tttttagata
    11221 ataatattgg tgcatttttt tgagaggcaa gtggtgctga tgctaatcca tcaactatag
    11281 catcttctcc aactaaaatt atagcttctg cttctccatc tttgaatctc tctttactaa
    11341 cttctactgc tgttgtaaat cttgaatcac cagataaaac ctctactttt aattcttttc
    11401 catcttttac atcagttaat actttgtgta agtctctaac taacttatgg tttttacttg
    11461 aaacacttat tgtttctgac ttttcatttg ccttgcttaa aactatatca aagcttgatt
    11521 tttctggttt atctttatca tcactatctt cttgttcttg agtaaggatt gcttcatttt
    11581 caaatgttaa agcatatcca tttttaactt tattaactat ttctcttcct aatgtagtta
    11641 atctaattcc atcaaataat tcttctgctg atatgtcttg taaatctgcg tttttaactg
    11701 ttatcgtatg taattctttt gaatctatgt cactataact cttttcataa ccttcaaaag
    11761 taccttcttc tgatgttatc acttttgaga aatctaaatg ttggtctcct acatttaatg
    11821 taataacatt tgctgaatct tttgctcttt ttacttcaac tgtatttgta gattttatag
    11881 ttgctgttaa cttgttatca gaatcttctg ctaaagttgc attgtaagca ttaactgcat
    11941 ctactatttc ttgagcagtt ttgtagtttt caattttata gtcaactact tttccaccta
    12001 acacttgatg tcctttatct tgaatagtaa cttttaaact ttgcccttct gttaaagagt
    12061 ttattttatt ttgtaattga gttgcattag ttaagtttac attgtctact tttatatcgt
    12121 aaactctatc atttacattt gctcctgctt ttgtatcttc aaacttaact tctaatgctt
    12181 ttctaacctc ttcgattagt ttagcagaat ctttcgcact tactgtgtaa tttttatcaa
    12241 cattttctgt cacagtattg tctgcgaata ttggtgctgc tgcactcata actgtagcac
    12301 ctgccattac tattgccata cttctctttt tgttgcttaa catatttatg tcctccgaat
    12361 attatttttt attttttata ttcgtatttc ccacacataa ataatattat atataatatt
    12421 atttttcaat atattttatg tgtttacata atttactaac acaaacacta ataatatctt
    12481 cttgaacccc ttccccctac ttagctctct ataattatgc tcaaagatag aaatccctat
    12541 tttatagtga ttttctacta aacaatcata tactaacaaa agtattcaaa ctccattttt
    12601 atatactact atgcactaat ttttgctttg ctcttattac ttaaatatat atcactaaat
    12661 taatatctac ctccatatat taaaatatat aacatattat aaaatcatca taaattagag
    12721 tttaaaatta aaaattattc taatacatgt aattgataaa aaataaaata taagagtagc
    12781 tactttaaaa taaatgctct tatatgatta gatatttttg cataattaaa cgataagaaa
    12841 tcaaatttaa ttttacataa gtagaatata tatttttata taataattga aaataaaaga
    12901 ggatacctca taatgataaa agaatcactt tgagataccc tcttacaaac aactaaatca
    12961 ataattttct atctttcaca ctcttgattt gcaactgtac aagaagtttt acaagctgat
    13021 tgacaagaag tttgacactc tccacatcca ccttttgcag cactttgctt taaagtagct
    13081 tgagataaag tttttacatg tttattttcc attttcaacg cctccatttc taattgtttc
    13141 ctattgatta tatcataaaa ctactttaat ataaactcta tttaaagttg acacccaata
    13201 cgcctccaat gcctcctgct gctgatatta gcaataactt ataaatcatc cctacttcaa
    13261 atacaaactt ttcttgtgct aaaaacacag tcaaaaaaat acatactatg tacaaaagtc
    13321 ctactaaaag tccatacata agtccttttt ctttcatgtg ttttgaagtg taaaaccctc
    13381 ctatagctgc agaaatagta gttataaatg atgagaccat agttatatta tctccaccaa
    13441 tatctgtaaa tgtcaaaaat aagttataca ctaataaaac agccaaagtt attatatatg
    13501 catatcctag acctttaaaa atataattag attttcccat aaaaaaacct cctctcaaat
    13561 taatatatat tcgagaaaag gcttttaaat tactaattta gtcttcttct atttcttctt
    13621 ttttagattt aacagttcct atagcccatt tttcaaatgg aactttagtt ctatttggtc
    13681 ctaattctaa tattacaaca tcatcttcaa ctttagctac attagctact attccaccta
    13741 ttgtaatgac cttgtctcct acacttaggc tacttctcat ttcttttaat tgtttgtctt
    13801 ttttcttttg aggtctaatc attaggaaat aaaagattgc gattactact acccatagac
    13861 ctatactcat aattatttgt tgctgtggca ttaatttttc actcctttgt aaattatact
    13921 cttttgtaaa tcataataaa tgtttatata acaatttact ttctctatct ttgagtacta
    13981 ttgaatatta tatattaaaa tgattataat taatagtgca aaacctatat ttcatatcca
    14041 tattttgcaa aaaattcatt tctaaaatca agtaatctat cttccattat cgcttgtctt
    14101 atttgtttca ttaaatttaa taaaaagtga agattatgag tagttattaa tcttgcaccc
    14161 agtatttcat ttgcttttat aaggtgtctt atataagctc ttgaatagtt tttacaagca
    14221 taacaatcac attcagggtc aagtggagta aagtcttcag cataagttgc atttctaact
    14281 actactttac cttggctagt cattgcagtt ccatttctag cgatacgagt aggtaggaca
    14341 caatcaaaca tgtctactcc tcttataaca ccttccacta agtcatctgg actaccaact
    14401 cccattaggt atcttggttt atcctgtggc ataagtggag ttgtatgctc tagcacgtca
    14461 tacataagtg gttttggttc tccaacacta agcccaccaa tagcatatcc tggtaaatca
    14521 atactagtta tttcttttgc tgattgttct cttaagtctt tatacatccc accttgtatt
    14581 ataccaaata aagcttgttt gtcggtatta ttatgggcat ctttacatct ttttaaccat
    14641 cttgttgttc tttctaaaga attttttaca tattctctat ctgatgggta tggtgcacac
    14701 tcatcaaacg ccatcattat gtctgaaccc aatgcatttt gtatttccat agctttttct
    14761 ggagttagaa aatgttttga accatctaag tgtgacctaa attctacacc ttcttctttt
    14821 atctttctta aaggccctaa gctaaatact tggaaccctc cactatctgt aagtattggt
    14881 ttatcccagt tcataaactt atgaagacca ccagctcttt ttataagttc atgacctggt
    14941 ctcatatata aatggtatgt gttactaagt attatttgtg aaccaatctc ttttaattct
    15001 tctggtgtca tagattttac agttgcttga gttccaactg gcatgaatat tggtgtttca
    15061 ataatcccat gtggagtgtg taatcttcct aaccttgcac cactctgctt acaagtcttt
    15121 atcaattcat atcttactgc gtacataaag ttctccttaa ccatatttat ttctcattat
    15181 ttcttaagat atatttgtaa taagctattc atataattta ttgtctaaat agatttatct
    15241 tcgtcttctt tgttctgttg ctccttaatt gcttcttcat catgaatact cattatatta
    15301 agagcactct tgtcttttgt tactaaaaac ttttttatag caaatagtcc tgctatagct
    15361 ataactccta ttaaaacatc tatcatacct tcatttttag gcagtagcaa catttttcta
    15421 gctattgcgt aaagtaatgc ctcaataaat gctcctggta tatgaagaga tagcattact
    15481 acaagttcaa cacctattac taaaagtaat atttgtgcta aaaaatcatt aagctgactg
    15541 tattgtactg gattttgaaa atcaactata tatgctcccc acataagtct tagaacatct
    15601 atagttccta aaaatactgc tattagaact actactgcta aaacacgttc aaatataaaa
    15661 gcaatcttta atatagggtt attctttttt tgtttttgta aactatgcat tcattacctc
    15721 cctactttat aatcatagca tctccaaaac tgaagaatct atatctttct tttactgcct
    15781 cattatatgc atttaaaaca ttatcctttc cacaaatagc acttactaac attattagtg
    15841 tagattctgg taaatgaaaa ttagtaataa gtttatctaa aactttaaat ttataacctg
    15901 gatatataaa tatttctgtc catccactag tttctttcat ctttccatcc tcattacaag
    15961 ctgattctat agttctacaa ctagtagtac caacacatat tacattgttt ccattttcct
    16021 tcgctctatt tattttgtca gctgcttctt ggctaaccat ataatattct gaatgcatct
    16081 tgtgatttaa aacatcttct acttttactg gtctaaaagt gcctaaacca acgtgtagag
    16141 ttacaaaagc tatatctaca cctttttctt ttattttatt taacaattct tctgtaaaat
    16201 gtaatcctgc tgtaggtgct gctgctgaac cattatgctt tgaataaaca gtctggtatc
    16261 tttctttttc atctagtcta gcagtgatat atggaggtag tggcatattt ccaagttcat
    16321 ccaatatttc ttcaaaaatt ccatcataaa ggaattttat aattcttgaa ccttcatctg
    16381 ataaatctac aacttctcca attagctttc catttccaaa agaaaattta gtccctactt
    16441 ttgctctttt tcctggtttt actaaagcct gccaagtatc ttcctctgtt ctttttaata
    16501 ataaaaactc tattttacct ccagtttcaa gcttctctcc tattaatcta gctggtataa
    16561 ctctagtatt atttaatact aaacaatctc ctggatttaa atattctatt atatctctaa
    16621 atactttatg ttcaatatta cctgtctctt tatctaatac catcaatctt gagctagctc
    16681 tatcttctat aggaacttga gctataagtt cctgtggtaa gtcaaattta aaatcacttg
    16741 ttttcaatgt taaacttcct ctcttaaaat ttctccagct acaataatat caaaaaaaca
    16801 acttattttc cacaatatta ccataatcta tcaagaaatt gctattttta acagacacca
    16861 tatccacaat atatagatat ttttccacga tatacatgtc ttatccacaa tatatagata
    16921 caatacacac aatttttatc gtgtttatcc acagtatcta cagatataat taccattttg
    16981 tggatatatt tgaattttta gccattttag aaatatttta atttaatgac tcttttattt
    17041 tataaatact cgtataatct atttttattt ttcattagga tatggaattt ttaagtgttc
    17101 atatgctaat ggcatggcta ctcttcctct aggacctcta tttataaatc ctaattgaag
    17161 caaatatggc tcatatacat cttctattgt atttctatct tctccaatag aagctgccaa
    17221 tgtatctaaa cccactggtc ctcctctaaa tttttctatt atagtcataa gaagcttctc
    17281 atctacaaaa tcgagaccca aactatcgac tcctaaaagt tccagagcat cttttgccac
    17341 cttatcagtt atttttccat cagctctgac ttgtgcaaaa tctctaactc tttttagtaa
    17401 tctatttgct atccttggag tccctcttga acgttttgca agttctaatg ctccattaga
    17461 ctgtatctct gcatctaaga tgcttgaaga cctaagtact atttttgaaa gttcatcaac
    17521 tgtataataa tccaatttac aaataacacc aaatctatct ctaagtggat ttgtaagcat
    17581 accagctctt gtagtagctc caataagtgt aaatttagga aggtcaagtc ttatgcttct
    17641 agcagatggc cctttcccta ttattatatc taggcaaaaa tcctccattg ctggatataa
    17701 aacttcttca acacttctat tgattctatg aatctcatct ataaataaaa catcattttc
    17761 atttaaattc gtaagtatgg ctgccaaatc tccagctctt tctattgcgg gtcctgacgt
    17821 aattcttaaa tttaccccca tctcatttgc tattatactt gctagtgtag tttttccaag
    17881 acctggtggt ccatataaaa gcacatggtc taactgctca tttctcgatt tagctgcttc
    17941 tataaatata ctaagttgtt ctttggattt ttcttgaccc aaataatctt ctaaagtttt
    18001 tggtcttaaa ctgttttcta tatctatatc ttccatcttc attgtagaag ttattatcct
    18061 attttcatct tcaaaacctt gcatttatac ccctcctctc gtacttttga aaatctactt
    18121 tatatatttt caaatttaca taaactcctt aaatattgtt ttaaaatcca aatattataa
    18181 aatccaaata ctatttcaag gatttactca ttatatatgt aagagctttc tttataatag
    18241 cttctgtatt cataccatct tttttacatt tttgaactgc ctcttttgat tccgacaaag
    18301 tatagcctaa agctacaagt gcatctacag actcatcttg tgatatcaag gtaggttttt
    18361 gtgataataa cgttggttca aactctatgt tatttttgtc taccttatcc ttaagctcta
    18421 agacgattct ttctgctgtc tttttaccaa caccaggagc tttggataat tttccaatgt
    18481 cttcacttaa aatatatgct cctaactgtg ctgatgatgc aaatgatagt attgaaagcg
    18541 ccaccttagg tcctatttta gatacagatg ttagtaattc aaacatctta agttcttccc
    18601 tacttacaaa accacaaaga ctcatatcat cttctcttac aattaattta gtatatattt
    18661 ttgtggcttc tcccacttgt aaattcatta ttgtattgga tgatacattt attttatatc
    18721 ctatcccatt attttcaaca actatactgt ctatataaat ttcttctact gtacctttta
    18781 tataactata catacttacc acctatattc tttagagttt tttcaagttt gtttgaatga
    18841 gcatggcaaa tagccacagc taaagcatct gcaacatcat ctggttttgg aacttttttt
    18901 agacttaaaa atgaagttac catctgttga acttgtgcct tatctgctct cccatatcca
    18961 actacacctt gttttacttg aagtggagtg tattcatata caggcttccc attatgagca
    19021 catgcaagca tagtaacacc tctagcttgt gcaactgtta tagctgtttt tacattcttg
    19081 ttaaagaata attcttccat tccaacttca tctatattgt aattctttac tattgtatca
    19141 attcctttat acacaagttc caatcttctc gatatattca tatgggcagg tgttgtaact
    19201 gctccataat cgattacttt aaacttgcta tttttgtatt caattatacc atatccaact
    19261 atggctatac ctgggtcaat ccccaatatt atcatttttt aaaattcccc ctaaaaataa
    19321 aagaaatacc aaaatgtatt tctcataaaa acttatgttc taaatcatta tatcataaat
    19381 aatattaaca aatcataaaa tgctctatgt aaaatataaa atcaaataaa aaagttatat
    19441 caaagcaaga ttttacctca atataacttt tatatttatt acaaataaat tattgtattt
    19501 ttataggaat taaatgtgac tacaatttta atactaatct atttaatatt aaattattta
    19561 ttattaactt acttagtatt taacttgaaa taatattaat ttactattaa cttgcaaagt
    19621 cttctaatct tgaataggcc tcttcttcag tatccataac tacacctttt ttgatttcat
    19681 cttgaactgt tttaggaagt gaatctactc ttatttttgt tttttctact aatgttttac
    19741 ttccactatc atcatattta tatagtgcta tatttccatt atcattttca atagtatatt
    19801 taccttttat agtagcgttc attgcttgta catctttatt atcactatat ttagattcta
    19861 ccaatatgat ttcattttta tccatactct ttattttttt agttggatat tcttttttaa
    19921 aatatgatac tatttctgat tttgtcttat ctaataatga ctctggaact atcccagcca
    19981 ttgtaagtgt tctcttatag ttagtttcat tttcgccttc actttgaagc caaacttcac
    20041 aatttttatt taatccaaaa gcttcttttg acttttcgtc catattcgca ctagcactta
    20101 caactttact agtttgaact ccattatttt ttaatcttgt ccctatataa actccgctac
    20161 ttaatacaaa tactccaacc agtattaagg cagatatttt ccaaggaaat aattcttttt
    20221 tcttcttttc aaacattgat tacgcctcct ttttctatta ttttctccaa gctgtaagtt
    20281 tttatacaat ttaggtaaat tttagaagaa aatatattta tatttcaaat tactctaaga
    20341 atagatattt tctaaaaggt agttttctct taatggttat attcttgtaa ttatatggac
    20401 ataatttata ataaataatc gtttaggagg taataacttt ggaaagaata aactgtaatg
    20461 taattaactg ttcacataac aacagccata tatgttatgc agataaaatt tttataaatg
    20521 gtgaaaaagc tcacaatgac agacatactt gttgtagttc atttttagat gaatctattt
    20581 atagtaattt atctagcaac gttaataata gaaattgtga ggaattagtt tgtactgtaa
    20641 aaaattgtaa atacaacaaa gatggtagct tatgtacact tgataatata ttggttgctc
    20701 ctgaaactga tagagtaaac ctttatattg aaacatgttg ttctagtttc gaatgtaaat
    20761 agtcatagta taaatataag ggtgtcaaat gtaattaaac taacatagtg tagagtatct
    20821 acaactatta gtttgattat acttgacacc cttatttatt ttaaataaaa tttcattatt
    20881 ataaccatgt ttataatgtt ctaactgtaa ttaaatttga taaatttcta taaattggta
    20941 taatcaatat gtaaattgaa tttagcaaat taatcataaa aataaaatat cgagtatata
    21001 gtgttatact aactagaaat gaggtgctta tgagtacatt ttatattcaa tttctttatt
    21061 ttgtaattta tagctttata ggatggtgtt gtgaaactac atattgttca gttttacaaa
    21121 aacaatttgt aaatagaggt tttttaaatg gtccattttg ccctatatat ggatttggag
    21181 cattagcaat agttgctacc ttgactcctt ttgtacacaa tattcctttg ttatttttgt
    21241 ttagtataat tataactagc ataatggagt attgtacgag ctttatttta gaaaaaatat
    21301 ttaatatgac ttggtgggat tattctaaac ataaatttaa tattcatgga agagtttgtc
    21361 tggaaaactc acttatgttt ggtgtattat cattgattgt aatgcttata gtacatccta
    21421 ttgtggttga ctttattaac tctatctcta aaaatgtgct atttatattt gctatatcta
    21481 tagaaattta ttttgtttta gatttagtta tcacagtcca tacaatttta caattaaatg
    21541 gtaagttaaa acaaatcaat cttataatta aggaattaaa agataagaaa gaatactaca
    21601 aacttattac acaagaaact atagaaaaca aacttgaatc tttagttgaa aatagactgg
    21661 atactttgga agatagattt gaaatactta aggaaaaaga aaactatcgc tatgctaaaa
    21721 atcgtattga tgagctaaaa aataaactta atactttgct gtctaaccac aaattacttc
    21781 atagacgtat aatcaaagca ttccccaaca taagctctaa taagcataca gatattttaa
    21841 ataaaatcaa agaaaatatt aaatcaatta aaaaagataa ataataaaat aaacacataa
    21901 gttaaataat aaaaatacta atacataaga ttaaggttta gaatattttt tgtaaaatac
    21961 tctaaaccct aattaatata ctattatttt taatcttaat ttataatttt agtttatttt
    22021 acttttaaca cagtaaattt aagcgtttaa acttcaatac actaatatac taccccatca
    22081 atatctcaac tgattctcta agtgtgctaa tctctcccac atttccaggg aaaattatat
    22141 atggcatatt aggaaatttg ctttctttgc ctgtcatcca tactggtata ccaggtttta
    22201 tctgtcccat tactgtagct ttttttactc ttagtgcttt agtaccaaca tcactagaag
    22261 taattccacc cttagctata ataaaatttg gtcttacatt aagcatacca attatactgg
    22321 taactgcatc tgatatttct acagaaatca taagctgttt atctttatca ttcgtatcca
    22381 agtcaaaacg ctctcgtcta gtataaacag caacagtttt accactgctt attttttcct
    22441 ctacaacttt tataactctt tttacctcat tttttagacc attttcctga agaactaaat
    22501 gttgattaaa ttctataaat tctattggat atttacaatt tttaagttct tctaactgtt
    22561 gggtagtctt attcacatgt gaaccaacaa gtataattcc tccattggta ttaccttttg
    22621 atactaactc atccttagta agcatttcct tatcactcac tccgccaagt acttttgtta
    22681 tggcagcagc gcttctaaac ataaattcct taccactatt tactgctcgt ataaacgcaa
    22741 ttgtaaatac ctttacatct acataatcgg ttgcatttac aataatttta ttgaatcctt
    22801 cagctttttt aagtttttct gttatagctt caatatttag ctctctaagt tctttagaag
    22861 aaatatatat catatcacta gatttgtact ttccatgtgt cttttcttca caccactcac
    22921 ctatatcaga tgatttatat ccaaaagatt tatcctttgc aaattcagtc attcctgctg
    22981 gtacaagtat ttctccttct ttaacataat gtacattatc tatagtaaac ctaccacctt
    23041 ctttaaaaaa tggcattatt atttcaccct gaaataactt ccccgataat ctctctactt
    23101 cgctttttaa taaattagtt tcaacaggat aatgtcctct catagtagaa tcactacgac
    23161 ttatgattat aaaatcctta ttaactttct ttgatacatc cacaatattc tttgaaattt
    23221 ctttatgtgc ttttgtagtt tgagcaacag taaatcccct tgaattagta agtataaaaa
    23281 acatactatt tttctcatta aaaccttgtt ccatactatc cttatcccaa tcagtataaa
    23341 cagaaatatc atgaactgtt tgtacacctg ttgggtcatc atctaataca acaatttttc
    23401 tatctaaatg cttaagctca tctgcaagaa gttcatttaa tctgtcctca tctacttgtt
    23461 taaaagtatt aagtatatct atgctaattg gttttacttc tctcatttaa aacgcccctt
    23521 tatgtaattt gtatagtcta aatgtaattt atataatata aacaatagtt tgttatttat
    23581 agacctggaa gtggtataat tccatttata acactaagca ataaaaccat tataaatgct
    23641 acaactccag caatggttcc tccaattgtc caagagttta aagtatcttt aactgataat
    23701 ccactaaatc ttgaaagtac ccagaatcca gaatcatttg gaagggagaa accaactcct
    23761 ccagcacata tagcaagccc tactaaaact ggtgacattc cagttgccaa aattgtaggt
    23821 cctaaaattg atgatgttgt tactaaggct acagtagcag aaccttgaga aattctaagc
    23881 aaagcactta ataagaatcc aagcactaca actgatatac taagaccaga catagtatca
    23941 actaagaagt tacctatacc acttgcattt ataacactgc caaacgcacc acctgaacct
    24001 gttatcaata atattagccc tgctgaatca gcagcttcaa taactacatc tcccataggt
    24061 ttatttatgt atctctttaa aaatcctatt gccaccaata ttccaagaag tattgctatg
    24121 tttttatctc ctataaatgc aagtactcca ctcattgtag aacctttttc taagaacata
    24181 ctaaatacat ttgatataag tataagaagt attgggaaca ataaagaaaa catagaaatt
    24241 ccaaaacctg gtactttaga agaatcatca tcttcaattt ttatgctatc ttttcccttt
    24301 tctaaatttt tttctctatc attctcatca atagcataag ctggatattt ttcaaaacgt
    24361 tttccataaa tccatccacc tataagaact gctggaaaag caacaattat agaataaagt
    24421 ataaaactac ctacatttgc atttacattt gctgcaactg ctaaaggtcc tggtgtaggt
    24481 attacaagtg catgacctac tatagtccca actcctaagg cacatacaaa aaccattaga
    24541 gatttcttag ttactctcga aacatactta attataggca tcattataac aaaagctgca
    24601 tccataaaaa ctggaattga aattaaaaaa cctgctgtag ttactgctaa agcactgttt
    24661 ttttctccag tcttagctaa caaccccttt gcaatagatt ctgttgctct tgattctgaa
    24721 agaatatttc caaatattat tccaagtcca attacaatac ctatactacc taatgtattt
    24781 ccaaatccag tcgatattgt ggttgatatt tcatttatag gcattctaac cataaagcct
    24841 gtaattatag atgttatcag taatgccaaa aacggattta ctttaaattt aataatcgat
    24901 acaagtaaaa ttcctataga tataacaaat gctatcaaaa gtccatatcc tgtaatcata
    24961 atctttaccc ctttatcatt ctactttatt atttatcaat tttatttttc ttttactatg
    25021 ataacttaac ctcgctgtat ttatataatc taatcctaat aatatgtatt taattatatt
    25081 gatatattta aatattaatt attgtgtttt tttatttcaa cacctgccaa cttttcaaaa
    25141 tactgtgcta ttccactatg gtcatcttcc aaatgtccat gtacttttaa tgtctgcata
    25201 atttctaata attgacttgt cataggtagc ggtacatcta aattgtgtgc tgtttgcatt
    25261 acattcttaa tatctttcaa attaatagat atttttccac ctggtttaaa atcacgattc
    25321 ataatcattg gtatcttcgc atccaatatt gtactaccag ctaggcctcc tcttattgcc
    25381 ttatatactt tttctgggtc tgcacctgct tttgctgcaa gtacaaatgc ctcagaaact
    25441 gctgctatcg tcaaattaac tattacttgg tttgtaagtt ttgtaacact tccactacca
    25501 ttatctccaa ttaaaataga tgagcttccc attatattaa aaaatggctc tactttctta
    25561 taaatttctt ccttaccacc agccataaaa gcaagtgtac catcaatcgc ttttggctcc
    25621 cctccactta ctggtgagtc tataaattct aagcccatat cttttaattt atttgcacac
    25681 aatattgatt ctgtaggagt aactgaactc atatcgacta ctatacttcc ttcttttaaa
    25741 gtttttgcca atccatccat cccaaataat atatcctgaa caatagttcc atttggaagg
    25801 attgtaaata caatatcaca ttcttgaccc atttctaaaa cacttgcgta ttttgctcca
    25861 catgatataa gttcatccac agcagattta ttaatgtcat acaccaaaag attacatcca
    25921 tcttttaata aattttttgc cattggcttt cccattattc ctaatccaat aaatcccaat
    25981 ttcattttat aaccctccat atcttcacat ttttgaaaac gttttttatg ttttagagta
    26041 tactacatac cagtataccg gtcaatataa aatataaaag tatttatttt aacaataaat
    26101 agtaattttt aaagtcattt attatattta gtcatcctat ataagtttta atacatacta
    26161 ttgtttttaa atataatatg ctacttttac agttttgtat ttttactaat ttcaattagt
    26221 tttaattaat ctgcctttaa tgttaatcat acttcacact tcaataattt tttatatttt
    26281 ttaatcttat ctgcaattta aattgcaaaa gattgacctt attttcacta tgttgtatgc
    26341 ttaaaattat ataagttttt gtatttttat attgtttagg ggggtattat taagtatggc
    26401 tacatttaaa agattacaag aacaagcata tgactactta aaagaattga tactatctgg
    26461 agaaatgata gaaaatgaaa tatattctga aacaaaactt gctagtgaaa taggtatctc
    26521 tagaacacct ataagagatg ctcttcaaag acttagtcaa gatggattta tagatataat
    26581 cccaagcaaa ggatttagaa tccatcaaat tacagcaaat gaaattgtag agatattcca
    26641 aatacgctct gcaatagaag ggttttgtac ttttttaatt acaagtcaat acaaagaagc
    26701 tagggcagta gaaactataa gtaaattaaa acatctttta gataaacaaa aagatatttt
    26761 actaggtgat aaaaatttaa actcatttgc agaatacgat acattatttc acacaactat
    26821 agtttcatat gctcaaaata ctgaatttga caaaatgttt aacaactata tgtacagaat
    26881 aaaaaaatta gctctcgatt cattatcaca tgaaggaagg cttgaaacaa cattaaaaga
    26941 acacacagat atttttaata atattgcaaa tggatgtatt gaagatgttt acaaaactac
    27001 tctcattcat atggaaactc ctaaatacat taatttagaa gatttttgta acaataagtg
    27061 attttagtat tatactttca tttattaaaa atatatctac ctcaaattaa tttcctaatt
    27121 tataaaaaat attctataat tatattgtaa ctaatactat tctaaggagt gatttttttg
    27181 aacgaaaatg ttgtaactaa tgaaactatt gacaaagatt atgctattga agaagttcgt
    27241 atggcatgca aacattttgg tgacttatat ttttattttt ccaaagtgtt atttgaagag
    27301 tttggtgaag ataaaactat cgaaatacta agaaaagttt tatttgaaag gtctgaagaa
    27361 agagctattg ctatgagaga aagagctctt gaaaatggag atgaattaat tgcagacaat
    27421 attattagca caactgatgt tccatttcta ggatgggttc ctgaatttaa agagttacat
    27481 tgcccttatg gagcttcctg gcttttaaga tttgaggaaa acccttggtt taaaaaattc
    27541 gcttctcttt actgtgatgt tacagatact actgtagctg aagtatttac aggagatacg
    27601 tcccataaaa taacaaaaaa cattctatgg ggagataaaa gttgtgaaag aatatatttc
    27661 catgatgaca aggtatctga aggtaaatat acttatggaa ataaaaactg taaataaata
    27721 atttaaattt tctatattca tatactatat tttgtacttt ttcatataaa tattataact
    27781 agtcttttat tttacttttg tttaacctaa agataattta cttttaatct atatgaatta
    27841 tcatataaat aatcagaaag ttatctttat aaattatagt tttagattta taatttataa
    27901 aatatttatg ttctttgtaa aattttttag aagcttctaa tgttatgcta taaaactatc
    27961 ttgagaaggg ctgattgaat gtttaataaa atatatgaaa agaaagaatt tatagttttt
    28021 caagttaaaa aaggttatgt agtttataat actagaaaga gttttgaaga aggtcataca
    28081 catttaaagc attttgaagc agcaaagaca gctattgatt tagcaataaa taaaaagatt
    28141 cctagaagca aagatggata ttatctgact agtttaattc gcataagtga tgatggttat
    28201 tacattgata agttaagcga attgttatat gttagagaac aaaaaggtaa aaaagaaaag
    28261 tactgtaatt ctggttatca gatgtaacaa gactagttgt gaattataag taccaatcat
    28321 aaataccttt agtcttaaaa tcaaagagta tcagtaaatc tataaaaaaa caacttactg
    28381 atactctttt taattatatg attaatattt gaatttgata caaaatttta tacaaaagtt
    28441 tatgttatct attctgattc tttgtctgca aattggctat tataaagctt ctcataaaat
    28501 ccaccctttt ccattaattc atcatgattc ccctgctcta taatacttcc attattcata
    28561 actaaaatca agtcagcatt gcgaattgta gaaagtctat gtgctataac aaaactagtt
    28621 ctaccattca taagatttct cattgctttt tgaagcataa gttccagcct agtatcaaca
    28681 gaactagtag cttcatcaag tatcaatatt gaagggtctg atataaaagc tctagctatg
    28741 gttaaaagct gtttttcccc tagagatata ttagatgctt cttcatttaa gaacatattg
    28801 tatccatctg gaagagtggt tataaagtga tgaacatttg ctacttttgc tgcttgtata
    28861 atttcttctt tagtagctcc aaatctacca tattctatat tttcaaaaat ggttccatta
    28921 aataaccatg tatcttgaag caccatacca aacattgaac gcaaatcttt acgtttcata
    28981 tctcttatat ctactccatc aattttaata gcacctttct ttacatcata aaacctcatc
    29041 aataagttta ttaatgttgt tttacctgct ccagtaggtc caacaatagc taccatctga
    29101 ccacttttta cttctgcatt taaatcttct attaaagttg aattttctcc atatccaaaa
    29161 ttaacatgct caaatgtaac atttcctttt acattttcta tcttaactgg attttcaaca
    29221 tcaggtattt cctcttcttc gtctaaaatt tcaaatacac gttctatagc tgcaaaagaa
    29281 gattgtatta ctgtagataa ctgagtcatt tgtgaaagtg gctgatttat ctgccatata
    29341 taacgcacaa atgcttgtaa atttcctact gttatccctc cacttactgc atatatagaa
    29401 ccgacaactg ccacagctgc aattccaaag taagttacta atgaaaccat aggattcatc
    29461 atacttgata taaattgtgc cttaaatcca ttttcacaaa gttcttgatt tactttttta
    29521 aattctttta tagaatcttc ttctttacca tatagtttta tttcattaaa tccagtatac
    29581 atttcttgta cttttccatt taattttcca agagcatttt gttgcaatga aaatctactt
    29641 tgagattttt ttactacaaa tttagataca aaataactaa ttggaattat aaaaagagct
    29701 ataagagcca tatatatatt aattgtaaac atcattgaaa gtgccaaaat tatgactaag
    29761 attgagttta caatttgcat aaaactctgt tgcaatgcat ttgatattgt atctacatca
    29821 tttgatatac gacttaatac atctccaaaa ctattactat caaaataact aataggcaat
    29881 cttcttattt tattttcgac ttcatttctt aaatctctca ttgtgttttg aatagcattc
    29941 gtaagtagaa aacttgaagt atacgtaaaa attgcattag caaaatatat acatgctaaa
    30001 atcaccaata tttttgttac ataatcaaaa tttacggaag cccctggtac tccttttgcg
    30061 atactaatta catctttagt caattgtgtt attatcaatc cttcagtttt aggtgcaagt
    30121 gcattcatag tagctgctga aattatacat ataatagcta taataaagga aaatttatat
    30181 ttctttacaa atggaagaag tctaccaatt gttttcttaa aacctttaga ctttttcatt
    30241 attctaattc ctcctttttt aactgagatg ttgcaatttc atagtatatt tcacagtttt
    30301 ttaataattc tttatgagtt cccattccga caacttgtcc ttcattgagt actataatct
    30361 tatcagcatc aataatagaa ctaattctct gggcaacaat caatacaatt gcatcttttg
    30421 tttccttctt caatttagca cgaagtattg catccgtttt aaagtcaagt gcagaaaaac
    30481 tatcatcaaa aatgtatatt tcaggtcttc ttataatagc ccttgcaata gaaagtcttt
    30541 gtttttgacc tcctgataca tttgctccac cttcactaat caattcatca aactttctag
    30601 gtttttttga tataaactca tatgcttgtg caactcttgc ggaatattct aattctgact
    30661 ctcctgcctt atgtttccca aatctaatat tatttgctat gctaccagta aaaagcagtg
    30721 atttttgagg tatgaatcct atcttttttc ttaaagcttt taaatcatat tctcttatat
    30781 ctactccatt tatcttaatg ctaccctctg ttacatcgta aaatctaggt atcaagttaa
    30841 ttagagtact tttcccacta ccagtacttc ctataaaagc gactgtttca cctgtttttg
    30901 cagtaaatga aatatcctta agtacagaag cttctccatc tggataggta aaagttacat
    30961 tatcaaattc tatgataccc ttattttcag tatccttaat tccattttca ggatttttta
    31021 tcaatggttc ttcatttaaa agttctttta ttctatttgc agatacctct gctcttggat
    31081 acataataaa taccattgaa aaaagcataa ttgaaaacat ggcatggaaa agatattcca
    31141 aaaatgcaac caactgtcca acttgaagct ctccaatatt tatcttctca cttgaaatcc
    31201 aaaatacagc caaaacagct atatttaata gtaaaaagaa tgctggttga gtaattgaca
    31261 taagcttaaa tagtttctta gaaacattag cataatatgt atttgttttt tcaaatcgct
    31321 ctgtttcata atcatcattt ccaaaagctc taattactct tattccagtt aaattttctc
    31381 tcgatataag gtttaattta tctagtcctt tttgttgttt tgaagatatt gcctgagata
    31441 cttttgctat tattgctact ccaataatta taaaaggtac tgatatagcc aatactatag
    31501 aaagttccac acttgttcta attgtcatta ttatacttat gatgaacata actggagtta
    31561 ataaggcagt ccttagtagt atattaacaa attgttgaat ttgaaatgca tcattcgttg
    31621 ttcttgtaat cattgaagaa attccaaatt tattgtattc agtatgtgaa aattcttgtg
    31681 atttctcaaa tatgtcattt cttatatctc tggttacact tgttgatacc ttagcagaac
    31741 aataacccaa caatatacta cctacaaccc ctataataga aataataaca atcactattc
    31801 ccatttgttt tatgtaagct atattctgat ttgcaatacc attgtcaatt attttagcta
    31861 ttatagttgg aatccccaac tcgactaatg caaaactaaa tatagccagt atgtttaata
    31921 ctattaataa cttataattc tttaaataac ttaaaattaa tttcatattt ttctcctttc
    31981 ttaatttttg agatagtgta atcatgtaat aatactaaat ttataaatgc ttaattttat
    32041 aaaaaaagga gaaaagagtt aacgcactct cttctccagc atattctttt taatacaccc
    32101 tccgacaagt cgcttatatg ttaacatatt tgacaagtaa tgtcaattca tatagctaaa
    32161 agtcttttaa atgcttcatc tcataacatt gtaacaaatt ggcatgtaat ttatttttgt
    32221 tttttactgt attccaattt tacaagttct ccaatattac taactttctt aaaattcaaa
    32281 ttagtcttat ctataccaga cttaaatagt gaaatccctc tccccaatat tattggcaat
    32341 atatatatat aataatcatc aattaagtta tctttcataa ataattttac tatatcgcct
    32401 ccaccaacta tccaaatatc ttttttatcc ttcttttttc caatttgctc aattaatttt
    32461 ttaggttcta aattagtata ctttacattc tccagttgtc ccattaattg tatttgagta
    32521 tatatagcat tccttaccct tatagtacca ttcatttggg cttaattcat ttgcaatctg
    32581 tacatatgtg gaatttccca taatgatagt atcaattgta tcgtaaaaag tattataatc
    32641 atcttcctgc tcattcttaa attctgaaga tatctcgtct aaaaaactta cactgccatt
    32701 ctcttcagct atataaccat ctaaacttgt ggcaatataa agtactactt ttctcatgtt
    32761 tactacctcc aacatttata gtgtgattat tctatgtaaa catcactttt ttactcattc
    32821 aataataccc tattaataaa tcctcaaact aatttaaata aactttgcat aaaactaaaa
    32881 atatattata taaataattt aaaatcacac taacttattg aaaattctaa ttattacaaa
    32941 taaatattgc tctctgttaa cgctagtttt ataatataat tataagctat taatattata
    33001 aagccgatat tactaatata ataattatag ttaatttact tttgttgaaa ggtggtaaat
    33061 aacatgaaaa tttcagaagt cgctactcca aattttttgc tagatttaga ccagttagaa
    33121 aagaatatag ataaaattca acaaatttgt acaaataata ataaacaact ttggcctatg
    33181 cttaagacac ataaaagtac ttacatagcg agacttcaaa aaaatgcagg tgctagtggt
    33241 tttctagttg gaacacttga tgaaggtgaa gctctgataa aagcaggttt tgaagatatt
    33301 atgttggctt atccttacat gggagaagta aatataaata gaattgtgac tatggcatca
    33361 aaaataaatt taatctgtgc tacagataat atagaatgtg ctaaggctta tagtgaagct
    33421 ttttctaaat caaaaataga gtgcaagcta cttattattg tagattgtgg cttacaccgt
    33481 tttggggttg aaccagaaaa agtggttgaa cttgctactg aaatttctaa gctagaaaac
    33541 atcaagattg ttggtataag ttcgcatcca ggtcaagtgt acggttgtag ttctccagaa
    33601 ggtgttccag atgtttgtgt tcaagaagat tcttctatgg agaaagcata caaagctcta
    33661 actgataatg gatttaatat agaaattgtc gcttctggta gtactccaac agttgaagct
    33721 gaagctgctt ctaaaactat aactgctgta agacctggaa actatgtata ctatgatgct
    33781 atacagatag gtttaggttg tgctactgaa gatatgtgtg ctcttactgt tgtgtgtact
    33841 gtaatctcaa aaaatagcag tggatattat ttaattgatt gtggaagtaa gtgccttgga
    33901 cttgataaag gtgcacatgg aaatagtagt attgttggtt atggtagggt atgtggacat
    33961 ccagaactta ctattgattc actttcagaa gaagttggaa aaattaaagt aaatggtgaa
    34021 actgatataa aaattggtga tagaataaga attataccta atcattcttg ttccactgca
    34081 aacttgacga gtaatttatt aggttttaga ggagatacaa tagaaaaagt catagaagta
    34141 gatattcgtg aaaattcaaa acaaatcatc ctttaaaaat atatttattt tgatattaac
    34201 atcatgaaag tatatttttc ttaaaattat tgttaataac taatgttaat gttggataaa
    34261 taaaaaacat aaattctaga aatataaatt taaaaaaaga ggctgtctca aaattggata
    34321 tgtccctgtc aagtagacag acttaaaaaa ggctatcttt atgcggaatg agttcgatat
    34381 tcaatcggac tcattccatt ttttaattgt attctttcat tattataaaa ataaatataa
    34441 tcatttatta gactaatcat ttcctttgga tctgtagtat caattaaata tattaattca
    34501 gatttaaaat gactgaaaaa actttctata ggagcattat cccagcagtt gccccttcgg
    34561 gacatagatt gtataatacc attatcttta agccttttag agtaagatct acttgtatat
    34621 tgtgaacctt gatctgtatg taaaatacaa tctttagcta aattcatatt tattagttga
    34681 tttactgttt tattaactaa tttagtatcc atttttgtac ttatctcata tgccactatt
    34741 tcaccattaa ataaatcttt ggctacatt
//
LOCUS       DAMKPG010000034        33718 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMKPG010000034 DAMKPG010000000
VERSION     DAMKPG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.037"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(162..1430)
                     /locus_tag="PC621_001754"
     CDS             complement(162..1430)
                     /locus_tag="PC621_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108913.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            complement(1441..2253)
                     /locus_tag="PC621_001755"
     CDS             complement(1441..2253)
                     /locus_tag="PC621_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108914.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            complement(2262..3461)
                     /locus_tag="PC621_001756"
     CDS             complement(2262..3461)
                     /locus_tag="PC621_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108915.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            complement(3473..4297)
                     /locus_tag="PC621_001757"
     CDS             complement(3473..4297)
                     /locus_tag="PC621_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108916.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            complement(4290..5006)
                     /locus_tag="PC621_001758"
     CDS             complement(4290..5006)
                     /locus_tag="PC621_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108917.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            complement(5078..5434)
                     /locus_tag="PC621_001759"
     CDS             complement(5078..5434)
                     /locus_tag="PC621_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4108918.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            5790..6248
                     /locus_tag="PC621_001760"
     CDS             5790..6248
                     /locus_tag="PC621_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF4108919.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            6252..7214
                     /locus_tag="PC621_001761"
     CDS             6252..7214
                     /locus_tag="PC621_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108920.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            7208..7957
                     /locus_tag="PC621_001762"
     CDS             7208..7957
                     /locus_tag="PC621_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108921.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(8023..8325)
                     /locus_tag="PC621_001763"
     CDS             complement(8023..8325)
                     /locus_tag="PC621_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4108922.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(8464..9096)
                     /gene="thiE"
                     /locus_tag="PC621_001764"
     CDS             complement(8464..9096)
                     /gene="thiE"
                     /locus_tag="PC621_001764"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF4108923.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            complement(9119..9907)
                     /gene="thiM"
                     /locus_tag="PC621_001765"
     CDS             complement(9119..9907)
                     /gene="thiM"
                     /locus_tag="PC621_001765"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF4108924.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            complement(9931..10725)
                     /gene="thiD"
                     /locus_tag="PC621_001766"
     CDS             complement(9931..10725)
                     /gene="thiD"
                     /locus_tag="PC621_001766"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF4108925.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     regulatory      complement(10825..10917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11010..11321)
                     /locus_tag="PC621_001767"
     CDS             complement(11010..11321)
                     /locus_tag="PC621_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF4108926.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     gene            11612..12856
                     /locus_tag="PC621_001768"
     CDS             11612..12856
                     /locus_tag="PC621_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108927.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            13053..13922
                     /locus_tag="PC621_001769"
     CDS             13053..13922
                     /locus_tag="PC621_001769"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF4108928.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(13976..14164)
                     /locus_tag="PC621_001770"
     CDS             complement(13976..14164)
                     /locus_tag="PC621_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF4108929.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(14324..14914)
                     /gene="cysE"
                     /locus_tag="PC621_001771"
     CDS             complement(14324..14914)
                     /gene="cysE"
                     /locus_tag="PC621_001771"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF4108930.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            complement(14941..15849)
                     /gene="cysK"
                     /locus_tag="PC621_001772"
     CDS             complement(14941..15849)
                     /gene="cysK"
                     /locus_tag="PC621_001772"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF4108931.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            complement(16086..16712)
                     /locus_tag="PC621_001773"
     CDS             complement(16086..16712)
                     /locus_tag="PC621_001773"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF4108932.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            complement(16731..18788)
                     /gene="kdpB"
                     /locus_tag="PC621_001774"
     CDS             complement(16731..18788)
                     /gene="kdpB"
                     /locus_tag="PC621_001774"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF4108933.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            complement(18813..20495)
                     /gene="kdpA"
                     /locus_tag="PC621_001775"
     CDS             complement(18813..20495)
                     /gene="kdpA"
                     /locus_tag="PC621_001775"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF4108934.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            complement(20536..20628)
                     /gene="kdpF"
                     /locus_tag="PC621_001776"
     CDS             complement(20536..20628)
                     /gene="kdpF"
                     /locus_tag="PC621_001776"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF4108935.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            complement(21039..21671)
                     /locus_tag="PC621_001777"
     CDS             complement(21039..21671)
                     /locus_tag="PC621_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF4108936.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            complement(22043..23005)
                     /locus_tag="PC621_001778"
     CDS             complement(22043..23005)
                     /locus_tag="PC621_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF4108937.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            complement(23026..23976)
                     /locus_tag="PC621_001779"
     CDS             complement(23026..23976)
                     /locus_tag="PC621_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108938.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            complement(23999..25495)
                     /locus_tag="PC621_001780"
     CDS             complement(23999..25495)
                     /locus_tag="PC621_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF4108939.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            complement(25508..26692)
                     /locus_tag="PC621_001781"
     CDS             complement(25508..26692)
                     /locus_tag="PC621_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108940.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            complement(26685..27635)
                     /locus_tag="PC621_001782"
     CDS             complement(26685..27635)
                     /locus_tag="PC621_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF4108941.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            complement(27637..28731)
                     /locus_tag="PC621_001783"
     CDS             complement(27637..28731)
                     /locus_tag="PC621_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF4108942.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            29206..29475
                     /locus_tag="PC621_001784"
     CDS             29206..29475
                     /locus_tag="PC621_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108943.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            29593..30345
                     /locus_tag="PC621_001785"
     CDS             29593..30345
                     /locus_tag="PC621_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4108944.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(30548..31813)
                     /gene="hisD"
                     /locus_tag="PC621_001786"
     CDS             complement(30548..31813)
                     /gene="hisD"
                     /locus_tag="PC621_001786"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF4108945.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(32078..32572)
                     /gene="cdeM"
                     /locus_tag="PC621_001787"
     CDS             complement(32078..32572)
                     /gene="cdeM"
                     /locus_tag="PC621_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF4108946.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            complement(32686..>33718)
                     /locus_tag="PC621_001788"
     CDS             complement(32686..>33718)
                     /locus_tag="PC621_001788"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF4108947.1"
                     /translation="NKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLAECGD
                     ELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILSKMYD
                     ENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYLEGIT
                     DIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNAVIVN
                     GDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVREKCGA
                     LLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDKGYCE
                     KMNKMLKIS"
BASE COUNT        11232 a         5822 c         3654 g        13010 t
ORIGIN      
        1 ctttttttca ttatcaaaat aaatctaaac aatgaaaata tatttgattt catataaaaa
       61 aagtgtctca tgaactaaaa agtttatttt gagacactcc cttacttata cttatctgtg
      121 atacatataa aagtttaaag ttctaaaatt aattctaata cttaatttgc aacaaaaaat
      181 aacttttctt gtaaacttat atctccattt attagctcag cttcataaat attcttttta
      241 agtttcatat caaatacttg aaattttact ggcttagttc cgactacatc aacaatccct
      301 ttaaatcctt ttaattctcc tgatatactg ttcttcatac ctgaaataga ataaatctta
      361 tcatccagta acttaacttt cctaactcca gatgatgctt cattataatc tacttttaaa
      421 tcaagtatct cataatcttc taatttaatc tcattagaat taatatcata aacaagattg
      481 attatggaat tactcatatc cgtaactata agattcaatt tattattttt tatcatataa
      541 tctagcttag ttttacttaa taagccaatc tcctttggat ttgcattctt ttctttgttt
      601 agattatcca taaatttatt acttatatta aaagtgttat cttctatatc atatataata
      661 aggttagagt aactatcatg tccttcaata tctccatttt tacttttatt atttactatt
      721 tctaagtata atttacttcc aatagaaaat ttaatttcat tgatattttg ttgatatcca
      781 ttctgtaatc ctaaattaaa tctatttaat aatttataag acttattttt tatatctatc
      841 tttaatatac aaatattatt gaccttagaa cctgaaatat tagtttcact aattaccata
      901 tataaattgc ctttatatat tttattaaac aaggttttcc aactgttttc tttaaaatca
      961 gacttattta aattaatatc aaaattttct tctaccccac tttttaaatt tttatttgaa
     1021 acctttatac aatcatttaa agcattcatt ccttgtacta aatcagactt aacagaggta
     1081 tacgcttctc ctatattcaa atcatcttta tatataagtt cttttccttt atcattaaca
     1141 aaatcactgt attttttgtc aatatttaaa taatcatttc taattcttga tttggggttt
     1201 ttattatttc ttactccatt tttacttata attacctcct cataagaaaa tttgcttata
     1261 ttaggtgtat atactacact tactctccct atatctttta cattgccttt taaagttttt
     1321 acagatagtt tatccttatt aaaataactc aatattaagg tcatagtaat cattaaaaaa
     1381 catactatta taaaacatat tgtatatttt cctatatttt ttgctttcat aatatccctc
     1441 ctaaaagtca atcttttgct tcaaaagttt attagatata agactacata caaatatgtt
     1501 tatacttgaa tatagaacag taaatacatc actgtattca taaggtataa taaattctat
     1561 agaaattata aacactaaaa tttccaaaac taaaaataga aaacttatta aagtgcttac
     1621 tttataaata gatttattta taagaattga tgtaaatact acagatacaa atcctatcaa
     1681 taaaaatata tactctacaa atagtatttc aaagctatat ggaagtaaca tttttaactc
     1741 atatattgtt tcttgtacaa aaccatagtt tgtgacatta ccaagcatat gtctaggcaa
     1801 caacttactt gcaataaaca aagatattgt aagtactact gtatagctat acactagaaa
     1861 taatatattt aataatttag atatataaat attgattcta tttttaggta gcattataag
     1921 tgtatatata gatttactcc taccattaaa gtctctatac caaataaaaa aagcatagca
     1981 aatacatgca attataccaa atataaacca cgattctgta gaacccatta tatttctaaa
     2041 gcttgagaaa cctgtaacat ttaccgtttc tatcgtattt ccgatttttg ttaactgttc
     2101 acttttaact gcattttcta ttatgtaatt atagtcattt aaatttttta ctatgtttaa
     2161 aattacaaaa gaacaacaac aaattattat aaatatataa ttctttatat ttctttttaa
     2221 ttcaaattcg aataatctta ttaaatcttt catcaactca cctactttat ttcaaattct
     2281 aacatactat ttgaactgtt aaattgtaat tctccttcat atacaatttc cttagaattt
     2341 ttatttatta tatatagcat aaatttatta tcccaagata atatagagtt tagccttgat
     2401 gtatatgcaa cgattttatt tgaatcaatt tcaatacttc taattcctct tttttctcta
     2461 ttatctaaaa catatttatc ttcatctaag cttttatcta atatattata ggtaagtaaa
     2521 tttaactctg taattgaact attatcataa tcattcgaca ttaccatata taatttatta
     2581 gcttctaact ttgcttttgc taaagccatt ttgctatcag tttttaattc cattatgctt
     2641 tccttatttt cttcaagatt atactcaagt aaatgaaatt tagaattact attatcacct
     2701 atgtttgtat ttagtacatt tactaaaata tacatcttat cctcatattt gctagcataa
     2761 cttacaccta tgttctctat gtcttggtat ttctcggaaa aataattact taaatttata
     2821 ctttttatag tcttgatttt tttattttct ggattaattt ttattatatc tatagtactt
     2881 gaatattctt tgttattttc tgatgaatca cttgtagcat aaagtacaga tacatataag
     2941 tattttttat atctttttga tgctagtata tttgcataat aatcatctgt acttttatct
     3001 actttatatc ttgtccttgt aaaattattc atatctaatt tttcatattc aatttcttta
     3061 tcactacctt ctgatggaaa tacacctttt tctaaatctt cttttttctc atctatcaca
     3121 aagtttatat cttttgactc ctcattcatt tcaaatccaa catgagcaat taaatctgaa
     3181 ccactagata catcaattaa attatttgtg ttcttactat ccttttgtag ttttattaat
     3241 tcagtctttt tagttttgtt acttccattt attgtataat atgaatttat atatccattc
     3301 aaagcatatc caaaatttat gtttatatgg tctaattcat tagagtcacc ttttatagtt
     3361 tttatcttta aattatcttc attatttaat aaacctatac taaaatatat tgctaaagtc
     3421 aaaaatataa taagtataga tattttagat tttaatttca taccacccct ccttaaagag
     3481 atactttctt tttaattagc aaataagata ttccatttag aataaacatt ataattaaaa
     3541 aacttaataa ccctgctact aaattgttaa attgaaatcc tataagatta taatattttc
     3601 taaataaatt gattatattt atatatattg ttgaaaaata taagcctgct ataatcagca
     3661 aagtgattaa aattacataa aatttattta tataggaaaa gataaataat atacagcaat
     3721 ttacaataga aatagctata agtagtaaaa atacataaat agccataaaa gagataaaat
     3781 caattggtat actcttcaag tttaaataat ataaatcttt ggcaaagttc atattgatta
     3841 taggaatatt tctaaaaatg aaattaaata tatatttaca tataaataat gttgctgtct
     3901 gacttatcaa aaaaccatat atcattgtta tcaaaactat tgatttagat atacaaatct
     3961 taaacttatt ttgaggtaac atcataagtg tataaatact ttgattattt gaaaagaact
     4021 ctcttttcca cataaaaata tagtatataa aaagtcccaa cattccaaag aacaatatat
     4081 tttggataaa accaagatta ctaagtatcc ctagtccata aaatatacct ccaaaatctt
     4141 ctattgctaa gttcataata aatttagaat ttttaaattt attaatttcg aataaattta
     4201 aactaagtgt aattaatgaa aaaaatgata tataagctat ataaaactta ttgcttcttc
     4261 tcaaatctac atttattaga tatagaagtt tattcattct tatatacctc cctcatctta
     4321 tctaatatag atttaccttc tctttctctt aattcttctg cattaaatga agttataatc
     4381 tgcccttcat caagaattac aacctcatca actaaacttt ctatctctac tatctcatga
     4441 gtagttatga taatggcttg attttcctcc atatattgcg ctattactcc tataaactct
     4501 tccctcttaa acaaatcaat tcctgtaaat ggttcatcaa gaagtatgta ctctgggtct
     4561 tggcaaaatc caagtattaa ttttactctt gcaatgtttc cttttgacaa gttatcaatt
     4621 atttcatctt catccaattt aaatatgtcc atcatttctt ttgatttttc tttattccac
     4681 tttgaataaa aaatttccat aaattcaaat gtttctttta ttgttgtatt gctaaagtga
     4741 gattgtacat ctggaacaaa agccaattta ttgtatacct tttcatttat tttttctcca
     4801 tctatacgaa tttctccact atcaatgttt ataagtcctg ctattgcttt taacatagtt
     4861 gatttaccaa caccatttat accaagtaat gcagttattt ttcccttttc tatattaaaa
     4921 cttacatttt ttaaagcttc tatctttctt attttaagcc cttgcatcct tttatatgtt
     4981 ttgctgacat tctttatttc tatcatattt actatcctcc acatccaata tgcttataaa
     5041 taaactttct aaactaagtt ttctatttgt taaaaaacta atacttatct tctattattt
     5101 ttaaaacttc ttctttgcta acatttattg ctttcatatc ttctataaaa acagatagtg
     5161 attcatttat aagctctaat tttaaatttt ttaatatatt ttcatcaact gttacactgc
     5221 tttgataatt tttaaaagta tttattatat tcatatcacc catctcctta tatgctcttt
     5281 gaactgtatt taagtttact tttagattaa gtgccatttc tcttcttgat ggtattgtct
     5341 ctcctggttt caactctcct gttactattt gtctctttat atactttact atttgcaaat
     5401 aaatgggagt tgtattatct aattcaaaat tcatgattcc tcctattcta gtgtattaat
     5461 acgatagtac acttacttta aaaaaatacg cctttatttc agactttatc tagtatccta
     5521 ttaagtgtat tatacgatta atacacttaa tttgtaaata cttattttat aaattgcttt
     5581 atttcaattt gctaaaaaat ctaaagtaaa gcttaaataa tagctttaca aaatggagca
     5641 tcaaatgaga ggatatatat tttatgttat gttagacttg aaaacaagca aacgagataa
     5701 gattatatgt tttcaactaa tcaaaatctt atatgtatta atgcttaaaa atctagtaag
     5761 aatatgaaca cacattagga ggtaaacaaa tgaattgcaa aattgaaatc aaaaatatcg
     5821 aatcagtcac agtggctacc atgcgttata atggacctat gactgaggca acaaagtatt
     5881 ttccaaatgt atttaaagct ataaaaggaa agtctaacgg tgcaccattc ttttgctact
     5941 atgatgttga ccaaaaaact ggtgttgcag aaatggaatt atgtgttcca actgctgaaa
     6001 ctccaaatcg tatgggtata actacaaaag aatttcctaa aacaaaagct ttatttttta
     6061 ctcacacagg ctcttatagt aatttgccaa aaacgtatga aatgatattt aaatatattc
     6121 atgaaaataa tataaaaata caaactcctt ggagagaagt atttattaaa ggaccaggta
     6181 tgttgataaa aggaaaccca gataactata ttacagagat aatatttcca cttaaagagg
     6241 aggaataata tatgttagcg attgaaatag acaatttagt aaaagaatat aaaaatggtg
     6301 tgaaagcttt aaatggtcta agctttaatg taaatgctgg agaaattttt tctttacttg
     6361 gtcctaatgg tgctggtaaa tcttctctta taaatatttt gactaccttt tacaaaccta
     6421 catctggaaa tgtaaaaatg tttggaaaag atttagtaga taatccttct tggatacgta
     6481 ctcaaattgc ttgtgtagca cagcaaatct ctattgatga acatttatcc cttatggaaa
     6541 atatggtatt tcaaagtaaa atgtataaag tagaaccaca gattgcaaaa caaagaatag
     6601 actctttaat tgataagttt gacttatcta gttacttaaa atatcctact tcatcatatt
     6661 cagggggtgt aaaacgtaga ttggacatag ctatgaatat ggtatcatct cctaaaatcc
     6721 tatttttaga tgaaccaact gttggtatgg atgtcgattc aagaaaatct atgtgggata
     6781 tgttattaaa aattagagat gagtacggca caactatatt tctaactact cattatcttg
     6841 aagaagctga acaattgagt gataatattt gtattatgaa gaatggaaag gatttagcac
     6901 aaggaacacc atcaagttta cgtagttaca ttagacaaaa tatactacgt ataactttcc
     6961 ataatactga agatataaaa aaatataaag attcaattaa aagtactggt ttagtaaagt
     7021 ttatgagcgt acgagaaaat tcaattttta taagtgtaaa cgatagtaga actgctttta
     7081 ctttaataaa taaatggctt ttagaacatg atatagaatt tgatgcaata gaaattgtag
     7141 agccaagtct cgaagatgta tttttagcac ttacaagttc taaaaaaagc ttaaaggagg
     7201 aatgggaatg ttaaatatct tatggcgtag tatgaagtgg cgttttaaaa atccaatctc
     7261 atttgtagtt actatattac aaccttttct ttggcttgtg ctttatagtt ctattgcaaa
     7321 ccaaactatg aataatataa atatcaataa ttatactgct tttatactac caggtatcat
     7381 tgtactggtt gtgttttctt cttgtagtag tggtggtatt ataaacttta taatgaagaa
     7441 cagtggaagt ttctatagag tcttaattgc acctatcagt aggtattcaa tcgtccttgg
     7501 tcaattatta gaagctatct tagtttcctt catagaagta actattttgt gtatagtaag
     7561 tatattcttc tcagtaagga tagaatctgg cattggtgga atcctactta tgatagtact
     7621 gatatttatg acagccttct ttttatcaag tctagcttat tctataagtt tattattgcc
     7681 aaatgaaata gtttatgaaa caattatgac tgcaattgta cttcctattt tctttttaag
     7741 ttcagctctt ttccctatag aaagtttatc tggaggatta aaagtagcag tgatgctcaa
     7801 tccatttacc catgttatta acgctttacg tagcttaata tttggtgaga ctattcttat
     7861 tggagatata ctgcctgtta tcctactatt tttaatattg tgctgttcaa gtttttcact
     7921 tgctatgtgg agattaaaaa aagaaatggt aagctaagtt ttaagcctat taataacaaa
     7981 taataaaaat ggactctatt atatagaatc cacttttatt atttatttta atatttctaa
     8041 aaccgaattt aacacagcat gaccattaca agtaccatag tttaccatat ctattataga
     8101 tactggaact ctatctccaa ctatagactt tacgtcatca agcataaatc taagttgtgg
     8161 tccaagaagt aaaatatcaa agttatcaag ctccgatttt atatctcctt ctccaactgc
     8221 ccatatttta cagtctatat ttcttttttt tgcatccttc tccattttat ttaccaatag
     8281 ggtagaagac atgcctccaa cacacactaa caatatctta atcatttata gctccttttt
     8341 aaccaaatct acatatatct tgattaaaaa gtatatgtag aaaagtttat catttataat
     8401 tgcttaaatt ttactcttag attatatcaa atttttccat ttttttcact accagagcca
     8461 attttatatt tttaatgtcc acttttccac ttctttagag atatcttcag cttttaatat
     8521 agctgatatt atagcatacc cgtcactttc taaacattta agtttatcta tattgtctaa
     8581 atttatacct ccaattagca caaatggcat gtctactttt tctttaattt ctttaacagt
     8641 ttcaaacgaa gctgattttg catctaattt agtagatgtt gtgaatatag accctacacc
     8701 taaatagtct gcgccatcat tttttgcaca agtagcttct tctaaagttc ttgctgatac
     8761 tcctagaatt ttattgtttc ctataagctt tctaacctct ctcacatcaa tgtcactctg
     8821 tcctacatgt actccatctg catcacaaat aagtgctata tccactctat catttattat
     8881 aaacttaaca ccatatcttt cagttatttc tctaagtttc atagcttttc ttaaaaactc
     8941 tcttccagaa gcattttttt ctctaagttg tacagttgtt attccagaag atattgcatc
     9001 ttcaagacat ttataaaagt ctctaccttt aagcatttca gagtcagtta ccaaatacaa
     9061 ctttaagcat ttttttaaag attctttatc aatcatttta agtctccctt actctttatt
     9121 aatttaaaaa ttcaatatta gaatttttac ttaaagtatc tatatccatt tgataaatag
     9181 cattcataag attttcttta aatgtaccta ttggtggatt atttaaatta gctaattccc
     9241 cacttaaaga cattgtaaga acacccattg ttgctgcttc taatatattc tcagtaacag
     9301 ccataaaact tgcaatcaaa ctagcagtca tacatccagt tcctgtaatt ccttttaatt
     9361 tatcagttcc attgtttatt ttatatatac ctttaccatt agttattata tctatttttc
     9421 cagttgctac aactacacat tcaagctttt cagcaatctt tcttattatc tcagtagaat
     9481 cttcttcttc atcaaatgaa tctacacctt taccccttac attaaagcca cctataaact
     9541 ttatttctga tacattccct ttaacaacac taaatttgac ttcatttaaa agtctacttg
     9601 taagttctgc tctcgctttg cttgcaaata ctccaactgg gtccaaaaca acaggtttat
     9661 tgtacttatt tgctgctttt ccagctaata gaaataaatc caacatattt gagttcatag
     9721 ttcctatatt tattactaca gaatttgcta cacttaccat ttcttctact tcctcatatg
     9781 aaaaactcat aagaggactt gctcctacag ctaaagtcac attagcacaa tcatttatag
     9841 ttacattatt agtataatga attaccaatg gatttaattt ttttacatct ttaattaaat
     9901 tatacatatt ctacctccaa tattttttta ttactcaaat ttataaaaat gatgtactgg
     9961 tcctactcca tgacctatat caaaacttct ttttattgct tctgttatat agattttact
    10021 tagatttact gcttctgtta tttcatatcc taatgctaga taagatgtta ttgcagaaga
    10081 taatgtgcac cctgttccat gtgtattttt tttatcaatt ctttcacttt tataaacttt
    10141 aaatatattt ttaccaacta aaatatccac tgcttctcca tctaaatgac ctcctttcat
    10201 aagtacaaat ttaggtccaa gttgtagtat ttcttctcct actctcttca tatcatctac
    10261 attatgtatt tttattcctg ttatttcttc tgcctctggt atatttggtg taattatgta
    10321 tgcaagtggt atcaaatact taattaagtt ttcttttgcc tctggcttta atagataata
    10381 tccactttta gatatcatta ctgggtccac aaccaaatat tttggattgt atttctttag
    10441 attctcaact atttcaagta taatctcagg acttgaaacc attcctattt ttacagctct
    10501 tggaggtata tcttcaaaga ctgcctctat ttgttttttt attattttct tatttaaatc
    10561 ctctacagca aaaactcctt tagtattctg tgctgttata gctgttatta cactcatacc
    10621 ataagtacca attgcactaa acgttttcaa atcagcctga atcccagctc ctccagatga
    10681 atcagagcca gctattgtaa gtgttggtat tttataatta ctcattgttg cctcctaaag
    10741 tttaatttaa ataacttaat taaaaaagct atacctacaa taggtatagc ttagaaatag
    10801 catatatcac aaataataca tacttgaatt tttactaagc tttcctacgc tggtattatc
    10861 cagatcaggt gtaagggtcg gtatacctct cagctcttgg ctcccctagc tattttaatt
    10921 tactatattt ataacttcat tatattacaa tagtatttat tgtcaagttt ttacactata
    10981 tgcacccatt ataatgttac tataaatatt tatgttaatt gttctatttc cttaccaata
    11041 atataatctt ctttattatc aattaaattt ataagtatac ttgggttttt tattatagac
    11101 aattttttat ttctagttaa tttttttata tagtattcta acttcatagc attgcttttt
    11161 gtgttggtca caaaataaac ttctagtttt tcaaatccct ttgctcttgt gtattttgaa
    11221 ttaattccta acttatgttc attcattctt ctaactatat tactggtata tccagtgtat
    11281 agagaatcat ctttacatct tataatatat gtaaaataca ccttaaatca catcctaaca
    11341 ttaaatatat aaatattata ttatagaatt aaaaaaaatg gtgtgtattt tgaatatgca
    11401 atcttcaaag aattaattat aatatatgta taaaacatac tctacatgaa tctaatgcta
    11461 aattttgaca taataataac atatttattt ctataataaa aaattcacaa attataaaaa
    11521 ataaaatttt caaaaaaatt ataattttca aaaaaacaaa ttgacacata taaaaaaatt
    11581 atgataccct aataaaaagg gggcaatgat tatggaaaac aatttaaata tagctaaaaa
    11641 aaataatact gaattattac aaaatgactc acttaaggtc aacttacaga taggtgtaga
    11701 ctttacactt ttatcaaaat cacttaaagc cttttataaa aaagaaaaag atttttattg
    11761 cattgctctt gcgccatgca atgtatctag tgaaaaagca aatagaaaaa taagtattta
    11821 cgatatgata aaagaaatta atattttaat tgaaagtata actgataaaa atgaactttt
    11881 aagtgaagaa ctctttacta ataatttatc agaattttat gatgaatctt tagatgagat
    11941 ttatatagac ttaaaacaag catatttata tattaaaaag aatacagata gtcttaacat
    12001 agaagactca attgaatatg tatttgatgt agatataagc aatgatataa aagcaaagga
    12061 aaatccacta attaatttta attcactttc tttagtagta tggagtggcg aaacaaaaca
    12121 tattttacaa caaatgaata ttttagactt agaggatatt cactcttatt atatggaaaa
    12181 taatacaaat ataactatct atgaaaataa tactcttcaa tataaatttg atgaagaatc
    12241 tgaagatata aaagacagta gcatagatat aattgaacaa atgcttatac ttcctgatga
    12301 cttgaattat gataaagatg aagttgaaaa tatgaaaagc agacttgcaa aaataaatat
    12361 taaatattta cagacactta aagaaaagga tataaaaata aaattaataa attcaaatct
    12421 aactgatgaa ccagaattta gtgatttaaa atatcaatta cctccatgtt gggtaagatc
    12481 aggcaagact tggaaagatg tacctggtat atatagaaat aattctatag tagctaaaat
    12541 aggttatagt aaccctagct atgcaaatgt acattcatca aaaaatttag aattacatga
    12601 aactgcacat gctatagata aaaatgtttt aaataaaaaa tcaaattctg aagaatttat
    12661 ggaagtattt gctcaggaaa gatataagct ttatgaccct aaacaagttg cacatgcata
    12721 tataagcaag tttatagaag aattttttgc agaatcattt gtgcattatt atcttgatga
    12781 agattcaaaa aatactttaa aggaaaattg tcctctaaca tatgattttc ttgaaaaact
    12841 tgaactaaac tattaagtgc gctaatatcc aataatacat ttaactatcc tcttttcatc
    12901 aacaataacc atataataat ttatactttg aataaatcac tatctataaa ggatttctta
    12961 gtatttatgt tataatattt ataatggtta tgttttaaaa tagtttatgt aacccataat
    13021 ttaaataaat aataaaagaa ggatgaaaag ttatgaagtt aaatagaaat gatagctgtt
    13081 ggtgtggaag tactaaaaaa tataaaaaat gtcatatgga tatggataat aaaatagatt
    13141 catttgctaa gttaggtcat attgtaccaa ctcatgatat tataaaaaat gaaagtcaaa
    13201 ttgaggaaat aaaaaaaagt gctgtaataa acacaaaaat tttggattat atttcagata
    13261 aaatcgaaat tggaatgtct acagaagata tgaataagtt ggttcatgac tttacactac
    13321 aaaatggagc tatccctgct ccactagact ataatggatt tcctaaaagt gtatgtacat
    13381 cagtaaataa tgaagtttgt catggtattc caagcaagga cattatctta aaagatggag
    13441 atattataaa tgtagatgtt tctactattt ttaatggata ctattcagat gcttcaagaa
    13501 tgtttatgat aggtaatgtt aatgaaaatg caaaaaaatt agttcaagtt acaaaggaat
    13561 gtgtccaaaa aggtcttgaa gttgccaaac catggggatt tttaggagat ataggtgatg
    13621 ctatcaactc ttatgcaaag aaaaatggat actctgttgt tagagaaata ggtggtcatg
    13681 gagttggatt aagctttcat gaagaccctt ttgtaagtta tgtatctaaa aaagggactg
    13741 agatgctatt ggttcctggg atgatattca caatagaacc tatggtaaat gaaggaactc
    13801 acgaaatttt tgttgatgaa gaaaataatt ggactattta tacaaaagat ggtaaattat
    13861 ctgctcaatg ggaatgtatg gttttagtta cagaaacagg ggttgaaata ctaactgaat
    13921 aaaagacaaa tgcctataaa aattgaaaat tcaattttta taggcattta ttttattaat
    13981 gcacatctat tgcatcaact ggacagcttt ctctagcttc tgctgctgat tcttcagcat
    14041 cacttggtac ttcatcaact ataacatgtg atttcccatc atccttcata tcaaatactt
    14101 ctggacatat tgatggacat aatccacaac ctatacaagt atcttgatta acatttgctt
    14161 tcataataaa atcctcctag acgctacaaa tttaaaatct tgtttatcaa agattgatga
    14221 accaaaactg taacattcag ttctttttta ttatgtcctc aaatttgaaa aaaatacctt
    14281 ttaaaataat ttttataaag tttattttat agttatataa atcctatata taatatacat
    14341 tatctaattc atcatactca taggacactt cttttttatt cttttttaca ggttctaggt
    14401 ttcttatttt tactattttt gctggtatac caacaactgt tgcaccatta ggtacatcct
    14461 taacgacaac agaatttgca ccaatttttg aatttgaacc tatatttaaa ggtcctagta
    14521 tttttgttcc agcaccaatt aatacatcat caccaacagt tggatgtctc tttccagtat
    14581 cttttcctgt agctcctaaa gtagctcctt gatatattgt caccctatta cctacttcag
    14641 ctgtctctcc aattacaaca cccattccat ggtctatcaa tataccttcc cccattttag
    14701 caccaggatg aatttctatg ccagtcataa atctggatat ctgggatatt aatcttgctg
    14761 taaaaagctt cttcttctta taaagagcat gagccatcct atgcattatc attgcatgaa
    14821 ctgatggata aagtaaaaaa acttctatct tagacctagc tgctggatca ttcttcatta
    14881 tatattcaat atctttattt attttcttaa acaaagtatt cactccctca aataaaatta
    14941 ttaaaatatt cccatagaca tatacttatc aactccatct ggagctactg taactatctt
    15001 tttatctgga tacttttttg ccatctctat agcaccaaat atatttgctc cagaagatat
    15061 ccctactaaa attccctctt ttgctgcaag taatttaact gtatcaaatg catcttcatc
    15121 atctactaat attacttcat caactacact agaatcataa ttttcaggaa caaagttagc
    15181 acctattcct tgtattttat gaggtccagt cttgttttct gatatagctg gagatttttt
    15241 tggttcaagt gcaattactc ttatgtttgg attttgttct tttaaatatc ttgcagttcc
    15301 tattaaagta ccacctgttc caactccaca tacgaatata tctaaatctt tgacatcatt
    15361 cataatttca actgctgttg ttttataatg tgcatttgga ttatctttat tttcaaattg
    15421 ttttaagctt ttatagtttg gattttcttt taaaagttca tttgcttttt ctatggaacc
    15481 tgccattcca gctttaccat ctgtaagcac caaatttgct ccataagcct tcattaaatc
    15541 tcttctctca acgctcattg tttcgggcat tacgattact acttcatatc cttttaattt
    15601 tcctatcata gataatgcaa taccagtatt tccactagtt gcttcaacta gtacatctcc
    15661 cttttttaat tctcctcttt gctctaagcc ctctaacatt tcataaacag ctctatcttt
    15721 tatacttcct gctggattta ctttttctaa ttttacatat atgttagaac acccattagt
    15781 acagcctaaa ttattcagcc ttactacagg tgtgtttcct atcaactcta atgcgttatt
    15841 atataacata ctatttcctc ctatatgtcc aaatctatta tcaaattgat aacgataaat
    15901 atattatagt attatcattt tgataatgca accttttttt ggattttata cttacaatca
    15961 ctttattcca aatattagta atattcaaat aaaaaagtga aaatcattat tgattttcac
    16021 tctttaaaat ttaaccttta aattaaatct tgagtaatat tttgttacta aaattccaca
    16081 aaatactata taagacctaa tatttttgcg acctctatat taacctttag tacattaact
    16141 ctatcttcac ctaaaactcc aaaaagtttt ttggaagtat tatcatctac aattttttgt
    16201 aatttacttt ctgatattcc agacgctttt gctatagctg gtatctgaat ttttgcagag
    16261 gctggactta tattagggtc aagtcctgaa cctgatgctg taagtaaatc tgttggtata
    16321 tcctctcttt tgacagttgg attatccttt aaaaactttt ctatatcttt ttgtactcta
    16381 tcatgaagtt ctggatttgt agctccataa ttaaaagacc ctgatgatac acctccataa
    16441 gatgcatttc catctttatc tggtactaaa tcttcttttg tataagtatt atagtttact
    16501 gatgatactc ttcccttaaa gaatctcgca tctgtaaagc tttgaccaat aagttctgaa
    16561 cctactttta caccatttac ttctatcata cttccattgg ctttatcttt aaatgcaact
    16621 tggcttacac ctgtcaaaac aagtggatat acaagtccac aaacaactaa caatacaata
    16681 cttacaagta atgctggctt taatgttttc atttttatat cctccattta ttaacctaaa
    16741 ttaagaatca ttactaaagg tgtaatcaat aaatcaatta attttattcc taaaaatggt
    16801 actataactc caccaagacc aaatataagc atatttctca ttaacatagc ttgtgatttc
    16861 ataggtctat attttactcc cttcattgca agaggtatta aacatgggat aattatagca
    16921 ttaaatatta aagctgataa tatcgcacta aatgatgtag aaagtttcat tacatttaat
    16981 atctccattt gaggtattgc aagagtaaat atagccggta taatcgcaaa atattttgca
    17041 acatcatttg ctatactaaa tgttgtcaaa gccccacgag ttattaaaag ctgttttcct
    17101 atttcaacaa cttccaatat cttagttggg tctgaatcta aatccaccat atttgctgct
    17161 tcttttgcag atgttgtacc actattcatt gcaagaccta catctgcctg agcaagtgca
    17221 ggagcatcat ttgttccatc acctgtcatt gcaactattt taccctctaa ttgttctttt
    17281 tttatagcat ctattttatc ttctggttta cattctgcaa taaatccatc tactccagct
    17341 tctcttgcaa ttgtagctgc tgtcaaggga ttatcccctg tacacattat tgtctttatt
    17401 cctatctctc gaagtctagc aaatctttct acaagccctg attttactgt atcttttaag
    17461 tagataattc catatatctc attatctaca cacactacta aaggtgttcc tcctagttta
    17521 gctactcgat ttacattttc ttctaaatct tctggcactg taccaccaga ttcttttact
    17581 ctttttatta tgctatcata agcacctttt cttatagctc taccatcagc taagttaata
    17641 ccactcattc tagtttgtgc agaaaattct acaaactcag cttcctctgc ctcttttgtc
    17701 ttacctgttg aacctaattt catacctaaa tcaactatag attttccttc tggtgtatca
    17761 tcttttaatg aacacataac agaataattt attaaatctt ttgaatctgc atttccaact
    17821 gctataaatt ctgatgcaag tctattccca taagtaatcg tacctgtctt atccaaaatc
    17881 atagtatcaa cgtctccaca agcttctact gcttttcctg acattgcaat tacattaaat
    17941 ctagttactc tatccatacc tgcaatacca atagctgaca acaatccacc tatagtagtt
    18001 ggtattaaac ataccaaaag agctatcata gtagaaattg gaatctttac acctgcatag
    18061 tttgccattg gataaagaga tattaaaaca accataaata taagtgttaa ccctactaac
    18121 aaagtattta aagcaatctc atttggcgtt ttttgtcttg aagcaccttc tacaagagct
    18181 atcattttat ctaagaacga ttctcctggt tttgttgtta ttcttatctt taaccagtca
    18241 cttacaactg tagtaccacc tgtaacagat gcgaaatctc cacctgattc tctcatgaca
    18301 ggtgcagatt ctccagttat tgctgactca tccacagaag ctatgccctc tataacttct
    18361 ccatcatttg ggataacttc tccaacattt actaaaacaa tatcaccaag ttttagctca
    18421 cttgcactta tcattttttc atttcctaac gtatctataa cccttgctac tgtatctttt
    18481 ctagttttct taagtgtatc tgcttgagct ttacctcttc cctcagctac agcttcagca
    18541 aaatttgcaa aaaggactgt tataaataag attattgtta caatcccatt ataagctctt
    18601 aaattattgc cttcatcacc aaacaaacta ggtactattg taagtatcaa tgttactata
    18661 aatccaattt ctacaacaaa cataactgga tttttcatca tatatttagg atttaatttt
    18721 ttaaaagctt ctataatgga agttttgaaa atatcttttg taataaactt tgttttttta
    18781 gtttccataa aactttactc cctcccaatt ttttagtgcc acaacactag atgctccgaa
    18841 ataggtccaa gcgctaaggc tgggaaaaat gttaaagcac caacaattaa aacaacaagt
    18901 actaaaacta ttgtaaatgt aaaattatcc gttctaagtg ttcctatact ttcatttaca
    18961 gcttttttag atgctaacaa acttgatatt gctagtagta ctataataga cacatatctt
    19021 ccaaagaaca taactatacc tgttgttata ttccaaaaca tagtgttatc tcctagacct
    19081 tcaaatcctg aaccattatt ggctgctgaa gatgcaaact catacaatac ctgtgataat
    19141 ccatggaatc caggatttga aattccttca agccctgctg gtattgctac tgaaagacca
    19201 gagaacatta gtattaaaag tggatgtaat ataataagta aagcaataag cttcatttct
    19261 tttccttcga ttttcttagt taaaaactct ggtgttcttc ctaccattaa tccacataag
    19321 aatactgcta ttatagcgta catcaacata ttcatcagac ctacgccttt accaccaaat
    19381 actacattta gcatcatatt taaaagtggt actaatcctc ctaaaggagt cagtgtatca
    19441 tgcatattat taactgtacc tgtcgtaaag gaagtagtca ctgttgtgaa taatgatgat
    19501 tgtgctatac caaatctaac ttccttacct tccatacttc ccatactttg attaagccct
    19561 aattgtgata aaatagggtt tcctgcactt tctgctttaa agcacactac tagacctatt
    19621 aaaaatatga ttgacattgc gccaaacaca acccaacctt gttttttatt ctttatcatg
    19681 tgaccgaatg caacaacaca agctcctggt aatatcatca tagataatat ttctactata
    19741 tttgatatta ttgttggatt ttcaaatggg tgtgatgaat ttgaaccaaa gaatccacca
    19801 ccatttgttc ctaaatgttt tattgattca agagctgcca caggacctct agctatatct
    19861 tgaagctttc cttctatagt agtcactgtt tctgtcccag caaaagtttg tggtactcct
    19921 tgtgtaacta atataagccc aataattaaa gctcctggta aaagtactct tgttgttatc
    19981 ctaattaaat ctacataaaa atttcccaat gttttctttt taccgactag acctcttaca
    20041 aatgccatag cagccgcata tcctgttgct gctgatgtaa acatcatata tattatcact
    20101 gtcatctgac taaagtatga aagtcctgat tcacctgaat aatgttgaag atttgtattt
    20161 gtcataaaac ttattattgt attaaatgat agtccttgtt ccatactttt aattccactt
    20221 ggattaaaaa tatgcatact ttgagttcta agtatgatat atcctataaa taccataact
    20281 gcatttgtaa ataataaagc taatgcatac tgtttccagt tcatttcttt tttcttatct
    20341 atactacata ccttatatat aaaattgtcc actttatcaa atagtctatc tccaaatgtt
    20401 ttttgattag tagctatatg atataaatac ttgcccatag gtattactaa aatcataaaa
    20461 atcgctaaca ctattactat ctgcaccata aaaattgttc cccccaattc aacttactta
    20521 ttagccttat actgtttaaa atttttcagg attaataagc gcatatacca aatatattat
    20581 taacattata attacaactg ccagaaacca cataggtaat ccctccatac attacattat
    20641 tttttctcag tttgtttccc acaccaatca gcaagtaact tcatggaaac aaatcctatg
    20701 aataacaatg ctatcataac tatatccaac attttagctc ctccatttaa tttaatctct
    20761 tttacaaatt ttagttttgt attgctactt catttatctt atcattttag gtataaagat
    20821 aggattaaga aacatccatt aatgattagt atttcataaa tataaaattg ggtttttaaa
    20881 atgcacatct agcaatcgta ttcccagtca ttccaacata attctatatt tgagatttgc
    20941 aaaaacctag atgggattat actttctata aagatttaga cacaaaaaag gtggcaaatc
    21001 tgccacccct aataaaatct aaagttattt aatttatatt aacattctgg aactatatca
    21061 ttacttatat ccatatccaa acttttacca tgataataaa taaaatcaat taccttgcca
    21121 ctaattgtaa cagcaatcaa agaataaaag atatcaaacg ctgatagata aaataatgat
    21181 aacaacaata caataacatc tgttgctaaa taaacttgcc ctatgttcaa agatgtagtc
    21241 ttagaaatta ctaatgctaa agcatcgtct cctcctgatg cacaacctgc ttttactact
    21301 atacctacac cagttccaac agttacacct gccaaaatag ttgataaaag catattttgt
    21361 gattgtggta ctagaggtcc tatagcttca aataagtcat ataacactga aaaagttata
    21421 gaagctacta ttgaatatat aaggaatttt ttaccgaaaa atttatatcc tactaaaagt
    21481 agtaaaccat ctattacaac atttgctatt gctggttgta tattgaataa attttttaac
    21541 agtagtaaaa tacctaacac tccaccttct gttatgttgt ttaaataata aaaattataa
    21601 actccaaatg ctaatattgc tgaacctaaa aatataagtc caatcctttc taaaaataac
    21661 cgcctggcca tacatatccc ttcctttttc tgaatttata tatattatat acaaaagaat
    21721 tggctttgta taccgaactt ttcaaatctt taattataca aaatttctat atttttttca
    21781 acaatttttt tatgtttttt tatgtttttt taataccaca cctacactat agtaattcca
    21841 atactttaga cgttttcctt ttttccataa gtttatatta gttttactta taatttagta
    21901 taattaagtc tttgtttata ttaatttttt atttgtttag aaatactatt caataattta
    21961 tcactaaatt aaattaaaaa aagagttact ttatatagtt tctaaagtaa ctctaaaaat
    22021 cttatttatt atctatgaca atttaccatt cacccttgaa ctctgaaata ttatctttgt
    22081 taacttcaac ttgtttaatt tgtgtaacag gttctatttt ttcaccttta aggaatttag
    22141 atgccttttc tatgactaat ccaccttctt ctacagcaga catataagtt gtaccatcta
    22201 ttaacccatc agttatagct tttaatccat ctttacttcc acccaaacct atgactttaa
    22261 ctttatcctt aagaccagca tcttttaaag cttgacctgc acctgtagca gaattatcat
    22321 cttgacatat tataacatta aaatcagttt tcttttgaat taaatcttct gttactttta
    22381 aagcttgctc tttctcccaa tttgaagttt gattataaac tatttctata ttactatctt
    22441 ttaattcttt ttcaagacct ttttttctta atacttgtgg agtagttcct ggtactcctt
    22501 ctattaatac agctttccca ccagtttctc ctaataagtt tttagcaatt tttccagtta
    22561 gtgctcctgt ttcctcctca ttttgtccta tatatgtaac taaaccttta aattctccag
    22621 ttgggttact tcctatagaa tttgtaaatg ctactatagg gacgtctgaa tcattagcag
    22681 ctttaactat tctctcactt acagcagaat caactccaca taatgcaatc atgtctaccc
    22741 ctttagccat aaaatcttca gcttgtgata attgtttact agcatcccat tgagcatctg
    22801 catattccac gcttacacct aattctttag attttgcatc tataccttgt ttagttgcta
    22861 caaagtaacc tgttgaacct ggtagtaata ctgctatttt cttttctcca tttttttctt
    22921 tgtcaccacc atttgaacaa ccaactaaac caaatatcat aacaagtgat aacaacaatg
    22981 ctaaatgttt cattaatttt ttcataaaaa aatccccctt attttttatc ttgaagcaag
    23041 ttttttcttg ctataagaat caaatccaac tgctgctaaa attattaatc ctttaaatat
    23101 gtactggaaa tatacatcta ttccaagaag attaaaactg ttgtttataa cactcataag
    23161 taatactcct ataacagttt taagtacaga accagaacct cctgccactg atgtacctcc
    23221 tattactaca gatgcaattg catccatatc atatccttca cctgcaatag cagttgcagt
    23281 cccaagtctt cctgttagta cgattccagc tattcctgca agtacagcat taataataaa
    23341 cactttcact ttgtgagaat ctacatttat accattaagt ttacttgctt cttgattccc
    23401 tcctacagaa taaacatatc taccaaactt agttttatta agtacaataa atgaaactaa
    23461 caccactata aagaaaatta ctactggtat tggtatatta aacaaatatc catttccaat
    23521 gtaatcaagt attggtatat tttcagtaat tggatatcct cctgtaagca tcaacagcaa
    23581 tcctcttaca atagtgacca tagccatagt tgctattaat gatggtacat ttatctttgc
    23641 aactactata ccattgatta atcctattac tgcacaaatc gctacaccta agagcatgga
    23701 tgcaaacaaa ttagttccac cagtcgcaaa tttcattgtt atagcacctg caagagctgc
    23761 tactgctcca actgatatat caaagtttcc agatattata acaaaagtca tacctgctga
    23821 aattattcca attatagaag attgtcgtaa aagattaagt atattttgtt tacctaaaaa
    23881 tgatggagtt gcaatactta tagctataca taaagctaaa aaacctaata atactccata
    23941 atctcttaaa tttaacttct caaaagcttt cttcatctca gtcctccatt ttcatttttt
    24001 atacattaaa tatactgttc attatatctt gaacctgtat attctcattt tttaaatcat
    24061 ctacaataga accttctcta gttactaaaa ttctatcact tactcttatt acttcttcta
    24121 aatcagaaga tataaatatt actcctattc cttcacttga aaattctttg attgtattga
    24181 aaatatcttc ttttgcagca atatctattc cctttgtcgg ctcatcatat attattaaat
    24241 ctaaatcttt ctctaaccac ttagaaacta ctactttttg ttgatttcca ccactaagct
    24301 cttttacttc ttgctttaca tcactaactt ttattcctaa ttccttaaca ccttttttag
    24361 aaaactcaat ttctttattt ctatctataa acaaaaattt cttgaattta tcaatttgta
    24421 ctgatgatga gtttgtgtat atagcttgtt ctaaaattaa tcctagattt tttctatctt
    24481 caggaatcaa tcctattttg ttttttatag caaccttagg agaagttatg tctacttttt
    24541 taccattgat aaaaatgtct ccactatctt tattatctat accaaatatc aaatttataa
    24601 tttcagttct tcttgaacct acaagaccag caagacctac aacttcgcct ctataaactt
    24661 taaatgaaac attttttatt ttgttagacc agcttatatt ctttacttct agtactggtg
    24721 gctcttcata ctttgcaaat gtataatcct tcttctcaat aagtttgtga ttatcatttc
    24781 ctgtcataag ttgagttatc tttaatttag ttaaatcctc tacatcataa gttccaatca
    24841 taattccatt tttcattata cttgcactat cacatacttt aaaaacttcc tctaatatgt
    24901 gagaaatata gactattgtt ttacctttag actttagtct acttattatt ttaaatagcc
    24961 catctttttc attattagta agtgatgttg taggttcatc cattatgata atttcagaat
    25021 tacatgaaac agctttcatt atctcaacca tttgttggtt tgctacactt aaattagaaa
    25081 ctactgtatg tgggtctata ttaaaatcaa aatcctcaca aattttcata tattcctcaa
    25141 gcatgtcttt cttgtttaaa aacacacttc cttttaaaat ttctctacca agaaaaatat
    25201 tttcaagtac tgtaagcgtt ggtatcaagc ttaattcttg ataaattata ctaataccat
    25261 tttctttaga atgatgtgaa tttttaaaca ttatttcatt accagtcaaa attactttac
    25321 ctttagtttg agaatgaact cctcctaaaa ttttaataag tgtagatttt cctgctccat
    25381 tagctcccaa aagagctctt acttctccct tttctaactc aaaatttatc ccttttaaaa
    25441 catctacctt tccaaaactt ttatatattt gttgcatttc taatatctta ctcactatat
    25501 cacctcacta attccgaaac cattttataa tttcatcaat attttctttt ggtgctgata
    25561 acaaatccca agataaaaca tatcctttta ctgttgtctt atttatattt gaaatatttt
    25621 cacttatgta cttaggtgta acaggagcta taatttcctt tttatttatt tcaactccta
    25681 catatatatc tttattaaga ttccttgtca taaaatcgat ttcctctagt acaaattcta
    25741 taggatatac atctccttca tactctttaa ttcctaaaat atctaagaaa tttctttttc
    25801 caatctcttt attcttatgt atggattcat caataaacct ttcaaattca aatggtaaac
    25861 cagctggagc ttttgtagtt ctatacatca ttggttttat aaaatctgct acttcttgta
    25921 atttttttaa atcttgacct gtaaagtaag aaataaaagg agaaaaagta tctataccta
    25981 ttttcaatcc cttactttta aaataactac ttatctctaa taatgacttg tatatatttt
    26041 gattttttgt ttcaaaaaaa tcttcccata ttttattttt aaatttgtat tttccattaa
    26101 tgtattctgt tataccagat ggagtatctt cattatctat aactccctta gacacattat
    26161 tcatttctct cattaacgac tttttatcaa tattattttt ttcatatata tttaaacaat
    26221 ctttacaaaa acaattaaaa acaccattta gtttattcga aaaagaacca taccttattt
    26281 tatctaaaaa aattccatca aatttaatat cactaaaata agtactatat attttcttta
    26341 tattttttat attttcctta ttatttgggc aataaaattc aaaattttca tcctcttgta
    26401 aagcatagcc cttgacttcc ttccccatat aatcaattag taaattactt ttttcaagta
    26461 atcctatttc agaaaaacat ggcaaccaaa gatatagttc aacattatat ttatttaata
    26521 gttttttagt ttctacatat aattcttcat ctacactcca tcccatgata accttttcta
    26581 tatcaataca gtccaataat tctagtagtt tacattttat atcattaaac tttatttctt
    26641 tagccttaaa acctccagta aaaatttgta ctattacctt ttttctatcc atgcttaact
    26701 ctcctattag ctaatgccat agcaagtctt atccccccat atactggctg attttcttga
    26761 gatataaatt ttaaattttt atctgtattt tctgttttta aattattcat taacatttga
    26821 gcatatacag ttttatttaa tgtaccacca gacaaaacta tatttatttc gcttttatct
    26881 attttcattg gattagatat ttttctcaat attacttgag ctaatttaga tagttcagtt
    26941 gatgattctc ttaatatttc aatagctaat ttatcacctt gttcggcaca atcaatcact
    27001 aatgaagcta ggtttgctat ttcaacatta ttgtttatat cagtaatcat atatgcaagt
    27061 tgttcaaaac tatttgctcc aaagaaatcc aagacacaac taaataaatc actatactca
    27121 tgacattcat cacaataaag caatactttc tttaataatt ttctccctat atcatatcca
    27181 gaacccaaat cactaaaatt atacccccat ccaccaactc tatatatttc tccatcttct
    27241 ttaattccca gtataattga acctgtccca gccacaatta ctaaaccagg ggaatcagct
    27301 tgtgcataaa aagctaaaac tgcatcattt gccagatata ttttttcttt gttaatccca
    27361 atttttaata tttcatccat tataatctta taatcattag gagaatcaca accagatata
    27421 ccaaatacag tgtactcaat atcgtcataa tcgatatata aatcttttaa tattttttta
    27481 agtccattat taatattttc atatgttttt ttaattccaa ctgacttata attagttgaa
    27541 ccagtggtat gttctttaag tatgtttcca tctaagtcac ttacacaaaa tttagttttt
    27601 gttccccctc catctattga aaccaaatat ttcacattaa tatcctcctt ttttattaga
    27661 taaggaatat tgatagtcag tttcttttaa acacatacta attactccat ttatacttct
    27721 ttctctcaat gactctactt ttattaattt aggaaaattt tcttctccaa cattattact
    27781 tacatctttt tctataaact ccatgtcact cttggttaat atactacact gtataatcat
    27841 agcttgtgga tttaggacac atacaagagt ccttataaag tttgatatta tagataaaat
    27901 ttctttctta aatttattat ctttactttc taaatatctt tttcttaaac tcataatata
    27961 atcttcaaga tttaagttgt tagagttata cttaccaaat tctatattta gataacttaa
    28021 ttctccagca aaatttgtag aacctaaata caattcacca tttataacta tacctgaacc
    28081 aataccatct tctaaataca taagagccat atttttatat ttgtctttat actgtttttg
    28141 ataaatccca atagtagtaa tatttgtatc attttctata aacactttag tattgtactt
    28201 actctttatt aaattttcta aatttatacc ttcccaactt tttatagttg gtatattttt
    28261 aactacacct ttttttacaa ctcctgggac accaattcca atagccctta tattgtttcc
    28321 tttttccttt tttattagag tatctataac aaaaaaagta tcttggttaa cgttttcata
    28381 tgtatccatt cttatattaa attcttcttg ataaattatt tcacctaata aattggcaac
    28441 tacccctata caacaatttc tataataata taaagctatt atgtgttcta agtttgcatt
    28501 tatttcaaat aattgagctc ttcttcctcc agtagattca cttagtccac ttactttaac
    28561 ttcatttttc aaagctaatt gctccacaat cttattaact gtagctaaac tcaattcagt
    28621 aatgcttgaa atttctggtt ttgttatagg accttcattt tttataatgt tttttattaa
    28681 atcttcattt acttttttta tcgtttgcgg ttttccaact atatttttca tagacaacac
    28741 cttactttat aaactttatt tattaagttt agtataatat ttacaagtta aatttgtcaa
    28801 taaattttca ataaaacttt aaaatcaaat ttctcaattg tctgcttcat aattattctt
    28861 cctgactaag ttttattgtt catattcctt tcaactgttc atattagttt gatgatataa
    28921 atatatatta tagtattgac tataataaaa tatcctcata caaagacagt tgcaattatc
    28981 tatctgaata tatataataa aaatgtaaat ttgtcattga actctatctt aaaatcgaaa
    29041 tcaatgttca gatgaatttt tcaaaccaga aaggagtatt tagtttatgg gaattggaac
    29101 ttgtgcatag caaaggctat tgtacataat aaaaaatttt atttttaatt gtgagtagca
    29161 gtagcctttg ctaatcctaa agagtaatta tttaggaggt gcaaaatgag ctatcaaaat
    29221 tcgattgact tactgcctaa agaactgatt gagcaagtgc aagaatatat agatggtaaa
    29281 gtgatttata ttcccaaaaa gcaagagcac aaaaagcatt ggggtgaaaa cacaaacacc
    29341 aaacaagtct tagcttctcg taattctcaa atctgtataa atttccaaaa tggaatgagt
    29401 atcaagcagt tatcagagaa atactttttg acagagaaaa gcatacaaag aatacttaaa
    29461 cagcaaaatt tataattaga aatgtgtcac gttttatgcg tggcacattt ttattctaag
    29521 aaagaacatg aggtttttca ttcataccta tattgttata attaacttaa caaataaaaa
    29581 ggaggaaaat atatgtcata ttttcagtat caaggtaatt cttgttttta taaagaatat
    29641 ggacaaggta aaccgataat tttcctgcat ggtaatactg gttcttctaa tatgtttaaa
    29701 gcacttgtgc cattatatgt agaaaatttt agatgtatct taattgattt tttaggtaat
    29761 ggacagtctg atagagtttc tcaattttct cctgacatat ggcatgatga agccttacag
    29821 acaatagcac tgattgaaca tttgaattgt ggtaaagttg gcttaattgg aacaagtgga
    29881 ggagcatggg cagctgttaa tactggatta gaacgtcccg atttagttga agcaattata
    29941 gcagatagct ttgatggacg tactcttaat gataacttca tagataatct aatcactgga
    30001 agggaaaaat caaagcaaga tattcaagca cgaaaattct atgaatggtg tcatggaaaa
    30061 gattgggaga atgttgttaa tcttgataca aaagctctat tgcaatgtgc aaacaagaaa
    30121 cgccctttat ttcataaaga attgtgtaaa ttagaaatgc caattttatt tacaggaagt
    30181 aaggaagacg aaatgtgtcg tcataatctt gaagaagagt acaagcaaat ggcaactttt
    30241 atttcaaaag caagtattca catttttagt cagggaggac accctgctat attgacaaat
    30301 gcagatgaat ttgcacagtt agcaaaagtt tttttcaagt attaactaaa aatattaact
    30361 atttcataaa cattatttag aaaagaaata ttaagttagc atttaaacac acaggtagta
    30421 ttatcctatt atatacagat ataaaagaaa cgccgaggcc cacaaaatat agtatttatt
    30481 caggttctca gcgttttctt tatctcagtc taatactaaa tctcgactgc caaaataaaa
    30541 ttttttacta ctttttaaac ctaacctcag cagcctttgc atgtgcatat aatccctctt
    30601 cttttgccag cttttcagct acaggtgcta atgattgtat agctttttca ttaaatatct
    30661 gctttgtaca agttttgata aaagttccaa cccaaacacc accagtatat cttgatgctt
    30721 taagtgttgg taatgtatga ttggtacctg aaacatagtc accaaacacc tcagcagaaa
    30781 gatttccaat aaataaagaa ccatagttag ttaatctatc acaaatatca ttatactcat
    30841 ttactgcaac ttctaaatgt tctggtgcat aaaaattact tattttaatg gcttcttcca
    30901 tatcatcaac caatattatc tctccattat tttcccatga actatatgct atgtcctttg
    30961 ttggcaaatc ttttagcata ctttctatat ttttttcaac tttttgtgct acttccaagc
    31021 tatttgtcaa taatattcct cttgcattta aatcatgctc acattgtgct aataaatcag
    31081 ctgcaatata aactggattt gaagtttcat ccgctattat aagaacttca cttggcccag
    31141 cgacaaaatc aattcctact tgtccataac attgtctttt agcttcagtt acaaacttat
    31201 ttcctggtcc tacaattatg tcaactggat taatcttttc tgttccatat gtaaatgctg
    31261 ctatagcttg aactcctcca gttgcatata tttcatcagc acctgctata tccatagcta
    31321 ccaatgtttt aggatttatc ttttcagtat ttttcatagt tggtgaacat gccactattc
    31381 tttttactcc tgctactttt gcaggtatta taagcataag agctgttgaa aacaatggat
    31441 actgacctcc tggaacatat gcaagacatg actctattgg aatattagtt tgacctaaga
    31501 ttacacctgg ataaatttct ttttcaaaag ggttttcaaa acttagtttt tgagctttag
    31561 caaattctct tatatttttg gcagcaatct ttaagttatt tataaattcg tcatctacat
    31621 gcttataagc ttcctttatt tcttccttag taattctaaa ttcatctctt gtatttctat
    31681 caaacttctc attatatttt ttaagtgcaa catccttatt atttcttata tcttctatta
    31741 tagaacttac ttgctctctt aatatctttt catctacctt ttctttcata cttgatgttt
    31801 taatactttt cattttttac ctcctaattt tacaatctca tactcattta ttttacaata
    31861 ttatcctcat ttaagatata tcaataaata aaaactccca ccctctatca aaagctagag
    31921 gatgggagtt taatcatatt gtactcacct cactaactga gcactatttg tctaataaat
    31981 atacttaaat ttattctaat cattattttt ttctgaattt tctctttatt atacataata
    32041 tgatatattt taactattga tttgtcaatg aattttttta ttttctacag cagttacaat
    32101 tacatttatg gcatttatgg caacaattat tattgtttcc tccattattt ccatttcttc
    32161 tagcttcaca ttccatacct tcatcaaata atctagcagc ttctgctaaa tattcgattc
    32221 ctttatagtt gtattttgaa tattcatccc agtatttttc tgcttcttcc cataattttc
    32281 tagcttcaca ttcatatgct agtgcttctt tctttctctc tgaagattca cagaatgctt
    32341 ctcttgcttc tctttctctt atctcagctt ctcttctaaa tctttcacag tttctaggtc
    32401 tatttcctcc tgaatctgaa catccacagt ttgagcctct tctttctcta tcttcatcgt
    32461 atgctgctct ttcatattct tctctatcat tctggaacca tttagctgta gattctcctc
    32521 tttcatacca gtcttctgaa taaccaccat ttgcatattt tttattttcc atataacata
    32581 tctctccctt gaattttaat tttttatgag atgttttgtt tcatctcata ctatatagta
    32641 ttaaaatcgt caaaatatgt tacatataaa cttatttttt cttttttaac ttattttcaa
    32701 cattttattc attttttcgc aatatccttt atcttttatt agacataaac tgttgtttat
    32761 ttcatattca gaatcagcat aaaccataat agcaagttca tcatcatcat ttaaaatatc
    32821 gaattttact aaacaattct caactatatt tagaatttca tctttattac attttttaaa
    32881 tctaagaagt gctccacact tttctctaac tattttatta acttgtattt tatctttagt
    32941 aaaagaatct atacttgaaa ctctattatc tattatatct ataacatctg aataaaccgc
    33001 acttcctgtt gctaaacttc cagctccttt acctacaaaa gaaacttctc ctactgaatc
    33061 tccattcact atgacagcat tgaactcatt gtctattcta gcaaggatat tatctttttc
    33121 tactaaaaca ggtctaacaa aagcacttac tttatcattt tcatacttac tttgaccaat
    33181 caattttatt ttacaattta atttttttgc atattcaata tctttttcat ctatatctgt
    33241 aattccttct aaatacacat ccttccaata tactctattg tcataagcta gagttgataa
    33301 tattgatagt tttctagcag catcataccc taatacatct gactctggat ttgcttcagc
    33361 aaaacctaat tcttgagctt gtcttaatgc catatcataa gatagattct catcatacat
    33421 tttagataat ataaaattag tagttccatt taaaattgca tttatactat ctatattatt
    33481 tccttctagt gattctatta ttggttttaa tactggaata cctcctgcga ctgaagcttc
    33541 aaatttaata cttactttat tttcacttgc taattttgca agctcatctc cacattcagc
    33601 taacaaatct ttatttgctg taacaacatg aattttattg tttaatgctt tttttatata
    33661 agataatgat ggttctaaac cacccatgac ttcaacaagt atatctatat ctttatta
//
LOCUS       DAMKPG010000035        33416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMKPG010000035 DAMKPG010000000
VERSION     DAMKPG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.038"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            257..1123
                     /locus_tag="PC621_001789"
     CDS             257..1123
                     /locus_tag="PC621_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF4108948.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1365..2174
                     /locus_tag="PC621_001790"
     CDS             1365..2174
                     /locus_tag="PC621_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108949.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2317..3381
                     /locus_tag="PC621_001791"
     CDS             2317..3381
                     /locus_tag="PC621_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF4108950.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3409..4128
                     /locus_tag="PC621_001792"
     CDS             3409..4128
                     /locus_tag="PC621_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4108951.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4152..4736
                     /locus_tag="PC621_001793"
     CDS             4152..4736
                     /locus_tag="PC621_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF4108952.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            4738..6087
                     /gene="pabB"
                     /locus_tag="PC621_001794"
     CDS             4738..6087
                     /gene="pabB"
                     /locus_tag="PC621_001794"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF4108953.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6077..6820
                     /locus_tag="PC621_001795"
     CDS             6077..6820
                     /locus_tag="PC621_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF4108954.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            6854..7555
                     /locus_tag="PC621_001796"
     CDS             6854..7555
                     /locus_tag="PC621_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108955.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7581..8153
                     /gene="folE"
                     /locus_tag="PC621_001797"
     CDS             7581..8153
                     /gene="folE"
                     /locus_tag="PC621_001797"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF4108956.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8219..9010
                     /gene="folP"
                     /locus_tag="PC621_001798"
     CDS             8219..9010
                     /gene="folP"
                     /locus_tag="PC621_001798"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF4108957.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9021..9386
                     /gene="folB"
                     /locus_tag="PC621_001799"
     CDS             9021..9386
                     /gene="folB"
                     /locus_tag="PC621_001799"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF4108958.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9379..9885
                     /gene="folK"
                     /locus_tag="PC621_001800"
     CDS             9379..9885
                     /gene="folK"
                     /locus_tag="PC621_001800"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF4108959.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            9866..10651
                     /gene="aroF"
                     /locus_tag="PC621_001801"
     CDS             9866..10651
                     /gene="aroF"
                     /locus_tag="PC621_001801"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF4108960.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            10906..12696
                     /locus_tag="PC621_001802"
     CDS             10906..12696
                     /locus_tag="PC621_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF4108961.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12714..13880
                     /gene="rpoD"
                     /locus_tag="PC621_001803"
     CDS             12714..13880
                     /gene="rpoD"
                     /locus_tag="PC621_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF4108962.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14045..14734
                     /locus_tag="PC621_001804"
     CDS             14045..14734
                     /locus_tag="PC621_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF4108963.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14748..15845
                     /locus_tag="PC621_001805"
     CDS             14748..15845
                     /locus_tag="PC621_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF4108964.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            15907..16479
                     /locus_tag="PC621_001806"
     CDS             15907..16479
                     /locus_tag="PC621_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF4108965.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16539..17006)
                     /locus_tag="PC621_001807"
     CDS             complement(16539..17006)
                     /locus_tag="PC621_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF4108966.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(17392..17868)
                     /locus_tag="PC621_001808"
     CDS             complement(17392..17868)
                     /locus_tag="PC621_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF4108967.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(17988..19079)
                     /locus_tag="PC621_001809"
     CDS             complement(17988..19079)
                     /locus_tag="PC621_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF4108968.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(19072..19461)
                     /locus_tag="PC621_001810"
     CDS             complement(19072..19461)
                     /locus_tag="PC621_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4108969.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            19653..20099
                     /locus_tag="PC621_001811"
     CDS             19653..20099
                     /locus_tag="PC621_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108970.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20363..21088
                     /locus_tag="PC621_001812"
     CDS             20363..21088
                     /locus_tag="PC621_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108971.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            21075..22319
                     /locus_tag="PC621_001813"
     CDS             21075..22319
                     /locus_tag="PC621_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4108972.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            22399..23076
                     /locus_tag="PC621_001814"
     CDS             22399..23076
                     /locus_tag="PC621_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4108973.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23077..25638
                     /locus_tag="PC621_001815"
     CDS             23077..25638
                     /locus_tag="PC621_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4108974.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            25859..27133
                     /locus_tag="PC621_001816"
     CDS             25859..27133
                     /locus_tag="PC621_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF4108975.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(27294..30335)
                     /locus_tag="PC621_001817"
     CDS             complement(27294..30335)
                     /locus_tag="PC621_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF4108976.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            30542..30841
                     /locus_tag="PC621_001818"
     CDS             30542..30841
                     /locus_tag="PC621_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF4108977.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QDLINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(31079..33307)
                     /locus_tag="PC621_001819"
     CDS             complement(31079..33307)
                     /locus_tag="PC621_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF4108978.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        13198 a         3669 c         5621 g        10928 t
ORIGIN      
        1 gcagtatcat attatgctag aaaaagtttc atttaaggca taaatgagat gatttgaacg
       61 tttttaaaga ttagaatata aaagattgca ctagacatat atgaaaaaga taatataata
      121 gatgatgtaa tagatgacag ttcaagaaat tataaaaaaa atttatttga aatattatct
      181 attacatcag gaataatata tgtaagagta agcttttaaa attctaaaga tttagaaaga
      241 ttgaagggag aaagaaatga gtgaagttaa gaatacccta caaaaacatc cagttttaag
      301 atattcaaca atgctatttg ggatgttgat gacatctata ggaataaatg ggttcttaag
      361 accagcacat ctgcttagtg gaggtgctac aggtatagca acatctataa actatttgac
      421 aaatataaat gtaggtttat taacattttt aatcaatatt ccaatattta tcttagggtt
      481 catatacctt gaaaaagagt tttgtatttc aagtttggta aatatgattg tattttcatt
      541 attattagga gcaactcagg aaataagtaa tattatacct atacatgata tattgcttca
      601 aagtgtatat ggtggtatat tatctggttt aggagtcgga gttgtattta gaactaggtc
      661 ttctcaaggt ggtacagata taatagctgc aatactaaaa attaagaaga atatagaaat
      721 gaaagacact gctttagcaa taaatggctt gatagtactt acaggaagtt ttttatttgg
      781 gttggattta gcattataca ctcttatagg tctatttcta aatgcgtatt caatgagttt
      841 tattaaagat gcaatgaatt accaaaaatc tgttatggta atgtcaaatg aagtcgattt
      901 aatagcagaa gatataatga agagtttagt tagaggtgta acttttttag atgcagaagg
      961 tgcatacact catcaaaaaa agaagattat atatacaata gtttcatcaa atgagatacc
     1021 aaagataaaa gatatagctt tgaagtatga taaaaaggcc tttatatctg taaatgatgt
     1081 tacagaagta aaaggaagag ggtttaaagc aaaagattta tagaaaattt tacaagctgg
     1141 gagtttataa tgtaaatttg tatattatat ctctcagctt atttaaattt agtatatgaa
     1201 aataaaggtt ttatcaatga tttgtgtatc actttaaaag atatcatttt aaaattaaaa
     1261 ttgaaaaaat tactcaatat gtagaaaaca taaccactat taggatataa taaatttaat
     1321 agatttagta aaatatctag gaataacgtt ggaggaatag cattatgaat ttatctaaag
     1381 aatatgttaa tttgtatgat aattatttga aatatataaa tgaagtaaac aaagagatta
     1441 gaagtataaa gaacatgaag aaacgtgttt taaaaaaggg cgattttgct ccagaaaatg
     1501 aagtattaga attggaagga aaaacaatac gcgatataga tgatgtcgat acaaaaaaac
     1561 caaaaaataa aatatataaa ttttcaaatg gggatgtgta tgtaggaaaa tttttagaag
     1621 gaaagatgga agggcaagga tcatatactt tttttgttga tgaaggaacg gctatggagt
     1681 acataggaga gtttaaagaa gacaaaaaaa acggaaaagg aaactttgta ttttcaaatg
     1741 gaaatgaata tataggccat tttgaggaag atatgatgaa cggtattgga aatatgttat
     1801 ataatagcaa agatgaatat attggaactt ggaaaaatgg aaaaaaagat ggaaaaggta
     1861 tttataagtg gagtgatgga tgtatatatg caggtgaatt taaaggtgga aagatggaag
     1921 ggtatggtat ttgctataat agtaaagggg aagtacttta tgaaggagaa tggaagaata
     1981 atttgattca tggtaaagga acttatatat gggaaaaagg taaaaagtat ataggtgaat
     2041 ttatgcatgg taaaaaacat ggacaaggaa ctttttatct aaataatgaa ttggtttatg
     2101 aagggacttg gaaatttgat aagccatcca tcttcgacag aacattagat gaaatatttt
     2161 cattaagact atagctttat ctattaatag ataaagcttt tttctaatac ttactgtaaa
     2221 taagtatgaa tgatattgat tagtatacac tatcaatatt aactcaataa atgtgatatt
     2281 aaaaattttt gattgatttt aagaaggagg gatatattga agagattaat tgctttagtg
     2341 ttagtaatac ttattgtttt attttatagc cctgaattga tagaagaagg atatgattta
     2401 aaagaaaaat tttttcaaag tgaaatatgg aatgaatttt tagacaaaat aaatataaat
     2461 aataattcta agaaagataa aaaagaacaa aatcaaaatt tacaatcaaa taatactgaa
     2521 ggggagaact taggagagtc agatataaag aattttgaaa aaataagctt aggagataat
     2581 ttaagctatg ttttatcaag tattggtaaa cctggtagaa ttgatattag tgaatatggt
     2641 tttgattggt atgtatataa tcaatatgga aaagaatttg caatggtcgg acttgaaaat
     2701 gatgaagtag tagcacttta tagtaattct attaattcat gtgaaaatca agatataaaa
     2761 ctaaatcaag atagacaaac agtgagaact aagattacac cattaaaata taagagaaaa
     2821 ggaaatacca gatatataat aaactcagaa aatcaatatg atataataag taaagaaggt
     2881 aagtatatta caattttcta cgatattcat gaagaaaata gagtttgctc atatttaata
     2941 attgataaaa gtactgaaga tgagtttaaa aaattatatc cagatgatag agaagaactt
     3001 aaaaaatgtt ttgaacttga agttatagac cttgtaaata gtgttagaaa tcaaagagga
     3061 ttaaactcat tgagatacag tgaacaggca actttgagtt caagaaaaca tagtgaagat
     3121 atgagagata ataatttttt tgaccatata aataagaaga atgaaactcc atttgacaga
     3181 atgaaaagag aaggtatagt atatacttca gctggagaaa acatagcagc aggacagatt
     3241 aatgcaatat atgcgcatga agcttggatg aactctgaag gacatagaaa aaatattttg
     3301 gggaactata ataatattgg agttggagta atatttggag gaagctataa aacatattac
     3361 actcaaaatt tttataaata actaaaatat ttgaaaggaa gttaaaaaat gacatcatgg
     3421 aaaaaaaaga cagtatataa gtgcttaata gcagtagtct tgttttgtgg aatagtgtta
     3481 atatctaatt ttagtaaagt ttctgctttg atgatggata ctaatggaaa tgttttgatt
     3541 aaacatggtt caagggaaaa aaagctaata gcaattacct ttgatgatgg tcctcatcct
     3601 aaagaaacta gtcaagtttt agatgtatta aagaaataca atgtaaaagc tacttttttt
     3661 atagctggaa aacatgcaaa atggtataaa gaaccattag taagagcaag taaagaaggt
     3721 cacgagatag gaaatcatac tttcaatcat cctgatataa gtaatttaag ttctagtcag
     3781 atagaagagg agattgttaa gtgtgaagac attttgaaag aagtaactgg aaaaaaacct
     3841 acattattta gaccaccatt tggaagttat agagaaaaag acttaataga aattgcaaaa
     3901 aagcatgact ataaagttgt tttatggaca ggtgtagatg tgaaagattg gaaaaatcca
     3961 ggagctaata gcattgcaga taaaataatt aataaagtaa aaaatggaga tataatactt
     4021 ttgcatgatt atgcaactaa tgatacagtt gaagctttag atatgtttat tccaaaaatg
     4081 attgaaaaag gttttaaatt tgtaacagtt tctgagttga taaaataatt tttagggggg
     4141 agatttacag tatgattctt atgatagaca attatgattc ttttgtatac aacctagtcc
     4201 aatatataga agaattaggg gaagcagttg tagtaaaaag aaataatgaa atcaaaataa
     4261 gtgacattga agaactaaat ccagaggtaa ttgtgttatc tccaggaccg tgttctccaa
     4321 aagaagctgg tatttgcata gatattgttg agcattttaa ggggaaaaaa cctatactag
     4381 gaatttgttt aggtcaccaa actattggat atgtttttgg aggagatatt ataaaagcac
     4441 aacagcctgt gcatggaaaa gtatatagta tcaaccatac taataaagga gtttttaggg
     4501 gacttaaaaa tcctttaaat gtcactaggt atcattcact aataattgat tccaatacag
     4561 ttccaaaaga attagaaatt acagcaatta ctgataaagg ggaaataatg gggattaggc
     4621 ataagaaata tttaatagaa ggagtacagt ttcatccaga agctattttg tcagagtatg
     4681 gtcatgaaat gcttgaaaat tttataacag aagctagaga aagggtacat atgtaatatg
     4741 ataagagaga taaatactaa attaaattct tttgagatat ttactatttt tagaaatgaa
     4801 catgacagtt ttatattaga cagtgctatg gataaagaaa aattgggaag atattctttt
     4861 ataagtagcc aaccatttaa ggtattaaag tataaggata ctgatgaaaa tcctttagaa
     4921 gttttaaaag aagaactaca taagtatagg gttgtaaatg atactaatct tccatttgta
     4981 ggaggtgctg tgggatattt atcttatgat ttaggtaatt acatagaaaa tttacctaga
     5041 actgctgtag atgatattga gatgcctgat atgtattttg gattttataa tcatgtaata
     5101 gttatagacc atcttgtgca aaaaacttat atagctactc caaacataga tatcgaatta
     5161 gaagaaaaga taatagatga tatagaacaa agaatattaa aagaagagaa aaaaggtata
     5221 gatagcatat gttatgaaga aaaagaagta acacctataa gacttaaatc taattttaca
     5281 aaggaagaat ttaaaaatgc agttcaaagt gttagagagt acataagaca aggagatata
     5341 tatcaagcta atttaacaca aaggttcagt ggagaaacag aacttacaag ctttgaattg
     5401 tatagggact taagaagatt tagcccagca ccatttgggg cattcttaaa ctttgaagat
     5461 acacatatat tatcaaattc tccagaaaga tttataagat gtgttaataa gagaatagaa
     5521 actagaccaa taaaaggtac tcgtccaaga gggaaagata aagaagaaga tttaagactt
     5581 caacaagaac ttagaaatag tgaaaaagat agagctgaac tacttatgat agtagattta
     5641 gaaagaaatg atattggcag aatatctaaa actggaagtg ttaaagttcc agagctattt
     5701 gtaattgaac catatgcaaa tgtaaatcac ttagtttcca cagttgtagg tgagttaaaa
     5761 gatgataaag atgctactga tgtaataaaa gcaacttttc caggggggtc tataacagga
     5821 gcacctaaga ttagagcaat ggaaattata gacgaattag agccaacgca aagaaatgta
     5881 tatactggtt caataggtta tataggtttt aatggtgata tggattttaa tatagcaatt
     5941 agaacaataa tcaaaaatga caaaaaagtc tattttcaag ttggaggagg aatgacttgg
     6001 gattctgacc cagatgaaga atatcaagaa acacttgata aagctaaatc cataatgaag
     6061 gctttgaggg gatattatga agaataatgt aggttttgat agtagtttaa gtaaatttgg
     6121 aattggtttg tttgaaacta ttagagttaa aaatggaata gcagtagatt tagatatcca
     6181 tatagaaaga atgctaagtt caataaattg tttggattta gatataaact ataaaaaaga
     6241 tttcttgata aatgaaattg ttacatatat aaaaaaagaa aatgtcatta ataaagcact
     6301 tagaattact gtttttgatg aaggttataa tatatctatt agggatattc catataatca
     6361 agagacgtat gagaaggggt ttaaattagc tatatcgcca atagttaggg ggaatagttt
     6421 aattcataga cataagacta ctaattattt tgaaaacata tataccaaaa atatcgcaaa
     6481 taagactggt tataatgatg gtatattttt aaattcagaa ggtgtaatat tagaatgttc
     6541 tatgagcaat attttcttta taaaaggaga aagagtgtac actcctagtg atagattacc
     6601 gatattaaat ggaataataa aaaaaaggat tatagagatt tgtgacgaat tacatataga
     6661 gttgatagaa aatgaaatta atatatcaga aattagtagt tttgattttg tttttgtcac
     6721 aaatagtctt atgggcgcta taaaggttac agaaattgac aaaataaaat ttaataaaga
     6781 aaatgttgtt tttgataaaa tagtgaagtt attagaataa attaaaatat taacaatttg
     6841 ttggagacaa aagatgatag gttgtattta tgaagtatac aatgaagaga tgactaaaat
     6901 aatagaaaac caaaacactt tggctgtata tttagtaggt tctagtaaag acgtagattt
     6961 tactctttat gatgtagaga taaacgatat tgatgtgttc gtttttgtaa aagaagggga
     7021 aaaacaagag agaatactgt ttaaaaaaaa gggaatagaa tttgatgtaa attacttttc
     7081 aaaagatggt gtaaaaaaat tattatcaaa tagggaatat ttctttgtga aagaaatgaa
     7141 agatgctaaa gtactgtttg atagggagaa tatatcaaat attataaaag atataagtag
     7201 agatatttac ttagaaggac ccagtaaaat gtcattagag gaaaagtctt ttataaagca
     7261 agatattgga gcaaaaatat cgaatttaaa aaataaagaa aaatttgatg tttttgaata
     7321 tcattttttg acgaatttat atttaaaaga tataataatt gggtacttta atataaatga
     7381 taaatgggta ccaaaagaca aaaaattgct aaaagtttta aaaaaagaaa ataatgagct
     7441 ttttgaacta gcgtcaaaag taagtgaaaa ttatgactat caaagacttt tagatgttta
     7501 taattatatt tttaaagaaa ttgagacaaa agaggttata aaactaatct tctagtcaca
     7561 aatcaatggg gggagtttaa atgaataaag ttgataaaga aaagatacaa catgcagtaa
     7621 gagaaatact agaggcaatt ggagaagacc cagatagaga aggtctaatc gagactccaa
     7681 atagagttgc taggatgtat gaagagatat tctctggtct tagtgaagaa ccaagagacc
     7741 atttaaaagt tctttttgca gatgaaaaac atgaagaatt agtattggta aaagatattc
     7801 cattttattc atgttgtgag catcatctag ttcctttctt tggaaaagct catattgctt
     7861 atttaccaaa aggaggaaga cttacagggc tttctaaact tgctagagta atagatacat
     7921 tggcaaaaag accacaatta caagaaagaa tcactaaaaa tgctgcagat ataataatgg
     7981 aagaattaca accttatggt gtcttagtag tagtagaagc tgaacatatg tgtatgacaa
     8041 tgagaggtgt aaaaaaacca ggttcaaaaa ctgttacatc agcagtgaga ggaatatttg
     8101 aaaaggacat tgcgtctaga gccgaggcta tgagtttgat tactatgaag tagcaattta
     8161 ttatagatat aatcatttat tgagatataa aatttaattt aagggagtgt cattaattgt
     8221 gtttgattat ggtaaaagaa cttatataat gggaatatta aatgtaactc cagatagttt
     8281 ttctgatgga ggagatttta ataacttgga catagcaata caacatgcaa aagatatggt
     8341 tgaccaaggt gcagatataa tagacttagg aggagagtct acacgcccag gtcatagcta
     8401 tgtagactca gacgaagagc taagaagagt aatccctgta ataaaaaaac ttaaacagga
     8461 attggagata ccaatatcaa tagacactta taaggcagat gttgcagaag aagcattaaa
     8521 attaggtgtc actatggtaa atgatgtttg gggtcttaga aaagataaaa atatggcttc
     8581 tgtaatagga aaatatgatg cagaagtgtg tataatgcat aatcaagatg ggacaaacta
     8641 tgacaaagat ataatggagt caataaaaga cttttttaag gtaagtatag aaatggcaat
     8701 gagttgtggt gtaaaaaaag aaaaaatagt acttgaccca ggcgtaggat ttggcaagga
     8761 ttttgaacaa aatattgaag tattgagaag attaaatgaa ttaaaagatt tagggtatcc
     8821 tattttgctt gggacatcca gaaaatcagt aataggaaaa gtacttccag ttgagcctaa
     8881 aaaaagatta gaaggtacaa tagctactac tgttcttggt ataagagatg gcgtagatat
     8941 agttagagta catgatgttt atgaaaattt aatggcagct agaatgactg atgcaatata
     9001 tagaaagtag gtataatata atggataaaa tattacttag taatctagga ttctatggat
     9061 atcatggtgt tcttaaagaa gaaaattttc taggtcagaa attctttgtc gacatggaac
     9121 tttatattga ttcaagagaa gcaggtctta gtgacgatat aaataaatct gtaagctatg
     9181 ccgaggttta caatgttgtt aaagatatta ctgaaaataa acaatttaat cttttggagg
     9241 ctttagcgga aaatattgct gaagaagtct tgaataaatt tatacttata aatggagtta
     9301 tggtcagagt tagaaagcct gaggctcctg taaatggtat ttatgactat tttggtgttg
     9361 agataaggag aacaagagat gaataaggct tatttgggaa taggtactaa catgggtgat
     9421 agatttgata atttatcaag agcatgtgaa cttttgaaaa atagtgattc tatatataaa
     9481 gtaaaggaat caagtttata tgaaacaaaa ccatggggat atacagaaca agcagatttt
     9541 ctaaatatgt gtgtagaaat agaaacagaa tttgaacctt atgagttgtt agaatattgt
     9601 caagaaatag agagagaact acacagagag agaatagttc attggggacc aagaactata
     9661 gatgtagaca tactattttt taatgatgta gcttccactg atgaaagact aacaatacct
     9721 catccgagga ttcaagatag agctttcgtt ttgataccac ttatggattt aaatgaagaa
     9781 cttataataa atgaaaaaac agtaaaagaa catttaaacc tcctatctgc tgaagaaaga
     9841 gaagaagtaa aggagcttgt aggttatgaa agaaaaccta tttaataata attcaaagat
     9901 agagataaat acaaataaca aaaagataac tgtaaaaaaa gataaactta taatagctgg
     9961 accatgtgca atagagagtt atgagcaact tttggagact gctaaatttg taaaaagtca
    10021 gggagcaaat attttaagag gaggagcgta caagccaaga acatcgccaa attcatttca
    10081 aggattaaaa aaagaaggtc ttaaaatatt aaaagcagtt aaagacgaag ttggtatggc
    10141 tgttattaca gaacttatgg atgtaagaga tatcgacgaa ctgtatagta taagtgatat
    10201 aattcaaatt ggttcaagaa atatgcaaaa ctttactctt ttaagtgaag tgggaaaaca
    10261 aaataaacct gttatgttaa aaagaggaat agcatccaca attactgaat ggataggtgc
    10321 ttctgaatat atagcaatag aaggaaatag taacattata atgtgtgaga gaggtataag
    10381 aacgtacaat gattatacta gaaatacatt agatttagct gcggtaccca ttattcaaaa
    10441 ggaaacaggt cttcctgttg tggtagaccc aagtcatgcc actggagtta gatatttggt
    10501 aaaacctatg tcactggcat cttttgcatg tggagcagat ggaataatgg tagaagtaca
    10561 tcctgaccca gaaaatgctt tgtcagatgg tgctcaatct ctttgtttta atgaatttga
    10621 agatttaatg aaatcaatta ataattatta aaagaggcaa gagcctcttt tttattcgta
    10681 atcattgcta aggtaaattt ataagttata atttgatagt tagattattt aatataaata
    10741 aagatttaat attgatagaa ttaatataaa aaataaaaat tttttgatta ataagaagga
    10801 tatcaataaa aaaagtcgaa ttgtgtatat tgagtgattg acaaatttag aatatagtgt
    10861 agaatagaaa tgttaacaca aaaaattaca ccagaaggtg ataatatgaa tgatataaaa
    10921 gacattattg aagaaataaa agctagatgc gatatagcaa gtataatatc tgaatatatg
    10981 aatataaaac aatctggagc taattacaaa gggttatgtc catttcatgg tgaaaagaca
    11041 ccttcttttt atataaatac atcaaaacag atttataagt gttttggttg tggtgaaggt
    11101 ggagacgtaa taaattttgt catgaaaatg gaaaatttgg attttatgga tgctgtaaaa
    11161 attttagcaa ataaatgcgg tatagaaatt aacactaaca tgaatgaaga aactaggatt
    11221 aaaatagaaa aatctaagaa atttcaagat atccatacgg aagcagctag attttatctt
    11281 tcaaacttat tgggaagcaa aaatcttggg tatgaatatt taagaataag aggactagat
    11341 gataaaatta ttaagaaatt tggtttagga ttttctttgg attcatggaa ttcacttatg
    11401 aatactctaa taagtaaagg atacaaaaaa caagacttat tggattgcgg tctaattgca
    11461 aaaaatagag atggaactaa ttgctatgat aaatttagaa atagagttat gtttcctata
    11521 tttgactata gaggaaacat aataggattt gggggaagag tattagatga ttctttgcca
    11581 aaatacttaa attctccaga cacattaatt tttaataaaa aacagaactt atacggatta
    11641 aattttgcta gaaaaaattt agaaagtaaa actatagtct tagttgaagg atatatggat
    11701 ttaatttcgc tttatcagta tggtataaaa aatgtagtag caacccttgg aacagccttg
    11761 actgaacaac aaggcatttt aataaagaga tatgcggaca ctgctattat atcttatgat
    11821 tctgatgaag caggaataaa agcgacttta agagctatag atatacttac taaattaggt
    11881 atcaatgtaa aagtgttaga cttaaaagat gctaaagacc cagatgaatt tgtaagaaaa
    11941 tacggactta gtgattataa aaaagctatg gatgtttcta ctcactacat aaaatataaa
    12001 atagatcatc ttaaaaaaga atttaacatt caaaaagatg aagaacgagt gaagttcgca
    12061 aaagaagctt ctaaaattat aaaacaattg acaagtcctg ttgaaattga tttttataca
    12121 aagtatttaa gcaatcaaat agatattaat gtcgagtcta taaagagaga agtttatggc
    12181 aaaaattaca ataagccata taataacaaa aatcaaaaga aaatagaaga aaaagtaatt
    12241 gaaaaagtcg aagtcagaca agacggaaaa caattagttg aagaaactct aataaaaata
    12301 atgttagaag acaaaaaaat tagagaaata gcgttactta aagtagaaga aagtgatttt
    12361 ttattgaaag aaagtaaaga aattttaaat tacatgatta aaaatcaaga attggacaaa
    12421 ataactattg acaaattgaa aagtttaaac atatccgaag aatacttaaa agaattaaat
    12481 tcaatttctt tgaatagtat aaacttagag aacacaaaag aaatagaagg aataataaca
    12541 aatatcagaa aaaactcatt agaagagcaa attaatagct tgttgagaga acaacaagaa
    12601 ttagaaaata ataatgatat gaaagaggta gatggtagag ttatggaaat tgctttaaaa
    12661 atagttgaga taaataaaat tttaaagagc ttgtagagaa aggaggtttg agtgtggaaa
    12721 ataaatcgaa taaaaaagag ttaaaaaagg ttactgctaa gacattaata gaaaaaggaa
    12781 aaaagcaagg ttctctgaca cttgcagaga taatggaagc tttttcggag actgaacttg
    12841 ataaggatca agtagaaaat ctttatgaga ctttaggaaa tttgggaata gaaataacag
    12901 aaacaaaaaa ttataaagct gatatagatt tttcggttgc tgatgacgat ttaagtatag
    12961 gccacttaga tgaagatgca gaggcaattt cacatgatga ctcttctgca atagaaatag
    13021 aaactgtgga tttatcttta ccaaaaggga taagtataga tgaccctgta agaatgtact
    13081 taaaagaaat aggaaaaatt cctctactta aaccacatga agaagtagaa tttgctagaa
    13141 ggatgcacga aggtgatgag atagcaaaac aaaggttagt tgaagcaaac ttaagactag
    13201 ttgtaagtat agcaaaaaga tatgtaggaa gaggtatgct tttcttggat ttaatacaag
    13261 agggaaattt aggtcttata aaagcagtcg aaaaatttga ctatacaaaa ggatataagt
    13321 ttagtactta tgcaacatgg tggataagac aagctataac acgtgctatt gcagaccaag
    13381 ctagaactat aagaatacca gttcatatgg tagagactat aaataagcta ataagagtat
    13441 caagacaact acttcaagaa cttggaagag atccaaaacc agaagaaatt gcaaaagaaa
    13501 tggaaatgac agaagataaa gtaagagaaa taatgaaaat agctcaagac cctgtgtctt
    13561 tagaaacacc aataggagaa gaagaagata gccatttagg tgattttatt ccagatgatg
    13621 atgctccagc cccagcagag gcagcagcat attctttatt aaaagaacaa atagaagatg
    13681 tacttggttc gttaaatgat agagaacaaa aagtattaaa gcttagattt ggtcttgagg
    13741 atggtagagc cagaactctt gaggaagttg gaaaagagtt tgatgtaact agagagagaa
    13801 taagacagat agaagcaaaa gcactaagaa aactaagaca tccaagtaga tcaaaaaaac
    13861 ttagagatta tttagactaa aaagatacat cattgatgtt tttgtgataa atgccatgag
    13921 ataagtgata tctcatggct attttgtagt aaagatttaa ttttattttt gtagttattt
    13981 cagtctaata tctataactt ggattaaatc ctataaataa agggaattga aaggagaatt
    14041 atatttgaaa ttaacagata gattattgaa aatagcatca ttagtgtcag atggtaaaaa
    14101 aatagctgat atagggacgg accacggata cataccagta tatttgttaa aagagggaaa
    14161 agtaccattt gcagtgctag cagatgttaa taaaggacct cttgacaatg cacataaaga
    14221 agtcatacag aacaatcttt tagataaagt tgatttacga ttgggttctg gtatagagat
    14281 acttgaaata ggggaagtag aagaagtgat aattgctggt atgggtggaa tcttgataag
    14341 tgagcttctc gaggctaaaa aagaagtagc acataatgta gaaaaattaa tattacagcc
    14401 aatgcaagca caagaagaac ttagatatta ccttttaaac aacggatatg aaattttaga
    14461 agaagtttta gtgagagaag attttagaat atatgagata attgttgcca aatatacagg
    14521 taaaaatact ataatagaag atgagatata ttatgaggtt ggaataaagc ttttagaaaa
    14581 caaagattct ctttttaatg attttataga aaaaaagata aagacatata gttctatagt
    14641 taataaatta gaagggaaaa atggggaagc tatagataag aagagaaagg aaagcgaagt
    14701 tgcaataaaa aaacttgaaa atttaataaa ataaatagga ggtatatatg ctattaaaga
    14761 gtctaactag aaaaattgaa aaaaagtatc ctctaaactt agcagaggat tgggataatg
    14821 taggtcttat agttggagat tttgatatgg atgtaaaaaa ggtattggta tctttagaag
    14881 ctaatgaaga tgttattgat gaagctatat ccaaaaatat agatttaatt gtgacacatc
    14941 atccttttat atttgggaaa attaataaaa ttaatagtgg agatttaaaa ggcagactta
    15001 tccaaaaact tattaaaaat gatatatcac tttattctat gcatacgaat tttgatatag
    15061 catttgatgg tctaaatgac tattttatgg aaattatgga atttggtaat tcaaaggttt
    15121 tagatataac taaaagcgaa aatttgtata aattagcagt atacgttcca cataactatt
    15181 cagatgaact tagaaaggtt ttaagtaatt caggagcagg tcatataggg aattatagtg
    15241 attgtacgtt ttcaatagaa ggtgaaggac aatttaagcc attagaaggc tctaatccgt
    15301 ttcttggaag tgtaaatgat atagaaactg taaatgaagt taaaattgaa actgtagtac
    15361 cacagaagct tttaggtggg gtaataagtt ctatgttaga tgcacatcct tatgaagaag
    15421 ttgcatatga tttgtacaag ttagaaaata aaggtgagat atttggacta ggtagaataa
    15481 gtaaacttga taaaagtatg acattagaaa gtctttcaag gaaaataaaa gagaaattaa
    15541 atatgaaaca cataagagta gttggaaacc ttagtacaga cataacgaaa gtagccgttg
    15601 taacaggagc tggttcagaa tttgtaaaaa aagctaagag acaaggtgca gaagtactaa
    15661 ttacgggtga tgtaaagtat catgaggcac aagatgcact tgatattgga atgtgtatag
    15721 tggattgtgg tcattttgat acagaagata tattcaaaaa tgttatgaag agatttttag
    15781 atgaatttag tgaaattgaa gtaataaaga gtaatgtata tttgaatcca tttagtacta
    15841 tttagaagtg tttgattaaa aaatactata taaaaaataa atttgagttt aattaggggg
    15901 ataatgatga aagtagcagt aatttttcat agtgtttgtg ggagtacata tttattagct
    15961 agagaatata aagaagtgct tgaagaaatg aatatcgagg tagatatatt tagagtaagt
    16021 gacgaagttg caaaaacact tcctcaatat tatttgataa attacaaaga atacaaagac
    16081 gaatttgaaa gtataaatgt aattaaatct ggaaaagaaa tattagatta tgatgctata
    16141 tttatgggtt caccaacata ttatggaaat gtatctggac caatgaagat gtttatggat
    16201 agtttttctg atatttgggt aggtgctcct ttgagtggta aaatatttgg ttgttttgct
    16261 acagcaggtt cacaacatgg aggaggagaa ttagctttgc aagctatgaa tatatttgct
    16321 caacatatgg gaatgactct tttgtcagtt ccatgttcag taagaggagg ttatccagcg
    16381 tatggaatac ttcatatagc aggagataat tctgatataa gaccaaatga tgatgccaag
    16441 ataggtataa gagattacct aaaaagactt aacatataag aatatcatat aaaaatacct
    16501 aaaagtagct gtatatactt ttaggtattt ttacttaatt ataaacgtga agctttacca
    16561 gaaatatggt caatttctat tttaacaaca gttgttaagt tcatatcctt ctctatgtac
    16621 ttcatgcctt cttcaaagaa tcctttagaa tattttttta ttatttctac aagagcattt
    16681 ttcttttctt cattttctac agtgcaagct tttccaaaaa ctatagcact agaataagta
    16741 gtactaaatt tatctggaat aacactttca ttagcaacta ctaaaaaaga aactttgttg
    16801 tttttcgata tattatctaa cttatgtcca tttcttgcac aatgaaagta aatagaatca
    16861 ttaaagtata cataatttac agcaactcca taaggataac cattttcaga tatagtggaa
    16921 aaagtaccaa attcaccatt ttttaatact tcaacagtat cttcttttgt catttctctt
    16981 tttttcaatc tcatttcttt aaacataata tcatcctttc taattttgat gcttaaatta
    17041 aattttagca catttataaa gattatgcta tgttttttac aaattttaag aaaaaaatat
    17101 agtttaagat aaaaggtagt gaaagagaag ctatttggag gtttatttat gatattattg
    17161 ttagagtagt acaaacaata ctaataaatt taatagctgg tttcttgtta tttaaggtag
    17221 taagcatgtc tataagaagg ggaagttatc gagggtagaa gttgttattt ataacgatat
    17281 tataaagaaa atattattat ataaaataat aaatagtaaa gaggtatcaa gttgaatata
    17341 atacttaata cctcttcaaa attaaattat aaaagtgttg tgggaattgt cctatacatg
    17401 gtaaggagat aataaagaat gtatctttaa agtctcaact aaacagcaaa atttatccaa
    17461 caaacataca ataaatgctt ctttgtatct aggtggctta acagtaagtg gccacatgtg
    17521 ttttaaaatt atgtccgttt ctttttcatt tatttgaaaa aataaagttg cattttttaa
    17581 tgcttctcta gggtgagtaa atccatgtaa tccttttcta tcacctttgt aatgccaatc
    17641 ataaagaaaa aagtcgtgta gtaaagctcc tctaataaca ctttgaatat caacacgtaa
    17701 gtgcaatttt ttacataata gataactata atatgctact gacaaactat gttcaagaca
    17761 tgaaacattg ccatgatgag gaaatgttct cataagttga actttttcag aagaaagtat
    17821 ctcagaagca cattcattaa aatatttgat ttcagctttt gacaacatta tgaaaatctc
    17881 cattcttagt aatatataaa tactatactt caacgataat atgaaatatt tcttatccta
    17941 caaaatataa tacccaataa tacaagtaag atacctttta tggtatttta aagtaagaga
    18001 gattttatta ggctaaacaa tgaaaaaatt aaaagtaaag ctatagcaat atatagtatt
    18061 tttccaagag gattgccaaa atttatagtc catccaatac caaacctttt attaaccatt
    18121 aatgatgggt catttggatt attatacatg atgcctgcta tccaatactt ctcatcttct
    18181 tctggagaat aagatgagtt gaattttaaa tttggggaat taatatatgt tgctattaaa
    18241 taaattataa ctaaaagcgt tgtaatattc atagcagttg atagttttgt ttttattgat
    18301 aaaagagtag taaaaaactg tgttgtcatt ataatcatca atattaaaaa caaataacca
    18361 attttattta aatatttaat gttttcaact ctactttctt caattctatt tgtatctatc
    18421 ttaatccttg actttatgga tccaatggaa gttatatata ataaaaccat taaacaaaag
    18481 tcagtaccga ttaatttaaa tacattaaga aatgatttat ctataaaaac atcaggagac
    18541 ccatttatgt tccaatgagt ggcaattgag tctggcaatt tattataatt aaatgctata
    18601 aaaatactca tccccaaagt aaatagtatc ggtattaaat agattatttt aaattttttt
    18661 attattttat ttttcttatt tataaaatcc atatctacta ttaattttga atctacatct
    18721 atatgaccta atttagaata aatttctgat ttaagcttct tagctttttt atgtgtatga
    18781 atatatagta tactctcata taataaaaaa cctataatac caataaaata tgaaagttcc
    18841 aatttattaa atgtaaaaac cagaataaaa actaatataa agtctataat aaaacctaaa
    18901 gataatagtt ttttaaattc tttatctaat tttataaagt aattataatt tttatgctct
    18961 tggtctatag aaactccata gatgtgtttt ttacctacaa aatagggcat aaaataaaat
    19021 gtagcataca tcaaaattag aactggaaat attgtaaata gacattcaat cttactcata
    19081 atatttcacc tcatacttgt caaataaact tgaacttagt gacaaaaatt cttctttact
    19141 aatacctgat aaataagatt ttgccaccaa taattccaag tcaagtttat ttttttgacg
    19201 agttgtatta tctattttca ttggtagttg agctacaata gcaccttttc ttctatcaat
    19261 atttaagaat ccctcttctt ttaactcatt gtaggatttg ttcactgtat gtaaatttac
    19321 accaatattt tctgccatac ttcttacaga tggaagtgat tcatttattt tcaattctcc
    19381 tgaagctata gccttagtta tttcttcttt aatttgtaca taaatagatt tatcactatt
    19441 aaaatctaaa tttaaaacca taaaaaacct ccattatgct ttataaatga aaacgattgt
    19501 tatatacata gtataacata aaaaataata acaaacacaa aatataagtt tatattttgt
    19561 aaaaattgta atttaaaaaa taacataatt tttattaaaa taatacagat actatttata
    19621 gatagaaata aaaattagaa aggagaaaaa atatgttaac atatttaatg aaaagacaaa
    19681 tgcataaaaa agatgatgga atgagtaaac caatgatgat tacaggagct gtaattacag
    19741 gtgtaggagc atatgctgta tacaaaatgg taaaaagcca taaatctaat tctcaaatgg
    19801 tcaatacaaa ttattatgga aatagcgatt atgatgatta taaatatgac gaatttgatt
    19861 atgactgtga ttgtagagac acaaaacaag attatgaata tgaagaatta aaaaaaaaag
    19921 taaaagaatt taattcaaga agaataaatt gtgaaaattg tccacatgtt tctcaagaag
    19981 aaatgatgca atatgtaaat agtgttaaag atgtaaagga agatattgat ttacatgaag
    20041 atgctaaaaa agataatatt tacaaagaag atgaatacaa aaaatatgaa gaagaataag
    20101 taatattttg tataactgaa caaatagtaa tgtcaaatta tttaatatct agttttaata
    20161 tgtaagttgt aacttaactt gaaagaatca gtatcattct ttagatagtt aagttacaac
    20221 ttacattttg tttttataaa ttttaatagt tctttgtgac tattttgtaa ttttagaaat
    20281 attttagtat ccagggtaat tgtgtataat aaaacttgta tagatagata gtatgaaatt
    20341 aagcagatag tttaaaatta aaatgaaaag aaaggaaata gttatgttaa agttgttgat
    20401 agttgaagat gatagtacta tatcttttgg aataaagtat gctttagaac aagaaggatt
    20461 tagtatagat atatcaaagg atttatctag tggaaaagaa atgatttctt ctaatgaata
    20521 tagcatgata ctgttggatg ttacattgcc agatggaact ggttatgagc tttgtaaata
    20581 tataagagga ttctctcagg ttcctataat attcttgact gcttgtgatg aagaagtaaa
    20641 tattgtaatg ggtctcgata taggtggaga tgattatata acaaaacctt ttagaattag
    20701 agaattaata tccagaataa aagctgtttt aagaagaaaa ggtaatactt cagaagaaaa
    20761 taagaaaata cttaaatttg gagatttaag tatatataca ttagaagcta gagtatataa
    20821 aaatgataaa gaaatatttc tgacatcagt tgaatataaa cttcttttaa ttcttataca
    20881 gaataaaaat actgttttaa gtagaactca aatattggaa aaactttggg atgtaactta
    20941 tgattttgta aacgacaata ctctaactgt ttatataaaa aggcttagag aaaaaatagg
    21001 tgatgattta tgtgaaccaa tctacataga aactgtaagg gggattggtt ataaatggat
    21061 aggaagtgaa aatagtgttt ctatataatc cagaagtgaa gaagttttta agtaagtatg
    21121 ttactttgat gtttattgtt atcataatat ctattggatt tagcataatc aacgtgtctt
    21181 taactaaaga gatggttgta agaaataatc aagctataat agggacttta tcgagtaagt
    21241 atccaaactt agaaagtcag atagtagaca taataactca agggaagtct atgaagaaca
    21301 tagattatgg tcagaaaata ttaagtaagt ataattatga taaatctatt aagataaact
    21361 ctgagcctat tatttctaaa ttagttttag agactatcaa aataaatata atattagtat
    21421 gtataatatt tattttgata tttgtgttgg ttgttagata ttttacgtca atttacaatg
    21481 atttatcaga tatgactaag tatgtttact atagttcaga gggtaagcca tttgatatga
    21541 aaaacaagaa tcaagaaggg cagataggtc ttctaaaaac tgaactctta aagatgacga
    21601 caattcttaa tgaaaaggtt gaacttttaa aaagagaaaa gatattttta aacaatacaa
    21661 tttccgatat atcacatcaa ttaaaaactc ctatgacttc tttaataatg ttgaatgatt
    21721 tattatataa tgatgtacca tacgaagtaa aaatcgattt tttaaataag ataaaaaatc
    21781 aattaaatag aatggattgg ttgattaaaa gcatgcttaa attatcaaag gtagaagcaa
    21841 aagtgataaa ttttaaaaaa gataaggtaa aatttagtga actcatacat agagcaatgc
    21901 tatctatgaa aatacctatg gagattaaaa atcaaaaact gactatagag ggaaatgata
    21961 atgtatctta tattggtgat attgattggt ctgtggaagc actagtgaat ataatcaaaa
    22021 attgtgtaga acatactcct gaatttggaa atataattat aacctataaa gaaaatccat
    22081 tattttctga actaataata aaagatgagg gtgaaggtat agataaaaaa gatataccac
    22141 atgtatttaa acgattctat agaggaagaa gtagttcaaa agaagatagt gtaggaatag
    22201 gtcttgcaat gtcaaaatct ataatagaaa gccaaaatgg cgatatatat gtaaacagcg
    22261 aaaaaggtaa gggtacagaa tttcatataa tatttcataa aatgtatgat agtgactaat
    22321 ctgtaatatt taattcactt tatagtaata gccaaaacct aatatatgaa gtatagaata
    22381 taaatattgg aggtagttat ggaaatttta agagtagaaa atctaactaa aagttatggt
    22441 aaaaatgaaa caaaagttga tgctataaaa aatgtaagtt tatcagttga aaaaggaacg
    22501 tttatagcaa taacaggtcc aagtggaagt ggtaaaagta ccttattaca tttattagga
    22561 ggagttgaca gaccaactag tggtaaggtt tatataaatg atgttgatat atataattta
    22621 aaaactaagg atttggcgat atttagaaga agaaatatag gtcttatata tcaattttac
    22681 aacttaattc cagtcttaaa tgtgaaggaa aacatattgc ttccagcaga gcttgacaat
    22741 agagatattg atagagaata ttttgatgat ttaatgaaaa cattaggtct aattgataga
    22801 gaaaagcacc ttccaaacca attatcagga ggtcaacaac agagaacctc tataggacgt
    22861 gcattaatta atagaccttc tataatatta gcagatgagc caacaggtaa tttagatagt
    22921 aaaaattcaa aggaaatatt agaattgtta aaattgtcag taaaaaaata caatcagaca
    22981 ctaataatga taactcatga caaaaatatg gcacttcaag ctgatagaat tataagcata
    23041 gaagatggaa tcataaaaag tgatgaggtg atgtagatga atttatatac atcactaacc
    23101 attagatact taaaacaaaa taaaagaaga accattgtaa ccataatcgg tataatactt
    23161 tcaacagctt taatttgtgg tataggaaat atatttgaaa gttttatgga ttatcaaatg
    23221 agagaaacta taaagaatga tggaagtttc catgttacat tttatgatgt aaatagaaaa
    23281 aatgtggaat atgtaacgaa atcagctgaa atagaaaaac atgcatttac caaacaactt
    23341 ggatattcta aattagaaaa tagtgaaaat gctatattaa gtataaagca atatgatcaa
    23401 aatgcaataa acggatataa agtttctata aaagaaggta gatttccagc taaggaagga
    23461 gaaattgttt taagtgaaag tattattaat cttatagatg ataagttaaa aataggagat
    23521 aagataactt taaaagtagg ggatatgttt gactcaagca agaaaaagat tgatagtatg
    23581 acatttcatg aaggtgatta tatagcaaat gaaaaagata gaacttttaa agttgttggt
    23641 ataattaaaa aacctgggtt tgaacgttat aatggaattg caacagctat agcctacttt
    23701 aatcctatga ataattacaa tgatacaatt aatgtatcag tagcagtaag aaatcctaaa
    23761 gatgtgtata agataagtaa taaaataagt aaaaatatat attcaaataa agataaagaa
    23821 gcagtttctg atatgataaa atacaatgaa catttattga ggttacaagg tgctagtaag
    23881 tattcaaata taaatagctc tataaagtct ataattatag cagtaactat tttagtaatc
    23941 atatgtacaa ttgctacagt gtacaattcc tttagtattt ctataaatga acgaaaaaaa
    24001 cagtttggga tattaaattc tataggtgct acatctagtc aaattaaaag attagttttt
    24061 atagaaggta ttataattag tttaattggt ataccaatag gactaatatc tggaacagta
    24121 gctatagacc tattgtttaa aattataaat aagtatttta ctgaatcggt tgttactaag
    24181 atgagtttgc agatagtgta taatccaata ataattattg ttagtataat aatagttttg
    24241 cttacaatct ttatatcaat tctactacct gcaatatcgg cttctaatat ttctcccctt
    24301 aatgtgatta aaaatactgg agagtataaa gtgggcaaaa tcaaaagctc aagacttatt
    24361 aaaatgattt ttaaaactga aggagtactt gcatataaaa atattaggag aaatagaaaa
    24421 aaatttataa taactctttt ttctttaatg gtaagtataa taatatttat atcttttagt
    24481 ggctttacat tacttttact aagaggtgaa gaaattagaa attctcaaag aaaatatgat
    24541 ttgtatttaa ctgcaaagaa cacagctagg tctgtagagg atactataag tgaactagaa
    24601 gatattgatg ggataaaaaa ctttgaattg gcaacaggac aatatgtgtc tataagagta
    24661 agtgaaaata aaataaataa atccaaggaa gatttaatca ggaaatatta tcaaaaatat
    24721 aagattggag aaagttatga atatgatttc agtaataatg aattgatttt tccgggagat
    24781 tttgcagtaa aaaacaaaat aaataactta gtacagggct cttttaataa agagagagca
    24841 attgaagaaa atggtgttat attagttaga aaaagtgcaa ttgaagaaaa tgggaagaaa
    24901 ggtgtagtag aacttaccaa ctataaagta ggagatacag taaattgtga atatcttgat
    24961 gaaaatgcct taagtaaaaa agtaaaggtc aagattcttg ctataactga tgaagaacgt
    25021 ctaggttttg gttatcaaaa tatggggtta caatttataa cttatgatga agttgctaaa
    25081 aatttaaatt taaaattaag taaaagcctt atatttattg attcaggagg aagcattaag
    25141 actaagaagg aaatagaagc tttggcaaat aaaaatgatt ttaactttta tgatgaaatt
    25201 tcaagcagtg aaagtgaaaa acaagattta aaggttatta aaatatttgt atatggtttt
    25261 atagtagtga tatctttagt gagtgttaca aatattctaa atacagtaag tacaagtata
    25321 aatctaagaa aaagagaact tgcagtaata caatctatag gggttactcc taaaggattt
    25381 aggaaaatga tatatttaga aagttttata tatggtatac tgtcattgtt gtttggaata
    25441 ccaattagta ttggtataat tctaattatg aataaactta tttcaggtgt aattgaattt
    25501 tctccgatta taccgtggac agccatagtt atctgtatag taagtgtttt tataataact
    25561 tttatagcag ggtatatacc aatgagtaaa ttaaataaag aaaatataat agataatata
    25621 agaagagaaa gtatatagat ataaaaataa ttcttaacta tataaaatat tattaaaatc
    25681 atttaagtgg tataaaatat tattatatca cttaaatgat tttttattta tataagaaat
    25741 ctaataataa attactagag gtatttaact atttagaaag aattggtatt atataaagta
    25801 agagaatata taataagaaa gaatatttga ttttatttga aaacaaaaga aaaagaaggt
    25861 gattactatt tctcatataa ctgcgaagaa tatgtataaa agtttagagg aaagaataaa
    25921 caagtttcca caaggtgctc caccatctga tacattatac aaaatactaa atgtactata
    25981 tacagaacaa gaggcgaagt tagttgctca gcttccaata aagcctttca gagtaaaaac
    26041 agcagctaaa atttggagtg taagtgaaag tgaagcatat agagtattgg ataaacttgc
    26101 tagtaaggct ctgatactag atatagaaga taataaaggg aagaaatata taatgcctcc
    26161 tccaatggct ggtttttttg agtttgccat gatgagaacg agacacgata tagaccaaaa
    26221 acttttggca gaactttact atcaatatat gaatgtagaa gaagatttta tcaaagattt
    26281 attttattca acagaaacga agcttggaag agtatacgtt caagaggaag ttttaactaa
    26341 tgataatgag gttagtattt tagattatga aagggctact catataattg atgaatcaac
    26401 tcatataggt ataagtatgt gttactgtag acatagaatg caacatgttg gaaaagcttg
    26461 tgatgctccc atggatatat gtatgacgtt tgacaatgta gcaaattctt taataaataa
    26521 taaatttgca agacgtgtgg ataaaataga atgtaaagaa ctacttcatc aagcatatga
    26581 acataactta gttcaatgtg gagaaaatgt taggaaaggg gttacgttta tatgtaattg
    26641 ctgtggatgt tgctgtgaag caatggtagc agctaaaaga tttggaaatc ttcatccagt
    26701 acagacaaca agttttatac caaatataaa ccatgaaaat tgtgtaaagt gtggtaaatg
    26761 tataactgca tgtccaatag atgcgataag caaggttaag gaagatggta aagaatatat
    26821 aaaaattgat gaggatagat gtttaggttg tggagtttgt gttagaaatt gccataaaaa
    26881 tagcattatg ttattaaaga gagatgaaaa aataataact ccagcaaatt cagtacatag
    26941 agcagttctc atggcaattg aaaaagggca gttacaaaat ttaatttttg ataataatgc
    27001 tctggctagt catagagcta tgggagctat actgtcagct atattaaagc ttgaaccagc
    27061 taaaaagatt ttagccagta aacaattaaa gtcagtatat ttagataaat tgttaagtat
    27121 gaatgataaa tagaaactaa cttaaaaaag gcaaagtttt agacttatct atatcttaat
    27181 aaacttatca tttaatattt aaatttaatt tttacacata taaaacttat aacttaaata
    27241 aaaaaaggct atattcaatt aatattactt gaatacagcc ttttttagtt atgttaagct
    27301 atttctttta tttgcttaaa tgagttttca tttccatttc caccaatttg tgttacagat
    27361 ttaaatctca tagttttaaa tagttctttt tgattataat ctagtgagtt tcctactagc
    27421 attacaggag atttagtttt tccagccaaa actccaacag ataatccgtc tattaaatca
    27481 tcttgtttat tcattccatt tttagcaaca tataaattgt ctatcttaga atccttgtaa
    27541 aattctttta ttacttttgc attagtttca tttctagtgt tacctgatat tctctgtgga
    27601 ttttgaagtt tactttctat atttttagaa actgtatatt ctccaccgat tacataagat
    27661 ttatcaatct ttttgttttt aaataagtta tttatttcag ttatattact attttcatta
    27721 gtaagtatta taggcatatc attttgagct gatacagctc caatgcttac tgcatcggct
    27781 aatccttttt cgccattaac tactgaaatc tttgatatat tgctaatatt gttcaattct
    27841 ttagcaatgt tcatagaagt ttgatatcta tcacttcctg aaattctaga tacagagata
    27901 ttgtttgatt ttatagtatc taaagatttt tcatttatag aagcctctcc accaactaca
    27961 tatacatttt taacttttaa tctctttaat tcagccaaag ttttttcatt tatttgagag
    28021 cttccagtca ataatatagg agcgtttttc ttgtatgcaa atggtgttgc ggccaaagca
    28081 tctgcaattg ctgaatcgtt tattaaaact gcattttcag cattttccca acctgcttga
    28141 cttatcttaa tagaagtttc atatctatca gttcctgtaa gtgcacttgc atttacttca
    28201 tcttgatatg taacctcaaa tctagcattt ggacttccta agaaaacaat tacgcctcct
    28261 tttggcaatc taactctatt gttctttgta actaaagaat agtttacagg aacaccttta
    28321 tcatcataaa cagcaaaagc tgaattttga ggaagattaa cttctatttt tttatttgcg
    28381 atatcgtcac caatcttata ccattttgca taaccattgg attctagttc acaagtaaaa
    28441 gatttttctg ctggcaaatt agttatagaa tcttcactaa catacgttag tgttccaaaa
    28501 gataaatact ctgtaccatt ttctttatga agtttaatgt cggataaatc acgacctatt
    28561 acaccaggta tttctataaa agcatttgag ttattctcat ccattatttt agtgttaact
    28621 atataacctg gagtttcatc agaaagacta aaacttgctt ttacagagcc aaacatatag
    28681 ctttgagaag tatatttttc atccactaga tagtaacctt tgccacttct ttttttccac
    28741 acttctttga cactatttga tatattgttg tcatctactt tttgagctac atagtataaa
    28801 cttgctgtct gacctaatcc agggacatca tcataagagc tcatatttaa atatgtgata
    28861 ttattttttt cttttacaaa ttttaaacaa ctgttaccct tttcagatac aaatctatct
    28921 tgacctatgt atacgtattt atcttcaggt ccattttcta tataaggaga agatactgtt
    28981 aaagtaccat tatcattaac atctactttt atcatatttg aagaagcata aagaccacta
    29041 ttttctttta gagagctagg cattttaact tgttgtggct tactaaatgt tttatcaggt
    29101 tttatttctt taattttacc tttttctttt aatgcagata gtaagatttc ttgtccaatt
    29161 atttcgttaa gttgactact tccaccagaa gaaagaactg caactgccat attttcatct
    29221 ggaagaacta taagattact gtggaataat aaagagtctc caccttttgt aagagctttt
    29281 aaattatact gattaaatgg ataagtgttg acacaatccc accctaaacc atatccaagt
    29341 atagaatctt ctccttcagg ccatagacca tttaaatatt ctttgttttc catagctttt
    29401 actgaagcag gagaaagaat accatttgaa tttttcataa atgtttgtgc aaatgtacat
    29461 agattttctg cacttgaata taaaccacct gcacctatag cattaaggtt atcttgtgga
    29521 actgcatctt cccaataagg tacatatgct tttgctagtt ttgaactatc aaaactattt
    29581 tctgtagttt ttgtattttg taagttaagt ggattattta tgtatttatc aagaaaatta
    29641 gtaaatgaca taccagatac tctttctact aaaatttcag ctaaagtaaa tccatcatta
    29701 caataaacgg aaaaagctcc tggtttagct tttaatcttt gtttttgtaa ttcttttaag
    29761 aaattatcat gaccataaga atcattgtct gctaataaaa tagtgttttt aaaactactt
    29821 cccatcaacc ctgatgaatg atttaataac attcttggag tgatttcctt gtatctatca
    29881 tcagccattt taaattcagg tatatacttt acaacaggag tatctaaatt taattttcca
    29941 tcatcaacca acttcataac agctgttgta gtaaacattt tactgataga tgcgatacta
    30001 tacatattat ccttatttaa gtttttgtta tcctgtttgc tgtaaactcc accattacca
    30061 gaaatctcaa ttttgccatt atcaatcaaa gcatattgag cacttacagc accatacttt
    30121 gatagcaaag tttggatttt ctctttagca agagctttag tttttgtgta agattccgcg
    30181 tttttactat aatcatgtaa ttctatatca gaactttgat atttttcgga attatctttt
    30241 gtattatcag caaaagtaat atttggagca gcagtaaata acatactcaa tactaatgta
    30301 ctagataata atttagagat cctttgctta aacatattac attacctctt tcccttttta
    30361 ataatttata attagcataa cattcttaat tattttaatc aacagattat tatcaataaa
    30421 aaaagctttt ataagtatag ctttttatat aacagagaaa aatataaaaa taatatttgt
    30481 tgttgtataa agaataaagg gtataggtaa tatataaata aaatggagga attttagaca
    30541 tatgtataag aatataaata agaagcaact taaagaaatg atgaaaaatg aaaaggatgt
    30601 tttattatta gatgtaagaa ctaaagatga atttaaggaa tataaaatag aaaatgctat
    30661 aaatatatct ttacaagatt taatcaataa tatagatgaa atttacgatt ataaagataa
    30721 gaaagtagta gtttattgca gaagtggtca tagaagtgtt actgcatgta atttgttagc
    30781 tgaagaaggt tttgagcatt tatataattt aaattcagga ataattgatt atatgagtta
    30841 aataaggtaa gttatacatt aaatactatg tatagctctg tttattaagt aggaatatat
    30901 ctaaatattt ataagataaa tttaggaaag aacgaatttt ttcgcttttt tctatgacag
    30961 tttttgtagt aaatattagt ttttaagttg gaaaaaggta gtagcttttt agctactacc
    31021 ttttaatatg aacttagaat attattaaaa aaatatattt aaagacagaa ttaatttttt
    31081 aatgaccagt taaattacta tcgccaaatt tatcattaaa tggttttata actctattta
    31141 taggtccttt tagaagtacg tttaaacaaa tagacagaat cataaatagt aatagaacta
    31201 gtatatcttt agttagattt ggagcatata taccaccaac tgcttccctt gtagcaccta
    31261 ttgcataggt aaatggcatt atagggttta ttctttggaa gaatgtaggt gtaacttgta
    31321 tagggaatgt accaccagaa gaaccaatct gaacgactaa tagtactatt gcaattgctt
    31381 ttcctacatt tccaaataca gaaactaaag aatacactat aaagttaaat accatactag
    31441 taaacataag cagaactatc aataaaattg gatgttttgc tgttactcct aacaataata
    31501 tatctccagt tcctattata aaaccttgga caagagctat acttaaaaaa gtaagacctc
    31561 ttccaaagta aatttcataa ggtttatagt ttccatgtac agatgtagac aatagtgctg
    31621 aaataagtaa tataccaacc catgtagaaa gaacactata aaatggtgtc attgcagaac
    31681 cataattttc aattggatat aatttctctg ttttcacctc aacaggttct tttaagaaat
    31741 tagactttgt caatatatca ctttcaagca agctgactaa ttttttcata tcttcaccat
    31801 tactaatttt gcttaaagta tcaatcaaat catccaacat tccttttgct aaaggaagtc
    31861 tttctcttat caatgatata ctttcctgtg catttcctga aagttttaga gaagtagtaa
    31921 gtatttcttc aacctttgga agttttgctt ctgccttatc taaaacagta attatatcat
    31981 ttgctacttt tatactgttt gcaaatatac tatttatagg ttttgatatt tttgagtcaa
    32041 aattatttaa aatatttaaa ttaattcttc caataccatt tgatagactt aaaacattat
    32101 tcaaagcact tatagatggt tgttgtccag atataacttt gtttttaata tcatttaatg
    32161 ttgatataga tgaatctatt ttattgctag agtcttcaag attatctatt acatcatcca
    32221 atctattatt tgatgtaagt tggtttaatt tagttaaaaa gtccaccaaa gtatcgttta
    32281 aagattgcaa attagaaagt ttttctgata aattatctat caacttaggt acatcttcac
    32341 tgccactatt tactgcatca attaaattta atgtaagaga agaagcagaa gatgaaaggt
    32401 caaccattaa atttaagtca gacttaataa gaggagaaag ttcgtctaaa ttatcatttg
    32461 tatcctctaa gaattttttt aagtcacttg ataatagttt tgtatcattc agagtctttt
    32521 taattgtagg taaatcagat ttaatatctt taactgtatc agataatttt gaagtagcat
    32581 cagaagctaa atccacagtc ttatttatat ggtcaaatct cttttgaagg tcaataaggg
    32641 aattttcaac ttttgtcaat tttggaagac tgtgttcaat tcctgcacca actccgtttg
    32701 atataccaag aacgatttca ctaactgttt ttactatagt ctgattaacc tgtagttgta
    32761 ttgtagaagc acctttatct gtaatctttg gtgcaactgc atttattttt tcattaacag
    32821 tatagataat ttcgcctttt ttaacatcat ctgtaattaa agatgtcaaa tcttttgtaa
    32881 aattttttgg aatcttaagt gatgcataat aagtaccttt tttaacacct tcaagagcag
    32941 ttttttcatc tacaaactgc caacctaaac tcttgttatc tttgagttgt tcaattaatt
    33001 ggtctcctac attaatcttt tgtcctgcta tttcatttcc tttatcttca tttgttactg
    33061 ctacaagcat attagcagtg cttccataag ggtcccaaga tgcctttata ttgaaccaag
    33121 catacagaga tggtaaaaaa gcaagaccta aaactattac aagtacggca taattgccac
    33181 gtatatcttt taggtcactt ttaaaaattt ctaatatatt ttttaaaccc tttgaaggtc
    33241 ttaaattctt gatattattc cagtttaaaa tttcttttaa actattagtt tttttcaaat
    33301 gtctcacctc ctaaaatatt ttgataaaga aaaaagttag atttataatc aattttaaat
    33361 tatattactt tttacacaaa tagtaaagtt aaaattttgt cttaatttta atacat
//
LOCUS       DAMKPG010000036        31834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMKPG010000036 DAMKPG010000000
VERSION     DAMKPG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.039"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(157..996)
                     /locus_tag="PC621_001820"
     CDS             complement(157..996)
                     /locus_tag="PC621_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF4108979.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            complement(1017..1550)
                     /locus_tag="PC621_001821"
     CDS             complement(1017..1550)
                     /locus_tag="PC621_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF4108980.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            complement(1562..2371)
                     /gene="aroE"
                     /locus_tag="PC621_001822"
     CDS             complement(1562..2371)
                     /gene="aroE"
                     /locus_tag="PC621_001822"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF4108981.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            complement(2418..3614)
                     /gene="pheA"
                     /locus_tag="PC621_001823"
     CDS             complement(2418..3614)
                     /gene="pheA"
                     /locus_tag="PC621_001823"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF4108982.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            complement(3617..4696)
                     /gene="aroC"
                     /locus_tag="PC621_001824"
     CDS             complement(3617..4696)
                     /gene="aroC"
                     /locus_tag="PC621_001824"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF4108983.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            complement(4674..5987)
                     /gene="aroA"
                     /locus_tag="PC621_001825"
     CDS             complement(4674..5987)
                     /gene="aroA"
                     /locus_tag="PC621_001825"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF4108984.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            complement(5987..7051)
                     /locus_tag="PC621_001826"
     CDS             complement(5987..7051)
                     /locus_tag="PC621_001826"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF4108985.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            complement(7073..8086)
                     /gene="aroF"
                     /locus_tag="PC621_001827"
     CDS             complement(7073..8086)
                     /gene="aroF"
                     /locus_tag="PC621_001827"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF4108986.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            complement(8216..8371)
                     /locus_tag="PC621_001828"
     CDS             complement(8216..8371)
                     /locus_tag="PC621_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4108987.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            complement(8548..9504)
                     /locus_tag="PC621_001829"
     CDS             complement(8548..9504)
                     /locus_tag="PC621_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF4108988.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            complement(9843..10541)
                     /locus_tag="PC621_001830"
     CDS             complement(9843..10541)
                     /locus_tag="PC621_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4108989.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(10575..13277)
                     /locus_tag="PC621_001831"
     CDS             complement(10575..13277)
                     /locus_tag="PC621_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF4108990.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(13455..15272)
                     /gene="ftsH"
                     /locus_tag="PC621_001832"
     CDS             complement(13455..15272)
                     /gene="ftsH"
                     /locus_tag="PC621_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF4108991.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(15303..15746)
                     /locus_tag="PC621_001833"
     CDS             complement(15303..15746)
                     /locus_tag="PC621_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4108992.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(16237..17127)
                     /gene="metA"
                     /locus_tag="PC621_001834"
     CDS             complement(16237..17127)
                     /gene="metA"
                     /locus_tag="PC621_001834"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF4108993.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(17157..18422)
                     /locus_tag="PC621_001835"
     CDS             complement(17157..18422)
                     /locus_tag="PC621_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF4108994.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(18532..18634)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18688..21345)
                     /locus_tag="PC621_001836"
     CDS             complement(18688..21345)
                     /locus_tag="PC621_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF4108995.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(21473..22216)
                     /gene="yaaA"
                     /locus_tag="PC621_001837"
     CDS             complement(21473..22216)
                     /gene="yaaA"
                     /locus_tag="PC621_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF4108996.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(22226..22690)
                     /gene="bcp"
                     /locus_tag="PC621_001838"
     CDS             complement(22226..22690)
                     /gene="bcp"
                     /locus_tag="PC621_001838"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF4108997.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(23042..24739)
                     /gene="ade"
                     /locus_tag="PC621_001839"
     CDS             complement(23042..24739)
                     /gene="ade"
                     /locus_tag="PC621_001839"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF4108998.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(25045..26199)
                     /locus_tag="PC621_001840"
     CDS             complement(25045..26199)
                     /locus_tag="PC621_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF4108999.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(26444..27283)
                     /locus_tag="PC621_001841"
     CDS             complement(26444..27283)
                     /locus_tag="PC621_001841"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF4109000.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(27296..27892)
                     /locus_tag="PC621_001842"
     CDS             complement(27296..27892)
                     /locus_tag="PC621_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF4109001.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(27943..28593)
                     /locus_tag="PC621_001843"
     CDS             complement(27943..28593)
                     /locus_tag="PC621_001843"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF4109002.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(28639..29892)
                     /locus_tag="PC621_001844"
     CDS             complement(28639..29892)
                     /locus_tag="PC621_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF4109003.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(29916..30557)
                     /locus_tag="PC621_001845"
     CDS             complement(29916..30557)
                     /locus_tag="PC621_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF4109004.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(30759..31172)
                     /locus_tag="PC621_001846"
     CDS             complement(30759..31172)
                     /locus_tag="PC621_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF4109005.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(31323..31664)
                     /locus_tag="PC621_001847"
     CDS             complement(31323..31664)
                     /locus_tag="PC621_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF4109006.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
BASE COUNT        10004 a         5666 c         3318 g        12846 t
ORIGIN      
        1 taaataaata tcattatcat ttaaaagata acaatagtta agggtacaaa tatatcaata
       61 gcatatttaa ttatattgaa tccatattta ttaatacaat taaatacact atcgcttaca
      121 tttataccca tctatatata gcttaaatat taatatttat ttttctaaag cttctcttct
      181 tattgatgat tttttaaaat actctatcaa tttattttta tcattgttaa atatagcttc
      241 ttttactaaa tttacttctg attcaaagtt ttctataact tttagtaaat tatctctatt
      301 tcctaaaaaa agttcactcc ataagtcttc attcatattt gcaattctag ttaaatctct
      361 ataactatct ccaataaatt tacctgtatc tctaccttct tcatcactat ttataagagc
      421 aactgccata acatgaggta actgagaagt aaaagctata atctcatcat gtttttgtga
      481 agttaacttt tttactcttt taaatccaat ctctaaaatt aaattttcaa ctaactctaa
      541 attttttatg ttgtttctgc cagttggagt aattatataa tttgcgccat taaaaacttg
      601 ttcacttgca aaatcaatac cctttttttc cctacctgcc attggatgtc caaatataaa
      661 atctatatca tctggaataa tttgcaacac atcattgata agtgtttctt ttattcctgt
      721 agtatcagta attatagtcc cttttttaaa aaagttttta ttattattta gaaaatctac
      781 aactaatcta ggatatatag atagtataat taaatcagct tttttaaata actctttacc
      841 tgtagtacat ccttttttta tgatttttgc tttttctgct ttttttaaag tttctaaatc
      901 tacatctact ccaaatacat tttcatatcc agcttttttt aatgctttag caaaagagcc
      961 tcctataact cctagtccta caataacaat attcattatt tttatttccc cctagattat
     1021 ttttctttaa agtcaaattt aaaattttca ctgactgcat ttgtaatatt atatacagca
     1081 ttatttaatg atttatcatt taaaatctct atatctgaaa attttttata aagatttatt
     1141 ctctcattat aaagattata caatctatct tttccgtttt ttaataatgg tcttgaatta
     1201 atatttatat cttccaaaat cttttgtata ggtctatcaa taaaaattat tatacctgtt
     1261 tcttttagta tttccatatt aacatccttt tttattacac ctccacctgt agatatcact
     1321 gttttatcac ttatagaaag ttctctacat gcttgagtct catagttcct aaagatatct
     1381 tctccttctg aaaacaactc tgctatactt ttttgagata tttgaattat ataatcatcc
     1441 atatcacaga aactacaatt atattctttg gccaaaagtt taccaattgt agtttttcca
     1501 ctacctggca tacctattaa tatcaatttt tcttttgttt tatttatcat gacaatctcc
     1561 cttacaaaaa ttctaatttt agttcattat atataacatc taaaatacta ttatctattt
     1621 tagtatcttg ccaaatttcc tgagatttta ttgcttgccc taccagcata tataatccat
     1681 cacaagtttt tttacccata ctattaccaa ttcttaaaaa ctctgtttct ccaggattgt
     1741 aaattaaatc tattaatata tcaaaatttt gtataataga ttttgacact ggtgaaattc
     1801 ctacattagg gtacatacct actggtgtag catttaatat tatatctcct tttatatgtt
     1861 taagttcttc ataatctatt aatatagctt tactatttaa taatttttta tcatcctttt
     1921 ttctagttga aacataaagc ttttctatac cagaatcaat aaaatatgta agagcagctt
     1981 tagaagcacc tccagttcct aatataactg ctacttttcc ttgaacatca atattagcca
     2041 ttttaaacat actatcaaga ccaaagtaat ctgtattgta accatataac atattttctc
     2101 tgagtaaaat tgtattaact gctcctatac gttttgcctc tggtgatata aaatcaagat
     2161 atttcataat acgctcctta tatggtacag taacatttac tccttgtatt cctaataatt
     2221 ttatagcacc gtctagctta gatatatctt cttttggaat ttcaaagttt ttataagcac
     2281 tttcaatatt caatatctcg aatattctct tgtggatttg aggtgaaaca ctgtgagaga
     2341 gtttttcacc tactagaccg aaaaaattca tacttacacc tcctaataat tataaattaa
     2401 ataaaaattt atattatcta ttcagtttta tttttatata ctcccataaa tttaaaatat
     2461 gctgtattat gttctatcaa ttctaataag tcgtacactt tagaattgta tatagaacac
     2521 tcaaaatcta tataaaagaa atatctccat gaggcatttt tcataggtct tgactctatt
     2581 tttgtcatat ttatattatt ttcagcaaag tatccaagta ctttataaag ttttcctgct
     2641 tcatgttcaa cagaaaatac tacactcatt ttgtttgatt cttcttcaat gtgtatttga
     2701 ttactgatta ctacaaatct tgtatagttg tttgcaatat catttataca aggacttatt
     2761 atttctaatt cataaatact tgcagctttt ttgcttgcaa ttgctgcttt tgttttatct
     2821 tgaagttttc ttacaagttc tgcacttgtt gctgtgttat taaatggtat caatttccat
     2881 gtactatttt gttttaaaaa ttcactacat tgtcctatac cttgtggatg agaatatatt
     2941 tcttttatat cttcaagttt tgtgtctttt atccctatta aattttggtc tatttttata
     3001 cattcttctc caactatata aaaaccgtac tttctcaaaa ggtcataagt ttcagagata
     3061 gctcctgtag atgaattttc tatggggatt attccatagt ttatttcttt atttttaagt
     3121 gctatgaaaa catcttcaaa ttctttaaat tgtttttttg atattgtttt gttgaagaat
     3181 ttatttagtg cttcttctgt aaatgaacct gattttcctg gaaatcctac taggatattt
     3241 tttttattta attcatcttt taaagactta tatttattta catttgacaa atcaaatttc
     3301 ttatttgatg aatctaaact aaactcgatt ttctgctcac catcattatc tatcaatctt
     3361 ttttgtaatc ctctactaat ttccattata gaattaaaaa actgtaccgt ttcttgtgag
     3421 tatgctttat tatctaaatt atctatggct ctacttagta ctttttcttc tctatctttt
     3481 tgaagtatag tagtgttatt tttcatttta tgtctggcaa tttcaagaac tgtattcatt
     3541 ctcttttcaa ataattgaat aatctcttta tctatattgt caatctcatt tctatatctc
     3601 gttaaatcct ccattattat cgtcctttca tcaaatcaaa aattccaatg gctgttactg
     3661 cttctattac aggtatagcc ctttgaacta tacaagggtc atgtctgcct ttaatatata
     3721 aaatatcgtc ctttttatct tttatattaa ctgtgttttg ttgtctgcta attgaaggag
     3781 ttggcttaat tgccactttg aaaattattg gcattccagt agtaatccct cctataatcc
     3841 ctccattgtt atttgttttg ctcttaactt gatttccttc ataatacatt tcatcattac
     3901 tttgagagcc atacatatct gcaagttcaa aacctaaacc aaactctaca ccttttacgg
     3961 caggaacaga aaacaataaa tgtgataaag tagattcaac agagtcaaaa aatggatttc
     4021 caagccctac attaatacct actactgcac actcaattat accaccaact gaatctcttt
     4081 gattttttgc atctataatc gtatttttca tggcttcttc ctttgataaa tcaagtaaag
     4141 gtagttccaa agtttgcaaa tttgataact gttgttttga aatatttaca taatcaaaac
     4201 tcatatcttc tatattttta atttttttta tgtgtgctcc tatctctatt cctttttgtg
     4261 ataaaatttg tttacatata gccccacaaa atactaatgg agctgttatc ctacctgaaa
     4321 aatgacctcc ccctctatag tcattaaatc cagaatatct cacattacct gtgaaatctg
     4381 catgtcctgg tctcattaaa tttttaagct ctccataatc ttttgaacga gtatcactat
     4441 ttcttatgat tgcacataat ggcgttcctg tagttcttcc attaaaatat ccacttagaa
     4501 tttctggtat atcactttca tttcttgaag tagatataga attttttcca ggtgctcttc
     4561 tcttcatttc tttatcaatc ttatcaaggt caagttcaat ccccgaagga agaccatcta
     4621 tatttatacc tatagcattt ccatgagatt ctccaaaaat tgatactttt aaattatttc
     4681 cccatattcc actcatcaat tcgacctcct aacatcttaa aatctttaaa aaaatgtgga
     4741 taagattttg atacacactc aaaatctttt aatattattg gtttatcaca tctacaagaa
     4801 gcaattgcaa gtgtcatagc aattctatgg tctttgtgac tccacacctc tactccacca
     4861 tttaacttgc ttactccttc gattatcaaa ctatcctctt tctcctctat atttgctcca
     4921 agtttactta actcaacatt tattgcatga agtctatcac attctttaat tctaagccta
     4981 ccagcattaa ttattgtagt cctccctata cttaaggatg ctactacaga taatacaggt
     5041 attatatctg ggcattgaga tgcatctatc aaagtactat ttaaaccatt tgttattcct
     5101 tttattttgt tgccttctct taatatttcc attcccatcc tagataaaat ttcaactact
     5161 tcactatccc cttgaagaga atcttcttta aggtctaata tgctaatatc ttctcctata
     5221 gcatctgctg ataaataaaa tgctccttga gaataatctc cttctacttt ataatctctc
     5281 gctttatacg tttgatttcc ttttataata aattctttat agttattgtt tataatttct
     5341 accccaaaat cttttatcgt tgaaagagtt aaatctaaat atcctttaga ttctagctca
     5401 gtagtaatta ttatctttga atcactctct agtgtaggca atataaacaa tagacctgtg
     5461 ataaactgag aacttatatt tccttttact ctaaattcat ctggtttaag cttacctgat
     5521 ataatcaaat ctaactgatt ttctttgtaa ctatatttta tattctgtct gtcaaatatc
     5581 tcataataag tatcaagtgg tctttttcca agattacctc tacctataaa cctttttaca
     5641 ccatcaaatg ctaaagatat aggtactaaa aatcttaatg tagagcctga ttcattacaa
     5701 tctattgtca atttaggttg attcaactga ttctctctat ttaaaatacc ttctttagaa
     5761 aaaattcccg aaatttctaa aatatcttct ttcttttcta taatagctcc taatgatgtc
     5821 attgctctta tagtagctat tatatcatca gaaaaatcta tattagaaat tctacttttt
     5881 ccattgctta gagatgaaca aataactgcc ctatgtgcca tactttttga tggtggtatt
     5941 tttacatctc cagataattt acttggatat atttttagac ttcccattat cttacctcct
     6001 gaaaaaattt agaagtagta ttatatataa tactctctcc aatatctttt aaaagtatta
     6061 tttttaatgt atcacctaga ttttttttat caagagatat agcatctact atagatttat
     6121 tatctcttaa ttcaatctca taaggcaaat tataattcat taaaattgat tttataagtt
     6181 ctgatgtacc tttttttgta attcctatct tttcactttc taaagttatc ttatacattc
     6241 ctatggcaac agcttctcca tgggtataac ccgtaaaatt gtagtatttc tcaatagcat
     6301 gacctaatgt atggccaaag tttagaagca ttctatctcc aaagtctttt tcatcagctt
     6361 caactacagt ttttttaata aagcaacatt tatgtataat gtattctata tgttgcatta
     6421 cttcttctct attttttaaa cttaaaagtt tattgaaaaa agttttgtct tttatacatc
     6481 catattttat aacttctgcc attccatctc tatagaattt ttctggaagt gataataata
     6541 catttgggtc gataaacaca gcttttggtt ggtaaaagct tcccactaaa tttttgcctc
     6601 tctcaaggtc aactccaact tttccaccta cagaactatc aacttgtgct aataaagatg
     6661 ttggaatttg aataaaactt atacctctta aaaaagttgc tgctgcaaat ccacctaaat
     6721 cacctattac ccctcctcct aaagtaataa tcaaatcttt tctagttaac ttaaagtcaa
     6781 gtaaactatc gtatatttta ggaagagtat taaatgattt tgtttcttca ccagatttta
     6841 atattaaaga tttaatttca aatccagatt cacttaaatt attttttact ttatccaagt
     6901 ataattttgc tatattttca tcagttacaa taaaaatctt atttccaaaa tatatttttt
     6961 ttatttcttc tcctatattc tttagtatat tttttttaat aaatatattg tagctatttt
     7021 cttttaaatt tactaatagt ttttcttcca cgtttaacca tccttttata gattatattt
     7081 ctctaccaac tgctgatgcg atagtcttta attcacttat caattcatca tattcatttg
     7141 gcttaatcga ttgttttcca tcacaaagtg catgtgctgg gtcattatga acttctatta
     7201 ttagaccatc tgctccaact gcaattgctg ccttagataa agagtcaacc atccatgatt
     7261 tacctgctgc atgacttggg tcaactataa ctggtaaatg actaagtttc ttcacagcta
     7321 aaattgcact taaatccaaa gtatttctag tatatgtttc aaaagttctt atacctcttt
     7381 cacaaagaac tacattttca tttcctccag ccattatata ctctgctgac attagccact
     7441 cttcaattgt agcagataat cctcttttta aaagtattgt tttatttgtt ttacctaatt
     7501 cttttaataa atcaaagttc tgcatgtttc tagctccaac ttgaattaca tctacatttt
     7561 cttcaaatat atctatatct tgtgttgaca ttatttctgt aactatagga agccctgttt
     7621 tttcttttgc tttttttaat aaatccaatc catcatattt caatccttgg aaagcgtatg
     7681 gtgatgttct tggtttgaat gctccacctc tcaaaaatcc tgcacctgat tttttcacat
     7741 ctttagctat agatactatt tggtcttctg tttcaacaga acaaggccca gctatcatag
     7801 caattttctt tgaaccaatt tccattccat taacatttat gattgaaggt tctggatgaa
     7861 attttctatt ggccttttta aaaggctcta ctacatgcat aactttttca actatcttat
     7921 ttgcttctat tcttttggca tctattttac tagtatctcc aactaaacct aaaacagtta
     7981 attctgttcc atctatggga ttaacttcta ctccttctct cccaatagcc tcaattaatt
     8041 ttttaacttc atttttatct gctcccattt ttaatactac tatcattttt actacctccc
     8101 aatattataa ttttatattt aaaataaaaa agagaactca ctaatgagtt ctctaaaata
     8161 atctatcaag cactaaatct tatataaata tataaaataa tcatttttat aaaatttact
     8221 tatgctcaaa atcactaaaa taaggcctca ccagaaagga agtacatata tttttgtaaa
     8281 agaaatagcc agcgctaaaa taaaagcccc agaacataaa gtaaaaaaag ctataaagtt
     8341 ttgctgttag ttcttttaat attcctttca tagtattatc ctccattttt ataatttatt
     8401 attcttcttg tttaataatt ataacaaaga aaatataatt gtcaatattt tctataaata
     8461 ttttcaattt aaaatatttt tttatttact taaatcagct tctcaattga tgtattttta
     8521 taaaatcaat taaacatcat ctgtaattta caataatttt tttattttaa atattataat
     8581 tccaataaaa attatacata gcataaaaaa taaagttgct ggatatccat aaatccagtt
     8641 taattctggc atatatctaa agttcattcc atatatacca gttataattg taagtggtgc
     8701 tgatatggct gttaacaagg ttaattttgt aattaagtta tttgttttct cacctactct
     8761 ggaagaataa atatctaata atttatctgc taactctcgt gttgacaatg cgaaattgta
     8821 taatttgtca attccaaaat ctataccttg aaaattttct aaattatatt tttttacatt
     8881 tgaatatttt aaatatctta tattttcctt aactattcta tccccaatat acaaaagagg
     8941 tctagtattc ttaactataa ttcttgttaa tcctcttaca tcacttattc tagatacatg
     9001 attcttatta atgttgtcca tcatttcatc ttctatttga agaattaact cttcaacttt
     9061 ttccaaagac tcaaaaccat tttttataac ttctctaaga atcaagtagt ttatcttaaa
     9121 tagattaata actggatttt tttcaagttg agagttcttc aaaataatat tttttacaaa
     9181 ttcaaataaa aagtgttctt cattcactac aaccaatata aaattatctg ataaatacat
     9241 atttacttct tctataacag cttctccatc tcttagttca aatgtattta ggcttaaaaa
     9301 atcgtaatta tcaaataaat caagtttggt tatttcatca aatttaagac aatcattaaa
     9361 agttatttca tctatatcca aagtttcctt aagcaatttt aaattttttg gagcacttaa
     9421 tataagataa gaattttctg ctacataaaa tttatctcta aaatcttcaa tagtctctct
     9481 tgtatttaaa ttcagtatat acatacatct actcctaaat ttcaatttac attctaaaaa
     9541 ttttttataa tccttactgt aattatttta cactatttta acaattatta tttaatattt
     9601 acattaaatt tattataagg taagtgttaa atacatttat tatttaacca aagtttttat
     9661 tacagattac actatatact tattttaaaa catatttcat attattgata ctaaaaagct
     9721 aaaaaataaa atgcatgtgc taaactgaca acaagcacat acacttcatt cttattttaa
     9781 ctaaactata taattttaat atatattaat aagcacataa tctaataatt aatatattac
     9841 aactattcat catttaatct gtatcctaca ccaacttctg taattatata tcttgggttt
     9901 gcaggttgtt tttctatttt tcgtcttaat gttgccataa agacccttaa agacttagtc
     9961 tcattcccta taacactacc ccatatttct tttattataa aattatgagt aagcactttt
    10021 ccatgatgct ttgcaagtaa tgaaagaata ttatattcaa taggtgtcaa atggacttca
    10081 acatcatcaa caataacttt tcttttatca aaatcaatca ataatttatc tacttcaaac
    10141 gtagatttaa catcatcttt ttgttgagct tctacttgtg aatgtctaaa tgcgactctt
    10201 actcttgcta ataactctac tatggagaat ggttttgtta agtaatcatc tgcccctgca
    10261 tcaaatactt ctaccttatc tctatcttgc tctctagctg aaactacaat tattggtata
    10321 tttgaaaatt gtctgatagc ttttattacc tctattccat ccatatcttc taagcctaaa
    10381 tctaaaagaa caacatctgg agaatatgag acagcaatag atataccttc ttttccagtt
    10441 atagcctcct tgacatcata atcctgtgtt aaaagtgcag tagatataaa ttttcttatt
    10501 gtcttatcat cctctactaa caatataagt tttttactca tttcttcctc caaaactaat
    10561 tttaaaattt aaaactattc attttcctta ggcaaactaa acttaaaagt tgaaccctta
    10621 tctttattgt tttctaccat aatttcacct ttatgagctt gaattataga tttacaaatt
    10681 gataatccaa gtccaacacc ctttctggaa tctttagctc cttcttctcc agtaaaaaat
    10741 ctgtcaaaga tatgcttttt aagttccttt gatataccag gaccattatc agatacttca
    10801 aaccaaacat aatcatcttt ctcgtataca tttatctcta tcttacaatc ttcctttgaa
    10861 tatttcaaag aattatctat taaattaatt aaaacttgtt ctattaattt tgcatccata
    10921 ggaacaaata atatctgttc tggtatatta atatctataa gtgcattttc aattcttttt
    10981 tttatttttt gtaacgcttc tgatataatt tcctcaacca gttcagaatg ttttttaact
    11041 tccaatttcc cttcatctat cttggtcata ctaagtaaat tttcaacaag tcttattaac
    11101 cactgagtat cttcatatac accactaagt aattcatcta ttatatcttg acctatagta
    11161 cccttgtttt ttataattgt acttactgca ccagatattc ctgctagtgg agttctaagg
    11221 tcatgcgaaa ctgctcttaa aagattactt cttaatcttt ccctttcaat ctcaagattg
    11281 gtattttctt tagcttctga caagatttct ctatcaagcg ctattgccat ttgagcaatt
    11341 attgtttcaa ttaagcttat atcttctgta tccaattttg aatcaataca tgatattcct
    11401 actactccta aagttaaatt cattcctaca attggtatat agtatccctt tgaaccagga
    11461 agtgtatccg tgtcatttcc tgccactgtt gaattggata aaacccaact tgcaacagct
    11521 ttttcatctt cactatccaa atcattttta tccttattat tagtattttt tttgtaaagt
    11581 tttgatttat tatgcttatc tacatttaaa taacaaatta catctctact taagctaaga
    11641 gacaaaagtt ctataccttt gtttactata tcatctttac ttgataatct taaaaatccc
    11701 ttaattattt gatatatcat ttttgtattt tcttctcttt tagcagcagt ttctgcttca
    11761 tgctgtatct tagacgtaag agtgctagtt attataccta ctattgagaa tacaaccagt
    11821 gttactatat agtttgaatc tgctatagat aatgtatata atggtgctgt aaaaaaataa
    11881 tttaaaagaa ctatattaaa tacagaactt attatacctg tactataccc tttagtccac
    11941 atagaaacta aaactacacc caacatatat attaacaata tattttctct tataaatccc
    12001 atgctttgta ctgcatatgc taaaatactt atcatcaaag taataaatat aagttttata
    12061 aaatcaactt tgctaatttt aaatttagat tttattgatg atttatcctt tttaggtttg
    12121 tatttaacat ctgaggcaaa tttatatgga attatatgta tatctatata atcaacttca
    12181 tcaattaatt tgtcaactat atctttttta aatcttttac taaatctttt attacttgaa
    12241 tgatttctac ctattacaat ttttgttaca tttctcagtt ttgcaattct aagtatttgc
    12301 tctattatat tttcaccatg taaaacaatt atttctccac ctaatttctt agctaatttt
    12361 aggttatttt gtagttgttt ttgttcatca aatggaagtc ttgctgtgtt tggagtttca
    12421 acatataaag ctatccattt tgcaaatgta ctgtctgata ttctggatgc agttctaata
    12481 acttttgctg aagatggaga tggacttata catgcaagaa gtgtatctga tgtaggtatt
    12541 acagttacat caccctttga tagtctggtc atttgaactt ctttattaac tctctctgct
    12601 gtttttctaa gtgcgatttc tctaagtgca accaggttat cttttataaa aaaattattt
    12661 tttgctctag tagcttgttc tttcttatat atcttacctt cattgaacct ttcaagaagt
    12721 acatcaggtt caacatctat aagttctaac tgtgtatttg tatccaaaaa cttatctggt
    12781 atagtctctt taactgatac gtttgtaatt acctcaacaa tgtcatttag actttctaaa
    12841 tgttgtacat ttacagtagt gtagacattt atgcctgcta acagcaattc ttctatatct
    12901 tgccatcttt ttgtatgcct taacccagca acattagaat gggcaaattc atctactaat
    12961 ataacttctg gttttcttgc taaagcagca tctaaatcaa attcttttag ttttatagat
    13021 ttatattcta tctcctttac aggaagaatt tccaagccat caagtagagc caatgtttct
    13081 ggtctagtgt gtggttcaat atatccaact actacatcta cacccacttt ttttaaattt
    13141 tgtgcagatt ccaacataga gtaggttttt cctactccag ctgcataacc aaagaatatt
    13201 tttaattgac ctctacttag tttttcttct tcatttttaa ttttttctaa tagaatatca
    13261 ggattaggtc tatccattaa aaaacctcct tacaccattc attactatta taatatcaat
    13321 tccgatataa aattgtagtt aagatgtatt tttaatgatt aagattatgt aaagatttaa
    13381 atatgaattt aaaaaattta gttgtaaaat aaaaatgcct aacaataggc atttttatct
    13441 tgtgtataat cattttagtt taagttaagt atttccataa actcatcacc tgtaatagtt
    13501 tctttttcta ataaataatt agcaagttca tgcaatttat caatattatt ttctaagatt
    13561 tcaattgcat ttttgtgaca agcctttact attttaaata cctcttcatc tattttagca
    13621 gcagtgtcgg aagaacaagc aagagaagta tctcctccta aatattgatt gcttacagtt
    13681 tcaagagcga ccatatcaaa ctcttcactc ataccaaggc gagtaaccat agctcttgca
    13741 attttagtag cttgttctat atcatttgat gctcctgatg tacaagtgtt aaagataacc
    13801 tcttcagcag cacgaccacc tgtaaaagta gttattttat caattgcttc ttctttagac
    13861 ataagtacac tctcatcttc agcaacttgc atagtatatc ccaatgctcc tgaagttcta
    13921 ggtattatag taatcttatg gacaggtgca gaatgttttt gcatagcagc aacaagtgca
    13981 tgacctattt catgatatgc tataattttc ttttccttat ctgaaattac agctccttta
    14041 cgttgatacc cagcaattac agtttcaaca gattcatcaa aatcctgttg ttctactata
    14101 tctctgccag acttaactgc taaaagtgct gcttcgttta caatatttgc aagttctgca
    14161 cctgatgctc ctgatgtaga ccttgcaatt gcattataat ctatatctga gcttacattt
    14221 acattttgag catgtacctt taatatagct tcacgtcctc caaggtctgg aagttcaact
    14281 ggtatacgtc tatcaaatct tcctggacga agtaatgctt tgtcaagact gtcaggtcta
    14341 tttgtagcag caagtattac tactccaatt ccaccatcaa aaccatccat ctcacttaac
    14401 aactgattta aagtttgttc acgttcatca tttccaccta atccacttcc attatcacgc
    14461 tttttaccaa tagtatcaat ctcatctata aacactatac atggagcttt ttcttttgct
    14521 tgtttaaata aatcacgaat cctagaagct cccattccta caaacatctc aacaaactca
    14581 gaaccagata ttgagaaaaa tggtacattt gcttcacctg ctacagcttt tgcaaggagt
    14641 gtttttcctg tacctggagg tcctactaaa agtgctcctt taggcatttg tgctcctatc
    14701 tttttatact tttctggatt gtgtaagaaa tctactattt cagttaaagc ttcttttgcc
    14761 tcatcttgac cagctacatc attaaatctt tttccacttt gagcagatac atacattttg
    14821 gcgttgcttt ttccaaattg catagcatta ccacccattc gcttttgcat agacttcatc
    14881 attaagttac caaatagcat aagaatacct ataggtatta tccatgtaag taaaatactt
    14941 aaaagaggtg aattttcctt aggaataacc ttagtaaatt ctacttttgc attttttagt
    15001 ctatctacca attctgggtc atccattcta ccagttatgt atatattctt atccttgcta
    15061 ctagatggat ttattgctat tctattattt tctatctcaa cagatttaac ctttcctgta
    15121 tccaacattg tgagaaactt tccatagtca atctcaacca tttttttatc cattaaactt
    15181 ggaaatatta aagcatttaa aaacattact actattatta taactgtata ataatagagt
    15241 gctgaccttt tttggggttt atgttcatcc ataattttat tctcctttaa attattttta
    15301 tgttatttgc aatcaatgta agttaaaagt ttatttaatt tctctaaacc ttctattata
    15361 tcttccatat cttgcaattt ttcactggtc atattttcaa aataactatc aaaaatacta
    15421 cttgaaatat gttcttgtat atccaatgat ttatttgtaa cttttacttt tacaattcgt
    15481 tggtcttcta tatctcttat gcgatttaaa aagccatttt tttcaagcct ttgacagatt
    15541 actgatacat tactatttgt catgtttaag ttcttcccta aatcgcttat ttttttctct
    15601 ccatgttcac ataactcaga taaaatcaaa gcttgcactg ctgtaatacc aagtggctga
    15661 taaaaatcac ttaataaagt tgtaaatttc atatttatat ctctagtcaa ctctagtagc
    15721 tctttcttaa aactcatctc atccatacaa acgctcccta cgaaaatact ttatttcaaa
    15781 tttaggctga agtgcatact gtaaataaat acttaatact ataatgttta atgtattaaa
    15841 tattatagta ttaagtattt attgtcaatt acaatattgt aacttcaatt ggaattttgt
    15901 tatcttattg atatttaatt ttatacagga tatttttaat aaaatttaat aaaaaatcga
    15961 tatatatatt ttacactgat ttctcccttt ttaatctctt ttaaaagtta aaatacaaat
    16021 ttattgatac tataaaggtt tagtctccca ttatcgcacc atatataaaa ttaaaaccac
    16081 ctctatctac atacacctaa agtgattttc ttaaaatata aaactactat tataaggtta
    16141 aatttaaaaa aaataaggta taaagtatca ttaagattaa ctttatacct ataaatttct
    16201 atattttttt agttaacatt tatatactct taaaatttat tgtatcaaat agttttttat
    16261 ccaatttcta aacaacaatt ctgaatgttt tttccatttt acagttggct cttctgaagg
    16321 gtcatcatta atatagtaat tttgaggtat agatatttta tctcctttat ttatatccct
    16381 cttatattct ttatcaagag tgtatttgtc atactcgcta tgacccatta cgaacacatc
    16441 tcttgaattt gtaattatat agggaccagc ttctttagaa tgactcacaa tgctcaactc
    16501 tttattctct ttcaaagctt caatatttac agtagtatgt cttgaatgtg gagcaaaaaa
    16561 ttcattttca aatccatcta ctattttact atctttatct accttatgct taaatactcc
    16621 aaaacatttt tgacttagtg gaagtttttt tacattgtag tatttgtaaa gacttgcttg
    16681 agctgcccaa catatatata atgtagattt agtttttctc ttagaataat ccataatttc
    16741 ttctagttca ttccaataat caacttcttc aaactccatt tgttcaactg gagctcctgt
    16801 tattataaag ccatcaaaat actctccttt ggcttcatca aatgttttat aaaattcttt
    16861 aacatattca ttacaacttc ttttactttc tcttgttgaa attttaataa attcaacaga
    16921 cacgttgaat cctgttttat ctatccttct tagtaaatct aattctgtat ctatctttat
    16981 aggcattaga tttagtatag ctattttagt gtctatgttt tcttcatact ttaattcctt
    17041 tttaaaatct tctttataaa ttacatctat tccttcatct aataatttat taataacagg
    17101 taatcccttt ggtagtatta aagccatatc ttttcacttc tctttctctt agaatattag
    17161 aaatttaaag cttgatttaa atcagctatt aaatcctcta cattttctat tccaacagat
    17221 aatcttatta gtgaaggcaa tactccagat gcaattaatt gttcttctgt taattgtcta
    17281 tgagtagttg aagctggatg tataacacaa gttcttacat ctgaaacatg tgtaaccaaa
    17341 gatgctatct gtaatttttc cacaaatttt ttggcatttt ctaacccacc tcttactcca
    17401 aatgataaaa caccactaca tcctcttgat aaatactttt tggcattctc ataatacttg
    17461 ttatcttcaa gtcctgggta attaatccaa tctacatttt catgtttttc taaaaatcta
    17521 gctattttaa gtgcattttc actatgtctc tccattctaa gatgtagtgt ttcaacattt
    17581 aaattagtaa gatacgcatt gaatgggctt aaacaatttc catagtctct aagcaactga
    17641 actcttgctt tagttgcata tgcggcattt ccaaattttt gagtatagct tattccatga
    17701 tatgttgggt ctggctcaac taattctgga aattttccat tatcccagtt aaagtttcca
    17761 ccatccacta taattcctcc aacacttgaa gcatgcccat ctaaatattt tgtggtagaa
    17821 tgagtaacta tatttgctcc atactttaaa gggttacaaa gcactggaga tgctaatgaa
    17881 ttatcaacaa taaatggaac attaattctc ttagctacat ttgctatttt ttcaaaatct
    17941 atgacttcaa gagtcggatt tgcaagtgtt tctgcaaata caaccttagt attttccttt
    18001 gcaagttcta ctatctcatc ttcacttgaa tctaagtcaa acgatattaa atctatacca
    18061 aattttttaa gactaggtcc tagtaaattg aatgttcctc catatacttt tgaagaacaa
    18121 agtatactat ctcctgattt acatatattt aaaactgcca acatatttgc agattgccct
    18181 gatgatacag ctacagaaga tactccaccc tctagtaaac ttattttttc ttcaaaagct
    18241 tgaatagtag gattgcttat tcttgaatat atatgtccat caacttttaa atcaaataat
    18301 tcagcaagtt ggtctatact gctatattta aaagttgtac tttgatataa aggtaatact
    18361 cttggttctc cattacctgg tttataattt ccttgcacac atattgtttc tttattatac
    18421 attcttgtat cccccttaga ttttagtaaa aaaaattaaa ctatttatca gatgagagtt
    18481 aaaataaata aagcctgagt atataactca ggctctgtat aagcttaatt atactcatct
    18541 ctcagctttc gccgcaggat ttagcaccaa tgtatataaa tatactggtt gccgggtttc
    18601 atagggccag tccctccacc tctctggata agatttattt aatttttcta tgttttatat
    18661 cttatatatt tttcctaatt ttgtcaacta tttttttctt ttttttcttt taataatttt
    18721 cccaacctct acttgaatta cagacattat ggctaaaaca atcgttataa gccactctaa
    18781 gttatttaaa actgttaagt taaaagcatt tctaaaaaca ggtacaaaca gtattactgc
    18841 tattaaaaat gcagaagtta aaaatgagat taaaagaaat ggatttttac cattatttct
    18901 attaaacatt gaatctgtat ttgaacgctg actaaatgct cgtaacatct gtgaaaatgc
    18961 taatacacaa aatgccattg tttgacctac agaatgattt tctatattaa gaccaatttt
    19021 atatgctaaa atagtcatta ttgccacaaa tataccttgg gtaacaactc tttgtataag
    19081 atttttttca aacaaagtgg ttgatttcac tggctggtgc ttcattatat ttctgcttgg
    19141 tgggtcaact ccaagagcaa gggctggaag cgtagcagtt gcaaggttta cccatagaat
    19201 atgaacagca agaagcggag tatcccaatt taataatatt gctacaaaca acgtaagtat
    19261 ctctgctata ttaccaacaa gcaaaaattg tataactttt tgtatgtttc tatatacacg
    19321 tctaccttct tttattgcat aagcaattgt tgtaaaacta tcgtccatta aaatcatgtc
    19381 agaagaatct tttgcaacat ctgtaccact tatacccatg gctataccaa tatcagcaga
    19441 ctttaaggca ggagagtcat ttacaccatc cccagtcatt gctggtactt ctccaattct
    19501 ttttagagat tgtataattc taagtttatc aaacggggac acacgagcaa atactgtagt
    19561 atttttaaca gcttcatcta gttcatcatc actaaaatta tcaagttcat caccagatat
    19621 cacagtattt tcttcactcc atattcctaa ctcatgagcg attgttgtgg cagtaacttt
    19681 atggtcacct gttatcatta tggttcttat acctgcttct ttacaagtac ttactgcatc
    19741 aattacctca gttcttggtg ggtcaatcat accaaccata cctataaatg tcaaatcata
    19801 ttctatattt tcactatctt cttttggtat agatgaaatc tcacgcttag caaaaccaag
    19861 tacacgcaat gcatctttag acatgttaag acataattcg tgtatattct ttttatccac
    19921 ttcggtaatg tttctttcac cttttgaagt taatatttta gtacataggt cgagaatttc
    19981 gtctactgct cctttagtat aagcaataat cttttcatct atattatgaa cagttgtcat
    20041 acattttcta tctgaatcaa aaggctgttc aaatacacgt ggatattctt cttcaagagc
    20101 ttcttgatct attccaaatt ttttggccat aaaaattaat gcgccctctg ttgggtcacc
    20161 taatatttca tctgttttat ctggattaat acatgcatca ttacaaagtg caccagcata
    20221 aacaatctct ttatacacta atggatgttt ttcagatgca gtatctattg atgtcacttt
    20281 tccagattca aaatctccat tgacagcaat atgtgtaaca gtcattttat ttaaggttaa
    20341 tgtaccagtt ttatcacaac atattactgt agctcctcct aatgtttcaa cagcaggtaa
    20401 ttttctaatt aaagcatttc ttttagccat acgctgtacg cctaaagcca ttacaatagt
    20461 agctgttgca ggtaatccct ctggaataat tgatatggca agggaaattg caatcataaa
    20521 cattggcaag agaggtctct gataaaatgc acctattgca aatataacaa cgcatacaat
    20581 aatacctatg acacttaatg tctttccaac agtattaagt ttacgtttta taggagtatc
    20641 aaaatcatct tgtgaatcta aaagatgtgc tatgttaccc acttctgtat ccataccagt
    20701 tgctactaca acacctacac ctctaccata tgtgactatt gaagatgtat acgccatatt
    20761 gctacggtct cctaatacac attctttagg taagattgta tctgcatctt tttctgatgg
    20821 aacagattct cctgttaaag aagcctcttg aatttttaaa cttgatgttt ctattaaacg
    20881 aaggtctgct ggaaccatag ctccatcttc taaaaaaaca atatctccta ctacaagatt
    20941 acttgctgga ataacatttt cttcaccatc acgcataaca cgagctgtag gtgagttcat
    21001 attttttaat gcttctaatg atgattctgc ttttttctct tgaacaattc caataactgc
    21061 atttactgtt acaattatta gtattacaaa agcttctgta aattcgccaa atatcaaaga
    21121 taatattgct gctcctatta gtatcataac catagcatct gtaatttgac tccaaagcat
    21181 ttgtaaagtt gttctaggtg gtttttttct aagttcattt aagccattct ctttaagccg
    21241 tttttccgcc tcggtatcgg tgagcccctc ctctgatgaa tctagacttt ctaaaatttc
    21301 atgcgttgct cttgaatgcc aaggcatctg tttgtgattt tccattttgg aagaccactc
    21361 ctttcttttt ttaatttaag aataattata ttttaaaatc aagttcaatt caatacacac
    21421 taagtatatt ttacctaaat ttaacaatgc catttaatat ttaacaaaac ttctactctt
    21481 ttacaaatat gtattcacta ttgttagata agttttcttt atacttataa ccttctaaat
    21541 taaacttttt cagttcttca atatctctta tcctattttt tacaatgtat tttgtcatca
    21601 tacctctagc tttctttgct tgtgtagatt ccactttcaa tatgccattt ttatttactt
    21661 taaaagtaca tgtaatatat gtatcactat ctttaataaa tttttctatg gacttgctgt
    21721 attcatttga tgaaagatta atgatagctg ttttttcttt gattagttct ttatatatac
    21781 gatttcccca atattcgtat aaattcttaa actcacctac tcgtaatttt gtctgcattt
    21841 ctaatctata ttcatatata ctatcatatg gttttacaac accatacaat ccacttatga
    21901 ttcgaataca gctatttgca aactcttcat ctttccttgt aaaattttct gcttctatat
    21961 ttttatattg tattccatca taagttaaga ttgcagggtt tcctaatcta tcaaatttaa
    22021 tgctttcaaa tctattttta tttaatacag ctaatttttc attaattttc attttatttt
    22081 taaaatcttc tataccaaat gtctttatat tttccactat ttcttttgtg ttatctataa
    22141 aacaaggcaa actcaaatct ctatcaaata cttctatatt cttcatattt ttagctggtg
    22201 ataacattat aatcataaat tcttattact cctgtttttc caaatattca agaatctctt
    22261 gtgcatttgt gtctggtttt gctttttcaa agactttttc aataattcca ttttcatcta
    22321 taatatatgt cgcacgtaca actcccaatg cagtttttcc atacattttt ttctctttcc
    22381 atacatcaaa agcttttata gcaactaagt cagggtcaga aagtaaaata aaaggaagtt
    22441 catgcttttc tgcaaatttt tgatgtgatt ttatactatc tttacttatc ccaataactt
    22501 gtatatcttc ctttttaaat ccatcatatg catttctaaa agcacaagct tgacgtgtac
    22561 atcctggtgt attatcttta ggataaaaat atactactac ttttttgcct ttaaaatctg
    22621 acatacttac cttatttcca tctttatctt ctagtgtaaa ttctggtgct tttgttccta
    22681 tactcaacat aaatatctct ccttttgttt ttattaataa ttttatacta tgcttatatt
    22741 attaccctat aaatctatat ataaataaaa tattttattt tttatgtata aagtttcata
    22801 aatatataat tttcatgtat ttatattcta aaatagactg aaaatagttt tattaagata
    22861 tctatgctta aaacaaggtt aaatgataga ttaattaaaa taaggatatc taaaaataaa
    22921 atttttagtt cattagatgc tcttttttgt atgaaatcaa atatattttc atcatttcaa
    22981 caataaaaag agacttatcc ctattttgag aaagcctcat ttattattac ttttatatta
    23041 tctatataaa tttaaattcc tcaacatcaa atagacctaa atctgtaagt cttatttgtg
    23101 gaacaactgg aagtgccata aaagaaagtg taataaaagg gtctacatac tctggaactc
    23161 ccatttttct tgctatctcc aacatattat cagtaatttc ttgtacttct tcagctgtaa
    23221 aagtactaat taatccagct acttgaagac ttaggtgtgc aagaactttt ccatttgata
    23281 caattacata accaccttgt atttccttta agtaatttat agctactaga atatcatcat
    23341 cattatcccc tgcaactata atattatgag aatcatgggc cacagtagta gcaattgctc
    23401 cattttttat gccaaatcct tttagtgaag caattgctac atttccagtc attctatgtc
    23461 tctcaacaac acaaagtttt gaatactctt tatttggaac aaaatatcca tctgcacatg
    23521 gtaaagattc atatacctta tttgtcaaaa tttgataagg tacaatctcc attacataat
    23581 ttttttcact tacttttaac tgtatttttt ctttattaat atacttaaac ttgactgtat
    23641 taagcaattc tttatcttta atctcataat tgtaattaga aaacatctct tcgtttacca
    23701 aattaccatc tttatataca ctatccactt ccattttgtc taaatcattc aatacaacaa
    23761 tatctgcctt ataacccgca cctattgctc caattttcct taacccatat gcttttgcag
    23821 aattataagt tgcaactttt atagccctaa caggttccat acctaaatca atcgcacatt
    23881 ttatattcca tcttatgtga ccttgttttt ctatatcatc taagtgctta tcatctgtgc
    23941 aaaacataaa ttcttcaatt ggcaagttat gctttagcat accacttact atagacttta
    24001 aattttttgc tgcgcttcct tctcttatta aaatttttaa tcctgctctc aatttttcat
    24061 atacttcatc aaaagttata cattcatggt cattttctat tcctgcacat acatatgtct
    24121 gaagtgattt accatttata ttaggtgcat gtccatctac aactttattt ttaaaaagct
    24181 ctagtttatc caagatttca ttttcagaat ttataacatc attaaaacac attacctctc
    24241 ctaaacctaa aattctagga ttatttacat aactctccat gtcttttgca ctaaatttac
    24301 caactccatt agtttcaaaa cttgttgcag gtacggatga aggcaccata atatatacat
    24361 taagtggtat atcttttgtc gattctaata aaaaatctat tgcactagct ccttttacat
    24421 tgaccaactc atgtgggtcg gctatgatag ttgtcgtacc tgattttaat aattttttcg
    24481 ataattcaat tggcattacc attgtagatt caatatgcat atgtgcatca ataaagccag
    24541 gtgatatata tttattgcta caatcaattt ctacattgcc cttgtaatcg ccaatgccaa
    24601 cgatagtgtc tcctactatt gctacatctc ctgttattaa ctcttctgta aatacattta
    24661 tgattgtccc attttttagt acaatgtcag ctttttctct tccacttgaa acatcacact
    24721 gtctttttaa attacccatt tttacaactc ctcctaagtt ttttatatca atatactggc
    24781 tgtattacgt attatagttt aatttattaa tatatattat atatgagttt tgatagaaag
    24841 tatcacttga aatttgttcc ataattcctt taattaaata gagagaacat gaggaataat
    24901 attatctccc atgttctcat taatatttaa gcaataattt aattcatatt gaatttttta
    24961 tttttatatt taatcttcac tattcttata aataaattta tgtgctttat tattttttta
    25021 atacttcttt attcgtaatt atatttaacc taaaaatgca taaactatca tacctacaag
    25081 tattgctggt atagtagtaa taatcatagc ccataaagaa gattttttat ccattgctaa
    25141 aagtgggaaa agtgcatctc catcttgaga aatagagttg gctatcactg caccaagtgg
    25201 tatagttccc ttcaaataaa atgacataag cacaatttgt atcccacaac ctggaataag
    25261 tccaagacct gcacctataa atacacttat tacacctgtt gataatacta gtttaactaa
    25321 gtattcatct ccacctaaaa ttaatattat caaatcgtaa ataagatatg caataaaaat
    25381 ccatgtaatt acaaatgata tttctccaac agaatggata agcatttctc gcaaagaaat
    25441 ctttttattt tcttcctctt tttgattaga atttttaaat acctttttaa aacaccacat
    25501 ataaattatt gaaaataata ttccaatcac tgctattact tcttccaatg aatgtaactt
    25561 ctcaattata ggaagattaa gtccagagtg agcaagtgac ataaatataa aaccgattac
    25621 tataattcct atataaaatt tatatccctt tccatgtgtt attataaatg caagtctatt
    25681 tgtatttcca tgagatttag ctagagtgtc taaaattatt tcatcatgat tatgttgttc
    25741 tttattttct ttagggttat ttttacttat atcatattta tctgattttc tacggttttt
    25801 taaccctaat ttttcttcta gtttaaagta atccactaat tgtcccgtta ttattcctgt
    25861 aattgtacta actatagtga caaaaaaata cattttaata tttgatgaaa taagtacaaa
    25921 tgctgcatct cccatactag ctattaaact tgcaattatt gctccaaagc ttaattttcc
    25981 atttatatac aatggcatta ttgccagact tccgccacaa cctggtatag ctccaattaa
    26041 agcacctatt aagggttgaa attttctgtt ttttgatatg atattagtaa aatttccact
    26101 agttttataa ttaatatatc caaataacaa aataaaaaat ccaatcatag aacctacatg
    26161 taaaaacgcc tcttcagaag cggatatcaa catttccatt aatttacctc ctagactaat
    26221 tttgcaacaa ttatattata ttctattttc tattgataat caatatcaat atttcttttg
    26281 aatttttaat attaaaaagt gcgcatctta tactcttagt ttttcatctt aagatacaca
    26341 ctttttgata ttaggttatc tatttaccac atgtaatatg aaatcaaagg tggatattat
    26401 gataaattat aacaatgtcc acctattttt attacctatc aaattataaa ttttcaagga
    26461 atgttacaac ttcttctatt atccaattag gtgtagatgc ccctgctgta atacccaaat
    26521 ttttatattt tttaagcgta tttacatcca attcatcttt agtctctatt gcaaaagttg
    26581 gtactaagtc ttcacaaacc tttactaact tttgagtatt tgaactgtgt ttacctccta
    26641 taactatcat acaatcaact tctttagcta actctttagc tgattcttgt cttgtttttg
    26701 tagctgaaca aattgtatca ttaaatacta tttcttctaa tttatctgat agtttattaa
    26761 ctatacttat atacaactct ggatttattg tagtctgtgc aacaagacaa tattttttgg
    26821 agttattaaa ttccatattt tccatttgtt ctaaggtctt tattattata gctgaatttt
    26881 cacaccatcc atttatacct ataacttctg gatgcttcat atctccaata actattattt
    26941 catagccttt attatgatat tcacttacta ctgtatgtat tttctttaca aatggacatg
    27001 tagtatccac aatttttaat tttttatctt tggcttcctt atatatattt tcaggaactc
    27061 catgagacct tattatcata ttttcatgat ttggtattgt atcaatttca ttcacagtct
    27121 taagaccttt ttcttcatat tttgctactg cttgcttgtt atgaataaga ggacctaaag
    27181 catatatgcc agaatcattt ttttcaactt catcccatgc cattttcatt gctcttttta
    27241 ctccaaaaca aaagccagca ttttttgcta tttttatgtt cataacttta cccccttatt
    27301 ttgaatattt aaagtaattt tgttttatca cttctaaaat ctcttgtgat atcctttcat
    27361 ggtcatcatt actcaatttt tccttaaaat gttcttcaaa tgatattggt tctccaatat
    27421 atacaattac tttactaaaa agtttatatt tagatattat tgatactgga acaactttag
    27481 atttactttt tattgtaaat acagaaagac ctgccttagc cttaccaaaa gaatctcctt
    27541 ttattctagt accctctgga aatattccta atacataacc atctctaact gaccttaaaa
    27601 tagtttttat tgtagataca tctggttttt ctctatttat aggcataaca tgcaattttt
    27661 ttaatataaa tcccaatggc tttactttaa acaattcttt ctttgcaatt gcagctattt
    27721 ccctattttc tatggcagca gccaagaata tagggtcaag attcgattta tgatttgatg
    27781 ctattactat atttcctcta tctggaatat tctctgcacc tataacttca tatttaaaaa
    27841 atattttaga aaaaccctta aaaatattta ttacaaatct ataaaaattc acgtttatct
    27901 cccttcaaca ctacatttat tttcattatg atgcataaaa aactaaagat ttattttaga
    27961 tacaacttct tctacaactt cttctatagt ttttcctgta gtatctattt ctagtgcatc
    28021 attagctttt accaatggtg caaattctct atttgaatca atttcatctc gtcttattat
    28081 atcctcaatt acttcttcta aagtagtatt atatccttta ttacaaagtt ctttatagcg
    28141 tctatttcct ctttcttctg gagtagctac taaaaagaat ttgtaatctg catctgggaa
    28201 aacatatgaa cctatatctc taccatctag tattactgaa cttttcattc ctatttttcg
    28261 ttgaacttca accatcaact gtcttacttc ttttattttt gctacattag aaacattatt
    28321 gcttacttct attgttctta tttcttcatt tataacttct ttgtccaaat atatattgtt
    28381 atctttaaaa tctatatccg aattttcagc tatttgaata acttcttttt cattattgac
    28441 atcaacacca ctttttaaac acttataagt aactgctctg tacatagcac ctgtatctat
    28501 ataatttata tttagctttt ttgctactat cttggctatt gtactttttc cagctcctgc
    28561 tggaccatct acagctataa ctaaattacc catatataca cctctattta ttcatattta
    28621 attattaaaa aattatattt atacacttat acccgcaaga tatccagttg aaaatgctat
    28681 ttgtaaatta tatccaccag tttctgcatc tatatcaatt acttcacctg taaaaaataa
    28741 gttcttaact aatttagatt ccatagtaga agaattaatt tctttaactg aaataccacc
    28801 agaagtaacc ataccagtgt ttatacctgt agtcccatta caagttagct tcatattttt
    28861 tatatactca ataattctaa tttcatctgc cttagacaaa tcacttgcct ttttatcaac
    28921 taaagataat agagaaaaaa tctctcttaa gaaattttga ggtagtattt gcttaagatt
    28981 atttagaaca tttttatttg gaaatgctct gattatacta gagatttcat cagtacttaa
    29041 atttggtaag aaatctatat ttaattctac cttttccttt tcaataatct tgttaatata
    29101 agaagacatt ataagaacac aaggtcctgt tatcccaaaa tgggcaaata gcatgtctcc
    29161 agattttgat attttccttt tcttaatctt acaagatatt tccacattct tcatggatat
    29221 tccttgtaaa tctttaatcc atttttcctc tattgtaagt ggaactaaag caggatatag
    29281 tttgtttaat gtatgtccat gctttttaag aatcttatac attgaaccat ctgaacccgt
    29341 ctttgaataa ctcactcctc cagttgatat aataaccttg tctcctagta tcttatctcc
    29401 attatctaaa ataatccctt ttattagata ctttgacttg tccttattag aatcatcttt
    29461 tagagcaatt tcttcagtta taatatctat aactttctta ttatacatta tctttacatt
    29521 gtgattgagt aaatctttat ttaatacttc tattacttct atagatttat catttttagt
    29581 gtatactttg tggtcatttt ctttttcaat cttatattcc aaatttctac tttcaaaata
    29641 cgaaataagg tctttatttg taaatgtata gaaactacta tagagaaatt ttttattact
    29701 gacaacttta tcaaaaaaat cttctatctc tctattattg gtaaaattgc atctaccgcc
    29761 accagttgcc ctaagttttc ttcctaattc tccatttctc tcaactaata ttacttcatt
    29821 attgtttttt gaagctgaca aagctgccat cattccagaa gctcctccac caactactat
    29881 aactttactc ataaatttct ccttataatt gattcctact ccagtgcaga attatttatc
    29941 tttacatgat tttttatatg acttgtatca ttcattttta ctataacagg tccataatct
    30001 ttaaattcta ctatatttaa agcagtatta tcttgcttaa ttctatatat attttccata
    30061 cctgattcta aaaatgacaa caacataacc ctcaatgtaa ttgaatgtgt aacaagaatt
    30121 atatttttat tatcatattt ttcatttagt tcctttataa aagcatctac tctttccttt
    30181 ataatcttaa gagtttcgcc ttctggtata tttactaagt gtggttcatt tctccatgtt
    30241 gcatatatat ctgaataatc tttttgaatt tcttttatta aaaggccttc ccaaactccg
    30301 aatcccattt ctctaagtgc ctcagttttt tgaacttcaa tattgagctt atctcctaaa
    30361 atttgagctg tttgcattgc tctcccaagg tcgctactaa aaatataatc aattgaatac
    30421 ttgccaatat cttcagaaag ttcttttgcc tgttctatac cttgaggcgt aaggtcagaa
    30481 ttaccatgac cttgtgtttt tcccaatata ttccaatccg tttgtccatg tcttactata
    30541 taaaaagtat ttcccatatg tcctcctaat atatgcctat tttttattaa atataaatta
    30601 tcacttctca aaacttaatt atatcatata atactttact ttgtttctat tttataatct
    30661 aatctaacat attatttaca ataaatctca tttgtatttc tgcaaattat tgtactgtta
    30721 aagaattttt ctaaatttat aaacatattt ctcttatatt atttatatac ttcttcaaat
    30781 ctgtattctt gaattgaatt gacatactta tctgtatcta ctttttctaa aaacatatcc
    30841 aaaggtcttg caaatattcc tgtatcatca tataaggtct tgtataaaac taaatcatta
    30901 gtatcttctt cttttttatg ataaaggttt ccatctaaat ccctataaac atagatatct
    30961 tcttctcttt cagtatgtct aataactaat ttatatttat ttctgttttg gattaaattt
    31021 tctttaccat atatttcaca tatgttttct atatcatcaa tagccttgct aagacccatt
    31081 gtagcataat atttcccttt aaaatgttta tagatacaag gatattttaa ctttctttca
    31141 ctgataccta agttattctt taattcaatc attttattat aatctcccat atctcgtccc
    31201 tttcttttaa aaagtatttt attctgaaat aagttacttg ttttatattt tatcataatt
    31261 taaaatttat taataataca atcaataaat aaagaggctg tataaaatac agcctcccaa
    31321 tattaatctt cataatcata taatttttct aatttctttt ttaatttctt ttttctttct
    31381 gaacctaaaa ttcttataaa attattattc acaaaaatac aattattgct gagataaaat
    31441 tcacacttgt ctagatttac aatataacta ttactgcatt tgaaaaaatt ttcttctttc
    31501 aatagttttc ttatacaagg agcattttct ccaacttcat aattaccact ttcagtataa
    31561 atctttactt catactcttg cttttctatg tacaaaactt ctttactatt aattgaaaaa
    31621 cgtttatcct cgtattcaac attaataaaa ttttcctctt tcatttccat ccctccaagt
    31681 acttttatta atttaatttt agtgtttttt atcaatataa tctatcggaa atatatgtaa
    31741 gactgattta cttaagatat ttaaataatt attattaata ttaatgtata agtttatttt
    31801 ttaacattaa tttttaaagt taaaaagact tttt
//
LOCUS       DAMKPG010000037        31727 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMKPG010000037 DAMKPG010000000
VERSION     DAMKPG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.040"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            184..501
                     /gene="flgB"
                     /locus_tag="PC621_001848"
     CDS             184..501
                     /gene="flgB"
                     /locus_tag="PC621_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF4109007.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            509..913
                     /gene="flgC"
                     /locus_tag="PC621_001849"
     CDS             509..913
                     /gene="flgC"
                     /locus_tag="PC621_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF4109008.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            923..1240
                     /gene="fliE"
                     /locus_tag="PC621_001850"
     CDS             923..1240
                     /gene="fliE"
                     /locus_tag="PC621_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF4109009.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1265..2815
                     /gene="fliF"
                     /locus_tag="PC621_001851"
     CDS             1265..2815
                     /gene="fliF"
                     /locus_tag="PC621_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF4109010.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2828..3898
                     /gene="fliG"
                     /locus_tag="PC621_001852"
     CDS             2828..3898
                     /gene="fliG"
                     /locus_tag="PC621_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF4109011.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3891..4622
                     /locus_tag="PC621_001853"
     CDS             3891..4622
                     /locus_tag="PC621_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF4109012.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4626..5945
                     /gene="fliI"
                     /locus_tag="PC621_001854"
     CDS             4626..5945
                     /gene="fliI"
                     /locus_tag="PC621_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF4109013.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5977..6423
                     /gene="fliJ"
                     /locus_tag="PC621_001855"
     CDS             5977..6423
                     /gene="fliJ"
                     /locus_tag="PC621_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF4109014.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6451..7656
                     /locus_tag="PC621_001856"
     CDS             6451..7656
                     /locus_tag="PC621_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF4109015.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7674..8282
                     /locus_tag="PC621_001857"
     CDS             7674..8282
                     /locus_tag="PC621_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF4109016.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8309..9292
                     /locus_tag="PC621_001858"
     CDS             8309..9292
                     /locus_tag="PC621_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF4109017.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9305..9508
                     /locus_tag="PC621_001859"
     CDS             9305..9508
                     /locus_tag="PC621_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF4109018.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9508..10326
                     /locus_tag="PC621_001860"
     CDS             9508..10326
                     /locus_tag="PC621_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF4109019.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10316..11011
                     /locus_tag="PC621_001861"
     CDS             10316..11011
                     /locus_tag="PC621_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF4109020.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11023..11466
                     /locus_tag="PC621_001862"
     CDS             11023..11466
                     /locus_tag="PC621_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF4109021.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11481..11855
                     /locus_tag="PC621_001863"
     CDS             11481..11855
                     /locus_tag="PC621_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HDF4109022.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11856..12521
                     /gene="fliP"
                     /locus_tag="PC621_001864"
     CDS             11856..12521
                     /gene="fliP"
                     /locus_tag="PC621_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF4109023.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12534..12803
                     /gene="fliQ"
                     /locus_tag="PC621_001865"
     CDS             12534..12803
                     /gene="fliQ"
                     /locus_tag="PC621_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF4109024.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12815..14629
                     /locus_tag="PC621_001866"
     CDS             12815..14629
                     /locus_tag="PC621_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF4109025.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14638..16713
                     /gene="flhA"
                     /locus_tag="PC621_001867"
     CDS             14638..16713
                     /gene="flhA"
                     /locus_tag="PC621_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF4109026.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16688..17704
                     /gene="flhF"
                     /locus_tag="PC621_001868"
     CDS             16688..17704
                     /gene="flhF"
                     /locus_tag="PC621_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF4109027.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17701..18579
                     /locus_tag="PC621_001869"
     CDS             17701..18579
                     /locus_tag="PC621_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF4109028.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18593..19294
                     /locus_tag="PC621_001870"
     CDS             18593..19294
                     /locus_tag="PC621_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF4109029.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19310..19732
                     /locus_tag="PC621_001871"
     CDS             19310..19732
                     /locus_tag="PC621_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF4109030.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19717..19902
                     /locus_tag="PC621_001872"
     CDS             19717..19902
                     /locus_tag="PC621_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF4109031.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19943..20716
                     /gene="flgG"
                     /locus_tag="PC621_001873"
     CDS             19943..20716
                     /gene="flgG"
                     /locus_tag="PC621_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF4109032.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20737..21498
                     /gene="flgG"
                     /locus_tag="PC621_001874"
     CDS             20737..21498
                     /gene="flgG"
                     /locus_tag="PC621_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF4109033.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21521..22402
                     /locus_tag="PC621_001875"
     CDS             21521..22402
                     /locus_tag="PC621_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF4109034.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22405..22779
                     /locus_tag="PC621_001876"
     CDS             22405..22779
                     /locus_tag="PC621_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF4109035.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22811..24214
                     /locus_tag="PC621_001877"
     CDS             22811..24214
                     /locus_tag="PC621_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF4109036.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNVKVDDYKNEFLHKSSSNHKIND
                     YNNVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEADVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24330..26267
                     /gene="htpG"
                     /locus_tag="PC621_001878"
     CDS             24330..26267
                     /gene="htpG"
                     /locus_tag="PC621_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF4109037.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26655..27785
                     /locus_tag="PC621_001879"
     CDS             26655..27785
                     /locus_tag="PC621_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF4109038.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28012..29034
                     /gene="splB"
                     /locus_tag="PC621_001880"
     CDS             28012..29034
                     /gene="splB"
                     /locus_tag="PC621_001880"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF4109039.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29216..30127
                     /locus_tag="PC621_001881"
     CDS             29216..30127
                     /locus_tag="PC621_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4109040.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30142..30558
                     /locus_tag="PC621_001882"
     CDS             30142..30558
                     /locus_tag="PC621_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF4109041.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30652..31044)
                     /locus_tag="PC621_001883"
     CDS             complement(30652..31044)
                     /locus_tag="PC621_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109042.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31185..31580
                     /locus_tag="PC621_001884"
     CDS             31185..31580
                     /locus_tag="PC621_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF4109043.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        12997 a         3370 c         5861 g         9499 t
ORIGIN      
        1 tatattttta ttaggattta cgaaaataat attagttttc ttaccaaagt gatacattat
       61 tatattaatg taatagaaat gtaattaatt tggatgaata aagaagtcat tttttgtaat
      121 atagcaactt cattttttat taataagcaa aataaataaa atatattttg aggaggagtg
      181 agtgtgaata catatgacat tataaaaatg ggattagatg caactaattt aagaagtcag
      241 acaatagcaa ataatatagc caatataaat actgaaggat ataagagaca ttatgtaact
      301 tttgaagaaa cattaaatga tgctatgcca aaaatagaag aaaaaacaga caattctaaa
      361 ggattaagag tggatggcaa taatgtagat atagaagaag aaaaagtaaa tcaagcaatg
      421 gcaacattac aatataatgc tttgataagt tttgcaaatg gaaaaatagc aatgactaaa
      481 tcaataataa gtgggaggta attaaaaggt gagtgtgttc agtggaatga gaatatctgc
      541 tagtggatta acagcagaaa gcttgaaaat ggatatcata tcgtccaatg tagctaatat
      601 aaatgccaca agaggtccaa atggagaagc atataggaga aaagttgttt catttgaaga
      661 aaactatgat aataaattag gtatgctagg agtaaaagtt acatctgttg aagatgataa
      721 atcacctctt aaagaagtat atgacccatc tcatccagat gcaaagccaa atggatatgt
      781 agaatatcca aatgtaaata tattggtaga aatgacagat ttaataacag cttcacgagc
      841 atatgattct aacgtagata cattgaatgc tcagaagtct atgatatcaa aagctttgga
      901 aataggaaag tagggataaa ttatgccaat tacacaaatt aacaatttaa tgcaaggtgc
      961 aaagttagat aatttgattg gagaaacaaa accattaact cttaataata aagaagaaaa
     1021 aaagaacttt gaagatgtaa taatagatac tatagataaa gttaattttc aacaagtgac
     1081 agcagatgat atgaaagaaa aatttataaa aggcgaagat gtttctatgc atgaagttat
     1141 gttaataggt caagaagcac agctatctat gcaatattta atagaagtta gaaataagat
     1201 tcatgatgca tatcaagaaa tgaataaggt acaaatttag ccttttgaac aggagtaata
     1261 atcaatgata gccagtttta aaaataagat taaagagcaa gttgctaaat ctaggacaaa
     1321 gataagttct ctaaaaaagg gaactaaaat agcattaggt ataggggtta ttgctatatg
     1381 tttagcgcta gtatttacag caatatctgc gagcaaaaat aagtatgcag tcttattttc
     1441 agaactagat gttcaagatg ctgcagatat atcagcagaa ttagagtcta aaaagataga
     1501 tactaaggta aagggaagta cgatttatgt acctagaaca gaagttgata agttaaggct
     1561 ggagctagca ccaagtctta caggtggttc taaaggattt gcactaatgg atgatagttc
     1621 atcaatagga cttactgatg aagagtttca gattaagaag caaagaatga tacaaggtga
     1681 aatagaaaaa acaataaaga gttttccaca agtggaagat actagagtac atataacatt
     1741 tggagagtca tcagtatttg agaaggaggc tattccaggg agtgcagcag tttatttgat
     1801 gttaaaacca gggacgactt tggaaaaaaa ccaaataaaa tctgttatgg cacttatatc
     1861 aggaagtgtt acaaatatac cagagaaaaa tgttcaagtc attgataaca aaatgaattt
     1921 attatctgaa ggtattttag aggatgaaga tgttgatgaa aataatcaac caacaaatag
     1981 tttgattaat gttgataaaa ataaggcatc tgaacaagca ttgaataaac aattagaaaa
     2041 gtctatttta gaaatgctag aaccaatatt tggtaaagga aaagtaaaag ccacagtaaa
     2101 tgctgattta agttttgata cagctgaaaa aactgagata aaaattgacc ctgataaagt
     2161 tgcaataaag gagacaaggt cgaaaaattc atctaaatct gctacaaata atgttcaagg
     2221 tgtagatgct aacatgaata accaaggaaa taacaatggt actaatacag aagatagttt
     2281 agatgagagt tttgagtatg aaacaggtaa gattgaaact cacactatag ttgcgcctgg
     2341 agaacttaat agattgacag cttctgttgc aatagatgga aaaatagcta aaggtgtaca
     2401 agctgatgta gaggatattg ttaataatgc tataggtatg gatggagcga ggggagatac
     2461 tcttagcgtt gtttcaatgg tatttgaccc agaagggaag gcagaagcta aacaagagct
     2521 agaagagatt aaaaaagaag gatttaaaaa gaacataata aaagcagtta ttggagtagt
     2581 ggcagtggta gcaattggag ctattggata cttaatatat aagaaaagaa gtgaacgaga
     2641 agatatggat tatgaagatg aagattatat ttctaaatct gattctgttg ataggaagga
     2701 taatcctcta ttaggagatg acccagaact gacattagag gaagctgtta aaatgtatgc
     2761 agaagaaaag ccagaccaag taacagagat aataaaaact tggttaaatg agtaaggggg
     2821 atagaaagtg tccacggaaa ttacaaatgt taatgcgttt gacttaagtg atataaatga
     2881 aagtgataag aaagagaaga tagaaggtga gggtttaaaa aaagctgctg tattattaat
     2941 gacattagga ccaaatgtgt ctagtgatat aataaaaagc ttgcctgata aacaagttca
     3001 aaaaataggt ttagaaattg caaatatttc ttcaatagct tcaaaagaaa gaagagaaat
     3061 cttaaaagag tttgtagaga taaatagggc aaaagattat gttatagaag gtgggcttga
     3121 atatgcaaga gagttgttaa gcaatgcttt aggaaatgct aaagcaaaca aattacttga
     3181 aggtatatca ataaatacaa gtacaaagcc ttttaaacat attataaaac tagatgtaaa
     3241 gaaaatatta gaaatactta gggatgaaag tgcacaaact atagctgttt tacttgcaca
     3301 tatggagcca gaaaatggag cagaagtttt atcatactta gattttgatt tacaaaatga
     3361 agtagctatg agaataagct caatgggttc aatatctcca gaagtaataa aggtaattga
     3421 aagttcatta gaagataaat tacactcaat aagtggtgat gattttaagg attatggagg
     3481 agtgtctacc ttagttcaaa tattgattaa ttctgataga aaaacagaaa agaatatttt
     3541 gggaactatt gaagaacaag acccggcttt agcagctcaa attaaatctc atatatttgt
     3601 ctttgaagat attgtcaagt tagatgatat aagtatacaa aaagtattaa aagaagtgac
     3661 tatgaaagat ttagtatatg ccttaaaggg tgtggatgat gagatttcag atgctatata
     3721 tagaaatcaa tcttctagag cagcagatgc acttaaagaa gaaatggaaa tggttggtgt
     3781 cataaaagtt acacaggttg aggctgctca acaaaagata gcaaatatag taagaaaact
     3841 tgaaagagaa ggtgccataa ctattgtaag gaattttggt ggtgactttg ttgaataata
     3901 agattattaa aagtaaaaaa gcatcatata aaggagtgct agatttaacc ataatagata
     3961 aagttagagt gggagaatca aatgtaaata aagaaaatga ttgtagattt agagatgagg
     4021 taataaaaga agctatagaa tctgctgaga taaaaaagaa tgagatatta gaagaagcaa
     4081 aaatgaatgc acaaaatata gaaaaacaag catatgaaaa agggtatagt caaggtcaaa
     4141 aaaatggata tgaagatgga tataaagagt catatgatga atatacccaa aaagctaaag
     4201 atgaagcaga ttttataaaa aatcaggcag acttaatact attacaagct aatgagaaga
     4261 tgactgagta cataaaagaa aaaaataaag agattgtaag acttgctatt aatatggcta
     4321 gttctgttct aaaaaagcat ttcgaagatg aagaatctat gagtgatttg ttaaagcaaa
     4381 ttattcaaga ttatgaagga agtagttcat ttatagtaag atgtaattct ttctataaag
     4441 ataatataga gaaagagttt gaattattca aagaggaaac atcactaaaa tcaagtgtat
     4501 ttgttattgc agatgatagt atagataaag gcaatgctat catccaaaag gaaaatggaa
     4561 gaataatagt tggtatcgat tgtgctatgg aaaaactaaa agaagaattg cttggtgaat
     4621 aaagtatgta taaaactgat atatctatag attttgacaa aataaataag aaaatagaaa
     4681 atttagaact cactatatca gaaggtagag taaagaagat aataggactt actgtagaag
     4741 ttgaaggaat aaaagctttt gttggggagt tatgtgttat atacaatcaa gtcaataaac
     4801 cagtgaattg tgaagtagtg ggttttaaag ataaagaagt catattaatg gcgttgggag
     4861 agctaacatt gatagctcct ggttgtaaag ttatttctaa gggaatacct ttaagtgtta
     4921 tgtgtagcga taatttacta ggtaaagtac ttgatggtct tggaaatcct atagacaact
     4981 cagatgctgt tttaggagat agatacaact tgaacaatga acctcctgac ccaatgaaga
     5041 gaaaaaagat acgaaatata atggaaacag gtgtaagagc aatagatgca tttactactt
     5101 gtggtgaagg acagagaata gggatatttg caggaagtgg agtaggtaag agtacgactc
     5161 taggtatgat tgctaggaat gcaaaagcag atgtaaatgt aattgccctt attggagaaa
     5221 gaggaagaga ggtactagat tttattgata aagatttagg tgaagaggga atgaaaaaat
     5281 ctgttgttgt atgtgcaaca tcagataaag ctcctttggt taggctaaaa ggtgctctta
     5341 ctgctactgc aatagcagag tattttagag accagggaaa aaaagtaata cttatgatgg
     5401 attcagtaac tagatttgct atggcacaaa gagaagttgg tatagcaata ggagaaccac
     5461 cagctcaaaa aggatataca ccatctgtat ttgctatttt accaaaactt atggagagaa
     5521 cagggacatc agataaaggt tctataactg cattttatac agtattagtt gatggagatg
     5581 actttaatga gccaatagca gatacaacaa gagggatact tgatggtcat atagttttat
     5641 ctagagactt ggcaaataag aatcattatc cttctataga tgtattgaac agtttaagca
     5701 gacttatgaa tgagattgct tcaaaagaag acataaaaat agcatctttt gcgagagata
     5761 tgcttgctga gtatagagaa gcagaagatt tgataaatat aggtgcatat gcttcaggta
     5821 caaacaaaaa aatagatgaa gcaatctact atcatgagca tattattaat tttttaaaac
     5881 aaggaataaa tgaaaagagt tcttttaatg aaactataag tagtttaaga agaatctttg
     5941 aataaacaat ttaaaaaaat atttgaatag gttgtgatgg aattggataa aaattttaag
     6001 tttagattac agaaagtttt agacttaaaa ataaaagatg aagaagagat aaaaatggaa
     6061 tttgctaaga ttcaacaaaa aaaaatagat atagagagca atttagaaaa cttagaaagt
     6121 aattacagca aatattctat aagtaaaaat aatgatagtg ttcaaaatca aaaaataact
     6181 attaactatt tgttagcttt aaacaactct ataatggatt tatcagaaga gctggataag
     6241 tcgacaaatg aacttgaaaa agctagaaag cagcttataa gcaaacagat agagagaaag
     6301 tcattagaaa aattaaaaga aaaaaaatat ggacagtatt ataaagaaga acagttaaaa
     6361 gaacaaagca ctaatgatga gtttgcaagc atgagttatt tgagaaatag acaagttcta
     6421 taaaatagta ggaagtgagg atattattgt atgaagatac aagcagattc ttataatatt
     6481 attaaagata ttgggaatag aaaaagctca tctaagaata aaaatataaa aaataataaa
     6541 tttgaagata atctatataa ggcaacagac cataaaaagg ttaaagatag aagagaaaca
     6601 agtaagcaaa atgttagttc agataaacgg gaaaatagtg ataatgagtg tcatttagat
     6661 gaagataaaa gtaatcactt agaagatgaa aatacatatg aaaaagattg tagtaataat
     6721 aatgaagctt tacatcaatt actgaatctt ttaaaagata attacaaaaa agataaagat
     6781 ataaacacta tattgaataa tctaaaaaaa ctaagtatat cagatgaatt aaaacaagaa
     6841 cttaagctta ataaaaatat attaaataag attgcaaatc aaaatttaga gttaaataga
     6901 gctagtacaa ataaccctaa tgatatagaa aatttagaca taatgaagtt aagagaactt
     6961 ttagaaaata atgctagtga agatattgaa gtaattagta aaactacaga aaatgatact
     7021 aaaagcatag acttgtacag ttttgacagc aatagaatag atactcttaa ctcaaataaa
     7081 cagaaagatg atagttctga tattttggaa aaactagcag gggtaaatgg aaataagagt
     7141 ggaaattttg gacaagtaat aaataaaagc gttgatatga ataaaattaa aaatgataat
     7201 gttaatttaa aacagataga atcaaatgta atggatgatt ctataaaagc tattaagcat
     7261 atgaaaacaa atgatataca agaattgaca attaaattaa gaccaaaaga attaggagat
     7321 atgaacatac aattgttaaa agatggtgaa agtatgagag ctgttgtgac tgttttcaac
     7381 aaagatgtct ttgactctat aaataaaaac atagcagatt taaagcaaca tcttgaactt
     7441 acaaatgtaa atataaaaga tgtatcagtg caaatgcata gcgataatac aaatacatct
     7501 gatacctttg ataaagcatt tgaacaacag aatagacaaa ataaccaaga aaacatgagt
     7561 aagcaaaata caaatgataa gcgtaacaat gtagtcatag aagatgagat taaagaaaat
     7621 attattgatg atgatagagt agatttacta gcttagtaag ggggaagtat tgcatgtacg
     7681 gaatagcaac ggctttggca ggaactcagg taaactatag gtcaaataat ttaagccaac
     7741 ttgaaagaaa tattaatgat attgccaata aacaagttag aagtaaagga aatcagacag
     7801 taacagacaa tgggactcca attataggac aaggcgatga tgctgataaa gatttgttta
     7861 tgaagattct tgtagcacag atgggtaatc aagacccaat gaatccacaa gacccaacag
     7921 aatatgtatc acaattagca cagtttgcaa ctttagaaaa gatgtcagct atgaatgata
     7981 agctagatat acttttagcc ttaagcaatt ccactctaat aaattctgct ttgagtatgg
     8041 caacatcatt aattggtaaa gaagtagaag tatctgggga agaggaagaa gatgaaaaca
     8101 ttgtaggagt agtaaaatcc acatatataa aagatgggga agtacactta gaattagaag
     8161 tagatggaag tgacaaaact gtaggtgtta aatatgaaaa attacttaaa ataggagagg
     8221 tagatgcaaa ttcaggagaa gaaaatgact cttcagaaca agaatctcca gaagaagtat
     8281 aatattaaat gagaaaagag gtaaatttat gataaaagca atgtattcag gtgtaagtgg
     8341 aatgaaagca aatcaaacaa aactagatgt aataggtaac aatgtagcca acgctggaac
     8401 tacttctttt aagaaaagtt cagctagaat aacagattca ttttatcaaa ctgtactata
     8461 tgcaagtgct ccaacagcgg cattaggggg aacaaactta ggacaagtag gtgtaggtag
     8521 taagatatca agtataaata aagatatgac acaaggaaat gtacaaccaa ctaataggcc
     8581 atctgattta atgatagatg gagatggata tcttccagta gtaagacaag gaacagttat
     8641 gtatacgaga gatggttctt ttaatttaga tacaggattt aaagcagctg atggtgcttt
     8701 gaagaatatt aaaggtggaa gacttgtaaa tggtgatgga tatatgttac aaggtgtgat
     8761 ttatgatggg gaatataaag ctgatactgg agaattcatt aaaaagggtg atgctaaagg
     8821 aaaagagcct attgtaatac cacttcaaag tataagtcct aaggatggaa cagttcaaaa
     8881 tgtactagag tataatatat caaaggatgg aacagtagaa tttttattga gtgatggtca
     8941 aagaactcaa tttgtctatg atatacctga tggtgctaag gtaggagata cacctgctgg
     9001 aatagataaa ttaaaaggta caatacaaaa agtgcaaaca tatgcattcc aaaatccagc
     9061 aggactagat gcagaaggag gaaacttatt caagccatca gcaaactcag gagatccaca
     9121 aatagcagga gctacaggaa acataataca aggggcaata gaagcttcca atgtagatat
     9181 agcagaagaa tttacagaaa tgataatagc aagtaggtct ttccaagcaa acagtaaaat
     9241 aatatcgaca tcagacgaaa tacttcaaga aataataaat cttaagagat agtaggtaca
     9301 tattatgata agagtaacgg atttaaacgg aagaagatac atattaaatt cagaccttat
     9361 agtgagaatt gactctgtac cagaaagtag gattctcctt acaacaggag aaaaaaatct
     9421 tgtaaaagag tcagtagatg agatagtaga taaagtaata aagtataaaa gaaaagtaaa
     9481 tgtaggttat attaggagtg aaaaataatg aaaaaaacag attcactaac attcattgga
     9541 ctaatattat ctacagtatt agttttattt ggggcagtaa aaggaagtag ttcaggattg
     9601 aaaagttttt tcgattttgc atctatatta atcacagtat ttgggtcatt tggagctttg
     9661 atgattacat ttactattga tgatataaaa cttataaaag atgcgctaca atactcattc
     9721 aagacaatga atatatcgaa attagactta ttaaatcaat ttaaaacact ttctaaaaaa
     9781 gcaagaagag aaggactttt atctatagaa agtgatgtaa tgaaaataca agacccattt
     9841 atgaaaaaag ggttagagtt atgtattgat ggccttgaag taaatgaaat aaggtctata
     9901 ctagaactcg aaatagattc tgttgaagaa accaataaca gatattctaa actatttaaa
     9961 acttggggga ctttcgcacc tgcatttggt atgttgggaa ctctaatagg tttaatacaa
    10021 atgttatctg accttacatc tccagattta atagcatcgg gtatgggaaa agcattaatt
    10081 acgacatttt atggttcttt acttgctaat atcgctttga acccaattgc atataatata
    10141 gatgagaaga ctgaaaaaga gatttatgta aaggaaatga tgctggaagg tataataagc
    10201 atacaatctg gggagagcag catagtagtt gaggaacgtt tagctacata cctttcaaac
    10261 aatgagaagc tagaaattat gaaatccaat aaaaatacag aaagagcaat gagtaatggc
    10321 gcgtaaaaaa aataagaaaa aggatgagat aaatccagat gcatggttgg ctacttatgc
    10381 agatactata acactgattc ttacattttt tgtgttattg tatgctacat cagttgtaga
    10441 tatggaaaaa tttaaaaaca tagcagatgc gttaaaaggt cagttttctg ggataagtat
    10501 gttcagtaca ggttcaagta caagtgagaa gtctccaata gagagtgttt tagtggataa
    10561 aaatgataag tcaaatttag aacaggattt aaaagataaa gttaataatt ctaacttaca
    10621 aaatagtgta acagtcaaag atgatagtag agggatttta ctagaactag atgattcaat
    10681 tttatttgac tctgggattt ctgaattaaa agcagatagt aaagccactc ttaataaagt
    10741 atatgacatg ataaagatga tgaaaaataa gatagttata gaaggccata ctgataatgt
    10801 acctgtaaaa tcaagtacac atgaatccaa ttgggattta tcttctagta gagcagtaag
    10861 tgttgtaaga tactttgtag aagaaaaggg catggaccct agactatttt cggcaacagg
    10921 atgtggagag tatcaacctt tggtttcaaa tgaaacagca aagggaaggg ctgagaatag
    10981 aagagttaat atacttataa ctattgacta ggaggaagag gtttgttcaa aaataaaaaa
    11041 attattataa ttattttgat actgattatt ttctcagcag gagcaggttt ttttatatta
    11101 ggagaaggtg gaaataaaca aattgacaag attgcaggtt tgtttattcc aacagggaaa
    11161 ggcaaagatg aagtaaagac cgaaaatctt gacttagaag agatggtatt aaagctagct
    11221 gatgaaggtg taagatattt aaaagtatca ataactgtat cttatgattc ttcatataaa
    11281 gatatagaaa aaaatacgcc tttaattagg gataatatta taaattgttt tatggcaaaa
    11341 agggcagatg attttactgc tgataattta aagaacataa aagaagaaat taaaaataca
    11401 ttaaatatac aactaggtga aaatgtaatt caaaatatat atttcacaaa tatagtagtg
    11461 caataaaagg atggtaagac atgatacttt atttactaaa aatattaata tgtatcccaa
    11521 taattttaat attgatagtc ttatctttaa aaattagcca aggaacttta ttaaaaaaat
    11581 ataatgataa atatataaaa atactagata ttgtaaatat aagtaaaaac aatagcataa
    11641 taattttaaa aataggtgaa gaaggatgca ttatggcatc ttcttcctct agcatgaata
    11701 aaataaaaga tttgaccaaa gaagaaatat taagaataga aaacgacatt aaagaaagaa
    11761 attttaaaat tcaagatttt aaaatggaaa gaattaaaaa gaaatatgat ttcagtagat
    11821 atattggaaa atttacaaaa aaggaagata agtaaatgga actattatca aaattacctt
    11881 tggctgatgt accatataca agttcaatgc aattgttttt atttctaact gcattaatgt
    11941 ttttaccatt tgtaatgttt atgatgacaa gctttgtcag aatagttata agtttatcat
    12001 ttttgaaatc agcattagga gcacagcaag caattccaag ccaaattttg gtagggcttg
    12061 caatagtatt gacaatattt ataatgagac cagtattaaa tgagataaac gaaaaggcat
    12121 tacaaccata tatgaaagaa gaagttacta tggaagaagc tatgaaggaa gcagagggac
    12181 ctattaaaga atttttatta aagcaaacca gacagacaga tttagattta tttgtagaac
    12241 aggctggttt gaaagaaaag aaattaactc gtgaaaatat accactatct gttgtagttc
    12301 cagcatttgc tataagtgaa ctaaaaactg cttttcaaat aggtttttta atatatatac
    12361 catttttgat aatagacctt gttgtggcaa gtgttttgat gtctatgggt atgtttatgt
    12421 tgcctccagt catgatatca ttaccgttta agttattatt atttgtaatg gtggatggat
    12481 ggaacttgat agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg
    12541 aatttatgat aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc
    12601 cagtactcat tgtttctctt gtagttgggc ttttaataag tgtgtttcaa gctacaactc
    12661 aaatccaaga gcaaactttg acttttgtac caaaactaat agcaattctt ttagtagtag
    12721 ctattggtgg aagctggatg ttacatactc ttattagctt tacttcggag atattcaata
    12781 tgatgtctct tataactaaa taagtaggat atatatgatt tttgtattta ttagaatagt
    12841 ggcattcttt ggaacactaa ggatactatt tccagctggt acacctgctt tatttaagtc
    12901 gctctttgca atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac
    12961 aaatatagac aacattgtat tgtttacctt atatggtata aatgagacca taactggcat
    13021 tatgcttgga tatataacca atctatgttt ttatagtatc agaatggcag gttctatgat
    13081 ggatcaacag atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac
    13141 aactttaatt gataatctta tgaactggac agcacgtatg atatttttta gcatggatgg
    13201 tcatcatgtt ttaattagag gcataaggta tagttttgag ttaattccaa tagggaaatc
    13261 attcgtagat aataatatag attatataat aaacatattt gtgcagtgct ttttaactgg
    13321 attcaaaata gcaataccta ttgtactttg tctgcttatg gcagatttta tattagggct
    13381 tatatcaaga agtgtacctc aactaaatgt aatgataatt ggtatgccat taaaaatact
    13441 ggttggtatt gcgttattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt
    13501 attaagtcaa ataccaaaga tgtatgaggg aactttcgca ctagctccta tgttttttat
    13561 gggttcaaca gataaaacag aagaggctac tccaaagaaa aagggagaac aaagaaaaaa
    13621 aggtaatata gcaaagagta aagaattacc cgttgcaatg actctacttg cattcacttt
    13681 attagtacct acattatttt catatgtagt ggatacactt aaatcttctc ttaattattt
    13741 tttgagtcta gatttttata tgaatataaa ttattcaagt atagagaaat tagttatagc
    13801 aggattaatg gattttttta agatattttt accaatagct attccattct tagttttagg
    13861 tatagtcgca aacctatttc aagtgggaat actgtttact ggtgaaactt taaaacccaa
    13921 tttatcaaaa ttaaatccag ttagtggatt taaaaacatg ttttctatga gaagtttgtc
    13981 aaccttaata aaggatacag cgattatatc tattttggct tacataggat atacattctt
    14041 ccaagataat tatttagata tattaaagct aggtaacata tacctgccaa ctttaatgta
    14101 cacagttaaa gatttagtat attcaatatt aagtaagatt tgtgtagcta tgatagttat
    14161 agcagttgca gattatgttt atcaaagata cagtcataaa aagcaactta gaatgacaaa
    14221 acaagaagtt aaagatgaat ataaaaactc agaaggagac ccagaaataa aagctaagat
    14281 aaaacaaaaa caaagacaaa tttcgtctca aagaaccatg caagcagtac caagtgctac
    14341 tgtaatagta accaatccca cccacttatc tatagcagtg agatatgaaa aagggaaaga
    14401 ccaagctcca gtagtggtgg caaaaggtgc agattattta gcttttaaaa taagggaaat
    14461 tgcaaaggga aatgacatac ctataataga aaataaacct attgctaggt tgttatataa
    14521 gcaagtagaa atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt
    14581 agtagcagta tataaaatca aaaatagata caaagtcctc aaaaggtagg ataagtaatg
    14641 aataagttta atttaaagga aaatatggac gtaatagtgg catttggtgt tgtagggatt
    14701 gttcttatga tgatagtgcc gttacctaca tttatattag atattttact ggcaattaac
    14761 ataagtttat ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta
    14821 tctatatttc caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca
    14881 tcaactagat tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt
    14941 tttgttgtag gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt
    15001 caatttgttg ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg
    15061 ttagatgcca tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata
    15121 gatgacaaaa ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggc
    15181 tctatggatg gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca
    15241 gcaataaaca taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg
    15301 ggtgcagcac agacatatgt aagacttact ataggggatg gtcttgtaag ccagatacct
    15361 gctctattaa tatctacatc agcgggtata atcgtaacaa gagcttctac tgatgaaaac
    15421 tttggtggat tagtaagtaa acagttaacg gcaataccaa aagcagtaat gatgactggt
    15481 tgtgttctaa tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta
    15541 ggtataggtg ctatagcagc aggttacttt ttaggaagag aaaagaaaga tactttagaa
    15601 gacttggagt ttgaaaaacc tgatatggta aatccagata aaaatgaagt agaagatgta
    15661 acaaacttaa taaatgtaga atcaatagaa gtagaaattg gatatggttt aatatcttta
    15721 gctgatgaat cagcaggagg agatttactt cagagaatag catcaataag aagacaatgt
    15781 gcaatagaca tgggtatagt cgtgcaaccc ataagaataa gagataattt acagttaaat
    15841 ccaaaccaat acaatataaa aataaaggga aatgtagttg gtgggtatga gataatgcct
    15901 aatatggttt tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca
    15961 attgagccta cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca
    16021 gagataaagg gttatacagt tgtagattca tccacaatac ttataactca cttactagat
    16081 ataataaaat caaatgcaca cgaattactg ggaagacaag aagttaagac tattatagat
    16141 gcagcaagag aaaattatag tgctgtagta gatgaattga taccagattt aatgacactt
    16201 ggtgaaattc aaaaagtact taaaaatctg ttaagagaaa aagtaaatat aaaggatagg
    16261 gttacaatac ttgaaacgct tgcagaccag tctagaaata caaaagatat agagcttctt
    16321 actgaatatg taagaatagc cttggctaga agtatttgta ataatttagt tgatgatgat
    16381 aagacaattg tagtggctac attgtctcca gaaactgaag agttggtatc taataattta
    16441 caaaggtctg taaatggaac ttatccagca gttgacccag aaaatacaaa taaaatattt
    16501 gagagtatac aagaagtaat gaacaatgta tatttcaatc ataatatacc agtaatagta
    16561 gtatcaccaa agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg
    16621 actgtattat cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg
    16681 agcatatatg atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc
    16741 caaaatggaa ttgggtgaca atattacatt aatagataaa aaagaagtaa gaaagtctgg
    16801 aatacaaggt atcttttcaa agaaagatat tgaactaact attggatggg aaaagagaga
    16861 aaacgtagaa caaaaagatt taaaacgaga gatagaacaa ttaaagttaa ttattaacaa
    16921 tatggggttt gataataaga atgataatga tatagataaa atatgtaaaa atctactaag
    16981 tttagaatta aatgaagaaa ttgtagaatc tataagaatt gatttgcaag aaatgaagtt
    17041 caatggaatt gatacaagca aaaatttagt agaaatttta aagaaaaaga ttaaaataga
    17101 aaatcaagca ataaatggta aaattgctct agtcggacca ccaggagtag gtaaaacgac
    17161 tacaatagca aagttagccg caaaattagt ttttgaagaa aataaaaaag taggagtaat
    17221 aactatagat acatacagga ttggtgctgt agaacagttg aagatatata ctgatataat
    17281 gaatattcct tttaaagggg taataagtcc cgatgaaatg gaattggcac ttgatgagat
    17341 gaaagattgt gatgttgtat taatagatac aacaggtaga ggctataaaa actctatgca
    17401 aatactagaa atcaaaaatc ttatagataa agctgaaact gataatatac atttagttgt
    17461 gaattgtaca acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa
    17521 ttttaaaagc ttaataatta caaagctaga tgaaacaata acatatggct caatatttaa
    17581 cataatgaat tatgcacaaa aaccaatatc atatattaca acaggacaaa atgttcctga
    17641 tgatattata aaacctaatg aagataagat tataaggtta ctattaggag ttgaaagtat
    17701 atgatagacc aagctcaaat tttgagaaaa atggcaatgg aaaaaagagg acttgatgag
    17761 tttatagtag agaatgagaa tgtacctaag ataattacaa tagcttctgg taaaggcggt
    17821 gttggaaaga gtaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa
    17881 gttcttatat tagatgcaga cataggaatg tctaatatag atataataat gggggtaaat
    17941 gtaaaaggga ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca
    18001 cagactaagt atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac
    18061 tttacagaag ctcaaagaaa taagtttata catagcatag aacagattca cgatgtggat
    18121 tttataataa ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt
    18181 agtactgaat ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt
    18241 ttattaaaag ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg
    18301 gtaattgatt tagaagaagc aaaatctaca tacaaaagaa taaaaatggt tgtggacaaa
    18361 tttctaaacc ttaatctaga attgtatgga tatataatag atgacaaaaa agtaagttta
    18421 tgtgtaaaaa aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt
    18481 attatagcca tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa
    18541 agttttctaa gaaagatgtt tagtatattt aaaagctaag gaggcgtagt taatgaatag
    18601 agaagaatta ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat
    18661 accagggaaa ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga
    18721 tgcaataaat aaatttgatg ctgaaaaggg ggcaaagttt tctagttact cttatataaa
    18781 aataaaatct gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct
    18841 aactaaagta aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga
    18901 gccaacttca tatgaaattg caaaagaatt aaatgtttca gaaaaagaac ttcatgatat
    18961 cgaaagtaat atagacatgt taaatattgt atcattaaac tatgtaatct ttgaagatac
    19021 aaatgaaact gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat
    19081 agaagaagaa gaaaagttag agattctatc taaagcaatt agtaatttaa atgaaagaga
    19141 aaaattaata ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt
    19201 gctaggtgtt tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag
    19261 aaacaagata aaagaactta aatattctat atagatggtg attgataaga tgatacaaat
    19321 tatattaata gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga
    19381 atcaaaaaat aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa
    19441 tgatagatta ataattgcag aaaaaagaag attcttttta gacaaagtac ttgctgaaat
    19501 aagaagtaat aatcatataa aagtttatga aaattataac ctaataaagg ctaatgaaga
    19561 aagaaaggaa aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc
    19621 tcttaaagtg aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct
    19681 agatattgga aagggggaac tgtcattaat aaggagttgt tacaaaatat aaggatatta
    19741 ttatgtgata aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgc
    19801 ataatgcttt ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg
    19861 ttaggcatga aatatacaag tgagatgaaa gttttagaat agatgatact gtttagacat
    19921 ttacaaaaaa ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat
    19981 aactaatcaa caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg
    20041 atataaaagt aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg agggttacaa
    20101 taactacgca aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc
    20161 tatggatgaa actatcacaa attttaatca aggacctata aaaactactg ataataaaat
    20221 ggatgtagct attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt
    20281 ttacactaga aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg
    20341 gtattatgta cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa
    20401 tcaaaaattt gaagtggcaa aagataatac aatatcacta gatggaaatg ctatgtataa
    20461 attcaatatt gtagatttta atgactacaa taacttaaaa aaagaaggag ataacttgta
    20521 ttctggagga ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc
    20581 ttctaatgta gatatggtat ctgaaattaa taatatgatg accatatcaa gagaatatga
    20641 ggcaaatcaa aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat
    20701 tggttcagta agataggtga taggaggtga atttttatgt acacaatgtt ttatactagt
    20761 aaaacagctt tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat
    20821 tctgattcaa caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta
    20881 ctaaatagac catcatatcc tacaaatggc aatgatatat cgactggaac aggagctaag
    20941 gcaacagctc ccatgagaat ttatgaacaa ggtgcattgg cccctacaga tagtaagagt
    21001 aatttggcga tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca
    21061 tatacaagaa atggtggatt taatatagat ggtttaggta agattgtaga tgataatgga
    21121 aatatattag atgttcaatt tgatgcagga tataactata ataatacgaa tatagatagc
    21181 acaaacttaa cagtttcaag aaatggcgag ttattcgtag ataataaaaa aataggaaaa
    21241 atcaatttat atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattc
    21301 gtcgcaactg atgcagcaca gattaaacaa gttgagaaag tagatatagt tcaaggctat
    21361 agagagaggt caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga
    21421 gcatttcaaa tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagctaat
    21481 aatttaagag ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta
    21541 cgattttggc aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt
    21601 agctgaattt tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag
    21661 tatgattgtt gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc
    21721 ttcatgtgta gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat
    21781 agatagaagt gtagctgata tgtggattga tataacttct ggaggaactg gagttataaa
    21841 ggataatgat agagagctta cagaacttga taaaaaagtg cttttacatc ttatggatga
    21901 tttagtaaga aatatgcact tatttgaagg ttttgaaaat atacaagtat tggatacaca
    21961 tacacatatt aatttaccac aattatgttc tccaactgca ccagtatgcg ttgtagattt
    22021 aaaagtgcta aatgataatg agtatattgg aagtgtttct ctttgttttc cgtataatgg
    22081 tttagaggct ctattagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga
    22141 tgattcagag gaatttacca aaaaaatcta taatagtgta tctaatatag aattatcagt
    22201 tattgcagag ataggaaaag tatcaattaa tgttgaagac ttacttaaac tagaagttgg
    22261 agatgtaata gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc
    22321 aaaatcatat acagcaacac caggatttat atcaagcaaa aaaggtgtaa aaataaatga
    22381 tgctgttgga aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca
    22441 aagatgttat taaatgataa agttgaaagt gaagttgatg gggatgatga gttagcctta
    22501 gatgtctctg aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta
    22561 tctgtagtta tagggagaac taagatgact ctaagagata ttttaaattt acataaaggt
    22621 tctcttattg aactgaatac tttagcagac caagatgtgg aaatactaat agataataag
    22681 gttttggctt atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt
    22741 atagtaagtg aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt
    22801 aaggcatgag gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact
    22861 caataaaaaa gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg
    22921 tatatcaact ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga
    22981 tatattaaat ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga
    23041 aagtttctat aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat
    23101 gcttacatct gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat
    23161 aaataaagga atgtataaag aagctataca agggtttaaa cttataactg accaatactg
    23221 tgatttaata gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat
    23281 aaaatttgat gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa
    23341 tcaagaaaat aacacattag atatgtataa tagtgctaat atacaaacta aaagtaatgt
    23401 aaaagtagat gattataaga atgagttttt acataaaagt agtagtaacc ataaaattaa
    23461 tgattataat aatgtaacta aagaaaaaag ttcaagcaaa aaccagaaaa gttcagattc
    23521 taataagaaa aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat
    23581 attattagta ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat
    23641 atctaaaaat aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga
    23701 aaataatata gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa
    23761 aaacattaat aaagaggctg atgtaaaatc taaaaaaaat actgtagaaa atgaagatga
    23821 taaagattct gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc
    23881 agaaaaaaaa actaaatctt ctatacaact atataagaaa gttgcagata taggtgaaaa
    23941 taaagggaat acatctgaag caacttatca agttgctatt ctcagtgaaa agttaaaaga
    24001 ctataaaact gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc
    24061 ttattttgat gaatcttatt ataatttagg gatgatgtac tataacaatg gtgatttaaa
    24121 aaattctaaa ttgacattaa agaagttagt taataaggta ccaaatagta tgtataataa
    24181 ttcaaaagta aaagaaattt taaaagaaga atagttaaat tagataagaa aaataaatta
    24241 ttttatttta aaataatatg attataatat attgacatac cattttaaaa aatttaaaat
    24301 attaattata aaatgaggag ggataaaaaa tggaatttga aaaagggagt atttcaattc
    24361 atacagaaaa tatttttcct ataattaaaa aatggttata ctctgataaa gacatattta
    24421 taagagagct tataagtaat ggatgtgatg cagtaagcaa acacaaaaga cttgtatcac
    24481 taggagaaat atcagaaaat aagtcatcag attataaaat tacagtatct gtaaataaag
    24541 gagaaggtac acttaaattt attgacaatg gaattggtat gactgaagaa gagataaaaa
    24601 aatacataaa tcaagttgct ttttcaggtg ctgaagattt ctttaataaa tataaggata
    24661 agatggaaga gtcaaatgac ataataggtc attttggatt aggattttat tctgcattta
    24721 tggtgtctaa aaaggtacaa atagatacac tttcatatac agaaggagcg actcctgtaa
    24781 gatggatatc tgaaggtggt acagaatacg aaatatctga atcagatgct agaaatgata
    24841 gaggaactac tataacacta tttattgatg atgatagtaa agaattttta gatgaattta
    24901 ctgtaagagg aataataaat aaatactgtt ctttcttacc agtagaaata tatttagaag
    24961 atgttgaaag attagaaaga gaagctaaag aagcagaaga aaaagctaaa aaacaaaagg
    25021 aagatggaaa agaagagatt gtagatgcaa aagtaattga gcctttaaac gacactaatc
    25081 ctctatggtt aaaatctcca aaagactgta ctgatgagga gtataaagag ttttatagaa
    25141 aggtattcaa tgtatttgat gaacctttat tttggataca tttaaatgta gattatcctt
    25201 tcaatttaaa gggtatactt tacttcccta aattaaaaaa tgagtttgaa ctgactgaag
    25261 gaaaagtaaa attatacaac aatcaagtgt ttgtggctga caatataaaa gaagttatac
    25321 cagaattttt acttttatta aaaggtgtta tagactgccc agacttacca ttaaatgtat
    25381 caagaagttt cttacaaaat gacagggatg taagcaaaat ttctaaacat ataattaaaa
    25441 aagtagctga taagttaaaa tctctttgta aaaatgaaag agaagaatac aataagtttt
    25501 gggatgatat acaaatattt attaaatatg gttgcttaaa agatgaaagt ttctatgaga
    25561 aggtaaaaga atgtatatta ttcaaaacta ttgatgatga gtatataaca atacaagatt
    25621 atttagaaaa atgtaaggac aaacatgaaa ataaggtatt ttatgtaagt gataaggaac
    25681 agcaatctca atacattaaa ctatttaaag aatacgactt aagtgctgtt gttttaaact
    25741 catcaataga tactcatttt atttcattta tggaatataa agagaatgga gttaagttta
    25801 atagaataga tgcagacctt tctgatgtat taaaagataa aaatgaaaat aaagatagtg
    25861 aagaaaacaa agaagagata gctaagatag aaggtttgtt taaagaagct gttggagaaa
    25921 gagtgaagaa ttattctgta gaaggattga aaaatgaaga cactccagct atggtattag
    25981 tatcagaaca atctattaga atggcagaaa tgcagtcaag atttgctgga atggatttag
    26041 gaatgaattt tgaggaagaa aaaactcttg taataaatga gaacagtcca ataatcaaaa
    26101 aattggtttc tcttaaagat gatgaggaaa agaaagataa gattacgctt atttgtaacc
    26161 agatagcaga tttagcttta ctttctaata aagaattaaa gccagatgag ttagattcat
    26221 ttgtacaaag aagtaataag cttatgagtc ttttgataga attataagat tgaatttgaa
    26281 tacattgaag caaatttata aaaagtattc aatatcagtg atgagaaaga agtgtctcaa
    26341 aatgaacttt tcagttcatg aggcgctttt tttatgtgaa attaaattta ttttaatcat
    26401 ttcaacaatg aaaaatatat ttatctctat tttgagacag accgttcttt atttttttat
    26461 ctttggttaa tttttaaata tttaagtagg ttctaataaa aaactctatt tagttaaata
    26521 tagttaactt gatatataat aagataatac atataattaa gtaaaagatt gttttttagt
    26581 ttatgtttaa atacataatt acactttgtt aaaaacataa aattattact aaagtagaaa
    26641 taggaggttt tagcatgaat tttaattata atttgccagt aaatatctta tttgggagag
    26701 gcagaattaa tgatgtggga aaagaagtat ctaaatatgg caaaaaagta ttgatagtaa
    26761 caggtaaaaa tagtacaaag aaaactggat tacttgataa aacaatagat ttactaaaag
    26821 actctaaaat agaatatgaa gtttttgata gagtagaaca aaatcctctt ataactacta
    26881 tatattcagg cgtagaaatt ataaaatcaa ctggatgtga ttgtgtttta gggcttggtg
    26941 gtggaagcat tatggatgct gccaagagta tagctttttc ttataaaaac ccaggagatt
    27001 taaatgatta tatatttgga attagacaag gtgaagaagc tttacctata atcttagttc
    27061 caacaacatc aggtacaggt agtgaaggaa attgttttgc agtacttaca aattcagaaa
    27121 ctaaagataa aaaatctata aagaagaata gtatgtatgc aaaagcatca ataattgacc
    27181 cagaacttat ggttacaatg ccaaagcata taattgcatc agttggattt gatgcacttg
    27241 cccataatat ggaagcatat ctttctaatg gaagaaaccc tcttgcagat gtacaagcta
    27301 tttatgggat tgaacttata tcagaaaacc taataaaggt atataatgat gttaatgatt
    27361 tagaagcatg ggaaaaaata acacttgcaa gtactattgg aggaatgacg attggtacag
    27421 cgggtacagc cttgccacat ggaatggagc atcctgtaag tggtcttaga gatgtaatgc
    27481 atggaaaagg acttgcagcg ttgactccta ctattataga aagttcatgg gatattgata
    27541 ttgaaagatt tggtaatatt tcaaaggtat taggaggaac ttgtgcagaa gattgttcag
    27601 atgcaattag aaatttcctt aaaaaaatag atttagatat aaaactaagt gatttaggta
    27661 ttgaaaaatc agatatagat tggttaactg ataattgtat gaaaatatca gttgcaaata
    27721 ttaagagaca tccaaagtat tttaataagg aacaaataaa ggaaatgtac cataagtctt
    27781 tataatttga ctttacatat ttagttttat ataaaaatta taaattaata ataaaaaatt
    27841 ataagtaatt aatagtaaaa aaattgtgta tagaagttat atatataata aacttgtctt
    27901 aagcttttat tttactttct aataaataaa ggagtttaaa aaagggaaga ttaattaagt
    27961 aatattttat aaaattcttc gatttaaaaa ctttaagtag aggtgaaata aatgtttaat
    28021 cccaaaagaa taatattcga aaaaggaact ttagacactg atgtgggcaa aaaaatatac
    28081 aataaattta gagataataa agatgtagag ataattaata tatcatcaaa taggattaaa
    28141 gaacacattc caggtgaaaa tctatatgaa caatatagaa gtgggaagca gactatggta
    28201 gttgggatta gaaaaagtct gaagtttcaa acatgtaaac cttctgctaa ttatcaacta
    28261 ccacttgtca gtggatgtat gggaagatgt gagtattgtt atttgaacac acaattaggt
    28321 gataaaccat ttataagggt gtttgtaaat gtagatgaaa tacttgaaca gacaaaaaaa
    28381 tatattgaag atagaaagcc acaggtaacc atatttgaag gggcagctac atcagaccca
    28441 gttccagtag agccatatac taattctttg aagaaaacaa tagagttttt tggaagagag
    28501 gaaaaaggaa gatttagatt tgttactaaa tacagtgatg tggatagctt attggatgct
    28561 gaacataata atcatacaga aatacgattt agtatcaata ctcctagaat tataaatgaa
    28621 catgagcatt atacagcctc tgcaaataaa agaatagagt cagctgtaaa acttgctagg
    28681 gctggttatc agattggttt cataatagca cctgtgtttt tatatgaagg ttggaaagaa
    28741 gagtataaaa atctgattat aagtataaaa gaagctttac caaaggattt tgataaaaaa
    28801 attatatttg aagttatatc tcatagatat acaactaaag caaaaaatag aatactagag
    28861 atttatccag atacactatt gccaatggaa aatgaggata gaaagtttaa atacggtcaa
    28921 tttggatatg gtaagtacgt gtatgataaa gaacaattag atgatatgaa agagttcttt
    28981 cttaaggaaa taaatggagt atttaaaaat agtttaataa aatatataat atagcaaatc
    29041 ttacctaaag atttgctata ttttattttt atatatttta tgtttataat aaagcaatga
    29101 gatttagcac tatcatataa gaaaattaaa aagggcacta tctatcaaga acattttttc
    29161 tagttttgat actataaagg gtaagagtaa caaaaatatc aggaggagtt atagtatgca
    29221 tgcatgggag tctattcaaa ttacattgga tttaatagaa gcaaatttat cagaagaaat
    29281 atcaattaat gaattagcaa ataaagcaaa tttatcacca ttttactatc aaagactatt
    29341 taaaagattg gtaaataaga ctgtaatgga gtatataaag cttcgtcgtc ttgcaagagc
    29401 ttctgaatac ttagcagaac ataagaatcg tatacttgat gtagctttaa attttggatt
    29461 tgctagtcat gagacattta cacgttcctt taaaaaagca tatggattaa ctccagaaaa
    29521 atatagagca aatccagtaa aactcaatca ctttattaag ccagaattaa ttttaaatta
    29581 tgctatggtt gatgaaggtg ttccactgat tgcagatgat atagtgattg aagtcaatcg
    29641 taagacattg agtaatccaa ggacatttgt tggaatagaa atagaagtac ctatttgcca
    29701 gttggtgggt ggtgaaacaa ctggaattgc aatagtagag gaattatgga tgaaacttgg
    29761 aagtcaaaga cataatatac caaatcaaat tccaggtgga aatgaatttg ctgctcttta
    29821 tatgggaaat gcaaaagaag gaaattgtat gtatatggca ggtgtagagg tagagtcagg
    29881 tacatcagta gaaggatata atacttttga gttgccagct aaagaatatt tagtatgtgg
    29941 atttgaggca gaaaacttta atgagttagt taattcagct gtttttaaag cagataaatt
    30001 tatggaaaga tggatgaaga aacataattt aacaactact gattttgcaa ttgagatgta
    30061 ttaccccact acacctgaag cagcatatct ggaacattgg attgttccgg ttcctattga
    30121 acaatagaaa ggatgatgaa tatgaattgg caagatgcgt tttttcaaga tactaaacct
    30181 acatttgagc aaattaatga atttattaat aatccattat gggacaagtt aaataaaact
    30241 ttggtttcta cttataatgt agaacctaag cttgaataca gtaaatgttc aatgcaaaga
    30301 ggttggaatg taaaatataa aaaaagaggt aaatcactat gtactttata tccacaagaa
    30361 ggttatttta aagctttggt tattgtaagt gaaagcaata gagtagaggt tgatttattt
    30421 attaatactt gttgtgatta tatcaaaaaa atatataatg aggtaaactt ttttaatgga
    30481 agtaaatggc taatgattca agtagatagt ttattggtat taaacgatat gcttgaacta
    30541 attaaattta gagcttaaca tatagaacag gatgaatata taagtcatcc tgtttttata
    30601 ttcaatcaga taaattaata tatagtctat aatatgaata atttagtaaa tttactttat
    30661 ctttctacat tctttgcaac taagtccaca tgtgtttttt tcttgcatat tagtagcacc
    30721 ccaattagca aattgttgta aaataggcac aatactttta cctttacttg ttaatgtata
    30781 ctcaactttt ggaggaattt cattatattg tttacgatga acaataccag tagactctaa
    30841 atcttttaat gattgagcta gcatagtatt tgtaatgcca tccaattttc tttttatgct
    30901 actatatcta attggatttt caactgctaa catacatata ataggtaatt tccacttacc
    30961 accgacaatg tctaatgcat aacggattgg acaggttaat tcttcaacgt ttttagttat
    31021 atttttttca gtatttatac tcattttaat tactccttag ttatagtagt atctttttta
    31081 tactttatca gaaaaaactg catacttgtt ttttctgtat tttatattaa aattatatca
    31141 aaggataatc cttaatacaa gtatttaaaa ggggtgtaag aattatgaaa gcattagaat
    31201 ttttgaacaa ggcaggagtt tattatttag caacagttga tgagaacaat caagcacatg
    31261 tgcgtccttt aggatttgtt atggaataca atggtaaact tacattttgc acatctaatc
    31321 aaaaggatat gtttaagcaa cttattttta atccaaaggt tgaactttgt tgtatagacc
    31381 aaaaccttaa tactctacgt attttaggtg aagcagtatt tgttacatca aaagaaacac
    31441 aagctaaggc acttgaaata atgccaagtt taggtcagat gtactctgtg ggagatggta
    31501 agtttgaaat attctctatt gataaggcaa aagttagttg ttgttcaatg agtggtaaaa
    31561 agattgatgt agagctttaa ttataattga atcgacatcc atagtgaaaa ttaagaaaac
    31621 gttattactg aaatattgat agatttaatt taagtgcaat aaaaagattt ttaaataatg
    31681 aatatgtaaa aatttttagt tattagatgt gtatttcaat aatgata
//
LOCUS       DAMKPG010000038        31350 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMKPG010000038 DAMKPG010000000
VERSION     DAMKPG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.041"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..1346)
                     /locus_tag="PC621_001885"
     CDS             complement(<1..1346)
                     /locus_tag="PC621_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4109044.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKI"
     gene            complement(1383..2603)
                     /locus_tag="PC621_001886"
     CDS             complement(1383..2603)
                     /locus_tag="PC621_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4109045.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            complement(2972..4984)
                     /locus_tag="PC621_001887"
     CDS             complement(2972..4984)
                     /locus_tag="PC621_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109046.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            5455..6105
                     /locus_tag="PC621_001888"
     CDS             5455..6105
                     /locus_tag="PC621_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF4109047.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(6245..7621)
                     /locus_tag="PC621_001889"
     CDS             complement(6245..7621)
                     /locus_tag="PC621_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4109048.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(7637..9550)
                     /locus_tag="PC621_001890"
     CDS             complement(7637..9550)
                     /locus_tag="PC621_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF4109049.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            9686..10318
                     /locus_tag="PC621_001891"
     CDS             9686..10318
                     /locus_tag="PC621_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF4109050.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            10457..10996
                     /locus_tag="PC621_001892"
     CDS             10457..10996
                     /locus_tag="PC621_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF4109051.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(11172..11342)
                     /locus_tag="PC621_001893"
     CDS             complement(11172..11342)
                     /locus_tag="PC621_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4109052.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            11627..12694
                     /locus_tag="PC621_001894"
     CDS             11627..12694
                     /locus_tag="PC621_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF4109053.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            12864..14765
                     /gene="cooS"
                     /locus_tag="PC621_001895"
     CDS             12864..14765
                     /gene="cooS"
                     /locus_tag="PC621_001895"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF4109054.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            14758..15231
                     /locus_tag="PC621_001896"
     CDS             14758..15231
                     /locus_tag="PC621_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF4109055.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            15206..16432
                     /locus_tag="PC621_001897"
     CDS             15206..16432
                     /locus_tag="PC621_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF4109056.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(16571..17758)
                     /locus_tag="PC621_001898"
     CDS             complement(16571..17758)
                     /locus_tag="PC621_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4109057.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            18031..18855
                     /locus_tag="PC621_001899"
     CDS             18031..18855
                     /locus_tag="PC621_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF4109058.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            18984..20249
                     /gene="gluD"
                     /locus_tag="PC621_001900"
     CDS             18984..20249
                     /gene="gluD"
                     /locus_tag="PC621_001900"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF4109059.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            20442..21098
                     /locus_tag="PC621_001901"
     CDS             20442..21098
                     /locus_tag="PC621_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF4109060.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
     gene            21429..22562
                     /locus_tag="PC621_001902"
     CDS             21429..22562
                     /locus_tag="PC621_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF4109061.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
     gene            23489..24511
                     /locus_tag="PC621_001903"
     CDS             23489..24511
                     /locus_tag="PC621_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF4109062.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            24807..25727
                     /gene="pyrB"
                     /locus_tag="PC621_001904"
     CDS             24807..25727
                     /gene="pyrB"
                     /locus_tag="PC621_001904"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF4109063.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            25766..26458
                     /locus_tag="PC621_001905"
     CDS             25766..26458
                     /locus_tag="PC621_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF4109064.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            26451..27353
                     /locus_tag="PC621_001906"
     CDS             26451..27353
                     /locus_tag="PC621_001906"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF4109065.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            27438..28022
                     /locus_tag="PC621_001907"
     CDS             27438..28022
                     /locus_tag="PC621_001907"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF4109066.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            28237..29163
                     /locus_tag="PC621_001908"
     CDS             28237..29163
                     /locus_tag="PC621_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HDF4109067.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(29215..30105)
                     /locus_tag="PC621_001909"
     CDS             complement(29215..30105)
                     /locus_tag="PC621_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4109068.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            30293..31324
                     /locus_tag="PC621_001910"
     CDS             30293..31324
                     /locus_tag="PC621_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF4109069.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
BASE COUNT        11814 a         3780 c         4974 g        10782 t
ORIGIN      
        1 tgaatttttt ctctcgaaat atataattta gaatcttctg atttattttt atgctcgatt
       61 tccttatttt ttttcataaa tatatataga ggagctatta acaacattgc ccctaatcct
      121 attaagactc tattttgagt tataggtttt gaaatatctt ctgcccaagc ttgttgtaac
      181 aaccataagc tacctaaaat agctaccatt ggtataacag gaccaaatgg aatcttaaaa
      241 gaggccttaa catctgttct ttttcttaaa actattactg ataaacaagt tggtatgtac
      301 tgagcaaatc ttgcaattac actcattact gcaagttgtt caaaacttcc agttagcaca
      361 agtggtatac aacaaattaa agatattatt attgctacat atggagcacc atatttattt
      421 gtttttccta taaatgcagg aagagaacct tcttctgcta gagaagaacc acatttagga
      481 gttactattg atgaacctat atttatacca aatatagata ttaatgttgc tacagatatg
      541 aaaactttac cataattgcc taaaaataca cttgaagcat ctgcaattgg aatactgttt
      601 tcaaatagtc tatctccaag tatacccata cagacaattt gaattaatat ataaattatt
      661 gcacatataa atattgtggt tattaatgcg attggaagat tttttttagg attttccatt
      721 tctccacttg ccacaataaa tgattcaaag ccggtaaatg cgtaaaatac taatataatg
      781 gctggaccca tactagagtt aactacattt cctgaaggaa caatatttac aaattttata
      841 aagaatattc caactatcac aaaaactatt aatggtacta atttagctat agttattaca
      901 ttattcatta tctttgtgct tttaattcca aataagctat ttattgaaag tagtgttacg
      961 agtattgcag ctatacaacc tttgtattca gtggcagatt ctggccaaaa tgaacctaaa
     1021 gctgttgcaa atcctactgc tagagtagac catgatataa ttctaataaa ccaagacatt
     1081 gtaccaactt caaatcctat aaaatctcca aaagcttttt tggagtatag atatgctcct
     1141 ccattcttat caaacatgct tccaacttct gcaaaacata aaagtattga tagaactaag
     1201 agtgtagcaa aaatgtatat aaacatactg ttttgacctg ctagactata aactttacct
     1261 ggtagtaaga atatacctga acctattatg ctattaatcc ctaataatat catactaaat
     1321 agacctaatt tcttagcttt ttccatatta atttatctcc tattctttta ttaattactg
     1381 tactaattaa aatacaaagt attcattaca tgcatcataa cacctattgg taaacaatct
     1441 tcatctatat tgaagctaga ggtatgaaga ggtgaaatta agcccttttc ctcattttta
     1501 catcctaaat ggaagaatgc tcctttacaa tgttctgtgt aaaaagaaaa gtcttcacca
     1561 cctaaagacg ggtttggtct caatataaat ttttcctctc caagtaattc tttagtactt
     1621 gatataactg tatctactaa ttctttttca tttataacag caggatagtt ttcatcactt
     1681 acatgtaaag tcccaacaca accaaatgca gatgcagtgt ctccaacaat tttagctatt
     1741 ttatcaatca taaagtttct tgttttgcta tttaaggttc ttaaagtgcc ttctagtttt
     1801 acatcttcac atattacatt gtgtgcatca ccaccatata ttttacctat agtcaaaacg
     1861 gcagcattat ttggttctaa gtttctactt attatagttt gtaatgatgt aacaatttgt
     1921 gaagctgtaa ctattgcatc aataccattt tcaggatatg caccatgagc tcttttacct
     1981 ttgatactta tttttatagt gtctacacta gcatttaatg tatcatattt tgtttcgata
     2041 aggcctgttt ctatatgtgg cataacatga agtccgaata tataatcggc cttggggttt
     2101 tctaaacacc catcctcaac taaaaatttt gctcctccaa aaccttcttc tgctggttga
     2161 aataagaatt ttacatttac attaagttta tcttttattg aatgtaaaac tttacatgct
     2221 ccaagtagca tggttgtatg tgcatcgtga ccacaagcat gcatttttcc agaatgtatt
     2281 gatttataag gtatgttgtt ttcttcttca ataggaagag catccatatc tgctcttata
     2341 caaatcgttt tatctgcact ttcttttaaa atatatgcca taattccatt gtgttttgta
     2401 aattccatat aatcaattcc gatttctttt aaataagaaa ttaccttctg tttagtaaga
     2461 ttttcttcaa gagctaattc tggaatttta tgtaattctc ttcttatgtt tataagccat
     2521 ggttttatag aattacaact ttctattaca aaatcttttc ctaattcttt ggtttgatat
     2581 tttaatttca aagcctgttg catttttatt cctccaattg ctttttattg ttttattaat
     2641 ataataagca attattatgc cataattttc gatggagaat tttgtttttt ataagaatat
     2701 tgaataaata taatcattta tctatatgta tagtataaaa aatgattgca tagtttcaat
     2761 tttcagtaaa aaatattatt ttgaagaaaa tatattttat atgctagttt atataaaaaa
     2821 aataggaaat aaaaaaatta tcctattcct attaattcct attatttatg tacaaaataa
     2881 attataattt tagggtactt gaaaaataaa aaaacctatt gaaggagtac tttaatcctg
     2941 aatagatttc tttgtttgat aaattataat tttatagacc tttatatttt aatttattgt
     3001 aaaaatcttc tcctttttga gttattttac ttccatatct tcctgaacta caaattataa
     3061 gttcttcttt ttttagatat tcaaatattt ttttaatttt tccttctgta atttgaaggt
     3121 tcttatcgag tagtgctttt aaaattaatc ccctaccaat accaacatct gattccttat
     3181 ttaaaattag tatctctaat atattcaata tatcattctt actatatttt agttttaatg
     3241 atgtattttt gttatctgaa gaccttaagt ttggaggtaa atctttctct aatacaatat
     3301 cttcacacat aatgtttaaa taggatgtaa cattttggag ttctcttata ttacctttcc
     3361 acatatagtt ttgtataagt ttttttgctt ctggtgtgat tactaatttc ttattaatta
     3421 aatcttccat aataattaat atgtcttctt ttctttctct tagaggaggt atatttattg
     3481 gaattgtatt taatcgataa tataaatcct ctctaaattg gctattatca atcatttgtt
     3541 ctaaattctt atttgtagca gatatgattc taacatctat attaattaca ttgtgagacc
     3601 caacaggcat tatctgcttc tcttgaagaa ctctaagtag ttttgtttgt aattctaaag
     3661 gcatatcgcc tatttcatct aagaatattg ttccattatt agcaagttca aataatcctt
     3721 gttttccatc ttttagacct ccagtaaaag ttcccttgtc atatccgaat agctggcttt
     3781 ctaataaatt ttcaggaacg gctgcacaat ttactgctat aaaaggttga ttttttctat
     3841 ttgagttgtt atgtatagac tgagctatga gttctttacc agttccactt tcacctctga
     3901 taagtatgct taagtcagat ttagaaacct tcttagctaa atctatacat tcaaacattt
     3961 taggactatt tgtttttata tccttaaatg tatattttgc tatttgtcct ttttctctta
     4021 attttttagt gagattttgt tctaattttt ttatgtatgt tatttcttgt aaactataat
     4081 atgtgcccat tttttcatcc ctattgtata tgtttttttt atttacattt atatatttct
     4141 tattgaatac gacaacttta tctagtatct ctttctcact caataaaact tttaaactat
     4201 ctacaaatat atcatcaatg tattttccat atatatcctc atttatgtct aaaatatctt
     4261 ttactttact attatatgta tttatttcac catcttttga agtaaataat ataccatctt
     4321 ttgataaatt caatattgta tctaattctt ctatttttaa aaataattcc ttatatttat
     4381 cttttatacc agtatctaaa cttattattt cttctgagta tttaactaga ttagattgta
     4441 tttcctttga atcaatttgt aataaattta ttatttctat aaatgtagaa atatctatat
     4501 atctgtgacc taaatcaaat atatctgaga taaagctagg tactttttct ggaactcctg
     4561 gagttatagc tatttttata tttttatagt tattatcatt catatatgga attggtctaa
     4621 tattagtcac tccaatttta taaaataaac ttatagtttc tagagtagtt tcatcagaat
     4681 catttacgat tagagcattt gtaccctgtg gtaaagatag tagattataa attttatctt
     4741 ccctaaaagt tcttcttacc acaatcactt ttttcttatc tagtatatta tttataattt
     4801 tgtctaatct ttctactgac attactaata tcacatcatc atttataata tcattatcat
     4861 ttatttcatt tatgaagtat ctattaatat ttatagaatc tccaaaaact aatttcaaat
     4921 tattttctaa aaagtctatt aaatcttttc tactatcatt taccagagct actgattttt
     4981 tcatttattt ctccttatat aataaatagt ttccaacaaa atagttgtat aaagcaatgt
     5041 aattttgata atttaaattt atcaaataaa aaagaaagtg tctataatga ctttataaaa
     5101 gttgcattaa gacactttct ttcttataat taaaaatctt gtcaccaaat ccccctaatt
     5161 gttgcttaaa acttaaactg tatgtatatt tttcatttct attcgattaa attataatat
     5221 acacccaaaa aaatttattt taataaattc tattgataat ataattttgt aaatatagaa
     5281 ttatattaaa ataaattata tatgaaaata tatatgtata agtttattct gcgtgagtag
     5341 aatgaatttg tcaagtttaa tttagttgta attgtatctt ttattaaaaa taatataaat
     5401 taaaacatat aaataaaata ttttatacaa tatattgtaa aggaggtaac tttgatgaaa
     5461 gcagaaaaaa caagtatata tccaattatt aaaaataatt taaacaaaat atcaattgtt
     5521 gaatataaaa agggtcaaag atttgtagca tcagataaag acttacaaga agtgttcttt
     5581 ataatagagg gagttgcatt agtggagtgt actactagag gaggaaataa atttcttgtt
     5641 gacattgtac ctgaaaatga atttataggc aaaatcagtt acatatatga acataatttg
     5701 aaatgtgata tatttgcaaa gaccaatata aaattatttc gatttgaaaa aaatgtattt
     5761 gaagagtttt atctcaaacc agattttatt gcactatttc acagaaaatg tacaagaaga
     5821 atatatgaat tatataaaac cagaatggta agagaactat tttcttgcac agaagttatt
     5881 gcctattgta tatcaaataa acaagaaaat aatgtctgca atataaaagc tattcgtaat
     5941 ttcagtgaaa tctattcaat tagtagaaaa agtagatacc attctctgct aaagttaact
     6001 gaaaaggaga tagtaaaaaa aatgggtaat acatatgaaa ttttaaacta tgatgaatta
     6061 cataaattgg catttgaagt aaaagagttt ttggaagatg agtaaaaatt gttacttaat
     6121 caaaataaag tataataatt tctgtaaaaa atagaaatag tctcactgaa aatcaaaaat
     6181 actttagtta ttttcaatga gactgtttag aaatattgta tacttataat gtatagtata
     6241 attattaatc aatttccttt actatattaa tagcccattt tttagataat gctctaggaa
     6301 caccaaatat aaacttaacc acttcttcac tataagtgag taaaacgagt acaaatatag
     6361 gtgcaccttt aaatgcagct ataaaagtta gaggaataga agctaaccac atacatccta
     6421 attctagaaa tagagcatat ttagtatctc ctccacttct taaaatacca attataacca
     6481 atgtattaaa agatttaaga gccattagta ttcccatgat aaagaatatc ttagttatat
     6541 cagaagctaa gctatcagaa acactaaaca tttttaataa taatggtata tttaaaatta
     6601 aaataattcc aagtattaaa ccagctgaaa atacaagtat agaaaacttt ttagcatatt
     6661 caatggctct tttaatatgg tcagctccaa gttcatttcc aagcataata gatgcaccag
     6721 atgcaataca tacagctgtc attatgaaga aattacctgt actggttgct atctggctag
     6781 ctgctatagc agaagtacca gctcttgcat atgcaacaga atacaggaca gttccaattg
     6841 cccatacaga gtcatttaca aatataggaa agctttttga tgaaaaagat tttataaaaa
     6901 ttttatcaat agcttttaga ttttttagac caaactttaa tatatagtct tttttataaa
     6961 ggtaaacata agtaatcatt aaaattaatt cacatattct agcaataaca gtagctagag
     7021 cagcaccttt tacacccaat gcgggaagac cgaagttacc aaatataaat ccataattta
     7081 aaataacatt tgttacaagt gcaaatgcac tacaaatcat acctaactta ggatttctaa
     7141 cacctcttga accagtacta aatacagtac ttacagcaat taatggataa ctaaatactg
     7201 caatactaaa gtaatctata caaagtttta ccacctcaga atcatacgaa aatatgtgta
     7261 taattggctt tggtgttagt aaagcaggaa taacaaatac aaaacttaaa acaattgcca
     7321 atacacaagt aagacctgtg acttttctta tattactcac atccttcttt ccataaaact
     7381 gtgaaataaa aactccagca cctcctgtga taccagataa ggacatatta aaaagaaaga
     7441 aaaattggtt tgcaactcca actgaagcta cagatgtctc ccccaaacta cttatcataa
     7501 ttgtatctat tacatttaca gaagtagaaa ttaagttctg tatgataata ggtatacata
     7561 aagatagaag tattttataa aatcttttat tttcgtcttt taaagaaaat agttcttgca
     7621 aataatcctc tcctttttac ataaattctt cccactcatc atatagagag gcaaggttat
     7681 tttctaaatt aattttttct tggctaactt ctttagcttt atctggatta gagtatactt
     7741 cttcttgaca taaaagtata tctaattttt caatttcaat ctctaacttt tcaatttctt
     7801 tttctatatt ttgtcttttt attctatttt tcttttctat ttctctttgt tctctctctt
     7861 ttcttttttc atcttttatt tgagttctag ttttttcttc tttttcttct ataatactca
     7921 tctcttggat ttgccttttt ttatctatat aataatcata atttcccaga tattcagtaa
     7981 ttccattttc atctaaaact aaaattttat caacaacagt atttagaaag tatctatcat
     8041 gtgatattgt aaatatagtc ccatcataat tagttaatgc ctcttcaagg acttccttag
     8101 aatcaatatc taggtgatta gtaggctcat ctaataacaa taagtttgca tttgatagta
     8161 taagtttaag tatggcaact ctagctcttt ctccaccact caatgtagaa atcttcttaa
     8221 aaacttcttc atctacaaat aaaaatgccc ctaacatatt tctcaaagta gtttgagtaa
     8281 gatgagtatt attttcccaa atctcatcaa ttattgtgtt atctaaatta agagtttttt
     8341 gttcctgatg gaagtaggaa acatgtacat tagttccaaa tttaatattg ccactaagag
     8401 gttgtagttc attagttatt attttaaata atgtagattt gccaacacca tttgcaccta
     8461 taagtgcaac tttttcccct ctatatatat ctaaatccaa gtctttaaaa agtattcttt
     8521 ctccataacc catagataca tctctaattt gaagaacatc attaccactc tggacagaag
     8581 gcgtaaattg tatacgagct tttttcctat aagcttctgg tttatctaaa acgtctacct
     8641 tgtctaatgc tttttcttta cttcttgcac gctttaggtg tttttctcta ccataagctt
     8701 ttagtctttc aattgattct tcttgctttt ttatttcttt ttgttggtct tcaaatttct
     8761 taagctctag ttctttttct attttagata actcaacaaa tttagagtag ttaccattat
     8821 atactttaag ttttttgttg tgaacttcaa aaattctatt tacggattgg tctaagaaat
     8881 atctatcatg agatattaac atgacagttc ctttatattg ttttaaaaat acttctaacc
     8941 attctattgc ttcagagtct aagtggttag taggttcatc aagtaacaat agagttggtt
     9001 tcttaagaag taattttcct aaaagaactc tagttttttc accaccagaa agtatgctta
     9061 ttggcttatc catatcattg tcactaaatc ctaatccttt tagaactcct ttggcttcag
     9121 atttatatcc atatccattt aaatctgaaa ataattctag tttatgtgaa tattcatcca
     9181 tgattttttc tataattgga gagtttgttt ttgtactttc ttcagatatt ttaacctcca
     9241 attctcttaa ataggattcc atttctatca aatttttaaa aacttctagc acttcttcca
     9301 aaatagtatt atccgaataa aaattggtat tttgttctaa atatccgatt tcacaatctt
     9361 tagaagtata aatatctcca ctatcatatc cataaattcc tgaaattatt ttaaataaag
     9421 tagtttttcc agtaccatta acaccgatta taccaatttt atcgccttca tttactgtaa
     9481 aactaatatt ttctaatata gaatctatac caaaactttt atttaagtta ttacatgaca
     9541 aaacaatcat aaatttctac ctccttgaac tatctaaaat tataatgttt tgatataata
     9601 taatcaaggt tatatgtatt agttttgaaa aagaaatatt ttattgtgaa tcaaacaaaa
     9661 aagaggacac ttttaggagg tgaagatgtt gggaaataaa aatatatcaa tggcagttat
     9721 aagaaggctc ccaaaatatc atagatatct tggagactta ttagataggg atatacaaag
     9781 aatatcttct aaagaattga gtgatataat agggtttacc gcttctcaaa taagacaaga
     9841 tttaaacaac tttggtggat ttggacaaca aggatatggt tataatgtag aagctcttca
     9901 tactgagata ggtaaaattc ttgggttgga tcgaccatac aacgcagttc ttgtaggagc
     9961 aggtaactta ggacaagcta tagccaatta tgcaggattt agaaaagctg gattcgagat
    10021 aaaagcttta tttgatgcaa atcctagaat gataggttta aagataagag agtttgaagt
    10081 attagattca gatactttag aagactttat aaaaaacaat aatatagata ttgctgtatt
    10141 atgtatacct aaaaatggag cacaagaagt tattaataga gttgtaaaag ctggaatcaa
    10201 aggtgtatgg aattttgcac ctttagattt agaagttccg aaaggtgtta tagttgaaaa
    10261 tgtaaactta acagaaagtt tatttacctt atcgtattta atgaaagaag gaaagtagac
    10321 aacttaaaca tattaataat gagcagttgt gaagagtatt gagaaagtca gaagatttaa
    10381 tattaaatcc tctggctttt tgaaatatta ggataaaaat ttaaataatg tatggaaata
    10441 aacaatatta aggagagtgg tttttgtgga ggaattatgg aaaaaggcaa ttgaatttca
    10501 tggtcatgaa tgtccagggt tagctatagg atttaaagca gcacttgcag caagaaaata
    10561 tttaggatgt aaacagtcag gagatgagga aattgtatgt atttctgaaa atgatgcttg
    10621 tggaatagat ggtatacaag tcgtgctaag ttgcacaatg ggtaaaggaa atttactact
    10681 taggatgact gggaaatcag tatatcattt ctttaatagg gaaaatggaa aatctataag
    10741 aatatatgta aagtcaaagc catttcaaat ggatagagaa ggatacaaaa agtacttatt
    10801 agaaagtgat tttgaagaaa tatttgagat tacagatact aagttaagac ttcctgaaaa
    10861 agcaagaata tttaaatctc ttaaatgtga agtgtgtggt gaatttgcat ctgagcaata
    10921 tataaggatt gaagaaggaa agaaagtatg tcttgattgc tttaataaat atgaaagatt
    10981 ttatgaggag tattaaaatt ataaattaga ttaaagttaa aagtattaga tatgtactaa
    11041 cagtatttta agatgaatta ggtagcaaca taataaaata tataaataat attacttgaa
    11101 ccatacataa ttaaaaagag gctgattagt atttagaaaa taaaatcagt ctctttatga
    11161 ataacgaatt attattctgg ttgaggtgca tctacaggac aagctcctgc acaagaacca
    11221 cagtctatac aagaaccagc atctataaca tatgcgtcgt ctccagctga tatacagcta
    11281 acagggcatt cagcctcaca agcaccacag cttatacaag cgtcagtaat tttatatgcc
    11341 attttgaaat cctccctttt atatgtattg taattcaata acattataac aaatattatt
    11401 gaatataaca attaaattaa ttctacaaca aaaactttat tatgatattc ttgttttaaa
    11461 gttttacaat aaagtttaat ctgcttatat ttttatttat tttaaagaag tttattttaa
    11521 aaataaaatt aaatttaaaa agttatgtat tgattatatt taaaaatgtt atactctaaa
    11581 aaagtatatt ttattttaaa atttattaat acaaaggaga tttactatga aaaataaatt
    11641 agtttacata tgtattatga gtctattgct atttgtagta ggatgttcta ataatccaaa
    11701 taaggaaagt accattaaaa aagactctaa gaataaccaa agccaagtta aagaagaaaa
    11761 aagagaacct gtgaaaaaag atataactat aagtttcgct ggtgatgtaa ctatggggaa
    11821 ctataaaggt tcatcatatt atggcagttt tgaccatgaa tttaaaagac aaggagaaga
    11881 ttacaattat tttcttaaaa atgtaaaacc tgtatttgaa aaagatgact taacagtggt
    11941 taatttagaa ggtccactta ctacagcgtc aaatgcaaaa gaaaaaaaat ttgcatttaa
    12001 aggaaagcct ggatatgtaa atattttaaa atctggaagt atagaggcag taagtattgc
    12061 aaataatcat tcagaagatt attttgaaga aggaatgaaa gatacacagt ttatattgga
    12121 tgagaataaa ataaattatt ttggattagg gaaagatgct gttgtagatg tcaaaggaat
    12181 taaagtgggt ctattagggt ataatggatt atcaactgaa tataatgaaa aaaatttaaa
    12241 acaaatggaa gatgatatta agtccttaaa aaagaaatcg gatgttgttg tagtgtactt
    12301 tcattggggg atagaattag attattatcc agataaaaaa caaaaagatt ttgctcatta
    12361 tgctatagac aagggagcag atttagttat ggggtctcac ccacatgtta ttcaaggaat
    12421 agaaaagtat aaaaataagt atatagctta tagtctaggg aatttttgct ttggaggaaa
    12481 taagaatcct tctgatacag atagttatat atatcaacaa acctttactt tttcagataa
    12541 taaattaact tcaattaaag agccaaatat aataccaacc tcaataacat ctagtagctc
    12601 cagaaataat tatcaaccaa aagtattgga tggaagtgag aaggatagag ttttaaataa
    12661 acttgaaaaa ataagcaaag atttaaataa ataaaatgta aaaaaaaata aatatatata
    12721 tttattttaa taatttatat tttgtttagg gtatggtaaa tcgtggtatt aaataagtag
    12781 attttaatag ttaaattata gtcaattata ataaagctat ttaatctact tttttaatat
    12841 aaaaatataa gggggaattt gtaatggctt gtaacacttg taaaatgtgc gaaagtgcag
    12901 acaagaaatt agaaagtttt gtagcttcaa aggatgttga aacagcattt catagaacag
    12961 aagatcagaa agttaagtgt ggttttggac ttcagggggt ttgttgtaga ctctgttcaa
    13021 atggaccatg tagagtaact cctaaatcac caagaggtat atgtggagca gatgcagaca
    13081 ctatagtagc aagaaacttt ttaagagcag tagcatcagg agcagcttgt tatttacatg
    13141 tggtagaaaa tacagctaag aatttaaaaa atgttggaat aactaaaggt gttgttaaag
    13201 gagaaaaaac attaaatcaa ttagcagaga tgtttggaat agaaagtgat gaaaagtatg
    13261 ataaatgtat aaaggttgca gacaaagtta tcaacgattt gtataaatca agagatgata
    13321 aaatggaatt agtagaaaaa atagcttatg cacctagagt gaaaaagtgg aaagagttag
    13381 gtataatgcc tggtggtgca aagtcagaag tttttgatgc aatagtaaaa tcatcaacaa
    13441 atttaaatag tgatccagta gacatgctag ttaattgttt gaacttaggt atatcaacag
    13501 gattatatgg tcttacacta actaatcttt taaatgatgt tatgttggga gaaccagtta
    13561 taagaatggc tcctgtagga tttaatgtaa tagacccaga ctacataaat ataatgataa
    13621 caggccatca acattcaaca tttgcaaatt tccaagatag attaaaagat gaagatgtta
    13681 taaaattagc tcaatctgtt ggtgcaaaag gctttaagct agtaggatgt acatgtgttg
    13741 gtcaagattt acaattaaga ggagaacatt atcaagaagt atttgctgga catgcaggta
    13801 acaattttac aagtgaggct gttttatcaa ctggagcaat agatatagtt ctttcagaat
    13861 ttaactgtac tataccaggg cttgaaccaa tagcagataa atataaagta aaaatggttt
    13921 gtttagatga tgttgctaaa aaagcgaatg cagaatacat aggtttagac agaagtaaat
    13981 tagatgaact tagtaacact ttaatagaaa aagcattgga gtcttataag gagagaagag
    14041 gaagtattga gatagatata ccaaaagacc atggatttga acaatcttta actggtgtaa
    14101 gtgaaaagaa cctgaaagcg tttttgggag attcatggaa acctcttatt aatttaattg
    14161 cagaaggaaa aattaaaggt gttgcagctg ttgtaggatg ttcaaatatg acagcaggag
    14221 gacatgatgt aaatactgtt gaacttacta aagaattgat taaaaaagat ataatagtac
    14281 tatctgcagg atgttctact ggaggattag aaaatgttgg tcttatgtca cctggagcag
    14341 aagaattagc aggagaaaac ttaaaagaag tatgtaagac tttaggaata ccaccagtat
    14401 taaactttgg accatgtcta gctataggaa gactagaaat agtagcaaca gaattagcag
    14461 ctgaacttgg aattgaccta ccacaattac cattagtgct ttcagctcca caatggttgg
    14521 aagaacaagc attagctgat ggagcgtttg gtcttgccct tggcttacct cttcatttag
    14581 ctttacctcc atttataaca ggaggaaaat tggtgacaga agtattaaca gaaaaattaa
    14641 aagacttaac aggaggacat gttatagtga atccagaccc taaatcttca gctaatcaat
    14701 tagaagaaat aataatagat agaagaaaaa atttagggtt aaaggatgtg gaatgcaatg
    14761 catagaattt ttataaataa agatttatgt acaggatgta agagctgtgt actagcatgt
    14821 atgctaaaac acaataaaga ttatgacatg tacactcttg atttagaaaa tatagataat
    14881 gatagtagag ggcatataga attagatagt aaacataata atccagttcc aattctttgt
    14941 agacactgtg atgaacctga atgtgtatta gcatgtatga gtggtgcaat gcataaagat
    15001 agtgaaagtg gaatcgtttc atatgatgaa gagaaatgtg gttcatgtta tatgtgtgta
    15061 atgtcttgtc catatggatt attaaagcca gatgatagaa gcaagcaaaa catacttaaa
    15121 tgtgatttat gtaaagatga ggagtatcct agatgtgtag ctaattgccc aagtggagca
    15181 attgaattac agaaggagga aaatgatgaa ttatgtagtg ttaggggcta gtgcagctgg
    15241 tataaatgca gtaaaaacat taagagaatt agataaagat tcaaatatag tagtaatttc
    15301 taaagatgaa aacgtatatt caagatgtat gttgcatcac gttatatctg aacacagaac
    15361 tttaaaacaa ataaattttg ttgatgaaga ttttatggag caaaataatg taaaatggat
    15421 tgcaggtaag actgttaaag gtatagacat aaataaaaag gtagttcaaa cagaagacat
    15481 tactgtaaat tatgataaac ttttaatagc tacaggtgca tcttctgcta ttcctcctat
    15541 aaaaaattta agagatggaa attttgtata ttctgtgaga aacatagatg atatttataa
    15601 aataaaagaa aaagcacaaa attctaaaaa tgtagttata ataggtgctg gtttagttgg
    15661 tatagatgca ttagtgggat tatttaaata tgagcagtta aatatttctg tagcttttat
    15721 ggaaaagtac attttagata gacaattgga tgaatataca gcatcagtgt atgaaaataa
    15781 gttcaaagaa aaaggtgtaa aattttaccc aagtgcatca atacaagaaa ttgtattgga
    15841 taacagtaaa aatgttacag gtgttgcatt ttcaaatggt gaagttttag atgcagacat
    15901 ggtaattgta gctacagggg ttaaaccaaa tgctgacttt ttagatggga caggaataga
    15961 atatgataga ggtataataa tagatgatat gtgccaaaca acacaaaaag acatatatgc
    16021 agcaggagat gttgtaggta aaaatgctat ttggcctctg gcagtaaagc aaggaattgt
    16081 agctgcctat aatatggttg gaaaagataa gaagatagaa gatgaatttg cttttaaaaa
    16141 tagtatgaat ttcatggata taccaactat ttctatagga atgaatacac ctgtagatga
    16201 tagctataaa gtattaacca gacatggaat taatgattat aagaagtttg tttttaaaga
    16261 taatgtaatc tacggagcag ttatacaagg agatatttca tatgtaggtg tattaactta
    16321 tttaataaaa aataaagttg aaatatatga tttagaaaat aggatatttg atgtatgtta
    16381 tgcagacttt tttaatataa aagaaaatgg agaattttgt tatagtgtat agtatatacg
    16441 tggagtaagg tttataaata cagtaaatca ataaatagag ttattctaaa ataagaaaaa
    16501 tgacaaatct tatttagaaa tagctctatt ttttgtaatt aaatttaaat tatattcaat
    16561 ttgtaaaata ttattttgaa caattataca tatactctct agttagaggt aaatcatttt
    16621 ttataccttt agtgaaaagt atttgatgta tatctataat accagtgttg aaagcagcag
    16681 cacaagaaca aaggtataaa tcccacattc ttataaactt atcatcaaac atttctttaa
    16741 tctttggaag attctcagag aaactttttt gccagtatag taaggttttt acataatgta
    16801 atcttaaact ttctacgtct atagtatgat atttgtaatc actacaaata tgtatcattt
    16861 ctcttaggct aggaataact cctcctggga aaatatattt ttttatccaa gggtctccat
    16921 aagattcctg caatccactt atatagtgaa gtaaaaacaa tccaccatct tttaaaacag
    16981 catttacgtt tttaatgaaa agttcatagt tttctctacc aacatgttca agcattccaa
    17041 cgcttacaac tctatcaaac ataaggtctg atttttttaa ttctctatag tccataagtc
    17101 ttacctcaag atagtcttga aggttctctt ctttaattct tgcgttaaat tccttaaatt
    17161 gctcttcgct aagtgtaatt ccaacgccgt gaattttata tttttttgca gcttctatta
    17221 gtaaaaatcc ccaaccacaa ccaatatcga gaaggctcat accttcctct aaatttagtt
    17281 tagttaatat gtggtgaact ttattgactt gagcatcata aagagtatca ttatcatttc
    17341 taaagtatgc acaagaataa ctaagtgtct tatctaacca taagctataa aaatcgttgc
    17401 caatgtcata gtgggcatgt atttctttct tctgatgtct cattgaattt gatgagtgca
    17461 atagatgttt tacagctgaa ttatcaatat tgaatttgtc tatttgtttt aaaagtagat
    17521 ttaaagttaa gaataaatct ccctcaactt caatgtctct atttatgtag gcttctccaa
    17581 gtgctaaaga tgtacttttt aatagttcag acttgctaat atcattattt actataactt
    17641 tgaactgagg ggagccctct ccaattagaa attcttcctc atctttaagc ctaactagaa
    17701 atggtgtttc accaaatttc tctaaatagt cttttaaaaa attttttgta atattcatat
    17761 taccagctcc ttctaatgta aatccagtaa gtttccctgc ttaaaatata ttatagtcct
    17821 ttttttaaaa aagtaaaatt ttttcaataa attttttatt taaaggaaat ttatttttta
    17881 taaagaaatt tcatgttgta gtatattttt tgcttataaa ttttggtact ggatataata
    17941 ttaaactgtt aatgatataa taaatttaat ctataaagta aataaaattt tgtaataaat
    18001 agttaaagct attttataag gagatatatg atggaagtat atgctttggt tgggtcaagt
    18061 ggcacaggga agagctttaa ggctttagag ttcgcatatg aaaatgatat agaatatata
    18121 atcgatgatg ggattttaat atacaaaaac aaggttttag ctgggatatc ggcaaaacaa
    18181 gcaaatacta ctatagaagc agttaaaagg gctatttttt ataatctgga gcatagacaa
    18241 gaagttagag aagcaattaa aaaagaaaat ataagaaaaa ttcttattat tgggacatca
    18301 aaaaaaatgg ttaatcaaat tgtagagaga ttgtcaattg gaagggtctg taagtttata
    18361 aatatacaag atataagtac taaatatgaa atagaaatag caaaacaagc tagaaaagaa
    18421 ggaaatcatg taattcctgt tccagccgtt gagataaaaa gtatggcaag tggtctcagt
    18481 ataaattctt tgaagagact ttttagaaaa ggaaataata ggaatatggt agttttagaa
    18541 aaaacaatta taagaccaac ttttagttat ataggaaagt tttatattag tgctgatgta
    18601 attaaacaga ttattgaata tgaagtatac aactttggaa gcattgatag aataaataaa
    18661 atcaatatag agaattgcaa taattttatg aatatttttg taagtataaa tatcaatgat
    18721 ttagaaacta taaaaaaaat tgaagacata caacagacaa taaaaaagtc tattgagaaa
    18781 atgactttag ttaatgtaca aaaaatagat attaacattc ataaaattaa aaaaataaac
    18841 ttcctccaat gttaaaaaat aaactgagaa aatgatatac taatttgcaa taaagtgtgc
    18901 tataattaaa ctgtaaattt aggaaatggt tttttctaaa tggagtatac ataattataa
    18961 cgtatttata agggggcttt caaatgtcag gaaaagatgt aaatgtcttc gagatggcgc
    19021 aatctcaagt aaaaaatgca tgtgataaat taggtatgga accagcagtt tatgaattat
    19081 taaaagaacc tatgagagtt atagaggttt caattccagt aaaaatggat gatggttcta
    19141 taaaaacttt taaaggattt agatcacaac ataatgatgc agtagggcca acaaaaggtg
    19201 gaataagatt ccatcaaaat gtttcaagag acgaagtaaa agctttatct atatggatga
    19261 ctttcaagtg ttctgtaaca ggtataccat atggtggagg taaaggtgga ataatagtag
    19321 atccatcaac tttatctcaa ggtgaattag aaagattaag tagaggatac atagatggaa
    19381 tatataaatt aataggtgaa aaagttgacg ttcctgcacc agacgtaaat actaatggtc
    19441 aaataatgtc ttggatggtt gatgagtaca ataaattaac tggacaaagt tctataggtg
    19501 ttataactgg taagccagtt gaatttggtg gttctttagg aagaacagct gctactggat
    19561 ttggtgttgc tgttactgca agagaagctg ctgctaaatt aggaatagac atgaaaaaag
    19621 ctaaaatagc tgttcaaggt ataggaaacg ttggttctta tacagttctt aactgtgaaa
    19681 aacttggtgg tactgttgta gctatggctg aatggtgtaa atcagaaggt tcttatgcta
    19741 tatacaatga aaatggttta gatggtcaag ctatgttaga ttatatgaaa gaacatggta
    19801 acttattaaa cttcccagga gctaagagaa tatctttaga agagttctgg gcttcagatg
    19861 ttgatatagt aataccagct gcattagaaa actctataac taaagaagtt gctgaatcta
    19921 taaaagctaa attagtttgt gaggctgcta atggaccaac tactccagag gctgacgaag
    19981 tatttgctga aagaggaata gttcttactc cagatatatt aactaatgct ggtggagtta
    20041 cagtttctta cttcgagtgg gtacaaaact tatatggata ctactggtca gaagaagaag
    20101 tagagcaaaa agaagaaata gctatggtta aagcatttga atctatttgg aaaatcaaag
    20161 aagagtacaa tgttacaatg agagaagctg cttacatgca ttcaattaag aaagttgctg
    20221 aagctatgaa attaagagga tggtactaag atttactctt aataaataat aggatataat
    20281 tatttaagat ataaaggggc tgtacatagt acagccctta aattatgtgg cttacaatga
    20341 agtaagctat ttttgtttta acttataaat aacttgataa attatgtaat ttagaatgca
    20401 taaataagat gggtgaaaat taatggttga tttaattttg aatgtatttt ttaccaccac
    20461 atgattacaa attagaggag ttagattgga tttttggaca tgaacttatg tattttaaaa
    20521 gcaaagacct tcatttaaag tatctagtat tatttttaaa aatagtatat tggtttaatc
    20581 catttatata tataatggaa aaatatatgg atttggattg tgaactttat tgtgatgaga
    20641 gagttttaaa aaattgtact gtacaacaaa agcaagaata tgctctaact attgtaaatt
    20701 caatgaaaag aggtataaat ttttcaaata aatttgtaac tggattaaat aaacaatctg
    20761 atattagaaa aagagtaatg aacatgttca gtgaaaaaca taaaaatggt atcttaatgg
    20821 ctttgattat aagcttgttt tcttctatta cattttttaa attcgattca actagtctta
    20881 ttgaagagaa tgatttctta gagaggtcaa gatatgcgtt tgaaggaagg caagaagaca
    20941 tgtttgtaac aatagattct acatatgcag atgcacctat aaaacttaga aaagagcatg
    21001 aagaaaactg tagagtcaat ggaatagtac caaaagattc agattcaata gaaattattc
    21061 ctagatttta taaagattgt atggatttaa aaaaataagt tagacttaat attgatgttg
    21121 atattatgaa ttttttatat ttaagcaatt gaaaatttta ttatagattc aattttatat
    21181 gtgtttaagt atgaaattta tagatagttg aaataattaa atatgattga tgcataggaa
    21241 tcattactaa gctataatta attatagggg agtttaaata aattatgagt tacacaatta
    21301 taaaatgata aaaaatagtt atctgttaca aaaaatgtat ctttttaatt aataaagata
    21361 atatgaaaac ttaaaaggca aataaactta aacataaaat actcttaaat aagataggag
    21421 aaaaatgtat gtctaacttt attctaaatg ggatttttag aacagttata gtaagtagtc
    21481 taagcattct tctgatattg attttcaaga aaaatgtatt taaaagattt agcaaaaaat
    21541 ttaattatta tatatggatg atagttgtta ttaaattatt tttaccattt acatattata
    21601 cttttacaat aaatatcttg agaagtaaaa aacacattaa tattaataac ataaatttag
    21661 agggatttaa taatgtttca actataagta ataatattat attgtacact tggattataa
    21721 ctgtagtagt gtatttaatt tacactatat ttaaatatat aaaacttaaa aatttaataa
    21781 atgacttatc ttatgatgtt gatgatagag agattgtaaa tctttataaa aatatattag
    21841 aagaatttaa tataacaaaa gatataaaac tcaaatactc ttatgaagtt gaaacaccag
    21901 cattttttaa ttcatgtgta cttttgccac cacatgagta taaattgaaa gagttagact
    21961 ggatttttag acatgagctt atgcatttta agagtaggga cctttattta aaatatgtaa
    22021 tcctgttttt aaaaacagta tattggttta atccatttat atatataatg gataaacata
    22081 tagatttaga ttgtgaactt tattgtgatg aaagagtttt aaaaaatcga aattcagagg
    22141 aaaaaaagga ttatgcttta acaattctaa atgcaatgag aaaaggttca aatacttcaa
    22201 ataaatttat agctggactc cataagcaat ctgatattaa aaagagagtt atgaatatgt
    22261 ttaatgaaaa gtacaagaat ggtatattaa tggcattaac tttatgttta ttatcttcta
    22321 ttactttttt gaaggttaat tcaacgagta ttgttaatct taatgtatta cctaaaggat
    22381 ttgaagactt acgaactgtt agcagtatac gtttttcagt tataggattt acatatgcag
    22441 atgctcctaa aaatatgagg gaagaatata aaataaattg tgaatcatta ggtttgatac
    22501 ctaaagactc tgataaaata gaagttggtg tagaatatta tgacagtcta ttgaatgatt
    22561 aaataaatat tattttaaca gatattaatt ataaagtttt gaatagtaat ttgttgagaa
    22621 ataaatatat ccttataaaa atagatactt atataccaag aataaaaaat agttaagata
    22681 gtacacaagg atatacttat aaagaactta aataagtgaa ataaattatt aattataagg
    22741 tttataattt taaaaaatta gataaattca ggtgtaaaat actataaaac acaattcgga
    22801 aaagtgtatg ttgaacttca tttaaaatag aattttcaaa gttgttatag taagtagttt
    22861 aagcattctt cttatattaa tctttgagaa taatgtactt aaaaaagtta gtaaaaaatt
    22921 taactattgt atattaatga tagttgctat tgatatatta tctaaaaaaa ttaagtatga
    22981 aaagattact gctaggacaa tcttttcata cttaattttt taatatttat attctccaaa
    23041 attttcatct ttttaactta gaattattag aatttgagaa tcactattag gaaataggtt
    23101 ttgatttatg cagggttaaa aatttcacag aatatctaac tttatactaa atccatcaaa
    23161 aaatatacgc catataaaag cgcataaaaa aacacataaa acaatattta tgcgcacttt
    23221 aacctcgaat ggtacaaaat tctttacttt tacagaccca tattatataa ttaagtaaat
    23281 aatgtatatt taggtgacat attggataag ctaatttatt tattttatta atataatgat
    23341 actataaaag ttatataaag tggtttacaa aaaagttaca gatagagctt ttattaatgt
    23401 attgtgtatt agtattttat ccttaggtac acttcaccta caaaatagaa tcataagata
    23461 attatgggaa aattttagga ggtttatcgt gaagaaaaag atattaatac cagtatttgc
    23521 atcagttatg gcattatcag taagttcaat tgtaaatgca gatgaagtaa atgattcatc
    23581 tcaaaataag gatgataaaa caaatactga attaaattta ggtgaatata aagaagtaaa
    23641 atataaagtt gcaaaaataa aagatggagt agctataaag ataagagaag aaggtcaagt
    23701 tcaaaacata gcttattctg gagatgaatt tacggtttta ggaactcaag gagaatgggt
    23761 taaggttaaa gttgaagatg gcgaaggttg gttagctact agatatgtag atatatctga
    23821 aggtgtagga tatactaacg ctgataaagt aaacttaaga aaagataaga gtgaaagttc
    23881 agaagtaatt gaagagctgg aaaaaggaag ttctttattg gttttggagg ataatggaga
    23941 ttggttaaaa gttaaagatg gggaaacaga aggatatgta aaatcatctt atatatctga
    24001 taaagctcct gtaatagaag agccacaagt aaacccaagt attgaccaaa atgtagattc
    24061 tacaaataat aatcaacaaa acaatgcaaa tactaatcaa caaaataata attcaaatgt
    24121 gccaacagct aactctaatg cagttcaagc tgtacttaac ttggcatatt ctaagcaagg
    24181 ttgtccatat gtttggggag cagaaggacc aaacactttt gactgttctg gatttacaca
    24241 atatgtgtat agaaatgcag taggaaagaa tataccaaga acatctaaag cacaatctaa
    24301 atatggacaa actgtaagta aagcaaattt acaaccagga gacttagtat tcttcacaac
    24361 taatggttct ggttctgtta gtcatgttgg aatatatgta ggtgggggta atatgataca
    24421 ttcaccatca acaggaaaaa cagttagtgt aactagtata aattcatcat actatacagc
    24481 aagatttgtt acagcaaaaa gaatattata atcggtttgt aatatgacaa attgataaat
    24541 acataaatat atataaacgc tctatattat atttaattta atcatggaaa caaaaaatga
    24601 ttatatttta aatgtactat agagcgtttt tgtatcactt taaatctaat caagattcaa
    24661 gttaatatga ctattaaata ggagcctttt catatgatga ttatttgcgc aatcccttga
    24721 caggataggg taaaggtggt aagctactaa cagtacaaaa atacttttaa ttttagttca
    24781 gagaggctaa aaaaggagga cgaaagatgt taaaatcaag aaatttaatc caaccagaag
    24841 acttttcaat agaagaaata gatgaaatat tagaattagc tcaaaaaata attgacaatc
    24901 cgtcaaaata ttctcgtata tgtgaaggaa aactattagc tacattattt tatgaaccat
    24961 caacaagaac aagattaagc tttgaatctg ctatgaacag attaggagga cgtgtagtag
    25021 gcttttcaga gccaaattca tcctctgcat caaaaggtga aactctagga gatacaatga
    25081 ggatagtatc tggttatgtt gatataatag ctatgagaca tcctcaatca ggtgcagctt
    25141 cagaagcagc tagatatacg gaagtgccat ttataaatgc aggagacggc aaaaaccagc
    25201 atccaactca aacacttaca gacttactta ctataaaatc attaaaagga actttagaaa
    25261 gccatacaat aggattatgt ggtgatttaa aatatggaag aactgtgcat tctttggtta
    25321 aggctatggc aagatataaa aatacaaagt ttgtatttat agcgcctgaa gagttaaaaa
    25381 tgccagatta tataaaagaa gctataaagg gacatgcata ttatgaaact aataatttgg
    25441 atgatgttat aggaagcttg gatgttttat atatgactag agttcaacaa gaaaggtttg
    25501 aagataaatc agagtatgaa agacttaaaa actattatat tttaaataaa gctaaattag
    25561 aaaaggcatc taaagatatg ttagtaatgc atccattacc aagagtaaat gaaatagaca
    25621 tagatgtaga tagtgatgat agagctgtat attttaaaca agctaaatat ggaatgtatg
    25681 ttagaatggc tttaataata aaacttttag gtataaatga agactaatag ttatcatata
    25741 atagtaaaac aaaggagatt ttgggatgta taaaattctg gaaaatatat atataggcga
    25801 agatatgtat agaatgaaag taaaaggtaa ttttgaaggt aaaatgggac aattctatat
    25861 gttaagagca tgggatactt atccagtact ttcaagacct ataagtatac atgatataga
    25921 tgaagaaggg ataacttttc tttataaagt agtaggagaa ggaactcaaa ttttgagtaa
    25981 cctaaaggta aatgataata taaaattaga aggcccttat ggtaatggat atgctaaggt
    26041 tgatggaaag gtagctctag taggaggagg aatcggtgta gctccactat atttggttgc
    26101 taaaaatata aaaaattgtg atgcatatct tgggtttaga gaagatgtta tattagaaga
    26161 tgaatataag caagtatgta ataaagtcta tacaacagta ggaaatactt ttgtaacaga
    26221 tataattgat gttgagaaat atgattatat attaacatgt ggtccaactc caatgatgga
    26281 aaaattagtt aagatggtag aaggtactaa aacgagaatt atggtatctc ttgaaaacca
    26341 tatggcttgt ggagtaggtg cctgtttagt atgtacgtgt aaaactaatg gaggaaacaa
    26401 aaagacttgt aaagatggtc cagtgttctg gggagaggac gtgattttta atgggtaatt
    26461 taagtgtgaa atttggaagt gtagaattta aaaatccagt aataatggcg tctggaactt
    26521 ttggatttgg aaaagaatat aatgagattt atgatataca aaaacttggt ggtataagta
    26581 gcaagggtct tactttaaat aaaaaacctg gtaacaatgg tatgagagtt catgagacac
    26641 catccggtat gatgaatagt gttgggcttg aaaaccctgg agttcaagga tttatagact
    26701 atgaattacc tttttttagt aaactagact tagtcagaat agctaatgtt ggaggtggga
    26761 cattagaaga ctacttatta ggggttcaaa tgttaaatga caagcctata gacattattg
    26821 aattaaatat atcttgccca aatgtcaaag ctggtggaat ggcttttggt ataaaaaatg
    26881 aagtggcaag agaagttgtt cgtgaggtaa gaaacataac gaaactgcct ttagtaataa
    26941 aattatcacc caatgcagaa gatattgtag gaatggcaaa agtttgtgaa gaagaaggtg
    27001 ctgatggagt ttcattagtt aatactttta aagcaatggc aattgatata aaaaatagaa
    27061 gaccagtatt tgaaaatgta tatgctggat tatctggacc agctataaaa cctatagctt
    27121 tgagaatggt acatgaagtg tgtaaaaatg taaatatacc tgtaatggga atgggcggaa
    27181 taactaaagc cacagatgct atagagttta taatggcagg agctacttgt atccaagttg
    27241 gaactgcaaa ttttatgaat cctagaatag gtattgaaat aatagatggg attaatgaat
    27301 ttatggatag agaaggaata aaaagtttag acgaaataag aggaattata taaggtagaa
    27361 taaatagtat tttaaagata aaaaatatag attaaataaa caataatttt ataacattaa
    27421 catggaggaa aaataatatg agtaaagtag atgtagtgga tatattaaag aaaagtgatg
    27481 cattgttgga gggacatttt ttattatctt caggaaaaca cagtaataga tacgtacaat
    27541 gtgcaaaagt attaagattt ccagaatatg ctgctgaggt attaagtaca gttgttgaac
    27601 aaataaaaga cttagatata gacttagtag taggaccagc tatgggtgga gtaatagttt
    27661 cttatgagtt aggaagacaa ttaggaaaag aagctgtatt tactgagaga aaagacaata
    27721 caatggagtt aagaagagga tttgaagtta aaaaaggagc aaagataata attgctgaag
    27781 acgttgtaac tactggtaaa tcaactatgg agacaaaaag agtattagaa gccttaggtg
    27841 gagaagttgt aggtgttgca tgtatagcag atagaactaa tcatgatata ggtatgccta
    27901 tatatagtgc tataaaactt gatattcaag tttatgaatc tgatgagtgt cctttatgta
    27961 aggaaggaaa attaccagtt gttaagcctg gaagtagaga gttcaaggaa ttagggatgt
    28021 aataaaaact taattattta tagtgttact taaaaaatgt aaatttttta gagtaaaaat
    28081 ttgtaatata acatataact taaaaataaa aagcagttag ccttataaat gaaggttagc
    28141 tgtttttttt gttaaaaata tatttacaaa ataagagata gtagcagtac aatataagta
    28201 taaggtaaaa gtattatata ttagggaggg gtaagtatga aaaatcaatt aaatgaaaat
    28261 cataaaaagg ttgtattttt tggagtaggt gctgtaggag ctacttttgc agaacaattt
    28321 tttaactcta aatatgattt taaaattctt tgtgataatg aaagaaagaa aagatattta
    28381 gaagaaggat ttataataaa tggaaaaagg tatgatttta attatgtaac taaagatgag
    28441 tataaacaag aggctgattt tataattata ggtctaaaat ataataattt aaaagaaaat
    28501 ataaaagaat tagatggatt agttggaaaa aatacagtta taatgtctct gctaaatgga
    28561 gttgatagcg aagagataat aggagaaaga tttggaattg gaaaaatggt atattcatat
    28621 gttaccaata tagatgcaaa gaaaatcaat aataatatta tacacactac taatgggata
    28681 attgtatttg gtaacaaaga taatagtgaa gatagaaaaa ctaatataat aactgaagtc
    28741 tttgatgatg taaatataga atatatttta tcaaaagata ttcaacgaga tatgtggtgg
    28801 aagtacatgg ttaatattgg tgtaaatcaa acttcagcta tacttggtgc accttatgga
    28861 gtttttcaga gttctgagca tttaagagaa ttagcaaaat ctgcaatgag ggaagttgtt
    28921 gctatagcac aagcaaaaga catatctctt acagaagatg atgttgaaaa ttcattacat
    28981 agaatactag aacattcaaa agaaggaaga acatcaatgc ttcaagacgt ggaagctcat
    29041 agacctacag aagtagatat gttttctaag aatatctgca aacttggaaa aaaatatagc
    29101 atacctactc ctataaatca gactttattt tatatgataa aagtaattga aagtagattt
    29161 taaagggtat ttgtaagaaa tattgataat tattgatttt aaattttatt aatgttatat
    29221 actatttaag ttactaaata tttttaactc ttcaatgaaa atagttgctg cttttgacaa
    29281 agttatatct tttggaaata tatatgagaa atgtctgttg tagctgcttc caagctctat
    29341 gacagacagc tcattgttta aaacaggtaa gcttgtcact agattagaaa ctattgtaat
    29401 tcctaaatta tttctaacag attctttgac agcataatta cttccaaaaa ccatcatact
    29461 ttttggaata atttctttaa cacttaaaaa catatctaaa tagtctctgg ttccagaacc
    29521 atcctccctg actatccatt tttggtcttg aagtttatca aaactaaaat cttttaataa
    29581 atgactattg tatggaagtg ctaaaaccat cttatcttca aaaaaatatt cttgaataaa
    29641 tgaaggggaa gagcaagtgc cttcaataag accaatgtct aaaatataat ccttaacatg
    29701 agaagatacg attgatgtat tttttataaa aacttctaca tatatgtcag gatacttttg
    29761 agaaaataat gctaaaaaat ttggtagaat atattctcca atagttaaac ttgcaccaat
    29821 ctttaagtga ccagttatag catctgaaac ttggcttaca tcatgataag taatatttaa
    29881 aagatttaat atttctttag ctcttttgta caatatttgt ccactttctg ttataaaaat
    29941 ggttttttgt ttaatagacc tgtttataag cacaacttta aaataattct ctagattttt
    30001 tatgtgttta cttacacttg gttgtgatag atttaagtat tccccagctt tagtaaagtt
    30061 tttgtattca acaacagcta caaatgtttt taattcttca aacaattttt tgtctccttc
    30121 acctaatgaa attttacatt ttttatatac tttctacaga taattatact atatataata
    30181 accattaaaa acattaatca ttacgatatt atatatttat tttacttatg gttattacaa
    30241 ttatatacta ttgctatgaa gaaaactata ttaattataa ggtaggtata ttatgttaaa
    30301 gaaaaaaagc agtttgacat taaaaactat aagggaaata ttaccaggac tgtttatatc
    30361 agtgttagta ggttatatta gtatatttat ttctatttta attcctaagg taggggcagc
    30421 atcaatttct atatttttag gtatgtttgt aggaaaccta tttttaaatc aaaaggtatt
    30481 tcaaaaaggt tataaatttt cagagacaga tttgttatca tattcaatag tactattagg
    30541 ggcaacactc agtgtatcta ccttaataga cttaaaagtt tcaggaattt tatttataat
    30601 attgcaaatg acaataacaa taatagctgc actttatata ggtaaaaaac taggatttga
    30661 agaaaacttt agatttctaa tggcaagtgg aaatgcagta tgtggttctt ctgccatagg
    30721 agcaatagtt ccagtggtta atgcgacaga taaagaaaag ggaattgcag taactattgt
    30781 aaatgttact ggtatattcc ttatgttttt acttccaata atatctcaat ttttatatag
    30841 ccatgaactt gtaaaaactt cagcaatgat aggtggaact ttgcaatcaa ttggtcaggt
    30901 tgtagcaagt ggagcaattg ttggagaaaa tgtaaaagat ttggctacta tttttaagat
    30961 agtaagagta atatttctag ttgttgttgt attagtattt ggacatatga aaaatcagtc
    31021 taatagggaa atccttgaag aagaaaagga tgagataaaa aagaaaaaag taactatacc
    31081 atggtatgta attggattct ttataacttg tgcacttttt tcaatgaaca ttataccaaa
    31141 agaagtatct gtgttgtgca aagaaataag taacaaacta gaaataatag ctttagcagc
    31201 aattggatta aaagttaatg taaaagattt agtgaaacaa gggaaagaag tttctttata
    31261 tggtttattt gtaggtacag tacaagttgt atcagctgta gttttaataa aaatatttat
    31321 atagcacaaa aatagtattt gtaatattaa
//
LOCUS       DAMKPG010000039        30940 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMKPG010000039 DAMKPG010000000
VERSION     DAMKPG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.042"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..713)
                     /gene="yqeC"
                     /locus_tag="PC621_001911"
     CDS             complement(<1..713)
                     /gene="yqeC"
                     /locus_tag="PC621_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF4109070.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCV"
     gene            complement(896..2173)
                     /locus_tag="PC621_001912"
     CDS             complement(896..2173)
                     /locus_tag="PC621_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4109071.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            complement(2312..4618)
                     /locus_tag="PC621_001913"
     CDS             complement(2312..4618)
                     /locus_tag="PC621_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF4109072.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            complement(4611..5405)
                     /locus_tag="PC621_001914"
     CDS             complement(4611..5405)
                     /locus_tag="PC621_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF4109073.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            complement(5511..6869)
                     /locus_tag="PC621_001915"
     CDS             complement(5511..6869)
                     /locus_tag="PC621_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF4109074.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            complement(8216..9307)
                     /locus_tag="PC621_001916"
     CDS             complement(8216..9307)
                     /locus_tag="PC621_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109075.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(9537..10622)
                     /locus_tag="PC621_001917"
     CDS             complement(9537..10622)
                     /locus_tag="PC621_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF4109076.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(10658..11971)
                     /locus_tag="PC621_001918"
     CDS             complement(10658..11971)
                     /locus_tag="PC621_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF4109077.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(11972..14263)
                     /locus_tag="PC621_001919"
     CDS             complement(11972..14263)
                     /locus_tag="PC621_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF4109078.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(14253..15047)
                     /locus_tag="PC621_001920"
     CDS             complement(14253..15047)
                     /locus_tag="PC621_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF4109079.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(15086..15532)
                     /locus_tag="PC621_001921"
     CDS             complement(15086..15532)
                     /locus_tag="PC621_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF4109080.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(15604..17010)
                     /locus_tag="PC621_001922"
     CDS             complement(15604..17010)
                     /locus_tag="PC621_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4109081.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(17028..18398)
                     /gene="hydA"
                     /locus_tag="PC621_001923"
     CDS             complement(17028..18398)
                     /gene="hydA"
                     /locus_tag="PC621_001923"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF4109082.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(18531..19718)
                     /locus_tag="PC621_001924"
     CDS             complement(18531..19718)
                     /locus_tag="PC621_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDF4109083.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(19845..21059)
                     /gene="dpaL"
                     /locus_tag="PC621_001925"
     CDS             complement(19845..21059)
                     /gene="dpaL"
                     /locus_tag="PC621_001925"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF4109084.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(21422..22093)
                     /locus_tag="PC621_001926"
     CDS             complement(21422..22093)
                     /locus_tag="PC621_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4109085.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(22176..24392)
                     /locus_tag="PC621_001927"
     CDS             complement(22176..24392)
                     /locus_tag="PC621_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF4109086.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(24395..24865)
                     /locus_tag="PC621_001928"
     CDS             complement(24395..24865)
                     /locus_tag="PC621_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF4109087.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(24927..26321)
                     /locus_tag="PC621_001929"
     CDS             complement(24927..26321)
                     /locus_tag="PC621_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4109088.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(26394..27755)
                     /locus_tag="PC621_001930"
     CDS             complement(26394..27755)
                     /locus_tag="PC621_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF4109089.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(28053..29375)
                     /locus_tag="PC621_001931"
     CDS             complement(28053..29375)
                     /locus_tag="PC621_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF4109090.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(29731..30771)
                     /locus_tag="PC621_001932"
     CDS             complement(29731..30771)
                     /locus_tag="PC621_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109091.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         9332 a         6123 c         3381 g        12104 t
ORIGIN      
        1 acacaaaatt tttgttttac gcttccataa aatatctcta tttcactttc atattcttta
       61 atattttcta ttaattttat agctaaattt ttatattttt cattttcaac tttattaata
      121 aataatactc tttttccaga ataattttta aataatccat ttggatttaa tactatattt
      181 tttaagtttt caatacttac tgtagaacta ctattaatac tacttgtatc taaatttgct
      241 atttttttaa atatttctac tctatgtata ttctcttcat taatattcat tccaaatgat
      301 gttatatcta atatccctat ggtttttgta gttttatgat atactactgg ttctttagca
      361 ttccaacctt taagtttttt tctctttgag ccatctccct ctattaaaac taaatcaaat
      421 tggtctacaa tttcatccaa aatagaggaa tcaatactta ctaatttatt ttcattattt
      481 atataactgc cacagactgt tattccatga caaatagatg gaataacagt tccatctatt
      541 gtaataatta tattgtcata atcgtttggt acatatattt tagttgttgt tgtaagaaga
      601 actttcaatt tatctctata aaaatttgca aaatagttta taaacgaagt ttttcctcct
      661 gctccaacta cagttattat ttcattcttt tctattatat ttgatagttc cacactttca
      721 cctcttataa ccagttttct aatctcttaa ttattatagt tagacctttt tagtaaaagt
      781 gatttttgaa attccataaa ctcgaaagta aatagattct gttgtaaaca acaaggcgga
      841 tccaattcgc cgctaagctg taagcttacg ctatcagttt atcttttaaa tataattaag
      901 atattcttac tctctgtggt tgctttaata tttgaggatt attctgtaaa ttagataaat
      961 taactacttg tgattttttt agtatattaa gactcgcatt tacatctgca tttataatga
     1021 ttccaacact ggatttgtac tgtccacgtg ttattctttc ccctttaaag tcatattttt
     1081 taacattgtc cacattgata tcaggtagaa aatcatcagc aaaaaaatca gacttactag
     1141 tatagctttc ttcttgccta ataaaattga tattttttat tttacataga tattctaatt
     1201 ttccttttat ctctccaaat gggattctta taaaatttct atatattttt ctctccatat
     1261 tatcactttt ttctaactta gcattatatc ctacaattaa atttccaata ttatttttta
     1321 cacaataatc aattatataa aaacaggttt tatttacata gtctttaact tgattatttc
     1381 tcttattcca aactttaaat tgttttcttg ttattgcagt tatattcttt tttaatttta
     1441 aggattctat tttatcattc tgtttgtttg cccactgatt cacagatttt agcctttttc
     1501 catctatgat aaaactatca cctaaattgg taacacatgt acataaattt tctaaaccca
     1561 agtctatagc tagtgcattt ttttgatttg aaacttcctc tgatttcggt atttcataac
     1621 aatactgaat ttcaaagaac ctagctttat gtttaggtat tattcttatt tctttaattt
     1681 ttttctctag tatattttgt ggaactttta tacttacttt cccatatagt tttttaaatg
     1741 ctggagacat aggtacattt aaaatcccat cttctttgat tcttatttga cctattatta
     1801 tgctaaaaaa tccttcttta tctaaatatt taggtgattt tatccctcta aagtcatatt
     1861 tcccttcttt ggctaattta attaacccaa aaaatgacct aaacgaactg tctacttctt
     1921 ttaatatttg ttgcgctata ttactattga gaagcttata gttctcattt tctttgcata
     1981 catgataatt agcttcatag ttaagatgtt ctccttgatt gaaataatgc tgtcttacat
     2041 tatatgtacc aacattatat aagttcttag ctaagaagca catctctcgt agagcttgat
     2101 attctttttt agttaagttt tttaattgtt gtctctgagt tccatagtgt atatttttta
     2161 ttctgtttga catgaactca cctcctttag aatatactct actagacatt atatacggta
     2221 tttttacaaa acaccatata taatgtccat ttaattactt tattttacaa aataatttaa
     2281 atttacttgc ttttatacac cttattattt attataattg tttattatta tttttcatac
     2341 ccatataaac tttttcaaaa gtcataggaa gctctcttaa acgaactcct gtagcattaa
     2401 atattgcatt tgctatagct ggcgctggtg tatgtataac tatttctcct aatgatttag
     2461 caccataagg acctcctggg tcgaaattct cagcaaaatc taccaatata tttccaactt
     2521 ctttttttgt aggaacttta tactgtagga aactatctgt taataatctc ccatctttat
     2581 caaatatagg gtcttcatac attgcaaacc ctatacccat catcacacca ccttctactt
     2641 gtattctagc taaagttgga tttataactg ttccacaatc aggtacacat gcaaaattta
     2701 tgaccttata ttcgcctgta tttttatcta attctatctc tgcaaatcct gctataaatg
     2761 gttttatttt tttcaacatg ccaaaagatt ctgttgcttc caaaactact ggtacatcat
     2821 cactagcaaa tcctattcct ccaacagatt ctctacccaa ttcctctaat agaacaaact
     2881 tgctattatc gtttttaaca aaaaccctgt cttttcccaa ctctaaatct tcttttttaa
     2941 atcctagctt tgactctgca actgaaagta atatatcttt taatttattt gctgtcttta
     3001 ttgttgcatt tcctgtaacc gttgttgtac aagaagcaaa tgctcctgta tcatagggac
     3061 ataaatctga atcacctgta taaatagaaa ttctatctac agtagtattt aaagcttccg
     3121 cagcaatttg agcttgaatt gtatcagccc cttgacctaa gtcagcaaga ccagataata
     3181 gtttatatgt gccatcttca tccattctta tagtgactgt tgccatatct acacgcggta
     3241 taccagaacc atgatttgct actgccatac ctactgctct tactttgttg tttcctatat
     3301 ctttacatgg atatttatca tcccaattga ttaatttctt gcccttttct atacattgtc
     3361 tcagctcaca acttaatatt tcgtcttcag ctgcttttcc attggtatac ctatctatta
     3421 tatttttaat tctaaattca agtgggtcca ttccaatctt atgtgccaat tcatcaatag
     3481 cactatctat tgcaaatatt ccctgtgtag caccataccc tctaagtgct cctgctggta
     3541 acctattagt atatactgtt ctagcattaa atctaacagc atcaatatca ttatatattg
     3601 gtaaggtatt gtgtccagat tcggaagaaa cagagacacc attcccacca taagcacctg
     3661 tattgtttaa tgactttagc tctattgctt ttaaatttcc ttctttatct gcaccaactt
     3721 taacatcaaa aaacatttca tgtctactat tagaacctac aaaagtttca cttcttgtaa
     3781 atacaaattt tgctggtcta ccagttttca tagtaacaaa tgcagtaaat atatctgttg
     3841 ctgctatatt ctttcctcca aatccaccac ctattctagg tttaattact cttatttttg
     3901 aagatggtat acccaatact tttctaatta ttctctttac attgtgtggt gattgtaatg
     3961 atgaaactac aacaattcta tcatttatat ctttatatga atatgaccta tgagtttcca
     4021 tcatgcaatg cgattgactt tgggttttgt atgaagattc agccacaaaa tcactttctt
     4081 taagcttagc ttcaacatct ccaaattcaa aaaattcttt tgatactata ttattttttg
     4141 catccaatcc aaaagggtat ggctctacta tgtctttttc tggatgtata acaacttcat
     4201 ttccttctgc ttctcttgca tcaaacacag gctttaaaac ctcataatct acctttatca
     4261 tttttaaggc tttaagtgct gttttctcat cttcagcagc cactaaagct acttcatctc
     4321 caataaatct aactgtatct tcaagtaaaa ctctatcata aggtgatgct tctggttctg
     4381 tttgacctgg cacacaatat ctgacctgtg gtacatcttt atacgtataa atacccacaa
     4441 cccctggaat tttttcggct tttgatgtat ctatattctt tatcttagca tgagcatgtg
     4501 gacttctaag tagttttaat gtcaaaacat ctttatgcat aatgaggtct tcagtatata
     4561 gaggttttcc tgtgacaatt gctgtactat ctatctttct tatcccctta ttaatcatta
     4621 tacattccct cctcaatagc attatacatc cttttaacta atgcacttgc catatccttt
     4681 ctgtactctt tactagctct catattactt ccataagtaa gttccttact agcaacaata
     4741 gatgccttat ctatatcttt ttctttattt aatatattac tcgcttcaag tgctaatcta
     4801 gctttttgag gtcttgcccc aattgctatt ttgtaagtat cattttcttt tagcattgct
     4861 gaatttaata cagaaaaatc tcctgtgcat tttcttattg agtcaaaaac agctatcgca
     4921 tctttttttg gtagtattac ttcgaccaat atatccctac ttaattcact atccaaaaac
     4981 tcacttaact ctagtattcc tttattgtat aatctcacct tcgctccaac aaccaaaagc
     5041 gctggtatta agtcagaaaa gccatacttt gaaaatacac ttgcaccaat tctagcccct
     5101 tgtctaaatt gaacaccaac tatattagaa acagcgcttg aaagtattcc actacagtaa
     5161 ttttttatag ttttatcaat ttctaaactt ctaagtgaag tatctgcacc tatcaaaata
     5221 tttttttcat cctcttttat ataatttaga tttatatctt ttaaatctat cgctgttcct
     5281 atatttttat tacttaattt tataaatcca catccaccta aaataacatt atttttttta
     5341 gaaattaaaa ttttatatgc ttcttctaaa ctctgtggaa ttgaatattc ctttatagta
     5401 accatcttat cccccatcat taacttatta aaacacaaaa gtttttaata aatcttttat
     5461 ttatatttat aattgtagta tatttaattc acatatatta aacctttaca ttataaactt
     5521 ttatttttat cctcaacctt atcactttta tttccaggaa attttaccat atataaagct
     5581 tgaattatta tggctaccat tgctcctgaa actacacttg aatctgcaag taatgtctct
     5641 ataaattttg gaaatttttc aagaacttga ggagctacca tgaatccaat accagaagcc
     5701 atagaaagac ctaatatcat gctattttct tgattaaatc catccatact aacaagtctt
     5761 aaaccagatg ttgtaagagt tgaaaatata acgagtgttg ctcctccaac cacacaggga
     5821 ggaattgttc ttattaaatt agctaataaa ggcgatattc ctgccaaaaa taatccaatt
     5881 cctccaatag ctagtacaaa tttacttaca acctttgtat tggaaacgat tgcagaattt
     5941 tgtccaaaca tccccattgg agttgcagaa aataatgcac ctattgtaga gagtactcca
     6001 tttcctaaaa cagcagaaga aagttcttca tctgttactt gtctattaag ccctcctacc
     6061 gcagaaagtg tacaagcacc cattatatca gctatctcaa ctaaatatat tattgaaaac
     6121 attacaacaa cttcaagtct tatatctagc ccaaaagcaa gtggttttgg aagcgcaacc
     6181 aaagtaaact cctgaattga agaaaaattt actataccca aaattaatga taaaatgtat
     6241 ccaaaaatta tgctaactaa tattgatgca tctttaaata atccttttcc atacttattt
     6301 aatcctaata tcataagaga tacagatatt ccaactataa aattaattgg attcccaaaa
     6361 gtctcattgc ccataccccc agctaagttt tttatagcta ttggaaaaag tccaagaccc
     6421 atacatgcaa ctattgttcc tgatacagct ggagtaaata taaatcgtat ttttttaaga
     6481 ccaaaaccaa ttataattat tactatacca gctattattt gagagccaaa taatactggt
     6541 agtccatact tacttgcaac agatacccca gctcctataa acatagctgt agctcccatt
     6601 ataactggta ttcctcctcc taatttaaaa cctttaaaca aaggtattgg atataattgt
     6661 aataaagttg ataatcctgc aaaaactaat gcggcttgaa tcaataatgt tgacatctta
     6721 tgattaagtc cagatgcatt tgcaatcaat atcgctggta aacaactacc tattaccatt
     6781 gccattaagt gttgtaaccc taaaggtatt gcttccttta gtggtggtat tccgtctaat
     6841 tcaaattttg atgtactttt tgctttcata ataaaaactc ccgttacata aaacttattg
     6901 aattatataa atacttaaaa atctaatata ttaaattaat gatgttcata aatatcaact
     6961 atactccaac atatatttaa gtttttagtc ttaaaaaaca gatgcaataa atttaattat
     7021 taacaaatac acgtattctt taattttgta tacattatat accatgcttt acaaatataa
     7081 gagtattata gctatgaatt tttaattttt tatagtaaat ttaattcaat ttaaatcttt
     7141 caatcttaat cattataagt tataagtttt cataagtatt tacatgtttc aagttttaaa
     7201 catttaccta taaacgctac tttatagatg tcacttcaaa tctagtgacc tcatactttc
     7261 atatgagcgt agactatttc ttcatcttca gcattacctg ctaagagcaa cccgtttcca
     7321 ctatcaatcg cttatagtgt actctctctc gagatagtcg tttgaccttc ctatttctag
     7381 gcttggcgac caaatgtcca tttttaagta cttaggctct caccatatac catcctcact
     7441 attgtttctg gttttcacca cattcataaa agtttctttc tatcttttat tgtagttgca
     7501 aggctttagg atttactggt tttaaagttg tgtcctacat agatttctct atgtacgggg
     7561 ccaaaagtac aatttattta acatactttg ctttaccccc tgtaaataat attatttact
     7621 ctttcactgt ttttgttatt aaattaatta aatatcaatt aatctaagca taaaataatt
     7681 atttaattta aactcaaaat atttatgaat attttataat ataattctca taagacagaa
     7741 aaacttaata agctatgtat ctcagttgta ccaatgtact ctcctttact atatttgtat
     7801 ctgcaacata aaatatacaa gtttctatct atttatagat atatatatct ataagcaaaa
     7861 tagtaatatt atttttatag taaaaatttt attcatatta ctatttataa tttttataga
     7921 gcaattttag tgccaatatt tgcatacatt tgtatagtat aaaattttta tgttctcatt
     7981 gataaaattt tacttatttc aagcgtttta ttcgatttta tcatctaaat tttaggtttt
     8041 atttttataa attaattgta tttacataaa aaaaaagacc taaaaacaaa tttttaacgg
     8101 tcttttctca attttttatt ttcatttcta aaatttataa taaatagtaa gaacactata
     8161 aattctttta tataaaattc ttagtttgtg gatgataaat tagtgaatta aaatactact
     8221 ttactgattt tagtttgaca atatcactcc aatttttact agtggctaac tctactaaat
     8281 ttaagtctct tcttatttca gctatatcct cttttataga attagtgtca ccttttatat
     8341 atgctatgtt atgttctata ttatcatgag tagctttatt tatttcagaa ttatgtaata
     8401 aacttcctaa aatattattc tgttctatct gtatctcttt cattgattta tcttcacatt
     8461 ctagactatc aatcttaatt tcaattccat ataatttttc atttgctatg gatacctcgt
     8521 ccttaattcc attcatatct tcattaatgt tatttatttt attttgcata aagtttactt
     8581 ctgatttaac ttcggaaaca tcttctttca ttacagttat ttcttgtctt acttctgaca
     8641 tgtctttttt cattacattt atctcttgtt ttacctctga tgtatcttct ttcattacag
     8701 tcatttcttg tcttacttca gacatatctt ctttcattat atttatctct tgtcttactt
     8761 cggacatatc ttcttttaat acatttatct cctgttttac ttccgacata tcttctttca
     8821 ttacagtcat ttcttgtctt acttcggata tatcctcttt cattatattt atctcttgtc
     8881 ttacctctga catatcttct ttcattacag tcatttcctg ttttacttcc gacatatctt
     8941 ctttcattac agtcatttcc tgttttactt ccgacatatc ttctttcatt acaatcattt
     9001 cctgttttac ttccgacata tcttctttta atacatttat ttcctgtttt acttccgaca
     9061 tatcttcttt cattacagtc atttcttgtt ttacttccga catatctcct tttaatacat
     9121 ttatctcctg ttttacttcc gacatatctt ctttcattac agtcatttct tgttttactt
     9181 ctgatatatc ttctttcatt acagtcattt cttgttttac ttccgacata tcatttttta
     9241 tgctaactac atcttcattt atagtatata ataactctaa aatcttctct tgcacttgca
     9301 tcaccatccc tattaattat tataacattt tacacaaatg ctatacaatg ttaacatata
     9361 tatcagaatt attaaaattt taattaaaca aaattgattc actttattac ttcaaaacta
     9421 attaatatct aaaataacta caaataaaca aaaataaggt caacaacaaa aaaggcttat
     9481 ctctttgaga tagcctttct tatttttatt taaaactttg ttttttataa aataatctat
     9541 acattcagct ctgaacatga aatttttctt ccaagtgctt ttggtttaca aactgatata
     9601 caaagtccac aatatctaca ctcttcttca ttcaattcca tcttatcatt caattttctc
     9661 gcttgatatg cacaaactct ctcgcacatc ttacatttag tacatttttc ttcatcataa
     9721 gtaaactcaa agctaagtct ttcattaaca tctcccatat gattatgtac tataccacaa
     9781 gcattttcta tagtattata tcctaatcta ttaagattag attttaaatc atcatgtatt
     9841 ttagatatta cttgtgcgcc ttttaaaatt actactgaac atataccaac aaagtcagca
     9901 cctgccataa tcatttctaa ggcatcatct ccacttacac atccacctaa gccaattatt
     9961 ggtatattaa cttgtttttt tatttcatat attctctgta aagttattgg cttaatagtt
    10021 tctccactta aatatcctat tccatttcca cctaataaag gttgccctgt ttctatgtct
    10081 attctaaatg ctggtcctac agagtcacaa gcagttattg catctgctcc tgcttcttct
    10141 aattttttag caaaatcgcc tatctcatct atcataggag gaagtttaac tattacaggt
    10201 atatttactc tttcctttgc atctttaagc attggtatca aatctctata gtcataagat
    10261 actaattcaa taaagtcagc tcctgcattt gcaactttct ctactaattc gctactttct
    10321 tcaattgtat gacctactga agctatacag acagtcccat ctcttttcat ttgtggtatt
    10381 tcaaaatcta tccattgctc tggctcaaaa tctgtccatt tttcattatt taaaagagca
    10441 ttagctgtat ttctaaccat atgtggtgct ggatttatag ctctttcttt tcttatagtc
    10501 ttagtcacaa ctgcccctgc tcctgcatca cttaatatct tgcatccagc tgcactatat
    10561 gttaatggtc ctgaacctat tataagtggt gattttaatt ctttacctaa aaaattatac
    10621 attatctgct tccccttttc atatatattt acattcgtta aaatttccaa aattcacaag
    10681 caacttcttt catttcttta acaatagctt ttttatctaa atcaactaac tgtttttctt
    10741 ttaaaatatg tctaccatct atgtatacat ttgatataaa ttctttttta ccctgaacta
    10801 ctatctggtc aaatatatta tcaagagtca caggagtttt aaattcatct tccataacta
    10861 ttatatcagc cttattacca actgctattt taccagaatt ttgaagtcct aatacataag
    10921 caccatgctc tgttgccatc ctaaatacta aatttgctgg cattactgat gcatcttctt
    10981 caactgattt atgcattaaa aatgcacctc tcataacttc aaaaaaatca tttatatatc
    11041 catcagtacc cagtgccact tttatgcctt ttttaatcat ttttggtact ggagaaaaac
    11101 ctccacctac ttcacagttg ctaagaggca tatgaaccac ttttacacct ttttctttta
    11161 atatatctat ttcttcatca tttactttta cacactgaga tgctaataca gtttcatcca
    11221 atatatctaa atcattatag tagtcaactg cttttttgcc aaaatgtttt tcagcatagt
    11281 ttggctcata tatactctca cataaatgaa attgcattgg tgcatttaat ttttttgcat
    11341 cttctttagc tttcttaata aatctatcag aacatgtgaa gcttgtatgt gtacacatta
    11401 ttccatttat taatttacta ttttctctac tccactttat taaattagaa ttttcatcta
    11461 aacatctaag tccattttca aagcttattc tttcacaact ttctaaggat aaaatcgctt
    11521 tcatgcctat ttcttctagt atttttcctt gctcaattaa agttccttct tctgtatttg
    11581 gagcttctag ggtatcacaa aaagctacca caccagattc tataagttct attgctgatg
    11641 ctttagttgt tgccttcact tctttcaatc caattctatt ttctataaat ggccaccaat
    11701 agtcatttaa aaaaccttca aagtcagtaa aatgtacatt tgccggtatt cccctagaaa
    11761 gaactccata ttgatgcata tgaccattga cgaatcctgg cattattata gcatctgatt
    11821 tatctattat ttcttcaata tttttatatt ttatttcaac ttcttcattt ggcaatatat
    11881 cttttataat gtcattttct actacagcag cagaatttgt atatatttca tcagcagaag
    11941 atattaagta tttactttta atagcaatca tttatttacc tccctgattt aattttttca
    12001 tggccatata aactttctca gaagttatag gaagttctct tatacgaact cctgtggcat
    12061 tgtatattgc atttgctatt gctggtgcag gtgtatgaat aactatttct cctaaagatt
    12121 tagctccata tggacctgtt ggctcataat tatctacaaa atcaacaatt atgtttccta
    12181 tatctttttt tgttggaact ttgtattgta atagattaga agtttgaagc tttccatcag
    12241 aaccatgtct agggtcttca tacattgcaa gccctatacc ttgagttaca ccaccttcaa
    12301 cttgtattct agcaagtgca ggattcataa ctgtaccaca atctgtagaa caagcataat
    12361 ttattacttt aaactcccct gttgttttat ctaactctat ttcagcaaat cctgctaaga
    12421 atggtttagc actctcttgt ataccataag attcagatga atataaaact tcttgatttc
    12481 caccaccaac agattctttt cctaattcac ttaacaaaac aaacttctct acatcttcac
    12541 tataacaaac tctgtcttca tatagtatta aatcatctat tggtaaatcg agtttcttct
    12601 gtgctacttt taatatcttt tgttttaaac tttcagcaga ttttattgta gcatttccag
    12661 ttacataagt agtacaagaa gcgtatgcac cagtatcata tggacacata tctgtatctc
    12721 ctgtataaac ggaaattcta tcaatagatg tattcaatgc ttttgcagct atttgtgcaa
    12781 gtattgtgtc agaaccagtt cctaagtctg aagacccaga aaacaactta taagttccat
    12841 cttcatccat cctcatattt actattgcca tgtctatgtt ggctatacca gaaccatgag
    12901 ttcccacaga cattccaaca gccctcacct tattgtttcc aatatcccta acagggtact
    12961 tttcatccca tccaataagt tttttacctc taattataca ttcatcaagt gcacaacttc
    13021 ttattggaaa attcattatt ccgccttcag tttgtttttt tataatattc tttaacttta
    13081 tttctgttgg gtccatatta agtttatgcg ctaattcatc aattgcacaa tcaagtgcaa
    13141 atgtaccttg tgttgctcca tatcctctta atgctcctcc tggaactaaa ttagtatata
    13201 cagttctccc atcaaatttt attgcaggta cattattata tgttggaagt acattatgac
    13261 ctgattcaga gcaaactgaa ggtccattat caccataagc gcctgtatta ttcaatgctt
    13321 tcatatctat agcttttatg tttccttctt tatctgcccc aatttttata tcaaaaaaca
    13381 tctgatgtct agtattagtc attgcaaaag tttcttctct agtataaatt agttttgctg
    13441 gtctttttgt catccaagtt acaaatgatg catatatctc tgttacagct atgttttttc
    13501 caccaaaacc tccacctatt cttggtttta ttactctaac ttttgaaaga ggtattccca
    13561 gtgctttaga aacctgtctt ctcatatgat aagctgattg gtttgcagaa gttactacta
    13621 atctgccatt cgtatctata tacgtataag ctctatgagt ttccatcata caatgggctt
    13681 gtgattgagt catataagaa tgttctacta ttacatcact ttctttaaac tctttttcta
    13741 catccccttt acccattttt tctctcgata caatattttt tgtagcatcc aatccaaaat
    13801 caaatggaca aaacaaatca tcttcttcat ggattaaaat tttatttcct tccgattctc
    13861 ttgcatccaa tataggagtc attacttcat attcaacttt tataagtttc atagctttaa
    13921 tagcagccct ttcatcttca gcggctatta tagcaacttc atctcctaca tatctaacta
    13981 cgtcttcaag tattaattgg tcatgaggtg atgcttctgg atatgactct ccaaccattg
    14041 aatatctata atttggaaca tctttgtaag tgaatatgtt tactacacct tttaccttta
    14101 atgcattttt tgtatctatg ttttttatct tcgcaaaagc atgcggactt ctaagaagct
    14161 taattgttaa aacatcttta tgaaaaatta aatcttctgt atatattggt tttccagtaa
    14221 caatggcttc actatctatt tttcttacct ctttatttac catcacactc acctccaata
    14281 ctattataca ttctcactac taaggctttt gccatatctt ttctgtattc tttacttcct
    14341 cttatattac ttccaaatga aagttcttca cttgatactt caccagcttt ttctatatca
    14401 ttttctaaac ttaatatttc acttgcttta taggcaattt ttgctcttct tggtctagcg
    14461 cctatcgcaa ttttataaat acttccttct tttaacattg ctccatttaa tactgcaaaa
    14521 tcacctgtac attttcttat agaatcaaat acacctatag catctttttt aggtaatata
    14581 acttctatta atatatcttt ttcaagctca ctttctaaaa aatcagctaa gttcataacc
    14641 ccttttttat ataacttaac ctttgcatct accactagta atgaaggtat taaatctgaa
    14701 aatccatact tagcaaatac gctagctcct actctggctc cacttctaaa ttgaactcct
    14761 actatattag aaacagcgtt tgaaattacc ccattacagt aatttttaat aaccttattt
    14821 aattctaaag ttcttaaact tgtgtctgct ccaattaata tgctatcatc agtttcattt
    14881 atataatcta aagctaagtc ttttaaatct attgctgagc caatattttt actacctagt
    14941 tttaaaaatc cacatcctcc aagtattatg ttgttttttc ttgatattag cagttcataa
    15001 gcttcatcta aactttttgg aactacatat tcctttatag ttatcatact ctctccccat
    15061 atcaacttaa ttttcactta aaagtctaag tgtttaaata tttatgtatt gctcttaatt
    15121 gacctttata tcctgtacat ctacaaaggt taccatttac ataatctata acttcttctt
    15181 ctgttggatt ttgaagctct cttttcattg ccaaaaccat cattataaat ccaggagcac
    15241 aaaatccaca ctggtcagcc ccttcgtctg caagaaaatc tccaaacttc tttgcttcat
    15301 ccaagacccc ctcaagtgtt gtaatatgct gaccatctac tctagcagta agataattac
    15361 atgaaagcac ggccttgtca tttaccatca ctgtacataa cccacatgag cctgtatcac
    15421 accctttttt aacacttaca tatccatttt ctcttaatgt atctgctaaa aaatcatctg
    15481 gtcttatact taattctttt tctttattat ttatggtaca tttgataatc aaataaatca
    15541 cctcaaaact catattttta agctaaagtt ttactatact tttctatgaa taaattacat
    15601 ttattaataa aggctattcc atttatgacc ttccattcta ttttttatag ttcctcttac
    15661 acaaagtctc tcagctgtat cttgagcatc ttttaatgct ttctcttcat tagctgtcaa
    15721 tatttttgaa tcttccatta ttatatttcc atctactata acactctcta tatttttcat
    15781 actagaagaa tatactaatg tagataccgg attatgcata ggtatcgctt ttggtgaaag
    15841 cattggatta aatattagta agtctgcttt tttacctatc tccaatgaac ctatctcatc
    15901 ttccattcca atagctcttg ctccatctat agttgccaac tcaagaactt tttctgccga
    15961 cattgctaat gggtcacact tattaacttt atgttgaagt gctgttaatt tcataagttc
    16021 aagcatatct tgagagttat tactagcggc tccatctact cctaaactaa ctgttattcc
    16081 ttttttaagc atttctggca ctggtgcaac acctgaagat aagtacatat tacttgctgt
    16141 attatgagac actttcatat cgtatttttt agtaagttcc atatcttttt cagttagata
    16201 cacacaatgt accataagca catttggtcc aagtattcct aatttttcta aaacatctat
    16261 gtcatattgc ccatgcaatt cttttgctgc ttctctatca aaaggagtct ctgatatatg
    16321 tactgtaaat aaagcatcat catattcttt taccactctc catagcattt caagcatttc
    16381 ttgagaattt gaccatattg ctgcaggagc tactcctatt ttgattcttc cattttctgt
    16441 attatggtgt ttttcaaaca atcttcttac atctttttca accgtttcta catcttgcat
    16501 tattccagga tgaactccaa attgagctcc tgtattcata catcctcttc caagtattcc
    16561 tcttattcct aattctttat aagcatctat tattccatca caaagacctg gcttactatg
    16621 aggatacatg tagtcaacca tggtcgttat accacttcta agtccttcaa tacatccaag
    16681 cattgcagca tcataagtat cttttggctc taaatagtta gctgctggaa atgtcatagt
    16741 ttctaaccag tctttaagta ccatatcatc tcccaatcct tttaataaag tttggaataa
    16801 atgattatgt gtatttataa aacctgggaa taaaacttta cctttagcat ctataatttt
    16861 tttaacatca gtatatttac tttctatctc tttagaattt ccaatatcag caatcttgtt
    16921 atcttttact actaatgccc catcaaaaat aacttctctc tctttattca cagttactat
    16981 tatggcattc ttaataagta tatccatcat tcttatttcc ccccgcatta tttagtaatt
    17041 tttcttttaa tatattgacc tgttggatgt tctttaacat tacaactttg tactacatga
    17101 tttcctctca aaaatacatc ttcaacttta catttaacct taaatccttc atatggagta
    17161 taatctacat tttgtttttg agttttataa ctaattgttt cttctttatt gatatttaaa
    17221 atgactatat ctgcatcact tcctacagct atctcacctt ttttaggata cattccaaat
    17281 atttttgcag cattcgtaga agtaacttcc acaaacttat ttacacttat tctattttct
    17341 aagaccccat atgtatataa aagtgctaac ctatgttcaa caccaggtgc tccatttggt
    17401 atcttactaa aatcatcaat tcccaagtct ttttgcccct taaaattaaa tgaacagtgg
    17461 tctgtgccaa ctgtttgaat atctccacta gacaatcctt cccaaaggta atttatgtct
    17521 tctttttttc ttaaaggagg agacattaca tacttagcac cctcaaattt attcttatta
    17581 tataaattgt cttctaataa tagatactgt gggcatgttt ctactatcat atctatattt
    17641 ttttctctag ccatttttac agtttcaagt gactccctac aacttaagtg gactacatag
    17701 gattttgaat tagataattt agttatatca gcaagtcttg aaacagactc tttctcaact
    17761 atgttaggtc ttgtaagtgg atgatatttt ggctctaaat taccattttg tatattttct
    17821 tctatcaata catctagtaa atccccattt tcacaatgaa atccaactat acaacctaaa
    17881 tcccttgctt tctttaaaac cctgtatatt gttccatcat ctactttcat gccatcatat
    17941 gccatataca tcttaaaaga tactatccca tccgaaataa gtttttccat atgattaaat
    18001 gtatctttat ttaaacatgt aattgtcata tgaaaaccat aatcacaata acaatttcca
    18061 cttgcttttt tatgatatgc ttctacacct tgcagtaggt tttccccctc attggcttct
    18121 gcaaaatcta gtattgtagt agttcctcca gctatagctg ctcttgttcc tgtttcaaaa
    18181 ttatctgcag ttgtaataga acctgcattc atatcaaaat gagtatgtgt gtctattcca
    18241 ccaggaatta caattttacc tgtagcatca ataaccttat caccatttat ttctaagtta
    18301 tttcctattt caactatctt ttcatcttct atccttaaat cacttatata agtcttatca
    18361 gaggtaatta tagttccacc tcttagtatt gttcccatat atacctccta acttttaagc
    18421 tttaaagtat tttctaagta tcgtaaaact ttatttaaaa ttgtattttc taaaggtgct
    18481 atattcagca cctctagact atatttaaat aatttattgt gaactaaata ttattttccg
    18541 tatacagatt ttaatattcc atagtaacct tctgttccta taaataactg ttcttgctct
    18601 atatactcat ctattgtgtg agcaagattt tccttagaag gaccaaatcc tatagttttt
    18661 attccagctt cacctgcata gtgacttcca ttcgtacaga atgagtattg agttatttct
    18721 gaatctattc cagcttcttt taatccctta tatgctgctt gaacaaattc atcttcttcg
    18781 tcatataacc aacctgggaa gaatctttca ctttctatag tatttcctgt atagcaatca
    18841 gctttttcaa ccgcataaga aactttcgca tttaattctg ggtcttcttt catcatttca
    18901 tctaataaag cttgtattgg agctaatacg ccttctcttg tttctccaac taataatctt
    18961 ctatcaaaag tagctttgca ataatctggc actactgatg cccctggata tggagaagat
    19021 tttatatctg ttaaaactaa tattccatca cctaaaactg gatgagttgg aggtactaat
    19081 tgttgtatct tttggattac attagccatt ttataaactg catttacacc tttttctgga
    19141 ttagcagagt gagctggttt accaaaagtt tctactacta tttctcctct acctctctga
    19201 ccaatcttta agtttaactc tgaagactca cctattacaa catagtcagg ttgaacagcc
    19261 ttacttatct ctctagccga aacaccttca aatatttctt catgaacaac acctgctaca
    19321 tatagttctc cagcaaaatc tcgattagtg tctttagcaa aatacgctac agcagacatc
    19381 attgcagtat attgaccttt catgtcagaa gttcctctac catatatctt tccatcaact
    19441 atttttcctt caaaaggagg aacactccat ttacttgcat ctggaactgg aactgtatct
    19501 atatgtccat caaataatat tttcttacct ggcttgttac cttttataca tcctactata
    19561 tttccatatc tatctcttga ccaactatca aaacctaatt tttcaaaaga ctcttctatt
    19621 gcttttacaa caccttcttc atttccagaa gaactaggat tttgaattaa tttttggcat
    19681 atttcagtta cttcttgttt tctttgttcg tttaacattt cattatcccc ctagattttt
    19741 tataatgtat gtattttata tacttatata ttttctaaat tttagaactt atatatcttc
    19801 taaatatgta aaccaatatc taataattgc attttagtaa ttccttatat acttggatat
    19861 tctccatccc aaactatact tctatatttg tcagggtcag tatctccttc tgtgcttatt
    19921 aaaagtattc ttgaattttc atctaatttt aaatcttttc ttaaatctgc taaatcttct
    19981 ctttccatag ctgctactaa aagaccagtt gtaactgctc cagattctcc agaaattacc
    20041 ttttcatcac catttaaagg attacctaat gttctcatac ctttagcaga aacccagtct
    20101 ggtgctgaaa caaatgctgc agcatgattt tttaatactt caaatcctat agtatttggt
    20161 tctccacaag caagaccagc cattatagtt ggcatatctc ctccaacaaa tcttggtttt
    20221 ccatctcctg cttctgctga tttatagtag caatcagctt catcagcttc tactactact
    20281 gttattgggc attcatcgta tattgaagca acatatcctt gaactgctcc agcaagtgaa
    20341 ccaacccctg cttgcacaaa tacatgtgtt ggtctatcta ctccatattc ttttaattgc
    20401 tctatcgctt cttgagccat agttccatat ccttgcatta tccatgctgg aatttcttca
    20461 tatccatccc atgctgtatc ttgaaccatt actccatgat catcagcttc tgcataatca
    20521 ttagctaatc ttactgcatc atcatagttt aagtctgtta tactagcatc tgctccttct
    20581 cctttaattt tatttaatct aaactcagaa gaaccttttg gcattaatac aacagatttt
    20641 tgacctaatt tattagctgt ccaagcaact cctctaccgt gatttccatc tgtagctgta
    20701 aagaaagtta tttctcctaa tttttctttt atttctttcg aagttaattt gtcataccct
    20761 aactcagata tatctgtatc taatctttga gctaagtatc tacccataga ataagaacca
    20821 cctaatactt taaatgcatt taatccaaat ctataagatt catcttttac atatactcct
    20881 gcaacaccta atttttttgc taaaccttct aggtttgcta atggagtttc tttatattga
    20941 ggaaagcttt tgtgaaaatt tctaacctta gttatttctt cttcatttaa aaatttgata
    21001 caattttcct tatcaccctt tggcaaatta tttactttcc atttaatttc ttttaacatc
    21061 taaaatacct ccaaattatt gttaaatttt tatactttaa tatataagca attaacatgc
    21121 caacactaaa tatatactag atatttttct atgactcttt agtatagaaa ttgcaatgct
    21181 tttattattc tttcttttct atctttagtt catacaatgt atgtagaatt taataactaa
    21241 aaaacctttt ctatcttctc actttttaat tctcattttt aaaatttaaa aataaatctc
    21301 aaaacacttt gtttctatat tttttaatat aaaatttata aaatctaaat taaaacataa
    21361 aatagtgatg caataatcta ttacatcact atttgtatat atttaataat ataataaatc
    21421 tttagtggtc tctaagaggt gatttttcat cttttttaac atctaattct tcttttacga
    21481 cattccattt ttcaagacca aacttagaaa ccataactgc ctctgcagca ccttttccaa
    21541 gtctttttac tgtgtccata cctgtctcat cctcttgggc accttttgct tttccatctt
    21601 tactccatat tgtaccacct atatagcatc cacctaaacc tccagatact aacatttcat
    21661 gcattaggaa gaaatttatt ataggaagtt ttgtcaattc ttggccacca tatctagttc
    21721 caccagtagt tatagctgca ccaaccttat tttgaagttt tcctggatga actaaataca
    21781 ttggacgaag tctattaaat actgctgtaa gttgagcagt tatattcata tcataaactg
    21841 gagaacctac tataattcca tctgcctcca atattttttg ctctaattct tggatatcat
    21901 cttttataat acacatagtt tcttttctta tacatgcatc acaatgtaca caaggactta
    21961 tttttttccc tctaactgac caatactcag tttctatacc aggtatagtt tctgcttctt
    22021 tcaaagcttc taacactgca tattctgttg ctcctttacg tggacttcca caaataccta
    22081 atatctttgc cataataata tctcctcacc aaataaatct atttttatta tctatttaca
    22141 aatacaataa attgttgttt gtgctttaat ttctactatt ttaattttat tggcaatgaa
    22201 tgtatttcta catcagttgc attatatact gcatttgcaa cagctggcgc aactggtaca
    22261 acagcacatt ctccaatact cttaccacca aaaggtccag ctttttcata actatttaca
    22321 aatgcaactt gacattttgg catatctaca gttctaaata atttatatgt tttaaaatca
    22381 gtagctttag gtctaccttg ttcatcaaat gccaattctt cactcaatgc gtatcctaga
    22441 cccatatgaa tcccaccttc taattgacct tctaaattta aaggatttat aactctacca
    22501 acatcatgta cagctacaaa atcaactact tttatatcct tagtttcttt atctactaaa
    22561 acttcagcaa aatgtacacc ataagaagta agtccagctg gtgaagaata atcttcaaca
    22621 acagttaact ctcttaatga tttactttga ctatatactg cgacatcaga aagtgatgct
    22681 tttacatttt catcaagttt actaattaca tatccattgt ctagatataa atcttctttt
    22741 gatgtttcaa gtaatttttc tgcttcttct actataaggt ttttcaattt ctctgctgtc
    22801 tttttagctc ctccaccttc tacaaatacc cctctacttg catagtcacc aaggttgaat
    22861 gtacatgcat ccgtatcagt ttcaaatgta tcaactttat caggtgttat tcctaaaact
    22921 tcagctacca tcattgtttg catagttacg acaccattac ccatatcatg tgctcctgtt
    22981 agtagtgtca atgtaccatc ttcatttaat ttaagagtca atgtaacaac atctctatgt
    23041 gcaccaaaaa ctccattacc atgtgcacct attgccatac ctatacctct taaatatttt
    23101 ccttcttctt ttggaagttt tttcttttca tcccacttaa acatcttctt accttgctct
    23161 atacaatcca atactctagg atttccaaga gaaccgcgga agattatatc gacatcatct
    23221 ggctctactg catttttatt ttgaaaatcg actaaatcca tacctatttc cttggctatt
    23281 ttcgcaaatt gagcctgttg agccataaat atttgaggtg acccatagcc tctcatagct
    23341 cctgctattg gtgtatttgt atatacaggc atcccagtaa actttatatt tggtatttta
    23401 taaactttaa atactttatg actaagtgct cctataacat ttaaagcact tgatgcatat
    23461 gctcctgtat ttgtatatat ttttatatct tgtgctatta cttcaccatc tttatttaca
    23521 ccagttttta attttacaaa agacccatga cgagttcttg ttgatatcat agattccttt
    23581 cttgtaagaa ccattttaac tggtcttctt gtcatttttg ataatgcggc tgctactggt
    23641 tcaattgtaa gttctaattt tccaccaaat gagcctccaa tagcaggtct tataactctt
    23701 actttattta atgacatccc aaaaatttga gatagtatta ttctaaatcc aaatgtattt
    23761 tggcaaggtg tatgtacagt taatttatca ttataatcat agtgtgcaat tacactatga
    23821 gtctctattg cactatgatg tattgctgga gtagagtatt catcttcaaa tatatagtca
    23881 cattccttga agcctttttc tacatctccc ccatttatgt tgttttgaga aactatattt
    23941 gaatctccat gtatgcagat agcatcttct tttattgcat cttgtatagt aaatacagct
    24001 ggcaattctt catattcaac ttttataagt tttacagctt tctctgctat ttctggagtc
    24061 tctgcagcta ctgctgcaac acggtcacca acaaatctaa ctgtatctga aaatataact
    24121 tcattttcag gtatattgtg ctcataaaat cttaatgaac cattgtattt tctttgtgat
    24181 gtattgagat aagtagctat agctctaaca cctactactt tctcagcttc acttgtatct
    24241 atacttttta tttttgcatg tgctacagga cttaaaagca tcttagcgta aagtacattt
    24301 tgtggtttca tatcagccac gtatttcatt tgaccagtaa cttttaaagc tgcatctttt
    24361 agagggtatg attttcctat taccttttcc ataattatct accccctaat ctagctgcag
    24421 ataattttac agcttctata atctttacat atcctgtgca tctacagaga ttttctttaa
    24481 aagcttcttt aatttcatct tcattaggag atgcatttct atttaaaaga gcttgtgttg
    24541 ttataatcat accaggagta caatatccac attgtacagc tccaacttct ataaaagctt
    24601 cttgtattgg atgaagattt tctccattag caaaactttc tatagtgtat ataactttac
    24661 cttcgcagtt ttttgcaaga agtttacaag atttcatgtc aactccatct atcaaaactt
    24721 tacatgctcc acaactgcca ctattacaac cttgtttcgc tccagttaga tttaattttt
    24781 cacgaagtac atgcaaaaga gtttcattct tttcaacttc tatttcatac atgtaatcat
    24841 taatatttaa ttttattttt tccattttct cctcctacag ataaactaat gttgtaatag
    24901 aatattatta caatcttatt ttaatattag tataagctat tccacttatg tccttccatt
    24961 ctattagtta cattagctct cttacataat tcttctgctg caagttgtgc attaagcaac
    25021 acttgttctt cactttcaat tgtagttact tttccatttt caagtattac atttccatca
    25081 actaaaacac tctctacatt gtgcatacta gctgagtata ccaaagttga tacaggattg
    25141 tgaagaggta ttgattttgg agaaagcatt gagttaaata ttactaggtc agcttttttc
    25201 ccaatctcca aagaacctat ttcatcttcc attctaagag ttcttgctcc ctcaattgta
    25261 gccatttcaa gaactttctc tgcagaaatt gccagagggt cacaattatt tactttgtgt
    25321 tgtaaagctg ttaatttcat aagctctacc atatcttgtg cgttattact tgctgcaccg
    25381 tcaagaccta aactcacatt tatacctttt tttaacattt ctggcacagg tgcaacacca
    25441 gaagcaagat acatattact tgctacatta tgagaaactt ttatgtcata ctttttaagc
    25501 atttccatgt cttcatcagt tatacataca caatgtactg ctacaacttc tggacctact
    25561 ataccccatt tttcaagtaa ttttatatct atttctccat gtatatcctt tgttgctgtt
    25621 cttgcaaatt ctgtttcaga tatatgaact gtaaaatatg aatcatattc tttaactata
    25681 ttccaaagca tcttacccat ttcttcagat atagcccaca ttgaagaagg agctacacag
    25741 atttttattc ttccattttc tgaattatga tatttttcga atacccttct caaatctttt
    25801 tcaaccgttt ccacatcttc tataagctct ttgtgaatac ctaaatctat acaacctcta
    25861 cctacaaccc ctcttatacc taaatcttta tatgccttta ctattccatc tgtaagtcct
    25921 ggtctattgt gagtatgcat ataatcaacc attgtagtta taccactttt taaaccttcc
    25981 atacatccaa gcattgcagc atcataagta tcttgttctg ttaagaattt tgctgatgga
    26041 aacatcattg tatttaacca ctcatgaagt gccatatcat cacctaaacc ttttaataac
    26101 acttggaaca ggtgattatg agtatttata aagccaggga atattacttt tccattagca
    26161 tctataactt ttttagcatc aagatactta ggctcaatat caactgtatt tcctatatcc
    26221 aagattctgt caccttgaat agctattgct ccatcaaata taacatctct gtctttatta
    26281 actgtaacaa ttattgcatt ttttattagt atatcaatca tcttaaatcc ccctttgaat
    26341 actttcatta caacatttta ccatatggtt ttctcatatt atatctaata catttatcca
    26401 atattttcct caagatttga agtaacgttt tcagcacttt tacgtgactt taatttaaat
    26461 aatgtttgaa gtaatatagc tattattgct ccagaaacta tacttgaccc tccaaggaca
    26521 gttccaattg tttctgggaa ttggtctaaa acttgtggag cagttgtaag acccatactt
    26581 gttgctatag atacacctgc tataagattg ttttcttcag tgaatccatc catactcata
    26641 atagagaaac cagacattgc tattgatgag aaaactacta aagttgctcc accaacaaca
    26701 caagatggta tagttgtcat tattgcaccc aatgctggag atatccctgc tagtaataat
    26761 acaagtgccc ctagtgctat tacaaatcta cttgtaactt tgttcataga tacaattact
    26821 gtattttggc taaatactcc agttggaaga gctgcaaata gtgatgaaat tatagaagtt
    26881 atagaatttc ctaaaacaac tgatgctaat tcttcgtcag ttacctctct atccattgct
    26941 cctactgtag ctattgtaca ggcacccatc atatcagcta tacctattat gtaaataata
    27001 gtaaacatta ctattaactc tggtctaaat tctaatccaa aagctgctgg tgttggtaaa
    27061 gatactatag ctgctccttg aacggctgag aaatcaacca atcctaaaac taaagatata
    27121 gcataaccta ctaatattcc tattaatatt gaagaatttt ttactaaacc tttaccgtat
    27181 ttatttatca ttattattac aaaagcaact ataactccta ctgtgaagtt tattgggctt
    27241 ccaaatgttt gtgtgccttc tcctccagct aaattcttta tagctgttgc gaatagtcca
    27301 agtcccatac atgctattat agttccagaa actattggag tgaatatatg tctaattttc
    27361 ttaactccaa atcctacaaa gaatacgatt accccagcag caatctgtgc tccaaataga
    27421 gctgctaaac catctttagc tgctactgaa agacaagctc ctaaaaatac ataactcatt
    27481 cccatcatta caggtaatct tgaacctaac ttaaatcctt taaataaagg tattggatat
    27541 aactgtaaaa acgttgctaa acctgctgct aacatagaac cctgaatcaa cattgttgct
    27601 tgcccctggt taagtcctac cacgtttgct attaatatcg caggtaccat atttcctact
    27661 atcattgcta acagatgttg taaggccaaa ggtattgatt cctttagtgg tggtatccca
    27721 tcgacatcat attttgaagt attacgtgtt ttcataaaat aaatcctccc atacataaat
    27781 atcttgacta aatattttta taattatttt ctataaatat ttttatgacc atgaatttat
    27841 tgctcaaccg ataaacacag ctaaactgag ctatttttat aaaaaattaa caaatttcta
    27901 aatattccct ccccgatttt caacttaaat gaaacattaa ttgcctggta tatatttatt
    27961 tgttaaattt tttattaaaa tgaatagcta tcaaaaataa tctaacaatc tcatactaaa
    28021 taatttaaac aattctttta aattattata tcttaattag tctcttccat aacagctctt
    28081 tctttagcct catcctctag acttttattt ggtattatta gatttaaaac gaatgcaacc
    28141 aatgttgcaa ttactattgg agattctcca aatactaact ttacaaatga aggaaatgcc
    28201 tcttgggctg ctggtactga tgttattccc atgcctaagg caattgccaa accaacaatg
    28261 gtaatatttc ttgatgacaa ctcttctgta ataattaact ttatacctgt cattgttatc
    28321 atactaaata tagatattgt agctcctcct aaaacagaat atggtatagt agtcattaat
    28381 gcaccaaatt taggcacaaa ccccgacaat aacataaata taccagctat tccaagcaca
    28441 aatctactta ttactttgtt catagctacc atccccacgt tttggctata tgatgatggt
    28501 ggtaaacctc caaacaaagc tcctatcata gtacaaattc cacttcctat tactccactt
    28561 gataattcct tatctgtgat attcctatcc atgcctccca ttgtagtagc tgataaatct
    28621 ccaattgctt gaacagcatt gattatactt acaattacta ctgagattat tacaggcata
    28681 tcaaattcca tcccaaattc aagcggttta ggtatcgcaa accaagttgc ctctctaata
    28741 gggtcaaaat ttattatacc cagaactaaa gatacacaat atccaactat aattcccaaa
    28801 aatattgctg ccaattttgc atatcctttt gcaaattgac taagacctat aactaccact
    28861 aatgttatag ccgctactgc ccagttttgc atagaggcat aagtaggact atttacacca
    28921 cctgccatat aatttattgc tataggatac aaagataatc caattgtaaa aaccactgtt
    28981 cctgcaacta tattcggaaa aaattttctt atctttttta tgaatatccc tattaatact
    29041 gtagccactc cccctattaa ttgtgcaccc aaaatgcttg ctaatccata atttcctccg
    29101 actgctacaa gtgttggtac ataagtaaaa ccaactccaa aaattatagg caatcttgaa
    29161 ccaatatttc ctattggata tagttgtata agtgttgata taccagctat tattaatgca
    29221 tattgcacta aaagtgttat ttgtgtaggg ctaacaccca cagctcctcc tacgactata
    29281 ggtactgtaa cagttccaac tatcattgct agtatatgtt gcattgatag tggcaaacac
    29341 tttaaaattg gtggccttcc atcaatatca aacatagaaa cattcttttg atatttactc
    29401 aaggttatca ctcccaacta ttttttaatg acagttatta ttatctcaca caacatcctt
    29461 tcttttttta ttttgtcatc tcaatttaat atgaagcaat tataatgcca attttttaac
    29521 ataattgttt tacatatata atttttatta tatttgttag tatttgataa tttattaaag
    29581 catttatttt tttaaattct tttaaatttt ttttattttt ggataataaa atgctattac
    29641 acaaaaaaac acttagaaaa tagttttcca agtgtttttt atcaatttta taatttcaaa
    29701 ttttagaatt gagaataaaa aacctaatta ttatacatct atttttctgt acaatgtagc
    29761 tagtgatatt ccaagttttt tggcagccag ttttttagac ttagtatcat ttccgtaaaa
    29821 ctttaacact ttctttatat aggctctttc aaactcttca agtgtaagta tgtcttgttc
    29881 catacttatc tcatagtcac tttcctcaag taactttata tttatattat ttaacttaaa
    29941 atattcaagt atctttctat ttattattcc tttatgtatt attccatctt cctcaagaag
    30001 gtttaatgcc agttctatag tattttccaa ctctctaata tttcctggcc aaggatactt
    30061 aagcaacatt tgttccacat ctggttctat atggtacacg tttttcttca tttttctcga
    30121 atatttgtct ataaaattat ttattaatat ttgtatatcg tctcctcttt ctctaagtgg
    30181 aggcacttct aaaggtatta cattcaatct atagtataag tcttctctaa atttattttc
    30241 cttaaccaac tcaaataaat ctctattagt agctgatatg attctaacat ctatatcaat
    30301 tagttcattt gaacctattc tcgtaaactt cttttcctgt aataccctca ataactttga
    30361 ttgtagttga attggcatat ctcctatctc atctaaaaat acaacccctc cattggccaa
    30421 ttcaaactta ccaattttac cttttgaatc tgctccacta aaagctcctt ttgtatatcc
    30481 aaaaagttca ctttctagta aaggttctgg tatagcagca cagtttatag caacgaatgt
    30541 tttattccct ctgtttcctg cgagatgaat ggctcttgct attaattctt ttccagtgcc
    30601 actttctcca gttatagcta cagtagaatc actatttcca attttgcaaa ttctattctt
    30661 aagagcacgc attacatcag actctccaat tatggagtct actgttgttg ttccccaaat
    30721 attatatact tccattccac catacattac tgaatcactg tgtttgtcca taaaatatca
    30781 actccaaata agtattctat ataataatca ttcacattat ctatttaatt atatcataac
    30841 agaaaagctt agttataatt taataatatt attttaaaac aaaacttaat aaaaagtata
    30901 tctacttagt tttaatttga tagatatact ttcttcattt
//
LOCUS       DAMKPG010000040        30675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMKPG010000040 DAMKPG010000000
VERSION     DAMKPG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.043"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            454..1146
                     /locus_tag="PC621_001933"
     CDS             454..1146
                     /locus_tag="PC621_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109092.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1342..3402
                     /locus_tag="PC621_001934"
     CDS             1342..3402
                     /locus_tag="PC621_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF4109093.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3608..3931)
                     /locus_tag="PC621_001935"
     CDS             complement(3608..3931)
                     /locus_tag="PC621_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF4109094.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3924..4625)
                     /locus_tag="PC621_001936"
     CDS             complement(3924..4625)
                     /locus_tag="PC621_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF4109095.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4903..6288)
                     /locus_tag="PC621_001937"
     CDS             complement(4903..6288)
                     /locus_tag="PC621_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF4109096.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6396..7634)
                     /locus_tag="PC621_001938"
     CDS             complement(6396..7634)
                     /locus_tag="PC621_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF4109097.1"
                     /translation="MELFIEVIKFNIYISLLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRL
                     NSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(7647..8015)
                     /locus_tag="PC621_001939"
     CDS             complement(7647..8015)
                     /locus_tag="PC621_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4109098.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KILSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
     gene            complement(8543..8965)
                     /locus_tag="PC621_001940"
     CDS             complement(8543..8965)
                     /locus_tag="PC621_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF4109099.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            9443..12346
                     /locus_tag="PC621_001941"
     CDS             9443..12346
                     /locus_tag="PC621_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109100.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            12379..12825
                     /locus_tag="PC621_001942"
     CDS             12379..12825
                     /locus_tag="PC621_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4109101.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            12948..13250
                     /locus_tag="PC621_001943"
     CDS             12948..13250
                     /locus_tag="PC621_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4109102.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            13431..14837
                     /locus_tag="PC621_001944"
     CDS             13431..14837
                     /locus_tag="PC621_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF4109103.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            15181..16239
                     /locus_tag="PC621_001945"
     CDS             15181..16239
                     /locus_tag="PC621_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF4109104.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            16314..17000
                     /gene="araD"
                     /locus_tag="PC621_001946"
     CDS             16314..17000
                     /gene="araD"
                     /locus_tag="PC621_001946"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF4109105.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            17018..17665
                     /locus_tag="PC621_001947"
     CDS             17018..17665
                     /locus_tag="PC621_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF4109106.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(17838..19205)
                     /locus_tag="PC621_001948"
     CDS             complement(17838..19205)
                     /locus_tag="PC621_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF4109107.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(19395..19483)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19911..22031
                     /locus_tag="PC621_001949"
     CDS             19911..22031
                     /locus_tag="PC621_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF4109108.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            22135..23388
                     /locus_tag="PC621_001950"
     CDS             22135..23388
                     /locus_tag="PC621_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF4109109.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(23542..24603)
                     /gene="ytvI"
                     /locus_tag="PC621_001951"
     CDS             complement(23542..24603)
                     /gene="ytvI"
                     /locus_tag="PC621_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF4109110.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            24877..25617
                     /locus_tag="PC621_001952"
     CDS             24877..25617
                     /locus_tag="PC621_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF4109111.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            25629..27407
                     /locus_tag="PC621_001953"
     CDS             25629..27407
                     /locus_tag="PC621_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4109112.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            27574..28491
                     /gene="pfkB"
                     /locus_tag="PC621_001954"
     CDS             27574..28491
                     /gene="pfkB"
                     /locus_tag="PC621_001954"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF4109113.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            28528..30447
                     /locus_tag="PC621_001955"
     CDS             28528..30447
                     /locus_tag="PC621_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4109114.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        12115 a         3362 c         4976 g        10222 t
ORIGIN      
        1 atagaatgta taaaaataga atttaaataa gtagctatta ttgcagtgaa ataaatgttt
       61 ctaagtatat ttgggtggaa gaatggtgga agaaaattag tgaaatgtaa ttcttccacc
      121 attcttccac cccaaaaata aaaactgttc aataagaatt aatatacatt aattcagctg
      181 aatccaatca aaatacttaa ataaaagtaa tctgaaaata acataattca aatcaattta
      241 ttcaaataaa tctcaattta tcttaaatct taagaaatat ttaagaatta ggataatatt
      301 ttcttaagat ttatatttta ttatatagtt ataagggaaa gttacaaaaa tgtaagccaa
      361 agtaatataa caatttagtc attgttgaga aagtaagtga aagatattgt ataatataga
      421 taagtattat tatacaatag aggaagtgaa aatatgtaca atatattagt agtagacgat
      481 gataaagaaa ttgttgattc aatagaaata tatcttaaat cagaaggatt taaaatatat
      541 aaagcatatg atggaatgaa agctttggaa ataataacag agaatgacat tcatctaata
      601 ctaatggata taatgatgcc taaattagat ggaatcaaag caactgtaaa aatacgagaa
      661 gaaagaaata ttccaataat tttaatttca gcaaaaagtg aagatacaga taaaataata
      721 ggattaaata ttggagctga tgattatata acaaagccat ttaatctatt agagcttata
      781 gctagggtta aatcaaactt aagacgatat gtaactttag gtaattatga agatacatgt
      841 aaagaaattt taaaaagtgg agctttggag ttaaatacac taacaaaaga agttaaaatt
      901 gatggtgaaa acataaaaat gaccccaatt gagtataaaa taattgaact tcttttagaa
      961 aataaaggac gagttttctc aatagatgag atatacgaaa aagtatggaa tgaggaaagt
     1021 tttaattcag aaaatacagt agcagtacat atcagacgaa taagagaaaa gattgaaata
     1081 aatccaaaag aaccaagatt tttaaaggtg gtgtggggag ttggttataa gattgaaaaa
     1141 ttttagtgga aaaattataa aagaaaagag tttaatattt aattgcattt tgattttagt
     1201 catttttaca tttacacaaa tgttaggaat tttaatatgt tttaattttt tctctgagag
     1261 tttaatttac tctatgggga ttatgctttt cttaaacttt attttatata aaatactgac
     1321 aatgtagatg aggtgggcaa cttggaaaaa tatagagcat taaaattagt ttcattaatc
     1381 ataatatcta tctcatttcc attaactata ttgttggcta caataggact tgctgagcct
     1441 aaatacaata tacaaaaaac ttctacaaag agtgggattt ttaatatatc taatgatttt
     1501 tatgatagta atatatttga tgaaggaaca gtggaaagct tattatctaa ttttaagata
     1561 tctgtggata gtcagttaac tagtgaagaa aaaagtaaac tccaaacaaa aagtaaaagt
     1621 atagatttaa gtagtaatga ttctgagtat gataaaatca tgattaataa acaagcttct
     1681 gaagaatatt taagttatct aaaaaatata aaagtgtatg ttattaatac atatacagga
     1741 gtattttata caaatacatc atatggtaat ttagatgatt ttaaacaaaa aactaagagc
     1801 tattgtaata tagagataat aaataatgta ggaaagagct cgtatattaa aacaataaat
     1861 ggagaaaaat ttgaactgaa taatctgagt atggaactaa atggagtaga tgaagccttt
     1921 gaggcatatg tctcttttcc agaagagcca actatagaag atggaatagt atacactaat
     1981 tttcaaatat ttaaacaagc tacagaaaaa gtaagattat tttttgtagc ttgtttaagt
     2041 ttagcaattg tgtgtttatt atctatcatt ttatatagca gaataaaagg tgaagtttta
     2101 aaaagaaata atgtattttt actaatatac agtaagatac ctatggaatt taatgtaata
     2161 ctagtattta tttcaattgc aattttaact atgtatgctc ttgctgtatc ttatcatatt
     2221 gatgtaattg tattagtttc tttatcttta ctgatagtat caaataatat attttttata
     2281 aatgtgcaat tgagttattt agaaaataaa atgtgtatat ttaagacaag tattatagta
     2341 agagtgattt tatggttaat aaaaactgta agggaggtca gtaacgctat aaagaaagta
     2401 tctttagcaa ggaaagttat tgttatagtg ataatatctt tagctttaat catggtaatt
     2461 agcactcgaa gtataggtat gggattactg ttttctatat gtattttttt aggatttaca
     2521 atatatataa ctaaaagact ttcatatctt agttacgtaa tagaaggtac agaaagaata
     2581 aaaaagggag aattagatta taagataaaa attactggga atgataattt tacgtcttta
     2641 gctgaaaata taaataatat tggagaaggt cttgatagag ctatagaaga gcaagtaaaa
     2701 agtgaaagaa tgaagtctga tcttataaca aatgtatctc atgatttgaa aactccactt
     2761 acttctataa taaattatgt agatttaatt aaaaaagaag aatcaataca accagagtat
     2821 ataaatgatt atataaaggt acttgagtca aagtcaaaaa gactaaaatt attgatagaa
     2881 gatttatttg aagcgagtag agtaagtagt ggcaatatag aattacaaat tagcaagatt
     2941 gatttagtgc aactattaag acaaagcata ggtgaacttg aagaaaaatt atctaaaaat
     3001 aatttatatt taaaattaaa tgttccaaat gataaggtgt atatatgggc agatggaaga
     3061 agaatgtata gagtatttga aaatctttta tctaatatag caaaatattc cttagaagaa
     3121 actagagtct atattgatgt atatgactat ggtgaaaatg taaaggtaac tatgaaaaat
     3181 atatcttctt atgaattaaa ttttgaccca agtgagatta tggaaagatt taaaagagct
     3241 gatgaatcaa ggaatactga aggaagtgga ttagggcttg ctattgcaag agatttagta
     3301 gctttacaag gtggaaaatt ttatatagaa atagatggag atttatttaa agcaaattta
     3361 gagtttcaaa aatatgaaga agaaataaca aatatggaat gagatagtat ttaagtatat
     3421 atattttttt tactgtcgaa agcttttatt ataatatatt agaatattta cagtagagaa
     3481 tgaagagtaa aaacagagta actttaagtc actctgtttt ttatttaaaa tcaataatat
     3541 aacaacttaa attaaaattg tacacatagt tttaaaataa cttatacaat aaaacattat
     3601 ccaaatatca caagataatt ccaaataaat atatagatat cactcctgta ataatagaaa
     3661 taccaataga tttaaactta tatgcaacaa gaagtacaat cataaaagat ataattttag
     3721 ggttagctat agataaatat agactatctc cattaaaaaa tacatcttta gcagttaagg
     3781 aagtaagtat tgctacaggt ataaatttca tgattttttt caaaaaatca ggcatctctt
     3841 tttttgaaaa aattaacata ggaagcactc ttggtagata tgtaacaatg tacattccaa
     3901 ttataataaa aaatatataa ttattattca taagattcct cctttttaca gtaagattct
     3961 tctataaaga aaccaattaa agatgctaaa attgatgcaa ctattattac taaattgctt
     4021 ttgaataatg catataaaat aacagaaata atgattgatg atataaatac tacaatataa
     4081 atcttatttt ttatttgaga aactattaaa gctataaaca tagaagtcaa tatgaagcca
     4141 gaaatagaac tgtctataga tatactttct cctaaaaatg ctccaataaa attagctaaa
     4201 actgatgaaa ttaagcagaa gaaattaaga taaatagcat ttctatcaga ccaatttcca
     4261 gcagtaaatt tttcataatt aactgcaaaa gtttcatctg ttatagtatg acaaaatggc
     4321 aatataaatt ttggactttt attttttatg tacatgttca aattagaact cattagaaaa
     4381 tgtcttaagt taactataaa tgtagtcaaa acaatagatg ttacacttgc accagtagaa
     4441 atcattgaag ctgcaatgaa ttgagaactt cctccaaata ctaaagttac cattagtgct
     4501 atttgaaaag gtgaaagacc agatttttgt aatagtattc ctcctactat acctataggt
     4561 atataaccta agaatatagg aattgaagca ataaagcttt cttttaattg ttgagttctt
     4621 tccacaataa atcactcctt ttgtattaaa tgtattaaag tattagtaaa acagaaaaat
     4681 agattaattg tataattgca attataatta aaaataaatt tggattaata tataaaatac
     4741 tatacaacaa ataaataatc ttaaacggtt attaaatttt atctatttgt tattatgatg
     4801 taataataca tattaatctc tataagttaa ttactatctg gtgtaatttt attttaacaa
     4861 caaaaaaaaa tacacacaat taaaatattg tgtgcaatac aattattttt tctcttctaa
     4921 tagtcttttt aatacgctca gaccagcttt aagttcttct gtgctttctg tagcacatac
     4981 agctattctt attgcagaaa ttggtttttc ttttccaaca gcaaaacgtt cagaagcata
     5041 gacctgtaca ccttctttaa gtgcttgtat ctcaaatttt tctgccatta ttccatcagg
     5101 tagaatcaac catctaaata tacattcttc atttcccaaa agattatatc cagataaata
     5161 ttgattaata atcttgtttc ttcttcttgc agatttacgg cgtgcattaa ttaatttatc
     5221 ggcttcacct gatgtaatca tgcgataagc aatttctgtt aaaaaataag attgagatag
     5281 attaatgttg tataatgcgc ttgataatgc ctctcgatac tgtttaggtg tagagatata
     5341 tgctagccgt aaactaggtg caataatttt tgataaactc gtaatataaa ttgtttgatt
     5401 gggcagatag gaagccactg gatttaaatg tgtttcatta agtaaactat gaatagcatc
     5461 ctcaataaca attaaattgt atttagatgc aatatttgca atcatttttc gtccattttg
     5521 tgacattata tgtgtagttg gattttgata atctggtatt atgtatagac ctttaatatt
     5581 ttcattttta caagcatata atagtccttc ttcactaatc tcattatctt cttgtttaat
     5641 tggaattagt tgaatcccaa gcattttagc tgctgtcttt attccaggat aagttaatgg
     5701 gtctacacct attctatctc catgttgaaa caaaccagct aaaatagcaa caattgcatt
     5761 ttgtccacca cttgcaggaa gtaagctatc tgatgtagtt tcaagcccag ctttataaat
     5821 taatttggca atagcttctt tttgccattt agcaccatct ctttgtccat attggaatat
     5881 agttttaaaa tctatttcct taattatatg tttaaacaaa gtattcattt cttctagtgt
     5941 gaaatctgga ttcattgtgc ccatttcaat aaatgtaatt ttattattac tggacatcaa
     6001 gaatagattt gaacgcgtat cataagatac aaaagttcca cttcctgtaa ctccacttat
     6061 tagacctttt ttttcacaaa ttttaaaagc tctagagata gtactcacat taacatctaa
     6121 gaaatctgcc aattcacgtt ggggtggtaa tttagtacct ggtaataaaa atccattgtt
     6181 gatatcttgt tctagttgtt ctgcaagcgc cttatataat atttgccctt ttgaaggtct
     6241 ctttggtttc cagttcatag gataattttc aaaagaatta attggcataa gttttcacct
     6301 cacattatta atttgatttt ttctttatct aaaacaataa attctaaaga tacaaataaa
     6361 aactgatagg aaataattct atcagttttt gtcttttaat tgatttcaaa tcttcctata
     6421 atttttatac cttctttatc ataaagaggg attgtttttt taaatatatt aaaatttctt
     6481 tttcttgtat ataaaacagc ttcttctggt gtctttggtt gattatcaca atcgtacaaa
     6541 tctctttttt ttacgtagcc gactttttta cctctaccta ttgtttgtat aagttctggt
     6601 tcaatggaat ttccatttga gtctattata tgtgtaccat aggtttctcc atttttatta
     6661 taattaatct tagcaatagt atattctaca tcttcatttt gtttaaaatt ggagttgata
     6721 gaaaatattg accctattat tataaaggtt aataaaaact taagacctaa attaattttc
     6781 tttttatctt gattaaacat agaatttaac ctctttttta aaataatatt ttttttattt
     6841 agaaactgag atacgaatga tgaatacttc aaagtttttt gatactttat tgtatttaat
     6901 attatatatg catactcttt aatttcactc gtggtacatt tgtcaatcac aatttcatca
     6961 caagataatt cacataattc atgaaaatat ttttttaata agtataaaat tggattgaac
     7021 caatgtatga tacatattat actaattaga agcttaaata agttatcttt tcttttataa
     7081 tgaattaatt catgtttaaa gataaatctt aactctttat tattgtactt tttttctgga
     7141 attattattt tacagcgtgt taaacctata atggcagggg aataaatacc tttaacctta
     7201 tatatctgaa gttttttatt tatacttagt tcttttgctt gttcatctaa gatactagta
     7261 atatctattt ctaaaatttc ttttttaaaa ttttctatgt aattataaaa tttaaaattt
     7321 gttcttaaat agtaaaaaga gattgctata attcctaata tccagatgta cataaatata
     7381 tctaaagtaa tgccctggtc atatggtata taattatcga gattatgttt atagatatta
     7441 atagtagtat tattgatatt taatatattt aaatttattt tagatataaa catcattctg
     7501 actactatta aaagacatat aaaataatta aacttaaaag tatatttgct tatcttttta
     7561 gtaaataaaa tgaaaaaaag aattagtaga cttatgtaaa tattaaattt aataacttct
     7621 ataaataatt ccataaaata cgtctcctat tttctttttt ttagccattg ttcaagttct
     7681 tttatgtcat catctgatat ttcatttcca caacctaatg atgatataaa gctatgaata
     7741 gaatttttat gtataagctc taaaaaatcc tttgtttcaa attttagata ttcctcatgt
     7801 ttaactttaa tagtgtataa agtatttcta ccatcttttt caaattctaa aaaaccttta
     7861 tcacttaatc tagaaagtat tttaccagta gtaccttttg tccatccatg agaaatttcc
     7921 atataattag ctacttctct ggctggtatt ttatctatgc ctttatccca tatgaaattc
     7981 ataactaaca actcggattt tggtattttt ttcaatataa tcactcacct ttaacttgat
     8041 tatattagac aaatgtctaa tagtcaatat aatatatcat catgtataaa gaatctttat
     8101 attattttat gcttgagaac taagtagtat agatttatag ataaaaaaga tattgagagt
     8161 tttatggaag aggggaatat attttctatt agagataaga atatgacata tattttttat
     8221 tgatttgtac aaaaatacta tatagtgaat aaattaatat cttttctaaa ttagaagagg
     8281 ataatcttat tagataaaga ttatcctctt tattgaaagt ttaagggata ttagagatta
     8341 ttaatttttc tctctttttt taactaaaag caataataca taaaattatt attgctaatg
     8401 atactcttaa tgtggtatat agtcatagtg tcacatgttt atcaatagtt ttttcattaa
     8461 ttattgtaac gaatagtagg actcataaaa tacttagaaa tatgttttta gatagatttt
     8521 aaatatataa tctaacttat atctataaat taacactatt attctccaat tccatttcta
     8581 gtttttttat aaatttatta aattcttctg gttgcctttt tccaaacaat actcttacaa
     8641 gagctgaatt gatttttcca aatatattcc tcttgctctc actttcaata aaaataagtt
     8701 ttgtattttc taagtcaata tgtactaatt catatctagt aatagtactt tctcttttat
     8761 tgcttgtttt tatctcatat actctatttt tttcgaaatt agttatttca accttaaatc
     8821 ttttattatt tttttgttgt acaaatgttc ctactgcttt tttctcatta aaattaggaa
     8881 aatttagtct tgcagatttt gtaaatatat taaatatgtc ttttgcagaa tattttaata
     8941 aaacttcagt tttaaatgta ttcatatcgt tttcccttct gtatataaat ttaatattta
     9001 taagttaatt tttaagttaa aatagtataa tatgccatat taaatcatgt taaaactatt
     9061 tatatagcta aaaccttacc tatataagta tgtatactat catataaaaa taaaatctta
     9121 aaatagaaag ggttaaaaac acagtttatt ataattttta ttgttgagta gatacgtact
     9181 attatatatg attgagcaag tttttcaatc ataaatttat aaagtatttt gcattaaaat
     9241 ttaaacaaag agttaaatta tttaagatta tgaataacta atacatttag taaattacta
     9301 aatatacaaa atgtaaatac ttatcaaaat gtataaatac ttatgacatc tagttactta
     9361 cagggtttaa gtagaaaaaa atatcaaagt aagtatggca tgatagttgc ttatatattt
     9421 atcaagaaag gagttattaa gcatggagtt gagagataaa cttgtaaaca tcataaatac
     9481 agaaaataaa aaagatccat taacagatgt tcaaatagca aagtttttaa gtacaactag
     9541 agagaacata actaatttga gaaaagaact taatataagt aattcaaggc aaagaagata
     9601 tccttattta aaatcagcaa tttcggcaat attacaaaag aataagaata tttctatatc
     9661 agaaattaca agggaattga tgacagaagg ctttaatata tcgagaagag ttgtggagga
     9721 acttttacct aaagaaagtt tagaaatgga tgtagtagag gaatctgaag aagaatcgaa
     9781 tgacccattt gaaactctga taggtaataa agggagtctt agaaatgctg tggagcaggc
     9841 taaatctgct atattgtatc caccaaaagg attaccaacg ttgattattg gggaaagtgg
     9901 agttggtaag agtttatttt ctagacatat gtatgagttt gcaaagcaaa aaaagatagt
     9961 aaaagaaagc tccaattttg ttgtatttaa ctgtgctgat tatagtgaca atccacaatt
    10021 attattatct ttgttatttg gatataaaaa aggtgcattt acaggtgctg aatatgatac
    10081 accaggtctt gtagaagaag ctgatgaagg agttttattt ttggatgaaa tacatagatt
    10141 gcctccaaaa ggtcaagaaa tactattttc tatcttagat aggggaaagt ttaggagatt
    10201 aggtgaaact aatgctgaaa gaaaagtaag tattatgttg attggggcaa ctactgaaaa
    10261 tgtagagact aatttattat taacattcag aagaaggata ccaatgctaa taactttgcc
    10321 accattacat gatagattac taaaagagaa aatagacctt atatacaata tctttcaaca
    10381 agagtgtaat agaataaatg caaaaatatt tgtagataaa aatgtgattg aaatacttgt
    10441 actaaaaaag tttagtggaa atataggaca attacaaaat atgattcagg ttctttgtgc
    10501 aagagctttt atgaagttta taaattccaa agatgaagat agtgaatcga tagtagtagt
    10561 agatataaat gaagttttaa agcttaaaga ttcttttaaa gatgctgcct ttcaagagat
    10621 tgaatataca gagataagaa aatatttgaa aaatgctata tttataccat ttaatttaga
    10681 agaatcatca gtaaaaggaa gtcttttaag tgatgaatat atattaccag aggacatata
    10741 caaaacaatt gaaaagaaat attatgacct aaaatcccta gacattgaag atatagatat
    10801 agagaatatt ttatggactt ttattttaaa tagatttgct gacttaaagt ttaatctcga
    10861 tagtaatgaa gaaatttttt caatgagtga tttaaatagt tttgtaagtg atagtttagt
    10921 taaactggta aaagaactta tgaatgatat agttgaaaaa aatatgaatt tagaagtaaa
    10981 taaaaatatg ttcaaatatc tagcaataca cttagaagaa gctataaaaa gaattaagct
    11041 aaatcaaaag ataataaatg taaatttaga aaaaataaag attgactttt caaaagaata
    11101 tgaaattagt aaaagttttg caataaaaat tgaggaatca attagtatta agattccaga
    11161 tgatgaaatt ggatttataa cactctacat aaagtctgct ctaaaaaatg aagtgaaaaa
    11221 agataaagta gggttaataa taatttctca tggacgtata gctacagaga ctgtaaatgt
    11281 agttaaagaa cttttaggtg taaagtttcc tgtggctata gatatgcctt tagatgaaaa
    11341 accaataaat atatacaata aggcagtaga actgtcaaaa ataatagacc aagggaaagg
    11401 tatattgttt ttggtggata ttggttcact tacaaacata ggtcaaatag taaataagag
    11461 aaccaatata agcactaaaa ctattgatag agtagacctc ctaatggcac tagaagcaac
    11521 tagaaaagtt tctatgggag aggaagaact tgatgaaata tttttttctt tgctaaaaga
    11581 taggatggga tataactatg aattagaaaa acacatagat aaatcaaatg caattgtaac
    11641 attatgtctt actggagaag gaactgctaa gtatataagt aaaacgttgg aagaaaagta
    11701 tgataacact aaatgttatc aaatgagtgc attggatgaa aatttatttt gtaaaattga
    11761 aaatctaaaa gagacaaaca atatattagc tattgttggg acaataaacc ctaagatacc
    11821 aggtataaat tttataccct ataataaaga acttttcaaa aatttggata tctatctatc
    11881 aaaatcaaaa caacaagatg acagaccagt taaatcgtat gaaagaatgc ttgatgagga
    11941 tttagtgatt tttgaaccag atatatattt taaaaaggat ttattagaat atgtatgttc
    12001 aattcttata aataaagaat atgtagaaaa agattactta gattctgttt tacatagaga
    12061 agaaatgctt ccaacatatt ctaaaggagc tataggtgtg cctcatggag attcttctac
    12121 tgtaaatagt acaaggtttg tttttgttaa actaaagaat cctattgatt ggggtgtagg
    12181 taatgtaaat ttcatattta tgccagtttt tcaggctaat gataaagaga ttgttaaaaa
    12241 tgttcttgag atactaaaag atgcagaatt tatgaacaat gccaataagt gttttgataa
    12301 agatgctttt agaaggatta tatttgataa atttaaacat ctgtagccat agcaaaaaaa
    12361 ttgagaggag ttagagtaat gaatgtagat ttaatatcat tgggtttaga atctaatgat
    12421 tatgaaagtg ttattgaaga attaggaagt ataatgtgta aaaaagaata tgtaaaagaa
    12481 acatacataa atgctgtctt ggaaagagaa aggacattac caacaggtct agatattgga
    12541 gaaatgtgtg ttgctattcc acatacagat tcaaagcatg taaatgagtc taatgtagct
    12601 gtagggatat tgaaaaatcc tgtaaagttt aattcaatga tagacccaaa agatagactt
    12661 gatgtagaac ttgtattttt attagcagta aagaacccag attcacaagt aaaactgtta
    12721 aaagatttaa tgtcagtttt tcaaaatata aagttattaa aaaatataaa aaacgcatca
    12781 acaaaagaag aagtagctaa attattggat tttatagaaa tttaatgtag gagaatttta
    12841 taaataaaaa attaaaattt caacaatcaa agtaagtatt atttttttaa taggtttaat
    12901 cttatataaa ataaaaattt agagtgaaaa attaggagga aattattatg aaacaattta
    12961 atgtattatc agtttgtggt tcagggacag ttacatcatc tatggtagca ggtaaattaa
    13021 aagaagtact aggtaataag gggatttcta ttactacaac agaagctaga ccaacagaag
    13081 cactaaattt agcacaatca ggaagatttg actttataac atttacgagt ccgcttcaaa
    13141 caggcgatta tggaatacca actataaatg catttgcttg cctaactggt ataggggaag
    13201 aggcattctt tgaagaagtt ttgactgtta ttgaaggcat agataagtaa gttttggaga
    13261 cgtgaattta tagtatttaa atcaatgatt tagatactat aataagctaa tattaactaa
    13321 atatttgtgg aaaaaacatt ttatatgttg tcataaatta aaaaattata aaaatagtca
    13381 cttaagtatt aaattaagct tataaatatt tataagtagg agggaaaaag atgttagaat
    13441 ttatgaaatc tataatcgat acatttggtt ctgcaattat agttccaatt ataatattca
    13501 taatagcaaa gatatttaaa gtaactacta agaaatcgtt tctttctgct gtgtatgcag
    13561 gagttgcttt acaaggtttt acactgattt taaattcatt tacaccaata ataacaccag
    13621 ttatcaatag gatggttgag agtacaggag taaacctacc agtttttgat gttggttggc
    13681 aagcaacatc attggttgca ttttcaacaa gtgcaggtat gatatattta ggtcttggta
    13741 tattattaca aacaatttta tttttgataa aatggacaga cgtgtttcaa ccatcagatt
    13801 tatggaataa ctattcttat atggtatggg gagcaatggt tataggagta acaggaaatt
    13861 ttcctttagg tatagcatgt atggttttat taaacttata tagtttatta atatcagagt
    13921 tagttgcaaa gagatggtct agttactata gatatcctaa ttgtacaata atagctatgc
    13981 ataatgttga ggcttctgtg tttgcagtat tttcagaccc tatatacaat aaattgggat
    14041 taaataaaat aaaattaaat ccaaaagaat tagagaagaa attagggttt ttaggtgaac
    14101 caattacttt aggtcttttt ttaggtatgt ttataggtat attaggtaat atgacgagaa
    14161 taaatactat ggaagcttgg ggagaaataa tgaaagtagg tatatctaca agtgcagtga
    14221 tggcgatttt ccctaaagta gcaagtatgt ttgctcaagc atttgcacct ataacagaag
    14281 cagctagaaa gattatgcaa aaagctggaa accgtgagtg gtacatagca gtaaatgatg
    14341 ctgttggata tggtgaacct gcaactttaa tatcaggtct tatattaatt ccaataatgc
    14401 ttgtaatatc tatggtactt cctggtaata aggtattacc agttgtggat ttgttggcaa
    14461 ttccatatat ggttcaaggt ttagttgcca tacataatgg taatatacca aaagtattag
    14521 tttcaggtat aatttggttt ggattaggat tatatgtttg tacatctaca gcaccattat
    14581 ttacagatat ggctacaaat ataggtgtag ctatacctgc aggagcaatg ttaataacaa
    14641 gctttaatat actaggaaaa cctttgatgg gattagtttt ctttgcattt ctaagtgcaa
    14701 atccaattta tataggtctt tcagttgtaa tttactttgt tctatgggca ttgtttagaa
    14761 agaacaaaac aagtattctt gattatttag aaaaacaagc acttaaaaat gtagaagaag
    14821 agcctgtagc agtttaagtt tataaaaatg aaataagaat aattaaagga gtttataatt
    14881 gattttatat aagtatcaga taatacctga gaaatattta aatgaaatat agacttcttt
    14941 ttatttgata atatttgtaa ttataatgca ttgtattttt atagttgagt tttctgtata
    15001 acctgctatt taaaacatac tcttttatat ttatacagta atgtggttta attttataaa
    15061 atgtaatctc tactggtaaa agactttgta tataaggcat ttgcctgaat aaaccattta
    15121 tcttttgtat ggcatagata ttgctatata gaagtatata gcatgaaagg agatatttat
    15181 atgggagata aaatgagagc atcagtattg tataatgtag gtgatgtcag gtatgaaatg
    15241 gtagatatac cagaaataac agatacgcaa gtgcttgtaa atgtaaaata tgttgggata
    15301 tgtggttcag atttgcctag gtctatggta agtggtttga gtggaaatac aaaatatcca
    15361 ttgattttag gacatgaatt ttctggtgaa gttgttaaaa ttggagaaaa agtaaagcat
    15421 attaatgtag gagatagagt agctgttgca ccattagtac cttgtggaaa atgtgattat
    15481 tgtaatgaag gtaattttgg cttgtgtgat gattacaata taattggaac aagagtaaat
    15541 ggagcgtttg cagaatatgt aagagttcca gaagaacata ttttaaaact accagataca
    15601 ttggattatg aaactgctgc cggaattgaa ccagcaacta tagcttatca tgggatttca
    15661 aagtctaata ttagagtagg agatagtgta gttgtgttag gatgtggtcc aataggtcag
    15721 tttgttatac aatgggcaaa ggtgtttggt gcatctaaga ttatagcagt tgatattttt
    15781 gatgaaaaat tagaactttc taaacttcta ggagcaaact atatacttaa ctcaaaagaa
    15841 gtaaatgtaa tcaaagaaat aaaaaaaata acaaatggtg gtgctgatgt tgtaatagag
    15901 actgctggta gccgatttac acaagaacaa tctctatttg tagcaaaaaa gagaggaaat
    15961 atagtgtttg taggtatatc acacacagaa ttaccactta gtgcagacgc tactgagtgt
    16021 atattacgtg gagaacttac cttgaaaggt tcttggaatt catatgcaag cccttatcct
    16081 ggaagagcat ggactgcaac attggatttt atgggaaaag gtgacattat ttttaaaccc
    16141 atgatttcac ataaaatagg tcttaatgaa gtgggtgact ttttaagtaa aatgagcaag
    16201 agagaaatta atttcaataa aatcctagta gaaatataaa aatttaataa aaaattctaa
    16261 aaacaattaa attttataac tatgacatta aataaaatat aggaggaaga aaagtgctag
    16321 aaattttaaa agaagaagta ttaaaagcaa acttagaatt acctaagaaa aagctaatta
    16381 cttatacttg gggaaatgta agtgggatag atagagaaaa aggattagtg gtgataaaac
    16441 caagtggtgt agagtatagt aatatgactg tagaagatat ggttgtagtt gatttagagg
    16501 gaaatgtagt tgatggcaaa ctaagaccct cctcagatac tccgactcat ctagttttat
    16561 ataaagagtt tgaagaattg gggggaatag tacatacaca ttcaagttgg gcaacaattt
    16621 gggctcaaat aggaaaatct ataccatctt gtggtactac tcatgcagat tatttttatg
    16681 gaagcatacc atgtaccaga aaaatgacta gagaagaaat ttgcaatgaa tacgaaaagg
    16741 aaactggaaa tgtaatcatt gaagaattta tgggaagaaa tccaattcat tgtccaggaa
    16801 taattgtaaa tgaccacgga ccttttacat ggggaaaaga tgcaaatgag gctgtacaca
    16861 atgcagtagt tttagaagaa gtcgcaaaga tggcttatta tacagagtta atgagtcctg
    16921 ataatattat ggataaggtt cttatgaata agcatttttc aagaaaacat ggaaaaaatg
    16981 catactatgg tcaaaaatga ttatctggag gtgaattatg ctagactctt taaaaggtaa
    17041 agttgtggaa atagcaatta aagcagagca ttatggttta tgtaaagaaa agactggtaa
    17101 ttttagtatt agagataagg atactggata tatacttata accccatcag gtataggaag
    17161 agaagaatta aacgttgaac atatttgtgt attggatttg aatggaaatg taatagaagt
    17221 tgagaaaggt attaagccaa gtagtgaggt attgatgcat attgaaatat ataaaataag
    17281 ggaaaatata aatgctatat cacatactca ttctttatat gcaacagcat tttcagtagc
    17341 aaagaaacca attataccaa ttgtatatga atcagttaat tatggcggtt atgtagatat
    17401 tgctccatac gaaagaccag gaactttaaa attagctaag agcgtttcaa aattactgat
    17461 agatacagat gcatgcttat tagaaagaca tgggttgaca actgtaggta atgatttaga
    17521 cgaagcatta ttaaaatcaa gatatgttga agaggtagca gaaatatact ataggtcttt
    17581 agtattaaat caatttaaag aacttgaagt agtagatgtt gatgagtttg aatcttggaa
    17641 atacccagaa gaaataaagt tttaatctaa ataattttca ttgaagtggt taattaaaat
    17701 atatgtatta tttatatcaa ttactatgtt ttattatatt aatcaccgta ttttgtaaat
    17761 atatttatta cattttttca aaaaaatata gaatccgaaa gccatataaa attgacttgg
    17821 gattctatac tttttattta tgcatttttc tttatctgat tttctggaga atttatatgt
    17881 tcaaaatatt ttttagtttc agatactatt ataggtgata atgcaagtaa agctataagg
    17941 ttaggtattg ccataagccc atttacaata tcagcaatta tccaaacaac ttctaacttt
    18001 aagaaagcac ctaataacac catagccaca aatacagttc tatacccatt catgcctttt
    18061 acaccaaaca gaaattcaaa acatctttct ccataataac tccatcccaa aattgtagta
    18121 aatgcaaata atgttaaact aactgtaagt aatattggtc caaatgtagg aattgctccg
    18181 ttaaaagctg cttgtgtcat aacagctcca tttaggtctg aacaccacac tcctgaaata
    18241 acaagagaga atccagtaag agtacagatt ataatagtat ctataaaagt acctgtcata
    18301 gatataagac cttgttccgc tggccattca gttttagcag cagctgcagc tattggagca
    18361 ctacctagac cagactcatt tgaaaataca ccacgagcaa taccatttct aattgccagt
    18421 gacatagttg ctcctaaaaa accaccagta gcagcagtag gtgtaaatgc acttttaatt
    18481 attaacataa aagtttcagg gattttattc caaaaagata ttaaaatgat tccacagatt
    18541 acaacatata taactgccat aaaaggtact attttagtag ctacattaga tatacttcta
    18601 agtcctccaa atacaaccaa agctacaaat actgctaata caacccctgt aattctaggg
    18661 tctatcccga aactattatt tattgcatct gttattgaat ttacttgagt aaaagtaccg
    18721 attcctaaaa gtgcaactag tataccactt atagcgaaaa atattgcaag aggtctccat
    18781 ttttctccca taccatttac tatgtaatac attggcccac cagacacttg accattttta
    18841 tctttagttc tgtattttat tgcaagaaca ccttctgaat acttagtagc cattccaaag
    18901 aatgctgcta tccacatcca aaaaagtgca ccaggtccac cagctttgat agctgtagca
    18961 acaccaacaa tattaccagt accaactgta gcagctagag cagtacataa ggctgcaaaa
    19021 cttgaaacat caccactacc tttgttttca gcagagaata ttaacttaag agctcttggc
    19081 agtttagtta tttgaacaac tccaagctta acagtaagta gtataccagt accaactaat
    19141 agtaccagta ggggtggtcc ccaaataaaa gcatcaactt tgtttaaaat atcaattaga
    19201 ttcataaaaa cactccctca ttttttttgc tttacataat caatagaatt tttaatgaag
    19261 taattgacag gaaataagta taatttatta ataatttcta gtgtaataaa aaaagctaaa
    19321 tctaaaaaag attcagctct ggatgtacac taattagcaa ccaaaataaa agtattagta
    19381 aatactgttt ttggtagata atccctgtcc ttttgcctga gagattgagc ataaaagcct
    19441 tgccccttcg gcgcccataa tgggtctctc cagagttccg taaattttac agtcctactt
    19501 ttaaaagtac ctgagagtgt tactccttcg gtgtgctata tagcaactct tctataaaat
    19561 cttaaccgga tattgatttg tcatagctta ttgtattaat aatattaata taagttttta
    19621 tgttttgtca actaaaaaat taaaaaacaa agaaaaaata taaaaatgta agcctattta
    19681 cgaattataa acttagaaag atgagtaata tacgtaaaaa tgaattttgt aatacacata
    19741 caaatgaagt gtaaaataag ttttaaataa aaaaataata aaatcttaaa taattaatta
    19801 gatatgatta aattatggta atattatagt tacgatatta agaaaagttc tatttagtta
    19861 gattttaagc gtaatatgag attatgtata gagaaaggat gtatttttag atgggaaaaa
    19921 tattagtgct agcagaaaaa ccttctgttg gaagagatat agcaaaagtt ttagggtcaa
    19981 aaaatgaaaa aaatggttat atagaggggc caaagtatgt gataacttgg gcactaggtc
    20041 atttggttac acttgctgac cccgaaagtt atggagaaag atataaatca tggagcttag
    20101 aagacctgcc aatacttccc aagcacctta agactgttgt aataaaaaag agtggaaagc
    20161 agtttaatac agttaaatcg caaatgaata gaaatgatat agatgaaata gttatagcaa
    20221 cagatgcagg tcgtgaagga gaacttgtag caaggtggat aatagaaaaa tcacaggtaa
    20281 aaaaacctat aaaacgtctt tggatttctt caagtacaga caaagcaata aaagacggat
    20341 ttgcaaaatt aagaagtgga aaagaatatg aaaatttgta ttactcagca atagcacgag
    20401 cagaagctga ttggattatt ggaataaatg caaccagagc tttgacaaca aagtacaatg
    20461 cacagctttc ttgtggaaga gtacaaactc ctactcttgc aatgttgctt aaaagagaag
    20521 aagaaataag aaatttcaaa cctaaagagt attatggatt agaattaatt gctacaaagg
    20581 gaaattcaga tataaaattt atttggaatg ataaaaacaa taattcttca acttttagta
    20641 aagaaaagat agaatccata ttaaaaaaag taaaaggtgt agacataaaa atatctgaag
    20701 taaacaagtc atataagaaa aaatattcac ctgcactcta tgatttaaca gaattacaaa
    20761 gagatgcaaa taaaatgttt ggattttcag caaaagaaac tttatctata atgcaaaaac
    20821 tgtatgaaca tcataaagta cttacttatc ctagaactga ttctagatat ttaacatcag
    20881 atattgttgg aacattaaaa gatagaataa aagctgtaaa tattagtgat tattcaaaga
    20941 tttgtaataa attattaaaa agcaatatta tagctaataa atcgtttgtt gacaattcaa
    21001 aggtaacaga ccaccatgca ataataccaa cagaacagaa ggtattcatg agtgatttta
    21061 gtgacaaaga gcgaaaaata tataatcttg tagtaaaaag atttttagca gtactatctc
    21121 ctccttttga atatgagcag acaactataa gtgcaactgt agaaggagaa atatttaagg
    21181 ctaagggaaa caaagtaata aatttaggtt ggaaagaaag ttataaagat attgaagatg
    21241 aagatgagta tgaaagtata ccaaatgttt tagaaaaaga tgtacttaag gtcaagggat
    21301 taaagataac ttcaggaaaa acaaatccac caccatactt aaatgaggga acattattga
    21361 ctgctatgga aaagaatggt ctagggacag ttgctacaag agcagatata atagaaaaat
    21421 tattcaattc atttttaatg gaaaagaaaa ataaagagat atatataaca tcaaaaggaa
    21481 gacaattatt agatttagtt ccagtagact taaagacacc agaacttaca gcttcatggg
    21541 aaaagaaatt attggatatt tcaaagggaa aacttagtaa aaatacattc ataaatgaca
    21601 tgaaagatta ttcaaaagat attgtactag aagtaaaaaa tagtgataat aaatttaaac
    21661 atgataactt aactaaaaat agatgtccag aatgtggtaa atttatgtta gaggtaaatg
    21721 gtaaaaaagg taagatgttg atttgtgaag atagagagtg taatactaga aaaactatat
    21781 cacaagtaac caattcaaga tgtcctaact gtcataaaaa attagaactt cgtggagaag
    21841 gtgaggggag aatcttcact tgtagttgcg gatacagaga aaagttgtct tctttcaaca
    21901 agagaaagca agaagaaaaa cagaaggcat caaaaaaaga tgttaatcaa tatttaaaaa
    21961 atcaaaataa aaatcaagag acatttaata atccatttgc agaagcatta gctaagctaa
    22021 aaaacaaata attttaaagt tattattata cacaattata actttaactg tatattagtt
    22081 atatgtactg atataatata gtgtatagtt taggcatgaa agtagggctg ttaaatggat
    22141 ttaagtggca tatttaaata ctattgtaaa gagtgtgaaa atacatggaa taattcgagt
    22201 gttgaattat ttgaggatat agaaacgtat agtaaagatt cacaaaaaaa gagggaaaaa
    22261 gaattagata aatttataaa tacaatatca gttcatttag agaggtatcc aagtgatgct
    22321 gtattgagaa aaatgtgggt aaaaaagggc gaggttttct tacaaaagac attggaaaaa
    22381 gaaaatattt ttaagttaga aaaaatggat gtagaggata gaaaaaaatt tttagatata
    22441 acaaaacagt ttattagaga tgctagaaaa tttgatgatg atttacctat aggtgatatt
    22501 atgcaagcta tgaggaatgt atggatttca aatgcattac aattattatt tggtaaagaa
    22561 atatattatt caaaagctaa ctttgcatat agtatgttat atccatatac tgataattat
    22621 ttagacaata caaatataga taaaaatgat aagattttat ttaataactg gttagaaaaa
    22681 aggctcctgg gagaacatat taaatctaag gattatcatg aaagtaaagt atctcaaatg
    22741 atagattata ttgaaagtgt ataccctaga gaaaagttta cacaagttta tgaatcgtta
    22801 ttattaatat ttaaaagtca agtaaatagt ttaaaacaac atggtaagga aaatcatttg
    22861 tgtaaagaag atttattatc catttctatt gaaaaaggag gttcatccgt tttagtagat
    22921 ggatatttaa taagtggatt tatgacaaag gaagaaatag agttttgtat aggatatgga
    22981 tttttattac aaatatctga tgatttacaa gatataaaag aagatttaaa atacaaccat
    23041 aaaactatta ttacagagat gtcaaaagag ggtactttag ataaagttgt aaataaacta
    23101 ataaatttta ctattgagtt aatagatagt tttaaaatta ataataaaaa taaatctgta
    23161 ataactatga taaagaatga ttgcttaatg ttaattttat tttctgtagt ttataatgct
    23221 gaattttttt ctgtaggata tataaaagaa gtagagaaat ttattccata tacaatagat
    23281 tattcattag agattgaaga aaaaataaaa gaaaaattta aaaatataga tgttttaaat
    23341 aatgaaaatg aatataaaga aatgattgat attatttgtg cagagtagaa aaaaagggag
    23401 tgtcttagaa tagacttttt agtttatgag gcactctttt tgtataaaat caaatgtatt
    23461 ttcatcattt taacaattaa aaagagctta tccctgtttg agaaagccct ttttaattta
    23521 aataaaaatg tcaaggttaa actatttaaa tgcagtaata atcccattat catgtaagtt
    23581 ttttatgatt gtagctgcga tagggaatcc aaataatcca gctattccaa atagttgagc
    23641 tccaataaac atacacatta atgttattaa aggatatagg ccaatttggt gtcctactac
    23701 ctttggttct aatatttgtc taacaacggt tataattatg tatagtatta aaagtcctat
    23761 tgctaaatta atatttccat taatgagtga gataatcatc caaggtgtca aaatacttcc
    23821 tgtacccaag actggcaaaa tatcaacaag tgctatgatg acagatatag taaatgcatt
    23881 ttcaaccttt aggatagtaa gtccaataag tagttctaag aatgtcactg atagtagtat
    23941 tgcataggct tttagtagtt ttaaaagagc attaatactg ctatttttta tatttattat
    24001 cataaattga cttttttctg gaaattgttt taagataaag tttactattt tttgatagtc
    24061 aaaagtaaag aaaaaagaag caattaaagt aataataagt tttaacataa atgcaggtgt
    24121 tttacttgca atactagcaa tagcattgag tgcatttgtt gaaatagaag ctactaaaga
    24181 catcatagat tgacttatat tatcccaacc aagtattaat gataggttaa cattaggtgt
    24241 actagcatct atctttgaaa ataaagtatt tagcgttggt tgtatacttg atttatatat
    24301 ttcaggaagt ctaaaaaata catcacttac acttgcaaat atcttcacac caaagctaac
    24361 taataaaaat atacacaacc cataaaaaat taaaagtatt actatagaaa taagtgtacg
    24421 atttaaattt gtattatttg taataagtct tataataggt cttaagatgg atgcaatgat
    24481 aaaactgatt acaaaaggca ttaataatgg caataagaat tttaatacaa tatatacaat
    24541 acttgttatt aaaactacat atgtaaattt aattataaaa ttcttttgct tttgaatatc
    24601 cataatataa aacctccaat gtatttgata ttattctaaa tatggtcttt tttacagaaa
    24661 atatactact gtatattgat tataaactat aaagcttatg taaagtaaat aaattatgta
    24721 aaatttaggt taattttgta taaagagata cttttatatt ttacaattaa taaccaaata
    24781 ctaaaaaata ttttaaataa agaacttaaa attgtgataa aattaattta tagatattaa
    24841 aaaaagtata atatatggtt tttaagaggt gctaagatga aaaattttag agatttaggt
    24901 gggaacaaaa ctgaggatgg cagaactgta aagaagggat tattttatag gtctgcaaaa
    24961 ttatcaaatc taagtgaaaa tgatataaag attttaaaag agttaaatat taaatatata
    25021 tttgattata gaagtgatga agaagctcgt aaacatccat caacaataat atcaaatata
    25081 aaaaatataa gaataccagc aatgagagag ctagaagaat caggaggaag ttttggttct
    25141 attgaagata tgattgatgg attgtttgaa aaagatggtg catttaatat gttaaataat
    25201 agttactata atttgcctat aaataatccg tcatataaaa aattagtaga acttataaga
    25261 gactattcta atttaccaat attaaatcat tgtacagctg gtaaggatag aactggtgtg
    25321 gggagtgcta taattcttat gattttaggg gtatcgagag agaatataat gaaagattat
    25381 ctaaaaagta atgattttgc agataaagag atagaaagat ttatagaata taagcctaaa
    25441 tttaaggaca ttccaaaaga aaatttaaaa tatatatttg gtgtaaatga agagtatatg
    25501 aaaactgcat ttagaagaat tgatgaagaa tacattagtg tagaagcata tttatatgga
    25561 gaatttaatt taaataaaga agaaataaga aaattaagaa atcaatatct agagtaaaag
    25621 gtgagtttat gagtttaaat ataacagcaa gacagataaa tataataaaa atattgttaa
    25681 attcaaaaga tacaattagt ggtattgcct taagtcaaga gataggatgt tcttcaaaaa
    25741 ctatacaaaa tgaaataaag gatattaata aacaacttag aaatggtaaa attttgtcaa
    25801 taagaggagt tggttataaa ctagaaggaa gttttgatga ggtaaatcta aatagcaatc
    25861 tctatgatga tgtagataga gtagaatata taatcagaaa aatattaacc ttaagtaatg
    25921 aagaaaaaaa tactataaaa cttgaagatt tagccgattc tatgtatgta agtgtttcaa
    25981 cagtaaaaaa tgatttaaaa gaagtaaaaa gaatattgga aaagtattca atatcagtta
    26041 aatctaagca taaacaaggt atatgtgttt tggaaagtga agataagata ttaaattgta
    26101 tagttgactt aagcaataag agagataatc aattgagttt aaatgatttt ttaaatgacg
    26161 atataaagga aagaaaacta ttaataaaaa aattattatt agacatatta aatgaaaatg
    26221 gattagtatt aactgatata gaatttaaga gtgtgttaaa taatatatta gttatgttaa
    26281 gcagacataa gtataatgaa aaagaattta tagagaatta tatagaaaat tataggagca
    26341 aaagaagctc aattttagaa aatgaacaaa acaaagaaat tataataaat tcaataaaaa
    26401 cattttgtaa aaatttaaaa ttagctacat caatagatat aagtaatgat aaggtatttg
    26461 aagagttttt atataagcat attagtagtt tatgtaaaaa aataaagtta ggtataaatc
    26521 aatcacctat aatggctcaa gatataaaaa taaaatatcc atttgctttt gaactagcta
    26581 atatagctaa aaagacaata gaaaaagatt tgaagataga aattaatgaa gatgaagttg
    26641 caaacatagc tctacatata ggaggagcag ttgaaagagc atcttataat aataaagata
    26701 aggttttcaa aactataata gtttgtactt caggcatagg cacatcaatg ttaataaaag
    26761 ctaaattaga gaatatattt aaggaaaaat tagaaatact aaagattatt ccttcatact
    26821 tagttgaata tataaaagtt atagatgttg attttgtaat atctactgtt cctattgaag
    26881 taggagatgt gccagtaata aatatatctc caattttaag tgagaaagaa attagattaa
    26941 ttgaaaaata tatagaaaca ggaaatatat acctagattt agatataaaa agcctgtttg
    27001 atagtgaatt attttttact gatttggtgt ttaaaaggaa agaagaagtt atagatttta
    27061 tggcaagtaa acttgtacaa aagggctata tagatgagga tatgaaaaaa tcttactttg
    27121 agagagaaaa aatagcaaca acagaaatag ggaatatggt aactatacca catggagcta
    27181 ttggaaaaat ttttgataat aaaatagcaa ttggtatatt aaagaagcct atttcttggg
    27241 aaatatcaga tgttcgttta ataataatgt tagcccttga taaagataaa atattagatt
    27301 atgaagtaat attttcaaaa atatataaaa gagtagattc tatagcaaaa gttataagta
    27361 tatgtgaaaa tagtagtttt gaaaaatttg taaatttatt taattagatg ggtagtattg
    27421 tatatacacc tgtaaatttg gataatataa aactaaataa gataaaaacg ataaatttct
    27481 cttttaatta agagaaattt tttgactttc aaggatatta atataaagtt aagatataaa
    27541 cataaagcaa ataaataaaa aagaggtggc aatatgattt atacagtgac attaaatcca
    27601 tcaatagatt atatagttaa gctagatgag ctaaagacag gaagtacaaa tcgtgtaaaa
    27661 gaagaatacg tttatccagg aggaaaaggt ataaatgtat ctcgcatact taaggaactt
    27721 ggaaatgata atacatcttt aggttttata agtggtttta ctggagaata tataataaga
    27781 acattggaag aaaaagaatt gaagacagat tttataaaga taaaaagtgg tttttcaaga
    27841 ataaatgtaa aaataaaggc gttaaaggaa actgagatta atggacaagg tccaaatata
    27901 gatgatgaag acattgatac tttatataaa aaacttgata agctaaatca agatgatatt
    27961 ttaatattgg caggaagtat accatctact ttagatgaaa aactatacga aaatataatg
    28021 gctagattag aaaaaaagaa tataaaagtt gtagtagatg caactaaaaa tcttcttctt
    28081 aatgttttaa aatacaaacc ttttctaatt aagcctaata acgacgaatt agaagaacta
    28141 tttggtgtta aattaaattc aattgaagac atggtaaaat atgctaaaag attaaaggaa
    28201 atgggagcta taaatgtctt ggtatcaatg ggaaaagatg gagcattgtt gattacagag
    28261 gaagatgaag tgcttataag tgatgtccct aaaggaaaag taaaaaattc tgtaggagca
    28321 ggtgattcaa tggtagcagg atttatatct gggtatttga atactgggaa atatgattat
    28381 gctcttaaat tgggagcagc ttcaggtagt gcaactgctt tttcatatga cttagccaaa
    28441 agagaataca ttgataaatt ggtaaatgag atatctgtaa agcagtttta aaatattaaa
    28501 aaatatatag aaaggtaatt aatatttatg aaaattatag atttattaga tgaaaagtca
    28561 attaagttaa atttaaattc aaaaaccaaa tcagaagcaa tagaagaatt agtagatttg
    28621 gtcgcaaata gtggaaattt aaatgataaa gaaaactaca aaaaagcaat tttggctcgt
    28681 gaagaaatga gtacaacagg aattggagaa ggtgtagcta tacctcatgc taaaaattct
    28741 tcagttacta aagcttgtat agcagcagca gtgtcaaaag aaggtataga ttatgaatcg
    28801 tttgacggaa gtttatcaaa tctatttttt atgatagctg caccagacgg agctaacaat
    28861 actcatcttg aagtattatc aagattatct acaatactta tggatgaaga ctttagaaat
    28921 aaacttataa attcaagctc tgaaaaagaa tttaaggaaa taatagataa aaaggaaaga
    28981 gaaaaattca gtgaagaata tcaagatgag aaagtggctg aaaaaaatat aatagatact
    29041 aaagagaatg atgctaataa gtatcctaag gttttagcag taacagcatg tccaactgga
    29101 atagcacata catttatggc agctgaaagt ttaaataaaa tggcagaaaa caaaggtgta
    29161 tcaataaaag tagaaactaa tggttcagca ggtgttaaaa ataagcttac aaaagatgaa
    29221 atagaaaatg caacatgtat aatagtggca gcagataaaa atgttgagat ggctagattt
    29281 aatggcaaaa aagtcataaa aactaaggtg gctgatggta tacacaaagc ggaagaactt
    29341 atagacaagg ctgtaaatgg agatgctcca atttaccatg gaggagatgg aagtcacaac
    29401 gaatcaaatg aagaaagtga aagtggattt agaaaagtat acaaacactt aatgaatggt
    29461 gtatctaata tgttaccatt tgttattggt ggagggatac ttatagcgat agccttttta
    29521 cttgatgatt atactattaa cccttcaaac tttggttcaa atacaccgat agcagcgttt
    29581 tttaaaggga taggagataa agcttttgga tttatgttac ctgtacttgc tggatatata
    29641 gcatactcta tatctgatag accagcgttt gttgtaggat ttgtaggagg tgctctagca
    29701 ggtgatggtg gttcaggatt tttaggtgca ctacttgcag gttttatagc aggttattta
    29761 gtagaagggc ttaagaaaat atttagtgta ctaccagctt cattggaagg aataaaacca
    29821 gtattgttat accctttact tggaacactt ttgatgggaa ttataatgac atttttagta
    29881 atacctccag ttacagcaat taataatgca atggttggat ttttaaatgg tttaggcgga
    29941 acttcaaaaa tagttttagg attagtatta ggtggaatga tggctgtgga tatgggtgga
    30001 cctgtaaata aagcagcata tgtatttgga gttgcatcac ttgaatcagg gcagtttgaa
    30061 ataatggcag cagtaatggc tggtggtatg gttccgccac ttgctatagc tcttgcaact
    30121 acatttttta agaatagatt tactaaagag gaaagagatt ctggtaaagt taattatgta
    30181 atgggattat catttgttac tgaaggagct ataccttttg cagctggaga cccacttcat
    30241 gttataccag cttgtgttgg aggttcagca gtggcaggag cacttagtat gttgttcaac
    30301 gcagctcttc gtgcacctca tggaggagta tttgttatac cagtggttac ccatccattt
    30361 gcatatatat tagctatagc tgtaggtgct ttggttggaa tgatgctttt agcattactt
    30421 aaaaaaccat taaatcaaga agtatagcaa ttaaaggata ttcaagaact taaaatatga
    30481 tgttttataa aaattaatat attgctgaat tattttaaaa taaggatttt attaaaataa
    30541 ggattttatt aaaataagga ttttatgagg ctgttatata acgatttgta attgttatct
    30601 tgacagtctt ttatttttat ataagtaaaa taagtgtgtt ttgctttatt tgtatttaaa
    30661 ttgtttttaa gttag
//
LOCUS       DAMKPG010000041        30667 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMKPG010000041 DAMKPG010000000
VERSION     DAMKPG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.044"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            122..2023
                     /locus_tag="PC621_001956"
     CDS             122..2023
                     /locus_tag="PC621_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4109115.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2026..3069
                     /gene="ypdE"
                     /locus_tag="PC621_001957"
     CDS             2026..3069
                     /gene="ypdE"
                     /locus_tag="PC621_001957"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF4109116.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3084..3389
                     /locus_tag="PC621_001958"
     CDS             3084..3389
                     /locus_tag="PC621_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4109117.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3486..3821
                     /locus_tag="PC621_001959"
     CDS             3486..3821
                     /locus_tag="PC621_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4109118.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3853..5175
                     /locus_tag="PC621_001960"
     CDS             3853..5175
                     /locus_tag="PC621_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4109119.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5185..6255
                     /locus_tag="PC621_001961"
     CDS             5185..6255
                     /locus_tag="PC621_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4109120.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6260..7447
                     /locus_tag="PC621_001962"
     CDS             6260..7447
                     /locus_tag="PC621_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4109121.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7644..8516)
                     /locus_tag="PC621_001963"
     CDS             complement(7644..8516)
                     /locus_tag="PC621_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF4109122.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8910..9101)
                     /locus_tag="PC621_001964"
     CDS             complement(8910..9101)
                     /locus_tag="PC621_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109123.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9321..10367
                     /gene="cobT"
                     /locus_tag="PC621_001965"
     CDS             9321..10367
                     /gene="cobT"
                     /locus_tag="PC621_001965"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF4109124.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10399..10959
                     /gene="cobU"
                     /locus_tag="PC621_001966"
     CDS             10399..10959
                     /gene="cobU"
                     /locus_tag="PC621_001966"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF4109125.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            10969..11742
                     /gene="cobS"
                     /locus_tag="PC621_001967"
     CDS             10969..11742
                     /gene="cobS"
                     /locus_tag="PC621_001967"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF4109126.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11762..12376
                     /locus_tag="PC621_001968"
     CDS             11762..12376
                     /locus_tag="PC621_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF4109127.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12443..12622
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12752..14251
                     /locus_tag="PC621_001969"
     CDS             12752..14251
                     /locus_tag="PC621_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF4109128.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14290..15666
                     /locus_tag="PC621_001970"
     CDS             14290..15666
                     /locus_tag="PC621_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF4109129.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15667..16638
                     /locus_tag="PC621_001971"
     CDS             15667..16638
                     /locus_tag="PC621_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF4109130.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            16670..17740
                     /locus_tag="PC621_001972"
     CDS             16670..17740
                     /locus_tag="PC621_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF4109131.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17742..18629
                     /locus_tag="PC621_001973"
     CDS             17742..18629
                     /locus_tag="PC621_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF4109132.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18617..19249
                     /locus_tag="PC621_001974"
     CDS             18617..19249
                     /locus_tag="PC621_001974"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF4109133.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19258..20484
                     /locus_tag="PC621_001975"
     CDS             19258..20484
                     /locus_tag="PC621_001975"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF4109134.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20481..21089
                     /locus_tag="PC621_001976"
     CDS             20481..21089
                     /locus_tag="PC621_001976"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF4109135.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21079..21654
                     /locus_tag="PC621_001977"
     CDS             21079..21654
                     /locus_tag="PC621_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF4109136.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21647..22399
                     /locus_tag="PC621_001978"
     CDS             21647..22399
                     /locus_tag="PC621_001978"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF4109137.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22383..23504
                     /gene="cbiG"
                     /locus_tag="PC621_001979"
     CDS             22383..23504
                     /gene="cbiG"
                     /locus_tag="PC621_001979"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF4109138.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23501..24226
                     /gene="cobJ"
                     /locus_tag="PC621_001980"
     CDS             23501..24226
                     /gene="cobJ"
                     /locus_tag="PC621_001980"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF4109139.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24223..24975
                     /gene="cobK"
                     /locus_tag="PC621_001981"
     CDS             24223..24975
                     /gene="cobK"
                     /locus_tag="PC621_001981"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF4109140.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            24994..26472
                     /locus_tag="PC621_001982"
     CDS             24994..26472
                     /locus_tag="PC621_001982"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF4109141.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26502..27407
                     /gene="hemC"
                     /locus_tag="PC621_001983"
     CDS             26502..27407
                     /gene="hemC"
                     /locus_tag="PC621_001983"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF4109142.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27397..28896
                     /gene="cobA"
                     /locus_tag="PC621_001984"
     CDS             27397..28896
                     /gene="cobA"
                     /locus_tag="PC621_001984"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF4109143.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28913..29878
                     /gene="hemB"
                     /locus_tag="PC621_001985"
     CDS             28913..29878
                     /gene="hemB"
                     /locus_tag="PC621_001985"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF4109144.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30031..30609
                     /locus_tag="PC621_001986"
     CDS             30031..30609
                     /locus_tag="PC621_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF4109145.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12413 a         3064 c         5669 g         9521 t
ORIGIN      
        1 tttggaatca aaatatacta ggatataaaa ttcttaaata aaaagaaaag gttgttttta
       61 taaaaaatat tttttatacc actttaaaaa acacaataat aacactgagg gaggtgggga
      121 tatgagagaa cgcattattc ttgaaacatt aaaaaatagt agtggttatc taaacataga
      181 ttattttgca gataagttgg gggtatctac tcgaactatc agaaatgaaa taaaaaagat
      241 agaaactata aaagaatgta atggttttaa attagaatat aagtctaaat taggatatat
      301 tctaaatatt aaagaccaag ataaattcga gcattatcta agaaacttac cctcagattt
      361 agttgaaaat ccagaacaac ggttggagtc tataatagta gagcttttag taaatgaagg
      421 ttataaaact atagaacagt taagtaaaaa atttctagta agttcatccc aaataaaaaa
      481 tgatttaaaa aagatagatg aaaagataaa ggatacagaa ttaaaactag agcgaaaggc
      541 tcattatgga attaaaatag aaggttctat taaagaaatt caaagtatat tggtggatag
      601 ttattttaga gggaatagaa atataacaga gtacaggaat aagtttatag ataatttaaa
      661 attagctaat attagaagta caataaaaaa tgtacttaat gaacatgatt tagaagcaaa
      721 tttaacagaa cttgaggaaa tattagcaca aattattata ttgtatataa gagttagtat
      781 gagagtagtg ggaaatgcta gcaatttaaa attatataca gaagatttaa ttatagataa
      841 attattagat aaaattttta gagataaaaa atataacctt aattatgatg aaaagtatta
      901 tttaaaggag cttataaaac taaagactaa agataagaaa gccactataa agaatattga
      961 taagaataaa cttcaagata ttatgttcga attttttaga gagatagata aaaaatataa
     1021 ctctaatttt ttagaagata aagagttttt taacttattt tacttacata ttgcatgttt
     1081 aatagaacgt ataaagaaga accacaagat aataaatcct ttttcagtta aaataagtca
     1141 acagtatcca actgttttta atttatctat tcaattttca aaaatcatag aaaatgagta
     1201 tcatataaaa ataagtcaag atgaaatagg gtttatagct actcacattg ctgttccttt
     1261 tgaaaagaga gaagaagcta attttaataa aaaatataaa atagcaataa tatgctcttc
     1321 tggtggaggt agtgcatttt tgataaattt aagacttaaa gaaatttttc caaatgctga
     1381 gataagaaat ttttcattac tagaagaaaa agctgtaata gaatttgcac cagatttgat
     1441 tttttcaata acaaatcttt tatttgaaat aaatgctcca gttatactta taaatgaaat
     1501 tttagacgaa ttggattatt tagatattaa agaaagtgtt agatttgcag accatatagg
     1561 aaatatatca agcccaaaac aatatatatt aggtctattt gataagaatc actttaggtg
     1621 tataaaagat aaagctaagt ataaggatat ccttgatagt atgtcaaaat gtatagttga
     1681 cgagggagct tgttctccaa cctatcccaa agatgtatgg gaaagagaaa gctatttgag
     1741 tactatttat acaaatgggg tggctatacc tcatccaatc gagatgacag gaaacaaaaa
     1801 tataatatca gtagctttga tacaaactga tattatttat gaaaataggg tgcccaaaat
     1861 tattttcatg atttcattaa taaagggaaa tttagaactt cataagcaaa tatctaaata
     1921 tttaagtaag attatggcaa ataaggatat ggtagacatg ctaaataagt cacaatctta
     1981 tgaagagttt atgtataagt taaaaatcta tctgggaggt tagacatgaa tatagagttt
     2041 tttaagaggt tgtcagaagc agatggtata gcatctaatg aagaagaggt tagacatgtt
     2101 ttattggaag aactaaaggc ttatagtgat aaaattatct gtgatgggct agggagtatc
     2161 atcttttcta aaataaaaga tgagagtgca ccaaacgtaa tgatttgtgc tcatatggat
     2221 gaagttggat ttatggtgag aagtatagac aaacttggta tgattcactt aataacaata
     2281 ggaggagtaa aacctcttgc acagtttgta caaaaagtta gaattacaac aaaagaagga
     2341 aagaaaatcc ctgctgtcat aaatgctaca tataataatg gtaaggctga aaatatatat
     2401 gcagatattg gagcatatac agaagaagat gtatataacc tagggattaa tgttggggat
     2461 atggtaacat atacaacaag ttttgaggaa tttactttac cagaccgatt agtgggaaag
     2521 gcttttgatg ataggatagg atgttttgtg atgggagaag ttttaaaaga acttaggaaa
     2581 gaaaatctaa attgtaatat tcactttgcg gctacaagta gtgaagaggt tggaataagg
     2641 ggagcaaaga catcaacaca gttaataaat ccagatatag tatttgtaat agatgtagca
     2701 tgtgctaaaa atgagtttgt gagagactat acaaatcaga aacaaattga taaaggtgta
     2761 atgctaatgc atagagatag gactctagtc cctagtagaa aaatgataga ttatgtaatg
     2821 gaaattgctg ataaaaataa tataccactt caacatgata tgtttgaatc aggtgggact
     2881 gatggaggag aagcacatct cgtaaatgaa gggaaacctt gtgtagtaac ttgtgtacca
     2941 gttagatatg gtcattgtgc attttcaata gtaagtaata aagatttaga gaacattata
     3001 agattgtata cacagttaat tttaaatttt gatgaagaac aatataaaca tataagaaat
     3061 tttatctaaa ggagaataag aaaatgtcaa cagaagtaca aataatggag cttatatcaa
     3121 ctgcaggaga gagtaaatcg aaagcttttg aggctttaaa aaaagtaaag tcaaaagatt
     3181 ttaaaggagc aaaattgtta atagaggagg ctcgtaaaat agatattgag gctcacagaa
     3241 tacagacaga attaataact caagatttaa atgaagatgt tgaaagccca acaatgagtc
     3301 ttttaatggt acatgctcaa gaccactata tgacttctca gctagcaaaa gacttgatag
     3361 aggctttgat atattgtctc gataagtaat tgtaatgtag aaatagatta tttatgtaaa
     3421 aattctttta aaagtttaga tgcttttaag agataataaa aatacaaact ttaggaggaa
     3481 ttattatgaa aatattatta tgttgtgcag gtggattttc tactaatatg ttaatgcaaa
     3541 atatgaaaaa ggtggtaaaa gagagtgcta aactcaatga aaaagatttt gagtttacag
     3601 ctataccagc agattcaata gaaagtgaag ttgatgattg ggatatagta cttataggtc
     3661 ctcaaatagc tcataaaata gacttcttaa aaggaatatt agagccaaaa aataagcctt
     3721 ttgcagtaat agataaagat gtatatggaa aaatggatgg agctacagtt atgaagttgg
     3781 cattaataac ttataagaaa catcaattag ctcaaggata ataaaaataa tgagggaaaa
     3841 gggagattag atatgttttc aaaatttgaa gcatttatga atagagtatt tatgccttta
     3901 gctcataagg ttgacaatca acgtcacctt ggttctataa aggctggtat ggttgctatg
     3961 acaccattca caatattagg aagtatattt gcaatacttc cagcacttcc aaatatgctt
     4021 ggagaaaata atccaataag caaattcata cttaacaatt cagcaatatt ggatttacca
     4081 gtaagtcttt ccataggttt aataggatta tatgcttgta tgtgtattgc ttttaatctg
     4141 gggaactact ataaattata tataccaggt tgtgttgcac tatcaacttt ttctttttta
     4201 ttcctagtag cctcatttac accagatgga gctttagaaa taggaaattt aggagctaaa
     4261 gggctattta caggtatgat agcagggctt ggttctgtgg agttatataa tttttgtaat
     4321 aagaagaata tcacaataaa aatgccagaa aatgtacctg attttgtatc taggtctttt
     4381 gaattaatac ctactactat aatagtttgt ggaactttta tagttgctcg ttttatttca
     4441 ttaaaagtat ttggagaatt acctccacag atacttactc aatttttatc tccattagta
     4501 ggaagtatgg ataacccatg gtcagttctt gcacttaact ttgctatatg tactatattc
     4561 ttttttggaa tacattcatc agtatttagt cctataacta gacctataat gattacattt
     4621 atagctgaaa acatagcagc tatgcaagct ggagaaacaa tacctcattt ctttacagca
     4681 ggagcttcaa gtgcattctt tggatttaca ggatgtggaa ttagtatagg ttgtgtaatt
     4741 gcatgtatgt tatcaaaaaa tgcttcctat aagaagattg gacgtgtatc attattccct
     4801 gcattatttg ggataaatga accaatatta tttggagcac cgataatatt aaatccaata
     4861 atgtttatac cacatgtatt tggtggagct ataataggta cactaccaat gttttttatg
     4921 cactggggat ggttagcaaa gccagtattt gaccctccat atgtaggggt attcttagaa
     4981 ggatttttaa ctaatggaga ttggcattct atattagcaa atgcattgca attagtatta
     5041 gcagttttat tatattggcc attctttaaa ataatggaac gtcaacaaaa taataaaaaa
     5101 caaaaagaag aaactaagaa tatattctct aaagaagaag aagacttatt ggatgattta
     5161 ggattggatt tctaatattt tgttatgaat aatacaatag agagattagt atccttaatc
     5221 tataaaagag gtgttgacgc tcttttgatt aaagcaaaaa atactaaaag atatatagga
     5281 gctttatcag gtagtggtgt atatgtgctt gttactaagg ataaaaatta tcaaattcta
     5341 gatggcagat atattgatga agcagataaa aaaacgactg gtttcataaa aaggattgta
     5401 tctcaaggtt cttatatacc aactataatt gagttattaa aagaattaaa tataaaaaag
     5461 ttagcaatag aaaaccaagc tatgagtata caagagtatc tttcactaaa aaatgaaggt
     5521 tttgaaatca atttaataac aaatgaatta tggaagctaa gggctataaa atctaaagaa
     5581 gaaattgaac ttattaaaaa ggcttgtgag attacagatg aagtatttga tgaggttatt
     5641 tctgaaatta aaaaagatat gactgagcta gaagtatcag ctctaattca gtatcatgca
     5701 ttaaaaaaag gtgcaagtgg aatgtctttt gaaacaatag tggtatcagg agaaagaggt
     5761 gcaatgccac atggaagacc tacaaacaaa aaattgaaag tgaatgaagc tataacaata
     5821 gattttggtg ttgtttatca agggtatcaa tcagacatga ctcgtacaat atctataggt
     5881 aaacctccta aaattattaa agagatatat gatgtagtat tggaagcaca attatctgca
     5941 atagagtcaa taaaggaagg aactagggca agtgatgttg acaaagtggc tagaaaaatt
     6001 atagataaac atggatttgg cgaatatttt aatcatggac tgggtcatgg tataggtctt
     6061 ggagatggtg aagttcccac tttaaatcca aatagtgaag atatattagt ggaaggaatg
     6121 gtaatgtctt gtgagccagg tatttatatt ccaaatgtag ggggagttag aatagaagat
     6181 gatattgtta taatagatgg aaaaggtgtt ccactcaata agacatccaa agagtttata
     6241 atattaggag agtaaaatta tgaaatataa ttttaataaa gtattaaatc gtaaagaagg
     6301 aaattgtaga aaatggagca accatgtgat taaagaaaaa ttcggcttaa atgaagatgc
     6361 aattcctatg gatttagctg atatcgattt tgagtgtgct cctgctataa aagaatctat
     6421 cttaaaaaga gcatcagtag gagattatag ttatactttt ataggagatg acttctatga
     6481 tgctattata aattggaata aaagaagatt tgatgtatat atagaaaagg attggataaa
     6541 attaactttt ggtactgttt caacccttca ttatatagtt caagcatata ctaaagaagg
     6601 agagggtgta ttgataaata cacctgctta tgaccctttt gcagaggctg tagcaaataa
     6661 taatagaaag ttgtgttgta gctcattaaa attagataac aatagatatt atttagattt
     6721 agaggatatg gaaaatcaaa tgaaatacga gaatataaga ttacttatat tctgtagtcc
     6781 acaaaatcca tcaggtcgta tatggacaaa agaagaacta tatcaagtgt cagagttatg
     6841 tttaaagtat aatgtaattt tggtaagtga tgaaattcat agagatataa tatttaaaga
     6901 ttatagattt gtatctttgt ggaatgctca tccagaaatt tataaaaata gtataatctg
     6961 cgtatctcct aataaaggct tcaacttagg tggattaaaa acatcatatg tattaataag
     7021 agatgtaaaa attagggaga ctttattaga aaggctaaaa agtaattcaa taacttctcc
     7081 aaatgtattt gcaataccag caatagttgc agcatacaat gaaagcgaag aatggctaga
     7141 tgctatgact agttatgtag aggagaattt tgaaatagtt tatgatttct ttgaaactaa
     7201 tataccaaaa gccaaagtga tgaagtcaga ctcttctttt ttagcctgga tagatgtaag
     7261 agaagtattt agaaatgaag aagaatctaa agaatttttt agacatgcaa acttaactat
     7321 ggtagttgga agttattttg tgaaagatgg agatggattt attcgtatta atgtggggtg
     7381 tcctagagaa acattaaatg aggctctaaa tagaatcaaa aaaacgtata taagtatgta
     7441 ttgttaaatc ataaaaaaag tatctatggt ttatataaaa acacattatt tagttatcaa
     7501 atattactgg aattttgtat tccagtaata ttttttgatt aaggaatatt attgaggaat
     7561 attaaataat attatttcat acccttatat taacttatat aaaaatattt tattattttg
     7621 ggaacgggtt agctaacctt tatctagtgt atatttatat tgtaagaagc agtaaaaact
     7681 tttcccttac taagtttatt aataaacttc ttatctttat aaattttgtt agaatttata
     7741 ctatcagcaa gaccatacca aggttctata cataaaaaag gagcagtaga attagtttca
     7801 ctataataag gagtccaaat accaactaga gggaaatctt ccatagatat agttacatat
     7861 ttactaccat cattattata tatagacact tcatctatgt tggtgtatat caaagcatca
     7921 ttcttgaata cttctggatt taaatgtaag ttttttaatt ttccaatagg tattatatca
     7981 tctgtaaaag aaccatttaa attgattttt tctacatcat ttttactttt aaactcaagg
     8041 tagtgtttag aaaaatcatt ttgtttgtaa aaaggaaggt tgaatgcagg atgtccacct
     8101 atagaaaaga atatatcttt agagtcagta ttttttacaa tccattctac ctcaatacta
     8161 ttatcattta atgtatagtt tatgattagc tcaaaagaat atggatactt ctttaaagtg
     8221 gattcattat ctgttaattt ataagataca aatgtatctc ctttatctac aatctcaaag
     8281 tccatatctc tagcaaaccc atgttgagtc atgctatata aattttcttc tattattgtt
     8341 tcattatcaa aaagtttacc tactataggg aacaatatag gtgattgtct accccaatat
     8401 tttttattac catcccaaag aatgtttttg tcgtattttt tagaaaatat tttagttagt
     8461 tcagcaccaa aactgcttga ttcgataatt aaattttcat tttgtaatat attcatttaa
     8521 aatctccttt attatatttt atgagtatat tatactataa agtataaaga ttattaaatt
     8581 ttatttgata ttatatttga aaggtagatt ttagtatatt ttgaaaattt tgttttgaaa
     8641 atttgggatt atttaagaaa ttttgtatta taaatagaag gaaatagata aattgtatag
     8701 aataaaatat attaagataa gttaataatg ttaaatggga agtgagttta tggaaaaacc
     8761 aaatacaaaa gttgttaact ttcaagagta taaagacaga aaaaaccaaa gagaagtaaa
     8821 tagagacgaa cttttaaagc ttataaaaaa aattgtgact tcaaaatagg gagttgttaa
     8881 actccctatt ttaatgggtt tcataataat taagacattc tgagtccatc tatataatct
     8941 ttgaattgtg agaaatctga ttcactactt aattctatgg tttctgaacc ttccgtataa
     9001 aaattcttaa gatagatagt ataattatta tcaacacaat catgataaat acatctatat
     9061 ccatcttcat acaaactttt taacttagaa aaatcatcca caatatcatc tccttaaagt
     9121 tcttttaaat atatttttac aaaaagcatc taattgtatg caaattgaca atgtaaaata
     9181 aaggaaatga acataaaatg tagaataatt tttatatgta tgtaaaaata aaaagaaaaa
     9241 gttgaaattt ttagaaaata agagtatttt ttatctaaaa agagagttta aaaaataaaa
     9301 aaatgttggg aggataaaaa atgagtttat tagaaagtat atcaaaaaac atatattccc
     9361 tagataactc ttctattgaa aaaacaaagc agagattgga taggcttata catccaactg
     9421 gaagcttagg aaagatagaa gatatttgca tgcaattggc aggaatattt ggcaatgaaa
     9481 attttgacac aagtaaaaag gttataattg cttttgcggg agaccatggt gtatatgaag
     9541 aaggcgtagc acctgaccct caaaatataa caaaattaca gtttccaaat ttttcgaaag
     9601 gtctatgtgg agttggagta ataagtaaat ttgtaggggc agatgttgta gctgtagatg
     9661 taggaataaa ctgtgatgaa aaactggatg gggtattaga ctacaagata agaaaaggga
     9721 catcaaatat ggcaaaaggt ccagctatga gtaaacaaga agccattaga tgtttagaga
     9781 taggtattga aatagcagag cagtgtatag aaaaagatta taaggttatt ggtattgggg
     9841 agatgggtat agcaaatact actccaagta cagcaataat atctgtaatt tcagggtgtg
     9901 acccgttaga agttactggt ataggtgcag gtcttaagaa agaaagacta aagcataagg
     9961 ctgaagtaat tagaaaggct attgaaatta ataatccaaa tccaactgat ggagtagata
    10021 ttttatctaa agtaggagga tttgaaatag gctcaatggc aggtgttata ttaggttgta
    10081 gtgctaatag gattccagta gtaattgatg ggtttatttc ttatgcagca gcattaattg
    10141 catataagat aaatccaaaa actagagaat atatgatagc atctcattta tcagcagaat
    10201 caggaactaa aagagcatta gacatattaa aattagaccc tctattaaat atggatatga
    10261 gattaggtga gggaagtgga gctgctttag catttgatat aatagaagct tcaaactata
    10321 cttacaaaaa tatggcaact tttgatgaaa ttgacatggg aagataaata taaaaattta
    10381 atatgtagga gtgttttgat gagtaaaatt attctagtaa caggaggagc taggtcaggg
    10441 aagagtaact ttgcagaaaa actatgtttt gacagaaata ataatacagc ttatatagca
    10501 acctctattc catttgatga tgaaatgaaa gatagagtaa aaaaacatca agagaataga
    10561 cctagaaact ggaatactta tgaaatatat gaagatatat attccataat taaggagata
    10621 tataaagaac atgaaactgt tatactagat tgtgtaacat tattagtaaa taatctaatg
    10681 tttgaacaca atctaaatat agaagagtct agtcaagaag aaataaacaa attagaacaa
    10741 tacataaaag aacaagttgt caaacttatt gaagaaatag aaaaaacaaa tctatacttt
    10801 gtatttgtaa cgaatgagtt aggtatggga attgttcctg ggaataaact tagtagaatt
    10861 tatgctgata ttgtaggtcg tataaatcaa tatatagctt cgaaaagtaa tgaagtttat
    10921 tttgtagtta gtggcatagc tatgaagata aaggagtagt atgcaattat gaaaagattt
    10981 attttaatac tacagttttt gacaaggata cctataaaat taaatgtagg atttgatgat
    11041 gaattttata agtcaatagt atattttcca cttgtaggtt ttgtcatagg tatattatcc
    11101 tatttaatag gatggatttc tatgctttta tttgaaccat ttatagcttc tataataata
    11161 actctagctg gtgtcttaat tactggagga cttcatatag atggattagg agatacattt
    11221 gatgcaatat atagttatag ggataaagaa aaaatgcttg aaataatgaa agattcaaga
    11281 ttaggtacaa attcacttct agcaattatg tttgtacttt tattaaaggt tggattcgtt
    11341 tacgatataa taagtaataa ttcattgtgg attataattt ttatgccaat gattgcaagg
    11401 ttaggagtaa tgttattgac atataaaacc gtgacaccaa gagaaaaagg aatgggaaat
    11461 ttatttatag gaaaattaac tacaagtatg ttaataactg ctataatata cacattattg
    11521 atagttgctc ttattactaa atttatattt ttattaccta atatagtgtt gattaaagta
    11581 ttaggttcga ttatagtggt ttttgtattt ataatattat tcaaaaaaca tatttataaa
    11641 aaaattgacg gagtaacagg agatatatta ggctgtggaa tagaactttc agagcttgta
    11701 tatttaattt acatatattt gttaattttt atgttttttt aaagtaaaaa gtggtgataa
    11761 tatgataaga ttaatattaa taagacatgc actgacaaat gataataaaa aaggaagatt
    11821 atcaggccat ataaatagtt gtattagtga agagggcaaa ctacaaataa ataaaattac
    11881 cagatatctg agtaatgaaa acatagataa ggtttataca acaccatcta cacgtactaa
    11941 agatacagta gaaaaaatat caaaattgaa attattagaa atagaagaaa aagaagcttt
    12001 aagagaaatt agttttggag actttgaagg aagaactttt gaagaaatta aaatcaaata
    12061 tccaaatgag tttgaaaaaa tgataaaaga aggtaacaac tatagatatc caaatggaga
    12121 aagtttgata gactcttata aaagagtggc aaaagaaatt gataatataa ttttagaaaa
    12181 tgatagtaat ttagatacaa agacaatact tatatgttct catgcaggaa caataagaaa
    12241 tataataaca catttgattt ctggtagtta taagtatcac tggaacttta aaattgataa
    12301 tgcatctatt acagttttag aaattgatgg tggatttgct gtaatagata aaatgaattt
    12361 tacagacttc atataattta atcaaaacca aacattacaa tctgtgtttg taatatatct
    12421 gtaaaattag caatgaaaat agaatattag attaaaatta ggttcttaat tagatgttaa
    12481 aagggaaaaa ggttagatgc ctttgcagcc cccgctactg tgaaaccaac gaaactataa
    12541 ataccactgt cagtttgatg ggaaggttat agaagtagga tgaagttaag ccaggatacc
    12601 tgcctaattt aatttaaaac aagaggtctt cggggtgaaa gatttttaat taatatatat
    12661 aaaagtccta tctttagaag tattaggact ttttttaatt tgttttttag taaattttaa
    12721 caaggaggga tggaaagtgg ggaaaaaaat aatgctacaa ggaacagctt caaatgtagg
    12781 taaaagtgtc ttagtagcag gtttgtgtag aatatttaag caagatggtt atactgttgc
    12841 tccttttaag tcacaaaata tggctttaaa ttcttttata actaaagaag gattagaaat
    12901 gggaagagca caagtatttc aagcagaggc tgcgaaaata gagccaatag ctgatatgaa
    12961 tccagtttta ttaaaacctt ctgggaataa taaaagtcaa gttattgtaa gaggtaaagt
    13021 tgtaggagaa atgccttctt ctaagtatca tgattataaa ttggagttag taggtgtgtt
    13081 gaaggaaaca tttaatagct taaattcaag gtatgatatt gtagttatgg aaggggcagg
    13141 aagtaatgca gagataaact taaaagatag agatatctct aatatgggta tggcagaaat
    13201 agctgatgca cctgtaataa tagtcggaga tattgataga ggaggcgtat ttgcctcatt
    13261 agcaggaaca atgcttttat taaatgaaga tgagaaaaga agagtaaaag gtgtaataat
    13321 caataaattc agaggaaaaa aagagctttt acaagatggt gtaaagatgc tagaagacat
    13381 cataaaagta cctgttatag gagtagtacc ttatacagat ataaaaatag aagatgaaga
    13441 cagtgtaact acgagattta agaaaaaaat gaataaaagt gatatacaca tagaaataat
    13501 tagaacccca catatgtcaa attttacaga ttttaatata tttgagacac aagaagatgt
    13561 aagtattaga tatgtagact atggagaatc tttaggaaat ccagatatat taataatacc
    13621 tggaactaaa agtactatag acgatttaac atatataaga aaaagtggtc ttgaatcgca
    13681 aatcaaaaat ttacatagtc aaggtaaatt gatatttggg atatgtggtg gataccaaat
    13741 gttaggtaaa aaattaaaag acccttatca tgttgaaagt gaaattgaag aagttgatgg
    13801 aataggtctt ttagatacag aaacaacgtt tgagaaagaa aagactacaa cacaagtaga
    13861 ggctactatt gttgaatgta tcagtgaata catgaataac cttaagggaa aaaaagtaaa
    13921 aggttatgaa attcatatgg gcattacaaa gcgaggtaat gatgtaagaa gtttagattt
    13981 aataactaaa aaacttgata aagaagtgaa ttatactgaa ggaagtgtaa ataaagaggg
    14041 gaatgtaatt gggacttatc ttcatggaat atttgatgaa ataagcttta caagagcaat
    14101 tttaaacaat ataagaaaga aaaaaggttt aggagaatta gaaagcaaag taaactcttt
    14161 taatgaattt aaggataaag agtatgataa gttggctgat tttttaagag aacatttaga
    14221 tatagagaaa atatatgaga ttatgaaata atgatgacct ttatttataa tttaggtagg
    14281 aggtgtatta tgaaaaaaat tttaattgct ggtactaata gtggagtagg taaaacaaca
    14341 atatcattag gaataatgca agctttggtt aagagaaata tgaaagtaca gccatataaa
    14401 gtagggccag actatataga cccttcatat cacactttta taactggaag acattctaga
    14461 aatttagatt cttatatgtt agatgatgaa aaaataaagt atatagtaaa taagtcctca
    14521 aaagatgcag atatttcagt aatagaaggt gttatgggct tatatgatgg ttttggaata
    14581 gatttagata attgtacaag ttcacatact tcaaaggtat taaagtcacc tgttatatta
    14641 gttataaatg gtaaatctat ggcagcatct agtgctgcta tggtacttgg atataaggaa
    14701 ttagataaag atgtaaatat aaagggtgtt atagtaaata atgtaaagac taatagccat
    14761 tatcaaataa ttaagagttc tatagaaaaa tattgtaatg ttgaagtatt ggggtatttt
    14821 cctccaaata acaaattctc tttagaatct cgacatctag gattagttcc tagtgtagaa
    14881 atgaaatcac taagggaaaa attttatacc ttagcagatg aaattgaaca ctatatagat
    14941 attgacagaa taattgaaat atcagaaagc gatgaatttg agagtgattt tgattttcaa
    15001 actttgatag aaaataataa aataaaagaa catgttaata ataaatcaat agctatagca
    15061 tatgataaag cattcaattt ttattataga gaaaatatag agcttttaga ggaacttggt
    15121 ttagaaataa actattttag ccctcttgaa gatgcatcag tgccaaatgc agattatatt
    15181 tatattggag gaggttttcc agaaatattt ggaaaagaat tagaagcaaa tgagtcaatg
    15241 agaacttcca tattagaagc acataacaat aatataccta tttatgcaga atgtggagga
    15301 ctgatgtacc taggtgagaa actattgaat caagaagaac aagaatttaa tatggttgga
    15361 atatttagtg gaattagtaa gatgacatct tctttaaaga gatttgggta ctgtattggt
    15421 gtagccaagg aaaatactct tttatctaaa aaaggggaat ctattaaagg gcatgagttt
    15481 catcattcaa tatttgaaag tgatgaaaaa tgtgtatatt caatgagaaa agaaagagat
    15541 ggaaatatag tagaagagtg ggatggtggt tatagtaaag gaaacacttt ggctacatat
    15601 cttcatactc atttttataa taacttaaat tgtatagaaa attttttgaa aaaaggaaat
    15661 gaataaatga gtattttatc tatttatata ggatatgtga cagacttaat tgtaggagac
    15721 ccatattctt ttcctcatcc agtgagattt attggtaaac ttataaattt tactcaaggt
    15781 ataataagaa aggtctctaa aaatgatagg cagttaaaat ttgggggatt tatattatgg
    15841 tttataaccg ttggaataac atatttaaca acttatgcta tagtgaagct atttagtttt
    15901 aatatattat tgagtattgt agtgaatagt tttataattt atacaacact agccacaaaa
    15961 tgtttaaaag acgaagcatt aaaaatttat aatgtattaa aaaccgaaga tatagaaaaa
    16021 tctaggattc agctatctta tatagtaggt agggatacta ctaatttaag tgaacctgaa
    16081 ataataagag caactgtaga aactgttgca gaaaatactg tagatggtat aattgcgcct
    16141 atgttttatg catttattgg aggagcacca cttgctatgg cctataaagc tataaataca
    16201 ttagactcaa cagttggata taaaaataaa aagtataaag atattggttt tgcatctgct
    16261 aagattgatg atatagccaa ttatatacca gctagaatat ctgttattct tatgacaatt
    16321 ggaagtttct ttttaaatta taactatagg aactgtttta aaatctctat tagagataga
    16381 aagaatcata aaagtccaaa ttgtgctttt tcagaaggcg cagtatctgg tgctctagga
    16441 atacaactag gaggtaccaa cgtttatttt ggggaaaaag tatataagcc aacaataggg
    16501 gataagctta gggagataga taaagaagat ataattaaaa ctaataaaat tatgtatgta
    16561 tcttcatttg tatctatgtt ggtatttaca ctaatacatg tgttatgtag gatgatttta
    16621 attaacttaa ttatataaat tattgtttgc taacttaagg gggacataga tgaaggattt
    16681 agggcatggt gccaatgtag gtgaaatggc aagattatat gggaaaaacc caaaagaaat
    16741 aattgatttt agttcaaaca taaatccaaa tgtattacca aatttagaga gatacatttt
    16801 aaaaggatta gaagagtgta ggaattatcc agatataaat tatacaaact tgagagaaaa
    16861 tatatcaaaa tatataggta taaatccaga ctttataata cctggaaatg gtgcaacaga
    16921 agtgatttat ttgcttatga agagtgttaa aaaaaaattg gctataataa atccgacttt
    16981 ttcagagtat agaagaagtg ctgagttaaa taatttagat attatagatt tagagctaga
    17041 tttagaaaat aactttaaac ttaatataga tataataaaa gaaaatataa aaagatttga
    17101 tagtttattt atatgtaatc ctaataatcc tagtggtaat gtccaagatt taaaggaact
    17161 tgttcattta ctaggtaaac ataataaggt actaataatt gatgaaactt tcatggaatt
    17221 tgttgaagat gaaagtaaat acagtttggt taaatatata gaatcaaata aaaatatatt
    17281 tataattaaa gctgtgacta agttttttgg tatgcctggt ttaaggctag gatatggtct
    17341 tacaagcaac actgaaatta tgaacaaaat ttatgaacac aaagagccat ggactattaa
    17401 ttcttttgca gatatactat ccaattttat ttttgaagat aaagagtata ttaaaaatag
    17461 taaagaatat tacatagaag aaagaaaata tatgttacaa gagttgagaa acataagaaa
    17521 tataaaggtc tatgatacag atgctaattt tattttgata agagcatata aaaaaacaac
    17581 taaagaattg aaaaaagatt tgcttaagca aggaaacata ttagttagag atgcgtctaa
    17641 ttttatagga ttagatgata gttttataag agtggctata aaatctcatg aagataacaa
    17701 aattcttata gaaaatatga aaaatttatt gggtgattaa aatgaaatct tatggaatat
    17761 gcccagcatc ttgtggagaa tttgtacaag gtataattga tgatgaagaa tatttatgtt
    17821 catatgcaat agatatgtat tctgaagtgt atattgaaga aaaattagta gatataaatt
    17881 tagggagata taagtcaagg ttagcaattg aaaaggtatt tgaaaaattt aatttaccta
    17941 aaaaatatac taaaaatatt tctttaaata taaatagtaa aatacctgtt ggaaaaggaa
    18001 tggcaagttc aactgctgat ataggagcta caataaaggc aacattatca ttgatagata
    18061 aagatttaag tagtgaggag atttctaaat tagcagcaga aatagaacca acagattcta
    18121 tatttataga taaaaatagt atatttaatc ctctaaatgg aactgttata aaatacctag
    18181 gaaatctgac caatgcgaaa gttataatac ttgaaccaaa caaagtacta gatactatga
    18241 aaattagatt gaggcaagat tataataaat taaaagtaga aaataaagaa gtaataaaaa
    18301 aatcatttgc tcttttagaa gaaggattga aaaaaaataa tttatctcta gttggagagg
    18361 cttgtacttt gagtagctta gcaaatgaga atatagaaaa aaaagagtat ttaaatgaaa
    18421 taataaaaat atcaaaaaaa tatggagcat atggagtaaa tattgctcac agtggaactg
    18481 tagtaggtat tttaattgat aagtctatga atgataaaaa gatgatagat accttatgtg
    18541 aatctaacat aaattctgtc tataataaaa tttatacaca aaacataatt aatggaggaa
    18601 ttaaggggga aatagaatgg aatacataaa gaatcctatg aaaatagaag aaaaaagttt
    18661 tgaattgata caagaaataa tagacgaaat tagaccagat tataaattta agaataatat
    18721 tgaagaaaaa ataataaaaa gagctataca tactactgct gattttgatt acttagatat
    18781 attaaaaata tcagaagaag cagttgatag tataatagat gcattaaaaa ataatgcaag
    18841 tatttatact gatacaaata tggctcttag tggtataaat aaaagaaagt tagaatcatt
    18901 aggatgtaag tataaatgtt tagtggcaga tgatgaaact gtaaaattag ctaaagaaaa
    18961 aggaattact cgttctatgg cagcagtaga aattgcagct aaagaagaag gaagaaaaat
    19021 attcgtttta ggtaatgctc caacagcttt atataaagtt atggagatga aatcagaagg
    19081 tagattagat ttagatgcag ttataggagt tcctgtaggt tttgtaggag cacaagaatc
    19141 aaaagatgaa gttgaaaaaa cagatatacc atatataatt tctaaaggta gaaaaggtgg
    19201 cagtaattta gcagcagcaa tagtaaatgc tatattatat actatgtagg tacctatatg
    19261 gaagaatatg tatatataga tggaaaaaaa tatagaagag gatatactac aggttcatgt
    19321 gcaacaggtg catctaaggc tgctgtatat atgttgatta caaaaaatag aatcaatact
    19381 ataaatatag atactcctaa ggggatacca ttactactaa aagttgataa tataaatatt
    19441 tctgatacat ttgtggaatg ttccataaaa aaagatggtg gagatgatat agatgcaact
    19501 cataccatgg atatttatgc aagagctgaa attgtagcta aaaatgataa gaacaaaggg
    19561 tatcttactc taaaggatat agatagttta agcaccaata gtgagtgtaa aagtgagctc
    19621 tataagttta ttagggttta tggcggaact ggtattggtg tggttacgaa aaaagggctt
    19681 agtgtggatg ttggtaaacc tgctataaat ccgactccac ttaaaatgat aaaccatgaa
    19741 ataagaaagc ttataggaga taattttgaa tctatacttg gaaatgataa agttttaaaa
    19801 ataacaatct ttgctccaca aggagaaact gtagcaaaaa aaacatttaa tccaagatta
    19861 ggtatagttg gaggaatatc tataataggt acaactggga ttgttgaacc tatgagtgat
    19921 gagggttgga agaagtctct ttctatagaa cttcagatga aaaaagaaca aggcttagat
    19981 aaaataatat tggttccagg aaatcatgga gaacagttta taagagaaaa attaaactta
    20041 gatattaagt atgtagttag ggtaagtaat tttataggct atatgattaa agaagctcaa
    20101 agaataggat ataaaaaaat cttaatggca ggtcatatag gtaaatttat aaaagtatct
    20161 gctggtattt ttaatactca tagtaaggta gcagatgcta gaagtgagat attagtggca
    20221 aacttagctt taatgggagc tagatacgaa tttttaaata aaataaatca atgcgtaact
    20281 actgaagaag ctgtagagct aataaataat agtgaatata gagaagtata taatatatta
    20341 agtaataaat gtaaggagag agtaaagcaa tacttgaatg aagattcaga tgatattgat
    20401 gtggaagtaa tcatattttc aatggataag tctttattag gtaaatcgga taatacagat
    20461 gatttagtgg aggtctttat atgataaaca taataggtct aggacctggt aacttagact
    20521 atataacaaa aaaaggtgaa aatttaattt ccacttctga tgtattaatt ggtggtaaaa
    20581 gaaatctaga gtctataaaa aattttgaag gagaaaaaat agttctggat tctaatttaa
    20641 gagaaattat agagtacata aataacaata aagaaaagca aatatcaata atagcatctg
    20701 gggacccatt aatatatggg ataggtagat atctatctaa aaatattgat aataagatgt
    20761 taaacgtggt ttctgggatt agttcattac agtacatatt ttcaaaaata tatgtagata
    20821 tgaatgatgt atatattaca agtagtcatg gcaaaatacc agattttgat tacatattat
    20881 cacataaaaa agtatgtatg gttacagatt caaagatagg accaaaacaa ataagtagag
    20941 aaataataga tagaaatcta aataaaatta ttgttgtagg agaaaatctg tcatatgaca
    21001 atgaaaaaat aacaatagcg aaaccagaag agataataag aatggacaat tttgatatga
    21061 atgttgtggt gattttagat gaggaatagt gaatttataa caggtaaagt tccaattact
    21121 aaggaagagg ttagagcaat atcaataagt aagttaaatt tagtaaatgc taaaaacctc
    21181 atagacatag gggctggtac aggaagtgta agtgttgaag ctgcatataa ttatcctaat
    21241 ttaaaggtta tatctataga gaaaaatgat gacgctatag agcttataga gaaaaatata
    21301 aaaaaattca acttaaaaaa tatagaagtg ataaaaggat atgctccaat taatctaggg
    21361 ttaaatgtta aagttgagtc tatttttctt gggggaacag gaaataatct ggaagaaata
    21421 atagaatggt ctaaaaaaaa tctcgttaca ggaggaagac ttgtagctaa ttttataata
    21481 atagatacat ttaatcaaac tttaaaactg cttaaaaagc atggatttaa agaaatagat
    21541 gtatgtgttt tgaatgtttc taaactagag aaactaggaa aaggtgaata ttttaaacca
    21601 ctaaatccta tatatataat atcttgtgaa aaaggggaaa atgatgatga ataaagtaca
    21661 ttttgtagga gcaggacctg gagataaaga gctgataaca ttaaaaggat acaaattatt
    21721 aagtaatgca gatgtagtga tatatgcagg ttcacttgtt aatcctgaac ttttggaata
    21781 ctgtaaagaa gattgccaaa tacataatag tgcacatatg gatttacaag agataataga
    21841 tgttatgaga gaaggtatag aaaataataa atctgttgta agactgcaaa caggtgattt
    21901 ttctatatat ggttcaatta gagaacaagt ggaagattta aataaattga atatagatta
    21961 tgactgtact cctggagtaa gttctttttt aggggcagca tcatctttag gggttgaata
    22021 tacagttcca gaaatttctc aaagtgtaat tataacaaga atggaaggca gaactccggt
    22081 tccagagaaa gaatcaatac aatcatatgc taagcatcag acatctatgg taatattttt
    22141 atctgttcaa gaaattgaga aagttgtgtc taaattactg gaaggtggat atccaaaaga
    22201 tactcctata gcagttatat acaaagcaac ttgggcagat gaaaaaatag ttaaaggaac
    22261 tttaagtgat atagctgtaa aagttaaaga aaacaacata aataagactg ctttaataat
    22321 ggttgggaga tttttaggag aagagtataa taattcaaag ttatatgata aggatttcaa
    22381 acatgaatat agagggtaat attgctataa tctgtattac agaaaatgga aaaaatttag
    22441 ctgtaaagat aaattctcta gtaaataact gtgttatata tcaagtaaaa aataaaaaaa
    22501 gtaatttagt tgttgaagaa acatgtgtac atacagttca aaagaagtta agtgattttg
    22561 taggagaaat atttagcaaa tttgattaca taatatttat aatggctaca ggtatagttg
    22621 tacgaagtat agcaccatac ataataagta agtttagtga ccctgcaatt ttggtaatgg
    22681 atgaaaaagg caaaaacgta ataagtttat tgagtggtca tattggaggg gcaaacagct
    22741 tgacacagta cataagtagt cttattgatg ctaatccagt aataacaaca gctactgata
    22801 taaatcaaaa agcttcctta gatatgatag caaaaaaact aaatgcacat attgataatt
    22861 ttagagaaag tgtaaaagaa gtaaattcta tgctagtgaa taataaatgt gttggattat
    22921 atgttgatga aggatatcaa gttgatacta ggggatttaa acttatagaa agtaaggatg
    22981 agttaagaga tagcttttca aacacatata tagaaaaatt agttgtagta agcaataaaa
    23041 taaattgtat tgaagatatt gagaatttaa acttagataa taaatttgaa cattgctata
    23101 gtactaaaaa aataataaag gtaattccaa gagatatagt gataggaatt ggctgtagaa
    23161 gaaacacaga tagtgagctg atgtataatt cacttgaaga gtttcttaat aaacaaaata
    23221 tagatattag agcaataaac acagttggaa gtatagaact gaaaaaagac gaaaaagcaa
    23281 taattgaatt atcaaaaata ctaaaagtgc catttaaaac attttcaata gatgaaatat
    23341 ctaaggttga taatcttttt gataaatctg attttgttaa aaaaaatgtg ggcgtgtatt
    23401 cagtagcaga acctatagca tatcttttga gcaaaggtaa tttgttagtt gaaaaacata
    23461 aatataaagg aataactttt tcagtgggga gaataaaaat atgatatacg ttgtaggaat
    23521 tggaccagga agtaaagata caatgacttt agaagcaata aaagcaatag aagattctga
    23581 ggctattgtt ggatataaga cttatataaa actaatagaa gaatttatac aaggtaaaga
    23641 aataatccaa aatggtatga ggcaagaagt tgatagatgt aaacaagcag tagaggaagc
    23701 gaaaaaagga aaaaaagtag cagttgtaag cagtggtgat gctggaatat atgggatggc
    23761 aggtctcatt ttagaattaa tatctaaaga agatgaagat ataaaagtaa aggtaattcc
    23821 aggtgttact gcaagtattg gtgcagcagc tatacttgga gctcctatta tgcatgattt
    23881 ctgtcatata agtttgagtg atttaatgac tccgtgggaa gtaattgaaa aaagattgag
    23941 attggctgct gaagccgatt ttgtaatttg tttgtataat ccaagaagta agggaagaag
    24001 tgaacatcta agcaaagcat ttaaaataat gggtgagttt aaaggtggag atacacctgt
    24061 tggagtggta aaagatgtag gtagagaaaa agaagaaaag tttgtatgta cttttgaaaa
    24121 tatggacttt gaaaaagtcg atatgactac aatggttata ataggaaata agtctaccta
    24181 tattaatggt gaattgatga taactccaag aggttataca gtatgatttt aattttagga
    24241 ggaacttcag atagtttatc tatatgtgat aagataaatg aactgaaaaa tcaaccatat
    24301 atcctttcag ttactacaga ttatggaagg gaacttgcac aacaacatgc agagaatgta
    24361 attttaggta agcttggaaa agaagatatg ctaaaattta taaaagataa taatatagta
    24421 aaaataatag atgccactca tccttatgca gttgaagtat ccaaaactgc tattacttgt
    24481 gcaaaattat tgaatataga ttatataagg tttgagagaa aatctttaat tgaagaaata
    24541 gattatgata ataaatttat agtagataca attgaagaag cctgtgaaat agcaaataaa
    24601 atagggacaa atatattcat aggaacagga agtaaaaatt tgaacagatt tatagaggaa
    24661 ataagtgata aaaatttagt tgttagagtt cttccaacga gtgaagtcat attaagttgt
    24721 gaaaaattag gtttaagtgc agataatata gtagctatga aaggtccatt tagccagtat
    24781 ataaatgaag aaacatataa acattacaat atagatttag tcataacaaa agaaagtgga
    24841 atagctggtg ggttcttaga gaaggtaaat gcatgtaaaa ctttcaatat accagtaata
    24901 attataaaaa gagaatcgat taactacgaa aatgtattta atgacataaa taaaattaaa
    24961 gacatacttg tgtaattttt aggaggagaa tatatgaaga aagccttatt agtagtgagt
    25021 tttggtacaa gctatcatga aacgagagaa aagacaatag atatatgtga agataaaata
    25081 aaaaattcgt taaaaagcca tgattttttt agagcttata catcaaatat gataataaat
    25141 aaaataaaaa aaagggatgg aatagaaata gataatccaa tacaggcttt agataaaata
    25201 tatgaacaag gatatgatga agtaataatc caaacacttc atattatatg tggcgaagaa
    25261 tttaacaaat taaaagaaca agtagatgga tattcttcta aattcaaaaa gattgtatta
    25321 ggaagacctt tgcttacaca tattgaagat tatcaggaag ctgtagaggc aattaagcat
    25381 caaattcctc atatggaaag taatgaagca gtagtattta tgggacatgg aacacttcat
    25441 gaatcacatt cagcgtatcc agcattggaa tacatgctta gggataatgg aataaatgct
    25501 tatgttggta cagttgaagg gtatccagaa atagaacatg taataagaag attaaaagaa
    25561 ggaaatataa aaactgtaaa tttgatgcca tttatgttag ttgcaggaga ccatgctata
    25621 aatgatatgg caggagatga agaagattct tggaaaacaa tattagaaga agatggattt
    25681 gacgtaagag ttcatttaaa aggactagga gaaaattctt atatacaaga taaatttgtt
    25741 cgtcatgctg taaaatgtgc agagaatgct aagttctatg gtataggagc agggccagga
    25801 gatggttctt tacttacgat aaaagcggtt aatactttaa agaaactgga tattctatac
    25861 actccagaat caaaaaaggg tggagatagt ctagctttat caatagttag tgaatattta
    25921 cctgaatcag tggaaataaa gtctaggcat ttcccaatga gttttgatgg gaatgaaaaa
    25981 agtttagctt gggacaatgt agcacaagaa attatatcag atgttaatga tggtaaaaat
    26041 gtaggatttg ttacattagg agaccctatg atttatagta catatgtata tataatgaaa
    26101 agaatattag gagaggtaga agtcgaaact ataccaggaa tttcatcatt ttccaatata
    26161 gcatctaatc agaattttcc attagtcatg gataaagaag ctttagtggt aattccttgt
    26221 actatggaag atgataagat agagtatgct ttgcaaaatt ataattcaat agtattgatg
    26281 aaggtatata aaaattttaa agaaataata aaaaaattag aaaagtatga tttaatagaa
    26341 catgctattt tagtaagtaa ttcttcccaa gaaagcgaaa atgtgtttac agatttaaaa
    26401 gaagcgcacc ttgaagataa gatatcttat ttttctacta tactagtaaa taaagataat
    26461 aaaataaaat aggtgaaagc aatatttaaa ggagacttaa tatgaatata gtggttggaa
    26521 ctaggggtag tactctagcg cttatacaaa cagagtgggt aataaatgaa ttaaaaaaga
    26581 agtatccaga aattagtttt gagattaaaa tcataaagac aaaaggagat ttaattcaaa
    26641 atgtttcact ggataaaata ggtgataaag gattgtttgt caaagaaata gagcaacaat
    26701 tattagatgg aaaaatagac atagcagtac atagtatgaa agatatgcct tcttatttag
    26761 cagatggact aaaatttgct catacgccta agagagaaga cccaagagat gttctaatac
    26821 ttagagaagg atataaaaat ttagatgatt tgcctcatgg agcagtaata ggaacaggaa
    26881 gtaaaagaag gaagtttcaa ttattgaaac aaagacctga tttaaatata gtacaagtta
    26941 gaggtaatgt ggaaactagg atacgaaaaa taaaagatga aaacatgcat ggtattgtgt
    27001 tggcagcatc aggaataatt agagctaatt tgcaagataa aataagttct tatttacctg
    27061 ttgatgttgt tattccagca cctgcccaag gagctttagc tatagaaatt agaagcaatg
    27121 atagtacgat agagggaata gtaaattcac tgaaggatga gaatacagaa atccaaatat
    27181 tggctgaaag aggtttttta gatggtgtaa atggaagttg tcatattcca atggcagctt
    27241 attgtgaaat aatccaagat aaaatacatc ttactggctt atatggagat tcagaaggaa
    27301 aaaaagtagt aatcaagtct atagatggag atatttcttc tcctagagag ttaggtttaa
    27361 agttagctaa acttgtattg aaggagtatg agaattatga agggtaaggt ttacttagta
    27421 ggggcaggac caggtgatta taaactcatg actttaaaag gtttagagtg tataagaaaa
    27481 tcggatgtaa tagtatatga tagattagca aatagtagtt atttaagaga agctaagcct
    27541 gattgtgaac ttatatatgt aggcaaggca tcaagtaatc atattttgcc acaagaagat
    27601 ataaatagaa ttatagcaga aaaagctaaa gaaggaaaaa ttgtaactcg tcttaaagga
    27661 ggagaccctt atgtatttgg cagaggggga gaagaagcag agactttagt agatgaagat
    27721 atagaatttg aagtagttcc aggtataact tctgctatag gaggtttatg ttatgcgggt
    27781 attcctataa cacatagaga ccatgcttcg tcatttcatg taataacagg tcatttgaag
    27841 ggtgatgata gtggagaatt aaactggaat gctcttgcaa ataacaaagg tactcttgtg
    27901 tttttgatgg gaatttcaaa cctagagaaa ataagtgaaa acttaatgaa agaaggaaaa
    27961 gataaggata cacctgttgc tcttataagt tgggcaacta gatataatca aagagttgtt
    28021 acatctacac ttgagaatgt ttatgaaact gctattaaag aagaagtaaa accgccaact
    28081 cttatagtag ttgggagtgt tgtaggactt agagaaaagt tgaacttctt tgaaagtaag
    28141 cctttatttg gaaaaagtat tgctgtaact aggtctagaa atcaaaatag tgtattggta
    28201 gaaaaaataa tggatttagg tggaaatcct atagaaatac ctactattaa ggtagaaaaa
    28261 atacaaaata atattaactt agaaaatgaa ataaaaaata taaataagta taattattta
    28321 atactaacta gtaaaaatgc cgtagaaatc ttctttgaaa agatatatga aatgaatttt
    28381 gatttaagga tattatctaa tttaaaggta tgtgctatag gttctgctac atcaaatgag
    28441 ttgaaaaaaa gaggaattat agcggatata gtaccaaaaa aatttgtagc agagtcactg
    28501 tatgaagagt tagctcctat tttaaatgat aacgataata tacttatacc tagagcagaa
    28561 aatgctagag attatctggt tgacaaactt aaggctattt gtgaagtaac agaagtacat
    28621 atatatagaa cagtaataga tgaggataaa aaaggtgaga tattagatat tttaaattct
    28681 aatgatatag attatataac atttactagt tcctcaacag taaataatct tgttgaaatt
    28741 ataggaaaag aaaatattaa aatattagaa aaaataaaaa cactatctat aggtcctata
    28801 actacagaga caataaatag tttgggaata aaattgtaca aagaatctgc tacatctact
    28861 atagattcgt tagtagattt aattataaaa aattaatttt ggaggcttga atatgttaag
    28921 aagaccaaga agactaagag caagtaaagc aatcagaaat ttggtaagag aaactaaggt
    28981 gaatgtagaa gatttaatat atccactgtt tgtagtagag ggggaaaata taaaagaaga
    29041 aatcaattct ttaccagatg tatatcattt atcaatagac atgctagaag acgaaataaa
    29101 agaaataagt gagttgggaa ttgaacatat aatgttattt ggtgttccag atgaaaaaga
    29161 tgagtgtgct tcacaagctt ttaatgataa tggaatagtt caaaaggcaa ttagaaaggt
    29221 aaaagaaata aattcagata taaatgtaat aacagatgtc tgtatgtgcc aatatacaag
    29281 tcatggacat tgtgggattt taactgatac tggttatgtt gataatgata aatcattgga
    29341 gtatttggct aaaatatctg taagccatgc taaagcagga gctgatatgg ttgctccatc
    29401 tgatatgatg gatggaagaa tacaagcaat aagagaagct ttagatgaaa atggattaga
    29461 aaatgttgga atcatggctt atagtgttaa gtatgcttca aattattatg gaccttttag
    29521 agaggcagct cattcagcac catcttttgg agatagaaaa acatatcaaa tggacccagc
    29581 aaatagcaat gaagctataa tagagactga acttgatata ttggaaggtg cagatatact
    29641 tatggttaaa cctgcattat catatttaga tattataaga agagttaaag ataattttga
    29701 cttacctcta gcagcttata atgtaagtgg agaatactct atgcttaagt ttgctgtgaa
    29761 ggaaggttta ttaaatgaag atgtaattct tgaatcaatt acatctataa aaagagcagg
    29821 agcagatata ataataactt attttgctaa agacattgct aaactgttga aaaaataaat
    29881 tctagtagta taaagaatat caaaaagtat aggaattgta attaactatt atacagtagt
    29941 tttgtttaga catttattga agaataatag aaactaattt acaatttcta tactttattt
    30001 atgtaactaa ataaaatttg gaggtagaag atgctatatc ctattaattt aaaattagat
    30061 gagttagatg tagttataat tggtggtgga gaagttgcat ataggaaatg taaaaatttt
    30121 ttagagttta acaaaaatgt tactatagtt tctaaacaaa ttttaaataa attttatgat
    30181 ttaaaaggaa atataaaaat aataaaagat gattataaag aaatatatat aaaagatgct
    30241 tctgtaataa ttgctgccac aaataataga gagttaaata tggaaatagg attatattgt
    30301 agagcaaaaa ataagctagt gaatgtagtt gataatatag aaatatcgaa ctttactgtt
    30361 ccatcatata taaaaagagg agatttactt ataagtgtgt ctactggggg caagagtcct
    30421 tcattatcat caaagataaa aaaagaaatt gaagagagat atactgaaga ttatgaggag
    30481 tatttaaatg tattaggaga tataagaaaa aaggttataa aaaaatacga agataaaagt
    30541 aaaagaaaag atgtgcttaa tatgttggta acacttgacc tagaagaact taagaagttt
    30601 gatatataaa aaataaaata aaaaatgtta aaaaggtgtt gacggattat ttttaagatg
    30661 atatagt
//
LOCUS       DAMKPG010000042        29651 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMKPG010000042 DAMKPG010000000
VERSION     DAMKPG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.045"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(148..2118)
                     /gene="ftsH"
                     /locus_tag="PC621_001987"
     CDS             complement(148..2118)
                     /gene="ftsH"
                     /locus_tag="PC621_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF4109146.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2152..3540)
                     /gene="tilS"
                     /locus_tag="PC621_001988"
     CDS             complement(2152..3540)
                     /gene="tilS"
                     /locus_tag="PC621_001988"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF4109147.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3723..4151)
                     /locus_tag="PC621_001989"
     CDS             complement(3723..4151)
                     /locus_tag="PC621_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF4109148.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4168..4974)
                     /locus_tag="PC621_001990"
     CDS             complement(4168..4974)
                     /locus_tag="PC621_001990"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF4109149.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5176..5661)
                     /locus_tag="PC621_001991"
     CDS             complement(5176..5661)
                     /locus_tag="PC621_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF4109150.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5712..6371)
                     /gene="spoIIR"
                     /locus_tag="PC621_001992"
     CDS             complement(5712..6371)
                     /gene="spoIIR"
                     /locus_tag="PC621_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF4109151.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6516..7196)
                     /locus_tag="PC621_001993"
     CDS             complement(6516..7196)
                     /locus_tag="PC621_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4109152.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7180..8070)
                     /locus_tag="PC621_001994"
     CDS             complement(7180..8070)
                     /locus_tag="PC621_001994"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF4109153.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8188..9738)
                     /locus_tag="PC621_001995"
     CDS             complement(8188..9738)
                     /locus_tag="PC621_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF4109154.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9832..10098)
                     /locus_tag="PC621_001996"
     CDS             complement(9832..10098)
                     /locus_tag="PC621_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF4109155.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10246..11106)
                     /gene="yabG"
                     /locus_tag="PC621_001997"
     CDS             complement(10246..11106)
                     /gene="yabG"
                     /locus_tag="PC621_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF4109156.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11190..12128)
                     /locus_tag="PC621_001998"
     CDS             complement(11190..12128)
                     /locus_tag="PC621_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF4109157.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12238..12399)
                     /locus_tag="PC621_001999"
     CDS             complement(12238..12399)
                     /locus_tag="PC621_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109158.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12409..12906)
                     /locus_tag="PC621_002000"
     CDS             complement(12409..12906)
                     /locus_tag="PC621_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF4109159.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13021..13731)
                     /locus_tag="PC621_002001"
     CDS             complement(13021..13731)
                     /locus_tag="PC621_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF4109160.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13907..14437)
                     /locus_tag="PC621_002002"
     CDS             complement(13907..14437)
                     /locus_tag="PC621_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109161.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14454..14684)
                     /locus_tag="PC621_002003"
     CDS             complement(14454..14684)
                     /locus_tag="PC621_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109162.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14699..16093)
                     /locus_tag="PC621_002004"
     CDS             complement(14699..16093)
                     /locus_tag="PC621_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF4109163.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16219..17472)
                     /locus_tag="PC621_002005"
     CDS             complement(16219..17472)
                     /locus_tag="PC621_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4109164.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17523..18677)
                     /locus_tag="PC621_002006"
     CDS             complement(17523..18677)
                     /locus_tag="PC621_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF4109165.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(19236..19805)
                     /locus_tag="PC621_002007"
     CDS             complement(19236..19805)
                     /locus_tag="PC621_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4109166.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20008..20235
                     /locus_tag="PC621_002008"
     CDS             20008..20235
                     /locus_tag="PC621_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF4109167.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20454..21197)
                     /locus_tag="PC621_002009"
     CDS             complement(20454..21197)
                     /locus_tag="PC621_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF4109168.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21365..22237
                     /locus_tag="PC621_002010"
     CDS             21365..22237
                     /locus_tag="PC621_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4109169.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22237..22902
                     /locus_tag="PC621_002011"
     CDS             22237..22902
                     /locus_tag="PC621_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF4109170.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22923..23780
                     /locus_tag="PC621_002012"
     CDS             22923..23780
                     /locus_tag="PC621_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4109171.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23936..26482)
                     /locus_tag="PC621_002013"
     CDS             complement(23936..26482)
                     /locus_tag="PC621_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4109172.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26482..27168)
                     /locus_tag="PC621_002014"
     CDS             complement(26482..27168)
                     /locus_tag="PC621_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109173.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27251..28240)
                     /locus_tag="PC621_002015"
     CDS             complement(27251..28240)
                     /locus_tag="PC621_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109174.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28227..28913)
                     /locus_tag="PC621_002016"
     CDS             complement(28227..28913)
                     /locus_tag="PC621_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109175.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29413..>29651)
                     /locus_tag="PC621_002017"
     CDS             complement(29413..>29651)
                     /locus_tag="PC621_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109176.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         9655 a         5370 c         3411 g        11215 t
ORIGIN      
        1 aaataatttt aaaataataa aaaactgaaa gtatcaatta aattaataca tgcaaatata
       61 atttgcttat taatttaata ttatactttc agttttagta tttaatccac atcagataat
      121 aatcttaaaa tgaccaaggt cttaaaacta tttatcttta tcagatttat cttctaaatt
      181 tttatttata tcaataacat tgtttctttc ttcttcatct ttttctaatt ttatagttaa
      241 ttgttcttca acttctttag aagaattttc ctctgtaact gcattttcaa tttcattcag
      301 aggtaactct ttattaaatg cttttacaaa ttgttctgca tctaaagttt catatataag
      361 taaagcttgt gctacatact ctaatctatc catattttct tgtaatatac ttcttgtctt
      421 atcatatgca ccatcaacta tacgctttgt ttcttggtct atctcaaaag caacttcttc
      481 agagtaattt ctcttacttg aaaaactatt acctaggaat acttcatcat cactgtcaaa
      541 tgacattgga ccaagtttag aactcattcc atactttgtt accatacttc ttgcagtagc
      601 tgtaactctt tctaagtcat ttgaagcacc tgtagataca tccttaagta ctagctcttc
      661 tgctactcta ccaccaagta aaactacaat attttctttc atttcatttt ttgtagcata
      721 gaacttatct tcaacaggaa gttgcattgt aaaccctcca gctcttcctc ttggaactat
      781 tgtaacttga tgaacaggac ttacttcttc aagtacatgg gcacaaactg catgccctcc
      841 ttcatggtat gctgtaagtc ttctttcttt ctcacttatt actttcgatt ttttagccac
      901 acctgctata acctttgtta tagcttcttc tatagtttcc atcttaattt tcttttctct
      961 ttttctagct gttaatattg cagcctcatt cattaagttc tctatatcag caggagtaaa
     1021 tcctggtgtt cttctagcca agacatccat ttttacatca tcacttaatg gtttatttct
     1081 tgagtgaact ttaaatatag cctctctacc ttttacatct ggtgtaccaa caacaacttg
     1141 tctgtcaaat cttcctggtc taagtaaagc tgggtcaagt atatctggtc tatttgtagc
     1201 tgccatgatt attatacctt gatttacccc aaatccatcc atttcaacta gaagttgatt
     1261 aagagtttgc tctctttcat catgacctcc accaagacct gcacctcttt ttctaccaac
     1321 tgcatcaatt tcatctatga atatgatagc tggagcactt ttcttagcct gttcaaataa
     1381 atctcttact cttgaagcac caaccccaac aaacatctca acgaaatcag aaccacttat
     1441 actgaaaaag ggaactcctg cttctcctgc aactgctctt gatagatatg ttttaccagt
     1501 tccaggaggt cctaccataa gcattccttt tggtattctt gcacccaatt caatatattt
     1561 cttaggattc tttaagaaat ctactacctc ctgcaaatct tctttttcct catctagtcc
     1621 agctacatct ttaaatgtaa ctctagtttt ttcatcatct ttatgaacct tagcttttga
     1681 tttgccaaaa ctcataacct ttccgcctcc accttgagac tgattcataa acacgaacca
     1741 aataatcacc atgaagaata ttaacagtaa tgtaggcaac atttcgacaa accaaggtgt
     1801 agatggctta gcttcaccac caaaagttag tttcccagcc ttagcttggt ctaacacctc
     1861 atctgcaagt ttatttccca tcacttctgt tggaacatat gacttaaact ttgtatttgt
     1921 atcttttata gtaccttcta cagaagtttg atttacaaag tataatcttg atatattctc
     1981 atctgttaat tctctatata cttcagagaa ttttaaatct tttatttttt ctgtaggctt
     2041 acctgagaat tgaactatac ctactataat tataaaaaca agtagataaa aacctgcccc
     2101 ttttagcaac ttattcaata tagaagccct cctttcatac attgctttgc cctataattt
     2161 gttaaaatta atttttaata cttcttttgt gttttcatca attttataat tttcactgat
     2221 tttataatct ccaacacata ttattccttc actatctgtg attataggaa ttttacatct
     2281 ttcttctctt gggattttta aatctatgaa taaatctttc acttttttac ttcccatagc
     2341 aagctttatc ttatcacctt gccttctgct tcttattact ataccccctt ttaccttatt
     2401 aaaatcaaac cctttagatg aattgtctaa tttcatactc ttatacctat ctatgctcat
     2461 tacttgggtc tcaataattt gttttaattc ttttatttta ataaacccat tactaggtac
     2521 attgtaataa aattcaattt cctcattaac aatttcctca ttcgttaata ttatactgtc
     2581 tttttttcta taaacaaata tatttcttgg aagagttaac attttattta cttttgaatt
     2641 atcttctaaa gacattatat cttctatatg tctttggtca acaaaattag tatctcctag
     2701 tatatgtttt atggaatttc taagaactct aactttgata gcatcatgta agcaaacaaa
     2761 atcgtctaaa tttatctcta caaagccttt ttcttttata tttgaaactt ctctaaattt
     2821 agcttctgcc tctttctcaa tataatcgtt atcacttttt aaactattgc tcatcctcac
     2881 tatagattct attacatttg aattaaaatt atccttcata tatggtaaaa ggtctaacct
     2941 tatcttattt ctagtgtata tattttctaa atttgtttta tctatcctcg gatttaaatt
     3001 ataagcttca caatactcct ctatctcatt tctttccacg tctaatatgg gtctaattat
     3061 gcaattatct ctaatataat caattccctt taagcctttt aagcctgttc ctctcatgat
     3121 acgcatcata acagtttcgg cttggtcatt gagattatga cctattgcta ttttattggc
     3181 tttaatctta tccttaattt cataaaacat ttcatatctt agcttccttg ctccttcttc
     3241 taatgataat ccctcatttt cacaatattt tggtacatta atagacttta caaagaatat
     3301 aattcccata tcctcacaaa gtttagatac atataatgca tctttttgag cttctatccc
     3361 tcttatttga tggtttaaat gtgctgcata tatttctata ttaaaatctt tttttaatct
     3421 atttagtaca tgcaataaac aaactgaatc aggtcctcca gaaagaccta aaactatttt
     3481 atcgcctttt tgtattaaat tatgtttatt tatagtactt agtaccttat caaatatcat
     3541 atttatccct cttacactta gttttattca cttttaagta tactttctat aaaaaaacgc
     3601 taaacccttg tatcaatttt aagaaattaa agtttaatat aaatataata ataccaaata
     3661 aattaaataa aaggtagttt tttcactacc tttataaaat ttctggaaaa tagccatagt
     3721 tattatttag tacctatgta tatatctatt ttattcctac ctaaaaataa atcttgtacc
     3781 tttatattat agtctatttt caattttatg tgttcccctt cttgtatatt tatatctaat
     3841 tctcttgtat caatatcaat taataaagta ccttgtgggg tcacatactt tgtagtacta
     3901 ctttctccac atttaaacag cattgttgag ttaatagcac caaacttttt gatactaact
     3961 tgatttttaa gtatctttat agtattagtt atttctatat tttcttcttt ttccttgtaa
     4021 ataacatata ttccatcatt tttataaaaa attttaccaa aggcagtcat ctctatggtg
     4081 tccatatttc ctttttcatc atattgtcta gtatttatac ttatttttac actcaacgat
     4141 ttttcctcca tagattcttc atataaacta gtatttttgt atttctactt tttgaatctt
     4201 atcaattttt ttctttaaga actctcttgc tatatctgaa aatttttcag gtatatctga
     4261 tacataatat tgataagtcc catgaatttc tgtattgttt ataatatttt gcacctctaa
     4321 aatctttttt aaatcctttg cagtctcttt agctggatta acaagtttta tatgctctcc
     4381 aacttcttca cctattgttc tctttaaaat aggataatga gtacatccta acactaaaga
     4441 atctatattt ttttctttca attcttgtaa atatatcttt gctgtcaatt tagccacttc
     4501 tgtatttgcc catccttctt caacaatagg tacaaataaa ggacatgctt tattaacaat
     4561 ttctatactc tcatctattt tagatatttc caagttgtat gcttttgaac ttatagttcc
     4621 ttcagttcct attattccta caattttgtt ttttgtagaa cttacagctg ttctagctcc
     4681 tgcttctata actccaatta tcggtatatc atatttttct tgagcttctt ttaagcttct
     4741 tgcagtagca gtgttacatg ctataactat agcttttaca ccttttgttt ttaaaaagtt
     4801 tatagcttga aaagtatatt taattatagt ttctttggat cttgaaccat atggtattct
     4861 ggcagtatct ccaaagtata ttatatcctc atttggtaat atcttcatta tctcttttag
     4921 aactgttaat ccacctaatc ccgaatcaaa aactcctatt ggtttattgt tcatctttat
     4981 acaacaccta ctttacattt ttgtgtttaa caacaactta tttatcgcta aaatttatat
     5041 ttatattcat aattaatata ttattaccta aatgattatt atagtttatt ttacacaaaa
     5101 aataaaggag ctatcttaaa ttagaaaata atttctacaa aaagacaact cctttcacta
     5161 cttaaaaatt taaatttatt ttgcaaacac atgatttcct attctcatat aaggattcaa
     5221 tctatttatc catttgcaag tggctatttc tggattccaa aaatatactg ctttattagt
     5281 tgggtcagtt ccataaagag cttcctgagc tgctttacgt gctgagtctg ttggtgccat
     5341 ctttattgaa ccatcaagca caactgagaa agcatttttt tgatatatga cttcttctat
     5401 tgtgtctgga aatctagggt ccaatactct atttattact acagctgcta cagcaacctg
     5461 tccctcatag ctttctcctc tagcttctcc tgcaaccaac ttagataata acatcaagtc
     5521 attatcagat aaatttatta cttctttctt tgctatattt ttttcttgtt tgtttacttt
     5581 cttaatattt tctgttggtt catatgcaaa tacctcttta ataggcataa atataaaagt
     5641 caaagctaat agtgtagcca tcagtttact ccacctgtcc atgattaaaa ccactcctta
     5701 cattcttatt tttaaaattt tttctctaaa tccttgacta cttcaagtac tttaaactta
     5761 atttttactc tacttgtttt ttttggtgta tcttgtttta ttaacttata ttctttatca
     5821 gttaaaactt ccttcaactt atcatctgta gatttatcta tcactccatt agattcatca
     5881 acaaaacata gtggagggaa cataacacac caccagtttt ttccttcacc tttcccaatg
     5941 ataatcttta atgctttata ttcaccagct ggaagaacta tattagaata ttgtttagta
     6001 gggaaattgc tatattgtat acctaccttt acactgtaat tatatccatt tttaagaata
     6061 atatttttac ttatttcttc cagactacta tattcctttt ttataatagc ttcactttct
     6121 tttatacttt ttgaattttg aagctttggc tgtaaatatt ttattacttc atctctaact
     6181 tttagtttaa gttcttgatc ttcatctgta ttgctgtttg ctataacatg aaatcttatt
     6241 aatttatctt tataatcttt tgatatattg tctacttttt ttacctcacc atttattact
     6301 attgtcatta ttgatatgac tgatataaga cttaatatta atagtcccaa tctaatctta
     6361 atttttctca ttgaaaattc cccctcaaga taaatttata tcatcaatta tgtccaatta
     6421 aatttaattt attcataata ttttttaata ctaataaaaa aagctatctt aaaatgatta
     6481 caatcatttt aaaatagctt tttaaacctt atattttata agtctttact aatcatagcc
     6541 ttatctatca taaactgctc tgctttttct atatgctctg tagcatactt cttggatagc
     6601 tcactatcac ctttaattat agcatcaagt aagtatttat gctcttcaac aatattatgt
     6661 gatgtatcat aagcagacat ataggtaact ctaaatctat atacttgttc ccataatgag
     6721 tttattagtt ggattaattt tttattatta gttgctttaa atatacattc atgaaattct
     6781 acatcaagtt ttaataacgc atctacatcg gattccaaag tgcttttttt aaattcttct
     6841 gatatatctt taagcttttt aatatcttca tcactaattc tttcagcggc aagatatgct
     6901 gctaagcctt ctaggcttgc tcttacttct aatacatcca taatatcctt taatgacata
     6961 tttgccacat acgcaccttt tctaggcaac ataacgacta aaccttctaa ttcaagcttt
     7021 cttattgcct ctctcactgg agtcctactt actcctaatt gttctgctag ttgcacttcc
     7081 attagccttt gaccaggctt taattttcct tctaagattg cttctcttaa attctcaaag
     7141 actacatctc ttaatggctt gtaattatcc aagttcagtt tagttaaatt ctccacaaat
     7201 ttcaacccct ttttgactgc tattaacaac atacacctgt ttatattttt ttaataattc
     7261 tttttttcca attagagcat cttctttatt tttaaatagt ccaaaaactg taggaccact
     7321 tccactcatc atagaaccta aagcattatt tttcatcatt acttgtttta tatcacttat
     7381 ttctttgtgt ttacctatag taacattttc aagaatattt accatgcttt ctgatacagc
     7441 ttttatatct tcagatttta aacactctat caaatattta ttatttggtc tttttttaat
     7501 attattcaaa tctaatcctt gataaacttc tttagtagaa acgaataagt ctggtttgca
     7561 tatcaatata tttgtatcac atggtaaacc tttgatgttt gttagctttt ctccaatacc
     7621 ttgagctaag gcaggtcttc ctgatataca aaaaggaaca tctgcgccca aattaagtcc
     7681 tatttctttt aattcatctt cagataatct aagttcccat aggtgattta aacctactaa
     7741 tactgcggct gcattagaac ttcctccagc catccctgct gctacaggaa tatttttttc
     7801 tatgaatatt tctacacctt tttttatata aaatttattt ttaagtattt tggcagcttt
     7861 ataaacaata ttatcttcat ctaaaggaat atctaaacta gtgcttttta cctttatttc
     7921 atcttcgtct aattctttta tctttactat atcgtataaa tctatcgttt gcattatcat
     7981 ttcaacaaaa tgataaccgt cttgcctttt tcccaagaca tcaatggata aatttatttt
     8041 tgctctactc tttagtctta taaaatccat tctatcctcc ttgtttaaag aataatgcat
     8101 cactaacaat aatattatac tacaaaaaat aaaataatag ggtattataa taccctatta
     8161 tacttaatta ctttaaattt tttaatatta atctactaat acgacttttt tttctaaaat
     8221 aagacactta ccaggcttaa ttggttcatc aagttttata tcattaagtt ctgcaatttc
     8281 attttctgtt gtattgtatt tttttgctat attccagaaa gtatctcctt ctttgcatat
     8341 atatacgatt atgctaggag ctttagataa atcatataca ccttggtctt ctcctttgat
     8401 aataaaattt tctgctttct tatctaatgc ttcagtaaat ctcttaattt tgataatcaa
     8461 gtctatctgg tccctattca aatcaacttc gactttatca atacatgctg tattaaacac
     8521 agatgctgta tcagttagat tatccatttc tatatcatgt tcaaatggaa tttcctcact
     8581 aattttataa accaatttta aaccttcaac tgggacaaat aaaatttcaa cttttatgat
     8641 tccttgtata atgcttttat ttccttcaat ataactattt tctatagaca tggttggaca
     8701 aacgcttaca atatctttta tttgtatatc atcattatca tttcttatgc cttctctaac
     8761 cataaatgtt tcttcactac gtcttaaagt cttatttaat tgaataggtt tgtggtcaaa
     8821 ttttattatc ttttgaggcg aatatgcatc ttgtaatact tctcttgtaa cttcatctgt
     8881 aacttttacc ttacaacaag ccatacaatc tatttctaaa agacctgtgt tgctttcact
     8941 attttgcttg aaaatatgat taaaatctga catagacaac aatacttctt cagtcatacc
     9001 atcactaact ccagggactt ctacaaattg agtaaactca atacctactc tatccaactc
     9061 tacaagttca ccctcatatg tacaagctaa cggatttatt tctaatactc caccaataat
     9121 aactttattg tcagtcactc tactttcttt tactttaaca catggattta aacttattat
     9181 agattgaatt tcctctgtgt ttattgttat agtatctctt atagaacttt ctgctttttc
     9241 tattccaact atatcttgaa aacaaatttc ctttctgtgc ttttgtatgc cttccacttg
     9301 cgccacatct tttacaatgt ctaacctttg cttttcaaac aaacttcccc ttatattcat
     9361 aagagctcct actttaatct ttctctcatt cattatagta caatccatat gctctacttc
     9421 tgaaaataac atgtattcca tatcttgaac aacattgtct ttttctatta cttcgtttat
     9481 atctactttt ccgtctacat tagaaatagt atttttatca tctgcaatat agattacatt
     9541 ataattaaaa ctacctctgc aaagtatctt cccatctgcc atttcaattt ttttaagtga
     9601 tatgtatcct tctgttttta caatttcata tacatctaat tttttgtctg gtacaactgc
     9661 ctccgcttca atgaatgttt gaaattttcc aaagtctatt ctattgtcaa ctttaattac
     9721 atctttaatt aattccatag tctacctcct taagtattat aaaaatatca cactatattt
     9781 atatttaagg agcaaaaaaa aaattacacc tatttttaaa taggtataat tttaacttat
     9841 ttgtaattta tcttgatctc taaaaacttg caactttaca ttagatgtta ataaatctga
     9901 gtaactataa gatactctag gatatccatt actttcatca tctaacttta taacaaacac
     9961 gcttggataa actttttcta gtataccctc ttttgtaata atctgctttc ttcctttatt
    10021 agctttcaac aatattttct ttcctatatg tctctctaag cttactctta tcttatctag
    10081 agtttgaaca gtagccacaa tatcaccttc tttaacgttt atatgtttac ataatatcac
    10141 atataaacat ttttgtcaaa tgtttaaact aattatttta tatatttttt gcaaaaatgt
    10201 caatactatt tttgaagata tacagtataa ttatgttaat ttttgctaat gtaatattgt
    10261 ttttggcatt gcccatctaa attttcctct tgtttcagaa ccaccaattc ccttaacacc
    10321 tgtaatagtc tgctctataa cctcatcaat aggaactact acatcaattc ttgaacaagc
    10381 aattttctcc actataaaaa cagggtctaa tgcatgtatc attattcttc caggcgaact
    10441 tgcataattt gctcctgcac ttattatttt ttcataattt gattggcatg ctcctgcgaa
    10501 tatgacaaga ttatccaaat ttggttgcca tttacgtgct tcttttactg ttttaacaaa
    10561 atttgaagag tttctatagt tactcatatc ttctatatct cctcttttta ttgtcattgc
    10621 atcatgacct gttattacta gtatatccgg attatatttt tctagtagtg ctcttacttc
    10681 tttatactga ttttgctctg aaacagctac acctactgca ggtattccaa gttgagcata
    10741 tacatccaag caaattttta aatattcttt atctccatct atctgtagta cttttccagg
    10801 cattccatat gtgttaggat ttaattgaag ttttggaact gctctagtca ttcttttttg
    10861 tctttcttta gcttttctta ctgacttata taataaattt tctacatttt tatctattaa
    10921 tatatctttt atatcaggtg cttttactaa ttctaggtca tcaatataag catctgctat
    10981 tactctaaat gcaattccct ttagtatagc tattttctca ttattttcat ctacaccaaa
    11041 agagactatt ttaaatacta tatctttgtt atgtgacttt ctagctacaa tgtctcctac
    11101 cttcatagtt aaacctccaa taaattttta tcttagttta tactatgttc gatataaaac
    11161 tttgtttcct aatgtcaaaa aaacatttct tataatactt cttttatcac tcttaaagca
    11221 tttttatagt atatcttttc tatatcatct tcactaaacc cttctttttt tagaggttca
    11281 tacagtttac ccatctgaga tatatcttca atctcaactt ttgaatctat tccatcaaaa
    11341 tctgacccta gggatacaac atctattcct ccaacattta caatatgttt tatgtgtcta
    11401 accatatctt caagtttact ttcactttta tcacttaaaa ataagtggaa gaagtttata
    11461 cctgtaactc cacctttatt tgctaatact ttaatcatat catcagttaa atttctagaa
    11521 tgattcatca ttgctcttga attagaatgt gtagcaatta ttggtttaga agaaatcttt
    11581 gcaatttcat aaaatccacc atctgaaata tgagaaacat caaccaacat tcccaattca
    11641 ttcatcttat gtaccacttc tcttccaaaa tcagtaagcc ctttttctct atattctact
    11701 ttattatgag gaaagctcaa ttcattcaca tgattccaac ttattgtcat cattctaacc
    11761 cctaagtcgt aaaatttctt taaattttct aatttacctt caagtactgc accttcttca
    11821 atagataaaa gagctgtaag tttaccttct gattggtttt tcataatttc ttcatagtta
    11881 gtagctaaag ctatttcatc actatttttt ttcatttcct catgaaattt atttgccata
    11941 ctcatgcaat aatcaaaagg atgttttact tcttcagtat caacaaaaag agcaaatgtt
    12001 tgagctaatg aatccccctt tttcaatctg tctatatcaa cagaatattt attagactta
    12061 agttcacttg tttctacatt ttccattaat tttgctatag tatcacaatg taaatcaata
    12121 aatttcatat ttataccccc tgtatatttt atatttatat ctaaaaatag aaatagttgt
    12181 ccttatagac aactattgac aactattttt taatattctt tttaatatta cttaatctta
    12241 ttcttctttt aagttaggta ttactttttt aacttcttca atttgatgcc atgttttatc
    12301 tatattattt tcactaatat atttttttaa atcagttaaa gatgtaaaat ctttaactga
    12361 taaattgtca tcatcatctg caaatcttaa aataaacatg agactccctt actttccaca
    12421 acatttctta tatttttttc cgcttccaca tgggcatgaa tcatttcttc ctatttttgc
    12481 ttctttaact attgttttag acttcttata agcctttatt atttcttttc tttggtctgc
    12541 tggtagtata tcttcccaac cttgtaaatt atatagccaa tgagcctcaa cagttaccat
    12601 attgttatat aatttttccc aatctatttt tatagaaact tctttatcag cttctacttc
    12661 ttctaaaact atatcttctt ttaagctttc acttattcca tctatgaatc ccatgaaaaa
    12721 ttcattagta gttccaaatt tttcagccaa ttcacttact ttaccttcaa caacatccat
    12781 tttattattt aatatttcat tgtatattcc agcttcaact ttcaaatatt cttcccagaa
    12841 ttttacttct gcttcttgac tttcatgatt atcacttaaa tttctccatt cagtatataa
    12901 actcatactt tttcctccta agtataccta tatttttata gtttttatag tataaaatct
    12961 tttagttaga ttggaagttt cttctttttg attataccat attctattta aagttaactc
    13021 ttatattata tcataattaa acaattcttt taatactttt atagcatact cttggtttat
    13081 aaaataaggg cttgtcgcaa ttgttattac tttggctttt ttaattaagt tttttatctt
    13141 gtaaaatttg agttcaaatg gtatatagtc catatcactt gaaaatatat ccaaatctat
    13201 atcaagaaca aactctgatt ctacttctaa atcaaatcca tacagactat ctattattat
    13261 taattctgaa aatatgttat attttaaagc aggttttata aaacttccca catttaaaac
    13321 ttcatttgta tacctaaata catcttttag attatttaca tctacatcat agttctcagg
    13381 ctctctagta tccttatgtt ggtctacatg cactaacttg catccttttg taaattcacc
    13441 ctttaacaaa gattttgccc aaaaataaaa tgcatggtta tgattgtcaa acacatatat
    13501 ctttttattt cccaggacat attcgaccat attctccaat ccttttgctt ttatttcctt
    13561 attttcatct acttcattaa ataccacctc attgcctaac cttacatcat ttaatgtacc
    13621 atttataagt tttggaacgt atatgctctt attttctctt ttatcatatg aaaatacatt
    13681 atttcctact ggtttttcta tataaaatcc actatattca taattttcca taaaacttta
    13741 tttcctccat tgttatataa atatataagt tataatttta aaaattaaaa tgcacttatt
    13801 gtaaatcata ttatctatta tagaaaacat cttatataac taaatataat atcttctctc
    13861 aatatgtatt atacaataag tgcaaaatat atttatataa aatatactac attcctataa
    13921 caaaaccatc agcttcaatt ggaaggtaac taactcttct attcaactcc acctttccaa
    13981 aaaatactac ccaatatggt ctaaaaatac tttcaatttt aactatttca atctctggga
    14041 ttcctcccat aaacctatga attaatctca tagccatttt atttgccttt gcttttactc
    14101 ttcgttcgtc cttattacta tactgcacaa tattacaatc cacatttttt acaatttcat
    14161 atgtaggaaa atcttgtact atactaccag aacctgtaga accattcact aaaattttta
    14221 attcatttat atcattagaa tatctcctat tcttgaaaat ggatttcgtt ttgtgaattt
    14281 taagtgtgat aattttaaac tctatataat gaagtctaac ttcatgagca tatttgttct
    14341 ttaatattag cttactaaat aaagaatttt tcccactttt agctatctct atagcttctt
    14401 cttttgtaat tcttataggt aacgctctta tatccataaa atcattctct ttcttatttt
    14461 tttcttgtca tttttaaaac tctatatata taatcaaaaa atattacaat tacaactgcc
    14521 ataactccat atggaattat ttttccaact ataatatcat ttctagtcaa tataccataa
    14581 aatataaatc ctgttattaa aaagtacaat aatactagaa aaccgtctac tccaagccat
    14641 tttatttcta aatctttttt taccttagac actaatttag acatagcagt ttctcctttt
    14701 atagattatt ttgtgtattt aaattttcac ctattctatt accaatcatc catgcaataa
    14761 atgaaaaagg tatagctatc acaagaggct ctaactcaaa gctctgaact ggaaatattt
    14821 taaaataacc aaaagtcact ccacctacaa tcattcctaa aataccaccc atagatgttc
    14881 ttcttccatt tccaagaagc ccacctacta taggtacaaa tgccgaaccc atccaaatac
    14941 ttcctacaaa tatacatgca tcatatacat tttcaaatct aaatgctacg ctaagcccca
    15001 taactgctgc taaacataca cctattctag taaactttac taattgtttc tgagataact
    15061 ctttttttgt aattcttcca tatatatcat ttgctaatgt agctccagct gctagataat
    15121 agctatccaa tgttgatatt atagaaccta aaattcctat tataaatatt actcttattc
    15181 cttgtggtaa tgtatctaaa actaaagtta tatatccagc ggctggagcc atctctggat
    15241 aagtagctct tactataagt cctgttatag ttaacaccat gtcaaaacat atccatagtg
    15301 aaaaacaagt taataaggct cttcttccaa ccttaggaga atctgctgct gaaaatcttt
    15361 gataaaaagt tgggtctgaa taagctccaa aagaatatac aattaacatc aatgctttca
    15421 tagctgacat accttttgta gggtgcataa gctgtggtgc atctttgagt gcttctgtga
    15481 gaccttgaaa tcctcctatt tgttcaaatt gacctggcat aaccatagtt aaaccaaata
    15541 ccatacaaaa aaacaatacc atatctgtaa tggcaactcc cataagccca ccaagtactg
    15601 taagtacaac gactactcct accattatta atcctattaa ttctttatct attttccatg
    15661 ctacgttacc tatagttccc atagcagtta tttctccaag ttgtgcacaa tatataaaca
    15721 tcaaaattgc cccaattata gcaacatgct taccataaaa actttccagt aaatcaggaa
    15781 ctgttatatt ggttcttata tgaatccttg gaccaaccca caatgctaaa ggcattctcc
    15841 caatatgtgc agctattgac caaactgccc atgcagatat tccatatata gccgcatact
    15901 ctacactacc tactgtacca actcctccat accatgatgc aaccaaagtt cctactatta
    15961 aggaccatgg taaacttcga tttcctaggt aataactttc catatctttt acatttctag
    16021 aaaaataata acctattcct aataatataa atgcaaatat aactataatc ccattttccc
    16081 aaaatgttaa catatctaat ctccacattc ttatatttta atttttaact ttaatatcta
    16141 aatatgaaca actctaccca tttccataaa aataggtaga gttatataat aattataagt
    16201 atttaagtaa tcttgttatt aagcaatttt attgtttaaa cctttatttt ctcttttatt
    16261 tattttattt aaagcataag tagcaaatat ccctataact gcacatacta cagataaaat
    16321 aaatatatat gtatatcctg tttgtccata tttatctaac caattacctg caatagtgta
    16381 ataaaacgca tctggagaaa atcctataaa tgaagcaaat ccaactactg ctcctgtagt
    16441 ttctaaagat atattagact ctgaaacaga tgcaaagtat atacctctaa atccaaataa
    16501 aattacactt agtattatca tgtttattac agctatccat ataaatcctg tactcttagg
    16561 aataaccaag taagcagctg tacttactgt cattaatata aatcctacaa acattactct
    16621 tatagatgaa ccaactttat ctgttataac accagcaact ggagatgctc ccatttttat
    16681 aacataagtt cttattattg ataaagcaga aactagagtc attggcatta cataaacttc
    16741 tgataagtat ggacttaaat aagttaaact tgagaatact aaataagttg aaaatattat
    16801 aagaccaatt aaccaagcct ttggcattgt aactactttt aacaatgact taatacttaa
    16861 tccttcatct gatgctccac tatctttttt ctcttccact aagaaaaata gaagtatacc
    16921 acatattata gatgctgcag aatataacca tactacatat ttgaatccta ctgttatttc
    16981 agcaaattta ccaaagaagt aaagacctat aaaagaaatt aacgtacctg atacacctga
    17041 taacccttca taaaaaccaa atattcttcc ttgttctgac tcacttcctt gcattctaac
    17101 aaccttaaca gatgctgaga agaaagttaa tattgttgta aatccccata atacaaatat
    17161 taagagtatc atactatatg atgggaatgt agagaaataa aaaccaagta ctcctgaagc
    17221 aattaaagaa aaagttatca gctttctaac cgagaatcta tctgcaaacc atcctcctat
    17281 aaaataagat accattccta ctaatccata taaactcaat aagttcccta tttgtgtatg
    17341 gctcaatgat aaagcttgtt gcattgggtc ataaaatgat gatttcatat atggtaaact
    17401 atacatagcc gttgttccaa atgccaatat gaatattatc aagaatcttt taaaattact
    17461 tttgttctcc attaattttc tctccttaaa attttattgt ttataatctc attcatgtaa
    17521 ttttatttta atttttttat tgcttgttta aagtcattta ttatatcatt tgaagattct
    17581 aatcctacag ctattcttat tagattttct ccattcattt gaactaatgt atgctcttcc
    17641 cctaagcttg ttgctattgt tgcaagctct aaagagttca ttaattttct actagcttgg
    17701 tctcttgtca atccattgat accatcctta agtttaaatg aaactacacc tccacctaaa
    17761 ccattcattt gagttttaca aagctcatat tgaggatgag attttaatgc tggatacttt
    17821 accaattcca cattctcttc ttcctctaaa aactctgcta ctgctaatcc attttcacaa
    17881 tgctttttca tcctcatatc taacgttttc atacctctta aaatcatcca tgcactcatt
    17941 ggtggtaaag atgctcctgt aaaacaacat aaacttggcc atctaactaa atctattaat
    18001 tcttttgaac ctgctactgc tccccctaaa gtatccccat gtccatttat aaacttagta
    18061 agactatgta ttactaaatc tgcacctaaa tctattggtc tttgtacaat tggtgtccca
    18121 aaagtactat caactatagc taagctttta ttttcatgag caatttctgc tattgttttt
    18181 atatctacta agtctaatga tggatttaaa ggactttcta tataaactat tttagtattt
    18241 ggtctcattg cttcttttac attttgtgga tttactgcat ctacaaatgt aacttcaact
    18301 ccaaatttag ataacaactc agacatgaat aatttagtgt gtgaaaatat tccatctgaa
    18361 ctaactatat ggtcaccact ttttaccaat gctaatatag tagatgttat tgcccccatc
    18421 cctgatgtac tagatatggc actttctgca ttttcaagca tagccaactt agcttctaac
    18481 tctgtaagag taggattaca ctctcttgta tacacatatc cccatgtcat acacaactct
    18541 tcaaaatgct ctactgtctt tgcagcaaat gttgctgttt gacatattgg tgtagccaat
    18601 gcacctgttg ttgggtctgc actatgtccc caatgtatta tttttgtctc taaatcttga
    18661 ttaatattgt tactcatttt gatttcctcc cattttgact tatttataca acgttttccc
    18721 tgccaaattt aatcaattta gcttagtctt aatacatttt ttcaagtata cttttttatt
    18781 tgttataatt tctaaaaata tgacacttat tttgtttttt tacagttcta attataaact
    18841 ctatagtaac tatagagtca atatgttttt gatatttttt ttacaatgtt taaaatagtc
    18901 agtacaaaaa taggtatatc ttagaataac taaagtcatc ctaagatata cctattttta
    18961 tatcacaata taaataatta catataagca ttagtttttt tatcataaat agatttttca
    19021 agatttatca tcttttcata aatatattat agttaaatat tttaatatta cctaacatct
    19081 acaataaaat atactcttaa aataaaatta tataattaaa aatactctat aaaagctaaa
    19141 aagttttcta ttcgctacat ctaaaaataa ttagatagag gtataaatgt atttagttta
    19201 tttatacctc tagtctttta taaattatat ctctactaaa ttgatttttt agcacgaata
    19261 agtgtgctct tggagatacc agtcatcttc acaacctctc tatatggctt agaatttaat
    19321 aaccccaatg catattgtat ctgttgattg ctgtatttct ttggcctacc atctttaaag
    19381 tcctttttct ttttagcaaa ttcttttcct aattgagtac gttctattat catatttctc
    19441 tcaagctctg caactgcaag taatgtagtc aaaaagaatt tacccattga agtattttct
    19501 aaaagaccta tatttaaaat atgaacactt acttctttag caaataaacc ttctattagt
    19561 tgtatccctt ctgatgtact tctagctagt ctatccagct tagttgcaac aagtaagtct
    19621 ccctttttta gagatgatat aattttttca aatttaggcc tgtttgtagt agtgcctgta
    19681 taagtttcct ttattatttt agcatcatta tatttattca aaatttcttg ctgttgctga
    19741 tacaaactgt tgttgtctgt ttgatttttt gtacttacac ggcaatatcc atatataata
    19801 atcatcactt cctaacataa agattttgta cagaagttac ccagaagctt gtttttactt
    19861 atctttatcc attttactaa aacgattttt gggtgtcaat actgttaact tttgacacca
    19921 taataattcc tttataattt gtttgactat gtcaaaaatt accaatataa tgaaattaat
    19981 gagtttatac aaattggagg aaataatatg aatagtctgt taaaagaagc aatagatgaa
    20041 ttaaaaaaca tccattgcgg ggaagttttt ttattaaaag aattgttcaa aggctatgaa
    20101 tggaacaggt tggagatgaa tattagattg aaattaggaa aacttttttt gtatgaagtg
    20161 gtaactaatc ccaatttaaa tgtagaggtc atcaataaaa attctgctta tcagcaaatg
    20221 tataaaaaaa aatagtgact taaatgtata taatagtata atcttcttat tgattcaaaa
    20281 gttaaaactt ttaatacaaa acaatcattc tattttaaca tttatcaata tgtctaaatt
    20341 tatataatta acacatacga atagtattgt ttaaatcaca ttatttaaga tagataaatc
    20401 ttaaccctac tccagcataa atactaaaat agggcttaga attatctata tttttatccc
    20461 ataaaatgct tagctcttcc tcttgattga acatatatat ctggggaaat acaatagaag
    20521 cgtaaattta taaaatttcc aaagtcagaa aacttcagtt caaacttagg tataccaaaa
    20581 ctttcaggtg ctatttcatc aacgccaaaa tagatgataa ttccattatc tgtaatataa
    20641 aatgcttggt cttcaggtat aactaactca acattagaat aatacatttc tctattttca
    20701 tttatttgag atttaatgta atcagtaatt atattaatat aatcaactcc atctctaaaa
    20761 atgtcattaa gtaaaagttc ttttccagtt gtcaaatcta tattgtaatt attcaatgta
    20821 ttgtatttag gcccatagtt attagcaaac cccataaggt ttaatatcat actcagtaca
    20881 taatttttat taaaagtaac tgcgtaactg gtagataccc aatagctttg ttttggaagt
    20941 gaattttgag aagctgcgtt attatattct cgttcttctt cctgtaagcc atttttaaaa
    21001 ttatttacag tcattcttac agcattgttt atgttattta aaataactgg gtttacatac
    21061 gtggattgat ttctaatatt gtatataaca ggatacttta catcatatga aaaatactta
    21121 cttctttcaa aatcttcatt aaattgtaca tataatatat tatatgtaca ggtatttgta
    21181 gattgataat tttccattaa aatcctcccc cttttataaa aaaatcagat acaatataat
    21241 atgcttaaaa cagtaataat gttaatagta aatgtaaaga atagaaagta tctaaaacag
    21301 caaaaaatga ctaattttgt ggtataatac ataggattga gagttatttt ataggaggaa
    21361 aaatatgtta ttattagcag ataaatggaa agattatgaa cttatagata tgggaaatgg
    21421 agaaaaatta gaacgttggg gaagttatgt acttagaaga ccagacccac aggttgtatg
    21481 gccaatggaa tctgaatggg ctttatggaa aaaccctcat ggacattatc acagaagcaa
    21541 taaaggtgga ggtcaatggg aacacaaaaa aagatatcca gaacaatgga ctataaatta
    21601 taaaaactta aagtttcaca ttaaaccaac tggctttaaa catactggat tattcccaga
    21661 gcaagcagct aactgggatt ggatgataga gaaaataaag aaagctaaca gacctataaa
    21721 agtgttgaat ctttttgcat atacaggtgg agctactgta gcatgtgcat ctgctggtgc
    21781 agaagtatgt catgttgatg ctgcaaaagg tatggttaat tgggcaaaag aaaatataga
    21841 attatcaggt cttaaaaatc aaacagtaag atttatagtg gatgatgttg taaagtttgt
    21901 tgaaagagaa ataagaagag gtaaaaaata tgatgcaata ataatggacc ctccttctta
    21961 tggaagaggt cctaaaggag aagtatggca aatagaagaa aagttataca gttttgtaga
    22021 tttatgtatg ggagttttat cagataaccc tttatttttc ttaattaatt cttatacaac
    22081 aggattttct ccaatagttc ttgaaaatgt attgtctaca acaataggta aaaagacaaa
    22141 tacaggagat atatacggtg gggaattagg aataccagct tccagagatg gtaagatact
    22201 tccttgtggt atatttggaa gatgggagaa aaaataatga taaaagtcat atatgaagat
    22261 aaccatttat tagttgtaga aaaaccagtt aacatattgt ctcaaggaga taatactaat
    22321 gataaagata tggtaaatct tttaaaagac tatattaaaa aaaagtataa caaacctgga
    22381 aatgtatata ttggtttaat acatagattg gatagaccag taggtggagt tatggtgttt
    22441 gcaaaaacat caaaagcagc atctagattg tctgagcagg taagaagtaa gactttcaaa
    22501 aaaacttatc tagctgttgt tcatggcaag ataaaatcta actcagatat actaaaagat
    22561 tttttatata aaaataaaaa aactaatatg gtaactgtag tgaaaaaaag ccataaagat
    22621 gccaaagaag ctgaacttaa ctataatgta ttagatttta aagaaaatct aagtttagtt
    22681 caagttaatt taaagactgg tcgttctcat caaataagag ttcaatttgc aagcagaaaa
    22741 catccattat acggagacca aagatatggc aaagaaataa ataagattgg acaacaaata
    22801 tctctttggt ccaataagat agaaatttgc catcctacta caaaagaaaa aatggaattt
    22861 acatgtcatc cacctgacaa atatccatgg aatatatttt aaatctcaaa tgaggtgatt
    22921 atatgagtaa aaaattttca ataggagaaa tgtctaaact tcataaaata agcatacaga
    22981 cactaaggta ctatgaccaa atagatttat ttaaacctgt tcatgttgac caagaaagca
    23041 attacagata ttactcaatt gaccaatttt cacaattaga tattataaaa tatttaaaat
    23101 atttaggtat gcctttaaaa gaaattaagt caaagcttga tgaaaataat gaagaaattc
    23161 taaaattact aacaaataaa attaatatag tggatgaaaa aattaaagaa ttagagctta
    23221 taaagaaggt actaagcaca aagaaagaaa ctatggaaaa aggtatgaaa gtagatgaag
    23281 ttggtaaaat agtgagaaaa catattccac gaagaagtat cttatctata gagtatgaag
    23341 aaggattaag tagtgaagaa actatggaac tttccagaag acaaatagca aatatattag
    23401 aagataatat atctgtgttc tatggtggag taagtggact tgtatctata gataaatttt
    23461 taaaagaaga taaagttatt tatgacaata gttttgttac tgttgaaaga gacttatttg
    23521 atataaaatc acaaaaacat ataacagaaa tttcatctgg tgattttatt tgtatgacat
    23581 ataaagggtc ttacaaagaa aattataccg cttttaagaa actggttgat tatgttgaat
    23641 ataaaagaat acctattgaa aattatatat atgaaatacc tataatagac cctctgtcta
    23701 caaacaacga agaggaactt ctaacagaaa ttcaaataca agttaaaaag agagaaattg
    23761 acttaagatg tctagtgtag taatctagtt tacttacaca tcttaatata gttgtctatt
    23821 atcaacatat gatttttcta gatagtgatt cctaacagtg agattttcaa aaaataggag
    23881 gaaatatggc taatgctaat tccctcctat ttttaattaa tccatatcat tttaattaag
    23941 ccatgtcatc acgaagtgca tcaataatat tttctttttt tatcttatta atagtataca
    24001 acattgtaat aaatactata aataaaacac taaatatact aattccaata ctaaaccatg
    24061 gaatcacaaa atcaatatca tctgtaacca ttactttatg aattagccat gaagaaatta
    24121 ctgctatagg aagtccaaag aacaatgctc tcatgccata aaaagcacac tcaaaattca
    24181 tcatcttttg gaaatcatgc tcagacattc ctacagagcg aagcatggca agttctctac
    24241 gacgtaactt aatattagtt gaaattgtat taaacacatt ggcaatcgaa atcagagaaa
    24301 tcataattat gaaagtatag gcaaaaacat tggcaataaa aatgtaatta cggttctcat
    24361 ccatcattcc atacatattg tacagattgt actcattagt aactccctca ctctcaataa
    24421 tttgctccat ttctgcaact gactgagtag gatttttcga ttgaaagctt aaactttcaa
    24481 ttcctgtaga aacaccagga atctcaaact tatctttggc tgaataagga gccaatactc
    24541 tgaagcttgc tttcttcaca tctgaacttc tgattcctgg aggagaatca ggtggcacta
    24601 ctttgacaaa tgaaatattc acattttgtc tctcaggtat ctccaacttg ccattagttt
    24661 caggagtgag actaacattc atagaagaac tgttgaaaat atcaataata ttattatcct
    24721 ttgttaccat agaaacagca atcagttttg cattttgtcc agtatattct tctgctggta
    24781 atccaagact tttaataata ttcagataag ttctatcatc aagaaattgc atatctactg
    24841 gtaattcaag catttcactc atagaatgtg aaccctcata gttcaaataa tcgtctgtaa
    24901 gctcacttac tttggcaaca catgaatagg tcataagttt ctgatacaaa ccttcgtaaa
    24961 caccatttgc agtttttagc ttatcgtaaa gcttcaacat ttcgttgtct ttcatacctt
    25021 gtgtagaaaa aataatatcc tgatcagtaa cctctgacct ctgatttgac atctgtttta
    25081 ggtctgctac taaagcactt gtagatataa ataaaactat gcttaaaaca agcgacaata
    25141 caatacttcg ataacgtttc ttgtttcttt taaagttttt catagcaaga actccctcaa
    25201 gaccataaac tcgctgtgtt agttttgatg tcttcacaga tttagattca atttttacct
    25261 catttgtctg acgaatacac tccatcactg gtgtgttggc agcctttctt gctggtatgt
    25321 aagcagaaat taagattgta accatactta ccactgctgc aacgatgatt gcagggatag
    25381 acaatgttaa tgtcaacgga acgtttacat agagaatatt tgcaaagttt ttagctatga
    25441 cagaaatcac aagtccaata ctgactatac cgattgcaac accaattggt ataccaatcg
    25501 caccaatgca aattccttca aatagcaccg aatttcgtaa ctgttttgct gtagctccaa
    25561 cagatgaaag tattccaaat tgatgtgttc gctcgttcaa tgacatgtta aatgaattat
    25621 atattagaaa aattgagcct agcatgatta tggcaagcac aatgccacca accgagtaca
    25681 aaaacatatt gaacaaattg ttatcagaaa cacctatgaa gcgcaacaca ttgtcattga
    25741 aaatggaagc atggtcttta gtgctacttg tgtaagagcg aatctgatgt ggatttttca
    25801 atgtgacaaa aaggctaaga ctgtcagtct tgacttgttt atcttctttg gttatcaagg
    25861 tatatcctgg tgcagaagat tcttcaaata cgggctttct acagataccc acaactgtat
    25921 aagttttctt atcctgtggc acaaaaactt cactcccagc agtgtagggg tcactctgac
    25981 caagcttttc attttcactc attcgtttcc ctacatccaa agaaatagta tcacctactg
    26041 caaatttgac accaccatct gtcgcaactt tacttgaaac aagaatctct ctactatttt
    26101 caggtaatct tccagaaatt aaagttatag gcaaagtatc aaaagcttct ttgctaaatc
    26161 cagcaataaa aagatatggt ttatctggat ttctgttacc ttcaagtgtt gcatagccaa
    26221 tattttcaaa tgttgcagta tttgcaactt catcattgac tctttgttcc tgtacaaaag
    26281 aagaatccac atccaaaaac tggacatgcc aatcaccata ttttataata gctccatttg
    26341 tcatatagtt catcaaagaa actccaaagg tagtaactgc tgtaatcata gcagctgaca
    26401 gaacaactcc tataattgtt acaagagttc gagtgcgatt ctttttcaat ccttgcagag
    26461 caactttgtt aaaaatgttc attaacgatt caccttcctc tcatcacgca ccacttttcc
    26521 atctgaaata cagataattc tatctgcctg taaagcaatg ttttcatcat gtgtgacaat
    26581 aatgagagtt tggtgatatt tactatggct ctctttcagt aactggataa tctcttgtcc
    26641 atttttactg tctaagctac ctgtaggctc atcagcaagc ataactgctg gtgcattcat
    26701 caatgcacga ccaatggcaa cacgttgctg ttggccccca gaaagttgat ttggtaaatg
    26761 tgtttttcgt tcttttaaac caagtaattc taacaagtca tttaaccgtt cttgattgac
    26821 cttacgctta tccataagta taggcaatgt gatattttcc actacattta atgtgggtat
    26881 cagattgtga aactgataaa tcaatccaac ttgtcgccta cgaaaaatag caagtttttc
    26941 attgctttgg ccatatacat cttgcccctc cagatatatc tttccacttg ttggtacgtc
    27001 tactcctgca atggaatgta gtagtgtaga tttaccagaa ccagaagaac caatgattgc
    27061 agtaaactcc cctttttcaa tagtaagtga gacatggtca agtgcagtaa ccttattttc
    27121 attcttacca taaaccttac ataaatcttc aatctttaaa aacgccatta tataagtctc
    27181 ctttcatatt ttataaatat atcatagaac tttcaactta catttaagtg acatttaaat
    27241 gagagattcg tcactttgga aaacgaataa caaatattgc accaccttgt gggtgatttt
    27301 gtgcagtaat tgttcctcct tgtctcataa taatcgtctt acaaagtgcc aatcctattc
    27361 cataaccagc tgtgcttgaa tttttcccac gataaaatct gttaaataga catggtaagt
    27421 cttctttttt aaagcctgct ccgttgtcat gaatagtaag ttgagtaaac aaaaagttgt
    27481 cctcacaaac gatatcaatt tttccattat cacccacact ctccatgcag tttttaaata
    27541 tattttgaat tgcttctgaa agccaattta aatcacccaa aatccttact ccttttggta
    27601 tatcaatatg taaagtgata ttgtgcagtt ccatgggaat caaaagttga tgaagtgcag
    27661 aactaatcaa attattcaca tcaatttgtt ctttttgaaa gactacaata cctgcatcta
    27721 aacgtgataa ttttaaaagg gaagtaatca gccagtccat tcgtataagt aattcctctg
    27781 tttctcgtat caactctttt ctctcatctt catcagaagt attctctaac agtgacaaaa
    27841 taagagttac tgatgtaaga ggagtgcgaa gctggtgggc tatgtcagct agtgagtcag
    27901 caaggtgttc tttttctttt ttcagtgcgt aattttgctc tctaatacgt aacgtcattt
    27961 tagttatttc actttgtaga atagaaagct caccctcatc tgattcagca atatacagat
    28021 ggttagcatt atgaagcaca agattaattt gttctgaaat ctgtgcaata cttttgtatc
    28081 gatttttagt aaacacaaaa aatgctattc caaaggtagt ggcagaaaca atagaaagta
    28141 ttccagtcat aatactgatt gcaaatccca gggctacagt gagaatagta attaataaga
    28201 ataaaatagc aaattttcga aactctctat ttcgaaacat aacttccccc caatctatac
    28261 cctgtcccac gaacagtcaa aattatttgt ggacttgaag ggtcatcttc tatcttctct
    28321 cgcaagcgtt tgatgtacac agtcaatgtg ttgtcattaa caaactcacc tgccgcatcc
    28381 cacaattcat caagtaatct taccctcgta ataatacttt taggattgct aataaacacc
    28441 aataacaaac ggtattctaa agctgaaaga aaaacctcat tgccattctt tttcacaaca
    28501 ccggttgccg tatctacata aagcccacaa atttcaaaag ttgacccaaa acgcccactt
    28561 tttcgtagtg ctgttctaat tcgcgcaatc agttcacgtg gacgaaaagg tttagtaata
    28621 tagtcatctg cacccatatt cagtccagta acaacacttg attcatcacc agaagccgtc
    28681 aaaaagataa caggaatgtc ttgcttttct ttgatttctg tgcaaatcgt aaacccattt
    28741 ccatcaggta aagaaatatc aataagtgcc aaatcaaatt tattcttagc aatctctaca
    28801 agtgcttcac ttcttgtgga tacatgggtt actataaatc cttctgaatt aagcaaaaga
    28861 ataagatttt tggcaattgc tttatcatcc tcaactaaaa atatccgttt catttacttt
    28921 catcctcctt tattttatta gttttatata ttccagcaga gcaatagtac actactattt
    28981 tacttatcat tacacaattt tcagtagttt cttttgttct tatataatcc atgcataata
    29041 cctccattga tttattatat ttcttataac ataaatacac aagataagac aaaaaacata
    29101 atgtaagtgc aagtttttta actcactaac ttataaacat acactcctac taacactact
    29161 ttcttgatga cacatcttac aagcttatat tcatacatca atgaaaaatt aaatttatcc
    29221 atttattaaa ataaaaggca atagcttcta tagctaatgc cctttatctt aaaatgattt
    29281 aaatactata caatttttat aaatggctat aaataaactc gtatagaata taaaatttat
    29341 ctacagtctt ttgttataca aaatttataa cctcttatag aacaaattta ccactttgta
    29401 aacttatcac ttttactcag ctatattata aacttttaat ttatctttgt ttaaatgttt
    29461 cttcttaact tctactaaag cttttagcgt atccaaagat gtcattattt cagtagaaaa
    29521 ttcaatggct gttcttctca tcttaaatcc atctcttgta gagtcatttc ctttagttgg
    29581 tgtatttatt actatatcaa cttgtttatt tcttatagca tctaatatgt ttggtctagg
    29641 ctcttcaatt c
//
LOCUS       DAMKPG010000043        29641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMKPG010000043 DAMKPG010000000
VERSION     DAMKPG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.046"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            1081..2454
                     /locus_tag="PC621_002018"
     CDS             1081..2454
                     /locus_tag="PC621_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109177.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            2710..3777
                     /locus_tag="PC621_002019"
     CDS             2710..3777
                     /locus_tag="PC621_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF4109178.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3874..4182
                     /locus_tag="PC621_002020"
     CDS             3874..4182
                     /locus_tag="PC621_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF4109179.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            4175..5605
                     /locus_tag="PC621_002021"
     CDS             4175..5605
                     /locus_tag="PC621_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF4109180.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            5683..6057
                     /locus_tag="PC621_002022"
     CDS             5683..6057
                     /locus_tag="PC621_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF4109181.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            6051..8255
                     /locus_tag="PC621_002023"
     CDS             6051..8255
                     /locus_tag="PC621_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF4109182.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            8255..9664
                     /locus_tag="PC621_002024"
     CDS             8255..9664
                     /locus_tag="PC621_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF4109183.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            9649..10713
                     /locus_tag="PC621_002025"
     CDS             9649..10713
                     /locus_tag="PC621_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF4109184.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10743..12164
                     /gene="nhaC"
                     /locus_tag="PC621_002026"
     CDS             10743..12164
                     /gene="nhaC"
                     /locus_tag="PC621_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF4109185.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            12593..13057
                     /locus_tag="PC621_002027"
     CDS             12593..13057
                     /locus_tag="PC621_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF4109186.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            13355..13717
                     /locus_tag="PC621_002028"
     CDS             13355..13717
                     /locus_tag="PC621_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4109187.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            14312..15172
                     /locus_tag="PC621_002029"
     CDS             14312..15172
                     /locus_tag="PC621_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109188.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            15430..17544
                     /locus_tag="PC621_002030"
     CDS             15430..17544
                     /locus_tag="PC621_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4109189.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(17806..20049)
                     /locus_tag="PC621_002031"
     CDS             complement(17806..20049)
                     /locus_tag="PC621_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF4109190.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            20381..21097
                     /locus_tag="PC621_002032"
     CDS             20381..21097
                     /locus_tag="PC621_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109191.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            21125..22063
                     /gene="blaCDD"
                     /locus_tag="PC621_002033"
     CDS             21125..22063
                     /gene="blaCDD"
                     /locus_tag="PC621_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF4109192.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            22407..23090
                     /locus_tag="PC621_002034"
     CDS             22407..23090
                     /locus_tag="PC621_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109193.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            23087..25747
                     /locus_tag="PC621_002035"
     CDS             23087..25747
                     /locus_tag="PC621_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109194.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(25928..27109)
                     /locus_tag="PC621_002036"
     CDS             complement(25928..27109)
                     /locus_tag="PC621_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109195.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(27109..27771)
                     /locus_tag="PC621_002037"
     CDS             complement(27109..27771)
                     /locus_tag="PC621_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109196.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(27916..28458)
                     /locus_tag="PC621_002038"
                     /pseudo
     CDS             complement(27916..28458)
                     /locus_tag="PC621_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(28576..29641)
                     /locus_tag="PC621_002039"
                     /pseudo
     CDS             complement(28576..29641)
                     /locus_tag="PC621_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        11280 a         3571 c         5002 g         9788 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agatgactaa agattacata tactattact
      481 taaattaaaa atttaatata atttataatg tatacaattt attataaaaa atttaatttt
      541 aattttctat ttttgatact ataatatata atcttgataa tttaattaaa aatctagatt
      601 ttacatacca aactggtttc ttatttcgct atctttactt atgaactcat atattcctat
      661 aaaggttatt ttagaaaaag ttggttattt aaacattaat accacaattt ctatagattg
      721 tttgttgaat aatctataat tatattatgg actaaaaaat ccaatgagtt aatttattgt
      781 ttttttatat ttgagagatt acaacttatt agtttgtata tctctttttt taataaaatc
      841 aataatatat aaaactattg gcatatttaa tgacaattca aatattgtat ataaccataa
      901 tgaaagatat ataaataaaa ttttaaatat acttaaaaat atattaatat aatgtacata
      961 aaaaatatca taaatatatg ataagataaa tacaatagca atattttatt aattttttgg
     1021 gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt ttttaaaata
     1081 atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa agacgggaaa
     1141 actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca cgatatttta
     1201 gccaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac actattagaa
     1261 gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga caaaaatgga
     1321 aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga gtttgtaggg
     1381 gcacttgaaa tatcaaagaa tatgacaacg atagaaaatt tactaaacag aaattataaa
     1441 aaaaatttag tcaataaagt tagtagtaga caaaaaaagg gttatgaatt tgatgatatt
     1501 ttaggggaaa gtaatttgat taaagaagca atagaaaaat ctaagaaagc ttgcaagtct
     1561 gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt tagccagtcc
     1621 attcattatg gaagtaatag gagaaactat ccatttatag cacaaaattg tgcggctttg
     1681 ccggaatcac tattcgaagg tatccttttt ggaactagca agggaggatt tacaggagca
     1741 atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt ggatgagata
     1801 aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga aggttatgta
     1861 agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc aacgacaaat
     1921 gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata ttataggctt
     1981 aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat acttctattg
     2041 tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt aggaatttca
     2101 gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag agagctagaa
     2161 aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga aacaagtatg
     2221 attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa ttattcttta
     2281 aacgatttgg agggcaaaac acttagtaag attatcagtg agatagaaga aaagtatata
     2341 tatgaatcat tagaaaaggc atcgtacaat ataactaaag ctgcatcaat tttaggtatg
     2401 aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt ataaataagc
     2461 aagataaaaa aacaaaatag aagtaaaata aaaaagcaaa atataggagc aaaattatta
     2521 taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt attttttatt
     2581 tatgaaaata cataataaaa taattatagt ttcaacgttc caataaatat ttactttaat
     2641 tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa tttttaggag
     2701 ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat gggaataggt
     2761 atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg tagtagaagt
     2821 acttcaggga aaagtatgta tgatgtttta ggaattgaaa gaggagaaag accagaagtt
     2881 attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt tgttttgtta
     2941 gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt aaatagaaaa
     3001 ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga tgcagattta
     3061 gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg aacaggaata
     3121 aatccaggat ttgtattaga tttactagta ttggcacttt caggtacttg tgaagaagtt
     3181 actagtataa aagctaagag agtaaatgat ttatctccat ttggaaaatc tgttatggag
     3241 gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga taagactata
     3301 gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt aggttggaaa
     3361 ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag aaaatctaaa
     3421 tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata tggatatgta
     3481 aatggagacg ttttaattga aatggagcat ccacaacaga taataccaga agtagaaggt
     3541 attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt acaaataaat
     3601 cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat tccattaata
     3661 ataaatgcta gaccaggtct taaaactatg ttggatatac cagtgcctag agcaataatg
     3721 ggagacataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc taattagtaa
     3781 gataacagtt agctaattgg taaaataatg tttaatttaa aataacagta aattaattag
     3841 taaggaatag tcaattaata aggggatgaa aaaatggatg ctaaaattaa tgattgggtt
     3901 attatacaca atatagtatt gactccagaa gaaagagctc cacaagttcc agaggatact
     3961 aagaaagtat ccttagaaat gtgggttaaa ggttttatac aaagtgatgc ttctataggc
     4021 gacttagtag aagttaaaac aattacaggt agacttgtaa aaggtaattt acttaaagta
     4081 aatccatatt atactcatga ttatggtaaa tgtataccag agttactcca aataggaatc
     4141 caagctaaag aaatattatt tggaggtgta tataatgaat aattcaacat caataaaaga
     4201 tatgagttat caagctgtta tgggaagaaa taacgaaata atgaaaaatg caatcggatt
     4261 ggattattct tcttttgaac aagagggaat agggtttgat tatgagaaaa tgatgagtga
     4321 aacaggatat acattacaag atattgaagc aattcaatca cagtatgctg ttggaaatac
     4381 tcctctaata gagcttaaaa atttgactaa attagctaga aaatgtgcta aagaaggtaa
     4441 aggagctaaa atatttgtaa aagatgaagc aatgaatgca tctggtagtt ttaaagcaag
     4501 aagagcagct actgcagtat atcatgccaa acaaatggga tataaaggtg taatagcagc
     4561 taccagtggt aactatggtg cagctgtagc atcacaagcg gcaatgcagg gattaaaatg
     4621 tataatagtt caagaatgtt atgattctaa tggtgtaggt caacctgaaa ttatagaaaa
     4681 agcacgaaaa tgtgaggcat atggagcaga agttgttcag ttaactgttg gaccagagtt
     4741 attttatacg ttcttagttt tattagaaga aacaggctat tttaacgcat cattatattc
     4801 accatttgga attgcaggtg tagaaacttt aggttatgag ttagctatcc aatttagaga
     4861 aaagtataaa aaagaccctg atatagttgt ttgtacaaat gcaggtggtg gtaatcttac
     4921 tggaactgca agaggattga taaaagctgg ttccattaat actaaagtag taggagcaag
     4981 tgtagattta aaaggattac acatggctag tgataatcaa tttaataaaa aatcatttac
     5041 aacaggacat acaggttttg ggataccata ctgtacatgg cctgataggt cagatgtacc
     5101 taggtctgca gctagaccac ttagatatat ggatagatat gtattagtaa aacaaggaga
     5161 agttttttat acaactgaat tattggcaca gctagaaggt atagaaagag gtccagcagg
     5221 aaatacatct ttagcagcag cattctcttt agctcaagag ttagatgagg ataaagttat
     5281 agtggtacaa gaaacagaat atacaggcgc aggtaaacat atatgtccac agcttacttt
     5341 tgcaagagaa aatggaatag atataaaatt tggaaatcca agagaagaaa ttgcaggagt
     5401 aaacttaata ttaccagaaa gaccagaatt attgaaatgt gttgatattg atatgaataa
     5461 aataagaaag tcatttataa aaaattgcat atctaataat cacattgata atattgataa
     5521 gctaagtaat cgagatatag aatttttaat ggaagaagta aaaagttcaa gagattttgt
     5581 tgttgatgta ttaaataatt tatagtaatt attgtttttt tatttaatat aaaataaaaa
     5641 ttcggaaaat tctattaaat caatgcgaat ggaggaatgt tcatgaaaaa aagagaggat
     5701 gattttgaag ttagaagaaa acatcttcaa gaactgagtg atgatgagtt aaaagaaagg
     5761 ttttggaatt tagctaccca gatagtagaa ccaatgatag aacttggtaa aaagaataca
     5821 acaccatcaa tagaaaggtc tgtcttgttg agaatgggat tttcttctct tgaagtaaaa
     5881 cctattttag aaggggttat ggaaagaggt cttataggta aaggagcagg gcatgtagtt
     5941 tataaattgg ctaaatctaa aaatataacg gttagagaag caggtttgtt attagttaag
     6001 ggtgaatatt gggatgaagt tacatgttta tttagagaag gggtagaatc atgttaaagg
     6061 aaaataaaaa attggatata gactatatat taaaagattt ggataaatat aaaccaaaac
     6121 gaagaggttg ggtttggaga gaacacttgg aaaatcttga aatgggacct tttaaatata
     6181 aagactgtac aaaaccttta aagaagagtg tagggctacc atcatctaag tatttcaata
     6241 atatagaccc tcaaccaagt ccagttatta ctactgaaat agcttctggt agatttgagg
     6301 atgatataag aagaatgaga atggcagctc atcatggagc tgaccatcta atggttatca
     6361 gaactgctgg acaatcacat tttgatgggc ttatagaagg aactccacaa ggtataggtg
     6421 gagttccaat aacaagaaaa caagtaagag ctcaaaggaa ggctcttgat tttatagaag
     6481 aagaagttgg tagacctatt aattaccatt catatgtaag tggtgttgct ggtcctgaag
     6541 tagcagtaat gtttgctgag gaaggagtca atggagcaca tcaagaccca caatataatg
     6601 ttctgtatag gaatataaac atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa
     6661 taatggcttt tgccaatata gctcaaattg atggagcaca taatgctaat gctactgcta
     6721 gagaagcatg gaaggtaatg cctgaactta tggttcaaca cgctttaaac tctatattct
     6781 cagaaaaaat aggtatagat aaaagtaata tatgcttatc aacagttcca ccaacagcac
     6841 ctccagcacc atgtttaaga atagacttac cttacgctgt tgcacttaga gaattattta
     6901 gtgattataa gatgagagct caaatgaata ctaaatatat ggaatcatca actagagaag
     6961 caacagttac tcatgtatta aacttattaa catcagtatt aactagagct gacatacaat
     7021 caactataac acctgatgaa ggtagaaatg taccttggca tatatacaat atagaagcat
     7081 gtgatacagc aaagcaagct ttagttggta tggatgggct tatggatatg atagaactta
     7141 aagatgtcgg tgaattaaga gacaaagcaa gagaacttaa agaaagagct gtattatata
     7201 tggaagaaat atctgaagtt ggtggatatt ttgaatctgt ggaacaagga ttttttgttg
     7261 attctggaaa ttatccagaa agaaatggtg atggaatatc tagaaaaata aaaggtggag
     7321 ttggtgaagg tactgtttat gaaagagaag aagattattt agcacctgta actgctcact
     7381 ttggatacaa taatgtagct caatatgatg aaaatgctat agataatcca tcaatgttaa
     7441 tagatggatg tacatttgaa aatccagaca aaataatata tattgatgag cttgacgatt
     7501 ttgataatgt agaaaataga ctaaaagaat catatgagta cagaaatgga actaaaatta
     7561 agcctgaaat ggaatggtgt gcagatggaa ttgtaatgat aacaatgatg cttcctactg
     7621 ataaaagaac agcagagttt gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa
     7681 ttgaggtcat aagtagagaa gttatgcatg aatctgaagg gactagaata gaagtgaaag
     7741 gaagagttcc gtttgatata gatttaaaca atttagtaat accagaagaa cctaaggtac
     7801 taactgatga agaaataaga gcagatatag aagaaaaacc tatgaaaata gtttcagcta
     7861 cggttggaga agatgaacat tcagttggtt taagagaaat aatagatata aaacatggag
     7921 gtattgaaaa atatggcata gaatgccact atctagggac ttctgtacct gtagaaaaat
     7981 tggtagatgc agcgattgaa ctgaatgcag atgcaatact tgcatctaca ataataagtc
     8041 atgatgatat tcactataaa aacatgaaaa aattacacga ttattgtgta gaaaaaggta
     8101 taagagataa agtaatgatt gcgtgtggag gaacacaagt aacacctgaa atagctgttg
     8161 aacaaggcat cgatgctgga tttggaagaa acagcaaagg catacatgta gccactttct
     8221 tagttgaaaa aagaagagaa atgagtaata aataatggaa aatagcaaag tttctttaaa
     8281 aatagatgtg ttggtagctg aaattggaag tactactaca gtggtaaacg ctttccacga
     8341 tattaatact aacaatccta tatttttggg tcaaggtcaa gctccaacaa cagtgtttga
     8401 aggtggcgat gtaagaaatg gcttatcagg tgctattaaa gatttagcta ataaattatc
     8461 tgtagatgat atagagtata atgatatgtt tgcaacttct agtgcagctg gtggattaaa
     8521 aatgactgtt catggattag tctatgatat gacagtaaaa gcagctaaag aagcagcttt
     8581 aggggctgga gctgttattc gtcaaataac atcaggtaga attaaaagaa ctgatttaaa
     8641 taaaataaaa gaaataaacc caaacataat tttaatagct ggtggagtcg attatggaga
     8701 aagagataca gctatttata atgcagaaat gatagcttct atgaatctag gcataccagt
     8761 tatatatgct ggtaatgttg aaaatcaaga ggaaataagg ttaatatttg aagatactaa
     8821 ttataagctt tatataactg aaaatgtata ccctaaaata gatttattga atatagaacc
     8881 tacaagaaga atcattcaaa gtgtatttga agaacacata actacagcac caggaatgaa
     8941 atacattaaa gaaatggtta atgaaaatat tactccaacc ccaggagctg taatggaagc
     9001 ttctaaactt ttatataaaa atattggaga tttattaact ttggatgtag gtggagcaac
     9061 tactgatgta cattctgtta cagatggaag tgactatatt aataaaattt tggttaatcc
     9121 tgaacctact gcaaagagaa gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa
     9181 tatagtagaa gttataggta aagaaaactt acaaagtgaa ctatatatag atattgatgc
     9241 agtaatagag aattatcctc caataccaaa atcaaaagaa gaaatattat ttgttgagag
     9301 gttgacaaaa gaagctgttg ttaaagctat gctaagacat tcaggaaaaa taagaaatat
     9361 ctatggaaca actggaaaag tcaaaattgc agaagggaaa gatttaacag aagtaagata
     9421 tatagttggt acagggggag cacttactag gctccctagt agaattgaaa tattagataa
     9481 tatgctcaaa tacaataaaa ataatgaatt actatttcca aaggaaaaaa caaagatatt
     9541 aatagataat gactatatta tggcatctat gggtgtttta tctaaaaaat atgaagaagc
     9601 atctcttaag ctcctgttaa aaagtttgaa ttttgaggag gaaagattat gtatcctagg
     9661 ttagaaatag atatagaaaa gttaaagcat aatgcaaaat taatttcgca gatgtgtcat
     9721 gaaagaagta ttaagatatc ttttgttact aaatcatttt gtgcacaaaa agaaatagta
     9781 gaagaaatat gtagtgaagg aatagaccat atagcagatt caagaataca aaacttaaaa
     9841 aaattacaag atataaatct acctaagata ttgattagaa tcccaatgtt aagtgaatta
     9901 gaagaagtta tcaattattg cgatattagt tttaactcag aattagaaac aataaagaaa
     9961 ttaaatgaat tatgtgaaag taaaaatatt attcataaga tagttcttat gtttgactta
    10021 ggcgatttac gagaaggata cttttatgaa gaagactttt ttaataatgt aagagagata
    10081 gtaagattaa aaaatattga aataattggt atagctacaa acttaacctg ttatggagct
    10141 attataccat ctagggaaaa tataggcaga ctggtaagta tagcaaaaag aatggaagaa
    10201 aaatttggaa taaatctaga aatagtatct ggaggtaatt caagttctat tcatctgttg
    10261 ataaataata atatgccaga aggtattact aatttgagaa taggcgagtc cattctactt
    10321 ggtagagaaa cggcatacgg tgaaaatata aatggaacat atcaagatgc atttaaatta
    10381 atatgccaag tagttgaatg taaagaaaaa ccatcagttc ctattggaga aataggagtt
    10441 gatgccttta gaaataaacc tgtttatgaa gataagggaa tcttaaaaag ggcaatcata
    10501 gctataggta aacaagatat aaatattgac tctttaattc caatagacac tgatataaaa
    10561 atattagggg caagttcaga ccacatgata ctagatgtaa gtaacacaaa atatgattat
    10621 aaattaggtg acaatttaga atttttatta acttatgggg gtatcatgtc atctagtaca
    10681 agcaaatatg tttcaaaaaa aattatagtg tagtgtttaa ataattagga ggaaactatt
    10741 ttatgaaaga gcgagaatgt agaaaagcaa gtctgtttga tgcaatgata ccaattactt
    10801 gtttgatatt atttttaagt ctaggagtat tagtgtatgg ttcatcacct catgtaccac
    10861 taataggagc agcagcagta gcaggacttg tggctgtata tagacttgga tttaaatgga
    10921 aagagttaga attaagtatg tttaatagta taaagatggc aatgcaagca atcttaatca
    10981 taattataat aggcgtactt ataggaactt ggatagtaag tggagtagtt ccatgtatga
    11041 tttactgggg actaaaaatt ttatcaccaa atattttttt agtagcatca acattagtct
    11101 gtgcaatagt atctcttgcg acaggttcat cttggagcac aatgggtaca gtaggagtag
    11161 ctcttttagg aattggtcaa agtttaggta tgccaattgg attaatcgta ggttctataa
    11221 tatctggagc ttactttgga gataaactat cacctttatc tgaaacgact aacttagctc
    11281 cagcaatggc aggaacagat ttgtttacac atataaaata tatgttatat tcgactatac
    11341 catcattatt aatttgttta gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag
    11401 cactagatat aaaacaaata gaattaatta ggtctacatt agattctaca tttaatactt
    11461 tatcaccagt attattatta gctccagcaa tagtaattgg attagttgta tttaaagttc
    11521 ctgctatacc agggcttata gtaggtgctg tattgggagt attgtttgca gtagtttttc
    11581 aaggagaatc tatgaatacg atattagatg cagcacaaaa tggatatgtt tcgtcaacac
    11641 aaatacagga ggtagatgct ttattatcta aaggtgggat tatgaatatg atgtccactg
    11701 tatccttaac aatatgcgct ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg
    11761 aagtagtagc atcttcttta ctaagattag caaagggtgt atttggaaca gtattatgta
    11821 ctatggtaac ttgtacaatt accaatataa tagcaggtga acaatatcta tctatagtta
    11881 taccaggaag aatgtataat aaggaatata aaaagagagg tatacatcct aagatgttgt
    11941 cgagagcttt agaagatagc ggaactttaa cttctccatt agtaccatgg aatacatgcg
    12001 gggcgtatat aacagcaaca ttaggtgtat ctgcactaac atatggtcca tacgctttat
    12061 taaatattat aaatccaata gtatctttag tattaattgc atgtaagttt aaaatagcaa
    12121 gaatagaaga tgaacctgaa acttgtttaa attttgatgt ttgataatag ataacttatg
    12181 taagaacgta ggaactaggg ggggagcctg tggggaatta gtaagacata ttttagtgtg
    12241 cgaaattcta taaatcagta atcttgtgac tttagccatg ggaggttcag attagtaaat
    12301 ttatagtttt atattagtct atactttgat cttttaattg tttgtataaa ttttttatag
    12361 aaaaagattt cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt
    12421 ataagagcac tgtgtgacaa gaatgatgag attatttttg ctatactttc gatataagaa
    12481 atggaaggag gttatgtttt atatttatat gaaataatta gttacacagt gaattttcgt
    12541 ataatttggt ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag
    12601 aatatccaat ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac
    12661 aattgacttt atggtagagt tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa
    12721 atatctggag gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat
    12781 ggatttagca aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa
    12841 aaatgagatt ctacaaagga ttgttcctgc acagaaaata ataactgcaa ttgatttagg
    12901 accttatgaa ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg
    12961 atataaactt catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag
    13021 tgaatatctt acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt
    13081 agttgataat tagaagaaca tttaatgcta ctgcataaat tttatcctaa ataatatcaa
    13141 ggtgatttaa tttatgtatt ctaatgtaac tgtagataat caatttataa ctaacttagt
    13201 gtaatttaga tttaatttat tattctaatg taaccccaat atattgttaa ggtctactta
    13261 agatgctcaa aaggctatgc ttaatttatt gttatataat ttgtagagtg gtataattta
    13321 gaagtgatac tatatagata tataggggga aaatatgaaa atatatgata tattaacacg
    13381 tttttatatt caagatatag aaaaagccat acctttttat gagaatcttt taaaagaaaa
    13441 atgttctttg cgtttttctt ataaagaagt tgggttggaa ttagcgcaaa taggaaatgt
    13501 tttactatta tctggttctg atgatgctct taaaccattt atagaaacaa aatctacttt
    13561 tatggttgat tctgttgatg aatggagaag ttatctattg gataatggag cagttgtagt
    13621 aagagataag aaaaaagttc ctacaggata taatatgaca ttgagacatc cagatggtac
    13681 aattatagaa tatgttcaac atacaaagca ggattagtta aaaatagatt ttactactga
    13741 gcaactaatt agaatctatt cttagacgta caatttatta tttttggtat taatagatta
    13801 taagtcaaat tataatattt caaaatagaa tagaaattta tttacataaa agagagtgtt
    13861 taaaaatgaa ctttttagtt cacgaagcac tcctttttgt atgaaatcaa atacatcaat
    13921 atataaaaca aactgatgaa gagcttaaat taaaataaaa agattcataa caggacccca
    13981 ttaaacttta agatagggta agtttgtaat catattaaat aattgtaaaa agctgttttt
    14041 agtcgtataa tataaatatt tatcatttaa ttaaggaatc taaaaggaat ctaataagaa
    14101 gaaaataata aataactttt tagatttact aaatgaaaaa ttattgaaaa ttaaaggatt
    14161 agagtcttta atcagaaagt aaaaagtaat atattaaatt tatgaattga aaacttaaac
    14221 aacaaatttg tttataaaat agcaagattc attaaatata actaagtaat aattgatata
    14281 atgttgcatg gataaaagga gggaagctgc aatgtcttac aaagtaatta atgtcataac
    14341 tgctattgac tatatagagg agcatttatc agaaaagctg gatttagata taattgctaa
    14401 tgctgttcat tactcaaaat atcacctgca tagaactttc acaacttctg ttggtctaac
    14461 aatgcatgat tacataaagc ggagaaagtt gacagaagca gcaaaattat tggtattttc
    14521 aaagaaacca attattgaaa ttgcattgat agctggatat gaaagtcaac aggcattcac
    14581 ttctatattt aaagctatgt ataaaaaatc tccaaataaa tataggaaag aacaagaatt
    14641 ttatcctttg cagctaagat ttgttttgaa aaaagataac ctattttcaa agaatgtact
    14701 agagttggaa aaagaaatta aacttgcaag tatgtctgac ataccattat ggatgaattt
    14761 agtgagatta gtcatagatg gatttccaaa tttgcaagag gaagaatata tatatcaatt
    14821 agaacagtat attttagaag aaagagcttt aatattaaaa cttaataatg tcgctattgc
    14881 aaatatggtg tttaatagag aaactagaag tattgatttt tttggaatac atccacagta
    14941 tcgtaattca gacattgcac aagtgttttt gaagaaagtt attgaagatt tcttgataga
    15001 tacagatatc tcaattacta cctttcgaga gggtgacaaa gctgatacag gacatagaga
    15061 tatgataaag aagttggggt ttgcagaagc agagttgctt gtggaatttg gatatcctac
    15121 tcaaaaattt attcttcctt gtgtagaaga aagagataat agacaaaatt aattgtagat
    15181 taaatgcaaa gtttaccaca tattttttgt ttgaattatt ttatatataa gagtatttct
    15241 gcttttaatg tattttgctt gtaagtgtac ttatggtaat aaatatagaa agatttagtt
    15301 ttttaaaaaa agaactttaa agctaaaaag ctaacattta agttaaatag caagaatgat
    15361 taaaaactcc aaaacacaat taagtataat gattcagtgt aagtacttaa ttaacaaagg
    15421 aggatttaaa tgtctgaaaa taaacaagta gtaaattgcg atattattgc atccaattct
    15481 aaacataata tgtgtgaaca tgatgtagag ttattaactt tttttccaga tgagattgca
    15541 catctaatag agataaataa caagctagac aatgctttta agaaagctga aaatcttgtt
    15601 gacaagcttg ataaggatta tatggacgct aaaatgtata tggtaaaaaa tcgtggtgaa
    15661 atagacccac atgaaatgtt tcaaaatgaa caaggactaa aacaaataga taactatggg
    15721 gcttttatgg taaaagttcg agataagatt aataaaatca aagattctcc atactttgct
    15781 agaatagatt ttaggctgaa agatatggat gatgaatcta agtattatat tggtagattt
    15841 gcatttgatt atgaagatga gcttataata cttgactggc gttctccaat tgccagtatg
    15901 ttttatgatt atgaaatcgg aaaagctgga tatgatgctc caataggatg ggtggatgga
    15961 gaaataacaa gaaaacgaca atttaaaatt aaaaatggaa aattggaata tgcactagaa
    16021 agttccatta atatacaaga tgatattctt caaaaagaac ttagtcacac ttcagatgaa
    16081 aaaatgaagt caatcatttc aacaatacaa aaggaacaaa atcaaattat tagaaatgat
    16141 aaagctgata cactgattat tcaaggggta gctggttctg ggaaaacatc tatagcatta
    16201 cacagaattg cttttcttct ttatcgattt aaagataaaa tatcagcaaa taatgtaatt
    16261 atcctatcac ctaataaagt ctttggagac tatatttcca atgtccttcc tgagcttgga
    16321 gaagaaccat tatgtgaatt aagttttgaa aatatagcag aggtacaatt agatagagtt
    16381 attaattttg aaagtgagaa agacccatta gaaataaatg atgctaaatg gtctgaaaga
    16441 gtgcgtttta aatctacact tgattttgta aaacttattg atgattatat aaaacaaatg
    16501 ccaaataaga tttttattcc aaaagactac acttttggaa gttttacagc taaaagtgac
    16561 tggatacaga gtagatttga ggcatataac agatatccag ttaagaagag attagaaaag
    16621 gtagctgaag acattcatta taaatttgaa tcagataata ttatggaaga agatttacca
    16681 aaggtaaaaa gtatacttaa aagtttgaat ggaatgttga caataaaaaa tactcttaca
    16741 ctatataagg atttctttaa acaaatgaat atatcaaaca tgtttgtgat ggcagaaaag
    16801 aaaacacttg aatggtcaga tgtataccca tttatatata ttcatgctgc atatgaaggt
    16861 atacaagaag ataagattat aaggcatatt gttattgatg aaatgcagga ctatacacct
    16921 attcaatatg ctgtaataaa cttacttttt aagtgtaaaa agactattct tggagatttt
    16981 ggacaattgg ttaatcctaa tcatactcat acattagatg atatgaaaca gctatataat
    17041 gatggggaac tagttatgtt gaacaagagt taccgttcta cttttgagat tattaatttc
    17101 gccaaaaaga ttcaagatat atcttcatta gagcctattg aacgacatgg tgaagaaccg
    17161 gctttattaa aatgtaataa cgagcaagat gaaataaata agataaaaat agaaattgaa
    17221 gaatttaaaa agagtgataa tgcgactctt ggtattatat taaagacaga taatgacgct
    17281 aaagctgtat atgatgcgtt aagagaagaa tatagtgttc acttaatatc ttctgaaagt
    17341 tctagcttta caaaaggagt atccataact tctattaaaa tgtcaaaagg tttagagttt
    17401 gatgaagtta ttgtaccttc agtaaataat aaaacatatt atagtgatta tgaccgcagt
    17461 ttgttatata ttgcttgtac acgagctatg cacaaactta agttaacata tgttggtaca
    17521 ttaactcaac ttataagcat gtagattata tcttatatgc atttattatc gtaaatataa
    17581 attgggacaa agattcatca ataaatcaac aaaaaataat acttataaat ttataaaaaa
    17641 tgaaagctat aaattacatt tgaaatttta taaaattttg tgatttatag cttttataat
    17701 attaatttct tttaaaataa agttaaactt taaatttatg aaataaaaca aagtagtaat
    17761 atatatgtaa tagttcttaa ttttttaata ttagtattat attatctata tatttattga
    17821 ttttctaagg aaatctgatg ttatagtgca acctctttca aacatttcta gtggtgtacc
    17881 ttcaaatacg acctgaccac cgtttttgcc accatcagga ccaatatcaa ttacccagtc
    17941 tgcttgtttc aatacatcca aattatgttc aattataatt acagtattac ctttatcaac
    18001 tatatgctca aaaatcaaca taagcttttc aatatcagaa ccatgaagcc ctgtagttgg
    18061 ctcatctaaa atatatatag tacctttttt gttgaggttt ttagctaact taattcgttg
    18121 acgttctcct ccagatagtg tgctaagtgg ttgacctaag gtcatataag gtaaaccaac
    18181 atctttcatt gtttgtaaga ttttacatag cttcttttct gtaaaaaatt caagagattc
    18241 atctactgtc atagatagta catcaactat acatttacct ttatataatt ttgacaaaac
    18301 ttcttcacta tatctagtac ctttgcaagc ttcacatgta ttaataatag ggtccatgaa
    18361 tacaagctct gtaacaagta ctcctttacc atcacaaaca ggacaagctc cctttgaatt
    18421 aaagctgaat aaaccttctt caacattatt ttctttagca aatacttttc ttatctcatc
    18481 aaagaaacca agaaatgtag caggcataga acgatttgta gctgtaactg ggctttggtc
    18541 gattttaata acatcttttt cgtatttttg tgcaaaaaca cgagagatta aagtactctt
    18601 gccagaacct gctactccag taacgacagt cataattcca actggtaaat ctacatcaat
    18661 atgttttaga ttatttatgc aagcatctct aattggaagg gtaccttttg gatgacgaga
    18721 gtcagttttt ataggtaatg atagattcat tgcatttgaa gtaagagtgt ttgctaaaag
    18781 taatttatca taagagcctt ggaatacaac atgtccaccc tgagtaccag cacttgggcc
    18841 aatatctata acttcatctg ctatggagat aacatcttta tcatgctcta ctacaaggac
    18901 agtatttcct ctatctctaa gtttttttaa tagctgattc atacgatgta catctcgtgg
    18961 atgcatacca gcactaggtt catcgaaaat ataagtcata cctgtgagac tgctccccat
    19021 atagcgtaca agttttaatc tttgagcttc tcctccagat agagatgatg attctctatt
    19081 tagatgtagg taagggagac caatatcaat cattcttttt aaaccatcag taagagtctt
    19141 tattacagtt tcagttcttt tatcttttat ttgtaacaat aaatcataaa ggtcagtaag
    19201 ttccatattg cacatatctg caatcgaata tcctaaaata ttacagttaa gagtttcttt
    19261 atttaatctt tttccatgac atacaggaca ttccttatct gttataagtc tttctgattt
    19321 ttcttttgtg tgtttgctca tattgctaat atctcgattt agatatgttt gattgtattt
    19381 gttaaaaata ccctctattt ttggattttc ttgttttcca tctttatttt ttgaaccata
    19441 aagcaataaa ttatattctt caaaagaata atcttttata ggtttatcta aatcaaaaag
    19501 tccagactct gcatactgct tccagtacca tgaattaggt ttaaataaag agtcatttat
    19561 acatcctgta ttccatgaat tttcaggatt tataatagat tcaatattga ttactttaac
    19621 tctaccaatc ccagaacaat ttttacacat accatttggg tcattaaaag aaaaatatga
    19681 agttgttcct atgtagggtt ctccaattct agaaaataat aatcttaatg atgaatacaa
    19741 atcacttata gtgccaactg tagaacgtgc attgccacca agaggagttt ggtctacgat
    19801 tacagaagca gttaaattat caatcagctc cacctgaggt tttctatatt taggtagcct
    19861 atttctgata aatgaagaat atgtttcatt catctgcctt tgtgattctg ctgcaattgt
    19921 atcaaatacg atactggatt ttccagaacc agaaactcca gtaaaaacaa caattttatt
    19981 tttaggaatt ttaaatgata tattttttag gttattttgt gtaagtcctt ttattataat
    20041 atttgacatg aatgttaaat cctttctgag tacattttag taaagcaatt atattatgta
    20101 accatattat aataaaatta aaatctactt tcttgatata ttctgctatg ttatagaagg
    20161 tatttctaaa tttaaccaat cccatacaat ctgaataaat gatagttgat aatagataaa
    20221 aaagtatata atagatactc gtaatttgat aagtacaaag ataattatac aattaaaacg
    20281 acttgacatt taaaacttac tattgtaata tttaaatatt acaatagtaa gttttaaata
    20341 gagaataaat aattacctca caaactgaaa ggaggacata atgatactaa tagttatatt
    20401 gagtatattc atcatagcct caatcaacat aattatatta ggtaaagaaa atataagaga
    20461 tatagttatc cctttaactc tatttattgt aagtgatttt ttatttttgt gtggaaatat
    20521 aatatgtagg ggaggagtaa tgctactatt tataggttta tttgcaatac cattgatgct
    20581 aattgcattt atatggattt gtgtacatat gattaagctt tactataaaa aagatttaaa
    20641 taaaagaaaa atgattggat gtatatttag tatctttata agtataggta ctatatttat
    20701 tcctagtcca agtgaaagtt ttagatttaa actttatgaa aaagattata gtgtagtagc
    20761 taaagctatt ttgaaagttt atgatgaaaa aaagttaagc tctgaatctg tcttttatat
    20821 tcaatctgat aaaaatgaat tggagaagat attttctaaa gaagtaataa aaaaaatgaa
    20881 aaaactaaac agaaatttag atgtagttaa aatttctggg aatagaaatt tagtctattt
    20941 tttctttgga gctgaattac aatctattga tggtattgtg atttttaaag atttaaaaca
    21001 tgttaaacca aagttagatt ttataaatag aagtattcaa gctcctagat tggagtatat
    21061 tacaaataat gtatattatt ttaaaggtga actataaact tatgtagagg agatggtata
    21121 aagtatgaaa agaaaaaata attttatttg gatagttatt cttctagttg ttgtagctat
    21181 gggagtatat tatgtaggaa gaaagcataa caacacaaaa caaaaacatg ataagaatca
    21241 tataaactct gaattaaata aatccaaagt tgaaaacaat gataaaaaca aaaaagaaaa
    21301 aattaatatg gttgactata gtgactgttt tgaaggtatt agtggaggag ctatttttta
    21361 taatactaaa aataaagaat ataatatata taacaaagaa ttaattgaaa caagaaggtc
    21421 accatgttct acttttaaaa tagtttcaac tttaattggt ttagaaaaag gggtaataaa
    21481 ctcaaaagaa tcagttatgg gatatgatgg tacagagtat ccaaataaaa attggaataa
    21541 aaatcttagt ttagaagagg catttaagga gtcttgtgtt tggtactaca aaaaattgat
    21601 agataaagtt gatgctaaat ctgtccaaaa cattttagat gatttaaagt atgggaattg
    21661 cgatattagc gaatgggaag gagatttaaa aaatggaaag ggacatttaa atggattttg
    21721 gttagaatct tcattacaaa tatctccaaa agaacaagtg caaacaatgg caaaaatatt
    21781 tgagggagat acaaacttta aaaaagagca tattaatatt ttaagagata ttatgaaaat
    21841 agatgtaaat gataaaaaca taaatgtata tggaaaaact ggaacaggat ttgatgaaaa
    21901 aaataaacgt gtggatgcat ggtttgttgg tatgctagag cgtgaaggtg acacctatta
    21961 ttttgctatt aaatcagatg attccaataa agaaattaca gggccaaagg taaaggaaat
    22021 tgcaattaat ataattaaga aatattatag tgtaagggaa taaacttaat tttatttgta
    22081 aatattattt acctatagtc tgtaatattt attaaataat tttactaaat tgagtctata
    22141 tagagcaaaa ctcctttagt acataaaata cttgctattg attattaaat tgaataccta
    22201 tagatttaaa gttaatacct tttaaagact atgtaatata ttaaaagtat aaataaaata
    22261 tattgcatag tttttgtttt atattataat tttaaataat gtgcaaaaac taaacaatat
    22321 ttttaatagt aatttatatt tgtattcatg tcactataat gtcattttag gttgctaaaa
    22381 tttaaccata aaggagatga ttgtatatgg aaatattaaa atgtgaaaat ctcacaaaaa
    22441 tatatggttc aaatcaaacc agagtgacag cccttaataa tgtaaattta tcagttcaaa
    22501 aaggtgattt tgtatccatt gtaggagcat ctggttctgg caaatctaca ttgttacatc
    22561 tgcttggggg agtagataga cctacatctg gaaagattta tgttgaagat acagaaatat
    22621 caagtttaaa ggaagaagct cttgcagttt ttagacgtag aaaagtaggt cttatttatc
    22681 aattttataa tcttatacct acccttgatg ttagaaaaaa tatattatta cccatgttac
    22741 ttgataaaag aaaggtggat gaggatagat tctctgagat tgtatctata ttgggattat
    22801 ctgatagatt aaaccatctt ccaagtcaac tttcaggtgg acaacaacaa agggtatcta
    22861 tagctagaag tttaatctat agaccagcta ttttattagc agatgagcca actggtaatc
    22921 ttgatagaaa aaattcagaa gaaattgtag acttgttaaa cttatcaaat aaacgattta
    22981 atcagacaat acttcttatt actcatgatg agaaaatagc actagaggca aatcgtattg
    23041 taactatgga agatggagtg attgtctctg aaaaggtggt gaagaaatga atattcttca
    23101 agaatacacc ttagactttg tacgacgcaa taaacgtagt agtatagcta taatgttagc
    23161 tatattactt actacaacca tgatgtcttc tttgtgtgga ctgttgtata ccatgtggac
    23221 agattttata agactttcaa ttgagaaaga aggtaactgg catggtgaat tgtttgacta
    23281 tacttatgga agtgacttac cattaattga aaatttttct tcagttgatg atgtaatggt
    23341 aaaaggacaa tggtatgtag gcaaaattaa tgatgagaaa agagattata taatttatag
    23401 aaatgctaca tcagagtatt ggaattctat gccagaaaag gatactataa tagagggtcg
    23461 tcgaccaaat aatgctaatg aaattgcatt atcaaaacaa tattttgaga ataatccaag
    23521 tgtaaaaatt ggggataaaa ttactcttcc aataggagat agaatcctaa atggaaagcc
    23581 tcttgaaccc atagctcaaa aatcgaaagg ggaaagtttt aaaaaaattt cagagactac
    23641 attgacaata gtaggaaagg tagatgtttc cacttcatca gtaatacctg catatacagc
    23701 tcttggatat cttgatagaa caagtataaa acctaatgat agtataactg tgtacttgcg
    23761 atttaaaaat ataagagaca catacaagga acttccaaaa cttgctaaat cattggggtg
    23821 gaagactaat gaatatggaa aatacaatct taaatataat agtaattatc ttttaaaaat
    23881 gttagtcttt tccccagaac agaaagcatc tatgagcagt atatcaaaat tttcaactcc
    23941 tattatgtat cttacaatag caatttttac agtagctgtg tttgtaatgg tgatttacaa
    24001 cgcgttttca ctatctgcaa atgctagact tactcaatta gggatattat ctagtgttgg
    24061 tgcatcacca aaacagatta aaatgtctgt agtgtttgaa ggttttttgc ttacaattat
    24121 accattgcca ataggtttat ttttaggatg gttattgtgt aataggctta tagtttatat
    24181 taattcagtt aattatcata ctgatgttcc agaagtggta tttacttatg gtattcctgc
    24241 ttttctgcca gcagttttac ttacaatagt aacagtctgg atttctgcac ttataccagc
    24301 acgaaaagta tcaaagatat ctcctataga agcaatacga caaggagata gtgttaaaat
    24361 caaaaagtcg cataaatatt cattaggaaa gatatttgga atagaaggag aacttgcatc
    24421 aaacgcacta cgtgctcgta aaaaatctta tcgtactgca acaatatctt taacattatc
    24481 ttttttatta cttacatgtt ttttgcatat aattgcaaat caagaaggag caaaagctgt
    24541 ttatggaagt gataattata aggaccaaag agatatagca gtttatttgg aaaatagtga
    24601 acctgtagag tctgctttca ttgaaaaatt agataataca gaaggtgtaa aaagttcact
    24661 atattatcaa aaaatgtctg cttccacttg gctgactgaa aaagatgcat caaaagaact
    24721 tgcgaaaaat ggaggattta aaagtatagt ttcatctaaa aaatactctc ctatagaacg
    24781 tgatggcaag tttcgtataa tttcaaatat aattgggctt gatgataaga gttttacaga
    24841 atattgtagg caaatttctg tagacccaaa gttattttat gatacaaaaa atccaacctc
    24901 aattgtatat aataaggagg aagatattaa tcgtagtaca cgtagaaata aagtatatat
    24961 agattattta aatctttcaa taggagataa cattaaatta aatgaaaaag tatatgacga
    25021 agataaaggg gattatactt acaacatgaa agtgggagct attacaagca cacttcctaa
    25081 aacagtagat actagttcaa aatatacatt aatgcaaata gtgccaatgt ctatggtgca
    25141 ggaaatatct aaaaattatg atgaagtaag taggttacgt tcaaataggc tgataggagt
    25201 atttaaagta aataatcgtg aggacattcc tagagtaaga gaaaaattag agtccattta
    25261 taataaagac tatggttcag gagattatag tattgaggat gttttagaaa gtgaaaaaca
    25321 aaaggctagt ggaataaaga ccatgaattt aatagtaggc tttattacag gtttattggc
    25381 tatgattgga ttatcaaatg tacttgctac agtgtcagga aatactagaa gtagacgaca
    25441 agagtttgct atgttgcgtt ctgttggatt atctccagaa ggcataaaaa agatgcttgt
    25501 tttggaaggt ttgttcttag gaattacacc attactactt agtattcctg tgcaaatagg
    25561 aatagtgtat gcttttctac gtataaatga aatatatttt atagagtatt taccatttgc
    25621 acctatatct acaataatag gttttactat attgatactt tttattgtaa tagcatcata
    25681 tatgacagga tacaagcagt tgaagaatga aaatatagta gaatctataa aaaatgaaac
    25741 aatataaaca aaagctattt gaacttattt aaaggtttcg atttaagact acttacaagt
    25801 atagggatag ttaatttgtt tgtttaataa aaccttaaat gtaaatagaa gtatattact
    25861 aaaacaatag ataacataga atatagatat agctggatta taggtatttt acctataatc
    25921 cagcatttta tgtgtaaaat ttaattatga atcttgcacc accattatgg caattttctg
    25981 catgtagagt accattttga cgttctataa tagactttga tagagcaagc cctataccaa
    26041 tactgtcctt actagcattt ttaccacgat aaaatctatt aaaaatatat ggaagttctt
    26101 cctgagcaaa ccctttacca ttatcttcta tgataatctc aacatataga ggattttgac
    26161 tataataagc aactatatat ccaccatctt ctatatgctc actacaattt ttaagaatat
    26221 ttaaaagagc ttctaaagtc caattaatat cgatttcaaa ttctaccgaa ggagaagcct
    26281 cgatagtaaa tgtctgatgt ttttctctta aagaaccttc tataggctca attgcataag
    26341 taagaagaga atgtacatct ataagtttgc tttcaaatat taaagtactt gcatccaatt
    26401 ttgataatgt aagtaaagat gaaactagtc tctcaagtct tgagatttga tttgtaagtc
    26461 tgttaatata atctatttct tcatctggac aattttctga aagtaattgt gccataagtg
    26521 acatagaagt aattggagtc ttaagttgat gagcaatatc tgcaagatta tctgaaagag
    26581 tttgtctttc ttttaaagca aattcttttg aacaacgaag ttccccaact gttttatata
    26641 tctcatcttc aaggtgagaa aattcatctt cacatcgtaa aagtacagac tcttttccta
    26701 aattcacttc ttctaagtat tgagtaagat tgtttattct actcagtttt tgcttatttg
    26761 ttctatgttt gattatatat acaactgtag ccattgttga aattatacca aacgatatca
    26821 ctagaaaata aaaaaagttt ttactccaaa atgtactttt agaatatcca tgttttgata
    26881 aaatttcatt tcctgtttta aaatccttat cactagaatt tttcagtgat tttagtatac
    26941 tttctgtaga cttaggattg tactcatatg atgcaccaag agccttagac agtacattgt
    27001 attcattaaa agaagttata gttgttagaa ttacacttaa taaaaaagtt ataattaagc
    27061 agaatattat aaaaacaact gtactgttga tatttcttgg agttttcact agctgtcctc
    27121 cattctatat cctatgcctc ttaatgtttt tatacaaggt ggatttttta atttttctct
    27181 aagacgcttt attgtaacag taagagtgtt atcatttacg aaattaccat ctacatccca
    27241 gagcatttct agtaactttg tacgggtcat agctctattt ttattttcca taagacttag
    27301 aagaagttta tattctcctg ctgtcaaaag tacttcaaca ttatctaaat atgctttgac
    27361 ttcaacttta ttcaagctta tatttccaca tgtaatagta ttgttagtat ttgaatgagt
    27421 tcttctaaga actgcattaa ttctggattg aagtactgag agcagaaatg gtttaaccac
    27481 atagtcatct gcacccatat ctaatccacg tataatgtct ttttcatcat cacggactgt
    27541 caaaaatatc actggagtat cttctatact tttaacatat tggcaaaact cgtatccaat
    27601 accatcaggt agatttaaat caagcagaat taaatcaata gttttatcaa aatttttttt
    27661 agcttcagca atcgtattat aacaacatac agtgtgattt ttcttttcaa gggaagtttt
    27721 tattccaaat gtaattgtat catcatcttc taaaagaagt attttagcca tttactttta
    27781 gtcctttcag taatttagtt atttcattaa ctaagttatt agttttatgt ttaatatttt
    27841 ttatgttaga gatagaagtt agagatagaa aaaattatca tttaagtata taaactttaa
    27901 tttataaact taggtttatg ctttaattga atctattaaa agagcaagag ttgtttcaat
    27961 aaatttttcc tcatctatat taaagttgtt caggtatgac tttcctgtta cagatagtaa
    28021 attaaaaaat cctgtaagtg taaatattga tgaatacatg agagtaggta tttcaatatc
    28081 atgcctaata cttctatcat tttttccaat ttcaaacatt ccttgtattt catcaaacat
    28141 aaatttatta aaagaaaaaa acttttcttt aaacagcata tcagtatccg atttttcaga
    28201 gtttacaata ccaatcattc ccataaggca taatagagaa ggttctacag agtaaaattt
    28261 atgaaaagca aagtatgaaa gttttatttt ttcaaaccct gttttgcctt ttgcactttc
    28321 tattttaaca ttatcccaaa gtcttttata tcctcttaag acaacagcaa agaataaatc
    28381 ttctttacat gaaaagtact tgtatatggt gcgtttagta tattcaactt cttttgccaa
    28441 ctcattcata gttgtattct gaacctccca tgactaaagt cacagggttc ctgcttcata
    28501 gaattttgca tactaaaata tgtcttacta attctccaca ggccatcccc gtagttccta
    28561 cggttcttac ataagttatt tattttgaat cgttattagt cttaatcctt cttttaatat
    28621 atttatactt gcatttatat ctctatcgtg agtttcatta caactaggac aaatccattc
    28681 tcttatattt aagttcttaa cttcctcgtt tttataccca catttattgc atatttgtga
    28741 acttgcaaag aatttaccta ctttatacaa tttgtctacc ataccaatta gccttgtatt
    28801 ctaattgacg aataaattct gaccatgata catcagaaat taagcgagat agtttatgat
    28861 ttttaatcat attctttact tgtaaatctt ctatgcaaat aatatcattc tcttttatta
    28921 atttagtaga taatttttgt agaaaatcat ttctttgatt agctatatgt tcttgaagtc
    28981 ttgcaacttt taatcttgct ttatttctat ttaaactacc gattgtcttt ctcgataatt
    29041 ctctttgtaa tttagcaagt ttgtttaatg acttcttaag atacttagga ttttctataa
    29101 attctccaca acttgagata caaaattctt tgattcctaa atctaaacct gtctgattgt
    29161 tagtattttc aaatacttca atatctacat cagtacagca taatgataca taatatctac
    29221 cacttggttc tttagatata gtagcattaa gtattctacc ttgaggtact tgtttatccc
    29281 ttatcttaac cataccaagt ttaggtagtt ttatatgttg accacaatac ataatgttcc
    29341 catttgtaaa gttagtttta tatgaaaatc tattaatttt ctttgattta aatttaggaa
    29401 atcctgcctt ttctttaaag aatttttcat atgcgttttc taaatctttt aaggcgtttt
    29461 gtagtgaaaa tttgtcaggt tctttaagcc actttaattc tttttttaag ttggtcaaat
    29521 cagaactaca ctgtttataa gtaaaagttt ctttatcgtt tttataaact tctattcttt
    29581 tagcaagata cttattatat acaaatctac aacatccaaa agttttgtta attaactctt
    29641 g
//
LOCUS       DAMKPG010000044        29193 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMKPG010000044 DAMKPG010000000
VERSION     DAMKPG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.047"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     repeat_region   717..1670
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=717..745
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            2887..3042
                     /locus_tag="PC621_002040"
     CDS             2887..3042
                     /locus_tag="PC621_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF4109197.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     gene            3288..3539
                     /locus_tag="PC621_002041"
     CDS             3288..3539
                     /locus_tag="PC621_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109198.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(4748..5131)
                     /locus_tag="PC621_002042"
     CDS             complement(4748..5131)
                     /locus_tag="PC621_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109199.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            5258..5602
                     /locus_tag="PC621_002043"
     CDS             5258..5602
                     /locus_tag="PC621_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109200.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            5785..6000
                     /locus_tag="PC621_002044"
     CDS             5785..6000
                     /locus_tag="PC621_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109201.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(6986..7303)
                     /locus_tag="PC621_002045"
     CDS             complement(6986..7303)
                     /locus_tag="PC621_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109202.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            complement(7595..8164)
                     /locus_tag="PC621_002046"
     CDS             complement(7595..8164)
                     /locus_tag="PC621_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109203.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
     gene            8608..8998
                     /locus_tag="PC621_002047"
                     /pseudo
     CDS             8608..8998
                     /locus_tag="PC621_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            9318..9827
                     /gene="vanZ1"
                     /locus_tag="PC621_002048"
     CDS             9318..9827
                     /gene="vanZ1"
                     /locus_tag="PC621_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF4109204.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(9929..10123)
                     /locus_tag="PC621_002049"
     CDS             complement(9929..10123)
                     /locus_tag="PC621_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF4109205.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
     gene            complement(10638..11030)
                     /locus_tag="PC621_002050"
     CDS             complement(10638..11030)
                     /locus_tag="PC621_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4109206.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            11598..11960
                     /locus_tag="PC621_002051"
     CDS             11598..11960
                     /locus_tag="PC621_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109207.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            11944..12660
                     /locus_tag="PC621_002052"
     CDS             11944..12660
                     /locus_tag="PC621_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109208.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            12680..13099
                     /locus_tag="PC621_002053"
     CDS             12680..13099
                     /locus_tag="PC621_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109209.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            13089..13613
                     /locus_tag="PC621_002054"
     CDS             13089..13613
                     /locus_tag="PC621_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109210.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            13925..14482
                     /gene="efp"
                     /locus_tag="PC621_002055"
     CDS             13925..14482
                     /gene="efp"
                     /locus_tag="PC621_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF4109211.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            14574..15029
                     /locus_tag="PC621_002056"
     CDS             14574..15029
                     /locus_tag="PC621_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF4109212.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            15192..15902
                     /locus_tag="PC621_002057"
     CDS             15192..15902
                     /locus_tag="PC621_002057"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF4109213.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            15956..17026
                     /locus_tag="PC621_002058"
     CDS             15956..17026
                     /locus_tag="PC621_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4109214.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            17054..20608
                     /gene="smc"
                     /locus_tag="PC621_002059"
     CDS             17054..20608
                     /gene="smc"
                     /locus_tag="PC621_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF4109215.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIGSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            20635..21915
                     /gene="ftsY"
                     /locus_tag="PC621_002060"
     CDS             20635..21915
                     /gene="ftsY"
                     /locus_tag="PC621_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF4109216.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            22018..22386
                     /locus_tag="PC621_002061"
     CDS             22018..22386
                     /locus_tag="PC621_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF4109217.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            22383..23735
                     /gene="ffh"
                     /locus_tag="PC621_002062"
     CDS             22383..23735
                     /gene="ffh"
                     /locus_tag="PC621_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF4109218.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            23773..24045
                     /gene="rpsP"
                     /locus_tag="PC621_002063"
     CDS             23773..24045
                     /gene="rpsP"
                     /locus_tag="PC621_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF4109219.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            24110..24337
                     /locus_tag="PC621_002064"
     CDS             24110..24337
                     /locus_tag="PC621_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF4109220.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            24445..24960
                     /gene="rimM"
                     /locus_tag="PC621_002065"
     CDS             24445..24960
                     /gene="rimM"
                     /locus_tag="PC621_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF4109221.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            24969..25664
                     /gene="trmD"
                     /locus_tag="PC621_002066"
     CDS             24969..25664
                     /gene="trmD"
                     /locus_tag="PC621_002066"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF4109222.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            25849..26199
                     /gene="rplS"
                     /locus_tag="PC621_002067"
     CDS             25849..26199
                     /gene="rplS"
                     /locus_tag="PC621_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF4109223.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            26520..27416
                     /gene="ylqF"
                     /locus_tag="PC621_002068"
     CDS             26520..27416
                     /gene="ylqF"
                     /locus_tag="PC621_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF4109224.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            27805..29097
                     /gene="brnQ"
                     /locus_tag="PC621_002069"
     CDS             27805..29097
                     /gene="brnQ"
                     /locus_tag="PC621_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF4109225.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        12002 a         2851 c         4852 g         9488 t
ORIGIN      
        1 cacaaagagt gacttacgag ttgaatttaa atttatattt aaatccaaaa aataaactct
       61 gtttatgatg aactccactc taacgcaaaa tagattgtag ttcttcttgc ttttattata
      121 ccacaaaaca ttataaataa aactgttgtt tataaaaaaa tgcaaagtaa aattttttat
      181 aaacaatgat aaaaatttta ttatagagct attaacttat gaaatatctg taaatgagta
      241 gatatttcat aagttaatat ttattgtttg tataaaatat ttggtaaaaa taagtaagtt
      301 ttatatgtta taataattgt agcaagaata ataatcgaaa gcagcaagca tgtcagctgg
      361 tgttatatca gtttatattt atgataaaat aaaaatctcc caaacgccaa taaggatgat
      421 ttaaaaaaat aatattttca cttaaaaata atcactcttt ataggagtaa attattttct
      481 tgattttatt atactacaaa ttggtacaga tatttaaaaa taatatattc ataatattat
      541 aaaattataa atagttttgc agtgagcgat atttttgata aaatagggcc taacagttga
      601 aatataaggc attgagggta tgtgataaat gttatcattt gcactactca tggttcactg
      661 caaatttgag agaattgtat agatgtaagt gttggaaata ctcaatttat tttggggttt
      721 tatattaact aagtggtatg taaagtctcc atctccaatt aacataaata attcatccgc
      781 tggttttata ttaactaagt ggtatgtaaa gccgcttggc aaatcataag aaggattatt
      841 ttctccaacg ttttatatta actaagtggt atgtaaagaa aataaaagag tttgaattac
      901 ccattttttc atggttttat attaactaag tggtatgtaa agtacgacat tgaataaaat
      961 gggtacagag acaatttata agttttatat taactaagtg gtatgtaaag tttataaatc
     1021 ttttaatgac tgtctctgat ggtacaggtt ttatattaac taagtggtat gtaaagaaag
     1081 gaatagatga tatgattgct aatgctgaac aacaagtttt atattaacta agtggtatgt
     1141 aaaggggtct gactttaata tatatatata tataaattgt tttatattaa ctaagtggta
     1201 tgtaaagtta taaactccca cgacaaaatt aactataacc tcgtgtttta tattaactaa
     1261 gtggtatgta aagagtttta aagagggcag acctaaaaaa tatactaaaa gttttatatt
     1321 aactaagtgg tatgtaaagg cagaagattt agcgactgtt gttgctgaat gggatggttt
     1381 tatattaact aagtggtatg taaagattaa attgctcagt ctagtactta aagaattata
     1441 aggttttata ttaactaagt ggtatgtaaa gttgctatga tttaattata cgacgttttg
     1501 tctttaaagg ttttatatta actaagtggt atgtaaagtg ctgtgccaca atttgatgct
     1561 tctatagcta aattcgtttt atattaacta agtggtatgt aaagctttta agtgcatcaa
     1621 aggagttaca agctatattt agttttatat taactaagtg gtatgtaaag acagatctaa
     1681 tatcaaaata aggttatgac ttttaaaagc atagccatat cagtaaaaat ttaattatca
     1741 tttaaaaaat aaatttttat ttaaagaata cacataaata tttccagact taactctctt
     1801 aaaccatttt catatccttt agtagatact tttgttattt tttaatcttt agtaacttat
     1861 tcttgagtga atctcttatt ttttctaagc tatttcaagc ttctcaaaaa attctatatt
     1921 tatattcatc acacatataa tacaatcaat tcagttttaa ttgccaatat ttagtttttc
     1981 tgtatctgat aaaccaagaa cataatcagt atataaacca aaaattttag caaatattat
     2041 taacccatcg tctcttgttg gtctttcacc agattctatt cataacactt gtatttatat
     2101 ttgttttttc aagcaattct ttttgagaac tattcatatt ttccctagta tatttaattc
     2161 tttgatttac atctatacat aattacctcg aaaaatttct gttttggaaa tattgttatt
     2221 gaaatatagc aattaaggtg aatatatgaa aaagatacac tatgatttta taaaaaagaa
     2281 gacatacata aaatttatac ttacaaagtg ttgcaaaaga gttaggtttt aaaaatgcat
     2341 caacttattt taagtacagg aaggggtaat attcaattag agttgatatg ttacctataa
     2401 actatctaag atacttgatt atgatataga aaattttttt acaaattaaa tttctaagat
     2461 agaaatttaa ttaagaggat gatgaggatg aataatttac aagtaatata aagaaataat
     2521 aagagaattt taaatgcata gcaactagca gatatatatg aaaatgatgt aaacaatata
     2581 taaactaact tcaatagaaa taaagagaat gtaaattatg ttttattaca attcgagtat
     2641 ttgaaagaat ttaagaacta acctactaat agtcagttga tttcaaaaca ttctagtcaa
     2701 ttataccttt ggatcgaaag atgtgcaaat agacactgca aaatattaga tattgataaa
     2761 acttgggaac aatttgacaa tttagagaaa atatatttca agattaagcg agagaaacta
     2821 agctgtatag aagatgtatt aatagaaaat ttaagattta aggatacaaa ttaataaagc
     2881 aataatatgg cattagatgt aaaaatagaa gttaaaataa taaaagatgt tgttatatta
     2941 aatataatac attggaaaaa agaaacaagt ctattaacag ttaaaattgc tagaggtcaa
     3001 ggtgatttaa gttatgtaaa atattttact aatgagagct aagagcttct taacaagagt
     3061 tttggtgtgt ctcttgaaac aatgttaaca attaataaaa gacgaatggc agaagaaggc
     3121 gtatgtaagt ttaaaagaga taagttaaac aaactagata ctatataaga ggataagaaa
     3181 cttatagaag aatatgttac ttttgtaaag gatttagctt taaaatatag aatataaagt
     3241 gattttaagt aaaaattgta acaattaaat aagtataaga caagtttatg agttcatatt
     3301 ttataacaag gggtgatgtt atgaaaatac tacaacaatt taaaccatac aaatatgata
     3361 aagaaaaaaa tatagaatat gatgaaaaaa ttatagctaa tcaaaacaca gaagtttata
     3421 tagtatatcc acgtatatct gatgaagaaa ataggagaag atgggaagag ttcaatgaag
     3481 tagctaaaga agtagctatt aatatagcta tgaagaaagt tgagacagta gaaagttaag
     3541 agaatagaga agttgctaat acttagcact ttctcataga agaaaattgg acaactattt
     3601 ttaaataatt aggaataaaa aagttaaatc atatttgaaa aaaggcgata aaattgttga
     3661 gtattgttat tagtaataag aatgtattaa aagaaattat aagtttttag taaatatagt
     3721 atttaaggat attaaaactg gtaaaaaata tcatttagac tattaaataa aaaagtgctg
     3781 gtcaaagtaa gcaacacaat aaataaaaaa acttatagaa attataagat aaaaagatga
     3841 gatttgaaat ggttgaagtt aatataaaaa ttaaaggtga agtaagtgaa ataattgact
     3901 caattagagg tatatttaat agctttgatg aagagttaat attagatata aaatcagagt
     3961 aagaatataa aggtagatac gtagaaagtt ataagtaatt caagacttaa attaatttct
     4021 agttttttta atgcaaaaat ttaataaatt tcaaaatata ataattctga catctaaaaa
     4081 gagctattaa aaatgaataa tattgactat taagatatga atagtgtaca tatattaaat
     4141 taagatatca agtaggttaa ttttatataa tgatggggct caaattgaag aggcgatata
     4201 aaaatgtatt aatatatata aatataagaa ctataataca tattaactgt aaaattaaat
     4261 ttatagagtt atatttaaca tttaatgaac tgaaaaataa attagcaaat aatgaaaaaa
     4321 caacagtaat aaatgagaat aacaaagcat tctcaggatt agtggatttt tctaattgag
     4381 ctttaatcct agtttgttca atttcgatat ttctaatatt attagtatta tcaatttatt
     4441 tgtaataatt atcaattttt gcgaaatatt tttgataatt tatagaactt tatatatttt
     4501 ccttaaagta tttttagccc ttattgtttg gtttccaatt ttaaaactca taaactccca
     4561 ataaattcat gtataatata ccttaattta taatatattt aagagtttat ttcagtaatt
     4621 tttcgaacga ttttgatata tttcatttat agttttcaaa aacttataca aaaaagagtg
     4681 tttcattttt ctaaacactc tttttataag aatttgaata ataatttttt aaaaattgat
     4741 aatatggtta tttctttttc aagtttatag tttcctcatt tccattccaa gatataatta
     4801 gcttgtaact agattctcta tttgaataaa gacctggtct ttgtattttt attgcttttt
     4861 cttgacctgc ttctaaaatt tcatttttta caactacaac tgcatcttca ccatctataa
     4921 attgatagga ataattaaat ttttgttcaa ctacatcttt actattttta tattttattt
     4981 ctaattttga gtgtccttca gtagttacaa atttagcttt ccaattttct gattctccaa
     5041 taaagacatt tttatcattt tttatattaa gtatagctat cacagagatt attaaaacta
     5101 taagaatacc tataaaaaat tttttcctca tatcaatccc tcctttgaat aattataaca
     5161 tgagaatagt tattcaatac taattttaaa aatattgtgg atttatattc tattacaata
     5221 gaatgtctgt tataacaaat aaaaagtaag gattaatatg aattataggt taaaaaaatc
     5281 tgtaacaact attttatctt tactcataat gacaatacta ttaccaacaa atgcaagtgc
     5341 tagtaaggta gaagattcat ctatgaaagt aatgtattca gagaaacaag taacagacat
     5401 ggacaaatta tacgatatgg ctaaaaatgg aattactgat gttaaaagtt ccaatgaaaa
     5461 aggtataatc aaaaatgcaa gaacaggaga gtccatatac gttgattcta tatcaacaac
     5521 tcaattatta gaagttaaac aatctaaaaa tttaacatca aagatatatt ctaagacagt
     5581 ttttttgata ctaatacaat aaagcctctt agaaaagcaa gtatatcaga agatggatgg
     5641 gataaaactg gtggtgtaaa agcatctggt acagttacat atacaatatc aaaaggagat
     5701 ggagcatttc aatatatttc cgttagtaaa gtttctggaa actggtctat tgcagacggc
     5761 cagattcgtg taacaaatag aagggtgcat ataaatcaaa ctggatttat ttctaatggg
     5821 ctaggaatta agcagcaaca aaagaaagag ccaacatcaa attctttctc atataatgca
     5881 ccttctagtt ggaaaccagt aggtagagat tcagttcaat acaattgtgg gtttgaatta
     5941 attgcaacat tagtaagaga aggaagttct tgggcattca atttcattgt tcaaccttaa
     6001 aatattttaa gtgaatttta ttaaaatcaa agatatacat atactttaaa attagagaat
     6061 gtaaattaat ttgtgttttt aaagataatt cctacgttat agtaacaatt gcttatttac
     6121 tattaatatt tcttaatata tgaattttat tttaacaaat tataaaaaat gtatattgag
     6181 atgtttttaa atatttttgg aaattatata tattataaca attatagcaa atttttaggg
     6241 tataattttt aaaattatat tctactatca aaagatatga ataaatgatt gtatatatta
     6301 tatcaatttt tatatttcat agtccatttt ttcatcacat tttcgcttgt caagaataat
     6361 atctttaaga gtgaagcgtt tgatgtaaat ctggactttg tttttgagac atttttgaaa
     6421 tatttattaa atccttcgat tatacttgta atatatatta tagatattac ttattttagt
     6481 aaaactaaag gctgatgcta caacattcca gttatttcca aacgttttaa tttctgaagt
     6541 atactgttta cattattttt aatctatttt atctaaagtc gataaggctt tttattcatt
     6601 aactgaccta tatacagatt taaaatcact tattaattct tttgagtcct tgtatgaaac
     6661 atatttaaag tatttcttat ctaatgtatt aaacattttt gtattacaga atgaggataa
     6721 cttgtagcta ttgtctcttt gaaataagtc atctgtattt actcctaaaa ctacttatat
     6781 ctgtagattt tataagttct ccatcttata taatttcttt gattaattgt tttcttaaat
     6841 tattcataaa aaaatctccg ttacgatatt tgattatatt taaatcattg ccataaagaa
     6901 gacttttatt aactttgaaa aacaaattat tttacattac tggtgattag ttgctacttt
     6961 cttttgatat taattctatt agattttata tagttatata tctctttcta actaagtaat
     7021 ataatattac tgttatagta gctactaata ttattgctat tattgtaata tctaatcttc
     7081 ttgtaaataa gcctattgat atatactcaa gacctgttac atagcgataa agtacttcta
     7141 gtttaacagt ttttttctta tctatatttc catatattga ttctaattta ttaaaatatt
     7201 ttttactaca aaaagcagtt ttaggtttaa ttaaatatat tattgtagtt cctattataa
     7261 ctattattcc aataagtata agattgatat ttccaaaatc catagcagat acctcctgtt
     7321 aaatttactt ttcttaatca aagtatatca gtaacacaag tataaagagt atctaaaata
     7381 ttacaaatat taaatgagaa aaagatggta ggttaacccc accatcttat tttttatcta
     7441 ttaataaatg atgctacaaa agttagtata aaaaatgaaa caaacaatat aatgaacttg
     7501 tgtaatttac ttagttgaaa tactttatta caaaagaata aaactagtgc tgagataatt
     7561 gcaaaaagta gatattttat aatcatttta tcacctattg atttcttaca gattgtaagt
     7621 aatatttggt tacataagcc ccacctttat tataacgcat ttttttaaat ttaaagattt
     7681 ttccagatgt tatatttgaa ccaagatgca ttgtaattgc tccaactata gcagctgcaa
     7741 gatactgtcc tataccagga atggcaccag ccaaaagccc tgcaacccct gtaccagcac
     7801 cagctaaagt taatgcaact cctttagaaa gatataaatc aaagcctgtc ttagtccaaa
     7861 ctattttatt tgttccagct tttctataat ataattcatt ttcaataaga gtagtgtcga
     7921 taccattttc cttaaggttt ttaattagct tattaatatc tatgtatgta actgtacctg
     7981 gtttaggtaa ttgagagtca ttgatttcca agtcttctat tgttgttgga ttgttttcta
     8041 tataacttgg gacgttattt gaaatttcca ttgcattggc tgtttgtcca ataggtatac
     8101 ctataagagc tgctaataat gctactgata acaatttctt aatctttgga tttcttagtt
     8161 tcataaaata cctccatttt aaattagtta tatatcaaag atatatatta ttattgtaga
     8221 atataattct taagaattaa ctacattaaa tattaataaa aaatatagta aaacttaaga
     8281 atacatataa taaaacttaa tataaattaa gtatatgaaa taaatatcct gtatttaagc
     8341 ttatagatta taatgaaata taatacatat tataataata aaacgaacta aaattatcac
     8401 actaaagcat aaaacatact ctaatcatgt tagataaatt tttgaatata taatccaaaa
     8461 gtactctcta aagtagagta cttctttttt attaattatt tttatatgga ataaattgga
     8521 atattatatt tgttccaatc tgattgatta catactatta ttagtataaa atttaattat
     8581 acataaattg gtaagaggtg taaactaatg aagattaata aattaccaca tgcagaacta
     8641 aaactaatga agtatatttg gggagcagat gatgtattag cctcaagaga tatcatagaa
     8701 gcaatgaagt tgaaatacga ttggaaaaag agcacaacat tgacattttt aaaaacttta
     8761 gtagataagg gatttttaac tacttattaa ggtagataga tgtacgcatt atactatagc
     8821 tataaaagag aaagattact taaaagttga gacaaagagt ttttttagtt ttatgcataa
     8881 caattcattt aagagtttta tttcagcact acatgatgat gaagttttag atagtaagag
     8941 cttggacaaa ttagaagaat actttaaaaa tttgaaagaa gaagatattg atgactaata
     9001 aagctatcag tatcttttta gtttgagaca attgagagta gcgtttggat ttaatgttag
     9061 ttttatgtga tggtaaaaca ttatataaag aaaagtgatt atttccttag tgtttgatag
     9121 taaaaaatat agagaataat accaattaaa atttaaagat atatgaaata ctacaatagc
     9181 ttaaatcatg taaatatgat aaagatattg aaattataaa aggaatataa ttttagaaga
     9241 aatagttgac agataaatta ccttactata ttattgtatt aataaaataa tacaataata
     9301 tagtaaggag gtaataaatg aaatctagaa aacataatat tacaaaagga ttatttatag
     9361 tttacataat tattttaaca tggataatac tatttaagtt gcaatttgat atctcatctc
     9421 ttgaaaccat gaatcttaga agcataaact tagtaccttt tgctgggtca cttataatta
     9481 ataatagagt agatatatca gagattatat taaatgtggc tatatttgta ccatttggga
     9541 tatatgtgtg tatgctaaaa gaagaatgga gctttataaa aaaagttata cctattttta
     9601 taactagttt agcatttgaa acacttcagt atatatttgc actaggtgca agtgatataa
     9661 ccgatttgat aggaaacaca ctaggaggaa tcataggaat agctgttttt atgctattat
     9721 cgaaaatatt taagaataat accataaaaa ttatcaatgt attggcattg attgtgacaa
     9781 ttattgttgt attattttta ggattagtta tttttgcaaa cttatagaaa taagtatata
     9841 ggtttagctt aaaatagaac gtaagataca gttacatata aaattattta cacataaaaa
     9901 gcacctatat aggtgctttc ctaaattatt aaagaattat tgtatcttga aactctcgta
     9961 tgttcaattc ttgattttct cttgtttttt ctaataaatc taatgcacaa tttatacaaa
    10021 ctttaatttc tgcattggta tttttctcat aataagttat atatttattt ctatatttat
    10081 ctatttcttt tccacaacga ctacacatta caattacgct catatcttag ttcctccatc
    10141 tctaattatt cttaatatat tcaatagtat cacttttctg aaaataatga ttgttaaaac
    10201 tgtatcaaat ttagatttac tttttattat aatgcatttt aataaaaaaa taaaggttat
    10261 gggtaaaata tttatagaaa aaagtatatt attgaataga ttataaaatt ttattatttt
    10321 tcatcagtct taaatacaca ttaatatcta taaaatgaca aaataaaaaa tactatttaa
    10381 atagagatta aaaaattgat gcgaagtatt tgtttgctat atgaataaca atttatttag
    10441 aagttttgat ttgacactgt ataataataa agtaattgct aaaaaagttt gatgatttgg
    10501 atgtattttt agaaattaga aaaatatata cttactgact aatgataatc ttatatatat
    10561 tttagtttga gtgatttaag aaaagtgttt tgaaagggga ttattttata gaataaatat
    10621 atatatatag tagaaaatta catataaaga gtagtattta tttcattacc ttctttatct
    10681 gttccgatgg ggtcttgtag agatacttga gttttatttt ttttgttaga tctaatattc
    10741 attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt tcccttatct
    10801 atgttataag tttctatagc tttaattaat cctattgtac ctatggatat taaatcatcc
    10861 tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct catgtttctt
    10921 tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt taaatactca
    10981 atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa taggtcaccc
    11041 ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga ctgttatcta
    11101 ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt ttgaatggaa
    11161 tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat gaaacaaggc
    11221 gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa atataaaagg
    11281 aaatagtatt tatatatgtt ttggtgttga ggtaaaagat taatgcaaag cattagagtc
    11341 tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta aatgtaatta
    11401 attgtttgta taaagttgat tacaagcaaa aagtatcagt catagttaat tctgttaagg
    11461 atgaaggtgg tgtgttaatt tatatatgta tgggaagaaa ttttgattac aatgaaaaaa
    11521 cttacaaaag atatttatga aaaaacttat agttaaatgt ttatatataa aatattattt
    11581 aggggttgtt tttattattg aaaataagaa agagtggttt tatatcaatc gaatgtataa
    11641 taagtattgc tatattatat gtggctgttt atttagtttc tacatcattg tataattgtt
    11701 atagttttat cagtagaaat atatctgaca gagaaatgtt aagtacagca aaaaaatata
    11761 tagaagatga gaagtataga atacaaaata gtaagtatga gttaattgaa gataagatag
    11821 aaaaaaatta cataaatgga tatgaaatta acagtagaat agagcaaatt ttagattatt
    11881 atcaatgcta tgaaataaat atagagataa aaaatgaatt taaaaaactg aggtttaata
    11941 gctatgttac tagaaaataa aggctctata ttaataataa cattgataat attttctatt
    12001 ataagtactg tgtgcattat gtgtataggt ttaatttata cacataataa tatgtttgag
    12061 ttaggataca aggacattca actaaaagaa atttctttaa gtggaataga aatatcagta
    12121 agtaatgtac tttcttgtgt tgaggaagct attgatgtat ctaataatga agacgaattt
    12181 agagaatatt tcttaggaaa taatatgatt gaatgtataa aaaaaataaa agatacatct
    12241 tatagtagtt tagaagaact ttctttaaaa gtagaaaata atactattta tgacagagga
    12301 gatttttttg aatttgatat agaaagtaat gttagagaaa aagaattttt aaaagcaata
    12361 aaagtaagag tgaaaatcaa aaatccatgg ataggaattg acattgaaga ttatatggat
    12421 aaatctaata acaataaaat tgatgacaaa acattcaata attataaagc atctgaaaat
    12481 aatgaaattc taaatcaatt taaaaatgaa agtgaaaata taaggaatca agaggttgtt
    12541 gaatttacag aagaagatga agaatatgaa gaagaactta aacaaaacat aattgaaaat
    12601 ataaagaaga attttgatga aaaaaaacta gtacaggtat ataagtatag gggtgtataa
    12661 ctttgaaaaa agaaggaatg tgactgtaat agaattaata gtatctatca gtataatact
    12721 cttgatagtt acattatctt ttccaaaaga taatcttgaa aatcataaaa taaatttatt
    12781 tgcacgtcaa ctttgttcag atataaggta tgttaggcga attaatatgt ttagcaacta
    12841 caatacatat atatattata taaatcaagg taaacacgaa ggttatgtat tgagaaaaga
    12901 tggtaaaaac attaaatcaa taatgctacc taaaaatgca aaaatagcta gaagtattga
    12961 aataataaag tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag agatatataa
    13021 taatagatta agaaaaacta taacaataac acctgtaagt ggaagagtac ttctgaaaga
    13081 gggaaaatat gaaacataaa tatggttact tgcttttgga aagtgtagta tcattgtctt
    13141 caatggtgat aattatacta gttttatatt ctatattttt gtctacaata aacttaaagt
    13201 taaaagtaga agataagata gaactacaac aacaaagtct tgaaatcata aaatctatgg
    13261 aaggaattat tagcaattct atgggaataa tgaatgtatc taattatgaa gaaacattta
    13321 aaaagacaac gtctataaaa tgtagatatg tagatgaaaa taataatgaa gaaagcataa
    13381 gtaataaaga aattatattg aatgaaagac gaaataagtt atttgtaaat tctttaaatg
    13441 gcgaaagtag tcaagcaggt ggttatgaaa ttggagatta tgtagatgag atgtatgtat
    13501 taattactaa taatggtcaa tatgttaata ttaaattgaa attatcaaaa cgtagtcaaa
    13561 aatatgagac tgatttcaaa ataaaagtat ggaattttag tgaaagtata taaaaataca
    13621 taaatttaag ataaagaaat gacatcaata agtcatataa aatataatta ttgagaacta
    13681 tataactaaa taatatcaat atgtaaagtg tatagattaa gaatatagag gataataata
    13741 aaaatataga ataagtaatt tgccaaatac ttgtttatat tgtataactt atgatattat
    13801 gatattagta aaaatttgta atcctgactt gacagaattc aagtaagaga tataatttta
    13861 aaggcatagt aatgtaaaat ttactaaaaa ataagaaatt tattttaata tggaggaaca
    13921 aaaaatggta tcagcaggtg attttagaaa aggtgttaca tttgaaaaag atggacaacc
    13981 atgtttagta gttgattttc aacacgttaa gccaggtaaa ggagctgctt tcgttagaac
    14041 taaatacaaa aacttaaaaa caggagctat aagagaagaa agtttcaatc caagtgaaaa
    14101 attccctaag gctgttatag atacaagaca aatgcaatat ctatacaatg atggtgagtt
    14161 atattatttt atggatcaag aaaattttga acaaatacca ttaaactatg agcaagttga
    14221 agatgcaatt aagttcttaa aagaaaatga agttgctaca ataagatttt accaaggaca
    14281 accattccaa gtggaagcgc caaactttgc agagctagaa gttacagata cagagccagg
    14341 tataaaaggt gatacagcaa gtaatgtaac taaagcagct acagttgaaa ctggagcagt
    14401 tgttcaagtt ccattattta taaatactgg agataaagta aagatagata ctagaacagg
    14461 tgaatattta tcaagagtat aaaaatttat gtatataaaa gcttatatgt cttaataata
    14521 taagatatat gagcttttag tttatatata aaaaagaaag cgaggggtgt tacatgaaac
    14581 atttatatga agaagttgca tacttaaaag gcctagcaga aggactagaa ataagtgctg
    14641 aaagtaaaga agggaaaatg atacataaaa tagttgatgc attagaagta tttgcagatg
    14701 ctattgtaac attggatgaa gaacaagaag aacttcaaga ttttgttgaa tctatagatg
    14761 aagatttagc agatttagaa gaagatattg ctgacatgga agaagatctt tatgaagaag
    14821 atgacgatga ggatgatgag gacttcagct atatagaaat ggaatgccca aattgtggag
    14881 agttagtaga aatagacgaa gatttgttat atgatgatga agtagatgtt gtttgtcctg
    14941 attgtaaggc tgtgatatta tcatcagaag atgattgtga tgatgattgt acatgtggtg
    15001 gttgtccaag ttgtgatgat agagagtaaa aattcataag aattaaaact gcctatgatg
    15061 cctatgagat attttgcttc ataggtagtt ttatttttat gaggaagggt attatgtatt
    15121 ttggttttgc aatacttgga caaaaatagt acactagaat ttatagaata ggattttgga
    15181 gggatgtaaa tatgaaaata agtaaaaaat tactagataa tatacaaaga tttgaaaatg
    15241 ttataaatta taaatttaaa aataaagaat atatactaga agctcttact catagttcat
    15301 attctaatga aaataaaaaa tataatttta acgaaaggtt ggaattttta ggggattcgg
    15361 ttttaggcat agtgataagt gattatttat ttaatgaaga agctaatctg cccgaagggg
    15421 aactaactaa acttagagca aatatagttt gtgaagattc gttaagtgaa gtagctaatg
    15481 atataaattt aggtattcat atgttattag gtagaggaga agaagcaaca ggaggaagac
    15541 ataggacttc aatattagca gacgcatttg aagctgtaat agctgctata tatcttgatg
    15601 gaggttttga aagtgcaaga cagtttatac ttcatcatat ggaaaatata atttatgatt
    15661 caagaaaagg aaatatattt agagactaca aaacgcatct tcaagaagtg ttacaaggga
    15721 atggagaaaa taatatctgg tacaggctta tagaagaaaa aggaccagac cataataaaa
    15781 gatttgtaat ggaagttgga attaatgacg atgtattggg tataggagaa ggaaagagta
    15841 aaaaagaagc tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta tgggaaaaat
    15901 aaatataata tgtaaaaata taaaaagaaa tatttatatt agaaggatgt tgtagatgaa
    15961 aaagagaatt atacctatat ttgttcctca caaaggatgt cctcatgatt gcatattttg
    16021 taatcaaaaa aaaataacag gtgtgagtac tgatgttact agtgaagatg caagaaatat
    16081 aattgaggaa tgtttagaga ctatagataa agatgcagat gtagaaatag cattttttgg
    16141 tggtagcttt actgctatag atattgatat acaaaaaagt ttattatcag ttgctaaaga
    16201 atatgtagaa aaaggtcttg taaaagatat tagaatgtct acaagaccag attgtattga
    16261 tgaagatata ttaagtatgc taaaagaata taagacaagt ataattgaac taggagtaca
    16321 atccttagat gaaaaagtat tattagatag tataagaggt catcaatctg aaattgtata
    16381 caagagttca aagatgataa aaaatagtgg aatcaaactt ggattacaga tgatgattgg
    16441 tcttccagct gacacagaag aaaagtgtat atttacagca aaaaaatttg tagagctaaa
    16501 accagattgt gttagagtct atccaacttt ggtagtaaaa gatacaggtc ttgaaaagtt
    16561 aatggaacaa aataaatata atccatttac tcttgaagag agtgtaaata tagtaaaaaa
    16621 agttcttgta ctgttttatg ttaataatat caatgtaata agagttgggc ttcaagctac
    16681 tgatgatata caaattggaa aagctgtttt agcaggacca tatcatcctg catttagaga
    16741 gttggtagaa gcagatatga taaaagatta tttagaattt gttattttgc aaaataaaaa
    16801 tataaagcaa atgttggtca aatctaataa aaagaatatt tctaaaatca taggaaataa
    16861 aaaaacaaat gttaaatata tggaagaaaa atttggtgtg cttttaaaaa cacaagaatc
    16921 cgatttagat ataaatcaat tagaaattgt gttagatggg aaaagtttaa ttagtgctaa
    16981 tatgagagac attcatagaa aattgtatga catttatgac ctttagtttt aataatgaag
    17041 ggagagaaaa gatttgtatt taaaaagact agagttaaag ggatttaaat cctttccagt
    17101 aaaaacagat attattttta aagaaggaat aacagccata gtaggaccaa atgggagtgg
    17161 aaaaagtaat atatctgatg ctgtaagatg ggtattagga gaacagagta ttaagagtct
    17221 tagaggtgat aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa aacctatgaa
    17281 ttattgtgaa gtggctctta ctattgataa ttctgaaaat caattggaat tagattttac
    17341 agaagtaact ataagaagaa gggcttatag aaatggtgaa agtgaatttt ttctaaataa
    17401 taaaagttgt agattaaaag atataaaaga agtcttttta gatactggta ttggaaaaga
    17461 tggttattca atcatagaac aaggaaaagt agatgaaata ttaagtaata atcctttaag
    17521 cagaagaaaa gtttttgatg aagcttgtgg catatcaaaa tatagatata aaaaacagga
    17581 agcagaaagg aatttaagta atacaaagga aaatttagaa agaatagatg atgtatatat
    17641 agaaattgaa aatcaattaa agcctctttt taatcaacag acaaaggcaa aaaagtactt
    17701 ggaaataagt gaaaaattaa agatacttga agtaaatagc tttataagag aaattgaagg
    17761 aatagaaaaa gaattaagtg aggttaatga acatcgtaag gttattgaaa aggagctcaa
    17821 tgaaaaagaa gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta ataaagaggt
    17881 agaggtatta caagatgtaa ttgaaaaatc tgtagattat ataaattcaa ttaaaggagt
    17941 tatatcaaaa aaagagtccc aaataaattt aattaaggaa agaataagaa attttacgaa
    18001 tgaaattagt agaaaaaatt tagaaataaa tgatataaaa gagaagctaa atgaaaataa
    18061 gcaatacata aaagaattag aaagtaataa attatcaggt agtgaagaac tatctacctt
    18121 gcaggaaaat ataaaagtat tggaggcaag taaagataaa caaaaaatta agttagaatc
    18181 cctaaataat gagatagaat tattgaaaga aagtataatt gatatattaa ataagaaaca
    18241 agagtttagt aataagctgt ccactttaaa cgcaaataaa gaaaatatga atatcagaga
    18301 tgaaaatata aattcagaga taacagagtt aaacaaaaat attgaaataa aatcatctga
    18361 attagatact ataaataaag aatttaatat gcaaaatgaa aatttaaaga atgtaaataa
    18421 tagacataaa gaattatcaa ttaatttgca ggattcaatt agtgaacaca ataaactaga
    18481 agatgaaata cagaaaagta aatataattt aaatggatat aattcaaagt taaatgtcta
    18541 tatagatatg gaaaatcatt atgaaggatt taatagagga gtaaaagaag tactaaaaaa
    18601 taaaaattta aaaggagttc atggagcatt aggacaaatt ataaatgtac ctgaaaaata
    18661 tgagaaatct atagaagcag ctcttggtgc ttatatgcaa aatataataa ctgataatga
    18721 gtttagtgct aagtctgcaa taaactatct aaaacagaat aatttaggta gagtaacatt
    18781 tcttccatta aatataatta aatcaaataa agtaagtcta ggaaatttaa aagcaaatac
    18841 taagttcata ggaatagcaa gtgatttaat tacatttgat gaaaagtata gaaatataat
    18901 tgaaaatata cttggaagaa caatacttat aaataatata gatgagggca taaaatttgc
    18961 aaaagaaact ggtcataggt ttaaaatagt aactttagat ggtgaaatct taaatccagg
    19021 aggttctttg actgggggaa gtctaaaaac taatgggaat atactatcga gaaaaagata
    19081 cattaatgaa tatacagaaa agataagtaa tatcaagaat gaaatttcac atctagaatt
    19141 aaaaagagaa tctttagaca aagatgtgaa aaatatcaaa aatgaaattg attcacatga
    19201 aagtaaaata aaagatttag aaaaaagtat aataataaaa agtacaagta taaagaatgt
    19261 tgaatctgaa attgaatcgt taaaaggtag tattactaaa ttagaaaatg agaagaatga
    19321 tttaaattct aatctaaatt atacacttga aaaaagcgat gatgttagaa aagatatgga
    19381 agagttagat gatttataca ataaaaataa agaaaaaatt gatgcattga atgaggaaat
    19441 aaaaagatac aatgatttat atgataaaga aaaatctgaa tttgatgaat tgaacttaag
    19501 tttagtgaag aaaacagaag cttataatag tatagttaga gatataaaaa gaatatctgg
    19561 tgaaaattgt gagctagaag aaaagaataa acaattagaa gagtcattaa attatgaaga
    19621 acatgaaata attaaattac aagattctat agccactgaa gaaaaagaaa aagaaaattt
    19681 aacaaaacaa ctaggggata gtaatagaaa tcttgaaact agaaaaatag caaaagatga
    19741 tttaaaaaat agttttgatg aaattaataa agaattaaaa actatagata gacagcatat
    19801 agagcttaaa gaaagtttat ttaaagttgg tggaagatta gaaagattaa aaactagtca
    19861 agacacatat ataaataagt tgtttgagca atatgatatg actttagtac aagcattgga
    19921 aattaaagat gaagatttgg atatagatag aaaattctta gaaagtctaa agagagaaat
    19981 aagaagtctt ggaaatataa atataggctc tataaaggaa tatgaggaaa taaaagaaag
    20041 atacgatttt tatagcgaac aaaagcaaga tttagaagag tctatggaag agatagaaaa
    20101 acttatacac actttagaag aaaatatgaa atctgaattt gaaattaagt ttgaagaaat
    20161 aagtaaaaat tttaaatatg tatataagag attatttggt ggaggttgtg gagaattaac
    20221 tattttagat aaagaaaatt tattagaaag tgatatacta ataacagctc aacctccagg
    20281 gaaaaaaatg aaaaatttaa atctattatc tggtggagag aaagctttaa cagcaataag
    20341 tatattattt gctatattga ttacaaagcc aacaccattt tgtatattag atgagattga
    20401 agcgccactt gatgatgcaa atatatttag atttggagaa tttttgaagg atttatctaa
    20461 agagactcaa tttatatcag taactcatag gagaggaact atggaagctg ctgattatat
    20521 ctatggagtt acaatgcaag aaaaagccat ttcaaaagtt attagcttaa aattaaaaga
    20581 agctcaggaa ataacagata ttatataact tagattgaca taggaggaat aacagtgtta
    20641 aaaaaattat ttggttttgg aaaagacaag gaaaaagaaa tagaaaaaaa agatgctgaa
    20701 gaagaaatag aagttgaaga cagtgttgat agtttagaaa acttggaaga aaccattttt
    20761 tcaggacttg aagaagaagt gatagataaa gtggaagata ttgaagaaaa aactgaggaa
    20821 agtgtcaata atgaaggcac tgaagaaatc gaaaattttg aaaaaataga agttgagatg
    20881 gatagtaatg caaaagaagt agaaaatatt tcaaataatg attctcaaga agagataatc
    20941 gaagaattag aaaattatga tgaaattgag tctaaagaga tagaagataa agaagataag
    21001 aaagttaact tgtttgaaag actaaaacaa ggattgacaa aagcaaagca aggaataact
    21061 gatagaatag atgaagtttt aaaatcatat acaaaaatag atgaagaatt acttgaagat
    21121 ttagaggaaa ttttaataac agcagatgtt ggtgtaaata caactatgga tataattgaa
    21181 agactaagag ataaaataaa gcaaaaaggt attactgaac caataaaggt aagagaagaa
    21241 cttaaatcta tagttgaaga tattcttaca aatgaaaact ctacactaga tatagaacca
    21301 gcaccatgta taatattaat ggtgggtgta aatggggtcg gtaagactac tacaataggt
    21361 aaactagcaa atagatacaa aaaagatggt aaaaaagttt tattagcagc agcagataca
    21421 tttagagcag cagcaacaga gcaattagaa atttgggcaa atagaaccaa tgtagacata
    21481 ataaaacatc aagagggagc agaccctgga gctgtagtat ttgatgctat aaaagctgca
    21541 aaagctagaa aaacagatgt attaatatgt gatacagcag gaagattaca caataaggct
    21601 aatcttatga atgaacttgg aaaagtattt aaaatagtag atagagaatt tccagaagca
    21661 aaacgtgaag tacttcttgt ggtggatgct acaacaggtc aaaatgcagt tgttcaagca
    21721 aagacattta aagaagttgc agatataaca ggtatagtac ttacaaaact tgatggaact
    21781 gcaaaaggtg gagtagtcct tgcagtaaaa tcagaagtag atgtacctgt aaaacttatt
    21841 ggagttggag aaagtgtaga agacctacaa gatttcaatg caaaatcatt ttcagatgca
    21901 ttatttggta actaatgttc ttttaaaaat aatggttgac aagagtgttg ttatgatata
    21961 aaatacaata gtgtaaagta aataaccttt acagtatact gctaggaagt gtaagatatg
    22021 aatatagaaa aaatggtcga aattggattg ttatttgaac agtataaaga attattaact
    22081 gacaagcaga aagaaatagt tgctctatac tatgaagaag attattcttt aggtgaaatt
    22141 agtgaaaatc tgaatgtatc aagacaaggt gtttatgata ccttaaagcg ttcagaaaaa
    22201 atactaagag attatgaaga aaagttacac ttagtatcaa agattcaaga gcaggaaaaa
    22261 aatattaaga ttataaaaga taaaattatt gacattaaag aagatttatt gcacaataga
    22321 gattgtgcta atttaatccc taagctagaa aatatagaag atgtatgtag ggagatgata
    22381 aaatgatatt tgaaggattg tctgataaac tacaaggggc atttagtaag ttaaaatcta
    22441 aaggaaaact tacagaagta gatgtaaaaa atgctatgag agaagttaaa cttgctcttc
    22501 ttgaggcaga tgttaacttt aaagtagtta aagatttcat aaaaaaagtt caagagagat
    22561 gtgttggaca agaagttatg caaagcttaa ctccagctca gcatgtaatc aaaattgtaa
    22621 atgaagagct tacaagttta atgggagatg tacaaagtaa agtaatgatt tcctcaaagc
    22681 ctcctacaat tttaatgatg gttggattgc aaggtgcagg taagactact acagcaggaa
    22741 aacttggagg atattttaaa aagcaaggta aaaaacctct attagtagct tgtgatattt
    22801 atagaccggc agctataaag caacttcaag ttgttggaga aaaattggat atacctgtat
    22861 tcaatatggg agataaagaa aatccagtaa atattgcgaa agcaggttta agccatgcga
    22921 ttaaaaatgc taatgattta gttattatag atacagctgg tagacttcat atagatgaag
    22981 tacttatggc tgagttaaag tctattaaat cagaagttaa gccacatgag atacttttgg
    23041 ttgtagattc tatgacaggt caagatgctg taaatgttgc agaaagcttc aatgaggcac
    23101 ttggtgttga tggagtagta ttaactaaat tggatggaga tactagaggt ggtgctgcac
    23161 tttcaataag agcagttaca caaaagccta taaaatttat ggggatggga gaaaagctag
    23221 atgatataga acctttccac ccagatagaa tggcatcaag aatattaggt atgggagatg
    23281 tcttaagttt aatagaaaaa gcacaagaaa atatagactt agagaaagct aaagaattag
    23341 agcaaaaaat taaaaaacaa gagcttgatt ttgaagattt cttacaacaa atggaacaaa
    23401 tacaaaatat gggacctctt gacaagatac ttggtatgat acctggtatg ggaaatgtaa
    23461 aagaccaact tggagatgta gatttgaatg gcaaagaaat gaagagaaca aaagccataa
    23521 ttcagtcaat gactacatat gagagaagaa acccaggagt attaaatgct tcaagaaaga
    23581 aaagaatagc taagggcagt ggaactagtg tacaagatgt aaatagactt ataaaacaac
    23641 ttaatgaaat gaaaaaaatg atgaagatgt ttgctggttc acaaaagagt atgaagaaaa
    23701 ggggcggctt aggcggttta ccttttttta aataaaatat aaaaaagtaa aaattattcg
    23761 gaggtgactg taatggcagt taaaataaga ttaaaaagaa tgggagctaa caaaaaacct
    23821 ttctacagaa tagtagtagc tgattcaaga gctccaagag atggaaaatt tatagaagaa
    23881 ataggatact ataatccaat ttctgaacct aaacaagtaa gaataaatga tgaaaaagct
    23941 ataaaatggt tagctactgg tgctcagcca acagaagtag ttaaaaaatt acttgtaaaa
    24001 aacggagtaa tagaaaaatt cgaagcttct aaacaagcaa aataattttt tcttggcaat
    24061 tagtttaagt aagggctcat tgctaaggtt actttaggag atgaaatata tgaaagagct
    24121 agttgtagat atagctaagg ctctagttga taatcctgac tcagttgttg ttgaggaatt
    24181 tgaagataat gatggtatcg tcttaaagct tacagttgct caagaggata tgggtaaggt
    24241 tattgggaag cagggaagaa tagctaaagc tataagaact gtcgtaagat ctgttgcaaa
    24301 tagagaaaat ataaaagttt ctcttgagat agtataaaat aggaggatta ggtataatgc
    24361 ctagtccttt ttatataaat gcgtagttta agattttatg atgatatgac cacataagat
    24421 taaatttttt aagaaggtga taacatgaaa gaaaaattaa ctcattttaa ggtaggtcaa
    24481 atagtaaata cacaaggttt aaaaggtgaa gtaagagttt atccacttac tgatgatata
    24541 gagaggtttg atgaattaaa agatttctat ctgggaaaag atttaaataa taaattggct
    24601 gttgaaaaag taagatacaa aggttctatg gttataatga agataaaagg catagacagc
    24661 atagagaaag cagaaaaact aaaaaataaa tttatgtatg tatctagaga agatagcaga
    24721 gagcttgaag aaggcgaatt ttttatagca gatatgattg gaatggaagt tttaactgta
    24781 gatggaaaat atgtaggaat actagaagat gttcttcaat actcagcaaa tgatgtttat
    24841 gtagtaaaag gtgaagaaga taaagaattt atgataccag ctataaaaaa atttgtacct
    24901 acaatagata ttgatgaaag aaaaatgatt atagacccta ttaagggaat gatagattag
    24961 gtggtaatat gagatttcat ataatgacac tttttccaga aatatttaat tcgtacatgg
    25021 atgaaagtat aatgaagaga gcagttgaaa aaggtataat tgaggtttat atatataata
    25081 taagggattt ttctaataat aaacataaaa aagtagatga ttatccattt ggaggaggag
    25141 tggggatggt aatgactcct caacctatat atgacacata taagcatata ataacaactc
    25201 acaatataag taatccaagt gtaatttatc ttactccaaa aggtaaggta tacaatcaat
    25261 ctatagcaaa gcagatgtct ttaaaagaag atataatact attatgtggc cattatgaag
    25321 gagtagatga gagaatcatt gatttaatag ttactgatga aatttcaatt ggagattatg
    25381 tattaactgg gggagagctc ccagcactta taatgataga ttctatatca agattaatac
    25441 caggtgtatt aaaccaagaa gaatcatttg aagaagaatc atttaaagat aacttattgg
    25501 aatatcctca ttacactaga cctagagatt ttgaaggatt aaaagtacca gaagtgcttt
    25561 tatcagggaa tcataaaaag atagatgagt ggagaagaga agaatctata aggataacca
    25621 aagaaagacg atttgattta tataaaaagt ctaatgaaaa ataaaaatat aatgaaaaat
    25681 taatatatat gtatacaata aagtacctat aaatggaatt aaaaacataa aagtgcttgt
    25741 aataactatt gaagtatagt ataatataaa atgttgaaaa tacgggcgtt cctctgttta
    25801 cactggtcgg gtagttaaga acgtctatga tatctaggag gaagaaagat gaatgaaatg
    25861 ctaagagcaa ttgagcaaga acaattaaaa aatgaagttc ctaattttgg acctggggac
    25921 acagttaaag tacacgttag aattatagaa ggaaaaagag aaagaataca agtattcgaa
    25981 ggtgtagtat taaagagaca aggtggagga gcaagagaaa ctttcacagt tagaaaaatg
    26041 tctttcaatg ttggtgtaga aagaactttc ccagttcact ctccaaaaat agaaaaaata
    26101 gaagttacta gaaaaggtaa agtaagaaga gctaaactaa actacttaag aggtagagta
    26161 ggtaaagctg ctaagataaa agaagctaga aataaataat taaaaacttt acttataaat
    26221 ggggggctga tttatcagtc ctctttctat tttaatgaaa attacagcaa ataattatac
    26281 tagaaataaa tagaaatatt tgttgaaaat atacattatt tatctatgaa tttatagctt
    26341 ttaaaatgaa aaataaaatt ggtaaatatt ggattatatt ttgtaacgat attaaaaata
    26401 agagaacagt attatgtaaa gtatgacgtt aaaaagatga caatattata gtttgtgaag
    26461 atagattttt taagtggaat tagaatataa taatataata ttaataagga gtgatacatg
    26521 tgtcaaatga atacgaagag tatcttatgg atgacaatct acatataaac tggtatccag
    26581 gtcatatgaa aaaaactaag gagttagtaa aaaataactt aaaattgatt gatgtagtaa
    26641 ttgaactttt ggatgctaga atcccgttta gtagtaaaaa tccagacata gacaggcttg
    26701 taggagataa gccaagagtt gtcgtactta ataagagtga tatggcagat agggataaat
    26761 tgaatcaatg gattgaatac tataagaaaa ttaatatcaa agctatacca gtagatacaa
    26821 taaaaggtgt tggtataaat aatataatcg aagaatgtaa aaatgtaact agagaaaaaa
    26881 tgagttcttt aaaagacaaa gggagaaaag aaagagctat acgaataatg atagttggag
    26941 ttccaaacgt tggtaaatcg tctcttataa acaaattaac aggtagaaaa agcactcaaa
    27001 ctggagataa gccaggagta acgaaaggga aacaatgggt tagattaaag ggaaatcttg
    27061 aacttttaga tacacctgga atactttggc ctaagtttga agaccaagaa gttgcattaa
    27121 atttagcatt tagtagagct ataaaggatg agattttaga tactgagact ttggctttaa
    27181 gacttataga aaaacttatg aaaatagaac ctgagaagtt gaaagccaga tataagttag
    27241 attgtctagg agaaactcca attgaaacta tggatatgat tggccataaa agagggttta
    27301 taacaggtaa aaaagaacta gattatacac gcatagccgc aactgtatta aatgaattta
    27361 gagatggaaa aataggaaat ataacattgg aagttccaga aaacgttaaa agatagattt
    27421 taaaacaaaa tcaaagattt aatgttaaaa aaataaaaaa gccttttagc aaaggctttt
    27481 ttattttttt gaaatataga caaatatctc catataaaag tataaattag gttagaaaac
    27541 ctttaaaaat attaatttaa gacaaataca cctaatattt tgaaaattaa aatgagtgtg
    27601 attattttaa attttagcat attgacttgc ttgtttcgta taatatataa taagcacaaa
    27661 taaatacaat atattaaaaa tttaatttat ttaattttat taatttaata ataagttctt
    27721 attgtttaat ttaatttatt tatgcaagcg taaacaatcg agacatttta ttaatttaat
    27781 aaggttaaaa agggggattt tataatgggt aagaccaagg atgttatagt atttggattt
    27841 gcgttatttg caatgttctt tggagcaggt aacttaatat ttccacctta cttgggaata
    27901 attacaggtc cagaatggtt aattgccttc ttaggattta cgtttgctga tgctggattg
    27961 gcacttctag cagtaatggc tacagctaaa ttcgatggaa acgtagttga aatgtttaaa
    28021 agatgtggta tgaagttagg gattcttata ggttgtgcag acattttatg tataggtcca
    28081 ttcttagcta taccaagaac aggtgcaaca acttatgaaa tgggtataat gcctttattt
    28141 ggaacttcaa ttccagtgtt attattctgt atactttttt ttgcgctttc atatgtacta
    28201 acaataagac cttctaaggt cgtagatata gttggacagt ttttaactcc agctctttta
    28261 atagctctag catttataat tataaaaggt attatatcac ctcttggaga tatagtagat
    28321 aaacctatga tacctaatgt atttgcagaa ggtataggtc aaggatatca gacaatggat
    28381 gcttttgcag caatagcact tgcatcagtg ttaatagtat ctttaaatga taaaggatat
    28441 tctactatta gtgacaagtt aaaaatgatt ggtaaggcgg gggttcttgc ttgtggagga
    28501 ttagctctag tttatggagg attatgtttc cttggagcaa ctgtatcaac aatgtatgga
    28561 acagatgcag tacaatcaca agtaatagta aatataactg aaggattact tggaaatgtt
    28621 gggaaagcga ttttagcagt agtagtatca ttagcatgtt taactacatc tataggtctt
    28681 acatctgcca caggtcaata tttctctaga cttacaaaag gtaaattgag ttatgaaaaa
    28741 atagtattag ctgtatctgt attcagtgca gtagtagcat cttttggtgt tggaactata
    28801 ataaagatag catctcctat actaagtata gtttatccac catcaattgt tttaataatt
    28861 ttagctttct tcaatgaaaa aattaagaat gataatgtgt acaaaggagc agtatatatg
    28921 tcattgcttg taagtatact aacagttata tctagttatg gagtagatgt tccagtagta
    28981 aaatctttac ctcttcattc attaggattt aactgggtgg taccagttat aatagctgga
    29041 ataattggta attttatacc atcaaagagt caatctaata cacttggaac aaactaagtt
    29101 ttgtttaatt aaaatataat caattgtaaa taaatgagtt aactaaaagt aaaaggagtt
    29161 atttgtacaa ataactcctt ttattttggg att
//
LOCUS       DAMKPG010000045        27329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMKPG010000045 DAMKPG010000000
VERSION     DAMKPG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.048"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            142..2160
                     /locus_tag="PC621_002070"
     CDS             142..2160
                     /locus_tag="PC621_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4109226.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            2673..4685
                     /locus_tag="PC621_002071"
     CDS             2673..4685
                     /locus_tag="PC621_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4109227.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            4913..6826
                     /locus_tag="PC621_002072"
     CDS             4913..6826
                     /locus_tag="PC621_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109228.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTDNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            6819..9038
                     /locus_tag="PC621_002073"
     CDS             6819..9038
                     /locus_tag="PC621_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF4109229.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            9147..9977
                     /locus_tag="PC621_002074"
     CDS             9147..9977
                     /locus_tag="PC621_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF4109230.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            9967..13434
                     /gene="addB"
                     /locus_tag="PC621_002075"
     CDS             9967..13434
                     /gene="addB"
                     /locus_tag="PC621_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF4109231.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            13434..17261
                     /gene="addA"
                     /locus_tag="PC621_002076"
     CDS             13434..17261
                     /gene="addA"
                     /locus_tag="PC621_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF4109232.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            17307..18518
                     /locus_tag="PC621_002077"
     CDS             17307..18518
                     /locus_tag="PC621_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF4109233.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            18505..22032
                     /locus_tag="PC621_002078"
     CDS             18505..22032
                     /locus_tag="PC621_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4109234.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            22163..23416
                     /locus_tag="PC621_002079"
     CDS             22163..23416
                     /locus_tag="PC621_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF4109235.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            23565..24617
                     /gene="ytvI"
                     /locus_tag="PC621_002080"
     CDS             23565..24617
                     /gene="ytvI"
                     /locus_tag="PC621_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF4109236.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            24708..25934
                     /gene="pepT"
                     /locus_tag="PC621_002081"
     CDS             24708..25934
                     /gene="pepT"
                     /locus_tag="PC621_002081"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF4109237.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            26141..27193
                     /locus_tag="PC621_002082"
     CDS             26141..27193
                     /locus_tag="PC621_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF4109238.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        11640 a         2664 c         4833 g         8192 t
ORIGIN      
        1 attttttgtt tttataattt ttttagaagt ttatttttta tgtaattatt tataattttg
       61 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      121 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      181 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      241 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag caaatcaaaa
      301 gtcagtgtca agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      361 tatgatatgc cattggatac aactctaaat tataatcaat caactgagtc atatgaagga
      421 actatcaatt ataataaaga cccagaatat ttaaatgttt gggaactaca agggataaca
      481 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      541 aatttaaaag actataatgt aacgcaggaa tgtataattg aagatataac ttctagaaaa
      601 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      661 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      721 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      781 tataatgctc caatactttt ggttgaaaaa aataatgtac ctaatagtgt aaaatcagaa
      841 ttaaagcgtt taaatcctag agatgtaatt ataattggag atgagaatgc tatttctaaa
      901 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
      961 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1021 aaagtataca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1081 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1141 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg acctcaaatg
     1201 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1261 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1321 gatgatgagt tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1381 gcaggtccat tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacgtatgta
     1441 tctgcatacc ataaagataa tttagaagct aaatcagcta ataaagtata taaaatagga
     1501 ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa acacaataca
     1561 actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc aggacatggt
     1621 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa agactataca
     1681 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1741 acaagagaca cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1801 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1861 gaagtatttt ataagcttaa agataaagat ggagggacta ctaaaactgt agctacaaat
     1921 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     1981 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2041 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2101 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2161 tctacaatga gagcatagaa aaatttaaaa taaattaatc tgcaatttta aatttaaact
     2221 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2281 ataagtgcga aatgaggtat atacagtctt tttatgatta attgtaaaag gatttatatt
     2341 atatgaagaa tttacatatc ttaaggctac agttaattgt caagagttgg agtattcatt
     2401 tgtaatacac aataaaagct taattactat attttattgt gtgtttgtgt gtttgtgtgt
     2461 tttttgtgga tttttatgtc aataaatgat atagtataga taattaaatg tattaaatat
     2521 atttaatttt tttatttttt gagttttata agtttatttt tatatattac ttatatatta
     2581 tttatatttt gggttagata cactttgaca tattttcaaa agttaaaaga ttaaaaataa
     2641 agtagaaatt tatgttaagt aaggagatta atatgagaag aaatacaaaa ttattaacaa
     2701 cagggattct ttcaatggca atcgtcgcac ctacaatggc atttgctact gaatctaatg
     2761 ctatggaaaa taacgctgat ttaaatataa acttagagaa aaaaagtatc gttttaggta
     2821 gcaaatcaaa agttagtgtc aaatttaaag aaaaaccaga tgcagatagc attacattaa
     2881 agtataaatg ctatgacatg ccattggata caactctaaa ttacaatcaa tcaactgggg
     2941 catatgaagg aactatcaat tataaccaag acccagaata tctaaatgtt tgggaactac
     3001 aagggataac aataaacagc aaaaataatc ctaaaacttt aaacggacaa gacctagaaa
     3061 agatgggatt aaatttaaaa gactataatg taacacagga atgtataatt gaagacataa
     3121 cttctagaaa agatgtaaat aaatatttga gaaaaacttc tgcacctatt acagaactta
     3181 caggaagtga tagatatgaa acagcagtta aaataagtaa agagggctgg aaaaatggtt
     3241 cagataaagt agttataata aatggagatg taagtataga tggcattata tcaactccgc
     3301 tggcaaccac atataatgct ccaatacttt tggttgaaaa aaacaatgtg cctaatagtg
     3361 taaaatcaga attaaagcgc ctaaacccta aagatataat tataattgga gatgagaatg
     3421 ctatttctaa aactactgct aatcaaatta aatcgactgt aaatgctagt caaacacgtt
     3481 taaatggttc taatagatat gagacatctt tattgatagc aaaggaaata gataaaaatc
     3541 atgatgtgga aaaagtatac ataacaaatg ctaatggcgg agaagtggat gcacttacta
     3601 tagcagcaaa agcaggtcaa gacaagcaac caattatatt aactgataaa gatagtatta
     3661 cagacaatac atataaatgg ttaaagagtg aggatttaca aaatgcttat tttataggtg
     3721 gtcctcaaat gatatcaaca aatgttataa ataaggtaaa tggaataact aaggatagtg
     3781 ttactaataa tagagtatac ggagcagata gacacgaaac aaatgcaaat gtaataaaaa
     3841 aattctatac agaggatgag atagaggctg ttttagtagc taaatcagat gtacttgttg
     3901 atgctttagc agcaggtcca ttggctgcga acttaaaatc tccaatactt ataacaccaa
     3961 agacgtatgt atctgcatac cataaagata atttagaagc taaatcagct aataaagtat
     4021 acaaaatagg tggaggattg acttctaagg taatgagctc tatagcatca tcattatcta
     4081 aacacaatac gactccaaca gaaccaggaa atagtggggg caagacagtt atgattgacc
     4141 cagggcatgg tggttcagca cctggaaatt catctggagg aatgattgaa aaagattaca
     4201 atttaaatac ttcacttgca acaactgaat atttacgttc aaagggattc aatgtaataa
     4261 tgacaagaga cacagataag actttatctc ttggaaatag aactgctcta tctaattcat
     4321 tgaaaccaga tttatttaca agtatacatt ataatggctc aactaataaa caaggtcatg
     4381 gtgtagaagt attttataag cttaaagata aaaatggggg gactactaaa actgtagcta
     4441 ccaatatatt aaatagaatt ttagagaaat ttaaacttac aaatagaggt ataaaaacaa
     4501 gagtacttcc tagtgattct acaaaagatt atttatacgt tttaagaagt aatgatatgc
     4561 cagctgtact tgtagaatgt gcatttttgg ataatgaaaa tgatatgagt ttaataaact
     4621 catctgcaaa agtaaaagaa atgggtacac aaataggtaa aggaatagaa gattcattaa
     4681 aataaatgga aataaaaata tttaaaaact taatgtaaat ttatactaaa gtcaaaaaat
     4741 gaaatataag tgtatcaata gtaagactgt atgtacattt gcacatgtga atgcaagtat
     4801 atatacagtc tttttttgta gttaaatgca ataatttgat tgtgttttgt aaaatacaca
     4861 atatgatata attagataat atacacttaa tttaattgag gaaggtgatt atatggaata
     4921 tttatttgaa aaaagtagtt taaaagaata tggaaatgaa gctttagata aatatagaga
     4981 attattagat aaaactgata ataattcaag gtgtattctt ttattagtac caaacaataa
     5041 cacaaagata cgatatgaaa gggctctaag attaaattat agtgaagaac tgaaaataac
     5101 aacatatata agttttgtga agaaagaatt agttaaatac tggccactaa taatagaaaa
     5161 atgtgatgaa ataaatacaa aggttgtatc tccaacattt atatcaaata gtttaagtga
     5221 atatttaata gtgcaaaaag ttaaggaaaa aaggatacaa gaaggatatt ttgaagatat
     5281 tacaggaacc aataaatcca tagcaaatag tataatgact aacataaata aatctgcatt
     5341 taatttaatt gattttgatt ttattggaga aaaaatatat tcatcaaaga aaaataaaga
     5401 tagtatatat agattttcat acactcagat ggatgaaatt atatcatatt atataaattc
     5461 gttactatca aattcaattt tagataatgc attaagcata tatttataca atcaatacct
     5521 acttaatgat gatttttatt taaaaaactt atttacagaa gtgagatata taattgtgga
     5581 tagtcttgaa agctcaagta atgcagaagt tgattttata acagaagcat tggataatac
     5641 attacaaagt tatatatatt ttgattatag tagggattat tctgtattta ataatgtgga
     5701 tatagactat ataaatgaga aaataatatc aaaaattaag cctaaagaag aaaatattaa
     5761 aaagaaggag attagaatag aagatttata cttattacct gcaaatatag aattaaatca
     5821 atcaagtcaa ttatacaatg aaatgttaga tttaataagt gaaaaagtta tatctcttat
     5881 agaagaaaat gtatctccaa gagatatagc aattatatct cctataaaca atagtatatt
     5941 ggagcatcaa attagagatt ctttgataga aaaaaatata gatgttttta gtactaagaa
     6001 ggataaaaaa gctgtagatt atccatatgg gaatgcattg gtagtggcta catgtatatt
     6061 ttatggatat ttagatttta taaaagatga tgaatactta agctttttag aaactctttt
     6121 agaaataaat aggataaaag catttaagat ttttaatgaa acaagacatt taagtattga
     6181 taatatagat gaacatagtc agtatcgtga tattttacag tatattgaag aaaaaaagaa
     6241 gagtgatata aaaatacatg aatttttaac tcaattttac atagataaaa tgttaaattt
     6301 aaaagaaggt aaaaagaatg ttgggttatg taaaaagata atagctgaaa gtgagtcttt
     6361 tagtgaaagt ttaagtctac ttaggatgaa aaaagaaaag atatttatag aagctttaaa
     6421 aagtacaata aacgattatt actctgttgt cgatatagaa gagttgaaat ccaaagacaa
     6481 aatagtaata actactcctt attcatatat atcatcaaat atagatagaa gtatacaaat
     6541 atgggtagat ataggaagta atgcttggaa tatgaaaata gaaaaagata taagtaattt
     6601 aattgtactt agaaaatctt ttgaagaaaa aaagatttat acaaatgaaa tggaagaata
     6661 ttataaaaaa tattatctat acaatacagt atataatttg ttattaaatg caaagaaaat
     6721 ctatgcattt aaaagtgaat atgcgattaa tggttatatc caagagagta tattatatag
     6781 tatactttta aaaatctcac ataaaggaga taaaaattat gattaatata aattatagag
     6841 aagaccagat accaataata aattacgata atggtactat ggcagtacca gcagtaccag
     6901 gtgctggaaa aacatttata ataacaaatt tagtagctaa actattactt gaacagaaac
     6961 ataagggtgg taaaatatta attcttacat atatgaatag tgctgtaaat aattttaaag
     7021 gtagaataag aaagattctt gaagagaata aaatagatga tactaattcg tatgaagtaa
     7081 tgactataca cagtttggct gtaaaaataa taaaggaaaa gccagaagtt gtaatgttaa
     7141 gtgaggattt taacatagct gacgatttgc aaaaaagtat tatattaaat gaatgtataa
     7201 gtagatttag atttgaaggt ggagaaagag cttttagatg gtttttaaag gagcaaaaag
     7261 atgaacgctg gaaagaaata gctcttgaag cctgggaaag aggttttttt gaatttgcag
     7321 gaaatgcaat aagtgaactt aagtataagg gaatttctcc agataaactt gaagaaatat
     7381 tgtcaagagg acacaaagga atgcttaaaa ttattttacc aatatataaa ttgtatgata
     7441 gaaaattaaa gcaaaatgga ttgctagatt atgatgatat attaatttta gcagaaaaaa
     7501 ctttaagctt agacgaaggg cttagaaaga agtttcaaac tagatacaaa tatatatttg
     7561 aagatgagtg tcaggactca aatgaaattc aaggtaatat aataaaaatt atatctagtg
     7621 aaaataaaaa cctagttaga gttggagata taaatcagag tataacagga acattttcat
     7681 catcagaccc taaatttttt aaagaattta taaatagtgc agatttctgt tataggatgg
     7741 atatgtcaaa tcgaagttca aaagatatac ttgatttagc aaacactctt gtcaagtatg
     7801 ttacaaagga atttagacaa gaagaatgta gacatgctct tgaagatatg caaataaaaa
     7861 ctgtgccaaa tggaatggga tataaagaaa atcctaatcc agaacattat aatataaatg
     7921 taaagtggta tgagagttgg aaaattgaaa tagagcaaac tgctagatac gttaagggta
     7981 taaaaaataa atatccagat aaaagtatag gtatattggt tccatttaat gaacaagtga
     8041 cacaagttgc taaagaactt agagagaata atctaacctt tgaagaatta ggaccaaatt
     8101 ctttgaataa aaggaaggtt ataaacaaca tagcatatat tattgatttt atgttacatt
     8161 gtgatgatgt agaaaagtta atagttgtac ttaataaagt ttttatagat actgataatg
     8221 aagaagaaat aaagtatttt ttagaagaat taaaaaacta ttctaccgaa gaaattattt
     8281 ataattttga atttagtaag ttagataatg atttaataga atttaaagaa gaaagtgaca
     8341 tatatttaag ttttaaaaga ggaatagata ttataaaaca gatattagaa tatccaataa
     8401 taaggttaga cctcttagtt ttgtttattg ggaaaaaact taatttagaa aaagaagata
     8461 aagctgtact ggattatata tctttttata taaagtatct aatatcagaa aatataaata
     8521 tggatttaga agatgtatat aatttattgt ttgatgtaaa gaatagggtg tttaatcata
     8581 taatagatgt ggtatatgag ataaatggat atgaaccaga gcccggtagt ataacactat
     8641 gtaactatca taagtcaaag gggatggaat gggattgtgt gtttttacta ggtcttgttg
     8701 aatataattt ccctgacaat attaatcaaa agtttcagag tgataaatgg tatctaaaag
     8761 aaaaatataa aaaccctatg gcaattatta aatcagaagt tgaagctata ttaaaaggaa
     8821 gtatttcaac agattatgct cataaaacga agattgattc tataaatgaa aagattagac
     8881 ttttatatgt agggattact agagcaaagg aaatgttagt actttcagga agtgcttata
     8941 gagatgaaag tgatataggg aataagagaa aagagcaaaa accatgtata tatttaagca
     9001 gacttaatca acatattatt gaaaaaaggt caaattaata tataataagt tattaaaaga
     9061 ttgcttgaaa tatatctaaa aggggataaa acaatatgta cagtaagtaa ctactaaaaa
     9121 ataaagttaa gagagaagtg atatgagtga acgataaatt aaaatatttt tcttatagtc
     9181 aaaattcaat caacacatat aaatcatgtc cattgaaatt taaatataaa tatattgata
     9241 gaataaattg gaaaaatgat gatgttggta gtagagagta ttatgaaact ttaaaaacag
     9301 gaagagattt tcatttgatt tgtgaaagat attttagcaa tatacctctt ggaatctatt
     9361 ttaatgagga tgataaaaat agtaaaaagt ttttaaagtg gatggaaaat ataaaaaaag
     9421 ttgttcctat tgaaaaagga aaaacgtatt tgccagaata tgaagtaagg atgaccttaa
     9481 atggggatat aatacaggca aagtatgatt tagttgtaat tggaaataac aatatagaaa
     9541 tatgggattg gaaaaccgag tctaaaaaga ttgactataa acacgtagaa aatagaatac
     9601 aaactattgt ttatatgttt ttagcaaaag aagtaatacc taaagtttta aaaatggaca
     9661 taaatgttaa agatataaat atgagatatt atcaacctga atttgatgac ttaccaatta
     9721 ccattttata tgatgaagaa aaacatgaag caaatagaaa taaaattcaa aattatataa
     9781 gtatgataaa aaacactaat tatgaagaac atacatacga agacaatcta tacaatgata
     9841 tagaaaaggt atataataaa gaagaacgta tgtgctatag aaataaaaag cattgcaagt
     9901 attgtgaatt taataagtta tgtaatggca aagaaataga ttatagtatt ttggaggcgg
     9961 aaatttatgg gacttaggtt tgtattaggt agaagtggaa gtggaaaatc cacctatata
    10021 ctagatgaga ttaaaaaaga agctcaaaaa aatgaaacga catcaattat acttttagtt
    10081 ccagaacagt acacatttga ggctgaaaat agagtaagta agctattctt aggaaaagaa
    10141 aaagataagt atctaagagt tagggtactt agctttaaga cactaagtaa tatagttttt
    10201 tctcaagtag gaggattgac agatgtaaat ataaattcta gtggaaaagc aatgatggta
    10261 tatagagcta ttgaagatgt aagtgaagaa ttaaatgtgt tttcaaaatc aaaatcgcag
    10321 tctggttttg taagctcaat tacagatatg atatcagaaa tgaaacaata caatataagc
    10381 cctgaaatgt tagaaaatat atctggagaa ttggataatg aaactttaag tttaaaatta
    10441 aaagatataa gtaaaatata taattctttt gaagggaaat tgcatgaaaa ttatgtagat
    10501 gctcaagata tgttaacttc attggcaagt aaaattgaat taagcagtta tttagatgga
    10561 gcttgtgttt atatagatga atttacaggt tttacaccaa atcagtacaa tgtcataaag
    10621 agtatattaa ataagtctaa aagtgttaat atctctttga ctgtagatga tatcaactat
    10681 attgggtaca gtaaatctga tatgttttct agaacgaaat ttacttattc aaaattgact
    10741 caattgtgca acgaagaagg cataaaaata ttgccacaag taaatttaaa cacaggtgtt
    10801 ataaaaagat ttgaaaaagt taaggaatta cagcacctag aaagatttta taatgcttat
    10861 ccatataaaa tatattcaaa tccaactgaa aatataaaga taaaagagtt taacaattta
    10921 tattcagagg tggaagagat agccagagaa attgtacatt tagttagaga taagaatgta
    10981 agatatagag atataaccat tgctacaaga gatttaaata gatatgattt tttagtacac
    11041 tctatattta atgaatataa tataccaaac tttatagata agaagagaga ggcaaaaagt
    11101 aatccaatag ttattttaat tatttcagct ttagaaatga agaatagacg atatgggtat
    11161 gaaactatgt ttaggtattt aaaaagtgga cttataggaa ttgataatga tgatataaat
    11221 ttactagaaa attatgtgtt ggctaatggg attaaaggta aaaaatggtt tgatgaaaaa
    11281 tgggattata gaataacaca aagtctttca ggtcaagaaa gtgaatttga actggaatta
    11341 aaagaaaaaa taaatgaaat aaaaaataga gtattagagc ctatagtaat tttacaagaa
    11401 aaattaagag gaaaaaatag agtaaaagaa atctgtagat atatatatga atttttatta
    11461 gatataaata tgccagaaac tatagaaaat ttaatagtaa attttaagga taaaggggaa
    11521 cttgatgttg caaatcaata ctcacaagtt tgggacatag tagtggacat attagaccaa
    11581 atggtggaac ttatgggtga tgaaataatt tctttagaaa aatttataaa attaataacc
    11641 ttaggctttg atgaatatga attaggactg gttcctccta gtatagacca ggtacttgta
    11701 agtagtgtgg atagaatgaa aaatccagac acgaaatatt tatacctagt tggaactact
    11761 gatggtgtgt ttccactgat tacaaaggat agtggaatat tgagtgataa tgatagggaa
    11821 agtcttggaa acaaaggaat cgaagttgat attgatagta agactaggag ttttgaagaa
    11881 cagtttcttg tatataaagc tcttacttcg acatctaaga atttaaccat aacatatcca
    11941 atatcagacc atgaaggtaa aacgcttaga ccatcaataa ttatatcaag acttaagaag
    12001 atatttccaa atatagaaaa taaaagttat ttagtggaag aaaataaaag cactgataaa
    12061 gatatactta aaaaaataac ggttaaatcc cctacattta atgaacttat aaacgtaata
    12121 aaaaattatg acagtgatga ttacaataca gaagagatta atagtatttg gttagatata
    12181 tataggtact atttaaaaga tgaaatctat agttctataa caaaaaaagt tataaaaggt
    12241 cttagttata ctaatcaagt tcataagatt gaagagaaaa aaataaggtc attgtataaa
    12301 agtaattctc ttagcatatc taggcttgaa aaatatgctg aatgtccatt tgcttatttt
    12361 atacaatatg gtctaaaagc aaaaaagcgt aaagagtatt catttacacc accagattta
    12421 ggaactttta ttcataatat tttagatagg ttttcaaaag aacttttaca agataattta
    12481 acttggagag atattgatga aaaatatata gaattaaaga taggtataat agttgatgaa
    12541 ataattttaa agataccagg atatatatta aatagttctg agaggtataa ataccttgca
    12601 tatagattaa agaatatgtt gacaactgct ataactataa ttagtcagca aataaaacaa
    12661 ggttcatttg aaccaataga ttatgaagtt aaatttggcg acaatggaaa atatccacct
    12721 ataaaaatgg tgttagaaaa tggacaagaa gtaagtttaa ttggacaaat agatagagtt
    12781 gatgaatttg aagaaggaga aaataaatat ataagaataa tagattataa gtcaggcaat
    12841 aagtccataa gtctaacaga aatatattat ggtctacaat tacaattact tgtgtattta
    12901 gatgcaatcc ttgaaagtgc aaaggatgaa gatataaata taaatccagc tgcaatacta
    12961 tattgtagaa taaataatcc aatagcaaaa tttaatgaag ataaagatga tgaagaaata
    13021 caagaagcaa tattaaaaga acttagaatg aaggggcttg tagtaaaaga ctctcacata
    13081 gttaaagaga tggataaatc attaatagat ggagaaagga aaaactcttt agtaatacca
    13141 gtaggtctta ctaaggatgg aaatgtaggt aaaagtactt cagcaataag ctatgaggat
    13201 ttcaaactgc ttagaaaata tgtaaggcat gctataaagg atttatgtga agaaatgtta
    13261 agtggagaaa tacgtatagc tccatataaa cataaagatg gaacatcatg tgacttttgt
    13321 gattactcta ctatatgtca gtttgattca actataaaag ataacaagta taaaaattta
    13381 aataataaga gtaatgaaga gataataaaa atgatgaaag gagatgttaa ttagtgagtt
    13441 ctcctaaatg gacaaaagag caattagaag ttataggaag tagagaatgc aatttattgg
    13501 ttgctgctgc tgctggttca ggaaaaacag ccgttctagt tgagcgtata attcaaatga
    13561 taacaagtag agaaaatcca attgatatag ataagttatt agttgttaca tttactaatg
    13621 ctgctgcgtc agaaatgaga gagaggatag gggatgctat aggaaaagcc ttagatgaaa
    13681 acccagaaaa taaacattta caaaaccaac ttgttttatt aaataaatct agtattacaa
    13741 caatacactc attctgtttg gatgttataa aatcaaactt tcatagaata aacttagacc
    13801 ctaatttcag aataggagac caaactgaat gtgctatttt gaaacaagaa gcaatagagg
    13861 aagtatttga agatttatat gaagaaagag atgaggggtt tttaaattta gttgaaagtt
    13921 atgcagaaag aggaggagat aaagaagtac aagatattat cttgggaata tattcatttg
    13981 caatggcttc tcctgaacct aaaaaatggt tgattgactc agcagaacga tttaatattg
    14041 atgaaaattt tgatttttca cagtctattt gggctagagc tatattagac actgtaaaaa
    14101 tagaaattaa tggtctttgt ctaaatatgg aaagggcatt aaaagaagtg gaatcaatag
    14161 aagaattaga aacttttgca gaaaagttat ccgttgaata taaaaaaatt gctaacattt
    14221 cacaagcatg taataaatct tgggatgagg catataaaaa gatggcaagt atgtcctttg
    14281 aaaattatgt aaaaggagta aaaagaattt caaaagatgc tccttcgtat ataaaagaat
    14341 caaaagaaaa ggcaaaaact ataagagata agactaagaa gtctttagaa agtatagtaa
    14401 gtgctacatt caataaagat aatgattcta ttagagaaga aataaaatat ctttataaca
    14461 tagttaaacc aatatctagt gttgtactga gatttgaaga ggaatatagc aataagaaaa
    14521 gagaaaaagg aataatagat tttaacgata tagagcattt tgcattaaat atacttacag
    14581 atatagatga aaaaggtaat attgtacctt ctgatatagc cgttggatat agaaacaagt
    14641 tttatgaaat atttatagat gaatatcagg atagtaatct agtgcaggaa gtactgctta
    14701 aagctgttgc aaatacagaa actccaaata gatttatggt tggtgatgta aaacagagta
    14761 tatatagatt tagacaagca aagccagaat tatttttgca aaaatataat aactataatg
    14821 ataaaaaggg aagttcccat agaaaaataa tgctttacaa aaattttaga agtagagaag
    14881 aagttgtaga tgctgtaaat tatatatttg aaaatataat gaatgagaat ataggagaaa
    14941 ttgaatatac agaaaaagaa agactaaatt taggtgcgaa ttttaatgtg gatacagacg
    15001 agaaatctat tattggtgga gcaacggaga tacacctaat acaaaaagat aataaacttg
    15061 atgatgatat tataaatgat aaagacgata gaattaataa caaagaaaat gaaatagagg
    15121 aagaagaaaa attagataat atacaacttg aggctagaat ggttggaaac attatcaaag
    15181 atttaatgaa agtcaatgaa gatggaaaaa ttcaaaaggt ctatgataag ggaatagatg
    15241 gatatagacc tgtggaattt agagatatag ttattctttt aagagctaca tctgcatggg
    15301 ctccagtgtt tgcagatgaa ttgatgaata tggatatacc aacatatgct gatgttggag
    15361 tggggtattt tgatacgatt gaaataaaaa caatattgtc attacttcaa ataatagata
    15421 atccaatgca agatatacct cttatatctg tattaaaatc acctatattt ggatttacac
    15481 cagaggattt gattgatatt agagttcaga gtaaagataa aattttttat gaggttttaa
    15541 aaagtacagc agagtatgat ggatttacag attctcaaaa tgaaactgag tctgagttta
    15601 taccaagtga agagtgcata aacaaaagta aagatttttt aattaagcta aaggaattta
    15661 aagaaaagtc aatgtacatg agtacagatg aatttatatg gtacttgtac acaaggactg
    15721 gatactatgc ttatgttgga gcattgccag gtggaagtca aagacaagct aatttaaaag
    15781 tattgtttga gagagcaaaa caatttgaag aaaccagtct taaaggaata ttcaactttg
    15841 taaattttat agagaaatta aaaaaatcta gttctgatat gggaagtgca aaaacacttg
    15901 gtgagaatgc aaatgttgtt agaataatga gtattcataa aagtaaaggt ctagaatttc
    15961 cagtagtcat atgctctgca atgggtaaaa atttcaatac tcaagatttt aaaaagagta
    16021 ttttatatca tcataattta gggtatggac ctcaatttgt agactatgag agaaggattt
    16081 cttttccaag tatagccaaa gaagcattaa aaagcaaaat aaatatagaa aatttatcag
    16141 aagagatgag agttttatat gttgcattta caagagctaa agaaaaattg attataactg
    16201 gttctactag aaatatacaa gatagtataa agagatggtc aaatggaatt gaaagtttag
    16261 atacaatatc acaatacgag atattaaaag gtaaaaattt tttagattgg ataatgcctt
    16321 gtgtattaag acatagggat ttatctaatc tactagaaga agtaggatta gacgcagttt
    16381 ttaatgttga acataattca aaatggtatg gaaagttgtg gaataagaat gatattttag
    16441 tggaaaagaa gagtgatgaa gaaaaagaaa gtattgaaga aattcttgaa aaaatagatg
    16501 tagatagtcc ggacagtgat tattacagtg aaatagaaaa aaaattaaac tatatatatc
    16561 catatgaatt tagtacaaga aaacctgcta ctatatctgt tacagaaata aaaaagatac
    16621 aaaataatta tgaagaagag ttaataaata ctatatttga acaaaaagta atattaaaaa
    16681 agcctctatt tattcaaaat gaagaagaaa gagaaaaaat aagtggaaca gaaagaggta
    16741 caatagtcca tttggttatg gaggttttag acttaaaaaa tgtaagtagt gtaaatgata
    16801 taaaatcaca aataagaggt tttgtttcaa aaggtataat aacagaaaaa caagctagta
    16861 tagtaaatcc atataaaata tataaatttt ttgcctctaa cataggaaaa agaatgttga
    16921 atgctgaaat tataaataga gaaaaatcaa tttatgcaca agtaaatatg aaagacatat
    16981 atatttatga aaagcttata aacaatgatg ataaaaaact ttatgataat gaaagtgtca
    17041 tgttaagagg tatagttgat gcttattttg aagaagataa tcaaatagtt ttggtggatt
    17101 ataaaacaga ttttgtaaat gaggaaaata taaatcaaat aatagagaaa tataaaaaac
    17161 agttggattt atatgcagat attattgaaa ctttaactgg taagtcagta aaagagaaat
    17221 gtatatattt atttggagtt gatgaagctg tttgttatta gtttttatat ataaattaaa
    17281 cctaaaagat agaggaaggg gaaaaaatga gatttattca tacatcagat tggcatctag
    17341 ggaagtccct agaaggccat tctagaattg aggaacaagc aaagttttgt gaggaattta
    17401 taaaaatagt agaatcaaat gaaatagata tggttataat tgctggagat gtatatgata
    17461 cttctaatcc cccagcacaa gcagaaaaac tattttatca gactgtatca agacttgcca
    17521 ataatggtca gaggtgtgta ttaataattt caggaaatca tgataatcca gagagacttt
    17581 ctgcaataac acctctagca catgaacagg ggatacttat atatggttat ccattaagtg
    17641 ctacgataga agctaagtat aaagggtttg aaataacata tgcaactcag ggatgtacaa
    17701 aactgaatat aaatggagaa aatattgtga ttgctacatt accttatcca agtgaaaaaa
    17761 gattaaatga agttttttca agtgaagatg agtttgagaa acaaaaaaat tattctgaaa
    17821 aggtaggaga tatatttaga agtttagaag aaaattttag aagtgatact ataaatatag
    17881 ccgtatcaca tatatttgta ataggtggag agagtactga atcagaaaga cctatacaat
    17941 taggtggaag ttttttagtt gaaagaaaag atttgccaga aaaggctcaa tatactgcac
    18001 taggtcattt acataaacaa caaaaagctt ccgaacgttt aaatgcatac tattctggtt
    18061 ctccacttca atatagcaag gatgaaagag cgtataccaa aggggcatac atagtggata
    18121 taaaagctgg agaaaaacca ataattgaag atgtatattt taataattac aaacctatag
    18181 aagtatttaa atgtaatgga atagaagaag cactagatat atgtgagcaa aatcaagata
    18241 gagagatttg gtcatatttt gaaataaata ctgatgagat aatatcacag aatgaaatta
    18301 aaaaaatgaa ggaactatta aaagacataa tagaaataaa accaataata acatcttgtt
    18361 atgaacaaga aagtgtagat ataaaagaaa agtctatggc agagttgttt agagagttct
    18421 attcttttag taaaggtgta gagccaaaag gagaacttat ggatttattt ctagatataa
    18481 taagtgaaga aggtgaatca gcagatgaga ccaattagat tagaacttac aggacttaat
    18541 agctatattg ataaacaagt tatagacttt gaaaaattaa ttgaaagagg tttgtttggg
    18601 atatttggaa ctactggaag tggaaaatct acaatcttag atgcaataac tattgctatg
    18661 tatggaaata tatccagaaa tacaaaagaa tatataaaca gtgtgtgcga taaggcaatt
    18721 atatcatacg agtttgagat aggaagcaaa aacactagaa gaagatacat tgtagataga
    18781 accattgcta gaagcaaaac aggaacaaag acctctaatg ctagattaat agaagtattg
    18841 aatgacaata tccaaaatgt tttagctgat aaagttgtgg aagtaaatga aaaagtagca
    18901 caggttgtag gattaacggc aaatgatttt actaggtcag tagttttacc acaagacaaa
    18961 tttaatgagt ttctaagatt atcacgtgca gatagaagag acatgttaga gagaattttt
    19021 aatcttgaga aatatggaag aagtcttgga gaaaaagtga aaaagagaaa aaatatacag
    19081 ttacaaaatt taaaagattt gaaatcaaaa ttaagtcaat atgatggaat tacagaggaa
    19141 gtatacaaca atataaacca agaattaata gagttaaaaa atttggaaaa agataagaat
    19201 aatgctttag acctagctca aaaatcttat gaagaaagta aaacagtata tgaagaacaa
    19261 ttaaagttag aaaaaaatga acttagaaaa aatgagcttg atttaaaaaa tagtgagata
    19321 aaagaaaaga ttgctcttgt agaaaatgat agtaacgcta aaaaggtaga cccatacata
    19381 agttcagttc aaaatctaga aaaaaagata gatgaagata gctttgtagt aagtaattta
    19441 gaaaagaaat tagcaatatt aaatcaggag cttgaagtta caaaaaatag atatgaaaaa
    19501 ataaacaaaa ttaagaatga agaagtccct aaattgagtg aagaaaaaat aagattgcaa
    19561 caagcaataa aattagaaga agagttagtt ttattagata gagagttaaa agatctaaaa
    19621 gaaagtggaa ttaatttaaa taagactaaa gttgaattag aaaaagtaaa gcaggtgtca
    19681 gagtccagaa aagatgcagt tactaaatct ataaaagaag ttgaaggaaa gattgataaa
    19741 gtaaatataa gtgcagaatt aaaacaaaaa atatttctag catatgaata cgagaaagat
    19801 tacaacaacg tattggaaga aaaaaatcaa aagctaaata aactagaaga aatactcaaa
    19861 gagacagaaa atataaattt aaaagttaga tatattgata aagataagaa tgatgtaaat
    19921 cgtaatttag aaaatttatc acttcacttg gatgttcttc ttaaaaaatg tcctggtaag
    19981 agtgctgatt tacttttaaa atctgaatat gttactgaat taagaagtaa agcaaataat
    20041 actaaagaaa atgaaattaa gaaaagcagt atacaagatg aattaaagat aatacttgaa
    20101 agtaagttta atactgaaag ggaagtaaat ttactaaatg aaaaacttga aaataataga
    20161 aagaatagag atgacctaga aaaagaatta gaagaactaa agtatttaaa tttagcctct
    20221 gaacttagac gtgaattaaa agaaaatatg ccttgtccag tttgtgggtc aaaacatcat
    20281 gaaaatcata atataactaa gtatgatgaa aatatatctt ttgtcaaaga aaaactggag
    20341 aaattagaaa aagaaaaaat aagtataaga aacaatatag aggaattaaa tgctaaagta
    20401 agtgggtatt tatctataga aaaaatgaag actaaagaac ttgaagatgt aaaaggaaaa
    20461 ctagggggga ttccatctag tcaattatta aaaaaacttg atgaagaaca aagaaaactt
    20521 gcattgttaa aatctaatat tcaggagtgg gagaaagaaa aagagagtac tgaaaataaa
    20581 gtaacattag ctaaagaaga aaaaaataaa attgaaaaag aagaattaaa aataagagag
    20641 agtctgaata attataagaa attaacaaga gatttaaaca tagaaataga aggtttagaa
    20701 aataaatgta aaaaacttaa acaggaatac ctaggattaa agacgattac taaagtctct
    20761 aatctattgt caaaagtaaa agaaataagg gagaatgaaa aagaattaga attactaagt
    20821 actgggcatt caaatttatt aaaaaataga gactcactag atattaaaat aagagaaaat
    20881 gagagtaaac tacatgaaat agagatagaa ttaataaagg ctagagaatt atatgtagaa
    20941 aagaaattga gtagagataa taagtataaa gaagttatta acataacgaa aggtgattta
    21001 gctaaaaatc ttttacataa tgttgaagaa aatatatgta agatattaga acaagaagaa
    21061 tcaagtaaga aaaaattgga agagcaaaga ttagaatatg aaaaaaacat tgctgaaaag
    21121 cacaatatag atggtagact taaaactgct aaggaacaat ataaagacca aaaatcaaca
    21181 ctaaataaac tattagcaga aaataaattt gaaagtatat atgcagtaaa aagagcttta
    21241 ttagatactg atgcagtaaa aaaattgatt gaagaaatac tagagcatga agaagaacaa
    21301 aagttattat catttaagat aaaatcatca aaagaaaaat taaatggaag aagcataaag
    21361 aaggagtact ttgaacagtt aaaagatgaa atttataatt tgaaagtaga aataggaaat
    21421 atatccaaag atattggagc taatcaaaat cagttaataa cactaaaaga ctctttagat
    21481 aagataaatg attttaataa acaacttaag gtagttgaac acaatgtgga cttattggaa
    21541 gaattagata aatgtgtgca aggaaataaa tttgtagaat atgtttctac aaaccaatta
    21601 aaatatatag cattggaagc atcaaaacga ttggatggaa taacaaaagg aagatatgca
    21661 cttgaaattg actcaacact aaactttgta atgagagata actttaatgg aggagaaaga
    21721 agaagcattg atactctatc tggtggagag acattcttga catctctatc attagcactt
    21781 gctctttcat ctcaaataca attaaaggga agtgcaccat tagaattttt ctttttagat
    21841 gaaggttttg gttcattaga tagtgaactt ttagaaattg ttatagaatc cttggaaaga
    21901 ttgcatagta ataatctaag tgtgggtata ataagtcatg tcgaggaact taaaaataga
    21961 gttcctgtta agctattagt ttcatctagt gaggctggaa taggttcaaa agtaaaaatt
    22021 gaatatagtt agaaaatata tggtaattga atatattatc taatatttcc aaataatatg
    22081 taattgtgat ataataaaaa ctaatattaa agttatttat aattaatatt aatataaaaa
    22141 catgtaagta ggagtgtggt aagtgttgga atcgcccaat aatttgattc agattatttt
    22201 tttaatagta ttacttatag ggtctgcatt tttctcagca tctgaaacgg ccttaatgtc
    22261 gttgagtaaa atcagaatca gatatatgca agatgaagga gttaaaggag ctaagttagt
    22321 aagttcatta atagaaaatc caaataagct tttaagttct atattggttg gaaataacgt
    22381 cgtaaatata gcagcaacat ccatatcaac atctttgttt ataggattaa tgggagaaaa
    22441 aggagtggca cttgctacag ctgttatgac agtattagta ttaatatttg gtgagattac
    22501 tccaaaaaca atagcagcaa ataattcaga aaaggtttct ctattagtat ctaaacctat
    22561 aaaagcaatt atatttatat taagaccaat agtatggata tttaatataa ttacaaatat
    22621 tatatttaaa ttatttggta taacaaataa aggagctaaa tcattcataa ctgaagaaga
    22681 gctaaaaact atggtaaatg taagtcatga agaaggcgtc cttgaaatgg aagagagaga
    22741 aatcataaat aatgtttttg aatttggaga tatgcaagct aaaaatgcaa tggtacaaag
    22801 aatagatatg gtagctatag atatggaaga tagttatgat gagataatac aggtatttaa
    22861 aactgaaaaa ttaagtagaa tgcctgtgta tgaagaaact atagatgata ttgtaggaat
    22921 acttaatata aaagatatta tatttttatc tgatgaagaa atagagtcat ttgatattaa
    22981 aaattatatg agagaacctt ttttcacgta tgagttcaaa aagataacac aacttcttga
    23041 agagatgaaa cttgaaaaaa gtcagatggc tatagttgta gatgagtatg gtggaacatc
    23101 tggactttta actatagaag atttagtaga agtcattgtt ggagatatag aggatgaata
    23161 tgatgaagaa gaggatgaaa tacaagttat aaaagaagat gagtatattg tagatggaag
    23221 tactaaaatt ggtgatgtta atgaacttat tggtgtaaac ttggaatctg aggagtttga
    23281 ttccatagga ggttttataa ttggacacct aagcagattg ccagaagaaa atgaagtaat
    23341 tgaagttgat aatataagat tttgtataga aagtatagaa aaaaatagaa ttaaaaaaat
    23401 aagaatatat acataatgta ataatgttct taaatttatt agaagtgtat aaatataaaa
    23461 gatatctttt atatttatac acttcttttt attttgtatt caaataaaac tagtatgaaa
    23521 tggacaaaat aaaaatataa attagaagaa aggagatttt taagatgagc atactagaaa
    23581 ataatttttt atacaaatta aaaaacaaca ctatcttttt ttgtatatat acattgttat
    23641 ttatttttat ttacaaagcg tttccataca tagctccatt ttttctaggt gctataatag
    23701 cacttatgat taatcctata tcacaaaagt tagaaaataa gtttcgtata aataaaggta
    23761 tctctactct tgttctaagt tttcttgcag tggctatagt cagtacagtt actacaataa
    23821 tagtaataaa ttcaatgaaa gaattaatgg gatttttaaa taatatatct gctaatcctg
    23881 aggatataag taatactatt atgtattttt taaataaaat aaatgacttt atgaaatcat
    23941 ttcaagaaat agccaatttt gatgtagaac aattagtaaa caaattcagt ggtgaagtaa
    24001 tgcaaattac aaaaaatctt cttactagta tacttggatt ggctacttct attccatata
    24061 ttataatatt tataattacc ttgtttattg ctacctactt tatagcaaaa gaccttgata
    24121 agattgaaaa tagtttttat aatatgttta ctgttgatgt tagaaagaag gtaaaaaacg
    24181 taaagaaaga ggcagggctt tcacttgttg gatatataag ggcctataca atacttatgg
    24241 ctataacatt ttttgcaatc tggggaagtt ttgctttatt tggattgaaa tacggtctaa
    24301 tagtagggtt tgtaggagct ttaatggatt taattccatt cctaggtata ataacaatat
    24361 atcttccagt aattgtttat tattttctta taaaaaatta ttttatagct ataagtatga
    24421 ctgtgatatt ttttgttttg tctttgatta gagaaatact tgaacctaaa ctagtatcag
    24481 taaatgttgg actaaatcca ttagctaccc tagcagcgat ttttattgga atccaagtaa
    24541 aaggaataat tggagtaatt ttttgtttgg gtttggtatg tatgcatgat atacttaaga
    24601 aggtggatat tttttaatta taataattga tagatttttt taaaaataaa attttattca
    24661 caggtaaata aaaaaatatt aatcttatta agaaagaggg tatatacgtg aaacaaaaag
    24721 tagtggaaag atttttaaaa tatgtatcat ttgatacaac atctaattct cagtgtgaga
    24781 actgtccatc ttcagaaggt caaagagttt tagctaagta tatagttgaa gagctaaaaa
    24841 caatgggtgt tgatgatgtg agtttagatg aaaatagtta tataatggct actttaaaag
    24901 gaaatactga tggtgtggat actataggat tcatatcaca tttagataca attgaagatg
    24961 ttagtggaaa agatattaaa ccaagaataa tagaaaatta tgacggaaaa gacatagttt
    25021 taaatgaagc gttaaatgtt attacgtatg taaaggattc tccagagttg gaagaattta
    25081 aaggtgacga tttaatagtc actgatggaa caactctact tggctctgat gataaagctg
    25141 gtatagctga gatagtgact gcaatagagt atctaataaa tcatccagaa attaagcatg
    25201 gagatataaa aataggattt acaccagatg aagaaattgg tagaggtgct gatttatttg
    25261 atgttgaaaa atttggagca aaatatgcat atactttaga tggaggaata gttggagaac
    25321 ttgaatgtga aaattttaat gctgcaaatg caactattac tatacatggt agaaatgttc
    25381 atccaggttc agctaaaaat aaaatggtaa atgctataca tatagctgct gaaatatcag
    25441 aaatgttccc agcagatgaa agaccagaaa caacagaagg atatgaagga ttttggcatt
    25501 taaattctat aggtggaaat gtcgagaatg taagtatggc ttatattatt agagaccatt
    25561 gtagagaaaa atttgaaaat agaaaatcta ttatgataga gaatatagaa aaaataaata
    25621 agaaatacga taatagagtg gaattggatt taaaagattc ttattacaat atgaaagaaa
    25681 agattgagcc agttatgttt atagttgata tagctaaaga agctatggag gaattaggta
    25741 taaaaccacg tttagttcct gtaagaggtg gtactgatgg agcaagatta tcttttaatg
    25801 gattaccttg tccaaatata tttactggtg gattaaactt ccatgggaaa aatgagtgta
    25861 taccagttag ttctatggag aaagctacta aactaattgt aagaatagct gaaaaatatg
    25921 ctgaaagagt ttagttaaaa agtatttaaa aattaagcat ttaaagttat aatagctata
    25981 tcattcttta taagtcaagc tgattataag cttgtgttgt atttaaaatg gtatagctat
    26041 ttttgtaatt atttttataa aagataatta tttttagttt agggtatcaa taaaaagtat
    26101 ggatatcaat aggtattaac atttaaaagg gggaattgta ttgaaaatta aatctaaaaa
    26161 aataatagca tttgcacttt cacttgggat tatgttgcca agttgtgtat atgcagacac
    26221 tattagtaaa aatcaaaatg cacaggtaga tatgaacaat tatcttcaag gtagaaaatc
    26281 tccaaaggag gaaaaactta ttggtgaaga tagatttgat acagcgataa aaatatctca
    26341 aagtggttgg aataatggtt cagaaagggt gtttctagtt aactcaaatt cattgccaga
    26401 tgcattggct tcaactcctt tggcaagtaa attggatgca ccaatactgc ttacaaataa
    26461 aaatagtata ccatataatg taacagagga aataaaaagg cttaatccag cagaagttat
    26521 tttaattgga agtgaaggag ctatttcatc aagtgtaaaa gaatctcttg aagatatgga
    26581 agtattagtc agtagaatcg gtggagcaga tagagaagaa acatctttac ttcttactag
    26641 acagctagat gatactggtg atttaggtgt ctcaaaagtg gcagtggtta atgggtataa
    26701 tggactggca gatgcaactt caatatcaag cccagctgca agtgataata cagctataat
    26761 atatacaggt aaggatagca taagaagtga ggcaaagaat tttattacac aaaactcaac
    26821 taagacatac atagtaggtg gggagtacag tataagtaaa aaacttgaag gacagttagt
    26881 aaactcagaa agactagcag gaacagatag aaaagatact aatgcgaaag ttttagaaaa
    26941 gttttatgga aaatcaagta aggttaataa tatgtatttt gcaaaagatg gaagtggtag
    27001 agaagctgat ttagttgatg ggcttgctgc tggagtatta gcatcaaaaa caaagtcacc
    27061 tgtagtactt gcaagtggaa gtttaagtag tgcacagaaa agttttataa aaaaagtaaa
    27121 ggctgataaa tttgtacaag ttggtggagg taaaaatagt aaaccatatg cagaagcatt
    27181 agccttacag taatattgtg taaatcaaaa ttaatatata ataatagcaa gtaaaccaag
    27241 ttgaacttca atatatataa taaaataagt aaactaagtt atatttcagt ataatatata
    27301 atgaaataag taaactaagc tatgtttaa
//
LOCUS       DAMKPG010000046        27219 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMKPG010000046 DAMKPG010000000
VERSION     DAMKPG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.049"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            58..261
                     /locus_tag="PC621_002083"
     CDS             58..261
                     /locus_tag="PC621_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4109239.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            425..1579
                     /locus_tag="PC621_002084"
     CDS             425..1579
                     /locus_tag="PC621_002084"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF4109240.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1592..2596
                     /gene="rseP"
                     /locus_tag="PC621_002085"
     CDS             1592..2596
                     /gene="rseP"
                     /locus_tag="PC621_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF4109241.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2779..3834
                     /gene="ispG"
                     /locus_tag="PC621_002086"
                     /gene_synonym="gcpE"
     CDS             2779..3834
                     /gene="ispG"
                     /locus_tag="PC621_002086"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF4109242.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4182..5006
                     /locus_tag="PC621_002087"
     CDS             4182..5006
                     /locus_tag="PC621_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109243.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5479..6602)
                     /locus_tag="PC621_002088"
                     /pseudo
     CDS             complement(5479..6602)
                     /locus_tag="PC621_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6799..7023
                     /locus_tag="PC621_002089"
     CDS             6799..7023
                     /locus_tag="PC621_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109244.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7076..7600
                     /locus_tag="PC621_002090"
     CDS             7076..7600
                     /locus_tag="PC621_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4109245.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7705..7851)
                     /locus_tag="PC621_002091"
     CDS             complement(7705..7851)
                     /locus_tag="PC621_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109246.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(7990..8916)
                     /locus_tag="PC621_002092"
     CDS             complement(7990..8916)
                     /locus_tag="PC621_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF4109247.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9107..10978
                     /locus_tag="PC621_002093"
     CDS             9107..10978
                     /locus_tag="PC621_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF4109248.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11070..12005)
                     /locus_tag="PC621_002094"
     CDS             complement(11070..12005)
                     /locus_tag="PC621_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF4109249.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            12525..13037
                     /locus_tag="PC621_002095"
     CDS             12525..13037
                     /locus_tag="PC621_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF4109250.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(13192..14253)
                     /locus_tag="PC621_002096"
     CDS             complement(13192..14253)
                     /locus_tag="PC621_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF4109251.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(14377..15270)
                     /locus_tag="PC621_002097"
     CDS             complement(14377..15270)
                     /locus_tag="PC621_002097"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF4109252.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(15280..16761)
                     /locus_tag="PC621_002098"
     CDS             complement(15280..16761)
                     /locus_tag="PC621_002098"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF4109253.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(16869..17100)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17902..18810
                     /gene="trxB"
                     /locus_tag="PC621_002099"
     CDS             17902..18810
                     /gene="trxB"
                     /locus_tag="PC621_002099"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF4109254.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(18900..20723)
                     /gene="typA"
                     /locus_tag="PC621_002100"
     CDS             complement(18900..20723)
                     /gene="typA"
                     /locus_tag="PC621_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF4109255.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            20935..21177
                     /locus_tag="PC621_002101"
     CDS             20935..21177
                     /locus_tag="PC621_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109256.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            21325..21516
                     /locus_tag="PC621_002102"
     CDS             21325..21516
                     /locus_tag="PC621_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF4109257.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            21807..24308
                     /locus_tag="PC621_002103"
     CDS             21807..24308
                     /locus_tag="PC621_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF4109258.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            24468..25136
                     /locus_tag="PC621_002104"
     CDS             24468..25136
                     /locus_tag="PC621_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109259.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            25120..26397
                     /locus_tag="PC621_002105"
     CDS             25120..26397
                     /locus_tag="PC621_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF4109260.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            26798..27019
                     /locus_tag="PC621_002106"
     CDS             26798..27019
                     /locus_tag="PC621_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109261.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT        10559 a         3179 c         4368 g         9113 t
ORIGIN      
        1 atgaaaattt tccaaaatag ataaaatgtg atagaatata ttttgcgagg tgataacatg
       61 atttcatacg ctccattatg gaaattgtta attgacagaa aaataaagaa aatggaattt
      121 gtaaatatat caggtataag tatttctgtt ttaggcagac taggaaatga taaatctgtt
      181 tctatggata caatggagaa gatatgttta gcactagact gtaaaataga agatgttgtg
      241 gaaataaaaa agggcattta ataatactag aatattttat aatattccac aagatacaaa
      301 aaataataga ttaattttaa tctgtggaat aatatatgaa atttctatga atagtaaaaa
      361 taattgtttt agtagtgtta taatgttttg acagggtata tttattatta gaaaggatta
      421 ttatatgaaa aagatatcaa ttttaggttc tacaggctct ataggtaaac aaactttgga
      481 tgtggttaga gaacatagag ataagtttga gatagttgca atatcagcta acagtaatat
      541 tgaattattg ctagaacaaa tagtagagtt taaacctaaa tatgtaacag tttttgagga
      601 aaataaggca ttgaaattaa aagaaatgtt gcctaaaaat atagaaatag aagttttagc
      661 aggaatggaa ggattaaaaa taatatcatc acttgatgaa gtggatgtcc ttttaactgc
      721 tgtagtaggt atgataggat tagtcccaac tctttgtgct ataaagaagg gaatagacat
      781 agcattagcg aataaagaaa cgttggtaac tgctggtgaa cttgtaatga aagaagcaga
      841 aaaatacaat gtaaatattc ttcctgttga tagtgaacat agtgctatat ttcaatgtct
      901 aaatggagaa aataaaaaaa atatagaaaa gataatactt acagcatctg gtggtccatt
      961 tagaggaaag aaaaagggag aacttgtaaa tataactaaa aatgaagctt tgaagcatcc
     1021 aaattggagt atgggaagaa aaataagtat tgattcttca acgcttatga ataaaggact
     1081 tgaagtgata gaagctagat ggttgtttag ggtagaacaa gaaaatatag atgtagtagt
     1141 tcacccacaa agcataattc attcaatggt gcagtatgca gatagctcaa taattgctca
     1201 attagggtgt ccagatatga gactaccaat acagtatgct cttacatatc cagatagaat
     1261 ggaaagtagc tttgaaagaa tgaatttttc aaaatttagt actttaactt ttgaagaacc
     1321 agatttagaa acttttccat gtttaaaatt ggcctatgag tgtttaaaaa tgggaggaac
     1381 ttattcttct gttttaaatt cagcaaatga agttttagta agtgaatttt tagaggataa
     1441 aattggtttt tatgacatac catattacat agagaagact ttagaagttc atagtagcat
     1501 aagcgaacca acattagaag aaatcttaga aacagataga tggagtagag cttatgttgc
     1561 aaatctgata aaaaaatagg aaggtgaatt tatgactata atagccgcat taatactatt
     1621 tagtataatt gtattaatac atgagttagg acattttata tttgcaaaaa gaagtggtat
     1681 caaagtaaat gagttttcta taggtatggg gcctaagata tatagtgtaa aaaaagatac
     1741 agagtattct ataagagcac ttccaattgg tggatatgtc agcatggaag gtgaagatga
     1801 agaacaaata agtccaaatt cttttggaaa caagtctata ttacaaagat ttagtactat
     1861 tgtagcagga ccaatattca atataatatt agcagcaata cttttagttc cagtattttt
     1921 gtatataggt tctccaacga ctaaattagg aaagataatg ccagatactc ctgcacaggc
     1981 tgtagggctt caagtaggag ataagataaa taaaattaat ggtaattctg ttaagacttg
     2041 ggatgaagtt gcaaatataa taaacacttc ttctggtgga gaattaaaac ttagtataac
     2101 tagagatgga agtaataaag ttgtaaatgt aacacctaaa aataacaatg gaaagtatga
     2161 aatagggatt caaccacaaa gagaaaaaga ctttttggcg tcaatagtaa atgcatgtaa
     2221 aactactgta gatatgacaa aacagatgtt aacatttcta ggtcaaatga ttacaggacg
     2281 tgtaccagga gggattggca atgctgtagc aggaccagta ggtgttattg gtatggtatc
     2341 agatgctgct cgtacaggca tcataaatgt agtgtattta gcagctgtca taagcttaaa
     2401 tttaggtata gtaaatctat taccaatacc agcacttgat ggatggagaa tacttatgct
     2461 attactagaa gctgttagag gtggtaaaaa actcgaccca aacaaagaag gcatgataaa
     2521 tgttgttggt tttggagctt taatgttatt tatgcttttt atcacatata aagatatatt
     2581 aagattattt caatgattta gttttttaga gattttaaga aaataggcta tgtagtttta
     2641 aataagaata aattaatttt tatgtatgaa atttattctt atttaactag atagcttttt
     2701 aatttataat atacatgtag gttataatat tttactattt aaagagattc aatataaaat
     2761 aatatataga ggtggaatat gagttacgag agaagaaaga cgagagaagt aagtgtagga
     2821 actgtaaaaa taggaggaga aaatcctata agtatacaat ctatgacaaa tactgataca
     2881 agagatgcag atgctacaat agctcaaata aagagactag aggaagctgg ttgtgatata
     2941 gttagagttg ctgttcctga tattaaagct gctaagaata tagcaaagat aaagtctagt
     3001 gtaaatatac caattattgc agatatacat ttcgattata agctagccct tgaagctata
     3061 gaacaaggtg tggatggaat aagaataaat cctggtaata ttggaagtag agagagagtt
     3121 aagatggtag ttgagaaatg taaggaaaga aatttaaaaa ttagaattgg agtcaatgga
     3181 ggttctttag agaaagaact tttaaaaaag catggctctg caactgctga agcattagtt
     3241 gaaagtgcaa tgggacatat taagattctt gaagacttag attttcataa tatagttata
     3301 tctctaaaat catccgatat ttataaaact gttgatgcat atgaattaat atcaaaaaaa
     3361 gttgattatc ctcttcatat tggaattaca gaatctggaa gtgttcacaa aggaacaata
     3421 aagtcttcta taggggtagg agcacttctt cttaaaggaa ttggagatac tgttagaata
     3481 tctttaacag gcgaccctgt tgaagaggtt attgttggta aacagatttt aagaagtttg
     3541 ggacttttaa atgataagat taaagttata tcatgcccta catgtggtag atgtaatata
     3601 gacttaatta gtgttgtaaa tgaggttgaa gaaaagattg gtagtatgga aaaaaatatc
     3661 actgttgcaa ttatgggctg tgctgtaaat ggacctggtg aagccagaga agcagatata
     3721 ggtattgctg ggggtaaagg tgaaggtctt ttatttaaaa agggtgaaat agttagaatg
     3781 attgatggta acaaattagt tgatgagttg ctagaagaga ttgaaaaatt atagtataca
     3841 attcatatat atattaattg actagatttt taaatacttt tgtaataagt ttttatttac
     3901 agtatttaat tatctagtca ttttttattt tataaacttc attaaatgta agaaacttat
     3961 attctaattt gactatatat gtatgagtta aatcatattg taaatttata aaattaaaaa
     4021 aagatttaat atagtgaaaa tttccatcaa tagtattgaa acttgatagt aactatgata
     4081 ggatatagtt actagaacag ttggtaaatt atgttaacat agattttaat gttttggtaa
     4141 attgttttat tataagttaa tagaaaaaga ggtgaagaac tgtgagatat aaaaaaatat
     4201 ctagcttacc tattttagtt gttgctttgc tgattactgg aacgacaaca acattttctc
     4261 ttagtacgac caattcagaa aaaacaaatg tatacttagc aagtacggta agtactgtca
     4321 caaagaaagt atccgatatt caaagtagta aaggtcttgt ttataatgag aatattccat
     4381 tatttataaa taatagcaaa atacagtatg atgatactcc atatcattgg cctagcaatg
     4441 ttataagctt aacaaatagc agtgaaaagg caattatgga ttatgagatt acatgcttag
     4501 catatgataa aaatggaaaa ccacttgagc tatattggga tgctcaaaat gtggcagcag
     4561 atggcgaagt aggaagtgtt ggatttagcc cagctggtgt agactatggt attgttacag
     4621 gcatatctcc tgtgtctcca aaatcatatt cacatactta tagaaaaatg caacaatcac
     4681 caccacagga tataataagt atgtttgaaa aacaacaagg caaggcatgg gtagaaaatt
     4741 ggttgaaaga atggaaacaa atggagaaag aatatgcaaa gcaaaatgca atagcaccag
     4801 gtaagaatca aaatgatgcc tttctattgt ttgataaatg gaaacaatca actggagagc
     4861 atggtgtaaa gtacattata tcttgtgtaa aacaagtaac atttaatgat ggtagtatat
     4921 ggaaaaattc agcatatgag aattggttga aaagttttca agggaaggaa gtttcaaata
     4981 gtgtattaga aaattactat aagtaatgtt taatgatagc aaaatgagta cgaatttaat
     5041 taatgttaat ttaattaaat aaggactatt ttgtttaaaa atatcagtgt tacaaggaaa
     5101 tttttattgt gacaaaatgg tatatattca aaataaaaaa gcatgtgtca agttttgata
     5161 catgcttttt tattttgaat atatgttttc tataaattta tgatttttta gttatgtaat
     5221 tttatatgtg aatttttaga tttttatata gatagtgaaa taatgtggaa gttttatgtt
     5281 ttaaaaattt tagttaaaac atttattaaa aaagataata gaaatgtttt aactaaaata
     5341 gttagaattg aaatgttata cagatagtat aagtactatc aaattatata agattgcttg
     5401 aagttaaata atttatttaa atagaaaaca gctgatttaa tatcagctgt atatgaataa
     5461 aattttgaat tgtagtgttt agttatatgg atataattca taataagtta gcaataaatc
     5521 aaccatttta ccataactct gagtaccttc agtaacgcca ttagctttta agtaagaatt
     5581 gttaaactca ttagaaattt cattaatttt accttcatat ttttgccaaa attcactttc
     5641 attttttaaa tctctacgga cagaatctga aatgccagca gaaatatcaa catatgcatt
     5701 ataatcaacc ttacttaaag cagaagcagt ataatttaaa gctaaaatat atccagaata
     5761 gttaaaatca atatgggggt gttttataga agtaagatac gctataaaat tagcctcatc
     5821 ttcactagca aatccccttt gatgtgctat ttcatgttca actgtacaag gaatatataa
     5881 gtcaggtatt gctatattta catttgcctc acctgtaaaa ggaaagtata taccagttat
     5941 tcctgtatag cacattagat ttgatgaaat aatatactta ggcttagaat aactaccact
     6001 aacactaggt aatatatcca agatattatc gtatcctagt tgagctctat ttatgacacc
     6061 attataatct gtattagatt ttacaatacc atttttatct tgtaatgtga gttttctagt
     6121 ttcatttgct ttaattacaa ggaatttata taatttagta agctctttaa cactatattg
     6181 ttttgattgg atagaggtat tgttttttaa attatagtta tttataagtg tggtttctag
     6241 tggaatccta ttatagttta ttccccaaag cactacaaaa agaaagtaga ctattgatac
     6301 aatggataat atatttaaaa tggaattttt taaaaatatg tttaattttt tttcttatta
     6361 aaaattgtag ataaagtaca acataaaaat aagaagatag atataactat tatgtacatt
     6421 gtaatctcat agagagaaaa tgggaaaatt ccagaaactt tactcaatat ttgtacaatt
     6481 attttatcta tgaattggga gtaatatttt tcaacaatat ttggaacttt gctggctata
     6541 aaatttaaaa gtaaagcaat aggaaagaga attaaagaaa aatattttgt ttttttgctc
     6601 atatttcacc tcacatacat tagtttatat aaacatatta tatatcaatt gaagaaagat
     6661 tgtaaaataa gtataaatat atctatttat aactttatac taaataaatg ataagttata
     6721 atatattaat atgaataagc agtatatata caactaataa taagaaaaat atcaattata
     6781 taaggagtag ataaaaatat gataataaaa cttactaaag acaattttaa ttttagattt
     6841 aaacaatatg aaggtatgtt ttttcatgct gaagtagttg aaggtatgga atccattaga
     6901 tataaaataa ctaaggataa agaaggtaca gaattaaaag aatttggaag tacacatata
     6961 aaagataata tgatatatgt acctcaggta gaagcataca tagatttgaa cagtataaaa
     7021 taaagatgta aaattttaat taaaaataaa gattatgttt gagggggaat ccaatatgag
     7081 taagcctata gatataacaa taagacctat aagaagagaa gatgcacaag cttttaatga
     7141 aattagaaga acatacagtg ttatgagaaa tactttggca ttaatgagtg acagagtcga
     7201 taaaactgaa gctatgttag cttctttggg agaaaatgat tatatgtttg tagctgaaac
     7261 taataataat ggagaaaaag atgtaatagg ttttgctggg ttacatgtaa attcttctcc
     7321 acgattgaga catagtgcag aaataggaat aagtgttgca gagaattatc agggaagagg
     7381 agtaggtaag aagcttatgg aacaactttt agatatagca gataattgga ttatgttaac
     7441 tagagtagga ttagaagtaa ttgtagataa tgaaaaggga ttaaatttat ataaaaaact
     7501 aggttttgaa atagagggaa ctaaaaaata tgctgtaatc agagatggaa aatttgaaga
     7561 tgtttatatt atgggaagat ataataaaaa tttaatttaa agaagctaat agagaagtaa
     7621 aatagagtgt ctttaataaa aaatttacat taaaaataat ctattttact tctctattgt
     7681 attaaaaaac ttatttttaa atgtttacaa aaacattcta tatacaaata aaattattag
     7741 caatatcaac gctattttaa aagctaaact agcaacagtt ttaatgattt ttacagaaac
     7801 acctatagca actatagcaa atactatttt aaagagtaaa tccatatcca tatttaagcc
     7861 tcctattcta taatacatat agatattatt gacaaatatc taggaaaaaa ttccatcaat
     7921 atactacaat aaaatgttta tatttgtaat ttattgaata tgttattata taatacataa
     7981 aaaaataaat caagataaga gtaaaatact tataaaaggt atagatataa ctgaaaatag
     8041 tgttgtaaaa aacacacatt gtgaagctag tgttatattg gagtcatatt gattagccat
     8101 tatagcagta tttgcagcag caggcattga agaaattact actggtatag ctaaaatcat
     8161 cttatcattt atccatcctt ttagaataaa atacacaatc acaggaagta ccaataatct
     8221 tataaaagtg actatatata attttttgtt gacaaaacaa tcgagagctg aagaattagc
     8281 aagtaagctt ccaatgataa tcatggatat tggtgtagtt atacttccca acatctcaag
     8341 tggctcgttt ataaattggg gtaacctaag tcctgttaca aataaaaaaa gtccaataac
     8401 aatagctatt gtagcagggc ttataagtga ttttattgaa aaacctctac ttgtactgcc
     8461 tttagataaa agataaattc ctaaagtgaa agaaaataag ttaaaaggca aattgaatat
     8521 tgcagtatag aatattgctt catgacctaa aactgcttca attacaggat aacccataaa
     8581 agcaacattt gaaaaaacaa ctatatactg atatattccc aaatcttttt tatcattaca
     8641 ttttaaaatg tatttcaata aaaatgcaat tataattgaa actatataca taatcaaaga
     8701 tgtaaataat aacaatagta ttttttgtat cattttacta tcaaagttaa tttgcatgga
     8761 gctaattatc attgatggta aaaaaactgt cattgtcaaa gttgaaagct tagatgtgca
     8821 gtggtcatct aataagttga attttctgac aaaatatcca actaaaataa ttataaataa
     8881 aactgcaact tggataaaaa tattgctgaa attcataaaa tgtccccccc tatatataca
     8941 ttatatacca attacataat aaaatctaat gattataaaa tttaaaattt aatgttacaa
     9001 tatagtgaca aaaggtaaaa attcaatatt atagcattaa ttatagatta taatgtataa
     9061 taatgtttga gagaatatat tttataaaat gggtaggtag cgaataatga aaaatacaaa
     9121 attttctata aaaaaaataa aaaatatatc taatgacccg aaagtcgtac atactaaggg
     9181 tgccctcctt gaaaagatgg gtaagatgga aagcgcaata gagttgtata agagtgctgc
     9241 attggataat tacgtaaaat ctcaatatac tttaggaaat atatatgaaa gtaaaaaaca
     9301 atttaaagaa gcagaaaagt ggtattcaat ggcatataaa agtggaagtg aagatgctgc
     9361 ttttgattta ggtaatatgt actataagtt agatggctat gaatatgcta tatattggta
     9421 tgaaaagata gcaaatttag gttatttaca agcacaaaat aatttaggtg tatgccattt
     9481 taaaatgaaa gattttgtaa ggtgtgaaaa atggttaaaa acagcagcag acaagaattt
     9541 aggtaaagct tgttttaatt taggggtatt atatactttt ttggagaaag atgatgaagc
     9601 atatgaatac tataaaaaag gttctgtgct tttagatgat gatgcaaaat ataatttagc
     9661 aatattaaat ggaaaaaaga aagataataa atcaacagta agtttatata aacagttgta
     9721 caagtcaggt catatgaaag gttgctttaa cttgggtatg attatggaaa taaatgataa
     9781 tttagaagat gcagagaagt attataaaaa gagtgctgat agagatgatg tgaagtctga
     9841 gtataggctt gcatatgtat atgatagaaa agaagagctg ggagatgcaa tttattatta
     9901 tgagaaagca atagaaaaaa atcatacact ttctaagtat agacttgcta atttatataa
     9961 tagagaaaat gagatagaga aggcaaaaac ttattataaa atggctgctg aagatgatat
    10021 aacagaagca aaaaataatc tggcaggtat atattttgaa gaaaaagatt atgaaaatgc
    10081 tattaaatat tatgaggatg ctatagctgt tggatgcaaa agttcccttg aaaatcttgg
    10141 ggatttatat tatcagaatc aagatataga aaaagcgatt tcgtattatt cgaggattcc
    10201 aaataatgca tcttgtcaaa tcaaattagg aaatatctat gaagatttaa ataatattga
    10261 agaagccata agttggtata agaaagcatc tgaaaatgga gatacgaggt ctagttatag
    10321 attggggtgt atatatgaga gcttaggaaa tacaaaaaat gctagaaagt attttgaaat
    10381 ggcaagtagc aaaaatcata tgaatgctcg tatacatcta ggtagaattt attttagaga
    10441 aggtaaattg gaagagtcta aaatgatgtt tgatacacct gccaatgaaa ataatgtgta
    10501 cgctcaacat atggtaggat taatttatga tatgttctat aaagattatg taaattctaa
    10561 attttggtat gaaaaggcta gagcacaagg ttgtgttgaa tcaatatata atttaggcca
    10621 aatttattta aaattaaatg atgacgcaga agctgagaaa tattataaag aaggtattaa
    10681 atatggaagt aagaagtgtg agtatatgct tgcaggtctt tattataaaa agagcctaga
    10741 tatgtacatt tctttagcaa atgaagagta cgataattgt gaagagatag tagaaggctt
    10801 tcccaattta aatatagact ttgatgaagt attaattcct ccatttaagt tgcaagaaga
    10861 ggtaatagat gaagaagagt atgtaccaat ttatatacta aatataaaag aaagtttaga
    10921 ttcaatgcta gaagggcttg aaactgaaat gattatagat gaagaacatg atagttaaaa
    10981 agtattataa gattatatag atttaaatat ataaaagcca ttaggataaa tgtaaatcaa
    11041 ttaattctaa tggctttttt attaatattt tataatagct tatttcttga catgaaaaat
    11101 gtaatcacta tacatattat agaggtaata agaagtatta tccaaaatcc gtttgggtta
    11161 ttagaaaatg gcatgttgat gacattcata ccaaagaatc ctgatattat tgtaggtatt
    11221 gagaaaatta gtgtaactac tgttaaaatt tgcataacat tattctgatt attacttata
    11281 attgcactga aagcatccat aattctactc aatatatctc catatatttt agccatatca
    11341 agagcctgtc tattttcaac tataacatct tctaataggt cttcatcatc tggatacttc
    11401 tttataccag cagttctaac cattttgttt aaaacaagtt cattagattt tagtgaggta
    11461 gagaagtata ccaaagattt ttctaattcc aataattgaa ccaattcttt gtttttcatt
    11521 gatttgtaaa tttctcgctc tattatgatt gtcattttat ttatttttct taagtaatgc
    11581 aagtaataag ctgcattttt gtgaagaatt tgaagcaaaa agcgagtctt tttaaacgta
    11641 aaaaaatctt taatatgacc aactataaag tcatttaaaa tcttagtctg tgcatcacaa
    11701 actgttacaa tagcttcttc agttagtata atacctaagg gtattgtagt ataatgagaa
    11761 gaattagagt cactttcgtc tactggtata tctataagta taagagtatg attgtcttca
    11821 acatctattc ttgagcgttc ttcttcatct agtgcagatt ctatgctttc tacatctata
    11881 tttaataaat tagaaatttc gttgatttca ctatgattag gttcaacaag atttatccaa
    11941 cagccatctt caaaaaaact aagtttctct aattttgaat ctatagtttt gtaatatctt
    12001 atcatggcaa cacatccttt attcagtttt atataaatct catcatttaa tagtatctat
    12061 taattaacta atttatataa ctgaatatat gtattagttt tgattttaga tattattaag
    12121 tgatgaaaat gtaaaaaatt ctctccgcaa cggactagta tccattgccc atcactctcc
    12181 ttatttatta ttttatatat agctatttat gaaatgcgta ttttaagcac tctttaatat
    12241 aatacctttt ccaggtatat aaatcaagtt ttgatttgat taaataatat ttttaccata
    12301 atttacacat aaagtatata agttatattt aatttgagta aagaatatat atattctgag
    12361 tgaaattatt aaatatatta aaaaataaga gtgtatatat atatttactt atttgcaata
    12421 atatatttaa aaaagtattt tattttcaaa actagacttc atttataatt tatcacgaaa
    12481 ataaaaggaa tataaatata agagaatgag aggttattag agatatgagt gattttaaag
    12541 aaattttggc agaagagatt aaaaataatc catttggatt gataggtaaa gattggacac
    12601 ttataacagc agaaaatgaa ggtaaagtga atacaatgac tgctagctgg ggaggacttg
    12661 gagttatgtg gggaaaagat gttgcttttg tagtaataag accacagcgt tatactaagg
    12721 aatttataga taatacagat aaattttctc ttacattttt tgatgaagat tttcgtaaag
    12781 agctttcata ttgtggaaag gtatctggta gagaagagga taagatatca cagattggat
    12841 ttaatataga acacttaaat gatacacctt attttaagga agctaaaatg gctattatat
    12901 gcaaaaaaat ttatagtcag aaacttgaac cccaatgttt tattgctgaa ggtatagatg
    12961 gaagatggta tccacaaaaa gattatcata ctttatatat agctgaaata accaatgtac
    13021 ttgtaaaaga agattagtta taagggttaa tactatctct aattaaatta aaatttcaat
    13081 aaaaggaaat tgacacaaaa aagctaagga cataaatatg tttataaaga ttaataaata
    13141 acgtattaaa actccttagc ttgataaaat tttaatttag ttttctttaa attataattc
    13201 agaaatcaat tcattgataa catcttcaac agtacttaat ttatcaattt tataggcatc
    13261 atttccacaa aataaaagtg aattttcaac atctccttta actgcattga ttagagcttg
    13321 agaaatacag taaggagttt cttttggatt acaaggaatc aaacaattat agcacttctt
    13381 tatttctgga cgatttattt taacttcttc tataaatttg tttcttatgg cgcgtccagg
    13441 aagacctaca ggacttttaa ctatatgaac atcttcttca ctagcattta tgtatgccat
    13501 tttaaagttc tcatgggcat cacattcgta agtagcaaca aatcttgtac caacttgtac
    13561 accacttgca cccatatcta tatatttttt aacatcacta ctagaagtta taccaccagc
    13621 agcaactact ggtatgtttc taccaaattt ttctccataa gtatttgcaa cctttaaaat
    13681 ttcaacaaat tccttatcat agtctataga atctatgtta tcaagttcct cgttagagta
    13741 tccaaggtga ccaccagctt ttggaccttc aacaactatt aagtcagcag tagttttctc
    13801 ttttctatcc cacattttta aaattacatt agcagctttt gcagttgaaa ctatgggtgc
    13861 tatttttaca ttacttccct ttactaatga tggtagctta ttaggtaatc cagctccaga
    13921 tattataagg tctacaccag ctttaacagc ttcctttacg tatgtttcat attctttcat
    13981 agctaccata aaattaatac caataatacc accatttgag atttctttgg ccttagaaat
    14041 atgttttttt atagctctta aatttgcatt tatagtatta gtttcaaaat cttcctcatc
    14101 atatccaatt tgaactccag aaattacacc aacaccacca agcttagcaa ctgcaccagc
    14161 tagtgatgac ctagatatac caacgcccat tccaccttga attataggaa cttttgcaac
    14221 taaatctcta attattaact cttttatgat aatccctcct caatgtaaac atgtttaaag
    14281 aactagatta taatacttaa tattattacc tagtcctaat tattatacta caaattataa
    14341 taaaagagta gttatatttt gtatagaaga cgataattat attatattta aaacagctcc
    14401 attagaatcg caacataact cttttatttc ccaatcagat tttagttttt gtaggtaatt
    14461 tttcatatta tccacaaaac tatcatcatt ttcttttatt aaagacatta tagtaggtcc
    14521 agctccactt aaaaatactc caatactatt taattgttca gatttttcaa caatatcatt
    14581 gtagttctca attagagttc ctctataatt ttgatgcaat ttgtcttgac aagctacttt
    14641 aagtaggtca aagtttttgt ttagtaaggc agatattagt aaagcaactc tactaacatt
    14701 gaatacccca tcagaatatg gtatttcctt tggtaaaaca cctctagctt tttctgttga
    14761 taacttaaaa ttaggtatca ttgcacaaaa tttaagttct tcaggaattt ttataatatc
    14821 ataatgtata ttctcattat cagtaacaga tacgatcata tttcctaaaa tagcaggagc
    14881 aacattatct gggtgacctt ctatttctga agctatattt aataattgat ttttatctaa
    14941 attagcacct gatagagcat ttgcagctat aactccagct actatacaag aggcactact
    15001 gccaagacct ctacatatag gtacttcact gtggattttt attttgatac cagcaggtat
    15061 tttttctggt ttaactctat caaaaaagta cttcatagat gtataaacta agttattttc
    15121 gtttttgtaa gcatcttcac aaccttctat ttcaagacca ctttcaattt cttcaacata
    15181 aaatttattg tatatattta acgcaattcc aagacaatca aatcctggac ctatattagc
    15241 acttgttgca ggaactataa tctctaacat aataatcacc tataccttta agaatttttt
    15301 tatagctagt tttaattcat ctttttcata aatatcttta tgtagtattt ctgcattttt
    15361 aagattttta ataggttttg gaatatctat tttagaaata cttgataatc tatcaattat
    15421 agcaaagtca tcaaggtctt taaaatcaca atctatagac tttagaacat cttcagaaaa
    15481 cttgaatgga cttgcagtac ttacaataac agtttttgtg ttatcagaag tttctttttt
    15541 atatttttca taacaattgt atgcaacagc agtatgagta tctatcaaat aattataatt
    15601 tttaaacaca ttgtttatcg tgcttttaac ttcttcttca tttgaatatt ctccatagaa
    15661 tgaggctaaa tttttttcca tatcatcact tattttgtag actcctttat cactaaggtc
    15721 tgataacagc ttatttacaa tatcagtatt tctatttgat atttcaaaca gtaatcgttc
    15781 taaattactt gaaattaata tatccataga aggagaagta gttaatttca atgttctatt
    15841 tttatcatat acaccagttt taaagaaatc ataaagaaca ttattatcat ttgatgcaca
    15901 tatcaatttg tttattggaa gacccatttg tttggcataa tatccagcta gaatattacc
    15961 aaaattacca gttgggacaa caaaatttat tttatcattt aaatttatct cactattgct
    16021 aactaatttc atatatgaat aaaaatagta tgcaacttgt ggaagaagtc ttcctatatt
    16081 aattgaattt gcagaagata atatataatt attatccaat agtttttgat taaatttttt
    16141 atcagaaaaa atcttcttta cacctgattg tgcatcatca aaatttcctt ttataccaac
    16201 tacatatgta ttttttccag tttgagtttt catttgaagt ttttgtactg ggcttacacc
    16261 atcttctggg aagaatacaa ttatctttat tttatctata tctttaaatc cctcaagtgc
    16321 agctttgcct gtatctccag atgtagcagt aagaatcaca acatctttct ctaaattatc
    16381 tttctttata gatgttttaa gaagatgagg cattatagta agtgccatat ctttaaatgc
    16441 taaagttgga ccgtgatata gttctaagaa ataagtatca tttactttat ttaatggagc
    16501 tatactacta caatcaaact tttcatcata tgcattttct atacaatttt taatttcatc
    16561 ttcagtaaaa tcacataaaa atttactaag tacaaaaaat gctatctgag aatacgttaa
    16621 atttactaaa tttataagtt cattttttac attgggaaat tcacttggaa cataaagacc
    16681 accatcactt gatattcctt ttattattgc ttgtgatgca gttacttttt cttcggtgcc
    16741 tctcgtactt gaataataca ttttttataa tcctccatat attttaaatt tttaattagc
    16801 taattaaatt agatacagaa tattttaaaa accgaattta ggtaataaaa aaagaccctc
    16861 atccctaaaa aagggacgag aatctgtttc ccgcggttcc acccaaattg attttaaaaa
    16921 atccacttga tactttaacg caagtaataa aaacgtctat tcctactaaa atcattaaga
    16981 taacttcgga tagaagctcc taggtagttt tcaatccttc taattaggga aacatttcag
    17041 ctagtagttt ccttctctgg taatcgattc agatttactc ttcctaatca atgcctttca
    17101 atattcttaa tattattaat tattctagtt tataaaacag acaaagtcaa gtattaatat
    17161 aaaaatttgg aaaaaatatt ttttaaattg taaaaaaatt gtaatcaaat tgagtattag
    17221 aaaaatattt ttttaacttt tttaaaagtt taaaaaccta gattttcata gtgatgaagc
    17281 tgtttttaat gaaagtaatc aaaagataac tattaaatat ctgtaatatt acaattttat
    17341 actacactat attacaaaaa aagtttacaa aactttaatt tgaaaaatgt aattatatgg
    17401 tatatcatat aaatataagt aaatagtgct aatatttaaa ctattaatca taattggttc
    17461 aacaaaagta tatagttaat aatgtttaat ttgaagaaat tgttagtgtg aaattacata
    17521 tatattcaaa ttaaattcat aaatatttca cataataata gtatactgta aacaaaagga
    17581 ggtattaaat atgggaatag tacttataac agcagcatta gttttggtta tgtatggact
    17641 ttcaattcta tcatatatat cagaaagcaa tatattagaa gacactgtaa atgataacca
    17701 ataaggtttt gaattatata aataagtgag aactgtaaat aaaagttaat ttttatttgc
    17761 agttttttta tatatatgta aaataaagtt aattaataaa aaataatgtt ttgtttgtag
    17821 tatatatgga taataatagt aaagtataag aatgatgaga ataatatata agaaataata
    17881 atataaagag aggtaaacat tatggtagat atcattgtaa ttggtgctgg accagcaggt
    17941 ttaacttcag caatttatgc tatgagagca gggttaagcg ttacagtatt tgaaaaaaat
    18001 atttatggag gtcaagttgc aagtacctct gaggtagaaa attatccagc agttcaaaag
    18061 atttctgggg tagaattttc taataatata tacaatcaag cagttgctca aggtgttgat
    18121 attcagtttg atgaggttga agagataaat ttagagggaa aagtaaaggt tgtaaaaact
    18181 tcttcaggtg aacataaagc aaaggctgtt attcttgcaa atggagttga aagacgtaaa
    18241 ttaggttgtg caggggaaca agaatttaca ggtagaggag tatcttactg tgcaacatgt
    18301 gatggagcct tttttaaaga taaagaagtt gctatagtag gtggaggaaa tactgcacta
    18361 gaagatgctt tatttttagc taataattgt acaaaagtgt atttaattca cagaagagat
    18421 agttttagag gtgaagaagt gttggagaaa tctgtgaaag caagagagaa tattgagata
    18481 ctttacagtc atggtgtaga aaaaatagaa ggagaaaaga ctgtatcgaa aatagaagta
    18541 aaaaacttaa agactgaaga aaaaagaact atagatgtta gtggtatttt tattgctata
    18601 ggtcttaaac ctaataataa aatgtttgaa aatgttcttg atttggatga aggtggatat
    18661 ataatttcag atgagtcttg tacaacatca gtagaaggtg tatatgtagc aggagatagt
    18721 agaactaagt ttttacgtca aataataaca gcagcatcag atggagctat tgcagccgtt
    18781 caagcagcta attatataaa tgttgaataa tataaatttc aaataaaaat agccatataa
    18841 actgaaataa tttttcagta ctatatggct attttgttaa ttagaggtaa atgtacgttt
    18901 tatttacctt gtctagattt gttatatctt aatctatcat gttcatttaa gaatcttttt
    18961 ctaagtctta tagaatcagg tgtaatttca actaattcat catcttctat aaactctagt
    19021 gcttcttcaa gagtaaatat tctaggagga gataatttta tagcatcatc tgaaccagaa
    19081 gcacgtacgt tactcatttt cttgttttta caagggttga ctaccatatc atcttttctg
    19141 gaattcatac caattatcat accttcgtaa acttctacac cagggtctac aaacattaca
    19201 gctctatcac ttaaggcatt aagagaatac cccatagtaa caccaggtcc ttgtgctatt
    19261 aaaacaccat ttcctcttga aggtatttct cctttaaatt cttcaaattt ttcaaatgaa
    19321 cgaactaaag taccttctcc acgagtagcg tttataaatt cacttcgata tcctaaaaga
    19381 ccacgagtag gtgctaagaa ctctattttt acatagtctc cttctattga catagactcc
    19441 atcatacctt ttctcatgtt taattcattt ataatagtac cagagtaaac ttcaggacag
    19501 tttacaacaa ctctttctat tggttccatt agttttccat cttctttatg cattaaaact
    19561 tcaggtttag aaacaccaac ttcataacct tcacgtctca tattttcaag tagtatagat
    19621 aagtgaagct ctccacgtcc agaaactttg tatccatcag tagtatcaag aggttcaact
    19681 ttaagaccaa catttacttc aagttccttt tcaagtctat ctttcaaatg tctagttgta
    19741 acgaattttc cactttttcc aacgaatgga gagtcgttta ctaagaagtt catagaaaga
    19801 gtaggctctt ctatttttat catttccata ggtagtggac tatctaaatc gcatatagtt
    19861 tcacctatag atatatcagg aataccagct ataacaacta tttctccact agtagcttca
    19921 tcaacttcaa cttgttttaa accttcatac acagaaagtt ttgatacttt tcctttagat
    19981 acaacagaat cttctctaca tatagcaact tgtgtattgt ttttaagagt acctttgtaa
    20041 attctaccta taccaagtct acctacataa tcatcatatg caagagctga tatttggaat
    20101 tgtagaggtt cattatcgta attaggataa gcttcaacgt gatttacaat agttttaaat
    20161 agaggagaaa ggtcttcact atcatcatcc atttcaagtt tagcaatacc ttgtttagca
    20221 ataccatata ttataggaaa ttcgcattgc tcatctgttg catttaagtc tacaaataaa
    20281 tcgaaaactt cattaactac ttcttccgct ctctggtctt ttttatcaat cttgtttata
    20341 aataatattg gttttaaacc gaactctaaa gatttttgta aaacgaatct agtttgaggc
    20401 attgggcctt cactagcatc aactaataga ataactgtgt caacagtttt cataacacgt
    20461 tcaacttcag aagaaaagtc actgtgtcct ggagtatcaa ctatgtttat tttgtaatct
    20521 tcatagttta ttgcacagtt ttttgaatat atagttatgc ctctttcttt ttcaaggtcg
    20581 ttactgtcca taacgcagtc tttaactact tcgttctttc taaaaacacc gctttgagaa
    20641 aggaaagcat caaccaatgt tgattttcca gcgtcaacgt gggctataac tgcgatattt
    20701 ataattttat gtttttgtga catttttgtt gctcccttcg attaaaataa cttattaatt
    20761 atagcacaat atattattag gtacaaggag gaatatatta aattgtatta atatagttta
    20821 aattggataa aactgttagt tttgcatctg aaaattattc tgtttaaaaa aattttttag
    20881 acaaataata atcttaatag ataaaaagat taataaaaga gtaaaggatg atatatgttg
    20941 gaagtaacca aaataataat taaatatttt gcaagtgaaa gtgatacttt tataaaagaa
    21001 gaagtttgta attttgaaga acttcttaag tggtctcata aatttattga aggtaaaaag
    21061 atagaagatg ttttgtatat aaagtctaaa gaaaactata aagtaaatgt gattaagtct
    21121 ttagaaatta aggaattgga aatagtgaga aaacaacaga taccaccgta catgtagtat
    21181 taataatata taaaattaat atgtaatact cacaatagga tataaatggt atatatggta
    21241 ataatttata ttaaaaaaac ttttataaag tttatatttg ggtataaaag tattaattag
    21301 acattaatta ggaggaactt aaatatgata agagtatgtc caaactgttc aaatgtggat
    21361 gtaaataagc taaaaacttt ggttggagaa gaaaatgtaa agactggttg tataggtcag
    21421 tgtagagcat ttaaaaaaga agctgttgga ataatagaca atgagcttga aataaaggaa
    21481 acagaagcag agttttttga agcatgtaaa gaataaattt taaataaaat ataaattata
    21541 ataaatagag catctaaatt gtataagcat taaacaattt agatgctcta tttttatata
    21601 tacatttaat tatgagtata cttttctatt aaatacaaat aaatataact aatacattta
    21661 tttattaaaa aaatattgat ttaatatacc tatagggggt ataataaaaa tatagatttc
    21721 taaataagta atttatcaat attacatatc aaaataaaac gttacaaatt agctgaaaat
    21781 atattgaaag aaaggaagat tttattatga gttcaaataa agtggtagaa agttataaaa
    21841 ttacaggtat gacatgtgca gcttgtgcaa aggcagtaga aagagtaact aagaagatgg
    21901 atggagtata tgaccaaagt gtaaatatag ctactgaaaa acttaaaata gaatatgata
    21961 attctaaggt taattttgat gatataaagc aggttgtaga aaaagcagga tatggaatta
    22021 taaaggaaga aagtaataaa aaaattgata tgaaaattga tggaatgaca tgtgcagctt
    22081 gtgcaaaagc tgtagagaga gtggtaaaaa aattggatgg agtagaaagt attagtgtga
    22141 atattgcaac agataaagct aatatagact atgacccatc aaaagtaaaa ttatctcaaa
    22201 taaaagcagc tattgaaaaa gctggatata aaccaataga agaggtaaga aataaggttg
    22261 atgtggatga agataaattg agaaaagaaa gagaaatgaa gtctttattt gttaaattta
    22321 tagttgcaat agtatttgca gtacctctgt tttatatagc aatgggacct atgataataa
    22381 agcctatagg gccatggcct ttgccagaaa ttataaatcc tatgacaaat acttttaatt
    22441 atgcattaat tcagttaatt ttagtaatac cagtaatgat agctggttat aaattttata
    22501 taaatgggtt taaatcactt ttttctttaa gccctaatat ggattcttta gttgcaatag
    22561 gaacattagc cgcattttta tatagtctat atacgacact acaaatagcc aatggacaaa
    22621 ttcaaggtat gcaccatcat cagttgtatt atgaaagtgc aggaataata atagcactta
    22681 tcttacttgg taaatattta gaatcaaaat caaaaggaaa aacatcagaa gcaattaaaa
    22741 agcttatggg gctacaacct aaaactgcaa tagtgttagt tgatggtaaa gaagttgaaa
    22801 ctcctataga agaagtagag ataggagata tactattagt aaaaccagga actaaaatac
    22861 ccgtggatgg agttgtaata gaaggatata catcagttga tgaatcaatg cttacaggtg
    22921 aaagtatacc agtagaaaaa aatgttggaa gtaaggtgac aggagctagt attaataaaa
    22981 atggtgttat taagtttaag gctgaaaaaa ttggtggaga tactgctctt gcccaaataa
    23041 taaagttagt tgaagatgca caaggtacaa aagcacctat tgctaaactt gccgatactg
    23101 tatctggata ttttgtacca atagtaattg caatagctgt agttgcatca ttattgtggt
    23161 ttttaatagg aggaaaagat atagtatttg tacttactat atttatatca gtacttgtaa
    23221 ttgcttgtcc ttgtgcatta ggattagcaa caccaactgc tataatggtt gggactggaa
    23281 agggcgcaga aaatggaatc ttgataaaag gtggagaggc actagaatct gcacataaag
    23341 taaatacagt gatatttgat aagacaggta ctataacaga aggaaagcca aaagtaacag
    23401 atatagtatt aaataataat gtaaaagaag aatatcttat caagattgct tcaagtgcag
    23461 aaaaaggttc agagcatcct cttggagaag caatagttaa gtatggagaa gagaagaata
    23521 taaaatttga aaaagttgat aattttaaag ccataccagg tgctggtata caagttacta
    23581 ttaatgatga aagtatatta cttggaaata gaaagcttat gaatgacaat aatataaagc
    23641 ttggggattt agaagaaaaa tcaaatgtat tggcaagcca aggtaaaaca cctatgtata
    23701 tagctgtaga tggaaattta tcaggtataa tagcagttgc agatgtagtc aaagaaagta
    23761 gtaaaaaagc aatagaaata cttcatgata tgggaataaa agttgcaatg gtaactggag
    23821 ataatgcgaa aactgcaaat gctatagcaa atcaagtagg tatagatatg gttttggcag
    23881 aggttttacc agaagataag tcaaaagaag ttgaaaaact acaaaatcaa ggaaagtttg
    23941 ttgcaatggt aggggatgga ataaatgatg ccccagctct tgcaaaagca gatataggga
    24001 tagcaatagg aagtggaacg gatgtagcca ttgaatctgc ggatatagta cttatgaaga
    24061 gtgatttaat tgatgttcca acagcaatta aacttagtca tgaaactata aaaaatataa
    24121 aacaaaacct attttgggca tttggatata atacgattgg tatacctgta gcagcaggaa
    24181 tattatatgt atttggagga cctttattaa acccaatgat tgcagcagca gcaatgagct
    24241 taagttcagt ttcagtcgta tcaaatgcac ttagacttaa aaacttcaag gcatataaga
    24301 gagattagat taataaatta aataaaatgt atccaaaaga gctatcttta aattaaaagg
    24361 attaatttac aaattaagat agctcttttt tcatgtaaaa ttcacaacaa taagataaaa
    24421 tataagtaat atacgaaatt ttaaataaaa tagaaaggat tgatagaatg aattcgtcaa
    24481 tacttgtaat tgaagatgat tcaaatatac aagagttaat atcagaattt ttaagtgcag
    24541 aaggttatca agttgataca gcaaatgatg ggttggaagg tatacaaaag tttaagcaag
    24601 gaagttatga tttagtaata ttagatataa tgatgccaaa cttagatggt tatggagttt
    24661 gtaaaatgat aagaaagtca tcaagcgttc caattatatt tttaactgct ttaaatgatg
    24721 aaggagacca acttaaaggg tttgacttag aatgtgatga ttatataaca aaaccatttt
    24781 catttaatct tcttataaaa agagtagaag caattttaag gcgaagtaac aagactataa
    24841 atgacaaatt tatagttttt gaaaaattaa aattagattt aaacacatat attgcagaga
    24901 tagatggaga acctatagaa cttacactaa aagagtttaa tatattaaaa gctttgatag
    24961 aaaagtatcc acaagttata actagggaag gtcttttgga tagtatatgg ggttatgact
    25021 attatggaga tactaggatt gttgatgcac atataaaaaa tataagaaaa aaaatatcgt
    25081 taccatatat taaaactgta aaaggaatag gatatacact tgagaaggat atttgataag
    25141 tgggaaaagt taagtataaa gtataagtta ttttcaataa cgacatcatt attaatagcg
    25201 ttagctttaa taatatatct aattttatat tttttattgc catcatatta tcatgagtat
    25261 aaaattgagt ctcttcaaga aagtttaaaa tctttagtag atagttcaat tcatttcgat
    25321 acatatactt tagaagaaag actttattat atggctaaag accaaaattt ggctatttta
    25381 ttaaaagata accaagggaa aattgtatat ggtaagaatg aagtagttat tttaagatat
    25441 agtaagtaca tgattaatag tcttgaggat gagtatagaa cttctatacc tatatatact
    25501 aaagatgcaa aagatggtcc atatacttta gaactcgtta tgccattaca accaattgac
    25561 gaagcaaacg aagttataag aaaactaatg ccatacatca tatcaatagc aatattgata
    25621 gcgattattg gagcatatat atattcaatt gtaataacaa agcctcttat aaatataata
    25681 gagagtgaga gagaacagga atatagaagg aaggattttg ttgctacaat atcacatgaa
    25741 ctaaaaactc caataaccat aataagtggt caaattgaag gtatgattta tagtgtaggt
    25801 aagtataagg atagggatac ttatctgaaa aaatcatatg aatgtacaca agaattaaaa
    25861 gatttagtaa atgaaatgat agaggtttca aaatcagaaa tacttgaaaa agatttgaaa
    25921 ttggtatcaa taaatataag tgaattatta aatagacttg taaagagaca agtattttta
    25981 atagaagaaa aacatatgaa aactatttta aaaatagaag aaaatttgga aattaaagca
    26041 gaccaagaga gaataactaa ggcaatcaat aatataataa ataatgccat aaaatattct
    26101 cctgaaaaat cagagcttat aattagactc tatgataaaa ataaacgaat aagtaaaaag
    26161 acttctaatc aaagagtggt tttagaaatt gaaaatactg gtgtaactat cgagaaacgt
    26221 tatttagaag agatatttaa tccattttat agaatagaaa aatcccgttc aagaaaaact
    26281 ggaggaagtg gacttggact ttatatagtc agtcaaattt ttaaaagtca tgggtttgat
    26341 tatagcataa aaaacaaaga aaactctgta gtattcacag tagaatttaa aaattaatta
    26401 cttattataa agaatatata actttcaata atagttcatt ttaaaatcac aaaaaattca
    26461 taatgaaact gtattatata aatataaaga agcttaaaac cataaaatac accaactata
    26521 atgatgagat ttccttacat cctccatata agtcatcatt atagtaaccc aacaattaag
    26581 ttactatgaa tttaaaaaat aacaccgagc atataatcta tgcttagtgt tatttttatt
    26641 ttgaaataaa aaatatttaa tttattgaaa tgtatagatt tagacagttt aaactatatt
    26701 taagacttaa aaattataat tgatattaca aataaatata tgtactttgg gaaaaataaa
    26761 tataggaagt attatattta cagaggaggt agttttgatg gatagattaa aaagagatat
    26821 gaaaaatatg aaagaagatt taaaagaagg tgttaaaaag acagcttatg ttatgaaaaa
    26881 tacagctgaa gatttgaaag atgatgcaca aggtgctatg gtaaacttag aaatgaaaaa
    26941 agatgagatg atggaaagat atgaacaaaa gaaattttct aaagaattag agaagcaaat
    27001 gaagaatgaa atgaaataag cttttattta tgaataacaa attattgtat tcatataaaa
    27061 agtagccagt aatattaaat agtttattta aggaaataga ctttctaaaa ataactgtat
    27121 tagatttaga aagtctattt caaattagtt ttataaatag acataaggat aaagtagatg
    27181 ttatagaact agattttaaa aaatagatac taaggataa
//
LOCUS       DAMKPG010000047        27042 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMKPG010000047 DAMKPG010000000
VERSION     DAMKPG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.050"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(232..2511)
                     /locus_tag="PC621_002107"
     CDS             complement(232..2511)
                     /locus_tag="PC621_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4109262.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2782..4236)
                     /locus_tag="PC621_002108"
     CDS             complement(2782..4236)
                     /locus_tag="PC621_002108"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF4109263.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4400..5269
                     /locus_tag="PC621_002109"
     CDS             4400..5269
                     /locus_tag="PC621_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4109264.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5483..7555)
                     /locus_tag="PC621_002110"
     CDS             complement(5483..7555)
                     /locus_tag="PC621_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4109265.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
     gene            complement(7858..8700)
                     /locus_tag="PC621_002111"
     CDS             complement(7858..8700)
                     /locus_tag="PC621_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4109266.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8947..9441)
                     /locus_tag="PC621_002112"
     CDS             complement(8947..9441)
                     /locus_tag="PC621_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF4109267.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(9593..10564)
                     /locus_tag="PC621_002113"
     CDS             complement(9593..10564)
                     /locus_tag="PC621_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF4109268.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(10565..11542)
                     /locus_tag="PC621_002114"
     CDS             complement(10565..11542)
                     /locus_tag="PC621_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109269.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            11761..13320
                     /locus_tag="PC621_002115"
     CDS             11761..13320
                     /locus_tag="PC621_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4109270.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            13426..14385
                     /locus_tag="PC621_002116"
     CDS             13426..14385
                     /locus_tag="PC621_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109271.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            14388..15200
                     /locus_tag="PC621_002117"
     CDS             14388..15200
                     /locus_tag="PC621_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109272.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(15415..16317)
                     /locus_tag="PC621_002118"
     CDS             complement(15415..16317)
                     /locus_tag="PC621_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4109273.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            16672..17850
                     /locus_tag="PC621_002119"
     CDS             16672..17850
                     /locus_tag="PC621_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF4109274.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            17859..18515
                     /locus_tag="PC621_002120"
     CDS             17859..18515
                     /locus_tag="PC621_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF4109275.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            18515..19180
                     /locus_tag="PC621_002121"
     CDS             18515..19180
                     /locus_tag="PC621_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF4109276.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            19285..20061
                     /locus_tag="PC621_002122"
     CDS             19285..20061
                     /locus_tag="PC621_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF4109277.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            20123..21457
                     /locus_tag="PC621_002123"
     CDS             20123..21457
                     /locus_tag="PC621_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF4109278.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            21954..22880
                     /locus_tag="PC621_002124"
     CDS             21954..22880
                     /locus_tag="PC621_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF4109279.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(23272..26811)
                     /gene="nifJ"
                     /locus_tag="PC621_002125"
     CDS             complement(23272..26811)
                     /gene="nifJ"
                     /locus_tag="PC621_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF4109280.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         9348 a         4293 c         3553 g         9848 t
ORIGIN      
        1 tactattatt actatctaaa gtattttgct ataatcccat atactcttac ctcaaaaaca
       61 ttcaaatgga aaaaatacta catattaata aactttgccc aataattcct aaaataatat
      121 cctaacaatg atttatgttt ttataatagt atatttttac ataaaaaagt gaacattgat
      181 taacaaagtt cactttttta taaattttta tgttataatt cacaatttta tttaatttgt
      241 ttcttttcta tttaaactat atctatctaa tattaatttt tcatattctt ctattggcat
      301 tggtctagca aataaaaatc cttgggccat atcacaacct ataaacctta aaaactctgc
      361 ttgctctaca gtttcaacac cttctgaaac aacttttata tcaagttgct ttgccatttg
      421 aacaatactt gaaactacaa tttgactctt ttttgcattt cccactgtta taaagaattg
      481 cctatctaat ttcaatacat caattggcat atctttaagc atatttaaag aagaataacc
      541 agaaccaaaa tcatccatag aaattttaaa tccaacagat ttcaaagttt gcattatttt
      601 aaataatatt tttgtattgt caaatattgc actttctgtc aattcaatct caattaaatt
      661 tggttcaatt tcatattctt ttgttattct ttcaagctca gaaataaaac ctggattctc
      721 aagatgtact cttgataagt ttattgatat aggaaatgtg gggaaatttt cattttccca
      781 acgtttaaaa tttttacaaa cttgggcaaa tacaaacata tccaattctg ttataaaacc
      841 atttttctca aaaataggaa taaatacacc tggacttatt agaccttttt tgggatgttg
      901 ccaacgtaca agcgcttcag cacctacaat tttagtagtt gataaatcaa ccttaggctg
      961 taaataaact ttaaattgtt cattttttaa agcatcatgc atttcttgtt caatatcacg
     1021 ttcttctatg agagtttctt ttaaattttc agcataaatc tcataaactt ttaaatattt
     1081 tctttttact gtagtttttg caatcatagc tttgtcaaga caggtagaaa tatttgtttc
     1141 tccctcttca acaaaatata cacctatgga tggaattaaa ttgagttcta ctcccaaact
     1201 tgtttttatc attgacaatt ttttacgtat aagttcacat atttctataa ttgactctct
     1261 attatttttc ttttcaaata ttatagcaaa attgtcattt gaaattcttg aaaatacaga
     1321 ttgctcttta aatatattct taattatctc tgctatttcc tttaatactc tatctccttc
     1381 ttcataccca aacctatcat ttactagctt aaacttatct atatcaaaat aaattaaaac
     1441 tagattcttt ttctcatgag aggataaaaa ttttcctcct tcaagattaa atttatttga
     1501 attgccaata cctgtaacgt tatctttaaa agcaaattct tccaattttt ccttactctt
     1561 tttatgtata aatagtatat aaattgttaa tgccgtaaag attataatca acactacaca
     1621 tgctgataat gtaagtttta taatagcgta tgatttctca gatatagcag ttttaggaac
     1681 ttcagaaaaa agataccaat cattgattcc tattggggca taacttaaat aatatccttt
     1741 tccaaacata ctataactta atgaacctga tttaaagttt tttatatttg acttcatatt
     1801 attgatacta gattttgata ttgaatcact tctgccaatg taagaaaata aattttcttt
     1861 agtagagttg gcgtttttgc tgcttggatt taaaacaata ttaccattac ttctagctat
     1921 ataagaatat ccttttccat taaatgtagg tacagaaagt attttttcat acaacttcgt
     1981 actatgtgtg gcaaatataa ctcctttaac ctcattatct ttataaattg gcacagaata
     2041 tacattaata ttactattgg tatttttatc atcttctatt acatctttta atttttctgt
     2101 tataaccact tctccttgca ttgattttaa gaaataatct cttttagaga agtcttctat
     2161 ataaccatct gtagtgtatg ctgtaccatt tggtaaaata actcccattc ttttaaatga
     2221 actattaata gcttgtcttt ttagtataga aaggatatta tctacttcaa aagactcttc
     2281 tccctcaata aatattgaaa ttgactctag taactttata tctccattaa tttgtttttt
     2341 taacacattg acgctttgat ttgctatttc tgaaagatat tgattcgttt cttcacctaa
     2401 tgtcttattc aaccttgaac cataaatcaa acaggaaatt gtaattacta agatacttat
     2461 gacagcaatt atggacaaac catacctatt attttttgca tcacttaaca ttatgcttcc
     2521 tgcctttctc gaatctatgt atgtataatc attatcttat gacaattatt gtaaacattt
     2581 tataataata cttatttttc catatttact gtaattatac aaaattataa actttattac
     2641 aacaataaat taattaatat agatattagg gtttatatta ttgcattatc tattttacaa
     2701 tcatttacat ataaaaaaat gtaatatcaa agtgaaactt gatattacat ttttgttctt
     2761 gtattttgtt taatttttat gctacatttt acttaaacac tcatttgcaa cttctttatc
     2821 atcaaaatga tgcttaactt tacctataat ttgataagtt tcatgtccct tacctgttat
     2881 aattacaaca tcatcctttt ttgccatact tatagcttct gctatagctt gttctctatc
     2941 aacaacaaca tggtaatttt cttttttctt gtcaattcct tcaagtatat cttttattat
     3001 tgcctctggt tcttcagttc ttggattatc agaagttata atacataaat cagaatattt
     3061 ttgtccaatt gctcccatca aaggtctctt ttcagtatct ctatctccac cacatccaaa
     3121 cacagaaatt atttttcctt ctgcaaattg ttgagttgtc tttagtacat tttcaagtgc
     3181 atcaggagta tgagcatagt ctacaattac acttatacct ttatcatttg gaactgtttc
     3241 aaaccttcct gatactccat tagaaagtct aagaccttct ttatatattg gtttaggtat
     3301 gccaagtaca tagcaagcag ctataactgc taaagtatta tatactgtaa acattcctgg
     3361 aactggtata aatattactt cttcatatga aggtgttatt aatctataag atactccatc
     3421 cgaatctgat tttatatctc tagccataaa atcagcctta gtatcaacac cataagtgta
     3481 acaaggtaca tttatacctt ttatattttc atatatcttt tttccacctt catcatctat
     3541 gtttattata tttgccatag tagtcttaaa aaataatttt tcttttgctt ttctataatc
     3601 ctctaaatct ttatgaaaat ctaaatggtc tggtgttaaa tttgtaaata tgcctagttt
     3661 aaagtctgtt tcatcaactc tatttaatgc caatgaatgt gatgaaactt ccatcgcaca
     3721 gtagtcacat ccattatcca acatattatt aaaatgatat tgtaaatcta tactttctgg
     3781 agttgttgaa ttggaaacaa tgtctttttc tccgtcaaaa atttttatag ttcctataag
     3841 tccaacttta ttcttattta aagttaatat ctcatttagt atagtagtta tactagtttt
     3901 tccattagta cctgtaactc ctataacgtt aaatttctga gatggatgat tataaaaatt
     3961 atcagctact tttgccatat cttttcttgt atcttttacc tttataaatg tacatccttt
     4021 tatttctaca tcttcttcaa caagaaatgc acttgctccc ttttctatgg catcttttat
     4081 atacttatga ccatctgaca caaatccttt tatacatata aataatgtac cttttgtaac
     4141 ctttcttgaa ttatattgta cattattaat atctatattt aattcaccct tgacattgat
     4201 tatatccaac ccctgtataa tatcatttaa tttcataaat gctttcctcc ctttaaactc
     4261 tttctgctaa aattacacta catataatat tatattaaga attttttact atgtgaatgg
     4321 ttaaaaatgc ttgaaatatt gcaaaaaatg ctctataatt ttctataata taactgagga
     4381 aaggtcttct ataaaaacta tggataataa aatcgggtac ttaaacgata actttaaaat
     4441 atttaatatt agagataaaa aagatataca atttgaatac cataatcatg attttaataa
     4501 gataattata tttataaatg gaaatgtaac ttattttgtt gatggtattc cctataaact
     4561 aaagccttgg gatatactct ttatcagcaa taatgaaatc cataaaccag ttatagattc
     4621 aaatgtcttt tatgagagaa ttgcaatatg gattagccca gaatttatga aaaaaaatag
     4681 tgatgctact agtgacttag aaaaatgttt taaaatagct acggagaata aatgcaatag
     4741 attgcgttta aatatacatg atattgaatc tattaagagt tttattgagc aaataaatat
     4801 ttccgaatcc aatactgaat ttggaaatga aatattgaga aatgcattat ttatacaact
     4861 tatgatattc ttaaacagaa ttttcctaaa aaatgaaaat atggaatcaa cagaagacgt
     4921 tttttatgat gaaactatta agaaagtatt aaactatata aattcaaata tagatgataa
     4981 gctttcaata gatagcatat cgtctgaatt ttttataagt aagtattatc ttatgagaaa
     5041 atttaaatct catactggaa cgtctataca caattatata gttcaaaaaa gattaatttt
     5101 agcaaagtca ctaataacca atggttacct tatgagtgaa gtttgtaata aatgtggatt
     5161 caacgattat tctagttttg taagggcatt taaaaaaaac tacggtgtat ctcctaaaaa
     5221 ttatataaac aataaaaatc ctttagatat tactctttta gatgaataga taatttatgt
     5281 aaattttatt ttaataaaaa cttgaagtat tagtgtattt atcttactaa tacctcaagt
     5341 tttttaatgt tttaaaatat atcaaaatat aatgatatat atttctatta cttaaaatat
     5401 atattaaata aattgcatgt taactgaacc tatttatata tttattaaat ctcacataac
     5461 ttttagttat actatcatat agctatccta ttttttcaat atcatatctt aatatactta
     5521 ctctgttctc ctcttttgct gtagttattc cttttttaat gcttaatttc tgattttgtt
     5581 ctaaattcgt aaatactaca ggacttattg ttgatagaca atttttttca atatattcta
     5641 agtccatttt aactaatttt tctcctgctt ttacttcatc tcctacattc acaaatactt
     5701 caaatccatt tccttctaaa tttacagttt ctatccctaa atgtataagt atttctactc
     5761 cacttttttt ggattttatt attattgcat gcttagttgg aaatataact ccaatagttc
     5821 catcaactgg tgaataaatc aatccatcac tagatttaat tgcaaatcca tcccccatca
     5881 gtttacttga aaatacagaa tctggcactt cttctatatc taatatctct ccatttgtta
     5941 atgaatcaat cttttctact aaagcatttt ccatattggc aacttccata ttttttttca
     6001 ctttattttt atcaacattt ttagtaacag tactttcaac ctttgttttt ccctctatta
     6061 tatcttttat atcagacttt attgaatccg attttggtcc aaatatagct tgaacattgt
     6121 tgccaataac cataacacct gctgctccaa gtaatttcaa tttttcttta tctactttat
     6181 ttatatcatt tacaaccaat ctaagtcttg ttatacacgc atctaggctc tttaagttgt
     6241 ctttatttcc taaagcaatt aaaatctctg atgctaattc attcttagac atttcaatga
     6301 cttcagcgtt agtttctgac tcatcttctc ttcctggtgt ctttaaatta aattttctta
     6361 tcataaatct aaatacacta tagtataata ctgcaaaacc aatacctacc aagatagcca
     6421 agtaccatgg agttctatta ggaagaacac caaataataa gaaatctatt aatcctcctg
     6481 aaaatgtgaa tccaatttta actcctaata attgcataat tgcgtatgat aaacctgaaa
     6541 ataaacaatg tattgcaaat aatactggtg caacaaataa aaacgcaaac tctattggct
     6601 cggttattcc tgttaaaaaa gaggttaatg ctgctgatgc cattatacct gctacaaatt
     6661 ttttcttttc tggctttgct tcatgcacca tagcaagagc tgctgctgga agaccaaaca
     6721 tcataaatgg aaatgcacct gtcataaaag tacctgctgt gaaatttact ccatctttaa
     6781 gttgagcaaa gaatatcttt tggtcaccca ttacaagctc accagcttta tttacatatt
     6841 ctccaaattg ataccaaaat ggattatgga aaacatgatg caaaccaaat ggtattaata
     6901 gtctttccaa agaaccttcc ataaatgctg ctagtgtttg atttgcttct ataacatttt
     6961 ttgagaaact aagtaatccc tcttgtattg gaggccaaat aaaagacaat ataattccta
     7021 ttgctatagc aactaatgaa gtaacgattg gtacaaatct tttacctgca aaaaaaccta
     7081 gatactgagg taattcaatt ttataaaact ttttataaag ataagcagcc actatcccta
     7141 taataatacc accaaatacc cctgtttgaa ttgttggtat tccaacaata cttgcataca
     7201 tagaattgtc taccatatct tctgtaacac ccgccataac acccatagat gtattgagaa
     7261 ttaaaaaacc tactatggct gccaatgctg caacaccttc tccttcagtt aaaccaacag
     7321 ctaccccaac tgcaaatata agtggtaagt tattaaatat aatttgacct gaactttcca
     7381 ttatacttat tataagttga aatgtaggat tcgccaaagc tggaaaatga gacaacaatg
     7441 cgtctcctgc taaagcatta ctcacgccaa gtaatatacc tgctgcagga agtaatgcaa
     7501 ctggtagcat tagagatttt cctacttttt gtaaaacacc aaatactttt ttcatttact
     7561 tttctctcct tattatttta tattttttta aataaaaaag acttaagttt ataaaaagtt
     7621 acattagtat acttaattct aaaattaaac ttatgttaat ataaataaac ctatattaac
     7681 ctaatttcag aattaaaaat aaactattat aattttttta taaacataag tcttgcctgc
     7741 attatcagta acaatcttaa tgttttaaaa ttattatttt atattaaatt ttaaataata
     7801 tattaaaatt aaacataata tattaaacct atacattctg tattaaacta ctctttatta
     7861 taaattcagt acagatataa ctcttttgat atgtaatgtt aaataaagta gttcatcttg
     7921 acttacatag taatcataat tcttttctac atgagttttt attttatatg cacaatcata
     7981 atccttttca tactttactt taattatatc tataatttca ttatttgtat cattgatttt
     8041 ttcttttttt actaatcgtt ttgcaaagaa ttttaaatga gtcaaaagcc tatcataatt
     8101 tatatcatct tctttaaatt caactctata ataatatctt ataatattta gtatgtcttt
     8161 tacaatttta gtaattaaca gtgattcctt tgaacttccc ttatagtttg aatttactat
     8221 atgcattgct ataaaaccag cttcatctac tggaaatttc acatcaaatt ctttatttat
     8281 ataatctaca gcccatttac caatctcata ttctttttta tgtattcttt ttatctcatg
     8341 tagtaaatca ttctttatag ttatattttc atgatatctt ttaattgcaa atgcaatatg
     8401 gtcagtaagt gctacataaa tatattcata aagcttagta ttcaaattct cctctgcata
     8461 agcaattatt tcagtactaa tctcaaaaaa tttctcatca acctggcttg ctagtttgct
     8521 aattttatct aaaaagcttt catcttgtat tacaaaagtt ctttcaattt tacttttatc
     8581 tatttcttga cctttctttt tttgaaaccc aatcccacat cctgttagta ttaattcttt
     8641 ttttatattt tcatctaaaa ctaatacaac attattatta taaatttgtt gaataatcat
     8701 aatcaatatc tctcccaaaa tacaatttcc cttattcata aaaaaatacc taaattaaga
     8761 tacaattcac ctgtatacct taacctaggt attgcctgct taccagtaac aatccagctt
     8821 gttaattctt atatattgta ttcaatttat ggttttattc tattataaat aaatatatat
     8881 gtcaagtaaa ttattgaaaa ctatttccta ttttttagta ttatttttaa catcaaaaat
     8941 caaattttat tccattatat ttatgattct atcatcatca tttaataaag attcaccttc
     9001 aagtatactc atcatatttt taggaaaatt taatccttgt gtgattgatt ttactgctac
     9061 taaatcagtt gaagatatta ctgctgccaa agcaagtgaa acagataatg gcatcctagg
     9121 aataagaaaa tgtataaaga ttccacctgc aaacacggtt atcaaaacta aaccaaatgc
     9181 aagaagcaaa attggttttc taagctctag taactctttt cttgatacat tttgtccttc
     9241 actgaataat aatggagcta tgatacatat tacaaatatt tctggattaa atgaaaatga
     9301 taatttaaac ggcaaaatag acaatataat ccctagtaca atttgatata ttgcaagagg
     9361 aatattactc cactttttgc taaatacagt tgaaatcttt gtacaaacca ataatcccaa
     9421 caatgttact aaagttgaca taactacctc tcttttcaat aataatatta taaattaaac
     9481 ttatatatca actaacttaa tattaacaac atgaaatccc atcttaatca aaaaaatttt
     9541 cagtttattc tattattctt attatataca tcaaatttaa tttttgaaat atctatacta
     9601 gatggcatgc aacaaaatga tttcttgata tttcttttaa aactggaatc tcttttttgc
     9661 atatttcctt acaataaggg catcttgtat gaaacttaca tccacttggt ggatttgttg
     9721 gagatggtat gtctcctttt aatataattc tatttttctt aattgtaggg tcttgtactg
     9781 gtactgcgct cataagtgct cttgtatatg gatgttgaga atgtaagtaa agttctcctt
     9841 tagttgcaat ttctattata gaacctagat acataacaca aactctatgt gcaatatgat
     9901 ttactacact caaatcatga gatataaata aatatgagag tttatattta tcctgtaaat
     9961 ccataagtaa atttataatt tgagattgaa tagaaacatc tagtgcagaa acaggttcat
    10021 cacaaactat aaattctgga tttaatgcta atgctcttgc tatacctatc ctctgtcttt
    10081 gacctccaga aaactcatct ggttttctat ttaaatggta cttagaaaga ccacaacttt
    10141 ctacaacatt taatatatta tcttttattt cattctttgg cactaaatta tgttctataa
    10201 ctgcctctga tattatttgt tccacattta atcttggatt taatgaacta tatgggtctt
    10261 gaaatatcat ttgcattttg ggtcttaact ttctaagttc ttccttctct aacttatata
    10321 tatctacacc attaaattca actttacctt cagttttttc aattaatcta aggatggttc
    10381 ttcctgcagt tgattttcca catcccgatt ctccaactat agccaaagtt tctccttttt
    10441 ttaaatcaaa agaaattcca tctactgcct ttacacattt aatttcctta tcaaataatc
    10501 cagatttaac ttcaaagtac tttttaagac cttctacttt taataaactc tcttcttttt
    10561 tcaattatat ctcctccttt ttatataacc aacatgcaat actatgacca ctattcaatt
    10621 ctattaaagg aggtatctga gttctacatt tatcacatac tttttcacat ctatcactaa
    10681 aataacatga atctggtatt ccaattggat ttggtacttg tcctggaatc gaatacaatc
    10741 ttttatcttt gttttttcca agaacaggct ttgattttag aagtccaata gtataaggat
    10801 gttttggatt tttaaataat tcaacaacct ctgccacttc tataatttta ccagcataca
    10861 tcactaatac tttgtcacac atttctgcaa ctactcccaa atcatgtgtg atcatcatta
    10921 tagatgtgtt taatttttct tttatatttt tcattatatc taaaatctga gcttgaatag
    10981 taacatctaa agctgttgtt ggttcatcag ctataagtaa ttttggatta catgataaag
    11041 ccatagctat cattattcgt tgtctcatac cacctgataa ttcatgtgga tatgaattat
    11101 atatttcttc agctcttggt atttctacca gttttatcat atcgattacc tttttcttag
    11161 cttcttcctt tgataaatct tgatgtatca taataccttc aatgatttga cttccaacag
    11221 tgaacgctgg gtttaaagat gtcattggtt cttgaaaaat cattgatatc tcattccctc
    11281 ttattaccat aagttcatct tcattcatct ttaaaatgtc tttgcctcga aagattattt
    11341 ctccaccttc tatttttgat gttggaagta acttcatcaa agacatggct gttacagact
    11401 taccacatcc tgattctcca actattccaa tagtttcacc ttcatttata ttaaaactta
    11461 cttcatttac actctttaca atccctttat ctgtatgaaa ataaactttt aaatctttaa
    11521 cctcaattag tgctttactc attattttct cctctcagca aaattactta aaataacata
    11581 caccttttga ttgtatttta taaaaattta aattacaaca ttttcttaca ttttattatt
    11641 tttttctatt aattataata atataatagt taatttctat atatattgat ttaataatga
    11701 ttactattat acaatgaaaa atatgtgtta aatattattt tatggaggtt ttataagaat
    11761 atgaaattta aaaaattggc atcattaatc ttagtttcat cattaatgct tacatttaca
    11821 gcctgtgctt ccaatgataa agacaagtct agtggaagca aatctggtgg aactgttgta
    11881 atacctatgg caagtgaccc agatataatc aatggagctt ttgcaaacgt taaagaagac
    11941 tctcttgcat caaatatagt ttatgctcct ctttatactt atgaaaaagg gaatttagta
    12001 aattatttag cagaaaaagt agattttaaa gatagtaagg aattaactat aaaattaaaa
    12061 tccaatttaa aatggcatga tggaaaacca ataactgctg aagatgtctt atttacattt
    12121 aatacagttt tagacgaaaa acaaaattca ccatcaagac aatacttatt ggtgggtgaa
    12181 aaaccagtta aagtagaaaa aattgatgat ttaacagtta agattacttt accaactgca
    12241 agtgaatctt tcttatatgg tatatctaaa atttctccaa taccaaaaca tgtatttgaa
    12301 ggagaatcaa atatagctaa gtcagaaaaa aataataatc cagttggttc tggtgcattc
    12361 aagtttaagg aatggaaaaa aggtgaaagc atagtatttg aaaagaatgc agattatttt
    12421 ggtggagaac ctaaggcaga ttctatagcg ctaaaaataa ttcctaatga agcatctcaa
    12481 gaagcagctt taaataatgg cgaaatttca cttatgaaga catctgctga aggatatgaa
    12541 aaggcaaaat caaactcaaa cttacaaact tatacttata gtgaagaaag attaaactac
    12601 attgtattta accaaaatat atcaaacatg gctaacaaag aagtaagaca agccttatca
    12661 tatgccttaa atagaaatga aatgatagaa tcagcatatg gaaaagaagg ttctgtacct
    12721 gctaaatcta tattagttcc agaagcagac ttctatacag aagaaggtgt tgaaggatat
    12781 gaccaagata caaataaggc taaagactta ttggataaaa gtggtgttaa aattgataaa
    12841 ttaaaaatag gttataacac aggtagattt gggcataaaa actatgcctt agtagctcaa
    12901 caagagttga aaaaaattgg tatagaagct gaaatagttc cttatgaaag taaggcattc
    12961 tttaatatac tatttagtaa tagcactgaa tgtgatatgt atgtaaatgg ttatgcttgg
    13021 ggattagaac caaacccata tagaggtatg tttgaaactg gtcaatattg caaccaaact
    13081 aaatattcta atgctgaaat agatgcttta tgggaaaaag gatttactga gttaaacaag
    13141 gaaaaacgta aagaaatata caaacaaata caacaggata tatctaagga tgcacctatt
    13201 tacactatag attatgaaca aaatttaatg gcagctcaaa aaaatctaaa aggaataaaa
    13261 gatgctaaac catctcctgc aatattattt gaagattggt ctaaactgta tgtagaataa
    13321 ttgatgtaaa attttatatt attaattaga aaaggagatg tctaaaaata atattgtttt
    13381 attattttaa aacgagacat ctcttttaat taggagaatt tacttatgtt aaaattaata
    13441 ttgaatagag taaaagtttt aatacctatg ttaatcttaa tatctatatt atcatttgga
    13501 cttttagaat tagctcctgg tgacccagca gatgcatata taaatccttt aatgtcttca
    13561 caggatattg aaaatataag ggttaatatg gggcttgata aaccagtata tataagatat
    13621 ttaaactggt tgaaaaacac tttaagtgga aacttaggta tttcttatat aaaccatatg
    13681 cccgttacag aacaaattat ggaaaaaatg ggaaatacat ttattcttat gggtacttct
    13741 ttgatatttt caatattggt agctatacct cttggaatat ttttagctgt aaataaaaat
    13801 tcaataacta gtaaagtatc atctgttttt aattatatag gtgtttctat cccttctttt
    13861 tggataggta tgatactcat ttcaattttt tcagttaaac ttaacatttt cccatcagga
    13921 ggtatgcata ctattggaaa tgatagtatt ggagatttag taaaacattt agttttgcct
    13981 gtaataactt taggtctata taatactgct atttttacaa attatgtaga agctagtgta
    14041 aatgaacaat taaaaaaaca atatgttatt acagcaagag caaaaggatt atcagaaaaa
    14101 gtcatactat ttaaacatgt cttgaaaaat tcactgacat cattggttac aatcctaggc
    14161 atgagtatac aaaaattagt cactggtgca tttgttacag aagtagtttt ctcttggcca
    14221 ggtatgggaa gattaatgat tgattcaatt ttttcaagag attatactgt aattatggca
    14281 ataactatgt tatcagcact atttttaatt ttaggtaatt tagttgctga tatactatac
    14341 ttattaattg accctaaaat aaaatctagc aaaggaggat tctagttgtg tttacaagta
    14401 tcttaaaaaa attaaaaaaa gataaatcct ttgcttttgc atgtattact cttagcataa
    14461 taatcatatt aattatagct tcttttatat taaatataga ctctaatgca attgatacta
    14521 cttctaaact tcaagaacct agcttaaagc atatctttgg tacagacgaa cttggtagag
    14581 attacttctc tagagctctt tatggtggaa ggatttcact tagtgttggt attttatcaa
    14641 tgcttatctc tgtagtattt ggtactgctg taggtgttat tagtggatat attggaggta
    14701 agctagacca attcttgatg agattagtag atatgcttat gagtatacca agtttcttag
    14761 ttttgattgt attaaatact tatctaacac ctaaagtttc cactattata ctaattattg
    14821 gattcttaag ttggatggaa gtttcaagaa ttgtacgtgg cgaaacatta aaaataaagg
    14881 aaaatgaata ctgtttagct gctaaagcta ctggtatcaa gactagaaat atactattaa
    14941 gacatatagt tttaaactta aaagagacta ttgtagtcgc tggttcgtta aatattgcta
    15001 atgctatctt aactgaatcc gctcttagtt ttttaggtct tggaatccaa ttacctcttg
    15061 catcatgggg taatatgctt caagatgctc aaagacatat ttttgacaag ttatatttag
    15121 ctgtatttcc aggattactt atatttttaa cagtcataag tttgactata ataagcaaaa
    15181 aattttctag taatacttaa caaattttat atagaaaata aaaacagtga ttatctaaag
    15241 atatacactg tttttatttt tcgtttaaaa aatacatatt ttgtatatta taactttaaa
    15301 tactactcta ccatgttatg taaagcctat tattgctctc aaacatatag atgaaaaact
    15361 aatagtttat tgtaataaaa attgatttat ttatttgtta ttttaataac atttttaaga
    15421 gttcttttta ctaaagaaat ctataataaa tttaaataca tgttggtcta catggctata
    15481 acgttctttt ttaggtctac atagtataac tttccatgta ataggatgat taaaaggctt
    15541 ttctataatt ttgctatttt caaaaaaatc atgaactggt gatggtaaaa ttgttataaa
    15601 atcagaattt gtggttgata gcaacaaaaa atcccatgaa ccagacatta tatatctttt
    15661 taacttaaca ttttcatcag taaatttttg cattagctta tgatgtatca taaaagtagg
    15721 attaaatatt gcaagtaatt gctcattcaa atcgttccaa tctatcttat ccttcttagc
    15781 aagtggatga tttatattta aaaatgctgt aagttcatct tcttgaagca catgttcagt
    15841 tactacactt ttatcaattt tatgtggatg taacagtact gcataatcta gttcctgcaa
    15901 gataagcatt ctatttaagt catatgcacc cttctctact atatcaaatt ctatatctgg
    15961 attatttgca actaactgtg caactacatc tgaaaatgct attccaagta taagtggagg
    16021 tattcctatt ctaacttttc ctttaaattg aactgaatct tctcttaaat cttccatcat
    16081 atttttgtat tcatttataa tatgttctgc atttacataa aatctttctc ctgctggtgt
    16141 taacccattt aacctacctt tatacctttc aaataaatat acatcttcac tttcttcaaa
    16201 attttttata atttgactta atgctggttg agatatgtga agtgtcttag atgctattga
    16261 taaattaaaa ccagatttca ctatttcaac aaaatatttc aactgtttta tatccatttt
    16321 aaaccccctt ccccaaaagt ataatttaga ttatattttt tcttctaatg atattttata
    16381 ggaaaatcaa cataatttca ttaatatttt aacaaaatat ttttctaaat ctactataaa
    16441 tttttatcct ataaattttt cttatacact atataaaaaa ttagacttag acaaaatgct
    16501 tttttcgccg taatctataa ttagaaacag gaaaacctaa agcattagga ataaaagtaa
    16561 ttgaaatact taaaaacaag cttgtattaa aaaagtactt tattatagtg ttccctaatt
    16621 tctttattca gaaaattttt aattatcatt caagaaaggt aactattaga tatgaaagat
    16681 gtagtaatcg tatcagcagt cagaacacct ataggaagtt ttggaggtgt atttaaaaat
    16741 acttcagctg ttcaacttgg aactatcgct gttaaagaag cgatatcacg tgtaggactt
    16801 aatttatctg aaatagatga agtaataatt ggaaatgttc ttcaaacagg gcttggacag
    16861 aatgttgcta ggcaaattgc tataaattca ggtataccaa attctgttcc aagctacaca
    16921 gtaaataaat tatgtggttc agggcttaaa agtgttcagc tagctgccca atcaatcaca
    16981 tctggtgaaa atgatgtggt aattgcaggt ggtactgaaa atatgagcca agctccttat
    17041 atcgttccaa ctgctcgctt tggtagtaaa atgggtgaca taaccatggt agatagtatg
    17101 ctaacagatg gccttattga tgctttcaat cagtatcata tgggaataac tgcagaaaat
    17161 attgcaacta aatttgaatt tactagagaa atgcaagata aattggcatt agaaagtcaa
    17221 aataaggctg agaatgctat aaaaaacaac cgctttaaag aagaaatcgt tccagtagat
    17281 gttttaatac gtcgaggaaa aatagaaact attgataaag atgaatatcc taaattaggc
    17341 atgacatttg aaggtttagc taaacttaaa cctgcattca aaaaagatgg tactgtaaca
    17401 gctggaaatg cctcaggtat taatgatggt gcggctatgt taatactaat gagccaacaa
    17461 aaagcagacg aactaggtat tagaccactt gctaaaataa aatcctatgc ttcagctggt
    17521 gttgaacctg aagtcatggg tactggtcca atccctgcaa ctagaaaagc actgaaaaaa
    17581 gctggactta gcatcaatga tatagactta atagaagcaa acgaagcgtt tgctgcccaa
    17641 gcattagctg ttaaaaatga acttcaaata gattcatcaa agttaaatgt taatggtggt
    17701 gcaatagcat taggacatcc aataggagca agtggagcta gaatacttgt taccttaatt
    17761 tatgaaatgc aaaaacgtaa agttgaaact ggacttgcaa cactttgtat tggtggtggt
    17821 caaggaatat caatggtggt ttcacgatag gaggaaatat gaataaaata gttagcattg
    17881 atgaagctct atctcatgta aaagatggag atacaataat ggttggtgga ttcatggcca
    17941 atggttcacc agaaaattta attgattatt tatgtagtaa aaatataaaa gatttgacac
    18001 taatttgcaa tgatactggc tttattgaca aaggagttgg caaaatggtt gtaaataagc
    18061 aatttaaaaa gataattgct tctcatgtag gattaaataa agaaactgga agacaaatga
    18121 acaacaatga aacagatgtt gagttagttc ctcagggaac tcttgctgaa caaatacgtg
    18181 cagctggtta tggtctagga ggtatactca caccgactgg tattggtact cttgttgagg
    18241 agggaaagca aaaaattgtt gtcgatagca aagaatacct tcttgaaaaa ccaatttatg
    18301 cagatgttgc tcttctattt gcatcaaagg tcgataaagc aggtaatcta gtttataaag
    18361 gctctatgaa caattttaat aatttaatgg catctgctgc caaaattaca atagttgaag
    18421 cagatgaaat cgttgaaatt ggtgacattg acccaaatga agttaataca ccaggtattt
    18481 ttgtgaatta catcgttgaa ggaggtaatt tataatggat aaattagaaa tgcaagaata
    18541 tattgcaaat agagtatcta aagaattaaa agacggagcc gttgtaaacc ttggaatagg
    18601 tctaccaacc aaagtagcaa attatattcc ggataatgta aacgttatct tacaatctga
    18661 aaatggcttt ttaggtttag gttcagccga agatggaaat tcaagtgatg aaactattgt
    18721 aaatgcaggt ggtcaaccag ttactatact tcctggagga tgtttctttg atagtgctac
    18781 ttcttttgga ataatcagag gaggtcatgt tgatataact attcttggcg cacttcaggt
    18841 tgacaaatat ggcaatatag caaactatat gattccaggt aagatggttc caggtatggg
    18901 tggagctatg gacctagtaa ctggagctaa aaaagttatt gtagcaatgg aacatacttc
    18961 aaaaggttct gcaaaaatat taaataattg tactcttcca cttactgcaa caaatgctgt
    19021 agatttgata gttactgaaa tgggagttat ggaagtaaca tcagatggaa ttttacttaa
    19081 agaaataaat ccagctttca cattagatga tgttatttct gctactgaag ctcctttaat
    19141 attgtcagat tcattaaatg gaaaaatatc tgttgtttaa aataattaaa actagaaaat
    19201 aaaaatctct tagacgtatt acatataaat ttgtattgct taaaatatta atataaaaat
    19261 aaaataaaat tggaggaaat gaaaatggtt aaagataaag ttgtattcgt aacaggagct
    19321 gcaagtggaa taggtaaaca aatagctgaa tcgttcttaa aaaatggtgc taaagtaatg
    19381 ttctctgata taaatcaaga agctttaaat aataccataa cagagttaca aaaaaaaggt
    19441 tatgattgca tgagtgtaaa gtgtgatgta actaaggaag aagaaattaa ccacgctata
    19501 gataaaactg ttgaaaagta tggcagatta gatatattaa taaacaatgc tggacttcaa
    19561 catgtttcta tgatagaaga tttcccaact gataaatttg aatttatgat aaaaataatg
    19621 ctgacagcac cttttatagc tactaaaaaa gcatttccta taatgaaaaa acaaggcttt
    19681 ggtcgtataa taaatatggc ctcaatcaat ggtgtaatag gatttgctgg aaaggctgct
    19741 tataactcag caaaacatgg acttattgga ctaacaaaag ttgctgctct tgaagctgcc
    19801 gattctggta taacagttaa tgctttatgc cctggatatg tagatactcc acttgtcaga
    19861 ggtcagttta atgacctttc aaaaactcgt aatataccat tagaaaatgt cttagaggaa
    19921 gttttgtacc cacttgtacc acaaaaacgc ctattagatg ttcaagaaat tgccgactat
    19981 gctatgttcc tagcaagtga taatgctaag ggtattacag gacaagcttg cttactagat
    20041 ggaggatata ctgcacaata gttattatat ttagggcttg aagaaagtat tcttcaggtc
    20101 cttctactaa ggagggatat ttatgagtat tatcgcacca attggtataa ttattggtat
    20161 catagctatt atatattttt cagttaaaga aattcatata actatagctg ctccaatagc
    20221 aactttaata gttgttctct taaataaaat ggatgttgta acatcaatgc taggtgctga
    20281 caaaaacaat tatatgggtg cacttggaaa ctatataatg agttattttg caatattctt
    20341 attaggctca atacttgcta aatttatgga agaaagtgga gccactgtat caatagctga
    20401 ttttatttta aataaagttg gctctaatca tccttataga gttttagttg ctatatttat
    20461 agtaagtttt atattaacgt atggtggtat aagcttattt gtagttatgt ttgcagtcat
    20521 tccacttgca agaactctat ttaaaaaact tgatatttct tggaacctta ttcagatacc
    20581 actttggctt ggtattgcaa caattactat gactataatt cctggaacac cagccattca
    20641 aaatgttata cctattcaat atctaaatac gtctcttact gcagctacta taccaagtat
    20701 tgtaggtagt ataggttgtg ctacgtttgg acttgtctat atgaaatatg ctctaaacaa
    20761 aagtattaga aaaggtgaaa cttacgcaac ttacacttca aaattagaag aagaatcttt
    20821 agatagagaa ttaccaggat tttttgcaag tatttttcct ctattagcac tcgtaattat
    20881 agctataact ggaagtactt ttggttcaga atttatgaaa aaaaatgtaa tttacatagc
    20941 tttaatagtt gcaattcttt tatctgcttt tttattccat aaatttctat catctaagat
    21001 acagacattg agcataggtg ctagtggttc tataggtcct atatttgcta catcttctgc
    21061 agttgcattt ggagcagtaa taatggctgc acctggtttt gatgtattta gcaaacttat
    21121 aatgtcaata ccaggtagtc cacttataag tcttactgtt ttgacttcaa caatgtcagc
    21181 aataacaggg tcctcttctg gtgcattagg aatagtaatg ccaaactttg cagattatta
    21241 tttaagtact ggtctaaatc cagaattaat tcaccgtgtt gctacaatag cctcaaatat
    21301 actaactata gttccacaaa gtggtgtttt tcttacattc ttaagcttat ctaagttaac
    21361 acataaaact ggatttaaag aaactttcat tgtagttgca gttggttcac taatagcaga
    21421 aattattgta attacattag gattactaat gtattaatat aaaacaaatt aaaattcata
    21481 atatgaaagg tggtgtctca aagtgaactt tttacttaat gagacactct ttttgtatga
    21541 aatcaaatat attttaatca tttcaacaat aaaaaagaac ctttctctat tttgagacaa
    21601 acccttttta ttccttaaag atttaaacaa ttataatttg ttactaatac gaaaacatct
    21661 attgaataaa tttaaccaaa taaaaaaaaa tttatcataa ctataaattt acgttatgga
    21721 attattataa aaatattatt ttctctaagt aagtaattcc atactataca ggcttttaat
    21781 acaaattttc aaacacactg aaatatacct atttattcca tatatatctt ttttacatat
    21841 ctttacagta aaaaaagtct taatcttatg ttagtataga ttcacgggta ccccctacac
    21901 taaaggttac aaatcagtaa catttttaga atttttagat taagaggtgt ttaatgatga
    21961 gaagaaatat accaacaaaa ttaaaaacta tattagcttt aggagtgtta actttagcaa
    22021 cagcttcaag tgctgttact gcaagtgctt catcacttga aaataatgta aatataagta
    22081 acaaaagttt agtatatagt aatactaaat acaagttttc tgcaaaacca aattgttctg
    22141 taaaaccaga acttaaacct gataaagata actcttgtaa ggataagaat cataatgaca
    22201 agaattgcaa caacactaat aaacctgagg acaacaataa ttcagactct actaataagc
    22261 ctgataacaa taaaccagag tctaataaac ctgaggacaa caataattca ggctctacta
    22321 ataaacctga taacaataaa ccagagtcta ataaacctga agacaacaat aactcaggct
    22381 ctactgataa acctaataac aataaaccag agtctaataa acctgaagac aacaataatt
    22441 cagactctac taacaaacct gatgataata acaactcagg ctctacaagt gaaaattttt
    22501 cagcttacca aaaagaagtt gtcgacttag taaatataga aagagctaaa gctggattaa
    22561 atcctcttac tcttgattct tctatatcaa atgttgctac aaaaaaatct caagatatga
    22621 ttgataacaa ctatttttca cacaattctc caacttatgg ttctccattt gacatgttga
    22681 aaaaatttgg aataagctat aagacagcag gtgaaaatat agctatgggt caaaaaactc
    22741 ctaaagaagt tgtaaacgca tggatgaatt ctgaaggtca cagaaaaaat ataatgaatc
    22801 caaatttcag taaaataggt gttggagtag cacaaaaaag tggtggttca atttattgga
    22861 cacaaatctt tgtaggataa aatactcttt ttccatgcat atcaaaagag tatctctaag
    22921 tgataaaaaa cacaaatgag atactctttt gtatctattt taaaattatc aaatccaaat
    22981 atacatacta taaatattat ttgttctctt aattttagtg tgtagaacaa tatataaaaa
    23041 gtattggcaa gatgaaaatg gaaaacttaa aaagatattt aataggtaaa aacaggatct
    23101 taatatcttc ataaaaaaag gctgtctcaa aatagagata aggccttttt cattgttgaa
    23161 atgattaaaa tatatttgat ttcatacaaa aagagtgcct catgaactaa aaagctcact
    23221 ttgagacact ctcttttatc aattctatat aagcattata atactttaat attattcagc
    23281 taattttttg tatccttcat atctttcttt agcatcagct tcagctttag caaataatcc
    23341 atcagcttcc tctgggaatt gtttagctat agcagaatat cttacttgtg caagtatgaa
    23401 atctttgaag cttgcagttg gttcttttga atctaaagtg aatgggtttt taccttcagc
    23461 ttttacttct ggattgaatc tgtataaatg ccagtatccg caagcaacag cttgtgcttc
    23521 attagccaca gttcttccca taccttcttt taatccatgg tttatacatg gagcataagc
    23581 tattattaga gatggcccat catgtttttc agcttctaaa acggctttga taaattgatt
    23641 tttatcagcc cccatagcaa cttgagctac atatacgttt ccatatgtca tagccatcat
    23701 tcctaaatct ttctttctag attttttacc tgaagcagca aacttagcca tagcagcagc
    23761 tggagtagct ttagaagctt gacctcctgt atttgaatat acttctgtat caaatacaag
    23821 tacgtttaca tcttcaccag atgcaagaac atggtctaat ccaccgtaac caatatcata
    23881 tgcccaaccg tctccaccta gtatccattg agatctctta actagataat cttttctatc
    23941 ttttatagct tttactaatt ctttagcttt ttcatctgta caatcacaag ctggtttttc
    24001 taataattca acaaccttag cacttgctac tttagaagct tctccatcat ttctagaatc
    24061 taaccactct gtaaatactg ctttagcatc ttcacagata tccatagaaa ctaactctgt
    24121 catagcatct actattttat ttcttatttg cttaacagct gtgtacatac caaatccata
    24181 ttcagcatta tcctcgaata aagaatttgc ccaagatgga cctttacctt catggttaca
    24241 agtataagga attgatggtg cagaagctcc ccatatagaa gaacatccag tagcattagc
    24301 tatcatcatt ctatctccat ataattgtgt aactacttta atgtacgcag tttctccaca
    24361 tccagcacat gctcctgaaa actccattaa tggttgtcta aattgacttc ctttaacagt
    24421 atttgcaggc ataacatcaa cttttggagc tacttcatca ggatttactg caaatgccca
    24481 attatctaac tctgtatcaa gttgagtatc tattggcttc ataactaaag ctttttcttt
    24541 agctggacat atgtcagcac agtttccaca tcctgtacag tcaagtggac taacttgaat
    24601 tctatatttt aatccttcta atcctttacc taatgctttt ttagcttcaa atccttctgg
    24661 agcattagct aattcttctt cagtaactaa tactggtctt atacatgcat gaggacatat
    24721 aaaagaacat tggttacatt gaatacagtt atctattatc cattcaggaa catcaacagc
    24781 tataccacgt ttttcataag ctgcagtacc acaagggaat gttccatctt ctataccatt
    24841 gaatgtactt actggtaagt tgtttccttc ttgtgcagtc attggtctta gtatattttt
    24901 tataaattct ggttctttaa cttctccttt atctgcatca acagcatttt cccaagaagc
    24961 tggaacttca actttaacta aagcatttat tcctgcatca acagcagcat agttcatatt
    25021 aactattttt tcaccttttt taccataagc tttaactata gaatctttta agtatttaac
    25081 agcatcttct tctggtatta tttcagctaa tttaaagaat gcagattgca ttatcatgtt
    25141 tattctgttt ccaagaccta tttcttgtcc tagtttaaca gcattaactg tatagaattt
    25201 tatatcattt ttagctatat attgtttcat atgagctggt aaatgttctt caagtcctgc
    25261 ttggtcccat atagtgttaa gtacaaatgt accacctttt ttaagacctt ttaataaatc
    25321 atattgataa acatatgatt gtttatgaca ggcaatataa tcagcttcat ctattaaata
    25381 agtagctctt attggagtat caccaaatct taagtgagac atagttatac caccagattt
    25441 tttagagtca taatcaaagt aagcttgagc atatttttca gtatggtcac cgattatttt
    25501 gatagcttgt ttattagctc caacagtacc gtcagaacct aatccccaga acttacatct
    25561 tatagttcct ttagaagcta tttttattgg ctcagatgga gttaaagaag tattagttac
    25621 atcatcaact atacctactg taaatccatt tttaggttgt tctgaaacta agttatcaaa
    25681 aacagttttt atatctgaag gagtagtatc ttttgaacct aatccgtatc ttccacctat
    25741 tatcatagga gcattttctt ttccatagaa tacatcacgt acatctaagt ataatggttc
    25801 accagttgaa cctggctctt tagttctatc aagtacacat attctttcaa cagtagatgg
    25861 catagcatct aagaaatgtt tcattgagaa tggtctatat aaatgaactt taactaaacc
    25921 atatttttct cccttagcat ttaagtaatc tatagtttct tctattgttt ctgttactga
    25981 acccatagct attaatacgt atttagcatc ttctgctcca tagtaatcaa ataaactatg
    26041 ttttctacct gttaaattac tcattttttc catgtatttt tcaactatac ctactatatt
    26101 ttgatagtat ttattagaag cttctctagt ttggaaatat atatctgggt tttgagcagt
    26161 accacgagca actggatgat ttggagataa agcattatct ctaaatgctt gaactgcttc
    26221 aaagtttaat aaacttgcat aatcttcatt ttctagtaat tcaacttttt ggatttcatg
    26281 tgaagttctg aatccatcaa agaaatgtat aaatggtaat ctaccttcta ttgcagctaa
    26341 atgtgcaaca ggtgctatat cagctacttc ttgaactgaa ccagaagcta ataatacaca
    26401 tccagtttgt ctagcagcca taacgtcttg gtgatcccca aatattgata acgcttgaga
    26461 tgccaaagca cgagcactta catggaatac tcctggtaat aactctcctg caactttata
    26521 catgttaggt atcattaata ataaaccttg agatgctgta aaagtagaag ttaaagctcc
    26581 tccttgtaaa gaaccgtgga atgttcctga tgcacctgct tctgactgca tttcaacaac
    26641 attaactgtt tgtccaaata tattttttct tccttgtgat gcccactcat caactacttc
    26701 agccatagtt gaagatggtg tgattggata tatagctgct acatctgtaa acgcataggc
    26761 aacatgagct gcagctgtat ttccatcaag tgttttcata aacttagcca taatatgtat
    26821 cctccttgat agctacaatt gtacttgttt ataacaagtc tattttttta tatttacata
    26881 taatttacac aacaaaaata taaacacaca tatagtcgcg caaatactct atcttctaat
    26941 ataatactac agattagata aaattccttt gaaaattgta aattaattac atatttaagt
    27001 agattttttt taaaattaaa tatgttaagt ttttttcata at
//
LOCUS       DAMKPG010000048        26946 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMKPG010000048 DAMKPG010000000
VERSION     DAMKPG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.051"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            134..1993
                     /locus_tag="PC621_002126"
     CDS             134..1993
                     /locus_tag="PC621_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4109281.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2285..2884)
                     /locus_tag="PC621_002127"
     CDS             complement(2285..2884)
                     /locus_tag="PC621_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4109282.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2987..3784)
                     /locus_tag="PC621_002128"
     CDS             complement(2987..3784)
                     /locus_tag="PC621_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF4109283.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3897..4002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4094..5080)
                     /locus_tag="PC621_002129"
     CDS             complement(4094..5080)
                     /locus_tag="PC621_002129"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF4109284.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5564..7294
                     /locus_tag="PC621_002130"
     CDS             5564..7294
                     /locus_tag="PC621_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF4109285.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7404..7484
                     /locus_tag="PC621_002131"
     tRNA            7404..7484
                     /locus_tag="PC621_002131"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7438..7440,aa:Leu,seq:tag)
     gene            7716..7889
                     /locus_tag="PC621_002132"
                     /pseudo
     CDS             7716..7889
                     /locus_tag="PC621_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     regulatory      8381..8485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8695..11169
                     /gene="gcvPA"
                     /locus_tag="PC621_002133"
     CDS             8695..11169
                     /gene="gcvPA"
                     /locus_tag="PC621_002133"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF4109286.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11169..12626
                     /gene="gcvPB"
                     /locus_tag="PC621_002134"
     CDS             11169..12626
                     /gene="gcvPB"
                     /locus_tag="PC621_002134"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF4109287.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13049..15406
                     /locus_tag="PC621_002135"
     CDS             13049..15406
                     /locus_tag="PC621_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF4109288.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15492..15809)
                     /locus_tag="PC621_002136"
     CDS             complement(15492..15809)
                     /locus_tag="PC621_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF4109289.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            16147..16902
                     /locus_tag="PC621_002137"
     CDS             16147..16902
                     /locus_tag="PC621_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF4109290.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
     gene            complement(17541..18812)
                     /locus_tag="PC621_002138"
     CDS             complement(17541..18812)
                     /locus_tag="PC621_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF4109291.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   19459..20674
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19459..19487
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            21083..21370
                     /locus_tag="PC621_002139"
     CDS             21083..21370
                     /locus_tag="PC621_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109292.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFITIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(21908..22459)
                     /locus_tag="PC621_002140"
     CDS             complement(21908..22459)
                     /locus_tag="PC621_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109293.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            22650..23612
                     /locus_tag="PC621_002141"
     CDS             22650..23612
                     /locus_tag="PC621_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF4109294.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            23907..25751
                     /locus_tag="PC621_002142"
     CDS             23907..25751
                     /locus_tag="PC621_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF4109295.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASNAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            26073..26657
                     /locus_tag="PC621_002143"
     CDS             26073..26657
                     /locus_tag="PC621_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF4109296.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT        10218 a         2995 c         4220 g         9513 t
ORIGIN      
        1 aactatataa aacaaggttt atatattcta atagtataga aaattttatt gtaaacgcat
       61 gtatattatt agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg
      121 ataaaggtgt tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt
      181 gatttttata agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt
      241 gattaattat gttgggattg tttgtggagc tagccaacgt ctaactaaac tagagatgaa
      301 tcatcaacca aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac
      361 agggcatgga gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact
      421 tttattagaa aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga
      481 acagaataac cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt
      541 gtttaaaata gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat
      601 tatatctatt ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat
      661 ggttagactg gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac
      721 aggagtaaat actatagaaa aatttaaatt ggatgcaaat caaaatattt gtataaataa
      781 ggataagaaa tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat
      841 atttggatat gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga
      901 catagggaaa tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg
      961 ctatgtagat aaggaagatt tagtagtaag acaacgagat gtagacaatg aactaattaa
     1021 tataacaaat gaaattaaaa ataaacatag tattactgtt gtcagtggaa tttgttgcat
     1081 tgaagatgta aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca
     1141 aaaaactgtt aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa
     1201 aaaaatgatt gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga
     1261 atttatagtt tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga
     1321 agctttagtt cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc
     1381 agtacttgag aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg
     1441 taaatggatt agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt
     1501 ttcaagaata caattttata acactaaatt tgtagaaact tattccaata tacaaaataa
     1561 atacaggata cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa
     1621 tcaaaaacat cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt
     1681 agatgatttt ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt
     1741 tttaaagctt gactctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt
     1801 aataaaaaat attgtacata tgttaaaaga gcttaatatc acaactgttg ctgagggcat
     1861 tgaatgcaag gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt
     1921 tgtattttat aaaccaatgc caatattaga atttgaagaa atattggata aagagtttgt
     1981 ttataatagc taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac
     2041 tgaataaatt tttactttat ttacagaata gtgattattt acagaaaagg gcgaaaagtg
     2101 tattccttta agtatgggat ggatagcaca aataggggag aaaaaatatt ggccatataa
     2161 gaatgcataa taatataaat atttgaaagg tcagcattat ataaaaatta aaaagaaaat
     2221 aaataatttt tctataaaaa agagactatg agaaaagtta ctcatagtct cctaaataat
     2281 gtatttattt tcttcctgca acccatttta agccccaatg aaattcagta tccatttctc
     2341 tatgaccttt gaagtattta actacttttt caacactgaa tgtctcatca tttacatttt
     2401 ctatcaatgc tattgaacac atattatagc catttttatg ttcatcaagt ttgacaacta
     2461 agtctgaatc ttgtttttgt ttgattgtta caattccatc agcttcagac cagttagcta
     2521 caccttcgta tataaaagca taaataagta ttctttttat atcttttatt ttattaccat
     2581 ttattcttaa attttctcct tgtgtattac ttcctgttct gtcatctcca tctaattgag
     2641 caaaaggtgg attgtctaaa ctaccaaaag catttcctaa tgcttgaaca gcacctttta
     2701 caccattttt catctcaaat aggcaaccca aatctaaatc tatattactg cttcttaaag
     2761 agccaaaaaa accctttttt tgtacttttt gattccaatt taaattaact aagatttcac
     2821 ctaaatcgct attttccttt tttgtaaggt ttattttttg tccctttttt aattctatag
     2881 ccatagtaat cttcctccac aaacatagat ttatttaaat ggtagcactt aataagtcaa
     2941 tattcaacaa gattagaata ataatattaa aactatacta aatatactaa aaagtaggaa
     3001 tcacaatacc tttgtatttt tcttcaatta agcttcttgc tttatctgat gtaagaacat
     3061 cttgtagaac tttaatcttt tcactgtctt tatcttcagt tctaaatgct aaaagatttg
     3121 catactcgtc atttatagga gctgtataaa ttccgttttt cttggcatca agaccagcag
     3181 gtacagcata agtagtatct ataaatgcag cagtaacatc tggaagagaa gatggaattt
     3241 gagcaatatc taactctttg aattgtattc cctttggatt ttctattatg tccttaggac
     3301 taactaatcc atccccttta ggtaacgtta aaagtccaat actttcaagg tatctaaggt
     3361 tcttagagca agaagatggg ttattgctta ttgcaattgt atctcctttt ttaaattcat
     3421 caacagattt tatcttataa gagtaaagta ttgctgggca tacatatagt tttttaccag
     3481 ccattatttt gtaacctttg gccttaacag cttcttttag ataaggttca tgttgaaata
     3541 gatttgcatc aatttcacca ttatttaaag cttcgtttgg aagtatgtaa tcatcaaagt
     3601 ttttaacttc taaagtatat cctttttctt taataagtgg ttttaattct tctaataact
     3661 ctcctcctgg aactaaagta gcaccaacaa caattttttt atcatcttta gctttagaac
     3721 atccaaccac agctaaagtt agtactagac ataataacaa acataataat tttttgaatt
     3781 tcatataaat tccctcctga atataattga ttagttaaaa ataaaatacc attctttatc
     3841 acactgcaaa ataaaaataa gcctgagttt aaactcaggc tttaaaagct aatttagact
     3901 catctttcag tataatactg caggatttag caccaatgta tataaaaata tactggttgc
     3961 tgggtttcat agggccagtc ccttcaccac tctggataag aagtatcaat ttttaattat
     4021 gttataaata ttatcatgat tttttacgtc tgtaaacata attttataaa tattttaaaa
     4081 tctttaatgt attttattta acacctaaaa ttccagaaac tagaatatca gtatcagcac
     4141 ctaagaaata gtcatttatt tcaatagaat ccagagtatt tctcaaagca ttttcttcat
     4201 gccttatacc aataagttta ttttctacaa atgcaacatc acattttcca aaaaaatctc
     4261 caaaaaattt tatactagtt attatgcctt tatcaacatt taaattaaac tcaacatttc
     4321 caccagaata ttttaattca tttgaaagag tatattttgg tgaattacca aaattccaat
     4381 cccaagtttc gtattttgat ttagtaagct tttctatttg ttttatatca tgctcattca
     4441 aacaataatt agtattatca gtatttgttt tagaaagata atccattata gcatctttaa
     4501 attctaatat gtccattttt gaatgtaaat gttcacttat attggttaca cgagatttta
     4561 cagattttat tccttttcct tcaaatttta taggtttaac ctttaatgct tttgagaggt
     4621 catttatttc agatgaaaat aaaagagtac catgatgcat tactttgttt ttgtagttgt
     4681 attgagcatt tccagaaaat ttcttgccat ctataagaag gtcatttctt ccagaaaatt
     4741 cggcattgac atctagtgtt ttaagtaaat ctattatggg ctgagtgaat cttctgaaat
     4801 cactaaaatt attattgtta caagctataa atgtaaaatt tatatttccc aaatcatgaa
     4861 atacagctcc accaccagac tgtctcctaa cgacaggtat agaatattct tttatatatt
     4921 ctaaatttat ttcagaaagt gtgttttggt tttttccaac aattatagaa ggttcattcc
     4981 tccataaaat aaataagtct tcatcaaaat tttttagaag atattcttcc attgctaaat
     5041 taaaataagg gtttgttttt tcattataaa ttaataacat taaagtagtt cctccataga
     5101 attgataaaa tttttattat gaatacaatt aattttataa ttcgacaata atataaaaac
     5161 tcctttaaat tagaaatttt aaaaactacc ttgcaaagtt taaataaata atatatactc
     5221 aaaatatgtt taataaaagt gaatagaagg gtagaggcgc aataatctat cagtactaat
     5281 aaggagttaa gcacagagag ttattaggaa aggagatatt gccgaagttt tataatcgat
     5341 gctttaagat tatattgctg ggggtacata gaaaatatgt acaactgtca caggtttatt
     5401 tgtggtgagc tatcatttaa ggaattatat tatatttatt tttactattg ttgatatatt
     5461 gtaatgttta agccttgagt gtttaccact caaggctttt ttagttttat tgtaacataa
     5521 taccctgtac agttcacaaa attaaaagga gagggagatt tatatgagga acaaaaaaat
     5581 taatattatt tttctgacga caatcatgtt cataatgtca acagtaatgg tttttgcaga
     5641 agaagatata gacactattg cactagctaa tgcagaaaaa tttgggattt taactttaat
     5701 accaccatta gttgctataa tacttgcatt tataactaaa aacgtaataa tttctttact
     5761 tataggtatt ctttctggta gttttataat taaagctagt ggtattaatg tttttgcgac
     5821 atttatacag gcatttttag atttagtaga tagagcttta gtatctttag cagacccttg
     5881 gaatgcaggg ataatactac aggttttggc aattggagga gtaataaatc ttgttgctaa
     5941 aatgggagga gctaaagcta tagctgaagc acttgctaaa agagcaaagt cagcaaaagg
     6001 gacacagctt ataacatggt ttttggggct tttagtattc tttgatgatt atgccaattc
     6061 actaatagta ggtcctatga tgagacctgt tgcagataaa atgaaaatat caagagaaaa
     6121 gctagcattt ataatagatg ccacagcagc accagttgct ggtttagcta taatatctac
     6181 atggataggt cttgaggttg gtttaataca tgatgctttt gaaagtataa gtatagatgt
     6241 agatgcattt ggtatatttt taaatacaat accatttaga ttttacaata tattaatatt
     6301 agctttcatt gtaatatcag cattattatt aaaagaattt ggtccaatga gaaaagctga
     6361 gataaaatct agaagtagaa aaattagtat agaccttgat gaaggcgtag aagagttgga
     6421 tgatttagct ccaaaaaatg gagtaaaatt gagtgtatgg aatgctataa tcccaatagg
     6481 aacattgata atagttgcat tagcatcatt ttattatagt ggatatacat ctataatggg
     6541 aggagacgat aaggctctga tacaactatt tacaaattct ccatattctt ttgaagcaat
     6601 aaaagaagca tttagtgcat ctgatgcatc tagagcatta tttcagtctg cacttgttgc
     6661 aagtttagtt gctattataa tggctgtagt aaaaaagatt tttacaatat cagaagcaat
     6721 tgatgtttgg atagatggaa tgaaaagttt agtaatcact ggtgtaatat taatacttgc
     6781 atggtcatta agttcagtaa taaaagagtt aggtacagct aagtttttaa tacacctatt
     6841 atcaggttca ctaccaccat tcttattacc aagtttaata tttggtttag gagcaataat
     6901 atcttttgct actggaacag cttatggaac aatgggtata cttatgccac ttgcaattcc
     6961 attagcatac tcattaaatc ctgatatgtc ttatgtaata gtaagtacta gtgcagtttt
     7021 aacaggtgca atatttggag accattgttc tccaatttca gatacaacaa tactttcttc
     7081 aatgggagct ggctgtaatc atatagacca tgttaacact caaatgccat atgctatatt
     7141 tacagcagta atcactattg tatttgggta tataccagct ggacttggat tgccaatata
     7201 tatagtatta ccagtggcaa tagctgctat atttgtaggt attcaaataa ttggtaagaa
     7261 agttgatgaa gcggaaatag aacttgtaga ataaagatat aaataagttt caccttttat
     7321 tatcactatt ttgttagttt ttagataatt ttaaaaagtt attgaaaata atggcgaaat
     7381 atagtaaaat aaaagagtga taagcggatg tggcggaatt ggcagacgca ctagacttag
     7441 gatctagcgc cattggcgtg ggggttcgac tcccttcatc cgcactttac agtacttaat
     7501 tgtactgaaa aaaactgggg ttgctggctt tcggagtggg ctatcccgtt tttatttttt
     7561 tgtatatata atagattggc actttagtat aaggtgcttt ttttataggc aaaaacagta
     7621 catttatttc tgttttatta tcattcatct attagaatat actttattta agaatgtgtt
     7681 aataagcagt gttcacattc ttgataaaat gtggaataat atgcctacca ataagcattt
     7741 ggtgtataag taagagtatt tatatagttg attgtgaaat gagtacaaca attctgtaca
     7801 gtataattgg cattataatt gtagcattga atttaaagtt tgcacagtcg ctgattggaa
     7861 aatttacaaa gtggatgtca aacatataaa ttttagttta ttggacagtt caaggaatca
     7921 gaaattggaa ttttatagta ttaatatttt atagtattaa tataatgtat aaaattttat
     7981 agcaatgtat ttcataagta taaagtgact agaattttaa gaaatataca aattctataa
     8041 aaactaaata atttcaagta tattttaaaa atcttatttt tcacaagttt aaaattgtaa
     8101 acgatttcat caagtctatg aatatacttt taacatgtta tagtaaatct caatataaat
     8161 ttacatttgg ttctttgtgt gttattttaa taataaatta agtgaaaatg ttattttaca
     8221 ttaatatata atatctttgc ataagagtga ataatataaa agagaaagaa ttgataataa
     8281 aaacttaaca aaaaaattaa tacataaaaa aaaataaata taatgtgttg acatttaatt
     8341 gaaatattca tacaataaaa ttgaaaataa atatttccaa atgagattag cgagagagtc
     8401 ctttatacga aaaggcgccg aagaagaaat ttaagaaatg ccaattttta gagaagcttt
     8461 caggcaaaaa tatcgctaat tgatgggact ctggagagac ttaagttaag atactataag
     8521 tcaccgaaga agcaaaataa tattaaatta ttaaatcttt caggtataca aacagggata
     8581 tataggctag gattattttc catgctgata tatctttttt ttatgaaaaa gtacaggaaa
     8641 taatatcaaa gttacttaaa tacagtaggt ctaattttct agggagggtt atatatgaat
     8701 gaattgaaaa gagtatcttt gtacaatata cacaaagagc ttggagcaaa acttgttgaa
     8761 tttgctggct gggaaatgcc tttagaatat gagggtataa ataaagagca tgagaaggtt
     8821 agaaaaagtg caggtatatt tgatgtatct catatgggag aggtacagat aaagggagca
     8881 gagtcagaaa aatttataca aaacctagtt acaaatgata taagcacatt aaagataaat
     8941 gacattatat atacacctat gtgttatgaa aatggtggag tagttgatga tttattaata
     9001 tataaatttg gtgaagaaga ttatttatta gtaataaatg caggtaatat agataaagat
     9061 gtagcatgga taataaaaca aagtgaagga tacaatgtag atataaaaaa tatatctagt
     9121 gaagtgagtc aattagcaat tcaaggacct aaagcagaag aaattcttca aaaaatcaca
     9181 gatatagact taaactcaat aaaattttat aagtcaattc catcaacaaa agtatgtggt
     9241 tgtccttgtt tagtttcaag aacaggatat actggggaag atgggtttga gatatactgt
     9301 aaaaataaat atgtagaaat aatttggaat gaagttctaa aggttggagg agaagatata
     9361 tgtccagctg gtctaggttg tagggatact ttgagatttg aagcagctct acctctatat
     9421 ggtcatgaaa taaatgaaca catatctcca attgaaggtg gtttatctat atttgtgaaa
     9481 acaaataaag aatcatttat tggtaaatca attttatcaa aagaaaaaga aagtggagct
     9541 aaaagaaagt tagtaggatt tgaaatgcaa ggtaaaggaa tgcctagaaa tggatatgat
     9601 ataaggattg gtgataaaac agttggattt gtaacaacag gatgtgcatc tccaacgaca
     9661 ggaaaaattt taggtatggg aattatagat tctgaatatg caaaagtagg aaatgaaata
     9721 ggtatagcaa tcagaaaaaa agttgttcct gctgtgatag ttaagaaacc tttttataaa
     9781 aaacaatata aaaaagataa tattatatta aacaaagaaa ataaattttc atatatacct
     9841 gctacaagtg aagataaatc aaaaatgtta aaagtagttg gattaaattc ggttgatgaa
     9901 ttattttcag atataccaga agaagtaaaa ttaaaaagag atttaaattt ggaaattgga
     9961 aaatctgaat tagaggtaag caaaatagta aaaagattgt cagaagaaaa cttaagttta
    10021 gaagacttga cttgttttct tggtgctggt gcatatgacc attatatacc atcaattata
    10081 aaacatataa cttcaagatc tgaattttat acagcataca ctccatatca agctgaaata
    10141 agtcagggaa ctttacaagt agtttttgaa tttcaatcta tgattgcaga aattacaggt
    10201 atggaaattg caaatgcatc aatgtatgat ggagcaactg ctgcaataga agcatgtata
    10261 atggcaatga atcagactag aaaaagtaaa atagtagtgt caaaaactat tcatcatgaa
    10321 actttgtcag tattaagaac ttacttacaa tataaagatt gtgagatagt agaaattgac
    10381 ttttgtaatg aatatgggac tacagatata gaaaaattaa aagcttctgt agacaaggat
    10441 acagcatgtg ttcttataca aactcctaat ttctttggaa ttattgaaga aatggaagaa
    10501 atagaaaaga taactcatga aaataaggct atgttaatta tgagtgtaga cccaatatca
    10561 ttaggagttt taaaaacacc aggtgagata ggtgcagata ttgttgttgg agaagctcaa
    10621 tcacttggaa accctcttaa ttttggaggt ccttatgtag gatttttagc tagcaaatct
    10681 aaatatacta gaaaaatgcc aggaagaata gttgggcaaa gtttagatgt agaaggtaaa
    10741 attgcatatg tattaacttt acaaactaga gaacagcatg taagaagaga aaaagcaact
    10801 tctaatatat gttcaaatca agctttaaat gctcttgtag cttcaatata tatggctact
    10861 atgggtaaag aaggatttaa agaggttggt atgcagtcta tgaaaaaagc acattatact
    10921 tacaataagc ttgtacaaac tggaaaatat aagccaatat ttaaaggtaa gtttttcaaa
    10981 gaatttgctg tacaaggaaa tcttaatata gaaactataa atgataagct cttagaagaa
    11041 aatatattag gtggttataa tttagaatac aattatccag aattaaaaaa ttcaactttg
    11101 ctttgtgtga ctgagaaaag aagcaaagag gaaatagata agttagttgg aataatggag
    11161 ggattataat gaaagaatac aatagtttgt tgatagatat atctaaaaaa ggtaggaaag
    11221 catactcact tccaaagtta gatatagatg atataaaaat agaagatatg attgaccaag
    11281 atatggcaag acaaagtgag ttgaatttgc cagaagttgg tgaattagaa ttagttagac
    11341 attatacatt attatcaaat aaaaactttg gagttgatac aggtttttat cctttgggtt
    11401 cttgtactat gaaatataat cctaaaataa atgaagatat ggcagcactt ccaaacttta
    11461 ctggaatgca tccataccaa tcttcggata cagctcaagg ttctctttct ttgatgtatg
    11521 atttatcaag aagacttgca gaaattacag gtatggatga agttacacta cagccttcag
    11581 caggttctca tggtgagttt acagggctta tgattataaa agcatatcat gaaaatagag
    11641 gtgaccataa gagaactaaa gtgataattc cagattctgc acatggaaca aatccagcaa
    11701 gtgcagctat ggcaaacttt gatgtgattc aaatagcatc tgataaaaat ggagctgtag
    11761 acataaatgc attaaaagag gttttgaatg atgaagtagc agctcttatg cttactaatc
    11821 caagtactct tggtttattt gaaaaaaata taaaagaaat agctactctt gtacatgaag
    11881 caggaggact tttgtactat gatggtgcaa acatgaatgc tattatgggg ataacaagac
    11941 ctggagatat ggggtttgat gtggttcatc ttaatcttca taagactttt tcaactcctc
    12001 atggaggtgg aggtccagga gctggtcctg ttggagttaa aaaagaatta gtaccttttt
    12061 taccaatacc attaattgaa agaaaagaag ataagtatgt actaaattat gatagagaaa
    12121 aatctattgg gaaaattaaa aatttctatg gtaattttgg agttcttgta agagcttaca
    12181 catatatact aactatggga agggatggac ttaaagaggc tagtgaaatg gcagttttaa
    12241 atgctaatta tataaaggaa agtataaaag atgattatat tttgccaata gataccttgt
    12301 gtaagcatga gtttgtatta ggtggattgc caagaggaga ggccaatata aaaactatcg
    12361 atattgcaaa aagactacta gattatggat accatcctcc aacaatgtac tttcctctta
    12421 ttataaatga ggcacttatg atagaaccta cagaaagtga gagtatagag acattagata
    12481 gttttataga agctatgaaa agtgttgcta aagaggcaaa ggaagaacca gaactattga
    12541 aaactgctcc acataatact ttggtcaaaa gagtagatga tgcaagggca gtaaagaaac
    12601 ctatactaac atggtcacaa agataaataa ttaaaaagta gagatttaaa gtataaagag
    12661 aattaaattt gtaagaaaaa gctatctaac ttaatccagt ttagatagct tttttgtata
    12721 ttagtatgta tggtattttc tatataaagt tttaaattat attagtgctt atatagaata
    12781 ttgatatgat gattatgtat agatattaat aagaattgtt gaatttatct tgccttaact
    12841 ttatttactc ttttatctta agaaaaactt aactaaaata tagcgaaatt cataaaaatt
    12901 taggaaatct taagaattat aatatttaaa tctttagatt tatattttat tatataaact
    12961 atagaataat aaaatataaa aattgagaat tttataatat atttgtagat ttgagtataa
    13021 aaaagtgcta agttgaaagg agtaaaatat ggataaggtt aatttagaaa ggtatagttg
    13081 tgatataaat gttggtttaa ataaaaatca acttcaaagt agaatagatg ataatttaat
    13141 taatgcattt gataatggaa aaaccaaaac atataaacaa atttttaaag acaatatatt
    13201 tacacttttt aatctcataa atattgtttt agtctgttta ttagtttcag ttggttcttt
    13261 taaaaatacc ttatttatat ttattgctgt aattaatact attataggag taatacaaga
    13321 gataagagct aaaagattat tggacaatct atctgttata gtttcaaata aggtatctgt
    13381 cataagagaa aaagagaaaa aagagattga tgtacaagaa ttagtattag atgatattat
    13441 gattttaaaa acaggtaatc aaatttgtgc agattcaaaa gttttaaatg gaaaattaga
    13501 ggtaaatgag tctttggtta caggtgaatc tgatattatt ataaaaaata aagacgattt
    13561 tttatattct ggaagttttg tagtttctgg agaagctttt gtaagagttg aaaatatagg
    13621 aaaagataac tatgcaaata agatagttaa tgatgcaaag atttctaaaa aacataattc
    13681 tcaacttaga aattcattaa attcaatatt aaaaattgta ggtataataa taattccttt
    13741 aggaattata ttgtttgcaa aacagcattt tggaaatgga gattctatag ctacatctgt
    13801 agtaggtaca gtggcagctg taaatggtat gataccagag ggattaacat tacttacgag
    13861 cattgctcta gctgtaggaa ctataaaact tgctagtcat aaaactttag tgcaagaact
    13921 gtactgtgta gagactcttg ctagagttga tacactatgt cttgataaga caggtacaat
    13981 tacagaaggt aaaatgcaag ttgatgatat agtaatgttg gaagaagtgg atattaatga
    14041 aataatgggg aatatatgta attctctcaa agatgataat gcaacactaa atgcaataag
    14101 agataagtat aatgttttag atacgtataa agctaagaat ttaataccat tttcttcaga
    14161 aagaaaatac agtggggtta cctttgaaaa taaaggtaca tatttattag gtgcatttga
    14221 gtttatattt aaggaaaaaa ataaaaaaat tagaatagaa gttgaaaaat attctaaaaa
    14281 aggaaacaga gtaatagttt tagctcatag tgattcttat atggaagata ataatattcc
    14341 tgaagatata aagccactag cttttatatt gatattagat aaaattagag atgaagctaa
    14401 ggaaacttta gaattttttt acaatgaagg tgtagatata aaaattatat ctggtgacaa
    14461 tcctataaca gtaagtgagg tagctagaaa agcaggtctt aaaacggcta gtaattttat
    14521 tgatgcaaca acacttaaaa atgataacga tatatacgaa gcagttgata aatacagtgt
    14581 ttttggaagg gtaagcccac aacagaaaaa acaaatgatt ttagcattaa aaaaacaaaa
    14641 tcatacagta gctatgactg gagatggtgt aaatgatgtg ctagcgctta aagaagcaga
    14701 ttgcagcatt gccatggcat ctggaagtga tgttactaag aatgtttcta atttagtctt
    14761 attagattca aactttgcat caatgccaaa ggttgttatg gaaggacgaa aggtaattaa
    14821 taacattcaa agagcatctt ctttgttttt agtaaagaca attttttcat tcttcttgtc
    14881 actattaaca ttattcttct tcagtagaca gtatccattt gttcctatac aattatcact
    14941 aattggagct gtaacagtgg gtataccatc attcttctta gctcttgaag caaacaaaag
    15001 tagaatttca ggaaactttt tactcaatat attaaaaaat gctttaccag gagcactatg
    15061 tgtgacagta aatgtaatta ttgtttacaa tatagttgaa tatttaggct atagttcaag
    15121 tgtattttct acaatgtgtg ctatattaac tggtgtatgt ggtcttttaa tattaagaca
    15181 acaatgtttg ccatttaata aagaaagatt atttttaatt gtaagtatga ctattgcatt
    15241 tgttattggg attctatttt taggtggatt attctcattt gtagcattga gtagagaatt
    15301 aattctcata actatggcat tagcaatatt aatgccaatt atgataaaag ttatgttatt
    15361 tgtcttggat gagatattag agacaattgt tccagattta aattaatttt aaaatgatat
    15421 aaataagagc agttttagtt gaactgctct ttttagatgt gtttaatttt atataagaat
    15481 atgtattttt attaatcagc tatatatgag caacagtagt caacaacttc attaaccttt
    15541 aaatcaaaac ttgaatcact aggaacatta aatttttgcc cttctttata agttacaaac
    15601 tcattgcttc caggcagttt tacatccata cttccagcaa gcatctccat tatttcttct
    15661 tctctagtag aaaaagtata ttctcctggc aacattaatc ctaaagtttt tctttctcca
    15721 ttaggcagta ttattactct gctacttacc ttaccatcaa aataaacgtt tgccttcttt
    15781 actgcagtaa cattcttaaa ttcactcatt agataaaacc tccaaaatta tttgtacata
    15841 tatattatat aaaaataatg taaaaaatgc tatatatttg caaaaaaaat ttagtattta
    15901 ttaaaaaaat taataagaat aaaaaagaga tgaaattgct taaattgata aaaaatataa
    15961 aataaattgc aaagtaagca agaaaagaat aagttgaaga gttatatatt ataaatatta
    16021 aaaattttag tattaataaa aaagatatta cattaatatg aaaatatagg agattagaag
    16081 tgtttgataa ttacagttta tatattataa taaaataaat tgttaatttt aaaagaggtg
    16141 aattttatgg cacatatatg ctttgaggat atttgttatt tgttattaaa agaagcaatg
    16201 aagttaaagt taataccaca taataaaatt gtattatttg aagatgattt atcaataggt
    16261 aatatttttg atgtaaataa ctacgaatta agagaaaata tttttaagat tagaaatgtt
    16321 tttgaaaata atccgaaact attattcaag ccagatttgt tagaagatag gaaattatca
    16381 aaatgtagaa atataataat atggtgtgca aataacccaa aagatttgtg tggagtatat
    16441 tatgtagtga gtaagtttca agataaaaat ataaagattg cattagtaga tgaaaaaaag
    16501 acagaagatg ctctaattag atattcattt accagtgaaa tgtctccaga agattttcca
    16561 ttctttttag ataagacact agaaattggc tttaatgaaa aactagaatt taaaaataaa
    16621 tgggatgtgt tagttaaaga aaattctatt tttagagcat tagtaggtaa cgaaattaaa
    16681 agtgttgaag aaacttattt tgataaatat atattaagaa atatcagtga caaatggcaa
    16741 cctttaattg aagtggttgc aaactgtatg tttgaagatg aaatgcgtgt aaaagattgg
    16801 tttatacttt ggagattaga gaagatggca agtttaaaaa tagtaaaaat aaaaggaagt
    16861 aaaggggatt tttattctga taaggagata agaaaagttt aaaaattgaa tttataataa
    16921 attgtattaa ataatgggta taactatgaa gaaaaatatc ttaacagtat taatattata
    16981 attagcaata agtataatat ttattctata atttgaaaat ataataacta ataccttacg
    17041 aataagtgtg ctaaataaaa aagaaatact tatttcaggg atattagtat atattttaac
    17101 tacattttta cttaatagta ttaaaatatg gtgctataga gtaaataaac tagaaaattt
    17161 caactttaat attacaatag tttattctaa atgccacttc tttagatata ggagatgaaa
    17221 agcaagtttt aatttttttt aatcaataca tgttagatat atcattgtaa attaacataa
    17281 ctttgaatat aataatgttc agaaggcaat atttttgagg tatgaatcca ataatatagt
    17341 cttatgattg tagatatcaa aaaatataag ttactaggaa tacgttttgt agatttatat
    17401 atgattaata actaaagcta taaataaaca aaataaaaag agtatgtttc aacctatagc
    17461 ttttagattt aaaatatact cttttattaa ttatagttgc ataaattaac cataatatat
    17521 aattaaaatt tttattataa ttatatatta ggttcttcaa ttagttttcc acatacatat
    17581 ctatgaatga tatttctatc atcaccaaca tagataaatc tttcaagtct ttcaagtata
    17641 gaataattgt catgatttaa atttgaatca tcaataatca atgcatcaaa atcataacct
    17701 tcttcaaagc taccaacttt tccaaagaaa cttccaccac ttttggttgc aatataaaaa
    17761 gcctcagata aagataagaa attatacttc ttaccagaat taacccaata taatttagat
    17821 aattgtattg cagataccat tgctttaaat attgacaatg tatgtccacc gcttatatct
    17881 gatccaagtg ctaatctaat attattatcg attaattttc tgataggcat agcaccacta
    17941 ccaacattaa agttagaagc tggacagtga actgccataa cattgttttc tctcattaaa
    18001 tctatttcat cctgagaaca atgaacacaa tgagccatta aagtaggagt ttgaccaaat
    18061 aaattatatc tattatatgc atctccatag aatttagatt caggttctag acttctaacc
    18121 cattcaatct catcataatt ttcagataag tgtgattgca taggaatatt atattttaaa
    18181 cataaatctc ctaaaccttt taaaagttca cttgtacagc ttggtataaa tctaggtgtt
    18241 attattggtt ttacaataga atctgtatca ctatacttat ttataatttc ttctgtatca
    18301 gcaatagaat cctgagtgtt ttctaataaa gtatcagggc aattcatatt catatttact
    18361 ttaccaacgt atgctccaag accaacttct ttgaatatat ccataagttt catagaggct
    18421 tctttatgta ctgttgcaaa cactgctatt cttgtagttc cagatttcca aagttctttt
    18481 acaaattttt tatacatttt tgttgcatat tctatatctt tataatttga ctcttctgga
    18541 aatgtataag tttcaagcca tggcaaaagc tctttatcca tagccattcc aagattttta
    18601 aattgagagg catgggcatg gaggtcattc ataccaggta ttataagctt atcagtgtaa
    18661 tctactactt ttaggttttt gtattcctta ggtatctctt taaatattcc ctttacttta
    18721 cctttttcca gtataatata actattttca aatactgtga aagtttctga agtttttgta
    18781 aaaattatat ttccttttaa tacttttaac atataggtct ccttagtttt aaatttgttt
    18841 taatatatgt aggtaaaatt ttgttttaca aatcaaaatt ttaccacatg tattatatca
    18901 aatctaaact ataaatacta ttagataata gcaaatatta gtgaaataca gttgtttact
    18961 attattatat ataactgaca cttaagtgac atttaagaaa aatataatgc ttacttacat
    19021 aaaatggaat gttatttaaa gagaactttg attatatttt caaaagcttt tttatccata
    19081 tcgtttaaaa caagaaaata tctattcata gttattttta tattagtatg tcctaatctt
    19141 tcagagatga tttttatatt agttccagct agaagaagaa ttattagaat agataatata
    19201 gtaagtattt acaaatatgt aggtgttctt aaattgataa attattccat tttaatttta
    19261 tagtttgaat tttatgatat aataaaaata tataaatttt gcagtgagcg atatttttga
    19321 taaagtaggg tttaacagtt gcaatgtaag ggattgaggg tgtatgataa atgttatcaa
    19381 ttgcactact catggttcac tgcaaatttg agagagttgt atgtgtgtaa gtactgaaaa
    19441 tacttagttt attttggggt tttatattaa ctatgtggta tgtaaattta atttatctat
    19501 aatttaaaac ggtgaattac atatgtttta tattaactat gtggtatgta aattaaagag
    19561 gggtacttcg tacttttacc actcaaacta gttttatatt aactatgtgg tatgtaaata
    19621 ggtcaagcat agattttgca actggaagat aagaagagtt ttatattaac tatgtggtat
    19681 gtaaatgttt tagaacagaa aaaattgtat aaattgtgga atggttttat attaactatg
    19741 tggtatgtaa atttgtaaaa tgtaaatagc atattaattt ttctttcacg ttttatatta
    19801 actatgtggt atgtaaattg gagcattatg tttttaggtt acggagcaac atttagtttt
    19861 atattaacta tgtggtatgt aaatggtgat agtggaagtt ttacttatac ttggcgtgct
    19921 ggttttatat taactatgtg gtatgtaaat ttcagaatac ttagctcctg cgcctatatc
    19981 gtctgaatgt tttatattaa ctatgtggta tgtaaataaa gttttgcgca ttgtatgtgt
    20041 gcctagatta tctgttttat attaactatg tggtatgtaa atatgtcccc acatcaattt
    20101 tcctccgcat gaaaaaatcg ttttatatta actatgtggt atgtaaattt tagaaacaac
    20161 agaataaaca taatatataa gcatagtttt atattaacta tgtggtatgt aaatctatat
    20221 cgcatattct tttatctagg tcttcttttc gttttatatt aactatgtgg tatgtaaata
    20281 taatcttgtt atagacttta ctattgttgt aatagagttt tatattaact atgtggtatg
    20341 taaatcttac cagaccctgc aacactaaat gacttacata gttttatatt aactatgtgg
    20401 tatgtaaatt catcagtatt tgtaaaatgc tttaacttat cataatgttt tatattaact
    20461 atgtggtatg taaattttta cttttccttt ctatgtgtcc aattcggaca cattgtttta
    20521 tattaactat gaggtatgta aatcttatac ttagttagaa ctatatatcg acacaaatat
    20581 gctttatatt aactacatga tatataaatt gcaattttta ttcgttgtcc aatcgcttga
    20641 aatttgtttt atattaacta cgtggtatgt aaatttgcaa caagtatagg taaaataccc
    20701 caataattta tacagcattt tctactttaa aatataatta ttttttatca tttgtagtaa
    20761 ataattacca gataacattg actttagttt taatgattaa aatataaaag tagaataatt
    20821 ataaaaagta ttgaaaagtt tataaatata tataataaaa cttaatgaca agatattaga
    20881 tataaaaaat aattatctta taaatagatt gaaatttatg aatattcata ctataattta
    20941 aatataaaga gatgtccttt aaaaataaaa attaaaaaat atttaatgct actacaatag
    21001 gaactagaac tgcacttaat aaatatacag aaattagatt tggctcaata taaaatacaa
    21061 atatagaatt taggtgtttt ttatgaaaaa aattttatat gctttatata gtttcattgt
    21121 tattatagct aattttagat taaaagaaaa aaattataac tttattttat tagcaatctt
    21181 ttttatactg ctgttaattc aaaatggagt tttaaaaaca ggttatcaaa atagagctac
    21241 attttatcaa gataattatt ttaatggaga ggataaaaat gataagataa aatttataac
    21301 tataaagatt tttatatttt tgtcattacc attatgtttt aatatactat tcaattattt
    21361 aacttcttaa attatattta gatataggag tgttatgata aaagtaagat tatgtgttgt
    21421 ttatattaaa tataaagtgc atatagtgat aaaacaaaag tttcatataa atgaagaaaa
    21481 aatataaaat aatttattta actacttaaa aataaactaa aagggataat aaaatagctt
    21541 ttaggttatg gtaataaagg tatttaaaaa gagtgcataa gcactccaat tgtgagaaat
    21601 acatacacaa atcattatta caacaatgaa aaatgtacat ataagagacg ttttgtattt
    21661 gttttaagtg ataatatttt aatactaact aaataattat cacaataata aaaaagaaaa
    21721 aggagtattt tgtactccat ttgtcaaaaa tataaaactt ttatataagc ttattataac
    21781 ataattgata aaagtgtaaa gctatataat gaatttataa agaaaaataa aataaaaaag
    21841 ataaatccta atcaaaggat ttatcttttt gtatagaaaa taattattct attattaaat
    21901 ggcatggcta aactggataa taatttatta ttactgcttt caatgttttt tctccttgat
    21961 tatggtatgt atgaattgat tcagctgaaa aacaaataga gtcattttct ctaagttgat
    22021 aaatagagtc atttacttct aattttaatt gaccttctat aatcataata tattcctgtg
    22081 atttttcaga atgaccaaca gatgtataac tatgaccttc ttctaattcc atttgaaata
    22141 attcgaagtt tctagttggt gtattgggat aataacaata aagacgataa tctttatttt
    22201 ctgaaacttg tacatctata tcagtttttg atacaataaa agtttcattt tgaggttgtt
    22261 ccagtattgc agtatatgga acatttagtc cacttgcaat tttccaaatt gtgtttactg
    22321 ttgggtttga gtctcccttt tcaatttgcg aaagcattac tttacttacg ccagataatt
    22381 cagcaagttg acctagactt aagttacgtt cattacgtaa tctatttaaa tttttagcaa
    22441 taacagtatt aatactcata gtaactcctt aaaatgtaaa ttattgacaa atataattta
    22501 cgaatgtata atttatttaa cattatttat aatttacaaa tgtataatat aatgaacgtt
    22561 aaatacactg tacatccgta atataaatta tactgcataa agagttgttt ttcaacggat
    22621 gatagttgag agattaaggg ggaattgtaa tgcgtgtact taataatgtt acagaggcta
    22681 taggcaatac tcatatgtta catttatcca aaatagcaga tgaatttgga gttcaaggta
    22741 atatttatgc aaagatggaa tacttaaatc cgggatttag taaaaaggac cgtgtagcat
    22801 tacaaatgat taccgaagca gaaaatgcag gtttattagt tccaggtcaa gcagttgtgg
    22861 aattaacaag tggtaatact ggaacaggac tagcaatagc ttgtgcatgt aaaggatata
    22921 aatttatagc gtgtatgtca aaaggaaatt ctatggaaag agcaagaatg atgaaagctt
    22981 taggtgctga agtagttctt gtagaccaaa tgcctaattc agttcacgga caggtatctg
    23041 gtgaagattt ggcacttgta gaaattgagg ctcaaaaaat agcaaaagaa agaaatgcat
    23101 ttagagcaga ccaatttaat ttagaagctt gtaatcatgc acatgaaaag tatacagcag
    23161 aagaaatgtg ggaacaattg gatggaaata ttgatgtatt tgtagatttt cttggaagtg
    23221 gtagtacttt tgcaggatgt tcaaaaacac ttaaaaaaca taattcttct atcaaatgtt
    23281 atgttgttga acctgaaaca gcagcaattt attcaggtaa agaaataact gaccaagggc
    23341 ataaaataca aggtggagga tattctatgg atttgccatt agtagacaag aaacttattg
    23401 atggttatat tcaagttaca gacgaggagg caactgatgt agcaagaaaa ttagctaaat
    23461 tagaaggtgt atttgcagga ttttcttcag gggcaaatgt gtgtgcagca attaagttgt
    23521 taaagagcag tgaaaaagga aaaaatatag ttttaactct taatgatagt ggtttaaaat
    23581 atttaagtac tgatttatat gaagaattgt aataatagca caatagttat gcaatgtttt
    23641 ttaaattaag tatatagatt taaattataa aaagtatctc aatgtgattt ttgaaagtta
    23701 taaaagagat acttttttat tttgtaaata tggagtatcc tacttataag tattaagcta
    23761 tatagatatt gatgattagc tatgcaataa aaaagagtat gtgtttttgc atttatacat
    23821 cataacttgg aataatacat attaatgttc taaatgtatt taaaacatat gataaaatta
    23881 gaacaaacag atacaaggag ataaacatgc aaaagagaat tttaagaaat tttatattta
    23941 cgggtaaaaa tattttaagt aaacttgttt ttataataaa atataatttt agtacattaa
    24001 ttttatttga attatttcat aaaggattag ccttattttt tatatggcca tctattagat
    24061 atatatttga tattttaata aaaagtttac atatagtcta cctaaatatg gataatttta
    24121 taaaagtttt gaccaatccc atgtcaatag ttctgataat actatcagtt ataatactag
    24181 ctttttatgc attttttgaa tttacctctg taattatttg ttttaataaa tcgataaaat
    24241 atgaaaaaat agggttattt gaactcttta aaatatcatt taagaactct ataaaattgt
    24301 tatatcctaa aaatatacta ttatttatat ttgtgatatt aataattcca ttagtaaata
    24361 cagttttaat ttcagggttt ataggaaaaa tacaaatacc agagtacata ttagattata
    24421 taaagtctga tataatttta aatataatct atatgatttt tcttttaata atctatattt
    24481 cggttattag atggattttt agcattcatg agataacttt aaatacagaa aattttaaaa
    24541 gagcaagaaa agaaagtgtt aatcttataa agagaaagat tataagaaca attatttatt
    24601 cattaatatt atctttagta gtagctatta ttggatatgc gatttatcat agtggaatta
    24661 tatttatagg catatggact aaatattgta ctgatatgga atttttgaag aatgtattta
    24721 taaatagatg tattgcattt gaagaatatt ttagattgat tttatcaata ttcattttta
    24781 tattggacat tggatttatc tcagcaactt attatcaata taaaggagta aatatttcta
    24841 aaaataatat taaaaaagag aaaaaatcta tatcaaaaat tgtttttaat ttagtattaa
    24901 tattattgat ttcttatata gagtcagttg cattttcttc acatataaat ggcatgttta
    24961 atacgtcatt ttcattttat acaacagcaa ttgctcatag agcaggagct gaaatggctc
    25021 cagagaatac tttggcagca atagaagaag cttcaaatgc taaagctgat tatgcagaaa
    25081 tagatgttca agaaactaaa gatggagaac ttattgtact tcatgattca aactttaaga
    25141 gaactacagg agtagataaa aatgtttggg atgtaaatta taatgaagta aagacatatg
    25201 atgcgggaag cttttattat tatggaaaag gttattatga tgaaaagttt gttggggaaa
    25261 agataccaac actcaaagaa atgataaaat attctaaagg taaggtgaaa ttattaatag
    25321 aaataaaact taatggacat gaaaagggag acatagtaaa aaaagttgtt cagttaatta
    25381 aagaaaataa ttttgaaaat caatgtatag ttgcatctat ggataaaact atattacaaa
    25441 aagtaaaaaa actggatgag aatttactga catgctattt tatggcaatt gcatatggtg
    25501 atttttcaaa gttaaattat gttgatatat atggaattga atcaactttt gttaataaaa
    25561 cagtagtaga aaagattcat aaaatgaaca aaaaaatctt cgtttggact gttaatagtg
    25621 acagtttaat tgagaaaatg cttgacctaa atgtagatag tattattaca gataatccat
    25681 accttataga gagtgcaatt tattggaaga aaaatggatt tattagtcaa gtgtcagatt
    25741 atttatttta aatattctat aattgtatta tccagtaaaa gagatattat taaaatcaat
    25801 aatatctctt tttaacataa ataaggttgt taaattaagg ttattcttat aaaaattgtg
    25861 cttatcagct ataaacaagg aataaaagtg aaaaaattgc tctgagatta ttacagttaa
    25921 aagagtttta agatgacaga gagatagatt gtcaactgta tatttaataa atcttgttat
    25981 ttattaaatt ttatataagt aaaatatata aattaataaa tcttatgcta gaatatatat
    26041 gaaaaacaat ataaatttgg aggattaaaa acatgacaga gcgagaaaaa atgttggcag
    26101 gagaacttta cgattgtggt gatattgagt tgttaactca atggcataaa gcaaaagacc
    26161 tagtaagaga ttataaccaa acagattcct cggatttgga aaaaaaagaa aaaatattaa
    26221 atgaattact tggtggtaag gataaaaact tatggattac tgcaccgtat tatgtagatt
    26281 atggaaataa tatttatttt ggaaataatt gtgaagtcaa tatgaattgt acgtttttag
    26341 atgataataa aattatcatt ggggataatg cactaatcgc accaaacgta caaatatata
    26401 ctgcatttca tccaactaat gctggtgata gatttggtaa tgtcaaagaa gatggttcat
    26461 ttgaattttg taaaactcaa acagctccag ttactattgg agataatgtt tggattggcg
    26521 ggggtgctat tatcatgcca ggtgttacta ttggagataa tgtagttata ggtgcaggaa
    26581 gtattgttac aaaagatatt ccaagtaata tgattgcata cggaaatcca tgccgtatta
    26641 ttcgagaaaa caaataaagt taaattttct tgcaaaatct caaaaaacac ctttcttact
    26701 ttatacatag ggtattttcc aatactttca catccatatt catttgttaa atttttgtgg
    26761 aatgtagttt ttatcacttc tatcttacat ttccaccatt ttttataaaa gtcattcctt
    26821 tatagattta tttataatag tatatattct atttgatgtt tgaataagca cttattttta
    26881 agtaaaatac taacttttag gttagtattt tgctatagta tgatgattaa gtaagaataa
    26941 aatact
//
LOCUS       DAMKPG010000049        26376 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMKPG010000049 DAMKPG010000000
VERSION     DAMKPG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.052"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            295..753
                     /locus_tag="PC621_002144"
     CDS             295..753
                     /locus_tag="PC621_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4109297.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            814..1089
                     /locus_tag="PC621_002145"
     CDS             814..1089
                     /locus_tag="PC621_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4109298.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            complement(1817..2149)
                     /locus_tag="PC621_002146"
     CDS             complement(1817..2149)
                     /locus_tag="PC621_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109299.1"
                     /translation="MQEAIIKLKLLGQMPDAVKDDPTEETINMYDELLSNVKTPLTRE
                     EVGVLIDIFPEGGMYGVEWDLLKLVESYLIEAPSSEEYRKLITACPSEEWRETMQARL
                     DNWENNKQ"
     gene            complement(2176..2976)
                     /locus_tag="PC621_002147"
     CDS             complement(2176..2976)
                     /locus_tag="PC621_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109300.1"
                     /translation="MELSIQERLKDLRVERGLTLEQLAEETHLSKSALGSYEGDNLKD
                     ISHHALIQLAKVYEVTVDYLLGRSKTKNHPNADLADLRLSDDMIELLKSGRVDTSLLC
                     ELAVHPDFPRLMADLEIYVNGTAVKQVQSANAIVDIMSAAIMKQHNPGLSDPQLRQLI
                     AAHIDDDSFCRYVIQQDINKIALDLREAHKDDFFSVPEDNPLEDFLQTAEETAKEGSD
                     PEQASLAFICKRLKLNLKKLSEEEKKWLKKIAQKSDLLKNPNPQRGRK"
     gene            complement(3128..4162)
                     /gene="cfr(E)"
                     /locus_tag="PC621_002148"
     CDS             complement(3128..4162)
                     /gene="cfr(E)"
                     /locus_tag="PC621_002148"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105119688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(E)"
                     /protein_id="HDF4109301.1"
                     /translation="MIYSKYEVMKRCISGMNFPDYRYEQLIKMIFAQHIPDFHSMYML
                     PERLRSNLAETFGTSVCGLVPVTHRASGQADKVLFQLKDGNCVETVNLHYKKGWESFC
                     VSSQCGCGFGCKFCATGAIGHKRNMTADEITDQILYFYLAGHQINSVSFMGMGEPFAN
                     PNLFDALKILTNPSLFGLSQRRITISTIGMIPGIKRLTRDFPQVNLAFSLHSPFEKQR
                     SELMPINQTYPLHKVMDALDAHVANTKRRLFLAYIMLGGINDSAEHAKALAHLILSRG
                     ALSYLYHVDLIPYNATDKTARKFTASNHEAIKNFSDILHSNRISVAIRMQFGSDIGAG
                     CGQLYADERD"
     gene            complement(4180..5658)
                     /locus_tag="PC621_002149"
     CDS             complement(4180..5658)
                     /locus_tag="PC621_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HDF4109302.1"
                     /translation="MSLINISNLTFSYEGSFHNIFENVSFQIDTDWKLGFTGRNGRGK
                     TTFLKLLMGKYEYSGTISSNVSFEYFPYDVPDADFFVIDVIHEISPNAQDWEIARELS
                     LLNVSDDLLYRSYSTLSKGEQTKALLAALFLKENSFLLIDEPTNHLDTLGRKTLSDYL
                     KRKHGFILVSHDRVFLDNCIDHILVINKTNIEVQRGNFSSWWRNKEMQDSFERAENEK
                     HKKEISRLTAAAQKTSGWSDRVEKSKNGSTNSGSKLDKGYVGHKAAKMMKRSKAIENR
                     QQDLIHEKSKLLKNLETAESLKLSPLPYHTSRLLELSDVSIAYGSRAVCSNISFTIEQ
                     GDRIALQGKNGSGKSSILKLICGENIPYQGILRKSERLKISYVPQSTAGLNGFLSEYI
                     ERNLLDESLFKAILRKFDFPREQFEKRLDEFSEGQKKKVLLAKSLCEQAHLYIWDEPL
                     NYIDVLSRMQIENLLLEYKPTILFVEHDSAFCENIATKTVEL"
     gene            complement(5958..6404)
                     /locus_tag="PC621_002150"
     CDS             complement(5958..6404)
                     /locus_tag="PC621_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4109303.1"
                     /translation="MEIIEIKENKKQFLPLLLLADEQESMIDRYIDRGMMYVLVDNGV
                     KCECVVTDEGKGILEIKNIATAPEYQGKGYGKALIDFVAAKYKGKYSILQVGTGDSPL
                     TVPFYEKSGFVRSHSIKNFFIDNYDHPIFEGGVQLTDMIYLQKNIL"
     gene            6782..7195
                     /locus_tag="PC621_002151"
     CDS             6782..7195
                     /locus_tag="PC621_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDF4109304.1"
                     /translation="MRENPYKRLPPIERKPDGSLYRMTPAQRKQANALIRRECCNYED
                     GNCMPLDDGDTHTCPQTISFSVCCKWFRWSVLPQVGTLEAEIFRDKELKRCAVCGRVF
                     VPKSNRAKYCPGCAARVHRRQKTESERKRRSTVDS"
     gene            7307..7702
                     /locus_tag="PC621_002152"
     CDS             7307..7702
                     /locus_tag="PC621_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDF4109305.1"
                     /translation="MINTIYIHQPEKSVSFTRLPNFLFEAPTFTALPNEAKLLYAFIL
                     RRTELSRKNGWADEYGRIYLYYPIYEVVELLHCGRQKAVNTLRELQYAGLVEIQKQGC
                     GKPNRIYPKSYEAVPNTDFKKSRSGTPED"
     gene            7885..8829
                     /locus_tag="PC621_002153"
     CDS             7885..8829
                     /locus_tag="PC621_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDF4109306.1"
                     /translation="MAQHAILRFEKHKGNPARPLEAHHERQKEQYASNPDIDTSRSKY
                     NFHIVKPEGRYYHFIQNRIEQAGCRTRKDSTRFVDTLITASPEFFKGKSPKEEQEFFQ
                     RAADFLIDRVGRENIVSAVVHMDEKTPHLHLVFVPLTEDNRLCAKEIIGNRANLTKWQ
                     DDFHAYMVEKYPDLERGESASKTGRKHIPTRLFKQAVNLSKQARAIEATLGDITPFNA
                     GKKKEEALSMLKKWFPQMENFSGQLKKYKVTINDLLAENEKLEARAKASEKGKMKDTM
                     ERAKLKSELDNLQRLVDRIPPDILAELKRQQRQHGKER"
     gene            8899..9090
                     /locus_tag="PC621_002154"
     CDS             8899..9090
                     /locus_tag="PC621_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109307.1"
                     /translation="MEHIRYKKETEVVTFQGKEITLENLSPVFTPDQEAAKRRELEQQ
                     LYEVFRKYADKRQKEEAGA"
     gene            9185..10696
                     /locus_tag="PC621_002155"
     CDS             9185..10696
                     /locus_tag="PC621_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4109308.1"
                     /translation="MNNRIDAIYARQSVDKKDSISIESQIEFCKYELKGGNCKEYTDK
                     GYSGKNTDRPKFQELVRDIKRGLIAKVVVYKLDRISRSILDFANMMELFQQYNVEFVS
                     STEKFDTSTPMGRAMLNICIVFAQLERETIQKRVTDAYYSRSQRGFKMGGKAPYGFHT
                     EPIKMDGINTKKLVVNPDEAANIRLMFEMYAQPTTSYGDITRYFAEQGILFNGKELIR
                     PTLAQMLRNPVYVQANLDVYEFFKSQGTIIVNDAADFTGMNGCYLYQGRDVKPSKKND
                     LKDQMLVLAPHEGIVPSDIWLTCRKKLMNNMKIQSARKATHTWLAGKIKCGNCGYALM
                     SIFNPSGKQYLRCTKRLDNKSCPGCGKIITSELETVVYQQMIKKLASYKTLTGRKKAA
                     KANPKIAALQVELAHVDGEIEKLVDSLTGANNVLLSYVNVKIAELDGRKQELLARIAE
                     LTVEAISPEQVSQISGYLDTWEDVSFDDKRRVVDLMITTIAATSDSLNITWKI"
     gene            10744..11925
                     /locus_tag="PC621_002156"
                     /pseudo
     CDS             10744..11925
                     /locus_tag="PC621_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430710.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            12055..13107
                     /locus_tag="PC621_002157"
     CDS             12055..13107
                     /locus_tag="PC621_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF4109309.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            13107..14138
                     /locus_tag="PC621_002158"
     CDS             13107..14138
                     /locus_tag="PC621_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF4109310.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            14230..15042
                     /locus_tag="PC621_002159"
     CDS             14230..15042
                     /locus_tag="PC621_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF4109311.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            15045..15965
                     /locus_tag="PC621_002160"
     CDS             15045..15965
                     /locus_tag="PC621_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF4109312.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            16140..16586
                     /gene="rpiB"
                     /locus_tag="PC621_002161"
     CDS             16140..16586
                     /gene="rpiB"
                     /locus_tag="PC621_002161"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF4109313.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            16625..17290
                     /locus_tag="PC621_002162"
     CDS             16625..17290
                     /locus_tag="PC621_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF4109314.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            17400..18902
                     /locus_tag="PC621_002163"
     CDS             17400..18902
                     /locus_tag="PC621_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF4109315.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(19214..20806)
                     /locus_tag="PC621_002164"
     CDS             complement(19214..20806)
                     /locus_tag="PC621_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4109316.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INCYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYSEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(20775..21491)
                     /locus_tag="PC621_002165"
     CDS             complement(20775..21491)
                     /locus_tag="PC621_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109317.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(22032..22832)
                     /locus_tag="PC621_002166"
     CDS             complement(22032..22832)
                     /locus_tag="PC621_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF4109318.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(23147..23878)
                     /locus_tag="PC621_002167"
     CDS             complement(23147..23878)
                     /locus_tag="PC621_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109319.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(23865..24560)
                     /locus_tag="PC621_002168"
     CDS             complement(23865..24560)
                     /locus_tag="PC621_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4109320.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(24562..25353)
                     /locus_tag="PC621_002169"
     CDS             complement(24562..25353)
                     /locus_tag="PC621_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109321.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(25363..26175)
                     /gene="modA"
                     /locus_tag="PC621_002170"
     CDS             complement(25363..26175)
                     /gene="modA"
                     /locus_tag="PC621_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4109322.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         9027 a         4343 c         4971 g         8035 t
ORIGIN      
        1 ttgttaaaca taaaaaacca tttgtaaaaa acaaaaaaat catttctcta acaaaaaaaa
       61 ccacaaaaaa tataaaaaag tatggctagt taagtgatta acaagtaaaa attaaaataa
      121 aaatcaaaaa aaatttatca atgaaagtca gctaaaatcc tggaagagag cattattttc
      181 gggaaaacat tttcttttgt ttcacaaaaa aagccacttt ttggcacgca acatgcttta
      241 tataaagtca agaaaaacta aggtataaga caaaggcaat taggaggtgt aaatatggag
      301 tttataaatt ttgtaagtga taaagttata gaccttaaaa tggattgtaa aacgaaagaa
      361 gatttcttta aagaaataca taataaagtt tttgatttag gctttgtaaa agaagaattt
      421 ggagaaaaaa tattagcaag agaaaatgta tttccaacag gtttaaattt aggtgattat
      481 ggagtagcaa taccacatac agatgcagaa tatattaaag agcaatttat atcagtctgt
      541 acttttaatg aaccagtagt atttagttct atggaagacc aagatgaaaa agtacctgta
      601 aatttggctt tcatattagg acttaatcaa ccacacagtc aattatctgt attaacagaa
      661 ttaatgggaa ttatgcaaaa taaagagtta gttgataagt tgatgagttc tactgataaa
      721 gaagaagttt taaggacact aaagtcttta taaaaataaa aaagtactaa aaataaaaaa
      781 tataaaaata aaaattatta ggaggaaata aaaatgaaaa aaatattagt agcatgtgga
      841 gcaggaatag caacatcaac agtagtttgt gacaaggtag agagattagt taaggaaaat
      901 aatatacaag cagaagttat acaatgcaaa atagctgaat gtgcaacaaa gcaagaagga
      961 gcagatttaa tagtatcaac aactatacta ccaacaactt ataatatacc agcaataaaa
     1021 gcaacagcat atataactgg agtaaataca gcagcattag ataaaaaaat attagatgct
     1081 ttaaaataat cctatcatag atacattgaa ataatatttt ataaaacaga aaaaggagga
     1141 tttataaatg gataaattat tagcagtagt acaatacatt ttaaatatgg gaccgacagt
     1201 aatactacca ataacaattg tagtactagg aatgatattt agaattaagt ttaggtcagt
     1261 ttacaaggaa ctacacgcag accacgaaac gggggctgct gacaacaaat aaaggctgaa
     1321 tatcaagcac cgggaaagtg cgagatattc agcctgtttt gttgtcaggg gtttccaaag
     1381 gggtttgccc cttggcacac gactttgctc gcaaagtgta gtgtgttata cgctctgtcg
     1441 gcgttgctgc gaaaatgcgg ttgccgccgg ggagggcaag ggcattttta cggcaggaaa
     1501 ccagggctgt gattgcgtgg cgtggagccg caagcgcagg actggtttca tcagcagaca
     1561 gggcgtatat gaatgccccc gtccctcgtc ctacgccgaa ctatgggaca aagtgtccca
     1621 gacggatagc cacacttacg aaaaagcaga cggatttttc tctcaaaatt gaaatgggcg
     1681 ggaagccatt ttagggcttt cccgccttga ttacccatta gcaaagacgg gcggggctgt
     1741 caacggcggc gcatgaaatg cgccgttcat cttgaccgtt gactggctcg actggctttg
     1801 ctatctcccc aacaagttat tgtttgttgt tttcccaatt atctaaacgg gcttgcatag
     1861 tttctcgcca ttcttcactt ggacaagcag taatcaattt tcgatactct tcacttgatg
     1921 gtgcttctat cagataagac tctaccaatt ttagcaaatc ccactctact ccatacattc
     1981 caccttcggg gaaaatgtca atcaatacgc ctacttcttc tcttgtcaat ggcgttttta
     2041 cattggagag caattcgtca tacatgttga tggtttcttc tgtagggtca tctttcactg
     2101 cgtctggcat ttgaccgaga agtttcaatt ttataattgc ctcctgcacc tttactccct
     2161 ccaatttctc gttctctatt ttctcccccg ctgtgggttt ggatttttca gcaagtccga
     2221 tttctgcgca atctttttca gccacttctt ttcttcctcg gacagcttct tcaaattcag
     2281 tttaagccgc ttgcagataa acgccagcga cgcttgctcc gggtcgctgc cctctttagc
     2341 ggtttcctcg gcggtctgta aaaaatcttc cagcggattg tcctctggga cgctgaaaaa
     2401 atcgtcctta tgggcttccc gcaggtcaag agctatcttg tttatgtcct gctgtatcac
     2461 atagcggcaa aagctgtcat cgtcaatatg agcggcgata agctgtctta actgcgggtc
     2521 ggacaagccg ggattgtgct gcttcataat ggctgcgctc ataatatcca caatggcatt
     2581 tgcgctctgt acctgcttga ctgccgttcc gttcacatag atttcaaggt cagccatcag
     2641 ccgggggaaa tccgggtgta ccgccagctc acacaagagg gaagtatcca cccgcccact
     2701 tttcaatagt tcaatcatat catcactcaa gcgcaggtct gcaagatcgg cgtttgggtg
     2761 attttttgtt ttagagcgtc ccagcaggta atcaacggtc acttcataaa cctttgccag
     2821 ctggataagg gcatggtggc tgatgtcttt gagattgtct ccctcataac tgcctaaagc
     2881 agatttggaa agatgagttt cctccgcaag ctgttccagc gtcagccctc gttccacacg
     2941 caggtctttc aatcgttcct gtatggatag ttccatgcta tcacctcctt gtctgctcga
     3001 taaatgggaa tttatatttt tgatgtaaca ataaggcacc cagcattaaa tttatgccgg
     3061 gtgcctttcc tttcaaacgc caatgctgct tggaaatctc tgtgtacata aaaacgtctt
     3121 gaagcgctca atccctttca tcagcataca actgcccgca cccggcgccg atatcagaac
     3181 cgaactgcat tctgatagcc acgctgatac ggttggaatg taaaatatca ctgaagtttt
     3241 taatggcttc gtgatttgaa gcggtaaatt ttcgcgccgt cttatctgtt gcgttatatg
     3301 ggataagatc aacatgatat agataggaca acgctccccg gctcaaaata aggtgcgcta
     3361 acgctttcgc atgctctgcc gaatcattta ttccgcccag cataatataa gccaggaaca
     3421 agcgcctttt ggtatttgca acgtgcgcat ccaacgcatc cattacttta tggagcggat
     3481 aagtttgatt gatcggcatc aattccgaac gttgcttttc aaaaggcgag tgcagcgaaa
     3541 aagctaaatt cacctgcgga aaatcacggg tcagccgttt tatcccggga atcattccaa
     3601 tcgtcgatat ggtaatgcgt cgctggctta atccgaacaa agacgggttt gtcaggattt
     3661 ttaatgcgtc aaataaattg ggattcgcaa acggctcgcc cattcccata aaagaaacac
     3721 tattgatttg atgccctgca agataaaaat acagaatttg atctgttatt tcatcagcgg
     3781 tcatattgcg tttgtgtccg atagcacctg ttgcgcagaa tttgcagcca aacccacagc
     3841 cgcattgaga tgagacgcaa aaagattccc atcctttttt gtaatgcagg ttcaccgttt
     3901 cgacgcagtt tccatccttt aattgaaaaa gcaccttatc tgcctggccc gatgcccgat
     3961 gtgtaactgg aacaaggccg cagacagagg tcccaaaagt ttccgccaaa tttgatctta
     4021 gtctttccgg cagcatatac atggaatgaa agtcaggaat atgctgtgca aaaatcatct
     4081 ttatcaattg ctcatagcga taatccggaa aattcattcc cgatatacat cgcttcataa
     4141 cctcatattt tgaataaatc atgtaggctt ttctcctttc tataactcaa cggttttggt
     4201 cgcaatattt tcacagaaag cactgtcgtg ctccacgaac agaatggtcg gcttgtattc
     4261 gagcagcagg ttttctatct gcatgcggga aaggacgtca atgtagttga gcggttcatc
     4321 ccagatatac agatgcgcct gctcacacag gcttttggcg agcaggactt tctttttctg
     4381 cccctcgctg aattcgtcca accgcttttc aaactgctcc cgaggaaaat caaatttccg
     4441 caggatggct ttgaaaagac tttcatcgag aagatttcgc tcgatatatt cgcttaagaa
     4501 gccgttcagc cccgccgtgc tttgggggac ataggaaatt ttcagccgtt cgctttttcg
     4561 caaaatgcct tgatagggga tgttttcccc gcagatcagc ttcagaatgc tagattttcc
     4621 ggaaccgttt ttcccctgca gcgcaatccg gtctccctgc tctatggtaa aactgatatt
     4681 cgagcagacg gcacgacttc cataggcaat ggaaacatcg gaaagctcaa gcagtcggct
     4741 ggtgtggtat ggaagcggag aaagttttag gctttcggca gtttccaggt ttttgagcag
     4801 tttggatttc tcatgaatca aatcctgctg acgattttca atagctttgg aacgcttcat
     4861 cattttcgct gctttatggc cgacatagcc tttgtccagc ttgcttcccg agttggtcga
     4921 accgtttttg cttttttcca cacggtctga ccaaccggaa gtcttttgtg cggcagctgt
     4981 taaacggctg atttcttttt tgtgtttctc gttttctgcc cgttcaaagc tgtcctgcat
     5041 ctctttgttc ctccaccagg acgagaagtt tcccctctga acctcgatgt ttgttttatt
     5101 gattacgaga atatggtcga tgcagttgtc caggaaaacc cggtcatgag aaaccagaat
     5161 aaacccatgt tttcgcttca gataatcgct caaggttttt ctgcccaaag tgtcaagatg
     5221 attcgtcggc tcatcaatca gcagaaagct gttttctttc aggaacaaag cggcaagcag
     5281 tgcttttgtc tgctccccct ttgaaagggt ggaataggag cgataaagaa gatcatccga
     5341 cacattcagc agggacagct cacgtgcaat ttcccagtcc tgcgcattcg ggctgatttc
     5401 atgaatgaca tcaatgacaa aaaagtcagc gtccggcacg tcatagggaa aatactcaaa
     5461 cgaaacattg cttgaaatgg taccgctgta ttcatatttc cccatgagaa gctttagaaa
     5521 agtcgttttt ccccggccgt ttcgcccggt aaatccaagc ttccaatctg tatcaatctg
     5581 aaagcttacg ttttcaaaaa tattgtgaaa gcttccttca taggaaaagg ttaaattcga
     5641 aatattgata agtgacatca gtcaaacctc cgcaataaaa atatgggatg caagaaagtt
     5701 acttcctgca tcccatattg atagcgaagt caaagccgca taaaggcggc aaaacaaggc
     5761 tcatatgagt gaatcggcat taaactttct tgcaaacgca cagcaaaaac atcggcatag
     5821 cgtgtatgcc cgaagattca tgccatgcaa aaatagttag caagaaaatt taatcacctt
     5881 tccacctcaa atctaaaata atattatatt ttaatataca ccaaaacggt ggtaaagtca
     5941 atgctttgcg ttggatttca taaaatgttt ttctgtaaat aaatcatatc tgttagttga
     6001 accccacctt caaaaatagg gtggtcatag ttatcaataa aaaaattttt aatgctgtgt
     6061 gagcggacaa aaccagattt ttcataaaaa ggaacggtca acgggctatc ccctgtacca
     6121 acttgtagta tcgaatactt ccctttatat ttcgcagcaa caaagtcaat tagtgccttg
     6181 ccgtaacctt tgccctgata ttctggcgca gttgcaatat ttttaatttc aagaatgcct
     6241 ttgccctcat cggttactac acactcgcat ttaaccccat tatcgactaa tacatacatc
     6301 attcctctat caatatatcg gtctatcatg ctctcttgtt cgtcggctaa cagtaataag
     6361 gggagaaatt gttttttatt ctctttgatt tctataattt ccatatcggt atgtactcca
     6421 aaattatcgt ttattgttct gctttttcat tctaccacaa ttccgagagc gtggaaatag
     6481 gcagtttttt tggcggattt cctacctttc ggatatgcgg gacgggctgt cttttaggtg
     6541 tagacttagg gtagttcatc gatgagctgc accttgaaaa atgaatgacc gtccgaaaag
     6601 gaatacccag gcaggggaag caccgcaagg agccaacttc tggacacttt gtccagaggt
     6661 cacttcggga caacttgtcc cgaagctatg gggagcgtgc caacgccgga acacgccgca
     6721 ggaatggggc aggaagcctt ttcggagaga gcggcaacgg aaagcaaaat ggaggaaaca
     6781 catgagagag aacccatata aacgactgcc gcccatagag cgcaagccgg acggttccct
     6841 ttaccgtatg acaccggcac agaggaaaca ggcaaacgcc ttaatccgcc gggagtgctg
     6901 caactatgag gacgggaact gtatgcccct tgacgatggg gacacccaca cctgccccca
     6961 gaccatttct ttctcggtct gctgtaagtg gttccgctgg tcggttttgc cgcaagtcgg
     7021 aacgctggaa gcggagattt tccgggataa ggagctaaaa cgctgtgcgg tctgcggcag
     7081 agtgttcgtc ccaaagtcca accgggcgaa atactgtccc ggctgtgccg ccagagttca
     7141 caggcggcag aaaacagaaa gtgaacggaa aaggaggtct actgtggaca gttaggggct
     7201 gaaaagtcat tgatttacaa ggctttgcaa gcacagaaca ggggcaggca atagaaacta
     7261 ccgtctgccc ccggaaacag gcttctaacc gtccacaaaa cgcactatga taaataccat
     7321 ttatatccat cagccggaga aatcggtcag cttcacccgg ctcccgaatt tcctctttga
     7381 agcacccaca ttcacagctt tgcccaatga agcaaagcta ctgtacgcct ttatcctgcg
     7441 ccggacggag ttatcccgca agaacggctg ggcggacgag tacgggcgga tttacctgta
     7501 ttacccaatc tacgaggtgg tggagctgct ccactgcggg cggcaaaagg cagtcaatac
     7561 cctgcgggaa ctgcaatatg ccggactggt ggagatccag aagcagggct gtggaaaacc
     7621 caaccgcatc tacccaaaat cctatgaagc ggttccaaat accgacttca agaaatcccg
     7681 ttctggtacg ccggaggact gaaaaccgca ctccacaagt acgataatca atcctcttga
     7741 agtacgaaaa ccggacggta tatagaaata cagagataaa aatattgatt tatattctat
     7801 ccattccctt cctatccgag atattttctg cgggattttc ctgtggaaaa gccccggaag
     7861 ggaaaggaac ggggaaagga gtttatggca caacacgcaa ttttgcgatt tgaaaaacac
     7921 aagggcaatc cggcaagacc gctggaagcc catcacgaaa ggcagaaaga gcagtatgcc
     7981 agcaacccgg atattgacac cagccggagc aagtacaact tccacattgt caagccagag
     8041 ggacgctatt accactttat ccaaaaccgc attgaacagg ctggctgccg cacccgtaag
     8101 gatagcacac gctttgtcga tacgctgatt accgcaagcc cggagttttt caagggtaag
     8161 tccccaaagg aggaacagga atttttccag agggcggcgg acttcctcat tgaccgggta
     8221 gggcgggaaa atatcgtgtc ggcggtggta cacatggacg agaaaacacc ccacctgcat
     8281 ttggtctttg tcccgctgac agaggacaac cgcctgtgtg caaaggagat tatcggcaac
     8341 cgggcaaacc tgacaaagtg gcaggacgat tttcatgcct atatggtgga gaaatatccc
     8401 gacttggagc gtggggaaag tgccagcaag acaggcagga agcatatccc cacccgtctg
     8461 ttcaagcagg cggtcaatct ctccaaacag gcaagagcca ttgaagccac gctgggcgat
     8521 attaccccgt tcaacgccgg aaagaaaaaa gaggaagccc tctccatgct gaaaaagtgg
     8581 ttcccgcaga tggagaattt ctccgggcaa ctgaaaaaat acaaggtcac aatcaatgac
     8641 ctgttagcgg aaaatgaaaa gctggaagca agggcaaaag ccagtgaaaa aggcaagatg
     8701 aaagatacga tggaacgggc aaagctgaaa agcgaactgg acaatttaca gcggctggtt
     8761 gaccgtatcc cgccggatat actggcggag ctgaaacggc agcagcggca gcatggaaag
     8821 gaaaggtgat tttataagca gatttttacg ccgtccctgt gcggcattgt accttgaaaa
     8881 ttgaatacag gaggttctat ggagcatatc agatacaaaa aagaaacaga ggtcgtgact
     8941 tttcagggaa aagaaatcac gctggaaaat ctctccccgg tgttcacgcc ggatcaggaa
     9001 gcggcaaaac gccgggagct ggagcagcag ctttatgagg tgttccgcaa atatgccgat
     9061 aagcggcaga aagaggaagc cggggcgtaa ggttttccga agccatcgtt gatttacggg
     9121 gctgctggcg gtataataaa actgtcagca gctccgtttc tttttgaaga aaaggagcga
     9181 caatatgaac aacagaatag acgcaatcta tgcaagacaa tcggtagaca aaaaggacag
     9241 catttccatt gaaagccaga ttgaattttg caaatacgag ttgaaaggcg gtaactgcaa
     9301 ggaatacaca gacaaagggt acagcggcaa gaacacagac cgtccaaagt ttcaagaact
     9361 ggtgcgggac atcaagcggg gcttaattgc aaaggtcgtg gtttacaagc tcgaccgtat
     9421 cagccgttcc attctggact ttgccaatat gatggagctg ttccagcagt acaatgtgga
     9481 gtttgtgtcc tctacggaga agtttgacac ctccacgccg atggggcggg cgatgctgaa
     9541 tatctgtatc gtgttcgccc agcttgaacg ggaaacgata cagaagcggg tgacggacgc
     9601 ttattattcc cgcagccagc gagggtttaa gatgggtgga aaagcccctt atggcttcca
     9661 tacagagcca atcaagatgg acggtatcaa cacaaagaag ctggtggtaa acccagacga
     9721 agcggcaaat atccggctga tgttcgagat gtacgcccag cccacaacct cctacgggga
     9781 cattacccgg tactttgccg aacagggaat tttattcaac ggcaaggaac taatacgccc
     9841 cacgctggcg cagatgttac gcaatcctgt ctatgtgcag gcaaaccttg atgtgtacga
     9901 atttttcaaa agtcaaggga caatcattgt caatgacgct gccgatttta ccggcatgaa
     9961 tggctgctat ctgtatcaag ggcgggatgt gaagcccagc aaaaagaacg acttgaaaga
    10021 ccaaatgctg gtgctggctc cccatgaggg cattgtcccc tccgacatct ggctgacctg
    10081 ccgcaagaag ctgatgaaca acatgaaaat ccagtctgcc cgaaaagcca cccacacatg
    10141 gctggcggga aaaatcaagt gcgggaactg cgggtatgcc cttatgagta tcttcaatcc
    10201 ctccggcaag caataccttc gctgtacgaa acggctggat aacaagagtt gtccggggtg
    10261 cgggaaaatc atcacttcgg aactggaaac ggttgtttat cagcagatga taaagaaact
    10321 ggcaagctac aaaacgctga cgggcagaaa aaaagcggca aaggcaaacc cgaaaatcgc
    10381 cgccctgcaa gtggagcttg cccatgtgga cggcgagatt gaaaaactgg tggacagtct
    10441 gacgggcgca aacaatgtgc tgctctccta tgtgaatgtg aagatagcgg aactggacgg
    10501 acgcaagcag gaacttctgg cgaggatagc ggagttgacg gtggaagcca tcagcccgga
    10561 acaggtcagc cagatttccg gctacctcga cacttgggag gatgtatcct ttgatgacaa
    10621 gcggcgtgtg gtggatttga tgattaccac catagccgcc acaagcgaca gcttgaacat
    10681 cacatggaaa atctgacggg cggaacccct cccgtcagat acctaccctg tgtagtccct
    10741 tgtaaactgt actttacaaa agctttaaag tcaggtctta caattggtat aggatttgtg
    10801 ggtataaact tagttgtaaa cctattaaca tcaacactag ggccagcggc tcaaggaatg
    10861 gttgaaagat tcggacttaa tttaacagta atagatgttg gttggccaac aacagcatca
    10921 gcaacatggg cttcaccagt tgcaccaata ttaataccag tttgtttatt agtaaactta
    10981 ctattattat acttcaataa aacaaagata ttaaacgttg atatatggaa ttattggcac
    11041 tttatagtag caggtgcagt tggatatata gtaacaggaa gttatgtttg ggctatattc
    11101 tgtgcaatac taatggagtt attagtactg tttattgcag aaaagacagc aaaaaatgtt
    11161 caggagtttt atgatttaga aggtatagca ttaccaactg gttcaacagc ttcatttgct
    11221 ccacttggat atttagtagg taaatttgtt gaaaaaatac caggaataaa taaaatacaa
    11281 gcagacccag actctataca aaaaagattt ggtatatttg gagaaccaat gatgatgggg
    11341 attatactag gtataatact tggagcactt gcgggatatg atgtagcagg aatattcaat
    11401 gtaggtatat caatgggtgc agtaatgtta cttatgccta gaatggttag aatattaatg
    11461 gaaggtttaa ttccaatttc agaatcaata agagatttct tacaagcaaa atatggagac
    11521 agagaacttt atataggtct ggatgcagca gtagcaacgg gacatccagc agttatatca
    11581 acagctctta tacttgttcc tataacgttg ttcttagcag ttatactacc aggaaacaaa
    11641 gttttaccat ttggagactt agcgacaata ccattttata tggcatttat agtgtgcttt
    11701 agaaaaggta atatagttca atcagttata acaggaacaa tagtaatagc tatatcactt
    11761 tatatggcta caaactttgc agatgttcat acacaattat tacaacaagc aaatatgatg
    11821 ccagaaggaa caacaatggt tacaagtata gatacaggtg gaaacttgct aaaatgggga
    11881 atattgaaat tatctcaatt agtttcttca tttggaatat tctaatatgt agttaataga
    11941 gattactctt ttttagtaaa gtatgcgaaa atttaacatt tagcagctac taaaaaagag
    12001 taatattttt tataaatttt taaaaataca aaatacaaga ggaggagatt tatcgtgaaa
    12061 gcagcagtat tacatggcac aaatgatatg agatttgaag atatagaaat aaaaccatgt
    12121 gaaagtgatg aagtaaaaat aaaagttatg gcagcaggaa tatgtgggtc agatcctcca
    12181 agagtattaa aacattggaa atatccagtt ccagctatac caggacatga gttttctgga
    12241 gtaattgctg aagttggtaa ggatgtaaaa aatgtaaaag ttggagatag agtagttgca
    12301 attcctttta taccttgtaa tgaatgtgaa tattgtaaaa gaggattatt ttcattgtgt
    12361 gatgaccatg gaatgttagg agctaaaagt tttggagctt ttgcagagta tgtaaatata
    12421 aaagctacaa atgtacttcc aataggagat atggactttg aagatgctgc aatgatagag
    12481 ccactagcag ttgctatgca tggagtactt aatataggtg ttcaagttgg agacgctgta
    12541 gccgtaatgg gtagtggaac aatggggcag ttggtaatac aaggacttaa aatagcagga
    12601 gcaggaacaa ttatagcagt tgatatatct gataataaat taagagaatc aaaggaatta
    12661 ggggctgata taataatcaa tgctaaagat attgacccag tagaaaaaat aaaagaactt
    12721 actggtggaa aaggcgttga catagctctt gaatgtgcag gttcaaaaat aactcaagaa
    12781 caatgtttac ttataactaa aaagaaaagt aagataggat ttttagggat agcatattca
    12841 gatataactt tatcagaaga agcatttgaa aatatattta gaaaagaact agaattaaaa
    12901 ggtttttgga actcttattc tgcaccattt ccaggacaag aatggactaa gggaatcaat
    12961 ctagttaatg aaggaaagat aaaattaaaa gaaatggtat ctcatagatt ctcacttgaa
    13021 gatacttaca aagcatttga aatgataaga gatagaaaag aagaatttaa caaaatatta
    13081 attttaccac aaggggttga aaaataatga aagcagtagt taaaactaaa ccaggctatg
    13141 ataatgtaga agttttagaa gtagaagaac caaaagctac aggagacaag gttaaaataa
    13201 aagtagaata tagtggtata tgtggttcag atatacattc atttaaaggt gaatatgcaa
    13261 atataaaagc cccagtaaca ttaggtcatg aattttctgg aattgttgta gaagtaggag
    13321 aagatgttaa agatataaaa gtaggagata gagttacatc agaaactact tttgcaactt
    13381 gtgagaaatg tatatattgt gaaactaaag attataactt atgcccaact agaaaaggta
    13441 ttggtactca agtaaatgga agttttgctg agtatgtact atcaagagaa gaaagcatac
    13501 atgtacttcc tgaaaatgta tctttattat cagcagccct tacagagcca ctagcttgtt
    13561 gtgttcatgc agctcttgaa aagactacta tagaaagttc tgatacagtt ttaataatag
    13621 gaccagggcc aattggttta ttattagctc aagttgttaa atctcaaggt gctacagtta
    13681 taatgtctgg tgttacaaaa gataaggaaa gattagaaat agctaaaaat cttggagtag
    13741 atagaactgt aaatgtaatg gaagaaagtt tggcagatgt agtgaatgaa atgactgatg
    13801 gaaatggagt aaacaaagga tttgattgtt ctgggtttat gccagcagtt aacgaagcct
    13861 taagactgct tagaaaaaaa ggtactttcg tacaagttgg aatctttgct aaaaaattaa
    13921 atgaaatgga ccaagaagca ataatccaaa gagagttaca atatatagga agtagatctc
    13981 aaaagccaag ttcttggatt acagcattag atttaatggc aagtgggaaa gtaaatacag
    14041 aagctttagt tactagaact gttggtttag acgaatttaa agaaggtatt acagctttaa
    14101 tgaacgggga agaaataaaa gtagcagtaa aatcttaaaa aaacatttgt attattttaa
    14161 aaaaaattgt aaaatctact gtaggataaa aaaatgtaag gtcttaaaaa ataacaggag
    14221 gaagaaagta tgagcactct tagagatcat tctaataata ttagaaaaaa cataataaaa
    14281 atggttgcag aagctaaaag cggacatcca ggtggttcat tatcaatagc tgatatgttg
    14341 acagtattat attttgataa gatgaacgta tgtgcagaaa atccaaaaat ggctgataga
    14401 gatagatttg ttctatcaaa aggtcatgca gcaccagctt tatatgctac attagcagaa
    14461 aaaggatttt tcccagaaga agaattaata acactaagaa aatttggttc taagttacaa
    14521 ggtcacccag acatgaaaaa agtagcagga atcgatatgt ctactggttc tcttggtcaa
    14581 gggctatctg cagcaaatgg tatggcactt gcaggaaaat tagataacaa agactataca
    14641 gtatatacta tattaggtga tggagaaatc caagaaggtc aaatatggga agctgcaatg
    14701 acagcatcac attacaaatt agataattta attgcatttg ttgacttaaa tggattacaa
    14761 atagatggtt ctaatgaaga agtaatgaat gtaaatccag tagatgacaa attcaaatct
    14821 tttggatgga atgtaatagt aataaatgga cattcttttg aagaaatagg aaatgcagtt
    14881 gatgaagcta aaaaagttac aggaaaacca actgtaataa tagctaaaac agtaaaaggt
    14941 aaaggtgttt catttatgga aaataatgct gcatggcatg gaacagctcc aaatgcagaa
    15001 caagcagagc aagcattgaa agatttagaa ggaggtctat aggaatggga atagcaacta
    15061 gagaagctta cggacaagtg ttaaaagaac ttgcagaaaa taaagatata gtagtattag
    15121 atgcagattt aggaaaagct acaaaaagta tatcatttaa agaagttgcc ccagatagat
    15181 tttttgacat ggggatagca gaaggtgaca tgattggtac agcagcaggt cttgctactt
    15241 gtggaaaaat accttttgcg agtacttttg caatatttgc agcaggaaga ggctatgagc
    15301 aaataagaaa ctctgtagca tatccaaatt taaatgtaaa aatagcagct actcatgcag
    15361 gtgtaacagt aggtgaagat ggtggtagcc atcaagctat agaggatata tctctaatga
    15421 gaggtatacc aaatatggtt gtattaaatc cagcagatgc tttagaagca agacaagcta
    15481 ttcttgcatc tattgattat aatggacctg tttatataag attaggtaga gctgcaactc
    15541 ctgatgtaaa cagtgaaaat tataaatttg aaataggaaa aggaactgtt ttaagagaag
    15601 gttctgacat aacagtaata gctacaggaa taatggtagc aaaagcctta gaagcagcag
    15661 aagaacttgc taaagaaggt ataaatgtag aagttgttaa tatatctaca ataaaaccat
    15721 tagatgaagc attaataaaa gaaactgcta agaaaactgg aaaagtagtt acagcagagg
    15781 agcacagtat aataggtggt ttaggttcag ctgtatgtga agctttagca gaaactaaag
    15841 atgtagtagt tagaagaatt ggtgttaaag atgtatttgg tcaatcaggt acaccagctg
    15901 atttattaaa acactatggt ttaactacag aagatatagt aaaaaatata aaagagttgt
    15961 tatagatata aatatatcta aataacaaat tgatttgaca taagtcaatt aaagctgtct
    16021 taaaaataga aagaaatttc tacaatggga cagctttatt tttacaatac tttattttta
    16081 tgataattat aaaatagtct gaaagtaatc aaaaaaatta taaaaagagg tgctatttaa
    16141 tgaaaatatc tattggatgt gaccatggtg gttatgaatt aaaggaatat ataaaggaaa
    16201 atttaataaa taaaggtata gaagttgaag atgtgggtac aaattcaaaa gaaagtgtag
    16261 attttccaat atatgcaaaa aaggtaagtc aaaatgttca aagtaaaaaa tctgatttag
    16321 gcatactttg ttgtggaaca ggaataggca tgtctattgc agcaaataag tttaaaggaa
    16381 tcagagcagc tgttgtttct gactgttttt cagctaaggc aactagagaa cacaataata
    16441 caaatgtact ttgtttagga gaaagagtta ttgggaaagg attagcaatg agaattgttg
    16501 aagaatggtt aaattcaagt tataatggag aaagacatgc aagaagatta caaatgattg
    16561 atgatataga aaaagaaaat ttataaatat atataatata taatttataa caagggggag
    16621 cgttatgtct attatttgtg cttcaataat gtgtgcagac caaatgaagt taaaagagga
    16681 attagaagct cttgaagaag caggtgtaaa attacttcat tgtgatgtta tggatggaat
    16741 atttgttaaa aacttggcta tgggaccaga gttattaaaa tcaataagtg aaaatacaac
    16801 aataccttta gacatacact tagccacaga aactccagat aaatatattg atatgatgtc
    16861 ttatataaag ccaaaatata tttcttttca tgtagaatca agtacaaatg taaaagctga
    16921 tatacaaaag ttaagaaatt atggtatagg gccagtgtta gcaataagcc ctcaaacaag
    16981 tgtggataaa atagaagaat atataagttt agttgagggt atcttagtaa tgacagtaaa
    17041 tccagggttt gcaggtcaaa agtttaatct ttctgtattg gataagttgg ataaacttac
    17101 agagatacta aaagattatg ataatcctcc atttatagag gttgatggaa atataaataa
    17161 ggatactatt aaacttatga atggtaaaaa agtagatata tatgttgttg gtacttcagc
    17221 tttatttaat gataagcctc caatttcata taaagataaa atagaagaac taaaagaaag
    17281 tataaagtaa ccatgtaata aaattataat atgtttctgt ttaatagtaa aaatttaggt
    17341 ggaaacaaga aattttataa tatttaataa aaattttggt tgagtaagga gaatttttta
    17401 tgggagacct atataaacta caaaatggta ctgatattag aggaatagcg tatgaaaatg
    17461 attctaaaga agttaaccta actgtagaag aagtaaaaaa aatagctaaa gcgtttcata
    17521 tttggctaaa agaaaagact aagaaagata aagtaactgt tgccatagga actgattcta
    17581 ggataacagg aagccaattt agaagtactg ttattgaaac tttaactaat gatgattgta
    17641 atgtaattga ttgtgagatt gctacaactc cagctatgtt tatgacaact attatggatg
    17701 gatacaattc tgatggggcg attatgatta cagcaagtca cttaccttat tactataatg
    17761 gtttaaagtt tttcacagaa agtggaggtt tagaaaaaac taatataaaa gagatgttgg
    17821 atatagctgt taaaaatgat agtgtagact atgaggaagt aaataaaaaa ggtgaagtta
    17881 tagtaaaaaa tttaatagaa gattattcta atttattgat agataaaatt agaaaaggtg
    17941 taaattcaag taaaaattat gaaaagcctt tttctgggct taagatattg gttgatgcag
    18001 gaaatggagc tggtggattc tttgctgaaa aagttttaca catactggga gcagatacga
    18061 caggcagtca gtttttgaac ccagatggta tgttcccaaa tcatatacct aatcccgaaa
    18121 acaaagaagc aatggaaagc atatgtaaag ctgtgttgga taataatagc aacctaggaa
    18181 taatctttga tacagatgta gacagagctg ctattgtagg taaaaatggc aaaccaataa
    18241 ataaaaatgc tcttatagct gttatatcat caatagtatt agaagaacat ccaaaaacag
    18301 ctatagtaac agattcgatt acttctgaag gacttgctaa atttataaat gaattacaag
    18361 gaagacatca tagatttaaa agaggatata aaaatgtaat aaatgaagct ataagattga
    18421 atagtgaagg tgaagagtgc catttagcta tagaaacgtc tggacatgct gctttaaaag
    18481 aaaattattt cttagatgat ggggcatatt taatagctaa aattttaata aaagtagcaa
    18541 agctatcctt ggaaggaaag actatagaag aattgataga aaaccttgaa gaagctcaag
    18601 aagagaaaga agttagaata ggcattaaca aagaagattt tagaccatat gcagaggata
    18661 tattaaaaga tttagattct catgttaaga gtattgatgg atggtctgta gcaccaaata
    18721 attttgaagg aataagagtt aattgtgatg aatcaaatgg agatggatgg tttttactta
    18781 gaatatcact acatgaacca ttactagctt taaatattga atcaaataaa attggtggag
    18841 cagaacaaat ttattctaag ttaaaacttt tcttagaaaa atatgattta aaaggtcttt
    18901 agggtaaata gaaagaatat atttatatta aagaacaaat gtaaatttgt acctaagatt
    18961 acaaatatta caaaattgta atcttaggta acagagatgt aactgattat ggaaacacta
    19021 tgtgatatta tagatacaga gagatgaaat atgtgatgaa ttatagagaa atataattta
    19081 aaatgtatta atatcacacc aaaaaggtat aatcaaaaaa tatgtaaata taaaccccaa
    19141 catttagaaa aagacctcta aggaggtttt tttcttgttt aaataactta aaatatattt
    19201 ttatattaat atatcaatta actcttctaa aagtaagcat taccttaaat aaatcagcat
    19261 ctataattat ttccatgtgt gcattttcta attcaattat attttgagca attgataaac
    19321 caagaccaga gccatctgtt tttctagata tatcacccct tctaaatctt tctattaact
    19381 cttctggctt tagatttagt ttatcatttg aaatattttt aaacgttaat attatatttt
    19441 cttcctttat aaacatatct atatagaccc tagtgttagg catagagtat tttagtatat
    19501 tgcaaattag attctcaaaa actctccaag tttttttacc atctatcatg gtaaaaacag
    19561 catattcagg tgtttctatt ataaaatcta aactgctatt ttgaattttg tcttcaaact
    19621 ctacaatact ttgaattaaa agttctctta tatcggtttt tgttatatta aattctacat
    19681 ctccactact tagcttagat gcttcaaata tgtcatctat taataatttt aatctttgtg
    19741 attttttatc taaaatattt atatagtcat ttatagtctc tctgtcaaag tcattatttt
    19801 ttaacatatc aacatagctt aatatagatg taagtggagt ttttaagtca tgagatacgt
    19861 ttgtgataag ctctgccttc atcctttcat tttttatatt agcatctaca gcagtttcaa
    19921 gaccacctgt tatattattt atacttttgg ctaaagtaga tatttcatca tctcctataa
    19981 cttctaattt atactttaaa ttgccatttt ctatattttt tatatttttt gatatgttat
    20041 ttatgtaatt aaacttaaca gataaaaagt tgtatatata aaaatgaaaa actgtgttta
    20101 caagtgtaaa taaaattact atagctattg cctcagaata atatatatac ataaagaata
    20161 tcaaaacaag atattggata atagcttgta caactataga tattaaaaat actatagcta
    20221 attttttatt tatagttttt ttattataca tactaagtaa gtttgaaaag aatacatatg
    20281 ttatagacat atcttttaga ttatagttat tatatttaat attttcatat attatataga
    20341 ctatagatat aattataaaa ttgtagatta ttaaattagt gttgtaaaac attttattac
    20401 ttgtaaatgc atgaaacctt aaggttaata gtatcaaagt taatataagt gtaagttcta
    20461 tttttacctt agaaaaaagg cttatgacat tctctaagaa tttgacttta gtctgtttat
    20521 tttttttata caatgtaaaa catagtatag tcaataaaaa acttgataat ataataccta
    20581 tgcagacaaa ctctaattct acttttgaca aatgtacagg agtaccgaaa aaataaaaat
    20641 aagggagagc aaactcttta taataacaat taatattata agaaaagtta ttattcaaat
    20701 cataaactgc ttcaaataat atagaaacta atagaaacag taataataat aaaataaatt
    20761 ttgctttaga atcattaata attaagtttt tcaattttgt aaccaatgcc ccacaccacc
    20821 tttaaatatt ttggttcttt tggattaatt tcaatttttt ctctaattct tctaatatga
    20881 acagcaatag ttctctcaca actataagca tcttcattcc agacattttc ataaatttca
    20941 cgggaagaga aaattttacc ttttcgtgac ataagaagtg taagtatttt atattcaagt
    21001 ggtgtcattt taatttcctc accatctaaa gaaactattt ttgtatctgt atttagctct
    21061 aggccaccac tttttagaat ataattagta tcatttgagt tatttgtatt actaaaatca
    21121 cctaaattta tataccttct aagttgagat tttactcgag cagtaagttc tagtggatta
    21181 aatggtttag ttatatagtc atcagcaccc atattaagac ctataatttt gtccatatct
    21241 tctgattttg cagataagag aattattggt atattccttt tttctcttat tttaagtgta
    21301 gttttaagcc catctaaatt gggcatcatt acatccatta ttattaactt aatatcattt
    21361 tcgattagta tgtccaatgc ttcaagtcca tcatatgcct ttaaaacatt aaagttatca
    21421 ggtctcaaat agaactctat agcatcaact atctctctat catcatctac aaccaatata
    21481 ttttgattca atttattaac ccccttgaaa ttttattttt aaccaatttt tattttatac
    21541 caaaaagagg tattagaaaa cagtaagccc gttcattatg aaataaaaac tagtactttt
    21601 aatatattgt caatgcatat agtaaattta aaagtataga cgcccatatt aatctgaagg
    21661 taaaagttta tgaaaatatc tagggtaatc tatcacactc cttatgttat tttgggttta
    21721 ttaatttttt aggtttaaac atatattaac aaatataaat aacaaatgag tttcataatt
    21781 attaagaatt tataacaaat atttatttta atgtagttta atataaatag gtagtttaat
    21841 gtagatatgt ataaagtatt taataaggct tataggtttt aatgatgata tctttatata
    21901 ttttaagaga tgatgaaatt agatatgggt aatagttgaa tgagaaaaag tagtatagca
    21961 caaaaagtgc tatatcacaa aatctcaaat aatttccttc ttataactta aattaaaaga
    22021 ataaatattt cttataaatt aggtgtatga agtatagcag gaacatcttt attatttaat
    22081 aacttatcaa atgatttatg gtcagactta tagccaatac cttctctata ttctaattca
    22141 tcaacaattt tgtattgttg acaggcattt ttaacctttt catagtcttc agtagaatga
    22201 gctttataaa ctatcttatt attaaaagtt attttatcag caaatttagg ttttacaaaa
    22261 tatttagcat cttttatttt accaacatta tataaatttt ggataacaga accatttttt
    22321 aaatgttgta aatggtcatc taacttataa gtatgtgcta tatgagttac atttttaaat
    22381 ttttcttcaa atgtaagagc aattttttcc atgtaattga agtcagtatt accatttgta
    22441 tttgtttctg gtaataatat tatattttcc cttattacac caaggctatt aagagaagct
    22501 aaaaattctc tatttctata ttctgatttt tgtatatttg ttaggttttc atatttttta
    22561 tatttatcaa agacggatat accacatcca gtagatacta aaactacaaa tacatgattt
    22621 tttcctcttt cttttatagc attaacaata gcactcccag cccacaacac ttcatcatct
    22681 tgatgctgaa catagaagac aacataatct ttaaattttt tatgactata attcacatag
    22741 ctactaaaac cggttaaaat caagcataaa actagaataa tagatagaat ttcatattta
    22801 tttatagtaa attttttatt cttaaatgtc atttaatcat ctcctgaaaa gttgtcttag
    22861 ttatattatc aaacaaagta ctaaaaatta tttcttaaat gctgaaaaat atagagtaga
    22921 ttaatggaat aaaatttttt ttattagttt aaaataaaga aaacatttat gtataagact
    22981 aatcaaaata ttaataattg ataggaattg agatttaaag ggattgtaca tttattcggg
    23041 attcatttta atagaatttt actttagtac ttagaataaa agtaataact ttttaaattt
    23101 aaagagtaca catatatatt tgtgtactcc ttgatgatat gattgcttat ttactacaaa
    23161 aacagagtct ttttaaacct aaaatatttg atgcaccagc tatcgtagtt ccataatagt
    23221 aaactggttt gttaagtgat agaaaattta taattgagtt attacataat gtacttcctg
    23281 tgcaaatcac taaatcagcc caagatatta catcttcata atctcgtata ccatcttcaa
    23341 tcttaacatt atattgaata gtatccacaa attcaggatt taaatctaat actcttgttt
    23401 cgaaaaagtc cttaatatta tcaattatgg ctggttgata tcctataatt gctacttttg
    23461 gattaccaaa ttccattttt ataaactcag ggaatttttt tgcacatacc tctggctcgt
    23521 tatttttaca atgaatggtt ctatccgtct tgcctaagta tttcataact gcatttaatg
    23581 atgctataaa aagacttcta tcatgaggat tgtcatgtaa atctaatgat aatatgtctt
    23641 ttaaagaacc ttcaaatgta gatggagtag aagtaaatgc ttgtccttta gctcctttaa
    23701 agtcagcttc aagcattatt tctttaccaa ctataattgg gaaatcttta cgcttagtat
    23761 ttccaattgc ttcttttgaa gatagagatt tggacacaat acttatcata tccatattta
    23821 aattatattt atcgacttgt actaatagat aatctctcaa aatatcaaat atattcatag
    23881 aaaaaagatt ctcctttatt ttttatttct tcatttaaat caaaaatatg atttattttt
    23941 ccattgttca ttattcctat cttatgagca aaaaacattg cttcagaaat atcatgagta
    24001 acatgaatca ctgttggttt gtattttatg tagacatcct tcaaaatttt gcttatatgt
    24061 ttttttgtaa tataatccaa tgaactcata ggttcatcta aaagtaatat atcaggttca
    24121 gctattaagg cacgtgcaaa agcaactcgt tgcttttcgc ctccacttaa atttttagga
    24181 tacctatttt ttatatcttc tatctgcatt aatttagaca agtggttagc cttaagaatc
    24241 tgttcatcta taggtatttt tcttaattta agtggaaacc ttatattatc ttcaacattc
    24301 atatttggaa ataattcaaa attttgatat acaaatccta tatttctatt ttctggaggt
    24361 aagtttgata ttaaattttc tttgtatgat acatttcctt tagcagatgg atatcttcct
    24421 gctaatgttt ctaggaatag agttttacct gacccactag ctcctaataa aacaaagtat
    24481 tcatgattat taatatttaa actattttct tgtaatttaa aattaggtaa atcaagagat
    24541 aaatttttta tttttaacat attaattctt atcaacctct ctattaaaaa atatctcaaa
    24601 aagcatcaag catgtaagtg agataaatat caaaattaca gctattccga ttgcaatgtc
    24661 tatatcacca gtagacatat ttaaatatat agatgtagga ataatttcag ttttcatacg
    24721 tgtagaacct gctatcatag ctactgcacc aaattcacca agtgctcttg accaagtcat
    24781 tacagttgca gatatgattc cagtttttag atttggaaga attataaatc taaatgcttc
    24841 ccaactatta catccaaggg tacgtgcaac aaatatcatc ttttcattac tttcatttag
    24901 tgaagtgtga agtattttaa tagtaagtgg aagatttaca aatacttgtg ctaaaactat
    24961 accttgcttt gtaaatataa aatcaagctt aaaaatctta taaatggaat ctccaatccc
    25021 cattcttcca aacaaaagta aaagagcaat tcctgatact aagtgtggaa gagacatagg
    25081 gagatttatt atctgtataa tcagtttttt aaatggtaat ttagtaatat gtaaaaaata
    25141 tgctactgga atagctaata aaaggcatac tatagtagat attaaagatg ttttaatact
    25201 aagtgttatt gcatactgta cttccacgga ttttaagcat ataggaacat aacttatccc
    25261 tttaatgatt aacataagaa gtggtgatac aataaaaaaa aagactatag caaaaaatcc
    25321 agtaagtagt gtagtaaaaa atttattttt caagtttaat cactacttaa caggttcaaa
    25381 accatatttt ttaaatatct gtttagcttc atctgactct atataattta taaatttatt
    25441 agctaaatct tttttatcac tatagtttac gattcctgct gatatgagtt ggtctatatt
    25501 tttatcttgg gcaatttcta taacttctac atcttcattg ccaaagaccg aatcttttgt
    25561 ggctattgta gcatcagctt tacttgatgt taactgtaca atcatttcat ttacagttgc
    25621 agctgtagag acaacattat catttataga ttttaaatta tttttttcaa gtattttttg
    25681 tgtagtctta cctatagcat tagactcttt gtctcccaaa ataagtttaa ctccatcttt
    25741 atttaagtct tccaattttt gaatattagc tggatttcct ttaggtacaa ctattgcagg
    25801 tgtatgatgt gcaaccgttt tatgttcacc tacaagttct ttttcaaagg cctttgaata
    25861 aattggttca gaaccaacaa taaatacgtc tcctttgtga cttgtctcaa tttgagctat
    25921 tagctgtgca gaacctgcat agctatattc aactttgaca ttattttctt tctcaaattt
    25981 ttttgctatt tcatccattg gtttctttaa acctgcccct gaatagacaa aaagagtctc
    26041 tttttctttt gaatcatcag ctgttgtcac tttagtatta gattcatcat tagctttgtt
    26101 ttctttagaa ttacatgcag taatacttaa tgaaataatc atagctaaag ctagtactga
    26161 aagtttttta aacataagat attccccctt agtaaattag tgtaaattct tttttagtat
    26221 atcatactaa gaaaacgatg tctaagtttt tgttatgttt tataatgttt tgttatgttt
    26281 tattatgtat agttaagaaa aaatctattt ataatgtcat ttccataggt agttatttta
    26341 tttttaattt ttattaatac tttaaagtat tttaaa
//
LOCUS       DAMKPG010000050        26249 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMKPG010000050 DAMKPG010000000
VERSION     DAMKPG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.053"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     repeat_region   535..891
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=535..563
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1130..1318
                     /locus_tag="PC621_002171"
     CDS             1130..1318
                     /locus_tag="PC621_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109323.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1437..2303
                     /locus_tag="PC621_002172"
     CDS             1437..2303
                     /locus_tag="PC621_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HDF4109324.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3168..3695
                     /locus_tag="PC621_002173"
     CDS             3168..3695
                     /locus_tag="PC621_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF4109325.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3833..4600
                     /locus_tag="PC621_002174"
     CDS             3833..4600
                     /locus_tag="PC621_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HDF4109326.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4666..7038
                     /locus_tag="PC621_002175"
     CDS             4666..7038
                     /locus_tag="PC621_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF4109327.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7055..7744
                     /locus_tag="PC621_002176"
     CDS             7055..7744
                     /locus_tag="PC621_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF4109328.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7737..9629
                     /locus_tag="PC621_002177"
     CDS             7737..9629
                     /locus_tag="PC621_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF4109329.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9643..9903
                     /locus_tag="PC621_002178"
     CDS             9643..9903
                     /locus_tag="PC621_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF4109330.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            9908..10327
                     /locus_tag="PC621_002179"
     CDS             9908..10327
                     /locus_tag="PC621_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF4109331.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10328..11377
                     /locus_tag="PC621_002180"
     CDS             10328..11377
                     /locus_tag="PC621_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF4109332.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11370..11987
                     /locus_tag="PC621_002181"
     CDS             11370..11987
                     /locus_tag="PC621_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF4109333.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            11999..13024
                     /locus_tag="PC621_002182"
     CDS             11999..13024
                     /locus_tag="PC621_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF4109334.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13041..14567
                     /locus_tag="PC621_002183"
     CDS             13041..14567
                     /locus_tag="PC621_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109335.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14582..14875
                     /locus_tag="PC621_002184"
     CDS             14582..14875
                     /locus_tag="PC621_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109336.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14875..15057
                     /locus_tag="PC621_002185"
     CDS             14875..15057
                     /locus_tag="PC621_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109337.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15091..15657
                     /locus_tag="PC621_002186"
     CDS             15091..15657
                     /locus_tag="PC621_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109338.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15688..15978
                     /locus_tag="PC621_002187"
     CDS             15688..15978
                     /locus_tag="PC621_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109339.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            15982..16239
                     /locus_tag="PC621_002188"
     CDS             15982..16239
                     /locus_tag="PC621_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF4109340.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16500..17363
                     /locus_tag="PC621_002189"
     CDS             16500..17363
                     /locus_tag="PC621_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDF4109341.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17442..18254
                     /locus_tag="PC621_002190"
     CDS             17442..18254
                     /locus_tag="PC621_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4109342.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18348..20747
                     /locus_tag="PC621_002191"
     CDS             18348..20747
                     /locus_tag="PC621_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF4109343.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLLCGKNSLTKIDISKNKELNSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWENLSDFPNSISYSLEYEYGPVNDTET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFSGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(20788..20973)
                     /locus_tag="PC621_002192"
     CDS             complement(20788..20973)
                     /locus_tag="PC621_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF4109344.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            21668..22345
                     /locus_tag="PC621_002193"
     CDS             21668..22345
                     /locus_tag="PC621_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF4109345.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            22519..24555
                     /locus_tag="PC621_002194"
     CDS             22519..24555
                     /locus_tag="PC621_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF4109346.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(24746..25312)
                     /locus_tag="PC621_002195"
     CDS             complement(24746..25312)
                     /locus_tag="PC621_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4109347.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            25596..26123
                     /locus_tag="PC621_002196"
                     /pseudo
     CDS             25596..26123
                     /locus_tag="PC621_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT        10589 a         2868 c         4623 g         8169 t
ORIGIN      
        1 tgtttatata aaaaaaatgg aaaaatatgt aataattata ggttataata attgtagcaa
       61 ggataataat cgaaagtgcg aagggtgatt attttcatat taaacgccaa attccaaata
      121 aggaaggagg tgaaattata tgataggttt tttattaagc atactagctg gtgttatatc
      181 agcttatatt tatgacaaaa taaaaaatca cccagacgcc aataagggtg atttaaaaaa
      241 ataatatttt cacttaacaa ctgaaaataa tcactctttg taggaataaa ttatttcctt
      301 gcttttatta taccacaaat tggtacagat attcaaaaat aatattttta tgatataata
      361 aaaatgtaga gattttgcag tgagcgatat ttgtgataaa ttgaagttta acagttgcaa
      421 tataaggcgt tgagggtgtg tgataaatgt tatcaattgc actactcatg gttcactgca
      481 aatttaagag agttgtatat gtgtaggtat tgaaaatact caacttattt tggggtttta
      541 gattaactat atggaatgta aatgttgtaa gaagtatcat tctatttttt aatctttctg
      601 ttttagatta actatatgga atgtaaattt cagtgagaat aagctttatt gtcgatgtaa
      661 cactcgtttt agattaacta tatggaatgt aaatagtaca tataatgagt ctttaacatc
      721 agttatgaaa ggttttagat taactatatg gaatgtaaat gattgtactt tagcgtctgc
      781 actagctttg tctatcgttt tagattaact atatggaatg taaattattt tacagatgaa
      841 caattacagt tacttcttga atgttttata ttaactatgt ggattcaaaa ttaaatagaa
      901 aaaagaaagc acttacttaa atagtaggtg cttttgtttt gctcaaatta cttaattata
      961 aagttgctaa gtacaacttt ttagaattat acaatataaa aatgcactaa agtgattgat
     1021 tttgctcaag ttggtcggtt gagtaaaata attagaaaaa aatgttaaaa aactcttgaa
     1081 aagtgtcgcg atacaatgta taattatatt atcgcgatac agaaaagagg tgaaaattat
     1141 tactgatagc agtagagcag attacttcaa gcagagacga cagaataaaa aaacttttag
     1201 tgttctacta gatagagaga aagtagagaa aattgaagaa catttaaaaa agcagaacaa
     1261 gactaaaact atttggcttg aagaaaagat taatgaagag ctagaaaaag aggaataaaa
     1321 aataagagac gttctccccg accaaagatt gaacatccct tattgacgta tattatatac
     1381 actaactata gtatacgtca ttccttgaaa aaattcaatt aaggagtgta atagttatga
     1441 aaaatttaat agtaaaagag ttcaatggaa gtcaaattta tacttttatg tggaaagaaa
     1501 aatcttgttg gatagctaat caaatagttg gattattcga ttatgctgat gtatctaaaa
     1561 caatacagga ttgcataaaa gcagaagact ttgagattga acaagagtat gatgtattga
     1621 aaggaaatga atttaatgat tttgtaacta ctttaaatgt agtcgcaaat aatataatta
     1681 gtaataaagc tagaagtata actatttttt atgaagatgg tttatatgga tttttacaat
     1741 acacagacaa acctattggt gtacagttta ggaaatggct tagacgagaa gttttaccaa
     1801 gcataagaca aactggtgca tacataacta acaatgctaa tcccgaaaaa ctaagagaaa
     1861 aagcaagtga gattgagaaa ttacagttag cttacaatag tacatctatg ttaaaagaac
     1921 tattagatgg tgcaggcttt gacaataaat ccaaactatt aacagctaaa acattatata
     1981 agaaagcagg aattgattta ccaatagaga taaacgaaga agaacattat tttgatacaa
     2041 agcaaatagc atctaaactg aaaatatatt ctaagagtaa taaaccagca cagttggctg
     2101 tttgtgaaat tattaaaaag attgatttag aagaaaacga agtcaaaggc gtttgggaaa
     2161 ctaatggttc ttggactggt actgtaaata aatatacaaa gagtgtaata gataaggtta
     2221 gaaattggat agaggaaaat aatagaccta ctaagattgc aggtgaaaag aagaattatc
     2281 atgtggttta taagattgag taaatttata agattgtatt aaataattta gtttagtttt
     2341 ggggggatta atacaatgca tgagaattta cttgatagta tagatattga aaagagaaaa
     2401 gaagaattta gaattaaact tttgaaaata agagaaacag atatagatat atataataag
     2461 atagaaagta tagtatataa actttcttag aaaaaattag agaaaaataa ttaaataaat
     2521 agataaagca cttggatatt tcactgtttc aagtgcttta tatggtaaaa aatggtataa
     2581 taaaatatag gaattttgca gtgttcgatt tttctactca agtatagttt aacaattgga
     2641 atacaaggca ttgagaaggt ttgataagtg ttatcaattg cactattacc cgctcactgc
     2701 aaatttgaga aagttatatg tgtgtaagtc ttggaaatac acaatttatt ttggggtttt
     2761 atattaacta tatggaatgt aaatctttgt aatggtagtg tatttaagat tgaaacatca
     2821 agttttatat taactatatg gaatgtaaat caacattagt agttgtcttt atacacatag
     2881 catcacgttt tatattaact atatggaatg taaatacagc tcccaagaca tacaacgaat
     2941 ctgtaacatc agtgttttat attaactatg tggtatgtaa aagacttatt tacagcttta
     3001 tttgctaaat cagaaggttt tatattaact atgtggactt aaaattaaaa ataattaaaa
     3061 aacacttact aatgtaggtg tttttttatt gaaaggaagt gattataatg taaaaatttt
     3121 gaagatatag tataatattc ttataaaata taatttaggg ggatattatg agagaaggaa
     3181 agagaagaag aggatgtcta ttttggttta ttcttattat tttattttct ggagttgttg
     3241 gagcaatagc agggaacagt actaataatg aaagcactga aaaacagaaa gaggatttaa
     3301 ctaaatatat tggtgaagaa ggtagtatag gagatttaaa attaactgtt aatagcattt
     3361 caaaagctag tgaaatatca gtagcatctg gttatttagc atacactcca gatagtggca
     3421 aatatggtat tataaattta acaataaaaa atcaaactaa ggaaagccaa tcttttatgc
     3481 taaattcatt cacattaata ggaccagatg attcaaaata tgttccatct ttattgattg
     3541 atgtaggaaa taaatatata actatggata ctgtaaatcc taatttagat gtaacaggta
     3601 atattgcatt tgaaattcct aaggatttac tagtttctga ttgtaaacta aaatatagtg
     3661 gttcagagca agaatttata ttaaaagaaa aataatatat agatttaaaa atatgaagca
     3721 tttactttaa ataagtaagt gcttttttaa ttaaaaggag gtgataataa tgtaaaaatt
     3781 ttacttatat agtataatta cattataaaa ttattgtact ggggggaatc ttatggggtt
     3841 atttggaaag aaaaaagcag atatatgttg tatttgtaat actgagatag gagtactgaa
     3901 tattgaagat ggttggatat gcaattcttg ttttaaagaa tattgtgatg cactttctat
     3961 gactaaagca cctaaaattt taagaaaatt agatattgaa aagactatat catcaactaa
     4021 aaaaaataat gaacttcaaa aaatatttaa tacaactaat aatatagaaa actatataga
     4081 atttgatgaa gataataaaa aatggcttgt gtctaaaaaa agtataaacg ataaaaaaac
     4141 tcctatcatt cattcttatg aagatatcgt gaaatttgaa cttcttgaaa atggtgaaac
     4201 tgtaactaaa ggaggaatag gaagagcttt agcgggagga attttatttg gagaagcagg
     4261 agctatagtt ggagggataa caggtaaaaa aacaacaaga aaagttgttg atacatttaa
     4321 aattaaaata acaattaata atattgataa tcctattgag tatatcgaat taattaataa
     4381 aaaaacaaaa actaattcta gtgcttatga gaaagcttac aaagatgccc ataaaatttt
     4441 atcaacatta tctgctatta cacaaagcat taaagagaca gacaatataa atactaaatc
     4501 tgtagcagat gagatattaa aatataagaa tcttttggat atggaagcta ttacacaaga
     4561 tgaatttaat actaagaaaa aagaattatt gaatttgtaa tataataaac acttactaat
     4621 gtaggtgttt ttttatatgg aaatttatga aaggagagtg agaaagtggc aacgatacaa
     4681 acttcaataa agattttcga cggaatgaca cctgctttta ggaatatgac tacatctatt
     4741 aatacgacaa ttaatagcct tgaaaggtta caacaaagat tgcataatcc gcttaacaca
     4801 ggtgggatac aagcatctca acaaagttta aacaatattg aaaacatatt aaccaggata
     4861 gaacagaaaa ttggaagaaa tacaaatgaa caagaaaact ttaataataa gataaagcaa
     4921 ggaagtgaag caggttctct attagtatct aaactcaaaa gtttggctgg catatatatt
     4981 ggaataagag gtatagaaag tattacaaaa gcagcagata caattgcaag tacaaaagca
     5041 cgtttaaatt taatgaatga tggattacag acaacagaac aacttaataa gatgatttat
     5101 ttatctgctc aaagtgcaag agctagttat gcagatacgg cagcacaagt cgctaaactt
     5161 ggaatattag caggagatgc ttttggaagt tcggcagagg tgataaagtt tacagaactt
     5221 atgaataaag cttttgtaat tggaggaaca tcagcaaatg aagctagtgc ggctatgtat
     5281 cagcttactc aagctatggg tgcagggaaa cttcaaggtg atgaatttcg ctctataatg
     5341 gaaaatgcac ctttattagc cactaaaata gctgatgcaa tgggaaaaac taaagaccaa
     5401 ttaaaggaat tgtcaagtag tggagcaata acagcagatg ttataagaaa tgcactattt
     5461 aaagcttctg atgagataga aaagaaattc gcaagtatgc cagtcacttt tagtcaagca
     5521 cttactatga tgaaaaatga cgcctacatg atttttgggc aaaccctcgg aaagataagt
     5581 ggagcattgc aaagcgttag atttagtgaa attgttgtat ctataagaaa tgttatgatt
     5641 gcaatatctt caaacattta cgatacatta aatattataa aaaatatttt aaatagtgaa
     5701 ttcttttcaa acttagtgca aggatttaca ttttgtgcgg tagtaataac taaaagctta
     5761 ggttctattg taaatactgc attaaatata gttaatatat ttgcacaaaa ttggagtata
     5821 ataaaaccta ttgtatttgg tgtagcttct gtttttatat tgtttagagg ggttttgcta
     5881 gcgactaaaa tagctacaat aggtaacgta attgcgaatg ttgctcacgc tgcttcctct
     5941 tcactatcag cacttatgac taatatacaa gcagcagcac tgatgcgttc gaatggagct
     6001 actttatcag caacaatagc cacttggggt ttaaatgcag ctttattagc ttgtccaatc
     6061 acatgggtag tattaggtat tctagcattt gtagtagtag tttttgttgc agtagcagca
     6121 gtaaacaaat tcgcaggtac aagtttaact gccttaggag taattgtggg tgctgtattt
     6181 gcagcagtgg cagcaataca aaatgttatg atttggttat taaatggctg tatagctgta
     6241 aatgaaggca ttacaaatgg ttggaatcaa tgtgtatatc taatgaaaca agctattgca
     6301 aaaggtgtaa tctttataat cgagaaaatg gcatctctaa ataattctgt aaataatgct
     6361 ggaaatgcac ttgggaaagc tttcatagat ggggcaaata tcgcaatacg aggtgtaaac
     6421 aaattaattg acctaataaa taaaatacca gggataaata ttggtaaagt aggagaggca
     6481 acgtttacgc cagttaaggc agataatagt tacatcaaac aacagattga tagcttaaac
     6541 agatgggtag gagacgcacc agagaaagta aaattggagc gaatgggata caaagatatt
     6601 ggagcagcat ttcaaaaagg aaatgcactt ggaactaaat ggcaaaatgc tataactgat
     6661 aaatttaaag atacttttga cattaataag atgctagaag atgcaaagaa aaaattagga
     6721 ttagacgatt tgtggaataa acaaaatcct ttaaacaacc ttggtggatt tggtggagat
     6781 ttaggaaaaa atgtaaagga cacggcaggc aatactgcaa agatggcaaa gactatggat
     6841 aaaagtcaag aggacttaaa atacttaaga gatatagcag agcaagaaac aatcaatcga
     6901 ttcacaggag taaacattaa aatagatatg aacaatacaa acaacataag taaagataca
     6961 gatgttgatg gaatagttaa tgtcttaact gaaaaactga acgatgctat ggttgtatca
     7021 gcggaaggaa tagtttagga aggagagtga gaaaatggct tatgattttt acctagatgg
     7081 agtacaacta ccaatcgcac cacccaagct tgaagtcaaa gtgacaaaca agaacaagac
     7141 agttgatttg ataaatactg gagaagtaaa catattaaaa aaagaaggat tatctgaaat
     7201 aagttttgaa gcagaattta cacataataa actacctttt tgtagaggtc aatttagaga
     7261 tgttcaattc tttttaagta agctagaatt actaaaaact gattgtaagc catttcaatt
     7321 tattgtatct cgtgagttag gtaacaaggt cttatttaac actaatataa aagtatctct
     7381 tgaagagtat gctatttcag aagatgcaga taatggctca gatacaaaag ttgcaataaa
     7441 attaaagcaa tatagagatt actcaactaa aaagttagtt cttgcccctc ctaaaaatga
     7501 gactggtaga cctaatgtaa agatagagcc aaaacgagtt gattcagtca atgccacaaa
     7561 cactaaaaca tatacagtaa aagcagggga tagcctttgg tcaatttgtc agaaacaact
     7621 tggtaatggt tcattatata agaaagtata cgaactaaat aaatctatga tggataaggc
     7681 aaataagggc aaaaacttaa gtaaatacac tatttataaa gggcaggtgt taaaacttgg
     7741 ttgatgaatt agtgttagca aatgatagag atgtaagatt agtaatagct cattgggaag
     7801 atttctacga acctgctgtc attgatggta tcacatggga gatagaaaga cgaggtacac
     7861 cttctaagtt agagtttaca atagtcatgg atgatatact agagttttgc gaaggaaatt
     7921 ctgtaaggct gtattataaa ggaataggca tattttatgg atatatattt caaaagaaaa
     7981 gagataaaga aaatcacatt aaaattgttg cttacgacca gttgagatat tttaagaata
     8041 aagatactta tgtatatagt aataaaactg caagtgaact tgtaaaaatc ttggctaaag
     8101 attttaattt aaaatacaat gtcatagaag atactaagta taaaatatct agagtcgaag
     8161 aaaataaaac actctttgac atgatactaa cagcactaga tgatacttta agagagaaaa
     8221 aagaaatgta taccttgtat gatgattttg gaagaataac attaaagaat gttgcatcaa
     8281 tgaaactaga tactgtcatg aacaatgatg taattgagga ttttgactat aattcatcaa
     8341 tagatagtga tacttacaca aagattaaac ttgtaagaga caacgaggag tcaggaaaaa
     8401 gggatgtata tattgctcaa gactcagctc acatgagaag ttggggaata cttcaaatgt
     8461 ttgatacagt agacaaaaac atgagtgaag cagagataaa acaaaagtgt gatatacttc
     8521 taaaactata taataagaaa actaagtcat taagtttaaa aaatgcactt ggagatatta
     8581 gagtgagagc aggttgttta gtacctgttt ttttaaatct aggagatatt gaattgcaaa
     8641 attatatgtt agttgagaaa gtaaaacata catttgaaaa taattcacat ttcatggatt
     8701 tgaccttggt tgatggagac gaatttgctt catattcttc aagctcatat agtagtggaa
     8761 atactaataa taaagatgaa aagaaaaatg gtcctgcaca aagtattatg aaaaaaaata
     8821 caggtaaaaa agttcctgct atatttactg catattatcc aggaaacaat gcaatggaag
     8881 gtggaaaaac agattgcaat ggaaagccac ttgatgtaaa atcaagaact gttgctggtc
     8941 caatgaatcg agaaggagtt aagaaaactt ggtatactga tgattttcta aataaacatc
     9001 cagtttttga atatggagat aaagtaaaaa ttatacttcc tggtactgcc tatgacaaca
     9061 aagtatatac agttaaagat aatggaggaa gaatatatgt tgaaacaaac ggaacatatc
     9121 atatagatat actattagct aatgctagtg aatgtaaaaa atttggtaga aagaatggct
     9181 atataattat aggtggagat gaagaacaaa catatcaagt tgaaggtaat aaccaaagta
     9241 atacaaataa caattctaaa gaagataaat taattagtat agcaaaaagt aaactgggtt
     9301 gtaattatgt gtatggagca gaaggtccta ataattttga ttgcagtggg tttactcaat
     9361 ggtgttataa acaaataggt ataaaaattc ctcgtactgc ttctgcacaa agtaaagcag
     9421 gaaaagcagt agatttaaaa gatagaagca agtggaaagc aggagactta ttatgtagaa
     9481 ttggtggagg aagtagtaat catgttgtaa tgtacattgg aaacaatcaa ataattcatt
     9541 caccacaaac aggagatgtg gtaaaaatag agtctgttaa ttcttataga aaaggaaaag
     9601 catacacaca tgtgagaaga tttatataag tgaggtggca atatgagcca agatttatta
     9661 cagataataa aaaaagctgc aatggatgca gtagaaacaa gcaacccaat gagggttgta
     9721 tttggaacaa tagaaagtat tagtcctcta agagttaaga tagaacaaaa actatctatt
     9781 ggtgaaattt ttctaataca aacagataca tttaaaagat atacagataa aaaaatggga
     9841 gataaattag tcttaattcg tatgcaagga gggcaacaat acttgatttt agataggatg
     9901 tgataaagtg ttaccaagtg ataatttaga ttatgatatt gaagatgtat caataattaa
     9961 ttttgatgta agacaagagc caagtaagac atttaaactt catatagaaa agtctaagat
    10021 tgatggtatt tgtgatgatg tagaagcatt aaaacaaacc atttttttaa ttttaaatac
    10081 tgaaaggtat gagcatctta tttattctag aaattatggt gttgaattaa atgatttaat
    10141 tggagaacct atttcatatg taatacccga acttgaaaga aggataacag aagcactaat
    10201 tcaagatgat aggattgaaa atatagataa ttttgagttt caaaatataa agggtaaagt
    10261 acaatgtaga ttttcagttc atacaaaata tggaaatata aaagcagaga aggtggtgag
    10321 tgtataattg tttgagttaa tgacatttga aaatataatt aaaagaatgt tagatagtgt
    10381 accagatact tttgataaaa gggaaggttc tataatatat aatgctcttg ctcctgttgc
    10441 tatagaactt acagaaacat acattgctat ggatgaatta ctagaccaaa cattcgtaga
    10501 tactgctagt tattattatt tagagaagag atgtaaagaa aggggaataa caccacttga
    10561 agccactcat acgattgcta aaggagtttt taacatagat attccacttg attctaggtt
    10621 taatctagga gaatataact atgtagcaat tgagagaata tctgaaaaaa catataaaat
    10681 gaaatgtgaa actactggac ctgtatttga gttaggaaaa ctaataccta ttgaatatat
    10741 agatggtctt gaaactgctg aactaactga aatcttgata aatggagagg atgaagagtc
    10801 agaagatagt ttaagacaaa gatattatga tagcctaaat tcacagagtt ttggcgggaa
    10861 tatacaaaac tatagggatg aagttaacaa aatacaagat gttggaggag ttaaagttta
    10921 tccaacttgg gatggaggtg ggactgttaa gttagtaata attaactcta attttaaagt
    10981 gccaagtgaa gaattagtaa acttagttca agaagaaatt gacccaattg gacaccaagg
    11041 agaaggctta ggattagcac cgattggaca ccgagtcaca gttgaaggag ttacaagtac
    11101 aactataaac atatcagcag aaataacata caaaaatggc tacacttggg agaatataaa
    11161 atcaattgca gaagaagcaa tagacgacta tttaaatgaa cttaacatga gttgggaaga
    11221 tgaagaaaac ttaatagtcc gtatatctca aattgaaact agattactta gtattgatgg
    11281 agtattagac attgcaaata caatgataaa tgatgttaaa tctaatctaa caatagatag
    11341 taacagcata gtagtgagag gtgaggtagt tggataaaga gattaatcta ataaattact
    11401 tgccacaaat tctacaagat aaagaagaat atataaaagt atttaatgta gaaaataaag
    11461 aaataaaaac actacatgat aaattaaagg acctatcaaa tgaccagttt ttagaggacc
    11521 taactataag tggtataaaa agatgggaaa agataatgtc tataactcct aaaagtaatg
    11581 agagtttaga agatagaagg tttaggattt ttagtaaata tataagtaaa ttaccttact
    11641 cagagagatt tttaaggaac tggctagata atgtagttgg agaaggcaat tatgaattaa
    11701 ctattaataa tgctacttat aacatacacc ttgagagtga tgctagaaat caagattggt
    11761 ttgaggaagt tcattctttt gtaagtaata ttaagccatg taatatgact ttagattaca
    11821 ctagagtgct tataagcaaa gacaattata tgaattttgg tataacaacc ctaatgggtc
    11881 aagaaataac tatataccct tggagtccac cagatataga aacttatgga gaaattgatg
    11941 tattaactgg caatggagtt ggataccaag agataacaat attttaggag gtgatatatt
    12001 ggctatagat aaaagttatt acactataat tacagatgta ggaaaagcaa agatagcaaa
    12061 tgcaagtgtc acaggtaata aagtagggtt tgtaaaaatt caacttggtg atggtggagg
    12121 aagtgagtat actcctactg aaagtcagac agaacttaaa aatgtggtat gggaaggcaa
    12181 catcggaaat acaaccacag atgaaactgc gccaaactgc ataatattag agagtttaat
    12241 accatcaagt gtaggtggat ttatgataag agaaatagga tatttagatg atgaaaataa
    12301 tctaattgca atctctaaat ataaagaatg ttataaacct tctatagaac aaggagcagt
    12361 tgttgatatg aaggttaaaa ctgtgcttat tgtatctaat ataaataata tagaacttaa
    12421 aattgaccct acaataattt ttgctacgct caaagatata caggatttag atgctaaaat
    12481 aagcaatgta aacactaaaa ttgatacaac taaaacagag ttaacaagca acatagaaac
    12541 tgctaagaca gagttaaaca ctagaattga cacagaaaat gagaaacaaa atattaaaat
    12601 tgaccagcta atagcaggtg gttcaaatgt tgcatctact caaacaataa caattgatga
    12661 ttggatagat aatcaagaag gtggattcaa agcaactgta acacatggtt tattaacaca
    12721 gagaataact gtaagtatta tagatgctac tacaaaagat aatgtagtac ccgatttcac
    12781 aattatagac gataattcta ttgaggttag aagtggagta aaagttgagt taaatgttta
    12841 tgtgattaat gggaatgcag agactcattt tattaatgcg actgtagatg ataatagagt
    12901 gtctgaaatg actacttatt catcaaaaaa gatacatgaa gaaattggta aggtagcaga
    12961 gcaactgaca ggaattaata gtaatattat atcgactgta aataacgaca tactacctat
    13021 atagaaagga gcgtgaaaaa atggcgttat caatgagtta ttttaattta cctgacaaaa
    13081 gaaaatatac taaaaatctt gcatttaatc catttgcagg tggaaggcaa aattttgagt
    13141 ggactggtgg tgaccatgga ctaaatggtg agtttaaaga aacttgctta agttgcactt
    13201 acaatggttc aacattaaat tggggttcag gaaatgtttg ggtcttgggg gaatatggtc
    13261 aatatacatt tacttataat tgtgaatcaa tgcatgtgga tacacaacaa aaatttccgt
    13321 atacttctaa tagaataata actataaaag gtagacctgt aatatctggt tcagatactt
    13381 cacttgggaa taaaagaaaa ggatttagtg tagattttac tgtaagtgat gatactccaa
    13441 atgttaattt aatagtacgt gcttatcttg atgataaatt aatacaaaac attactcctg
    13501 ttgtacaaaa ttcaacttta acagcaacag taacagatag tcaactaaac tctttatccg
    13561 tagatggaaa tcacaaacta aaaatacaat taaatgatgg atatgataat tttgatagaa
    13621 tttttacttt taaaaaaata gaaaagggaa ttgatatatc aacatcatta gtaactgata
    13681 gccaagctaa gtttactgta acaaaaatat acagtgaatt gacaaaaata gagtgttatt
    13741 tagatgaaac attaaaagag acttttacta cagacttgta ttcagaaaag actataaact
    13801 atgaacttat agacaatgca atacacacat taaaaatagt tgtaactgat gcagaaaatg
    13861 ttgtagaaga aaaagttata agtataagta agaatataat gccattgcaa cctgatgcaa
    13921 cattacaaga tatatcaact aagttaacag agattgggca aggcgttaga aatggtaaaa
    13981 caagcattat aaatacttta gcattaaaga atatagatgc aagtttaaat aatacactcg
    14041 ttgagttatc agagaaaatt aagggaggtt ttgatagtgg agacgctagt ttacaagatt
    14101 tgatgaatca attaacacaa gctaataata ctatatcgca gttaaatact aagtataaag
    14161 ttgctagtgg tacagttact tcttttgcgg atagtactaa aattgcttat ccatatctaa
    14221 ccgacaatgt tactaaacct ggttcttgga ttaaagttag taatttaggc tttaaaccta
    14281 atattttctt tgctgatttt gattactatg atgctgaata taaaaataat tataaacttt
    14341 tccttttcgc ttgtaatggt gttgccacac aaagaggtgt tgatttttca agtgttacat
    14401 cttttatcag aaaaagtggt gatgagtatt ttcatgctaa tggatggcta tatagtaact
    14461 cagaggggga tgtctatttt aataatactg gcgttcagat tccagcttat aactttgatt
    14521 caactcaaaa acatacttat aaatggtatg ctattaaatt tatttaaaag gaggaaaaaa
    14581 catgaatgtt ccaaaccgaa taatatatga ccaaacaggt agaacaatct ttgaaacagg
    14641 cgaatcctgt ggagatgtat tgccacatta tacaataacg gaattgcatt atatagatat
    14701 tgaatatgga agtatagatt atacaaggaa tagagttata ggtataaata tagaaacaaa
    14761 agaaccgatt ttagaagaaa taccagtata tataacagat gaggaaaaga gaatacaaga
    14821 gttagaaaat caattattaa ttgcagaaaa tgagaaagta ggaggattat tataatgaat
    14881 ataaataatg ttgtagtaag aatattagca gaaagaatat taagtaaggg attaaatcct
    14941 ctaaaaaata gagaatttca gttagatgac gtgactaaca cagagtacag aaaagctgta
    15001 gaggattata ttataaaaaa tagtggagta gtagaaggag cagaaccaac tatatagagg
    15061 gttctttttt tattgaaaga aggtgactaa atgactttta aagagttggt taataaagtt
    15121 agaaatcttg tattagaagc aaagaatgta actatagaag atacagaaag taaatttaca
    15181 agtgaaaatg tagaaggagc attgaaagaa tgtatagata gagcagatga ggcttttcaa
    15241 gaagccgata gtggaaaaac acttttatca actgctatcg gctctcctac tacatcagaa
    15301 caaacatttc aagattatgc gaactatatt acaggattta agagcaatat aagcaattta
    15361 gaaactcaat tgaaaagcaa atattctatt agatatggtc ctattgatgg atatgatggt
    15421 aatccttttt ctgctaattt tggcaagagt gcaagttacc ttattgtcta tgtttacttt
    15481 agaagaagtg tatattatta taatcccagt ggtagttctt taggaagtaa tacaggaggt
    15541 tctgaacgtg catggattac tataaacagc aataaaactg gtttttcagt tcattcatat
    15601 gatactagtt atgagtcata tccttttaca ggttattata ttgcttgttt cgcataataa
    15661 attatattat taaaactagg aggatgtatg gaagaaatta gcataaatct actatgtgca
    15721 gtagcaggag ttgtaatatc ctacttagca tttagaaata actcaaacag aaagatacaa
    15781 gatgaaacag aaacaactac aaaattagaa caacaaataa cttttttatg tgagaatgta
    15841 agagatataa agcatgatgt agcaaagttt aatactagtt tcttagatat tagtgaacga
    15901 gttgcaaaaa tagaagcaag tacaaaacaa gcacatctta gaattgatga aattgtaaat
    15961 agaattggag gaaaataaga gatggataat ttaataagtt ttataccaga gcagttgcta
    16021 attttagtag ctgctctctc tattataggt aaaggttgta aaaaatataa acaattagat
    16081 aataaataca ttccagtagt gttactgata cttggaatag gtttctcaat atggatgcta
    16141 ggattaaatc ctgttgcagt cttacaaggt gtaatttgtt ggggagttgc aataggtata
    16201 aatcaaactt acaaacagtt gaaggatggt gaaaagtaat gaaattaaca aaaatattat
    16261 tactcacaaa aatttaaaaa atttcattaa atagagtata cttttatagc taataagtat
    16321 ataataatag tacagaactc gtaaagcatt tatatgctca aatatacggg acattgattt
    16381 ttgaaggaac ccgccaagct tcttttatag ctcaaatagg cgggacagat aaatttaccc
    16441 actaatatta attggtgggt tttattatat aaaagtatat ctatgaaaag gtgaaaacaa
    16501 tggttgaagt aaaagaagaa aaaacatttg atgaacaaat agatatttta aaaagtagag
    16561 gattaataat aaatgataaa gaagatgcta aatttgtatt aagtaatgtc aattattata
    16621 ggtttacagc atatcttcta agttttaaga atgatgatgg ctcatataaa gaaggaacta
    16681 cgtttgaaga agtttatgat atatataggt ttaataagga atttaggata ttattaacag
    16741 atttgttagg gagcatagaa atagcattta ggacatacat tgcatataca ttagcaatta
    16801 aacatggtgc ttgtggatat ctagaaaggg agagtttcaa agatgaaaaa ttctatatta
    16861 attttttgac agcattagag agagaaaaaa gtaataattc agataagctt tttattatac
    16921 accataaaga aaaatatgaa ggaaaacttc ccatttgggt tgcaacggaa ataatgactt
    16981 ttggtatgtt gtcaaaatta tattcaaaca tgttgccaga agatactaga tacataaaaa
    17041 ataatttgtg tagagtgaat actttattag ttaaatcttg gttacaatca ttaacacagg
    17101 tcagaaatca atgcgctcat tatggtagaa tatataataa taatttccgt attataacaa
    17161 taaaaaacga atataaaaag tataacttgg ataataaaaa gatattttct tatatacttg
    17221 ctatgaagca tttgactatg gataaattaa tttggaatag tttttttata aaacttcaaa
    17281 agttaattaa tgattataat aattctatag acttaaagct tattggtttt cctaataatt
    17341 ggatagagat attggctaaa taaaatagtt actttaagaa gtttataaac acttactata
    17401 tgtaagtgtt tttttattga aaagaaggag gaaaataaat aatgaaaata tgtattacag
    17461 taggacacag tattttaaaa agtggtgcat gcacttctgc tgatggagta gttaacgaat
    17521 accaatacaa taaatctctt gcaccagtat tagcagatac atttagaaaa gagggtcata
    17581 aggcagatgt aataatatgt cctgaaaagc agtttaaaac taaagcagaa gaaaagactt
    17641 ataaaatacc tagagttaat agtggaggat atgatttact tatagaacta catctaaatg
    17701 caagtgatgg tcaaggaaaa ggttcagaag ttctatatta tagtaataaa ggtttagagt
    17761 atgcaactag aatatgtaat aagctaggta cagtatttag aaatagaaga gctaaattag
    17821 ataaaggatt atatatctta aatagttcaa atcctacagc agtattaatt gaaagtttct
    17881 tctgtgataa taaagaagat tatgagaaag ctaagaaact aggtcatgaa ggtattgcta
    17941 agttaattgt agaaggtgta ttaaataaaa atataaataa tgagggagtt aaacagatgt
    18001 acaaacatac aattgtttat gatggagaag ttgacaaaat ctctgcaact gtagttggtt
    18061 ggggttataa tgatgggaaa atactgatat gtgatataaa agattacgtg ccaggtcaga
    18121 cgcaaaatct ttatgtcatt ggtggtgcag catgtgagaa gattggttct atgactaaag
    18181 aaaaatttac tatgataaag ggtaatgata gatttgatac actttataag gcattagatt
    18241 ttattaatag atagattata taaaatattt tagtatttct tgaaaatcat aagtatggta
    18301 atatttagat aaatattatt tattattaat aggtatgggg ggagattatg atatttaaga
    18361 agtatataaa atcattactg atagttgtta gtgttacatt gataatgaac aaatcaatat
    18421 tatttgtaaa tgcattagat ttagagagta acaataacat aaaatcttat aaaagaaaga
    18481 atgtaaagga aattgatata agcaataaat ttgttgatga atcctttaga agagatattg
    18541 ttgaaaaggt attagataat agggatgatc cattaacaaa tgaagttgca tcaaaagata
    18601 actataaaat atatgaaagt gatttaaata aaattgagag ttgcttagag ttagatttac
    18661 ataaaaatcc agatgtttct aatccaaaca aaatagataa tttagatggg atagaaaatt
    18721 ttagaaatct agaaaagctt gattgtagtt caaataatct agaaagtgta gatttaagta
    18781 aaaatataaa attaaaatat ctagatatta gtggaaataa aatattaaac attgatttaa
    18841 aaaacaataa tcttattaat gaattacatt gtgatgcaaa taaattatca aatttagacc
    18901 ttaaaaataa taaaaatttg gtaagtttat tttttactag taatgaatta acagagatag
    18961 atttaagtaa taatgtgaac ttagaaaatt tacaatttct taataataat ttaaaaagta
    19021 tagatttaag tcataataaa aagttgaaat ctctaaattg tactaacaat aaacttaaat
    19081 cccttgattt aagtaataat gtttttttga aaaatttaag ttgttcaggt aataacctga
    19141 gtaaattaaa tataaaagga cttacaagtc ttgagtattt gggatgtgct aagaataaat
    19201 tacaagattt agatgtatct acaaatataa aattagattc tttactttgt gggaaaaata
    19261 gtttaacaaa aatagatata agtaaaaata aagaattaaa ttcactggaa tgtagtggtc
    19321 aatatataga aaaaagcatt ataaaaaatg gtgaaaatat agttgtagat ataggaattg
    19381 aaaattcgaa attaaaggaa ttatatccac aaaattataa tatttcaaca ggttttctaa
    19441 catgggaaaa tctttctgac tttccaaata gcataagtta tagtttggaa tatgaatatg
    19501 gtccagtaaa tgatacagag actatggatg taaatatatc aatagataaa ttagtgaaat
    19561 gtagaatcag atatatagat tatgatggaa gtataattaa tgaaaaagaa atatcagata
    19621 attctagtat aaatgaaatt caaattaatc ctcaaagaga aaactatgag tttagtggtt
    19681 ggaggaacaa taaagatgaa aaaatatata catcagagca ggtaagtaaa ttagtttttg
    19741 atgatagtac tgattttata gcaacttata aaagaaaatc aacaggatca agtggttctt
    19801 ctggtggaac ttcaagtaaa gaagatttta atatagcacc atctaaggaa aaaataacag
    19861 gtgcagacag aaatgaaaca tcagtgaaaa taagtcaaaa aggatggaat aaagctgata
    19921 atatagtgtt aataaatgac tctagcatat cggatgcttt atcagcaaca ccatttgcta
    19981 agtctaaaga tgcaccaata ttacttacta aaaataataa tttaaataaa ttaacagaaa
    20041 aagaaataaa tagattagaa gcaaaaaatg tgtacatagt gggtggatta aagtctgttg
    20101 atgaaaaagt agtgtctgat ttaaagaaaa aaggattaaa tgtaattaga atatctggaa
    20161 atgatagata tgaaacatca ataaaacttg caaaagaatt agacaaaaat tctaacctgt
    20221 caaaagtagt agtggtaaat ggagaaaagg gactagctga tgcagtaagt atgggagcta
    20281 tatcagcaaa agaggaaatg ccaattcttc ttactaatca aaatgatgat atgaaagata
    20341 taaaagactt aatagctaat aaaaacatat caaaatcata tgtaataggt ggagaatctt
    20401 tatttaataa taaagaagtg aataatacat taccatctgt aactaaaata gcaggttctg
    20461 atagaacaga aactaactca aaagtaataa gtcatttcta tagcaaagat acacttaatg
    20521 acttatatgt agctaagaat ggaatgaata aacaagacga tttagtagat gccttatcag
    20581 taggagtgct tgcaggtaaa actgaatctc cagtagtatt agttggaaat ggattagatg
    20641 atagccaaaa agagttaatc aagaataaaa agtttaaaaa tataactcaa attggtggaa
    20701 atggaaatga aaaagcattt aatgaagtag aaaacttagt taaatagatt gaaaggtagt
    20761 aactggaaat agttactacc tcctttttta ttcattttca gttttttcaa taattatttt
    20821 tccatcctca aaagtaacaa gaacatccct ttcatcagga gagatattca tctctttaat
    20881 ccatgacatt ggaagtatca atctgggtgt aaaagagcct ttgccacttt tattaaatcc
    20941 aattctaagt tttctttgct ccaataatat cattcctttc tactattaat aaatgccagc
    21001 aataataaag taaataccaa agcaataaga gctttaaaag taaaacttat tattatatta
    21061 ataatattta aaactatagt gataccaaga ataatcatac aaattctata aaatttatta
    21121 ttcatattaa attagaaaat gtgttataat attagaaaga agctacctag aaggaaggta
    21181 ttttcctcct aggattgaaa cttataattc tttaatcaat cttagtattg cgagtactag
    21241 attaattaaa actaatgtga aagagaaagc atttttaatt ttattaaatt tgttttctct
    21301 tttatttttt cttctttcta atctccccac tttcttacct cctttctata tttatattat
    21361 atcatcacgt gacgtgaaag tcaataaata ttacatattt ttcaataaag ttatattgat
    21421 aaactatatt ttttacaaat aaaaataaat ggtgacaaaa ccaaacttgc caccattttg
    21481 ccaccgaaaa taaataatag aataaattta aattaaacat aataaaacat aaacaacaaa
    21541 actgacctta ataaaacatt gaaataccaa cacttgtaaa aaataacaac aataaacaaa
    21601 taacctagaa taatattaca acttaagtaa ataataattt tgaataataa tttacggagg
    21661 tattcatatg tttaaacacg ataaagcatt attaaaagaa gtaaaagtag aaagaccaaa
    21721 tcctcaatat gctgttttaa tgcaagaaca gttgggtggt gcaaatggag agttaaaagc
    21781 agctatgcaa tacttgtccc aaagttttag aataaaagac cctcaaataa aagatttatt
    21841 tcttgatata gcagcagaag agcttagcca tatggaaatg gtagcacaaa ctataaattt
    21901 attaaatgga catgatgttg attataatgc agttaataca ggcgaaatag aaacacatgt
    21961 attaactggt ctatcaccag tcttaataaa ctcatcagga gctccttgga ctgctaatta
    22021 tgttacagtt acaggggatt tagtagctga cttactttca aatatagctt ctgaacaaag
    22081 agctaaggta gtttatgagt acttgtatag acaaatagat gataaatatg taaaagaaac
    22141 aatagatttc ttacttaaca gagaagaagc acataatgct ctatttagag atgctttaaa
    22201 taaagttaaa gatacaggtt caaatagaga ttttggagtt acagaagatt ctagattata
    22261 tttcgatttg tctactccag ggccaaatca caatactaag atagatatta atcctccttc
    22321 ttttgaaaaa cctttaaaaa aataatttga tattttttat gttgttttgt tttagtagtt
    22381 ataaaaatta tttactagaa gaattaatta tatgaaaaat taaatatgta tagttaaaaa
    22441 tgggtataca cttattataa gtgtatatct atttgtttat tgcaaaacat ataataaaat
    22501 aacaaatgag gtggtatttt ggacagtaat agaaaaccta ataatctaat aaatgaaaaa
    22561 tctccatatc ttttacagca tgcttataat cctataaact ggtatagttg gaatgatgag
    22621 gcttttaaaa aagcaaaaga agaggataaa ccaatatttt taagtgtagg atattcaaca
    22681 tgtcattggt gtcatgttat ggaaaaagaa tcttttgaag atgaagaagt tgctgaaata
    22741 atgaatagaa actttgtggc aataaaagta gataaagagg aaagacctga tgtagatagc
    22801 gtgtatatga cagtttgcca agctatgaca ggtagtggtg gatggccaat gaccataata
    22861 atgacaccag ataaaaaacc tttttttgcg ggaacatatt tcccaaaata ttcaaggtat
    22921 aatagacctg gagttataga tttattaaag aatgtatctg aaaaatggaa tacaagtagg
    22981 gatatattaa taaaatctgg agatgaaata atagaagctc ttaaggatga ttttggtgta
    23041 aaaaatactg agggtgattt atcaaaagag atgttgagtt catcagttag agtatttaag
    23101 gccatttatg atgaaaagta tggtggtttt ggtaatgctc ctaaatttcc aagtcctcag
    23161 aatcttatgt ttttaatgaa atattatagc atagaaaaag ataaagatgt gttaaaaatg
    23221 gtggaaaaaa ctttagatgg aatgtataga ggaggcttgt ttgatcatat tggatttggg
    23281 ttttcaagat actctactga taaaaagtgg ttggctcctc attttgaaaa aatgctatat
    23341 gataatgcta tgcttactat agctttttta gatgcatata aaataacaaa gaaagagtta
    23401 tataaagaaa ttgctataaa aactatagat tatgtagtta gagagatgaa ggataaagaa
    23461 ggtgggtttt attcagcaca ggatgctgat agtgaaggtg aagaaggtaa attttataca
    23521 tttaatcctc ttgagataat tgaagttttg ggtgaagaag atggaacttt ctttaataat
    23581 tattttgata taactagtag tggaaatttt gaaggtaaaa gtattcctaa tcttataaaa
    23641 aataaagaat atgaaagaca taatgaaaag atagctgacc ttagtaaaaa ggtatttgaa
    23701 tatagaaaag aaagaacatc tttgcataaa gatgacaaga tacttacatc atggaatgca
    23761 ttgatgatag ttgctttaac aaaggcatat agtactttaa aaaatgacat atatttagag
    23821 tattcaaata agtgtcttaa ctttataaat aataatcttg taaatgagtc aggtagatta
    23881 ttagctagat atagagatgg aagttccgac tatttagcat atttagatga ttatgctttt
    23941 ttaatctggg cgtatataga gctttatgag tcaacattta atatgaaata tttagaaaag
    24001 gctttaaatt tgaatgaaag ctgtatcaac cttttttggg attatgaaaa aagtggattt
    24061 tatatatatg gtaaagatag tgaaaattta atagcaagac caaaagattt gtatgatgga
    24121 gctattccat caggaaattc tgttcaatta tacaatctta taagacttgc gaaaattact
    24181 ggagataata gacttgaaga aatgtcatat aaacaattaa aactgtatgt ggataatgta
    24241 aagagttctc caactggcta cagcttttat atgctttcat tgatgtttga actttattct
    24301 actaaggaaa ttatatgtat ttttaaggaa gattctgatt taattgcatt taaagaattg
    24361 ataagtgaaa attttatacc aaatgcaact tttttagcaa agaaatacaa tgaagaaaac
    24421 actattatag gttttttaaa taactataga ttgaaggatg ataagactag ttattacgtg
    24481 tgtcaaagta atagttgtag tcaaccaatt aatgatttac aaaaattaaa agatatgatt
    24541 ttaggagtag aataatcata atagcgaaaa aggagatgct ttaaaataaa agtctaatca
    24601 tctccttttt ttgtacaaaa aacagaaaaa gagttcctaa gaacacctaa tctatgtaat
    24661 gattgttttg ttaaaaaata aaaatgacat tatagttata taaaatattg tactataaaa
    24721 aagtggtatt atagatgcga tagtattaat cgtttttatt taccctattt cttctttttg
    24781 caaattcttc aagtgcttca gatttaattt tatctatttc ttcatattcc ataaaattag
    24841 catcttcttc aaagccatct tgttcttcac cattaaaacg atacattttt tttagtaaac
    24901 tccataaggt ctctaattca tctttggtaa attcgtgaaa tacatctgcc ataaagttta
    24961 ttccagtcct actacatgtt accataacct tttttcctaa gtctgtaact ttaacattaa
    25021 ttgctctctt atcatgagga cttggaatta catcaacata tccatttttt tcaagattag
    25081 caactagtct atttatattt tgtttacttg tacccatttt tcttgcaata ttatttaatg
    25141 ttgtttcttc ctctggtaaa tgaagaattg ataaaatagt catatactgt cttgaagtaa
    25201 gtataccaaa atatttatct ccttctattt gtattttgtt tgtaagggta aaaagagttg
    25261 catatgtttg attcattaaa aagagctctt ttagttcatt tgaataatcc atgaaatata
    25321 tctcctttat ttttagaagt tgttttgtcg aaaacagaat atcatgaatt aattctaatg
    25381 tcaatatggt gtcaaaatat attaaagtat tactttttta tgtaactatt aattaaatta
    25441 ttgtataatt gtatgtaaaa acttctatgt accaatgata atttctatga ggaattaata
    25501 attcttaaat tttttagaaa gattttgact tgaaaatact tgtaatataa tatataaatt
    25561 aatatactga ataaaatcaa tctggaggga taattatgaa gtgtacaaac tttactttca
    25621 aaggagagga aggtttagat atatatacat ataaatggga agatgaaaat ataaaaaaac
    25681 caaaggcagt tatccaaatt gcacatggaa tggctgaaac tgcacaaagg tatgagactt
    25741 ttgccaaggt acttactaaa aatggttata tagtatatat caatgaccat agaggtcatg
    25801 gaaaaactgc aaagataata gaaaatgttg gacatttagc tgaaaaagaa ggatttagat
    25861 gtcttgtaga ggacatgtat actttaacaa atattataaa gaaagaaaat gaagatttgc
    25921 caatttatct atttggacac agcatgggtt catttgcaag tcaaagatat ataatggatt
    25981 acagtaataa tttatctggt cttatactat gtggttcaaa tggaaagcaa ggaatcattc
    26041 taaatcttgc tcatctgata atcaatcgtg agattaaaaa gtatggaaga cgttctaaaa
    26101 gtaataaaat aaataatttg attaaaaaat agctatgcac acttgtgact ttagtcatga
    26161 gttagtagct aaaatagtca gcatataggg aaacctatat gtagagatag gatagaacct
    26221 atccaatact acttgaattg ctggaaacc
//
LOCUS       DAMKPG010000051        26192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMKPG010000051 DAMKPG010000000
VERSION     DAMKPG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.054"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(187..942)
                     /locus_tag="PC621_002197"
     CDS             complement(187..942)
                     /locus_tag="PC621_002197"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF4109348.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1383..2372
                     /locus_tag="PC621_002198"
     CDS             1383..2372
                     /locus_tag="PC621_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF4109349.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="PC621_002199"
     CDS             complement(2643..3359)
                     /gene="dapD"
                     /locus_tag="PC621_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF4109350.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3583..4332)
                     /locus_tag="PC621_002200"
     CDS             complement(3583..4332)
                     /locus_tag="PC621_002200"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF4109351.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4399..5286)
                     /locus_tag="PC621_002201"
     CDS             complement(4399..5286)
                     /locus_tag="PC621_002201"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF4109352.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5403..6404)
                     /locus_tag="PC621_002202"
     CDS             complement(5403..6404)
                     /locus_tag="PC621_002202"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF4109353.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6829..7710)
                     /locus_tag="PC621_002203"
     CDS             complement(6829..7710)
                     /locus_tag="PC621_002203"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF4109354.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8033..9226
                     /locus_tag="PC621_002204"
     CDS             8033..9226
                     /locus_tag="PC621_002204"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF4109355.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9432..10550
                     /locus_tag="PC621_002205"
     CDS             9432..10550
                     /locus_tag="PC621_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF4109356.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10681..10926
                     /locus_tag="PC621_002206"
     CDS             10681..10926
                     /locus_tag="PC621_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF4109357.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10913..11659
                     /locus_tag="PC621_002207"
     CDS             10913..11659
                     /locus_tag="PC621_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF4109358.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11944..12819
                     /gene="hslO"
                     /locus_tag="PC621_002208"
     CDS             11944..12819
                     /gene="hslO"
                     /locus_tag="PC621_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF4109359.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13210..14010
                     /locus_tag="PC621_002209"
     CDS             13210..14010
                     /locus_tag="PC621_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF4109360.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14537..16777
                     /locus_tag="PC621_002210"
     CDS             14537..16777
                     /locus_tag="PC621_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF4109361.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17191..18726
                     /locus_tag="PC621_002211"
     CDS             17191..18726
                     /locus_tag="PC621_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF4109362.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            18742..19503
                     /locus_tag="PC621_002212"
     CDS             18742..19503
                     /locus_tag="PC621_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109363.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            19841..20709
                     /locus_tag="PC621_002213"
                     /pseudo
     CDS             19841..20709
                     /locus_tag="PC621_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            20903..21154
                     /locus_tag="PC621_002214"
     CDS             20903..21154
                     /locus_tag="PC621_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF4109364.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            21266..22045
                     /locus_tag="PC621_002215"
     CDS             21266..22045
                     /locus_tag="PC621_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4109365.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(22180..22830)
                     /locus_tag="PC621_002216"
     CDS             complement(22180..22830)
                     /locus_tag="PC621_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109366.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(22827..23330)
                     /locus_tag="PC621_002217"
     CDS             complement(22827..23330)
                     /locus_tag="PC621_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4109367.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(23379..23894)
                     /locus_tag="PC621_002218"
     CDS             complement(23379..23894)
                     /locus_tag="PC621_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF4109368.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            24210..24701
                     /locus_tag="PC621_002219"
     CDS             24210..24701
                     /locus_tag="PC621_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4109369.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            24685..26088
                     /locus_tag="PC621_002220"
     CDS             24685..26088
                     /locus_tag="PC621_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4109370.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
BASE COUNT         9704 a         3231 c         4330 g         8927 t
ORIGIN      
        1 gactataaaa taataaacaa tataattcca tataccaata aataagataa tctttaagaa
       61 aaatgattat atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa
      121 tcaattagat attcaaattg atgaatatga atacctaaat agattgagat ttattatgta
      181 caaaacttat cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa
      241 tttagctgca actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa
      301 tgtaagaact tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc
      361 tcttatagag tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc
      421 atgtgtattt tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt
      481 tccactaggg gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc
      541 ttctaacatc tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt
      601 atttgatgat aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt
      661 ttcatcatat ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa
      721 tattggctct aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac
      781 atcagtcatt gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct
      841 agctacacca caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct
      901 tcccattgtt ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat
      961 taataaaaat ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa
     1021 taatattagt gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt
     1081 tcaaaatata gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat
     1141 atcaaaaaga gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca
     1201 tatgatttat ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa
     1261 agatgtataa aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt
     1321 aaaagtgtta ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag
     1381 atatgttaaa agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg
     1441 cagctttgat aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga
     1501 tagtaaaact tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg
     1561 tatttatagt agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag
     1621 ctatagttct agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt
     1681 gggtgaatcc attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat
     1741 cttcatctga tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa
     1801 aaacaatgac tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta
     1861 taataggagt ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg
     1921 ataattataa tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac
     1981 tttatccata tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg
     2041 aaatagcaga tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag
     2101 tgtgtgaagt gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac
     2161 aacgtcaaca agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa
     2221 gtatggtaga gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag
     2281 aacgtaaagc agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac
     2341 aaccagtagt aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca
     2401 ttgatatatt gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata
     2461 taagaatgaa taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat
     2521 tataaaaaag agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat
     2581 tttttaaatt agtatattat tgtttaatta atctttctaa ttaataatat agtactattt
     2641 aattagtcta aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct
     2701 ttcattttta taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta
     2761 gttacaacag caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata
     2821 actgcattag ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc
     2881 tctaaaacac cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct
     2941 ctagctccaa taacagcatt catatctacc atagaacctt caccaataac agctcctata
     3001 tttataacag ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt
     3061 attgcacctg gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa
     3121 tttcttctat catattctac atgagtatct gttatatcat tcccatgttt atctaatact
     3181 tccattatat cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta
     3241 aatttatcag ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt
     3301 ttctttttaa cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt
     3361 tgttaattct ccttgtattt cattatattt cttttattat atacttattt gtcaatttta
     3421 gtatatactt acttcttatt atttatattt tattttagtt agtttttatt gtatagacat
     3481 agtattaaat aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt
     3541 gcatatgttt aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc
     3601 catattataa aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact
     3661 tgcaaagata ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc
     3721 aaatagagca tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc
     3781 atttggttgt ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa
     3841 aacctctttt atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct
     3901 gttgtgatgt ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt
     3961 aaccaatttt actaatacat ttacaccaag agacatatta tatgattgga atacaggaat
     4021 aactttagcc gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat
     4081 tactaaaggt gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg
     4141 agaaaaatct ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt
     4201 aaggtcgcct tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt
     4261 atcttctctt atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat
     4321 aacttttaac ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt
     4381 accatgcatt aatctttact atgcattaac ttttagtcca aagttaacta attctttctt
     4441 taacacttct aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt
     4501 gaatcccaat aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc
     4561 tgcgattaga gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa
     4621 gaatttagct accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac
     4681 tccttgacct cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc
     4741 aaagtcatct ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc
     4801 ttttacagca actatgttat caattttagc aagttcaact ataaggctag gacttatatt
     4861 tacacaagtt cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat
     4921 tgtttcaaaa tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat
     4981 taatagagca tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt
     5041 atcattagaa ccacttccag ctataacagg aactctttta ttaacttttt caacaacata
     5101 cttaatagta gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca
     5161 agatactata gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc
     5221 aaaatcaact ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa
     5281 tatcatataa aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta
     5341 atgcttgttt ttgagttact attaatattt tacaaaatta tatatttata caagtttgta
     5401 tattatacta aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca
     5461 ccttttctta tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct
     5521 cttcttattc ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat
     5581 tcgtttttag caggattatc aactactaca acacctggag cttcttcaag tgtttttact
     5641 aaatcatcta actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt
     5701 tttactggaa ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc
     5761 atagtttcat ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct
     5821 atatgaggaa gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc
     5881 attcctttct ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta
     5941 gagtaaacaa ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct
     6001 tgtatagtag agcagttagg attagctatt atacccttat gattttttat ggcttctgga
     6061 ttaacctcag gaactactaa aggcacatct ggattcattc tccattgact agagttatct
     6121 actactaaga ttccatttga agcagctatt ggagcatact tttcactaat acttccacca
     6181 gcagagaata atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct
     6241 actatatatt ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat
     6301 aaaaatagat tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct
     6361 accattccag tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat
     6421 tttaaattat taaaacatta ttttatttta gttatacaag taagatatgt taatattttt
     6481 atatatccaa aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa
     6541 aggtatactt tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca
     6601 aaaataagtg acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa
     6661 tattttcttc ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat
     6721 actcttagtt acagctacct ctaccctagg ttaataggat attaaaattt gattattcta
     6781 ataataatac atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa
     6841 tccaaaattt gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag
     6901 aggaagtctt aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac
     6961 tggatttact tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt
     7021 tgagccttca atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca
     7081 tatgttagca agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt
     7141 atcatcatta ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac
     7201 ttgagctaag tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc
     7261 ggccacaaca gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat
     7321 tggcaactta acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt
     7381 cttattatag taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg
     7441 actcatatga acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct
     7501 cttattaact ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt
     7561 agtcgcctca cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata
     7621 ttcgattaat tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac
     7681 taaagcaaca gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt
     7741 aattttttaa gtacaataat aattatagta caaattaatt aattttaata ggtatagtta
     7801 atatcttaac tgtttaaata agatatttat attatattaa tattagaaaa attttaaata
     7861 aatgttttat tctcaaaata tgcatattaa tttaataaaa tataccaaaa tggaaataaa
     7921 aaataaattt tatgagaatt taataactaa taattaatat atttaggtta taataaatat
     7981 gtgcgtaaaa tctaacaata agcaataata ccgtaaattt aggaggaata atatggatac
     8041 tttaaaggaa ctttttaaaa taggaagtgg accttcaagt tctcatacta tgggaccaca
     8101 aagagcagca gagagattta agaatgaaaa cccagatgca gaaagtttta gagcaatatt
     8161 atatggtagt ttggcagcta cagggaaagg tcacttaact gactatataa ttgaaaaaac
     8221 tatagcacct aaaaaagtcg aaattgtttg ggaagaagat attataaaag attttcaccc
     8281 aaatgggatg aagtttgaag ctttagataa agataaaaat gtaacagctg aatggactgt
     8341 atattctgta ggtggaggga ctatagcaga agaaggtcaa agaaacagta aaagcaatag
     8401 tatatatcac ttagatacta tggatgagat agtaaaatgg tgcaaagaaa ataataagac
     8461 tttggtagat tttgtgttgg aatgtgaacc aaaagacata aaagattata taaaaactat
     8521 aaaagatgct atgagaaaat ctattgatga tggactaagt acagatgaaa taataccagg
     8581 caagttatta ttaaaaagaa gagcaagtac attttataat gcatataaaa aagataagag
     8641 cttctcaaca cttgtatatg catatgcact agcagcttca gagcaaaacg cttctggtaa
     8701 tataatagtt acagcaccaa cttgtggttc tgctggagta ataccaggaa tattctttgc
     8761 aatgcaggat ttctataatt atgatgacga aaaaataatt gaagcactac ttgtagctgg
     8821 aataataggt aatataataa aaactaatgc ttcaatatct ggagcagagg tagggtgtca
     8881 aggtgaagtt ggagcagctt gttctatggc agcagcagca gtagcatact taaaaggtgg
     8941 aactatagac catattgaat atgcggcaga aatagcactt gaacatcatt taggaatgac
     9001 ttgtgaccct gtatatggat atgttcaaat tccatgtata gaaagaaatg caatggcagc
     9061 tcaaagagct tatgatgcag ctaattatgc attattaact gatggctcac attctgtctc
     9121 cttagaccaa gttgttgaaa caatgaaaga aactggtata gatatgatgg ataagtacaa
     9181 agaaacagct aagggtggac ttgctaaaca tttcttctct tgctaaaaat aaaattttcc
     9241 aataataatt cctctgtttt gatttatgaa acaggggaat tattattaat ggtgaattat
     9301 ttaataatgc aaaatatttt tatattcaat ttagcttttt atttttaatt agtataacat
     9361 gctatataat attttaatta taaagaaata tagaaatatt ttttaataaa ttagaaattg
     9421 gaggagatta catgagagag gcatcatatt tacaagaaca actttataaa cataggaagt
     9481 cattaaatcc tattgcagag attgggagaa aagagtataa gacttcaaaa tatataagag
     9541 attatcttga taaattagga attgagtatg aagtttattt agaaacaggt atagtaggtg
     9601 taatagaagg taaaagacct aaaaaagata ttgcttttag agctgacata gatggtcttc
     9661 atagtgaaga aggggtaaaa catttatgtg gtcatgatgg acatgctact atattacttg
     9721 gattaattga gtatttaaat gataataaag aagacttaaa tgataatata gtatttatat
     9781 tccaaccagc agaagaaggt cctgggggag caaaagcttt aatagatgaa ggagtgctta
     9841 gaaaatacaa tattgatgaa atatatgggc ttcatatata tccagagtta ccagaaggtt
     9901 atgtaggtac aagagaaggc tattttatgg ctcaaatagg agatatcgac atagaaatag
     9961 tgtctaaaag tggacatggg gctatgccac aaaatggtat tgatggtata gtgatagctt
    10021 caaactttgt aagctcactt caaactatag tgtctagaaa tataagccca atagacaatg
    10081 cagtgttgac tattggtaga attgaagggg gagctagaag aaatataata gctgaaaata
    10141 taaagatgga aggtactatg agatgtttca gcccagaagt gtatgagaaa atgaagctta
    10201 gagttagaga acttgctaga gggtttgaat tggcttataa ctgtaaggtt aatataaata
    10261 ttatagatga ttatattgct gttaaaaatg acaaggattt gtatcaagag tttgtagagg
    10321 ctattggaag tgatactgtt gtagagttag agccacttat gatatcagaa gatttttctt
    10381 attatcaaaa agaagttcca ggattatttt ttatgttggg ttcaagaaac gaagaaaaag
    10441 gatttgtaaa tggtcttcat aatataaact ttaattttga tgaaaagata tgtgtaaatg
    10501 cattgaatgt ttattcaaaa ttattaaaat acaaagaagc aatagattag gaagataaat
    10561 tataaagatt aaaaagcata aatacctaaa tttaaaaaaa atgtattaat aaatacccct
    10621 tatatatttg tataaattta ttttaattga caaaaataca aataagaagg ggtgattttt
    10681 ttgaaagaaa aagtacaaag agaaaagaag ataactaatg gcaagaatac taaaatattg
    10741 acaaatgatg atataatgaa atatgagata gcctcagaac taggtcttat ggataaagtt
    10801 ggtgaacgtg gatgggcagg acttacagct aaggaagcag gaagaattgg aggtatgctt
    10861 acatcacgca aaaagaggct taaaaagatt gtggaggagc agaataaaaa ggatgagtca
    10921 atatagtgat tttgcgtttg tatatgatga acttatgaat gaagttgatt ataatggttg
    10981 ggttaaatat atagaagata taatagatag tgaaggtgta aaagtaaaaa atatattgga
    11041 attggcatgt ggcacgggta atcttacaat tccattgact aaaaaaaatt acgatatagc
    11101 tggtatagat atatctgatg agatgcttag tgtagcaaga gaaaaggcag aaaaagaagg
    11161 agtagaatta gttttacttc aacaagatat atcagagctt gattttgaaa tatctgattt
    11221 ggactgtgtg ctttgtgctt gtgatggatt taattatata acttatgatg atgatttgga
    11281 aaatgtattt tcaaagacat atgaattatt aaagaaagat ggaatattca tatttgatat
    11341 aagttctttt tataaattag ccaacatatt gggaaataac atgtatggag aaaatagaga
    11401 agatatagcg tatatgtggc aaaattactt tgatgaagaa gagaatctaa ttgaaatgga
    11461 acttacattc tttattaaag atgaatgtgg aaagtttgaa agatttgaag agatacacca
    11521 acaaagagca tatactgaag atgagatagt tgatatgctt gaaaaatctg gatttaagga
    11581 tataaaatat tatggggatt ttacatttga caaacccagt gaagaaagtc aaagaatctt
    11641 ctttgtatgt aaaaaataag atatttttga ctatataaga taattattaa aaataaccat
    11701 gatataaaaa tatagaaaat gcagttttat agattaaaag taaagtgatg atactttatg
    11761 acttttattt tctaataatt ataaagttct atagataaaa attaaaatat aataactatt
    11821 gcttagtaaa attaaaataa aatgggtttt gcccaaggtt aatatagata agtacttata
    11881 gattaaaatt aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata
    11941 ttaatgagag attatgtact aagagcgaca tcaggaaatg gtcaagttag agcatttgtc
    12001 gcaactacta gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca
    12061 acagcagcac ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat
    12121 gatagtgata aattgactgt aataataaaa ggtggaggtc caattggaac tattatcgcc
    12181 acatcagatt ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa
    12241 gactatccaa atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa
    12301 gttataaaag acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga
    12361 gaaattgcag aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta
    12421 gctttaggtg ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa
    12481 ttaatgccag atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta
    12541 ccttctataa ctaacatgtt aaaagaagga aaatcaccag atgatatatt aaatattgtt
    12601 ttggatggtt tggaacctaa aatacttgat acctgtgaag tagggtttat gtgtgaatgt
    12661 tcaaaagaaa gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcagata
    12721 atagaagaag ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat
    12781 agtgaagaag agttgcttga aattttaaag gaaatgtagg ctataaaaat agttgtattg
    12841 atataagtaa tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagtataat
    12901 ttattaataa tagacatatt tatattaaaa aataaattta ataaattttt aagcttaata
    12961 aattttaaga attaagaagc tttctggctg taatatttgc aatgttgcag ttagaaggct
    13021 tttttatttt attatagttc gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata
    13081 atttgtatat ttagacctga ttgacacaaa ggtttataag atatttgagt tgattatacg
    13141 aaattctatg aagtagtaac cctgtgactt taatcatggg aggttcagaa gaaggagaaa
    13201 attttaaata tggaaagtaa aacgaagtac ataggaataa gtatattaat attattgatg
    13261 atatttttaa ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc tgagagtaat
    13321 agtagaaaat ttaaatcagc agcagtgttt tatcctcaac atcaagatga tgaggtttta
    13381 tggggaggaa gtgccattgt agatgctata aaacagtgtg gtgttgataa tgtatatgtg
    13441 gtattagtat cagatggttc tggagttaac gttttcaaag ctaacacaaa atttagaaac
    13501 ttaactagga agcaaaagga agagcttaga aataatgagt ttaaatcagc tttgagagag
    13561 cttggagtga aacctcaaaa tgtgattata cttgcagata tagataaaaa ggaaggcaca
    13621 cattatgaat taatggaaaa gactatattg gattttgaac ataagtttaa aggcaatgtg
    13681 acccatatag cccatcacta taagtatgat gaccatataa tgcatagaaa gaatggggaa
    13741 gttttaaaga aacttaaacg tgaaggtaag ataaaagatg acttgtattt tgttaagcca
    13801 aaatatgtaa agtatatacc aagaaatgaa agagctatct ataaagcaat ggattttgat
    13861 gactacaata aggttaagag tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa
    13921 atgtatggta taggatatat ttcggcacat agttattttg aacatcttct aaatgaccct
    13981 gaacttacgt ctgtattaag tgaagattaa tttcttataa gtagtaagtt atgtttgata
    14041 agttgattgg aattttctat agttttgaag gtattggaaa tttagtttta tttaaagatt
    14101 taaagataga ggtttttaat gtttaaaatt ttaaagttaa gttgaataat tgtatagaat
    14161 tatttgtgaa agaagaaaca gtgtaaaaca tagtatctta tttatggttt taaaatcata
    14221 ggcaagatac tttttttatg taactaatac cattttgagg ttattaactt aagaatttta
    14281 tataatttaa cactcatata taatgatgat atttaggtag aatagatgca attacgctta
    14341 aataaaataa ctgtattttt tataaaaatc aaaccctaca ctatactacc agataaaatc
    14401 tacatgctat aatttgacct gtgtttataa aaaaaggagt aatctaataa aatagtattt
    14461 cacttatgta taactaacaa ctattattac gattttctct aaataattta aagaaaaata
    14521 atgcaggagg taaaatatga gcaatgaatt tataatttta aaaggttgca aagagaataa
    14581 tttgaacaat atttccttaa aaataccaaa aagaaaaata accatattca caggggtgtc
    14641 gggttctgga aagtcatcta ttgtatttga gacaattgca aaagaatcac aacgccaact
    14701 aaatgaaagg tttagtacat ttgtaagaag ttttttacct aagtatggag aggttaaagc
    14761 tgattgtata gaaaatctgt caacaccaat tatcatagac caaagtagac ttggtggaaa
    14821 ttcaaggtca accctaggta ctattacaga tgtaaattcc tttttaagag ctttatattc
    14881 aaggtttggt agtgagtata taggtaatgc taatatgttt tcttttaatg atattgatgg
    14941 tatgtgccca cactgtcatg ggcttggaaa aaagctagtt cctaatatga accaaattct
    15001 tgatatgaat aaatcattaa atgaaggtgc tatattgctt tcagggtttg gagtgggttc
    15061 ttggcattgg aatatattcg ctgaatcagg attttttgat attaataaga ggatatgtga
    15121 ttattcagaa gtagaattgg aaaagttttt gcatggtaaa gcagaaaaga ttaagataga
    15181 aaatgctggt caaactaaca tgacatatga gggattaatg gttaaattta atcgtctata
    15241 cttaagtaga gatggagaaa tatctgaggc tactaagaaa aaattgagta aacttcttat
    15301 agaagataga tgtcctatat gtgatggaag aagacttaat gaaggagtat ataggtcttt
    15361 aattaatggt tataatatag cagatttaac tagtatgcaa attgatgaat tggctgaagt
    15421 tattaaaagt ataaatgagc cagaagcaca acctttaatt aaaggaataa tagaaaagtt
    15481 aaacagtatc atagatatag gtcttggata tttaacactg gatagagaga catcttctct
    15541 atcaggaggg gaatctcagc gtataaagat ggttaagtat ttaaatagta atcttgttga
    15601 tttaatgtat atttttgatg aaccaagtgt aggacttcat ccaaaagatg tgtataaatt
    15661 aaataatctt ttaaaaaaat tgcgtgataa agggaataca attatagttg ttgaacatga
    15721 cccagatgtt attaagattg cagaccatgt tgtagatgta ggaccaaagg caggtaagta
    15781 tggtggaaaa gttgtgtatg aaggcagtta taaaaatcta cttacaagtg gtactttgac
    15841 agggaatgct ttaagtaaat ttcttcctat taaagaaagt gtaagagaac atagtggata
    15901 tttagaaatt aaaaattgta ataaaaacaa tttaaaaaat gtatctatta agatacctaa
    15961 aggtgtattg actctagtta ctggagtagc aggagcaggt aaaagtacgt tgattaagga
    16021 tgaattttta aagcaaaatc ctagtgctgt acttatagac caaagccctg tttcagcaaa
    16081 ttcaaggtcg agtttggcaa catatagtgg aattatgaac aacataagaa gcatattttc
    16141 aaaaacaaat ggcgtaaatg cttctttatt cagttcaaat tcagaaggag cttgtgataa
    16201 ctgtaagggt tctggaatag tagaaatgaa tcttgctttt atggagagta taaagagtac
    16261 ctgcaatgtt tgtgaaggta aaaaatttaa aaaagaagtt ttagaatata ggtttcaaag
    16321 gaagaatata attgaagttc ttgaaatgtc tgttttagac gcaattgagt tttttaattt
    16381 aaaacagatt aaaacgaaat tacaatgtat tgaaaaaatg ggtattggat accttacttt
    16441 aggacaaact cttgatactt tgtctggagg agagtgtcaa agattaaagc ttgctagtga
    16501 attaaataag gaaagttcag tgtatatatt agatgaacca acaacaggtc ttcatatggc
    16561 tgatattgag aattttataa gtataattga agagattgta gataatggaa atacggtcat
    16621 aataattgaa cacaatatag atgtaattaa aagagctgat tggattattg agctgggacc
    16681 tgaaggaggg acaaaaggcg ggagagtaat atttgaaggt atcccaaaac aattatgtaa
    16741 caataaaata tcattaacag caaaatatat agtataataa ataaaatata ataaaattat
    16801 aatgatattt taataaatat aatgggtata taaaatgata attaaactta acagtaataa
    16861 ataatgtgag aaatattcaa atttaattaa ataaagtgtc agaaatttgt ttaaatttta
    16921 gtattttaac gattctgacc ttttttctta tttttatgga gagtatcgaa gatggagtct
    16981 ctaatagtta cttttttgtg tctacttgca agtttgatta aaaaatggct atacacactt
    17041 gtgacttcag tcatgggagg ttcagattat aatcttaata tataatttgt gaaataattt
    17101 gatgacgatt tgctgatgta taaacaataa agattaacta cattaaaatt taaatcttgt
    17161 taagtaaaac ttataaaagg agagagatgg atgataaatg atattctaac cttattatct
    17221 gaaagaagtg atttttttat aaagctatcc atagaacatt tgatgatttc tattatttct
    17281 ataataattg cgattgtttt gggtggtata attggtatta ttgtaagtga atttcataat
    17341 tcttctaaac ctatattggg aactatcaat tttctataca ccattccatc tatttctatg
    17401 ctaggatttc tgataccatt ttccggaatt ggaaatacta ctgctgttat tgcacttacc
    17461 atttatgcat tattgccaat ggttagaaat acatatactg gaattactaa tgtggataaa
    17521 aatattcttg aagcagctag gggaatgggc agtacagatt ttcagatatt gtataaaata
    17581 aaacttccat tagccctacc tgttattatg tctggtatta gaaatatggt gattatgaca
    17641 attgcattag ctggtatcgc ttcatttata ggtgctggag gcctaggtgt agccatatat
    17701 agaggaatca cgacaaataa cacagcaatg acaattagtg gaagtatact tattgctcta
    17761 ttagctttaa tatttgattt tattttaggg ctggtagaaa aattttttca acgaaaaaga
    17821 aaaaagaaaa ataggaaaaa agtttttgcc attctaggaa gccttattct tatagcaact
    17881 atctttacgt cttatttatt taatccttca aagactcaaa ctattcatat tgctactaaa
    17941 cctatgactg aacaatatat tcttggtgag atgttggata ttttaattga aaaaaacaca
    18001 ggtctaaacg tggaaattac acaaggcata ggagggggaa catctaatat ccagccagct
    18061 atggagaatg gagaatttga catttatcca gagtatacag gtacaggctg gaatatggta
    18121 cttaagaaaa atgaattata tactgaaagt atgtttagta aactacagtc ggaatacaaa
    18181 actaaactta aaatgcaatg gactggtatg tatggttttt ctaatagcta tgcattagca
    18241 gttagaaaag aagttgcaga taaatataat ttaaaaaata tatcagactt aaaaaatgta
    18301 tcttctaaat tgacatttgg agctgaatat gacttttatg agagagagga tggatatact
    18361 ccattgtgta atacatatgg actgaacttt aaagatacag ttgatttaga tattggatta
    18421 aaataccaag ctatcaatga aggtaagatt gacgtaatgc ctatatttac aactgatggt
    18481 cagtatagtg tttcagatgt tatagttcta aaagatgata aaaatttcta cccatcttat
    18541 caatgtggaa atgttgttcg tttggaagta ttagaggaaa atccagaact atcagaggta
    18601 tttaagatgg ttggagatat tttgactgat aaagaaatgg ctaaaatgaa ttatgacgta
    18661 gaaaacaaca atttagaacc tagagaagtt gcttgtgagt ttctgaataa gaaaggtctt
    18721 ttataaattg gaggaaatat aatgacacct attatacaat ttaaaaatat taaaaaacaa
    18781 tataatgata aaactataat agataatctt aatttagata tagaaaaagg agagtttttg
    18841 actgtaattg gttcgtctgg ttctggtaag acaacactct taaaaatgat taatggtctg
    18901 attttgccag atggaggaaa tattttgata aaccaaacag atatcaaaaa cgaggactta
    18961 attaaattaa gaagaagaat aggatattgt gtacaaggaa gtgttctttt tcctcatatg
    19021 actgttgaag aaaatatttc atatgtacca aatctcctaa gtaaaaagaa taaattagaa
    19081 gtaaaaagtg ctgtaaacaa gtggatggaa atagtgggat tgccaaatga tatgaaagtg
    19141 cgttacccat cagaattgtc aggtggtcag caacaacgtg tagggattgc aagagcttta
    19201 gcttcttctc cagaaatttt attaatggat gaaccttttg gtgcagttga tgagattaca
    19261 cgaaagcaat tacaaaaaga gataaaagaa attcataaaa aaacaggagt tacgattatc
    19321 tttattacgc atgatattta tgaagctttg attttgggaa caaagacttt ggtattaaat
    19381 catggagttg ttcaacagta tgatactcct gagaatattt taaatactcc tgctaaccaa
    19441 tttgtagacc agttattgaa tataagaaaa tctataatag atgatgataa tttaaaagaa
    19501 taaatttaaa tttaaaatca aattgttgtg ataaaaacta ttgatttaga gctaacttca
    19561 agttgtatac ttgaagtgga aaagttagat taaggtatag aagagaattt agcagttggc
    19621 tgtagacgag gtaactgctg aaggtgcttt caataaatgg ctagaacctg tatctattga
    19681 agattataat aaattgaaat aagaagctca atatacagag tagataggca cttcaaatag
    19741 aagtgtcttt tgtgtgccat aaaataaatc aaaatattag caaagaatgt gaaaaacaaa
    19801 ataaaataat atgctatctt taaaatagga ggggtgctat atggataaca atttgattac
    19861 cattgaaaaa actttagatt atatagaaaa acatctttta gatgagatgt tagatttaaa
    19921 tgtaatttct aaagagatag gctattcaaa gtaccattta catagaatgt ttacatcagt
    19981 tgtcggattt agtatacata attatataca aagacgtaga ctaactgaag cagcaagaat
    20041 gctcattttt acagaaaaac caattattga aattgcttta atctcggggt atgacacaca
    20101 acgttctttt tcaacagggt ttaaaaaaat gtttaagcat agccctaagt tttttagaaa
    20161 acatcaagat tttttaccac tacaattaaa atttgatatt aataatagaa aaaggttaca
    20221 tggaggtatg gttttagatg ttaaaaaagt taattgtaca gatataaata ttgttggcta
    20281 ttctggtaaa gtaaaaaaag gatttcatgt tattggaaaa tgttggaggg atttgcataa
    20341 aaataaagag ttgatagaaa acaaggtaga cttggatttt ttaattggag ttaatgatta
    20401 tagtaatttt aaagaaattg aaaataatcc tacatttaca tatattgcag gtgttcaagt
    20461 agctactttt ggtaaattcc taatggaatg gaaaaattcg tattaccgaa aagtaattat
    20521 attgttttta cttttattgg gaaaaatgag gatagtatgc aaaatgttgt agaatatatc
    20581 tatcaagaat ggttccttaa ttcaagttgc cagtttaatg aaaataattt atatgatttt
    20641 gtaaaatacg gagaaaaaac tgataagaat ggtttgagtg aaattcagtt ttgggttcca
    20701 atcatttaaa agaagggatt atgtataaag taggtggatt tatataggca aagttttata
    20761 aaagtatgta tttataaata atttatagtc tgtaggaaag aaatagtttt actataagat
    20821 tatgataaaa tacacaatac tatgataaaa tagtactaaa taatagattt aattttgcaa
    20881 aggataaagt gttttaataa aaatgaatgt agaaaacgaa ttacttaaaa acctagatag
    20941 attgcataca acagagttag gcgttgtaag aattaagaaa aatctttctt tggagacaaa
    21001 tgatgtagtg aactggtgta aaactaaaat agaatctcca aatgccatta ttaatagaaa
    21061 gggaaaaaac tggtatataa gtgtatatga ttgtattata acagtaaacg ctcacagtta
    21121 tacaattatt acagctcata aagaaaagaa atgaattgaa aaatacaaag gtcaaaagca
    21181 ttttccactg tttgtagtac ctatgaagaa gtatataaaa taatattact tttgaataat
    21241 aatttaaatt ttggaggttg aaaaaatgaa taatactgta aaagaaaaat tggcagagtc
    21301 actaacttct gatactactg atattttacc ttatttgcct tatttattac aagatttttg
    21361 ggaattagga agttcaccag aacaaatgtt acaattattt agaaagcata taccttttaa
    21421 tgaggataca aaagttttgg atttagcttg tggaaaaggt gctgtatctg tgaagcttgc
    21481 aaaagagttg aatatacata tcaaaggtat tgatttgata cctgaattta ttgattatgc
    21541 gagaagaaag gctattgaat ataaggttga tgctttgtgt gattttgaaa tgaatgatgc
    21601 taatagttct gtaaaaattg agcacgatta tgattgtgtg atttttggag ctgtagggga
    21661 tattttaggt aattataaag aaactataag taagctatta cctacaataa aaagtaaggg
    21721 gttcatttta attgatgatg catatgtagt agataaatct acaaatgaac agcttaattt
    21781 tgagagggtg tatcccactt acaatgagtg gtgtatattg ttcaaagaat taggtctgaa
    21841 actaattgaa tgtataccat gtttagaact tgaaaatgat gttgaagagg aaatgaactg
    21901 gattataact cgtgcaaatg agctaattaa aaaatatcct tctcaaaaag atatgtttga
    21961 acaatatgtt attagccaaa aaaatgaata tactgattta cagcaggacc ttgttggagc
    22021 aacttggatt ttacaaaaag aataagataa atattagact agataatcaa tgttgtagtg
    22081 aattatccca tgtctagtga atataaataa cataaaaaac cgtcaagatg aatagtttat
    22141 tcgtgcttga cggtttttag tatttgttga agtaaaattt tatttcttta gtatatcact
    22201 tttagtcaat ccatttgatt tagcttgagt attccattga tgattcccca taagtttata
    22261 taggaatcta gggaagttag gtgaaataaa aatagtactt ttgttggaat catctatatg
    22321 tatattatca ctaagtatat tttttgaaag ttcttttaat gcaattccta aatctttttt
    22381 aggtccttta cccaaaggta cggattctag tgaaccaaac atctccccag ctccaattcc
    22441 aataccttga acccatgtaa agttgatttt tttgcaccaa tgatctatca tttgaattgc
    22501 caaatgatta tgttcacctt cattgaatcc acagttaact attgcataca ctggaatagg
    22561 ttttttattt tgtgttttta aaaatttttc acatttctct aaaaaatgaa gtaaatgaga
    22621 tggaatacca tctacataaa gaggaaatgc tattattaat ctatcacaat taagtatggc
    22681 attaagagcg tcaggatact tagatgtgct atctacttta aatgtaaaaa ctttattatc
    22741 attttgaata agttgtttta attcagataa aaaatactgt gaattatttt tatttacttt
    22801 tggactacca tttatcatta ctatattcat atggattcac ctcttatatc ctgtatattt
    22861 tcatagaaac aaacagagtg ttcttttata ttgaaattta tagcatttcc atttactaat
    22921 ttcgtagcag tttctttttc agcttctgta atacaatctc catagaatat agcttttaaa
    22981 tttatctgtt tatcatatct gcttttatga cgcatcatat tatcttttat tacaaagtat
    23041 ggaagaatat aattaatgct tttatcaagt acgtttttga caaaagggct aaaccctccg
    23101 taacagcatc tgcttattat aataattcca tcggattcat ttataagttt acccatgttg
    23161 ctgtaataat cttttacaac acattcagaa ggagttttaa tccagcaacc aaaacgacca
    23221 atacatttgt ggatttcttt tgtgtttgga agtattttta tattgtcatc aagcttgggt
    23281 aataactttt ctaattcttt gtcagttaaa tcatgtatta aaagcttcat aaagtccatc
    23341 tccttttatt atgtatgttt atttctaaaa attatatctt atagattttt cagtaaatct
    23401 ttacaccaat taataacaaa attataatag tcaactccat aaatcattgt ggcatacata
    23461 tatgtatatt ggtcaataca atgattttct tctacatcaa ttttttgttt acttaattta
    23521 tttagaagta attctacttc agttattaac atttttaagt ttgtttcaat tatttctttg
    23581 ggtagatact tgtaaaagaa tatattaaca aggtggtctt gctttgtttt agaaaaattt
    23641 ataggtttct ttaaccattc gagaaatact tctttaccag tattagttat ggaatatagt
    23701 ttttttaatt tgctaccatc tataacttcg tgatagtgta tatatccttt tgtttctaat
    23761 cttttaagtg caggatagat acttccaaag ctggcgtcaa aaaagtacga ggtactttct
    23821 gacattattt gttttaagtc gtatccactt aattcttttt ccattaaaaa tcctaaaata
    23881 atgtattcta acatattacc tcctggtatc aatcagatat atatcgattc gatatattta
    23941 ttattacaga taataaatat tacgtcaaga tgctttttaa aataagctaa aaagaattag
    24001 gaattattga taaatatgca aaaaaattaa tataaaaggt aattaaatat caatgaagat
    24061 ttaaaaatag ttaaattaaa gtaagagtct aatatataaa atttatagta actttatggt
    24121 ttaaatataa acttgtgtgc tattgacaaa gtataaaatt agcagtaaaa taattgcatg
    24181 agcaaataga ttattgcgaa cgcaaaaaaa tgaggacaat agatattaaa ggagagaata
    24241 tgagagagct attaaaatgg gtttccatta taaatagata caatacaaat tatattgaca
    24301 ggaaattggc ggatattgga atcaataata gccaatattt ttatgtatta cacatatgta
    24361 ataatccagg tattacacag gacactatat ttgagaaaat tcttgtgaat cctagtaata
    24421 taacgagagc tttggcaaat ttagaaaaag aaggctttat aacaagagaa ccaggtgtaa
    24481 aggataaaag aacatggcat ttatatccta cagaaaaatc tttagaatgt tatgatgaaa
    24541 tactaaaaat aacaaatggg tatgttgagg agttgttgag ttcatttgag aaagaagagc
    24601 aagagttgtt tatgtcaatg ttaaagaaag ttgcattttt agctattgat atgaatgaag
    24661 aagcaaaatt ggaggcgaag gaaaatggaa gaaaaatata aggagccatt ggaatatgaa
    24721 aaggtttcaa agttattaac taagtacgca attcctagta ttatagcaat gttagtaagt
    24781 gctttatata acattgttga ccaaatattt attgggcaag gtgttggtgt attgggtaat
    24841 gctgctacaa atgtcacttt tccactaaca acaatatgta ctgcaattgc attattatta
    24901 ggaattggag gagcgtctaa gtgttcattg gaattaggtg ctaaaaattc agaaaaagct
    24961 acaaaagcag caggaaattc tatatgcttg atggctgttt ttggaataag tttatttata
    25021 gtagtatcaa tatttttaac accactatta aaattctttg gctctacatc agaaatacta
    25081 ccttatgcac acacatatac aaaaattaca tcaattggat taccatttct tataatgtcc
    25141 actggaatga gtaaattgat acttgcagat gggagaccaa aagcttctat gttttgtatg
    25201 ttaataggag cagttattaa tacaattttg aatccattat tcatttttgt ttttaatatg
    25261 ggaattgctg ggtctgcttt agcaactgtt attgggcaac ttatatcttt tggaataagt
    25321 gttcattata taaagaattt tcaacatatt aaattacata agaaaagttt taaattagat
    25381 agtataattt ataaacatat atttgcgcta ggttcaagct catgttttaa tcaattagca
    25441 atgactgtaa cacagatagt tatgaataat acattggcat attatggggc taggtcagtt
    25501 tatggaagtg agattccact tgcttgtgtc ggtatcatta ttaaagtaaa tatgattttt
    25561 atatctataa ttattggtat atcacaaggt atgcaaccaa ttatagggta taattatggg
    25621 gctgaaaagt ataatcgtgt aaaagaagca tataagtttg cagtgggaag tgcaactatt
    25681 atatctgtta ttgcattctt atgtttccaa ttcttcccaa gacaaattac gtctatattt
    25741 ggtaacggaa atgagactta ttttatattt gcagaacatt attttagaac tttcttgttc
    25801 ttaacattta taaatggtgt tcaaatagta tcatcaactt tctttacatc aattgggaaa
    25861 tcagtgttgg gactttttac ttctttgact cgtcaaattt tatttttaat tccattgata
    25921 gtaattttac caatgtttat gggaattgat ggagtaatgt ttgcaggacc aatagcagat
    25981 gcagcagcgg caatggtatg tatatacttt gcagttcatg aaatgaaatc cttgactata
    26041 aagcaaaata atttgatgag taaccataat aagattgcag aggtataaga caatatttaa
    26101 gaagtataga aaattaattt aagaaatata ataaactaat ttaggaagtg tagaaaatta
    26161 atttaaagaa gtataataaa ctaatttagg aa
//
LOCUS       DAMKPG010000052        26053 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMKPG010000052 DAMKPG010000000
VERSION     DAMKPG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.055"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(35..1111)
                     /locus_tag="PC621_002221"
     CDS             complement(35..1111)
                     /locus_tag="PC621_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF4109371.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(1484..1999)
                     /locus_tag="PC621_002222"
     CDS             complement(1484..1999)
                     /locus_tag="PC621_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF4109372.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(2362..3432)
                     /gene="leuB"
                     /locus_tag="PC621_002223"
     CDS             complement(2362..3432)
                     /gene="leuB"
                     /locus_tag="PC621_002223"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF4109373.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(3439..3930)
                     /gene="leuD"
                     /locus_tag="PC621_002224"
     CDS             complement(3439..3930)
                     /gene="leuD"
                     /locus_tag="PC621_002224"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF4109374.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(3945..5222)
                     /gene="leuC"
                     /locus_tag="PC621_002225"
     CDS             complement(3945..5222)
                     /gene="leuC"
                     /locus_tag="PC621_002225"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF4109375.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(5179..6921)
                     /gene="leuA"
                     /locus_tag="PC621_002226"
     CDS             complement(5179..6921)
                     /gene="leuA"
                     /locus_tag="PC621_002226"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF4109376.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(7045..7291)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7439..7885)
                     /locus_tag="PC621_002227"
     CDS             complement(7439..7885)
                     /locus_tag="PC621_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109377.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(7906..8625)
                     /locus_tag="PC621_002228"
     CDS             complement(7906..8625)
                     /locus_tag="PC621_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF4109378.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(8743..10017)
                     /locus_tag="PC621_002229"
     CDS             complement(8743..10017)
                     /locus_tag="PC621_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF4109379.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(10217..11584)
                     /locus_tag="PC621_002230"
     CDS             complement(10217..11584)
                     /locus_tag="PC621_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4109380.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(11834..12298)
                     /locus_tag="PC621_002231"
     CDS             complement(11834..12298)
                     /locus_tag="PC621_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF4109381.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(12303..12932)
                     /locus_tag="PC621_002232"
     CDS             complement(12303..12932)
                     /locus_tag="PC621_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF4109382.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(12966..13853)
                     /locus_tag="PC621_002233"
     CDS             complement(12966..13853)
                     /locus_tag="PC621_002233"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF4109383.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(13882..15120)
                     /locus_tag="PC621_002234"
     CDS             complement(13882..15120)
                     /locus_tag="PC621_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109384.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(15504..16646)
                     /locus_tag="PC621_002235"
     CDS             complement(15504..16646)
                     /locus_tag="PC621_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF4109385.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(16787..17653)
                     /locus_tag="PC621_002236"
     CDS             complement(16787..17653)
                     /locus_tag="PC621_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF4109386.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(17987..19534)
                     /locus_tag="PC621_002237"
     CDS             complement(17987..19534)
                     /locus_tag="PC621_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF4109387.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(19527..20663)
                     /locus_tag="PC621_002238"
     CDS             complement(19527..20663)
                     /locus_tag="PC621_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109388.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(20919..22016)
                     /gene="dinB"
                     /locus_tag="PC621_002239"
     CDS             complement(20919..22016)
                     /gene="dinB"
                     /locus_tag="PC621_002239"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF4109389.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(22406..23911)
                     /locus_tag="PC621_002240"
     CDS             complement(22406..23911)
                     /locus_tag="PC621_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF4109390.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(24173..24523)
                     /locus_tag="PC621_002241"
     CDS             complement(24173..24523)
                     /locus_tag="PC621_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109391.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(24746..24937)
                     /locus_tag="PC621_002242"
     CDS             complement(24746..24937)
                     /locus_tag="PC621_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109392.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(25056..25604)
                     /locus_tag="PC621_002243"
     CDS             complement(25056..25604)
                     /locus_tag="PC621_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4109393.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
     gene            complement(25747..>26053)
                     /locus_tag="PC621_002244"
     CDS             complement(25747..>26053)
                     /locus_tag="PC621_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4109394.1"
                     /translation="QVKNMIKNRKLSRLISDVSWSEFVRQLEYKANWHGRQIVKVGKF
                     FASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         8506 a         4698 c         2737 g        10112 t
ORIGIN      
        1 aaatttatct taaaacatac atatttttac aatattattt attaaaacct aatatatcat
       61 caatattatc taataattct tgtacatctt cgacagtata gtcacccata tgtgaaattc
      121 taaaagtttt ttcttttaaa tctccatagc catttgctat ttggaatcct cgttcttgta
      181 attttgaatt taaatttgat acactaatac cttgagtatt ttctataaca gtaagtgtat
      241 tggataagtg attttcattg gtaaaaattt ggaagtgttt tttagcccag tttctaacta
      301 agtttgccat gtcttcatgt ctgttgaatc tgttatctag gccttcatct aatatattat
      361 ctaattggaa atctaaagcg aacatgtgag aaagagatgg tgttgaaggg tattggtagt
      421 ttttcttgat taagtattca tacattgcta acaaatcaag atatactcct ctaaaaggta
      481 cttgcttagc tctatcgatt gctttttgag aaaatgtgca aatagacatt cctggtggta
      541 atcccagtgc tttttgtact gatgttatac aaatatctat tccaatctta tcaacctcga
      601 cttttattcc accggctgaa cttacagtat ctacacagta gattacatct tcatactttt
      661 taacaacttc tccaatttct tctattggat ttcttattcc agttgaagtt tcattgtgag
      721 ttacagttat taaatcgtat tctccggtag aaagcacctt atcaaccatt tctggcgttg
      781 ttgcctctcc taattcaact ttaaatatat ccgctgggac attattagcc actgccattt
      841 tgtaccatct atcaccaaag gatccacagg aaaatactgc tgcctttttg gatgtacagg
      901 aacgaatgga gccttccatt aaaccagttc cggatgacgt ggataaaagt atcgtgttgt
      961 ttgtatagaa tagtttttgc atcttttcac ttatactctt ttggagcata gatgcatctt
     1021 ttcctctatg tcctatcatt ggtgtagcca tcttttgcaa gacttcttct ttaacatcaa
     1081 taggaccagg aataaataat ttcttactca ttataattcc ccccttttta gtttgtaata
     1141 tggatagcgt ttttactgtc cttattttta ataactataa tatcaagctg aattaactat
     1201 gtcaacataa aatttatttt ttatatataa tttattttat atattttatg aaaacattca
     1261 ttttctgcct tttcataagt ctgaattttc tgaattattc tctcaaaaaa aatatacttt
     1321 ttatctatat aaattaattt ttttagtatg taaatttgac gctattacga taaatctttt
     1381 tagtataact aatttatttt tatcttgtat ttttattgta tttttgaatt ttattttttc
     1441 taaatataaa atttatgttt taaaatataa aatttttatt taatcaaagt tcaatatatt
     1501 ccactttttc tttcgattct tcaaaaaacc attcttcatt agaaaaatca ccttctgcaa
     1561 tttgtccaat aggcattttc atatactttc cagttgctgt cactgctata tctccatttg
     1621 gtagaattat ttctcctgta ccttcaaata attttctgtt ttctttagtt attcttccaa
     1681 caacttttat agtagcatct gttggtactg gtttcttata tttcaactct aaagatactg
     1741 ttactcccca tatttggtca tcatttatac ttacagctct tccaatagtc tcatctaata
     1801 ttgcagcaga catacctcca tgaactctcc caggataact ttgatgccaa tcctttgtat
     1861 tacaaattga tacaagctct ccattttcaa gttcataaaa atctgcttta agtcctagct
     1921 cattttgtgt tccacaaact aaacaagatt tactactatt ttgcttcttc aaaactttat
     1981 actttgccaa aaaatccatc tcctaaccga cattagtagt cgcttaatat taaataattc
     2041 taaaatatat tatataatat taataaaatc cctgcaaact tctaataata aaaataggct
     2101 acttcaatat gataagttta atttaaagta ttattttcca aaaaataact ttatcaaaat
     2161 agaaactcaa agtagatata agcctctttt tcgctattaa aatagtaaaa atatatttga
     2221 cttaatataa aaaagagtgt ctcattaact aataaagttt attttgagac acccctcata
     2281 aaacaaatta tactacaata gatttttact aattctacaa gttatatatt taaattatat
     2341 tattcaaatt aaactataat tctaaatttc atttactatt aattcgccca ttgctcttgt
     2401 tcctacaaca tttcccacac cattatagat atcaatagtt ctataacctt tattgagtac
     2461 actttgtaca gcatcttcaa tacacttagc ttcttcttct aagttaaaac tatgtcttaa
     2521 catcatgctt acagatagaa tagttgcaat tggatttaca atatcttttc cagctatatc
     2581 aggcgcactt ccatgtattg gttcatacat accaaaacta tcttctctaa gtgatgctga
     2641 tggtaacata cctatagaac ccgtaatcat agatgcttca tcagatatta tatctccaaa
     2701 tatattatta gtcactatta catcaaattg gctagggtct tttacaactt gcatagcagc
     2761 attatcaaca tacatatgtg acagttctac atcctcaaac tctttagcta agtcttccat
     2821 tatacttctc caaagtcttg aactctcaag cacatttgcc ttatctacac ttataagttt
     2881 tttatttcta tttctggcta tttcaaaagc tctcttacca attcttctta tttcattttc
     2941 gttatatatt tctacatcat aagcattttc gataccatca atgatttttc ttccacgttc
     3001 tccaaaataa atgccccctg ttaattctct tacaacaaca aaatcaatgc ctttctcaac
     3061 tatatccgtt ctaagtggtg aagcttcttt tatactttca tacattgtag ctggtcttaa
     3121 atttgcaaat aaacccaatg actctcttaa ttttaaaaga ccagactctg gtcttttatc
     3181 tcccttacat ttatcccact ttggtcctcc aacagcacca agcaatacag cttcgttttt
     3241 tcgacaagtt tcaacagttt tattaggtag aggttctcca atttcatcaa tcgcgcatcc
     3301 tccagctaat acatattcac aatcgaatct atgattaaat ttacaacaaa ttttatttag
     3361 aacctttata ccttcatcta taatttccgg tccaacacca tcacctttta tgactgctat
     3421 attacaattc atactccact accccctatt tgcttttatt gagttaatta agccttcatt
     3481 taatataata ttttgcataa actcaggaaa tggttctgct ttatattctt tttcttttgt
     3541 tatatttttt atcacacctg tagaaaaatc tacttctata ttatcccctt catctatgtt
     3601 attggcagct tcttcacatt caagtatagg aagccctata tttatagagt ttctaaaaaa
     3661 tattctagca aaagtagaag caatcacaca ggatactcca ctttctttta ttacaattgg
     3721 tgcatgttct cttgatgaac cacaaccaaa gtttttagtt gcaactataa tatctccttt
     3781 ttttacctta gaaataaatt tatcatcaat atcctccata caatgcgttg ctagttcttt
     3841 ataatcagct atatttaaat atctagctgg tattattaca tctgtatcta tattgtcacc
     3901 gaatttaaaa acacttccat tggcaatcat aaattttacc tcccttaaat tatttctgat
     3961 ggttttgata tttttccagt tatagctgat gctgctgcta cagctgggct tgcaagatac
     4021 acctctgaat caacatgccc cattcttcct acgaaattcc tatttgttgt tgaaatggat
     4081 ttttctcctg ctgcaagtat acccatatgt ccccctagac atggaccaca agttggtgta
     4141 gaaactgctg ctcctgcctc tataaaagtg gtaatatacc ctttttctat agcttcaaga
     4201 tatacttttt gagttccagg aaatatcata actctaactc catctgctac ttttttgcct
     4261 ttcattatct cagctactac ttcaaggtct gatattcttc catttgtaca agaacctatt
     4321 acaacttggt ctatctttat atccttatct atctcatcta ctgttctagt attttcagga
     4381 agatgaggaa aggctactgt ttcttttagt tcggctaaat ctatttcgta tacttcatca
     4441 tagactgcat cttcatctgc ttcatatata tttacatctt ttgttatagt tgaacactta
     4501 tgtgaattta tatattccat agtcttatcg tctactggaa agattccatt ttttgctcct
     4561 gcttctattg ccatattaca aatagtaaat ctatcatcca tagataagta ttctactcca
     4621 tctccacaaa attccatcga cttgtaaagt gctccatcaa cacctatctc ccctattata
     4681 tgaagtatta tatctttacc acttatccat ttatttggtt tattcttcaa aacaaatttt
     4741 attgctccag gaaccttaaa ccaacatttt cctgtagcca tacctgctcc catatcagtt
     4801 gaacctacac cagtagaaaa tgctcctaat gctccatatg tgcaagtatg agagtcagca
     4861 cctataatta catctccagc agttactata cctttttcag gaagaagaac atgctctatt
     4921 cccatatctc ctacatcaaa aaaatttaca actccttttg attttgcgaa acttcttgta
     4981 aatttacatt gttctgcact ctttatatct ttattaggtg taaagtggtc taaaaccatc
     5041 actactttag tcttatcata aacatcttca attcctaatt tttcaaattc tctaatggct
     5101 actggtgatg ttatatcatt acctaaaact acatctaaat ctgcttcgat taattgccct
     5161 ttcttaactt catttaattt agcatgtttg gctaatattt tctgcgtcat agtcatcccc
     5221 attttttatt tcctcctcaa tataactttt atttattgca ttcataagtg cattaatagc
     5281 tgctgtattt atgttttccg atatacttac accaaaatag acttcattat tgtgctctat
     5341 tcctacataa gctacccctc ttgaatgtga cccttcctct aatgcatgtt cccagtaata
     5401 tctaaattta taatctttca tgccacattg ctttagtgca ttattaaatg aatctacagg
     5461 tccatttcct ataccaacaa tcctttgttc atttcccata taacttattc ttgcagtcat
     5521 ctcaatcatg ttagtatcat tagaatcatc attttcatag tttaattcat ccattgattt
     5581 tattttatac ttcttaactt tataagggct ctcaacttca atatattctt tcttaaataa
     5641 attaaatatt gcttcactac taagttcttc cccaattcta tcagattcca ttttgactac
     5701 atttccaaaa tcagaatgca tattcttagg tatcataaat ccataatctg tttccattat
     5761 ataaacagct cctccttttc cagattggct atttatcctt ataatttctt tgtattctct
     5821 tccaatatca tgagggtcta ttggtaaata tggaacttcc cagtaatcat tatctctgtt
     5881 tttcattgct atcatgcctt ttctaatagc atcttgatgt gaacctgaaa aagcacaatg
     5941 tacaagatta cctgcatatg gatgtctatc atgtaccata agttttgtac agtcttcata
     6001 tataccaatt atcttatcta tattgctaaa atctagctct gggtctattc cttgtgtata
     6061 aagattaagt cccacattta ctatatccat atttccagtt ctttcaccat ttccaaatag
     6121 tgttccttca agtctatcgg caccagccaa taatccaagt tcacttgcag ccgtacaagt
     6181 tcctctatca ttgtgagtat gcaaacttag tataatagaa tccctacaac ttatattttt
     6241 acaaaaccat tctatttggt ctgcatatac gtttggtgtt gccatttcca ctgttgatgg
     6301 aagatttatt attacttttt tttgaggagt tggtttccaa acatctatca ctgcttcaca
     6361 tatttcaagt gcataatcca tctctgttcc tgtaaaactc tctggtgaat attcaaaagt
     6421 aaattctgtt tctggatatt tttttacttc ttcattaaat aattttgctc cttcaactgc
     6481 tatatctatt atttcttgtt tactcatatt aaaaaccaca tctctttgaa gaacagatgt
     6541 agagttatat aaatgtataa ttgctttttt acatcccttt attgattcaa atgttttttc
     6601 tataagatga gccctagatt gtgtaagtac ttgtatagtt acatcatctg gaatcatatt
     6661 ctcatctatt aattttctta aaaatgtata ctctgtattt gatgctgatg gaaatcctac
     6721 ctctatttct ttaaatcctg tatcaataag cattttgaac attctgactt tttcatttac
     6781 actcataggc gttggcagtg actgatttcc gtctcttaaa tcgacactac accaaattgg
     6841 agccttaaat atttcattat ctggccattt acgatttact atttgcattt tatcatattt
     6901 tttatacttt ccacacttca tgttatttcc ccctttttct gttttttgta ttcagtatac
     6961 cttatatcga cgcaatcctg cacacttcat aaggataaat gtcaatttta tcattagaat
     7021 aaaaaaaacc tcatctctaa taaaattaga gacgaggttt tgctcgcggt accactctat
     7081 ttagctttct aaaaagctcc cttagtaata gccaatacaa taattataat gactatattt
     7141 cctataacgt ggaattactc cgattattct aagatttaaa tcttagaaaa cagctcctag
     7201 acaagttcag aactatcaac tactaaatca caccatcctt tagctctctg aaagtttcat
     7261 tgttcttact acttctattc atcacttttt taatcattta aattttctaa atattttgat
     7321 ttaatagtta ttatatacac attaaaatgt attgtcaaca aaaaaaataa ttatctcaat
     7381 tttttatttt tgataagcaa ttctataaaa catgctaact attgaaaaca gtatgatatt
     7441 atgccttaaa ctttttcatc ttttcatcta tgatattcaa taattttttt tcatcttcca
     7501 aactcaatgc ccttaatcta attttataat tagattcgtc agtgtatata tttaatatat
     7561 tttttactac ttctattttt tctattatct tataattaaa cttttgactt tgtttagatt
     7621 ttatactgaa tatacattga ttatttatta atattgaatg attatcgaga gtaatatata
     7681 cttttccctc atatttattt gaaaaatcac gaccttgttt aaatagaaaa actgctgtta
     7741 ttatacataa aatatataat attgtcgttg aatatataaa aattgtagct aaaattataa
     7801 gtgctaaaac catatatata gtcattcctt ttttaaaata accattgtca taataattca
     7861 tatagataaa cttattttta ctcaaatttt ttcactccca agattctaaa aatttatatc
     7921 aaaaaattct ataatttcat cttcatgata atttatgact aattcatctg gaaaatctac
     7981 tgcttctaaa actttttctg cattttcaaa ttctcctata gaataacata tatgagagtc
     8041 agtaccaaga attactcttg taccatatgt tttacacaat gtcaacatat gacttacatt
     8101 ttcccaagta cccgttctat gagagtttga acttaatgat gaattattta tttctaaaag
     8161 gatattttta tctttagctt tttttactat aggttcataa tctaaaggat acctactgtc
     8221 atctggatgt ccaattattt ttaccttagg tttatccata acttttaaaa tagcatttgt
     8281 attttcttct tttgtaccag aagctataca aggtctatgc aaacttgcga ttatataatc
     8341 caaatgttgc aacatatctt cttgaacatc taaattacca ctataatcca ttatatttcc
     8401 ttctattccc ctaagaatct ttacaccttg cacttcacgt ggaagcaaat gcaaattata
     8461 aaaatagaat ggatgtggtc ctcctggcat attaggtccg tggtctgata ttcctaagta
     8521 ttttatattc ttcttactcg cctcttcgac attttccttg aaagtactat gtgcatgtcc
     8581 acttattaga gtgtgacaat gtacatccat aatagacttt ttcattaaat ttcctcctaa
     8641 tattttacat aaattcgtga aaatagctaa agggctattt caaaactaaa gctgtcataa
     8701 tagacagtgt aacgttttaa aacagcccca ttttcaatta ttttacactt ctaaatcttt
     8761 aaatttatct ttaaataatt ctccaatact tcctaaagat tcctctactt ctaggtattc
     8821 tgttatatcc tcttctggct ctctagttgc ttcttttatt gaaaccaaca ttcttttagt
     8881 ctttggttta aaatcaagaa tcttaacttt aacctcatct ccaatattaa caacattagt
     8941 aactttagcc actctagtat cactcaattc agatattggt aaatacgcat ctacaccttc
     9001 tttaatttga acaaaagccc ctttttctat tattctaaga actttagcat tgacaacatc
     9061 atcaatttgt acttcttttg ctattaaatc ccaagggtca ttatttatgt cttttaatgc
     9121 caaggcaatt tttttagtct ctttatttac atccaacaca taaacttcta cttcttgtcc
     9181 ctctgaaaca acatcttcaa cactttcaac tcttgtccaa gctaaattat ttatatgtgc
     9241 taatccttca atcccaccaa tatccacaaa tgcaccataa ggcattatct ttgtcacctt
     9301 accatctctt ttttgtccaa cttcaagaga attaaataag tcttctttag ctgctttgat
     9361 acgagctttt tcttcttctc tttcttgctt aattcttgct ctttcagctt ctttttctgc
     9421 cttaatttga gctcttcttt cttctctctc tttatttata cgctcttgta atacatctct
     9481 atgagatgct acaagtctat tttttctaac atccaattct tttatataaa cttctaagtt
     9541 aagaccaaca aatttagatg tatctgtaat aaattttgta tcaagttgag aaattggtat
     9601 aaaaaatgta tatgtattgt aattagcaaa tacaccttta tctatattct tttcaacatt
     9661 tacagttatt attctatgtt catcataagc cttttgtaac tcggcaaaat catttctttt
     9721 gtcaagttgt aatttagata aagttattgt accatctttt tgagatactt ttgttattac
     9781 agcagttatt tcatcaccta tgtggtatat atcttctata tattgatttt tttctatgtt
     9841 caattcactt ataggtatta ttccatcaaa cccatagtct aatcctaatt gtacttcatc
     9901 atttctcact gcagttattt ttccagttgt caattctcca actttcactt tgctgaatac
     9961 ttgttcttgc tggtcaagta attcttgcat tgttaaatca ttttccatta taaacatcct
    10021 ttcctcttat tgattatcat atctgtaaat attataccat taatattgca aatacttaca
    10081 tttttcttac tataattttt aaaataaatt caacttcata tatttataca ttatagaaaa
    10141 tatcttatta aattaaaata caaattctaa tttttatcta ataaaatatt ttcataaaaa
    10201 atttatattg tatatcttat tcttttattg ctttaggttt tgatagaaaa catttataaa
    10261 atataaagaa agaaattgct gttatagtag cagctgttat atctgcaact ggctctgcta
    10321 aaattactgc aaaaagtttt tcattaaaga atgttgggag tataaatatt aaaggtatta
    10381 aaagtacaat ctttctaagt agagctaaaa ccaaagatat tgttgctttt ccaagtgcta
    10441 aaaatgtctg ttgacaagct atttgaatcc caaacataaa agcaccagca aaatatattt
    10501 ttatactcca actagtcata ctaagtaact gcggattctt attaaatata ctaacaaaaa
    10561 atgcaggaaa tatcattaat aaaagccata aaatagtaga atatgtaaaa catgacaata
    10621 aacacaactt aaatgtcttt tttactctat caagttgacc tgaaccataa ttataactta
    10681 ttattggttg tgctccttgt gtaaccccca tattaggaag cataagaatt tgcattatac
    10741 tgctcattat ggccattgaa cctactgcta aatctcctcc atacatttgt aatttactat
    10801 ttaaggctat aagtactaaa ctttctgtag attgcattat aaatggagag attcccaagc
    10861 caagcactgg taagatgata cttgcttttg gtatcatata cttctttttt attttaagta
    10921 tacttttctt tccaaagaga aacataagta cccaaatagc agaaacgcct tgtgcaatta
    10981 tagtagctaa tgcagctcct tttacaccca tattaaagcc aaatataaaa atcgggtcaa
    11041 gtattatgtt tattaaagca cctatagcaa ctgtcatcat tccagttgta gcaaaccctt
    11101 gggtatttat aaaagtattc atacccattg aaatctgaac aaacactgta cctattaaat
    11161 aaatatttaa atagtccatt gaatatctaa ttgtagattc acttgctcca aacatccaaa
    11221 gtaaaggctc ttttccaatc ttaaaaccta tagtcaaaag tattgctaaa atcaccagta
    11281 cagaaaaact gttactcatt attttctctg cctcatcatt gttttgctca cccattttta
    11341 tagcagctaa tggacctcct cccataccga ttaaataact acatgcagtt attataatta
    11401 ttattggaaa ggccactcca acaccagcca tagcaacttc tccattttcc attctaccaa
    11461 taaaaatcct atcgactatg ttgtataaaa catttacaat ttgtgcaata atagcaggtg
    11521 tagcaagttt aaataataat ttacccacac tttcactgcc taagttagtg tttgaatttt
    11581 ccattttaaa acctcctcaa tccaataatt aaaatgtatc actaaaattt tattctatat
    11641 aaaaatttca ttttatcagc agacaaattt agagtacttt ccctacaagt aatactatac
    11701 tatatttctt ttgccatagt aaacatttta gaactaactg aatttaaata ctttatctca
    11761 ttttttattt attattaaaa atttttcaca tgtactcata aaaaaactat ccaagtatat
    11821 tacttagata gttttatatt atatgtaaag aaagagctga aactataacc cctgataaca
    11881 aattgccaag aaatactccc ataaaagcat cttttattct catttttaat atggaagcta
    11941 tagcagatgc agtccatgtt cctgtagttg gcagtggtat acctacaaat agtattatac
    12001 caattatcga aacacttttt aatttcttca ttcttgagtt tatttttcta tcaattacat
    12061 cagcaatccc tttaaatagt ttcttccctc ttaaaaattg taatacatgt ctgaatgtca
    12121 atatcaaggg aatcgatacc aatgtagaac ctattacact aaatacataa acccatatag
    12181 gatttaagcc cattgcaata cctataggaa tggctcccct caattctgtt acaggaacca
    12241 tagataaaat taatatgtgt aaatatccca aactattccc tcctattcta agtgacatta
    12301 agttatgaat ttatttgctc tataacctca ccatcctttt tactaactaa aaatgcattt
    12361 ttagtcaatg aaagcattgg taaatcactc ttagagtctg aatatgcata tgaattttca
    12421 taatcaatct ctatcccaag tttacctaat acagccttta ttctttttac tttttcaatt
    12481 cccttacagt tttctccaat gaattgattt ttatatcttg aattttcata aaaaaactcc
    12541 gtgcctataa ccttgtctgc aagaccataa tacttaaaat atttcatata tgcatatgga
    12601 gatgcagtta tcataattat aacattatct tcttctttat ttttgtaaat gacctttttc
    12661 atagattcgt taattttttt cattatgcaa tcatcaaaaa acttttttaa atcttcttca
    12721 ctaaaatcta ctattggata aaagattgcc tcttttgcct tcttcaaggg tatcattcta
    12781 attaaataca taaatagaat aaaaataatg tatggcaact ttataatagt tttaaaggtt
    12841 ttatttttta aagagtaaat aaaaaaatct atcatgctat cagtatttat aacagtataa
    12901 tctacatcaa gtaataatac actttttttc ataagtattt ccccatccta tcactaaaaa
    12961 tatcactaaa aaatcaatat tattaaacat gtaattatcc ataagattac gtttattttt
    13021 aaagcattat cttttaggac aatatcagta ggattatttt catttgtagt gttaattata
    13081 taattatatc taagtacacc ataaactaca aatattgttg taaatatcat atgtggcttt
    13141 tctggattac ttgtagaata caatgaataa catacaattg tagaacttaa tataactatc
    13201 atcatttgat ttagattttc tatgtcataa tcttccaata tttttctatg ttcaactgca
    13261 tcatctctta gagtttctat ttctttttta cgttttccaa gagctaagta cagagataag
    13321 aaaaatgtac ataaaattat ccaatttgat aaactaacac ttatggctac acatcctgca
    13381 taaaccctaa gtataaaacc aagagatatt gaaaacacat ctaatagtat tatattcttt
    13441 agtttaagag aatataatat attgtttatc aaatagagac taagaattat agaaagatta
    13501 aaactaacaa ctgttgataa acagactgaa attaaagcta atactacaaa tagtatatta
    13561 gcactttttt tgcttattct tccacttgcc actggtctat tacatttttt aggatgtttt
    13621 ttatctttat ccatgtctat agcatcattc agtgcatata ctgatgatga cattaggcaa
    13681 aaacacacaa acgcaataaa cgtatttaaa aataaattta tatttagtat gttgtttgaa
    13741 aaaattatag ctgaaaatac aaagaaattc tttatatatt gctttattct cattaacttt
    13801 atataatctg aaattttagt aagaagcctt atacttgtat tggtctgttt cataaactta
    13861 ctaccctttc aatattattt tttaaacagg atatgatttt atccctatgt ttaaaatttc
    13921 tataaatcca ttgacattac tgattactat agtaaccaat aacagcgata gaaatatatt
    13981 atacatattc ttttcatttt ttctaagcgc tttcgtaaag taagccaata aaattataac
    14041 tgcaaaacta aaatttatag ccataatact tgaattgact agattatatc ctactatacc
    14101 atgtaaagtt agattgaata taattattcc catacatagc ttaggaactc ttttttctga
    14161 attttcaata actgataaaa cacaaaaaca taaagctaat aatgtaagaa ttactggtat
    14221 tgtagcaact tctccatttt gcaaaaataa ccctttttta atacttgctc caggaaaata
    14281 aaaaaccgaa tacaataatc tctctataat cattgcaatc ttttccaaaa ttggttctcc
    14341 acttataaat ttcgtatcat actgtattac atttccaagg ttcataaaac tctcataaga
    14401 cttcgttgaa actacacaaa tatatgccat taggataaat gtaattaaat gttttaacat
    14461 ttttcctatt tttaatttaa actcaaatat catattaaaa aaatacaaat atatattact
    14521 aatagttatt ccaaatagag gaatagctgc taattctact aaataagaat tttttccttt
    14581 catcttattt gcaataacta tgaaaaatat taatgaaaat tggctatata taaatctctc
    14641 tacatttaaa gtcataaata actgtggaaa agaaaatatc attatcatag taagtaaaat
    14701 taaagttaat ctctgcattt ttatcttttt aatatgatta aagactaaag ttattgacat
    14761 agcactagtt attatttgta gacaaattag tccaaaccca taggcactat taaaatctgt
    14821 tgtcccagga tttgctattg aagtacaaaa taggtatatt ggatatgcaa aaattgatat
    14881 aattgctgaa aacaatatgt gcttactatt gtcttgactt atagcaaata catttaaatt
    14941 gaataaaacc cctgtatctg tatctaacaa tacatcataa gtattgttta aattataaaa
    15001 tatagtagtc ttagaaaaaa atatcttagt tattattaag taagttatta caactaatat
    15061 aaacaaaaac atctcgtttt tatattttaa tattttactc atcaatatat caccatccat
    15121 tcattatata ggatttgctg acacctacaa tttattcctt atatttaata gccatcttca
    15181 taatgttctt acttgtttta ttgtaattga ataactcttt atttacaacc acaatattta
    15241 ctttattgat gttataatca gtcaagtatt gtttatataa catagattag tgttgtataa
    15301 aatgagaatt tgtaagcaat attaatgtaa aatatttgaa aaactttata taaaaatata
    15361 caatgttgct tatggaatta aactataaca aattaaagca taatttaagg agacaaaatt
    15421 ataatctgtc tccttaaata ttaaaactat attttaaaac attactaaat actatacaaa
    15481 tttcaaatat agtataaaat taattagact ttaacactat tagctccatc ttcttcccat
    15541 tttttaattt cttttaatct atctattata ggtaaatgct gagtacattc aacttcacat
    15601 tttccacaag atatacattc tttagctttt gccttactat tcattgtgta attccaatgt
    15661 cctaaaccat aaactaactt tgtcatttct tcatcactct ttttaaacat ctgcttttca
    15721 ttataaaaaa gcatataggc tggggtatta ataccttttg gacatacttt acaataacca
    15781 caacctgtac atatttcgtt catattctta tttaatctag tctcaatatc atgtatatcc
    15841 tcatctgaat aaattctatt ttcgtctgca attttacatg catcatctat atcttgtttt
    15901 ttattaaacc ctatcaaaga tatacttatt tgtgaaattc ctatgttgaa tcttaaagct
    15961 gattctatag tactttcgcc atcttttgat agaaaagaaa gttctttatt atactttgga
    16021 atagttccac cacttaatgg attcattgcc acaacaccat atccattatc aacagcatat
    16081 ttggcacctt ttagcctata tggaaagttt aatatattta ttcccatagt tacaccttca
    16141 aacttatttt cacttagaat atctataacc tcatctcctg gttgatgaga tgaaaatact
    16201 atatggtcta tcaatccttc ttctttacat ttcaataatc cttcatattg acctccaggt
    16261 ctcattgcta gttcgtaatg ttccatcttt ctaatattcc aaacatgata aaaatttatt
    16321 ttagatacgc ctaatcgttc tattgatttt tttacagcct ctatagcttt ttctgatgta
    16381 tcacatgctt ttggcttttc tttagttgat acataaaaat cggtcttacc ttcctttatc
    16441 atttgtctaa aggctagtcc aaatatatct tcacttctgt catcacaata ccctggcgct
    16501 gtatcaaagt aattaatacc tttttcatac gcatatttga ctaaatcagc attttcttga
    16561 ttactttttt ctaaatcaaa ccttaatcct ccatagccaa ctactgaaac cattttccct
    16621 gtctttccat attcaacgta tctcattgta aattcctcct tagatatatt aagacttaat
    16681 ttctttatag aaaatattat accataaata atttttataa attatttatg gtataatatt
    16741 ttttttataa attatatttt tttataactt gtattttata gctaagttaa aaactataaa
    16801 aaggttcaat atggactatt acttgagcat tttcattaat ttcttcttgt atttgtttct
    16861 caattttatg tgttaaatca tgagattgct ctacgcttat aaatgggtct accattacat
    16921 gcatgtcaat ataaatagaa cttttgctac ctctacttct gatattgtga acatccctta
    16981 cctcattaaa ttcaaacaca atttctttta tataatcctc atctacagcc actctatcga
    17041 ccaaaattcc aatagatggc ctaaatattc cataagccga ataaattatg aatgcagaaa
    17101 taataatagg aactatagat tctattatta ctggaaatcc taacttaaca ccaactaaag
    17161 taaccaatac acctagagac acaaatatat cacttcttgt atgcaatgaa tccgaaatta
    17221 aaacataact atttagttta gtcccaattc tatattcata catgcataca aaaatattta
    17281 taaaaagtgt aataattaaa gcaattaaac tttctatagt tatagcaggt acaactggat
    17341 tagctattct tagcactgca ctaagaatta tcttaattgc tataacaaac aacataccac
    17401 ttataaataa actggttata atttcaaact ttttatgtcc ataaggatga tttttatctt
    17461 ttggtttaga tgcaaagaaa atacctataa gccccactat attagatgct ccatctgaca
    17521 aagaatgaaa tccgtctgct gtcatactat agctttttat ttgattacca ataatgattt
    17581 ttaataatgc tactgcaaaa ttagcaaata aaattatcca caatacttgc ttcactttct
    17641 tatagttatc cataacaaat tccccctccc ttcgattcaa taaagaaagc tatctatcaa
    17701 tatcaatcac ccagataata aaatcgtata taagcacatt tactatattt atattcaaaa
    17761 ttggttgtac ttgttcattc tatttgaaac ttaattataa ataaaaatcg taggtatggt
    17821 tttggtatac ataaaaataa gagactgtct caaaataaag ataagcttct tttttattgt
    17881 tgaaatgata aaatatattt gatttcatat aaaaaagagt gcctcatgaa ctaaaaagtt
    17941 cattttgaga cactccctta aatttttgat ataaactatt aattttttat actaagcctt
    18001 ctttaactaa gaatgaatga gcaacttctt ctggtgattt accaagttca tccacttgat
    18061 agttaagttc tctcatagtt tcttcattta tttttcctga aagcatattc ataacttttt
    18121 ctaactcagg atatttcttc aaagtatctt ctcttactaa tggtgctgca taatatggta
    18181 caaagaaatt tttatcgtcc tctaatatct taagattaaa ctttttaata agaccatcag
    18241 ttaaaaatgc atctataacc tgagattcat tattatctaa agctgtatat cttaaacttc
    18301 cattcattgc ttttacatct ttgaatttta aaccataatc tctagaaagc cctacaagac
    18361 catcttctct attttcaaac tccaaagttg gtgagaatgt aaattcttta ccatatttag
    18421 tcaaatcaga aatagtattt attccatact tttcagcagt ttcaggcatc atagctaatg
    18481 tgtaagtatt attaaatcca agagggtcaa gtaaagttaa ctgattttct ttttccatag
    18541 tatcttttac aacattatat gcttcatcag catctttaat cggagcatgt ttcattacat
    18601 ttaccaaaag tgtacctgta tagtcaacat acatgtctaa ctcaccagat ttaattgcat
    18661 taaacgcaac agatgaacca cctagattta aattcttctt aactttaaga ttcgtatttt
    18721 tttctactaa ttcagcataa acattaccaa gtataagttg ttctgtataa tctttagaac
    18781 caatagttat tgtatctttt ttacttgaaa aactagaact aaaaacaacg aacaacattg
    18841 aaacaacaac cactacagat attactttaa gtgctatata atttttctta ggtgcttttg
    18901 gatttaatcc ttttggagta actgcaactt caattttact aaataaataa tcaactaaca
    18961 gtgcaattat acatgatggg attgcccctg ctaaaatcat attattattt acagtttgaa
    19021 cccctgaaaa tactaaatat cctaaaccac cagcacctat aaatgctgca agagtcatca
    19081 aaccaacagc tgtaactgca gatattctaa ctccacccat tattatagga agtgctaaag
    19141 ggaattgtat tttaaataat gtctgatttc ttgtaagacc aataccttta gctgattcta
    19201 atactactgg gtcaatactt gttataccta tatacgtatt ttttacaatt ggtagtaatg
    19261 aataaaccac taccataaat atggctggtt tacttcctat gccaagtata ggtacaagaa
    19321 gacccaaaag tgccatagac ggaactgctt ggactaagtt tacaaatcca agtacaaact
    19381 ttctaagaga tgagattctt gaaataatta ttccaagagg aacaccaact attatagcta
    19441 ttaaaataga tgtaacagtt agactcatat gttgtattaa aagctctata atcttatctt
    19501 tttgtaatag tataaagtta aaaaaattag ccaagaacac tcacctccat ttctaagaat
    19561 tgttcactta aaacagaaag taaactactt ctagtaataa gccctaccag tttattttca
    19621 tcagaaataa ccggaatata tccaaccgaa ttacgattca ttacatttag tatttctact
    19681 aaattatcac cttcatttac atgcaaaggc tcactcgaca taatatcagc aagtaatatt
    19741 gacttatcat caatatcttt catatctttt acagtaacaa taccttttaa tgtttttgct
    19801 ctatctataa ttagtaagct atcaacctta ctcgttctca taatttctat tccttgagtt
    19861 acagttctag tggaattaac agcaatagga ttttttatca taatatctct tgcttttata
    19921 tattctggat tatcccaaac tctatcttct ccgataaaat cttttacaaa gtcatttgca
    19981 ggctttctaa gtatattttc gggagtatca tattgtgcta ttcttccatc cttcataata
    20041 caaattttat cagctatctt taaagcctca tccatatcat gcgttacaaa tattatagtt
    20101 tttttcaatt catcttgaag agaaaataat tgttcttgta aagatgttct cgttattggg
    20161 tctaaagcac taaaaggctc atccatcaat attatttcag catcagtagc aatggctctt
    20221 gcaacaccaa ttctttgttg ctgtccacca ctaagttctg aaggatactt attcaaaaat
    20281 tcatcactat caagtcctac catctcaagt agttgcaacg ttcttttttc aatttcttca
    20341 acggattttt ccttttttag gcgtggaatt agctctatat tttcttttat tgtaagatga
    20401 gggaataggc ctgtgttctg tataacatat cctatttttc ttctaagctc tatttcattt
    20461 tcctgagaaa ttggtttgcc attaatatat atctcaccag atgtaggttt tattaatttg
    20521 tttataagct tcaaagtagt agtctttcca caaccacttg aaccaatcaa aactaccaat
    20581 gtacctgttt ctacaactag agaaatatca tctaatataa cattatttcc tatttttttt
    20641 gttacatttc ttatctctat cataatttat catccccttt ttcgaacaag caaccttctc
    20701 caatattata agttgttttt ataattatat catactattt tatttatgta aacaaagtac
    20761 gatttttagg tcactatttg tctgaatatt gctacaaata taaagtattt atatatagta
    20821 tataatgtca aaaaacaaaa agtataaaaa tattcataaa ataacactag cctcaattat
    20881 atagtattaa agctagtgtt atttcattta aacaaaactc acatttgaat actatccatg
    20941 tattcaaata atgtaatatc tttataagtt tcttttttat cacttaagtt tgatattgta
    21001 actcccaata gcctaatttg ttttccatta acctcaacat ttctcaacaa attatctaca
    21061 ccacttctta tatcattata ttcgtcaata tagtgttcta aactaaggct tctagttatc
    21121 tttgtaaagt cctcatattt aatttttaat gttaatgttt ttccaaactt ctcagagctt
    21181 tttaatctat gacatacttc ttcacaaagt tcatcaagaa tgttgcgtgt ttcttcttca
    21241 tcaatatcca aattatgact taatgtagtc tcagccccaa cagattttct aaccctattt
    21301 ggttccactg gtctatcgtc tattcctcta gcaaatttat ataattcata acctcttttt
    21361 ttgaatatat tttcaagttc aaataaattt aaacacctta aatcatatcc agtttttata
    21421 cctaaatttt taagagtatt tgaagtaact tttccaacac caaaaaattt attcacaggt
    21481 aatttatcta aaaagtcctg ggcattttct tcggttatta cagttaaccc atttggcttt
    21541 cttaagtctg atgcaatctt agcaagaaac ttattgtatg acacaccagc tgaagaagta
    21601 agaccaactt ctctaaatat atcttttttt atttgctttg ctatctcaat tgatgaatca
    21661 atattttttt tattttttgt tacatctaag taagcttcat ctaaagataa tggttcaact
    21721 aaatccgtgt atctataaaa tatatctctt atttgctctg acacactctt ataaacatca
    21781 aatctaggtc ttacaaaaac tgcatatgga cacctattgt aagctacagc tgatggcata
    21841 gctgaatgga taccaaattt tctagcttca taagaacacg ttgcaaccac acctcggctt
    21901 tgtggatttc caccgacaat aacaggtttt ccttttaatt ttttattatc tctttgttct
    21961 atagatgcat aaaaagcgtc catatctata tgaattattt ttctataatg tttcattatt
    22021 atcacctttt aaagtataaa tagattaatt ttatttatat tactttttaa ttataccaaa
    22081 cttatgtttc tttttggtat tttttcatta ttttctttat atattcatat tttccataaa
    22141 taaaaccatc tataatccat taacttcaat ataatttata aaaaatcctt aaaaacactt
    22201 gcaaaaacta tataaaagct ttacaatagg tattccaagg aactaaattt aaaattcctt
    22261 atggataaca gtatatatat ttaatttatg ggatgtttta aaaacaaaag actgtcttat
    22321 acaagacagc ctactgattt tattataatt gtaatggact gctcaaacta ttactttaaa
    22381 acagcccata tttttttata taattttaac tatagtaaag attttgtgaa ggatattctg
    22441 ggtcacaagt tacatctata ccaagctttc taaatgtttg ttcatcatct ctatttaata
    22501 tagtagtaga atgagcttga caccctttta ataatgctaa cttttccata gcaaactgtg
    22561 cagttggatt agttacagca caaatactaa gtgcagttaa cacctcttgg caactaagtg
    22621 aaacatttct actagctaaa gtctttgttt ttaagtttat gataggttct aatattacag
    22681 gtgatattaa gtgcatatca tcagatatat tggctaaatg tttaatggca tttaatacta
    22741 ctgctgctgt tccattcatt aaatcagaac ccttaccagt taatattgta ccatcttcta
    22801 attctaatgc aactacagga caagtatcat tcttgtcggc actttctttt aatttattac
    22861 tgtaatttct agcaggtaat acaacatttc tatcttctgg ctttaagtta agctcttcca
    22921 taatcatttt cattctatca taagcgcctt tatcaacttg tcccttttta tactcacagg
    22981 cagttttaaa gtatcttctt attatttctt ctatagaagc ttttcttatt acttcatcat
    23041 ctattattcc aaagccaact ctattaacac ccatatcagt tggagattgg aaaattgatt
    23101 cttttccagt tatcttttct attatttttt taagcactgg aaataattcc aaatctctat
    23161 tgtagtttac tgacatttca ccatacgttt caaggtggaa tgaatctatc atattcacat
    23221 cttttaaatc aactgtagct gcttcataag ctatattcaa aggatgtttt acaggtacat
    23281 tccaaactgg gaatgtctca aacttagaat atccaactac attacctctt ttattttcat
    23341 gatacaactg acttagacaa gtaccaagtt ttccactatt aggtcctggt gctgtgacta
    23401 ctactattgg ctttgtagtt tctatatatt catttgctcc atatccctca tcactaacta
    23461 tagtctcaac atctgacgga tatccctttg ttggataatg cttataaact ttaattcctc
    23521 tacgttctaa tttatttata aatacagttg tagcaggttg tccttcaaat ctagtaatta
    23581 ctacactatt tacatctagt tcataacctc ttaaatcatc tattaatctt aaaacttcca
    23641 tatcataagt gataccaaag tctcctctta ttttgtttct ttctatatct cctgcataga
    23701 cacaaattac aacttcaact ttttctttca tatgttgtaa tacttttatc tttgcatttt
    23761 catcaaatcc tggaagtact ctttttgcat gaaggtctcc catcaatttt cctccaaact
    23821 ctaaataaag tttgtcaaaa ttatttactc tttctaagat gtatttagac tgctcttcta
    23881 aatatttttt gtggtcaaat cctactttca tcttaacacc tcactaaatt aaatttcttg
    23941 ttgataacag tatatttata tatatttttt tagtaaaaca tcttataaaa aaatgttaat
    24001 actttttcac tcaaagtaat attatacaat taagattaat ttttttcaag atagttttat
    24061 ggtaaataag atatacctaa tataactaat aaaataagaa tattatattt ttatatataa
    24121 gtataatatt ctcaccttaa cattaattta tttcaatatt ttcatatatc ttttattcta
    24181 aattcaatac ttttctaata tcttctatac cttgataaac ttcttgttta gaaaaattca
    24241 tagcttctaa ttcttcatca gtcttttcca taagcatagt ataaaattct attgctatat
    24301 actgcatatt ttcactagtt acagtcttag ctttatcaaa taaaatttct tctgcttcgt
    24361 ttacatttcc ctgaaaaact aatcttttta aagttataag tataacttct tcttctgtat
    24421 tatacactaa atctttttct tcatattcac tgtatttttt tcccaataaa agtcttgcaa
    24481 ccataagttt tatctcattg aaaatcttat ccactatagc catttttaca tctcctataa
    24541 ataaatttta ttttcttttt aattatatca gactttattg taaaaattat tatatgtaaa
    24601 tgaaataatt caaaaataaa ttctaacaat aaaaaaaaga aggtagcggg ggatttccga
    24661 ctaccttcaa acactagatt gtgattggtt tggtatttta tttataccca ccataagctt
    24721 tattaaatca tctaaattga tttttttagc atccacatcc tccatcaaaa acgtcaccac
    24781 attcacaaag tattaataat aatattaaga aaggtatcca tgcacataag tccatttcag
    24841 cccaacaatc ttggaatgct aagaataaga agaataatat tattatccac caagcacatc
    24901 caccaaatcc gccttcacca aaaaatctat ccaacataat atgttacctc ctagtcattt
    24961 tgtatgtgga gtttcagctc ctaaaatata ctatgtttaa ggggtaatat ttgctaatga
    25021 tttttttaaa ttttttttag atattttaaa aaaaattatt gattcacact caatgtctta
    25081 ccatcactat tgataactac ctctccacta gaagtcaaaa atgtttttat attcaatctt
    25141 tccaatattt ttaaaacacc ttcctctggt tcttttttct ctgaacatgt aataacagca
    25201 tattggggtg aagtagactc taaaaatgtt tcagttaatt tgtcatatct tccatgatga
    25261 ggcattttaa caaaatcata ttttaaagta tttccattta taaactctga caatctttct
    25321 tcctctgcat ctcctgcaaa taaaaactta tgtgctccat agcttatatt agtgattatt
    25381 gaataatcat ttgaactctc gtacttgctt tttgaagccg gatgtatgtt tatttctgca
    25441 ctattgatta caatattcat attatctttt agtagcactg gttctatatc agctttttta
    25501 agtgagtcaa tatattcatc atattgctta ctatctttct tataatttgc ttgtataata
    25561 ttctttactt taacactaga taacacacta tctactccac ctatatggtc cttatctaaa
    25621 tgggttaaac tgaacctcct atgactaaag tcacagggtt cctgcttcat agaattttgc
    25681 atactaacat atgtcttact aattctccac aggctatccc cgtagttcct acggttctta
    25741 catacgttat ttattttgaa ttgttattag tcttaatcct tcttttaata tatttatact
    25801 tgcatttata tctctatcgt gagtttcatt acaactagga caaatccatt ctcttatatt
    25861 taagttctta acttcctcat ttttataccc acatttattg catatttgtg aacttgcaaa
    25921 gaatttgcct acttttacaa tttgtctacc atgccaatta gccttgtatt ctaattgacg
    25981 aacaaattct gaccatgaca catcagaaat taagcgagat agtttacgat ttttaatcat
    26041 attctttact tgt
//
LOCUS       DAMKPG010000053        25972 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMKPG010000053 DAMKPG010000000
VERSION     DAMKPG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25972
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.056"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            219..3767
                     /locus_tag="PC621_002245"
     CDS             219..3767
                     /locus_tag="PC621_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF4109395.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKIIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            3952..5886
                     /locus_tag="PC621_002246"
     CDS             3952..5886
                     /locus_tag="PC621_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF4109396.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5992..6987
                     /locus_tag="PC621_002247"
     CDS             5992..6987
                     /locus_tag="PC621_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4109397.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6959..7744
                     /locus_tag="PC621_002248"
     CDS             6959..7744
                     /locus_tag="PC621_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF4109398.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7732..8430
                     /locus_tag="PC621_002249"
     CDS             7732..8430
                     /locus_tag="PC621_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109399.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8553..9026
                     /gene="ybaK"
                     /locus_tag="PC621_002250"
     CDS             8553..9026
                     /gene="ybaK"
                     /locus_tag="PC621_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF4109400.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9223..10062
                     /locus_tag="PC621_002251"
     CDS             9223..10062
                     /locus_tag="PC621_002251"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF4109401.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10075..10623
                     /locus_tag="PC621_002252"
     CDS             10075..10623
                     /locus_tag="PC621_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF4109402.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10689..11870
                     /locus_tag="PC621_002253"
     CDS             10689..11870
                     /locus_tag="PC621_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF4109403.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12043..12306
                     /locus_tag="PC621_002254"
     CDS             12043..12306
                     /locus_tag="PC621_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF4109404.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            12832..13515
                     /locus_tag="PC621_002255"
     CDS             12832..13515
                     /locus_tag="PC621_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109405.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13526..14767
                     /locus_tag="PC621_002256"
     CDS             13526..14767
                     /locus_tag="PC621_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109406.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            14839..15522
                     /locus_tag="PC621_002257"
     CDS             14839..15522
                     /locus_tag="PC621_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109407.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15519..17849
                     /locus_tag="PC621_002258"
     CDS             15519..17849
                     /locus_tag="PC621_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4109408.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(18030..18872)
                     /locus_tag="PC621_002259"
     CDS             complement(18030..18872)
                     /locus_tag="PC621_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF4109409.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19167..19901
                     /locus_tag="PC621_002260"
     CDS             19167..19901
                     /locus_tag="PC621_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4109410.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            19911..21044
                     /gene="nagA"
                     /locus_tag="PC621_002261"
     CDS             19911..21044
                     /gene="nagA"
                     /locus_tag="PC621_002261"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF4109411.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            21143..21892
                     /gene="nagB"
                     /locus_tag="PC621_002262"
     CDS             21143..21892
                     /gene="nagB"
                     /locus_tag="PC621_002262"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF4109412.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22082..22339
                     /locus_tag="PC621_002263"
     CDS             22082..22339
                     /locus_tag="PC621_002263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109413.1"
                     /translation="MFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQRRSNLG
                     ISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            22999..23703
                     /locus_tag="PC621_002264"
     CDS             22999..23703
                     /locus_tag="PC621_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109414.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            23705..25192
                     /locus_tag="PC621_002265"
     CDS             23705..25192
                     /locus_tag="PC621_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109415.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25397..>25972
                     /locus_tag="PC621_002266"
     CDS             25397..>25972
                     /locus_tag="PC621_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4109416.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFL"
BASE COUNT        10368 a         2676 c         4520 g         8408 t
ORIGIN      
        1 ataaatcaaa aataaagtta tagtttttaa tattaattaa gaatgatgaa atatgaataa
       61 agattttaca aataaatctt aagctatctt atttaattct aatgcaggaa gaaggtaaat
      121 gtatattgaa aaaatcatag aaatagaagt aaacaactta aacaaataca aaggtttgca
      181 aaagaagtaa tataattata tacaatgagg atgtgagtat gatgaacaga ctaaagccaa
      241 gaattttagg tattagtatc ttgtttatta ttataattat tttaacatgt ggagctgttt
      301 tcaagtttaa atttaaatta gataataaat taaaagaaac cacacataca tatttgtctg
      361 agtttacaga acacaatata attaatctca agacaagact tagtggtcaa tttgacatgc
      421 ttgaatctat tgctaacttc ataggaaact tgaataatat aaataatgaa atggtgattg
      481 ctttaatgga ttctgatgta aagcgaagtt ctttaattag aatgactatc tctacattag
      541 atggggaatg ttattctaat gatggaatca cccataatgt tcaagataga gattatttta
      601 aaaaagctaa aaatggagaa aagaatattt cagagccttt aaaatctatt gttaataata
      661 aagaaataat agttttatct accccagttt ataaaagtgg gaaaatagtt ggtgttttaa
      721 gtggaattta tgattcaaaa aagttaaatg agttattagg attatctgca tttgatgaaa
      781 aagcagatgt ttttgttgca aagaaaaatg gagatatagt tgctcaaaaa acaaataaaa
      841 atagaaagac gatatcatgt atatttcagc tttttgacaa agataattgt gatgaagatg
      901 attgtctaaa aactataaaa gaaaatatga aaaatgataa gtctggatat tttaattata
      961 acttagataa tgaaaaattt atgttaaact atcagccact tggagttaat gattggtatg
     1021 ttttttctgt tgtgccaaat aaaatagtgt ctattcagtc agagaatata tcttatgatg
     1081 tatatatttt tgcattagaa atactactag ttatattgat ttttataata tatatagcat
     1141 gtattataaa taataacacc aaaattatta taaatgaaaa agaaaaattt aaagtactca
     1201 ctgataatat attaggaggg gttaaaacct gtttaaaaga tgaatatatg actatgacat
     1261 atgtaagtca ggggttttta gagcttacag gttactcttg tgaagattta aataaaatat
     1321 ttaaaaatag atttaaagac atgatttata aagatgattt agacaagttt aattgtatta
     1381 ttctaaatca attagaaaaa caagataaaa ttgaagttga atatagaatt gtaagaaaag
     1441 atggaagtat aatttggatt ttggagagaa gtaagtctat aaatgataat aaaggaagtg
     1501 agtgtattca gggagtttta actgatataa cagaacttaa gaaaatacaa gaagagttag
     1561 attcaaaaag atttgaaata caggctatca atgatagtat tgttggtgga atagtgataa
     1621 ctgagataaa tgatgatttt gatagtatat atttaaatga aggttattta aatatgattg
     1681 gatatacacg caaacaactg gaagatgagc taaataataa attaatcaaa cttattttta
     1741 aagatgatag aacagaatta attaatagta taaacaaaca actcaaatta agtgatacat
     1801 ttgcatctga acaaagagtt gaaaaaaggg atggtactat tatatggatt ttattaaaag
     1861 gaagaaagat aatttatgat aatggaaaat ctgtactttg ttgtgttatt gtagatatca
     1921 cttctgctaa attattagag caagaactta aaaagagtga agaacgtcat gaaatcattc
     1981 taaagcagac gcaaaatatc ataatagaat gggatataga aaaagataaa atgaatatat
     2041 cacctaattg gtataataag tttaaatatg accctataac tgaaaacgca acaaaaaata
     2101 taattaaatc agtagatata tatcatgatg atatggataa gtttatttca ttaattaaac
     2161 ttacttcaca aggtagtgag tatgaagagt gtgaaattag acttagaact agagatacta
     2221 tttacgcttg gtttagtata aaggcaacaa ctttatttga taataatggt aaaccttata
     2281 aagtagtagg aagtttgact gatattgatg aatcaaaacg agaagctgaa aagttgaaag
     2341 aaagggcaga aaaagaccct cttacagatt tgtataataa aaaagcatca caatcactta
     2401 tagaagagta cataacattg gataaaaaaa gaaaaggtgc tttaatgatt attgatattg
     2461 ataactttaa aggaataaat gataatttag gacatttata tggggatgct gtattaagtg
     2521 aaattgcaaa tgacctaata aatctattta gagcaaccga tatagtaggc agaattggtg
     2581 gagatgagtt tattgtattt ataaaagata tttctgaaac aagagatatt ataaataaag
     2641 cagaagaatt ggtaaaaaca tttgaaaggt cttttttagg taaaggacga aattataaaa
     2701 tatcattaag tgtaggaatt gctaagtttc cacaagatgg tatggactat atgacactat
     2761 ttagaaatgc ggatatggct ctttactcag ccaaagggaa gggtaagaat tgttatacat
     2821 tgtatgataa aaaaattgat aaagtccaat atataactaa tacaagaaat gcaggagata
     2881 gtgataatgt agagatgtct tttaaagaac atatcacaaa ttatatattt gatatactat
     2941 atgaatcaag tgatatagaa gtggcagtaa atttaattct aagtattgta ggtaaacact
     3001 ttaatgtgag tcgcatgtat atatttgaaa attcagatga taatcgagta tgtaaaaata
     3061 catttgaatg gtgtaacgaa ggtgtaacct cggaaattaa taatcttaaa aatatttgtt
     3121 acaaggattt aggcaactat cttttaaatt ttaatgcaga tgggttattt tattgtaatg
     3181 atattgaaaa tttaggtagt gatgtgaaaa aagtacttaa gccacaaggc atacgttcaa
     3241 tattacaatg taaaatttta gagggcaatg aatttagagg gttcatagga tttgatgaat
     3301 gttcatataa tcgtatgtgg actaatgatg aagtgaacac tttgacattt atatctaaaa
     3361 cgctcagtat gtttttatta aaaatgcgta ttcagagtaa gctagaagag tctttaacaa
     3421 ttcaaaaatc tgtgatggat aatatggata tatgggcata tgttgttgac aggaatacct
     3481 atgaattatt atttatcaat aaaaaaatgc ttgagtcagc atcaaacact catgtaggtg
     3541 atttatgtta taaagcgata tggaataatc aagataaacc ttgtaaaaaa tgccctatga
     3601 tatatttaaa agatggtgat gatacttata aatcatttat gcgcaataaa aagtttgagg
     3661 tacaggcaaa tataactaca actactttga aatggtttga tgggaaagat gcatgtttaa
     3721 tatttggaag tgaagtaact agtattgaag ctgaagaaaa tacttaaaat actgtgttat
     3781 atatatactt aaaatcgcaa tttgaattta ttagacttaa aatgtctata taaatcattt
     3841 aagttgtggt tttaagtatt ttttatgata taattaagcg ttaagaacaa aattaaaaga
     3901 tacttttgta taaaaaaata tttgttaaat atatattgag caggtgataa tatggaaaat
     3961 aagtttttac caataagcaa acaagatatg atagatagag gatgggaaga attagatttt
     4021 gttctagtta caggagatgc ttatgtggac catcatagtt ttggaacagc aataatatct
     4081 agagttttag aaaatgctgg gtataaagtt ggaataatag ctcaaccaga ctggagaact
     4141 acagatgatt ttatgaagct aggaaaaccg agacttgcat ttctagttaa tgcaggaaat
     4201 atggattcaa tggtcaatca ttattctgta agtaaaaagc atagagaaaa ggatatgtat
     4261 tctccaggtg ggaaaatggg ttatagacca gatagagcta ctatagtata ttgcaataaa
     4321 ataagagaag catacagtga tgttgcaata gtaataggtg gaattgaagc gagtttaaga
     4381 agatttgcac actatgatta ttggagtgat aaggttagaa agtctatgtt aatagatagt
     4441 ggagcagatt tattagttta tggtatgagt gaaaagcaga ttgtagaagt agccaatgca
     4501 cttaatgatg gatatgaccc aaaatatatt agacatattg atgggacatg ctatatttca
     4561 gatacattgg aagaaatata tgataaatat atattaattc catcatataa agaaatttgt
     4621 gaagataaaa tgaagtatgt agaagctttt aaaattcaat atgatgagca agacccattt
     4681 agaggtaata taattgttca acctcatgga agtaagtatc tggttcaaaa taagccagaa
     4741 aaaccactta gtagagaaga actagatgaa gtatatggat taccatatca gaagacatac
     4801 catcctgtgt atgaaaaatt tggaggaata ccagctatag aagaagtaaa atttagtata
     4861 gtaagttcaa gaggctgttt tggtagttgt tcattttgtg caataacatt tcatcaaggt
     4921 agagcagtgc aaagtagaag tgaaaaatca attattgatg aagcaatagg aataacaaat
     4981 ttagatgatt ttaaagggta tatacatgat gttggaggtc caacagctaa ttttagaaga
     5041 ccagcatgta aaaaacaaat tactaaaggt gcttgtaaaa atagacaatg cttatctcca
     5101 tcaccatgta aaaatttaga tgcagaccac agtgaatact tacatttact tagagctgta
     5161 agaaaattac ctaaaataaa aaaggtgttt gtgcgttctg gaattagata cgattatgta
     5221 atggcagata agaacaataa atttcttaga gagttgatag agcatcatgt aagtggtcag
     5281 ttaaaggtag ccccagaaca tatatcagaa gaagttttaa agtatatgca aaaaccagca
     5341 ggaaaaactt atgataaatt tagacagaag ttttttgcta taaatgaaga attaggtaaa
     5401 aagcaatatt tgatacccta tttaatgtct agtcaccctg gttctacctt aaatagcgct
     5461 atagagttag cagagtattt aagagatact cactaccaac cagagcaggt tcaggatttt
     5521 tatccaactc cagggactct atctacaacc atgttttata caggtattga cccgcttaca
     5581 atgaagccag tttatgttcc taaatcaaaa cgtgacaagg caatgcaaag ggctttatta
     5641 caatatagag cacctagaaa ttatgatttg gtatactctg cgttagttga agcaggaaga
     5701 gaagacttaa ttggttttgg acatagatgt cttataaagc caaaaaatga aaagccttat
     5761 tttaatagaa ataattctaa aaaaaatgta tctaaaggaa caaataaaaa caaaaaaact
     5821 aatacaaata acagaagtaa taaaaatcaa caaaaaagtt caactaaaaa aaagaaaaaa
     5881 cattaaagta taaataaaaa tacttaaaaa agaaggatta ttcgataaat taacagaaat
     5941 tagtataaat aaatataatt attttaatga cgttaccttt gggaggaaga gatgaaaaag
     6001 tatatttcta taatattatt agtggtggtg actatggttg ttttggtagg atgttcacca
     6061 ggaaaagata accctaaaaa taaggagcta tcagttgtca aacaagtaaa agtagctgtt
     6121 ccagatggac taccagcagt ggcaatagct aaacttgcaa atgaaaatcc agagattaaa
     6181 gaaggatatg aaacagtgta tagcattgaa aagacacctg aggcgatttc cactagagtg
     6241 atgaaaaaag atgcagatat agctatagtt ccttcaaaca tggctgcaat agcgtataat
     6301 aaaacttcat catataatat tgttggaact gtaggaatgg gttcacttta tttggtgtca
     6361 acagaaaata taaaagatta ttctgattta gttggtaaag aagttggatg tactggaaaa
     6421 ggacttactc cagacataac tataaaaagt cttttgttac aaaaggatat aaattattca
     6481 aatataaaat ttaattatgt aaattcagca agtgaactgg taccattatt ggctactggt
     6541 aaagttaaga caggtattgt tccagaacct gcacttagtg ctttgatgtc taaaaatcca
     6601 gacataaaaa ttataaaaag tttaaatgac tcatggaagg aagtaagtgg ttcaaaagac
     6661 ggttatcctc aatctacttt aattgtaaag tctgaatttt taagagataa taaagatttt
     6721 gttgattctt ttgtaggtca attatcaaat agtatagatt gggcaaacaa aaatcctgaa
     6781 gaattaggtg catattctga aaaaataaaa atatctactg aatcaaagat aataggaaaa
     6841 tctcttgaaa gagctaattt aaaatatatt ccagttaagg acatgattaa agattataaa
     6901 aattactatg agaaattagc taattttgat gataagacat taggaggcaa agtaccagat
     6961 gaagcaatat actttgttga gaaataaaaa aaataaagaa caatttatag ataaaatgga
     7021 ggtaacactt tcttgtgtta tcctcttatt tttatggcaa attattgctt taaaaattga
     7081 taatgatatt tttttaccaa cagtaggtca agtatttaat agtataaaag agattatgct
     7141 aaattctagt ttctatatag atatacttta ctctatgggg agatgttttt ttagtttttt
     7201 actggctatg atttttgcca ttatactttc aatagtatct tatttaaata gattttttag
     7261 aaacttatta aagccaatca atgctttgac acgttcaata ccaactatga tattggtagt
     7321 acttgcactt atttggtttg aaaaggatag tacccctttt atagttggat ttactatagt
     7381 ttttcctata ttgtatgata acgttttggg cgctatattg aatatagaca agaacttatt
     7441 agaaatggct aatgtgtata aaataaggtt tatagataag gtgttaaaaa tttatttacc
     7501 agccataaaa tttcaaatta tatctatact agtatcaaca ttctctttaa gtttgaaagt
     7561 tgctatagca ggagaggtat atagtcaacc tacatatggt attggaacta tgatacagac
     7621 tgaaaaaatt aactttaata catctggaat atttgcatgg ataattattg ttcttttaat
     7681 atctgctatt ttacaaatag cccaaaagtt tttagcaagg agagcttttt tatggaaaag
     7741 ataaagttga gtattgaaaa tataaataaa agatatgata gtcgtattat atttagagat
     7801 tttaatattg atttttatgt aaatgaggta aattgtatcc ttggaaaatc tggttgtgga
     7861 aaaactacac tcttgaacat aataagtggg attattaaaa atgacaccaa caatctaaat
     7921 atcaaagaaa atttaaatag agtaggaaat aagcttgaag ctagttacat atttcaagat
     7981 gatagactta tagactggtt aacagtagaa gaaaatataa agatagttgt aaataaatac
     8041 tataataaaa ctcaattaaa taaaatttgt gatgaatact tggaattggt tggaattagt
     8101 gattataaaa aattctatcc tcagatgctt agtggaggta ttagacaaag agtaaatatt
     8161 gctagagctt ttatatatcc atcaaaaaac attataatgg atgaaccttt taaatctatt
     8221 gatgcaaaga atacacagct tataatggat aatttcagaa atatattaag aaaagaaaag
     8281 agaactgtat tatttgtaac tcataatatt gaggaagcat tgtttttagc agataggata
     8341 tttattttag gagactctcc aattagaata aaaaaaatat taaaaaattc taaggaattg
     8401 gaaaaaaatg aagttttgaa gttgatatag agttataata atactgtcgt tttaaaatat
     8461 atttggttaa aatctataag actatattaa aatagtacag aatattcaaa actgtgtata
     8521 atgttcgtat tttataaaaa ggaagtgaat gtatggttaa gactaatgct atgagaatat
     8581 tagattcaaa taagattgat tataaagtaa tgtcttatga agtgaaaagt gaacatgtgg
     8641 atggtgttga ggtggcacat gatataggaa gagatgtaaa tgaagtgtat aagacattag
     8701 taactcaagg agttagtaag aatatatatg tatatgtaat accagtacat gaaaacttag
     8761 acttaaaaaa ggctgcaaaa gtagctaaag agaaaagtgt agagatgatt catgtaaaag
     8821 atataaataa gcttacaggt tatattagag gcggatgttc tcctataggt atgaaaaagc
     8881 tttataaaac ttttgtaaat gaaagtgcaa agaatttaga tacaattatt gtgagtgcag
     8941 gaaaaatagg atatcaaata gaactatctc catttgattt acaaagactt ataaaagtag
     9001 aatttgtaga tgtaataaaa aaataagaat tacatataaa atatagagct tatattctta
     9061 ataataggtg ttgtaatatt aataaaatga ataataattt taaaataaat attcagttac
     9121 ttatgtgtaa ttggtataaa aatattgaga aattttaaaa acaatggtaa agtgtaagta
     9181 taatcacgaa tattttttat aaagtaatgg agggatgttc gcttgagaaa aataaaatct
     9241 gaacaaatcg tcgagcaggt aaaaaaactt tgtatagaag cgagcctata tttaggagaa
     9301 gatgttttaa gttgtataaa agaaaaagcg aaaagcgaaa agagtgaagt tggaaaaaat
     9361 atattgaata tattagttga aaatgctgaa atagctaaag aaaaaaatat accaatatgc
     9421 caagatactg gtatggctgt attttttgtt gaaataggac aagaagtttt aattgaagga
     9481 gacacactta ctgatgctat aaatgaggga gtaagacaag ggtatgaaga aggctactta
     9541 agaaaatctg ttgtaagtcc aattaacaga gtaaatacaa aagataatac acctgctgtt
     9601 attcattatg atatggtaaa aggtgataaa attaaaatag aatttgcagc taagggattt
     9661 ggaagcgaaa atatgagtaa gatgaaaatg ttaaagccat cagatggatt ggaaggaatt
     9721 aaaaaattca taatagatac agtttcagaa gctggaccta acccatgccc tcctatggta
     9781 attggagttg gtataggtgg gactgtggac aaatgtgctc aaatagctaa aaaggcactt
     9841 tttagagaac taggtgaatt taataaggac gaaaatatag caaaacttga aagtgaacta
     9901 ttaacagcta ttaataaact tggtatagga cctcaaggct taggtggtac tacaacagct
     9961 ttgggattaa acatagagac ttttcctaca cacatagcag gtctacctgt tgttgtaaat
    10021 ataaactgcc atgcatcaag acataagaag gtagtaattt aggaggaaaa taaaatgata
    10081 aaaataacta cacctgtaaa tgaaattgat atagcaaaat taaattgtgg agatacaatt
    10141 agtttgagtg gtatacttta tacagcaaga gatgctgctc ataaaagatt gatagattgt
    10201 ataaataaag gtgaagaatt accatttgac gtatatggtc aaggaatata ttatgtagga
    10261 cctactccta caaagccagg agaagttata ggtgctgcag gtccaacgac gagttataga
    10321 atggatgatt tgactatacc tcttttagaa agaggtctta gattgatgat aggcaaggga
    10381 aaaagaagtg atgaagttat agaaggtatg caaaaatacg gagcagttta tcttgctgca
    10441 ataggaggag ctggagcgta tatttcaaat tcaataaaat catgtgaaat tattgcatat
    10501 gaagatttag gagctgaagc tataagaaag attgtagtag aagaccttaa acttacagta
    10561 gcaatagata gctatggaaa taatatatat gagcaaggtc gagcaattta tgaatgtaaa
    10621 taatgtttag aaataaatat gaatatcaaa attaaaaata gttgtgtata ttttaaggag
    10681 gaaaattgat gtctaaggat tatgcaaaat tggcattaga aatgcatgaa atcaacaaag
    10741 ggaaagtaag tgtagaaagc aaggtagaag ttaaaactaa ggatgattta tcaacagcat
    10801 atacaccagg ggttgctgaa ccttgtttaa aaatacatga aaatcaagat gatgtttata
    10861 tatacacttc taaagggaat ctagtagcag tagttagtga tggaagtgct gtacttggat
    10921 taggtaacat tggtgctgaa gcatctatac ctgttatgga aggtaaagct atattattta
    10981 aacaatttgc agatgtagac gcttttccaa tatgtttaaa gactaatgat gtagatgaga
    11041 tagttaaatc tgtagagttg atggagcctg tatttggagg tataaatttg gaagacatat
    11101 cttctccaag atgttttgag atagaagaga gattaaaaaa atctttaagt atacctgttt
    11161 ttcatgatga ccaacatggg acagcaataa tagttgcagc agccataata aactcaataa
    11221 agttaattga aaataaaaaa atagaagatt tagaaatagt tataaacgga gcaggagctg
    11281 ctggtatagc catagcaaag atactactta atatgaatgt taaaaatata atcttatgtg
    11341 atagaagtgg agcattggaa gctagtatag aaaatctaaa ctatgttcaa aaagaaatgt
    11401 taaaagtaac aaatataaga aatgaaaaag gacctttaaa agatattata aaaggaaaag
    11461 atgtatttat tggtgtttca ggtccaggag ctgttacaaa ggaaatggtt gaaagtatgt
    11521 ctgagaagcc aataatattg gcaatggcaa atcctactcc agagataatg ccagaagaag
    11581 caaaattagg tggagcattt ataatgggaa caggtcgttc agacttccct aatcaagtca
    11641 ataatgtact ggcttttcct ggaatattta gaggagcatt agatgttaag gctaaagaga
    11701 taaatgaaga aatgaagata gctgctgcat atgcaattgc taatacaata agtgatgaag
    11761 aaatatgccc agagtatata ttaccagatg tatttaataa gaatgtagta aaaaatgttg
    11821 ctaaggcagt taaagaagca gcgataaaaa ctaaagtaaa tagaatataa atttttataa
    11881 cttattaaaa actagaaaag tataaaagac tagtgaaatt atgtacagtg ggatgatttt
    11941 attagtcttt ttattttaaa tatgacacaa taatgtcata ttaagcttga tataatttta
    12001 atatggttag gagccaaaga aaaatataag gagtgattta aaatggaaga aactaaattt
    12061 tgccaatgct gtgcaatgcc aatgggacaa actgatgaat tatatgggac aaataaagat
    12121 ggaagtaaaa gtactgatta ttgtagttat tgttttaaag atggtgagtt tacagctgat
    12181 ataagtatgg aagagatgat agaagtttgt atccctcata tgcttcaaag taataaggat
    12241 atgactgaag atgaagcaag aacaatgatg aacaatttct ttccaacatt aaaacgctgg
    12301 aaataatttt atgcaaaatt agttatatgc aaaacatgaa atatggtgtt gagaataagg
    12361 ggtggtgtgc tttatagcat accacctgtt ttgttacttg ctagttcagt tattcttgta
    12421 tgcttaaata tgttctcatc tctctttcta ttttaaattg taaaaatcta aaatatgtta
    12481 tttatattct acttctttat ccatgtcttt ttctacctca agtcaaatac aactacaaaa
    12541 tcatcatttt caattcactg tttaatagaa ttaacagata aagagttata aaattttcta
    12601 ttttttatat aaaataatac aggtcaagct gacttctagc tacttgggat agtgtatata
    12661 tttatgatag ttataggagt aataccaatc attagactgt tgatttgtgt agtcttttgt
    12721 aaaaacattt gttttaatat attgagtttt tgaaaaagta aaattataat tataataaat
    12781 taagtgtaat aatgtattag aagtaaaaat aattaaaata gaggtgatta aatgagcagt
    12841 atattgttag ttgaagatga tttaagcttg atatatggat tagagttctc tatacaaaaa
    12901 agtggattta gtatagacat agcaaggaca gtaaaagaag ctatgcaaat gtatgaggaa
    12961 aaaaattatg acttattgct tttagatgta tccctacctg atggtaatgg atttcagatt
    13021 tgcgaaaaag tacgagaggt ttctaatata cccattatat ttttgactgc ttcagatgaa
    13081 gaggtaaatg tagtgatggg tcttgatatg ggtggcgacg attatataac aaaacctttt
    13141 aaattaaatg aattaatttc aagaataaaa gcattactta gacgttataa tgttgcaagt
    13201 aatgtgaatg aattaagttc aaataatatt actattaaat tacttgaaaa tcgtgttttt
    13261 aaaggtgaat ttgaggtgga acttactaca gcagaatata aattattgtg cttatttatg
    13321 aaaaataaaa atattgtact tactagaaaa aacatattgg acaagttatg ggatggaaat
    13381 ggtagcttta tagatgacaa tacattatca gtatatgtaa gacgattgag aaataaaatt
    13441 gaagataatc ctgaaaatcc taagtttcta ttgaccgtta gaagaatggg ttataagtgg
    13501 aatgtaatta agtgagggta ttaatatgaa gatattttct aataaagata taaaaatatt
    13561 ttttttaatt cttatatcta ttttagtaat gtttattata gtagggcaaa tagtggttat
    13621 aagtataact aacaattata aaaaaacgtt gatagaacat gattataata tagctggata
    13681 cttatatagt ataggtgttg ataaatctaa aataccatca gcatttgttg cacatgaaga
    13741 taaatatatt ttaaaaggaa aagaaatact agatacagct ggatatggat ttgatataga
    13801 tgttagcttt acaccaagtg taaaattgtt tcaaagtaaa tatataaaaa ctatgtctat
    13861 ctatttatta gtatttttta ctatgattat tttcatacta tatatgtatt tttttaggca
    13921 acaaagtaag atagaagagg ctagttataa aattgattta tttatgaatg gaaatataaa
    13981 tgaaagacta aatacatatg aagaaggtag cttatcaaaa ttatttagtt caatagatgt
    14041 tatgtcgaca tctttaaata cacatatatt aaaagaaaaa cagagcagag aatttcttaa
    14101 aaatacaatt tcggatatat cgcatcaatt aaaaactcca cttacagctt taataatgta
    14161 caatcagata ctacaagaag aaagtcataa tagtgatgtc gttgaagctt ttgtacaaaa
    14221 aagtcaaagt gaactagaac gcattgaaac attgattaag aatttactaa agattacaaa
    14281 gatagattca aaaacaatag ttctcaataa aaataatgtt aatcttaaaa atttaattgt
    14341 caaaattttg tatagctttg aaactagggc agaaaaagaa ggaaaggtta tttctataaa
    14401 tggttcagaa aatacattgt tgttctgcga ttatgaatgg atgtcagagg ctttaagtaa
    14461 tcttataaag aatgcattag ataatacaaa agagaatgat aaaataacga tagagtggtt
    14521 tgagacgact ataaccacca ttgtttcagt taaagacact ggaaatggaa tccatgttga
    14581 ggatattcat catatattta aaaggtttta tagaagtaag ttttcaaaag ataatcaagg
    14641 tttaggactg ggattaccac tagttaaatc tattgtggaa caacataatg gtactatttc
    14701 tgtagaaagt aaactatcac aaggaagtac attcaccata agttttttaa agcttactaa
    14761 tatgtaagat aaaatacatc ttgcagtaag gtatatagat tattattata ttaatcaatt
    14821 agaaaggtga gagagaaaat ggatattctt gtggttaata atttatgtaa aacatatgga
    14881 aatggatata caaaagtaga ggcattgaaa gatgtatcct ttacagtttc aaaaggagag
    14941 tttgtgactg tagttggaga atctggttca ggaaagagta cactattaaa tctcttagga
    15001 ggtcttgata ctccaacatc agggagaata tttattgatg gtaaagaaat atgttcaatg
    15061 aaagaggaaa aacagactat ttttagacgt cgtaacatag ggtttatatt tcaggcatat
    15121 aaccttatac cagaactaac tgtggagcaa aatataatat tcccattgtt attagattat
    15181 aaaaaaccag atactattta cttagatgaa gtattgacta ttttaggtct taaagatagg
    15241 aaaaatcatc ttccaaatca attatctggt ggtcaacaac agagagttgc aattggaagg
    15301 gctttgatta caaagccaag tattatactg gcagatgagc caacgggaaa tcttgacagt
    15361 aaaaatagta atgatgtaat ttcactttta aaagtgactg cagaaaagta taatcaaacc
    15421 attattatga taactcataa tacgaatata gcttctacag cagatagaat actacaggtt
    15481 tctgatggtg tgcttacaga cctgggggtg aatagagaat gagaagctac ttagaattag
    15541 taccacaata tgcaaaagta cataagaaaa aaaatagaac tacaatttta tgtattatta
    15601 ttgcagtatg cttagttact gctatctttg gacttgcaga tatggctata caatgtcaaa
    15661 agatacaatt tattaaagat gatggaaatt ggcacatagt aattagaaat attgatgaaa
    15721 agtttatatc agaaataaat agtagagtag atatagctca ccatggtagg gggcaagtcg
    15781 ttgacagtgg agagtttaaa tcaaaggaga ttttgataca aggtttagat gaagaagtag
    15841 caaaacaatt taacttagaa ataataaggg gtcgttatcc tattggggag aatgagactt
    15901 taatagatga ggatgcagtt aaaagcttta atctaaaaat aggagatttt atcaaaataa
    15961 aattagcaga tggtagaaat aaaaattata agatagtagg tgaatattca tcaatatcaa
    16021 gcttaagagc tacagataaa catggattaa tactttcaat attgggtaca caagctatta
    16081 aaaaatcaga aacgtacgat tattattata ttacatttaa agaaaaggtt aatattagaa
    16141 aagccacttc tgatattaaa aataccttca atttaagtga taaacaaatc ggtaaaaatg
    16201 aaagattact ttctattata ggtcaaggcg actctgactt ttctaaacca ctgtatttaa
    16261 cagcaggatt actttttgta cttgttttag tagcaagtat tatcatgata tataatagtt
    16321 ttaacatgag tgtaattgag agagttaaat tttttggtct gttacgttgt ctagggtcat
    16381 caaaacatca agttaaaaaa tttgttgttt tagaaagttt aattttaagt ataaaagcta
    16441 ttccaatagg cttagtatta ggaagtcttg taactattat atcttctata tttcttaagt
    16501 atgttaatga gttattcagc tcaatgccaa ttttaaaagt tagttttatt ggtattgcat
    16561 ttggaataat tgtagggttt ttaactgtaa tattatcagc tattattcca gcaaaaaaag
    16621 cttcccgtgt atctccactt agcgctgtac gtgggaatct aatgtccaaa gaaattttaa
    16681 ataacgggaa gacaagtaaa gttgcaaata caggaagttt taaaattgag acttctatgg
    16741 gattaaatca tgctttttca agaaagaaat cttttgtact tatgacttct tcatttgcca
    16801 ttagtattat actattttta ggttttagtg tgcttataga ccttatgtat caaggactta
    16861 agcctatgaa gccagaagct cctcatatat ctattgttga ccctaatttt aagtctgttt
    16921 taactaagga agatattaat gaaataagca atatcaaagg agtaaagaaa gtatacagta
    16981 gaatgatgac ggatatagaa gctacatata atgggaagaa gacaaaatca caattaattt
    17041 cttatgaaaa gtatcaattt aattgggcaa aaaaatatct attaaaagga gaagttaatg
    17101 aaaataaact acagtcagaa aaccttgcat tagtagaaga ggggcatgga tttgatatag
    17161 gagataagat atatattaaa aatagaaatg ctgaaaaaga gttagaggta ggaggtatac
    17221 tttcctcaat tccatttgag gctaaagata aatatattgg aaatattata acatctgaaa
    17281 atacttttac gaatataaca ggtataaagg gttatataat aatggatatt caggttgata
    17341 aagatactcc tggagattta attgaaaata taagacgtgt agtagatgat aacttagagg
    17401 tcagagataa aagacaaagt aacttagagg ttaaaaattc gttttatact atggcaacat
    17461 ttgtatatgg atttgtgttt attattgcga ttataagcat gtttaatatc ataaatagca
    17521 tgaatataag tgttacaagt agaaagaatt actatggtgt tatgcgtgca gtaggcatat
    17581 ccaacagaca attaagaaga atggtaatag tagaggcagg ttcatatgca atgagtggtt
    17641 gtttattagg tagtatacta ggattaatgt tacataggta tatgtttata tcgttagtaa
    17701 gttcaatgtt tcatttagag tggcaagtac cttttaattt actacttatc actgtactaa
    17761 ctatgataat aataacttta ttatcagtaa gaaaaccagt caaaaagatt tgtgaattag
    17821 atataataga ctcaataaat gcacaataaa tatagctgtt gatttaaatt aaaaaaacga
    17881 agtaaaaatg aaatagaaaa tatttttcat ttttacttcg ttttttatat tataaaattt
    17941 ttgttatgtt acaaaatttc tataaagatg atgtgaatct aaacattata tgacaacgtg
    18001 agcatttaag ttaaaatatt ttaaatatac tattttagat aagttccttc ttcatcttga
    18061 taaattaatc cttttttcat aagcactcca agagctcgtt taaaattttt cttacttgaa
    18121 ttaaataata tagaaatatc tttagggtca gatttatcat taaatctcat atatccatca
    18181 gaaccctcta aataacttaa aatcttattt tctaaagtat caagttcatc ctttctattt
    18241 cctctaggag aaagaccaag ttttccatct tcatatattt ttatgacatg taaattttca
    18301 agaacatcac cagcttttag ttcagtaaag tactcattat gaagtattac accagcatat
    18361 ttattgtcta cacatatcat tgcagaatta ttagtttgaa atccatatac aactccagtt
    18421 acaatatctc ctacattgta agtatggtct gttaataaat atgaatctat atctgtagta
    18481 gcagcaattc ttttgctttt gtctaaatat atataaaata aatatttttc gtctctaaat
    18541 agtggataag tcttagcttt aaaaggaact agtatgtctt taggaagtcc catatctata
    18601 aaagtaccaa tgtccgtatt atcaactact tttaaatgtg ctacatcccc aaccttagct
    18661 aaaggcggta ttaaagttgc agcagttctg tcatcagaat ctttaaatat gaaagcattt
    18721 acttcatcac ctatgtttat ttcatttttt ccgattaatc tattatgaag aagtatatcg
    18781 tcttttgtgt tattagtttg tccatccaaa aaatatccaa attcagtttt tctttttact
    18841 gtaagtttat taaaatcacc tatgtttatc aaattaaaat catcctctca ttttatattt
    18901 ataatttagc cataatatac ttatgtagta gtttgtcaca gtatctatat tagtattagt
    18961 gaaatttaca ataaataata tagttattta tgtatttttc aattctctag cttcttatat
    19021 agtataaact ccaatttctt ttttgtcttt aggttttact aaaattttta caaaataagt
    19081 taaaaagtat tgaaataaaa tttcttttaa agtataattg gtatatacca aaattggtat
    19141 ataccatatc gaaaagtggt gactatatga aaatagtaaa taaatctagt aatataccat
    19201 tgcatactca attaagttca ataataagag agatgataga aactggtgag cttaaagagg
    19261 gtgatgcaat aatgccagaa agagaacttt gcaacataca aaatgtaagt cgtatgacag
    19321 taaataaaac cattgttgga ttagtcacag aaggacttct ttatagggta caaggtaaag
    19381 ggacattcgt tgcaaagcaa aaaaagaagt atcagttttc aaatgtaaaa ggttttactg
    19441 atgttatgaa agaaaaggga gtaaacataa aaacggatat attatccttt gagatggaac
    19501 taccagatga tttagttaaa agaaagcttg gaataagtga taatactaca aatatatata
    19561 agattgtcag acttagatat actgatggag agccatttgg attagaaata gtatatctat
    19621 cagaagaaat gtgtaaaggt ctaacaaaag ggatattgga taattcttca ttatataggg
    19681 ttttaaatga aaaatatggc tataagatac aaaaagctga acaagtaatg gagccagtaa
    19741 tactttcaga tgaagaaagt aagcttcttg aaacagatga aggtgcttta gctcttaaac
    19801 ttcatagaaa ttcctataat agagaaggta gccctataga atataccata tcaatattta
    19861 gaacagataa atatcaatat gaaatagtat tgagtgagta ggtgaataat atgaaatgtt
    19921 taattaatgg aaagataata ttaaaaaatc aaatcttgga aaataaagta ttagtatttg
    19981 atgaaaaaat aattgatata gctgatagtg taccaaaaga ttgtgaagtt atagatgctg
    20041 atggaaaata tatatctcca gggcttattg atattcatat acatggaaat atgggaaaag
    20101 atacaatgga ttcgactgat gaatctattg aaacaatttc aaaatcaatt atgagacatg
    20161 gagtaacatc ttttcttcca actacaatga ctatggataa agaacatgtt tatgatgcac
    20221 ttgaagttat taaaaaagcc caaaatagaa agcttgaagg ggcacaagta ttaggagcac
    20281 atttagaagg accttttata aatgagaatt ataagggagc acaaaatgaa aaatttataa
    20341 taaattcaaa atacgaattt ataaaagaat ataaagatgt tataaaagtt ataacttatg
    20401 ctccagaaaa agatattgat tttgatttta ctagagaaat aaaaagatgt acagatatag
    20461 ttttatctat tggtcatagt aatgcaaatt atgaccaagc taaagaagct ataaatttag
    20521 gagtaactaa tataactcac atgttcaatg caatgactgg tttaaaccat agagacccag
    20581 gcgttgttgg tgcagcactt acaactaatg tgtacagtga gctaatagca gatacaatcc
    20641 atataaataa agatttattt caatttatac taaataataa agggaaagaa agactgattt
    20701 taataacaga ttctatagaa gctggtggtc ttgaagatgg caattattct cttggaggtc
    20761 agaaagtcat agttaagggt aatgaagcta gacttgaaaa tggagcatta gctggaagtg
    20821 tgttgtcttt aaataaaatg gtatttaact tcttagataa tacaaacctt aaagtgaatg
    20881 aagcaattaa tttagcttct ttaaatccag caacatcttt gggaataaat gacaaaaaag
    20941 gaagcttaga aataggtaag gatgcagata tagctgtatt tgatgagaat ttagattgta
    21001 aaatgacact ttgcttgggg gacgtagtat ataaaaatat atagtataca aaacaaactt
    21061 taaagacagt aaaaacaata taaaaaataa taaattaaac ttacattagt taaagtagta
    21121 gttaaaattt aggaggaata agatgagagt attagtgtgt aaagattatg atggaatgag
    21181 taaaaaagcc gctgaaatga tagcagctca aatagtttta aaaccaaatt caatactggg
    21241 actagctaca ggaagtacac cagtaggtat gtatagagat ttagtaaaaa aatacaatga
    21301 taatatagta gatttttcag atgtgatgag ctttaattta gatgaatatt ataagttacc
    21361 aatatctaat gaccaaagct atgattattt tatgaaagag aatctattta atcatgtaaa
    21421 cataaagcta gaaaatactc atctaccaaa tggaatggct gatgatattg aaaaagagtg
    21481 tctgaactat gaagcttcta tagatgcagc tggtggtata gatgtacaag tacttggaat
    21541 aggtcgtaat gctcatatag gttttaatga acctgatact aaatttgcaa aaagaactca
    21601 tgtagttgaa cttacagagt ctacaataga agctaatgca agattcttta aatcaagaga
    21661 agatgttcct aaaaaagctg taagtatggg aataggttca atattgaagt ctaagaaaat
    21721 attgctttta gcttcaggag aagaaaaagc tgatgcagta tataatactg tttacggaga
    21781 tataactcca gaagttcctg gttctatact tcaacttcat aaagacacta tagttatagt
    21841 agatgaagca gcagcaagta aactgaatcc aaaagattat aaattagtat aagaatagaa
    21901 tgtagtttaa gtgaaaaata gctatcattg aataatcaaa tttatttaat gatagctatt
    21961 tttatttaaa ttttttactt ctttttattt aaattagtta ttattgttta aattacttgc
    22021 ttattacttt ttacgaaaac tcgttatcaa gaagtatccg aataagatgt taaaaattat
    22081 tatgtttagt gggattctat caagcataat aaaattacct ataattggga tattaccaat
    22141 tggaaacata aagaagaata caggcatcag tgatagtaaa aaagatggaa aacaaagaag
    22201 aagtaatctt ggaatatcaa tataccaact accatccttt ttgtattctc taatcaataa
    22261 ttcagttcct agtaacaaac caataccacc agaggaaatt aaattaataa aaatgtatct
    22321 tttaaagtct tgcatataac cagtgtgaaa ttgagtatag aaatcataat ttaatttaac
    22381 ggctaaaaaa tatataagca atagaatgat taaaaataaa agatatctaa gagctttttt
    22441 cattttaaat tctcccttca gaaatacttt attcagtttt attttgttgg aatctgattt
    22501 tatcatttag tttaagaaat ccataatatt agtgaagaat gaaataagta ttatatatga
    22561 ataaatattt ttagaattaa ggtgaactgt agtgcttaat ataactataa tattaattgt
    22621 ttgaatattc aataaaaagt ttaaaaaata tcaattttga tataaactga ataaatacag
    22681 ttgaatatta aattattatt ttatcaaaaa tatgcaaagt actacacctt caaatatcac
    22741 ctaaagtaat taaaaggcaa ttaaagtgaa gtagttcaag tatttctaaa atgagtttaa
    22801 taaattctat aataatttat tttttgcctt aaaattgcct taaaatccaa acaattttaa
    22861 aactaatgta ctataaaaag ctggtataat ttaaatatag taaaaaataa gatttatctt
    22921 atctattggg atttaattat ttaatcaata tatttatacc atttaattta aataaaaagt
    22981 ctattaagga gtgagcttat gagtaagtta atttatatag cagatgatga agataatata
    23041 agaaatttgg taaagacatt cttaaaaaat gaaggtcatg atgttatgga ttttaaaacg
    23101 ggtgatgaac ttttagaaca atttaatata aaggagtgtg acttagtaat actagatata
    23161 atgatgcctg gctcaagtgg ttttgaagta tgtacaaaac ttcgtgagaa aagtacagtc
    23221 ccaataatca tgcttactgc aagagataca gatatagatt atattacagg gataacctta
    23281 ggtagtgatg attattttac aaaaccattt agcccaatgt ctttggttat gagagtaaaa
    23341 tctatattta gaaggataga gtttgaaaag aaacaaaatt atgataaata tagtaactcg
    23401 atagatatgg aattaaaatt tggtgatgta ataataaata aaaagaataa aatcgttaca
    23461 agtaaaaatg taaatataga ccttacacca aatgaatata atctattgac ttatttgttt
    23521 gaaaatatag atagagcagt ttccagagat gaattattaa acaaaatatg gggatacgat
    23581 atagaagttg agacaagggc tgctgatgat acagtgaaga gattacgtaa aaaaatttta
    23641 gatacaaata ttttaataga aactgtatgg gggtttggat ttaggttgaa ggagaaaagt
    23701 taagatgaat aatggaaatg acaagaatag aattggaaag attaaatcca aatttaaatt
    23761 aaagttctca aagaaaaata taagcataaa aactaggatt ttgttgagca tactttgttt
    23821 gataattata gtatttatga taatattagt atcttttaat gtatttcttg atacatatat
    23881 aaaaacaact gcgaatgaag aattgacgaa gtctacagaa atagtagaac atatggatag
    23941 taattttaga cctattaagc caccacaaga tgatggaaag cttcctccca aagtattatc
    24001 taattttatg agaaatgtac aagataaagt taggatggca gaaacacaat cagatgctga
    24061 tgctatggta gttgattcta agtataacct gatatttcca acaagagagg atgatttctt
    24121 aaaaaatgtt gatgagatgg agctgattag ggagtgtatt caaaatgaaa aattagattt
    24181 aaattcagat gaaaatacac ggatatctac cagaaataag gattattata tttcaactgt
    24241 aaaaattact agtatatatg gtgaacaaga caaatatctt atacttttta tagacatatc
    24301 aaaaactcta aatctagcac aaaagataaa tattgtatta atatctgtaa tgtgttttgc
    24361 tggaatatta gcaatattta cagctgtgat tttatctgag aaaattgcta aaccgataaa
    24421 agagttgtgt gagtttgcaa aaaaaatggg tcaaggagat ttcaaaagaa cctactttga
    24481 cttttcagat aaagaattag tagagctctc aactgttatg aataagagtg ctgagtatct
    24541 ggataagtat gataatgaac aaaaaatatt ttttcaaaat gcatctcatg aattaaggac
    24601 accactcatg tctattaaag gttatgcaga agctataaaa tataatgtta tagatagtaa
    24661 acatgcaagt gatataattc ttgaagaaag tgataggtta agtgatatgg ttgaagattt
    24721 attatacatt tctaaaatag ataatataac taaagatttt gaacttgtag aatgtgattt
    24781 aagagaagta ttttcaaatt gtggagctag gcaaaatgta agagctataa ataaggggat
    24841 aaaatttatt tatgaatttg atgaagaaat ggttttattt gaatgtgatg aaaaaaacat
    24901 ttccaaagca tttatgaatc ttatagaaaa tgctttgaga tacgctaaga gtgaaataaa
    24961 aatagtgtgt aagtataatc aaaaaaatat agttgtgata atagaagatg atggaattgg
    25021 aatcaagaaa gaagatttac ctcatgtttt tgaaagattc tataagggag tagggggaaa
    25081 tcatggaatt ggattatcaa tagttaaatc tattgtaaat aagcatggtg ggcgtatata
    25141 cgtagaaaat ggcaaaaaag gagctaagtt tactatatta tttaaattgt aaaaaaatta
    25201 gagaaaatat aaagatgttt gaagttgttt tttatatata tctgttgtat actgttgaaa
    25261 aaagaacaat attaagctga catatattac aataattgac atttaaagta aatgcttaaa
    25321 tatagatacg aaaggaaaag taaaatctga agtgtattta ggtttaagta agcttcttat
    25381 tttataaaaa atatgtatga aagaacagct gatttttaat attacaaatt tagtaacggt
    25441 tgtatttgaa gcttttgtca tccatatgtt tttatcagac tttttaggtc agaaagaagg
    25501 gtatataaaa gttgtaagat atgctaaact gggttttata atatgtttag gattctgtaa
    25561 tttaataact ttaaacccta aaatcacaat gcctttaatt tttatcctta ttttttcaac
    25621 atcattttta tataaaggaa atttaaagac acgattgttt actactgttt tattaagtat
    25681 attctttata ctgtcagaaa ttgtagttac aagtattttt gttttatttg taaaagaagg
    25741 ctttgaaata atgcttgaaa ataatagtat aagagtattg gcgacaattt tatctaaaat
    25801 tgtctttttg ttgacttgta aaattatttg tctatttaaa aaagatgtac atttagatat
    25861 gcccataaag tattggctcc ctttattttt aatacctata tttagtttat ttctgtctgt
    25921 ttctatcttt gatgtaagta aattttttag tcttgaaagt ttaaaatttc ta
//
LOCUS       DAMKPG010000054        25922 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMKPG010000054 DAMKPG010000000
VERSION     DAMKPG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.057"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(224..1072)
                     /locus_tag="PC621_002267"
     CDS             complement(224..1072)
                     /locus_tag="PC621_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF4109417.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            complement(1108..1578)
                     /gene="trmL"
                     /locus_tag="PC621_002268"
     CDS             complement(1108..1578)
                     /gene="trmL"
                     /locus_tag="PC621_002268"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF4109418.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            complement(1772..2824)
                     /gene="nth"
                     /locus_tag="PC621_002269"
     CDS             complement(1772..2824)
                     /gene="nth"
                     /locus_tag="PC621_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF4109419.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            complement(3021..5258)
                     /locus_tag="PC621_002270"
     CDS             complement(3021..5258)
                     /locus_tag="PC621_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4109420.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            complement(5364..6278)
                     /gene="queG"
                     /locus_tag="PC621_002271"
     CDS             complement(5364..6278)
                     /gene="queG"
                     /locus_tag="PC621_002271"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF4109421.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7278..7682
                     /locus_tag="PC621_002272"
     CDS             7278..7682
                     /locus_tag="PC621_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF4109422.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            complement(7939..8934)
                     /locus_tag="PC621_002273"
     CDS             complement(7939..8934)
                     /locus_tag="PC621_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF4109423.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(9186..10022)
                     /locus_tag="PC621_002274"
     CDS             complement(9186..10022)
                     /locus_tag="PC621_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF4109424.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            complement(10510..11073)
                     /locus_tag="PC621_002275"
     CDS             complement(10510..11073)
                     /locus_tag="PC621_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109425.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            complement(11171..12100)
                     /gene="glsA"
                     /locus_tag="PC621_002276"
     CDS             complement(11171..12100)
                     /gene="glsA"
                     /locus_tag="PC621_002276"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF4109426.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            complement(12665..14332)
                     /locus_tag="PC621_002277"
     CDS             complement(12665..14332)
                     /locus_tag="PC621_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF4109427.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(14406..15161)
                     /locus_tag="PC621_002278"
     CDS             complement(14406..15161)
                     /locus_tag="PC621_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF4109428.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            complement(15171..15707)
                     /gene="lepB"
                     /locus_tag="PC621_002279"
     CDS             complement(15171..15707)
                     /gene="lepB"
                     /locus_tag="PC621_002279"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF4109429.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(15873..16409)
                     /gene="lepB"
                     /locus_tag="PC621_002280"
     CDS             complement(15873..16409)
                     /gene="lepB"
                     /locus_tag="PC621_002280"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF4109430.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(16509..17819)
                     /locus_tag="PC621_002281"
     CDS             complement(16509..17819)
                     /locus_tag="PC621_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF4109431.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(17898..18614)
                     /locus_tag="PC621_002282"
     CDS             complement(17898..18614)
                     /locus_tag="PC621_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF4109432.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            18859..20130
                     /gene="sleC"
                     /locus_tag="PC621_002283"
     CDS             18859..20130
                     /gene="sleC"
                     /locus_tag="PC621_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF4109433.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(20600..21256)
                     /locus_tag="PC621_002284"
     CDS             complement(20600..21256)
                     /locus_tag="PC621_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109434.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(22369..22779)
                     /locus_tag="PC621_002285"
     CDS             complement(22369..22779)
                     /locus_tag="PC621_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109435.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(22995..24377)
                     /locus_tag="PC621_002286"
     CDS             complement(22995..24377)
                     /locus_tag="PC621_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF4109436.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKSGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(24388..24747)
                     /locus_tag="PC621_002287"
     CDS             complement(24388..24747)
                     /locus_tag="PC621_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4109437.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(24952..25731)
                     /locus_tag="PC621_002288"
     CDS             complement(24952..25731)
                     /locus_tag="PC621_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF4109438.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         8440 a         4743 c         2473 g        10266 t
ORIGIN      
        1 aattttttta tatacaattt acaagtacat tttgaaattt ttttaataaa tcttactata
       61 aaaaagtgtg caatagattc ttatctattt acacacttcc ctacaaacta aatctaatat
      121 atcaacactt aatttcatcc aaaataaatc caatttaaca tctcaataat taatattcta
      181 tcctaattta ataaaaaaat aaataatgtc atccaaaaat atattattta ttttttaagc
      241 atatgatacc agtgacacca ggaccagcat gcgcacctat acacacacca atttggaaaa
      301 atctaatttc tttaggtctt aattcatctt gcataacctt agataattgt tctttttcct
      361 taaaatcatc actatatcct atatacacaa tttggtctga taaatcatcc ccacaatttg
      421 attttatcaa ttccatcatc ttagaaatta cattcttctt tcctcttact tgagatattt
      481 gcgaaactag tccatctctt atttcaagaa tcggttttat atttaatata cttcctattg
      541 ctgccttagt agatgatatt ctacctccct tttgtaaata ttccaatgta tctactgaaa
      601 atataacaaa agccttctct tttaattcat ccaaaacagg taatatttca tcaacagact
      661 ttccttcact tgcaagttct cctgctttta ttacaagtaa accagttccc atacaaagca
      721 agttagagtc atatgtatat attttaccat ccacatcatt ctttgccatt acagcacttt
      781 gatacgtccc tgtagctgtg gcagaccctg ctatataaag tatctctcta tcctcatttg
      841 tatatttctc aaaaatttcc ttaaattggg cataagtagc ttgagaagtt tttggatata
      901 cattttcttc tctaagcctt ttataaaatt catctccaga tatatcaatt ctatccttgt
      961 attctctttc ctccagtata actgttaatg aaactatatc tatatcatat ttatctaaca
     1021 aatcttttgg cacgtccgat aaactgtcac agattatctt tagatttttc attaagtatg
     1081 ctcctttctc atttaggctt gttgaaacta cctcatattt gggtatccta tttgtcttaa
     1141 agcctcgtac actacaatag caactgaatt tgataaattt aatgacctag ccttttcaac
     1201 atccaacata ggcactctaa tacaagtatc catatttgct ttcaacaaag gttctggtaa
     1261 tccctttgtt tctttcccaa atactataaa acaattttct tcatatttca catctgaatg
     1321 tgactgttta gcctttgttg tagaaaaaaa gtatttacta tttggatact tttcttgtaa
     1381 ttcttcaaag ctatcgtaat attgtatatc agctatatcc caatagtcaa gcccacatct
     1441 ttttaggtgt ttatcatcca atgaaaaacc cagtggtttt actaaatgta aagttgaacc
     1501 tgttgctgca caggttctta ttatatttcc tgtattttga ggtatttctg gttctactaa
     1561 tactatattt aatgacattt taatcctccc taagtatcgc ttttatttat caaattttat
     1621 tttataaaca gaataacaat aatattatac tattatataa aataatgtta ataataattt
     1681 ttatttcatg tatataatag ttttttatct aacattttca caagtattat tatatcagaa
     1741 aatccctctt gtacaactta ctttctaaaa gttatttcgt ttcattaagt tctttataat
     1801 aattacaatc ttcttttatt gggcaacttg cacattctgg atttctagct ttacacattc
     1861 ttcttccatg aaaaattaat acatgatgtg agtgtgacca tctttctttt ggtatagctt
     1921 ccataagtgc aaattctgtt ttttgaggat ttggctcgtc cactattcct attctattgc
     1981 taactctaaa tacatgagta tctacagcta ttgctggatg attaaaagcg ttactaagta
     2041 caacccctgc cgtttttctt ccaacaccag gtaattttat aagcttttct aagctatcag
     2101 gtacttcacc atcatataat tcacatagct gttcagatgt atcctttatt ttttgagact
     2161 tgcttttgta aagaccacaa ctctttatct ctttactaat ttcttcaatg ctcaaattag
     2221 caaagtctct agctgtgttg tactttttaa acaactctga agtaacttta ttcactctta
     2281 catcagtaca ttgtgctgat aaaatagttg ctattaaaag ttcaaaggct gtcccataat
     2341 ttaactcaca tttagcatct gggtacaatt tttccaactc atctaaaatc ttatttacat
     2401 cttttttatt ggctgattca cttttaattt tagacttact tttaacttct ttgctttgtt
     2461 tttgtgtagc cttattagaa ctacttttct ttgttttttt ctccgtttca ccttttttag
     2521 tacttttttt acttactttg atactttttt tatcttcttc aatataccta aaattctcta
     2581 tatctttctc atcaattatc tcatcttttt tactaatcat tttatctatt tctttttgaa
     2641 cctcaacctt ctttacattt tttttatcta tctcttcttt ctcaattata tctttcttat
     2701 ctatactttt tttatttact tcttccttct caattacatc tttcttatcc attcctttta
     2761 gatttatatc ttcttccaca attacatctt tttcatttat ctcttttaga tttacatttt
     2821 tcataattat ccccctttaa aataccaatt tataaaacac tatttataaa tagtttagtc
     2881 atatcaacaa aaaataaaca tcaatatata aaacatacaa aataagaatt taatttatat
     2941 atatttacaa aaaaagcaaa aacctattaa agttcttgct ttttattata cataaatacc
     3001 cattaaattc tataataaac ttattctttt gacacagttt gtatcttgct gaaagatatt
     3061 aatttttctt ttaccaattt ctttatttct tcaaatttaa catctgtcaa tatctctata
     3121 gcctcttcta ctctttcaac agtatatatc ttaaattttc catctcgcac tgcatcatta
     3181 acttcatctg ataaaacgag atttctttga ttttttctag gaattatgac gccttgctca
     3241 ccatttaacc ctttagtttt acatatagaa taaaatcctt ctatcttttc acttatacca
     3301 ccaactactt gtatatcacc cttttgattt atagaacctg taacagcaat attttgttta
     3361 attgatactc cactaagaga tgataatagt acatataatt ctgcagaaga tgcactatct
     3421 ccatctattc caccataatt ttgttcaaaa caaacatatg cgtttagtga caatagtaaa
     3481 tcctgtgcaa aattctcaga aagatagcca cctaatataa gcactccttt gttatgtata
     3541 gagccactca tatttacttc tctttctata tttactatgc ctttacttcc tgggctagtg
     3601 gtcgctgtaa tccttgaagc tctaccaaaa gagtattctc ctgtacttaa gactgacaga
     3661 ccatttatca ctcctacttt tcttccctct gtttctatta gagtaaatcc attttcaatt
     3721 gattcatcca tatgtgtttc cactctattg attctttttc tcttttcact tattgctttt
     3781 ttaacatcac actgctctgt atactccgaa tttctaaact ctgcataagc acttccttct
     3841 attacaactt ccattatttt attaaactta gttgatagtt tttctaaatc accagataat
     3901 ctagtgctaa atttaataac ctcttcaact gctgatttag taaaatgcct taaattattc
     3961 ttatcacatt ggtatgctat aaatcttgct attccttctt cattatcttc atttttatcc
     4021 atttcactat caaagtctac aaatacttta aaatattttt caaaatcttc atcatatcta
     4081 tataaaatat catatatata gtggcttcca atcagtacta atttaacatc caatggcata
     4141 ttttctggtt taattgctgt ttgtgtttcc aatattactt ttttagtctg caaagtcttt
     4201 ttaagaattt cccaagacat tgtatatttt agtaattggt ctgcataaag tactaaataa
     4261 ccaccattag ccctatgaaa tgctcctgat aatagttttg tgaaatcagt ctttatattt
     4321 ccatttgcat aatcatactc tgcctttcca aatagatttg ctggacttgg gtttatctct
     4381 actacaacag gtgctgattc tttattcttt gagctttcat tgtctacaaa taggttaatt
     4441 ttgtatttaa taaaatgctc tttatcatac ttatctttta actcctcttc atctaaatag
     4501 aacaaataca tatattctat tatatctttc tttatatttt ccagataagt ttgaatctta
     4561 tcataatttt catatttttc acacaatctc ttgatatgag gttctactac aagtttagct
     4621 atctcttctt caagctctaa cactgctttt tcagctattt cttctaagtt tctaatttta
     4681 tatacgacct gtatcgccat attttctagt tctcttttag ctttgtaaaa ctcttcatca
     4741 gacttatcac tttcatcttc ctcttctttc aaagggataa aaactattcc cattttactc
     4801 tgttttaatt taaatccttt ttcttcccca tatttcttaa tttgcttcaa aagcatatct
     4861 ttttctattt catattcatc tagaagtcta tttttttcta tttcaaaatt ttcactttca
     4921 aacgcatttt taaaatcttc taaaagactt tcaattaatt tctccatatc tttcttaagt
     4981 tctcttccaa accccttttc taattcaact ataataggct ctctagggtt ttcaaaatta
     5041 tatatataac accaatcttt atgtgtattt tttttggatg catattcatt taaaaccttt
     5101 aaggcataag tacttttacc agttccaggt tcaccagcta tatatatgtt gtacgctgga
     5161 ttatttattt tcaaccctaa ttccatagct tctacagctc tttcttgacc caatatataa
     5221 tctagtggat ttatttcaga tgtattatta tatttcaata ttcccccacc tccccatatt
     5281 taactttaat gatataatat gacttttaca caacaagtgt taaagtaaat acacattaaa
     5341 gtatatgaaa cagactctta ttattagtca tcatacttat ctaaaattat ttccatattt
     5401 ctctttatta tatttctccc tctccagcta aaagctctat ctccatatct ccttttaaat
     5461 tctttatttg aaatattttg tatctcatca taatccaaat aatctattat attttcttta
     5521 aattccaaaa tatgagttgt tggcatttca acattatgtg gacatacttc ttgacaaata
     5581 tcgcatccaa ataccttttt gccattcttt aaaacaactt tttctttctc tgacaaatct
     5641 cctttttttt gagttatata tgataaacat tttttggggt tcatatcata atttcccaat
     5701 atagcatttc ccggacaata ttttacacac ttaccacatt taaaacaact tttttctagt
     5761 ggtttatctt cttcaaattt ataattattt actatatacc ctataaatac gtaagaacca
     5821 tattcatcag ttatgatatt attatttatt ccaaaatatc ctattcctga taaataagct
     5881 aaatacctgt caactaatgg tccattatca acaaataact tgtatttaaa atctgttact
     5941 gttttagaaa tataatcaca aatttgttgt aaaaagtcct ttactacaat gtgataatcc
     6001 tttccatgac aatattttga taaatttgaa tttttattct tttcttgtct atttatatga
     6061 tatggaaatg cacagactat tatagagtta gcatccttca ttatatgtct tgggttaatt
     6121 ctgttctcaa ttattggttc ttccatacca gttaaataac ctttttgttg tctgtcctta
     6181 agtatctttt ctaaattatc atacttatct acccctgcta ctcctacaca gtttaatcct
     6241 attaaattac aaaatgcttt taattcctta ttatccataa aattattcct ttttaatcta
     6301 attgactttt taaatttatt taattttttt agtcttttta accttttcta gctactaact
     6361 cttttaaata cttcgtcttt tttatttatt taactttttt acctactaac tcttttagat
     6421 acttcgtctt tttacctatt tagtttttta cctactaact cttttagata cttcatcttt
     6481 tttatttact tatttaactt ttttatttac taactctttt agatacttcg tctttttcat
     6541 ttacttattt aactttttta tctgcttaac tcctttaaat acttagtctt tttatctatt
     6601 taattatttt acctactaac tcttttgaat acttagtctt ttttatttat ttaatttttt
     6661 tatctactaa cgcttttaga tacttaatct ttttatctat ttaatttttt tatctactaa
     6721 cgcttttaga tacttaatct ttttatctat ttaatttttt tatctactaa ctcatttaga
     6781 tacttagctt ttttcattta cttatttaac ttttttacct acttaactct tttagatact
     6841 tagccttttt aatctattca acctttttta cccacttaac cttcttacct tttaatataa
     6901 actctaaaac ttcaaatatc attagttata ttttgacatt cttcctatag ttaatgcaaa
     6961 aagattgatt ttttcttata attaatatcc catttacatt gccatttatt aagtctttac
     7021 ttggcttata tggatttctt tttactctct ttactaaacc tatacttatt aatattatga
     7081 aggtcttata atagtctaat atgaatatgt ccatcatata tcaaatttta aaatttgtaa
     7141 gcaaaatact tatcttatat tactacatct aaagtggaaa attttacaaa caagccctgt
     7201 aaaaacactt taaataacaa tttgctttta aatcatatac agattataat aaatactgta
     7261 taaggatggt gaaaaatttg attaaaataa caattccagg tagacctatt agtaaatcta
     7321 attttaagct tcataacata aatggtcaag catggatgcc ttcaaaggga aaatattcta
     7381 agtatcttgc atacgaaaac atgattgcag gttttataaa ccaacagtat caaggggaaa
     7441 ctgttgaaga aaatctaata acagtcttga aattattctt tccaaataag agaatggggg
     7501 atttacataa ttatccaaaa agtatttgtg atggtattga aaaaagtgga ataataaaaa
     7561 atgataaaca acttaagcca gtatttttat ttgattttat agataaagat aaccctaggg
     7621 ttgaaattga actttatcca gtatctaaat acgatatatc ctataatatt tttgaaaaat
     7681 aaagatttta tgtgttattc gacattatat tgatactcca gtatttgaaa ctatactaat
     7741 acaacaataa taattgtgtt atgtctatac tcaaacaaat gtttttaaaa agcattaagc
     7801 aaatatttaa taagtattaa acaaatatta aatttatata atagctataa ctatgtaaat
     7861 ttaatatttg tatataagac cacatctaat aaaaagaaat cggagtaaat ttaatacacc
     7921 gatttcaata aataaaatct atcctagctt ttcaacaaca gtcttaagca tttctcttat
     7981 aggaagttca tatggacatc tactctcgca aagcccacag tcaatacact cagtaggttt
     8041 aacatccata acctcatatt tttctttagc ccactctttt aatccatatc tagtataata
     8101 accttcacat aaaaaactca atggtatatt tattcctaca gcacaaggca tacaatattc
     8161 acatcttcta cagaattttt tacctaaaga atttcttatt tcttcaattt ccttattatc
     8221 tttttcatct agaacaagat tttcaagtac agcgacattt tgtctaacct gttcaacact
     8281 ctccatacct ggtataacta catctatgta atctttagat agtatatatt taatagccag
     8341 tgttgcattg tctaaagcac ctccagctaa aggtttcatt actattatcc caattccttt
     8401 ttcatgagct tttttaaaca cttcatctgc ttgtccttca actatattgt aagggaattg
     8461 tatagtgtca aattttccat cttctatagc tttttctata gtatttaaat tgtgactagt
     8521 tataccaata tgttttatct taccttgctc ctttgcctct aaaagggcac tataagccat
     8581 tttatcttta aaaagattat catactcctc ttctttaaca ttatggaatt gatataaatc
     8641 tataaaatca gttttaaaat tatttaaact tatttcaaca tctctcttca ttgaatcata
     8701 atctctggac atacttttag tagctaagaa aaatttattt ctttttcctt caatggcaat
     8761 tccgattgct tcctcactta ttgtataccc tcttgcactg tcaataaagt ttattccttg
     8821 tttttctaac tcatttataa ctaagttagt atcatcttga gtaattcttt gtattggaat
     8881 gcccccaaag ccaactctct ttattttcat gttagtattg cctaaatttc tcatatcttt
     8941 ccccacctaa cttttaataa ttttttatat aatataaata tgttaccttt aattatacaa
     9001 caaattatga gaatctttgc aatatattta catactattc ataatataat catactttat
     9061 acccagttaa tttgtataca atttttgtta ttcctatcca ttaatttaaa ttatcacatt
     9121 tcatgattgt atctccacct taattatgtt gagggcggag tatttcacat atttttaata
     9181 aaggtttatt tactagatag tatttctttt atcattttaa gctctctctt gtgaccttca
     9241 tcatcaaaag gagtttctaa gaaaaatggt aaatgtttta gactaggatg ttccataaaa
     9301 tcaattaatg cttttaatcc tatctttcct tcccctatag gagcatgtct gtcttttttg
     9361 tctccaaatg gcatcatact atcatttaga tggacagttt tcaatctttc tatgcctata
     9421 accttatcaa attcttccaa aaccccatct aaatcattca ctatatcata accggctgaa
     9481 aaaatatgac atgtatcaag acacacacct attttttctt tatgctcaat tttgtctatt
     9541 atacttttta attgctcaaa tttaaaacct atttcagttc cctttcccga catagtttca
     9601 agaagtatag ttatattttc atcacccttt atggcattgt tcaatccttt tactattcta
     9661 tctataccaa aatctacacc accacctata tgacttccag gatgaaaaca catgtattca
     9721 attcctaatg agtccatcct tcttatatcc tcatctatca cccttgttgc aaattcgtat
     9781 acatcatcct ttgtaccacc cagattcata gtatatggtg catgtgcaag cagtggtgca
     9841 aaattatttt cttttctaat ttcttgaaat ttaattatat ctttttcatc aaattcttta
     9901 gcatttcctc ctcttggatt tctactaaaa aattgaaatg tatttgcacc aatatataca
     9961 gctgtctcag ccgcattagc aaatcctttt gctattgaaa gatgtgttcc taatttaatc
    10021 atattaatct ccttttattt gtactacgac ttgtactcaa ttttaatttt gaactactcc
    10081 accttaacta tatttagcca acatatgaag atggagattc ctatttgata gtactttttc
    10141 actaaaaatt cataagctct aagggatgtt ctatcccaac taatctcatt cctcttttaa
    10201 catatttcta gcattgattt aatttatatt aatttttaat tttaatattt acactttaat
    10261 tatacccatt ttaatcaatt tcaaatatta taatcttaca tactttgtat attatctaag
    10321 cacttctcag atagtgtata catcttacac tatacatatt atttacaaat acaataggta
    10381 taagaaaaca aaaaaataga gattaaaaat cattgtttta cccataccaa aaactatttt
    10441 ttgattatta agtaaatttt gatgttcaat ctctaatatt atatctttaa ttaatccata
    10501 attaattctc taactaggac attcctcctc aagagtagga actataacca tagctatatc
    10561 atacttagaa ccttcaacat tgtgttgtct tttatttaaa atttcatcta tctttgactt
    10621 catagtgtca ctaagttcaa ctaattcatc atgagtaaga gaaatatcat gataatcaac
    10681 tatattttca ttttctaaaa catcttcctc tgctgcctta taaaaaactt cactcatatt
    10741 ttcaaatttt gatatgtaca attcttcttt ttcttcccca ttgtctaaag aaaaattaag
    10801 tatcctattc cctataacca cattttttgc acttggataa tagtactttt ccacaatccc
    10861 cgactttatt ttttcctcaa ctaattctaa aataccaact ttgtataaca tttttatatg
    10921 gtagtttatt tttgcatgag gttcttccaa taattgagat aattgttttg ctgacaatgg
    10981 cgatttgtca aatgctttta gaatatctat tctacgtgga tgcgcaatag cctttatgca
    11041 ctccaaatct cgtaatacca atacctcttt cattgacccc tcctaaaaaa tttatttaat
    11101 tcatatttct ttacaattta attatatatt aaattatatg ttgttgtcaa aaaaatcctt
    11161 tcttaatatt ttaaaaaata tttaatttga gtttatttga caactcctct aaaactttaa
    11221 ctcctgcgac gctgttacct tttttgtcaa gcgcaggtcc ataaacacca atccccatgc
    11281 gtcccggcac agaagccatt ataccacccc caactccaga ttttgcagga atgccaactt
    11341 taatagcaaa ttccccagaa gcatcataca ttccacaagt catcataaat gtcttgacaa
    11401 tcctagaaac catttcactc attaaatgct caccattttc tatatctact ccataatttg
    11461 caagatttat tccaattctc gctaaatcta cagaatctat ctcaattgag cattgtttaa
    11521 aataaaggtc tagaacatct tctacattcc ctctgataaa tccatcactt ttcaataagt
    11581 aagccatagc tctgtttcta tctccagtca ttttttcaga cttatagaca tcatagttta
    11641 ttcctatatt atcatttttt gctaactttc taaagaaact aagcattctt tcttcttttt
    11701 cttctaaaca actcccattt ataagggaag tagttactat agcccctgca tttatcattg
    11761 gattacaggg tttttttgtg tcattaattt caagtttcat tattgaatta aatgggtccc
    11821 cacttggctc cattccaacg tttgaaaaaa cgtcttccca atcattatcc ataagtgcca
    11881 tagcaagcac aattggtttt gaaatacttt gtatagtaaa ctttgtgcta cagtttccag
    11941 cactataaag attattttcg ctatctatga tacaaattcc taaatcattt cttctagcgt
    12001 tttttaattc tggtatgtaa ctagcgactt gcccataatt agtatacttc ttattagaac
    12061 ttataatttc ttttaataat gtctcatcta acgtattcat atgcacctct tatattatgt
    12121 tatatattct attttagcac aagtttgtac tataataaaa cagtgttaaa aaaaattata
    12181 ctttaaaact attttctaca agaatcaatt catttttcaa acaatttaaa cagtttaaat
    12241 agcttaataa tttaataatc tagtaattta gtaatctaat aatccagtaa tttaaattct
    12301 tataacgtca aaaatttcta acctaacatc ttttactctt ttactctttt actcttttac
    12361 tcttttactc ttttactctt ttactctttt actcttttac tcttttactc ttttactctt
    12421 ttactctttt actcttttaa aaaattatca cataaactaa caaaatatta aacaaaaaag
    12481 taaaatttaa aataaaaagc ttgcttcata caaaagtata aaacaagcct ttttatatat
    12541 caactcaaac tttaaaatct gtatcaattt aattctccac tttacactta atatacttaa
    12601 cataaaccaa tcacactact tgctatgcca tttaatcttc ataaaacaat ctataataca
    12661 catattaatc aactatttta cttcctccaa taaattccct aagaattgaa atacctggta
    12721 tgtcagaagt atcgtccaat tcaacaaaat actgcaaaaa ctttagtaat ctagtagctt
    12781 caatatgtgc aggattatct tcctttatct cttccaacaa aggtttcgca aatcgtttta
    12841 aaactgctaa ttcatataca caagcagagt taaatatagc atctgcctcc tcttggtacg
    12901 ggaatatatt cttcttttct cctcttctaa ctgatgccca ttgtaaaata gtattatcag
    12961 cactatatcc tctaaaatta tagtctctaa caattcttct tatcattctc aaatcagcag
    13021 tagggattcg attatggtca tccaaattta tttgagtaag ggcacttatg tatatcttaa
    13081 atttatcttc atctggtata gatgatgtta aaataggatt tagcccatgt ataccttcta
    13141 atataattgg ctgatttgaa tctatcttta tttttttacc agtgtattct ctcttacctt
    13201 ctttaaaatt gaatcttata tcatcaattt cttcaccagc taaaagtttc tttaagtcta
    13261 agttaaactt ctctaaatct attgcataaa ttgattcaaa atcataattt ccatattcat
    13321 ctaaaggagt atgttttcta tctataaaat aatcatctaa agaaattgaa ataggattta
    13381 aattatttac tctaagatgt attgataatc tgtgagcaaa acttgttttt ccagatgaag
    13441 atggagctgc tattaaaatt actctcttat tgccatcctt tatcatatcc gctatttggg
    13501 ataacttctt ttcatgcaat gcttcgaatg tccttattat atctccatat tcattgtttt
    13561 ctattatttt gttaagagat actaccttgt caattcccat aagtcttgac cattcttctg
    13621 cttcataata tacacttgat agcttaggtt gaggtacaaa cttcatagga agagtcttat
    13681 cttcttcact aggccctaaa attataacgc ctccattata aagcttaata tcaaatgatt
    13741 ttatataacc agttgatggc aacatataac cataaaaatg gtctatatag tttctacact
    13801 tatatacttt agcactatca tactctttgt atttcaaaag ctctgccttt tcatacattt
    13861 cattattttc aaatatagaa atagcttcat ttcttgtaac ttcaattttt tctataacat
    13921 aatcgttgtt tactatctct ttcatcttgt tttttatatt ttcaacatct gcttctttaa
    13981 gctttctatc tatatgaact tcacaatata gcccatctga tagtgaatgt tctattgtaa
    14041 ctctgctatc ccaaaagatt tctctacaag ccattacaaa tataaatgac atgactctaa
    14101 agtaaactct ctctccatct tcattagtag tgtctaaaaa atttatctca caatccttct
    14161 taactctaca atttagctct tttagtttat tgtctacaac tgcaagtgtt atataaccct
    14221 tatgctcatc acaaaattct ttcgctatat ctcccaatct gattccaggg gaattttctt
    14281 ctaatgaata ttccttatct tttccttcta tttttaagtt tatttttttc acttcaacat
    14341 ctccttcctt ttgatgaata taaattttag ttaatgaggc atatcaacaa gataaagaga
    14401 ggtatttaac cctctcttac aatattattc aatttactta tagtatcttc aatgtcttcg
    14461 taatttattt ctaatactaa atattctgca tctaatgtta agacctcttt aatcccctct
    14521 tcagataaaa ttcctcttcc tataccaaca tgtgtatcat tgataccatc attatcactt
    14581 aagtgtatca gtcttatttt atcctttaag ttctttacat aagcatcctc atcaattaaa
    14641 cagtgtccag tatcgtaaca aaaacaaatc ttagaatttt taatcctctt aaatatatat
    14701 tcaaaatcat aagatatatt tccaatattt gaaaaatccc caccttttga ataagtattc
    14761 tctatacata catatgaatt tgtatttaat ttatctaaaa aatccacact atttcctaaa
    14821 tatttgtctc tattctttct aactctattt gtcataacat atcccaaatg aaggttaaaa
    14881 taccttatat caaatccttt tagttcaaat tctatcttct caataaaatt tatccatgaa
    14941 tttcgtatat actctatgtt ttcgcaagta tttaattcca taggcaagtg tatccctata
    15001 gataatccta acttagaaat tctctcttta tatttacata actcgctaca ttcagatata
    15061 ttgtcaattc caatctctaa atgagttatt tgctttatac tctcacaaat gtttaaagcc
    15121 tcctcaatat taaacaataa ggacgctata ccaactgcca tacaaatcac ctactcaaaa
    15181 atacctatat ctgtaaaagg aaatactctt aaaataactt ttcctttaat attttcttca
    15241 tctaccaatc ccacttcttt atatctactg tctaaactaa cttctctatt gtctcccatc
    15301 gcaaatactt ttcccttagg aatcaccata tcaatatcgc cttcagtata attatcatgt
    15361 atatatgaaa cttcatttaa taacttacca tttatataaa ccttagaatc cttaattttt
    15421 agatggtcac caggaacacc tataaccctt tttacaagct cttttttcct tccattctct
    15481 tgtaataaat cagtcttaaa tatcattata tctccacatt ttggttctga taacttatat
    15541 gtcatcctat ttgctaccaa ataatcatgt gatttaagtg tcgggtacat tgattctcca
    15601 tttactagtg ttggttttat aaaacgagtt ataataaatg caaaaaaaag tgcagtaata
    15661 attattttta tccactctac aatctcttct ttaatagttt cattcattca caaattctcc
    15721 tttatagtta tattatcttt attgataatg tctcttaaac actatctttt agataagtct
    15781 actaaataag gtgttgtctt ttttatatag acaactattg tgtatggtct ggtgatttca
    15841 gctaatcagc gattgctcca aattttatat gtttagtcaa tagttcctat cttagaaaat
    15901 ggatacaatc tgactagaac ctttcctaaa atggtatgct cgtcgaccaa tccaacctca
    15961 tcgtatctac tatctaaact tttttctcta ttatctccca ttgcaaataa ttttccttct
    16021 ggaactacga tatcaatatc tccatcagta cgattattat gtatatagga agtctcatct
    16081 aacaacttat catttacata taccttggag tcttgtattt ttacatggtc accaggaacc
    16141 ccgataactc tttttactaa atcttttttc tttccatttt cctctgttaa gtcggtttta
    16201 aatactatta tatctttgta ttttggctct cctaccttgt acgcaattct gttaactatc
    16261 aaataatcac gttcaactaa tgtaggatac attgattctc ctttgactat tgttggcact
    16321 ataaaacgag ttattgcaaa tgccaaaaca agagctatga caatcacttt tatccattct
    16381 acaacttctt ttttaactgc ttcacccact aaaatcgtct cctctgtgtt aaatattaag
    16441 tcaatctaca gcaaaataat ctaccataaa cctacaatat aaatattata cttctttttt
    16501 ctaatagttt aaaatacttt tctaagacct ttaggataat gattcttaat tacaccagaa
    16561 gattcttttc cccaacctaa aggtattcct tcaaccgaaa ccaaaaccca tcctctacta
    16621 tcattagaat tcacaacatc cccttttaaa tatttaagaa ttgattcact ctgtacactg
    16681 aagttttcaa cattttttac atcttcaact tttaagtaat gagatagtgc atgagaaggc
    16741 tcaaatctat tcttctttaa aacccctaaa tgcaaaccgt atctcaatac ttttagtttt
    16801 ttcgtatctg gatatatttc aggaagtagg tacaaatttt ctcctcttaa ataaaattta
    16861 ttttttttat tactataaaa atcaaccttt aaaaatttct tttcaaactc cctataatcc
    16921 tttatttctt tatctaaatt ttttattttt aattctttga cattacattc ttcttcttct
    16981 aattttttta ttttggcaac aaaatgacct tctcccttta tcttatgagg ccataatctt
    17041 tccatttcaa taagttctgc atgtggatat tcactgataa actcatttat aacatgctca
    17101 ttttcctccc ttgaaaaagt acaagttgag taaattaaaa tgccatcctt ctttaacatt
    17161 ttatatccat ctctgataat ctctttttgg atggattgac attctactac ctttgcataa
    17221 ctccaatctg atatggcaac ctcatccttc ctaaacatac cttgcccaga acaaggtgca
    17281 tctataataa ccttatcaaa atacccatta aacgtctttc ttaagctatt agaatcggtg
    17341 tttgttataa tacaatttct cgctccaaat cgctctaaat tttcccctag agccttaatt
    17401 ctactagcat taatttcatt agaaaccaat aaacctgtat cgtttagctt agatagtata
    17461 tatgtagatt ttccaccagg agcagcacac atatcgagaa ctttttcacc ctctttaata
    17521 tctgcctttg gtattacact cattgcagaa ggctcttgca aatagtatgc tccagcctca
    17581 tgcagtggat ttctaccagg tctgtcaatt ttatcatcat aataaaaacc ttcttctgcc
    17641 catggtattt gattcaaatc aaacaactcc aaatcaaata attttttctt gtcaattttt
    17701 aaagtattta ttctaagccc agtagtctta ctttcctcat aactttttat aaaatcatca
    17761 tattcatctt ttagtatttc ttcaatatct tctaaaaatt cctttggtaa ttctgtcaaa
    17821 tctataatcc cccttcaatc tgctttactt gtgtgtcatt tattatactt taattattta
    17881 tgtctttcct ttaaatttta tttcttcttc tttttcctaa gattaccgaa aaacactttt
    17941 ttagagtgat tcatatattt ctcaagttta tcaagattta tcttccacaa aattattaca
    18001 taaactataa gtaatataat agcccatcta tattctatca tcaatatgaa atcaaaaact
    18061 ccatgtaata aaattggaat taacactgcc ataaacaaat actcacgtct tttattttta
    18121 tttccctcaa atttatactt agaaatatag tagcccatag taattgcaaa cattacatga
    18181 gctggtatag agataactgc cctattaata ccaacttgca gtgctagatt tctactttca
    18241 gaaaaaatat aaaccatatt ttcaattgtt gcaaatccta aagctaaaaa tacagagtaa
    18301 ataataccat ctaacttttc agtaaagtgt ttttctttaa ttagggcagg aatcagtatc
    18361 agagctttta caccttcttc cacaagtcct gcaactacaa atgatacata tattagttct
    18421 gaataccctt caaatacatt aaacttcatc agtaaatttt ctaaaagtat aattataaca
    18481 cttagaaaag cacctattaa aaaaaattta attagatgga ttaggggttc tctacaatat
    18541 ctatctttag accttatcca aaacatccct atcaatatag gaactattgc taataaaaat
    18601 aaatctagct tcatagatta ttttcctttc aaaatttttg atttatttat gatttatatc
    18661 atctacttat ttttagctta tttgtgtttt aaatacatac taaaatatac attaaattat
    18721 tttaaattat ttaaatacaa atatactcaa atactcaaaa aaatttaaat ttatatctat
    18781 attatttaac aaatttcaag ctatattcat atcttgaaat ataagggaac taaattcatt
    18841 taaagaaagg gtgatttgat gcaagatggt ttcttaacag taagcataat tgatgctact
    18901 aacaatagac ctattcaaaa tgcagtagta aacatatatt caatgtctaa tggtagccaa
    18961 agctcatcaa ctttatacca aaacctaaga agtaatgaat ctggtcaagt gactggttta
    19021 gtattaccag caccagatgt agattactct ctacagccat ctgatgttag accttatagc
    19081 cagtatatag tggaagccat agcagatgga tatgagacag ttgtcataga aggaacgcag
    19141 ttgttagcta caattgtagc aagacaagga gttcccatgt ctcctagaac aagaagtaag
    19201 cgttctttct caagacaaag tgagttgatt tttgacatag gagaacatac tctatatggt
    19261 acttatcctc ctaagattcc agaaagtaac ttaaaaccat taccacctcc tactggtttc
    19321 gttgtattag ataaccctgt agtgcctgaa tttatagtag ttcatgatgg tttgccagaa
    19381 gattcatcag ctccaaacta ttggattcca tttaaagagt acattaaaaa catagcgtca
    19441 tctgaaatat attcaacttg gcctgaacag acaatctatg caaatgtaat agctataata
    19501 tcatttactt taaatagagt ctttactgaa tggtatagaa ataaaggata taactttaca
    19561 ataacttcaa caacagcata cgaccataag tttataaata atagaaactt atttgaacca
    19621 attaacgtag ttgtagatgc tatttttaat acttttataa aaagacctcc tacatcaaga
    19681 cagccactat tagcacaata ttgtgatgga caaaaatctc aatgtcctga ccaaatgact
    19741 caatggggca gtaaagactt aggtgaccaa ggatatgact atgagagtat cctaagatat
    19801 ttctatggag atgagatagt atttgaaaga gcacctatag taagtggtgt accagtgtca
    19861 ttccctggta ctacactaca agttggttct tctgggcagt atgttagaac catacaaaat
    19921 cagttaaatg ctatatctaa ttcttatcca gctgtaccaa aagttataga agatggtatc
    19981 tatggagcag atacagaaaa cgctgttaag atattccaag gaatctttgg tttacctcaa
    20041 agcggagtag tagattttaa aacatggtat gaaatatcca gagtttatgt agctacaact
    20101 agaatagctt ctttaaatcc tttaatttaa agcttgattt agaacataaa ctcaaattat
    20161 tgaataaaat tattaattta aaataaaaat ggctgttatg aactaatata taccataagt
    20221 atttttactt ataagtattg tagtcttaac agccattttt tagttataac ttgttttttt
    20281 agaatcttaa aactttaaat gggggatttt aagcttctta aaaacagtgc ttagcaactt
    20341 agatttcttt aaaatattat atttattttt aagtttaata aaatgctatt ctaacgaaaa
    20401 gcttactttt tatttaaaat ctcacttatt atttaaaatg ctacttctca cttagaatat
    20461 tactccttat tatttaaaac actatctatc acttagaata ttccttctta tctaaattat
    20521 tatttattat aaatataaat aatattaatt taaaaagtta ttcataattt aaataatact
    20581 attatcttca tcagtgaacc taaactgcaa ctttagactt attagctaaa gccaatatta
    20641 gacctaaagc tatagatgca aatgcagaag caattactct atactcagca gggaataaga
    20701 atacaattgt atgacatggt atccagaaaa atggtatagt ttttaaaaca ctaaaaccaa
    20761 ggtaagcatt ccagtccaca atatttacca catctttaag ttttacctta atcccctttc
    20821 caacctcata ttttttatct ataaaacagt ccataacctt atggaataaa aatacagttg
    20881 gtgacattag tatattcata gttgcactct taaatagagc atttgcaaaa gctattccct
    20941 caaatggaag cataccatcc acttgtgctt gacctacacc tgatgagtag attgacattc
    21001 catatgtggt aactacacct attattcccc aaactattgc tctataaaat actcctactg
    21061 gtattttcca atccttatac aaaattctag caccaagtaa atctcccatt gtagctaata
    21121 cagcaaactt taaaaaaccc ccaatatatg gatgtgcacc tgttgcttcc ataaatactt
    21181 ctctactact tggtattgct aagacagcaa taatacaaat aagaactcca ctccatataa
    21241 aatcgccttt tttcatattt ataaatacta tttaatatat aaatagttct cctttctatt
    21301 aaagatttat ttaatttaca atctatctac aattatttat taatcactta ttattagata
    21361 ttcattttcc atatttaaat ttattctatc cctttatcta tttattttct tactactcta
    21421 tccaccatta tctatatatt tattttacga ttactctttc accctaaccc tttaattatc
    21481 tatttttttc tgatattctt atctaccatc atccatatat ttattttacg attactcttt
    21541 caccctaacc ctttaattat ctattttttc tgatattctt atctaccatt atctatatat
    21601 ttattttacg attactcttt caccctaacc ctttaactat ctattttttt ctggtattct
    21661 tatctactat tatccgtata tttattttat gattactctt ttaacctacc ccctatactt
    21721 tatagtaata tgtttttaac ctttttcatt attcccaata tgctttataa ctacaaatac
    21781 tctcccccct taaaaataat aataaaatta atcaataaat ttataatcat attattactc
    21841 tatgtattaa gcaattacta tgccaataaa ttttgatttt tttaaacaaa tcttaaaaat
    21901 cctaaaatgc taattttctt taacttttaa tgaatctaat agataatatt taattttcgt
    21961 tatatttttc agaatgtttc aattattatt tttaagaaaa aaaaataatg ttaacatttc
    22021 ttaacataca ttaaaaaatg ttaacattat aaatttggtt taatactttt actttgactc
    22081 gatttacttt tattctactt cttactttat ttttaactta gactttattt ttttattact
    22141 tatttgagtc ttatttattt tattctcact ccatatgttc aattacattt gtctaaattc
    22201 tttgatactt ttattacata attttaacaa gcttaagtgt tatttgggta gtgcagttga
    22261 taattattta aataatcatc aactgcatgt ttatatcatt acagtagctt aatgtattta
    22321 atcatactta cttaggattt tgaaatatct tagagattat atttaacatt agtcaattgc
    22381 tgcataattg taagcaattg aaatttgaga cccatcttca ttttctatag tgataggctt
    22441 cataattttt aatccttcag tttttatctt ttctaattct tgttccattt tcttaattgt
    22501 ttcctcaagg tctttttgac ttacatcttt gactgcatta gggtctttag catagtcttt
    22561 taattcttga atatgtttag catactcttc ataagtataa tgctcaacac catacttttc
    22621 caattcagta gggtcagtta ttcctgattt tattagagat tcaagtttag gtaaagcaat
    22681 attagatgaa tcatttccat ttgcaaatac tgctccagtt cctcctagta ccattgcaga
    22741 taccatcatt gcacttataa gggatttttt tattttcata accaatcttc ctttcttaga
    22801 gatttatttg ggtagtgtag tttaaaatga ctattgcaat agttatcaac cacatcttta
    22861 gactaccata gtagtcatat ttgtgtcaag tctttttaaa aaacttttca ataaaaaata
    22921 ctatctcaaa acaaaattaa ttattttgag atagtatttt aattctaaat cttttatatt
    22981 actaagattt atggttactc tatccatgtt ctatattgtt ctttattaac tgctaacaaa
    23041 ttcccttttc ttgatatgac aaagcgatta tcattatagt tactttgctc aattgaaact
    23101 tctcctgaat cagtataaag tacatcctta ctattactag atttatttac atacgtttct
    23161 tcactattct cagaattagt tactttgctc atagatgatt cttcatgctc tctaaattca
    23221 attacagata cttttgccgt actaaaatga gtctctccat cacttttagc tttactaaca
    23281 agaatatcag attctacttt gggagaatat aaatcagttg atttactact tattgaaaat
    23341 ccacttttat cactttgttc attttttgtt aaaccaaaag attcaacctc attaatctct
    23401 ttattttctg ttgcaaaaac aagacctgtc ccacaaacta atacagttga aactaaaaaa
    23461 gtaacataag ttgatttttt aaatttcata atattaacta ttctcttttc tatttttgtc
    23521 tttccaaatc cactacatat tgtcattgaa aatggattat tctcctctgc tagacttatt
    23581 agtgtggttg cataatcagc cctatattct tcaccaagta tactcataac cttttcatca
    23641 caactaaatt ctatgtcttt atttagtaaa aaaaacatca cccaaactaa gggattaaac
    23701 caatggacaa caagagctac aagcataaat aactttaaca gattatcaaa gctattaata
    23761 tgaaccattt catgagttaa aacaaagtct aaagtttgat tattagcata attcatttgc
    23821 tttggcaaaa taattcgtgg ttttaaaata ccagatgtaa gtggtgtcct agtaaattca
    23881 gatatgcaaa caataaatct tctaaaacgg ctattcttac acatccattt tttaatgaac
    23941 tcatgatttt ttatatgaat tgaagtttta aaaagattaa ttattttaaa atatctaaag
    24001 cataaaagta tgctaaacat aatagtaact ataaaagaca aaatgcccaa catttccaac
    24061 atcataatat tttcaatatt agacattatt tcaaattcgt cagaattgtt tttacctgta
    24121 gagtcactag ctttcttaag agtataattt tctgttgatg tattacccaa ttctacatca
    24181 aaagtcgatt caattatact ataaatacta aaaccagaat ttaaacttac tggtattaga
    24241 agtttaaata aaacaatcaa ccacaataat acaaatacct ttttaggtaa ttttttaaat
    24301 aaacatgctc ttataaaaat aactattaaa agtaatactc cagaagtaaa actcatttct
    24361 aacaatttaa tgaacataaa tcttacctca ctttaatttt tcaacaattt gtttgagttt
    24421 tgtaatctca tcacttgaaa gattttcttt attaataaaa gctgaaagaa atttttctga
    24481 agaccctcca aacattttat taattaaagt ttctgtttcc tgtttttgta cttcctcttt
    24541 gcttattaaa gctttacaaa caaaatttgg ctcaattctc tctattgcac cttttgacac
    24601 taattttttt ataacggtat acgtagtgtt tctattccat cctgtttctt cttttaaaat
    24661 ttttactaac tctccagcct tgctttctcc tctctcccat agaacttcca ttactttaag
    24721 ttctgaatca aataatttta tctccatata aaatcgctcc tcaaatatag ttatcacaat
    24781 agtcattact ataataaaaa actatcatag tagtctatac tatgtcaatc atttattttt
    24841 taataataaa aactgtcttt ttaaaaaatt cttataaaag acagttttat caatgttata
    24901 ctttaatgtt taattaaagc catattttat ttaaatgtat aactatctat cttagtacat
    24961 agtataagat ttttctttat ctaatagtat atcctctaca tctgggtttt cttttactaa
    25021 ttgtctataa aaattacaat tagtaacacc tacatatgga attagccata gtagacctat
    25081 tccaagagta gctattgaaa gtatatacca tcctataaaa ctaaagttta gtacaaataa
    25141 tttaagtaca tttcctttca tcatatctaa actagcaccc attgatttaa atacacccat
    25201 attatccttg tcttcacaaa ttaagaaagt agcttgagaa acatataata gcaatatata
    25261 aaccggtaac ataagaacaa gtattataag aactagagtt attccagtag aaataatagt
    25321 ccctattcca acattatcac ttgataaata ttcaattgct ccactagtaa gtaaaactcc
    25381 aaatgataaa aacgctccta taattgtgat tataaaagaa taaatataaa caataattga
    25441 ttcaaataaa taaaaaccaa aacttcttaa ataaattctc ccacttacaa agatatctcc
    25501 tatcttgact ttttcatctt tagtaatttt caagaacata catgtaaatc ctgctgaaac
    25561 gcaaaaagct attacagcca ctacaatagt agtcaatggt tcaaaaggta atcctacaat
    25621 tgatattaat actatcaatg caatatatac tgccatagaa attgcacata ttccccagtt
    25681 tccagataac tgagctttag atactcgttt taattcttgc cttgatacca taaaaaacac
    25741 ccctttcatt ttataaaaaa acttttaaaa atataaataa tcttttaacg tataatatta
    25801 atatatcata ttttcgacta tataagatac aattattcac catgttttat caagatatgc
    25861 cataattgta ttactagttt tatatagtca acattaccaa atacatcaaa taaatttgat
    25921 ta
//
LOCUS       DAMKPG010000055        25912 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMKPG010000055 DAMKPG010000000
VERSION     DAMKPG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.058"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            63..1352
                     /locus_tag="PC621_002289"
     CDS             63..1352
                     /locus_tag="PC621_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF4109439.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            1381..2061
                     /locus_tag="PC621_002290"
     CDS             1381..2061
                     /locus_tag="PC621_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF4109440.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            2162..2701
                     /locus_tag="PC621_002291"
     CDS             2162..2701
                     /locus_tag="PC621_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF4109441.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            2877..3896
                     /locus_tag="PC621_002292"
     CDS             2877..3896
                     /locus_tag="PC621_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF4109442.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            4184..5374
                     /gene="dltD"
                     /locus_tag="PC621_002293"
                     /pseudo
     CDS             4184..5374
                     /gene="dltD"
                     /locus_tag="PC621_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
     gene            5495..7009
                     /locus_tag="PC621_002294"
     CDS             5495..7009
                     /locus_tag="PC621_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF4109443.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLREEI"
     gene            7009..8157
                     /gene="dltB"
                     /locus_tag="PC621_002295"
     CDS             7009..8157
                     /gene="dltB"
                     /locus_tag="PC621_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF4109444.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            8176..8406
                     /gene="dltC"
                     /locus_tag="PC621_002296"
     CDS             8176..8406
                     /gene="dltC"
                     /locus_tag="PC621_002296"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF4109445.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            8623..9408
                     /locus_tag="PC621_002297"
     CDS             8623..9408
                     /locus_tag="PC621_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF4109446.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            9687..11597
                     /gene="phnW"
                     /locus_tag="PC621_002298"
     CDS             9687..11597
                     /gene="phnW"
                     /locus_tag="PC621_002298"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF4109447.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            11954..12775
                     /locus_tag="PC621_002299"
     CDS             11954..12775
                     /locus_tag="PC621_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109448.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            12816..13583
                     /locus_tag="PC621_002300"
     CDS             12816..13583
                     /locus_tag="PC621_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF4109449.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            13591..14211
                     /locus_tag="PC621_002301"
     CDS             13591..14211
                     /locus_tag="PC621_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF4109450.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(14511..15098)
                     /locus_tag="PC621_002302"
     CDS             complement(14511..15098)
                     /locus_tag="PC621_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF4109451.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(15184..15621)
                     /locus_tag="PC621_002303"
     CDS             complement(15184..15621)
                     /locus_tag="PC621_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4109452.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            15725..16723
                     /locus_tag="PC621_002304"
     CDS             15725..16723
                     /locus_tag="PC621_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF4109453.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(16843..17769)
                     /locus_tag="PC621_002305"
     CDS             complement(16843..17769)
                     /locus_tag="PC621_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF4109454.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            18155..19759
                     /locus_tag="PC621_002306"
     CDS             18155..19759
                     /locus_tag="PC621_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4109455.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            20576..21847
                     /gene="serS"
                     /locus_tag="PC621_002307"
     CDS             20576..21847
                     /gene="serS"
                     /locus_tag="PC621_002307"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF4109456.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            22126..23631
                     /locus_tag="PC621_002308"
     CDS             22126..23631
                     /locus_tag="PC621_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF4109457.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            23745..24443
                     /locus_tag="PC621_002309"
     CDS             23745..24443
                     /locus_tag="PC621_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF4109458.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            24728..25816
                     /locus_tag="PC621_002310"
     CDS             24728..25816
                     /locus_tag="PC621_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109459.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT        10289 a         2651 c         4433 g         8539 t
ORIGIN      
        1 taaatataag gggggaactt tgagttctta tagatttctt acattataag aaccaatact
       61 ttatgcggga gttattaaaa gtaacactta tagtaatgct tgttttgata aatgcttttt
      121 ttgtagcttg tgaatttgct atggtaaaac ttcgtagttc taggattgat accatgataa
      181 ctgaaggtaa taataatgca aaaagatgta aaataataaa agacaactta aattcctatc
      241 tttctgcatg tcaacttggt ataacacttt gttcacttgc attaggttgg atgggagaat
      301 ctacagttaa agagttaatt ttaccaatag taagtgtatt taatcttagt gaaagtatta
      361 tatatactat atcaatttcc atttcttttt taattataac aatgatggag gttgtaatag
      421 gagagttggt tcctaaagct ttagcacttt ataatacaga aaaaataatg ttgagtacat
      481 cttttttatt agtaggattt tataagttaa catatccaat aatatatttt ttcaacttaa
      541 gtactgattt atttcttaaa ccatttggat attcacaagc tgatgaggtt gatgaacccc
      601 atacaggtga cgaaataaga ctattagtag aagaaagtta taaaagtgga cttatagatg
      661 aatcagagca aagattagtt gataatattt ttgaatttga agaaaagaaa attagagaga
      721 taatggttcc tagaactgat atggtatgta tatatgaaag tgattctgaa gaaaagatat
      781 tagctatttt aaaagaggaa ggtgttacaa gatatccagt atgcagaaaa aataaagatg
      841 atatattagg ttttgtacat ataagagatt tatacaatca aaagataaat gaaaataaaa
      901 ttgaactaga agaaatattg agagatatta tatatatatc tgaaaattta actatagata
      961 aagctcttga gagaataaga aaggaaaaac ttcaacttgc tatagttgtg gatgaatatg
     1021 gtggtacatc tggtgtggta acaatagaag atatactaga agagatagtt ggagaaattc
     1081 aagatgaatt tgacaaagaa gagactcata ttaagaaaat aggagcaggt tcttacttag
     1141 ttgatggaac tgaatctatc aactatatta ataagtattt tggacttgat atagagcatg
     1201 atggatttga cagtataggt gggtggatat cctatatgct tggttcaaat attaaagtaa
     1261 accaaagcgt aacttttgaa aattacaatt ttacaatatt agaattggat aaattgaggg
     1321 ttgtaaaatt aatcataaaa agtgtaatat aattatatca aaaaatattg gaggaattgt
     1381 atgcaaaaag tagaaggaat tatttttgac atggatggag ttttgtttga ctcagagaga
     1441 atttctcttg aattttggat ggagacgttt gaaaagtatg gatatactat gactaaggaa
     1501 atttatacat cagttatggg aagaaatcgt aaaggaataa tagaaggtct tacagatata
     1561 tacgatagtt ctgtaccaat aatagattta tatgacgaaa aaactaaaaa tatgattgaa
     1621 tttatggaga ggaagggagc tcctatcaaa ttaggagtca atgaactaat ctctttttta
     1681 aaggaaaatg gatacaaaat ggctgttgca acatcaacaa agagagaaag agcagtaaag
     1741 agattagcta aggcaaattt aaaagattac tttgatgcta tagtttgtgg agatgatgtt
     1801 gtaaactcta aaccaaaccc tgagatattc ttaaaagcag ctaaaaagat aaatgttaat
     1861 ccccaaaatt gtattgtcat agaggattcg cctatgggag ttgaagctgc atacaatggt
     1921 gggataagat gtataaatgt accagattta aaagagccag atgagcaaat taaatcacaa
     1981 tcacataaga tacttgaaaa tttactggaa gttagagaat acctaaaaag tttgaactct
     2041 aaagaatgtc acaatctata atataaagta ttaattaaat aaattaaata taatttcttt
     2101 gatttagaat aaatccatga atatgagaaa aaataagtat aaacagctag gaggaatatt
     2161 catggataat gtaggttctc tattatattt tttatttaat tttttcttgt atggttttat
     2221 aggttggata attgaaaact tatatagtta ttataaacaa gggcattttc aagaagatgg
     2281 atttttaaat ggtccattta aaccaatgta tgcaattgca atgtcgatta taatagaggt
     2341 ctcaagattt attgacccca ataatatatt tgcattgctt ttattatgtc tagtagtgcc
     2401 gacagtagta gaatatatta caggaatact tatgaaagta tattttaata aggtctattg
     2461 ggattattca gattataagt acaatagtag aggaatagta tgtctaaagt tttctgtata
     2521 ttggactata ttgacattta ttggagttag atattttcaa gtgtatatag ttgatagttt
     2581 atatagtctt ataatacctt attggccaat tcttgtaact atacttttag taacattgtt
     2641 tgtggatgaa attcttacta taaaattttt gaggggaaat aaagatatag tagaaagata
     2701 actttaaaaa tataagttga atttagattg tagttaaaag aaagctatgt taaaatggtg
     2761 aaatcattta aaattagctt tcttttttat ttgataaaat tattttataa tatatctata
     2821 agttgtaaag ttttttatta aaattaaaat aaagtgtaga aaatggggag tgatttatga
     2881 tgggtaaaat aagatttggt attgttggga caagcaatat agcaaatata tttctaagag
     2941 ctgcatctag ggttaaagat tttgaattgg ttgctgtata ttctagaaat ttagaaaaag
     3001 ctagagaatt tggaactcta catggagcaa gtattttttt tgatgattta gaacaaatgg
     3061 ctaaatcgaa ggatatagat gctgtatata tagcttctcc aaatgcatta cattcaaagc
     3121 aagccattac ttgtttaaaa cacaaaaaac atgtgctttg tgaaaagtcg ttagcatcta
     3181 atttaaaaga agtcaaatca atgataaaaa cagccaaaga taataatgta ttgcttatgg
     3241 aagctatgag aattacatgt gttccaaact ttaaagcagt taaagaaaac ttgtataaaa
     3301 tagggaaaat aagaagattt tttggaagct attgtcagta ttcttcaaga tatgataaat
     3361 ataaaaatgg aattatagaa aatgccttta aaaaagagtt gtcaaatgga gctttaatgg
     3421 atataggagt gtattgtata catcctatgg tgaatttatt tggtgctcca aaaactgtaa
     3481 aggcagtttc tcatatactt caaactggag tggatggaga aggttcagct gtatttcaat
     3541 atgatgatat ggatgcaata attcagtatt ctaaagtagc tgattcttat ataccatctg
     3601 aaatacaagg agaagaggga agtataataa ttgaaaagct taatctattt gaaaaagcta
     3661 ttattaaata cagagatgga agagaagaag atatatctat tccaaaagaa aaagcagagg
     3721 aaaagcctag agaaatagaa ggaatgtatt atgaacttgt agagtttata agtcttataa
     3781 ataacaataa aatagaatca aatataaatt ctcatgaaaa ttcaataata gtaatggaaa
     3841 ttatggatga gataagaaga caaagtagta ttgtttatcc agctgatagt atatagctta
     3901 tattagaaaa tataagaatt tataatatat attcttaaat aaatttaatt ttaagttaag
     3961 gtaaggtgta tacttagcca agtgttaaaa tgtcaaatta taagtatgaa aaagtgctaa
     4021 aaagaaattt atttttggtg tttttttctt tttttttaca attttgtaac agtatcaaaa
     4081 aaagttttga cgattttatt acaattttgt agttgaatat acatatcaaa actaatatga
     4141 ttaataataa cataaatttg aatattttta gaggagagaa aatatgagaa aattgatata
     4201 ctttattaca ccttttataa taggcgtagt atttttattt gggttagaca aatttcttga
     4261 ttctaaaact gatgaacttt tgagggagaa aaatctactt ccaattatgg atgatacttt
     4321 aagtgatata aaagataaag gggttacagc aaataatcat tttttaagag aaaaggatat
     4381 tatgattttg gggtcatcag aacttagtaa ttcaacaaaa caacatccaa agtactattt
     4441 taatacaaat aggagtaaaa ataaggtgtt tgcaattggg agagcctata ctcagacatt
     4501 acaagatgct gcaatattag gaagtatgaa tcctaatatt gataataaga aagttgtctt
     4561 gttaatttct atgcaatggt ttatggaaaa agacggagtc acaagtcacc attatcagtc
     4621 tagattttca ccaatataat tttatcgatt tttagataat ccaaaaatat ctaaacaaaa
     4681 taagattgaa tatgcaaaaa aatcaagtaa gctattatgg ggaagtgatg agtataaagc
     4741 agaagcatta tatgctaagc tatatgaacc taagacattt cctgaaaaag ctgaaaaggt
     4801 gcttttagag ccttattttc aaggaagaaa gtactgtata gctcttaaag aaaagggtat
     4861 tctttataaa cgattaatta agctagataa aaagagagca acaaaaagaa aaagaccaat
     4921 aaattggtca tatgagagaa agaaagccat tgaagatgct aaaaaaagag ttggtaaaaa
     4981 tccactaaat attgataagt actattataa agagcatttt aaagatggta tagagcaata
     5041 caaaggaaga gacaaagatg taaatttatt aacatctaag gagtttgaaa attataaact
     5101 gatgctaaat gtatgtactg acttaggtat taatccagtt gttgtattaa tcccaagtat
     5161 ggataaattc tataatttga ctggtatttc tgaaaaagaa agaaatcagt actacgataa
     5221 agcacaaaaa atagcagaaa gcaaaggctt tgaagttcta aaccttaaag ataaagggtc
     5281 agataagtat tatttaaggg atgtaatgca tcttggtacg aaaggatggg tagatgtctg
     5341 tgagagatta tttaaaatat ttaaagaaca ataaagattt gttaactagt atttgtttaa
     5401 ctatggttat aatattggcg ataggagttc taacatttac tccatttaaa gaattgccat
     5461 ttatttataa cggattttaa aggagcattc ttatatgaaa attattgaag gaataaaaaa
     5521 atattcaaat acagatagaa cagcactaat gtgtaatgga gataaacttt cttataaaga
     5581 tttaaatgaa tattcagatg ctatatcagt atttctaaag gatgtataca aagaagagga
     5641 tactcctata gtaatttatg gaaataaaga aaacatgata atggcatgta tgataggagc
     5701 tttaaaatca ggtagagcgt atgtgcctct tgatattagt tttccaattg atagagtttt
     5761 tgaagtcact aaagagataa aaccaaaagt aatatttaat tttagtgatg aaagaaattt
     5821 tggagatatc aatgtaattg atatggataa attaaatgat ataataaatg aatatcaggg
     5881 aaaatcatta gataaagaaa actgggtaaa agatgatgaa aatgcatata tactatttac
     5941 ttcaggaagt acaggaaagc caaaaggggt tcaaataagt tcaaacaatt tagatagctt
     6001 ttcagattgg ataagcccat atctaaacat agatggaagt gaaaaagtaa taatgaacca
     6061 accagcatat tcttttgacc tttcagttac aacaatatac cctggtctta tccatggagc
     6121 tacattattt tcaatatcaa aagatgtatt agcagactat aaagaactat ttagacaatt
     6181 tagtatctca gatatagctg tatgggtgtc aactccttct tttgcaggag tatgtataac
     6241 tgaaaaagag tttaatagta aaatgcttcc taaccttgag tcaatgatat tcataggaga
     6301 agcactttca aaaaatctta ctaaagaact tatgagtaga tttccaaaca caagaattat
     6361 caatggatat ggaccaaccg aggctactgt tggtgtaagt gtaaatgata tgactcaaaa
     6421 agctatagat gatgaaaaaa gtcttccagt tggataccct atgtcaaatt gtaaaataaa
     6481 aatacttgat gaagatggaa atgaacttaa agaaaatgaa aagggtgaaa ttataataat
     6541 tggaccatca gtttctaaag gttacttcaa taataaagaa aagactgatg aagtattttt
     6601 ctatgatgaa atagatggtg taaagtggag agcttataaa actggagata tgggatatct
     6661 tttagatgga aatatatatt attgtggaag aaaagatttt cagattaaat taaatggatt
     6721 tagaattgaa atagaagata tagaaaataa tttgagaaaa gtacataatg taaaaaatgc
     6781 agtagtttta ccagtatata aggacgaaaa aattgcttac ttaaaaggta ttgttgagtt
     6841 aaatgaaaag aatgatttaa gtaatattaa aaatgggatg ataataaaaa aggaacttgg
     6901 aaagtatata ccttcatata tgattccaag aaatatttca ataatctctg agtttccaac
     6961 aaatatcaat ggtaaaatag acagaaagaa acttagggag gaaatttaat gattttttca
     7021 cagtatggag attattttta tctttatata ttattattaa catcaatacc agcagtaatt
     7081 ttaggtctaa tgggcaagaa tataaagtat tatggtatgt tggcaagttt gtttatgata
     7141 ttcttaatag taggaattga tgtccagcta aagtatttgg taatatttat tattcttgaa
     7201 gtaataatag ttaaaggata tgaatatgta agaagaaaaa ctaaaaataa atacatatat
     7261 tggggattct tgtttgcatc aatgctccca ataataataa ataaaatatc tccagttact
     7321 tcttttggaa taattggatt tattggaata tcatacttaa atttcagaac tatacaaatg
     7381 gttattgaaa tttatgatgg agctataaaa gaagttaaaa tttcaaagat gctttatttt
     7441 atgttatttt ttccaacttt aagttctgga cctattgata ggtcaagaag atttgaacag
     7501 gacttggaaa aacacatctc tagaaaagag tatatagaag aatacttact gccaggaata
     7561 aaaaatattg tcatgggagt aggttataag tttgttatag catttttgat aaatacatat
     7621 tgggtatcaa gaatacctaa agatatgagt tttataaata ttttaagtta tatgtacgca
     7681 tatagtttat acctgttctt tgattttgca ggatatagtt tatttgctat aggtacaggg
     7741 tacatatttg gaatacaagt acctataaac tttgacaagc catttatcag taaagatatg
     7801 aaagagttct ggacaagatg gcatataagt ctttcaagat ggtttggaga ttatattttt
     7861 tctagatttg ttatgtcatc aatgagaaag aaaagattta agaaaaggac tactgctgct
     7921 catgtggctc agatgataac catgataaca atgggatttt ggcatggtct aacttggtat
     7981 tatgttgctt atggtgtata ccaaggattg gcacttgtgt taactgatat atatcagcga
     8041 aaatctaaat tttataaaaa acataaaaaa gataaatggt tcgagagagt gcagattttc
     8101 attacattcc atattgtgtg ttttggattg ctgatattct caggttattt agcttaattt
     8161 taaggaggaa aatttatgca agaaacagtt atagaaatat ttgaagatgt attagggact
     8221 gatgagataa gagaagattt agaccttaat ttatttgaga cggaactttt agactcatta
     8281 gctataattg aagttttact tgaaattgaa aatagattag ggatagagct tcaaccaact
     8341 gatttagaga gaaaagatat gtctactgta aataatttag taaaattctt ggagacaaga
     8401 aaataaatta gtagttagac tttaaatatt aatttataaa ataaaatttt acaaagtcca
     8461 tctgatacta atatttgatg gactttttta atgataatac aaagtctttt gaaaataact
     8521 ttaattaagt aaaaaattta atacataaat atgatttcat aaatcttagg tatcattgta
     8581 atattatagg agaaaaatat attatattta ggaggaaaat gaatgtcttt aaattcgata
     8641 gatagaaaaa aagaaatact taaattatta gattcaaaag ggaaggtaaa tactaaagaa
     8701 ttagtaaaaa gattagaagt atcatcagaa acaataagaa gatatttgga agaattagaa
     8761 aatgaaaata aattaaaaaa ggtatatggt ggtgctataa aaataaatta tggagtgatt
     8821 gaaccagaac atataaagag aaacaatact aacctagata aaaaacaaag tattgctaaa
     8881 tacgcagcta agtttgtaaa tgacaatgag tcaattatca tagatgaagg tactaccaat
     8941 ttgcagattg tagattatat aatatctgta aacaatttaa aaattattac aaactcttat
     9001 ccagttctat caaaattgat ttcttatgag aatcaaggtt tgtttgatgg agaagttata
     9061 tttattggtg gaaaagtaaa tttaaaacac caaaggacag catgtccaat gtcaatagac
     9121 actatgaaaa acttatacgt agataaagct tttatatcta gtgaaggtat acttgatagt
     9181 tttggaatta gtagtataga ccaaaataag gcattgttgt caaaagaata tataaaaaat
     9241 tcaaaggaaa gttttgtatt atgtgatagt tctaaaatag gaattgctag tatgtataag
     9301 atagcagatt tagaggaatt agattttgtt atttgtgatg tagaacctcc gaatgaatgg
     9361 aaggataagt taaatgagat aaatataaca tggatagaag caaaataaac ccttagtttt
     9421 atataataca actatgctta atacaactaa attcataaaa tatagtcagt tcatagatta
     9481 agattagaaa gtaaatttta gattgtaact taggatttta tggaaatatg atataaaata
     9541 aaaacaatta aatacaacta aaaacagaaa tttaatatat atttaaccta aatatgatgt
     9601 ataattaaca tatgaaagtt atactttaaa agtaaaaaca attaaaaaca caaaaattct
     9661 aaaatgaatc tatggaggct ataaatatga aaaagatata tggagaaaaa ataaaagcag
     9721 tagtattcga ttgggctgga acaacagtag attatggatg ttttgcacct cttaatgtat
     9781 ttatagagat atttaaaaga agaggtatag atgtaacaat ggaagaagca agaaaaccta
     9841 tgggaaaatt aaaaatagac catataagag aaatgtgtga aatggataga ataaaaaatc
     9901 tttggtcaga taagtttggt aaagtgccaa ctgaagatga tgtaaatgaa ctttatgctg
     9961 aatttgagcc aatgttattt gagacattag aagagtatac gactccaatt cctcatgttg
    10021 ttgaaactat tgaaaagttg agaaaaaatg gattaaaaat aggttctaca acgggttata
    10081 caagagagat gatgaacata gttgaaccaa atgcagcaaa gaaaggatac tcacccgatt
    10141 ttcttgtaac accatcagaa gtatctcaag gaagaccgta tccttggatg tgctataaaa
    10201 atgctgaagc tttaggggtg tctcctatga gttctatggt aaaagtagga gatacaataa
    10261 gtgatgtaaa agaaggcgta aatgcaggaa tgtggagtgt tgctgtaata aaaggttcaa
    10321 gtgagttagg gcttacacaa gaagaagtag agaacatgga taaggaagaa ttaaaagcta
    10381 agatgagtat agtttcaaag aaatttaaag aggcaggtgc acattttgta atagaaacta
    10441 tggctgagtt agaagatata ctaataaaaa tagagaatga aactattaaa tcagattttg
    10501 taccagaaaa tgattacata ttacttacac caggaccact atctactact aaatcagtaa
    10561 gagcatctat gttaaaagat tggtgtactt gggatgttga atataataac ctagtacaag
    10621 atgttagaag aagattagta tctctagcaa cccaaaatac agataaatac acaagtgtat
    10681 taatgcaagg aagtgggact ttttcagtag aagctataat aggaagtact atttcaaaag
    10741 atggtaaact tcttgtaatt gcaaatggag catatggaaa gagaatgaaa gatatatgta
    10801 attatcttaa catagaattt gttgactgca catttaaaga tatagaagca gtggatttaa
    10861 atgtagtaga aaatttatta aaagaaaaca aggatataac acatatatca atggttcact
    10921 gtgaaactac tacaggtaga ttaaatccta tacaagaggt agggaaactt gcaaaagaat
    10981 ataacaaaat ttatatagtt gatgctatga gtagttttgg gggaattgag attgatgttg
    11041 aagattttaa tatagatttt ttagttagta gttctaacaa atgtatacaa ggagtacctg
    11101 gatttggttt tataatagct aataaagaga aattatctaa atgtaaaggt atagctaaat
    11161 cattatctct tgatgtgtat gcgcaatggg agactatgga gaaaaataat ggtaaatgga
    11221 gatttacttc acctacacat gttgtaagag ctttttatca agctttatta gaattggaag
    11281 aagaaggttc tgttgaaaaa agatatgcta gatataaaga aaatcagttt acaatagctt
    11341 caagattaaa atctttagga tttgatactt tggtgaatga taatgctcaa tcacctgtaa
    11401 taacaacttt cttatatcct aaaaatgcaa agtttgaatt tatggaattt tatacttatt
    11461 taaaagataa tggatttgtt atctatccag gtaaattgac tgatatagat acatttagaa
    11521 taggtagcat aggtgaagtt tatccaactg atatggagag actagctgat gtaatagaaa
    11581 aatttatcaa tagatagaag aagatagtgt cttttaggtt acagaatagt gcggagagcc
    11641 tactccttta ggtgtgaaat gatagcacaa acagtgtata aaaagtattg acagtagaat
    11701 ttaaagactt atattttaga agtttataca atgaatatat aaagattgag taatagggat
    11761 atattttatt taaaccacaa tctaagaaat tagatttcta gtttaaataa agcatttccc
    11821 tattattttt atgcctattt atagaaaata aatgatagtt gctttattaa gtattattat
    11881 cagacttgac aacgtaacaa tgttttgtta tattaggttt aaagataaaa tacggtaaag
    11941 aaaggatatc attatgaatg aagaattaag ttcactaaga tttgatggtg ttggaaaatg
    12001 taaaggtgga atttttgata atgttaatat atcaggaaca ggcaaaatag aaggaagtgt
    12061 aaaatgtagt aaatttgatt cttctggttc agcaaaagtt tttgggacaa cagaatgtaa
    12121 tgaatttttt acatctggtt taagtaaagt agaaggaaac atactggcat caaaagttga
    12181 gatatcggga cttcttagat gtacaggaaa tattaattct gttgaaattg atactagtgg
    12241 aattattaat gtagaaggaa gtataaaatc ggatattatt catggagaag gatatttaaa
    12301 agcaaatgag aatatagatt gtgaaaatat caatttaagt ggaatcttgg agtgtaatgg
    12361 atttttgaat tgtgaagaag ttaatattag tttaaatgga acttctaatt ttaatgaagt
    12421 tggagcaagt agtataaata taaaaaaggg gcatggagat tattgcaaaa aatttcgtat
    12481 gctagaaaaa aagcatatca actttgaaaa agtgtcttca aatttaataa gtttaggtct
    12541 attatcaatt agaaagtata cagaaagtaa attgtttgca aatattatag aagcagatga
    12601 aataagctta gaaaatagtg aagtcaaagt tgtaagagga aaaaatatta aaataggcaa
    12661 gggatgttat gtagaaagtt tggagtacag tgaaagtata gagattgatg aagattcaat
    12721 agttggtgaa ataaagaata taagtgaaaa aataaaatta aagaaggata agtaataaat
    12781 aggtttaaaa ataaaattta aaatgaggta ataatatgga aaaagggtct ataaaaatac
    12841 ttggagaagg ctttataagt agtggtgagt atgaaaatat aaaaatcgtg ggaagttcta
    12901 aatcaaaagg aaatgttaaa agtaacgaag taaaagtcct tggagatgca aaatttgaag
    12961 gtgacatcaa tataaaaaat tttgttgtta acggaaaaac tattataaga ggcaatctta
    13021 aagcagagcg tgtaagagtg aatggtcaac ttaatataga gggagatgca gaaatagatg
    13081 acttagttat aactgctcaa cagaaagtgg atggcaaact taaatgtaat aatgttacaa
    13141 taagaggaca attaagtgta aatgaaaata tgtatgcaca agatataaaa atctatggcg
    13201 aaatgagaaa taaatcaaat atagaatgtg aagatataaa ggtatatgga gtaataaaat
    13261 gtgatggtct tttaaatgga gaaaatatat atgtttattc aggaggaggt tcttattgta
    13321 aagagatagg ggcaactaat ataaaaatag gaagaagcaa agatgctttt tcattaatta
    13381 aaatcattaa tattttttca ggaaagttta aatgtaattt gatagaggga gatactattg
    13441 aattagaaga tgcatcaata aaagatatac gaggcaaaag catatctcta gtagataatt
    13501 gtgacgttgg aaatattgaa tacagtgata atttatcagt ttcagataat tctactgtaa
    13561 aaaacagtat aaaggtgggc tagaaaagtt atggaagaat taatatctaa gaaagattta
    13621 ttgagcaaaa ctaatatatc ttatggtcaa ttatatagat ggaaaagaaa aaacataata
    13681 ccagaggaat ggtttataaa aaaatcagct tttacaggtc aagagacttt ctttccaaga
    13741 gataaaatac tagaaagaat agagttaata ttgtctatga aagaagagac ttctcttgat
    13801 gatattgcaa atatgtttat gaaaaaggat attaataagg aatttgacat tgattttatt
    13861 ctcagtaaag aagttatatg taattatacc aaagaaatat ttcaaaattt atataaaaaa
    13921 gagcaaagca taggtaaaaa agaactgctt atattgagca taatagagaa gtttttagtt
    13981 aaatctataa taacttttga agagttaaaa ttgatagtga atataataga agaaaatttt
    14041 acatcaatat acaatgaaag tggaaagatt tatttgttta gaaagtttgg agttccattc
    14101 gtagtaggat gtctggatta taaaaaagta aagtttgaaa aagatgtagt taagattatt
    14161 gagattgact tgacaaaaga aataaatgat ataagtataa agttatcata gtgtattatc
    14221 ttatataatg tattataata atctacttaa aatacacttt atttaaatat ttagtttaaa
    14281 gataagattt tatgtgagta tgtaaaagtt aaagatagat ttaagtaggt tatagggatt
    14341 agtaaataat aaaatgaacc atgatgaatt actataaatt gtatttaaac tacattttct
    14401 acttatagta atatcaatgg ttcattttat tgttattaag ataatacagt atttataaaa
    14461 atacttgata taaatatagt aaattgttta aatagaatta ttgccttagt ttattcaaat
    14521 aatgagtttt taaaatctaa gaaatatcct tgtggatgaa gacatacagg acaagctttt
    14581 ggagcttctt tacctatatg tatatggcca caatgtgtac atatccattg tatatcttca
    14641 tttcttctga ataaagtacc ttcttctaaa tcagtagcat atctaccaaa tctatcacca
    14701 tgaacttttt ctatttcagc tatattttca aataacttag ctatagattc aaatccttct
    14761 tcacgtgctg tttttccaaa gtttttatat acatcatccc actcttgaag ctcattatgt
    14821 tgtgcagctt taaggtgttg tgctgtcttg tcatataaat ctacagggta tgctcctgat
    14881 atacttatat tttcaccagc aaattctttt aatttagaaa agaattgttt tgcatgtgat
    14941 ttttcttggt cagcagtgta attaaataaa gactctatta tatataatcc ttcttttttt
    15001 gctacagatg cagaaatatt gtatctattt ctagcttgac tctcacctgc aaaagctctt
    15061 aataaatttt ctttagtttt actatctttt aaattcatat tctacctctc tatcataata
    15121 aatttttaca atatttgatt ctttttattt attattccaa taggagatat aattatgctc
    15181 aatttagtca gcatttttaa aattatcatt aatttttttt aaaattctaa gaaattcttg
    15241 tttttcattt tctgtaatat tatggtatgc tgtctcttta acttgtgttg agatgctatt
    15301 aaaagtatct tctataagca gtgccttttt tgtaagcttt atatacgtga tacgtttgtc
    15361 atttgtacat ttttgtcttt ctaaatatcc taggtttata agtttattaa ccaacactgt
    15421 aacagtggat ttatcttttc ctattttagc tgcaatttct ttcattgtaa gtattccatt
    15481 attttcataa agtgcagtta aaatatttcc atgagtaggt attaactcac ctaaaccatt
    15541 tttactgagt ttattttcaa tgaatttaat catactagcc tttgttttac ttatgaaatg
    15601 tacaatatat ttatcttcca taaattattc tccaatcttt aatctattta aattagtttg
    15661 acataaaact atttataaac tataattagt ttaacataaa actaaatgta ttgggaggaa
    15721 atttatgaat aaaaaattta aatcactata tattacagaa gaagaaggaa attttagaag
    15781 aaatatagtt gaaagagaag tagatacatt gccagaaggc gaagttctaa taaaagtaaa
    15841 atattcttct ctaaattaca aggatgcact ttctgcaaat ggtaataagg gtgttacgag
    15901 aaagtatcct catacaccag gaattgatgc atctggtata gtagaatcta gtagttgtga
    15961 tgattttaaa attggagaag aagttttagt aacaggttat gacttgggaa tgaatacttc
    16021 tggtggtttt tcagaataca taagagttcc ttctagttgg gtagttaaac ttcctaaaaa
    16081 tctttcacta aaagaagcaa tggattatgg tacagcaggg tttacagcag ctttatctgt
    16141 gtataaattt atagattctg tagacaagaa tatgggagat gtattagtca ctggtggtac
    16201 tggaggagtt gctgtaattg caactaaaat attgattaaa ttaggttatt cagttgtagt
    16261 ttcaacagga aaattagaag aacaaaaaga agtattacta aacttaggtg ttaaagatgt
    16321 tatacatagg tcagaagtgg atgataactc aggaagacct ctgcttagac caagatgggc
    16381 tggagtaata gatacagtag gaggaaatac tctagcaaca gcaataaaga caactaatta
    16441 ttgtggagca gtaacaactt gtggtaatgc aggaggagtt gattttacaa gttcagtata
    16501 tccatttatc ttaaagggaa ttactttata tggtatagat tctgtggatt gcccaatgaa
    16561 cttaagatta aaaatatggg atttattatc aaatgaatgg aaattagatg atttagatga
    16621 tatggtaaat gtagtatcat tggaagagtt aaatgatagt atagattcga taatgacagg
    16681 aaaacatgtt ggaagaacag tagttgattt agaaaaaata taggcgaata aatttataat
    16741 taggattata ggttttaagc aaaaatgaga tacttgaaat ttacttatga gtatctcatt
    16801 tttttatgta aaaaaattta tgtagatgtt tgtgtaaaaa gattattcat gaacaattaa
    16861 ttcaaataca tctggtcttg aatagtgacc ttctgaatca aaatctagtc tactaagtac
    16921 tattttttct aggtctaaat cagctattag catttcttct ttattgaaaa taggacctgc
    16981 tacatatttt ccaaaagggt caactataca acttcctcct ggacacataa tttctggttc
    17041 tgcatctagc tccttatagt agttaaggtc agttggatac atattttttt caacatattg
    17101 attgcatcca attacaaaac atcttccttc taaagcaata tgcttcatag ttgcttgcca
    17161 ctcttctctt gaatctgcag taggggctat gtaaagtttt acacccttag cataaagaac
    17221 tgttctagct aatggcatat aattttccca acaaatcaac gaacccattt ttccatatgg
    17281 agtatcaact actgtaagag tacttccatc tccttctccc caaatacatc tttctgttcc
    17341 tgtaggttta agttttctgt gtttaccaat tagcttacct tctggagaga aaaataagtt
    17401 tgtgcaataa agggtacaat ttatatcatt accatctctt tctgttatac ccattgatag
    17461 atatactcct gcttcttgtg ctgcttttcc taataaatct gttgtagcac taggaactgg
    17521 tacagaatta tcatagtatc ttttccaatc ttctctacct tccattgttc tacttccaac
    17581 aacaaatcca aatgaaagac ctcttggata tgcaggtatg aatgcttcag ggaaaactac
    17641 gatatttgcc ccttttctac cagcttcttt aattaatcct aatgcttttt caacagtttt
    17701 ttctaaatcc attatgactg gtgacgcctg gacaacagca attcttacat ttttattttc
    17761 cttgctcata aaaaacctcc taaaaaatat ttaaattttt tgcattgaat agattacatg
    17821 agatgttatt tgagaactaa gatacttata ttgtgaataa taatgtaact gtatatttaa
    17881 aataatacat catgatttaa aaaattacaa ttgtacagat ataattgagt ttagatattt
    17941 aaatttaaca acttataata ttttttcaat tagtataaag cctattaatt tgtcttattt
    18001 acaatgaata gtatttactt cttggcaaac aataattaaa aataaaactt ttatatgatt
    18061 aaactctaaa gaaaaagttt ataaagaaga taatataatt aagcagaaaa taatgaaaaa
    18121 aatttattaa agtagacaac gtaaggagaa ttttatgaaa gaaaaaattt attacaatgg
    18181 aaatatcatc acaatggaag attcaatatg tggagatgct attcttataa aagataaaat
    18241 aataaaaaag ataggaacaa aagaagaagt atttgctttg aaaaataaag atacagaaat
    18301 tatagattta caaggtaaga cattgatgcc atcttttata gattcacata gtcacttgat
    18361 agcttttgct actactctaa agctagttcc tttagaagat gcaaccagtt ttaaagacat
    18421 agtaaaaaaa atacaggatt tcaaagagtc gaataatata aaaaaaggag actggataat
    18481 tggattttca tatgataata attttttaga agaaaataag catccagata agagtgtact
    18541 cgatagtgca tcttcagaaa atccaatact aatttcacat gcttctggtc atatgggagc
    18601 tgcaaataca ttggggttaa aacagcttgg tgttaccaat gaaacacgtg acccagaagg
    18661 tggacatata ggtagagtag aaggcagtga agaaccaaat ggatatttag aagaaaatgc
    18721 attttttaat gtagcatcta aaataaaaca accatcatca aatgagattt ttaattcaat
    18781 tgaaaaagca cagaacatat atctaagcta tggtattaca acagctcaag aaggtttaat
    18841 ggaagaaaat caatttaaca ttttaaaagc aatggcaaat caaaataaat taaaaatgga
    18901 tgttgtagga tatgtaaatt tgaaaaaaag taagtctgta ttagataata atagggagtt
    18961 tataaaaaaa tatataaata gatttaaagt aggaggttat aagattttct tagatggttc
    19021 tccacaaggt aagacagcat ggttgagtag accatatgaa aattcagatg atggttattg
    19081 tggatatcct atatacaaag atgaagaagt agaaaagttt atagatattt cactaaaaga
    19141 aaagatgcaa ctcttaactc attgtaatgg agatgcagca gcagaccagt tgatagatgc
    19201 gtttgagaaa gttttaaact taaaagagca aagtagtgaa aataatataa gaccagttat
    19261 gatacatgct caaacagtaa gagcagacca gattgatgat atgaaagtaa taaatatgat
    19321 accttcatac tttgtagctc acacatatta ttggggtgat attcatatca aaaaccttgg
    19381 agaagataga gcgtttaaga ttagcccact taaaacaact atagaaaagg gattaattta
    19441 cactcttcat caggatacac cagtaatagc acctaatatg ctggaaacag tatggtgtgc
    19501 tgtaaataga attacaaaaa aaggtataca aataggagag aatgaaaaga taagcccatt
    19561 agatgcttta aagggtgtta ctataaatgc agcatatcaa tattttgaag aagataaaaa
    19621 aggttcaatt aaagaaggta aattagctaa tttgataata ttagatgaaa acccacttac
    19681 aatcgaccct atgaaaataa aagatattaa agtattgcag actataagag aaggagaagt
    19741 attgtattct ttgaagtaga tgaaagttag atattttata atctataaaa aatagtttta
    19801 tataataagt taggtagaat taaataagat tacagttaaa tcaacaatta tattttttat
    19861 taaattttaa ttattgtatt gaagatttat aagtataatt ttattgattc taccttttta
    19921 aataactgtc gatttattaa agttatctat gtactatatt aaacaattaa gtagttatta
    19981 attgcactta ttagtttatt aaaaaactat gtatattact ttgcagtaag actttggaac
    20041 taaatctata atagcaaatt taaatttatt aaatagatta ccttataaca tttcatataa
    20101 aatatattgt gaatctgaga aaattactat ttttctttga gtatttttta taagcagttc
    20161 taaaagattc aatttcatac ctattacttg aagcattcat taaaataatt ctaatttaaa
    20221 aaataattct atccaaataa tagatatata ggactatgtt ataatataaa aaaatttata
    20281 taaaagttaa taactataca agattgataa agaaaaagtc aaaatagata attgattagc
    20341 aaaatagata taattataat gaagttaaat ctagcaatat tctcctaaaa ttaaatttca
    20401 tttatttaat taaatataat ttgacaatgg acaaaaaagt gagtaaaata taaataaaat
    20461 aaaaataaca ggttcggata aagaaaaagg tttcttaacc tcaagcataa agtatattat
    20521 ctaagttatt tgagattaag agataaatcc aatcttaggt actgaggagg aaaaaatgct
    20581 tgatataaaa agaataagag aaaatttaga ttgtataaaa aaagcaatgg aaagaagggg
    20641 cgaaaaagac tttaaccttg atgaagtagt taagttagat gatgaaagaa gaaagattct
    20701 tcaagaagtt gaagttatga aacataaatt aaatacagca tctaaaaata taccaaatct
    20761 tataaaagaa ggtaaagatg tagaaaatga aaagattaaa ctaaaagagt tatctgataa
    20821 aattaaggta atagaccaaa atcttaaaga agtagaagat aagatggaat acctattaat
    20881 gagaatacca aatgtaccac atccagaagt accacaaggt gaaactgatg aagataatgt
    20941 tgaggtaaga acttggggaa agactactac ctttgatttt gaatctaagg ctcattggga
    21001 aattggaaca gaattaggta tacttgattt tgaaacagcg agtaaaataa ctggttcaag
    21061 attcactctt tacaaaggat tgggtgctag attagagaga gcattattaa atttttactt
    21121 agacacgaat acaaaagtaa atggatatac agaagtcatt ccaccattta tggcaaatag
    21181 aaatagtttt ttaggtacag gacagttacc taagtttgaa gaagatatgt ttaaaataga
    21241 aggtctggac tattttatga ttccaacatc agaagttcct ttaactaata tacatgctaa
    21301 tgaaatactt aaatttgaac aattaccaat aaattataca gcttatactc catgttttag
    21361 gtcagaagct ggttctgctg gaagagatac aagaggttta gtaagacagc atcagtttaa
    21421 caaagtagaa atggttaaaa tagttgcacc agaagaatct tacaatgagt tagaaaagct
    21481 tactaacaat gcggaaacta tgctacaatt attgaatctg ccttatagag tagtaaagat
    21541 atgtacaggt gatttaggat ttacggcttc ttttaaatat gatgtagaag tttggatgcc
    21601 aagttacaat aggtatgttg agatttcatc ttgttcaaac tgcgaagatt tccaagctag
    21661 aagagcagga ataagattta aaagagataa agattctaaa gctgaatatg tacacacatt
    21721 aaatggctct gggttagcta ttggaagaag tgttgcagct atattagaaa attatcaaca
    21781 agaagatggt tctgtggttg ttcctgaagt tttaagacca tatatgggag tttctgttat
    21841 taaataagag ttaaattatg tatttttaag caatacaata atatagaagt aataaaaaat
    21901 aattaatata aaagtgatac aataatatag aagtaataaa aaaatagtta atataaaagt
    21961 gatacaataa taatagaagt aataaaaaaa aataattaat ataaaaatgt gtacaaacta
    22021 atgaatattg atatagtaag ctatcatatg aaagagtatg tacacatttt ttttactaat
    22081 ttttcacaat atgaacataa ataatttaca ggaaaggaat cttttatgaa aattcaaaat
    22141 actgagtggg gatatattga atggaaacat acttatgatg aaaataatcc aaaacaagct
    22201 atgaatatat acattgcagt aactatgcct ggtaagaaac attttaatca tgttcattat
    22261 ggtcaagagc agatgatata tattttagag ggagagggtt tgtatattat aaatggagtt
    22321 tggaagccat tttatcaagg tatgattttt tatatagaat ctggtagcac acatgaaact
    22381 ataaatacag gcgatagaga aataaaagaa cttattgttt caaataatgt agatgatgta
    22441 ggagagagtg aagttataga tattaatcct aataattatt taaagaagac cttgataaat
    22501 tatagtgaaa gtacattaaa tttgtatgca gcagtagaat caattcgtgg tcaatttata
    22561 gacccgttta aaattccatt aattatatat gatgattcat ggaatatagt tttaaaaaac
    22621 ccttattttc cacttttttg ttttgaaaaa tgtaatccta tgaaatttcc tcaaaattgt
    22681 gattgtatga atcaaaaaag tagcaatcaa tttgtatgtg aatatgggat aactatatat
    22741 aatataccaa tactgtacaa aagcaatagc ataggtgtta taagaggagg gtatgtacta
    22801 ttatctgatt taaatttaga tacagagcat aataatttat atgatattcc agagggagct
    22861 gctagaagta taaaacgcct cttgaaacaa atatctaaaa atataataaa tttttgctct
    22921 tttaatgata taagaaagga tttacaagaa aaagagaaga ctatagcaag aacatatcat
    22981 tatggagagc aacttgaaat gaatttaaaa gtagctcaag atatggttac taatttaaga
    23041 ataaatcatc actttttatt taatacatta aattcaatgg caagcatagc actagatgat
    23101 ggtagttatg atttgtatag tgcaataata gatttatcaa gaatgtttag atatacaatg
    23161 aggtctgatt taagatttgt agaattagaa tctgaaatat tatacattaa aaattatttg
    23221 aatttacaga aattacgtta tggagatgca cttaaagtaa aatatcttat tccagagaag
    23281 ttatataacc tttctgtgcc ttttaacttt attcaaccta tcgtagagaa tgcatttaca
    23341 catggattta gagatattga cacagaaaag cgaattgaaa taattgcaag attagattct
    23401 cagtatgcta ttatagagat tcataataat ggaactatct tagatggaaa taatatagac
    23461 aaaataaagg caggtattcg tagtaataat ggtcatggtt tgtctttaat atatacaaaa
    23521 ttcacatctg cttatggaaa taattttgat atggatataa agtcgagtga taatgaagga
    23581 acttacataa tgattaaaat tcctattgaa aattataagg agtatgtata gaacttgtga
    23641 aaaattaata agttttataa atgttaagaa atatattaga tgtttttatg ttgttttata
    23701 tatcttactt ttttatatta ttaagctaaa agggaggtat tctcatgaga agagcactaa
    23761 taatagatga tgaagaagct gttctaaaaa taataacaca ttttatagaa atgaaagata
    23821 tgccaataaa aattgttggt aaagcaacat ctggtgatga agcagtagat aaaatcattg
    23881 gtttagagcc agacatagtt tttatagata ttcaaatgcc aatatacaat gggttagaag
    23941 taatagaaaa aacatctcat ttatctaaaa aaataaactt tgtagtaata actgcattta
    24001 actattttga atatgctcaa aaagctttga gattaaatgt aaaagatata ctccttaaac
    24061 cattggatat gaaagaattt actaagtctg tagaaaaaat tattggatat caatatacca
    24121 ataatgattt attaaatgag atactggaat atataaatct aaactatagc aatgacttaa
    24181 agttaaatga ttgtgctaga ttatttttaa ccaatccatc taatatatct aggattttta
    24241 aaagtaattt aaatacaact tttatatcat atctaaatca tataagaata gaaaaatcta
    24301 ttgaattact tgaaaatact acaaaaagta taaatgagat tgcagaaata gttggatata
    24361 atagcctaaa taacttttat aaaaacttta agatagaaaa aggaatgact ccaaaagttt
    24421 ataaactgaa cactaaaaat tgaaattgat aaataaaaag tcaatataga taattgtttg
    24481 agtgtgtctg aataagtata ctttaattat gataaaaatt aaagcgcttt aaacatattt
    24541 aataatatta aaccttataa aagctgtacc tatttatata aaaaaatata tataaataaa
    24601 aagaaaaaga tatacaaaat aaaaaaagtg aattaaatac tgtttcaaaa agacagaatt
    24661 ttcatttaat tcactgagag tttatatttc ttaaaggaag gtaactttat aatggaggta
    24721 atttaatatg tcagacaaag tgaatatgaa aaatattatc tgtattgcag gcgcttatct
    24781 agcaacagca attggttctg ggtttgctac tggtcaagaa attctacaat tttttgcctc
    24841 acaagggtat atgggtttaa ttggagcatt aatatcaatg gtattgtttt catttcttgg
    24901 tgctgaagtt ataactagag gtagagaatt aaaacttaaa gaaccaacaa gaatttacac
    24961 tttttattgt gggaagattt taggaaaatt ttacgaatgg tttagtccaa tatttttatt
    25021 tgggattctt atagtaatga tttcaggtgc aggctcaact ttaactgaat actatggtat
    25081 aaatccttac ttaggaagag tattgatggc tgttgctact ttaattacag tgtctatggg
    25141 tttagaaaaa ttagtaagca tacttggacg tattggacca gtgatagcag tatttacaat
    25201 tgctgttgga ttaataagct tgtttaagca ttttgatggt ttatctatgg ttggagaagc
    25261 tatgaaaaat attgaagtta caaaagcatc agaaagtcca atcgtttctg gtgtaatata
    25321 taatacatta aatattattg taatgattgt ttttttaaca ggaatgggag cttctattga
    25381 aagtaaaaag gatgcttttt ggggaggttt attaggtgga atagtgttta tgactgctgg
    25441 tatagttatg tatttggcta tgatttcaga tataggaaat ttgtatatga aagaaatacc
    25501 atcactttat ttagcagata atatatctcc tattgtaggt gtatgttttt cagttgcatt
    25561 aatattaggt atctatacaa cagctgttcc acttttatgg tctgtaacca atagaattgt
    25621 tgaagatgaa catcctaaat ttaaattggt aacaacagta atagctattt tggcttgtat
    25681 aggtggtttt ttaccgtttg gtaaattagt aaacatttta tatccatata caggttatat
    25741 ggggctttta attttagtct gtatggtgta taaagcaatt acatgtagaa aaaaatcaac
    25801 acaaagtcag gaatagagtc acctattaat agtttttatt taaagaaatt ttttatataa
    25861 tccgaattta aaaagtcaaa atagttaaat aaattgcaaa acatgataat at
//
LOCUS       DAMKPG010000056        25608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMKPG010000056 DAMKPG010000000
VERSION     DAMKPG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.059"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..954
                     /locus_tag="PC621_002311"
     CDS             <1..954
                     /locus_tag="PC621_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HDF4109460.1"
                     /translation="SMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENK
                     SFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNIHSQAVYRL
                     KKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYN
                     KGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASF
                     GNKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDSSVSIGIA
                     RYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(1644..2474)
                     /locus_tag="PC621_002312"
     CDS             complement(1644..2474)
                     /locus_tag="PC621_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4109461.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            2547..4274
                     /locus_tag="PC621_002313"
     CDS             2547..4274
                     /locus_tag="PC621_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109462.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            4311..6173
                     /locus_tag="PC621_002314"
     CDS             4311..6173
                     /locus_tag="PC621_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109463.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            6506..7195
                     /locus_tag="PC621_002315"
     CDS             6506..7195
                     /locus_tag="PC621_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF4109464.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            7300..7986
                     /locus_tag="PC621_002316"
     CDS             7300..7986
                     /locus_tag="PC621_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF4109465.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            8003..9910
                     /locus_tag="PC621_002317"
     CDS             8003..9910
                     /locus_tag="PC621_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF4109466.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            10363..11052
                     /locus_tag="PC621_002318"
     CDS             10363..11052
                     /locus_tag="PC621_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109467.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            11488..12027
                     /locus_tag="PC621_002319"
     CDS             11488..12027
                     /locus_tag="PC621_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109468.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            12046..13089
                     /locus_tag="PC621_002320"
     CDS             12046..13089
                     /locus_tag="PC621_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109469.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            13091..13927
                     /locus_tag="PC621_002321"
     CDS             13091..13927
                     /locus_tag="PC621_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109470.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            14032..14706
                     /locus_tag="PC621_002322"
     CDS             14032..14706
                     /locus_tag="PC621_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109471.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            14706..15758
                     /locus_tag="PC621_002323"
     CDS             14706..15758
                     /locus_tag="PC621_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4109472.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            16202..17050
                     /locus_tag="PC621_002324"
     CDS             16202..17050
                     /locus_tag="PC621_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4109473.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            17040..18704
                     /locus_tag="PC621_002325"
     CDS             17040..18704
                     /locus_tag="PC621_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4109474.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            18753..20009
                     /locus_tag="PC621_002326"
     CDS             18753..20009
                     /locus_tag="PC621_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4109475.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            20032..22113
                     /locus_tag="PC621_002327"
     CDS             20032..22113
                     /locus_tag="PC621_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF4109476.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            22150..23235
                     /locus_tag="PC621_002328"
     CDS             22150..23235
                     /locus_tag="PC621_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF4109477.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDGSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWVENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDSSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            23289..24413
                     /gene="wecB"
                     /locus_tag="PC621_002329"
     CDS             23289..24413
                     /gene="wecB"
                     /locus_tag="PC621_002329"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF4109478.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            24491..25465
                     /locus_tag="PC621_002330"
     CDS             24491..25465
                     /locus_tag="PC621_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF4109479.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT        10194 a         2551 c         4394 g         8469 t
ORIGIN      
        1 tctatgaaat atgaaatact tgaaaaagaa ataattcata aggaagaatt aagacttagt
       61 aatgagtgtt ataaaattat tgttgaacag actgattctg tcgttttcga atggaatatc
      121 aaagaaaata aatcatttgt atcgcaggct tggactgaaa aatttggata taacaatgca
      181 tgtaagaata tttttaaaga aattaaagat aaagacttag ttcattctga agataaagct
      241 atatttgaag gttttcttga aagtattaaa aaaaagaata tacacagtca agcagtatat
      301 cgtttaaaaa aatctaatgg tgaatatatt tggtgtagaa catcaattac aagcatttat
      361 aatgatgaaa atgaactttt aagagttgta ggagtaattg tagacgtaga tagtgatata
      421 aaaaaatatg aagagttaag aactagagca gaaagtgact cattgaccaa tatatacaat
      481 aaaggtacat ttgaaaaatt agtggaagaa actatagtta tgaatacagg tgataaaaaa
      541 gatgcattat tcattattga tttagatgat tttaaagaaa taaatgataa ttttggtcat
      601 ccatttggtg attttgtact aaaaacattt gctgataaga tacaagctag ttttggtaat
      661 aaagacttag tcggaagaat tggtggagat gaatttgttg tatatatgca agattatgtt
      721 acagaagtaa atttgcataa aaaagctaaa gaattaaata gagtactttc ggataattac
      781 actgatttga gttttagttt tgattcttct gtcagtatag gtattgcaag gtatcctcaa
      841 gatggaactt cattttttga attgtttaaa aatgcagata gagcacttta tagcataaaa
      901 gcatcaggta aaaattcata ttgtttattt gaagaagaac tatatgtaca gtgaatttga
      961 atttagattt tatgtgagag taagatttat atatacttgt ggctgtctga tttataaaat
     1021 atgttacttt gtatattttt atatgtttca gtaaggttaa ttaacataag caaaggtgta
     1081 agtaaattta tattagttta agtttactta cacctttata aaaataaatt ttgtaaattt
     1141 aaacagttgg ttttataagg atttttattt attttaatat cttagatatt cctttttgta
     1201 ttccttagag aaaataaata atgaaaggag tacagaaaaa attaaacatc ctaatatacc
     1261 acttgaatta agtcttaaag cattaaagtt taaaaaagca ttatttaaat cggtatccaa
     1321 aagaaaagga tgctttgatt tgctatatat ggaaaaatta aaaatatatt cataatcaaa
     1381 tttataaaat taatatcttt ccatcctaac attacattta cttagattaa ttattattat
     1441 aaaaataaaa atacaaataa tagattcatt tacattctta aatgattaaa gctctttacc
     1501 ctttaaacat gcataacata tcgttataat tccagtaaat aagactaaaa tgcccattcc
     1561 taaaattgca gtttcatcat ctattactcc aaatgctttt aaagtaactt atttcgcttt
     1621 gtataataat aatttgtctc tttttatatt ttaacttctg ctgagatttg agttagatat
     1681 tcgttagaat ctcgcacact tatatcatat agaagatatt cttcataaac aaaatcccca
     1741 atacgtaaat tattctgatt aaaaaaattt aataactttt cataagcctt gtaaatagtt
     1801 tcataactac ctttatgata agcagttaca taaagtccct ttggtttgat tgatacagga
     1861 tatttcttgt actctttatt aacttttgta aataaatatg aatatgattc aaagtcttta
     1921 ttaattatat cttcaccttt aatgattgag ccaatcgtac catagtcact caattcatag
     1981 ttgtccataa agttaataca ttcatataaa aaattttctt cctcatctcc taaaaactgt
     2041 tctttatatg taataggtgt tttgaccaag tactcttcct catgttcttt taaagtaata
     2101 gtattcgcat ctatattaca ggcattttta gtgaatgaaa tagtagattc cattagagct
     2161 tgaatattat ttagtttttc aatttcattt ttaatatcaa tggttttctt ttctaaaagc
     2221 tcaattagta aattaggtgt tcttttgtct atataagctt taatttcttt taaaggcatt
     2281 tttacttccc taagtgaaga aattatacca aaaatgctaa gttgatggta tgagtagtat
     2341 ctatatccat tttctttctt taattctggt gagaaaagac ctatatcatc ataatggaaa
     2401 agtgtttttt tattgattcc acaaatcttt gcaaattctc cagttgtaaa ataatttttt
     2461 gaaacttgac tcataattat aattcctcct tgactatcct gttacagtat agtttatcat
     2521 agtattttgt aaaggagtgt gattatatga aaaaattaat tcatttttta aaaccatata
     2581 gagttttaat tgttgtagtt ttgatattta cgtttttaca aacacttgga actttgtata
     2641 taccaacact tacagcaaat atagtaaaca atggtgtagt aaagggagat atagattata
     2701 ttgtaaaaac aggtttaata atgatgatag tagctggtat aacagcttta tcagctgtgt
     2761 tagtttgtaa agtatcagca aatctttcat ctggattttg tagagatatt agagaagcag
     2821 tatttgtaaa atcacaagat ttatcaataa atgattttaa taatataggt acagcatcaa
     2881 tgattactag gagtactagt gatattactt taataggtca gtctgtgttt atgtttattc
     2941 aattggtatt accagcacca attataactg tttctggtct attcttagca tattctatag
     3001 ataaagcaat gacaattatt attgttgtag taatgttttt attcatgctt tcagcatttt
     3061 tagttggtaa aaagcttata aagttgttta aaatgatgca aataaagatg gacaatatga
     3121 atagggtatt acgtgaggta gtaactggag taagagttat aagagctttt aatagggagc
     3181 attttgaaaa aaagagattt gatagaactg caatagatta ttctgaaaca gcaatttcaa
     3241 taaataaagt atttgcagta cttatgccta ttgtaatgtt aataatgaat ttaggtatag
     3301 taagtattat ttggtttggt gggatgagag tcagtaatgg aaatatggag ataggtcata
     3361 taatggcact tattgaatat tgtatcttaa ttttattcta tttaatcatg ggagtaatgg
     3421 tatttatgta catacctaga gcaggtgcat gtgcagatag agtaaatcag atattagata
     3481 tagaacctga aattgtagat ggaaatggtc gtaaagatac tgtctcagag aggggacatt
     3541 tagtatttaa aaatgtaaca ttttcatatg cacaatcaga agaacctgtg ttaaataata
     3601 taacatttga agcaaaatct ggagaagtaa ctgcaattat aggaagcact ggctcaggca
     3661 aatctactat tgcaaatata ataccacgtt tttttgaaat tcaatctgga gaaatatcta
     3721 taaatggtca ggatgttaag aagattcctc aaaaagaact tagagataag attggatttg
     3781 tgccacaaaa agctttttta tttagtggaa caatagaaga aaatataagg tatggaaaag
     3841 aagatgcaag tattgaagaa ataaagcatg ctgcaagtat agctcaagct gatgaattta
     3901 tttctgatat ggaagacaag tatgactcat ttgtagcaca aggtggaaat aatttgtcag
     3961 gtggtcaaaa acagagaata tccattgcaa gagcgttagt tagaaagcct gaagtatatg
     4021 tctttgatga tagtttttct gcattagatt ttaaaacaga taaaagactt agaaaagcgt
     4081 tgaaaaatga aatcaaagat tcatcagcta taattatagc acaacgtata agtacaatca
     4141 tggatgctaa tcagattata gttttaaatg atggaaaaat tgtaggtatt ggaaaacata
     4201 aagatttact tgaaaattgt gaagtttata agcaaattgc ggattcacaa cttagtaaag
     4261 aagaacttgc ataaaatgaa gaattaatct gatatttgga ggtgtatgat atgagtgaaa
     4321 aaagaataga tgaacaagat atagaagtac ttagttttga agatggcatg tcagcagaaa
     4381 aagttaagga tgcaaaaaaa actactaaga gattattaaa atatatggca aaacaaaagt
     4441 ttaaaatatt aattattttt atttcagtat tgataagttc agcgcttaca gttttagcac
     4501 ctatggttat gggaaaagct attgaccaac tatttaatgg aataaaaact gcagtccaaa
     4561 caggtacaaa attcagtgtg aattttagta ctatgggggg gatagtatca atacttctag
     4621 ggttgtactt aattagtgca gtatttatat atattcaaca gtatattatg tcaggagttg
     4681 cacaaaatct agttcttagc atgagaaaag atttaagtga taaactcaat aaacttcctc
     4741 ttaagtacta tgattctcat aaaaaggggg aaacacttag tatagtaaca aatgatttag
     4801 aaaaagtagc agatagttta caagaaggat taatgcaact tattacagca gtagttacag
     4861 tagttggttc tattgtaatg atgataagta taagtgtacc acttacaata gtatcagcta
     4921 taacattact tgtttcattg ggtattactg ttgtaattgc aagaaaatca caagaaagat
     4981 tctctgaaaa tcaaaaggca ctaggagagt taaatagcaa tatagaagag atatttactg
     5041 gtcaaatagt ggttaaagca tttagtaaag aaaaggatac tataagaaac tttaaaatat
     5101 taaatcaaaa tctttataat gcaagtagaa gagcacagtt ttcgtcatat gcaatatctc
     5161 caattattag atttataaac ctaattggct atgttattat agcagttgta ggtgggatat
     5221 ttgcatcaac tggagcaatg actcttggta gtatacaagc gtttattcaa tatgtaaatc
     5281 aagcatcaga gcctactaca gaaatatctt atattgtaaa tatgcttcaa gcagcaatag
     5341 catctgcaga gagagttttt acagttatgg atgaggtgga agagataaag gatgaagaat
     5401 cttcaaaggt tattagcatg cctaaaggta aagtacaatt tgagcatgtt aaatttggat
     5461 atagtgatga ttttattctt atgaaagata ttaatatcaa tctaaatgca ggagacaaga
     5521 ttgcaattgt tggacctaca ggagctggaa agacaactct tgtaaattta cttatgagat
     5581 tttatgagat acaaggtgga agaattacta ttgatggtgt taatataaaa gacttaaagc
     5641 gtggagaact tagaactatg tttggtatgg ttcttcaaga tacatggctg tttaatggaa
     5701 gtataaaaga aaatattgca tatagtaagt cagatgccac aatggatgaa attgtaagag
     5761 cagcaaaatc agcaagagta gaccatttta taaggacttt accacaaggg tatgacacta
     5821 tattgactga ggatgcttct aatctatctc aaggtcaaaa gcaacttcta acaattgcca
     5881 gggcaatact ttcagaccca tcagtattga ttcttgatga agcaacatct agtgtagata
     5941 ctagaacaga ggtagaaatt caaaaggcaa tgaataactt gatgaaggga agaactagtt
     6001 ttgttattgc acatagatta tcaactattc gagatgctga tttaatatta gtaatgaaag
     6061 aaggcactat tattgaacaa ggaagccata aggaacttat tgaaaagaaa ggattctatg
     6121 aagaacttta taatagtcaa ttttcaagtg aatatgatga agaagttgta tgattatcaa
     6181 gttttaggca actaaatgat tgataaatat taagttaaat atagtaacat gtaaggttat
     6241 atggaataag agtatttctc tatttcatat aacctttttt atttatcaaa ataagataaa
     6301 attttctttc taaattagat tgaagaaagt atttatcaat ataaaagtaa aataatcatt
     6361 attattagtg aagtaaatat tgtttgccac atatatgcca taaagatgca cttattttac
     6421 ggagatttaa caaagtagtg taaagtatac tctaaatata aaataaatca aatagagtta
     6481 aataaattga gaggtgatat aaatagtgaa taaaaaattg aactatagat ttgaaatgaa
     6541 gcacaaaata actgaagcag atgtgttagc tttaaaatca agactctatc caataatgaa
     6601 gaaagatgaa aatgcatcta aagatggaaa atatcttatt aggagtttat attttgatac
     6661 tcctgaagat aaagcacttt tagataaatt aaatggcgtt gcaataagag aaaagtttag
     6721 gataagattt tataataata actgctctta tataaggtta gagaaaaaaa tcaaacatta
     6781 caacatgaca tcaaagctat caactaattt aaccaaaaaa gaagtaataa atatactaaa
     6841 caatgatatt gaatttctaa aagagtcgac aaattgttta ttaagagaat tttatttgaa
     6901 attaaaatgt gaaagattag aggcaaaaag catagttgat tacaatagag aagcttttat
     6961 ttatccagtt ggaaatgtaa gagttacaat cgatagtgat attaaaactt ctgtaaattc
     7021 gatagattta tttaataaag atttgccgac tgtttcggta attgatgaga atatgactgt
     7081 attggaggtt aaatatgatg aatttatacc agattttata aaggatttaa ttcaaattaa
     7141 taagactact tctacagcag tttcaaagta tgcttcaagt agattatata tttaagaata
     7201 aaggctatga tttatataaa gatatacata aataaggtta aaaaacaaat aggttaaaaa
     7261 taaataaaat aaaaaataaa taagttaaaa ataggaagga tgattttaat tatgacattt
     7321 aatgatattt ttaaatcaag ttttatagaa aacgtatcag gtttttcatt tgtggacagt
     7381 gctttagcac taggttcagc ttttttagta ggtttgttta tatatatggt ctataaaaag
     7441 acttatatgg gaattatgta ttctcgacca ttcaatgtgt cgcttgtagc acttacaatg
     7501 cttacaactt ttgtaatatt ggcggttact tcaaatgtag ttttatcctt aggtatggtt
     7561 ggtgctttat ctatagtaag atttagaaca gcaattaaag acccaatgga tttagtattt
     7621 ctattttggt cattgggttc aggtatagtt ttaggagcag gtttgattcc cttagctgtc
     7681 atgggttcaa taattatggg attgattcta atattctttt caaataaaac aatatctgaa
     7741 actccatata tattgatggt aaattgtaat aatgaagatt cagaagatat agcaactgat
     7801 aaaattaaaa aagtatttac taaatatcaa ataaaatcaa aaagtgttac accagaaaaa
     7861 ggaatagaat tagtgtttga agtaagaatg aaatatggag agacaagtct tataaatgac
     7921 ttgagtcaag tagatggagt tactaatgca gttcttgtaa gctataatgg tgactatgta
     7981 gcatagagag gagtaatctt ttatgaaaga taaaaaattt acccttctta tctctattat
     8041 gattatattt ttatgtgctg tagttggagt ttatagtaca tctagcaaca aaagtgttga
     8101 tttatatagt gatgtatata ttgaaaaata ttttaacaga gacaaggtta tggaagttaa
     8161 tatagagata gatgaaagtg acttgaagga tatgaatgaa aatgctataa aagaagaatt
     8221 taaggttgca aaagtaactg tagatggaga tacatatgga aacgtaggta taagaactaa
     8281 aggaaattca agtcttacat ctgtagcaaa tagtgatagt gatagataca gctataagat
     8341 taattttgat aagtataata ctagtcaaag tatggaaggg cttactcaat taaatcttaa
     8401 taactgttac tctgacccat cttatatgag agagttttta acatatagta tttgcgagga
     8461 aatgggatta gcgactccag aatttgcata tgctaaagtc tctataaatg gcgaatatca
     8521 tggtttgtat ttggcagtag aaggattaaa agagtcttat cttgaaaata attttggtaa
     8581 tgtaactgga gacttatata agtcagatga aggaagctcg ttgcaatata aaggagatga
     8641 cccagaaagt tactcaaact taatcgttga aagtgataaa aagacagctg attggtctaa
     8701 aattacaaaa ctattaaaat ctttggatac aggtgaagat attgaaaaat atcttgatgt
     8761 agattctgtc cttaaaaata tagcaataaa tacagcttta ttaaaccttg atagctatca
     8821 aggcagtttt gcccataact attatttata tgagcaagat ggagtatttt ctatgttacc
     8881 atgggatttt aatatgtcat ttggtggatt tagtggtttt ggtggaggta gtcaatctat
     8941 agcaattgat gaacctacga caggtaattt agaagacaga cctctcatat cctcgttatt
     9001 aaaaaatgag acacacaaaa caaaatacca taaatatctg gaagagatag taacaaaata
     9061 cctagattca gactatttag agaatatgac aacaaaattg catgacatga tagcatcata
     9121 tgtaaaagaa gacccaacag cattttatac ttatgaagaa tttgaaaaaa atataacatc
     9181 ttcaattgaa gattctagtg ataataaggg atttggtaat aaagggtttg acaacaataa
     9241 ctctaataac agtgattcta ataataattc taatagtgaa aataagcgct ctggaaatca
     9301 aagtgataaa aaagaagtta atgctgaatt aacatcaagc gtagtcaaaa ctaatacaga
     9361 taatgaaact gaaaataaaa ctacaaatga tagcgaaagt aagaataata cagataaaga
     9421 taaaagtgga aatgataata atcaaaagct agaaggtcct aggggtaaag gaggtaagtc
     9481 aataccaggg gttttggaag ttgcagaaga tatgagtaaa actataaaat ctcaattaag
     9541 tggagaaact tcttcgacaa agcaaaactc tggtgatgaa agttcaagtg gaattaaagg
     9601 tagtgaaaag tttgatgagg atatgagtgg tatgccagaa ccacctgagg gaatggatgg
     9661 taaaatgcca ccaggaatgg gtaatatgga taagggagat atgaatggta aaaatggcaa
     9721 tatgaatatg gatagaaatc aagataatcc aagagaagct ggaggttttg gcaatagagg
     9781 aggaggctct gtgagtaaaa caacaacata cttcaaatta attttaggtg gagcttcaat
     9841 gataataatg tcgattatgt tagtaggtgt atcaagggta aagagaagaa gatttataaa
     9901 gtcaaaataa gattaaaatt gaatataaat aattttaata ctgttaagtc taaatattta
     9961 cttagcagta tttttttaga aaataatgtg tttgattaat aatataaggt aataaattga
    10021 taaaaattaa atacagcata ttaataatac ataataaagt aataaaaaaa ctgtttaagt
    10081 ataataactt atatttagac agtttttttc atttataaat aatataaatg aaattgaaaa
    10141 catttgaagg atagaaattg gttggataaa tgtataaatt acttcaccaa aaaataatga
    10201 caagagttgt aactaagttg taataaaatg gtagttattt agcataaaat aatgtatcat
    10261 agaagtatat tataaaatta ataaaataag atttttgtaa gaaaactgct ggcttttgat
    10321 atttagagtg agtagttaaa ttatgtttag gaggggtaga atttgttgca aaaagtatta
    10381 tggaaatttt taaagctagt agtcggttta tttatatgtt cagttggtat agtattgacg
    10441 ataaattgta accttggatt atctccctgg gatgtatttc atcaaggact ttcaaatcat
    10501 ataggaataa ctataggaac tgcttctata attgtaggct ctatagtagt tattgctgat
    10561 gtcgttcttg gagaaaatgt aggatgggga actgttttta acatgctact aattggattc
    10621 tttatggatt tacttttata tagtaattta attccagagg ctgatagttt atttgtagga
    10681 ataattatgt tgatattagg attggttctt ttgtctgttg gaatggtatt ttatatggga
    10741 agtggtttag gaagtggacc tagagatggt ttgatggttg caattcaaaa gaaaacagga
    10801 aaatcactta aattaataag aggtactata gaagttggag cattaatagt aggattttta
    10861 ttaggtggaa aagttggtat aggaactata ataagtgcat ttggattggg gtatttcaca
    10921 caaatggtat ttggcttatt taaattagat tgtagtaaga ttaaacatag atttatagta
    10981 gatgatataa aatttatcaa agtgtatatg agtggtgaga aaaaagctgc tgttcaaaat
    11041 atagttaagt aatttaagaa taaattcaac gaaatgttaa gtattttaaa ttaacatttt
    11101 atttagatgg ataaattgaa tattaaaaga atattaaaat atgaggattg ctagaacact
    11161 ataaaatata gatttctagc aattttttat ttgttcaatg tttttttagt actagaagtc
    11221 ttattctcta tttaatgaat gaattaattt ataattagag atactaaact ttatagatat
    11281 taatataaac acctttaaac tgtgtatttt ccaaagcctt taaaatgctt gtatttattc
    11341 taaataaaaa aataaaaaaa tgtcaaaaaa actcttgtat tatatccaaa ttttatatat
    11401 aattacctat gttgtttaga aattttaaac tcaatgttta gtaaacaaaa tgtttagtaa
    11461 aataaaacag aaactaggag gtgcaacatg gatataggtg aaaagataaa aagattaaga
    11521 acagaaaaac aactgaccca ggaagagcta gctaatagat gtgaattgtc aaagggattt
    11581 atttctcaat tagaaaataa cttaacatca ccatccatag ctacccttat agatatactt
    11641 gaaatactag gtacaaattt aagggaattt tttaatgaaa tagatgatga aagaatcagt
    11701 tttacaaaag aagatatgtt tgaaactgaa gatgaagatt taaaatataa attaaagtgg
    11761 ttaattccaa attcacaaaa aaatgaaatg gagccaataa taataacatt gtatccagga
    11821 ggacagtata aagaagaaaa gccacatgaa ggtgaagaat ttggatatgt attagcaggt
    11881 tcaatatatg tccatatagg cgaaaagaaa aataaagtaa aaaaaggaga gagtttttac
    11941 tttagaccaa aagcaaatca ctatatatca aacgaaggca aaactacagc aaaagtgatt
    12001 tgggtaagta cgccaccatc attttagaat agaggtgttt aataattggt agaaaatata
    12061 atagaattaa gggatatatc taaacattat gaggaactta ctgttttaga taatttcaat
    12121 ttagatataa agaaaaatga gtttttaaca ttacttggac caagtggatg tggaaaaact
    12181 acaacattaa aaataatagc aggatttgag tatgctgatg atggtaaagt tttatttgaa
    12241 ggtaaggaga taaataactt gccaccatat gaaagacaag taaacacagt atttcaaaaa
    12301 tatgctttat ttccacacat ggatgtttat gaaaatgtag cttttggatt aaaaattaaa
    12361 aagactccaa aagatgtaat agatgctaaa gtaaaagagg ttttgaaact tgttgcacta
    12421 gaaggttttg aaagacgaca agttgaatca ttgagtggag gacaacaaca gagaattgca
    12481 atagcaagag ctctagtcaa tgaaccaaag gtattattac tagatgaacc acttggagca
    12541 cttgatttaa agttaagaca agagatgcaa atagaattaa agagaatcca aaaaaaatta
    12601 ggaataacat ttgtttttgt aactcatgac caagaagagg ctcttactat gtcagatact
    12661 attgttgtaa tgtataaggg caaaattcaa caaatgggaa caccacaaga tatatacaat
    12721 gaacctaaaa attcatttgt agctaaattt ataggtgaaa gtaacatatt tgatggaatt
    12781 atgatagaag attataaagt taatttctgt aatagagatt ttgagtgtgt tgataaagga
    12841 ttcgaaaaaa atgaaaatat agaagtagta ataagaccag aagatataaa aatggtaaag
    12901 ccagaagaag gaatgttaaa ggggaaagtt acatctacag tgtttaaagg agtgcattac
    12961 gagatagaat tgaatgaaaa tggaagattg tggatacttc ataataccaa aaatgctgag
    13021 gttgggactg aactaggcat ggatatatat ccagaagata tccatataat gagaaaagag
    13081 aataactaag atgaaaagaa aatctttttt agcatatcca tatgtagttt ggagtgcgat
    13141 atttgtaata attcctttaa tattagtggt attttttagt tttacaaaag aatcaggtgg
    13201 aggttatgcc tttacacttg agaattataa agaagtaatt gaccctatat atatgaaagt
    13261 ttttgggcgt tctattcttt tagcaggagg agctacatta atatgcctta tagttggtta
    13321 tccagtggca tatataattt caaaggctcg tgtaagtaga agaggttcat taatattatt
    13381 gtttattctt cctatgtgga tgaactttct tttaagaaca tatgcatggg ttgctatatt
    13441 aggaaaaaat ggacttttaa atacattttt aggttggttt ggaatacaac cattagcaat
    13501 tttgtatact aattttgcta tacttttagg tatggtgtat aactttttac catttatggt
    13561 tcttccaata tatacagcac tatcaaagat ggataatgat ttgataaacg ctgctcatga
    13621 cttgggtgca aataatatga cagtttttag aaaaattata tttcctttga gtttgccagg
    13681 tgtaatgtct ggaataacta tggtatttat gccagcagtc actacttttg caatatcaag
    13741 actgttaggt ggaggtaaaa taatgctggt tggagatttg attgaacaac agtttacagt
    13801 tgttggagat tggaactttg gttcagctat atcgatattt atgatgatag taatattaat
    13861 atctatgtct ataatgtcaa aatttggaga tgaatctgac aaagaaggcg gtgggttact
    13921 attttaaaat taaagaaaat aacttctaat atatatttag cattagtatt cttatttttg
    13981 tatgcaccca tatttgcttt ggtactattt tctttcaatg attcaaaatc aatggcaaga
    14041 tggaatggat ttacatggaa gtggtatggt caattacttc aaaatgagag tataatgagt
    14101 gctttatatt atactattgt aatagctata ttagcgtctg taatatctac aatagtagga
    14161 actataagtg ctataggaat acataagatg agaggtaaga gtaagaaatt aatactaaat
    14221 gtaaactatc ttcctatttt aaatacagag atagttacag cagtagctct tatgagcctg
    14281 tttgtatttg taaaaatgga atttggattt acaacaatgc tattggcaca tataatgttt
    14341 tgtttgccat acgttatact ttcagttttg ccaaaaatga agcaattacc tgataatata
    14401 gaagatgcag ctatggactt gggagcaaca cctatatacg ctcttagaaa agtaatttta
    14461 ccacaaataa aaccaggtat agtttctgga tttttaattg catttactat gtcaatagat
    14521 gactttataa taagtttctt taatgctgga aatggtgtaa gcaatctttc tatagaaatc
    14581 tatggaatgg ctagaagagg tataaaacct gaaataaatg cattatcaac aataatgttt
    14641 gcagtggttc taggtttact tttactagct aataaaaaag aatctatagt aagggggatt
    14701 aaataatgat taagtttaaa aaaacaatat cccttatagc tgtaggtatg atgacagtta
    14761 ctttatttac aggatgtaat aaagcaactg attctacaaa agttttaaat gtatataatg
    14821 ttggagatta tatagatgag tctcttatag ataagtttga aaaagaaaca ggaatagatg
    14881 ttcaatattc aacttatgat actaatgaaa tgatgtatca aaaagtaaag agtggtagta
    14941 cacactatga tttagtattt ccttcagact atatggtaga gaaaatgaaa aatgaaggtc
    15001 ttttggaaaa attagatttt aagaatatac ctaatatgaa atatttagat aagagttttt
    15061 taaatcctgt atacgacaaa actaatgaat atagtgttgc atatatgtgg ggaactcttg
    15121 gaatattgta caataagaaa gaagttaaag accctatgga tagctggaat atactttgga
    15181 atccaaagta taagggaaat ataatgatgt ttgactctgt tagagatact atagggataa
    15241 ctcttaaaaa attaggatat agcatgaata gtgtaaatcc aaaagaaata aatgaagcta
    15301 aaaatctttt aatgaaacaa aaggatttag tattggctta tgtaaatgat gaaggtaagg
    15361 atagattgct tggtggagaa gtggcaatgg gtatgctgta ttctggagat gcagttacgc
    15421 taatggagca aaatcctgac cttgattatg ctatacctaa agagggaact aataaatggg
    15481 tagatgctat gtgtgtacct acaacagcac aaaataagaa agaagctgag ctgtttataa
    15541 actttttatt agacccagag aatgcaaaag taaatgctga gtacattggg tattcaacac
    15601 ctaatacagg agcactaaaa ttattagacc cagaaataac tcagaatcct gttgcatatc
    15661 catctaaaga agtacttgat aaatgtgaaa cttttgaaga tattggtgaa aatataaaat
    15721 tatatgacaa ggcttggata gagttaaaat caaaataggg tcttgttggt ttaagtaaat
    15781 ttacttaata aatacttaat aaaactgttc ttttttagaa aatttatttt ctaattattg
    15841 aacagtttta ttttttgttt aggtcatgtt tttattttga gtttattttt atgagaattt
    15901 aaagtacagt aagtttcatt gattactatt tttataataa taactataaa gaggttgtct
    15961 cgaaattgag ataagcctct ttttcattgt tgaaatgatg aaaacatgtt tgatttcata
    16021 aaaaaagagt gattcatgag ctaaagaatt tgctttgaga cagtattttt tattaagtta
    16081 tattcatatg caatttaaat agtttattaa taaatgaaaa cttatttgta taatgatttt
    16141 aataataatt agaaaaaatg ggcaacttaa tagaataatt ggaggtgtaa atttataaaa
    16201 aatgaatagg ataaataaaa aaatagattt attaatattg gctttgctcg tttttatatt
    16261 tttaattgtt ggaatagtaa tactatcgat taggacagaa aataaattga atgtgttcat
    16321 aatgttagca ataatttttt ttataataat gttaacttat ttatcaaatt cagtagtggg
    16381 gttgataaca agttcaataa ttatatttat gtatacatcc tatattttat ataacaatat
    16441 tactcacaat atggatgttg aatttatatc atatatgtgg ataatagcaa caccagtatc
    16501 ttcaattata atgggtaatc tgaacaaaag cataaatgaa ttgcaaaata caaataaaaa
    16561 actatctgaa cagtataagg aattagtgac tattgatagt gaaactggac ttagaaattt
    16621 aaagattttt tataatgatg taaatatgga gataagtaaa tctattagac ataatactga
    16681 tttttctctt atgatagtaa aattaccata ctacggaaac ctacagacta tatttggtga
    16741 aaacaaaact aataaaatag taaaatatat tggttcaaat attatagaat gtacaagaaa
    16801 tgaagatata atttatagtt tacaaaaaga tatgataggg atactaatgc caaatacatc
    16861 tttggaaggt tcgaaagttg ttaaagacag aataaagaaa aggataaaag aattaaattt
    16921 agatttaaac aataggggaa aatatgtaaa tatagatgtc aaaattgcat ttttacaata
    16981 taagagtagt tttggagata gtataaattt taagaatata gttgaagagg aacttcaata
    17041 tgatgtttaa aatatttaat aaaagtttat taaaattagt aattgtatta tcaatagtac
    17101 tgacaatatc aaattttagc ttattttcat atgcatcaga aattacttcc agcacagata
    17161 aggtattaat catatatgat tctaagaaag aaacagctta taatagagat attttaaata
    17221 ttatgagaac tctattggga agatttagtt cagatataga gctattaaaa ttgagtaact
    17281 atgatggtga gataaatgaa aattattaca gtcatatatt tattttagga atcaatgaaa
    17341 actcatataa taatgataaa aacactaaaa acttgattag ttccttaaat tcatataaag
    17401 taactatatg ttggttagga tatggtatag aaaatctttt ggaacataaa aaatacaatt
    17461 tagactatgt aggaaaaaca aacaatatag ttagtgtaaa ttatagaggt aaatcatata
    17521 acttggatga acattatgtt tttaacattg tagaatcaaa agatacaagt aataaagtaa
    17581 taggaagtat taatgatacc ttaaataagt atccatatat aataaatgat aaaaatcttt
    17641 tttatgtatc aaaattagat ttagatggag ttttatttta tatattttgc gattcactta
    17701 atgatatttt taatattaag acgtttgata aaggacgtat atttgtaaga atagaagatg
    17761 tacatgcttt tagagaacca aaaaatcttg tagagatagc ggattattta agtagtaaga
    17821 atataccatt tacgattgca ttaataccag catatgtaaa tcctaaaaat cataaagtaa
    17881 taactttatc agaaagccct gaaattgtca aagcaataaa atatatgcaa gataaaggtg
    17941 gaactgtgat attgcatgga tatactcatc aatacaagaa agaagaagtt tctggagaag
    18001 ggtatgaatt ttgggatggt aaaaaagatg aacccttaaa agaaaatatg aagatatttg
    18061 tcaaagatag agttttgaat ggtcttagag tatgtataga gaatggaatt tatcctcttg
    18121 catttgaagc gccacattat gcaatggaat cagagggata taaagaatta aaaaaatatt
    18181 tttctactta tatggggcaa catcaaaata atgataaaaa atttagcaca aatacatatc
    18241 cttatattat tagggataca gaggagttta atatatttat acccgaaaat ttaggatata
    18301 tagacccaga agacaagttt acttttcaaa atataaaaga aaatttagat aaattatcta
    18361 ttgtaagagg ttttagtgga gggttcttct ttcattcata tttaaacata gagtatctaa
    18421 aaaacacaat agaatattta gaaaaacaga atatagaatt tatgaattta agagatttta
    18481 ataattgggt aaaagttgat gaaatacaaa taagaaataa tggagatgaa attatagtca
    18541 actatgataa ggacttagat gaaataacta aaagtgatac tagatttaaa tacatatcca
    18601 atattagtaa aattttaata ttcatagtat ctataagtgt tttaatattt gtgattatat
    18661 ttatatattt taagagaata gataaaaaga aatttttaaa ataaatgatt ttatattaga
    18721 atcaatattt taaaatattt taggagaaat ttatgagaga tttaaatgta tcagagtatt
    18781 tgttcgtatt tagtctgttt agtatatggt ctttattgct tataaatatt attttagcta
    18841 tgggtggata tatattttat tttaaaaact ttgataaaga aataaaagaa attgatgaat
    18901 atcctatgat atcaatactt gttcctgctc ataatgaagc taaagtgata ggtagaactg
    18961 tggagtcttt gttattactc aattatccta agagtaagat ggaacttatt gttataaatg
    19021 acaattcttc tgataatagt aaagaaattt tagaaaatat aaaggatagg tacaacaatt
    19081 ataatttcac tataataaat acagatagtt taacaggtgg aaaaggaaag tctaatgcat
    19141 taaatattgg atataaggtt tcaaaaggag attttatagc tgtttatgat gcagataaca
    19201 caccagataa aaatgcactt agatatctag tacaaactat agtaatgaat gatgaattag
    19261 gtgctgttat tgggaaattt aggactcgaa ataaaaataa aaacctatta accaaattta
    19321 taaacataga aacattaagc tttcaatgga tgtcacaagc tggtagatgg caacttttta
    19381 atctatgtac aataccagga actaacttta tattgaggag aagtatcata gaagaaattg
    19441 aaggatggga tagtaaagca attgcagaag atacagaaat aagttttagg atatataaat
    19501 tgggatacaa gataaagctt gtaccacaat ctataacatg ggaacaagag ccagaaactg
    19561 ttaaggtgtg gataaagcaa cgtactagat gggcaaaagg gaatatatat gtattaatga
    19621 aatatataaa aaatatattt aaacagggaa gaaataagat tgtgtttgat atagcttact
    19681 ttttttctgt atattttcta tttttaacaa ctgtaattat atccgatatt ttatgtgttt
    19741 taagtatatc taaactagta gaaatttcta ttccaattaa cttttttcta atatggatac
    19801 tatcttatct tttatttatt atagaagtga gtataagcct tactatagaa aaaggtgaag
    19861 caacaattga aaatatattc atagttgcaa taatgtattt tacatactct caactatggc
    19921 tgtttgtagc tataaaaggg atgatagagt atctaaaaga tataattttt aaaagagagg
    19981 ttaaatggta taaaacagaa agattttgat tcatgtacag gaggaattaa catgaaaaaa
    20041 tttattatat caattatatc tttagtattg tttttttcaa atatatcact tatatacaaa
    20101 gtaaatgctg atgagactaa agttaaaaat tataagtttg aaagagatat aactattgat
    20161 ggtgttatag gaagtaatag tacttttttt gaagtaaata aaaattggga tattgaagaa
    20221 gttttattgc atcttaattt tagtaagagc caaatattaa atggagatgt ttcaagttta
    20281 actgtattga taaataatgt accaataaaa tctattaaat taaatgctaa gacaaattat
    20341 aaaaatacat tagaagtatt agttccaaaa gattatataa ttcaaggata taatgaaata
    20401 aaaataaaaa catacaaaac aatatctgat aaaatttgcc aagatgattc caatactggg
    20461 aactggatgg ttattcacaa agaatcatat atatcaatta gatataagca gaaaaaggtg
    20521 gaaaactcaa taaatgaata tccgtatcca tatgcagaaa ttgaaaataa tcataaatta
    20581 gatacaacta tagtagtacc agataatatg actagaggag agactacagc agtgtttaat
    20641 ttggcatcat catttggaaa aataacaaaa aatgatgatt taaagttaga tgttaaatta
    20701 tattcagaaa tgaaaaattg gtcagatgat aatattatat atattggtaa acctgaaaat
    20761 acagcagaag aaattttgga tattttaagt ataaaggaac aaaccctatt atcttcaaac
    20821 tgtataatca agcaagtaga ttcaccatac aataagaata agaagatgat ggtggtaatt
    20881 ggtagtaatg aagatgattt aataaaggca tcaaatttat taatagaaaa tagactttct
    20941 aaccaagttt tatcatcatc agtgcttgta aacaaagaga ctaatataaa aattaatagg
    21001 gaacaaaaat taaatttagg tcatttaaca ttaaaagact taggatattc tgactttttg
    21061 ttggaaggag catttaatca acaagctttg tttgatgtca aaataccaac gggtaaggta
    21121 ttagatgatg gttcaaagat tatcttaaat ttaagatatt ctgacaattt agattttgaa
    21181 aaatctttag ttacagtgtc tataaatgat gttattgtgg gaagtaaaaa attggatagg
    21241 tcacattcta ataatgataa gttagaatta aaaataccta aagatataga taataaaaac
    21301 tattatcaag ttaaattgac atttaatttg agtataaaaa attcgaattg tgttactaga
    21361 gagagtaata atccttgggc atatgtatca aataattctt atttggcgtt atctacgaaa
    21421 gaaaatgaaa ccttatcttt tgaaaattat ccgtatccat ttgttagaga tgatgagttt
    21481 aatgatttaa ctgtgataat gccagattat tcagggtcac aagctatgac ctggatgttt
    21541 agattaggtg ttactttagg tacgaatata aattcacata atggaaatat aaatgtaatt
    21601 agaggaaaag aatttagtga caagtataag gatactaata ttgtagtttt tggagtgcca
    21661 cataataatt ctgttataaa gatgttaaat aataatttaa atataaagtt cgataaaaat
    21721 tactccaatt ttatatcaaa tgataagata agctttattg atgattatgg aaaaaatatt
    21781 tctaccattc aattaataaa atcaccatat aataatcaaa aaaatatcat ggtaattagt
    21841 tcaatgaatg aaaaaaactt atatttagga atggattatt tgttgaataa aagtaaagtt
    21901 aatgatttaa aaggagatac attgataatt gatgaatacg gagaggtaga agatttagct
    21961 tataatttaa aacctaagaa agaagtaaaa gattcaagct ggaatatgag tataaataag
    22021 acaactaaag tatttttaat gatttctttt attactataa ttgtagtcat gatattatca
    22081 atgctatata taaaaaaata taaaagaaga taatatgata aatttattat tttaaaagag
    22141 gtatataatt tgaggaaata tatgaataaa tttaaattaa aaagaagcat tttaatctca
    22201 ttaataatct taatttttat atttatagtg tactttgtaa gtattgagtt aaaagatggg
    22261 agtctagtaa ataatcaata taaaggtaag attaaatcgg ccaatttatc tgtagattat
    22321 gaaataaatc aagttatgaa ggatatagat aaattgggat tgaatacagt aaatgtacct
    22381 ataattataa atgtagagtc cattaattct gatattatgt caatagataa taatagtaaa
    22441 gaaaaggcaa ttaagttaat aaaaaaatta aataaaaaag gtatttcaac aatattagag
    22501 gcatatccat gggttgaaaa tggaaaattg tatgagactg actggaaccc tataaataaa
    22561 aaaagatttt tttatacttg gcaaaatgcc atattaaatg aacttataat tgatgtagca
    22621 aatccactgg atgtaaatgt tttaaatatt ggttctaatt ttgtacattt ggaagaatat
    22681 caacaaaatt ggggtgaaat aatagatttt gtacaatcca aatttgatgg tcttgtaacg
    22741 tacagaacta attggtggta tacaaaaaaa gatgattcaa gtagtaagtt gttttatgaa
    22801 caaaaattga acaataattt ttttgataag ttagatttta tatctatagc agcatatttt
    22861 gagttaagta ataaacctgt taatacagtg gatgaattaa tttctgcctt acactcatct
    22921 actgtaaaca atagaggaca aaatataaaa caagaaatat ataaccttta taaagctcat
    22981 agaaaaccta tattttttgg agaattaggt ttttcaaata gagaaagtgc aagttctcag
    23041 ccgtggaatc atactccttc aaaagctgta aatggcgaag aacaagcaag gtgttttgag
    23101 gcatataaga aagtttttga aaatgaagac tggataaatg gtttttctgt gttttgtgta
    23161 ggtaaaatag atgacgaaaa aaatttttac ccatcaaaag agagtataaa agttattaag
    23221 tcttggtatg aataatttta aaagtgtaga atttatatag attaaataga tgattaggag
    23281 agatataaat gaatgacatc aaggttatga ctgtatttgg aactagacct gaagctatca
    23341 aagtagcacc tttaataaag gaacttgaaa aaagagagaa tataaaaagt attgtttgcg
    23401 ttacagctca acatagagaa atgcttgacc aagttataga aaccttcaat ataaatgtag
    23461 actatgattt ggatataatg gaaaaaggac aaagcctgaa tgatataaca tgtaagattt
    23521 taaataaact tcccttgatt ttaaacaagg aaaatccaaa tataatattg gtacatggag
    23581 atacaaccac aactctagct acaagtttga ctgcatttta taataaagct cttgttggtc
    23641 atatagaagc tgggcttaga acctatgata aatattctcc ttttccagag gagttaaata
    23701 gacaacttac aggtattatt gctgatatgc actttgctcc tactaatttg gctagaaaaa
    23761 acttgatttc tgaaggaaaa cctaataata atatatttgt aactggtaat actgctattg
    23821 atgctttaaa gatgacaata aaagaaaact ataatcatcc tataattgat gagattggta
    23881 acgataggat gattttgtta acctctcaca gaagagaaaa tttgggtaaa cctatgaaaa
    23941 atatattcag agctataaag cgaatagtag atgattttga agatgtacag atagtatatc
    24001 ccatacattt aaatcctaaa atacgaacta tagctgatga gatatttgga aaatttcctg
    24061 aaaaaattca tattatagag cctttagatg tagctgattt tcataacttt ttaaataaat
    24121 cttatatgat aatgactgat agtggaggaa tacaggaaga agctccatca ttaggtaagc
    24181 cagtccttgt gttgcgtgat aaaacagaaa gaacagaagg aattgaagca aagacactta
    24241 aattagtagg tactaatgaa gatagaatat ataattcagt gagcgattta ttaataaata
    24301 aagatagcta tgttcagatg agtaaagcat ctaatccata tggagatgga aatgcaagca
    24361 aatatatagt ggatataata attaaaaaat ttaattgcaa atatttaaat tagataaggt
    24421 ctaaaacatt aactaattga tataagattt atgataatat taaggaatac aatttttaag
    24481 ggggagtaat atggctagaa aattaataaa aaatttaggt aaaagtaaaa gtgtaaagag
    24541 agtgaaactt ttatttaaga agatttttat tactgtattc atagtagcaa gtatagttgc
    24601 tatttttaat ataacgaaat attttgagga attatacaaa gtaagggatt taaaaagtac
    24661 taaaattgaa tactatatgg atgtggctga tgaagcagga gatggaaagg tccaattaag
    24721 ttggaaggcc ttacttgcta tagacatggt aatacatgat gaagatttaa gtaatataaa
    24781 aaagaaagac acattggata taggggaaaa gtttatagta gaagataaaa atgataaagg
    24841 cgaaaaggtg tataaggtaa aaaagtttaa taaggtatta agcgaattga aatttgactc
    24901 ttctcaaaaa agtagagcaa gaaaatacat gaaagatttg gaatacacat accttggaaa
    24961 taaacaatta gatagtagtg atgaaaaaat taaatttata aagaagttag aagactcagc
    25021 tataagagaa tatattgatt atggaatatt gccctctgta acaattggac aagctatatt
    25081 agaatctggc tggggaaatt ctaaacttac aaaacagagt aataatttat ttggtataaa
    25141 agcagataaa gcatggaaag gaaaaagtgt agaaatttca acttcagagc attataatga
    25201 aaaaattgta gctagtttta ggtcgtataa ttcattacaa gattctgtca aagaccacag
    25261 tttattttta attaataata aaagatatag aaaacatggg ttgtttgagg caaaagatta
    25321 tattagtcaa gctcaagcat tagaaaatgc aggatatagt acagcagaag ataaaaaagg
    25381 aaatcgtata tatgcagagc tattaataga tattattagg agttataatt tgcaattgat
    25441 agacaataaa gttgagacaa aataattgtt aactttatat gaggagataa caagataaga
    25501 ttcttttcta caaaaagaat agaaaaattg taaaaaaaac taaaaaaatg tattgacttg
    25561 ttatttaaaa ggtgatatta ttattactgt actcgaaaga gaacaaaa
//
LOCUS       DAMKPG010000057        25379 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMKPG010000057 DAMKPG010000000
VERSION     DAMKPG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25379
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.060"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            145..819
                     /locus_tag="PC621_002331"
     CDS             145..819
                     /locus_tag="PC621_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109480.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            807..1967
                     /locus_tag="PC621_002332"
     CDS             807..1967
                     /locus_tag="PC621_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4109481.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            2184..2711
                     /locus_tag="PC621_002333"
     CDS             2184..2711
                     /locus_tag="PC621_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF4109482.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            2997..3665
                     /locus_tag="PC621_002334"
     CDS             2997..3665
                     /locus_tag="PC621_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109483.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            3668..6136
                     /locus_tag="PC621_002335"
     CDS             3668..6136
                     /locus_tag="PC621_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109484.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(6376..7800)
                     /locus_tag="PC621_002336"
     CDS             complement(6376..7800)
                     /locus_tag="PC621_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109485.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(7819..8511)
                     /locus_tag="PC621_002337"
     CDS             complement(7819..8511)
                     /locus_tag="PC621_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109486.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(8566..9342)
                     /locus_tag="PC621_002338"
     CDS             complement(8566..9342)
                     /locus_tag="PC621_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF4109487.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(9339..10109)
                     /locus_tag="PC621_002339"
     CDS             complement(9339..10109)
                     /locus_tag="PC621_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF4109488.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(10106..10810)
                     /locus_tag="PC621_002340"
     CDS             complement(10106..10810)
                     /locus_tag="PC621_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF4109489.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(11065..11577)
                     /locus_tag="PC621_002341"
     CDS             complement(11065..11577)
                     /locus_tag="PC621_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109490.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            11903..12160
                     /locus_tag="PC621_002342"
     CDS             11903..12160
                     /locus_tag="PC621_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109491.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            12303..12929
                     /locus_tag="PC621_002343"
     CDS             12303..12929
                     /locus_tag="PC621_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF4109492.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(13179..13958)
                     /locus_tag="PC621_002344"
     CDS             complement(13179..13958)
                     /locus_tag="PC621_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF4109493.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(14026..14487)
                     /locus_tag="PC621_002345"
     CDS             complement(14026..14487)
                     /locus_tag="PC621_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4109494.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(14463..15140)
                     /locus_tag="PC621_002346"
     CDS             complement(14463..15140)
                     /locus_tag="PC621_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4109495.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            15373..15759
                     /locus_tag="PC621_002347"
     CDS             15373..15759
                     /locus_tag="PC621_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4109496.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            15762..17561
                     /locus_tag="PC621_002348"
     CDS             15762..17561
                     /locus_tag="PC621_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF4109497.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(17916..19790)
                     /locus_tag="PC621_002349"
     CDS             complement(17916..19790)
                     /locus_tag="PC621_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4109498.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(19795..21459)
                     /locus_tag="PC621_002350"
     CDS             complement(19795..21459)
                     /locus_tag="PC621_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF4109499.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(21473..22294)
                     /locus_tag="PC621_002351"
     CDS             complement(21473..22294)
                     /locus_tag="PC621_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF4109500.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(22393..23157)
                     /locus_tag="PC621_002352"
     CDS             complement(22393..23157)
                     /locus_tag="PC621_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4109501.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(23154..23903)
                     /locus_tag="PC621_002353"
     CDS             complement(23154..23903)
                     /locus_tag="PC621_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4109502.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(24407..25012)
                     /locus_tag="PC621_002354"
     CDS             complement(24407..25012)
                     /locus_tag="PC621_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4109503.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            25191..25289
                     /locus_tag="PC621_002355"
     CDS             25191..25289
                     /locus_tag="PC621_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDF4109504.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         9099 a         4093 c         3100 g         9087 t
ORIGIN      
        1 tcatccttga tttatttaat atataaaaat atactaactt taatgattaa gttttgaaat
       61 ataactaaga atttgagaat ttattgagat ttttatacta aaatataaat actatacaaa
      121 cttaatttag aagggagcat tcttatgaac aataaaatac tcgtcgtaga tgatgaggaa
      181 ggtataataa cactcttaaa agattacttt gaaatgaatg gttatgaggt ttacactgct
      241 caaagtggaa aagactcatt aaaaaaaata tccaaaaatc cagatatcat tctacttgat
      301 ataaacatgc ctgaaataga tggaattgaa gtttgtaata gaattagaga ttatgtatca
      361 tgccctattc tatttcttac agccagaatt gaaaatactg acaagataaa tggttttcgt
      421 gctggtgctg atgattatat tgtaaaacct tttgatttag acgaacttgg agctagagta
      481 tctgcacatt taagacgtga acaaaggaag tcaaatcaaa ctaaaattaa attttataat
      541 gatttaacaa tagattacga aaaaaggtta atttgtatat ataataatca gatttcactt
      601 tcaaaaaagg aatttgatat agtagagctt ctctctatga atcctggaca ggtatttgat
      661 aaagaaaaaa tatatgatag agtctgggga tacaatagca atggaaatag tgatgttata
      721 atggaacaca ttagaaaaat acgaatcaaa ctatcaaaac atactcttga aaattatata
      781 gaaacagtct ggggagttgg ttataaatgg ataaaataag taatatgtca ttaaaaaaag
      841 ctttctttgt tcttacttta tcttcactaa ttatagcatc agtacttact actttcgcat
      901 acattttact taataatcta tataattcta tacaagataa gtatttaaaa acacctataa
      961 atacaggtat tataaccata gttcaaaaca atgtgaacca atttagtgat tatgataaaa
     1021 cattacttaa tataatcaac atacttcagc ttgtacttcc acttatattt tttataggac
     1081 ttttattatt agctgatata attttttaca aggtaaaatt aaaaacaccc atagaaatcc
     1141 tcaataaagg tgcaatggaa atatccaaca acaatttaga tttttgctta gaatataata
     1201 ataatgatga attaggtaat ctatgtaatg cttttgaaaa aatgaggtct gaactaaata
     1261 aaaacaacat aaatatgtgg actatgatta atgatagaaa acaacttaat gctgcttttt
     1321 ctcatgattt aagaaatccc ctaacagttc taaaaggtta ctcaaactat ttaacaaaat
     1381 acataccaac aggcaaactt agtgatgaga agattttatc aaccacacaa cttatgtctg
     1441 agcatattaa cagaattgaa tactatgtag aaaatatgag taatgctcaa cgattagaag
     1501 atttagttgt ttcaaaatct atgtcgaata taaacaagtt tatagaaaac ttagatgaaa
     1561 atatatctat cattgctaaa caagaaggaa aatcttttac acttaaaagt caaataaata
     1621 atataaatct attttttgat gaaaatatca ttcttagagt cgtcgaaaat ataatatcga
     1681 atgcttttag atatgctaga aataatgtat ccatacttat atatcttgaa caagaactcc
     1741 ttacctttgt aattgaagat gatggtatag gatttagtaa ggaatctcta aaattagctt
     1801 taagaccttt ctatagagat aagactttaa atgacagcaa tccacacttt ggtatgggtc
     1861 ttcatatctc aaaaatatta tgtgaaaaac atggtggctc tatttctatt gaaaataatt
     1921 ctactaacag tgctaaaatt actgctaaat tctcaacaag aaattaaaat gattttgtaa
     1981 gctttcattg ttcttatata agctttgtta gtttagaatt tataaaatgt atttcaattt
     2041 acaaccaata tatatactat tttgtagaag ttgtaagagt tgcttcaatt ataatatttt
     2101 ggcaaaaggc atttgaaaca ataatagaaa tatatattta aatatgtaga atagtatgaa
     2161 actagaattt tggaggtgat tatattaaaa gttttgctaa taatattaca ttctctcgta
     2221 ttgtgtttgc attgctaatg cttagttcta cattctgttc actgcctttc tggatatgtt
     2281 atttttggtg tggaattagt gatatacttg atggtcttat tgcccgaaag ttgagacaac
     2341 aaagtggaat aggtgcaaag ttagatagta ttgctgattt catttttgcg gttacaattt
     2401 tggttactgt cggaaaaaat attgctcttc ccatgtatct atggtggtgt attgttgcaa
     2461 tagcattatt acggttgatt agttacagta ttggtttttt taagtatcgc acattttcat
     2521 cgcttcatac tttgatgaat aaaatcactg gaatttctat ctttgctttt ccattactct
     2581 atatttgctt tggtcttgat gttgcaggaa ttatgatatg tatcgtggct tttctttctt
     2641 cttttgaaga aatagcaatt atgataaaat caaaagaatt aaatcgtgat ataaaaagta
     2701 tatttatgtg acaacttaga atttgtgaca aaaataattt atataataat gaggaagtaa
     2761 aacataaaaa attatccaca agtttatgtg taaaatagtt ttaaagcaat agagttgtat
     2821 tgtaaagcct ttagtgcata aattacattt taaattaaaa atgatactga aactgcttat
     2881 cagcactgcg aattatccgt aaatagtgaa gactttttag atttaagcta aaagttggga
     2941 aaaaattgag ttttaaaact tacactcttc ttatatagaa aaggagagtg ttttttatga
     3001 ttataaaagc aaaacaatta tcaaaaatat atggttctaa taacaataaa gttatagcac
     3061 ttaataatgt aaatttaaaa ataaactcag gtgaatttgt ttctgtaatt ggaccttctg
     3121 gaagtggaaa aagcacactt ttacatattt taagtggatt agataatcct acctctggac
     3181 aagtgttact tgatgataaa gatatatata aacacactga aaaagaacta tctgcactta
     3241 gaagaaaatc ctttggattc gtctttcaac aatttaatct tttacctgtt cttacagcat
     3301 ctgaaaatat ttcaatgcct gtacttctag ataaaaaaca acctgataaa gactatttaa
     3361 atgaaatttc atcattactt ggaattgcag atagactcaa tcatcttccc catgaacttt
     3421 caggcggtca gcaacaaaga gttgcaatcg ctagagcact tattgcaaaa ccagatataa
     3481 tatttgctga tgagcctaca ggaaacttgg acagtaaaag tggcagtgaa gttatgaatc
     3541 ttcttataaa aacttcaaaa cagtttggaa agactttagt ggtaattact catgatgata
     3601 gaattgcaaa acttgctgat agaaaaattt caattattga tggtgttctt atggaggtga
     3661 aatagaaatg aagaattacc tttcactttc aataaaagaa ttaaaaagcc agaaacttat
     3721 gactacattc atcattatag caatagtact atcaactatt atgacaactg tggttggaca
     3781 gtcaattggt atcctacaaa atttaagaat tgaacaagcc cgttctttta atggtgatag
     3841 acatgtatcc tttcaccaac ttactaaaaa tcaagttgat gacataaaga aagatgatag
     3901 agtttatcaa gctggtactt caataactat tggcagtagt aaaataaagg attctggtat
     3961 ctctgtactt gtaaaagagt atgataaaac aggactttct aactacccaa aattaatgaa
     4021 attaaaatct ggacatttgc ctaaagataa aaacgaaata gccttagatg aaaacactct
     4081 aaagcttatg ggtataaaac caagactagg agtgactata cctatgaatt tagatatttc
     4141 acttttaaat gataccatac caccatataa ttacacagct aactttaaat tgagtggcat
     4201 tttagaggat gattatacag gatatgtaag tggtatagta aatggtattg taggtaaagg
     4261 tacatcagaa aatctattac ctaagagata tattttatcc tctttggatt ttaaaataaa
     4321 acagcaagaa aagtttcaag aaatagtaaa tcaattagct aaaaaaataa atctttctca
     4381 caattcaatt caatataatt ggatttactt aaatgctctt aagatacagt ttgaaaaaga
     4441 tgaaaatagt tctaatagtg atggtatatc tatgattata ttggtatctt tatttgttgc
     4501 cttattggtt ttactggctt ctggacttgt aatctataat attttaaaaa tctctgttac
     4561 aaagaaaatt aaagagtatg gatgtttaag agcaatagga gctgaaccaa accagatata
     4621 taagatagtt attttacaaa tcttaatttt atgtactgtt gccataccta ttggagctgt
     4681 aattggaatt atctcttcta aaggcataac tggaatggtc actaatatct taaatccaga
     4741 tatacttcta gcaaatgata ataaagaaat tactgagctt atacataaaa atacaacagc
     4801 atatatgttc ccattagtat taagcactaa tgtatctttg attttttcat ttatttcagc
     4861 attaccatca gcaatatatg cttcacgtgt ctctcccaaa attgctatgg ctggtagtac
     4921 taccaaaatc aaaagaaaaa ttaaaagaga aaaagcaata aaaaactttg aaagacatct
     4981 agcatggcta aacttaaaaa gaaataaagg aagaactata ataactatac tatcattatt
     5041 tatgagtata acagtattcg ttgcattgag tgagttctct aatgtattag atgtatctag
     5101 gtcagtaagc aatttaaaag aaggagattt ttccttaaca aatgaaatat ctggttttga
     5161 taaatcaaca ctagataaaa tagataaaat gaaaaacgtc aatcgcactt cctttataaa
     5221 gtactctgaa tacaaacagg gtgaaataga tacagatata aattttgaaa atagtggtga
     5281 gatgttaaaa atcattggaa ttgacgagca gactttaaaa gacttaatgc cttctattac
     5341 agattctata ctagaagact ttaaaaatgg ttctatctgt tttataaaaa atccgattgc
     5401 tatttctaca ccaggtgtaa aaactagaca tacaaattta aaacctaaag atagtatcac
     5461 aatcaacaaa aaacaactag atatatatag tacagtagat aaaatgttct ttttacaagg
     5521 taatggctgg ataaatggtg ttgatgttat tgtatataat tctgtttata acaccttgac
     5581 aaataaaaac aagttcaacc agataaatat ttatgctaaa gataaatcaa aattagaaca
     5641 aataagattg tctattgaac agatttgtga aaataatcct ggctctcatt ggatatcata
     5701 tattgaatca gataagcaac ttaaagagag ttttaaacaa atagaatttc tagcatgggc
     5761 agttatatta tttgtgggat tgattggtac tttaaacatc attaacacaa ctcatacaaa
     5821 tataaatact aggactaatg agattggagt taaaagagct attggtatga gtaatattag
     5881 cttatataaa atgtttttat gggaaggtgt ttattatgga atttttgctg ctatatttgg
     5941 ttcaattgct gggtatgcca gtgctattat tataaacatg gcaactatag aaaagcttga
     6001 tttcactaat ataccaatta cttcaatttt acaagctact attatttctg tacttgcatg
     6061 tattatagct actcttattc cgttaagaaa agttaaaaag atgaatataa ttgactgtat
     6121 agatatcaat ttataactat tttacagata ataatataaa ttaaattatc aatgactgtc
     6181 tctattttct ttcaaatcta actaataaat ctttttaact ataatataag ctttaaaaaa
     6241 ggcctgtctc aaaatagaga taagcctttt ttcattgtcg gaataatgaa aatatattta
     6301 acttcacaaa aaaagagtgc ctcacgaatt aaaaatttcg ctttgagaca ctccccttta
     6361 atttaaaata tatagttaaa tgctatgcca aaatttcaca catgctccac ctgattttga
     6421 attttctatt ttaacagaac ctccaagctg ttccactaat tgtttgacaa tgtaaagccc
     6481 aagaccagaa tgtccacctt ttgtctgcct agcttcatct cctcgataaa acttatcaag
     6541 agcctttttt aaatctttag agctaaaccc tctaccagaa tcacaaatct cataagaaat
     6601 acaatttttt tcatccttta cagtaatact aatattacca cctgatggag tgtattgcag
     6661 actgttggat atgatattat caaaaattcg tgtcaaccta tccacgtcga tttgaattgg
     6721 ggattgtata ttccccttca ttttcaatat aagttcaact tccttttgat gagcctgtaa
     6781 ttcatagtct tgcacctttt gcctcaaaaa ttcaggtaaa tttataggaa caagatttaa
     6841 ttgtatattt gacttttcta agtcagacgt atattgcatc ttttggacaa gagcagcact
     6901 tttttctgta ttttctctta tcactgtaag atacctatga agtttttcat tgtcatctgt
     6961 attattacca ataagtgcat ctgtatatcc caaaataata gaaagaggag attttaaatc
     7021 atgtgctaga gcttccacca tttcaacacg ttcctgctcc atcttccact gcgcagataa
     7081 agaacctttt aattcgtctt tcatttcaga aaaggcacta caaagtttac cgagttcatt
     7141 atctgaataa tagtcgattt caaaatcaag gtccttttct tttatcttct tggacgcatc
     7201 aatcaataga tgtaatggtt gattgatatt ctttacaaag cgcttagaaa gccatctagt
     7261 aaaacccacg atatatataa atggagaaaa cagagccatt ataataactg caaatacaaa
     7321 tctccctcta ctatttgcaa aggttatttt gacttgatag aatagaataa cagctccctc
     7381 aatctttcca ttatcccctt tgattggaac agtatggata taaccacctt tacgaacagt
     7441 tttattgata aaattattaa ataattcttc ttttgtttta aatagttttt tggggttagt
     7501 accatataga atattgccat tattatccac aacttgatat ggcatatcat ccccacgaat
     7561 cgttcttttt aaaccttctt cattcgattg tgataacaaa gttatgtttt tttcattgat
     7621 gtatttttca attctaggaa cttgtcgctc ataatagttt ggtggataga tatccttatt
     7681 taaggaatga atatacaaat agagagcaaa tacataagta atcactgtgg caactatact
     7741 ggctattata ataaaaataa aagtatgtct aaattgggta ataagggatt gatttttcat
     7801 ttaataacct ctctctcttt acattttatt ccacttatac ccaatacccc aaactgtgga
     7861 aatatattct ttttctggtg tcacagcaga aaacttagct cgtatttttt ttactcgttc
     7921 aacaacagtt gaggaatcac cttcagaatc acacccccat actttttcat agatttgttc
     7981 tcttgagaaa acttgtcctg catggagtgc tagtaattca acaatatcat actctatttt
     8041 tgttaaatca atattcttac catcaatttt aattgcacgt tccttcatat ataagcataa
     8101 cttcccaaaa tacaaagtag atctcttgtt ctcttcattg atatactgtg aacgcttttc
     8161 cctacgcaga ttagcttcaa tccttgccat aagttcacgc agtccaaatg gctttgttac
     8221 ataatcatcc cctccaaagg tcaacccttt gattttgtcg gtttctgatt gctttgcact
     8281 taaaaatata attgggcata aaacatcatc tctaattgcc tgacatacct caaatccatt
     8341 cattttaggc atcataatat ccagtataat aaggtcaggc tcctgttttg ataactctat
     8401 acctgcctga ccatcataag ctatcaacac ttcatgtcct tttgccttca attcatcctc
     8461 aagtagcatt actaggtcca tttcatcatc aataatcaat attttactca atataacacc
     8521 acctttctct tatgggaagg tcagtattaa aacaatattc gtctactaat aacttttcct
     8581 tccctcccac attttaaacc aaataacgcc tcctatcaag aatataatca aacctattgc
     8641 tactactata agctctatac tagctgtttg catcacttta gagtaaaatg cctcagttga
     8701 aatagttcca gcttctttaa taaaataaat tcctggaagc atacctagtt ttactggcca
     8761 tgtccatgga acaaatgccc atactttttc accaacgcta gttaatccaa gtattgctgc
     8821 catcaaaatt ccaccgatac taataccaat agatgcaccc ataccccatg caaaactcac
     8881 ccaaagatga atagctaata gtggaagtgt cccaatcacc acaagtcctg ctgctgacag
     8941 gaataaccat attgttgctc cagatggcac tgcaatactc attcctatgc aaagtatcaa
     9001 cgtagcaata aatgtgcata tagttaggca aaacagaaga aaaagaaatt ttcccagata
     9061 taatctaaca cgagaaatac ctacacataa aaaaccatta aaatttccag caagctcttc
     9121 ctcctgcact ataaatcctg ctaaaatacc tactccaata ggaatgatga atacagtcca
     9181 tactgtaaaa aatccttcaa aagcaaattc ttgtgtactt ccagatttag ttgtgagata
     9241 aaaaaccaca cataatgaaa aaataatagg catacaaaga gtaagccact ggacagctgt
     9301 cctctttgct cgaagccatt ctgatgataa tagctttatc atttgtcatc cctcctattg
     9361 aaccaagctg cagtaagcgc tgttaatact ataaacacaa ccaaagaaac aacaatccca
     9421 ataggaatca ctgaagtatt caaaagtggg tctccagctt ctaatatagt accgttagga
     9481 tgtatgttga tgattgggca taccattcta actgcccaac tccaaggtac tgcaatccaa
     9541 aatggttttg tcgcagcaag gacaccaaat atcataccta aagctccaac tcccatactt
     9601 aggaacatac ctttccatgt agctagccat agctgaagtg gaagtatagc aagagaaaca
     9661 acccagcata caatacttgc ccctataatc tgtccaaaag gaactggacc agctttcaat
     9721 attaaaccag ttataattgt tacgactatt aaaactaacg ttgaaataaa actgtagact
     9781 gccatagcta taactttatt aatccaaagt gtcataggag acacattgtg tgtacgtaaa
     9841 gcacgataat ttccagcttt tttttcctgg aaagctacca aagtagcaaa caatgcaaat
     9901 ccaataggca gaaataaaaa tgaccaccag ttaaaaatta atgctaaaag actttgccac
     9961 gcccaagaag ttgtttgaga taatggattt tgcatgagtg ttgattgcat tataaatgca
    10021 tatgctgcaa aagacactgg gaaaaaaaca ataagtttac ccataaatgt tcttttatac
    10081 tttaaaagtt ctgattgtaa agcattcatc tgttttgtcc tcctttaaga ataacatctg
    10141 taaaaaattc ttctagattt tcattagggt caggcattcc ttgaaatagt agtcgtcctc
    10201 catttataat tcctatatta tctacaactt gagcaacctc tgatagaata tggctagaaa
    10261 gaattacagt tatacctttt tttggaaaag aagcaatcaa ttcacgcaat tcttgaatgc
    10321 caaaggggtc aagaccattg gttggttcat ctagtattaa caactctggg tcactcaaaa
    10381 gtgcaatagc tattcctaac ctttgtttca tacccataga aaattgtgaa gctctctttt
    10441 tacctgtatt tttcaaattt actatttcca acacttcata aattttatct ttaggtattc
    10501 ccaatatttt tgtatgtact aacaaatttt cctctgctgt aaggtttcca tacaatgcag
    10561 gggattctat taaagaacct atttttgcaa gagattctcg tttccaaggc tttctatcaa
    10621 aaataatacg accatctgtt gggcgaagaa gaccaacaag cattttaagt gttgtggact
    10681 ttcctgctcc atttggtcct aaaagtccat atatactatt tcgttcaatt tgtagagaaa
    10741 cattgttaac tgccatttgt tctccatatc tttttgataa gttttttgtt tcaagaatta
    10801 aatctttcat attaatttct cccttcttgt aataccttaa atctattact aagagaatac
    10861 taacatgaaa aaataacaat tctataacac ctactaattt taataaaaat taaaataaaa
    10921 aatagagtta ccaattttat tttggcaact ctatttaagt ttatgtttta cagttatatt
    10981 agatttaagc ttagtttcat tatcacacta acttcaaatt tactctataa tccttaaatc
    11041 aagcactaat ttattaatca aacactaatc tattaattta tatttcttta gacatctaat
    11101 atagtttgct actaaataat aattacttat atcatattta tctaatcctt taaaagaagt
    11161 tatttcatca ttaagctcat cactaccttc tacaaaaaca caacctgttt caaattccat
    11221 acctacaata atcttacagc cactataagt ttcctcttga gtatcgtaca atttttttgc
    11281 atttccttca tttggaattg taaatacaat aggttttaat tctttatcac tggaaatctt
    11341 ttgtgaaaaa ggcttgttta acttaatatt attgataact acatgtttaa attcctctga
    11401 agtttcttct actattacat attttcgctt taaaaactct attatttcta aaacagactt
    11461 agaatttact tttaaaatac ttttatattt ttcatataaa gatttccact gttcaatttt
    11521 ttcctgtggt acttcttgga tatgtgtact tattttttct tgagatattt ttttcaatta
    11581 aactctcctt tcaatactta aatatcctta taaatctcac atgactaaag ttacaaggtt
    11641 cctacttaat agaattttgc atacttaaat atgtcttatt aattctctac aggatatctc
    11701 cgtagtccct acgattctta cataagttac ctattttaaa ttgttattag ttagattata
    11761 aaaattattt attttatcac taatataata ctcttaaaaa ctttacaaat caatttaaat
    11821 ttgctacctt ttatttataa caacttatag tacatttctc ctatgctttt caatatatta
    11881 cttttaaaag gaggttttcc ttatgatttt aaatagaact gagcttttcc gaggatattc
    11941 tttaaataga tttaacgaga tatgcaatat attatcagta aataaaatta aatataagta
    12001 taaagtgaaa aataatgctt actctaaaaa acctttttct aatgaaataa cacttggcaa
    12061 tttaggtcaa aaggaagatt tctcttatga gtacttaatt tatgttcata aagacgatta
    12121 tgaacaggca gagtctctaa taaattctaa gttgcgatag attatttata aaacatgaca
    12181 ttctgttgtc atgttttata tgttattata ctttcaaata tgctactata ttttcaaaat
    12241 tgtgtgcttt aaaactagct ttttatagta atttaaagct taaaactaag gagactcgtt
    12301 atatgaaaat aaatataaaa tcaaagttaa gtgaatttat taaacagaac aatctttttg
    12361 atgacaacag aataatatcc taccttccta atataacaat agaaacagac aaaataaaaa
    12421 ctataatgat taatgaggtt gtcccaacta acaccaatga cgatttttat agtgtagata
    12481 aagatgctga ttatttaaaa actactattc ctttatttga cagtgcagga atagaagtat
    12541 ctaacataaa tgatatccta gatatgggta tctatataac taatgcagta aaacttccta
    12601 aatcagaata tacaataact cgtgatacaa ttaaattaca tatgcctatt ttagaagaag
    12661 aaattaaatt atttaaaaac ctagaggtag taatgttaat gggagatgtt gcaaaaaaat
    12721 cttttaatat gattactaaa aagcatataa aaaagaatgt catcccttcc atttcaacat
    12781 ataaacttcg taataatgaa ctttattatg ataatttaag agtatttcca tcatatatta
    12841 tgactggtgg aaacatactg attgaaaaat caaaatttga aatggcatca gaagatataa
    12901 aaaaaatgtt cgagattata aacggttgat attacaaaat aataaaaact aatttagcca
    12961 aataataagt gcctcaaaaa cttctattta tgaggcactt ttattttata ttagtgtaac
    13021 tatatactat taacataatt tattatttat attgttcttt ttaatctcta ttccatttag
    13081 tctaggtaac attactcttt acaagattac tttatatttc ttttatatct ctaacgtttt
    13141 tatttaataa aacaattatt aatacaagtc aaatatatct atttatctaa aaatatatct
    13201 tttacagtaa tatctggatg gtgatacttc ttcctattcg ttaaaaaaga tttttttaca
    13261 ttaatagcct cttttggaca ccagtgaaaa caagctaggc atctttcaca ctcattaccc
    13321 cactttggct ttttatcaac tatacttata ttctctacag gacaaaccct tttacatgtc
    13381 ccacatccat tacatttatt ttctgcaaaa aatttagtag ttgcaatttg catattctta
    13441 cctactaaac gaagtacaac actatctata tgtgacttta aattgttttt tccttctata
    13501 tttccaatct ccaagttatt gatacatttt gcaatctcag ctaccctatt agtctgattt
    13561 ttcaatcttt cttcttcaac atctgaaggt gttatcaaag catttccagg catattgatt
    13621 aaaaaaccag cagataattt tttccccctt tttttaagta acttatctat agtaaataaa
    13681 ctatgtccag ctaccccatt acaagtggca atggcatata tatgagtatt aatattaaaa
    13741 tttaatttct ctacaaaaga ctttattata tcaggaacat cagcaaaata aacaggaaaa
    13801 acaattacca atatatcttc atttatgtct atattatttt cttttctatg tatagctaaa
    13861 ggtataactt ctttacttcc atttaacgtt tcttttaaaa tctttgaaac tactagcgaa
    13921 tttcctgttc cagaaaaata ataaatctta ctattcattc ttatccctct ttctatattt
    13981 ctacatcatc aatagttaaa aagtctctta aaataggatt tatatttact ttccaattct
    14041 atccaaaata tcagtaaaca aaggcaattc ttcatcagag atagatttaa atgtctctgt
    14101 tgcatatata cttgcaattt catttaatgg ttcttctaat tctctaccta aatctgttag
    14161 gaatatttga aacttacgtc tatctttaac atctacttta cggacaatta atcccttatt
    14221 tttcataacg tctaaaattc ttgttgtatt agatttatcc ttacatgatt tttcacataa
    14281 atctatctga gaaattccat cttgctccca tagaaacctt aataacgcat aatggtcagt
    14341 tgttatatca tatccatttt gtttaaacat cttttttaaa tcatctttta cttttaacga
    14401 agtacttact aacttagttg tagcttcata tagtttctca aaatctttca ttttacctcc
    14461 atttaaataa tcccctgctc ctcccatcta tcaattattc ccttatattg ctcaatatct
    14521 ttaaaagcca ttggctttaa aacagcattt atagcaataa aattatagat ttgagaaagc
    14581 acaggtggat ttttcttttc ctctatgcaa taaataaatt ttttagatac cttttgtatc
    14641 tttgattcta ctttttcagt tataggattt tgattaaatc cagtttttat agataaacta
    14701 ccacaaacat aacctcccga atctaagacc atggatttta taaaagaaat tgcttttgaa
    14761 ccacgtgcat tttcatatgt agtaactgct atacatggct tacgatacaa taatctttca
    14821 agcatcatat gtgctctatc tgtaaacacc ttgaaaagtc cagttacact acttccataa
    14881 gttgggctac caaatataac accatcactc ttagtaatta tatcatgaat atactctact
    14941 ttatcatttt gattgataca cttgcccatt ttataacaat ttaaacaacc aatacaatat
    15001 gatggattta cctcacttaa atcaaaataa ttaattttta catctggtat taatctttct
    15061 atatctttat acaagtaagt taaaactttt gatgtagctc cattttttct agggcttcca
    15121 tttataactg taattatcat ttctatatct cctttttagt tgttatatca acaataagta
    15181 tatcaactat atttttagct gtcaatataa accatttatt taaagctata cttacacagc
    15241 ttaaataaaa gttttattta actacatgtg cttatttaat caactataaa tgaattattt
    15301 ttaataattg acaaactaaa ttaatgataa tatactttat gtattacaaa tgtaagtaga
    15361 aggaggtata gtatgaaaaa gttaccacat atttcagaag cagaatatca agttatgaaa
    15421 attatttgga aatatgctcc tatcagcact actgaagtaa ttgaaaagtt ggtagaaaca
    15481 agtacatgga gtcctaaaac aattcaaaca atgttattga gattagttaa aaaaggagct
    15541 ttaacatatg aaaaaaatag cagagtattc gtttatactc cacttgttaa agaagaagaa
    15601 tacgtagcta cagaaagtag ttctttttta aatcgttttt ataatggtac tcttaattct
    15661 atggtactaa actttttaga aaatgataaa ctatctgaag atgatattga ggaactgaga
    15721 gaaatcttaa ataaaaggac gactaagggg gataaataaa tatgtatgcc ccattcttta
    15781 tacgattttt actcagtaca ttaaccttat caatattaat tgttataatt ttacttgcca
    15841 aaaaagtatt taaaaaacat atttctgcaa agtaccaata caatattgga tttatattgc
    15901 ttataatgtt aataattcca tttgtccctc ttaaatatat taatcttgga aatgtattta
    15961 actatttata tacttttaat caacttggaa gcagtactaa taggacttct attataaaaa
    16021 atagtccaaa ttctataatt aataactcaa atgcattaaa cgatttttca gtatccctaa
    16081 accaagttaa ttttgatagt ttaaatattt tattactaat atcatggata attggaattt
    16141 taatcatgat agctattact attcattgca atattaaaat aaaaaatatg aaaaaatcaa
    16201 tacaaatttt aagagatgag gagatagtat ttatatttga aaagtgtaaa aaagatattg
    16261 gtataaataa ggattttata ctatgcaaat cacctattat aaatacacct attacatttg
    16321 gatactttaa accacacatt atattaccag ataagtctat atctaaatta tctttaaaag
    16381 atataaaata tatcttactt catgaattac aacactttaa aaataaagat atactgataa
    16441 attatataat gtgcttcctt caaatattat attggtttaa tcctcttgta tggtatgcat
    16501 ttaaagaaat gcgtatagac cgtgaaattg catgtgatat ttcagtttta aaaaagcttg
    16561 ataaagatag tcatatagaa tatggacaaa ctctaataaa ctttgtggat aatatttcaa
    16621 gaccatcaaa tcttacactt acatcagata ttggtgggtc aaaaaaacaa ataaaaaagc
    16681 gactattgaa gataatggat tttcataatg aatcaaatac gttaaaattc aaaagcattt
    16741 taatttttac attgatagct ttaattgtat ttggaaatat tcccaaacta tctattatgg
    16801 cagatatcaa cgaaaaatat agacctacaa atgaacaaac tgtgtacgag gacttaagtc
    16861 agtattttaa aggttttgat ggaagttttg ttatgtatga tttaaaatcc aagcaatatc
    16921 atatttacaa taaagaaaaa agtaaaacaa gaatttctcc aaattctact tataagattt
    16981 atagtgcctt attgggattg gaaaatggga taataacaag aaataattca catatccctt
    17041 gggatggaaa gcatcattct aatagttctt ggaatgaaga ccaagactta ttttctgcta
    17101 tggaaaactc cgtaaactgg tactttcaga ctctagacaa aaaaacaggg ctaaaaaaca
    17161 tacaaactta tcttaagcaa attggatatg gcaattatga tgtttcaggt ggtatctcaa
    17221 acttttggct agaatcttca ttaaaaatat cacctattga acaggtagaa ttacttgaaa
    17281 gtttttatac aaataagttt aaatttgaag ataaaaatat agaaacagta aaagattcat
    17341 tgttaatatc aaaaaaggat ggggtatcac tttttggtaa aacaggcact gctaatataa
    17401 atggaaaaga tgtaaatggt tggtttatag gttatgtgga aaaaaatgaa aatacttatt
    17461 tctttgctac aaatattcaa agcaaagatt attctaatgg tagttcagca tctaagataa
    17521 cattatccat attaaatgac aaaaaaatct ataatcctta atatatttac tgtacattct
    17581 aaaaaattca cataattttc atattaatta tcgattaaat tcatatacaa ggcttattat
    17641 ataaacatag aagatagtaa aactagctta aaaacataat ataataccct atacaaatag
    17701 tggcaactct ccctcacatc ctcaattgtt gccactattt aattttttgt atgaagttaa
    17761 atatattttc atcatttcaa caatttgggt gcagtacaat ctctattttg agacaacctc
    17821 tttaattgtt ttagagttta tgaattattt ctcatacttt aaaaagattg aattactctt
    17881 gataatcttc ttttgtagtc attacatatc acctactact caacacatat tctttgacct
    17941 atttttacat ccttgtttat atccaaaata tttaccttct taccatctgg aaacaatacc
    18001 attgtcaaag tagactttcc taattcatgt attctcttta aatcaacatt aacaagaaca
    18061 tctccctgtt taaccatatc tccttgttta acagcaatat caaagccatc tccatttaat
    18121 gcaactgtat caattccaac atgaattaat acttctccat tagttccttt aatcccaaat
    18181 gcatgtccac ttgaaaatac tgtttctaat ttacctgaaa taggagaaac aatttttcca
    18241 tctataatat caatagcaaa accatcacct aacatttttt tagaaaatgt ttcatctggt
    18301 acattttcta atgcaacaat tttcccctta gctggagaca caaagttttc attattttca
    18361 ttaaacacta attcttcgtc ttcatcttca tttatagctt ctttaggcac tccaaataaa
    18421 catgttacaa taaagcagaa tgcaaccaca aatattgata ctaataagta tgtgattaaa
    18481 ttatatccat catagatata cattaaataa gaaggtaaaa ctgctaatcc atatgaatta
    18541 gattgaatat gtaaaattga taatattgct gctcctaaac ctgaagatgc acacataata
    18601 atcaaaggct ttaaattata tcgaagctga atcccaaata aaacaggctc acttatacca
    18661 aaaagttgag atagcattgc tccaactgca gtttgtttaa cttgattatt tttagatttt
    18721 ttcataaatg ctagacatgt tccaatattc gcaaatccat acattgcaca aagagtaata
    18781 agtgcattaa atcctgtatt tgctaatagt gatgtttcaa tcattacata agtatgatgt
    18841 aagccagtaa taacagctaa tggatatgta aatccaataa taaatcctcc aattcctaaa
    18901 ggtaaaccaa tcaataaact gatgatatca accattttta attctattgt atgcattata
    18961 ggccctacac ctaacaaaat aactaacatt gttattagca taacaaagaa tggtgtgaaa
    19021 ataaggtcta atacattagg catatgttta cggaaccatt tttcaagatt agctccaata
    19081 aatgctgtaa caatagctgt taaaacactt ccttgacagc ctacaatagg aataaatcca
    19141 aatgccataa ttgcttcaac ttcatttgat gggtcagcaa cagaatatgc attaggaagt
    19201 attggagaaa ctaacataag tccaataaca agtccaataa caggtgttcc tccaaatacc
    19261 ttaaatgctg accatacaat aattgctggt aaaaaagcaa atgctgtttc tgtaagaacc
    19321 gaaacaagtg tctgaatata caatggaata tttgcagaag acattccaaa taatccaagt
    19381 acattatcat taaacaaaca accctttaat cccaagaata atcctgttgc cgcaataact
    19441 ggaataatag gaacaaatat atctgctaga gttctcataa ctttcttaaa tccagtttca
    19501 ttatttttaa ctaattcatc ttgttctttt tttgataaag ttttcaatcc cattttttca
    19561 acttctgcat aaactttatt gactattccc gttcctagaa taatttgata ctgtccagaa
    19621 gtaaagaaaa cacctttaac ctcattaacc ttttccactt tcttatcatt aataatttct
    19681 ctatctttaa ccatcaccct taatcttgtg gcacaatgtg taatagaaat aatattattt
    19741 tcaccaataa tttcaataag ctcatttgct attttattat atttattcat ttttctacct
    19801 cactttcaat aatactgctt gaaatggttt caaactcact gttttattta gtagatttat
    19861 atcatctaaa ttttggaata caatatcata tttttcttct gtttttattt ctacaagttt
    19921 atcagaaaaa ttataataac aatcaataac taccaaatct aattgccttc tatacgcaat
    19981 aacattgtca ctttctatat caataggttc tattgaacca taaatcaaac aatcagaata
    20041 cttactatct tgtctcaact ttatcatctt tttataaaat tctaaaatac tttctgaatt
    20101 gttaatctga ctctgtgcat taatatcttt ataattttca ataaccttta accatggtgt
    20161 tgcatctgaa aaacctgcat tttctttatc tgcccatgga aatggcgttc ttgcattatc
    20221 tcggcttctt aaattcacaa attctaacgc ttcttcttct gagtaacctt catctattga
    20281 acgatagtat tggtcaatgc tagataaatc attaaaatca tttatggtat ctcttttaaa
    20341 atttgtcatt cctaattcct gtccctgata aataaatggt gttcctctta aaaagaaata
    20401 cattgctccc aacattttta ctgcatcatc attcctttcg tattgcaaaa gatactttgt
    20461 tgtagctctt ggttggtcat gattttcaat aaaattagct gcccatccat gttcctgaac
    20521 atacatttgg ctatctaaaa tgcattttct taaatctgga attgtccatg aaattctttt
    20581 aaaccactca ctaccagaag caacatctaa gtcagcatgt ttaaagtcaa agaccattga
    20641 aaagaaacca tcttctccaa taaaatcacc tagttggtca tagctaatac ctgctgcttc
    20701 agcaacagtc atacaatcat attttttaaa cgtattttct tttaattcat aaagcatttt
    20761 ttctattcca ggctgatttc taactgtttt tgtacattta acaagaccat caacaccatc
    20821 actttctaaa ctttgaaaac ttaaattctt tttgataaat gtaatagcat caactctaaa
    20881 tcctgccact cccatttgag accaggtatt aatcatttgg taaagttcac ttcttaatat
    20941 tggattttcc caattaaggt caggttgttt tttaccaaaa gaatgaaaat aatattcgtc
    21001 tctattctct accttttccc aaacacttcc accaaaaaca gacctccagt tatttggtaa
    21061 atcgtttcct tttttaaaaa tataataatc atgataagga ctttctggat tttttaatgc
    21121 ttcttgaaac caaggatgtt catcacttgt atgattaaga actaaatcta atataatttt
    21181 gattcctcta tctttacttt ctttaattaa ctcttttaaa tcttccatac ttccaaattc
    21241 acttgcaata tctaaataat cactaatatc atacccatta tcgtccatag gtgatttaaa
    21301 aattggacat aaccacaatg atgtaatacc taaatctttt aaataatcta aacgagaaat
    21361 aattccctgt aaatctccaa ttccatcatt attactatct tgaaagcttc ttggataaat
    21421 ttgataaacg atttcttttt gccaccattt cttttccata aattactcct ttttatatga
    21481 tatttcttct ttttaattct ttataaattc cattatcgaa tatgtcctca catatagatg
    21541 tcgctatatc tttaagttgt tgatgactat ttttcattgc aattgtaacg tctgatgctt
    21601 gaaacatgac aatatcattt tgaccatcac caaaacatat ggtttctttt tgagttaccc
    21661 ctaatctttc ttgtaatctc ttaattgctt tgcccttatg aaaatctttt tgaataattt
    21721 cataatactg actagaaata tctctctgtg ctaattccat tttatcttgt agtatatctt
    21781 tcacttcgtc aaataccttt tcatttgacc ataaacaaat tttatgtaca tcttgtgact
    21841 tatactcttc aatattattt tcatatgtta ttttttcttg gatatgttgt ttatttatac
    21901 aagaattagt tcctttcaac tgattcattg tttcaaatat ttctttggct ttttgattca
    21961 tgaaaacttt ttcctgactc tcaatcgaaa aagcaacttc tctctttttt aacaaacaaa
    22021 caacttcctt tatcaatctt tgattaaaag attgattata aagtaattca ccatgatatt
    22081 gaatgtaatt acctcctcct gcaatatatc catcaacccc taatgataaa acatcatctt
    22141 gtattgtacc tatacttctt ccagtacaaa taacaacact gcaatgattc ttttgacata
    22201 aacgtatagc gtgctttgca gattctggga ttccatatac ctcatcccta agcgtaccat
    22261 ctatatcaag tattaataat ttgtacaatt tcatcttcct ttcttactat caaaatcata
    22321 atagcattca aaaaaaaaga tatcaatatt ttgatatctt ttaaaaaata tgttcatttt
    22381 tagcacaaat tttcactttc ggggaatatg ttcactttct aaatacatct tgtatttaat
    22441 ataaagcagt tcaattaaaa taaaataagg tgttgttaca tcgtagtcca gttgtttatc
    22501 tacagaaata gatatagatt gaatatacag gttttcatca cataatcttg ctaaaggatt
    22561 acttatcatt tttgtaatag aaaccatata agttcctttc attctcattt ctttagcaaa
    22621 atcaataatt gtagatgtat ctccattcaa tgagataaag aaaatcatat catctgtttt
    22681 tataaaatga ttaagcgcct gaatcatatc ataaccataa acatcatata taattttccc
    22741 tgttggtaag aaaatccttt tcatctcttt aacaactctt tcctgagaat aaccttctcc
    22801 aaagacgatt atacgatttg cctttttcat tctttcaaac ataatcgaac aatccttctt
    22861 ctctatatca tctatcacct tattataaca atccattaat tggtgtttac tttgattttt
    22921 atattctact tctgtttcat ttaagcgtat aataactctt aattcactat atccaggtaa
    22981 ttgtagtttt tgagcaaaac gagaaagtgc acttgtagat atatggtatt tgtctgcaaa
    23041 ttcatctata gaaagtttaa tacaatcttc tttatgatta agaatacact cacagatata
    23101 cttatcattt gctgtaaatg aattataata tttattgaaa agtccttcaa ttttcattct
    23161 tttctccttc tatactctat gtaattcata tgaagcaaat ctaatagagc ataaaatgct
    23221 cctacgactt cataagatat agcttgtaat gtatcaagtt tttgagtttc aacatacaga
    23281 ttatactcac aaagtgttgc aattgtatta ttatcaagcc tagttaaaga aatcgtatgt
    23341 atatgattgt tgataaattt tgcaatttca attcctgatt ttgtttctcc agacaaagaa
    23401 atgataataa ataaatcact tttttgaaat gattctctca taaattcaat ttcacctata
    23461 tcaaataagt caataacaca tttacctact gaaagaaata ttctctttat ttcttgagct
    23521 aaacttttct gttcattacc tgtcccataa atataaattg tttctgcatc atatattatt
    23581 ttacaaatat cttgaaatga tattttttgt aattcattca ttaatagtcg ataattctcg
    23641 caaacatcat gaaaattatt atcattatta acaaattcta gttgattatt tttaagtaaa
    23701 tactttaaat cagagaatga atctaatcca atttttctac aaagccttaa taaagtagct
    23761 ctagaaacat tacattcatt tgcaattttt tcacacgaaa aattacggta ttctctttga
    23821 caaatcaata tttgtttaat aataatttta tcattttcat ttaaactatt ataactttgg
    23881 ttaactaacg attctaatct cattttttcc actccctaaa tattatttta atatacatta
    23941 gttgcttttt ctagtgaaga gtcttaaata ataaggataa aataatcatt tgattttatt
    24001 tttattaaaa taaaaataac ctgctaaaag caggcaccaa aaagtactta tcctttcgaa
    24061 catgtacttt agatattgct ttcttcggaa gcttcatctc ttcatactct aaaatagagt
    24121 tttttctaac tcaaaagtat tttccatggt agaaattact ttaattcatc tgccatttat
    24181 tttttatctt atttaataat tactattact tgaatattta cttctttaag taaatttcat
    24241 agttaacaaa gctaaaacta ttattataga tgttaaaata caaaattata agtattgtta
    24301 taaatatgct tacagctaca aatatacatg atatattaaa aagaagctgt aaattattat
    24361 catacataca agcaactatg cctctattaa tccatgttaa tttttattat ttaatacaag
    24421 gagttccatg agacatacat atccattcat attttaaatt atccaattct cttatagatt
    24481 tcatcatctt tttataatcc caattccatg gattaggata aggaactata tttccattct
    24541 tatttttgac taaatcacct gcaaataata catcttcaaa aatcatgcag acatgccctg
    24601 gagtatgacc aggagtatgg ataattttta tattcccaat cttcatattc tctccataag
    24661 gttcaacatc cttaatgagc cttctggaaa ttgcctttcc aataaacttc ttaaacccag
    24721 gtctatcctt ctctcctata ataaaaggta tatcctctac atgagcccat acctttgctc
    24781 ctgttttctc tttcaataat ttaatattag atatatgatc aacatcatga tgagttaata
    24841 aaatatgctt aatatctgta agtttaatgc caagtgtttt taattcttta attatatttt
    24901 tccccataaa aggaagacct gtgtctataa gaataatttc tttatcgaac acaataaaac
    24961 aatttgaacc acgagtacta tctaacatta atacattttc ttttatcttc ataaataaat
    25021 tccccttatt tggtatttta cctatataat taattaataa agttataaat agttataata
    25081 cttgatatta attttatttt ttattaaagt atactatagg tatacaaata ttatatttac
    25141 ataagtatac catcagtata cttttttatc aatagaaagg aataatttat atgtcagaat
    25201 tatcaaaaag agataaagaa aaaatacaaa gagaaaatga gattattgat aaggcagaaa
    25261 aattattttg tcttaatgga tttgattaaa aaatagctac gcacactcgt gactttagtc
    25321 atgagttagt agctaaaata gtcagcatat agggaaacct atatgtagag ataggataa
//
LOCUS       DAMKPG010000058        23638 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMKPG010000058 DAMKPG010000000
VERSION     DAMKPG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.061"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(486..1313)
                     /locus_tag="PC621_002356"
     CDS             complement(486..1313)
                     /locus_tag="PC621_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF4109505.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            1926..2381
                     /gene="smpB"
                     /locus_tag="PC621_002357"
     CDS             1926..2381
                     /gene="smpB"
                     /locus_tag="PC621_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF4109506.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            2539..2888
                     /gene="ssrA"
                     /locus_tag="PC621_002358"
     tmRNA           2539..2888
                     /gene="ssrA"
                     /locus_tag="PC621_002358"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3562..4467)
                     /locus_tag="PC621_002359"
     CDS             complement(3562..4467)
                     /locus_tag="PC621_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HDF4109507.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
     gene            5378..5593
                     /locus_tag="PC621_002360"
     CDS             5378..5593
                     /locus_tag="PC621_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF4109508.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
     gene            5593..6072
                     /locus_tag="PC621_002361"
     CDS             5593..6072
                     /locus_tag="PC621_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF4109509.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            6078..6308
                     /locus_tag="PC621_002362"
     CDS             6078..6308
                     /locus_tag="PC621_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109510.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(6424..7590)
                     /locus_tag="PC621_002363"
     CDS             complement(6424..7590)
                     /locus_tag="PC621_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF4109511.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(7580..8332)
                     /locus_tag="PC621_002364"
     CDS             complement(7580..8332)
                     /locus_tag="PC621_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF4109512.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(8424..8915)
                     /locus_tag="PC621_002365"
     CDS             complement(8424..8915)
                     /locus_tag="PC621_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109513.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(9166..9930)
                     /locus_tag="PC621_002366"
     CDS             complement(9166..9930)
                     /locus_tag="PC621_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109514.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            10077..10277
                     /locus_tag="PC621_002367"
     CDS             10077..10277
                     /locus_tag="PC621_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109515.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            10281..10595
                     /locus_tag="PC621_002368"
     CDS             10281..10595
                     /locus_tag="PC621_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109516.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            10617..11360
                     /locus_tag="PC621_002369"
     CDS             10617..11360
                     /locus_tag="PC621_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109517.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            11373..12707
                     /locus_tag="PC621_002370"
     CDS             11373..12707
                     /locus_tag="PC621_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4109518.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            12957..13229
                     /locus_tag="PC621_002371"
     CDS             12957..13229
                     /locus_tag="PC621_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109519.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            13502..14629
                     /locus_tag="PC621_002372"
     CDS             13502..14629
                     /locus_tag="PC621_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF4109520.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
     gene            15109..15756
                     /gene="cas5"
                     /locus_tag="PC621_002373"
     CDS             15109..15756
                     /gene="cas5"
                     /locus_tag="PC621_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF4109521.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            15747..16511
                     /gene="cas6"
                     /locus_tag="PC621_002374"
     CDS             15747..16511
                     /gene="cas6"
                     /locus_tag="PC621_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF4109522.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            16513..17679
                     /locus_tag="PC621_002375"
     CDS             16513..17679
                     /locus_tag="PC621_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109523.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            17700..18770
                     /locus_tag="PC621_002376"
     CDS             17700..18770
                     /locus_tag="PC621_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDF4109524.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            18781..20766
                     /locus_tag="PC621_002377"
     CDS             18781..20766
                     /locus_tag="PC621_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF4109525.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   21019..21641
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=21019..21047
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            22086..23638
                     /locus_tag="PC621_002378"
                     /pseudo
     CDS             22086..23638
                     /locus_tag="PC621_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706893.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         9430 a         2484 c         3605 g         8119 t
ORIGIN      
        1 agatataatt ttaaaaagat gtgtataaga acaaattcac ttaaattttt aaaatcaaat
       61 aaagttgaat ctcttgacta ccttgattgt tttatgaaat taatatagat gtaaactttt
      121 taatatagat gtaaacttta ctattaaaaa aatacaagat tttagataag ttaatgctaa
      181 ttataaataa taagataaga aatataatat gacataaggg ctaggaataa gataaatata
      241 atcaaagaat aagatatgag aataattacc aagagaaatc attaccttaa tatgtttata
      301 aatgttagac tatgttccta aattgaattt tatatatcaa gtataataac cttacagaat
      361 gtggaaattt ttatttaaag aatacacata aacatttcca cactctgatt ttataataat
      421 aatctaataa aatttatagt gactttataa aatcaataat gatttaataa atatttataa
      481 aacaactatt tttgcttaga aactttatta ttcatattat cattagtagg cattgctcca
      541 aaacttagtt tttcatacat tacttgagtt ggagctagcc aaactgtacc attaccagta
      601 aatgtattta agaaaccttc accatttaaa gcagaaccaa ttaaagattt atctgatttt
      661 gtaacagaaa aattaacccc agatgttctt gctattgcaa agttaccatc tacctttaat
      721 tcttctccat ttttcaattc atactctaca atttcacttt gaggtacaac acattccaga
      781 acgattacac cagtaccact taattctatt tggaagaagc cttcaccacc aagtaaagca
      841 gatgagaagt tcttttgtgt aaatgctttt acttctattg tatcagagca acaatagaat
      901 aatcctttat caacaattat agaatcatta tcaagactca taaataaata atgtctaaaa
      961 gaaggttcca aataaacttc tccagtacct ttgtatacag gtttcatcat tgattcatta
     1021 gtaagtgctc ctgatattcc ttttttaaaa agacctccaa caccaccaat cttagtttca
     1081 gaagatatat ttcctttata atagtataat gcaccagctt ctgtttttat tgatgaatta
     1141 tttaatttta tcttaatttg tctagctttc aaaccagctt ctctagcaaa atataatccc
     1201 attgccatta atgggctaga agctccctca agtttttcaa attctaatat ttcaaattcg
     1261 cttttgccat ctgatgcatt ttctaaaatt tttacttttt tgcttaaatc catacaaatt
     1321 tctcccaact atgataaaat ttatctctaa aatattataa gatataactc tatattacat
     1381 tttttttgaa aaaatcgcta ctttatcatc aaatttttac atatttttaa gaagtttttg
     1441 aaatgtttag atttaagtta agttatttaa ggatgtatag ttatttcaat agatattttg
     1501 agtattattt atcatttgtg ggtattatat tttatgggca tgttaaaatt atttagaaga
     1561 tagaagatag aagatagaag atagaagata gaagatagaa gatagaagat agaagataga
     1621 agatagaaga tagaagatag aaaatttata atatgtaata tttttgtttt gaaatttaga
     1681 aatcagtgtt actggttaaa gttagtttag tattaagtat attcgaagta gttttttagc
     1741 tattttcata tctttatatt acttaattat atatagccta actaacttaa tatatcagtg
     1801 aaatattgac taaaatgcct aaatatgtta tcatacaagt tagtcaaata atttggcaac
     1861 attaaatatt tagtaaattt ctaacattta tggaataaat ttctaacaaa gaaggtgaga
     1921 tttatatggc tggtaaaaaa atattagcaa caaacaaaag agcaagacat gaatatttta
     1981 tagaagaaac ttatgaatgt ggaattgaat taaaaggaac agaagtaaaa tcaataagac
     2041 aaggcaaagt taacctaagt gatggatttg catctgtaga taatagtgaa gtatttttaa
     2101 aacaagttca tataagtcca tatgagcaag gaaatatatt caatgttgat tctttaagag
     2161 ttagaaaact attacttcat aagcatgaaa taagaaaact tattggagca actactataa
     2221 aggggtattc acttatacca cttagtttgt atctaaaaaa cggtaaagtt aaagtagaga
     2281 ttgccttagc aaaaggtaaa aaactatatg ataaacgtca agatatggct aagaaagatg
     2341 cacaaagacg tatagaaaga gaaatgacag gtaagtatta aactagataa aactgtattt
     2401 tttagaataa aattctttat tatctagaac ataacattga tttttattct taaaagtatt
     2461 atattatata tacattaaaa atttaataaa aatggtgata aagtttgcga aaagtctact
     2521 ggactataaa ctactcaagg gggcgtaaag gtttcgacgt gggtttggaa cttgaggctg
     2581 catgtcgtgt tactctgggt cacgtaaaaa ctggggaact ttaaaataaa cgcagacgat
     2641 aatttcgcga tagcagccta gttgctgctc gtccctctta gtcctcctgc cgactaagac
     2701 tggacgtcat tatgcaggga actactttag gggtgtctcg acctgaagta gattaattgg
     2761 gactggctaa gcatagagcc tgccacttgg cgctatgtga ggtgagatat tagtgaagtt
     2821 ggctaagcat gtagatgcag caaggaaaag atttgcggac aggggttcga ctcccctcgt
     2881 ctccaccaat ttttaaaaaa gaatgatgaa atacttggaa atactagtct ttattgacta
     2941 gtgttttttg ttttggagat gaaatccacc aaccgtgccc caaccacaga aaaagagatt
     3001 tttttggggt atattggggt gcatttgatt aatccatgga acatataaag aatactttta
     3061 gagatatagt gaatttgatg ataatacaat aaatatttta tactgatgaa ttgtttttat
     3121 attatgcttt ttatttcaat ctaggaaata tataacttgt ttaaacttat gattaaaagg
     3181 ataaagaagt ttaaagcata taagtgctac ttaatctatt agagtaatta ctagttataa
     3241 tgatatacag aatcctataa gattatgtat aagtatagaa caaatttatt atctatgtgg
     3301 gatgctaatt taaatttttt aattgtattt aaaaaaataa gatgtaaaaa aggaattgaa
     3361 aaatgttaag ataataaaca catagaatta ttaagtttaa tactaacaag actattgtat
     3421 atagacaata tattatatat aaaatttaaa taatatatca taaaagtacc tatactaata
     3481 agtactttta tgatataagt taattctatt tttataatat aaattaaaag tatttaaagc
     3541 gttgttaaag gctatagtct attattcatc atattcgatt tcgagctgta caggtagatt
     3601 gaaagctcta ttaaaaagca cattgaaatc tttccagtct tttgatgctt gcattaaggt
     3661 tataaccttt aataaatgat tttttaaatg atcattacct atatcttctg ttaaaaattg
     3721 atgatgttta tgttttcttt gcccaggttt tactgtgggg ttaactcttt gaagttcttc
     3781 tttaactcca ggaggtaata aatcataaac atatttattt gtgtatgttc caactattcc
     3841 aggtctctta gaacttggtc tagggtatgt ccagtttttt aatctgaaca tttgtctata
     3901 aaattcatca ggaaatcttt ttgtccaagg taaaaactct ttagaaatat acatagacaa
     3961 tattttttgc agctcatcac gatctctatc atattgatat cctgtagctt catcaactaa
     4021 tgctacaatc cctattttgg ataaacttct tactaaaatt tcgcttgcta tagctagtcc
     4081 ttcttgctga acagctaatt tattatcaag ccttgcttgt aaatatacat cacatataag
     4141 aggtattatt tcagctggat aaccttccac aattcttccg tttttagtct tatattttat
     4201 tgtatttata agcatatctt ctaattcatc tgaaatataa tcagatagat tctttgcatc
     4261 aataaaactt ggtagattag gcttagaagg tacacgaatt tcatcttttt ttctaccacg
     4321 ctttgttctt ccaaaagctt tgaatactgt acttttattt atgattcttg aaccattttc
     4381 tagtacagca caatttaaat ctttttcacc tatttttata ataccaaaat gtgttgctct
     4441 caatatactt ttttcttttg attccaattt aacaccatcc tttattttat tttaataagt
     4501 aagatattac ttagtatctc ttcattagat aaattagtat ttataagact aataatatca
     4561 attttaaaat atgtatatag ataattgagt ttttttatca tcttgtacgc agtattttta
     4621 ttaacaacga tattttttga taattttcta tagctaatat tttcatcaaa tataaataaa
     4681 ttaatagcaa gtagccattt tctgtagtct agtttagttt tatgcataat ggtattagtt
     4741 gtaattgaaa acttactatt gcaaatattg caatgaaata aatcattata agtactaatt
     4801 ttatcagaat aacaataggg gcatattaat gagtttttta atttaagatt atataaaaaa
     4861 atcttataag agacatctgt tggaaagttt ttataaatat caaagtcttt tgtcataatt
     4921 taattatatc atgttgtaga aattaattta ctgtatttaa gtgggtcaaa tttaatatat
     4981 atgtttttga tagtgtaaaa attaaaatat aattttaggc atgtttgtat tgaataatga
     5041 gcactttcaa attttattag tgtaagaggt ttcaaattat aaagtaatga atgtatatta
     5101 ctatatgatt tataattaga ataaaatttt tataatagaa atttaatata tcaaaatatc
     5161 agttgaggat attgttcaag tggtatatct tccctgagtt aaaaaataaa tgatgaaata
     5221 tttggaaatg ctagtcttta tttgtttatg atattatgta acatagaatt ttaatatcta
     5281 ctttatgata taatattttt ataaacataa gatggtttaa gttattaatt aataaaaata
     5341 tatatattat ataagtggat aggagttgag gtttgtaatg gatgaaagaa aactagaatt
     5401 ttctattttt tgcattgaag ctttagcaga aaagttgaat atagattcag aaggagttta
     5461 taaacttctt aaacatgata ctgatatttt agataactat ataatacctt gctatgaagc
     5521 actacattct caaagtaaaa gttatatagt ggaagattta atacaagtgc ttgaagaaaa
     5581 gggcgtgatg taataattgt atatcatgat tcatactgtg tggcaaaaac gcctaatatt
     5641 atcttcttaa gagattcttt agattttgga aaaggattct atgtaaccag tataaaaaca
     5701 caggcaatta actggataga acgttttaag aaaaggggta aaaaagcata tttaaatagc
     5761 tattctctag atattgataa attaaaagaa aactgcaagg taaaaatctt caatagttat
     5821 aatttagaat ggcttgactt tatattagaa tgtagaaata tgagtgatgt atatatgaaa
     5881 tatgatgtta taataggtgg aattgctgat gataagattt acaatacaat agaactgtat
     5941 caagataatc ttattgaaaa agatgaagct ttaaaaagat taaaatacta taagccaaat
     6001 gaacaaatat gtataactaa tcaaaatgta atagataaat atttaaagtt tagatgtgaa
     6061 gaggaggtat agtgcttatg gaagcaaata aaacattatt acagatacta tataaaaaaa
     6121 ttatattaga attttctaat cagacaggca agagtttgga agaaagtatg gattatttat
     6181 atacttctga aacatataaa cttataagtg agggtgtatc agatatgcac tgtaagggtc
     6241 atatatactt ggctcaagaa ttaatgctag aaaatggatt aatgtatcat aaaggctatc
     6301 caagataaat agttaaaact agatacgaat taaggataca agataatata aaattaattt
     6361 gtatccttat ttgggttttg taattaacta tatgaaatca tagtattttt atgtatttga
     6421 tttttacaat gaaaatattt tatccaactt ttctacagct tcaaccttct tatcttccat
     6481 aacttgagta tatatattta aagttattga tatatcataa tgacccatta aaacttgtac
     6541 agttttagga ggaacatcat tctcaaaaag tctagttgca taagtttttc taagaccatg
     6601 aaactttata ggttctatat ctaagctgct taatatagat tttaaattcc ttaaaggtct
     6661 tttatcatct atagggacac ctaattcatt gcaaaataca taattatcat ttctatataa
     6721 ttctccagcc tttaatatat ccacattttg atttacttta tgctctttca atttatttaa
     6781 aacatctttt ggaataggta cagtcctgta agaatttgat gtctttggtt cttgctctaa
     6841 tactttaagc tctctatttc catttttatc tataaaatat gttctttgaa gggttctttt
     6901 aactgttaaa ttagaatttt taaaatcaat atcaaaccat ttaagtccta ataattctcc
     6961 taatctaagt ccagtaccaa gagcaaccaa aaataatact tctaatttat gcccctctat
     7021 agcttttata aaattctttt gttgttcaag agtcaatatt tcaagcctat tttccttttt
     7081 ctcaactggt atatttacca tagtacaata attacgttgt ataagacctt gtttttcagc
     7141 ttctgataag caggttttca gtttagtgtt tatttgcctt atagtaggta tacttttatt
     7201 ggaatcaagc aatctattgt aatatttttg catgtgagat actcttaagt cctttaactt
     7261 aacattgcct aaatcagtat ctttaatata attacgatag atgctttcat atctttcaaa
     7321 tgactttggt tttaaatctt ttattctata ttcaaacaac caattgtaaa accaatgctc
     7381 taaagttatt ttatcatctg aaatatcaga acttaaataa tattgttttt tataagcatc
     7441 aagtttatct ttaacttcct tttgagtttt accataaaat tcttttctta ttggttttcc
     7501 atcttcatta taaccaatca ttatacgaga acgccaacca tctttatatt ttccgataga
     7561 gccttctcca ttagctcttt tagttgccat ttttaaacaa ctccttatat gatttatagt
     7621 tatatccatc gaagaatgta atacatttct ctaataagaa ttttataact tcatcagctt
     7681 ctagtgtttc atagaagttt agcattaatg tgttgtattc ttgtttatgt ttggctggta
     7741 ttgatagaac tcctatatca ttacttataa gtattaggtt tgaggtaagt cgagcagttc
     7801 ttttgttacc atcatagaag aattgacaat aggacaacca taaatttatt attatagcct
     7861 tttctaaggg gttagttatt ttatttatga tacttatgtc acttataaat aaattattta
     7921 atagagtggc ttctatgcac tcatattgag tagtaccagc tatacctatg ttaccgtttc
     7981 taaaatctcc aacaattaga gcttcatctt ttgcaactat tttatgtata gagcaaattg
     8041 tatctttatt taaagataag ttttctttgt tacttaattt tattatataa tcccatgatg
     8101 attttatatt tagtatttga ttttggtcac ttaatttatg tccacctact gttataccat
     8161 caagtaatgt ttgtacttct gggaatgtga aggggttatt ttctaggtta cccatatcat
     8221 atacaaattc acttatcatt cttctaatgt atgttaaagt atacttttta tctccatgag
     8281 gtagattcgt agggaattta tgtgtgtatt ttggtatata tgatttattc atgattttag
     8341 ctcctttcat atctaatttt tatttattaa ctatgaatgt ataaaagtta ataggtaaac
     8401 ccatattttg aaaatgtgca atactaatat tcttcatcct catcttcaat aagaccatta
     8461 gagttttctt gtataaaatt ttcaacaaga ccatttatca cccaatttag atttttggag
     8521 aagtctttga agtcagagaa gcttaagcta attggtttta ttgtatctgc attatctgta
     8581 atttcaaact tttttttata gtctgttttt atatcataac cataattttc taatatgtga
     8641 actactaaat ccaactcttt atcaaatact tttaaattaa tattattatt tgttaaagta
     8701 tctaaatcat tttcttcaac aatgagttta tttatggcaa ttgataaagc agtagatatt
     8761 tttcttatca tatctagact aggtattctt tgattttgct catatttagc tatagcatga
     8821 actgaacaac caatcttgtt ggctagctca ttccttgaca tatttatcga tttcctatat
     8881 tgtttaatct tttctccgat tgtattattt atcataattc caccttaaat atatcatcaa
     8941 acaatgattg tatttttaca catataaagg agttattttc tagattatct atatcatata
     9001 ctaacttact cataattctt ttaatgtatg ttagagtata ttttttatct ccataaaata
     9061 aattagtagg gtatttatat gtgtatttta gcatatatgc tttattaata attgtaactc
     9121 catttgtagt tttttatcat tgctttagat agtattgata ttattttata ttttagttag
     9181 tagttttttt atttctttaa gtgtattttc aagcttttta tttttttcct ttaattcatt
     9241 attttcttgt aataatttaa agtaatcatt atttttaata gtcatatttt ctttttgaga
     9301 tattaacttt tttagtcgaa tgttactccc attatctgtt gcctcactaa agtaatcata
     9361 gattgcttct atattttctt ctgatagacc aatatctaat aattcatcaa ttgattgttt
     9421 tataagaaca tcatttttaa taatttcttt tatatcatca ggatgtaatg aaaaatctaa
     9481 ttcagtatta gatacacaat ctgaaagtat ccaattataa acttgatttt catttagttc
     9541 taatttttca ccaagtttat aatattggtc tttatcagac caagcaccta gcaataaact
     9601 tgaggtaatc ctaatatcat catcttccat aagatttgaa atagcattta aaaattcatc
     9661 aaaagaatag tattcttctg ggaataaaga ctgtaaaaaa tataaaacat cttcattagt
     9721 cattacttca ttttcaatat caaaataaaa gatagtcttt tcaattttta aagctttagc
     9781 gagttttgat aaggtatcta tactaggtat tcttttatca ttttcataat ttattagagc
     9841 atttcttgag atgccactaa tttcagcaag ttgaatctgt gttagttctt tttgttttct
     9901 tatctttttt aagttttttc caatttgcaa tattatcacc tcgtataaag tatatcccaa
     9961 aagtacacaa atgagaacag aaaaataata tattcttatt gacagcacac atatgtcgac
    10021 attataatta aaatgtaaag tttacgaatg taaactagaa aaaaggagga aaaagtatgg
    10081 gaatcaattt aaaaatattt aggattaaaa aaggtttaaa gcaacaagaa ctggcagata
    10141 gagttggtat atcaagatat tatttgtcaa acttggaaac aggtaaggct aataatccaa
    10201 gcaatgattt gatgattaaa ctatctagag ctttagatac aacagtagaa gaattatttt
    10261 ttagtaagga ggagtaaaat atgaaagaga ataataattt agaagtttta aagacattac
    10321 aagctacttt aaacattatt gtaaagaatg aagaagaagc acaagaaaaa aagcaaggat
    10381 ataaaattat tgaaaatgcg aaaagaaata aagctgaact tataacatgt aaagaagcat
    10441 ctaagcttta tcctatagga gaatcaaaat ttaggcagtt atgtcactct aaaaataaag
    10501 gtttcccttg tatatggata aataatcgag tatacataat aaaaaacaaa ttggatgatt
    10561 ggtttattga aaatgcaaat ggtatcaagt tatagtctta aagtaggtgt ttgcaattga
    10621 gcaataaagg gtggataaag ctttacaggg agttgttgga taaatcaatt tggaaagata
    10681 ttaaaccaga aagaaaagta atattaataa ctctattact tatggctagt cataaagaaa
    10741 atcatgtttt tatagataat aaaaggataa aaataaagtc tggacaattt atttcttctt
    10801 atagcaaaat agctgaaaga tgtggtaaag gagttagtta tcaaatggtt agaagttgtt
    10861 tgttggtttt taaaaatgct ggttttctaa catacgaaac aacaaacaaa tatatagtca
    10921 taactattga aaactgggag ctttaccaag agaacataga aaatcaacaa cctaatcaac
    10981 agacgattaa caatcaatca acaacaatca agaatataaa gaatgaaaat aatattatat
    11041 atagtgaaat tataagcaaa tttaactcaa cttgtattga actttcaaag gtcaagaaac
    11101 taacagacac aagaaagagg agaataaatt caataataag agaatatgat aaagaagctg
    11161 tattagaggt tataaatata gttagtaaaa caagttttct gaatggaaat aataatacag
    11221 gatggaaagc aaattttgat tggataatga attcaagtaa ttttaccaag atactagagg
    11281 gcaattatca tagcaaggaa attgtcataa ataaagagac aggaatatat aatccagatg
    11341 attcacaatt aaagtggtaa ggaggttata ttataaataa ttcaaacaac ttaattaaaa
    11401 atacagaagc agagcaatca atattaggaa gcattttatt agactcaaca atagtagaag
    11461 agttagagca aatgttaaat gaagacatgt tttatcatga ggaacataaa tacatattta
    11521 atgctataaa agaaataaat aagagaaatg aagtaataga tataataact cttacagaag
    11581 aattaaagag aaaaggtcat ttgaataata ttggaggtat aagctatatt accagtttgt
    11641 caacaatagt cccaacagta tcaaatttca cgagttatgt cagtatagtt attgaaaaat
    11701 ataaaaatag aaatataatt gataaattca ataagttcaa tcttggagaa atagatgata
    11761 atactcttat atatcagtta gaaaatgata taaataaagc caatcagaaa ttaataaaaa
    11821 aggattcaag tataacagcc atatcagaga gagcctttga atatataaat gatgaaatga
    11881 atgaaggtat taagactgga ataaagttct tagatgatac tataggaggt ttatatggag
    11941 gtgaactaac tacaatagca gccaaaagtg gaagggggaa aacagcatta gctttacaaa
    12001 tacttagaaa tgtaatattt caaggaaaga aagtactttt tataagtgga gaaatgagtg
    12061 atatacagat tctgtttaga aatatagcct cactaactgg aatatcagtt gtaaaaatga
    12121 aaaatagaaa tctaaatgat gatgaaatag tcaaatatat acaagcttta agcataataa
    12181 atcaagaaaa taacctttat ataaatgact ctgtaaatac agtatcaggg ataaagagag
    12241 aaataaagag tactaagcct agcttagtca taattgatta tttgcaaatt ttggattatg
    12301 aaggtaaaga agtaggagaa gctaaatttg ctgacttatc aaggcaaata aaaaagatta
    12361 ctttagattt taatatacca gttattcagc ttgctcaatt aaatgatgaa ttgaaggatg
    12421 aaagacctaa aggggataga gtaatgagaa gctcaaagca gatatatatg gattctaatt
    12481 cagttatata tattcataga cctacagaaa aagaggctct taattattgc aagacaatgg
    12541 gaattgagga tataagagag ttgagacgaa gagaaaaagg ttttggtgct aatttaagtg
    12601 agattattgt tgccaaaaat agagatggtg agtcaggatt tagaccatat tggtatatag
    12661 gaaatttgtt ttcttttgaa gaatgtaaga agatacaaga gatataaaaa agaagcatga
    12721 tttattaagt aatagataaa ttatgtttca agaagtattg aatggtaaat atataaaact
    12781 taattagatg taatagagat ttaaaatata ttgagtttag atttaaatgc ttattagaaa
    12841 aaggtagttg ccatataaat ataacaacta cctttataaa ttaggtactt aatgtacact
    12901 aacctagcaa cttaagtata tattaagtac ctgatgaaag tcaaggaggt actaaaatgt
    12961 atgatgaaat tatagaaata tattttgatt attggataaa tgaacatatg tatagaagta
    13021 ttttagaaaa agatgagtat tatattgaat gttggaaaga ggttaaaaaa attaatgagt
    13081 tattggaaat aaatttggaa gatgataaga aatatctgtt ggttgaatta aatgatttgt
    13141 ttaattcatt aatagaattt gaaagagaac ttagttataa attaggattt aaaacaggag
    13201 caaaatttat aatagagttg ttgaagtaat taatttgtgt atctcaatag aataattaaa
    13261 tagtaaatcg agattattaa taagttgtac tagaatattt taagtgaagt atatattaat
    13321 tatattgttt aaaataaata tatttaataa agaggggata agtatgccct cttttatttt
    13381 tatgcaatca atgacattgt aggatttacg tattttgata aatagcacaa tatattttaa
    13441 taataaatat atgatacaat aattttatta aaatctaaaa taatcaattg aggaggagaa
    13501 tatgataata cctaagttag agcctaatga tagaaaaaga gagtttaata aatactcatc
    13561 agagcaaata aacaaagtag tttataatta tttatttaaa aataatatga gtaatagaaa
    13621 gatagatgat gagatactag atttatcatc aaaacaaagt aaaggttatc aatcaatggg
    13681 tatagtgcat catttaggaa taaataaaaa tcataaaggt atttttacag gatacacaat
    13741 agataaagca attgaagttc ttaaggaaga tgagatacat ttcaagcaga ttattaatat
    13801 acttttagaa attagaaata taaatgagta tcctataata gttgactctt gggagttatt
    13861 agatgagaaa atagctttaa aaacaattga taaatctaca ttaaaacata atgggacagg
    13921 agttccaata gaaataagat atttttttga gataaataat ttaggaaaga atgaaaagaa
    13981 aaatgtgaat tttaaatata atgataatta ttatagtgca gttatagaga tggataaatt
    14041 agagaatcca agaagtagaa taatttggaa atctaagttt agagatttac taagaagtat
    14101 attttctgag tattatgaga aattcttaga aaataaaata gaaggaaaaa caaatttacc
    14161 taaaataaga ttcataaaac ttgatagtaa taattttaac atagaattca tttttactgc
    14221 tgaaatagaa gacgatgtat tagaggagat aagtcaagat aatcctttga acattaatac
    14281 tattaatata gaattagaaa ctgaaattag aaaagaaggg aaagttaaat atgtttatgg
    14341 gaaacagtat gagcgtaacc ccagaaatag aatagaagca ataaaatatc atggcacaaa
    14401 gtgtattgta tgtgggtttg acttcgaaaa aacgtatgga gatagaggta agggatacat
    14461 agaaattcat catattaaac cattaagttc tgttggagaa gaaagcaata taaatcccaa
    14521 aacagatttg gtgccaatat gttcaaattg tcatagaatg attcacagga agaaagataa
    14581 tgttttgagt attgatgatt tgaagcaaat tataaaagat agtaaataat gcttatgtgt
    14641 ggcagagatg tataaagtat atattctaaa ctagttaaca acaaatacta atataaaaaa
    14701 tcaaaaaatt tattaggaaa aggtagttgc taaagtataa cagctacttt tataaattaa
    14761 gtatttgagg tatattagtc taaccgatta ggtatatctt aagttattga tgaaaattaa
    14821 ttaagactaa catgtatgat gaatccatag aaatgtattt ttattgttaa ataaatgaac
    14881 atacatagaa atattttaga aaattacaag tactatattg aaagtttgaa ggagaacctt
    14941 gatttaaggt aagtgtaaag tttttagaaa cttcactaaa ataatctaat taagttatat
    15001 atttgttact tttaggaaaa taatggtaaa aattaaaggt ttttataaat gaatgtctaa
    15061 ttttataaat aaaatggagt attttaacta aatatttgag gagataaaat gatattaaat
    15121 aaaatcgaaa taattggaga tatggctcac tttaaaattc ctatgcatag caagatacaa
    15181 agaacatatg aaattccacc aatatcaaca gttgtcggaa ttttaaaaaa tatatatggt
    15241 gaagaaatag atgattttat attaggatat gttatatatc atgatggtac atttaaagat
    15301 ttacagacta tatataaaga aatcaatcca aatgtaaaaa cattgacaga tagcgataga
    15361 tttcaaactg atgtatgtcc cattgaatac ctaattaatc ctaaacttat catatatact
    15421 aacatagata aaaatataga atttaatgag ccgctatgtt taggtaagac taattgttta
    15481 gcgaaggtat tatctttaag tgaaattgaa gtaaatctaa tagataaaaa agctattgga
    15541 tacaatcaat atacagatat taatattgga gatggaatga taaaaagaat aaatacattg
    15601 acagagtata atgaaaaaaa aggttattat gatatatatt cttcaatggt aagagaaaat
    15661 gatgagtttg aatatggtaa atattatgat gaagatgagc aacagaatat ttttttatgg
    15721 aaatggaaaa aagggggagt aattaaatgc atttagtaag aagacatttg atactcacag
    15781 tggataatga agttgtatta gattacaatt accagtatga gctaatgaaa aggatatatg
    15841 aagctataga aattaatgat aaaagaaaag cactaagtct tcacaatgaa ggctataagg
    15901 tggataagaa agtatttaaa ttatttaatt acacaattat gtttgaaaat gctaagtatt
    15961 taaaagaggg aatacattta aatccccaaa caaaaatcaa attaatttta agtggatatg
    16021 atgatatatt gaataatatt ataaaaggtt ttattaaatg taaagtattt aaattgaata
    16081 atatagagtt taaagtttca gatatagaag aagattcaaa gaaaaatttt aataatataa
    16141 cactttataa agtaaggtca cctattgtag caagtttata tgatttaaaa agtaggaaac
    16201 aagtatactt aaatccaatg caagaagagt tttataaagc attacatgat aatttaggaa
    16261 ataagtataa gcttatacat aataaagaat atactggtga actatacttt gatattgagg
    16321 atgtattagc agtaaaaaag aaatatataa ctaatataaa aggaaaagga tttataattg
    16381 gatatacaga ttttgaaata tttgtacaag ctaataaaga tatgcaagaa gtaatttatt
    16441 attgtggatt aggtgaaaaa aatagtatag gaatggggtt actaacttat ataacttcta
    16501 ggagggctta atatggatag aagttacaat gtatttgttg ataatggtct atatgtgttg
    16561 gcttattatc tgaacaaaga tattaatgat ataacttatc aagatataga gaatagtata
    16621 gatttaatga gtgataaaat tgaagaattt gtaagttgcg aaaaatactc taatttaaaa
    16681 agtatgtgct ttagcaattc tacgctaact caaccaaaag gaaaagttac tttaaatgag
    16741 aagttaaaag gttttgttaa aaatcaagga gatgaatatt gtagtttatg tggtcaatat
    16801 aaggccaaag tcaaaattga agataaagaa tacaatatag ggagaagtta tatgcctaat
    16861 ttggttgcaa atacatttta taatttctcc aataatttgc aaggtttgaa tgtatgtcca
    16921 tattgtttag tattgactat gtattcaatt ctgaattgta gagtaagtag gtatgctttt
    16981 ttatacaatt ctacatcaag cgaatttatg gaagattata ctttccatat tcaaaaagaa
    17041 aatttaactg atgtagagtt aggagcaaag aaagaaaaag aaaagtattc aattgtaaaa
    17101 ttattagaaa gtctagtgtg taaatataac ttatttaatg gtgatataga gcaatatatg
    17161 tttaataact ctggtcaatc tcaggatatt caggtaaata gaataaaaaa taaatatgtg
    17221 aatttattaa taaaattaca ggaaaaagca ctactatcaa actttgaaaa gttacatttg
    17281 gataggtata tcttgaatgg tactttagaa agcaattatc tcagagaagt atatagggta
    17341 aaaaaggaag aaaaaatgga tgagaaagaa caaaagaatt ttgatgaact tgttattttt
    17401 ttaaacaggg aggtaaatgt attgaaaaaa gtcaaaatag atataataaa aaaattaacg
    17461 gataaattaa aaactagtga cttagatata gacaaaatat ataaggagct taaaatgtta
    17521 ggaaaatttt atgcatatga ggactggtta gttaacttaa atgaaatgta ttatgaaaaa
    17581 actggagaaa aactatttga agcagaggat tatattgagt tggatgatat aagaagcttt
    17641 agacagatta aaaatttaat attagtaagt ttaatttaat aatattatgg agggttaaaa
    17701 tggaaaataa aaattcaaat agaatgttaa atataggcta tgcaatagaa gttttatgtg
    17761 ggcgattaaa tggaagtagt agtgaagtag aaaagaaaaa taattcatat aagaattcta
    17821 cttatactaa aaaaataggg agcagaagta gaatctcaag tcaatgtcaa aaatacaatc
    17881 aaaagctatt tatggaacaa gtttgtggtt atgaacaaaa tgttaaaact aaagatggga
    17941 aacaagtaaa attggttcca aacccattta aatatgcgac agatgatata tttgggttta
    18001 tgatggcgga aaaattatct atagaagaag atgtatacaa agaattatca gatgaagaaa
    18061 aagaaatata taaaaaaaat aaaaagaaat atgaagtaaa tataactaaa aaaagaaagt
    18121 caagatggca aatgaactct ttaataaatg tatcaaatag aaaagttgaa tgggagtgga
    18181 atgtttgtgg aactacagga gatagtatgc catatcaagt tgaggtatat tcaggggtac
    18241 atacaaatat agcaaatctt aatataggaa agataggaga atatgatata tctgatatag
    18301 caagtgagca tagagattat acaactttag aagctaaatc aattggagtt gaaaagttag
    18361 ataaagaaga aaagtttaaa agaatagaag ctctcctaaa atcactagaa tacttaagtg
    18421 tagaaggtaa taagacaaat catctaactg atacaaaacc caaatttgtt atattgggag
    18481 agtattcatg gggaaataat gtatttcagg gtattatcaa agaaaatggc ttagatgtag
    18541 agatgttaaa agaatctata gaacaaaatg aagaatttag aaaaagcgat atatggattg
    18601 gaataaataa attttatgat gatacattta atgagataga agttaataat gaaaaggaac
    18661 aaataaagaa gtatttagaa gaagagtttg aagagtatga ttttatgaaa atatcaaatg
    18721 tacatcaagc ttttgaaagt tacttaaaat atttaaagga gactttataa aggatatatc
    18781 atgaataatt tatatgaata taaagctaaa tcagatggga caagtataga taagcataat
    18841 gaagatatga attacataat gaatcaatta gttagcatat ataatatatc taatgatatg
    18901 aaaaatgatt tatatgaggc aattgtatgt catgatgtag ggaaagtagt aaaaagcttt
    18961 caagataata ttgaaagtag cacaagaaga gttagacatg agttattatc agcaagtatt
    19021 aagtatttaa atagaggtca gaagttagct atattaaccc accataaaga tataaagttt
    19081 ctagagggtc aaataacaaa ttctatttat gaaattgaat taaaggaatt aaaggaacta
    19141 ttagatattg aagtagaaga tataagacca tttataaagg aaatatcaag agcaagaaat
    19201 gatgatacta gaaaacttga aaatatatta ttaaaaggtt acttgcaata ttgtgaccat
    19261 atagcttctg cgggaattaa aagtatagat aaaggattta attctattaa agattttagt
    19321 ataccaaagg gtgtaagtta taattcaata caagaaaaag ttttaaatat ggataaaaat
    19381 gaagatataa tgataatggc tatgacaggt cttggaaaaa cagcaacatc attgtattgg
    19441 agtaacctag ttcaaaatga atataaaagt aagagagtgt attatatact tccttataca
    19501 gcaagcataa attctttata tgaaaattta tataatagaa atataagcgt aagtatgtta
    19561 cacagtaaag ctccatattt tttgaataaa attagtgatg aagaagatat aaaacaactt
    19621 taccagatat acaaaaagag tgtaaaacaa attaatgtat gtactatatt tcaattagta
    19681 aagtcagtat tttcttgtaa gagatttgaa atgctactat cacaattaaa agatagtata
    19741 tttatagttg atgaaataca ttgttttgat acaagagtat tgtgttatac attggaattt
    19801 ttaaaatatt taaaagaaaa cttaaatgta aaaatatgta taatgagtgc aagtatacca
    19861 acttgcttaa aaaagttaat acaggaaaga ttgcaaataa gtacaattat tgaaccagat
    19921 agtaaagatt taatatttag acatagattg aatagattaa gtgaaggaat acttgatagc
    19981 atacataaaa tagaaaatga tttaatgcaa ggaaaaagag tattaatatg tgttaattca
    20041 gtagatttat ctcaagagtt atatggaagg cttcaaaaat ataatcctaa attaatacat
    20101 ggtcgattta atactagaga tagagagcag gcagaaaaag ggatagaaga aagcttctta
    20161 ttgataggta ctcaaagtat agaggtaagt ttagatataa gttatgatgt tctttataca
    20221 gagttagctc ctttagattc attgttacaa aggtttggta gagtaaatag aaggggagat
    20281 aatgctgagt tgagcaatat ttatatatat gattatgata gaaagcttaa tatatatgat
    20341 gaagatataa taaataggac tgatatagta atatcagata taatacataa tgataattct
    20401 atagttgatg agagtaaagt tccatcttat ttagataatg tctatttaga tatagatatg
    20461 gataaatata ataaacataa agttgcttta gagattataa tgaaaaatat gaggttaggg
    20521 gtatggaata aatctgctaa taatgatatg ttaaataatg atactattag tgttgtgcct
    20581 gaatgtcttt tagaagagta caagttgtat attgataaaa aaatgtattt agaagcgagt
    20641 tcattatttg taaatgtatc tgtaaaaaga tacaattata ataaaaagat gttttatgaa
    20701 tgtggagata tagttgttac aagttatagt tataatgata aactaggtct aatatttgat
    20761 tgttaagaga attatagtat aactaattaa aatattttat tgagaatttg ttataattta
    20821 tatcgactac aaattttata tgttacaata gaataatttt gcagtgagcg aaaaatatgt
    20881 gtaaatggct gtgagttagt gatggcaagg tttataactg aatttttgtt tttagctaaa
    20941 aacactactg gtgcctcact gcaaattaac tattttaata accctacaag tgttgtaatt
    21001 actatagttt tatttggggt ttaaattaca ctaagttagt tataaatatt ccggaaagta
    21061 cttttgctat ttgattcatt ctacccgttt aaattacact aagttagtta taaatttcac
    21121 taattttagt tacaactatt acaagtccag ccgtttaaat tacactaagt tagttataaa
    21181 ttatgtgtaa tgtattttaa tgttttctag caacaatgtt taaattacac taaattagtt
    21241 ataaatttta taaattctat gaataatttt aaagagaggt ttcgtttaaa ttacactaag
    21301 ttagttataa attgtacatt tgacatagaa gcttcaaaat ccatcccagt ttaaattaca
    21361 ctaagttagt tataaatgta acagcacgaa tgatggcaca gacctatctg caatgtttaa
    21421 attacactaa gttagttata aatagaagta cacttacaag taagataaaa gacccaggta
    21481 cagtttaaat tacactaagt tagttataaa ttctgtagct atatgtggaa gtaattttag
    21541 tagagaggtt taaattacac taagttagtt ataaattatt gaaggggtga gagttgtgac
    21601 taggcactac aagtttagat tacactaaat tagttgtaaa tttacttatt cattaagaaa
    21661 cttaactgag agtttgattt atattattga attagcaaga gttttagaaa gtagaaaatt
    21721 ataccccaat aataccccaa ccacaaaaat aaataataaa aaatatccaa aaaaattagt
    21781 ataacatcat aactaaatag ctatatacct acacttacga aaaccaacaa aacaatcaaa
    21841 taaccacttg tagactcccc tcgtctccac caattttgaa aatgaaaata atagaataaa
    21901 aggaattact tatgtttgga ctaagtaatt ccttttattt tgcttaaaag cacacaatta
    21961 gcacacaact agaaggttat ataaataaaa aggctgtcgc actagaaaat agtgctacgc
    22021 tcttttttgt ataaaaaaat aaatcccaag ttcaaaagaa cctgggattc ttgtataata
    22081 actgtatgct aaaacaaaat actacacaaa ttgattatac tacttatggt gataattatc
    22141 aatttaagct accactaaat attgattaca tgattccaaa agatgattct gtaaggttat
    22201 taagttaaat tattgaggag atgaatttag aaaaattata taagacttat tctcgaatta
    22261 gatgaaatag agtaatccat agacaatttt tgaaagttat aatatataca gatatgaact
    22321 gtatttattc ttcgagagaa atagaaagaa catgcaaaag agatattaat tttatgtacc
    22381 ttctaggcga aacctccact ccagaccatt caacaattgc aaaatttcgc agtatacatt
    22441 tttaactaat aggcaaaagc ttgattgcac agtttacaaa ctttcttgga gataataatg
    22501 aaatttcaaa agacacaata tttattgatg gtacaaaaat agagtcatct gctaatagat
    22561 atacttttgt ttggaaaaaa gctgtaaata aaaatatggc taaaatggtg aataatatat
    22621 ctgacttcat attgaaatgt gagattgatg ttggcattaa aatagtttat gaagataaaa
    22681 ttaaaatcta tcatttaaag aaactaaaaa aaatatcaaa agaagcaaat gtagcattta
    22741 ttcatggaaa aggaaataga aaatctacta aaaaccttca tataatgggt gaacgaaata
    22801 gttgttctaa aacagataac gatgaaactt ttatgagaat gaaagaagat catatgaaaa
    22861 atgggcaact gaaaccagca tataatatac aatttggtgt agattaagaa tatatagttt
    22921 ggctaaattc aggaaatcaa actgctgata ctacaacatt aataccattt ttaaaatcta
    22981 tagaggaata tactaagttt aaatatccta aagtaatagc tgatggtgga tatgaaagtg
    23041 aataaaacta tgcattttta gagcataata atcagttatc tttcataaaa cctgcaaatt
    23101 atgaaattat taaaactaga aaatataaaa cttatattgg atgtattgac aatatggatt
    23161 atgaagaaga aaataattat tatatttgta caaatgagaa aaaactattt gctaataaaa
    23221 taataaatag gaaatcaaaa acaggatata aaagtgaaat aacatgttac atttgtgaag
    23281 agtgtagtaa ttgtcaatat aaaagcaatt gtattaagag aagtaatagt aaggttcctc
    23341 ttaaaaatag gactaaaaac ttacaagtat ctaagtcgtt tcatgaaaaa agaaaagaaa
    23401 acttaaagag aattatgagc ctcgaggggg atgaacttag agtgaataga agtatacaag
    23461 cagaaggagc tttcgctcaa gtaaaataaa atatgaaact tacaagattt ttaagccaag
    23521 gtaaagaaaa tgttttataa ggatatatta ttgtggcgat agcttataac gtttctagac
    23581 tacacaataa aatagtaaaa aataaacgtg gaatatatct ttatcattta aaaaaata
//
LOCUS       DAMKPG010000059        23369 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMKPG010000059 DAMKPG010000000
VERSION     DAMKPG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.062"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..763)
                     /locus_tag="PC621_002379"
     CDS             complement(<1..763)
                     /locus_tag="PC621_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF4109526.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            1037..1711
                     /gene="fsa"
                     /locus_tag="PC621_002380"
     CDS             1037..1711
                     /gene="fsa"
                     /locus_tag="PC621_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF4109527.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1833..3227)
                     /locus_tag="PC621_002381"
     CDS             complement(1833..3227)
                     /locus_tag="PC621_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF4109528.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3305..4651)
                     /locus_tag="PC621_002382"
     CDS             complement(3305..4651)
                     /locus_tag="PC621_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4109529.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
     regulatory      4901..5003
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5097..6473
                     /locus_tag="PC621_002383"
     CDS             5097..6473
                     /locus_tag="PC621_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF4109530.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6587..7732)
                     /locus_tag="PC621_002384"
     CDS             complement(6587..7732)
                     /locus_tag="PC621_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4109531.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            7984..8691
                     /locus_tag="PC621_002385"
     CDS             7984..8691
                     /locus_tag="PC621_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109532.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8738..10333
                     /locus_tag="PC621_002386"
     CDS             8738..10333
                     /locus_tag="PC621_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109533.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            10436..11518
                     /locus_tag="PC621_002387"
     CDS             10436..11518
                     /locus_tag="PC621_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF4109534.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            11667..11828
                     /locus_tag="PC621_002388"
     CDS             11667..11828
                     /locus_tag="PC621_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF4109535.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12169..13437
                     /locus_tag="PC621_002389"
     CDS             12169..13437
                     /locus_tag="PC621_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF4109536.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13632..14420
                     /locus_tag="PC621_002390"
     CDS             13632..14420
                     /locus_tag="PC621_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF4109537.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14420..14869
                     /locus_tag="PC621_002391"
     CDS             14420..14869
                     /locus_tag="PC621_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF4109538.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14873..17137
                     /locus_tag="PC621_002392"
     CDS             14873..17137
                     /locus_tag="PC621_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF4109539.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17277..17588
                     /locus_tag="PC621_002393"
     CDS             17277..17588
                     /locus_tag="PC621_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109540.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            17874..18374
                     /locus_tag="PC621_002394"
     CDS             17874..18374
                     /locus_tag="PC621_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF4109541.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            18431..19417
                     /locus_tag="PC621_002395"
     CDS             18431..19417
                     /locus_tag="PC621_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF4109542.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            19467..20189
                     /locus_tag="PC621_002396"
     CDS             19467..20189
                     /locus_tag="PC621_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF4109543.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            20218..22152
                     /locus_tag="PC621_002397"
     CDS             20218..22152
                     /locus_tag="PC621_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4109544.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            22145..>23369
                     /locus_tag="PC621_002398"
     CDS             22145..>23369
                     /locus_tag="PC621_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF4109545.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKIRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDECSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLGYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKN"
BASE COUNT         8974 a         2502 c         3781 g         8112 t
ORIGIN      
        1 ataaaacatt tacattaaat tctgataaaa tatctactat atcaggagta ggcgcttttt
       61 ctgtaattat agaactaata tcttctaaat gagcaaactt gaaatttcca tcaaatttaa
      121 actttttatc ttccataact ataaatactt ttttacttga atttattata gccttcttcg
      181 tgagaccatc ttcaatttca aaagttgtaa gtgaagagtt gaatacatct actccacaac
      241 ttcccataaa agtcttatca aacttatatt tcgatataaa ctctatagtt ggagtaccga
      301 caaaaccgtc taaactgaga tttaataaac ctccagtgga aattatattt aaattatttg
      361 gttttgaagt aactacattt agtatatcca acatatttgt aacaagtgtt acttttttat
      421 tactttcagc taacaacttt gcaagcaaga tgttaattgt agagatatct aaaaatatag
      481 tttctctatc ttcaattaaa ttaaatgctt tttctgctat tattttcttt gttggaatat
      541 taatttcttt tctgtctttt gtatcctgat tctctagtgt ttctctagct agtacagctc
      601 ccccataaat tcttcttatt gaagaaatct tttctatatt ttttaaatct tttcttatac
      661 aatcttctgt aacattaaat ttagaactga gctctttgac agtaactcta ccttcttttt
      721 ttaggatatt taatatagct tggattcttt cttctgcaaa cattttctac ctcttttcgc
      781 aatatagtat tatagtaaat attataagat aaaatacaat atatcaacta ttttaacatg
      841 aaatacaaaa ttaatttaat ataaaaagta tgttttttct aactatatat ttagaattta
      901 gtaaataaaa aaattataaa actaagaagg aaaagcaaaa caataataaa aaataaaaaa
      961 caataagcaa aatgttgaaa ataataccaa atcaatatat aataagaata aagaaagatt
     1021 aaagggggat attttaatgc aactaatctt agatacaggt aatgtggagg agattaagga
     1081 gttatgtact tgtttaccaa tagatggtgt aactacaaat ccaagtattg tttcaaaaga
     1141 aaaaaagaat tttaagcaac ttataaatga aattggtgag attataggtg aggatatgcc
     1201 tatacatgct caagtgttaa gtacaaagta tgaagaaata ttagacgaag cattatatat
     1261 aagtagtttg agaaaaaata tatatgtaaa aatacctgta acacaagatg gtttaagagc
     1321 tataaaagac cttcacagaa aaggtgtaaa aataactgct actgcaatat tcactgctca
     1381 tcaaggtttt ttagctgcaa aggctggagc aaattatatt gcaccttatg taaataggct
     1441 tgataatata tctggtgatg gagtagctat ggtttcagaa cttataaaaa taatagatac
     1501 ttataaaatg gatacaaaag tacttgcagc cagttttaaa aatgcacaac aagttataga
     1561 attaatgcaa catggggtgc atagtgttac agtaccatat gatatttgta aatctatgat
     1621 gaatcatcca ttaactgatt ggagtgtaga taagtttata gaggattggg agaatacttt
     1681 tggaaaagga agtaaaacaa ataatattta gtattatggt aaaagagtag ttttgtccaa
     1741 tttcacaaga acttatataa aaagatgaaa atatgaattt tctttttaat aatttcgtta
     1801 ttatcttaag ttaattcata ttttcatctt ttttagattt ctagtcctat aaatttccac
     1861 caagggaatg ttatacatat tattataaag taggttaaag gaatcaaagt taaaccgtat
     1921 ttgaaaataa ccttgctatt atacagattt tcaccatgac caaccattat tgtagcatga
     1981 tgaaatggta atatgtactg tatgcttact ataatgtaga caaatagaca taaagtaaca
     2041 ctattttcct ttagtatatg taaacttcca agtaatggta ttactacaga tgatgtagtg
     2101 acagcacttc caagacatat attcaagatt attgtgagag ttatcaaaaa caatattgaa
     2161 ataatttctg aagagtttgg tataaacttt atcaaataat ttcccattat atctacaact
     2221 ccagagtact ttaacactcc tccaattgaa aatgctgcta taaaaaaaat aaggaccttc
     2281 caatttattt cttttaaagt actaaatcca actatttttc ttagtaataa aagaagtaca
     2341 caaattgaca ttataattaa tgtgtttatg ttatgtatga actgagttaa aaacataaaa
     2401 aacattattc ctaggcataa aaatacgtgt ttttgctctt tgtttagcaa attaaattct
     2461 ttttttaaat tagaatatga aaaatgaagt ttattaccct caagattttc attagaaata
     2521 aattttattg aatgcaattc ttttttaaag acaaagataa ataaaaaata agttataaaa
     2581 caagtaatta tagttggaac agacatataa aaagcccatt gaccccaatt tatattacaa
     2641 tttccaagtt ctagtacaac ataatttaga agggtgtctc catttacaaa aaacattgat
     2701 gtaaaggtag atgctgtaaa tatactaaat aaaagtatag attttgaatc ttcagttatc
     2761 tgttgttctt ttaaaaactc cttgtaaaaa gcagatacaa gaataaccct tggaaatggt
     2821 tgtgggataa aaaatattaa aagaatacct aaaatatacg ataaaagaat catcttttta
     2881 ggggtattta cagtatttaa aattaacttc tcagaaataa tttttgcgac acctgttttt
     2941 gtaactgcat tagtaattat gtatgcaaga attattatgt aaaaattttc tgtaaataaa
     3001 aactgtaaaa taacctttat tggtgcaatg ttaaatataa acgctagaat tacaaaaaac
     3061 aatgcaacaa aagttttttc aactgagtct acagcccata tcaagatagt taaaaaagat
     3121 aagcatatag aaaattttat gttgaaactg taattaagag agaagtagag aattatggta
     3181 actataacag tcatataaaa ttgttttggc ttaagaataa tacccatttt tatattcacc
     3241 tccataattt attatatcat aatggaggtg aatacaatga atgtgaatac attttataaa
     3301 aagattattc attgattcta tgatttacaa tagctttagt tttccaatac cctttcttat
     3361 agtagataac tgtaacaata agacctaaag tccaaccaaa aggaataccc atccaaattc
     3421 cgtctgttcc aaaaaattta gaaagcatat aagaagctgg aagtcttcca agccataaag
     3481 atactattga gcttataagt gcaaacatgg aatcaccagc acctttcatt gcacttgaaa
     3541 gtacaaaaga agttccaata aataaaaaga atggtcctat tataaataag taagaagagc
     3601 caattttaat gacatcttta tcagtattga atagagcaat tagattttcc ctaaatagca
     3661 aaattaaagc aataactgta actgctaaaa ctaaggtcat ttttatagat tctcgaatgc
     3721 cttttttaac cctttcattt tctccagcac ctatgttttg agcaacaaaa gtagaaacag
     3781 cagaaccaag attcataata ggtaagctta taaaattttc tatcctaaga ccagcaccaa
     3841 aagctgccat agcagatgta ccataactat taactaaaaa ttgaagagtc atatttccaa
     3901 ttgaaaacaa catttgttga attccacttg gaagtcctaa tctaagtcca tctttaaata
     3961 ttttgttatc gtatacaatt cctttgattt taaatttaag cacttcatgt tttttattta
     4021 aataaattat acttattata aatgatactg cctgagccat tatagtggca agtgcaacgc
     4081 cctcaacacc cattcttagc actacaataa aagttaagtc taaaactata tttagaattg
     4141 tggctattat cagaaaataa agtggagtta ctgaatctcc aagtcctcta agtattgcac
     4201 ttactgaatt atatccagca gaaaataata ttccactaaa tattatagta agatacaatt
     4261 ttgattgagc aaaaacggat tctggtgtat tcataagctt aagcattggg ccaccaaata
     4321 ctattccaag aatacttatt aatatagaac caataaataa aagtatatat gtagtatcaa
     4381 tagtagcttt tacctttttt aaatctcctg agccataaaa ttgtgacact aaaatagaaa
     4441 atccaaggct aacacccata agcgctgcca ccaataaaaa cattataggg ttagctccag
     4501 agactgccgc cattgcttct tttccgataa atctaccaac tattatactg tcagctgtat
     4561 tgtatagttg ttgaaataaa ctaccaataa gcataggcat ggcaaaaaag aatatggatt
     4621 ttccctcatg acctgtagtt aaatctttca ccttattcct ccaaataaaa aaattattaa
     4681 tttattatag tataatctta acataaactt tatatccaaa ctataaataa attgtatcaa
     4741 aatatcatat atttttttaa aatgtaaaga atataattat aatttgaatt taataaatca
     4801 atatttaata taatcgaata ttaatacaaa atcaatacaa tatatacgta tatattgaca
     4861 aaattaatta tataaattaa aataatattc gtaaagaaaa attcatgttt tgtactgtat
     4921 aacatataat tttgacaata tgggtcataa gtttctaccg gaataccgta aatattctga
     4981 ctatgtatac tattatttag ttaattaaat atttaattaa ctgaattatg aaggatacgg
     5041 ctcagcttat caactactgt gtcgtttttt tgtaaaattt aataggaggt gttgttatgc
     5101 aaacaacaaa cagtagtact atgcagaaac tttttccaat tttgactaat aaaaatgtcg
     5161 acatgaagaa ggaaataatt gcaggagtaa ctactttttt aactatggct tatataatag
     5221 ctgtaaatcc aaatatttta tctgagacag gcatgcctgc aggtgcactt gttacaggta
     5281 cttgtttagc agcagcattt ggatgtattt taatgggtgt agttgctaac cttccttttg
     5341 ctttagcatc aggtatgggt cttaatgcat tttttgcata tacagtagtt ttacaaatgg
     5401 gtgttccttg ggaagtagct cttacagcag tatttgtaga aggtattatt tttatagtat
     5461 tatcagtaag tggagttcgt gaggctgttg taaatgcgat accaaaaaat atgaaattag
     5521 ctgttacagg tggtatagga atatttattg cacttatagg tctagttaac agtggaattg
     5581 ttattggaga ccaagccact cttatcaaaa tgggtagatt tacaccagca gtaataataa
     5641 cttgtttagg attaataatt attgctgtat tagataaaaa aagagttaaa ggttctatac
     5701 tttttggaat agtagtgagt tcattacttg cttgggcatt tgcttttatg aatccagagt
     5761 atgcacaaaa attaggtata tatttacctg gtggattatt taaattcgaa agtttagcac
     5821 caatagcagg taaaatagat ttaggatatg tactacatcc aacaaatata ggtggttttt
     5881 tagtagtagt ttgtacattt ttatttgttg atttttttga tacagtaggt acattagttg
     5941 gtgtttgttc taaagcaaac atgttagatg aaaaagggaa tgttcctaat gtaggtagag
     6001 ccttacttac agatgcggtt gcaactacag ttggtgcagg acttggagta tctacagtta
     6061 caacttatgt tgagagttca acaggagtta tcgcaggtgg aagaactgga tggacagcaa
     6121 taacagttgg aattttattc ttagctgcaa tgtttttctc tcctgtattt atagcaattc
     6181 catcatgtgc tacagcacca gctttaatat atgttggata tttaatgctt ggtacagtta
     6241 aggatataga gtttgataat ataactgaag gtgttccagc ttttgttact attgcttgta
     6301 tggcattaac atatagtata ggtgatggtt taactttagg tatattaact tatgttttcg
     6361 taaatatatt ttataatata tttggagcta aaaaggcaga agacaaaaaa catgtttctg
     6421 tagtaatgat tgttctggca atattattta taataaaact ttggctatta taataggtat
     6481 tatttaaaat tttaatttaa tataaaataa agggctataa tttattatca attgtgatga
     6541 taaactacag ctcttttgta tttgttgtat tttcgtattt attgtattat ttaataatgt
     6601 ctattagttc tccattttgt aaagttttag gtgtaattac tatactgaac tctttttgca
     6661 aatcttttat aatatctaat tctttttttg ttattattga gattgtgtta ccacttcgat
     6721 ttcctctaga agttcgacca catctatgca ggtattcatt tttacttttt ggaaaatcca
     6781 agttaaaaac atgacttaca tccacaatat ctagtccacg agcagataaa tcagttgtaa
     6841 ttaaaatctt tgctttacca agtttaaatt tatttattgc attctttcta tcttcttttt
     6901 ccatatttcc aaaaattcct attgctttgt aattatgata atttagtttt gatactagga
     6961 cttctatatt tttttcatta tttacaaata caatagcttt ttttggattt gtagctgcta
     7021 atgcctttct aagaaaagta aatttatccc ttatctcacc taataaatat gagtgattga
     7081 tgttaggatt tatttgtgat ttttcctttg tttttattat ttcaaactcc ttcataagtt
     7141 tatcacaaat ttttatagtg ctatcagtaa gacttgcaga gcatcctata atttgtctat
     7201 ctcttaacgt tgttcttatt atgtcttcta tacaagttat attttttcca ttcaatagat
     7261 tgtccacttc atctaagact atagttttta tattatgtga ttttagcttc ttttgtttta
     7321 tcaagtctaa aactcttcca caagagccaa ttactatgtg tggttttact gcttttatat
     7381 tttttatttg tttttgtata ttgacctccc caattaaagc taaagaagta acggagagtt
     7441 ctgcattttt agccaataat tcaacctggt ttgtaatctg cattactaat tcatgcgtag
     7501 gtgcaagtat aagagcttgt gtttctcttt ttgatgtatc aattttttca aatataggta
     7561 aaaggtacgc tagtgttttt cctgttccag tctgtgaatt aatcaataaa tccttatttt
     7621 gaattatatt ccctataaca agtgactgaa cttctgttgg agaagttatg tcttgttttt
     7681 taagtccatc tattagagtg gaacttattt ttaattgttc aaatgtattc atattttgct
     7741 ccttatattt aataattata gtcaactatt ttttattata cattgtttat tcaaatcgaa
     7801 ctatatattc ttttataatt atgtgtaatt tgaaagtaaa tatcaattat atttatacct
     7861 ttatatttct gatacctttg cattttatag atttctaaaa taaacaaata aaagttgtaa
     7921 ttgtttgata tttgttatat attatatata acaaatatca aaactataat atggagggaa
     7981 attatgttaa aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt agtaagtagt
     8041 gtctcatttg atgtaaatga tggagagctt tttgggttta taggacataa tggagcaggt
     8101 aaaactacta ctattaaagc aattgttgga atacacgatt ttgaagaggg agaagtacta
     8161 ataaattcta aatctataaa aaaacagcct gttgaatgta aaaaggaaat ggcatatata
     8221 cctgataatc cagatttata cgagtcatta acgggtatac aataccttaa ttttatagct
     8281 gacatatttg aagttgccaa agacaaaaga gaagagttaa taaaatatta ttcaaataag
     8341 tttgaactca ataaagcatt aggagattta atatcttcat attcgcatgg tatgaaacaa
     8401 aaattagcta taatttcggc tttgatacac agcccaaaaa tcttaattct tgatgaacct
     8461 tttgtaggtc ttgacccaaa agcttccttt atattgaagg aaattatgag ggagttttgt
     8521 gacaaaggtg gttgtatatt tttctcaaca cacgttttag aagttgctga aaaaatttgt
     8581 gataagattg ccataataaa agatggaaat ataattgctt atggaactac agaagaagtt
     8641 aagggaagta attcacttga aagtatcttt atggaattaa tagagaaata aaatgttttt
     8701 tatacaatat agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa gtatattaat
     8761 aaaaactaat ataataaatg aatttaaatt aaatgcgtta aaaaaagctg atagtagaga
     8821 aaaacaaaaa ataatgggga tgatactttc agttgttatt ataacagggt tattgatttt
     8881 ttatgtaact agtataagtc tttctttagt tgatatttta aagcaaataa atcagatgga
     8941 aatgctactt atatttggat ttggattatc aacattaaca actattgtta catcattata
     9001 taaaacgtca tcatatctgt ttcaagcaaa agatttagac cttttgacta gtttacctat
     9061 aaaagaatct actgtactag caagtaagat attaatgttg gttggagcaa actatttatt
     9121 ttcttcaata tgtatgatta taccagcagt ggtttatttt tttaattctg atgtaagtat
     9181 aacgtatttc ccttatttga tactgctatt tttagcttta ccattattac caatagttgc
     9241 ttcatcaata atttttatgt ttataggaca gatatcatca agaataaaat acaaaaattt
     9301 aatattaatt ataggtagta ttatattgat gttgctttat actctactta tgacaagact
     9361 taactctatg tcggttgaga tgttaaagaa tagtgtatct atttcagaga cgattagaaa
     9421 aatatatcca cctatatatt attttgttga cgctcttaaa acaggaagtg tagtttcctt
     9481 tttagcattt atagcaattt caataatacc attttttatg tttgtaacat tatttgcaaa
     9541 aaaatttaaa gatataaatg ctaagatgag tgaaggatat aagtcaaaaa cttataaatt
     9601 taagagccaa aaaacttcaa gaccttcaaa agctctatta aaaaaagaat ttaagagata
     9661 tacttcttca tttatatact ttttaaattc atctgcaggt ttgttattcc taataatagg
     9721 tacaatttta atattattat ttggagcaga taaagtgggt gagttactga aattgaatat
     9781 agattttaat tttataaaaa tacaattatt gggagtattc ttctttataa tagtaatgaa
     9841 ttgtacaaca tactgttcaa tttctcttga aggaaaaaat ttatggatac tgaaatcttc
     9901 tccaatagat gaaagagaaa tattctggtc aaaaatattg atgaattttt tattgaatgc
     9961 accattatct attttatgtt taatattagt atctataaga ttaggttttg aaattcagtt
    10021 tattattgta tcaatctgtt tgattgcaag tttttcaatg tttgtggcat tgatgggatt
    10081 attatcaaat ttattatatc caaaccttga ttggaaaaat gagatggctg tagtaaaaag
    10141 aagtgcaagt atgataatta caatgttagt atcattagct tacgtaatta tacttggagg
    10201 tatatatgta ttacttggaa tttcgttttt aaatatatat attattttgg caagtatact
    10261 tacttttata ttagatttta tgatatggag aattatttct actaaaggag taagaatatt
    10321 taaaaattta taggtgtatt tatgtcaaat aaagacttat cttttttagt cttgcaaata
    10381 ataggttaag tagtatacta tgtatagtga tatgaaaaaa tttaggagaa aataaatgag
    10441 aaaattatcc aaaagagaaa aagattttat tcaatatgga tttttattat ttttgatatg
    10501 tattactact tatttagtat taactacttt agatataaaa ctaataccta aaattataag
    10561 gctagtaaat aagacttata tatttcttgg catattgaca attatagtat atatgataat
    10621 tgaatctatt gtaactcatt tgataataaa tgcagttcaa aaaacaaaag taaaatttat
    10681 tggtattaaa atggctacaa tgggatttta ctataactta gttactccat ttgcatctgg
    10741 gagtcaacca atgcaaatat atgcacttac aaaatatgat gtaagcctta gtaaatcagt
    10801 ggctatagtt actaataaaa ctgtagtatt tcaaagtaca gttactgtat tttgtggaat
    10861 tttaattttg ctaaataagt cattactgat ggagcaagta cattctgtga aagtcttgat
    10921 aacttttgga atgattatga atgtaagtat gttgttaggt ggactattaa tagtatttag
    10981 tccaaaacaa gtaaaaatga tagcagaagt tttgattaat tttttatcta aatttaaaac
    11041 atttaaattt ttagattcaa aacgtgatag aataaatcat tatattgatg attataatta
    11101 ttctataaaa atatttatta aaaatgtaag aatcttagtt ttaagtatat tacttactgt
    11161 gttacaatta atcgcatatt tcagtatagt ttattgtgtg tataaagcct caaatttaag
    11221 aggagtttca tatgtacata taatgacatt acaggtcttt ttatatatgg ctatatcacc
    11281 aattccaaca ccaggaaatg ttggagctaa tgaggttaca ttttttacta tgttctccaa
    11341 tataattcca aaagaattat tggggtactc tgtactctta tatagtgggt ttgtatacta
    11401 ttttatatta atagtttcag gtttatttac atttggaacg cattatacat taaattcttg
    11461 gaaaaaggaa gataaagtaa attctaattt agataaggtt attgaaaaat taaattaata
    11521 aaataattag ggaaattcta tatataaata tggtgttgag taagctatat tatattgaga
    11581 tatttccctt ttttgattaa aattatagaa atttgaaata ataagtaata aacagaaaaa
    11641 gtgatattgt tttgaaagga gaaactatga aacaagataa acaaaatgaa aaacaaagtg
    11701 agatttctaa gataaaacaa agcaatgata ataaacttga aatagctcaa gaattacatg
    11761 taagtaaaaa tatagggaat atgcttggat atagaataga taagaaaaaa atgaatagac
    11821 caagttaatg aaaatgttga aaatggtaca ttttaaaaaa tatatataat gaaatgtttt
    11881 attgttaaat ttaaacttat tttaatggag gaaaaagtcg aaaaatatct aaaaagatat
    11941 ggatataaaa aatattaata tatatatact ataaacttga caaaatcaac cttttaataa
    12001 ctagcaaaaa atagcaaaat tatataagcg ttatttaatt atcaaaaatg aaagctaaat
    12061 tgcatgtcct aaaaaaaatg attgataaca tttatttgtg tgctataatt atatagtaaa
    12121 acaataagta tattgcataa tgtatgcaaa tcgggaggag agacggacat ggaagcatct
    12181 atacacacaa ttgccagcaa taatttattg ataatttttg ctattgtaag tataactgga
    12241 ataatttgta gtaagcttag tgaaataata aaggttccag atgtagtgtt atatttactt
    12301 gtaggtattt taataggacc atcattttta aaatttatag atattcgtgg atttcaaata
    12361 gaaaatcaac taatacttac atttggttct gcatttattt tatatttagg tggaaaagaa
    12421 ataagtttaa aagtattgag aaatgtgaag atatctgtat tcttattagc tactttagga
    12481 gttgtgatat ctgcttttat aatgcaacaa gtaataggat ttacttttgg gataagtgca
    12541 atgactgctt tactagcagg aactataata gcatcaacag acccagccac tcttgtacct
    12601 atatttaatc aggtaaagat aaaggatagg gtaaaacaaa ctgtaataag tgaatcagct
    12661 tttaatgatg caacaggagc tatactaaca tcagcagtgt tggctgtaat tttgtcaaat
    12721 aaattttcat taggtgaaaa tatttatgaa ctggggacaa tggttattgt cggagtttta
    12781 gttggtttaa ttacaggaat actgcttttg aaattagtta atgataaacc ttatggaata
    12841 tttaaagatt ttgcaccaat aatatctgtt atttcagttg tagttgcata tgaattatct
    12901 acaaaattag gtggaagtgg atatatgtct tgctttattg taggtataat aactggtaac
    12961 aagaaaaact ttaaaatttg gttaagtcaa aaatcttatg atgcagattt ttatgttgct
    13021 gaaactttag gtactatttg tcgtatggca atatttataa tattgggtag ccaagttaat
    13081 ttggctcagt taagtaaata ttttatgcca tctgtaatta cagtattagt attaatgttt
    13141 atagcaagac cactttgcgt tttagtttgt acacttgtag acagggaagc aaaatggagt
    13201 aaaagtgaaa tattatttat gatgtgggta agggaaacag gcgtaatacc agcagcactt
    13261 tgtggtatta tttcagcaat gaaagttcct ggatacgaag tgatttcatc tgtagtattt
    13321 atgactatat taattacatt agtaatacaa ggaagtacta caaaattagt agctaaaaaa
    13381 cttggattat tagaagaaga agtgataaat ataagtgaaa aagtatcaac tttttaaagt
    13441 tttgctttaa aatagtacct gttaatggct attttaatat tgtctaatga tgattattga
    13501 aatagtcatt tttttatgta taagtaattt acagaattta catattagac atatttatta
    13561 tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat agcaaataag
    13621 ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta gaagaagcgt
    13681 actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct tttttaagaa
    13741 tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta gattatataa
    13801 aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca ttagaaacaa
    13861 gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa aatataatag
    13921 gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa tatggttttt
    13981 ctgacttgat agtagcatta ctttcattag atacggaagt agaattatat aatattggga
    14041 gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt attaagatat
    14101 acataaagaa gacaaataag aatgcttctt ataaatcact tagaaatgca aagagtgatt
    14161 atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta tgtgtaggag
    14221 caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct aacaatgaaa
    14281 tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta acttttggtt
    14341 ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt ttaaaaagag
    14401 ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga aaaaaagtgt
    14461 tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta atttaagtgt
    14521 gaaaagaggt tgtgacacag gctgttgtgg tttatgtaca gttttaattg acaagaaacc
    14581 tacattatcc tgtgcaacac tgacattgag agctttagat aaagaaataa ctacaattga
    14641 aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag gagctgagca
    14701 atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg atgagcttga
    14761 aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta gatgtacagg
    14821 gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga acatgaatat
    14881 agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa agcctgtata
    14941 tacagatgat ttatcagcaa aaaatgcttt ggtggtaaag atacttagaa gtcctcacgc
    15001 ttatgcaaaa ataaaagata tagatacatc aaaggcagaa atggttgatg gtgttgaatg
    15061 tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc aaacatatcc
    15121 agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg taggtgacga
    15181 agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga atcttatcaa
    15241 agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag gaaataaaac
    15301 tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa gagaaaaaaa
    15361 tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa gtaattgtga
    15421 tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga tggagacgta
    15481 tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt caactcaaat
    15541 accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta gcaaaattag
    15601 ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcgt gtgttgagat
    15661 tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt atgatagaaa
    15721 agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta gactaggtgc
    15781 tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata caggagcata
    15841 tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac caatgtataa
    15901 taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga tgccagcagg
    15961 agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa ctataaatat
    16021 gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg tggtaacaga
    16081 aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa taaaatgtat
    16141 aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac atcttgataa
    16201 tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta tacaaggtct
    16261 tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc ttatgatagg
    16321 ttctactgat atgggtacag gaagtgatac gattttagct caaatggctg ctgaggttct
    16381 tgatacaagc attgaaaata taacagttat atcagctgat actgacttag ttccatatga
    16441 cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag ttaaggcagc
    16501 tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg ttgagaagga
    16561 tgaagtagaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt tattattcga
    16621 tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc ttgcttctca
    16681 tggtagccca gtatctcctc caccttttat agcaggtttt gcagaaactg aaattgacac
    16741 agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg gaacggttat
    16801 aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga ttggaatggc
    16861 attatttgaa gaagttagat acacagaaaa aggaagcatg gacacaaata catttatgca
    16921 atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg ttgaaactta
    16981 tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa atacaccttt
    17041 accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata cactccctat
    17101 tacacctgaa aaagttttta tggaaatgaa taaataatta tttatgaaaa tttagactag
    17161 ttttacatgt taaacaacca gttatgtcat acctgttgaa agatatttaa taagtagtta
    17221 ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat gatgttttgg
    17281 ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag aacattttag
    17341 aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg gtatttttta
    17401 aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct aaattggttg
    17461 aaaatgttgt tttaaataca tcatatcaag aaatgttata tgatgaaata gagaaacaac
    17521 tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat ggtagtagag
    17581 ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt tgctgttttt
    17641 aattaacaat agaaaattta tctttagttt gtatataatt ttaatatcat atattctata
    17701 tatataaaat tgaatttgta tgtattatat tgtataatat aaaatgaatt tggaaaatag
    17761 ttcgctaaaa taatttgttt ttataaaatt actgtttaag tttatagatt ttgtataaaa
    17821 taaagttatg ttattaattt ttttagaaat aaaactttta ctaggagaca gatatgagaa
    17881 aaaatagtat taatatagca ctaactgcag caattacagc aatctatgca gttttaacat
    17941 tatcattggg atttataagt tatggaccaa tacaatttag ggtagcagag attatgatgc
    18001 ttttggcatt tttagataaa ggatatatag taggtcttac attaggttgt tttttagcaa
    18061 atgtaatagg accatatggt gtacctgata taatttctgg aacattagcc acatttataa
    18121 gtgcaagtat gatttatatt actagaaagt taggtaaaca aaataaaagt actttagtta
    18181 tagcttcttt atggccaact gttataaatt cgattgtggt tggtctaatg ttaaatgtat
    18241 tctttgggct tccattaatt ctatctatga tacaggtagg ttttggagaa tttgtagtag
    18301 taacaattgt aggagtacca tttttctgct ttttaatgaa aaaatataaa aatgtcatta
    18361 atattaaatt ttaaatagtg tatagtaatt taattaattg agagtattat tagtgaaaag
    18421 aggtgtttat ttgtcgaagg taaaaagatt gactgatgtt tggatatata ttaatgataa
    18481 gcaaaagttt acaataaaag agttgtcaga ggaatttaat ctttcagcta aaacaataca
    18541 acgatattta aaagaattaa ataaaatggg acttccaatt caagcagaac aaggtagaaa
    18601 tggaggttat agggtgctaa ataatagcta tatacctcct gttatattta cagaaaaaga
    18661 agttatgatt atactatttg cacttaagtc acttcagatt tataattata agttaatgga
    18721 aatagaaatt aattctataa tgagaaaagt aagttatgaa cgtaaaagca gtataaaagt
    18781 aggtatagaa aatattaaaa gatacattgg ctttgtagct aatgatacta atgatggaga
    18841 ttttaagagt agtaatgtaa ctgagttttt tagagcttct tcagaatctc taattttaaa
    18901 tattaagtat aagtgtggta atcagatttt ggaaaaaaac gtatgcccta taggtatata
    18961 tttaaataag ggagcatggt tttcaccagc atatgattgt aaaagtgata aaataatatt
    19021 agttcatata gataatataa ttgatataaa aagaacagga gaaagtaaaa aaatacatat
    19081 aaacttggaa gaatggttac atgaagtata taacaattta tatggaaata gttcaactac
    19141 taaaaaattt ggtgaaatta tttatctaaa tgtattatta actaaagaag gtgtttctaa
    19201 aataaaggac agttcttgga atttagatat ggagctaaat gaagatggta gtggaataat
    19261 tagtacattc ataaaggttg aagatataga ttgttatgct tcgattttat tttttattgg
    19321 aaataatgct aaggttatag agccaaaatt tttgaataag ctactttatg ataaggccaa
    19381 tgagttaaga tatttttatg aagaaaatat ggtataatat ttagaaatgt tgaaattaaa
    19441 taattattaa ggagatatac gttataatgg aaaattttaa taagaatgct aaaataattg
    19501 gtattggagt tgaaggtatt aatataataa atgaagtaga agaaaaaata aaagcaaata
    19561 tggatataga aaaaataaac ataaatcaag agattgaaaa agaatatgtt agaagtcttt
    19621 tagatggtgt agatatatta tttttgatat attcatctga agataaacat attagagata
    19681 ttataaaagc tgtttcatat atgtcaaacg aaagaagagt tctatctata ggaatggatt
    19741 gttcagagaa agaaaataaa gaagatttag agcttgggag agagtttaaa atcaataatg
    19801 atagtatatt taaatttgtt gatttaatga atatcatggt agagtctatt tctgattctt
    19861 gcatgataaa tatagatatt actgatttaa aagaagctat tgtaggagat aaaggaatta
    19921 agtattcttt tgaagagttt gaagatacaa agtcatatag cgagatagct gacattttat
    19981 ttgacaggat ggaatatatt ggtgaggaat ttataagtaa gaaaggaatt gtatttgttg
    20041 aaggaagtcc agaattttct ataatggaat tgaatgattt aataagtaat attcagagca
    20101 aggtagaaga aagctatgaa gtaatatttt ctttatatat aaaggaaaat ttaaatggaa
    20161 atattagagt aggcttattg tataattagt atattttaga tattaaggag agtgtatatg
    20221 tctgaaaata taagaggtac agagtattgg ttaatggaag aattttttta tcaggaaaca
    20281 gacacaccac agatttttat agaagaaagc ataattgcac ttggatttga tataaaaata
    20341 aatttaaaaa aagtattcag tatggggaaa aaatcagaaa agtatattaa tgagaattgt
    20401 aatgataaag attttataaa aaagtttatg tcgattaaaa aaggagatta ttttttatat
    20461 aaagcatatc aaaatataga agaaaaagtt aaatgtaatg tgtctattgg catagttgaa
    20521 gaagattttg aaaatggata tgaattaagt catattttag ggcatacact accagtaaaa
    20581 tggatgcata attttgatac agtttcaaat tgtaaagtat atacaagtga attttataaa
    20641 ttaaataata atgatattag ttcatattta gaaattatta agtcacaaga taagaaatta
    20701 ttgagaaaaa gaatagaaga aagtgaaaat aataaatggt atttaaaaaa ctattattat
    20761 aaaaatgtag ataagtgtgt agatgatgaa gaattattta ttcagcccat taatctagaa
    20821 caattagaaa gcctaaaaaa tataaagaca gatgattata ttttattgta taatagcgag
    20881 aaagtaattt gtgaaaatga ccatacaatg aagagtctat ttggcgtatg tcaagttatg
    20941 gaagggttcg ataaagagaa agtaagtgct ttggatggtg gatatttttt tcctgttaga
    21001 tggatagagt atggaaagga gaatatagac ggaaatgcat gggttatatt tgaaaataat
    21061 agtatagttg aaaagtatgt aaaaagattt actaataaaa tgccattaaa tactatactt
    21121 tatggaccac caggaactgg taaaacatac aatacaaaac agatggttta caatctagta
    21181 agtgaaacca gtctaagaaa caaatactat acaggttact tagattataa aaacaatgta
    21241 gaattttgta catttcatca atcatatgga tatgaggaat ttatagaggg tcttaagtct
    21301 gatggtgaag gcaattttaa gccagaagat gggatattaa aggatatatc tatagaagca
    21361 tgttataatg cattgaaatt tgaaaaaaag atgagctttg agttggaaaa agatagcttg
    21421 actttagaaa ctataaaaga caggaaaaag aaattagtat tagaaaatat agaatatagt
    21481 gagagtttta attttgaaga atcagaaaac tatgttttaa taatagatga aattaataga
    21541 gggaatgtat ctaaaatatt tggtgagcta ataacattat tggaagatga taaaagactt
    21601 actaaatcaa atcaaactat tgtcaaacta ccatattcaa aggaaaaatt ttctctacct
    21661 ccaaatttgt atatagttgg aactatgaac acatcagata aatcaatagc acttttagat
    21721 atagctttaa gaagaaggtt taattttgaa gaaattatgc cagattatga tttattgtct
    21781 attgtagatg gtatagatat tagaaaaatg ctatatacaa tcaataaaag gatagagttt
    21841 ttatatgata gagaccatgt tattggacaa gcttatctac ttgctgtaga aaatattaat
    21901 gatatagtag tagtatttag aagtaaaata ataccacttc ttcaagagta tttttattat
    21961 gattcagaaa aagtaggttt gattttagga ggaattggaa gaaatgaaaa agataaatac
    22021 attgtttata aagaaaaact tatagcttct gagttattta aaaataataa tgcctcaata
    22081 atggagagta aagttaacta ttgtataaaa caaaacatat ctagtgagga gctaaaaaat
    22141 atatatgagt aaaaatatat atgttaagga aacctatgaa tggataaggg taggtaatgg
    22201 tgaaaatgag cttacagaaa ttgaatatga aaaattgctt aaatatctag agaacaataa
    22261 tgatgtatta aaaagtaata taatagatat taaatacaaa aaaattagat ttataaacta
    22321 tgtaggcatc atatgttttg aaaatgtaat acttgaaata cttcctaaat tgtcattaag
    22381 tgataatctt gtaaaagata gagagatact attacagatg ctttctatat gtaataaaat
    22441 tccaattaca atgaatgaaa aaataagatt gagtttgaaa aattataatc tcttaaattt
    22501 ttttgttatg tatttcattg agagtatgca aacacagatg aaaaaaggta tttattttga
    22561 atatataaat aaaatagaga acttaaatgt tatgaggggt aaaatattat taagtacata
    22621 tgcaaaagaa aaaggcattt ctccaatgaa aataagatgt gaatatgatg agtgtagtga
    22681 aaataatttc ttgaatcaag ttcttaaaaa agcatgtata tctattttat gtagaataaa
    22741 tgacaattca attcaaggca agattaaaaa aattttaagc tattttcaaa atgtagattt
    22801 aatttatata gatagaaaaa aactgttggg ttataaattt tataaaaaca atgatagatt
    22861 taaagattgt tatttactag ctagacttct acttttaaat ctatctatgg ataatagtca
    22921 aaataatcaa gaggcatttt ctatattatt tgaaataaat actttgtatg aagaatatat
    22981 aggaatttta atcaaaagta tatgggacaa ttcatttaga gaaacatata tacaagataa
    23041 gagtaaattt cttttgaaaa atgaacaaac aggtaagaaa aactttaatt taagaccgga
    23101 tatagtttta tatgacttaa aaaatgaata tgaaattata attgacacta aatggaaagc
    23161 tattgaagta gattcaaatg ttttttatag gtcaagtgat atataccaga tgtatgcata
    23221 tataacagca tatgaaaatg caaaaagatg tatattatta tatccttgta ttcaaaaaga
    23281 taagaattat agttcctgga aattatcaga atcatttaag ggtaagttta tagaagcaaa
    23341 aactgtgagg ttagatgata taaaaaaca
//
LOCUS       DAMKPG010000060        23052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMKPG010000060 DAMKPG010000000
VERSION     DAMKPG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.063"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(79..645)
                     /locus_tag="PC621_002399"
     CDS             complement(79..645)
                     /locus_tag="PC621_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF4109546.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            811..1332
                     /locus_tag="PC621_002400"
     CDS             811..1332
                     /locus_tag="PC621_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF4109547.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2123..4201
                     /locus_tag="PC621_002401"
     CDS             2123..4201
                     /locus_tag="PC621_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF4109548.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4201..4980
                     /locus_tag="PC621_002402"
     CDS             4201..4980
                     /locus_tag="PC621_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4109549.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            4973..5782
                     /locus_tag="PC621_002403"
     CDS             4973..5782
                     /locus_tag="PC621_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF4109550.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5802..6284
                     /locus_tag="PC621_002404"
     CDS             5802..6284
                     /locus_tag="PC621_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4109551.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6305..6709
                     /locus_tag="PC621_002405"
     CDS             6305..6709
                     /locus_tag="PC621_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4109552.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(6865..8025)
                     /locus_tag="PC621_002406"
     CDS             complement(6865..8025)
                     /locus_tag="PC621_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF4109553.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8233..9783
                     /gene="xylB"
                     /locus_tag="PC621_002407"
     CDS             8233..9783
                     /gene="xylB"
                     /locus_tag="PC621_002407"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF4109554.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            9879..11216
                     /gene="xylA"
                     /locus_tag="PC621_002408"
     CDS             9879..11216
                     /gene="xylA"
                     /locus_tag="PC621_002408"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF4109555.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            11738..12937
                     /locus_tag="PC621_002409"
     CDS             11738..12937
                     /locus_tag="PC621_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF4109556.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            13104..13889
                     /locus_tag="PC621_002410"
     CDS             13104..13889
                     /locus_tag="PC621_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4109557.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            14058..15623
                     /locus_tag="PC621_002411"
     CDS             14058..15623
                     /locus_tag="PC621_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4109558.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            15637..16968
                     /locus_tag="PC621_002412"
     CDS             15637..16968
                     /locus_tag="PC621_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF4109559.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            16982..17470
                     /locus_tag="PC621_002413"
     CDS             16982..17470
                     /locus_tag="PC621_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109560.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            17484..17951
                     /locus_tag="PC621_002414"
     CDS             17484..17951
                     /locus_tag="PC621_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4109561.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            17954..19108
                     /locus_tag="PC621_002415"
     CDS             17954..19108
                     /locus_tag="PC621_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4109562.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGCLAYGLIVLLNYLQE"
     gene            19426..20178
                     /locus_tag="PC621_002416"
     CDS             19426..20178
                     /locus_tag="PC621_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109563.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            20179..22086
                     /locus_tag="PC621_002417"
     CDS             20179..22086
                     /locus_tag="PC621_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4109564.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKFKSVVYKN"
     gene            22238..22912
                     /locus_tag="PC621_002418"
     CDS             22238..22912
                     /locus_tag="PC621_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109565.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
BASE COUNT         8907 a         2457 c         4016 g         7672 t
ORIGIN      
        1 attattaata aaaatgttat ataaaaatga gtatatagca ttttaaagag taataaatct
       61 tacataataa atgatttatt aatatgaact agaagtattt ttactatatt gttttataaa
      121 acctttaggc attttactta taaaatatac caccataata caagacaaag gtttgtctac
      181 tatattacta agaatccttg gaaagaacgc agcagtaaat atcttctgac cagactgaac
      241 taaaaaacca accaataaat cagcaccact tccacttaaa ccaccaaaca ataaaactga
      301 aataggagta ccaattaaag gacaaacaat agataaaatt actcctgtaa taatagcttc
      361 tttataacca aaaccttttt tcttagaaat atatccaaca ataagaccaa gaacaatatt
      421 tacaagagca aaaggtatat ttgtaggacc tgatataaca cctaaaacta agtttgtaca
      481 cccaccaata agagcacccc aaaaaggacc taatacgaca gccccaagta cagtaccaac
      541 tgtatctaaa aataataatg gtatatttat catagatata accataccta agacaatatt
      601 aataactatg catattgaag aaaatgtgac ttttttagta tccaattata atacctccta
      661 aaaaaatata tacatttatc atagtataga ttataaaaga ttactaattg tatatttcta
      721 tatagaatat tggaaatata gaacaaataa ataatataat tatcaaaata tgtagtatca
      781 ttaaaattaa gaataactaa ggagattatt atgaagagaa atagagagat tgtagtagta
      841 tgtcattgta tattaaattg taattctaaa gtagagggat tgtcagaatt taagggagca
      901 caaaatatag cgataaattt aataaataaa gggtatggaa taattcaact accatgccca
      961 gaaatgatta tgtatggaat aaataggtgg ggacatgtaa aagaacagtt tgacaatttg
     1021 ttttttagag aacaatgtag gcaaatgtta atacctcatg taaggcagtt taatgaatat
     1081 ttaaaaaatg attatgatat aaaaagtatc attgcaatag atggtagtcc aagttgtggt
     1141 tataataaga catgtagttc taaacgttgg tttggtgagc taagtggttg caatgaattg
     1201 atggataaaa taaatgatat acaaatggtg gatgggaaag gtatttttat agaagagcta
     1261 gaaaagctac ttaaagaata taatttagag atacctatag ttggatttga cgagcttaga
     1321 gaagataagt aaagatataa atatttattg tctcattatg aaacaccatt atatttggca
     1381 gaaaaatatg actgatggac agtacctgaa tcatttttaa gtggagtaat tagtacttat
     1441 aaagatgtat ctactagctg aaagaaaatg tagagaggta ataaatgaat tgataatata
     1501 tataaaggag tgtctaaaag tgaacttttt agtttatgag acactctttt tttatattaa
     1561 atcaagtata ttaaatcaaa tatattttaa tcattttagc aatgaaaaat aaatttatct
     1621 atattttgag acaacatctt ttttagtttt atttatttgt tgtcgttttt gacttggtat
     1681 acaattatat attttcttaa ataatttcat aaattattta taattataaa atccattgtt
     1741 ttgaacaata tctaatatat tattatctgt atttataagg tcactataca tattttcaag
     1801 tccaatatta tctacataat aggcaaattt tattcctgta tgtgtttaaa aaaaactaga
     1861 tgtatatagg tatcatcaca gatattaaaa gtataaataa aagatattaa gaacgacttt
     1921 caagcaacat attagaaaat tttactaaaa tccggaaagc gattgacaaa caaagtcaaa
     1981 taaagtatat tattggtaag ttagtttaat gtacaaatga attatatata aaacctaatt
     2041 tataaataac gatactaaac gacattattt aacttaagct acacaaagat agaatattat
     2101 accattgatt taggaggtag ccgtgataaa aatatctgaa gactatattg aaaaaagatt
     2161 tgacaatgaa ctattgcgaa tagaagcatg gggaaaaaac tctttacgta ttaggtcgtt
     2221 tgtagaccaa aattttgttg atgaaaacta tgctcttaac gaaaaaccta agttaaataa
     2281 agatgatatt acaattaata aaaatgaaga tggaagtgca atcattaaaa atggaaagat
     2341 aaaagctgtc ttagaccata gagatagaat tactttttat aatgataaaa atgaaatatt
     2401 attgaaagaa tatattcgat taagagctgt aaaacatgat gatggtggag aagatgtagg
     2461 gacaattgag attaccaaag actttaactc tactctaaag ttaaaatcaa gagaatatcg
     2521 accaagctat agtggagaat ttgaagtaac tactagattt gaatctgagc cttctgaaaa
     2581 gatttttgga atgggacaat atcaacataa atttttagat ttaaagaata ctgttttgga
     2641 gttagcccaa agaaattctc aaatatcagt tcctttttat atttctagtc taggttatgg
     2701 acttttatgg aataatccgg gaattggaaa agtatctttt gctaaaaata tgacagaatg
     2761 gaagatgttt tctacaaatt ttatagatta ttggattact tgtggagaaa gcccaaaaga
     2821 gttaaataaa aattattctc aagttactgg aactgttcct atgatgcctg aaaacctttt
     2881 aggactatgg caaagtaaac taagatatag aacttcagaa gaagttttag atgttgtgaa
     2941 ggaatattct aaaagaggaa ttaagctttc atcaattgca attgactatt ttcattggcc
     3001 taagcaaggt gaatataaat ttgacttaga ttattggaaa aatcctaaag aattagttaa
     3061 aaaattgaaa gaagagtatt cagtagaacc aatagtatct gtatggccta cagttcaaag
     3121 tgatgcagaa aactacaatg attatttaga aaatggctat ttagtcaatg ttaatcgtgg
     3181 tgttagaatg acaatgcaaa ttcaaggaaa tacagtcttt gtagatatga caaatgaaaa
     3241 tgcaagagaa tatgtttggg atagaataga taaaaattat aaacaacttg ggattgacta
     3301 ttattggtta gatgtagctg aacctggcta ttctgtgtat gattttgata actatagata
     3361 taaaaaagga aatgttttaa gttgtggtaa tatttatcct attgattatt taaaaatgat
     3421 ttatgatggt ttacataatg acatagagtc tgttgtaact cttgtaagag gagcttgggc
     3481 aggagctcaa aagtatggag ctttagtatg gtctggtgac attgattcta gttttgaggc
     3541 ttttaataat caagtaaata cagggttaaa tatgggactg gcaggtatac catggtggac
     3601 tactgatatt ggaggtttcc atggaggaaa tcctaaagac ccagaattta gagaacttat
     3661 ggtaagatgg ttccaatatg ctactttttc accaattttg agaatgcatg gagatagatt
     3721 gcctcatagt aaaccattat caaataaagg tggaggctca atggtaactg gagctcctaa
     3781 tgaaatatgg tcttatggtg aagaagttga agtaattctt acaaaattca taaaaattag
     3841 agagagctta aaaacttatt taaaaaaact gatgaaagaa gcgcatgaag atggaacacc
     3901 agttatgaga actttatttt atgaatttcc tgaagatgat aaaacttggg aggtggataa
     3961 tacatatatg cttggagatg aaattttggt agctccaata atgaattata aagatagaag
     4021 tagaaaagta tatttaccaa aaggacatac atgggaaaat atatttagtg gagttagtta
     4081 tgaaggtggg aaaacttatg aagttgaatg cccgctggaa gaaattccta ttttcctaaa
     4141 gcaagattcc agttataatt ttaaagaact aaaaaatatt ttgggagagg tgaaatcata
     4201 atggagccac aattatggca attattatta atcgtactat atggattttt tataaactat
     4261 gaaaaaaatt caacaatgtt tggaacatat caaccagtaa cagcaggttt tattactggt
     4321 cttatcttag gagatataaa tacagggtta tatattggag gtacattaca gctattatca
     4381 ttaggtatta gtaactttgg tggagcatca atacctgatt atcaaacagc aagtatagta
     4441 gccacattta taactattac gactaagcaa gaagcatctg taggaatctc tataggtatt
     4501 ccagttgctt tattgatggt acaattagat gttcttagaa atactattgg tatatggcta
     4561 gttcataaag cagaagatgg agctaaaaaa ggaaattata aaaatataac ctatatgcaa
     4621 atgttaggag tactattaac agcagctaca acaggtattc cagttgctct atctgtaata
     4681 tttggacctt ctttaattaa taccattttg aaatatacac ctgaatggtt aacaggtggt
     4741 ttaacagtag ctggaggact acttccagca gtagggatag gattattact tagatatctt
     4801 cctgctaagg agtattttag ttacttagta attggttttg ttttagcagt atatatgaaa
     4861 gtaccattat taggtgtagc tttaattggt ggagcaattg cacttataat ttacaaaaag
     4921 aatttggaaa atcaagaaca acaatatact gtagtaggag gtatggacga agatgagtga
     4981 taaggtatca ttaaataaat caaatgaaaa ggtgaaaaaa agtgtcttat ggcgctggtt
     5041 ttttactagt tctgtttctt caaattatga gaagatgcag gccttggcat actgttatgc
     5101 agtactacct tttttaaaag tagtatacaa agataatcca gaaaagttac aaagtgctgt
     5161 attaaatcat ttacaatttt ttaacactaa tccttgggtt gccccttata ttttgggtat
     5221 taatgtagca atggaggagt tatcaagtga agagactgaa gaggctgttg cttcaattaa
     5281 aacaggcttg atgggaccag tagcaggttt aggagatagt ttatttgtcg ttattccgtg
     5341 gacaattttt ggagctatag cagctaatat ggcgatagat ggaagtccat ttggaatctt
     5401 actgtggatt gtagtgagtg tagcattgaa attattaagt ataccactat ttaatgctgg
     5461 ctatacatca ggtactaagc taatatcttc aattgaaaaa agtttaaaga ttttaactga
     5521 gagtacatct attttaggtt taatggttgt aggtgcatta ataccttcag ttgtaaaagc
     5581 taatgttgct ttgaactttc aacaaggtga ttttacaatg aaaggtcaag aaatattaga
     5641 ccaaattatg cctgggttag ttcctgcaat tttagttgtg attgtttatt gggctcttaa
     5701 aaagaatata aaacctatat acttaatttt aggagtaatg gttgtttcaa taattttata
     5761 tgcattaggt attttaaaat aaggaaagga tgatattaga tatggaaatg aaaggtatta
     5821 aaaatattcg tatagatgac cgtttaatcc atggtcaagt tgcaactatg tggagtaata
     5881 aattgggtgt tacaagatta atggtagtaa atgatgcagt agctaacaat tctgttcaaa
     5941 aacaagtttt aagaatggct acacctgcag gtatagcgtc atctataatt acagaagaga
     6001 ctgctataaa aaatattact gcaggaaagt atgaaggaca aaatgtatta ttaattgtta
     6061 agtctcctgt ggaccttatt ccatttgtag aggcaggatt taaaatacat aatataaatg
     6121 taggaaatat gtcaagtaga aaagatacaa cagttctaag acctaatatc agtgttactg
     6181 aagaagagaa agaagcattt aaaagacttt tagatgaagg aattgaaatt acaactataa
     6241 tgacgcctga tgataagaaa acttatcttg tagacatctt atagatttaa agaggtgtat
     6301 taaagtgaat agtattattt tgatgagtca tggcaatatg gctgaagaag tcttaaattc
     6361 tgcaaaaatg ataatagggg attctataga ttatcctact gtaaatatga aacaagatga
     6421 tggaatagaa ggaacaatag aaaaattaaa aaaagcttta gaagtgtaca aggaaagttc
     6481 agaaataatt ataatggcag atttactagg tggaactcca tgcaatgctg ctttattagt
     6541 agccaatgat gacagtcgaa ttaaaattat ttcagggcta aatttaggta tggttattga
     6601 atcagctttt ttagatacaa aggaattggc agcaaaatta gagaaaatag gtaaagataa
     6661 tatttctatg gttgaacaaa atgcaagttt attatctgat gatgagtagg aagattatat
     6721 taatatttaa aacaaaatac ttgtgttaat acttaattga tatacaattt ttaaaaaata
     6781 tagctaatct tgcataaact atataataaa tctaatttta gaatgtataa tttaaagggt
     6841 tatggctgta tatagtcata acccttatat ttcattaatt aaagttaaat tttgtatatt
     6901 caaaaagctc ttgatattta cggcagagcc accatataga gatgcctcag accctaatga
     6961 tgaaatttct atatttattc ctttgttaaa agagctaact aacatatctt ttgtaatctg
     7021 taatatctca gggatatcac taattatttg gctatttaaa tatattattt caggagcata
     7081 agttgtaata gcattattta tagctattgt taagtaacta caaaactcat gaataacttt
     7141 tttggcattc tggttatctt cataatatag ttgctttact atatcagaat ctattttagg
     7201 aatattttct aaagaagaaa gctgttcaaa tacctttttt tctgaacaat actgttctaa
     7261 acagccacga tttccacaag gacatagttt accattaggc atgatgatag tgtgaccaat
     7321 ttctccactc ataccatttc tgccgctata taatttatta tttattataa tgcctgaacc
     7381 aaatccactg tgaatactga gactaagtag tgagttatgt acagttgaaa aagtattctc
     7441 agctaaagct gttaagtttg cttcattttc tatatgaata ggaaagtcat actttttgct
     7501 aaggatgcta tataagtcaa tctcattaag attataatat ggagtaaaca atactttatt
     7561 ttcacaagta atcccatgta tagccaaagt aagaccaatt accttataag gagtgtctat
     7621 tttggatatg ttataactat ttataatttc atctatcaat tgtataacat tgtctttact
     7681 tacttgtata tctgttaatt tcttagaatt tataatagtt ccatctaagt aagatagaga
     7741 agaaaatata tagtcgtatc caatgtccat acttaaagaa atccctgcac atttattaaa
     7801 tactaataat atgggttttc ttccgccact atgagtacta tttccaattc ctatttcatg
     7861 aactagagat tcatcaataa gttttttggt aatagcggat atagttgctt tattcaatcc
     7921 tatagtagaa gctatacttg ccctagaaat aggaccattt tttataattt gttcaagtac
     7981 caatctttca ttcatttctc gaatagtgta tttatcagta accaatttga tattcctcct
     8041 taaaataata ttgtaatact ttttacacaa aaataaaagg ttattttgca ttgacaaaga
     8101 taattaaata ttttattatt agttcataag ttagtttaat atactaacaa aaataaagca
     8161 agtaaaatat acctaaaata taaaaaaatt aggataggaa aacgatagtt atgaagtggc
     8221 attcaaggag ggatgaagtt ggagtatgta ttaggtgtgg atattggtac atcaggtact
     8281 aaaacagttt tatttgataa actaggaaat attataaaaa gttgtacata tgaatatcct
     8341 cttatacaag agaaatcagg ttgggcagag caggatgcaa atgattggtg gaaagctgta
     8401 gtcgaatcta taagagaagt agttcaaagt tcaaatataa gcagtgaatg tataaaagga
     8461 attggattat cagggcagat gcatggtcta gttatgctgg acaatgaagg taaaacattg
     8521 agaaactcta taatatggtg tgaccaacga acagtaaaag agtgtgagga gataactgat
     8581 ttagtaggtg aagaaagact gattgaaata actgctaatc cagcgcttac aggatttaca
     8641 gcatcaaaga tattatgggt tagaaacaat gaaccagata tatatataaa tactaataaa
     8701 atacttcttc caaaagatta tataagatac aaattgacag gtgaatatgc tacagaggta
     8761 tcagatgcaa gtggtatgca gttgctcgat ataagaaaaa gagattggag cgatgaagta
     8821 ctagaaaagt taaatataga taaaaatctt ttaggtaaag tttatgaatc acaagagatt
     8881 acaggttatg tgactagaga tgtagcaagt ttgacagggc ttaaagaagg aacaatagtg
     8941 gtaggaggag ctggagacca agcagcagga gctataggaa atggaatagt taaagatgga
     9001 gtagtttcat ctactatagg tacatcaggt gtagtatttg cttatacgaa agagccaaaa
     9061 attgataaag aaggtagagt tcatactttc tgtcatgcaa taccaaatac atggcatgtt
     9121 atgggagtta ctcaaggagc tggattgtca ttaaaatggt ttaaagataa tttctgtcaa
     9181 agtgaagtgg aagtttcaaa ttctttaggt gaagatgtat atgagattat aaataatcaa
     9241 gtaagtcaag taccaacagg atgtaatgga ttgctttact taccatatat gatgggagaa
     9301 agaactcctc atcttgaccc ttatgcaaga ggtgtgttct ttggattatc tccaatccat
     9361 tctaaaaaag aaatcgcaag gtcaataatg gaaggtgttt catatagtct taaagactgt
     9421 atggatataa tagagaatct taatatagac gttaatgaag taagagcctc aggtggaggt
     9481 ggaaaaagta aagtatggag acagatgcaa gcagatatgt ttaatcaaga tgtatatact
     9541 ataaattcaa gtgaaggacc agcacttgga gttgcaattc ttgcattagt aggagctgga
     9601 atatatgaaa atatacaaaa agcttgtgat gctataataa aaacttcaac taaattagaa
     9661 ccaataagtg aaaatgtaga tacatacaag atatatcata gattgtataa aagaatatac
     9721 aaatctttga aagatgactt taagttatta gatgaagtag ttaatacaaa aataaataat
     9781 taaattagct taaaaagaaa aataaagata gaaaatagtt ttaaattaag aaaacatacg
     9841 acatatatta tatgtgtaaa atattaggag gacatgaaat gagtgaaata tttaaaggaa
     9901 taggacagat aaaatttgaa ggagtaaaat ctgacaatga attagcattt agatactaca
     9961 atcctgaaca agtggtaggt aataagacta tgaaagaaca cttaagattt gcaatgagct
    10021 attggcatac tttatgtgga gagggtaatg acccatttgg agtaggcaca gtagagagac
    10081 cttggaataa tgtaacagac ccaattgaga tagcaaagat taaagttgat gcaggatttg
    10141 aatttatgtc taagatggga attgagtatt tctgttttca tgacagagat atagctccag
    10201 aaggaagaga tttagaagag acaaataaga tattagatga aatagttgag tatataaaag
    10261 ttaatatgga aaagacaggt ataaagttat tatggggaac tgccaatatg tttggaaatc
    10321 caagatttgt tcatggtgca tctacaacat gtaatgcaga tgtatatgca tatgcagcag
    10381 ctcaggttaa aaaagctatg gaaataacta agtacttagg aggagaaaat tttgtattct
    10441 ggggtggaag agaaggttat gaaactttac ttaacactaa tacagaactt gaaatggata
    10501 actttgcaag attcttacaa atggctgtag attatgctaa ggaaatagga ttcactggcc
    10561 aattcttaat agagcctaag ccaaaagagc ctactaaaca tcaatacgat tttgatactg
    10621 ctactgtttt aggattttta agaaagtata atctggataa atacttcaaa atgaatatag
    10681 aagcaaacca tgcaacactt gcaggacata ctttccaaca tgaattaaat atagcaagaa
    10741 taaacaatgt tttaggtagt atagatgcaa atcaaggaga cctattatta ggttgggata
    10801 cagaccaatt tccaacaaat atatatgatg caacacttgc tatgtatgaa gtgttaaagc
    10861 aaggtggaat agcaccagga ggatttaact ttgactctaa agtaagaaga gcatcttttg
    10921 aggtagaaga cttattccta gcttatattg caggaatgga tacatttgca aagggattat
    10981 taatagctca taaattatta gaagatgagg tatttgaaaa ctttactaaa gaaagatatg
    11041 caagttttag tgaaggtata ggtaaggata tagtagaagg taaggttgga tttaaagagt
    11101 tagaatctta tgcactgcaa atgccagtta taaagaataa gtctggaaga caagaaatgc
    11161 tagaagctat acttaacaga tatatatatg aagtagatac aatttctaat aagtaattgt
    11221 gtagtaatta aacttggatg tttgaataaa ataacaggat atgttcttgt ttttattcta
    11281 aaacggacaa agataagtat ttgtaaaaat tctcataatc atgataaatt aaggatatag
    11341 acttaagtat aattagttta tatccttaaa atttctataa aaaataatca tttctatagt
    11401 tatcaatatt tttaattatc aatacctttc tcaactaata aataaaaatc agtaaagtat
    11461 ctaaatttaa gtcattataa tggataaatt ttttagcata tcttttttag tatatcactg
    11521 ataagaattt actttcagga tatagcagta aaatagatat ttgaatataa ttgttatttt
    11581 ataactttca tgaaataatt ttgaaaatac aaataaaaat ttttaaatag aataatagtg
    11641 gaatatatct tcaagaattt cttttaactg tttaggtgat aataaaataa tgatacaatg
    11701 tattaaaata taaaatctga aagtggatga gaattacatg aaaaagaaag tattattttt
    11761 agtaattatt ttagcattta taatcttatt aggtaattct acttattctc aaaagaatca
    11821 gttgtctgaa gaggaaaagg tggatgactt taattatgtg tataatgttg taaagacagg
    11881 atatccatat ctaggtgtaa ataagagact taataatatt gattggttag caaataagga
    11941 tgaatataca aaacgaataa aaaacactga aaatgatgaa gaatttatag aagaactgtc
    12001 atctatatta tctgatttaa ataataaaca cacagaagta atagataata agaaaagata
    12061 tgaacttttt aagaaatcat actctaataa tgattggtat gattttctta atgataaaaa
    12121 agttgtggat agatatgact ctatgaatct taaaataaaa atgccagatg atatttttgt
    12181 aaaaaaagaa ttaattttaa aggatgttat taagggtcag attggatata tgtatttacc
    12241 atcaatgtca tcaaagaatg gctctataaa taatgattta aaaataattg gagattatat
    12301 taacacttta gaaaaacata aagctttaat tattgatata agaggtaatt taggtggaag
    12361 tgataggtat tggcagggaa tcgtatctaa actcataaaa aatgatgtca aaataaatgg
    12421 ttatagaatt tatagaaata ataatgaaat agtaaaaaaa tatacaaatg caagaaatat
    12481 aaaattaaat cctgtagaaa atttaccagc aagtgtaaag gaaaatgcac ctaaagaaat
    12541 tataaaagat tttaatgatt ttgaagatac ttcctatact ataaaatcta cagatgattc
    12601 aaaatttaag ggtaatatat acttacttgt tgatagaaaa atatattcat catcagagtc
    12661 atttgcaatg ttttgtaaag aaactaaatt tgcgacttta gttggtgaaa ctactggcgg
    12721 agatggaggg tgtatagacc ctgtattgtt taatttaaaa aatagtggtc taatagttag
    12781 aatggcaagt tgtatgtatt taaataagaa tggaatatgt gatgaggaat ttaaaactat
    12841 tccagaattt aaagtaaaag attgtaaaag gactgctgat tttaaagatg ataattgcat
    12901 aagaaaagca ctagaacttg aaaatattaa ttattagata aaagaataaa attaaaacta
    12961 cattttttta aacatcaatt atagcagtat tttttatagt caattttatg ggctaaatag
    13021 taaatataag aatacgctgt aatttaatgt tgaatattgg tataatattt atgacgattt
    13081 ttaattaaag gggataatag tttatgagat tagaagaaat gattttttta aattataata
    13141 aattgaatga tacagattta ttaatatgga aatatataac tgcaaataaa aaacattgta
    13201 gtaagatgtc aataaatgaa cttgctatga aatgtaatgt gtctcgtgca actattacaa
    13261 gatttgttaa aaaattagat ttaaaaggtt tcagtgagtt taaagtatta ttaagctggg
    13321 aatcagaaaa ggttcatgtc atagaaaatc agtcatttga tattgcatgt aattcaataa
    13381 ttaagtatgt tgaagatcaa aaaaataaag attatgatgc attgtgtaaa cttctttatg
    13441 aatcaagaac aatatacgct tatggttcag gtgatattca aaatgtggtt gcaaaacaga
    13501 taaagagaat gttcttatca tgtcaagaaa taatatatga ttttgaggga agaacatttg
    13561 atgattcttt ttacaatatt gtcagtaaaa atgatgttat gattattata tccctaagtg
    13621 gtgagaatga gaatacactt aatattgcta gaagattaaa attaatagga actaaaataa
    13681 tttcaattac agagtttaaa aataatacat tgactgattt aagtgatgaa tcaatttata
    13741 tttcagcgac taatatctca tttttacaaa tgcaccctag ttataaattg acaatgttat
    13801 attttatatt gattgagtta ttatttatta aatatagtat ttataaaagg catagaatga
    13861 ttacagaagg gattgattgc aaattgtagt cattcttttt tttatgagaa tttgaacata
    13921 aaaatgtgca aaaacttatt tgactgtgaa taagctccaa atgtacccta aagattgtat
    13981 atatttatta acctgttaaa ataatagata tctaaggcca aagataaact aaaaatatga
    14041 ggaggggagt aatttatatg atgaagaaaa tacagcgttt tggaggggca atgtttacac
    14101 caacactatt atttgctttt gccgggataa tggttggatt ctcaattgta tttcaaaatc
    14161 agtcaattat gggttcactt gctacaccag aaaatatttg gtaccaattt tggggtgtaa
    14221 tatctagtgg agcttggatg gtatttaatc aattaccatt attatttgct atttctttac
    14281 caatagcact tgcaaaaaag caacaagcaa gagcttgtat ggaggcttta gcaacgtatt
    14341 tgacttttaa ttattttgta ggttctatgt taagtttttg gggaaaaagc tttggagttg
    14401 atttcgcagc tgaaataagt gctggaagtg gacttgtaag cattgctggt attaaaacat
    14461 tagatactgg tatggttggt gctttgttaa tatcaggtat tgtcatttat attcataata
    14521 gattttatga taaagaacta ccagatttta ttggattatt tagaggctca tcacttgtag
    14581 ttgcaatttg tttctttgtt atgataccag ttgcattatt aacttgtttt atttggcctc
    14641 atattcaaaa tgttattaga catttgcaaa cgttttttat aaatagtgga aatataggag
    14701 tttggtgtta tgcattctta caaaagattt taataccaac tggattacat cattttgtat
    14761 atgcaccaat ttgttatgat agtgttgttg ttccaggtgg tacatcagta tattgggcta
    14821 ctcatattca ggactttcaa acttcagcta agactctaaa ggaaatgtat ccaatcggat
    14881 tttcattatc tggattatcg aaggtgtttg gttcattagg tgtatttggt gctttctatg
    14941 taactgcaaa gccagaaaaa aagaaaaaag tattaggttt aatgattcct gctactctta
    15001 ctgcagtatt aactggaatt actgagccgt tagaatttac atttttattt gtagcaccac
    15061 ttttattcct agtacatgca tttttagatg catgcttgca aacgatttcg tttgccttag
    15121 gagttgttgg tgattttggt ggaggaatta ttaactgggt tgtattaaat tggcttcctc
    15181 ttggaatgta tcactggaag gtatatgttg ttcaagttgt tgtagggatt atattttcat
    15241 ttatctggtt ctttttattt actttcttaa ttaagaagtt tgatatgaaa actcctggta
    15301 gagaggaaga taatgaagaa acaaaactat atactaaaaa tgaatactta gaaactaaag
    15361 atgaaaaagg aaataaattg agtaaagcct ctcaacaagc atctgaatac ataaaattag
    15421 ttggtggagc agaaaatgtt gtagatgtta caaactgtgc tacgaggttg agattaacct
    15481 tgaaggatga ctcaattatt tctaaagaag aagattttaa ggtagtaggt gcacatggtt
    15541 tagtgcataa tggaaaagct attcagatta ttattgggct ttcagtacca agtgtaagag
    15601 aagaatttga aaatttatta taaaggagaa ggaaagatgg aaagaaaaag acagaggata
    15661 ctaattgctg gtgggggtag tacttatact gctggaattg taacaatgtt aattgaaagt
    15721 gttgctaaat ttcctattga atcaattaaa ttatatgata ataatgatga gagacaaaga
    15781 aaagttgctg aagcatgtgc aataattgtt agagagaaaa atccagaaat taaatttagt
    15841 tatacaacaa atccagaaga agcatttact gatattgatt ttgtaatggc acaaattcgt
    15901 gttggtttat atgctttacg tgaacaagat gaaaaaattc cattaaaata tggggttgtt
    15961 ggtcaagaaa cttgtggggc aggtggaatt gcctatggat taagaacaat aggtccaata
    16021 atcgaaatga ttgattatat ggaaaaatat tctccaaatg cttggatgct taattattca
    16081 aatccagcag ctatagttgc agaagcatgt cgtgtattaa gaccaaattc aagaattatt
    16141 aatatttgtg atatgccagt atgtttagag gaaatatttg ctagaatttt aggtttaaat
    16201 tctcgtaaag actttgatgt tagatattat ggattgaatc atttcgggtg gtggacaagt
    16261 attaaggaca aagaaggaaa tgatttaatg cctaaattac aggaatattg tgctaaaaaa
    16321 ggatatgagg aatttacacc tcaaggtcaa cacaaagaat ctagttggtt agaaactatg
    16381 agagcagcaa aggacttatt agaaatagaa ccgactacat tacctaatac ttatttaaaa
    16441 tattatttaa tggctgatga aactgttgaa catgctaatc caaattatac aagagcaaat
    16501 gaaattatgg atagaagaga aaaggataca ttcgaagaat gtgaaagaat tataaaaaat
    16561 ggcacagcaa gggatacatg gtttgatgca agtgaacatt ctcaatttat tgttgaatta
    16621 gcttgtgcat tagcatttaa tactcaggaa agatttttac tgattgttcc taataatgga
    16681 gctattgaaa attttgcaga tgatgcaatg attgaaattc cttgccttgt tggaaaagat
    16741 atggttgaac ctatgtctat aggtaaaatt ccaactttcc aaaaaggttt aatggaacaa
    16801 caagttgctt ctgaaaaatt agctgtagaa gcatggatag aaaaaagtta tcaaaaatta
    16861 tggcagtcgt ttactatgag taaaactgtt ccaagtgcaa aagtagcaaa agaaatctta
    16921 gatgaaatga ttgtggctaa taaggatttc tggccaactt ttaaataagg agaacaaaaa
    16981 catgataaca aataaaataa aaaatataac agcagtatta aaagatgaaa atatatttct
    17041 ttcagtggtt atggtttgtt ttctaatatt acctgtttta tatatagtgt atttagaatt
    17101 gacatcatta tttacaaaca tttcattttc acaactttta aacaatgatg ttaatgcaag
    17161 tttaaattta atctctaact ttagtgcagt atacagtgca tatgttattt acaatataaa
    17221 aaagaataaa gagataaaag aaaacatgat tgctttctat ttagtcttat tagggcagat
    17281 attccaaatt tacacaataa ctattgttat catgtttacc tatatttata aatttgttgg
    17341 aattaaacaa atcataagca gtattaaaga actacaatgg ggaaagagct ataaggctct
    17401 tttttcctca attgcagtat gcttaatatc gttatttgta atgataatta gaataaagtt
    17461 aattatgtag aggagtgtct ataatgttct ttaaaagaaa aaaagaagca agtacaatta
    17521 attcatacat tgatggaaaa ataattaatc ttggagaaat tcctgataaa gtattttcaa
    17581 ctaaggtaat gggagatgga tttgctatga tagcaaagga caatattctt aaatcaccga
    17641 ctaaaggagt aataagtttt attgctcaaa ctaaccatgc tataggaatt aaattgcaaa
    17701 atgatgttca aattcttatt catattggat tggatagtgc aacaacaaaa aaagaaaact
    17761 ttaaattaga agttaaacaa ggtcaagaag ttaatatggg ggaaaagtta gtagaaattg
    17821 ataataagtt acttgaaaat actttatata ttcctttagt tattgtagag aatcctaata
    17881 cggtaaaata tcatctttca aacttaaatc aggaaggaaa tagtggtatt aaagttatca
    17941 attttgattg attatgatta ataaagcgat taaatttaaa tttatcatat tctatttttt
    18001 ctcatatatt atttttgcct ttgcttcaac aaaattcaca cctttcttat caaaattagg
    18061 ttactcagcg tttgaaagag gaattatatt atcgagctat gcaataacca atatattatt
    18121 tcaattatta tttggagtat tagctgataa gtatcaaaca atgaaaaaaa ttgttttaat
    18181 tagtttatca gtttatggaa ttgcatcagt tattatgttt tctactgaat ctgcaacttt
    18241 tataatatac ttattacttg ttgctttatc aggaggatta ttaaatactt gctgtagttt
    18301 atatgataca tggataatag gttgtggaga taatattaat aattatttgt catttattaa
    18361 gacttttggg tcgattggat gggctattgg aagtatggta gcatcttact tgattcttag
    18421 attctcatat tttggattat ctgtagcgat tggtttaatt gtagtattac ttattataaa
    18481 tatactatta ttacctgata ttgaaaagat taataagaaa ataaatgtaa ctaataaaga
    18541 tattattgaa ttattaaaag ataaaaaata tatcttacta gtatgtattt tattcttatt
    18601 atactcaatg gtggtagcaa ataactgtac tgtaatagat aaaatgatag ctttaaatgc
    18661 tacaaattct cagatatctt taaagtggtc tttacagtca ttattagaga taccaacata
    18721 tctggctggt tcttatttat taaaaaaata tagcagttta aacttattaa aattaagtgc
    18781 tattatgata acagttcagt ttctattatt tgctttaaca aatgattcaa atatcattat
    18841 tatactgtgt gtttttcaag tgttttctac tcctttaatt ttaattactt caaaaagatt
    18901 aatatttgaa gtttctccta aaaaacttag aagtagtagc caattaatag ctttaagtat
    18961 atttatggga ggttcatctc tgattattcc aactgttgct ggttttttga gtattaatat
    19021 tgggtataat tatacattaa tgatattaag tctttttgga tgcttagctt atggcttgat
    19081 agtcttattg aattatcttc aagaataatt gttttaatat atagtagtaa tatcacttgt
    19141 ttatagtacc tgtatttgcc acaatcaagg ttgtattttt ggttttttat agagaagaat
    19201 tattgtatag attagaggct tcttaggggc ttcttttctt tgtttataca aaaaagtgaa
    19261 tcatgctttt attttaagat atttccacta taattataat agtcttccat atgttacaaa
    19321 agtgtaatgc taacagttaa aatgtaatat ttacttaagg atttatttat ataaaacctt
    19381 taaaataaat atatattaaa aaattagcaa ctggaggcta ttattatgaa agaaatatta
    19441 aaaataaaaa atatatcaaa agattatgga ataaaaggat ttaaaacaaa tgtgttaaaa
    19501 aatatatctc ttacagtcaa tgaaggtgat tttatagcaa taatgggtcc atctggtgca
    19561 ggaaagacaa cattgctaaa tttaatgtct acacttgaca aacaaacttc aggagaaata
    19621 atactagatg gaattaatat atcaaaagta aaaaataatg aactatcaaa attaagaaga
    19681 gaaaaaatag ggtttatatt tcaagactat aatctattag acaatatgaa actaatggac
    19741 aatatagcac ttcctctagc attaggaaaa aagaaaagca aggagataga agctaaggta
    19801 ttttctatag ctaaaaaatt tggattagaa aatcatttag ataaatatcc atatcagtta
    19861 tcaggaggac aaaaacaaag aggcgcagca gctaggtcat taattacaaa tccagcagtc
    19921 atatttgctg atgaaccaac aggtgctttg gactctaaat cagcatatga acttttagaa
    19981 tcattagaaa aaataaacag agaaaacaac gctactatta taatgattac acatgaccct
    20041 ctaacggcaa gttattcaaa tgaagtatat atgataaatg atggaaatat aaagtgtaaa
    20101 ttaaacaaag gaaatagtag aaaagaattt tatggaaaaa ttatggatat gctagcctct
    20161 atgggaggtg agatgtaagt gagtatactt ggaattgctt ataataattt taagagtaac
    20221 ataagaactt atctagcatt ttttatatct atggtttttt cagtagttgt gctgactaat
    20281 tttgaattat taagatatgg agatgctata aatgtgttac aagaggaaaa taaaaaattt
    20341 acactatcag tgttagtagc tgtaataatg atactatcag tatttttatt tttctttata
    20401 tggtatgcaa caaatatatt ttttaagaat aggtctaaag aaattggaat attgtccttt
    20461 atgggattag atttatatac tataggaaaa atatattttg tagaaaatat gcttatagga
    20521 ataagttctt gtataacagg aataattata gggataataa catctagatt ttttcaagta
    20581 gtgataataa aattatctgg atttaatata aaggtatcta atggacttag catagaagct
    20641 atactatatg ctactttaat ctttatttct atatttataa taatggctgt aaaaggtttt
    20701 ataacaatat gtagaagtag tgtaataaat ctaatcaata tatctaaaaa acaagaaaaa
    20761 ataccaaagg taggaatagg tatatatata ttagctatag tatcacttat agtaatagga
    20821 tatggatact atctttctac gaatataaga actggagata taactagtgt actacctgtt
    20881 ataggtataa taatagtagg tacttatggc ttattcaaat cagttatgcc tgttatattt
    20941 gatattataa tcaaaaataa aaaaatatta tttaatggaa ataatataat tgcgataaac
    21001 aatataaact accgattaaa taaaaactat aaaacttatg ctataatagc tataattata
    21061 actacaacaa tatcaactct tggtgcttca gttgctatga agcatataca taagaatgcc
    21121 caagaacaaa gaaatgtgta cactattagc atagtttcaa tggataaaga tgaagtgaat
    21181 aaaaaacaaa tagagaacat aataaaagat actaataaga taaagtactc cgtaaatcca
    21241 gaactaacaa tcttaaagaa aacagatata aaagaagctt atgatacaac agatactata
    21301 atgaaatata gtgatttttg taatactctt aaagtgaatg gcaatgataa agaattaaaa
    21361 gagtatggtg aaagattagt tagtggaaat aatgtaatag agctaaaaag tcctcaaacg
    21421 atagcatcaa tagctacaaa agaagtcaag acaaaacttg atggtcagtt atataatatt
    21481 tcaaggggag aagtaaaagt tccagtatta gggacaggac ttaatcaaag tattatagtg
    21541 gtaaatgatg atacatattt taaactaaga gataagggtc aacaagtata tttttatggt
    21601 gcaaaggtat ttcaagaaga aaatagccaa gtaatgttca acaatattga aaagaattta
    21661 agtttaaaaa atggatatat tcagaatgga tataaagctc aaggtgagag tcaatggatg
    21721 aagtttgcat atgtagtgct tgtgttttta ttcttagtct ttgcaattgt agctggaagt
    21781 ataatatata tgaaaatata tagtgatgca tatgaagata aagagaaata caagatttta
    21841 ttaaaaatag gtactacaga aaaagatata aatagagcta tacttaaaga agtagctata
    21901 ttttatactt tgccaatgtt aagtgcaact atatctagtt attttgcatt gagattagca
    21961 ggagatttat tgatgaacga tttatctggt atatatatac taagtttagt gatatgtttg
    22021 ataatattcg taatatatgg agcagtatct gttaataagt ttaagagtgt agtgtataaa
    22081 aattagttga tatattaata aagagaaagg agattcttgt aataagagtc tccttgttga
    22141 ttaactaaat aatttgggta ataaatttat ataaaatatg ttatgatatt aataattcat
    22201 ggcatagtga aatattagga taatggagaa tatacaaatg tatagaattt tattagtaga
    22261 ggacgatatt gatttatcaa aagagattgc gttagcactt gaaaaatggg gatttaaagt
    22321 agaattaatt gatgattttg aggtagtatt agatgaattt atggataaaa aaccagatgt
    22381 agtactttta gatgtaaatt taccattata taatggattt tactggtgcg aaaaaataag
    22441 agctatttca aatgtaccat taatattttt atcatcaaga gattctgata tggatttaat
    22501 aatgggtata aataatggag cagatgatta tattaccaaa ccattttcta tagacatatt
    22561 ggttaccaaa ataaatggta taataagaag agtctataat tatagtgatt ctaatagtat
    22621 tttgtattgt gaagatttga tgtttgatgt ggggaaaggg attataaaac ataagtataa
    22681 ggataaaagt atagagttga ctaaaaacga aataaaaatt ttaacattat tattaaaaaa
    22741 taagaatagg gttgtatcaa gggaaagtct tatgatgact ctatgggata atgatgagtt
    22801 tgtgacagac aatgcactta ctgtaaatat gaatagattg agaagtaaag ttaaagaact
    22861 aggttttgat gattttataa aaactaagaa ggggatagga tatataatat aatgctagta
    22921 aaatatttaa aaggtaggtc taagtatatt gctctacctt tagttgtagt tagtataata
    22981 aatatctatt tatttgctat agatatttta aaaaatagat attcagaatt aatatactta
    23041 gattttttag tg
//
LOCUS       DAMKPG010000061        22737 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMKPG010000061 DAMKPG010000000
VERSION     DAMKPG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.064"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            83..1519
                     /locus_tag="PC621_002419"
     CDS             83..1519
                     /locus_tag="PC621_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF4109566.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            complement(1611..3485)
                     /gene="asnB"
                     /locus_tag="PC621_002420"
     CDS             complement(1611..3485)
                     /gene="asnB"
                     /locus_tag="PC621_002420"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF4109567.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3688..4362
                     /locus_tag="PC621_002421"
     CDS             3688..4362
                     /locus_tag="PC621_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF4109568.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4475..5011
                     /locus_tag="PC621_002422"
     CDS             4475..5011
                     /locus_tag="PC621_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF4109569.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5177..5797)
                     /locus_tag="PC621_002423"
     CDS             complement(5177..5797)
                     /locus_tag="PC621_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF4109570.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6111..8249)
                     /gene="vanT"
                     /locus_tag="PC621_002424"
     CDS             complement(6111..8249)
                     /gene="vanT"
                     /locus_tag="PC621_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF4109571.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8267..9073)
                     /locus_tag="PC621_002425"
     CDS             complement(8267..9073)
                     /locus_tag="PC621_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF4109572.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9070..10170)
                     /gene="vanG"
                     /locus_tag="PC621_002426"
     CDS             complement(9070..10170)
                     /gene="vanG"
                     /locus_tag="PC621_002426"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF4109573.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10431..11513)
                     /locus_tag="PC621_002427"
     CDS             complement(10431..11513)
                     /locus_tag="PC621_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4109574.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(11563..12264)
                     /gene="vanR"
                     /locus_tag="PC621_002428"
     CDS             complement(11563..12264)
                     /gene="vanR"
                     /locus_tag="PC621_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF4109575.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKAQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12698..15229)
                     /locus_tag="PC621_002429"
     CDS             complement(12698..15229)
                     /locus_tag="PC621_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF4109576.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15337..15954)
                     /locus_tag="PC621_002430"
     CDS             complement(15337..15954)
                     /locus_tag="PC621_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF4109577.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16316..17200)
                     /locus_tag="PC621_002431"
     CDS             complement(16316..17200)
                     /locus_tag="PC621_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF4109578.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17391..18641)
                     /locus_tag="PC621_002432"
     CDS             complement(17391..18641)
                     /locus_tag="PC621_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4109579.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(18863..19627)
                     /locus_tag="PC621_002433"
     CDS             complement(18863..19627)
                     /locus_tag="PC621_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109580.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19621..20346)
                     /locus_tag="PC621_002434"
     CDS             complement(19621..20346)
                     /locus_tag="PC621_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109581.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20348..20716)
                     /locus_tag="PC621_002435"
     CDS             complement(20348..20716)
                     /locus_tag="PC621_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4109582.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20892..22436)
                     /locus_tag="PC621_002436"
     CDS             complement(20892..22436)
                     /locus_tag="PC621_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF4109583.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7846 a         3819 c         2368 g         8704 t
ORIGIN      
        1 tttgaaacta caaaattatt gtactatatt gttttaagtt gttttataat agattatatc
       61 atcttattat aggagaaaaa atatgatttg tactattaaa ccattaaaga tttcaataaa
      121 aaagtcaact gaacctctaa ttgaatttat atctgatact acctataaaa ataagagttc
      181 ttcatatatt aaagaagtcc actttacaag atatataggt gttgatgatt caattgataa
      241 atacatagaa aaagttaaaa atatagatac ttattttttt aataatccta caattcccta
      301 tattagatta aacaatctaa aaataaattt tgacaaagaa caaacagata agatgcttaa
      361 aatttttaat atttatgata gttctaagtt taacagttgg gatttatata atatcgactt
      421 tccatgtaaa atagaaaatg acactttaaa ttggactaaa aagattgcat ttaaaaatac
      481 tttagattta ttttccacaa gtatgccaaa ttgtacatcc tctatcatca aaaactttgc
      541 agttaaactt ttgtgttgga tggactattt tctacctaaa ttattttata acaaggtgaa
      601 gactactatt tcaccaaaga tagtatactt tggaaatatt aaaaggcaag aacttttttt
      661 cttatatttt ttatcacaac ttgggtgtga tattttatat ataaatccaa ataaagatat
      721 tcttgattta tatcctgaat gttcaaaatt ttctactcta tttgaacttt ctaggaaaac
      781 tccaaatata ttagaaatac cttttgaaag gcatattaca aatataaatt acagtgaaaa
      841 taccaataag acttctccta ctacgaataa acctaactct aaaaatacac ttaataacac
      901 aaatctaaca tctaaaaaaa ttgcacaaga tgaggaagta gaactttctt atgaagacct
      961 tgcaaagtta tcagcttctg ttgttatgat tgttgtgtgc aataacgaaa ataaaccgtt
     1021 taagagtggt tctggagtag taataaataa tgaaggttat atacttacga atcttcatgt
     1081 agtcaatgat gggtattcat ttttagttag atttgaaaat gatgataaag tttatacatc
     1141 ttaccaaatt ataaaatacc attcagatta tgatttagct gtgataaagg ttgatagaaa
     1201 atgcaaacca atacctgtta aagtcagtaa aaaacctgtg agaggtcaaa aaatagttgc
     1261 aattggaagc cctttaggtc tttttaatac agtttcagat ggaattatct cagcttttcg
     1321 tgattttgaa acggttcaaa tgatacaatt tacagctcca atctctagtg gtagttcagg
     1381 aggagctctt ttagatatgt ttggaaactt actaggattg atttccgctg gatatgatga
     1441 tggacaaaac ttaaatttag ctgttgaaag taatcttgtc aaaatatttg ccaataattt
     1501 tattgagata gtaaactaat caatcaaaat tctacttatc ctattccata ataatcaaga
     1561 gggtgaaaac attgcatgct tttgccctct gattaatatt gaaaaaagta ttaatactcg
     1621 acatttttat gctcaataaa taattgatgc catagacaaa atgtaaatat agtccatatt
     1681 tttctcgaat tatctctatg acctttaaga tgcgtatcta aaagttttat tgcatatttt
     1741 ttatctataa attcatctac atttgaatta taaatagttt cctttacaat atcccctaga
     1801 gaatctttta accaaactct tattggagtt ggaaatccta attttttcct ttgaacaaca
     1861 tattctggga ctatttctct aaacgcctct ctaagtaaaa cttttgtatt attcttattt
     1921 attttttgct ccattcttaa attttttgct acatcaaata catctttata taaaaatggt
     1981 actctaagtt caacctgctc agctgtagaa agtttgctaa ttttttgaag tatatcgcct
     2041 tgaagccatg tattcacatc tatatgttgc attttactta tataatcgta attatttttt
     2101 tgtgcatctc tatacaaatt agacaacagg tattcatata tattcttttc cttatactta
     2161 aaaaagaatc tttttacttc gctgttttca aaaacttttg catttcctat atatcttttt
     2221 tctaatggtg ttgtagctct ataaagataa tttcttcctt ttatgtttgg cataagttct
     2281 gaaactcttc ctaatatact tttaatataa gtaggtgaat tgacaactga tttcatagta
     2341 ctatactctt tatatatatt atatcctcca aataattcat ctgctccttc tccagacaat
     2401 gctactttaa catgtttacc tgcctcttta attaagaaat aaagacctac ttctgaaggg
     2461 tctgctatag ggtcatccaa agaataaatt acattcggta atgattttat atattcatct
     2521 tgtgttatat taatctggat attttctata cctaacttat cagctgtttt ctttgctact
     2581 tctaattcac tatatccttt gacactaaag cctgcagaaa aagattttat atttggatta
     2641 atttgtgaag ccacagtagt tataatagat gaatctattc caccagacaa aaatgttcca
     2701 atttctttct cctcttccat atgagttact attgaatcta ctactatatt tcttatatca
     2761 tcctcattca catctctatt gcctaagtga tttttagtat aaaaaaattt tgtactactt
     2821 ctaaactcaa gtttattata tctctttaaa gatagtatac cattttctac cctaaaatag
     2881 tgacctgctg gtatcagcct tattccttgt atcatagtat cttcaggaag tacatattga
     2941 aatgaaaaat aactttgtaa gcttctttca tttatattta aatttttcat atattcaagt
     3001 aaaaacttat actctgagga aaaaattatt gcattttcct tatttatata ataaagtggt
     3061 tttatcccaa ataaatctct tgctccaaat atactttcat tttctctatc atagattata
     3121 aagttgaaca ttcctttaat cttatgtaca caattcttac caaattctat ataagatgcc
     3181 aataagactt caatatcatc atttgtagta aaattgtgtc cttttttaat taattcttct
     3241 tttatttcat gatagttata tatatttcca ttaaaaacaa taacttttcc atctttttca
     3301 aatggtttat ttccataaat agcatcaatt acattaaatc tttcaaaatc aaaagttttt
     3361 tcctcatcct tatactctat tctactagaa agtcttgtat tatttacaga ttcttttatt
     3421 ttaaatttaa tatcttcttt tttagatata tcacctttat aataaattga aatattgccg
     3481 tccatataat caaaattcct ttctgtcctt tagtcttatt ctatatttca tagttttgtc
     3541 tttggcttta aaacttattc aatttaaaat gaaattgcta ttaaattctt ataaattatc
     3601 tgttaatatt tcatattttt tttatttcta gaaatactat attaggaggt gatttttatg
     3661 aagaaaaaaa tattaatacc agttattatg tctttattta taatctcaca gtgcataact
     3721 tcatttgctt ttacacctga aaataacaaa tttaaggtta aaccattacc ttatgcatat
     3781 gatgcacttg aaccttatat agataaagaa acaatgaaac tgcatcatga taagcattat
     3841 caagcttatg ttgataaatt aaatgctgct cttgaaaaat atcctgagct ttataattat
     3901 tctttatgtg aattattgca aaatttagat tctttaccta aagatattgc tacaactgta
     3961 agaaataatg caggtggagc ttataatcat aaattctttt ttgatataat gacgccagaa
     4021 aaaaccatac cttctgaatc tttaaaagaa gctattgata gagactttgg ttcttttgaa
     4081 aaatttaagc aagagttcca aaaatctgct ttagatgtct ttggttctgg ttgggcttgg
     4141 cttgtagcta ctaaagatgg gaaattatct attatgacta ctccaaatca ggatagccct
     4201 gtaagtaaaa acctaactcc tataatagga cttgatgttt gggagcatgc ttactattta
     4261 aaatatcaaa atagaagaaa tgaatacatt gacaactggt ttaatgtagt aaattggaat
     4321 ggtgctttag aaaattacaa aaatttaaaa tctcaagatt aattaaatat taaaatcctt
     4381 ttgaaaaaaa cttatgtttg ctatataata taataaaaga tagtctaaat ttaccaatat
     4441 aatgtttata gtgacgattc aggaggattt ttatatgata aaagaaacac atgaaagggg
     4501 aggttatata ttcgcacttt tgatactccc ctttataaac aatctgtacc tagttaaata
     4561 cgatattgca tataaaatag tattaattat aatttatatt tattttgcat atttaggttc
     4621 tttatttcca gatatagata tgagaggttc ttacataagt aaaaaattta tgttaatata
     4681 caaattattt ggaagtaggt ttagacatag agggtttact cacagtctaa ttgccttatt
     4741 acttatttat agttttttta aatctctgac aatcttcact aacaataata tagttttctc
     4801 atgtttgtcc agtgggttta taataggata tctatctcac ttgtgtcttg accttattac
     4861 taaagaaggt attgaattgt tttatcccat taccataaac atttctttac tacctataaa
     4921 aactaactca aaaacagaaa aattcatatc taagctatta aatttcatag ttattttttt
     4981 gattggatat caattttata ttttattata gttgtagaaa aaataaataa caatattttg
     5041 aactaagtat aaataagttt tatatatttt agtttacaaa agaactaaaa attttgtacc
     5101 ttaatacaaa aaataaaacg tatcttaaaa taatttagta ctaaatcatt ttaagatacg
     5161 ttttatttcg tacaagctaa atcattttat catctttatc attgtgatgt tttttatgat
     5221 ggaaatcata attatgattt tcaagatttt tatttactct atttccaatc ttccatgaca
     5281 aaaacaccaa taatataaat gatgctatct ttaaaggact tgttataaaa ccttctatat
     5341 cacttgccac gtaacttata actataaaca ttacaaattt tcctgccaac attgctggca
     5401 caaaatcttt taaatctttt ttaccaaaag ctgatgcgat agtaactaaa caaccaggca
     5461 taaatggaca ggcatacgct ataaataata acttaaatcc ctgtttatcc atccaactta
     5521 ttgctttttc agttttacta tttctcaact tattaaaaag cttactatca ttaaatctgc
     5581 ttattaataa gaataatgaa gttgtcccaa gtcctgagcc aatccatgat aacaaacatc
     5641 caataaataa tccttgtatt gcagcatttg ctgtaactat agcaacaagt ggtaaggctg
     5701 gtataaagct ttctataaat gcacttaata agccgattat tgtggctaac acccaataat
     5761 tttgagtcaa atcaagtaat tcttgtatat actgcatatt gcttttcccc ttttttaact
     5821 ttaactatta ttatatactc tatatgttta ttatatacca atctcaaaaa atgaatctaa
     5881 tattaaaaag taaataatct atataagttt acagttaagg tataattatt ttattatata
     5941 ttataacaaa aaaggtgatg tttcaaaata aaaatctgaa acatcacctt tttataactt
     6001 aaattgtcat gtaagttata aaaaatatat attagacata atattactaa aaatgtctag
     6061 tcagagtaaa ataaattaca cagtacaaca aatattacta tttaattgag ttacttcttt
     6121 ataacattta accttcttcc tactctgctc aataattcat tcgatatact acatgagtta
     6181 tttgcaactt cagaagcaga taattcagat aaattatctc ttcctattat tattgccgta
     6241 ttaccaactt ctacattagg tatatctgta atatcaacag caagttggtc catgcaaatt
     6301 cgacccacta ttgctgctct acaaccattt attattacgt aactttttcc acaggataaa
     6361 tttctaggta taccatcggc atagccaata gataatatag caattacgct atctctgctt
     6421 gcaataaaga ctcttccata tccaaaacta tcatcttttt gtatttttct aataagaatt
     6481 acttgtgatt ttaaggataa aacaggtcgt aaatcaagtt gcaatttagt cgtatcattt
     6541 ggagaactta atatcccgta taatgcaatt ccaattctta cataattaca cttaagattg
     6601 ggataattaa aaaaaccata actactttga atatgtactt taggaatttt tatacctttt
     6661 tttaacagaa aatcaattaa cttataaaaa cattcaattt gtttatttgt aaagtctaca
     6721 tcttcatctt tcagactatc tgaaacacac aagtgtgtaa atatgccaca tatatctaag
     6781 tattttaatt taaatgcctt taaaattgac ctcacatcat taacatcaaa acccagtctg
     6841 tgcatacctg tatcaatttt aatgtgtgat tttatgttgt atttctgttt gtttaaagag
     6901 atagcatgat taaggtcaat aacactctgt gtaagattgt atttatgaag ttcctttgcc
     6961 cttattgggt tagtatatcc aagaatcaat atttctccta gtattccata tcgacgtaaa
     7021 tcaatcccct catctattgt tgctactgca aatgctttta cacctatttg gtttgcacaa
     7081 gtcgctactt caaaagcact atgaccatag gcattagctt ttacaactgc catcaattca
     7141 caatcttctg gcatagcttt ttgtatcatc tttacattat gttccaaatt atttaaatca
     7201 atttcaatcc aagctctatt ggtatttacc ttatgttctc ctttcaagtc tttttttaga
     7261 ggtttaagaa tcaatataat aattatagaa gctataaaag atgctatact taccactata
     7321 taatgaataa ggctattgtc aaccaagaca gtttgtattc cgaatatttt agaaaacata
     7381 cgaataacaa caatcatcat aggatgtata atataaataa ttaatgatga tgttcgcaat
     7441 aacctagctc gagaaccttt taaaaatgtt aaggctgtaa ataagaaata catacatggc
     7501 aataacataa tatacatgct atcatgacgc tgtaccccta gtttatgtaa aaacattccc
     7561 tcacataaca taagtaagaa tgatataaga aatccaatca agctattttt agttgacata
     7621 taattagatt tattagatat tattccacca agtatgaaaa atacaggtgc aaaaaatata
     7681 ccatttcgtg tataatctga tacttcaaaa atatacccat ataaattctt taatagtggt
     7741 attttttcta aaaaaccata ataactatca ccaaacatac caattacata aataacaata
     7801 gtgattacta atgagtactt taaacctagt ttttttacaa gaaagtatgc aatacttgca
     7861 ccaataattg atgcaggtag ataccataaa tgatataaag tcccatcaaa tacaatgtcc
     7921 ttaataatat tgggcactaa attattcatg gaaaaatatc cagtgtaaat atttaaggga
     7981 atataaatta caatagctat cccgtaaata aataatacat tttttagaaa agatttcagt
     8041 ttattacaat tataattata tgttgaaatt agaaaaaagc ctgatagcat aaaaaagaat
     8101 ggcacagcaa tacgagcaat tatactagtt aacacaaagt cagccatttt actatatgat
     8161 aataatggag atgtatgaat tgcaattata agtaatgcag ctataatcct aaaataatca
     8221 agccctgcat aagatttatt cctaatcatt cttgttcctc catttcttag tcctttctat
     8281 ttctccaaaa tgtcacaata aaaccatcct tattgttacc cgatatttga tatacacatt
     8341 gttcaggtat agagattctc acttcttctt tagtatgaat aggcacataa aagatttcta
     8401 taattttttc tttatcttct atttttaaac aatttgtata cgaaaatctc ttaatatatt
     8461 ctatatattc ttcaagggcg agattcattt tattgataat ctcagaatgt ggatatccga
     8521 cataacgaaa atgccatggt tcttgagata ttcctgttat ttcttctttt ttacactgat
     8581 aacgctctat aaaaccataa tgtagtgctg ccttacggaa ctcattacaa ataccattat
     8641 aaggaaattc agggcagata aaatctataa cctctttctt caaccctaaa tcaatagcaa
     8701 gccctgtctg atgttcactg tgatttggaa gtgctacaaa ctttttagta aagtcttttc
     8761 cattgtcacg cagagagctt gaatatatat cttgttgttc ctctgcagta cgatatccac
     8821 ttacaggtac tatttcattt attccacata tactttttaa aatattttgt aaaatatttg
     8881 tagcttcacg tttcataaga atacttggaa accttgtatc tacaggaatt aaatctatat
     8941 cttcagatgt tttaactggt aaaaaagcat ttactagaat tagattccca tcataaactt
     9001 tatctttagt caataagata gtatttgtca ttttatcttc tttattttta tctaataaaa
     9061 tattattcat cattttatgt acacctcaat tatcttatcc aatatttctt caaaagacag
     9121 tccaatacct ttcatcatat tcggataacg actatgagaa gtaaagccag gaattgtatt
     9181 gacttcatta aaaataattt caccagatgg agttaaaaac atatctactc tagcaaatcc
     9241 agaacatcca agagttctat aaataatttt tgctgtctct tgtattcttt tctcacaatc
     9301 caaatcaatt ctagctggca tatgaatttt tgaacttttc aatgtatatt tttctgtata
     9361 atcaaaaaat ccttttgaca attctatttc atctactctt ccaacaagta agtcatcgtt
     9421 tcctaaaact gcacacccta cctcaaaacc atcaatattt tcctcaatta ttacttcatt
     9481 atcatgttca aaagccaagt ctactgctac ttgtaactct tcttttttat aaattttact
     9541 aataccaaat gatgaacctg cacgcacagg ttttataaat aatggataag ttaagtcttt
     9601 tgacagttca gccacattaa atttatctga gtattttagt aatattgatt ttggtacttt
     9661 aataccttct gaatgaacaa gtttatgtga tttgtctttg tccatacata atgctgaaga
     9721 aagagtacca catccaataa cctgtatacc tgccaattca aataacccct gcactgttcc
     9781 atcttctcca ttttttccat gcaatacagg aaatacaaaa tcaagtttag tgtatttaat
     9841 atcattttca catgattcaa tagcactatt tggaatatga ttctgagaaa cagcatcagg
     9901 aattaaaaat tcaataatac catttttgac acggttctgc gaaacaataa ctggaattat
     9961 atcagtatcc ctttcaaacc aagtgttatt ctggatatta ttataatctc caatataatg
    10021 ataccaatta ccctcccttg taattccaat tggtataata tcatattttt ccgtattcaa
    10081 attctcaagt actgcaaaag ctgattgtaa tgatacatca tattctgttg agtttccacc
    10141 aaatataact gcaatttttt tattattcat ctttgattct cctttgttga tttatttatt
    10201 tttatacaat ctacagaaac atgttaaaat actttttcta tttcaaatcc aatcaacctt
    10261 cacttacaat tgcatctgtt ccacataata tattttcaca tttttctatt gatataatta
    10321 ctgtatcaaa ataaaagcac tcttgttttg atacagtaat tatatcaata acaagagtgc
    10381 tctttattta tttatcctaa agaatttcct atttttttat acttaaattc ttacaaattt
    10441 cctacaatcg gaataataac cttaaaggta attatattgt tctcgctatg agctgttatt
    10501 gtcccgttat gaagctccac tatttcttta gctatagcaa gtcccaaccc agctctacca
    10561 ctgtttgaat tacgagatgt atccaacctg taaaattgtt caaaaatacg ctcaagtttt
    10621 tcctctggaa tcgtattgct atgatttata aatttaagct ttatataatc atcctcctgt
    10681 acagctgtta tttcaatagt agtatcttca aaactataat aaacagcatt tcgtaataag
    10741 ttatcaaata cacgttgcat tttattaaca tcacacctta tcatcatatt tggtgtaaca
    10801 ttcagtgtat attttaaatt cttactcatc aaagtaggtt taaactcata tgttagttgt
    10861 tcaagcatac gagttaagct tactgtactg tattcaagtg atatatttga taaattaaac
    10921 cttgtaattt caaagaattc attaattaaa tcttcaagac gctctgcctt atccaacgaa
    10981 atagataaat atttttttcg cagttcttca gaaatctgac tttcatctcg aagtagtgtc
    11041 agatacccta taactgatgt aagtggtgtt tttaaatcat gtgcaagata aacaataagt
    11101 tcattcttac gttgttctgc catttgtgtc tcaaatttac gttgttcaag tatatgttta
    11161 attgagttta ttttcttttc tattgccaat aactctggag ataaagccac ttctccaaca
    11221 tcttctttga taagagagtc aataccttga tttatttcac taaaatattt tgtaaaccaa
    11281 tttaaataaa cacggaatat tataaaaaac acaatcacca tagctagtat catgaagagc
    11341 tccatgctat tacggaaaat ctgctgatat aaattcaatg cagctttata ttccatacga
    11401 aaagcatttt caaagaatga tactatccag atagaaaact taccatacaa tataaaagaa
    11461 tataaaatcc atatgaaaat aatggctata aatgccatta aagctgttct cataaaaatt
    11521 ttatttctca cttgagtaaa gtcaggattg actttaatat ttttactttt caattgtata
    11581 acctactccc catacagttt ttataaattt cgggttttta gaaggttcat gaattttttc
    11641 acgtaatcgc cctatatgag ccataactgt gttattatta tcaagatatt tttctcccca
    11701 tacagcttca aacaattctt cagaaggaac cacttttcct tgatgttcac ataaatacca
    11761 aagaatagaa aattcaatcg gtgttaatgc tacttctttc ccaaataaac tacatttatg
    11821 tgtttcttta ttgataatca atcctttaat atcatactca ttaacaatta tcgactgttg
    11881 ttcataagaa ttattatatc gcatatatct tcttaattgt gcctttactc ttgcaacaac
    11941 ttccaaaggg ttaaatggct ttgttatata atcatcagca cctatggtta aacccattat
    12001 cttgtctata tcttcaattt tagctgttaa cataatgata ggataatagt atttttctcg
    12061 aattttctgg caaatatgaa atccatcaat atcaggaagc ataatatcta aaatagccat
    12121 atccaaatct ttagattcaa tacattctag agcttcctta cctgtataaa acttgtatac
    12181 tgtaaaacct tcatttttta aatatacttc aattaaatct gctatttctt tttcatcatc
    12241 cacaatcaaa atattttcgt tcatagatta tttttcactc tccttattat cgaggtcatc
    12301 atttataata ttggtaaatt tacatatcaa ataattacaa gcatatttgt aactaatttt
    12361 tataagaaaa gaactactac atcagaatgt gttgatgcta ctattggaac acaaaaactg
    12421 agattaattg ttattataaa aactccccta acacgtcctt attatcgcat aacaggtatt
    12481 aactttcaat aaaaatatct atcagtcaaa cacaattcaa tttattatac cagagacaat
    12541 ccatataaaa aacaattaaa cctatgtatg taaccacaga cattactaaa atttccatgt
    12601 tattttatat aaaaattcaa aaaaggagtt gaatttaaaa tttgattaga aaaaaatcaa
    12661 ttttgagaca actccttttt attttaatgt atttaaatta tagtatatca gccttcataa
    12721 tatcctcaaa atcacctttt tcagatatta atcttatcaa tttaacagta cagcttaaat
    12781 catttataag tatagctcca actaatgaac catctttcat aaataatttt ttatatattc
    12841 tttgaccttc atcttttgat gaaatttctt tatcaaaatt ttgtatatta ccaatactaa
    12901 atacttttac attcattgca tctaatgaaa ctggatatac ttcactttca aatgtcaaat
    12961 tatctccact tgcatttgct cctgctattt ttcccatttc tactgaagat ggccatattg
    13021 ctaaactaat atttcccact tgtgcaacat ctccacaagc atatatatct ttaacatttg
    13081 tctgtaatgt cttatctaca acaatacctc tatcaatttt tacagatgta tcctgaacca
    13141 tttgagtgtt tgctcttaca ccaattgaaa atactaccat atcacagtca acactatctc
    13201 catctttaaa tacaactttt tgagcttttc catcaccttc aataccatca actgctacac
    13261 ccaatcttac atctattcct gtatctctta cacattgttc taatatttta ctaccctctt
    13321 tatcaagttg ttttgatagt atgctatcca tggcttcaac tactactact tctaatcctt
    13381 ttagtctaaa ttcccatgca gcttcaagtc ccaataaacc gccaccaatc actacaactt
    13441 tcttagattt ttctagtgct tcttttacac tatataaatc ttttatattt ctaagagtaa
    13501 acacattttc taaatcatgg cccttaatcg gtggtataaa gtttctactt cctgtagcta
    13561 ttacaagctt atcaaattta atagctccat cattcactat aatagtttta ttcacttcat
    13621 ctaatttatc tacattcgtt cctaatatta ctactatatt atttttatca taccaatctt
    13681 tatcttccat gtagaaatct gaaccaagtt cttcatttat accatcagaa agtgcaggtc
    13741 tatagtaagt caactcatct tcattagaaa tcatagttat cttacaagtt ttatttcttt
    13801 ttcttatagc atctgctgca taaaaaccag ctgctccatt tcctactatt acgaaatatt
    13861 catcagtatc ttttctaaaa gtaacttcat cttcttcaac ttctataaat tggtcttcct
    13921 tagctccaca tacagggcat acactaggag cactatcacc ttcaaaaact tcattacaaa
    13981 ttacacattt aaatttctta gttttctttt tacttccaaa agtagaattt aacttatcta
    14041 ggaatttacc tgtataagaa tgtaatttat caatctcttc atttgatggt ttaaagttaa
    14101 cagcaaaagg ttttactgct gtcattctaa gctgacttat tctctccata gcattttcta
    14161 taccttctcc actccatcca tatgaaccaa atactgaagc aacttttcca ccatgaagaa
    14221 ctgggtttaa cgatattaat atatcccata taggctttaa cgcatctcct aatattgttg
    14281 gtgttccaaa taatacacct tgtgatactg ctatttttgc caaaatatct tcttgcttat
    14341 gctctattac atcaaataaa tttactttat agttgctatt tttttcaata tatgctttta
    14401 tagcttctgc cataatcttt gtatatccat gtgcagaaac ataacaaatt gtaacttctt
    14461 tttctaactt tatttgttcc tttacactcc agttataata aaggtctata atttttctag
    14521 gattttcagt taatactgga ccatgacctg gacatacagt atctatatct aaatctttta
    14581 tcttttctat agcagttacc atagatggtt taaatggacc cattatacaa tcataatagt
    14641 atcttaaagc atccaagtaa tccttttctt cattttcatc aagtgtattt actatcttat
    14701 cattactata gtgactacca aatgaatcac atgttacaag agttttatcc tcttttatat
    14761 atgtgtatat agtatctggc caatgaagca taggtacaga gaaaaattct aaagttttgt
    14821 ccccaattga taatgtatct ccatctgtaa ctgcaacata ttcaaagtct ttgtttacga
    14881 tttccttaag atatttaatt gctgtttctg aagctactac ttttgcattt ttagctaaat
    14941 ccaaaagctt ttcaacgctt cctgcatggt ctggttcagt atgacttaca acaatatagt
    15001 ctattttttc aaaatctata tttaaatcat ttaatctttc aatataattg tcaaaatgct
    15061 tatctttaac tgtctcaaac aaaactgttt tttctgttcc ttttaataca tatgaattat
    15121 aagtagttcc ataaggtgta tacataatta tgtcaaatac tcttaaatca gggtcaagtg
    15181 aacctaccca atataaattt tcttttattt ttagtgattt caatgacata ccgtactcct
    15241 cctaaaatag ttatttagtc tctttatacc ctgcttgtta tattttaaac aaaatttatt
    15301 caaaaaagaa attattttaa agatagttta aatcttctaa tcttcttttt ctaccttcaa
    15361 tacagttccc tttttagaat ctacttttat gtcatattca tttttatctt tatctattct
    15421 tattttataa aaagtaacat ctaaatcttt gtctaaactc cattctttaa ccattccatt
    15481 ttgttctttt aatgcaattt ccatagcttg ttgaggtgta attatgtcat ttaaatctaa
    15541 tttttctttt ctagcaactc catttaaatc ttctgaatct aacttttctg ttttatcttc
    15601 taatacatct tttgtattag catcaatttt catcttatat tcattattat catctacacc
    15661 ttccacagta taaacaaatt tatttaaatc tctctcaaga cttaaatcta ctatatcagc
    15721 atccttgtat ttatctttaa atatgtttat tgcatctgtg tatggcactt taaaatcttg
    15781 ttccattaaa ttttcattat ttttttcatc ttttaaagca ttatttgaag catttgcctc
    15841 cttttttgta ttatcttgtg cttgtcctga atccttagtt gggctcccac tacatgcagt
    15901 taataaacct gctataagta tacttaccac taataaatac gtttttttac acataaaaat
    15961 cctccttaaa tatattaatt tattctatat tatgccctct aaacccttta tttacccttt
    16021 tatattaaaa attcctccta ttttatcaat tttaaaactt tatatttact aaaattaaag
    16081 ttacataaaa attatactgt ctcaaaatag aaacaagcat ctttttcatt gttgaaataa
    16141 taaaaatata ttttatttcg tacaaaaaga gtgcctcatg aactaaaaag ttcattttga
    16201 gacactccca ttcttttata tttttactta caaaataaca gtacttatta attttttaca
    16261 aaccttataa gtatatctat attatattct taaatttaaa cactatatta aaaaattaaa
    16321 ttttgtttaa ttcttttact gttaaatcca ttttaggata tggaatcgta attccttctt
    16381 cgtcaaacct aactttaacc tcttctagta aatcaagata aattgtccaa taatcttctg
    16441 tcttgcacca aactctagtt gcaaatttaa cagcattatc tccatgttct actactccta
    16501 taaatggttc tggctcattt attattaatt tatgattatt aacaatatct ttaagtactc
    16561 ttctaacatg tagtatatca tcttcatacc ctacactaaa agtcaaatca actcgtctag
    16621 tattctttgc tgaatagttt attagactat catttgatac agaaccatta ggtataagta
    16681 tctgtttatt atctggagta accaattgtg tataaaaaag tcctatttgt tcaaccgttc
    16741 ctccatggcc tgctgcttct acgtaatctc ctactttaaa aggtctaatc aatagtataa
    16801 taaatcctcc agcaaaattt gatagactac cttgtaaagc caaacctatt gctacccctg
    16861 ctgatgctat cacagcagca aaactggaca aactcattcc ccaatattcc agaacaatga
    16921 ttataacggc aactttaagt acactcaaaa gaagagactt caaaaacctt cttaaagtta
    16981 aatcaatatc tcttttacta agtactaaca taacatggtt tacaaatttt tttattattt
    17041 taaatccaat agaaagtata agtataccta ttataagttt tactccgctt gtagtagccc
    17101 attctagcag actttccatt atacctccca tatccatctt attaatgtct aaattattta
    17161 aatctaactt gcttatatca tctattttcg tcgtatccat aaaaaactcc ttaccttata
    17221 ttttagaacc aatattatag tttaaagttc ttattttcta tttaatataa tacaatacca
    17281 tttgtacata gtaaacaaga tttatattcc aattatacat cttaggaaaa tgcaaactat
    17341 ttgttatata tatgcaaaat ataatactaa attaaactat taataaaaca ctatatagcc
    17401 ttacaatcat cattttttac ccctatatga gatgatacta tagttataat acttagtact
    17461 ataaatacta caaaaccttt tttatatcca tcaactatat tatatgaatt tacttgtaaa
    17521 gagatagcta taatcaaatt aataattcca cttcctataa attcaaaagt attaataaca
    17581 ctagttgcca gtccagaata aatttcttta tttttatact tggcatcatt aaaaacctgt
    17641 agatgtgaca taactataca tcccataata aagaatgata ttggtaaaac tataattggt
    17701 ggttttacct ggtatacaac tacaattaca atccatataa acaaatctat cattgctcca
    17761 aattttatta tattaaattt gctagacctt attttagcaa atacaaaatc cataattatt
    17821 gaaccaaata taaatccata tgtaaaaaat gatactataa acgcagaaaa actcttactc
    17881 acaccataaa cgtccattat gtatcttact ccccacaagc ttataaatgc acttgtaaga
    17941 ccaacaaaag agaacattat cattgaatta taccatgttg atttgttctt tataacagac
    18001 ttaataccat caacaatatt aaccttctct gacttttcat agggttcagt atctacattg
    18061 aatccatact cttttggtgt atctctaaca ataatatata taaaacatcc aattacaaca
    18121 ccaattatac cgattaataa aaatgaattt ctccaaccaa caagttcaga caaaaacaca
    18181 agtggaaaag tagcaagaac tccacctaaa tttcctacaa atgaaaattt agctgttgca
    18241 gaagcaaatt ctgacttatt aaaccatctc ccttgaattt ttaaaataca aagtaatatt
    18301 actgatgttc ctgcccctac aattacacga gaaatatatg ctaattctat actttgaatc
    18361 aaaccaaata aaattgaccc taaacttgcc attattattc caagactgct aatttttctt
    18421 gctccatatt tatcaatcaa aattcctgca ggtatttgca taaatgcata tgcatatagg
    18481 caaaaggatg ctatatttga aatttgtata gaattaaaac ctaactcatt tgctaaattg
    18541 tctgaaacaa cagcagttga catccttaaa aataatacta aaacaaatga aaatgtaatt
    18601 ataccccaaa cactccactt agcctttgta taacttttca tatcttctcc ctcaaatcca
    18661 tatattttta aattttcttg atataactta tataaagtgt atagttattc ttacaaattt
    18721 ttacacgcaa tatcattata caacatatgt atatatttaa ctagaaataa aatactattc
    18781 atattatata catgaattaa ctacataaat aataagagcc atcttttaac attactcaaa
    18841 agatggctcc atataaaaac atttattcta aatttaattt tttatctaat ataaattttg
    18901 tagcgaaaaa taacgctatt gcaactacaa gactaccaag tattgcaagc ataaaactag
    18961 gactctgata aagatgatat accatatttg tatcttcatt tggtaagatg cttccaactg
    19021 catctccaac aattgatatt actatattta tagctataaa agaagcaaat gccactatgt
    19081 ttctatgttt attgaatttt ggtaattgtc ccattgagat tgatgtatat aataacaaaa
    19141 taaatgatga atatgaaata aacattacta ctactaataa taaaatatct aaaaagtttt
    19201 cagaaatcca tttaaaacta gtgttaaaca aatctaaaat ttcaggtagt aaaattccag
    19261 aagtaccaaa aagcataaga actccaaaag tgaatactgc aactataaaa ctaagtacag
    19321 cgtatataag tgctgttata catttagata atataagtga actagtgcta acaggaagag
    19381 taaacattaa ataaccttca tcccccaaaa gattttttct aaacctttga attgtaacta
    19441 caatagttaa aactcctaat gccataaaaa gacttgttaa tactagtatt cctattccct
    19501 gtacctgaag tatatttgta ttcataaata ttccattaaa tacagctact atcaatatgg
    19561 ctatatataa cggtataaat attcttccac ttgcttttaa ctcatatttt aataatttac
    19621 ctaacattta aattcctccc taaataatgc atctatagat tttccttttt cttctcttat
    19681 tgcttcaacg tctccttgta acactatttc tccttttgat atgaatatta tctcatcaca
    19741 tatgttttct atttcactta ttaagtgggt agcaatcagt aaagtactat cttcagaata
    19801 attttttaat atagtcttaa gtatatatga ccttgcagct gggtcaacac cacctatagg
    19861 ttcatccaat atatatatgc tcgcatttct tgacattact aaaataagtt gaactttttc
    19921 tttagttcct ttagacattg tctttagctt ttcatttaca tcaatatcta agcttttaat
    19981 catctcattt gctcttctaa catcaaaatc actataaaaa tcatagaaaa acttaagtaa
    20041 atctgatacc ttcatccaat catttaaata agttctttct ggtaaatatg aaactatttt
    20101 tttagtatct atggatggtt tcattccatt tatcataatt tcaccattat caggctgtaa
    20161 taatccattc attagcttaa tcattgtact tttaccactt ccattaggcc ctaacagccc
    20221 gactattttt ccctttggta tatttaaatc aatatttttt aaaacattct tagttccata
    20281 actcttgttt acacctttaa attcaactat tccatcttga gaaacattgc ttatattttt
    20341 atccatttta taattctcct ttcagatttt ctgtaataag ttttagcatt tcatctctat
    20401 catatccaag cttttctaag gttactttta atgtatttat ctccacatta gctatttcct
    20461 ctttcatagc ttttattttt tccttatcat ctgttacaaa tcttccttta gttctctgac
    20521 tataaactaa accttgtctt tcaagttctg ttagagcctt ttgcattgta tttggattaa
    20581 cttccgcatc ttctgctagt gctctcactg tttctaattt agatcctatt ttaatttccc
    20641 ctgaaattat ttttaacttt aaatgttcta ctaattgtat atatataggt ttgttattat
    20701 caagttccca ttccatgttc atgcctcctt attatattat tgtattaatt tattaataca
    20761 ataatataat aatacaaagt atatgtcaat tactttttag taaaagtata aaaaatttca
    20821 caaaaaaaat gaacactaca ttagagtcca tttttaacac aatttattaa attctttata
    20881 ttacagatta attaccctct taccaagttg gatacaatta aatgttcaaa ttcttcaatt
    20941 ggcattggct ttccaaacaa atatccttgt gccatatcac actttacttc ttttaaaagt
    21001 ttaaactgtt cttctgtctc aactccttct gatatgactt tcatacccaa ctcttttgcc
    21061 atttctatta ctttagtaac aattattttg cttcttttac tattataagt ttccataata
    21121 aatttttggt caagttttaa gatatccata ggaatttctt ttaacatatt taatgatgag
    21181 taccctgaac caaaatcatc catagatatt aaaaatccta ttttttttaa attattcatt
    21241 atatcaatta aaatatcaaa attatcaaat atgatactct ccgttaattc aagttctata
    21301 tactttgcag gtatattgta tttatcaatt atatttttgt atgtttctat aaaattattg
    21361 caatataaat gtattcttga aacattaact gaaacagtaa ctggatttat tcctgcatca
    21421 atccattttc taaggtatat acatacttct tcaagcacat ataaatctaa atttataaca
    21481 aaaccgtttc tttcaaaaaa tgggataaat tcatttggag gtattagccc tttatcagga
    21541 cgtttccacc taacaagtgc ttctgaacct tgtatctctc ctgtattaag atctatctta
    21601 ggttgtaagt atataataaa ctctttattt tctaaacttg aaaacattgt attttccatc
    21661 tctttttctt ttatctcttg gtcatgaatc ttattatcat aaaatgcata tgaactttta
    21721 tgtcctccct ttgccatttt atgaggtata ttagccctat ctatgattga gttgatatct
    21781 ctatcttctg gaagtataaa atagattcca caacttaaaa ctaacttata cttttcttct
    21841 tgattattat taaacaagtc taattcctca aatatagttt ttaacctctt tgttatatct
    21901 tcttcttcta tatatttcat aattataata aaattatccg cagatattct tgcaaacatt
    21961 tcctcttcaa atatattact cttaataatt tttgttaaat tacgcaaaat ttcattccca
    22021 acttcatatc caaataaatc atttatatat ttaaatttat caatatctat atataataaa
    22081 gcatacttta cttgagtatt tttacatagt attttgttag catcaattac aaatttatct
    22141 atagtatttg ctcctgtaag attatctgta tatgctatct tttgcaattt aattttaaaa
    22201 tttaatttgt catatattat atagaatata agcatcatac atattatagc cataataagt
    22261 attatttgta ttgcatattt gctaataaac tgattattag acacaataga ctcaaagtta
    22321 tttatggagt tgtttttata gagtttgcat aaagaataga ctggtgttgt gtatgtaatt
    22381 tgtaaaatta tacaaataaa taaaaaaaac atcaattttt tttgtaaatt cctcataaaa
    22441 atttcctccc attaaaacac ctctatttta ttagcaaata agtacatctc aaaagtttcc
    22501 atttcttgta aaatggatag tttatatcta ctttaatttt gtaaaatttt ctgctactat
    22561 aattatagaa tgtagcatat tcattgtctt aactattgat ttatatattt catataggta
    22621 taatagaaaa taaattggat tattttggca tataattatt tattaaaagg tgattatttt
    22681 tgtttgaatt gtctgaaaaa ttttattttt atattataat tttaatattt tcttttc
//
LOCUS       DAMKPG010000062        22314 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMKPG010000062 DAMKPG010000000
VERSION     DAMKPG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.065"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            258..1319
                     /locus_tag="PC621_002437"
     CDS             258..1319
                     /locus_tag="PC621_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF4109584.1"
                     /translation="MRDFLKNYKSSIILLACTIIGVIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKFLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1332..1529
                     /locus_tag="PC621_002438"
     CDS             1332..1529
                     /locus_tag="PC621_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF4109585.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1693..2421)
                     /locus_tag="PC621_002439"
     CDS             complement(1693..2421)
                     /locus_tag="PC621_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF4109586.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            2562..3287
                     /locus_tag="PC621_002440"
     CDS             2562..3287
                     /locus_tag="PC621_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4109587.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3347..4939
                     /gene="hcp"
                     /locus_tag="PC621_002441"
                     /gene_synonym="priS"
     CDS             3347..4939
                     /gene="hcp"
                     /locus_tag="PC621_002441"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF4109588.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5303..6208
                     /locus_tag="PC621_002442"
     CDS             5303..6208
                     /locus_tag="PC621_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF4109589.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6332..7285
                     /gene="msrA"
                     /locus_tag="PC621_002443"
     CDS             6332..7285
                     /gene="msrA"
                     /locus_tag="PC621_002443"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF4109590.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            7438..8568
                     /locus_tag="PC621_002444"
     CDS             7438..8568
                     /locus_tag="PC621_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4109591.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8685..9653)
                     /locus_tag="PC621_002445"
     CDS             complement(8685..9653)
                     /locus_tag="PC621_002445"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF4109592.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            9988..10176
                     /locus_tag="PC621_002446"
     CDS             9988..10176
                     /locus_tag="PC621_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109593.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            10436..11635
                     /locus_tag="PC621_002447"
     CDS             10436..11635
                     /locus_tag="PC621_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF4109594.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11687..12190
                     /locus_tag="PC621_002448"
     CDS             11687..12190
                     /locus_tag="PC621_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4109595.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12297..12614
                     /locus_tag="PC621_002449"
     CDS             12297..12614
                     /locus_tag="PC621_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109596.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12772..13731
                     /locus_tag="PC621_002450"
     CDS             12772..13731
                     /locus_tag="PC621_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF4109597.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13846..14631
                     /locus_tag="PC621_002451"
     CDS             13846..14631
                     /locus_tag="PC621_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF4109598.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14923..16242
                     /locus_tag="PC621_002452"
     CDS             14923..16242
                     /locus_tag="PC621_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF4109599.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16317..16745)
                     /locus_tag="PC621_002453"
     CDS             complement(16317..16745)
                     /locus_tag="PC621_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109600.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16836..18212)
                     /gene="hydG"
                     /locus_tag="PC621_002454"
     CDS             complement(16836..18212)
                     /gene="hydG"
                     /locus_tag="PC621_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF4109601.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18213..19298)
                     /gene="hydE"
                     /locus_tag="PC621_002455"
     CDS             complement(18213..19298)
                     /gene="hydE"
                     /locus_tag="PC621_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF4109602.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19334..20542)
                     /gene="hydF"
                     /locus_tag="PC621_002456"
     CDS             complement(19334..20542)
                     /gene="hydF"
                     /locus_tag="PC621_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF4109603.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20557..20808)
                     /locus_tag="PC621_002457"
     CDS             complement(20557..20808)
                     /locus_tag="PC621_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF4109604.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(21189..22229)
                     /locus_tag="PC621_002458"
     CDS             complement(21189..22229)
                     /locus_tag="PC621_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF4109605.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         8357 a         2671 c         3555 g         7731 t
ORIGIN      
        1 ttgtttgttt tattaaaatt aatctagaaa aaaagtttta aaagcagaat tttattaata
       61 tttatatata gatatgaact attaataaaa tattaaagat acaaaaaaac tatagaaatt
      121 gacatatatt ccagtatttg ctaaagtagc tgttggggaa aattacaaaa attattaatg
      181 cgcattagat aatttacttt aattttttta ggatttaggg taatttataa tataaaacat
      241 tagaaaggaa aatataaatg agggactttt taaaaaatta taagtcatca attatacttt
      301 tagcatgtac tataataggg gtgatagtag gattagtact tggtccaaaa gcttcaatac
      361 taaaaccatt tggagattta ttcataaatg ctctattaat gatagtagta ccattagttt
      421 ttcttagtgt ttcttcaggt gttgctagta taaaaaatat ggaacgtttt ggaaaaataa
      481 tgaagagtac agctatagta tttatgggaa caacacttgt aattgcattt ttaggtatta
      541 catctgcatt attatttaat ccgtctaaag gattagatgt atcaagtgta aaaagtttaa
      601 tggtggctca agatgcagaa actttagaat caaaaggaac gttattagac caagtagtat
      661 caagtattac agttaaaaat tttggagatt tattttccag tagcaattta ctacaattag
      721 ttatatttgc agttttagtt ggaacagcaa tagctatgat gggagatagg ggaaatacaa
      781 taagaaagtt tttatcagaa ggaacggaag caatattaaa agtagtaagt atagtcatgc
      841 tttatgctcc tatagggcta ggctgttata cagctacaat aattggggaa cttggaagca
      901 aaataattca aggatatgta agggtgtttg taatttattc actaacagca gccatgtatt
      961 tcttattatt ttacagtctg tatgcttttt tatctgcagg aaaacaaggg tttagacaat
     1021 tctggagaaa tgctataaaa ccaatagcaa cggcactagc tacttcttca agttcagcat
     1081 gtataccatc aaatttagaa gctagtaaaa atataggagt tcctgatgat atatcagaaa
     1141 cagttatacc tcttggaata aatatacata aagatgggtc agttttagca gtaataataa
     1201 aagtggcatt tttatcaggt attttaggca gtggaatggc aaatccagca acgataggtc
     1261 aattatttat aactgtattt gttgttggag tatttatggc tcctgtagca ggtggctgac
     1321 ttattggtga gatgctcgtg gttagcatct tcggttttcc agtggaggca attccattgt
     1381 taacagttat aactactata actgatattc ctaatactat attaaataca actggaaata
     1441 cagtatcatc aatgttagtt tcaagattag tagaaggaaa agattggctt atagataaaa
     1501 cagcaattac tacaaagaaa ataagctaat tgtacactta attaataaag ttaataggta
     1561 tcttaatttt aataaattag atatctatta actttttaag taaaaatata aatttaagat
     1621 tcaaatatga tatttataat ttgaattaac taatatagta tatattgaaa taaatatgtt
     1681 taaatatcta gtttattttt gaaagtaaaa gatttcaaat ttatttttat taaaagataa
     1741 caagccttca tctcgcatct tgccaagctc tcttgacata gaacttctgt caacacataa
     1801 aaaatcagcc atttcatttc ttgaaaaggg aattgtaaac tggtggctac ctgtttgttc
     1861 tatctgtttt gaaaaataca taagcaattt ctcgcgagtt gttttacgag aaagtaattc
     1921 tattttattg tgaagtaaaa tgtttttttc agctattaat tgtagcatat tttcaacaag
     1981 cctattatga aatacacatg cagatgaaca aataccagtt acacttttaa actttataaa
     2041 taaaacttcg caagaagatg ttgtggttac tgtaacatta cttttatgta gtctagtaca
     2101 tgcgaatacc tctccaaata aatctccagg caagagttca gctacaatat tcctattccc
     2161 ttcgatatct tcctttatta tatgagcact tcctttaagc ataattccaa tagaagtaac
     2221 ttcacttcca acagagaata ttatactatc ttttgggtaa gtcttatcta caacacctaa
     2281 acattccaac atttcttcaa gctcatcact tttaattcct tcaaatagag gaattttttt
     2341 aagtaggtct atatgctttt ctaaaaattt tttcaaataa tcacttcctt gttgcatatg
     2401 caaccgataa tttgttttca ttgtactata ctaagtttat aaaagcaagg ggatataaaa
     2461 atcctactac aagaaatttg aaataaagaa atataggtat taaaaataaa tatataagaa
     2521 atcaatactt taaattaaaa aaattttatg aggtgatata aatgatgaga aaaataatta
     2581 aaattgatga gggaaagtgt aatggttgtg gactatgtgt tgaagcttgt catgaagaag
     2641 ctataggaat ggtaaatgga aaagcaaaac ttttaagaga tgactattgt gatgggctgg
     2701 gagattgtct gccaacttgt ccaacaaatg cgatttcatt tgagtataga gaagcagcag
     2761 aatatgatga agctgcagta aaagcaaata tggaagcaaa gaaagcacaa aagaaaactt
     2821 tagcatgtgg atgtccaggt tctcagtcta agtcaataaa tagagaggtt tcaaatagta
     2881 catctataag caatgatata gtagagataa aaggttctca acttaatcaa tggccagtac
     2941 aaataaaatt agtaccaact aatgcatcat atttaaagaa tgcaagtctt ttaatagcag
     3001 cagactgtac agcgtatgca tatggaaatt ttcataataa atttatgaaa aataaggtta
     3061 cattaatcgg atgccctaaa cttgatgaag ttgactatgc tgaaaaactg acagagattt
     3121 taaaagaaaa tgatattaaa aatatagtag taacacgtat ggaagttcct tgctgtggtg
     3181 gtatagtaaa tgctgttaag acagctctac aaaatagtgg aaaaatgata ccatggcaaa
     3241 tagttactat atctgtagat ggaaaaattg tagatggaga aatttaatta agtgaatcat
     3301 agaaaaatat aataaatttt aataaataaa aagggaggaa taaaaaatgg aaaaaatgtt
     3361 ttgttttcaa tgtgaacaaa cagcaggagg taaaggttgt actataaatg gtgtttgtgg
     3421 taaaaagggg ggtacagcta acttgcaaga cgagcttaca ggagtgttga ttggtctttc
     3481 aagagcaact attggaaata agaatagacc aacaagtgaa acagataaaa ttatgataga
     3541 ggggctattc actactataa cgaatgtaag ttttgatgat gaagctataa aaagacaaat
     3601 tgaaaaaact gaggtagaaa aaagtaaact tgtacctaga tgttcagatt gttcaacaac
     3661 ttgtaataga aatgataact atgatatgaa ggatttatgg aatgataatg aggatatacg
     3721 ttcattaaaa tcattaatct tatttggatt aagaggcatg gctgcctatg catatcatgc
     3781 aatggtactt ggttatacag acaaagacgt aaatgaattt ttctatgaag gattaatagc
     3841 agttggagga gacttgtcta ttgacgaact tttaggtcta gttatgaaaa ctggagaaat
     3901 aaacttaaaa tgtatggaat tgcttgatag agcaaatact gaaacttatg gaacaccaga
     3961 accgactcag gtatctatga aaatcgagaa agggccattt attgtagtta caggacatga
     4021 cctttatgat ttacacaaat tattagaaca aacgaaagat aagggtataa atatatatac
     4081 tcatggtgag ttattaccag cacatgcata tccaaaacta aaagaatata aacatctaaa
     4141 aggtaatttt ggtacagcat ggcaaaacca acaaaaagaa tttgataata ttcctgctcc
     4201 aatattattt acaacaaact gtttaatgcc tgttaaagat agttatagag atagagtatt
     4261 tactacagaa gtagttgctt atccaggtat ggttcatatt gatgaagata aagatttttc
     4321 tagtgtaata gaaaaagctc ttgaacttgg aggatataga gaagatgttc atatgactgg
     4381 tattaatgga ggagatacat taactactgg ttttgcaaga ggtacagttt catctgtaca
     4441 agataaagta gtagatgctg taaagagtgg agctataaga cactttttat tggttggagg
     4501 ttgtgatggt gcaaaacttg gaagaaatta ttacactgaa tttgtaaaac aagctcctaa
     4561 agatactgtt atattgacac ttgcatgtgg aaaatataga tttaatgatt tagaccttgg
     4621 ggatataggt ggcttacctc gtttaatgga tatgggacaa tgtaatgatg cttatagtgc
     4681 aattaaagtg gcagtttcat tggcaaatgc ttttgaatgt ggagtaaatg atttgcctct
     4741 tagcatggta ctgtcatggt atgagcagaa agctgtttgt atacttctta cactacttca
     4801 tttaggtatc aaaaatatct atatagggcc ttctttgcca gcattcttat ctaagaatgt
     4861 attagatata ttggttgata aatttgcatt gacacctata tctactcctg aagaagatat
     4921 gaagaagatt ttaggataaa taaatatatc tatacttaaa gatatttata gttaaaatat
     4981 ttttaagtat gtcacaatta agataagggg ttaaacttaa aatttgatta aaaaatcaat
     5041 tttgagacga ccctttattt attttttgta ataattgaaa aataaacctg ctttcagtat
     5101 attcttttaa gaatttttgt attaaattta taatacttag atatataata tcttatatat
     5161 tttattttaa gtactaaagg tttgaatgta acttaaatat taaaagagga acattgtaat
     5221 atgtaacttg cataaaatat atatactata gtgaaattat tttgattaaa atataaggtg
     5281 ttttatgtgg gggtatatgt atatgaaaag ttctaatttt aatatattgg catttgatgg
     5341 tggtggatta aaaggggcat tgagtatatc catacttgag aggatagtaa aagagtatcc
     5401 aaatctatta aacaatataa atatgtttgg agggacatct acagggagtc taattgcact
     5461 aggacttgcg tatggtgtaa gccctaaaga gataaaagaa ctttactcaa ttgaaaattc
     5521 taagtatatt tttaataaaa gttatgcaga aatactcaga ccaaagtatg aaaacaaaaa
     5581 cttaaaagaa gttttattaa gtatatttcc agaggaacta gaattaaaag atttaaataa
     5641 attagtaatg ataccaagtt tttatatagg aaatgaggaa aatgcatgga aaccagtgtt
     5701 ttataacaat atgccaaatt cttttacaaa gactagcaaa gttgtagatg tagcaatggc
     5761 aagtagtgct gctccagtat tttttccaac atataatcgt catgttgatg gaggaataat
     5821 agcaactgac ccaagtttag cgtgtataat tcatgccatt gacagtggat ttaaacttaa
     5881 gaatacaaga cttttttcaa tagggactgg atatgtatat aatagtataa aggcagatac
     5941 tactgaatgg ggagctattg actggataat taataaagaa cccgatttac caattatatc
     6001 tattacctta gaaggaaatt ctcaaatgag tcagttattt tctcaaaaac ttcttggaga
     6061 taattactat agattaaatc ctaagatgga aaaagatgta gctatggatg attgtgatgc
     6121 attagaatat ctaatgtctt taggagaaaa ttgtgatata aaagatgctt ttggatggat
     6181 aaagaaaaag agcttttata gaaattagat tcaatttgca attgtatttt tgcacatgat
     6241 ttcattgagt aggtaaaaat aataaaatgc agattattaa gttacttctt agggaataaa
     6301 taaagtataa tttatttaag gagtgatgaa aatgataaaa cttgctactt ttgcaggtgg
     6361 atgtttttgg tgtatggtaa aacctttcga taagtatgat ggagttatta aagtagtttc
     6421 tggatataca ggtggtcatg ttgaaaaccc tacatatcaa gaggtatgtt caggaactac
     6481 tggtcattta gaagcaattc aaataacata tgatgatgat ataataagtt atgatgaact
     6541 tctaaatatc ttctggaaac agattaatcc tacagatgat ggtggacaat ttggtgatag
     6601 gggaactcaa tataagactg ctatatttta tcatgatgaa gaacaaaaaa atatagcttt
     6661 agagtcaaga gaacatctgg aaaatagtaa aatatttgat agtaaaatag tgactgatat
     6721 aattccagca actaaattct acgaagcaga agaaaatcat caggattatt ataaaaaaag
     6781 accattaaac tacaatatgt attataaagg gtctggaaga tataattttg taaaaagtca
     6841 ttgggatgga aacaatataa atagggaaga gttaaaaaag actttaactc ctatacaatt
     6901 tgaagtcact caaaatgata tgacagaagt accttttgaa aatgaatatt atgataataa
     6961 caaagaggga atttatgttg atattgtaag tggagaagtg ttattttctt caaaagataa
     7021 atttgattct ggatgtgggt ggccaagttt ttcaaagcca gttatagata aatctatcat
     7081 tgagagaagt gatttctcac aaggtatgta cagaattgaa gtaagaagtg ctaaaggaaa
     7141 ttctcattta ggtcatgtat ttgatgatgg tccaaaagaa cttggaggtt taagatattg
     7201 tataaattct gcttctctta aatttatacc aagagaaaat atggaagttt taggatatgg
     7261 agaatattta aaactttttg agtaattagg aatatttatt ataaacttat ctaaaaggtg
     7321 aaaatataaa aaaatatttt caccttttgt ttttttgtca ataatatgat aaattattaa
     7381 ttgtaatctt atttatacca atagattaag aatttaattt tgaaagagag aaaacaaatg
     7441 aatttaaaag actttaatgg aaagagatta aaatcagcta gaatttttag agcaaagacg
     7501 atagaacagt tgtctaaaga aactaaaata aataaaaaag atttaaaagc ttttgaggaa
     7561 aataagtatg taccaaatat agaaaataca ttaaaattat ctaatatatt aaattttcca
     7621 aaagagtatt tttataaaaa tgaaaatata aatatcgttg tagaggatag ccattttaac
     7681 cctcaatcta aactacctag agtagaagag atttcttata gagaaaaaat aattataata
     7741 cataaaatat attcatttat ggagaattat attaagtttc caaaagaaaa cttgccaaat
     7801 agaaaagtaa tgtcagatat taatataagt gatatagaat ctcttgcata taagactaga
     7861 gagttttggg atttaaatag tactccaata gtaaatatgg taagtttgtt agaatctaaa
     7921 gggattataa tatcaggtat gaatgtagat agaaaagggg caactatctt tactcaaaaa
     7981 cagagaatta gtaaagaatc taaatatcta atatctctgg gtaatgacaa aaagtcagca
     8041 tcaattagaa attatacttt ggcatgtgag cttggatata taatatcaag tgaattaaga
     8101 ataccaagta agcaattttc agaagatgag tatgcctgtg catttttatt gccaaaagag
     8161 agttttttaa aagatttgac tcatccagaa gatttagatt attatgtaga actaaagaaa
     8221 aaatggattg tacctatatc tgctatgatt tttagagcat ataatctaga aaagataaat
     8281 tacaaaaaat acaattatct tatgaatgaa atggataaaa aaggctggtt gatagaagaa
     8341 ccactagaca aaatgaaagg ttcaagccca acgtatttaa aaagagcagt tgaattattg
     8401 atagaaaata aaataatgag tgtaaatagt atagtatcaa gtttagagga atttggaata
     8461 aatctttatc cagaagactt agaattattg atgggattga aaaaaggatt gttatctcaa
     8521 gaggtaaata aaaaaagtaa agttataaaa tttgatggta aaaaataggt ttatatttta
     8581 gtattaaaat taaaagcagg tataaatgat ttaaacatta acttagttaa aagtcttttc
     8641 aataagtcat ttacctgctt ttagtgtaag aaagaatatt gaatctaaag tgtttttgaa
     8701 agtgcaatac attctcttaa aacagagcaa gatttattaa attcgttttg ctcatattca
     8761 gtcatattga tttcaataat ttcttcaata ccatcttttc ctataacaca tggaacacct
     8821 gcaaatacat ttctttcgtt atattgacct tctagtaatg tagaaactgg aagaactttt
     8881 ttctcattgt gtagtatagc ttttacaatt tctactaaag cagtacctat accaaattct
     8941 gtacaatctt taccatttat tatacttatc cctgtttttt tatttttttc aactattgat
     9001 ggtaagtcta aattgctata agtatttggg ttgtctttta tcatgtccaa gattggttta
     9061 ccaccaattg aaacatgaga ccaaggaacc atttgagaat ctccatgttc acccatagaa
     9121 taagcttgaa ttgattgttg agcgattcca gtttcttcag ataaaatcct tctaagtttt
     9181 gttgaatcta aagctgttcc agttccaatg actttatttt taggaaagcc agatttttcc
     9241 caaatataat ttgtaactac atcaacagga ttagatatga ctacaaatat accatcaaag
     9301 ccagaatcaa ctatgggttt tactatagat tttatcattc caatagttgg tcttagtaaa
     9361 tctaatctag ttgtgttttg tctacgtaaa ggcccttctg aagagtctgc aacacttatc
     9421 actactatgt cagaatcttt acagtcagaa aaagttcctt tttctatatg tactttatgt
     9481 ggtagatatg aaactgcatc agctaaatct aaagcttgag cttttgattt agattcatct
     9541 atatcaatca taaagagttc atcacaaact ccttgagtta ttaaactaaa tccacaatga
     9601 gaaccaacat gccctgaacc tacaatagat atttttcttg gctttattgc cattttaaat
     9661 cactccccct aaaataacga tacattgaaa attatagatg ttatcaaaag ctgtttttgg
     9721 atattttaat attttattat ttcagaaaat ttatttaata tgattttata ctaaaatttt
     9781 taaaaaagca atgttaatat actctctatt aatgaatgtt aaagtattat atgtatatta
     9841 ataattatta aaatatgtaa tgtcactgat agttagaata tttaagtgtt aaaagatata
     9901 tataaataat aatgatatca gttgataatg atattaaatt gaggtatact gtttctctaa
     9961 attaaaaaac aaaggagtaa atgtgttatg aattatcata caaaagaaga gttgatggaa
    10021 gtactgagag tagcatcttc aaggattatt aattgtgaaa aagtacagaa gaaattttct
    10081 gaggagacat ctcatcatac acgttttaag aatattatag aagcaatgta tatttcaaaa
    10141 tcattaataa tggatgaaat tagtaaaaga gattgaagct tattttgcaa tttaatatgt
    10201 tttaatatac attttattat ataagaaaat aagtaagcat acttgttata aaatattgaa
    10261 ataatataaa attataaatt tgttaacact aatatctata tgttaaaata tagtagaaga
    10321 tgttagtaaa taataggatg agttctattt taatacaatt aaattaagct tagcttgaaa
    10381 tttgtattta aaaagtaaag tagtctataa ttaagaaaat ggagatagaa ttataatgga
    10441 aaagttatat tatacaaatc agtatataag agattttaca gcagaaataa ttgaaataaa
    10501 agaagtagaa aataagtatc atgttttatt agataagaca gctttttttc ctggtggagg
    10561 aggtcagttt ggagacctgg gaaaaataga gaatgatgat gtaataaatg tgtatgaaga
    10621 tggaaaaaat atataccatg tattagataa aaaacctata aagatacata aggtaaagtg
    10681 tagtattgat tgggatagaa gagaagatgg aatgcatcaa cattttggac aacatgtatt
    10741 atctggatgt ttttttaaaa cttttaatgc aaatacgaca ggttttcatt taggaaatga
    10801 gtatagtaca gtagatatag aaggtaattt agaagagaac aaaattagag agatagagtt
    10861 gtttgccaat gaaattattc gccaaaatat acaagtagag acattacttc caacgaaaaa
    10921 agaactaaaa aagatttggt taagacgtga tttgccaaat actgatgaag agataagagt
    10981 aataaaaata ggtgatttag atacgaatgc atgttgtgga gtgcatccaa aatcaactgg
    11041 tgaattgaga atgataaaaa taaagaaatg ggaaaaacat aagtcaagta caagaataga
    11101 gtttctagca ggtaaaaggg cagtggatga agtattgaag agggatatat atttgactaa
    11161 aatatgtaga tatttaagct gtagtgagga agatgctata aatggtattg taaatctaaa
    11221 tagtaaagtg gaagaggctt taattaaaaa aagaaggctt gaagaagtag tatctacata
    11281 tcaggtcaaa gaaatgattg aaaaagcaaa taaggttggc aatatttcgg tcattaaaaa
    11341 aatttatgat gatgaagatt tgaaatatgt aaataagata gcaaataaaa ttgcagaata
    11401 tgaaaatagt attgcattat tagctgttaa atttaatgat aaaataaatt tagtttttgc
    11461 atgttctaaa aatttaaaag atattaatat gggagtttta ttaaaggatg caataagtct
    11521 tatagatggc aagggtggtg gaagtaaagt attagctcaa ggaggaggta agaataatgg
    11581 aaatttagag gctacacttg attatgcatt tatgaagata gaaaaaactc tataaatata
    11641 ttatataaaa aaccttagta gataaaatag atatggagga ataagtatgg agcaacaaaa
    11701 ggactacata gagttatatg aaaatgaaga actaatagag ataagagaag cacgtatgga
    11761 tgatttggat acaatagcaa aatttaatta taatttggct aaagaaactg aaggaaaaga
    11821 acttgatatg gatgttttaa ctaaaggtgt aaaagcattg ttattagatg agagaaaagg
    11881 aaaatatcat gtatatacag tatttgataa agttgttgca cagattatgt atacatatga
    11941 gtggagtgat tggagaaatg gaaatttcct ttggattcag agtgtatatg tagataagga
    12001 atatagaaga aaaggtatct ttaattattt atttaattat ataaaaaata tatgtgataa
    12061 agatgaaaat atagttggaa tgagattata tgtagaaaaa gaaaacataa atgcaaaagc
    12121 aacatatgag tctttaaata tgtatgagtg tgattataat atgtatgaat acgaagtaat
    12181 acattcgtaa tataaatttg taatactgtt atactgatgt tataaagtat tgattatatt
    12241 gacgattata tgaaataaac tggatattat aattagaatt tttagggggt ataaaaatgg
    12301 gcgttttaaa aggaatgttt tatgtatttt tatttttaga tttagtgtca atattctgtt
    12361 tctttttcaa taaaggaaaa atagcatcta ataaaattgt gtttaatgca ataggagtac
    12421 ttacatttgt actttgtttt atgcttttta gttattatcc taataataat cttattggaa
    12481 aatttattgc ttctctattt tttatatttg gagttgcagg agttatgttg aaagaaaaaa
    12541 attttttata tgctagactt cttttaactg tagttatagt tttttctaca ttaagattgt
    12601 tcgttattca ataaaatgta tttgtatgaa aatttattaa agtctacaca tttaagtgta
    12661 ggcttttttg attttttgtt tgaaaattaa ggaaatttta aggaaatttt aaaatcgtct
    12721 taaatattta ttgatatact taaaatatta atagcgaaag gagacttatc agtgatagaa
    12781 agtaaaaagt cagaaaaatt gttactgtca attatataca taatattttt ggtatatatt
    12841 ttaatgatta taagaataat tttatttaga gatgtaccaa tttacgctat tttcaaggga
    12901 acgtttagga atgtaaattt aattccattt tatacaatat atcaatttat agtagatagt
    12961 aatcttgact ttatgaaagc tactgtaaat attatagaaa atataggtat atttattcct
    13021 atgggaattt ttttacctat agtatgtaaa aatctaaata aaaaaactat aattatagta
    13081 attatacttg ttagcctagc atttgaatta acacaatata tttttgctct tggaagtagt
    13141 gatatagatg atgttatttt aaattcatta ggtggaataa ttggaattaa tatttacata
    13201 aacatgaata aattatttcc taatgatatt aaaagattta aggctataat agctactagt
    13261 ttagttttag gagttttagg attaggagtt atttctaaaa attatcataa cttactaact
    13321 tttaaattta gacctgatag aaaaatatca aaaatattaa ttgaagaaaa tagggaaata
    13381 attaaagata taaataaaga tacagtagat atagttggga catttgagag ctttaaaaaa
    13441 ggaataataa ctattaaagc aggttctaat aacaaagtaa agagaccaga gaatttaatt
    13501 gattctgatg gaaatataag aatttattta aatgaaaata caaaattagt atcttatata
    13561 attaccaaag aatcaaaaat ggatatagtt aaatatgagg aatttgatgt gaagaattta
    13621 gatttattaa ggagatatga tactattaat gtttggattg ataaagaaaa tcagccaaag
    13681 gataaacatg gtattatggc tagtaaatta ttaattggat tgtatgaata aaatattaaa
    13741 caagaataaa agaataacaa atataaaaaa cgaaaaaact atttctataa ttatttttac
    13801 gttgtgcata aaataagaac attagtgaag gagtagagat aaattatgga gataataaaa
    13861 tatatcttgt acttttttat atattcattt ttaggatgga cagtagaaag tataggatgt
    13921 tctatagcaa gcaaaagaat aatcaacaga ggttttttaa atggtccaat atgccctgtt
    13981 tatggttttg gagcagtaat tgtgatatca ctattaggaa gatttaataa tgtagtaata
    14041 gtatttctat taggaatgat tttaactacg atactagaat attttacagg atttatttta
    14101 gaaaccttat ttcatgctaa atggtgggat tattctgaca gaaaattcaa tataaagggt
    14161 agagtatgtt taaaaaatgc aatatacttt ggtgtaatgt ctgttttaat aataagattt
    14221 atacatccat ttataaaata ttttgtaagt ataattccat atagaatatt aatttcaatg
    14281 gctataatta caacactttg gactatactt gatttgattg ttactattat aacacttaaa
    14341 aaattagata taaagttaaa cttactagac gatataatta cagaccttaa tgatataaat
    14401 gtgaaattgg ataaatttga tagaggagaa atacaagctt tatttaaaac aataaataac
    14461 gacggattag aagttagaga aaaaataaac aaaataaata gtaagttgga ccgtatagag
    14521 tctaatatta ttttacaaaa aagagttata aaagcatttc cacatataaa acataaaaaa
    14581 caacaagagc aactagaaca ttttaaaaaa attattaatg agaaaaggta attatacaat
    14641 ttaatgaaga cgaatagaat aatggtattt ataagattat tttcagagga ctctataaaa
    14701 atagaataga gtcctttatt ttttgaaaaa taatgtttat ttgataaaaa taaaatatat
    14761 tgtttctaaa taacaaatat tttgaaaatt ataaaaacat atattgacaa tttgtgtcaa
    14821 aaatactaac attaagtata taaagaattt tatatgagaa tatctttata aatcgcaata
    14881 attttagaac ataaatttat ttaaagaggg gatgatttgc caatgagcga gataactagt
    14941 caaatgataa gtacagaaga aaaaaaatat gttgctaaaa ctcaaaagat accttattat
    15001 ccagtagcat ttaaatcagg tgatggtgca atgctatatg attatgaagg taatgaatat
    15061 gtagattttt tagcaagtgc aggctctgca aatgtaggtc atggtaataa ggaaatctca
    15121 caagcagtta aagaacaaat ggatgatata acacaatata cacttgcata tttccatagt
    15181 gatcctcctg taaaattagc tgagaagctt gtggaaatag cccctggaga taatgataaa
    15241 aaagtactat acagtgcaac tggttctgct tgtatagatg ctgctataaa attagcaaga
    15301 gggtatactg gaagaacaaa gataatatct atgtgtgaat cttatcatgg aagtacttat
    15361 ggagctatat ctatatctgc tttaagtaca aatatgagaa gaaaaatggg tccattgtta
    15421 ccagaagttt atcacttcca ttatcctgat aaaaatagaa cagctaaaga atgtttagat
    15481 gagatagaat atgcttttgc acactatcta ccagcagaag aggtagctgc aatatttata
    15541 gagccaattg caggagatgc aggaataata gtgcctccag tagagtgggt acaaggttta
    15601 agtaaaatat gtaaagaaaa tggaatatta cttgtaagtg atgaaataca acaaggtatg
    15661 ggaagaacag gtaagtggtt tggtatagaa aattttggtg ttgaagcaga ccttatagtt
    15721 cttggaaaat ctgttggagg tggattacct ttaggagcag ttgttggaag aacagaaata
    15781 atgcaaagtc ttgatgcacc agcacattta tttactttag ctggaaatac aacagtgtgt
    15841 gtcgctgctt taaaatctat agagataatt gaaaaagaaa acttacttca aaagagtata
    15901 gaaatgggag attatattaa agcaggattt gaaaaactaa aagaaaaata cgatataatt
    15961 ggagaaataa gaggaatagg gttatctata ggagttgata tagttaaagg aaaaggttct
    16021 aatgaaaaac atccagatgc tacagctaag atatgttata gatgtataca aactggatta
    16081 ataatgatat tcttaggaca atctacttta agagtacaac cacctctagt aataactaag
    16141 gagcaagtag ataaagcaat gagcatcata gattctgcaa tagatgatta tttaaatggt
    16201 agaattggtg atgaagtata tgaagtaact caaggttggt aaaataaata ttaaatgaaa
    16261 aaaagagtta tctcaatttt agaaagtaat ttctgaaatg agataactct tttaagttac
    16321 ccacaaataa gaaaattgtt tgattcatta aagactatat ttcggatgtc gtttttgtga
    16381 gtatcagaga cagtattacg agaataatta cctttattcg tactatctgt taatttagtt
    16441 ataattaaga tagctaaaag aataagaatt actgtgtata aagcaataac atatggtttt
    16501 cttttatatt tgtatttgtt attgaaaaaa gaatggccat aattattttt atttacttta
    16561 gatgtatttg tattcttttt cttgttacct ttctttttat catccttttt tctattttta
    16621 gggtaaggaa tgactttgtg tgtattatca agtttgatat ttttattttt ttgaatattg
    16681 ttttccaggc tagccttttt ttctccaaat tgaatagttt tattggtatt gttgttatct
    16741 ttcaatgtta atctccctcc cttaaacttt ccctttagat aatattacta ctaacctatg
    16801 gaatatacaa taaataatat ataagtaata aaattttata ggtataaatc tctttctcca
    16861 cgctctaagg cagtcatatt gttagatact atgtttctta tatcttctct ttctatctta
    16921 cctatttctt ctgcgataag ttttttaccc ataattttaa gttcttcatc tccataatcg
    16981 attaaaaatt catttaatgt agtaagagca tttggcgtac atacattatg gatttgtcga
    17041 cttttagcta agctcatgaa tctatcgcca gttctacctt tacgataaca agctgtacaa
    17101 taactaggta tatatccatc agtaatcagt tctttaagca cctcaacagg gcttctatgg
    17161 tctccaactt cgaattgttc tgtattgtca ccatcttcat atgctttata tcctccaaca
    17221 cctgttagag aacctgcact aatttgtgat acaccatatt ttagtaactc atttctcatt
    17281 tcagcagttt ctctagtgga cattatgatt ccagtaaaag gaacagccaa tctagttata
    17341 gctactattt ttttgaacat atcgtcagaa actaagtgag gaaaatcttc taagctcata
    17401 ccttcagctt ttttcaatct agggaaagat atagtatgga aacctacacc aaatttttcc
    17461 tctaagtgtt cgttgtgcat cataagtcct agaacttcaa atttagggtc agataaacca
    17521 aataaaactc ctgcaccaac atcatcaaca ccagcttcca tagctctatc aaaagctgtt
    17581 aaatggtaat aatagtcatt ttttatagat tgaccatgca tttttataaa tgttggtttg
    17641 tgataagttt cttggaataa tatataagtt cctattcctt tttcttttag taatttatac
    17701 tcatctacac ttgtagctgc tatgttaacg tttacccttc ttatatttcc gttttcatta
    17761 tatgttgaat aaattgcatc taagcaatct aagatgtaat ttatatcaca atttttaggg
    17821 tcttcaccag cttctagagc aagtctttta tgacccattt tttctaatat tttaacctct
    17881 tcctttatct cttccatggt aagttttttt cttttaaatt tgttgcattt actaaaaccg
    17941 caataaacac attcatttac acagtagttt gatacataaa gaggggcaaa taaaaccact
    18001 ctattaccat atatttcatt ttttatttgc ccagctatac taaataatct atctagttgt
    18061 tttttatctt ctacttcaag aagtgtagct atatctttat aactaagttt ttcccttcta
    18121 tcagccttat caagtacctt ttcaatatca tcagaagttg aattttttgc atcttctaat
    18181 aaagaattaa taagttcatg gtttatgaac atttacaaac gcctccattc aatattatca
    18241 ccgcgagaat agtcaacttc aagtcctaat gacttgatgt tttcttctaa actttggtga
    18301 tattcacaag cttcttgacc agtaaaggct ttgttatcgt aaagagaata ttgttttcta
    18361 aattcttgag gagaaaggtt tggcataatt acattgcaac cagctaaaag acctgcattt
    18421 ctaccactag ggtttataga tgccaaggct gttgtagcgg gtaataaaac ttttggaagt
    18481 agaagtcttg taattgaaag cataagtaat gtcttctcta aatctccatg tttataatcc
    18541 ttaaaaattg tatcatggtg tggaataaaa ggtcctattc caaccatatg aggatttaac
    18601 tcttttaggt ataataaatc acgaacaagg tctttgttgt tctgaaatgg tgattcaacc
    18661 ataaatccag cacctacctg ataaccaatt tctttaaggt tttttaggca ttcttttcta
    18721 gttctaagct caatgtttgg cggatgtaat tttttataat gactgtctgt agcagtttca
    18781 tgtcttaaaa gatatctatc tgcaccacat aaaaagtatt ttttatacga atcataactc
    18841 ttttctccta gtgacagagt tatggcacaa tcctcatatt tattttttat acttgaaaca
    18901 atttcacata ttttatcatc agtaaaatat gcatcttcac caccttgcaa aacgaatgtt
    18961 ctgtatccta ttttataacc tatatcacaa cattcaagta tttcatcagg tgaaagtctg
    19021 tatcttatga tatttttatt agaagattgt attccacaat agtagcaatt atttttacaa
    19081 tagtttgtaa gctctataag tcctctcaag tagactctat ttccataatg tttatttctc
    19141 atgtcgctag cttgtttata aagatagttt cttaaatttt gttcgaagtt atattcctca
    19201 ttgtcgatat tttctattaa atatagtagt tcatcaaaac ttaatgaatt aatgctgtaa
    19261 agtttatcta taaagtattt aatcttattt ttagtcatat tcaaatatgt aattattaag
    19321 tatagttaaa tttttaataa ttgaaaagtt caagtgatct ttccaaaata ccttttacat
    19381 atgctattaa gataccatag tttacaatag gtacattgaa agattttgcc ttttctattc
    19441 tagataacat tccagctcta ttcatcatac aagctccaca gtgaactaca agggcatatt
    19501 tatttatatc ttcagtgaag gatactccag aactaaattc aaaagtaata tttttgccag
    19561 ttttctttct aatcatatta ggaattttta ctgttccaat atcatcagtt tgtctatgat
    19621 gggtacatcc ttcagccatc aagattttat caccatcttt taaattttct aaagcataag
    19681 ctccattaat caattcttta aggtctccct tttgtctagc aaacaatata gaaaaagatg
    19741 taagaggaat atcttttggt gtatctttat ttacttgtgg aaatacttga gagtcagtaa
    19801 taacaagttt tggttttttg cctaaattag atagagtttc ttttaaacta tcttcttttg
    19861 taactataga aattgcacca ttatccaaaa tatctcttat aacttgctgt tgaggaagta
    19921 taagtctacc ttttggagct gctttatcta ttggaacaac taaaacaact aaatcatttg
    19981 gttcaattaa atctgatacc aatttgaact cagtgctatc cttaggcaaa acttttatta
    20041 tttcattttt taaattttca attccaattt tatctgttga tgagacagaa acaactggac
    20101 atttaacttt atttttagtt aagtttaaaa tttctgattg attcttaata gtatcaattt
    20161 tgttgagaat tagaatatga gggatattct tatcattaaa cttttcaatt agtgacaaat
    20221 cttcttgtga tataccaatt tggcaatcca ctactagtaa agctatatct gtcttttcta
    20281 aaacatctag tgatttacct attctaagtt caccaagttc accaacatca tcaagaccag
    20341 cagtatctat aagaacacaa ggtcctatag gaaggatttc cattggtcta aatactgggt
    20401 ctgtagtagt tccagcaata tctgagacta ttgcagcact ttgatttgtt atagcattta
    20461 ttatgctaga tttacctgca tttcttttac caaagagacc tatatgtact cttaccgatt
    20521 gtggagtaga atttagactc atttgtactc accaccttaa acttcttttt ttgatatact
    20581 agtttttaca gatacatgag gtatgttgcc caatttacca gtcaaactat taatagtatc
    20641 catttcgcca acaactgcta tacatacgat tgagacacct tcttcttcgc aaggaatgcc
    20701 cattcttcct ttaataatac ctttaaaacc tgatacaacc tcattgaatt taaattgaca
    20761 ttcttttggc tcttccaaaa tagcacttat aactgcaacc tttttcataa atatcctcct
    20821 aactcagaaa atatgtctta aacaaatcca aaatcaaaaa acttttcttt gtaaagaaaa
    20881 gtttctacaa aagaaaagtt attaattaca caagtataat gtgctatatt aaactatttc
    20941 cttacagatt agataactct tttctggtag ggttagcttg taaaaattta atttaacaac
    21001 aagtaactga agaaaatttt tatacaattt atataaatta taataaaaat taactaaaga
    21061 aaattgaagt tacttatata atagtagtaa attgaaatta agtctataga aaaaccaatt
    21121 tttgtataaa aataaaaatt aaagttaatt atttgttaag aattgacttt ggtaaaatat
    21181 ataattaatt attctttaag catataattt ataagtaatt ttagtgtatt ttcaatagct
    21241 tctatatgtg ttctttcata atggtgacta ttgtttgttc caggaccaac acatgctatt
    21301 tgaacatctt ctccccaacg aatagcttct gaagcatctg aaccatagaa agtaaataca
    21361 tcgacagcgt agtctaaatt attgtcttct gcaacatcga caagcttgtt tcttaaatta
    21421 aaatcatata atgataaatt atcttttgca actatagtta caccatgttc actagatgtt
    21481 tgacttggtc cagtaggtgc tatatctatt gctacaaatt cttttgtttt ttcaggtaaa
    21541 catttagaaa caccatgtcc aatttcttca taattactta tataaaagtt agtagtatgc
    21601 ttaggcgtta aattgttttc tttaaaatat ctacatattt caagaaccat tgctacagct
    21661 gatttattat ctatatatct agattttata aaaccagatt ctgtagcctc aaatctatgg
    21721 tctaggcata caaaatcccc aacacttatt cctaaatcta atacatcttg ctttgtaaat
    21781 actttttcat caagtctaat aatcattgtt tcttcatttc tttcagtggc agcttttgtt
    21841 tgaccatata tatgagtaga agcatactta aatacaacac ttcctctgat ttttttacct
    21901 tttctagtta taacagtgca gttttctccc tctatagctg cccaacatcc accaccaact
    21961 ttatggtatt taagagtacc atcagatgat atttctttta ctatagctcc aagtgtatcc
    22021 atatgtgcag atattgttat ttgatttgaa tcatcttcac caggaagagt agctataagt
    22081 gcaccttttt ttgtaagaat agtgtcaaga cctaaatttt caaaatcttt tttgatttct
    22141 aaaactgcat tttcagtata tccagctggg cttggaatgt ccaaatattt tttcattaaa
    22201 tttatagtat tagttaaatt taaattcata ttgtttcctc ctaaataaat atatttatac
    22261 aaattataac ataaatacaa atgttctgaa aactaaaatt aaaagaatag aatc
//
LOCUS       DAMKPG010000063        22242 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMKPG010000063 DAMKPG010000000
VERSION     DAMKPG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.066"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(327..1433)
                     /locus_tag="PC621_002459"
     CDS             complement(327..1433)
                     /locus_tag="PC621_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF4109606.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(1468..3009)
                     /locus_tag="PC621_002460"
     CDS             complement(1468..3009)
                     /locus_tag="PC621_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF4109607.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            complement(3371..4117)
                     /locus_tag="PC621_002461"
     CDS             complement(3371..4117)
                     /locus_tag="PC621_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4109608.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            complement(4329..5639)
                     /locus_tag="PC621_002462"
     CDS             complement(4329..5639)
                     /locus_tag="PC621_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4109609.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(5652..6122)
                     /locus_tag="PC621_002463"
     CDS             complement(5652..6122)
                     /locus_tag="PC621_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109610.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(6126..6977)
                     /locus_tag="PC621_002464"
     CDS             complement(6126..6977)
                     /locus_tag="PC621_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF4109611.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            7902..8813
                     /locus_tag="PC621_002465"
     CDS             7902..8813
                     /locus_tag="PC621_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF4109612.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            8988..9485
                     /locus_tag="PC621_002466"
     CDS             8988..9485
                     /locus_tag="PC621_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF4109613.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            10229..10591
                     /locus_tag="PC621_002467"
     CDS             10229..10591
                     /locus_tag="PC621_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF4109614.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            10657..11274
                     /locus_tag="PC621_002468"
     CDS             10657..11274
                     /locus_tag="PC621_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF4109615.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            11271..11756
                     /locus_tag="PC621_002469"
     CDS             11271..11756
                     /locus_tag="PC621_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF4109616.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            11799..12317
                     /locus_tag="PC621_002470"
     CDS             11799..12317
                     /locus_tag="PC621_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF4109617.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            12453..13412
                     /locus_tag="PC621_002471"
     CDS             12453..13412
                     /locus_tag="PC621_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF4109618.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            13438..15141
                     /locus_tag="PC621_002472"
     CDS             13438..15141
                     /locus_tag="PC621_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF4109619.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            15398..17500
                     /locus_tag="PC621_002473"
     CDS             15398..17500
                     /locus_tag="PC621_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF4109620.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            17513..17953
                     /locus_tag="PC621_002474"
     CDS             17513..17953
                     /locus_tag="PC621_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF4109621.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            17989..18792
                     /locus_tag="PC621_002475"
     CDS             17989..18792
                     /locus_tag="PC621_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF4109622.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKRVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            18832..19428
                     /locus_tag="PC621_002476"
     CDS             18832..19428
                     /locus_tag="PC621_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF4109623.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            19534..20397
                     /locus_tag="PC621_002477"
     CDS             19534..20397
                     /locus_tag="PC621_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF4109624.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            20464..21453
                     /locus_tag="PC621_002478"
     CDS             20464..21453
                     /locus_tag="PC621_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF4109625.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            21545..22156
                     /locus_tag="PC621_002479"
     CDS             21545..22156
                     /locus_tag="PC621_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF4109626.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8653 a         2619 c         3726 g         7244 t
ORIGIN      
        1 gagcttgtta ttaaaaatac tagaacaaat tattatgtaa aatactaaag cttgttatta
       61 aaaatactag aacaaattat tataaaaata ctagaacttg ttattaaaaa tgctagaaca
      121 aattattatg aaaaatattg aagcttatta tgaaaaatgt tagaacaaat tactatataa
      181 aataccaaag cttattatga aaaatgttaa aataaattat tatataagat actaaaacct
      241 atgtaaaaca actttaatat ataacttata aagttgaaga tacataggtt tttattttta
      301 taatttaaag aggtatatta tgatatttat aatttaatca tatgataaac tattttggca
      361 gtatcatata attcgtctat atttacatat tcattaactg tgtgtacatc atacatacct
      421 agacttaata tagcacaatt atatccaaga ttagctaata tatttgcgtc acttccacca
      481 ccaattacca ttggatttgg aacaacacca acttgtctta ttgcttcttc acttaacttg
      541 aaaacgtgac tatctctact aagttcaaaa ctagggtaag acatactatg ttcaaaggta
      601 taagtagtat taaatttaga tgcagcatcc ttacaacatt tttccatatg attaagctca
      661 tactctaaag tttcaggtat atgagaccta atttcagcag tcaatgtaac cttatcagtt
      721 actatgtttg tagctccacc accttctatt ttacctatat ttgaagtagt caatggatct
      781 attctgccta tgtgcatatt gcttattgca tgagaggcta ctaatatagc atttaaccct
      841 ttttcaggtt ctataccagc atgtgctttc tttccatgaa aagagatttt tatagattgt
      901 tgagcaggag ctttgtatgc tatcgaacca atagctccac cagaatctaa aatcaccata
      961 tctttacaag gcaagttgtc aggattaaag tttttgacac catgcatacc agcttcctca
     1021 cagattgtaa aacaaagata aatatctcta tgtggtattt tttctgtagt aacatgttct
     1081 aaagcttcaa gtatagcagc tattccagct ttatcatctg caccaagtgt agtagttcca
     1141 tcacttctaa ctacattacc atctagtatt ggttttacac ctaaacaagg ttgaacttgg
     1201 tccatgtgtg cacataaaca aagtggtcta cttccttctt caccttttat ataggcaatg
     1261 acattacctg tattaccacc atatttttca cctgcatcat ctataatggc atctatattt
     1321 ctttctttta agtagtttac caaccactta gacatttcta attcttgatt agatgggcta
     1381 tcaattctaa ccatatccat aaaattattt ataagtctat ttttatctaa cataaattat
     1441 ttctcctttt agaaagattt aaataaatta catgctatat gaaggaagta ttccaggtcc
     1501 tagtggaata ttaaatacca taaacaatat taatagtata gtccacacta ttaagaatga
     1561 tatagagtat ggtatcatat tagcaattac agtacccata ccagcatctt tatcgtattt
     1621 tcttgtgaat gctagtataa tagggaagta cggaaataat gggctaagtg gatttgttat
     1681 acaatcacct atacgataag ccatctgagt aagtgctgga ttgtatccaa ccatcataaa
     1741 cattggtaca aatataggtg caagaatagc ccactttgca gatgcactac ctataaataa
     1801 atttataaaa catgataata taataaatcc tatcaacaat aaaggtccat ttattcctgc
     1861 tgattttaaa aattctccgc cctttacagc tagtatcaga cctaaattac tatttgtgaa
     1921 taattgcaaa aattgtgaag cagcaaatgc tagtattata tatccaccca tatcactcat
     1981 tgcactagcc attatagaga ctacatcctt atccttcttt attttaccta tagtttttcc
     2041 gtatacaaca ccaggtatga aaaatgctag tgctattata ggtaccatac ctttcataag
     2101 aggagcattg tttgatagta aatctcctgt ttctgggtca gctaagaatg gttttttacc
     2161 gattacacta agagctacta taatagctac atatattaat atacttatac cagctttttt
     2221 aagccctttc ttttctacat cactaagact tccatcaaca tctagttctg catttccttc
     2281 atatttacca aatctaggag ctacaatttt ttctgttacc catgttgcca aaagtattag
     2341 aacaaaagtt gatgctatca taaaaaataa gttcattgtt gcattggctt gaaatccaga
     2401 atcaatcatt tgagctgctg gaactgtaaa acttgcagca agtatatcat tgacacttat
     2461 cataatgttt gcggcaaatc ctagacagac tgctgcatat ccagaaaata aacctataag
     2521 tggatgtcta ccaacaccaa gatatacaag tgcagctaat ggtggaaata atattgtacc
     2581 tgcatctgaa gctatattgg caagcatacc tatagtcatt acaacaagag ttactcttgt
     2641 ttcaggaatc ttgcttactg atgctttgat tgttgctgtc ataagtcctg ttttgtcaca
     2701 tacaccagca cctatcatag ttactagaac tagacccaaa ggagcaaacc cttggaaatt
     2761 gctaactatg tttcctaaaa gaatttgaag ctgttctaca gtcaataaat ttactgcagt
     2821 tatagtttct ccagtaccag gatgaactac tgatatcccc atagaaccaa ctagactaga
     2881 tagtataagt aaaagtacac ataaccctaa aaatattgta actgggtctg gtagtttatt
     2941 accaacagtt tctattctgt ttaatgtcct gtcaaaaaag tttttcttct taactacgtt
     3001 atcattcata atatcctccc taatgtttaa aaatataatt aaaaaataag tattgtggtt
     3061 gatcttacaa tataaattct gcttgtcgat ttaagacgtg tccacaatat gtcctaaaaa
     3121 aagtataaaa taaacttttt cctgtgtcaa tgcagttttc tgaattttct ggttgagtgg
     3181 tgttagttaa atatgcttta agtagattaa tgacgaatta tatctaatgt atttaaatag
     3241 catatgcact ttaaagttaa ttttaaaaca aaattttatg aaaagctagt ggaagattaa
     3301 gttattaaaa taagtagcgt agtaaagtgt tagatttttt aattaatcaa agaaatttac
     3361 tttgtatttt ctacaaaaat ctgtgaatat cttttgaatt atgtagagga tataaattta
     3421 ttttatttac atagattaga ttttgttcta atttactgta ttcaaatggt tttccatgtc
     3481 caggataaat cattttaggt tttgtattga tgattgtttt ccaagattga ataaattcta
     3541 gtttgctttc tgcaaatatg gtaattttgt gagtacttgg aaatccattc attgcagaat
     3601 ctccacaaaa taaaacacca tcatcaatta gtagtgaaat tgaatcagca gtatgtcctg
     3661 gtgtatctat aatttttcca tttagttctt tttcaatttc tttacgattc ttgtcagaca
     3721 caagaataca ccttttttca aattcttgtt taagtggaga aaaactatgc tcaccttttc
     3781 caaatagttt cataatttgg caaaagagaa aggcagtacg gctgttacaa ccaccaataa
     3841 aagagttttg acctttatat agaatttcta aagctttatc atgcataaca atctttgtat
     3901 taggagattc gtctagaatc tgatttagaa aaccaacatg gtcatcatga gcatgagtta
     3961 gaaatatgta tcgaatatct ttgattttta tgttgaattt agaaattcgt tttttgaaat
     4021 gtttaaaacc attttcatat ccagtatcaa tcaagacaaa gccatcttga atagggtaca
     4081 accatgtatt tataattcga tttcctgcat taaacatttt ttcaccacct tgaattagaa
     4141 tataaattta tattaattag aaagaatata gcatagttct actgatattt taacgtatct
     4201 ctaaaagttt taaggaggat aatatatttt agtttaatct aaaagctaaa agaaaagaag
     4261 tatactgtta caaaaacaaa gagtggtata acttttacag tagataccac cctattataa
     4321 aaatagactt ataaagtttt atcgtgtttt tctaggaatg ttagatattc ttctttagtc
     4381 ataactggag taaaggtatc taatatatct ttagcatcct tagcattatc aaaaagaagg
     4441 tctacaactg taagtgccat tgctttagca ggaattatgt aagctaaatc ttcatcaact
     4501 atttcaaaat ctcttgtatg aagagctcct tttacaccac caatcatagg gtgaagagta
     4561 ggcattatat gagacacatc tccaaaatca aaagagcctg taaaatctcc accatcaata
     4621 acttttcctt ttagacctaa atcttcaaga ttgtttttaa ataaattatc aagacttttg
     4681 tatcgtaata ttggaagata tccaggaatt tcagttatct caacatcagc tccaactgcc
     4741 attccaccag ccatcaaagc cttatttatt ctttcattag catcaatcat accatttata
     4801 gttctagctc taacatatga ttccatatga acatcagcag gaacaacatt tactatatct
     4861 ccaccttttg tgatgatagg gtggaatcta actctttcag actctttaaa agtttctcta
     4921 agtgcattca cgttgtttat agcaagcatt gctgcattta aagcatttat tccatcatgt
     4981 ggagcagaac cagcatgaga ttctttacct ataaatttaa ccttttttcc aacaaatcca
     5041 ttactctcag gaccaactag agccttatct tctcctaaat cagaagcatg aaacatcata
     5101 gatatgtcta tatcatcaaa ataacctctt tttattaatt cttgttttcc accaaaataa
     5161 gttatttttc cttctttttt taattgctct ctgtattcca actcaataaa ctcttcagca
     5221 ggagtagcca taaaagatac tttaccatct aaatgttcta aaacgccact ttttactaaa
     5281 ccaacagcag cacctaacat tccagctatt tgaatattat gtccacaagt atgagaagca
     5341 cctatttcat tagcatcttt atgttcttta catgatatac catccaattc tccaagtata
     5401 gatatatgag gacctttctt gctagaattt gcatctgctt tgcatcctgt aactgctata
     5461 tttttttcta cagataaatt taattcttgc tcaaaaaagt ttgaaacagc ttctgtagtt
     5521 ttaaattctt tatatccata ttctggattt ttgtatattc cttgacctat ttttaatatt
     5581 ttttctctat tttcatctat agtttttatt actagagatt ttaattgttc tttattcata
     5641 ataaattcct cctatatttt accttgaata cttaaaacta tatgagctat actcgcacat
     5701 gcaaagaata tagttgtaaa tacaataagt gaaattataa ctattttcca tgacatttct
     5761 tttaaagctt ctattttgtt tccaacagaa attcctgcaa aagctagtag tggagtagtt
     5821 atagccataa agttaatatt attagtgtta gttaagaata cttcagatgt tggactatat
     5881 ggcatactaa gtaaaaggcc gattatagtt gcaaatccaa aagctggaaa gattgattta
     5941 ggaaataaat cttttataat catagctgcc atagcagcta atattagcat caccatacca
     6001 ggaagagctg taactataga tgtaccaacg ctaattcttt gtcctataag aattataact
     6061 ccaataacag acataaccaa taattgatat gaacttttct ttaaatttaa attagcaaac
     6121 atttattagc actctccttc tgtagattta atttctttag tttttttatt tctcgaaaat
     6181 ttaggttcta aaatcttata cataaaatta gtaaatggta aggatacaaa aactaagaat
     6241 ataattcctg ttatactaga catcatattg cttgttgcag cataggcaag tattgtatct
     6301 gccatttcag gtggtaccat ggtagataat gaaccagcag ctgctgtcat catacttcca
     6361 ctaccaacac cacaggccat tcccaaagca tatgggtgaa gaccactata aatagatatt
     6421 gaacctagta cactaaagta tatagtacca agaactgttc ccattaaata tgtaccaagt
     6481 acaccactac cttctggtga gtttatccca tatttttctg atataactcc taaagaaggt
     6541 tcacgactta tacttgcagt agctccaaca gcttctcttt tcattccaaa cattaatgca
     6601 agaggtaatg caacgattgg tgcaagtata tgaccaaatt cttgagcgat aaatgctggt
     6661 ccagcttgaa ttattttgtc aatgtttggt ccaactgtag taccatattt tataccaagc
     6721 attactagtg caattccaac aacttcacca gcaatattta tttctttttc tccaaccact
     6781 tttttaagtg ctttaatttt tttacctaat aaatctggtg ttattataag tccaattata
     6841 actgcataaa gcatagggaa cagtactata gaggcttttc caatagctat agttctttgc
     6901 cctataaatt cacatattac taccaagatt aagcaagtta aaaatacttg aataaaatgt
     6961 tttttattaa tttccatgat ttcctcccca aaagaataaa tattttactt atctgaaatg
     7021 agaaaatacg ttttcttaag tataatacgt tttcctaaat aaaatgaaaa aactatttca
     7081 aataagtttc agaaatgtca ataacatgtc cttaattaag gataaaacaa aaatttaaca
     7141 ttgtcaacga aaaatatcta aaatattaca tattttgaat atattgtaat atagctttat
     7201 tatgaaaaaa ttgtttaaat attgtaggaa taatagctaa aaagctttaa aattagtaat
     7261 ataaaattga aaaataataa tatagagttg agaaatagta aattgaaatt ttaaaataaa
     7321 tgagtggaag tagcttagta aagtatggaa atgaatatta caatagaaaa taaataatat
     7381 gtaaagactt taaattaatt ttaagtatta agtacaaata ctaatttttg atatagaaat
     7441 tgaataaagt aaatgataat aagcaaatga attaagatga taataagtga attgatatag
     7501 aaataagtaa attaagtaag atgataacaa atgaaatgat aataagtgaa ttgatatagc
     7561 aataagtaaa ttaagtaaga cgataacaaa tgaaatgata ataagtgaat tgatatagca
     7621 ataagtaaat taagtaagac gataacaaat gagatgataa taagtgaatt gatatagaaa
     7681 taagtaaatt aagtaagatg ataacaaatg agatgataat aagtaaattg atatagtaat
     7741 aaataaatta agatgataat atccaaagtg agaaagataa tagaaagtaa ataaaatcat
     7801 gtaagaataa tttagataag attctaagat tttatttatt taaaaattag tatgattaat
     7861 ttagattaaa taaatttaat aattttagta ggtggtaatg aatgaaaata aatagattaa
     7921 cagaaataat agtaattcta ttaaataaaa aattagtaac agctaaagag ttggcagata
     7981 ggtttgaagt ttctacaaga accatatata gagatataga aactctgtct atgtcgggag
     8041 taccagtata tatgacgaaa ggcaaaggag gagggatatc tttaatagaa gaatattcta
     8101 tagacaaggc catactgtca aaaaaagata aagagagttt aatagttgca ctgaaaacat
     8161 tacaggcgac taagtatcca gaaataaatt cagtagtaaa taaaattggc tcaatatttg
     8221 gagagcagaa tttttcaaac tggattgaaa tagactttac tgaatgggga agtaatttta
     8281 atgaagatga taaatttaca aaaataaaag aagctatttt aagacgtaat actataaatt
     8341 ttaattatgt aaatagtttg tcaagccaga caaacagaac tgtcgaacca ctgaagctta
     8401 tgtataagag taagacttgg tacttgtatg gattttgtaa actaaaagat gattttagga
     8461 tatttagaat tagtaggata aggaatttat ctataaagga tgaagtattt tcaagaaaga
     8521 taatagaaga agtatgtttg aatgattcta aagtaattaa ggaaaatact ataacgttga
     8581 aattgaggtt taaagaaaag atgcttttta gagtatttga tgattttaat aaggatttaa
     8641 taactaaaaa tgaagatgat acatatgatg taattacaga atttcctatt ggtgaatgga
     8701 tttatggata tattttatcg tttggtgaca atgttgaagt acttgaacct aaagatgtta
     8761 gggataatgt aataactaga ctaagggagt tgtctaaaat ttattcatta taatatgatg
     8821 ctagattaaa gattttatta aatatataat atcttgcatt tagtatataa tatctcactg
     8881 agtttaatta tattaaaaat atgacataaa ggtgtcgtat attataaatt ataattgaat
     8941 tagttcaaaa taaaatatat taataaaaac aaagtgaggt ataaagtatg aattatgaaa
     9001 tagtagagat aaatgagaaa atagttatag gaatatcgaa ggagactaca aataaagatg
     9061 gacaagcggt aaatgctata ggtgaattgt ggaaaaaatt tatgggcaaa gggatatata
     9121 atgctataaa gggtaaaaag aatgacaaga caataggtct atatacagat taccagggtg
     9181 attttacaag tccgtatagc tttgttgcat gttgtgaagt aaatagtaat tctgataaag
     9241 agaagaattt agaagaatta aacactaata atactgttgg agaaagtatt attagtaaag
     9301 ttatactagc aggtaagtat gcaaagtttg ttatagtagg aggtcaaaaa gaagtaggag
     9361 atttttggtt taagttttgg caaatggatt ttgataggac ttatataagt gattttgaag
     9421 agtatcaatg taatactttt gatacaaaga aacaagaaat tcatatttac ataggaataa
     9481 agtagatttg ttattactat attgaatgaa gtattgagta tagtacattt attatagata
     9541 gttttactat ttataactat aagcagtaaa tctgtaaaag atttactgct tttttagtaa
     9601 ttaatagtat cagtatcact ttgaatcttc agacatattc gtcatttaat attgtttatt
     9661 gataaatgat acttgtaaat ataatgattt gttatttaat ttaaaatatg aaaaaaagta
     9721 ttataaacac attagaatac ttctggattt atgagtatac aatttgaaaa atataaaaaa
     9781 taatgtataa agctaaatct aatttaatcg ataatacata tagagaaata taattaagta
     9841 aataaacaac tatctattag atattgagaa agtcaaatcc tatatgaatc tacagaaatg
     9901 attttaatga atctgaaaaa attttttata caaacataaa aaaggtataa agttgtttta
     9961 ttataatttg attacttttg ataacttttt atgttttata caattatatt tttaataact
    10021 cgtcaaaacg ccaaacatat attacaatag atatataagt ttttttgttg acaaattatg
    10081 ttaagagaat taagtataat atggcataaa taattacttt tatttatcaa tgaaatgtat
    10141 ttattgtgta gcgaaaaata gtcagatagt atagacaata actgaagata aaagcgttta
    10201 ttgttgtaca tatggaggag ttagttttat ggaaaataaa attttgatag tagatgatgc
    10261 tatttttatg agaatgataa ttaaagaagc tctgagtaaa agtggctata ataatttaat
    10321 agaagcaaat gatggagaag aagcgtgtaa tatatttaaa tatgaaaagc ctgatttgat
    10381 gttgcttgat attacaatgc caaaaaaaga tggtttagag gtgctaagag aaataaaaaa
    10441 gttggattca agtgctaagg ttgtaatgtg ctctgcaata ggacaagaga atatgattgt
    10501 agaagccata aaacttggag cgttagattt tatagtgaag cccttcaaaa cagaaacatt
    10561 agttaaagtt gtaaataatg ttttaaaata aaagtgtaaa tatacactga tacaatgaaa
    10621 agcaaataaa tgcaataata aaagggaggg gtttatatta taaattattt agaacttgat
    10681 gatgttcata tagatgctct tagagaaatt ggtaatatag gctctgggaa tgcaattact
    10741 gcacttgcat ccatgataaa tagcaatgta gaagtattaa tacctatggt caagatactt
    10801 gaatataatg aagcgacaaa tttgcttggt ggtccagaaa acaaagtagt atgtatttta
    10861 ctcgatatga aaggtgatat caatggaatg tttatgtttt tgttggatga atcaataact
    10921 caacttatgc taagttctct ttttaataaa gaagaagctt ttttagatga gattgaggca
    10981 attgaaattt cagcaattaa ggaaatagga aatattatgg caagttcata tgtaaatgct
    11041 attgcatcta tgttaaacat gactataagt gtttcaattc cagatatatg tattgatatg
    11101 gttggagctg ttctaaatgt gcctatgata agattttcag atgttggtga caaagtctta
    11161 tttatagaaa ataaatttaa aatgagtgat aattatttta ctagccacat tttgatgatt
    11221 ccagaaatga gctcacttag agaaatactc gtgaggttgg gattagaagt atgaatagag
    11281 agatagtagt gaacattgca gatatgaaga tagcctatag accaaatgtt ttagttactt
    11341 atgcacttgg ctcttgtgta ggagtatgtt tgatagataa agtagctgga attggtggaa
    11401 tgttacacgt tatgttgcca tatagtaaag atgctattaa cattgaaaat aagtgtaaat
    11461 ttgctgacac aggaataaat gagctcatta aatctatgga gaatataggt gcaaatagaa
    11521 tatatataaa agcaaaaatt gctggaggtg ctcagatgtt tttaggaagc agcaattcag
    11581 taattgcaag tataggacat aggaatgttg aggcagtaaa aaaaactctt ttaggattaa
    11641 atattccaat attagctgag gacacaggta tgaattatgg tagaacaata cgtttttaca
    11701 ctgagacggg tgaattactt gtagattcaa taaataaagg taagaagaaa ttataatata
    11761 tatttcatag aaagtgtatt ttaggaggga ggtattaaat gagatttcca gatgacccat
    11821 atttagatta tggactaatt gatgaaggaa ttaagtatct aaagtttaaa gtagatgagc
    11881 aagattttgg ggttgaactt gaaaaggtaa tagaaattac tggaaatcaa gaaattatat
    11941 ttattccaga actacctaca tattctaaag gcataataaa tctaagagga aaaatcattc
    12001 caataataga tatgcgtttg agacttaaaa aagatgaatt aatgactaca aattgcatgt
    12061 atatagtagt aactgaaata aaggacttgg tagttggatt tgtggttgat agagttgatg
    12121 atatagtaag cttagaaaaa agtaaaatat cacctccacc aagagttact atagaatatt
    12181 cggattattt tgtaagtggc gttggagaat tagaaggtaa aataattact ttactagatt
    12241 tagagaaact tttgacagat aaagatgaag ttttaataga tgaattaatc aatgggtttg
    12301 attgcactat catgtaacat agggatgtag ttggagtgtt attataaaaa gattgtgatg
    12361 agaacattta agaatatagt ggtataatta ttaagtataa aaattttaaa tcattaaaat
    12421 ttgaatatat tatgtttaag agaggtttgt atatgaaaaa taagcaacag ggaacaattt
    12481 tagtcgttga tgatagtgtt ctgatgtgtg acttaataga taaaacactt acaaaagata
    12541 attttaaggt taaaaaagca tttaatgcaa tagaggcaaa agaatttatt gaagaattta
    12601 taccagacat tattttgctt gatataattt taccagataa ctctggtttt gagttttgta
    12661 aggaagtaaa atctaatact cgtacagcag atatacctat aatttttata acttcaaaaa
    12721 atacagatac agacatagta aaaggatttg gtgtaggagc aattgactat atggtgaaac
    12781 cttttagtat gacagaatta aaagctcgtg ttatagctca tcttgaagta aagaaatctc
    12841 aagataaatt aaaaaagata aataatgaac tggaatcttc tcttgaaaaa ttgaatagac
    12901 tcgttgtaag agattatctt acaggtcttt acaataggcg acatataatt aatcagctta
    12961 tggagcaaag aagaattaat aaatatagtg aaacatcaat atcccttatc cttggtgata
    13021 ttgatgactt taaggttatc aatgatactt atgggcatga agcaggggat ttcgtgttaa
    13081 cagtaatatc aagtataata aaaaagaatt gtagacaaat tgatactgta tcaagatggg
    13141 gtggagaaga gtttttgata atgctcacaa atactccaat agaaggtgct aagatacttt
    13201 cagaaagaat ccgtaaagag ataaaggagt ttgatttcaa ctataaagaa cataaaataa
    13261 agtgcactat tacattagga gtagtagaag taaactcaaa tatatcatta gaagaaaaca
    13321 tatccttagc agataaagca atgtatgagg gtaaaaactt aggaaaagat tgtagcgtag
    13381 tgagtacaat gaaaggatta aaaaagatat aaacttactt tatgaaggag tacagatatg
    13441 aaaagagaag taaagctttc taaattagca attgtattgc tgattattat agtattggct
    13501 gttctagcag ggattttttc ttctatttca tcttataaag catttgaaaa agtagaaaaa
    13561 agtattaatg atatgaataa tttgaggact caaagtcagt atataaaaga tacaactaga
    13621 gagataagtg atgtattaag aagatatgta tttacaggag aaaaaaagaa taaagatatt
    13681 tatgaaaaag aattatacgg aaaaacaaga gaatcagctt taaacaaatt agaaaaaatt
    13741 ggtcttacaa agagcgagtc aaataaaatc ttagaagcaa aagaaatatc agattcttta
    13801 gtaccaacag aacaaagtgc aataaaggca gtcgaaagta atgataagag taaagctcaa
    13861 agtataattt ttggtagtga ctatataaac tcccaagaga aaatggattc tttaataaat
    13921 gaatttcaag aggatatagt tacccgttca aattccaaaa ttgtagaagt aaaaaaagaa
    13981 ttacaaagtc taatattaag aacaatattt atactttccc ttttaataat attgacaata
    14041 atagaatata cagtaataaa attaaagata gtgattccta ttaatagagt agaaaaacac
    14101 ttttcaagaa ttgcatctgg ggacttaaga acaagtatag acgttgaaga aagtaaatct
    14161 gaaataggac tattagtgag ttctgtcaaa aagatgcaaa gtatgctttt aaattacata
    14221 gaccttatag acagtacatt aactagtatt gcaaaaggag atttaagagt agagatagac
    14281 caagaattta taggtgattt taaatcaatt aaaatatctt tagaaagtat aataaactct
    14341 tttaatggtg atttttctga aataaattta tcagcagaac aagttgcaag tgcttcagaa
    14401 caagttgcag caggtgcaca agctctttca cagggtgcta ctgaacaagc tagttcagta
    14461 gaagaattag cttccacgat taatgagata tcagatggaa ttaagaagtg tgcagatagt
    14521 gctagtcttg catgtgaggt atctgaccaa tctgcatcac aagttggttt tggaagtaga
    14581 tgtatggatg atatgatgac atcaatgggt gaaataagtg aaaaatcgag tgaaatatca
    14641 aaaataatca aaacaataga tgagatagca ttccaaacta atattctcgc attaaatgca
    14701 gctgttgaag cagcaagagc aggtcaatat ggtagaggat ttgcagttgt agcagatgaa
    14761 gttaggtctt tggctggtaa aagtgcaaaa gcagctcaaa atacagctat tctcatagaa
    14821 gaagctatta aaacagtaga aaatggtgtc agaattgcta aagagactgc aaatgcattg
    14881 gacttggtag taaatggtgt agaaaaaact actaattata ttaatggtat ttcttcggct
    14941 gtacatagtc aagaagtagc aattacacag attttatctg gaatagagca aatttccata
    15001 gtagtccaaa tgaactcagc aacagcagag gaaagtgcag cagcctcaga agagatgtct
    15061 gctcaagctc aaattttaaa agaattagta gaaaaatttc atctaaagga aagtgaagta
    15121 agagaatcat catgggttta aattatgagg tgaatattga agtaagagaa ttatcatgta
    15181 tttaaattat gaggtgaata ttgaagtaag agaattatca tgtatttaaa ttatgagatg
    15241 tatatatatt gaaataagag aatgattgtg tatttaaatt atgaggtaca tatagtgaga
    15301 taagagaatc atcaaggatt taaattatga gatgcatata taatagaaaa ttatttgtag
    15361 gtcgtaggtg ataaatgtga attatattga accgagtatg agacaaatgt tagatatgtt
    15421 tatatttgag acaagtttat tactcgaaca attagatgaa attatgattc aaatagaaaa
    15481 agagaaagta tttactcata acaacattaa tgaaactttt agaataatgc atacgataaa
    15541 aggttcagca tctatgatgg ggttagacaa tatatctgct ttagcacata gtgtagaaga
    15601 cttgttttat gctatacgtg aaggtaaaat agaagtttta gacaatgacc tattatttga
    15661 tttagtcttt caatcattag attttataag aagagatata gatgagctac aatcaagagg
    15721 ttgtatatct tctgattact ctaattttat tgaaggaatt gaagagtatg tgagtacttt
    15781 ggaattatca agttctatga aatcaaagga taatgcaaat ttagaaagta cagacgaaaa
    15841 agaagcaatc gattcaaatt ttgaatacaa agaagactct gatgtagtaa aaataatctt
    15901 tgaacaaggt tgcatgatgg aaaatgtgag agcgttcatg cttgtatttg aacttagaga
    15961 ttactgtgaa tttatagatt tttatccaaa agatgtagaa acaaatgcag agtctacaga
    16021 atatattaaa agttatgggt tttatataca atttgtagct aaagaggata aggcaatagt
    16081 ttataaaact atagaagata atctctatgt aaaagaatac ataaatatta gtaaaaaaga
    16141 aatagaaatg aaaaatacat caaataatga tgcagtagaa aataaattaa aagataataa
    16201 taaagtaagt aaaagttatc aaaataacta tacagaagat gagatacaag aagatgtttt
    16261 aaatgaaaaa actgatatgg aaaaagatag tctaagtgaa gaaaatgaac atttaccaat
    16321 aagtaagcag agtattatga gtgtaaattt agaaaaattg gatttacttc aaaatatagt
    16381 tggagaaatt gtaattatgg aatcaatggt tgtaaatagc gtaaattcta caggtgttga
    16441 tatagataat ttcaataagg caacaagaca attacacaag cttacagatg aattgcaaga
    16501 tatagtaatg tctatgcgta tgattcaaat tgctggtgta tttcaaaaaa tgcatagaat
    16561 tgtacgagat atgaataaaa aattagataa aaatgtagag ttaataacta taggagcaga
    16621 gacagaggta gataagaatg taattgataa tttagcagaa ccttttatgc accttataag
    16681 aaatgctatg gaccatgcaa tagagcctat tgaggagcgt atagcgaaag gtaaagaaga
    16741 agtgggaaag atagttttat ctgctgagaa tctaggcgga gaagctgtga taagtatttc
    16801 agatgatggt agaggcatta tgagagataa gattttggca aaagctaagg aacaaggtct
    16861 tcttaaacat caaattaatg agtatactga tgacgaggtc aattctttga ttatgactcc
    16921 aggtttttct acaaaagaat ctataactga attttctgga agaggagtag gcatggacat
    16981 tgttcgtaaa agtattgaaa aagttggagg ttcaattgaa gtaaaaagta aacaggatga
    17041 agggacaacc tttactataa aaataccatt aactctttct atagtagagg gtatgaagtt
    17101 taaagttgga gattcactat ttactttacc aatatcatcc ataagaagaa catttaaact
    17161 tacagactta aatcaaataa ttacagaagg aaataagaca gagttaatta caatatatgg
    17221 agcatgtatt cccattataa ggttacataa aatatataat attcaaacaa aagtaaccaa
    17281 tctgatgcat ggtaatatga taattattga aaatgagcat aaagcagtat gcatttttgt
    17341 tgatgaaata ataggtaatc agcaagttgt tgtgaaacca tttccagttt atttcaaccg
    17401 atttaatata aaaaataagg gtttatcggg atgtacaata cttggggatg gaagtataag
    17461 tttaattata gatgcagata gcattataga aatatattag gggatttaaa gtatggctaa
    17521 agatgaaatt attaaacaag tattaacatt ttatgtaaat gatgttattt atggaataga
    17581 attagaaaat gtgattgaaa ccataagatt tcaatcaata acgtatgtac catgtcttcc
    17641 aacatatata agtggagtta ttaatttgag aggaagaata ataccagtta ttaatatgca
    17701 tataaagtac aatcttccta aagctgatta taatgagaga acttgtataa taattacaaa
    17761 ggttgatgag tatcaagttg gaattattgt agataaagtt gttgatgtta ttcatataga
    17821 aaatctaaat ttactagaga cgactaattc taacaacact aatattaata aagatataaa
    17881 agagattgcg aaagttgaag ataattctat attaatttta gatattcgaa agtttttgat
    17941 taatagcata tagttagttc tgataaatta gcaggaggaa gtttttgcat gataaaactc
    18001 acagatgaag aatttatagt tcttgtaaac catgtaaaaa aagaatatgg tattgattta
    18061 agtaaaaaaa gagcgttaat tgaaggtcgc ttatacaata caatgataga gaaaaaactt
    18121 agttctttta gtcaatatat gaatctatta tttaaagata agacaggaaa tgaagctata
    18181 aacctaataa atagattgag tactaaccac acatttttta tgagagaacc tcaacatttt
    18241 gagtttatac agaatagtat acttcctttt tgggaagaaa ataataaaat aagaaacata
    18301 aatatatgga gtgcaggatg ttctagtgga gaagaagcgt attcaatagc tatgactttg
    18361 gatgattatt ttggatataa taaagagttg tggaacatta aaatcatagc cacagatatt
    18421 tcaactaact cacttgaaaa agctaaaaga ggtatataca tagaatcaaa tgttaacaat
    18481 gttcctgagt tatggaagaa gaagtatttt atagacaaca aggatggtac atttaaaatt
    18541 tgtgataaga ttagaaaacg tgtaattttt aagtcattta atttaatgaa tgatttctca
    18601 tatcaacatt ttgatttgat tttttgtaga aatgttatga tttattttga tttaaaaaca
    18661 agagaagaac ttataaataa attttataat gctaccaaaa aagatagttt tttgtttgta
    18721 ggacatgcag aagtaatcaa cagaagtagt actaaataca aatatgtaaa acctgctata
    18781 tataaacgat aaaaaggtat ttttgatata tgattttttg gaggcggtaa tgtggaacgt
    18841 tttaataata tggttattgc tataggcgca tctgtaggtg gtacagaagc aatacttgaa
    18901 attataaaag acctaccaaa atcaacacca gggattgttg tagttcaaca tatgccagct
    18961 atatttactt atatgtatgc acaacggctt gacaagcagt gtattatgaa tgtaagagaa
    19021 gctaagaata atgatagagt cgaacaagga aatgtgttaa ttgcacctgg gggttaccag
    19081 atgaaattgt gtacggataa acaaggatat tatgtaactt gtgaaaaagg agaaagaata
    19141 agtggtcact gtccatcagt agacgttttg tttgattcag tagcagaagt agcaggtaaa
    19201 aattcaatag gaataatact tacaggtatg ggttatgatg gagcaaatgg acttttaaaa
    19261 atgaaagaaa atggtttatt tacaattgga caggatgaaa attcttgtat agtatatgga
    19321 atgccaatgg tagcttttga tagaggttct gttatgttac aattacctct tggtgaaatt
    19381 tcaaactgtt tgataagaca tattaatatt ttaaaacaaa aagattaatt ttttataaat
    19441 aaaaattaat cttttattaa attatatgga taaaaaaccc tagaaactcc aaatataata
    19501 agagagattt atatttagga ggaaaatgat aatatgaaat tttttaaagt cgatacaaaa
    19561 gctaaagttt taagatatgt atttgtttta acattgacta taatggctgt tttggcaatt
    19621 aaacatgact ataaatataa taatttaagg aaagatgctt caattaattt aaatagtaat
    19681 aaggaagcag tgactgttat gagtaaccag tcattaccaa agggtaatac aaatagtaaa
    19741 caaactttat cagtaagtac aataggaagt aaaacaatag tgaaaaatga tgagtattta
    19801 gaaagtacta taaacatgcc tgtaataaca aattcaaata aaattgttga aaggtctaca
    19861 aatgataaga taaaaaatga catatttgaa ttttataata aatcatataa agaagcaaag
    19921 cagtatctta aggataatcc tgacgagaaa aataagtttg ttgcaaatgt agactttgaa
    19981 cttaaaaaga atactgattc tgctcttagt ataaaagtga gatattatac ttatagtggt
    20041 ggagcacatg gattttatca agatatagca tataatgtgg atatgagaac tggaaaattt
    20101 ttagaattaa tggacttatt taaggataat actaaatata aagaagttat agatgaagaa
    20161 attaaaagac aaatagctga acttgaaaaa aaggatgaag aaaatatcgg aatatataat
    20221 tttaaaggta taaaagagaa tcaaaacttt tacttgcaag atgagaatct agttgtatat
    20281 tttgatttat atgatataac tccatatgca gcgggaatac cagaattttc aataaataaa
    20341 gcgttgataa gttctatgct aaaaccagag tatgtagatt tatttgattt gaaatagaag
    20401 attaaattaa atgttatgct ataatattta gcatagaaac ttattaaaat tgggggtata
    20461 aaaatgctac ttttaaaaaa agacgacata aaaaaagtat ttacaatgag agatgcaatt
    20521 gaagctgata aggaagcttt tagaatttat tgtgaaggaa agagtgtaaa tcctttgaga
    20581 accaatatat ctgttccaag tcaagatgct agtatgttat ttatgccagg atacgtagaa
    20641 gaattaggct gtgggggaat aaaaattgta tctgtatttc ctaaaaatgc tcaaaaaggc
    20701 aaaccagtaa ttccagcaac agtactatta ttagatggag aaactggaga agttagtgca
    20761 gtattggatg gtacttatgt aactcagatt cgtactggag cagcctctgg agcagctata
    20821 gatgtattgg ctaaaaaaga ttctaagatt ggagctttga taggtctagg aggacaaggt
    20881 gagccacaac ttgaagccat gatagaagct agaaatttgg atgaggtaag agtttttagt
    20941 agaaacaggg aaagtagaga aaaatttgca gaagaaatga acttaaaatt aagtaaatat
    21001 aatactaaaa tagttgcagt tgaatcatct gatgaagcca tagataacgc agatgtgata
    21061 gttttagcta ctccatcaaa acaaccagta cttaatggaa acttagtgaa aaaaggagct
    21121 ttaataagtg cagtaggttc atatatgcca gatatgcaag agttgtgccc aaactgttta
    21181 actagagcgt ctaagatatt ttttgaatca acagatgcag ttttatctga gtcaggagat
    21241 atattaattc cattaaaaga aggtaagata agtaaagatg attttagtgg tgatttagga
    21301 aacgttataa atggaactat tcctggaaga gaagatgatg atgagataat tgtatttaaa
    21361 actgttggga taggtgtaca agatgtcgta acagcaaaaa gaatatacga taaagcaaaa
    21421 gaaaacggta ttggaataga ctggaagtgg taaaatattc aaaataattc tatagttaga
    21481 caaatttatg ttataatgtt gataaataaa ttttttatta ttttaattga gggaggatgt
    21541 ttgtatgaaa atggtaaaaa agttaatatt gtctacgatt atggctctag ttttagttct
    21601 aactataggg tgttctaatg cagaagaaaa agtaaagtcg aattttgaag ctaatttagc
    21661 tgcattagaa atgtataagt atgatgaaaa aggaatttct gatgcattaa cagaagctga
    21721 atataaacag attcaaactt ataacagcaa ggacaaggtt tcaatgattg ctcaattgac
    21781 acctctttat ggtaaagaaa atgctgaaaa gatatacgaa acaattgtta aattaattca
    21841 aggtgttgaa aagacagtaa cagtggaatc ttctagtaag gataagataa ctttgaaagt
    21901 tgtaagtaaa gctttagata ctagtaaagt aatgagtgaa gcgaacactt tagtaaaatc
    21961 ttatgtaact gatgaaaaga attacgataa aaaagaaaaa gaagttcaag atgatatgac
    22021 aaaaatgatt atagaccttt acaataagaa accaactaag gaagttacaa ctatgattac
    22081 atatactaaa aaagatggta aatggcaacc acctagtaat atacaaaagt tatgtgaagg
    22141 aatatataca atttaattta ttttaaagac acaaaactat cttttaagca tataataaaa
    22201 tgtttggaag atagtttatt ttattttcaa agtataaatt at
//
LOCUS       DAMKPG010000064        21951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMKPG010000064 DAMKPG010000000
VERSION     DAMKPG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.067"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(189..896)
                     /locus_tag="PC621_002480"
     CDS             complement(189..896)
                     /locus_tag="PC621_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF4109627.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(1095..3008)
                     /locus_tag="PC621_002481"
     CDS             complement(1095..3008)
                     /locus_tag="PC621_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF4109628.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3092..4183)
                     /locus_tag="PC621_002482"
     CDS             complement(3092..4183)
                     /locus_tag="PC621_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF4109629.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4189..5532)
                     /locus_tag="PC621_002483"
     CDS             complement(4189..5532)
                     /locus_tag="PC621_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF4109630.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            complement(5510..6262)
                     /locus_tag="PC621_002484"
     CDS             complement(5510..6262)
                     /locus_tag="PC621_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4109631.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6310..7218)
                     /locus_tag="PC621_002485"
     CDS             complement(6310..7218)
                     /locus_tag="PC621_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4109632.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7240..7989)
                     /locus_tag="PC621_002486"
     CDS             complement(7240..7989)
                     /locus_tag="PC621_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4109633.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(8018..9193)
                     /locus_tag="PC621_002487"
     CDS             complement(8018..9193)
                     /locus_tag="PC621_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF4109634.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9208..10047)
                     /locus_tag="PC621_002488"
     CDS             complement(9208..10047)
                     /locus_tag="PC621_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF4109635.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10050..11207)
                     /locus_tag="PC621_002489"
     CDS             complement(10050..11207)
                     /locus_tag="PC621_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF4109636.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11223..11945)
                     /locus_tag="PC621_002490"
     CDS             complement(11223..11945)
                     /locus_tag="PC621_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF4109637.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11957..13030)
                     /locus_tag="PC621_002491"
     CDS             complement(11957..13030)
                     /locus_tag="PC621_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF4109638.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(13052..14758)
                     /locus_tag="PC621_002492"
     CDS             complement(13052..14758)
                     /locus_tag="PC621_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF4109639.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(14800..16359)
                     /gene="murJ"
                     /locus_tag="PC621_002493"
     CDS             complement(14800..16359)
                     /gene="murJ"
                     /locus_tag="PC621_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF4109640.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            complement(16474..17529)
                     /locus_tag="PC621_002494"
     CDS             complement(16474..17529)
                     /locus_tag="PC621_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF4109641.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17624..18310)
                     /locus_tag="PC621_002495"
     CDS             complement(17624..18310)
                     /locus_tag="PC621_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF4109642.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18530..20554)
                     /locus_tag="PC621_002496"
     CDS             complement(18530..20554)
                     /locus_tag="PC621_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4109643.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20808..21866)
                     /locus_tag="PC621_002497"
     CDS             complement(20808..21866)
                     /locus_tag="PC621_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF4109644.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
BASE COUNT         6847 a         4055 c         2293 g         8756 t
ORIGIN      
        1 tgatatttga ttataatatg aaataattta ttataaaaaa aacagcaaag aaatttatct
       61 tctttgctgt ttttataacg ttcatcacta ttttaatatt actaaactct taaatctcta
      121 tgtaaaacat taaatgtaat tatatattta atgttaatat tcaatcctat gtaaatcaat
      181 atctattatt attgtaaaac acgtgtagca gttataagtc ttcttgcata attagttcct
      241 ggagccatgc tagtaacttt tactacatct cctgtttgtg gtgaatgtat gaatttagaa
      301 tctcctacat acaatccaac atgatttatt gaaccacctg aaccaaagaa aactaaatct
      361 cctggtttta attctgctct tgatactttc tttcctactg ttgcttgtga tcttgatact
      421 cttggtaaat taacccctgc tccattttta tagacataag aagtgaaccc agaacaatca
      481 aaagcatttg gtccagttgc tccccatcta tatggcttac cttgttgctt aaatgctaag
      541 tttaaaacat tttgtacctt tgcatctaca tttccacctt gaatagttaa ataatctcca
      601 tttacccatc ctgtaacatt gtcaacactt tttattttaa tccatccatt tgaatgtaat
      661 tctaaaactt caactttgtc tcctgtgttt atctttccta ctactgcatc atttgcacta
      721 gctccacttc ttacatttaa aaaatcacac tggtctactg ttgcactcat aggtgaatta
      781 gttggctctg ctgcactagc cgttattgct cctgcttcta ctgtgacaac tcctgctatt
      841 aacaccatag ctataaattt tttcaatata tgttttttat ttttaatatt catcataatc
      901 tcaaatacct aatcctttcg aaaatttaac attttgttcc ttcgtttcca attcctaatt
      961 aacattctat cttacatcaa aataaattgc aacctttttt gtaatctttt tgtaatcttt
     1021 ttgtaatctt ttgtttcttt ttagtatata tttgtttctt tttgctcccc ctggttacaa
     1081 attagaaata tattctactt ttctctagtt gttgcaattt ctttgatatt agcatcttct
     1141 ggtttaatat aagttggcac taattccctc ataataccat ctattttttc aacctcttca
     1201 ttgttagtaa tttctcttaa aagttttaag tattttgtta ttttttctct atcaacatct
     1261 attggtctgc caataaaaat ctttttatgc tctgtatcca acagaccttc ttctgacata
     1321 agtagttctt catacaactt ttctcctggt ctaagaccag aaaattcaat ttttatatca
     1381 acatctggct caaatccact gaatttaatt aagttttttg ctaaatccaa tattcttaca
     1441 ggttctccca tatcaagcac aaatatttct ccacctttag ccatagcccc agcttgtata
     1501 actaagccta ctgcttctgg tattgtcata aaatatctaa tgatatctgg atgagtaact
     1561 gtcactggtc cgccatcttc aatttgcttc ttaaaaagtg gaattacact accattacta
     1621 ccaagcacat ttccaaatct aacagcaaca aattcagtct ggctttcagc attcatagtc
     1681 tgtataatca tttcagcagc tcttttagta gctcccatta tgttagtagg gtttacagcc
     1741 ttatctgtag atataaggac aaatttctta gctctatatt ttgaagacaa gccagctatg
     1801 tttaaagttc caaatatatt attctttata gcttcgccag ggctcgattc cataagagga
     1861 acatgtttat gtgctgctgc atgaaatacc acttctggtt tatatttatt aaatatttca
     1921 tccattcttt tttcttctct tattgatgct attactgtct ttaaatctaa tttattttta
     1981 tattttctaa gtaattcttg ttgtattgag taagcattat tttcataatt atcaactata
     2041 ataagctctt taggattaaa tcctgcaatc tgtctgcaaa gctctgaacc aattgagcct
     2101 cctcctccag ttattaggat tacttttccc tcaatgtaat tacttatctc tcttaaattt
     2161 gtttttatag gctctctacc caacaagtct tctatctcta cttctctaat ctgttttata
     2221 tcaacttttc catctataat ttcgtaaatt cctggtattg tctttatttt acagttagtg
     2281 tttttacata tatcaataat ctcttttttt ctacgtttct ctatgtttgc tattgaaaag
     2341 attatctcat ctacattgta cttttctacc acagctttta tatccgaagt tgttcctaca
     2401 attttcacat tatggattat tcttccaatt ttatctttat catcatcaat tatagccact
     2461 gctacttttt taagttctga atttcttttt agttcctgta taaccatttc tcctgcatct
     2521 cctgcaccaa ttataaggat atttgaaacc tttcctctag aatatgtctt gttcatgact
     2581 attcttatag ctctataagc tattcttgcg ccacttgtaa acattataat aaatattgta
     2641 tttaatacat aaaacattat aggataatca aaccctaata atttatttat tatataaatc
     2701 ggaattaaat aagctaaagt tgccacaaaa atagatataa tttcttcttc tcctgcatat
     2761 ctccataatg tgttgtagca cttaaacaat ctaaatgata ctatatgaag tactatatat
     2821 atacacactg aaattattat atcattagtg gtaaatgcat ttaaattacc tgtcaagctt
     2881 aaagacattc gaaacgctac tagtacgcta caaatatcta gaattaatag tgttaaatgt
     2941 ctaaatttat attttttaaa aagcctatta aaaaagcttt ctttctctaa atctatactt
     3001 tctcgcataa ctagaagccc tcctttaaac agcaaaagct acgcacctaa gcatagccaa
     3061 caacatctat cttttgctgt attttttcat tttatttttt atatttatca aatatagaat
     3121 cataaatttt ttctatatca ttaaagttta aattaacctc ataattttct ctaacaaatt
     3181 gacgcccttt cttacccata ttaagtctaa gttcatcatt ttccattagt tttattatag
     3241 cattttttat ttcttctggt tgttttggtt ctacaattaa gccagtttca ttttcccata
     3301 cagattctgg atgtcctcct acatttgtaa ctataacagg tacttcacat gcttctgatt
     3361 ctatagctgc cactccaaat ccctctctta aagaaggaaa tacagttaca tcaaaagaat
     3421 taaaagtctt gcttacattt tctggagata ttcttcctaa aaactgaaca tcattttcta
     3481 tacccaattc tttagctaaa tttattaggt tgtccatctg acttccagag ccacctattt
     3541 taagaattat tttcttatct ttatattcat ctttaagcat cttaaatgct tgtattaaat
     3601 attctattcc atattttttt tctaaactct ttataattcc tattacaaaa gcgtcttctt
     3661 tttctacttc cataggctta aacctatcca tatctactcc aaaaaaggtt acatctacat
     3721 gtttagctgt atatttatta gcctctttag ccatatctct actatttgaa aagatatagt
     3781 ctgcttttct aaagttatgt tttataattt gtttttgtat aaaaccatta ttagggaaat
     3841 cataaatatc agtaccccaa acagaaataa tatatggatg atacccaagt agacttccta
     3901 taaatccata actacttgca tactgtgcat gcaatatatc tggctttatt tcatttacta
     3961 gcttctttat ctgctttatt gaacctaagt atcccgtttt tttagaaata ttttcatttt
     4021 ttaattcttc tacattagaa ccaaaattat aaatttttat tccttccatg tgacctccat
     4081 ttaaagagat tacatgaatg tcatatcctt tatttttaaa aaaactacac cattttaaag
     4141 tatgaggatt tgacccatca gctaaataac aaacagtctt cataatattt accttcctat
     4201 cgaataatac tcaattccta actcttccat atctttaatt ttataacaat ttcttccatc
     4261 aaggactaaa ggatttttca tcttttcctt atacttatgt atatcataat ttaatacatc
     4321 tttccattct gtcattataa aacatatctc tgcatcttct atcgcatcat ctacagaatc
     4381 agcataaagt atagagtcac caaatatttt cttagtatta tttacaccaa ttgggtcgta
     4441 tacagtaact cttgcccctc tttcaagcaa atattttata tttggtatag atggtgcttc
     4501 tcttatatca tcagtgtctg gtttaaaagt caaacctaaa acagcaactt tcatatcttg
     4561 aaagctctct actaacttag atgcctttct aactaattta agtttttgac tttcattaac
     4621 ttctattgca gcttttactg tttttaattc ataaccatta tgttcagata accagtgaag
     4681 agcttttgtg tcttttggaa agcaagaacc gccataccct attcccgcct ttaaaaactt
     4741 acttcctatt ctatcatcat aactcattcc taaagctaca tcttctatgt tagctcctgc
     4801 tatttcacaa agatttgcaa tatcattcat aaatgatatt ttaagagcta agaagttatt
     4861 tgatgcgtat tttatcatct cagcactatt tctatttgta actattatag gttgattaaa
     4921 aggcttatag atttcttcta atatttctct agcccattca ctttctacac ctataacgat
     4981 tcttgaagca tatagagtat cttttactgc tgttccttgt gataaaaact ctggattaga
     5041 tgcaagctct acttttacat tattttttag gtttctattt atataatctt ctatctcttc
     5101 atttgtacca attggaactg tagattttat tactactaga gtatcttttg tcacagtttc
     5161 agctatttgc tttgcaactg cctttatata atctaggttg gctgagccat cacttctctc
     5221 aggtgtacct acagctatta atattacatc tacatcttca taagcttttt tatagtctgt
     5281 agtgtaatct attcttcctc tgttgtaatt tttaaccata agttcttcta ggccttcttc
     5341 atatataggt gctattccaa gtttcattga tttaactttt tgttcatcta catcaacaca
     5401 gactacattt tcattgccta cctctgctag acaaacacct gtaaccaaac ctacatatcc
     5461 cgtcccagct atagctattt tcattttttt acctccaagt atataaaact tatactcttt
     5521 ttttaatggc attaaaaaca tagaaacaaa atacatatag actttttggt aaacttaaat
     5581 tttcaacatt tctataagta ttccaagttc gttttaatgc ttttatcttg ttacttgata
     5641 tagaatttcc aactaatctg taattcacta aatcctcctg aatcccatat gcatattttt
     5701 cctttttaag taactgtaac caggtcgcag tatcttgtcc tcttcttacc aaaggcatgc
     5761 tgaactctcc tacaatctcc ttatcaatga ccactgtaga acatcctatt atagtatttc
     5821 taagtaatcc ttcatagtca attttcttag gagctcttgc aaccttattc gtttttgacc
     5881 catcttgtct catatatcta taagatgtaa aagaaaaacc tacattttct tttaacatat
     5941 attctatctg aatctctaat tttctactat cccataaatc atcactatct acaaatgcaa
     6001 taaatcgacc ttccgcattc ttaataccaa catttctaga tactgcagct ccagaatttt
     6061 tttcaagctt tatatatttt attctagcat cttcttttcg aaaacttttt ataatttcac
     6121 ttgaattgtc cttagaacaa tcatccacta ataaaagctg ccaattttta taagtttgat
     6181 tttggattga ctttatcgtc tctgataaaa actcttctga attataaaca ggagtaatta
     6241 tcgatactaa aggctcattc atatttttac tctccttata tagcaactag cagtactata
     6301 ccaacaagtt tacttagata ctaattttct ttgtccaata tatttgtaat atcctaatgg
     6361 gaataatgct aaatacaaca atttatcaga tacttttctt aattcaccaa aataatgact
     6421 atcagacaac aatgagtatc tggctaaatt tatgtaattt tttatccaca tatatggata
     6481 tctacttaat ggatatgtat tttgatttat taaatataaa aactctaatg ctctacctct
     6541 taatgcattt ttacttaata tattcttagt caaaccatca tccaaataat ctcttattat
     6601 cataggttta tttacacata taactttata gtatttagac atttcattcc atactacact
     6661 ttctggtaaa aatctcactt cttttctttc tggaaaagga aattttataa gctcatcagt
     6721 tacaaatact atgcttttat ctcccttaac gccatactta aaatataggt ctgcaaagtg
     6781 acatattttc ttatcttcag gcatttctgt ccctatcaaa tctccacttg gatatacaca
     6841 aagtccaaca actccactaa atccttcttt attctctaaa gaatcccact catcattagc
     6901 aatttcaaga gaatctttaa ttaaaccatc atcactatct acaatgaaaa atagctctcc
     6961 atctgcattt tttacaccta gatttattgc agtatgtttt cctccatttt ctttgtatat
     7021 atatcttata tttaatttat tttctgatat aaatttttca actaactctt tagtactgtc
     7081 gctacttcca tcatcaacaa tcaaccattc aaaatcattg aaatcataag tttgagcttt
     7141 taaatcattg tacaaatttt caagtaaatg agctctgtta tacgtcggag taaatacagt
     7201 aaatttatat ttatacatta attaaatctt cctctctgtc taactttttt tgattccgtt
     7261 tattccataa ttaataaagt agtatatagc tttgtataaa ggtatttttt ctatatttct
     7321 atatatattc caagtccaca tagcagcttt tattttatta ccacttaggg aattgctaga
     7381 ttttctatat aatgctagaa cttcatttat tccatgagca atatgccctt ttttcaatat
     7441 cgataaccat aaaacatagt cttcatgtct tacaccactc attcttattt caaaatccaa
     7501 tttagactta tcaatcacaa cagtaagaca tcctaagata tttcctttta ataatctttt
     7561 ataatctaca ttaggtggaa cttttattac tttgtttaat tttttatcat tttcatccat
     7621 cagttcatat gaagtaaatg aaattacata gtcattttct aacataaaat ttacttgttt
     7681 ttctaactta ctgctattcc attggtcatc actatctaaa aaagctataa attgtcctgt
     7741 tgccttactt agtgctaaat ttctagcatt agagacaccc ttattagtct cagtctttat
     7801 acattttatt ctactatcct gttgcatata agatttgact atattaggac tattatctgt
     7861 tgagcaatca tcaataatta acatttccca ttcttgataa gtttggttta atactgattt
     7921 tatggttgct tcaataaatt tttcagaatt atacatggga gtaattatag atactaaatt
     7981 cttcttcata ttaccttcct catcatttat aaaatattta tttaatttta cttaatacac
     8041 aatcatatat cctcttgcag ttcttattat cattatatct gaaaaactta tttctttcat
     8101 ttttttggtt atccataacc tcaaaattat tttcaataat atcaataagt ttattcacgg
     8161 cttcatcatt attataagcc aaagacccaa acatttcact ttttaaatct atatatgaat
     8221 caactttttc catatattca tccaaatcga attggtaaaa caagataggt ctattcatat
     8281 acaaaaagtc tatggctaca cttgaataat cagttatatt tgcagctgat tttctgattt
     8341 catttgctag agttacccct ttatctaaaa acacaattcg ttttcctgtt atattttctt
     8401 ttatatcatc tataaactca tgcataagat gatgcatata aatttttaca tttatatctt
     8461 tctcatctaa tattttattt aatttgtcat catttaaaaa attcattata tttttaaaat
     8521 aatctgtatc cgtaaatttt cctgaactaa attttatcca atctctccat gtaggagtat
     8581 ataatatatc tcgagtttgt gcaacttcat ttgttggtaa attgtcatat cttgcattcc
     8641 caattatata aactgagtca tctaacattc cccaatcatt taccataaca tttctttcaa
     8701 attcagaact tgctgtaact atattatagc ttttatatag gttatctata atatttttga
     8761 actttttgtt cataggtttt ctttttttaa agcttatagt cccatgattt aaaaacactt
     8821 ttatcgtttt tttatgaaaa taattaagaa caggtacagc aaaattgtat ggtgctatat
     8881 ctgctgatgg tgcatgtgaa aatactatag cttttgaatt gtaataatac agatagtttt
     8941 ctacacttcc tcttattaat tttttgcctg gaattttatc aaatacttta ctgtctttat
     9001 ttactaccca ataagtttct acatcattat gttcttttag catatactca taaaaaaact
     9061 ttgaattatc attgtatata tcccctgaat gtccacctat aagccagata tctctaccat
     9121 taaacttatg cttattaaat ggatatataa aaaaagccaa aagcatattt attaaaaatt
     9181 taaccttgtc cataaacacc atcctcatta taacttgtac tgtctaaatc cattaattct
     9241 tccttttata gtggcactat gataatctct attggaactc aatataagac cccctatatt
     9301 tcttacaagt tttgatacca taacttttgc aaacgtataa ttttttctat agactatttc
     9361 ctttttgcaa agcgctccaa agcctcttcc ataattataa tctttttgat acttttcttt
     9421 agatttagaa tgtttttttg caggatggta aattatatca tttccaaagt attttccttt
     9481 aaaacctaaa ctcaataagt ttaaaacata atctatctct tcaccagcac caaactctcc
     9541 accaacaccc aatttttcat caaatcttgt ataatcttta ccttcaaaat caataaaaaa
     9601 agttatggat gttatagtat ccaaaacgtt ggaacttgtg atatcacaag ttccatcata
     9661 cattttctta aatgcatcaa ctttatttga atccatagtt ttgcaactgt atattttata
     9721 atcttcattt ttattaaaga aatttataac tttttctaat gtatcatttt cgtagacaca
     9781 atcatcatca gggaaaccta taatttgccc tacagcatta tctatgccta catttctgtt
     9841 ataactcaat ccttttttag agctttttat atactttata tctaacttat caatgtattt
     9901 atcaactatt tctttaactt tgctattatc attttggtct actactataa gttcaaagtt
     9961 tttgtaagtt tgattttcta agctatccat aagtaagtat atatcatcat atctatttaa
    10021 tgttggcatt attaatgata ttaacattac tacctcctac ttttcatctt ccacaaatct
    10081 atttcccttt tatcatttaa tatcacttct tttattagta atactactgc aaaactaaat
    10141 gaagcttcta ataataaatt ttctgcaaaa gagtatagta aaaatataaa taaagctact
    10201 aaatatgctt ttttatcttt ttctataaag ctttctattc ctttatacat caaatacata
    10261 aataatatac aagcaactac tcctagtaca ctaattatat atacataaga actatctaat
    10321 ggattagttg cttttatagc aggactatat gaatttccaa aaggttttag aaaattcccc
    10381 ttttcagcca agtatacatg ccagaactta ggtctacttg ctaataatct atttaataaa
    10441 gtgcttttat caaatattgt accaataatt acactaaata gagtaagtat tattggtaat
    10501 gtctttagga gcattggcag tccttttatt ttttttatat ctatccatct tattatttct
    10561 acaaatatta atcctgctaa tatagtgaaa aatccagttc tactatatgt ggtttgataa
    10621 acaaaaaatg cagaaccaaa taaaattatt ctatcccatt tattataatc tttatatctt
    10681 aagaatatat atgctgcata tatagtaacc atatgataaa atacactatt tggattacca
    10741 agtccaaaat ccattcttgt aacaagcata tctatttttt ctatatatct atagtggact
    10801 tcctgattaa atattaatga aggaaataaa ttaaataata atatagctac cattaaaaaa
    10861 catgccgata ttattaaata atttttaact ataaatttat catctatttg cttagctgat
    10921 acagctaata aaacgattaa taatatagct ccttccctat ttaataaata aagtaagaca
    10981 caaactccaa ctagtaccat ttctgttata ctttttctct tatcaaataa aaaaagtagc
    11041 attagtattc ctattgaaga ataaaataac gctgatttaa tcatccctat atctatattt
    11101 gggtcaataa atgatatcat atactttcta agtaatatga taaacaaaac aaatatataa
    11161 agatattttc taatcataat atttctatca tcatttaaag tcttcatttt actttctccc
    11221 ttttagcttc gtaaagtatt acttgcaaat attttacaac acttccatat cttatttaca
    11281 cctgttaagt gtttgctttc taagaaataa tttctctttt tagcccaact ttttccccac
    11341 caatgtatcc caaaagtgtt ttctgttata cattctggtg taaagtcttc tttaaatcca
    11401 aaaggataaa aatattcttt gtcgtaaaca cgtattcctt cttcataatt atttgggtca
    11461 atcttcttat aattcttttc cataagatga gtcattattt ttggtattgt aaacaatgaa
    11521 gaagtaagta cttctttttc ataatactcc aataagtctt ttaaaaaagg atgtcctttt
    11581 tctacaccta ttatagcacc atttatatac tctctatcat cttcataccc acagattagc
    11641 ctattatttt ctaaaagagg agttatatcc tttaatattt gcatatctgt atccatataa
    11701 actccacctt gttcatagag tacttttatt cttgtataat cagatatgaa tgcccaaagt
    11761 tttcttttat aacattcttc caaaaactta tttcctttta tttctttctc tatatcaaag
    11821 tttttttcat tccactctac tatctcatat tctggaagct tttctttcca agaatttata
    11881 catatatttt ctatatttcc ttttggacca ccaaaccata cataatgtat ttttttagga
    11941 atcaaatgaa cacctcctac atatattctt gtctatcttc ttcttttaac tgttctacta
    12001 tttttttgac atcttgagct ttatcttttc tacaaaccat aactgcatca ttcgtagata
    12061 ctactatcaa atcactaata cctattgttg atattaattt gtcatctgaa tatataattg
    12121 aatttttagt atctatagta atattttcac ctcttttgat gttaccttca tcatcagttg
    12181 gatatattgc acctagagaa tcccaagaac caacatcatt ccatccaaaa tcaccaggaa
    12241 ctactataac atcattactt ctttccataa taccataatc tatagagatt gattgtatag
    12301 ttgggtatat ttcttttatt ttttccattt cttcttttgt tcctaagtat ttacttatat
    12361 attctaattt ttcatatact tttggcaagt atctcttaaa atcttctaat attttagata
    12421 ccttccatac gaacattcca ctattccata catacttttt agagtttaca tattctttag
    12481 caatttcata gtttggtttt tctacaaact ccactacctc atatgcaaca tcttctatcg
    12541 tattttctct attaaaattt atgtatccat atcctgttga tggaaatgta ggagttatcc
    12601 caatagttac tagtttatca ttattttctg ctatatagat agctttttcc aaaatagatt
    12661 taaattcttt ttcatctttt atataatggt cagctggata tacacacata acaccatcac
    12721 catatttttt cattatgtta aatgctgcaa agcctatagc tgctgctgta tttctggcac
    12781 atggctctgg caatatatta ctatccaaac atttatcttt tactatatcc tttaaagctt
    12841 ccaattgctt ctcattggtt actataaata aatcatcttt ttttgctaga gaatctattc
    12901 tgtttatagt ttcgtttatt aaagcatctt ctccacttaa atttataagc tgtttaggta
    12961 cttcttgtct actaagtggc cagaaacgag ttcctcctcc tccagccatt atgacattgt
    13021 atactttcat tatttatctc cccttagctt tttatataga atccactaca tttaatatat
    13081 aagatattaa attgttcaac ttaaattcta catcttcttg attgtctgaa tttgctccaa
    13141 agtaaaattt aattttaggt tctgtacctg aaggtcttat agctatccaa gagccatctt
    13201 caagtaaaaa tttaagtaca tctgacttag gtaaatcatc aacaccatct ttatagtcta
    13261 aaattttatc tacttttaca tcagccacat tatctatttt aacacttcta aagtaaagca
    13321 tcatctcttt tatttttttt attccatcta taccttttaa agttactgac tttaagtcct
    13381 ctttgtagta tccaaattta ttatatgtat ctatcaaagc ttcatacaag ttcatacctt
    13441 ttgaagaata gtatgctgcc atttcacata tcataagtga agatacaaca ccatctttat
    13501 ctctagcatg agttccaatt aagtaaccat agctttcctc atatcccatt ataaaacttc
    13561 tatcattgct ttcttcaaac gcttttattt tttctcctat aaatttaaat cctgttaaag
    13621 tatttaaaca atctacatta taagcctttg ctatttttgc acctagttct gatgtaacta
    13681 tagtttttat tattgtagga ttgtttttag gaagtttatt ttcttcaact aaactctcta
    13741 atatatatct aactaaaagt gaaccgactt gatttccatt taagactaca tattcaccac
    13801 ttgaatcttt tacaacgata cctactctat cacaatcagg gtctgttcct attatcaaat
    13861 cagtgtcatt attctttgcc atttcaatag ctatattaaa cactgacttt tcttcaggat
    13921 taggatactt tactgttgaa aaattagaat ctggtaattc ttgctcctta actaccatta
    13981 cattttcaaa accacattca tttaagactt ttcttactgg tttatttcct gtaccatgaa
    14041 taggtgtata tattattttt aattttttcc cgtattcatc tattatattt tgtcttaaaa
    14101 cttgcttctt aacagctttt ataaactcat catctacttc attatctaat atagttatta
    14161 aatttttaga taaagcttct tcaaaatcta tacttttaat agtgctgtaa tcatcaatct
    14221 tattaacttc tgctattata tcatttgcca tatctataca gatttgacca ccatctgagt
    14281 catatacttt atatccattg tattctttag gattatgact tgctgttatt actattccca
    14341 tagcacactt taaatatcta acagcaaaag aaagttctgg tgtaggcttt aattcttcaa
    14401 aaatataagc ctttacacca taagcactta aagtctttgc tacttctata caaaactccc
    14461 tagacatgta tctactatca tgtgctataa ctattccctt ttgtttacct tcttctcctt
    14521 gagtcttcat gatataattt aaaacgccca gtgtcgcacg tctaacagta taaatattga
    14581 ttctattagt tcccgctcct attatacctc ttaaaccacc tgttccaaac tctaaattct
    14641 tatagaatct atcttgtatt tctttttcat catcttttat gcttaatagc tcattttttg
    14701 tttgctcatc aaaataagga gaatttaacc acatctcata attattctta taatccatat
    14761 tttcatcctt tctgactcta atttaaaatt tcaaggtaat tatcttcttc taagtagttt
    14821 ttgttttcct tttcttaaaa attcaaaagc taagtccatc tcttctacct taaataaaac
    14881 tatcaataca gtatatacag aagctccacc taatactcca gctccaactg atataatttc
    14941 tttaattgtt cctgaaccca taaatgcata tagattatta tatatcaata aagtagctac
    15001 tgccatcaca ccagatgcta ccaaactttt tagaccagtc tttataattt tatcccctcc
    15061 aaaatatcca tttttctttt taagagatat aaataacaat ataactgtaa ttatattgga
    15121 tgtactagtt gatatagcaa tacctgcatg acctaatggt cttatcaata ttaaattaag
    15181 aactatattt attattaaag ctatacttcc atttatcatt ggcgtttttg tatctgaaag
    15241 agagtaaaat gccctatata agacatctct tactccacaa gccaataaac ctagtgaata
    15301 taatctaagc gctatagatg taagatctgt tgataacgca tcaaatttac ccctttgaaa
    15361 taatatccta acaagaggct ctgctaatat aatagctcct atagatatag gaagcaccac
    15421 caatataata caatttgaag attttacaat agtacttata aatccttctt tatcatcttt
    15481 accagacatt tttgctagct taggatatat aactgttatt atagatgtaa caaataatgc
    15541 cataacaaac tcatttagcc tatttgcata gttaagagca gacaactttc catcacccaa
    15601 agtagttgcc aataatctgt caacaaatat atttaactgt ccaacagcta ctcctaccaa
    15661 cataggcaat actaggttta ctaattcttt tatagactca tcctttaagt ttatatatgg
    15721 cttatacttg taggacttct taaatgcaaa tggcagttga aataatagtt gactcgccat
    15781 agccaatagt gctcctgctg gtagtatcca tacgttttta cctgcactta gtgctattgc
    15841 tgctattatt ataatattat atgggattcc tataagccca ggaattacaa aactgtcatt
    15901 cacctgtaaa aacgaactaa atatcttact tccacttagg aataaaactc ctgaaatcat
    15961 tattttagta aataatattg ctgcttggaa cttagcaggg tcgtttctaa atcctgctgc
    16021 aaatactgta acaaattgct ctgtaaataa aagcccaata atagctatta ctatagccat
    16081 tatatagcat atatttaaaa cattattagt aaattttaag gcaccttcat ctccgagtct
    16141 tttctttgtc tcaaagtaca taggtatata agttgtagca actgcagagc ctatgactgc
    16201 tatgattaaa ccaggtatat ttaatgttaa tacaaatacg tctgcataca tacctgtccc
    16261 atagaaattt gcaagtacaa gttctctaag gaaacctaat acttttgaaa acatagtggc
    16321 tgccataatc cacaatgctg ctttagctgt ttttgacatc ttctttctcc ttatctaatt
    16381 taaactctaa aagtagaact tttaaaactg cttataaaca ttattctaat taagtaaatt
    16441 taacctatga aaagcttcct tataaattaa aaactatttc gactcatatc taagtgccct
    16501 aaatgtaatc aatagtaata atagtattaa tactatacaa gataagaatg ttagattaaa
    16561 tatgctctta aatagactat aatcacctac tgtataccca acttcaacta catcatttat
    16621 attgctatct ttcaagaatt tatcttgtgc acgacttaag ttatcactac aaatcagtat
    16681 tggcatattt tcttttgctg atactactcc caaagaaata tattcgttta catcagatct
    16741 gctaccaaag tctaatttag cagtatagat tttctttata tctttttcat cataaaactt
    16801 ctttattaaa tcaacatttg tacttgactt atttatttgc tttattcctt ctatattttt
    16861 caagttagaa tcaatctctg caataaattc ttttgaatca cctaatgcat atattttttt
    16921 aagattctta ccttttaaga aatcaatttg ttcctcaaac ccttcatctt tagcccatat
    16981 tattggcatt ccacttttag cggcataaga atatacagat atggcattct ctaatcctgt
    17041 tgttgagcta agtaaaaatg cttcatcaaa tcctactgtt ctatacattt ctctagctat
    17101 ctttaaagat gtatcatatc tgtcttttcc tcttattctt tctactttta ttcccatttt
    17161 ttctatatta cgttcagcat ctttagatac tgcctttaaa ccacctacta tcgtaacctt
    17221 ctctggacct agttctttta gataatttct tgttactcta ccaagatttt tactcttagt
    17281 tacaactatt ggagcatttt ttgcatatgc taaaggtgtt gcacttatac tatctacaat
    17341 tgactcttca tttacaagaa ttacttcttt agaatcctta aatgctcttt tatttaattc
    17401 tgtcgaaatc tctggtatac tcccttctat caattctctt ttgggatttg catctgcaaa
    17461 tacctctatt gtgctagaaa tagagattaa caaaactaac gataatatag tagaaatttt
    17521 tttaatcata agttctcatt ccttttcttt tttgtatttt ttgacaataa aaaagctctt
    17581 tttaaagagc tttttcattg attagtaatc taatatttta aaattatcta gctccgtctc
    17641 ctgtcaatac tactttaaca gttttaaaga gtatcttaaa gtcaagtaat atactcctat
    17701 tttttatata gtaaatatct tctttaagtt tttcttcagg agtgatttcg tatccaccat
    17761 ttacttgtgc ccaacctgta agcccaggtt ttatagcgag cctgttgata aatccaggta
    17821 tttcctcatt gaattgaaca gtaaagttcg gtctctctgg tctagggcct attaagccca
    17881 tatccccctt taatatatta aacagctgtg gaatctcatc aattctagtt ttcctaataa
    17941 actttccaac cttagttatt ctaggatcat ctttttcagc ccattgtgcg ccaaattttt
    18001 ctgcgtctgt tctcatagat cttaatttat atatatagaa tcttctcccg cattttccta
    18061 gtctttcttg cttataagtt attggtcctt tgtcctctat tttaataaga attccaaata
    18121 ttgctattaa aggtaaacct atcacaagtc ctattataga taaaactaaa tccataattc
    18181 tttggtacaa gtcgaataaa accgttcctt ttactatgct atagtcaatc tcacttgcaa
    18241 tttctttatc tgcccctgga tagcttttaa ttttaggtat ttcacactca tcattagtat
    18301 atccagacat agttacctcc atattggaat aaaaattttt ttaattctta atttcaactt
    18361 atttctatct tatttttgac ttatttctat attatactca ataatgagca gattatcaat
    18421 acaagaaaca tcattgtaaa caatttgatt ctttacaaaa atatatcatc tcttgttata
    18481 ttttacatta taacaggaga tgatatgttt tgctttaata ttttttattt tattttactg
    18541 tatcttctat tcctattcca atttgagttc ccattgtttt tactttttct gcagtattta
    18601 acttatccat atcgctctta ttgtcaataa atgcacattc aacaagtatt gctggataat
    18661 tgttatttct taacacatat aggtaatctt ttccattatc aagtgttctt gtcttgattc
    18721 ctctattttt catgttaaat ttttctagta ttctttttaa gatatttgat gctgcagtct
    18781 tagttgttcc accattttta tcttttactt tgtaataaat ttccacacca ttaccagctc
    18841 cattagacgc attgtaatgt atacttgtaa ataaatctgg ctttatagtg tttgataatg
    18901 ctgttctttc tcctaaagcc attgttttgt ctgtatctct agtcataaca acatttatac
    18961 cttttgaacg taaatattca gtagttgcta atgctgtatt aagagtatat ttcttttctt
    19021 gtgctccacc atttaatcca cttgttgccc ctgaatcaga acccccatga cctgggtcta
    19081 tcactacagt ttttcctgct gctgaacccg aattatctgg ttctgttggt gcattgtgtt
    19141 ttgataaact agatgctata gaatttataa cactatcttt catcccatca ccaatttgat
    19201 atactgttcc tgctgttttt tctgataagt ttgtgctatg gtatgcagaa acataagtct
    19261 ttggagttat aagtattgga gcttttaatt tagctgctaa aggtcctgct gttattgagt
    19321 caactattat gtcagatttt gcaactaaca ttgcactcaa ctctttatct ggatagaaag
    19381 tcttaattac atttgcattt gtttcatgtc tatctgctcc agaaactcta ttttttgaag
    19441 taccattttt tgctatttta gatatttggt ctataatttt tgatgataaa ctttgactac
    19501 cacctatgta ataagcttct tctaaacctt gtgaagataa ccaattatat gtaccttgtg
    19561 gtacagagtc tttattagct aatattatag gttgttggtc ttcaccagct tttgatgaaa
    19621 tattaagagc atcatattca ccagcatatc catttgctat gtatatttta ttaacatcat
    19681 gatacttatc tatttctttt gctataagta aagatgtttc atgtctatca actccaccaa
    19741 tacgagttac atttacattt acagcagatt taatttgaga tactgccttt tgtgaaactg
    19801 aaccatcatc acctataatt attacatcac ttggatttag acgttttaat tctgcttttg
    19861 tactttctgg tatatcatct ttatttgcaa gtaatattgg tgcatcatat gtagatgcaa
    19921 gtggagtagc tgtaattcca tctgctgcta attctccatt aactattaca accttactag
    19981 acccatcttt ccaaccttgt ttacttatct caacagcagt ttcaaatcta gtagctccag
    20041 caagtttttt tactggcgca gaagtttttc tcatatattc atttactgct tttgttgaat
    20101 ttgcatcact tattataaac tcttgtgtaa catcataatc cttaagattt aatcccatag
    20161 actctaagtc ttctttgttt aaaactttct gttcatcttt accatttatt tttatgcttt
    20221 gaagttccca tacatttaag tattctgggt ctttgttata gtttattaca ccttcatatg
    20281 aatctgtctt ttcattgtaa tttaaagtag cttcaagtgg catatcataa cacataaaat
    20341 ttaatgttat tgaatcagca tttaattttt ctttaaattt tacacttgca ttagatttac
    20401 ttcctaacac tacacttttt ctttgtaaat ttatattaat atctgaatta gattctgtat
    20461 ttgcagtagt attttcagcc gctaaagcta cgtttggtgc caccattgct acagacaaca
    20521 ttcctgttgc caataatttt gttgtttttt tcatcatatc ccccctaatt ttttggttct
    20581 ttaaatcttt aaatcttttt taatacatct taagcttgtt ttatattaca taaattcaat
    20641 attatgtaaa tcttttttca ttatagcaca aaaaattaca aattgtaaac tttttgtaat
    20701 cttttatggc atggttttta aattttatcg aggagaaatt ttgattatat ttgatagttt
    20761 ccgtatattt gaacaaatca aagactttat catctaaaaa tattgcacta aattttaata
    20821 aatatttttt tcttgtacat tatagacatt actataaccc agaataggac atacaataaa
    20881 gaaaatataa gagaatccca agttgtacct gcccaaggtg ttataagttc atatgtagtc
    20941 cactctacca aggtagttgg ataatcgact ttgttcgtaa gttttggaac attccaaaaa
    21001 acatgactca atatctcaag gcacatatat gtaaaaatag ggctacttcc aagtgctatt
    21061 attggggtaa atattttact tttatttttg atgtcacata taaaataaaa tattgacagt
    21121 aacataccat aactgccagc cattaaaagt acaaatgagc tagaccataa tcttttatta
    21181 aatggaaaat actgattaaa gataaaagca cctattagca ggattatgct cataaccagt
    21241 attttaaaga attttttata ttctcctatg ttttctttta aaagtacaca tcccatagtg
    21301 catcccaaca tacctgaaga aattgctact atactagtta aaaaaccatc tggttcaaat
    21361 tctggcttgt acaaatgtcc cttgaagaaa tgtaaatcca ctaactgtgc aagagaacct
    21421 tctagctcaa atccataagg ttttgctaat atgtagtacc caactattat aaatgtagct
    21481 atacatagaa aagtaattat tgtagcagta cttccaacat ttaatttttt tagtagtaaa
    21541 tatactaaac tagtcacaaa atagactaat cccatacgtt gaagtactcc aagtattctc
    21601 acagaattta aatcaggatt tcctaggtaa tttaagaaaa atccaaataa tatcaataag
    21661 atacttctct tgaatatact aagtattata ctcagtgtgg atttattatt ttttagttta
    21721 ctatttattg atattggaat tgtaacacct aaagatataa caaaaaaagg aaatgcaaaa
    21781 tccgcaagtg ttacaccatg ccatacagca tgtcttaatt gaggatacat tctcatccaa
    21841 gtcccaggat tattgcagac tatcatgagg gctattgata gccctcttat tatatctatt
    21901 gatttaatcc tactattcgt aagtttagaa tctacaacat tatttgaaat t
//
LOCUS       DAMKPG010000065        21834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMKPG010000065 DAMKPG010000000
VERSION     DAMKPG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.068"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            48..923
                     /locus_tag="PC621_002498"
     CDS             48..923
                     /locus_tag="PC621_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF4109645.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIKEYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1284..2582
                     /locus_tag="PC621_002499"
     CDS             1284..2582
                     /locus_tag="PC621_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF4109646.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            2772..4163
                     /locus_tag="PC621_002500"
     CDS             2772..4163
                     /locus_tag="PC621_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF4109647.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4340..4927
                     /locus_tag="PC621_002501"
     CDS             4340..4927
                     /locus_tag="PC621_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4109648.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4960..5751
                     /locus_tag="PC621_002502"
     CDS             4960..5751
                     /locus_tag="PC621_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4109649.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5932..8349)
                     /locus_tag="PC621_002503"
     CDS             complement(5932..8349)
                     /locus_tag="PC621_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109650.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(8362..9027)
                     /locus_tag="PC621_002504"
     CDS             complement(8362..9027)
                     /locus_tag="PC621_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109651.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9109..10140)
                     /locus_tag="PC621_002505"
     CDS             complement(9109..10140)
                     /locus_tag="PC621_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109652.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10130..10816)
                     /locus_tag="PC621_002506"
     CDS             complement(10130..10816)
                     /locus_tag="PC621_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109653.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10974..12038)
                     /locus_tag="PC621_002507"
     CDS             complement(10974..12038)
                     /locus_tag="PC621_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF4109654.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            12300..12971
                     /locus_tag="PC621_002508"
     CDS             12300..12971
                     /locus_tag="PC621_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109655.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            12973..13881
                     /locus_tag="PC621_002509"
     CDS             12973..13881
                     /locus_tag="PC621_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109656.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            13958..14644
                     /locus_tag="PC621_002510"
     CDS             13958..14644
                     /locus_tag="PC621_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109657.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            14641..17196
                     /locus_tag="PC621_002511"
     CDS             14641..17196
                     /locus_tag="PC621_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4109658.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
     gene            complement(17390..18589)
                     /locus_tag="PC621_002512"
     CDS             complement(17390..18589)
                     /locus_tag="PC621_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF4109659.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            18708..19595
                     /locus_tag="PC621_002513"
     CDS             18708..19595
                     /locus_tag="PC621_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4109660.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            19785..20342
                     /locus_tag="PC621_002514"
     CDS             19785..20342
                     /locus_tag="PC621_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4109661.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(20582..21442)
                     /locus_tag="PC621_002515"
     CDS             complement(20582..21442)
                     /locus_tag="PC621_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF4109662.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         8168 a         2712 c         3170 g         7784 t
ORIGIN      
        1 tattaaattt attttattga tgaaactaat ggggaggtta aaactcaatg attacttttg
       61 atgtaaatgg agctattatc agatttgatg atacaaaaaa taattataat aagataagga
      121 aaatctttaa attatatgct ttagaaatat ctaaaaactt tgagagagat tgtttaaata
      181 gttttcaaaa tctaaagcaa atatctgata gaggtttaag tttaggagaa aaatatatag
      241 aagagtcttt taagaaagga attgagacta tagtaagttt tggaattata actatagata
      301 tcgatacctt taaaagcgtt tattgtgaaa aatatttaga ttttaagaga ctttttaata
      361 acttgaataa aacttttatt ataccaaata aaaataaaaa aaatgggcac gctagcttct
      421 tagaaataga atcgacaata aagaaaatga gcaattattt atacaatgat tgttttaaaa
      481 ttcattatgc tgttgtagat gcccttatag aaaatcgtat aactgcggta cattcttata
      541 ttgatgaaga atctataaaa aaatctaatg ccttatttaa taattataaa gatggtttta
      601 ttgggaaggc tgacgaatgc aaagtagtaa aacaaataat aactttaaat ccatataggg
      661 aagatgtata cgaatttttg gtaaaagaag atggagattt tagtaaggaa attgagagat
      721 tgactgaatt tttaggctat gatataaaag aatataaagc gtcattaatg gatatttata
      781 taaatgagtt aatggagagc gatgaaaatg atgttgaaaa tgcaaaagaa aagattgaaa
      841 aatatgctag atatattgga tgtagtgatg agaatttata tgtagcaaga gttaatgcca
      901 tatatacttt tgcaaatgca taattttatt aaacattaaa tattagatgt tcaattatat
      961 aaatttaaat aaaaaaatat ccttattaat aacaaatagt ttatattaaa aaccattctt
     1021 aaaatgagtg gttttttatt ttgaatagat atattttaaa cctaatagca ttcctatctt
     1081 taattagcca aaaatcaact ggatttcata tagactacat aattttaaat tactaaaaaa
     1141 gacaatatat tgacaaaaat gaaatatgtt gataaaatga aatagtcaat atggtgtctt
     1201 aatttaaaaa tttatttatg ttgtattaga taaagttgtt ttatatataa agcataaata
     1261 attattataa ggagaaatag attatgaaag taaatgtata caatgtagaa tcatttgtgg
     1321 atgaaagact taagattatg gtttcagact taattccaaa tactaaactt aaaataagtg
     1381 tgaaaatgga atttccatgg tgcaagggag aagaattttc atcttatgga gtgttttgtt
     1441 caaatgaaaa aggagaagta gatttagatt tagttgaacc tatagaaggt acttataagg
     1501 ctgataattg tatggggtta atttattcgc tggaaaaatc taaaactgaa ggtaaaaatt
     1561 ttgcagaaaa tatatctatt gataaaccaa taattatgaa tatgatattt gaaacttcta
     1621 ctgaaagaaa agaagttgaa ttaaagagaa tatttaaatc agaagatatt accacaaaat
     1681 acatatctga tgagtttata ggaaaattat tttataaaca aaattctaat aataaaacaa
     1741 ttttaatgct aggtggttct gatggaaatt tagacgctct agacctttta gcagctccac
     1801 ttgcatctag aggattcaat gtactaacag ttgcatactt tgctttagaa gggttaccag
     1861 ataaattaga agaggttcca ttagaatatt ttgaaaaagt atttgagtgg attagtgaaa
     1921 atgaaattac cagtactaaa gaaatatttg tacatgggac atcaaagggt ggagaattgg
     1981 cctttttact tgcttcaaga tatgaacaaa ttaaaaaggt tgtagtatca caacctcaca
     2041 tttattgttt ccaagctctt aatggattga tgagtggaaa tgatacttca tcatggtcat
     2101 ataaaggaga gccatttcca tatatcaaag tagacaatga tatattcttt gaagaacaga
     2161 aaaaaaatat atcaaaaggt attccatttg gatttaatag tacttataag aaaagtctta
     2221 aaagagcaga gaatagaaaa gaggctagaa taaaaataga aaattccaaa gcagatatac
     2281 ttatgattgc tggaaaagaa gataacatat ggaatagtta tgaagcttgt ttagaggcat
     2341 tagaaatctt tgaagaaaat aattatccat ataacgtaga attattaaca tacaataata
     2401 tgggtcatcc gttaccaatt ccatatatta tgcctctaaa ggagacttta tgtatgagta
     2461 tgagtggagg gattttttct tcaggtggaa ctgtagaagg aaattcaaaa ggacagtttg
     2521 aatcatggaa tagaactatt gaattttata aaaaactttt atcaagtgat ttaaataact
     2581 aatattaagt gatttaagta actaaaaatg aagcggtttt tctatatatg cacaaaatct
     2641 atcaatttta tagatttaat ttattgttta tataatttgc atgttatata ctaattttaa
     2701 tgacattgac aatttataaa caattataaa gttgtaaact tataagagtt attgaaaggt
     2761 ggagtgaagt tatggaaaga ctcgctagaa gggaacaacc tcatataaaa tttggaatgt
     2821 ttaagataag aattccattt atacattaca gatttgagaa accagaagct attcaaggta
     2881 taatatcaag tatgacaagt ttaggcacaa ttggccttag tacacaaatt ttgggattac
     2941 cctatgaagt ggcatggagt atggctatta ttaactcaat attatactgt ttgcatgttt
     3001 ttatgggaga cccagttgta cctggctgga ttacagcttc tataactctt acaactgcat
     3061 atttgttaaa gttcagcatg ggcattgaaa gaatacaagc attaacagct ttacaaatag
     3121 atttaggaat aatatttatt ttaatgggta taactggtgt agctggaaag ttagttagta
     3181 agataccaaa ttctattaaa ggtggaatac taatgggtgt cggtatatcg actataatat
     3241 cagagtttaa tcctaaaggg agatttgatt cttatcctat atctataaca gtaggaattt
     3301 tggttgcttg ttttgtaatg ttttctgaaa gatttgatac tctaaaagtt aagaataaat
     3361 ttttatttca tttaggagaa tatggtgtag tatcagctat tttagtgagt ttaatagtag
     3421 gtatatttag taaagaaata gaaattccta gatttagttt tgacaattta gtttacatag
     3481 tagattttaa aaatctgata aatacagtat ctccttttgg aataggtttt ccaagtgtaa
     3541 tgttatttat acaagggata ccaatggctt ttatgatata cataattgcc tttggtgatt
     3601 ttataacagg tgaaaactta gtactttctg aaagtgaaaa tagaaaagat gaatatatag
     3661 atttcaattc aaataggtct aatgttataa gtgggataag aaatattttt atggcaatct
     3721 tttctccata tataccaatg tgcggtccat tagctgcgac acttacagga agtgttgctc
     3781 aaaggtacaa ggttggaaag gaagccatgc agtctgtttt tagtggcatg ggtacattag
     3841 tatgggtttc agcaattttt atctgttttt atccaattgc gcagattgct acacctctta
     3901 taccattagc attgtcagta acacttttag ttcaaggata tttatgtaca aagctatcaa
     3961 tggagctatg taatacaggt gttgagaagg gattgattgg acttatggga ggtgtaattg
     4021 ctgctaaagg cggaacatgg ggacttgctg taggttttat actgtatttc attttaattg
     4081 attctaaaaa aagaaaagaa aaagatatta attcaataga agattgtgta tatgaaatat
     4141 tagacaaaaa gaagagtatt taaaggaaaa tatgagttgc tgttatctat taatagatta
     4201 attgcaactc atgttttatt tataagtttt aggaattgca aaaaaatgca aagctttttc
     4261 ttgacataat ttcttgatat gttactatag ttatataaca tatgtgtttt ataaaaattt
     4321 aatggagagg tgaacatata tgccacctaa agtaaagata acaaaagaaa tgattttgaa
     4381 tacagtcttg gatattacaa gagaagtggg atttgaagct gtaaatgcga ggagtattgc
     4441 aagtaaatta cagtgttcca ctcgaccaat ttttacttgt tacgaaaaca tgaatgaatt
     4501 aaaaaatgaa tttcttgctt ttgcatatga atattataat cagtatgttg ttaattatcg
     4561 taattctaaa agtgtaagcc cttatttact gcttccgctt tcatacattg aatttgctca
     4621 agaagaaacg tatttattta agttgttatt tataaatgat ttagatttga tgatgaaaga
     4681 ggcgaatgat ttttataaag aaattgacaa tgaaaaaaag gcaagacttt tttcggaaag
     4741 cattggaata gagttggaat gtgcaaaagt aatattttta gatttatttc tttatactca
     4801 tggcatagct gtcttaacag caacaaaaaa attaacatta gatagatata gtgctgaaaa
     4861 aatgttgaaa aatatattaa cagcttttat aagacaagaa aaaccagatt ggaatttgtc
     4921 tatttgatgt ttattaacta atatagaaga ggtgagatta tggaaacaaa acaatactta
     4981 attgactttt ataatactta tgatgaagat agtcgacttg cattaaaaca tggaatggtg
     5041 gaatttctca ctactatgca ttacattgat aagtacataa aatcaggtga ttgtgtgctt
     5101 gaaattggcg ctgcaactgg tcgttattct cacacactgg cacgtcaggg atatgatgtt
     5161 gacgcagtgg aattagtcga acataatata gaggttttcc ataaaaatac tcaatcaaat
     5221 gaaaatataa gcattactca gggtaatgct atggatttat ctgttttccc agataataaa
     5281 tatgatatta cgttactgtt aggaccacta tatcatcttt ataacaaaga agataaacaa
     5341 caagctttac atgaggcaat tcgtgtaaca aaacctggtg gtgtggtttt tgctgcatac
     5401 gttatatctg atggctgtct cattgacgag gggttccata gaggaaatat taatgtatct
     5461 gaatatattg aaaaaggttt gattgatcca cagacatttg ctgctaagtc tgaaccaaag
     5521 gatttgtttg agcttgtacg taaagaaaac attgatgatt tgatgtctgc atttaatgta
     5581 actagactgc attatgtcgc atcagatggt ttggctttgt atatgcgtga agcagtagac
     5641 tctatggatg atgatgcatt tgcgttatat ttaaaatatc accttgctac ttgtgaacgt
     5701 gaagatttag taggagttac aagtcatgca attgacatat ttagaaaata aggaatatgc
     5761 ttgtttatgt ctctggtgat gttgcttgga gatataagga agtgtatctg tataaaataa
     5821 aaacaagtag agggacagaa aattatgtaa agtcaaaatt ttctatcctt ttagctttta
     5881 aaaaattatg ttaaaacttg tataaaaaat ggttattttt tatatacatt cttactgaga
     5941 acgtaagcgt tctgtaagtg attttttttc tatatttctt ataagtaatt cagtcactaa
     6001 aacttgtata ccaattaata cgaatgaaaa aattaaaata gtaaatatag aaaatttaaa
     6061 attaataaag tctaatctaa ttacatcttt tataaaagtg caaagtagat ttgaaccaaa
     6121 ataacctagt atacttgaag tacatatagc tttgatactt atgtttagac cttctctata
     6181 aagcatattt cgcagttgct ttcttgtcat tccaacggct tgaagtaagg caaactcttt
     6241 ttttctaatt aaaacgctgt ttattagggt attggtcata tttagtatac caaagcaaga
     6301 aattaaacca acaaaaatgt atgaagctat aattatatat tgaaatgctc tagtaatcat
     6361 tctataatca tcttcatatg aataaagcat taaatttgaa tttttctgaa ttatacttaa
     6421 caacttttct tcaacttctt tagaatgctt tttatctatt aaaatagaaa aatctaaatt
     6481 tgtattatat ggtgttagag attctaaagt tttgtctgaa gcaaaaaaca tacctcctgt
     6541 attggaattg tcaatgattc ctataatttt cattttcttt ttaataatat catttccact
     6601 aaagatatta aatgtaactg tatctccaac ttttaaattt aatccataag attttgttct
     6661 atatttattt ataattaaac tatctgaatc taaatgtgta tagtctattt ttccactgac
     6721 aatatattct tcaagtgatt ttgaaagttc aggcgtaata cctttaatta gagtaataaa
     6781 ttgtttttcg tctctatctt tgatgtaagg ttctataata ttacttttaa ttgatttgga
     6841 ttcaattaat ttttttactc catgaataga tttaatttca ttcttaagag aatctgttag
     6901 tggattattt tgttggattt ctgtaatatg ctcatataag ttatcactct taatgctaat
     6961 tatataatct tcgttataga caaattgttg agatagttgt tttttgaaat ctattgaatt
     7021 agcaagatta gcgattgaaa taaataaaac tccactaatg cttagcgata ttaatgcaat
     7081 caaatttttc tttctatttg actcgatatt ctttaatgct agatttttta tatttatata
     7141 actaaaattt tttgcttgtt ttctaaattt agtttctgta tattgcatgg cttcaatagc
     7201 agatatttta gaaataattc tagctggttt aaataaagaa attctaacag ttaaaaacat
     7261 taccagtgat aaaattaaaa ttataaataa atcagctatc atttgccatg ctaatggttg
     7321 aattatgtaa gcaattatac aagaagctat agcccctaat ggaataaaaa taattgacaa
     7381 aatatttcct tgtagagaaa tagtcttttt taactgaaaa gaagtcatac ctaacgattt
     7441 caacttagca tacatctgaa ctgaattaac aactgaaata taaaaaatac tatatataac
     7501 aaaaacgcta gaaaaaagaa caactattcc aactactaaa actgtaataa tctgctcacc
     7561 atcaattaaa tttgattcaa tatagttatc atttaagtat atattatatt tcttaatacc
     7621 taaagataaa ccaattttct ttaactgtgt cttaattgaa gctgaatctt tctctgtatt
     7681 ttcttctgat agtttaacta atacatctac agtatcaggt cttgactcgt caaaagtatt
     7741 acttttattt tgtaaaggtg tagaagctat attcttaaaa taatcatttg aaacgactgt
     7801 atagtattgc ttggcattat cttgataata attttctaga aatccaacaa taataaaatc
     7861 acattgaact tctttattcg taatatcatc ataataattt aatttaatgc tatctccaat
     7921 atctttatct aaaccaaatt cctgtaaata tttttctgaa atagcaattt catttttatt
     7981 tgtaggtagt ttgcctttca atggttgaaa attcataagt ttaattgctg tatcatctgc
     8041 ataaacaatc gacataatac agtctgtctt tttattactt ccaattaatt tgtaggttcc
     8101 tacgccacta aggtttttat ctttatatag tatatttttt gctttttcat ctacagattt
     8161 atatatagca tgatatggag atgcattaac taaatctaca gaagcattat atgaaactaa
     8221 ggtcactgtt aataataaac atgtagaaat cattataatg atacctgaaa gatatgtttt
     8281 aagtttatta atttttaaat catttaaagc tatttttctt ataaatgtgc gattattatt
     8341 tctaaacata tactacacct cctagttaac aatttttcca tcttcaatac gaataattct
     8401 atcagttagt tgtgcaattt cattattgtg tgtaatcatt acaattgttt ggttgaattt
     8461 tttacttgtc actttaagta aactcagtac atctgaacta gttctactat caaggctacc
     8521 tgtgggttca tcagctagta taatagctgg ttttgttaca agtgctctag ctatagcaac
     8581 acgttgctgt tgaccaccag aaagattact tggcatatca tataattttt cttcaagaga
     8641 aagcatattt ataatttcat ttataaaatc cttatctatg gtctctccat ccaatcctat
     8701 tggtaagata atattttcat atacattaag taaaggaaca agattataat tttgaaatat
     8761 aaaacctata tttctgcgtc taaagattgt aagctgttca tcagtcatag tagataattc
     8821 cttacccttt ataataacct ttccagatgt aggattatct aatcctccca tcatatttaa
     8881 taatgtggat ttgccactac cagatgtacc tacaatagaa ataaattcgc ctttttcaat
     8941 agatagagtt acaccatcaa gagcttttgt aatgtttgaa ctttgtccat aatatttctt
     9001 taaattaatt acttgtaaaa tactcataat aatacctcct taacatcttg taaatacagt
     9061 ataaaatcaa aatgttgcag aatcattaca aataaaatat ttaaaaattt atttattagg
     9121 taagaagata gaaaaagtcg acccttttcc aatatctgat gtaactttaa tatagccacc
     9181 ttgaagagat ataatttttc ttgaaagata tagacctatt ccaatacctt ctatgtcatg
     9241 tacatcttct tcacgataaa atcttttaaa gatttctgct tgatgacttt ctgatattcc
     9301 ttttccatta tcaataatat ctatttttgt atacataacc caacattctg tagctacacg
     9361 tatcttacca ttagaatttg tgtattttac agcattatct aatatattaa aaatagcttc
     9421 agaagtccat ttcttgtcat gaattatata taaatttggg tcacaatcaa ctgtaacttc
     9481 aatattcttt ttttcagcat caaacaaaat tccactaagt gctgtagcaa ttgtttcata
     9541 aattgaattt ttctttttag atagtgtgat tatacctgtc tctagtctgg aggtttttac
     9601 catagattgc attaaaaaat ctaatttatc taattggttt tccatatcca ttaaaaaatc
     9661 cttttgaatt tctgttttaa atgtttgatt tagtagagta gtattaatca tttttaaatt
     9721 tgcaatagga gttttaattt gatgagatag atcagaaact agttcttgta aatcagcttt
     9781 ctctatagca atactattgc gattttcttg aataacttca tacattctaa ttaatctgtg
     9841 attgatacgt gataaaatag tttcttcttc taattcaatt tcaatattgt cttcactatt
     9901 catcatacta tctaaagtgt gacatatttt agaacaaaat ataactattt ttttatgaag
     9961 taaaaatatt attaatgcta taaatccaca aagtactatt gtatatatta ataatagagc
    10021 aattagtttt aaatcatcag ttattagata aattgaaata ttaaataaaa tagctgtaaa
    10081 aattatacca acagcaatta ttaaataaat agaactaaaa gaaaattttt taaaattcat
    10141 ttctttcacc taaccacata tatcccatac catataccgt tttgatatac ttaagattgt
    10201 ctttttcaat tttgcttcta atcctactta taacagatgt taaagtatgt tcatctacaa
    10261 agttagcatc tatatcccag agtttttcta aaagtacttt ccttgttaaa atacttttag
    10321 gattttcagt aagcagtttt aaagtacgat attctaatgg tgtaaatata atttgatttc
    10381 catttatatt ggcagataac tcagaaaagt taatttcaag atatccatcc ctatagtaat
    10441 cttgttttgt ttttgttgta agatgattta aaaaagcttt aactttctgt ttaaataaat
    10501 tgatattaaa tggttttgtt atataatcca aagctccaag ttcatagcct ttaatcatat
    10561 cacattccat atcattagca gttaaaaaaa ttattggtat attgtaattt tcctttattt
    10621 cattacataa ttcaaaccca cttttatctg gtaaattaat atccaaaatt attaaatcaa
    10681 attcacattc aaaaatagac tttagtgcag atttagctgt aaattttgaa gtaattatat
    10741 atccatcttc aattaggtta tatgatagag ttttatttaa tatagaatca tcttctacta
    10801 ctaatatttt ttccatacag ttactccttt tagtttaata ttttataatg tcaattatag
    10861 actaaaaaca ttgctgaatt gttataaacg agaaaaagta tttaaaaatt tatatattta
    10921 ataaagaacc tgtcctatga taaattctag tacagattct ttattaaata aaattatttc
    10981 aaccaagatg gatttccagg tataaatatt cttttaccaa attttgaagt atactcatct
    11041 aaagcttttt gaataagctc attattaccc ttaaatgaag tagtattctt tatagtttcg
    11101 agattttggc taaatgggca agcagatata cacataccac aatctgtacc taaatatctc
    11161 catttaatat agcatgtctc atgttcaatt acccaattat attttccatc atcaccaatt
    11221 ttaatatcat tagaaagcga atgagaaggg cagttaaaag agcatttttt acagacctta
    11281 caaaaatctt ctagtccaaa atcaacatat tcatcttcta aaagaggaat atctgtagta
    11341 acaacaccta atctaagtct agagccataa tctttatttg taagtatagc atttcttcct
    11401 atatctccta aacctgcatc acgagctata aaaacaggca ttactaaata attagcatcc
    11461 atatgatttc tagcatcata tcctaaattt cttatatagt aacttaaaat catgccgacg
    11521 atagatgcat ccacatagca ctttgatgtt tctataactt cacatatcat tggagctcta
    11581 tttatcattt ctttatccat ctcacatcca aatacaattg catacttatg agttagatta
    11641 atttcttcac cgtaattttc ttcatgtcta cccctatttg tataaatatg atagtctttt
    11701 aatttagtta taccaactac agaagctcca tactgctttg caagaccctt aatttttttt
    11761 gtcattgact ttgcatctac atctacttta gtatcagaaa cttttccttc gcataaagat
    11821 ttgatatcag ataaaaaatc gaatgcacta cttgccattg gagagtttaa ctcattgtaa
    11881 gtcattgttc cttcactaca taaatttggt ctatttctta tactatcatc aatttctttt
    11941 ttgtcaggat ttttcttata ataatcatta taagcagtac tatctttttt gtaatttgct
    12001 cttgcaaaca tagtatttct ctcatcaacc cttttcaatt ctattccccc tagtaaaaat
    12061 atatttaaaa aatataactt tattgttgta taatttaatt atataagaat aacgttatat
    12121 tttcaattta agcgtaatta aattgggaaa tatataacta tatgcttgat tatgatgttt
    12181 ttattttgaa aacaaattta aatacaatct ttttagtgaa ataaataaaa ttaaaatatt
    12241 tttagtatga taatgcaaga aaataattat ttatctaaaa aatctaaagg agagtaaaat
    12301 tgaacttatt aattattgaa gatgatataa acttaaatga aggtcttttt tatgcatttg
    12361 agaatgatgg atttaatgta tttaaggctt atacaaaaca agaaggatta aatatattta
    12421 atagtaaaaa tatagatttt ataatcttag actgcaatct tccagatgga gatggatttg
    12481 atgtatgtca aataataagg gaaaagtcag atataccaat tataatgctt actgcaagag
    12541 actcagaaat tgatgaagta aaaggattag aaatagggct tgatgattat attacaaaac
    12601 cattttcttt atctgtatta aaggcaagag tcaaagtagc aataagaaaa aaatcgaata
    12661 ataaagtaat atattccaat ggaataaaat tggaccaaaa gttactaaaa gtatataaaa
    12721 ataaagcatg tttggaactt agctctgtag aatataagct tctaagttat ctaattgaaa
    12781 ataaaggtca gatattatta aaggaacaaa ttttacacca tatttgggac agcgaagaaa
    12841 attatgttga tgataatatt gtatctgtaa atataagaag acttagagta aaagtagagg
    12901 atgacccatc taatccaaaa tatataaaaa cagcttatgg aatgggttat ttatggaatg
    12961 aggtagaata atatgtttat attagccttt ataatatgta cagttgttat atttatagta
    13021 tctactaaaa taaatcaaag aagatatgat gaattaaatt tgatgttaaa tcaaatattg
    13081 gaaggtaaag aagttactta tccagatacg aaggatacaa gagcgtcaaa aatttctcac
    13141 caagtaaaaa aaattaagga tatgatagaa atagaggtag aacagtcaaa gtcagaaaaa
    13201 gaagcgatta aaggtttaat atcaaatatg tcacatcaat taaaaactcc actttcaaat
    13261 ataaccattt attgtgaact tctggagaat ataaatattt ccacattaca gaagaaggag
    13321 tttttacaaa agatgaaaaa tgaaactttt aagattgact ggttgttaca atccttattt
    13381 aaaatgacta aattagagga tggagttata gaatttgaag tagaagaact actaattaaa
    13441 gatactctta ttcaaagcat cagtactatc tttaataaat cagaagctaa aaatataagg
    13501 ataaatctcg aaccattttc agatataaag ttagttcata ataaaaagtg gaccatagaa
    13561 gcaattgtaa atgttttaga aaatgcaata aaatactcac catcagatag cacgattact
    13621 atttctgtaa taaaaatgga gctatatacg aagattacta ttaaagatga aggaataggt
    13681 attgatagta gagaactaaa tgatattttt aaaagatttt acagaagtaa aaatgtggaa
    13741 aaccaaaatg gcacagggat tggtctatat cttacaagat taattttaga aaaagaaaat
    13801 ggaaacatta tagtagaatc taaactagga agtggatgtt gttttagtat attcttacag
    13861 aattgtaaga gtttaaacta actttgtagg atgagtgtaa ttatcgtcac ttacaatatt
    13921 ttatagaaca tatatctgta ttgaaaggag aaattaaatg agtatattag tgacgaataa
    13981 tttagtgaaa cactatggaa aaggtgagac taaagtaaaa gccttaaatg gagtaagtat
    14041 agaaattgaa cgagatacat ttactgctat agttggaact tctggttctg gaaaaagcac
    14101 attgttaaat attataggtg gattagataa tccaacctca ggtgatgtaa taattaaagg
    14161 aaagaacata tctaaaatag gaaaaaaaga tttgacagta tttagaagaa gaaatatagg
    14221 atttatattt caaaactaca gtttaatgcc cgtattaaac acttatgata atatagctct
    14281 tcctgttaca ttagataaag gaaaccatat agaccatgaa tatattgaaa tgctgatgaa
    14341 gacattggga atttgggata agcgtttaaa atttccaagc gaactatctg gtggtcaaca
    14401 acagagggta gcaattgcta gagctttggc aaataaacct gcacttatat tagcagatga
    14461 accaacagga aatctcgata gtaaaactac aatggaagtg gtatgtttat taaaagaaag
    14521 tagtgcaaaa ttccatcaaa cgatacttat ggtaacacac aatgaaaata tagcacaagt
    14581 atgcgactca attatccata ttgaagatgg agttgtggtg aatactggtg gtgaagtact
    14641 atgatgtctc tagtaaaatt ttcaattcaa tatataaagc attataaaaa acaatctata
    14701 tctatagttt taagcatagt actatcagta gcattattga cgggaattgg ttctttagtt
    14761 catagtgcag acaaaagtag aattgaaaag ataagggaag atagtggaga ttatcattat
    14821 ttctataaag tagataagga acaacttaat aaaattaaaa aaaataaaaa atcaaaggaa
    14881 tacattgtag atagacttgg cgttacaaaa cctaaaaatc agatagatga accttttatt
    14941 atagaatttt taaatgctga ttcttcatat ttagacatga caggtagaaa attattaaaa
    15001 ggaactcttc ctagtaaaga tggagagata gtacttgaca catattctct aaacaattta
    15061 aatgtaaaca aagaaattgg aacagaaatc aatttagatg gtaaaatcta taagttatgt
    15121 ggtattttat caagtctttc aaacccagaa actgctatgg agggatttgt aaacgattat
    15181 ttgcagccta aagaaaagga ttcttatata gtttatgtaa aatatgatga aaataaaaat
    15241 attaaaaagc aaaattataa atttatgaaa aaattcaaca tatcaaaaga aaaaacttat
    15301 ataaactggc ctctttcagt tgaatttgga gtaaaaccac caaagaatat agaaaaatat
    15361 aatatatttg agttcttaaa aaatttagaa ctaaatacaa atgcaattat attgcttgtt
    15421 ggagtgtttg gtgcagcagc agtatatagt attttccatg tttcaatact tcagagaata
    15481 tcacaatatg gggttttaga agtattaggc gctaataata aacaactttt atttctacta
    15541 tttttagagt tatttttact ttttattgtt ggatttccta taggttgctt cttaggtatt
    15601 ggagtagcgt ctacaatata tgaacaattt ccacatatat ttttgagtag tgatattgtt
    15661 ccaggagcat tttttataag tgaaaaatct atttatttag gctttttatt tatcttattg
    15721 ttgttaattt taattgctgt acgtgttgta catcagttga ataaatactc tagtatagaa
    15781 tctatgaaaa actttaagat tactaataag aaaaagagaa tattagaaag tgtaaaacac
    15841 tctaacatga caaaagtatt atctcataaa tacatgactc aaaaaagggg aatgtttatt
    15901 ggaattttgt tttcattatc acttggtgga attatatttc tatgttctag ttactctata
    15961 cagcttacta aaaacaataa tgaacttaca atgaaagctg atgatggttt aaattctgac
    16021 taccaaataa ctatgcaaac aacagatttt gatgtaggta taccaaaaga taaaatttca
    16081 agattaaaca atgttgaggg aatatcaaaa gtctatccag taagatattt atttggagct
    16141 atgcaaatta aagaagaaca actattttgg aaaaatttct ttaaacctct tgaaaaagat
    16201 tacaggataa agactttttt tgatggtatt tgtacaaagc aaaatgaagg tggctatctt
    16261 ttaaaaacaa atatttatgg ttataacaat ggtatgttag aaggattaaa ttcttatatt
    16321 atagatgggg agattaatac ccatgatatg gttaaaaata ataaggttat agttagacta
    16381 cctatggatg gaactgggat gtatgatgca gttgatataa atccaggaga tacaataaaa
    16441 gttaaagtgc caaagacaat gaaaccaaca ggtgaaattg ttaaatttaa agaagaaaat
    16501 aagaatgatt atacaacaaa agaatttgta gttgctgcta ctgtaaaacg tgtgatggca
    16561 aataacatat atttcattgg tgattatggt atggatatag ttatgacgaa tgggcaaatg
    16621 aaaaataact ttgatatcga aaattataat tcagtaagta taaaaaagac agaagatagt
    16681 aacagtgagg aattgtctga gaacataaaa agtgttgtaa gtgatgtaaa aagatgtatc
    16741 gtaacagatt ataccattgc tattgaaaaa aataatacct acttaaatca aaaactctta
    16801 tttatatatg gtattgtatt tgtacttctt gcaataagtt tgttccatat aatcaataca
    16861 gtaagctact taattttttc aagaagacat gagtttggaa ttttaagagc tatgggtatt
    16921 acggataata agtttttact tatgatgata agagaaggtt ttttatatgg attctatgca
    16981 agtattataa tggtaattgg aagtgttatt ggacaattta tgatttattt tatggtaaaa
    17041 cgtgtatatt tatatataaa tccaattctt aaaataaata cacctctata cataggtatg
    17101 attattttaa atatcactat tagtatagtt gctgtaatta ttccagtaag acaaattttg
    17161 aagagtgata ttatttcaga aataaataag agttagagtt atataaattt ttaagattat
    17221 ataactttat gacaatcttg tttaaattat ttaaatatat cttatctgtg aacattaaat
    17281 aaacctctta atagatataa ctcttgaaaa gtcatatcta ttaagaggtt ttaataaatt
    17341 ttcatgttaa tatatataat ccaaaaaaat taaatcttat taaaacatac tattttgtat
    17401 agttatcgaa atatccttgt atgtaaataa taggagtacc cttatctcca cttccagatg
    17461 ttaaatctgc taaagaacct ataaggtctg ttaatcttct tggagtagta ccttgagaaa
    17521 ccatgtttcc tgttaaatca tcagatttat tatctttttc aactatgtat tttgaaattg
    17581 cttcttttaa ctcatcacca gataagtcag caaaatcatt atctgctaag tattttaatt
    17641 ttacttcgtt tggagttccc tctagccctt ttgtataagc aggagatact actgggtcag
    17701 ctaattccca tattttacct acagggtctt taaaagctcc atctccataa accattactt
    17761 ctacatcttt ccctgttatt tcttttatat taccttggat tttgtttaca acttcatcgc
    17821 aatttatagg gaataatttt actgtttctt ctgtagcttt gtttgaacct aaaagtccat
    17881 actggtcatt gtaaccacta ccatctacac ttgctgtcat tatatcatca agtgaatata
    17941 cttttttagc tccattttgt cttaaaattc tctttgttct ttgtcttgta tgtatatcac
    18001 aagttaaaat acttgttgta tagttaagaa tagtttttgg attgtttgca aatactattt
    18061 ctacctctgc accacaatcc ttgattaaat tcttataata atcaacataa tctacaccag
    18121 taaatgtatg cttgttatat ccaaatagtt ctctgtattt ttcttctgtt aaaacatctg
    18181 accaaggatt tattccctta tcatcaagtt catcaatact tattaagtga tttccaactt
    18241 catcagaagg gtagcttaac attaatacga cttttctaca tccttttgca attcctttta
    18301 aacatatagc aaaacgattt ctacttaaaa ttgggaatat aaccccaaca gtatcatctc
    18361 caaatttatc ttttacatct tttgcaatat tgtcaacgtg agcataattt ccctgtgctc
    18421 tagctacaac agcttctgta acggcaatta catctttgtc cctaacttca aagttttcac
    18481 tttttgacgc atttaaaaca gaatcaacaa ctattttaac taagtcatct ccttgtctta
    18541 taataggtgc tctaacccct cttgaaacag ttccaactaa tctatccatt atctaaaatc
    18601 ctcctaaaga attaatattg tcatattagt accatgtata ttataattca aatataatta
    18661 taagtaaaat aaataatact aatatttgac ataaggagta attatatatg aatataaaac
    18721 ttgaacttta taaggttttt aatgcagttg taaataataa gagtttttct cttgctgcta
    18781 aggaattgtt tatgtcacag ccagctgtaa gccagtcaat aaagcaatta gaagaacaat
    18841 tagatacttt attattttat agaaacaata agggagtaaa attaactcca gaggggaaaa
    18901 ttctaagtga acatgtcact actgcattaa agttgatatc atcaggagaa gatagaataa
    18961 ataaatttaa aaaattagaa tacggttctc tgaaaatagg agtaggagat acagctgcac
    19021 gttttttctt gttaaaatat ttagaaattt ttcataagaa gtatccacat atacatgtat
    19081 ctacaataaa tagaacgagt agagaactta tatctttact caaagatggg aatatagata
    19141 tagcaattat aaacatgcca atagaagatg atacactaaa tattgttgag tgtattgaaa
    19201 tacatgatat atttgtatgt gcaaatgatt acatagaata caaaggtagg aaaatatcat
    19261 tggaagaatt aaacacattg cctttaataa tgctagaaaa taaagcaaat tcaagacttt
    19321 atgtaaatga atatttttta tcaaaaggta taaaactaaa cccggatata gaattgggtt
    19381 ctcatgaatt acttttagaa tttgcgtata taaatcttgg agtgtcttgt gttatagaag
    19441 aatttagcat agattatttg gaaaatgaga aattgtttaa attagatata aaagagccaa
    19501 tacctaagag aaatataggt tactgccatt taaaagatat atctttatcc ttagcaacca
    19561 aagaattttt aagcatgata tctaataaca tataatttag aatacatatt tcacaagtaa
    19621 tttaatataa aaatagagat aaattcgtaa atttgaaaga ttcatatagt aattagtttt
    19681 tattaaaaat aaagataagg ttaatttgag aacatttata taattaatat cgtattttaa
    19741 atatgaacag tatatttaga ttaatgtata aagggggatt agaaatgtct gattctgaat
    19801 atcctcaaat gaaaacctta gaaactagaa gttgtatact aagacctgca tcattagatg
    19861 atgttgaaga tttttttgac tgttataaaa ataaaatagt agtaaaacat ttaccattta
    19921 gtgagcataa atctttagaa gatacaagaa agtttataaa atcatttttt ctaaatccct
    19981 ataaaaaagg gaaaattggc cactttgcta tagtatataa aagagacaat aaagttattg
    20041 ggaatatggg gtttaataat ataaatccta aagcattaga agcagaaata ggtatatgta
    20101 taaatccaag ctattgggga catgattttg ctactgagat aacaaaaaga gtaatacagt
    20161 atggatttag agaactaaac ttaaataaaa taattgcaat aacctatgaa gaaaaccaaa
    20221 attctacaaa atcattagat ttattaggat ttaaatgtac aggaaagtat atcaaaaaaa
    20281 ttcatactgg aaatactgta aaaaatgttc cttgttatca atatgaattg aaaaaacaat
    20341 agatataatc atttaatatc tatcctggtt atttagtaat gattgttttc taaataaaat
    20401 ttcaataaag tagttgactc aaaatataga taatcctgtt ttttattgtt aaaatactga
    20461 aaatgcattt gattttatac aaaaaagagt gtctcatgaa ctaaaaagtt tatgagacac
    20521 tctttttgaa taaatcgtat tttaattaaa atagtatata tacttaaaat attaattttt
    20581 attagttctc taaattttta aatccttctc caagaacctc atgcacatct gtaacaataa
    20641 caaatgcagt agggtcagtt cttttaacta atctctttag ataaagttct tgttttttag
    20701 aaactacgac taatagaact tctttatttt ctctagtgta tccaccttta ccatcaagaa
    20761 tagtaagacc tctatcaagt tcttcagtta tagcttgtct taaactttca ggctcatcag
    20821 agataataaa aaatgctttt gaatggtcaa atccttccat tatcatatct gctattttta
    20881 ttaatacaca aagtgctata ccagagtaaa gagcagtttc taaactacga tttactatac
    20941 cagaagatat aacaaccatt ccatctaggc atgacatgaa ttttgtagct tttattcctg
    21001 ggaatttctt actaagtatg agtgcaatta agtctgttcc tccagtagaa gcatctgatt
    21061 taaacattat tcctaaacct ataccaacta ggattcctcc tgcaatagaa gacaataata
    21121 agtcattagt aaatcttagt tttgataatg ggtctgttaa ttgaactata gctgaaaata
    21181 caactgttcc aaataaagtt tttaatgaat tttttgtacc cataattcta aaagcaagta
    21241 ttactaatgg gacacctata attaacatga tagctgatac tggaagtcct gtaaccttgt
    21301 ttagtactaa tgatagacca ctaagtcctc caggagcaat tgtatgtggt tttaaaaaca
    21361 tatttatacc aatactcatt gatatacaac ccaaaaatag tccaagcatt tcaagtaaaa
    21421 ttggcgactt ttgttttttc atataaacac ctccatgaac ttgtcacaat ttaatattat
    21481 aacatatttg accagtttat attatataaa atttcttgat ataagcaatt tcaagtattt
    21541 cattattctt aatatttttg aatttttata tagtgttttc atatctttac tgatgttgtt
    21601 acactcctat gaagtttttt atatattata atttttttat tcttttataa agtatcccct
    21661 ataaagatgg ttctatgaaa attttctcta tactgtttaa cgtatataga gattttgtgt
    21721 ataattataa ggtatttaat ggaaaaaatt atcttgcttt tatatgttat caaaaggatt
    21781 tattaaatac aacagacagc ttgataacat ataattatat aaatataatt tatg
//
LOCUS       DAMKPG010000066        21563 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMKPG010000066 DAMKPG010000000
VERSION     DAMKPG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21563
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.069"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            76..1527
                     /locus_tag="PC621_002516"
     CDS             76..1527
                     /locus_tag="PC621_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF4109663.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1747..2208)
                     /locus_tag="PC621_002517"
     CDS             complement(1747..2208)
                     /locus_tag="PC621_002517"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF4109664.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2314..3507)
                     /locus_tag="PC621_002518"
     CDS             complement(2314..3507)
                     /locus_tag="PC621_002518"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF4109665.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3530..4195)
                     /locus_tag="PC621_002519"
     CDS             complement(3530..4195)
                     /locus_tag="PC621_002519"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF4109666.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4229..5332)
                     /gene="ribD"
                     /locus_tag="PC621_002520"
     CDS             complement(4229..5332)
                     /gene="ribD"
                     /locus_tag="PC621_002520"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF4109667.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5464..5585)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5774..8257
                     /gene="recQ"
                     /locus_tag="PC621_002521"
     CDS             5774..8257
                     /gene="recQ"
                     /locus_tag="PC621_002521"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDF4109668.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GANILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVMLDSYLENLKNNIIAE"
     regulatory      8345..8456
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8598..9899
                     /gene="thiC"
                     /locus_tag="PC621_002522"
     CDS             8598..9899
                     /gene="thiC"
                     /locus_tag="PC621_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF4109669.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9953..10147
                     /gene="thiS"
                     /locus_tag="PC621_002523"
     CDS             9953..10147
                     /gene="thiS"
                     /locus_tag="PC621_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF4109670.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10140..10943
                     /gene="thiF"
                     /locus_tag="PC621_002524"
     CDS             10140..10943
                     /gene="thiF"
                     /locus_tag="PC621_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF4109671.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            11006..11776
                     /locus_tag="PC621_002525"
     CDS             11006..11776
                     /locus_tag="PC621_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF4109672.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11793..12896
                     /gene="thiH"
                     /locus_tag="PC621_002526"
     CDS             11793..12896
                     /gene="thiH"
                     /locus_tag="PC621_002526"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF4109673.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12897..13586
                     /locus_tag="PC621_002527"
     CDS             12897..13586
                     /locus_tag="PC621_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF4109674.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13774..15168
                     /gene="yfcC"
                     /locus_tag="PC621_002528"
     CDS             13774..15168
                     /gene="yfcC"
                     /locus_tag="PC621_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF4109675.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            15685..17052
                     /locus_tag="PC621_002529"
     CDS             15685..17052
                     /locus_tag="PC621_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF4109676.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            17077..17685
                     /locus_tag="PC621_002530"
     CDS             17077..17685
                     /locus_tag="PC621_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF4109677.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            17733..18104
                     /locus_tag="PC621_002531"
     CDS             17733..18104
                     /locus_tag="PC621_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF4109678.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            18283..18621
                     /locus_tag="PC621_002532"
     CDS             18283..18621
                     /locus_tag="PC621_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF4109679.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            19088..19591
                     /locus_tag="PC621_002533"
     CDS             19088..19591
                     /locus_tag="PC621_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF4109680.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            19638..20597
                     /gene="moaA"
                     /locus_tag="PC621_002534"
     CDS             19638..20597
                     /gene="moaA"
                     /locus_tag="PC621_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF4109681.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            20601..21071
                     /gene="moaC"
                     /locus_tag="PC621_002535"
     CDS             20601..21071
                     /gene="moaC"
                     /locus_tag="PC621_002535"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF4109682.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            21107..21538
                     /locus_tag="PC621_002536"
     CDS             21107..21538
                     /locus_tag="PC621_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF4109683.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         8299 a         2336 c         3742 g         7186 t
ORIGIN      
        1 aacaaggaat tattcatttt tttgatttta agatttattg agaaaacaaa actaaatagg
       61 ggggtaatta atataatgaa ttttttaaat ggtattttaa cgagtattaa caatgcagta
      121 gtatttgtaa acaattactt atggtcgtat atcttaatta taatgttagt tgtaattggt
      181 atttacttta ctattaaaac aaattttgta caattcagat attttataga aatgtttaga
      241 cttctgggtg atggtacagc gaataaaaat gcaaaaaagg aaggaaaaat ctcttcattt
      301 caagcatttt gtataagtac atcttcaaga gttggaactg gaaatatagc tggaatagca
      361 attgcagtgg tagcaggggg acctggtgct gtattttgga tgtggcttat agcattaatt
      421 ggttctgcat caagctttgt tgagagtaca ttagctcaga tttacaaagt gaaagatggt
      481 caagctttta gaggtggacc agcgtattat atggaacaag gtctaaataa aaaatggatg
      541 ggtattttat tctcaatact tataactata tgttatggtt ttgtatttaa tgcagttcaa
      601 gcaaatactg tttcacttgc atttaataat gcatttggaa taagtaaaat ggctatggga
      661 ataggtcttg gaattttaac tgcaatagtt atttttggtg gagtgcatag agttgcaaaa
      721 gtttctgaaa taatagttcc tgtatttgca ggattatata tattagttgc tttggctata
      781 gttgtaatga atataacaga gataccaagt gttatagcat tgatagttga aagtgcattt
      841 gattttaaag gaatggcgat tggtacattt atgggagttg taatgactgg agtgaagaga
      901 ggtctatttt ctaatgaagc aggtatgggt agtgctccta atgctgctgc aacagcacat
      961 gtaactcatc ctgtaaaaca aggtttaatt caatctttag gtgtttttac agatactata
     1021 ataatatgta gttgtacagc attcatggta ttattatatt caggttatag tagtagtgga
     1081 gcaactggaa ttgaattagc tcaagaagct ttaactcatc atataggacc agttggaaat
     1141 atatttatag cagtttgtat attcttgttt gcatttagtt ctatagttgg taattactat
     1201 tatggtgaat caaatatgga gtttatgagt ggaagtaaga ctaaacttaa tgtatttaga
     1261 gtttttgttg taggcatggt tttatttggt tcacttactc aagtagatgt agtttggaat
     1321 cttgcagact tatttatggc attgatggcg ataataaact taatagcaat agcactttta
     1381 ggaaaatatg catttattgc tttaaaagat tatacaagtc aaaaaaaatc aggaataaaa
     1441 gaccctgtgt ttattgcaga tgaaatagaa ggcctagaaa aggtatcaga gtggcatagg
     1501 gaattatctg aggaacattt aggataaaaa atagtataaa attataaaac cattttgaaa
     1561 gactagagta tgtatatgct ttatatgaat ataatattga attaatttat attactggaa
     1621 tttttaattc cagtaatatt tttttattat ataatttagc tttattaaaa gtttttatta
     1681 ttaaaaatag cttatttatc gtaaagtgaa tgatattggt tttttaatta ttatgaatgt
     1741 tggtatttaa tttaaagact taaataaatt tgccatttct agcgcattca tagcacaatc
     1801 ataaccttta tttccagctt ttgtacctgc tctttcaata gcttgttcaa tattttctgt
     1861 agttacgatt ccaaatataa caggtaattc actgtctaaa gatacttttg ctatcccttt
     1921 agagacttca ctacatacat aatcataatg agaagtagcc cctcttataa cacagcctaa
     1981 acagattata gcatcatacc taccactttt agccattttt tgagcaacaa gaggaatctc
     2041 aaaagctcca ggtacccaaa ctatttctat attttctgga gaaacgtcat gtcttaaaag
     2101 acagtcttca gctcctgata aaagttttga tgttataaat tcattaaaac gagaatttat
     2161 tatacctatt tttaaatctt ttccaataag ttttccttca tatatcattt ttatttcctc
     2221 caattttatt tatgttatag atttatattt ataaatatct ttaattttaa ttataaaaaa
     2281 ctacttagta gaaaaatatc atcatataat catttatata atatctaaaa gatgtcccat
     2341 tttatctttt ttagttttta aatatagctt attaaagtca ttaatttctt cattaatagg
     2401 gattctattt tcgattttta ttccatattt ctctaattga tttattttat ccgggttatt
     2461 acttaaaaga tttatacttt ttatgtttaa attttttaat atttgagcag ccatatagaa
     2521 atctctcata tcttcttcaa atccaagcat taaatttgct tcaacagtat cgtaaccttc
     2581 ttcttgaagt ttataagcct taattttatt taaaaggcca atacctctac cttcttgctt
     2641 catataaatt aaaactccac ttccattttc cacaattttc ttcatagaag attctagctg
     2701 taatccacaa tcacatttta gagagtggaa cacatctcct gttaaacatt cagaatgaag
     2761 tctaactaga gtattttcta tatttatatt tccataagta agggcaatgt gttcttcatt
     2821 tgaataagta tctctatatc ctatgatttc aaaatttcca tattttgttg gaaggaaagc
     2881 actagatact ttttctatag tagtttcatt tattttgcgg tattctataa ggtcttttat
     2941 agtgatgatt tttaaattat gcttttcttt gaaaatcatt aaatcatcaa ctcttgccat
     3001 tgtaccatca tctttcatta tttcacagat aagaccaatc tctttaaaac ctgcaagtct
     3061 agcaaggtct actgaggctt ctgtatgtcc atccctgaca agaactccac cagtttttgc
     3121 tattaaagga aatatgtgac caggcgtttt gaaatcttta cttgtagaat tttcatcaca
     3181 gaacaatttt acagtttcag ctctttcata tgcagatata ccagttagag tatctgctcc
     3241 atctatagta acagtaaagg ctgtttgaaa tgtatctgta ttttctctaa ccataagagg
     3301 tatattaaga cttttaaatt ttttttcagt ggcaggtaga caaattagac ctctaccata
     3361 tgtagccata aaatttattg attcaggagt agccatttca gctgccatca ataaatctcc
     3421 ttcattctct ctactttcat catcaactac gattaccatt ttcccatttt ttatatcttc
     3481 aatagcttct tcaatagtat taaacatgta attcactcct ttattatttt taaaaaccat
     3541 ttttaaataa aaaatcttta tctatatttg atttatggtt actactatta gaatttaatt
     3601 ccttgtagtt attagccata aaattattaa catactttcc aattacatcg ctttctaaat
     3661 taacaaaatc tccaacattc tttgtcaaga gaatagtgtt aattttagta tgaggtataa
     3721 tagaaacctt aaatatttca tcatctaaat atgatatagt taaacttaca ccatcaatag
     3781 caacagaacc tttgagtatc attgaagata aaagtttttt atcaggtcgt atacttacta
     3841 aaactgcatt ttcatctttt tcaaatttgc agatagttcc tttaccatca acatgacctg
     3901 ttactatatg accaccaaat cttgaagaaa gactcatagc tctttcaaga tttaccttat
     3961 catttgcatt taataaacct aaatttgtgg aacgaattgt ctccatcata acatcggctg
     4021 taaattcatt ttttccgagg tttgaaacag ttagacaaac tccatttacg gctatactat
     4081 ctccaagatt tgtgccatct aatattttat ttgatagtat ggttactttt ccactcttac
     4141 cattagcagt aatttttcta agtattccaa cttcttctac tatgccagta aacatcctaa
     4201 cctcctatat gaatgtatta acaaaaattt aatttaaaac atctgattct ataagaatat
     4261 cgccatctat caaagttaca ttattgattg ttaactgtgt tgaatctgac aaatgattta
     4321 ttccttctcc agaaacgaat gtaggtgcat ctttaccacc tataatcttg ggtgatagaa
     4381 ataatttgac tttatcaact aacttatttc taaagagact gtcattgaga gtagcaccac
     4441 cttcaactag aacagaatca atgtttaact ctcctaattt atacattaca aattccaaat
     4501 ctactaagtt gttttttgaa ggagaaatta ttgtttcaac attattttct tttaacatag
     4561 ataattttat actttctttg caacatgtga aaactatagt tttactggtt ttatctttaa
     4621 ccaaattact attaagtggc aactttaggt gtgtatctat tactattctt gtaggatgag
     4681 acactttttc ttgattgagc ctgcaagtta gtaaaggatt atcgttcaaa actgtattta
     4741 tgccaaccat tatactttgg tatttatttc tgtatttatg agttagatac cttgatgatt
     4801 cattagatat ccattttgat tctagtgatt ttgtagcaat tttaccatct agacttactg
     4861 ctgattttac aatacaaaga ggtcttttgt tttttatatg ataaaaaaat gcctcattta
     4921 aatctcttgc ttcatcttct aatattccca cagtaacatc aatattgtta tcttttaatt
     4981 tttttactcc attaccacaa acaagtggat ttgggtctaa tgtagatatt acaactctct
     5041 taatcttatt ttgtataatt ttatctacac aaggaggcgt tttcccataa tgagaacatg
     5101 gttctaagtt tacataaaga gtagagtctt ttagactttg tttagcactg tttatagcat
     5161 ttacttcagc atgattagag ccaaatttct catgatatcc tttgcctatt atattactat
     5221 ctttaacaat tacacaccca actaatggat ttggatttac aaagccttct ccatttttag
     5281 ccaattcaat agcttttttc atataataaa tatctttttc tttttgattc acaatatgta
     5341 cacctcctgt ttaattggat aatttttaaa caaaaaaccc tgagaattaa ctcagggttt
     5401 agaataatct acaataaatg tttaataaaa gtagaagaaa attgctctac aaaaacactc
     5461 attaaatatc ttcttccatc cagactttac tgtcggcttt ggaatttaac caaatcaacc
     5521 aagttttacc ttggctcgcg ggctataccg ccgattggga atttcaccct accccgaaga
     5581 ttaagtatta aatttatatt tagaatgtat cataaattat tttactttgc aaactatttt
     5641 tttaaaaaat atatgttaat atataatata gaatgtctat aaactttgaa ataccacgat
     5701 tgtgaatata aaaataaaaa tttgaacata atcaggaaag aagtctaatt aatttaaatt
     5761 aggagagtaa tatatgaata aaaaactttt agaaatacta tcaaaatact atggatatac
     5821 aagttttaga aaaggtcagg aatctataat aaattctatc ttaagcgaaa aagatgtgtt
     5881 agctattatg ccaacaggag gggggaaatc aatatgttat caaattccag ctttgatttt
     5941 agatggaatg acaatagtaa tttctccact tatatcactt atgaaagacc aagtagatgc
     6001 cttaaagact atgggaatta gtgctgcttt tataaatagt tcattaagca gcaaagaatt
     6061 taatgaaata ttaaataata ttagaaaaaa taattataaa atactctata ttgctccaga
     6121 gagattagat gcacaagagt ttattgagct aataaataat aacaatgtaa gtcaagttgc
     6181 gatagatgaa gcacattgtg tatctcaatg ggggcatgat tttaggttga gttatagaag
     6241 gatttctgat tttataaaaa atttgcctaa aagacctata gttacagcat ttactgcaac
     6301 agcttcagaa gaggtaagaa ctgatataat aaatttactg tgtttagaaa atccagatta
     6361 ttatataact ggatttgata gagagaattt atcaataaat atagtaaaat catctagtaa
     6421 aaataagtat attttagatt atattcaaaa tcataaaaat gagtctggaa ttatatatgt
     6481 tgcaactaga aaggaagttg aaaatatata taatggttta tctaagagaa atatatctgt
     6541 atcaaaatat catgcaggtt tatctaaaaa tgaaagaagt aaaaatcaag aagattttat
     6601 aaatgataat gtggatataa tggtagcaac aaatgcattt ggaatgggta ttgataaacc
     6661 taatataaga tgggttatac attacaatat gcctcaaagt atagaaaact actatcaaga
     6721 aataggtaga gctggtaggg atggagaaaa aagtgagtgt gtacttttat tttctccagg
     6781 agatgtacat atacaaaaat atttaattga tattggagtt gaaaatcctg aaagaaaatt
     6841 atttcagcat aagaagcttc aatacatgat tgacttagtt tacagtaatt catgctatag
     6901 aaagacaata ttgagttatt ttggagaaaa ttatatagac aattgcaata attgtagcaa
     6961 ttgtttagta gagggtgaaa tagtagataa aactatagat gcacaaaaag tcatttcatg
     7021 tgtagctaga atgaaaagaa gttatggtgt tactactatt gtagatgtac ttagaggttc
     7081 taagaataaa aagattttac agcttggatt aaatactttg tctacatata acattatgag
     7141 agattattct agtgaagact taaagaattt tgtaaataca ttggtttctc atggtttttt
     7201 agatttagtt gagactctag gcaatggaaa tagaggtagt tttcctacta taagattaaa
     7261 tgagatgtct atgaaagttt taaaaggtga agtgaaagta gagtttaaag aaattgttat
     7321 gacaaaatca ttggaagtag aggatgagtt atataatgct ttaagagaac ttagacattc
     7381 aattgcaagt gaagaaagga tagctccata tatggtattt ggagatggta cacttagagc
     7441 catgtctagt agttatccaa ttaataaaga agaaatgcta gatatctctg gagtaggaga
     7501 aattaaatat caaaaatatg gtagagattt tgaagcagta attaaagaat atgtcgaaaa
     7561 aaataatata aataaaaaaa tagatgaaaa aaatgtcgat ttaaagaacg ttaatagtga
     7621 tttcttagag gttaatacag ataaagtttt atatgaaaaa ctgttagtca ttagagaaga
     7681 atatgcaaca aaggaaaaat tacctgcata tatgatttta gccaataagg ctttgaaaga
     7741 aataagtgga agatatccac ttgatgaaga acaattaaaa gatatttctg gcataggtgg
     7801 agtaaaattt gaaaaatatg gagctaatat tttggcagag gttaataaat atgtaaaaga
     7861 aaataatatt aatgttattt gggagaaaaa gggcaaaagg aagttaatat tagatggaga
     7921 aagtagaaaa aataatgaaa tagcattaga tttgctaaat caaggaaaag atatacaaag
     7981 tatcagtcaa gaaatagaag tttcattgtc aaccattatt ggatatgtgc atgactatgt
     8041 aaaggatgga catagtatat catttgaatt aggactagaa aatttttata atgaagataa
     8101 aaagaagatg attttagaag tttgcgaaaa agttggctat gataatttaa ataacataaa
     8161 aagaaaactt ccagattcaa taaagtatga aaatataaga gctgtgatgc ttgactctta
     8221 tcttgaaaac ttaaaaaata atattatagc agagtaattt ttaacttaat aaaaataaaa
     8281 tttgtattat tgtgttgaac taataatttt gctgtgttat actgaatttg taaaaataat
     8341 gaaaaattag agctaggggc gctagaattg gctagctgag agataaagcc gtaacttaca
     8401 aaccctaata acctgatcaa gataatgctt gcgaagggaa gctttttaat ataattttat
     8461 tatataaata ggcaaattat tcaatgttag gagaaatttg tctaagtgta tatacgatta
     8521 taagagtgct ttccaaacga ggaaaagcac tttttttatt ttcaaaaact ataactttaa
     8581 tcttggagga ttgtaatatg aactatacaa cacaaatgga tgctgctaga aaaggcataa
     8641 taactaaaga aatggaaatc gtttctcaaa aagaacaggt agatgtaaat gaattaagag
     8701 aattaatagc aaatggacaa gttgttatac cagctaataa aaatcataaa tctttaagtg
     8761 cagaaggtgt aggtaaaaat ttaagaacaa aaataaatgt aaatctagga atttcaagag
     8821 attgtaagga tatagaaaaa gaacttgaaa aagttagagt agcaatagac atgaaagctg
     8881 aagctataat ggatttaagt aattatggca aaactagaga atttagagaa aaggttgttg
     8941 aaatgtcacc tgcaatgata ggttcagtac ctatgtatga tgcagtagga tatttggaaa
     9001 aagaattaaa agatataaca gaagaagagt ttttaaatgt aataagacaa catgctattg
     9061 atggagtaga ctttattact atacatgcag gtcttacaag aagtgtttgc caaaaaataa
     9121 aaaatcatga gagattgact catatagtat ctcgtggagg ttctttattg tttgcatgga
     9181 tggaattaaa taataaggaa aatcctattt atactaattt tgataaaata ttggatatat
     9241 gtgaagaata tgatgttaca ttgagtttag gagatgcttg tagaccaggt tgtataaaag
     9301 attctactga tggtgttcaa attcaagaac ttgtagtatt aggtgaactt actaaaagag
     9361 catgggaaag aaatgtacaa gtaatgatag aaggtcctgg acacatggct attgatgaaa
     9421 tagaagcaaa tgtagtatta gaaaagagat tatgccatgg agcacctttt tatgttcttg
     9481 gaccattggt tacagatata gcaccaggtt atgaccatat aacaagtgca ataggaggag
     9541 ctttagcatg tgctaaagga gtagattttt tatgttatgt aactccagca gaacatttaa
     9601 gattgcctaa tttagatgat atgaaagagg gtataatagc tgcaaagata gctgctcatg
     9661 caggagatat agctaaaaat gttaaaggtg ctcgtgaatg ggataataag atgagtaaag
     9721 ctagagctga tttagactgg tgtgagatgt ttagacttgc aatagaccct gaaaaagcta
     9781 agagatacag agatgaatca actcctactc atgaggatag ttgtactatg tgtggaaaaa
     9841 tgtgctcaat gagaacagtt aagaaaatat taaacaatga ggaacttaat ctaatataat
     9901 ttttggctag tatgactgga ataaaaagaa gctgaaaaga ggaggtgatt tagtgaaggt
     9961 taatgggaag gaaattgaat ttgaaaaaga tttgactgtt atagatttat taaataaata
    10021 taatttaaaa agtgatagag ttgtagttga agtgaattta gagataattg aagaatctaa
    10081 ctacaataca tatgtattaa aagacgagga cattgtagag cttattagct ttattggagg
    10141 tggttaaaat aaaaatattt gtaaatgaat tattaacaga tgttgaggaa aatacaactg
    10201 cttataaagt tagagatagc tataacaata aatgtgatgt attggtgtta aatggatatc
    10261 caattaaaac agatatgccc ctttgtgaaa atgataaatt aacacttata caaaaaggag
    10321 ttaagccatc acttgatgaa ttggaaagct tgttgattgc tagacatact ccaaatatac
    10381 ataataaatt aaaaaaaggg aaagttgcta tattgggatt ggggggactt ggttccaata
    10441 tagctatctc tttagctaga ataggagtgg gagaactttt acttatagat tatgatgttg
    10501 tagaaccatc taatttaaat agacaacagt attttataga tgatatagga aaactaaaaa
    10561 ctgatgccat gatagaaaat ataaaaaaaa taaatccatt tataaaatta aataaacgag
    10621 atattttttt aaacaagaac aatatggata ctattaagga ttcagattta ataattgagg
    10681 cctttgatga tgcttcatgt aaagcacagg tatgtaatta tgtattaata aatttaaaag
    10741 ataaatattt aatagcatca tctggtatgg caggttacta tgattcaaat ataataacta
    10801 ctaagaagat aaaagacagg ttttatatat gtggggattt tgtgaatgaa gctaaatttg
    10861 gtgaagggct tatggctcca agggttgcca tctgcgcaaa tcatatggca aatttggcaa
    10921 cgcaaatact tatagatatg taaatattaa aattagatat aaaaaagatt gaatattaaa
    10981 aaaatttaac gtggagggaa ataatatgga taaattggta cttggtggac atgaatttaa
    11041 tagcagatta ttggttggaa caggtaaata tggttcaaat aatatattgc ctgaggtaat
    11101 aaaagaaagt ggaagtgaaa taataacaat ggcacttaga agagtggatt tagacaataa
    11161 acaagaaaac atattaacgt atattccaaa agagatgaca atacttccaa atacatcagg
    11221 agctactaat gcagaagaag ctgtaagaat agctagaata tctagaaaaa tgggatgtgg
    11281 agattttata aaaatagaag taatatcaga tactagatat ttacttcctg ataatgaaga
    11341 aactataaaa gctacaaaga tacttgctga tgaggggttt atagttcttc catatatgac
    11401 accagacctt tatgctggaa gaagacttat agaagctaat gcagccgcag ttatgccact
    11461 tggagctcca attggttcaa atagaggtct tcaaatgaaa gaaatgataa gaataatgat
    11521 agatgagttg gatataccaa taatagtaga tgcaggtatt ggaaaaccat ctcaagctat
    11581 ggaggctatg gaaatgggag ctgatgcagt acttgtcaac actgctatag cttcagcagg
    11641 agaccctgta caaatggcta gagcatttaa attagctgta gaaggtggaa gagaagcata
    11701 tattgcaaaa acaggaaatg tgtcagaatt tgcaaatgca tcttctcctt taacaggttt
    11761 tttaggcaat ttatagaaag tggtgattgg tgatgagttt ttatgatgta atagaaaaat
    11821 atagggattt tgattttgat ggatatttaa ataatgttac tgataatgat gttttaagaa
    11881 gtttatcaaa agacaagctt gaagattttg atatattaaa tttgctttct aaaactgcag
    11941 taaaacattt agaagatatg gcacaaaaag cacataaatt aagtgttcaa tactttggta
    12001 aaacagtatg tttatataca ccaatgtata tagcaaatta ttgtgttaat caatgtgttt
    12061 attgtagcta taatataaag agtggaataa aaagaaaaaa attaactatg gatgaaatta
    12121 gagaagaggg agaagcaatt tcaaaagaag gtttcaaaca tctacttgtt ttgactggag
    12181 aaagtagttt tcactcaagt gtagaatata taggtgaggc tatagaaata ttaagagaga
    12241 aatttccctc tataggtata gaagtttatc caatggaagt agaagaatat aagtacattg
    12301 tagataaagg tgttgaagga ttaacagtat atcaagaaac ttatgacgag gaaatatata
    12361 agagagtaca tataaaaggc ccaaaatcaa attatgaatt tagactagat gctcctgaaa
    12421 gaggggcaaa agcaggtatg aggactttat caataggtgc acttttaggt ctaaatgatt
    12481 ttagaaaaga aacttttttt acaatattgc atgggaaata cttaaaaacg aaatatcctc
    12541 atattgagct ttcatattca acaccaagaa tgagaccttt taaagggtgt tttgaggaat
    12601 tggtagatat aagtgatact gatttagttc aagctatggt atgtatgaga ttatttgacc
    12661 ctcatgcagc tataaatata tccactagag aaaatttaga aatgagaagt catataatac
    12721 ctttaggagt aactaaattg agcgcaggtg tatctactga tgtaggaggt cattctcaag
    12781 atgaacatga tactgctcaa tttaagataa atgatgaaag tactgttaaa gatgtggaga
    12841 aaatgctaaa ctcaattggt tatcagcatg tgtttaaaga ttgggaaaga ttttaaatgt
    12901 atctgataac aaacagaaaa ttatgtagtg aggaacgata tttagaagtt attaaagagt
    12961 ctatattgag tggtgtagaa aatataataa taagagaaaa ggatttggaa tatcaagagc
    13021 taagaaaatt atatatgaaa ataaaaacta aaatcaattg tatagatttt caagagcaaa
    13081 tatcagatga aagtttgaaa actaatataa atcaaaaaga gtgtagaaat aaattcaagg
    13141 ttaattttat aataaatagt aacattgaat tttttgaaaa agtggattgt caaggtatcc
    13201 atcttccttt taaattattc ttgaatttaa ttgaaaataa gtataatttt aatgaaaata
    13261 aaatactagg attatcttta cataaagtag aggaagtaga ttatttagaa aaattaataa
    13321 gaaatcaaaa tataaaaata gattacataa cattatcaca tatatatgaa actaaatgta
    13381 aagaaggatt aaatccaaaa ggaattgagc ttttaaaaga agctaaaaag attactgata
    13441 ttaaaataat tgcacttggg ggaattttac catctaatgt caaagaaaca ttaaaatact
    13501 gcgatgattt tgctataatg tcaactataa tgaggtctaa agatattaaa aaaaccatat
    13561 caaattataa tgaaaagttg aattagtaaa tatagtatat tgaattttta acttaaataa
    13621 aaataaaaaa gttatctttt aaattttaaa aggtagcttt tttattttat atctttgcta
    13681 ataaaaaatt gtaaaaaggg aaaactttag tcagagagat taaacaatat cctagaaaga
    13741 tttaaaattt agtaaagtat ggaggacatt ataatgactg acagtaaaaa gaaacgattt
    13801 attataccac acacttttac aataatattt ttactaatag tcttgatggc aatattgact
    13861 tggatagtac caagtggtga atttagtaga gaagatgtag aaggtagaat ggtagttgta
    13921 ccagggactt atcaaagtgt agaaagtaat cctcaaggat ttgcagatgt ttttaaagca
    13981 ccaatagaag gctttataga ttcagctgaa gttgtaggtt ttgttttaat agttggaggt
    14041 gcttttggta ttgtaaatag gactggagct atagaagcag gaatagcttc ggttatctct
    14101 aagtttaagg gaaaagaact tcttataatt ccaatatcaa tgattttatt tggacttgga
    14161 ggtacaacat ttgggatggc agaagagaca ttaccatttt atatgatatt tgttccactc
    14221 atgacatcct tgggatatga ttctctaaca gcaatagcaa ttgtatttat aggagcagca
    14281 gcaggagccg ccgcttcaac aataaatcca ttttcagttg gtattgcaca ggcactagct
    14341 caacttgctc ctggttcagg agtaacttac agagtagtta tttttatagc atatatacta
    14401 ataagtatag catttgttat gtactatgct aagaaggtaa aggaaaatcc tgaaagttca
    14461 atagtatatg aaatagataa aaataataaa tcaatactta atcaaacttc aacagaaata
    14521 aaagaattta cttttaaaga agtatctgtt cttgctatat ttggaatagg gatggcaata
    14581 atgatttggg gtgttttaag tcaaggatgg tacattccag aaatatcaat gtgttttgct
    14641 gcaataggtg tgctttctgg attggttggt agattatctc aaattgaaat ctcagaaagt
    14701 tttatagatg gagcaaaaga tttagcatct gcagctcttg ctatagcttt ggctcgtgga
    14761 atcgtaataa ttgctcaaaa tggatttata atagatacta tattaaattc cgcagcagcc
    14821 tttctaggtg ggcttccaaa ggttatattt gtatatttat catttttctt ggaagctgca
    14881 ttggcatttt taattccatc atcttcaggt cttgcttcac ttacagtacc tgtacttgct
    14941 cctcttggtg atttggttaa tatatcggct caagtgatag taaccgctta tcaatttggt
    15001 gtgggtctta caaactttat aactccaact tcaggagtac tgatgggagc acttgcagta
    15061 gcccatattc cattttcaaa atgggtaaga tttataatgc ctatcttgtt gattttgaca
    15121 gctgtatctc tagtcttttt aacaataggt atttatatag ggttttaatt aagtgtagtt
    15181 ttatttaaga tatttagtaa tggtataaaa tgataatatg ttttcaataa attgccacta
    15241 atttaattag tggtaattta ttttttgtat ttaaaaataa atgtatttac ttgtattttg
    15301 aattaatatc tacttagaca ttgttttaat tgttatattt tcatttaatt aagcattgat
    15361 ttatcgtttt ttatatttca tataacaatg caaaaaataa tttttatata gtaaatgaca
    15421 aaagtttaca ataaatgtat attttcttac tcattcctta aaattactca tttagtttaa
    15481 taactctcat ttcatatgta aagttggtaa aaaaattaaa attaaaagta aaaaactaaa
    15541 taaattaatg ttaatttgaa atatatatga aggtttttgg agaatactta cttatgatag
    15601 gaaaaaatgt taaaataata gtgtaatcaa atataaagga ctgaaataat taacagttat
    15661 taaatattag gagtgtaaaa atttatggga ttaaaagaat ttgattttat tgaagagtct
    15721 attgatatgt tgaggacaat gtcaccaaca ttagaaacta tatcagatga aatagaagag
    15781 tattttgaaa atatcttgga tgaaaagaat caagaatata taaatgtaac ctctagaata
    15841 aaatcagaat caagtttaag agaaaagata attagaaata gatatttaaa aaaatatgga
    15901 gaagcaagta atcttattca taatgtttct gacttaatag gcctaagaat tgagtgtaga
    15961 tttattgagg atgaaaacaa aatatataga cttttgagaa gatattttaa taaaacagat
    16021 gacaaaataa actattataa taaagagaat aagaatatta aattaaaact atctgagagg
    16081 caacccaata agcaaaaaaa tggatttgaa atatataaaa tagatggagt ttttgcgtat
    16141 ttagatagag aagtaaaatt tgaacttcaa attaaatctc ttgtaaatgt gttttggagt
    16201 gaaatagaac ataaaataat ttataaaaat aatacatatt tacttgctga taaatttata
    16261 aaggatatga tggactctat aaagaataat cttactatga tagataatca gcttttaagt
    16321 atttataaaa actttcattc agggaagtct tttaatatga aagtaagtaa aaaagaaatt
    16381 gaaaaattat ttgcgaaact agtctatgac gctttttctg aaaagatgaa taaaagtata
    16441 ggttttgttg tagattttaa aaaaccatgt gaaactatat tgaactattc ttttaataaa
    16501 caagatataa gtgatgaagt tttaggtaat tttatgcttg atgagtttgc aagattaaat
    16561 gaaattgtaa acaaggatat agattttaat gagcaaatag aatttgaaag agaaccaaca
    16621 tttgatgata agttttgtaa agacctagga aatcatttta gaagtagatt aaacacagaa
    16681 tttccatgga acctattttt tagaatatta tttgaaatag agccatataa caataccaaa
    16741 gactttgaaa actttgtaga gtttataaaa gaaaatgtgc tactagaaga aaatagggag
    16801 caattattat gccaatttga agaagatagc aagcttataa ttgaagatat atatgattgt
    16861 atatttaata gtataagtga gattgatagt gttgaaattt tatataacta taatcttgaa
    16921 aaaataaact ttacatcaag tgaaatcata aattgtatat gtagagaata tgagtgttat
    16981 ggtgaatact tagaagaaaa agaaaagctt atgaatacat ttaaagaaaa gattattcaa
    17041 atctttgagt aataggtcaa tgctgaagtg gcgctaatgc gccatttttc aaatatatat
    17101 gaattaagta aaattatttt ggaggaggcg atttgtatag agaaaacagg tgtaattttg
    17161 gcagggggaa aaaattctag aatgggtaga gataaagcct ttttagagtt acatgataag
    17221 ctttttattg aaattgcaat agaagcattt aaaaactttg atgagttgat aataatatct
    17281 aataatgaag aattatattc taaatacgat attaaagttt ataatgatat agtaaaagat
    17341 gttggtccaa ttggaggaat atatactgct cttgaatatg caaagtatga tatagttaca
    17401 attgcatgtg atatgccata tctaaataat caaatagtcg aaagaatagc taataaaatg
    17461 aatgataaaa gtgtgatttc agtgacaaat ggaaagttac aaccattgtg ttctggatat
    17521 aaaaagagta taataaataa agtatcttta tgtataaggg aaaatgattt aaagctcaga
    17581 agctttatag ataaaataga taaaagttat atatactttg atgaagaaga tttattttta
    17641 aatgtaaaca ctgttgatga gtatgaaaaa ttaactaaag tttaattagc tatggttgta
    17701 aaatagagta ggatttatat ggaggagagt ttatgaattt acttaaatgc gattcttggg
    17761 cagtaatatt aaataatagc gataaagaaa gtaaagcata taaaatatta gatgaactta
    17821 aaaggaatat gtacaaagtt gttgctatag atgaagagaa aaaacctatt gaaggaatag
    17881 atgtgtatga atgtttaaaa gatgttccac acaatattga tgttgtagct attattgata
    17941 aacaatctaa gatggatgtg attttagaag aagtagagct tttggatata caaaatatgt
    18001 ggtttgaaaa aggaagcttt agtgaaatga tgattaaaaa gactaaagat ttaaaattaa
    18061 atatagaata taatttaagt ttatatgatg agttgactag ataaaattat atttcttaat
    18121 aataaaaata taattagtta ataaaacata ttattttata gtatagattg gttaataaaa
    18181 gtacagattg gttaatgaaa cattaaatta caatatttgt taaaagaatt gtgagttaca
    18241 tgatataatg tattaaatag gatactaagg agttgatata ttatgttaat agtaactaca
    18301 gaaaaagtag aaggtaaaaa gatatcaaag gttttaggat tagtgagagg aagtacaata
    18361 agagcaaaac atgttggaaa agatatagga gcaagtttta agaatcttgt tggaggagaa
    18421 cttactggat ataatgaaat gctcactgaa gcaagacaaa ttgccatagg tagaatggtt
    18481 gaagatgcag aagctaaagg tgcaaacgca gtaatagcat ttagactgtc ttcagcttca
    18541 gttatgcaag gggcagcaga aatgcttgct tatggaacag cagttgtttt agaagatgat
    18601 aatagtattc ttgaaaaata aaatataaat agttaaaaag aggcatcttt taaaaatttt
    18661 atggatgcct ttttttattt gaaaaaacta atttgagaag aaattttaac tattatgaca
    18721 ataaaattag ggtgatatta tataaataag taaaattata ttatataatg tatggtatag
    18781 actttaaaaa ttttatttgt agagaaaaat atggacaatg ttataataat tatagaaatt
    18841 aaaagtactt acattcatta tgtatgtgac atgtataatt agttttatat ttaatacagt
    18901 tttgaagtaa tatcttcatt ttgagaacgt tggaataata aaaaatgtat gtattatagg
    18961 tgatataaat aaaaaataaa tataatatgt tatattgctg atagaaaaga gaataaaaga
    19021 aaataggttt tattaaaaga gatttttatt tataggactt aatcaatgta tagaggggtg
    19081 agaacatatg tttaatgttg caattataac acttagtgat aaaggatatg aaggaaaaag
    19141 agaagatata acaggaaaaa agttaactga atttgttgaa aatacaggag cttataaagt
    19201 tactgagtat gtacttataa aagatgataa ggaaatgcta aaggaaaata ttataagact
    19261 ttgtaatagt aataaaatag atttaattct tacaaatgga ggtactggtt tttcaaaaag
    19321 agatataacc ccagaagcaa ctaaggaagt gttggaaaaa gaaataccag gtctgagcga
    19381 atatatgaga atgaaatcca cagaaattac taaaaaggcc atactaagta ggggtgtgag
    19441 tggaataaga aataattcta taataataaa tttacctgga agtcctaaag gtgcagttga
    19501 aaatctaagt tttataattg atgttttaga ccatggcata gaagttttaa gaggagaagc
    19561 tacagaatgt gccactaaaa aaagtgaata gtatataatc tagatagata aaaaatagac
    19621 aaagaggtag ataaagtatg attgataagt atggaagaaa aatagactac cttcgtattt
    19681 cacttacaga taagtgtaat ttaaggtgtg tctattgtat gccaccagat gtaaaatttg
    19741 ataaaaatta tataaatgaa aatctaagct ttgaagacta caagtttatt ataaaggcta
    19801 tggctgaaca aggtataaca aaaattagat ttactggagg agaacctctt ttatatccta
    19861 aattaaatga gcttataaaa tttacaaaag aagaatgtgg aataaataac ataggaatga
    19921 ctactaatgc tattgggctt tctgatagaa tttttgagct tgaaaaatgt ggcttaaata
    19981 aagttaatat aagtttagat tctttgaagg aatataagta taaatccgta acaagaggag
    20041 gaaacttaaa agatgtctta aaatcgattg aatcttgttt aaacttaggt ataaaagtaa
    20101 agttaaattg cgttgctata agtgggttta atgatgatga gcttaaagat tttatgtttt
    20161 taacagttaa atcaccaatt gatgtaagat ttatagagct tatgccatta ggtgaggcta
    20221 atagagttta taagaagggt tattttaatg taaaagaaat gattgaaaaa atatatggtt
    20281 tatataaagt caaaaatgat gaaaaaagta cagcagaata ttatatgttt aaaggagcta
    20341 aaggaagaat aggaattata actccattaa gctgttcatt ttgtaacact tgtaatcgta
    20401 ttagacttac ttctagtggt gcaataaaat tatgtctaca ttcaaaagaa gaaatagata
    20461 taaaaccatt tttacataaa ccattgttat ttagagaatc tatgaaagat ataataaaac
    20521 aaaaaccaga aaaacatcat ttaatagaaa ataaatgtag tgatacaaat aggcgtatgt
    20581 atgaaattgg aggataagta atgttaacac atataaatga aaaagggtat gccaagatgg
    20641 tggacataag tgaaaaagat gatactaaaa gaacggccgt agctacagct acaattactc
    20701 ttagtaagga agcattagaa aaagtaaaaa atggtcagat aaaaaaagga gacgtattgt
    20761 ctgtagctca agttgcaggt ataatgggag ctaaaaatac atcttcaaac ataccaatgt
    20821 gccatcagat taaattagtt ggatgtgatt tagaatttga tgtagatgat tatatgtctg
    20881 ctattaagat atacgcaacc tgtaaatctc ttgggaaaac tggagtagaa atggaagctt
    20941 taaatgcatg ttctttagca gcacttacaa tttatgatat gtgtaaagca gttgataaaa
    21001 aaatggttat aagtaatatc catcttgtgg agaagactgg tggaaaatca ggagatttta
    21061 agttcgagta aattaataat atattgggtt aaaatttgga ggaaatatgg cgaaggtagt
    21121 gtcaataaat ataagtgaga aaaagggtgt aattaaagtt cctgtaccat gtgcagagct
    21181 aaaaataaat catgggatag ttggagatgc acatgcagga aactggcatc gtcagataag
    21241 cttacttgga aaagaaagca tagataaaat gaaaatgaaa ggctttgacc aattaaaata
    21301 tggagatttt gctgaaaatg taactacaga agggatagaa gttttttccc tgcctgttgg
    21361 tacaaagtta agaatagggc aatgtgaagt tgaagtaact caaataggga aaaaatgtca
    21421 caatggatgt gaaattaaga aattgacagg tgactgtgtg atgccacgag aaggaatttt
    21481 tgtaaaggtt ctaaaagaag gaattataaa tgtgaatgat tcaatagatg taatctaatg
    21541 aagtcactat taaaatataa tta
//
LOCUS       DAMKPG010000067        21491 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMKPG010000067 DAMKPG010000000
VERSION     DAMKPG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.070"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            50..355
                     /locus_tag="PC621_002537"
     CDS             50..355
                     /locus_tag="PC621_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109684.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            763..1452
                     /locus_tag="PC621_002538"
     CDS             763..1452
                     /locus_tag="PC621_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109685.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            1452..2369
                     /locus_tag="PC621_002539"
     CDS             1452..2369
                     /locus_tag="PC621_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4109686.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            2442..3368
                     /locus_tag="PC621_002540"
     CDS             2442..3368
                     /locus_tag="PC621_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109687.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            3365..4045
                     /locus_tag="PC621_002541"
     CDS             3365..4045
                     /locus_tag="PC621_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109688.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            4490..5191
                     /locus_tag="PC621_002542"
     CDS             4490..5191
                     /locus_tag="PC621_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF4109689.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            6095..6745
                     /locus_tag="PC621_002543"
     CDS             6095..6745
                     /locus_tag="PC621_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4109690.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            7023..9089
                     /locus_tag="PC621_002544"
     CDS             7023..9089
                     /locus_tag="PC621_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF4109691.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            9106..11061
                     /locus_tag="PC621_002545"
     CDS             9106..11061
                     /locus_tag="PC621_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF4109692.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            11077..12267
                     /locus_tag="PC621_002546"
     CDS             11077..12267
                     /locus_tag="PC621_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF4109693.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            13401..13775
                     /locus_tag="PC621_002547"
     CDS             13401..13775
                     /locus_tag="PC621_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4109694.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            13777..14634
                     /locus_tag="PC621_002548"
     CDS             13777..14634
                     /locus_tag="PC621_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109695.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            14637..15275
                     /locus_tag="PC621_002549"
     CDS             14637..15275
                     /locus_tag="PC621_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF4109696.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            16249..16983
                     /locus_tag="PC621_002550"
     CDS             16249..16983
                     /locus_tag="PC621_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4109697.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
     gene            18125..19381
                     /locus_tag="PC621_002551"
     CDS             18125..19381
                     /locus_tag="PC621_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF4109698.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            19444..20637
                     /locus_tag="PC621_002552"
     CDS             19444..20637
                     /locus_tag="PC621_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF4109699.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            20844..21224
                     /locus_tag="PC621_002553"
     CDS             20844..21224
                     /locus_tag="PC621_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4109700.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         8029 a         2341 c         3658 g         7463 t
ORIGIN      
        1 aataaatatt tttatagtag tagttaatta ttaataagga ggggtcaaaa tgcctagcat
       61 gattatggaa gttgtaggag tactatttat agctattgga ttaatcttga cattcattga
      121 aataaaagga aatttccctt ctttttatgg ttttggtcaa aattctatag ggaaaaatgc
      181 tgtaggtatt atagcagcag gaattgcttt aattttcttt agaaatggca tagatagcat
      241 agattctata gctacttatg gtgtattttt agcaatagaa cttgttgtaa tagttgcata
      301 tttcttaata agaaaaaaat ttactaaaga agataaacat ttaagaacta attaactaaa
      361 ttatctacta taaaattgga aaattaaaaa caaataaaag tttgttttta attatttaaa
      421 atcccaattt gattattttt aaagaaacta tattctgtat aaaactagaa tgtggtttct
      481 tttttatgta taaaatattt tttagcaaaa gttaaaaggt gttttataat ataaaatagt
      541 taattgtaga tgttaataaa agaaaattat gtatatttca gataaagata agagagtata
      601 ctatatttta attatttttt caataaaact tattgaaaag aactttaact tagaagagta
      661 tcaccaaatt gcagatacaa tatcataagc agttatttat tgacaaccct ttttaataaa
      721 tatatcattt gaagtatact gacgaaaagg agttgaataa taatggacat cttattggtt
      781 gaagatgata agaatattcg agaaatggta tacgattttc tgattagtga gaattatttt
      841 gttaaaactg ccgttgacgg aaatacagct attgaaatat ttaaaaatta ttcatttgat
      901 attattttac tggacttgat gttaccaaag tctagtggtt ttgaagtatt aaaatatata
      961 cgaagtgtga gtgttgttcc tgttattatt actactgcga aagatagtga cacagataaa
     1021 atgctagggc ttaatcttgg tgcagatgat tatattacaa agcctttttc aatcgtagaa
     1081 ttgcttgcaa gaattaaagc taatattaga cgtgcaacgg tgtatagcag ttcaagaaac
     1141 tctgaagata tggttattca gtataaagat ttgattataa acatatcaaa atatgtagtt
     1201 tcaaagaatg gaattgactt aaacttaaca catacagaat ttgaaattgt taaattacta
     1261 gcgactaatc aaggacgtgc tttttcaaag gaacaattat acaacactat ttggaaagaa
     1321 ccttattatg gaaatgaaaa tgtgctaaat actcatatta acaggttaag aaataaattg
     1381 tctgatggta tagaaaaaaa taattatatt aaaactcttt ggggaattgg ctacaaaatg
     1441 gaggaagaat aatggagtta tttttagttg taatttcgat tgtactattt gctttctact
     1501 taaaagaacg aaataagtca aagaaaatgg aaagtgatta taagtatatc aattcaagac
     1561 ttcgtgatat aacaaatgat gatgaaatta attatatttt agtaccatct gatatttcga
     1621 ttgtaaaaga aacagcagta gatataaata aacttcttga aaagttttac agtaggcaaa
     1681 ttaattataa tagaagtgaa aaagcgatat tgcaaatatt tacaaacata tcacacgatt
     1741 tacgaactcc catcacagta ttaaagggtt atattgaaat gctatatctt caaagtcaaa
     1801 aagaagaatt atcttcttca atgagagcaa ctttggaaaa aatgcaaaca aattcaaatg
     1861 agcttgttaa ttcaataaat aacctgttta atatggcaaa aatccaatct ggagatatga
     1921 tattaaatct tcaaaaatcc aatctaacac aattatgtca tgagattatt ttagagtttt
     1981 atgatttact agataaagaa aactttaatg ttgaagttaa tattgaagat agacctatat
     2041 atgcaaatgt tgacacagaa gcattacaac gtatactcaa aaatttaatt gataatgcta
     2101 taaaatatgg ttcagatggt agatttttag ggatttcact atatgaaaag gaaaaacatg
     2161 tttttataga tattgaggat aaaggttcag gtatttctga aactgaaaaa gagcatattt
     2221 tcacaagagc ttatactgct gacagaaaaa aaggcaatgg tttaggtttg gctatttctc
     2281 aagggcttgc aaactctatg ggaggttcaa tttatgtaaa tagtagacct aatgtaaaaa
     2341 cagtttttac ggtaaaattg aaaagttaga aaaaagttag atttaaaaga gagaaaagat
     2401 aattttattt tgtatactgt ttttagaaag gagggagtat gatgaactat atcatagaga
     2461 cccatcaatt aaagaaaata tataaagata aagctgttgt taatgatgtt aatattcatg
     2521 tgaaaaaggg agaaatttat ggatttgttg gaccaaatgg agcaggtaaa agtacagtaa
     2581 tgaaaatgtt gctaaatctc gttaagccaa attctggaga aatagttatg ttcagtaaga
     2641 aagtggcaga aactgatttt gaaatattaa aaaagattgg tactattatt gaaaatcctt
     2701 atttctatga gaatttgact gctaaacaga atcttgattt acactgtgaa tatatgggtt
     2761 attataacaa agaacatata tcagaggtat tggaatgtgt cggattgtca aagcaatcaa
     2821 acaagaaagt ttcaaaatat tctttaggga tgaaacaacg tcttgcaata gctagagcaa
     2881 ttcttactaa accagaatta ttgattcttg atgaacctat aaatgcactc gacccagagg
     2941 ggataagaga aatgcgagaa ttattccgta agttaaatac agattatgga ataactatat
     3001 ttatttctag tcatattctt tcagaggtag aacaaattgc ggacactatt ggaattattc
     3061 aagatggtaa acttattaaa gaaatttcta tgtcagatat acacaaatat caaacagatt
     3121 atgttgaagt agatgtagat aatgtggaac ttgctggata cttgttagaa aaagaatttg
     3181 gaataaaaga ctttaaaatt acttcggaaa gttgtattga aatatatgat ttacgaaaag
     3241 atgtaaaaga aatatccaaa atatttatca aaaatcaaat tggtataaat agcattggac
     3301 gtaaacaaag ttctttagaa gattacttct tccaaactac aggtacagga ggtatagaaa
     3361 cactatgata catttaatga aactagaatt aaaaaaagtt gggctaaaaa gatatttttt
     3421 attttcaata ctggggattt tgttatgtat gttttttgtc tttactggat tgaatgataa
     3481 atctacttca atatatgatt atgatgttac atttaggaca atcagtttag ttttctgttt
     3541 ttactatatt atattatttg ctgtgttgaa tgtggcatat ataataaatg aatatacaaa
     3601 taaaacaatc ttgattttat tctcttatcc acttgaccgt aaaaaactta ttttatcaaa
     3661 gttacttttg attacgactc ttatgatatc atcaatgata gttggatata tctgttgttt
     3721 tacattcgtt gttgtgttgg ataagtattt taatttagtt attggagatt ttcatatttc
     3781 aattttatct tattggatac caggagcaat taaaagtatt attgtatttt gctcattagg
     3841 tatatggaca ttcgttgttg gaatgataag aaaatcaata cctgttaccc ttgtcagttc
     3901 aattattttt atcaatttaa gacaatttat tttagctggt acaaatacaa ctcaagacag
     3961 tctgatttct gtttttatta tagttggaat aactgtgatt ggtatatatt atatactaaa
     4021 tcataaagtt actgaaattg actagatact gtggatagga aggtagttat taattgtatc
     4081 ttcttgggat aataatgatt tgctattata tcagctatta ttaatattat tagagtttta
     4141 ataatattaa taatagctat atattttttc ttcttattta taaatttaat gatttatagt
     4201 cataaaatga actctaagaa gagataaaaa tgcgttttat atgtttttag attaatatga
     4261 aatatcacaa tgaaatgttg tacaaagtga tatataataa ttatatgaac tataaaattg
     4321 actaaactta ttgactaata aattagatga aaagttgaga cttactgaaa ataaatacaa
     4381 tgaaatatat actacaagta ttaaattaaa tctgaacttt acatactatt agaataagta
     4441 ttataagatt ataatgattt aaaaatatta aaataattga ggtgataaca tgaacaattc
     4501 ctttttagat aaattgattg agacaaaaga acttaaaaat tcactttata atgtgttaaa
     4561 acacaatttt ctttatcatt ctaataagat agagggcagt acttttacta ctgaagcatt
     4621 agcactttta ctcgataaga atgttgttac aggaagacat actttagatg atgtacaaga
     4681 aactgttaat tctagttatg tatttgatac agtaattgac tcactgaaag agaaaataac
     4741 acataatttc ttaaggaatc tacattcaag tttaatcttc aatacaaccc tacattccag
     4801 agggatggct ggtatatata aaactatacc caatatgata ctaggaactg atgtaagcat
     4861 agcacaacca tttgaagttg agccaaagct tgatgaatta attgaatggt actatagtca
     4921 gagtgaagtg tctattaaag ttatagctga atttcattat agatttgaat tgatacatcc
     4981 atttcaagat ggtaatggtc gcataggtag atttgttatg ctaaaacaga tgttagaaaa
     5041 taatcttcct ataaaaattg tatcttggga tagtgaggac ttgtatagaa actccttaaa
     5101 ttcttgtagt ttaggtaatt atgtcccttt aattgaatat ttatcaagtt tagaagattt
     5161 tagagaagta tataaaatgt tatggaaata attgtttgta gcaaaaaaag tttttatata
     5221 aatatatttt taactatgct atttaacgtt gatttcttag ttattcttag cttggactat
     5281 acccactatt ttaactatat aaaatagtgg atatagttta actatctctt tataactatt
     5341 tttatgtatc ttagttgttt cttaatattg ggacagttca taaaactaaa agtgattttt
     5401 aattttatca tttataattt accattacat cagaaaaatg cttatcatta gaattttcat
     5461 aaaaggtacc tattttcttc aattaactaa atcaattcta ttagtcttaa tctttcgtat
     5521 ttcaaattga taatacaaga aaataaaaaa taaaaaattt taaatttttt atttattgcg
     5581 aaaaaaataa aaaggtgcta tactttttgt aacatattat tcaaattatt aagagatagt
     5641 aaatatttat agtttaattt gtagtacact agcatgattt gcagaacaat gaattaatct
     5701 atagtataat agtttaattt gaagtacaac gatttaatct atagtacact agcatgattt
     5761 gaagtataac gatttaatct atagtacaat aacttgattt gcaatacaat aatttaatct
     5821 atagtataat agcatgattt gaagtataac gatttaatct atagtacaat aacttgattt
     5881 gaagtacaac gatttaatct atagtacaat aacttgattt gcaatacaat gatttaatct
     5941 atagtataat agcatgattt acagtacaat aatttaaatt gtagtacaaa agtactatca
     6001 aataagaatt tctactttaa aaatattact aaaagtaatt aaaatccaat aattcaatat
     6061 aatatggcaa aagaatagaa ataaggtgag agtagtgaaa gtaaagtcaa atttagatga
     6121 aattttatat cataagatta ttgaaagtct tattcgtggt gaatactctg taggtcaaaa
     6181 aatattacta aatgaccttt gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc
     6241 agtaaaaatg ctaaataaag atggagtgct tactattatg acaaatggta aagtgtacgt
     6301 accagagtat gaatatgata tggtaaaaca agtttgtgaa acgagaacac tgattgaaac
     6361 atatgcttta gaaaaaatga tgcaagaaga ggaagaggtt tttcaacaaa agctagatgt
     6421 aataaaaaga tactctgata aatgcgagga gtactatcgt caagaaaaat ctgtagaact
     6481 tgcactagca gatttggaat tacataaagc cattgtagaa ggggcaaata atgagatttt
     6541 aaatgatgta tatgttggta tacaaggaag atttatagta gtgaactatt taattcgacc
     6601 attaaaaaat cgtaattatg aaggaacagt acaagaccat tttgaaatat taaaattcat
     6661 tgaagacaga gatacaaaaa aagcagtaga taaactaaga aatcatattc aaggaactat
     6721 taaaagattc tgtgaagatg aataataagt tttgaagtta ctatatactt atctattaag
     6781 ttgctaaatg tagtctaagt ataatctaat tatagtttaa acataatcta aatataatct
     6841 aaatatagtg taaataagtc taagtatgaa ttaaatatag attaagtatg aattaagcat
     6901 ggattaaata tactttaaat tacaaatatt acaaggaagc cagaaggctt cctaaaaaaa
     6961 taacctttat aaaatttttt attttaaatt taaaattttt tattttaaat ttaaaataaa
     7021 aaatgaaaga ggtggtagag agtttgaaaa aaattactaa agaaaggata gaaaatacta
     7081 tgaaaaagct atttacgagc gaaacatttt tggaatatca atttataagt gattgccaaa
     7141 tatcaccaga tggaggatat acagcattta ttgtaaaaaa agctgatatc aaagagaatg
     7201 gttacacaag ccaagtttat atattaaaca ataaaactgg cgaattaaaa caaataacgt
     7261 ctataaactc tgttggagca tatgtctggg aagatgaaaa tactatcttg ttccctgctt
     7321 tgcgaaatga aaaagtaaaa gaagctgtca aaaacggtaa gcaatgcatg agttactatg
     7381 ctttatcttt aaatggtgga gaagcagaag aactttttag actccctatc aaaggtggta
     7441 aattgaatcc tataggaaaa ggtttgtatg cagttattga ttcttatgac aatgaccgac
     7501 ctattgtaga gggattgcca gaagaagaac aacagaaaaa aataaatgct tataacaaaa
     7561 gacattatga acatttcaaa gaaattcctt atgctgtcaa tggggaggga tatattagca
     7621 gaaaaagaaa acgcctatat atatacgaca gtaatataaa taagttgaag gcaattacag
     7681 cacctatgtt taatgttgta ggtatgaaaa taagtgatgg aaaaatctta tatatagggc
     7741 aagagtttaa agatgtaaaa ggtttgaaaa atggggtcta tgtatttgat acaaacacaa
     7801 acacaaatat ctgtatatta gaaaaagata agtatattat aaaaggtttt gaattatatc
     7861 aaaatcaagt gatactaaac ctaactgatg ctttatctta tggaaatgga gaaaatggag
     7921 atttttatac tattgatatt gatacgaaag aaatgaaact tctttcaggg catcaacatc
     7981 attgtattgg aaatacagta acatctgatg ttaaaatggg tgctggtcag acaacaaagg
     8041 tagatggaga atatatttat tacacttcta cagtagatat ggactgcatt attgaaagaa
     8101 tacatattcc aacaggaaaa caggaaaaag taacacagac aggttcagtt gattttatag
     8161 atgtaaaaga tggcaatatt gtatgtgtgg gttgtttagg aaatggatta ccagaagttt
     8221 atacagtaga gaatggaaca ttgtgtaaga aaacacattt aaatgaccat attttagagg
     8281 aatataaaat ttctgtgcca gaatacatag agtcaaaagg aagttctaaa tgggaaatac
     8341 aaggatacgt atataaacca gtagattatg aggtggggaa aaaatatcca gctgtattgg
     8401 cgattcatgg aggaccaaga ttgacatatg gaccatattt tatgcatgag atacaggtat
     8461 ttacaagtgc aggttacttt gtattcttct gtaacccaag ggggtctgaa ggtcgtggca
     8521 atgcttttgc agatattagg aaacagtttg gagatattga ttatatagat tttatggaat
     8581 ttacagatac tgtacttgaa aaatatacag acattgataa aacaaaattg gctgttgaag
     8641 gtggaagtta tggtggattt atgacgaatt ggataattgg tcatacaaac cgttttgctg
     8701 ttgcttgtgc tcagcgttct attgcaaact ggagtagcat ggagggtaca acagacattg
     8761 gttattattt ctgcaaagga caaacaggtg cttctcatat ggaaaaccac gagcttcaat
     8821 ggaaacagtc acctcttgct tatgctgata aatgtgttac accgacttta tttttacatg
     8881 gtgaaaaaga ctatagatgc tatatgcaag aagcattcca gatgttttca gcattgaaaa
     8941 ttcatgggtg tcctacaaaa ctttgtctat ttgaagggga aaaccatgaa ctgtctcgtt
     9001 ctggtagacc aaaacaaaag ctacaaagat tagttgaaat gctagattgg ttttctgtgt
     9061 acataaaaaa agataacata ggacagtaga aaggagaata taaaaatgga aaaaataaag
     9121 attgaaagtt cagatatttt tgaatatata ttccctcatg atatttcttg ttcacctgat
     9181 ggaaagcata ttgcttatat tatatcaaat atcaacgagg aaaaagactg ctatgaacat
     9241 gacttatatg tgatggacat caaaacagaa aaacaaatac atatgactca aacaaaagat
     9301 gtaacatcat ttagttggat ttctaataca gaactacttt ttacatctaa aagaaataag
     9361 ccaaaagcag gtacaacaga tttttacact atttctatag aaggaggaga agcaaaagaa
     9421 gcattttcta ttccaaaagc ttgtagcgta cctgtatcac ttggaaataa attgtggtta
     9481 ttgacaacaa aaaatccaac ggattctaaa aaatcagaac cagatagggc agtagagggt
     9541 gtagattatt ggacatttac agataaacct tttataagag atagagaaaa tttttctcaa
     9601 agaagacgtg taacattaga attatatcaa gaaggtgaaa atataacaaa agccattaca
     9661 ccaaaatttt gtgaagtagc aggtatagac gtttcttctg ataaaaacag aattttatat
     9721 actggtcaga tttatgaaga ttgtgcaact cctttttctg gactttggga gtatcatata
     9781 gacagtggtg aaaccaaaga acttgtacca caaggaaagt accaaataag tttggcaaaa
     9841 tatattggca aagataaagt aatgttgcaa gcttcaacat tagaacgttc tatcacacag
     9901 aatcatgata tttttatact tgatttgagt actagtgaaa taaatatgat tgctagtcca
     9961 gatggtatgt atgctacatt actggatgta gatgcagtat atggtggtgg acgtagtaat
    10021 aaagttatag gagataaatt tattggtgca agaatttgca gaactatgac agaatttaat
    10081 gagtttgata caaagacagg taatatacgt atcatcacaa aagtagatgc ttttacatct
    10141 tttgatattt acgacaacac catgtacaca gtaatgttga aagattatga gttagcagaa
    10201 atatatagca ttgatatgac tacaggtaca atgaaaaaaa tgactgcttt ttctaaacca
    10261 tatttagata ctcataaggt ttcattacct gaaaaattga cgtttgtagc aaaaaataaa
    10321 gaagaagttg atggttttgt tataccacct atagatgcta aagaaggcga aaaatatcct
    10381 gctgtattgt tcattcatgg tggacctaaa tgggcatatg gctatatgtt tacacacttg
    10441 aagcagtgtg taacatcaaa gggaatgtat gtgatttatt gtaaccctca tggtggtgac
    10501 ggttatggtg aaaaattctt agaaatggta gaaagatggg ggtatgttga ctatgagcat
    10561 cttatggaat ttgtagatac ttgtattgaa aaatatcctg ggattgatgc agaccgctta
    10621 ggggttgcag ggggaagtta cggtggttat atgacaaact ggataattgg ccatacagac
    10681 cgtttcaaag cagcagtttc tcaaagaggt attagtaact tgattacagc ttctttaatt
    10741 attgactttg gggatagaat tatgaagcag acttgtggag ataagacgcc ttggaatcat
    10801 gaggaggttc tttggaatca ttcacctatt aaatatgtga aaaatgttaa gacaccaaca
    10861 ttgttcttac atagtgatag ggattataga tgttttatgg gggatacttt ccaaatgttt
    10921 acagcattaa aacagcttgg tgtagataca gaaatgtatt tgttccatgg agatacacat
    10981 ggattgtcta gaaatggaag accatctaat agaattgcac gagcaaatgc tattgtagat
    11041 tggtttgaaa gatatttata ataagggggt tattctatga gtgatatgtt attatggttt
    11101 agtgtcgtat tagttttatt tgctattggg gatttaattg caagcaaaac aaaagcaaaa
    11161 gtatcagctg tatttgttac actactttta tttttgattc tatttgtcac aaaagcaata
    11221 ccagctgata ttattgaaaa agcaggtatg accgcagcag ctagttggag tgttccaatg
    11281 attatgttta gtatgggaac aatgttgaat gttaaacaat ttatagacga atggagaaca
    11341 gtattgacag catggcttgg aattgtagca gttatagtct gtgtctcatt atgtattcct
    11401 ttgtttggaa aatcaacagt attgacaagc ataccagtta taaatggagc tttacctgct
    11461 actactatta tgacacaagc agcactagaa aaaggactga cacttgcagc agctacagca
    11521 acagttgtat ttgcaattca gaagtttatt ggtacaccaa tagcttcaag agcagcgtta
    11581 caagaggcaa accgtctttt agtagaatat catgaagcaa aatcaaaagg cattgattta
    11641 gcaaatgttg atactgataa gaaagaagca gagaatacag gcacaaaagt aaaacaagct
    11701 ttctgtgaaa aatacgataa atattattct acaaatgttt gtatattttt tatagcacta
    11761 ttctcttatc tagggtatga actttcagag attattcatg taaattattc tattgtatgt
    11821 cttgttgttg gtgttattgt aacacgtatt ggtattgtac caaaagacat tttggaaaaa
    11881 ggaaaaatca agggatttat taacatggtt gtgtttgcag ctgttattcc ttctttagca
    11941 aaagtttctt tgacagattt aattagtctt tttgtaccaa ttgtgggtat gtttgcagct
    12001 tctatcattg gtatattcct tatgatgaag gtgttaccag gttggaaaat tatcggttct
    12061 aaacctcttg cttttggagt agggttttgt cagatgttgg gattcccaac tacatattta
    12121 atttcaaatg aagtgtgtaa tgctgttgga gaaacggaag aagaaagagc atatttgatg
    12181 tctaagatta tgcctaagct ggtagttggt ggtatggctt gtatgatttc tattgtagta
    12241 gctggtctta tggtaccaat gttgtagata ggtttcatag ttaaaaagtg gtgttaattt
    12301 ataggagaag ctaaaaagta gtattaattt ataaaataat tttatattag ttaatttgtt
    12361 ataagaatat tttcttttta tgattataat tttgatttta taagagagca gattttttat
    12421 ttgctatgct aatagttata atttgttttt atttgctaaa atgcaacttt aatttgattt
    12481 atgtataata tagggtattt aataatattt ttgaagaatt tttataaaaa attaaaaaga
    12541 ggagaaaaaa gggagtgcca caaagtgaac tttttagttc taaggcactc ttttttgtac
    12601 gaaatcgaat atattttcaa cgttttaaaa gtgagaaaga gacttatctc tagtttggga
    12661 tagccaaatt taattattaa aattattttt tgttttattt atcaaagagt attcgtgttt
    12721 tagaagttag tattttttat tttatatatc caaggttttc cgtttctaat gttttctttt
    12781 ttaaaataat tagggtattc aattatagta aaaggtggat ggtattttgg aatgatatta
    12841 tttaaagcac agtattgaat gttgaaatcc tcacatttta gagtattatc ttttgtaatc
    12901 aaaaaaatct tctatactta tagcaaggtt acttctgcaa atattccatg catcataata
    12961 tccaaattta aattttttta atatagagtt tacctctagt ctacactctt gaataactct
    13021 ttcctctatc caaattctta taaactcatc ccatttatta ctatctattt attactatct
    13081 aaatttttta gtatgtttgt aattggaaat ggatttattc ctttatgttt tttataaata
    13141 gtttataact taatttatta ttaaacaaat cattcataca gaattacttt ttcttttaga
    13201 tattcaattc ttacttttaa taaatattta atacattcta tcttatgtgg ttttaaaaag
    13261 tgtttatggc tatcaataat ctctaatatt tcattcatat ttattttgtc atagtaatat
    13321 tttacatctt ttatattgac aaaaaagtgc tctaagtgta tagtgtgtat ataataacat
    13381 cacactggag gtgataaaaa atgaaagtgc tcatttctaa cacatcagat aagccattat
    13441 accaacaaat taaagagcaa attaaagaag caatatttac tgaagaactg aaagaagggg
    13501 atgcaatgcc ctcaataaga aattttgcta atgatttaaa agttagtatt ttgactatta
    13561 gacgagtgta tgaagaactt gaaaatgaag gttttcttac acgccaagtt ggaataggaa
    13621 catttgtttc aactggaaat ttagaaatac taagagactc aaagaggcgt atagttgaac
    13681 aaaaaatggc agatatgatt aaggatgcaa aagcactagg aattacacag atagagttac
    13741 aagaaatgat gggtattcta tatgaggagg attaatatgg aaaatatttt agaagtttat
    13801 aacttaaata agacttatac caatttttca ttatctaata taagctttgt attaccagaa
    13861 gattgtataa ctggatttat aggtgttaat ggagcaggaa aaacaacaac aattcgttcg
    13921 atattggatg tcgtaaagcg tgatagtgga aaaataaaaa tctttggtaa agatttcgat
    13981 ggaaatgaga aggaattaaa aaatcgaatt ggagttgtat ttgatgattc atatttctat
    14041 gaggagttgt caatgtcaga aatgaaaagt attattgcac cttcatatga taattggtgt
    14101 gaagaagatt atcaacaaaa tatgaagcgt tttaatctta atccaaaaca aaagattgat
    14161 acactttcaa aaggaatgaa aatgaaattt gctttagcat tagcattatc gcataaagct
    14221 gagtttttaa ttatggatga gcctacaagt ggtttagacc cactaatccg tagtcagata
    14281 cttgacatac taacagaata tatgaagaaa tgtggtcgtg gtgttttctt ttctactcat
    14341 ataacatctg atttagacaa aatagcagat atgattgtta tgattgacaa tggacgtatt
    14401 atattagagc gtgaaaaaga tactctattg gatgaacata gaaaagtaaa aggggatatt
    14461 caatttctaa atgatgaaat caaaaaatta tttttaaatt tagaaattac aggttatggt
    14521 tttataggga ttacctcaaa aattaatgaa gtgcaaaaaa atatgccaga tattttaata
    14581 gagcgtccta caattgaaga tattatgctt gcatatattg aaaggagaga gtaattatgt
    14641 tgattcattt agttaaaaaa gatattctga ttgtaaaaaa atatgtattg ttgatattgt
    14701 ttattgcaat aggaatccca ttatttatat tatggcgttt acctccattt gcacaaatat
    14761 tgggatttgt actatcaatt atattttcag aatttatgct gtgccagtat ctattattaa
    14821 aggagagtca atattcaaaa gcatctgtat tactttgttc aactccttat cctagacgtg
    14881 gcttagttca atctaagtat gttttatttg caatggtatt tatctattgt acattagttt
    14941 actggattga aaatttactt attccacaaa taggaacttt tgacttaaca tatattttga
    15001 ttgttttatt aataagttct attatttatg gagtttatat gcctattcaa tacaaattag
    15061 gatatgaaac aacaaaattc tttttcgtga tgataatcat ggcttcatca tttgtattac
    15121 cattaactat agctcacagc aattttgaaa ttccatttat atatcagtat ccatccatga
    15181 tagtgaatat tgtattgttc cttttaagtt taattatact gttagcttca atgtttattt
    15241 ctattaaaat ttttagtaat aaggagcttg tataaggtaa gggggaattt tatatgccac
    15301 atttgccaga ttgggctgtt ttactttgta tcttacttcc aatagtatat aaagcttgtc
    15361 agatatttaa aagtaaaaaa tagagtacat atttatattg ccatttttat tgagtgaagt
    15421 tagatggtat gaatttaaaa ggttacttga aaataaagtt acttggtgtg ggagagtaac
    15481 atcataatat tatagattat atgtttcttg tgaatcaatt tgtatttttt aatagataat
    15541 atccagtaat tttattttcc attgattatt ttcaattatg agtatgattg tgtgtattgt
    15601 ataccatctt ttaaggtgtt tttaaaaaat tatatcgtca aaatataaat ctaaggaatc
    15661 ctataaataa agcttttcaa ttttagaaaa ctaataaaaa gtaggaagtt aataaaaaga
    15721 agctgtctca aaatagagat aagcttcttt ttcattgctg aaatgacgaa aatataattg
    15781 atttaatata aaaaagagtg ctttatgaac tatttatttt gaacagtaga tacacttgca
    15841 ctcaatttag ttactcctaa gctttatgaa ctaaaagttt attttgaagc ctatttttct
    15901 aaataatcat ctatatacag tattgcgttt cttttattta ttcataattt attttcttat
    15961 taaagttttt ctatattact ttacttttaa ttttatacga attatcaaaa ataaaatagt
    16021 tattagaaaa aaataaaagt aattgcaaag attgcaatta cttacttcta tattaattac
    16081 tataccataa aataacaaaa aaatcaagtc gagtaatcct tcaacttaag tgatatacta
    16141 ggtattaata atttttaatt aataaaatat aactagagga aaatctacat tttctcaatt
    16201 tctttaatta aattgacctc taattaagta attataagga gaaaaattat gaatttagca
    16261 aagggattag atgttttaaa aatttcatca aatgtattcg gtgaagataa agttatgtat
    16321 attcctgtaa tctacacaga agatgatgca acacttattg acacaggtct tccgggtcaa
    16381 ggtgatttaa taattgatgc tcttaataaa agtaacacga gctttgatag gttaaaaaat
    16441 ataataatta ctcatcatga tatagaccat ataggaaaca taaattatct aagagaaaaa
    16501 tctaagaata atatcaaagt atatgcatac aaaagtgaag tcagttatat aacaggagaa
    16561 gaaactcctt ttaaacttta tatgttggaa caaatggtag ataaaattga tgataagatg
    16621 cttagtatgc taaatgttat ggtacttgga tttaaatcat catatactaa agtagatgtg
    16681 tcattagata accatgaaaa attgaatcta ggtgaggaaa tagaagttat acatacagga
    16741 ggtcacacta gaggtcacat atgtctttat ttaaaagagt ctaaagtact tattgctgga
    16801 gatttgttac aggtagaaaa tggagaactt acgcctgttg atgtaatgca cagcaacaaa
    16861 caagagttaa aagatgctat taaaaatata agtaattatg atatagaaac gatagttttt
    16921 agtcatggtg gattatatca aagagacata attggaacat tgaaaaacct aattattgag
    16981 tagtactata tgatttacaa taatttctta aattacttaa aattatatct aaagatgtag
    17041 aatcaaaatt aaatataagt aataagaatt ttgctttaaa attatactta agtgttaagt
    17101 atgatacgta gtattacaag tattttagaa tataattttt tatagtaatt ttaaggttct
    17161 acaatacttc agttgaaaaa tatttaacag catagtataa taggaaagaa aatattttaa
    17221 ataaacttta gtatacaaag tgaagaaaaa tatttaaatt atatataata ttgtagagtg
    17281 aggtaaaata tttaagttat atatcataat acagagtgaa gaaaaatatt tagataatgt
    17341 accataatac aaggtgagta aaaatattta aagcatgtat aataatgcaa agcgaagaaa
    17401 aatatttaaa taatgtacca taatgcagag tgagtaaaaa tatttaaata atgtgttata
    17461 atacaaagcg aataagatta tttaaagcat gtattataat ataaagtgag taaaaatatt
    17521 taaatcatgt atcataatat aagcagagtg aagaaaaata tttaaataat atatcataat
    17581 gtagagtgag gtaaaatatt taagttatgt atcataatac aaagtaaaga aaatcattta
    17641 aattatatat cataaaaata atatgaaata ttgaacactc ataatattgg aggctttaat
    17701 attttaattg ataggttaca ttatttataa cattaaaaat acaatttaca taaaatagac
    17761 atatgaaaaa taaaaaatta tataataatt atgggaatga ggttctccct tggatgataa
    17821 tatcctaaac tgccaatgag ctgatgactt ctatgatatc atagatgcca ccagcttttt
    17881 ttgttgttta aatcgataaa atggaggtga attacttact aaatttttat tttttaaatt
    17941 tgaagaaata tttaattaat agaaaaaaca gtagattata tgttaattat tttattaaga
    18001 atgggttaag agtataccta tatcctatca atttaaaaaa caattatgat agtcattaca
    18061 ttgactacag ttgatgcaat aaaactttca aaaaatctta tgatgcaaat agaaaggaat
    18121 taaaatgata gaaaaattta aaactttaat atggattaaa atgataataa gaattaaaag
    18181 cgtttataaa acatatggtg gactattttt gttaggactc ataggtatac cagttggtgc
    18241 aattataggt cttatagaca ccatatttgg aacggttctt ctaaaagtta cagatattcg
    18301 agaaacatat ccaatgtatc tgataccatt tttagcagta gtaggagttg ttattgctta
    18361 ttgctatttt aaatttggag gaaaaagtag taaaggaatg aatctaatat ttgaggtagg
    18421 gcatggagaa gaagaaatta ttcctttaag attggttcca tttataatat caggtacatg
    18481 gttaacccat ttatttggtg gtagtgcagg acgagaagga gttgctgttc agattggtgc
    18541 aactttttca cattgggtag gtaaaaggtt gcctataaaa aatgcttcta gtatttttct
    18601 agtaactggt atagcagctg gttttgcagg attatttgag acaccaatag cagcaatact
    18661 atttgctatg gaggttttgg tagctggttc actggagtat caatcgcttt ttcctgcgtt
    18721 tactgcttct tttacagcaa gtgcagtatc aaaaacttta gggttagaga aattttcttt
    18781 tgcactttct tctaaagttg tatttgactt accaatattt tggaagctta ttgttttagg
    18841 aattattttt ggaatggttg gtggagcatt tgcatggtgc ctgaaattgt caaaaagaaa
    18901 aataggtaat agattaaaaa atcctatgat aaggattgct attattggag tatgtcttag
    18961 tgtattattc cttttatttt ataaaggaag atattcaggt ttagggacaa atttaattca
    19021 aaatagtttt tatggtggag aaatttattc atttgactgg ctgttaaagt ttatacttac
    19081 aatactgact ttgtcagcag gttttcaagg aggagaggtt acacctttgt tttccattgg
    19141 agctagtctt ggtgtattgt tagcaggatt ttttaatttg ccaattgaat tagttgcagc
    19201 gcttggctat gctagcgtct ttggcagtgc tacaaatact ttttttgctc ctgtatttat
    19261 aggggcagaa gtttttggat acagctattt accttatttc tttgtagtat gcgctatttc
    19321 atatatattt aatatggata aatctattta ttcactacaa aaaatttcaa cgaaacaata
    19381 aataaaacaa cataatgtat gattataaga gaaagaatat tagagaaagg atagggtagg
    19441 aatatgttga caagtttagc tttgattttt ttattaggaa tggcctcggg aggtattttt
    19501 gagaagataa aattacctag cttattggga atgttactta caggaatcat attgggttca
    19561 tatgttttaa atttgattga taattcaata ctagatattt cttctgacct tagaaagatt
    19621 gcattgatta ttatattaac aagggctgga ttatctttag atataaacga tttgaagaaa
    19681 gtgggaagac ctgctgtact tatgtgcttt attccagcta cttttgaaat tatcggaatg
    19741 atagttttag caccaaaatt actaggcgtt tctatattgg aagcagcagt catgggtgca
    19801 gtagtaggag cagtttctcc cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa
    19861 ggttatggta cagaaaaaag tattccacag atgcttttag caggaacttc tatagacgat
    19921 attttcgtaa ttgtgatgtt tacagtattc acaggattgg cacaaggaaa tagtatatca
    19981 gctattagtt ttttacaaat tccagtatca attatattag gtgtaatagt tggagcagtg
    20041 attggaatct gtttagcagt attttttaag aaggttcata tgagagattc tgctaaaggg
    20101 gtgttgcttt taagtatctc atttttaatg ataagtttgg agactgcttt agagggtatt
    20161 gtgccatttt ctggattatt agcagttatg aatattggga ttttccttca gataaaatat
    20221 cgtgtagttg caagaagatt gtctgtaaag tattcaaaac tatgggttgg tgctgaaatt
    20281 ctcttgtttg ttttggtagg ggcaacagta gatataagtt atgcatttaa agcaggaatt
    20341 ggagctgtaa ttttaatatt tggagttctt ttattccgta tggtaggagt attcttctgc
    20401 cttattaaga caaatttgac aataaaagaa agattgttct gtatgattgg ctatattcca
    20461 aaggctacag tacaggcagc aataggaggt gtaccacttg caatggggat agcatctgga
    20521 caacttattt tgacattagc ggttctcgct atacttataa ctgcacctct tggagctttt
    20581 ggaattgatg taacttataa aaaattatta acttctgtag aaggtaagag taagtaagaa
    20641 taaaaataaa gattatttta agtgataaat tatattttgg aaaaaactat attattgagt
    20701 acaatgttag aaaatcattt gagtgattac agaaggtaga agtttatatc ggattttaag
    20761 gcatgtgatg attattagaa ttgattttgt gaaattgtta ttgtaatatt taattatata
    20821 gataaagaag cggagtgata actatgtatt taaaaaaatt atcaaaatta gagttagtta
    20881 ttatgaaatt tatttggaat ttagatataa aaaccaattc atatgagatt attgattata
    20941 tgaaagaaga gcataattta ccagaaaaag tggctttaaa aattttatca aaattatcaa
    21001 agaaaagatt cttatatgtt caagaaacag gcaagtgtat gtattataca gttgcaataa
    21061 aagaaaagac atatttagag tttatatcaa gaaatgtgca aaatcttttg aagaataatt
    21121 ttataaggaa tttattagta tcatttcacg aagaggagct aacagaggaa aagataaagt
    21181 cgttagaaaa ctgggtagta aattgggaag aagcttatat ataaatgtta ttatttttat
    21241 tacaatttag ataattttgg ttttgtaagc tctgcaacta tttttagatg gttgcagagc
    21301 ttatttttat tttttattcc cttaagcaat acttaaaagt aaacgcattt atatttacta
    21361 acttcaatca cttctaatac ttagattact ttttaaaaat attttgatat gtcatctcta
    21421 tagtaatcta gaacttgttt ctattcttta tataaagttt ttatgaatga tatatataat
    21481 attcgttcat t
//
LOCUS       DAMKPG010000068        21487 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMKPG010000068 DAMKPG010000000
VERSION     DAMKPG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.071"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(155..2542)
                     /locus_tag="PC621_002554"
     CDS             complement(155..2542)
                     /locus_tag="PC621_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF4109701.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(2734..3489)
                     /locus_tag="PC621_002555"
     CDS             complement(2734..3489)
                     /locus_tag="PC621_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF4109702.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            complement(3514..4665)
                     /locus_tag="PC621_002556"
     CDS             complement(3514..4665)
                     /locus_tag="PC621_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF4109703.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            complement(4865..5605)
                     /locus_tag="PC621_002557"
     CDS             complement(4865..5605)
                     /locus_tag="PC621_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF4109704.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            complement(5677..6666)
                     /locus_tag="PC621_002558"
     CDS             complement(5677..6666)
                     /locus_tag="PC621_002558"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF4109705.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            6926..8179
                     /locus_tag="PC621_002559"
     CDS             6926..8179
                     /locus_tag="PC621_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF4109706.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            8276..8500
                     /locus_tag="PC621_002560"
     CDS             8276..8500
                     /locus_tag="PC621_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF4109707.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(8716..10023)
                     /gene="grdB"
                     /locus_tag="PC621_002561"
     CDS             complement(8716..10023)
                     /gene="grdB"
                     /locus_tag="PC621_002561"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8974..8976),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF4109708.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(10109..11395)
                     /locus_tag="PC621_002562"
     CDS             complement(10109..11395)
                     /locus_tag="PC621_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF4109709.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(11674..12996)
                     /locus_tag="PC621_002563"
     CDS             complement(11674..12996)
                     /locus_tag="PC621_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF4109710.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            complement(12996..14990)
                     /locus_tag="PC621_002564"
     CDS             complement(12996..14990)
                     /locus_tag="PC621_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF4109711.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            complement(15077..16324)
                     /locus_tag="PC621_002565"
     CDS             complement(15077..16324)
                     /locus_tag="PC621_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF4109712.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            16552..17097
                     /locus_tag="PC621_002566"
     CDS             16552..17097
                     /locus_tag="PC621_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4109713.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            17111..17797
                     /locus_tag="PC621_002567"
     CDS             17111..17797
                     /locus_tag="PC621_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF4109714.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(17921..18541)
                     /locus_tag="PC621_002568"
     CDS             complement(17921..18541)
                     /locus_tag="PC621_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109715.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(18531..19553)
                     /locus_tag="PC621_002569"
     CDS             complement(18531..19553)
                     /locus_tag="PC621_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF4109716.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            complement(19554..20192)
                     /locus_tag="PC621_002570"
     CDS             complement(19554..20192)
                     /locus_tag="PC621_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF4109717.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(20310..21461)
                     /locus_tag="PC621_002571"
     CDS             complement(20310..21461)
                     /locus_tag="PC621_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4109718.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
BASE COUNT         6855 a         3755 c         2258 g         8619 t
ORIGIN      
        1 atgtttttaa acttatacta cataaattta aagctgaaaa caaaacttta caatcattga
       61 ttatcttatt tatcaataat ttaaagtaat tattttcagc ttttattaag ataagttact
      121 aatacacact aatatatgtt aatacttaag tactctattt agcacttttt aattttacta
      181 actctttaaa tgcattttta tttgctcctc caccaacttg agttactttt tctaaagtct
      241 tatttttaag aacttctttt tggctatcag ctaatttatc accaactaaa attactggtg
      301 acccagtttt tgctcctaaa ggaccaactg ccaatccatc tattaaatca ttttgattct
      361 tgattccatt tttaactaca aatgcatttt ttaaagtatc ttttttataa aaattatcta
      421 ttatctttga attagtttcg tctctactac taccagataa tctagttgga ttttttaact
      481 tactttctag attgtttgat agggatgctg ttcctccaat aatataagat ttctctatat
      541 ttaatgaatt tagaaattca tcagctcctt gcaattcgct cttttcattt gcaagtatga
      601 ttggtatgtt attttctgct gcagcagcac ctacactaat tgcatcagct aatcctttca
      661 ctccatttac aactgctatt tgagatacat tttttatacc atttaacttt tgagctaata
      721 ataaacttgt cttgtatcta tcacttcctg atattctctc tatcgatata tttaagcttt
      781 ttaattgttg ttcaatttct ttacttatag aattttctcc gcctattagg tatactttct
      841 tagcacctaa tctctgtatc tcagctttag tttttgaatt taaagcttct ttttgtgtca
      901 ataatattgg tgaatttata gcagatgtaa aaggagttgc tgccaatgca tctactattg
      961 aactgccatt tactaaaact acattttctg aagaattgaa accattttga ctaactttga
     1021 tagcagtttc atatctatca ctaccaatta aagactcatt cgggatactc tcaccagaag
     1081 acttttctgt aacagctttt atagtaaaat tacacggata gaagttacta tctctattta
     1141 tatcaaagaa ttttccagta ttatctatga tataactttc acctaaatta gcttttgctt
     1201 taaatgtatc atatcctggt acatttgact cagaacctat agttgactct ccatttttcc
     1261 cctctatact gactacaatt gcaccctttc cttctggtat attaaaagaa tcaattggaa
     1321 ctgttatgta tcctgaatat tgggcagtac cttcttttaa ttttttcata ttgttttctt
     1381 gaggtatacc atttactact ggagcataag atacttcata cttagctcct atactttcag
     1441 ttaagaacat tacagatttt agagtttcat cgcctctatt gaaatcaaat acatttgcag
     1501 ctgttatctt ttttgaaatt aaaggtacaa tacttgcata gtcatgttga tacatcttct
     1561 catcatcatt tggtttttta ccacctttga ttgaaaaatt atctatatca gacataagtg
     1621 tcttgtcttc ataagatatc cagaaatatc caccctcaga attataatct ccccagctat
     1681 tttttattaa ccaagcacca ttttttgatg gtctaatatt tgcattaaaa ttatctttag
     1741 aatagttatc atcccaaccc acaattgaaa tagcatgatt tgtattaaaa ctttttgttg
     1801 gacaataata tgaattttgg tcatcacttt gatatttaat atcttcataa taaccagatg
     1861 ttactgctcc atactgtaat atagcatttt ttgtagaatt tatgtcatta cttacacaca
     1921 cagcatctgt cacattaaaa acagtaggtg ctgtattcat attagttggt ttttttgctc
     1981 cttgtgcttt agatactcta ccattgtatg ggatatcttt ttctaattta ggacctgacc
     2041 aagatgttaa atatcccata gcttcataat ttaaagcacc atccatatca tttacagacc
     2101 atccatattc accatctgaa gcccaccatc ttaagtgttc ttctgataaa tcgtagtctc
     2161 cataattatt taacttcaag aaactttcca aggttgccat tgcagcaaaa tcccagcata
     2221 ttcctaaatc ctcttgattt tttactgatg tcataagacc taattttcta gggtcataag
     2281 atgttggagc tgttcttcct ttactttgaa tttgagtacc ttctaactga tatggatttg
     2341 gtataattcc attatatttt tctgtacgtc catttttagc atcatttaga tattgtttat
     2401 aagtatcact gtattctgct ttttttatct gattcaatgt attgctattg tcagctgaca
     2461 caggaattgt tgacattact aaaatagata ttaaaaacag tgcaacaaac cttgctactt
     2521 tttttgaagt aaattttttc acgctttctc ccccttattt tgagctaatt tttaaaattc
     2581 taatgttata tatgactaaa tatcaagtat ttacaaatat acaatcattt aaatcaatta
     2641 taccaaagtt ttcctttata aataaacaaa ataaaataaa aaactgaact tatttatgat
     2701 aaattcagct tttcattttc tttttatatg tttttatcta aatcctatta cagcagctcc
     2761 taaagctcct attatttgag tatcttctgc aataaaaatt tttttaccca gtatttcttc
     2821 tatcattttt actagttctt tactttttgc aagccctcca gtaaatgcaa cttcatctat
     2881 tacctcacct cttgctaaca ttgaggtgga tttatttgca atagatttaa gtatccctgc
     2941 tgctacctct ccagtagaaa tgttttgagc tagaagactt acaatttcag actcagcaaa
     3001 aactgtacac atactagata tattaactgg ttcatatcct ttagctaatg tatctatact
     3061 ttcaatatcg cttcctaaaa ggttagaagt aatctctaaa aatcgaccag ttcctgcagc
     3121 acacttatca ttcattataa agttaaatac atttccatct ctatctaaat ttataacttt
     3181 actatcttga ccacctacat ctaaaatagt ccttatgttc ttgtttaaaa agtaaatacc
     3241 tctagtatga caagttattt ctgttacttt cttatcagca aaatccatta ctccccttcc
     3301 ataacctgta cctaccacct tttttatatc ttttttgtca atttcactac ttagcaactc
     3361 atagacctgt ttagatgtat ttttagggct ccatccagtt ggtataatta ttttcttaat
     3421 aattttttca ccatcaaata gcacaccctt tgttgccact gaccctgagt caactcctat
     3481 actaaacata gttcctccta cttttattta ttcctacaac atctctacaa acgcttcaaa
     3541 tctagttctt aattgttcag tatctgattt tgaataatca gtttcaagag acataaatgg
     3601 cttatttttt tcttttgtaa caaattctct tattctataa ctttctatat taaatgtatg
     3661 acatgcttgt aaaactacat ctattacagc atctacatta tattcatcta ttattctatc
     3721 taataattca atacgattgt cattattcat catacaagaa caacctatat ttatatattt
     3781 ttgtgttaaa gcatcatata catcttctat ttcttcatca accaataaat cgtttgatct
     3841 aatagctcca caaagttcat atgcaacgac tgatgcccct gcatcttcta aagttttaac
     3901 tattttttcg cttattccac caataggaga acctgttatc aatattcttg gcttatcttt
     3961 ttgaacagga cattcacctt tttcatatct ttctttcata tcatctataa cttttcttaa
     4021 agaatgttta agttctgcac ggtcaaattt aaaacctgct tgatataaaa cattatgtaa
     4081 ctcaaggcct gtaagtgcag atggttctaa tttaccaagt gcataaaact cttttaaaag
     4141 ttctctttct tcatttttat cttttatagc agttcttata tcttcttcag ttatagttac
     4201 acctaaagat ttctcaacct cttcttttaa tcttatcatt tcatctttcc aaagtttata
     4261 ggcaaattct ccttttgaag tatttggcaa atgcattaca tatgttggct taacttttgc
     4321 aagttcttca tacatttttt tcttaccatc acaagtggtt tctcctatca acaaatcaga
     4381 aaaatagaaa taaggacagg tgtctgttat tgcatgtcca tagcttgatt taattaaagg
     4441 acaaagattt cttggaagca ccctttctgc atcaggtatt ggctcttcac taacaccgca
     4501 tacacctact acagttgctc cagctgccat tggaatttca actggagtaa atgtacagaa
     4561 aactccaacc atctttttcc cactttcctt aagttccttt gcatttataa atcccttttg
     4621 tctagcttca ttgaagctat caaattgttt tggcaaattt gtcatgaaaa tattcctccc
     4681 aattacttct cttcgtaatt caatttgtat cttaagcaat aaaaactaaa tttttccatt
     4741 aaaatacaaa cctctacatt agattctatc atgctttacc ttttatccat aattgttttt
     4801 atcaatgcaa aattagatac atatagatat attgtataac taatttttta tgttataatt
     4861 tctcttataa cttaagctct cgaatcctca atctataagc agacacagca gtttcacata
     4921 atttatcaat taattttcca ttcacataaa atcctacaaa tgctcctatt ccaggcatta
     4981 gttgtaaaaa cttagctaaa tctaaataat ctctatactc ttgttgaaat gttctccaat
     5041 caaaatcatt gatgtcttct ggtagactat ttacatattc atcccagtta ttcatcaatt
     5101 taaaagtttt gtttatatgc tgttgactag aaaaagccaa ctgtaatatg tttaatatat
     5161 aaatcctttc tctataatct ttaacatcat atccatatat tgaagctaca tcatataaaa
     5221 atttcacttt aatacctaaa agcaaaggaa aatcagataa acctaagagg attcctcctg
     5281 cccctgttcc tgccccttca atcattgctg tggtcttgta agttttagct ttttctaaaa
     5341 ctaatttttc tctttcttct aatgacatat ttctaagtgg tggctttgta gcatatttat
     5401 atccaaacag tacggttttt gtcattactt ttatagaaga agtaattatt tcatgatatt
     5461 tatctggcaa tactccatta aatttattct gtataccctt tgatgctttg tcaaatatag
     5521 aaggtttctt taatatattt ttcttccatt cttcaagttc ttttttgact agtttaatat
     5581 aaacaacatc ttcataatct ttcacatttt ttctcctaat acaatgtatt tttgtcaatt
     5641 taatagctta atttaaagtt taaatatttt tatctattaa attaaactat tcaaatactc
     5701 tttagtttct ttatctgcaa aactagcttc tactgcattt ctataaataa ttttatagtc
     5761 ttctaatcct agtttaaaag tatcaaatat aacatttagt tctgaactta aatttatatc
     5821 tgatacagtt ctattatcag tatttatact tacatgtaaa ttatctttat aaaaagtata
     5881 aaatggatgt acctcataag aatctactgt cttagtttgt acattactag ttggacataa
     5941 ttcaagcaat atttttttat ctttaactaa attatacgcc tttttatggt ctttaatttt
     6001 taccccatga ccaatcctat ccgcctttaa aatatttata gcatccaata cattttctcc
     6061 acttgcagct tctcctgcat gtattgtaac tttatatcca tattctcttg ctaatttaaa
     6121 aacatcttta tattccttac aaaatccctc tttctctgga ccacacaagt ctactgctac
     6181 aacaccttta tttacaaatg atttcccttc ttcaattact aataatgcct cttttgaggt
     6241 cattgttctc atacaaccta atattaaatt gccttttata tcataaatac tttcagcttc
     6301 tctaattcct tcaattatac cctcaattat atttttaaca ctcatacctt tttgggtgtg
     6361 aagtaatggt gcaaatctaa tttcaatata ttttacattt tctctacttg catcttccaa
     6421 aagttcaaaa gtaattcttt ttaaattttc cttagattgc attactttta agggtaaatc
     6481 aaatttttct aaatattcat ctagtgaagt acaattaaaa gaaacctttg ctaatttttt
     6541 tatttcatcc atattatttg aaggtaaatc tattttctct ttaatagcta tatcaagcat
     6601 ggtttctact ctaacacttc catctaagtg gcaatgtaaa tctatcttag gtaaattttc
     6661 aaacatttta tcctcctact gtacttataa ataatactat caacagattt ttaagtctta
     6721 tttctattat atcaatatac tacggttttt cattctaaaa aagtcacatc tcattaaaat
     6781 attttaattt aatttatgac tatctaatta tatatactga ttctttttaa tatacattta
     6841 ttaatataca tttgataata aaatttcaat acataagaat ttaagttata taattaaaac
     6901 tttgaattac aaaggaggac ttattttgat taccttaagt tttgatatag tgcaaacctt
     6961 gacactatct atattcttct ttttaattgg taatttattg aagaataaag ttaacttttt
     7021 aaataatttc tgtatacctg caccagtaat aggaggtcta ttattttgtt ttctaaattt
     7081 attttttaaa tattttaata tagcagatat atctataagt ggaaatttaa tgccaaattt
     7141 tataaccttc ttttttacaa ctataggact tgaaataagc ataaatctta taaagaaagg
     7201 tggtagtgta ttatttagat attggatttt gtgtggagta ttggcatttt gtcaaaatat
     7261 attagctatt acaatatcaa agattataaa acttgaacct cttttagggc ttatgtgtgg
     7321 aaatgtatct atggaaggtg gtcatggtta ttctgctgca tttggactta caatagaaaa
     7381 tctcggtata gaaggagcag ttggggttgg attatctgct gcaacaatag gacttattat
     7441 gggtggtata ttgggatgtc ctgtagctaa atttctaata aataaatata aattaaaacc
     7501 atcatcaaat ataggcttgt cgattcctag atataataga aatctaaaga gatttggaag
     7561 taaatttttt agaaataaga ataggataaa aaataataac ttctccgaaa caataacacc
     7621 atctgtattt ttggaacagg ttcttctcat atttatttgt ataaatacag gagaaattat
     7681 aagtagatgc ttctatataa cgtttaatat actattaccc tctgttgtaa cttgtatgtt
     7741 ttcagctgtt atttttagaa atttaaatga taagataaat atattagaat taaattttaa
     7801 acttatagat tttttaaaag aactttcatt gggaattttt ttaactttat cacttatgaa
     7861 tatagattta tttgaattat ctacattatt acctcctatt ctcttaatag taacttttca
     7921 agtaatcttt ataatcctat tttcaatatt tatttgcttt agagtacttg gaaaagattt
     7981 tgattctgcc atcataataa gtggtttgat tggacatgga ataggagcta ctccgaacgc
     8041 tttggcaaat atgagttctt tgacacaaaa atatggtgat tctccaaaag cctttttggt
     8101 tgtaccatta gtaagtggtt ttttgctaga tgctattagt atcccatgta tactattttt
     8161 tataaacata ctaacttagt atgtttattt tttgttatat attgacaatt tactcttttt
     8221 gttatatatt ataaatataa agtttgataa tgattttcat taggaggtgt taataatgac
     8281 tgtttacaac ttaaaacttg gacaaaaggg aattatagac aatatagctg gaaatgaaaa
     8341 acttatgaag agacttctag ccttaggact aatagatggc actgaagttg aagtgaaaaa
     8401 gatagctcct cttaatgacc ctatagtgat tagatttaga gggtttgact tagctattag
     8461 aaaatctgat gcaaaaaata taaatttaaa aaataattaa tatataataa aaagagattt
     8521 taaaatctct ttttttgttt atgtataatc ttctatttat ttttttcatt ctttatactt
     8581 taattggctg ttttactata aatattttta ttacttaatt gctttcttca ttccatccat
     8641 agcaaaaaga tatcataaaa taattttcaa aaactatttt atgatatctt tttttatttc
     8701 tatattatat ttttattatt caaatactgt ttgttcttct atatcctctg ttagtgcatt
     8761 taaagctttt aaaactatat tctttctaat ttttttactt tcctcttctc ctaacatagg
     8821 atttccaagt ggatatggta tccctactgc tggaacaatt ctattagctc ctattgtaag
     8881 tgatattggt actactgtac atatatgaac aactgctata ccagctctct caatctcttt
     8941 taccatcgtt gctccacaac gagtacaagt acctcatgta gaagttaaaa ttacagcatc
     9001 tacatcatct tctaacaatt tcttagaaaa ctcttctcca aatctcttag aattgccaac
     9061 actcgttcct gttcctgtag tagttataaa ataatctact aattctccaa ttacaccttc
     9121 tttttcaagc tctctaacta catctaatgg tacaactaaa tttggatttt caaggacata
     9181 tgctctgtca taccccccat gtatagttgt aaattcttta tttgacatag aatccatatc
     9241 tgttaaatcg taaattccat atttagtagc acttgaagat tcaattttgt ctggattatc
     9301 aattggaact atccctccag aagttactag ggcaagtttt atcttagata aatctttaac
     9361 aggtttcata ggtgtaactc tatcaaattc aggcattgga tactcagttt caaagctttc
     9421 ccctttcatc tttttaacca acatatcaat tgctctttca gaccctctaa gttcatggaa
     9481 gaaatttttt cttattcctc tcattatata tccttcctct tcaggaggtc ctatttcctc
     9541 acctctaacc aatttaagac ctagtttcgt cattattgga actacctttc taagacttgc
     9601 tgctgaattt cctgtagaca ctatatgcaa atctaattta aacatatcta cacctggatt
     9661 ttctttatac attcctgtaa ctacaggaat atttaactct tcttcaactg ccttacaaat
     9721 agtcccacaa gctactccat atctacctgc attaaatgct gggcctgcaa taaataaatc
     9781 ggggctttct tcttttatca tttttaaaag ttcttgagtt gcactctcaa tattttcacc
     9841 aaaatacgta tctccacaaa ttacagttga gattatctct gcatcatcat taaattgact
     9901 ctgaaatgct attccaggtc ctactacacc agcccttttt tctggcggaa tatctgcttt
     9961 ttcctctcca cctataccag caaagaaatt gtttatataa tgaaccactc tgattttact
    10021 catctcttaa tcctccaaat cactttatag ttattacatc aattagacca acgattacat
    10081 caacagaact ttatattcaa atacttattt aataatatct tgctgtcaaa tgattatagc
    10141 catttgctat agttgatgct attataattt gtagctctgc atccatgctt ccgtcttcat
    10201 ttaagcatcc tttataccca cctatcatca tttcaacata atctaaagtt cctataattt
    10261 tttccattgc tgggaaatga accactagat ttccttgacc acaagaaaca actgcatctg
    10321 cttccttagt agcgtctgct atagattgac tcttgccatc ttttccagga aattcatccg
    10381 ttatcaaaac tacatttgct ccaacttcac tacattttct acaattcatc attaggtcag
    10441 tatctggatt cccataacct tcttctgtca ctagaactcc atcaactcct aaaaattcta
    10501 ttaatttagc taccatatca gagcttctct ctttatcgac taagaatacg ttctcattag
    10561 tcaaaataac tcctataaag tttaaatctt ttccatgtct tttatataag tcatgtataa
    10621 ctggattatt taaatgatgg aatgttgtaa ccttatcaca tggtgctaca cagtttccag
    10681 atattatagc tccatccatt atttcagttg gatacataaa tgtaggcact atctgtttag
    10741 catccacccc atagtaataa gtgtcatgta aaagtccttg tgattgtaac atgtgaacat
    10801 atgctactgt tggcaggtct ggatattctt ttacttgttt taataatggc tttgtctcat
    10861 atacaatgac ttcatctggc tcaacttctc ttcctgcctc acctacatat gcacctattt
    10921 ttaatcctgc aattctagct gcttcttcat aatcatgagt atctatacca tcctttgctt
    10981 ttataacaat acatatatta tttgtttttg aaaaaggtgt atatttagca gtaggaccac
    11041 tcatatcaat tacgccttct tgaaatccaa ctatatttcc acaagttaca acacaagctc
    11101 ctaaaagtac atgggttctt ccacttccta cagtcttcac tttatttata attccaggaa
    11161 atattttgct ttcatctcca attttaactc taggctctat tacatcttta actggagcta
    11221 ttcttattga ttcgccaggt cttgcaagtt caacatttac acttacaatc ctttcatctt
    11281 ctaatactat cttttctatt tcttcgctac taatatatag tactccatct tttacttctg
    11341 tcttttctcc aaattcaaca ttttttataa atatattccc tagctctagc ttcatatgaa
    11401 aatccccctt aatatattta atcgcaatta aaaatattta ttacgtttat taatatataa
    11461 agcattttct atgccaatta tttaaaatat atattctatg tattaatgtc atagaaattt
    11521 tcatttttaa tttacaccag tattgaattt accaataatt attgtaaata ttaattttta
    11581 aaattataat aaaaaaagat aaacagattt tttctcttta tcttttaata cagtttgaga
    11641 aatttttttc tcacttaatg agaagttttt ttctcactta atattatact ctgatatttt
    11701 attggttaac tgtcttctac taatctccaa ttttttagct gtatttgaaa tattatttcc
    11761 acacaaagat aaaacttttt caatatactc acattcaaac tcttttctta tttctcttaa
    11821 aggtctaata cttattttat catctatgta tacattattt gatattaaat ttaaattgtc
    11881 ttttgataaa ttgccttctt cacttaaaac tacaagtcta tttattatat tttttaattc
    11941 tcttatattt cctggataat tgtaatttaa taaaaaatcc tttacttcct tatcaatcga
    12001 gtgtatcttt ttatcatgtt caatttggta cttatttaag aaaaattcta taagagtagt
    12061 taagtcttct cttctctttc ttagtggtgg tatagttatt gttattgtac ttattctata
    12121 aaaaaaatct tctcttatat ttccatttga tatttccact tttggttctt tattcatagc
    12181 acaaattaac ctaaagtcaa ctttaataga tttattactt cctatcctct ctatagattt
    12241 gtcctcaagt gtcctcaaaa gttttacctg aacatttaga ggtatatctc ctatttcatc
    12301 caaaaataga gttcctttat tcgaaagttc aaatctacct tttctactat ctactgctcc
    12361 tgtaaaagaa cctttttcat gaccaaacaa ctcagactcc aatagatttt ctgaaaaaga
    12421 acaacaattt acaggtacaa atatctcatt ctttcttgga cttatactat gtatatatct
    12481 agctaatata tcttttccta caccagattc ccccaagatt aatatattaa catctttaca
    12541 agcagctttt tttgctattt ttatgatatt attaaaatct ctatttttag attccaaagt
    12601 aaattcaaaa ttattttttt gtacagaaac agatatttta cttgccttta ccttttcaac
    12661 ttcttctaat aaattttcaa tgggattact ttttataaaa taagataaag ctcctctttt
    12721 catagcatca actgcatttt caatacttcc atatcctgta actaatataa cctctatatc
    12781 tctattaata gatttaattc tatctaatag ctgtactcca tccatatttt tcatcataac
    12841 atcagataaa accaaatcat aatttttatc ttttttaatt atatttatag cttcttcacc
    12901 acttagtgct atatctacaa gatagccctt cttttgtaga atttttttca ttaccactcc
    12961 atattctagc tcatcatcaa ctaatagtat tttcattata tagtcacctc tttatttaat
    13021 ggaattttta tatgaaaaca agttccaacc cccaatttac tttcaacact tatatctcca
    13081 ttattctttt taatctcatt ataagtaata tataaaccta aaccagttcc ctcaccaatt
    13141 ggttttgttg tatagaaggg attaaatatg tcctttaggg catcttcttt tattccttca
    13201 ccattatctt taaaatctat aaataaacaa tgattttttt cataacagta tataataatt
    13261 tttccatctt gatgtattgc atctattgaa ttagaaatta aatttataaa tacatgtttt
    13321 aacgattctc catttatata gcatactaaa ttatgttcac agtcaatttt tatttcaaca
    13381 ttctttaact gtaacatttt atattgaagt ttcactatat tttcaataaa atttcttata
    13441 tttatatgtt ctaaattatc atctgatatt cttgcgaaat ttaacaagtt ggttattata
    13501 ttacttgccc tatctacatt actttctatt gatttaacac aatcattcac ttcttccatt
    13561 gtaacgttat cttttaatag atagcaatta tttcttatta tacccaaagg atttcttatc
    13621 tcatgtgcta ccccagaagc taattgtcct atagatatca ttttgttttc tctaagtaat
    13681 ttcgcctcta ctattttttc attagttata tctttaataa gaactactat atactcagta
    13741 ttattttttt tatactctac aggaaaagtg tttatcttta aaattttatt gtatatttct
    13801 atttcactag ttttattagt attttcagag aaggtatttt taataacacc ttttatcatg
    13861 ctaatcaaat ctgctatctt ataactcatt tcacccataa agtcatatag tactttgttc
    13921 gattctacta tattgttatt ttcatctaca agcattatta tatctgtaat actattaaaa
    13981 gttgcctcta aagttttttt attttctatt acctgttctg ttttcctttt gatttctatc
    14041 tttaacaaat atgtatagct ataaaaaaca taaattgata ttaaaattat acctataaaa
    14101 agataaatac taggcacaat tcctcgctca tacaatatat catcaacttc attatacgta
    14161 gaataccatt ttttctttaa atctttatac actccatttt tctgaagttt aaaaatgcct
    14221 ttatttaaaa tactaaccag ctcttcatat tgtttaggta cagcaagaac tgctttttta
    14281 gtatatatag gattacttaa aacactgtat ttatttgaaa gtccataatt atttatatag
    14341 tatcgcaata ctggttcatc accaacaact gcgtctactt ttccagacat taaatggttg
    14401 actccagtct ttatatctgg agtcaataaa atgtttatat cttgaatttt ttgctttaaa
    14461 aaatttatag aataatcacc ttctggaatt gctactgttt tcccctttaa atccatgtaa
    14521 ctatttatat cttgggactt tttatctttc aatatatttg cactcaaagt gtatattggg
    14581 tcagtaaata taaatttatt tgccctttct tttgatggaa tcaagtcaaa aaactttata
    14641 ctatcatcta ttgaatttac gaaagattct ttccaccaat tatttggttt aaaataaaaa
    14701 tctctaccaa tttgaattga cagagaattt attaaatcaa caataattcc cttatactgt
    14761 ccatcttcct tacccttata tctcaaagga ggtgaacttt ggtcagagct gtaaatcaaa
    14821 ttcttgtgtt tttctaacca ctttttttct tcttctgtaa atggaagtga cttcttaata
    14881 tattcaaata catttaaatt atactccata tttacataca tatttgtaag tgaaaatact
    14941 actaaaaatg aataaataat ccctataata acaatctttt ttttattcac tttaaaactc
    15001 caatcttctt ttttactata ataaattcaa tatttaataa caaaatccta cttttttcat
    15061 taaatattta tatttattat aaaactaatt gaatagatgt tgaaattata gtcattacta
    15121 ttcctatcaa tgcttcatat ggaattaact taaatctttc acctatatcc atgtttatac
    15181 tacctgaact agtatgaaat agtgagccat gtggtaattg ctcgaataca ctagacccag
    15241 catttactat tgatgcacct gaaatcggtg atagcccacc atttattata gcatcatgaa
    15301 aagtagatga tgctatagta gcaccagctg ttgatgatgc tgttgctaat gacataagta
    15361 tacccgaaac tggtgccaat aaaaattgtg gcatatttaa aaattgtaaa gcacctattg
    15421 tactctgttg taattcagac atctgtataa ttcctgcgat agtccctgtt ccaagtaata
    15481 atatgcatac cccttgcatc ttagataatc caaaagctaa atattctctt gaattgttca
    15541 agtttcctgt gacaattaga gttacgattc ctcctattgg aagggctatc attgggtcta
    15601 taactatagg agatacattt cctaaaaaca atagaaatat agctataatt ggaccacata
    15661 atgatttaaa taaacttggt aaatcttctc tttgctctaa aatttcatat gattgtactt
    15721 tatttccttt atttattaat atacttgcta aaataactgt aataacaact ccaataattg
    15781 ctggaattat atttgccatc ataacactag agagattgac agaaaaatta tctgctactg
    15841 ctatagtatt tggatttggt gatattatat tacctgcttt accccctccc aacatagcta
    15901 atagtattga caatttagaa taatttaatt ttctacctat atataatcct ataggtgcta
    15961 cagttattat agatacatcc aaatttacgc ctactccagc aagtaccata gttgaaatgg
    16021 caatcgaaaa taaagccctc ttttcaccaa atatttttac aatctgttct gatattttat
    16081 ctactgctcc tgtttttatt aaacttccag ctaatacacc tgatgccaaa gctctaagaa
    16141 ttgatggtga tatattcata gctccatttg ccataaaatc tacagtttgt gttatatttg
    16201 ctcctccaac taatcctcca ataaatgctc ctaacattaa actatataca gcttgaaatt
    16261 tttttataat taaaataatt gctattaata aaccaaatat agctccaaaa gtcgttattt
    16321 gcataatgtc ccctcctatc ataaattaac tatcttttgc ttaattttat ttaaatatat
    16381 tttaagtatt ctgtatccac tttgtcaatt atattttaat ttatttaatt atattgaaca
    16441 tagttttttc aaaacctaat tctatatgtt ataataattt catactacat aatcatttta
    16501 cttgattatt ttaagttaat attttttaaa ttaaaaagag aggatattta tatgaatgat
    16561 ttaaatttag gggaaaaaat agctgaagtt cgaaaaaaac aaaacttaag tattcgagat
    16621 ttagcaaaac tagctgatgt tacaccatca ttattgagcc aaatagaacg aggtcttgct
    16681 aatccatctg taaattcatt aaaatctata gcctcttctc taaacgttcc tttatttaca
    16741 ttttttgtat ctgaagtaga taaaaaaaac ttgatagtaa gacatgataa tagaaaaaaa
    16801 gttattttac ctggaagcaa agaagttata tatgagattt taattccaga ctctagtggt
    16861 aatttagaat ttgcaattat ggatttagct ccaaatactt cttcttgtat tgataggatt
    16921 actcataatg gagatgaaat tgcatatgtt ttggagggag aagttaaact ttttatggat
    16981 gatgatgaat ttactttgtc caaaggagat agtgtaaaag ttcctttagg aacaaagcat
    17041 aaatggcaaa atgactcaaa tagtgaaagt aaagtaattt ttgcagtcat cttgtaaagg
    17101 agtaaaaacc atgaagacat ataaatttat attttttgat gcagatgata ctttatttga
    17161 ttttaagaaa agtgaatcac atgcttttaa aaaacttcta tctgagtttg atttagaatt
    17221 taattttgag aattatatag agtcatatag aaatataagt gataagcttt ggctcgacct
    17281 tgaaaaaaat attataactt tgaatgaact taaattactt agatttgaac tttttgcaaa
    17341 taaaatatct ttggatgttg attctgaaac tcttagcaaa atgtacttaa attttttagg
    17401 tgaatgtaca tttttaattc caggtgcaat cgatatatta caatatctta aaaaaaagta
    17461 tactattgtt ataataacaa atggaatttc tgcagttcaa aagaaaagac ttgaaaattc
    17521 aaaaataaaa ggatatattg atggaatggt tgtatctgaa gagttgaaaa tatcaaaacc
    17581 aaatcctgaa atatttaaat acgctctcaa aaagtttaat tgtcatgata aatcttctgc
    17641 cttaatgatt gggaatagct taacctctga tgtcttaggt ggaataaatt caggaataga
    17701 tacttgttgg ttaaattcca ataacagtat aaattatact aatcatatac caacttatga
    17761 aataaataca cttattgaat taaagaaact actatagttt gtaagtctgt tcaaattaaa
    17821 tagaatatta ctttgcaata atttttttag tttatacata aaatgaccaa aaaaattatt
    17881 gcaattcata cacaaaacaa atcttagtct tgaaaactag ttaaaagcat tttctaggac
    17941 atttgctaca tcttgattct aaattttctt catctcccat taaaataagc attgcaacac
    18001 tttttatggg ggatagttga tgataatcat tgatttctat ttctatacta ttatatttat
    18061 tttttacata gttataaata tccttttgtc tctctacttc aaaatcttta tctcctggat
    18121 atatttcatg cgatatttta agactatcat actttccttt taaatacatt tttattttct
    18181 cattaatata atctaaacat actataccta ttttatccaa tgctatccct gccatcatat
    18241 tgcaattttc catatagtca ttcatcttaa tatcaatttt atcccctatt gtatatagta
    18301 cagcaaatgc ttttttacat tctttaggtg ttttattaat atcaacttca cttatatata
    18361 tcttgatatc taaaatatca tttatatttc ttatttctct ttcaacgatt tcactaattg
    18421 tatctggagc ttttctattt ccatacccca aaaactttag cacaatattc ttatctataa
    18481 aaatatcctc taaatttata tcctttaaaa gatatttatt tttattacta ttataattca
    18541 tcaatatcaa tcctatctaa ttctttataa ttaattcttc ctgcttttat agctgcttgt
    18601 aaattttcta ctggagtggt atttaagcag ttacagccaa aatcacataa aaagttatca
    18661 tatcctctca tcttctttaa caattttagt gtctctcttc taatcttatc tggcttagaa
    18721 cgtccaagaa gttttattgg gtctaaatta ccaatcaaca ctatatctct aggaatcatt
    18781 ggagcaattt cttcatagtc taaaatttgg tctaaactta ctgcatctat attacaactt
    18841 agcatattat ctaataattc tctggtgtct ccacatatat gcataccttt ccaacaatca
    18901 agttcattgt agattttatt taaatttttt actatatatt catcaaatct atctttactt
    18961 agggttactg acgttggctc tgctattgaa atatattttg ctcctgcttt atttgcagct
    19021 acagcatatc ttcttaccaa ttcagttgta aattccaaca attcttctac aaatttttta
    19081 tcagttatta tacatcttaa aagttgagta gctccagcta attgacctgc aagtgtaaat
    19141 ggaccttgta tcgattcata tagtggtttt ttaatacttt ttgatataac tgataagctc
    19201 tctatattga gtggcatacg tccactcgta taagggtctg gaacatctaa ttttcttaat
    19261 ttatctctgt cagttatagt atgtgtcaat acacttggaa aatcataatc tggtcttaat
    19321 gtctctaaac ccagagtttc acaaaatgta atcccatcac agaatgagta tataaaatct
    19381 gattcaaaat acttgtccat agtctttgct aattctaatt gcaaatctac actatcatat
    19441 acttcatatg cctttttatt tattaagaac aatccatttg tacccatatc aggcagaaaa
    19501 aaacctctat cttcactatt tatatactga agtaaagtca tatctgaaaa catttaaact
    19561 tcacctccta taagtttgtt tattaattta actgcttttg gagcatttga actgtatcca
    19621 tctgctccta tttcttctgc aaactccata cttacaggtc ctccaccaat tattactttt
    19681 ggtcttttat ctatctcaat catattaagc tcgtctataa ccgatttcat attttccatt
    19741 gtagttgtca tcattgaaga taagcctatt atatctacat tgttttttat agcttcttct
    19801 attatatctt cactttttct atttacacct aagtcaataa ccttatatcc agtggcttct
    19861 accattactt ttacaatatt ttttcctatc tcatgagtat ctccttctac tacagacatc
    19921 agaattactc cctttgattt tttctttact tttccagttc ccttcaaaag attaatacct
    19981 ttatttaatg catctgtgca tactataact tctgaaagat agtattcctt attctcaaac
    20041 ttatctaaag ctcctatcat acctttgttt aaaccatatt cataaatatc ttccacatca
    20101 atatttttat ctatagcaat ttttactaaa ttctctatat tatctacacc catatttaat
    20161 atacattctg ctatatcatc aagtatgtcc atataatact catcctttct aattacttac
    20221 aaaattgctt tttcaatatc caataatttc tcttatttaa caaggactaa ctctaggtat
    20281 caatcaattt tatttaatat catctaaaac tattgataat ttaaatttgc agtccatgga
    20341 aaatctacag gaaatgcctt tgtagcaaat tcatatatat tttttagatg tttaaattca
    20401 ttatactgtt cctcattttt ttcaaaaaac agtttattaa gcttatccaa attttctatt
    20461 acaagttctt cttctttgta tttaataata tatttatttt caacttgttt aaattccatg
    20521 tattttagta attcaaaatc tacatattga ctaaaataac ttcttaaact atcacacaac
    20581 ttagtaaaat ttattatttt catagttcct tgttgatagt ccaaagattc tgtcccatta
    20641 catttcaact ggtctttaag atttttcaca tgtactttat aatttaaata ttctaatcca
    20701 aatttatttg caacatattt caatatattt tcaacattta cagagtttaa ccctatttca
    20761 gcaacttctc ctacagtaga atcttctttt tttattgttc ttattatcaa atatcctatt
    20821 atattattat tttcctttat aaataccttc ttgtaaccaa ttggtcccca ggcaatagtt
    20881 gctgcatgca atagtttttg aaactcatct ctcgttctta taaatctagt actattttga
    20941 ttatatagtt caagcatttt atctaaatca tcatcttttt caaaattagt ttcatcaaat
    21001 tcaactattt catatgctat ttttacatct tcaggtttta ttgtatattt ataaaaactc
    21061 tttaccaaac tacaatttct tctactgtat agacttcttg ttcctgatat aattacaata
    21121 tctacaccat cataaaacat tttttcttct actttatcca atatcttaga tgaatagcct
    21181 ctcttttcat aatctggatg agtacatact ccacctatag ctgcaacctt caatctattt
    21241 ccttgaatag atacatcttg aattagatag tttacatcag aaaccacttt atcatcttct
    21301 gatattataa tcatattttc tatattattt ttacttagta aaagaggaaa ttcttccatc
    21361 atagttggct tatgatttct tgatattcta aatacatagt ttattaattc tattacttta
    21421 tcaaattctt ctaattttgc acttcttggt cttaattcca ttcatattcc tccaaatatt
    21481 atttaag
//
LOCUS       DAMKPG010000069        20894 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMKPG010000069 DAMKPG010000000
VERSION     DAMKPG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.072"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            81..902
                     /locus_tag="PC621_002572"
     CDS             81..902
                     /locus_tag="PC621_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF4109719.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            923..2119
                     /locus_tag="PC621_002573"
     CDS             923..2119
                     /locus_tag="PC621_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF4109720.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            2285..5683
                     /locus_tag="PC621_002574"
     CDS             2285..5683
                     /locus_tag="PC621_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF4109721.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5693..7366
                     /locus_tag="PC621_002575"
     CDS             5693..7366
                     /locus_tag="PC621_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF4109722.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7640..7834
                     /locus_tag="PC621_002576"
     CDS             7640..7834
                     /locus_tag="PC621_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF4109723.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    8003..8236
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8313..9710
                     /gene="asnS"
                     /locus_tag="PC621_002577"
     CDS             8313..9710
                     /gene="asnS"
                     /locus_tag="PC621_002577"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF4109724.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9883..11058)
                     /locus_tag="PC621_002578"
     CDS             complement(9883..11058)
                     /locus_tag="PC621_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4109725.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11477..12841
                     /locus_tag="PC621_002579"
     CDS             11477..12841
                     /locus_tag="PC621_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF4109726.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12863..13354
                     /locus_tag="PC621_002580"
     CDS             12863..13354
                     /locus_tag="PC621_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF4109727.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13627..14298
                     /locus_tag="PC621_002581"
     CDS             13627..14298
                     /locus_tag="PC621_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF4109728.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14383..15249
                     /locus_tag="PC621_002582"
     CDS             14383..15249
                     /locus_tag="PC621_002582"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF4109729.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15282..16784
                     /locus_tag="PC621_002583"
     CDS             15282..16784
                     /locus_tag="PC621_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF4109730.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16914..17366
                     /locus_tag="PC621_002584"
     CDS             16914..17366
                     /locus_tag="PC621_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF4109731.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17526..18329
                     /locus_tag="PC621_002585"
     CDS             17526..18329
                     /locus_tag="PC621_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109732.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18387..19274
                     /locus_tag="PC621_002586"
     CDS             18387..19274
                     /locus_tag="PC621_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF4109733.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19293..20141
                     /locus_tag="PC621_002587"
     CDS             19293..20141
                     /locus_tag="PC621_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4109734.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20204..20893
                     /locus_tag="PC621_002588"
     CDS             20204..20893
                     /locus_tag="PC621_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF4109735.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         8190 a         2223 c         3745 g         6736 t
ORIGIN      
        1 ttgtattatt atttttatat caaaaatagt aataaagtct atatacattt agaaaatact
       61 gtttaaaagg agtaggattt atgataaaaa aagagatatc taactttaga ggaagtagtg
      121 attatgtagt atcaccagaa ttgatggcat cagttaatgt tgctattgct cttgaaaagc
      181 cacttttgat taagggcgaa cctggaacag gtaaaactat gctagcacaa gcaatttcaa
      241 atgaattaaa aaaagattta gtaatttgga atataaaatc aactacaaaa gcacaagaag
      301 ggctttatgt atatgatact gttcaaagac tttatgatag ccaatttgga ggagaaggtg
      361 tagatgatat tagcaagtac ataaaatatg gtaaactagg agaggccttt agctcaaatc
      421 aacaagtaat tcttttaata gatgaaatag ataaggcgga tttagaattt ccaaatgact
      481 tgctttggga attggataaa atggagtttt acataaatga gacaaaagaa actgtaagag
      541 ctaaacaaag accaatagtt attataactt caaatgcgga aaaagaactt ccagatgctt
      601 ttttaagaag atgtatattt cattatatag aatttcctga tagagacatg atggaagaaa
      661 tagttaaagt acattttgat aaagtagaag aacacctatt ggaacaagta atgactactt
      721 tttattggat tagaagttta aaggacatac aaaaaaagcc tagtacatca gagttaatag
      781 actggataca agcattaact ttgagtggaa tgcctattga aaaaatagaa aaagaagttc
      841 cttttgcagg aatactactt aagaataatg aggatattga atctatgcaa agacacttat
      901 agttaatagg agctggataa ttatgtttat tgaatttttt tatttattga ggtcaagagg
      961 attaaatata tctttaaatg aatggatgac tttaattgaa gcattggata aagggctgtg
     1021 ctattcaaac ttttcaaatt tttactatct ttgcaggatg attttaataa aaagtgaaag
     1081 tgattttgat aagtttgatg cagtgttttt ggagtatttt aaaggtatag aacaccaaga
     1141 agaaattcca gaagagatta tgaattggct taaaaaacca gatatagatt tagaagagtt
     1201 tgaaagattg gaacaaaatc caaatataaa tcttgataaa ttaagagcta aattggagga
     1261 acgaataaaa gaacaagact ctcctcataa tggtggtaat tattggatag gcacaggagg
     1321 cacttccgag ttaggacatt caggaaaagg gcaaactggt atacgtatag gtggaaattc
     1381 tacttatggt agagctgttc ttgaagttgc aggagaaaga aaatacagag attttagaga
     1441 tgatgaagtt ttaaatatgc gtcaatttca agttgcacta aagagacttc gtcaattttc
     1501 tactagaata gatgctccaa agacagagct tgatttggat aagactattg aagagacttg
     1561 taataatgca ggatatttaa agttattttt tgagaagcca agaaaaaata cagtgaagct
     1621 tttattgctt atggattcag gtggttcaat gagggggtat agcactttat gtaatacatt
     1681 atttcagtct gtaagtaaat caaatcattt taaagatgta aagatatatt atttccataa
     1741 ctgtatttat gatagattat ttacaacacc tgaatgttgg ttaagtaaat ctattaatac
     1801 tgaatggata ttaaaaaata tagataaaaa ctataaagtt ataattgttg gagatgcttc
     1861 tatgtcgcct tctgagttac ttcatattgg tggtaactat cgtggtccat ataattacac
     1921 acctggtata gaatggctta aaaggtttaa gagaaagtac agtaaaattg tatggatgaa
     1981 tccagaactt agagatggat gggattcaat taattattgg taccaaactc aaagaatgat
     2041 acaaagtgaa tttaatatgt ttccactgac tgtaaaaggt cttgaaaaag cattgaaaaa
     2101 tttaatgctt tcaagataaa gtaaatataa atgatggtat gatttattca aaattgtacc
     2161 atcattattt attttttaat atatctatta taaattgtat aactgaaact taaaaaatta
     2221 tatagtctaa tacattttag acttacacaa aacatataca ttaataataa ttttagaagt
     2281 cgaggtgatt ataataaatt atgaattaat tgtaaaatac aatggagata tattgagatt
     2341 agaagaagaa ttaggtgtat cagtagagat acttaattca tcatatgcaa taataacatc
     2401 aagtaatgaa gaagatgtaa atacactttt aacttatcca gaaattgaat ttatagaaaa
     2461 gccttttata ttacagactc aggatgtgca aagtttttca agtacaggaa taacggggtt
     2521 taaaaataga actggtttaa ctggaaaggg aactataatt ggtataattg attcgggaat
     2581 agattatact ctacctgtgt ttagagatag tgatgggagg tctaagatat tatattattg
     2641 ggaccaatct attcaaggaa atccaccaga aggttttaga gaaggaacat tatatactaa
     2701 tgaagatata aataatgcaa tagatggaag tatgtatata cctatttcaa caactagttt
     2761 acatgggaca catgttgcag gtatttgtgc aactattgca agtgatgcca gaataatagt
     2821 ggttagagtt ggaaatatac agaccgatat tttttcgaga agtacagagt ttatgagggc
     2881 aattaaattt attttagata gggcattaga attaagaatg cctgttacat taaatataag
     2941 ttatggaagt aatgaaggtt cacatagagg aacatcttta tttgaacaat atatagatga
     3001 tatgtgttta ttttggaaaa acaatatagt tgtagcagct ggaaataatg cagataaagg
     3061 aggtcataaa aggattaggc ttcaaaataa tattacagaa gaagttgagt ttatagttgg
     3121 ggaaggagag cgtatattaa acataaatat atggccagat tttgttgatg attttagtgt
     3181 acatttggta aacccatcaa acaatcaaac acaagcaatc tctctaactt caggtgagat
     3241 aagaaatact ttgggtgaaa ctagaataac tggttatttt taccctatag caccgtactc
     3301 tcttacaaga agagttactc tacaattaag ttcaaataca cagataactc ctgggttatg
     3361 gaaaattgta tttgaaccaa tagatatagt aactggtaat gtaaatatat atttgccaac
     3421 ttctgaagga ctaaatagaa atactagatt tctgattcct actcaagagc ttactgtaac
     3481 agttcctgga acagcaagta gagttgtaac agtaggtagt tttaattcaa gaacagacat
     3541 agtatctata ttttctggtg aaggagatac acaattaggt gtttttaaac ctgatttatt
     3601 agctccaggt gaagatataa tatctttttt accaggaggt acaagtggag cattaactgg
     3661 aactagcatg gctacacctc atgttacagg ggtttgttca ttgtttatgg aatggggaat
     3721 tgtaaatgga aatgatttat ttttgtattc acagaaatta agggcattac ttttaaaggg
     3781 agcaaggaga ttaagtaatc aatcttatcc aaataattca tcaggatttg gttttttaaa
     3841 tttatcagat atagatttgt acactctatc tagtataaat caagatttag aaacggagga
     3901 tattggatat agaagtatta ataagtcatt taaagatgag gaaaataggt ataagtttat
     3961 agatggttat aatatacaaa tacataatga cttagagaat gaaatttata taagtaaaaa
     4021 tgcaagtaga caatcaggaa tactatcagg aatagatgta gtccatactc ccgaatttga
     4081 agaagaacta gctgggttag gtatgagtca aaattttttt aagataagtg attcattagg
     4141 tgtactatct ataaataata cagactatag tagtatacaa cgagttttgc aacttccttc
     4201 aatcataaga actgtatcga ctacaaagat gacattactt ggagaaatta atcgaggtac
     4261 ttttggaggt gtagttgcaa cagaagaaat gggcgtaaat ttctttaaaa ataatcctaa
     4321 tattaatata acaggaagag ggactctaat atcaattgca gatacaggaa tagactatct
     4381 acatccagat tttatttatc cagatggtac ttcaaaaata gtatatttat gggaccaaac
     4441 aaaagaagga actccacctg atggttttta tattgggaca gaatatacac gagaagatat
     4501 aaatcgagct atcgcagaaa atgaccctag tctatctcag gatgaagttg gtcaaggaac
     4561 tatgttgagt ggtatatgct cagggcttgg aaatgtaaat agtgaatatg caggtatagc
     4621 agaagattct gagctaataa taataaaact cggtaagata gatggttttt ataatagtgc
     4681 aatgttgttt gctgcatctc agtatgcata taaaaaagct tttgaactta gaagaccact
     4741 agttataaat atgtctttag gtaccagtag cttggcaggt ttaaccaata ggagcaatag
     4801 tgaaaaagca ttctttacta gagggttatg tataacagca ggagcaggaa atgagggcaa
     4861 tacacagaca catacatctg gaataatacc acatgttgga ggttctgttg aagttgaatt
     4921 agagttaaat gaagacgagg aagaattatc attagaactt tggttaaata gaccagataa
     4981 agctgatgta ataatagtat ctcccactgg tgaagaaagt aaaagtgttg gaatttcaaa
     5041 ttataataaa gtaacaggtt tatttgattt agaggggact gaatattcta taacgtatat
     5101 atacccaaca actttttctg ggcagcaatt tactaatgtt actttaaaga atgcaaaaag
     5161 aggtgtatgg aagattcgat tagtaggggt ttatattata acaggaagat ataacttata
     5221 ccttccaaat agagaacttt taaaaagtgg aactagattt agagaggtag acccttttta
     5281 cactataaat tatcctgcta ttcaagatga tttaataact gtaggagcct acaatactat
     5341 taatggtagt ttatggcaaa gttcatcaag aggaccaact attgaagaca ggttaaaacc
     5401 agatatagta gcaccaggtg tcaatataat agctgcttat cctgggaaca cgtatgcaac
     5461 tataactggt acagcagcag caagtgcaca tgcagctggt gctgctgcaa tgtatttcca
     5521 atatactttt gttgatggta gatatccaaa ccaagcatat gtgcaaaaaa taaaaacttt
     5581 tatgcaagct ggtgctagaa aagattcaaa cacagtatat ccaaatacaa attctggata
     5641 tgggcttttg gatgttagag gaatgtttga tgttttaaga taggagggat ttatggaaaa
     5701 atcttattgt ataatttatc aaggtgatat agaatctgcc ctacaagaaa atggaataaa
     5761 taggtatatg gttttaaata gtcagcttgc agtaatatat gtacctgtag attttgatga
     5821 aactatttta aataatataa tacaagttgc ttggtgggaa gaatctgaac caatgagcag
     5881 tctaattgaa ataactaata atgtaaataa tggagaaacc attacaacag cagctgaaac
     5941 tgattatatt tatgaaaatc catataatga tataacagga agaggaattt tattagcagt
     6001 tatagattct ggtatagatt atttgcatcc tgattttata aatgatgatg gaactagtaa
     6061 agttctatat ttatgggacc aagaagcaaa tacaaatcca cctccagaag gttttatttt
     6121 tggaagtgaa tttacaagaa gtcagttaaa tatagccata aatagaaatg atggaagttt
     6181 gagtcaagat aatataggta ctggaacttt agtttctggt atattagctg gaaatggaag
     6241 gataaattcg cagtatagag gtattactac agaatcagac ttaatagtag ttaaattaaa
     6301 atcatataca gatacttatt atgctggaag gataaattat agtgtttcag attttttagc
     6361 agctataacg tatgttacta atatagcaag aacagaaaat aaaccactaa taataaattt
     6421 gactattgga gttaagtcaa gtgctgtagc aactacttca atattagata cttttaatat
     6481 attgagtagt gctggtgtgg tagtagtgag tggtgcagga aatcaaggga atacagatat
     6541 tcattattct ggcagattta gcagtgtggg agaagttcaa gatgtaataa tacaagatgg
     6601 agatgactat gctcttgata taacactaaa tacgaatggt ccagataaag tgggagcaca
     6661 gataatatct ccttcaggtg aagttagtca tgatataagg tattctccag acttttatat
     6721 atatagagga aaatttaact tagaaaatac tacttatgca atgagattta tttatccata
     6781 tattacctct ggaaaagaaa atttggagat aagattaaga gatataaaac ctggagtatg
     6841 gattttaaga ttgacttcag aacttataat aagtggagaa tatgacatat acttaccaaa
     6901 taaaaatttg atagctccag acacaaggtt tttagaccct gattcagtag ctactataac
     6961 tatgtatgct gctagtgatg atgtaattac agttggtaca tttaataata agactgatag
     7021 tatgtggatt ggttcttcga aaggaccaat tagaggaaga ggaattaagc cagatatagt
     7081 agcttcaggt gtagatataa tatccacata taaaaatgga acatataata caggaacagg
     7141 aacaggagtg agcagttcta tagtcacagg tgttttagcc ttattgatgg aatacttaga
     7201 aaaacaggat aatgtaccga gattatcatt gtttacacaa gttttaaaaa catatttaat
     7261 attgggtgct acaaaacttg agatatatac atatccaaat gtatcgcagg gatatgggat
     7321 tttaaattta aaaaatacga ttcaacagat agcaaatact ctatagataa gaacctatgt
     7381 aaaattataa ttgattagta atttaattgt atcaaatact ttagatagag tagaaaaatg
     7441 gcataaaaaa taaatatgaa aattaaaaaa acagttttat attttgaata cacaaagtaa
     7501 atattttgta tataaatagg tataaattaa gtattgaaaa gatatgatgt taatttaaaa
     7561 atcattataa tttagactta tgaattttat ttgtaaaatt gctaaaacta ttacaaaata
     7621 aataatagag gtgatagttt tggaaagtaa aaaggcaaga gcattaaaag gtaaagataa
     7681 taaaagactt aaaagttcac ttttagttgg aaatgagtcg acagcagcat gggctgatgt
     7741 tgaaaaatta aaacctcata gtaaagtttc aataccttca attaagggtg tcgaagaagc
     7801 aaaagactgg gttgacaatg gaagtaaatt ataaaatttt ggaaatttaa gtaataaaga
     7861 agtaatgaag ataagtattt attatcatta tgcaaggtag aattattata ttttaaataa
     7921 caatcagtat caattatata aatttattga ctaaaaattt ataaattggt ataattttgt
     7981 taattagtag aaataataaa agataaatac tgtgaagaga agagtagata taaaatgtag
     8041 ttctagcgag ttgaggatgg tgaaagctca atacgaggtt tatattgaag aacatcttgg
     8101 agtttctaat cgaaagctta tttagtaagt agacttagac ggaaggcacc gttataggct
     8161 ttaagcattg cttaactaag aggtcattat gacaactagg gtggtaccgc gagagtaata
     8221 gtctttcgtc cctttatata gggatgaagg gcttttttta ttgaaattat atttaaaaga
     8281 aattaataac aattatacta ggaggattta aaatgcaaaa ggaatttata acagtaaaag
     8341 aattgtacag agaaaaagaa cagtacatag ggaagactgt aaaagtagct ggatggataa
     8401 gaacatctag agtatctaaa aattttggat ttatagagct aaatgatgga agcttcttta
     8461 aaaatatgca gatagtattt gatgagaaag tagaaaattt taaagaggta ggaaaattag
     8521 caataagctc atctatactt gtagagggag agttagtatc tacagaaggg gctaaacaac
     8581 cagtcgaaat acatgcaaaa agcatagttg tagaagggga atcagatagt tcgtatccac
     8641 tacaaaagaa gagacatacg cttgaatatt taagaacgat agcacattta agaccaagaa
     8701 gtaatacttt ttcagctgta tttagagtaa gatcaatagc tgcctatgca atacacaaat
     8761 tcttccaaga aagaaacttt gtatatgcac attctccaat cataacgggt agtgactgtg
     8821 aaggtgcagg agaaatgttt agaataacaa ctatggattt aaatgatatt ccaaaaactg
     8881 aagatggtaa aatagactat actcaagatt tctttggaaa agaggctaat ttaacagtta
     8941 gtggtcaact aaatgccgag ataatggcac tttcttttag aaacgtttat acttttggac
     9001 ctacatttag agctgaaaac tcttatacag gaagacatgc ttcagaattt tggatgatag
     9061 agccagaaat agtttttgca gaccttgaag ataacatgga acttgcagaa gatatgataa
     9121 aatatgttat aagctatgta ttagaaaatg ctcctgaaga aatggaattt tttaatagtt
     9181 ttatagataa aggattgtta gaaagattaa ataagatagt aaactctgag tttattagaa
     9241 taacgtatac taaagcagtg gaattattaa ttgaatctgg tcatgaattt gagtatcctg
     9301 ttgagtgggg atgtgacctt caaactgagc atgaaagata tataacggag caaatatata
     9361 attgtccagt gtttgttaca gattatccaa aagatataaa agctttctat atgagaatga
     9421 atgaagacgg taaaactgtt agagcaatgg atttattagt tcctggagtt ggagaaatcg
     9481 taggaggttc tcaaagagaa gaaagagaag atgtattgtt agatagaatt aatgaaatgg
     9541 gattaagaga agaagattat tggtggtatt tagaacttag aaagtttggt actgctactc
     9601 actctggatt tggtcttgga tttgaaagaa taattatgta tatgacaggt atgtctaaca
     9661 taagagacgt tatacctttc ccaagaactc ctaaaaatgc tgaattttaa taattatata
     9721 taataaaact tatataactt taagtataaa cactaaaatg acttgcatat ttatctattt
     9781 tagagatatg caagttattt ttattgtact ataaatatat aatttgatta cttttatttt
     9841 gcgatataat acttataggt ttatataata gtatgtaact ttttaataaa atgcaacaca
     9901 attacgtccc tgttcttttg cagtatatag agccttatca caattactta taaactcatt
     9961 taatgttatt gccttttcaa ttttctttgt ataaactcca atactaacag ttacacattt
    10021 taaaggaaca tcatcacgct caaaattgca ggactctata atttttcgta aacattctga
    10081 tttaagttta gcatctgtct catcacagtt aaaaatacat ataaaaaatt cctcaccacc
    10141 atatcgtata gcatgttcat tttcagaact tgtaaaatct aaaattactt tggcaatatt
    10201 ttgtagacaa tagtctccct tttgatgtcc ataattatca ttaaattgtt tgaaataatc
    10261 tatatctatc atcattccag ttataggttc ttgatttgca tggctgatta attttttttg
    10321 tagaatatct tcaaggtatc tacggttgtt cattttagta aggtcgtctg tcataacttt
    10381 gtcgttaagt aaattattta tatgagatat ttcttcatat tgttgactaa ttaaaagttt
    10441 atcatagtaa gtaaatacac gatttctata tagcattgta gaaacaaaaa tggcaaggga
    10501 tgttataaaa aatgtgttga caatattact aaactcattg cctggtatag tttgaatccc
    10561 aggtaagaat aaattaagaa acaaaaagca tcctccaaaa actattactg attctccaaa
    10621 ttttaatact gagaaagctg cagctgctaa cataatataa ctaaatacac ttaggtcatt
    10681 tccacctaac tggtcaagta gagtaattac acaaccccat aggcagaaaa aagtcacata
    10741 aattatccat aaatttatat aaaacgtaca acaatccttt tttttattcc aaagataaga
    10801 aaatacaaac ataaagacag aagatacaca aaatagtgtt acatacatat aaaaatacca
    10861 ttggcgtctt ggtgtggcaa aagggcctcc ttctctagct gagattgata tcatcataat
    10921 tatttgagat attactatta ttatgcaaat gattttaccc gtatgataaa gataattgca
    10981 gagttctttt tcaacatttt ctttgtgttt tttgtctaga ggaaatccaa aaaatttttt
    11041 tattttttct aagttcatat aagcctccat taaaaataaa gcataaaaat aagtattcct
    11101 ttctatcatt agatatttgg aatacttcgt ttatgattta attatatcaa aatatattgt
    11161 gtgtaacaat ctaaaattgt gatagataaa atttattagc taatgttgag taactttatt
    11221 aattaatact ttactataat taagatttac agatagtttg ctgctgaaaa taaagaatag
    11281 aataaattta aattaagcat aataaaatat aaataataaa gttcgcatta acaaactatt
    11341 taaatatcaa gacctgttaa aaaacaacag caataaacaa ataaaataga taaatatatc
    11401 caattaaggt ataattaagt tttatatgat atagttatat ttgataattt acaatagtaa
    11461 aacttggagg gaaaaaatgg gtgaaaagaa aataaataat atgaattatt cacataataa
    11521 ttattccaaa aagacaggtg aaccaactag aaaaataaaa aatagccata tcactcttaa
    11581 aagtaaagaa gaaaaaattt gttctagttg caataaagaa aataatacta aggataatta
    11641 ctgtaaattt tgtggaaatg aactttatga aatagcatca ttaagaccac ttgaaacaaa
    11701 actagacttg aaatctaaaa taaaagagct ttcatatcat gcaaataaaa gaggtgtatt
    11761 tttaacaaca tttacaacca tatttatatt gtttattatt gcattgatat ttaaagctat
    11821 tataactatt caatttaatg atattagtta tttaataaat ccagcacata taatcctagc
    11881 tctcaacttg ggtcaaatta gtgtatctat gtctaccatg atgggttctg ggtttataaa
    11941 tgccaatata ggtttgttga tattacttat aatccctatt ttggtattat taatatcaaa
    12001 cctaattttt atgagaaaaa gatgtagaga ttcaaaaact gtattagcaa attctctagg
    12061 agttggaatt ttttatggat tagttttagc tattttatct atttttacta atgtaaaaac
    12121 tagttcacat agtatgttag aatatggata tgcattagaa tatagttatg aattttttag
    12181 tgttctttta aatggatttg tattagggtt tatatgtaca tacataacta cttataaaaa
    12241 aagttatgaa aaggaaaata tgtatttatc tcttttttca aattctataa gaacattcat
    12301 attaggttat gttttagtat tagtaatact attggtacta actatatctg atagtagtta
    12361 cttaaatgaa ctagatatga gtagttattc aaatggatta aatttattta ctatactccc
    12421 acaaatagct agttatatgt gggcttttgc aaatggaatt tcagtaacca taataaactc
    12481 aacagtatca atgtttactt taagttcttc atctttattt ggagatacta aattaatgtt
    12541 ttatgcaatg ggagcattgt ctatgttaat attattatta aatggataca agctaagatt
    12601 taagtataat acagatagta taagacctat aatagtattt agtatatatt atgcattttt
    12661 gatgggaata ttggctttat ttagtacatt tatactagat agtaatatta atttctttaa
    12721 tacaactaat tatggaacaa ctttaataat gcaatttaaa gtcttacaag ctattgtaat
    12781 ttcatttgta tatagttttg tgatatcttt aataggatat aagctaaatt cagctgatta
    12841 aatatgattg gagggaattt ctgtggaagg aaatactaat aataaagtag atgataaatt
    12901 ttctaaggtt aagaaaaagt ttggtagtgg aaaatttaaa gttgaattat ataaagaaaa
    12961 atcaaaatta aatagagaga ttgatgatat tcaagaaaag aaagcaaaga tattcttgga
    13021 aatgggaata ctgacatatc aaaaaattag agaagattct attaatgata atagttttga
    13081 taaattctgt gatgagttac tagaacttga taagttaatt tatgaaaaaa atatggaaat
    13141 taatgaaatg gaaatggcag aaagtaatgt aacttgtgag tgtggttatg ttggaaatgt
    13201 aaatgataaa tattgtgctg aatgtggtaa aaaatttgaa ttggaaaatg aatgtgatga
    13261 ttttataatt tgtggttatt gtgaaagtga gatagataca gaggctgaat tttgtccatg
    13321 ctgtggaaga aaaattataa tggatttaga ataatattgg gaaacctatg tatagtatac
    13381 atgggttttt ttattgaaat tatttaaatg gacttataac agtagttata taaaatgaaa
    13441 ataaacacca aaaaaatgaa ataaaatttc ttttaaatgt aaaaaacaag ctaatatata
    13501 aaataaatca gtaaaaatat agatgcgaga aaaaaatatg aaaaaatatt gcaaagaaaa
    13561 agttttcatg ttattattaa tacataaatc aagaaaataa atttcagaaa cgaggtaatt
    13621 taaaaaatgc tagataaggt aaagggaaga ttaatagtat catgccaagc tttagaaaat
    13681 gaacctcttc atagtccttt tataatgggg agaatggcta aggctgctat ggaaggtgga
    13741 gctgttggaa ttagagctca aggagttgaa gatattattg aaataaaaaa agttactgga
    13801 cttcctgtta taggtattat aaagagaaac tatgaagatt cagatatata tataacgcca
    13861 actaaaaaag aagttgatga acttttaaca acagggtgtg aaatgatagc attagatgct
    13921 accaatagag ttagaccaaa taatgaagac ttaaaagaac ttataaagta tataaaagaa
    13981 aacggtgttc tagtaatggc agacatttca aattatgatg aggctatcaa agcacaagag
    14041 tacggtgtag attgcgtttc aactacacta tctggatata ctccatatac taaaacatta
    14101 gaaggtccag attttgtatt aatggaaaga ttagttaaag atttagaaat acctgtaata
    14161 gctgaaggaa aggtaaatac tcctcaagat ttaaaaaagg tctttgagtt gggtgttcat
    14221 tcatcagtag taggttcagc aataacaaga ccacaattaa taacagaaaa atttgttaaa
    14281 gcaattgaaa ttaattaaaa attaatttat aaattaaaaa attattatat ttaactatac
    14341 ttgaaaatta aaattggagg taaagatatg agaacaacag atatgaaggg aatttattca
    14401 gcattattag tttcatttga taaggaaggg aatataaatg aaaaagggtt aagacaaata
    14461 attagacaca atatagatgt ttgtaaagta gatggtcttt atgtaggagg aagtacaggg
    14521 gaaaacttca tgctttcaac tgatgaaaag aaaagaatat ttgaaatagc taaagatgaa
    14581 gtgaaagaag aaataaaatt aatagctcaa gttggttcag ttaatttaaa agaagctgta
    14641 gaacttgcta aatttacaac tgatttagga tatgatgcaa taagtgctgt tacacctttt
    14701 tactataaat ttgattttga agaaataaaa cactattata atacaataat aaattctgta
    14761 gataatagat taataatata ctctatacca tttttaactg gtgttgatat gagtttagac
    14821 cagtttggag aattatttga aaatgaaaaa ataataggtg ttaagtttac agcagcagat
    14881 ttctacttat tagagagaat gagaaaaaca ttcccaaata aattaatatt tgcaggattt
    14941 gatgaaatga tgttaccagc tacagtttta ggtgtagatg gggcaattgg ttctacattc
    15001 aatgtaaacg gtgtcagagc tagacaaata ttcgaattaa ctaagaatga aaaaatatca
    15061 gaagcacttg aagtacaaca tgtgactaat gatttaataa cagatatatt aggaaatggc
    15121 ttatatcaaa caattaaatt actacttgaa gaacaaggtg ttgaggctgg atactgtaga
    15181 caacctatga aagaagcaac tgatgaaatg aaatcaagag caaaagaaat atacagaaag
    15241 tatttttaat aaaatacttt tagtatggga ggattagtca aatggtagga tttacttgga
    15301 tagattttgc aatactagtg gtttacttat tggcagtttt atttgcagga ttgttatttt
    15361 caaaaaagga aatgaaggga aaagagttct tcaaaggtga tggaactatt ccgtggtggg
    15421 ttacatcagt atcaatattt gccacattat taagtccaat atcattttta tcattagcag
    15481 gaaattctta tggtggtact tggattttgt ggtttgcaca attaggtatg tttatagcaa
    15541 tacctttgac aataaaattt ttcttacctc tgtatagtag gttagaaata gatacagcat
    15601 atgaatactt agaaatgaga tacgaaagta aaggtcttag agtacttgga gctttgatgt
    15661 ttataatata tcaaatagga cgtatgtcta tcataatgta tcttccatca gtggttctag
    15721 ctggtcttac aggaatacct gtaaatgtat tgatagtagt tatgggtgta atagcaataa
    15781 tttattcata tacaggtgga ttaaaggctg tcttgtggac agattttata caaggtatgg
    15841 tattaatagt aggtgttaca gggacattgt tctatttaat agcaagtata aatggtggtt
    15901 ttggaacagt aatggatact cttacaactg gacacaagtt cttagcaagt aatgaagtta
    15961 tgtttgataa aaatatacta tctacaagtg catttataat atttattgga gctggtctta
    16021 atactttttc atcatatgta tcgagtcaag atatagttca aagatttact acaacaactg
    16081 atataaagca acttaataaa atgacatatg gaaatggtgt tttatcaatg ggacttgcaa
    16141 cagtatttta tctaattgga acttgtttat ttatatacta tactcaaaac ccagatttag
    16201 ctcaaacagt tcaacaagac caagtatttg catcatatat agcttatgaa ttaccagtgg
    16261 gtataacagg aatactactt gcagcgatat atgcagcttc tcaatcaact ttatcgacag
    16321 ggcttaattc agttgctaca agttggactt tagatataca aactatcata acaaaagata
    16381 taagtatgga aaagcaaact aagatagcac aatatatatc attaggtgta ggtatacttt
    16441 ctatagctgt tgcaatagta ttagcaaatg gagaaataaa gtcagcatat gaatggttta
    16501 atagttttat ggggctagta ctaggtgttt tagcaggaat atttgtactt ggtgcatttt
    16561 gtaaaaaagc gactaaaatg ggagcatatg ttggattcat agtatcagca attttagttg
    16621 tttatttaaa ataccgtatg ccagaagtaa cttcttgggc atattctcta ataacaatca
    16681 cgacttctgt agtggtaggt acaatagtta gtgcaataga agctaaagtt aaagcaaaag
    16741 tatctttacc aaaagctgag actactgttt attatgaaaa ataatattta aatgataaat
    16801 tgtcttatag agtgattaga taaataattt agttactcta taagattgta taatatactt
    16861 tgtatataaa agtataaaaa tagactttat aattatatag ggagatagtg cttatgatat
    16921 ttggaaatat aaatcatagt aagacatatt caacattgca tgaagattta cttagatgtt
    16981 ttgaatatgc taaagataat tcattaatag attatgaaaa aggtacttat acaatagatg
    17041 gtgatgatat ttttgtgaat attgtagagt ataaaacttg tgaaaaagaa gatagattct
    17101 gggaagctca taagaagtat atagatgttc atttgatgct tgatggatgt gaacgaatag
    17161 atataaattt tatagaaaat ttagaacaaa aaatatatga agaaaagggc gatttcttgc
    17221 ctcttgatgg taaaaataat ggatacatgg aacttagaaa aggtgatttt ttagtttgtt
    17281 atccagaaga tgctcatatg acaggaataa aagttttaga aaaagaaaat atcaaaaaag
    17341 ctatttttaa ggttttggtt agataatgat aatttatata accactaggg ctgaaaatag
    17401 tgatttaact ttcagcgatt agtgagactt cgtaaatttt agttagatta ctattttcag
    17461 ccttagtttt ttatgagaaa attgaaaata attaaaagct taaaattttg taaaggagtt
    17521 aacttatgga aaatatagat gtattcttat taataggaca aagcaatgct agaggattgg
    17581 gaaatccaaa agagagcatc attccaaatg aaaattgttt tgaatatttg agtactgatg
    17641 aaattataaa tatgagatgt gagctagaga cttcagaagg tgatggtact atagcacctg
    17701 cattttcaaa tgaatggaat aagcttactg gaaataaagt ttgttttatt cacaatgcaa
    17761 aagatggttc aagaataaaa aactggaatc atgataataa ctggtttcta aatgacacta
    17821 tagaaaaatt taatacaggc tgtactacct taagtaaaca ttataatata gaaaataaat
    17881 acgttatttg gattcaaggt gaaagtgatg caaaatatgg tagtgatgca ttgtactata
    17941 aagaaagtct aaaaaaaatt gcgtataggc ttaaagaaga atgtatgatt gataaaatgt
    18001 ttgtaagttt aactggttat tggcttggag aagatgaata ttttattaga acaagacgaa
    18061 ttgcagcagc tcaagaatct gcttgtaatg aatgtgatat actgtgtgta ggaagtaaaa
    18121 ttgctatgca atttcatgac agaggattaa ctattgatga tgttcattac actcaagaag
    18181 gtcttaatat gttaggagag gacttatgta aaaatattta taagtatcat ataaccaaag
    18241 aaaaaagtgt attaagtgat actatagact tatcagaagc aaggaaatat atctgtgaat
    18301 tggaaaagat aagtaaaaga tttgtgtaat ataaaatttt aaatagagtt gttaatttat
    18361 aaaaataatt tgtatggaga tatgttatga aaaattacgt ttgtatagat gttggaggta
    18421 cttcaataaa atatggtttt attagagaag atggatttat aattgataaa tcaagtcttg
    18481 atactgaagc aaaggaaaaa ggtggagaag gaatattagc caaaataaaa gatatagtaa
    18541 aaaaatatat agaggaaaat gaaataagtg gtatatgtat atcaactgca ggtatggttg
    18601 acccagttga aggtaaaata ctatttgcat tagaagaatt aattccaaat tataagggta
    18661 tgcaattaaa aaaagaagta gaaaaagagt ttaatataaa atgtgaagtt gaaaatgatg
    18721 ttaattgtgc tggacttggt gaagtgtggc ttggcgcagg tagaggtgca acatcttcta
    18781 tatgtttaac aataggtact ggaattggtg gatgtattat aataaacaat aaactgataa
    18841 atgggtttag taatagcgct ggtgaagtag gatatatgaa tataaatgga agttcttttc
    18901 aagaattggc atctactagc agtctaatta aaaaagtagc aaaaataaaa aatttgaatg
    18961 aaaaggactt aaatggaaaa ataatttttg atttagcaaa gaataatgat gaagattgtt
    19021 taaaagaact cgataatatg ataaaatcat tagcagttgg aatcgcaaat atatgttatg
    19081 taataaatcc agaagtggta attttaggtg gtgggattat ggcacaagaa aagttcctaa
    19141 aaccaaaaat agacaaagca ttaaaggagg ttcttataga gagagtttac aaaaacacta
    19201 atatagaatt tgcaaagaga caaaatgatg caggaatgct tggagcatta tataattttt
    19261 taaacaaaat ataaagaata gaagggtttt taatgagtat tttagaacaa ttggaatcac
    19321 caacttttaa agtcacaaaa tcagataaat tattaatagc ttatataaaa gaaaatatag
    19381 atgatgtatt ttataaacca atatcacaaa tagctaaaga gagcaatatt ggtgaagcaa
    19441 ctataactag gtttgtaaaa aaaatgaatt ttaatggatt acaggatttt aaagttactt
    19501 tagcacaaga aatatcaact atcaataaaa aaaatattat aaataaaaat atacaaaatg
    19561 atgagccagc tttggataca gcaaaaaaac ttttaagttc aaatgtaacc actttagaga
    19621 acacagtaga gataatcaat agtaaggatg tacatgactg tgcaaggctt ataataaatg
    19681 caaagaaagt ttacttcata ggcattggat attctggtat aatagcacag gattcaaact
    19741 ataaatttat gagaataggc cttaattgtg taagttttga tagcagtcat acgatgataa
    19801 tgatgtcttc cataatggaa gaaggagatt taataattgc aatttcacat tctggtgaaa
    19861 cagaagagat tataaaaact gtaaagcttg caagagccaa taatgctaaa attatatcaa
    19921 ttactgaaaa taaaaattct gaattaaagg atatttcaga tgtacactta tcttatgtat
    19981 caggagaaac agtactggaa actggttcaa tatcttctaa attagcacaa tttttcatta
    20041 ttgatttagt atatacacag gtagtaaaag aactttctaa tgaagctatt gaaaggaaaa
    20101 ttaaaactac aaatgctata aaattgttta aaaatgaata aaataagaaa tttgtaatgt
    20161 tttatagagc tctcttaaaa atttaggaat ggagagatat tacatgtcta aattaaaaga
    20221 ttataatatt gataaagtaa taatttttaa taatttaagt actaaaacga agaatcaact
    20281 taaagaaaaa gctaaactaa aaagattaca aaaaaaagaa attttatttt atgaaaaaga
    20341 tagcttagac agtgtttata tacttttaga aggaaaggta tctattttta agataagtga
    20401 aaatggagag agaaaagtaa ttttcatatt aaatagtggg gaagtcataa atgatattac
    20461 atttgactct tctaaaaact ccactgttgg ctgtgaagca tttgaaaatt caattgtagc
    20521 ttattatagt attgaagaat ttttagaaat tatgcaaaat gattttcaac ttacaaagga
    20581 tattatttct tatatggaaa gaagaataag acgtttatat agacagttga aaaattctat
    20641 atccataaaa gtagataaaa aacttgcagc aaaattatat aggttaagta gagaatttgg
    20701 agtttgctgt ggagaatgga ctttactaaa tgtaaatatt acagttacat atttagctga
    20761 tatgcttggc tgtaaaaggg agactgtttc cagaatgata aaggtattgc aaaaagaaga
    20821 acttattaag attgataata aaggttttta tgttaaagaa actgaattat caaggtattt
    20881 caaaaataat taat
//
LOCUS       DAMKPG010000070        20846 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMKPG010000070 DAMKPG010000000
VERSION     DAMKPG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20846
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.073"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="PC621_002589"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="PC621_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF4109736.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="PC621_002590"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="PC621_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF4109737.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="PC621_002591"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="PC621_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF4109738.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="PC621_002592"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="PC621_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF4109739.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="PC621_002593"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="PC621_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF4109740.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="PC621_002594"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="PC621_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF4109741.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="PC621_002595"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="PC621_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF4109742.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="PC621_002596"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="PC621_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF4109743.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="PC621_002597"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="PC621_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF4109744.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="PC621_002598"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="PC621_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF4109745.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC621_002599"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="PC621_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF4109746.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="PC621_002600"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="PC621_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF4109747.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="PC621_002601"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="PC621_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF4109748.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="PC621_002602"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="PC621_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF4109749.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="PC621_002603"
     CDS             6977..7162
                     /locus_tag="PC621_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF4109750.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="PC621_002604"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="PC621_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF4109751.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="PC621_002605"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="PC621_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF4109752.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="PC621_002606"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="PC621_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF4109753.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="PC621_002607"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="PC621_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF4109754.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="PC621_002608"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="PC621_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF4109755.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="PC621_002609"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="PC621_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF4109756.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="PC621_002610"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="PC621_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF4109757.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="PC621_002611"
     CDS             11105..11755
                     /locus_tag="PC621_002611"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF4109758.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="PC621_002612"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="PC621_002612"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF4109759.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="PC621_002613"
     CDS             12524..12793
                     /locus_tag="PC621_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF4109760.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="PC621_002614"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="PC621_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF4109761.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC621_002615"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="PC621_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF4109762.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="PC621_002616"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="PC621_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF4109763.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="PC621_002617"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="PC621_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF4109764.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="PC621_002618"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="PC621_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF4109765.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="PC621_002619"
     CDS             14889..15836
                     /locus_tag="PC621_002619"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF4109766.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="PC621_002620"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="PC621_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF4109767.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="PC621_002621"
     CDS             16318..17151
                     /locus_tag="PC621_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF4109768.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="PC621_002622"
     CDS             17139..18005
                     /locus_tag="PC621_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF4109769.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="PC621_002623"
     CDS             17999..18802
                     /locus_tag="PC621_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF4109770.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="PC621_002624"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="PC621_002624"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF4109771.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="PC621_002625"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="PC621_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF4109772.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="PC621_002626"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="PC621_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF4109773.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8328 a         2450 c         4333 g         5735 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat aattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata ttgtaattta
    20761 aaagtttata taaggttata tattttaaga tattatttaa caatataaat tctgaatata
    20821 cgttatcaaa gtttataata taaact
//
LOCUS       DAMKPG010000071        19948 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMKPG010000071 DAMKPG010000000
VERSION     DAMKPG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.075"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(76..360)
                     /locus_tag="PC621_002627"
     CDS             complement(76..360)
                     /locus_tag="PC621_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109774.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     repeat_region   471..1154
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=597..624
                     /rpt_unit_seq="tttacattccatatagttaatataaaac"
     gene            complement(1985..2857)
                     /locus_tag="PC621_002628"
     CDS             complement(1985..2857)
                     /locus_tag="PC621_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4109775.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(3251..3829)
                     /locus_tag="PC621_002629"
     CDS             complement(3251..3829)
                     /locus_tag="PC621_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF4109776.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3887..4603)
                     /locus_tag="PC621_002630"
     CDS             complement(3887..4603)
                     /locus_tag="PC621_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF4109777.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            4794..5879
                     /locus_tag="PC621_002631"
     CDS             4794..5879
                     /locus_tag="PC621_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF4109778.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(6034..6693)
                     /locus_tag="PC621_002632"
     CDS             complement(6034..6693)
                     /locus_tag="PC621_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109779.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6910..8202)
                     /gene="lysA"
                     /locus_tag="PC621_002633"
     CDS             complement(6910..8202)
                     /gene="lysA"
                     /locus_tag="PC621_002633"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF4109780.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(8220..9413)
                     /locus_tag="PC621_002634"
     CDS             complement(8220..9413)
                     /locus_tag="PC621_002634"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF4109781.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(9774..10391)
                     /locus_tag="PC621_002635"
     CDS             complement(9774..10391)
                     /locus_tag="PC621_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109782.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(10561..12261)
                     /locus_tag="PC621_002636"
     CDS             complement(10561..12261)
                     /locus_tag="PC621_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109783.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(12658..13221)
                     /locus_tag="PC621_002637"
     CDS             complement(12658..13221)
                     /locus_tag="PC621_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109784.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(13380..14093)
                     /locus_tag="PC621_002638"
     CDS             complement(13380..14093)
                     /locus_tag="PC621_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF4109785.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            14385..16049
                     /locus_tag="PC621_002639"
     CDS             14385..16049
                     /locus_tag="PC621_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF4109786.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(16156..17055)
                     /locus_tag="PC621_002640"
     CDS             complement(16156..17055)
                     /locus_tag="PC621_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF4109787.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(17082..17720)
                     /locus_tag="PC621_002641"
     CDS             complement(17082..17720)
                     /locus_tag="PC621_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF4109788.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(18056..19054)
                     /locus_tag="PC621_002642"
     CDS             complement(18056..19054)
                     /locus_tag="PC621_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF4109789.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(19152..19727)
                     /locus_tag="PC621_002643"
     CDS             complement(19152..19727)
                     /locus_tag="PC621_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109790.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         6512 a         3324 c         2049 g         8063 t
ORIGIN      
        1 ctctctctag ctgttacctt atatatttat ctatatcttt agtattaagt tttttaattt
       61 ctactttttt aattatcaat ttctgtatgt agatatacta aaccttccaa cttgagattt
      121 aaataacttt tcaatctcat aaactattct atacacctta ttttcgtact ctaaatcata
      181 atacacattt atttcaccta tactgtttac acttatcgta taatgttctt tttctattat
      241 acatattctt ccaaaatctg acaggtcatt atctatcaat tttaactgtt tttttatttt
      301 tgaaaaatct aatcttggaa atatgtcatt aggataaaaa cttttattat atacaagcat
      361 ttcatccacc ccttatttat tctatttata tcattttata ccctaattaa taatttatta
      421 atcatctttt tccatattat tcaaattctc ttatataaaa acaaaatcaa tttagaatat
      481 gtattattaa atataaagtg actcaatctt tatttaataa gaaaaaacat ttatattaca
      541 tataattaat ataaaactaa tgaagtgggt gtatgaaaga gaaaacacaa ttttaattta
      601 cattccatat agttaatata aaacagttgt ttttattttt tctaaagaga aaaaactaga
      661 ctttacattc catatagtta atataaaact ttctgtatat tttagttgtg ttttaatatt
      721 ataacacttt acattccata tagttaatat aaaacatggc agtaactaag aagaaaacta
      781 tacctttttt attctttaca ttccatatag ttaatataaa acgaggaaat tttatactaa
      841 tgactgatgc tagatttaac tttacattcc atatagttaa tataaaacta ttataaggag
      901 cattatacga ccaaaagcca aagcagcttt acattccata tagttaatat aaaacaagca
      961 acagcaagag tcgcgacaac attaaaaact tcctttacat tccatatagt taatataaaa
     1021 cctttatttt tttctctata tcgagttcgc caaattcact ctttacattc catatagtta
     1081 atataaaaca gtccagcgtt gtgtggtcgt aacacaccaa tcctacttta cattccatat
     1141 agttaatata aaaccccaaa ataaattaag tatttccaat acttatacat atacaattct
     1201 cctaaacttg cagtgagcga gcaatagtgc aattgataac agttatcata cactctcaac
     1261 gctttatatt tcaactatta aaccctactt tatcacaaaa atcgctcact gcaaaacttt
     1321 tatattttta ttatatcata aaactcatac tataaaataa aaatgggata attttacaaa
     1381 ctaaaagcat ctacattctt ataagtattt attatattct ctattccaat gattcctttt
     1441 tctttttatt gcatccttcc tatgtaatcg cactttagct caacaagtct tttattttca
     1501 ttaacaattt tttaaactat tcaaaatcat caaatttttc atcttataca gttaaatcaa
     1561 aatacgtgtt atatctcttt atctatatac ttttcttcct tccatttctt tttctctttc
     1621 tacattttca ctttgttact ttacaatctc aaggttatta tctgctatta aagttttatc
     1681 tttccaggtt tcacaaaaat aaattgaata agtgcaaaaa taacagatac aaaaccataa
     1741 ctattttctt ttctataaaa aaatatatca tcaacaagaa acataataaa tgaaaataac
     1801 tatgaataaa tactctcatt tagaagaaat agacaaagat attccttaaa atttaagcaa
     1861 aatattgttt aaataaaaaa actgactgtc agttaaatgc cagtcagcta ttttatatat
     1921 tttatccaaa catcactata tttgaacatt ttaataatta ataatactta ttaatataca
     1981 aactttactc atacacagaa tatcttttcc aaatttgctc taaatcatca aaatgctgtt
     2041 taatatcttc tttttccttc attccaacag atatagcaag ggcatttgaa atactaagag
     2101 ctggaactag agaatctaca aatgaagcca tattactctt aactagaaga gtgttatctg
     2161 caagtgatgc tactggcgca aataagctat ctgttaatga tattacatgg gctccttttt
     2221 ctttagcata attgacaatt tgataagatt tcttagaata acgagggaaa ctaatagcaa
     2281 ctattacatc ttcttcattt attcttacaa tctgctcaaa agcatctccc atatccattc
     2341 taattatatg aacattgtca agtattatat ctaagtaaaa acctaaatat tgagctacta
     2401 caaaagaact cctcattcca agtatatata tttttctcgc tcttaaaagt ttgttagaag
     2461 cttctttaaa cgctctctcg tcaatttctt ctagtgtaga ccttatatta tctatatcac
     2521 tttttaaaac tttgtttagt attgcaaaat catcggaata atcatgtgcc atttcaactc
     2581 tttgtactgt agttagttta ttttttatga gttcttgaag tgcagcttgt aatttaggat
     2641 atccactata acctaatgca tttgcaaatc ttacaactgt agactcactt actcctacgg
     2701 tttcacccaa tttacatgca gtcataaatg caactttatc ataattattt aaaatgtatt
     2761 gtgctataag tttttgaccc ttactcaatc tcgtatattg agattgtata tttgatatca
     2821 aatgtttact gtccttcatt tcatttgttt cttccatttt tttctccttt tagttatata
     2881 aactattttg ttttattaac actgtctttt tctctaaaaa ttttattttt tatctcaatt
     2941 tcatcttagt ttttatcata gcttcttaaa ctcataaaat tctaactttt ctgtacctta
     3001 tacttcacat agtattttcg attaaattta ttataccatc tttccttgca ttttttaata
     3061 tttatccaat atttctttta aatttttcaa tttttactca tttaaaagtt atctatctta
     3121 ttttgcatat ttagatgtat attttgtttt gagaataagc tgttgtatac ttattattta
     3181 aagtattttt aataggtcac actatatata ttaactctta atagaatctt gtcaagtttt
     3241 ttagtattta ttatgctttt ttcttttcaa ttaaaatcaa ttgtggtggg ttattgactt
     3301 gatttaaaaa cttagattct aaaacattaa attcatcttg atttaaatta ttgacaaggt
     3361 tatatatgca atctttttct tcttgacctc catcatgccc tgtatatatg gcaatgctta
     3421 ttactccatt ttcttttaac atttctaaac ttaactttat agcctttaaa gttgtatctg
     3481 gtttggttat aattgaatgt ttcgctcttg gaagataacc caaattaaat aaaacacatg
     3541 aaacttcttc ttttatatat tgattcattt tttcatgccc atcaaatatc aactttactc
     3601 tatctgtata tccttctttc tctagtttct ttcttgtaga ctttattgct tcttcttgta
     3661 tatcaaaagc atatacaaag cccttttctc caaccttctc agctagatac cttgtatcat
     3721 acccatttcc catagtagca tcaattacta tgtccccttc atttataata ttttctaaat
     3781 aaaatttatt tatatctgta attttagtta aatactttgc atatgccata atactatttc
     3841 tccctactat taactttatt tcattttttc tcaatttatt tttatcttat tttattgatt
     3901 ttaaatattt tatttcatct tctgttaggt atctatattc tccaactttg gtgtctttta
     3961 atactatttt ccccacagaa atccttctta aatttaaaac tggatgattt atagctttgc
     4021 acatttttct tacttgtcta tttctaccct catgtatagt tatttcacac actgaataat
     4081 ttttttcttt attttcttta gttaccctta ttttggcagg tgccgttgtg taatcttcta
     4141 tttttagtcc tgtttcgaat tgtcttatct catcatcact tataattccc ttcacacttg
     4201 ccacatacgt tttatcaatt tcatgctttg gatgggtaag cttatatgtt aaatcgccat
     4261 cattagtaag tattaataaa ccactagttt cataatctaa tctccctatt ggatatactc
     4321 tttcccttac atctttaagt atgtctatta cactgggtct attgaattgg tcttttactg
     4381 tagttatata tccctctggt ttatttaata ctatatatat ataattttca cttggtttta
     4441 cttttttatt atcaaattct actatgtctt tttcttcatc aatattaaaa gataactctt
     4501 ttactacact tccatttacc tttattcttc ctccaagaat tatttcttct gattttcttc
     4561 ttgatgctat tccacaagaa gctatatact tattaattct catacttaat ccttccttat
     4621 cttaaattat gtgtgtttaa tatttttatc aatgttaatt ttgctacatt attatactat
     4681 actagacctt aattaaaaac aagcaaaaaa attatttaat tttcctaaat aattgtaatt
     4741 tcttttcaca tactattaat atgtaatagc tgactaaaca ctattttaag gaagtgatta
     4801 ttattttaaa ggaaaatata aaatattatt taatagtcgt gtttattgga attttatttt
     4861 ttaaatttat taatactcca tcaaacttta tatctagtat tgaaggattc ttaaagtttt
     4921 ttagcccatt tttattagct attttacttg ctttactttt aaatccactt gtgatgcttt
     4981 ttgaggtaaa attcaaagca catagacttt tagccatttt tttatcttat atatttatag
     5041 gttttatttt agcctttgcc attaggctat taattccatc aatagctaat acattaaata
     5101 gattgataaa tgaaatgcct atgtatactg attatataga tagttttata gaaaaaaata
     5161 tgtcaaacat agattttcta aaaactttaa ttcctcatat acaacatagt ttagataatt
     5221 tattaaaaga agcaagtaat tttgttggta aaattcctaa aaacttctta atctatacac
     5281 ttagtatatc ttctatgcta tttaatatga caatgggatt tatactatcc atatacatta
     5341 tatatgacaa agaaaaaatt gcattgggat ttaagagatt tctatattct tctactgcta
     5401 gaaacaaggc agataacatt atagaatttt ttaggactac tcatgatatt ttctatgatt
     5461 atttacttgg tagaatatta gactctctta ttattggtat aattgcattt ttaggatttc
     5521 aatttgttat cagaatagaa aatgctttat ttttagcttc aataatattc ttaggtaata
     5581 taattcccta ttttggtcct tttattggtg caatccctcc aatagccatg actatattat
     5641 atagtccaca aaaaactata tgggtcattg catttctatt tatattgcaa caattagatg
     5701 gaaattttat agaaccaaaa gttatgggta atcaagttgg tataggagct atatgggtaa
     5761 tatctgctat tttggtggga caatctctat ttggtttcat aggagttttt ctttcagtac
     5821 caatagctgc tgttgtaaaa acttatgttg ataaatatat agataatcgt ttacaatagc
     5881 ttatctctac tgtgacttta atttttagtt taaaatttaa ctgttattta ttaaattatt
     5941 tctcaaaaaa tatctcttag taagaaaaaa ttgctaagag atatttttta taatatataa
     6001 tttgttcatt aaatttatgc atatatttta tacttattga ttatttgagt tttgagaatt
     6061 tccaccacta tctgaaccac cagtagtatc ttgtggagtt ggtgctggtg ttggattact
     6121 attatttggc ttttcttgat tttgagaaga actgttatta ttgtcagact tattactatc
     6181 tgatttttta tcattatttt tagaactata tttattattt ttactacttc cactataaga
     6241 agatttcctt gcatctgatt caactctata cctatcttct ccagccgtat cttttgcctt
     6301 tttctttctt tgttcctctg cttcgttttg ttctttttct aatttagtta actcttcttt
     6361 ctttttagtt gcctcttctt taataacaga ggaaccattt ttgattttct ctataagttt
     6421 aagttccttg attgcaccct cataattttt taattcttca aactccgtca tatttgacat
     6481 tctcatagct tgcatataca tagctcttgc attctcatta gtactatctt cttccagtgc
     6541 agaggataat gattccatag cttttttata gttatgtttt tctaaatatg tttttccttg
     6601 ctctactaaa tttatttcat cttttttatt aaatgaacat cctacaagaa atatagataa
     6661 tatcgatatt aataaaatta ctcttcctct cattaaatcc ctccttgtca tatactttaa
     6721 attttatcat aattataaag tttttaaaat attttatatt tattacaaga aatttgttag
     6781 taaaacaaaa aataagcaga tatgctacga acgatacaat aaaccaaaaa ttaatttaga
     6841 gatacatatc ctagcaaatc tgctcataat aaactttaat atttaatttt tattatatac
     6901 ttttccattt tatatctcta aagctaataa gtcctcatag ctttgtctct tacaaattat
     6961 cttatcattt ccatcaaata ctgatactac tgctgctctt ggtattttat tataatttga
     7021 agacattgaa tatccatatg caccagttga tgttgttata agtatatctc cacttttaat
     7081 atccattatg tttatatctc caattaatat atctccactc tcacaacatt ttccagctat
     7141 tgttacatga ttcatagcct catgattaat tttattcaca atactacact cataactagc
     7201 ttggtacaac gaaggtctta cattatctgt catacctcca tctacactta cataaaccct
     7261 aacatctttt atatctttta tagaacctac tgtatacaat gtacttcctg catttgcaac
     7321 tattgatctt cctggttcta ttaccaatgt aggtacttct attccaagct ctttacatga
     7381 agtttctgct ttagtgatta tggtttcaca aaactctttt atagttttag gtttatctcc
     7441 ttcgctataa taaacgccaa ctcctccacc taagtcaatt tctgttagtt gtatgttgaa
     7501 tttttctttt atttctttta ccaagttcat cattatatcc actgtatcta tgtatggttc
     7561 tacatcaaat atttgtgaac ctatatgagc gtgaagacct acaagtttaa tatttttata
     7621 ttcacttaat ttttctattg ctctaaataa atctccattt actaaagcaa atccaaattt
     7681 agaatctatc tgtccagttt ttatataatc atgtgtatga gcttctatac caggagttat
     7741 tctaaagtat atctcttgag ttttaccttt atcttcacat aatgcttcaa gcaaatctaa
     7801 ttcataaaaa ttatccacta caaatttacc tacacctaaa tctaatccca tgtttatttc
     7861 ttctttagtc ttattgtttc catgaaagaa aactttttcc ataggaaaat ttgatttata
     7921 agctgtatat aactcgcctc ctgaaactac atctaaacac atttgttctt catttataag
     7981 attacacatg taaagaggta aaaaagcttt ccctgcataa gcaaccttat tttttccctc
     8041 tctagcctta aaatattcta tatattctct acaattttgt ctaactaaag cttcatcaaa
     8101 tacataaagt ggagtttgat attttttact aagttctaaa caactcactc ctccaatata
     8161 taattcatca ttatttacag tcatagttcc atgaagtttc ataatagcct ccccaaattt
     8221 tatatattaa atacttctgc tattttttct atagctactt gcttatcatc agaatttata
     8281 gcacaagtta tacgaatctc tgatgtagtt ataagtttta tcattatacc attttcattg
     8341 aatatcttaa atactttagc agcaactcca gaagtatttt tcataccaag acctactaaa
     8401 gaaaatttag tgatattatt atcaatcact acatgttctt catctgtata tttagatact
     8461 atctttttac attctcccaa ctcttctttt ggaactgtaa atgatacact tatcttatct
     8521 aaaataggtg cagtttggct tatcatatct acacttatac ctatagttgc tatatcttca
     8581 aataagctag atatattttc agctttaaaa tcttttattg ttatagaact gtcatcatca
     8641 ctagtagcta atcctgttat tactttatcc tctaatttca tgtcttttcc ccctcttata
     8701 tatgttcctt tttctgtacc acatgtacgt ccgacataga tttcaacacc atatttttgt
     8761 gcaagctcta tacttcttga atgcattact tgtgctccta aactcgctaa ctcaagcatt
     8821 tcttcatatt caatctcatc aagtttacta gcctttgaat attttcttgg gtctgtaaaa
     8881 taaattccat caacatctgt atatatctca cattttccat ttaatttaac cgcaagagct
     8941 acagcagacg tatctgaacc acctcttcca agagtggtta catcaccatc ttcgttgatt
     9001 ccctgaaatc cagttactat cacgacattt ccttcatcta aactattttt aattctgttt
     9061 acgtttatat catcaatttg agactttcca tgaagaccac tcgtttttat atttaactga
     9121 taagcattat aacttatagc tttacatcca agtgcattta atgctattga taataaagat
     9181 gctgaaatca tttcaccagt agacattaaa gcatctaatt ccctcttact aggttcatta
     9241 ctcaattcct ttgctaaagt tatatattca tcagtggatt ttcccatggc agacacaact
     9301 attaccattt gtggattttc ttttcttcgt tctattatat tttctgctat tgattttatt
     9361 ttttcagtat ctgctactga acttccacca tatttttgaa caattatact catgctttct
     9421 ccccctcaag ctaaattttg ataaataaaa acgcccaaag gtatactttg gacgtgtaaa
     9481 tttcatagac caagtaacct tgtagctcac cactttaaaa gtgacagcct tatgcatatt
     9541 ctgcataagt ccagaaaatt tgtccgtcaa caaaatttct tcggctatat accctttcat
     9601 caattatcaa tgcttaccag ctcctattga atactattga atacagctac ctctacccta
     9661 ggctcattat ttttaattaa tttattaata tagttttatc atattctata taatattgca
     9721 acaattattt tacaaaatgt ggcaaatcga tagttttctt ttctaaagtt agcttatata
     9781 caattaaata gtttatttaa tcctaaatct ataaaattta tgctatactc attatcatat
     9841 ctctctaaat catttaattc ggatagatta tctggtgtaa ctaccaaaac gtttggtact
     9901 ttaaaaaatg gaattgtaga tttatctatt tttttagaaa gattttcgta tttagaaatg
     9961 tctatttcat tatttaagtc aacatcaact aaaacttgct ttatattacc aaattggtct
    10021 gtatattcaa taaataaatc gtatttcaca tcatctacac taacatttcg gtaaaatctt
    10081 tttacatcac aacctgcagt attgattcta acaactaatt ctgatcctac tagagccttt
    10141 attaaatcat ctcccttaaa aagtttacta ccgtttaaat aatatacata agagaggtct
    10201 ttataattat aataatccac atcacaagga tattttttta gtgtatattc actacacatt
    10261 tttctcaaag tttttttaaa atttttttta ttgtatcttt tttggtctcc ataaattttt
    10321 ctaaattgag actctgtaac acatctaacc ctgctaatta aaagtaatat ctctttttcc
    10381 atattcgtca tagtacattc tccttgaagt attatttgca aagttgtact aatattatat
    10441 taattttatg acattaaatt tacaaagaat taaaataaaa tagctaattt ttatagaatt
    10501 aaattctata aaaattagct atcactatgt taaatactat tatcaatctt tttatttaaa
    10561 ttatacatta ccttgatttt cagaatcttt ggaatctgta ttttcatcag ttcctgtttt
    10621 ttcttttgct tttttatctt caaagtttgc aatatttagg ttatcaaatt ctgacatttc
    10681 tattacacca gattcttctt ttgaatacga tattttgact ttatcaccaa cctgagtcaa
    10741 tggcaattcg ctagatactt ttgaagtagc cctaaatatt atatctttat ttgaatctaa
    10801 agttacataa taattagtgt taccacctcg cacatctggg cttattcttg tcactgtgcc
    10861 ttctaatacc tcatctttta aatcattatc aagttttaaa tcattaccct tagattctaa
    10921 tgcatcctta tagtttctta atgcttcatt cttatcttca cctattccaa gtacattata
    10981 atcttcaaca gatacaaatg caactctctt aacaagacca gccttatctt ttaatgatga
    11041 tacatatgtt ggctgaccca atatattata cattacaggg aacgtcgctt cataattttt
    11101 ttcttgaaca ttaccttcag ctgatttcat tgcagccgtt tcagtagctc ctggttgttt
    11161 atataatcta gcttcttttg ttctagaatc aactaacata aatccaattg tagattcatc
    11221 tccacctgaa gatgttatac ctgtatacca ataagacctg ttatcattgc cataaactaa
    11281 tgatgtacct tctgttggta ctaaaactcc cttttctgat ataacagagt ttaaaaatcc
    11341 attaacataa acaccccagt ctttaatctg gtctgttaca aagctttgtg gttgtattct
    11401 atccacccat ttaggtgtgt ttttaacatc ataaacattt atttttccag tttctgcatc
    11461 aacagtggct attcctgttg catttgctcc tccatagcca attttatgtt catataaact
    11521 tacaacccaa taaggtcttc cttcatcatt tatttcaaga gtgaaatctg tcataccaac
    11581 atttacaata ccatgtatgt ataaatgtct ttgtaaatcc tgatgtaagt aggcttctgg
    11641 ttggtaaact atctttattg gctttccatc tatttcttga actaattgga cgtcttgagg
    11701 attactagct gatactttta cataaccact tgtaccactt agagatgtta tccatttaat
    11761 tatgtctctg tgaacaagag gcgctaccca gtacaactct ccatctacat tttgtatatg
    11821 aaattttcct aatttggata cactccctat tgcaggaact tctccaagct ttttgtcacc
    11881 taatttcatt gccatatctt catctacaag acgaatgtca tttacactga cagggctaat
    11941 gtcatcagta aatttacttt cagttacttt tcccaatagt tctctatatg attttgcatg
    12001 aagtacaggt gtagaagtta taaagggaac tactacaata tacacaagca aagctattgc
    12061 tattgaaaaa ttatatttag ccattttaga tgctctttca tttctatcta acatcatatc
    12121 tagtattcct gctacaacaa agaaaattat tcctactgaa aataaagatg taaaatctaa
    12181 tcttagtaca ggtaatttaa taaatgcata aattattaca attattaatg caactccata
    12241 tgttttaaga tatcgtttca tttaatactc ctctctaaaa aatgttatta ttattattat
    12301 atccatatat ttacaaaaaa taaatattta tcatatttat tatatattaa gatatcttta
    12361 tatatttata gttctgctta ttcaactata tttaacatta tacgtgtatg tataaatact
    12421 ttttatacac tgtttgtgct atccatcccg cacctaaagg agtgggattt ttgcactatt
    12481 ctgtattata tatatccaaa aatattataa aaatacaaat attagcagaa atttaaaaac
    12541 tgaagaaagt attctaacta agatttacaa attcttaatc ctatcgaagt taaactacta
    12601 tcttcagcct aattatctag caatattttt ttatatgaaa aactctaaat caagttttta
    12661 aatgtttgcg atttttgata cataagaatg ttgtctagaa ttatattctg ataataaacc
    12721 ttcatactct aaggttaagc cagataaatt ataaaaatca tttggcgtta cttcaaatgc
    12781 ttgtttattt aaaatgtcta tctcactttt ttctaacatg tcagcaacaa agtttgaaca
    12841 tacatattta tattcagatt gtctctgaat attcattagt aaatatatta atgccattgt
    12901 atcgtaatag taatcttctc tataatcact aattaatttt atatttttat atagattttc
    12961 atattgtaaa ttatctactt ctaacgaata taccctacac acagtgtttt ttcttatagc
    13021 atacaatcct tgatttatat tttcttcaac aaatcctcca ataaatggat tccttggatt
    13081 tagccttcca aaagaataca ttggttttag attttcattt aatgatattg acacatgtgt
    13141 atacagcttt ttacttatat tccttattaa gtaagataat atagttcctg tatatgttgt
    13201 tacaatatag actttattca tcatttcccc ccaatctagt aactaaatga tattatacac
    13261 ttacaattta atatcataag atattctaac ataataaatg tggttttaca aattttgatt
    13321 ttaaggtact ttaatattat ataatataat aaaaatataa atataagttt tctatatttt
    13381 taaccaacag atgttttctt caaattaatc agatgattta gtatatcttg aacctttcca
    13441 gagtccttta aatatcctaa tgccccattt atttgtgatt ttaaatctat ttctacttcc
    13501 atatccaaat caacatttcc tttttttata gatatatttt taagtgttat tgggtaaaga
    13561 taactttttt ccaaagtaat cttatttcca ttaacctcaa atggaacttt tttattgtag
    13621 ttacttaata ttttttctag cattttatca cttagtttaa attttccaat tttcacattc
    13681 tcaagactta caactaagtt tccatccttt aaagtaggtc tcaattcaag ttcatattga
    13741 ctatttacca aaccaaatac cttatatggt cctgctacct ttattttacc atttttcatt
    13801 tcaacaaaat tattttcaag ttcattaatt ccatgcttct gcatcaatgt atatattaaa
    13861 tttttaaact cttcctctga aacagatatt tttccaacca ttctaagtgg attttttact
    13921 acctccatag aatttatata tgagcctttt gataaatatt cttcatctat gattttatta
    13981 ttttgtgaac taacatcata tctttctttt ggtgtcaaga tatatacaac aattcctgca
    14041 agtactaaaa caataacaat taaacttaac aaaatctttg caacctttcc cataatcgtc
    14101 tcctttacaa atctgtcatc agatttaaaa taaatttaga taactaactt cacataatat
    14161 tcttaacaat atacatacta taatacagtc ttttaaaaat taattcaacc aatctaatgt
    14221 tttgtaaata aactgtaaaa acatgttttt agtataaaag ttaatatttt gcataatact
    14281 cttaattaca cctttatttt tttctaatct tggattaaaa ttaatattat gcaatacagt
    14341 gtagtgttct gtattataat caaattaaat aggaggtata gaatatgtca aatgaaacga
    14401 actcatctaa ctttataaag aatattatta taaacgactt agaaacagga aaacatgaca
    14461 gtatcataac tcgttttcca cctgagccaa acggatattt acatattggt catgcaaaaa
    14521 gtatatgtct taattttgga ttagctaagg agtttaatgg aaaagctaat ttaagatttg
    14581 atgatacaaa cccattaaaa gaggatgttg aatatgtaga gtccataaaa gaagatgtaa
    14641 agtggttagg atttgattgg aacgagttaa attttgcatc taactacttt gatgaaatgt
    14701 ataagagagc tttaatacta ataaaaaagg gtaaagctta tgtatgtgat ttgacacaag
    14761 aggaaatgag agaatatcgt ggtactctaa ctgaaccagg aaaagaaagt cctcatagaa
    14821 acagaactat tgaagaaaac cttgatttat ttgaaagaat gaaaaatggt gaatttaaag
    14881 atggtgaaaa aacattaaga gccaaaatag atatgtcttc tccaaatata aaccttagag
    14941 acccaattat atatagaata tctcattcaa cacaccataa tacaggagat aaatggtgca
    15001 tatatcctat gtatgctttt gctcatccaa ttgaagatgc aattgaagga ataactcact
    15061 ctatatgtac tttagagttt gaagaccaaa gacctcttta tgattggttt gttaaagaat
    15121 gtgagatgga aaatatacct agacaaatag aatttgctag acttaatata aataatactg
    15181 tcatgagtaa aaggaaatta aaacaacttg tagatgaggg tattgttgat ggttgggatg
    15241 accctcgtgt tcctacaata tccggaatca gacgtaaggg atatactgca gaagcattgc
    15301 gcaatttctg tagtgaaata ggggtatcaa aagttaactc tacggtagat agtcaaatgc
    15361 ttgattattt cctaagagaa aacttacaac caaaggctcc tcttactatg ggagttttaa
    15421 gacctttaaa acttattata actaattacc cagaagataa gattgagatg cttgaaatag
    15481 agaataatgc taaagatgaa tctcaaggaa agagattagt tcctttctct agagaattgt
    15541 atatagaaca agatgatttt atggaagaac ctgttaagaa atattttaga tttttcccag
    15601 gaaatgaagt ccgtttaaaa ggtgcttatt ttgtaaaatg tactgatgta attaaggatg
    15661 aaaatggaaa tgtagttgaa atacatggta cttatgaccc tgaaactaag agtggctctg
    15721 gctttacagg acgtaaggta aaatctacaa tacattgggt tgatgctaaa tcagctatac
    15781 catgtgaatt tagattattt gaaccattaa ttcttgatga tatacctgaa aatgaaggta
    15841 aacatttctt agaacaaata aatcctaatt cattggaaat attacaaggt tttgttgaac
    15901 ctacacagat aaaagattct aaaccttttg ataaattcca atttgttaga aatggattct
    15961 ttagtataga taataaatat acaactgacg aaaaatttgt atttaataga atagttcctc
    16021 taaaaagttc ttttaaacca ggtaaataat cttacaatca aaaaatgaag acttaaaata
    16081 taaattactt ctactataaa taaacttagt tagaagtaat tttttatttg agtcttcatc
    16141 atttacttta aatgattaac ttttttcact acagattccc ataacttcta ttccatcttt
    16201 tatgcattct atgttaagag gaaaatctgg tcctttttct ccgtcaatat ctgttataag
    16261 tgaatcttca cattctatat gcaccttttt agttttaaag tatagtattt catttccatt
    16321 atattgatct aaataatcac ctctaagcac acttatcaaa actggtattg attttggtat
    16381 tggcattcct ttaaaaataa ttacatccaa atatccatca tctatttcag ccttatatgc
    16441 tagatttata tttcctgctg ttttgccatt aaaaatcaac ataagataca tatctccatc
    16501 ataatacatt tcctcagaag atacttttat cttaaatttc ctcatataca atgcttcttc
    16561 tattcccttt atataataag aaattcttcc aaatgaattt tttaagtctg tgtttatcct
    16621 ttgtgataca tctgtaaaca taccagcact tgcaacattt ataaagtact tattattgac
    16681 tttccctatg tctattttct ttggttttga atttatgatt tcatttatag cttcttttat
    16741 atcaaatgaa atttgaagag catttgaaaa atcatttgca gttcctgtag gcaaaattcc
    16801 aattggaatt tttatgtcta attctttcat tgcatttact actaaatcta cagtaccatc
    16861 tccaccagct atcaatatgt gttcataaga actatcaact tccttaaatg cattctttac
    16921 aggttcttta atagttaatc tatagggtat caataaataa tttttttctt gatataattc
    16981 tatgatagtg tctaaatttg ataatatctt tttttcacct gaattaggat tgtatataag
    17041 ttttactttt ttcattattt ctccttactc attttattct tttagtatcc aaaatcttca
    17101 tttaaaaaaa tcacatgtat tctttctggt gttaattgtc tttttataag tgtataacta
    17161 cagcatattc ttcctggact tgaacagtct atacatatac cagtagtctt acatccagta
    17221 tttgtactta atcttttaca attggctgga gcagctattg tttttgttct ttttatagct
    17281 tcatctacat ctttaactat tttatttact cctgctataa ctattacttt atcaggtcca
    17341 tatatcatag cagcaactct attcccattc ccatctaaat tgtaaagttc tccatcctct
    17401 gttatcgcat tagtacttgt aaaataagca tctgcagaaa atgactttct atatatttct
    17461 ttcatttctt ctggtttgat atcttttgca tatctatcaa gaaaattata tttttctctt
    17521 ataaaatcaa tcactcctgt ttcaaataag gtcatagaac ctccacatcc tactagagaa
    17581 cctactttaa ctatttcttc aattttttta agaagctctt ctttgttact tactaaatac
    17641 cctttcatat tattgttttc cagagatttt attgtttttt ctattctttt ttcattaagc
    17701 caatttaaat tttgatccat tttaacttcc cccttatttt taatatgtac ttaattattt
    17761 ataaacttta ttgtctttat aagtttattt tagtacatct aaaaaaaata caatataaaa
    17821 cttaaatagt tttatataat tttttccaaa ataacttact caatcaaaaa aggaaagtag
    17881 atgtactatt ctactttccc tatattgtat gtagtattta ttgcctaaaa tctatatatt
    17941 aattatatgt ttcatattat ataatatcac agttaattaa aatgtaatac tatcttcttt
    18001 ttaaacatat aaattgtatt attaaattta atatatccaa cttcaataaa tcttattaat
    18061 ttgatgaaga agatgctata gctgcacttg ttgctgccat tacacatgca atttgaatag
    18121 caacaacaat atttaaaaat atattattag ttatagagcc tatattattc tcatttcgta
    18181 tcaaatctgc ataaattgaa gctacaatag cagaactaat cattactcta ttactttttc
    18241 ctaaagacca atttccaaag cctttttctt ctttaagttt atttgaaaca ttttttatct
    18301 cttctattat ttcatcttct ctataatcta ataatgaaat tgctcctatt attggatatc
    18361 cataataatt cattttacaa tcattttctt taaatgcttt tttaagttca atagtctttt
    18421 tacatttaag catagttcta tcatcactaa ataaaagaat ttgggataat gcttgtaaat
    18481 catttccttt ataaaaacca ttttcattta gaaattcata acatttttct atgtattcta
    18541 aatcttcatc taaatttttt gaatgaattg caataagtgc tatattacaa tagtcatcac
    18601 tagaagttag aaatggatgt tttttcctca tgtaatcata tattattttc atttttgata
    18661 tacatgtaag ataatccatt ttctctctat tttcataaac tattgatgct gtaagtggta
    18721 aatatgtatc gccccaaaac ttttcacttt taagttcttt gtatatatct aaaatcatct
    18781 taaaactact ttctggattt gattcaaacg aaagtagtgt tgccatataa aacatattat
    18841 atcctctaaa attagagaat aatccagtat tttttttaat aagctctatt cgttcttcca
    18901 ctaatctagg acttattctc ttgttcttaa gagtaaatgc aagtgcacaa ctatgttgta
    18961 tcaatcccat ttgccaagaa cctttaactt catttaatgc tttgtaattt tctattaata
    19021 agtttacttt tccatttata aacgattgtt ccatattgcc ctcctagtaa tatatctcat
    19081 tgtactaata gttttatatt tattatatat atattaaata ctacaatcaa tacatattac
    19141 ttcaaagaac atcattcttc aacaataatg tagccttttt tgtaatgtct tcccatatta
    19201 ttgactctat catagtactc tgattcactt tcattactcc atttgggcac aaatacatct
    19261 attgcattag aattactaaa atacttacta gaccccctat ccataacagt ttttaatttt
    19321 ccatctacaa taatcttagt tccaaaactc aagtgattgt ttgctacaaa accatcttct
    19381 aaatcatctc ctgtagcagt tatcgctcca tatccatttt cttgattaag acttgtgtaa
    19441 taactaactt caaaagttat aggtactaac ttctttattt taatacctaa ctgattttgt
    19501 agtttctcct tttgtacttt caacttatac tcatcactat ttttataatc ttccatacct
    19561 tgattatact tggaattaaa gcttttacct gactttacta tagtatctat atttactata
    19621 gattctttac aatgatctgc atctattttt gtttctccat ttaccaataa tggagttaaa
    19681 catacactac atacaacaca tgtaatcaat gcttctttaa gcttcataaa ttcccccctt
    19741 attattttga tatattagca tatttaatat tatatgtcta tatttagatt taaataccac
    19801 acccatttgc tttaagaagt tgtcttatat ttaattcacc ttaaacactt ataaatcctc
    19861 tatcttagaa tcatatagaa taaagctttt atcgttaaat attttttatt aatcttttaa
    19921 aatctaaagc tactttattt aattttct
//
LOCUS       DAMKPG010000072        19850 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMKPG010000072 DAMKPG010000000
VERSION     DAMKPG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.076"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            195..1346
                     /locus_tag="PC621_002644"
     CDS             195..1346
                     /locus_tag="PC621_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF4109791.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1392..2567
                     /gene="thiI"
                     /locus_tag="PC621_002645"
     CDS             1392..2567
                     /gene="thiI"
                     /locus_tag="PC621_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF4109792.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2723..3526
                     /locus_tag="PC621_002646"
     CDS             2723..3526
                     /locus_tag="PC621_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF4109793.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3769..4410
                     /locus_tag="PC621_002647"
     CDS             3769..4410
                     /locus_tag="PC621_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF4109794.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            5823..7493
                     /locus_tag="PC621_002648"
     CDS             5823..7493
                     /locus_tag="PC621_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF4109795.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(7615..8349)
                     /gene="pflA"
                     /locus_tag="PC621_002649"
     CDS             complement(7615..8349)
                     /gene="pflA"
                     /locus_tag="PC621_002649"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF4109796.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(8509..10740)
                     /gene="pflB"
                     /locus_tag="PC621_002650"
     CDS             complement(8509..10740)
                     /gene="pflB"
                     /locus_tag="PC621_002650"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF4109797.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            11033..12094
                     /locus_tag="PC621_002651"
     CDS             11033..12094
                     /locus_tag="PC621_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF4109798.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            12185..13798
                     /locus_tag="PC621_002652"
     CDS             12185..13798
                     /locus_tag="PC621_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4109799.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            14073..16058
                     /locus_tag="PC621_002653"
     CDS             14073..16058
                     /locus_tag="PC621_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF4109800.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            16063..16497
                     /locus_tag="PC621_002654"
     CDS             16063..16497
                     /locus_tag="PC621_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF4109801.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            16526..17086
                     /gene="srlA"
                     /locus_tag="PC621_002655"
     CDS             16526..17086
                     /gene="srlA"
                     /locus_tag="PC621_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF4109802.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            17150..17518
                     /locus_tag="PC621_002656"
     CDS             17150..17518
                     /locus_tag="PC621_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF4109803.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            17543..18109
                     /locus_tag="PC621_002657"
     CDS             17543..18109
                     /locus_tag="PC621_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF4109804.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            18150..18515
                     /locus_tag="PC621_002658"
     CDS             18150..18515
                     /locus_tag="PC621_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF4109805.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            18616..19389
                     /gene="srlD"
                     /locus_tag="PC621_002659"
     CDS             18616..19389
                     /gene="srlD"
                     /locus_tag="PC621_002659"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF4109806.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         7715 a         2222 c         3439 g         6474 t
ORIGIN      
        1 ttttgtaagg tatagtataa gtatattata ttttactata aaaatttgta ataaagtgaa
       61 aaaactagct tttcatgaaa taatatttta taatttaaga ttgtcttttt aataaagatt
      121 aacaaaaaaa atactataat tagtggtata atagttagag ttgaagattt taaataaagg
      181 taggtgtaat taagttggaa atatacttag acaatagcgc aactacaaaa ccttatcaag
      241 aagttataga taaaatggtt tatgcactta atacggaata tggaaatcct tcttctgtcc
      301 atagaaaagg tgtagaagtt gaaaaagcta taaaagaagt aagacaagat atagctaaat
      361 ctctaggagc gaaagaaaag gagatttatt ttacatcagg tggtacagag tgtaataata
      421 ctataataag aggtattact agtttaaata aaaaaagaaa aaatcacata atatctacta
      481 acatagaaca tccttctgtt ttaaatacat taaaagattt agaagaagat ggttttgaag
      541 taacttattt agaggtcgga aaagatggga aaataaatat agaggattta aaaaatgcga
      601 taaagtcaac tacatgctta gtaagcatga tgcatgttaa caatgaaata ggaactatac
      661 agccaatagg tgaagttgga aagtatttga agggtttaaa agaaaaaatt tatttccatg
      721 ttgatgctat tcagtcatat ggaaaaatta attttagacc atctaaatac aatatagatt
      781 ttatgagtgt aagtgcacat aaatttcatg gtccaaaggg aattggattt atgtatataa
      841 aagaaaataa tcgccttaag cctatgttaa caggtggtgg tcaggaaatt ggaattaggt
      901 cgggaacaga aaatgtacct ggaatatatg gtcttggaga agctgttaga atactaaaca
      961 aagatttaga tgttgttatt tcgaaagtaa ataacttaaa aaatatatta aaaaatgaaa
     1021 taattgataa tatagaagat ataaaaatca attctccaga agatggagta tgtcatatat
     1081 taaatgtatc atttagaggg acaaaaggag aagttttact tcattaccta gagcaaaaag
     1141 gtatttatgt atcaactggc tctgcatgtt cttctaaaaa gaaaggtagt tacgtattaa
     1201 atgctatagg tcttacaaat gaagaaataa atggaacaat aagatttagt ttatctgata
     1261 tgaatacaga agaagaaatg ttagaagctg tgaaagtttt gaaagaatca atttgtgact
     1321 taaggtcaat aatgaaaaga aaataaagaa tactgtatag atagtgataa taaattagtt
     1381 taagaggtga aatggtgtat aatatattaa tagtaaagta tggagaaatt ggagtaaaag
     1441 gaaagaatag atatatattt gagaataggc ttataagaaa tataagaaat atgttaaaac
     1501 caattggaag atttaatgta tataaagagt atggaagaat atatgttgat ttagaagact
     1561 atgattatga agaagtaatt gaagaagtta gaaaagtatt tgggatagtg ggtgtgtgcc
     1621 ctggagtaag agctaaaaaa gattatgata cattaaaaga aatagcttta aaaatgttag
     1681 aagaaaaaat agaggctgga tataaaactt ttaaggtaga gtcaagaaga ggagataagt
     1741 ctttcgggtt gacttctcaa gaaatgagta tggatatagg tggatactta ttatcaaagg
     1801 taggagatag gattaatgtt gatgtaagaa atccagaagt taaaataaag tgcgaataca
     1861 gagaatttca tactatggtc tatagtgata ctatacctgg atatggtgga ttgccacttg
     1921 gaacgaatgg tagagctatg tctcttttat caggaggtat agatagtcca gtagctacat
     1981 ggatggttgc aaaaagaggt atggaggtag aggcagttca cttccatagt tatccattta
     2041 caagtgaaag gtcacaggaa aaagttaagg atttagctaa aatactggca aaatactgtg
     2101 gtagagtaag acttcataaa gttaacatat tagaaataca aaaagcaatt ggtgagaatt
     2161 gtaatgaaga agaagctact attctttcaa gaagatttat gatgaggata gcacagagac
     2221 tttctgaaaa aagacattgt gatgctttaa ttacaggtga aagtattggg caggttgcat
     2281 ctcaaactat acaagggctt acttgtacta atgccgttgt agacttacca gtgtttagac
     2341 cacttatagc aatggataaa tcagatatag ttgatatagc caaaaaaata gggacttttg
     2401 aaacatctat agtgcctgaa gaagattgtt gtagtgtatt ttctcctaga aagcctgtca
     2461 ctaaacctag attagagaaa atagaaaaat ctgaaacagc acttgatatt gaaaaattag
     2521 tacaggatgc tattgacaaa atagaggtag aggatattga gttttagcat acaagttatt
     2581 taattactaa attgaatatt gagtatatta gtagttctgt aagtatatta gtagttctgt
     2641 tgaaaggatt tatgtagaca atttattaat atatagtatt taaaaataag tactataatt
     2701 ttatttaatg ggaatgatta ttatgaataa aaagggtttt acattaattg aattgttggt
     2761 agttatatct ataataggaa ttttagttat agtagctgtt ccagcgttat ttagaaatat
     2821 agaaaaaagt aaggcagtta catgtctttc taatagagaa aatataaaga ctcaaattgt
     2881 tattgcaatg gctgaggaat caagtaaaga caagaatgaa gtcataaaag aggtattaga
     2941 aaacaaagat ggtaagtact ttgaaacaga accaaagtgt aagtcaggtg gaatatattc
     3001 agcaacgttt gatgatggtt atgatggaat aactggaata gaaagcattg caaaagtgta
     3061 tgttacttgt acaaaacatc cagatggtat tgaaatggct agggatatac atcaaagtat
     3121 gaaagatttg attgcatcat ttgcacaaga cccttctata ataccaggag cttcaaaggg
     3181 caatgatgat tttagaaaat atttattaga caataaatat aaaaatgggt ggcctacaat
     3241 tccagatgaa tttaaggcaa aatatggatt aagtaaggat acactatata tacaaccata
     3301 tgcatataat cctactaaat ctgatgctac tgtagttgta tttgcaaata ataagactgg
     3361 aggtaattgg tatacttccc tagtttacga ttatgatgaa ggtagatggt ataaaggtaa
     3421 aaatggtatt tctgttgcag gtaggtcatg ggatgttgac acagatagtg ttaagtctgt
     3481 aaaaacagag attcattcta aagagggatg gggtccttta aattaatata tcatgtttta
     3541 ttaatctggt attgattaaa ctatagaaaa gagcagtttc tatagatgac gaataccaga
     3601 ttttcattta tatgtataat taagatatat taaaagtatt tatattttta agtcttcaca
     3661 tacaagctaa agataattat aataatattg taaagaaatt taattataag attaattttc
     3721 tgaagattta gtatttgtac taagaaatac ataaaaaggg gaatagatat gaataataat
     3781 tttcaagaca accaaacaat aaatttaata caaagtcgta gaagtatacg aaaatttaca
     3841 acagagcaaa ttagtgatga gcaagtaaac acattattac actgtgcatt tgcagcacct
     3901 tcaggatgta ataaacagcc ttggcacata actgttgtgc aagaccagaa acttttaaaa
     3961 gagattagtg atgatacttt atctaggatt catgaagtga gtaatgttga gataaataaa
     4021 aactttaagt tattttatgg tgctccaacc gttttattta tttcttatga tgaaagtagt
     4081 tcatgggctc catatgatat aggaatactt acagggaata taacaacagc tgcacaagca
     4141 ctagggcttg gaagttgtat aataggaatg gttagaggtt tatttacacc tgttgaacaa
     4201 ggggatattg aaggtcttgt gagtgtattg gataaagaag atgtaaagga atcagagtca
     4261 ataaagatga agtttgatac aaataaaaaa tacagagaac tacttgatat accagaaggg
     4321 tattctgtac catttggtat tgctgtaggt attccagatg gaaatttacc aaatgctaga
     4381 gaggtagttt acaaagtttc cagagtttag gataaagtat tgcaagaacc aatcagttac
     4441 ttgaagaatt aaaccagtaa tatacaaata aattgatgga agaggaagct atttaaaaag
     4501 tagtttagat ttttcattgt aaaataatta aataaagaat aaggtgataa gtattttatt
     4561 tattatctta ttctttttta tttttgtttt gtagaatgat aaaaataagg attaagtgta
     4621 ataaaataga aaacaagtat gttaatttta agtatatgct ttaaaattta tccaagttaa
     4681 aaatttaaga tatcttttca gtataatgga aaaaatgtca aaatattgaa atagggaaaa
     4741 aatagtgata taataaaaaa aatagagaaa aaaagtaacc cttagttgta aaaaagttac
     4801 tatttattga aaatttagat acttttctaa aaattatcta tgttaatagt taaatttatg
     4861 aacatttttt gtttttatgt taggatattt cttagaaatg aaatacttct taatttgatt
     4921 tgatatgtat atttttatag ctattttatt ataaaaaaaa agaacccttt tacaacaaat
     4981 ggaaactatg tacgtaataa ttaaaataaa aaatattatt ttatatttat aaacattgac
     5041 attctaattc tgtagtgtaa tcgtattttt tattaattct tataaaatca atgaaaataa
     5101 ggatatccat tttaaagtac ataaatttaa aaatccagaa aaatatactt aaattatgta
     5161 agcaaaagag gggttgaatc ggctgaaaat gtaatactag taaatagtac atcaatccta
     5221 gataccctgt aagcaactct attttcatgt actaaaaatg tacctatttt attaactgaa
     5281 aagaataaat taaatgtaag tacataataa gagataaaaa attaaaagca aaaaatattt
     5341 atataaataa atataaaaaa tgatataatc cctattaaaa aatttataga tgatgaagaa
     5401 atatcaaaat cttatattat aggaagaggc acttctttgc caaaatatat agaaaagcaa
     5461 taccaaatcc aaagagaata ggctgagaaa aaataaaaca aattcaagag taatagaaaa
     5521 gctttataga ataaagaact gagaactctt tatataataa aaaatggact tttaagagaa
     5581 aatgatttaa taaatgattt aacatggtgt tttagcaggt gaaaataaat caccagtaat
     5641 gattgtagaa aaattacttt atgaatctca aaaaaactta gttaagagta agagatttga
     5701 taagataact aaagttgggg aaatgtgttt gatgagataa aaaaattagt aaaatagtat
     5761 aatctaaata agaatatttt gttgattaaa ctacactaat agtaatattg tagggggaaa
     5821 agatgagaga agaatcgaac tctttaagtt tagaccatat agaaattttc tttgaacttt
     5881 tatctcaaag tacagaagat tatatatttt tttgggatat aaataggaat aaatttaaaa
     5941 tttcttcagc aatttttgat gaatttaact taagtaaaga gatagaatct gatttagtca
     6001 attgttggag taaaattgtt tatccggatg atgttcaaat atggaaagat gatatacaag
     6061 aattactaca tggaaaaaaa ggtgagcaca atttagaata taggttgatt aataaatatg
     6121 aagaaatagt atggatttct tgtagaggta aagtttatgt gtcggatgac ccaaaaacta
     6181 tatttctagt cgggagaata aagaatattg gtgaaaaaaa taaatttgat tctatcacag
     6241 gaacgtggaa tagagaacaa tttgaacata gaatgaatta tcttataaaa gagaaaattt
     6301 ataaaaatgg tgctatgttt atcatggata ttgataattt taaaaatata aatgaaaaat
     6361 atggacattc ttatggtgac aaagtattgc gtgcaattgc tactgaagta ttagaatatc
     6421 ttccaaaaga tgtaagattg tataggttag atggagatga atttgcattt ttttacccta
     6481 tgtgtactaa agaaactata gagaaaatat atgaaaagat acagatgtat acaaatactc
     6541 agcatgaaat tgaatcaaat aagtactatt gcacagtgac tgcaggagtt gctatgtatc
     6601 cagaagatgg agataattat ttagatttat ttaaacatgc tgatattgca ttggatatag
     6661 caaaaattag tggtaaaaat agaattaagt ttttttcaca agagctctat gaaaataaac
     6721 taaaggtaat ttcaatgcaa caaaaactaa gagagtgcgt tgaaaataat tttaatgact
     6781 ttgagctgtt ttttcaaccc caggtaaatg ctgttacaaa agaggtaata ggggcagagg
     6841 tacttttaag atggcattcg agtacttatg gagaagtatc tcctgttgaa tttataccaa
     6901 tactagaaca gagtaattta attataccag ttgggaagtg gattataaaa gaagctgtaa
     6961 aacagtgtaa agaatggcat aaaataaatc cagactttaa aatatctgta aatgtatcgt
     7021 atattcagtt aaaagaagat ttttttagag attttatagt agaatgttta gtagagtatc
     7081 aattaagacc tgaattttta attttggaat taacagagaa ttgttggata ccagatataa
     7141 atttactaaa tgataaattt attagtttaa agggtatagg agtatatatt gcaatagatg
     7201 actttggtac agggtattca tctttaaatt accttaaaga actgtctgtt aatataataa
     7261 aaattgaaag aagttttgtt aagaatatta catataatag ttatgaatat acttttcttg
     7321 aatatattat taaattagct catattataa atttaaaagt ttgtgttgag ggaatagagt
     7381 cttatgaaga gtatgatata gtaaaaagct taggtgtaga tattattcag ggttttttat
     7441 ttggaagacc tgtaagtgcc tctgaatttt ataaatttca actatcaaag tgatgtacat
     7501 aaaatgagat atatagtata taaaaatagg gcaaagtgga agttttgaag tttattaaaa
     7561 atttacactt taccctattt tgttatgtct tctataaagg attaatatac tacactaact
     7621 attatatact ttcactccaa atttagagat tatttcacta gctttttgga catcttcctt
     7681 agtggcatca ggaacatctt ttagttcata atcgatacct aaactttccc atttgtgtat
     7741 tcccatagaa tggtatggta gcaattcaaa tctttctaca tttttcaatg tagctacaaa
     7801 ttgacctagt ttttctaaat tatctatatc atcagtaatg ccaggaacta aaacatgtct
     7861 tatccataca ggaatatttc ttgattctaa gtgtctagca agttttaatg ccttatccat
     7921 accaacacca gtcaaatcaa tagatttttc ctcaaccata tgttttaaat caagtaaaac
     7981 caaatcagta ttatctaaaa ctgggtctat agtttctata tctacgaatc cagatgtatc
     8041 caagcaagtg tgtatatcat tttcttttgc ttttttaaat aattcactta aaaattctgg
     8101 ttgaagagtg gattcaccac cagaggcagt tatacctcca ccagaaaact tcataaaaga
     8161 agtatactta agagcatccg ttattatttc atcaactgtg tattcttttc ctgatttaat
     8221 atcccatgta tccctgttat gacagtattt gcatctaaga ggacacccct gaaaaaataa
     8281 tatatatctt attcctggtc catctacagt accaaacgtt tctatagaat gtatctttcc
     8341 cttaatcata gagttcacct cacaatatat tttaataacc ttatcaatag ttataatatt
     8401 tgtctaaaaa aggccatctt ataccagaaa taattcaaat ataagatagc cttttatttt
     8461 tattttttac acagtagtaa tcttagttaa agtataaaaa tttaaagact aagccatttt
     8521 accgtggaat gttctgttta taacatctaa ttgttgctct ttagttaatt ttatgaagtt
     8581 aactgcatat ccagatactc ttatagttaa ttgtggatat tcttctggat gttccatagc
     8641 atcttctaaa gtagctctat caaatacatt tacatttaag tgatgagcat tttgagagaa
     8701 gtatccatcc atcattgcag aaagattgtt tattctttca gtcatatctt ttcctaaagc
     8761 agcaggtact atagagaaag tattagatat accatcttga gcatgttcat atggtaattt
     8821 agcaactgaa gataatgaag ctaaagcacc attagtatct ctaccatgca ttgggttagc
     8881 accaggagca aatggagcac cagctcttct accatctggt gtattaccag ttttcttacc
     8941 ataaacaaca tttgaagtta ttgtaagtat agattgagtt ggataagaat ttctataagt
     9001 tttatttttt cttattttat tcataaagct ttcaactaac tctacagcta tactatcaac
     9061 tctatcatca ttgtttccgt attttggata atctccttct atttcaaaat caacagctat
     9121 tccttgttca tttctaatag ttttaacctt agcatgtttt atagcagata atgagtctgc
     9181 acaaactgat aacccagcca taccacaagc catagttcta aatatatctc tatcatgtaa
     9241 tgccatttct aatgcttcat aagaatattt atcatgcata taatgtatta catttaaagt
     9301 atttacatat aatgttgcta accaatcagt gaatggttca aatttactca taacttcatc
     9361 ataatctaag tactcagaag ttataggttc aaatcttggt ccaacttgaa caccagattt
     9421 ctcatctaca ccaccattta tagcgtataa taaagtttta gctaagttaa ctctagctcc
     9481 aaagaactgc atttgtttac caattctcat tgcagataca caacaagcta taccatagtc
     9541 atctccccag tatgctctca ttaaatcatc attttcatac tgtactgaac ttgtatctat
     9601 agatacttta gaacagaaat ctttgaatcc ttgaggtaat ttagttgacc atagaactgt
     9661 taagtttggc tctggagaag gtcctaatgt atatagagta tttaacattc taaatgagtt
     9721 tttagtaact aatgttctac catctattcc catacctcct atacactcag ttacccaagt
     9781 tgggtctcca gagaataggt tattgtaatc aggagttctt aggaacttaa ccattcttaa
     9841 tttcataaca aaatggtcca tatactcttg taattcttct tctgttacga ttccagcttt
     9901 taaatctctt tcaaagtata tatctaagaa agtagatgtt ctacctaaag acattgcagc
     9961 accattttgg tctttaacag cacctaaata accaaagtat aaccattgta cagcttcttt
    10021 tgagttagtt gctggttttg atatatcaaa tccataagat tcagccattt ttttaagttc
    10081 atttaaagct gatatttgct cagttatttc ttctcttaat ctgataactt cctcatccat
    10141 gcaagaaact tctaatgatt tcttttgctc atttttatct tctattaaag catcaacacc
    10201 gtatagagca actcttctgt agtcaccaat tattctacct ctaccataag catctggaag
    10261 acctgttatt ataccagatt ttctagcagc tctcatttct ggagtataaa catcaaaaac
    10321 tccttggttg tgtgtttttc tgtatttagt aaatatatct tttatttctg ggtctaattc
    10381 atatccaaaa gcttcgcatg aattttcaac catctttata ccaccatatg gcatgacagc
    10441 tctttttaaa ggggcatccg tttgtaaccc aactattgtt tctaattctt tatctaaata
    10501 tcctggagca tatgcagcta ttccagaaac agtttttgta tcaacatcta aagtaccacc
    10561 attttctctt tcttttttaa ataggaccat tgcttcgtcc cataattttt tagtattttc
    10621 agtggcacca gctaagaaag agtcatttcc ctcatatgga gaatagttta attgtataaa
    10681 ctctctaaca tttatttctt tagtccatct tcctgtttta aatccttgcc atgcattcat
    10741 taagtatttc ctccttagat ttaaatgata aataatacta ttaaaattat tttttatata
    10801 cttaatttat tttatatatt tttttaaaaa tatattttct aatataataa tatcatatta
    10861 ttcgtatcct gtatacataa aaaataaaaa attgtcgctt taaagtataa aacagattat
    10921 atgttaataa tgtggtgaaa ttatacccat atataaataa aataaaacaa ataaatagct
    10981 aattaaggag aaaatatttt ttattttttg tttaggataa ataaatttta ttatggaaaa
    11041 aagaaaaagc atacatcttc acaaattgaa tatttattta atagggggag gtgatagatt
    11101 gtttatcaca gtggtattat ttttagtagg tttcctatta attactaaag gcgcagatat
    11161 ctttataaat tgcactgtag agataggtag aaaaacgaat atatcagaaa ttatacttgg
    11221 agctactata gttagttttg caacaacatt acctgaattt acagtgtctc tacttgcttc
    11281 tattgatggt catactacta tgagtttagg aaatgcagtt ggttctataa tatgtaatac
    11341 aggattagca ttagggttgg ttgtttttat aagtccattt aatgtagata aaaaaatgtt
    11401 tttttctaag tctttactat taatagtatc cgttatagtt ttaattttat tgagtttaga
    11461 tggagttata acaagaggtg attcattatt acttataata atattaatct tttatatggt
    11521 taataattat agaagtgtcg ttggaaaatc tgatactaaa aatagaaata ttaaaaataa
    11581 cactattaaa gacatatcta ctaaaaaagg gaaaaattat agaggttttt ccgtattaga
    11641 aattgtaaaa atactattac tttttgctac aggattgata atgatgataa taggttcaca
    11701 aatccttata gagagtggag taataatagc tagtttttta aatatacctc agggtatagt
    11761 aagtttaact ataatagcac ttggaacttc tctgccagag atagtttcgt cgattactgc
    11821 tataagaaaa aatcatcatg aaatatcagt aggaaatatt ttgggtgcaa acatattgaa
    11881 tatagtatca gtaatagctg tatcagcaat accaaataac ataccaatac tatctcaaaa
    11941 tagacaattg gatataccat tcatgatatt attattatta atagtaataa taccaacact
    12001 taaatctaat agacttagta gaatacaagg tatattgatg ttattcacat attttctgta
    12061 catatcaatt ttgtatttca tgtatattat ttaactatac tgtttaaaat attaattgca
    12121 gaatatccta aaaaagtata taatggatag gttaagatta attgataacg gagaggaata
    12181 atatatgaat ataacaaagt ttgaagattt accaataagt gagggaataa aaaaagctat
    12241 agctgaaatg gggtttgaag aaccatctcc tatacaagca cagtctatac ctgcgatttt
    12301 gtcaggtaaa gacgttatag gacaagctca aactggtacg ggaaaaacag ctgcttttag
    12361 tatccctata ctagaaacaa tagacccaaa taatagaagt ttacaagctg tagtgctttg
    12421 tccaacaaga gaactggcaa tacaagtatc aacagaaata agaaagttag ctaagtattc
    12481 gcacggaata aagacattac ctatatatgg tggccagcca atagatagac aaataaaatc
    12541 tctaaagagt ggtgtgcaag ttgtaatagg tacaccagga cgtacaatag accatatcaa
    12601 tcgtaaaaca ttaaagatgg ataatgtaaa aatgattata ttagatgaag cagatgaaat
    12661 gctagatatg ggatttagag aagatataga aatgatttta agtaagattc cagaagaaag
    12721 acaaacaact ttcttttcag ctactatgcc aagaggaatt ttggaattaa ctaagagata
    12781 tcaaaaagac ccagagcata taaaagttgt tagaaaagaa cttacagttt caaatacaaa
    12841 gcaatactat atagaaacta ggtcatcaaa taaattagaa gtattatgta gattagtaga
    12901 tgtatatgac cctaaactat ctgttgtttt ctgtaatact aagagaaaag cagatgaatt
    12961 ggtaggagat ttacaagcaa gaggatattt tgcagatgca ttacatggtg acttaaaaca
    13021 gactcaaaga gatatagtaa tggataagtt tagaaatggt acaatagata tacttgttgc
    13081 tactgatgtt gcagctagag gtatagatgt tgatgatgtt gaatgtgtat tcaactatga
    13141 cttaccacaa gatgaagagt attacgttca tagaattggt agaactggtc gtgctggtag
    13201 agaaggtatg tcatttactt ttgtatttgg aaaagaaatg agaaagatga aagatataga
    13261 aagatatact aaatctaaac taataaagca taatatacca acaataactg atgttgaaga
    13321 gaaaaaagta ggtactttct ttgcacaagt taaacaaact atagaagaag gtcacttaac
    13381 taaacaatta caatggttag aaggtttctg taatgatgaa gattatgcaa tggttgatat
    13441 agctgctgca cttgtaaaac tatctctagg tgaagaaatg aaagaagaaa taatagaaga
    13501 aaaacctaga agagagcgtg gagatagaaa aggtggtact ggagctaaag atggtatgat
    13561 tagattattt atcaatattg gtagaaatca aagagtacaa gcaaaagata tagttggtgc
    13621 tatagctgga gaagttggaa taccaggtaa agtagtaggt actatagata tatatgataa
    13681 gtatactttt gttgaaatac ctaaaaaaga tgcaaaaaca gtaattgaaa agatgaaaga
    13741 tattaagata aaaggtaata agattaatat agaaaaagca aataagaaga aaaaataacg
    13801 tatagtatac aaaactataa ttagaaaata tataaaataa tatattggat atttaaaaag
    13861 cctgatgtgt tttcaaaaca gatagtagat aatctgtaag agttaacagc ataaggcttt
    13921 tcttgtttta tatgatatta tgattttcca gatcgttgtg aaaaatgtat attagttcct
    13981 ttttttcaaa tctgatttga aaaagagaat attaattatc tatatagttt agattataat
    14041 aaaaaatgta agataacaaa ggggagaaaa agatgtcaat gacttatatg gaccctaggg
    14101 tttttttgat tttagaaatg gtgttagaag aagacacaaa tactctagaa caattttcaa
    14161 atgttttggg tgttagtact aggactatca gaaactatat aaaccagatt aatcgagaat
    14221 taaaaggtgg tattgctaaa atagttaaaa atgaaaaagg tacatatagt ataagaattg
    14281 aggatgaaag taaattaatt gagattgtaa attttaatag aggaaagcta tcaaatttta
    14341 taaatttaaa ttcacctgat gaaagaataa attatgttct tgatatttta actatgacca
    14401 acaatccaat taccatagat gatttggctg aagagatatg tataggaaga acaacattaa
    14461 taaaggattt aaaaaaagca gacagaattt tagctgaata tgatttagaa ttaaaaagga
    14521 agccaaatat aggtatgatt ttagttggtg aagaaatgga tataagatta ttaatccttg
    14581 atagattgta tgaaaattat attgatgtct tggaaaacgt taatcagata aatttagtta
    14641 aagattatga tattgaatgt cttagagatg aattaaaaaa gttgtttaaa aaagaagaac
    14701 tctacataac agaacaggtc ttgagagatg ttgaaagata tataatcatg tctgtcttaa
    14761 gaaatctaaa tgggtacaaa tttgaaaaga tagataagag atttgaagtg ataagatgtt
    14821 cagatgaata tactttagga ttaaagctca aagctttatt ggaaaagata tttaatatag
    14881 ttttaaatga aaaagaaacc atatttttga caatgccatt aataggtaga aaagctccta
    14941 gtacaaaatt agcacttagc agtataaaga taaatgatag cgtaaaggaa gttgtagatg
    15001 aagtaactag ttttttatta gagacctcag ggattgattt taaagaagat aaaaagctta
    15061 tagaaaattt ggaatatcac ttgtattttg cacttaatag aatgagattc aacataaggg
    15121 taaaaaatcc tcttttacaa gaaataaagg aaagatatac atttccatat agtgttgcaa
    15181 agatagctgc aatgataatt gaggaaaagt ttgacttgaa aatatgtgac cacgaaatag
    15241 gatatatagc attacatttt ggaagttatt ttgaaaaaaa ttcaagaaaa gttctaaatg
    15301 taaatagggt agctgttgtt tgtggaacag gtcttgggac tgcacaactt attagaataa
    15361 agataggaaa aatacttgga gaaagtgtag aagtaaatac tttttctgat atagaagcta
    15421 aagctaattt agatttatta aagtattatg atttagtatt tacaacagta gatttcaaaa
    15481 cagacttaga tgttccaatt tttagagtaa atgcgttatt taatgaagaa agtataaaga
    15541 aagagataga aaatgcaata cttttgaaaa atagtataaa tcttaagtct ataaaaaaga
    15601 ttgaaaaaaa tatccctttt ataggtaaat taattgaaga ggataagttc tttatattga
    15661 ataagagtac gtttatggaa aatttagaag atatgttaga aaaattagtt cataaaggaa
    15721 ttattgatat agagttcaaa aaaaaggtat taaaaagaga aaagaaatct tcaacagcat
    15781 tagataatta tatagcattg cctcactcag ttaatactaa tggggaaaat ctttatctgt
    15841 catttggaat attagaaaaa ccagtggttt gggatacaaa ggaaattaga ataataatat
    15901 tgatgataat tccagataag aatgtagata gtacagacct gataataaaa agttatgaag
    15961 aagtacttga agtagggaga gataaaaaaa tgatagaaga acttgctaaa gtaaaatcat
    16021 ttgatgaatt ttataaaata attaccaaga ggaggtaata atatggaccc agtaattttt
    16081 attatagcat cacttattac agtgtattta ttaaatattt ttcttggata cttacagctt
    16141 aaagatttta ataaaaatta tattgaacta aaaagaaaag gaagagtggc aataggcaga
    16201 aaaaaaggaa gaatttcatc aggtacaatt gtgttgatac ttataaatga tgatggtgtg
    16261 attgtcgaaa ctagaaaaat gcaaggtgtt acagtattgg caagagtaaa gtcatttgaa
    16321 ggtttggtag gaaagagctt agagtacata gaaaaatcag atttaactgt ttacaataaa
    16381 ctacttaaaa aagcaatact agatgcagtc aaacagtaca taacatttga gaaaaataaa
    16441 tgtgaagata caaataatct gagtactgat gaagagtgta ggatggaagt agtttaaaaa
    16501 aatatattta taaggaggag ttaatatgac gcaatttttt caagtattag caagtggtgc
    16561 agatagtttt atgaatttat ttagggcagg tgggcaacaa tttgtaggtt ttgtaacaga
    16621 tatagtacca cttttagtag cattattagt tacaatgaat gcaattatca actttattgg
    16681 tacagataga gtcgagagac ttgcaaaaaa atgttctgca aatatattta ctagatattt
    16741 gatattacca gtccttggta catttgtatt tgcaaaccca atgacactat cactaggaag
    16801 attcttgcca gaaaagttta aacctagtta ctttgcagca gcaagctttt catgccatac
    16861 aatgaatgga ttattcccac atatcaatcc aggtgaacta tttatatatc taggaattgc
    16921 aaatggtata acaactttgg gattctcaac agcagattta gcagttagat atttgttagt
    16981 gggtatagtt gcaaacttta ttaaaggtgt agttactgac tatacaacaa aatttgtaga
    17041 gaaacaacaa ggaatagcat taaattcaga tataaagatt ggataattac tttaaggtat
    17101 aaataaattt ttcaaataaa aagatagtgt atagaggagg ataaattata tggcatttaa
    17161 caaagttaaa atagtaaaag gttctggagg atggggtgga ccattgataa tagaaccaac
    17221 agaaaagaag aataaggtag tatatgtgac tgggggagca aagccagaaa ctgcagtaaa
    17281 aatagctgaa cttacaggat gtgagttaat agatggattt tcacagggtg ttaaagatga
    17341 tgaaatcgca tgtgtaataa taaattgtgg tggaacattg agatgtggta tatatcctca
    17401 aaagaaaata ccgactataa atataatgaa aacaggaaaa agtggaccat tagcaatgtt
    17461 tataaaagaa gatatatatg tttcaggggt aaaaccaaca aatgtagagc agttataatt
    17521 gaggtagtag gagggaagaa atatggctta tgatactaaa aagaagataa gtgagcagag
    17581 tcaaggaatt gttgcaaaaa taggtatagc actaggaaat gtgacaagta ctctttttca
    17641 agctggtagg gatactatag atacattaat acatactata ctgcctttta tggctttcgt
    17701 tgcagtccta ataggtataa taaatcaatc aggtatagga aattgggtag ccaatttact
    17761 tactccatta atcggaaatg ttggaggttt agttgtctta tcaataatat gttcatttcc
    17821 gttgttgtct ccgtttcttg gaccaggagc tgttatagct cagatagtgg gggtattggt
    17881 tggtacagaa ataggaaaag gaaatattcc accaagttta gcattaccag cattgtttgc
    17941 aataaactca caagcagctt gtgactttat accagtaggt cttggtcttg cagaagctga
    18001 acctgataca gttcaagtag gagttccgtc agttttatat tctagatttt taacaggtgc
    18061 accaactgtt ctattagcat ggttagcaag ctttgggtta tataaataga agaatacttt
    18121 ataaataatt agagagaggt tcagttgata tggaattaat atacagtact caaataaata
    18181 aaataggaga taatgcaaat gaatttttac aacataatat gtttgtaact ttcaaagata
    18241 atgctcctca agagttagaa aattactgtt atatacattc tgaaaataat ttagtaaaag
    18301 aaatacttac gaatgatgtg ttatcaataa ataatgttga ttacaaaatt acttctgttg
    18361 gtgagttagt aaaccaaaat ctttcagaat taggtcatat aacatttaag tttacaggtg
    18421 aaaaagatgg agcaattgga gggaccttat atcttgaaga aaaagaaata gcacctattg
    18481 gcgtaggtac tatactaaaa ataattagaa aataaaattt aaaacgaatc taatcatatt
    18541 ataagtaaat tatagataaa agtcaattta actaatcaag ttaactttta atgtgataaa
    18601 aaaggggaaa tgaaaatgag aaaagtggct ttagttgttg gaggaggaag aagtttaggt
    18661 gaatatttat ctaaacattt agctaaagta ggttatgatg ttgctattgc agatttaaat
    18721 gaagacaacg caaaaaaagt agctgaagaa atagaaactg aatttggttg taaatctgta
    18781 ggatatggtt gtaatgccat agatgaaaga caggttataa gcactgttaa agctattgaa
    18841 aatgattttg atagaataga tttattagtg tacaatgcag gagtggctgt aagtaaaaaa
    18901 ataactgatt ttggatacaa agattttaaa tttattgtag acgtaaattt aaatgggtac
    18961 tttctatttg caagagaagt atctaaagtc atgatagcaa aaggtattga gggtagaatc
    19021 atacagataa attcaaaatc aggtagagtg ggaagcaagc ataattcagg atattcagca
    19081 ggtaaatttg ctggagtagg acttactcaa agtttagcgc tagatttggc agaacataag
    19141 ataactgtaa atgcactgat gctcggtaat ttacttgatt ctgatatgtt tgaaagttta
    19201 attcctcaat atgcagtaaa attgggaata cctgaatcag aagtcaagca agtttatata
    19261 gataaagtac ctttaaaaag agggtgtaga tttatagatg ttgcaaatat gcttacattt
    19321 tatgcttcaa aggaagctga atactgtact ggtcaatcta taaatattac tggtggacaa
    19381 gttatgtaag gtttttatgg agtgtctcaa gatgaactct ttagttcatg aggcactttt
    19441 tttgtataaa attaaatata ttttcatcat ttaaacaatg aaaaagaggt ttatctttat
    19501 tttgagatag cctcattttt gtaattaaga aacatagtgt ttaagtaaac atataagatt
    19561 gttagttttt taaaagagaa ttttttaata atattatatt tatgaagtat ttttaaactt
    19621 aatttgatta aaaaatagct acgcacactt gtgactttag tcatgagtta gtagctaaat
    19681 tagtcagcat atagggaaac ttatatgtag agataggata gaacctatcc aatactactt
    19741 gaattgctaa aaacccctaa agctaaccaa actaaaacat aagggtgaaa taaacctaag
    19801 tgtgaaagtt acgaaagtag aaaaaattgg ttagatggta taaggttaaa
//
LOCUS       DAMKPG010000073        19132 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMKPG010000073 DAMKPG010000000
VERSION     DAMKPG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.077"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            207..785
                     /locus_tag="PC621_002660"
     CDS             207..785
                     /locus_tag="PC621_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4109807.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            824..1402
                     /locus_tag="PC621_002661"
     CDS             824..1402
                     /locus_tag="PC621_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4109808.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1488..2117
                     /locus_tag="PC621_002662"
     CDS             1488..2117
                     /locus_tag="PC621_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4109809.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2107..4866
                     /locus_tag="PC621_002663"
     CDS             2107..4866
                     /locus_tag="PC621_002663"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF4109810.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4953..6515
                     /locus_tag="PC621_002664"
     CDS             4953..6515
                     /locus_tag="PC621_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF4109811.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6516..7652
                     /locus_tag="PC621_002665"
     CDS             6516..7652
                     /locus_tag="PC621_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF4109812.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7716..8792
                     /locus_tag="PC621_002666"
     CDS             7716..8792
                     /locus_tag="PC621_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF4109813.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8770..10008
                     /locus_tag="PC621_002667"
     CDS             8770..10008
                     /locus_tag="PC621_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF4109814.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10062..11150
                     /locus_tag="PC621_002668"
     CDS             10062..11150
                     /locus_tag="PC621_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF4109815.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11178..11969
                     /locus_tag="PC621_002669"
     CDS             11178..11969
                     /locus_tag="PC621_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF4109816.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(12084..12638)
                     /locus_tag="PC621_002670"
     CDS             complement(12084..12638)
                     /locus_tag="PC621_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF4109817.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12833..14035
                     /locus_tag="PC621_002671"
     CDS             12833..14035
                     /locus_tag="PC621_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF4109818.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14092..15072
                     /locus_tag="PC621_002672"
     CDS             14092..15072
                     /locus_tag="PC621_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF4109819.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15319..16272
                     /locus_tag="PC621_002673"
     CDS             15319..16272
                     /locus_tag="PC621_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF4109820.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16262..17218
                     /locus_tag="PC621_002674"
     CDS             16262..17218
                     /locus_tag="PC621_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF4109821.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17215..17970
                     /locus_tag="PC621_002675"
     CDS             17215..17970
                     /locus_tag="PC621_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4109822.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18062..19027
                     /locus_tag="PC621_002676"
     CDS             18062..19027
                     /locus_tag="PC621_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4109823.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7564 a         1938 c         3421 g         6209 t
ORIGIN      
        1 atattaaaca tagcttttat acatttaata aagtatgtaa aatctatgta aaaagtaaga
       61 aatataaaca tttttttaat tatattttaa aaatatcaag caagttttct tcacaaatga
      121 ataaaaaatg ttataatatg attaaagaaa aagataaata attagcaaga tttgtttagc
      181 taaaattaac aagggagcga taaattatgg gtataacatt atcaaaaggc caaaaggttg
      241 atttaactaa aggaaatcca ggtcttaaaa atattttagt aggtttagga tgggatacta
      301 acaagtatga tggtggattt gactttgatt tagatacagc agcattttta acaggagcaa
      361 gtggaagtgt tactaatgat ggagattttg tattttacaa caatttaaaa catacatctg
      421 gagcagttga gcatctagga gataacagaa ctggcgaagg tgatggagat gatgaacaaa
      481 tagttgttga tttatctaag attcctggag aaataagcaa gatttctttt actgttacaa
      541 ttcatgatgc gacagaaaga agacaaaatt ttggacaagt aagtaattct tatataagaa
      601 tagtagataa agacacaaat gaagagttaa taaagtatga attaggagaa gattttagta
      661 tagaaacagc tatagtagta gctgaaatat ataagcacaa tggagaatgg aagtttaatg
      721 cactaggttc tggttttgaa ggtggtcttg ctgcattatg tggaaacttt ggaataaact
      781 tataatttca actataacaa taaactaaag ggggatattt tccatggcta tagtattaaa
      841 aaaaggacaa aaaatcgatt taactaaggg aaatccaggt cttaaaaata taaaacttgg
      901 actaggatgg gatacaaatt catttgatag tggctatgat tatgatttag atgtaagtgt
      961 tttcatggtt ggagaatctc aaagagttga aaaagatgaa gattttatat tctacaataa
     1021 cttaagacat ccatctggag cagttgaaca cttaggtgat aatagaactg gtgaaggtga
     1081 tggagatgat gaggaaatat tagtgaattt agagacaatg ccaaagcata tacaaaagat
     1141 agctgtaaca gttacaatat atgaggctgc tgaaagaaga caaaattttg gacaagtaag
     1201 caactcatat ataagagtat taaattctga aaatgatgaa gaaatattaa gatatgactt
     1261 aggtgaagaa tttagtatag aaactgcaat tacagtatgt gagatatata agtataatgg
     1321 agaatggaag tttagtgcag ttggagcagg atttgaaggt ggcttagaag cactttgtag
     1381 aaactatggc ttaaatgttt aatttaagca atataatata ttaaatataa ataacctata
     1441 caaataatgt ataggttata ttatataaaa ttttgtggag gtttagtatg tcgattaatt
     1501 tatcaaaagg tgataaaata gatttaaaaa aatctaaccc aggactgagt aacatacttg
     1561 taggtctagg atgggaccct gtgcaacaat ctggaggagg tttttttaaa tcgttatttg
     1621 gtggaggaca agctgatata gattgtgatg catctgtatt tatgcttaat caagaaggta
     1681 aattaagtgg aataaaagat ttaatttatt ttggaaatct aaaaagtgcc tgtaaatcag
     1741 ttttgcatac aggagacaat ctaactggtg agggtacagg tgatgatgaa caaatcctag
     1801 ttaatttgga taaagttcca tcaaatattc ataaattatt gtttgtagta aatatataca
     1861 attgtgtaga tagaaaacaa cactttggaa tgatagaaaa tgcctatata agagttgaag
     1921 accaaggtaa taagaaagag atagcaaaat acaatctatc tgataattat tctgaaaaaa
     1981 ctactcttat agtaggagct atatatagaa aagatggaag ttggcaattt aaagcaattg
     2041 gtgaaggaac aaaagatgcg ggtcttaaag aagtaatgca aaatttagac aggattgagt
     2101 gtgcatatgg aatttaataa tgatatcaaa tattataatg tagataaaga agaagttaca
     2161 aaagacttat ctgttaatcc tgaaaaaggt ttatcagaat cagaagtcaa gactagaaga
     2221 gaaaaatatg gtttgaatga atttactcca aaagaagagg gtagtttttg ggatgattta
     2281 aaagaaagtt taagtgaacc aatgatagtt atcttgatta ttgcagcagt agtaagtgca
     2341 gtaattgggg aaactcatga tgcaataggg attgtaggtg ctatagcaat aggtatagca
     2401 ataggtatga ttacagaagg tcgttctaag aaagcagcag aggctctttc taagttgact
     2461 gaaaacatag aagtaaaagt attgagagat ggggaagtac atcaaatttc taagagtgaa
     2521 ttggtaccag gtgatatagt atatattgaa actggtgaca tggtacctgc tgatggtaga
     2581 cttatagaaa gtataaacct taaaattaga gaagatatgt taacaggaga atctgatgac
     2641 gtatctaaaa agtgtgatgt agtagtttct atggaagaga tagaatctaa aggagcagta
     2701 gtagtacagg agccaatacc tgcaaaacag ataaatatgg tgtttggtgg tacacttgta
     2761 gcttatggta gaggagctct agttgtaact tcaattggag ataatagtga aatgggtaaa
     2821 attgctcaaa acttatcaga aacagatgag caaactccac ttcagataaa acttggaaat
     2881 ttaggtggtt tgatagccaa agtttctagt gcagtagcag gattactatt tatatttatg
     2941 gttttccaaa tgataagtaa gaatgtatta aatgtagata tgtctggaat attaccgttt
     3001 ttagaatcaa tagacccagt taaaacagca tttacagtat gtgttgcgtt gatagttgca
     3061 gcagtgccag aaggtcttcc aactatgata aatatgacac ttgcaataac aatgcaaaaa
     3121 atggctaaaa taaatgcatt ggttaccaaa aaagaagctt gtgaaacgat aggttctgtt
     3181 tctgtaatat gttcagataa aactggtaca ttaactgaaa atagaatgac tgtagaagtt
     3241 gcatatgttg atggcagata tattgaaagt tcagaagagg aaataaatag ttattttgaa
     3301 gaaaactgta tgataaactc tacagctgat gtagagcaca atgatggtga tattaagtat
     3361 ttaggaagtg ctactgaatg tgcattgctt ttatattaca aaaacgtaga ttatagacaa
     3421 gttagaaaaa attctaatat agtagctcaa aatcctttta cttcagattc aaagagaatg
     3481 acttctgtta taggtcaaga taatcatcat gtactattat caaaaggagc accagaagtc
     3541 ttacttgaat tatgttcaca tattcaaaga ggaaaagaca tagttccatt aactgaaaat
     3601 ataaaacatg agattttaga agaaataaag aaattgcaaa taaaatcaat gagaacttta
     3661 gggtttgcat ataaagaaat ctcacaagct gaagaagaag cagcagtaac tgctgaaagt
     3721 gatgctatga atgtaagtgc aatggaaaat aatttagtat ttagtggttt tgttggtata
     3781 agagacccac ttagaaaaga tgttatagag tctgtaaata cagcaaataa agctggtgtt
     3841 tctgtaaaaa tgcttacagg agataatata aatacggcaa gagctatagg tgaagagtta
     3901 ggcctattaa aaaataatat gagagtggtt gaagcttcat atatagatac tttagatgat
     3961 gaagaattaa aacaagagat acaaagtatt tcaatcgtgg caagaagtaa accagatagt
     4021 aaaatgagaa tagtttcagc tctgcaaaag agcggggatg ttgttgcagt aacaggtgat
     4081 ggtataaatg atgctccagc actttcaaaa gcagatgttg gtatagcaat gggtatatct
     4141 ggtacagagg tatctaaaaa cgcagcagac ataattttaa ctgatgatag ttttagtacc
     4201 attgtaaaag gtattaaatg gggtagagga atttatgata acttccaaag attcgttcaa
     4261 ttccaattaa ctgtaaatgt aattgcattt ttagtggcta taatatctca agttatggga
     4321 caagagatgc catttactac aatacaatta ttatgggtaa acataattat ggatggacca
     4381 ccagcactag cattaggttt agagccagtt agagaccatg tgttaaatag aaaacctgtt
     4441 gatagaaaag ccaatataat ttctaagtca atggtgtata caatagtatt aaatgcattt
     4501 tatataacag caatactaat gttacaatct acatttaatt tcttaggagt tgatgaagct
     4561 cataagggaa cagtaatgtt tgcgttattt gcttttagcg cactgtttaa tgcatttaac
     4621 tgtagagagt ttaatgcaga tagtatcttc cctaatttta cacataacaa attagcacta
     4681 caaataatag tgttgacagg aattgcacaa gtaatattta ctcagctatt ccaagatttc
     4741 tttaactctg tagcaatgga ttttatgaca tgggtaaaag ttttatgtgt agcagcaagt
     4801 gtaattgtgg ttaatgaagt tgtaaaatgt ataataagac tggtaaaacc aaaaaagact
     4861 gcttaaaaca aattttttag aagaaaacag actgactcaa aatatgaatt ttgggtcagt
     4921 tctgaataaa catttaaatt gggaggatag ttatgggtga ctcaaaggta tttgaaaaaa
     4981 tattctccag ccaatcagat agaggaaatt acactccttc aaagggatat ctaagttatt
     5041 ttatttcgta tattggttta gaagatgaag ttttgtacaa tctcgagata tttaagacaa
     5101 aacaaaatat tgatagcaaa aaagatattg ctttatttac tgatgttata gcaaatccta
     5161 gtgattttga tattataaat tattttaaaa gtggacttca aaaatataga actagcatgg
     5221 aagatgtaga tataaatatt ttgggatttg aagagattga ttataaaata aagcaagcaa
     5281 tggatagagt gttaaaagaa gaggaaaaag aatttactaa tgatagagtt aaacaaaatt
     5341 ttatagtgaa aataatggca tggataaaaa tttatatcgg tgctttagat ataaataaaa
     5401 atgaagctcc aaaagtaata ttttatggag atataaaaaa acatgaagtt tatttactct
     5461 taatattata cttggcaggt tttgatgttt tatatcttaa tccaaattca aagagtaata
     5521 tagatatttt aaaatcagaa agatacaata tagagtttga agaagccaac ataatagaag
     5581 aaaaaatttc ttttgaagaa agggttattt taggggaaaa aatagataaa agttctgtaa
     5641 aaaaagcttt tactgttggg gcagaagcat caaagcgtat aagtgaggaa ttgttaaatg
     5701 atgcaggatt tataaaacct tggcaactcc aagatagaaa aataaaaaat ctacttctaa
     5761 gttcaactgt tgatgaaata tcaatatact ggaatcaacc acttaaatta agaccaggat
     5821 ttaagtttaa tgatgctatt gtagaagctc caaattttct ttctaagatt aatggaatat
     5881 ataatgataa gaatgaatat attaagtttt tagatttatt aagagattcg gaaagttcaa
     5941 catttattga atttaatgga gatgtagata gattttcaaa agcttttacg agagaagctt
     6001 ttagtttatc ctttttattg gattcaaaag gtgtaataga taaaaactct gttttaaata
     6061 ataaagatta ttccatatca acactagcac ttaatcaaca aattatgatt ttagagaaag
     6121 tggaagagtt gttagaagga agtatgtttt tgaatggttt aagtggagaa gacaagataa
     6181 aaggtctatt tacagtactt cacatggata agaaatttgt tcatatgatg aacaatttcg
     6241 attactcact tataaaccca aaattgatta tatacatgta caagagtatt gtatttgata
     6301 aagaaatagt ttttcttatg cttttactta gtaagattgg atttgatatt atcatattat
     6361 gtccaggagg agaaaacaat atagagaatg ttataaataa ccaattgatt gatatacaca
     6421 gattggataa aatggtatat gaccttaaat taaattcgct tgaaaatgat atacctttac
     6481 ttaaaaaaat ttttggaaag aggagacgat tttagatggg gataaatata aatagttttt
     6541 caaaagataa gaactctgta gaggttactg agaatttgcc tacagcagtt caagaagaaa
     6601 acttcgatat aatggaatat acaaataata aaaaaaatga actgagaaag tctaaggaag
     6661 ttgaggcatt gacatccctt atagaagtag aaaatccaga tacgatttta cagtttggta
     6721 ggaaagcatc tgaaggagta gcaagagttt ctgatagcct tttaaataca ataaaattaa
     6781 atagaaatga agaaaattct aaaatgctgg ttcatttaac taaaataatg gataagtttg
     6841 atttagacga ttttcaagaa actaaggaac caaatttcgt tcaaaaactt ttcaaaaaag
     6901 ctaataatgc cattgaaatg atgtttcaaa agtatgaaac attgggagga gaagttgaga
     6961 aaattcaaat tgagcttgag caatacgaaa gagatatagc actttctaat aagcaaatag
     7021 gtgctatgtt aaatgaaaac tttgagtttt ataatgaact tcaaaaatat attgttgctg
     7081 gagaaatggc aatagaagaa atggataatg aaatactacc tttctttaaa caaaaatcag
     7141 agactagtgg agaccaaatg gatgttgtca attatcaaga actattaaaa gtttatgata
     7201 tgttaaatca aagagtctat gatttaagaa tagcagaaaa tatagctatc caaacaatac
     7261 ctatgcttag aggtatgcaa cataataatt atggtttaat tagaaaaata aatagtgcat
     7321 ttgtcgtaac tttaccagta tttaaacaat gtttatctca agctatatta cttaaaaagc
     7381 aagaattaca agcaaaatct ctaaaagcac ttgatgacaa aactaatgaa ttattgctta
     7441 gaaatgcaca aaatgtaagt actcaaagtg cacaaatagc tagaatggct ggaacaagtt
     7501 ctgttcaaat agaaacattg gaaaaaatgt acaatactat caagtctggt atagatgaaa
     7561 ctatgagaat agaagaaaac aatagagctt taattaagga taatacaaag agattggaag
     7621 aattaaatac gactataatt tataataagt agtattatat taagggctaa tttgtataaa
     7681 gttggccctt tctatgtata tgaggaggct agacattgaa atatttcgat tatatctgca
     7741 aagatgattt ggagagaatt tttttgaagg agccagaaga ctttagtgct aagactgaaa
     7801 aagatgtgtt gaaatatgca ctgggcgctt ttttatacgt tccagctact caatataata
     7861 tgatttataa gagtattata ggggatgtta agggagttag accattagct atttgtttag
     7921 aagatgctgt tggagtaaat ggagagttag aggctataga aaatcttaga ttaattttaa
     7981 aaaatattag caatgaaagt ataactaata aagatggaat accactaatt tttgtaagaa
     8041 taaaagatgt ggagcaactg ttgagaatta aagagattat aattaaaaat agtcattcta
     8101 taacaggaat attaattcct aaagcaaatt cggagttgat tgaaaattgt atagaagctt
     8161 tatattctat gaatcttcaa gatatgtatg ttattccaat aatagagaca agagaattta
     8221 tatataatga aaaaaaagaa cttagtttta ctaatttgta caatgctatt ttaagacata
     8281 agtcaagaat attaagcatt agagtaggca taactgatat attagggatg tatggtataa
     8341 ggagagataa aaacttctct atatataata atttgatttg ttcttcattt atattagata
     8401 taatcacata tcttcaaaga ccagagttag atatacctat aagtggtgga gtatcagaat
     8461 tttttgatat gacaaataag gatataagaa ataagtatat agaagaaata ttattggata
     8521 aatatcatgg attaattgga aagaccgtaa ttcatccaat gcaaattcaa attgtacaag
     8581 cgctatctac agtatcatat gaagatttta ctgatgcttt ggatatactg gatagtacag
     8641 acagtaagta tggtgtgagc aagggtgtat taggagagag aatgaatgaa acaaatccac
     8701 attttttatg ggctaaaaaa acattgattc tatctaaaat atatggtgta ttaaataaag
     8761 gagtagacta tgaagaattg cttaaatttt aattgtgaga ttgacattat tgataatcca
     8821 ttgaatttag aactaaatga ttttttagac gtaggagtaa gaaataatag caaaagacgt
     8881 tttttgttta tctcaaagaa actcggtaaa catttagcat gtaagccaag tgagatggat
     8941 aatttgggaa aattaatggc tacaatttat aatgaaaaaa ataaggaaga atcaaatggt
     9001 actgttatag cttttgctga aactggtaca gctgttgggc atagtttttt tgattactta
     9061 tgtggagatt atgaatttat tcatactact agagagcagt ttgacgagtt agaaaatctg
     9121 gaatttttag aggaacattc ccatgcaaca gaccagaatt tatactttgg tatgcttaac
     9181 aactttaaat ttggagatga gataatattg gttgatgatg agattaccac agccaataca
     9241 tgcatgaata taattaggaa aattcaaagc atttatccta aaaagaaata cactatatgc
     9301 tcaatactca attgggtaag tgatgaaaat ttaagtaaag ttactcgttt agaaagagag
     9361 ctaaattgta agatagattt tgtatactta tttaaaggaa actttaaatt ttatatagat
     9421 gaagatatta tttcaaatta caacgataat tatagtatta ctaaattaga gactatttca
     9481 agtgataaag taaatttagt agtaaatcat atttatgttg atttagaaga ttatattgga
     9541 aataagaaat atgtaaaata tacaggaaga tttggaatta atagaagaga acaatttaga
     9601 ttaaaagaaa tcgttaagag agaaagtaaa aaaataacta tggccagtaa agaatcttta
     9661 aaaaatgaaa aagaaaagat tctattttta ggtactgaag aatttatgta tatacctatg
     9721 ctgtttgcaa aagaaaaaga aaatcaatat gatatatact atcattcaac aacaagaagt
     9781 ccaattatta atattgataa gaagcattat cctattaaaa gtaaatttat gctaaagagt
     9841 ttttataata tagatattca aaattatatt tacaatatag ataaacataa tttttctaag
     9901 tgttatttat ttgtagagct gaataaaagt gaaaattctt atctagaatt tattgatata
     9961 attaaaaaaa caagtataaa agaattaact atagtatgtt gtagctaaag tgttttaaca
    10021 ggataaaata tatcacgatt ttaagatagg agttgagtca tatgagagat tttagcacgt
    10081 attctaaaga tgatgttatt tttttattaa aagatatatc aaatttaatt gaagaagaag
    10141 gaaataaaga aagagaatta aaaatacaaa atggtagaca ttattcagaa atgataccaa
    10201 tagagtatga agttagtgaa gattatctga atttatatca tgagaaacta agagaaaata
    10261 aagataagct tgcattttct ataggagtta tgtgtgaaaa aataattaaa aaaaatggaa
    10321 atgatgtagt actagtttca cttgctagag caggaacacc tattggtatt ttggcaaaaa
    10381 gatatataag aagcaagtat aatgtaagtt tacctcacta tacaatctct ataataagag
    10441 ataagggaat agatatgaat gcaattgaat atatagtaaa gaatcatccg agctcaaaaa
    10501 ttcaattttt agatggatgg actggtaagg gaactatatc aaaagaactt gaaaaagcat
    10561 gtgatgaact taatgttaaa tttaaaaaac attttgactc aacacttgct gtattagctg
    10621 accctgctgg atattcagga ctttatggaa ttagagaaga tttcttaata ccaagtgcat
    10681 gtttaaattc aaccgtttct ggtttagtaa gtagaactgt actaagagag gatttaattg
    10741 gtaaaaatga ctttcatgga gctaagtttt atagacattt aaaagataaa gatgaatcta
    10801 tgaactatat cgaaactata gaagaatgtt ttaaaaatca atttaagaat atatctgatg
    10861 aagttgaaaa ctgggaaagt gacattataa caagagatgg atactttgat gttttaaata
    10921 taaaagaaaa gtataacata acagatatta actttatcaa accaggtgta ggggaaacga
    10981 caagggtttt acttagaaga gttccgtata agattcttgt taaagactta aatgataaat
    11041 ctttagacca tatattcata ttagctaagg agaaaaatgt tgaagttgaa caaatggatt
    11101 taaaagccta taaatgttgt ggaataataa aaaatatgaa agatatttag gaaatatatt
    11161 agacaggaga atattgaatg attgtttttt cagatttaga caggagtatc atttactcca
    11221 ataaattttt aaatgctgat agtaaatatg ctaatataga aatttatagg gaaaaagaaa
    11281 tatcttatat atctttagat actataaatt taataaagca aatacaatat tatggtatgt
    11341 ttatacctac aactactaga acagtggagc aatttaagcg tatagaattt aataaatatg
    11401 ggatttattt tccttggagt ataacttcaa atggaggagt tatacttaaa gataatgaaa
    11461 tattgaaatc atggagtgaa aaaatagata aactaaaacc tgattatgag cccattgaaa
    11521 gtatgattca taaatttaaa gactatttga atgtagatgg aataactaat tttaaagttg
    11581 cagaagatac atttttttat atagttgttg atttctcaag atttaatttg gattcaatca
    11641 aagaatatac aaatatattg gaatcaaaaa attggaagtt ttatgtgagt ggcagaaaaa
    11701 tatattttat accaaaagaa atttcaaaag aaaatgctat aaaatacttg actaaagaat
    11761 taggaataga atatttttat gcagttggag attctattat ggactatggt atgcttaata
    11821 tctcaaataa atcctatgta ttgaagcatg gtgatataaa taaaaatgaa atagaaaatt
    11881 catttatatc aagttctttc aaaggaatga gtggaactga agagatatta tctaacattt
    11941 tagacgagaa ctgcctactt aatatataat gactgatata taaataataa actagaaaaa
    12001 tctgaaataa attatagtaa agtttatcat actaatttat ttcagatttt tatcaattta
    12061 tatttaagta agaactcaat atgttaaaat gaaggtggag tcattgccca gactgagata
    12121 catacctcat catcataatt ttcatacaca tgtggtatag tactgttaaa ataaaaacta
    12181 tctccttcct ccaatagata ttccttattt ccaagtgtta tcttcatttt accttttatt
    12241 atatagccac attcttctcc ttcgtgtgtc acaaattcac tgtttttttg tgtatgacca
    12301 tctagcgtaa caagtataaa ttctattttt ttcccagata aattaggaac aagaagttca
    12361 taaaattttg tagaatcatt agttactatt ttttttcgat tatttttctt tactactata
    12421 tcagagtcct ctcttccaat ctcttcaaaa aaataaccta tatttatatc aagagctttt
    12481 gatattttcc acattgctac aatagatgga acaaccatat tacgctcaat ttgacttatt
    12541 aagcctgtac ttaaatctga attttttgct aaagtagaga tacttatatt tttagatttt
    12601 ctaagagatc taattttgct tccgacatct atattcatta gatacaaacc ccctcagaca
    12661 tgattataac atataattta taaagattaa taaaataaaa aaaagtttta taatgcatgt
    12721 attttttatg ttatattgaa ttttctgaaa agatgcatta taatagtatt aatattcaat
    12781 atattgaata ttgataatga tattttaatt taatgaaatg gaggtaatta agatgaagag
    12841 ttttgtaagt cctgataata tttgggtatt atgggcagtt gtaactggat gggcagcatt
    12901 tagtatttac ttggaacaaa agtacaattg ggcatcaaaa gtgtcgggag caataatagc
    12961 tcttgtagga gcaatgttat tatcaaatct aaatattata ccagtagagt ctgttgtata
    13021 tgaccaagta tggggatatg ttgttccttt agccatagct ttattgttat atcaatgtaa
    13081 tattaaaaag atatggaaag aaagtggaag attgctcatt atatttcttg taggttctgt
    13141 aggaactgta cttggagcta tgattggatt tttagcatta aaaaatgtag taccagattt
    13201 aaatattgtt gcagctatga tgacaggttc ttatataggt ggaaatgtaa actttgctgc
    13261 tatgtcaggg gcgtttgatg cgccaggaga attagtatca gcaacagttg tagcagataa
    13321 tcttttaatg gcattatatt tctttgtctt aatagctata ccatcaattg gattttttag
    13381 aaaacatttt aaacatccac atgtagatga aatggaaagt ataggtataa ctgaaggaga
    13441 aacagtagca gcaaattatt ggggaagaaa agaaatatct ttaaaagata tagcaatagc
    13501 cattggtagc gcctttataa ttgtcgcagt atctgtagaa ctatcaactt ggtttaaagc
    13561 tataatacca ttatcaaatc cttttttagc aatgttaaat actttatttg gaaatcaata
    13621 tctaataata actactttga ctatgttgtt tgccacattt atgcctaatt tctttggtga
    13681 tataaaagga gcacaggaat tgggtacatt tttaatatat atattctttg ttgtagtagg
    13741 tgttcctgct tcaataacat taataattca aaaatcacca ttactacttt tatactgcgg
    13801 aataatggta ctaataaaca tgctagttac atttatagtt gcaaaaatat ttaagtttag
    13861 tttagaagaa gctattcttg catctaatgc aaatattggt ggtcctacaa cagcagcagc
    13921 tatggcaata tcaaaaggat ggtctaagct agtagggcca atacttatag ttggaacatt
    13981 tggatacata gttggtaatt attttggtct tttagtagga aatattttga tataatttta
    14041 cgtgaaaaga agttgttttt tagaaagatt tttatttaat tggaggttgt tatgattttc
    14101 gatggacatt cagatacttg ggcagatgta acaataagaa ggcttaacgg ggaaaaagat
    14161 gtatttagaa gaatccatct tgataatttt aaaagagcaa atatagaagg agctattttt
    14221 gtaatatgga tagacccacc atatgataaa gagcctgaaa aaagagttaa acaaattata
    14281 agctctataa aagatgaaat tgaagatatg aaagatgttg taaacatagt aaaaaaatat
    14341 tcagatatag ataaaggtat aagtgaaaac aaaataaata ttttaattgg tcttgaagga
    14401 ttaagccata ttggaagaaa tatagattta ctagattatt tttataaaga agttggtgca
    14461 agacatgcat cattaacttg gaatgaacag aatgaacttg ctacaggatg tttaggggat
    14521 gaaaatcgtg gtttaactga aataggtaaa aaagcagtca ataaaatgga aaaattgggc
    14581 atgatagtag atgtgtcaca tttaaatgaa aaatcatttt gggatttaat gtctgttgca
    14641 agtaagccag ttatagcttc tcattctaat gctaggagtt tatgtgatgc taaaagaaat
    14701 cttacagata atcaattaaa agaaatttca aatacaggtg gattagttgg aataaatagt
    14761 tttagaggat ttactaatag taatcctaag aagcaagatt taatacattt ggttgaccat
    14821 atagactata tggtaaatct tataggtata gaacatgtat cttttggttt tgattttagt
    14881 gattacttag ggaaagagac tataggttct tttactggaa atgctagtgt gccaagtgta
    14941 gatggtcttg aaagcacaca tacagcacag aacatagtag tagagcttga aaatagaggt
    15001 tataaaaaga aagatataga gttaatctct agtggtaatt tttataggct ctttaaagat
    15061 atactaaaat aatataataa agaacttaaa tttataaaat agtatattta tacaataata
    15121 tctacatatt taaattggcg ttcataattt attaaattaa attttaatgt tactagaagt
    15181 ttcaattcta gtaacatttt ttatgttaaa aatagatata gatatatatt gacaatgata
    15241 attattattg ataaactata aaagtgaaaa tgataataat tttcatttta attaataaaa
    15301 gatttttaag gagggagaat gaagaaaagg tatttagtca taggaataat aattttgtct
    15361 tatttttctt tatttatagg agctgaagat ataaatataa tgcatatatt tgcaaaaaat
    15421 cagcataagc ttatgatatt tatcatgagt agggtaccaa gattaataag tatattgatt
    15481 gctggtgtag gaatgagcat agcaggtctt ataatgcaac agataagtaa gaataaattt
    15541 gtatcaccta caacaggtgc tacaatagat gcagctcaat ttgggatagt aatatgtatg
    15601 cttttagtac caactgcctc tatttttaca aagacaataa tagcatttgt attttctctt
    15661 gtagggactt ttacgtttat gaaaataata gggaagttac aatttaaaaa tataatcttt
    15721 gtaccattag taggtataat gtttggaaat ataataggtt ctatgacgga ttttatcgcg
    15781 tataaatacg atttgagtca aaatgttagt tcttggatgc aaggtgactt ttctatgata
    15841 cttaaaggaa attatgagat attatatatc acaatacccc ttataatatt agcatatatt
    15901 tatgcaaata aatttactgt tgttggtatg ggtatggatt ttgcgacaaa cttaggtctt
    15961 agttacaaaa gaattgtcaa tataggtctg ataatagtag cacttgttac agtatgtgta
    16021 gtcgttacag ctggtaatat cccttttata ggtctaatag ttcccaatat cgtatccttg
    16081 tatatgggtg ataatataag agcaagcata tggtatactg gtttacttgg agcggtattt
    16141 gtacttatat gtgatatttt tggaagaatt attatttatc catatgaaat atctatagga
    16201 cttacagttg gagttatagg aagtatacta ttcctttatt taatattaag gaggaatgtg
    16261 aatgaagctt agtctcaatc aaaaaattta ctttgtaagt atattactat tcatatcaat
    16321 agcacttttt ataatttatg gaatagacat gaatcattta gaatatgcat tatcacaaag
    16381 aataccaaaa atatttgcta tggtattagg tggaggatgt atagcattta ctactgtagt
    16441 gtttcaaaca attacaaaca atcaaatatt aacaccaagt gtactaggtt tagattcatt
    16501 gtatgtaatg attcaaacta taatagtatt catatttggt tcaagcagta gtctgattat
    16561 aaatgaaaat tataatttta ttattaatgt agctatgatg attggagctt cattgttatt
    16621 atataagatg ttatttgaaa aaaataaaaa taatattttc tttttaatcc tagtaggtat
    16681 gatatttgga accttattta aaagtgcaac aacatttatt caagtaatga tagacccgaa
    16741 tgaattttta gctttacaaa ctagtataat ggcaagttta aacaatataa acactaatgt
    16801 attgttaata gcatttataa taattattgc cataatacct tttatatatg atgaaataaa
    16861 gtatttagat gtattgtcgt taggtaaaga acaagcaatt aatttaggtg tagattttga
    16921 taaggcagtt aaaaaaatga taatattaat agcaatttta gtctcaatat caacagcact
    16981 tattggacct atgacatttt taggactttt acttgctaat ataacaagag aaatttttaa
    17041 aacatataag catacatact tgatttcggg ttctatgttg atagggatga ttacattgat
    17101 aataggtcaa ttttttatac aacatgtatt taagtttgat acgactttaa gtgtagtaat
    17161 aaattttata ggaggaattt actttattca attactatta aagggggcaa atagatgata
    17221 gagataaaaa atatatttaa aagatataag aataaaaatg ttgtagatga tgtctctttt
    17281 agtatagaaa agggaaagat aacatctttt ataggaccaa atggagctgg aaaaagtaca
    17341 gtactatcaa tagttacaag gcttatagga ggtgatggcg gagaagtaat aatagaagga
    17401 aaaagcttga ctaattatag caataaagaa ttagcaaaaa aaatagctat tcttaaacaa
    17461 tctaacaata taactttaaa gcttacaata agagaattag ttggatttgg aagatttcca
    17521 tatagtgaag gtaatctaac taaagaggat gaaaattata ttgatgaagc aatagaatat
    17581 atgaagttga ctgatataca acataagtat ttagatgaat tgagtggagg tcaaagacaa
    17641 agagcttata tagcaatggt aatagctcaa gatacagagt acatattact tgatgaacca
    17701 ctgaacaatt tggatatgaa ccattccgta cagatgatga aggtacttag aagtctttgt
    17761 gatgagttag ataagactat agtattagta atgcatgata taaattttgc atcatgttat
    17821 tcagataata tagttgcatt aaaaaatgga aaagttgaaa aagtaggtcg tacagatgag
    17881 atagtaaatg aaaaagtttt agaagatatt tatgagatga attttaacat taagaatata
    17941 aatggaaatc gaatctgtat ttatttttaa aatattgtat atgaaaataa gcttgctata
    18001 tgatttttta taaagttttt aaattaaaat aaagattaat aggagagagg aaaatattga
    18061 tatgaataaa aaagcagcaa tagtagcagc agtagctatt ataggattag tgacagtgtt
    18121 tgcattaggt ggaagtaaaa agaatgaatc aaaaacatca gaaaattcaa ataataccat
    18181 aaagataact cataacttgg gagaaacaga tgtaaaatta aatcctaaaa aagtagtagt
    18241 atttgattat tcggctttag atacaatgga tgcattaggt gtagctgaga atttagtagg
    18301 acttccaaaa acgagtttac cagcatcttt agaaaaatat aaggataaga agtatgcaga
    18361 tttaggaggt ttaaaagaac cagatttaga aggtataaag tcggcaaatc cagatttaat
    18421 tataataaat ggaagacaag aagattttta tgaacaattg tcaaaaatag caccaacaat
    18481 aagtacaagt aaagatgata agaagtactt agaatctgtt aaaaataata tagacaaaat
    18541 agcaaaaata tttggagtag aagaaaaagc aaatcaagaa tttagtaaaa ttgaaaagaa
    18601 aatagaaact ttgaataaaa aagtaacaga taaaaattta aatgctttaa ctataatggt
    18661 aaatgaaggt aatttaagtg tatttggtga agaatcaaga tttagtatat tatacaatag
    18721 ttttggattt gaaaataaag ataaaaatat aaaagaatca agtcatggac aaaatattac
    18781 ttttgaatat atagctaaac aaaatccaga agttatgttc gttatagata gaggaatagc
    18841 tactggtagt gatgtaaaag aaagctcaac tgcaaaatct gttttgaaca atgatataat
    18901 aaaatctatg gatgcatata agaatgataa tataatatat ttagattcac caacatggta
    18961 tgtaaatgat ggtggtctaa catctttaaa taaaatgata gatgatgctt caaaagcggt
    19021 aaattaataa tccgatataa tcaaaatgta ttttttgtaa aatgattatc aaataaacta
    19081 ggagttttta agaactccta gtttattttt tgcataataa agatatagtt ta
//
LOCUS       DAMKPG010000074        18927 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMKPG010000074 DAMKPG010000000
VERSION     DAMKPG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.078"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(66..2090)
                     /locus_tag="PC621_002677"
     CDS             complement(66..2090)
                     /locus_tag="PC621_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109824.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            2480..3148
                     /locus_tag="PC621_002678"
     CDS             2480..3148
                     /locus_tag="PC621_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109825.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            3150..3635
                     /locus_tag="PC621_002679"
     CDS             3150..3635
                     /locus_tag="PC621_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF4109826.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            3696..4817
                     /locus_tag="PC621_002680"
     CDS             3696..4817
                     /locus_tag="PC621_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF4109827.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            5098..6288
                     /locus_tag="PC621_002681"
     CDS             5098..6288
                     /locus_tag="PC621_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4109828.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    6422..6677
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6736..7737
                     /locus_tag="PC621_002682"
     CDS             6736..7737
                     /locus_tag="PC621_002682"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF4109829.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            7841..8230
                     /locus_tag="PC621_002683"
     CDS             7841..8230
                     /locus_tag="PC621_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF4109830.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            8391..9599
                     /locus_tag="PC621_002684"
     CDS             8391..9599
                     /locus_tag="PC621_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF4109831.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(9686..10762)
                     /locus_tag="PC621_002685"
     CDS             complement(9686..10762)
                     /locus_tag="PC621_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF4109832.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            10991..11518
                     /gene="hpt"
                     /locus_tag="PC621_002686"
     CDS             10991..11518
                     /gene="hpt"
                     /locus_tag="PC621_002686"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF4109833.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            11607..12539
                     /locus_tag="PC621_002687"
     CDS             11607..12539
                     /locus_tag="PC621_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF4109834.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            12601..13827
                     /locus_tag="PC621_002688"
     CDS             12601..13827
                     /locus_tag="PC621_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF4109835.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            13911..14132
                     /locus_tag="PC621_002689"
     CDS             13911..14132
                     /locus_tag="PC621_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF4109836.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            14321..14491
                     /locus_tag="PC621_002690"
     CDS             14321..14491
                     /locus_tag="PC621_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109837.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(14584..15045)
                     /locus_tag="PC621_002691"
     CDS             complement(14584..15045)
                     /locus_tag="PC621_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109838.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            15228..15695
                     /locus_tag="PC621_002692"
     CDS             15228..15695
                     /locus_tag="PC621_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109839.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            15738..17501
                     /locus_tag="PC621_002693"
     CDS             15738..17501
                     /locus_tag="PC621_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF4109840.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            17509..18117
                     /locus_tag="PC621_002694"
     CDS             17509..18117
                     /locus_tag="PC621_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4109841.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            18189..18758
                     /locus_tag="PC621_002695"
     CDS             18189..18758
                     /locus_tag="PC621_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF4109842.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         7256 a         1992 c         3202 g         6477 t
ORIGIN      
        1 atagaaaaat aaaaaattat aattatatat aaattttgat aaagtaaaga tgaattacta
       61 taggtttaaa ttatattatt ttctattatt tttatgcctt tctcagttat tgatgagcct
      121 tttcttcctt tactagaaat taagtatcca ttgcgtgtca aatattctag catatttctt
      181 acttcgtatt ctgtgacatt atattccata aatttcatta actttgtcct ccccattggt
      241 ttcatgtgca atgattttag gatagaaatg catatgtttt tgtcatattg gtttaaatca
      301 ttgaatatag acatattact tgacatatga ttattattta attttgaaga tatgttttga
      361 ggtaaatcat ttattgtaag ataattggaa cccatgtaag ccatatattc aacacaattt
      421 ctcagttctc taatattccc catccaagtg tatgaattta aagcttctaa aagactctta
      481 tcgagtttta tgtttttata gggaagttct tttaaaaatg attctaaaag aagaggtata
      541 tcttctaatc tctttctaag aggaggtaaa tctaaagtga acatacttat cctataaaat
      601 aaatccatcc taaatttatt ttctataatt aatttttcta aatctttatt tgtggcagct
      661 aatattctca catcaattgg aataatactt gtaccaccaa tttttctaat ttctttttct
      721 tgtaagactc ttaaaagttt aacctgtaaa tcatgtggca tatctcctat ctcatctaaa
      781 aatatagtgc cagtatgagc taattcgaat aatccttttt ttccaccttt ttttgctcca
      841 gtaaaagaac catcttcata tccaaatagt tcactttcaa gtaattcact tggtaatgaa
      901 gcacaattta ttgctacaaa aggtttattt tttctatttg aatgattgtg tattgactga
      961 gtaaaggctt ctttacctgt tcctgtttca cctgttatta atataggatt atctgtaaga
     1021 gccatttttt ttgccctttt aatacaatct tttataatag aactatttcc aataatatta
     1081 ttaaatttat atttagctac ataacctctt ttgacagaat cacttcttat tttttgttct
     1141 aatatttcta tattgtttac atcttttaat gtaattaagc aaccctctat atttttataa
     1201 agataaaatg gttttttaga aagtatgaat tttttatcta tttcgtctat gtatataatc
     1261 atattatcga tatttaagtt ctttgtaatt ttatctaaga aactctttgg catatattcc
     1321 attaaggaag gtgattttat aagtttttca tctagttcca acatattaag taaagactta
     1381 tttacaaaag taaccttgtt aaaggtgttg aaaaatatta gaccatcacc tatttcatca
     1441 atggtcatgt aaagtatagt ttttaattta gatatattat ctaatgacgt atttaagaaa
     1501 tcatgactta gtgtctcctt ggacaatttg tatagttttt ctataaattt ctcatttttt
     1561 agttttaata ctactaataa tgacatatat gtatctaatg agatttttct ccaacctaaa
     1621 tcaacaatat tttttatgta ttttggtata aatctaatct gacctggtgt tatagctgta
     1681 tctaaatcat atatttcatc acaatctgga tatgtcggta caaaatctat atgatttata
     1741 ccaattttat atagataagt agccatctca aatgctgaag ttttattggt tgtcataagt
     1801 aacgattttg tacctggagg tagtttcatc attttttcat atccaagttt tgaaagtgct
     1861 ttcgttccat gaataatttc aatatcttcc ttaaaatgtt ttattaaaaa attactagtt
     1921 aaaggtgata gagaaagcac tacctctccc tctaaatttt cttttattcc atcaagtata
     1981 ttttgtgttt ctatgattat ttcgtctcca aaaaagtttg ttaattcttc tttgtagtgg
     2041 tcactattta atttatcata tgttataagg attaacttct ttttatccat tttatttctc
     2101 ctaacaaaat agtatttatt tttaaagtat ttaaagtata aggtaagtta tattatacaa
     2161 caaatagtag tataaattta tatatttgat taaataatag gtttttttag gattttatcc
     2221 tataaaaacc tattttaaaa ttatttttgc aagataagga aaatagcttt ttatattgta
     2281 atgtatattt taaatagtta ataaaaaaat attatatttt taaacttgaa aagtaagtaa
     2341 ttataagcta tttaatcgct ggaataaatt tacaataaaa aaataaatac aaaggcttta
     2401 tcattaaggt attggcataa gttttgctta ttaaacaagt atactaataa aattcattta
     2461 aacaggaggg agaatttata tgagtaaatt agaatcaaca acagaaaaag ttgaggtaaa
     2521 atggtatggg tatgtagcat taatattggg agcattattt ttttcaggaa tatttaaaga
     2581 tgcaccagga ccattaaaag tattggattt taacaatgta ttaggttctt ttggaacatt
     2641 gggaacaatt aatgatggtg taggtacatt agcttcaaac tttagaggtg atggaggtac
     2701 tggccctcga gatggatggc tatatgcatt gacattaata ccatcagtta tgtttgcact
     2761 tggagtagtt agagttatag accacttaga tggaatgaag gcagctcaaa agcttcttag
     2821 tccactatta aagccattat tagggttacc tggatttgca ggactaactt tgatagctag
     2881 tttacagtct acagatgcag ctgcatctat gactaaagaa cttaaagatg atgggtatat
     2941 agatgaaaaa caaaaagctg tattttgtgc atttcaattt tcaggtgcgt ctgcaattac
     3001 gaatttcttt gcttctggag cagcactatt tccatttata ggagatgtac caatatttat
     3061 accacttgct ttaatattaa ttatgaagtt tgttggggcg aacttactta gattatattt
     3121 aaataaattt gacaaggggg aagcttaaca tgagtgaggt agcaaaaaaa gaagtaaaag
     3181 ttaaaaaagg aaatgtgata gatgcgttta ttggaggagc tagaaatgga ttccaaataa
     3241 gtacaaattc aatggctcca aatgtcatct tcggttttgc tataatttct gtgttcaatt
     3301 taacaggtct tttggatgta atagggacaa tatttacacc tgtaatgagt atctttggac
     3361 ttccaggtgt tgcagcaaca gctataatga caatatttat atcaatggga ggagcatctg
     3421 gagtaatcgc aggtttattt acagcaggtc aaataagtca agcacatgtt gcaattctat
     3481 tgccaatgtt attgctttca ggagcgcaga tacaatttat ggggagactt ttgggaacag
     3541 ctgagttaaa aactaaatat tatccacata tgtttgttat agcaacttta aatggttgta
     3601 ttgcaatgct tataatgaat tatttcgtag tataggtaat tataataaaa atttaatata
     3661 aatggaaagt ttaaatttta aggaggagct agattatggg aactaacaaa gattatattg
     3721 agtatgtaca taaagtgtat gattatctac acactatacc agaaaaagga tttgaagaat
     3781 ataagacatc agagtttgtt ttaaaagagc ttgaaaaaat ggggtttgaa aatattaata
     3841 aaggagttgc aggaactggt attatagtaa ctatagattc taaaaaagaa ggaccagtat
     3901 ttgctataag agcagatatg gatgcattgg aatttatcat agatggagaa aagaaaatga
     3961 tacatgcttg tggtcatgat ggtcatactt ctatgctttt aacagctgca aagttaataa
     4021 aagagagaaa tttagtaaaa agaggtaagt taaaaatact ttttcaacca ggtgaagaaa
     4081 aattatatgg agcattaaga gtaattgatt ctggtttgtt ggatgatgta gaagaaatgg
     4141 tagggatgca tttaagacct ggacaagatt gtaggttggg aaaggcgata cctgcaatgt
     4201 accattgtgc tagctgtata atgcaattta cgattaaagg tatcacttgt catggttcta
     4261 aaccacattt aggagtaaat gcagtagatg ctgtctgttt agcagtgaat gcaataaatg
     4321 caataagagt aaatccagct ataccaaatt ctataaaagt aacaaatata tcagttaagg
     4381 gagaaactta taataatata ccagatgaag catttatggc tttagatata agggcagaaa
     4441 gtaatgatat aatagaagaa ttaaaagaaa aagcaataca tgctgtaaaa actgctgtaa
     4501 gttctataaa tgcagaagct gtactaattt ccaaagatgg agtaccagca gcagaatttg
     4561 atgaagaact tgtaaacttg gctgaagaaa ctataaaaga tgtattggga gctgatgcat
     4621 ctataggtat atttaaaaat tctggtggag aagatttcca ttattataca caaaaactaa
     4681 aatgtaaaac tacatatatt ggattaggag ctgatgctac accaggattt cataatccaa
     4741 atgtaacatt tgatacgaaa gctttagaat atggtgtaga tatatggtgt agattggttg
     4801 aaaagagatt gggataatat ccaaactaat aattctacat ttaaaattat aattatagaa
     4861 gttatagaaa aagattgctt atgttaatta gaaaataatt aatatgagca atcttatttt
     4921 gatataatga aactgatttt ggaatattga aaaaatatga tggaaaagta attatatttt
     4981 aaatctattg gacaaaaaag tgatattatt ataatattaa gaattgtaaa ataataccaa
     5041 agtgcatcta gtaatggact taacaattat aaaaatttta ttcttaggag ggaaaaaatg
     5101 agtaagtata attttgacag agtaatagat agagtaggaa cagattgtgt aaaatgggat
     5161 tttagaacta attgttcaac aaaagcacaa aaagatggtt tgcctttttg gattgctgat
     5221 atggattttg aatgtgcaga accaataata gaagcattac gtaaaaaagt tgaacataaa
     5281 atatttggat atagttcaaa tgatagtgat aaatacttta atgcagtttg tgattggtat
     5341 aaaaggagat ttaactggga aataaataga aaagatatta tttttagtcc tggtgttgta
     5401 cctgctgttg ctattttagt aagaatatta actaattcta atgaaggtgt aatagtccaa
     5461 aaaccagtgt attacccatt tgaagctaag ataaagagca ataataggga cattgtaaac
     5521 aatcctctaa tatatgagaa tggtacttat aggatggatt atgatgattt agaagaaaaa
     5581 gctaagtgta gcaacaataa agtactgata ctttgtagtc ctcacaaccc tgttggaaga
     5641 gtttggagag aagatgaatt aaagaaggtt gtagaaatat gtaaaaaata tgacctatgg
     5701 ataatagctg atgagataca ttctgattta gttagaaaag gatttaaaca tactccttta
     5761 cagtcattat gcccagagta caaggataaa ataattacat gtatagctcc aagtaaaaca
     5821 tttaatctgg caggaatgca gttgtctaat ataatagtga ataatgatga actaaaaaag
     5881 aaatatcaag aagaggttac agctgttgga gttgcaactt caccaaatcc ctttgctatt
     5941 gtagctacaa tagcagcata taatgaaagt gaagactggc ttaatgaatt aaatgattac
     6001 ttagataata atatacaatt tattgatgaa tacttaaaag aaaacctacc aaaagtaaaa
     6061 cttgtttatc cagaaggaac atatctagca tggttagatt ttactgctta tggattaaat
     6121 gaagtagaac ttgaggattt aatgtttaaa aaagctaatg tattatttga tgaaggttat
     6181 atatttggaa aagaaggaat aggatttgaa agaataaatg tagcttgtcc acagtcttta
     6241 ttaaaagaat gtatggacag attaaagact atatttgaaa atttataaag tataggtaaa
     6301 aatttataaa gtgtttaaaa ataaaaaaat aataaacttg acaaatttta actaatcact
     6361 tatcatacta ttatgaataa atagaaaaca taagagagct aaattttatt gaattaaact
     6421 cttaaagact ttgaaaggaa gagtagttat agaatgtaat taaagcgagt tggtgatggt
     6481 gtgagaccaa tatgaagtct ataatgaaga acatcctgga gttgctaacc gaaattttaa
     6541 aagagtaggc ttagtcggaa ccaaaccgtt atcatattgg ggtgtatcga gtaatatctg
     6601 tacattgtga gctgggtttg aatttatttc aaactaatca gggtggtacc gcggaaaagc
     6661 aacctttcgt cccttttata tagggatgaa gggttttttt aatggaaaaa tatataaata
     6721 tagggagtga atgatatgag cacaataata ccaaaggatt ataaaagtag tttgaacgta
     6781 attgatacac aaatagctat aaaaaaatta aaagattttt ttgaaatgag actaagtaat
     6841 gaattaaatc ttataagagt ttcttctccg ttatttgtat tgccagaaac aggtgcaaat
     6901 gataatttaa atggagaaaa ggctgtaagc tttgatatac catttatgaa taaaaatgca
     6961 gaaattgttc aatcacttgc aaaatggaaa agattagccc ttaaaaaata tgggtttgaa
     7021 gttggtagtg gtttatatac agatatgaat gcaataagaa aaaatgaaga attagacaat
     7081 atacattctc tttatgtaga ccaatgggat tgggaattgg ttatagataa aagtagtaga
     7141 aatgaaaaga ctctaaaaga tgttgtaaaa agattgtatg gagtatttaa agatactgaa
     7201 atttttgtat gtagtatgta tgaaggtata aaagaaatat taccagaaga aataactttt
     7261 ataacatcac aagagttaga agatatgtat ccagaattat caccgaagga aagagaagat
     7321 aaaattgtaa aagagaagaa agcagtattt ataactcaaa taggaaaaac attaatatca
     7381 ggagaaaaac atgatggaag aagccctgat tatgatgatt gggaattgaa tggggatata
     7441 ttattttaca atcctgtttt agattcagca ttagaattat cttcaatggg aattagagtt
     7501 gatgaagaat ctcttgatag acaattgaaa cttgctggat gtgaagagag aaaagagttt
     7561 gactatcata agatgctttt aaatggcgaa cttccgtata caattggtgg aggaattggt
     7621 cagtcaagaa tatgtatgta tttcttacaa aaagctcata taggagaagt acaagttgga
     7681 gtatggccac aggatatgat tcaaaattgt agtagtgcag gaattgaatt attataattt
     7741 aaatataaac taattgataa aatttatatc taatatgttc atcaaattaa ttatatcggt
     7801 tataatgtag aataacctat aattataaga ggtgataaat atggagtttt taaaaataat
     7861 cattaatata gttttagata ttttaaaaaa aattttggtc agatttaaaa atgctaaatt
     7921 tggtttggtt tttgtatttg atttacttaa actacctgat tttatgacag ataaaagaat
     7981 caatatagtt gataaaataa aggtaatttc tgtattaatt tttactatat cctactttgt
     8041 atctggggtt gatataattc cagagatgat tgcaggggca tttggtttta tagatgatgc
     8101 tatagtttta atttggagta taggtatagt gaatgaagaa attaataagt atagagttat
     8161 tataaaaaaa gataaacatt caaatataat tgaaaatgta gaattttcta taaaagatga
     8221 agaagaataa caatttatat atgttattct tttttttatt aacaaaaaat ggttaaattg
     8281 ttgacggaga ggtaaaactc tattatcata ataagtggat atccaatatt taactaagta
     8341 ttcaatgtgc aagaatgctt aatttaaaaa agttatggag ggtaaatatt atgaagaaaa
     8401 aaataggttt agtgcccaaa ctaatcatcg gtatcatagt cggtatttta atcggaagtt
     8461 atgcaccaga aataatagta caaatattag taacagttag taccttattc tcagcattcc
     8521 taaaatttgt tataccattt ataattatag gttttgtaac agcaggtata gctgatttag
     8581 caacaggtgc aggaaagtta ttggggataa ctacgggaat agcatatggt tctaccttaa
     8641 tagcaggatt attatcattt gcagtatcta cattaatctt cccaaatttt attgatgcaa
     8701 gtgttgcaag tcaaattgga gacccagagg caggaatgtt agctccaata ttcacaattc
     8761 cattagagcc tatggtagat gtaacagctg caatcgtatt tgcatttgta atgggacttg
     8821 gaatatcagc tttaagaaat catggaaaag gagagacttt atttaatttc ttccaagaat
     8881 ttcaaggaat cgtaacgaaa acgttgtcaa cagtgattat acctttatta cctttataca
     8941 tagcaggaac ttttgcaaat ataactatag caggggaagt atggacaata ttaggggtat
     9001 tctggaaagt atacttagta gtaatacctt tacattttgt atatatggca tgtcaattca
     9061 cagcagcagg tgtattttca ggaaaaaatc cagtaagaat gttaaaaaat caagtacctg
     9121 gatatttaac agctatagga actcaatctt cagcagcaac tataccagtt aatgttggtt
     9181 gtgcagaaaa aaatggagta agtaagcaaa taagagaatt tgttgtacct ctttgtgcaa
     9241 caatacattt atcaggtagt atgatatcaa taacaggatt tacaactgca gttttaatga
     9301 tgaatggtat gccacatgga ataggtgtat tattccctta tattgcaatg ttaggtatag
     9361 caatggttgc agcaccagga gcaccaggtg gggctataat gagtgcactt cctttcctac
     9421 caatggttgg aataccatca gatggtgggc ttgcaagttt aatgatagct ttatacttaa
     9481 ctcaagacag ttttggtaca gcatgtaatg tatcaggaga taatgcaata gcagtagtag
     9541 tagataaaat aaatgatgga atgagtaaaa aaggaaacaa agaagagcaa agtgcttagt
     9601 tttttaataa tcaaaaatga gttatctcaa atagagatag ctcatttttt atggtataaa
     9661 aaaattataa aataaaggga tttttttatt taaaaattgc tttaagagat gatattaata
     9721 atttattttc attaggtttt aatatacata ctctaaagaa atactcatct agaccttcaa
     9781 atgaacaaca atctcttatt attatttttt gtggaagcag ttgttctcga agcgatttag
     9841 cagttaattc ctttgttttg attttacaca atatgaaatt acctttagta ttgtatatat
     9901 ctaagctttt tatattctta agctctttaa gcaggtaatc tctttcagtg ttcattaagg
     9961 aaatagtatt tgatataaaa tctaaatcac taaacatgat ttcccccatt ttagaagcta
    10021 ttatgtttat attccataaa tctagatttt tatttatttc atttttgaca ttagtgttag
    10081 atataagacc atatccaagc cttattccag gcgttgaaaa aaattttgat gtacctctta
    10141 taacgaataa attagaataa tcatcaacta attgagtaca agagtatgta tctgtatcag
    10201 taaactctac ataagtctca tcaatcatta aaaatgagtc tgtatttttt aaaatttctc
    10261 tcacttctac tttagtaaat gcaaatccag ttggattact aggattacag attactacca
    10321 aatcataatc tttagcattg atagttttta taagattttc taaatttata tgaaaattat
    10381 cttcttcttt tgcaaagtat ttttctatag aacaattaat tttagataac tctttttcat
    10441 actctgaata ggctggagag agtaaaagag cctgtttagg attaattgta tgtataaatg
    10501 atgaaattag ttctgtagct ccactgccaa gtactatatt atctatagaa catttgcagt
    10561 aattggatat agaagttttt aagtctatgt aatcagggtc aggatacata gataccatat
    10621 ctatattgtt tacaatgtat tgtttggcta aacttgatgt accaaaagga tttatatttg
    10681 agctaaaatc cataaattct tcctttgaaa agccatactt actagataaa ctatataaat
    10741 tagctccatg atttgtgctc ataaaagtac ctccaaaaat aaaattatat aatcgataaa
    10801 aataactgat taatatatgt acatatagat tataatacaa aggaatcgtt attccaaatt
    10861 tttaatggaa aaagcaaaaa agtatggata agctgttaat ttaggtctaa aaatgatgaa
    10921 aaagatttta attgatattt tggtatgcta taatatataa gttaatagaa ggaaagcggg
    10981 aggatacaaa atgtacaaag ttacaggaaa aatgttgaca gaagaacaaa taaaagaaaa
    11041 agtttatgaa ttaggaaaaa agatagaaga agactttaaa ggcgaagatt tactagtggt
    11101 aggtatactt aaaggagcta gtgtatttgt ttcagattta ataagatgta tagatttgga
    11161 tgtaaatata gattttatga gtgttacaag ttatggaaac tcaacagagt cttctggaac
    11221 agttaaaata ttaaaagatt tagatgtaga tattgaagga aagaatgtgt tgattgtaga
    11281 ggatataata gattctggtt taacattaag taatctagtt gcagctttaa aaactagaaa
    11341 tccaaaatct ttaaaactat gtactctact tgacaaacct cagagaagaa aagctaatat
    11401 acctgtagac tatgttggat ttgttataga agacaaattt attgttggtt atggaataga
    11461 ctatgctgaa aagtatagaa atttacctta tataggtata gttgaggatg tagaatagat
    11521 ttaaatgtga atcaaaagca ctaatttcta agggagttag tgctttttag ttaattatat
    11581 aaaattttag taaaggagac atctatatgg atattcagtt aaatatatta ttatttttcc
    11641 aaaatttaag aaatccaatt ttgaacttta tatttcttgt ctttacaata agcacagaag
    11701 caccgttatt aatattaatt acaacaatta tatactggtg tataaataaa aaatgtggac
    11761 agaaaatact gtttgctata attggaaatt ttgttgtaaa tttaggcatc aaagagtttg
    11821 taaaagctcc cagaccaata ggaataaaag gtcttgaatc attgagagtt tctactgctg
    11881 gtggttattc ttttccaagt gctcatactc aaactgcaac aacattttgg gttagtatta
    11941 tgactatatt taaaaagaga tatcttcatt taataggtgc agttatggtt ttaggagtag
    12001 gattgtctag gctgtattta gctgttcatt ggccaataga tgtgatagtg ggttggatgt
    12061 taggaatatt ttttacagtt atatttataa aaatatttga ctatattgat gataataaaa
    12121 attatatttt acttgtagtt ttacttatac catttataat agtagctata tttctaaata
    12181 gccctgaata ttttgaaaaa tttggtataa tagtaggatt tgtatttgga tatatggttg
    12241 aggatagatt tgttaaattt aatacagata ataataacaa aaaaataaat tttagcaata
    12301 aaaataaggg gagtaaaaat ataatttttt caagaatatg taggtttttg gttgggatag
    12361 taacaatagg aatgttatat attggcatta agttgtttat ttcaatgtta atagtcactt
    12421 taaatataag tgactctact atgagtataa tttttatgaa ttttattaag aatacagtag
    12481 ttgtttttta tggaatagca ggagctccag cattatttaa acttttaaaa ttaaattgat
    12541 tttaaggtat agagtcaata gtttaaatta aaaaaattaa gggtagaaaa gagtgattat
    12601 ttgaagaagg tagtaataat aggtggtgga ccagcaggga tgatagccgc atcaactgca
    12661 tgtgagaaag gctatgatgt aacattaatt gagaaaaatc ataaattagg aaaaaaactt
    12721 gcaataacag gaaaaggtag atgtaatatt actaacgctt gtgaaataga ggaattaata
    12781 gaaaatgtac ctacaaatgg aaaatttctt tatagtgctt tttatacttt tacaaatgat
    12841 gatgtaataa gtatgtttaa taatcttgga gtaaagacta aaacagaaag aggaaaaagg
    12901 gtttttccag agagtgataa agcctttgat attgtaaatg ctcttgaaag acaacttaaa
    12961 agtaagaaag taaatatatt attaaattca aaagtagaaa aaataatatc taaaaataat
    13021 aaaattgaga aagtaatttt aaacgataaa aaagaaatta agtgtgattc agttgtagta
    13081 gcaactggtg gattaagtta tccacttact ggttctactg gtgatggata taaatttgca
    13141 atttcacagg ggcacacaat tatagataca aaaccatctt taattggtat agaagtacaa
    13201 gaaagtttta caaaagattt agaaaaatta tcattgagaa atgttgaaat aagggtgttt
    13261 aatagcaaac aaaagaaagt ttatagtgat tttggagaac ttgaatttac tagatttggt
    13321 ttagatggac ctataatcaa gtctgcaagt tgtagaatga aagatacaag gaaagaaaat
    13381 tacactatat tattagattt aaaacctgca ttagatgagg aaaaactgga taaaagagtt
    13441 caaaaagatt ttcagaagta tacaaataaa aaatttgaaa aagcattaga tgatttactt
    13501 ccaaaaaaac taatacctat aataataaac ttaagtgaaa ttaatgcaaa tacggttgtt
    13561 caccaaattt ctagagagca gagaaaaaac ttagtccatt tattaaaaaa tttaaagttt
    13621 acagtaaaga gatacagacc tattgaagaa gctataataa cttctggtgg tgtaaaagta
    13681 aacgaaataa attcaagcac aatggaatca aaattagtag aaggtttatt ttttgctgga
    13741 gaagtaatag atatagatgc atatacaggt ggattcaatt tgcaaatagc tttttctact
    13801 ggatatttgg caggttttaa ttgctaaaaa ttacctgaat taaaaatgtt taaaatcaca
    13861 taatattatc agcaacacaa aataaattaa acagaaggaa ggtaatcaac atggcaagta
    13921 acaataacaa caacagaaca gtagtaccag gagcaaaaga ggctttaaac caaatgaaat
    13981 tagaaatagc taatgaactt ggtatgagta attatcaaca agttgataaa ggaaacctta
    14041 ctgcaagaga aaatggttat gtaggtggat acatgactaa aaaattagtt gaaatggctg
    14101 aacaacaaat ggctggaaaa agcaacagat agttataaat aattatttat aactattggc
    14161 tattagttta gctagagcta tctcaaatat agaaagtaat ttctaaattg agatagcttt
    14221 tttttattaa attattaaat agatatattt ttatgtaata aattttatgt atttggataa
    14281 aataatacaa atgaatatgt tacataagga gtgatgtaaa atgagtagtg taaattttga
    14341 ggatttaaaa aacaaattta ttaatagtga cttagatgaa aaaataagaa tatatacaac
    14401 aacagaaggt ttatcagtag aacaatttaa agagctacta aaatattatc caatacaaca
    14461 tttatctaaa ctagaaaaag cacttggata gcagtattta gtccaaaatt aaaaaggcaa
    14521 tttatttaat attaaaaata ttttttatta aataaattgc ctttttataa taggtttaaa
    14581 ttattatgct tttgatatat aattatagat tctctcattt ccaagttcaa tagacccagt
    14641 attaataaga gtaacgattc cttctatatc tctagcagtt aagtatttat atggaattaa
    14701 taaattataa ccagtattac ttagtaagtt ttttaattgg gttatttgag agatattgaa
    14761 acattttgca gttttagtag atttttttct taagttagat aaattttcat tttgaatatt
    14821 tattaaagtt tctatatagt ttctataaga tatcttaaaa tttgtctcat tgactatttt
    14881 ttcattaagt atactactga taaaatgttg agacaatata ttatctgcaa atttatctgc
    14941 tgttaatttt ccagatgtta tgcctttttg atattttcta agaatttttt tagtatagca
    15001 gtatatttta tttctcgttt ctaaatcgag ttgttctatt tgcacaaaac cacttccttc
    15061 taattataaa aatatttatt aaaaatctat acttaactaa taaagcttca ataatttatt
    15121 tacagtaaat actaaatcat aaaatatttg ttatttttta ttattaccaa gtataaaaaa
    15181 tattataatt gtaaaactta caaatatatg taaaatattt aggagtgatg aaaatgttaa
    15241 atgggataat aaagcttttt tattctgaaa ttgtaaaaac aaagattaat aacctagact
    15301 caagtggata ttctttaaga gatgaatgta aagaaatcag aaatataact ctaaagatag
    15361 atagcgtatt ttttaagctt ttaaagttgg cttttggttc aatactgctt atgatagctt
    15421 ttaatttaaa catgattcta ggtataggag tgtttttaat tggtgtagtt tatatactat
    15481 ataaaagaaa tatggaagaa caagtaaaag agcatataaa taatgtgaaa aataatatcg
    15541 acatctctaa aataactttt atggatgaaa aagggagaac aggtctaaat gcattgatta
    15601 cattacttct tataggatta ttgacaggat ttaactggat attagttgct agttttgtcg
    15661 ttgtttttgt ttttactata aagaatgtat gttaaaatac taataaatag tttaatatat
    15721 agggggagaa attgagtatg actaatggca gttataaaat gaagaattta gtggagatat
    15781 ccgctatggt gacggaatct acagattttt ttaatataaa agatgacata atagaaaaaa
    15841 tgttagaagt tgttcaccct acaaaggcat gtgtaaatct attttataaa aatgattcta
    15901 aatatgccta cttagtatgt tcgcaaacct tagaatatgt acctcaaatt tttccaataa
    15961 atagtcttaa aggtgcaaaa attgattttg acacatatcc agagtatata catgaggctg
    16021 taaatgaaaa aaagataata tatgtggaaa atgtatttga agattctaga gcagaaggtg
    16081 aacgagattt agcaaaagct gaaggttata ttgggagaat agtttttcct tttataataa
    16141 ataatgtagt tgttggattt atgacttgtt ttttaaaaga ggatgattat ataacagaac
    16201 aagatataga ttttatatca tctgttgcat cactcttaag tttatcaata gaaataacta
    16261 ataaaaataa taatgtacaa attttaattg ataaactaag aggctcaata agtgccataa
    16321 atgaagctac taaaaaacta taccttaata agagtattga tggctttttg gaaaatttaa
    16381 gtaaacaagc gtgtaatatt actaatagta aagaagcttt aataattata aatagtcatc
    16441 ataataagga tactatagtt agttgttata atgttgagca aaaaaagaaa acaaatttat
    16501 atcctatgat tgatatgttt ttaaagaaag agtcattagg gggataccta aataatttaa
    16561 aaccatgctc aaaaaagggc ataaatttgg aaagttatat atattacaaa cttcaggata
    16621 aaaatgagac tataggatgt atagtttgtg caaatagtaa aaactatact agtgatgatt
    16681 taaatattct tagtatattg tccaaacaag tttctgttgg gatgcaatta tatgaacata
    16741 atgaaaagga agtaaaacac aaagttcttg aaaatgagtt gaacatatta aataaacaac
    16801 aacagcttat tatggataaa ggtaaaatgg attgtaatga tgagaaagaa ctttattttt
    16861 accataaacc agcaagggta gtaggtggag atttttatta tgctacaaaa gtggatgacg
    16921 aacatatagt ttatattgtt gctgatgtaa tgggacatgg aatggtatca aattatgttg
    16981 ttgcaatgat aaaaggtgct tttaaagtat tatgcaatca atacaataca ccaagagaaa
    17041 taatgacaaa tttaaatagg atgttatatg atgaatttga caagatggga gtatttacaa
    17101 cttgtttgat aggtattata gatactaaag aaaatatgat tactgtgtct aatgcaggtc
    17161 actatagtcc tgtagttgta aagaaagatg gcacaataga aaataattta aattgtaaaa
    17221 agggcatacc tattggaata atgaaagatg ctacttatga aaataatact ctttcaatgg
    17281 aagattatgc aatggtatgt atgtacactg atggaatttt agagataaaa aattcatcaa
    17341 aagaagagta tggtataaac agattagaaa actttctaaa agaaaatttt agattaaatc
    17401 aacagttaat agtagaaaat ctaaaattgg atttaaagaa tttctcatca aaagataatt
    17461 atgatgatga tattttaata gttatgttaa aggataggta gattgattat ggattcaaac
    17521 gataaaaaag gacttaaaag tacagagatt ataataataa atggaagtcc aagaattaat
    17581 aaaaattgca gttttatagc aaataaagta aaagctatgc tagaagagtg tgggttaagt
    17641 tgtaaaatat ttaatatcta caatatggat attgaatatt gcacagcatg tggattttgt
    17701 gaaaaaacag ggtattgcaa gtttaatgat gatatgaagc cattatataa aatgtttgat
    17761 gattcaaaag gaactatagt aataagtcct gtacattttg attcaatatc agctaaatta
    17821 aagactgttg ttgatagaac tcaagctata tatgcaagta aatatgtatt aaataaacct
    17881 tcaattgata gaaataagaa acggatagga atgtatattg cagtgggggg gagtaaatca
    17941 tatgattcac agtttaaagg tggacagatt gttatggatt ttttctttaa gagtataaat
    18001 actaaacttt tacacaatat atatttaaac aatacagatg atgtatctta tatagataat
    18061 caagaattta aagatataat atataaaaat ttaaagatat atattgattc aatataattt
    18121 gattttgtag gtaatattat gtataattgt attgcaataa tttaaataat taaaaggagg
    18181 gaatttcaat gaaattacca atagcattat ctaataaaca tgttcatttg agtcaagctg
    18241 atatagacgt attatttggg gcgggtcatg agttaactaa atttaaacca ttatctcaac
    18301 cagggcaata tgcctgtgaa gaaaaagtgg atttagtagg accaaaagga actttaaagg
    18361 gagttagagt actaggacca gctagagctc atactcaagt tgaaatatct ttagcagatg
    18421 gtttcccatt aggagtaaaa gcaccaataa aagaatctgg tgatatagct ggaactccag
    18481 gtgttaaatt agtaggacct gctggagaag ttgaaatgaa agaaggagtt atagttgctt
    18541 ctagacatat acatatgagc aatgaagatg ctgctaaatt tggtgtaact gataagcaaa
    18601 tagtaaaagt taaaacatct ggaccaagag cattagtatt tgaaaatgta ttagtaagag
    18661 ctagtgatag ctttaatcta gaaatgcatg ttgatatgga agagggaaat gcagcaggcg
    18721 ttaaaaatgg agatttagta gaattaatag ttgattaata ttataaatag actttatgct
    18781 agtagatatt ttatttgcta gcatattttt aaatgtaata aatttctata ggataaattt
    18841 aatcgattag attaatttat aaaataaagg ggtattataa aaaatgtaat aaaaacttac
    18901 aaaaatatga tgtaatatgg taaaatt
//
LOCUS       DAMKPG010000075        18722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMKPG010000075 DAMKPG010000000
VERSION     DAMKPG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.079"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            100..1701
                     /locus_tag="PC621_002696"
     CDS             100..1701
                     /locus_tag="PC621_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF4109843.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1920..3509
                     /locus_tag="PC621_002697"
     CDS             1920..3509
                     /locus_tag="PC621_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HDF4109844.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            3735..6011
                     /gene="slpA"
                     /locus_tag="PC621_002698"
     CDS             3735..6011
                     /gene="slpA"
                     /locus_tag="PC621_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF4109845.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTLGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            6247..8592
                     /gene="secA"
                     /locus_tag="PC621_002699"
     CDS             6247..8592
                     /gene="secA"
                     /locus_tag="PC621_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF4109846.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            9007..10878
                     /locus_tag="PC621_002700"
     CDS             9007..10878
                     /locus_tag="PC621_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HDF4109847.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            10985..11692
                     /locus_tag="PC621_002701"
     CDS             10985..11692
                     /locus_tag="PC621_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF4109848.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            11725..13560
                     /locus_tag="PC621_002702"
     CDS             11725..13560
                     /locus_tag="PC621_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF4109849.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(13608..14003)
                     /locus_tag="PC621_002703"
     CDS             complement(13608..14003)
                     /locus_tag="PC621_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF4109850.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            14230..16641
                     /locus_tag="PC621_002704"
     CDS             14230..16641
                     /locus_tag="PC621_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF4109851.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            16938..18515
                     /locus_tag="PC621_002705"
     CDS             16938..18515
                     /locus_tag="PC621_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF4109852.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         7752 a         2072 c         3469 g         5429 t
ORIGIN      
        1 ttcatatcat atatactcta taatatatgg aaaataaaga gaaaataaat agaaaataat
       61 cacaatataa atataaaata taaaaatcat cagggggata tgaaaatgaa caaaaaaata
      121 ttatcattag gtctagcagt atcattaatc ttagtaaact tcaaaagtgt aaatgcatca
      181 tcagtagtag aaaaaatata cggtaaagat agatatgaaa cagcagcaaa gatagctgat
      241 agacagactt atgaaacagt aattttagta aatacagaaa aatcacttgc agatggatta
      301 agtgcaagtg gattatcggg agctacaaaa gctccaatac tatttacaca acaaaataag
      361 ataccagcag acacaaatag atgtctaaaa aatatcaaaa aagcatatat aataggtaca
      421 gaagatacta taagtaaatc agtagaaaaa gaactagatt ctaaaaatat agaagtcaaa
      481 agaattggtg gagaagatag actaaaaaca agctatttaa tagctaaaga aatagcaact
      541 ataaaaaaag tggataaagt actattaact aacgcatata gtggagaagc agacgcaatg
      601 agtgtatcat cagtagctac tagagatgga gctccaatca tacttacaga tggaaagagt
      661 gtgccttttg atgtaaaaaa tattcaatca tattgtatag gttcagagga gataatgagt
      721 aaccctctag ttaaaaatac aaactcagta agaatagaag gaactgaccg atttgaaact
      781 aacaaaaatg taatagacta cttttttaat agtgcagatg gattctatgt atcagatgga
      841 taccaattag tagatgcaat agcagctgca ccacttacta aaaactctcc aatggtacta
      901 gtaaatgatg gaagcgataa aactgtatta gaaggagcta agaatataac ttctgtaggt
      961 gaaataaatg agaaggtaat acaacagtgt ataaatgctt caaagtcaaa tggacaacca
     1021 ccaacaatta cagttggaag tacagagata tataagggtg aaaagtttga cactggcaag
     1081 ttaaacatag tggctaaaga taacactggg aaggtattac caatagaagt tgatgggttt
     1141 atagatacaa atagagtagg tacatacata ttgacattaa aagctacaga cgaatgggga
     1201 aaaagtacag gaaaaagagt agaaataaaa gtgttggatg ataaatccta tgattacaac
     1261 agcccagaat ttaaaaaaat ggtatctact gaaatgtata acttaatcaa ttcctataga
     1321 aaagaaaaag gcaaagagcc tttggtagta tctagtaggc tagaaggtat ggcaaatgca
     1381 tggtctaagt atatgatgga taaaaaagta tttgcacatt atatagatgg aaaaaatgct
     1441 ccacaagtat tttctgagtt tggaatgaga agtgaggaaa atatagcata tatttatata
     1501 gattctaaga acgttcaaac gacacaagat gcaaaaaact tggcaaaggc tatatttgaa
     1561 gtgtggaaga agtctccaga gtacaatgca aacatgctta gtgatgagtt ctatagtact
     1621 ggatttgggc tttatatatt atctgatggt caagtgcatg ctactcagga gtttttaaat
     1681 ggcaatgaag gtagtttgta aaaaattcca taaatcttgt ttttttgtta atttactgat
     1741 attataaaat gaaaatcaca tctaaagtac tttttgtaat ctgttttgtt attgctttac
     1801 aggctatggt attttaggtg ttttttgttt tagtagttta tagattttga gggaatcact
     1861 actgaggatg taatttatga ttagctgaat tatataaaaa aattagtagg aggagttcga
     1921 tgaaggtaaa taagagagta ttatcaatag gattgacaat atcattaata atggcaggag
     1981 caccaaatat caatgcactt tcgagcatag aaaagataca agggaaagat agatatgaga
     2041 cagctgccaa aattgcacag aaacaaacat atgaaaatgt agtattagta aatacagata
     2101 atacactagc tgatggatta agtgcaagtg gattagcagg aacagttaaa gcacctatat
     2161 tattatcaca aagaaacagt ataccatcag acacagaaaa aatgttaaaa gatgtaaaga
     2221 aggtttatat aataggaact gaagattcaa taggaaaatc agttgaaaat gaattaaagc
     2281 aaaaaggaat agaagttaaa agaataggtg gtaatgatag aattgaaaca agttatctta
     2341 tagcaaaaga aatagcttca ataaaaccta tagataaggt ttttataact aatggttata
     2401 caggtgaagc agatgctatg agtgcgtcat cagtagcatc aagagatgga tcacctataa
     2461 ttttaactaa tggtaaaaat gttccatttg agaaaaaaga aggtgtgcaa tgttatgctc
     2521 ttggttcaga ggagataata agtaatgatt tagttaaaaa aactaatgca gttagattag
     2581 ctggagaaga tagatttgag actaataaaa aagttataaa acatttttat agttcggcaa
     2641 aagaatttta tctttctaaa ggttatcaat tagtagatgc agttgctggt tcttctatag
     2701 caaaaaatgc acctattgta ttagttgacg gaaatagtga taagtctgta cttagaagtg
     2761 ctgataagat tacagcacta ggaggaatag atgaaaagac actagaacaa tgtttaagtg
     2821 catcatcact ggatgcaagt gcaccaacaa taactgttgg aaatttaaat atttatcaag
     2881 gtgataagtt tgatataagt aaactgaata tcgtagcaaa ggatagcaat ggtaacgatt
     2941 tgacaccaga gcttatagga aatattgata ctgataaggt gggtaaatac aaagttacta
     3001 taaaagctac tgatattggt ggaaagacta catcaataag tgtagaagta aatgtacttg
     3061 aatataagac taatgatatg aatagtagtg agtttaaaag aatggtttct agtgaaatgt
     3121 atagtttggt aaattcatat agaaaagaaa aaggtaaaga gccattacaa gtttctaaaa
     3181 acttagaagg actttctaat tcctggtcta agtacatggc agataaaggt cagttttcac
     3241 atgtaataaa tggtaagaaa tctgttgaag tatttactgg atatggcttg agaagtgaag
     3301 aaaatatagc ttttgtacca cttgttacaa aaagtactta tacaacaaag gatgctagag
     3361 aagtagcaaa tgtaatattt acagtttgga aaaagtcaga taaatataat gaaaatatgt
     3421 taaatagtga ttttgtatat actgggtttg gattatatat attgcctaat ggagaagtgt
     3481 atgctacaca agaattttta aataagtaat ttaaataagt aaaatcaaat tttttaagtt
     3541 attaaatatt ggagtttaag ggtataaaaa ctcatatgct tgggaaaaat aataatttca
     3601 aaaaaatgac ttgcaaaaag aataaaaatg gattattata gagatgtgag aaatattagg
     3661 aatatatgga tgattattct atgtacataa tgaagagatg taattttaat ataatgttgg
     3721 gaggaattta agaaatgaat aagaagaata tagcaatagc tatgtcagga ttaacagtat
     3781 tagcttctgc agcacctgtg tttgcagcag aagatatgtc gaaagttgag actggtgatc
     3841 aaggatatac agtagtacag agcaagtata agaaagcagt tgaacaatta caaaaagggt
     3901 tattagatgg aagtataaca gagattaaaa ttttctttga gggaacttta gcatctacta
     3961 taaaagtagg agctgagctt agtgcagaag atgcaagtaa attattgttt acacaagtag
     4021 ataataaatt agacaattta ggtgatgggg attatgtaga tttcttaata agctctccag
     4081 cagagggaga taaagtaact acaagtaaac ttgttgcatt aaaaaattta acaggtggaa
     4141 ctagtgcaat aaaagtagct acaagtagta ttattggtga agtcgaaaat gctggtactc
     4201 tgggagcaaa aaatacagct ccaagtagtg ctgcagttat gtctatgtca gatgtatttg
     4261 atacagcttt tacagattca actgaaactg ctgtgaaact tactataaaa gatgctatga
     4321 aaactaaaaa gtttggttta gttgatggaa ctacttattc aacaggtctt caatttgcag
     4381 atggaaaaac agaaaaaatt gttaaattag gagatagtga tactataaat ttagccaaag
     4441 aattaataat aacacctgca agtgcaaatg atcaagctgc gactattgag tttgctaaac
     4501 caacaacaca atctggaagc ccagtaataa ctaaacttag aatattgaat gcaaaagaag
     4561 agacaataga tattgatgct agttctagta aaacagcaca agatttagct aaaaaatatg
     4621 tatttaataa aacagattta aatactcttt acagagtatt aaatggggat gaagcagata
     4681 ctaatagatt agtagaagaa gttagtggaa aatatcaagt ggttctttat ccagaaggaa
     4741 aaagagttac aactaagagt gctgcaaagg cttcaattgc tgatgaaaat tcaccagtta
     4801 aattaactct taagtcagat aagaagaaag acttaaaaga ttatgtggat gatttaagaa
     4861 catataataa tggatattca aatgctatag aagtagcagg agaagataga atagaaactg
     4921 caatagcatt aagtcaaaaa tattataact ctgatgatga aaatgctata tttagagatt
     4981 cagttgataa tgtagtattg gttggaggaa atgcaatagt tgatggactt gtagcttctc
     5041 ctttagcttc tgaaaagaaa gctcctttat tattaacttc aaaagataaa ttagattcaa
     5101 gcgtaaaagc tgaaataaag agagttatga atataaagag tacaacaggt ataaatactt
     5161 caaagaaagt ttatttagct ggtggagtta attctatatc taaagaagta gaaaatgaat
     5221 taaaagatat gggacttaaa gttacaagat tagcaggaga tgatagatat gaaacttctc
     5281 taaaaatagc tgatgaagta ggtcttgata atgataaagc atttgtagtt ggaggaacag
     5341 gattagcaga tgccatgagt atagctccag ttgcatctca attaagaaat gctaatggta
     5401 aaatggattt agctgatggt gatgctacac caatagtagt tgtagatgga aaagctaaaa
     5461 ctataaatga tgatgtaaaa gatttcttag atgattcaca agttgatata ataggtggag
     5521 aaaacagtgt atctaaagat gttgaaaatg caatagatga tgctacaggt aaatctccag
     5581 atagatatag tggagatgat agacaagcaa ctaatgcaaa agttataaaa gaatcttctt
     5641 attatcaaga taacttaaat aatgataaaa aagtagttaa tttctttgta gctaaagatg
     5701 gttctactaa agaagatcaa ttagttgatg ctttagcagc agctccagtt gcagcaaact
     5761 ttggtgtaac tcttaattct gatggtaagc cagtagataa agatggtaaa gtattaactg
     5821 gttctgataa tgataaaaat aaattagtat ctccagcacc tatagtatta gctactgatt
     5881 ctttatcttc agatcaaagt gtatctataa gtaaagttct tgataaagat aatggagaaa
     5941 acttagttca agttggtaaa ggtatagcta cttcagttat aaataaatta aaagatttat
     6001 taagtatgta atataaatat aataatataa aaaggcttct tatatgagaa gtctttttat
     6061 attatgtttt cgaatacaaa aaaagagact aaatttagtc tcttttttat gtgagaaata
     6121 cttaaatata taaagatgta atttatttaa gtatgattat tatactatac aataaaataa
     6181 ttgtctacta ttctataatt ttaaggtata ataataattg tatgataaaa gaggaggtca
     6241 aaaaatatgt cagttttaga tgcaatctta gataaagcgg atgaacaaga aattaaaaaa
     6301 ttaaatttaa tagtagataa aatagaatct ttagaaaaag atatggaaat caaagagaat
     6361 gaacaattaa aagaaatgac aaatactttt agacttagat tagacaaagg tgaaagtttg
     6421 gatgatatac ttccagaagc atttgctgtt gttagagagg tctcaaaaag agttttagga
     6481 atgcgtcaat ataaagttca gttaattgga ggtatagtaa ttcatcaagg aaagatagct
     6541 gagatgaaga ctggtgaggg gaaaacttta gttgaggtag ctccagtata tctaaatgct
     6601 ttaacaggaa aaggtgtaca tgtaattaca gtaaatgatt atttggcaga acgtgataaa
     6661 gaacttatgc gtccagtata tgaattttta ggattgactg taggtgtaat tttatctaac
     6721 caagacccaa gtataagaaa agaacaatat aaatgtgata taacttatgg aacaaatagt
     6781 gaatttggat ttgactattt aagagataat atggtgccag atttgtctca taaagtacaa
     6841 agggaactaa actttgccat agtagatgag atagactcaa tattaataga tgaagctaga
     6901 actccactta ttattgcagg agatggtgat gaagatttaa aactttatga gttagcaaat
     6961 aactttgtaa aaactgttaa agaagaagac tttgagatgg atagaaaaga taaaactata
     7021 gcattaacag caagtggtat aagcaaagct gagtcatttt ttggtataac aaaccttact
     7081 gatataaaga atatagaatt atatcatcat ataaatcaag ctttaagagg tcataagctt
     7141 atggaaaaag atgttgacta tgttatctca aatggagaag taatgatagt tgacgaattt
     7201 acaggaagag taatggatgg tagaagatat acagatggac ttcaccaagc tatagaagcc
     7261 aaagaaggtg ttgagataaa gaatgaatct aaaactatgg ctactgtgac ttatcaaaat
     7321 ttcttcagac tatatgaaaa actttctggt atgactggta ctgcaaagac agaagaaggg
     7381 gaatttgagt caatctataa attaaatgtt gtccaaatac caactaatag accagttatt
     7441 agagctgatt tgcatgataa ggtatttaaa acagaagaag aaaagtatag tgctgttgta
     7501 gaagaaataa taaggataca taagactaga cagccaatac tcgtgggaac agtttctgtt
     7561 gaaaaatctg agaagttatc taaaatgctt aagaagcaag gtattaagca tcaagtctta
     7621 aatgctaaac aacatgataa agaggcagag gtaatttcta aagctggtaa attagatgct
     7681 ataacgattg ctacaaatat ggcaggtaga ggtacggata tttctctagg tgcaggagat
     7741 aaagaagaag aagaaaaagt taaaaattta ggtggtcttt atgttatagg aacagaaaga
     7801 cacgaatcaa gaagaattga caatcagctt agagggcgtt ctggtcgtca aggagaccca
     7861 ggtacatcaa gattttttgt aagtcttgaa gatgatgtaa taaagcttta tggtggaaaa
     7921 actatagaga aacttatgaa gagaacaagt tcaaaggaaa atacggctat tgaaagtaaa
     7981 gcacttacaa gggctataga aagagcacaa aaaggtgtag aaggtaagaa ttttgaaata
     8041 agaaaaaatg ttcttaaata tgatgatact attaatgagc aaagaaaggt tatatataat
     8101 gaaagaaata aagtgttaaa tgatgaagat ataaaagaag atattcaaaa aatggttaaa
     8161 gacatcatac aagaagcagg agaaacttat ttaattggaa gaaaaagaga ttattatgga
     8221 tattttaaac atttatatag tacatttatg cctgcagata cattactaat acccggtgta
     8281 gataaaaaaa gtgtccaaga gataattgat agcacatatg aaatttcaaa aagggtttat
     8341 gacttgaaaa agatgatgat tggtattgat aaagttgcgg agttagaaaa aacagtactt
     8401 ttaaaagtgg ttgatcaata ctggatagat catatagatg ctatggaaca gttaagacag
     8461 tatataggtc ttaaatctta tgctcaaaaa gatccgttta aagaatatgc tttagaagga
     8521 tatgatatgt ttgaagcttt aaataaaaat ataagggaag caacagtaca atacttatat
     8581 aaatttaact aaaaaagttc atgtaatttt tattaaatga aaaataaaat attttgattc
     8641 tttatattgt aattacaatt aaataattaa ggaatacaaa tgaaaaagaa gctgaaaatc
     8701 agcttctttt tctgtgtgag aaatacggaa aaaagagatg cttgaaattt ctttcttcca
     8761 tttaatatta tactataaag aagatgaaaa gtctattagt tataaactgg attattaaaa
     8821 aaaattacaa ttttgttact tttttgtata cataaatatt aaaaaaataa aatataagaa
     8881 aaagtttata tcttttggtt aattattaca ataagtctca tttattgaaa taatatcaaa
     8941 tatatattat aattggtaaa taaggaaaaa ataataaaaa tttgaatttt ttagggggaa
     9001 aataccatga ataaaaaaaa tctttctgta attatggctg ctgcaatgat aagtacatca
     9061 gtagctccag tttttgctgc agaaactaca caggtaaaaa aagaaacaat aactaagaaa
     9121 gaagctacag aactagtttc gaaagttaga gatttaatgt ctcaaaagta tactggtggt
     9181 tctcaagttg gacaaccaat atatgaaata aaagttggcg agactttatc aaaattaaaa
     9241 ataataacta atatagatga attagagaaa ttagtaaatg ctttgggaga aaataaagaa
     9301 cttattgtaa ctataacaga taaagggcat ataacaaata gtgcaaatga agtagttgca
     9361 gaagcaactg aaaaatatga aaattcagca gacctttccg ctgaggctaa ttctataaca
     9421 gaaaaagcta aaactgaaac taatggaatt tataaagttg cagatgtaaa agcttcatat
     9481 gatagtgcta aagataagtt agttataact ttaagagata aaacagacac agtaacttct
     9541 aaaactatag agataggtat tggtgatgaa aaaattgatt taacagcaaa tccagttgat
     9601 tcaacgggaa caaacttaga cccttctaca gaaggattta gagtaaataa aatcgttaaa
     9661 ctaggtgtag caggagctaa aaatattgat gatgtccaat tagctgaaat aactataaaa
     9721 aatagtgacc taaatacagt ttcaccacaa gatttatatg atggatatag attaactgtt
     9781 aaaggtaata tggtagcaaa tggaacatca aagtcaatta gtgatatttc atcaaaagat
     9841 tcagaaacag gaaagtataa atttactatt aagtatactg atgcatctgg aaaagcaata
     9901 gagcttactg tagaaagtac taatgaaaaa gatttaaaag atgccaaagc tgcattagaa
     9961 ggtaattcaa aggttaaatt gatagctgga gatgatagat atgcaactgc agtggctata
    10021 gcaaaacaaa caaaatatac tgacaatata gttatagtta attcaaataa actagttgat
    10081 ggattagcag ctacaccact tgctcaatct aaaaaagcac ctatattatt agcatccgat
    10141 aatgaaatac caaaagtaac tttagattat ataaaagata taattaagaa aagcccatca
    10201 gctaaaatat atatagtagg tggagaatca gcagtatcaa atacagctaa aaagcaatta
    10261 gaatcagtaa ctaagaatgt tgaaagacta gctggagatg atagacatat gacttctgta
    10321 gcagtagcaa aagctatggg gtcttttaaa gatgcatttg tagtaggtgc gaaaggggag
    10381 gctgatgcta tgagtatagc tgccaaagct gctgaactta aggctcctat aatagtaaat
    10441 ggctggaatg atctttcagc agacgctatc aaattgatgg atggaaaaga gattggtata
    10501 gttggtggtt ctaacaatgt atctagtcaa attgaaaatc aacttgctga tgttgataaa
    10561 gatagaaaag ttcaaagagt tgaaggagaa acaagacacg atactaatgc taaggttata
    10621 gaaacatatt atggaaaatt agataaacta tatatagcaa aagatggata tggaaataat
    10681 ggtatgctag tagatgcatt ggcagcagga cctctagcag caggtaaagg tccaatactt
    10741 ctagctaaag ctgatataac agactcacaa aggaatgcac ttagtaaaaa attaaatctt
    10801 ggtgcagaag taactcaaat aggtaatgga gttgaattga cagtaataca aaagatagct
    10861 aaaatactag gttggtaata acttatactg tggagaagat tccttaatta atacaggaat
    10921 ctttttctat aattatgcta tttattgtaa attataacat tttataaagt aggaggatgt
    10981 agtagtggta gatgcaactt tattttttac accacatcaa gatgatgaaa ccttaagtat
    11041 gggaagtgct ataattgaac atgttgaaaa aagtgataca catgtcatat tatgcacaga
    11101 tggtagtaaa tctataataa gaaaagtttt ggatgatgga gggtcatgtt cttatcatat
    11161 taaagatgtt cataaatact ctttatcaga aagtgaattt tcaaaagata gagatgaaga
    11221 atttaaggat agctgtgaag caatgggtgt aaaagaaagt aatatacata tagaagataa
    11281 tagagcacat gatggtgagt taagtaaaga aaaggcaagg gaaataatac ttaagtactt
    11341 agaagaatat ccagatgcaa aagtaaaaac tgttactccg tttaaagcta gtggaataca
    11401 tgaagaccat agggcattag gagaagctgc actagagtta tataaagaag gcaaaataaa
    11461 agatttaaga ttttatgtag aaccatacga ctataaggat tttaaaaaag ttaatccaaa
    11521 tgtagaggta tggaaggtat tacctagtca agaggagaag ttattaagtg caatgaatgc
    11581 atataaaaaa tggaatcctg aaagtggaca ttatgctata ggatatcatt cagtaaaatc
    11641 tcattttgat gagctagcta taaataaaat acagtatgta catgctccat aaaatattat
    11701 gaaagaatta ggaggtaaga attaatgaaa atatcaaaaa agatagtgtc tttgttaact
    11761 atgacatttt taactgttac attatatgga aatacatcta atgcatctac aaaagataca
    11821 ttaacaggtt ctggaagatg ggaaacagca ataaaaataa gtcaagcagg atggaaaaag
    11881 tctgaaaacg ctgtgctagt aaatgataat tcaatagcag atgctttatc agctactcca
    11941 tttgctaaag caaaagatgc accaatatta ttaactcaaa gtaataaatt ggatagtaga
    12001 acaaaagcag aattaaaaag acttggtgtg aaaaatgtat atttaatagg tggttcaatt
    12061 gcattaagtt cagagattga aaagcagtta aatgcagaaa atataagttt tgaaagaata
    12121 tctggaaata gtagatatga tacttcttta aaattagctg aaaagctaga tagggaaaaa
    12181 tctatatcta aaatagtagt agtaaatgga gaaaagggtc ttgctgatgc agtgagtgtt
    12241 ggagctatag ctgctcaaga aaacatgcca ataatacttt ctgattcaga gaatggaact
    12301 gaagtagctg ataattttat agatagtaaa gatatagaaa aatcgtatgt aataggtggt
    12361 acatattcta tttctaattc tgtagaaaga agtttaccaa atgcaacaag aatagcaggt
    12421 agtagtagaa gtgagacaaa tgcaaaaatt atagaagaat tttacaaaga tactgatata
    12481 aaaaatattt atgtaacaaa ggatggtaca aggagtaagc atgatttaat agattcttta
    12541 gctgtgggtg tattagcttc taaaaatggt tctccaatac ttttagcagg aaataagcta
    12601 gatagttctc aaaaagatgt gttaaacact aaaattatag ataaagttat tcaaattggc
    12661 ggattgggta atgaaaatgt tgtagaagat gtaatagata tgcaagaaga aactaagtat
    12721 actgttgaaa ctattgatga acttaatgtt gctataaaaa aatcagatgc aaatgacatc
    12781 ataagattta agccagaaaa agataaaaat ataactaatt cttttaaaat agaaacaaat
    12841 aaagctataa ctattgaatt tgatggtata tataaacaat caataactat agatatgcca
    12901 aatggagata taaaaaactt tggggacata gataatgatc tagtaataaa taatataaaa
    12961 aatgagactt tagttaacga aggaagtata caagggatag atgtttatga tgagaatgga
    13021 tgtaaaatag agaatgagga ttctggagaa atatggctta taacgatact taaaaattca
    13081 aaagatgtat atattttgaa tcgtggagaa ataactaaaa tatctaataa ttcttctagt
    13141 gtaacaataa aaaattcagg taatattaat actataagtg gaagtgaaga accatcaata
    13201 agtggtaaaa aacctataac taatgataca gataaagatg aagataaaaa ggcacgtggt
    13261 ttatatccta aagtagaatc ttgtatacct gctttaaaag attatgtaag aataactata
    13321 tcaacaaaac cagagagttc aaagtataaa atttattata gagtagttga tgaaaaacca
    13381 tctgcgaaat atgttggaga aaaaattagt actaaaggtt ggagtatggt tacaatatca
    13441 aatcaatctt ttattgaaag agccaaagat ggatcatata tagaagcagt agaaatagat
    13501 acctcaaata atgaagtttc acgatgggga aaatctagca ttacagatga tggaatttag
    13561 acattaaact tattcatttt gcagattact atttagtaat ctgctattta ttttttaaaa
    13621 attatcatct tgctaaacaa gtaatttaaa actataacaa ctatattagt aaatattttt
    13681 acaataacat catttacaaa aagcaaatca atcattacat acataattaa catttcaacg
    13741 acaccagaaa gtagcctaaa actaacaaaa gacaaaaatt ctttaaatac gaaccttatt
    13801 tcaattcttt tactttcaaa tacaaaaaac ttattagtaa cataagcaaa taaaacagca
    13861 agtatccatg ccaaagcatt tgcaaccata aaattaaata aaataactct agtaaaaaat
    13921 aaatacgaaa ctatatttac aagagtagta aatgctccaa aaaataaata taatattgtc
    13981 tctctatgct tttttaaaat caaatataat cctcctaatc taatttaata taaatatact
    14041 taaagatact aacaaacatt agtatagcac atctattaat aaatcaatat atcttaaaat
    14101 attataaaaa taaaactata atattctata attaatatgt actcataatc catattttat
    14161 caaaataaaa tatgacctta atttagaaat ctgatataat aaaaattaaa tacatataag
    14221 ggggtaaaca tgagaaagta caaatcaaaa aaattgtcaa agctactggc attgttgaca
    14281 gtttgctttt taatagtgtc aacaatacct gtctcagcag aaaaccataa aactctagat
    14341 ggagtagaaa ctgcagagta ttctgaaagt tatcttcaat acctagaaga tgtcaaaaat
    14401 ggagacacag caaaatataa tggagtaata ccttttccac atgaaatgga aggtacaaca
    14461 cttcgaaata agggcagaag tagtcttcca tcagcatata aatcaagtgt agcatacaac
    14521 ccaatggatc ttggtcttac tacaccagca aaaaatcaag gaagtcttaa tacatgctgg
    14581 tctttttcag gtatgtcaac tttagaagca tatcttaaat taaagggata tggaacgtat
    14641 gacttatcag aagaacattt aagatggtgg gcaactggtg gaaaatatgg atggaactta
    14701 gatgatatgt caggtagttc aaatgtaaca gccataggat atctgacagc atgggcaggt
    14761 cctaagttag aaaaagatat accatataat cttaaatctg aggcacaagg tgcaactaaa
    14821 ccttcaaata tggatactgc acctactcaa tttaatgtaa cagatgttgt tcgtcttaat
    14881 aaagataagg aaactgtaaa aaatgctata atgcaatatg gttcagtgac atcaggttat
    14941 gcacattatt cgacatattt taataaagat gaaacagcat ataactgtac aaataagagg
    15001 gctccattaa atcacgctgt agcgatagta ggatgggatg ataattattc aaaagataac
    15061 tttgcatctg atgtaaaacc agaatcaaat ggagcatggt tagtaaaaag cagttgggga
    15121 gaatttaatt ctatgaaagg attcttctgg atttcttatg aagataaaac tctactaaca
    15181 gatacagata actatgcaat gaaatcagta tcgaaaccag atagtgataa aaaaatgtac
    15241 caacttgaat atgctggtct tagtaagata atgtcaaata aagtaacagc agcaaatgta
    15301 tttgatttta gcagagattc tgaaaaactt gactctgtta tgtttgaaac agactctgta
    15361 ggagcaaaat atgaagtata ttatgcacca gtagtaaatg gagttcctca aaacaactca
    15421 atgacaaaac ttgcaagtgg aacagtatca tattctggat acataaatgt acctactaat
    15481 tcttacagct taccaaaagg taaaggagca atagtagtag ttatagacaa cacagcaaat
    15541 cctaatagag aaaaatcaac tttagcatat gaaactgata tagatggata ctatctatat
    15601 gaggctaaag caaacttagg tgaaagctac atacttcaaa acaataagtt tgaagatata
    15661 aatacatata gtgaattttc tccttgtaac tttgttataa aagctataac aaaaacatct
    15721 tctggacaag ctacttcagg agaatcttta actggagcag atagatatga aacggcagtt
    15781 aaagttagtc aaaaaggatg gacttcttca caaaatgcag tattagtaaa tggagacgca
    15841 atagtggatg ctttaacagc tacaccattt acagcagcaa tcgactctcc aattctttta
    15901 acaggaaaag ataacttaga ttcaaaaact aaggcagagt tacaaagatt aggaactaaa
    15961 aaagtttatc taataggtgg agaaaattct ttaagcaaga atgtacaaac tcaacttagt
    16021 aatatgggta tatcagtaga aagaatttca ggtagtgata gatacaagac tagtatatct
    16081 ctagctcaaa agttaaatag tataaaatct gtttcacaag ttgcagtggc aaatggtgta
    16141 aatggacttg cagatgcaat aagtattggt gcagcagctg ctgataacaa tatgccaata
    16201 atacttacta atgaaaagag tgagttgcaa ggtgctgatg agtttttaaa ttcatcaaaa
    16261 ataactaagt cttatataat tggtggtaca gctactttat catcaaattt agaaagtaag
    16321 ctttcaaatc caacaagact tgcaggaagt aatagaaatg aaactaatgc taagataata
    16381 gataaattct accctagctc agatttgaaa tatgcttttg ttgttaaaga tggttcaaag
    16441 agtcaaggag acttgataga tggactagca gtaggagcat taggtgctaa aactgattca
    16501 ccagtagttc tagttggaaa taagttagat gaaagtcaaa aaaatgtact taagtctaag
    16561 aaaatagaaa ctcctattag agttggtgga aatggaaatg aaagtgcttt taatgaacta
    16621 aatactcttt taggaaaata gtgaatggaa agatagtaag gatatacatt ttattattaa
    16681 aaaatattca atagaagttt ataaatataa aataacaaaa aggggtgtct caaaatgaac
    16741 tttttagttc atgaggcact cttttttgtt gttttatatt tttatcattt aaataatgaa
    16801 aaataggctt gtctatattt ttagatagtt ttcttattat tgatgaaaaa tgtaataatt
    16861 ttaaactgta aaatagtgta aagttatttt ataatataaa tatagtaaga ttttaaacaa
    16921 aataaggggg aataataatg aaagcaccaa aaactatttt aacaatactg acaatagcgc
    16981 ttactttaag tagcatttct ataataccat catatgcact tacagaggaa aaattaatag
    17041 gtaatggtag atatgaaact gctgtaaaga taagtcaaaa ggcatacagt tctagttcta
    17101 atgtagtgtt agttaatgac aattcattag ctgatgcact ttcagctaca ccatttgcaa
    17161 aggctaaagg agcaccaata cttttgacag aaagtgataa gctagatgat aggactgaaa
    17221 aagagataaa acgtctagga gctaaagata tttatttgat aggaggtact gctgtactta
    17281 ataaagatat agagaacaag ttaaaaggta atggacttaa tgtagaaaga attaatggta
    17341 aggatagata tgaaacttca ctgattttgg ctaataagct taaggatata aaagatatta
    17401 aggaagttgc tgtagtaaat ggtgaaaaag gattaagtga tgctgttagt gttggagcac
    17461 cagcagctca aaataagatg cctataattt tgtctagtcc taaggacgga gtagaagcat
    17521 ttgataaatt tataagagat gagaaagtta taaaagcata cgtaattgga ggaacaaatt
    17581 ctgtttcaag agctgtagaa aagagccttc ctaatgcaga aagagtgagt ggaaaagata
    17641 gaaatgagac taatgccaaa gttatagaaa agttttatac tgatacaaat ttaagtaatt
    17701 tatatgtaac taaggatggt agtaaaaatg agaatcaact aatagactcg ttagctgttg
    17761 gagtattggc tgcaaaaaat gagtctcctg ttgttttggt tggtaataaa ttaaacacga
    17821 aacaaaggga tatacttagt actaaaaaat taaatactat aacacaggta ggtggaaatg
    17881 gaaatgaaga agcttttgat gaaattaaga gtttacaaga aaaaactgtg tttgaagcaa
    17941 aaactgttga ggaattaact gatatgataa atatagcaag tcctaatgat attataaact
    18001 ttaaaccaaa agagaatact gtaaatgaag cttttagaat ggtaacaaat aaaccaataa
    18061 ctgtgaatat aaagggtgat tgttcaaaga ctcttacagt agatatgcct aatggtgaag
    18121 tgaataacta tgctactttg gtaaatgtta tagttagaaa tattggagaa ggtggattta
    18181 ataaccatga tacaatcact atattgtcag ttcgtgacaa aaatggtaga gttatagaaa
    18241 atactagaaa ttcagatata gatacgttga tgatattagc ttctgctaat gatacaaaat
    18301 tgattaatga tggatatata ggaaagctta tagataattc atctaatagt gatataacta
    18361 ataatggtac tatagataaa aaagttaatc aagttgagaa tttagaggct aaagtagatt
    18421 caattgaaaa agctatagat tctataagtc aaaaagtcaa taaaatacaa gatatactag
    18481 ataagattgg atttctaaag aaatttctta gttaagaata tgtatattta agtgaagtgt
    18541 gtgttgtaat tgttctaaag ttatatagtt aaacaggttt atatacaaac agacttatag
    18601 ataaatatgt agtttagtat atatttgaat aagctttggg gaggtaacat ggggattgga
    18661 attgaatgta acaaaaaaat aaataaaagt attgacaaaa ctagaataaa gaaaaataat
    18721 ct
//
LOCUS       DAMKPG010000076        17791 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMKPG010000076 DAMKPG010000000
VERSION     DAMKPG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.080"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(68..1672)
                     /gene="bclA2"
                     /locus_tag="PC621_002706"
     CDS             complement(68..1672)
                     /gene="bclA2"
                     /locus_tag="PC621_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF4109853.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGAVGA
                     TGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGVAGAT
                     GATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATGATGATGANGATG
                     PTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFPTSSTINLAVGGI
                     YNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSVSLSFLVDARAAA
                     VTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2050..2589)
                     /gene="hpt"
                     /locus_tag="PC621_002707"
     CDS             complement(2050..2589)
                     /gene="hpt"
                     /locus_tag="PC621_002707"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF4109854.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(2917..4224)
                     /locus_tag="PC621_002708"
     CDS             complement(2917..4224)
                     /locus_tag="PC621_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF4109855.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4649..6424)
                     /locus_tag="PC621_002709"
     CDS             complement(4649..6424)
                     /locus_tag="PC621_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109856.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6421..6558)
                     /locus_tag="PC621_002710"
     CDS             complement(6421..6558)
                     /locus_tag="PC621_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109857.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6636..7205)
                     /locus_tag="PC621_002711"
     CDS             complement(6636..7205)
                     /locus_tag="PC621_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF4109858.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7389..8024)
                     /locus_tag="PC621_002712"
     CDS             complement(7389..8024)
                     /locus_tag="PC621_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF4109859.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(8692..9573)
                     /locus_tag="PC621_002713"
     CDS             complement(8692..9573)
                     /locus_tag="PC621_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF4109860.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(9693..10700)
                     /locus_tag="PC621_002714"
     CDS             complement(9693..10700)
                     /locus_tag="PC621_002714"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF4109861.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(10720..11202)
                     /locus_tag="PC621_002715"
     CDS             complement(10720..11202)
                     /locus_tag="PC621_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF4109862.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(11220..11687)
                     /locus_tag="PC621_002716"
     CDS             complement(11220..11687)
                     /locus_tag="PC621_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF4109863.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(11702..12460)
                     /gene="prdD"
                     /locus_tag="PC621_002717"
     CDS             complement(11702..12460)
                     /gene="prdD"
                     /locus_tag="PC621_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF4109864.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(12540..13265)
                     /gene="prdB"
                     /locus_tag="PC621_002718"
     CDS             complement(12540..13265)
                     /gene="prdB"
                     /locus_tag="PC621_002718"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12813..12815),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF4109865.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(13303..13587)
                     /locus_tag="PC621_002719"
     CDS             complement(13303..13587)
                     /locus_tag="PC621_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109866.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(13668..15548)
                     /gene="prdA"
                     /locus_tag="PC621_002720"
     CDS             complement(13668..15548)
                     /gene="prdA"
                     /locus_tag="PC621_002720"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF4109867.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(15977..17737)
                     /gene="prdR"
                     /locus_tag="PC621_002721"
     CDS             complement(15977..17737)
                     /gene="prdR"
                     /locus_tag="PC621_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF4109868.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
BASE COUNT         5098 a         3540 c         2109 g         7044 t
ORIGIN      
        1 tattatccta tttattggta tatgaaatca tattgtttat tattttataa tcttttcatc
       61 atataaacta ttgtattcta taaactgata catacccagc tggagaagta cctgtcgtgc
      121 ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact aaaaagctta
      181 aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata aagttatttt
      241 gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa agtatggcac
      301 gtataccgaa agatacattg tatatccctc caactgcaag atttatagta ctacttgttg
      361 gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat gtattattcc
      421 ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact cctgttgctc
      481 cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc gcccctgttg
      541 ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct gctactccat
      601 ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc cctgttgctc
      661 cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt ggacctattg
      721 gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct gttgctccat
      781 ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct cccgttgggc
      841 ctgtcgctcc tactgctccg gtcgctcctg ttggacctac caaaccattt gctccggttg
      901 ctccggttgg gcctgttgga cctaccaaac catttgctcc ggttgctccg gttgggcctg
      961 ttgggcctgt tggacctacc aaaccatctg gacctgttgc tcctactgct ccggttggac
     1021 ctgttgctcc tattggacct gtggcacctg ttactccatt tgctcccgtt gctcctatac
     1081 ttccggttgg acctgttgct cctgtatttc ctgttgggcc tgtagcacct gttactccat
     1141 ttgctcccgt tgctcctgtt gctcctgttg gacctgttat cccattcgct cccgttgccc
     1201 cagttgctcc ggttagtccg gttgcaccat ttgctcccgt tgctcctgta tttcctgttg
     1261 gacctgttat tccatttgct cccgttgctc ctgttgctcc tgttggacct gttatcccat
     1321 tcgctcccgt tgctcccttg tttccggttg gacctgttat tccatttgct cccgttgctc
     1381 ctgttgctcc tgttggacct gttattccat ttgctccagt cgctcctgta tttcctgttg
     1441 gtcctgtaga acctgttgtt ccatttgctc cagtcgctcc catatttcct gttggtcctg
     1501 ttattccatt cgccccggtt gctcccgtat ttcctgttgg tcctgttatt ccatttgctc
     1561 cggtcgctcc ggttgcgcct ctaggccccg tcggtcctgt tggaccagta gcacctgttg
     1621 gccctgttgg acctacatct tgatataaac ttggacctga aatatcactc atataattaa
     1681 tcctcctttt ttaaagttag agtattactc tataaatatt gagtattact ctatataatt
     1741 atatgaatcg acaatttctt atgtgataat gtatctattt gcttatatca acatatatgc
     1801 tttgattctt aaatttcatt acttttttgt aaaatctttt acaattatta gtactctttt
     1861 acaattatta atacaattaa aaaactcatt tcccatcaat tatttaaaat ttttttatta
     1921 tgttataaaa aatagataat taaattgcat aaaattaatt taatactact ttaattactt
     1981 tttaaataaa aaaacctcaa cacgattaat aaaaatcata ttaaggcatc cacaaaatta
     2041 tataattgct tatctatctt catcagtaac attgaatata taaggaacat ttctataata
     2101 atcaccaaaa tcaaacccat atccaaccac aaacttatct tctatttcaa atccacaata
     2161 atctggcact aaatcaactt ttcttctgct tggtttatcc aaaagaacac agcattttaa
     2221 actagcagga tttttagctt ttagatgttt taacacaaaa tccatagtaa gtgcagaatc
     2281 agtaatatcg tctacaacca aaacatcata tcctgctata tcagtagtca catctgaaac
     2341 cactttaacc tttccagtac tttcttcatt gtttccataa ctagatgtag tcataaaatt
     2401 aactctaagt ggtaaattta tatttctaac taagtctgca caaaatataa agctaccttt
     2461 taatagagaa actaccataa gatttttatc tttataatct ttttctataa ttgctcctaa
     2521 ttcccttaat ttatctttaa ttttttcttc tgaatacaat acttcccatt tttttgtttc
     2581 tatatccata aaaagacctc caattgtaat aatccttctt aattttataa gagattgctc
     2641 actttttcaa tactcttatt caatattagt caatattatt gagtttttat gttcattcaa
     2701 caattattaa caatgtatct tatattttaa gtagttcata tatccacact aaattacata
     2761 ctatatattt tagacaaaaa aagaaaaaac ttgcactaac aagttttttc tattatacat
     2821 aaatttatac ataatcatat tttattagag ggtactaatt aaatttattt atgctatttt
     2881 tgttatacct cataatacaa aacatattat aaagtactag tcaactttat tcatatcatt
     2941 cattacattt aagattatct catctgttat agacatacct ttgtcactta ctctacctaa
     3001 gttttgtata ctttgctcaa cacttgaacc aactatacca tttagtggtg gtacaaaaca
     3061 atcttgctta gctattatag cactttgtat agctgcagat gctgcagaag ataattttaa
     3121 tgcacaacca gctttcgcac cgtcacatat cattccactt aagtcagcta tcatattttc
     3181 tatagctcct tctatttgct tttcagtacc attcataagc caagagatac cagcagttgc
     3241 tccagtagaa gcagcaacac cacatccaca cacagcagat aatcttcctg tatagttctt
     3301 aatataacca gttactagat gagatatagc taaagccttt gctagttttt catcagattg
     3361 tgggaatttt ttattataag caacgattgg aagaattgct gttaatccat gatttccact
     3421 tccatttgaa ctcataacag gcatttttac gcctgccatt cttgcatctg aagctccagc
     3481 agttaacatc attgcataat ttataacatc attacctaaa agaccttctt ctatagactt
     3541 tttaattcca tatccgacac caataccagt tttttgtttt aatccatatt cagccatctc
     3601 ttcattcatt tttactccat ctagtaaaaa ttctatatct ttaaaatcaa tttcttctac
     3661 attttttatt aattcttgta tagttactgt atccattaaa ctttcagctt tttctgccgc
     3721 atcatttgaa gctgatactt ttggttcatt gtctaataat acttctccat ctttttctaa
     3781 gaacgtaaaa ttatcatgtt tagttcttat tctcacttta gcaatatgat tttttccttt
     3841 taacacaact tctataaata ctttctctct tgtatcagca ggtgttattg atagtggagt
     3901 attatccata taatcctcag ctattttaac ttcttctttt gttagagttt ctaacacact
     3961 taatccattt tcagaatggc cacctactat acctagagct gctgcaattt ttaatcctaa
     4021 tctttcagta cctggtatac ctacgcacat gccatttttg tagatactag gactaactaa
     4081 catacgattt tcaactattt cttctcctaa taattccttt gcctttgcac atgctagagc
     4141 taaggctact ggttcagtac accctactgc tggtttaact tctgccttta acatttccac
     4201 taattctttt cttatatctc tcattatata taaacctccc aattaagttt agatttagta
     4261 ctcaaataat taattaaata atttcttatc tatatttata gcaaaataca tgccatattt
     4321 tttatatctt tttatatttt tagtcttata ttctataacc attggtatga ctgcttctat
     4381 ttatgtattt tttttattta catagagtat ttatattctt taaaatggag aaacatatga
     4441 caatttcgca aaaagacaaa aaaaagtctc agaatgagat tctatatcat tttgagacgg
     4501 ctcaaatgtg ttatttttta acttataaaa accatttttt aattcataaa aaaagattat
     4561 gcaaaacact tatagtataa gtttaaagct ttctttaaag ttatgaattt agaaaaattt
     4621 aaattttata tagcttttta ttatattact atgaaattat attgtatttt tcaatttttc
     4681 tatataatgt agctcttgat attccaagtg ctttagctgt taattcttta tcttttttat
     4741 aatctttgta cttatttaaa gcttttatta tctcaatctt ctctagttca tcaagtggaa
     4801 ttatcctatc gcttttaact tctaactctt ctacagaggt attcttaatt ttttgaggaa
     4861 ttacattcat tgtgattatt gaagaagaag acatattcac actatattca ataatattct
     4921 gcaactctct tatattccct ggccacttat attccatcat agcatcaaca accctgctat
     4981 ctatgtcaat aacattttta tctaattttt ttgaatactc tttaaccata tgcttgatta
     5041 taagtgggat atcttctttt ctctctctta aacttggtaa ttttataggt ataacattta
     5101 atctataata taaatcttct ctaaaagtgc cattttgaac cattttttct aaatctttat
     5161 ttgtagcagc aataattctt acatctattg agacatttga tttacttcca attttattaa
     5221 gttccttttc ttgtaatact ctcaataact ttgactgtaa atgcaaactc atatctccta
     5281 tttcatctaa aaatatactt cccttatgag ctacttcaaa cattccaagc tttccacctt
     5341 ttttagctcc agtaaaagct ccctcttcat atccaaataa ttcactctct aataaattgt
     5401 ctggtatagc agcacagttt acagctataa aaggattatc tactctattg ctatggttgt
     5461 gtatggctct tgcaaatagc tccttacccg ttccactttc cccagtaata agtactgttg
     5521 atgttgattt agaagctatt agggcttcct ttttagaatt tcttatagct acactttctc
     5581 caattatgtt ttccaatttt attctataat ctttattcat cttattgtag ttttttatag
     5641 catctctttt atctataaaa tcaataacat atccctttaa ctcatttttt aatactattt
     5701 tattgatgtt gtatattccc ttcgaatcat atccgtatct tttataaaaa aaactacacg
     5761 atttgtcttt cttaaagtta tttatagaag gtttctttat aaaatctaat tcgacaaata
     5821 tgtttttgcc agttatacta tcctttatat tgaaaagctc tttaaatttt gaattatatt
     5881 tatcaatttt tccatccaca tcaatagaca caacagcttt gtccatattg tttaatagtg
     5941 tctctaattt tttcttttct aattcaagct cataagtttt aatttgtgct tttaatttat
     6001 tagatattaa gtctgccatt ttgcctaaaa agtttataag accactttta ttattttcta
     6061 taatttgtgc ttgttctttt gtaaaggcta taaggcctat aacgccatga gaatcgccat
     6121 cacatataat aggacaacac acctctgcaa actctacaca agccccactt ttatagcatt
     6181 ccctacatac atcactactc tttgggtcat caattagtat tgtgatctgt tctgtaagtg
     6241 attttttaaa agccgtatat ttatctactt tctcacctat tttgtctata tatactcctg
     6301 tacctgcaat cctaaataac tccttattta ctatagttac atctatattg agtactgata
     6361 ctatagcttc agctatattt tgaatatcag attttatata ttctaaacct atcataacca
     6421 tcattcctta tttttcttta acataagtat ttgtcttatt tctttaagaa tcaagtatat
     6481 cttatttaaa gtaaattgga ttgacatcag gattacaaag agcataacaa ttattattaa
     6541 ctcataattt atttccatct aaacatctcc ttataacaaa agactaggct taacctagtc
     6601 ttttatatta tatccatatt tatattttaa ctcatttatc ttctttttcc cacataaata
     6661 tattttaatt tatttgactc tttaatcata ccatcaaagt ttaatcttga aaatatatta
     6721 aatacttcat gtggatagtg aatctttgca acagaaccac aaaatttatg tttaagataa
     6781 tttaacttaa cttttaaatt ctttaaattt aaatcctcta atataacctc tccattgccc
     6841 tttaacactc tttgtatttc agatgaagct ttttcacaat ctgtaataac attaaattgg
     6901 tcaactatta ttatcttgtt gaactgatta tcatcaaatg gtagaaactc taacttacct
     6961 ttaactacat tgcaattttc attatacact agagaataac agacatcttc ataattacta
     7021 ttctccagta tagttacaga agttccaaga cctctcaatc tcttgcctag gtttcctata
     7081 tgaccaacaa gcaatatttt atcacctaca ttatactcaa ttgtatccaa aaaacattct
     7141 aattcatcag atgtatattt agctttaact ggctgtttat ttatctgaca taatatgctt
     7201 ttcacaatgc ttcacctctt catttcctca attataaatt atgtacagat tacattataa
     7261 taaatggcga gcaactattc aagagatatt tcattagtat ttttttaaac aatctttata
     7321 cattttaata tgatattata ccaaatttat taggatttta ttcataaaaa tcagtctatt
     7381 ttttatgttt attcattgtt attttcatcc aattttttta ttttcgatat ggatacttca
     7441 tatgccattt ttttgaccac attattttca tctatttttt tctcatattc tctactttga
     7501 accctacccc aaagctgtat tctatcacca acctttagat tctttgcaaa tttagcattt
     7561 ctaccccaaa ctattgatgg tatataatca gatttattat aaggtctgtt tatagcaact
     7621 aataaatcgg ttatttccct tcctaaaggt gtcttcctat acacaggttc cttacataca
     7681 tatccatcta aaaatatact attagggtct ttattttcct cgtctatgct ttttatttct
     7741 ctgacaaaca ctgtaagcac taatctattt ttattattta atgttttgtt atatgatctt
     7801 aattggccta cgacattgac taaattttgt ttatcaaggt caatatcttg gagaagtctt
     7861 tctgatacag tcattggaag tatatcaaaa gaatcgctta gtctgtttat ttttattttc
     7921 atattataaa atttttctcc aaatatctca tggctaaatt ctagcttatt gtctaactcc
     7981 cctcttaagt ttactacgtt attctcatct ttaacagcta tcatatttta tccctccaca
     8041 attgttaatt cttatatttc tggtatcagc ttatataata aatatattta taagggatgt
     8101 attttattac aaatctttga ttgctgttta aaagttttta tttagttata aatttcattt
     8161 aatatttcac ttttataatt ataaaattat tatctttttg tactacatat actaattaaa
     8221 ctacctattt cagttttgta ttaattataa aaatcctatt atataattaa taagtcatat
     8281 ataacttaaa tttattcact ctatataagc aacttcaacg aattaaattt attcaaataa
     8341 ttcagatgaa tattgagtca ttataaactt atttttctta attataagtt atattagact
     8401 tattacagct tatattttta ttaatctgta ttaatatatg aacatttatt gaacttattg
     8461 attataaaat ttaaatattt agctttaaat tatgctttat tctttgaaaa tacaacgttt
     8521 tttacattaa atctactaaa tcactatata tattacataa ttggcatgtt aattgcttta
     8581 tattaattta gcaaataaaa aactgcccta aaatattaat tcaatatttt aaagcagttt
     8641 ataatcttta ttattagctg taatatttaa taaacactaa gtagtcttta actaagcagt
     8701 ctttttgttt ttatttgcag ccattatcat tgtagcagaa gtataagcta taactactat
     8761 tactaatgct tttaatatat ccattggtag agtttttact atatatgcag ctacgaaaac
     8821 tcctattaaa ccacatatag ctattataac tgatgctttt ttagtatatg catcttcttt
     8881 tataaatttc atacttgcaa ctggcattaa taatgcacaa gaacccataa ctattgggaa
     8941 tgcaactttt gcagacattc ctaagaaata aaccattgcc atacctggag catatagacc
     9001 tataccagct gtcattaaag ctcctagtat aaagttacct attacaccta ttaccaattt
     9061 aacaccagta agacctaatt catttcctcc acctggtaat acgttaatcc aaggatgacc
     9121 taataacatt aatattgctg ttaatgctaa agttacaccc ataacaagtt gtatttttct
     9181 ctcgtccatc ttagctatta ttcctgcacc tatatatgaa ccaactgctg ctgccacaac
     9241 catagatact aaagtaacac catcaactgg tatgattgat gtaaatatga atgcttctgt
     9301 aacaactgga atagtatgtg ctacgtttaa tgtaccaggc atttttttat ctggtatatt
     9361 taactttaaa gcctttgaca ttgctattgt tggagcaaat gaccctattc ctaatgtatc
     9421 aaagaagtct gttataaatc ctgttacagc aacttttgga gtactttgtt cttctagttt
     9481 cccctctttg tttgctttag ctacgtcttt accaaacaca actgcaaaga ttgctgcgaa
     9541 tactacatat aatgccttta agattgctac cataatccat cctcctattt ttttattttt
     9601 tcatatattc tgaatcttat agcctaaaag ctatagaata gcaaccgaca agtggctact
     9661 attctatttt aatttcctaa tttatttatt acttatttaa gaataaatcc atgtttaagt
     9721 gggtcttctt catctattac aaaatgatta aatccagtta tataagcaga accagttatt
     9781 ttaggtacta cagcattgaa atctgcaact ttagtttctt caactatttc acctttgaat
     9841 aaagttccta atatactttc atatacgaat ttttctccta cttttaattc acctttagca
     9901 tgtaaagtag ctaatttagc acttgttcca gttccacatg gagatctgtc aacttgacct
     9961 tgaccaaata taactacatt tttgtaagta gcttctggat gagttggctc atcatatatt
    10021 tcaactaagt ctacagtttt tatatgagct aaagttggat gttgtatttc tattttctca
    10081 tttattatat ctctaagttt catagctagt tcagttaatt taccagcatt ttgaggttcg
    10141 atttttaaac ctaattgact tgcatgtatt atagcaaaga agcttccacc aaatgatatg
    10201 tcaaatttaa cagtcccaac acctggtaag tcaacttcaa caccttcttt atataagaaa
    10261 gctggtacgt ttaagaatga tacttcttta gcttttccat ctacaactgt aacgtctcct
    10321 cttattattc ctgctggagc ttccataact acatgagtta caggctctac tgctggaact
    10381 acacctgttt ctatagctgc tgtcatagct cctattgtac catggccaca catgttaagg
    10441 tatccaccac catccatgaa gattattcca aagtcagcat ctgggcaaca aggttgagtc
    10501 attacagaac caaacatatc attatgtcct cttggctcta acattatagc agttcttaaa
    10561 taatctagat tttcctctaa atattctttt ttctcaggca tagaatttcc ttttatgtta
    10621 ggtattccac ctacaactat tctagttgct tcacctgctg tatgagagtc tatagcttgt
    10681 atacttctgc taaatttcat atcaaattcc cccttaggtt tattttgcgt ctttacgcat
    10741 ttttaatttt aggaaagcca atgctttttt aacatcaact tcatccatgt gattgtctcc
    10801 aactacttct gtttctatgt agcctgttgt ctttttataa tcaattatgg tagctttatc
    10861 caaatatgga ttggtaatta ctaatccatc tgctcccata atagtaagac ctactgcagg
    10921 tatatctgat tttacaaact ctgctatagc atttgtaaaa tcaatgtgct ggtttcctgc
    10981 catatctaaa gttataatca ctccatcagg tcgcaatgat tcaatccatc ttccaagtct
    11041 agttgctaaa aatgttttcc attcattgtg ctctgatgtc ccaactacaa ccactcctaa
    11101 caagtctatg tcagaatcat tatctatcaa atctagtaac gggtctctaa atatatgaag
    11161 tgatgtgttt ttcattgatg tagaaattcc cacaaaactc attcccttac ttaaattttt
    11221 tatccatgtt ttctcatttt aagttttaaa aatgctaaag cttttttagc atctatttca
    11281 tttgtgttgt tttctccaac tacttctgtc tcaatacctt cctttgattt gttcatatca
    11341 actatggtat ccatatattg attgcttaca acaaacttag cttgagtccc attaaatgta
    11401 actccaacta caggtatatc tctttttcct atttcctcaa atgtgttagc gtagtcaaca
    11461 tgtgagtttc cccaaccatc tgatgaaact atagctccat ctactctcat ggcttccagc
    11521 caagcggccg ttctttgtcc cacaaaggtt ttattttcat ttccatctgg agttcctact
    11581 actattactc caagtaaatc tacatcctta tcactttcaa ctatgtctaa aagtgggtct
    11641 ctaaagtgat gaagagatgt ttcttttgtt gaaggtccta ttcccataat atccacctct
    11701 cttaagtcat agctctaagg gcaccatctc tgtattcatt aggagatata atcataggta
    11761 catttcccat atctataatt gatgttcctc cttcaaatcc actaggttcc tttgagaata
    11821 actgattgtc atacatagca ccttgacctg ctatttgttt tactatgaca accttttttg
    11881 catttggtcg aattttgtca aaatattcac gaacttgagt agcatttctt ccgtcttcca
    11941 ttttcaaaga cgctcttatt tcttgtataa aatcatcaca tgctctgaat gctgcaagtg
    12001 gaagtgttct ttcaaaaggt aaacctccct ttaaagttac atctacatgg attatataat
    12061 cttcaactga tggagttcca tatctcccaa ataccatttg gtctttcaaa attccttctg
    12121 aagaaccaaa ttcatgcatt tgatttccat cttcatctgc accagttaac attacatata
    12181 cacccgttaa agtatgtgtg ataccctctc ccagtctgcc taaaactttt gttgatattg
    12241 gtataatgtc cataatagta ttaatttctc tatcataatc tccaggtttt ataatgtcta
    12301 cttttatgtc tgttattaac tcttctcttt cttttatttc atctatactt gataaatcta
    12361 ttgtaagtac atctttcttt atagaagttt ttgaaccgaa attaacttca ttcatatgaa
    12421 acggttttat aacaagtctt ctcattattt tttcttccat aatatccacc tttgttctta
    12481 aaacccctct cctaaccgtg taggagaagg gttctatatt ttcaaatata aattacattt
    12541 taaacgtgag ctttatattc gtatggtaat ggaactatct taccagcaga tggtattgca
    12601 actaattgct ctaatgtatc tcttaatata tgtgtttgca tttctatatt atgtggttca
    12661 ccagcattag cacccattgg aactaatgga gcaactgctc taggagttcc gttttgtcta
    12721 actactggag gaagagctgc tattattatt gtaggtattc cagcttcctc tattgctctc
    12781 tgcacgatca cggcagttct atggcaagta cctcagccag ctgtcataac tacaccgtct
    12841 actccctctt ccttaagttt agcagcgata gcaggaccag tttcttcagt gaagacatgt
    12901 tggtttcctc caccacccat gaatgcatag tgcataggag ctactccttt gataaatcct
    12961 tctgcagcta attcatgtaa tctatctata gggaacatac agtttatatc tttgtttaca
    13021 tctccattgt catatccacc atgtgatacc atcatttcat caactgtagc tgtattaggt
    13081 atagctctaa atgtagtatc tcctgctaga ttaaatctct tgtcagattt taaatgaaca
    13141 ccagctgctg tagctaaagc tatagtcata tcttttaatt ctttagttac aggagtccaa
    13201 actggaggag gtgttattgg aacgaatatt tcagattgaa gtccttgtac tgttgtaagg
    13261 ctcatatatt tcctctccct tctattttca aacgattatt tattattttt cttcttgttt
    13321 tgattgcatt tctgcttgtt tttctttttc tctcctaata ttttctacat actcttgatt
    13381 aggctctgga cttaatacct caggatagct tagcatgaat ccaacttctt gacctatctt
    13441 aagttcgtag tcagttataa gcattctatt aggtatttta aattgtccaa gtctaccttt
    13501 taggtctata gtcactccac cacctttgat gtcaactata ataccttcat aaaaagtctc
    13561 tgatgagata tattttaacc tatccattat aattctacct ctctatactg ttttaaactt
    13621 ggatctaaat ttatgttatt gtctaaatct tagttagtat ctaaatttta gttttcgtct
    13681 ttttcgtata tttctcttct cttcttactc attggtagag attgctcatt ctcttctaat
    13741 actatttttt ctcctgtaac tttttctata gcttctatgt tatttaattt aacatttgga
    13801 ttccattttc tttcaggagc tttaacttct acaccttcta tagcattttt aagcatagcc
    13861 attattctaa cagcatcttc tggagctaaa gtgttgttag ataatatttc attctctata
    13921 ccttgcttag acttattgtt gtctaccatg tgagtcatgt atttattacc aacaactaga
    13981 gcaccttgaa cagctgagaa acttacacca actactggta tacctctcat acctatttgc
    14041 tctatgtgag atgcgaaatc tatatggttg tttccgaaac cttcagtagt tactacagct
    14101 ccatcaactt ccatagcttc aactaacata cctaatctct tagatacata gaatttctca
    14161 gcatttactt gtggagaacc aacaaataca acacctacta aatctatttc ttcgtcttcc
    14221 atagctctta ttactaatgg ctctctccag taatgtcttg acatttcttt tgatgcaggt
    14281 cctatacaag ttaatgcatg gataccacca tctaatactt caagtggaga taatacaact
    14341 ggcatgtttc ctaagtcaac gtttggttga gctcctaatg taccaactgg ctcaactggt
    14401 aatattaggt tatcatgcat tgcaccttgt cccattattt cttttataac aacaactttc
    14461 tttttacctt ctcttctgta ttgctctatt acttcttcat caactactaa agagttgtca
    14521 actttcttta atgcttctct tatttcttga gttacatagt caaatgcacg gtgagcagct
    14581 aaaggtcctg gtctttccat gttagttcct gcttttattg ttacttgacc tttgatgaat
    14641 atttcacctt tgtcagcagc acctggtcta ccccacatta tagtagtatt taactcacct
    14701 tctgaagaac caaattcacc tatttgaact ccatcttcat ctgtaccagt taataccatt
    14761 acagctccat ctataactct agttatacct gaacctaatt cgccttcttc tttagtagcg
    14821 attggttgta tatctaatac agcttcactg taagtttcat atttatcagg agttattatt
    14881 tctaacttca tatctacaac taactcttga gtttcattag cttctttaca tatttcttct
    14941 gcgtttctga tgtataaagt agttccttct attttagttt catcagcaaa ttcaacttta
    15001 tctattttaa agtgtttttt agttaagctt cttaaaagtt tcttttcttc aacttttgga
    15061 gctacttctg ctgctgcact tgccactgga gcagcaacgt ttgctactgg agcagcaact
    15121 cctgcttgac cagctactga taaaggtatc tctaagctta tgttcttacc ttcacctatg
    15181 tgtattttaa ctgtttcacc tgcaactcct gtgcttactg gaactactgc tgcttctgat
    15241 actggagctg cttcttctac tgtttcttct actacttctt ctttagcctc tcctgctttg
    15301 tatccttcta cgttatctgt agtcattgga gaaagtgcat ctaaagtttc ttttaactta
    15361 gctcctagta cttgacctat agttaatcca ttttctggta ttgttaataa tccagaatcc
    15421 tctaagtctg caaatattgc tggatcttct aagttttctg gcgctataat tgttcccgct
    15481 tcaaatctac aacaacaaac tgctgggtca tttgcatggg cttgagctgt ttctaaagtt
    15541 attgacatag tataaaatac ctccttacat tattttctta tttattattt taaatctcaa
    15601 ttcctcaaca ggtaacccct taactgagaa attaagtaaa ttaaaaccta cataatttgt
    15661 tatgattaat attatttttt tctcactcaa agtcacttta tattataagc aattcctatg
    15721 ccaacttatt aacaaaaaga ttttttctat aatagattct tcatctatcg ctatttttag
    15781 ccaaatatta tataaatatt tatttaaaat atgttatttt ttgtatttgt aaacattttc
    15841 tcaaacaaaa aaagtgtcaa ttaatcaaca ctttttcatt ttttttaaca catctatata
    15901 cacaacactt atacaagtgt tggctttact aggttttaaa ttaaagttta tcattttgtc
    15961 aaaatttagt catatgtcat attttagaca catctatttt gtattggtct atcttataat
    16021 acaaagttgt tctgggtata tttaatatct tagctgcttt agctttattt ccattagaca
    16081 ttttaagtgc cttggttata ttctttattt caattttttg agttgccttc gttaaatcta
    16141 agggatactc ttcttcttca gtactattat ttgtagaatc catcatataa cttggtacat
    16201 catctacatc tattttagaa ttttgtgaca atacaacaat attttcaatc gtatttttta
    16261 attctctaat attacctttc catctataat tctgaaatat atctattaca tctttgctaa
    16321 ttgtaagaac aggcttatta ttttgtttac atatctcctc taaaaagcta tgaaccaata
    16381 atcctatgtc ttcttttctc tctctaagtg gaggaatctt tatttcaaca acatttaatc
    16441 tatagtataa atcttctcta aatttttctg cctttaccat tttactcaaa tctttattgg
    16501 tagctgatat tattctagga tttattttta ttgtggtatc tccaccaact cttcttattt
    16561 ctttttcctg taatactcta agcaactttg cttgcatact aagaggtaaa tcggcaattt
    16621 catctaaaaa tacagttcca tcttttgcaa gttcaaatat acctattcga ccctttttac
    16681 ttgctcctgt aaatgcacct gattcatacc caaaaaactc actttcaaat aactcatttg
    16741 gtatggcact acagttaaca ggtataaatt gaccttttct ctcactataa tcatgaatag
    16801 ctcttgcaaa tacttcttta cccgtaccac tttcacccca aataaatatg ctactatttg
    16861 ttctagccac ttgttttgca atcgctttcg atttttccaa tttataactt ttacctaaaa
    16921 tcttatcaaa actaccttta gataaatttt tgacttcgtt ctttagatat tttaatgtat
    16981 catttacttt ttcaagttca agagctaata tcttagcttc tgtaacatca acttctgtac
    17041 atacaacacc gataaattct tctttataaa atataggatt tgcatgaact aatgcataca
    17101 tattttcttt tttatctgta aaatacaagt cactcattcc aactttacta tttagtactt
    17161 tttctgaaat agtattttcc aaaaattcat tcatcggtcg accaactata tcttcagact
    17221 ttatatcata cctttcctcc ataaacttat tccacaagat aacaacacct tcgttattta
    17281 tagcacaaat tccttcttta atttgaccta taatatactt caaagtcaat ccataatctt
    17341 ctaaatgcat atatagatag tccctaatgt gctcttgttt tagaactcca ataattttat
    17401 tatctctaag aacaggaaga atccctatat tttttaatat cattatatct ctacattcat
    17461 ctaaggttaa accttcgttg acatatatta catccttctt cattataagt tttactggtt
    17521 gaccttcata ctttttatgc atctcataaa gattatgtat atccgtcatg gatataatgc
    17581 cttttaattg gtcagatgaa tctattacca ttagagtttt tgtgttgctt tttatcattt
    17641 cttttattgc atcttcaatt cttgtgtctt cgtctatagt tgtaaatttt gtatccatta
    17701 cctcttctac tttttttact tctggtatcg aaaacataaa gtccccccct ttatagtagt
    17761 acctcatcaa aacttattta tatatattta t
//
LOCUS       DAMKPG010000077        17409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMKPG010000077 DAMKPG010000000
VERSION     DAMKPG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.081"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            52..798
                     /locus_tag="PC621_002722"
     CDS             52..798
                     /locus_tag="PC621_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF4109869.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            860..1534
                     /locus_tag="PC621_002723"
     CDS             860..1534
                     /locus_tag="PC621_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4109870.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            1902..3248
                     /locus_tag="PC621_002724"
     CDS             1902..3248
                     /locus_tag="PC621_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF4109871.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3275..4675
                     /locus_tag="PC621_002725"
     CDS             3275..4675
                     /locus_tag="PC621_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF4109872.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4885..6321
                     /locus_tag="PC621_002726"
     CDS             4885..6321
                     /locus_tag="PC621_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF4109873.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6665..7609
                     /locus_tag="PC621_002727"
     CDS             6665..7609
                     /locus_tag="PC621_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF4109874.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7778..8710
                     /locus_tag="PC621_002728"
     CDS             7778..8710
                     /locus_tag="PC621_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF4109875.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8815..9282
                     /locus_tag="PC621_002729"
     CDS             8815..9282
                     /locus_tag="PC621_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF4109876.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9526..10380)
                     /locus_tag="PC621_002730"
     CDS             complement(9526..10380)
                     /locus_tag="PC621_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF4109877.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10546..12510
                     /locus_tag="PC621_002731"
     CDS             10546..12510
                     /locus_tag="PC621_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4109878.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12510..12971
                     /locus_tag="PC621_002732"
     CDS             12510..12971
                     /locus_tag="PC621_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4109879.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12974..13294
                     /locus_tag="PC621_002733"
     CDS             12974..13294
                     /locus_tag="PC621_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF4109880.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13310..14410
                     /locus_tag="PC621_002734"
     CDS             13310..14410
                     /locus_tag="PC621_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF4109881.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14530..17130
                     /locus_tag="PC621_002735"
     CDS             14530..17130
                     /locus_tag="PC621_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF4109882.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6928 a         1787 c         3007 g         5687 t
ORIGIN      
        1 ttaatataat atatttatga aaaatgtaaa ttgtgaaatg gaggtagtaa catgaaaatt
       61 gaaaatcttt ctattgatga tattaaacga ggctatatat ttaatgatga tttaaattgt
      121 tatagttgca ttaaatgtac aaaaacatat gaggtaggag aaatatataa aataggtaat
      181 agattctttg aagcttcaaa agctattgaa cttcatatgg aattagaaca tagtgattat
      241 ttagagcaat tgcttaatag tgattcaaag tacaacacat tgactgaaaa tcaaaaacaa
      301 ttatttcaat tatttattag tgatttacca gataaggaga tagcaaaaca ggttggagta
      361 tccacatcta ctatacgcca tcaaaaattt attttcagag aaaaagcaaa acaggctaag
      421 ctctatttag cactatatga atctgtattt gaagataaat ctaataaaag tagtactata
      481 ataccaatcc atgaacatgc tcgaatggta gatgaaagat atgtagtaac tgaagaagag
      541 agaaaacaca ttttggatac atcatttgaa agtatgaatc ctttaaaatt aaaagtattt
      601 tcttcaaaag aaaagaaaaa ggtcgtaatt ttatctaaga ttttggaaca atttgaacgt
      661 gggaagaaat attcagaaaa ggaagttaat cagattttag aatctatata tgatgatttt
      721 gcaacaatta gaagatattt aattgagtat ggctttatga gtagaaataa agaatgtaca
      781 aagtattggt taacataatt aaaataataa attttactaa tgtgtaatgt tatttttatc
      841 taggggggag gagttaaata tgaatgatga attaaaatct attttataca ataaaggtgt
      901 agacattgtt cgctttgttg atatatctga atttccaata aatcaaacac aaggtttttc
      961 taaagcaatt ttattttgta tagggttatc aaaaaaattt attacagata tatacaataa
     1021 cttacctaca gatagtgatg agtttttgga aaaagaggag aaagtagaag aattagctga
     1081 ctggatttca aaatatattc aaaataaagg atatagagcg tattctcaat cggagaaaaa
     1141 caatctagaa catggatatt ttgaaaaagc ctatattaat ccagaaatgc aatcagggat
     1201 tagtccttta ccacataaga caatagcaaa tattagtggc attggattta tgggaaaaaa
     1261 taatttgttt gtaacagagg aatacggatg tgcttttagc atgtgtacag tacttacaga
     1321 tgctccaatt tctgttgaaa gatatccttt gattgattca aaatgtttaa attgtaatgt
     1381 ttgtgtagag aactgtcctg caaaagctat acatggaaat gaatggactt tacctggaaa
     1441 aagagaatct attattgatg tttcaaagtg tttttgtgca ttgaggtgta tgatgagttg
     1501 cccatggtca ttaagatatg caaatcaaaa ataactttac tagagaaggt attaaattga
     1561 atattataaa atttttaaaa tgtaagatta tataagattt ctcaaaataa aaatttgtat
     1621 agaacactca gaaatgagtg tttttctatt gggattaaat tatattgata caaagcttaa
     1681 ctattttaat cattatataa aatttgtatt aaaaaattaa gaataacttg ctaatagtaa
     1741 aaatataatg agattataat tgatttataa tataactgta atgatgttgt aaaatgaaaa
     1801 ttgtcatctc aaaattccca aaaagagtaa aagtcacttc ggttatagtc aaaaaatttt
     1861 ttctgtgaaa agaatttaga tttaaaaggg aggaattaat catggaaatt gaaactaaaa
     1921 ctccagattg gattaatgta ctagaattaa acagtaaaat agatattact tggaaatatg
     1981 atgtaagtaa tatgatacaa gatattgtct ctgataagtc actagaaggt tccattatat
     2041 attttccaaa aggtaattac ttatttgaga aaggtattaa aattagtcaa caaataacac
     2101 ttcaaggaga ttcttattat ggaggagata atcaagtatc aaatttagat aaaaaacaat
     2161 caatcgtagg aacaaccaat tttataaccc gaggagtttc taatataagt attattacat
     2221 tggaaggaac aagtcaatgt atcaaaaata ttaattttta ttatgatagt catgatatag
     2281 agaaaatacc acctaaaaat gtatctgcta ttacagaata tggagaaaca caaggactat
     2341 ttcactttga acacctgttt atttctggat tttcaggaat agggattgaa attccatact
     2401 attcaactgg caatgatatc attgttagtt catgtggtct tggtatgagg cttggagaga
     2461 agtcaatgct ttctagtagc aagatttatg aatgtaaaaa tggaatggag attactactg
     2521 gtgttagctt aaataatgtg agaatagaag aaataagaga aataggtatt aataataaag
     2581 gatttggttt taacctgatt atgaatctta tggtcgacca atgtggatat tgtggattta
     2641 tgtttgaaaa aatgtgttat agccagataa ctgccagaat tactaaatgc ggtcagtatt
     2701 ataaaagtgt tgattataat acttatgaga atatggaaga tagaaaagaa gaagcttatt
     2761 ccatttttta tggagatatt ttagaaaatt gcaatattgt attgatgaat agtaatattg
     2821 ataccttaga aaatgattca cttgatgaac ataagatata tgtaatcaag gcaaatgaaa
     2881 caaacaaagt aatgttaaca tgttatgctg aagcagatga gcttatacag tgcgcaaagg
     2941 gcaacttgtt gctagaaaat ggtgaaaata cttataaatt ttataatggt gagatttgtt
     3001 ctgtaggagg tgttcaaatt tctgatatag atgaaaaaga tttaatcaaa ataatcgaga
     3061 acactctgta tatagatgat aaaggtaagt acttaatggt tcctagatta aaaaagaata
     3121 ctgtaatata ttcagtacta gattctatag aagaaataaa taaattacat ggtggaatat
     3181 gggaacagat tgatgtaaaa gtattagctg gggaatctat gtactattat aaaaagataa
     3241 aagattaaat tatcaaacta ggaggtagtg attaatggaa gagtctataa taattaaaga
     3301 tataagtggc tggattaatg tacttgatta tgggatttgt aatgatgaaa caaaagatgt
     3361 aagttccaag ttgcaagaga ttatagatgc tgcaccagaa gattctatta tatattttcc
     3421 aaaaggaaaa tacttatttg aaagtggtgt taagattaag aaacgattaa ccttatgtgg
     3481 agattcttat attttggcga atgttcctaa aaacactgct ggagtaacac aatttacttt
     3541 taaaggaaag aaaaatccta aaagtgaaaa tatagcaatt ataacagcaa aaagtgtaag
     3601 acactgtatt aagagcattt gcttttattc agatagttgt gatagagata tgacatctaa
     3661 tcatgaacct ccaacagatg gaaaacctag atttcatcat aatgtaatta taaattatag
     3721 aaatatatcg gctattgtat gtgaagataa acagggagaa ccaggtcatt atgaaaactt
     3781 gttttttagt ggtttttctg gaattgcact tgatatgcct aataattcta cagcaaatga
     3841 tattacagtt tttacatccg gacttggaat tagtacaggg gaaaattcaa cgattgctaa
     3901 cagtaaggtt tggggctgtg ttgatggaat gataatttct actggaactt ttataaatgg
     3961 tatgagggta gaagaaataa aaaaagttgg aattaaaaat attggtagag gtttaaattt
     4021 tattacaaat atcacaattg accaatgtgg atattgtgga gtttggtttg attctatatc
     4081 caatgttcag atatcaggaa atatcacaag gtgcggacag tactattatg atgtaagcta
     4141 tgataattat ttaatgttgg ataatagagt taaagaagca tattctttat tctatggtaa
     4201 tttattggag tcaagtaata tagttttgtc aaataccaat aaagataatt gggaagattt
     4261 caaagaggat aagcataagg tgtatcttgt agaagcactt gaaaccaaga atgtactatt
     4321 aacatgcaat atagatgcta ctgactggat tgttaagtca gaaaaaggaa atttaacgta
     4381 caataatatg cgacaaactg ttatatttta cgatggaaaa ctaagtagta tcaatggaat
     4441 tggtatatct gatgaagatt atggggataa agtcaagata aaaaaaggaa atatatatat
     4501 aaatagtaag gataaggtta ttaagatgca taaaccagag gtaggtgaca ttatgtcaac
     4561 aattattaaa gatgaaaata tactttccgc tcattatggg ggaacatggg ggaaaattgg
     4621 tgaaaaaata gagtttggaa taactgtaca ctattataaa ttgatagaga aataagtaaa
     4681 tacgtagtat ttgaatagat gtattttatt ggagagctag gactatagtt ttaatgtaaa
     4741 tggtttggta atagcatgga ttgaataata taaaaataat ataaaaaatt aatttgatta
     4801 tgtaaaattc tatgaagtag taaacctgtg actttagtca tggtaggttc aaattaagta
     4861 aatgggaaag gaatatataa tattatggaa ggaagaactg ggattaaaga tggaagatgg
     4921 attaatgtag tagaaagagg tattaaaaat gatggttcgt acaacatagg tcctgattta
     4981 caggatatta ttgatgaaat agaaacagat ttctgtgtct tatattttcc aaaaggaaaa
     5041 tacttattta caagtggtgt taaaattaat aaagaaattg tattacaagg tgattctaat
     5101 tcacctacag agactacaca atttataact cgtggagtag aaaatatgag tattattaca
     5161 ttgacaggga agaaacaatg tattaaaaat ataaatttct attctgatag ttgtgagata
     5221 caggtaaatg aagaaccacc gacaaaaggt aatcctaaat atcattatga gatgattatt
     5281 aataagaatg aaaaaaatgt tgagatgaat aatgtatcag caattctgta tgataatagt
     5341 aagaatatga ttaaaggtct aggtcattat gaaaatattt atataagtgg tttttcgggt
     5401 acaggaatta ggataccata ttactcaatt gtaaatgata ttacagtttc atcttgtggg
     5461 ttgggtattg atacaggaat agacacaatt atttctaata gtagaatatc taagtgtcaa
     5521 aatggaatga ggattagaac tggaactagc ataaataatg taagaattga aaaaatacag
     5581 aaagttggtc ttataagtag tcaagttaag gattatgagg gatatggaag ttataaaatt
     5641 aataatatta cgattgacca atgtgggtat tgtggttttt tatttgactc agtgaaaggt
     5701 agttattttt ctggtattat aagaagatgt gggcagtatt attataatac agattatgac
     5761 acatatttaa gtattaaaga tagagtagaa gaagcatatt ctttatttta tggaaattat
     5821 tttacagaat gtaatattga tttagttagt gataataagg ataactggga tgatggctta
     5881 gaatataaac ataaagttta tgtatttaaa acccttcaaa taccaaaatt aacattgaga
     5941 tgcaatatag atgcctatga ttatatcgtt aaatcggtac atggaggagg taatttaatg
     6001 ttaaaaaata actttaacaa ttatatattt attgattcat ctagtccaga agttattaat
     6061 ggaattaatt ttaattatca aaagaatgga aacctgttaa ggttaaataa tggtaaatta
     6121 agttataaaa atggaaagga agaaacagct atattcagac ctaattatgg tcttattgta
     6181 gcatcaacag attcagaatg tgaacaaata gagactagct atggaggaaa atgggagaag
     6241 cttggttctg aagtaattgg aaaagaaacg gtatattatt ataaacatca tgaaccagat
     6301 aaaacaaacc aaataaaata aaatttgtat tatataatga tataaagttt taatatatag
     6361 tgtttacctt taatgtaata ttggtatatt tgtatagtat taaatttgtt aaaaagtaaa
     6421 attgtagggt gcttgaaaat agcacctttt ttagtttcat tataaaatta gttttgaaag
     6481 tttaggaata atgtatattt atttttaaag aattataacc ttattaagga tattttaaat
     6541 gctagaaatg aatcagtaaa tcatactgga attatcttaa atgttttgat taaattaagt
     6601 atttagagct ataattatat taaaaatata ttaggacata attatattac tatggaggtt
     6661 aaatatgata gtaagtgtga gtagaagaac tgatatacct gctttctata gtaagtggtt
     6721 ttttaacaga cttaaagaag gttttgttta tgtaattaat ccaatgaatc caaaacaggt
     6781 tagtaagata gagttaaatc cacatactgt tgattgtttt gtattttgga caaaggatgt
     6841 aactccaatg ttatatgatt taaataaatt aaaagactat aaatattatt ttcactatac
     6901 tataacatca tatggaaaag aagtagagac atgtatttta gataaaagaa aggtaataga
     6961 ttcatttaag gaattgtcta gaaaaatagg caaagaaaga gtaattttaa gatacgaccc
     7021 gattttctta agtgaaaagt acacagtaga ttatcatatc aaagcatttg cgagtttatg
     7081 taatcagctt gatggattta cagaaaaatg tataataagt tttatcgatt tatataagaa
     7141 aacaaaattt aatactaaag cattacatat aaagccaata gaaattacag aaatagaaac
     7201 attatcaaaa gaaatttcta agattgctca taagcatggg ataatacttg aaatgtgttc
     7261 tgaagaatat aatttaagta aatttgggat tggaaaaagt aagtgtattg atgatagatt
     7321 aatatcaaaa attataggat gtgaagtaga ggtaaaaaaa gatagtacac aaagagatat
     7381 ttgtggatgt gttaaaagtg tcgatatagg acaatacaat acttgcagac attattgtct
     7441 gtattgttat gctaacttta attatgcaaa agtagaagaa aactgtaggc tttataaaga
     7501 agataataaa ttactcatag atactttaag agatgatgca aaaataagca ttagagatat
     7561 gaagactatt aaagtagata agaataaaca aattagtata tttgattaaa ataaatatac
     7621 taattacctt aatatatcaa gaatatagat acttttgata acaaaattaa acttaataga
     7681 tattttctat ataagaaaga tggtcataat atgtcaattt gaccatcttt ttgatataat
     7741 taaaacacaa ataatttagt aacaaaggag gcagaacttg aatttattaa aaaaatatcc
     7801 tatacctatt gcaagtttga ttttgtcaat ttttacttta ggtaacttat tacaatctta
     7861 tagtgaatca ttacgtaatg taattggatt tataggattt attttgtatg caatttatat
     7921 agtcaaaata atattactta gaaagggtgt taaagagcaa ttagagaacc ctttaatatc
     7981 aagtacattt ccaactataa caatggcaac aatgatattt gctacatata taaaaccatt
     8041 atctttagaa ttaagtatta tgatttggag cataggaatt gtgggtcatc ttgtgttaat
     8101 agttttattt agtaagaagt tcttagttaa attctcaata aaaacggtat ttccatcttg
     8161 gtatatagta tatgttggga tagttgcagc tagtgttacg gcaccagcat ttaatcaact
     8221 tttgattggc aaaattgctt tttggatagg atttgtatct tatatagttc ttataccagt
     8281 aattttaaag agagtatgga taatcaagga aatgcctgaa ccatctttac caagtatagc
     8341 tatattagct gcaccagggt cactattatt gtctggatat attaattctt tttcagataa
     8401 aaataatgta gtattatact tgttattcat attatcaatt gtattttata ttatagtttt
     8461 gggatactta ccgaaattat taagattgcc tttttatccg agttttgctg catttacatt
     8521 ccctatggca ataagtgcaa tgggtatgaa actaatgaat ggttatttaa ctaaatcagg
     8581 tcattttata agttggattt catatgcttc taagttagaa gagataattg ctacagtgat
     8641 tattttatat gttttagttt tatttattat atatcttagt aaatctgaaa ctacatctaa
     8701 agctaaataa gaattattaa atatgtgtat ttaataggaa tattgatata aatatatgaa
     8761 gataagataa accatagaaa cttaatttag cttaatttta ttggaggaaa acaaatgaaa
     8821 acaaaagaag aatatttaaa agaagttaga aacatatcag aagaatactt tagaaaagga
     8881 gagttttttt gctctgaagc agttcttcag acaataaatg atgctttagg ccaaccttta
     8941 agtccagaga taactaaatt agcatcagga tttccgatag gacttggaaa agctcaatgc
     9001 ctttgtggag cgatatctgg aggagagatg gctttaggta tagtatatgg aagagtacat
     9061 ggagaaacta tgaatccaaa gatgtttgag catgctaaag gtttgcatga ttatattaaa
     9121 aaagagtatg gtgcaacatg ttgtagagct attacaaaga aatgggatgg tgataacttt
     9181 atgtctcccg aaagaaaaga gcactgtatt aaaataacag gtcaagttac agagtgggtt
     9241 gcagataagc ttatagaaga tgaaaaactt aatatcaaat agtgtaatat tagttggtga
     9301 atttataaag ttaaatttac ttatagtata agatacttga tattctcgta ttgatagaaa
     9361 aatttatagc atatgtattt aatgaaatat acttatattt agagtgtggt ttaatttcat
     9421 aaatacatat gctattattt taagtatata agcaaattta tttaaataaa tagaaacagt
     9481 taatttatat gtacaaaact atatatgatt agtgtaaata aatacctatt ttataatact
     9541 caacataaag acaccagaca tgattagtat tataccaata aaaatagcaa gccattcttt
     9601 tttgttcttc ttttctttaa aaaatataat ccctcctaaa gtggctacta tcacacaact
     9661 ctgagagata gaaaaactta ttgtaactcc caatgtatta gtcgttatga acatacctaa
     9721 atttgctatt gaccacaata taccagtaat taaattgaat aaaacatttt ccctatatac
     9781 aatctcttta gaaaaatact tgacaataat agatgaagtt atcaacatac caacagattg
     9841 aggtaatata attctatatc cttcaattaa aaatatttga ttagttacaa catataatga
     9901 taaaaataga gatgataata atacaactcc aacacatttt aacatatttt tatctgtaga
     9961 ttttttagtt tgataattag tgagaagtat accaagtatt atacataata ttgataaaga
    10021 accaatcagt aacattttag aactggtcca ttcattaaac aagattacag ctaataatgt
    10081 cgtaaaagtt aattgtgaac catttgaaat tggcattact ttagatacac tagcaatttg
    10141 aattgcttta aactgcatta attgtcccaa actccagaat atgccagaaa tcatacttat
    10201 acaaaatgaa aaaaatgtaa tttcaggatg tataaccaga gttattatag tggaaaatat
    10261 aaacgcactt atggatgtac ctaaaagctg ttcatgagga gaacttttct ttagatttgc
    10321 aactatgggc atcaatcccc atcccattac aggaagtaaa gcaataatta ttgtcagcat
    10381 taaactattt cctttcatat taaatttaga cagcttttaa tttatcatct aaaattaaag
    10441 tgttcaatac ataaaaatta ccgtatttaa tcaggaaata ttagtagttt tttagaaaaa
    10501 cattttccat taaaataaag ataaagggaa ggagtaaaat tgaaaatgtt aactgatatg
    10561 attcaaaatc gtgaaattaa aataataaag tttttattaa aagaaggtca tacaactgta
    10621 aaagcaatag cagatgattt agatgtttca gaaaaaacag tttcaaaatc tcttaaagaa
    10681 gtagaagcag tgcttaaaaa gataggtctt tctttaataa gaaaacctaa gataggtgtt
    10741 tatattgatg gagatttaaa aaaaatactt ccttacctag ataataaagg aagtcaaatt
    10801 cctacaacca gagaggagag agttatctat atatattcag tacttttaaa gagtaatgat
    10861 tatattacta tacaacaact ttctgatgaa ctatatataa gtcgtggaac tattgaaagg
    10921 gatttatcag aagttgaaaa actgctaaaa aacgaaggga tttccatata taagaaacct
    10981 agtaagggaa taaagttaaa tataagtgaa agagataaaa gacaacttac tgctaagttt
    11041 atacataaat tttggagtag aaactggtat attaagcaag agcaagaaag cttttatcaa
    11101 gcatttgaaa agatacaggc agatgtaaat ggtatttttt cggaagatga tttatcaata
    11161 attattgata ttcttaggaa tttcagcaga gaaagaaaat ttgagttctc tgattatgaa
    11221 tttcagtcat tagttattca tttggcaata gctgtagaga gaattagaaa aggcgaatat
    11281 atagaagaaa gtttgtatat tggtaatata gaaaataatc aaatgtcaaa taccaattat
    11341 ttagcaaaac aattagaaga aaaattttca atattattac caaaatctga aattggatat
    11401 ataaatttac atttagtagc ggctgaccaa catagagata ctaatgaact aaaaaatact
    11461 tcaactaatc cttgtgaaac aagtacaaat aatatatcaa atcttcggga aattataaaa
    11521 aatacattgt atgagacagc ttatgacaag gatttaattg atggattgac ttcacatgta
    11581 cattcatcaa taaatagact gaaatatgga cttagtatta aaaacccata tgtaaataca
    11641 atcaaacaaa acttttcttt atcatttgag caagctttag atttaaagaa agtaattgaa
    11701 tttatatatg atattagaat aaatgatgat gaatgtgctt atatagcatt acattttgaa
    11761 gcttttagag aaagaatgaa agaacttcca caaaatatta gagtaattct agtatgcagt
    11821 acaggattag gttcatctag gttattgtct gcaagaataa aaaaatactt tccaatgata
    11881 aagattgaaa aagtactttc cgtacaagaa ttagtatcca gtaaaattga tgcagattta
    11941 gtaataagta ctatttattt agagttagaa acaattccaa ccatagtagt tagccctatt
    12001 ttaaatgaaa gtgatataag aaccttggaa agaaatatac aaaatgtttc atgttgtact
    12061 tctaagaagt ataaatcttt cagaaaccta atctttgaag aaaatatatt gttaaatatt
    12121 gaagcttcaa actatgaaga tgcaattaag tatataacag ataacctaat aaaaaaaggt
    12181 tttgtaaaag aaggtatagc taaaagtgca attgagagag aaaatttatc ttacacgtct
    12241 tttcagtcaa tagctacacc tcatgctaat cctagttata ttaaaaaatc taatatatct
    12301 gttgcaacgt taaaaaatcc tattacatgg ggaaatgaaa aggtggaagt tattttcttt
    12361 atttcattag aaaatgacaa aaacctagag cttgatgaaa tatatgaagt tttttatgac
    12421 tttattgaca ataaaaaaaa tataaatgac atattaagtg caacttccac aaaggaagtg
    12481 tacgatttgc tattggagga gagtatatag tgaaaatatc agatgtattg aaaaaagagc
    12541 aaattgtttt taatcttgat acaaatacaa aggaagaaac aataaaaaaa ttaagtagta
    12601 tatttgttga aaatgggatt gtggacaatg aagaagaata tgttaagtct gttttggaaa
    12661 gagaagaaca ttcaactaca ggtattggta atggtattgc tattcctcat ggaaaaagtg
    12721 atgctgtcaa aaaatcttct ataatatttg caaagactaa gcataagatt gagtgggatg
    12781 ctttagatga aaaaccagta gatagtattt tcttattggc tataagcaat attgataagg
    12841 atagcaatca tcttgtacta ttatctaaat tagcaactaa acttatggat gatgacaatg
    12901 tagatgcact aaaattagcc aattcagaac aagaaataat caatatattt agtgaagaag
    12961 gagagatgta gaaatgaaaa gaaaaattat agcagttaca gcatgtgcaa caggagttgc
    13021 acatacatat atggcagctc aagcgttaaa gaaagcttca aaaaagatgg gtcaccttat
    13081 aaaagttgaa acacaaggag ctacaggaat cgataatgag ttaactacaa aggatgcaca
    13141 aattgcagaa gtagtaatat ttgcagtaga tactaaagtc agaaatgctg aacgttttga
    13201 gggaaaagaa atactagaag taccagttaa tgcacctata aaggatgcag agaaagtaat
    13261 aaatgatgca ttaaagttaa ttgacactaa ataaatggag ggaaatgtaa tgttgaaaaa
    13321 gatagtctct gagttaaaaa agcatgtctt aactggaatc tcatatatga ttccattagt
    13381 aattgcaggt gctatgataa tggcaatatc tcgtgtaggt ggttctttct ataatatacc
    13441 agatatatgg gatgctaaat acgcagaaag tgctagtagt attgttagat tattacatga
    13501 tctagatgga tttggaggta ctgcattagg attaatgttt cctgtaattg ctgcatttat
    13561 aggattttct atagctgata aattagctat tgtaccaggt cttgttggag gtatgattgc
    13621 taaagatata ggtgcaggat ttttaggagc tttggcagtt ggtcttatag caggatatac
    13681 atgtttgttt attaaaaatc atgttaaatt accaaaatca gcagcttcaa tagtaccaat
    13741 atttatagta ccagtatttg gaacattaat cactgttgta ttaatcaatt atgtgatagg
    13801 tatacctttt gccacattaa atactggatt agaaaattgg ttaaatggat tatctggtac
    13861 aaatcagata ttaatggcag ctattatagg ggctatgatg gcagttgact taggtggacc
    13921 agttaataaa gcagctttaa caacatcact tgcattatta acaagtggaa tttatgcacc
    13981 taatactgct gcaatggtag gaatagttat accaccacta ggattgggac ttgctactat
    14041 actagcaaaa caaaaatata ataaacagtt acgtgaagct gggaaatcat ctcttataat
    14101 gggtttaatt ggagtaagtg aaggagcaat tccttttgcg gtagaatctc cattaaaagt
    14161 aataccttca tgtgtaattg gaacagcaat tgcttcagct atggcagttg gtttaggttc
    14221 agtaaatgca actccaatta gtggatttta tggatggttt acagtagaga attggccaat
    14281 gtatgtgtta tcaatagcag ttggaacagt aattgtagct ggattagtta tagtattgcg
    14341 tggaaatcca gtgattgagg atgatgaatt tgaagatgat gattttgatg aagcagagtg
    14401 ggaatcttaa aatttaataa ctatttaaga cgtgttataa gctatattca atatgaatct
    14461 tatactaaat aaagtgttaa atattcagtt tagacttaaa tataaaagat aaagaacaga
    14521 ggagaataaa tgataaaagc acatattgtt aatcatacac actgggatag agaatggtat
    14581 tttacttctg gagatgcatt agtacttagt gaacaactat ttacagatgt aattgatgaa
    14641 ttagaacgta atccagatgt aagttttgta cttgatggac aactttctat tttggatgat
    14701 tatgtacaat tacacaaaga aaaaatagaa atcattaaaa agttaataaa agaagataga
    14761 ctacatattg gaccatggtt tactcaaact gatgcattct ttgctagagg agagtctatt
    14821 ttaagaaact taatgattgg aatttttgaa agtaaaaagt atggtaagta tatgaaaatt
    14881 gggtatttgc cagatacatt tggatttaat gcacagatgc ctatactatt aaagcatgta
    14941 ggtcttgata atatattctt ttggagaggt atacatcttg gtaaacatgt tcaatcacca
    15001 tattttaagt ggaaaagctt aaatggagaa agttatatat atgcagtaaa tatgccacat
    15061 ggatatggaa ctggtatgct tttagagcct tcattaaagt atgttaatgg acgcttagac
    15121 ccagctattg attttattaa atcatatact gatgtagatg aggtgttaat accttctggg
    15181 aatgaccagt taaatataat aggtgatttc aaaaataaaa ttaaagcgat taatgatatc
    15241 ggaaagtatt catatataac gagttcatat caagagtttt taaaatatat aaagtccata
    15301 gaatcactag aaagctatag aggtgaattt agagaacctg tattagcaag aatacataaa
    15361 tcaattggtt ctagtagaat ggatataaaa ttagcatgtg ataaactaga aaacaagctg
    15421 attaaaagaa tagagccatt attagtaatt gctaaaaaga gtaaaattga aataagcaat
    15481 cagttactta taaatacatg gaaaaaactt ttggaaggac aagcacacga tagtctagct
    15541 ggatgtgtaa ctgatactgt aacagattat attcttcacc gtataagaga agcaaatgaa
    15601 atatgtgaca gtattgaaaa tactattgta aaaaagattt cagaagggct taaattatct
    15661 aaaaatgata ttttagtatt taatacagaa gctaagcgtt ttaatggata taaagagata
    15721 caagttgtaa gtgatagtaa aaatatttat tttaaagata atttagatgc tactataatt
    15781 gaagaaactt atgtaaaacc gagagaaaat gtattagaag aaacacctgc tggcaacgta
    15841 tttattgaag aatcaggata ttatatatta aaagtaagaa taaatgtaca gcttcctgca
    15901 cttggttata aagttgttag ttttgaatca agtgataaag aaatggtatc acttgataaa
    15961 tctaatgaca cttttatatc taatgataat tataagataa tctatgaaga tggaagctta
    16021 aatttacaac ttaaagatgg aacttacata tcaaactttt taatattaaa agatagtggt
    16081 aatgcaggcg atacctatga cttttctcca ttaaaaaatg acgaggatat aaaactttca
    16141 tttgatagag tgtatacaga aaaaacttta ggatatgaaa agttggtggt taaaggtact
    16201 actttactac cacttacatt agaagataga aaaaacaaat atcttaatgg gaaattaaat
    16261 gtaaaagtaa gtattgtctt atccaaagaa aatccattga tggatgtaaa actttgtgtt
    16321 gataacacga tatataatca tagactacgt gttcatataa aaactgatat aaaagataat
    16381 aaaaacatag catcattgcc atttggatat ataacaagag aaaatggagt tttagacaac
    16441 tgggaagata tttacagtga aatgcctata gatttagaac ctttggaaag taatattact
    16501 ctaacaaacc aaaatagaag ctgtactgta tttactagag gaataaaaga gtatcaacat
    16561 attgaagatg aaattgcatt gaccttactt gctactacag atgaactagg aaagcctgat
    16621 ttattatata gaccaggacg tgcttctggt gatacaacta aaaaaggtca tataagaatt
    16681 aaaacagata aagctcaaat gcttaagaaa ttagaatttt catttgcaat atatatggat
    16741 tctaaatcat ttgatgaatt ggaaattgcg aatttaaccc acaattatct aagggaatgt
    16801 gtaaattatc aattacagga ttacaatttc tttttatatc gtatagataa taagattcaa
    16861 aaatcaattg tcaataaaac tgtaagtaga gaattagaaa taatatcttt accagaaaaa
    16921 tatcttatat cagcatgtta tccttcttat tacagtaaag ataaatttat aataagagtg
    16981 gaaaatccaa gtagtaatag aatgatattg gatgagcaaa tatttaaaaa tagaaatggg
    17041 aaaattgtaa atgcaataga agatgtagaa gaagaacagg tatatgaaat agcacctttt
    17101 gacgttattt caatattatt ggatttataa gtgtaaaagt aagtttattg tttaaagtct
    17161 gacagatatt aatctgccag actttttaat ttgtataaat ttgggtaaat attaatgaat
    17221 agttttagta tttaaatatg aatttagtat ttaatataga atttattgaa gtagaataat
    17281 tagtttaaca ggataagcag tttaaataaa gaattattat attagagtga ttttatatat
    17341 aatttaaaat aataaaatgt taataaaatg agttaaagat tgtatattaa tagggttgat
    17401 gtagttaca
//
LOCUS       DAMKPG010000078        17065 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMKPG010000078 DAMKPG010000000
VERSION     DAMKPG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.082"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(98..784)
                     /locus_tag="PC621_002736"
     CDS             complement(98..784)
                     /locus_tag="PC621_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF4109883.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(833..1366)
                     /locus_tag="PC621_002737"
     CDS             complement(833..1366)
                     /locus_tag="PC621_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF4109884.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1410..2042)
                     /locus_tag="PC621_002738"
     CDS             complement(1410..2042)
                     /locus_tag="PC621_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF4109885.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2132..3211)
                     /locus_tag="PC621_002739"
     CDS             complement(2132..3211)
                     /locus_tag="PC621_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF4109886.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3344..4690)
                     /gene="pepV"
                     /locus_tag="PC621_002740"
     CDS             complement(3344..4690)
                     /gene="pepV"
                     /locus_tag="PC621_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF4109887.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4759..6039)
                     /locus_tag="PC621_002741"
     CDS             complement(4759..6039)
                     /locus_tag="PC621_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF4109888.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6166..6426)
                     /gene="hfq"
                     /locus_tag="PC621_002742"
     CDS             complement(6166..6426)
                     /gene="hfq"
                     /locus_tag="PC621_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF4109889.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6540..7475)
                     /gene="miaA"
                     /locus_tag="PC621_002743"
     CDS             complement(6540..7475)
                     /gene="miaA"
                     /locus_tag="PC621_002743"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF4109890.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7513..9480)
                     /gene="mutL"
                     /locus_tag="PC621_002744"
     CDS             complement(7513..9480)
                     /gene="mutL"
                     /locus_tag="PC621_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF4109891.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9491..12334)
                     /gene="mutS"
                     /locus_tag="PC621_002745"
     CDS             complement(9491..12334)
                     /gene="mutS"
                     /locus_tag="PC621_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF4109892.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12497..13231)
                     /locus_tag="PC621_002746"
     CDS             complement(12497..13231)
                     /locus_tag="PC621_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109893.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13200..14213)
                     /locus_tag="PC621_002747"
     CDS             complement(13200..14213)
                     /locus_tag="PC621_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4109894.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14235..15005)
                     /locus_tag="PC621_002748"
     CDS             complement(14235..15005)
                     /locus_tag="PC621_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109895.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14980..15270)
                     /locus_tag="PC621_002749"
     CDS             complement(14980..15270)
                     /locus_tag="PC621_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF4109896.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15401..15500)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16120..16209
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16262..16438
                     /locus_tag="PC621_002750"
     CDS             16262..16438
                     /locus_tag="PC621_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109897.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16553..16909)
                     /locus_tag="PC621_002751"
     CDS             complement(16553..16909)
                     /locus_tag="PC621_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109898.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         5384 a         2928 c         1800 g         6953 t
ORIGIN      
        1 tttgatgata aaagctattt tccttctaaa taaaaaattt gtctaaagtt tctaaaaaat
       61 ttagacaaat cttaggttat tattctttaa attattttta aattattcca aatacaccaa
      121 ataatattgg tataaatata gctggtaaca aattagctac ttttatattc gttaatttaa
      181 gtacatttag accaagtcct ataattaata aacttccagc tgctgtcatt tctgtgacta
      241 caggatcact taaaaatcca tttaaaaatg atgctcccac acatataata ccttcataca
      301 aaaatacaga tattgcagaa aacattacac ctatacctaa agaagcagta aatataattg
      361 aagctattcc atctataaca gattttacaa ataaagtatc atgacttcct gtaagaccac
      421 tttctaatga accaactact gccatggctc ctatacagaa taataaacta gatgatataa
      481 aaccctctgc tatagaatct tttttatttc ctttagaaaa tttactttgt aaaaaagcac
      541 ccaacttatt tacccatttg tcaatatcta ttaattctcc tatcagagca cctacagcta
      601 cggatattat tgtcactaat gtgttttttc cttctagtgc tcctgatata ccaatgtaca
      661 atacacataa ggcaattcca ttcattatag tatcacttac tctttgaggt aatcctcctt
      721 ttattataag acctactata catccacctg caattgctag actatttgct attactccaa
      781 acattttttc tcccctcgat tagctaattg aaatttatcc tattattaaa acctaagcat
      841 ctatactttg tattggatat tttaaatatt ccaaaaattc ttcaaaacct tttctagata
      901 tatttacagt tttagtattt atgttaggat gaaaatttaa gtaatcttct ttaattacat
      961 ctttatctat gtaaagctca acttttcttt ctgtatcatt tattaatgaa aaaggattta
     1021 cacttccagg aagaagttta agtacattgt atagtctttc aggtgaagca aacgataatc
     1081 tagaacttcc tatcttatct ttaagtgagt ttaaatcaac ttgtttttct cctttaacta
     1141 caataagata atacttattt cctttggcat ttcttaaaaa taaattttta cattgacacc
     1201 cctttgttat tccatctaaa atgactaatt gctctgctgt atatacagct tcatgctcta
     1261 ctacatcata ttcaacattt aatttatcca aaatatcata tattttagtt tcattcatgc
     1321 tagattcatt ttcatcttgt tcttttttat tatttaaatc actcatactt ttctccttaa
     1381 acacttcatc ataatatcat tgtgaggtat tattctacta tagctattgc tcttactgga
     1441 gctccatcag catcattata cttaaatggt gctgctataa atagaaattt ttctggaaca
     1501 ttttctaaat ttgtaagatt ttctatgatt atttttcctt tttcaaataa aatatgatgc
     1561 acttcaaata ctgatgtgtt atatctatcc actgatagca tatctattcc tatgcctttt
     1621 atattagtat tagctatata gtttgcagct tcttttgtta atgtaggata acctacatag
     1681 tactgctttt tatcccaaaa ttttgaccaa cctgatttaa atattataaa atcactattt
     1741 tttatttcat cttcatattt ttttagatat tctaattcta tattctcatt tcccttttca
     1801 agttccaata cataagcttt cccaacaaaa ttttctatat ccaacttatc tagggtctca
     1861 cctttagtat acatatgtct tggtgaatcc atatgagttc cattgtgaga aaatacacta
     1921 attaatgttt cttgataacc attttcttca ataatagcta ctttcttgat atctggtcta
     1981 ttaggctctg catatacagg catgtcatta tgtgtcacat gagttaaatc aaaaaccttc
     2041 atctttatat accccctatg tttttatttt ctattatata cctaaattca ttttatttct
     2101 atcagatttt attccattat ataccatact actatatatt ttttataaac ctcaagggat
     2161 ttaccgaaaa atcttggaaa gaattatcag taaatataac atcttcaccc aaaaaacgtt
     2221 tataccttcc ttcttctttc tttaaagtaa ttctctgttt ttgtgaatga ttattaaaaa
     2281 ccattatctt aagaggaggt ttttttctat tagtctgcac tctttttaac atgtatttta
     2341 tgtgcatatc agcttcagga aatgaatatc cacaaaatat taaaaggtca gtatctctta
     2401 aagttttttc tgtttcattc caaacattac ttaaaaatat gtttgacata ttcttaaaat
     2461 atgttggtgg cactataata ggttctgtta actcaccaca ctctaaacat tttgtctctg
     2521 aactattttc aataagcttc atgacaccac cttcatgtgg tgtaagagtt acactattac
     2581 aaatggggca ataaagccaa ttcaatgacc catgtatctt atataatttg attaatggtt
     2641 cttttggttt tctccaattt aactcctctt taaaatttgt aaactccaca ccataatcaa
     2701 gcataattgg caaatcattc ttatctctta atccagtaac tgtattgtct atatgtatat
     2761 cataatttgc actaataaaa gtagtatcaa gaagcaaatc attttccaaa agattttcaa
     2821 ctaaaagttt atgatattta ttatctgttc taggtgtatt atgaattgct tcagccatta
     2881 gcagtattaa atattgtctt aaaaaacgta tactatcact tctgttattt agattttcta
     2941 gtccaaaatt tctaaatgct tcttttcttt gttctgctaa gtctaaaaga cctaaaactt
     3001 cttcaaaggt aggaaaattt gcattatcta tattatcttt tactatatca atattgaaca
     3061 tctgtttaaa gaaaatatac aattctctat tcattttggt attataattt gtgggattta
     3121 gttttttaaa atattcgcta aaaagttcat tttgaatagg tagattctct gctgctgaag
     3181 ctcctgctcc taaaaatatt gctattttca tatagttcct cctaatataa atattactcc
     3241 tatttttata tccattaaat atccatatca tttattttat gatacaacaa taaagctgtc
     3301 ttttactaga aaaaatccta ttacaagaca gccttatatt atattaaatc tttaataatt
     3361 cgtaaataga aagagcatat atttgagtag cttttataat atcctctact attaagtatt
     3421 cattagcttg gtgttctgtt tctggttttc ctggaaaaat agctccaaat gcaacacaat
     3481 tatctaaagc tcttgcgtat gttgctcctc cagatgaaag tggtgttcca tcaagaccag
     3541 tttcttcttc atatactttt ctaagtgtct taacaagaag agtatcttct ggaacataga
     3601 ttgaatccaa gaaatcatat tcttcataat ttaaattgta tttatctgtc attttcttta
     3661 actcttttac aaaatcatcc tttttgtatg ttactggaat ccttacatct attcctgcaa
     3721 attctttttc gttatcaata gttactctac ctatatttac tgttagttta cctgatacat
     3781 catccttgca attaggaatt atattgtttc cattagcatc ttcaccaata acttctgcta
     3841 aaaacttaat tatattagaa tctataccta tattattaag agctatacaa agtctagcca
     3901 ctgcatttat accagtatca cttgctgctg aatggacact ttttccaatt atacagattt
     3961 tattaccttc aacagtatac tcaaaattta atttatcaag ttcttttttt aatttatcag
     4021 agtattttcc aatatagatt gctttacctg gaactgcatt taaagccttt ccaactgaca
     4081 actctatatc acttttgcta tcacaagtca aatgaacttg taaaagacct ttctctgcat
     4141 ttgttattgg gaatctagaa tctggagtaa atccatagtt tggtatttct tcattatttt
     4201 ctttatattt atttatacat ctccaaagat tttcttcatc agccccaaaa atgaatctta
     4261 tcctcttatt aaagtctaca tttaaatcca taagagcttt cactgcataa attgctgata
     4321 ttgtaggtcc tttatcatct tgtgttcctc ttccatacaa tttaccatct tctaaaacag
     4381 cttcaaatgg agggtaattc cagctttcta aatcaccttc tggaaccaca tcaacgtgac
     4441 caagtatacc tatcattttt tcaccttgtc ctatttctgc atatccataa tatccatctt
     4501 tgtatactgt cttaaatcct aaagatttac ataagtctaa tatacctctt aaagctttat
     4561 ctacattttc accaaatggg caattttcag ttgcttcaga tataacacta ggtattttta
     4621 cactttcttg tattgaagaa atcatttcat cttgtagaga atttactttt tctttaattt
     4681 gctctttcat attatcaccc ttcaattttc atcataatat actaataaat attttgacct
     4741 ttttaaaaaa aaatatgctt attttttcag tttttttata aattcaatcg cttttaaagt
     4801 ccccattttt gaatggtcaa atgtaagtcc accttgaaaa taaacattat aaggaggtct
     4861 tattggagca tctgcactta attctataga agaaccttgt ataaatgctc ctgctgccat
     4921 tatgacttca ctttcgtatc caggcatgtc ccaaggaaca ggttttacat aagaatcgac
     4981 tggtgcagct tcttgtatac cttcacaaaa acttattact tcatcagcat tattaagtct
     5041 aatacattga ataatatcac ttcttaagtc atcatatttt ggtaatacat catatcctaa
     5101 tttttcaaaa gctcttgagc aaaagatagc tcccattaca gcctgagata ctatataagg
     5161 cgccaagaaa aaaccctgaa gtacatttct agttgttcca aaagtaagac cacattcttt
     5221 tcctattcca ggagaagtca ttctataaga tataagctca atcaaatctt ttctaccagc
     5281 tatatatcca cctgtcaatg caagcccacc accaggattt tttattaaag agccagccat
     5341 aacatctgct ccaacatcag taggctcttt ggtatctaaa aactcaccat aacaattatc
     5401 aaccattact atagcttctg gtttaacaga ctttattact tctatagctt cttttatatc
     5461 actaattgaa agtgattttc tccaagaata tccttttgac ctttgtatca taacgagttt
     5521 agttctatca tttattttgt ttttaattcc ttctaaatct aagtttccat cctctaaaaa
     5581 atctacatca tcatatgtaa caccatactc tttcaatgaa cctttttctt ctcttatgcc
     5641 tataactccc tctaaagtat catatggtct accagtaaca gataaaattt catctcctgg
     5701 tctaactata ccttgtatac ataaagtaag tgcatgtgta ccatttacaa ttattggtct
     5761 tacaagagca tcttctgtat taaatacttt agaataaatt tcctctattt tttcacgacc
     5821 aatatcatta tatccatatc ctgtagtcca gttaaagtgc atatcactta gtttagattc
     5881 ttgcatagct tttaaaactt tatattgatt atattctctt atttctttta tttcttcaaa
     5941 cttatctttt atttcttcca ttatttcttg cgatagttta aaagtatcat catctattcc
     6001 atagtagtct tttaataatt cttttgtctc attaagcatt ttatcacctg tctctttatt
     6061 attttattta aacatgtaaa ggagactatt ttactaaaac aaataaatta tagtttataa
     6121 gttagtataa gtctcctctc aatcatctta aattaaatta attatctatc tgttgttatt
     6181 attattgttg ttttggtttt gatttgttag attaatatac tttccaggtt gtatagttga
     6241 tacagcatgt ttataaatca tgttttgttg tctattatcc ccttctatta atattatata
     6301 actatcaaac ccctttacaa gccctttaac ttgcactcca ttaactaaat atatagtaac
     6361 aggtatcctt tctttccttg cattatttaa aaacaaatct tgtaaattta aaactgtatt
     6421 tttcattaga tttgtaccct cccaagatta aattgaactt ctaaaatatt cctaccttat
     6481 attctatata agttatgaca aattttcaat attaagttgt atacttaaaa atttatatat
     6541 tattttatag aatctttaat atataagatt atttcatttt ttaattcgtc tatattttca
     6601 tattggtcta aatcaaacca tcttgctgtt ttatatcttt taaaccatgt tatctgacgt
     6661 tttgcatacc ttctagaatc tcttttaatt atttcaatag cttcttcgta tgtatattct
     6721 ccatccagat acttcaatat ctctttataa cctatacctt gcattgatat catatctttt
     6781 ttaaatccca tacttaaaag tttttttacc tcttcaacca atccattttt aatcataata
     6841 tcaactctca tatttattct ttgatacaaa tgttctctat ctctatttag aactattatt
     6901 attggttgat attcttcatt aaattttaaa tcacttgaaa aatcattcat tttcttacca
     6961 cttagacaaa cttcaagagc tcttatgacc cttttagtat tgtttttatg gattctatta
     7021 gcagattctt catcaagttc ttttaactta ttatgcatgt aatctattcc cttttcagca
     7081 agctgttttt gaagttcttc tcttaattca ttattggcat cagattgtgc aaaatccata
     7141 ttatatatta aagagtttaa ataaagtcct gtgcctccag ttactaaaac acattttccc
     7201 ttttcattta tttcatgtat atattttttt gctctttgtt gaaactctga aactgaaaat
     7261 ggatagtcag gcttaacttc atctataagg taatgcttca ctccttgcat ttcttcctct
     7321 gtaacttttg cacttccaat atccatatat tcatatattt gcatagaatc agctgaaatt
     7381 atctctgcat ctatatcttt tgctaatttt attgacaaat ctgtttttcc aacagcagtt
     7441 ggacctgtaa gtattataag tggtattttt ttcataacat tcctttccat atattttaaa
     7501 tatccaaata ttttacataa ttcttttaaa cattttttct atttctgtct tagatatttc
     7561 gaccattata ggtcttccat gagggcatgt aaatggattc tcacactttt ctagctgttc
     7621 caataaactt tttatttcaa tatcgtatat tttatcattt gcttttatag cagacctaca
     7681 tgccatagat gcaaatctct ctccttttaa atcataatta cttgtaattt cttctatatt
     7741 atcaattatt tgaagtataa atttctcagt ttcaggtact ccaaatatag taggaacaca
     7801 tcttaccatt atatgattat tgccaaaaat ttctagttca aaaccaaatt tcataaataa
     7861 ctccaagtta ttttcaattt gaagcatatc tacatttgat acttcaataa ccactgggtc
     7921 taaaagtatt tgcatattga tatcttgtct atagaacttc tccatatatc tttcatatag
     7981 tattctttca tgtgcagcat gttggtccaa caaatacata gaatcgtctt tgctcaaaat
     8041 tatataagta ttaaatacaa ctccaattac agaatatcca tataaggaaa acttgcgttt
     8101 tgaactacac aagtattctt cttcactgtc cttaatacta tctcctaagt aataatttcc
     8161 ttcattttta attccatcaa cttgaaactc ttcttgtata ttatcatttg cactatcttc
     8221 acttagataa ttaatgtttt ttacatcatc ttctacactt gcatttagga cttcacttac
     8281 tgattgaaat tcatttattg gtttttcact tgataaagtc acaacttcaa tgacttcatc
     8341 cttatcttta gtaatattac tatttttagg agtagattct aataaatcat tatttcttaa
     8401 tttataatct gtactttttt ctctactgtt aatagcgatt ctaggttgtg tttttttatc
     8461 actataagta gcatattttc caataagatt tgagtgtatt aatttaacct ttaaaaaatc
     8521 tctaagttct atatacactt cttgttcttt ttcaaattta atttcaagtt tattggggtg
     8581 tatatttaca tctatacatg atgggtctac ttctatattt aagaagcaca ctgcatgttt
     8641 acctattgga attatagact tataactttc tgttattgca tctatgatta ttttactttt
     8701 tacaaatctt ttatttatat agatatgttg taagttttta tttgaacggt atatattgtt
     8761 attacctata tatccattca ttttaaagtg attgcactta aactctacat ctattatatt
     8821 ttccgttatt tcttttccat atatacttct aatagtgttt acaagttttc catcccctgg
     8881 agtatttaac atctgtttat tgttatttgt atatttaaat tgaatgtttg gattacctat
     8941 tgcaagttta tttatcaaat cacttatgtt tatagtttct gcatgagtag attttaaaaa
     9001 tttctgtctt gctggtgtat taaagaaaat atcttttata atgatggtag ttccattagt
     9061 agaacctatt ggttcttttg atattatttt tccgccttca acatatattt tagttcctat
     9121 tatttcatct ttagttttag tcgtcatttc taattttgat acagctgaaa tagatgccaa
     9181 tgcttctcct ctaaaaccaa gagagtataa atcatacaaa tcatcaattt ttttaatttt
     9241 actagtagca tgtctcaaaa atgatttttc aacttcactt gaaggtattc ctattccatt
     9301 atctgtaatc ttaattagac tttttcctcc atcaattata tctatggaaa ttttatttgc
     9361 acctgcatca atggagtttt caataagttc ttttactaca gaagaaggtc tttctacaac
     9421 ttcacctgct gctatcttat ttatagtcaa atcatctagg atgttaatta tatttttcat
     9481 ttttacacct ctaagatttt atttcttttg cttttctttg aagattataa agagagttta
     9541 tagcatctaa tggagtcata tttaaaatat ccagatttaa aatttcttct gatagtatat
     9601 ctctatttac actatcaaaa gagatttgag ttaaggctac ttcttttaca gaatcttctt
     9661 gattaatttt ctctttatca tcattttgct tcattaaagc aacttcatca tttaatgctt
     9721 taaatttaga atttaattta tcataatcct tttttatatg tttgtagtct tttactataa
     9781 gttcatgttc tatttttaga gattcatatt tattcttaaa attactttga tttaccttaa
     9841 ctaattcttc gtccaaatta ctattaatta tattattgtt ttctttatct ccatttattg
     9901 caacactgtt gtaaacatga tttttttcca agtctttaag tatatatttt gctctttcta
     9961 tgacttcgtc tggtaattta gctaatttag caacatatat tccataactc ttgtctgctc
    10021 cctgaggaat tatttttctt aaaaatatta ttccttcccc atcttctttg actgctattg
    10081 aataattttt aacctcttta aactcttctt ctaaatcagt aagttcatga taatgtgttg
    10141 caaataatgt cttacatcta atattttttt gaatatattc cactattgac catgctaaac
    10201 ttatcccatc ataggtacta gttccacgac ctatttcatc taatataact aaacttctct
    10261 ctgttgcatt ttttaatata agggatactt cattcatctc taccataaaa gtagattgac
    10321 cttgagataa atcgtcacta gctccaactc ttgtaaatat tctatctaat attggaatat
    10381 cagcagactc tgctggtaca aatgagccta tatgtgccat aagagctatt atagcagttt
    10441 gtctcatata tgttgattta cctgacatat taggaccagt tattatgtta ataatgtttt
    10501 ctccacgatt taaataagta tcatttggaa cgaaattctc ctctcctact atattttcga
    10561 caacaggatg tctaccattt cttatatcaa gtttattatt ttcatttatt gctggtttta
    10621 catagttatt tatatgtgca actgttgcaa gggacacaaa aacatctata ttagctatag
    10681 tcttagccac tttttgtatt ctatctatgt ttttatatat tgtatctctt atctctacaa
    10741 atatctcgta ttctaaagat ttaatctttt cctctgcatg tagtatcttt tcttctattt
    10801 cctttaattc tggagttata tatctttctg cattactaag tgtttgcttt cttatatatg
    10861 tctcatctag ctttgcctgt ttaaagttgg cttttgttat ttctatataa tatccaaaaa
    10921 ctttattaaa tcctattttt aatgatttaa ctcctgtttt ttctctttcc ctgttttcta
    10981 tttcctttac aagaaaggct ccattcttag aaatctctct tagttcctta agttcatcac
    11041 taaaatctga tttaattatg tttccatctt ttatggttat cgtaggttct tctagaatag
    11101 cttcatctat taaattgtat atatcatcta atttatccat ttctgaaaca tactctttta
    11161 ggatttttgc atttgataaa tttatagtat cttttaaatt aggtaacttt tctatcgaat
    11221 tttttaagtg aattaactct tttggtgtta ctctctcaaa tgctatcttt ccacatattc
    11281 tttctatatc gtatatattt ttaagtatat catttaggtc ttctcttaat ataaagtcat
    11341 cttttatttc ttctattaca tctaatctat tttcaatttt agatttattt atcaaaggtt
    11401 cttctacata ttttcttaaa agtctacctc ccattgctgt agaagtttta tcaagtacat
    11461 gtaataatga accttttttc ttattccctc ttatagtttg agtaagttct aaattagttc
    11521 tagtaaacat atctaaaacc atatattctg atgaattgta tatatttata ttattaatgt
    11581 tagatgttat ttgtttttga gtattgtata tataattcag aagaattgat aagcttgact
    11641 ttattagacc tttatcatca aactttagtt tttgtagata aacatctgga aaatattctt
    11701 ttaatatatt tatatccaaa tagttatcac taaaactttc atttatgtat atattactta
    11761 cagtagctat atctctaagc ttttctataa agtctaaatc atttataatt atttcagttg
    11821 gatggatttt tgcaatttct tcaattacct tatcttcatt taaacatgtt gcattagttt
    11881 ctcctgtact tatatcaaca taagttagac ctatattagt accatcctta tacaaagata
    11941 ataaataatt attcttttta ttttcaagta aatttccatc taaaactgta ccaggagtga
    12001 tgactcttat aacctctctt ctaacaattc cttttgctgt agatgggtct tccatttgtt
    12061 cacctattgc tactttatat ccattttcaa ctaattttga tatatatgaa ttggctgagt
    12121 ggaaaggaac accacacata ggagcacgtt cttctaaacc acaagctttt cctgtcaatg
    12181 ctatttcaag agcttttgaa gctactaatg catcttcaaa aaacatttca taaaaatctc
    12241 caagtctaaa aaacagtata cagtctttat atctattttt tacttcaaga tactgtttca
    12301 tcataggagt taatttattc atatctattt gcattatcaa acctccttct tttctaatta
    12361 aatattatat catagtagat gattattatt aacacaatat ataaaatttt gacatataac
    12421 taaaaacaat ccaaaatgta tactcacatt ttggattgct aagtttaatg cttattaaat
    12481 aaaaatatat atatttttac aataaagata ttatttcttt tttaagttta atgatatttt
    12541 caaggttatc ttcatttaac tttctactat ctataacaaa ttctttcttt actattgatg
    12601 gtttttttga aataacataa attctgtttg atagcattat agcttcgtca atatcatgag
    12661 ttataagaag tattgttgaa tttacttttt tcttaacatc taaaagccat ttttgcatgg
    12721 atgttttagt taatgaatca agtgccccaa aaggctcatc taaaagcatt atgtcattag
    12781 aatttataaa agttctcaag aagttagctc tctgtctcat tcctccagaa agttctgaag
    12841 gatatttatc ttcatatccg cttaatccaa acacctcaaa ataaggtttt accattgacc
    12901 tagctttact ctttttttct ttttttaaaa ttagtggtag tgacacatta tctattatag
    12961 ttttatatgg caagagtaaa tctttttgat acatataact atatttacca tttatattca
    13021 ctttgccact atcataatct gtaagtttag ttataatatt aaaaatagta ctttttccac
    13081 atccacttgg ccctaatata gaaacaaact caccttcata aacatcaata cttatatctt
    13141 ttaaaacttc aatatcttta taagtttttg atacattttc tatagatact tttacttttt
    13201 tattgtggta aaaattcatt tgtgtaagca tcacttgcct tcatatcctt atctattaat
    13261 ttatattctt ttaagaatga tgtatagttg ttccatacac tatctttcat ttctccccat
    13321 cgtggtgcat catctttgta cttgcttgct aaatattctt gactctttaa agctagtttt
    13381 tcatctactt caggtgcatg ttttactaaa atttttgctg attcttctgg attttttatt
    13441 gcatattcat aaccttttgt tgtagctttt aagaattttt tagcaagctc tggattatct
    13501 ttaagcacag tttcattaga aattataagt ggtgtataat aatccaatct ctcatctaaa
    13561 tcttttactg gtataaagtt taagtcatat ccaattaaat cagctttaac tgcatcccaa
    13621 ccttcaaata tccatgcaaa atctacagtt ttcatagctg caaagaaatc atcttgacca
    13681 gtattaacta tctttaactt attaaaatca gctccatttt tcttcataac tgctttaaaa
    13741 acagcttctt ctgatggaga gccccaacca ccataagttt ttccttcaaa atctttagct
    13801 gttgttatat tcttttcttt tggtgatgca aacccagaag tattatgttg tataacagtt
    13861 gctacagctt ttattggaag tggatcttca ctagtctttg cataagtaac ttgttcttga
    13921 tagcttatac caaagtcagc ttttcctgtt gctactaaag tttctgctcc actttcaggt
    13981 ggttgtacta tttcaacatc caaaccttct tctttatagt aacccttatc aagtgctaca
    14041 aataatccag tatgatttgt atttggtgtc caatcaagca ccatagttac ttttttagaa
    14101 gaagtatctt tatcattgtt tttgtcttta ttgttatttg aagaacatcc agttattgtt
    14161 gccaatgtaa aaacacttac taaacctaat attcctaact ttttaaaatt cataattgcc
    14221 tccctcaaat acctttaatc aattaatcta tgtcttataa caactttttt tacagcctgt
    14281 acaattccta caaacattaa actaaatata actactaata tagtagatgc aaatacttta
    14341 tcaagtgcat atgcactttt tgacctaagc atataaacac caagtccttt cgttccacct
    14401 agccattctg caacagttgc tgccattaca gcatacgttg cagatatttt aagtccagaa
    14461 aaaaatttat ccattgccat aggaaattta aggtgaatga atgtattaac tttatttgag
    14521 tccatagtct taaataaatt taaataatct ttatcaatat tctcaatgcc atctacaaaa
    14581 cttattaata taggaaaaaa acaagtaagc attaccatca atacttttgg cattgttcca
    14641 aatccaaacc atatcataaa aagtggtgct ataattattg taggtatcat ctgtgtaacc
    14701 aacataatag gataaagaca tttttttatt attgatacaa aatccatcaa tattccaatt
    14761 accaatgata ttataattgc gactataaaa ccaagtattg cttctcctat agtaaccaca
    14821 gcatgcatgg ataaattttg ataatcttta aaaaacacat taattatatc tactggtgtg
    14881 ggtagtatat actgaggaac ttctaaaact cctaccatag attgccaaag aataagtata
    14941 aacaaaaatg ttgctattgg atatatacta tctttgactt tatctatgat ttctaagttt
    15001 ttcatctata gtcagacctc cagctttttc atctactcta gctactgtta tgactctttc
    15061 acatccaaga ttaaaaggga ttttatgtac cttttttaat aattcaaaaa cttcatcaaa
    15121 atcaccttca acagtagtgc tcattgctcc tatttcacat ttaagaccac ttttttcaat
    15181 ttcagcaata caagcatcta ctactttata catttcttct tcacctttgt aaggtcttag
    15241 tggcattact gctatgtctg cgattaccat ttaaatcact cctttaaaaa taattaatac
    15301 ctactaggta tattttgttt tttattaaaa aattttatta ctaaaaagat attatattta
    15361 aataaaagaa aagtcttatc caaaatagga taagacttat ttttatctta actggtctcc
    15421 ctacactggc attatccaaa caggttcaaa gggtcgagat aaaatctcct ctcagcacat
    15481 tagctccccg aaatatatta tatcttttct acttgattat actacactta tcatatttta
    15541 acaaattttt tatttatatt agatatttta tccgttcaaa aatatctaat atacattgta
    15601 cattattaca ccaaataaat tatcttaaaa tggtaattta tcaacatctt ccaaaactag
    15661 attaactgaa ttttctagtt tttattttgc tatttcaagc atatttggat gtatatcaat
    15721 tccatacaaa tattgttcta aatttctgtt ttcactaatc taatttgcaa tcgttacaga
    15781 ttacagatta gaaatagctt ttagactcta cttatcttta tctacaaaaa atccttcaat
    15841 aaattcagta ataatattca gtaatctaaa aataaacctc tatctggatt tttaagatac
    15901 aagcctttta ggaagtatct tttgtgataa tataaaaatt actcgtcatt atcatcaaaa
    15961 tcctcagcaa ccaacatttt agataagaat caaactctat aatataatta ttttatccaa
    16021 tttatttttg agaaataaaa tattttttta atttttaaat tttttaatat gcaccttgaa
    16081 aaaagaaacg ttttcctata ttatggtaat agtagaataa aactaaatag gcaaaactag
    16141 agaaatttag tgacgcaaag ctatagggac taagacttat gaaatgagtt atgtcagcca
    16201 gttgccaaag agatattttc tttttgtttt tatgaatgtt ttttaggggg atatatgata
    16261 tatgtttatg gataaaatgg atagatgtac acatatacta actgcatata tatgcagttc
    16321 acgcgattac tgcaatttta tagatacaca attaaatgat tttatactag aatatggaga
    16381 gaatgtggta gaatcttgtt tgcatcaagt gatggtattg gtaagcagat ataattaaag
    16441 aaattttatt ttctagaaaa ccctttttag ttttatctac gttttataat tcattaaaga
    16501 actaatcaaa ataagcttca ttatatattt ttcatgaagt aaacagttga gtttatctca
    16561 tatactgttt accccaatta cataattcta ataatataga ttccaatgat ttaccacgtt
    16621 tagtagacaa atattcaact tttggaggaa tagtcggata agaaattctc ttaataagtc
    16681 catctctttc aagatacttt aattcttgtg acagtatctt atgacttata tgaacaacat
    16741 ctttttttaa ttgactgtaa cgtattggct cttgttttaa aatatgccat aaaataattg
    16801 ccctccattt acttcctatc acagtcatgg taaaaccaat tgaacaatta aaatcttttt
    16861 gtctttcttc aatatttgga aattcttcaa aattattatc tatatccatc tcaatcctct
    16921 ctttcaaaat agtaagtatc taactaaaaa gtgcattctt gtttagcgta ctaattcgta
    16981 atataatctt gattaaaaaa tagctacgca cactcgtgac tttagtcatg agttagtagc
    17041 taaaatagtc aacatatagg gaaac
//
LOCUS       DAMKPG010000079        17056 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMKPG010000079 DAMKPG010000000
VERSION     DAMKPG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.083"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(132..656)
                     /locus_tag="PC621_002752"
     CDS             complement(132..656)
                     /locus_tag="PC621_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF4109899.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(721..1473)
                     /locus_tag="PC621_002753"
     CDS             complement(721..1473)
                     /locus_tag="PC621_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF4109900.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1504..2802)
                     /locus_tag="PC621_002754"
     CDS             complement(1504..2802)
                     /locus_tag="PC621_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF4109901.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2819..5083)
                     /locus_tag="PC621_002755"
     CDS             complement(2819..5083)
                     /locus_tag="PC621_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF4109902.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5338..6156)
                     /locus_tag="PC621_002756"
     CDS             complement(5338..6156)
                     /locus_tag="PC621_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF4109903.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6149..6847)
                     /gene="cbiQ"
                     /locus_tag="PC621_002757"
     CDS             complement(6149..6847)
                     /gene="cbiQ"
                     /locus_tag="PC621_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF4109904.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6850..7134)
                     /locus_tag="PC621_002758"
     CDS             complement(6850..7134)
                     /locus_tag="PC621_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4109905.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(7124..7876)
                     /locus_tag="PC621_002759"
     CDS             complement(7124..7876)
                     /locus_tag="PC621_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF4109906.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8065..8257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8389..10161)
                     /locus_tag="PC621_002760"
     CDS             complement(8389..10161)
                     /locus_tag="PC621_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF4109907.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10175..11104)
                     /locus_tag="PC621_002761"
     CDS             complement(10175..11104)
                     /locus_tag="PC621_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4109908.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11333..11521
                     /locus_tag="PC621_002762"
     CDS             11333..11521
                     /locus_tag="PC621_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4109909.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            11514..12215
                     /locus_tag="PC621_002763"
     CDS             11514..12215
                     /locus_tag="PC621_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109910.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12217..13140
                     /locus_tag="PC621_002764"
     CDS             12217..13140
                     /locus_tag="PC621_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4109911.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13297..14220
                     /locus_tag="PC621_002765"
     CDS             13297..14220
                     /locus_tag="PC621_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109912.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14213..14950
                     /locus_tag="PC621_002766"
     CDS             14213..14950
                     /locus_tag="PC621_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109913.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            14961..15734
                     /locus_tag="PC621_002767"
     CDS             14961..15734
                     /locus_tag="PC621_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109914.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            15750..15902
                     /locus_tag="PC621_002768"
     CDS             15750..15902
                     /locus_tag="PC621_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109915.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(16275..16883)
                     /locus_tag="PC621_002769"
     CDS             complement(16275..16883)
                     /locus_tag="PC621_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF4109916.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5485 a         2972 c         2148 g         6451 t
ORIGIN      
        1 cacaatacaa ataaattaaa aagtgaaatg tatttgtcta aaaacataat aacatttcac
       61 tttatcctca tttttaagta atatatattt ttaattttta tttataactc aatctagttt
      121 ttatataagt tttagttttt ttctacatct gagtaatcta ttccaaaagt ttctacagta
      181 acagacttca taatttgtgg agtttgtggc ttatcagctg aatctgttct aactgatgct
      241 atttcatcaa ctgtatccat accttctatc actctaccaa aacctgcata ctgaccatct
      301 aagtgtggtg agtttttatg cattatgaaa aattgagaac cagctgaatt aggtgccata
      361 gttctagcca ttgatatgac tcctctttca tgttttaaat tgttagtaaa tccattacca
      421 ctaaattcac cttttattga gtgacctggg ccacccattc ctgtaccatt tgggcatcct
      481 ccttgtatca taaatcctgg tattactcta tgaaatatta ttccattgta atatccttca
      541 tttactaatg atacaaagtt tttaactgta tttggagcta tatttggata taactcaatt
      601 ttgatttctt ttccattttc catttctata gttactatag gatttttatt ttccataatt
      661 tattaccttc cttttcatta gttaaattac ataattacat ttattatatt atactctaaa
      721 ttaaagtcta tcaagtatat tttttcctat ttcgtatcct tcttgataaa tagttttaca
      781 aaactcatca tcaaagttaa gcgtatcttt aggagcaaat tgagtattag gtctaactac
      841 tattatttta tcttcatcat atactttttt aatatcctct ggcaactcat aattatattt
      901 tgtggatatt aaaataattt tatccacatt atccccaatt aatggttcaa ttaatgcact
      961 cctaattaaa cctccatcta atttgaaacc atcataaatt ccttcttgca catctttcac
     1021 aaattgttct ttaaatccaa gagttgcgtt tggatttgat ggtaacaacc cactgtatga
     1081 aatgctgata atggcttcat tacgaggcat ttttgatatg tcaacaatct tttctgatac
     1141 tactttatta tcaacttcaa cataattaac ataaaagttc atttcttcta tattactatc
     1201 atctagttcc cttaggttgt ctatagcagg agaattatcc acagtattat ttactatttt
     1261 tacatttcca ttctggatat cttttatatt tgtccacatt ttctccaagt tatcaacatt
     1321 ccctgtgtac aaataatatc catttattgc tcctattgat gtccctgaaa tcgaataaaa
     1381 tttatttatt cctccatcat acaaggcttt aactactcct gcttgataag ccccttttgc
     1441 tcctccacct tctaaacata atcctaattt catagtctca tctccaaatt gtttatctta
     1501 tatttatatt tcaaaaaact tactaaatgt tttatatata ctatagtggt cctcaacatt
     1561 tcctaattct cttatagagt gcattgctaa aattggactt cccacatcca cagatggtat
     1621 atctatatga gtagaagata ttggacctat agtacttcct cctctttcat ctgacctatt
     1681 aacaaattct tgatactcaa cattaacttc tctacatata tttttataaa tagctataga
     1741 atatccatca ctagtatatg cctgactagc atttatcttt ataactggac cttttcccat
     1801 tattggttta ttagttgggt catgcttttc tggaatgttt ggatgaatag catgagctaa
     1861 atctgatgat atcataaaag atgaataaat agactcaaag aatccctctc tatccttacc
     1921 cattgatatg caaattctct ccaatatatt taacaacatg tttgaatctg caccttgttt
     1981 agtagagcta ccaacttctt cattgtcaaa tacagccatt acatttacac cttgttgacc
     2041 tttagtatta acaagagcat tcaaactagc atgtaccatt gcaagattat cttgtctccc
     2101 aatagatata aattcatcat tagcccctat taaacttcct ttttcaaatt catataaaaa
     2161 taaatcaaaa tctataatat cttccatatt tacatctaag ttagatgcta tttgctttat
     2221 aagaaaatta tctttttcta atgtatcatt taacatccca actaaaggta acatatcttt
     2281 ttgcttatta tatgaatgac catcatttat acttctattt aaatgtattg ctagatttgg
     2341 tattatacaa attggctttt taaagtctat aagcctttca tttggtttta atatactatc
     2401 accttttaaa actactctac cagctatccc aagaggtcta tccaaccatg tacttaatat
     2461 tgctcctcca tagcattctg tatttaactt caaataagat ttttcagctt ctatttctgc
     2521 atttggttta atactaaatg taggagaatc tgaatgagag cctattattc taaatccatc
     2581 tctttctata tcttctgaat ttactacaaa agctactata gctgatagat tcttagtaac
     2641 ataatacttt ccaccaactc ttagattcca tctttgattc aaaactaatt cttcaaatcc
     2701 atttttatct aagatttcct tagtactctc gactgcatga aaagacgacg gactatcata
     2761 tatgaagtct attaagtttt tagcaaagtt tatactatcc attcctcatt tacctccatt
     2821 ataaaatatt tagtgttgta tattctttat ttattttttt aattccctgt tggtcaaaag
     2881 ctatagtaac atctgtacca attatagaaa ccactgtccc aaccccaaat ttaggatgat
     2941 gtaccttaga accaagtttt atatcatctg gattagtttc tttattagaa tcttttattg
     3001 tagcattaac tttatctgca actttagatt tgcttatagt actcatatac tttttagtat
     3061 atttatctaa catattataa cttgctttag aatagcttag ttcttttttt actttgtaca
     3121 atctttctat acattcttct ggcagttctt ccataaatct tgaagctatt gcaacatttg
     3181 ttttaccata taatgttctc ttctctgtca atgacatata tagctcttct ttagctctag
     3241 ttataccaac ataacaaagt cttctctctt cttctatttg tgaatcactc atagatttta
     3301 tagctcttga tataggaaat aacccttctt ccatacctgt taaaaacaca acagggaact
     3361 ctaaaccttt ggatgtatga attgtcataa gcgaaaccct atcattgtct tcttcatcat
     3421 ttgattcaga tgtaagtgct atacttgtta ggaatgtctc taagtcttta ttttcataag
     3481 tatcatcatt actactttca aactccaaag ctatagaaat aaattctctc aaattatcaa
     3541 ttctgtcttg agcttcttca cctttgcctg ttaaatcttt ttcattttta tttcttatct
     3601 ctacaagctc gtccatatat cctgtagtat ccaagacttt ttctataagt ttgctaacag
     3661 gataaacttc ttttatagtt ctaagagtcc ctataatatc aacaaatcca cttatacttg
     3721 cccttgcctt agtagatata tctgaatttg tttctatatc aattaatact gagtatatac
     3781 tttcctgttt taagttagct ctatcttcaa ttttttctat agttctaagc cctatacctc
     3841 ttctaggaac atttataatc ctttttatag atatatcatc ttgtggattt tgaataactc
     3901 ttaaatacgc caacaaatcc tttatttctt ttctctcgta gaattttgta ccaccatata
     3961 tattgtatgg aatttgactt ctatttaaag cgtcctctac aggtcttgcc tgtgcatttg
     4021 ctctataaag tactgcaaaa tccttataag gtctattttc ttttctagct atttgtgcaa
     4081 ttgaatcagc aatgaaatca gcctcttcta tttcgctacc agttaattgt atctttataa
     4141 gttctccttc ttttttttca ctccaaagct tttttctttt tctttctata ttattagata
     4201 taactttatt agctgcatct aatatcactt gtgtagacct ataattttgt tctaacttta
     4261 caacatgtac attgtcataa tctttctcaa attcaagtat gtttcttata tctgcgcctc
     4321 tccatccata aatactttgg tcatcatctc caacaacaca aatattttga tgttctcttg
     4381 ctaataattt tatcaattca tattgagctt tacttgtatc ttggtactca tctaccatta
     4441 tgtatctaaa tctacttctg taatatgcta atacttcatc attagccttg aaaagttcta
     4501 cagttttaag tattaaatca tcaaagtcaa gtgcactatt tctttttaaa cggtcttgat
     4561 ataacgcata aatatctgca atcttaacca ttctattatc tgccatgttg atatctttaa
     4621 actgctttgg agtataaagt ttgtcttttg ctcctgaaat tgcacttata actgcttttg
     4681 gttcaaacac tttatcactt aaatttaatt cttttagaca gtctttaacc aatgtaactt
     4741 ggtctgctga atcataaatt acaaaacttc tattgtaacc aatcttattt atatcttttc
     4801 ttagtatacg aacacagcaa gaatggaatg tacttatcca catatcctta gtttcaggac
     4861 ctatattttg ttcaactctc tctctcattt catttgcagc tttgtttgta aatgttatag
     4921 ctaatatatt tggtgcttgt acacctttat cttctataag atatgctatt cttgttgtta
     4981 ataccttagt tttacctgaa ccagcaccag ccagtataag tactggacct tcagtctttt
     5041 ctactgcttc tctttgagca ggatttaatg tatctaaatt catatttctc ctcctgttaa
     5101 cagccataaa tatattttaa tctaaaatat gaattacttc aaattataat tatgtattat
     5161 attaatagat tatttccaat atattacaat taaataacat ttttccatat aatttattta
     5221 attgtttaat aaaaaaaata accttatgat ttggggaatc ataaggcacc tttaaggggt
     5281 ctaggttaag ggttattata tataaatcta tttttttaaa ataggtttag ggcttaatta
     5341 tttatttgta ttaagttctc tttccttcca atacttatac aaatcttctt ctttcttaaa
     5401 taagggtaaa ttcatatttc tatgtacttt tactaaccat ggggattcta ggccagcttg
     5461 tataatgtta ttttcattta caaatacatt ttctgccttt ccttcatcaa ttaaaatccc
     5521 cttatccaat acatatatat aatcgcatat ttcatacatt aaattcatat catgactgga
     5581 tattactatt tttatattat ctttattcaa tccctttatt atatctacaa tagacctagt
     5641 acttactggg tctaaaccag ctgtaggctc atcaagcaat ataatttcat tttccattgc
     5701 tataactgat gctatcgcta cccttttttt ctgaccataa cttaaaaaat gtactggtct
     5761 atctataaaa tctattccat taacagctac tagagctttg tttattctct ctttaattat
     5821 tttttcatct ataccaatat ttctcattgc aaaggctata tcatcatata cttttgaata
     5881 aaaaatttgt ttttctgggt cttggaacac aatacctaca ttttttctaa ggttgtataa
     5941 acttctctta tcatatttta acttttcttc tttaaaaaga atttctccag atgtaggctt
     6001 taatataccc attaaattca taaataatgt tgattttcct gaaccatttg aacctattat
     6061 acctataaca tttccctttg aaaaatccat attaatattt aatagtgcat tggtattctt
     6121 ttcatattga taagttaaat tatttatttt aaacatcatt atcccctact atgtgaaatt
     6181 taccatcata caattttatg tctaatgata tacacatatc atcatacctt gtcataagtc
     6241 ttttgtacag catactgcca agaattccta atgatttata tgaagttttt aaatttatgt
     6301 acccaaatct tagttcttgt gacttatata tttctgaaaa ttcttctaaa aatatgaata
     6361 taaacctata tattaacatt gatagctcaa caaaagtatc aggcaaatgc atttttttaa
     6421 agaagaatat taattgatta aaaggtgttg taagtataca aaagtataca caagtcaaac
     6481 aagacatagc tctaaataac acatgaatag acatgtcaat agatgctttt gatattccta
     6541 cattgaaact aaaaatttca aaactatata ataagtctgc cttattaaaa gatatattta
     6601 caagagttat tcctatacta agaaataaaa agtacattgg tattctgagt aattttaaat
     6661 aattctttaa atctattcca gctacacaaa caactagaat actcattaaa atcataattc
     6721 cacttaaaac aaatatattt tttattacca tagaagcaat cagaaagatt actcctatgg
     6781 ctactttttt atttggattt actttactta atctatttgt atatgcatat ttatcaatta
     6841 taagcataat taacttctct ttcctttttg aacacctaat atgtacccta ttacaccagc
     6901 acctaacgct gcttgtgcag agaataataa actttctatt tctccacttg gtggctcata
     6961 tgggctacta aaccatggtt cataatctgg atttatctta gtaatttcac tctcagcttg
     7021 accatctgct cctccatatt cagctcccga attaacaagt aatgggaata tgataagagc
     7081 tactactaat aaaagtagta atatattagt tttcgtcttt gttttagcac tcattagctt
     7141 taactccttt atcattattt tctagtatgc ttttttctcc attttctgta attaaattat
     7201 atacaattac agtaagaata ccctctgcta ttgctattgg tatttgagtc ataaagaaaa
     7261 ttcctaaaaa cttaattgct gaagccatca ctccacctga tgggtctggg aaagccagtg
     7321 ctaactgtat cgaagttact gtatatgtag ccaaatctcc tattgctgct gcaagaaata
     7381 ctggcattgc attatttccg tcttttttct ttaatgcttt aaatattaag aatgatataa
     7441 ttggtcctat aatagccatt gaaaaagcat tagctcctaa agtagttatt cctccatgtg
     7501 ccaatagtaa tgcttggaat attagaacta tagttccaag aacaaacatt acacttggtc
     7561 caaacattat tgctccaaga cctactccag taggatgtga acaacttcct gttactgaag
     7621 gtatctttag tgctgacaat acaaatacaa atgctccaca cagcgctaat aatactttct
     7681 ttttagggtc ttgctttact atttttccta ttgattttag tcctactaaa aaaaatggta
     7741 taaatataac tccccatgct atagaccatt ttactggtaa gtatccttcc attatgtgca
     7801 ttgcattcga tgtaactgga gttaaaacga ttaacataag tgcagctata acccctaatt
     7861 tgatattttg tttcatttta ctatcccctc tctccctttc taataattaa aatataaata
     7921 atatatgtct gatttatttt aaaaataaaa ataaaaaagc ctaactcgat ggctaggctt
     7981 ctaattttcg ataatccaaa tacaaacaca taaaatgaac atatctgtat aattattata
     8041 agaaactttc tccatccccg aagaaattac tttaatattt taggcaggtc tcctgactta
     8101 gcttcttcct actcctcttc cttcccatga gatataaaaa atatcatcat agtggctaac
     8161 aaaagtttcg tctgcatcac agtagcgggg gctgtaaagg aatttaacct ttttccctat
     8221 taatcttgct taaagaacct ataatattat atatttaatt tttaaattat tcttaaatca
     8281 ttttctatat aaatcattgt ataccttttc tgtgaaaatt tctacatttt ttataaattt
     8341 tattcatttt tatattttta tgtatataaa ttcttttttg taagtttatt aatttctaat
     8401 tatcttcttt aaatacattg aaactacatc agaaaatcta gttatatctc ttgaatatat
     8461 aaaatcattt ccatatatta atctttcagc ttcaagttct gattcttttc ctgtaaatac
     8521 tcctagtact aatatatttt gctttctagc ttttctcact tcattggctg tatctctcaa
     8581 accaactata cccctataag ccttttctcc tcttaatgtt ctctcactac tttttcctat
     8641 ctttacatca tttggtcttc catcgctgag aactatcagg attttatttt cttcatctct
     8701 atttattaga ccactacata tagattttat tgccaaacca tctctattat taccagcaca
     8761 aaaatattca aatatatttt cactattttt tatactatca tcataatctt taaatctttt
     8821 taatattgta tagtctaaaa aactagaaaa tcccatcact ctatttggta tgccaacttc
     8881 tgttagagca tttgcaatta tgtaagcttg aattgccaca tttccctgat ttctactttg
     8941 agagccactt gcatccaata atatatcaat aacgtatttc cctttttcat tttctatatc
     9001 tttataaaat attttattat tattacttct accaactctc caagctctat ttgctaatat
     9061 agtaccacaa tcagaatata ctctactttt agcattttct tctattaaaa ttcttgacag
     9121 actttctttt aatctcttta tttgctgtct atacattcta gcatgttctc taaattttcc
     9181 taaattattt tctttttgtc ttgttacata ttttagttga aatacattat tacactcact
     9241 tctaagaact ccatctgtaa agtgtattct acaaccttca tgaacatttc tacaattttg
     9301 tgtctcaatt tttttaattt cacctttact caaataagtt tttccataat aatgttctat
     9361 ttttgaatat atcttatttg cagtgtcttc atcaacatat ataactcggt ctgctgaatt
     9421 gctacattta gaatcatcaa aatcccctac accttctaca agcatggatg aagatattga
     9481 atctatatca ctttcaataa tattttcttc ttcatcacta taaagttctt catacatgaa
     9541 atcagcaaat gtatcaaaat ttacttctac actttctaca ttattttcat caataagact
     9601 attttcttct ccatttaata tgtattcgaa ataagttaaa tatataacat ctaatgcttt
     9661 taaaatctcc atcatatctt gattggtatc tatatttctt atatcattaa ctattcttct
     9721 agtcatacta tctaatgctg ggtgcttgtc catattataa agtacaaatg tgtatttaat
     9781 tttatccata atattatcat tgtgtatttt ggtaaaacgt gaaacaatat ctttataagc
     9841 atttcttctt atgtcttcta tccctggcct atctttaatg accttttctt caactattgt
     9901 atttaatata acttgaatta gacccagtat aatatctttg ttgtatcctt ttcttattct
     9961 atttgtaaga tattttttaa ctatatccca atctacatac tttcttctag cacctgcaat
    10021 tattgcaaaa tatagaccta tttcttttga agagtaatct ttttctgata tatctatact
    10081 ttcattgtaa tcaccagcta ttgtccatga taagttatta gctctatttt caaattcata
    10141 atcatcatat atccatttca taatctcacc tatattaaat atgacttatt ggaaatacat
    10201 ctatactctc ccatttatta ggtattctag ttttaattac atctccaacc atttctttct
    10261 catatacatc aaaagtcttt cctgtaagtc ccatattaat tgcaagagtt ggttttaatc
    10321 ctcttcttat agtcttaaga gatgccataa gaccacgtaa atcaatagct ttactagata
    10381 tctctccatt ttgagacttc aattgtaaat ctaaaaatat tcctgcaaag tgtattaatt
    10441 tttcttcatc agcatctgaa aattcatttt ttaaaatcat cattaactta tcttcttcta
    10501 taggaggaat atctattacc ataaatctag ataccagtgc ttcattaagt tcttttgttc
    10561 ctgcatattc ataattcata gtcccaataa atcttgttgc tggatgtaga ttgactctct
    10621 catacccagg tacatcaatt attcttctat agtctaaagc tgaatgtacc acaactatag
    10681 catcattttt tgccatatta atttcatcaa atactccaaa acctccatta atagcacact
    10741 cataaactga gcctcttcta agtttaactt cattgtctat aaatgtatct gtacctataa
    10801 gtgttgaact gtctgtattt atatgaaatg atgtattcca ctgaggtcta ttaaatattt
    10861 cccctagatt atcagcaagt aaattttttc ccgttgcctt tggtcccgat aataatatat
    10921 tttcttcttc taaaatagct gctatacaca tagacaagat atctttacca taaaaataag
    10981 ttttagactt tgttactctg tattctaaat ctttttctaa accataataa tttctaaaat
    11041 acagtacatc ttcaataagt ttttcatcta ccccttgcac ccttagatta tctattatat
    11101 ccatattttt acccctttta tataaatagt atttgtttca tttcttatca ccattttagc
    11161 ataaaaggat ataagctaaa attgattttt taatctcccc cattttaagt aaaaccatgc
    11221 accattttat cactacaaaa ttaagagtac tacttactga tatttcttga ttttgttcgt
    11281 ctatacgtat ataatatatg ctgataaatt atgaggaaag gatgattgaa atatggaata
    11341 tatgtcttgc ccagaagtag caaaaaaatg gggcatttct gaaagaagag tacaaaaact
    11401 atgtgaagaa agtcgcatac caggagtttc aaaactcggg tatatgtggc tgataccaaa
    11461 gaacgcagaa aagcctgttg atggtagagc gaaaagaaga aaggaagtac aatatgaata
    11521 gcgttttgat tatagatgat gacaaagaac tttgtgctct tatgaaaaaa tgtgttgagc
    11581 aagaaaattt atctgctatt actgcacatt gtggtgcaga aggtctacat ttgctcgata
    11641 aaaatagaca cacactctct cttgtaattt tagacataat gatgccagat atagacggtt
    11701 ttcaagttct gaaaaaaatt agagaaacaa gtaatattcc agtaattatg cttactgcta
    11761 aaaatgacga ggatgataaa gtttccggac tacgtttagg tgctgacgat tacctaacaa
    11821 aaccgttcag cataaacgaa cttatggctc gtgtcaattc tcttattcgt cggtatacta
    11881 tgttaaaccc tgcttttata acagacacag attgcataat aataaatggc atgattattg
    11941 acaagacaaa ccgaattgtt tctatcaata atattcctgt tcaacttaca agcaaagaat
    12001 ttgatttact tgcttttctg gcttctaata agggacgggt atttaccaaa aaacagattt
    12061 atacacaagt atgggaagaa gaatatgctt ttgacgatag taacatcatg tctttcatca
    12121 gtaaattgag aaaaaagatt gaaccagacc ccaaccatcc attttatatt ttgacagttc
    12181 gtggtgtcgg ttatcgtttt aataaggagg cttaacatga atacaagtgt ctatctactt
    12241 ttgatattgg ctcttagcct gattattata gcgtaccttg tttctgtcct gctacgtgtc
    12301 cgaatacaac ttacacttat aaaagatgct ttagaggata taaaaaatgg aaaccttaat
    12361 cgccgtattt taacagatga aaacgacatt acaaaacaaa tttgctatga tattaacgaa
    12421 atagcaatca atagccaatt tcaacttatt cagcaaaaac aatcagaaca agcctacaaa
    12481 cagctaatga caagtctctc ccatgatgta aaaactccac ttgcttcttt ggtcggttat
    12541 ttggaagcag tagaaagtaa aattgttgta ggagaggaaa aagacgagta tatccatgtt
    12601 gcgtctaata aggctcatta cttaaagaat tttgtagaaa atctatttga atgggtaaaa
    12661 ttggattcta aggaacaagt ttttcatttt gatatttttg acttgaatga attatcccgt
    12721 aatattatat ctgattggat acctgtttta gaaagcagtc attttgaata tgaatttgat
    12781 ataccagaaa tagagtactt cttacgtata gacgcaaatg catatacccg tatcattaat
    12841 aacctattac aaaatattat tacccatagt agtggcaaca aaatgacatt gagaattttt
    12901 gaaaacaaag agcaagctca aattataata acagataatg ggaaaggtat atcttcagac
    12961 aatctcccac atatttttga aaggttgtac caatgcgacc attcacgagc ttcaaaggga
    13021 aatggtttag gcttggcgat tgcaaaggaa cttattaacg ctcataaggg aaatattacc
    13081 gccaacagca ctcccggtat gggaacagaa tttacaatat tgcttcctaa agccctgtaa
    13141 aagtacgagg ctttcttact ctaaaacaag ataaaagcaa gattatggca aggttttggc
    13201 aaggttcatg ttttataatt ctaattgtag ttgattttga cgttgtatca actatctttt
    13261 gtagaaacaa atttattaaa agtgaggaaa ttatatatga atgattttgt gattgaaaca
    13321 aagcaactta caaaaattta tgggaatcaa acagtagttg acaaagtcaa tctccatgta
    13381 aaaaaaggcc gaatatatgg tctgttagga cgcaacggag ctgggaaaac cacaattatg
    13441 aaaatgattt taggattgac ttcaatcacg tatggcgagg tagatgtatt tggagagaat
    13501 attaagggat gtgaaaaacg agtatatccc cgtatcggtg caattatcga aacgccagga
    13561 ttttatccta atctgacagg aacggagaac ttggagattt ttgcaaaact tcgtggtaca
    13621 gccgccccta attctgtaaa aagagcattg gagattgtag gcttacctta taaaaataaa
    13681 aagctgttta gtaaatattc acttggcatg aaacagcgac ttggtattgc caacgctatc
    13741 ctgcatgacc cggaactgtt agttttagat gagccaacca atggacttga ccctattggt
    13801 attgcggaga tgagaaagtt tattagggac ttgagcgttg agcatggaaa gacaattctt
    13861 atctccagtc atattctttc agaaatctca ttgttggctg atgatattgg aattatcgac
    13921 cacggtgttc ttttggaaga aagtagtatg agtgaactag aaaagaaaaa cagtaaatat
    13981 atcctgctac aagtttcaga tattccaaag acatcactta ttttagagcg tcaatttaac
    14041 acgaaggatt attccgttca agatgaccac actttacgcc tttataacac tactctggat
    14101 atgggtgaaa tcaataaagc acttgtaatg cagaatgtaa ctgttattag ttctcaactt
    14161 tgtaatgata ctttagagga ctactttaaa aaaatcacag gaggagaggg cattgcttaa
    14221 acttattcaa acagaatttc ttaaactgcg tcgtaggaaa cttatttggc tgatgttgtt
    14281 gtctgctctc attatgccgt ttttcgcact actatacttc aattattttg gtaaaacagg
    14341 tgttgagcca acaaagtttt ataaatggtc tgcatttggt tatacacttt ggattatttt
    14401 gcccgttgtt ttgggtatat tttgcaccat gcttatacac gaagaaaacc agtatgatat
    14461 gctcaaacag ctttggattg tacctattag taaaatggga tactttttta gtaaattttt
    14521 cgttgttttg atttactcta tttgttttat gctgattaca gccgttgctt ctgtgttatt
    14581 tagtgtactt tctggatatg tcgtatttag ctgggcaagc attttatatt tgttgaaaaa
    14641 atgtttagaa attggtgtaa tcactgcttt tgtaatgttg ccgattttgg caatcgctac
    14701 atctcaaaaa ggatacattc tcccaatttg tatcacattg gtatatgcct tttccggttt
    14761 tgttataatg acaataaata tgtatttaca cccattatcc agtatgggag ttattatcat
    14821 gagaaataaa gacattccag gtcttatatt ttcacaggaa attaatgtcc cattagcatt
    14881 tctttgtatt tgtatttgga gtattgtatc agtattgttt gcaagcattg cactaggaag
    14941 aagaaagtga ggtgtagata atgtggaaat tactttgggc tgaatgtcaa aaaattcgtc
    15001 gttcaaaaat tgtttggatt acattttttg catccattat gatagcagtc atagtttttg
    15061 caggagggca ggatgtatat aatgacccag atttacatta tggcttaaag agcgtttatg
    15121 acggttcacg gtatatagac aatgcaggtt ggtatatgga cgcggtacaa ccttggtcaa
    15181 ctttttttgt tcttcctgct gtcattgccc tattaggaag ctatattatt tgtcgtgaag
    15241 aagaggagga cactattaaa tccctgcgtc ttattcccat aaatgaaaca aaattaacca
    15301 tagcaaaaat gattattact tttatattca gtatacttct ttatttgtta ctctttacta
    15361 taacattttt gactgaatcc gttttacatt tttccgattt atcgacaaaa ctggttttaa
    15421 gttgcatgaa agagtatttt cttgatggca taggtgtatt ctttgcaatt tctcccatta
    15481 ttgccttggt atcgcgcatg aaaaaaggat attggcttgc attagtattc acagaagttt
    15541 attctattgc tggactattt gcgggtatgt caaatgtctt acaaaccttt tttcctattg
    15601 cagcaatatt taatatttct ggatactata taactactgg agaaaaaatt atggggagta
    15661 tcataagttt attactttgt gcctgccttt ctgcatttat actgaaaggg ctaaaacaca
    15721 atgaaaaaaa ttgaaatagg tatatcaaaa tgaaaaagat aaaaaaactg attattctaa
    15781 ttgtattctt gtttcttttt ggcatgttac actgttatac tgaatggaaa ttcaacaatg
    15841 acccacctaa aggggtaata gaaagaataa atgagtggac tgataagatt ggactggatt
    15901 agtaacttat gttaattata gtgaatgtaa tattagcaag ttattgtttc gtattttaat
    15961 actataactt taaatatttg gttttaatct tccgaacgaa tataaaaaaa ctgtcacaga
    16021 ggcaacatat ttttgaatat ttataaagtg gtagttttga ttttcaaagc tgccgccttt
    16081 tcttctatat aagcaaactt atcctcaaca aagtgtaaaa catggtacat tttaagaaaa
    16141 aagtaccaca gacacaagca ataaaagaaa cgccgagaac ctacaaaaac gtagtgctta
    16201 tgcagattta cgacgccttc tattttctca accgtcttaa tgaccttgta caaaagaaat
    16261 ttttctaata ttttttataa tcttatcttg tgatgttttt tatgtataaa tgacttaata
    16321 acaaatccaa taaaaattat ggatattggt ataattagaa cgaggaattt ataatcatta
    16381 taatgtgaca ttattcttac ccaactctca gaaaaaatat atcctatact gattaatgaa
    16441 gtattccata tggttattcc cacgatagat gatattacaa aagttataat attttgacct
    16501 gctatacctg ctataaaaga tatgtatgtt ctacataaag ggattaatct acatacacaa
    16561 acagaaatag aagagtattt attaaaataa tcttgagatg caaatattcc tttctttgcc
    16621 tttggacata tctctattat tttatttatg atagatttac ctccccaatt tcccactgta
    16681 taacaaaata tcgaaccaag tatacctgca attatactta aaattaaaat tgtcagaaat
    16741 ccaaaatgat ttcttgcagc aattgctcca cataaaggta aaacaacttc gctaggtaat
    16801 ggaaaacaag catactcaat cataattaat acaaatacag agattaatcc atattgtaaa
    16861 ataaaactat tgattacaga cacttactta tctatctcct ttcttattta aaggttttaa
    16921 gggtacatat tctaaggatt ctcaccctat atttaaagaa tcaatctctt taaactatta
    16981 tatgtattta cacaaagcat ttataacata ctttaaaaat agtaccttaa aataagaaac
    17041 ttaataaata aaaaaa
//
LOCUS       DAMKPG010000080        16458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMKPG010000080 DAMKPG010000000
VERSION     DAMKPG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.084"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(91..1377)
                     /locus_tag="PC621_002770"
     CDS             complement(91..1377)
                     /locus_tag="PC621_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF4109917.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1500..4598)
                     /locus_tag="PC621_002771"
     CDS             complement(1500..4598)
                     /locus_tag="PC621_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF4109918.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5322..5474
                     /locus_tag="PC621_002772"
     CDS             5322..5474
                     /locus_tag="PC621_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109919.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(5766..6470)
                     /locus_tag="PC621_002773"
     CDS             complement(5766..6470)
                     /locus_tag="PC621_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4109920.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6461..8068)
                     /locus_tag="PC621_002774"
     CDS             complement(6461..8068)
                     /locus_tag="PC621_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF4109921.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8322..9500)
                     /locus_tag="PC621_002775"
     CDS             complement(8322..9500)
                     /locus_tag="PC621_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109922.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9726..10220)
                     /locus_tag="PC621_002776"
     CDS             complement(9726..10220)
                     /locus_tag="PC621_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF4109923.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10221..10982)
                     /locus_tag="PC621_002777"
     CDS             complement(10221..10982)
                     /locus_tag="PC621_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF4109924.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            11705..14539
                     /locus_tag="PC621_002778"
     CDS             11705..14539
                     /locus_tag="PC621_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4109925.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14582..15826
                     /locus_tag="PC621_002779"
     CDS             14582..15826
                     /locus_tag="PC621_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF4109926.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(16267..>16458)
                     /locus_tag="PC621_002780"
     CDS             complement(16267..>16458)
                     /locus_tag="PC621_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDF4109927.1"
                     /translation="VGSKATYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEA
                     GKVPTTIALMLSRILGFMK"
BASE COUNT         5732 a         2732 c         1882 g         6112 t
ORIGIN      
        1 tttttttcat gataagcttt aatactttat atagtaattt gttgtagaat tttataaaat
       61 ataatgattt atacagtttt ataaaatatc ttaaaacagt atttcagtaa tattttttgg
      121 tcttcctcca ccaatactag tctcttttgc acatattcga cctaaacctg catttactat
      181 tttatttaat atacgtcttg cacttctttc acttacattt agatactctg ctaactcttt
      241 tgaatcataa atcttacttt ttctattctc acttatagca ataatttttg caactgactc
      301 acaactcatt ccagtctttt tagatatttc tattaccttt tcatcagata gcatcaaaga
      361 ataatttaat tcattttcgg aagctaaagg tcctcttata ttttcatctt catctaccaa
      421 atatatacca atttcttttg aatctatgga gtgtcttaat gctttatgtg cattattttc
      481 agcctgatac gcagttactc cagtaccaat tcctatacta agagaaaagc ccatgtcttt
      541 tatctctctc tttagattga atagcttatc ataattatac tcattatcag ctaccccttt
      601 atgaacaaat acaacataat catttcttcc aagtaaaaat aaggaacctt gaatgcttct
      661 tacatagttt actacaagct tatccatatc tgattttttt atcatgtcag aataatagtt
      721 atctatcttc tctttataat ctattagact caatatttcg acagcaattt gagaaaatct
      781 tgccttgctt agagcatatt cactctttac cttatcataa ctttctttga ttaattgtat
      841 agtagcttgt aaacgaaata caggatagcc tctttctttt aattcattat atactgcacc
      901 aaaaccagtt aacattatat ccgtcttatt ttcttcaaat aattttatat gccattctac
      961 aagttcagac tctcctactt ccaaagaaaa tggattacaa aatacactcg ttgctttaat
     1021 atcaaattct ttaagtgcat cttccacaac atatctatca actacatcta tactgaattt
     1081 atctaatttt atatttgcac tttttattgc ccaaaatgcc ttcattatac tcgtcccact
     1141 tctcggtaca aatgaatgtg gcttacttat ctcatactta gattttataa attcataaac
     1201 tgcacatcct gtaaatataa tagcatcaca ttcatcttca catttagaaa taacttctat
     1261 tgtatctact actttttctc ttacatatat tttagtcttt aaatcactgt ctatctgttt
     1321 taaatcatcc tttatcttaa acccagagtc tgaaggacct ataataccca ctatcataaa
     1381 cttcgctcct aatccataaa tattatactt tttaaaaatt atatcctcac tattttctac
     1441 ttctatttaa ttatgttgaa ctactccatc ctacctacat ttagagtggg ataattagct
     1501 tatttttgat aaagtaactt ctcaaaatca gcacttggaa taggcttaca ataataataa
     1561 ccttgaatta ccacacaatc taaactcact aaaaattcag cttgttcttt tgtctctaca
     1621 ccttctgcca ctacatccat atctaaatct tttgccatgg ctatagtgtg ccttaaaact
     1681 attttttctc tgtctgtgat tccttcctca ttaaagaagc cacaatctat cttcaatgta
     1741 tcaatattaa tattttttaa catatttaat gaagaatatg ctgaaccaaa gtcatccata
     1801 tttaaaacaa taccatccat tctaaggtcg gctattatct tagtaaataa ttttatattt
     1861 ttaataaata aactttctgt aagctccaat tctaaaaaag attttggtat actatattta
     1921 tttattaaac ttaaaacaat ttctttaaaa tgtggattat gcaaatgtaa tcttgataca
     1981 tttacagaga taggaactgg ctcttcacct aaatctatcc attttctaat caaagaacat
     2041 acttgttccc acacatacat gtctaatctg ataataaacc cattcttctc aaatagtgaa
     2101 ataaatttaa ttggaggtat tatacctttt tctggatgaa cccatctaac caatgcttcc
     2161 gcaccaacaa ttttatttgc attaagatta tacttaggtt gtagatatac atggaattgc
     2221 ccattttcaa gtgcgtattc catttccctt tccatctgtt tttcttcaag tgcatcattt
     2281 cttatagtat catcataaaa tatatagtta ttgatacttt gtttctttac ttttttgtga
     2341 gcatatcctg ccatttcaca cgcatttgta attggaatat tattatccaa aattttataa
     2401 accccaaaac atggtactaa catatattca aatttttttc tatttaagct atttttaatc
     2461 ttataaatta atttatgaac atctctatca gagtaactct cataaaaaat acaaaataca
     2521 tctgcataca tacgacaaca tataccatta gaaccaatgt ttttttgaat caattctgca
     2581 acatatatta aagccttatc tcctcctcta actccataaa tgtcattaat taccttgaac
     2641 ttatctatat ccatcattat aattgcatag ctcttgtcag gatatctttt aagcattaaa
     2701 cttgtttgtt cacaaaaact ttcataattg tatagtcctg ttaatatatc cttttggttt
     2761 ttatgtagtt gcataagata atctatattt tttatagcaa ctaaaatatg tgtttgtttc
     2821 tcacctaact tatttttact actaatcttt aacatataaa ttaatgccca atagtacttt
     2881 ttatctattc ctaaacgtct atactcaaaa tttagttcta aatcgtcttg cttgctgtta
     2941 ttaagactat caagagattt tagaaggtgg ttatcatcaa ataatattat atcatcagga
     3001 tgaattaaat gtttttttat gtatgaaaaa gttttttcaa gattttcctt tataggaaaa
     3061 ctaaaaatat tgctctctga ttgataaatt ggagtaatta aattagtatc gatgcaaata
     3121 gataaaatta tatcatatgt attataagct aatttttcat aagccttaga ataccctttt
     3181 atcattaaat catcaaaata aaccttatca actaactctt tatgtttaaa taaaatatta
     3241 ttcgtcgaat cagctaagtc actaattact tccatttcat tatttctaag tttgttctta
     3301 ttataatacc tcttcttatc ttggtacatt ctctcatcag caactttaat tagcttatgt
     3361 atatcaatat tatcgtcact ccaaatactg cccactgcta cactatatat catctcatcc
     3421 ttaaattctt cacttaacca actcactaat tcttcaaata gttgttttga tatatcttca
     3481 cataatacaa taaactcatc tcctccaata cggtaaacat tataagaatg aaagtatttt
     3541 tttattatta cacatgctga cacaatagca gagtcgccat tagcatgtcc aaagttatca
     3601 ttaatatctt ttaacccatt tatatctaca aatacaactc ctatggattt aaattttcct
     3661 ttttgaaaat tatctatata cttaatatat ttgtttctat tatatgcacc agtcaagaag
     3721 tcatagtagc tcatataatt taatttattt tctacttttc tttttttcat ctcagttaat
     3781 aaaaaataag atagggatgt caaaagagag gtatctccaa cagccttatg tggattatca
     3841 actccaataa accctactac tttatcatct tgttttagtg aagatgtcat taaactttca
     3901 attttttgag gttttaaaat ttcatattct attggctgtt cttcttttat attttctata
     3961 tcatcaatta ttacaaaacc cttttgttcg aataataaaa accaattctt taattcatta
     4021 atatcaagat ttgttagtgc ctgtttttgt tcatcaacac cttcattgca ccattcatat
     4081 gtattagtta ctttttttct atcttctgat acttcaaaaa catatgctct atcagcttta
     4141 tgaaatttac caatattggt taatacaaga tttacagcat cagaaaaatt agattctgtt
     4201 ataagatgtt taatacatcc aactaataac tcctttatct ctaatttttc tgctattgac
     4261 ttacttatat cttccttttc tgtaatatca agagctattt ctaatcgtgc atgtttgccc
     4321 tcccatttaa ttaacttgtc ctttaatata aaatgtctat ttgttcgagt attcgtattt
     4381 tcccatatgt aatattcatc ttcttttaaa atagagtttg tacaaaattc gcagggagaa
     4441 tctttcccct gtaatgcttt ataacatttt acatgggata tatcattaat tcccattgcc
     4501 tttttcccaa tttcattaat atataaaagt tcatatgtat ctaaagaaga aacatataca
     4561 atttcatcca actcatctat taaatttatt acatccattt atatatatct ccttttattc
     4621 attttccaga ttgaattttt aaagctttta aaattttgct atatatttgg caatgatatt
     4681 atacttcttt atgttttaaa atagtacctt aataaagttc tattttttag taaatagaat
     4741 tattattgtg tcaatcgtac aatagagttt tgtaatacct aatttaatat atttataatt
     4801 aactatctta ttatgtgttt acaattaatt ctcatataaa tgacaaatct ccacctcaat
     4861 taagtattcc aatcatatat gaagataata cttcattcat ttaaaaatat aaaactatta
     4921 tatattaaaa tttcttcaat aataaattat ctagattgtt actttgatat attatataat
     4981 atcacaaagg ctgatataaa aagacaaaaa gggtaatttt tttatatcaa aaaaaatgtt
     5041 aaattgatat tatccatata taaattgata ttatccatat agatgctata tttaacaatt
     5101 ctacttactt cccttacact tattagcata tagttattaa gagtttgttg caaaacaaca
     5161 cttagcacac tttaaaaagt gtggtctaat aagtaatcaa cgaagaagac ataataaagc
     5221 ataaaatatg atatactttt cctgtcatca aaactgatga ttttactatt aatatctgct
     5281 aaaatatgct taataaatta aattttagtg ggagaatagt tatgaaaata tttagtttct
     5341 tgttgtttgt agtgggaata tgtttttgta ttgctggatt tatgggacat tacccagcat
     5401 ttgctggtgc aacattgagt ttttttgcaa ctacaatgat agctgtaaga caagtcaaaa
     5461 ctaagaaaaa atgaaatagg caaatcctaa ctttacaagt taatattaca taagtaatta
     5521 gtctaaaatt ggatactttt tctattacag aaatacatca tccactttgt attctcttaa
     5581 aaaaataaaa tagctatgta aattcaagat tagatagatt aactctctat ccatttcatt
     5641 gtaaaatata catagctatt tgtattcgat ttaaaatact tagattttct tacataacaa
     5701 ataatttcta tctgattatt actcatattc atttagtaat cactcagcaa ctaatcattt
     5761 agtatctatt catccgccat acggtaacct accccaacct cagtcaaaat atattctggc
     5821 tgagcaggat tcttttcaat tttccttctt atacttgcca taaaaactct aagcgactga
     5881 ttttcacttt catagtaatt tccccaaact ttatctatta taaacttatg agttaagact
     5941 cttcctgaat acttagacat caactcaata atcttatatt cgattggagt taaatgtatt
     6001 tcttcaccat taattgacac aatatgattt tcataatcta tctttaggtt tttaacttca
     6061 aaagactttg gagcatcatt atttatatta tcctgttgag ctttatttct aagagccaca
     6121 cgtactctag ccaaaagctc tgttacacta aaaggtttag ttaaataatc gtctgctcct
     6181 ccatctaaag cttccacctt atccctctca tgctctctcg ctgaaacaac aattatttta
     6241 ttagtctttg cacatgccct aactcgtcct attacctcaa tcccatcaat atctggtagt
     6301 cctaaatcca aaattatcaa atctgggtca agagaatgta taagtgatat agctgttcct
     6361 ccattatctg tctctacaca cctataattt tgagtttcaa gagatacttt tataaattta
     6421 caaattggct tatcatcttc tacaattaga actaatggct ttatttccac ttgtctttcc
     6481 cctcctcatc atacataggt atgctaaatc taaatgtagc tcctccagaa ggattattaa
     6541 atgcgacaat ttctccacca tgagcttcta ctatagattt acatatctcc aatccaagac
     6601 caatacctct tctttcaaga tttctagact tattcttagt ataaaaccta ttaaaaatat
     6661 taccgatatc ttcatttggt attccttttc cattatctgc aacttcaaat agcacttgat
     6721 ttgactcttt ttttacagtt agttctatct tagaattttt aggtgtatgc cttatagcat
     6781 tatcaattaa atttactaac acttgttcta ttaataatac atccacatga acgattatta
     6841 tctcatctgg aatattggtt tttatgtctc tactattggc aaactttttg actcttaata
     6901 tagattcagc tattatttca tcaacaactt ctggtcgttt ttcaatatcc aatttccctt
     6961 catctattct agtcatacta agtatattct caacagaatg agttagccaa ctcgtatctt
     7021 catatatatt tttcaataat tctactctag tttctttatc tatcacatca tcattttcaa
     7081 gtattgttga agacgaaccc aatatacttg taagtggagt tcttaaatca tgagaaattg
     7141 acctaagcaa atttcctctt aatctctctc tctctgcctc aagatttgct tttttatttt
     7201 tctcaaataa attttctcta tcaattgcca acgcaacctg tgttgaaact gcttctaata
     7261 gtatcttttc attttctgta ataatatcta tcttaaaaca cgcaacacct ataacaccaa
     7321 gaacatttga ttcttgacct tttataggga aataatatgc actgctgttt ttgcaatatt
     7381 ttgtcccaat tccaactggg ttacctgtcc taaaagcttc ttttatagct tctttttcta
     7441 atactgaatg gaatatattc tcacttccat cattgttaca aatataaata cttggcttac
     7501 ctagattatt attctcatct gcaatagtca ctattacagt tctatttaat atatttacta
     7561 aattagaccc acaaaattca ataatttgat ttttattttt tgctttaagc aacgttttgt
     7621 tattctcata gagcaatctt atcatttttt ctctcttatg agaaagtctc acttctcttt
     7681 ttatcctaga agttaaagta cttgttataa ttgctgccat tagcataata caaaaagtca
     7741 taggataatc ttttctataa gctaaaaaag tatataatgg atgcgtaaag aaaaaattaa
     7801 atgatagcac tcctattact gaagaaagta ttccaaaaaa atatccatag gttatacttg
     7861 tagcaaatag tacacctaat atgtatacta gtataatatt agattcatta aaacctacct
     7921 tttcaaaaac tactgatatt ccagtagcaa ttgtcataac caatatagtt tttatgaagt
     7981 ccttaataat tatcttttta ctgagatagc catcttcatc ataatcatac tcttttaagt
     8041 cttcatgttg tagtcgttcc tttttcattt actacaattc ctccttattg ttactgttta
     8101 aattggctct attttatata atacaatttt atcataataa attcaatcaa tacatttaat
     8161 taatgtactt aaattactaa aaggtgctgt cttaaaatga aaataaatct ctatttcaaa
     8221 acagcaccta tatatttaaa ttactaattt agtttttaca tacttaaatc aatagtttta
     8281 ttcaatttta taaattataa tacttcaatt atggaacatt tctatccaac aagcataaat
     8341 ataaagtggg cacatatacc tgcacacaaa ccacctatgg tatgcgaaag tatcgcttta
     8401 ccagccaatg gtcttgcatc taaagcatcc atcataccta tgtgtgtact taaatatcca
     8461 ctccaacaca tacccattgc tgtgaatact gctatatcat taggagttat agcgcctgat
     8521 tttataaact ctggtactaa agatattgct gctccaacag ctcctaatgc agttacaggg
     8581 aatgctattg cctctggtga tgtgaatcca aataatggtt ctattatgaa gctaatctta
     8641 tcaccaatag caggtaataa ttttatacct tcatatgcag ctcctgtgta aacagcttga
     8701 cctgtagcag ggtctgtaga tggtccaaaa gttaataaca taactaatgt acaaactact
     8761 aaaactcctg gtattatagc catacccatt tcaacaccta gcttaccacc ttcaagtata
     8821 gcatccaatg ttctttggaa tacattacca tctcttattt ctctaaactc ttcaagtttt
     8881 gcacctttat catttatttg cttttcagta tcatttttag ggtcataatt ataatacttt
     8941 ttagtaaaag tcaacataat tctaacacta ataatactac ctattattgc cccaacatta
     9001 cctattatag ctggtagtac atattcttta ccttgagcta tcataaaagt tgttacaata
     9061 agtcccatac caaaggctgt tcctaaatta cataaagcag gtacttgata cttcttaaaa
     9121 tattgtgtaa atgttttgtc tttagcaaat ggtataatag ctggattatc agataaatat
     9181 gttgcaacta ctcctgctat actagcacca ggtaagcccc atataggtct cataagaccc
     9241 ttaaatatct tattaactaa agatattact ccaaactctg aaagcaacgc actaagtgca
     9301 cctgcaagaa ctgccatagc cattattaaa aatactgtgt ctaataaaag cgcatgtgct
     9361 gtactcatta ttactttaaa catcattcct gcacccatga tgcttcctac atatccaaat
     9421 cctaccgcca atagtacaat aaatacgaag gtttccatac ttactgcctt gactcttctc
     9481 ttatccgaca gcttttccat cttgaaatct tccccctttt aattttaaat taaggttcct
     9541 aacattggta caaatttttt ggtacaaact gagttctaaa acttacactc gtaatgcaag
     9601 ttgtcagttt tttgaaataa aaaaaaatta cgtttataga ataagaatat cctctttaat
     9661 ttggatactc ttattctatt ttatgttatt atattatcat ccttttgttt aaaagtcaat
     9721 tttttctatt tttgttgttt aattttattt atactctttt tatatttttc attttctttt
     9781 atctttgtaa gcactccata taagttgact tttctaaata cagtagttat aaataccaat
     9841 cctaatgcaa ttgaacttgc acatgataat gtactcaccc catattctac agcttttata
     9901 taattattgg tcaaaacaga atacatagta ttatatattc cataaccagg tactaatatt
     9961 attaaacaac aaacacttaa tgtagttgct ggagtcatat aaattcttgc acataactca
    10021 caatatatcg caaaacaaac tgtagctata aaaaaacata agttttctgt tactcccata
    10081 tttaaaagaa atttatatga aaaccaagct atacttccac ctattcctgc tagtattaag
    10141 ttttttcctt tcatattaaa tagcactcca aagcttaagg cagtaaaaaa agctgctatt
    10201 acttctataa acaaatgcac ttatatcccc cctaacttga agtataatcc aagtaccaag
    10261 aacattccta ctgctataga tacacctaca aaaaatactt ctgctaattt tgcaagacca
    10321 gaaatcaact gtccatctaa caaatctctt aatgcattag tcaaagcaag cccgggaact
    10381 aagagcatta ttgctcctgc tattaaatgg tctaacgtct ttataaatcc gagctttaaa
    10441 attaaaattg aacaagaagc tataataact gctccaattg catttacaaa aaagtcatta
    10501 agtgaagatt tacttaaata cataataaca aacttagtca gaatcccaac agtaaatgca
    10561 gctataaatt cttccacatt tcctccaaat aatatcgaaa aagttgctgc tgcaatacct
    10621 gcaaaaaata ttgttattaa atccgagtaa tagttatctt cacttatttc atctaaaatt
    10681 ttctcacata attcaatatc taaattattt atacttattg ctcttgataa tgagttaact
    10741 tcatgaacca agtttaggtc tactcttctt gagcttattc ttttaattgc tgaatgtaat
    10801 ttcccatcta caaatacaga tgccattaca gctgttggag ttgcaaatac atctacgtgg
    10861 tctattccaa aagattgaca tattctagct atcgtttctt caactctata ggtttctcct
    10921 ccactttgca acatagcttt acctgcattt gaggaaaagt ttaaaattct atctatatcc
    10981 acaaaacata tctcccattc ttttatagta ttattcatat tttaatacaa gatattattt
    11041 atttaaacta ttttaaataa ttcatatcca atatttacat tttatgaaaa aacttgtttt
    11101 ttatgcttgt caaaatcaaa ttttaagctt gtttacacca taaaatggct gaattaatat
    11161 ttgacaaaca ctttcaacca tttatataac cttctaaatt gtataacttt ttactacaaa
    11221 taaaattata tctcttttaa ttctttctct tgagtattta agcttatagc aacttcatat
    11281 ttatatatgt acaatataaa acattatttt tatgatacaa aatataattt gtaaacgtat
    11341 atatgtatta actgtgtata tataaattat tcttaacctt atcagttttt tcatatatct
    11401 tcatctatat cgaccatata ttaattacat tgaccatata ctaataaatg atattacata
    11461 gactgaagta tattaaaaaa cttaaattta gttatttaac aacataatac tctaatttaa
    11521 tctataaaaa tccataccta atataatgca actcttattt taacaccatc tattacacaa
    11581 aacaacatat taaaaagtaa aatacaaaat atccactata aaccttattt taatactgaa
    11641 tatatattat aatatattta atgtagctta aatatagttt aaacataaag aaaggaaatg
    11701 gtatatgcta agaaaagata aagttctatt atctctacat aaattatgtg aaaatatgac
    11761 tttaaaagac cttcaatcag ataaaacagg attcactaca atagaaattg ctaaccattc
    11821 atctcttgat agaagtaata ccagtaaaga attaaataac ctatataatg aaaaaaaagt
    11881 tctgaaaata tctggtaagc caattttatt tctacatgtt agtattatag aaaatttaat
    11941 tggccgtact ttgaacagtg atgaattttc tataaataat tgtaatgagc tacttaaatc
    12001 aagtaaaaat gactcagaag atattttttc aacattactt ggacatgatt ctagtcttaa
    12061 actacctata gaaaaagcta aatctgctat actctatcct ccaaaaggtc ttaatacgtt
    12121 actcatagga cctactggtg ttggaaaaag tacatttgcc gaaacaatgt acaaatatgc
    12181 tgtagaaagt aaaatctttt cttctgaaag caaattcgta gtatttaact gtgctgaata
    12241 tgcagaaaat gctaacttat tattaagtaa tttatttggt tatgtcaaag gttcttttac
    12301 tggtgctaat aaggataaat tcggcatagt tgcacaggct gatggaggca tattattctt
    12361 agatgaaata cacagattac caccagaagg tcaagagatg ctttttcttt tgatggacaa
    12421 aggcatatac agacgattag gagaatctga cattgtgcac aaagctaatg tctttattgt
    12481 gtgtgccaca acagaagata tacattcttc tctgcttggg acttttttaa gaagaatccc
    12541 tgttacaatc aacctacctg ctttacagga taggtcttta aaagaaagat tatctcttat
    12601 taaatacttc tttattatag aatcaaaata caccaatgca actataaaag ttcacaaaga
    12661 tgtaattaaa tcattactcc tttatgattg ttttggtaat ataggtcagc ttaaatctga
    12721 catacaactt atgagtgcaa gagccttttt aaattacaaa actggactta aggataatat
    12781 agaaatagat ttatcccttg ttcctgatta catctatatt ggtcttttaa aattgaatga
    12841 aaatcgtagt aatctaaata aattatcaga acttgattat atgctttttt atgaatttaa
    12901 tggtaataac ttagaagttg aatatacaaa agaactatct aaatacaata tagatacatc
    12961 aaacaatgga tttaaatact ctacagagat tccttatctg gaaaatgata taaggagtat
    13021 gtttataaat tacatggaaa agtactctac aaaattactt tcgatatctt cagtaactca
    13081 ttctgattat acaagtaatg aaatttttaa agtaatcaat ccaaagattt actatgctgt
    13141 tgaacattct ttaaaagagg ccgaaaaaat atcgagaaac aaatacacaa aacaaactta
    13201 tattgctctt tcaatgcata taagtgcact tatagaaaat ataaatcaag acacctataa
    13261 tcctaagtac aatttatata atgataaaat tttcttagat aaagatttaa atatatctaa
    13321 aatagtttta agtataattg aaaatgactt acaaacatct ttacctaaag atgaagtaaa
    13381 atttatatcc atgtttctaa atagcatgta tcttgaacca aacaatcaat ctgaaagtat
    13441 aggagtcata atacttgctc atggaaattc aacagcaagc agtatgtgtg atgtagcaaa
    13501 ttcattgctt ggtacaaacc attgtaatgc aattgacatg cctcttgatg taaaagtaag
    13561 taccatactt gataaaacta tagagttggt aaaagaatgt gacgaaggta agggtgtact
    13621 tcttttaaca gatatgggct ctcttatagc ttttggagaa cttatttcag aaaaaacaaa
    13681 aatcaaaact cgctcaattg aaatggtatc tactccaata gttttagaag cagtaagaaa
    13741 aagtacatta ccagaaatga ctttggatga attagctaat tccttaagct ctttaaatcc
    13801 atatattgga cgagcagcaa tcgattcaac agaaataaac tatcctgaaa agtcaagaga
    13861 gtatgtaata ataactactt gtataacagg atatggttca gctatgaaag tagcagactt
    13921 tataaattct tcacttaaaa atattcgtaa atacaatatt aatttagttc ctcataatat
    13981 agaaagtttt aaaatgtata agaataaatt tagtaatact aaaattctgg cagttgttgg
    14041 aagtgtagat tttaatgtag ccaacacccc ttttatacac atagaggaat tactaaatgg
    14101 atatggccta gatatgctaa acaaaataat agaaggtaca ttcaatgcca atagtattac
    14161 aaatataaaa gaaacaaatc tatcatacaa cagtttaata ataaacttta tgacccaaaa
    14221 cttagaactt ttggactctg ttaaattatt caactatgtc aattcttcct tatttacaat
    14281 aatagaatta atagatgaaa acttaaagag caaatttaag ctaggatata tactacattg
    14341 ctcttttatg atagaaagat gttttaaaaa tacaactttt ccatataata acattactga
    14401 attaattagt aaaaatcatg aactatatag tttaatacgt gaagccttta agccaacaga
    14461 agcttatttt aatattttga taccagacga agaaattgct tatataatag acatgattaa
    14521 tgagtatcta aataaataaa atttatagca cttattttgt gcatattaat taatatatga
    14581 aatggaggac gagattttga aaggaaaaat aaaacagtta acaatacttg cattaatttt
    14641 tatatttata actcctgtat ttgcatttgc agatactcca ccagtaccaa attcttcaag
    14701 agcagcacta cttatagacc aggaaactaa aagaatatta tttgaaaaaa acattgatga
    14761 aaaaatgcca ttagcgagtt taagtaaaat gatgaccttt ttattggcaa tagaagcagt
    14821 tgataagaat caagtaaaag agactgatat ggtgaaaata gacaagtcta cagcttcagt
    14881 aggaggttct acttgtaaac ttaaagatgg tgatgaaata tctctaggag agcttatgca
    14941 gggtctaatg ctagtatctg gaaatgatgc tgctatagcc atagctaaac atattggtaa
    15001 gactgaaaag aattttgtaa atatgatgaa caagaaagca gaagaaatag gaatgattga
    15061 tacgtattat tttaatccaa atgggcttcc tatatatact gacccagaac ataaagaacc
    15121 tcctattgaa aatatgtcaa ctgctcacga tatagttaca cttggaaagt atatgtatga
    15181 ccattatgaa aatcaagtta caagaataac cactatgcaa gtatataatg atactaaaaa
    15241 ggattttaca cattataaca caaatcccct acttgtttca gtacctggtg ttgatggaat
    15301 aaagacaggc tatacagata atgctggata ttgccttgca ttttctatga tggtacctaa
    15361 agatgctaaa aacgaaagaa atcatagatt gataggtgtt gtacttggtg atggtaacaa
    15421 gaaaaataga atttcttctt ccgctacttt attgaaatat ggtaaagata atttccattc
    15481 taaaaagata gcacataagg gtgatattat agagactcct tgtgttgatg gtattgatga
    15541 ttttaagata acagttaagg ttgataaaga tttgtatggt gttgtttcag ataacgaaaa
    15601 tatcaatcct aaggtagtgt ttaaaaatat gaattatcct atccataagg gagatattgt
    15661 tggtgttgca aagtattata atgactctgg taaatttgtt ggaagtgttg atgtaaaaag
    15721 tgaaagtaat ataggatgta ttcctttgaa ggataagatt aagattaagg ttgctaagat
    15781 aaataaaaaa cttgagatta aaaattctgt ttgttttaaa gtatagtaaa gcatactatt
    15841 aaaagagtat attaaattta taaatactcc actttgtgga gtttttttgt tttttacaaa
    15901 aaattaaaat agtggcaaca attgaggatg tgagggagag ttgccactat tttgtatagg
    15961 gtattatatt atgtttttaa gctagtttta ctatcttcta tgtttatata ataactcttg
    16021 tgtgtgaatt taattgataa ttaatatgaa aattatgtga agttttgata ttaggttata
    16081 ctttgaactt tatctaacat tttaaatttt atatctctaa attatctaaa attactatat
    16141 caattaactt tacatgataa aaaggctgtg tataaattta tttttaaaaa ttttactcac
    16201 agccttaaat taaattttct atttaaagta tatataaatt ttattaaata atttgctttt
    16261 tatttcttat ttcataaatc ctagtattct tgataacatt aatgcaattg tagtaggtac
    16321 tttccctgct tcaaatgtag ctgttgctgt gtttaatgct gttactgcat catctatttc
    16381 tttttgtgtt gcatctgctt tatctaatat tgcttgtgct tcatctattg ctgttttata
    16441 tgtcgctttt gatcctac
//
LOCUS       DAMKPG010000081        16389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMKPG010000081 DAMKPG010000000
VERSION     DAMKPG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.085"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            89..1453
                     /locus_tag="PC621_002781"
     CDS             89..1453
                     /locus_tag="PC621_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF4109928.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1592..2758
                     /locus_tag="PC621_002782"
     CDS             1592..2758
                     /locus_tag="PC621_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF4109929.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2875..3657
                     /locus_tag="PC621_002783"
     CDS             2875..3657
                     /locus_tag="PC621_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF4109930.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3776..4429)
                     /locus_tag="PC621_002784"
     CDS             complement(3776..4429)
                     /locus_tag="PC621_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4109931.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4453..5817)
                     /locus_tag="PC621_002785"
     CDS             complement(4453..5817)
                     /locus_tag="PC621_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF4109932.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            6475..7905
                     /locus_tag="PC621_002786"
     CDS             6475..7905
                     /locus_tag="PC621_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4109933.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            8008..9111
                     /locus_tag="PC621_002787"
     CDS             8008..9111
                     /locus_tag="PC621_002787"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF4109934.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            9126..9362
                     /locus_tag="PC621_002788"
     CDS             9126..9362
                     /locus_tag="PC621_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4109935.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            9379..10428
                     /gene="vorB"
                     /locus_tag="PC621_002789"
     CDS             9379..10428
                     /gene="vorB"
                     /locus_tag="PC621_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF4109936.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            10430..11170
                     /locus_tag="PC621_002790"
     CDS             10430..11170
                     /locus_tag="PC621_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF4109937.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            11173..11712
                     /locus_tag="PC621_002791"
     CDS             11173..11712
                     /locus_tag="PC621_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF4109938.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            11836..13935
                     /locus_tag="PC621_002792"
     CDS             11836..13935
                     /locus_tag="PC621_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF4109939.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(14202..14717)
                     /locus_tag="PC621_002793"
     CDS             complement(14202..14717)
                     /locus_tag="PC621_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF4109940.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(14809..16020)
                     /locus_tag="PC621_002794"
     CDS             complement(14809..16020)
                     /locus_tag="PC621_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF4109941.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         6209 a         2017 c         2791 g         5372 t
ORIGIN      
        1 attatgatat aaaaaataat gttaggataa atttaaatct ttttctaaaa atgtattaat
       61 ataattctta aagaaaggat ttttaacaat ggatataaaa gagttagtta aaatgcaaag
      121 aaaatattat aatacaggta aaacaagaga tatatcgttt cgtatagaac agttgaagaa
      181 acttaagtta gttgtatcac aaaatgaaga aaaaatactt ttagcactaa agaaagattt
      241 aaataaatcg aattttgaag gttttatgac agaggtaggc atgttttata gtgaattaaa
      301 ctttgctata aagaatataa gaaaatggag caaaataaaa agagttaaat caagtatggt
      361 taattttcca tctatatcca aagttgttcc acaaccttat ggtgttactc tcataatgtc
      421 accttggaat tatccctttc aattagcact tataccatta gtttggtcat tagcagcagg
      481 gaattgtgtt atattaaaac cttctgaata ttctgtgtca acatctagtg tagttaaaga
      541 catagttgaa aatacttttt caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga
      601 aagtgaaaag ttactattgg aaagatttga ttatatattt tttacaggaa gtactaatgt
      661 tggtaaaata atcatgaaat cagcatcaga gcatttaact cctattactt tagaacttgg
      721 aggaaaaagt ccttgtatca ttttaaaaga tgctaatata gaccttactg ccaaaagact
      781 tacttgggga aaactaataa atgcaggaca aacctgcgtg gctccagatt atgttttagt
      841 gcatgaagat agaaaaaatg agttaataga gaaaataaaa tattatacta ataagtattt
      901 tggagataat ccatgtaata atgagcaatt tcctaaaatc ataaatcaga gacattttaa
      961 tcggatatta tctcttattg ataaagataa gatagtctat ggtggaaatt ataataagga
     1021 gacattaaag atagagccaa ctatagtaga caatgttaat tgggatgata atataatgaa
     1081 ggaagaaata tttgggccta tatttcccat tttaacatat aaagatttag atgaagtaat
     1141 acaaaaaatt atacaaatgc caaatccatt ggcactttat atttttacaa aaaataagta
     1201 cctagaaaat aaacttttag aaatgattcc agcaggcgga tgttgtatca atgatacagt
     1261 tactcatata gctacgaatt acttaccttt tggtggtatt ggtgaaagtg gtatgggctc
     1321 atatcatgga aaagctggat tcgatacatt tactcactat aagagtgtgt taaaaaaatt
     1381 aaatttggat gtccctatta gatatgctcc atacgataat aaattaatta aggttcttaa
     1441 aaagataatg taggttatat tgtagaataa taaatataaa taaaattgag tatttgtatt
     1501 ctgaatatat tttattttta atttggtata tttgttataa aagtggtgat tttgtgataa
     1561 aatttaatta tatagaaata aggaggaaaa aatgaaaatt aattggaata caaaatacac
     1621 aacaatagca atttatacat ttataatagc tgctagtagt attatatttt atctagtatc
     1681 atcacaaatt gatgtatttt ctaataattt agatgctata tttacaacat tacaaccttt
     1741 tataatagga tttgctattg catatcttct taattttatt ttaaagtttt atgaagacag
     1801 aatttttata aaaagtgaga aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg
     1861 tcttttatta acatatgcaa cagctacatt gatactttac ttatttatgc atttcgtatt
     1921 gccacaagta attgagagta ttgttggact agcaaatgat atacctatgt atgtaaacaa
     1981 tgcaactaag cttattgata aattaatgac tgatttaaat cttgatgagc aatactttaa
     2041 tttggcagtt gataagtgga atgagtttgt tacatatatt ataaagtttg taacagattt
     2101 aatcccaata ttaggaagta tgttaaagaa tgtagcctct agtatatgga atattgtact
     2161 aggattaatt gtttcagtat acttattaat tgataaagaa aaattctatg gattaagcaa
     2221 aaaaataact tatgcagtat ttacagaaaa acaagctgcc agaattttag aattaactca
     2281 tagaagtaac tatacatttg gaagattttt aggtggcaaa atattagatt cattcattat
     2341 aggtattcta acatttgtaa tacttacatt agttaaaatg ccatatacat tattaatatc
     2401 agttataatt ggtattacaa atattatacc tttctttgga cctttatttg gagctatacc
     2461 atctactcta atagtgatgt ttgtatctcc tataaaagct ttctggttat tgttgattat
     2521 attaataata caacagatag atggaaatat tataggacct aaaatacttg gagattcaat
     2581 tggtatatct gcattttgga ttttgttctc cttattggtt gcaggaaaat tattaggttt
     2641 tattggaatg gttataggag ttccaatgtt tgcagtaatt tattctataa ttaaggatat
     2701 tgttgaaagt aaattggaca aaaagggatt accgacagat acttccgatt acatgtagct
     2761 aaaaaatatt atgtataatt aaaaatatat aaattaagca atatttaaag cactgagaaa
     2821 gttaaactct tggtgctttt tagatataaa attattatag aagaggtgtt ttttgtggca
     2881 tttatagaat gtaaatttaa atctaaatat actggaggag aaactgatat aattgtaatt
     2941 ttgcctgtag caaatggaaa agaacttttt agtggtaaag agatatatga ttttgaaggt
     3001 aaatttaaaa cattatactt atttcatggt ttagaagatg accaaagctc atggattaga
     3061 tatactaatg tagagcatta tgctaaaaat aaaaatatag ccctagtaat accaagagta
     3121 gaaactagtt tttatacaga tatggcgtat ggacaccaat attttacttt tgtaagcgag
     3181 gaattaccta aatttgttag agctatattt cctttatcag ataaaaggga agataacttt
     3241 gtagctggta tgtctatggg gggatatgga gctttaaaat tagcactttc aaaaccaaaa
     3301 gagttttcgg cagttgcaag tttttctggt tcgcctgata tgattcatga gttagaaaat
     3361 caacaaatag aaaccaatgc agacatttta tttcacgatt ttggtagacc agaagacgct
     3421 agaaatagtg agaatgattt actttacata cttaagaacc ttaaagaaag aaatgaagat
     3481 attcctaaaa tttatcaatt ttgtggtgat agagattttt tatataaaaa taatcagatg
     3541 tttaaaaaat atgcagagtc tctgggtgta gatgttgaat atgaagagtg tgtagggggg
     3601 catgaatggc gtctttggaa tttatggata aagaaattta tagatataat tgggtagtac
     3661 acttatggta ttaacaggta tactataaaa tatctttaaa tatgataaag actgaaatcc
     3721 taaaatcaaa ttatgatttt aaaatttcag tctataagaa tttaataaaa ttttattaag
     3781 ttcttacacg agttattatt ggagtaaaat tgtcaactat atctatatag tatttactca
     3841 attcctctaa aatctcttcg cataaatccg tactatgacc aagattaata agaaactgtt
     3901 ttgtaaaatg ctttattaca gcttgagaag atatatcgta cttgtgtgaa gtataaactc
     3961 taattatttc aggtttcata gcaagtatac caatacaaga aagtattaat tcatcatgtt
     4021 ctatagattc tttatcatta acatatcttt tcatgaccat ttcggttaca cttctttgat
     4081 agcttaaaat agcttcttca taagctatat ccaaataaaa atttctatat gcttcgtttt
     4141 ttagaaggaa acaaaggtca gcattcatag caagaaagaa catcatcata gtaccttctt
     4201 catggtagtt atagattaaa gactgcattt catttcttat atcttcatat attatagctc
     4261 ctatttcgct tttacttgag aagtaatagc ttattagacc taaatttata ttagctcttt
     4321 ctgcaatttg cctagttgtt gtcttatgat aaccttgctc aaaaaataat tctctagcag
     4381 cacttataat actagctttt gtttctttgc ccttttctga ttttgacata tttactgtgt
     4441 atacctctct tttcaatctt tttttgaaaa tttgctaact ataaatattg taatagaacc
     4501 acctattata ggaaccaaaa ttctgtcaaa tacagtaaga ataccaatca tatttaataa
     4561 tgccaatata acacttacta taacagcata gatagctcct ttttttgtag cgcctttcca
     4621 gcaaagacca attacaaatg gtgcaaatat tacacctgta cataatggag ccggccaaaa
     4681 caataattct aataaactat caaatttaag tgctatgatt gcacctatta tagctgtcaa
     4741 agctgatgca atttgagaat actttagagt attttttaaa tcattaaatt ctatattagg
     4801 atgtaataat ttgttataaa tatctcttga tataatagca ctcattgagg tcattaatga
     4861 atcggcagta gacattatgg cagccaatat agcagctaag aataatacag atattaaagg
     4921 tggcaaagta gtcgatatca tttgaggtaa aacctgaaca gatgatatgt ttggaaataa
     4981 actaccacca atagctccaa ttattcctat agggaaggca agaaataata gtataaatcc
     5041 tgcaattaca ctacttttaa ctgcattttt agcattatca gcacattgat atcttaaaaa
     5101 actttcataa gaaaatattg agcctaaaac taatggtaaa actgtataca taaaatcact
     5161 aatcgattta tttcctggaa agaatggttc ttttaccatt gattgtaaca taagtaaagg
     5221 gttgtcgcca actaatttta agcttatata agctactatt ggcaaaaaca ctatacaaat
     5281 agcaagttga ataaatgctg atactgcaac tccccacatt cctgatgtat aagtatatat
     5341 aattataact ataactccta taattacagc tatatcataa ggaataccaa atgcagtaga
     5401 tacagaacca aaagaaatta attgactacc cataatagct agtatagata ttggtgttac
     5461 aagactttga taagctctta aaaatacact atgaccatat ctagtttcaa tataatcagc
     5521 aggtgttata tcacaagata agcttcttaa ttttcctgcg aaaaaggcat ttataaagca
     5581 agctaaagct gcacctatag tagaatacat tccaacccaa agtcctcttt cagctcccca
     5641 ttgaactcca ccaactaagc accctcctcc aaaagatgta gcagcaatgg ttgcagctag
     5701 tataggcaaa ggaagatttc tacctgcgat taggaaatcg gatgctgttt taactttttt
     5761 tgaaaaataa aaaccaatcc atatgtatat tgcacacgta acaattaaaa gtaacatagt
     5821 cttttctcct ttataaaata ttttaataaa atatataaaa tatatttaaa atatatttta
     5881 tataaaagta gtacgatatt tgttttattg gttttagatt aatatagcta taataattta
     5941 tgatattaga tggaaattac gtaaaaatag aaaattgaaa attctgaaat ttttgcatat
     6001 tgatatttat ctcatagatt cttttttgta attgtaacac gcttttattt ttaaatcaat
     6061 agtacatgta taaaaataaa aggaatctaa ataagaaaaa gatattttta atatctggat
     6121 gtagtataga aatatcttga ataaattatt cttgaataaa attataaaaa tttaaaatca
     6181 ctagaaatat gataaaataa tttaaaatac gcatttcaat gacatttcaa gacaagaaaa
     6241 taataaaaaa caaatctaaa ttaagcctaa atagcttttt agttaatttt agtagtaaaa
     6301 aataaaactt aaaaagtgtt tgacaattat gaattttaag aatataatta aattaaaatt
     6361 atacacatat aaaaatatac tcctgaatca agaatgagat atttaagatt caaaatatat
     6421 attattatac gtgaataatg aaaagtaaga aaattttata agggaggggt agatgtgagt
     6481 agtcaagaaa gcaaaccaaa aggaaatctt atatcaaatt ttagagatga tatgagagac
     6541 cctaagaaaa gagcaatata cacatattca agttctataa acttagcgta caattttata
     6601 ggttactttg tatcaactgt tacaaatatt tttttaactg atgtggcaaa acttggtgta
     6661 attatggcag gtggtatgca aattatacaa tctttcataa aaattgctgt agctcctata
     6721 acaggtacag tatttgacaa acaaccattc aaaaaaggaa agtattatcc atggcttaga
     6781 tatgcaccag cagcgttagc attaacatat ggaatatttt tcatagttcc attaatgaaa
     6841 ataagtccag cattactttt acctttgatg acagctttat tattaatagc atcttgtgct
     6901 cagcaagtag ttttcactat aatttatgct atttaccctg tagttgctaa gacacctaga
     6961 gaccgagtta taggctcaac tactactaat atatttaaag aagttggtaa attcttagta
     7021 ggtttttcat atccgttatt attagtttta tttgcagatg tattaggtgg agaaggaatg
     7081 ggttatttag gtacctactt attctttgca gcaggatgcc ttttagtatt ttggttttct
     7141 tcaacagaga taataaaatc tggagcagaa gaagaagttc ttcaaaaaga agagaagcca
     7201 aagattaaag caagtgaaat ggcaaaagct ctattcacta atgtaccact tatgatggca
     7261 ttttgtttag aatttcttat atgtataaga tctataggat taggaccttt ggctccatat
     7321 tattttaaat atgtaataga agatgaacgt ggattagcag tatttttatc aatcatgcct
     7381 ttggtaagtg tagcattcat gtttttcgca ccagtgttta taaaaatctg tagagagcaa
     7441 aaactagctt caataatttc tttttctata tgtgcactat gccacttatt ggttgcatgg
     7501 actccttggg ggaaaactac tttaggagta actatgttgc tggcaattgg tggaggtttt
     7561 tcaaatgtag tatcaataat aaatcttaat ttctttgccg gttcttgtga ttatgggcat
     7621 tggaaatcag gaaaagactt acctggctta agtatgtcac tatatccagt tgcaatacaa
     7681 gtaggagtat tacttgcaac tactataaga acagttttaa tgaactcaat gggatatcaa
     7741 gctgatatgg tagtcacaga agcagttaaa tcaggtttta taaatatgat ttcttattct
     7801 atggcaatac ctttaataat agctgttgtt atagcaatac tatatccagt aagtgacaag
     7861 aaattaaacc aaataagaga agagttaaat caaagaaatg cttagttaaa agaaagttaa
     7921 gtagatggtt aatttaagct attaaagtaa gtagacgcaa tagcgtttat ataaatcaaa
     7981 atacatatac agagggggct tttaaaaatg atagaaaaat tattgagaaa taccgtaaaa
     8041 gaattacatc aatatgttcc aggtgaacca atagaaaaag ttaaggaaaa atatggtgta
     8101 aaagaaataa taaaattagc ttcaaatgaa aatcctctag gaccatcacc aaaagcaata
     8161 gaagctatga ttgaaatgct aaaacaaggt caactttatc cagagccaga agctaacgaa
     8221 cttagaagaa aattagctga aaagctagat ttaaaaccag aaaattttat tgttgctaat
     8281 ggtgcggata acgttatcac tctaataggt gaggctttta taaatagagg agatgaagtt
     8341 atatattgta acccaacatt tccatcatat agaactgcaa ctataaaaaa tgaagggata
     8401 ccagtagaag tgcctttaac agaagattat aagtatgatt tacaaggtat attagataag
     8461 attacagata aaacaaaatt aatatgtgta tgtaacccta ataatccaac aggaacaatt
     8521 gtggatgata aagagttaga agaattttta aagaaagtcc ctgaaaatat tataactata
     8581 ttggatgaag catatataga atttttaaca gttcctaatt atgtggatgg acttaagtat
     8641 gtaagggaaa attataacgt tatagtaaca agaactttct ctaaaatata tggtcttgca
     8701 gggcttagag taggatatgg tattgctaaa gatgagataa taagaacttt atttacagta
     8761 aaagagcctt tctctgcaaa tcgtgttgct ataagtggag caacagcagc acttgatgat
     8821 gaagagttta taaaagaaac atatgaatta aatagagttg gaatgactta ctttaaagaa
     8881 gagtttacaa aaatggggtt tgatgttgta gattcacaat caaatttcct ttatgtagat
     8941 atgaaaacag atatacctaa gttatttgaa gacttgaaaa aaagagggtt tgtaataaga
     9001 ccaagtgcta cacatgcacg tgtaagtatt ggaactatgg aagaaaataa gaaatttgta
     9061 gcagttttaa aggaaatatt aaatattaaa ggagagccaa gtattggata atagatagaa
     9121 atattatgaa acggggtgta gatatgaact taaaaataaa cgcagagaga tgtaaaagtt
     9181 gtgaatactg cgttatatct tgtaaaaaag gagcattaaa aatatcaagt aagataaata
     9241 aagaaggtta tgctcatgta gaagttgatg agagtaaatg catactttgt ggaatttgct
     9301 atcaggtgtg tccagataat gtttttgaaa taattaaaga aaaagcatgt caagaggctt
     9361 agaaggagtt gggataatat gaaaaagatg tggaagggga accacgccat tgcagaagca
     9421 gccctaagag gaggttgtga attttatgca ggatatccaa ttacaccaca aacagaggta
     9481 atggaatttt tatcacatag aatgtctgaa cttggaagaa cttttataca gtccgaaaat
     9541 gagatggctg ctatatatat ggtatatgga gcatatgcaa gtggaatgag gtctatgacg
     9601 agttcttcag gaccaggaat atctttaaaa caagaaggga tttcttactt atgtgcaaat
     9661 cattaccctt gtgtaatcgt aaatgtacag agatgggggc ctggtcttgg ctcattggac
     9721 tcagcacaga cagactatct aagagatact agagggggag gaaatggaga ttatcatttg
     9781 atagtttatg caccaaacag tattcaagag actgtagatt tgatgtataa ctcatttgat
     9841 gtagcggaaa aatacagagt gcctgtcgaa attttgacag aagcagcttt aggtcaaatg
     9901 atggaaccag tagaatttcc agaatttaag aagagagaag gagatttagg ttggacttat
     9961 gatggaagta atagagacca tgcaaaggtt gcagataacc aaaagccaac tttttgtatg
    10021 gagaaacgta tgagaataac tgaaaatgaa caacaatggg aagattatca agtagaagat
    10081 gcagaatatg tgtttgtagc gttcggtata ccatcaagaa caactatgaa tgctgttaga
    10141 agacttagag aacaaggtga aaaagttgga ataataagac caataacagt atggcctttc
    10201 ccatataaag catttgaaaa ggtaagtaaa gatgtaaaag gatttatctc agtagaatca
    10261 acagacacag gtcaattagt agaagatgtt gctttagcct caaagaaagt atgtaaagaa
    10321 aatgtacctg tgtatggttt atttagtgga aatcatatac caaaaacatt gcaagtaatg
    10381 gacacttata gcaaaataaa atctggcgaa atcaaggagg tgttctaata tggctacaga
    10441 aaaattaaca cctagcgttg tttcaatacc aaacaagttt tgcccaggtt gtggtcatgg
    10501 aatagtaaat cgtataatag cagaggtaat agaagaacat ggatatgaaa aaaatcatgt
    10561 attaacatta ggagttggtt gtgtctgcaa tatgaatttt agctggaatg gtgataagat
    10621 gcaaactgca catggtagag cttcatctac agctatagga gttaaagtag cacttccaga
    10681 tacattagta atgacttacc aaggtgatgg agatgcttat gttataggtc tttctgaaac
    10741 tttaaataca gcatatagaa atcataatgt gactgtattt gtaataaaca ataataattt
    10801 tgcaatgact ggtggacaaa tgtcatggac cactatgccg ggtcaagtca caacaacaag
    10861 tgtaaatgga agagatataa agactactgg tagtccactg aaagttcctg agatggtggc
    10921 aaacttcttt gatgtagcat atgtagcaag aggttcagtt catagcccaa aggaaataat
    10981 taatctaaaa aaatatataa aaaatgcaat agaagctcaa ttaaatggag agggatattc
    11041 cttagttgaa atattagcac cttgcccaac taactggggt gtatcactag agaaatctat
    11101 caaatggatg gaagaagaaa tagtgccata ttatgctctt ggcgaattta agcaaaggga
    11161 tggtgaatag ttatggaaaa agagctagta tttgcagggt ttggtggtca aggagtttta
    11221 actcttggtc tgattgtagc tgagtcagca cttgaattgg ggaaacaagt aacttggatg
    11281 cctgcatatg gtccaactat gagaggtgga aaagcctata gtgtagtaaa gttttctgat
    11341 gagagtatag gtgggcctga tatggaagaa atagatgttt tagtagcaat gaataaacca
    11401 tctttagatt atataaattt agttaaagaa ggtggaacag tagttattaa tacatctgca
    11461 atagatgaga atgtaacatt aagagaagat ataagtgtag ttaaaataaa ttgtcaggaa
    11521 ttagctcaaa aagtaaacaa tccaaaagca gcaaatatag tagtacttgg agcattaatt
    11581 gctaaaactg ggttacttga aaaagaatta gctttaaaaa ctatgtgtga tttctttgag
    11641 gaaaaaggaa aaggaaagtt taatgttcaa aatgaagcag cattcatgga aggctataat
    11701 aacgccattt gataaattac ccttatctta agtgatttat ctcaagattt aataatcttt
    11761 agtgccaaga gaaacttcat cttgagataa aattcactta aaatcacaac tataaacata
    11821 taggagcgtg aatttatgag tttggaaaaa cttattagac caaaaagtat tgctatagta
    11881 ggagtgactg ataaacttgg ttttggaaga agtgcagcac ttagtatagt aaaaagtaag
    11941 gaaacagata gagtatatta tgttaatccc aaaagagaag aattgtttgg gagaaaatgt
    12001 tataagatga tacaagaagt tccagaagta gtagactgtg tagtagtatg tacgcctaga
    12061 aatgtagttc catctgtatt aaaagattct ggagagttag gaacaaaggc agcagtggta
    12121 tatgctagtg gatttgcaga ggaaggtaca gaggaaggta cagatttaga aaatcaactt
    12181 atagaaatat caaatacata cgatatgaaa atattgggtc caaactgcat ggggttgcta
    12241 aattgtatag ataaggttaa tctatgggca ggaggttcta aatgggcttt agatactaaa
    12301 aaacctggta taggaattgt aggtcaaagt ggatttataa ctgctgaaat tgtttcaagt
    12361 gattatttta atatatctta tggtttttca acaggaaatg gaaatatagt tacactagaa
    12421 gatgcagtag atttccttgt agatgatgat tatgcatcag taatagcaat ttaccttgaa
    12481 ggtttaaaaa atcctcaaaa atttatagat gctttaaaaa gggcagcaca aaaaagaaag
    12541 ccagtaataa tattgaaatc aggaaggtca gaaaaaggtg ctatttctgc tgcctctcat
    12601 acaggaaatt tagctggttc gagtaaagct tttgaaagta tttttgaaaa atatggagta
    12661 atttctgtgg aaaatttaga acagtttatg tgtttagcac aagcttttag tgttttagat
    12721 ggtaatttac ctgcaaattc taattttgca gcaataaact tctcaggagg agaaaatact
    12781 atttgtgcag atttagcaga agaaaatggt gtagaacttg cagaaatatc ttctgaaact
    12841 aaagaagaaa tgaaaaaata tcttccagga tttgcaacac ctaaaaatcc actagatgct
    12901 acaacagcat tattccatga aaaggatatg atagtaggtt tattacatac ctttgaaaag
    12961 gatgatagtg ttggatttac tatggtaggt gcaaatataa gagatgaaga aaatgaaatg
    13021 catgaaaccc tttgtaaagc tgtaagtgaa gctagagaac aaggattgaa aaaacctgtg
    13081 tttgcagttc ctacacttga aggaaataga tatttagatt ataggactag attagaagat
    13141 aatcaagtgc ctataatgtc atcagttgga actacattta cttgttttaa taaaatggct
    13201 aagtttatag attatgatta ttcaaaaaga actttagaat ttaaagctgt taagaaaaga
    13261 gagtcaaata atgtagtagc attatcagaa ttagatagta agattgagat gaaaaaatat
    13321 ggagtaccag ttccaggaca aggtaataca aaaagtattg atgaattaga tgaaatctta
    13381 aaatatatta agtatcctgt tgttttaaaa ataaattctt ctgaaatatt gcacaaatct
    13441 gatgttggag gagttaaaat aggaattaag aatagagatg aggcagtaga tgcatataat
    13501 gagattttaa ctaatgtaaa aaaagcaaaa ccagatgcta atattgatgg tatattagtt
    13561 caagaaatgg tagaaagtgg tatagaaatt attattggta taactaatga tgaccaattt
    13621 ggaccaatgt tattagtcgg tttaggagga gtatttgtag aggtatttaa agatactaca
    13681 ttatatccat tacctataaa tcatgatgaa gctattatga tgcttaaaaa actaaaatca
    13741 tttaagcttt taaatggcta tagaggaagt gagccttgtg atattgatgc tcttgcagat
    13801 atgatggtca aattaggaaa atatgcttat gaaaataagg atgaagtcaa ggaaatagac
    13861 ttaaatccag tgtttgtata tcctaaagga aaaggcgtat gtgcagtaga tgccttaata
    13921 gttaaatata aatagtttat aagttttggt attcaaccat aattaaagca ccgactgaaa
    13981 atcacatttt gtaagttaga tagtgtgatt ttcagtcacc ccaaatttaa tttaaaaata
    14041 ttttaaaatg aaagtatagt tttgagttta attgtgaaat agcactgtat aaacaataaa
    14101 atactacgta taaaacgaat aataaaaggt gttttaatga taagaatcaa tcttgatttt
    14161 cattaaaaca ccttctttat tatattaaaa atttttaaac gttatattgt cacaggtggg
    14221 tttgttggag ctgcatatgt acgattagca aattcttggt caacaaagaa tagaccaccc
    14281 tctccagcaa gtttaacacg ttttatattg tcttcacaat ttgtttcttc ttctgcttgt
    14341 tcagatataa accaaagtaa gaattgagaa gttctaaagt ctgattcaga gtttgctaca
    14401 gctgctaagt tgtttattaa agctgtaacc tttttttcat gagctaacgt acgctcaaga
    14461 atatccattg cagaagtaaa gtctgaatct ggcattggga tagcatcaag ctgaaccttt
    14521 cctcctacat tatgtaagta tttgtaaaaa aacatagcgt ggtctgtctc ttctttatat
    14581 tgaacataaa accaattaga aaatccttta agtccttctg cttcaaggta agaagacata
    14641 gaaagataaa gatacgcaga atataattca tggttaatct gctcattaag taatttttcc
    14701 attttagctg aaatcatttt tatacctcca aattttaaat gctcttataa aatataataa
    14761 tattaaatgg aaaaatattc aataaaagtg tttaaatatt ttacatattt aataaaaatt
    14821 tatactacca ttaaaaaaat tataacttac aatggctata gagccaatta atggtgcatt
    14881 tgctccaaaa tttgaatgcc ttacacttat ttttttatat ttagaaaaac ttgctgaagc
    14941 agtcaatttt tttaataatg tattttctat aaatgtacca ggtatattaa agtcatagcc
    15001 tattattata gaactacaat caatcaatgt aagtgtatta actaatgcat atgatacgta
    15061 tgtacaaaat tcatcaagca aaaacatggc aagactatct ttattatttg cctcatctat
    15121 taacttgact aaagaaaaat ctttacagtt aattaatggg gagttaggat aaatatggct
    15181 caatgattca attcttttaa ttagattact gacacttgta taataatcta aacatccatt
    15241 gttaccacaa ctgcacaatg gtccactaaa attaattgat gtatgtccta tttctccact
    15301 ttgtcctagg ttgccagtat gaagtttatt ttctagaact agaccagctc caataccatt
    15361 cattatgtgt aggtatatat aatttgatat atttttacct aaaccataca ttttttctgc
    15421 taatgcaccc gaattagcat catttatcaa aaatattgga aaatcagaca tttcttttat
    15481 aaaatctact atatttaggt ttgatacatt tccaaagtct gggggattta gtatggtacc
    15541 atttatatca tctactggac ctattgaaga aataccaatt ccaattatat ctctcttgtt
    15601 gattttcatt attttagtaa acattctttt tattatatct tttaaaacat catttgatag
    15661 acatttaggg taagtttcag aaagttgttt tacaatatta ccttttaaat ctgaaataat
    15721 taccttacat atatttcttc ttattaggat tccacaaata caaggcgata tattagaaat
    15781 atctaataaa ataggtttac gacctgatga atgtgcattt aaagttgatg actctatttc
    15841 cctaatcata ttttcattaa taagttcagt aactagattt gataaagtca ttttagtaag
    15901 tcctgtagac ttagctaaat caactctgga cataggtgta tttgtagaaa gtaatttcaa
    15961 tattattaac ttatttttaa tttttacatc tttgatgtta gaaccttgtt tgttactcat
    16021 cataaatctc cttaaataaa gttaagtgtt ttatttattt tcaagtatac tatcacctta
    16081 taatagagta acattttttt acttgaaaat atcattaatt agaatatatc acattatata
    16141 tcataaaaca tataaaaaaa tgttagaatt aaattaaaag tgaaaagtag attgatatat
    16201 aaatagtaaa tacttaatta aaaaaagata atgatagcaa taattattgt taccaagaat
    16261 ctactacttc aagtgttacc ttaggtattt aagttgatag tggtttgcac gtaataaact
    16321 tgatgtaata tatattctta ataatatcga taaaagattt acttaaaaat aatatagatt
    16381 atgtaaaca
//
LOCUS       DAMKPG010000082        16290 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMKPG010000082 DAMKPG010000000
VERSION     DAMKPG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.086"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(84..995)
                     /locus_tag="PC621_002795"
     CDS             complement(84..995)
                     /locus_tag="PC621_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF4109942.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
     gene            1106..1573
                     /locus_tag="PC621_002796"
     CDS             1106..1573
                     /locus_tag="PC621_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109943.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            complement(1741..2340)
                     /locus_tag="PC621_002797"
     CDS             complement(1741..2340)
                     /locus_tag="PC621_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109944.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            2586..3182
                     /locus_tag="PC621_002798"
     CDS             2586..3182
                     /locus_tag="PC621_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF4109945.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(3280..4239)
                     /locus_tag="PC621_002799"
     CDS             complement(3280..4239)
                     /locus_tag="PC621_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4109946.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(4273..5004)
                     /locus_tag="PC621_002800"
     CDS             complement(4273..5004)
                     /locus_tag="PC621_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109947.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(4997..5767)
                     /locus_tag="PC621_002801"
     CDS             complement(4997..5767)
                     /locus_tag="PC621_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4109948.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(5852..6562)
                     /locus_tag="PC621_002802"
     CDS             complement(5852..6562)
                     /locus_tag="PC621_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF4109949.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(6742..6894)
                     /locus_tag="PC621_002803"
     CDS             complement(6742..6894)
                     /locus_tag="PC621_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF4109950.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(6920..9076)
                     /gene="feoB"
                     /locus_tag="PC621_002804"
     CDS             complement(6920..9076)
                     /gene="feoB"
                     /locus_tag="PC621_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF4109951.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(9176..9397)
                     /locus_tag="PC621_002805"
     CDS             complement(9176..9397)
                     /locus_tag="PC621_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF4109952.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(9410..9622)
                     /locus_tag="PC621_002806"
     CDS             complement(9410..9622)
                     /locus_tag="PC621_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF4109953.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(10146..10754)
                     /locus_tag="PC621_002807"
     CDS             complement(10146..10754)
                     /locus_tag="PC621_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF4109954.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            complement(10874..11302)
                     /locus_tag="PC621_002808"
     CDS             complement(10874..11302)
                     /locus_tag="PC621_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4109955.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            complement(11321..11581)
                     /locus_tag="PC621_002809"
     CDS             complement(11321..11581)
                     /locus_tag="PC621_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109956.1"
                     /translation="MFKTVFEDKEYVLLCKNYSKSDGVKAIFIYLISMLLLFFQGYLY
                     TTNLSSTILTNSQMILSILILLIGLGFVLLSKEKLNTIGITM"
     gene            complement(11855..12400)
                     /locus_tag="PC621_002810"
     CDS             complement(11855..12400)
                     /locus_tag="PC621_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF4109957.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12518..14341)
                     /locus_tag="PC621_002811"
     CDS             complement(12518..14341)
                     /locus_tag="PC621_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109958.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(14354..16129)
                     /locus_tag="PC621_002812"
     CDS             complement(14354..16129)
                     /locus_tag="PC621_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4109959.1"
                     /translation="MKYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLREKIGVVFQNSSVFGKSILENIVMGNEGISREDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5486 a         2872 c         1652 g         6280 t
ORIGIN      
        1 ttatcctatc tctacatata agtttcccta tatgctgact aatttagcta ctaactcatg
       61 actaaagtca caagtgtgcg tagctatttt ttaatcaaat gtttaagttc tataggttct
      121 attattgtag cattttttcc aaaagttaaa aaatattcag ccacccaatt aagtgtagat
      181 tttggtatat acatatctat gtatccaccc ttagacaaaa ttttcaatcc tcttgccaac
      241 aaaaattctg tttcacaacg ttttactccc tcatctgtca gattaatttt aatatgataa
      301 tctgtaccta cttccatatt cttaagataa tcatgtatag aagataaaac cttatatttc
      361 ttatttgata agttttctat actcaaaatc ttcacaattc tgtctgcacg aaattcctta
      421 acagattctg attttataca gtaagctgga caataccaaa gtccattcat agcatacaaa
      481 ccaataggaa caatggtacg tatactattt ttttgctttg atgaatattc aattttaatt
      541 gtaaattcat tcattactat attaaacaat tccttcaata atggtgttga gcaatgccta
      601 tctggtgtcc aaaaaacaag tttacgtctc atattctcaa tattatattg aatatcattt
      661 ggtatacaat ttaaaaattt ctttaaaaca gaaatagttt cttgctcaaa tggtaaatca
      721 ttataatact ctagtgactg atatgcaaag aatatagatt ttacctcact ctctgaaaat
      781 gtaattggtg gaagtatacg ttctttaagt acatgatatc ctccagccgc tccaactttt
      841 gaatatagtg gaaatccaac actttccaat acctgtaaat ctcttagtat agtacgtttt
      901 gatacagaaa attcttctgc tagctctccc gctgtaaaac ttctttttgc atttattgtt
      961 atcatcagtt caattaaacg ctcagctctt gacatattca tactcctaat ttaaatatag
     1021 tgacaataat tgtcaccatt atatgataag atattcttgc atctaaagca ataataatat
     1081 taaagaaaga agggattgat attttatgga aaatgatact attaaggctg atgatattct
     1141 caattattgt ctatcaaact tagatgatgt tgtactaatg gatagttggg gggaacgagc
     1201 aatttactac aatcctaatg gtgttttaaa gcgaggggta tatgttctta ccattaaaga
     1261 aaaagacagt aatcatgata aaggttcgtt ggttagtcgt ccaaatgtat accgtgtgaa
     1321 tataggatta aaaaaagaaa cttttattga aatgtttgga tatattccaa agcgtccagg
     1381 tgtagggcaa atagttgata tggattttga ttttacaaaa ttggacacaa tcatgccaca
     1441 tcctatctac tcatggatgg gatggatatg cgtcctaagc cctactgaaa agacatttga
     1501 gaactttaaa actttaatag aagaatctta caattttgca aaacaaaaat ttaaaaagag
     1561 aaaaaacaag taaaaacata aaaaaataac ctctaaataa actataatga aaatatgagg
     1621 ttacaagata aaaattagta tcttgataac atacaaaaaa gcgaagccaa aaaatatatt
     1681 aattacaagg cttcgctata tttcaaatat ttatttctaa ctttaaaact atttactcta
     1741 ttatttttta ttatttcctg ttattcccaa tctatcattc atatctttta tcatttttct
     1801 aaaaccacta ccaatatcgt ttccaacatc ttcagttcca tgaactagtt tcataaaatc
     1861 attttgacct tttttcatat cttcatcaga taccttatct aatttttcta ttctctcatc
     1921 tatatctttt actaatcttt ccccttcagc tattaggtca tcattcattc tctttaactc
     1981 tgtatcttct gttttaccgt cttttttaag agatgttaca aatgattgta ttttatctct
     2041 actatcttta tatgcagcct ttacatcact tagatatttt tcatttcctg gatattcttt
     2101 atctttaaac tgattgttta tactttctgg agtatcataa atagaatatc tatttagacc
     2161 tgctatataa tcattataac ttgttgaata ataattcata tatccttcat tattatttac
     2221 agattcatta ggattttttc ctgcattttc atttggtttt tcattatccc tagcacatgc
     2281 agttattcct atagctaata ctaatgctaa tcctacaaat aaagttcttt ttaatttcat
     2341 ttttattacc ctcctagatg ttttgtacta caaaaaattt taataatttt ctttcttaat
     2401 ttagtatttc tatattttta ttttttattt actaaaatta aacaagttat aatttatttt
     2461 tttggaaatc ataatgaata aataattaaa taaaaaaatt ataattttat tattgatatt
     2521 gattatcatt catagtataa tctaattact tgatattaat tttcatttga aggaggttat
     2581 cttttatgtc ttgttgtaat tcacaatttt gttgtaaaaa taaaattgaa tcatcatata
     2641 taaaatggct tttagaggtt ttaggtccag tctttttagg ctctaaacct gctgaaataa
     2701 taaatatttc gtttactgat gtaaatagag aggaaaaaat taatgatatt tataaatatc
     2761 tatataaatg taagaaaata gattttatag ttattgataa agaaaaaaag ggattaaaaa
     2821 tcttatttgt aaataaaaaa gcattatcag aaaaattaaa atgtaaaaaa acagtaaact
     2881 ttttaaagtt tttaggatac aaacaaaata cgaatgttaa tgcttattta gagcatttag
     2941 taaataaact taagagtgat gtttttcctg atgaaatagg tattttttta ggatatcctc
     3001 ttaaagatgt gattggattt atgggatata gtaattatga agtttctatg attaaatatt
     3061 ggaaagttta tggtgacaca aaacaatccg aggacacata ttcaaagttt ttacttcacc
     3121 gtaagaaaat gagaaaatta ctagactaca taagtgtaga taaaatagta agttgttttt
     3181 aattgtattt ttgtataata ataaaaatac accctctttt accatacagt ttccataata
     3241 aataagggtg tattttccac tttaatattt taaagtattc taattttgat tgtacaacat
     3301 atctttaata tttattttct tttgttgcat tccattagac atcaaaaatt cctgtgtatc
     3361 ttctaattct tttatatcct tgtccgttat tttagaacta aagtcatacc aagaatacat
     3421 ttcttttgtt tcatctaaag ataaaccaac ttctttagat acaacatcca tcacttcatc
     3481 cttgttttca ttcatatatt ttaaagtttc ttcatgaacc ttcataaatc tttttactaa
     3541 ttcaggatat ttttcagcaa agtcatcact tacagcagta actattattc cacttactaa
     3601 tccttctcca tttacaatca ttttggcacc tgaatttatc gttttcaatg ccactggacc
     3661 tgcaagcatt gctacatcag cacttttact ttctaatgca gaagcagcac taggaatatc
     3721 catatttaca tattcaacat catctatagt atagccttct ttagcaagtg cagcaatcaa
     3781 cagttgatga agtatagtcc cttttggacc tacaattttt ttacctttta agtcttttat
     3841 agatttaata ttatcattat tagttataag catatatcct tttggtgaac ggctataaat
     3901 attcgtaatt ttaagaccaa caccatttga tgcagctatt attgcagacg ttcctcctaa
     3961 tgcatgtaaa aagtctagct cacctgctgc taatgcttga gtttgctctg gtcctgttgt
     4021 caattcatgg aagttgactt ttatattgtc ctttttaaat tcctttccaa ataaatcatc
     4081 ctttttttct aatatactag gaacattaag tggtgatttt acataagtta aatttatttc
     4141 cttaggtaaa ctagcctctt tttttgcagg ttcatctttc ttatcatttg ttgttgaaca
     4201 tcctacagtc ccaaccaaca ccattattaa agatgtcatt actgctaaaa tctttttgtt
     4261 tcttttaatc aattaaaact cccccaattc ccttaatatt tcttttttca agtttatata
     4321 atagttttta gttaaatctc tattatattc atcttcgaca ctaaattgtt taatcctttt
     4381 atttttacta aaaacaatta ttttctttgc tatttccata gcttcttcaa tattgtgagt
     4441 tacaaatact acaccttttt tagtattttt atggatattt acaacttctt tttgcatttt
     4501 tcttcttgtg aagtaatcta gcgctgcaaa tggttcgtcc atcagcaaaa tatcgggatt
     4561 gaaggaaagt gctcttgcta tacttactct gtgagccatt cctcctgata attcatttgg
     4621 atatgagttt ttaaactttt caagcttcat catttttaaa tacttgtcta aatcaacttt
     4681 atttctatta tctttttcag tatgtaaaag tatattttca cttacattca gccatggcat
     4741 aagtctactt tcttgaaata ccattcctac ttttggagta cactttttat cattattaaa
     4801 aaaatatatg tttccactag tagcattttc aagccctgct aaaattctaa gtagcgttgt
     4861 tttcccacat ccactttcac ctaatataac agttatctct tcactagata tatttaaaga
     4921 tatattatca agaactaaat gctctttatt atcaactaaa tatttcttat atatattctc
     4981 tagcaaaaat ccacttctaa ccatttaaat caacctgctt ccctttgcta aattttgtta
     5041 ttatctttga aaatagataa tctgaaagta tacctaaaaa tccaataaca aaaattccta
     5101 ccaatacaac atcagttcta gacatatctt ttccatcaga aatcaaatat cctagtcctg
     5161 aagaagctgc aataagctct gctccaatta tagccctaaa actataccca agccctaaac
     5221 taagtccaac aacaatatcc agcatagaat tagggattat tattttataa aaaattctct
     5281 ttttagataa attaaaagat ttacctactt ctattaattt attgtcacaa ttttttatac
     5341 ctttcaatgt atttaaaaat attggaaaaa aggatgccaa aataataatt ataattttag
     5401 attcttcacc tataccaaac cataaaataa gcattggcac caaagctagt ggaggtgtac
     5461 ttcgcataaa ttcaaatagt ggtttgaaat attcatacat ttttttattt actccaaata
     5521 caactccaag tggtatagcc atcaatgaac ttattaaaaa acctattaat atccttctta
     5581 cactggcata aacatttaag aaaatagaac cgtcatatat catttttata aaagtatcta
     5641 agactatttc aggaggaggc agaatatagc tactccatat acccaaaaca catactatct
     5701 tccataaaat taaaattatt aaaaaaatat aatatccttt aaacctttta tgaattttct
     5761 caaacatata aaactccttt taacatatct atccctgcta ccctttttat tttttaatat
     5821 cttaaataat aattaagcta tatcataaga tttaatctat gccaagtctt aatccacctg
     5881 aactcattct atcagaaggt gtagaatctg tttttaagcc agacaatctt tcagatggtt
     5941 gtataatgac tatttgtcca ggttcaacaa attccatatg ataagattct cctgaagtct
     6001 gaccaataaa agtcttccat gatagttttg atactttagg atatggttct cttccagtcc
     6061 aagctactaa tgcatctggg tcaacacaac aaggtccttc aagaattaga ggattaccat
     6121 ctgttattat agcaacatct tgattttgac ctgttttatt tactaaatgt gtagtaaaaa
     6181 gacctttttg agatattacc cctgaaccaa tcaattttac actgtaattt aattgttcag
     6241 aaaatgccaa taagttttca ctctcaacat aaatggaatc acctgggtca attgatatta
     6301 catctacatg gtaagcattt tgagctaggt aaatttcacc ttcaccttca accaccatta
     6361 ttggcatttg ttctcctgtc aatgacctct gaacatgacc taataatgct cttcccaatc
     6421 caccgccatt acttggacct aacagcaatt tttcaaaatt aaaatttcct ttaaaggcta
     6481 ccatagcacc tttttttgca aaaaatttac cacttcccct tgctatacac ataagctcgt
     6541 ttgtaacatc aaatttaaac ataatattca cttatattgc ttaatttagc aataatccct
     6601 cctaatcata tatttatata taattataca gtaaaattac agcatagtct aacctacttc
     6661 aaatatgaaa tttgttattt atataaaaaa taagaacctg ataataaagc aatttaactt
     6721 tttatcaggc tcttatccta tttatctctt agcgctatga caactcttac tacttgagca
     6781 tccagaacaa ccacatccac aactgctacc attctttttt gaatttttaa ccatatacat
     6841 tacagctagt gccattaata ctactacaat agctgctata ataaatgtag ccatattcat
     6901 cactccttat ttatatgaat taatttttca tataacttaa tttacccatt ttttcttttt
     6961 tcatattttt atttgaataa gttaaaacta caaagactac tgctactgct ataaatatag
     7021 atattattgc cccagcaata cttcctccag tacctaaaac taaacttcca acttgattta
     7081 taagaagagc aattatatac gctgttaaag tttggaatcc taatgtaatc catgtccatt
     7141 tccatgaacc catttctctt tttatggctc caatagctgc aaaacaaggt gcacaaagca
     7201 tattaaatgc cataaatgca aacgcactag ctactgtaaa catacttgca actgaagcaa
     7261 gaagtgttgg gtcttgctct gttgcatctg atataccaaa taaaactcca aatgttccaa
     7321 ctacattttc cttagctact agacctgtaa ctgttgctac tgatgattgc caatttccaa
     7381 atccaagagg agcaaatatt ggtgcaacta tatttcctaa gctagctaat atactatctc
     7441 cagcatctac catttgtaat gaccaattaa atgattgtaa gaaccagatt actccacaag
     7501 caacaaatat aattgttcct gctttgatta taaatgcttt tcctctatcc cacatatgta
     7561 ttaaaacacc ttttgcactt ggaacatgat attgaggtaa ttccataaca aatggagatg
     7621 gttctcctgc aaataaacta gttttcttta atataattcc acaaatgatt atcattgcaa
     7681 tacctaagaa atacattgat ggagctaccc atgaagcacc tccaaataat gcccctgcaa
     7741 ataatgctat tattggaatc ttagctccac atggaatgaa tgtagttaac ataatagtca
     7801 tttttctatc tctatcattc tctatagtac gagttgacat aactcctgga actccacaac
     7861 ctgaacttat aagcattggt ataaatgatt ttcctgaaag accaaactta cggaaaattc
     7921 tatccattat aaaagctaca cgtgacatat acccacaatc ttctaatatt gacaataata
     7981 agaataaaag catgatttgt ggtacaaatc ctagtactgc tccaactcca ccaattagac
     8041 catcaactac aagtccttgt aaccactctg ctacatttaa agaagctaga aaattactta
     8101 cattgccttg aataatttct ccaaacagta catcatttgt ccaatcagtt gctatagtac
     8161 ccaatgaact tactgctata tagtaaactc cccacattat aagtgcaaaa ataggtaatg
     8221 ctagtatacg atttgttaca atcttatcta tcttatctga tacagtttcc ttaccttttc
     8281 gattcttctt tattatactt gaaattattg aactaataaa ttcataacga ttggctgtta
     8341 ttatactttc actatcatca tccaattcat cttcacaatt tctagttatt tcttcaatac
     8401 tattcaatat tgttttagaa atatcaagtt tttgtataac attttcatct ctctcaaata
     8461 gctttatagc taaccagcgt gtttctacat ctatatatgg agtcttttct tctattattt
     8521 tttcaatttt ttctattgcg tccttacttt catcaacaaa aggtaatttg aaatttaatt
     8581 tattgttact acttgcaagt tcaactgcct tttcagcagc ctccataatt ccttgacctt
     8641 taactgctgt tacttctacc actgggcaac ctataacttc agatagttct ttaatattta
     8701 tcttatcacc attcttattt acaatatcca tcatattaag agctataatg gtcggtatac
     8761 ctagctctaa aacttgagtt gtaagatata aatttctctc tatatttgat gcgtctacaa
     8821 tatttattac tgcatccggc ttatcatcta gcataaaatt acgtgtaact acttcttcta
     8881 atgtatatgg agataaagaa taaatacctg gtaaatctac tatttccaca tctttatttc
     8941 cttttaattt tccacctttt ttttcaactg ttactcctgg ccagttacca acatattgcg
     9001 acgagcctgt aagcccattg aacattgttg tcttaccaca gttaggattt cctatgagac
     9061 ctatttttat tgacatttcc attccctcca ttattattta aatacatcac tgtttgtgat
     9121 acagaaaatc attctcatta tataattaca ttaaccctac tctaattaat ctttattatt
     9181 ccacaataat tttttctgca tctgacttac gaattgatag ttcataatct ctgactgtta
     9241 cctcaattgg atcaccaaga ggagctactt ttcttataaa tatattaaca cctcttgtaa
     9301 tccccatatc cattatacgt cttcgtgtag ctccttctcc ttctaacttt ttgactttta
     9361 cagtctctcc acacttaata tccttcagtc tgttcatgtc attacctccc tatattatta
     9421 ttctacttgc cattttttta tctaatgcaa ctctagtatc ttttacgtct attattaagt
     9481 taccatctat cttagaaact attttaacat ttgtacctac tacaaaacct atactattta
     9541 aaaaagtcct cgtcttatca cttcccgaaa ttttctttat aactacttct tcacctgtat
     9601 ttgataatat caatggcatc atataatatt cctcctcttc aaccaattta aatttacaaa
     9661 caacctctat cttatatata aatcacaatt tcttaaattt tgactcgtta aaataattac
     9721 ttatcttttt ccttattctt acaattccta attttgattt aagtctatca caatagcata
     9781 ttttttgcaa gtgattattg tattcttttg attttttttc tcatttgatt tattgtatta
     9841 ataaaatcta taaaaattca tactgctatc ctatatattt tgagatttct cttagatact
     9901 cataaccata ttctgcataa gaatgttctt ttgataaaga tattattcta tagtaatgat
     9961 ttttttcttt tcaatgtaat ttaaatatta atctaataca atcataaaaa cataattaat
    10021 tctatgattc tactgactat ctctatctca aaaaatataa tgacactact aatatttttt
    10081 aacattagta gtgtcattat gtttttcaaa ttgattaaat tttctatatt ttataataaa
    10141 acatattagc ctaaacattt agatagaaag tttttttctt taatattaaa aacttctgtt
    10201 tgagcattag aatagttttt tgagtatgta tcaataaaac catgatttgc gtctaaaatt
    10261 tcaatttgaa tattttgcct tctagaaagt tgagaagaaa cataatcaac atcgaatgaa
    10321 tcgtattttg caaacactag caaaatagga catgttggaa ttacatccat ataatctcta
    10381 attcgggagc cataacaaca aattatacca tcacacaaag agttttcact acatctccat
    10441 gcaatagttg cacctatact atacccaact acaaatacat gtttatactc cgattttaaa
    10501 ccatttataa gtttttctac ttttttataa atatcaaact taacattatt tataaaaaaa
    10561 tcatatgctt cttgagcttt taagtatgaa aaaactcttt tttctcctaa gagttctgga
    10621 caaaacacat catacccacc tttataatac ttttgacaaa catcttttat aaaattatta
    10681 actccataaa tttcatgcag tacaacaata gctatattac ttttatgtat atacttaatc
    10741 agattgtcca ccacctgtcg atatttttta catctagttt aaattatttt aagtatgtca
    10801 atttactagc tctaaaattt gacttataaa ataatccgtt tcttacgata aactttctgc
    10861 aaagtaaaat tgactaagta gtaaaaatca gcactcctgt gccaatgtta tacaagatat
    10921 gcagtagcca tgccccccac agacagttag tttttcgata tactaaatca catgaaaagt
    10981 taagtaatat aaggcatatc acataaaatc cagatagaat tgcaaatgaa aaatctccac
    11041 ttgaaatcca ttttacaggg taatgtgtag atagaaaaag aaaagcatta atacaagatg
    11101 aaagtacact attttttatt aatccagtta accttgtttg aatataccct ctaaaaacaa
    11161 tttcttctga aatagcagaa acaataaatg taacaatcat aaaaacagta ggtaattgaa
    11221 aatcaacttt ttttccttgc ataattccag ctgttaaaat aacgaaaatt acgagtacag
    11281 tagcaccaat aactccatac aaaagacttt tatttaaatt tcacattgta atcccaatcg
    11341 tatttagttt ttccttcgaa agtagtacaa atcccagccc tataagtaaa atcaagattg
    11401 acaatatcat ttgtgagtta gttaaaattg ttgaacttaa atttgttgta tacaaataac
    11461 cttggaaaaa caatagtaac atagatatca aataaataaa tattgctttt actccatcac
    11521 ttttagaata attcttacat aataaaacat attctttatc ttcaaataca gttttaaaca
    11581 ctataaatac tcctttctaa ttaaattctt atttatttct catattttat cataaatata
    11641 tgtttaaatc aaattaattc tatttattac ataaatcaga gataataaaa ctaaaaaatt
    11701 aatttcatta aaactatatt tataatgatt taaatattta caagattata gctataaaaa
    11761 atataaaata caactattaa aaaggagcat atcattataa ttttaaaaat cataaatgat
    11821 atactcctaa aaatctactt aacttatatt aaatttattt tccaaaatat ctatttaata
    11881 atcctaagaa tgctttacca tgtctagctt catctttaca catttcatgt actgtatcgt
    11941 gtatagcatc tagtcctaat tctttagctt ttttagctaa ttttaatttt ccatcagttg
    12001 caccatactc agcatcaact ctaactctta agttttcttt tgtatctgca actacaactt
    12061 ctccaagaag ttctgcaaat ttagcagcat gttcagcttc ttcaagagct attctcttat
    12121 aagcttctcc tacttctgga taaccttctc tatcagcttg tctactcatt gctaaataca
    12181 ttcctacttc tgtacactca ccaacaaagt tagctcttaa tccttcgatt atttcttcat
    12241 ctacaccttg agctactcct attctgtgct catcagccca atttttttcg cctttcattt
    12301 cttcaaattt atcagctcca actttacata ctggacattg tgctggtgca gcatctcctt
    12361 catgtatata tccacatact gtacaaacaa atttcttcat aattactttc ctcctaaatt
    12421 ttatatatca aattaaattt tttatttatt aagttagtct atatataccc gcaccatata
    12481 cctttaaaca atttttttaa aagttttttc taaatactta ttttccctgt gtcaataata
    12541 tgtttctata ataatgtccc tttaatttta tcaattcttt atgatttcct gattccacaa
    12601 ttctaccatt ttctaaaact attatattat cacaatttga tatggtactt aatctatgag
    12661 ctataattat acttgtcttt ccataagtta aattatttat tgcttcttga attttagctt
    12721 ctgttgctat atctatgtta cttgtagcct catccaaaat aagtattgga gcatttttta
    12781 atactgctct agcaatactt attaattgtt tttgaccttc acttaaatct tcacccctat
    12841 ccgataatac agtactatat ccttcacgca ttttaactat aaatttatgt gcatttgcta
    12901 ttttactagc atatataact tcttcatcag tagcctccag atttccatac cttatatttt
    12961 ccataactgt atcattgaat atataacctt cttgtggaac tactccaaaa caagttctca
    13021 aatagtttct gttaatagag tatatatcct taccatctaa aaatatactt ccttcatcta
    13081 tatgataaaa atttgtaatt agatttataa tcgttgtttt tccacttcct gtctctccta
    13141 tgatggcatt tgatgtgcca gctttgattt ttaggtttat attatctaaa attttctttt
    13201 ttgaatcata agaaaaactt acatctttaa gctcaatttc tccctttaca aaatgagagt
    13261 ttaaattacc atctttatta tcgttaatct cacttttaaa catgttatgc acttcttgct
    13321 cttcttgttc atctataatt tcaaaaactc tttcacatcc agctatggct gaaagtaatg
    13381 tattaaatag atttgctaat tcatttaaag gtcttgtgaa ttgtcttaca taacttaaaa
    13441 aactagctat aacccctatg gttataatat ctttagatgc aagaacacct cctataatac
    13501 atatagttgc aaatcctata ttactaatca cattcataat aggcattaaa agagaagtgt
    13561 aaatttgcga tttaagggaa atatttagaa gtctatcatt gacttcttta aaatcattta
    13621 cagattttct ttcataatta aaagatttta ctacatttat gtttgatatc atttcttcaa
    13681 tatgtgcatt tagttttccc aactcaactt gttgttgctt aaaaaaaact ctagttctct
    13741 ttgcaataaa attactcaca aacattacta tcggtattaa tattacactg caaaatgtta
    13801 aaattgggct taatacaaac atcataataa aagtcatcaa tatgtttaag attcctgtaa
    13861 ttaactgtac taatgaattt gatattcctg tactgatatt atcaacatca tttgtcactc
    13921 tactcataat atctccagta gaacttttat caaaaaaggt tattggtaga tttttaaatt
    13981 ttaaaaataa attacttctc atttctttaa ctatactttg agataatcta gctgtacata
    14041 tccccttaga cagtttaact gaaaactcaa ttatataaaa tgaaagtaaa ataattattc
    14101 cttttaagaa aatggatttt gaataagtat cgatatctat tatgtcaact acctttccaa
    14161 tagtatatgg tattacagtt gatataattg aatcaattat tactagcaaa aaaataaatc
    14221 tagtaatctt cttatctttt ttaaagtaca taaaaattct tttaaacgtt tttttaaaat
    14281 ctttaggctt ttcttttttc tttaatctac tgaccttttg atttccagta cttaagttca
    14341 atatacttcc tccttaattt acatcttcac ttactaaatt ctgcaacgca aaaatttttt
    14401 tataaattgg agaactaacc agtaaatctt catgtgtagc aaatccaacc acctcaccct
    14461 catccaagac taaaatctta tcacagtcaa ttatagaact aatcttttgt gaaatagtta
    14521 ttacagtaca tttcttactt tccttttctc ttaaattact tatatactct tttatattct
    14581 ttcttataat tgtctctgta ttaatatcca ttgcactaaa acaatcatca agaattaata
    14641 tttgtggttt tttggctata gctctagcta tagtaattct ttgtctctgt cctcctgata
    14701 aatttttacc cctttgaaat aaaacctcat cataagaatt ttcgagttta gttataaatt
    14761 gatttgcatt tgctatctca catgatttta taacatcttc tctacttata ccctcatttc
    14821 ccataactat attttctagt atactttttc caaacacact gctattttga aatactacac
    14881 ctattttttc ccttaaactt tcttcagaaa tactgtatat actacgtcct ccaatcttaa
    14941 tatcaccttt atcaattata tataattttt gaattaaatt tactaaagaa gtctttcctg
    15001 aaccagtttg accaatcaca ccaatatttt gaccttcttc tattttaaag gatacgtttt
    15061 ttaatatttt ttcatctgta tttttgttat acgaaaaatc tacattttca aaaattatat
    15121 cacaatttac aaactttaaa tctttttctc caacatctaa actttcttct ctctcaatta
    15181 cttcttcatt ctcatcaacc aaaacctcac ttattctctc ataagatgct tttgccctaa
    15241 caaaaacatt aaaaacatgt gaaatcatta taagagatgt taatattcta gtcatatagt
    15301 ttacataagc aactataatc ccaattttaa tttctcctga ataaattaaa tgccttccaa
    15361 tcagtaatat tattattatg gaaacattaa tgattattgt aattgtcggt ctaaaaaatg
    15421 acatcatagt tgtagctttt ctactatttt taaaaagttc atcatttaac tcttcaaagc
    15481 tttggttctc ataatcgtat ctacaaaata gtttaactac ttttattcca cttaaaaact
    15541 gtcttaattt tgaatttact ttatctgtac tttcttgtac atttttaaat aatctatacc
    15601 ctatcttcat attaagcaat attataataa atataattaa aactgcaatt agaataatta
    15661 ttgacatctt taaatttaat tttatagcta taattaaact tcctatacac attattggtg
    15721 actttataaa aactctcatc attccattaa cgaattgttg catttgattg atatcattag
    15781 taattcttgt tattaacgaa gctctaccaa atttatctat ttgttttaat gagtatgtat
    15841 ttatcttttc aaatacatcc tgtcttaaat cataagaaaa atgctgagaa actcttgccg
    15901 agattacact tcttattatt gcaaatattg cacctaaaaa agttatacct atcattatag
    15961 ctcctgttga tagtatgtag tctaggttct tatttgaaac ccctatatct attatttttg
    16021 ccattatagt tggttgagct aaatcacaaa aagcttccaa tgttaaaaaa aatactgctg
    16081 ttagaaataa tactatatat ttttttatgt attttttaaa atacttcatt caatcatccc
    16141 ttcatagaat tttctcatta ccaaaagtat atcataatgg ctaatttaat tgattatatc
    16201 aattataaat atttgattta ttattaattt ttataccata tattttataa attatttgtt
    16261 tttatttcct atatttatat atatttatag
//
LOCUS       DAMKPG010000083        15959 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMKPG010000083 DAMKPG010000000
VERSION     DAMKPG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.087"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="PC621_002813"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="PC621_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF4109960.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="PC621_002814"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="PC621_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF4109961.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="PC621_002815"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="PC621_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF4109962.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="PC621_002816"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="PC621_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF4109963.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="PC621_002817"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="PC621_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF4109964.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="PC621_002818"
     CDS             2645..3151
                     /locus_tag="PC621_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF4109965.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="PC621_002819"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="PC621_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF4109966.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="PC621_002820"
     CDS             3966..4754
                     /locus_tag="PC621_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF4109967.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="PC621_002821"
     CDS             5225..8941
                     /locus_tag="PC621_002821"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF4109968.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRKLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLMNSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="PC621_002822"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="PC621_002822"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF4109969.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="PC621_002823"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="PC621_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF4109970.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="PC621_002824"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="PC621_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF4109971.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="PC621_002825"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="PC621_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF4109972.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6246 a         1985 c         3249 g         4479 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaaaat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttatgaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa taatcagcta gatgagatat atctcatcta gcacaagat
//
LOCUS       DAMKPG010000084        15534 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMKPG010000084 DAMKPG010000000
VERSION     DAMKPG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.088"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            860..1621
                     /locus_tag="PC621_002826"
     CDS             860..1621
                     /locus_tag="PC621_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF4109973.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            1686..2468
                     /locus_tag="PC621_002827"
                     /pseudo
     CDS             1686..2468
                     /locus_tag="PC621_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            2735..3154
                     /locus_tag="PC621_002828"
     CDS             2735..3154
                     /locus_tag="PC621_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF4109974.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(3257..3523)
                     /locus_tag="PC621_002829"
     CDS             complement(3257..3523)
                     /locus_tag="PC621_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF4109975.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            3750..4451
                     /locus_tag="PC621_002830"
     CDS             3750..4451
                     /locus_tag="PC621_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109976.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            4533..5141
                     /locus_tag="PC621_002831"
     CDS             4533..5141
                     /locus_tag="PC621_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF4109977.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            5465..5929
                     /locus_tag="PC621_002832"
     CDS             5465..5929
                     /locus_tag="PC621_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HDF4109978.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            6566..7582
                     /gene="gap"
                     /locus_tag="PC621_002833"
     CDS             6566..7582
                     /gene="gap"
                     /locus_tag="PC621_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF4109979.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            7977..9146
                     /locus_tag="PC621_002834"
     CDS             7977..9146
                     /locus_tag="PC621_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4109980.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            9527..13780
                     /locus_tag="PC621_002835"
     CDS             9527..13780
                     /locus_tag="PC621_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF4109981.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            13929..14423
                     /locus_tag="PC621_002836"
     CDS             13929..14423
                     /locus_tag="PC621_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF4109982.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            14539..14916
                     /locus_tag="PC621_002837"
     CDS             14539..14916
                     /locus_tag="PC621_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF4109983.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            15122..>15534
                     /locus_tag="PC621_002838"
     CDS             15122..>15534
                     /locus_tag="PC621_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF4109984.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
BASE COUNT         6335 a         1530 c         2730 g         4939 t
ORIGIN      
        1 agtaatgtta ttaaaattaa gtatatagtt aaaagtgtat agcaaaaagg ctgttatatt
       61 aatgttagag gttcagggaa aatatataaa gcaaagatat ttacagataa tgtagattgc
      121 acagctataa gttagataat agaattatgt aatcaagaat ttacaacaaa gtataagata
      181 aggataatgc taaattgcca tacaggtgct agatgtgtaa atggatttaa ttaatactat
      241 tgataaagtg caacaagcac ataaaccaat tgatgagatt attaaaacta taagagatat
      301 agttgacata attaaaatat tggaacctat ttataatttt aaatcaaatc aataatgatt
      361 aagattaaaa taatattaag gtatcaatgt tataagagta caatataagg agtactatat
      421 agtactcctt acttatacat attaggcaat aggaaatctt ttcatagtta aattttcaaa
      481 tattttttag ggaactttat aattgagaat taagggtata gtatgtgatg aacttactaa
      541 atttataatt aggttatata atggaataat catcaatagt tttactttag ggaaaatata
      601 tgcttaagga agtatagctt actaatatta ttaaaatgta tatttaaaat gataaaaaaa
      661 gaattagaca agtatttagt aaattgatta tgtaaaattc tatcaagcag gaaccctatg
      721 actttagtca tgggaggttc agtaggtaac ttgttgaatt aagtttatat tatttaaata
      781 aactacttaa tttagataat ataaaaagtc agaattttaa gaatagagct atataatctt
      841 agctattagg agaagtacta tgataggaat atattttagt ggaacaggaa attcaagata
      901 ctgtgtagaa aaatttttac aagaatatga tataacagca aattcttatt ctattgaaga
      961 taatgaactt ttacagcaca tcaataatca tgagaatatt atttttagtt atcctgtgca
     1021 atatagtaat gtgccaaaaa tattgaaaga ttttataata aatcattcaa ctttgtggaa
     1081 tggtaaacgt gtttttatta ttgcaactat ggctctattt agtggagatg gctctggtgt
     1141 gttagctaga atattaaaaa aatacggtgc tataactgtt ggtggattac atgtgaagat
     1201 gccagatagt ataggagatg aaaaggtatt aaaacattct ttagagtata ataaaaagct
     1261 tgtaataaaa gcagaaaaga aagtaactaa agctgctaaa aaactgaaac atgggtcacc
     1321 accaaaagaa gggttaggtt ttttgagtca tattgtaggg ttatttggac aacgattgta
     1381 ttttatcaat aaaacaaata tgtatacaga taaactaaaa attaatactg aggaatgtat
     1441 tgtatgtggt aaatgtgtta atttatgtcc aatgaagaat ctagtaatta aaaattgtat
     1501 gattgttgca aatgggcaat gtacaatgtg ttatcgttgt gtaaataatt gtgcaaaaaa
     1561 agcaattaca ttgttaggta aaaaagttgt tgagcagagc aatattaaaa aatatatgta
     1621 agaaatttaa agttttattg aatgtaaatt gatgtatatt ttatgttgga aagagaggtc
     1681 aaataatgga atactacata gttgattcat ttgcaactaa actatttaaa ggaaatcctg
     1741 ctggagtttg tgttcttgac agaataatac ctttagagtt aatgcagaaa atagcagaag
     1801 aaaacaattt accagaaaca gcatttgtag ttaaaggtaa aggaaattat gaattacgat
     1861 ggtttactcc taaagcagaa attgatttat gtggtcatgc aactttagca gcagcttatg
     1921 taatatcaaa ttttattgat gttaatgtaa aaaggataga tttctttact caaagtggaa
     1981 agcttgaagt tacacgaaat ggaaatctat atgaaatgat atttccagaa ataatgccta
     2041 ttgaaataga gttatcacca taacaagcta atttgatagg ctgtgtacca agtgctgtct
     2101 attcttcaag agatttaatc ttattgctaa acagtgaaca agaagttata aattataaac
     2161 ctaattacgc acaattgcgt aaattgacag actggttagg tataataatc acaccacaag
     2221 gaagtaatac agattttgta tcaagatatt tttgtccaga attagattca gaagaccctg
     2281 ttactggttc atcacattgt aattttattc catactggtc tgaaaagttg ggaaaacaca
     2341 aaatggtagc tgctcaactt tctaatcgtg gtggtataat tcagtgtgaa gtgttgaagg
     2401 ataatactgt aaaaatttct ggtgaagcag tcttgtttat gcagggaaca ataaagattg
     2461 atatttaaat aagtataata actaatttat tatgaaaaat aattaatttt cttagtgtta
     2521 acaataataa agtagagtta aaaagcttga tttcacttaa atcaatatga tagatgttga
     2581 tactaagatt gacaagcatg attaaaaaag ataatattta atctataaag ataaaaacac
     2641 agtctggttg gtagtccaga cgcagcaata gctgtcagta acctgcctcc ttggttgtcc
     2701 gttctttaga aagtaattta aggaggaact agcgatggac aaaattagtg gaaaattaac
     2761 agtcttattt gaagaaccat tttgggtagg tatatttgaa agacaagatg gtaaaaaata
     2821 tgaagcatgt agggtagttt ttggagcaga acctaaagaa gtagaagtgt atgaatttat
     2881 acttgaaaga tttttctcat tagattttgg tagcattaaa ttagaaaaaa atgtgaccaa
     2941 agacaacatt ggatacaaaa gaatgcagag aaaagtaaaa aaggaacaag aaaaagaaac
     3001 tatagggact aaggctcaaa atgctcttaa gcttcaatat gaagaaagaa aacaggatag
     3061 aaaaaaccta gctaaaaata gaaaagaaga agaaaaagaa agaatattta atttgaaaca
     3121 agaaaagaga aaagctaaac ataagggtca ttaattttaa ttaaatatct ttttaataat
     3181 tgctagaaaa aggtgtataa agtttttaat tttatacacc ttaatttatt gaaaagaata
     3241 aggttttata tttcaattac tttaaaatat aaaaatatac taataaaaat tgtaaaaata
     3301 gtataaatgg tataccaaat gtaaatttaa tgtgtttagt tttatgtcta aaagaataca
     3361 ttccaatcat agaacctata ctacctccaa ttactgctat agacataaga gtagcttctt
     3421 ttattctcca ttcgtttcta attgctcttt tcttatctat gtacattgaa aagaaagcaa
     3481 tagaattaat cactataaaa tatattagaa taaaattttt cacagtcttg cctccataaa
     3541 aatatattat acattataaa acaagatatt tgttttggaa agttaaattt gtaattagta
     3601 catataaaat atctgataag aataaatata atattaattg gaaaatttaa aaaaattaaa
     3661 caattagcaa atagggggta tatttataag acacaaagtg ataaaatcta tttagaaaat
     3721 aatgtaaaat aacaggagga taaagcaaaa tgaaatttat agtagaaaaa gaagtttttg
     3781 ataaattaga aaatgtatgc tttggtgtag tagttgcaaa aggaatagat aatactaaag
     3841 aaatagaaag aataagcaac cttttagata taagtataga tagagtagaa gattacttca
     3901 aagataaaaa agtaaaggaa tcagaagaaa ttataccata tagagaggca tttagaagtc
     3961 taggcatgaa cccaaataaa tttatgagtt cgatagaagc aatgacaaca agggttgcta
     4021 agaataagaa acttcctcat ataaatccta ttgtagattt aggaaactct atttctttaa
     4081 aatatttact tccaatgggt gctcatgata tggattttag aaatgatgat gtatatgtta
     4141 gattttctaa aaaaggggat aaatttgtac catttggaga aactgatgtt gaattaatgg
     4201 aagaaggaga attaatttat tcagttggag acatggtaaa aactagaaga tggatatgga
     4261 gacaaggaga ggaaggaaaa attactaata gctcaaaaaa tatatttttt cctatagatg
     4321 gttttactga tgctaattta gataaggtaa tgagtgcaag agaagaatta gcaaaattgt
     4381 taaaggaaat atttaattgt gaaatcaaag taggttttgt agataaagat aatcctgaaa
     4441 tggaaatata ataagttgtt gaatacttgt taagatatta tatttactta atgatgttaa
     4501 aaaggtatta aaaaagaaag gtcgtagtaa atttgaaaaa aactataata aagaatgtaa
     4561 atccaatgat tgaaaataag tttataggac tttttgaaat agagtataaa aacaaattag
     4621 atgaagataa ggtttggatg gtagcatcaa gaaaaagtag tgagcaatta aaaaatattt
     4681 atttgaagaa tgaagaagat agtgtagatg cagttgtaat agttgggctt cataaaagta
     4741 gtaaaaaatt aattttaata agacaattta gagtacctat taatggatat atatacgagt
     4801 taccagcagg tcttgttgat gaaggagagt ctatagatat ttctgttgaa agagaactta
     4861 gagaagaaac aggacttact cttttagaaa ttaaaaaaaa taaaagtatt gataaagttt
     4921 atttatctcc tggaatgagt gatgaatctg ttgcctttgt ttattgtata tgtgatggtg
     4981 atataacaga tgaattttta gaaccagatg aagagattga agcgctttta atttcgcaag
     5041 aagatgcaaa agagatttta caaagtaatc ataagataga cacaaaagca tttttgattt
     5101 tacaaatgtt tgtgagttta ggagctaaat tatttgaata gatatataga aaagaggtta
     5161 tatagaagag taaaataata aagtttattt tacttgtata aattataatc tcttttttta
     5221 tacaataaag tataattttg tgtattttta aataagttac ttgactctta aattttcata
     5281 atatgttata aaccaattta agatactaca ataattaaaa ttccaaaatt atttagcata
     5341 tttgcaatta aaatgagaaa aggtaaaggc tgaatgattg aaaaatttga tgtaaaatag
     5401 tagattagtt taatattata gtttgtaggg ttaaaaatta gaaattgtaa aggagataaa
     5461 ttctatgaaa aaattagtta ttattttaat tagtatcatt agtgtggtta gcttgacagc
     5521 ttgtaaaaaa agcaatgaaa gtaaatataa taatttaaac aacaaagaat caggacaaac
     5581 aaccattgaa atggacttag ataaaaatta tgatacttca gacccatttg ttaatgcacg
     5641 tttgttttgt gtgtctaatg atatagatgt tttagacaca gagatttctt ttaaaatgga
     5701 tggagatagt ggtattgtgg aaataaagga caataaaaca gatgaaactt tatggagtaa
     5761 tacatggcat ggaagcgttg ataatgatac attcgcaatt tctctttcta atatacaaaa
     5821 agagaaagag tatgctatat gctttaccgg aattaaaata aatcatgcag ttgtaaaggt
     5881 tacttttgaa agcaatttag ttaaggagaa agaaaaaccc tcaaaataaa ttccagcttg
     5941 tctataactt gatattcagt acataaaagc agttgatatt tatgattgac tgctttttcc
     6001 tatcaaaaag gataggttgt gcatttttac ttgaaatagt ttatgcacat caaaagttgg
     6061 tgagcatgat attggaaatg gtaagcatga aatcttgata acacaaggaa ataaatttaa
     6121 gttttcaatt aagtatattt ccataattta gttaacaata agctaattat tatttatatt
     6181 ttgatatata aataataatt gtggcaaatt ttaaaaaatc atatattttt tgaactaaat
     6241 aaaaatttgt ttttattatt ttaaattaga ggagattctt gaagatatat tatttttaaa
     6301 aataagtata gtttaaaaat tcaaaaaaaa gatggcaaaa tatgtcccaa gggacattga
     6361 gtaaaataaa tgatttcagc ttatttacac taaaaagtac atactaaatg aggggtgaaa
     6421 taaagaaaaa tgatatacta attgtctttt gataataaaa aaacatggaa tttgattatt
     6481 taatcaagaa aaagtattga acaaatgatt tatttagtgt acaataatca tgtaaaaaca
     6541 tagtaatgga gggagacatt tcataatgaa aattaaggta ggaatgaacg gatttggaag
     6601 aataggaaga gctgtattaa gaatagctca agaagaatta ggagataata tagaaattgt
     6661 agccataaat gcaagagcaa caacagaatc tttggcacat ttatttacat atgattcatg
     6721 ctatggaact tttagaggtg aagtagaggc aaaggatgaa gacactctag tagtaaactc
     6781 taaagagata aaaatattaa gatatagtga cccagaagaa ttaccatgga aagaactggg
     6841 ggttgatata gtaatagaat caacaggatt atttacacaa agagaaaagg cagaaaaaca
     6901 tattaaagca ggagcaaaaa aagtaattat aacagctcca ggaaaaaatg aagatataac
     6961 tatagttatc ggagttaatg agaaagaata tgataatcaa aagcacaata taatttcaaa
     7021 tgcatcttgt actacaaatt gtctagctcc ttttgctaaa gtattggacg aaaaatttgg
     7081 tatagtaaaa gggttaatga ctacagtaca ttcatatact aacgatcaaa gaatattaga
     7141 taaaagccat aaagatttga gaagagcaag agcagcggct gaatctataa tacctaccac
     7201 tacaggagca gcaaaagctg tgtcaagagt actaccacaa ttagaaggaa aattaaatgg
     7261 tttttcactt agagtaccaa caccaacagt ttcacttgtt gatttagtgt gcgaacttaa
     7321 agaaaacgtt acatctgagc aagttaactc tgtgttaaaa gatgctgctg aaggagaatt
     7381 aaaaggtgtt ttaggatatt gtgacaagcc acttgtttct atagattata aaggagattc
     7441 aagatcatct attatagacg ctctatccac aatggttata gaagacaata tggttaaggt
     7501 agtttcttgg tatgataatg aatggggata ttcttcaaga actgttgact tagtaaaata
     7561 tgtagctgaa aaattaaaat aatatatatt tcttaaagca aattttaaaa aaattttttg
     7621 aaaaaattat ataaaactaa aaaaaaagat tatgaaaaat attgaaggaa aaattttttc
     7681 ataatctttt ttctttcatg caaaaagata caagttacaa cttatatttg catattaggt
     7741 cgttagagaa gtatatatat atttagtaag gaatcatact ttttataaaa tgaaagataa
     7801 gatgtcgaaa acttgcataa atctgaaaaa tttttattat taaaatactg attttcattt
     7861 taaaaatgat aaaaaaatga caaataaagt tgcaaatagt aatacttata agtataatta
     7921 aaaaagagaa aaggttttgt taaactttat aacaaaaaaa gaagggatga ttcaaaatgg
     7981 agaagttaac agacaggaga caggttaagg cagtaagcat ggaaaccttc attttcctaa
     8041 tattactagt agttggtttt gggtatgttg gtagtattat gggagcagga atgatgttca
     8101 aagtaataat gagtacagca catgctttac tattggagac agttttctta attatggcta
     8161 tggcagttct tgcaggcgca ttaagcgcac tactttcaga gtttggagta atagcgttaa
     8221 ttaataaaat tttcgctgta tttatgaagc ctttatatgg actacctggt gctagtatag
     8281 caggagctat aacaacttat ttatcagata atccagctat tatacctttt gctaaagata
     8341 aaacattcac acagtatttt aaacaatacc aagttccagc attatgtaac ttaggaacag
     8401 cctttggtat gggacttatc ttaacaacat ttatgatttc tcaaggtaca gaatatgtac
     8461 ttccagcatt aattggtaat ttaggtgcaa ttataggtag cgtcataagt gttagaataa
     8521 tgttgacatt tactaagaag ttttataaat acaatccgga agaagataaa gcaacaggaa
     8581 cattagagaa aaaggatgaa tttagagaaa tcagagaagg aaatgtattt caaagagctt
     8641 tagatgcaat tcttgaaggt ggtaaaatgg gggtagatat gggaatggct ataattcctg
     8701 gagtcctagt agtatgtaca ttggtgatgt tattaacttt tggaccatct acagaccctg
     8761 ttacaggtca agaagtttat acaggagctg catatgaagg aataaaatta cttccagtta
     8821 ttggagataa acttggattt atattagaac cattatttgg atttacatca ccagaggcaa
     8881 tagcattccc tataacttca ttgggagctg ttggtgcagc aatgtctcta gttccagagt
     8941 ttataaagtc aggagctata actcctaatg atatagcagt atttacagct atgggtatgt
     9001 gttggagtgg atatttaagt acacatattg gtatgatgga tgcattaaat gctagacagt
     9061 tggcaggtaa agctattctt tctcatacaa taggtggttt atgtgcagga gcagcagctc
     9121 actttatatt tacactcgta ggataaacgt aatcagctaa gataaaaaat aatatacaaa
     9181 aaaatggttg ttgatatgta ataagaattt acatatcaac aaccattttt ttctgactaa
     9241 agataattta taatacataa acattaagtg gatattagac ttaagttata tttatgtaaa
     9301 aagtaacaaa aatctgtaga aatttatgac aaaatttagt acactaacta tatgttctta
     9361 tttaaaattt aaggttagaa ttaggaatta cctagtagag taagctttga ttaataaaag
     9421 aatcataaaa taaaaatatt agtcataaag attataaata acttacttaa aataaagcca
     9481 tagaacttac aaaaattaaa aaagcatata aatatggagg ggtacaatgg atgaattatt
     9541 tcatatagga attgatgtag gttctacaac ggtgaaggtt gtggtactta ataatagtaa
     9601 taatatagtt cataaagaat atagaagaca ttattctgat gtaaaaaaat cagttaaaga
     9661 agtactaaat ggtatatatg aaaaattggg agatataaat actaccatta taataactgg
     9721 ttcaggtgga attggaatat caaaaaaatt aggtgtaaaa tttgttcagg aagttatatc
     9781 tagtacaaaa tcaatagagt attttcatcc tgaaactgat gttgtcatag aacttggggg
     9841 tgaagatgca aagataacat atctaagtgg aggaatagac caaagaatga atggaatatg
     9901 tgcaggtggc actggagcat tcatagacca aatggcatca cttttaaaga cagatgccag
     9961 tggattaaat gaacttgcta aagggtataa tgttatatat cctatagctt caagatgtgg
    10021 tgtttttgct aaaacagata ttcaacctct tataaatgat ggagccaatc agactgatat
    10081 agcaatgagt attttcaatg cagttgtcgt acaaactgtg agtgtccttt cttgtggaag
    10141 aaaaatagaa ggaaatgttg cgtttttggg tggtccattg tactttttat ctgaacttag
    10201 agaagcattt aaaagagtat tggatttaaa agatgaaaac ataatcttcc cagaaaatgc
    10261 acaactttat attgctattg gagcaggatt attatcagta gatgaagata atatacaact
    10321 aaaatctcta atagaaaaat tggatagtat aaagaatatt gaagatggag aagtaaattt
    10381 attagaacct ttatttaaag ataaaaatga gtatgaagaa ttttcaagaa gacatgaaaa
    10441 agaaaaaata agttatgtgg acataaattc aataaaaagc aattgttatt taggaattga
    10501 tgcaggctca actacaacta aagcagcact tattgatgag gatggaagac ttgtatattc
    10561 ttattataat agcaatgaag gaaatccatt aaaaacaacc attaaagtta taaatgaagt
    10621 atatgatata ttaccaaaaa atattaaaat attgagttca actgtaactg gatatggaga
    10681 aggtcttatt aaaaaagctc ttaaaatcga taacggagag atagagacta tagcacatta
    10741 caaagctgct aaatttttca ataaagatgt agattttata cttgatattg gtggtcaaga
    10801 tatgaagtgc ctaaaaataa aagatggagt aattgataat ataatattaa atgaggcttg
    10861 ttcatcaggt tgtggctcat ttttagagac atttgcaagt tctttatcta tgacaataga
    10921 agaatttgca catgaaggta tttattctca aaatcctgta gatttaggtt caagatgtac
    10981 tgtatttatg aattctagag taaaacaagc tcaaaaagaa ggtgcaagtg ttggagatat
    11041 atcagcaggg ctatcttatt cagtaataaa aaatgcttta tttaaagtaa taaagattag
    11101 agatttaaat gagattggta ataatattgt tgtacaaggt ggaacctttt ataatgattt
    11161 ggtgcttaga agctttgaaa aactaattgg aaaaaatgta attagaccta atatttcagg
    11221 aattatggga gcttttggtt cagcacttat agctaaagaa aaatatgaaa gtggatacaa
    11281 aacatccttg ttatctaagg aagagttgaa taatatcaag ctagaagcaa gtgtaactag
    11341 atgtaaaggt tgttcaaatc attgtcttct tacaataaat aaattttctg ataatgaaat
    11401 ttttatttca ggtaatagat gtgagaaggg tgaagctatt tatggtgata aaaaaatagg
    11461 attagaaaag aaaaaaccaa taaatttatt taagtacaaa tacaatagaa tttttagata
    11521 caaaccattg gaaaaagaag aagcaaaaaa tggggaaata gggataccaa gagtattaaa
    11581 tatgtatgaa gattatccat tttggtttac ttttttcaat gctatgggct ttagagttgt
    11641 actttcagat aggtcatcaa aacagttata tgaaactggt ataactagta ttgcatctga
    11701 aacagtttgt tatccaggaa aattagttca tggacatata gaaaatttaa ttgcaaaagg
    11761 tataaaaaat atattttatc caagtgtaac aaatgaaaat aaagaggatt caaatgcaga
    11821 taattattat aattgtcctg ttgtaatatc atattctgaa gtaataaaaa acaatgtaga
    11881 gaatataaga aataagaaca taaattatat aaatccattt ataagtctaa atgataagga
    11941 aaaattgaag aaaagactat atgatgaatt atcaagacat ttttctgata taaaaataac
    12001 taaagaagaa gttaatcatg cagttgataa agctactgat gaacagaatt tatttaaaat
    12061 ggagatacaa gcagcaggtg aaagagcact taaagaaata aaagaaagaa atatgaaggg
    12121 gattgtgtta tgtggaagac catatcatat agacccagaa ataaatcatg gaatgccaga
    12181 gcttataaat tcattagata tggcagtgct tactgaagac tctatatgtc atttagccaa
    12241 tgtacaaaga ccacttagag ttgtagacca atgggtatat cattcaagat tgtataaagc
    12301 agctagtttt gtaagagata aaaactattt ggaacttgtt caacttaatt catttggctg
    12361 tggattagat gcagtaacta cagaccaggt tcaagaaata cttaatgaaa aatcgaagat
    12421 atatactatt ataaagattg acgaaggcaa caatttagga gcagctaaga taaggatgag
    12481 gtccttaaaa gcagctatgt ttgagagaga aagcaaaaat atagatgtaa aaaatataaa
    12541 ggtaaacagg atacagtatg ctaaaaataa tcagataacg aacaaacata ctatattagc
    12601 accacaaatg tctccaatac atttccaatt ccttgaaaaa gctgttaact taagtggtta
    12661 taatatagaa attctaaaag atactgatag tagtgtaatt gaagaagggt taagatatgt
    12721 aaataatgat gcatgttatc cagctataat agttgtaggt cagctaataa cagcattaaa
    12781 atctggcaaa tatgatttaa ataatacttc tgttactata actcagactg gtggaggttg
    12841 tagagctaca aattatattg gattcttaag aaaggctata tatgatgctg gatttaaaga
    12901 tgttcctgtc attgcactta gtgtaaatgg aatagaagat agtggtataa tggataatat
    12961 atcactaaaa ttaatcaata gactctttat gtctgttgtt tatggtgatt tgctgatgaa
    13021 agtactatat agagtaagac catatgaaaa agttactgga agtgcaaatg ctctatatga
    13081 aaaatgggtt gatatatgca aatcttctct tgttaaagca aaaatttctg tatttactag
    13141 aaatataaaa gatatagtaa aagaatttga taatttagag atactagata tcaaaaaacc
    13201 aaaagtaggt ttggttggag aaatattggt gaaattccat ccaattgcaa ataataattt
    13261 agtagatata ttagagcgag aaggtgctga agcagtcgtg ccagacctta caaatttctt
    13321 cttaagttgt gcctttaata ctatatacaa acacactcat ttagaaggaa gtagaaagag
    13381 tagaatgata ggagaagcat ttatatacat aactggaata tatcaaagag tatataaaaa
    13441 agcacttgat aagagtgaga gattttatgc tccagccaat ataaaaaatg ttgctaaaag
    13501 tacagaacca gtagtgtcat taggtaatca aactggagaa ggttggcttt taacaggtga
    13561 gatggttgaa ttattaaatg aaggtgtaga aaacattatt tgtatgcaac cttttggatg
    13621 tttacctaat cacataattg gtaagggttc tataaaagaa ttaaagaggt tgtataaaaa
    13681 tgcaaatata atacctattg attatgaccc ttctgcaagt gaggtaaatc aacttaacag
    13741 gataaaactt atgctttcta aggcttttaa aaatatataa gatttatatt ttgatatgtt
    13801 aaaagttaat gaatttaaaa atatactcta tattaagtat ctataaaaaa gagttatcct
    13861 attttttagg gtaactcttt attagttaaa aaattatatg tatactgata ttaaaggggg
    13921 gaagtaatat gagtttagta tatgatgttt ataaatcaaa attaggaaat ttgtacatat
    13981 taagtgaaga tggttttata gttagtgtat acataggaga tgaaaaattt aataatttaa
    14041 aaaatgaatg tattaaaaaa ggaattgata taaagataag taaggaagat ttaaaggaag
    14101 caataagcca gatagatgat tactttaatg gaaaaagaaa agtctttaat ttaaatattc
    14161 gtattgaagg gactgaattt caaaaatcag tttataggga aatgttaaaa ataccttatg
    14221 gagaaacatt gtgttattca gatatagcaa gaaatataaa caatccaaaa tcagtaagag
    14281 caataggaca agcaagtaaa agtaataaaa ttccaataat aataccttgt catagagtag
    14341 ttggcaaaaa aaatattggt ggatatatgg ggaatcattc tgatttgaaa gagatactat
    14401 taaatttaga aagacagagt taacttagaa agaaatgttt ttattgttaa tatttgatat
    14461 aataatagta aaaagaagta ttttttacaa aaacaatgaa gaatttattt taatttacaa
    14521 aaatgagggg gaattttaat gtgtgataaa gttatagcat caaaatatca tgagaaggga
    14581 tttaattgtg cagaatcagt tataaaagca tataatgagg aatttaatac agatatacca
    14641 gtttgtttag gaagtggact gggttcaggg tgtggggttg ctagtttatg tggtgcagta
    14701 aatgcatcaa atataataat tggatacgta aaaggcagaa accacgaaga tgaaagtact
    14761 aaagctaaag tatatgctaa agatttaact acaacagtaa gaaaagagta tggttcagag
    14821 ttatgtatag acctaaaaaa agatttagtt gcttgtagag aaataatgga tttcgcatat
    14881 gatagtctaa aggaaacttt aaaaaaagaa ttataagaaa ataagctatc aagtataaat
    14941 tatttgatag cttattttta tagtcgatac taaataaatc aaatatattt tagaattatt
    15001 atttgtaaat aaacagcaaa taattttagg atttttattt aatatagttt ttatttatat
    15061 ttatttttga atatgttata ctttgtttaa ataaatattt gtaatgaggg agatgagaag
    15121 attgaagaat ctcaatttac ttattaataa attatattca aaaaatcata atgaagcata
    15181 taaaactttt ttattcttag aaaatgaaag tttaaaatca aatattacat attgtttttt
    15241 tgactcattc ctagaaatga ttgataacga aaactcatat attcgaacaa ggggattgtt
    15301 attaatctct gcaaatgctc agtgggatat tgataataaa attgaaataa atatagatag
    15361 tatactaagt catatcgtag ataaaaaacc ttctgtatca agaatgttta ttaaatccat
    15421 acctaatatt acaaagtata aagaaaatct tattaatcgc ataaaaatgg aattaagtaa
    15481 tgcagatata agtatttata ataataatat gaaaccatta gtagaaaagg atat
//
LOCUS       DAMKPG010000085        15492 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMKPG010000085 DAMKPG010000000
VERSION     DAMKPG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15492
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.089"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            42..377
                     /locus_tag="PC621_002839"
     CDS             42..377
                     /locus_tag="PC621_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4109985.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            514..1791
                     /locus_tag="PC621_002840"
     CDS             514..1791
                     /locus_tag="PC621_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF4109986.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1912..3300
                     /locus_tag="PC621_002841"
     CDS             1912..3300
                     /locus_tag="PC621_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF4109987.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3671..4384
                     /locus_tag="PC621_002842"
     CDS             3671..4384
                     /locus_tag="PC621_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF4109988.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4502..5371
                     /locus_tag="PC621_002843"
     CDS             4502..5371
                     /locus_tag="PC621_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF4109989.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5526..6671)
                     /locus_tag="PC621_002844"
     CDS             complement(5526..6671)
                     /locus_tag="PC621_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF4109990.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(7004..7780)
                     /locus_tag="PC621_002845"
     CDS             complement(7004..7780)
                     /locus_tag="PC621_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF4109991.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8362..9336
                     /locus_tag="PC621_002846"
     CDS             8362..9336
                     /locus_tag="PC621_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF4109992.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9434..9739
                     /locus_tag="PC621_002847"
     CDS             9434..9739
                     /locus_tag="PC621_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF4109993.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9895..11055
                     /locus_tag="PC621_002848"
     CDS             9895..11055
                     /locus_tag="PC621_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF4109994.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11105..12007
                     /locus_tag="PC621_002849"
     CDS             11105..12007
                     /locus_tag="PC621_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF4109995.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12087..12980
                     /locus_tag="PC621_002850"
     CDS             12087..12980
                     /locus_tag="PC621_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF4109996.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      13033..13214
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13442..14572
                     /locus_tag="PC621_002851"
     CDS             13442..14572
                     /locus_tag="PC621_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4109997.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14644..15489
                     /locus_tag="PC621_002852"
     CDS             14644..15489
                     /locus_tag="PC621_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF4109998.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIKK"
BASE COUNT         5958 a         1719 c         2736 g         5079 t
ORIGIN      
        1 agtttgttta ataaattaaa taaagaaaag aggagaataa aatgtacaac atagatgata
       61 tgatatataa ttgtccagta gaagctttat caaatatttt aggtaaaaaa tgggtggcta
      121 taattatatg gaaaatacaa gaagataaga aacgatttgg agagctacaa agagctatac
      181 cagactgtag taagaaaatg ttagttcaac aactagattt tttaattgaa caggggatta
      241 tagtcaacca aaaaaagttg gttaataatg ttgtagagtc aacatatttt ttaagtgaat
      301 caggattacg attgcttcct gtcatggaaa aaatgattat gtggagcaat aaaaatttaa
      361 attgtgataa atactaaaat ttactctgta agtaatgaaa atttaattaa gctatgagta
      421 gaaaaatgta ttaaaattac ataatttttt agttagattt taatttagaa atgggattgt
      481 aatattggaa ataaaataag gaggaatttt aatttgcaaa atgtaaattt atttttagta
      541 tttatagaag gtatagtatc attcttttca ccatgtatac tgccaatttt gcctatttat
      601 ttgagtatat tgtcaaatag tagtgtagaa aatctaaaag aaggaaagac gagttttata
      661 ggaagttctt tatttaaaaa tactatattt tttgctcttg gaatctcaac aacattcttt
      721 atattgggtt catcagtaaa agtattgagt atgtttttta atgaaaataa agatttaata
      781 atgtttattg gtgggattat aattattatc atgggattat tttacatggg aataattaaa
      841 tcttcaatat tgaatagaga aaaaagattt aatgttaagt ttaaagaaat gaaagcaata
      901 acagctttta tattaggttt tacgtttagt tttggatgga caccttgtat tggtccaata
      961 ttggcatctg tattagtcat ggtttcaagt tcaagtaatc atcttagtgc caatctttta
     1021 atagcagtat atactattgg ttttattttg ccatttataa ttacagctat gttttacagt
     1081 aaactattta aaactataga taagataaaa tctaacatgg aaattattaa aaaaataggt
     1141 gggattatac ttatagtatc aggaatatta atgatggtaa atggatttgg aagtataagc
     1201 aaacacttta atacgtcaca aaatagcaaa attgaaagca agcaagaaga aaataagcga
     1261 gaaaattcta ctgataaaga agaaaattct gatggtaata atagtcaaaa agactctaat
     1321 aatgacaata atgataacgg gtctaatgat gaagatagaa taaagtctat tgattttact
     1381 ttaacagacc agtatggtaa aacacataaa ttaagtgatt atgaaggcaa agttgtattc
     1441 ttaaatttct gggctacatg gtgtcctcca tgtaaagaag aaatgcctta tatagaacaa
     1501 ctgtataaag actataataa aaacaatgat gatgtagtaa ttttaggagt agcttcacct
     1561 aatttaggta gagaaggttc aagggaacat gtagttaact tcttaaaaga ccaaggttat
     1621 acattccctg ttgtacttga tgaagatgga gcattagcat atcaatatgg aattaatgct
     1681 tttccaacta catttataat tgacaaggaa ggatatgtaa ctcagtatat tccaggagct
     1741 atggataagg caactatggc atcatttatt gaaaatcaga gaaataaata aaagtattta
     1801 agtatatgtt tatgaatcaa tttaaggtta caaaaaagtg cctaattgta ccacctaaat
     1861 aatcttgtta tagtaaaagt ataaacaata caagaaaggg ttgaatttta tatgaaaaaa
     1921 acatttgatg ctataataat aggatttgga aaaggtggaa aaacattagc aggagatttg
     1981 gcaaacaggg gattaaaagt agctttaata gaaaagtcaa acaagatgta tggtgggaca
     2041 tgtgtaaatg tggcatgtat accaacaaaa agcttagaaa attcagcaaa cagcgtaaaa
     2101 acaaagaata taaattcttg ggatgaagta caagctgaat atgagaaagc tatagataaa
     2161 aaagaaactt taattacaaa actcagagaa gctaactata ataaattaaa ttcaaatgag
     2221 aatgtaacta tattcacagg aatgggaact ttcattgatg aaaaaacagt tcaagttaaa
     2281 actgaaaatg aaatttatga gttagtagca gataatatat ttattaatac tggttcaaga
     2341 ccatttatac ctaatattaa gggaattgaa aataagaata tagtatatga tagtgagtct
     2401 cttatgaatc taagagtttt acctaaaaaa atgacaatta taggagctgg atttataggt
     2461 ttagagtttg ctggtattta tagttcattt ggagcagaag ttacaatatt aaactctaat
     2521 aatggcattt taccaaatga agatgttgag gattcagaag aaataataaa attacttgca
     2581 aagagaaatg taaaaattgt taataatgca aatataaaag agattaaaga agtttctgaa
     2641 tcagcaatag tagaatatga agtagatggt aaatctaaag aattaacaag taatatgata
     2701 cttgtagcta caggaagaaa agcaaacact gaagggctag ggctagaaaa tgcaggtata
     2761 gaattaaatg aaagaggatt tattaaagtc tcagaaactc ttaagactaa taaagagcat
     2821 atatgggcta taggagatat aaatggaggt ccacaattta cttatatatc attggatgat
     2881 tatcgcatag ttattaatca gttatttgga gataagacta gaacaactaa tgatagaaag
     2941 aatatcccaa attctatatt tataagtcca gcattttcaa gagttggtct taatgttaaa
     3001 caagctaaag aaaagggata tgaagtatta gtggcaaaga tgccagtaga agcaattcca
     3061 agagctaaac agattggcaa ggcagatggg tttataaaaa tagtgataga taaaaaatcc
     3121 aataagatac ttggagctag tatgatatgt gaaaattcaa gtgagattat ccatcttatt
     3181 caattagctg tagatttaga agtagagtat acatatttaa gagaccgtgt ttatgcacat
     3241 ccaactatga cagaagctct taatgatatt ctatctccta atatgataaa agaagtataa
     3301 cataagttta atatataaac ctaaaattat cattataagt agattttaga aagaggctat
     3361 ctcaaaaaat agagataagt ctctttttta ctttttaaat gataaaaata tatttgattt
     3421 tataaaagaa gattatctta tgaactaaaa agtttatttt aaaacactac ctatttatgt
     3481 tataaactta taaatataat atagaatcta ttattctgta ttattaagtg aggttaagcg
     3541 ttataattaa ttataaatat tgaataatct aaatggataa gataatggta ttaaaaatta
     3601 tcttaaaagg tgaaataatc tgtatttaat gtagtacagt tatatcttta aagcaaggtg
     3661 gtgaaatgat ataaataaga tatctaaaag agggtttgat gatagtgata aaagatggtt
     3721 ttcaagtaaa gaattaacaa gacttataaa ggctaaagaa gaaatagaat ggttattaaa
     3781 tagggattac aaaatagacc cagtcgttac ttttgttggt gatagatatc agttttctat
     3841 aagacaaaga gatgcattaa aaagagcagt ctgtactgat gaaaaaaata ttataagaca
     3901 atcaaaaagg ctttttctag ataaaatcaa agaagatacc gtaaatatag atggctttaa
     3961 tttgataata tccatagagg tagcattatc tggtggtact ctagtaatag gaagtgatgg
     4021 aaatataaga gatttagcag gtcttagggg aacctataag attatagata aaacagaaga
     4081 agctttgaat ctgataggta aattttttaa taaatacaat gcacagaatc ttaaatttta
     4141 tttagatgct ccagtttcta attcaggaaa tttaaagtat aggatattag agcatgccaa
     4201 aacttgggga attgaaactg aagtagaatt agtaaaaaat gcagatgttg tattggaaaa
     4261 attagataga gtagttagta gtgatgcagt catagttgac aagtgtataa gttattttaa
     4321 tgtggctaga ggtataattg aagagtatat taaagaatgt aatatcataa atttaaataa
     4381 atagataaat agaggtgaga atatataagt atatgtagca atattattag taatacaagt
     4441 aaagtttgaa aataggagaa cgaatacata tcgaatttca taaaatgttg taaaaagaca
     4501 tatgagagga tggatatgtg tgaaattaac taaactaaat attaaaaaat atagagcacc
     4561 tatatataaa tatgctttag caaatgtaaa tttgaggtct gctaaatcta ctaattcaag
     4621 tatcataaca gtaatacctc agggagctaa aatggaagta ttggatgaag aagatgactg
     4681 gattaaggtg atgtacaatt ctcaagaagg atatgtgtat aaggatttgg tatcagtaag
     4741 cgaatatgct tggagtaact taaacttaag agaagataaa tctataactt ccaatataat
     4801 tacagtaatt cctgaaaaat ctagggttga agtccttcaa gtagatggag attggagtaa
     4861 gattgtatat gatgataaaa taggatatgt ttttaattat tttttatcaa ctgatgggaa
     4921 taaaccaaat gaattagatt ataagtattt ttatactgac atgattaaat ttgttaacga
     4981 aaataatatt aaaagtacta gcgattatct aattgtaaca gaccttagaa ataaatatac
     5041 ttatattttt aagaaagata atggaggctg ggggcaactt tacaagtggc aatgtactat
     5101 aggcaagcct gagactccaa ctataacagg tatattttat ataagtggaa gaaaaccttc
     5161 ttttggaacg gatgagtatt cagttaagta tgctactaga attaaaggtg gatattacta
     5221 tcattctgtg ctatatgatt caacaggaag ttatataata gatggcagac ttggtgaagc
     5281 tcttagtcat ggatgtataa gattaagtac ggaaaatgca aagtggatat atgataatat
     5341 accagataca acaacagtta taattcatta agaaaaattt tgagttttta ttgggataat
     5401 gattctcaaa ttaataggat tatatattaa cttcttcata aagagtacta tttaaaaagt
     5461 gtaataagag agtgctatta acaaaacaaa gatattttga ggcactctct tataaatata
     5521 cataattaag atattttcat aaacaattct ataatcttct ccttatcaaa gaaaacagga
     5581 ttagcaccag cacaagcatc tttcatagag ttttctgcaa gcaattcaaa attaggatta
     5641 tcaacaccta catcttttaa agattttggg attccaacct cttcagaaag agcttttatc
     5701 ttatctatta caaagtcaac acactcttta tctgatttat tatttacatc cattccaata
     5761 acttcagcta ttggtcggaa tttggcaggg gctgatttag cattttcctc ctcaactatt
     5821 ggaagtagca tggcattaca tactccatga ggtaaatcat ataaacctcc taattgatga
     5881 gccatagcat gtacatttcc aagacctgca ttactaaagg caattccatt taaaaaacaa
     5941 gcataacaca tttgttctct agcttctatg tcattaccat ttttaacagc tcttggaagg
     6001 tatttaaata tctgctttat agcatataag gcagttgaat ctgtaacatc ataagcacca
     6061 ttagctacta cagcttctac agcatgagtt agtgcatcca taccagttgc tgctgttaaa
     6121 ttagatggtt tgctaatcat taattctggg tcatttactg tcattgtagc tagtgagttg
     6181 gtatcaacca ttatcatctt tacatgtctg tcttcatcag ttataacata gtttattgta
     6241 acttcagcag atgtaccagc agtagtagtt atagctacga taggaagaca ttttttagaa
     6301 gttttgttga taccttcata gtctctaata tctcctccat tagtagctaa tatagagata
     6361 gctttaccac agtcttgtgg agaacctcct cctatagtta taacgaagtc acaattttct
     6421 gattttaata ttttaagtcc tttatccaca ttagttacgg ttggattttg tttaacttca
     6481 tcataaacaa caaagtctac atctatctga gataaaatat ctgttacttt ttttacagta
     6541 ccatttgaaa ctagaaattt atcactaaca acaaatgctt ttttagttcc taatgattta
     6601 attgaatctt tagcttcatt aagacatcct ttcccaagta aatttattgg tggtacataa
     6661 taagtgctca taactaaaat ctccttttat tttcattgat ttttatgtaa aaatatatat
     6721 ctaacaattt tcactaattt actcataata tatcgtatct tagattgaga tagggttact
     6781 ggttcttata taagaactta attcgtaatt acgattatga ttagaattta acatagtatt
     6841 tttatcatgt caataaaaat ataaaaaata ttataaaaaa aacaaagtta ctgtagttaa
     6901 atcagcaact ttgtttttat agtataaatc aaataaatac attagtttaa aacaaaattt
     6961 ttaattatta tgttttgata tcttccatat acagaatatt aatctaattt attacaatgt
     7021 cttcattacg ttcttccatc attgattgaa tatttttctg agcatttaag agtaattttt
     7081 ttatcagttc tattttttcg tcattagctc taaataaagg cagtgttaca ctaacagcag
     7141 ctcttataca tccatttttt cttattggta cggcaagaca ttttaggtgt tccattgatt
     7201 cttcacactc tgaagctatt tgtgttcttt gaacttcaag aagctggtcg tataaaacat
     7261 caaaatctgt aattgatttt tctgtaaaac cttttagacc atctggatat aagcttatta
     7321 gtttttcctt gtcaaaatca cataaaagag cttttccaag acctgtacaa ttagcaggta
     7381 ttttttttcc aacataagaa ataagtctaa ttggttctgg agaatctact tttccaatat
     7441 aaagaacttc attattgtca agtataccaa gctgacaagt ttcagaacag gaattaacta
     7501 tattagtcat ttcgtcaatt atatagttaa aaatagttct tttttcaaaa aatgaagacc
     7561 ctaatgtgta agaacgtatc cctatagaat atttggatgt gtttttatca atagtaatgt
     7621 attttctagc attaagtgta tgaactatag gaaagatgct actttttgga gcattgataa
     7681 catctgctat ttctgtaaga gtatatccat tttcggattc tgctaatgct tctaaaatat
     7741 ctaatatcct agctgttggg cgatgattat ctgaagtcat aaatttcccc ctctttttat
     7801 taatttaaag tattagaata tgttgatttt agcacactat acgatatttt acaatgtaga
     7861 aaataaatat gtaaatttat atataaataa gctcttgatt tagatacaga tagatatttt
     7921 tttagactgt acattaagga ataactaatt tccaaaatat acctaaagaa agtaagtgat
     7981 atttagttat ataatttaag taaatatatg caatagttat ttttatatgt aaatatcaaa
     8041 atattatgaa tttatatttt atctattttt tacactgtat aaaaacttta tagttttgaa
     8101 tactatataa ttttaaatac gctgtatttt tgcatataag gaagtcattt gataatatac
     8161 aaggtattat cagaaagttt gaaaaaaatt tgacaaaaat ttattttttt tattgactca
     8221 taaataggaa aatgctatca ttaattcaaa gattcgtaat taaaaactta gttcttaaat
     8281 acgaaccgaa acggccgtta gaattaaagc taaaaactta ataagagtaa ttaaatatca
     8341 tgttttagga ggattaactt tatgataatg gattttatta aaaaaattcc tgcaggtatg
     8401 atgatagtgc caatgtttat agcagctttc attaatacct tttgcccaga tattgtacaa
     8461 ataggttcgt ttacgacagc agtattttca agtgttgggt ctgcaactat aattggggtt
     8521 caactggtat gcttgggaac tcagcttcaa tttagagaaa tgccaaaagt tttaaagaga
     8581 ggtggtatac ttcttggttc taaatttgct ataggtgctt tgataggtat acttatagga
     8641 aagctatttg gaatggatgg tgtccttggt cttacaacat tggctgttat aagtgcagtt
     8701 acaaatagta atggtagtat atatctttca ttgatgactt catatggaga tgaaacagat
     8761 tgtgcaacta tgagtttact tactttaaat gatggaccat tttttacatt aatagctctt
     8821 gggacttcag gtcttgcaaa tgtgccattg ttgtcattat tagctgcaat agtgccaata
     8881 cttgttggta tgataatagg aaattttgac aaaaaaatga aagagttttt agagccaggg
     8941 tgtaatttac tcgtaccttt tgtagggttt gcacttggta gtagcataaa tcttgcaagt
     9001 atactaaaag gaggtttatc aggagtatta cttggattaa tatctgtatt tgtaggaggt
     9061 atatttatag tattctgtga taaatttata ggaaaacgtc caggatatgc aggatgggct
     9121 gtagctacaa ccgctggaaa tgcaattgct gtacctgctg ctgttgcatt agttgaccca
     9181 tcatggcaac catatgttgc aacagctaca acacaagtgg cagccgcagt agttgtcact
     9241 gcaatattag tacctcttat aactagttgg tgggctaaga agtatggatg tcctaagttc
     9301 ccaaaacaaa atgaggatgg tacaataaca gcttaattta tattacagtg agttgttggt
     9361 aaaataataa attctttaat tagattttag gatattaata aaattatatt atttataaag
     9421 gagagcaaga ataatgataa atgcagtgat aattgatgaa aaagataatg ttgcagtagc
     9481 tattgaagca atagctaaag gtaatgaaat tagttttaaa ttaaaagaca aaacagtaaa
     9541 aacgattaca gcactagata atataactat ttatcataaa ttggctataa aagatatggc
     9601 ccaaggtgat aaagttacta aatatggtga acatatagga gaagcaaatc aggaaataaa
     9661 aagtggtcaa catgtacata ttcataatgt tagaagtgtt agagaggatt tggataaaca
     9721 agttatgact aatatataaa ataatattta tagagttata aaagttgtat tcattgcttt
     9781 tagtgtcaaa tagtttttaa ataggatttt agctgtttga ttagtgagtg taataaaaat
     9841 tataatatat aaaaattata aaaagtatat agactatttt aggaggaaat cagtatgaaa
     9901 ttttatggat ataaaagacc agatggaaga gttggtataa gaaaccacat attaatattg
     9961 cctacgagtg tgtgtgcatc agatacaact agaataatag catcacaagt tcaaggggca
    10021 gttaccttta ataatcagaa tggatgttca caagtacatt cagaccaaca attaactatg
    10081 gatgttatgg ctggttatgc agcaaatcca aatgtatatg gtgtaatagt tgtatctttg
    10141 ggatgcgaaa attgccaaaa tgatttggta gttgatgcaa taaaagagag aacaaataag
    10201 ccaataaaaa ctcttgtaat acaagaagaa catggaactt taaaaactat agaaaaagct
    10261 gttcgttatg ctagagaaat ggcacaagaa gcttctttgc ttagaaaaga agaatttcca
    10321 atatctgaac ttatactagg aactgaatgt ggtgggtctg acccaacaag tggattagca
    10381 gcaaatccat taataggtga attaagtgat aaacttgtcg atttaggagc aacatcaatt
    10441 ttaagtgaaa ctactgaatt tataggtgca gagcatatac tagctaggag agcagtaaat
    10501 gaagaagtaa aagaaaaaat attacatata gtacatagat atgaaaattc attaaaattg
    10561 gttggagaag aagttagaga aggtaatcca tctcctggaa atatagctgg tggtttaaca
    10621 agtttagaag aaaaatcact tggttgtata cataaaggag gtcatagaca aataagtgaa
    10681 gtatatgatt atgccaaaca aattgataag aaaggtcttg ttataatgga tacccctgga
    10741 aatgatgctt catctgttgc tggtatggta gcaggaggag cacaaatagt ggtatttagt
    10801 acaggaagag gaacaccatc aggaaatcca atagcaccag taataaaaat aactggaaat
    10861 aaaataactt ttgcaaatat ggaagataat atggattttg atgctagtcc agttatctat
    10921 ggtccacaaa caatggaaga attaacagat gatttgttaa atatggttgt agatgtagca
    10981 aatggaaaac aatcaaaagc agagtccctt ggatatactg aaatggcaat agctagagta
    11041 tgcaactatg tataatcttt aagtaagaaa ataaatacat agaattttaa taaagggtga
    11101 gattatgaag tttgtaagtt ttactgaagg agaaaatgaa tgtaaaagaa ctggagtttt
    11161 ttctaaagat gaattattta ttatagatgt taactcttta aatctcagta gaaattttaa
    11221 ggatttaaat gaattaattc aaaatgtatc tagtgaagat attattaagt tgaaaactat
    11281 tataaatgaa agtatattct ataattatga tgtttttaaa agagagagat gtactgtaca
    11341 tgttcctata gaaaaaccaa tacatgatat cttatgtgtg ggagtgaact ataaagacca
    11401 tcttgaagaa actcaaactc actttgatga tagttttgaa gagccacaaa aaacggttta
    11461 ttttacaaaa cgtgtgacaa aggcaatagg tccagaagat gagatagatg gtcattttga
    11521 gataaataat caactagact atgaagttga acttggagta gtaataggta aaacaggtgt
    11581 taatattaga aaagaagagg tagaagatta tatctttggt tacactgtta taaatgatat
    11641 ttctgctaga aagttgcaaa aagaacatgt tcaatggttt agaggaaaag gattagatac
    11701 atttacctct ttaggtccat gtattttaca taaaagtgca gttccttttc ccataaaact
    11761 taaagtatgt agtagggtaa atggtgaaga aagacaaagt tctaatacag gattattcat
    11821 atcagatgtt tcagacatag tttcagaact ttcaaaaggt atgactttag aggctggaga
    11881 cattatagct actggaacac cttctggagt agggatggga ttttcacctc ctcgatatat
    11941 gagtagtgga gatgtagttg aatgtgagat tgagagtatt ggaattttaa aaaattacat
    12001 taagtaaatg ttttaatata attaatatta aagctaaatc tgatgtatta atgaacatat
    12061 taaaataaat tttaggagtt taaattatga aagcagaatt tttgacacca gttgttacaa
    12121 tatttgatga agaaggaaat ttagataaag aaggtaataa aaaagtgtac gaccatctta
    12181 tagatggagg tgtagatgga ctagtcataa tgggaagtac tggagagttc tttaatatgt
    12241 ctatggatat gcaaaaagaa cttatagatt tagtaactag tcatgtaaat aagagagtaa
    12301 aagtgtttat aggtacaagt cgtatgtgta taaatgaatc tatagaactt tcaaactatg
    12361 cacataatag tggagcagat ggcgttatga taataagccc ttattatttc tctcttgatg
    12421 atgatagtat tgagttattt tatagtgaaa tagctaagaa tacagaggct gaaatatatt
    12481 tatacaatta tccagataga actggttatg atttaagtcc agaacttaca ttaagacttg
    12541 caagaaaaca taaaaacata gttggatata aggatacagt aactttgatg ggacatacca
    12601 gagatttaat aaatactatg aaaatagagt tcccagaatt taaggtttat agtggttatg
    12661 atgaaaactt tatacataat ctaatgtctg ggggagcagg ttgtataggg ggattatcaa
    12721 atttaattcc agaagtttgc tccaaatggg ctgaagctta caataataga gatatggaga
    12781 aggttataga aattcaaaaa tatattaata aagcaatgga tttttactta ataaccaatc
    12841 catttatacc agcaatgaaa aaagctatga atatcagagg gttacaaata agtgattata
    12901 tgactgctcc atttgtaaaa ccaaatgaaa aacaagtatg tgaaattaaa aaattgatga
    12961 ataaattaaa tatatgttaa atcaaccttg caaagaggtc aacaataaat tacaattatc
    13021 ttaataaaaa ttaaattaaa ataatactag ggtactatat tagtttaata gggaaagtga
    13081 tgaaaattca ctacagcccc cgctactgta tacggatacg aaatcaaaat ccactgaaat
    13141 ttataaaaat tttgggaagg gtgatggagg aaaagccgtg agtcaggaga cctgcccagt
    13201 attatataag ttcaatttag ggtggagatt gtaatttata ataaaatgaa gtatcaattc
    13261 gtttttgtaa agttgatata tgaacttctc cttattaaat taggagaagt tttttatttt
    13321 attaaaaggt tatacttatt aaagatgact ttaggataac tttaataagt atactttaaa
    13381 aagcatatat tattaaagtt ttttagccac tcataaaaaa taagatattg gagtgaaaga
    13441 gatgaaattt aaatcaaatg ttttaaagca gtttttttta attgctatgg taatttttat
    13501 ggtaggaggt cttatagggt gtagtaaaag tgacaaagag aataaacaag ctagtactgg
    13561 taagaaatta gaattaaaat atgctagtgg atttagtgtt gaagatttag aagatggaat
    13621 taaaaaagta actgatggag ataatcgaga actgatttta atcccaaaga agattaaaat
    13681 tcctgaaaaa tataaaaatg ctaatatagt tagaacgcct gtggacaatg tattaatagc
    13741 atctacaaca caggcttgtt ctctgagaac aatagatgaa ctagatagta taaaagccgt
    13801 aacaacagaa gagcagtact ggactattaa agaaattaaa gatttaatga aagatggaaa
    13861 aatacattac gttggttcaa atttagcacc agattatgaa aaaatagtag acttaaatcc
    13921 tgacttaact atagtgagta gtggtttatc agaggctgat acaaaattta ttgacaaatt
    13981 aaaagaatta gggcttaatt atgttgtaga caatgagtat aaagagcaaa atccatttgg
    14041 aagaatggag tggacaagtt taatagctgc attttatgat aaagaagata tggctactag
    14101 tgaactaaat aaaactgtac aaaaggtaga agaagtaagt aaaaaaataa aaaacaaagc
    14161 taaacctaaa gttgtatggg catctgtata tgaaggaagt gcttatatag ctcctaaaga
    14221 ttcatatgtg gataatatga taaaaatggc tggtggaata aatgcatgtg cttcaataga
    14281 ttcaaatgaa gggcgagtaa gtattgaaca attgcatgaa gttgcaaaag atgcagatat
    14341 atttgtatac tcatcaagtt cagattatgc accaaattta gattctataa aatctagtgc
    14401 acctgtattg gctgatttgg atgttgtaaa aaacggaaac ctatgggtgt ttgctccaga
    14461 ttattatcaa tcagtagata agacggacga attaatagta gatttagttg agatgttcca
    14521 cccaggtact acaggtgaaa aaatcaaaca ttatataaat tatgataaat agtacagaga
    14581 ggacacaaaa agataggttt tatagatttt agttaatata actttgctaa gaggagtata
    14641 caaatggatt ttatcaataa aatgagaata aggaatacga caatatttat agtattaatt
    14701 ataagtatga tacttacttt tttagtgtgt gtagcatttg gctctgtaaa aattgaattt
    14761 aaagaagtaa tagatgttgt attaagaggt atggataata cagaacatta taaaataata
    14821 tacaatataa gaatccctag agtacttgca accatggtag gtggagcatg cctagcagta
    14881 tcaggtattt tattacagat attttttaaa aacccaatag ttgaaccgta tatattagga
    14941 gtatcttcag gttctacttt atcagtagct ctcatgattt tgggaggata cacattggga
    15001 tttgagagtg taaattcaat gagtatattt gtagcagctt ttataggagc tttaattgta
    15061 atgtctatag tcattttatt tgcaaataag gtaaagaata taacaactct tttaataata
    15121 ggaattatga ccggatatgt atgtagtggt ataacaaatg tgttacaatc atttgcaaat
    15181 agtgataaat taaaagaatt tactatgtgg tctatgggaa gtttttctgg atttacatgg
    15241 gaaaaggtag aaatacttat aatagttggt atattaggtc ttttgatgac tgtattcata
    15301 ataaaacctt taaatgcttt tttattagga gaagagtatg caaaaagtat tggcataaat
    15361 ataagatttt ttagagtttt gatagtgttt atatctagta tattagcagc aatagtatca
    15421 gcatttgcag gacctatagg gtttattgga cttgcagtgc cacaaatttc aaaattgatt
    15481 aaaaaatagc ta
//
LOCUS       DAMKPG010000086        15464 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMKPG010000086 DAMKPG010000000
VERSION     DAMKPG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.090"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            170..1561
                     /locus_tag="PC621_002853"
     CDS             170..1561
                     /locus_tag="PC621_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF4109999.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1814..3607
                     /locus_tag="PC621_002854"
     CDS             1814..3607
                     /locus_tag="PC621_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4110000.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3688..5070)
                     /locus_tag="PC621_002855"
     CDS             complement(3688..5070)
                     /locus_tag="PC621_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF4110001.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5328..5675
                     /locus_tag="PC621_002856"
     CDS             5328..5675
                     /locus_tag="PC621_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF4110002.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5693..6727
                     /locus_tag="PC621_002857"
     CDS             5693..6727
                     /locus_tag="PC621_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110003.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6776..7825)
                     /locus_tag="PC621_002858"
     CDS             complement(6776..7825)
                     /locus_tag="PC621_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4110004.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8100..9635
                     /locus_tag="PC621_002859"
     CDS             8100..9635
                     /locus_tag="PC621_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110005.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9821..10507)
                     /locus_tag="PC621_002860"
     CDS             complement(9821..10507)
                     /locus_tag="PC621_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF4110006.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10632..12014
                     /locus_tag="PC621_002861"
     CDS             10632..12014
                     /locus_tag="PC621_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF4110007.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12416..13684
                     /gene="eam"
                     /locus_tag="PC621_002862"
     CDS             12416..13684
                     /gene="eam"
                     /locus_tag="PC621_002862"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF4110008.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13777..14133)
                     /locus_tag="PC621_002863"
     CDS             complement(13777..14133)
                     /locus_tag="PC621_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110009.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14284..15378)
                     /locus_tag="PC621_002864"
     CDS             complement(14284..15378)
                     /locus_tag="PC621_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF4110010.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5990 a         1913 c         2488 g         5073 t
ORIGIN      
        1 taaaaaaata atatttttca taattttaaa aataataata aaaaaatgaa aatacaggcg
       61 ataatctatt gagtaaacat agaaaagatg atattataag tatgtgctaa aatggttaaa
      121 gcaatattaa tattaaattc aactataaaa caaaaggggg cgttttttta tgtcaagtac
      181 agaaagcgta tctaaaaaaa gtggtcatcc gaagagtttt tggcttattt gcatgacaat
      241 tttgtgggaa agatttagtt attatggttt aacagcttta ttggtattat tttttacagc
      301 aagtgtagct caaggtggtt taggtctttc tactcaagaa gcaacatcca tgtttggctt
      361 atttacaggt cttatatatc ttacaccatt agtcggtggc tggttagcag ataaagtttt
      421 aggtcaacaa aaatgtattt ctataggtgc acttttaata gcaataggag atttcgtact
      481 atttgcttca tctacatcta ttgccatggt ttgggtaggt ctatcaataa ttattttagg
      541 aaatggtttt tttaaatcaa gttgttcaaa tatagttggg gatatatatc ctaaaactga
      601 tgtgtcaaga aaagatgcag catacagttt attctatatg gcagtaaatg taggagcatt
      661 tatagctcca ataatatgtg gattgctttc tgataatgta tttgcagtaa gaggagcaag
      721 tggagatata gtatcttatg gatataagat ggcattttta atttgtggta taggcatgat
      781 gttaggtatg attatattta cacttcttgc accaaaatta ttaaatgaag tcggaaaata
      841 tcctgtgact aaaggtaata aaggtgaaaa aattgagtat ggaccactta ctaagttgga
      901 gaaaaatagg ataatagcaa tggttatttt atttttattt gtggtcttat tctggacagc
      961 atactatcaa acacaaagtt cttttatgat ttatgtaagg gataatgtaa atagaactct
     1021 atttggattt gaaatgccag tagcatggct tacatcacta aatgctatat tatgtgtggc
     1081 tttatgccca gtattagcta ccctatgggt taaattatct cataccaaaa gaggtgattt
     1141 ctctattcca gttaaaatgg gattaggaat gataataatg ggtgttgggt ttatagttat
     1201 ggtatttgct acattagcta atggtccaga tggaatggta aaggctagta tattgtttat
     1261 agtattgact tatatattta caacagtagg tgaattattc ttatctccta taggacttgc
     1321 aatgttcaat aaattagctc cagctaagta tgctacatta tctatgggag cctggtatct
     1381 aacaagtttt tttgcaagtt tattatcagg aaagattgct ggttttacag ccacacttgg
     1441 ttttttacag atatttggag gaatagcaac agttttaata atatttggtt taatattatt
     1501 gggaataaga aatgtattaa caagattaat ggctatggat ttactaacaa aagaaaaata
     1561 attcatgacg aattattgta aatagatgaa ttacttcaaa taaaggaagt tacatttaaa
     1621 tataaaatta aatgtaattt cctctttgat tatgaaaaaa agctttacac aaatagatat
     1681 tataattaaa attataattg tgataattag agtgtataag atttatataa attatacaaa
     1741 aaatatttat gtaataaact ttacatactt atatatttag ttattcaaac tattatttcg
     1801 aggaggtctt gttgtgaata taaaagatag attaagtgga ttaagaaaat ttatggaaga
     1861 aaaaaatata gatgcctata tgattccatc ttctgataat caccaaagtg aatatgtagg
     1921 agattacttc aaatcaagag aatttatatc agggtttaat ggttcagcag ggacggttat
     1981 tgtgactaaa gacgaagctg ggttatggac cgatggaaga tattttatac aggcagaatc
     2041 tcaattagaa ggaagtacaa taaaattatt caaaatggga caagaaggtt gcccaactac
     2101 agatgaatat ttatataaaa atattcctga aggaggaact ttaggatttg atggtagagt
     2161 tatatctgct agagaaggag ctacattagc tgaaaaatta tctaaaaaag gtataaagat
     2221 agaatatcag tatgatttaa tagatggtat atggccagat agacctgctt tatcagatag
     2281 taaagcattt ttattagatg ttaaatattg tggagaaagt tttagctcaa aattagctag
     2341 attaagagaa aaaatgagtg aaaaaggtac ttctactcat gtaataacta ctcttgatga
     2401 tatagcatgg ttattcaata taagaggtgg agatgttaaa tataatccag ttgtactttc
     2461 ttatgcagta ataactttaa aagaagttta tttatttgtt gatgaaagta agttaaatga
     2521 agaaatatta gatgaactag ctaaggaaaa tgttcaaata aagccatata atgatgtgta
     2581 tgaatttgta aaaaatatag acaaaactga aaaagtttta ttagatggaa ctaagcttag
     2641 ttatacaatt tacaataata taccttgtga agttgagaaa gttgatgagt tcaatccagt
     2701 aatgtttttt aaagctcaaa aaaatgaggt agaattagaa aatataagaa attcacatgt
     2761 aaaagatggt gtggctttca ctaaatttat gtactggtta aagaaaaatg taggtaagat
     2821 ggaaatcact gaaataagtg ctactcaaaa attagaggac ttaagaagag agcaagaagg
     2881 tttctttgag ccaagtttca atacaattgc tgcatataaa gagcatgctg caatgatgca
     2941 ctatagtgca actccagagt caaattataa attagaggca gaaggattat ttttagtaga
     3001 ttcaggtgga caatattatg atggaactac tgatataact aggactacag ttttaggacc
     3061 tataagtgat gaattaaaac tacactttac ttctgtagca agaggaatga taaatctttc
     3121 taaggctaag ttcttgcatg gttgtagagg atataactta gacatactat ctagaagttg
     3181 catgtggaat atgggaattg attatcaatg tggaactgga catggtatag gatttgtgct
     3241 aaatgttcac gaagctccaa atggatttag atggagagta gtacctgaaa gatttgatag
     3301 tgcagttctt gaagaaggaa tggtaactac taatgaacca ggtatatata tagaaggttc
     3361 tcatggtata agaacagaga atgaaattgt agttagaaaa gctgaaaaga atttctatgg
     3421 tcaatttatg gaatttgaag ttgttacgtt agctcctatt gacttagatg gtatagtacc
     3481 agaattaatg aataaggatg aaaaagatta tttaaactgg tatcataagt tagtttatga
     3541 taaaatatca cctttcttaa ctgatgaaga aagagaatgg ttaaaagttt acactagagc
     3601 tatatagttt gtactagata caataaaaat cgaggtatta taatgcctcg attttttatt
     3661 gtatatttta ttaactttaa aaaattttta atttgataat tctactaatt ttttattcat
     3721 tataagtagt ataattccaa atatcaataa aaatgatggt atcactatga agacttgtaa
     3781 cataccaagt tggtctataa tagcttgcgt atagccagat aatttatttg ctgcaaattt
     3841 gcctaaaaac caaacaccca ttaggaatga aatatacttt tcaggcgaaa gtttatttac
     3901 cataccatac ccgactggag aaaagcaaat ttctcctata ctttggaatg ttaaaaatcc
     3961 aactacccaa aatacacttg ctttgtgaat tgaattaggg tctgttcctg tttgtataac
     4021 tgctactatc ataaacaaaa atgctatagc taatagtata aaacctaagc tcattttttt
     4081 tggcacactt aaatcgcctt ttttagtttg agataatttt acccatacta aagcagagat
     4141 aggtccaaga ataacacaaa ggagaccatt gtatgaatct atccatgaag taggaatttg
     4201 aaaattgcca acagtaagat ttatgtgttc ctttgtatag agtgcaatag acatagaggc
     4261 ttggttatat gctatccaaa aaaatactgt gaaaaaagct aacaccaata ttactattat
     4321 tctttttttc tcaatcaaag ttaaaggtct tgaagcgagc attttcttat attctagtga
     4381 ttttcttata gactctctcc cgttactagg gtcattattc aaaggtttat aaaactttct
     4441 gtttccaaat ataaagaaga ataaacctat tagaaccgta attgatgaaa ataaaaatac
     4501 atatctatat ccatacatac ttatagataa tccgcttttc atagcaaacc attcattagc
     4561 aactaaaccg catattaaag ggccaaataa agtacctaga ttagtagcca tatacgtaat
     4621 agagaaagct ccatcttttc ttgggtcatc attggtatat aagtttccaa ccatagtatg
     4681 taagtttcct ttataaaaac ctattcctaa tccagataaa aatatcatga tgtttatatc
     4741 catcttacta tttgctatgt atcctagaaa ataaccaatt gaaaccatta gcattccaag
     4801 tataatacag tttttgggac ctaaaaatct atcactgacc catccaccaa taactggaga
     4861 gaaataagtc catgctaaaa aagatgcaag cattgatgcg gattctgctg gtgttaaccc
     4921 taaaccacct ttggagactt gttcagctaa aaacaataat aataccaatt tcataccata
     4981 gtatgcaagt cctacaaagg taaatattga acagcagaaa tataaatctc taggttgttt
     5041 aagtttttct tgcgttttta aaactgacat atgtcgagaa cctccagata aatagtttaa
     5101 caaaatttta ccatactata tgagtatatg tttttttcat atagacgtca acttgtaaga
     5161 taatttaatt aagtcgaaat atatgtaaaa atagtatcat ttttgtgtat tcagtaatat
     5221 ttttttatga taatatatgt tattattata ttaaaataca aacatattta tggaaaggat
     5281 atgccaattt agtagttaga ttgattgttt ggcaggtggg atgagctatg ataagaaaga
     5341 aatctgaatt acaagtagaa gtaaaagaaa atttaaatgg tggtatagga aaggttaagc
     5401 ttgaaaacat aatacaaaat gaagagctta aaggaaaagg aagactattt aaaagagtag
     5461 ctttaccagt aggaagttca attggtgtgc atgaccatac aacagatttt gaagtatatt
     5521 atatactaaa gggaaaaggt aaagtatttg ataatgggga atttgtagaa gtaaatgagg
     5581 gtgatgtagt gtatacagct gatggcgaaa aacattctat agaaaatata ggagaagaag
     5641 atcttgagtt tgtagcacta gttttatata tgtaaaatat ggaggaggag tcatgacagg
     5701 caagatagaa gaaattaagg acaagataaa tagattttgc aactccaatt taaatcaaga
     5761 atacaaagat ataagcttta aaattttgca aaacctttta gataataata aagagataat
     5821 tcattcttct agagctgata tatggtcttc agctatacta aatatagttt tagaacaaaa
     5881 tcttttatac aataagaagc atccactaca tataacaaaa aaagagtttt caaaacaaat
     5941 aggtgtttct ttaaatacta taaataataa atcgtcttta ataaaagatg tttgtagtat
     6001 tgacttttta aatcaagata ctatagctat ttctaatgta gaattttggg taaaagagac
     6061 caattccaaa tcaaagtctg tagatttaga tttagaaaag aaaaaaatac tatataaaag
     6121 atatattaag ctatcacaag atgcgaatag tgatattgaa tcaattagat atatggagga
     6181 agctgtaaat ataggtaaaa gtatgattac taaagaagat aaagaacttg gattttggaa
     6241 aggtttatct accagagcct atatgatagc acttgaatcc cttgctagaa agttagaaag
     6301 tattaataat ctaaaggagt ccaaaaaagt attagaatat ttaatagaaa ttaatcctag
     6361 tgatgagcag ggaataagat acaaattatt aaatgtatta attagattaa atgatagaac
     6421 ttcaataaat aaattatttg aactttataa agaagaaaaa tctgctacat ggatgtacag
     6481 taaagctctt tattacttta aaaataaaag tatgtttttg gcaaatgatt ctattaaggt
     6541 tgctagaagt aaaaataagt acgttggatt atatttgtta gattggcgaa atgcttttgg
     6601 tagagaattt gttacagagg aagaaaaggc agaagctgtc tattactatg atgaaaacat
     6661 agtggtttgg aatgaagtta aaggttctat ggattggcta ttaaaaaaaa tgttggaatt
     6721 ttcataaaaa aaactactcc ttacttttat ataagtaaaa ggagtagttt ttaatttata
     6781 catctagttt taaatcttca ggttttatta gacctttttt tctcataaat tctgaaatca
     6841 acaaagttac aatagcagga agtataaaat taagtaaaag tataccaacc cacatagata
     6901 tacctccttt tccagcactt tgcatagcag ttactgttcc aaattgtcca accaatccac
     6961 aagtacccat accagaacca ataggaatat tctccatttt gaatattgtt gtagataatg
     7021 gtccaagtaa tattgaagct acagtaggtg gtatccaaat tttccaattt tttattatat
     7081 taggtacttg caacatagat gtcccaagac cttgtgccac aaggccacca actctatttt
     7141 ctctaaagct cataacagca aagcctacca tttggcaaca acaacctaca gttgctgcac
     7201 caccagcaag accacctaaa cttaacatca tacaaatagc tgcactactt ataggtaatg
     7261 taagagctat tccaacaagt gcagagacta aagctcccat tattataggt tgcatagttg
     7321 tagcttgcat tattatattt ccaaatgaag tcataaaagc agaaacagct ggaccaataa
     7381 gttcagctac aagtactcca attagtattg tcacagcagg tgtaaccaat atatctattt
     7441 ttgtttcttt tgaaactaat tttccaacct ctacagcaaa aagtgtagcg acaaatgctc
     7501 caacaggtcc tcctaattca ttaccacaag ctccaacaag tgcacaagaa aatagtacaa
     7561 gtggaggtgc ttgaagtcca tacgcaatag ccactgctat agctggacca gtaacctgtt
     7621 tagcaagagg gcttataact tcagtaaaaa gagaaatatt gaaattatct cctaatgtat
     7681 tgaaaattgt tccaattaaa agggacgaaa aaagaccaag tgccatataa cttagtgcgt
     7741 caataagata cctttgaaca gaaatttcaa tattttttct tttagcaaat gatttgaaat
     7801 ttcttttttt ataattgatt tccaatttaa aatcctcctc atataatata agtataattt
     7861 ctttttaatt aattatataa cttttcagct gacaagacaa atgttatttc actaaaatta
     7921 gactattgta atagtaagaa attaacatta attttagttt taaattttaa atttatggtt
     7981 tataattagt atagggatat tctgaaatat aataattttc cagtttatcc caatatttaa
     8041 cttaggtaga ttgcttacaa aacaaatgat tatatattag gctaaaagga agtggataga
     8101 tgatatttgt aaaaaataag aaatacagaa aaattgccat aattatatct attcctatat
     8161 tgcttgttgc gcagtactac ctttttaaat ttggaatact tagaccaatg gtaaagggag
     8221 tagaaattga aattgtagat ggggaatatg ttaaagatat agataaatat gtagtaaaat
     8281 taaatgaaac agttactttg tctacaggtg aatatattaa ggttccttcg tatgctaaaa
     8341 atccaaatat ttggtttaat gtattggata actcagggac tttaaaaata gatggaaata
     8401 aaatgacagc attaaaggtt gggtattctt ctgtgggtat catgaaaaat tcaagagtat
     8461 taaaaaaggc tactataaaa gtagtagacc cagaggtaga atcactagat atggagatta
     8521 atggggattt aaaacatgtt ggtgatagtg ctactataga aagtacaata gaagttgatt
     8581 atgataagtt taaaaaatca tataagccag tttataagag tagcaatgaa aatattttaa
     8641 aaatcaaagg aaataaagta agtgcagttg gagttggtaa agctacaata tatgctaagt
     8701 gtggaaataa agaaattgag agaacattta ggattcaagc tagagttgct gaaataaata
     8761 tggatgatat aaaaatagaa gttgaccaaa atgaaaaatt gaaaccagat ataataactt
     8821 ctccacctgg tttagaacca ccaaaaatat attatgagtt agtagattca aaattacctg
     8881 tagaaagagc aataagttta agtagtaatg gaaatgttgt aggtcttaga gaaggtaaag
     8941 atagggtgag aataacttgt ggagataaat ctaaagtaat aacagtaaca gtagtcaaag
     9001 aaactataac gaatgactat atacaaaact tggttggttc acataaaata gtaaacaata
     9061 aagttattat taaacttgaa tgggattata tgaaagatgt ctttgattat gatatatatc
     9121 ttaaagataa gttgtcagga gaatcattta agaaaattaa atctataact ataaatgaaa
     9181 gtgatttagg taagtctaac aaggtatatg caactataga agtagatatg aagggtaatc
     9241 aaaatataga ttttgatatt tatgtcatag ggaaaaagga ttcagataca actaagccaa
     9301 gtaatgtaat aaatattact aatggaaatg gaaattcaaa taatgataat aatattaaag
     9361 atatgagggt agaaaatata tcagctaatg ttgatagaga taataatgtc attagagtta
     9421 actggagtag tatatctcat aaggactgta cttacagtat atatgtaaaa aataatacta
     9481 atggagatgg agaatttaat ctatatcaaa gtgatataca gggaaatgag catactataa
     9541 gtattcctga aggagattta aatctagata tttatgttgt tgcaaattat aatggaaata
     9601 gctctcaaaa tagtgatgtt ataaatgtaa gatagatttg tatacaaatt gaaagtgttg
     9661 agtttattta aatattaaat taagaaaaag gatagtatag ggtaattata ctatcctttt
     9721 tttgttacaa tatataggtt taaaatctct agtaatataa attataaaaa gtcttattta
     9781 cacaaatcta tagatatttt ttttattaat caatattgtc ttaaattgaa cttagtgcgg
     9841 cttctattgc atgtattact gtcgtatcaa atagtggaat agaagtgtca ttttgtttaa
     9901 ttaaaagacc tatttctgta catcctaata taacaccctc tgcaccttgt ttgccaagct
     9961 tatctataat agataagaaa gctttttttg aagattcaga tataattcct aagcaaagtt
    10021 cgttaaatat tgtgtcattt actattatac ggtcttcttc atttggaatt aagacttcaa
    10081 ttccattatt aataattaca tttttataaa aatcttgttc cattgtatat ttagtaccta
    10141 ataaggccac tttttttatt tctttttccc ttaatactgt agcagttaca tctgctatgt
    10201 gaagtaatgg aatatgtata ctttcttgta ttttatctga taccttatgc atagtattag
    10261 tacagataac aataaaatct gcccctgcct cttgtaattt aattgcagca tcagctaaaa
    10321 ttttggcgct tttttcccaa tttccactag attggcattc ttcaatttct tgaaaatcta
    10381 cactgtaaag tatacattta gaagagtgta gtccacctaa tctttctttt attactgtat
    10441 taataacttg ataatatgta atggtacttt cccaactcat accaccaatt aagcctatag
    10501 tcttcatatt atacctctct tatttattta ttttgaaata gctatttaca aatacaagta
    10561 tagcatctaa aatagaagca tacaataata aaattgtatg gagtacaatt ataattaaga
    10621 ggagtaatta tatgccaata aattcttttg aaaactatcc tatgacatgg aaaccaaaat
    10681 tagataaccg aaaaccacca atatataaaa ctttagcaat gttattagaa gaagatatta
    10741 aaagaggaaa tttaaatcct ggagataaac tgcctccaca acgggaatta gcagattttc
    10801 tagatttaaa cttaagtaca ataacacgag catttaaact ttgcgaagaa aagggactaa
    10861 tatgtgcaaa agtgggaaag ggaacattca tttcttctga tgtaaatgta agtaatacat
    10921 tattatatca aacggaatca aaagacataa ttgaattagg gacagttcat cctccatatg
    10981 agcaaaatac atatattatt gactttatta aaaatgtact aaaacaacca gaaatggata
    11041 ggtttttaca atacatgtct ccatctggca catatatgca aaaaaagtca attgcaaaat
    11101 ggatagaaag aaataatgta tacaccagtg aagaaaatat attattatct acaggagggc
    11161 aaaatgctat atgtgcaact ttactaggat tatttaaagc tggtgataga attgctacag
    11221 attctttaag tttttctgga attaagtcaa tagctaaaat gataggtatt caattagtcc
    11281 caattcctca agaaaataat gaaatatcga ttgaatatct tgaaagttat tgtaaaaatg
    11341 aaaatataaa aggcatatat cttataccag attatcataa tccaacgaca catactatgt
    11401 ctgatttatc tcgtaaaaag atagccaaga tagcaaagca atataatata ataattattg
    11461 aagatgctat aaatagcatt tttagagacg gtatacaaac tcccatattt tctttagcta
    11521 gtgataatac tatatatatt tttagtacat ctaaattttt atgtgctggt cttagagtag
    11581 catttgtagt tgctccaaaa cgatatatag aaaatttaga aaatgcacta tataatatga
    11641 atctaatggt ttctccattt acagcagaaa tagtacatcg tttattatat tctcctatta
    11701 tagataaaat tatagaagag aaaagagatg caattataga aagaaatgaa gttgctgata
    11761 aagttttgtc agattataat ttgattggtg ataagaattg cagttttcgt tggttacttc
    11821 ttcctgatga attggatgga aaatcatttg aaatttctgc aaaaaatcta ggagttcaag
    11881 tatattgtgc tgaacgattt tcagtaggaa actctacagt tccaaaagca gttcgtattt
    11941 gtgtaacagc tccaaaagat gtagaagaat tagaaaaagg gcttaatata ataaagtcac
    12001 ttcttaagag ttaaatttat tttactatta tttaattaaa taaataaaaa ggcggagaaa
    12061 tttattataa taaacctccg cttttattat ttaatgaaat actttacttt ttattaaaaa
    12121 atttaatttt aataatcttg aaaatataat cgttttatgg taaaataatt cttaaaaata
    12181 gaaaaaatta atcatttgtt aaaaaaaatg cataatttat tgtaatttat tgacgaaggt
    12241 tgaaaaatat tcataaatgt gcgaaataac agaaaaaatg caaaaattaa ttcaatttca
    12301 tttgttataa aatctatatg gcaaaattaa atctgcctat cagaatgata taaacacatc
    12361 agaaaggagg acgctatcaa ataatacaag tagttaactt tgatagcaaa aaattatgaa
    12421 tgaacaaact agaatatcct tagagagagc tgctgaatta aaatcaaaaa ttgatgatta
    12481 tattcaggct agaaaaacga ttaacagagg tcttgaaaaa gaagaagaga taaataaacg
    12541 aaaacagaaa atattaagta tcttaaatgg aactgaagag gattggaata actacaaatg
    12601 gcaattatca aatagaataa cagatgtaga tactttatca aaaattataa ctctaactaa
    12661 aaaagaaaaa gaatatataa aagaggttgg tactcaattt agatgggcaa tatctccata
    12721 ttatttgagt cttatagacc cagaagatat atgtgaccca ataaaattac tgtctatacc
    12781 aacacatata gagttggaag atgagcaaga agatttggac ccaatgggag aagagtatac
    12841 aaacccagca ggatgtataa ctagaagata cccagataga ttaataataa atgtaacaaa
    12901 tgagtgtgct atgtattgta gacactgtca gagaagaaga aatattggac aacaagattc
    12961 tcataagtca aaagctatta tccaagaatc tatagactat atcagagaaa atgaagaaat
    13021 aagagatgta ctagtaactg gtggagatgc tcttacatta aaagatgatt atttagagtg
    13081 gattcttagc caacttaaag agataccaca tgttgattat gttagattag gtactaggac
    13141 tcttgttaca atgccacaaa gaattacaga tgaattttgc aatatgctaa aaaaatatca
    13201 ccctgtatat ataaatactc attttaatca tccaatggaa ataactaagg aatctaaaga
    13261 agcttgtgaa aagttagcaa atgcaggagt tccattagga aatcaggcag tattattaaa
    13321 tggaataaat aatgataaat ttgtaatgag atgtttaaat caagaattac taaaaataag
    13381 agtaaaacct tattatatat tccaaagtaa acatgtaaag ggaacaaaac atttcaatac
    13441 atcagtagat gatggtcttg aaatcatgga gtatttaaga ggatatacat caggaatggc
    13501 tataccaaca tatatagtaa atgcgccaaa aggaggagga aagactcctt tgcttccgca
    13561 ataccttgta tcaaaaggaa cagattacgt tatgcttaga acatgggaag gaaaagttat
    13621 aaaaatggaa gatgaacctg ctgtagatat aaagaaactt ataaaagagc aagcacagga
    13681 ttaaaaaagt atttaaagtt tataaaaaga aacaacttta aaataaaaag aaaatggatg
    13741 tcaaatttat atcaatttgc atccattttt tattagttat ttattatttt gttgttgatt
    13801 aactaattca agatatccac ttgggtggaa gtaatatatc tcagaagtat tttcatttac
    13861 aaagtaacca atatcaccat ctgcatctgg taaaaataca taaccattaa gatttttagc
    13921 ttttaatgct tcaaaatcac tagcatttcc ttgatatatg taatcaactt cattgttgta
    13981 ttttactaaa agttctttag cttgttcttg agtcaattta gtattttggc ttacaggaac
    14041 attcttttga gaatctgcat aagaagattc atttactggt gcagttaata acaaacaaga
    14101 taaaactaat aaagatataa gtgctttttt catactaaaa ccctctttca tgtttaatta
    14161 tataagattc attacttaat aatattgtat gtttcctata attaaatatt accattaaag
    14221 agaataatat tacagttaca cagctgtaac ttttgattac aaaatcgtaa ttaaattgta
    14281 tttttattcc atttgtattt tatcatgtct acgatttaca aactcacaga agtatatctt
    14341 tataacgccc atcacaggag cagatagaat cataccaggt ataccaaaga atcctccacc
    14401 aacagtaact gctaggattg taaagaatgg gctaagacct agttgtccac ctacgatttt
    14461 tggctctata aatgtaactt caacttgttg aattattatt aagtaaatta aagaagttaa
    14521 agcaattgtt ggattggaga ataaattaat tattacaact ggtgccatac ctattactgg
    14581 accaaaatat ggaaccatat tcatgacacc cataagtgta ccaaataata gtgcatattc
    14641 agattttaaa aagtaaagac caattccaga aagtactcca actataaaag aatctaaaat
    14701 cttacctgta aagtatttac caatatttaa atttaagctt tgacatagtt ctattataaa
    14761 gtctccatat tttttaccta gggaaatata aactactttt tttgtaaaca gtaagaattt
    14821 ttctttttct aataatatat agaaacaaat tataaaacct aaaagaaatt gtactataaa
    14881 ttttccaact gaaaatgtag tgctaaatat cccaccaagt gaaccaataa gcaagttact
    14941 aaatttaggc attacactca taattttgtc accatactct tttaatgtat tagggtcaac
    15001 tgattgtaaa ctcttaccta agtcgaataa gaactgctct gttttatcta tgtatgtagg
    15061 aatttgagca actaaatcag ctaaactatt tactatggat ggaactgtaa ataatataaa
    15121 ggaaattagt ataactatga gtacaccata agtaagaagt aacgaatata ttctcttaaa
    15181 atttaattta ctttctaaga actttactag tgggttgaat atatatgcta atacaaatgc
    15241 tattataaat ggtgttagta gtgacattaa taaacttatc actccaaaaa aatatttgta
    15301 attatcaata actttaatca agatatatga aacaactaca gtgagtacaa tatttagata
    15361 aattttttta tcttgcaaaa taacacaacc tttcttatat ttattaaatt tagatatact
    15421 taaatctaag ttaaagttaa tatttatagc ttattataca ataa
//
LOCUS       DAMKPG010000087        14928 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMKPG010000087 DAMKPG010000000
VERSION     DAMKPG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.092"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(403..2469)
                     /locus_tag="PC621_002865"
     CDS             complement(403..2469)
                     /locus_tag="PC621_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF4110011.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2546..5422)
                     /locus_tag="PC621_002866"
     CDS             complement(2546..5422)
                     /locus_tag="PC621_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4110012.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5475..6935)
                     /locus_tag="PC621_002867"
     CDS             complement(5475..6935)
                     /locus_tag="PC621_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF4110013.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            complement(7027..7401)
                     /locus_tag="PC621_002868"
     CDS             complement(7027..7401)
                     /locus_tag="PC621_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110014.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7579..8319)
                     /locus_tag="PC621_002869"
     CDS             complement(7579..8319)
                     /locus_tag="PC621_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF4110015.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8340..9128)
                     /locus_tag="PC621_002870"
     CDS             complement(8340..9128)
                     /locus_tag="PC621_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF4110016.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9216..9306)
                     /locus_tag="PC621_002871"
     tRNA            complement(9216..9306)
                     /locus_tag="PC621_002871"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9270..9272),aa:Ser,seq:gct)
     gene            9565..10329
                     /locus_tag="PC621_002872"
     CDS             9565..10329
                     /locus_tag="PC621_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF4110017.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10610..11725)
                     /locus_tag="PC621_002873"
     CDS             complement(10610..11725)
                     /locus_tag="PC621_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF4110018.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11766..13292)
                     /gene="glpK"
                     /locus_tag="PC621_002874"
     CDS             complement(11766..13292)
                     /gene="glpK"
                     /locus_tag="PC621_002874"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF4110019.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13585..14682
                     /locus_tag="PC621_002875"
     CDS             13585..14682
                     /locus_tag="PC621_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF4110020.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         4847 a         2635 c         1596 g         5850 t
ORIGIN      
        1 ttcacattgt tgctttttct taaacaatac taaataatat ctttcacatt gttgcttttc
       61 ttaaattaca ctaaataata tctttcacac tgttgctttt tctttaataa tactaaatag
      121 tatctttcac actgttgctc tttctttaat aatactaaat aatatctttc acattgttgc
      181 tttttcttaa attacactaa ataatatctt tcacattgtt gcttttcttt aataatacta
      241 aataatattt tccacattgt tgctttttct aaacaactat aaataatata ttaaatcctt
      301 atttcttttt ctaataagtt aaataataca ttttacacat atctatttgt cttgcacata
      361 ataaataata cattatattt ctatctaaat ttagattcta aattaaatag tataaattat
      421 attcttcaat aaacaagttt tcaaatccaa gattcgctct ctatctttaa actttattgt
      481 aaacataatt ccatcttttt taattatttc tccttcatga aattttttat ggtatacctt
      541 atcccctaca ccaatactgt caatttcttt tttcgttgga gattttatat cattaataaa
      601 taatgattta tcaactttct taccatactt atttaaagta gagcttatac ataacttttc
      661 ttcagctctt gtaatagcaa catacataag tcttctttct tcttctaatt gttcttcttt
      721 tttctcttcc ttacaaatat catatgattt ttcatgaggt attgttcctt catttacacc
      781 tataatataa acatttctaa attctaatcc ttttgcacta tgcatagttg taaatatgac
      841 accctcagtc tgcttattct tgttattttc cgttaactca gtctttactc tatcaatatg
      901 ctctaaaaac tctgtaacag tattaaaatt agttgctgaa ctctcaagtt catttaatat
      961 ctctacaagt ccattagttt ttatctttct gttgctacaa taatctaaaa cgtatctatc
     1021 ataatcaaga ctagttctaa tataagatat tgcattttta ggatttaaag tatttaaata
     1081 acttaaatct atttccaaat catttattgt tttaacttgt ttaggatgaa gattacactt
     1141 atttattaat gctgttataa agtctttttc atccttaacc atatttatac tgtctttgga
     1201 tatatatcta aaaggcttgt ttataattct caaccaatct ttatttgatt ttggattaat
     1261 acctattctt aaataagcta gtatatcttg acttgcccaa tggtcatata ttgtaattac
     1321 tgagtctttt actacaaatg gaatcctcat atccataaat acatctacta atgctcttga
     1381 ctgtatattt gttctataaa ttactgcaaa atctgaatac tctacataat ctttttgtat
     1441 ttcgtcaatt atttccttgg caatatataa ggcttcttcc tctgaatcat gtggagaaat
     1501 gtaggtaaca cttcctccat ccccctgact acacttaatg actttttcat atctattttt
     1561 atttttgtcg ataagtctat ttgcagtata cactatctca cttttagacc tataatttat
     1621 atctaatatt atttttttag tattattaaa gtattgttca aattcaagca aaaaatctgg
     1681 tctagcacct ctaaatccat atatactttg gtcttcatct ccaactgcaa atatattgtt
     1741 taatgggcta caaattaact ttaaaacttc aaactgtact ttatttatat cttgaaattc
     1801 atctatcaat atatatttat acacatttct aaccatttct agtgcagact tattgttcaa
     1861 aagtaaataa taagttctta taagcatatc atcaaaatct attttgttaa ctttagattt
     1921 ttgttcttcg tataagttgt atacttttaa aaactcatct tttgttaata cttctgaatt
     1981 aaattcattt ttatccataa gttcattttt tacataggag atttcattta taacttgtcc
     2041 tatattctca tcatcatcag cattctctat atttaaactt tttaggattg ccttgattgt
     2101 cattcgcttt gacttttcat caaatatact gtctaaattg tatctttcaa agtatcttag
     2161 aattttaaaa aatacagagt gaaaagtacc ataggttacc ttattaagtc ttatatcatc
     2221 acttaaattc aaagctctat ttttcatttc tattgaagat gccttggtaa aacttattgc
     2281 caagattctt gttggtgcta tatttttgtt tacaaccata ttggctattc tatatgtaat
     2341 tactctggtc ttacctgaac caggccctgc aagtatcatg caaggcccat ctatatgttc
     2401 aactgcttct aattgatttt catttaactt tcttatatct atgtttgttg tgattattag
     2461 ctccaccacc ttaatttata attatattat tttcttttta attataaact aaaagttcat
     2521 aaaaataaat ttaatttttc ctctattaaa ccacatcatt cctattttca tgacatatct
     2581 tgagctcttt tttaattttg tcacttatat cttcaggtat aaatgatggt ttactaatat
     2641 agtaaccctg catataatct actccaagat taatcagtgt ttccatttca ccttttgttt
     2701 ctacaccttc tgatatcact ttaatatttc tttcgttaga atatgagata agatttttta
     2761 atattttctg tctgttttca tctttatcaa tacctcttac aatagacata tcaatcttta
     2821 catagtctgg cattatatat aatagtacag catctccgtt gtaacctgtc ccaaagtcat
     2881 ctaaagcaat ttctatattc caatggtcta tatatgcttt ctttttctca ataaaatctt
     2941 cattaatctt atcattctct gttatttcta atactactct atttaaatat tcaccatata
     3001 aatcttcaat ttcctcaaaa tctacttttg ataaattatt attaggaata gaatttaaga
     3061 acagtttgca atcgccaaat ttttctttgt attttttaaa tgatttaagt gctttaaacc
     3121 atgtaatacg ctcaatttga tataatttag actgtgaacg agctaatcta attacatcta
     3181 ttggtgaact aaaagttttt attttaggtc tcataagtgc ctcatatgca aatactttac
     3241 ctttacaatc tacaataggc tgaaatgcat actcaactaa ttgaccatca attaacttgt
     3301 ttaattcttc tctattactc aataagaatg aatccttatt atatatatta atatcaaatt
     3361 cactaacttc ccccttgatt gtatttttaa tttgatacat agcaaaatct gcgtatctca
     3421 ttaattcatc atagttggtt gaatcatcag ggtaccagga aattcctgct gatgccctta
     3481 ttttaaattc tgttccatca tgaagtttta ataaggtatt attcatctgt ttcttaacag
     3541 aattaataat atctcttata ctatctttac tttcatatcc atatataaat acataaaact
     3601 catcacctga aagtctagat actataccat tccatgatat aaacttctta agtatgtctg
     3661 cagcacacct aatatattca tcaccagcat catgtccata tgtatcatta atatatttta
     3721 gattatctaa atcccacata ataaatgctg ctatcttaag ttcatcttta ttcttaaatt
     3781 tttcttgtat ctgtgaatga aaagctctcc tatttaaaag attcgttaaa atatcaaaat
     3841 ctctttcata ttctatcttc cttttctcaa taaattcttt tgtgacatca acaacaacac
     3901 caagaacctt tagctcatct tcaaagatat tcattcttat ccacttaagt tttttatctt
     3961 ttgtacaaaa aacattctct gcgtttttgt ataactcaaa agccccgatt ggcatatcta
     4021 ccatttcaat aatctgtgat aattttgatg aagaatctgc aacatttgca ctgagtaact
     4081 ctatagctga agataattca tctatttcag atatatttat cttatcaaga tttactggtt
     4141 ttgatggatt actgtttcta actttgttgg ctaaaagaac aattggtttt gtaaataatc
     4201 ttgatgcaat gtaaatacct attagaccta ttaataaaga aattgctata gatattataa
     4261 ctaatttctc tatccttttt gaaaatgaca aaagactttt ttcttcaata atacccaaaa
     4321 gagcccaacg ttcattttca aatggagtat tggagttata taaatctaag tattgtacac
     4381 tagcataaat gttgccatta ccacattcat tatctttatt tttaatctta tatatatttt
     4441 tatacgattc cttctgctct aaattagaat gtaaaccatc ccaaaatttt tcttttaatg
     4501 agtatccatt tgaaattaca ttttcaaagg ccatatcatt atttctatct acacctaaaa
     4561 tataattacc ttttttattt ccatttatct ctgagtatgg aagtaattct cttaaataat
     4621 ttacacttaa atctattcca agtactccat atacagttcc atcttcataa ataaggggaa
     4681 ctgtatatgt cataagccta tcacctgcat ttgtcaataa aaacttactt ccccagtatc
     4741 ccaaattaga acttgatacg tttggattat caattgcagc tctaaatggt ttataaaaaa
     4801 aatcatctcc ctcttgctca ttttcacttt taaatgaaaa tttaggttcc caataactgt
     4861 ccatagatat tccaaaactt cttgctatat ctgatgaacc tctctcaatt aacaaatcag
     4921 aattatcatt tggattaaac ttagggtcta aatcccttat atacaatcca gtcctactta
     4981 tttcaccttt actatctatt ggcttatcgt taatattttt gtcatttaga attataaaag
     5041 ctcctgtaac agagtttttt ctcaaaagat atattaaatc atttgatatg ctttttataa
     5101 tatctttgtt aatttcttca ttgttgccta catcctcaat taaggcattt ttttcattta
     5161 atattttttt tacagaggaa ttaattgtag cttccgtttc acttgtattt gaccatcttt
     5221 gtaacatttc attttgtata taatttttac gattaattac tctttcattt aagatgtcaa
     5281 acgaattatc atttaatttc ttaatagttc ctccccataa aattgtggca caaaataaac
     5341 ttgtttgaat tagcataaca ataattaggg gaaatatcat ctttttcatt attgaattgt
     5401 tttttcttag attgttttcc atacttattc cacctatacc tttgtgcttg tctacacact
     5461 aactgccaat tcatctattt ttttatagca ttttcaagct cagccttaaa actttcaaac
     5521 caagtttcaa aattcttatc attattaaat tgtgctaccg catccttctt agaagttcca
     5581 ctttttacca attcatttat tttatttgaa tcttctttag ctttatcaag cattgaatat
     5641 tcaagtattt gtctagcttt agttccacca tcaaatgctt tattagtgta taaattacaa
     5701 ctgttaattt gttcaataga tgaggctatt gaaccttcaa ctttatcaga taaactcaaa
     5761 ttatctttct tcataacttc ttttataaca ttcaaatcat tcgttttttt ctttactggt
     5821 aaatatccag aagcaataga gaattttata ttaggctctt tatctgtaaa ccattttaaa
     5881 aattctactg atgcatattc ttcttcttta gtagattttg ttacagccat tcctgcacct
     5941 tgttgtacag catattttcc ttctccttta aaacgtggtg cttgaaaaac agatgtttca
     6001 atattataac tctcattatc atttagcatt actctatctg gaaagtatgc tgctcctgaa
     6061 ctagagccta caagagctaa tatatcacct gtttttgcat catcagaacg aaacttacca
     6121 taagaagcaa aatatccatt tatatatggt acatagaaat tatcccatag ctttttcata
     6181 actttttctt caatatttag tgtaacttta ccatctttaa cagaaaaaat ttctactcct
     6241 agttgtttac ttcctataat aatataattt gctaaagcat ctctcccaaa aaatgcttta
     6301 ccatcattag gctctttcgt taaactatct gtccattcat aatatttttt tgatgtctct
     6361 actaatcctt caattgtttt tatatcattt atatttgaat ttgtagcttt tgcaaattta
     6421 tcccaatcag ttttatttaa catcaaaatt tcagttgatt ttgcaattgg gaaaagttta
     6481 aactcatcct tagaatttat tctgccttct tctacatatt cgtctatgta ttcatttaat
     6541 tcttctttac ttatgtactt atccatatct actagtaacc ctaatttatc catttcatat
     6601 gcagtatctg agtatgctgc aaatatattt ggcacatctt cagagccaac ttttttattt
     6661 gcagaatcta aaactttctc tgataaatca actacattac cttgactaaa cgcttcaaca
     6721 ataattcctt tttcagcacc tatagtgtca ttaaattcct taacaagctt atcaaaagcc
     6781 attttttgct gtccattata ataatgccaa atttgtattg atgtcggatt ttctgggtct
     6841 aacttataat tatcttcctt ttttaaacta catcctgtaa gtaataaaac aatcattgat
     6901 gtgataatta attttgcaaa cttacaccta ttcataaaat ctactccttc aaattataaa
     6961 atttataata gcattaaaac tgtaacttca ctatttatat taattataga acgtttattg
     7021 atatatttaa acaggattta caacatattt aaaagcaatt aagaaacata ctattacaaa
     7081 tatagaaaat aaagctttta cataattttt atttctaaat gcaccaaatg acaccaatcc
     7141 ttgttgaata gctactgtaa ttactaaaag tatcattatt atattaagtt gaaccaattt
     7201 aaataagtaa agtaatgaaa acaccactag aagtacacat aatacaatac tagatttatt
     7261 aataatatca tcaatcttat ctaccttctt agactgttta ttatctttat ttaatttttc
     7321 tttatttaaa ttctcaactt ttttattata tctttctctt gttttatctt tatatttcgt
     7381 atttttattt ttattcccca tttatttccc atcctctata aaattttttt aattgagctt
     7441 atatattatt taaatattat atatttaaga taccatatat tcctacaaaa aggtaactta
     7501 ctatgtatct atttttaatt ttcgcataaa aaagccatct cctttagaat taattctgta
     7561 ttgagacagc taatttaact atttatcctt tgaaacattt tctacattat catatttact
     7621 atcaactaaa actatatcaa ctcttctatt ttttgaccta ccttcttcag atgaattttc
     7681 tgctattggt ctaaactcac catatcctac agcagataat ttatctggtg ctatacctgc
     7741 attgtcaata agaagttgaa caacattggt tgctctcata actgataaat cccaatttga
     7801 cttaaactct gaatttttta tagacatatt gtctgtatga ccttctactc tcatgtaact
     7861 attcatttca tttaacatct tacctatttg aataatctta tctctagaat tatcctttac
     7921 aactgctttt cctgtatcaa atagtattgt atctttaagg cttattacta atcctctatc
     7981 ttgaactctt aatgatactg aattagccat accattttca tttaaatact tctggatttc
     8041 ttcaccaact tttttaaact tagcattttg taaactatct aaatcattac ttcctggttc
     8101 aactactggt tctattttgc tattaccacc ttcaataact ccagaagaac caccaaaagc
     8161 agaatttaga gattgagata attgtttata tttctccgca tcaacattac tcatagaata
     8221 cattattaca aagaaaatca taagtaatgt tatgaggtct gaataagtta aaagccatcg
     8281 ctcattgttt tcttcttttt catcttcatc atacaacata aacattccat ccttttatat
     8341 tattcagctc taccaccaat ttcttcaagt tcaattagtt cttttgaatt taaaaatatt
     8401 tttaattttt ctgtcattaa attagaatta tcaccctcaa gtatggataa tattgcttct
     8461 atcaccaaaa gtctttcact tatttcctgt tgatttatag ccttaagttt tgatgctatt
     8521 ggtaaccata ttaaattggc agaaccaaca ccatacaaag tagccaaaaa tgcaacagaa
     8581 atttgaggtc ctaatgcatc tggattactt gaaagattac ctaaaatatg tactaatccc
     8641 ataactgtac ctattatccc cagtgtaggt gcatatccac ctgctgaatc aaaaattgct
     8701 attccttctt tatgtcgttt tgatgttatc tgtatctgtg attccaatat atttcttata
     8761 gtaactgcat ccaccccatc taccataagt tcaagccctt ttctaataaa agggtctaat
     8821 tcatcattct cacttatttc tttgtcaaca ctaagaagcc cctctttcct agcctttgtt
     8881 gcaatttctt tgaaatagat tatattttct atgttattag gctttttatt tttaaaagct
     8941 aataacatta tctttataga ctttttaaac acagggaatg gagtcgaagc tccaacagcg
     9001 cctatagttc caccaaacac aacaagtgct gatgaaaata caaataatac tccaatatgt
     9061 cccccatcaa taacaaaagc aactaccaaa gatgctatcc ccactatgaa aaaaattaaa
     9121 gttgtcatct attccctcct ttctttaatt atacatatct aatcatttat gacattataa
     9181 aaagactatc tttaataaaa atagccttta acaattggcg gagaaggagg gattcgaacc
     9241 ctcgcaccgg ttacccagcc taatccctta gcaggggatc ctcttgagcc acttgagtac
     9301 ttctccatat ctctatacta gtaaattata taataaaatc atattttagt caattgtttt
     9361 ataaaatttg agttattttg aattattaaa catctatttt tacttttttg cttaaatgta
     9421 agttttaaat atggtaaaat tatcttgcat aacacttata atattatttg ataaatatac
     9481 atacattaac aaatatacct aaactctgac ttaattgtaa gttatgccaa aactaaaata
     9541 atactagtcc aaggaggata ttttatgtta ccacaggaaa gatacgagaa gattttaagt
     9601 attctcaaat cagataatat aattaaaata aatgacatag ttcaaatgtt tgatatatca
     9661 atagaaactg cacgaagaga tttagcccat atggaaagta tcggtctttt aaaaagagtt
     9721 tatggtggtg ctataccagt tatagcaagt agcgtagaac ttaactatgt taaaagaaaa
     9781 aaaataaact caaacgataa aaaacagatt gctattaaat gtagtgagtt tataaataca
     9841 aatgatacta tttttatgga tactggaact actgtacttg aagtttcaaa aaatttgaaa
     9901 gataaaaaga atctaactgt aataaccaac tcaattttag ttgcaaatga acttatgaat
     9961 actaatataa aagtatatgt gcttggtggc tatcttagag aaactgaagg ttctttttct
    10021 ggtccactta cactctcagc tattgaacaa tttaatgttg ataaagctat tttaggtgct
    10081 ggaggtatca ctcttgaaaa tggaatctct gactatcact tagaagaagc actggttaga
    10141 aaaaaaatga ttgaaaggtc tagaaaatct atagtagttg tcgaacctcc taagtttggt
    10201 ataaattcat ttgcatcagt agctcctata gaacacatag atactattat tacctctgct
    10261 agtatagacc ataatataat aatagagttt tctcaaaaag gaataccttt agtcatagct
    10321 gataattagc ttgtatgatt gatttttagt tccttgatgt aataaattaa aattaaggag
    10381 ccaaataatt atttatttag ctccacttat gtctaaacta tactttatat tttaattatg
    10441 taactacttt cagttagctt atcattttta gttatttgat tgccatttta tttaatttat
    10501 cagttttggt tattcgatta ttttttaatt gtctgtcttt ttaatttatt tttagttatt
    10561 taaactccta ctttaaaata acttaaattt gacttaagta ttgtttattc tacactgcat
    10621 ctttcttcat tttaatactt tgttcttctt ccaaagttaa atcttttgtc ataagtatag
    10681 caagtgcaat tgccaatact cctccaacca attttccaat tacaacaggt gtaatcatct
    10741 ctggaactac tcctgctgta aatcctaaat ggtcccccaa agctgctgta gcacaaacca
    10801 gccaagcagt atttactact ttcccctttg gactcatatc tttcatcatt ttatatactg
    10861 gaatactatt ggctaaagta aaaactaatc ctgctgcact agtggcatcc atacctaatt
    10921 ttccacccac tattgtaagt ggtttattaa gaactttaac taataaatgt aatattggaa
    10981 aagtacctaa taacactatt gcaatacttc caattacttg aataccttct cttataggtg
    11041 tcatgccttt tataagtact atacctgtta tttcttgaaa tgctgctgct cccaaaccta
    11101 tcgttatcaa tatagatata aattttccaa aaactaatga cccatttatc ataagtgtag
    11161 aattaaattt taaacctact gccaatatta ctgccaatat tatcactggt atcatattta
    11221 taattaccat tgataagtta aatcctgcta ttaatcctcc tataatacct ccaaaaggta
    11281 ttgttatcaa gcctatcagt aacccttttg caaagaatgg tctgtcttca tattctataa
    11341 gacctagtcc tactggaatt gaaaatacta atgtacatcc aagcatggat gacacgacta
    11401 atcctgcaag cattcctgcc tctttattgt gtgcaagttc catagccaat ggataccctc
    11461 ccatatcatt cgctaaaata gttgcaaaca tagatgggtc agctccacag agattgaata
    11521 ttggtattat tataggacct agtacatctg aaattactgg agctaaacaa actataccaa
    11581 ccattcctaa agccaatgga cccattgaat taaacccttc ttcaaactgc tctccaagtc
    11641 caaatttatt tccaattatt ctatctaacg ctcctaaaag cactcccaca gccattatgt
    11701 ataatataat tttatcaaag ggcattttta attcctccta tacatgtaaa atactctatt
    11761 gtaacttaat tagcacacac ctttttggta tcgttttccc aattcatagc tctttttact
    11821 gcttgattcc atccaagtaa tatttcttct cttttttgct tagtcatatt tggaatatat
    11881 tttttgctta ttgtaaattc acttctaatt tcatcaatac tgttccaaac accacaggaa
    11941 agccctgcca tataccctac accaagtact gtcgtttcag gattttctgc aatttcaatt
    12001 ggtatatcta atatgtcaga ttgaaattgc attgtaaagt tatttttagt aattccacca
    12061 tctactctca acatttttat ttctttccct gaagccaaat tcatggaatc aacaacatcc
    12121 ttgacttgaa gtgcaacaga ctcaagagta gcccttacta tatgttcttt tttagtactt
    12181 cctgttatcc caaccattgt gcctctagca tattggtccc aataaggtgc tgctatacct
    12241 gcaaatgctg gtacaaaata tacccctcca gtacttgata tggaatttgc catgtcatca
    12301 gtttctgaat aattttttat aactccaatt ccatctctaa gccattgtat tgcagcacct
    12361 gctatataca ctccaccatc ataagcatat gtaactttat ctcctatctt ccaagcaact
    12421 gttgtcaaaa taccattacc tgctatattt aactcctcac ctatattcat atataagaaa
    12481 catcctgtac cataagtatt tttaaccatt cctggttcaa aacatccatt gccaaataaa
    12541 gctgctggtt ggtctaccat tgaagcagta atttttatag aaaccccaca aaatgcctcc
    12601 ttatctgtag ttccaaaatc accactagta ggcattatat ctgctaaaat acttctaggc
    12661 actcctataa tgtcaagcaa ctctctatcc caatccatat ctcttatatt aaaaagcatt
    12721 gttcttgctg ctgtagatac atcagttaaa aaagattttc ctcctgtcat tttccatatt
    12781 aaccaactat ccaatgttcc tgcaagtact tctccatttt taattttttc ttttacacct
    12841 tttacattat ctattaccca tttaattttt gtacctgaga aatatgggtc tataattaac
    12901 ccagtcttat ctattatctt attttctaaa cctggtatat tctttaaatt ttccacataa
    12961 tcataagttc tcctacactg ccatacgata gcattgtaaa gtggtttacc tgtattttta
    13021 tcccataaca ttactgtttc accttgatta tctataccaa ttccttttat atcactttca
    13081 ttgattccac tttcaaaagc ctgttgtaat acattattac tcacagtttt tgtattattc
    13141 caaatttcta tagggtcatg ttcaacatat ccagattgtg gatatatttg agtatgttcc
    13201 atataatcgc taaaaacaaa ctctgatttt tcgtttatca atactgctct tgttcctgtt
    13261 gttccttggt caattgctaa tacatatttc acttaaaatg tcccccttta ttttcagaat
    13321 taataaattc ttttttattt tttgaatata taatctattc aagttattta tattttatat
    13381 catcatgtgg atttatgcaa tattcattac aatatttttt gttattttta attatggtga
    13441 ttaaaaaagt atttttattg aaatattatt gcaatattca ttattttatt ctataatcta
    13501 gttaaaataa cacacaatat tttaatattt acacatagtc agtcattcga aaatttaaat
    13561 ttaacttttt aaggaggctt tacaatgaaa aaaacatatc tattcagtcc tggaccagta
    13621 atggttacag ataaagtcag aaattcactt cttcactatg atatatgcca tagaggaaac
    13681 gagtttatgg atttattcaa agatacacaa caaaaaatca ataaactata taatgcaact
    13741 tctaattact actcattaat tgtatctggc tctggaacat ctgtaaatga atgtgtacta
    13801 tcttctatct ttgacaaaga agatgcagtt ctgctagtta gtaatggtgt ttttggagaa
    13861 aggctagaag aaatattatt tacttataat gtaaaaacat ataagcctaa ttttgaatgg
    13921 gcagaatacc cagacctgga cattttagaa aaatatataa ttgaaaattc tgatataaaa
    13981 gtaattgcta tggtgttcca tgaaactagt tcaggaatga taaatccagt ccctgaacta
    14041 ggaaaacttg ctaagaagta taacaaaata ttttttgtag atgcaataag tgcctcagca
    14101 ggtgaatata ttgatgtaga agagtttaat atagatataa taacaggtgt tggtggtaaa
    14161 gctcttggag catttccagg ttctgcatac ctttgtgcta aagagagtat tttacaaaat
    14221 attaaagaaa atcaatgtaa gaatgtatat cttaatcttt ataaacacta taaagttgct
    14281 aaatcatctt ctcaaacacc aaatacacct aatgttactt taatatttgc ccttaatgaa
    14341 gctttaactg agtttttgga agatgacagt aaaatagaaa gatacaaaga atgttctgct
    14401 attttaagga atggcatgga agaattgggg ctaacatttt tacttccaga taagtatatg
    14461 tctaatacag taacatctgt atttctacct aaagaaatca atgtaaatga gtttatatta
    14521 ggcctagaaa gggaaagtgg ctatgttgtc tatccaggaa aaggcaaatt cctagatgct
    14581 aacatattcc aagttgcaaa tatgggagaa atatatccag aagattgtta taaattctta
    14641 gatatcttaa aatcaaaatt ggagataaaa aaagttatat aacagtttgt ttgtaaaatc
    14701 ctacaataca tcagtataaa ttaaatagac tgattttttt aaattttcct ataataaatt
    14761 atattttata tttttgtata agggtgaact ataatatgta aagttcaccc ctatttcaaa
    14821 tctttttcct tcagtataat ctttttcttt taatttattt tatatttttg acataattat
    14881 tcttacattt aattgttttt tatttttgac gcaatcgttt tttattca
//
LOCUS       DAMKPG010000088        14811 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMKPG010000088 DAMKPG010000000
VERSION     DAMKPG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.093"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            113..667
                     /gene="tcdR"
                     /locus_tag="PC621_002876"
     CDS             113..667
                     /gene="tcdR"
                     /locus_tag="PC621_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF4110021.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            998..8098
                     /gene="tcdB"
                     /locus_tag="PC621_002877"
                     /gene_synonym="toxB"
     CDS             998..8098
                     /gene="tcdB"
                     /locus_tag="PC621_002877"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF4110022.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     NGVYLDENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSNTKLILDTNF
                     IISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEVIVLDTNYIS
                     EKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNTISDKISFNF
                     SDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVSGLVYINDSL
                     YYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQNGVLQTGVFS
                     TEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQTLDDEVYYF
                     STDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKSGYTEIDGKY
                     FYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVTINNEVFYFS
                     DSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVIDDIKYYFDEN
                     GIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8220..8720
                     /gene="tcdE"
                     /locus_tag="PC621_002878"
     CDS             8220..8720
                     /gene="tcdE"
                     /locus_tag="PC621_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF4110023.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            8799..8960
                     /locus_tag="PC621_002879"
     CDS             8799..8960
                     /locus_tag="PC621_002879"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110024.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9049..9231
                     /locus_tag="PC621_002880"
     CDS             9049..9231
                     /locus_tag="PC621_002880"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110025.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9448..>14811
                     /gene="tcdA"
                     /locus_tag="PC621_002881"
                     /gene_synonym="toxA"
     CDS             9448..>14811
                     /gene="tcdA"
                     /locus_tag="PC621_002881"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF4110026.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKL"
BASE COUNT         6015 a         1564 c         2325 g         4907 t
ORIGIN      
        1 atataaaatt taatttattt gccgattata taattataat gactgattta attccaatgt
       61 tgtcaaaatt tttaaataaa tcatcattat aaatataaga gaggatgatt ttatgcaaaa
      121 gtctttttat gaattaattg ttttagcaag aaataactca gtagatgatt tgcaagaaat
      181 tttatttatg tttaagccat tagtaaaaaa acttagtaga gttttacatt atgaagaggg
      241 agaaacagat ttaataatat tttttattga attaataaaa aatattaaat taagtagctt
      301 ttcagaaaaa agcgatgcta ttatagtcaa atatattcat aaatcattac tgaataagac
      361 ttttgagttg tctagaagat attctaaaat gaagtttaat tttgtagaat ttgatgaaaa
      421 tatcttaaat atgaaaaata attatcaaag taagtctgtt tttgaggaag atatttgttt
      481 tttcgaatat attttgaaag aattatctgg tattcaaaga aaagttattt tttataaata
      541 tttaaaagga tattctgata gagaaatatc agtgaaatta aaaatatcta gacaagctgt
      601 taataaggct aaaaatagag catttaaaaa aataaaaaaa gactatgaaa attattttaa
      661 cttgtaatta atgaatttaa agaaatattt acaatagaaa tcaaatttta gaattaactt
      721 tattgtaaaa tcaataactt aatctaagaa tatcttaatt tttatatttt atatagaaca
      781 aagtttacat atttatttca gacaacgtct ttattcaatc gaagagcaaa ttaatcaact
      841 gagtgtcttc aatttaaaat gttaggaagt gaatgtatat gaaaacctaa gtagatatta
      901 gtatatttta taaatagaaa ggaggatata taaaagagtt ttagcattta gatgtaaaaa
      961 tattcaataa aaatattata gtaaaggaga aaattttatg agtttagtta atagaaaaca
     1021 gttagaaaaa atggcaaatg taagatttcg tgttcaggaa gatgaatatg tagcaatatt
     1081 ggatgcttta gaagaatatc ataatatgtc agagaatact gtagtcgaaa aatatttaaa
     1141 attaaaagat ataaatagtt taacagatat ttatatagat acatataaaa agtctggtag
     1201 aaataaagcc ttaaaaaaat ttaaggaata cctagttaca gaagtattag agctaaagaa
     1261 taataattta actccagttg agaaaaattt acattttgtt tggattggag gtcaaataaa
     1321 tgacactgct attaattata taaatcaatg gaaagatgta aatagtgatt ataatgttaa
     1381 tgttttttat gatagtaatg catttttgat aaacacattg aaaaaaacta tagtagaatc
     1441 agcaacaaat gatacacttg aatcatttag agaaaactta aatgacccta gatttgacta
     1501 taataaattt tacagaaaac gtatggagat aatctatgat aagcagaaaa atttcataaa
     1561 ttactataaa actcaaagag aagaaaatcc tgaccttata attgatgata ttgtaaagat
     1621 atatctttca aacgagtatt caaaggatat agatgaactt aattcctata ttgaagagtc
     1681 attaaataaa gttacagaaa atagtgggaa tgatgttaga aactttgaag aatttaaagg
     1741 tggagagtca ttcaaattat atgaacaaga gttggtagaa agatggaatt tggcagctgc
     1801 ttctgacata ttaagaatat ctgccttaaa agaagttggt ggtgtatatt tagatgttga
     1861 tatgttacca ggaatacaac cagacctatt tgagtctata gagaaaccta gttcagtaac
     1921 agtggatttt tgggaaatgg taaaattaga agctataatg aaatacaaag aatacatacc
     1981 aggatatact tcagagcatt ttgatatgtt ggatgaagaa gttcaaagta gttttgaatc
     2041 tgttctagct tctaagtcag ataagtcaga aatattctca tcacttggcg atatggaggc
     2101 atcaccacta gaagttaaaa ttgcatttaa tagtaaaggt attataaatc aaggactaat
     2161 ttctgtgaaa gactcatatt gtagcaattt aatagtaaaa caaatcgaga acagatataa
     2221 gatattgaat aatagtttaa atccagctat tagcgaggat aacgatttca atactacaac
     2281 gaatgctttt attgatagta taatggctga agctaatgca gataatggta gatttatgat
     2341 ggaactagga aagtatttaa gagttggttt cttcccagat gttaaaacta ctattaactt
     2401 aagtggccct gaagcatatg cggcagctta tcaagattta ttaatgttta aagaaggtag
     2461 tatgaatatc catttgatag aagctgactt aagaaacttt gaaatctcta aaactaatat
     2521 ttctcaatca actgaacaag aaatggctag cttatggtca tttgacgatg caagagctaa
     2581 agctcaattt gaagaataca aaaaaaatta ctttgaaggt tctcttggag aagatgacaa
     2641 tcttgacttt tctcaaaata cagtagttga caaggagtat cttttagaaa aaatatcttc
     2701 attagcaaga agttcagaga gaggatatat acactatatt gttcagttac aaggagataa
     2761 aattagttat gaagcagcat gtaacttatt tgcaaagact ccttatgata gtgtactgtt
     2821 tcagaaaaat atagaagatt cagaaattgc atattattat aatcctggag atggtgaaat
     2881 acaagaaata gacaagtata aaattccaag tataatttct gatagaccta agattaaatt
     2941 aacatttatt ggtcatggta aagatgaatt taatactgat atatttgcag gtcttgatgt
     3001 agattcatta tccacagaaa tagaaacagc aatagattta gctaaagagg atatttctcc
     3061 taagtcaata gaaataaact tactgggatg taacatgttt agctattctg taaatgtaga
     3121 ggagacttat cctgggaaat tattacttag agttaaagat aaagtatcag aattaatgcc
     3181 atctataagt caagactcta ttatagtaag tgcaaatcaa tatgaagtta gaataaatag
     3241 tgaaggaaga agagaattat tggatcattc tggtgaatgg ataaataaag aagaaagtat
     3301 tataaaggat atttcatcaa aagaatatat atcatttaat cctaaagaaa ataaaattat
     3361 agtaaaatct aaaaatttac ctgaattatc tacattatta caagaaatta gaaacaattc
     3421 taattcaagt gatattgaac tagaagaaaa agtaatgtta gcagaatgtg agataaatgt
     3481 tatttcaaat atagatacac aagtggtaga aggaaggatt gaagaagcta aaagcttaac
     3541 ttctgactct attaattata taaagaatga atttaaacta atagaatcta tttctgatgc
     3601 actatacgat ttaaaacaac agaatgaatt agaagagtct cattttatat cttttgagga
     3661 tatattggag actgatgaag gctttagtat aagatttatt gataaagaaa ctggagaatc
     3721 tatatttgta gaaactgaaa aggcaatatt ctctgaatat gctaatcata taactgaaga
     3781 gatttctaag ataaaaggta ctatatttga tactgtaaat ggtaagttag taaaaaaagt
     3841 aaatttagat gctacacatg aagtgaatac tttaaatgct gcatttttta tacaatcatt
     3901 aatagaatat aatagttcta aagaatctct tagtaattta agtgtagcaa tgaaagttca
     3961 agtttatgct caattattta gtactggttt aaatactatt acagatgcag ccaaagttgt
     4021 tgaattagta tcaactgcat tagatgaaac tatagattta cttcctacat tatctgaagg
     4081 attacctgta attgcaacta tcatagatgg tgtaagttta ggtgcagcaa tcaaagagct
     4141 aagtgaaaca agtgacccat tattaagaca agaaatagaa gctaagatag gtataatggc
     4201 agtaaattta acagcagcta caactgcaat cattacttca tctttaggaa tagctagtgg
     4261 atttagtata cttttagttc ctttagcagg aatttcagca ggtataccaa gtttagtaaa
     4321 caatgagctt atcctaagag ataaagcaac aaaagttgta gattatttta gtcatatatc
     4381 attagctgag tctgaaggag catttacttc attagatgat aaaataatga tgccacaaga
     4441 tgatttagta atatctgaga tagactttaa taacaattca ataactttag gtaaatgtga
     4501 aatctggaga atggaaggtg gctcaggtca tactgtaact gatgatatag atcacttctt
     4561 ttcagcacca tcaataacat atagagagcc acacttatct atatatgacg tattggaagt
     4621 acaaaaagaa gaacttgatt tgtcaaaaga tttaatggta ttacctaatg ctccaaatag
     4681 agtatttgct tgggaaacag gatggacacc aggtttaaga agcttagaaa atgatggcac
     4741 aaaactgtta gaccgtataa gagataacta tgaaggtgag ttttattgga gatattttgc
     4801 ttttatagct gatgctttaa taacaacatt aaaaccaaga tatgaagata ctaatataag
     4861 aataaattta gatagtaata ctagaagctt tatagtccca gtgataacta cagaatatat
     4921 aagagagaaa ttatcatatt ctttttatgg ttctggagga acttatgcat tatctctttc
     4981 tcaatacaat atgaatataa acatagaatt aaatgaaaat gatacttggg ttatagatgt
     5041 cgacaatgtc gtaagagatg tcactataga atctgataaa attaaaaaag gagatttaat
     5101 agagaatatt ttatctaaat taagtattga agacaataaa attattttag ataatcatga
     5161 aattaatttc tctggaacat taaatggagg taatggattt gtatctttaa cattctcaat
     5221 cttagaagga ataaatgcag ttatagaagt tgatttatta tctaaatcat ataaagttct
     5281 tatttctggt gaactaaaaa cattgatggc aaattcaaat tctgttcaac agaaaataga
     5341 ttatatagga ttgaacagcg aattacaaaa aaatatacct tatagtttta tggatgataa
     5401 aggaaaagaa aatggattta ttaattgttc tacaaaagaa ggtttatttg tatctgaatt
     5461 atctgatgta gttcttataa gtaaagttta tatggacaat agtaaacctc tatttggata
     5521 ttgtagtaat gatttgaaag atgttaaagt cataactaaa gatgacgtta ttatattaac
     5581 aggatattat ttaaaagatg atataaaaat ctctctttct tttactatac aagatgaaaa
     5641 tactataaaa ttaaatggag tatatttaga tgaaaatgga gtagctgaaa tattgaaatt
     5701 tatgaataaa aaaggtagta caaatacttc agattcttta atgagctttt tagaaagtat
     5761 gaatataaaa agtattttca taaattcctt acaatctaat actaagctta tattagatac
     5821 taattttata ataagtggta ctacttctat tggtcaattt gagtttattt gtgataaaga
     5881 taataatata caaccatatt tcattaagtt taatacacta gaaactaaat atactctata
     5941 tgtaggtaat agacaaaata tgatagtaga accaaattat gatttagatg attctggaga
     6001 tatatcttca actgtcatta atttttctca gaaatacctt tatggaatag acagttgtgt
     6061 taataaagtt ataatttcgc caaatatata tacagatgaa ataaacataa cacctatata
     6121 tgaagcaaat aatacttatc cagaagtgat tgtattagat acaaattata taagtgaaaa
     6181 aatcaatatt aatatcaatg atttatctat acgatatgta tggagtaatg atggaagtga
     6241 ttttattctt atgtcaactg atgaagagaa caaggtatca caagttaaaa taagatttac
     6301 taatgttttt aaaggtaata ctatatcaga taagatatct tttaatttta gtgataagca
     6361 agatgtatct ataaataaag ttatttcaac atttacacct tcatattatg tggaaggatt
     6421 acttaattat gatttaggtc tgatttcttt atacaatgag aaattttata ttaataactt
     6481 tggaatgatg gtgtctggat tagtatatat taatgattca ttatattatt tcaagccacc
     6541 aataaagaac ttgataactg gatttacaac tataggtgat gataaatact actttaatcc
     6601 agataatggt ggagctgctt cagtcggaga aacaataatt gatggcaaaa actactactt
     6661 cagccaaaat ggagtgttac aaacaggtgt atttagtaca gaagatggat ttaaatattt
     6721 tgctccagca gatacacttg atgaaaatct agagggggaa gcaattgatt ttactggcaa
     6781 actaactatt gatgaaaatg tttattattt tggagataat tatagagcag ctatagaatg
     6841 gcaaacatta gatgatgaag tgtactattt tagtacagat acaggtagag cttttaaagg
     6901 gctaaatcaa ataggtgatg ataaattcta tttcaactct gatggtatta tgcaaaaagg
     6961 atttgttaat ataaatgata agacattcta ttttgatgat tctggtgtga tgaagtcagg
     7021 atatactgaa atagatggaa aatattttta ctttgctgag aatggagaaa tgcaaatagg
     7081 agtatttaat acagcagatg gatttaaata ttttgctcat catgatgaag atttaggaaa
     7141 tgaagaaggt gaagcacttt catattctgg tatacttaat tttaacaata agatttatta
     7201 ttttgatgat tcatttacag cagtagttgg atggaaggat ttagaagatg gttcaaaata
     7261 ttactttgat gaagatacag cagaagcata tataggtatc tcaataatta atgatggtaa
     7321 atattatttt aatgattctg gaatcatgca aattggattt gtcacaataa ataatgaagt
     7381 attttatttc tctgattctg gaatagtaga atctggaatg caaaatatag atgataatta
     7441 tttctatata gacgaaaatg gtctagttca aattggtgta tttgacactt cagatggata
     7501 taaatacttt gcaccagcta atactgtaaa tgataatatc tatggacaag cagttgaata
     7561 tagtggttta gttagagttg gtgaagatgt atattatttt ggagaaacat atacaattga
     7621 gactggttgg atatatgata tggaaaatga aagtgataaa tattatttcg atccagaaac
     7681 taaaaaagca tataaaggta ttaatgtaat tgatgatata aaatactatt ttgatgagaa
     7741 tggaataatg agaacaggtc ttataacatt tgaagataat cattactatt ttaatgaaga
     7801 tggtattatg caatatggtt atctaaatat agaagataag acgttctact ttagtgaaga
     7861 tggtattatg cagattggag tatttaatac accagatgga tttaaatatt ttgcacatca
     7921 aaatacttta gatgagaatt ttgagggaga atcaataaac tatactggtt ggttagattt
     7981 agatgaaaag agatattatt ttacagatga atatattgca gcaactggtt cagttattat
     8041 tgatggtgag gagtattatt ttgatcctga tacagctcaa ttagtgatta gtgaatagat
     8101 aaaaatacgt taaatatatc ctcttatact taaatatata aaaataaaca aaatgataca
     8161 ctacataaag tgttctatct aatatgaaga tttaccaata aaaaggtgaa ctatgatgaa
     8221 tgcacagtag ttcacctttt tatatttcta atggtaacaa aatatttttt tatataaacc
     8281 taggaggcgt tatgaatatg acaatatctt ttttatcaga gcatatattt ataaagttag
     8341 taattttaac tatatcattt gatacattat taggatgttt aagtgcaata aaaagtcgta
     8401 aatttaattc tagttttgga atagatggag gaatcagaaa agtagcaatg atagcatgta
     8461 tatttttttt atcagtagtt gacattctta caaagtttaa ctttttattt atgttaccac
     8521 aagattgtat caatttttta agactaaaac atcttggaat atctgaattt ttctctattt
     8581 tatttatttt atatgaaagt gtaagcatat taaaaaatat gtgcttatgt ggattaccag
     8641 tacctaagag attaaaggaa aaaatagcag ttttactaga tgcaatgaca gatgaaatga
     8701 atgctaagga tgaaaagtaa gtaatggtag atataataaa aatattaaca aataaaaagt
     8761 gttatccaaa taagaatagc tgaaagttat cataattcat gaaactgata atgaaaacga
     8821 gggagcagat gccaagagac acacaagtat taaatacata taatttcgaa gcaagtgttc
     8881 attactatat ggatgacaag gtagtatatc aaacattggt tcacaaagat ggtgcatggt
     8941 cagttggtaa aatctattaa gctacattag ttacagatat cacaaactat aatagttaaa
     9001 catagaaata tgtgtaaatt gtgatggaaa ttattcaaaa acacaaaaat acgtgatgaa
     9061 ggacaaaatg atagagaaaa taagtatcaa accttaataa atgatttaat tgatagttta
     9121 aaagttatag gaaaaatata taaagaaata aaaacattaa aaaaatataa gatatgttta
     9181 caaattacta tcagacaatc tccttatcta atagaagagt caattaacta attgagtatc
     9241 tttaaattga aatgttagga agtgatttaa atatgaaaac ttaaattata aaaaatcaat
     9301 attaatttat ctttaaaaaa tagaaaggag tgtataagat ttattttcaa agtttaaaaa
     9361 caagaaaatc aatttaaatt tcagaaggaa taaatgtggt tatagaagtg gatttattat
     9421 caaaaataat agtactagga ggtttttatg tctttaatat ctaaagaaga gttaataaaa
     9481 ctcgcatata gcattagacc aagagaaaat gagtataaaa ctatactaac taatttagac
     9541 gaatataata agttaactac aaacaataat gaaaataaat atttacaatt aaaaaaacta
     9601 aatgaatcaa ttgatgtttt tatgaataaa tataaaaatt caagcagaaa tagagcactc
     9661 tctaatctaa aaaaagatat attaaaagaa gtaattctta ttaaaaattc caatacaagt
     9721 cctgtagaaa aaaatttaca ttttgtatgg ataggtggag aagtcagtga tattgctctt
     9781 gaatacataa aacaatgggc tgatattaat gcagaatata atattaaact gtggtatgat
     9841 agtgaagcat tcttagtcaa tacactaaaa aaggctatag ttgaatcttc taccactgaa
     9901 gcattacagc tactagagga agagattcaa aatcctcaat ttgataatat gaaattttac
     9961 aaaaaaagga tggaatttat atatgataga caaaaaaggt ttataaatta ttataaatct
    10021 caaatcaata aacctacagt acctacaata gatgatatta taaagtctca tctagtatct
    10081 gaatataata gagatgaaac tttattagaa tcatatagaa caaattcttt gagaaaaata
    10141 aatagtaatc atgggataga tatcagggct aatagtttgt ttacagaaca agagttatta
    10201 aatatttata gtcaggagtt gttaaatcgt gggaatttag ctgcagcatc tgacatagta
    10261 agattattag ccctaaaaaa ttttggcgga gtatatttag atgttgatat gcttccaggt
    10321 attcactctg atttatttaa aacaatacct agacctagct ctattggact agaccgttgg
    10381 gaaatgataa aattagaggc tattatgaag tataaaaaat atataaataa ttatacatca
    10441 gaaaactttg ataaacttga tcaacaatta aaagataatt ttaaactcat tatagaaagt
    10501 aaaagtgaaa aatctgagat attttctaaa ttagaaaatt taaatgtatc tgatcttgaa
    10561 attaaaatag ctttcgcttt aggcagtgtt ataaatcaag ccttgatatc aaaacaaggt
    10621 tcatatctta ctaacctagt aatagaacaa gtaaaaaata gatatcaatt tttaaaccaa
    10681 caccttaacc cagccataga gtctgacaat aacttcacag atactactaa gatttttcat
    10741 gattcactat ttaattcagc taccgcagaa aactctatgt ttttaacaaa aatagcacca
    10801 tacttacaag taggttttat gccagaagct cgctccacaa taagtttaag tggtccagga
    10861 gcttatgcat cagcttacta tgatttcata aatttacaag aaaatactat agaaaaaact
    10921 ttaaaagcat cagatttaat agaatttaaa ttcccagaaa ataatctatc tcaattgaca
    10981 gaacaagaaa taaatagtct atggagcttt gatcaagcaa gtgcaaaata tcaatttgag
    11041 aaatatgtaa gagattatac tggtggatct ctttctgaag acaatggggt agactttaat
    11101 aaaaatactg ccctcgacaa aaactattta ttaaataata aaattccatc aaacaatgta
    11161 gaagaagctg gaagtaaaaa ttatgttcat tatatcatac agttacaagg agatgatata
    11221 agttatgaag caacatgcaa tttattttct aaaaatccta aaaatagtat tattatacaa
    11281 cgaaatatga atgaaagtgc aaaaagttac tttttaagtg atgatggaga atctatttta
    11341 gaattaaata aatataggat acctgaaaga ttaaaaaata aggaaaaagt aaaagtaacc
    11401 tttattggac atggtaaaga tgaattcaac acaagcgaat ttgctagatt aagtgtagat
    11461 tcactttcca atgagataag ttcattttta gataccataa aattagatat atcacctaaa
    11521 aatgtagaag taaacttgct tggatgtaat atgtttagtt atgattttaa tgttgaagaa
    11581 acttatcctg gtaagttact attaagtatt atggacaaaa ttacttccac tttacctgat
    11641 gtaaataaag attctattac tataggagca aatcaatatg aagtaagaat taatagtgag
    11701 ggaagaaaag aacttctagc tcactcaggt aaatggataa ataaagagga agctattatg
    11761 agcgatttat ctagtaaaga atacattttt tttgattcca tagataataa gctaaaagca
    11821 aagtccaaga atattccagg tttagcgtca atatcagaag atataaaaac attattactt
    11881 gatgcaagtg ttagtcctga tacaaaattt attttaaata atcttaagct taatattgaa
    11941 tcttctattg gtgattacat ttattatgaa aaattagaac ctgttaaaaa tataatccac
    12001 aattctatag atgatttaat agatgagttc aatctacttg aaaatgtatc tgatgaatta
    12061 tatgaattaa aaaaattaaa taatctagat gagaagtatt taatatcttt tgaagatatc
    12121 tcaaaaaata attcaactta ttctgtaaga tttattaaca aaagtaatgg tgaatcagtt
    12181 tatgtagaga cagaaaaaga aattttttca aaatatagcg aacatattac aaaagaaata
    12241 agtactataa agaatagtat aattacagat gttaatggta atttattgga taatatacag
    12301 ttagatcata cttctcaagt taatacatta aacgcagcat tctttattca atcattaata
    12361 gattatagta gcaataaaga tgtactgaat gatttaagta cctcagttaa ggttcaactt
    12421 tatgctcaac tatttagtac aggtttaaat actatatatg actctatcca attagtaaat
    12481 ttaatatcaa atgcagtaaa tgatactata aatgtactac ctacaataac agaggggata
    12541 cctattgtat ctactatatt agacggaata aacttaggtg cagcaattaa ggaattacta
    12601 gacgaacatg acccattact aaaaaaagaa ctagaagcta aggtgggtgt tttagcaata
    12661 aatatgtcat tatctatagc tgcaacggta gcttcaattg ttggaatagg tgctgaagtt
    12721 actattttct tattacctat agctggtata tctgcgggaa taccttcatt agttaataat
    12781 gaattaatat tgcatgataa ggcaacttca gtggtaaact attttaatca tttgtctgaa
    12841 tctaaagaat atggccctct taaaacagaa gatgataaaa ttttagttcc tattgatgat
    12901 ttagtaatat cagaaataga ttttaataat aattcgataa aactaggaac atgtaatata
    12961 ttagcaatgg aggggggatc aggacacaca gtgactggta atatagatca ctttttctca
    13021 tctccatata taagctctca tattccttca ttatcagttt attctgcaat aggtataaaa
    13081 acagaaaatc tagatttttc aaaaaaaata atgatgttac caaatgctcc ttcaagagtg
    13141 ttttggtggg aaactggagc agttccaggt ttaagatcat tggaaaataa tgggactaaa
    13201 ttgcttgatt caataagaga tttataccca ggcaaatttt actggagatt ctatgccttt
    13261 ttcgattatg caataactac attaaaacca gtgtatgaag acactaatac taaaattaaa
    13321 ctagataaag atactagaaa ctttataatg ccaactataa ctactgacga aattagaaac
    13381 aaattatctt attcatttga tggagcagga ggaacttact ctttattatt atcttcatat
    13441 ccaatatcaa tgaatataaa tttatctaaa gatgatttat ggatatttaa tattgataat
    13501 gaagtaagag aaatatctat agaaaatggt actattaaaa aaggaaattt aatagaagat
    13561 gttttaagta aaattgatat aaataaaaat aaacttatta taggcaatca aacaatagat
    13621 ttttcaggtg atatagataa caaagataga tatatattct tgacttgtga gttagatgat
    13681 aaaattagtt taataataga aataaatctt gttgcaaaat cttatagttt gttattgtct
    13741 ggggataaaa attatttgat atccaattta tctaatacta ttgagaaaat caatacttta
    13801 ggcctagata gtaaaaatat agcttacaat tacactgatg aatctaataa taaatatttt
    13861 ggagctatat ctaaaacaag tcaaaaaagc ataatacatt ataaaaaaga cagtaaaaat
    13921 atattagaat tttataatgg cagtacatta gaatttaaca gtaaagactt tattgctgaa
    13981 gatataaatg tatttatgaa agatgatatt aatactataa caggaaaata ctatgttgat
    14041 aataatactg ataaaagtat agatttctct atttctttag ttagtaaaaa tcaagtaaaa
    14101 gtaaatggat tatatttaaa tgaatccgta tactcatctt accttgattt tgtgaaaaat
    14161 tcagatggac accataatac ttctaatttt atgaatttat ttttgaacaa tataagtttc
    14221 tggaaattgt ttgggtttga aaatataaat tttgtaatcg ataaatactt tacccttgtt
    14281 ggtaaaacta atcttggata tgtagaattt atttgtgaca ataataaaaa tatagatata
    14341 tattttggtg aatggaaaac atcgtcatct aaaagcacta tatttagcgg aaatggtaga
    14401 aatgttgtag tagagcctat atataatcct gatacgggtg aagatatatc tacttcacta
    14461 gatttttcct atgaacctct ctatggaata gatagatata tcaataaagt attgatagca
    14521 cctgatttat atacaagttt aataaatatt aataccaatt attattcaaa tgagtactac
    14581 cctgagatta tagttcttaa cccaaataca ttccacaaaa aagtaaatat aaatttagat
    14641 agttcttctt ttgagtataa atggtctaca gaaggaagtg actttatttt agttagatac
    14701 ttagaagaaa gtaataaaaa aatattacaa aaaataagaa tcaaaggtat cttatctaat
    14761 actcaatcat ttaataaaat gagtatagat tttaaagata ttaaaaaact a
//
LOCUS       DAMKPG010000089        14800 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMKPG010000089 DAMKPG010000000
VERSION     DAMKPG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.094"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            494..1801
                     /locus_tag="PC621_002882"
     CDS             494..1801
                     /locus_tag="PC621_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110027.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1887..2149
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2275..3078
                     /locus_tag="PC621_002883"
     CDS             2275..3078
                     /locus_tag="PC621_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110028.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     gene            3116..3727
                     /locus_tag="PC621_002884"
     CDS             3116..3727
                     /locus_tag="PC621_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF4110029.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3759..4430
                     /locus_tag="PC621_002885"
     CDS             3759..4430
                     /locus_tag="PC621_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF4110030.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4729..5091
                     /locus_tag="PC621_002886"
     CDS             4729..5091
                     /locus_tag="PC621_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF4110031.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5527..6228
                     /locus_tag="PC621_002887"
     CDS             5527..6228
                     /locus_tag="PC621_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF4110032.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6499..7317
                     /gene="larE"
                     /locus_tag="PC621_002888"
     CDS             6499..7317
                     /gene="larE"
                     /locus_tag="PC621_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF4110033.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7372..8520
                     /locus_tag="PC621_002889"
     CDS             7372..8520
                     /locus_tag="PC621_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF4110034.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8656..10044
                     /locus_tag="PC621_002890"
     CDS             8656..10044
                     /locus_tag="PC621_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110035.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10073..10771
                     /locus_tag="PC621_002891"
     CDS             10073..10771
                     /locus_tag="PC621_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110036.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10774..12207
                     /locus_tag="PC621_002892"
     CDS             10774..12207
                     /locus_tag="PC621_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110037.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12229..12966
                     /gene="truA"
                     /locus_tag="PC621_002893"
     CDS             12229..12966
                     /gene="truA"
                     /locus_tag="PC621_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF4110038.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13077..13284
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13537..14736
                     /locus_tag="PC621_002894"
     CDS             13537..14736
                     /locus_tag="PC621_002894"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF4110039.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
BASE COUNT         6014 a         1366 c         2637 g         4783 t
ORIGIN      
        1 aattttgtat actaaaaggg aatatttaca gtttaatggc agttttaaaa taataaatta
       61 aagctgtcat ttttatttaa aaatttttag aaaaagtaca atagtgttaa tgtgtgaatt
      121 agataaggtg tatataataa tttagatgat atgatataaa atatgttgta aattaaatgt
      181 tttctttgac aatatataca caaggatgat aatatctata tgaataaaag atagtgtgta
      241 atttgataat atgtaattat aacattatga tataattaca ataaagtatg taataatctg
      301 tagaaatttc aagctagtta tctaagtttt cggataacta aatctaaacg gaatacataa
      361 tgtattaaat aaatgatgat actaaaatga tactattttt atagaaattt attgaggtat
      421 ataattgatg aaaataatat attaaattat ataatatatt gagttttaca taaaagcgaa
      481 ggtgggaata aaaatgaaag aaattgctga tgctataaca tcagagtatt taagctatgg
      541 tatcaatgaa gatattagta tctatgaagg tagattttgc atatatttag ataaaaaata
      601 tagatgtaat gggaaaattt attataaaat gacacctcca atatctataa gttttaaagc
      661 agacataggt tgtgttgaag agatagacaa tgaagatgac aatttagttt tggattatga
      721 taatgctatt cttgaagttc atggatacaa aattatttct ataacaataa atactttaag
      781 tgaatttagt gtagaaggtt atatcaatga tgactgcata aaatctaaga actcctatgt
      841 tgaatatgta gattttaata taataaactt agataaaata cctggaaaac taattaaata
      901 caatgataag gtatacgcag gtagaattga atttgatata aatgattatg ttgtaactat
      961 agacaagcga tatgattata gaaaagaatt aaaatcagaa ttaaagtcta aatctggagc
     1021 aataataact catataggta ggattagaag aaaagatggt agaattttta gaacaaataa
     1081 tacaataaat cttttagata gaatatcaac agctttgagt tttatgtgtg gaagatatgt
     1141 agggttttgt ttggccaaag ggtatagaag tggtaatgaa gtttatagaa tatggaatga
     1201 aaatcaaata tctcctttta gatatgtacc aacatggtca gatacacttt caaattatca
     1261 taatatggaa aaatatataa gccttatgtg taaaaagcta gaagattttt attatggttc
     1321 tgctataaag agtgttgttg attggtacat agaatctctt ggaagtgcta ccatggaaaa
     1381 caatataata tctgtacaaa ttgcacttga aactttgtca tatgtaattc tagtggaaca
     1441 aagtaagata cttacagatg aagcatttga ctgtaatttg gcatctaaaa atataagact
     1501 cttacttgat acatgtaaga taccatatgg taaacatgaa ttaaatatat ttgataatat
     1561 aataaaaaat aaatttgatg atggagtgga tttagttatt tatttaagaa atagcatagt
     1621 tcatccaagt agaaagactc atagagctgt gttagaggtt gaagatattt ggaatataat
     1681 ttcaatagga acgagatata ttgaacttgt tttactcttt attttaggtt atagaggaga
     1741 atattcaaac agattagtag aaaggtgtta tggtgaagtg gaagttgtac cttggaacta
     1801 gatttttata tttattttaa aatatatatt gacatatatt gccttatgct ataaaatcaa
     1861 tatcataata ataattcaga ataacattaa atgctaagaa aagaaatagt aaaggtagtt
     1921 aatcttaaac agagagtttc cgattggtga gaggagcaat agattttact ttgaacacat
     1981 ctttgagcag gactctgaaa gagtagaaat actttattag gagatttccg gaatccttgc
     2041 acccgttata gtgctagagt ataataattt tattgtactt aaagaggtta gtgtcgtgag
     2101 gcattaatga acagaggtgg aaccacgaga gttaactccc gtcctctagc agaagctaga
     2161 ggcgggagtt ttttaatata attttttaag cgcgcagaga attatattgc aatagataca
     2221 ctttttaaaa aataatcaaa atattttaaa aattaaaagg cgagggagaa tattatgaaa
     2281 aatatattaa aaaaagtagg aatatttaca attatgttag gtttattagg tggagttgta
     2341 ggttgttcaa aaccagataa tgaaaaagat aaggatgctt caaaggaatc taaaaaagaa
     2401 gtagttgtag ggtttgataa tacgtttgta ccaatgggat ttttagatga aaagggagat
     2461 acagtaggat ttgacgtaga cttagctaaa gaaacattta aaagacttgg aatggaagtt
     2521 aagtttcagc ctatagattg gtcaatgaag gaaactgaat taaatgattc aaaaactgta
     2581 gatgtattat ggaatggata ttctataaca gacgaaagaa agaaaatagt atcatataca
     2641 gaaccatatt tacaaaataa acagataata gttactctaa gtgattcaaa aataaattca
     2701 aaagctgatt taaaagataa agaagttgga acacaacaag gttcaacagc acttgatgca
     2761 gtggaaaagg ataaagattt tatgaatagc ttaaaaggtg gagcaccagt tctttatgat
     2821 acttatgata aagcacttag agatttagag ataggtagaa ctagtgcagt tgttggtgat
     2881 gaagttttaa ttagatatta tatgggacaa aagggtgaag acaaatacaa ggtattaaaa
     2941 gacgattttg gattagaaga ttatgttgta gcaacttcta aagaaaatcc agaactttgt
     3001 gagaaaataa ataaaacatt aaaagaaatg aaaaaagatg gaacttttga caaaatttat
     3061 gataagtggt ttaaatagaa gataagttag ctgttaagaa gggggcacta aaggcatgtt
     3121 acaaggttta gagatagtta tagcaatgtt ttgtataaca ttgatagtat caattccatt
     3181 ggggattgga gttgcatttt taagattatc aaagaataaa ttagtaagtg gtattacaca
     3241 atgttatata ttgataatga gaggtacacc tttacttttg caaatgatag tcatatttta
     3301 cggattacct cttttaggaa ttgtatttga cagatttaca gctggagtag tagctttctt
     3361 cttaaattat gcagcatatt ttgcagaaat atttagaggt ggaatacagt caatcgatag
     3421 aggacaatat gaagcttcaa aagtactagg atttgataaa tttactatgt ataagagagt
     3481 aatctttcca caagttttta aaagaatact agcacctatt tcaaatgaag taatcacatt
     3541 agtaaaagat acatctttag tatatatatt aggattaaat gatattttaa gaatatctca
     3601 aatagcaatg aacagggaag caagtctgtt accattattt gaggcaggag cgatatatct
     3661 tatatttgta gctatactta ctaagggatt tgaattactt gaaaaaaaat attcttatta
     3721 tagataaagt tacaatatta taggaggagg ttttttatat gttgaaaatc aaaaacttaa
     3781 ataaatcatt taaaaaaaat agagtactta aagatatttc ttttgagtta gaagaaggtc
     3841 agattggagt tttacttggt aagtctggtg caggaaagac cacaatatta agatgtataa
     3901 atggtcttga agaatttgat agtggagaga ttataataga taatgaggta ataaaaaata
     3961 agagagatat ggcaaaaata cgaggaaaaa taggtatggt ttttcaaaat ttcaacttat
     4021 ttcctcatat gacagtcctt gaaaatataa ttgaatcccc agttaatgtt tttaaagttc
     4081 ctagaaaaga ggctgaagag agggctagag aacttttaag attagttgat ttagaagata
     4141 aattaaattc ttatccattt gaattgtcag gaggacagca acagagagtt gctatagcta
     4201 ggtcatgtgc attgatgcca aaagtccttt gttttgatga acctacttct gcacttgata
     4261 ttgatactat tcaaagagta gtcaatataa tgaatagact taaagacaaa ggtatgacaa
     4321 tattaataat tacacatgat gttgtcttct caaataatgt tgcagataaa attataagta
     4381 taaaagatgg tatagtagaa aatgttcaaa taaaagaaaa aattgtttag agttttataa
     4441 atataaacta gggtagaatt ttataatata aataaagggc aagtaaaatc aaatatacta
     4501 tgcccttttg ttatctcaaa atttaatctt atattttatg tttaagttac attataattt
     4561 ataataaaaa taaaaggact atttaaatat ataatgtgaa taaatgttta ataatttgaa
     4621 atatgataat tttttatgtt gaaagcttat aatgattatg atataaattg gttatagtat
     4681 acaatttatg ggtataacaa taaaaaatta gaaaggaatt tttataaaat gattaaatta
     4741 tatggatata caaaatgctc tactgttaaa aaagctaaaa attggcttaa ggaaaataat
     4801 ttagaatttg aagatataga tatggttcaa aaccctccta gtaaagagga gttaaaatca
     4861 atttataaga ctagtggata tgatataaag aaatttttta atacaagtgg tatgaaatat
     4921 agagaattgg gtcttaaaga tattgtaaaa actgaatctg atgataaact tttagaaata
     4981 ttggtaagtg atggtatgct tattaaaaga ccattacttt tggatggtaa aaatgtatta
     5041 ttgggcttta aagaagatgt atggaaaagt actttgctaa aagaagatta ggaagagata
     5101 ggtgtgttga ttaaaattat taattaatac acctataaat ttatgtatta aatctaatct
     5161 ttataaatat taattagcaa attcaaatca aaaaaattca tatcaacaaa tatattactt
     5221 cttattacaa attatatcat atgttttata ttaaaatatt ataatattac aacgaattgt
     5281 tacaaaatta cttattatta accatgaaca tttgttttgt gctatcatat ttatataagg
     5341 gaattgtcat aaaaaatatc ttgttcatac agttttatat agaaaaaaga aatgaatctt
     5401 ttctttatat aagtatattt tgtaaattgc ctctatattt atagtttaaa taatagtggt
     5461 tttaaaaatc tgacaacaaa aataaaactt tcaaaaaatt aaatctaaaa agaataggag
     5521 ataaatatga gtattgaaga tttatatgat tttgaatgtg ttcctttgaa caatgagatt
     5581 gatattgatg ttttatttga aaagatgata gaagatggaa aagaaaaaga atatacacca
     5641 attatagtca ttgatgaaaa agtagggcta ttaagaaaaa atatagatat gattactaaa
     5701 gagtatggtt ctcttgaaaa ctatagagag tattgtttaa caaaatataa tgaaattgat
     5761 gtaaatcaat tttttgattt taggaagagt gagatttcag aattattaaa ggagctaatt
     5821 gatttagaag atgattttta tgattataaa cattttagac caattataaa tatagatata
     5881 tttcatgaat ataacaaagt ttacatagct aaaattccaa caattaaacc atatgaagta
     5941 tttgcataca tacctattgg aggatttaat gattgtccta gagatgaaga acatatagct
     6001 attgcaaaat attggtatga aaagcatgga atctttccaa tcgcaattgg atgtgataca
     6061 gttcaatatt ctgtaaaaaa tttaactaaa gatggtaaaa aatttgatga cttatgtatt
     6121 gaattagtat tttattgtga ggatattatt atacaagggt atgaaacctt aaaggctctt
     6181 aaggatgtat taaaacgctc tacaatagtg cttttttggt gggattgata ttctatcatt
     6241 tagatagtat taaattgtta gtcaccaaga atccacttac tttagttatg gggagtgtgt
     6301 caaactaggt ttttatacac agtgtagcat agtaatatgt ttaaattaca aaggttaaaa
     6361 aaatacttta atatttttaa tattataatt aatagattta aaggtaatta taatttataa
     6421 aataaatact ttatatgtta taatcattaa gaatgtaaaa aaataatata tagttttata
     6481 gttcatattt ttccagatat ggaggtaaat tttgtgaagg aaaaagaaaa attagataaa
     6541 ttaaaaaaaa tgttacttga attaggcagt gttgtagttg cttattctgg tggagtagat
     6601 agtaattttt tgcttaaggt cgcaaaagat acattaggtg aaaatgttat agctgtgacc
     6661 attcatgcaa tgatgcattc aagtagggaa attgaagagg ctaaacaata tactcaaaat
     6721 tttggcgtta aacatatcat attaaatata gaaaactttg atttaaaaga atttaaagaa
     6781 aatggtgttg atagatgtta tcattgtaaa aaatatatat tctctaaaat aaaagaggta
     6841 gcaaaagaac ataatataaa gtacatagta gatgggacaa atatggatga tttaggagac
     6901 tatagacctg ggcttaaggc attgagtgaa ttaggagtga ttagtccatt aaaagatagt
     6961 agtttaaaaa aagaagaaat taggtcgctt tcaaaaatat taggattaaa tacttttaat
     7021 aaaccatcat ttgcatgtct tgcaagtagg attccttatg gagttgagat aacagatgaa
     7081 aatttaagaa tagtagaaaa gagtgaagaa tatctattca atttaggttt ttctcaattt
     7141 agagtgagaa tgcatggaga gatagctaga atagaagttg ggcaggaaga actaggtaaa
     7201 ttttttgaaa ataacaattt taataaagtt gatactaaat taaaaatatt tggctttaaa
     7261 tatgttacac tagatatgtc aggatataaa atgggaagta tgaatttaaa tgtgtagatt
     7321 attttaaaaa taaataagtt ttatagttaa tataaggaaa caggaggttt tatgaaaaat
     7381 tatacattaa tatcgccatg tttttttgga atggaaaaaa tgctggctag agagattact
     7441 aatttaggat atgaaataat aaaaacagaa gatggaagaa taacatataa aactgatgag
     7501 tttggaatag caaagtcaaa catgtggttg agatgtgctg aaagagtaca tcttaaaatt
     7561 gctgaatttg aggcaaaaag ctttgatgaa ttatttgaaa atacaaaaag aataaactgg
     7621 tctagatata taccttatgg agctcaattt cctatatcaa aagcttcctc tataaaatca
     7681 aaattataca gtacaccaga tgtacaagcc atagtaaaaa aggctatagt agaaagctta
     7741 aagaaaagtt atttagagga tggtttgctt aaagaagata aggagaaata ccctattttt
     7801 gtatttatac acaaagataa agtaaccatt tctatagata caacaggaga tgctttgcat
     7861 aagagaggat atagagaaaa ggcaaataaa gctccaataa gagagacttt agctgctggg
     7921 cttatttatt taactccctg gaaagcaggt agagttttag tagaccctat gtgtggttct
     7981 ggtactatat tgattgaagc tgctatgatt ggtataaata tggcacctgg cttaaataga
     8041 gagtttatat cagaaaagtg gagaactctt gataagaaaa tatggtggga tgtaagaaaa
     8101 gatgctttta ataagataga taatgaatct aaatttaaga tttatggata tgatatagat
     8161 gaagaatcta tagatatagc tagagaaaat gctgaaatag caggtgtaga tgaatatata
     8221 gagtttaatg taggagatgc aactcaattt aaaagtgaag atgagtttgg atttataatc
     8281 actaatcctc cttacggaga aagattagaa gataaagaca gtgttaaaca gttgtataag
     8341 gagcttggtt atgcatttag aaaattaaaa aattggtcgt attatttgat aacttcttat
     8401 gaagactttg aatatgagtt tgggcaaaaa gcagacaaaa aaagaaaatt gtataatggt
     8461 atgttaaaaa ctaacttttt ccaatatccg ggtcctaagc ctcctagaaa taataaataa
     8521 gttttacatg atttatttgt aaaatataaa attttattaa aataagtaaa acattatgat
     8581 gccttgaaac gtctaattat tgtgaggaga tttttgtgaa caaatttaaa gatttatagg
     8641 attaggagga gttaaatgaa agtgttaaag cttattactg tagtagccct gatgatgtta
     8701 tttgttactg gatgtaatat aaaaactgaa tctccagaac aattagcgaa gatacctgac
     8761 tatgatgata caaaaaaagt attgtatgat ggtattgacc agttactaaa accagattca
     8821 tctatgatat tgccttctaa tacaaaagaa gtaggaaaaa taaataaggt agatttaaac
     8881 agcgatggaa ttgatgaatt agtagtattt gaacaaaaag aagatttaag taataatgtg
     8941 agtcaggtag ggtttgtaac cttgagttac aatggagaaa aatacgtact tggagaccat
     9001 tttttagaaa atggcgaatc aatagaatat gctaattttt atgatttgga ctcagatgga
     9061 tataaggaag ttatcttatt ggttaaatcc aaagacaaaa cgaatatgca tatatataaa
     9121 gtagtagata atgaaataac aaaaatatat gacttggatg catcatggct tcaaaataaa
     9181 gaagatttta atgacatgaa agtaaaaatt ggatatatag atggagatga caagttagac
     9241 atattgatgc ttcatttgaa taataagact aatgaaatgt ttgcaagtgt tgcaaatttt
     9301 gatgggaaaa tgaaaatcaa agattctgtt aagtttgaaa atgtaaaaaa cttaagtgaa
     9361 ttatatacaa cattaggtaa tgttgcttca tctgttggaa attcttctgc agctataaaa
     9421 ggtattgtac ttgatattcc tatattgaaa gataataact atattacgca gatactttat
     9481 atgaaagatg ggaaaattaa caaagctttt agtgattatg ataaaactat tactaaatct
     9541 tattatatac ctgtagatga tattgacaaa gataagatta tagaaatacc tatagttgca
     9601 ggcagtacag gtaataataa aaatacttat agttctaaat catctgcaac tataagttgg
     9661 tatagatgga atggaaaaga aggtgctgac tcaagtttaa tatttacaag tcagatatat
     9721 tataattata aatataattt taaattattt attccaaata atttatttga taaaatactt
     9781 gttgaacagg aatttgttgg agaaaaagca gtatttaaat tttactattt tgactattca
     9841 gatcgtaaga atttatttaa tatagtagta gagagtaaaa acaaacttga agatagaaaa
     9901 aatacaggta ctcaaaactc tattgtactt caagagagcg atgaatatac tttcttacta
     9961 gttgtaaaca atgcaaatga aatggataag ctagatatga ctatagacgc attaaaagaa
    10021 tatttctcat taatatatgg ttaatattat actcaattgg aggttttatt ttatgaagga
    10081 aaagatactt atactagaag atgaaattgg tattagaagt tttgttagta taaacttaaa
    10141 aagagaaggc tatgaaatag ttgaggcagg tactggaaga gaagctatag aaaaaatgac
    10201 aacagaaaat gatattacaa tagctctttt agatgtgatg cttccagata taagtggtat
    10261 agaagtatgt aaatttataa gggagaattt tgaccaagta ggtataataa tgcttactgc
    10321 taaagctcaa gaggatgata aaatagaagg atttatttca ggagcagatg attacatcat
    10381 aaaaccattt agtataaaag agcttttagt cagggtttct gctcttctta gaagagtagc
    10441 aaaggatgat tcaagtgtta agtcgagtga aatagtttct ccaccattta tacttgatat
    10501 agacaagaga aaattattta aaaatggaaa ggaaattgag cttacaccaa ctgaattttc
    10561 aatagtgaaa tatctaatat caaatgctaa gcaatcgtta agtagagacc agatattaga
    10621 cgaagtatgg ggaactaact atttatatga ttttaaaata gtagatgtaa atataagaag
    10681 gataagaaat aaaatagaag atgacccatc aaagccaaaa tatatacaaa ctatttgggg
    10741 ttatggatac tgttttagaa aggaagaata atagtgttac attttgaagg tctgagaaaa
    10801 aaagtaataa aaaactattt tataataatt attataatgg ttactctgtt tgaaggattg
    10861 ttcatgtttt acatacaaaa ctactattat gattctgtca aacaactact tgaatctgaa
    10921 ataaaatatg ctgatgaata taatgctatt acaatggaaa ctacaagttt tgagaaaaaa
    10981 gtaaaaaata tatttgataa acaaccatta actaaaaatt cagaatttgg aatttcaata
    11041 atagataaag ataaaaatat tatattagac caatatggtt ttaaaagtaa agaaaaagca
    11101 aactatgaag atgttaacaa tgcattaaaa gatataaaaa ctaaaaattt aacaccttat
    11161 acttatagaa taccagatac aggggagcat gttatgagta tctccttacc acttaaggta
    11221 aataatataa tagaaggtgt tgttagatat acagtatcat tagatgctat tgataatgca
    11281 atattaaagc aagctacatg gcttatatta gcaggaatat ttatacttat tatagctata
    11341 ctaataagtc ttaaatttgc tgagactctt ataaaaccat tacgagagct aaagaaattt
    11401 gcaaatgaat tggctgtagg aaattataat ataaaattag aaaagatgaa gattgtagat
    11461 gatgagattg gtgatttagc acagacattt gaacacatgg cacatgagat tgacaaaagt
    11521 gaaaaattaa aagaggaatt tatatcatct gtatctcatg aactaaggac tcctcttaca
    11581 tctataaagg ggtggagtga aaccttaggt tatgaaagta taacaagaga agaactagac
    11641 ttaggtttag gcattataca agatgaaaca gaaagactta taaaactagt tgaagaatta
    11701 ttagactttt caaggctttc ttcagacagg ataaaacttc atgttgatat agttgatgtt
    11761 gaaggtttaa ttgtaggtgt tgtaaatcaa cttaaagtta aagcagctga aaaagatata
    11821 tcacttttat ttgaatttga aaatgaattt atagagaaca ttcaaggaga taaaaataga
    11881 ttaagacagg ttctcataaa tttaatacaa aattcattta aatttacgag tcaaggtggt
    11941 tacataaaag ttgtagcttc tcaagatgaa gagataacaa ccatatcagt agaggataat
    12001 ggttcaggta tagaaaagca aaatttaaat aaagtgttag ataaattttt ccaagaagat
    12061 tataataagg ctggtagtgg attaggatta gcaattagta atgaaattgt aaaacttcat
    12121 ggtggaagaa tgaaaataga aagtgaaaag aatgttggaa caaaaataac ttttaatata
    12181 aaaaataaat ttgctaaaca agcataaatt cttagaaaga ggattaagat gagaaacata
    12241 aaaatgatag tagcatacga tggttcaaga tataaaggat atcaaaaatt aggtgacaat
    12301 aatatgacta ttcaagaaaa acttgaaaat gttttaagta aaatgactaa tgaaacagtg
    12361 gagataatag ggtctggaag aactgatatg ggagctcatg caagaggaca agtggtaaat
    12421 tttagaacta attgtatgga ttctttggat aaaattcaaa aatatttata tgaatattta
    12481 ccagaagata tagttgtaaa aactgttgaa gaagttgatg aaagatttca ttcaagatac
    12541 aatgtaaaat caaagactta tatgtataaa attgacaata ataagtatca caatcctttt
    12601 ataagaaaat atgctactca tgtaagtaaa aaattagatt tagatagaat gagaaaagca
    12661 agtgagtatt tagtaggaga acatgatttt actagttttg cttcttctaa atctaagaaa
    12721 aaatcaaatg taagagaaat ttattctata aatataaaag aagatgataa tgttatagaa
    12781 atatatgtag aaggaaatgg atttttgtac aacatggtaa ggataatagt aggtgctttg
    12841 atagatgttg gtctaaaaag aagagctcca caagatatta agtatatgct tgaatcaaaa
    12901 gatagatgtc aatcatcaga tactgcacca gctaaaggac tatgtctttg gaaagtaaca
    12961 tattagtcaa ttatataaaa ctctctttaa aataaaaata aaataaatta tatagttgac
    13021 ataaatggaa aaataaacta ttatatattt tataagagag atataaaact ttatatttaa
    13081 atcctgtgag aaagagagta acttctatgt gttttataga gagctgagga tggtggaagc
    13141 tcagcaaata agtattagtg aaaagaactt tggaggatac taattgaatt tagtatgtta
    13201 gtaggtaatc tcgccttaag agaaagagtg gataagttta tcttatcaat tagagtggta
    13261 acgcggatat atttcgtctc ttggtttttc caagaggcgt tttttaatat atttaattat
    13321 tagtgtttat ctggtatgtt gaacatttac taattgatta gctaagttta tcaataagag
    13381 tggtaccgcg gatatttcgc ctcttagtat tttaaaatac taaggggttt ttttagtatt
    13441 aaaatgattt tatgtgagtc taaattctca gagtcattca atattaagaa tttctataaa
    13501 ataaaaatta taatttaaga ttgggaggaa ttaaaaatga aggaaaaagt agtattagca
    13561 tattcaggag gacttgacac atcaatcata ataccttggt taaaggaaaa ttatgaagat
    13621 atagatgtta tagctgtttg tggaaatgta ggtcaggaag ataaaatgga agatgtatat
    13681 gaaaaagctt tacaaagtgg agcatcaaag gcatatgtgg atgatataag tgaagaattt
    13741 gtaactgaaa ctatatttaa agccgtaaaa gcagaggcta aatatgaagg aaaatactta
    13801 ttaggaactt ctttagcaag acctataata gctaaaaaat tagttgaagt tgctcataaa
    13861 gaaggtgcaa aatatatatg ccatggatgt acaggaaaag gtaatgacca agttagattt
    13921 gaagctacaa ttgcagctct tgacccgact ataaaagtta ttgcaccttg gagaatttgg
    13981 gatataaaat caagagaaga tgctatagac tatgctgaaa aacataatat aaaggtaaca
    14041 gcaactaaag ctaaaatata ttcagtagat gctaaccttt ggcatgtttc aacagaaggt
    14101 ggagatatag agcacctaga aaatgaacat aaaaaagatg tttataaaca atgtgtagac
    14161 ccagaagatg catgtgatgt agctgaatat gtagaagttt actttgaaaa aggtgtacct
    14221 aaaaaagtaa atggcgaaga attatctcca gttgcactga ttcataagtt aaatgagttg
    14281 ggttgtaaac atggaattgg tgttatagat atagttgaga atagacttgt tggtatgaaa
    14341 tcaagaggta tatatgagac tccaggagga acaatccttt atgaagcaca taatatttta
    14401 gaaagtgcta ctttagacaa agatacatta catttcaaac aaatggtatc ttataaatac
    14461 ggagaactta tttacaatgg actatggtat tgtaaattaa gagaatctat agatgcattt
    14521 atggaacaaa ctcaagataa tgttacagga acagtaaaag ttaaacttta caaaggaaat
    14581 ataaaacctg ctggaatatt tactgaaaat gctctttatg atgaaggaat atcttctttt
    14641 ggaaatagtg aactttatga ccacaaagat gcagaaggat ttattaattt attcacttta
    14701 ccattaaaaa tcagagctat gaaagctgga aaataataaa ataaattatt taagagtgta
    14761 taaaaactat ttttaggttt ttatatgctc tttttttatt
//
LOCUS       DAMKPG010000090        14597 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMKPG010000090 DAMKPG010000000
VERSION     DAMKPG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.095"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            145..993
                     /locus_tag="PC621_002895"
     CDS             145..993
                     /locus_tag="PC621_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF4110040.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1072..2994
                     /locus_tag="PC621_002896"
     CDS             1072..2994
                     /locus_tag="PC621_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF4110041.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3076..3939
                     /gene="aroE"
                     /locus_tag="PC621_002897"
     CDS             3076..3939
                     /gene="aroE"
                     /locus_tag="PC621_002897"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF4110042.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4139..5356
                     /locus_tag="PC621_002898"
     CDS             4139..5356
                     /locus_tag="PC621_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4110043.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5592..7457
                     /locus_tag="PC621_002899"
     CDS             5592..7457
                     /locus_tag="PC621_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF4110044.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7635..8999)
                     /locus_tag="PC621_002900"
     CDS             complement(7635..8999)
                     /locus_tag="PC621_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4110045.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9146..10489)
                     /locus_tag="PC621_002901"
     CDS             complement(9146..10489)
                     /locus_tag="PC621_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF4110046.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10562..10663)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10993..11646
                     /locus_tag="PC621_002902"
     CDS             10993..11646
                     /locus_tag="PC621_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF4110047.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11787..13058
                     /gene="brnQ"
                     /locus_tag="PC621_002903"
     CDS             11787..13058
                     /gene="brnQ"
                     /locus_tag="PC621_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF4110048.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13260..14447
                     /locus_tag="PC621_002904"
     CDS             13260..14447
                     /locus_tag="PC621_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF4110049.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         5407 a         1770 c         2530 g         4890 t
ORIGIN      
        1 aataaattta taaaatctag ataaatagaa atcaaaaaat acttttattt ttaaggtaaa
       61 ataatactac tagtaggata aataaaaata taaaatcaaa gatactatta attataaaga
      121 aaataaaggg ggcaaattta agctatgaat aatcaaaaaa atcttaatat tccaattaca
      181 attagttctt atacattggg aacagaagtt tcattccgtg atagagttcg tattgcaaaa
      241 gaagctggat ttgatggtat tggattgcgt gctgaaaatt atattgatgc aaaaaatgca
      301 ggtgttacag atgaagaaat gattgcaatt cttaatgaat atgatatgaa agttacagaa
      361 gtagaatata taactcaatg gggaacagaa gaagatagaa ctgaagctca aagagaaaaa
      421 gagcaaaatg tttatcatat ggctcactta tttaatgtta aacatataaa ttgtggatta
      481 ttagagatga taccagaaga acaaattata actgcattgg gagaattatg tgacagagca
      541 ggtgaactta ttataggtct tgagtttatg ccatatagtg gtgtaccaga tttggctact
      601 gcatggcgtg ttgtgaaagg atgtaatcgt gaaaatgcaa tgcttatatt agatacttgg
      661 cactgggcta gagcaaagca gacagcagag gaccttgcac ctgttccagc agataagata
      721 gtatcaattc aagtgtgtga tgttttagaa actccttatg aaaaacttag ggatgaatca
      781 ctacatgacc gtttggctcc aggtgaagga tatggaaaca cagaggaatt tgtgaaaata
      841 ataaaagaac acaatatatc accaagagtc ataggggcag aagtaatatc tgattctcta
      901 atatctaaag ggttatccac tgctgctgaa actgtattta atgcaactaa aaaagttttg
      961 gataaagcat ggtctgaagt atctccaaaa taaacttata taatataaaa atatacagag
     1021 tattataata taaaactttt aaatataaaa cttgaaagga aggaaaacct tatgaagatt
     1081 agttctatgt ttacacctat aagaattggt tcaatgacag ttccaaatcg ttttgtagtt
     1141 ccccctatgg gaaacaattt tgcaaacaca gacggtactt taagtgaaac ttctaaggct
     1201 tattatcttg agcgtgcttt aggtggtttt ggattaataa ctatagaatc tagtgttgta
     1261 gacaaaaaag caaaaggagg tcctcgtaag ccttgtttat atgatgatag cacaatagat
     1321 agttttaaaa atgtaataga tgcttgtcat gatgcaggtt cgaaggtatc tatacaatta
     1381 caacatgctg gttctgaagg aaatgaaaaa gtagcaggac atccattaaa agctgcttct
     1441 gcaatacctg catcaaatgg tcgtaataca ccactagcaa taacaacaga agaaatatat
     1501 gaacttatag aatcttatgg agatgctgca ttacgtgcac aaaaagctgg tgcagatgct
     1561 gttgaagttc actgcgctca tggatatctg gttagtagtt ttatatcaca aagaacaaat
     1621 aagcgtgtag atgagtttgg tggatgtttt gaaaatagaa tgagattacc tcgtcttatc
     1681 attgaaaata tacgtaaaaa agtaggaaat tctctagcaa tattatgtcg tataaacagc
     1741 actgatgatg ttccaggagg aattgatata catgatagtt cagtaatagc agcatatcta
     1801 gaaggttgtg gaatagatgg acttcatgtt tcacgtagta tacatataca tgatgaatat
     1861 atgtgggctc ctacgacttt acatgctgga ttcagtgcag aattagttac tgaaataaaa
     1921 aaagctgtta gtataccagt aataacagtt ggacgttata cagagccaca gtttgctgag
     1981 ttaatggtca gacaaggaag atgtgattta gtagcattcg gtagacaaag cttggctgac
     2041 ccagaaatgc ctaataaagc taaaaatgga aaactagacg aaatgatacc atgtattgca
     2101 tgcttacaag gatgtgtacc aaatatgttc caaggaaaac ctatagcctg tcttgcaaat
     2161 cctatattag gacatgaagc agagcttaag ccagctgaaa ttagcaaaga agttcttgtt
     2221 gttggtggag gtgttggagg tatgcttgca gcatgggtat gtgctaaaag aggacataat
     2281 gtaactttag ttgaaaaatc tgaagttctt ggtgggcaaa tgcgtttagc tgcatatcct
     2341 ccagggaaag gagatattac taacttagtt agaagttata tttcaaaatg taatcaatat
     2401 ggtgtaaaaa tatgtactaa tacagaagct acagtagaac ttataaagga gaaatcacct
     2461 gatgtagtta ttatagctac tggagcaact ccacttgtac ttcctatacc aggaatcaat
     2521 gattcaggat taattcatgc tgtagacctt cttgatggta aaaaatcttg tgggaaaaaa
     2581 gtacttgttg ttggaggtgg aatggttgga tgtgaagtgg cagccttcct tggagaacaa
     2641 gaacatgagg ttactgttat tgagcttaga gaagaagttg gtgcagatgt aatttcagaa
     2701 caccgtaaat tccttatgaa tgattttgaa aattataata tacataccat tactggtgca
     2761 aaagtttcta aattttatga tgatggtgtc gactatttac ttacagatgg cacagagcat
     2821 gatttaaaag gttttgattc agtagtactt gctatgggtt ctcgtaatta cgacccatta
     2881 agtgaagtta taaaagaagt tgtaaaagaa acttatattg ttggtgatgc agtaaaagct
     2941 cgtagggcat tagatgcaac aaaagaagct tttgaagtag cattaaatat ataaataata
     3001 aataaatttt tctttgtaaa taactgaata ggttatttgc aaagaaaatg ataatattgg
     3061 gaggaaatta tagttatgga aatttcaggt agaacaggat tatttgcatt aataggtaca
     3121 ccagttggtc actctaaatc accagtgatg tataattata gttttaagaa attagattta
     3181 gattatagat atttagcctt tgatatcaca gtagataagg taaaagaggc acttttagca
     3241 ataaaaactt ttaatataaa aggagccaat gttactatgc catgtaaatc tgctgttaca
     3301 gaatatatgg atgaactttc tccagcagct cgtataattg gagcatgtaa tacaattgta
     3361 aatgacaatg gaaagttagt tggtcatata acagatggag taggctatgt tcgtaattta
     3421 aaagaaaatg ggatagaagt taaaggtaaa aagattacaa ttatgggtgc tggtggtgca
     3481 gcaactgcta tacaagtaca atgtgcttta gatggagcaa gagaaatatc tatctttaat
     3541 gctaaggatg atttttataa aagagcagag cagacagttg agaatataaa aaaagatgta
     3601 ccagaatgtg ttgttaatct atatgattta gaagatacaa acaaacttta tgaggagata
     3661 gaatcaagtg atatattgac aaatgcaact cttattggta tgaagcctta tgacaatgaa
     3721 acaaatataa aagatactag tgttttaaga aaagacttag tagtaacaga tgtcgtttat
     3781 aatcctaaga aaacaaaaat gattgaagat gctgaagcta atggatgtaa ggctattggt
     3841 ggattaggaa tgttattata ccaaggagca gaagctttta atctatatac tggattagaa
     3901 atgcctgtag aagaagttaa tgaattatgc tttaaataga taagagtgat atcttaaaca
     3961 atgaaaaaac atacgtattt caatattttt aataataaat tacaaattgt catagtgggt
     4021 tttgcatcat agtccagtta aatttgaagc tttaactgac tatactttaa atccattatg
     4081 aggatgagtt tattgtacac aaaaatgtta ataaattaac aagaatggga gaaaaattat
     4141 gaacaataaa aaatattatc ctactgccat agcgttgtat ttttcatatt ttttacttgg
     4201 tattggaatt tcaattttag gacaatacaa accagaattt tcatcaatgt ggggggctaa
     4261 aacactatca gatggaacat tggatgtaag tattgtttta gctgttattg ctgcactagg
     4321 attagggaga cttatatctt atccatttgc aggacctatt tcagataagt atggaagaaa
     4381 agtaagtggt cttataggaa actttctaca tgcaattttc tttgttggaa ttgtattttc
     4441 accaaatttt tatatagcat atgtatttgc aataattgga ggggcagcaa attcattctt
     4501 agatacctgt gtaactcctt cttgtatgga gatatttgct agtttaggaa caatagcaaa
     4561 tatgtttact aaatttacaa tagctttagc acaatttttg cttcctttta taattggatt
     4621 tgttgcagca aattcaatat catttaaagt tatttttata gtaacagcga ttcttattgt
     4681 agtagatgcc attttgatag ctgtgttacc atttccacca gctaataatg ttattgataa
     4741 caaaggcaaa acagttaaat cagagaagat gaaatttaca ccaacaagta tagctcttgt
     4801 ttgtattgga tttacatgta catctacatt tgtattatgg ttaaattgta atcaggaatt
     4861 gggtaaattg tatgggatgg ctgaccctac aaaaattcaa tccttttact caatgggagt
     4921 aatatgtgca gtattaataa cttctttact tattaaaaaa tatattaaac caataagaat
     4981 attagttata tatcctataa tagctttact tatgctatta attgtatatt ttgttcaaac
     5041 tccaactatt tgtatgttag ggggatttgt aataggttat tcagcagcag gaggtgttct
     5101 tcagttaaca acgtcaactg caaatgaaat gtttccaact aataaaggaa aaataacatc
     5161 tatagttatg attgcatcta gtatagcaaa ctatgttatt ttaaatatag caggtattat
     5221 tacaaaatca gggggagtta atggaccaaa atatgttgta ttgtttaatg tggcaataac
     5281 atttgttgga attttacttg cattgtttgt aaacatgaga tatgaaaaag aaaaagtgta
     5341 tgattatgat gtctaatatt agtataatat atgcatagag aagtctaaat ctaggataga
     5401 tttttattaa aagtaattaa aattaataag gatattattt acagttataa gatagggtat
     5461 ttacataatt aataataagg atattactag aatttcaatt tctagtaata tttttttgcg
     5521 tataaatgta aacttgacga aataagtagt ataatcaata taaattacat catgtaaagg
     5581 aggataattt ggtggagaat gtttttaagc gattgcagga atttaatggt tatgatggat
     5641 acaaagaatc atttgaaatg aattacctat gtatatatga aagtataccg ttaagagaac
     5701 aagtagaact tgcaaataat ctagtagatg aaattttaaa tatgtataaa agtgaatcta
     5761 atgaaattta tttattggag gatagtaatt ctaagtcttt aatttgttat ttcgaaattt
     5821 ttatgaaaaa gataaatact ttggtaaaag aaatgattat tgatgagaaa tggttatata
     5881 aattaactaa agaattaata tataagagta aaaaagttga gtatgtaaaa ctagggttag
     5941 ttttaagtga gaaatatcta aatgtagaaa acttaagaga agttgtagat acattttcta
     6001 aatctggaga atacgtattt tatctgagta atactattaa aaaacttgaa ttttacaata
     6061 catatttatt taatttatct aaaaaggcta caggttcaat aaaagtattt gctattgtta
     6121 atatggaaaa tttagattct aaaataaatt cctatctaat tgaagatggt tataaggata
     6181 caaagtacga acgattattg atgaactata ttatttcaat tgtagatttg aatgaatatc
     6241 ttgaaaaacg agatttagat aaagaaaaga taaataattt agcacgtctt atatgcaatt
     6301 acttgctaag tgtagaattt aaatatatag gtaataaatt agaattagtg aatcgatttt
     6361 tacctactgt agtaaattat gggactaact ttgaaagttt atattcaatt ttcctaatag
     6421 ctataaacgt cttaaaagat gaaaatatag agtacaataa gattgaattt gaaaaagaaa
     6481 taaatgatat cttgctcagt gaaaaatgga aaaatatata ttttgaagca ttaagagatg
     6541 ctagtggaaa aacagaagat attataaaaa tgtcagaaat ttatgatgta aacttatctt
     6601 ttgatgactt gttgccatat ctgaatagag atattagaga ttttgaggta tattggcata
     6661 tatctaaaaa gggaacgaca agttctagat taaaattatt aaattttttt gaagagacgt
     6721 ttaaaataga tgatttaatt ggaaaaatga aagatataga aaaagataaa ttgactcaag
     6781 aatattatga tgatatgtta ttttttattg tgcttaaagg aagtaaatca ctatatcctg
     6841 aaggaaagaa tatatcttta aaaggaatat ttggaaatat aaatgaagta agaaaagaat
     6901 ccataaatat cttaaaaaga tacagagaga aactatctct tgaagaatta aaaattgtta
     6961 aagaagcgta tgaaaaagaa aaaaatgtta tattgaagga tgaattaaga agagtattat
     7021 atgaaagtaa taatttaaaa aaagaatttg taaatattga aaaaataaaa gtagatgaac
     7081 atggtaagga tatctatttg acttcaatag ctgttgctgg ttcaaggttt agaaatagag
     7141 aatatcttga aaaagaattg gagaagagta agatatacta tttaactaga gagaaagata
     7201 atctatatga tgaaaaagct attaaaatag ttggtgaaac tggatatgta ataggctatg
     7261 tcccaagaaa agaaaactat attttgagca atttattaga tggaggaaaa ctgctgtatt
     7321 gtagagtaac tgaatataat ttatatgaag attgtatcta cgcaaatgtg tacttgagtt
     7381 ataaagatgt aattgaaaca gttgaaaact ctttaaagat ggttttagat aagagtagaa
     7441 taaaacttat aaactaagac gtttatatat tgcttagatg atgtaatcct aactgattgg
     7501 ttattttttc tggaattatg gtgtatttta tgagcaatgt taatgaatat aaaataaaaa
     7561 aacaattgtt ttgtatatat gatgtgtata aatatattta tatattcata tttacaaaac
     7621 aattgaaatt tatgttattt tatgatttta aatttagatg gaattttaac ttctgcttta
     7681 tcttttattt tagaagcttg ttttttaact ttatttaata attcagattc atctacaact
     7741 aatacttttc tttccttcat caaccattta ccatcacaaa tagtagaatc tacattttca
     7801 ctagtcatag aataaactat atttccaatt ggattatgca ttggtaaaca atgaattgta
     7861 tctggattta gaataatcat atcagctttt tttcctactt caagactacc aatttctttt
     7921 tctagtaaag cacattttgc tccatttata gtagccattt ctaagacttc ttcagctggt
     7981 acgacagttg ggtttaaagt tctaccttta tgtattagtg atgtaagata catatccctc
     8041 ataaggtcca ttctattatt tgaaggagct ccatcagtac caattgaaac aggtattcct
     8101 ttttctaaca tttcaggaat tgaggcaaat cctagaacta ctttcatagc tgcacctgga
     8161 ttatgagaaa cttttacatc atgtaatcta aataaatcaa tttctttatg agtaagccaa
     8221 actgtgtgag cagctaaaag atttggtcct aatacaccta gtttatcaag atattcaact
     8281 gttccaactc cgttgttttg ctttacaaat gaaatttcat cagctatttc agcaatatgc
     8341 atatgtatac ctgtattgta tttatcagct aactctttag tcctaataag caattcttca
     8401 gttgtattaa aaatagttct taatgcaaac catattttaa tcctctcatt agatgtgtta
     8461 ttaaacttat ggaataagtc taattgagtg ttgatttcct catcagtagt tttttgccat
     8521 gcttcaggaa gaccatctcc tgtatccata actgatttag ctaaacaggc tctaagtcca
     8581 gttttatcaa cagcttcaac catagcatct acatattgac caccagattc taaaaatgta
     8641 gttaccccag attttatcaa ttcaacacaa catgcagtag atgaaatcaa tgaatcttca
     8701 tagttgaagc tactttcgta tggccagatt ctctctctaa gccaagttaa aagtacaaca
     8761 tcatctgcaa gaccacgtcc taattgttgg gataagtgaa cgtgtgtatt gactaaccct
     8821 ggcattagta tttttccttt agcatcatat atctcagcat ctttatctac aatattaata
     8881 tcaaacttac caacattttt tataatatta ttttcaataa gtatacttcc agtttcatat
     8941 acctctctat ctgcattcat agtaattaga tatgcatttt ttataagtaa ttgtgacata
     9001 agtacaacct cttttctata tatttttata tctatgtatt atgataataa attttctata
     9061 caaagttaat agcaaaatca atttatacat aaattgattt tgctatttat aaatttaaaa
     9121 tattgaccta gaactattta aaatattaac ttagaactaa gtttacaact gcaagtaaac
     9181 ctataatcca aagtgtaata ctgattttat cagtttcttt agctaccaca tgcattatta
     9241 tatatgaaat aaatccaaag cataaaccaa tacttatact ataagttaaa ggcatcaata
     9301 aaactgtaac aaaagcaggt acagctattt tgctatcagc aaaatcaatc tgttttatgc
     9361 tttcaaacat gaatacacca accattacaa gtgcaggagc tgcagcgtaa gtaggaacta
     9421 taccaagcaa tggtgtaaat ataagtgtag ctaagaataa tgctgctgtt actattgaag
     9481 caagtcctgt tcttgcacct gctgcgatac cagaagcaga ttctccaaat gttgtaacag
     9541 tactagttcc taagaatgag cctattaaag tagaagaaac atctgcatat aacattcttc
     9601 ctaaatcttt tatttcacca ttctcatctt caagacccat ttgtttacaa caagtaataa
     9661 gaaatgctaa tgaatcaaac atgtctataa acatgaaaga gaatataggt ccaattaatg
     9721 agaatttcaa tgcacctaca atatctaatt taaaagcaac tggtgctata ctaggaggtg
     9781 ttgagattat tgctgttggc aactccacaa atccaaatat aacagatatt attgttgcaa
     9841 ctactatact tatcaatatt ccacctttaa cttttttata ttctagtatt gccattaaga
     9901 taagagcaaa aagagctatt aaaactgttg gtgtaaaatc tgcaagtgaa actatagtat
     9961 ctggattagc tattataaca cccatatttt ttaaaccaac aaaagcaagg aaaagaccta
    10021 tacctgctga agatgctact gccaattctc taggaattgc gtctgcaatt gtctttcgaa
    10081 gacctgttac tgcgagtgta aagaagacac atccagatag aaaaactacc ccaagacctg
    10141 tttgccatgg gattcctgca cctataacca aagagtatgt gaaaaatgca tttaatccca
    10201 taccaggagc tagagcaaat ggtgcatttc ctaaaatcgc aaatataagt gtaccaatgg
    10261 ctgtagctag tatggtaact gttataaggg cggctttatc cattccagct tcagataata
    10321 ttgctggatt tacgaaaact atgtatgcca ttgttaagaa tgtagtaact ccacctatta
    10381 cttctgtttt aaggctagag ccacgttcgc taaatttaaa cattttgtct aaaaagctta
    10441 atgatgaatt atttccatct ggttgtacat tgttagtgtt tggtaccatt tttacctcca
    10501 aaaaacttta agatataaac aaaaaaatat atattcatat agatagaata gaacatatat
    10561 tcaaagttga tattctattc atagaaaacc catttaaggt gagtttgtag aaactattag
    10621 accgtattac taatattata tgaacatttt ctatttgttt ttctgttgta ttatatcgta
    10681 atattgtatt atttacaata aatataataa aattattcgt ttttttgtcg attttataaa
    10741 aaattaaggt tatttattgg caagaattac tgttttcaat aattgaatat gtataagtat
    10801 aatgtcaatt attctaactt gttttatact attatataga agtctattat aaatgagttt
    10861 gtgatataat tataaaagta ctgaatacac ggatatttcg taaaaacttt ttaatatatc
    10921 aaatatgtat attaagttaa tatattaaaa aaaataatat caaaaataat ataatttata
    10981 gtggagggga aaatgactaa aattattaaa gtgcaaaaac ctaaatttac aggagaatta
    11041 gaaattattg aaaatcttga agatatactt gaagatatat ttaatgatga aaaaatattt
    11101 aataaaatta tagaaaatga agttataagt gatttagatt ctgtaagagt tagctttgaa
    11161 tcttgtatat ttagaaatgt atcctttgaa aattgcaatt taaaaaagat tgatttactt
    11221 gatgtggtat ttgaaaactg caatttatca aattcatttt ttgatggagg aagtatatac
    11281 agagttgaat ttaaaaatag taaattgata ggagttagat ttgatgattg tatacttaaa
    11341 aacgttttgt tcaaggatgt tttaggtaag tattctaact tttcattctc taaatttaag
    11401 atagtttcta tcttagattc aaattttgaa aattctgtat ttcaagaagt aaaaggtgac
    11461 aatctagtat ttgagtatac aaacttaaaa aaagcaaact ttaatggaac taaactttct
    11521 ggcatagatt ttaccactaa taatattgag ggaattgaga ttggtataga tgagattaga
    11581 ggagcaattt ttgatgtgtc acaagcaatt gatttaacga aattgatggg aattatcata
    11641 aaatgaaaaa aatattcata aaataaattg ggaaaacgtt aacaatgtgt gaatttttac
    11701 cagattgaca tttttctgaa aataaagtaa gattatctta atattcaaaa tattttcaaa
    11761 gggggtaatt tagtgtgagt aagcagatga gagatatttt tatagttggt tttgctttat
    11821 tttctatgtt ctttggtgca ggaaatctta tgtttcctcc atttttaggg atggaatccg
    11881 gcaaagactg gttaattcct cttattggat ttgttgttgc agatgctggt atagcaatgc
    11941 ttgttattat agcaacagca agatgcaagg gaagcatgga tgatgtactt aggagagctg
    12001 gtaaagggtt agcaagagta atgagtgtag ctgctctagc atgtttagca cttcttgtaa
    12061 taccaagaac ttgtgcaaca acatatgaaa tgggtattat gcctatattt ggagaccatg
    12121 gaccagtagc taaagctata ttttcaataa tcttctttgg gttaacttta gcatttacta
    12181 ttaggtcttc taaggttata gacataatag gtaagtattt aactccagcg ttattattag
    12241 tattatttgt tttaatagta aagggtataa tttctccagc aggacctatg agtcctgagc
    12301 atatgataga taaaaatcta tttggtgaag gtatatcaca aggttatcaa acaatggacg
    12361 ctctaggtgc agcatctatg gcaactataa tgttgatgtc tataatagct aaaggatata
    12421 catcagataa agaccaaata ggaatgacta ttaaagctgg ttttgtagcc tgtgtatttc
    12481 tagcagtagt atatggtggt cttacttatt taggtgcaac agtttcaact ttatatgata
    12541 ctagtatacc tcaagcatct ttattagttg aaataactag tatattgtta tatggtcaag
    12601 taggaaaggt aattttaggt gtaattgtgg cacttgcctg tctgactact tctacaggtt
    12661 taacagcatc aatatcaact tattttgaag gaataagtaa ggcgagttat aaacaactgg
    12721 ttataggtat atgtgtagct agtatgctta tatcaaattt aggtgttgat tctataatag
    12781 ctatatcagt tccaatccta caaactatat atcctgtttt attagcaata gttgttatgg
    12841 agttggctgg taaacatata aagaatgata atgcttttaa aggtgcagca tatgttacat
    12901 tagtgattag tctattatca gctataaatg gaatgacagg agctgttcca tttatacaaa
    12961 gcttgccact cgcaggtttg ggctttaact gggtaatacc agctatattg ggtggaatag
    13021 ttggtaactt tataaaaagc aacaaacaag tagcataata atttgtaatt aatttaattt
    13081 tataatatat taatttttag tactatgggt aggtttaatc taaacctaat ctacccatag
    13141 tactttttag attggattgg aatagcatgt atataatttt ttataatata atttttcttt
    13201 gaagtatgat aaaattagta atagtgataa gaaagtacaa atttaggagg ttttaattaa
    13261 tgcttagaaa aataacagca tttatgctat ccatatgttt aattattgga cttacaggtt
    13321 gtagtttact tgtaggcaag aaagataaag agaaaattat agatgatttt aaagttgcag
    13381 tggtaactca accattatct gaaaataaag tacaatacaa tatggtggaa gagatgacaa
    13441 aagaatatga ggaagagaat aaaattgata aagacaaaga tggtcaaact aaagttaaac
    13501 aaactataaa acatgttgta ttgccagaga attttacttc taatatagat agtgctatta
    13561 ataagatagt aaagttggct gacgataaag aagttcaggc tattgtagtt tcaactgacc
    13621 aagcagggct tttacctgct ttacaaaagg taaaggaaaa gcgtccagaa ataataacta
    13681 tttctgcccc tatgggagat gataaaaacc aattaagcca atttgttgat gtgaatctag
    13741 gggttagtgc agaggaaaga ggaaaagtat tagctgaacg ttcaaaagaa atgggagcaa
    13801 aagcttttat tcattatgcc tctacagatg atttgaaaga tgttaatata gccaaaagat
    13861 tagaaatgat taaagagact tgtaaaaata taggattgcc atttgtacaa gttaatactc
    13921 caaatataaa tactgaagaa gacaaaaaca aagttaagca gtttttaaat gaagatattg
    13981 aaaaacaagt taaaaaatat ggaaaagata taaatgtatt tggtgtaaat gagtatatgg
    14041 atgaagttat attgactaag gctttagaat taaagtatat agttgcagag caaagcaatc
    14101 catcaccaat acaaacgtat cctagtgtta tgggtctaaa aatatctgaa aaagatgctc
    14161 aaaattatga taaaataaac gacatgataa gtgaaaaggc taaggctttt ggtatgtcaa
    14221 atagacttgg tggatatcct atgccaatgg atgcttttct accatcagta gcaatttatc
    14281 ttgctactga aatggtgaaa caagatttaa cacaagaaga tgtatgtgac ccagattatt
    14341 tagaggcgtt tacagaatta agatttggaa tcggttcaga atttactcct ttaacagagg
    14401 tactttataa ttatcaatca gtaattttaa gccaattaat ttattaatat taaggaagtg
    14461 tctcaaaatg aactttttag tttatgaggc actatttttc tgttaaatca aatatatttt
    14521 tatcttttta acaattaaaa agaagcttat ccctatgttg agatagcttc ttttttaata
    14581 taaataagtt ttattat
//
LOCUS       DAMKPG010000091        14526 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMKPG010000091 DAMKPG010000000
VERSION     DAMKPG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.096"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..1018)
                     /locus_tag="PC621_002905"
     CDS             complement(<1..1018)
                     /locus_tag="PC621_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF4110050.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVV"
     gene            complement(1066..1926)
                     /locus_tag="PC621_002906"
     CDS             complement(1066..1926)
                     /locus_tag="PC621_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF4110051.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2205..2414
                     /locus_tag="PC621_002907"
     CDS             2205..2414
                     /locus_tag="PC621_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF4110052.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2414..2680
                     /locus_tag="PC621_002908"
     CDS             2414..2680
                     /locus_tag="PC621_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF4110053.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2709..3281
                     /locus_tag="PC621_002909"
     CDS             2709..3281
                     /locus_tag="PC621_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF4110054.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3515..4705)
                     /locus_tag="PC621_002910"
     CDS             complement(3515..4705)
                     /locus_tag="PC621_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF4110055.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4965..5396)
                     /gene="dut"
                     /locus_tag="PC621_002911"
     CDS             complement(4965..5396)
                     /gene="dut"
                     /locus_tag="PC621_002911"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF4110056.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5509..7737
                     /locus_tag="PC621_002912"
     CDS             5509..7737
                     /locus_tag="PC621_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF4110057.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7848..8333)
                     /locus_tag="PC621_002913"
     CDS             complement(7848..8333)
                     /locus_tag="PC621_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4110058.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            8585..9808
                     /locus_tag="PC621_002914"
     CDS             8585..9808
                     /locus_tag="PC621_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF4110059.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            9812..12946
                     /locus_tag="PC621_002915"
     CDS             9812..12946
                     /locus_tag="PC621_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF4110060.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            12980..14278
                     /locus_tag="PC621_002916"
     CDS             12980..14278
                     /locus_tag="PC621_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF4110061.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         5362 a         2123 c         2133 g         4908 t
ORIGIN      
        1 taacaacatt taagttatta catttattta tatatatttt ttcattatct aagtcttcat
       61 caaacatttt tattatattt tttatctcat acttgtactc atcttcattc attctataaa
      121 gcattaaatc ttctaataca tctttcatgt cgaggtacac atcttctaat aaataaaaat
      181 agccttcctc tatatctttc aatttatcta tagtatccat ttttttattt atatctttca
      241 taatttctaa attttgtata tttacatcta tgattgttaa taagtacatt ctcacagttt
      301 ttattatttc aacagaaaaa tctacaatat tataatttcc ttctttttta tataatgact
      361 ccttaaattt gttaaaacaa ttatctaaat tagaatttat atcgttaaat ggtggtctta
      421 ttatgtcacg tattattaaa agattttgta tatctacatt atttgtgatt gcctcatcca
      481 aaataaataa attatatgca tcatttaatg ggtcatgggc atctccacta aactctaagt
      541 taacaaattc aagtaaattt ttcaatgata tatagttcat acatctcata ccacaattta
      601 tatatttttc ttttatatct acaaataaat ctctaatatt atttacaaac cttggatggt
      661 tatttttttt agcacttctc atatctgtgt tattgaaaca tgtcaaatcc aaattaccaa
      721 aagtatatat tcctttaata tcagaaaata tgccaagcca ttttttaaat tttctcatga
      781 cctcttcata acttggtgca ctcttcaatt cttcagaaga tatatgtgtt aattcttgta
      841 tatgaggata taactcctca ttacttactg gtcttattaa agatttaaat ttatcaatat
      901 tttttgtttt ttcatcatac ataactgctc cgattgcaat tatatcagaa ttaaatactg
      961 ctctttttga tttagaattt ggcatattca tttcaaaatc tatatatact cttttcaata
     1021 taaacaccct ttccaattgt ttatagtgat tctttttaac tatttttatt caaaattttc
     1081 taagaaagaa tgtttctccc catctttttg ccaaccaagt actgaatcaa gatttacatt
     1141 ttctgctgcc ttaaatatag atttttctac atttttaaaa tttgattcta agctctttat
     1201 taagtctttt atctcatatc ttttattacc tgttttttta gcagcaacca tacatgcaca
     1261 atttaatggc catataccac tattttgaat aaatcttata atatgttctt ctcttatata
     1321 ataaagtggt cttataattt ttataccttc aaaattagta gaattaagtt taggaagcat
     1381 tgttttaaaa tttccagcac acaataagtt aagcattgtt gtctctatta catcatcata
     1441 atgatgacct aatgcaagtt tgttacaccc taattcttct gcctttgaat ataatgctcc
     1501 tctcctcatt cttgcacaca tatagcatgg ataatctttt gctatttcat ctgctatctc
     1561 aaatattctt gaatcaaata aatgtattgg tatatttaga tactcacaat tatctataag
     1621 taattgtctt atatttgcat ggtatccagg gtccatagca ataaattcaa catcaaaatt
     1681 aacttgtcca tgttttttta actcttgaaa cattttagcc ataagaatac tgtcttttcc
     1741 accagaaatc gctacagcta tcttatcccc ttcttcaacc aatttataat ctcttattga
     1801 cttcataaac ttagaccata aattttttct atatttcttt attatacttt tttctatatc
     1861 tcttactgga agtctttcct caaaaggaac tataatttca cagccttttc ctgatagttc
     1921 actcatctaa tcacctcgta tatttatgta tatttatgtt aataatattt tacaaaaaag
     1981 tctaccttca tccacaaaat actataattt ttactttaat tttactaaat aatttatagt
     2041 ttacaataac acataaactt ataataacat aaataacctc tttcttttat tatctttttt
     2101 atttttctca tttaatattt tattattagt aacgattttt aaatgattga atattataca
     2161 ctagatagag ttacctatct atttacaaca aggaggtttt catcttggaa aataagacta
     2221 gagatatcaa aaaaaattgt ggtccatctt tggctagacc atacattaat aatcaaattt
     2281 tcacagaaat gtactgcctt tctgatgcat taaaatttgg aactatattt ccagaactaa
     2341 acttactaga gtctgaaatg tacaacaaag aattatatgc taagccaaaa aaatatagag
     2401 gaggtagaag ataatgaaag actcatctag agctgaactt atgagaaaag ttcaggaaac
     2461 atcttttgca tgtgttgata tgaacttata tttagacaat caccctgatg ataaaaatgc
     2521 tatcaacact tataattcat tatgtaatca atttgcgcaa gctagatatg cttacgaaaa
     2581 taaatacggt cctttaacta actttgggta tgctccaagt agatgccctt ggcaatgggc
     2641 tgaccaacct tggccttggg acagagaatt taattattag acatcaagta ttggaggatt
     2701 gaaaattcat gtggatatat cagaaaacta tacaacaccc agttaatata aaaacttgtg
     2761 accctagaat ggctaaattt cttataactc aatttggtgg gccaaatgga gaacttgctg
     2821 catctttaag atatttaagc caaagatata caatgcctac tggtaatatg cgtgcacttt
     2881 taacagatat tggtacagaa gagctagctc acgttgagct tatatgtact atggtttatc
     2941 aattaacttc tgatgcaagc ccagaagagt taaaagctgc aggtcttggt tcaaactatg
     3001 ctcaaaatgg atatggaatt tatccaacag attcaaatgg tgttccattt gatgtaagac
     3061 ctatagcagt tatgtcaaat cccgtaaccg acttacatga ggatatggca gctgaacaaa
     3121 aagcacttgc aacttattat caacttataa acctaacaga tgacgttgat gttatagatg
     3181 tattaaaatt cttaggtcaa agagaaataa ttcactatca aagatttggt gaagctttaa
     3241 tggatgctta cgagttagaa gaatctcaaa aaatgttcta aaatttttat ttgtataact
     3301 attattattt gataatttat aatttactgt tgtgttaaaa tttataaaaa atataaatat
     3361 aatttatttt ctataaacag agaaaataaa taaaaagcag gtatcaatca aatagtaaca
     3421 tgtattatac tatacattac tatcaatatt gtacctgctt tcatattata agcaataaag
     3481 aaaaatctat ttattaggtt ttataataaa acaattataa aactcttctt gcatttacat
     3541 atctacttga atagtagcta ctatttatat tagatattgt tacttttttt gatgaacctg
     3601 aagatgcatg tatcatatct ccatttccta tgtatattcc aacatgactt acagaaccat
     3661 tatttgaacc ttgtgtatca aagaatacta aatctccact tctaaggtct cctctattta
     3721 catatgttcc atacttactt tggtctctag atactcttgg tatactaact cctgcagatt
     3781 ttttcataac gtactgtgtg aatccagaac agtcaaaact atttggacct tctgctcccc
     3841 atacatatgg ttttccaagt agtgtctttg caaaacttat tacactatca gctccttgac
     3901 ttggagaatt agtcgaataa gatggaattg aagtatcaac agaacctttt tcagcaaggt
     3961 attgacttga cacatatcca gtttgattgc cataacttac ttttgtccat cctccacttt
     4021 cagaaatact tccaacctca gtaccataac ttaatgtagc tattttagaa tgacttgtac
     4081 ctggtcctgt tctaacgttc aatgactttg ctgttacaac ctttgtaccc tttacaaggt
     4141 catttgaact gttttcaaca ttttcatttg tagagtcatt tacactagtt cctaaatatt
     4201 tacttgaaac ataacctact ctaccattag aacttatttt tgaccatcca tttgactcag
     4261 atatgtatcc gacttcagtc ccatatccta gtgttgatat tttagaacta cttgtacttg
     4321 gtcctgttct aaaatttaat ccttttgctg ttacaacttt tgtcgactta actgaagtat
     4381 cactactttc attgctactt ccaaggctat ttattgctac ataatttcca tgcacataac
     4441 ctgtgtttcc attatattta acttttaccc aagaaccttc ttttcctacg tactcaactt
     4501 tatcaccttt gtaaagtttc cccattgatt catggtttgt acctggtcct tttctaaaat
     4561 ttaccgcatt ccctgttatt gttctgcttt ctatactcac actctctact tcagactcat
     4621 tagcatatgc taaattcccc atagtaggca ttattatcgc agttgccatt atactggctg
     4681 ctattctctt ttgattaaaa ttcataactt tccactcatc tcctcttttc tactatcttt
     4741 atttcacttc aaaatataaa agaaataatt gacactatta aaatatcaat tatttctttt
     4801 atattaatat tattacaaat ttgtaactat ttgtctatag taatatttgc ttaccataaa
     4861 ttaccatgta taaattttaa tcttcaatag cctgtaattt agtaatatag ccatctttta
     4921 ccttttttaa aaatggctat attttttaac ttttttgtaa ctttttaaaa tccagttgag
     4981 ccaaacccac cttttcctct ttcggagtca cttaattctt ttacttcttc tatgttaata
     5041 tcatagatag gttttactat catttgagca attttcatat gcttttcaac cttaaaatca
     5101 tttttcccat gatttattaa tataatttta agctctcctc tgtatccttc gtcaatagtt
     5161 cctggtgtat ttaatacagt aacagaatgc tttaaggcaa gcccacttct aggtcttacc
     5221 tgcgcttctg tcatagtagg aagctctata catatcccag ttttgataag tttagtctct
     5281 ccaggaggta ttacaacctc ttctatagaa tacaaatcca taccagcatc acctttgtga
     5341 gcaaaattag gtattatagc atcatcattt aattttttta cttttaaatt gtacatctta
     5401 tatctccttt caactttcta taaatattat aacttaaatt tcgacctctt gaagtttttt
     5461 atttttttgt ggtaatataa ttattagtgt tttaaaggag gattttttat gaaacttgta
     5521 cttgctgaga aaccatctgt ggcaaaaact atagcatcat ttttaggtgc aaagaccaga
     5581 caagatggtt actttgaagg aaatgattat ataattactt atgcagttgg acacttagta
     5641 tcattatatg atatgaagga ttatgataaa gataaatact ctggctcttg gaaaatgaac
     5701 aatttcccat ttgttcctga ggataagttt aaatttaaga ttgatagttc taaaactaaa
     5761 caattcaaca ctgtaaaaaa gcttttgaat agaaaagatg tagaatatgt aataaatgcc
     5821 actgacaatg accgagaagg agaacttata gcatttttaa tatttttact ggcaaaaaat
     5881 aaaaaaccag taaaaagaat cttggtaaat gaatggacac cagaagatat tacaagaggt
     5941 ataaaaaacc taaaagatga atatgaaatg agaaatcttc aagctgctgg atatactagg
     6001 cttattactg actggttgat tggaataaat ttcacttctg ttgctacatt gaaatatgga
     6061 aatgggaaac tattaaacat aggtagggta attcttccaa cagttaaatt agtctatgat
     6121 agagatatgg aaatactaaa ctttgttcct aaaacttatt atgaaataga aggacatttt
     6181 aaagctgaag ctggtgaata taaaggtaaa tatgttaagg gtaaagaaag taaatttgat
     6241 acacttgaag atgcaaataa aattatagct tcaattacat ctgaaactgg taaaatacta
     6301 gataaaaaag ttactatgtc aaaggaatat gcaccaaaac tttttagttt gacatcactt
     6361 caaggatata taacttctaa gtattccaat tttacatctg ataaggtttt aaatgtctgt
     6421 caatctctct atgagggtaa tggtaagggt ggatacataa cttaccctag aactgattcc
     6481 attttcttag aagaaagttt ggcttcaaaa gcttctcaaa ctttagataa attaaaggtt
     6541 ggtcttgaat atgaaaataa aattaaattt tctaaaacta aaagagtttt tgattcttct
     6601 aaagtagact cccatagtgc tataattcca acttatataa ttccaaaaag tttatctaag
     6661 gatgaacaat tagtttatga tgccataaaa gatagatttg tagcaaactt tatgcctcct
     6721 gctgaatatg agaatacaga aattaagact gaagtagata attgtacttt cttaacaaaa
     6781 ggtaaagtgc ttaaatcaaa aggatacctt gaggtatata ataaggaaga aaaaaacgat
     6841 ttacttccac ttgtaaataa agatgatgtt gtagatgtgt tagaaataaa accattaact
     6901 aagcaaacta ctccacctaa accatatact gaggacaccc ttttaaaggc tatgaaaaat
     6961 tgtggtaaaa atgtgcctga agaagataca acagtgctat ctggatattc gattggaaca
     7021 tcagccacac gtgctgatgt gttaaagaag ataagtcaag tgggctatgt aaagaaaaaa
     7081 gggaaatctt attctataac agaccttggg aaaaatttag ttgaaatttt tcctgttaaa
     7141 gacttatttg atgtagatta tactggaaaa cttgaaaaaa gtttaagcga tattcaaaaa
     7201 ggaaagtata caagaaaaga atatcttact aatatcatta gttttatata tcaaaatgtc
     7261 aatattataa aacaagatgc ttcaaaaaat ataaacacag aagcatatac tttcgattca
     7321 aaaactaaaa aatttactaa aacaaaagaa aaaaaacaga aaaaagatgt aactaatact
     7381 gaaaaagaag ttgctatatc aaaagattct aataacaaaa aagaagttaa aaatggtgat
     7441 gaaaatcaat ctcttggcaa atgccccatt tgtcaaggtg atgtactaga atttgacaaa
     7501 ggctttgctt gtaaaaatca taaagaatgt aaatttgtta tttggaaaaa tgataagtat
     7561 ttaagtcttt ataaaaagaa agttaacaaa acaatggtca aaaatatttt aaaaaaaggt
     7621 gaaactaaag taaaatcttt aactgctaaa aacggtaata aatttgatgc tacattaaaa
     7681 tatgataaaa atccagatac cggctatttc aattggaaaa tagagtttga caactaaata
     7741 ttcataagaa aagaaatagc agtagtataa taatctatat tatattactg ctatttcttt
     7801 tcttatgaag ttttaacttt tttaatagtg acttttacaa tcacactcta acctaatttc
     7861 tttactattt ctgataactt taatgtatgt tcatctaata aagttagttc ctcttcagaa
     7921 agattagata atttttttac tagagattct ttgagaatag cttttactgc atcaaataat
     7981 ttaaatgctt tatctgttaa ttcaacccta agaatccttc tatcttttgg gtcaggaaat
     8041 ttattgacta aaccatgttc cactaatctg tcaataatcg gagtcatatt tgattttaaa
     8101 attcctaaat tagaagctat ttgagaaatt gatgatgaat ttgatgtcat aagataaaga
     8161 attaccttag catgtgatgg agccattgga cattcttcaa aataagcttt cattatatca
     8221 gagttatttt gaaacttttt taacaaatcg ctttctttaa atatattatt ttcaagcaca
     8281 aataaaaaat ctaaaaaaga gtccgctatt ttaagtatat tgttgttatc catatttgta
     8341 acatcccttc aatagataat ataataaaac attgttgttt aatatagtat aaattataaa
     8401 ctattaataa atttaaaaca agaaaaagtt attgactcat aataattata aatttataat
     8461 caaattagag aattataaat tgccaataaa gggaagtttt ttaattagtc taaaactaaa
     8521 cttaaattat aagacacttt aaattctaca acaagctagt gtgcaatata taataggagg
     8581 agatatgaaa agatatttaa tagttttctt aatgtgtagt tcactgtttt tagttggatg
     8641 tggaaaaaca aaagaatctg aacctgagaa accaatagca gtttctgttc aaaaagcagt
     8701 aggtggagaa attgaaaaca caaattcttt ctcaggaact acaaaagtta aagatgaaac
     8761 agctgttact gcacagactg ttggcacagt tcaagaagtg tatgtaaaac ttgggcaaaa
     8821 tgtaagaaaa ggagatgaat tattgagtat aagcagtcca gagctagaaa atagtgtaaa
     8881 gcaaagtaaa gcctcactag atttagcaaa agcaagttat tcaagtgcca ctggtggttc
     8941 attagaggca caagtaaatc aggcaaaaac agctcttgac aatgcaaaga ttcaatatga
     9001 ggagtctcaa agaaattatg acaataataa gattctttat gaacaggaag tcattagttt
     9061 agaccaattt aaaaaaatag agttttcttt agagcaaaca aaacaacagt tagattctgc
     9121 tcaaagagca tacgatacag caacttcaaa aagtatacct caagctaaag cacttgctaa
     9181 aaaacagctt gaccaagctc aagtatcata taatttagca atgagtaatt tagacaaatt
     9241 aacattaaca tctccagtag atggtacaat aacagctaaa aactttgatt caaaggaaat
     9301 gataacacaa tcacaaccag cttttattat ttctaatcct aatatactag aagttgattt
     9361 aaatgtagca gaatcagata taggtaaatt taagaaagat ggaaatgtag atgttataat
     9421 tgaagaccaa aggattttag gaaaaataga ttatgtacct tcagtagttg accctcagac
     9481 atctttatat cctgttaaag tacttgtaaa taatgcaaac aacaaattta aggcaggtat
     9541 gtctgcacaa gtaaacttaa gtattgaaaa agaaaatggt gctgttacag tgcctaaaaa
     9601 agcaatattt gaagaaaatg gtaaaaaata tgtatacata gcaacaaaag acaatatagc
     9661 aaagaaacac ttggttcaaa ctggaattgt aacagaggat aaaatagaaa ttaaaagtgg
     9721 tgtaagtgat aaagatacag tgattattgg aggaataagt cttatttctg acggaactaa
     9781 gatatttcct gtagaaaagg agaagtagac tatgaattta acacagactt ccgttaaacg
     9841 tcctctcaca atcataatgg tctttttagt attaatagtt tttggtggta ttggatataa
     9901 aaaaatgtca ataaatctaa tgcctgatat agaaatacca gttgtaatgg tcatgactac
     9961 ttggacaggt gctggtcctc aagacgttga tgaacaagtt agtcaaaaag ttgatgaaag
    10021 tttatctgcc gtttcaaatg taaaatctac tatatcaagt tcacaagaaa gtgtttctat
    10081 ggtagttgct cagtttgaat ttggaactaa tttggatgaa ataatgaatg atgttaggtc
    10141 taaagttgat gcattacaga ccagcctccc agatgatgca gcaaaaccaa cagttttaaa
    10201 gctggatatg aatgcacagg ctataggtca acttgtcata tctggaggaa atgaaaatag
    10261 ttcacaagcc ttaagaaaat acgctgaaga tgtaattcag cctaaaatag aatccataga
    10321 tggtgttact tcagcagacc ttaaaggtgg agaaaaagca caggttaatg taatagctga
    10381 cccagctgtt ctttcaaatt atggagttag tttatccact ataaaaggtg tcttatcctc
    10441 tagtaataaa acattccctt atggttcaat tactcaaggt gaggataaaa tagttttaag
    10501 agcaattgat aaattagaat ccctagatga tataaaacag attcaaattc ctgtaaaagg
    10561 aggaaataca gttagattag ataatctttg tagtgtagat tatggatatg cagacaaaga
    10621 tagtatttat agatacaatg gaaaagacag tttggtaata gatatttcta aacaacaaga
    10681 tgccaacact gtaaaagtta tgcagagtgc atacaaatac gtagatgaat tgaataagga
    10741 aaatcctcaa tttaaaatta aaattgctaa tgatacaagt tcatacataa gtgaatcaat
    10801 aaacagtgta atgagcaact tgcttataag tgctgttatc gcatttattg taatatttgc
    10861 tttcctaaaa agtgtaagag cttcactagt tgttgcagtt gcaataccaa tttctataat
    10921 aggtgctata gcaattctct actttactgg tgaaagttta aatcttatta ctgcaagttc
    10981 attggtaatt tcagttggta tggttgttga taatgctaca gttgtaattg aaaatatatt
    11041 taaatatagg aaaagtggca gattgagtct tgatgattgt gctattgagg gaacaagaac
    11101 tgtaacaaat gcaatacttg catcaacatt gaccacgata gcaatattct tacctatcat
    11161 gtttacagaa ggtatagcta aaataacttt tggggcatta ggtaaaacac taatagcttc
    11221 actagcattc tcctttatag gtgcaattac actcgttcct agtgtatttg caaaattaag
    11281 cagaggaaaa aatagtcaaa agatgcaaga aaaagatagt cctatatttg ataaggtaag
    11341 tgaagcatat aaaaaattat taagtgtatg tttaagacat caacgcttag ttgtaatatc
    11401 aagtttactt atttttgtag cttccttatt tggaatggga tttataggta tggactttat
    11461 gcctgcagca gacaagggtg aattatctat ttctatagaa cttccaaaag gtcttagcct
    11521 agaatccaat gattactatg tatctatgac tgagaaaaaa gtctcagata ttccagaaat
    11581 taaaacttta ataacaactt tgacatcaga ttcaaataat agtaaagcaa gtatagcaat
    11641 tgaattagaa tctcaaaaaa atagaaaaag aactactgat gaaatagaaa aagagataat
    11701 tgatagagtg gctactgtac cagattgtaa aataaatgta agtcaaaaca gtagtgttat
    11761 gggtggagct ggaggagcag acttcacatt agaaatgaaa ggtcctgatt tagatgtatt
    11821 aaaaatactg ggaaaagaag ctgaaaaaga atttagctct ataaaaggat ttagaaatgt
    11881 tgaaacttct cttacagata caacacaaga agctcaattt actatagata aacataaggc
    11941 tcaagaatat ggtgtaaata ctgctgaaat tgcaggcatg ttacgtacag caataaatgg
    12001 tgactccgta accactgcta ctattgatga ttacaaagtg gatgtaaacc taaaatttaa
    12061 agcagatact attgatagct tagaagatat aaagcaacta aaagtaaact ctgcaactgg
    12121 aaaagaagtt cctcttggtg ctttttcaga tataaaaatg gcagatggtc tacaatctat
    12181 atctaggact gatggagatt tttctgtaaa tataactgct acacttgaca atttagatgc
    12241 tggtactgct acaaaactag cactagaaaa aacgaataaa atggtaattc ctaaagatta
    12301 ctctataggt gttggtggtg aaacagaaat gatgaatgaa tctatggcaa gtcttggatt
    12361 ttcaatggtc atagctatag cattagttta tatggtaatg gtagctcagt ttgaatcatt
    12421 tagtaaacca tttataatca tgttctgtgt tccatttgca ttcgttggag ttgtgctttc
    12481 gcttctagtt agtggtcaaa gtttaagtac tgtcggtatg ctaggtataa tcttacttat
    12541 cggtattgtt gttaataatg gtatagttct tattgactac atagagcaac ttagaaaaac
    12601 aaatcgtaac aatgatttaa ttggtttggt ttcaattggt tgtgcagcaa gattaagacc
    12661 agtcttaatg actacagcaa caacaatttt aggtatgttg ccaacagcac tatctcttgg
    12721 tgaaggtgga gaaactatgc aaccactagc catagttata atgggtggtc taactgtatc
    12781 tacacttata actttggtgc ttatacctac tatatactta atatttgata aatgggaaaa
    12841 taagttttca gacaaaatgc ctaaaatatt tggtaagata acagataaaa taggaagtat
    12901 aaaacgtaaa ataataaaga aaccaaaatt gaaagctaaa gtttaaatag aatggagggt
    12961 gattgtattg aataaaaaaa tgagcaaaat agttgcaatt ggctgtggtg ttggtataat
    13021 aactgccaat ttaatcccag tttacgcaga taataatact tcttctataa acaacgttga
    13081 taattctatt ttaagtgact caaataaaag aacatctgaa aaggaaattt tgactgctag
    13141 agaagctgtt agtgctgcac ttaaaaatag tgaaaaatta aaaatgaaat cagaagaaat
    13201 aaaaatgtta aaagaaaagt tagaagtaca agatgagttt gatagtttta ctggaagtga
    13261 caactcgttt ccatacgacc aaattggact tcttaaaaat caaagtgaac aggctaaagg
    13321 ctttatggag gaccaaatag caaatgatat tactaataaa tttaacgatt tagtttcaag
    13381 agaaaatgag ctagataaga taaaaaacaa tcttgaaata aaaactaaag aaattaaaga
    13441 tatgaaatta aaaaaagatt tagggttagt aacatcttta gaaactgaat cagctgaact
    13501 tgaacttcaa acactacaaa atactcaaaa agctaaactt caagaactaa aaaacaatca
    13561 agactatttt aagttattga ctaatataga tttaaataat tatcaattgg ataaagaata
    13621 tagatttgaa tcttttagag taggtggctc agttgattca tatatggaag gtaaggttaa
    13681 tgaatattta aaatacgacc aattgatttt agaacgtaca gaagaaagct ttaatgataa
    13741 agatgagaac aaagcagatt taccagatag acctaatctt acaagaccag ttgctcctac
    13801 aaaaccagag caaggtaacc taactgatga tgaatacaag gttcttatgg ataagtatga
    13861 aaaagactac aagagatatg aaaaagaagt tgaagcatac aatcttgaaa gacagactta
    13921 tgctgctggc cttactactt atgcaaatta tcttcaacaa aaatttaata ccgaaaatgg
    13981 tctagtaaca ttagaagaca gtaaaaaagc tcttaaaaaa gggttaattg atagctatgc
    14041 tcaattactt gctttagaag atacaattca gattactaaa aaacaacttg atttatcaga
    14101 aaaacaatta aaaaatacta aattaagata tgatttagga ttaattactc taactgatta
    14161 taaaaaacaa gtggtaagta atcaagatgc taaaaatagc tatgacactc ttattgtgaa
    14221 ttataactca ctaaaaaatg gtatagaaaa accttggata cttaatactg gaaaataatt
    14281 ctggtaaata gaaagattac tttaacatac gtttgattaa aaaatagtta tgcacactcg
    14341 tggctttagt catggaagat tcagaaggca aataatagaa atagattagt aaaaatatat
    14401 ttacttataa aactgtatac ttttatagag gctatctcaa aattggatat gtccctgtca
    14461 agtagacaga cttaaaaaag gctatcttta tgcggaatga gttcgatatt caatcgaact
    14521 cattcc
//
LOCUS       DAMKPG010000092        14361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMKPG010000092 DAMKPG010000000
VERSION     DAMKPG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.097"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            358..2592
                     /locus_tag="PC621_002917"
     CDS             358..2592
                     /locus_tag="PC621_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4110062.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2623..3468
                     /locus_tag="PC621_002918"
     CDS             2623..3468
                     /locus_tag="PC621_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF4110063.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3708..5441
                     /locus_tag="PC621_002919"
     CDS             3708..5441
                     /locus_tag="PC621_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF4110064.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5767..6519
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5833..5861
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(7050..7406)
                     /locus_tag="PC621_002920"
     CDS             complement(7050..7406)
                     /locus_tag="PC621_002920"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF4110065.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIETYIYYYNNFRYQKRLN
                     SMSPLEFRKYLSTEIA"
     gene            7502..7995
                     /locus_tag="PC621_002921"
                     /pseudo
     CDS             7502..7995
                     /locus_tag="PC621_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8537..9892
                     /locus_tag="PC621_002922"
     CDS             8537..9892
                     /locus_tag="PC621_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4110066.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(10010..10678)
                     /locus_tag="PC621_002923"
     CDS             complement(10010..10678)
                     /locus_tag="PC621_002923"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF4110067.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(10745..11173)
                     /locus_tag="PC621_002924"
     CDS             complement(10745..11173)
                     /locus_tag="PC621_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF4110068.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            11280..11873
                     /locus_tag="PC621_002925"
     CDS             11280..11873
                     /locus_tag="PC621_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF4110069.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            11993..12928
                     /locus_tag="PC621_002926"
     CDS             11993..12928
                     /locus_tag="PC621_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF4110070.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(13199..14143)
                     /locus_tag="PC621_002927"
     CDS             complement(13199..14143)
                     /locus_tag="PC621_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF4110071.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         5434 a         1947 c         1829 g         5151 t
ORIGIN      
        1 aataaaataa ttatgccttt gaaaaatcat taaacttaaa attaattata aaaaaactct
       61 accttactaa ctatacagct cacaatctat ctatttatga aaaaatatga tataattgca
      121 ttcaaaaagg aaatctttca atatactata atttaagcat taactaattt tatctaatat
      181 tattactaaa ctaaaaaata tttgtatttg tagcattgga gcaaataact taatgtatat
      241 tcattttaaa tacttattta aaatttagca tttaaattat ttacaaatta acatttaaat
      301 acttctagca taaatacagt tgtcatcaaa atttaaagta tcttatggag gaataccatg
      361 caaaatcata attatataaa aatgttaaat aaaaaggtag aagtattatc taaggaatta
      421 gatagtagaa attttaaatt aaaaacctta aatgaaatag attttttttc taaaatacca
      481 aacagagaat ttatgtacca atatattgat gactgtattt cgaaaaatat acatatagga
      541 atcatgttat taaatatcga taactttaaa ttatttaatg atatatatgg atatctagtc
      601 ggagaaaatt tattaattac tctaactcaa aacctattgc actttattcc agaatcaaag
      661 ggaatagtcg cttatttaaa aggagaaggt tttttaattg tacttccaaa tgtatcaaat
      721 aaagagttaa ataatatggg tgcaactatt atagagagct cccgagaatt aaaaataaga
      781 cttcctaatg aaaaagaaat atactccaat gttacattta gtatcggcag cactatctgg
      841 aatggtgaac caaacatgac tactttgata cttttcaacc aagtatatag tgctttaaaa
      901 aaggctaaaa gtgaaggcaa aaatagatac attctatttg attttaaaga taatagtttt
      961 tacaataaat tagaaaatga taaggagttc ttatcagaaa tatcacatgt tctacaaact
     1021 aaacaaattg aagtatttta ccaaccactt tataatataa aaaataatac tatagttgga
     1081 tgtgaggcac ttgcaagatg gagacaccct attaaaggac ttcttactcc agataaattt
     1141 attcctttat ttgaacagtt tggtctgata acggaattag atttgcattt atttgaagag
     1201 aactgtaaaa atataagaag atggattgat aacaatgatg tttttgttcc aatttgttgt
     1261 aacttttctc gtcttcattt ttttaaccct aattttccaa gtatgctaaa aggtattact
     1321 gaaaagtaca gcgtctctac atcaaatttt agcattgaaa taacagaaaa tatattaata
     1381 gaaaatacaa atacaattat tcaacaacta gctgaacttc gttcttatgg tttttctgta
     1441 tcaatggacg attttggttc tggatattca tcttttggta tgttgcaaaa tctacctttt
     1501 gatataatca aaattgatag gatattttta tctcataatt taaatgattt taaaaataca
     1561 accattttgt actctataat aaacattgca agagctcttg ggatgcttac agtatgtgaa
     1621 ggtgttgaaa cagaacaaca tgtagaattt attaaaagta taaattgtga tatcgcccaa
     1681 ggttattatt atgcaaaacc catggagtgc aaagaatttg aaaaattact atctaaagag
     1741 aataattcag agaaaatgtt tataaaatat tctgatatag gaaaatcttt aatagagaag
     1801 atatttgatg gattttttat tatgcaagat atgaaaaaac taagagctaa tgtcactgaa
     1861 gacattcaat ggtttgattt ttacaacaaa actgaaatta catcttttcc taaagtaata
     1921 gaacattttg aaaaacatat taaaggtaaa aaattttcta ttctttacaa aagtataacc
     1981 ccacataata ctgaatcaga ttcattatta attagtggag aagccgtttt atcagatgaa
     2041 gcttctgaac atcaaaaata tagcaatttc tactttagtg caaattgtat aatttcagaa
     2101 aataaattac tcttagcaaa attacatttg gatttaatta aaactgctgg ctatataaat
     2161 gaatttgaat catattcaat acctaaaatg acaaatacac atcttgatga aagtaatatt
     2221 ttatcacaat tttattcaat gttgcctgta ggtataattc gctacgattt atttggtgat
     2281 atgattatta cttatatgaa tgaagaaatg tttgacatta taggttatac aaaagaacag
     2341 ttttttggtg agatgggcgg aaatttaaga gcaattgttc atccagatga tttagacttt
     2401 gtttataaaa aaagtttgga tatgattgac acaaataaaa ttgaaccatt tttatataga
     2461 ttcataaaac gagatggtca aattgtcaca gttatgtata cacaatgtaa cctttctgca
     2521 attgatggaa ggtcgattgc ccaaggtatg tatgtagatg taaataaaat aaaaaattta
     2581 acaaaaatct agtacaaagt attttatttt ggagaaatta atatgtttaa agaaattttt
     2641 ttaagaactt ttccaggatt tctaattata agagattcta actacagaat tatatttata
     2701 aatgataatt taaaaaattt aattaaaagt tatatgcaag ataatccact tggaatgact
     2761 aatatagaaa tagctaagaa actccctgat aatattgcta aattttttac ggattctcac
     2821 aatattcaat tagattggga aaaaaattat ccttatgata aaatttcaaa ttggatttta
     2881 gagtttaaaa aagatactac aagctattgg aatgttttag aatacaaagt agatgttgat
     2941 gaaaaaacct acataattac tatggccaat gatataacaa aactctatga agaaaataag
     3001 agaaatcttc actactcaat tactgaccca ttaactggag catataatag aaaatattta
     3061 aatgatagat ttgacatatt tataggagat tatattgtac taattgactt agataatttc
     3121 aaaatgataa atgactatga aggtcataat gtaggagaca agattttatg tgattttgtt
     3181 tcacttttaa ataaagaatt aataaattct acttctatta taagattagg aggagatgaa
     3241 tttattgtta tattctctag tgatgttgac aaaaattatg tttattcaca actagaagcc
     3301 ttaagagaaa attttttaaa agtattctct aaatataagt atctttcatt tagttatggt
     3361 gttgatacag taaaaagaaa tttaaaattg acaatagttg aattggataa aaaaatgtat
     3421 aaaaacaagg agaaaaacaa gaaaaaattt gataaaaatg attattaaca ttgttatttt
     3481 aaaataaatt aaaattatct ttcttttttt aaacttgtac attagtttat aaatatgtat
     3541 ctatacaaga tattgacatt aagtactgaa gcatatattg tttcagttct tttttgttaa
     3601 aattaactta tttgacaaag atatcatagt ttaacaaagc ttttaattta aatatattga
     3661 tgtataatat aattaaaata taatagcaaa aagaaaggag gaatatcatg agcatactcc
     3721 taaaaaaagc acccaaatta gcaaaacata ttataacaag tttttatata aatcgagata
     3781 tcaatgaagt attaaaatac ctttgtgaaa atgttacatg gattggacca ggagaacagg
     3841 aatttctcac ttcttttaat gaaataaaaa attattttta tgcaggtcaa gatgaaattc
     3901 cttcttgtga tataaataat gatgtatttg aaactgtaag tgaagatgag aatagatgta
     3961 tggtattagg aagatatact gttcgaacta aagaaaatgc tcaaatgatt ttagaggtaa
     4021 atcaacgttg tacatttgag ataattgagg atagaggaaa attattagta aagcacatgc
     4081 acatatcaaa cccttatggt gaaatgcaac ttgatgaata ctttcccact aaaattggaa
     4141 ctcaaagtta tgattatttg caaagattac taaaggaaaa aacagaagtt attgaaatga
     4201 ttacaaataa tatcaatggt ggcttaaaag gtagcaatga tgatagtaca tactcatttt
     4261 tctatgtcaa tgagggattg cccaaaatat taggatacac atatgatgag ttcatggaaa
     4321 tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga cttacctaaa gcactagaag
     4381 attgtgaacg atgttttgca aaaggaccta catattcatc tgaatataga atccgaaaaa
     4441 aagatggaac attaatgtgg gtattagatt ctggaatgaa atcgttaaat agtgatggca
     4501 tagtaaaaat taatagtatt attactgaca taacccaact aaaaaacata gaatctgaac
     4561 taaaattaga aagagagcga tatcgtattg ctttgcaaaa tattacagat atcatgtttg
     4621 agtatgatat tgaaaacgat acccttataa aatatcaacg tgtagaaatt gacaaaaaaa
     4681 ttgaattaga aaattttgaa acaaaaaatt actccaaact attagaatct ggtaaaatta
     4741 ttcaccttga tgatattgga aaactactag aagtacttca tggaaatctt cgtgaaacaa
     4801 ttgagataag acaagtaaat tcattaacta aaaatgaatg gagatggatt cgtgttcaat
     4861 gttcagttat atatgatagt gaccataacc ctatcaaaac aataggcgtt ttaaaagaca
     4921 taactgaaga taaatctaaa ttagaaagct taattaatca ggctcaacgt gacccactaa
     4981 cacaattata taatcaaaga gtatcacaaa atcttattga agagtatcta tgtagttctg
     5041 attccaaaaa taatgatgct ctattagtta ttgatattga tgattttaaa actgtaaatg
     5101 atacttttgg gcatttagag ggaaatgaag ttttagttgc tgtatcaaaa atactattac
     5161 ataacacaca tgataaagac atagttgctc gaattggtgg agatgaattt acaatattta
     5221 taaaatcatt aacaaaggat ttaataataa aaattactaa tgatatttta aatgatgcaa
     5281 gtaaaataaa agtaaaggac aatcataaaa taacattaag tattggtatt gccttcactg
     5341 atgattcaac taaattatat aaagatttat tttctaaagc tgataaagct ctttatctat
     5401 caaaagcaga tggcaaaaat tgttatagta catacgaatg ataaagctat ttatacgacc
     5461 aagaatgaac ctttagattt aatttcatag tctaacaatc aaagagttga agctaatctt
     5521 atagctagtc aactagttcc tagaaaaaat ttagacaata tatttattga tactgctcat
     5581 ttttctaaag atcaaattta tataaaaaat taaactttta tgtacataca gatactaatg
     5641 ataaacattt agcattagta tcttctatct taaaataaaa tgataattta tctatataaa
     5701 aaagtgcttt gttgacagag ttcacttttt tatataggta cagaatttaa gtataaatac
     5761 atgttttatt ctatacattt tagttaatgt aaaatttctt gattgtactc tctaactttt
     5821 caacattata ctatttacat accacttagt taatataaaa ccaaaagttg gagaaataga
     5881 agtgccattc acattcgaat ttacatacca cttagttaat ataaaacact ttaataattc
     5941 tacctttact agtgctaaag taatttacat accacttagt taatataaaa ccaggaacat
     6001 atggcaaact ataaggaaaa cctgcaaatt tacataccac ttagttaata taaaaccctc
     6061 tgtgactcca tattttttca taatatataa ttcatttaca taccacttag ttaatataaa
     6121 acatagaagt tgttgcatat aactacattg acaagttatt atttacatac cacttagtta
     6181 atataaaact tttctacttt tttaatcatt taatcacctg cttaaaaatt tacataccac
     6241 ttagttaata taaaactttt gatattctaa agaagtgaca atgatgtgac acaaatttac
     6301 ataccactta gttaatataa aactattatg tagtcatata tttcaaactc taatttcgta
     6361 tttacatacc acttagttaa tataaaaccc atttgaccca tgcatattga cttccacctt
     6421 ccttatttac ataccactta gttaatataa aactttacaa tatagacgta catatacttc
     6481 tgaaaaacct ctttccatac cacttagtta atataaaact ttaccccaaa ataaactggg
     6541 cattttcaat acttacacat gtgcaactct cttaaatttg cagtgaacca tgagtagtgc
     6601 aaatgataaa gtttatcata taccctcaac accttatatt tcaagcatta agccctactt
     6661 tgtaacaaat atcgctcact gcaaaactat ttacattttt attatatcat aaatatatta
     6721 tttttgaata tctgtaccaa tttgtggtat aataaaaaca agaagaacta caatctattt
     6781 agacgtagag tgaagttcaa atactaatgt ttattctttt tgaacttgat ttttagttca
     6841 aaactaaagt cactcctgct gaactatacc cactgtttta tacagttaaa acatgtaaaa
     6901 gtagttataa agtactagtg taactcatta gtactttttt tattatattc atataattaa
     6961 tatctatatt ttaaagttta ctatctaaaa aagagcacgt caaataaaac cctagatatt
     7021 taaatttttt tagagctact taaaataaat tatgctatct ctgtacttaa atatttccta
     7081 aattcaagtg gagacataga atttaatctc ttttgatatc taaaattatt atagtaatat
     7141 atataagtct ctattatttt aactaattgt tcatatgtat caaatttcct taaatagtac
     7201 atttccacct ttaaaattcc ccaaattcct tccataggag cattgtcaat acaacgacca
     7261 gctcttgaca tactttgtat cataccttgt gctaaaagtt tcgattcaaa caccttacta
     7321 gtatactgaa aacctctatc actatgaaaa acaggttttg catttggatg tagttttatt
     7381 actttatcaa aagtttcgaa taccaagtta ttattattcg attttccaat aacatatgaa
     7441 attaatagat ataacgcctt ctattttaca gtcttatata aataacatat acaatgagca
     7501 catgttaaat tcaataaaaa accaagtcga ttttataaaa tcagtattga aagaatctta
     7561 tagattaaaa gaaatatcag aaaatatttg tgattttgtt acaaccccaa tcaaaaaaaa
     7621 tagttcaact tctaagtttt atacaaagca agaaactcaa ttattattag aaaaatcaaa
     7681 aaatattcct ataggtatac caattttttt gatactaact cttggattac gttttggtga
     7741 agctgtatca cttatatggt cagatgtaga ccttgataaa aaatctcaca tgtaaatcaa
     7801 acattagttt atgtatacaa taaaataatc ttcaaagacc ctaaaatccc tagatccaaa
     7861 agaaaattat ttgctcctga tgaacttata agtttgctta aagggcaaaa attaaaacaa
     7921 aataagttaa aattacaggg tatattgaaa aatgaatttg atttgatatg cttaaataaa
     7981 agattcaacc cgtgagttca accaactttt gttgttcatt ttaaaaaact attaagagat
     8041 aataatccta gagatataaa agtacatgaa ttaatacatg caaatgcgac tttgatatta
     8101 ctttcaggta ctaacataaa agctatttat aaaatattag tacatgcaga tataaaaata
     8161 gctatgaata aatattcgat attctcactt attagatgaa atggataaag aaacatctga
     8221 aaatataagc aaaatactat tcaaataatg ttcagtttta tgtcaattaa atgtcactca
     8281 aagtgctttt tttccatttg tcagttaaat gtcacttaaa gtaccttttt cctatttgtc
     8341 agttaaatgt cagtaatata taaataagat atatttataa gacccttaaa atcactatta
     8401 tttaataaaa tttataatta tatactattt gctgttgaca ttggtattat accatagttt
     8461 atcatttata ttagaaataa aattattaat taatagtttc gtaattttat aatagattta
     8521 cagggagtga taataaatgg atacaaaaat atctataaac gaaaacatga gtctaggtaa
     8581 aagatttttt aaatacctag caccatctgt agttgcaatg tgggttttct cactatatac
     8641 aatggttgat ggaatatttg taagtaaagg agttggagaa ttggctttag ctgctgtcaa
     8701 tatatcaatg ccttttatta attttatatt tgcagtttct ctattatttt caactggtgc
     8761 atctaccata attgcaatct atctggggaa aaaagatatt aaatctgcta acgaagtctt
     8821 ttcatttaac ttagtatcaa taattatatt atcaataatt atattagcta taactttttt
     8881 taatttagat agattagcac tatttttagg agctacagaa tctacaattg gtatggttaa
     8941 agattattta ggaataataa tattttttaa cggattcttt atagtgtctt attctttaga
     9001 agttataata aaaactgatg gctttccaat attagctact gttggtgtaa taatatctgc
     9061 tcttacaaac atcatacttg attacttgtt tgtaattgaa tttggatggg gtgtaaaagg
     9121 tgctggtatt gcaactgttt tgtctcaagt cttttctact attttcttct taatacattt
     9181 tttaagaaaa aactctactt tgaatttttc aaaatttaga attgatttta agacacttag
     9241 aaaaattgta tttataggtt ttcctgattc aactactgaa ttaagttgtg gtatagttgt
     9301 tttacttttt aacttaagtc ttactaaata tattggagaa aatgctctta tttattatag
     9361 tgtcataaat tatataaata cattagtact tatgactatg atgggtataa ctcaaggtat
     9421 gcaaccactt actagctttt attatggtgc aggaaatata gataatgtaa aaaaattatt
     9481 aaaaatgggg ataaaagcca caataatagc atcagttgct gtatttgcaa tttgtatggc
     9541 tttttcagga ccaatagtct ctttatttat acacccagaa gaaacaatgc tattcaatga
     9601 aggagttcgt gtatttaaaa tattctctat atctttctta ttggtaggaa ttaatgtaat
     9661 aatttctgga ttctttgtat cagtagaaaa accatcaatt tctactgtta tatctcttgg
     9721 tagagggctt gtaatagttg ttttaagtct aatttcaatg atactaatat ttggtggaca
     9781 aggaatatgg atgactacta ttgtttctga gtttatatgt cttattttat cattagtttt
     9841 tctaaaaaag aatttttcta cattagactc taatctaaat aaggttgctt agatgataag
     9901 agtagcaata tatgaaaata tctaaataaa aaatacctat tatacaatct tgcaaatatt
     9961 agacctttgc aaaaattagg attatataat aggtatttta tttatgtaat tattttattt
    10021 ctaaatatcc atagccatct tttattaaag ttccttttac ttcatattct acaggttttt
    10081 taaatattgg accatctacg acaaatgtat gatactctcc atgttctcca catgggtctg
    10141 caccagcatt ttttatcttc tccacaagtt ctctagttaa tttttttcct aaaaattcct
    10201 ctcctaagtg ctttaagttt acttttgtta caacactaca aaatccactg tctataaatt
    10261 catatactaa atcctctcta ctctcttgcc ataatggtaa atctactttc ataccaactg
    10321 cttcacatct atcagtatcc cattttctat gactttctat atctatatct ccatatgcac
    10381 atatcgttgc tcccatgttt ttagctttta ttaaagccct ctcaaagctc tcttcatatc
    10441 cttctacacc acactcagca actaataaag gtaatcctat attgctactt acttgcttaa
    10501 gcatattata ttctaaatta tgtgtccatg actttccatg ttcttcactt atagttgtca
    10561 agagtgccac aggcttatat ccttttttca acattcgatt taatgctaat atactatcct
    10621 taccaccact aaatgacatt acaaattttt catcagccaa ttttaacacc tctttcatct
    10681 aactttgttt tattaacata attataccaa atttttgagt tgcatagcaa attatattaa
    10741 attgttaatt cactataaag aatgtcccta atattaaaag tataacccct atgtatttgt
    10801 ataaatgcat tgtttcttgt cccaaaatta cgctatcaat aaaaaatcca actaaaactt
    10861 gggatacaag tgttaatata agtgtatttg atactcctaa atttggaatt gcctttactg
    10921 taaaatatat tattaaacct ccaaatactc ctcctaataa caacttataa ttaaaaccat
    10981 ttatagatac tagacacttt aagtcacctg taactattat acttgccaaa aaaaatattg
    11041 ctcctacagt taaacttata aaggtggcca ctaatgctgt tgtacttttt cctagttctc
    11101 catttataaa agcttcaaga gctgtagaac cacctgctaa taatgcaaat attattgccc
    11161 ataattttgt catggacaaa cctccttcta tataattcta ttaatagaag tctatgatac
    11221 aacctattaa ttaattacta agtatagttt aatttttata cactaggctt tatttggata
    11281 tgaatttctg ttataattgt tctatcgatt ttggaggtga ttttatgtac acaaatttta
    11341 atatcgattt taataacttt aataaaaaag aaaatgctac taaatttatg ttaatgggag
    11401 tattgctcat tatcttagga ttattatgcc ttacatttaa gacattaggt ataaaattaa
    11461 tttcttggac ttttggtatt gcacttttat tctttgcata tttaaattta aaaaatatta
    11521 atgaactaaa aagatatgct actaaagaag aaattaaacc ctctattaat atccaatggg
    11581 ttctaataat tgcatgtata ttactttttg tatttcctca aaaaatacaa agcatatttt
    11641 cattattttt agggttttat ttaatcttta atcaactagt agcacttgta aatagtaaga
    11701 ataatcctta ttctaaattt acaacttgga atatagttaa gattctattt ggtatatgcc
    11761 ttatattatc tccacttttt ttatctagat ttatagtatc gataatgtct tttttcataa
    11821 tattatttgg actagttcta tttttctctg gaaatacagc gagaaaatat taattatttt
    11881 ctcgctgtat ttttatatta cttaaaatag aatttgttta agttatctat ttgtggtaat
    11941 atgttttttg tacaagctca gcgtacaatt ttattagaaa taggtgattt ttttggataa
    12001 atttaaatat gcttttgatg ataaaagata tcatacttgg aattactatc ttagaaatac
    12061 ttttggagaa aaagttttta aggtatctat aaatgcagga tttacctgcc caaacattga
    12121 tggaagtctt ggttatggcg gatgtaccta ctgtagcaaa gaaggctcag gtgattttgc
    12181 tggaaaccct aaagataatc ttatcagtca attttataat ataaaagaaa tgatgttaaa
    12241 aaaatggcct catgcaaagt atattggcta ctttcaagcc tatacaaata cgtatgctcc
    12301 tcttgaagtt ttaaaagaaa aatatgagac tatattagaa cttgaagatg tcataggtct
    12361 ttcaatatcc acaagacctg actgtttgcc agatgatgtt gtagaatact tatctgaact
    12421 aaataaaaga accaatttat gggttgaact tggacttcaa acaattcatg attctacatc
    12481 taaaattata aatagaggtc atgattataa aacatttgtt gaaggtgttg aaaaattaaa
    12541 gtctaaaaat ataaaggttg tcgtacacat tataaatggt cttcctggtg aagattataa
    12601 tatgatgatg gaaacagcaa aggcagttgg taaaatgggt gtagacggta taaaaatcca
    12661 tctattgcat gttataaaag atactcctat ggaaaagatg ttacaaaatg gtatgttaac
    12721 tcttatggaa caagatgaat atatcaatct tgtctgtgac caattagaaa tacttcctga
    12781 aactatgata atccatagac tcactggtga tggtaagagg gatgaactag taggtcctat
    12841 atggagccta aaaaaatggg aaattttaaa tcaaattgat gatactttaa aggctagaaa
    12901 ttcttatcag ggctgtaagt ttgtttaata caaaataata actatagaaa tggttatata
    12961 aatatagtcc tttctatagt tatttagtat ttacaaaaca aaatattaat ttgtacattt
    13021 atacaatata taataaaaaa tagatagtct aaactgtatc tacttatatt ttatttgtac
    13081 tgtccacttt attctataaa atttataaat agttattttt taataccaaa tataatttgg
    13141 tataattttt gttggattct aaaatgtata ccaataaatc ctttttaagt atataattct
    13201 aaatactttc agttttttca atctgcttta acttgttgga tttataaaat attcttattt
    13261 catcataaga aagattaagt gctactactg cagatattaa catcaacatc actccaaata
    13321 acttccctaa ggaaaaactt tctcctaata tagtgcaacc aattattact gaaccaacta
    13381 actctactga tgccacaaca cctgcaactg acaactctac acctttagat atacctgctg
    13441 cataaaaaat atatgcaagt gcagctggaa caatgcctaa cattagcata caagataaaa
    13501 catctaaatc cttagcataa tttaagattt cccaaggtct cgaagaagga atcataaata
    13561 tagctccaaa taagaaactg tatactaata ttgttgaact tgaaaattcc tttaaagcat
    13621 ttttactaat agttggcata agtgcatatg ctattgctga taatacccca agtaaaagtc
    13681 caaaagcgtt taaaccttga aaatccaaac gtccaccagt cactgccata attgcaccaa
    13741 taaaacaaag aataagtgaa aataatttgc ttctagtaat attttcttta taacatactt
    13801 ttgaaaatat tgctaaaaac agtggtgatg tatatagtaa aactgctgca attgatacac
    13861 ctgcaatgtc aattgcttta aaataacata aattaaacat tgcctgacat ataattccta
    13921 ttattactga atatataatt ccttttttgc ttatctttaa gatttgtggt gtccttatag
    13981 atgaatatac gcctaaaact ataaatccaa aaaacaatct tgtaaatgct acttgttctg
    14041 atgtcagtcc atttcccatt aaaaactttc caaataatcc aagtgtagcc cataataacc
    14101 ctgctattgc tataaataca taccctttac ttttttctcc cattttaaat tcccttctat
    14161 ttttatttag tcaatttaca ctcttctttt taagcaaaaa ttatgccatc ttaaaccaat
    14221 atatatcgac tctaaaaccc tagtctttcc aataatacta ataagttttc aattttctta
    14281 tatttatttt tcgcttactt taagttgatt tagacttatt ttaattgtaa aatgagttat
    14341 atataactta tttaaagtta a
//
LOCUS       DAMKPG010000093        14041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMKPG010000093 DAMKPG010000000
VERSION     DAMKPG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.098"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            129..629
                     /locus_tag="PC621_002928"
     CDS             129..629
                     /locus_tag="PC621_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110072.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(879..1658)
                     /locus_tag="PC621_002929"
     CDS             complement(879..1658)
                     /locus_tag="PC621_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110073.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1655..2134)
                     /locus_tag="PC621_002930"
     CDS             complement(1655..2134)
                     /locus_tag="PC621_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4110074.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            2940..3641
                     /locus_tag="PC621_002931"
     CDS             2940..3641
                     /locus_tag="PC621_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF4110075.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            3667..4323
                     /locus_tag="PC621_002932"
     CDS             3667..4323
                     /locus_tag="PC621_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110076.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            4530..6170
                     /locus_tag="PC621_002933"
     CDS             4530..6170
                     /locus_tag="PC621_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF4110077.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            6529..7851
                     /locus_tag="PC621_002934"
     CDS             6529..7851
                     /locus_tag="PC621_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110078.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            8048..9055
                     /locus_tag="PC621_002935"
     CDS             8048..9055
                     /locus_tag="PC621_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF4110079.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            9093..10490
                     /locus_tag="PC621_002936"
     CDS             9093..10490
                     /locus_tag="PC621_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF4110080.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            10635..11900
                     /locus_tag="PC621_002937"
     CDS             10635..11900
                     /locus_tag="PC621_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4110081.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11942..12981
                     /locus_tag="PC621_002938"
                     /pseudo
     CDS             11942..12981
                     /locus_tag="PC621_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            13016..14038
                     /locus_tag="PC621_002939"
     CDS             13016..14038
                     /locus_tag="PC621_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF4110082.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         5041 a         1614 c         2641 g         4745 t
ORIGIN      
        1 ggttttaatt ttatagaaat atacaaagat attttaagct aatatatagt aatttatata
       61 gcattataaa ctagaatcat ataaaataag ttaactacaa tcataaatta aaaaggagaa
      121 atgttaagtt gatagaagga ataaaattta taaaggcaga agaaaagtat atttattcgt
      181 attggcaaac ttttgataaa atagcaaaag agaggaaata tctagctatg gatgaagcct
      241 ttccttttga agaaacagtt gaatttataa aaaatactat aaataaaaac ttaccacaac
      301 tgtttataat tgatttagaa tcagataact gtattggttg gtgtgacgtt ttacctaaaa
      361 ctgaaaaagt tggttattta ggcatgggta ttcttaaaga gtatagggaa aaaggtatcg
      421 gaagtagtct tttaaaacaa attattgatt tatcaaaaga atatgggtat gaaaaaattg
      481 agcttgatgt atttaaaagt aatagtagag ctattcatgt ttataaatct ttagggtttg
      541 tagaggtaaa tacaatatct agtggtttta catggaatga tagacctgta aaagaagaag
      601 taattcaaat ggagcttaca cttatataaa ataaagttaa agtctttaaa attccatata
      661 cacttgtata aaataaagtt aaagccctta aaattccata taaaagaaat atttttaagg
      721 gctttttaat aatggttctt ttgaaggtag ctaaatatct aaaattttat atattaaatt
      781 taaagaaaat ataaagatgg aagatacaac catgtttact aataaaaaaa ttaaagtcat
      841 aaataataaa ctcataaaca acttattatt acttatgttt attttgcatc tattacttca
      901 tgagccattt taactaagtc attcatagat aaatttgagt cttgaactgt aaaagaatag
      961 taaactccat cttgtttcca tgtcaaaaac ttaaattcat taacttcaac tttgtctgat
     1021 tcattatttg gccctacact aaatgtgtat ttgcctgatt tttcatcaat tttatcctgt
     1081 tctgtcattt tataaccttc tggtacaaac ttttgtgcga atgcttcata agaaatatta
     1141 atatctttgt aagtatctac aacttttaga ttgtcagaac tcttctcaag tacattattt
     1201 gctgtgtcaa gagatatttc atcattgcca tttttatatt caaaactaag tgcttttgat
     1261 tttcctaaaa cattattttc tttgtcaagt gcttttttat ttacagtata tgctccttta
     1321 aatttatatc cattactaaa ttcagaaact atattagggg taaagttaaa gtcttttttt
     1381 acttgttctg tagttgggat agatgtatag gtagccttag tacttgattt tccaactaaa
     1441 ctatatatat gtcctactgc aagggctgtt gtgctcaata tcaaagtaat ggctaaagtt
     1501 gcaactaagc cttttctaaa tggtaatttg tgtgttttat tattaatcat tttataattt
     1561 tcctctcttt ctggtaaact atttagtgtt ttacttaaac tattatgaaa actttctgga
     1621 acttctggaa acgaattatt taatatattt ttattcatat gattacctcc gtgttttcta
     1681 atttagtttt taataatgtt cttcctcttg ataatctact ttttacagtt ccttgaggta
     1741 tatctaatat ttccttaacc tcatctactg aataaccttc aatatagtaa aggactattg
     1801 gaattttaat ttttttaggt agactttgaa tagcagtata caaatcgcta tagtcatctt
     1861 tttcatcaat ttttttgttt ttgaagacat cttcaaatga tagagatttt aatcgtttgc
     1921 gtcttaatga attacattca tttatgacta ttctaatcag ccatgtttta aagtattgtt
     1981 cttccttaag agaatccaac ttttcatatg cttttaaaat agcattgttg acggcatctt
     2041 cacagtcttg gtcgtttcct agaatagact tagatactcg atacaaagtc tgttctgatt
     2101 caagtatatt gtttgtaaat gtagttttat ccaactgagt tccttccctt ctatgcaaat
     2161 cgtagcgcta cttttttgcc tttcacttat tagataaaat tttactatag atggttcact
     2221 ttttttatga ttatttgata aatttaagtt tgaaagtttt gcctattcta tacaaatatt
     2281 ctataatata tttttttcta gtattttccc ctcatatata aagccatgta gatatattaa
     2341 actttagaag ccatttatat atttagtttt atagtaagtg tttatattaa gtaattataa
     2401 aatagtaagt atttgatgtt gagtagttag aaaataatgc ttttggatta tttacttcat
     2461 gtatagattt aaaataagtt ttttaattaa tgactaaaga taacggtatt ttgactaaaa
     2521 gtaggctttt aagaaaaaac aatgaaactt aaatgagaat caagagttag aaatgtcatt
     2581 attttagata gatatgaaca taaataaact taaaaaatat aaattataaa aataataatt
     2641 tttttaaaat aacaaagttt tttgattaaa agttttatat tgaaatgtca aagtaataaa
     2701 ataccttata agttaattac aggttaaaaa aataatatgt gaattttctg atatttacta
     2761 attatcaata aagtatttaa gtattaatgt ttttagtgag actttaagac agataatctc
     2821 ctattaaaga tactatatct tagataaaat aaattaaaac aatataaaaa cataacaaga
     2881 ttacagaaag aaagctcata ataaaacact aaaaaattta ttttaaggag gaataaagta
     2941 tgaagtttca agaaatgatt aatagcccag gtctttggat tgtttctagt tttttagtaa
     3001 ttatttcagt tgttcaagca atcgtattta tgaaatcagc acttaaagag gcttcaaatc
     3061 ttggtattga aagaaaagga attaaagcag caattcgctc agctagtgtt acagcaattg
     3121 gaccctctct atctcctgtt attacattat tatcactggt agctgtgatt ggtgctccta
     3181 cgacttggat gaggctttgt gatgtaggag ctgccagaac agaacttgga gtaatatctc
     3241 ttacatcaaa tttatctgga gtagaagtag gtagtgctgc atttggagca gaagcttttt
     3301 cgtatgcact atggggaatg gctttaaata acttaggttg gttgcttgtt gtatttattt
     3361 tgggtcatag aatgcgtgga attgttgaaa aaatgaatgt gaaatataat cctacttggg
     3421 ttaaaaaact acttgcaggg gctacagttg gattatttgc ttaccttctt tcaaatcaaa
     3481 taaaaacatt cgaaatacca aaacttacac cagcagttat ttcagctata gttatgctag
     3541 tattgacgac tttatttaaa aagaatcaac gattacaaga attatcctta ggtattgcca
     3601 tgttgattgg tatgtttggc acacagattg tactatcatg attaaaacag ataagaggtg
     3661 ataattatgt atgataaaaa gatgataaaa atagggagaa tttctatagg tcttgcaatt
     3721 attgcaaatt ttattcctgc aatctatgta ggtcttcgct ttggtcagat gccaatgctt
     3781 tctgttatat tccaaatttg gggattagtt gctacaactt atggagtgag ttggattgtt
     3841 caacctatag cttattatcc aacactagga gctccaggtt cttatatagg ttggcttgca
     3901 ggttctgttg gagatatacg tatgcctgca gcatcaatgg cacagaaaat tgcaggtgta
     3961 gaagcaggaa ctcatgaggg tgaagttgta ggaacaattg gtacatgttg ttcaatcttt
     4021 gtatcagctt ctatgattac tatttttacc attgtaggct ataaagtaat tcctttgtta
     4081 cctgaattta ttactgattc atttaattat atacttccag cattgtttgg ggcaatttac
     4141 gttgatattg cgcgtaaaga tattcgagct ggtggatgta cgattgctgt agcattagct
     4201 attatgtact ttggtgaaaa aattggtata ccaggtggat tgttgacatt gtttattgtg
     4261 ttaggtggga tacttattac aagggttttc tttgtaattg atagcaataa aacgaaagaa
     4321 taggattgtt tactaggaag aaagactaga attatttgct gagaaaaaga ataggattgt
     4381 ttactaggag gaaagactag aattatttgc tgagaaaaag aataggattg tttactagga
     4441 ggaaagacta gaattatttg ttgagaaaaa agaataggat tgtttattaa gaaatatgaa
     4501 tataaataag tcttgaaagg agaagaagaa tgtttgatgt taaaattgcc aatggaataa
     4561 tcattgatgg tacaggaaat tcaagatata aaggtgatgt agggattgta ggagacaaaa
     4621 tcatggctat tggagatcta agtcaaaaag aggctaaaga gactatagac gcaactggaa
     4681 aatttgtatc acctggtttt atagatttcc acactcattc tgatttatct cttgtgtatg
     4741 acaaatacac aagaagccgt atccatacag gtgtaacaac agacgttatt gcaaactgtg
     4801 gtataggagt tgctccgata agagaagaga agaagcagga gttaattgac tatcttggaa
     4861 cgaggataat tggtacaatt ccaataaaat tgaaccttca ctggaataca atgcaggaat
     4921 actttgatta cttaactgaa aattctccag cagtaaatgt tgtagcttat gtagcacaag
     4981 gacctgtacg tatcaatgaa atgggatttt caaaggagcc agctacttta gagcaactta
     5041 aaaatatgaa gttggaagtt agaaaggcta tggaagctgg atgtgtagct ctttcgtcag
     5101 gattagttta tttaccagga gcatatacaa agaaagaaga aatggctgaa ttatgtaagg
     5161 aattgatacc atacaatgga tactatatat ctcatattag agatgagggt gatgaagaaa
     5221 tggaagctct tgatgaagca atttatatag ctaagacggc aggagttcct ctacatattt
     5281 ctcatttaaa agtcatgggt cataagaatt ttggaactat tgataaggta tttaaaaagc
     5341 tagatgaagc tgaggcagat ggcttggaag ttacttttga ttgttaccca tatacagctg
     5401 gaatgacatc tttgggtgca cttttacctc cttgggcttt tgaaggtggt gtagaaaaca
     5461 tggtaaaacg attagaagtg caagaaaata gggacagaat tataaaagag ctagaggaag
     5521 ggattcctgg atggcaatgt ttctaccagt tagcaggtgg ttgggacggt gttgtactag
     5581 cctctgtaat gactgaagca aataaatatg tagagggtaa aactttaatg gaggtagcta
     5641 aaatcaatgg tgaaaatcca tttgatacgt tctttaggct tttgattgaa gaaaaaagta
     5701 aaattcaagt agttgttcat acacaaggtc aagaggatac agataaggtc gtttgccatc
     5761 caaagtcatg tataggttct gatagtatgg acctttctac agaaggctta ctttctcttg
     5821 gaaagcctca tccccgtgca tttggtactt ttggacgaat tttctcttac tatgttagag
     5881 aaaaaggtat gttgactttt gaggaagcag ttaaaaagat aacttatctt tcagcaaaac
     5941 gcttaggaat ttataaagaa aggggtctac ttaaggaaaa ttattttgct gacattgtgg
     6001 tatttgaccc agatacaata gaagataaag cgacttatag taatcctaaa cagtatacag
     6061 tgggagtgga atatgtattg gtaaatggta aaattgcatt ggcaggagga aagcaaactg
     6121 atgtatgtgc tggacgtgta attaaaaatc ctcttagcat agcaaaatag aatacattaa
     6181 gaacttttat aaaaatagtc tttctttgaa tattaaggaa ggctattttt gttaaattaa
     6241 taaagattac tctacttgtg ttttctagag aatttaactt taagtagaaa aaacaatata
     6301 ttaattgtaa aatgtaatta atattaatgg ataagattta aatacaaaaa cttaaataca
     6361 aaaatgtttt aacagatagt cataattaaa aaacttaaag taacttttta tataagatat
     6421 aattgattat gcaaaatttt atgaagcagc aaccttgtta ctttaagtca agggaggttc
     6481 agaaagtgtc ttaagagata atagtatctt taggaaagga gaaacgaaat gaaaatactt
     6541 tatgtaggac caggcatggt aatcgacaaa atactagcag taagtaaaaa gaattttccc
     6601 aatattgaag cagattttct tagatatgat agatatattg aagtacctga tttattggaa
     6661 aaatatagag gtagagtaga tgcagttctt tttacaggta aatcgccatt taaatttttt
     6721 gaaaaaagac aaaaagattt tgtgttgtct gattatattc ctagacatga aacaacatta
     6781 tacagagtat tgcttgaaat tacatatttg cttaaattcg atattagtag acttagtata
     6841 gatacttatg ataaagctat gctgaagaga ttgtataaag aaattggagt tgaatttaag
     6901 gatgaacata tgttttttgc agagcaaaag tatttagaag aggactacat aaagtttgtg
     6961 cttgaatttc ataaatataa ttatcaaaat aatgacatat gttgttgtat tacaggtctt
     7021 gaagaagcat atcatgcaat gaaaaaagag aatattccag tagttttagc cttgccttct
     7081 gaggatgtaa tggtacaaag tataaaaaac attcagatgc gttatattgc aaagaaaaac
     7141 aatgagaatc agattgttgt tcttgctatc aaacttaata tgccttcaga atattcactg
     7201 atgaaggaag atgagtacgc ttatctttca caaaggatta agattcttga taaactatac
     7261 catttcaata gccgtataga tggtgttttg gtagagcaga gtagaagcga atttatgatt
     7321 tttacaacta aaaaaatact tgagttagaa acaaagaagt acagagatat ttatttgttg
     7381 gatatgattc gtgaagtttc agttataaac acttatatag gaattggtta tggaaaaaca
     7441 gcaaatgaag ctaaatttaa cgcttatgag agtattaaga tggctcaaag tcaaaaaaat
     7501 gatgcagcat atgtagtttt tgaaaatgga gaaattatgg gaccactaaa gccaaatcct
     7561 gctgtcaaag agaaaaatag ctttgatgaa cgattttatc aaattgctac agagacaggg
     7621 ctaagtgcta atactgtata taaagtgttc aataaaataa taaatagtgg aaaaactgaa
     7681 tttacatcaa aggaattagc tattatatgt ggtgtaagta ttagaactat ggacaggatt
     7741 atacttagat tgtgtgattc tggttattgt gaggtagtga gtgaacagct tatgagcaag
     7801 tatggtagac ccagtagaat attacgtttt aaaccttttc tgttgtttta aatagatata
     7861 agttaaaaat taaattttta tgataacatt ttctttacaa ttttttagat atatgttaat
     7921 atttagaaaa atgcatatgt attcataaaa taaactcaaa atagctaaaa aatcattaaa
     7981 aaggaaatcg tatgcataaa atgagaaagt taatttataa cttaaataaa tctgggagga
     8041 gataattatg ggaaaatatc aggattcaaa aaaagttgta agaaattatt ttgaagcatt
     8101 agaaaatgct actggggatg aggttgaaaa tgtcttaaag caacatatga aatcagatta
     8161 taactggaaa ggtgtgtatc catttcgtga acaagaaggc actgaaaatg tagcagatgt
     8221 attttggaag cctttaaaaa aatcattgag taatatgcaa agacgacaag atattttcat
     8281 tgcaggaaca aatgagattg acaatagtga atgggtaatg agtatggggc attttatggg
     8341 actttttgat aaggattggc ttggcattaa acatactcgt aaaatgatta gtttaagata
     8401 tgcagaattt aactgtgttc aagatggtaa aattgctaaa actggattat ttgtagatat
     8461 aattgggttt atgattcaag caggagttaa tccattgcca ccacaaacag gttcatattt
     8521 tgtatatcca gggccaattg accataatgg attacttttt gaagatgcag atgaagttga
     8581 aggagtaaaa actttagcat tagtaaataa aatggtagat gatttatctg aattaaatga
     8641 aagtggtact atgggatgcc caccagaaat acttaaaaaa tcttgggcta aagatatgat
     8701 ttggtatggt ccaggtggaa ttggtgcttc ttatactata cctagatatc aagagcaaca
     8761 ccagctacca tttagaaata atttaaaagg aaagactttc aatgggcatg tatgtagatt
     8821 tgcagaaggt aattttgctt gtttctttgg atggcctaat ctgactaatg taccatgtgg
     8881 aggtttttta ggattaccag gtggagaggt taaagcagat atgcaagttg ttgatgttta
     8941 ttatcgtaag ggcgataaac ttcaagaaaa ctgggtgtta attgatattc cttattggtt
     9001 gaaacaacaa gggcttgata ttcttgagag aactaaaagt atttttaatg cttagttatg
     9061 catacgtatt ctaaaacaaa gggggatata aagtggcatc taccaacaaa aataatgtaa
     9121 caagtaaaga ggaagaaaaa gaagtttcta caagtgggct tgaatcatgt acggttaaag
     9181 aaaacaaagc taaaatgaat caaactaatt ttagtaatat aaatgaaaat caaacaaatg
     9241 agaataaaac gaacacatat aaaggaaata tagataaaat aaacacaaat aaatccgaca
     9301 taaataaagt taacatagat acagtggata cagataaatt aaatacaaat actcttactg
     9361 aaaaaaataa aaaagttata tttaaggggg aagataatat gtcatctaca aaaatttact
     9421 atggagattc attagaagta acgcctgttg gagtaatgga ttacaatgat ttttcaaaac
     9481 agacaaaaag ggaacaggaa atacctggat ttgattctaa gtatagagat tttgtagatt
     9541 atattatgaa gattacacat aatatctggg aagaaaaagg tataggtgtt atttatgata
     9601 catatcacaa taatgttaca atgcattgtg gctcttcaaa tttagttggt attaaagatg
     9661 ttatttccaa tactcttcaa acattacatg cgtttcctga tagaagatta attggtcaaa
     9721 atgtaatatg gtctaacttt ggtgctgatg gttttttatc ttctcaccgt gttttgtcta
     9781 cagcaactaa cttaggtgat agtaattttg gaccagcaac aggtagaaaa ataaacttta
     9841 gaactgtaat tgattgtgcc acaactaaca atagaatcca cgaagaatgg ttggttagag
     9901 ataatttatg gattgttact caattaggat taaaccctca ggaagtagca aagagtatgg
     9961 caaaggcaag tgaatctaaa gtgcttagct tacaatcaac ttatggtatc tgtgaatcta
    10021 tggatggtca atttatgcca actaagtatc aagcaaaaga tgattctgtt ggcgaaatga
    10081 tgcttgaaat gacaagccgt atttataatt ataaatatat aaatgaagtt aaaaaatact
    10141 atcatgataa tgcagtagta cattttatat gtgataaaga tttaaatggt tatgatgaga
    10201 ttcaaggaat gattgtaagt ttacttgcat caattccaaa tggaagttat gaagtggaaa
    10261 gagtaacttg taatcaaaga gaaaaaaatg aaggttatga tgtatctata agatggagat
    10321 tacgtggaat aaatgaaggt ataggatttt ttggacagcc ttctggaaag catatagaag
    10381 taatgggaat caatcattat catatacttc aaggtaaagt aaaggaagag tggataactt
    10441 ttgatggtat ggatgtatta aaacaaatgt atatgggagt agaagaataa agctgtttag
    10501 agtataaata attttgaaat gtatataggg aagtattgct ttataaaatt taagtgggga
    10561 atgtatatga aaaatagatg tttaaacttt tagcaaaaat tatgactaaa tttaaaaggg
    10621 ggaattataa aaatatgaaa acaacggttg aaatagaaaa tgatatcata ttaggaaaag
    10681 actattcaaa cctaaaaaga ggaattgcat ttttttcaat tgctttaata tacttttttt
    10741 attgctacaa ttttatggtg ggtactttta ttaaaccaac aatgatttat gctttagcag
    10801 atgggggatt tggcttttca ttaaaacaaa cagaagaaat atttgcagtt atgtcttttg
    10861 gtacaattcc aggtacattt atttttgggg tgatttctac aaaaattggg aagaagagga
    10921 ctttaattag cgttgcttta ctgattggat tgacaacatt tataccaatg ttatctccta
    10981 cagatattat gttatggaaa atagcacgtt tgtgtacagg agtagtgttg ggaggcgtat
    11041 ttggaacagc tatgccttta gttgcagata tgtttccaag taaatatcgt ggtaagttag
    11101 ctgctatatt aactgcttta ttttcattag caatgatttt tggtgggaaa gtatatggcg
    11161 ttttaggtga tgcaaattgg caaatattga tgtatactgc gattatacca cctatagttg
    11221 gagctatctt agcaatcttc tttgtgccag atgatttgga gtatacaaag gaattagtta
    11281 aaaagggaaa agaaactggt gaaaagatta gctatataag tatgtataag ggaaaatact
    11341 tatggattgg attgggaacg attcttttat caggtgcaaa ttttacagca tattcagcat
    11401 tttcaaataa tgcaactaca tacctagtga caggaatagg aatgtcagca gcagtggctg
    11461 ggtctatata tagtttacaa ggaattggtc aattagtagg atatttattc tggggttcaa
    11521 ttgcagataa atttggaaga aaaatacctt ttataggtat ggcattagca gcagtatttg
    11581 tttttatata tacaaaatta ggtggagata atacaacaac attctatatg atatcagtat
    11641 tactaggtgt tgctgttgga tattctggag catggggagc atactacaca gagttgtttc
    11701 caagtaaata tcgttcgttg tcatctggta tgtcatttaa tggaggaagg attatatcta
    11761 cgtttgcaat acctgctata gctggtacag cttctggtac tttaggtatg atgactatct
    11821 tttacatatc aatcgcagtc tttgtagcag gtgctattgt atggttattc ttaccagaga
    11881 cgttaaataa taaaaaataa aatactagga atttattttg ttacttagaa aggggtggct
    11941 tatgatttca tcaattgatg tatgtattat aataggatat ttgctattaa tgttagttat
    12001 tggttattat tcaggaaaag ataataaaaa tcaagaagat tatttcttgg caggtaggtc
    12061 aatgccatgg attccaattg ctttatcagt tgcagctact atgattagtg caaatggctt
    12121 tataggtggt cctggatggg catatgtaga tggaatgtat ccagtaatgt aaaatgtgca
    12181 aaaaattctt ttgtagttag tgcatttgtt atgaatttag tttactactt tatgctattg
    12241 gtaggagtaa ttttatttgt tttttatggg ggaaggacat ttgaaacttc aaatgaaata
    12301 atgattactt ttattttaaa tgaacttcca gtaggagcca ttggaattat tattgcaggt
    12361 gtgtttgcag cagcaatgtc tagtatcgac tctatattaa acagtatgac aactgttttt
    12421 acgaaggata tttatgagtt ttatttcaaa aaagacaaca aagaatcatc attaaaagtt
    12481 acaatgatta taactgtagt tataggagtt gttatgacag gttttattat tatgggattt
    12541 ggaggaagca tcaaatcaat tcttgacctt gttggtaact atatctcata ctttgctgga
    12601 ccagctacag gtgcatttgt actagcaatg tttacatata aagctaatga taaaggagta
    12661 tctattggtt ttattgtagg gttagtttta ggatttttta tttctaataa atataatgtg
    12721 agttggttgt ggaatccagc aataggtgct acaataacat tgatatttgg atatattttt
    12781 agtgtcattt taaagaaaaa tcataaagta gatgatatta aaaaatacac tgcatttggt
    12841 atgagaaatc accttatttc tcaaggaatg gataaagtag atggagtatc tatagtgcca
    12901 ttttctttag gtaaacaaga actaattgta ttagcttttt ttattatcca atatattata
    12961 ttatttttga tacaatttta agaagaaata ctagatattt aaggagaaat actaaatgaa
    13021 tcaaaagaaa gttcattata ataaatcggc tacatcaaaa gatgtcgcaa gattggcagg
    13081 agtatctcaa tcatctgttt caagagcatt tggtagtgca aatggaaagg gcgtaaaacc
    13141 tgaagtaaga gaaagaattt ttcgtgtagc aaacgaaata gggtatgttc ctaatttggt
    13201 agcacgggga atgattagtg gaaaaactaa tgttataggt ttgatagttg gtgatagtct
    13261 aggtccattt tacaatagaa ttatcaattt atttgtagaa aaaattcaag agataggtaa
    13321 acagtgtctc gtttttaaag ttcctagaca acaaagtata gatagtataa tttcaaaagt
    13381 aattcagttt caagtagaag ctgtgattat tacagcttcg gccatgaata aggtgatggc
    13441 tgagacttgt gaagaaaaca atattcctgt gattcttttt aatcgtttta tacctggtat
    13501 aaaaataagt acagtctatg tagacccaat tgaaggtggg ggcatggttg ctgaatatct
    13561 gcttaagaaa ggtcataaga atataggata tattcaattt acgagagaaa caagtgaaga
    13621 aatggaaaag aaaattggat tttattctaa gttaagacaa agtgggattc ataaccttaa
    13681 agaagaatct gcaaactatg attatgattc tggatatgaa gcaggtaaga gaatgttagc
    13741 attatcaaat cctccaacag caattttttg cacaagtgac ttgatagcta ttggtgtaat
    13801 ggatgctgca aggtttgagt acaagttgag ggtgccagag gatttatcag tgataggata
    13861 tgatgatatt caaatggctt catggaagag ttataattta acgacgatta gacaaccact
    13921 agatttgttg attgaaaaaa caattaacat acttaagtcc ttattaaatg aagaaaatcc
    13981 agagtcagtc gttgaaatgt taaaacctga attgattgaa agaggttcta cactttaagt
    14041 g
//
LOCUS       DAMKPG010000094        14009 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMKPG010000094 DAMKPG010000000
VERSION     DAMKPG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.099"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            419..1312
                     /locus_tag="PC621_002940"
     CDS             419..1312
                     /locus_tag="PC621_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF4110083.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            1312..2706
                     /gene="gltA"
                     /locus_tag="PC621_002941"
     CDS             1312..2706
                     /gene="gltA"
                     /locus_tag="PC621_002941"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF4110084.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            3072..5291
                     /locus_tag="PC621_002942"
     CDS             3072..5291
                     /locus_tag="PC621_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4110085.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5438..6184
                     /locus_tag="PC621_002943"
     CDS             5438..6184
                     /locus_tag="PC621_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110086.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6184..8064
                     /locus_tag="PC621_002944"
     CDS             6184..8064
                     /locus_tag="PC621_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110087.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            8183..9574
                     /locus_tag="PC621_002945"
     CDS             8183..9574
                     /locus_tag="PC621_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4110088.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9681..10721
                     /locus_tag="PC621_002946"
     CDS             9681..10721
                     /locus_tag="PC621_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF4110089.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11210..11863
                     /locus_tag="PC621_002947"
     CDS             11210..11863
                     /locus_tag="PC621_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF4110090.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            11939..12148
                     /locus_tag="PC621_002948"
     CDS             11939..12148
                     /locus_tag="PC621_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110091.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12288..12854)
                     /locus_tag="PC621_002949"
     CDS             complement(12288..12854)
                     /locus_tag="PC621_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF4110092.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13015..13668
                     /locus_tag="PC621_002950"
     CDS             13015..13668
                     /locus_tag="PC621_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF4110093.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13826..>14009)
                     /locus_tag="PC621_002951"
     CDS             complement(13826..>14009)
                     /locus_tag="PC621_002951"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110094.1"
                     /translation="DFMYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHK
                     FKLYVVNEKYIKGIME"
BASE COUNT         5495 a         1381 c         2365 g         4768 t
ORIGIN      
        1 ggtattgcaa tagattaaag ccttatacac gtttagtttt taaagaaatt agtgttatta
       61 tgaaaaccta agttaattta ttaaataaat aattgaataa aaaaatttta ttatgtattt
      121 ataaaatttt tgttacaaat ttggcataat atagtatact ttaagtgtgt attaggttaa
      181 atctataaca aattagacgt atgatataga cttagtgttt attggcattt acaagaaatt
      241 tgttaatatc aactatttgt agtttgggca gttataatta ctataggggg gatgtccaat
      301 acaaaacaat caaccaagaa ctaatattat attataaaga gttatagttt tgtgtgttta
      361 tgtttttaat taaatttaaa gtgggaaaaa tatatttgtt agaatggaga atagcgagat
      421 gagtaataaa atagttagta agagacaatt aacagatagt atttatttaa tggaaataga
      481 ggcacctaga gttgcaaaat cttctcaacc aggtcaattt ataataatta aaaatgatga
      541 aaagggagag aggattcctc ttacaatagc tgactatgat agagaaaaag gaacagtaac
      601 tattgtattc caaacagttg gtgcttcaac taaaaagtta gcaatgtttg aagaaaacga
      661 ttttgtaatg gattttgtag ggccattagg acaagcaagt gaatttatac atgaagatat
      721 agaagaatta agaaataaaa agatattatt tgtagctggt ggagttggtt ctgctccagt
      781 ttatccacaa gttaaatggt ttaaagagca tggattagat gtagatgtaa taattggtgc
      841 aagaactaaa gaattaatta tattagaaga tgatatgaaa aaagtagcta aaaatgtata
      901 tgtatcaaca gatgatggta catatggatt taatggaaga gttacagacc tattaaaaga
      961 tttagttgat aatcaaggta aaaaatatga ccaagcaata gtaattggac caatgataat
     1021 gatgaaattt atgtgccaat taactaaaga actaaatata cctactatag taagtttaaa
     1081 tacaataatg atagatggaa caggtatgtg tggtggttgt agagttagtg ttggaaatga
     1141 aactaagttt gcatgtgtag atggtcctga atttgatggt catttagttg attttgatca
     1201 agcaatgaga agacagtcta tgtacaagac tcaagaggga agagctattc taaaacttga
     1261 agaaggcgat agtcatcatc atagtaattg tggatgtgga ggtaacaaat aatggatgct
     1321 aaaaaagtaa aagtaccagt tagagaacaa gaacctgctg tgagagcaac aaattttgat
     1381 gaagtatgct tagggtataa taaagaagaa gctatggcag aagctaatag atgtttagct
     1441 tgtaaaaaac caaaatgtgt gggtggatgt ccagtaggaa tcgacatacc agggtttata
     1501 acaaaaatta aagaagatga tatagaaggt gctgctaaag taatagctaa gagcagttca
     1561 ttaccagctg tttgtggtag agtatgccca caagaaagtc aatgtgaagg tgtatgtata
     1621 cttggaataa aatcagatgc tgtatcaata ggtaaattag aaagatttgt agcagattgg
     1681 tcaaaagaaa atgacataaa tttatctgat acagagccta agaaaaatca aaaagtagca
     1741 gtaataggaa gtggtccagc aggtctagct tgtgctggtg atttagctaa aaaaggctat
     1801 gatgtaacaa tctttgaagc aatgcatgag ccaggtggag ttttaactta cggaatacct
     1861 gagtttagac ttccaaaaca agctgttgtt caacctgaaa tagacaatat aagaaaatta
     1921 ggtgtaaaaa tagaaactaa tgtaattgtt ggtaaaacaa ttacagtaga tgaacttata
     1981 gaagatgaag gatttgaagc tatattcata ggttcaggag ctggtcttcc aatgtttatg
     2041 aatataccag gggaaaatgc aaatggagtg ttttctgcaa atgagttttt aactagagta
     2101 aacttaatga aggcttatag agatgactat gatacaccta ttagttctgg taaaaaagtt
     2161 gctgtagtag gtggtggaaa tgttgctatg gacgctgcta gaactgcatt aagattaggt
     2221 tcagaatcat atatagttta tagaagaagt gaaaaagagc ttccagcaag agctgaagaa
     2281 gttcatcatg caaaagaaga aggcattata tttaatacat taacaaatcc taaggaaatt
     2341 ttagtagatg aaaatggata tgttaaaggt atggtttgta taagaatgga attaggagag
     2401 ccagatgatt ctggaagaag aagacctata gaaatagaag gttctgaatt tgtacttgat
     2461 gtagacacag ttataatgtc acttggaaca agcccaaatc cactaatatc ttctacaaca
     2521 aagagtctta atataaataa gaagagatgc ttaataacag atgaaaatgg tcagacttct
     2581 aaagagggtg tatttgcagg tggagatgct gttactggag cagcaacagt aatatctgca
     2641 atgggtgctg gtaaaacagc agcagcatca atagatgaat atttaaaaac aaaagttaat
     2701 gcttaatttg taaattaaag agagggtgtc tcaaaatgaa ctttttagtt catgaggcac
     2761 tttttttgta caaaatcaaa tatgttttaa tcatttcaac aatgaaaatt tcattcctgt
     2821 agaaagtcta acttttaagt cttagtacaa tctctatttt gatgtaggca cttttttaga
     2881 tggtttaata atttatcagt tattattgta atatttgata ctaagtaaca taactttgtc
     2941 taactatgat aaaatagtta tacaataaag gtcttgtaag gagtgaataa tgtgtggcta
     3001 ataaaatcag atttgtcaaa tctaacatta aataatagaa aaataaattg tgtaattaat
     3061 ggtattttaa tatgaaaaaa agagaaaaag tatttgatgg gctagttttt aagcatatat
     3121 taatcttatt aatactatta gttttgattg atttaacaag taccatcata tatgcaagta
     3181 actctatgga aattacttct agaaatatga ttgaggcatc aaaaagagaa cttgaaaact
     3241 atttagaagt aaacatatct ttattaaaag ctcttagtca agatgataga ttttctaatg
     3301 gtgaaacatc actaattgaa aagggaaaat tattaaggcc atatcaaaag gaatataatc
     3361 tttttatgat tggtataaca gatacaaaag gaaatgcttc aagtacatat agggaaaatg
     3421 ttaactcaat aaaagataag cctagttttg agaaagcaat taaaacaaag caagtagtag
     3481 tttcagatat tgaagtcagt aatgtaacag gtgacaaagt atttatcata tatgtaccaa
     3541 ttgtaaaaaa taatgagatg attgggacca tttttgcttc tttttatttt caagatgtaa
     3601 ataatttaat aagtagaagc aattttgatg attctattaa atttctaatg atagataaag
     3661 attatactat tatttctcat ccaaacaaaa aatatgtaaa tgataaaagt aagatgcttg
     3721 atttagaagg gaatattata ggaaccacta aaagtgaaat cttaaaaaat ataaataaaa
     3781 aatgtcaagg tgatttttta tcttgggata attggagatt atataatgta aaatatacga
     3841 acataaagtg gactaattgg actttagtat caaaatgtaa tatatttaag aactttaaaa
     3901 atctaatagt taattttatg ataaagttgt atttttatat agtaatattt atgatattgt
     3961 ggaaattaag taatgctaaa ttaatagaac aacttaaaaa attggcttat tatgattctt
     4021 taagtggaat aaaaaataaa gaaaagttta gaaaagattc tatgtatatt ttaaagaatt
     4081 attatcaaga taatttttac ttagtacaat tggatgtcaa taaatttaaa tatataaatg
     4141 agatgtttgg atatgctgaa gggaataaga tattaattca tatatcacag gttttaaata
     4201 ataatacaaa taaatatgaa atttgtgcta ggatggataa tgaccatttt atattgttga
     4261 ttgcttgtaa tactgaagat gaacttttaa atagattatc taaaataaat aaagaaattt
     4321 gtaacttaaa tactactaac tcatctaagt ataaaattgt tatgtcaagc ggaatatata
     4381 agattactaa aaaggatgat attaaaaaga ttgacctgtt aattgataga gcaaatattg
     4441 cagcaaaaag taaaaaggaa aaatatgaac attcatattc gttttttaat gaagatacaa
     4501 gaaatcgatt gtacaaggaa aaacgattgg aagataatat gaataaagct ttagaaaaag
     4561 gtgaatttat agtatattat caacctaagt atagtttaga tgatgtaaat gaaattgaag
     4621 gagcagaagc cttgataaga tggaatagtc cagaatttgg gtttatttca cctatagatt
     4681 ttgtaccttt atttgaaaag aatggtttta ttgtaaatat tgatatgttc gtgtttgaag
     4741 aagtatgcaa aacattaaac aagtggataa ataaagggta tactcctgta ccaatctctg
     4801 taaatatgtc tagagttcat ttatataggg ataattttat agaaaatata actgatttga
     4861 taagtaaata caatatatct cctgagttta ttgaacttga actaacagaa agtgttgtgt
     4921 ttgataattt aaatatattg attgatataa tgaaaaagat taaagagata ggattcctta
     4981 tatcaatgga tgattttggt tcgggatatt catctttaaa tctgttaaaa gacctttctt
     5041 ttgatatttt aaaattagat agaggatttt taatcgaaac aacagataca aaacgtggaa
     5101 aaattattat ttctaagatt gtggaaatgg caaaagcgat tgatattaaa gtaatctgtg
     5161 agggcgtaga aacttatgaa caagtagagt tcttgaaaga gataggttgt gataaggttc
     5221 aaggatactt atttgctaaa cctatggtat tagatgaatt tgaaaaacac ttaaacttta
     5281 aatttgatta atcattaata attattagac tataatatac cctaacaact tacttacaaa
     5341 tatgtaagtt aattgttagg gtatttttat ggttaaagta gtatattcac gatattatgg
     5401 tattgtagta aaaataaaaa attatggaga atataaaatg aacatattga gtattagaaa
     5461 tatatcaaaa acatattgcg gaaatattcc atttaaagca ttagataagg ttagtctaaa
     5521 tatagaaaag ggagaatttg tgtctgttat gggaccatct ggaagtggta aatctactct
     5581 tttaaatata atatctacag ttgatagaca gagtgaaggt gaggttgtat tagatggata
     5641 tgatgtaagt aaattgaaag gagaaaaact tgctgaattt agaaggaaac aacttggttt
     5701 tgtatttcag gattttaatt tgattgacac tttaactgtt ggcgaaaata ttatgttgcc
     5761 acttacacta gagggggcaa gtatcaaaga tatgaatata caaactaaaa gtatatctaa
     5821 atttttagga atagataaaa ttttagatag aaaaacctat gaaatttcag gaggtcaagc
     5881 tcaaaggtgt gctattgcta gagctattat aaataaacct accatacttc ttgcagatga
     5941 gccaactgga aatcttgatt caaaatctac tgatgatgtt ttgaaattat ttactaggat
     6001 aaacaaagaa caaaatgtaa caacacttat ggtaactcat gaagtatata gtgcaagtta
     6061 ttcagataga gtaattttta taaaagatgg ttgtatatat acagaaataa aaaaatctga
     6121 aagcagtaat tctttctatt ctgatatact agctgttttg tctcaaatag gaggtgtaag
     6181 atagtggatt ttagaaagtt tgcttataat aatatagtta gaaatttcag ggcatattta
     6241 gcatattttt taagttctgt tttttctata atgatatttt ttgtttttgc agtgtctatg
     6301 ttccatccga ttataacaaa atctggaatg cagagtggtt ctactgcatt tatggctctt
     6361 atgacttcag aattaacaat attaggtttt agtctacttt ttatattgta ctctttagga
     6421 aatttcttga aatatagatt gaaagatttt ggagtattga tgataattgg aatgagcaac
     6481 agacaactga aaaaattgat attaattgaa aatttgataa taggattttt agcagtgatg
     6541 ttaggaatat tacttggttt atcaatttca aaattgtttt tattgtattt aagcaaactg
     6601 ttttatatga atttaactga aatttatttt ccagtaaaag caatactatt gactattgta
     6661 tcatttatga ttttattttt gataacagga ccaatgagct taaaattatt gagaaaaaag
     6721 agtattttag agcttttagt aggtacaaaa aagcctaaaa aagagataaa atcttcaagg
     6781 ctatttggaa taattggtgt aatatgtctt atattaggat atgctatgat actatttggc
     6841 aataagttaa aaataaatgg aggtatgtat acaacagcac tattaattac tttggggata
     6901 tttttatttt tttctcaatt tactattttg atattaaagt atttaaaaaa taaagaaaaa
     6961 ttttataaga acaaagtaaa tatgttactt ataagtaatt tgatatataa attaaaggat
     7021 aatacacgat tgttattttt aataacaata cttttatcgg ctactttagt agcatttaca
     7081 acaacttcaa cacttgtaag ttctcaagga gaagcttcta aaaataattt cccaatggtt
     7141 tactcatatt tttcagagca taataatgaa aaagaatatg aagaactaca acaaattaga
     7201 gaagcaatca aaggatatga ttataaggag ttaagttttc ctgtacttaa atataatagg
     7261 gaagtcttac taagtgtaaa gaattataac aaattatcaa aggaattggg attagataaa
     7321 attagttcaa agttagaaaa atatgaaggg attataattc ctagtagcaa ttcacaagaa
     7381 catataaatt ctttaaaaca attaaaaaat tatagtataa aaaatttaaa tatagaagta
     7441 aaaaaatcaa aaaataaaat aatttttcct acaggactgt ttagtaaagt tgtagttata
     7501 agtgatgact tatacgatga agtaaaaggt gaattttcaa aaattaattt tcatggattt
     7561 gattataaaa actggcaaag cagtgcaaag ataactgaaa atttaaaaga aaaacatttt
     7621 aatttggata gagaatataa caggtcatat tttttaacat taccagatat gtatttagta
     7681 gagttacaac aaagtaaaat tcttcaattt atgggtgttt ttataggagt gatattattt
     7741 atagcagttt ttagtttttt atacttcaga ttatatacag acgagataga agataatgta
     7801 aagtacaaaa aactatcaaa aataggtcta tcttttaaaa atatgaataa gattgttagt
     7861 atagagatag gtacattgtt ttttataccg tattttatag caattataaa tagcataatt
     7921 tctttactgt ttttaaatca agtattaaat aatagtttta ggttagaaaa tttgggtata
     7981 ttactactga tatcatctat atattcactt tatttttact tattaaaaaa aatttatact
     8041 agaaaagttt ggttttttga ttaaagtggt atataataga tatataaatt aatatttaaa
     8101 aagtagttaa tattagggga gggcatttgc acctcccttg taatatgtgg aaaattgaat
     8161 attagaagaa ggaggagact tgatggaaaa tcaacaatta ttaggaacag aacgaattag
     8221 taaattacta ctaaaatatt ctataccagc tataataggt atgttggtaa atagtttata
     8281 taatgtagta gatagaattt ttattggaaa tataccaggg gttggaccac ttgcaataac
     8341 aggtcttggt gtaacaatgc caataatgac aataatactt gcatttggga tgttgatagg
     8401 tataggaact actactacca tatcaataaa acttgggcaa ggtaaagtag aagaggcaag
     8461 aaaacttata ggaaatgcaa tgacgttatc tgtaataaca ggaattatca taatgatact
     8521 tggaatatta tttgcaaata aaatacttac attgtttgga gcaagtgaaa atactttaat
     8581 atatgcaaaa tcatatataa atattatatt attaggtaca gtagtaaatc ttctttcctt
     8641 ttctttaaac cactcaataa gagctgatgg tagtcctaaa atttcagcag gaataatgat
     8701 agtaggatgt ttaactaata tagttttaga ctggatatta atatttggat tcaatttggg
     8761 aatacaagga gctgcaattg ctacagtaac ttctcaggct cttacagcta ttttaactat
     8821 agggtattat ataagtggaa aatcaaactt acgattcagt aaatctaatc taaaattaga
     8881 taaaaagcta ataaaagcag tgtttgcaat aggtatgtcg ccatttgcga tgcaacttgc
     8941 agcaagttta gttcaagtaa tatcaaatat tgcacttaaa actcatggtg gagatttagc
     9001 tataggggct atggctacta tttcttccat tgcaatggta tttttgatgc ctatctttgg
     9061 aataaatcaa ggagcgcaac ctattatagg tttcaattat ggagctgaaa aatatgatag
     9121 ggttaaaaaa gcatatttag gttcattagt agtagctact ataattttat gtatgggtat
     9181 ggtagtagtt atgcttttcc cagaagctat tataggtata tttaataaag accctgaact
     9241 tatgaatata tctgttaatg gattaagaat atacttactt atgttgccaa ttgtaggctt
     9301 atcagttaca ggaacaaact ttatacaatc tataggaaaa gcaaaaatgg ctatgttatt
     9361 gagcctttta agacaagtaa ttttattgat accagcagta ttaattcttc ctacattctt
     9421 aggtttacag ggtgtatgga cagctcaacc agtgtctgat tttatagcta cagtaattac
     9481 aggtatagtt gtatttagag aattaaaaag atatactcct aaaactgaaa aattaaatga
     9541 aaatgaaaga ctaaatgaga taactactga gtaaaaatat aaattcagat aattgagcaa
     9601 gaaattgcaa aaaatacgat tatatgataa aataaaaccg ctgtcaagga taagagaaat
     9661 tctaattatg gagtgatttt atggaattta caaagaaaag caaattaaaa aatatgtatg
     9721 taaacatgaa tgtagctaga atgttgtcta agataaatga gtataagggt aggcaactgc
     9781 tttacaaaaa acaaccaaaa gaaattttag aaaacctaga aaaaaaatct ttagtagatt
     9841 gttctgaatc tacatacatt ggaaatcaaa gagatagtag taattttaat ctagaaaagt
     9901 tgataagtaa tgaagttaca cctagaagta gagaggaatt gagtattgtt gaatacagag
     9961 atgtggtaaa aactattaac agtgcttatg agagtattcc catcagttca caaacgattc
    10021 ttgagcttca tgggtactta tataaatttt catctactag aggaggaagt tataagtcag
    10081 ataatgattt tattgaacac aatttaaaac ctagcgaatt tataagtatt gattcaagtg
    10141 tcaataaagt tgaaaaagct gtagaagaaa tatgtgaagc ttataataca ttgatagagg
    10201 aagatgaaat tgatatatta atattaattt ctgcatttgt gttagatttc atattgatac
    10261 atccattcaa agaaggtaat ataaaaatgg cgagagtact tatcctattg ttactaaata
    10321 aaaatgggta tgaagttgga agatatataa gtttaggaaa gatatttgat gatagttcat
    10381 atgaatatta tagtaattta aattatttga aagcctcaat aggtagtgaa aaagcagata
    10441 tgaatgcatg gattgagtat tttttagaga cgatattaac tgcatatgag aagctggatg
    10501 atagtttaaa tatatctgat aagaaaagac aaacaaaaac tagtagaata gaaaaaataa
    10561 taaattctac ccttggatat tttacaaaag aagatataag agatttatgt cctgacattc
    10621 cagaacctac tataaataga gtttttaata atctaagaaa acaagataag atagaagttg
    10681 ttgcaagagg aagaagtgca aaatggaaga aaaaatatta aaaaatgttt aaataactaa
    10741 aatggtatag tgttttacac tataccattt ttaatgtcat tagcatgagc aataatttaa
    10801 caacttaagc aatatcaatt ttatttattg attatttttg aactgaaaaa tggggttata
    10861 tggtatacta caataataat taattttgga tgagaatata taacgttaca gaatatctta
    10921 ggagtatatg tataaaaaat ataataaaag tgtgaggttt agtaattaac aaattatgtg
    10981 gcttcacact tttttataaa ataaaaatac ttagcagaag aatattttag ttgatatgtt
    11041 tataagatag tacttgtttg ggtaaaagta taaatagtaa aaaagtagaa gtaatgtgta
    11101 taaaaacata agttacattc tgtaataata gttattttat aattcaacaa aagaatagaa
    11161 tgggtatatt tgtgaaataa taatataaaa taatcttagg agtgatctta tgaagaaaaa
    11221 attattatat ataaatgtaa attcaaaacc tgaagactta tcttcaagta aaactgtagc
    11281 aagaaaattt ataaataaat ttatggaaaa aaacaaagat tttgaggttg aagagataga
    11341 cctttataaa gaacatatac cgagacttga gtatcagtac tttgaaaaaa gaaatagcat
    11401 agttagtgaa gaaaatgcta aaaatttaga cgataaggat agaaaagagc ttacaaagat
    11461 tagaaatctt tgtgaccaat ttgtgagtgc aagtgtttat gtaatagcag cacctatgtg
    11521 gagtttatct tttccagcac cacttaaaga gtatatagat tgtataattc aagatggaaa
    11581 aactatatct tttgaaggca atgataaacc acaaggaatt ttaaatgata ttgatagaag
    11641 tatggtatat atacaatctt ctggtggaca cataccatgg gtcttgaagc cagttatgaa
    11701 taaagggtta aattatgtgg aaagtattat gaaatttata ggaattaaaa aatttgatga
    11761 attgttagta gatggaactg gaactagtga agaagaaaga caagctgcaa tagaaaaagc
    11821 atctgaaaaa atacatggta taatagatgg tatgaaattt taattataat attttattaa
    11881 aaatataaag gaaaaacctc ttttaatgta gaaacttata ttgaaggagg cgagaatcat
    11941 gcgagaagaa aaaagtaatg aaaagtatga ttgttattgg tgtaatcaag aaaataattt
    12001 ctgtgtagaa ataaaagata atatagtaat gatagatgat ggcactggta cgttaaaaca
    12061 agccgttttc atagggtata aacaaatcca aattaattta aattgttcac attgtcaaaa
    12121 cttaaataga ataaaattaa atttgtagaa ttttaaaatg gaaattattg aaggaattaa
    12181 aaatagaaag tgttaatatt tagactgaaa attatatttt atacaataaa agtttattta
    12241 aataacattg tattttaata tttattttca gtcttacttt attaaaatta ttttttggtt
    12301 atgtctggaa aatatctgtc tactaatttt tctgaaatat tttttaattg gtcagccatt
    12361 gaagtcagac ttaataaggc tatcaataaa tcatctttat tttctggatt tatactaata
    12421 ttttcaaaat tagactctat aaagtccggt atattctttc tttgattttc ttttattaac
    12481 atgaattttt cgtaacatga aactaaatct ttttcactga aattatcctt ttcatacaca
    12541 ccatgtaaga ttcttttaat ttcttgtatt gtaagtgtat ttttcataga ataaataatt
    12601 atcatctgta aaatttgttc tttagtatac ttttttccct taactggttt gattaatccc
    12661 tctttgctgt aattatttat catggtttta gtaagaattt tatcactttc aaatctttta
    12721 ttaggagaaa gtttattgtc aattagagaa ataatttgat ccatatataa gtcaatagag
    12781 ggaatatcgt ttgaagttat atctgcatta tttagtgttt ctagtattat agtattaagt
    12841 tcattattca tcatatgtta cctccataat atatataata cactaaatta gaaagtaaaa
    12901 caatattaag acttgtaaaa aaaacataaa gtggtataat taaatatata atatagtaat
    12961 gaaaactata tttatgaatc tgatttttaa aatacataat tgtaggagga cgaaatgtat
    13021 caattttttt taaagggtag agacccaata agcagtttaa ctcattttat aggtgcttgt
    13081 ttatctcttt tagcaactat aattttagtt tttcaatctg ttacattaca ggaaacttct
    13141 ctcttgatga tagtatctgt atcggtattt ggactttcac taattgcttt atatagtgca
    13201 agttcgtatt atcatttttt aaaggggaca ccagaacaag aattgttttt tagaaaagta
    13261 gaccatgcaa tgatttatgt attgattgct ggctcttata caccaatatg tctgaatttt
    13321 atggaaaaaa aagaaggcat aatatttgtt acagcaatat ggatagttgc atttattggc
    13381 ataataataa aaatattttg gatggatgca cctagatggt tatctaccag tatatattta
    13441 cttatggggt gggcaattgt ttttgatata aatgcattca attcaattcc taaggattgc
    13501 cttagactat taattatgga aggtgtatct tactctatag gtgcaattat ttatataatt
    13561 aaaaagccta atataagtcc tgagtttgga tttcatgaaa tatttcatat ttttattatg
    13621 ataggttctt tatttcactt tttagcagtt ttattgtatg ttttatagtt ttaaaagatt
    13681 tgattaattg atactaaaat tctacaatga ataaaatgga actgccttat atagattata
    13741 tcttcgacaa taagacagtt ccttattata aatattaaat tctacttata ataaaatttt
    13801 gataaaaata gtccattcaa aatgcctatt ccataattcc tttaatgtac ttttcattta
    13861 cgacatatag tttgaattta tgtactataa ttatatcctc taaattctct gaaacactaa
    13921 caattctgac acatttgttt ctaatttcta caggtaaatc taaatcattt gttaaattca
    13981 catagtcatc atatttatac ataaaatca
//
LOCUS       DAMKPG010000095        13382 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMKPG010000095 DAMKPG010000000
VERSION     DAMKPG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.100"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..344
                     /locus_tag="PC621_002952"
     CDS             <1..344
                     /locus_tag="PC621_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110095.1"
                     /translation="IKDNDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKAN
                     WYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILK
                     EGLRLITIQNK"
     gene            570..2882
                     /locus_tag="PC621_002953"
     CDS             570..2882
                     /locus_tag="PC621_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF4110096.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2970..3305
                     /gene="spoIIAA"
                     /locus_tag="PC621_002954"
     CDS             2970..3305
                     /gene="spoIIAA"
                     /locus_tag="PC621_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF4110097.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            3319..3747
                     /locus_tag="PC621_002955"
     CDS             3319..3747
                     /locus_tag="PC621_002955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF4110098.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3770..4534
                     /gene="sigF"
                     /locus_tag="PC621_002956"
     CDS             3770..4534
                     /gene="sigF"
                     /locus_tag="PC621_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF4110099.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4814..5251
                     /gene="spoVAC"
                     /locus_tag="PC621_002957"
     CDS             4814..5251
                     /gene="spoVAC"
                     /locus_tag="PC621_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF4110100.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5405..6418
                     /gene="spoVAD"
                     /locus_tag="PC621_002958"
     CDS             5405..6418
                     /gene="spoVAD"
                     /locus_tag="PC621_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF4110101.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6432..6794
                     /gene="spoVAE"
                     /locus_tag="PC621_002959"
     CDS             6432..6794
                     /gene="spoVAE"
                     /locus_tag="PC621_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF4110102.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6977..7993
                     /locus_tag="PC621_002960"
     CDS             6977..7993
                     /locus_tag="PC621_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF4110103.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            8368..9210
                     /locus_tag="PC621_002961"
     CDS             8368..9210
                     /locus_tag="PC621_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF4110104.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            9225..9689
                     /locus_tag="PC621_002962"
     CDS             9225..9689
                     /locus_tag="PC621_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110105.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            9676..10848
                     /locus_tag="PC621_002963"
     CDS             9676..10848
                     /locus_tag="PC621_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4110106.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            10892..11827
                     /locus_tag="PC621_002964"
     CDS             10892..11827
                     /locus_tag="PC621_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF4110107.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(12626..13024)
                     /locus_tag="PC621_002965"
     CDS             complement(12626..13024)
                     /locus_tag="PC621_002965"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF4110108.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
BASE COUNT         5117 a         1583 c         2492 g         4190 t
ORIGIN      
        1 taataaaaga taacgatatt atttgcatag aagatttaca agtaaagaat atgattagaa
       61 atcgtaaact atctcgctta atttctgatg tgtcatggtc agaatttatt cgtcaattag
      121 aatacaaggc taattggtat ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt
      181 cacaaatatg caataaatgt gggtataaaa acgaggaagt taaggattta aatataagag
      241 aatggatttg tcctagttgt aatgaaactc acgatagaga tataaatgca agtataaata
      301 tattaaaaga aggattaaga ttaataacaa ttcaaaataa ataacttatg taagaaccgt
      361 aggaactacg gggatagcct gtggagaatt agtaagacat attttagtat gcaaaattct
      421 gtgaagcagg aacactgtga ctttagtcat gggaggttca gaaaatatag ctatgtttta
      481 tatactgtag ttttgaacat agaaagttta ataaattata gtatatatta atttttaata
      541 aggtaagaaa gtagaaagaa ggatatttta tggatataat aaaaaaatta agtcatggat
      601 gtacattaga ttgtcatgat tgttgtaaat tcaatgttta tactaaagaa aataatgtag
      661 taaaaattga aggagataaa aatcatcctt atacaaaagg ttttatatgc aaaaaaggga
      721 tggctcattt agatagatta aatcataaag atagaataaa gactcctatg cttaaagttg
      781 atggagtatg gaaagagatt tcttttgata aggctataga aataatggca gaaaagctta
      841 catactataa agaaaagtat acatcaaagt ctgtcatgca ttatgaccaa tatggaagtg
      901 ggtctgtgct aaaatacata ggagatatat tttttaattt ttatggaggt gttagcagac
      961 ataagggtgg tccctgttgg agtgcaggga tgcatgctca aaaatatgat tttggagtag
     1021 ctaagagtca tgctatagaa gatatgttaa atagtaaaag tatatttgtg tggggaaaaa
     1081 atccagcata tactacaatt catactatgc aaataattaa aaaagctaaa gaaaaaggga
     1141 taaagatagt agtgattgac cccatatata caaaaacagc tcaaatagca gataagtatg
     1201 tacaggtaaa tcctggaaca gatggagctt tagctatagc tatggcaaaa attatagtag
     1261 aagataaatt atatgatgaa gaatatataa actcgtatgt tattggtttt gaagaatata
     1321 aaaaatattt atcttcattg gaattaagtt ttttaattga tgaatgtggt gttaaagaga
     1381 atgatataag agagttagtg gatttatata ctaacaaata ttcaagtata aatgttggat
     1441 atgggcttca aaaatataaa aatggtggaa acacaataag agcgatagat gctttaggtg
     1501 ctataactgg tcaaatagga tttagtggtg gaggggtaaa ctatgctaat aaagtttatc
     1561 caagtgttat aaattctgac ccttacaata gccaatcata tggtgaagat agggagtttt
     1621 atgtaagtaa tatcagtaaa tttatagaag aatctttaaa aaatacatca aataaagtaa
     1681 attatgcatc agatgaactt gatacaacat caaataaagt aaattacata tcagatgaac
     1741 ttgatacaac atcaaataaa gtaaattatg tatcagatga acttgataca acatcaaata
     1801 aagtaaacta tgtatcagat gaacttggta tggcattaca taaagtaaat tgtgtatcag
     1861 atgaacttga tatgacatca aataaagtaa attatgtatt agatgaactt gatataacat
     1921 caaataaaac agattatata tcaaatgaat tgtataattt atctaataag tcaatcaaag
     1981 ataatattcc tataaagatg gctgtgataa ctaaaagtaa tatgctgaat caactgccag
     2041 atttagtaga attggagagg gtgttttcaa aaattaaatt taaagtatgt tttgatatgt
     2101 ttatgactga tacagctact ttatgtgaca tgtttatacc gtgtacaaat acactagaga
     2161 gtgaagatat aatttatagc tcaatgacca atccatatat aacctataat gaaagagctg
     2221 taaagccagc acataaattg atggatgaat attatttctt tatggagcta gctaaaaaaa
     2281 tgggattaaa tgattatcct tttgtagaaa agagaactta tttagaaaaa gtgattgaac
     2341 cacttaagcg ttttgacaaa aatctggata tagaaaaact taaaaacaat tactttacta
     2401 tacataacct tgttgcttgg gaagataaga agtttgagac tccatctgga aaatacgagc
     2461 tttattcaga aagtataaaa aacttgggaa taagtccaac acctgtatat ataagcaata
     2521 aatataaaga aatagaagat aaaaatattt cttttagatt attaactaat catcatgcag
     2581 atactttatt tagtcagcat tttatggaca aaaaatctat agctcaagca tatataaatc
     2641 agagaatggc taaaaaagta ggtattgagg acaaagatat tgtgatattg aggtcaaaaa
     2701 aggcaaaaat aaatgtacaa ataaatatag atgatggagt aggtaattat atagtaaaaa
     2761 tgtatgttgg ttggtggaag aaacatggaa atcctaatag tttaacagac actggtatat
     2821 cagattttgg aggtcaggta acatataatg aaagtatggt agaaataata cgacaaaact
     2881 agttaaattt cattgaagga ataaaaatat aattatagaa ttgattaaaa gttactattt
     2941 ttacaaaact attgaaggag ggatttttga tggtaaatta ttccttagaa cataagaatc
     3001 tatacataga atttatgtgc tcagaactag accaccatgt agccaatgaa ataagggaag
     3061 aaatagataa cttattaagt gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata
     3121 ttaattttat ggattcttca ggtataggcg tcatcatagg tagatataaa aagatttcta
     3181 atgaaggggg acgagtttca gtaattaata taagttcacg agttaagaag atttttgatt
     3241 tatctgggtt aaataaaatt attggcattt atgatacgta tgaagaagct ctaagttctt
     3301 tgtagggagg agtatgtgat gaataatatt atggaagtta aattttcggc aatatctgaa
     3361 aatgaaagtt tagcaagagt tatagtagca tcctttgcag ctaagttaga cccaacagta
     3421 gatgagttag tagatataaa aactgctgtc tctgaggctg ttacgaatgc tataatacat
     3481 ggatatgaag aagacagttc taagtttgtt ttcttaagat gtgagataga aggaaatact
     3541 ataaagatta tagttgaaga tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa
     3601 ccactttata catcaaaacc agaacttgat agatcaggaa tgggattcac agtaatgaaa
     3661 agctttatgg acgatgtaga ggtaagttct gtaaaagata atgggactag aattgaaatg
     3721 actaaaaaaa taaatgtgcc taaataaagt ccaaaagtga ggttttatta tggaagtaac
     3781 tgttgccaga gaagaaaaaa aacctcttct tagtcatgaa gagactctag aacttattga
     3841 aaaagttcaa aatggagatg aagaggcaaa agaaatatta atatcaagca atttaggtct
     3901 agttaggagc gttgtatcta gatttttaaa tataggatat gatagagaag atttatttca
     3961 gttaggttct ataggcttaa taaaatcaat atataaattt gacccaaaat ttaatgtaaa
     4021 attctctact tatgcagtac caatgatttt aggtgaaata aagagatact taagagatga
     4081 tggtatgata aaagtatcaa ggtcgttaaa gcaaatagct gtaaaggcaa agatggaaag
     4141 tgaagcttta actaaaaaat taggtagaga gccaagtata gaagaattag ctaaagcaat
     4201 agatgtagaa aaagaagatt tagtaatggc tatggaagct aatttcaatg ttgagtattt
     4261 gcaaggagtt attcatgaag aggaaggttc tccaatatgc cttattgata aaataagcat
     4321 gaaaggagaa agtgaagaag aaaaagtagt ggataacata ttgttaaaag acatacttgg
     4381 aagactggat aagagagagc gtcaaataat agttctcaga tactttgaag acatgactca
     4441 aagtgaaata ggagagatgc tcaatatatc acaagttcaa gtatctagga tagagaagaa
     4501 agtattgtct aagttaaaag aatatatatc gtgataatac ttatgatatg tagaataaca
     4561 aaatataata aatatattac taaaataaaa tgtaataact ttttatgtta agtttttaaa
     4621 ttattaaaag atactaaaat aataaaaagc attaaaaaag taaaaatata aaaacaaggt
     4681 gtctttatgg aaaaatagat tttttcttta ttaggcatct tttttatttt ttgaccaaac
     4741 tttagatagt caaaataaat aataggttta tttttttaaa attaacaaat aatactttag
     4801 agaggtgttt agtatggata aaaattataa aaaatatgta gaccaaataa gcccaaaacc
     4861 tacatattta aaaaactata cacttgcatt tattgtaggt ggaattattt gtatgatagg
     4921 gcaagcaata aatgatttgt atatgaaagt tggaggtctt gataaactag gtgctagttc
     4981 tgcaacatca ataacattaa tatttatagg ggcattttta actggattag gagtttacga
     5041 cctaatagga aaaagagctg gagctggttc tataattcca ataacaggtt ttgctaactc
     5101 aatagtatct ccagcaatgg agtataaaag agaaggctat gtattaggtg taggtgcaaa
     5161 cttatttaaa attgcaggac cagttttagt ttatggtata ggttcttcta ttttatgtgg
     5221 aattatttat tatattttta agatgtttta gatgattttt atttaattta aattttatta
     5281 attatgaaga aggtataaaa tctgaaaata gctactaaag taactaattg aaaaataatt
     5341 gtgcagattt aattaatatt aaaaatttga atagtaaaat gcaaaattaa aaaggtgtga
     5401 aaatatgaaa aataaaagaa ttggaaaaag aacagtcaag ttagaaaata aaccaaccat
     5461 aatatcaact ggaactatag tgggtcctaa ggaaggtgaa ggaccactaa aggactattt
     5521 tgatatgatt atgactgacg atttatatgg tgaaaagact tgggaattag ctgaaagtaa
     5581 aatggtagag actgcatccc aacaagctat ccaaaaggca ggtaaaaaat tgtcagatgt
     5641 aaattatatg ctaggtggcg atttaataaa tcaaatagtg ccagcatcat ttgcagcaag
     5701 agaactggct attccatttt taggtatata tggagcttgt tctacaatgg cagaaggtct
     5761 ttgtataggt tctatgctta tagatggtgg ttttgctgat ttagtactgt caggtacatc
     5821 aagtcactat tgtacagcag aaagacaatt tagattccca cttgaacttg gaaatcaaaa
     5881 accaatgaca gctcagtgga cagttacagg agcaggaagt gtattattag ctcctaatgg
     5941 agacggtcca aaagtaaagt atgttactgt tggtaaagta atagatgagg gtattgatga
     6001 tggtaataat atgggaccag ctatggctcc agcagcaata gatactatct actcttactt
     6061 tgaagatact aatgatgacc caaatagctt tgatataatt gctactggag atttaggtac
     6121 tcttggaaaa cagatagcag aagactttct aaaagaaaaa ggtgtagata tatcaaaagt
     6181 ttatactgac tgtggtatag agatgtttaa ccttaaagaa caagatgtcc actgtggtgg
     6241 aagtggatgt gggtgttcag ctacagtgtt tgcaggatat atatatgata agttgagaaa
     6301 aaaagaattt aacaaagtaa tgcttgtatc aacaggagca ctactttcac cgacaagtac
     6361 actacaaaag cagacaatac catgtgttgc acatgcagta gtaatagtaa atgagtagga
     6421 agtgatataa tatgttaatg gactatgtaa gagtatttat tgtaggtgga attatatgtt
     6481 taatagccca agtaatgatg gattatttca aaatgcaaac tccatatgtt atggtaacgt
     6541 atgtaacaac tggtgtagtt ttaacttttc taggacttta tgagccatta gttaagtttg
     6601 cagcagcagg tgcaacagtt cctataatag gatttggata ttcacttgct acaggagtaa
     6661 taaaatcaat tcaaagtgat ggttttttag gtattttttt aggaggtaca actgctacag
     6721 ctggtggaat agcagcagct atattctttg gatatattat gtcagtagta tttactccaa
     6781 aagcaaaacc ataaatcttt attaaaagaa taataagttt ttgaaataat aactttttat
     6841 aaagcaggtg gatatctttt agattccacc tattttttat ataaagaagc gtttacataa
     6901 ttgaagttga caattattta caacatagtg tagtattata ttatcaaaat aaaatgaagt
     6961 gaagtagggg ataaagatgg taactattaa agatattgca aggaatctag gtatcagtta
     7021 ttcaactgtt tctaggtgct taaacaataa tcctaatgta tctgaaaaaa caaaaaagaa
     7081 agtagtagaa gaggcaaata gactagggtt ccattttaat gtaaatgcaa gaaatttagc
     7141 aaaaaaagaa actaacagga taggggtaat cttttcaaat aattttaatc atcaagatac
     7201 tagaaagttt tttagtgata taatggatag ttctataaat tctattgaga ctaataaata
     7261 cgattttata atacagccta ataataacat tagtggtgat agtaatgtat ataaaatggt
     7321 aaatggacaa atggttgatg gattagtaat tgtgtctcaa tcaataaaaa aagaagagta
     7381 tgatttttta aaagacaata attttccaca tgtatttata tattttaaac catcttttgt
     7441 aggagaagta gacaatttct tttgggacga taacgtgtat ggaggttata tagctaccaa
     7501 gcatttaatt gaccatggtc ataaagacat aataacagta acttcagatg ataggagttc
     7561 aaaaatgcat gaagatagaa ctaagggata tttaaatgct atggaagagg caaatttaaa
     7621 gaccgaagtc attaaatcta aaatggattt tgaatctcaa gttgaatttt tagaaaaata
     7681 tattgataaa ataaaaaaag cttcagctat atttgtacaa caagatgtac ctgcgatttc
     7741 tataatacaa gagttaaaaa ctacttatgg tataaatgta ccagaagata tttccataat
     7801 agggtataat aatattgaat taatatcata ttttaggtcg catttatcaa caatagatga
     7861 cccaagagag caagttataa aaaatggagt agatagttta gtaaatataa taaataaaaa
     7921 gagtacagaa cacattccaa gaaaattata tccaaggtta ataatacgaa atagcgttaa
     7981 aagaataaag tagtaagctt ttaagtatca aaagtttatt aaaaatatta tagattatgt
     8041 gaagtttttt aacaaacaat ataaataaaa attaaaagga gtaggtgtat ataagtacat
     8101 tgtactcctt tatttattga gatagttttt ataatatgaa tgaataattt tattcaatta
     8161 tttgaaaatc cgttatttta acaatataaa ttaataaatt atttgtattt tatatcatga
     8221 atctataata tttttttgat tcataactaa aagtatataa aaatgatgaa taaatatata
     8281 ttatacatta tcaatgatag gttatataat aaaatcacta acaattatat tataagaaaa
     8341 ttataaaaat tacaaggagg tatagaaatg aaagaaaaag cagggattag aaatgccata
     8401 atagcagcct ttataatcac aatattggca gaattaattg gacctatttc atttaaagtt
     8461 atgggaataa atgtaacttt acttagtata ctatgggcta tattcatagg aatggctgtt
     8521 tctcctcatt tattagggag agttatacca gccttaaaaa agtttatagg ggataatgaa
     8581 ataaatgtat ctccatattt attatctttg acactatatc cattaggaat aatgtttgga
     8641 attaatgcag ggcctaaagt tggtatatta cttcaagcag gaccagcact tttattacaa
     8701 gaatttggtc atatgggtac aatgttgata gcattacctg ttggagtagc attaggtctt
     8761 ggaagaagtg cattaggtgg tactttttca ttatgtcgtg acacagcact aggcattatt
     8821 ggagataagt atggattaaa ctcaccagaa ggtataggaa cactaggaac ttacataagt
     8881 ggtagtatat ttggaacatt attattttca tttttagcac cagtgggtgt acttttagga
     8941 tttcatccat atgctttagc aatggcatca ggaatgggta gtggttcaat gatgggagct
     9001 gcaacagcat cattaatgaa tacggttcca caaatgtcag agcagatttt agcatattca
     9061 gctactagtg gattgattac agctgttact ggaatatata tagaattatt cttatcttta
     9121 ccagtagcca actactatta tagcaaagta gctccagtta ttgaaagatt tagaggaaga
     9181 aaaacaagaa gaacaagtaa taaattgtaa aggaggatat atatatggaa aaaactttta
     9241 agactattat ggagcagata aaaatagtat tgtttatata tattattact tgctttggac
     9301 aaatgatagc acttaagact caggctcaag atttgttgat tggaagtata tctggatttt
     9361 tgttggtgat aattgctatt ataataaaga attttgttaa aaaacctaat ttaccaggtt
     9421 ttgcatgggc tacattagtt gcattcgttt tatcgcttcc aatatctcca gtgggggata
     9481 tcatagcaac aaatgtaggg aaaatcaatt ttatgtctac agtaacacca ttattagctt
     9541 ttgcaggaat ctctgtaggt aaccaattag atatattaaa gactttgagt tggaagttag
     9601 tagttatatc atttatagtt atgacatcta cttactttgg ttctgctttt atatctcaaa
     9661 cagttttaag attaaatgga atgctttaag gaggtagtaa atatgaatga attaaaagca
     9721 aaaattaaag aggaaataaa gttattatct gaagagaaaa agacatcatt tgaaaaagtt
     9781 tctgactatt tattttcaaa tccagaatta gcatttgagg agtataagtc acaaaaagca
     9841 ttatgtgatt tgttagaaga aaatgaattt aatgtaacta aaggtgtagg aggattagaa
     9901 acatcttttg aagctgtgta ttcaaatgga actaatggga aaacagttgc ttttctagct
     9961 gaatatgatg cacttccagg aatgggacat gcatgtgggc acaatatcat agggacaagt
    10021 tctgttggtg caggtatcat attaaaagaa atcatgaaaa aacataatat agaagggact
    10081 gtaaaggttt ttggaacacc tgcagaagaa agagtaggtg gaaaaataac tatgattaaa
    10141 gaaggtgtat ttaataatgt agatgctgca ttaatcttac atccaagtga tgcttcaatg
    10201 cctgatgata tatcatttgc tcaagttaat ttaaaatttg attttactgg taaggcatca
    10261 catgctgctg cttttccttg ggaagggaaa agtgccttaa gtggagtaat agcactattt
    10321 aacagtgtaa atagcatgag attacatctt aaagattatg caagagtaca tggaattata
    10381 actgatggag gtagcataca taatataatt cctgaaaaat ctacagctat atttaatgta
    10441 agagccttaa gcatagaata tttaaatgag atttgtgaaa tgcttaaaaa ttgtgctaaa
    10501 ggagctgcaa tttctactgg aactaatgta gaaatagttc aattagatga aatatacaaa
    10561 gagattaaga atgattctga attagtgaat atagttagac aaaattttga agttttgggt
    10621 gaagactatg ttgaaagaga tttaagtcaa ggtataggtt caactgatac aggaaattta
    10681 actcatgaga tacctgcaat acaagcatat ataaaattaa aagaaaatac tgcaactcat
    10741 acagatgaat ttgctgttgc agctggtggt gaagaaggca aagtagcact tataaaagca
    10801 attaaggtgc tagcaatgtg tggagtagat attttgtata gtaaataata tataatttta
    10861 aaatatatta agaaaggaaa tcggattaga catgatatta aaacaagtaa tagaggttta
    10921 tgacatttta gataaagcaa atgctaatgg ggaagaagta aaaacttact tacaaggtta
    10981 tggtgaagta gatgtaactg taaaagaatt gtcttcaagt aaaggttcaa cagatttagt
    11041 aaagcttaca attcctggga aaaatggaaa attaaaaggt ggagatgctc ctactcttgg
    11101 tattttaggt agattaggag ggattggagc aagaccagaa gttataggtt ttgtctcaga
    11161 tggtgatgga gcattggtag caattgcagt ggctgctaaa cttttggata tgcaacgaaa
    11221 aggggacata ttagatggtg atgttgtaat ttcaactcat atttgcccag atgctccaac
    11281 taaagaacac tatcctactc cttttatgga ttctccaatt gatatgatga ctatgaatga
    11341 aaatgaagtt gatagtagtt gtgatgcaat actttctata gatactacta aaggaaatag
    11401 agtaatcaat actagaggat ttgcaatatc gccaactgtt aaagaaggct acatattaaa
    11461 aactagtgat aaccttttag atattatgca gactgtaaca gggaaatctc catttgtatt
    11521 ccctttgtct attcaagata taacaccata tggaaacaat ctgtatcact taaatagtat
    11581 tttacaacca gcagtagcta cagatgcacc aatagtagga gtagcaatta caacagagat
    11641 gcctgttgct ggatgtgcta caggtgcaac taattttagt gatttagatt cagctgggag
    11701 atttgcaatt gaagttgcca agctatttgg tagaaataag tgtgatttct atgataaaga
    11761 agagtgggaa atgcttatta agagatatgg caaattaaat agatttcaaa catttggtat
    11821 acagtaatta gaaaaatatt aagtttatat aatttgtgat acaactattt aagaagatat
    11881 aaacaattag gaataaaagt aagaataaag cacttagtta aaaactttaa aaaaaaatat
    11941 ttatttgttt atataaaaag gaggattctg gttattggga ttctcttttt tatgaataca
    12001 aataatatat tgtaattata aaaaaaggag cgtttcaaag tgattttttt aatcaccttg
    12061 agacaactcc ttatattttt tatttaaatt ctatttttta ttgactgtat agctattttc
    12121 accatcataa ggtggcatga ccttgaacag cgtggttttg taaagttggg caccacaaaa
    12181 caaaaaatca acaagccctt gtgtggtctg ctgacgtgtt tactatacta tactttccca
    12241 aaaaccgcaa gcactatggg aggctataag gcggcgacca ttcaggggcg atcccctcga
    12301 aaccgggata gggttcccat gtgggacata ataaagagcg ccccggaggt atcaactacc
    12361 atccggggcg ctctttatta tgtcccgtgg gaaccatgcc cacgaagcag ggcgtacaac
    12421 ggacagatag gtcctaccct cctgacagac tggaatctca atcccatcga aaacaatcat
    12481 aacaatcatg gcaagattat atacactcct gtaaaatatt tttgtgtgta ccatgaagta
    12541 ctgcttgcca ctattgagaa cggataaaag ggaagtacgt attataccaa taaggtgaac
    12601 gaaacaaaat actcatttga taaagttaaa taaacctgtc ggaaccccac caatccggca
    12661 agagctcact caatttaaca gttttttctg tttcgacatt aactaaaatc tcaatacaag
    12721 cactatcctt atcaagttgc attaaatatt cacgacaagc accacatggt gagccaacac
    12781 tcccattgct catcacacaa actaatttag taattttact ttcgccattt gttatcatat
    12841 tggcgatagc atttctttct gcacacattc ccaatgtaca ggctgtatca atacacacgc
    12901 cagtataaac atttccgctg tcggtaagga ttgctgcggc aaccccacca gcatcgataa
    12961 aaggtgaaat tgtgcgtgga tttaattttc ccctagcatt attataaagt tccttccatt
    13021 tcatcaggtt gatcctcctg ttgtatccat ataaataatc ttttatacgc cttttccgcc
    13081 taaggcacac aacacattac tcgctttttt cattctaata ttcgtgcgtg aaaatgtcaa
    13141 acggtttact tcgtttcatt acttcatact gctgttcata accctgtttg ttcacttggg
    13201 caccattcga gcaccaattt acccgtttca gggttgtagc gaacacagca gaaatgctca
    13261 tttcatgggc ttttctggct agaaaaaaaa ttttatcgct gccagatatt tactattttt
    13321 tattaagttg ctggagttcc accaccatca ggaggagtag tagagccacc accattattt
    13381 cc
//
LOCUS       DAMKPG010000096        13158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMKPG010000096 DAMKPG010000000
VERSION     DAMKPG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.101"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            234..1115
                     /locus_tag="PC621_002966"
     CDS             234..1115
                     /locus_tag="PC621_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4110109.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1323..2186)
                     /locus_tag="PC621_002967"
     CDS             complement(1323..2186)
                     /locus_tag="PC621_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF4110110.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2341..4275)
                     /locus_tag="PC621_002968"
     CDS             complement(2341..4275)
                     /locus_tag="PC621_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF4110111.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4387..5400)
                     /gene="galE"
                     /locus_tag="PC621_002969"
     CDS             complement(4387..5400)
                     /gene="galE"
                     /locus_tag="PC621_002969"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF4110112.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5425..6294)
                     /gene="galU"
                     /locus_tag="PC621_002970"
     CDS             complement(5425..6294)
                     /gene="galU"
                     /locus_tag="PC621_002970"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF4110113.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6492..6812)
                     /locus_tag="PC621_002971"
     CDS             complement(6492..6812)
                     /locus_tag="PC621_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110114.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6802..8793)
                     /locus_tag="PC621_002972"
     CDS             complement(6802..8793)
                     /locus_tag="PC621_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110115.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8915..11458)
                     /locus_tag="PC621_002973"
     CDS             complement(8915..11458)
                     /locus_tag="PC621_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF4110116.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11736..12070)
                     /gene="rnpB"
                     /locus_tag="PC621_002974"
     ncRNA           complement(11736..12070)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC621_002974"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12202..12879
                     /gene="srtB"
                     /locus_tag="PC621_002975"
     CDS             12202..12879
                     /gene="srtB"
                     /locus_tag="PC621_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF4110117.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(13039..13113)
                     /locus_tag="PC621_002976"
     tRNA            complement(13039..13113)
                     /locus_tag="PC621_002976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13079..13081),aa:Thr,seq:tgt)
BASE COUNT         4061 a         2300 c         1337 g         5460 t
ORIGIN      
        1 ttcctattaa ttcatagatt ttgtttatag attttattta taagataatt cttgtatttt
       61 tctattaggt tgtataaata tatttatctt ataattcaga caaaaaacga taaatttttt
      121 taaaagcttt tattcttact ctataatcat atataataaa aatgtaaaaa tatcttaaag
      181 gttaacttct acaaatatat aaataaaaat acctatgaaa ggatgaacaa aaattgaatt
      241 tatatcactt acgctatttt gttacattgg cacatttaga acactataca aaagcggcag
      301 aaaatttatc tataacacaa ccaagcttaa gtcatgctat ttctttacta gaaaatgaac
      361 ttggggtggc tctatttgaa aaagaaggta gaaatatagt ccttactaag tatggaaaaa
      421 tatttttaaa agatgtggaa aaatcattag aaattttaga ttcaagtgta aaatcattaa
      481 aaataactgg tactggtgaa ggacaaatag acttagcttt tcttagaaca cttggtactg
      541 attttatccc agacatagtg cataaatttt taaaatctaa tcctgctaaa tcaatagatt
      601 ttaaatttca tactggtgta actactgaca ttattcaagg tcttaaagaa agaaaatatg
      661 atattgcatt ttgttcgaaa cttgaaaaag aaaagggaat tgaatttata cctgttgcca
      721 aacaagattt agtactaatt gtaccatata gtcatccact agctgcaaag gatactatag
      781 atttaaaaga aaccatacct tatcctcaaa tagtatttaa tcagagaagt ggtcttagat
      841 atataattga tgatatgttt aaaaaaataa atcaacagcc taatatagtc tatgaagtag
      901 aggaagacca agtaattgct ggattagttg caaaaaattt cggaattgct gtagtcccaa
      961 atatgaatat gcttagcttt acaaaagtaa aagtaattca gattattcac ccaagttggg
     1021 aaagaaactt ttacttagca tttataaaag atagatattt gcctcctgca attaaaaact
     1081 ttaaaaactt tgtcattaaa aacgctcaat tataatttat taataaaggg gttattttta
     1141 aatatcctct tttattcttt ttataaaata aaaaaatcta tacttttaaa agtatagatt
     1201 cctaactgac agtcggcatc cgagtctcag taattatcgt tatacaacag caactggatt
     1261 cagttcctga ataactaggt tatttacatt atatcagatt tttttaaatt tgtcaattgt
     1321 atctaacgca ttttttcaag ccatggtcca acaggctcta caataggaac ttctttaaat
     1381 ccccaatttc ttatatcatt cacactcgtt ggagtatata ttttgtttct gacagatttg
     1441 aatattttgt ttttttctcc atattgcata cactttggat tagcttctat taaccctctt
     1501 cttgctctgc taagaggatt acataaaata tctgctattt ctaaaccttc tctatagtca
     1561 gcatcttctt ttttggcaat tataaaaccc ttaaccttat ctcttaattc ttcatctata
     1621 tctaagtata ttgttccatt attaaataca tcaaaaaacg ccttttgtac ttttaagttt
     1681 gagacatcat cccttgactc tataactatt cgtgcagatt ctacagtttc attatttaca
     1741 aatgaataaa aattctgaag aacatgtata aaagccacaa catacggttc ctttgaaggt
     1801 gcataatatt tctttaatct agctttatct actgttactg atattatagt acactctaat
     1861 ctagatagaa ataaaggcaa ctcatcatag aataacttaa tctgtgaatc atcaaacttc
     1921 tcaaatgctc ttgattttct tgatatatct gtcaaatgta ttcctatgtt actggttcca
     1981 aaacaatact ctttaaatgc atccatttct ttcctcaaag aacatacata atcgccttga
     2041 cttactgcat atttatattc aaaaattact cctgtcaatg taaatgggtc ttcatgattt
     2101 atatcagctc ttccactttc atcaataaac attatatatt ttttctttat aatcttactc
     2161 accctcttaa catatgatga tagcatctat attcatttta tatgagtttt aaaaatattc
     2221 caagattttt atataactta aacttaattt tatactaaaa aattgagatg taaaacaatc
     2281 aagcttttat tgattgaatt tcacatctca attataatta tttttcaata tatattttta
     2341 ctattttgca agaatatcca ttaatttatc cattactata taagatactc cgcctcctat
     2401 ttgataagga gtttcaactt ttttatttgc aaatactctt tgttgctcta atgataatcc
     2461 tgaattatcc attaaaacta ttggtgaatg taattttaca gctaatggtc cagctgataa
     2521 agcatctact aaagcatctg atttactaac taatacagcc tgtaaatcag tatctccata
     2581 gaatttttct attactttgg cattagtatc tactctacta tctccatata ttctatttcc
     2641 tgaaatatct tcagtagtta tatcattcat ttttgctatt actgaatcgt taattgcact
     2701 tggtccacct ataaagtatg cattttctaa ttttctttct gttagccatt tataagattc
     2761 ttctgtaatt ccatctttat tgactaatat tataggttgt ttttcttccc cagctttaga
     2821 agctacagat aaagcatctg cttccccatt tccagaaacc atatatgcag tttttatttc
     2881 ttctcgattt ccaagttctt cagctattct tgtagcagtt tgatatctat cttgacctgc
     2941 tattctcttt gtctctgtgt tgaaagtagt ttttatttct tgctctaatt tagaacttat
     3001 tgcattttct ccacctatca agattactgt tttaggtgct aataacttca tttcttcaac
     3061 agtttcagtt ggtaaattag ctgtattagt aagtagtatt gttgagtcat agctacttgc
     3121 taaaggagtt gctgttatac catcagctat tgcattacca tttactaata ttacagtatc
     3181 tgaactatca gcattccagt ttccaactga acttatcttt ttagctgtat caaatctatt
     3241 tgcacctata attgtatccc atgtaggatt tatagttcca tcttcattaa aagtataatc
     3301 tttcccatct attttttgag aaccagttag catctttcca tctttgtcta aatagtaaga
     3361 cttaccatct atttgttgcc atcctgtctg cattattcct gttttgtcaa agtaatactt
     3421 attaccatct atttcttgcc atcctgttac tgataaacca tcagatccta gatagtattt
     3481 ctttccattc ttttcttgcc atcctgtttg cattactcca tctgagttaa agtaataagt
     3541 ttttccgtct attactttcc atccagatac tcttgctcca tcagttccta aataatattt
     3601 tttgttatta attgctttcc atcctgtttg cattactcct gaagcatcaa agtaatacat
     3661 ttttccattt acttttagcc aactattctt ttggtatgtt ccatcagatt ttacataata
     3721 cttcttacca ttttctgttt tccatcctgt ttgatttaga tttatcttat attttgccgc
     3781 tatctttaca tagctgtcat tactataatc tataattaca tctgaaccta caggaacttg
     3841 gtcaaataac catctaacat ctttattgtg cattcttata catccaccag aaatatgctt
     3901 tcctatagaa ctctcattat tatttccatg tattccatat gtatctccat aagttccttt
     3961 taatgctaat cccatccatc tatctcctaa tggatttctt ggacttcctc caggtatatt
     4021 acctttataa tatggtctat tttttatctt gttaactact tttgttttcc ctgttggtgt
     4081 ttctgacccc tttttacctg ttgcaactct aaattctttt acaagtttat tatttacaaa
     4141 atatcctaga gtattctttc ttgaatttac tataatcaca tgtttactgg ctgcgcttga
     4201 ttctggtacg ccacatgcaa atactaaaac taaagtcagt gctagtacta aatacttttt
     4261 tagacttttt ttcactataa cttccccctc ttttagaaac attactacat gtgtctaatt
     4321 ataacataaa attaccaata ccttatattt acaaatttgt tacatttata catataagta
     4381 tgtattctac tcttcatacc cattaggatt catactttgc catctccatg aatcttcaca
     4441 catctcttca agttcatact tagctttcca tccaagctct ttttctgcct ttgaagaatc
     4501 tgcataacac atggctacat ctccagccct tcttcctaca atcttatatg gtatttcttt
     4561 tccacttgct ttagaaaatg cttttactaa atcaagaaca ctataaccct ttccagttcc
     4621 taaattataa acaacaagtc ctggattttc ttctaatttt tgtaatgctt ttacatgacc
     4681 agcagctaaa tctaatacat gtatatagtc tctaactcca gttccatcat gtgttggata
     4741 atcattacca taaacactca attccttcaa tttacctact gctatttttg ttatataagg
     4801 cattagattg ctcggaactc catttggttc ttcacctatt ctaccacttt tatgagcacc
     4861 aactggatta aagtatctta aaagagctat atccaatgac ttatctgctt ttgatatatc
     4921 tactaacatc tgctctatca taagtttagt tcttccatat ggattggtta cagaaagtgg
     4981 gaaatcctct aatataggac aagtatgtgg gtctccatat acagtagctg aagaactaaa
     5041 tacaaacttt ttaacaccat attctctcat taattcaaat aaatttaagg tacttattag
     5101 attattgctg taatattcaa ttggtttttc tactgattca ccaactgctt ttaaagctgc
     5161 aaagtgtata attgattcaa tattattttc tttaaataca acatgcattt catctttatt
     5221 tctgatatca atattataaa attttactgg ttttttagat aattccttaa ttctatcaac
     5281 cactattgag ttgctattac ttaagttatc aactataaca acttcataac ctgattctaa
     5341 aagttctatt gctgtatggc ttcctatata tcctgctcct ccagctacta aaactgccat
     5401 tgtaattacc tcttttctaa tattttattt ttcacttact ttttttctca tatagtccat
     5461 taaatcatct tttaaatttt ctctctttag agcaaagtct attgttgcct ctaaaaaacc
     5521 aatcttatct cccacatcat atcttctacc ttcaaagtta taagcataaa tagcctcttg
     5581 ttttccaaga gtttgaagtg catctgtcag ttgtatctct ccacctttac caggtgtctg
     5641 tttctctaat atactaaata ttgcaggagt tattatatat cttccaagta tagctatatc
     5701 agatggagct tcttcaattg caggtttttc aaccatatct ttaactttat atactctatc
     5761 ttcgatatgt tttacatcca atataccata tttattagta tcttctttag ctatcttttg
     5821 aacacctaat acagttgttt tatactcatt ataagtatct attaattgtt ttaagcacgg
     5881 aacatctgaa tctactatat catcacctaa taatacggca aaaggttcat cacctataaa
     5941 actttttgca cagtaaattg catgtccaag accttttggc tctttttgtc ttatataatg
     6001 aatatttacc atatttgata tgtctcttac catttctaac atctctgtct tacctttttg
     6061 ctctagttct aactctaatt caactgattt atcaaaatga tcttctatag attttttatt
     6121 tctacctgtt attataagaa tttcttcaat tcccgattct atagcttctt ctattatata
     6181 ctgtaaagtt ggtttatcta ctataggaag catttctttg ggctgtgatt ttgttgctgg
     6241 taaaaatctg gttcctagcc ctgctgctgg tattattgct tttttaactg tcatctgcat
     6301 tttttcaccc ttcttcgtaa attttctatt gtaccaaatt aatataccat atttatactt
     6361 tttcttcaaa ctctatatta tcccaatatt taatgctata agataatata aggtagatga
     6421 aatttcatct acctttagtt ttataagtta atattgtatt gtttatattt tatccaaaac
     6481 ttatacaggt actaaatttc atctaagatg ccttttatat cactatctat atcttcatct
     6541 agataagaat ctaaatcttc taagtcaact tctagttctt gatatgtcat atcaacactg
     6601 acatcctcat ctatatccat ttctgatatt aattctccta aaatactatt tccatcagaa
     6661 tctaaaccac tatctatatc ttcatctata tagttttcat ttaaaatttc atctaaatca
     6721 atctcgtaat aaacttcatt atttttttca tcccctgagt ccaatcctaa gaactcatag
     6781 ttaaaagtcc aaacattgta tttacttgac atactttatt cctcctgtga aaaacatatt
     6841 ttcatctagt ttattggttc cataatcaac agagttatat attttttcaa ttatgtcttt
     6901 tcttctttct tttgtactat tatttaatgt caattgttta aatacatatg tatttgttat
     6961 tctctcgaat aatatttctt ttatactatc atcatcaata tttctatagt ttttccacaa
     7021 taactcaaat aacttaaaca tttccctact agtttcatct acataataag gatacatatt
     7081 ttctactaaa tggtttaatc ttgatatggc ttgttttaaa aatacatcaa gacttgcact
     7141 ttcactttct tttaacattc tataattgta tttcttctta tcttctcctg aaattacaaa
     7201 gatgatattg tctaaacttt tttcaacttc atttaaataa ctgcttatat tatatgataa
     7261 gtcagacaat agatttttaa tctgcttaaa cacttgtaca tcaagtttag tattatactt
     7321 ttcaaacaaa tctatttctt gattttctaa tattcttggc atatatgctg acaataaatc
     7381 taaattgttt ctttgttctt gtatttgctt ataaagtgaa tttataactt ctattcttat
     7441 ttcatcattt aactcttttt ctaaaatctt atcaaattca gatttcatat agtaaatatt
     7501 tccaaaaaaa ttaaatatta tcatatcatt catagcttta gaccaattct tatatatcac
     7561 ttcaatagaa ttttctttaa gtagaggttg ctttgcatat ataaatacat cttgcataag
     7621 tttttgaacc atatttttac ttaagtttaa aagtactttt tgattattaa attttatttc
     7681 cgtttctaca aaattagaat tttcgttttc aagttcttta ttaattacaa tgtcttttat
     7741 atcactattt tttatagata tgatttcttt ttgaaaagac tttagaatag tcaagctatc
     7801 gaaatcttct tgaataaaaa atggagcatg ttttaatgta tatcccacta tttcattttc
     7861 tatatctttt aagtcatcta tttgcataat ttctctattt ttatcattta tatgtaggtt
     7921 aaatacattt ttattatcat ataatattat atcttctcct aaatttaact tagaacaatt
     7981 atctttcgct gactttacaa tagtcttctt agatttctta tttataaaaa ttaattggtc
     8041 ttgatatttt actaaaagca tgtttgtgtt tttataatca aatccatcta tatttcctga
     8101 tatcttgcag aatactatat cacaatcttt aggaagattt cctatttttt ggttatcttt
     8161 aactctctca tttaaatcag atggaactac actatctata ttgttattaa atatagatac
     8221 agaccttact ataatcccct ctctttccat atgaaagtat cccttcaatg acactctttt
     8281 atcttctaaa atgtatcttt ctatatcatc aaaatttatt tcaaatctat ttttcccact
     8341 gataaatata aacttctcat tatctttatt aattccaccc atataaaact tattgtcatc
     8401 taaacttaat atagtcatat tatcctttag atagaatata aattctcttt gacttttgca
     8461 gattaaatca tattcatcca aagttatttt catagtcaaa tttgctgtaa catttttact
     8521 tgaattttca ctttttatgt aaaaaggcaa gttatttcca tagacaatta tttgagaatc
     8581 ttctaaaact gcctctaatt tttctttatt gtctgaaaaa ctatatatat attttacacc
     8641 tgtatcctca ttaacttcat ctgatttgct gattattata ttccctagat ttgttatatg
     8701 aagcttttta tattcttttg agtatctact tatgccacta agctcaaacc catcagaaag
     8761 ctcatatatg attttttctc ctaatttcaa cataccttca ccacctaaac agaattataa
     8821 gtattttata aaggtgaata aaatgtctca aaacaaataa tattttttta atacttttta
     8881 ttctgagaca tagtttctcc ttttttataa tgctctaagc accttcaact atttcttcat
     8941 aaagtttatc taaatgttta acatattcac gatatccaga tttgttcttt aactcacttc
     9001 ttaaatcttc atatttttgt ttaagttctt cagcactcaa agaaggagct gattctttta
     9061 attcttctaa cttataatca tagtacttat catacatagc tatatatgta tcaatagatt
     9121 tcttagctag ttcattacat ttttcttgtg cttctccata ttgagaataa tcacctattt
     9181 ttattttata gtatccattt tcttgaatta ttgttgaaga aataccttta gcctcatatt
     9241 cttttgctgt ttcttttgct gttttttcaa ctttaagtga acctgcataa acagtgtaat
     9301 atacaccttt accatcttta tatgcttcca tttcttttgc tttcttttca gcttgtgctt
     9361 tttcttgagc ctctttttca gcttttgcct tttcttctgc ttcttttttc tctttttcag
     9421 cttgtgcttt tttctcttcc tcagttaatt tattgttagc ttgttgttga gttgatgact
     9481 ttggaatatt ctctttatca tttttcttat tgttgcctac tgttataaaa tatcctacac
     9541 caacaataac cactataact actgctataa tagtttcttt taatctactc ttcttagaaa
     9601 caacctcttc atcatctaaa gaatcttctt tatctgatac ataatttttc atttctttct
     9661 tgttttctct atatttgtcc ttagaagaat ttttagattt tttattacta ttttctttct
     9721 ttttaatagg tattactact gatttttcat tttctaattt ttcttctttt atttcttttg
     9781 cttttttatc ttctaagttt ttactttctt tttcaagctt cgattcatta tttgaagata
     9841 ctttttcatc taagttatta tttattggtt tttctatttt tacactacgt tcttcttcta
     9901 tatgttgact tggttcttct tgttttattt cttttaattc attttgctct atttcttctg
     9961 attttgcttg atgattttca actggtttat ctaaaattat atcaggagaa atatcattta
    10021 aattctcttc ttcttgttca acttcttcat tgtctttttt aataaatcca aataatttag
    10081 ccttcttttt actcttttta ttttctggtt caattatatc ttctttattg ctgtcgtact
    10141 ctttatctgt agtatcttta tcttctacaa cgtcacctat atcttgatta ttactatcta
    10201 cttcttcttt agccccttct tcagcatcat cttctattga aatactactt ctaaagcaaa
    10261 tatctttatc tgcatttttt atcatgtaaa tgtattcttc aatatcgata tctttatttc
    10321 ttctagttct ttttgatgca atttcatgat tttctaattc actatagtta tcttttatta
    10381 gattctgtgc tttattccat gtatcttcac taatttctgt tttattttta attacatagt
    10441 ccacaaattc cttaaaattt tcaggaccaa attctccaat tatagtcagt ctcattgcac
    10501 cttctataag gtctctattt cttctcttta aaaattctaa tacaaccgtt gcacaagcaa
    10561 aatctttaat aatttctttg ccaaaaccat atccttcaac agaatgaagt tcttctaatc
    10621 cacataactt cttatattca ttgtgtattg tttgagatat actttcatta tttaagctat
    10681 ttaacttatc tataaacttc gattctatat catttgattt tgattcatgt tttacttttg
    10741 aatttttaat ttcatttagt agcatgtcat tttctgaatt atctttacct aattgcaaat
    10801 cctcaatttc agttgctttt atttctactt tgtcttgttc gttattctct tccacattat
    10861 taggtaccac atctttattg ctagctaata ttttgtttac acagaatata atttctcttt
    10921 ttaattctct tttcgcatct gcttgatttt taagaaataa atctccacat tctttctttt
    10981 ctaaacttat ttttctaaga gatatattaa ggaggtcttt atataggttt aaaaccttat
    11041 aatccttttc taaacgtctt cttctctctg atagttccat tttatcataa gctaaaaaac
    11101 tatttaaatc attggctttt agagagtcta atccctcttt catagcctta tttacatact
    11161 ttctacattt tgctgtagtt aattctaaat ctttttctaa ttcatctatt tcaggtatgt
    11221 acttatctac tatccatttg ccaataagtg gttctgtatt tcttgctaat gtcttataaa
    11281 acaatatatt aaattttaaa agctctgcat attttgcttc attaaattct actttttcaa
    11341 atttcttttc caaaacacca tctagctttt catttatttt agactcttgt tttaaaaaac
    11401 ttgctgtgac atttaaaaca gtgtttctta ttatattatt ttcactcaaa accttcactc
    11461 cttaaatcaa ttattttatt gtaatattta cattgtgcaa agttgctaat ttgattatga
    11521 tatctaaata attatttact aaatttgcta catcttatat catatcatat tatttacatc
    11581 attttacttt ttctttttat atctatctta tattatacca tttttaatat ctgtactaac
    11641 ttatattaca atattcctca aaattttaaa agtagattag acaaatttac aaataaaaaa
    11701 acatcagttt aaactgatgt ttttgactat cgaaataatt aagcaagtct ataagcggag
    11761 ttctgtatta gataatcatc tttctaggcc tattgtcacc aataggctca agcgacctac
    11821 ctaccggaga gtacgagcag cactctttat tcctatcttg gtcttgctcc aggtggggtt
    11881 tacatagcca actagtcgcc tagctgctgg tgagctctta cctcgccttt ccatccttac
    11941 cacaatagtg gcggtttatt tctgttgcac tatccttagg atcgctccca ctaggcgtta
    12001 cctagcaccc tgctctatgg agctccgact ttcctcatgt aagattacat gcgattatct
    12061 ggcttactta atatttattt tacttagtta tgttaacaca aattctttta catgtcaaag
    12121 taatattata ctaaaaattt aaatttattc aaaaatagtg taatatagat atataaactc
    12181 catttaactc gaggtgatat attgaaaaaa ttatatagaa tagtaattaa cattatacta
    12241 gtattagtaa tactttacag tggatttaat atatactcaa aacttaccaa atacaatcat
    12301 gatactaaaa catcctcaga acttcaaaaa aaagaatata aaaaagagga tttatcaaag
    12361 attaacagtg attttaaatt ttggctttct gttgagaata caaatataaa ttatccagtt
    12421 gtacaatcta aagataattc ttactatctt gacaaagatt tttataaaaa agattctatt
    12481 tctggtacac tgtttatgga ttatagaaat aaatctattg atgacaaaaa tatcataata
    12541 tatggacata atatgaaaaa taaaactatg ttcaacaacc taaataagtt taaagatgct
    12601 gacttcttta aaaacaataa taaaataaaa ataaccttga atggaaaaga atttttatat
    12661 gatgtttttt ctgcttatat tgtagaatct gattatgatt atctaaaaac aaattttaac
    12721 aatgaatctg attatcaaaa ttacataaat gatattactt ctaaatcatt gtataaatct
    12781 cctattaagg taaatagcaa tgataaaata gttacacttt ctacatgtac ctatgaattt
    12841 gatgatgcca gaatggtgat tcatggtaga ttgatttaaa actcacagac acagtttaaa
    12901 acaataattt taaactgtgt catcttttat acctataatt tataaattag gccactaaaa
    12961 gtatgtaata caaaataaca aatacatcaa acaataaaaa acaataaaaa aagactatgc
    13021 taaaaacata gtctttactg gagctggtga taggaatcga acctacaacc tgctgattac
    13081 aagtcagctg ctctaccgtt gagccacacc agcacaccta ttctattgta aattagtaaa
    13141 actaattttt aaaagtta
//
LOCUS       DAMKPG010000097        13047 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMKPG010000097 DAMKPG010000000
VERSION     DAMKPG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.102"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            77..706
                     /locus_tag="PC621_002977"
     CDS             77..706
                     /locus_tag="PC621_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110118.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            681..1085
                     /locus_tag="PC621_002978"
     CDS             681..1085
                     /locus_tag="PC621_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF4110119.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1088..1561
                     /locus_tag="PC621_002979"
     CDS             1088..1561
                     /locus_tag="PC621_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF4110120.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            2108..2773
                     /locus_tag="PC621_002980"
     CDS             2108..2773
                     /locus_tag="PC621_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF4110121.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            2795..3727
                     /locus_tag="PC621_002981"
     CDS             2795..3727
                     /locus_tag="PC621_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF4110122.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            3748..4389
                     /gene="pcp"
                     /locus_tag="PC621_002982"
     CDS             3748..4389
                     /gene="pcp"
                     /locus_tag="PC621_002982"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF4110123.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(4508..4840)
                     /locus_tag="PC621_002983"
     CDS             complement(4508..4840)
                     /locus_tag="PC621_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110124.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            5106..5726
                     /locus_tag="PC621_002984"
     CDS             5106..5726
                     /locus_tag="PC621_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4110125.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(6051..6875)
                     /locus_tag="PC621_002985"
     CDS             complement(6051..6875)
                     /locus_tag="PC621_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF4110126.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6886..8289)
                     /locus_tag="PC621_002986"
     CDS             complement(6886..8289)
                     /locus_tag="PC621_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110127.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(8293..9018)
                     /locus_tag="PC621_002987"
     CDS             complement(8293..9018)
                     /locus_tag="PC621_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110128.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(9130..9903)
                     /locus_tag="PC621_002988"
     CDS             complement(9130..9903)
                     /locus_tag="PC621_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110129.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(9909..10796)
                     /locus_tag="PC621_002989"
     CDS             complement(9909..10796)
                     /locus_tag="PC621_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110130.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(10810..11832)
                     /locus_tag="PC621_002990"
     CDS             complement(10810..11832)
                     /locus_tag="PC621_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110131.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            12407..12583
                     /locus_tag="PC621_002991"
     CDS             12407..12583
                     /locus_tag="PC621_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110132.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4802 a         1897 c         1510 g         4838 t
ORIGIN      
        1 tattgacagt attaaaatta aattatacaa taacagtgta aattaactta acaccgttaa
       61 gcaaagaggt gattatatga gtatcaaaga aagacgtaaa aatgaaaaag aagaaatgaa
      121 gaaaaagatt atggatgcta gcatcgaaat tataaaccaa catggatatg aaaatttatc
      181 aataagaaaa attgctacaa aaatagaata ttctccaact acaatttatc tttactacaa
      241 ggacaaagca gagattatca gcgatatgac caataaactt tataacacag tagaaagtaa
      301 tgctattgac attatgaata attgctcatc actaccaata gataaacaaa tcatagaaat
      361 tatgactagc tttataaaaa ctctttctag tgaaccagag atggcaaaag ctatcatgca
      421 tagtagaatg aatattatat ttgcatctga aaatacaaac aatactccta gcaataatgg
      481 tataaaaatg cttgataaat tcctttccat tggaattgaa caaggaatat tcaaaaaaaa
      541 tatagataaa agctcatgga tgcttataag tgcattgtta ggattcgttt tatgtgttgt
      601 tgaaaataaa ctatattctt tacataattt ttctcaactt atagaaaatt tcttagatat
      661 tttagtggga ggactttaca atgaaaattc gcaacaaagc tactaataaa aagaaaaaat
      721 atgtaaaaat atctattgct ctattctttg tatttatatg cattattatt gtaattgtaa
      781 gcaacttaaa gccagaaaac ttggtagtaa aagatataga tattaataat gttaaaaatg
      841 gcatttatac tggaagtgct gataataact tggtaaaagc tactgtgtct gttgaagtta
      901 ataatggtaa aatccaaaat attaatatct taaaacacga ccatctttta ggtaaacctg
      961 ctgaaaaaat tacaacaagt attattaaac aacagtcttt agatgtggat gctataactt
     1021 ctgcaactta tagcagtaat actatacgaa aagctgtaga aaatgcttta agaaagggag
     1081 aataattatg aatactatta ttatatattc aagtaaatat ggttgtacaa aagactgtgc
     1141 aaatatacta aaaaataaac tatcagataa tgtcactatt gttgatatta ataataataa
     1201 taaaattgag ttatcaaaat ttgataaaat tattattgga agttctatat atgttggttc
     1261 agtttcaaaa aaaatacaag tgctatgtaa tgataatgta gagttattaa acaaaaaaca
     1321 agttggcatt ttcctttgct gtggcttttc tgaacaagca gataaatatt taaaatcaaa
     1381 ttttccatca tccttattag aaagtgcaaa tgctattgga atttttggta gtgaagctcg
     1441 attagaaaaa atgaaatttt tagataaact tattatgaag gctgtttcaa aaggtaatta
     1501 tgacagtttt agaatatcac aggataatat agataatttt ttaataaatc ttaatagcta
     1561 aaataaaact aattatttct atgataaatt cttttattta taagtaatat tgctcaaaat
     1621 agtctttaaa ctttatcaaa attttatttt tcttagtgaa atctaatgaa actagctctt
     1681 aatatatgct ttattaatca taacaaaaag agagtgtcta atgaactaaa aagttcatta
     1741 gacactctct ttttgtatga aatcaaatat atttttatca tttcaaaaga gaaaaataca
     1801 cttgtctcta tgtttgacac attctctttt tataatataa tatctaattt aaaacctatt
     1861 tcaaatattg acatatactt aaaccataga cttacaaaat caattcatat tagcttaaaa
     1921 tataatcttt gttactcaaa ttcctataca caaccattat ttactttgat actttaaaac
     1981 atcttcccta ttttttatta aattttataa taatagtcct attaacgcaa atactataaa
     2041 aagagtttaa ctttatccat taaataaatt taaattaaaa catatagaaa tggaaggaga
     2101 acaatttatg ataaaactaa tcggtatatt gatagttgta attggattta tacttaaaat
     2161 tgacacatta tttactgtat tattagcagg agtggcaaca ggaattgttg caggattaga
     2221 ttttaatcaa atttttacaa ttttaggaga ttcttttgtc tcaaacagag gtgtatcatt
     2281 attcatatta acactgccag taatcggtgt attagaaaga tatggtttaa aacaaagagc
     2341 tgtttcttta atagaaaaat taaaaagact tacaacagga aaagtactta ctatatacat
     2401 gatagctaga caaatagctg gtgctctatc aataagaatg agtggacatc ctcaatttgt
     2461 aagaccactt gttaatccta tggcacaagc tgctggttta agtaatagtg atgaactaaa
     2521 agaatcggat gaagaagcca taaaggcatt atctgctgca tcagaaaact atggaaattt
     2581 ttatggtcaa aacttatttg caggtagtag tggagtatta cttatagcat ctactctaac
     2641 tcaatttgga tacaatgtaa ctggacttaa tattgtaaag gctagtacta ttatggcagt
     2701 aatagcactt gtagtggcta caatccagtt taccttatat gataaaaaat taaacaaaag
     2761 tcataaaaaa tagttttatt taggaggaat aaaaatgcaa aattttataa acatatcatt
     2821 ggaaatcttt tacgcactaa tgggattttt aatgattgtt atagcatata agtcttttac
     2881 taccataaat aacaataaga aatatggtac atcattattt tggatactta tatcactgcc
     2941 attcatattt ggtagattga ttccagccaa tataatagga ataatattaa tattatctag
     3001 tttattaaca ctttctaaac aagttgtatt tgcaaagtat gaagaaccag atgaaaattt
     3061 tggaaaagaa caagcagaca aacttaaaaa taaaatattt ataccatcat taatattagc
     3121 atttgctgca gtagtagtag ctatgagcct ttctaatttt gataattctt ctcaatttgc
     3181 aataggtgtg ggttcaataa ttgcattaat atcagccctt ataataacta aagcaaaacc
     3241 tgctacatca gttcaagatg gctcaagatt acttcaacaa atgggacctg ctagtatgtt
     3301 accacaactt ttagttgcat taggagcttt atttactcag gctggagtcg gcgaagttat
     3361 ttcaaccatg atatctggtg tagtccctgc tgatagcaga ttatttggtg ttatagctta
     3421 tgtattaggt atggttatat ttaccatgat aatgggaaat gcatttgccg cattttcagt
     3481 cataacggca ggaattggta ttccttttgt attgtctcaa ggtggaaatc ctgctataat
     3541 tggagcatta gcattaacag caggatattg tggaactcta cttactccaa tggctgctaa
     3601 ttttaatata gttccagcag ccttgctaga atgtaaaaat gattatattg ttataaaata
     3661 tcaagctcca gttgctttgg tattaattat agcccacata ttagtaatgt actttttagg
     3721 attttaatat aattaggagg taaaataatg aaaattttgt taacaggatt tgacccattt
     3781 gggggagaac taataaaccc agctcaagaa gcagttgaaa gggtcaataa taatataaac
     3841 ggtgcagaaa tcataaagat aactatacca acagttatga caaaatctgt tgaagctata
     3901 gataaagcta ttcaagagca caatccagat atagtaatat cagttggaca agctggaggt
     3961 agatttgaca ttacacctga gagagttgct ataaatatag atgattttag aataaaagat
     4021 aatgaaggta accaggttat agatactatt ataaaagaag atggtgaacc tgcatatttt
     4081 tcaaaattac ctgtaaaagc catggtaaaa catatgaatg aaaataaaat tccagctagt
     4141 gtttcaaaca ctgcaggaac attcgtttgt aatcatgtga tgtatggaat cctttatatg
     4201 atagacaaga aatatcctaa tataaagggt ggatttatac acatcccata tacaacttca
     4261 caggtaataa ataaaaaaaa tacaccattc atgtctttag aagaaatagt aaaaggttta
     4321 gaattagcta tagaagcttg tattatatac aaagaagatg taaaagaaat tggtggtgaa
     4381 atttcataat aaatttttta gtattttata aattaaaaat actaactatt aatttaaata
     4441 tagactgtga gtagattatt caaacaattt tactcacagt ctttttatgg catctacaga
     4501 tacaaaatta aggtctattc ctctctcccc actcacacat tgcatctaaa attggaatca
     4561 gagattttcc cctttctgat aaactatatt ctaccttagg aggtatttga ggatattcag
     4621 tacgaataat caagttatct gcttctaatt cttttaatga taaactcaaa gttttatagg
     4681 aaatagtatt tatataacgt tttaatgcat tatatctgac aacttcaaat tctacaagac
     4741 agtaaagaat tatcatttta tatttaccac taatcagtga aagtgtataa ctaaatcctg
     4801 tttcacttaa atctgtattt gatatacagt ctatactcat atattacact ctccttttgg
     4861 ttagtattga acaaaaatgt gcgtacttgt ttttagaata atttataata aaattatagt
     4921 gtaagaataa ttgaaagtca attttataat aggaggtgta tttactaaaa aaagagttat
     4981 tttaaatagg attctaagat tagacatttc tatataaatt gataatattc atgaaaatcc
     5041 tattttataa aaaataatat ctcttagaaa atctattaaa taaaattcat tggaggaaat
     5101 ttataatgaa agttttattg ataaacggta gcccaaacca atatggatgt acttatacag
     5161 cactaaatga gattactaaa gtattgagta aacacgatat taaaacagaa atactgtatt
     5221 taggtaaaga agcaatccca ggatgtatta gttgcgcatc ctgttttgaa actggaaaat
     5281 gtattcgaaa tgataaagtt aatgaattga ttgaagatct cgataatatt gatggtatta
     5341 ttattggttc tcctgtttac ttttctagtg caactggtca attaacttct tttcttgacc
     5401 gtctattttt catagctgga agtcgcatgg ctacaaaatt aggtgcttca gttgtgtcat
     5461 gtagacgtgg tggtgcaagt gcaacatttg accaactaaa taaatatttt tcaatcagca
     5521 atatgcctat tgtatcatca cagtattgga atcaggtaca tggctttact ccagaggatg
     5581 ttatgaaaga tgaagaagga ttacagacta tgcgaactct aggagaaaac atggcatggc
     5641 ttcttaagtg tatatcagca ggtaaaaaag ctggaattaa agaaccacaa tatgaagaaa
     5701 caattatgac taattttata aaataaataa aaaatgcact aatattaatt aaaacaaaac
     5761 actaatttat aaataaaata agtataaata taggataaaa aacactaaaa cagaggtatt
     5821 gaattttata tcaaacctct attttattat tacattaata accaaaccat atataacatc
     5881 atatatttct aaataaatta taatattata ttctaatgtt attgaaatta caataactga
     5941 atttctgctc ataaatttaa atattctaca aatctcatct aaataacaat tttaatagac
     6001 ctaaaacaat cctcatattg ttcattttgt attaaaaata tcacttacaa tcaaaaacca
     6061 tttattaagt atattcctaa gatatacaat atatatacat ggattggata gtataaataa
     6121 aataaatact tcatactctt ccctttttct ccattgtaaa gtaacattaa aggtagagaa
     6181 gcaatcatca tccattggta attttgtaaa atagaatttt ttaaactata atctccattt
     6241 gacattgttg taaatataaa tatacacatc aaaacataac aaatactcaa actgaacttt
     6301 ttacctctac acaaataaaa aataatccca agtataatcc aaattggtcc accttctact
     6361 aaaaatggta ctggtaccaa aatcataaat aaagtaataa ctctgttagt gactttaaaa
     6421 agtaaagcta ctgtaaatat tcctaaaatt ataggaatga ttatcatcaa taatcctata
     6481 ataaaatatt taatttcttt ctcttttcta aatttcttta tgaactctat tgcttgtaaa
     6541 taaatcgcta ttaaaaataa tgttgaaaaa atgttattta taattatagc tccttctgga
     6601 tgtgaaaata cattattaat tatttgattt cctatagcca taataacaga ccaaatatac
     6661 aatctactta tatatttttt tctgttacta gtgtgataaa atccttcaac agccatgaat
     6721 ataaacagtg gtgctgatat tctgcctacc caatgaaacc aaattggtat ttgcattgtt
     6781 tgtggcataa atgctgctat atggtcaaat gtcataaata ttaatgctaa aatcttaata
     6841 gtaaacccac ttataccttt tttaaaaaaa tccatatcat atctcctaat tttgttttat
     6901 ctctataata atttttgttc ctattccaat ttcactactt acataaattt tacctttatg
     6961 aatctctatt atttctttag aaatagccat accaagacct gaacctttat gcatctcacc
     7021 tgtattagta cctctataat attttttaaa aatatgtttc aaatcacttt tgcttattcc
     7081 aattccatta tcttttatta taatttctat attatctttt tttacaattt ctacacttat
     7141 caaagttcct tcgctattat gaactattga attaaatata agattggtta tagcccttct
     7201 gaataagatt gaatctacat atacttcaat taaatcttca ttactttcaa attcaatatt
     7261 cctattttta tatctattat catttaaaat atcaattatt atatctctga ctaaactgac
     7321 taaatttatt tttttcttgt ccagaactat agtattattt tttaatcgag tagataaatt
     7381 taaatcatca actaaatctt ttatatactt agacttatta tatataattt ctgtatactc
     7441 tcgtatttca tcttcttcaa actcataatc tttgtcatta attatctctg catacccttg
     7501 tatagatgct agaggtgtct ttatatcatg tgttatattt gccagccatt cctctctcaa
     7561 ctcttcattt tcttttctct caacttcatt tactcttaaa gtatcagcta acatatttat
     7621 atttttaaaa acttcttcat atatacctct gtccttaaga tataaactat aatttccatt
     7681 agccatagtc tcaatactcc atagtacgct tgaaattggt tttgtaagtt ttctactaaa
     7741 taaatatcct acacctaaag ctaccacgct atcaattact agtgttataa tgataacttt
     7801 atttaaagtt tttttaatgt tattttgaga ataggttaaa attattcttg atatatctct
     7861 actaggtata cctactatta tgtttagatg ttttttgttt aaatattttt ctagtacaaa
     7921 attaacatac tttgtttcta tatacttgta attattcacc atttgaaagg gtgtatattt
     7981 ttttggagta tcctttggca catttactcc ataaacttcc tctcctaaat catttaaaac
     8041 ctgtatccag gaatttgatt tttctaattt ttcagctcca atcttagata gtttaaattc
     8101 tccatctttt tcatataaat ctttttcaaa agaacgtgca aattcttcag gattatttcc
     8161 agaatctaca actttaaaaa agctattagt tgaaatcaca tacaatatag atataatatt
     8221 tataattact actgatatag ctacaaacac aattgtaaat ataaaatttc tagtaatctt
     8281 ccacttcata ttctaatctt cctttactgc taatttatat ccaagacctt ttattgtcag
     8341 aatatattca ggctttgaag gattatcctc taatttttcc ctaatttttc ttatatgaac
     8401 cattatagta ttgtcaaatc caacataatc ttctccccac actttatccc atagtttttc
     8461 tttacttata atttgatttt gattatgtgc aaataaagat aggattttta gttcctttgc
     8521 agacagtcct atagattttc catcttttat aagttctgcc ctactttcat ttaatatata
     8581 tgggccaaat gatataattt tttcttcatt cttatttaga tttttacttt cgttttgaac
     8641 atcactaaaa taacttaatc ttctcaaatg cgctttaact ctaaaagcca cttccttagg
     8701 gctaaaaggt tttgtaacat aatcatctcc tccaatcgct agtccaagta acttatctaa
     8761 ttcttcggtt ttagctgata aaaatattat tggagaattg gaggtttttc ttatttcttt
     8821 acaaatatca taaccttcgc catctggtaa cataatatcc aatacaatca aatcaggatt
     8881 aatactttta aataattcta atccttcttt tttagttttt gcagtatata tattattaag
     8941 gccctctttt tttaggacag tttctaaaag cttcaatatt tctacttcat catctattat
     9001 taaaacacga ttttccataa acaacctcct gtaataattt acatatatat tagcatatat
     9061 tttatctaaa attcatttat cttattttta attaaactaa aatataaata aaattactat
     9121 tatttttagt taaaaccttt tgcttgatgt aaaattttca tatgttattt tagttaacac
     9181 tatcattaat ataattgaga ttactataga aaaaactatc aaaatacttg gtacattata
     9241 cctaaccatt tgttggctca tcataatcca atttggagtc actgacataa attttatagc
     9301 tttatctggt ataaaaccta cactaaagta tggaattgcc attatcaatc cacctagtat
     9361 aaatggaatt acagccgatt taaatataga tgaaagtgtt aagcctattt gtgcaaatat
     9421 aaaagaagca aaaatagtag tcaatataac aattatatac atttgtatta ctgtaagatt
     9481 taaattacta tttccaaagt accataagtt atgaatcggt tcatttaaat ttttaaaatc
     9541 aaaaaaacta aaacctctaa ttcctactaa caatataaaa gaaatattaa taatagataa
     9601 aattaatcca ttagctacta gttttgataa tactactgat ttttttccct ttttagttgt
     9661 atttattaaa cctgtcatat tagaatcata ttctttaatg taatttccag aagttattat
     9721 aaacattatc aaacctatta gaggtattga gccttctatc ttgtaaaata tatctgcata
     9781 agtatctaaa acatcaaact ttctaatcag aagaaatccc aaaaatatta aaaacagcca
     9841 aattatccaa acactctttg attttaatat ttttatcatt tcagctttaa ctaaatacat
     9901 catttctact acacctccct tgcatcttcc aaatcaaaat aaaacatgta tacatcttcg
     9961 aattttgctt gaactggttg tatattatcc ccacttggaa tatcctctga aacaattcta
    10021 agtactactg aatcaatatc actttgaata cttacaacct tatatttaga ttgtatatca
    10081 tcaacttcac attcattttt aacttgaaca ttatatactt tattttccat gtgacttaat
    10141 atgtctttgt gtgttccttt cattagaagt ttgccatctt taatcattat tgtttccttt
    10201 gcaactgatt ctatatcgga aattatatga gtagaaagta ttattatctt atctctagac
    10261 atttttgcta ttaaattcct aaatctagct ctttcttgtg ggtcaatacc tgctgttggt
    10321 tcatcaaaga ctattatctt agggtcgttt agtaaaactt gtgctatccc aactctcctc
    10381 ttcatacctc ctgaaaattt tcctatagac ttatttctca catcgtaaag acctactaaa
    10441 tctaataatt ctcctatctt actatttaaa tattttcccc ttaaaccttt taaagctcca
    10501 atatactgta aaaattcttt tgctgtaaaa tttttataaa ctccaaattc ttgtggcaaa
    10561 tatcctaaaa tatttctata attagcatcc attttaaata tatcctcacc attataaagt
    10621 atctgacctt catcaggttt tatcaacgtt gttatttgtt tcattaatgt agattttcct
    10681 gctccatttg gtcctagaag accataaact ccattttcca gagttactgt aatcttgtcg
    10741 tttgctactt ttttaccata tatctttgaa agattttcta tttctaattt attcatctta
    10801 actccccttt tatctactta tataatctga ttttattact aaaaaacata atgacatagc
    10861 acctatcaat attgccatca aaagtcctat tgctatattg acaatgtcta tattcacttt
    10921 aaatatcata atatcaccta gataatttcc tattatttta ttaaaaccaa atatcaaatc
    10981 ataataattt acatttgata caactgaaag taatttttta ggtaatatat ggattaccga
    11041 cattatctta gttatagata taataaatat aaaaaaactt attgaagata ttgaagattt
    11101 acttataaaa gacccaaata tggctatcat ggcccatcct tcaaacatca aaagctctat
    11161 gcctatattt aatattacat actttgttat acttaaatta gttttcaatg caactacatt
    11221 gtctactatt ctaaaagatt ctaagtctcc attaacaggt actccttgtt gcatacaagt
    11281 tataataaat attcccatta aataaacaga ataaattact aatggaacta ataaagcaac
    11341 aaatatttta gactttaatg cttttttctt ctcttttgat gatagtatga tatcttttac
    11401 cgaagtaacg acttcatcta cgtaaatatt ggatataatt atagatataa tgattaacat
    11461 tcctacattt ataagtgaat ttgtaactct atagttaaat actttccaaa aactaatatc
    11521 cttatattca ttcacactta gattatcaag ttttttcttt gacattaatt caattttttt
    11581 agaaaattca cctgtatctg tttgatttga aaactgcttt aaaacattaa tcttattttg
    11641 aaatttctca ttacctatgt cgataccact tctcgtatct tctatacatc ctttttcttt
    11701 atctatataa taacgttctg tttctaaaac aggcgtaata aagatactaa tcattagtat
    11761 taacactaat atccctagag atataaacat actcttagat ttagccaatt tttttatttc
    11821 ccaatttacc aatatatttc tcctcctaaa tcattttaat tacacatata tattaaaact
    11881 taaatcttat ataaatataa tactaacctt aaataaagct taaattattt tataatttat
    11941 aggtgtgttt attattggag ttacatttat tatttttctg ggaaattata cattttcaat
    12001 tatttttatt atttttgata tttttattga ttggaaattt tattgttgtt caaattgtag
    12061 ttattcttta aatttaaaga tggagttttt taagaataga acctactgtc cttattatgg
    12121 aaagattatt aaatagttgc atataacttt atttatcttg aatcttattt ttaaaaacaa
    12181 ttataatttt agaatatttt ctattctcaa acaagcatgt tgaataatga agcatcttct
    12241 agtattatat acttgtagaa taaaactaaa ttggtaaaac tgggaatatt taaccttaag
    12301 gagttaagtt atagaaacta gtactaaaaa attttataaa ttatgttaat tgactaatag
    12361 aatgcgactt tttattttta ctaaattttt tagggggatg gataatatgt ttattggaga
    12421 aatagacaaa tgtacacata tactaactgc ttacatcagt agttcatatg attattgtaa
    12481 ttttttagat acacagttag atgattttat atcagaatat ggagaaacgg tagttgaaac
    12541 ctgcttacat caagtcttat tattagtaag tagatacaat taaaattaaa tttttctaga
    12601 aaaacctttt tagatttatc tactctaata aatatataat tttttgtact ttataagtaa
    12661 ctataacttc tgctaaagta aagatgtatt gggagtacat ctttactttc ccattataga
    12721 agttaaaagc atacaccata aaatcaatca tccaatacaa attatatatt tcctatgtct
    12781 aacttaattt acaagaatca ttctatatgc atattctgca atccactaaa catgctctaa
    12841 acctttacta ttttatacat tctctataaa tcatacaaat catatataaa atccccctaa
    12901 tatgtttaaa ccacctataa aataacacca atatttagaa tattcaatat caactgcata
    12961 actggtcaat aaaaacgtat aactagacaa atttcaatat ttatataaat aatacaagaa
    13021 aaacaagcat tttatttata taaattt
//
LOCUS       DAMKPG010000098        12871 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMKPG010000098 DAMKPG010000000
VERSION     DAMKPG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.103"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            131..319
                     /locus_tag="PC621_002992"
     CDS             131..319
                     /locus_tag="PC621_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF4110133.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(479..631)
                     /locus_tag="PC621_002993"
     CDS             complement(479..631)
                     /locus_tag="PC621_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF4110134.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(729..1205)
                     /locus_tag="PC621_002994"
     CDS             complement(729..1205)
                     /locus_tag="PC621_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF4110135.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1424..2185
                     /locus_tag="PC621_002995"
     CDS             1424..2185
                     /locus_tag="PC621_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110136.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2445..4373)
                     /locus_tag="PC621_002996"
     CDS             complement(2445..4373)
                     /locus_tag="PC621_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF4110137.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4607..5059
                     /locus_tag="PC621_002997"
     CDS             4607..5059
                     /locus_tag="PC621_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4110138.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5062..5370
                     /locus_tag="PC621_002998"
     CDS             5062..5370
                     /locus_tag="PC621_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF4110139.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5484..6590
                     /locus_tag="PC621_002999"
     CDS             5484..6590
                     /locus_tag="PC621_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF4110140.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6665..9343
                     /gene="mngB"
                     /locus_tag="PC621_003000"
     CDS             6665..9343
                     /gene="mngB"
                     /locus_tag="PC621_003000"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF4110141.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9660..11993)
                     /locus_tag="PC621_003001"
     CDS             complement(9660..11993)
                     /locus_tag="PC621_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4110142.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11968..12684)
                     /locus_tag="PC621_003002"
     CDS             complement(11968..12684)
                     /locus_tag="PC621_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110143.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4804 a         1790 c         1663 g         4614 t
ORIGIN      
        1 tatgataata agaaattctt caatatgtgt aaggcaaata tatatttcta ttgcaaattt
       61 ttgttattta tgataatata aatatgtttt tagtctttaa atagttatca ttttaaggag
      121 gtgtgcgtag atggcaaaag tatgtagcgt atgtgataaa ggtaaggttt ctggaaacca
      181 agtatcacac tcaaataaac ataataaaag aacatggtca gctaacttaa gaagcgtaag
      241 agcgattata gatggagctc ctaagagagt aaaagtttgt actagatgtt tacgttctgg
      301 taaaattgaa agagcttagt aaccacgttt aaaagcctac ttccgtaagt aggcttttta
      361 atttaatcat ttttactctt tctcataaat tttaggatta tacttgaaat ccattttggt
      421 aattttattg ttactacttt catcaatact caccccacac tttatagtta aaactatttt
      481 aacaaaaaaa taataatgct ccacatccaa taagtattaa tgcggtaagt gctgaccaaa
      541 tccagccagg aagcaacatt gacagaatta cagccattcc tgttgctaga caaagtattc
      601 ctagcaatac cttagacttg ttatttctca tggaacacct ccacatagaa cctgtatcta
      661 taaatatttt catattcaag tttatgcgac agttttccat tttagaccaa taaacacatt
      721 atgtaatttt aatttttcat actagaatta ttactgaact ccgatataga ttctacaata
      781 tccaaaattc ctttttttct acttaacctt tcaggtccac tatataaaac cactggtata
      841 actttaggaa gtttaaaatt tttatcttct atttcttcaa ggcttttttt cataataaaa
      901 cttttccaaa tttgttctct atacgatttc aatcttggga caatttcagt atccatttct
      961 gtttgaaatt ctaacactat ataatgtgat actttgccac ttttagaaaa taccttataa
     1021 attatatcaa cttcttttcc tttatattca catataaaag atgtttctaa ctctatattt
     1081 ttaagagtta attcttttgc tattgagaca cttgtaaaat tttgaatcaa acttaaaaaa
     1141 ctttctttat ctgaatacat tcttctgtac tcttctcgtt tttcattgct tctttttaaa
     1201 ttcatacagt tcctcctagg ctatagacct tctatatttt agtaaagata gtctataaat
     1261 tttatttttt aattttcatt agtgagtatt gacagttgtg tgtaaaatat ccagtaaaga
     1321 tattttttta tatttttatt aaaagatata aatttttata ttttaatata ttgacattta
     1381 cgttgcgtaa aagtgtatat ttaatttata ggaggtatta cttatggaat atacagttca
     1441 aaaattaagt aaactagctg gtataagtac tagaacactt agatattatg atgaaatagg
     1501 tcttcttaag ccacttaaaa taaattcttc aggttatcgt atatatggtc aaaatgaagt
     1561 taataaactt caacaaatac ttttttatag agaattagga attaacttag aaaatataaa
     1621 aaacattata aattcaccaa acttcgatag tttaagtgcg ttaaaagaac accataataa
     1681 attactagcc aaaagaaagc aaattgattt actaatagaa aatgtaacta aaactatagc
     1741 tctgaaggag ggaaaataca ctatgacaga tatagaaaaa tttgaaggtt ttaaagaaaa
     1801 gatgattgat gaaaatgaaa aaaattatgg aactgaaatt agagagaaat atggtaaaga
     1861 tattatagat acatcaaata aaaagcttaa aaatatgtct aagcaagatt atgaaaattg
     1921 gcaaaaccta aatatcgaaa taattagtaa acttaaaaaa gcttttaaaa ctggtgatgc
     1981 ctctagtgaa ctggctcagg aagttgcaaa attacatcat aaatggttga gttacacttg
     2041 gaacacatac tctaaagaag ctcattcctc tctagcacag atgtatgtat atgatgaacg
     2101 ttttacatca tattatgata gagaacaacc tggattagct aagtttttaa gggatgctat
     2161 agtaatatat acaagagata aataatataa aataataaag gtggtgtctc aaaatgaact
     2221 ttttagttca tgagacactc ttttttgtat gaaatcaaat atatttttat cattttatca
     2281 atgaaaaaca agtttctctc tctattttga gacagaatct ttattgtata ataattataa
     2341 tcaatacaaa ataataacaa attatacaaa acttacttat actatttatg ctaaaaacaa
     2401 aaaatatttt attaaagttt ttaaaactta tgtaatataa aaatctatac tatagacttg
     2461 attatggaaa gaaaattatc aaaatcatta caattagata tactatctct aatatcattg
     2521 tcatgaatta tctttaaaag gatatcataa atggagttta tttttatata atcatcttta
     2581 tttatagcta acatcatgat taatttaaca gtagaatctt cactccaata tattggttca
     2641 ttcaatatgg aaatacaaat cttagttctt atagagctta attccatagg atgaggtata
     2701 gctaaaaaat catctatatt ggtagaagat aaactttctc tgtaaaatac agattcagca
     2761 aatgatggaa atacaatttc actttgagtt aatttgttgc acatcaactt tataagctca
     2821 tctttatctt tgattttagg attaacaata aataaatttt tatcaaagaa attgtccaaa
     2881 agattactat aaatatggga attattagtg attgacttag atatattctc tatatctttt
     2941 tttaatagtt ttaaatccac taatacaata ggaatactat catgatttaa aggaattgtc
     3001 gatattatta tatctacatc tgacaattca ctagctaaaa attgattaaa tgatagacat
     3061 tgaagtatat ttattttatc atgaaaaact ttgtttaatt gaacctctaa taacctactt
     3121 gaaccatacc ctgaaccaca tactaatact acgtttttac acttaatatt attttgaaaa
     3181 aatctctcta ttccagcacc tatatgcagg gatacatatc caatttcatc ttctgttaaa
     3241 gagtaaatac tatttttaaa aactttttct atagcattta aagttatttc aaatgctaaa
     3301 ggataattac tctttattgt atttataagg ggattcacct tatttaaatt ataagattta
     3361 gaattgagaa ttgatttaaa gtgcagtact aaatcatcaa ataatacact atcatttcta
     3421 aggtcaaaag tatattgatt gttgattact tctaaaattt cttcaacata ctctataatt
     3481 ttagtatcca ccctattgga aacgttatcc aaaagagaac ttttagttat aaaatgatta
     3541 tatagataaa ctctgtctgc ctttgaaata attatagtat atttactctc aatataatta
     3601 aatatatttt caacagttgt attcgattga aaatctgtta aaacaacatc taaaacatta
     3661 tctaagcagt aaccatcaag tattctactt atagtaatag ctaaatggat aataaagttt
     3721 tttagattaa aatctagaaa atttatatta aatttattaa tttcacttaa cactaccctt
     3781 tgaagctcta ttaaatctac attattaaat aattctattt ctctatctgt aattccaatt
     3841 atatagtttt caaaattcct atttatcata ttattagaaa tacatcttcg aatatctatt
     3901 tccttccctt caactttaac tccataatat ggcttagaaa ctaatattaa attatacttt
     3961 gacaaaatag gctttattct ttttatgtca tttagtattg taaatttact cacatacaag
     4021 ctattagcta aatcttctag ttttatatat gtattgttat agagaatata ttttaaaata
     4081 tattgatttc tttctatagg agaatctggt atttcacttg aatccataat atcatcagat
     4141 attaatgcta gatactgttg gtataatgct aaattaagaa tatctatata atatccttct
     4201 ccttttttta ttttaataat agcaccattt ttctctaaag ttccatttat agaagtaata
     4261 tcatttctta tagttctctc actcactttt aaatgattac ttaaagtttt acaagtaatt
     4321 ttatcatcac tttcatttaa taatttaaac aacgtgagca atctcttgtg cataaatatc
     4381 cccccttata gaatttcttc taaattatac tatttacact catataatac aattactcta
     4441 ataaaattgt attataataa tttctttcca cttctatatg gaaattagtt attatcataa
     4501 tataaatttc catgtcgaag tggaaattag ttatatttaa tttaattagg ataatgatta
     4561 caatataaat ataagttaag acattacgaa tggagtggaa aaatatatgg tactacaaga
     4621 aaaatatata tttatagata aagacttaaa aagcaaagaa gaagtattaa attttattgc
     4681 agatagtgct tatatactag gtattgcaac taataaagag gctcttttaa aagaccttct
     4741 aaaaagagaa aatgaatttc caacaggaat aaaagatgga tttgcgatac ctcatacaaa
     4801 aagtgtaaat gtaaaggaag cttccatact ctatattaaa acaactaacg agttagagtg
     4861 gaaaacttta gatgatagta aggttagata tgtatttagt ttgttagccc cacataaaaa
     4921 tgaaggaaat atacatttaa aaactctatc taaattagcc acatgtttaa tggaggatga
     4981 ttttacaagt gaagttaaaa atgctagtga tgcaagtcat ttagtaaaat atataactaa
     5041 taaaatgaag gaggaataat tatgaaaata gtagcaataa catcatgtat agcaggatta
     5101 gcacacacac ctatggcagc aaaagcttta gaacaagctg catcaaaaat gggtcatgaa
     5161 atcaaagtag aacaacaagg tgctatggga cgagtaaata ctataacaaa agaagaagca
     5221 caagatgcgg actttatact tatagcatct gaccaaacta tatctggaat ggaaagattt
     5281 gatggtaaaa aagtgataaa agtcaaaata ggtatagcgt taaaaaaacc agaagctgtc
     5341 ttaacaaagt gtatagaagc tatttcttaa taaaaaaatc tcactctaat atagaatgag
     5401 aaaaataaaa aattaaaaaa ttgtctaatg taattataac atattatcaa aaaatataac
     5461 aaacttatat aggaggatta aaaatgagta aaaaaaataa atctctttta agtgaactta
     5521 agggacactt aatgacaggt atctcatata tgttgccact tgtaataggc tcatctttag
     5581 ttgtagctat cccaaagctt acagctttag caatgggtat aacaagttta gatgcttata
     5641 aaaatgtatc tggtttttat catattcttt acttaatgga acaagttggt tggactggca
     5701 taggtctatt aaatacggta ttagcaggat ttatagcatt ttcaataggt gagaaaccag
     5761 ctatgggtgc aggttttatt ggtgggctca tagcaagtaa tactaatgca ggattcttag
     5821 gagcagtaat agcaggtttc tttgctgggt atgtttgtaa gtttttaaag aaaaaaatta
     5881 aaatatctgg gtcagcatca ggaatgatgc cacttataat aatgccactt ataacagttg
     5941 gattaactgg ttttttaatg agtgttgtat tagcaggacc tttaggaaac ataaatactt
     6001 cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac aaattcggtg gtattggctt
     6061 taattttagg cgcaatgata ggattcgatt taggtggacc agtaaataaa gcagcttgga
     6121 tggcaggaaa tgcattattg ttagagggaa tctatctacc agcgatattg gtaaactgcg
     6181 caatagttat acctccacta ggatatggtt tagctacatt cataagaaaa tcaaaatttt
     6241 ccaatgcatt agctgaaaca ggaaaaggca atatagttat gggtataatt ggaataacag
     6301 aaggtgcaat tcctttcact ttaacaaatc cattaaaatt agttcctgtt aatatgatag
     6361 gatgcgcttt gggtgtagga ttatcagctt tgttaggtgt acatgctata atgcctccag
     6421 ttggtggatt atatggattt atatcaatag gttctggatg ggcttactta gtaggtgcaa
     6481 tatttggagc tttagtaata gcaatattat caacaacttt agtagacttc aatgaagatg
     6541 aaactacgga aaatacagaa tttgatgaaa tagagttaga tataatgtaa attaaaaact
     6601 taattttaga taattgagaa ttgagtgtac tctcaattct caattaaatt ttgggaggat
     6661 ttttatgaaa aagacaaaag tacatgtagt tccacacagt cattgggata gagaatggta
     6721 ttttacaaca tcaaggtcaa aaatatattt aatgaaagat tttcaagata tattagacat
     6781 attggaagaa aaagaggact ttaaatactt tactatggat gcacaagctt ctctcttaga
     6841 tgattattta aaatggagac ctcaggatga atatagaata agagatttag ttcaaagaag
     6901 aagattagtt attggacctt ggtatactca aacagaccaa ttggttatat caggagaatc
     6961 tataattaga aacctttact atggaataga tagatgcaat gaatttggtg aacctatgag
     7021 ggttggatat gtaccagatt cttttgggca atcagctcaa atgccacaaa tatacagagg
     7081 ttttggaata gatagttcat tattttggcg tggagtttct gatgatatgg tagatacaac
     7141 agagtttata tggaaaggtt ctgatggttc aaaagtatta gcagttcaaa taccatttgg
     7201 ttattactat ggaggtaata taccagaaga tgattcagat ttaaaagaat atttaaaaga
     7261 tataattgga aaacttaagg ttaaagcttc tactaataac gtatattttc caaatggatt
     7321 tgaccaagca ccaataagaa aaaaccttcc tgatatatta aaaaaagcta atgaaataga
     7381 taatgaaaat gaatacgaaa taagtaatat agaaaactat atagattcag taaaaaatga
     7441 aagaaatgaa tttacagagc ttaaaggtga atttttaaat gctaaacata tgagaataca
     7501 caaatccata ttctcctcaa ggtcagacct taaaataatg aataacaaat tagaaaatta
     7561 tatcacaaat gtacttgaac cattattaac attaagttat tcacttggaa atgaataccc
     7621 tcatctagtt ataaaagata tatggaaact tatgtttgaa aatgcagctc atgacagcat
     7681 aggttcttgt aatagtgaca cagcaaatga ggatgtatac acaagataca aacaagccag
     7741 agatttgagt ataaatttat tagatttgca tatgagactt ataagtactc aaatcaaaaa
     7801 taataatgaa gaaataacat taacagtatt caatggattc ccagaagtca gaaatgaagt
     7861 tgtagaattt gacacttata ttccaggtca agattttgtg ataaagaata aaaatggaga
     7921 aattcttaat tatataataa aagaaaaaga agatattaca aattatatat taactcaaac
     7981 aataagatta aacccaagta aaaaaatata tatacccaaa actgtatata gagcaaaaat
     8041 tgcactttca gtaaaagata taccaccaat gggatatact caactagttt ttgaattgga
     8101 tactaaaaat aaatgtgaaa tagaaacaag taattgcaaa gaaatagaaa acaaatatta
     8161 caaaataaca gccaataaaa atggttcatt agaaatatta gataaagaat ctagtaagac
     8221 ttataaaaac caagctgtaa ttgaagacaa tggagatgat ggagattcat acaactattc
     8281 tcctccaaga aaagatattt atgtttcatc tttagattct gtatcttcag tagaagtgct
     8341 aaaatcaaat attcaagaag aaatgatact taaatattca ttgaccatac cagcttcact
     8401 ggaagaaagg tctataggta aagtttctgt gaaaatgcca gtaacaatga aaattacctt
     8461 agaacctaaa gaacaaataa ttaagtttaa tgttaatata aaaaataacc aagcactatc
     8521 tcatagagta agagttttat ttaatacaga aatagcatct aagttttcta tagcagatca
     8581 acaatttgga ataatacaaa gaccaacagt attagaaaaa gaccttgcat tatggaaaag
     8641 agacaattac tcatggcaag aaaaacctat aactatcgaa ccaatgcaaa gttttgttac
     8701 tttagaggat ggacaaagag gaatagcatt aataactggt tgtgtaagag aatatcaaat
     8761 agttggtgat aatttagata caatagcatt aacactattc aggtcatttg gatatatggg
     8821 aagagaaaat ttactttata gaccaggaag agcttctgga gaaaaaataa tagcaacacc
     8881 agatgcacaa ttacttaaag agttaaactt tgactttggt ctttacatat ataacaataa
     8941 atttgatgaa gcaaatgtag ctaatacagc taagagattc ttaacaccta tacaaatata
     9001 tgaatatgct gattttttaa atggtagact tctatttgca ttcaatgacg aaaaacaaat
     9061 atatgaaaat gaatttagtt tgatgaatgt aaataattca aatttcacag taagtgcaat
     9121 aaagaaagaa gaaaaaggag atggaattgt agttagaatc ttcaatggta tgaaaaatga
     9181 ggatgctaag ggaagtctta acatcaataa aaatgtcaat gatgcttata gtgcaatgct
     9241 cgacgaaatc tatgataata atagcaagct taaagtaaat actaaaactg tagaggtaaa
     9301 ttctttatca cattgtaaag tacaaacaat agtaattaaa taatataaaa tagcttatac
     9361 aagatttcaa aataaattgc tagtactatg ttaatagtaa acccagatat tatgctattt
     9421 ataagctctg taacactaaa taacattttg taaaataaaa aggtgaacct tgtatattag
     9481 gattcacctt ttttaaataa atcaacattc tgttttaaaa taacatttat aagctgtttt
     9541 gaaataatac ttacaaatta ttttaaaaca atacctataa atttttattt aaagaataca
     9601 cataaatatt tccagactct tttattctat agactttata attattaaag aagtctaatc
     9661 taatttttgt taaaagtaat tgtaacttta aataaatcac catccacact tatattcatc
     9721 ttaccaccct gtaattctat aaaacttttt gctattgcta aaccaagacc agaaccttct
     9781 gtgttacgtg atttatctcc tcttacaaat ctctccatta tatcatctac actaaaatca
     9841 atctcagatg cagacatatt tttaagcata atatttactt tattatctgt ttctaaaata
     9901 tcaatataca ctcttgaatt tggcattgca tatttgctta tatttattat aagattttca
     9961 aatactctaa atgtacgctg actatctaat ggtaatatta ttttttcatt agaaaagtta
    10021 ttctttacta ttagagatga atctgcaagt ttgtcatcta attctaaaag tgtctgcttc
    10081 attagagata caatatccac atttactata ttcaaataaa catctccact atttgccttg
    10141 cttacttcaa acaaatcttc tattagatgt tgcaatcttt gtgactttct gtctaatgta
    10201 tcaatataca acttacgatt ttcatctgtt atactttcat cttttaataa atctatataa
    10261 gtgattatag atgttagagg tgtttttaag tcatgtgata catttgatat aagctctgtt
    10321 ttcatctttt gactcttaac ctcttcgtca acagcttttt taaatccagt ttgaatattt
    10381 cctaattcgt cttttatagg attaaaaaat cctaaatctt tttccatatt tacatctaag
    10441 tttccatctg aaattttctt tgtaacatct aaaagttgat tatattcata tattgtcttt
    10501 ttagagtaat ctgacagtat cttaaataat actactgaat atattaatgc gagtattatt
    10561 ccaaagaacc acatactaca cattataact ataatcaata aatttattcc caaaattgta
    10621 actatagttt tatttggctt ttcttttaga tttattgttc taatttgatt aataactgtt
    10681 tttcctgctt tttttaacca tacaaaaaac attcctgtca cagtatgttt tgtaaaatac
    10741 tctcttattc caacatttaa aatatgtttt aaaagtatta taaatacaaa tattattgag
    10801 aatattacaa accaataagt taaattcatt aaacttacca atatgctagc cattttagca
    10861 cttatgtcat tgtttataag gtactctaca taattcccac tcaaggtatt acttattata
    10921 acagtaggat ataaatctac aagcccctgt ataatagcaa gagcaataaa gagtatctct
    10981 aacggtattc ttaataatgt atcaaatcct agcaactctt tagattttct atatgggaat
    11041 gctaaagcta caataacaac tatagctaat ataattttta aaatcattcc agaaatctta
    11101 taataagaat tattttctac attgcttata tatgaaatta taccatcatc aaaataagta
    11161 tttccataac catactctac actatcacct ttacttatta ctttaggtac agcatatact
    11221 aatgttatat tttttattgg agataactta tatgaaattg aatttcctcc taaacttgta
    11281 tccattaaat cttctcttgc tttagataac aacatttgat taataacttt ttgatttact
    11341 ccataagaat ccacaatatt tacttttcca tctttatcaa aattcattac aacgtagaat
    11401 ctatagtttt cctttatatc tgtaatctgc ttttgtcttt cctcatcatt catttctgta
    11461 ttttttgact gattttcatc agaattttct tttgaagcat cattctttgt tgtatcaagt
    11521 actcctaatt ctttattatc attaaaaaat tcaaaattgc tttctttatc aactaaatag
    11581 tactttaaat tctttcctga cgctttaatg aaatgctccc aattttcaaa gttttcatta
    11641 aaatctgcat taatactatc atctgtataa taagtttctg aaccatcata agtatctttt
    11701 tctttactta cattttttat atagttcata gtttcttcat tgataaatag ctttgatggt
    11761 tgtatcatct taccttcttg ttcttccttc atattatgat ataaaacata acttccttta
    11821 tatatactat ctacaaagct ttcctgttca aatggattct ttgctccacc tttagcacct
    11881 gcttgtatag tagaatattg actaatcata cctaatgatg ctgataacat aagtataaaa
    11941 actataatag taactatata tttatcttta aattttttca attttgtatc caacacccca
    12001 caccaccttt aaatatttag gattttttgg gtcaatttct atcttttccc ttatattcct
    12061 cacatgtacc ataactgtat ctgtatttat agcattttca ttccaaactc tttcatatat
    12121 ttcatcagca gaaaaaactc ttcctgcatt tctcataagt aaacttagta ttttaaattc
    12181 tataggagtt gtttttatat attttccatc aatattaact tcttttgtat tttcatttaa
    12241 ttctaatccg cctattgcaa acacactatc atcactttct accttttgct ctttatttcc
    12301 aaccatgttt agatacttag tgtaccttct aagttgtgaa ttaactctag ccaatagttc
    12361 taatggctta aaaggttttg ttacataatc gtctgctcct atattaagcc ccattatttt
    12421 gtcaacttct tctgattttg cagatagcat tattactgga aaattatagt tttctctaag
    12481 cctcattgta agtgttatac catccatttt aggcatcatt atatcaacta tagctaaatg
    12541 tatttcttgg ttttctataa ccttaagacc ttctaatcca ttgtatccct taaaaacatt
    12601 atatccttga tttaatagat atatctctat agcatctgcg atctcctttt catcttctac
    12661 caccaaaata ttatatgaat tcattttaat ctcctttata ttaatttata aattttcttt
    12721 acatatatga ttatacaata tgatagaagt ttttccattt atatatatat ttatatcttt
    12781 tgttctggtt atttgtattt aatgtccatc ttagaaacct ccttctagtt taaacttaat
    12841 attttttcat tttgttttat atacttaata t
//
LOCUS       DAMKPG010000099        12861 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMKPG010000099 DAMKPG010000000
VERSION     DAMKPG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.104"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(147..746)
                     /locus_tag="PC621_003003"
     CDS             complement(147..746)
                     /locus_tag="PC621_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF4110144.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(764..3883)
                     /locus_tag="PC621_003004"
     CDS             complement(764..3883)
                     /locus_tag="PC621_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF4110145.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            4104..4928
                     /gene="pdaA"
                     /locus_tag="PC621_003005"
     CDS             4104..4928
                     /gene="pdaA"
                     /locus_tag="PC621_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF4110146.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            5117..5881
                     /locus_tag="PC621_003006"
     CDS             5117..5881
                     /locus_tag="PC621_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110147.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            5984..7162
                     /locus_tag="PC621_003007"
     CDS             5984..7162
                     /locus_tag="PC621_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDF4110148.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(7533..8318)
                     /locus_tag="PC621_003008"
     CDS             complement(7533..8318)
                     /locus_tag="PC621_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110149.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            8783..9148
                     /locus_tag="PC621_003009"
     CDS             8783..9148
                     /locus_tag="PC621_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110150.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            9226..9780
                     /locus_tag="PC621_003010"
     CDS             9226..9780
                     /locus_tag="PC621_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF4110151.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            10000..10674
                     /locus_tag="PC621_003011"
     CDS             10000..10674
                     /locus_tag="PC621_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF4110152.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      11029..11125
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11179..11355
                     /locus_tag="PC621_003012"
     CDS             11179..11355
                     /locus_tag="PC621_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110153.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(11543..11782)
                     /locus_tag="PC621_003013"
     CDS             complement(11543..11782)
                     /locus_tag="PC621_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110154.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(11927..12736)
                     /locus_tag="PC621_003014"
     CDS             complement(11927..12736)
                     /locus_tag="PC621_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110155.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         4466 a         2002 c         1442 g         4951 t
ORIGIN      
        1 ttcataatac aatctgcttt tttaatcaaa atttatttaa ttataagttg tttaattaca
       61 agttgtttaa ttacaagttg tttaattata aagtaggaat ttagatatac tcttatacca
      121 tgtaattatg tataaatatt tttttattat acaccaatag cttttttagc taatttatca
      181 gcttcctcat tatacttatt tccagaatga gctttaacct ttgtaaattt tacattaagc
      241 ttattcttaa tactgtcata gaaattttta tatgctatag ttccttcttt atttgttttc
      301 caaactccag tacaccactt ttcaatccct tcatagtcaa agtataaaat caagttttga
      361 acattattgt caatacagta ctgcattgca attttagaag cctctatctc tccagctaca
      421 tttctcatac ttactaaagt tttctctttt cctttttcac tataagtttt tataacttca
      481 ttatttttta aaatcacaac acccgaacca tacattttta ctgaatgttc ataacttcca
      541 tctacatatg cttctataaa ctccttatct tcttgaaaac tcaatttttc tgacccatca
      601 atatattctt tagcttcttg aaaagtagaa aaactcttat attcagctcc tgagaatcca
      661 tttacttgtt ttttgcactc atcccatgta ttatatacac ctatattttt accttttttc
      721 acagcataaa acttttgttt tgccaaatat ctcactccca ctcttaattt ttatcttctt
      781 ctctcataaa tatatctaaa tttataagat agtttctatc aaaatatttt actttcatat
      841 ctttttttat aatatcaata tggtacactt ctccttttac agctctttgt cttatatttt
      901 catttatatc tttttttata atatctttat caacatcatt gttatccatt ttagcatcaa
      961 cattcaacgc ttttatagta tcaaatataa cagattttag tattaaactt ctagatttat
     1021 ttaaatgtcg ccttatataa gtataaaact cattgctatt aacctttgaa attttacaat
     1081 cgtgtaaatc tttctctaat ctctttaaaa cacttatatt agttcctaca tcaaaaatca
     1141 caaaacatcc atagtcatat ttgcttctta aaattctccc catagcttgc tttaccttta
     1201 tggtcatttg aggataattt atagtatagt agtctatacc atacttcttc attatagtaa
     1261 aatacaaagg gtcttttgga tttagattcg gaagcttgtc taaagttaca caaaccaaac
     1321 catcccctgg aatgtctacg ccttcaaaac atccttttga gccaagtact acacattttc
     1381 tatttaaatc gttaaggtgt ctaatcccct ttttatcagc ataaatttct aagttaaaac
     1441 tatgtaagta ttttttcaat atttcataag ttttttcttg tctatcttta ctattaaaaa
     1501 gagctaatgt atgaccttct gtaatctttg atatattaga tataatttta ctcatctcat
     1561 ttggaaattc agaatttctg tactcacaaa tatcagaaaa tccaactaca gagactctat
     1621 ttttataatc aaaaattggt tctataatct tttccacatt cttaactctg tctattccta
     1681 atgtattttt aaaataagac atactttcag ataaactcaa agttgctgat aaaaatattc
     1741 ctttttctaa ttgagataaa atattttctt caaataaatc tgctattttt aatggtacta
     1801 ctcgaaattc aaagtcatta taattcttat caatttcaac tatccttgca taatcatcct
     1861 tttcatcata ctctaaaaag atttcaaata taatctttat atcctctaaa tctcttgtct
     1921 ttgcctttcc aaatttatat atatctgctt ctttatctat actatcatca tcaatattcc
     1981 tataaattat tattattatt gaaactaaat tttttattat tttctcacaa ctcaacttta
     2041 tcttttcact ataggctcta taacttattt cagtatctat accatccttt tttagttttc
     2101 ccacaatttc atctatttgc aaattcagtt cccatcttaa attataatta cttacattat
     2161 tatattcatt acaattacca aactttaata ttgaatctat ctcttcaata atgagattta
     2221 tacttctagc aattttttgt ttatcttctc ttccaatttt taatacatta taaaatttat
     2281 caaaagcctt tatttttctc atatttctac tatatttttt atatataaag ctactatttt
     2341 gaataaactc gtaaggataa atttcttgta ataaatacct taaagacttt gaatttataa
     2401 tgcttgaaaa gaaatcatat cctttttcag tcaaattatg tgcttcatct actattatat
     2461 tttctaaagg tttttcatcc ttatacggcc atttagctaa tagtgaatga tttactactg
     2521 ttatatgttc ttcttttaat tcttctattc tgtttttata taaacaatct tttttgcatt
     2581 ttttaggtct gcacatattt gggtcacaac ttacatttct taagtgggta cttatttcct
     2641 tgaagttgtc aaacactaaa taatttattt cttctatatc tccatacttg ccaccttcaa
     2701 ctaatctctt caaaaatatt aaagataaaa tttctccttt agtagggttt tgagactcat
     2761 aatcatctat atatgcttcc agtctatcta tacaaatata gttatttttt ccctttatat
     2821 atccgtaact tacttttcca tttagaccca aagaatctaa cacatttgga atatctttat
     2881 ttattaattg tatctgcaat tcctttgtgt cagtagaaat taataatctt tttttattaa
     2941 ttctagcctc taatattgca ggcaataaat atcctacact tttacctata ccagttggtg
     3001 cctctataca tgcaattttt tcttcatctt cgctatttct aaacaactct cttattgttt
     3061 tagtaagctc atattgacca ggtctatatt catatgtaaa tccttcttta ttctcccaaa
     3121 tagttttgta ttttaataat tcttcataat ttttttcttc ttcatttaaa atttttaagg
     3181 tttctctttc cttcttcttt tcttcttttg agtcaaatat attatattct ttttttatat
     3241 ttatattatt tgataaatca taatttgcct cttctaaaaa tttgctccat tcccatgttg
     3301 gaagattaaa ttttttaaga tatgaattta ttttaaaact caatggctcc aaagtagttt
     3361 tttcatagtt attaaatttt actaaaaggc aatttacaat atttattata tcaatagcat
     3421 ctgataaagc tctattttcc actttagaat tacaatttgt taaagtattt tttaagtatt
     3481 ctaagctata atctttatga tatggttcta aaataactgc taattcaatt aaatcaataa
     3541 atttgttttt taactttggc atatgatact caaaaaactt ttttttcaga cttaaatcat
     3601 gacatataat gaaactatct tctacaaaat ttacaagtct atcttccacc agttctattt
     3661 cattagctat ttctaaatca tttttatcta aatttttaca tactgagaat acttctatag
     3721 gtacctcaaa tttgtttttt attaatgatt cataggtata tattttccag tctttaacct
     3781 tgacagctcc aacttctaaa atttctgaat ttaaacaatc taatccacta acttctatat
     3841 ctaaaaaaac aacatcattc aatacactta ttatattatc catttttata tcccttcaag
     3901 ttaaaccagc ctaacttatt tacaatcatt tctctttata ttttatcatt tatatacact
     3961 ttatttaaga taattaatat caactttata tcttacagta aacttctttc atttattatt
     4021 tgtatatgtg aatattttta actataaata ttcaaactat attataagaa atatacttta
     4081 aattttaaat aagaggtgaa attgtgaaaa aagatagcct aaaaaaatac attatgatag
     4141 gagcatttgc tctaatactt tttggaattg ctagtataaa ttttaaatca ttagataaaa
     4201 caaaaactca aatttctagt ccaacactcg acacacatga atatgattgg tattttaacc
     4261 ctagggagga tggtaaacag ccatcaccta taaaagaagc tgattttttt aaaaaatacg
     4321 gagcttatta tgtaggaaat cccaatgaaa aagttatata tctatcattt gatgctggat
     4381 atgaaagtgg aaacactcca aaattattag atacgcttaa aaaacacaat gctaaagctc
     4441 aatttttcgt agttgaaagt tatataaaaa gcaatccaga gttaataaag cgtatggaaa
     4501 aagaaggaca tttagtttgt aatcattcaa aaagccatcc atcaatggca ggtattacag
     4561 attttgaaaa attcaaagaa gaaattacaa gtgtagaaaa agcatacaaa gatgttactg
     4621 gaaaagaaat gcctaagtat tttagacctc caatgggtaa atttagcgaa caatctttaa
     4681 aatatacaca agatttagga tataaatcaa tattttggag ttttgcttat gtagattggt
     4741 atgaaaagaa acaacctact catgaatttg ccaaaaacaa aatttattca agaactcatc
     4801 ctggtgcaat agttttactg catccaaatt catcaacaaa tactgagata ttagatgagg
     4861 ttcttacaca ttgggaaaaa gaaggttata aacttaaaac acttgattat ttaaataata
     4921 aaaaatagta tcataaattt taaaaaactc tatcatgaat catagtaaaa atatagttat
     4981 ttataaatac tttaattctc gatagagttt tttatttttc attgcctaaa ataggatttt
     5041 taaatacttg taatcactaa gctttcttgt taaaaactgt ataatatgct aagctatgaa
     5101 caaggagttg acagctatga ctaccatttg tgtaatgatt gaccatatat ttttagatac
     5161 agagtataga caaccaacta tccataaaca tcttgcaaaa catattatta tatcacagtc
     5221 tggtaactta tctgtgacgt ttgataaggg cgaatcatta caatgtaaag gaattattat
     5281 tgattccaat gtatcgcata cggtgtcaag taaagataat aaaatgttgg ttttcttgat
     5341 agatgataca agcatactcg cccaaaatat tagtcaacac caattactaa atcactcttt
     5401 tttcgttata gataatctta tagttgaacg aatacaaaat atttatacag cttgtcaggt
     5461 aaaaggattg tcccaaagct atattgattt ttcaaaacaa gctttccgtc tattgaattt
     5521 aaattatcat atttcgtcta ttacagacag tagaatacaa tgggcattga atcatattct
     5581 aaagtccaca cacattcaca atgaaatctt ttttgagata tgtgccgaag taaaattgtc
     5641 caaaagccgg ttttctcatc ttttcaaaga acaaatggga gtatcactaa acagctattt
     5701 ggctttatca aaacttcgca aggcttatga gttattgtta aaaagtggag atattacctc
     5761 agctgctatg gacgcagggt ttagttcacc gtctcatttc tcagcttcca gcaaaaaata
     5821 tcttggcatt actgcaagtg agcttatagg aaaatgtaaa atatatttta tccgccaata
     5881 gcgtctattt gaaagtcagt acttctaata tttgttacaa taaatgcata gcattaaaga
     5941 ttactcttta ttgctatgca ttatcttttt aggagtgatg acattgaaaa caaataaaat
     6001 ttcttttaat aaaggtctat attaccttaa tacaaatgcc aattttaatt ttcagttaaa
     6061 caggattatt atgtgggatg gggggcgctt ggaagatata caaaaagttt cctctaaaat
     6121 tgtcgatagc gaaagttgga aaacagaact tatgtatctg gcacaagaag ctgaacaaga
     6181 tggacgcaca gaaaatgcaa ttgcttacta tcgtatgagt gaatttttta tgtatgatgg
     6241 tgaccctgat aaaatatcag tctacaaaaa agcaactact ctattctaca actactactc
     6301 tgaatatttc tcaaatggaa ctgtacaaat ttttcatgta ccatatgaaa atatcaccct
     6361 acctgtaatg tatgcaaaag cgcaaggcga ggtaaaagat gttatattgc tccacggtgg
     6421 aaatgattct tactttgaag aattcttttt cccaatgctt tatcttgcgt cacagggatt
     6481 tgatatttat ctttttgagg gacctggtca aggtggagtg atgcgtttgc agggaaaaca
     6541 ctttacttat caatgggaac gtcccgttaa gacaatttta gattattttc atttagagaa
     6601 tataactatt gtaggtgctt ccttgggagg ttttcttgct ccacgagcag cagccttcga
     6661 aaaacgaatt gaacgcgtgg tagcttggtc tgtattcccc aatttcttag acataatagt
     6721 tggttcacaa ccaataaaaa tgcaaaatct acttagacta ctgctaaaac tcaaaacaaa
     6781 atggctagtc aattttataa taaagcaaaa aatgaaaagt ggcgatagta tggtggtatg
     6841 gggtttaaaa catggaatgt acgcatatga agcgtctaca ccctatgaat attttcagaa
     6901 aatcaagtat tatcaaatag attctattgc aaaaaatata aaccaagaca ttttgatttt
     6961 aggtgcaaca caagatcatt ttattgatta tagaactgtt ggtaaagaga ttgatttact
     7021 caacaatgtc cgatccctta catttaggct tatgacagaa gcagaacaag ctagtaacca
     7081 ctgtaactgt ggaaatagca aactggtttt agatacaatt tcaaattgga ttatgatgat
     7141 gaaaatacat gatagagtct aaatattgat ttttaaatta tacaaaatcc atctgctagc
     7201 atatccttat tgctttgttc aaataatcac ttgttaaaaa attggttccc acttacgaaa
     7261 tactacgttt atagaaatga actatatcat gtaagcacac aatatatatg ataataagac
     7321 ccaattactc aatgtaactg ggtctttctt atgtcataga ttttatagga ctcatgaaat
     7381 gtaaaagagt atttcaccga tttccaaagg gaactttact tagataagga gaccataaat
     7441 gctaacaaac ccatctacta gacacctttc aagtatttga gcgattatac aatacacgtt
     7501 aagaattaca cgaaaattta ttatttttat agctatttaa aaagaattgt ccattcattt
     7561 atctctttgt atcctatatt atgatatatt ttacctgcta catcattatt ataaaaaaga
     7621 catggaactt ttccttccaa caataattct ttactcaaat tgtaaaccat ataacttgca
     7681 agtccttttc ccctatatct tttatctgta cttacagaga ccaccattgc caaaaaacta
     7741 gtttccatac cagttgacac agtgctaatc atagtatcat catttctaat aaaatatgct
     7801 cttacatttc cctcttttaa atgttctttc ctttcttcta tgtaattttt acttacttta
     7861 tagtctgacc tatttaaaag ttttcctatt tcatcaatat ctttttcctg agcattttct
     7921 attttatact ccttcatgtt ggaaaaatct atttctttta tttcttttaa tacacaaaat
     7981 ttattatcta gtttttcata aaaatctttg tatttactta ttagtctatc tattacacat
     8041 ttttttccac ttatgctttc tacatctaaa tcttttatat gattcttcat ttctcctata
     8101 tcaaaattat ttttaaaact ataaattata agtgttgact tattttttaa tattatagag
     8161 gttatatttc catcttctac ctgagaccat atatcttgat attgagagct aaatccataa
     8221 ttctcaacgt atccaattat gaataaattt aaactttctt catgtgatac atattgccaa
     8281 aattcttttt ctaaagtatt atctattttt acaatcaagc agatatcctc cttctatgtt
     8341 aataatgata taccttataa tttatagtat catttaaaat cattatattc taccaaatat
     8401 tctaaatttt taaatatcta tgcaatatgt tcgtatttat ttttatattc tgttactgct
     8461 tcactccaag cttcttctac tgaaatacct ctcttatttg caattttttg tgctaattct
     8521 attattttca tagcttttct tgatatattc atatatattc ctcctctaaa ttatcataag
     8581 ttattttaaa gttaaatttc tatagtcatg ttatatattt atttacttat atcttactaa
     8641 ttatttatgt actaaatatg aactacaata gaagaaattt agaacttaat aaaacataaa
     8701 atttatatac agttcatata tatttgtcat atcacaagac aagtgatata atttattatc
     8761 attttactgg gggtgatatg ctatgaaaat aagagctgaa tatacatgtc ctcttgaaat
     8821 tgtacatgac ataatcaaag gaaaatggaa aactatcctt atatttgaat taagacaatc
     8881 taataaaaca ttttctgaat tgcaacataa tatttatgga ataaatcaaa aaatgttgat
     8941 tcaacaatta aaagagttac gtgattttgg gattattgat aaaataagtt ctgctggata
     9001 tccattacat gttgaatatt ttcttactaa tcgtggtaaa aaaatgttaa aagcagtgga
     9061 aataatgcaa gaaataggaa tcgaatatat gcttgaacat ggtcaacaag aaattctaga
     9121 taataaagga atttgctaca ataagtaatc ctatacatac caaaaagtaa gtaatttaca
     9181 ttttttacaa tagtattata ataaaagtaa atagaaggta taatcatgtc tatagcttta
     9241 atattgattg atattcaaaa tgattatttt aaaaatggaa aatgtgaatt atttcaatca
     9301 gaagaaactg ctgaaaatgc taaaaaaata ctactgtttt ttagaaaaaa caatttacca
     9361 gttattcata tacagcatat atcaacagat aaaacagctt ctttttttct tcctaatact
     9421 catggttcag aaataaataa aattgttttt ccactagata atgaagaaat tataattaaa
     9481 cacacaccag atagtttctt tgaaacagat ttgcaaagta tccttgagaa aaaaaatatt
     9541 actaatctag taatctgtgg aatgatgagc catatgtgta ttgatacaag tgtaagaaca
     9601 gcaaagaaac ttcgatacga cataactcta ataagcgatg catgtacaac gaagaattta
     9661 tcttggaata atatagaaat taatgcaaat actgtacatc aagtattcat ggcatcacta
     9721 cagaacagtt ttgcagatgt aaaaaacaca gataaatttt tatctaacta tcacaaataa
     9781 aaacatacac tatgttaaaa gagcaagttt gatttattta agcttgctct ttaatcattt
     9841 aaaattagat gctttctatc atttttatat acataatata cgtttataca ttttatattc
     9901 atatataact attatataaa ttttaaactt tactatattc acataaaaaa tgtagtatta
     9961 tagtatatat aatattatat agaagggggt gctagataaa tgaaccatca tatattgatt
    10021 caatttgaat atatagcaag aatcattact gctggtttct gtggggcttt aattggatat
    10081 gagagaaaga atagactaaa agaagctgga atacgcactc attttatagt ggcattagct
    10141 tcagcactta taatcattgt ttccaagtat ggatttaatg atatagtggg tactccaggt
    10201 attggtctag acccagcccg cgttgcagca caagttgtca gtggtatagg ttttttaggt
    10261 gctggattaa tatttattag aaatcaatca gtaagtggtc ttactacagc tgctggtata
    10321 tgggcaacag ccggtacagg tcttgccatt ggctctggat tatacttagt tggtattgtt
    10381 tcaagtatca ttattgttat tgtacagata acacttcata gagataggaa gtggatgaaa
    10441 ctccctacag cagagcaagt ttctcttaaa atttctaaca gcactgattc catagaatat
    10501 attgaaggta aatttaaaaa atctaatatt gaaattataa acatgaatat taaacgcctt
    10561 gatggggatt ggttagaagt agttatatat actaagttac ctaaaggata taaaaaaaca
    10621 aatttattaa aattatttag cgataatcct cttatagaaa ctttagaaat ataatataaa
    10681 aattgaagct taaaaataat aatatattta atcaaggtca atatataaat aattacggga
    10741 tgagtctata taaaattcat cccttgtttt ttgccattca tatatatttt ttcaagatat
    10801 ttttgctcat ctattatcta gccaagtaac tcttgtgaac taaacttttt aatacaaaaa
    10861 cttcaataca aaactaaata taatttttat taaatattct acatgtatag tttgtttctt
    10921 gagtgaaaaa tagatttcat attgttaaga tactgttaaa tatttcaatt ttctgacaag
    10981 tatgttgaac aataacttat cctagtgtat tattttagtt gtagaataaa actaaatcgg
    11041 caaaactaga gaaatttagt gacgcaaagc tatagggact aacgcttata aaataaacta
    11101 tgagttatgt cagccagttg ccaaaaagat attgttcttt ttgttttatg aaagtttttt
    11161 agggggatag taatatatat gtttagagat gaaatggata aatgtacaca catgttaact
    11221 gcttatatta gtagtttata tgattattgt gattttatag atacacagct agatgatttt
    11281 atactagagt acggagaaaa tgtagtagaa tcttgtttac atcaagtgat ggtattggta
    11341 agtaagtata attaaataga aattgttttc tagagaaccc tttttagttt tatctacatt
    11401 taaaaatgtt attatattaa aaaactaatc tcccctatat gcaaagtttt cttccatata
    11461 acaatttctt cccatttata ctttttcact tgtatcttta ataaaattca acttcaaata
    11521 ttaatatact tcaaaaatta ttctatttct tatctttaca ccaattccta tcagtattat
    11581 cctttatata ggttctaact atttcgccac aatctaaaca taaatctgaa tatatagtag
    11641 attgtactgg aaaagtttta tttataactt tatctaattt tccatctcct ctatatttta
    11701 atccaatagt ttcagcatat atgccaccag ttgttaaaat acccttttca atatttacac
    11761 ttccacaatt agaacatttc acatttataa ccccctaaac aattattttt tcatgctatc
    11821 ttataattta aaatataatt tgctaccgat tttataacac agtagcaaat cggtggcaaa
    11881 tcaacatttt ttcattctat ttaccatcta atccattgaa atatggctat ttagttatag
    11941 gaacttgaat ttccgttaca aactcttcta caattccagt ctcatgtata tctatcttat
    12001 agatttctat agggtcagca attatcttat aattattttc ttctataaat ttaaacacct
    12061 tctccatata catcttatta ttagaattac tcccagtata atttaaagtt acataatatc
    12121 cttcatcaaa aactatatta tatactttct cattactttc cagaaaacaa aatacagatt
    12181 taaactcatt aaatatacct ttattaattg cttcagtaga aaaggcagaa ccaatattgt
    12241 tgtttccaag tatattaaat ctatcttcat attctttctg aagtttctga attaaaaaat
    12301 cgaagtcttc atccctcttt atatcagcat ttaattctaa agcttttctt ttttctatat
    12361 atacaacctc tatctcatct atttttgtat tttgaattac actttcaatc gaactcaatc
    12421 tttttataat attttcctta tggcttataa attcagctat ttttttatca attaaatcga
    12481 gttcttcatt taaaatattt ttcgtagact ctatacttct gtcatctagg tattctttta
    12541 ttttcttcat tggaaagttc aagcttctaa attcttttat taaatttaat ttccatatat
    12601 ctgatatatt atacattcta tatccattta tatctctaac tggtcttaga atgcctattt
    12661 cttcataata cattaatgaa tctctaccta ttccatatat tttagatatc tctcctattt
    12721 tatagtaatc tttcatttat ataataaact cctccgaaat cctattttta ttttaaatca
    12781 acattattac tctataatac tataatatta gaatatacta aaaaaatctt taaaacaagg
    12841 ataggaaaat aaacatccta a
//
LOCUS       DAMKPG010000100        12026 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMKPG010000100 DAMKPG010000000
VERSION     DAMKPG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.105"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            238..1212
                     /locus_tag="PC621_003015"
     CDS             238..1212
                     /locus_tag="PC621_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110156.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1328..2359)
                     /locus_tag="PC621_003016"
     CDS             complement(1328..2359)
                     /locus_tag="PC621_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF4110157.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(2971..3822)
                     /locus_tag="PC621_003017"
     CDS             complement(2971..3822)
                     /locus_tag="PC621_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4110158.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3839..4759)
                     /locus_tag="PC621_003018"
     CDS             complement(3839..4759)
                     /locus_tag="PC621_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF4110159.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4869..5783)
                     /gene="murQ"
                     /locus_tag="PC621_003019"
     CDS             complement(4869..5783)
                     /gene="murQ"
                     /locus_tag="PC621_003019"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF4110160.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5882..6979)
                     /locus_tag="PC621_003020"
     CDS             complement(5882..6979)
                     /locus_tag="PC621_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF4110161.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(7212..8558)
                     /locus_tag="PC621_003021"
     CDS             complement(7212..8558)
                     /locus_tag="PC621_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4110162.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8647..8955)
                     /locus_tag="PC621_003022"
     CDS             complement(8647..8955)
                     /locus_tag="PC621_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4110163.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9216..9605)
                     /locus_tag="PC621_003023"
     CDS             complement(9216..9605)
                     /locus_tag="PC621_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110164.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(9607..10767)
                     /locus_tag="PC621_003024"
     CDS             complement(9607..10767)
                     /locus_tag="PC621_003024"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF4110165.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11197..>12026)
                     /locus_tag="PC621_003025"
     CDS             complement(11197..>12026)
                     /locus_tag="PC621_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4110166.1"
                     /translation="SYINLYNCIENSGEIDSYLIDGQSFEENLIKDIIENLKIKNNSD
                     IETYKQSLKELDDYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKINFLVNS
                     ILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDVYTDSKC
                     MSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRVFDKGFT
                     GKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDYLSLDS"
BASE COUNT         3846 a         2107 c         1326 g         4747 t
ORIGIN      
        1 ttttcatttg ctatttaaaa tcaattttaa gttttcttct tataataaac ttcctctttg
       61 tggtctcatg ttagtctcta aatttaaaat tttacaaaaa attttcataa aataaaatta
      121 tattgattgt atctaaacaa atagggcaat aaaataatta agtatagtta cttacctttc
      181 aagcaatatt agttttatgt tagaatttta ttatattgat ttacaggagg tttgcatttg
      241 aataatatta attcacttaa agtaataaaa ttatctcttg gatgttctct tgctatattc
      301 attgcttggc tattaaaatt agaatattca atggttgctg gagttatagt actacttaca
      361 gtaaaggaca ctaaaagaga aactcttaaa ggctccattg gaaagattta tggttttctt
      421 ttatgtacta ttttttcata tttatgtttt aatattttag gatataattt gacttcattt
      481 tcaatattta tatttataat tataccactt tgttttttgt taaatattca agatgttatt
      541 gctatgtgtg tagtcatagc atcccattac taccttcaag gagaaacttc tatcaaatgg
      601 attttaaatg aagctggaat atttgcagta ggaactggaa taggtgtctt aataaatatg
      661 tatattccaa caaatataca taaaatccat gaagttcaaa aaaagttaca ggaagaagtg
      721 agtattgtac ttatagacat tgcagatatt attgtaaatc ctaaaaaaga aaatggatat
      781 agtagagatt tatataaatt aaattcactt attgacagca gtatttctga aacttacgat
      841 aatattaata atactttatt aagtgatact agattcttct tagaacatat ggatattata
      901 aaaagccaac gggatatctt agaaaatttg tatagttatg tatctcaact aaattctaca
      961 ccaccacaag ctcacattct atctgctttc atacataaaa taggttatac tgaatttgaa
     1021 gctgaaacag gcaatcttct tcttgaagaa ttgaagagat taatgcttag tatgaaaaac
     1081 catcctctac ctgttgacag aacagagttt gaaaacagag ctattttatt cctatgcttg
     1141 actgagttaa aacaattttt agttaatcgt aaacatgctc agatgcttag agataataat
     1201 ttttataaat aaaaaggtga gaaatccagc agttgacctg actacaaaat atcagatata
     1261 atattaaatg cgcccctagt aagagggcgc gatgtttata acttattcta ctgtcatgat
     1321 taaatcatta ttcatatcga atatacatta tttgaccaag tccaatatta gctttttcat
     1381 cagtgatttc tattttaata gtcacatcat tttttttaca gtactttgct aattctgcac
     1441 cagaacgatt tttaccacta aaatctaaag aaaatggctt atcatattta aaaggatatt
     1501 caacaggttc accttctggt gctaatgcaa acatcccata tggtgtgttc ataaatccta
     1561 ttacagcaat tgtaagattt gatgggtcta aatttgattt actacctaga aatgtaactg
     1621 tattcactga tgaattaatt acaacacatc taacaccttc atacattaca tgtttatcct
     1681 ttatatctcc aaattttcca acaacctttg ccccgtcttg atgtggtaac atagtgtgct
     1741 tcccaggtag tgctggtttt tgcgtttgtg ctactgctcc aactgttgga tatgctgtct
     1801 tatcaaatgg taatgggtgg ctaagactct tgtatatgat atcctttcct gctgatttat
     1861 tagcatcaat atctacatga tacaaacgcc catcaccaaa acgtataaga ttccactggt
     1921 ggtcatttga tgtgctgtgt acataataag attcaattcc catagcgtca cacaaagctt
     1981 tcatgctcct agccattcct gaacatgcac cttttttagt aaccaatgta taggcagtat
     2041 tcgctccttc tactgcattc cagtcaacat atgttgtgtt atttttaatg tgattatagg
     2101 caactaaaat cttagcgtct gtagtcatgt tctttgtaat tttattgttc ataaaatctg
     2161 ctgttgcttg tgctactgag tttagttggg cttgtgacag ctttggacca tatacgctat
     2221 tggttccagt gacatagctt tttggtgttt ttgctgttac ctctttggct gtcataccat
     2281 aaactgtcat actcatacct gagactaaaa caattgttgt aatcaataac ttggtagcta
     2341 acttcctaat tttcttcata cttagtattc tcctttataa aatattgtta cttcctattc
     2401 tctaccatta ataaagttca tttgggattt aaaaaggtgg attgtcaata tgtatgtttc
     2461 ttcatttata cgttcgtgaa atatttaaat ttcatggaaa atctaataga tattttctag
     2521 ataatttgta tacaatacaa ctcgcaaatc agggtgtttg ctgttagttt gagaaaatga
     2581 tgatgattaa tatattcgga atctttgacc tataataaat accattgtct tcaatcatta
     2641 ataatggtaa gagtaaagag aattatcaat aatacagtta aaatcaactt tgtatacatt
     2701 tcatacattc tctcccaaca acattatact tcttataatt tgtatattca atacgttttc
     2761 tgggaaaata taggatatat ctataaaaat ataaaaaagg agttgaactt aaaatttgat
     2821 taaaaaaaat caattttaag acaactcatt tgtattataa aaatgctata tattattatt
     2881 aagtaagtca atattttaga tatttatcta tttcttattg acttacgctt atagtctaac
     2941 ttatttgttt agttgatata taacctagtt ttaagcatta ttaatattta aataatcaag
     3001 tatccttttt gtttcttgta gtttctgttc tgcctcattt aagttttctt gaattataga
     3061 aatatatatt atatctatca acgtaagcat tgctattctt gaagaaatag ctccttctct
     3121 aagccttttt tctatatttg gtactactag ctttaaatca ctaatcttac taagatgatt
     3181 gtcactatat ttagttatac aaatgacctt acatcctttt aattttgcat tttctataga
     3241 ctttattact tcttttgatt ctccagagta agatattgct atagctatat cttctttatc
     3301 tacatttgaa gataccataa gttgtgtatg actatctaaa gaggtaaatg cctgtttatt
     3361 tatccttaaa agtttcatct gtaaatctag tgcaactaaa gccgaaccac ctactccaaa
     3421 tatatataca tttttagcct ttattatctg ttcaactgct tcttttatta tatctttttc
     3481 attcaacaac cttgtttggt tgatagtctc tatgtttttg tttgtaattt tgtttataac
     3541 actatctata ttacaatcat agttttcatc tacatatgtg tattcatttt ctttgcccat
     3601 caaatcttta gctatctcaa tttttaaatc cccaaaaccc gaatatccca tttttctaga
     3661 aaacctaact atacttgctg tacttgtttt tgagtttgac gccaattctg atacagaaag
     3721 cttacttata gcctctgtat tattaagtat gtaatcagct atttttttat ctacttccgt
     3781 taaatcttca aatatctgtt ttaattttaa aatgcaactc atactctacc acctctacct
     3841 atttactata atatttcttc ttagcaagta aacatgctcc taaatctgga gtcaacactg
     3901 gtctcactat accaaatctc gttttattca aatatttttt aagtggctct aatattaaat
     3961 ctcctgactt aaacactcca cctgaataag atacctttat ctttgtacct tgttcaaaat
     4021 gatgctctaa tcctattata agcctactta attcataagc tgcatcatca aatattttac
     4081 tagcagtatt atcaccttca ataactgctt ttaaacatat cgtagcaaac tttgctattt
     4141 ctattctatc tccttgtatt ccatcattta catattttat tatgtcataa tcattctcta
     4201 tattaagatg tctttttatt aaatcatata attttgtctt tgaacacctt ccatcagact
     4261 gcattgtgaa catttttaag gtagccattc ctatataata cgctgaacct tcatctccta
     4321 atacatagcc ccatcctcca catctgtgaa gattcccatc tttatctaaa gcctgtgcta
     4381 ttgaaccagt acctgcaatt atatttatac ctttttcccc atttaaagaa cctgctaatg
     4441 ctatttctac atcattacct agagtataat ttatatgact atatgcattt tttgttgcta
     4501 cttctaatgc atacaatact tcttttattt ttccatatcc agcaagtcca acaaatgcat
     4561 acgttatttc ttctacattt atcttagcat ctttacaaac ttcttccaac ccagttatta
     4621 ataatttcgt tagattatca aatcctattt gattataatg acaagttcct ttggttatgg
     4681 ttcctactat atttaattct tcatctacta atgtaaatgt tgttttcgtt cctccaccat
     4741 ctacacctaa aaaatacata ctatcctcct acaatatctt taataattct ttcaatactt
     4801 aatatctaaa aataaccttc caatgctgtt tttttaatat tgaaaggtta tcagtactat
     4861 tatttatttt acaatttttc tattgatttt aaagcttcac ttatataacc ttcttgtcta
     4921 tctaggatac tctttgcact atccttatct aaaccacttt ttatcataaa tatagccaat
     4981 ttaactctat tatctgcttc ttctaagaat ttttctgcaa cgtcatattt tacatttgta
     5041 gctatcatta ttattctttt tgctctttca accaactttt catttgatgt cttaacatct
     5101 accatcaagt ttgagtatac ttttcccatc ttaaccatag aagctgttgt taacatatta
     5161 cataccattt tttgagctgt accacttttc attctagttg aacccattat tacttctgcc
     5221 cctactataa tgtttattgg taaatccgct tttttactca tttcactatt cggattcata
     5281 gttattgata gtactgcaca ccccaattcc ttagcatact ccattgcacc tataacataa
     5341 ggagtccttc ctgatgcagc tataccacat ataacatcct tagatgtaac tcctctatct
     5401 attatatctt gctttccaag ttctttatca tcttcagcac cttctattgc tttaaatatt
     5461 gcttgatttc caccagctat tatagcttga acaagctcaa aatccactcc gtaagttgga
     5521 ggacattctg tggcatctag tattcctatc ctcccactgg tacctgctcc tatatagaat
     5581 aatctacctc cctgcttcat tctatctact attatattaa cagcttttgc tatactctcc
     5641 ttttgttctt caacagcata ggctacagtt ttatcttcgt tatttattaa ttctattatg
     5701 cctaatgtat cttctttatc tatttgcaat gtattcttat ttctaccttc tgtaactaaa
     5761 ttttctaaag tttttattaa catatcattt cctcattttc ttaatattaa atttttaaac
     5821 acattttaac tccaactttc ataattatat ttttataata aattaatatt ttcataataa
     5881 attattttgt tgagggttta aaatcaaact tttgccatgg tttaatataa tctaatataa
     5941 ataattcttc ttttcttata tatcctacta cattcgattt accactgttt ttcatatcag
     6001 tcaaggctat ttgtaactcc cctgcataat gaccatattc actactttca attactatat
     6061 ctcctctttt tattatttta ggagtattaa atagttcaaa tttgtatcct ttatatttaa
     6121 cacgagattg agtagacctt atcagactgt cagatatatc ccctctatta aaatgtattt
     6181 cttcaaagag tatcttcttc tcaacttctc gtatattttc ctctattgct acatcaaatg
     6241 taaccatatc ttttctcata gaacctaata cctttagctc ctcatatgtt ggataacaat
     6301 ttgatattat tatatcattt atattattca tagctattat atgctttact tgaatatcta
     6361 taggtagatt tctgtgcatc tcaattgttg gaagcccatc agttacaggc catggtccaa
     6421 atgtggtttc attttgactt gttacaaaaa ctgctgttct taatccatac ttattaaatc
     6481 tttctgtaca ttttataaag tgttctaaat ttaatccact atatctatgt ggataaaagt
     6541 tatgacatcc tgtcaaattt tctttatttg gttggtagtc cattattgta tctatataat
     6601 tagtattatt actcatattg atttctattt taagattttg aggattaaaa gtcattaagg
     6661 ccttttcaga acctgtaaat cccatatcaa gccttattcc gtctgccttc gtctttttaa
     6721 aaaaagtcaa atctttgtag cttatttcta attcaccaaa tactctggga tttacatcaa
     6781 gtattacctc aaaaccttta aacttagcgt attcatttat acttttaaat tttttaatta
     6841 tattttcttt agtatcttta actgataata aacatgaaaa tatacgtgta aatccatttt
     6901 cagcagcttt atctatataa tcatatattt ccttagatgt tgttttttct ggataaattg
     6961 atattcctaa tcttcccatc tgtgtcatct ccttatatcc tatatgtgtt taaatttatt
     7021 tcgaattaca cgaaactttt ttaaaaattt ggtgaaaaaa agtttcatat atatttataa
     7081 aaaaagaaaa gtttaccttt tcttttttta taaacaatca acaaaatatc ttcgttactt
     7141 atttcataac tgttatactt tgaaatttat actaatctaa tatagttttt aatttccaat
     7201 atagtttcta attaataagc ttcttgctct tcaacttttt cttcatgaag caatctctta
     7261 tcgtacatct tgacaaatgg atagtaaatt attattgata tagctataca taataagtta
     7321 agaactgaag ctctccaatc tccaccagtg gctaaatatg cccctactgg tcctggtaat
     7381 gtccaaggag cagtaactac tactctggat actaaattag cactcatagc aaaccatgaa
     7441 actgttgcca ttactattgg tgccacaaca aatggtatta ttaatattgg gtttaatact
     7501 attggtgctc caaatattat tggttcattt atattgaata ttgctggtat tacagttgct
     7561 tttcctaaag actttccata agctgatttt gctctaaatg caagaagtaa agctaagcct
     7621 attgtagctc cagaacctcc aatccatata aaccattgaa aaaagggttc agcagcaata
     7681 cctggaactg cttgacccgc agcatatgaa gctgtattag catctaataa ttgtatccat
     7741 aaaggtcttg ccaatgaacc tattattgat acaccatgta ttccaaatga ccaaaaaaac
     7801 gttattaaaa atattagtat aattactgat ggtaatgtgt cagtggctga aactaatgga
     7861 gttactaatt tagctatgaa gccatgccaa tcaaacccta ggaaataagt aacaatagcc
     7921 attaaaatta ttattaatgc ggctggtgtc agagcttcaa aagaccttgt gactgatgct
     7981 ggaactgctt ctggcatggt tatcttgaat ttagatttgt cagtaaatct ataaatctca
     8041 acacctatta tagatgttac aatagcaaca aacattgaac ctccaccaag attagccatt
     8101 ggcatttgcc atccttgagg aattgaatta taccaagcta aaagttctgg actcttttca
     8161 actatcttta ataaatcagg ggtaagaccc tctattgcag taggaactat tgtcaaaagg
     8221 aatgtagctg tagctaataa acctcctgtc aatccatcta acttatatga ctttgccaaa
     8281 ctataaccta tgccaaatgt agaatatact gatataataa aaaatgtcat tctatatggt
     8341 agtaatattg taactgcatt ttcctttaga tactgagtta ccccccatga atctggtaat
     8401 ggaggaaatg ctaatatcaa gaaaaatgac ccaactatta taagtggtaa tgaagctacg
     8461 attccatctc ttacagctct taagtgacgt tgttctgcca atctggccat agaagttgat
     8521 aatttactgt ctaaaaattc tgtaaattta tctagcatct tttctctcct taattaatat
     8581 ttatttttat tatcattaaa atttttataa aattaacttt cttctaacct aacaacactg
     8641 caactactat ttactcaaca actcttgaac ttgttttaat aactttggtc ctcctaatgg
     8701 actataagct tgtgcttgta ttaatgcaca tggtacacta gcctcatcag caaaagcttt
     8761 taaatagtca aatctatgtc tgacttgtgg agctaccata gcaatatccc atccattttt
     8821 aacttcattc tcaaactctt gagtaccaac agccaaaaca tctatatcta ttccattttt
     8881 tgctccttct ttttgtaatg cattaactgc tatagcactt gacatccctg atgaacaaac
     8941 aaataatact ctcatattga ttccccctgt ttttttattt cttttcaatc attatagcat
     9001 gtatttttga ttacaggaat acgtttgtat atattttttc atattttaca tttttaattg
     9061 taattttatt tcatgcattt atatcttgct ttaattgctt tatttatcta atcattcttt
     9121 tgtctaaaat tacacactac ttaaaatact catatctttt cttaaaaaag aaaaaagact
     9181 atcttttatt aacataaaat agtctcaata cttttttaaa tatgtattcc tttatatatc
     9241 cagaagtaac ctattattgc aaatgataaa acaaataaaa tatacagtct aaaaaatccc
     9301 aaagaaaaac ctctcttagt ttcaacaaag tatcttgtat atgcaactgc aaaagatgta
     9361 attaatgaat ttcctataac aactccaaat ctattatcta ttccaaatat agataataac
     9421 aaaggaaaaa ctacagtact tactagagat gaaactatta gtattaatcc acttttaaaa
     9481 cttatagttg gaatattata atctgttctt tgatttttct ccatttcttt cttagtattt
     9541 ttattacttt ttgcattttt ataattatta tttttctttc ttttcttttt tttattatta
     9601 gacatgtcat ttacctccaa aaggtttagg tgttatattc cctaaaataa ctttattttt
     9661 agcccctgtt gcagatatta cattagaaaa agaattattt aatgtttcat tgcccaagat
     9721 tacaaatgcc aaagcctcct ttgcatcaga agaataccca tattcatctt gagttaatac
     9781 ttcaacctct aataataact cttttatata tcctattaaa gttagattat gaactcctcc
     9841 acctccaatt aataaagtat ctacattaac ctttggaagt atgaagtttt tataattata
     9901 atatatagct tttgccgtaa acatagttac tgttgctatc aaatcatatt tatctacatg
     9961 tttatatttg ttaagcatgt tatccaaata gacttgtcca aatacctctc ttccagtgct
    10021 tttaggtggc gcttgactaa gatacttatg actcatcaaa tcttttaaca tttcttcatt
    10081 aattttacct tttgatgcaa agtatccatt tttatcatac tttctattaa ataatctttg
    10141 acatactcca tctattatca tatttcctgg tcctgtatca aatgcaaata catcatctat
    10201 gttacatgat ttaggtatta tagtaatatt tcctattcca ccaatatttt gaagtgctaa
    10261 atttttattt ttatcactat ataataaaaa ttcactatat ggtactaatg gagcaccttc
    10321 tccacctgct gctatatcca ttactctaaa attagatatt acctttgtat ttgtttcata
    10381 tgctataatt gctggttcac ctatttgaag cgttgaagat acaaaattat tataacttct
    10441 tggtatatga aagattgttt gaccatgaga tgcgataaaa tccaaatttg ctatatgaaa
    10501 gtttgcttta tgacacactg attttacagc tttactaaat aaatatccaa gcttaaaatt
    10561 taagctacag attaaatcaa cactcgattc ctcttctata cagcactttc ttatttcgtc
    10621 ttttatatct ttaggaattt ctagagttat aaattctatt tgcttaactt tagtatttct
    10681 accattccca ctaatttcag ctagtactgc atcaactcca tctaaggaag ttcctgacat
    10741 taaacctaca ctatacatag ttatcatcct tttaagttta tagttattcc tccccaaatc
    10801 aaggtattaa gtttaataat tttagaatta atcttatttc atctttaatt gcgataattt
    10861 actcttacga aattaatcga gttaatctta tacacgtttt ttaccttagc taaatcttac
    10921 attaatttat tttcagtgtc aatgtaatgc atgtaatttt ttttcacaaa caaaacatat
    10981 tctctctcca aaaaccatat taaaatagtt actacaaata aaaaacatac caggtaccta
    11041 tctgatatgt ttttcatata taataattat tattttataa tatagataga acatcattta
    11101 aggcactaat taaatcttca ctattttcaa gaactaaatt tattcttatt gtatatgtcg
    11161 atttgtatat gatgatagat tttatagtct aataacctaa ctatctaggc ttaaataatc
    11221 cgatatatca taaaagtaga tattaaaaat tgtatatgaa ccaattttcg aacatacctc
    11281 aatctcatgc cctaaactat ctatcatttt ctttacaaaa tacatcccca ttccagtaga
    11341 cttatacaat tgatttcttc catttttccc cgtaaatccc ttgtcaaaaa ccctagatat
    11401 atcttcctca tttataccta taccaaaatc ttttatagat aataccactc cattttctaa
    11461 cttttttgaa ttaaattcaa tcttaccaac ttcctttgaa tactttatag cattattaat
    11521 aatttggtct aaaacatatg acatgcattt agaatctgtg taaacatcat tttctaattc
    11581 attcaaacta acttctacat tattttttat aagcaaaaat gaattatttt ttatagacat
    11641 ttttaccaat ttttctaaat taaatttatt tataaataca tcttcaaaca tagtgttgct
    11701 tctactacta tacaaaatag agtttactaa aaaatttatc tttgcaacct gattcttaat
    11761 atccaaacta tcttctatgc tacttaacct atctgtaatt atacttaaag aagatattgg
    11821 aatttttatt tcatgaaccc atttagctat gtaatcatct aattctttta atgattgttt
    11881 ataagtctct atgtcactat tattttttat ttttaagttt tctataatat cttttattag
    11941 attttcttca aaagattgtc catcaattaa ataactgtct atttcaccac tattttctat
    12001 acaattatat aaatttatat aactat
//
LOCUS       DAMKPG010000101        11907 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMKPG010000101 DAMKPG010000000
VERSION     DAMKPG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11907
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.106"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            266..730
                     /locus_tag="PC621_003026"
     CDS             266..730
                     /locus_tag="PC621_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF4110167.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            737..1231
                     /locus_tag="PC621_003027"
     CDS             737..1231
                     /locus_tag="PC621_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF4110168.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1247..2272
                     /locus_tag="PC621_003028"
     CDS             1247..2272
                     /locus_tag="PC621_003028"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF4110169.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2259..4706
                     /locus_tag="PC621_003029"
     CDS             2259..4706
                     /locus_tag="PC621_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF4110170.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4886..6259
                     /gene="radA"
                     /locus_tag="PC621_003030"
     CDS             4886..6259
                     /gene="radA"
                     /locus_tag="PC621_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF4110171.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6262..7332
                     /gene="disA"
                     /locus_tag="PC621_003031"
     CDS             6262..7332
                     /gene="disA"
                     /locus_tag="PC621_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF4110172.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7421..8515
                     /locus_tag="PC621_003032"
     CDS             7421..8515
                     /locus_tag="PC621_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF4110173.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8644..10821
                     /locus_tag="PC621_003033"
     CDS             8644..10821
                     /locus_tag="PC621_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110174.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(11007..11852)
                     /locus_tag="PC621_003034"
     CDS             complement(11007..11852)
                     /locus_tag="PC621_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110175.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
BASE COUNT         4927 a         1217 c         2140 g         3623 t
ORIGIN      
        1 caaatgttta gtaataaaga tgagaaggta tatttaaaat ataaagtatt taaagttctg
       61 aaatctaatc atgaagcaaa aaattagcat ttggttatca ataaatataa taaataaaaa
      121 ctcataatta atgaaagaat aaattcatat ctaaatttct ataaaaaatt taaatttata
      181 caatttaggg ctttaaaaat ttaaataata ggtgtaaaat aatagtcaaa gaaagtcaaa
      241 aataaaaagg tgtgtggtga tattaatggc aactatgaca gatataattg aaaaatttat
      301 taaagatttg atggaagaag ataatagcat acaaattcaa agaaatgaac tagcaaactt
      361 gtttagttgt gcaccatcac aaataaatta tgtcttaaca acaagattta caatagatag
      421 aggatattac attgaaagta aaaaaggtgg cggaggatat gttcaaatag aaaaaatacg
      481 aaaaagtaaa gatggacata ttagagaact actaaatgaa aaaataggaa gtcagatatc
      541 ctataaaaaa gcaaaggaat tattagaagg tttaaaagag tcggatttga ttaacgaaag
      601 agaattaaaa ttaatattat atgcaataga tgataaatca ttgtgtatgc caatatatga
      661 attaaaagag aaggtaaggt ctaatatttt aaagaatata ataataggat tatttagtat
      721 agaggagtga taatttatgc tatgtcaaaa atgcaataag aataaagcat ctgtatatta
      781 taataagata gtcaatggag aaaaaacaga gatgtattta tgtagtgagt gtgcaaaaga
      841 gaatacagaa atgaatttta acttagatat gcctttttca atgatggata tattttctaa
      901 tttaggtttt caacctaaaa aagaacttga ggaaaagcta gtttgtccta aatgcaatac
      961 aacatatagt gagtttaaga ataatgggag atttggatgt agtgaatgtt ataatgcatt
     1021 tagtagtcaa gtaaatccaa tgcttcaaaa tatacatggg catatagaac atacaggaaa
     1081 agcacctaaa aaatcttttt ataagataag tgtagaaaat gagattaaag aactaaaaga
     1141 agatttagat agagctataa aaaatgaaga gtatgaatta gcagctcaat ttagagataa
     1201 aattaaatat ttaaaaggtt ctattgacta agattggagt gatattatga aagagaacat
     1261 agtcatgaaa tcaagagtta ggctagctag aaatttaaat aactatccat ttccaaataa
     1321 attagataaa gaatgtgcca tggaaataat agaaaaagta aaaaatgcgt ttattaattc
     1381 taacttagaa caaaaagaag aatttgattt ctataaaatt gaagatttag accaatctaa
     1441 aaaaatgctt atggttgaag aacatataat aagcccagat ttagcagaaa atgataaaag
     1501 tgcagttata gtaaaaaaag ataaaactat aagcataatg ataaatgaag aagaccatat
     1561 aaggatacaa actatatgtg atgatttaaa cttagaatat gcatatagtg tagcaaatga
     1621 aatagatgat ttgttagaat caagtttaga atatgccttt aacacaaagc taggttatct
     1681 aacatcttgt ccaactaata caggaacagg aatgagagct tctgtaatga tgcatttacc
     1741 agcattaagt caactaggct atatggatga actttataaa atttcatcac aaataggtat
     1801 agcaattaga gggatatatg gagaacgaac agaagcattg ggaaatatat atcaaatatc
     1861 taatcaactt acacttggaa gaacagagtc taatattata gaaaatgtaa gtggacttac
     1921 aaaagatgca atttcaaaag aaataaaagc tagagaaata ttgcaaaaaa aattaggtat
     1981 aaaactagaa gataaaatat ttagatcgat agggacactt gaaaattcaa gagtaatgag
     2041 tagcgcagag gcaatgagcc atctatcaaa tataaaaatg ggaatagaaa tgaactatat
     2101 agataaacta gatttaaggg ctatagaaca acttatgata ggtatacaac cagcgcatca
     2161 gtcaataatg tataagagtg atgatgttga gaatagagat ataaatagag caacatatat
     2221 tagagaaact ttggaaaaat taagaggagg gatgaattgt gaacttcaat agatttacac
     2281 aaagagctaa aaaggcaatt gatttagcgt ttgaatctgc taaaagtcta ggacacaata
     2341 ttgttggaag tgaacatata cttttaggtc ttttaagaga agaagaaggt atagctgcta
     2401 aagttctaag taaagttgga tttacagaag cttatttaga aggcaaaata gttgatatgg
     2461 aaggtaaggg agaagaaatc tcagaagata tagtattaag tccaaggagt aaacaaatac
     2521 ttgaattatc aggaatgttt gcaaataaat taaaaacaaa ttatattggg actgagcata
     2581 tattattagc aattattcaa gaaggtgaag gaatagctaa taagatttta aattatgctg
     2641 gagtaaatga taggacttta gcccaattaa caattgatat gatgggtatt agtgataaaa
     2701 atcaatacaa agcagagaat agttacacgg gcaaccagaa tcaagcagaa tctaaagtac
     2761 ttgataagta tggaagaaat cttacactat atgcaaaaca gaataaaata gaccctgtaa
     2821 taggtagaga aaaagaaata caaagagtaa tacaaatatt aagtagaaga actaaaaaca
     2881 atccagtgtt gataggagac ccaggtgtag ggaaaacagc catagctgaa ggtctggcta
     2941 caaatatagc cttaggaaat gttccagaaa cactaaaaaa caaaacttta tactctttag
     3001 agatgggttc attattggct ggggctaagt atagaggaga atttgaagaa agaattaaag
     3061 aagttgtaga tgaagttgtt aaaaatggaa acataatctt atttatagat gagatgcaca
     3121 caataatagg ggctgggtct actggagaag gctctataga tgcttcaaat atattaaagc
     3181 cagccttggc aagaggagaa atacaagtta taggtgcaac tacaattgat gaatatagaa
     3241 agcatgttga aaaagattct gctctagaaa gaagattcca accagttatg gtagatgaac
     3301 caactaaaga ggactcaata aaaatattag aaggattaag agataaatat gaagctcatc
     3361 ataaagttaa aataactgat gatgctataa aggcagctgt agaattatca actagatata
     3421 tatcagacag atatctgcca gataaagcta tagacttgat tgatgaagct gcatcaaaag
     3481 taagattaaa agaaaataca cctccttcag aaataaaaaa attagaacta gaaatagaaa
     3541 atatagataa agaaaaagaa gaagcagtaa gatgtcaaga ctttgagaaa gcagccaaaa
     3601 taagagatga acaaggtcta cttaaaaaac aattagaaga tgttagagaa agatggaaca
     3661 agtcatctaa acattcagat ttagttgatg gagaagttat tgctgaagta gtgggcttat
     3721 ggacaggaat accagttaat aaaattcttg aagaagaggc tgatagactt ttgagacttg
     3781 aggaaatact gcacaataga gttataggtc aagaacaagc agtaaaatct atttcaaagg
     3841 caatcagaag gtcaagagca ggtcttaaag accctaatag accaattggt tcatttttat
     3901 ttttaggtcc aacaggagta ggtaaaacag aattatctaa ggcattagca gaagtgcaat
     3961 ttggagatga aaatcaaata attagaatag atatgtctga atatatggaa aaacatgctg
     4021 tgtcaagaat gataggttca cctccaggtt atgtaggtca tgatgaaggt ggtcaattaa
     4081 ctgaaaaagt aagaagaaat ccatattcag ttatcttatt tgatgagata gaaaaagctc
     4141 atccagatgt atttaatatt ctgttacaaa ttctagatga tggtagacta actgattcaa
     4201 aaggaagaac tgtagatttt aagaatacta ttgtaataat gacatcaaat gttggagcat
     4261 ctacaattgg tagacaaaaa actttaggat ttagtatagc taaaggagac gaagaagaaa
     4321 aatctcaata tgaaaaaatg aaagaaaata taatgggtga attaaaacaa agattcagac
     4381 cagagttttt aaacagaata gatgatataa ttgttttcca ctcactaaat gaaaatcata
     4441 tatcaaaaat agtactttta atggcagcga aactacaaga gagattaaaa gaaatggata
     4501 taaaattaga aatgagtgat gaggctgtta aattaatttc taaatctgga tttgacttag
     4561 aatatggagc aagacctctt aaaagagctt tacaaaaaga gttggaagat gagctatctg
     4621 aagcaatctt aaaaggtaat gtgaaaaagg gaagtaatgt agtagcaaaa gtcaaagatg
     4681 aaaaaatagt ttttgaaact aaataattta atagagttgc attttaaaat attgcaaata
     4741 taagttttat aatctaaaca aaattttaag ggcaatgaat ctataataat tcattgtcct
     4801 taattatttt atttaatctt tacatattgt ctttcagata tgctaaaatt atagtataga
     4861 ataaaaaatt aaggatgata aaagtatggc aaaaataaaa acaaaatatg tatgtcaatc
     4921 ttgtggatat gaaacagcta aatggcttgg taaatgtcca gaatgtacaa aatggaatac
     4981 atttgtagaa gaaatagacc aaaaaagcac caaaaaagag gtctttataa tagataaatc
     5041 ttcatctaaa ccagtcagta taaactcaat agaaagtaaa gaagaagaga ggtttacaac
     5101 agatataaat gaattagata gagtgcttgg aggaggtata gttaaaggct ctttagtgct
     5161 tgttggtggt gaccctggta taggtaaatc aacactttta atccaagtat ctagtaatgt
     5221 tgccaattta ggaaaaactg ttttatatat aactggagaa gaatcagaat ctcaaataaa
     5281 aatgagagct aaaagattag gtattaactc agaaaattta tatatatttg ctgaaaataa
     5341 tttaagtata attgaatctt atttagaaag tgtaaatcca gaattaataa ttctagactc
     5401 aattcaaact gtcttcagtc cagaaatatc ctcggcacca ggaactgtca gccaaattaa
     5461 agaaggtact tcaaaattca tgaaaatttc taaaaaaatg ggaatttcaa cgttcatagt
     5521 tgggcatgtt acaaaagaag gttcactagc aggaccaaaa ttattagagc atatggttga
     5581 tactgttcta tattttgaag gagaaagata taatacttat agattagtaa gagctgttaa
     5641 aaatcgattt ggttcaacta atgaacttgg agtatttgaa atgagggatt taggattagt
     5701 agaacttgat aatccatcta aaattttaat ctctgaaaaa ccaaaagatg tagcaggttc
     5761 agtaataata tctactgtag aaggtacgag accaatgtta cttgaattgc aagcattagt
     5821 ttctcctaca agttttggta taccaaaaag aacatctaca ggagtcgatt acaatagagt
     5881 aggtatgtta ttagcagtat tagaaaaacg tgttggtttg caaatacaaa atcaagatgt
     5941 ctatataaac atagttggag gaataaagat aaatgaaccc tcaattgatt tagggatagc
     6001 catttctgta gcatctagtt ttagaaatat accgatagat gaaaatatag ctgttacagg
     6061 agaagtggga cttacaggag aagttagagc agtaagcttt atagaaaaga gaatagctga
     6121 gtgtaaaaaa cttggattta aaaagatagt agttcctaga agtaattatg atgttgtaaa
     6181 agagaccaaa ggcatagaaa tatggccagt agataactta agacaagcta taaatatagt
     6241 acttgggagg aatcaataaa tatggagaat tttctagata ataaaaatat gctatatgca
     6301 ttaaaaatga tatctcctgg aactccactt agattaggtc taaacaatgt actaagagct
     6361 aagactggtg gattaattgt aattgcaaca aacgaagatg taatgaaaat agtagatgga
     6421 ggatttgcta taaatgcaga atattcacca tcatatctat atgaattagc taaaatggat
     6481 ggagctatag ttttaagtgg tgatgtaaag aaaatattat ttgctaatgc acaacttata
     6541 cctgactatt ttatagaaac atcagagaca ggaacaagac atagaacagc agaaagagta
     6601 gcaaaacaaa ctggtgctat agtcatagga atttcacaaa gaagaaatgt tataacagtt
     6661 tatagaggaa atgagaagta tgtagtcgaa gatatatcta agatatttac taaggcaaat
     6721 caggctatac aaactctgga aaaatataag acagtattgg accaagctgt aacaaattta
     6781 aatgccttag agtttaatga tttggtaact atttatgatg ttgcattagt catgcaaaag
     6841 atggaaatgg taatgagagt tacaagtata attgaaaaat atgtgataga attgggtgat
     6901 gaaggaactt tagtaagtat gcaattagaa gaattaatgg gtacaaccag aatagaccag
     6961 aaattaatat tcaaagatta taataaagaa aacacagaaa taaaagaact tatgaaaaag
     7021 gtcaaaaatt taaattcaga agaactaata gaattggtca atatggcaaa actattaggg
     7081 tatagtggtt tttcagaaag tatggatatg cctataaaaa caagaggtta taggattctt
     7141 agcaaaatac atagactacc aacagcaata atagaaaact tagtaaatta ttttgaaaac
     7201 tttcaacaaa ttttagatgc atctattgaa gaattagatg aggttgaagg aataggtgaa
     7261 ataagagcaa catatataaa aaatggactc ataaaaatga aacaattagt cttattagat
     7321 agacacatat gatgaatttt aaatgaattt tactactttt ttcttgaata taatatacac
     7381 tttggtctat aatagataag tataactagg gggtgaagca ttgataagga aagtaacaag
     7441 aatattgttt attttacttg gatttacaat aggtataaca acctatttaa cattaatgaa
     7501 agattttgaa atattaacat ttggaaaaga aacatatggt tatatcgcag ctgtagtagc
     7561 aggtataata atagccattt taggatatct tatagaacca tgggttgtaa ataaagtaaa
     7621 agaaattgct aaaatagtgg ataaagaatt gtcaaaatat cctcagactg atatattgtt
     7681 agggtctatg ggtcttatag taggatttgt tattgcgtat ttattaagtg gattagtaaa
     7741 tagaatacca atagtaggtg gaatactatc cttactatta tatttattcc taggatatct
     7801 tggaatgaaa gttgcgctta aaagtaaaaa tgatttattt aatattggaa aactaggaag
     7861 attagcaaat cctattaagg ataaagataa ggataaagaa aataaaaaag aagtaaaagc
     7921 cattcctcca aaagtattag atacaagtgt aataattgat ggaagaatag ctgatatttg
     7981 taagactggg tttatagaag gaaaacttat tattcctgct tttgttttgg aggaactaag
     8041 acatattgca gattcttcag atgatttaaa aagagtaaga ggtagaagag gtctggatat
     8101 attaaatata attcagaaag aattaaatat agaagttgaa ataagtgaga gagactttga
     8161 tgatatagca gaagttgata gtaaattatt aaagcttgct caggttttaa atggaaaagt
     8221 tgttacaaat gattataact taaataaagt tgctcaattc caaggtgttg aagtattaaa
     8281 tataaatgag ttagctaatg ctataaaacc agttgctata ccaggtgaag atatggttgt
     8341 tcaagtggtt aaagaaggaa aagaggctca acaaggtgtt gcatatctag atgatggaac
     8401 tatgatagtt gttgatggag gaagaaaata tatgaatgag actataaaag tactcgttac
     8461 ttctgttctt caaactcctg caggtagaat gatatttgca aaacctaaaa attaatatgt
     8521 tcaaagaaac ctataatttg ggctgtttat tttaaataga tggtccaaat ttatttattt
     8581 ataacgataa tgtttaatgt attcttaaag atggggattt aaatcagaaa gcagggagaa
     8641 tttatgagaa aaaagattat atctttctta gcaacattta tcataatatt aacatcagca
     8701 agtcagtata gttttgcaga tgatatgtca acacgtggaa aagtaatatt tatagatatg
     8761 aatagaacta gtatgagtaa tatgttaaga ataaaatcat taagagaaga actagacaat
     8821 agagggtata ttggcttgat gaatataaga ggcgataaag gaagtgatga tagaaggtca
     8881 tatgctagta tgggtgctgg tggtagagct aatgtggcta atgaagaaga tattaatttt
     8941 gaaagctcga gtaaggatag aaatatagtt tttgaatcag ctacaggaaa aagtgcaaaa
     9001 ggtataaata atttaactat aaataagtct ataaatgaaa atttaaactt tggagagtat
     9061 ggttctgtat tagggtcatt aggacaaagt ttatctgaga atggattaaa agcatctgta
     9121 ttgggtaatt cagatataat tgaaaatggt caacttataa aaaatagaaa cttatgtcta
     9181 actgcaatgg atgaatatgg aagaatacct aatggaaatg tagatactat aaataaaaaa
     9241 gacttatcta tgccttatgg aatatcaact gattatgata aattgattgt agagaccaaa
     9301 gaagcttaca aaaataatga tgtggttttt gtggagcttg gagacacata tagattagat
     9361 ttatataaac caaatttaaa tgaaaaaaca tatgagagtg tgaaagataa catagaaaaa
     9421 aatatagatg tatatttaaa aaatatattt tctatggttg aagaaaatga cactgtctac
     9481 atagcaagtg catttccaag tgatttagat tataaaaata aaagaagact ttctccaata
     9541 ataaaattaa atggagaagg gaaaggtata ttatcatctt caactactag aagagaaggt
     9601 attgtaacaa atttagatgt tggggttgaa atattagaca actttaatat aaaaaatcaa
     9661 aatatggttg gaagaaaata tgaacttatt aacagagatg ataataaaga atttttgatg
     9721 gatgaatatc aaaagatagt atctatttca agcataaggt ctacaatact gaatggtttt
     9781 gtaagtatag ttttcttgtc atggatagtt gctatgattg caattttatt tagaaagcat
     9841 atatcaaaaa attataaaga aacaacattt tttatattaa aggaactttt aaaaatagga
     9901 atagtaatgc cactgtcatt tatgataaca ccaataatga atttcaaaac accattagca
     9961 atatctttag gaataattat aataacactt actatatatc ttataagtaa agttttaata
    10021 aagaatgatt taaaaaacat gctgtttttt actggattaa ccatagtaat tatggttatt
    10081 gatgctgggt ttggctcata tttaatgaaa agcaatgtta tgagttatga ctgtataatt
    10141 ggagctagat attatggagt gggaaatgag tatcaaggtg tggcaatcgg tagtgcaata
    10201 tttacatttg caattttact tacatataaa aatataccaa agtggtctat aatagttttt
    10261 tctttggtta tcttaattac aagtgcatct ccaattatgg gagcaaatgt tggaagtgct
    10321 atttctgaat gtgttgcatt tttactattt atactattga tatataatgt gaagatagac
    10381 ttcaaaaaaa ttgtattatt aggtatagca gtattatttg tattaggagt ttttgtagca
    10441 atagatatga ttttgggaag taattcacat ttaggtatgt ttgtaaaaga aatttacttt
    10501 aatggaccag gagaaataat acaaacattt agtagaaaga tagagatgaa tttaaagcta
    10561 gctcaaacca gtgcatgggt aaatatactg ttaactggaa taggtgtaat tttagttctt
    10621 atgattaatc agattagata ctttaagcaa ctgatggatg agtatccaat tgtatttaaa
    10681 ggatttatag catcaatagc aggatgttta gtaactcttt tagtgaatga ctctgggatt
    10741 gtgtcatcag ctacagcctt tatttatgta attgtaccta tgataacatt aagtataaat
    10801 ctcacagcat taaaagaata agtactgtat gaataatatg taaatatgaa cacattaatt
    10861 taacttcttt atttacaaaa taaagaagaa agtttataaa aaataaatta taataaaaaa
    10921 gagtttacct caaaattgat tattctaatc aaattttaag gtaaactttt tttattataa
    10981 agataaataa catataacct catattttag gtagattttt ttcttatatg tttaggagtg
    11041 catttaaatt tttccttaaa aattctgtaa aataattttg tattagtaaa tccatgagct
    11101 tctaatatac tgcataaggt atcatttgtg ttaattaagt ctttataaat atgagaaata
    11161 cgcacatcat ttaaatactc taagtaagtt tgtcccatat actgtttaaa ttttctacaa
    11221 aaatattctg gctgtaagtg tgctacctta gcgattttgt taatagaaat aggcattttg
    11281 taattaatct ttgtatattc taaaactggt tcaaattttg acaaatcaag tactgttttt
    11341 ttcatatttt tattacctac agatatttta aagtttgtat ataattcaaa taaaagttca
    11401 aataataagc ttgtaaagag cagattagct gcaggaggag agctaagttt aatttctctc
    11461 atgttttcta atataagttt aactttacct gaattttttt tatagttatt gtcattggca
    11521 ttaaccttaa agtcaaaata gcaattttta aaatcaggga tatatttgtt tagaaaagac
    11581 aatggtactt gcaataatat agaggtatct tgataagtac atctagttga gtgaataatt
    11641 ccagaattaa tgaacataca ctcattagct tttaaattat aagtgtaacc ctccatgtta
    11701 atttgtaaat caccagaagt tatataaatt atttctaatg agttatgcca atgatttgct
    11761 acataactac cactatcatc aaatagtttg aaattaacat ctaaattatc aataggttca
    11821 attatttcat gtgaaaaatt atttctgttc ataataatat cctccttata attttaaatt
    11881 ataaatagta aatttataat gttcaat
//
LOCUS       DAMKPG010000102        11862 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMKPG010000102 DAMKPG010000000
VERSION     DAMKPG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.107"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(611..1438)
                     /locus_tag="PC621_003035"
     CDS             complement(611..1438)
                     /locus_tag="PC621_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF4110176.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            1820..2140
                     /gene="sugE"
                     /locus_tag="PC621_003036"
     CDS             1820..2140
                     /gene="sugE"
                     /locus_tag="PC621_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF4110177.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            complement(2273..2953)
                     /locus_tag="PC621_003037"
     CDS             complement(2273..2953)
                     /locus_tag="PC621_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF4110178.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            3349..4401
                     /locus_tag="PC621_003038"
     CDS             3349..4401
                     /locus_tag="PC621_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF4110179.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4448..4864
                     /locus_tag="PC621_003039"
     CDS             4448..4864
                     /locus_tag="PC621_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4110180.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            4893..5366
                     /locus_tag="PC621_003040"
     CDS             4893..5366
                     /locus_tag="PC621_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4110181.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            5413..6171
                     /locus_tag="PC621_003041"
     CDS             5413..6171
                     /locus_tag="PC621_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4110182.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            6195..7034
                     /locus_tag="PC621_003042"
     CDS             6195..7034
                     /locus_tag="PC621_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF4110183.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            7485..8132
                     /locus_tag="PC621_003043"
     CDS             7485..8132
                     /locus_tag="PC621_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110184.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            8125..9519
                     /locus_tag="PC621_003044"
     CDS             8125..9519
                     /locus_tag="PC621_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110185.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            9944..>11862
                     /locus_tag="PC621_003045"
     CDS             9944..>11862
                     /locus_tag="PC621_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF4110186.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHAKATEGTAE
                     GNYAVGSKATYKTAI"
BASE COUNT         4654 a         1288 c         2109 g         3811 t
ORIGIN      
        1 ctgatgaaat cttataattt aagttgaaaa tggataaata gaaaggaagg attttaatta
       61 aaaaggtaga ttaattaaaa agtatataaa ttataaaaat gaattaatct acattaatta
      121 gttgatatat caattattat tattttttca gttgcttaaa atatacttga tatatcaact
      181 aatgttgttt tttaattaaa agaaatttta ggaaagataa tattaactag gagagataat
      241 atcaacattg aagaaggtag agcaatattt ttacataatt gtgaacatta tctggcatgt
      301 gtgcattggt gttttaagca agcaattcaa tagaaagatg tgactaaaaa tagaaagaaa
      361 taccataatc cacatgtaaa gctagaagac ataataagta aaaaaagtaa agatatttaa
      421 taaaagaaat aactaatagt gattaaatta gtaaatgctg acaattagca taatcattag
      481 taattattag tattatttaa ttaaattaat tttagcatta tttttattaa attaatttta
      541 aatcaaatta atgagaaccc cataaatttt tttaacactt aatttatgat ttatggggtt
      601 atgttctgtt ttatttcaaa gtattaagtt catataaatc agttcttctg tgcttaagaa
      661 gtggtaactg ttgtcttatg tcaggaatta aatctctatc aatgtcagca taaataatac
      721 attctttttc atcagcttga gcaattattc ttccccaagg gtctgaaatt cttgaatttc
      781 caaaagctat ataagaagca ttcatatttc tagcaggtgc agcaccaaca tagaatatct
      841 gattatctaa agcacgcatt cttatagata actcccaatg ggcgggacca gtagtcatat
      901 taaatgcagc aggtaagata acgatttcaa cacctttaag agccattaaa cgtgataact
      961 cagggaaacg aatatcatag caaatagcta taccaacttt tccatattcg gtatctatta
     1021 cagtgacatc atttccagct gtaagagtat ctgattcttt aaaactaact tttcctgtaa
     1081 cttcaatgtc aaataagtgc attttacgat gttttccaat tagagctcca tttttatcaa
     1141 atacatagca agtattatag atttttccat attctaactc aggaatacta ccagaaacta
     1201 gataaattcc atgtttctca gctaatttac tcaatatcgt tgttgtttca cctggatatt
     1261 cttcagcaaa tttaggaaaa tatttatttt catatgggca gttaaacatt tctggcaaaa
     1321 ctataatttt agctccattg atggcagctt ctgttaccat atctacagca tgattgatat
     1381 tttcaatttt attttctgtg gtcatcatct gacatactgc aattttatag aaattcatag
     1441 aaattactcc tttcaatatt tttatgttat tataacatat taaataaaaa ttagatattc
     1501 ttagattgac ctaaatgggt atggagagaa gtgaataaga atattaaaag gtatataaaa
     1561 gttattttta ttatagatgg aacccatata ttgacttttt ttatatatgt tgatagtatt
     1621 attatagata aaatagaata attaatagag ttttataggg ttttgcaatt attaatgatt
     1681 ggcctggtcc gagataaaac ccacaacata gtttttgttg tgaacacgga aggacaaaaa
     1741 cctgggagat attttataat atttctcagg ttattttatt ggaaaaataa gagatacatt
     1801 tgaaaagagg gatgagttta tgaaatggtt atttttgttt gttgcaggtt tgtttgaagt
     1861 gttttgggct tttcaactaa aaaattcaca tggattttct aaattaactc caagtatact
     1921 tactgttgtt ggaatgattg taagttttta ttttttgtca ttagcactaa aacatcttcc
     1981 acttggaact tcatatgcaa tatggactgg aattggtacc gtcggtacag ctattgtagg
     2041 catggctatc ttaaatgagc ctattagtat atcaagaatt gcatgtatag gctttattgt
     2101 ggtgggaatt gtagggttaa agctattatc tactaattag agtatattaa tttaaataag
     2161 ctatgaaatt aaaggcgaga aaatgtttaa aaataatcaa catttaaaaa gtctttactt
     2221 catagcttat cttatttata agtttattat atttgaaaat tatatgtata tactatttat
     2281 ctaataaaag agcagttaaa tccatagggt caactattac accatcttta taaacacgca
     2341 tatttccaga tgctatttca tctattaaga ttacttcatc attgtagaat ccaaattcaa
     2401 atttgatatc ataaagttca agaccttttt cagctatagt atcacgaact atattagaaa
     2461 ttttttgagt catgcttttc attgaatcaa attgagcttg agtcataatg tttaaagctt
     2521 ctaaaccttc agatgttata agagggtcac aacggtcatc atcttttaaa gttgcttcta
     2581 catagcaatc tagtttagca ccttcttcaa cacatgaacc ataacgacgt aagaaactac
     2641 caacagctct ataacgacaa ataacttcta atccattacc aaaaactttt gctggtagaa
     2701 cttccatagt tgcttcttct aaattagcac ttacataatg agttttaatt ccagcattgt
     2761 ttagaatttc aaagaactta acagatgtct ctaaattagc tctaccaatt ccatctatac
     2821 ttagacctac actattttcg cctgggtcaa atactccatc tttaccagtt acatcgtctt
     2881 taaatttaag taagtaattt ccattatcta attcaaaaac atctttagtc tttccttgat
     2941 acactttttt cataaacaac ctccaaaaag ttaaattatt atattgaata aggcattagt
     3001 gtcaaaaaag ttctgatatt cgcatgaaaa tttatattga taccttatat ttaatattat
     3061 atcacaaata gaagggaaat ttccgaacaa ttttataaaa ataacaaaaa aagtacattt
     3121 tataaaactt ataataagag aactaatata aaatgttagt gtcgtttata ttgacacaca
     3181 taagttttaa tataagaaac ttttgacaca ctttgacaca catttaatga taattttata
     3241 aaatgcatga atgcaatgct tacatatatt ggcatgaaag ttgctttaga tatttaacat
     3301 ataaacaata gggatatatt ctcaaataaa aagtaggggt ggttaatagt gaagtcagtt
     3361 agattctatg gtataagaga cacaagagtg gaggatgtgg atgtaccaaa aatattagaa
     3421 aaagatgatg taataattaa agtaaaagta gctggaatat gtggttctga tatatcaaaa
     3481 tattcaaaga caggtccaca tatggttgga gaaattttag ggcatgaatt ttctggagaa
     3541 gtggcacagg ttggtaaaga agtaaggagt tttaaaatag gagatagagt tgctgtttgt
     3601 ccagctatgc catgttttga atgtgatgaa tgtaaaaaag gattgtattc aagatgtaat
     3661 aatgtagcaa taataggaaa taaagaactt ggaggttgtt ttgcagagta tacaaaggtg
     3721 aaagagagga atttaataaa aataccagat gagataagtt atgagactgc cgctgcgcta
     3781 gaaccagtat gtatagcagg gcatggtctt tttagatcag aagctaaagt tggagatact
     3841 gttgtagtac ttggtacagg tacaatagga ttattttcaa tacaatgggc aaagatattt
     3901 ggctctacaa aaataatagc tgtagatgta tttgatgaga agttagactt ggcgaaagag
     3961 ttaggggcag atatttgtat aaatgcaaaa gaaaagaata ttgtagaaga aataaaaagg
     4021 ttaactgatg gagatggtgc agatattgtt attgaatcgg ctgggactcc aatgacatgt
     4081 gggcaagtat tgttgttagc taaaaaggga gggacagtat tgtatgcagg tgtaccatat
     4141 ggggatgtag ctttgactag agaacaattt gaaaagatag taagaagtga attaactgtc
     4201 aagggaactt ggtttggaaa ctcttttcca ttcccaggaa aagaatggag tgctggattg
     4261 tatcatatgc aaaagggtga tatgaatgtt gaaaaattag taacacatag aataaattta
     4321 gaagaagtac cagcttattt tgaaaaagta tacaaaaggg atattttctt tggtaagata
     4381 atgattaata tagataatta gattagagta ctaaataata atccatataa acaaggttaa
     4441 gaaaggtatg attcatatga cagggataat tatagccaca catggaaata tggctaaagc
     4501 aatgttagaa agtgctgaat taatagtagg caaacaggaa aatgtagaaa ctttaggatt
     4561 aaaccatggt gacagcatag atgagtttag tagaaggtta gaagatagaa ttgagaaata
     4621 taaggatgaa ggggttttag tattgcttga tttttatggc gggactccat ttaatacatc
     4681 tgctattata ataaataaat ttgctaaggt atgtgagtta gaatgtttga caggtgtaaa
     4741 tttacctatg cttttagagc tatttttaaa cagagatact atgaagttaa aagacttgaa
     4801 gaatttatgt gaggaagttg ggatatcagg aatcaaagat gttaaaactg ttttaaagtt
     4861 gtaaaataaa ttaaaaatct ggaggaataa ttatgagtaa tatagcatta acaagaattg
     4921 atgatagatt aatacatggg caagttatta ctgcttggtg taagattaca tcggcaaaaa
     4981 ggatagtaat cgtggatgat ttagtagtaa aggacccatt tatagttcaa gttttacaaa
     5041 tggcagctcc atcaactgtt aaggttgaag tacatgatgt agaatcaggt gctgaagttt
     5101 taaaatcaca taatggagat gaacatttaa tagtattggt caaataccct aaaactgtct
     5161 taggtttagt aaatagtggg gtagatttaa aggaattgaa tgttggaggt atgggagcag
     5221 ggcttggtag aaaaagtttt tataaaaata tatctgtgtc agatgaagaa aaagaaatat
     5281 ttagagaact aatatcaaaa gatgttaaat gctttataca aatagtacca gatgcaaaga
     5341 aaattgatgt aggaacatta ttataaaaaa gactatcttg taaatcttta aaagaaaata
     5401 gggaggaaga atatgcacat ttcactaatt caagcagtat taatagcaat tttttattat
     5461 ctttcttgga gtccgtggtt gacctatgtt ggatttttta catggaatag acctctattg
     5521 gctggatttg taacgggaat tatattagga gaccctgttc aaggagcaat tataggagca
     5581 ggtataaata tgatatatct aggttttata tcagcagggg gagctcagat gggggaccca
     5641 gcttttgcag gatatgtagg tactgcttta gctatagcat ctaagctaga tgtaagtact
     5701 gcaatggcaa tagctgtacc attgggaact gttgcaactg tactttggat tggaaaaatg
     5761 acagtaaatt ctttttttgc acattgggct gatagagagg ttcaaaaagg gaatgttgat
     5821 agagtagcat ttataaatat agtaccacca cagatactgt tatttgcaat gagttttata
     5881 ccagcattat tagttgttta ttttggtcca aatgcgatag atggtatgtt ggaagtaatg
     5941 aatgagaatg tgttacatgt atttaatgta attggggcta tgttgccagc acttgggatt
     6001 gctatgaatt taaaattgat tggaaacaag tttactatgc cctttttcat attaggaata
     6061 ctaatggctg tttattttaa agtagatata atagtaatat ctgttattgg agtaatactt
     6121 gctcttacaa ttacttcaat taagtacgga aaagatagtg ctacatctta aatacatgtg
     6181 agggggaaaa tattatgtca gatataaaaa atgaagtatc taagaaaacg ttaagcaaaa
     6241 aagatgtaat aaaatcatgg ttaagatggt ttttctttgc gcaatctaat tacaattatg
     6301 aaagattgca atcaacagct ttctcacatt caatgttgcc agtacttaaa aaattatatc
     6361 cagataagga agagttaaaa caagaagtgg agactcattt agcatttttt aacacagaac
     6421 ctatatgtgg atgtgttata catggaataa caatagctat ggaagaagaa aaagctaatg
     6481 gagcagatga aataagtgga gatggtatga atgctatcaa aacaggcttg atgggtccac
     6541 tagcaggaat tggtgatact ttaactcaag gagttataac tcctatagtg ttagctgttt
     6601 gtattggttt aactgaggga ggagcatcaa ttgcaggtcc tatattattt gtaatagctc
     6661 aatatataat aatgacttca atttcttttg gaatgtggac aaatggatat aagtatggga
     6721 aaaaagctgt tgaaagtata cttcaaggtg gaatagtaaa taaggttata gaaggggctt
     6781 ctatattagg tactttagtc atggggggac ttgtaggtag gtttattaat ttatctacac
     6841 caataagtta tactagtggt gattttaaat ttagtcttca aacggattta cttgataaga
     6901 tatttccagg attaattcct ttggtactta ctttattagt actctttgct ttaaaaaaag
     6961 gcctttctcc aataaaaata atgtttatac tgatagtagt gggagctatt acaggtattt
     7021 tgggactatt ttaaataaat atatatgttg gtgtgtaaat taggattcta tatctattga
     7081 ttatatttgt aagatgttgg aaaatttata ttaaaaagtt tagcaattag ggtaatttta
     7141 taatagatta aattttaaac ttatataagc tatatatagt atttaatatt ttaaaaagta
     7201 tctcagaaag atttttaatc agaatgaggt gctttttcat ttgcatataa aatattaagt
     7261 aatttcaaaa aaatataaat ttatttaatt taattaaaat attataatat attagttaca
     7321 tattaaaata tataatcgag atattgttaa gacattgtcg agatattata atatataact
     7381 aatgtattat aaatagttgt aaacttcctg taacatatat tttatataat taaagtaatg
     7441 gatataatat gaaatataat aatgaatttt agggatgtga ataaatgaac atattaattg
     7501 ctgatgatga aattagtatg cttaagatac tttgtgcata ttttaaaaaa gaaaacttca
     7561 atgtgtttac tgcaaaaaat ggggaagagg ctttagatat tttttatgaa aataagatag
     7621 acttagctat attagactgg atgatgccaa taattgatgg aatagaggtt tgtaaagaga
     7681 taaaggaaaa tagtaataca agggtactta tgttgactgc caaaagtcaa agtgaagatg
     7741 aaatagaagc actcaacatt ggagcagatg aatatataaa aaaaccattt gatcctagag
     7801 ttttaataat aagagctaag aaactaataa attataaaaa tacaattaat attaaagatt
     7861 taaaaataga ttttgggtca agtaaagttt ttaaaggtga taaggaatta ttattgacta
     7921 agaaagaatt agaattaatt cgttgtttaa tcaataataa aggaatggtt ttatctaggg
     7981 aaaaactttt agatttagta tggggattgg attatgaggg agattttaga acagtagata
     8041 ctcatataag aagattaagg gtaaaaatag gagaagatat aataaaaact tatagaggat
     8101 tagggtatag tctggaggat tttaatgact aaactaaaca aaaaattgtc cataagtata
     8161 tctatagtag ttgtagttgt ttatataata tctataatta taaatagtat gtttattcat
     8221 agatattatt tacatgaaaa aagaaatata ttggataatg ttgaaaatga aattaaagat
     8281 aaagacatag ataaattaaa atcagacatt actttaattg aagaaaaaaa taatatagat
     8341 atcgtatata tagaattaga taaaaatcat aaagataaag gtaatataga taaaataaat
     8401 caagatttat taaatgcatt ttggaaaaaa ggtttaagtt taaataagtt ttggattgaa
     8461 gaaaatagtt taaataatat tgataagaaa agtataaata agatatacaa tcaaggtaaa
     8521 acaaaatata gtttgcttgt aaaatttatc aagaaggata attatttgtt tgctatatct
     8581 atacctatag aacattctga agaaactatt ggcattgtaa atagatttaa tataataatg
     8641 ggaatttttt cagtagttat aataactata cttacattta tattatcaaa cagagttata
     8701 aaacctatgg aaaggttaaa gtttttatct aaagatatat ctgaattaaa ttttaggact
     8761 gaagatataa aaacgaacga tgaaatagaa gagctagctt acagcataaa cataatgagc
     8821 gaaaaacttg aaaaagcaca taatgaattg aataaaagaa atgagaatct aaaaactttt
     8881 atatcagatg catctcatga aatgaaaact ccaatagcac ttataaaagc atatgcaata
     8941 ggtatgaaag atggtttgga tgatgggact tatacagata caataattga acaagcagaa
     9001 aatatgacca atacaataaa ttctctttta tattgggcaa aatatgagaa gaaggagaga
     9061 agtctgtgtc tggtagattt aaaggaaagg ttatataaaa gtttaaaaaa ctatgagctg
     9121 ttgatagaaa gaggtagtat aagtgttaaa tgcagtgtag atgataaaga acttataata
     9181 agttcggatg aagatagtat agatatggta tttaataatt tgattagtaa tgctataaag
     9241 tatactaata ataatgaaat agagattaat ctttttgaag agaatgagag aattatatta
     9301 tccataaaaa atggtataga ttacaatatg ggagatgata tagaaaatat gtggaagcct
     9361 ttttatgttt tagagaagtc gagaagtaag gagctttctg gtactggtct tggtcttacc
     9421 atagttagaa ctatacttga agaaaataat tttagataca gggttgaaat ttgcgatgga
     9481 attatagaat tttatattat ctttataaaa gtaatttgaa ttaaaatgta aatataaaga
     9541 ataattaaaa aaatatcgca attatttgtt tttcatatgg ataaaattgg ttatttaata
     9601 tataattaca agtataaata ataaaattta tttgtaaaaa gagtagcact tttaattcta
     9661 aaggctactt tttttataca aaaccatgtt ttttataaca attcattaac ataaaaatca
     9721 aacttacctt aaaatataaa tttacaaatt tttcaatcaa taagaaaatt tctctattcc
     9781 ttacataatg actgtaatgt aacaaaataa ttacaaatta attatgaaat cattatagtt
     9841 agaaacctct ttaaaattag atattaagta tcctttagaa ttagaacggg aacaaagtta
     9901 aaaaattaag taatatcaaa tggacaaggg ggaagaaaat aaaatgaaaa taccaaagaa
     9961 gattgctgca atgcttacag ttacaatgat agctggaagt tcaacagcag gcatagctag
    10021 tgcacaaact gtggcaacaa atttaacagg gcaagaaaga tatgaaactg ctgttaaaat
    10081 aagtcaagat ggatggaaaa atgctgatga ggtagtaatc gtaaacgact catctatagc
    10141 agatgctcta tctgcaacac catttgcaaa agcaaaaaat gctccaatac ttttaacaag
    10201 taaagataaa ttaaatgata agacgaaagc agaaatacaa agattaaaag ctaaaaaagt
    10261 atacttaata ggtggaactt ctgtattaag tacaaatata gaaaaagaaa taaaagattt
    10321 aaaaatatct tttgagagaa tctctggagc tgagagatat caaacatcct tagaattagc
    10381 aaagaaatta gatgctataa gtgatgttaa gaaaatagct gtagttaatg gtgaaaaagg
    10441 acttgcagat gctgtaagtg taggagcacc agctgcacaa aacaatatgc cagtaatact
    10501 tgctgattct aaaaatggaa cagcagttgc agacaaattt ataaaagatg ctggaataac
    10561 tcaatcttat gtagtaggtg gagagagttc aatttctgaa gctgttaaaa ataaactacc
    10621 aaatagtaca agactaggtg gaacagatag aaatgataca aatgcaaaag taataaaaga
    10681 attttataaa aagacagatt taaaaaatgc ttatgtaact aaagatggta tgaataaaca
    10741 agaccaactt atagatgcac tagcagtagg tgtattaggt gctaaaaatc aatcaccagt
    10801 agttttagtt ggaaagaact tatcagcttc acaaaaatct ctagttaact ctaagagttt
    10861 tgataagata actaaagttg gtgggaatgg taatgagaca gcatttaatg aaatgaagtc
    10921 attacaagaa gtaaaaacag ttgaagctaa aacaatttct gaattaaaat cagctataga
    10981 taaagctact gctaatgatg ttataaactt taagccaact agtgaggtta aagaagcatt
    11041 tacaatacaa actgataagg cgataacagt aaacttaaat ggtacatata caaagactgt
    11101 gactatcaac atgcctaatg gtgatgtaaa taactatgca aaagtggatg atgtagttat
    11161 agatgatgta aaagatggaa cttttgttaa ctacggaaaa ataactaact taaaggttaa
    11221 tgataaaaat ggagccaaga tagaaaataa ttctaaaggt gagattggta gtttaacagt
    11281 agcatctgga gcaagtcaag taaaagtgac taatggtggt aagataacta cagttacaaa
    11341 taattctaaa ggaactacta tagataataa aggtacaata agtagtgtta agggagataa
    11401 ttctccaaca ataagtggaa atagtccaag ttctaactct tcaggaggaa gttcatcaag
    11461 tggaggttct tcacatggag gaggaagttc ttcatcaaaa gtagataaag tagttttgaa
    11521 aaatacaata acagcagcaa ataaattgta taatgaagca atagagggaa caaatgtagg
    11581 tgaatataaa gtagggtcaa aagcaatata taaaacagca atagacaaag cacaggcaat
    11641 attagacaaa tctggtgtaa cacaaaaaga agtaaatgat gcagtaacag cattaaacac
    11701 agcaacagac acatttaaag caggaaaagt agtagcagta gataaaacag cattacaaga
    11761 tgcagtaaca gcagcaacag cattacatgc taaagcaaca gaaggaacag cagaaggaaa
    11821 ctatgctgta ggatcaaaag caacgtataa aacagcaata ga
//
LOCUS       DAMKPG010000103        11712 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMKPG010000103 DAMKPG010000000
VERSION     DAMKPG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.108"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            205..1530
                     /gene="cdeA"
                     /locus_tag="PC621_003046"
     CDS             205..1530
                     /gene="cdeA"
                     /locus_tag="PC621_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF4110187.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(1625..2419)
                     /locus_tag="PC621_003047"
     CDS             complement(1625..2419)
                     /locus_tag="PC621_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110188.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2420..3295)
                     /locus_tag="PC621_003048"
     CDS             complement(2420..3295)
                     /locus_tag="PC621_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110189.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3299..3667)
                     /locus_tag="PC621_003049"
     CDS             complement(3299..3667)
                     /locus_tag="PC621_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4110190.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3850..4509)
                     /gene="deoC"
                     /locus_tag="PC621_003050"
     CDS             complement(3850..4509)
                     /gene="deoC"
                     /locus_tag="PC621_003050"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF4110191.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4545..6089)
                     /gene="murJ"
                     /locus_tag="PC621_003051"
     CDS             complement(4545..6089)
                     /gene="murJ"
                     /locus_tag="PC621_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF4110192.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(6563..7447)
                     /locus_tag="PC621_003052"
     CDS             complement(6563..7447)
                     /locus_tag="PC621_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4110193.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(7738..8214)
                     /locus_tag="PC621_003053"
     CDS             complement(7738..8214)
                     /locus_tag="PC621_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF4110194.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            8481..9656
                     /locus_tag="PC621_003054"
     CDS             8481..9656
                     /locus_tag="PC621_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF4110195.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(9737..10540)
                     /gene="proC"
                     /locus_tag="PC621_003055"
     CDS             complement(9737..10540)
                     /gene="proC"
                     /locus_tag="PC621_003055"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF4110196.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(10580..11164)
                     /locus_tag="PC621_003056"
     CDS             complement(10580..11164)
                     /locus_tag="PC621_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110197.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         3914 a         1893 c         1298 g         4607 t
ORIGIN      
        1 ttttcttaag aaacagttat tccttactaa tgcaatatac ctatttttac taatataagt
       61 ttattaatta gcattatact aatttatctt aatatgattt tttgttgatt ttaagctgtt
      121 tatcgtataa ataaaatttt tcttgactat atagttacta tatagcttat tatagataga
      181 gagtaataaa aggaggtaaa atttatggaa aatttattta caagaaaatt cactactttt
      241 gaatttctaa aatttgtttc tcctgcaatt atatccatga tatttatatc tttgtacaca
      301 ataatagatg gcatctttgt atcaacatta gttggttccg atgctcttgc tagtataaat
      361 attgtactac ctataattaa ccttgtttgt ggatttggaa taatgatggc aacgggtgga
      421 ggtgctatcg tttctatacg tatgggtgaa aatagacagg atgaagccaa ctctacattt
      481 tcttttatag ttttgttttc attgattgtt gggatttcat tcacagtaat ctcatatttc
      541 tttatcaaag aaatatctat tttgcttggt gcaacagata agttattacc atattgtata
      601 acttatggta aagttatgat tttatgtaca ccattttata ttttaaaatt tatatttgag
      661 tactttgcaa gaactgatgg aaattctaaa tttagtttat ttctatcagt cattggtggt
      721 gtaacaaata taatattgga ttatgtattt attaaatatt ttggaatggg tcttttagga
      781 gctgcagttg caactgctat aggtattatt ttaacttgtg ttttaggtat tatttacttc
      841 ttatctaata aatctacact caaattaaga aaaccaaaaa ccgattttag acttataaga
      901 gatactatga taaacggttc ttctgaaatg gttacagaat tatctacagg aattacaaca
      961 ttcttattta atgtagtagc tttaaaatta gcaggagaaa atggacttgc tgctcttact
     1021 atagtattgt atgctcattt tttaatgaca tcagtctatc taggattcgc tgctggagtg
     1081 tctccattaa taagctataa ttttggtgct gaaaacagtg ataaattaaa agaaacattt
     1141 aaacattctc taaaatttat atttgtttct tctcttttag tgtttattat tgctttagta
     1201 tttgcaccat ttattgttag ggtttttgta agtccagata acacagtatt taaactagcc
     1261 ttacaaggat taaaaatatt tgcatttgct tttttgtttg ttggtataaa tatatttgca
     1321 tcaggatttt ttacagcatt tcacaatgga aaaatttcag ctattatatc ttttagtcgt
     1381 gcctttgttt ttataatcat aggaatcata attcttcctc ctatgttaaa catgactgga
     1441 ttatggctta cagttccatt tgctgaggtt ataaccatat ttatatctat tctatttata
     1501 aaaaaatata aaggtagata taagtattaa aaattaatgc ttaaaatcaa catttattaa
     1561 aactaataaa cctgctttaa aatacaaatt tacaaatttg atttttaagc aggttttatt
     1621 ttagttataa taagtcattt gcatactctt taattggtgc ttttctaagt aataaaatta
     1681 taaatattat gcaaataact ataattccta cctttgttcc aaataaaata aaaggattct
     1741 gaaatgaata aataattttt tctaaataca atagcatttt atgcatattt ttagttgcaa
     1801 aattaactat ataaattacc attaatatcg gtaatcctat caatatagta atactagtaa
     1861 cttttctaac tctatatgta aaagctccaa gtaatactcc tataaaacct atacatattt
     1921 catttataca tatttgtaca aaataattta aataagttag tggcatactt gatgttgaag
     1981 gatctatcag tgctgatata gatgtccaaa tcatatctct ctttagtaaa actgatagtt
     2041 ctatactgta acctgttata atttctataa ataacttcga taaaaaaatc aaaactatac
     2101 ctaaaataga aatcacaaag cttaaaataa ttaaatctat gacaatagct tttatacaac
     2161 tttttcgatc tgctcttata cttaaagctc ctgaaaagtc tttatttgct atatttactc
     2221 catacacaaa tagcagtata gttatatatg ttaaaatagg attcacaatc gtggataaat
     2281 tagttttaga tgcttctcca aaaatcttga ggataaacac tggcaaaata ttcataacaa
     2341 ttcccacaat cataaatata attatataca atttagtatt tttattaaaa aattttatat
     2401 atggctttaa ttcattcaat tacaattcct ccttcttagt catgttaaca aacatatctt
     2461 gtagccctat gtatcctaca tcaatatttg aatttttaag tattttttca tcattttcac
     2521 taaaatcacc atagtaaaaa taagcaactg catttccaaa tgccttttta ggttttatat
     2581 tttttataca ttctaatttt tctaactctt ctttatttcc agtaatataa tgagcttttt
     2641 gttttatgac atcaatttct tcatctataa taatttttcc ctcatttaat attattacat
     2701 gctccagtaa atcatctatt tcatcaatca aatgagttga tattattatt gtccttggat
     2761 tttttatata actatctaaa attatgttat aaaactcttg tctatttact gcatccaaac
     2821 caattgttgg ctcatcaaat atggtgatag ctgaattaga acaaatacct attatatttg
     2881 aaagtatagt tttcatacct cttgacagtt gtttatactt ttttttagta tttaaatcaa
     2941 aatatttgca taattcttct tctaagcctt tatcataact tttataaaaa ctagaagaaa
     3001 attcaaatat ctgacctact ttaaaatctg gactataaaa ttctttttcc ctgacgatac
     3061 aaacttcttc caaaactttt atatcttctt ttgggttctt tcctaatatt tctatctcac
     3121 cttcattttg aattaactga tttaccatta tatttaataa tgttgttttt ccagctccat
     3181 tcttacctaa taaaccatat atcttatttt tttcaaagtt tagagacata ttattaaaaa
     3241 tattttgctt cttaaaactg tgagataaat tttctatctt aatagaactc ctcatatatt
     3301 aatcctcctt gaaattaatt ataatatcaa ccaattcttc tctatttatt tctaatttgt
     3361 tagcttcttg aagtaggttt tttacaaaat tatgtttaaa attttctttt ctagcttctt
     3421 ttataatagt ttttgcacca tctgatacaa acatccctat ccccctcttc ttatataaaa
     3481 tatttttatc aaccaatata tttatacctt ttaatactgt agctggatta attttataaa
     3541 gctttgaaag ctctgttgtt gatggaacct gttcttcttc ttttattcca tttgatagta
     3601 tctcatcttc aattgctcgt gctatctgaa taaatatagg ttcttcatta tttaatacta
     3661 atttcatagg ttcctccttt ctccattagt tagttactca actaattaac tatataactt
     3721 ataatatctt gaaaaatatt ttctgtcaat actaaataaa aaacagaggg aaaattccct
     3781 ctgttttttg ttctttatat ttataattaa attttgattt ttgtaatcct atcccataag
     3841 ttaaaaactt taataatctg ttgtatcttc attttttaat attttacata ttgagctagt
     3901 tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg atttaactcc
     3961 ccctgcagct tttattttta cattttttcc aacatgctct ttcataagct ttatgtcttc
     4021 taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg aacctgacat
     4081 tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg tctctataat
     4141 gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt ttcctatatt
     4201 atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt catctgctcc
     4261 attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat atcccaaagg
     4321 gaaccctatc actgtacata tctttatttt accttttaga tattctgatg ctcttttcac
     4381 atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat cacataaaat
     4441 tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg tttttaatat
     4501 atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt ttttaaatgc
     4561 aattttctct ttgcaatatt cagtatcata tcaacctctt caactttaaa aatagtcatt
     4621 agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac aaactcatta
     4681 aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga taaaacaccc
     4741 ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc ttgtccaaaa
     4801 tatccaactt ttctctttag gttcaagaag agtaacaata tacatgcaat tgatgatgaa
     4861 ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa gtccaatact
     4921 atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc ttgtaatgaa
     4981 taaaaaacct ttcccaaaat atctcttaat ccaaatgcag tcataccaac tgcataaaaa
     5041 attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct ttcaaaaatg
     5101 attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat catagaaatt
     5161 attatcatat ttatactaga tttaactgaa ctagtaaact tcttttgatt attttctgca
     5221 cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa tgtccctgta
     5281 acaaaaccat ttaatctatc tgaataagta agtgctggaa tgcttccttt aactaatgtt
     5341 gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc tataaatact
     5401 ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa gtagtataag
     5461 tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac taccatagca
     5521 accacagcac caattggaag tatgtatggt ccaaatacgg tacttaacat tattgaaata
     5581 attattacta tattataggg tatactccca aatccaacta caataaagtt ctctttaatt
     5641 tgcagaaatg ctgacataac actcgtaata cctatgaata ttattcctgt tatcaatatt
     5701 ttagtaaatt ttacggtaag taaaaacctc tctccttcaa atcctatagc aaatatactt
     5761 acaagttgct ttgagaaaat aactcctaat atagctacaa ctatgcatat accaactatt
     5821 atatttaaaa cattgtttaa aaactttagt gcttgctttt caccttgttt actacttata
     5881 tcttgataca ttggaataaa agtagtaact atagcagtac ctattgctgc aaatataata
     5941 tttggtatat tcatagctgt taaataactc tctgtatata agcctgtacc ataaattgat
     6001 gataatacta attctctacc cattccaaga atcttagaga ttatagtaac tatcatcaaa
     6061 taaaaagttg ctttagctac cttgctcatt ttatcactcc acttctaaca tacctctttt
     6121 taatcaagta aaatttggct cattagatat aattctatca actaatttgc cttgcttagc
     6181 cctatcaaaa tataattaac acctatttct taatatatca caaactacat tattcttcat
     6241 atttttttat ttctccaata agttttacat ctttcctata taaattgtat agcttcaatt
     6301 atattcaaag aaatggagtt gctacccgat aactttaaaa gtaaaaaaca gattttatta
     6361 tctttttaaa ctatattcta acatattttg tccatattga gaaattttac taaactatta
     6421 tgtactttat taaataaata aaataacagc acacctataa aatacaagtg tgctgttcat
     6481 aaacagattt tataaaaaaa taattcctaa gtagagatta acatgctttc tcatactctc
     6541 atatataatg tgtctaaaaa tattaataaa aatgctttaa ttgttttaac ttacttggat
     6601 gtcttaattt tcttattgct ttagcttcta tctgtcttat tcgttctctg gtaactccaa
     6661 atacctttcc aatctgttct agtgttttag ggtcatcatc atcaattcca aatctcattc
     6721 ttaaaacttg ctgttcttgc tcaccaagac ctgtaagtag ttcttctatc ttttctttta
     6781 aattatgctg ttcaacttcg tgtattacaa catctttaaa atcagcatct gatggtataa
     6841 attcaactaa actactatcc tcatcttctt ttaatggagt atccaaagat acaacatttt
     6901 tatctcttct taataattcc aacactttgt caacttctaa gccacatgca tctgcaatct
     6961 catctatagt tggctctctt aataatacat ttaaaagttc ttgtttcttt ttctttacaa
     7021 aatttattct ctgatgaaga tgtataggta tcctaatagt attttcgcaa tcgtcgatat
     7081 atcttgttat actttgtttt atccaccaag ttgcataagt gctaaatttg tatccttttt
     7141 tataatcgta cttttctaca gcttttataa gtcctatatt tcctgcttga attaaatcca
     7201 acatatcaat actatctctt ttatatctct ttgctatgct cactaccaac ctataatttg
     7261 aatttataaa tttttctttt gctactgaag atgagttgat tatttcctta gccagctcta
     7321 cctcttctcc tgctgacaac attttgtatt cctctatttc ctttaaatac attttcatcg
     7381 catttgatac atttgatgaa cccacacaaa tataattttc ataattattt tcttttttag
     7441 tatccataat caaacccctt ctattcatag aatttgctaa aacaaaaata aataatttaa
     7501 acctacataa tctacaatta cataatatcc cattctttaa atgaattgtt ttgtatatta
     7561 tgtatacaaa tttataaaaa ctatacctat aatattattc tacttctaac cgcaatatcc
     7621 tccttatata tataatttat tttattatat gttgttatat tcttagtttt ttgaatgata
     7681 tatgtctaaa aaaagactgt ttactaaaca gtcttttttt atatatatgc tcaatattta
     7741 attttcatga tgatgattac atccacatcc acaattttta tcatggtgat gatgttcttc
     7801 taaatctttt gcctgttgtt ccatttcatg aaactctttt accattatat cataagttgg
     7861 aagagctgcc tcagtatcgt attcttcccc tctccaatca gcgtacatca tagcatcccc
     7921 attagttaaa accaatctac ctgacatagt tatattatcc acttcttcta aaaattctat
     7981 ttgtttttgt aaaacttctc cttctaactc tacatcttca tctaaacatt gcactatcat
     8041 tacaggtgca taataatctt cttcatcttt aacatttaca acaatatcta aaactccttc
     8101 tgcaccttct tcaaattctg ccaactcaac atttgtatca atcatatctt gagggactaa
     8161 taaatcttct attaaatatt ttattactct atcttttact aattcttttg acatttattt
     8221 ttctccttcc gattctataa cattatcata atcatttcta tattatagta tactacatta
     8281 attgtttttt caatctctta caaaaattat ttttttatta tcttccttat attccctttt
     8341 ttattacaaa gttgtaatcg cattgattta aaaaatagtt tatatttagt ataatagaaa
     8401 tgtagcacat atttttattc aatatattaa taaaaaaata gattgaaaat gtaatgtatg
     8461 ttaaatctag gaggaatatt atgaagttta aaaaacaaat tagttctatt gttgtgtcta
     8521 ctatgcttgt acttgggagt atgaaccttt cttatgcaga tggtacaaat gtagttacac
     8581 ttggagctaa cttatcagag tctcaaaagc agcaaatgct taattacttt ggagtaaaaa
     8641 aagaccaagt cttagtttta gatgtaacaa atgcagaaga aagacagtat ttacaaggtg
     8701 ttgcaacaga gggtcaactt ggaaaagtta ctatctcttg ctcatacgtt gaacctacta
     8761 aaaaaggaaa tggaattaat gttaaaacag caaacttaac ttgggtaact agttcaatga
     8821 ttgcttcaac ccttacaaca gctggtcttg aagatgcaaa tgttattgct gctgctccat
     8881 atcctgtaag tggtactgga gcattgacag gaatcatgaa agcatttgag gatgcttcag
     8941 gaaagaaact tgatgaaact aagaaagaaa ttgcttctga agaattggta gttacaggtg
     9001 acttaggaga tgatataggg caagaaaaag caacaggtgt aatgaatgat ataaaaacag
     9061 aaattgtaaa gaatggtaca aaagatacca atcaaatagc aaatacaata aataatgttg
     9121 taaataatta caatattact attacaccag aacaaaaaga acaaattaaa ggtgtaatga
     9181 aaaaaatagc tgaccaaaat tatgattaca atagcatgaa aaatacacta gataatgtat
     9241 cagataatgt aaatgaacaa ttaaagaaat taggtgaaag tgttaagggt tctggtatac
     9301 tagatactat tggtggatgg tttagtggaa taggagactg gttctctggt ttattctcag
     9361 gcggagataa aaaagatttg ggaatcttaa ataacactaa tgatgagtct cttggctctg
     9421 atgctgtaat aaattctaca gaaggtgttc aaataaatcc acaaaataat actaattcta
     9481 acaatgatag tgatacaaat agctcaaaaa atgatacttc aagtaataat gaaaataacg
     9541 atagttctca aacaaatgaa aacaacacaa caaataattc agaaaatcaa tcaaataata
     9601 atgactcagt tggtttcttt gaaaaaataa aaaattggct ttcaggctta ttttagaaat
     9661 taaacaaata aaaagaattg cctaaaatgg aaattttcta taaaaggcaa ttctctttat
     9721 ttacaataaa tttttattac tcactcattt ttttagattt ttctatacat gcacatattg
     9781 cttttatcac agaagctcta aaaccttctt cttcaagtac tttaactgct tctatagtag
     9841 ttccaccagg tgaacatacc atatctttta attcaccagg atgttttcct gtctcaagta
     9901 ccatttttgc agaacccata acagcctgtg atgcaaactt atatgcttgt ggtcttggca
     9961 ttcctgcaat tactgctgca tctgctatag cttctataaa taaaaataca tatgctggtg
    10021 cagaaccact tgcaccaatt acagcttcta taagatactc tggtactacc tcagtatttc
    10081 caaaagaatt aaatacttct gtaactgctt ctaagtcttc attgtttata tttttattta
    10141 tagaaagaga agacatggct tcatttacaa gagctggagt atttggcata gttctaacta
    10201 tttttttatc ttctcctata attgactcta tatcctttat agattttcct gctgctattg
    10261 taactataat tttattgtca tctataaagt cttttatctc ttccaaaata tcatcatata
    10321 tatctggttt aacagctaca attactatat cagaggttct agttacttcc ttcgagtctt
    10381 ttgtagtatt tattccaaaa tttgcatcta ttctatcgag agttccttga ctatatgctg
    10441 atgctataac catattaggt tcaactaatt ttgaatttac tattccccct atcatagcgc
    10501 ttgccatatt tcctgcacct atgaatccaa tttttttcac tttagtacct ccttattgac
    10561 taatagtaat cgttaaaatt taagcttctt taactgcatc agacttttca aatggatttg
    10621 gtatacttac taccaaaatt ttcactacct tattagtata gttgacccaa ttatgattgt
    10681 tcttttcact attatagtgt attgtgtctc ctggatacat ttctatttgt tcatctccca
    10741 aaaacactgt aagagtaccc tctaatacat acacaaattc ctcaccttca tgtacgtaac
    10801 aacaaatttc ctcactactc ttacttggaa gaatttcaat taatcttggt agcattgttt
    10861 tttcctttaa actagatgat aaatgataat gtataaatgt tgagttttct acatcaaata
    10921 cttctttttc ataacttctt aatacagctc ttttatgctc ttttggcttc ataaaaaagt
    10981 aagttaattc tacatctagt acacttgcta tttttcctaa ggaatctgtt gccacacttg
    11041 ttaatcctct ttcaagttgt gataaaaaac caattgacaa gtttgttttt tcactcatat
    11101 ccttcagcgt catttgcttt tgagttctga gttgctttat ttttgctcct atgtccttat
    11161 tcaaaaccat cacccctaaa taattttaat tttttcataa tataaataca aaaatataaa
    11221 tataggtact gaacctatct ttatttcatt taaaaaaaat ttttataagt attactataa
    11281 tttatcaatc tttttttgtc tacattttta cgattattta cgatttattt ttcacatatg
    11341 tatatttaat cttgccaact aaagatacat tttttataag catttactct ttatataata
    11401 aaacttaagt tgttcatgta gtcttcttct ggtttaatta ttaaaatttc aaattatatt
    11461 attatttcta cacattatta catcctttag tacaattata acataatttt acaatttaat
    11521 gatatctatt caatgtcttt ttttactcta caacatattt tattttagag tttaatacta
    11581 tatatatttt ttatatacag ccttgtcatt ttttagattg tcaagagtta ttgaagtatt
    11641 ttatgtagat ttatatttta ttttctaatt tctcatttaa caagaattgc ttatgataaa
    11701 aaataatatc at
//
LOCUS       DAMKPG010000104        11402 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMKPG010000104 DAMKPG010000000
VERSION     DAMKPG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.109"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     regulatory      204..247
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            348..1316
                     /locus_tag="PC621_003057"
     CDS             348..1316
                     /locus_tag="PC621_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF4110198.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1318..1881
                     /locus_tag="PC621_003058"
     CDS             1318..1881
                     /locus_tag="PC621_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF4110199.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1889..2578
                     /locus_tag="PC621_003059"
     CDS             1889..2578
                     /locus_tag="PC621_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4110200.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2705..3433
                     /locus_tag="PC621_003060"
     CDS             2705..3433
                     /locus_tag="PC621_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF4110201.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3447..4121
                     /locus_tag="PC621_003061"
     CDS             3447..4121
                     /locus_tag="PC621_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF4110202.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4147..4983
                     /locus_tag="PC621_003062"
     CDS             4147..4983
                     /locus_tag="PC621_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110203.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            4999..5670
                     /locus_tag="PC621_003063"
     CDS             4999..5670
                     /locus_tag="PC621_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110204.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5660..6892
                     /locus_tag="PC621_003064"
     CDS             5660..6892
                     /locus_tag="PC621_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110205.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            7042..7359
                     /gene="trxA"
                     /locus_tag="PC621_003065"
     CDS             7042..7359
                     /gene="trxA"
                     /locus_tag="PC621_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF4110206.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7376..8254
                     /locus_tag="PC621_003066"
     CDS             7376..8254
                     /locus_tag="PC621_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF4110207.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            8524..8892
                     /locus_tag="PC621_003067"
     CDS             8524..8892
                     /locus_tag="PC621_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4110208.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            8895..9266
                     /locus_tag="PC621_003068"
     CDS             8895..9266
                     /locus_tag="PC621_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF4110209.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(9410..9865)
                     /locus_tag="PC621_003069"
     CDS             complement(9410..9865)
                     /locus_tag="PC621_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110210.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(9904..11169)
                     /locus_tag="PC621_003070"
     CDS             complement(9904..11169)
                     /locus_tag="PC621_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF4110211.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         4586 a         1192 c         1895 g         3729 t
ORIGIN      
        1 taataatcgg tttaaatgcg ccacatctta ttaaagttgg ttcataattc taaataaact
       61 ttaataagat acattaaaat ataataaatt cttttatgct ataaaattta ttttatagct
      121 attaaatcag atgtttaaaa attttttaac agtctatagt ttttaagagt ctatagtaag
      181 tagcctaaaa atatgatttt acatctgtac ttgttcgaga actccaagta taaaaaacta
      241 aggaattata tagtggcagt atataattaa tacccttaga cacacctttt tgaaagtgga
      301 aaaaagatgt gtagttctcc tcgaccacaa atcaaggagg ataaaaaatg gaaaagagaa
      361 aagtaataat tgattgtgac ccaggaattg atgattcttt ggcaattctt ctggctttaa
      421 actcaccaga gctagaagta attggaatta ccacatgttg tggaaatgtt ccagcaaata
      481 taggtgcaga aaatgcacta aaaacacttc aaatgtgttc ttcactaaat attccagtat
      541 atataggaga agaagcacca ctaaaaagaa aacttgtaac agctcaagat acacatggag
      601 aagatggtat tggagaaaac ttttatcaaa aggttgtagg agctaaagca aaaaatggag
      661 cagtggattt tataataaat actttacata atcatgaaaa agtatcaata atagcacttg
      721 caccacttac aaatatagct aaagcactta ttaaagataa gaaagcattt gaaaatctcg
      781 atgagtttgt atctatggga ggagcattta ggattcatgg aaattgctct ccagtagcag
      841 agtttaatta ttgggtagac ccacatggag cagattatgt ttacaagaat ttatctaaaa
      901 aaatccacat ggtaggttta gatgtaacta gaaaaattgt acttactcct aatattattg
      961 agtttataaa tagacttgat aagaagatgg caaagtatat aactgaaata actagatttt
     1021 atattgattt ccattgggaa caggaaggaa taattggctg tgtgataaat gaccctctag
     1081 cagtagcgta ctttatagac agaagtatat gtaaaggatt tgaatcatat gtagaagttg
     1141 tagaagatgg aatagctatg ggtcagtcta tagtggattc tttcaatttc tataaaaaaa
     1201 atcctaatgc aattgttcta aatgaagttg atgagaagaa atttatgtac atgtttttaa
     1261 agaggctttt taaaggttat gaagacatta tagactctgt ggaaggagtg atatagtgtg
     1321 aaaagaaaaa tatgtgtaaa aactattaca cttatagctt ttggtatttc tataaatata
     1381 ataggcgctt ttatagctat gggattaaga ttacctgtat acttagattc aataggaact
     1441 ataatgatag cttcactttt aggacctaaa tacgctgttg ttacaggtgt gtttggaagt
     1501 ttaataagtg gaattacgtt tgatgtgtat tctctctatt ttgcaccagt tcaaatatca
     1561 accggattac ttgctggaat agcatttaaa aagaattttt taaggggttt aaaaactcca
     1621 ctaggagtac ttttatttgc tattccaaca tctataataa gttcaataat ttcagcattt
     1681 ttatttggag gtatgacctc ttcaggttca tcctatatag tgcaaatctt gaaagtgtta
     1741 ggactgggag atgtttttag tgtgtttgta acccaagtat ttactgatta tggagataag
     1801 ctgttagcag ttgttttagt gaatttagga ttaaatgcag ttccaaaaac tttaaaggta
     1861 tcattgactg gaggaaaata gatagaaaat ggatagatat agtgagataa caaataaaaa
     1921 tcaaagagaa attgttctgc ttaaaggatt tccttgtata tggggaaagt gttctttttg
     1981 tgattatata gatgataatt ctaatttaga agaagagatg aataaattaa atctaagggt
     2041 tttaaaaaat gtaactggga aatatggagt tcttgaagtc ataaattctg gaagctgttt
     2101 tgaattacct aaggatactt tagaaaaaat aaagtgtatt ataaaagaga agaatataaa
     2161 aaaattattt ttagaaagtc attggtctta caaaaatagg cttaaggaaa tgagagagta
     2221 ttttgaaatt cctgtggtat ttaaaattgg agttgaaacc tttgacaatg attttagaaa
     2281 taatatttta aataaaaatg caaattttaa gactccacaa gatgtaaaag aatattttga
     2341 ttcaccttgt attatgattg gaataaaagg acaaacaaaa gagatgatag ataaagatat
     2401 agagattatc ctaaatactt ttgaaaaagc gacagtgaat gtttttataa ataattcttc
     2461 tagtataaaa agagatgaag agttagtgaa atggtttgtt agtaagtata aattcttgga
     2521 tgaaaatcca aatatagaag ttttatacaa taatacagac tttggtgtag gagattagca
     2581 ttaatttagt ttaaatttaa aattgaaaag tttcttatgt ttttgaaaaa gcttctaata
     2641 gttagaagct ttttttgtag agttttatgt tacaataaga aaaaaggaat taaggagcaa
     2701 ttaaatgatg tttgataggg gaaaatggaa taaattggat tataatgaac ttttagaata
     2761 tatgatgtct ataaaagata taaaatatag agattttaat aaaaagctta tacctggaac
     2821 agaaaatata ataggtatta gagttcctaa tttaagaaag ttatcaaaag aaataagcca
     2881 agggaattgg aaggaatttt tagaagtagc agaggataca tactatgaag aagttaggct
     2941 acaaggtatg gtaattggca atataaatag taattttgaa gaaactttat actatgtaaa
     3001 aagatttata cctaaaatcg ataattggtc agtttgcgat gggttttgtt ctggtttaaa
     3061 gtctatgaaa aaatataaag aaaatatgta tgatatactt aaaaaatatg tttattcaaa
     3121 gaatccatgg gaaattagat ttgcattagt tatgttttta atatattatg ttgatgataa
     3181 acatattaaa gaaatatttg aatactgtaa taatatacaa agtgaagaat attatgttaa
     3241 aatgggtatg gcatggttgc tatcaatttg ttttattaaa tgtgaagagg aaacttttct
     3301 ctatataaaa aataataatt tagatgattt cacttataat aaaatgctac aaaaaataat
     3361 tgaatcaaat agagtagatt tagaaaagaa gaatataata agaagtatga aaagaaagac
     3421 aagaagatgt tgacggagga atattaatga ataacaatga agacagaatt gacaaatttt
     3481 tagaacttca aagcaaagga tttaaatttg cagagatagc tcaaaatcta aagattagtc
     3541 aaagtacttt aagaactttt ttaaataaaa aaggatataa gtcagaaaaa ggtaaataca
     3601 tattaaaaga cgaattaaat tataataaaa gtttggataa tagaaacttg gaagaagaga
     3661 aagctaagga gtatgaaaaa agtaaaaaga atactataag taatagaaaa aaagtaagca
     3721 ccaaaaaaaa gatttctaat gaaaaggctt taagtaaaag tgttgaacaa attgagttta
     3781 atgatacagg tttaaaaaat gcaaagcgaa aacaaaatgt taagcctaag aaagatagaa
     3841 aaataaatat aactcaagaa gatttagata agctttgtga agtatatgat tggtatttag
     3901 aagtaaaaga ttacaatata aagcctaaaa aagtatctaa taaaaaagat gtacatatag
     3961 atgatgaaca catagatgaa ttaaaaacaa caagaatacg tatagataaa gaaatatgga
     4021 atgattttga aagattatgt agtaattcga attttgaaaa acatcaaata cttacacagg
     4081 cattgaaaga atttatgaaa aagtataaaa atctactata aaatactact atatgaggtg
     4141 cttaatatga ataaaatagt aaaaaaatca atttctctaa tgattatatt aacaatattt
     4201 atttttatgt tgacagcatg tgaaaaagat gaacagcctg attcagttga tattcaaaca
     4261 gaagataaaa atgagataaa aatagatgag ggaaatgcaa aagtattgaa tataggaaaa
     4321 tcagaaatta taaatataga tgaaggtgat aaagtagata catcaacaaa ggtagaaaat
     4381 aattcaacat ttaatatatc caatgttgaa ttgatatata atgaatatga tgagaataaa
     4441 aaaattatat cttcggatag taaggctttg ttggatatga ctttaatgcc tgggaaagtt
     4501 gcttacatag aatgtgggca taaaactttt gtaaagggtg tagaagttta tgcatatgaa
     4561 tatgaggcag agggtaagat tgtgtatgtt aatttaaaag aaaatactat taatataaga
     4621 aataataata ttaagctaga aaatagtagt cagtatgagg tgctatctac atcagaactt
     4681 aaaaaagtaa atgaatcaaa tgagggaatt acgtatcaag taaaagtaaa aaattcatca
     4741 tcaaaagatt taggaaatat tattcttaag acagcagaag ttaatgaaaa tggagaatat
     4801 ttaacagtaa atcgtgtacc atcatataag attttaaagc cttcagaaga gacagatata
     4861 gatatattgt gttcaacaaa ggcaaaaagt gttgaaatag tgggatatac gtatgatgat
     4921 ataaaagaaa aagccaatgt ggatattgac ttaaaatctc ataaagttaa gatagacaaa
     4981 taaaggtagg ggatacttat gaaggtttta atagttgaag ataataagat acttttagaa
     5041 agtgtagtag aagaacttag caagcatttt gaaactgaaa agtgtgaaga tggagaagaa
     5101 gctttatact tagtaaatca aaatatatat gatttagtta ttttagactt aatgcttcct
     5161 aatataaatg gatttgatat cctaaagaaa atgagagtta ataatataga tacacctgta
     5221 cttatattga cagcaaaaga aacattagat gacaaagtag aagcatttac tataggagct
     5281 aatgattatt tgacaaaacc tttttatatg gaagagttag tagctagggt atacgctata
     5341 ttaaggacta atggaaagat aaaagaaaga aacggattag aatttaaatc tttatattta
     5401 gatacactag aaaaaagagt gtatattgaa aaagaagaaa taaaacttca aaacaaacag
     5461 tttaacttgc ttgagtattt tgtattaaat aaaggttcaa ttttattaaa agagcagatt
     5521 tatgatagaa tatggggaat tgattcagat gcaactattg aaatagtgga agtatatgta
     5581 agtaatctaa gaaaaaagct aagtaaatat ggatatgata aatatattaa aactaaacgt
     5641 aaggtaggat atatattcga tgataaataa gaatgtattt accagtacta aaaatcattt
     5701 aataaaaatg tatattattg tagtaggtag ttttttaata atcttttcta tatttatata
     5761 ctcatatttt aggggactta catatagtgg aattgatagt gagataaatg atgaacttga
     5821 atatatagtc tctcaattta aaagaacttc atttttgaac ccaataagac tgaaagatcc
     5881 taaagatatg gtgtatgtat atgaagatgg aaggataagt tactatactc aaaatgaata
     5941 ttttgatgag ttacttccag atagaagact agataaaaaa aattcgtttt ttaaatacac
     6001 agaaaatgga tatactttta gagaattaaa tgtagatgta ggtagatacc aaatacagat
     6061 aattagaaat atagattctg agatgaattc tttaagacag cttacatctg ttcttataat
     6121 tggaatatta atttctgtaa taattactta ctttgtggct gtatatctca ctagaaaagc
     6181 tttaattcca atagaaacag catggaaaaa tcaagctaag tttatacaag atgcttctca
     6241 tgaattaaga acaccaataa caatagtttc atcaaaatta gaaagtatgt taaaatcccc
     6301 agaaagtaca gtaaatgatg aagttgaaac aatagctact gcaatgaagg aaactagaag
     6361 acttaaaaaa atgataactg atttattgtc tcttactaaa gaggactcta ttgttaaagt
     6421 taatttagaa gaaatagact tagaaaaatt attggaagaa atatcagaag actatattga
     6481 cattgctgag tttcaggaaa aaagattcgt gtttaattct aaactgaaga ataaagttat
     6541 aataacagat aaaaataaat taagacagct tatattaatt ttcattgata atgcctttaa
     6601 atatacaaaa ttgggtgatg agatagcatt agaattaaaa gaagatatag aggatgaggt
     6661 aactttactt ataagtgaca ctggtatagg cataaaaaaa gaagagatac ctcttatatt
     6721 tgatagattt tttagaagtg aaaatgtaag aaataaagat ttagaaggtt ctggaatagg
     6781 actttcaata gctcgcatga ttagtttaaa cttatctatt gatataaatg taacttctga
     6841 tgtagatatt ggaactacat ttgaattgtc aataccaaaa aagttaaaat aataaaaata
     6901 taaaaaattg tttttagaaa tgaagaatgg gtatactaat tagtataaaa tataaaaaga
     6961 ccttataaaa gtaaatttat atttataaat ttcatagtat aattatatag ggataataaa
     7021 gtttaatagg aggatttaaa aatggcaaaa gttataaata catctgaatt tagaagtagt
     7081 gttgaaggta gtaagggagt agttgtagtt gatttctttg caacttggtg tggaccttgt
     7141 aacatgttgg gacctgtatt tgcagaatta ggtgaagaga tgaaggataa agctagattt
     7201 gtaaaagttg atatagatga gagcttagaa atagctcaac aattcaatgt atctacagta
     7261 ccaactatga ttatatttaa agatggaaaa ccagtagaaa ctttaattgg attcatgcca
     7321 aagaacaaaa ttgaaatgca agtaaaatca tatttataaa aaatgggtga gtattatgag
     7381 atatgatata gctataatcg gcagtggtcc agctggattg tctgctgcta taaatgcaaa
     7441 aataagaaat aaaacaatta tcatgtttgg taatgataat ttaagtaata agcttgttaa
     7501 agctccttca atagataatt atttaggttt ttatgatata agtggtgatg aattaaaaga
     7561 caagtttaaa tcacatatag atagtatgga tatttcaatt gaaaataaaa gaataaataa
     7621 tatttatgct atgggagaat actttacaat aatgtctgga aatgatatgt atgaagctac
     7681 tactgtaatc cttgcaactg gggtagagta tactagacca atcaagggtg aagaagaatt
     7741 tctaggaaga ggtgtaggct actgtgctac atgtgatgca ccactttata gaaataaaaa
     7801 ggtagcagtt ataggatata atgaagaatc taaagaagaa gcaaattttt taagtgaact
     7861 cacttcaaag acttatttta tacctatgta caaaaaagat aatcttatga gaagttcaga
     7921 taacctagat gattctattg aagttataca tgacagacca gtacagatag atggagataa
     7981 acttgtaaac aaagtatctt ttaaagaaaa ccacattgaa gttgatggag tattcgttat
     8041 aaaagatagt acggctccaa gtgcattggt tcctggaata gagattgatg gcattcatat
     8101 taaagttgac aataatatga agactagtat agatggatgc tttgctgctg gagattgtgt
     8161 tggaaaaccc tattcttata taaaagcagc tggtcaaggg caaatagcag ctttaaatgc
     8221 tgtttactat ttagataaat taaagagggc ttaatattta tattgattaa ctttttaaag
     8281 atatttataa aaaactgtct tgaaataatt ttattatttc aagacagttt tttattttta
     8341 agttattaat aaaagtattt taaaatctat atgtaattac ttttattaat aaaattcaaa
     8401 gttatattag tagaaattgg aattatttat aatatactcc aaaatttaaa aataatggtt
     8461 gaaaaatcta attaagagta ctataattta attaagtaat tacttaatca aaacgaggta
     8521 cttatggaaa acttggttaa aatatttaaa gcattgagtg atgaaactag acttaacata
     8581 cttatattag tatccaagag aaatatatgt caaaagggga tttctaaata tctagggatt
     8641 tcagattctg ctgtttcaca acatataaaa atattaaaag atgttgggat aataacagga
     8701 tataaagaag gatactatgt tttatatcat attaataaag aatcatttaa tatatgtgta
     8761 gattttataa attctatgtt aagtaattct agtgaaagct ttatagatgt atttgatgta
     8821 aatacaatac atctaggatg cagtaaagaa tgtaaatcta taaaaaaatg ttgtaagaga
     8881 agggagaaat aataatgaaa gtatgtatac cagtagagga gaataaagga ttggatagta
     8941 aaccatatgg acattttggt tctgctccaa tttttgttgt gtgtgattta gaaagtggag
     9001 aagttaaatc tttagataat ggagatttag atcatgaaca tggaaaatgt caaccattaa
     9061 aagcattgtc agggacagct gtagatgcag tagttgttgg aggaatagga caaggagcta
     9121 taattaaatt aaatggaatg ggaataaaag tttacagagc agaaggtgat actataagtg
     9181 ctaatttaga cttacttaaa aatggaaaat tagtagagtt tccatcagac catacttgct
     9241 cacatgacgg atgtggacat cattaaaaaa aacagctgat gaaatatata cttcatcagc
     9301 tattttttct tttaataaat acgattttat tcttttaaaa ccaaaattta gatttttatg
     9361 ttttttgtag tatccttaat aatatttcaa gtttacaagt ttactacttt tagatttttt
     9421 ttgtatgttt ttctatatag tcaatatcat ttaatatgtc attgagttct ttattaagtg
     9481 tttttaaatc atcaccatct acattgtagt ttaaaaatag ttcctctaca ttatcaatga
     9541 tagaattgac ttttctagta acttcaacaa atgtcttaaa gttactacta tttttagcat
     9601 ttagaaattt ttttaaatcc tttgatataa atgatactat ttcatattca tcattaaata
     9661 gacctctacc aataggatat ggttcaattc tctgtaaaaa atattggtct ttagcattaa
     9721 tattataagt ataagatatt tcccaaccgt ctcctaaatc aaacttgata aaatataatg
     9781 tactacttac ttttgatatt cttgtggcac caagctgtct taaaagaact tcaatcgaat
     9841 caccttgaac agtattaata ttcatatata cacatcccct ttagtattat tatactatat
     9901 tttttaaaat tttataatta aataaacagt agatataata atagatataa tcataagtgg
     9961 aaaacctatt ttgaaatact ccttaaaaga gataggatga ccgtgtttat tacctatacc
    10021 tgctagtaca acattagcag atgctcctat tagtgttcca tttcctccaa gacaagctcc
    10081 aagagatgtt gcccaccata gtggcattac atcaatacct tcagcttgca tagtcaaaat
    10141 aagaggaatt agtgttgcaa caaatggaat attatctaag aaagaagaga ctatagctga
    10201 taaccataat atcaacaaca ttgtaaatac aagatgtcct tctgtaagtc caatcaatgc
    10261 ttgagccaat ttgtttatta tcccaacttc tactagtcca ccaactacta caaataatcc
    10321 cataaagaat aaaattgtgg accattcaat acctgccata atttcatcta catcttgttt
    10381 tcctattaaa agcattatag atgctcctgt cagtgctaca acagaagaat ctatgtggat
    10441 tgtactgtga aacataaaac caaaaagtat gaatgctatt aatataaggc tttttataag
    10501 tagaggttta tctttaacag attttttttc atctagtttt aatattgcat ttttagctct
    10561 ttcattcaca acaagttctt ttccataaat aaatctaaag catattatag ttatagcaag
    10621 tattactata acagcaggac ctaaatttat tacaaaatcc ataaaactaa gattggcagc
    10681 actaccaatc ataatatttg gagggtctcc tataagagta gctgttcctc ctatatttga
    10741 agctaaaatc tgtgttatta aaaatggaac tggattaagt cctaatattt gtgtaataac
    10801 tatagtcatt ggtccaataa gtaagacggt tgtaacatta tctaatacag ctgataaaat
    10861 agctgttata atagcaaaac aaatcattat cttccatggg tcacctttag ctttctttgc
    10921 agtccagata gctatatatt caaataaccc tgagtttttg acaacagcta caaatagcat
    10981 cataccaact aatacaccaa tagtattgaa atctatatgg cttaatcctt catcaagtgt
    11041 aagtatattg aatattatca ttaatagtgc accaccaaca gctgctacgg atctgtttac
    11101 cttttctgta ataatagctg ccataactat taaaaatata catatagcta ttatttgttg
    11161 aaattccata atataagtac ctccatttgt attattttgt gcctaatgct agtactatat
    11221 atagtattac ttttttattg ttttttcaat ggatgtatga aagtatatta tttaacaaag
    11281 tttttgagaa attgacagaa tttaaagaaa atttttgtta tttaaataaa ttttaaaatt
    11341 attccattga taataatatt aaacgacaaa cgaaaaaata taccctttaa actagaattt
    11401 at
//
LOCUS       DAMKPG010000105        11263 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMKPG010000105 DAMKPG010000000
VERSION     DAMKPG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.110"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(72..497)
                     /locus_tag="PC621_003071"
                     /pseudo
     CDS             complement(72..497)
                     /locus_tag="PC621_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(970..1257)
                     /locus_tag="PC621_003072"
     CDS             complement(970..1257)
                     /locus_tag="PC621_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110212.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1277..1552)
                     /locus_tag="PC621_003073"
     CDS             complement(1277..1552)
                     /locus_tag="PC621_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110213.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            1740..2559
                     /locus_tag="PC621_003074"
                     /pseudo
     CDS             1740..2559
                     /locus_tag="PC621_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(2818..5385)
                     /locus_tag="PC621_003075"
     CDS             complement(2818..5385)
                     /locus_tag="PC621_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF4110214.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5388..5993)
                     /locus_tag="PC621_003076"
     CDS             complement(5388..5993)
                     /locus_tag="PC621_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110215.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6367..7827)
                     /locus_tag="PC621_003077"
     CDS             complement(6367..7827)
                     /locus_tag="PC621_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF4110216.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8158..8736
                     /locus_tag="PC621_003078"
     CDS             8158..8736
                     /locus_tag="PC621_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110217.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9028..10278
                     /locus_tag="PC621_003079"
     CDS             9028..10278
                     /locus_tag="PC621_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4110218.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10439..11008
                     /locus_tag="PC621_003080"
     CDS             10439..11008
                     /locus_tag="PC621_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF4110219.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3713 a         1960 c         1583 g         4007 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcatga ctaaattcac gagtgtgcgt
       61 agctattttt taatcaatac aattactgca catatagatg cagaattttt aaatacttca
      121 cctaatgaaa ctgctgcact aatatcatgg gctactcccc tattgtcgct aaaacctgca
      181 gtatcacata attttgcatc cattccttct attggttttc tcatatttac aatgtttggg
      241 tataaagttt gagaaacagc tcctgaacca atctctgcca cagggtctaa gcaatcaata
      301 tatgatatac cagtatcttc atcttctact tccatcatta cacatcctgc aagatagtta
      361 attgcagtac ttttgccaca tcctgtccca ccaagaatta gaattaaatt ttgattttca
      421 gccttattta ttgctacttt tccttcattt aataagagtg ttatttcttc taaaagttga
      481 ttgttatttt ctggcattta ttttccccct tggttatata ttttatttaa ataaagttta
      541 atcccttaag tagattacaa agtttttaga ctatatttaa atttgttttt tatttttaat
      601 aaatttgcat tttcactcaa taattattct tctttgttta ttaaaataac tgtgttgtat
      661 aatataattt ttaccaacac agttatttta tttaatgcgc ttattttatt gtattttatg
      721 taatacagtt gtttttttat attatatgtc cactattttc ctatggttat tacatataga
      781 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
      841 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
      901 ataggtttcc tttagtttaa atagttcacc tatacctact tgaatagttc acttttattt
      961 gcttaaattc tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc
     1021 ttcaactaca ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa
     1081 aacattatca gttggtaata tttttacatc ataattattt aaattattat tcgtgatact
     1141 attaaataca tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc
     1201 ttcttttcta ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt
     1261 ccacctccta ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat
     1321 cacaaggaag aggtttaaaa acaattatct ctgctaaata atcatgtgga ggatttgtaa
     1381 ctgaagtagc attgcaaata aatttaaaat ttgttcctgc tacaacttga gtagcaacaa
     1441 taagtggttc ataatcaacc ccttttagca ttcctatagc ttcattaaat acttttaaat
     1501 cttcttcttc gacagcccta aattttgacc atccaccaac aaaaagactc atataaaaac
     1561 ctcctttata tatatattta tttatatgat agtaccaatt ttcttaatat tcaatatatt
     1621 acgtctaact ggtcaataaa aatgcataac tggataaaaa gacttataaa atagcttaac
     1681 tttacatata ataagattat tctaaaatat gagacatatc ttgctttgaa tatattctat
     1741 acaacaattt tccaggcctc attgccaagt ttaaggtaac tgactttgga ttccaacttc
     1801 ttgatgctaa taaaaaatat tttgatatgt tcaaagataa tgtaaatttt ctattagatg
     1861 atataaattt agaaggttat cttaacaatg tgtataaata tcataatgat tttaaatctg
     1921 ggagatgcgt ttgtttttct atttctccac aaactattaa tggagatatt tttcacatgc
     1981 aagttgtagc tgaatgtata gattgggaag atgataagcc tatatatctt ttaatatatt
     2041 cagatgtaac gacattggta aaacaatatg tctcattatc atttttcatc caagaatttt
     2101 atcaagaaat atgataaaat agctaaaaaa tatgatattc cttctcacct tatagaaatt
     2161 gagattacag aaacaattgt atttgaagat gtcaaagtat ttagtgaaat aataaatgaa
     2221 attcataaaa atgactttaa atgctctatt gatgattttg gaagtggcta ttcttcaatc
     2281 aatatgctta agggtattta tgttgataca ttaaaattag atagagcatt ttttcaatca
     2341 gaagatttag attctcaacg tgaaagaaat ataattgtat ctataatcga actagctaaa
     2401 aagttaaaaa tgaccactgt agctgagaga atagagacca gttttcaaaa agatttcttg
     2461 caagaaataa actgtgatat gttacaagga tacgtgttct ctagaccttt gcaaataaaa
     2521 gactttgaaa aacttatgtt tcaaaactcc tccaattaac aatataaaat tgcttattat
     2581 aagtgtatta ttgtatcaac aaataaagga attgtctcaa aattgatttt ttctaatcaa
     2641 atttttattt tgagacattc tcattttttt gcataagaaa atacccacca agctacggtt
     2701 tttcttaccg ctgttcggtg ggtattagta gtattatgaa atctgaaaac tttaagtatt
     2761 actacttccg ctaacgcaat cacaaaggtc gaaagcttca aagcgaaaat aggcttttta
     2821 ctcatcaaac gtcaccgtac ctgccaaccc gtccaccgtt atcatttgtc cgctcttaat
     2881 agttcgtgtg gcaatgtgtg tagccataac ggctgggatg ccatattcac gtgcgacaat
     2941 gcttgaatgg ctaagaggac caccaatgtc cgttacaatc gcacttgcag atgaaaataa
     3001 tggtgtccat gctggagttg tggtaactgc aaccaaaaca cttccaggtt gaaagctttc
     3061 aaaatctgct ggactattca gtacacaagc tggagccgtc acaacaccag tactggtacc
     3121 aatgcccgtc aacacaattt tcccatcttt ctgcttctgt ggtgcttgtg atatagtttt
     3181 tttgtttttc tctggcaact tggacggtga cacataaccc atgtattttt taagttcagc
     3241 ttttcgaaca ggtattatcc cgttcatatc agatagagac ttgtttttat ccaactgctc
     3301 tataagtacc tccaattccg attttgtgag ccaataaata tcatctaaat gtgaaatagc
     3361 tcctcctcgt atgaatcgtt ctgagatttc ctgaaacata caacgaatca gaggatgtcc
     3421 cattcccatc aaataaatag cattttcacg cataggagag gtttcctgtg cccaatggag
     3481 caacttaaaa aatagttttt tacgcaatcc acctatgtgt tgtaaaatct cttcttctgc
     3541 atgttttcta cgcttttcaa acgtggtctg gcgtaaaaaa ggactttccc cttttccttc
     3601 cacaaatgtt tttacggatt caaaagttgg tgtaagtgtt tcctgcggtg tggaatacgc
     3661 aaaatcaaac tcataagcgg tacgaccaaa ctctttaaag tacttattga ttcgattttt
     3721 ccattctatc catacttcct gtgaaactcc aacaggcata actgaagaca tgaaatcttc
     3781 tgctatcttt attgcaggac tgttttgcag ataaaggttg atgctgttat tttgctttac
     3841 ccattctgaa agaccccaca actccttttc cgattgcaga gcaatcgtat caaacccaag
     3901 caggaaaacg gaagtatccg ttataccagc acgtcgaact gctccttgaa ataattttgt
     3961 aaataatgtt tcactgatag aagcagcagg caatgtaagc tgaatccttg tgaaataaat
     4021 acatgcggca taaaatattg tctgaatacc atccataatt tgatgggcgt tcatcgtatg
     4081 caggggcttt tcttcccatt gtttaatagc atcctcatat ccttttcgtg ccgtttccca
     4141 gcgtgcaacg ctgttcataa gtgtcctacg taaagagcgt ggggaaaggg atttgatagc
     4201 aatcaaaagt ggctttgatt tagcagaaag ataaacatat ccattaataa ccgtatatgc
     4261 gccgtttttt ggtaataatt ttttcgcact tttaccaaac atatctatcc ataaaagctc
     4321 cgatgcacgg tttacgattt caagaccaag cgtggcgaac aaaggtgtaa caggatttgg
     4381 caagtgttct gccaaacttc cctttgtgta tacaacatcc tgttctggca atacccattc
     4441 cggtggcaga acagtaattg gacgagcttg aacaatgtac aatttatctt tttccagtgc
     4501 ccactctaca tccataggca tttgataata cttctcaatt ttctttccaa gctgtatcag
     4561 ttgcattacc tgattacgag taagagcata tttttttctc aacctttcag gaaccatggt
     4621 ttcttctgtt ccttctgaag tacgaaccgt cataatctct ttatttgcca cttcatacga
     4681 tataattcgt tcggagtcct tatccactac gattgtatcg ggtgtaacta agctagaaac
     4741 caccgcttca ccaagccccc atgcagcgtt aataatcatt tcgctacgtc taccattgat
     4801 tgggtttaac gtaaacataa taccagatga atcggaaaat gcaagctttt gtactacaac
     4861 agcaagtgcg acaatctcgt gctttatatt attcttcaca cgataagcaa tagcacgagc
     4921 tgtccataag gaagcccagc atctctttac agcgtcaata actttatctt caccttgtat
     4981 gttaagataa gtttcctgct gacctgcaaa agaagcgtca ggtaaatctt cagcagtcgc
     5041 agacgaacga acagccaccg atatgttccc taatccggca taagctgttt taattgcgtc
     5101 cgacacttcc tgtggcattt ctccattatg gaaaagctcc cctattttct tagatacatc
     5161 ttcaagttgg ttggtattat tggaatcaat accatctagc agtttattaa ttcgagactg
     5221 aatatggttc gtctcaacaa aaatctggta tgaagcagtc gtaacatgaa aaccgtctgg
     5281 cacagggatg cctgctgtca atagttttga taaagacatc ccttttccac caacaatttc
     5341 aatggttgct tgtttatgtt ccagtggcaa gacataataa atcataatca ttcacctcca
     5401 tcattcataa ttccgtgcag aaatatattc attgtttcat ccaacatttt ttccgaacta
     5461 tccgatggtc gtgacgtgta gctgattgat aacatggagt taattaacaa acgtgtcaca
     5521 agagcggtat ccgttttgcg aaagatgcct gtggtcacac cattatcaat taccgatttt
     5581 ataatatctt gatatgcata acgagcatct ttaagtctct ttcgatactc ttcttctaaa
     5641 taatcagatt ctgtatttaa ccacatcagt acagttctat actgctctgg aaatctaaga
     5701 tgattaagca tgatttttct aaggcactgt tgtggtgaag gttcaccatc aataatccta
     5761 tgaacttgtt ggatagtttt ttctatttct ttctcaactg catatacaac aatctcgtct
     5821 ttcgttttga aaaaatcgta tagacttgac tttcccatgt tggctaaaac tgcaatttct
     5881 ctcattgatg ttttttggaa tccattttca gccatgagac tcaaagcaac agcaactatt
     5941 ttttctcggc gttctgcttg ttgttcaggt gtaagcacaa ttccttttgg catagggcga
     6001 tacctccttt tagcgaccaa tggtcgtttc ttttttattt atcatacagc gaccatcggt
     6061 cacttgtcaa ggttaatttt tttcattgta tatggtactc gagataaaaa tgttcatttt
     6121 agaaattgct ctatttaagt agaaataggc ttcatcatta gaaaacaaga ctataaattt
     6181 gagatattta tcatgttcaa atttcatcaa tatcaaagtt taagatatta ataaaaaaag
     6241 aattactaag aaataacata taaagattca aaaaaggagt tgaacttaaa attcaattac
     6301 aaaaaatcaa atttaagaca actcctttat tctttatctc tactattctt tatctctatt
     6361 tatttattac aactccctaa catttaccac tactgcctta ggtctagtca tagcctcgat
     6421 tgaatactta acaccttgag ttcccatacc agatgccttt acacctaaaa atgggaagtg
     6481 gtctggacct ctttcagtct tattatttat ttgaacagta ccaacttcaa gtttatcagc
     6541 tatataaaat gccctatcaa tatcacttgt aaatactgat gattgaagcc catactctga
     6601 ttgattagca atttcaattg cttgatttat atctttaact cttataattg gaagtactgg
     6661 accaaatggt tcttcccaag caattttcat atcaacattt acattatcta acaatgttgg
     6721 atagaataaa ttattttttc taacattacc tgttattaac tttgctcctt tatgtaatgc
     6781 atcatcaacc aacccttgaa caaaatcagt tgctttatta tctataagag gagtgataac
     6841 tacttcatcc attggatttc caattgtcaa cttctctacc aatggcttta tttttccgac
     6901 taatttatcc gccacactct cttgtactaa aattctcttt actgccgtac atctttgtcc
     6961 tgaatatgaa tacgctccag atactatatt cttagctgca aaatctaagt ctgcatcttc
     7021 taatactata gccgcatctt ttccaccaag ctccaataat agtggtacca ttccagatat
     7081 tttagatata tgctgaccaa cttcagtact accagtaaag ttaataaaat taattccttt
     7141 atgcgttaca acataatccc ctatctcact acccctacct gtaatagtat taagtactcc
     7201 atctggtatc ccagcttttc tcatgatttc taccacatga agtgcgctaa tagccccttg
     7261 tgtagcaggt tttaatacaa ctgcatttcc accaataagc gctggagcaa tctttgacat
     7321 tgataaattt acaggataat taaaaggaga tatagctaaa acagtaccta atggaaccct
     7381 tgaaacataa gacatcttat ttcttgttcc accagggaaa ttatctccac taatggcttc
     7441 accctccata ttttttccca catcagctgt gtatcttaaa aaatctgctg ttctctcaac
     7501 ttctgccctt gcagatttta tgtctttggc tatttcaagt accagtatat tagctatctc
     7561 atctatattt tctaacaata aatcagcagc cttatgaaat atattggctc tcttgtatac
     7621 aggcatttca gcccatactt ttatactctc ttttgtgttt tttataacct catctacctc
     7681 atgttttgtc atagattgta ctttcccaac aagagaatta tctattggtg agtaaatttc
     7741 tattggcttt ctcgaattag attctaccca ttttccattt attaaatttt tatatacatt
     7801 ttcttttgtt ttaagttcat taaacatatt tttattcctc tctctgatat aactttgtgt
     7861 atttaaatta tagccattta ttcgttatgt aaacatttct ttttttcata aacgtttacc
     7921 tataaaaaaa attttaagtt aacgtttttc tagattcttc tattttaata acatactcgc
     7981 tccaaatcta tataggtcac actttaatat caatatacca atataccaat ataccaatat
     8041 aaatattata aacataaatc acttgacaaa tttataagta gacaatatta tatatctatg
     8101 acttaaaata tttttaaagt catagatagc atatgcagat agaaaggatt gataaaaatg
     8161 gctaaaagtt ttacagaaat agaaaaagac aatataagaa aaagactaat ttcagaatgt
     8221 gaaatcaact ggtctaaata tggatacaaa aaaactaaca ttgatgaact ttgttcaaaa
     8281 tcaggaatcg caaagggttc tttctataca ttttttgatt caaaagaaaa gttatttttt
     8341 gaagtactaa atggaatcca aaacagactt atatcatcag tagatgaaat tttaagtaac
     8401 atgcctccaa aagaaggctt tgcaaaatcc ttaaaaatgt tatatcgatt atatgataaa
     8461 aatccatttt tatattcacc aaataatcct gatttcatca gcctaataaa taaactacct
     8521 caagatatgt ttttacaact tgagtccaat aacatacaaa gtttttacga cttaattaag
     8581 agacacaatc tcaagttgaa gatagaaaaa gaaaaagcct ttggtgtttg taattctcta
     8641 ctatcaagtc tattggtaaa agaaaatatt gaatataact atattgaagt atttgatttt
     8701 atggtagata atcttgtaga acacatactt gaataacaat ttaaaataat ttttctaaat
     8761 atttctttat actaaagagg attaatatga ttgaattaat gaaaaaaata aacaatatca
     8821 tttacaaaat aaatgataaa ctaaatatac acacaaaact tatagacccc agagttaact
     8881 cattccactc tactctaagc catctataat agttcgtata tttctaataa tattagatat
     8941 taaagtaaga aaaaaattcc ttataataat tgctattata tcaattatta taaatatttt
     9001 aataatctta gataggtagg tatatatatg gtaaaatcct caaacacttt taataaaaac
     9061 ttcaacttga tgatattagg tcaaataatc tcattgtttg gtgcatcaat acttaaattt
     9121 gctttatctt tatacatact tgatataact ggtaaagcag aaatatttgc tactatacta
     9181 gcagtatctt caatcccaat tatagtattc tcccctattg gtggagctat tgcggatata
     9241 ttcaacagaa gaaacttaat ggttattttt gatttttcaa gcagtcttac agtactaatc
     9301 ttagcagtat tcttattcaa caataatggt tcaatattac ttgtaggaat tattatgacc
     9361 atattatcag ttataagcac aatgtatcaa ccaactgttc agtcaagtac acccctttta
     9421 gttgataatg agcatttaat gaatgcaaat ggtattgtag ctggagttgc atctcttaca
     9481 aatattgcag gacctgtatt aggaggtgtc ttgtatggtg tgattggaat taatgcaatt
     9541 atagttataa gttgtgtaag tttctttcta tctgcaatta tggagatatt tatacagatt
     9601 ccttttatca aacaagagca aaacagcaac attataaaaa ctatatttac tgatataaag
     9661 gatggtattg ttcatatatg taaagaaaat agatttatac taaaaatact ttttttagct
     9721 atagcaataa acttattttt aagctctatg ttaatagttg gtatccccta tatagtaaaa
     9781 atcactatgg gagctagtaa cttcttatat ggtctagcag aaggagcaat ttcttttagt
     9841 tcactggtag ctgctgttag tattggtatt ttctcaaaac atctaaaaat cactaatttg
     9901 cacatttcct ttattatatg tgcagttatg ttcataccaa tggcggtttc agtatatcca
     9961 tttatattaa acacaggatt tccagttcca tttttaatct ttatagtatc atctatgcta
    10021 atatttttta caacgactct tatctcaata tatgttataa cagtcacaca attaaaaact
    10081 ccaaatgaac tcttaggaaa agtaatgtca attctttttg caggaacagc aattgcaatg
    10141 cctcttggtc aggttatgta tggtaaactt tttgatttat tatcagatag agtgtacatc
    10201 attatactag gagttggatt tattacacta cttatagcat ttgtatcaaa gataacattg
    10261 aggaacgaaa atctttagat acaaagaatt tagatataaa gatttaattt tcactaattt
    10321 ctgaatttaa atgcaaaatt gcgtatcagt atgttgacgt tttagaaatt gattgatata
    10381 atcaatggaa aataaacttt ataaaaattc aaaatattca gctagaggag tgataaaaat
    10441 gagttacatg ccaactttag aacaagcatg ggaaatatta agaaaataca ataaggaaga
    10501 atttcatata agacatgctc aaattgtatc tggagttatg agatactatg caaaggagta
    10561 cgaccctgag agagtagact tttgggaaat agttggactt ttacatgatt tagattttga
    10621 aatgtatcct gatgaacatt gtgttaaaca aagagaatta atgacagagc ttgatttaga
    10681 taaatcaata atcgattcta caataagtca tggatatgga ttaactggtt cagatgttaa
    10741 accagagcaa tttatggaga aagtattatt tgctgtggat gaacttactg gtttaattgg
    10801 tgctgttgca ataatgcgtc cttcaaagag cgtttctgat ttagaattaa aatctgttaa
    10861 gaagaaattt aaagataaat catttgctgc tggctgttct cgagacgtta taagaagtgg
    10921 tgcagaaatg cttggatggg aacttgataa gcttatccaa acaactatag aagctatgag
    10981 aagcttgatt ccagagatgg caatatagct cctcatacat ataccataga agttacatct
    11041 ttaaagagca tttctctttt tgatgcaact tctttttata ttttgtagaa taaaaagttt
    11101 aatattttaa actcactata taatctataa taaatactat aaataatata atgtataata
    11161 gtcataaact ttctaacctt aaaagtttct aatactcaat aaacaattct aatatacaag
    11221 tggcattata tgatttaatt atatatcttc aatttatata tta
//
LOCUS       DAMKPG010000106        11204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMKPG010000106 DAMKPG010000000
VERSION     DAMKPG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.111"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            112..657
                     /locus_tag="PC621_003081"
     CDS             112..657
                     /locus_tag="PC621_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF4110220.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            670..1683
                     /locus_tag="PC621_003082"
     CDS             670..1683
                     /locus_tag="PC621_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF4110221.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1905..3878
                     /locus_tag="PC621_003083"
     CDS             1905..3878
                     /locus_tag="PC621_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF4110222.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3947..4306
                     /locus_tag="PC621_003084"
     CDS             3947..4306
                     /locus_tag="PC621_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110223.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4341..4697
                     /locus_tag="PC621_003085"
     CDS             4341..4697
                     /locus_tag="PC621_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF4110224.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4734..6026
                     /locus_tag="PC621_003086"
     CDS             4734..6026
                     /locus_tag="PC621_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF4110225.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            6462..7100
                     /locus_tag="PC621_003087"
     CDS             6462..7100
                     /locus_tag="PC621_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110226.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            7107..7949
                     /locus_tag="PC621_003088"
     CDS             7107..7949
                     /locus_tag="PC621_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF4110227.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            8010..10637
                     /locus_tag="PC621_003089"
     CDS             8010..10637
                     /locus_tag="PC621_003089"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF4110228.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            10851..11096
                     /locus_tag="PC621_003090"
     CDS             10851..11096
                     /locus_tag="PC621_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF4110229.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT         4461 a         1144 c         2162 g         3437 t
ORIGIN      
        1 aaaaacattt acaatattag catatagtac agaaaaaaga tgtaatttgt aaaatttatg
       61 ttaagcagta aaattaaaaa tttaaagata ttatagggag gttataattt tatggaaaac
      121 gttattacag gtctaagtaa aggtgcagag tggtttatcg gtttgtttca gaaaggggga
      181 gaacaattta taggtcttgt ttcaggtact cttccaacgc taattgtatt aatggtagct
      241 attaactctt tgattaagat aattggtgag gaaagagtta ataattgggc ttctaagcta
      301 ggaaagaact cttttacaag atatattcta cttccattag tatcagtatt tttcttggga
      361 aatcctatgt gctatacatt tggtagattt ttaaaagaag agcacaaagc aggattttat
      421 gatgcagcag tttcatttgt acatccaatt acaggtcttt ttccacatgc aaatgcagga
      481 gaactttttg tatggttggg aatatcagca ggtttgacta ctttaggtaa agaaactact
      541 acactggcat tgtggtattt tatagttggt ctaattgtaa tttttataag aggtatagta
      601 acagaaaaaa tgtatgcttt tctaacaaga aaaaatagag caacaaataa ggcttaggag
      661 gtagataaaa tggaggagaa taaaatatta aaaatagaaa agggaacttc aggctgggga
      721 ggtcctttgt atataaaaaa agaagggaat agaaataaga tactatcaat gactgctggt
      781 ggtatccatg aagtaacatt aaaaataaaa gaactactgg gttgtgaaat tgtagatgga
      841 tttaaaactg gagtttctga tgaagaagta gcagtagtta ttattgattg tggtggaact
      901 gctagatgtg gagtttatcc aaagaaaaaa atacctacaa taaatgtaaa tccagttgga
      961 aaaacaggtc ctttagcaaa atttataaca gaagaatatt atgtatcaga tgttaatcct
     1021 aactgtataa gtgttgtaga tggggaagat atgccacaaa aatctcaaga aaataaaagc
     1081 gaaaataaga gttctattag aaagcctgat aattatgatg aggttaagtc aaaagcacaa
     1141 ggtgaatatg ctaaaaagaa cataatactt tctattggtc aaggggcagg gcaagtagta
     1201 tcaaaatttt atgatgcagg tcgagacact atccaaatgg taatgaataa cgtaatacca
     1261 tttatggcat ttgtaagtat gcttatggga attattttag catctggact gggagactgg
     1321 attgcaagag ttatatctcc acttgcaggt aatattggtg gattattgat tatatcagta
     1381 atttgcacac ttccattctt atcaccaata ctaggacctg gtgcagttat agctcaagta
     1441 gtagggactc ttgtaggtac acaaattggt cttggtgcaa taccagcata tttagcactt
     1501 ccagctttat ttgcaataaa tgggcaagct ggatgtgatt ttgtaccagt aggattaagt
     1561 ttgggagaag cagaaccaga aactgtagag tatggtgttc cagctttatt ttactctcgt
     1621 ttaataactg gtccaatagc tgttattatt gcatatggag ttgcagtatt tgcattgaga
     1681 taatatttaa tgtcaatata aggcttggaa ttaattaata aaattgtaaa tataaattta
     1741 agctagtcaa gattttttgt taatgaaaga ctagctttta tactattaat tagctgtaat
     1801 tatcgagtta gattaatact aaattttcct ttattaaagt gaaattttat gtattaattt
     1861 ggtttcaaat ttgtactaga atgaaatcaa ggaggagata caaaatgagt gtaactgaga
     1921 aaattccttc tattgaccgc aagatattta agatattgat tatgtgtagt aaaaaagagt
     1981 ttgtgagtat taattcaata gcaaatgaat tgaatgtcac aactagaagt gtaaggacgt
     2041 atataaaaca attgaataaa gatttaggta atgatatagc agtaatcaaa tatataaagg
     2101 gtcaaggcta taaactagaa ataaaagatg agcagatact caataagatt attgatatga
     2161 atagaaaaaa tgtattttca ttaaattcaa aagaagatag agtagagttt atactaaatt
     2221 acttgataga actagatgga tttattacac ttgatagttt agctgatgag atgtgtgtag
     2281 gaagaactac tttagtaaat gatttccagt atgtagaaaa agttttggct tcatataatt
     2341 taaacttgat taaaaaacag aatacaggta tgaaattaaa tggaaatgaa cttgatatta
     2401 gactgtttat attaaatcag ttgtataaaa actcaaggaa agatttcagt aattcaaagt
     2461 attttaaagg aatcaaaaaa gaagaaatca taaatttaga agaaaaacta ttgactttat
     2521 ttaaagaaaa taatttttat gttacagatg aaatgttaag agaagttatt aactacatag
     2581 tagttttagt ctatagagta aaagaagaaa agaaagtaaa agactatgat gtaaagtttg
     2641 atttacttaa atcatatgat gaatacttta ttgctaggga aataaaacat ataatatcag
     2701 gaatgtttga atgtacttta aatgatgaag aaattattta tttaactata ccattagtta
     2761 gtggaaatgc accagcatca gaatgtgctt taaatagtag tagaattagt aaaaacattg
     2821 atgagttgat ggaaaatata tttaatcaaa tatatgtcga tatgggtatt tttataaatg
     2881 aggatgaatt aagagtaggt ctaggatatc atcttagttt tactttaaac aggctattat
     2941 ttaatataaa gttaaaaaat gttttgttag aagagattaa gcaaaattat atacttccat
     3001 ttaaattagc acaaatagca ggaaaagtaa tagagaaaaa gtataactta gaagtttcag
     3061 aagatgagat tggatatata gcaatccatt tcagtggata tttagaaaga aatagcagta
     3121 gattctattc aataaaaaaa atagcaatta tatgtagtac aggtctagga acagcaaaac
     3181 tattgaaaat aagagtagaa aaactgatag gaaataatcc taaaattgat actctttcaa
     3241 gttttaattt gagaaatatt aacttagatg aatacgatat agtttttaca actattgatt
     3301 tagacacatc gaatataaat acaatagtat taaaaataaa tacaatattt gatgaaaaca
     3361 aattaagaga acaattaaaa acagtattgt gcctaagaga agggaatatt gacactacta
     3421 attctacaaa tttactaata aataatttgt tagatgaaga taaatttatg atattaaatg
     3481 aaaagactat acttgcttca ttagaaaaaa tgatggacca tcttatggat ttgggatgta
     3541 ttgatgataa gtttagaaaa aatatagtca atagagaaga aaaatctccc acagtatttg
     3601 ataaaggttt attatttcct cactctgtaa atgaaaaatc agataagttt ttaatggcag
     3661 ttggaatttt agaagaacca atagaatatg caaatagaag tataaaaata attcttatga
     3721 caatgtttcc atgtgagaat aagatagact cagacttatt agtaaaaatt tatgaagaag
     3781 ttttaaagat aggccaagat gtaaaattaa cgaataagat aagtaaatgt agaacttttt
     3841 tagaatttaa aaaggtgtta cttaaaaacc ttatataact attgtacagc atttatagtt
     3901 gttatacaga tttaaaaata atgtgatttt tggaaggtgt ataaatatga aattattagt
     3961 aattttattt gttgcttata ttttaaatac tatatttata atgaagcaga ataaattgta
     4021 ttttaaaact ttaaatagag ctaaaaaaaa gggtgatata gtgtctacag gcaaaaagaa
     4081 aagctatttt tctaaaggaa gtatagccat tatatcttca gattcagatg ggtttataaa
     4141 gtttggagaa attttaaaag ggcgtacagt aatggctaaa tttaaagaga ttgaagaaat
     4201 agaaggatta gatatatata gtgcagagca aaaatttaaa gatgaagaga gtattgtaca
     4261 agctataaaa tttataaaag aaaaaataaa tgtttctttt aattaactca atataaatat
     4321 aaattatcag gaggaatgtc atgaaatatg aagttaagat aagtgggata ggcagtttag
     4381 ttaaagagtt aatggatgaa agcaattgtc ttattatata tgatgaaacc attaatgatg
     4441 acgacttaaa ggatatatct gtaattcatt cgatagcaac attaaaaagt gatgttgaaa
     4501 taggagatac acttactata gggaatagag attattgtat agtttcagtt ggtgatattg
     4561 ctcagaagac tcttagagaa attgggcatt gtacaatcaa atttgatgga aaatgtgaag
     4621 taaatttacc aggagaaata catgtggaaa taggaaatcc agatataact attggagatt
     4681 taataactat ttcttaattt gaaaataaaa ttattataac aggaggaatt ggtatgttag
     4741 atttaaacag aaaacttgca aaattggaag aggaaggtaa aaaaataaaa gtagctctta
     4801 taggagctgg gcatatggga aatggaatgg taagccaaat ggcaaatatg aaaggtattg
     4861 aagcttctat tgttgtagat ataaatttgg agttggctca taaagctttt actgatgcag
     4921 gaatagatga agaaattata gataatgtaa caaatgaaaa ggatgcagaa ataaaattac
     4981 aagaaggaaa agtattgaca tgtaatgact ttcttgtggc ttgtaaaact aagtcaatag
     5041 atgtagttat agatgcaact gggggtattg caattggtgc agagatagca ttaaactcta
     5101 ttttaaataa aaaacatata gttatgttaa atgctgaaac tgattgtgtt gttggaccaa
     5161 tacttaaaaa attagcagat gatgctggag tgatatttac aggttctgca ggagatgagc
     5221 caggtgcagt aatggaatta tttgattttg cagatgcaat ggggtttgaa gttagagtga
     5281 taggtaaagg taaaaataat aaattagatt tagattgtaa tcctgataca gttagagaag
     5341 aagcagaaag aaaaggagct tcacctcata tgatagcttc ttttaaagaa ggaactaaaa
     5401 caatggtaga aatggcttta atgtgtaatg caactggatt tgtgcctgat gtaagaggtg
     5461 gtcatggtat tgaagctaca gttaatgaag tacctaagaa atatgcatta aaaagcgaag
     5521 gtggagtatt agataattat ggtgtagttg attttgtaaa tggtatagca cctggagttt
     5581 tcgtagtagt agcccataaa ttaaaagcag ttaatgatga actaaaatat ttaagtatgg
     5641 gtgatggacc taattacata ttatatagac catatcattt atgtagttta gagactcctt
     5701 tatcagcagc tatggcagtc ttagaccata aggctacaat agtacctaaa gctggactag
     5761 tagcagaagt tatgactata gctaagaaag atttgaaaaa aggtgagttt atggatggtt
     5821 atggagcata tacatgctat ggtactatag aaaaatacga tgtagcaaag gctatgaatg
     5881 ctgtacctat tggtttgata agtaaaaaga ctaaagttgt aaaagatatt aaaaaaggtg
     5941 aagttataac ttacgatatg gtagagatag aaaaagatac tactttatat catttaagac
     6001 aattgcaaga aaaaatattt gggtaagatt tgatggtaat ataaatagat attagtaaaa
     6061 agggttgatg gttattttta aatcaaccct tttaaatttt tatatattct tgttaggata
     6121 ctttctttaa aagtatatta taggttattt gtagtcgtaa aaattatttt gaattagttc
     6181 tgcaatttta tattcacatt gaaacaatat tattaaaaat tttaatataa aatgaattaa
     6241 aatgaccaat tatgtattat aatattaact gtaatactaa tatagttcta ttaattaaag
     6301 aaatatcccc cattaatata taataaatat ctttaagcga gagaacttag taaaaaatat
     6361 atcgtattca ataaaaaatc tgtttacaga atggtatata tagttatata atatactata
     6421 taagatgcga aaagataaaa agtatagcac aatgaaagaa ggtgattatt attcagctta
     6481 atgaaagaca actaaaaatt atagatatag ttaaggaaaa tgaaccaata acgagtgaaa
     6541 gtattgcctc cagcttgaat gtgactcgtg ctacacttag atctgattta gctatattaa
     6601 caatgacagg aatattagac gcaagaccaa aagttggata tttttattca ggagttagtg
     6661 aaataaattt aattggtaaa agtgtaaaag aaaaaacagt agaggatata atgagcatgc
     6721 ctgtactagc taagaaggat gaaagtatat atgatgtaat agtcactatg tttttatctg
     6781 atgtagggag tatagttatt atagatgaga atgaagagtt atgtggagtt gtttccagaa
     6841 aagacttgct aaaagcaact ataggaggtt ctgatataaa taaaatgcca ataggtatga
     6901 taatgacaag aactccaaat gtagtcactt taactaaagg agcaagtgtt ttattagctt
     6961 caagaaaaat aatagaacat gaagtagatt cgattccgat tgttgaatat aagggagaag
     7021 acaaaaatca tatgagagtt gttgggagaa tatcaaaaac caacataact aagctattct
     7081 tagaaatagt tgacaattaa ggaggtatgc tggtgaaaaa cctaattata tatgcagtat
     7141 cagattcagt aggggagact gcacaacaag ttgcaaaggc atgtatgtct cagttttatg
     7201 tgaatgaaac ttatgaaata aaaagatttc catacatgat taataaagga gtactacttg
     7261 aaactttgga gaatgcaaaa gctgaaaacg ctttaattgt atatacatta gttgatgaag
     7321 aattatgcag tattgttgaa agatattgcg aaagagaagg tcttagttgt atagatttaa
     7381 tgacagatat actaagagaa ataagcaaga gaacaggaag aaaacctaaa agagaagctg
     7441 gtataattag aaaattagat gaatcttact ttaagagagt tgaagctata gaatttgcag
     7501 tgaaatatga tgatggaaaa gaccctagag gtgttcttca agcagacata attttagtgg
     7561 gaatatcaag aacatctaaa actcctttga gtatgtattt agctaataaa aatataaaag
     7621 tagctaatgt tccattagtt ccagaaatac caattcctaa agaagtgttt gagatagata
     7681 ctaaaaagat aataggtctt actaattcac ctgaaaagct gaatgagata agaacccaaa
     7741 gattaaaagc tttaggattg tccagtaaag caaactacgc taatttagaa agaatacttc
     7801 aggagttaga ttattcagaa gagataatga aaagaattgg atgcccagta ataaacgttt
     7861 caaataaggc aatagaagaa acagcaggta taattttaga cataatgaaa gaaaatggtc
     7921 taaagatata caaagaaata gaaatttaat aatgtctatt aataaaaatg atataaatta
     7981 aaataaaaag attaatgggg gatttaacaa tggaaactaa gtatgtttat agttttggtg
     8041 aaggaagcaa agacatgaaa tctttactag gaggtaaagg tgccaattta gcagaaatga
     8101 ctaagatagg cttacctgtc cctcctggat tcactataac aacagaagca tgtaatgatt
     8161 attatgttaa caatgaaagt ataagagcag agataataaa agaaatagaa gagcatttag
     8221 ctactttaga aaaagactta aataaaacat taggatgcaa taagaatcca ttattagtat
     8281 cagttcgttc tggtgcagta ttctctatgc caggaatgat ggatacaatt cttaacctag
     8341 gtcttaatga caatagtgtt gttggcttag cagaggctac acaaaatgaa agatttgcat
     8401 atgatagtta cagaagattc atacaaatgt tttctgatgt tgctatggaa gttccaaaat
     8461 ataaatttga aaatgtatta gacagagtaa aagaagcaaa gggatacaca gttgatacag
     8521 aacttacaac tgatgattta aaagaaatag taaaagaatt taaagcaatt tataaaaaag
     8581 aaataaaaaa tgatttccca caagacccaa aagagcaatt aatgcttgca atagaggctg
     8641 tatttagatc atggaataat cctcgtgcaa tcgtataccg taaattgaat gatatagctc
     8701 ataacttagg aacagcagta aatattcaat caatggtatt tggtaatatg ggagaaacta
     8761 gtggtacagg tgttgcattt actagaaatc cagctacagg agaaaataaa ttatttggtg
     8821 aatttttaat gaatgcacaa ggtgaagacg ttgttgcagg tataagaact cctcaaaata
     8881 tatcaactct tgcagatgta atgccagctg tattcgatga atttgtaaaa attactcata
     8941 tacttgaagg tcattataaa gatatgcaag atatagagtt tactatagaa aatgaaagat
     9001 tatatatact acaaactaga aatggtaaga gaacagcagc agcagcaata aatgtagcgg
     9061 ttgatttagt tgaagctgga ataattgatg aaaaagaagc tatcatgaga attgagccta
     9121 atcaattaga ccaattatta catccaaaat ttgaagataa ggcattaaaa gaagccaagg
     9181 ttatagcaaa aggtttacca gcatcaccag gtgcagcaag tggtaaagtt tatttcaatg
     9241 ctgatgatgt agtaaaagca aatgaaaaag gagaaaaagt agttttagtt agattagaga
     9301 catctccaga agatatagaa ggaatggtta aagcagaagg aatccttaca gctagaggtg
     9361 gaatgacatc acatgcagca gttgttgcta gaggtatggg taaatgttgt gttgctggat
     9421 gtggagaaat aaaagttgat gagtttaata aagaaataag agcacttgat gatgtagtta
     9481 taaaagaagg tgaatacatt tctatagatg gttctacagg aaatgtgtac ctaggagatg
     9541 ttaagaaaac agaagtatct ttaactggaa actttgaaaa attaatgaac tgggtagaca
     9601 aacacaaatg tatgatggtt agaactaatg cagataatcc tagagatgct cgtgcagcta
     9661 tagagtttgg tgcagaaggt ataggtctat gtagaactga gcatatgttc tttgatgaag
     9721 ctagattgcc agcagttaga gaaatgatat tatctaatac agtagaacaa agagaaaaag
     9781 ctcttgaaaa aatattacca atgcaaagag aagattttgt agagttattt aaagttatgg
     9841 atggaaaacc agttaatata agattactag acccaccact acatgaattt ttgcctcatg
     9901 atgatgagac tatagaagaa ctttcaaaat ctatgggaat aaaagtaagt gatattaaga
     9961 agagaatagt tgatttagat gagtttaacc caatgcttgg acacagaggt tgtagacttg
    10021 caataactta tcctgaaata tgtgtaatgc aatctaaagc tataatacaa ggtgctatag
    10081 aagcaatcaa agcaggtgtt aaagtttctc cagagataat ggttccatta gttggagaag
    10141 taaatgagtt aaaaataata agaaaaatga tagtagaaac tgttgacgct ataataaaag
    10201 aagaaggtat agaagttcct tatactgtag gtactatgat agaaatacct agagcttgtt
    10261 taactgctga tgaaatagca caagaagcag acttcttcag ttttggtact aatgacttaa
    10321 ctcaaatggc atttggatat tcaagagatg atgctggtaa attcttaggt caatatgtag
    10381 atgaagaaat attagaaaaa gacccattcc aagtactaga ccaaaatggt gttggtaagt
    10441 tagttaagat gggagctaaa ttaggtagag aagttaaacc agaattaaaa ctaggaatat
    10501 gtggagagca tggaggagaa ccatcttcag tagagttctg ttatagtgta ggacttaact
    10561 atgtatcttg ttcaccattt agagtgccaa ttgctagact tgcagcagct caagcatcta
    10621 taaaaaatcc tagataataa tagactgcaa aatatgtaac taaataaaaa taaaatcccc
    10681 tattgttcaa tgtatcaaat taagtatttg taaattgaat ataggggatt tttattataa
    10741 aaatttaact tatacagttg taagtgaaac gtatttaatt atattgcata tattattgta
    10801 gagagaaatt tcaactatca attaaattga gtttactgga ggggataaag ttgacaaagt
    10861 gtcctaaatg tggaaaggaa gtatcatcac gtccaggtag tatatgccca cgatgtggat
    10921 ttaaagtagc agatgagagt ctaaaaataa gatgtccaga acctagttgt agggctttgg
    10981 tatctaggaa actggattat tgtcctaaat gtggatgcaa acttagagga tataatattt
    11041 ctgaatttgt taacatggta agatgtaaaa tagatgagac atttgataaa aaataattaa
    11101 atttagagtt aagcttttga gggtgtctca aaatgaactt tttagttcag aaggcactct
    11161 ttttgtatgg aatcaaatat atttttataa tttaaacaat aaaa
//
LOCUS       DAMKPG010000107        11192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMKPG010000107 DAMKPG010000000
VERSION     DAMKPG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.112"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            338..949
                     /locus_tag="PC621_003091"
     CDS             338..949
                     /locus_tag="PC621_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110230.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            977..1159
                     /locus_tag="PC621_003092"
     CDS             977..1159
                     /locus_tag="PC621_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110231.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1399..1941
                     /locus_tag="PC621_003093"
     CDS             1399..1941
                     /locus_tag="PC621_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF4110232.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1973..2635
                     /locus_tag="PC621_003094"
     CDS             1973..2635
                     /locus_tag="PC621_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HDF4110233.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2825..3142
                     /locus_tag="PC621_003095"
     CDS             2825..3142
                     /locus_tag="PC621_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110234.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3164..3607
                     /locus_tag="PC621_003096"
     CDS             3164..3607
                     /locus_tag="PC621_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110235.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3825..4268
                     /locus_tag="PC621_003097"
     CDS             3825..4268
                     /locus_tag="PC621_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110236.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4399..4819)
                     /locus_tag="PC621_003098"
                     /pseudo
     CDS             complement(4399..4819)
                     /locus_tag="PC621_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4797..6074)
                     /locus_tag="PC621_003099"
                     /pseudo
     CDS             complement(4797..6074)
                     /locus_tag="PC621_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6097..6639)
                     /locus_tag="PC621_003100"
     CDS             complement(6097..6639)
                     /locus_tag="PC621_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110237.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6679..6897)
                     /locus_tag="PC621_003101"
     CDS             complement(6679..6897)
                     /locus_tag="PC621_003101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110238.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7093..7836)
                     /locus_tag="PC621_003102"
     CDS             complement(7093..7836)
                     /locus_tag="PC621_003102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110239.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8136..8693)
                     /locus_tag="PC621_003103"
     CDS             complement(8136..8693)
                     /locus_tag="PC621_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4110240.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8888..9076)
                     /locus_tag="PC621_003104"
     CDS             complement(8888..9076)
                     /locus_tag="PC621_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110241.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9087..9662)
                     /locus_tag="PC621_003105"
     CDS             complement(9087..9662)
                     /locus_tag="PC621_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110242.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(9995..11134)
                     /locus_tag="PC621_003106"
     CDS             complement(9995..11134)
                     /locus_tag="PC621_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF4110243.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
BASE COUNT         3949 a         1525 c         1360 g         4358 t
ORIGIN      
        1 taaattttta ttcaaagaat acacataaat atttccagac tcacttattg tataagttat
       61 attctatttt gtatgaagtc aattaaaaac tgaactatat taaaattaat tttataaata
      121 acaaatataa ataattacaa aaccttaact gcgttaagtt tgaaatataa taatattaaa
      181 aacacttctc tcaaatcaag tatcttttgt tatattttca taagttttaa aaatataaat
      241 tttttaaaac ttgtgtccaa aaaacaaaat taaatatgtt atatatgtaa agggttaatt
      301 ttaaaacctt aaatttatta tagttagggg gatttaaatg aatatcaaaa gaattaatct
      361 ccttttatca acagtgatgg cagctgtgat aatatcttta ccacttgctg ttacagcaaa
      421 tgcaatggaa aaaacagaat cagataataa tgtgaaaata gagttgtctg acaaagaagt
      481 agaggaatta cataattact ggcctgagga taaaattttt tcagatgaaa agattgatga
      541 gatcttaatt aaaacaggtc aatttactca aaaggaattg gataaaatca atcaagaagt
      601 ggacgattct gctaagaaag taaatcaaaa ttctcgtgtg aaatatataa atgggtataa
      661 caaatacaga acttttacta aaaatggtaa aaactattta tatatttatg taagtggaaa
      721 gacattaaag aaaataaaag ctggaacaga tcttgcatct acttttggag gttttttacc
      781 aaaaatttgg gtagagcttg atgtagtggc aattggaaag ataataacaa ttaacatgga
      841 tggtataaat actaattatg ggattgtttt atcatatata gaggataagt ggaattttca
      901 gggtgcaaca tatacttata gatatgatca ttggttctat caaacttaaa agacatgctt
      961 ttttaggagg aataatatga aaaaaaggct ttataaggtt tttttaaaaa caattccaaa
     1021 cctgataatt gtgatattat cacttgcatt atcattttat actaatacat ttgatattga
     1081 tttaaaaata ttgcttttaa tacctttagc atatgtactt ttgtttatat actattacat
     1141 caaaacaaaa aaagattaat ttttatggaa ttagatatat acgaaaataa ttaatttaaa
     1201 aatttaactt tgagaatcat ttgtagagtt aaggccctta taatcccgaa aatggatttg
     1261 aagggccttt ttactagata tctttaaaaa gcttgttaag catagttata ttctatttaa
     1321 gtagagttaa ttatatattt atgttataat tgaactttaa aatattaatt cagaattttt
     1381 atattggagg aattctaaat gttaatttat ctagctatac ttgaactcga agaagataaa
     1441 ataaagtttg aaaaggtata tgaaaagtat agacaaataa tgttttatgt agccaataaa
     1501 atattaaagg atgatcattt atcagaagat gcagtacata atgctttttt aagaataatt
     1561 aaaaatatag ataaaattga tgaggtagac agtccaagaa cgaaggcttt tatcgttata
     1621 atagtagaaa ggattgctat agacttttac agaaagagaa aaagagaaaa ggtatctgat
     1681 attgaagaag agtataaaaa cagggagata aatttttcta tagaagataa agtatgtgaa
     1741 agtaatttag ctatagcact tgcaaaactt aatgaaagtt actttcaagt gctttcctta
     1801 aagttccagt atggtttttc aaataaagaa atagcaaatg cattaaattt aggggaggaa
     1861 aatgtatata agagaattca gcgtgctaga aaaaaattaa aagaaatatt agaagaaatg
     1921 gaggttggaa atattgagta ataaaaaaga tgattttgat attacggatg atatgcttag
     1981 agaaagtatc ccaatagcga caaaaattat tattgatgac cttctttcag atgatgaatt
     2041 aaatcatgtt ttttcaaaag gttttgagcg aaaaatgaaa aaactcatta agcaacaaaa
     2101 aagaagtagt cttacaaata aaatcatttt ttattcaaaa caaacagcta ttgtattttt
     2161 aatagtttta gctagtttac ttactattac tatgagtagt gaggcattgc gttcacgttt
     2221 ctttagatta gtaataagag tatatgaaga cttaacttca tttgtttttt caactaatgg
     2281 agaagataat cttagtttaa aaaataaaga acctgagtat ataccaaaag gatttaaaga
     2341 agttgataga gttgatgatt ttgttattac atatagaaat gatgaaaata ttgagataca
     2401 atatgtaaca gataaaatta gttcaaattc tattatatta gatacagaaa atgcaaaagt
     2461 taaagatata tttgtaaatg ggcataaagc aaaatatata gtaaaaggaa ctttcttaca
     2521 gttattttgg aatgacaaca actttatata tttattaaat gtagattatc aaaatagtgg
     2581 agaatctgaa ttttatgaag atattttgat aaaaatagcg aaaagtataa aataattttt
     2641 ttaagtgtcc aagggtagcc tcttcattcg ttatacaagt atatatgaaa atgtgaggtg
     2701 atttttatgg ttttaaaatc tacatttacc tgagcgttaa aaatattgtt ataggaaaaa
     2761 ttaatacatt atagttataa catgcttgca ataggaataa aactttaaaa ttgaggtgta
     2821 aaaaatgaaa tcagatttat atttatgtaa aagtaataaa ttgtatagag acaacgaagt
     2881 tctagaaaaa tttatggaag atttaagttt tcttatagac gattataaat tctcgttaga
     2941 aacaatatct attctatgca aaataaatat tgagaaatta actaatttct atgcaggaaa
     3001 aggatatata ctgtatgaag aaatgtctct tattgaaagt gtacttgcac cttttttaag
     3061 tgcaattgat attgctaaac ataattatga tgttgtcatt aaatcgaggg aaagcgataa
     3121 acctttgata ttcgaaaatt aatttaaaac tgggggaata cacatgaaaa gattatttgc
     3181 gttattactt actttaagtt tagttatatt accatcaact ttagtatttg ctaatgaaaa
     3241 agctgaaaat gattcaattt atgctgaggc gccattagtg tttcaaagta atgaaaaagc
     3301 atatacacat ggtagtggag gaactgcata tataggttat atgtcaagta aacatcttaa
     3361 ttggaggata acatcttcaa aaggaatagt tatactattt tcaggaactg ttgagattag
     3421 aaattcaaaa aatatgcttg tagcatcata tccaataagt ggtggaggat cttcaactat
     3481 atctggacaa atagatgtta aatatcttaa aaaaggggat tataaagcta tattgaaagg
     3541 tacaagtaca acaggaggcg gagtcttagt agtaaaatct ggtctagttc agatgtttac
     3601 aatttaatat tataagtaaa ataaaaatca tgaatttatt gggggatagt cacaatacat
     3661 attatttaca gatattcata actattatag ttaaagacat tctattataa agaaaatgta
     3721 taatttaaaa ttataataaa aaaaattatc attagtgtcc aaaaatcact tcttcattcg
     3781 ttatataagt tatataaata gaaaacaaat gaagaggtga ttttatgaat cttagaaaaa
     3841 cagttataag tggtatgtgt gctttagtat gttcatcagc agttgcaata cctgcatttg
     3901 ctgatgaata tcaagataag aatattgaac aatattcagc agtaaactat atagatgata
     3961 ctatggtgaa aatgtcttat attaagtctg gtacttgcaa tttaagtatt tcaggaagta
     4021 cagctaaagt aacatgcttt gttagaggag catcaaatgt tacaagcaca tcaattattg
     4081 ctagattaca gaaaagcaat gataatggga aaacatggtc aacaatccaa acatggaatg
     4141 cttcaggaaa tatttcatgt tctctttcta agtcaaaaac tataggaaaa ggatattaca
     4201 gagttcattc cacaataaaa gctaactctg aaagtaaaaa tataataagt agtacaaaga
     4261 aatattaaaa atagatattg tatttatgag ttgtagtaaa caagaataat agttttttaa
     4321 aagcaaatat aattaatttt aaatagagtg ccaatataaa ataggtgtat aatatttata
     4381 cttttttata ttggcactct attttataaa ataagttatt atagaaaatg aaacataatt
     4441 tatatataaa ttttctgttt gacctggtat ataatctttt atatcacata tcagtatttt
     4501 cccatcatta taaccccaac caatcacagt tgcaggtatc atatcagctt ctccatcata
     4561 aatgattgta tgtttataca tgatttttcc ctcgctatta tctatagttt tattcaaaat
     4621 accttctgct attaatttag caactatgtc tttaatagtc tgtatctgct ttactgtcta
     4681 caaaacatac ttcaattaat atcgctggag cttttgtatg actaagccaa tacaattccc
     4741 ttacgtccga ttttgcacct ctattttcag cagatttata tatatcttca gtcaatttag
     4801 ttataatctt ttgattcatc taataaaatc tctccctctg aaactatttc ctgttctttt
     4861 ttagcctttt ccatttctgc atgaggatta tcaataaaac ttggttgaga taatccggtt
     4921 tcagtagata atttatctcc aagttgactt aatatatttg ctacaactaa atcattatgt
     4981 ggtatcttag gggtaaattt agccttaata tctttataat catagtcaaa accatatttt
     5041 ttattaagcc aaatgcacaa taatttgtac ctattaaata aagtatctgc aatactatca
     5101 ttattctggc tacaaatcca ttctgtactg attaatcttg ctacaactgc taaacttgat
     5161 gtattacttt gcattttttc attgtgattt atatgagatg taattttata tatgttttct
     5221 tttattgaag atagtgtatt ttgaacaaat gtatcattca tgtctttaat aagccattct
     5281 atttttccat ctccattaac ttgaagtatt ccaagttctt tcatccttgg caaatcatct
     5341 tctttaatat tacattcaga gaaggttaaa taagcatttc taaaatcact aatttcatta
     5401 cttatatcac ttaaattagt ttcataagca tcttgtaatc cttttatatc attgaatatt
     5461 gtattttttc ctagttcttc acttctttta caaattgata ttggaacatt accattaaat
     5521 atattattta ctgtaggttt ttctatctct ttaaaatttg aatcaaaatg atatatgtat
     5581 tctttatcat atacatctat ataaatatca tctttaaaat cttttttaaa ttctctaaaa
     5641 aaatagatgg tctctccatc ttcattttca taatggcagc catctgttgg ttttattatt
     5701 cttactttca tttcattgtt ttttacatag taaagttcat agcaaaatcc aaataatagt
     5761 gtatatctca ataaatctga atcgtgcttt ttactccaat ggcaagtata gtattctaaa
     5821 tcatttataa tcttctcatc acctaatcta cttgtatagg tgattttatt tgcaagagaa
     5881 taagcgacct cttcattaat aaactttttt aagaaattag tgtttatctt attattagac
     5941 ctcttggtaa ccattttata gttactaata gcatctgttt cacctttata atacttatac
     6001 atagtgttgt attcttgctt ttttaaactc tagtcttcat atatttctct taataaactt
     6061 aaatctattt ccatttaatg catcacctcc atacaatcaa agacctaaat ttcttctatc
     6121 taatagtgta atactttcat ttactttaat attaaaaatc ctatttataa attctgctga
     6181 tatatctggt gcatcatcat gtagagagta cttttgtcct gtaaaattaa ggatttgctg
     6241 tataaatgcc ttatcctctt ctgcaaaaat catttgacct ttatttaaaa caggtattaa
     6301 agtagagatt ttatcatctt tatttttctt ttggtgctca ttaatgattt ctatatctct
     6361 gtagtaaaga acatcatcat tttttatttt aagttctaat tgattagcag ctgtaccatt
     6421 aaatgtattt ttttctatat aaacatgtgt tatatctgga tacactttta ataaataaat
     6481 catatgcaat atatatttat caaaatcagt tctagcattt attttggcta gttctgccaa
     6541 tctgccacat agtaaaccat tttctgattc acttccaaca agaaaagcac tatagtcatg
     6601 tttagaacca cctgcagatg ctgggtcaca taataacatt gtctttttga atgtatgagt
     6661 ttctatacat tctctagatt aagttctgat tgttttaaac catttctcac ctattgaatt
     6721 tacatcattt tgtacctctt gtttaaaaga tgttgggttt tcgtaataac ttaaagccat
     6781 gtctaaacaa ttccaaaaac ttggccataa tataggaaat ttaatttctt tttcattttc
     6841 ccagtaaaat tctttagcat cttcaagatg atttgtgttt ttgaaatcaa ataacaactc
     6901 tttgaaatta agccataaac cagaattaaa taattcatct acattatcaa ctaaaactcc
     6961 tttttcacat ttaaatttcc aagttggtaa attctttagc ctactataga aacattcttt
     7021 atgttgtaag gttcctaatg ctataaaagt tgttcctctt tttataatct taccatttct
     7081 tattactggt ttttaagcag catattttac atcatcagaa aattttttaa attttttctc
     7141 tctagcatct tctgttctaa cattttcttc tgactgataa tcatcaagta tgataagatc
     7201 tggacgatta ttattgtatt tttttcctct cattggtgat gaagatgata ttgcttcaat
     7261 aaaggtttta ttagttaatt ctaattgagt actattgcaa atatattttc tatcattatc
     7321 atttaagaga actccaaatg cttttttaat atactcattt tcaagtaatg cattttttat
     7381 atcttttacg aatttttctg ctgtagagcc tatatcagag caaattaaag tatatgtctt
     7441 atgtttataa caatgtgacc atacagttgt tgctaaatct ccaaatgcac ttttacctgt
     7501 tcctcttgga taaattcttc ctaattgctg tgaaccatct ccaattatag actcttgtat
     7561 atcttcccat aactcatgat gaactttagc tataggagca gcaacattat cttcttttgg
     7621 taaatatgtg tcttgtataa aatacataca gaaaaattct aaatttattt ttccaagctg
     7681 ataagctagg ccattaactc cccaaagatt gcttgaatta gtttttataa tctcatttgc
     7741 ttctttttca gcttctctta tagttgctcc atttcttata aaggtctttg ccatataact
     7801 aaatattaat tgtatatttc tttctaaatt atccactcgt atttacttcc tcttaattct
     7861 tctatttttt taggttcagt gagtaataca attaataaaa taaacaatgc aaattttttt
     7921 ctaataaaat caccctcttt gaaatatgtc tctggttttt aatttttgct agaaaatttc
     7981 tagaactcat tttctataaa taatactttt ttgaaataga aggatacccc ctatactgaa
     8041 aaacagttat ttataattat tttaaacata attaaagggc ataattctat gaatttgccc
     8101 cactaaaatt aacttaagca tatatattgc tcttcttagt tactctcatg gctctttatt
     8161 aatctatcta atgttggtct acttactccc aattctttag ccaattgagt cttattaagt
     8221 tctctagttt tatatctatc ataataacta ccaaagttgt ctatcttaac ttctttacgt
     8281 cctttatatt taccctgttc tttagctata gagatacctt ctcgttgtct ttctaacatg
     8341 tttgttctct caaattcata tattgcgcca agcattgtaa gcataagttt tcctgtaggt
     8401 gtacttgtat ctaaattttc tttatcacta attaacttta catgtttctt ttctaagagc
     8461 tcaactattt ctaataagtc tttagtactt ctagcaagtc tactaaagtc atgtacaatt
     8521 attgtatctc cttctcttac aaaatctaac attgcgttta gttttgatct tttagtatct
     8581 ttggcactta ttttctcctg atacattttt tctacattat gctttttcat tgtaactaat
     8641 tgtctatctt cattttgttc tgctgttgaa actctcatat aacctactac catctttgat
     8701 acctccacct atgtttactc tataaataat attatagcat aaatgtaaaa ctagagttaa
     8761 agatttgcca tgctttttgt aaattaattg gagttttgat tttattttta catcagaata
     8821 gatagtttgt aatgtaaatt tgtaccagca tactttacaa tatgctctta ggaaagatat
     8881 ttcttgttta tagattataa tatttctcta gtatctttat ttttataatt tcaaaaataa
     8941 aaattaaaat tacagttatt atcatatttc gtgtactatt ttctataata ttataactaa
     9001 acaatgcaaa aacaatgatt aataaaatta cgtttatagt gccagaaatc aagaatgatt
     9061 ttttcttatt attcatatat ttcctcctag ttatttgcta gttttatacc atctcctact
     9121 ctttcagtat atacaacttt tttaaagtta gatgagctat aaaaatatgt aacatattca
     9181 tatctgtatg gaacaggaac attagcatga ctgtttctaa tatcagttat ttgtctccta
     9241 tagtatgttt ttgtatatac gcctccatat ttagtaatgt catatacttt ttctctataa
     9301 atactctcag ctaaacctaa taaaaaagat gaccctccag aaagataacc taatccccat
     9361 tgaatagttt gtataattct ttctcttgta taaggagaac ttaatttgcg tgatttttga
     9421 cctagatatt taacattttt ccaactacca ggtgtaactt ttgttgaaac ataagtgtta
     9481 ttgccttttt tatccacaat catttcattt tcattgctat taataatagt aaattcgttt
     9541 tcattaacaa gttcttgttt atattcattt tcttcaaaag tagatgcata aaccaaatta
     9601 tatccactag ataaaacaaa tattcctgcc attaaaaaac ttattatttt tttctttaac
     9661 ataatatttt actaccttta cttaaatatt tattctataa acactgtcat ataacctgtt
     9721 agttatatta aaaataatct ttttatataa cttcacaatc aaaccttgtc ttatttcata
     9781 atagttttat ttaatatcat ataatttaga cctccttata agattattta tatatataac
     9841 gaataataaa agtaattttg gacaaaataa aaatcttttt attttaagaa tgaaaaattt
     9901 aaccctctaa gaaaactcaa ttaagagcga ttcatagagg gcttattatt atattatata
     9961 actttatata ctttttattt ttatatgctt caatttaact tatttcaagc atattcaagt
    10021 acttctcatt aactttatat acatcctttt ctctagataa aatctctatt tcttcaagat
    10081 tattaaacac ttccatcaac tggtcaacta attcgatttc ttttattcca gcctttaagt
    10141 atctaccttt attctttttg atatagttct ctaatgtcat agaataatta tctttactac
    10201 agatatattt taatatatgt tcttctgttg ctgatagagt tatatcattt tcaaataatt
    10261 cctcttttaa tatactctct aaagacttcc tatcatgcat atcacgcatt tcattgatag
    10321 attgtattat agactttagc atgaactcta taaatacagt taaattatat cctttattct
    10381 ctacatctag tatagcctta taatacttag ttctgttttt agatattata tatgatattg
    10441 aaaactcttt aaaagtatca tatcctttat taattaaata tagatatgat aaggctcttg
    10501 atgttcttcc attaccatca ctaaaaggat gaacatatac aaagtagaaa tgaattattg
    10561 tactctttac taaatcattt actttactat tttccataaa aattattaat tcatccataa
    10621 aattatatat tttcaaagga tcaagtcctg tatgtataat ctcacctttt gaatcacata
    10681 catatacttt ttcagttcta tattcatatt cttctgaatc taaacagttt tcacccaata
    10741 tatgatgtaa attatatata aatttgtgac tatataattt atctaaatta tctaatccat
    10801 attctaaggc tctatgatta ttgtaaatca tatactcact tttattactt ggtttcatct
    10861 ttcctctaat tatagatttt gcaatctttc tagttgaaaa ggcaccttct attacactag
    10921 aataataaga ctctaaggct aaatcatttt tatcttctat tattttaaat atcttgcttg
    10981 atgttaatct atctatttca tcttttgaat ttaatatgtt atcagtttca tgatagtata
    11041 gttctttatt atcaaaactc tttaaggaaa gtataatttt atctccagta gagtatttat
    11101 ttttgtattc ttctattgtc agtttaatca ccaccttagt ttatctttat ctattaatat
    11161 taacaaaaag tattagtttt tacactattt tt
//
LOCUS       DAMKPG010000108        11158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMKPG010000108 DAMKPG010000000
VERSION     DAMKPG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.113"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            59..457
                     /locus_tag="PC621_003107"
     CDS             59..457
                     /locus_tag="PC621_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4110244.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            445..1383
                     /locus_tag="PC621_003108"
     CDS             445..1383
                     /locus_tag="PC621_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF4110245.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1767..1991)
                     /gene="secG"
                     /locus_tag="PC621_003109"
     CDS             complement(1767..1991)
                     /gene="secG"
                     /locus_tag="PC621_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF4110246.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2109..2615)
                     /locus_tag="PC621_003110"
     CDS             complement(2109..2615)
                     /locus_tag="PC621_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110247.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2700..3128
                     /locus_tag="PC621_003111"
     CDS             2700..3128
                     /locus_tag="PC621_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4110248.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            complement(3248..4540)
                     /gene="eno"
                     /locus_tag="PC621_003112"
     CDS             complement(3248..4540)
                     /gene="eno"
                     /locus_tag="PC621_003112"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF4110249.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(5128..6660)
                     /locus_tag="PC621_003113"
     CDS             complement(5128..6660)
                     /locus_tag="PC621_003113"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF4110250.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            complement(6669..7412)
                     /locus_tag="PC621_003114"
     CDS             complement(6669..7412)
                     /locus_tag="PC621_003114"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF4110251.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            complement(7438..8640)
                     /locus_tag="PC621_003115"
     CDS             complement(7438..8640)
                     /locus_tag="PC621_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF4110252.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            complement(8862..9869)
                     /gene="gap"
                     /locus_tag="PC621_003116"
     CDS             complement(8862..9869)
                     /gene="gap"
                     /locus_tag="PC621_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF4110253.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            complement(9929..10951)
                     /locus_tag="PC621_003117"
     CDS             complement(9929..10951)
                     /locus_tag="PC621_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF4110254.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
BASE COUNT         3463 a         2026 c         1308 g         4361 t
ORIGIN      
        1 taaaatttaa taacttatat aaatttaact aatttatata aaatcttaag gagttgccat
       61 gataaaaaaa atagagcatg tggtcattac taataataat atagaaaatt atatcaattt
      121 ttataaaaaa tttggttttt catctaaaga aatgagcgaa aagtataaac ttaaggctag
      181 aaatgttaag ataaatctta acataaaagg ttataaattt ttaccttatg ttaaaaattt
      241 aaaagcaagt agagataact tatgttttaa aataaacaca aatattgatg agccaaaaaa
      301 atacttagaa caaaatgaga ttcaaattaa gcgaggaatt gtagaatgta caggtgtctt
      361 tggtaatatg aaatctattt atttgaaaaa aactaatgga aatttaattg aattatcaaa
      421 tcatagaatc acggagagta aaccttggaa aatttaatcc tatcaataaa tgttgttctt
      481 cctttgtttc tcacaatgtc acttggatat gttctaaaaa aattaggtct atttgatact
      541 catactttaa ataaaatgaa taatgtctgc ttcaagtctt tcttgccatt attattattt
      601 tttaatatat acaatacaga tttagatggt tctcttaatt ttaagcttat ggctgtagct
      661 gttataatta ttttttcaat cttcttaatc ttagtcttta ttattcctaa aattgaaaaa
      721 gataataaga caaggggtgc attaatacaa ggtgtatttc gtagcaattt cgttatattt
      781 ggtattcctg tgacaatttc actatttggc gataagagta ttggagtcac atctttatta
      841 attgcagtta ttgtacctat gtttaatgta ttatctgttg taattcttga aatttttaga
      901 ggtggcaaag taaatataag acatatgatt aaaagcgtct taacaaatcc actcataatc
      961 gcctctttta taggattgac aatgctaatt ttaaatatta atatacctac atttttacac
     1021 aaaactattg gtgatatttc taaaatagct actcctttat cacttataat tctaggcgga
     1081 tcttttagtt tttctgctat aaaggggcat ataaaaacca ttatattggg cgtatgtgga
     1141 aaacttatac ttgtaccttc tatattttta ccaattagca tttttcttgg atttagaaat
     1201 gtagaattaa cttcacttat gataatgtta tctgctccaa ctgcagtttc ttcatttaca
     1261 atggcacaac agatggattc tgacagtgaa ttagctggac aaattgtagt atttacttct
     1321 ggattttcta taattacagt atttttaata atatttattt taaagcagaa taatcttatt
     1381 taatgatatt tgataacaca aattggtatt atgagaaaca tatataaaat tgatttaatt
     1441 gcgtaaaaac atattattat actattttta ctatataata taactttgtg tatattatct
     1501 cattaaacat ggtgaaagta gtgttgctat atatttttta gtttgaaaaa aatatataaa
     1561 tataaacaat atataaatac aaataataaa tgccaacaaa tagtcaatct ataaaataaa
     1621 aactggacta atcatataaa atcattagtc cagcttttat tttacgtact ttttatgttg
     1681 atgtactttt attaaaaact aaatttaatt tttaaatatc tagcaaatgc actaattaaa
     1741 taatggccag ttattcaaca atatcactac ttcttaacta taagtaatgc caatgcattt
     1801 ataaaaaata atactgctgt tatctttgta actctagcaa gaaatgcttg ttttcctttt
     1861 ggtttaaagt aagcttgact tccttctcca ccaaattctg aaggtaaagc attttttgaa
     1921 tcttgaggca ttatgctaat tactagtaaa atccctagca ctacttgcac acccattaaa
     1981 atgttgctca ttattgcccc tcctatctat ttattactta aatttcatta ctctttagtg
     2041 tatcacaata atatagtcat ttcaagacta ttgaagtata aataacaaac aaaaataata
     2101 tgcaattact atatcttttt tgaataacgt acttctaata atttttttcc aatatgtatc
     2161 tctttctcaa aatcatcaca agtaaaacca ttttttttat agaaatttat agctctagta
     2221 ttgtctttta gaacccatat cgttatatat gaatatccta aattgcttaa tctatcaatg
     2281 caaaattgta atcctttttt tgtcacagat gttccccaaa aatcgggatg gaaatatata
     2341 gaatatattt ctccaacaca ttttggagca ttatcctctt gacttctatt taatgaagca
     2401 aaaccagcag gtataccatt cactttaaaa agataatatt cctcttcagc ttttggaata
     2461 acactttgaa aaacttctgc tcttttctca gaagtgaatt catctattat cttatctgga
     2521 ataattcctt tatacgcttt tctccacgaa caagaatgta taaaacccat ttctatagca
     2581 ttttcacttg tagctcgtat aatctctaaa ttcataaaaa caccatccta ttgacaaatt
     2641 aatacgatta tcgtactata ttagtgcgat aattgtatca tatatggagg taaaagtcaa
     2701 tgaatcctgt aaaagagcta aacaacctgt ggcatattgt tagtatgcag atgaaaacag
     2761 aaatgtgtag tggtaagtat cctactattc atggtatttc tttagtagaa ttaagtattt
     2821 tagaaatagt tgaacagtat cccaatagta tgatgaaaga aattagtgaa attttagaat
     2881 tgccaaaaag cacattaaca agtgctgtaa aacgtcttga attaaaaaaa tttattaaaa
     2941 agaatccctg tattaaagac aaaaggaggt ataacttagt cttaactgaa ttaggtttaa
     3001 aggctcaaca agaacataga gacatagaaa taattgtatt tagaaatctc ttacatcaat
     3061 tagataaaga tgaaatagaa acttttattc gtattttttc taaagctgtg aataaaagta
     3121 agaaatgaaa tataaaaaaa ggataagtga attgaaaaat caactatcac ttatccctta
     3181 taagataaat ttttatcttt tatgttatct ttttaatctt taatgccata tttttaatct
     3241 tcaataacta tttttttaaa ttatagaaag attttaatcc acagtatcta gcttgttctc
     3301 caaccatttc ttctattctt aataattggt tgtacttagc aactctatca gttcttgatg
     3361 gagcaccagt ttttatttga ccagcattta ctgcaacagc taaatctgct atagtagaat
     3421 cttcagtttc tcctgatctg tgagatataa cagcagtata tcctgctctc ttagccattt
     3481 ctatagcatc taaagtttca gttattgtac ctatttgatt aactttaact aatatagagt
     3541 tagctactcc attttcaatt cctttttcta atctctctgt gttagtaaca aataagtcgt
     3601 caccaactaa ttgtaattta tttcctaatc tttcagttaa taacttccat ccgtcccaat
     3661 cttcttcgtc taaaccatct tctatagtta ttattgggaa gttgtttgac caatcttcat
     3721 ataaagcaac catttcagca gcagttaact cttttccttc tcctgctaaa acgtattttt
     3781 tagtttcttt gttgtacatt tctgtagcag caacgtcaag tcctaacata acatcttcac
     3841 ctggcttata tccagctttt gttatagctt caactattaa ttctaaagct tctctgtttg
     3901 aacctaagtt tggagcaaat ccaccttcgt caccaacacc acaagctaat cctttttctc
     3961 ctaaaacttt ctttaatgaa tggaatactt ctgctcccat tcttaaacct tctttaaagc
     4021 aacaagctcc tactggtaat atcatgaact cttgaacatc aacgttatta tcagcatgct
     4081 ctccaccgtt taatatgttc atcattggaa caggtaattg tttagcattt actccaccta
     4141 aatattggaa taatggtaaa cctatttcat cagcagctgc tctagctact gccattgaaa
     4201 cacctaatat agcattagct cctaatttac ctttgtttgg agttccatct aattctatca
     4261 tgattgcatc tatagctggt tggtcaaatg catccattcc ttctatttca ggtgctatta
     4321 tttcatttac gttagcaaca gctgtttcta caccttttcc taagtatctt cccttatctc
     4381 catctcttaa ttcaacagct tcaaaagctc ctgtagatgc tcctgatgga actattgctc
     4441 ttcccattgc tccatcttct aatactactt caacttctac agttggattt cctcttgagt
     4501 ctaatacttc tctagcatat actaattcaa taactgacat ttgtatatct cccttcgatt
     4561 ttctattagt taaattttca tttactgtaa acatccgtct actattttaa tctactttct
     4621 atattatagt acataaagtt gcttttgaaa attaggtaca aatttatttg atgggtaaat
     4681 ttgtaaaagt tcctattttt attttatttt ttataatatt ttaatttata taatataaaa
     4741 aaacttgtct aaaatccaaa accaatctta aatacacaaa tattataact agatttaaaa
     4801 cattaaacat aaaatgtaca actaatgatt ctaaaatttt tgcttatata atatatatta
     4861 attattatta tttatattgt tattgtctat cttgttatta tttatatcat tatttacatt
     4921 attatctata ttataatttt ttattttata aattgtctat cttgtaaatt ttataaaata
     4981 taccttcatc tacaccttac aatatccaga acttacaaaa tctaaatctt atgtcataaa
     5041 gtctaaactt tacaaaataa acctaaactg cattattttt aaatttatat tgtagtttat
     5101 atattattct ctacttatca gaatcgacta ttttgaaata agtgaatgac cagtcatttc
     5161 ttctggcttt tctaacttca tcatatctaa aacagtcggt gctatatcag ataatcttcc
     5221 tccatccaat aattttgcac tttcatattc ttgaccaact acaataaatg gtactggatt
     5281 aatagagtga gccgttacag tttttcctgt ttccgggtct aacatatatt cagcatttcc
     5341 gtggtcagca gttattattg cacttccacc taactctact attttatcta tcaattttcc
     5401 tacacaagta tctacagttt caacagcctt tatagccgct tctatacttc ctgtgtgacc
     5461 taccatatca ggatttgcaa agtttaatac tataaagtca aacttatctt ctcctaattt
     5521 atctaaagct ttatctgtca attcatatgc tgacatttct ggttttaaat cataagttgc
     5581 aacctttgga gaaggtatca atagtctttc ttcaccttta tttggctctt ctactccacc
     5641 attgaagaag aaagttacat gtgcatactt ttcagtttca gcagctctaa gttgagtttt
     5701 tccattttta gctagatact cacctaaagt gttggctaaa gattgaggtc caaatgctat
     5761 atgcacattc tctatagtta tatcgtactc tgttaagcat acaaagaagt ttttaatatc
     5821 ttctctttta aatccatcaa actcttcaca aactaaagct ctagttattt gtctagctct
     5881 atctggtcta aagttgaaga atataattga atcattttcc tttatacttc caactggttt
     5941 atcatctttc ataataactg ttggcattat aaactcatca ttttttccat catcataaga
     6001 gttttgaata gcttcctcaa tagaatttgc tttttcacca atacctctaa ccatggcatt
     6061 gtatgcaagt tctactcttt cccatctctt atctctatcc atagcgtagt atctaccaga
     6121 aactgtagca aattcaccta ctccaatttc atccatacta gcttgtactt ctttggcata
     6181 ttctaatgca ctttgagggt cagtatctct accatcagta aatgcgtgaa catacacctt
     6241 ttgtacgcct ttatcttttg ccattttgat tatagcctta agatggtcta tatgtgaatg
     6301 tactcctcca tcagacaaaa gtcccattac atgaagagaa ttttcttttg cattatccat
     6361 tgcttcacat aaaactttat tagcaaaaaa gtcaccatct tttatagatt ttgttattct
     6421 agttaaatct tgatatacta ttctacctgc acctatgtta gtgtgtccaa cttctgaatt
     6481 tcccatttga ccatcaggta gaccaacatc aagaccacta gcatttatca atgtgtttgg
     6541 atactctttt tttattctat ctaaattagg agttttggac tcagcaattg catttccctt
     6601 aacatcctta ttgtatccaa acccgtccat aataattaaa gcaactggtt ttttcatcat
     6661 ttcactcctt agaaattaac aaggtctagg taatcattac tagctaaact agctccacct
     6721 actaaagctc catctatatc actttgaccc attatttcgg ctacatttga aggtttaaca
     6781 cttccaccgt attgtattct aacttcatta gctaattctc catataatcc ttttataact
     6841 tctcttatat aagatataac gtcattagca tcttcagcag ttgcagtttt accagttcca
     6901 atagcccaga ttggctcata tgcaacaact acttttgata aatcatcttt aagaacattt
     6961 tctaaagctt tttcaacttg aactttacaa acatctttag tttttccagc ttctctttgt
     7021 tctaaagttt ctccaacaca taatattggg tctattccta cttctaatgc ttttaaaact
     7081 ttcttgttta cagtttcatc agtttcattg aagtattgtc ttctttctga atgtcctatt
     7141 acaacatagt ccatatctat ttcttttaac ataagaggtg aaacttctcc tgtaaatgct
     7201 cctttttctt caaagtgcat attttgagca cctatcttta tatttgtacc cttagtagct
     7261 tcttttaaat cctttaataa agtaaatggt gcacatataa cagcttctac tttatcagaa
     7321 tttactttat ctttaacttc atttacaaat tctaaagctt cttttattgt tttatgcatt
     7381 ttccagtttc ctgcaattat aggttttctc attttacaat ctcctctata tattagttta
     7441 tttattatct aatgctgcaa tacctggtaa ttctttacct tctaaaaatt ctagtgatgc
     7501 tcctccacct gttgatacgt gagtcatctt gtcaccaaat cctaattgat taactgctgc
     7561 tgcactatct ccaccgccaa ttacagtagt tgcatcagtc aactctgcca tagctctagc
     7621 tactgataat gtaccatttg caaagttttc aaattcaaat actcccattg gcccattcca
     7681 tactacagtt tttgattctt ttatagtttt tacaaagttt gctatagtct taggtcccat
     7741 atctagtccc atgtaatctt cttttacatt agcatcttct gttactattg gagtagcatc
     7801 tggtgcaaag tggtctgcca taacaacgtc tactggtaat aataagttaa cacccttatt
     7861 attagctttt tccatcattt ctttagcata ttcaacttta tctatttcaa gtaaagaagt
     7921 tcctacttca taaccctgag cttttaagaa tgtataagcc attcctccac ctattattag
     7981 attatcaact ttttctaata attcgtttat aacagctaat ttatcagaaa ctttagctcc
     8041 tccaagtata gctgtaaatg gtcttacagg gtttgcaaca gcttctccta agaattttaa
     8101 ttctttttga attaagtatc cacaaactct ctcttgtaaa aactcgcctg caccaactgt
     8161 tgagcaatgt gctctatgag cagttccaaa tgcatcattt acaaatattt cagcaagtga
     8221 tgctagttct ttagagaaat tctcttcgtt tttagtttct tcttttctat atctagtatt
     8281 ttctaataat actacatctc cgttttccat tttttcagtt gctttctttg cattttctcc
     8341 tacaacatta tcgtctgctg caaatacaac ttcttttcct aacatctcag ataatctctt
     8401 tgctactggt gctaaagata attctggttt agcttctcct tttggctttc ctaagtgaga
     8461 gcataatata acttttgccc ctttagaaat taaatattga atagttggta aagctccgtt
     8521 caatctattt tcatcagtaa ttacaccatc ttgtaatggc acattaaagt cacatcttac
     8581 taatactttt tttccgcaca catcaatatc ttctatagtt tttttgttaa gcattgacat
     8641 aaatatctct ccttttaata aagtaatttt ttattgccaa gtataatata gtacatactt
     8701 ttaccctatt aaatatatca tattatcata tttttatata tagtatgtaa cattatttag
     8761 gtgtatttta tatatttttt cttaaaaaaa gtcctgagtc cgtagtatta aactacaaac
     8821 tcggactttg tcaatctact tttctatttt ctcaaaactg actattttgc taattgtgca
     8881 aagtatccta aagttcttat taattgagaa gtataagaca tttcattatc ataccaagaa
     8941 acaactttaa ctaattgttt tccatctact tccataactt ttgtttgagt agcgtcaaat
     9001 aatgaaccgt agcttattcc gattatgtca gaagatacta acatttcttc agtatatccg
     9061 aaagattcgt ttgaagcttc tttcatagct gcatttattt cttcaactgt aacatttttt
     9121 cctaatgtac aaactaattc agttattgaa ccagttacaa ctggtactct ttgtgctgcc
     9181 ccatctaatt ttccttttaa tgaaggtatt actaagccta ttgcttttgc agctcctgta
     9241 gtgtttggaa ctatgtttcc agcagcagct ctagctcttc ttaaatcacc ttttggatga
     9301 ggaccatcta aagtattttg gtcattagtg tatgcatgta tagtagtcat taatcctttt
     9361 tctataccgt atttatcatt taatacttta gccattggag ctaaacagtt agtagtacaa
     9421 gaagctcctg atataactgt ttcagaacca tctaatatgt cactgtttgt gttgaatact
     9481 attgttttta aatctccagt agctggtgca gatataacaa ctttcttagc ccctgcttgt
     9541 atatgagctt cagctttatc tttagatgta aagaatcctg tacattctaa tactatatct
     9601 actcctaatt cagcccatgg taggtcagca ggatttcttt ctgctgttac ttttatttct
     9661 tttccgttta ctacaaaagc accttcttta acttctattt ctccgttaaa tcttccttga
     9721 gctgtatcat atttaaataa atgagctaac attttagcgt ctgttaagtc gtttattgca
     9781 acaacttcga acttatcttg ttgctccatc atttttctta atgctaatct tcctattctt
     9841 ccaaatccat ttatagctac tttaaccatt ttcgctacct cctataaagt aaatgtatgt
     9901 tttctagctc tgtgctaggt cttgactttt ataataatga caatatctta taagcacttt
     9961 cttcatctgt tacaagaacc atgtttttat gcatatttga tatagctaat aaggcttcta
    10021 ctttttcagt ccctgcaaag attgcaatag tttcttttac ttgtttaact gtttctaaat
    10081 caattccaac agtgttcagt ttgtaaacta tttctccatc tttgttgaaa taatatccaa
    10141 atgcttctcc aactgcattt ttagataata tctcatcaac cttgtcttct ggaagttttc
    10201 ttctctttgc catgacatct gctctaccta tactaaaaac taaaatgtct gttcttttaa
    10261 tatactccaa agttgtctta atctctggca cttggctaag agttttcatt gcactttcat
    10321 ccaactcatc tggtatatgt aaaagtttgc aattagaatg taatttctta cttattgaag
    10381 caactatatt gttagattgc atctctacat cagttcccat acttcctcta gctggaacta
    10441 ttgtagagtt tggatatctc ttatcagttt taataacgtt tgaaaactcc aacatagtgc
    10501 ttcctcctgt tattgaaacg atatctccat cttttaaaat gcctaagaaa tactcgcatc
    10561 catatctagt tacatctttt agtaaacttc cattttcatc acagcttcca ggaactagta
    10621 ttaccctttt tattccaagc ttttctctga ctctatcttg taacttagac aatcccatga
    10681 tgtcactcat gatgtcctta agtttatcta acaaatcaga accttcttta gtgatagtca
    10741 tcccagatac agctacgtct ataagtcctt gttcttttaa aaactcagtt tctgacctga
    10801 ttattctttc actaatctct aatacatttg ataatgttct cctacctatt ggttcactta
    10861 aagatatttg tcttaaaata gagtatcttc tttccataag ttcaattgcc tgtggaatca
    10921 atttcttttg aatttctaat aagttcttca taacatactc cttattgctt tcctttcggg
    10981 actttttttg tccctagtgt ttatttaagt cccacaagat atttttattt tataggattt
    11041 gcgtatagat ttcaatagtt tttcaaaaac ttttttattt ttttatttta taaaatattt
    11101 ctccaccatt ttcaaatcac agtatttttt ctttttatat aaggcttttt atatatat
//
LOCUS       DAMKPG010000109        10971 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMKPG010000109 DAMKPG010000000
VERSION     DAMKPG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.114"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..449
                     /locus_tag="PC621_003118"
     CDS             <1..449
                     /locus_tag="PC621_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110255.1"
                     /translation="KQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEKYINDP
                     FCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRD
                     RYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            914..2128
                     /locus_tag="PC621_003119"
     CDS             914..2128
                     /locus_tag="PC621_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110256.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2548..3069)
                     /locus_tag="PC621_003120"
     CDS             complement(2548..3069)
                     /locus_tag="PC621_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110257.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            3425..4222
                     /locus_tag="PC621_003121"
     CDS             3425..4222
                     /locus_tag="PC621_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF4110258.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            4332..4922
                     /locus_tag="PC621_003122"
     CDS             4332..4922
                     /locus_tag="PC621_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF4110259.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            4988..6685
                     /locus_tag="PC621_003123"
     CDS             4988..6685
                     /locus_tag="PC621_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF4110260.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGISLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     NVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            6899..7738
                     /locus_tag="PC621_003124"
     CDS             6899..7738
                     /locus_tag="PC621_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF4110261.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            8255..10111
                     /locus_tag="PC621_003125"
     CDS             8255..10111
                     /locus_tag="PC621_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF4110262.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    10194..10434
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    10500..10735
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10820..>10971
                     /locus_tag="PC621_003126"
     CDS             10820..>10971
                     /locus_tag="PC621_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF4110263.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYS"
BASE COUNT         4475 a          992 c         1863 g         3641 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag atttggtggc aaaataataa
       61 gaagaaatga aaagactaag tttgactggt taagtagaga taaagaacaa gttgaaaaat
      121 atataaatga cccattttgt ggagtagtgt gtagttgtgg ctttttttat gatttagttc
      181 aagggttaaa agaaattgag gataaagaaa acttaaaaaa agtgccactt gatataccta
      241 tttacataat atctggagat aaagacccca taggaaaaaa tggtaaagga gtcttaagac
      301 taagggatag atatataaaa ttaggagtaa aagatgtaac ttgcaaactt tacaaagatg
      361 gaaggcatga attgttgaat gaaataaata gagaagaagt ttttgaggat ataatttgtt
      421 ggttaaacaa taaaatagaa atattgtaat aaaaaaatat aatgatatat ttgctttaaa
      481 tttatttata tataatattt gaaaagtgga ttatgtatat gcaaagtaga aaagagagag
      541 cctagaagtg attttttgct ataatgccat ttattgaata ggtgggtgtt attataaata
      601 aattattaac actcagtatt attatcattg tatatggaat ttattttatt taaaattcct
      661 tgaaagttta aatatagtaa gttgtagtaa agagaaaatc taaatattta aatgctattt
      721 aatgtgaatc tattaattag gttatactta aacttaatta atagatttta tatttcaagc
      781 caatgagata gggttttaga ttaaaatata accaatggtt aaattacaga cattttgtta
      841 actttccctc gatttactag taaatatttt ttttatatac ttaaattaca acagatgtac
      901 tagggaggta tatatgcaaa ttaaaatagg taaagtcata cagcgtctta gaaaagaacg
      961 aaatttaact caagaacaat tagcaaagtt tataggtgtg tcaacaccag cagtatctaa
     1021 gtgggagagt ggaaattctt acccagatat agaattattg cctttattag cagacttttt
     1081 taatgtgtct attgataagc ttttaaatta caagattgat ttaagtgaag aaaaagtaat
     1141 gaaaatttat aaagaattag aatcaggctt tgctagaatt gaaattgatt tatcaacaga
     1201 agaaccaaaa gaagaattta gacaagactt agaatctgta aaaaagcttt ctaatatgta
     1261 tatagagaag tatcctaaaa gctatttatt gaagctaagg atatgttcgc tgtatcagat
     1321 gtattcatat aaatttggta agagtgaact taatgacaga gtaaaagaaa caacgaatat
     1381 tcttgaagat atagtgagaa atacagatga tatccaaata aaagaaacag ctcttattat
     1441 attatcaaac gcatattgta tgttggaaga ttatgaaaag gcagagttgt atttaaatat
     1501 gatacataag tcaatagggg atacaagtgt taatttagct atgatatatt taaagcaaaa
     1561 tagacttgaa gaagcagaaa tattgcttca aaataaattg tttagtaata tatttaatat
     1621 aagcatggat tgtaagggaa taattaatgt atacaaaaat caatacaagg aattaaagaa
     1681 aaaattggag aatagaaatt ttaataaaaa tgcaattgag agagaaatgg agtatataaa
     1741 aaataagttg ctgtggtatg caaatctttc tttagaaata aaaaaaatgc tcagtgaaga
     1801 taaaggtgca ttttttagta tgtatgtgga ttatatggaa ttatctctaa tttttttatt
     1861 ttttaacatg aaagaagaag caaaaaaagc cttatatagt ctaaaagaaa tattagaaaa
     1921 atatcctata catgaaagtt tggatgtaag tcaaatgaga ttttttgata aggtagagtc
     1981 taaaaattta tatactttta atatatatac aaacttgtta attgtactta atgatgacag
     2041 ttataatgag cttagagaag aaccaatttt taaggatgta attgaaaaga ttttagatat
     2101 ggaaaaaatg ttgaaaaata aagaataagt aaaaatttaa tataggactt aaagattaat
     2161 caaattaaaa gaaagtgttt caaaataaaa atttgaatca ccttttttgt tggtcttaaa
     2221 ccgttattta gaaaaataga ggtttcaaaa tagaaatctg aattatctgt attattacac
     2281 aaaaatagaa acctaaattt ataaaaatag aataaagttt gttatataaa atgtaatttt
     2341 gtctataaaa tctcacatgt tttcttaact ttaagcgtga aaaagaggat gtatcatgaa
     2401 ttttttagtt catgatgcat ccttatttgt atgaaatcca atatatcttt attatttaaa
     2461 caatggaaaa gagtcttatt tctattttga gacatctctt ttatttaatt atgaagtttt
     2521 atacagttca ataaatagga attatattta gaaactcatg acattatttc ttagatagtg
     2581 attaaattta tcatattcca ttttagataa cttttctggg taagacatta caatcaggtt
     2641 atgcccattg aatttttcat ggtatggtgg atgaatgtgt tcataattat ttgtcttata
     2701 ttttactctt tcatagaacg ccttgcgata aattgaaatc tcatcaattg gaaggtcaat
     2761 ttctaaaata atagtttttc ctttcttatt taaaagctct aacgctcttt gtccatatct
     2821 attattgcgc atttctggaa aaatgcagaa atgttctaca tagataaagt tatctgtttc
     2881 ccaacataaa attatgccga caaactggtt tttatcataa attaaattga attgatattc
     2941 tttatttttc aaaatttctg tttgcaaata agattttctt tgttcatgaa atgggaaact
     3001 aattttatat aattccatag ccttgtaata taatttattt tcactggttc ttaaccgttc
     3061 aaatttcact ttactcctcc taatcacgaa aattccaatt tatttatgag ttcattttac
     3121 catagtcagt atttgcatac aatgtgtttt aagttgagtg tcatttcttt cttttattta
     3181 tgcttatatc tcatagacta agagataatt gcttaagaca taactaaaaa ctattatatt
     3241 aagctttgat aatctaaaaa cagtatattt atggactttt ctaggtctaa aagtaatctt
     3301 aataatttta gaagttacaa aaaagttaca taaatttgga aagctataat atacatatat
     3361 aatataaggt taaaactgta atgaataata ttaatttaaa atttgaacat ggaaggagat
     3421 tattatggag aaaagagaaa agaggataat aatatcatct ttattaagtg tatcaatttt
     3481 aatgggtttg gtaagtatat attctatatt aaataaagaa gacataattt taactgttaa
     3541 gggtcaagag caaaaagtat cttcctttaa gaaaacagtt gaggaacttt tggatgaaca
     3601 gggtgtaaag tataattctg aagataagat taatccaagt ttagatacag aactaaaaga
     3661 tgatatgaaa ataaaagttg ttaaggtaac taaaagtaaa aaagaagaga ttgaaaaaat
     3721 tccatttgat acaaagcatg taaatgatag taatttgtta aaaggaaaat ctaaagttta
     3781 tcaagaaggt caagaaggag aaaaaaaact agtctataat ttaacttacc atgatggaaa
     3841 attagtcaaa aaagtcttat caaaagaagt aatatctaag gagccaacta caaaaattat
     3901 aaaatatgga actaaagaaa aagtactaat agcatcaaga ggagcaaata taagaggagg
     3961 caaacatatg aaagtggttg ccactgcata tgcaggagat acaataacat ctactggtac
     4021 aactccaaga tggggtgtta ttgcagttga cccacgtgta ataccatatg gaacaaaagt
     4081 atatatacct aaactgggta tgacttttgt agcagaagat tgtggaggtg caattaaagg
     4141 taatagaata gacattttta tgaacagtga aggaaaagct tcaaattggg gaaggaaaag
     4201 tatagatata tatctacatt aaattagtga atagaataac tttacttgaa ggacaaaaaa
     4261 gtataaaata tgaattaact aaactgttag attattctta aaacataaaa atatatctaa
     4321 ggggggataa gatgaaaata gtctgcttag gagacagttt aacgtatgga tttggagtat
     4381 cacgaagtaa ttcatggaca aacattgtaa atgaagagac tcgattagaa atagtaaata
     4441 agggtataaa tggtgataca acaagtggta tgttagctag gtttaatgaa gatgttgtaa
     4501 aaaactctcc tgatatagtt tttataatgg gtggcactaa tgattttatt gctggtgcgg
     4561 gaaatgaaat tattaattct aatataatgg ctatggtgca tcagtcatat tcaaaaaata
     4621 ttatacctat aataggtata ccattaaaac cagatattcc aaatgttagg gaagattgga
     4681 gttgttttac tgattttaat atagtatctc aaaacttaga atcatatagt tattggataa
     4741 aaaaattttg tatgacattt aatactaatt tcgcagattt ttattcagaa tataataaaa
     4801 atatggaact agaaggatat aaaaaattat attttgatgg gttacatcca accaaagagg
     4861 gtcatagaat aatggcagac atttttatta attcaattaa taagtatata gaagagttat
     4921 agattacatt aaaatgcttg tttatgcgtc agttgcaata aatacataca gtagagaggg
     4981 gtttattatg gattatacta aaacttatga agagtggata aagggttcat attttgatga
     5041 agatacaaag ctagagttgg agaatataaa aaataatgaa aaagaaatag aggatagatt
     5101 ctataaggat ttagaatttg ggactgcggg tcttagagga ataattgaag caggaacgaa
     5161 tagaataaat aagtacacag ttagaagagc tacttttgga ttggcaaact atatattaga
     5221 aaacacaaca aaagaagaaa caagtagagg agtagtaata gctcatgata atagacacaa
     5281 gtctagacaa ttctgtatag aatctgcaaa tactttggca gcatgtggta taaaagcata
     5341 tatatttgat agtttgagaa ctactccaga attatctttt gctgttagaa gcttaaatgc
     5401 aatcgctggt atagtcataa cagcaagcca taatcctcca gaatataatg gatataaggt
     5461 atactgggaa gatggagcac aagttatgcc agagattgca aatgcaataa cagagaaagt
     5521 aaatagtata catgattata gtacaatacc aaccttaacg gaagctaata aaaatttggt
     5581 agttttgttg gacgaatcac aagatactaa atttatagaa gcagtaaaaa gccaaattat
     5641 aagaaaagat ttagttaaaa atgtaggtaa aagctttaag atagtttata cacctctttg
     5701 tggaacaggg aatgttccaa taagaagagc attgaaagaa gttggttttg aaaatataat
     5761 tgtagttcca gaagaagaaa atccagaccc aaattttgca gggcttgatt atccaaatcc
     5821 agaagagaaa aaagctttaa atagaggtat attactcgca aaagaaaaag gagcagacct
     5881 tgtaatagca actgacccag attgtgatag agtaggtgta gcagtaaaaa ctactacagg
     5941 tgagtatgct ttacttacag gaaatcaaat tggtggtatg ctgacacatt atataataga
     6001 aggattaaaa gaaaataaca aattaaaaga aaatcctaca ataataaaaa ccatagttac
     6061 atcagaattt ggagcagata tagctaaagc aaataatgtt gatgtattaa atgttcttac
     6121 aggttttaaa tttattggtg aaaaaataaa attatttgaa caaaataaaa atagaagtta
     6181 tgtatttgga tatgaagaaa gttatggtta cttagttggg actcatgcta gagataaaga
     6241 tggtgtagtt tcttcattgt taatatctga aatggcagca ttttactatt caaaaggtat
     6301 aagtttatat gatggtttaa tagaacttta taaaaagtat ggatttttca aagaacagac
     6361 tatatctttg actttaaaag gtatagaagg tgtagaaaag ataaaagaaa taatatctta
     6421 ttttagagaa aatcaaattg attatataaa tagtattaaa gtagttgata aaaaagatta
     6481 taaaaatggt atagataatc ttcctaaatc taatgtttta aaatactttt tggaagatga
     6541 atcttgggta gctataagac catctggaac agaaccaaaa cttaaatttt acatagctgt
     6601 aaaaggagct agtgatattg aagcagataa aaagattcaa ggtttgaaaa agtatataga
     6661 tgacatggtc gaaaaattaa aataaaatat tattatttta taagataatt ttgtaataat
     6721 atttatttag ctgaaaatta taatttactt tcattgagta gaatatggaa atgttagtag
     6781 aattgttatt ttcagctttt atttgtgtta tttttactta aataattata ttatagaatg
     6841 aatctgagta taattaatat tgtataatga aattaatttg atagaaaagg ggatatttat
     6901 gtttggtata gataagaaat tgattgaata tataattgca aaatccaatt acagtgaatt
     6961 aagttcaaag tcagcagaag tatctttctt cttaatgttg tccatatttc cttttttaat
     7021 atttacaata agttctatag cttatatacc gattcttcat ttaaataagt atatagcttt
     7081 gtttagaaat atgatgccag aaggtgcttt tgctgtgtta tcttcaatca tagtttctgc
     7141 aatagacaat agaaacctga aatttttagc agtcagtttt gtacttacta tgtggacatt
     7201 ttcaagagca gtaaaagctc ttattaaagg aatgaataga gcctataaag ttaaagaaac
     7261 taggtctttt tttaaaattt tatctatatc atttttattt acaataatgc ttttggtcct
     7321 tattttttta tctatgatat ttttagttta cggggaaaaa ataggttatt ttatttttaa
     7381 tcttgtagga ttggatgaga tatttataaa aatatgggat attctaagat acactgtagg
     7441 tataataaca ataatagtga tatttacact tttatataaa tacactccaa ataaaaagtt
     7501 aactattaaa gaatctgcgc caggtgctat ttttgctact tttgcttggt ttttagtatc
     7561 atttttatat tcttattata caaattacta tgctaactat gaagtaatct atggaagtat
     7621 agctgaaata attgtactta tgacctggat gtattttagt agttggtcta tcgttattgg
     7681 ctatgaggta aattcaagat tgtattttag aaagataaga catgaaatgt taaagtaaag
     7741 tatttaaaat aaaagagagt atttagtatt ataaatgtaa ttgaattaat aaagaaatat
     7801 tgacatttaa gttaaaaagt ctagaatttt tctgggcttt ttttattttt agtataactt
     7861 gacaaatttt atttaatggg atagttttaa tttataatca tctttgaaaa gatgactatt
     7921 gataagtaag gtataaaatt ggtaagtatc aaaaaatata ctaaataaaa attatagtcc
     7981 taatgagtta atttctgtat tttcaaataa tgcagtattt agtcatgtgt ttttatatgc
     8041 gcctgtattt ccaattacca atatattaga ataagctact gtaattagat ttagaaaaaa
     8101 gcgacataaa taattataaa caaataataa attacataaa attaaattta ttcacaagta
     8161 tatcttataa aatatgttat aattaataga aaattgtcat ataattaatt aagtagtatt
     8221 attataaata acattgaaag gtagtggtta gaatatggat atccataata aatatgtaaa
     8281 ttttataaaa aatatacctg tacctttttt atattgtaga attgtaaaga gacaagaaga
     8341 tatagaatat cgagtagaat atataagtaa gggtatgggc aaagtattac aattagaaga
     8401 aggtatttgt gataaaaata tactagatgt attgcctgta tttaagtcta aaaaatattt
     8461 caaagagttg ttctcaaatg aagtggattg tataaaaagg tacattccga cacttaaaaa
     8521 ctggataaat ataaaaaaac agataatagg ggattcatac ataattttgt attttggtaa
     8581 aattgtattt gattatagac agataatcga ttcctttgat aaaaaggaaa aagtagctta
     8641 tattaaagac gaagaaggca tatatataga ttgcagtgaa aatttaatac caatattaaa
     8701 taacaacatt aaaacaacga aagatatctt tggtaaaaat gatatagaag tatggggaga
     8761 gaatacagga aaattattta gagatgatta tagaggagga gtatctagta aaaaaagatt
     8821 tttacaaaat ctttttgaat atgaagaaac attttttatg gttgaaaaat attttttgta
     8881 tgatgaagac gaacttttag gtactatagg gataatagat aatataatat atagtggtta
     8941 cagtaataga aattataact caaaagattt aatgaagatg atagaacact caattccaga
     9001 gaatatgttt tataaagatg tgtatggaaa ttatattgga tttaattcag gttttttgaa
     9061 tttagcttgt atgaataaag aagaattatt agggaagaat agttataaaa tatcagaaga
     9121 agaagcttta ataaataaga tatttgaaag tgataagggt gtagttgaaa ataaaaaagt
     9181 agttacattt gagttaaatg taagtataaa tgatgaaaat aagtgtattg agattacaaa
     9241 aaggccgttt tttgatagct atggaagtgt tattggaata attggaacag caagggatat
     9301 aagtagaaga aaaagattag aagaggaaat ggataaaact aggatggaat tttttgcaaa
     9361 tttatcccat gaacttagaa cgcctataaa tttaatatca tcttcattac aagtgataga
     9421 aaaaaaagaa gcagatttaa tagaatcaaa tgatacttta aaaagaaatt taggtataat
     9481 aaaacaaaat ggaaatagaa tactgaggtt agtaaataat gttatagact ttacaaaaat
     9541 gcaatcagga tatttagatt ttaaaccaga agaatctgat ataattgcat ttatagagga
     9601 gatatgtatg tctgtagctg attttgcaag ccaaaataat atacaactta catttgatac
     9661 agaaatagaa gaattttcaa tgttatttga ttcagaaaaa ttggaaagaa taatattaaa
     9721 tttactttct aatggaatta agtacaataa gaaagatgga aaaataaata tatttttata
     9781 tgtgaaagat aatgtattta atatgaaaat atctgatagt ggaataggta taccaaaaga
     9841 aaaaatagat aaaatattta atagatttga acagattgac aatgaacttt cctacagagt
     9901 taaaggaagt ggtattggac tttcattagt gaaatctttg gttgaattgc atgagggaag
     9961 tatatcttta aaaagtcaac ttggtattgg aagtgaattt atagtttcat tacctgttag
    10021 aagtgaaaat aatattgaaa aatataatca taaaagagaa atcagcaatg aactaagtaa
    10081 aaaattagaa atagagtttt cggatttata agtttaaata taaaaaataa aattttaaaa
    10141 aagtattgac aattgattta gaagggttta aaataaatta caacataata aataaaaagg
    10201 cgatgaatag gaagagtaaa tctaattcga ttaccagaga gaagagtcca ttggctggaa
    10261 gactttttaa attagaggga ttgaaaacta cctaggagct actttctgaa gtcatctgca
    10321 ttagtagatt ttagtaagaa tagtcgttag ctgcgttaaa gtgtaaagtg agttttcatg
    10381 atatatgaaa actaaattag gtggaaccgc gggaataata ctctcgtcct tatgttataa
    10441 ggatgagagt tttttgttgt ttaaaaatat aaacagtaat cataataatt atagaaaata
    10501 taaaagcgat gaataggaag agtaaattta attcgactat cagagaggaa agttcacagg
    10561 ctgaaagact ttctaagttt gagaaattga aaactaccta ggagctgtgg tctgaagtta
    10621 ctttaaaaag ttttagtaag actaaccgtt aactgcgtta aagtgtaaag tggattttat
    10681 caataaatga taaaatcaaa ttgggtggaa ccgcgagtat agactcgtcc ctttatatag
    10741 ggatgagtct atttttattg caataaaaga ttgaaaataa aaaaaaatcg gaaaattata
    10801 aatttaggag gaaaataaaa tggaaaataa agtgaaaatt ggagtactag gatatggagt
    10861 tgtaggaagt ggacttatag atataataga taataataaa gaaaaaagaa gtatagaaat
    10921 agttggaatt ttagttaata atttggaaaa acataaggat aaaaaatatt c
//
LOCUS       DAMKPG010000110        10891 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMKPG010000110 DAMKPG010000000
VERSION     DAMKPG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.115"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            195..590
                     /locus_tag="PC621_003127"
     CDS             195..590
                     /locus_tag="PC621_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110264.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            633..2732
                     /locus_tag="PC621_003128"
     CDS             633..2732
                     /locus_tag="PC621_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF4110265.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(2957..8167)
                     /locus_tag="PC621_003129"
     CDS             complement(2957..8167)
                     /locus_tag="PC621_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF4110266.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(8182..10305)
                     /locus_tag="PC621_003130"
     CDS             complement(8182..10305)
                     /locus_tag="PC621_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4110267.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(10370..>10891)
                     /locus_tag="PC621_003131"
     CDS             complement(10370..>10891)
                     /locus_tag="PC621_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110268.1"
                     /translation="IENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEH
                     IANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIKNRKLSRLISDVSWSEFIRQLKYK
                     ANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREWICPSCNETHDRDINASINI
                     LKEGLRLITIQNK"
BASE COUNT         3472 a         2033 c         1281 g         4105 t
ORIGIN      
        1 catatataag ctatataaaa attatttcaa ctatataaac tacacatgta aaattcatgt
       61 aaatttacta atataaatca aataaaatta ctaaactctc taaactaatt ttgtaaaaca
      121 tactctattt ttaacatttg ttaccttata cataagtata aacaaaatct aactaaatga
      181 aaggataatg taaaatgaat attttaaaaa tgaaaaaaac aggaatattt ttaggaggag
      241 tactttttgg tactgtaggt gttaagatac tttcaagtga ctgtgtaaaa aaaacttgca
      301 ctcgtgcaac agcaggagca ttgaaagtta aagattctgt tcttgaaaca actacgaaaa
      361 ttcaagaaaa tgtagaagat attttagctg aagcaaaaga aatcaacgag caagaagact
      421 tatctacatc taaggtaaac gttgtacaag atactcttaa atgcgaacaa gaagttctta
      481 aaactgaaac tataaaaact gtagaagata ctaattctaa taacattaaa tgtgaagaaa
      541 tcaaagataa cgtaattgaa gaaaaaaata atacttgtac aacagaataa atttaatgat
      601 tattatgatt aataatatca ggaggaagta aattgaattt ttatataaaa cacgaaataa
      661 gaggtcgtat acgctttgac ttacaacttg gcaaattaaa caacaagcag tccgatactc
      721 tcttatatta tttaacttct ataaatggta ttaccaaagc aaaagtttac gatagaactg
      781 gtgatgcagt catttattac actggtgaaa gagaatacat aataagagaa atcactaaat
      841 tcagttttaa caatagcaaa atagaaaacc tagttcctga acataccagc agggaattaa
      901 acaattatta tagagacaaa ttagcaaata aactgctagt caaaagtatc tccaaaatat
      961 tttttccaaa gtccttaaga ttttttttaa ttggacttca ttctataaaa tattttaaaa
     1021 aaggtctctc ttccctacta aataaaaaaa tagaaatttc tgttttagat gccactgcta
     1081 taggtatttc tatttttaga aaagatatca atacggctgg ttcagttatg tttcttcttg
     1141 gtatcggtga gttattagaa gaatggacta gaaaaaaatc tatagacgac ttggctcaaa
     1201 gtatgtcact aaacattgaa aaagtatggt taaaaaaaga tgccactgaa atactagttc
     1261 caatttcaga gataaaggaa ggagatttag tcagtgtaac tatgggaaat atgattccat
     1321 tggatggagt tatagtatca ggagaagcaa tggtcaatca ggcatcactt acaggtgaat
     1381 cccttgcagt taacaaaaaa gaaggctctt acatatatgc tggaactgga atagagcaag
     1441 gcaatattgt tatgtgcgtc aaggaaaaag ctggtactac tagattccag aaaatagtaa
     1501 ctatgattga agaatctgaa aaactaaaat cttctgtaga gagtaaattt gaacatctag
     1561 cagatacatt agttccatac agctttttag gaagtatatt aacctatgca attactagaa
     1621 accctataaa atcactctct atattgatgg tagatttctc ttgtgcatta aaactctcta
     1681 tccctatttc agtactatca gccatgagag aatgtaacaa tcacagcatt gctgtaaaag
     1741 gtggaaaatt ccttgaaggt gttgcatgtg cagatactat tgtatttgat aagacaggca
     1801 cattaacaaa agctcaacca actgtttcag atataattac attccaaaat tataataaga
     1861 aggatatgtt aagacttgca gcttgcttag aagaacactt cccacactcc atagcaaatg
     1921 cagtagttta tgaagctgaa aaacaagggc ttagtcacaa agaaatgcat acagaggtag
     1981 aatatattat tgctcatggc atttcaacaa aggtcgatga taaaaaggtt gtaataggta
     2041 gcaaacattt tgtatttgaa gatgagaagt gtactatccc agacggtgag gaggataagt
     2101 acaacaatct atcagatgag tattctcacc tatttatggc aatatcgggt aaattatcag
     2161 cagttatttg cataaatgac cctttacgaa aagaagcaaa atatgtaatt tcaaatttaa
     2221 gagaatatgg aataaaaaag attgtgatga tgactggaga tagcgaaaaa acagctaaat
     2281 ctatagcaag taaagttggt gttgatgaat attattcaga agtcttacca gaagacaaag
     2341 ccaattttgt taaaagtgaa aaactaaaag gtcgcaaagt aattatgatt ggtgatggaa
     2401 tcaatgattc tcctgctatt tcagaatcag atgtcggaat tgcaatgagt gaaggtgctg
     2461 aaatagcaag agagatttca gatattacca tatcagcaga taatttgaat aatttaatcg
     2521 ttttaaagca gattagtaat aagcttatga aaagagttga tttaagttct aagtttatta
     2581 tcggctttaa tttaggtctg attttacttg gcgtaagtgg atttgttaga ccaagtacat
     2641 ctgccttttt acacaacgca tctactgttg gaataagttt aaacagtatg acaaatttat
     2701 tggaaaacac caatacttat aactactatt aatccccatt atataaaaca tatgtatata
     2761 aaaaatttat aattcatctt ttaaaaagaa actgtcttaa aaggagcttt tttctaatca
     2821 aaattatata aaacatattt tacaaatttt aaactcaact tatttctatt ttttacaaaa
     2881 aggagatgat tcaaattttc attttgaaac atcccctttt gtaaatttct ttaaaactta
     2941 tatattaatt atattttcaa caaataattg tattttcaac caatctttct tcatagcatt
     3001 ttaaatcatt ttgctttaga taattaaatt cagactctac ataaatacca cagcctacca
     3061 attcaattgt caaattaggt atattttcct ttagtatact agtctgctca taaataattc
     3121 tttccaataa atcaacttgt aatatagaaa aatgcttcat aatctcccca tcactatcta
     3181 tatctttctt gatttcatac caactctttg acaaaattcc cttcaaatca atatacccta
     3241 gactcttcaa tatatctata ctctcgctcc atgcatactt actaggttta tcaaagtatg
     3301 attctagttt cttcttctgc tcatttgtaa gtgaatcata agaaagcttc ttattaaaat
     3361 gtgccttcaa agaaatctta gccatagaat atggacttct accagagttt aatcctgcat
     3421 ttttgaactt ttcaattcct ttatcttcta ctgaagacca tattgagttt attgaatctg
     3481 aatcattgta tttaacatca agaccatgtg taatatctat atagttttgt ggaaatacat
     3541 attgataata gacatctttt ttatcatcat atagggagaa gtatccacta tcatataaat
     3601 gaatatgcac ctttactttt aaactatcct ctaaaatatt tcgtctaagg ttcgggatat
     3661 ttgcatacat ggttacatat tttatggcat caaagttttt atttaatgga acttcaaaac
     3721 taatttcatc tcctgtcaaa agctttttca cttcttcttc attacaatca tatagagaat
     3781 atgtattttc aaatcccaca cgtgatttcc catctaaact gcctgttaat gctaccttca
     3841 aaatatggtt atccgttttt tgccattggt aagcatcttg gtcaagaaac tctctaatct
     3901 gtctattttg ctctttcgcc tgatttataa cctcgttttc atcttctgta atacctacat
     3961 ggcatctatt taagatattt actatattgc ttccaaatgg aaacgtccat agttgaaaag
     4021 ctgctgcctc aacctgacac aaatatctaa cctgatttaa ttcaatttga tggataagtt
     4081 ttttctgata ttcattcatc tgattaccac tacctgacat ttgataaatc aattctccca
     4141 ttgcaagatt atcttttttc ttatcaatct tatcagtaat gataatttga gtttccatca
     4201 tatcctgtac ttttttaaat attatagaaa tctcctctga attttccatt tttaagcttc
     4261 caaactttgt gcttaattca gttaaaactt ccttcatctt taatttactt atctcatttt
     4321 caaaaatatc ttgattttta actgtactat cgaatttttc tacaaatggt ataacagatt
     4381 ctttcaaagt tttatcttta aattccacca tttgagtatt caattcatct aatttcttaa
     4441 tattattttc tttatcttta attgcctctt taactacctc tattttactc tcaaaactag
     4501 attttacatt aagacaatta ttaacaccac taatcgcagt atttataaga gaacaagcag
     4561 aacttccaat actcaatgca tttttaattt tagatgtctt ttgctgtctt tcaatagctt
     4621 gtttactttt ctctagtgca gctaacttat ctttttgctg tttattattg taatttgcct
     4681 ccaacatacc tatttctttt ttctggattt ttgtgtaaat ttccttatac tcgctttcac
     4741 ttaacccctc ttttgctctt tgaatatcgt cagctttttc tgcatagtta tcatcatcta
     4801 tagacttaac tataatctcc ttaatttggt caaatttagt tcttctttct tcatcaacac
     4861 ctgccaaatt ataagtcatt ttatctaaag cattttttct atcttcactt agctgttctc
     4921 caagtacaaa tctcatacca tcagaatcca atttaaaatc tgaaaaatca accatcctgt
     4981 tagtccattg gtattcctct tctttagctg aaagattttc cttcaaagat tttgcactgt
     5041 caaatgcccc tagacaagct tgaccaacac cactaaatgc agtcagtaaa ctcaatgcgc
     5101 tttgtattcc agaaagaatt aactgtgttt tcatttgttc ctcaagtttt gcctgtttct
     5161 tttttagttc ctctatgtct tcttgctcct cttctttcat ctttataatt tcttgaatca
     5221 acaggttcat ctttgcatta acagaaagca ttttattctc aagttcatct tttaaaactt
     5281 ctatttgttg atttccagat tcaatttgag ttaggatact ttccttataa gtattgaaat
     5341 atccttcttt taactcatct ttctttcctt gttccaattc tcttaaggtc ttgtccagat
     5401 aatctgtcaa agaacgaata tctgtaacac gatttcctcc aatttcactt tcttcaatca
     5461 tctgcacata gaatgctgtt ttttgttcaa gtgcaaataa aatcatactc cattcgctaa
     5521 aattcttctt ttctcctata aaagattcta ttctatcaga ctgcatcaat ttatttataa
     5581 ggtattcata gatttccttt gcctccttat aaaattcttt ctttatattc atatcatcca
     5641 gagaaagcac tgctttattt ttcttctcaa aactatagta cttctgaagt atttctctat
     5701 ttaattgcat ctctattttc tcaactgcat gaaaaatttc ataaatatgt gaaagttcta
     5761 aaatatcagc ttctaaaatt cccactgcac acaacagtct aagatttcta taacctttaa
     5821 ttccagaaaa accaaatctc tcgaaaaatt catagaagaa ggtatctatt gacaaagaat
     5881 actcctgctt taacaattcc ttttctttta atgcatctcc agagcgaccg tcttttccct
     5941 ctgctccgac taaacctgaa tcttcacgtt tgctaattgt ccagtcctta tgtttttcat
     6001 aaagagcatt taatttctct ccatcatctg ctacttggtt ctcatttgaa gcatgattaa
     6061 acacatatct atcaaaataa tagtaatttc tatcactgtc atctattgga gcagaggtac
     6121 ctatatatct tattgtttct tcatatatac ctctaattcc accaagacct cctgcaccac
     6181 cagctcctcc agagcctcct tttcctgcta gaccatgatt tccatctttt ttacttatag
     6241 caccttttaa taaagaagct tttccaagga gataaacctg tccagacttg ctcccaagac
     6301 ctgcatttcc accattacct cctgtgcctc cagttccacc ttgttttcct ttttctgctt
     6361 ttaccacata gtattttgta cctttaacct tagcaaattc ttttgactct tttttagaac
     6421 ctattttagc attttcccca gcttctccag ttatccctgt atctcctctt tttccatctt
     6481 gtccatttcc accacgtcca ccatctacgt aaagtttaag ttctcctctt ttttctattt
     6541 ctttaaaaga taataaaata tcacctgaag tacctgcatt attgccattt cttccattca
     6601 ttccgtcgta aatagaacct tctccaacca atttttctgc atccttacca gatacgttga
     6661 ttgaataaat cccttctggt atacataatt tttctgtaag tagtacaata ttcttacctg
     6721 agaagacttc tttctccata ttacagtcaa ttttcaactc acaatctcca tacatatata
     6781 tagatgacaa aatattaagt tgataaccag atattatttt agtcaaatcc gataaattca
     6841 ctttaggtat aggaatatat atagaaagta cattttcttt ctcatatgat tttatatatg
     6901 tacaactttg aatcttctct atcctatact ctgcatcttc aagctttttg gcttcttctt
     6961 ccctatattc ttcttctgct atccaatatt tgatatcttt taatgtatct tctaaagtat
     7021 tttttagcaa tgattttaac aactcactta gaccaacaat atgagtctca ctacagtagc
     7081 tatttaactc taaaagaaga cgtgtttctt ctaatatatt cactttctgt acgccatcac
     7141 catatgaaag ttcttgtaca aactccataa gctctttcat attattagtc ctactacata
     7201 aatctgtata catcttatct aattgagtct ttttatcctt tagactattt atagaagata
     7261 gaatggaaat agaatcttcc ataccttttt tgacactttc tatataagga actaaaagtt
     7321 tttctaggtc tttataggtt acatcaacgg ctttttgtat ttctatttta caagaagcag
     7381 taagcatcaa tcccaaatct gttttttcaa atgtttcagg tagatttttc atttcatcca
     7441 acaacttttc tggctgtcta tctagtggat tacataattt aatagataca ccatctgata
     7501 atgctacatt taataagtac actaattcct tacaaccgtc gtaatctttc tcttgactat
     7561 ctaggtcttc ttttgctttt tccaatatat tccttatttt ttcttctgtt acattaccga
     7621 ttagtgtttt aggtatttta ttgataagaa agaagaaatt ttctttatac tttgcataat
     7681 caccaagcat atctttcaaa tttttgcctg tttctatcaa taatgtatgt ctagagtcca
     7741 gtaaggattg atagtctaaa ataactacca tccctttcac ctttgaagat gtagaaatag
     7801 tttcccttac aagcatagca ccagcaatca tatactctgc tccacggtta tcaaaaaaac
     7861 ctgggcaatc acataatgaa aaatttctat ttgcaatatc tacaacttct ggacataaag
     7921 tcttagaata tacaccattt ccaatttcga cagctgaatc ttttgcagtt atatatattt
     7981 gtccagtctc ttcatccgtt ttttcttcca tcacgcaacc ttttaaataa tttatacaag
     8041 tactctttcc aacgcctgaa gcacctaaga ataacaaaat tgacttttct tttaagtcat
     8101 attttctaag ctcgtctctt ccttcttcca aaaggtttac gatttcacta taaagtttat
     8161 ttgccataca tccatccctt tctatatttc ggaacaatag ccttgatatt tttctttaat
     8221 aagattctct tctacctttg aaacagctgt tgataactta gtatttggag cattaaatcc
     8281 tccgtcgtct cctctatctc catattcagc gtattctctt gctggtcctg ttttgccttc
     8341 attccaactt ccgccactaa attctatatg atatttaatt atactatcta acttataatc
     8401 ccaacttgta aaatatcctg tatagtaaac agtcttatct ataccacttg gagaaaatga
     8461 aagtgtatta tatccgtgat agccaccact tcctccatat ccacctgaac ctggtctacc
     8521 atttggacct gcttctccat ctttcactgt tatatcacaa tgattaatta ttctattttt
     8581 ttgttcctct gagtctgaaa tgaagtaaat attaccttta ttaccagcat ttccacctct
     8641 tccggcattt ccaccagctc cacctttgtc acctttttta ccacttgtac cttttgcaaa
     8701 atacccttta aacatctgtc catggaaact acttgtatat cctgcatcag ctccattact
     8761 tccagaatct cctgtttcac catttttacc atctattcct ttagaaccat ttcctccatt
     8821 tgcaatcagt gttaaacgat aatttctaat ttcttcttta atatataagt aaatattacc
     8881 tgcatctcta ccatcttcac catttttccc atacatagaa ctatctacaa gttctccgct
     8941 ctttccactt acattaattt ttacattact acctacatca agattgttac aagcaagaat
     9001 aatatttttc tctgatgcaa agtccattcc aaggttatca tccatataaa tagtatcttt
     9061 ggcaaagaac ataaatgtag aaacattgtc atctactttt ctatttttga aatattccaa
     9121 tacactacta attactaagt ttggaattaa tgctcttatt tcaatacctt tcttatcatt
     9181 aatattaatt gtttttacta taaatggatt taaaatgtat ttaccaataa attctctaat
     9241 tatctcatta ccttcttctc catatgcact taatcttgtt atactgtctt ctaactcttc
     9301 attttgtgta actgtaacat ttgtcaaaat tgaattaaaa tcacttacac tatatacata
     9361 atccttttta tttgcaactt ccattaattt ttctctattt ggtaatgtaa atacctgttt
     9421 ttgtacttca taagaaacta aaactttttc taaattcttt cttattataa tgaagttttg
     9481 ttccattatc ttcttacttg ccttttctat aataaattcc atatgtacag aaggtaataa
     9541 aatttcccca gttccatgtt ctgtatattt acttaaagca tcaattatat ctaagtttcc
     9601 ttttatctta ttttgtaatt ccatgcaact atcttgtaag aataagccac tttgtttggc
     9661 taatacgttg ataaatctct ctatagtaag agttgttgta ctttccaacc agtttttaaa
     9721 tacttttaca acctcatcat attttgctat ttcttgaatc attgttatac ttccaataat
     9781 actgataaaa tcttctgtat acgccttaac ataatcttct aggctagata cgatattgtt
     9841 ttgaatttct tttatcaacg tatctaaaac taatcttgca tctgcactaa ttggatattg
     9901 gaatgctgat gtcttatctt taacttcatc taattctagt acttgcttta ataagccttc
     9961 cctctcgtct gattcaagtg gcttacaaag atgaatattt ttaccattat cttttagcaa
    10021 ttcttcaaat aaccaagctt tttcctctat attactatct aaattttcac ataattgttc
    10081 atattttctt tttataacat tatatatttg tttttctgtt cttcctaaga aactttttga
    10141 aatgaaaaaa gttatactgc ccctaaaatt gtctttatga agacacatat ttaactgttg
    10201 aaacaggtct agtacttttg taagtctaga atctaatata tctgcttctg gtctgaacct
    10261 cccatgacta aagtcacagg gttcctgctt cacagaattt tgcatactaa aatatgtctt
    10321 actaattctc cacaggccat ccccgtagtt cctacggttc ttacataagt tatttatttt
    10381 gaattgttat tagtcttaat ccttctttta atatatttat acttgcattt atatctctat
    10441 cgtgagtttc attacagcta ggacaaatcc attctcttat acttaagtcc ttcacttcct
    10501 cgtttttata cccacattta ttgcatattt gtgaacttgc aaagaattta cctaccttta
    10561 caatttgtct accatgccaa ttagccttgt attttaattg acgaataaat tctgaccatg
    10621 atacatcaga aattaagcga gatagtttac gatttttaat catattcttt acttgtaaat
    10681 cttctatgca aataatatca ttctctttta ttaatttagt agatagtttt tgtagaaaat
    10741 catttctttg attggctata tgttcttgaa gtcttgcaac ttttaatctt gctttatttc
    10801 tatttaaact accgattgtt tttctcgata attctctttg taatttagca agtttgttta
    10861 atgacttctt aagatactta ggattttcta t
//
LOCUS       DAMKPG010000111        10808 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMKPG010000111 DAMKPG010000000
VERSION     DAMKPG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.116"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            72..836
                     /locus_tag="PC621_003132"
     CDS             72..836
                     /locus_tag="PC621_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF4110269.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     misc_binding    1059..1272
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1526..2560
                     /locus_tag="PC621_003133"
     CDS             1526..2560
                     /locus_tag="PC621_003133"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF4110270.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2602..3819
                     /gene="argJ"
                     /locus_tag="PC621_003134"
     CDS             2602..3819
                     /gene="argJ"
                     /locus_tag="PC621_003134"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF4110271.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3834..4694
                     /gene="argB"
                     /locus_tag="PC621_003135"
     CDS             3834..4694
                     /gene="argB"
                     /locus_tag="PC621_003135"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF4110272.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4724..5911
                     /locus_tag="PC621_003136"
     CDS             4724..5911
                     /locus_tag="PC621_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF4110273.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6020..7042
                     /gene="argF"
                     /locus_tag="PC621_003137"
     CDS             6020..7042
                     /gene="argF"
                     /locus_tag="PC621_003137"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF4110274.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7169..7786
                     /locus_tag="PC621_003138"
     CDS             7169..7786
                     /locus_tag="PC621_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF4110275.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8006..8704)
                     /locus_tag="PC621_003139"
     CDS             complement(8006..8704)
                     /locus_tag="PC621_003139"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF4110276.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8866..10083)
                     /locus_tag="PC621_003140"
     CDS             complement(8866..10083)
                     /locus_tag="PC621_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF4110277.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10274..10687)
                     /locus_tag="PC621_003141"
     CDS             complement(10274..10687)
                     /locus_tag="PC621_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110278.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         4233 a         1190 c         1802 g         3583 t
ORIGIN      
        1 ttcaaaatat taagataaaa atatatagaa tatatttaaa aattattatg taattagaaa
       61 aggagataag tatgcagatt atagatataa atataaataa tattgacagt agacatatat
      121 gctgttcaat ttctgaaaaa aaaggagaaa attgtgtttt ttctaaaaaa gcttggatga
      181 aaaataattt tgataaaggt ctagccttta aacgatttga tgtaagaggt aaagctttta
      241 tagaatatat tccagcagaa aatgcatggt gtcctattac tgcggacaat tatatattta
      301 ttaactgttt ttggatatct ggaaaattta agggtcaagg atattctaat ctattgctac
      361 aagagtgtgt tgatgatgca aaatcaaaag gtaaatatgg attagttgtg ctttcgtcta
      421 gtaaaaaaat gcctttttta tctgatgcta aatatctaaa atataagggc ttttgtttag
      481 cagatacatc agaaccaaat tatgagctat tatattttcc ctttactgat aatgctccta
      541 ttccatgttt taagaattgt gtaaaaaaaa gaaaattaaa tgagaacggt atagttttat
      601 attacagtaa tcagtgtcca catacagaaa aatatgctcc tattttgaaa aaaattgcaa
      661 attctagagg tattgatgta aatcttataa aattcgaaac agcaaaagaa gcacaaaatg
      721 ctcctgcacc atttactaca tacagtcttt tttttaatgg tgaatttgta accaacgaga
      781 tactttctga gaaaaagatt gagaaatttt tagataccaa cattaaaaaa atttaactaa
      841 aaatagatgt aaatattgta ttttgagatt acattatttt ataatccaat atattttgga
      901 aatttcttaa gttgatgaat gataatataa agattataac tcaatttaga tacaaaatgc
      961 tggaaattaa ccatatagaa aatatttcca aaaatttaaa aattatattg acaaagttaa
     1021 aaaaataaac tatcatataa aacaataaga aaataaattg aaatcctgtg aaaaagagag
     1081 tagcataaag ctagttgata gagagctggg aatggtggaa gcctagtaac gaattttatt
     1141 gtgaatagag ctttggagga gattaccaga agtaatagta tggtaattgg tgaatctcgc
     1201 cttaagagat tagagtgggt aagattatct tatcaattag agtggtaacg cgggtgtata
     1261 ttcgtctctt ggtctttgac caagaggctt ttttaatttt aaatgtacat tttgtagata
     1321 ctttttaatt tgagttttaa tgattattgt aattttacca atgagagtgg taccgcggaa
     1381 aatcgcctct tagtatttat aatgctaagg ggttttttgt tttaattaga atattttaat
     1441 ctacatatta tcaaatgatt atcatatagt aaagtggaaa gatagaattt taatactatt
     1501 tgaatataaa aatggaggga acaaaatgaa taaaataaaa gttggtatta ttggcgcaac
     1561 tggatatgtc ggggcagagt tgattagatt attaatgaat catgataaag tcgaagtgac
     1621 agcaataggt tcaaattcgt atgttggaaa aaatatagtt gatatatatc caagtatagg
     1681 atataaaaat aacatgatat gtatagaaaa tgaaaaagtt atagatatgt gtgatgttgt
     1741 gtttacagcc cttccacatg gagttagtga aaagtttgta ataaaagcaa ttaaatcgaa
     1801 aaagaaagtt atagatttag gtgctgactt tagaataaaa gatgaagaag tatatagtaa
     1861 atggtatgga gtatctttta tagataaaat cttacataaa aaagctgtat atggattaag
     1921 tgaaatatac aaagaagata taaaagatgc agatataatt gcaaatcctg gatgttatcc
     1981 aactagtatt tctttaccat tgatgccatt actaagttct aaattgataa aaaataataa
     2041 tataataatt gattctaaat caggtcttac aggtgctggt agagaattat cgattagtag
     2101 tcactttacg gaggttaatg aaaatatcac tgcatataaa atagggaaac acagacatac
     2161 tccagaaata gaacaaaatt taagtgaatc ctgcaaagaa aaagtaagtg tagtttttac
     2221 accaaactta ataccagtaa ataggggaat cttatcaact atatattgta caaaagaaga
     2281 taatatttct ataaatgata tacatagaaa attaactgat tattatgaat ttaaagaatt
     2341 tatagaagtt ctcccattag ataaggtagc ttctttaaag aatgttaggt tctcaaataa
     2401 atgtgtcata tcattacatg aaaatggaga tacattgata atctgttcag ctatagataa
     2461 tatgattaag ggagcagcag gtcaagctat acaaaacatg aatataatat ttggtattga
     2521 agaaaataca ggtttaaaaa atattgcacc atcattttag taatgaaaaa atactaaact
     2581 catataatta ggaggataaa tatggagatt ataaagggtg gagttacagt ttcagaagga
     2641 ttttttgctt cagggattca ctgtggcttg agaaaaaata aagaaaaaag agatttagct
     2701 ttggtttatt cagatgtttt atgtgatgca gcagcagtag taactcaaaa taaagtaaag
     2761 ggcaatcctg tttatgttac acaggaacac attaaaaatg gaaaagcaca agctatcata
     2821 gtaaatagtg ctaatgctaa tacctttaat ggaaaagaag gacttataaa tgcttataaa
     2881 atggctaaat tcactagtga taaattaaat attaaagaaa atgatgtttt ggttgcatct
     2941 acaggtgtca taggaaagcc attaaatata aatttaatag aagaaaatat ggatgaatta
     3001 gtgaataatc taagtaaaca aggtcatatt ggagcaagag aagctattat gacaactgat
     3061 attataaaaa aagaaatagc agtagcaata atgtatggag ataaaaaaat aactattgga
     3121 ggtatggcaa aaggttctgg catgatacat cctaatatgg caacaacgtt aggatttata
     3181 actacagatg caaatattga tggagttttg ctaaaagaag ctttgaaaat tgctgttgat
     3241 aaaagtttta atagagttag tgtagatgga gatacaagta ctaatgatat ggtgatgatt
     3301 ctagcaaatg gaaaagcaaa aaatgataga ataaacaaaa aagatgaaca ttatcaagta
     3361 tttttgagtg cacttacata tgtctgtata gaacttgcta aacttgttgc aaaagatgga
     3421 gaaggatgta caaaactaat tgaatgttat ataaatggag cattaagtga agaacatgca
     3481 gtaaaattag caaaaacagt tatatcttct tcattagtaa agacagctgt atttggagct
     3541 gacgcaaatt ggggtagaat actgtgtgct ttaggttatg caggtgaaga aatagatatg
     3601 gaaaaagttg atattatttt tgagagtatg aaaggatata ttgaagtatg taaaaatgga
     3661 aatggattag attttaatga ggaaaaagca aaaaaaatcc ttgaagatga cgaaataagt
     3721 atattagtag atttaaatat gggcaatgcc agaggaaatg catggggatg tgatttaagc
     3781 tatgattatg taagaataaa tggaagttat agaaattagg aggatatact ttaatgatta
     3841 atatagaaaa agcaaataca cttatagaag ctttaccata tatagaaaaa catcagggaa
     3901 agactattgt agttaagtat ggtggaagtg caatgaaaaa agatggtcta aaagaatctg
     3961 ttatggaaga ccttgtatta atgagttatg ttggaatcaa tatagtttta gtacatggtg
     4021 gaggggcaga aatcaataag atgctagcaa aagtggatat agaaagtaaa tttgtaaatg
     4081 gtcttagata tacagatgaa gagaccatgg aaattgtaaa aatggtactt gctggaaagg
     4141 ttaataaaga cctagtaaat aaaatacata caaaaggtgg aaaagcagta gggctttgtg
     4201 gaatggataa taatatgatt ttatgtgacc catataaaaa ttatgaactt ggatttgtag
     4261 gagaaatcaa aaaagtaaat gttgagctta tagaatcttg tctaaagtca ggttatattt
     4321 cagtaattgc aactataggt gttggagatg atggagagac ttataatata aatggtgaca
     4381 cagcagcttc tgctatagct aaagagttaa atgcagataa attgatactt cttacagatg
     4441 tccctggttt gttaagagag ccagatgaag aaaaatcatt aataacagaa gttatattag
     4501 aggatgttga taaattattt gaagaaggta taattactgg aggtatgata cctaaaatag
     4561 agggatgcgt agatgcctta aataacggag taaatagggt acatattttg gatggaagag
     4621 ttccacattc tataataaca gagctattta ctgatagtgg aataggtaca ttaataagaa
     4681 aagaaaatga ataaaattat aagtttaaga ggggagaata agaatgaata acaacaatac
     4741 aatttcaaaa tggaatgaat attttataga tacatataac caacccaatt ttgtcataga
     4801 ttatggtgaa ggaagttgtt tttttgatac taatggcaat aaatacatag attttacaag
     4861 tggatatgga gtatcttctc ttgggtatag taatagtaat ttaaagaatg ctctaaaaga
     4921 gcaagtagat aaattattac atacttcaaa tttatatttt aatgagccag tgttgtattc
     4981 aggagagaaa ataattaatt caagtggaat ggctaaagta tatttttgta attctggaac
     5041 agaagcaaat gaaacagcat tcaaaatagc aagaaagtat agtagtgaca aatatggtaa
     5101 tggtagagga actatcataa gtttaaaaga ctcttttcat ggtagaacaa tgatgtcttt
     5161 aatggcaact ggtatggaca aataccataa gtatttttat cctcttcccg aaggttttaa
     5221 atatatagaa agaaataata ttgaagattt aaaaaataat cttgattcca cagtttgtgc
     5281 aataattctt gaagcgattc aaggcgaagg tggagttaat gttcttgaaa aagattatgt
     5341 tttggaaata gtaaaaatat gtcaagaaaa ggatattgta gtgatttttg atgaagtcca
     5401 atgcggtata ggaagaactg gtaaactttt tggctatgaa tattttgatg taaaaccaga
     5461 tatagttacc gtagcaaaag ggttgggagc aggaatacca gtaggaggag tattggtaaa
     5521 taaaaaactt tcaaaagtac taggaaaagg agaccaaggt acaacttttg gaggtaattt
     5581 attagctatg gtggcagctt cagttgtatt agatgagata tctaaagatg gtttttataa
     5641 tgaagtatta gaaaaaggaa attatataag aaaatctatt gaaagtttta ataataaagt
     5701 tgtattaaag acaaaaggta ttggtctaat gataggaatt gaaactaata ttgaatctag
     5761 tattatagaa gagaaagcta gaaaaaaagg attgcttatt ttgactgctg gaaaaaatgt
     5821 attgagattt ttaccaccat taacaatcag ttataaggaa atagatgagg ctcttgaaat
     5881 cctaaaagat atattactag agattaacta aatattcaaa aaatagttaa ttaaagataa
     5941 aaattagaag taaataaaaa agtaaagtgc taacaagttt aattgaattt aagaagttta
     6001 taagaagggg cgaattaata tgaataatat aagtttaaaa ggtaagagtt ttttgacatt
     6061 aaaggatttt acaaaagaag agattagata tttattaact ttatcaagtt atttaaaaac
     6121 taagaaaaaa gttggcataa aaggcgattt gctacaagga aaaaatatag ctttattatt
     6181 tgagaaaaca tcaacaagaa ctagatgttc atttgaagta gcagctcatg atgaaggagc
     6241 atatgtaact tatctaggtc caaatgattg tcatatgggt aaaaaagagt ctgtatcaga
     6301 tacagcaaaa gttctaggaa gattctatga tggaatagaa tttagaggct ttaaacaaga
     6361 aacagttgaa agtctagcaa aatattctgg tgtacctgtt tggaatggac ttacagatga
     6421 atatcatcca acacaaatac tggcagattt tttaacagta attgaaaatg tagataaaga
     6481 tttgaataaa gttaaatttg tatatgttgg tgatgctaga aataatatgg gaaactcact
     6541 gatgatagga tgtgctaaaa tgggaataca tttcgtagca ctagcaccta aggaactttg
     6601 gccaaatgaa gaattggtta aggaaatgaa ggaaatagcc aagaaatcca aaggagatat
     6661 aatgttgact gaaaatattg atgatgttaa aggtgcagat gttatatata cagatgtatg
     6721 ggtttctatg ggagaagagg agcaatatga agcaagaata aatcaactta aagattatca
     6781 agtaaatatg gatatgataa agaaaactga aaatgaaaat gttatatttt tacactgtct
     6841 tccagcattt catgatttag aaactaaggt tgctcagaag atttatgaaa agttcggatt
     6901 atcagaatta gaggttacaa acgaggtatt taattcaaaa tattcaaagg tatttgaaga
     6961 agctgaaaat agaatgcata caataaaagc tgttatggtt tctactatga ctgatgtaaa
     7021 tatgaacaat aatttaatat agatatgttt taatatagac tttactttaa tgaaaattta
     7081 taaaatattt taaatatatt attcaaaaac tctcattata tgttaaaata acttatgtat
     7141 caacaaatta agagggagga tttgagatat gaaaaaaatc atattagctt taacaacttt
     7201 tttattagca atatctttaa cagcatgttc aagtgcaaaa ccagaagata caattgatag
     7261 tttttttaac agtgcgaaaa agtttgactt tgaaggcatg aataaggtaa tggaaaataa
     7321 tgacgaaaaa tataaggata tattaaagga attagataca aaagacccaa atgcccaata
     7381 tgttttagat tatctaaaac agaatgcatc taaaatcacg tatacaataa aagacagcga
     7441 ggttaaaggt gataaggcta caatcaaagt agaatgtaaa tttatagatt caacaccatt
     7501 acttcaagaa atagtagctg aggcatttac aaagatgatt ggtatgagtt tttcaggaca
     7561 agacttaact gatgagaaaa caactgaaat gttagtatca attatgaaag aaaaacaaaa
     7621 aagcgttaaa gagacttatg ttacaaaaac tgtagagttt gaatgttcta aaaaggataa
     7681 taaatggata ataagttcag taaatgatgc agtagctgat gtattgttat ctaatcttgt
     7741 tacagcagga caagaatttt ctaactctat gaatacactt gaataatatt gtttaggtgt
     7801 agtaaagtaa taaatatatg attattttta taaataaaca tataaatatt tatgaaggat
     7861 accatttttg gtatcctttt tattaatata atgaaataca tggttaattt ctatattgtc
     7921 aagaaactat gatatatgtt ataagtctat actatttaaa ataaacttat aacatatatt
     7981 ttttataata tagtgcatga aaaatttatt taactaattt tagatattta ttgacaactg
     8041 attcagccaa gcattttgat gaatcaacaa tatttattga aggatatgat ttttctaaaa
     8101 ctacatttaa atcagtacaa gcaattacta tattttctat agattcattt ttaacatctt
     8161 cgataagttt attccaaata tcaatattgt gtggattttc tttatccatt tttatatttt
     8221 gaataagatt gtttaatttt atttgccatt catctttaaa tataaattca tgtccattat
     8281 gtattatacc attctggtaa attgtagact caaaagttga actagtcgaa aaaagagtta
     8341 ctttttgtga gatagtaggt aattttttaa ctgtttcttc tacaatattt aatattggta
     8401 tattaataga ctcttttaat tctttaaagt atatatgtgc tgaattgcat ggcattgcaa
     8461 taaaatttac accagttgat tcaagctttt gtaatccatc aattatagtt tctttcataa
     8521 gttcatgatt tattggacgg tcaatataaa atggggtagg taatgaatat atcatcatct
     8581 ttggaaattc atcatcatat tttgctccat attgagattg acactcatca ataattaaat
     8641 caacaaaagg ggctgttgac cttggaccca ttccagccaa aataccaatt aatttttcat
     8701 tcataaaaat tatctccttt atatataaat atatttatgt ataaaataaa aatctataaa
     8761 agcataaaat tatattttat aatagattat attgtacact tgtataatat aaatataaag
     8821 tgatttttta gattatatat tttaatatac tttcttatat aaaaattatt ttaaatcaag
     8881 attatctagt atttttaaaa atatttttac agcagtatcc aagtcatcta cagaaacact
     8941 ttcattagga ttatgactta cgcccccagc acatcttata aataccatac caatatcagt
     9001 tatattatcc atttcctgag catcatggcc agcaccacta taaatataaa aaggattaag
     9061 atttaaatct ataaaacttt tttctatgat ttttgttatt ttattagaac atggtacagg
     9121 aacattttca aaagcaagtt cacttgtata attaagtttt ctattttcac atatatgtga
     9181 tatttcatta aatatctttt ctacagaatt atcaagaatt tcttgtgaac aagagcgtac
     9241 atcgagtgta aattcagctc tttcaggtac cacatttgat gaagatggat agaaattcat
     9301 ctttcctaca gtagcaacca aatcagcagt cgttttagct actttttcga tagctaatac
     9361 acattcacag gcacaacatc cagcatcaag tctcatattc attggaagtg taccagcatg
     9421 tccagactta ccatttactg atatcttaaa acttttaaat cctgtaatac ttgatacaat
     9481 tccagcagga agagattcat tttctaaaac aggaccctgc tcgatatgaa attctaaata
     9541 tgcatctaca tcttttctag gatgcaaatt tgttaagttt tttgtattaa atccaaactt
     9601 tgttacagct tcttcaagtg taattccatt ttcatcaaca gatttaagaa gttcctcttt
     9661 aaaagtacca gcaaaaactt tacttcccat taaagaagtt tcaaatctag ttccttcctc
     9721 ttcagcgaaa gcaattagct taatattgaa aggataagac ctattattgt gttttgcttc
     9781 ttcaagacca actagaggca ctattatacc taacatacca tcaaatatac ctccacactt
     9841 tacactatct tgatgagatg caactactaa tgtgggagca tttggaaagc ttgatttata
     9901 tgtacctata acgtttccaa tgtcatcaat ttcaatatca agatttaaac cttccatcca
     9961 tgaactaatt aaatcaatag attttttatg ttcttgagtc aagataagtc ttgtaagacc
    10021 atttgttgtt gaacttattt ttcctaatgt ttgtagataa tccatacatt tttttcctaa
    10081 catatttacc tcctaaaaat ataattttta tttaagctaa gattagcttt tatacttata
    10141 atttttgaac tttcaaataa ttttgtatgt atagtaaaat actattttag tattaataat
    10201 gatataaaaa cataaagtct gtagacgaag agcagaagtt ttaagttaaa aatataaaat
    10261 atgattttta ttattatagt atttttctat aataatatct ctttccagtt agaggataat
    10321 tttcaagtga aaatacttta tggtatccaa atttctcata aaatttaggt gcttgaaaac
    10381 tgaatgtatc taaaaaaaca tatttacaac ctcgttcttt agcaattttt tcagtcttat
    10441 ataaaagttg acttccagta cctttatatc taagcttctc actaacccat aagtatttta
    10501 tagaaagcca attaccatga gtaacaccta taaaacctgc aacaatttta cctgctttat
    10561 ctcgaatata tattcctaaa tccactgggt ttttatcttc aagtctagca agattatatt
    10621 ttaataatcc ttgaaaaata gtatcttcat cttgtttttc tattttttct gtaatttcta
    10681 attccatatt tgaactttct ccttgtataa tcttttgtat attggtattt tttaataaaa
    10741 tcagtagaaa gaatatagtt taacattatt ctaataggca tttatgctaa tgtcaaacta
    10801 ataatact
//
LOCUS       DAMKPG010000112        10681 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMKPG010000112 DAMKPG010000000
VERSION     DAMKPG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.117"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(18..662)
                     /locus_tag="PC621_003142"
     CDS             complement(18..662)
                     /locus_tag="PC621_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110279.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            847..1470
                     /gene="ytaF"
                     /locus_tag="PC621_003143"
     CDS             847..1470
                     /gene="ytaF"
                     /locus_tag="PC621_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF4110280.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            complement(1533..3443)
                     /locus_tag="PC621_003144"
     CDS             complement(1533..3443)
                     /locus_tag="PC621_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF4110281.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(3980..5047)
                     /locus_tag="PC621_003145"
     CDS             complement(3980..5047)
                     /locus_tag="PC621_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF4110282.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            complement(5293..6288)
                     /locus_tag="PC621_003146"
     CDS             complement(5293..6288)
                     /locus_tag="PC621_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF4110283.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            complement(6547..7806)
                     /locus_tag="PC621_003147"
     CDS             complement(6547..7806)
                     /locus_tag="PC621_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF4110284.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            complement(8045..8674)
                     /locus_tag="PC621_003148"
     CDS             complement(8045..8674)
                     /locus_tag="PC621_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF4110285.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(8867..9028)
                     /locus_tag="PC621_003149"
     CDS             complement(8867..9028)
                     /locus_tag="PC621_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110286.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(9371..10531)
                     /locus_tag="PC621_003150"
     CDS             complement(9371..10531)
                     /locus_tag="PC621_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF4110287.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT         3503 a         1750 c         1053 g         4375 t
ORIGIN      
        1 aaatattatt ctaaaactta cttatcattt ttaagtaatt ttgtaactat atttaatgct
       61 actaaatcat ccataaatcc tattgggaac acgaagtctg gaatcacatc tgttgatata
      121 acaaagtaaa gtagcatact tccaatcaat aaatatttct ttattgtaag tccatctgta
      181 caaaaatcag catacatttg tcttaatcta tctgtaaatt gaccttgttt ttcaatagaa
      241 tctatttttt tattaaaatt atcaaaaagt aattttttac cttcatctgt ttggacatat
      301 aacttacact tacttaactc tttttcaata tttccattaa agttcttatc attgattaaa
      361 tttgcaaatc caagaatatc atcaaagtta ctaaaatcac tatctgcatt aaatatttga
      421 ttattgctgt ttctaaattc atatcctgaa gctttcaaca gttcttctag gttaatattt
      481 aaatgtattg ccatcttttc taaatgattt atatttggcc tttgtttatt attagctata
      541 cgagatatag tagatttatc aattccactt tttttactta gttctcccat tgataattct
      601 ttttcatcta ataaaacttt caacaaatca ccgaaattcc caaccttact atgttgttcc
      661 attattttca cctgctttat tatgattggt tttatattat atgatatgat tcttttgtta
      721 tttaggtgtt gtatgatgtt gaaattttat caacaattta ttggattatc tgtaacaatt
      781 tatccatttt tacaatacta taactaatat aagtacaaaa tttaagagaa aggatgttaa
      841 tctactgtga ttgaatcaat tttattagtt acttctcttt gtatagatgc ctttgtgaca
      901 agctttggat atggagtaaa taaagttaaa gttcccttat attcaaatat aattatcagc
      961 gtagtttgtt cagctatttt agcaatatcg ctttttgcag gtacaatagt tagagatttt
     1021 ttacctcaaa atataacaat gttaatatgt ttctttattt tatttttatt aggaagtgct
     1081 agactaattg aaagtttttt taaatcttat tttaagaaaa aatctataca ctcaaagaat
     1141 tataatttta atatgttaga ccttgaatta aattttaatg taagtatcga acctgatgtc
     1201 ttagatacaa gtaaatctaa agtgcttaaa tctgctgaag catttacact tgcacttgct
     1261 ttaagtttag atggattagc tatcggtttt ggtagtggcc ttgtaggtgt gaattatata
     1321 caagttattc tattttcttt aatatcaaat ataattgcag tattacttgg ttgtttacta
     1381 ggaaatatgt ttgttaaaaa cgtaaattta aacttatctt ggttaagtgg tttaatcttg
     1441 ttagtcattg gatttatgaa gatatgttaa taagaaagca atctaaagat aaacttttct
     1501 ctttagattg ctttcttttg tgctttttat atttattttc tattctctct aatttctttc
     1561 tgaatctttt taagagcttt ttttgaacct ctttgaatat ctttttctaa ttttctttct
     1621 ttaaaactag taaataatac atctaaatca tacccttctg gatacaattc tgatgctttt
     1681 atgctaagtt gtaatctttt tatatttact gaaataaaat tatcattaaa caatacttct
     1741 acattattaa acttgtccat aggcttatat acaattccaa aatcatcttc atctagtaat
     1801 tttaccttat cccctacatt ataatctgta tagcttaaaa tgacttcaga actattttta
     1861 tcttcaatat tagattgtag cttacttctt ttaactaaat ctaaattgta gtttctatca
     1921 attacatatt ctttagctct atctaaaact ctatttttta ttcccatttt tttagaaata
     1981 aaaagagcat tactatcttc agattttcca atagttaact tatacaatgg ttctaacgtt
     2041 tctttatcaa acatcatacc agcattttca aattctggat gtaatgtagc aaactttttt
     2101 atctctccat aatgagttga tgctatagtt atacatccca ttttgtaaaa ctcttctaat
     2161 aatgatattg ctaaaccagc tccttcatta ggttcagttc cacttcctat ttcatcaaac
     2221 agaacaagag tagaattatt tgataaactc ataatctctg caatattttt tatatgtgat
     2281 gaaaaagtac ttaaggcatt ttcaatactt tgattatccc ctatatctac aaaaactttt
     2341 tcaaatacac taaattcact accttctttt gcagaaatat caaatccaca ttgaaccatt
     2401 aaagttagta aaccaacagt cttaagggtt acagtttttc ctcctgcatt aggacctgtt
     2461 attataagac ttctataatt tttgcctatt tcaaaatcta aaggtactgc atctcctgtt
     2521 agaagtggat gtttaccttt aattattttt atatagccat tattatttat ttttggagta
     2581 attcctttta ttttttgact atacttagct tttgcaaata ccatatcgta ttctgaaagt
     2641 atctctatat ttaattttat ctgacttatc ttctcaaata taagttctgt taaataagat
     2701 aatattttgt attcttcaat agtttcatca gcttttagca tagttaactc tgtacttaaa
     2761 tttgaaacac taataggttc aataaataca gtatttcctt ttgatgatgt atctaatata
     2821 gtaccattaa cttcattttt ataagaagat ttaattggta ttacatatct atcatttctt
     2881 ttacttatta taaactcttg tatatacttt ttatttacag ttgatgttat aaatttattt
     2941 aatctatctt ttatctttcc ttctgtaatt tctatatttc gtcttatctt ttttaattct
     3001 ttactagcat tagaatcaac tttattagat tttatacagt aatttatctc atcttctata
     3061 cttttacatt ctgttatatt taaagcataa gaacttaatg taggtgaata aaattctttt
     3121 tctaacataa aagctttcat cttcctacaa cctcttaaaa aatcttcaat atttacaagt
     3181 tcaacaggtt ctattatcat tcctttttca attttatcta ttgtagaacc tgcattaaac
     3241 aatccttcaa gtggtatgtg attactattt tcaattatct ttctagcttc tttattttct
     3301 gctaatttcc ttcttactac tccaatattc ccactaggag ttaatttatc tattaaattt
     3361 tttcctagcg aacttacaca atgtattttt attaagtctt taacttcatt tagttgcaat
     3421 ctttcaaatg tattactatt cattattttt tctcctttat ttataagtca tcgtctttta
     3481 aatcacccta tcatttactt tgataatagt tattttgcga acaacttaat aaataaggag
     3541 ttattatata tcaaactcct tatttattaa gaagtttaac tccggactca ttaatgttag
     3601 tatttttttt cataaaacac ttctcctttt ctattcttat ttaaacactt ataccagtgc
     3661 ttctaatatt attccataaa ttatatattt atgtcaataa aaataatgtt attaatgttt
     3721 ttgtacagtg aaaattttta tagtgaaaat aagcttatac tttcatttat attttggaat
     3781 tacttatatt gtcatcaatt catgtatgtg tagtttccaa tacaatctaa ctttgtaatc
     3841 attatatatc aaagaattta gctctataaa ttatcgtctc tataaaataa aaaaatagct
     3901 aataataata aaacactttt aaatgttcca taaaattagc tatttataat ataattttaa
     3961 tcttttttaa ctttaaaaat tatcctttat aagtaatcaa tggcttcata gttgccacta
     4021 cttgaataag ttcatattct atcaaacttt caataatctg ctctatattc ttatatgcct
     4081 ctggagcttc ttgaaataat aaatttacat cattacatat aatctgactt ttaaatttag
     4141 tttgttttat agtatcttta ctatacttac ttttaaggcg cgatttacaa actgaacgag
     4201 accattttct acctgctcca tgtgatagag aatataaaga tatctctgta ttttcagtag
     4261 gcataactat atatgataga ctgcctcttg aacctggtat aatcacagct cctctttcac
     4321 ttgaaacagc accttttcta tgtataaatt tatcttccct tttctctata aagttatgat
     4381 ttatagagaa tattgtttca acatcttcag atgtacctat tgactgcata atttttttac
     4441 tgatagcttc acgattacgc tctgcccaca ttaaagcatt atcatgtttt ttaatataat
     4501 ctattgcttt ctcactttca acttcaagtc catctttatc ataaaatttt tttaaaatat
     4561 cttctccata atttcttgac ccacagtgaa ctaacatcat aatatcatca gatgaaaatt
     4621 ttaattgttc aaattgttct ttattataaa tttcacttat acattgaatt tctacaaaat
     4681 gatttccact ccctatagtt cctaaattta aaattggaca ttcttcttta tatgtgtttt
     4741 ttatttctat atcggacaaa tctttaatct tactaagtga cttaatccat ttgtcttttt
     4801 taaatttctt ttttagaacc ccagttttaa ataaagtcat tccgcatcct atatcatttc
     4861 caattatatg tggataaata atatttttag tctcaactgc taaacctaca ggtattttac
     4921 ctgtatgtaa gtcaggtaat cctactactc ttaaaattcc atttaacttt gaaatatctt
     4981 ctagttgttt ccttgctata tcttcaatcc aacatttatt acttgctatt atttttatat
     5041 tgctcataat ttgctccttt ttaatattct cctgccttta aaaagtagca aaaagctgtg
     5101 acctttagcc acagcttttt tattctaaac tgtaaaagca ggtggctatt tgttaaaata
     5161 ctcctagacg tttaatacct cattattttt tgttaacata ataaccactt cctttcataa
     5221 tttaagacta cactaaaatt tctatgtata tcatttatag acattgattt taaaatactt
     5281 aatatacaat ttttatatta tggaactaat aataatcatt gaaatcatga ctatagctag
     5341 accggcaaca acaagttttt tatgtaattt ttttctcatt ttacttttat attctttttt
     5401 aacttgtata actttgtctt gtttctgttc ttcttctttc tcatcttcaa cttctatata
     5461 tgctttttca tacattactt tacttaaatc aataataata tcattggctc tagtatactt
     5521 attatttctt ccaagaagcc tttttataac atttataagt tctgtatttc cattaattgc
     5581 ttttatacta ttccaatcta atcccttatc aatagattgt agcatttttt cttctgaatt
     5641 tgattctcca aaaggcaatt ttttaaatat agattcaaat agaataagac ctgttgcaaa
     5701 aaaatcactt tctttatcag tataatttat acatagttga tgtgggcata aatatctcct
     5761 gtttccatag cttcttgtat taaccccatt atttgctttt gtaattccaa aatcacaaat
     5821 ttttatattg tattctgtat ctactattat actactcgat tttaaactac catgatatga
     5881 atgattccta tgtatcattt ctaaagcttt taaaacttgt atcattatgt ttacaattgc
     5941 ttctaaatga agataattac ctagaattaa ttcatctagt ccgataccat taaagtcttc
     6001 acttacaaca taatataatg ttgtttcttc tgtacagtga atcccaacat ctattatttt
     6061 tagtatataa ggagagttta tctcatctaa agcagtactt tcatcaatta aatttgaaac
     6121 aaaatcttca caaatataat tattgtgctt tataactttt attaacacat ttctcctgta
     6181 aaaaacatct ctagctttat agattttgtt aatttctaaa acgtcatcag aatttactac
     6241 ctcatatcta ttgcctataa aattcaacca tgtcacctcc tcatacattt tatataacta
     6301 aatctattta caattatacc aatatatagg aatattttct atatattaat gattgtaata
     6361 ttaaaacaag aactaatagt gttaattttt acaaaaaagg ctgtctcaaa atagagatac
     6421 gcttcttttg tattgttgaa atgatgaaaa tatatttgat ttcacataaa aagagtgtct
     6481 cataaactaa aaagttcatt ttgagacact cccttttata ttacaaatat ttctctaaaa
     6541 aattatctat ttttttaatt ctttataaga actatttgat atatgttctt tttttatgac
     6601 ctttccactt gaatctttaa atattcgata tgtagaatat ttattctgac cagtttgttt
     6661 tacctctata gatatatttg gtttgtcacc agaattacca tagattactg atgatacact
     6721 tcctccacct gcataatttt taatatatac aggatgctta tatggatttt taaactttaa
     6781 gtttaatcct ccatcgctca cagtagcatc tcttccttta ggcacataag tagatgcaga
     6841 tgaatgattt gtaacttgag ttatctccat acccgaaaga agagccgcat tataaactgt
     6901 tgaagatgtt tgacaaacac ctcctcctgc tgattgttct agcttaccat aaactataac
     6961 aggtgcatct ttgtaaccat ttgctttatt actaggtcct gtaagtttat tgtatgaaaa
     7021 ttcttcacct ggcattaata atacatcact agtttttctt gctgataatc ctacattata
     7081 tgacctaccc tctacagctt gtgaataagt agttgaatat tgacctataa cagaatttac
     7141 agtttttact tgttcagttg ttatatctgg tttttgaaga tttactttaa gttctaaagt
     7201 tttatgctct ttatttttta tcatattata tatagactct ttatttgctg ccaaatcaaa
     7261 ttctctacct acaacagagt ctgtataact tagtccacca cttccaacac taatagaagc
     7321 atttttcatc ttaacattta tactatctgc tattgaagac actttggaac taagttttgc
     7381 ctcatcatat tcagctttta tcttcatatt tgccttattc actcttatat caatatattt
     7441 ttttaagttt tgaaaatagg accctttttt tgtactttca taagcatctt ttacaacatc
     7501 ttctgtatca tattttaaat caatatcttt tggtgatatt gtatattttt tcccatcata
     7561 agtaagattt aagtcttctg gagtatactt atctgttata gcttttaatg cctctgcttt
     7621 agtcatatca gatacattta ctccttctat atatacattt ttagcgattt tcccattgta
     7681 cagaaattta ctgttaaaaa aataagtaaa tccacccatt attagaacta aagctgccaa
     7741 tacaattaaa acacttcgtt ttttttcttt acctaatctt gaaactcttg ttgttcttct
     7801 gcccataact tcacctcatt ttgacactag cttaaatttg cataatagag ttcccatttc
     7861 aaaactacct tagattatat ttgaatagct tttgttaaac aaggaattta atcaaatgta
     7921 actttattgt aaatttatgt cttataatta gttttatttt tgatattttt tattatattt
     7981 cttttactat ttcttaacaa aaaattgttt attttataac aactaatttc atttatattg
     8041 aaatttaaat gtaacctatt acaggataat ggtctgacag gtaaattttt tctactgtgt
     8101 atccttttaa ttcagtattt ttattaacaa aaatgtaatc tattctagag tttgattttt
     8161 caaatgttgg taagtaagac ttgtttagtg aaatagccat atcattaaac atacttaaaa
     8221 agacattttt ctcattaaaa tcaccacata aaataactat ccccactaat ctgttcctat
     8281 agtctattat ttcgtctaat tgttttgctc tctcctgcct atcaagtccc aagtgagtat
     8341 ttattacatt tataattctt ccatatgcat ctatagttat acaaagagct ccccgttgtt
     8401 cttttttgct agttaagaaa aaatgattac tgtttaacat tttagatgtt gtaaatgtac
     8461 aaataccata tatcatattt acttttttta catttgtagc aaagacacca tccatattta
     8521 aaacagcttt taaagctaaa aattgaggat ataatacttc ttgcaaacaa attacatcac
     8581 aatccaattg ttttaaatat aaccccattt tagttagagt taatctatta tttgaatcca
     8641 tacctttatg aatattgtag gttactattt tcataataat atcacctctt atgcaatata
     8701 atacttacta aaatttaaac ttatttactt ttcacttaag accattattt atcttataaa
     8761 attgagtact tataaacttt atcacaaaaa aacctaactt aagtatttat attctacata
     8821 agttaggttt tataaactat tataatattt attatttcat tcatttctaa tgatgattct
     8881 ccttttttat ctttttcaat ttgccttcac taacaacaaa taaaaatccc atcttcttgt
     8941 atatttcatc caattcaatt aaagttactt caccattttt taaatttgtt aatgttatat
     9001 cttgtattct tcttgatgga attatcataa gttatcaaca ctcctttaaa ttctttttaa
     9061 tctttatatt taaatatatt tatgcattct gatatatatt cataaattat taaaataaat
     9121 tcaaaaaaca cccgttctgc aaacattcgt tctgtatatt tattatacat catacagacg
     9181 ttcgattcaa tataaaaatt aaatatatct cgtttttagt aaattttaca atattataca
     9241 ctaaatatgt caaaacatct tcttgaaata gattatcttg tcaaacaaaa aaaaccattt
     9301 cagtaaaagc ttctgcttaa attgaaatga cttttcttat ttaaattatt aattttatac
     9361 catattttta ttaagctaac atatgtaata tttgagttac cacaagagca caataagtcc
     9421 ctataatatt tcctagaagc cccataagta cacctactgg tattaataca ggattgtgaa
     9481 ccccagcaat cattggagct gtagttactc ctcctatatt agctatactt cctacttcac
     9541 aagtaaataa atcaagttta aatagtttag caagccctat aaatgaaata gcatgtacaa
     9601 ggactactac caatccaaac attatgtaaa caggtgcttc tgacaatcca aagaaatttg
     9661 ctttactacc tgtagctgca aggagtaaat acataaacac atttcctacc agtgtactcc
     9721 ctttaagctt tgatattttt gtcataccca ataatattga tactgtagat gcaacaagta
     9781 ctacccacat agtttcatta agtatacctg aagttggaag tatcttggat acaatttgtg
     9841 caaaagacgc aactccaaac ccaacagaaa gtaaaatcat aagttctaca aacgtaggtg
     9901 ccgtattatc tttattttct ttttcaagtt ttgatgtaac ctcatctata aaacttgtat
     9961 cgcactttgt ccatttgtta aatttaggtc caaatttcac agacattaga caaacagaca
    10021 tccatactgt atatcctata gaacccataa gtattgcata actaagtcct tcttctgcaa
    10081 ctcctagagc attagctgct gcaatcatat ttgatgagcc acctatccat gaaccagatg
    10141 ctgccccaag aattttccat gaatctgcac ccattgaatt tttaaaaatt atgaatgcta
    10201 taacaaatcc aagcataaat gtaaatgtag cacagaaaaa tgataataac attttaggcc
    10261 ccatctttaa tatttttctt aaatcacatt gaattaacaa gaacactaac attaatggaa
    10321 ggaagttcac tgttattatg ttttgatacg ttccaacatc ttcattctgt gcaaataaac
    10381 caaatgatcc aagtatagct gaacctaagt atattaaaag cattggatta ataacatcaa
    10441 ataccttcca tttcaattta tcaaccataa aaaaaattac acctacgaat acaaccaaga
    10501 aactacaata tacaaatcca gtctcaatca cattatacac accctttctt ttcctaatta
    10561 atttgatttt tatgatttct aagtaaagac tttttcagat aatagacaat taaaataaat
    10621 acttttatct atatcatgct aattaaatac atatatacct aactattgac atatattaat
    10681 a
//
LOCUS       DAMKPG010000113        10400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMKPG010000113 DAMKPG010000000
VERSION     DAMKPG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.118"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            184..963
                     /locus_tag="PC621_003151"
     CDS             184..963
                     /locus_tag="PC621_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF4110288.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1273..2094)
                     /locus_tag="PC621_003152"
     CDS             complement(1273..2094)
                     /locus_tag="PC621_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF4110289.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2819..3637)
                     /locus_tag="PC621_003153"
     CDS             complement(2819..3637)
                     /locus_tag="PC621_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110290.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3805..4386
                     /locus_tag="PC621_003154"
     CDS             3805..4386
                     /locus_tag="PC621_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110291.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4383..4577
                     /locus_tag="PC621_003155"
     CDS             4383..4577
                     /locus_tag="PC621_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110292.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4741..6483)
                     /locus_tag="PC621_003156"
     CDS             complement(4741..6483)
                     /locus_tag="PC621_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF4110293.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6912..8288
                     /locus_tag="PC621_003157"
     CDS             6912..8288
                     /locus_tag="PC621_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF4110294.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8599..9270
                     /locus_tag="PC621_003158"
     CDS             8599..9270
                     /locus_tag="PC621_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF4110295.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9461..10258
                     /locus_tag="PC621_003159"
     CDS             9461..10258
                     /locus_tag="PC621_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF4110296.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3856 a         1248 c         1581 g         3715 t
ORIGIN      
        1 aatataatca atactgattc tataatatta acactatttt ttatgctata taacaattat
       61 tgaaaatgat ataaatataa tatctcaaat gatatgaata taacatctga atagagggtg
      121 ttttatttgt aaaaaataag tttacagttt gagcgtataa acttatggta aaggagaata
      181 attatgaatt ataagaagat tgcaattata acgggtgcta atagtggttt tggtaaagag
      241 tttctaaagt tattaataaa tgaagaagaa ataacagaaa tatgggcagt tgcaagaaac
      301 aaggcgagat taaatcaatt agtagatgaa tttggaagta aggtaaaagt tttttcaaaa
      361 gatttatcaa aaatagaaga agtaaaagaa attggtacat ttttaagtaa agaaaatgta
      421 tgtataaaat accttataaa caatgctggt tttgcaaaat tctgttcata caatgattta
      481 agtatagatg aatctattaa tatgattgat ttaaatatta gtgctgttgt agtattggga
      541 cttatatgta ttccatatat gcaaaaggga agccatatta tgaatatttc ttcacaagca
      601 tcattccagc cattgccata tcaaaatatt tatagttcaa ctaaagcgtt tataagaaat
      661 tatacaagag cattaaatgt tgaacttaag agtaaaggga taaatgcaat tgctgtatgt
      721 ccaggttgga tgagtacaaa tctatttaag agaggtatag ttgtagctga aaaagggaca
      781 aaaaatttcc caggcatagt gtctccagac gttgttgcca aaaaagcatt gaaggatgca
      841 aagaaaaata aggatatttc tgtgtatggt ataaatacaa aatccagtca tttattagca
      901 aaactattgc cacaaaaaat gatgatgaag gtttggttaa tgcaacaaca tattaaggaa
      961 taagagtttc ggtaattaag tatcagttgg agattcaatc tattatatat tataagaagt
     1021 tttgttgagc aaataataat tgtgacaaaa cggcttatct agtgccgata agccgtctat
     1081 aatgtataaa aaatatattt ttgattttac tttatttaga tattgaagta ttaaaatatg
     1141 taggacttga aattttataa agataaatac tatgatattt tgtaaaaact gagatatgtt
     1201 gatattacat agtttaacta tactgttaaa ttgaagtttt cttattttgc attatgctta
     1261 ttacttaatg atttagacta taatttccat accatcatat gcaaaataaa tgttatcaag
     1321 tttcttttct aattcactgt aataaccata tgattttccc cacatttcgt caatatgtgt
     1381 aatgataatc tttttaattc tataatagtt tttaatttca acaatctcat ctaaagtaaa
     1441 tatttcatct ttaaatggtg tgtcttcaag ttttgtacca tcatttaata taccatcatc
     1501 agaaactaga cttattatta aaacatcagc atcaaagtac atatcactat gagtaaatgg
     1561 cttgggatta cagcaggcat atatgacttt tttttcattc tctttgataa catagaaggt
     1621 tatattcctg tctgggttat tgttaatcaa atcaatatca aaattattta tctttacatg
     1681 cgaagtttca ttaatagaaa tgcaatttat aacatcacca taatatttaa gacaatcgag
     1741 attcgttcgg tttatatctt caattacttc aggaagagca taaaagccaa ttggattatt
     1801 ttctcctgtg ataaagtcac atcctatttt ttcgacaatt ctaataccct taacatggtc
     1861 tgggtcatgg tgagaaattg aaatatgttt tacttccgat attttttgtc tgttacaagc
     1921 tactgtaata tcttcaggtg tgtctatcag catttttata tcatcaatat ataaacttgg
     1981 tcctaatcgt tcatatcttc cgcctttttg cctggcttct tcacatattt tgcatttaca
     2041 aaatgaactt ggaataattg acataccgcc actaccaaca atttttaatt tcaaagttat
     2101 tacaccacct tctaaaaata ttagattata taaataatct acaatcacaa ctgattaaca
     2161 aaattatgag ttaataaatt atatcacatg taaatatgaa agaatagtat gaaattttta
     2221 taagattata ttaaatattt ctcaatgaaa ttgactttgt atatgagccg aatgacttta
     2281 ttcaatatag ataagacatt tttcttgtaa taagatatga ttattctata aggttcatgt
     2341 aattttgata taaaatattg attaataaca tatatgttat tggtgaacat atttctatag
     2401 aatggaatat ataatttttt atatttatca ttgtattatt atacaaagaa agttaaaaag
     2461 agtagagtac aaaggaatta tttgtacttt actctttgtt ttgttaaaaa ctaatttata
     2521 ttagtgattc aaatatttaa ctagagtata agggttgtat ataatcttat aagtaattta
     2581 tctgacaaat taagataata tcctaatcaa attctagtca ttcgaacata acatcacaag
     2641 tgatttatct gacaaattaa gataatatcc taattaaatt gtagatattt atacataaca
     2701 ctaaaagtaa tttgtttaac aaattaagat aatatcctaa ttaaattgta ggtatttata
     2761 cataatacta aaagtaattt gtttaacaaa ttaaaatatt atccaattaa tataaaaatt
     2821 acttcttttt aactgggaag catacctctg ttacccaatt atcaggatta ctatcgttgc
     2881 caggacttac atgataaata ttaaacatag gaccatccac atcatagtta ttatctgata
     2941 tccaattacc aatagcctca caagcgtctg caacttgatt gaaatttcca ttaacaattg
     3001 cagttgcagc agtcacactt ggaacagtct taaatttaac atgttcagta tctttatact
     3061 ttccagatac agcaacttgt acttcaacat ctacagaatc ttctttatat ccagtatcat
     3121 aaaagactgc tttagaatac tttgggggtt caatttgaac attctggtct tttgtctcta
     3181 aaaatgcttg atgccataac attccttcgt cttgataagt tggtataacc cttctaagtg
     3241 tcatcatata ttttggtgca aaattcttta tagttacatc ataatttttc ataatatcat
     3301 ttcctccaat tcttttaata gtggtttcta tttttaatag ttttttctgt gtatcttcta
     3361 attgttcttt tacttgagaa tggtggatat ttagatattt tataagactt tctttatcgt
     3421 tatattctgt caaaatttct tttatagaat acaagccaaa gcccatatct tttagtgcat
     3481 ggattctatt tgtagtagaa agttggtctg cactatagta tctatatcca ttagtgttat
     3541 tcgtatgaga aggagttaaa agtcctattt catcataatg acgtaacata cgaatactaa
     3601 ttttacttag ctttgaaaaa tcgcctatct taaacatgtt tttgtctcct ttataaaggt
     3661 acttttagct taatattaat ataaaccata tcataatgtt agagtcaact aaaattataa
     3721 ttaaattata ttgacatatg atgaaaaaaa tattatactg aacatgttca gaacctaaaa
     3781 tgttaattat tttggaggtc tattatgaaa agtaatgata aagatgctag agaacgtata
     3841 attgaagtta cattgaatct tttaaatgaa gtagatgata ttgaggagat tacagttaga
     3901 aaaattgctg agcgtgcaaa tgttggagtt ggtttaataa attatcattt taaaacaaag
     3961 gataatttgt tgagtactgc cataggagat gtgatgtcaa atataatagc agagctatat
     4021 gacgatagtg tatatactct aagacctatt gaagatttga aaaatttatt aaaaaaattg
     4081 tgtgatactg gattacatta tgaaaaagtg cttccttttg tgttgaatca atgtataacc
     4141 aatggagata tgcaagcaga acttgatatt gtccctatgc ttcgtaaaat ttttggaaat
     4201 aaaaaagatg aaatgagtct aagaatcatt gccctgcaaa ttattttacc aatacagatt
     4261 tctgctttat ctacagaatc atttcagtta tacagtggta taaatataaa gaataaatat
     4321 gagcgagata aattcattga tattttaata gaaaatatta ttggagagga tgttgatgta
     4381 agatgaaaat tataactatt ggttcaagtc ttatcactgt tttacttttt ctttcaacta
     4441 tggtttgtgg gttctggatt aagaataata aggtaacaga tgcaagttct attaagttcc
     4501 atatgaatag tgctattttt acagggatat ttttattgat ttccacaatc cttttaatta
     4561 tatatataaa aaaataataa tattattaat aaataaagta tcagaaatta taaagacaag
     4621 agtcttaaaa atttctgata ctttaccaca taattttatt aaacattaat tgtgtgtgaa
     4681 aaaatatcac ttatttcaat ctaatactaa atcttaagag tgagtataaa gtattagaaa
     4741 ttaagaagca ttttttagtt caagtggatt atctatacca aaataatcat ataaagtaga
     4801 gcttatttcc tctaaatttc catcttggaa aggtaattgt aaatttacct tttctaccac
     4861 atctttaaat ccagcatcag tattttgatt taaaaaatcc atatatacat caacagcttt
     4921 tctgtaatct tcttgtgata attgccatat ctgaagtgat acatcagatg caagaccata
     4981 actcaagtaa taaaaaggaa cttggaagtt atgagataca agtatccaaa aaggagcatt
     5041 tttttctcta agtatattat tggaaactcc atattcttcc tctaaatcaa aaaacaattt
     5101 attgatatca tttagactca tatatggatt cttgtatata atttcttgaa attcatcata
     5161 aagacaagca tcaataatag tatttaaaac aatcgataag tgttcttttt ttatagcttc
     5221 agattgttta ccaaaaaaat catcaaaata tttataaaat agaatttcta aagatgttga
     5281 atgaacttca catacatcta tacttggttg catcttatta tttaagttaa tactgttgta
     5341 gtaattataa aaatgaccaa actcatgagc aaaactagta acacctaaaa aagaattgtc
     5401 ccatgtatta aacaagaaag gtgctttata tttatttata taagtagtat atcctccagg
     5461 cgatttgttt tttccagaag aataatcgta caagtcatat tttttcatat agtcgaagct
     5521 ctcttttagt tttggagata tatcttcaag tactttatca aacttcctaa atgagcgatt
     5581 tttatatact ttaatataaa tagaagaatc agatggttta gatgaaatct ctctatataa
     5641 tggaactatg taatctttta catcttgtct aaatttttta gcttcttctt gagaatagtc
     5701 tttgtttagg ttcatatagg cataatctat ataattctta aatccttgct tacttgctat
     5761 ttcagttctt gtctgtataa gttctaagaa tatgttacca aatataggat tgtatttttt
     5821 taagtaatct gaataaattt tcacatattc ttcagggcta agatttggtc ttgacaaggc
     5881 ttcttcaaag tctacctcta taccatctat tgatactgta gatttagcta ataagtcttc
     5941 atattttaca actaggtctt gctctttttt aagtaaatct tcaacttctt tagaattaag
     6001 ttttccaatg ttctttagat aattaaatgt atcctttcca agttcttttt ctaattcttt
     6061 agagaattta gaagttacaa atctatcttg aaagtctttg tatgacatgt caacatctac
     6121 tgatttactc ataagatact tatattcctt agagtaaaat tcgtctgatg aatctatgtt
     6181 atttctaatt tttgctatag taagtgtact gtaaaaatcc tgataaattt tatcaacttc
     6241 attaaataaa tttaattgtt ccttagctgt tttagctgtt aaaagtttgt cgttacactt
     6301 gttaatgttt tcgcaaattg actgtatatt aggacgctta tattccattt tttcaaaagg
     6361 aatagttcct ctaacagaat ctttttttgt atcattagta taatttctta tgtatgatgc
     6421 aaatataata aaaactgcaa ttaaaattag aaaaacaaat attttcttct tggatttaac
     6481 cataaaaatc ctccaatcat ggatatagtt attaattatt ttactatatt aaatataaaa
     6541 aatgcatgtt aagaaaattt aacataaaga tatagataag attataaata atataaaaaa
     6601 tttaacttca aaaaagtata tgaaatttat actggtaaaa tgataaataa attttaagta
     6661 caaaatagta aaagctatga tagttttaga cccattatat gtaaaaaata tagatgtttt
     6721 tggtgcaatt taaattaaaa gatatatata aaaatttaaa atattccaaa cagaaattat
     6781 aaaaattact atcattgtct ttatgcaata tttatactat aatagaataa taatttagag
     6841 gaaagctcta aaaataaaca aattaagggt cttaacagta gtgattttat gttaaggagg
     6901 tataaagaaa gttgaaaaca atagttagaa gacttattct ctacgcagat ggaatgcgtc
     6961 ctactcaaat aatggtatct gggtttgctg cgattattgt aatcggagca ttgttgttaa
     7021 cactcccgat agcgtcgcag agtggagaga gtataggctt attaaatgct ctatttacgg
     7081 ctacttcagc ggtttgtgtt actggtctag ttatggtaga tacagcaact tattggagtt
     7141 tatttggaca gatagttata ataacattga tacaaattgg tggtcttgga tttatgaccg
     7201 tagccacaat gttttcactt atggcacgaa aaaaaataca actaagagaa agattattaa
     7261 tacaagagtc attaaatcaa gctgatttat cgggacttgt aagacttaca agatttgtat
     7321 taataataac cataacaata gagggaatag gtgcactggt tttatcaact gtctttattc
     7381 cacaatttgg tttgtctaaa gggatatggt atagtgtatt tcacgcaata tcagcgtttt
     7441 gtaatgccgg atttgacttg atgggcagtg taagtggccc atttacatcg ttaaattctt
     7501 atgtaaacaa ctttacagta tcaatgactg tttgcgcact tatagtgctt ggagggctag
     7561 gattccctgt tgtacttgat attgtaagaa aaagaagatt ttcaaaatta aatgtgcatt
     7621 ctaaggttgt attgttttca actgctacac ttatttttgt gggtgcatta tttatttttc
     7681 ttatagaatt taatcaaaaa gctactatgg cggacttgcc tttaaaggga aaagtactat
     7741 cagcaatatt tcaatcagta acagcaagaa ctgctggttt taatacactt gatttagcta
     7801 ctttacgaga aagtagtgta tttgtaatga taattttgat gtttattgga gcatcacctg
     7861 catcaactgg tgggggtata aaaacaacaa cactagctgt tttaatcatt acagttagaa
     7921 gttttctatc aggaaaatca gatatagaag cttttgaaag aagattagct ccatcaacta
     7981 taaaaaaatc tcttggtata tttgtaatca gtatatcagc agttattttt ggaacattga
     8041 ttatttcaat aactcagcct aattttacat tagttcaatc agcttttgag gttacatcag
     8101 cacttgctac agttggttca agtttagctg gaactccaaa tttaaatgct ttaggtaaga
     8161 ttataataat aatctttatg tttatgggta gagttggttc tcttacttta tttatggcta
     8221 tactttctgg tggtagaaga aagagtcaac caatcagata tgcagaaggt aaaattatgg
     8281 ttggttaagt taccaaggag taatataaat ttataatgga tttagagtag cactgtaaat
     8341 ctgttgataa aataaatgag cctagatgta attatatgaa gaaataaaat caactaataa
     8401 acaaaattaa ttaatgaaat aaaaaaacag ggaaataaaa tacaaataaa aaggtaaatc
     8461 aaaattaaat taatctgtgt atataaaatt atatattgtg tataagtttt gtatgtgtaa
     8521 agtatataag ttttgtatgt gtaaacaatt aatttaagag atattatata aattgtgtat
     8581 tagggaggaa gtttcgagat gaagcaatat atagtcattg ggtgtgggag atttggaagt
     8641 tcagttgcgt ctactatgca tcttttagga catcaagtaa tggcaataga caaaaatgaa
     8701 gattcagttc aaagtatatc tgacaaggta acccattcac ttatagtgga tgttactgat
     8761 gagcaagcgt taaggtcatt aggtttaggt aactttgatg tagcagtagt tgcaataggt
     8821 tctgatataa gggcatctat aatggcgact cttatagcca aagaaatggg tgtagagttg
     8881 ataatatgta aggcaaagga tgaattacaa gctaaagtgc tttataaaat tggcgcagat
     8941 agagttgtat ttccagaaag agatatggga gtaagagttg cacacaattt agtttcggat
     9001 aatatattag accatattga acttgaccca gagtattcaa ttgttgaaat cgtaactcca
     9061 aatagttggg ttggcaagac acttatagag cttgaattaa gagctagata tgagataact
     9121 gtacttgcta taaaaacagg taaaaatata aatgttacac cttctccaga tgaggaactt
     9181 acagccggaa gtatcctagt tataatcggt caaaatacta gtataacagc gataacatct
     9241 ggaaataagg ggataattag aagaagataa tttactattt aatatatatt taattgcaat
     9301 gaaagtaaag agtatcatat aattatgaat agttatatga tactattttt atttaatcga
     9361 aggtagtatt tatttgtaag attagataaa gagaagttaa attaaaagta aggaggctgt
     9421 gctaatataa aaatttataa gttattagca tttagataaa atgattacaa atataaacag
     9481 taaggataat gaaaagttaa agtatacaag agcactatta aaatcaaaga ataggaataa
     9541 agagtcaaag ttcataatag aaggatacag aatagtaatg cttgcacttg aatgtatggc
     9601 aaaccttgat tatgtattta tcaatgaaga atttgaaaat aagaaagaac atgtaaaact
     9661 attagaagat ttggataaaa aaaacacaaa gatatacaag actactaata aaaactttaa
     9721 agaattagtg gatacagaaa atactcaagg aataataggt gtagtttcat ttaagaaaaa
     9781 aaaattaagt gaaagtataa ataaaaaaga taaatttgta ttgattttag atagaataca
     9841 agacccagga aatatgggga ctataataag gactgctgat tctgctggag tagatgccat
     9901 aatagcacta aagggatgtg tcgatatata caacccaaaa gtaattaggt ctactatggg
     9961 ttctattttt gatatgaata taattgatgc ttcacaagat gaaactgtgg acatgcttaa
    10021 atcattggat tttaatatag tttcaagtta cttaaataca gaaaattttt atgacaaaat
    10081 agattatggt tcaaaagtag cattggtgat aggaaacgaa gcaaatggaa taaatgaaga
    10141 acttgtatca aagtctgata ttttggttaa gatacctata tatggtaaag ccgagtcgtt
    10201 aaatgctgcg ataagctctg ctatactgat gtatgaaata aaaaaatact taatttaatg
    10261 tattgtaata aatataatgt catgttataa tcttgaatta aatagtagag tatcaaaaat
    10321 agtcaatata tattaaaaaa ataattaaat taattatata ttagatgtat atcatataaa
    10381 aaatgattga gttgattatg
//
LOCUS       DAMKPG010000114        10196 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMKPG010000114 DAMKPG010000000
VERSION     DAMKPG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.119"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            121..2310
                     /locus_tag="PC621_003160"
     CDS             121..2310
                     /locus_tag="PC621_003160"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF4110297.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2378..3145
                     /locus_tag="PC621_003161"
     CDS             2378..3145
                     /locus_tag="PC621_003161"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF4110298.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3203..4219
                     /locus_tag="PC621_003162"
     CDS             3203..4219
                     /locus_tag="PC621_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF4110299.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4285..4533
                     /locus_tag="PC621_003163"
     CDS             4285..4533
                     /locus_tag="PC621_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110300.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4710..5207)
                     /locus_tag="PC621_003164"
     CDS             complement(4710..5207)
                     /locus_tag="PC621_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF4110301.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5448..6512)
                     /locus_tag="PC621_003165"
     CDS             complement(5448..6512)
                     /locus_tag="PC621_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110302.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6515..7612)
                     /locus_tag="PC621_003166"
     CDS             complement(6515..7612)
                     /locus_tag="PC621_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110303.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYEN
                     NLLNEKSNNLSIEKLKSNDNIAILLQDILDTYHEKLYLNNSQKLSSEDFSKLFNKNSI
                     DTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPVTRVQ
                     FLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSISTFITT
                     FFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETFKTID
                     SIESLIFTCLIASTVLIALSIVKEKYKWREV"
     gene            complement(7626..8561)
                     /locus_tag="PC621_003167"
     CDS             complement(7626..8561)
                     /locus_tag="PC621_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110304.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(8749..9393)
                     /locus_tag="PC621_003168"
     CDS             complement(8749..9393)
                     /locus_tag="PC621_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110305.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(9386..>10196)
                     /locus_tag="PC621_003169"
     CDS             complement(9386..>10196)
                     /locus_tag="PC621_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4110306.1"
                     /translation="DKNLYLALESSSTLLIITLFSVYLENENLSKLYNQSLYDKLRVS
                     KDELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKD
                     KPLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSFIKLTNINV
                     RLTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNLIITISDNG
                     IGCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFFE"
BASE COUNT         3866 a         1213 c         1620 g         3497 t
ORIGIN      
        1 agtataatat atattatgtt agataattaa tatgaaaata tacaaatata taaaaaagtt
       61 tgtttagagt tatagttaat gtgtgtatta atttaatact aatttattgg aggtttaaaa
      121 atgaaataca taataaaaag agatggtaca aaagaacttt ttagtaaaag taagattgaa
      181 gatgctgtct ataaagctag tataaatagt gaaggtggag tagatagaga gttggcatat
      241 gatatatcaa gaaaaatagc taatgactat aattctatgg aaaaatgctt atccgtagaa
      301 gaaatacaag atatagttga agtactactt atggaatcaa atagaaaaga tatagcaaaa
      361 cactacatat tatatagaga aagacgctct gaaataagga ctaaagcttg ggagtttgat
      421 gaattacaaa aatcaatatg gaagaacaag tatagacata atggtgagag ttttgatgaa
      481 tggataaaga gaatttcaaa taatagttca agaatatcaa aactaataag acaaaaaaga
      541 tttctatttg ctggaagaat tttagcaaat agaggattac agaaagaagg aaaaaaagta
      601 acatattcta attgttatgt aatagaacca ccaaaagata gcatagaaga tatatttgat
      661 actgcaaaaa aacttgctag aacttttagt tatggtggag gagttggaat tgatatttca
      721 aagctaagac caaaaggagc gaaagttaac aatgctgcaa aacatacgac tggagcagtc
      781 tcttttatgg aactttacag tatgacaact ggcttaattg gtcaaagagg gagaagagga
      841 gctcttatga tttctatgga tgtaaatcat ccggagatag aagagtttat agatataaaa
      901 acagatttgg agaaggttac taaagcaaat atttccataa gggtaacaga tgaatttatg
      961 caagctgtaa gggaaaaaaa gttatataat tgtacttttg aagttgaaac tactggagaa
     1021 aaaatagtta agatagtaaa tgcttatgat ttatttaata aatttgtgaa aaataactgg
     1081 gattatgcag aaccaggtat attgttttgg gataaaataa aaacatataa ttttatgagt
     1141 gaagataaga acttttgctt ttcaggagta aatccttgtg cagaagaacc acttccaagt
     1201 ggaggaagtt gtctacttgg ctcaataaac ttgtcagagt ttataataca accatttggt
     1261 gaacatgcta cttttgatat taataaatta aaatcttgcg ttagagaagc tgtagtagct
     1321 ttaaatgatg tcttagatga ggggttgaac ttgcatccat tacaagaaca aagagatagt
     1381 gtacataagt atagacagat aggtttaggt gtaatgggta tagctgatat gcttataaaa
     1441 atgggaataa gatatggttc tgatgaatct ttagaactat gtgatttaat tggtaagact
     1501 atgttaaatg aagctgtgaa gcaatcagca ttaattgcta aagaatatgg aacatttagt
     1561 gagtataaat atgaaaatat ttctaaatct aagtttttta tagaaaatct aaatgaagac
     1621 actaaagaaa tggttaaaaa gtatgggctt agaaattcac aacttttaac catacctcct
     1681 acaggttcta tatctacaat gataggtatc agtggtggaa tagaacctat atttaatctt
     1741 tcatatataa gaaaaactga aagcttgcat gatgaggatg tgtattataa agtatatact
     1801 ccaattgtta aagagtatat ggacaagaaa ggtattacag atgaagaatg tttaccagat
     1861 ttttttgtta ctgctatgaa tataggatat gaagaaagaa taaacatgca aaaagtatgg
     1921 caaaagtata ttgatgcttc tatatcatct acaataaata taccttttga aactacagtt
     1981 gaagatgttt tcaatatata tattaaggca tgggaggctg gtcttaaagg agtaacaata
     2041 tttagagatg gatgtaaaag gagtggaatt ttaataaatg aaaaaaagga agaaaataat
     2101 aaagaagata aaaaagaaaa tagtaaaaaa gaatattata aaaataataa aaaagaaaaa
     2161 gataataaag atgaaaaagc caaagaaata ataaataaaa attttgaaga agatgaaaaa
     2221 tttgtgtgcc ctgaatgtgg aagtgaagga attgcacata caggtggatg tagtatatgt
     2281 ttaaaatgtg gctatagtgg atgcaattaa actctaatta aaaaatttta atatatttaa
     2341 aaattaaatt tttaaaaacg aaagatgagg gaatggtatg caggataaaa gtgtgaggga
     2401 gattaaagaa ataatagaga ctttagaagt agaaaaatat atggaatata tagaactgtt
     2461 aagagttgat gaaagaaaat ctgtacaagg tctagctata aaactagcaa aaaagctgga
     2521 taatattaga aaagaagaag aaagattgga aactataaat atatttgaga acgaaggcta
     2581 tgataaaggt tatttgtata ttggaggaat tgatgaggcg ggaagagggc cacttgctgg
     2641 acctgttgta gcttctgttg tagtatttaa aaaagataca aaaatagaag gtgtaaatga
     2701 ctctaagaaa ctgagtgagg caaaaagaga tgaacttttt gaagtaataa aagaagaagc
     2761 actagattat ggaataggta tagtaaataa tgaagaaata gatgaattta atatattaaa
     2821 tgctacatat atggcaatga aaaaggctat aaattgttta aaaaaagcac cggattattt
     2881 attagttgat gcagcaacaa taccaggtat tgacatatct caaaatccaa ttgtgaaagg
     2941 agattcaaaa tctatatcaa tagcagcagc aagtatactt gctaaagtta ctagggatag
     3001 tataatgtac caatatgata gagtataccc agaatatggg tttaaatctc acaaaggata
     3061 tggaactaaa gaacactatg aagctattga aaaatatggc ataactccta tccacagaaa
     3121 aagttttttg aaaaatatac tgtaaatata atatgatata atgtatataa tataaattac
     3181 tatagttaat gggggattaa agatggaata taatgaatta ttaaaaagtg ctagagaaaa
     3241 tttaaatgga agttgtaaag tttgtaaggt ttgtaatggg gttgcttgtg caggtgaagt
     3301 gcctggcatg ggaggtaaag gaagtggttc ttcttttata gaaaatagca agagtttaga
     3361 aaaagtaaaa gtaaatatga gagtaataca tgatgtatca aatcctgata catctataga
     3421 gatgtttgga aagaaaatga gtgcacctat atttgcagct ccagtaacag gaactacttt
     3481 aaatatgggt ggaaaaatta atgaaagaga ttacatagaa ccagtagttg caggatgtgc
     3541 taatagtggt atttatgcaa tggttggtga tacggcagta gatgcttttt taatggaaaa
     3601 tttagatgta gtcaaaaagt ataatggagc tggaattgta tttattaaac cttgggataa
     3661 tgaaaatatt ataaaaaaga taagattagc tgaagaagct ggtgcatttg cagttggagt
     3721 tgatatagat gcctgtggtc tagtaacttt atcattacat ggaaaaccag tacttcctaa
     3781 aaatgttaaa caaataaagg agttagtaaa gtctactaaa ctaccattta tattgaaagg
     3841 tataatgact gttgaagatg ctttaatggc agtagaagca ggtgtgtatg caatagttgt
     3901 atcaaatcat ggaggaagag tgcttgactg tacaccagga gcatgtgaag ttttaccaaa
     3961 aattgctgat gctgttaaag gtaaagttac aatacttgca gatggtggag tgagaactgg
     4021 tttagacgta ctaaaaatga taggtttagg tgcagatgca gtattaatag gaagaccttt
     4081 tgtgactgct tcatttggtg gtgcaactga tggtgtagaa acttatgtaa ataaacttca
     4141 atcagagcta agtagttcta tgatattaac tggatgtcaa actataaaag atattgatgg
     4201 aaaagttata tacaaataaa attttataca aaaaagagga aaaatcattt taatgtagaa
     4261 taattgtaca aagggggaat ttatatgagt agaaaagctt atgaagaagc cttagtagaa
     4321 ttagaaaaat ttattgatga aagaaaagaa ataattaaaa gtgcggagga ctgtattgat
     4381 aagtacatag ttgacagaac gctacctttt gattacaaag ataaatgtgt ggaatggcaa
     4441 caagagttac ttgatattgc agaggcacaa gttttagaag caaacgaatt aggtgtttta
     4501 ctggaagaga aaaaagaact tgaggaagag taagttttat tactaaatct agggaaaatc
     4561 aagaatatga tttaccccta gattttttta tttaaataaa gatgtctata taattaatct
     4621 atctttatca atgttagatt aaaaggtttg aagaaaaata gagtaattgt atgaatagtt
     4681 aaacaattaa actagtctat aagaccaatt taattgttat tttttgaatt tagtatatgg
     4741 tttttcattt cttctaatac aattgtgtag gatttgcatt gtgctgcagc caattctgac
     4801 aaagcaaacc tttgagcctt atttagaaag tccttatctg tgctacatag ttgttttgaa
     4861 ttatcttgta tcataagcat cttaattaat tttacaatct caaaaacatc tcctgaatga
     4921 aatttatcat ggtatagttt aagtcgtttt tttctatcaa aaacccataa acaatcaacc
     4981 ttatcaaggg attggataaa gttttcaact tcattttcat ccataattgg acgtacacca
     5041 atcttatcag catttaaaac tggaatcgaa attttaagat tactattgta aataacagtc
     5101 aattcatagt atttctgtat atgatgattt atatcctttg ttacaagacc acttatataa
     5161 caggctcctt cttttggata cattacactt tcaccgattt tatacatatg gttatacctc
     5221 caaactgaat tttagatatt gcaatttggg tacaatgctt taattatcta ataattaata
     5281 ttatatcacg aaatgcttga caagtcaaaa agattaatat ataataaaaa gtttagaata
     5341 atagatatat ataattggct ataaaaatat aagataagag gatttaatat attaaaatat
     5401 ctagttagta ttgtataaaa taacatacta actagattta ataattatta atttctagca
     5461 aaatctctta agttaagact tccggctgtt acaaatacca ttccaataag aattaaaaca
     5521 attatattta ttaatatatt accattatta ttaatagcat ctatagtcca ataaaatggt
     5581 gtaagctttg aaagattagc cataaaggat aatttatcaa gtgatggtat catattagat
     5641 atggatatag ctaaacctat taaagaataa aacgtagaca taagacttat agaaccttcg
     5701 tttttaaaca ccctagctat ccaaaataca aatccagtag caacaaaact atttgctagt
     5761 acaatcatca atgtatttag agtcaattta aaattactta tagatgtgaa acatactaaa
     5821 gttaaggtgt atacaattgc ttgagtaaaa aataacccta aaaatatgct gaaaattatt
     5881 tttatatcat tatgctttgt agaaacaagt ctttttaagg tattagattt ctttaatgaa
     5941 aataaatcag aagctagatg tgctgcatta atgtacatga aataacatat caagaaaact
     6001 attaaaaatg aatttttatt cttacctgaa ctgttatcaa ttatatttgt cttggtcaat
     6061 ttatcatcta tatttctatc tatttttaat tttaatgaat tagtaataaa agattctatt
     6121 tgtgattctg cccaaagact acctccacca ttttcagttt taaaacattt aacctcaggt
     6181 cgttttaagt tatctatatt gcttgaaaag tttttatcta aaatgaagac agcagaaact
     6241 ttattatttt ttaatagatc catagccttg tcttgttcgt ttaatccaaa tgagttttct
     6301 tttaatttag aactatctat taacttatgt tcgtattttc cagaagaatt ggatacaatc
     6361 gctactggag aaaagtacat tgattgattt tctgagttgc cagtaaaaaa gttaagataa
     6421 aacattaata taacaggaaa caaaaccata ttaactaaaa gagcatgatt ttttatatat
     6481 ctttttatgt ttgcaatagt caacctaatc attattaaac ctccctccat ttatattttt
     6541 cttttacaat agaaagtgca atcaatactg tagatgcaat taggcatgta aaaattaaac
     6601 tttctattga atctatagtt ttaaacgtct cataattact gaacaatgtt cctattaaat
     6661 aagtaggaga taaacttgta aacttagtaa acacatcatt tactatgctg aaaatacctc
     6721 caaaaactgt ttgcaagaag agtaatgcat aaactataac tgtaccatat ttcttgctaa
     6781 aaaaggtagt tataaaagta gatattgaag tcataaaata agaagctaaa gcacaaagta
     6841 aagctaatat aacaaagtta cctttgaatg ttacaccgag tgttctaaaa aataaaacgt
     6901 ataatgataa aaatataaat gagtaaatcc agaaacctat aaaatcataa attaaaaatt
     6961 gtactctagt tacaggtata gagtagagtc tttttgaaag tccttttgat tcacttacat
     7021 aatttgataa aatgctattc attataaaaa ttattgcgaa gaatccaagg gaaactaaag
     7081 caaagtactc ataagagttt tgtttaacat tattttttat tatagaggta tcaatagaat
     7141 tcttattaaa taattttgag aaatcttctg aacttaattt ttgagaatta ttcagatata
     7201 atttttcatg ataagtatct aatatatctt gaagtaatat tgcaatatta tcattagatt
     7261 taagtttttc tatacttaga ttatttgatt tttcatttaa taaattattc tcatatcctt
     7321 taggtatagt aagttttaag tctgccttag aatcatcttt ttgaactgtt aatatagaag
     7381 ataaatcgtt ttttagatat cctgtcaaat tacttgatag ctttgtatta tctttatcat
     7441 atataacaat tgatgtcaaa tctgttttta taggattttg aaatatactt tctgtcacca
     7501 tcccaagaaa tccagctaaa actagaggta aggctaagaa atatattaaa ttaagcataa
     7561 aggagctttt taaaactttt agcatagttt ttacactaat gtacaatttc atgaaatcac
     7621 ctccattagt cccttaaatt tttacctgtt agatttaaaa atacgtcttc taaactaggt
     7681 tcttcataag atattcgtgt tatttttgaa ttatttttgt caacaacaga taatacatct
     7741 gtaagttgta attttttgtc tacacctaat tctaataatg aattattttc taaaatagat
     7801 acaacacctt tagtattttt taaatcaaat ataagttgag catttatatt ttttatttcc
     7861 ataattaatt ttgtatttgt agatactaat gattttaaat aatcattatc tccaaaagca
     7921 atctctttac cttcatccat aataaaaatc ttagaacaaa gatgctcaac ttcttccata
     7981 taatgagaag tatatattac tgtggttttt tgctttttag atatattttt tataaaattg
     8041 aaaatatggt ttcttgattg cgggtcaaca cctacagttg gttcatccaa tattaatatt
     8101 ttaggatgat ttaaaagtgc tattccaatg ttcaatctcc tttgcatacc accagataat
     8161 tttttgattt ttttcttttt aacatcagtg agtcctgtga cttctaaggc ttcagttatt
     8221 ctctcttgaa gcaattttcc ctttagtcca tagtaagtgc caaagtactc taagttatca
     8281 aaaggagtaa gagtctctat aagtgctaat tcttgtggaa ctaatcctat tatagacttt
     8341 gcttttatac aatcttttga caaatctaaa ccattgattt ggatgtcgcc actatttggt
     8401 aacataagat ttgtaattat atttataagt gtagatttac cagcaccatt cggtccaata
     8461 agtccaaata tctctccttc tttaacagaa aagctgatat tatcaagtgc taatttatca
     8521 ttaaatcttt ttgttatatt atttactctt actatatcca taataattcc tcccaaaata
     8581 attttgtata agttaaaaat ctataatact ataataaaga attatgaatc ataattctat
     8641 tactataagt cactaaaaaa agtgacttaa gtcactttat attttcattt attataaatg
     8701 agtttgtgag taagaaattt ctagggtata gatttcttac ctatatattt atattacaat
     8761 tttatttttg aaggcaaata taactagttg tgtcctatca cgaagctcta gtttccctaa
     8821 gatattggtg atattattct ttattgtacc ttcagataag aataattctt ggctgatttc
     8881 tttgttgtta agtccatttg cgattaatct aattatggat atttcttttt ctgttaaatt
     8941 atacatatca gaaatatgtt tttgttctat agttttttga gggtacataa ttttttcagc
     9001 cacactttta tcaagtacaa tatttccaaa aaacgaggaa cgaatccctt catgtaatac
     9061 ttcatccgaa ctatccttta aaagatatcc tgaagcccca aatttcatag catcttctat
     9121 gtactcatca tcattaaagg ttgtcaaaat taataatgct atagagctat catattcttt
     9181 tataagtctt gtgcctagaa ctccatcaca aactttcatc cgaatatcca taagaactat
     9241 gtctacatca tttgttttac aaaattctaa tgcttcatat ccattggaag catctcctac
     9301 aatttctaaa tcgtcataag atgataaaat aatgttaaga ccttttctaa tcaatttttc
     9361 atcatctact ataagaactt tgattttatt caaaaaaatc acctcctgaa aatttaggaa
     9421 aagacgctct aattgtgaat ccagtattag aattatcgaa ttcaacttta ccatttaatt
     9481 catatattct ttcgctaata ctgctaagtc catttcctct ttttatattg ttacacccta
     9541 taccattatc gctgatagta attataagat ttgaaggatt aaatgttata aaaattctga
     9601 tttcagttgc ttttccatgt cttgatgcgt tggaaagaga ttcctgaaca agtctatata
     9661 aagcgataga ttgatttgta gataggttcc aggtattttt tgatatagtt aatctaacgt
     9721 tgatatttgt aagtttaata aagctattaa ccagttctct gattttaagt aaattttggt
     9781 aattttcata ttcaatgggt tttagttcag atatagctct tctcacatct tgaaaacttt
     9841 cagttacaaa ctctctaagt tcatgaacta aatcagaaat gagaggtttg tcttttaaga
     9901 gtttttctat agcatttaat tggattatcg tcgtagaaag agaatgtccc acactatcgt
     9961 gtatttccct cgatatacgg tttctttctt tcaaaattgc taactcttct atagaatttg
    10021 cataaatctc taaatcggca ttagcttttt ttaattcatc tttagaaacc ctgagtttgt
    10081 cataaagact ttgattgtat aatttagata aattctcgtt ttctagatac acagaaaaga
    10141 gggttataat tagtaatgta gaacttgatt ctagtgctaa atataaattt ttatct
//
LOCUS       DAMKPG010000115        10141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMKPG010000115 DAMKPG010000000
VERSION     DAMKPG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.120"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            216..926
                     /gene="rgaR"
                     /locus_tag="PC621_003170"
     CDS             216..926
                     /gene="rgaR"
                     /locus_tag="PC621_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF4110307.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1972..3003
                     /locus_tag="PC621_003171"
     CDS             1972..3003
                     /locus_tag="PC621_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF4110308.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            3136..4434
                     /locus_tag="PC621_003172"
     CDS             3136..4434
                     /locus_tag="PC621_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF4110309.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4548..4793)
                     /locus_tag="PC621_003173"
     CDS             complement(4548..4793)
                     /locus_tag="PC621_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF4110310.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4941..5807
                     /locus_tag="PC621_003174"
     CDS             4941..5807
                     /locus_tag="PC621_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF4110311.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5811..6347)
                     /locus_tag="PC621_003175"
     CDS             complement(5811..6347)
                     /locus_tag="PC621_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF4110312.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6537..6740)
                     /locus_tag="PC621_003176"
     CDS             complement(6537..6740)
                     /locus_tag="PC621_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF4110313.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            7031..7795
                     /locus_tag="PC621_003177"
     CDS             7031..7795
                     /locus_tag="PC621_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4110314.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8184..9497
                     /gene="prdC"
                     /locus_tag="PC621_003178"
     CDS             8184..9497
                     /gene="prdC"
                     /locus_tag="PC621_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF4110315.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            9666..9828
                     /locus_tag="PC621_003179"
                     /pseudo
     CDS             9666..9828
                     /locus_tag="PC621_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4069 a         1003 c         1654 g         3415 t
ORIGIN      
        1 tattatattt attttactat atttttttac ataatatatc tagaaaagta taggaattta
       61 tcgtaattta agtttaaata ttaaagagaa tttctataag tagtttaatc gacaacttaa
      121 atttataaag aaatttaaat ttgaaagcta gtgagtacag tttggaaatt gaaataaagt
      181 aaactaaatg atattttaga tttggaggtg atattatgtt cagggtagtt atatgtgatg
      241 acgaaaaaat acaaagaagc atactaaaag agtttacaaa aaaacttttg tctgaaagat
      301 gtctaaatta tcaaatactg gaattttcct gtggagaaga tttaatatcc aaatacccag
      361 aaaaaattga tattatattt ttagatatcc aaatgaaaga tatcaatgga attgaggtgg
      421 ctaaaaaaat aagaaagttt gatgaaaaag tagaaattat atttacaaca gctttttcag
      481 agtatgcccc ccaaggttat gaggttcgtg cttatagata tttagtaaag cctatagagt
      541 atagtaactt tgcaatgggt gtaaatcttt gtattgataa ccttcaaaga aagaaggagc
      601 gttatatagt tttaaatagt aaaaaaggat ttaatagaat actgatagat tctattttat
      661 atgttgaaac tgtaaagaga gatttagttg tacatacaat aggtagagat tataaagcta
      721 atatgagcat gaaacaagca gaaaaattac tgtgtgaaaa tggctttttt agatgtcata
      781 caagttttat tgttaattta agaagaatag aagatcttag ggataatatg attactatta
      841 atgaacaatt tatacctata agtaagtata gacttaaaga tttaaaagta gcattaacaa
      901 gcttgttatg tgatataatg tattaatttt aaaaaggtaa ttgaaaatat cgtaaaaaag
      961 atttaatgtc atcacagggc tattgattaa aactgtctta atatataaaa tgttatttat
     1021 attaagacag ttttattatg caaaatatca gtttatagac ttttaatatt gtttaatatg
     1081 tatatgtaga gtttccatta cagtagcatg attattaatt agtagcctga ttattaatat
     1141 actattattg atttttatat gcattatatg tatttttaga atatgattac ctttttaaaa
     1201 taatgactat ttataaaact ctatagatat ttgtttcatt tagctatgca tactttaatt
     1261 aattatatta atatttaatg gagtataata taaatattaa agcgacatct tatagtttat
     1321 tgaacctata gtaatgaatt aataaatggt ttaattctat gaaaagattt ccttttatgc
     1381 aatttattta ttgaaatatg atataatata taaaggtgta agtattagtt gttaaaataa
     1441 tatttatatt agtttttatg catacttggt ataagttttt catatattgg ttaaaattga
     1501 tgttgattta ttatttttag tattttttca ccttttgatt catactttgt agaatggtgt
     1561 attttataaa actgattata agacaagttc attcaaatta gcatttttat atcatataaa
     1621 taattgttcc ccctaaataa gatttagatg tatagtatta aaatcaaaaa gtataaaata
     1681 aaattgatgc ttaatcatag ataaatatta ataaaatata caaggggatt gtctaataaa
     1741 aagataatct ccttttttac aaataatcct aaattgtgat ataaatctaa aaattttatc
     1801 ttaacattta accatttaat gataataatt gatataataa atataaatta aatatatggt
     1861 gtacttattt tatgttttta gcaagaaata tagcatttaa aagataaatt gtgcaactta
     1921 aatcaaagtt taagatgaac tattaacata gatatattaa aaacttttgt aatggagaat
     1981 atatgtgata tgaataataa agttaaggtt gcagtattag atacaggaat agacaaagaa
     2041 catgattact taaaagataa tttagttgga ggaattgctt ttgaatgtat gcatgattat
     2101 atctttatat cagataaatt tgatgatgaa gatggtcatg gaactgcttg tgcatcaata
     2161 ataaagaaag agtatgaaga tgtagagtta tttgtaataa aaattttgag aaaagatggt
     2221 attacaaata taaaagttct tgaagaagca ttaagatacc ttttagacac taacataaga
     2281 ttaattaatt taagtttatc agtcatagga gtagagagtg ttaagggctt atttgaagtt
     2341 tgttacgaat tgtttagaaa aggaaaaata atagtatgtt cactcgctaa tgattttgat
     2401 ttaagttatc cagctatgtt taacaatgtt ataggtgtaa gaggatttac tttagatgga
     2461 gatgattcat tttggtacaa caaaaaatat gatgttcaat gtgttatgga tagcaattca
     2521 tatataagtt gtgatataaa taattcctat agattaccac caaaatgtaa tagttattta
     2581 tcagcaaagc ttactggtaa aatagctaag atattatcag aaaaaccagg cataacaata
     2641 tctgatttaa atgataagct tgaatcatta gcaactagaa atcattggga tagttgtgat
     2701 tttgatgagt gtagtagaat tgtaaacttt aaattggatt tgtatgacaa agaaaatgct
     2761 cttcttatga aagttgctga cgttgttagg gattgtttag atattgaaga taataatgaa
     2821 aaactcttta agggtagcct ttttaataag gagataggtt tggtttatga taattgtttt
     2881 aatctgttga aaaagctaga gcatagattt gacataaagt ttaattatat ggacatctca
     2941 aaatatgact tagtttcaat atatacactg actgaattag tggaaagata taaaaactta
     3001 taaataaaga tttgtagcta aatttaatgt agaaggtatt aaattatatg tattctttgt
     3061 cggtagatta aaagtaaaaa atatactata attagatagc ataagagaaa atgccaatac
     3121 agggggagtt agataatgaa atatgaagaa gcattagagt acatatcaca aactaataaa
     3181 ttcggtataa gattaggatt agaaaatatc ggaaagttat tagaattatt aggaaatcca
     3241 caagaaactt tgaatataat acatgtagct ggaactaatg gaaaaggttc tgtgtgttct
     3301 tttgtttcta atatactaag agagtgtgga tataaagttg gtttatatac atctccatat
     3361 ttagaaactt ttactgagcg tataagagta aatggacaaa atataccaca ggatgatgtt
     3421 gctagaataa ttgagttaat aaaagaaaaa atagagatta tggtaaaaga aggctatgca
     3481 tatccaacag aatttgaagt ggttactgca atggcatttt attactattc agaacaaaag
     3541 gtagactttg tggcattaga agttggttta ggtggcagat atgatgctac aaatataata
     3601 acaaaatcac ttgtaagtgt gatagtatct ataagtttgg accatacagg tatacttgga
     3661 gatacaatag aaaaaatagc gtacgaaaag gctggaataa taaaggaaaa tggagtagta
     3721 ctagtatatg accagactga tgaagcgaag gatgttataa agtcagtttg taaagagaaa
     3781 aaagctaaat atatagaagt tgattttgat gatataaaca taaaaaaatc agatataaat
     3841 tctcaaatat acgattgtac tgtaatgaaa gaaacttata gagaccttga aataaaattg
     3901 ataggtgaac atcaaataaa caattctatc ttggcaatta gtgtaataaa atatctaaaa
     3961 gacataaata aattagcaaa tataaatgaa gaatctataa gaaaaggact tataaatact
     4021 aaatggccag gaagaataga aaagataaaa gaaaatccaa tttttataat agatggtgca
     4081 cataatgaag atggagcaaa atctttggca aaagcacttg ataagaattt taaaggtaga
     4141 aaattaacct tattaatagg tatgttagaa gataaagata ttgatagtgt tttagagata
     4201 ttgttgccac attttaataa ggtaataaca acaacaccaa ataacccaag ggccataaat
     4261 tcagatatat taagagaaaa agtattaaaa tatgtagatg atgtaacatc aaagcatgag
     4321 atagaggatg cagtaaatta tacattagaa acatcaagtg aggatgatat tataataagt
     4381 gcaggttcat tatatatgat tggtacagtg agaactttag taaagaaatt ataattttat
     4441 aaaataaata tacaaataaa gatataaaaa agagttgtct aaaaaatgga caactctttt
     4501 tatataaagt aagtttttaa taattttact gatgtttata taagtaatta tatagattga
     4561 cgtatagcct tagataattg gtctgggcaa gaagtacctt ttccaccaca gtcaataccg
     4621 tttaatttat cagcaacttc aagagcattt tggccgatta ttaagcttct aagtcctgat
     4681 aaattaccat tacatcctcc tataaactca gcatcaacaa tcacattatt ttcatcaact
     4741 tcaaaaatca tttctctaca acatacacca cttggattaa atgttacttt cattaaaaag
     4801 acctccatta aatattattt ctttttttaa caataataat aataccaaat atatgcgtaa
     4861 taatgcaact ataaagcata atacaagcta atgaaataat atattattgt agtatatgta
     4921 ttgtagagag gaggggttat atgaaaatct atatttctaa ctacttatca aagagtatta
     4981 gcatagttga ttattctaca cttgaactag aaaaagaaat agtattagaa gataatatat
     5041 atccacacca tttttgcata gaaaaagaaa aaaatttaat atacatacct agttcaagta
     5101 atgggatact ttatgtctta gatttaagta atgataaaat aattgatact gtctctatag
     5161 gaggaagtct tagtcaagtt gtacttagtg ataatgaatt gtttgtggca aatgaagatt
     5221 ctaatagtat atatgtacta gataaaaaca cattaaatcc aataggaatt attggagttg
     5281 ataatatgcc acatggattt gactttgata gagaaagtaa aaaattatat gtaccgtgta
     5341 taaactcgat agtatgtata gatacaatta ataaaaatat acaaaagaaa ataaatatgg
     5401 actttagagc ttggcatata gagcttgata aacaaaagaa agaaatgtat atatcaacac
     5461 ttgatggaaa gcttataata atagatgaag taagcatgga tataaaaaag gtattatatg
     5521 aatttttatt accagtagaa ataagattta attatagtgg caaaaaagtt tatgttgctg
     5581 atttgggtta taataatgta agaattttag attatacaac gagtagatac attggaaata
     5641 ttgaaataaa tgggattcct caaggcttag aaatatctaa agatgaaaag ctactatttg
     5701 tatcagatac acaagaaaat tcagtaaaag tttatgaaac tgcaaacaat aaattaataa
     5761 aagaaatcaa ggtaggaaaa gagcctacaa ctataatttg tttgtaggct ttaatgtatg
     5821 tctcttataa gtaaatcgat aacttcggca tacatataag atgcattatt tttccagtta
     5881 ttgcaaataa gttttgcttg tttctctgat gaaacattta gatttaaatc tattaagtta
     5941 gactgatttt ctatgactct aaggttgaca ataaattcat tatcactttg ttttacaaaa
     6001 ctagacttta cttgagtatc tgctagtaga ttttctttgt tagaatctat atacttgtct
     6061 attttttcag taacactagt aggtattcga ttaaagaagt attctagact ttctacgcct
     6121 ctttgagtaa gagcataata ttctctgtca gagtctttat atgtagtcaa aaacttagat
     6181 tccataagct gagataaaag ctgttgtaat gaaaaataat tcatcatctc agtctctaat
     6241 actacttgag ttatttgaga attagttaaa tccattttaa ttttttctaa tatatataaa
     6301 attaaaagtt tgtgataggc taattcttca gacgagtttt caaacataaa tatcatcctc
     6361 cgtcaaacta tttctatata taatatatta aactaatttg tacaaaatta aaagaacaat
     6421 ttgtattgta ttattaatct ttaataattc atataaaaca attttataaa taaatataga
     6481 gaaacagggc tgctaattag cagccctgtt gatacccagg tgtcaaaatt tactatttat
     6541 tttccagcca tttgtctttc agccatttca actaatttct tagtcatata tccaccaaca
     6601 tatccatttt ctctagctgt taagttacct ttatctatgt tttcatagtt agataaacct
     6661 atttcgttag ctatttctaa tttcatttgg tttaaagcag cttttgcttc tggaactact
     6721 gttctgtttg aatttgacat agtaaatttc ctcctcaaat aattagttta tgatgtctaa
     6781 caacactttt atttatgtga tgttaatact aatttctcca aaataaaaat aatatataca
     6841 gaaaaataaa ggtaaaaatg taaaaaatat aaaaaaatta aataaatgag tattttagag
     6901 agtttaatta ggtcaatata ggttttattt ttttagttat tgtcttataa attaaatatt
     6961 acagtacttt tttgagctga aaaaaaataa attttaaata tagaagaaat tatttcaaag
     7021 ggggaatagt atgaaaagag aagttaaaag gactaaagct ttgatatata gtgttttatt
     7081 tttttcatta ttattagtgt taatatttgt ttttgtttat ggaataaaat acatatgttc
     7141 taaatacgac aattcaaaaa tgcctatata taaagttgat acaaatgaga aaaaaatagc
     7201 cttgagtttt gatgtagcat ggggaactga taatatggat gatattttaa aaattttaga
     7261 taaacataat gtaaaagcta ccttcttttt agttggtagt tgggtggatg ataatgaaga
     7321 aattgtaaaa gctatagatg aaaaaggaca tgaaattgga aatcattcaa acactcatgc
     7381 caatttgaag gaaatatcaa aagaagacat aacaaaagag atagaaacta catcagaaaa
     7441 aatatataat ataacaggaa aaaagactaa tttatttaga cctccttttg gggacgtaaa
     7501 caataaagct atggacattt gtagtgattt aggttataaa gtaattaagt gggatgtaga
     7561 ttctatagat tggaaggagc ttggaccaaa tcatgtaatt gagaaagtaa taaaagaatc
     7621 tcaacctggt tcaatagttt tattccatgc taatataaat gatgtagata actatttgga
     7681 tactataata agtcgactaa aaaaagatgg atacagtcta gtcaaaatat ctgatttgtt
     7741 gtataaagat aattatgtag ttgattccaa tggagtgcaa aaaactaaga attaataagt
     7801 aataaatgta tattttaagt aaagttgtga taaattatat agtatatgtt agaaaagtgt
     7861 cgataaaaag acggtgtcta ttttttgaca ctttttatac atgtatgcaa tatcaataat
     7921 tatagattaa attgattaaa aaagtgtcta ataattgact tttttttgat ggatttaaaa
     7981 atttagaaga attaatgaat gctgtaagac agtatttcca aagggtataa gtttgtttaa
     8041 aagttggcat agaaattgct ttgttaataa gcgatatgtt ctatagaaat aaacaatatg
     8101 ttaagacctt ataatagaaa aatagcatta acattatttg aacagcttat aaaaaaatta
     8161 atatacttaa gggggcgtac tagttgaaca aactatattc tattttatta aaacaacacg
     8221 ttggtggtcc agataagcca gtagtaagtg ttggagatgt agtaaaaaaa ggaactctaa
     8281 tcgcagaacc aactggatta ggggcaaata tttatgccag tgttagtgga aaaataagtg
     8341 aaataaatga ccaagcgata gtaattgaag ctgatgaagt acaagaagat acttttgaac
     8401 cattaaaagg agaaggtata cttgacttaa ttaaagaagc tggagtagta ggaatgggtg
     8461 gagcaggatt ccctactcat gtaaaattaa acatagattt aaatggtgga acaatattag
     8521 caaatgcagc tgaatgtgaa cctttattag ctcataatat aaaagaaata gaagaaagac
     8581 cagaaatagt ttatcaaggt ataaaatatg ctatggaagt tactaatgct ggaaaaggta
     8641 tgctagcaat aaaaagcaag catccaaaag caatagaagc tttcaaaaaa gtaataaagc
     8701 caggagacaa catagaagtt gcagaactag ttgatatgta cccaatggga gaagagagag
     8761 caatagtaag agacgttcta ggaaaattac ttgagccaac tcaattacct tcagaggcta
     8821 atgcagtagt aataaacgta gaaacactaa ctagaatagt agaggctgtt gagcaaaaaa
     8881 gaccagttat atctaaaaat ataactgtag ttggtcaatt aaacagtgga aaagagtcta
     8941 tagtatttga agatgtgcca attggaacaa ctgtcggaga attaattgaa agagctggtg
     9001 gaataaaagg tgaatatggt gaaataatcc taggaggacc tttcactggt aaagctacta
     9061 ctttagatgc tccaataact aaaactagtg gtggtataat agttactatg ccatttgtta
     9121 atgaaaagag aaaaatgggt cttttagtct gtgcatgtgg acctaatgaa gagagaatga
     9181 gagatatagc aactaaaatg ggagttactg atatagtatc agttcaaaaa tgtaaacaag
     9241 ctcaagaaat taaaggtgca ttaaaatgtg aaaatccagg acactgtcca ggacaagctc
     9301 aaaaatgtat tgaatttaag aaagctggag cagaagttat attaatagga aactgtactg
     9361 actgtagtaa cacagtaatg ggttctgcac caaaattaaa gttaggtgta tatcatatta
     9421 ctgaccatgt aatgagaaca gttaatcacc cattaataag aagaataaaa ggtgacgtaa
     9481 gattaaaaat aaaataatag ataaatagaa gttagacatc tttagaagta aaacttaaaa
     9541 attacttcta aggatgtttt ttaatgtatt ttgagggcaa tatatagctt taaaagaaat
     9601 caaaaaagtg ccaaatgtaa aactaattaa accatatata tttaagaagg gattgaattg
     9661 gagttgtgaa ttatgttagt agaaaccatt ctaagtgttt gttattggta catttgatat
     9721 ttgtatgtaa atacagaaaa aactttttgc tcagatggat actttgcttt cagtgtagga
     9781 aatgtttcta aagaaatgat tgaaaaatac atacaaagta agggataggt ttacatccac
     9841 caacctaaag gtatagtggt tttacgcctt aatttataaa aagaaattaa tttatattat
     9901 tattaagttt aaaataaaaa gtctcaaatt gatttataag tcattttggg acttttttat
     9961 gtaaatataa tatgcaaata tagtttgttt aaaaatatct attttaatta tattcttaaa
    10021 tttatgattg gatacaggca ttcattaatt gtttgatgaa tagaaaaatg ttttgaaggt
    10081 gaaaatagta ttgaaaaaat aatatactaa gggtataatt catttgattt catttgtata
    10141 a
//
LOCUS       DAMKPG010000116        10029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMKPG010000116 DAMKPG010000000
VERSION     DAMKPG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.121"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            71..1552
                     /locus_tag="PC621_003180"
     CDS             71..1552
                     /locus_tag="PC621_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4110316.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1585..5022
                     /locus_tag="PC621_003181"
     CDS             1585..5022
                     /locus_tag="PC621_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF4110317.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5040..5786
                     /locus_tag="PC621_003182"
     CDS             5040..5786
                     /locus_tag="PC621_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF4110318.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5735..6055)
                     /locus_tag="PC621_003183"
     CDS             complement(5735..6055)
                     /locus_tag="PC621_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF4110319.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
     gene            6846..>10029
                     /locus_tag="PC621_003184"
     CDS             6846..>10029
                     /locus_tag="PC621_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF4110320.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIANTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRG"
BASE COUNT         4071 a         1183 c         1565 g         3210 t
ORIGIN      
        1 atacttatta atagtaaaat aaagtgaatc aattatatta taattgctaa agagagtgag
       61 gtgtagctat ttgaaaaaaa atagattaat aataaagaat atatcaatag catttatagt
      121 actattcttt tttagtgtat ttacattctt ttatataggt aatattagta gagtattaga
      181 atatgaaaca aatgacataa tgacagttac aattactgga tggattatac tttcattttt
      241 atttttatgt attataattt acatattata ttcaaaagca aagagtcaaa aaactataga
      301 aaaagtagca tatacggatt ttgtaacagg ttattccaac tggagaaagt ttgaattaga
      361 tgttacaaat ttattaaaga aaacatcaca aaataataaa tatgcaatgg tcatattcga
      421 tattgataaa tttaaagcaa ttaatgatat atatggacat aaaaagggca atttaatatt
      481 aaaagatatt gcagatactt taaatgaatt gactaatatc aatgaaactt ttgctcgtgt
      541 aagtgctgat aattttaata tattattaac atataacaaa aaagaggata taataaatat
      601 tattaaaaaa attatagcaa ataatgaact tgtaaactta tcttttggaa tttatgaaat
      661 caaagataag gatttatctg taagtgtata tagtgataga gcttcacttg caaaatcatc
      721 aataaaaaat aatagtgatg taaattttgc attttttaat gataaattaa gagagaaatt
      781 attacttgaa gataagatag aaaaagaaat ggaatatgct cttgaaagtg ggcaattcgt
      841 aatgtattta caaccaaaat ataatattaa attagataaa ttttgtggaa gtgaagccct
      901 agtcagatgg caatatacag aaaaagaagt catatatcca ggtgatttta ttcctatatt
      961 tgaaaaaaat ggatttatta gaaaaattga tatgtacata ttagaacaag cttgtaaaga
     1021 aatccggtct ttatttgata aaggaatttc acctttacct atatctgtaa acttctcaag
     1081 agttgatttt ttaaaaaaag attttattga aaacatagta aatatatgtg atagatataa
     1141 aataccatat agtttaatag aaattgaaat tactgaatct agtatgtttg gagatactga
     1201 tactctattt aatgttagta gaaacctaca ggatattggt tttatagttg caatggatga
     1261 ttttggctcg ggatattctt ctgtaaatat gcttaaaaat ataccattaa atgtaataaa
     1321 attagataga ggattttttg ttgatgataa agatgttgat aagagtcaaa ttgttataaa
     1381 aagcatagtt tctctcatta agcaattagg aataagggtt gttgctgaag gaattgaaac
     1441 aagaagtcaa atagaaatgt taaaaaaagc aaattgtgat attgttcaag gatattattt
     1501 ttcaaaacca ttaccaatta aagaatttga aaaattagtc tataaaatat agtcagcgag
     1561 tcttaaatgg gaggtggtga ttaagtgata agagcaaaat cgttaaaaaa tgtactgagt
     1621 attgttttaa tatttttatt tacaataatt tctttttctg attttgcatt taaaactaat
     1681 gtccaggaag aagaaaatag taaaattaat ttaagggaaa tatccctaca aagttccaat
     1741 gctataaaaa taaaattaga tagttctcta tcctttatta aaagtgtggc aaatatggtt
     1801 tcttatttta atactataga taactcaaaa atagttccat tattagctaa catatcaaaa
     1861 gaatctgact ttcaaagaat ctctattgta tctcctgatg gaataagtca taccagcgat
     1921 ggcaaaatta tcaatgtgtc aaaatatcag tattttaaga aagctattaa aggagaaagc
     1981 aatatatctg aagttgtgaa atctccaatt gatggaaaag atacttttgt tatagctact
     2041 cctatattta ataagaataa caaaataatt ggtgttttat acggttctta ctacaatgat
     2101 aaattagctg aatttataga tataacaagt tttaatagtg aagggtacat agatatattt
     2161 gaatctagtg gtcaatttgt tttaaaatct tcacatacaa acaaactgat taagtcaagt
     2221 agtaacatat atgatatatt taaaaaagct tcttttgatg atggttatag ctataaaaaa
     2281 atatatgaag atacaaaaaa taacaagagt ggctttacct catatgaata caaaggagaa
     2341 aatcgttatg ctacttatac acccattggt gtaaatgact ggtatgtaat ttctgtgata
     2401 ccacaatata ctgtaagaag aaacattgat aaaataaatc aattagcatt ttctcttatt
     2461 ttcaaagtat tcttattgtt tattatgctt ataatatata ttatttataa aaacaataaa
     2521 tctcagaaag atatactaaa agctaacaaa gaaatctcat ctattacaaa tagcattcct
     2581 ggaggagtac aaaagtgtac cctgggtgaa acatgcaata ttaatttttt aagtgatgga
     2641 tttatagagt tgtttggtta tacaagagaa gaaataaaaa cattgtttaa taatgatttt
     2701 tatagtttaa tacataaaga tgatgttgaa agaataaaaa aagaaatttc taaacaactt
     2761 tacaaaaata aaactattga attagaatat aggattataa caaaaaatgg taatacagta
     2821 tgggttttag ataaaggaga acttataaca gaacctgatg gaaaagaata tttttgttgt
     2881 gtaactgtag atattactag aacaaaacaa ttagaacaag agatggaaca aaaaaatcta
     2941 gaacttgaaa tgatatcaga aaatatttta ggtggtataa ttataacaga atttaatgat
     3001 gattttacta taaaatacac taactctggt tatctttcaa tgattggata tacacgtgaa
     3061 caattagaaa atgaacttaa atacaaagct tgtaatcttg ttttttatga agatagaaag
     3121 aaggctcttg aaagtataca acatcaatta aacacaaaag gaatagctgt atatcaacat
     3181 agaataagaa gaagagatgg taatattatc tgggtttcta taaaaggaag gcttattatt
     3241 gatgatgatg gtattgaaaa aggtatttgg attctaaatg acataacaga tttaaaaaat
     3301 gcagaacaaa aacttaaaat aaatgaagaa agatttagaa ttgcattaaa gcaaacatca
     3361 aatactatat ttgagtatga tataaaaaca aagtcacttt taaatactag taaagagctt
     3421 gataaatata atatttataa aattactaaa aacgttccat attctctagt agaatcagga
     3481 gttattcata aagattttag tgatgatttt ataaacatgt atgaaaatgt aataaatgga
     3541 gcaaaaaaag caagttgtgt agttaagatg aaacttccag atgaaaatta tatatggaat
     3601 aaagttacct tgactactat ttttgatgaa gatggtaatc ctgtaagagc aattggtata
     3661 caggaagata taagcaaaca aaaggaagca gagataagat ataatcaaga aggtaagtat
     3721 cattcagcta tattgtcgga agcaatgata acctatgaaa taaatgtatc aaaagatact
     3781 tttatcaaag gaaatgaaag ttggcaaaaa ccatttggaa ttaagcctga caataattat
     3841 tctgaaatgg taaaaactat atcaaacagt attattcaca aagacaataa agaggtgttt
     3901 ttaaatacat ttttgtgtaa aaatttatta tctgcatata acaaaaatat aactgaaata
     3961 aaattagaat atcgaagaat gaatcaagaa aataaaatga tttgggtcat ttgcacaatg
     4021 cacttaataa aagattctat tacgaatgat ataaaagcac ttgcttatgt taaagatatt
     4081 gacaagcaaa aaaatgaaga aattaaatta aaattaaaag ctgaaagaga tactcttaca
     4141 ggattatata ataaaggtac tacagaaaat ttgattaatg atttcttgaa ctctgacgaa
     4201 gcaagcttag gaatgcatgc atttttaatt gttgatatag ataattttaa atctgtaaat
     4261 gataatttag gacatgtatt tggagataaa gtattatttg aagtttctaa acacctaaaa
     4321 cctatattta gaaaagatga tatagtaggt cgaattggtg gagatgaatt tatagtattc
     4381 ttaaaaaata ttcagtcttt tgagaatacc aaaaagaaag cagaaaaaat atgcgatata
     4441 tttagaaatt cttatacgga aaataaaaag acatataaaa tatctggaac agttggaatt
     4501 tcatttgtac cagaacatgg aactgtattt gaagaacttt ataaaaaagc agacatagca
     4561 ctttattatg caaagagcaa aggaaaagat aagtatgcac tttataataa ttgtatctac
     4621 caaaatgaac ttgaaaataa tataagtgat aataatataa aattagcaaa taaatgtgag
     4681 aaatccaaac cagtacttaa agaaattctt gattctattg ataattatat ttatatagtg
     4741 aatccaaata cttatgaact tctttttgtc aataataagg tgtctcgttt ggatttagag
     4801 ataaaattag gagaaacctg ttataaatca ctattaaaat ctgagagacc atgtaataat
     4861 tgccctctta acggattaga agaaaataag cattcaaaat tcaaaataga gacaaataca
     4921 tttggtaaaa atatcagtat agcaagttgg attaattgca tagatggaga atacaactgt
     4981 cttatagagt gtatagattc ttctttatac aaaaataaat aaaaaaggac tatgaagata
     5041 tgttaaatag tataactttg atacttggta taatatttac aatttactat ttttatctca
     5101 aaatagtttt tggaggaatt tcatttagtg agattttctt agttgtagga attgtactaa
     5161 ttatctatca aatatttaag aaaaaaatta aagaaaagaa ggttttttat agggtgttaa
     5221 aaataataat cagcattttt ttaatagtat ttgtagttac tgagagttta attatttttt
     5281 atcctaaaaa tagtttagaa tcaaaaagtg attatctctt aattttagga gcttctgtaa
     5341 aaaagactac accaagtaca accctgaagg gtaggttaga cactgcattg aaatacctaa
     5401 aaattaatga taattgttat gtggtagtat ctggtggtaa aggaagtgga gaaaaaataa
     5461 ctgaggcaaa agctatgaaa gattatctta taaaaaatag tatagataaa aataggataa
     5521 tagaagagga taaatccacg aatacttatg agaattttaa atattcaaaa ttgaagatag
     5581 aagaacatag tcaaagaaaa ctaagtgatt taaaagtaaa aatagtaaca acagattttc
     5641 atgttctcag aagtaaaata ttagcatata gaaatggata taaaaatacg tctttttatg
     5701 ctagtaaatc aaaactttca tttgtaccaa cttactatac aagagaattt tttgcattat
     5761 ggaaaacaat catttttgat agataataaa taaaaagact agatataaac tcatatctag
     5821 tcttttctat tcccttaatt ctaatccatt ttccaacttt acttgttgta gaaatttcaa
     5881 cattgggatt tacaagtaat tgcttataag aagttttata ttttacgatt ccaaaatata
     5941 attttccttc ataatccatt ataaatccga atgatcttac tcttggttta ttatcttcaa
     6001 ctgtagcaaa atagaatgtt gagttttctt ttaaaaaatt cattattttt tccatgatta
     6061 aatcctcttt gtatcttata ctaatataat gaatataaat aaaatattta taatactaat
     6121 ataaattgat ataaaaacaa ttacaatatt atattgatta ttcaaaatta tatataaaat
     6181 tcagaaaaac atttcagttt acataataaa aataaagtaa actaaaatta ttgattttag
     6241 attaaagtgc cagattaagt atttaagtag attttaagct tgatttgatt agaaaggtac
     6301 aaactatcac cgaaaaaaat taaacccatt taaaaagcaa tataaaagct tgttattttc
     6361 gagaaaaata gaccctctaa actcaattag ataaaaataa aatacaaaaa attatatctc
     6421 tgaatatctc aagtaattaa gtttaaattt tacgatattt tgtttgatga tttataatat
     6481 aaaagcatat tcttaatctt ctcaaattaa aaagagtaaa aaataacaaa aaaatcactg
     6541 acatttcact gacaaattgt aattttgtta ttataaatac attgaaaata cttgattatt
     6601 gaaaaatggc ataaaataat accttttatg ccatttacta ttgtttgata gtttttaact
     6661 tatgacataa gatatttttt aacatataca ataaaatata aaaaaattga tattgcaaat
     6721 aaaaaaaata catattagat gttatagaaa aaaagcgcac ttataggctt aaaatgtcac
     6781 ataaaagagt ttcaaagaat aatatattat gaacacaaat tttgtaatca caaaggagtg
     6841 ataatatggc aacagtatca ggacaaatag tttttgatag aaatagaagt gctaccattg
     6901 attctgggga ctcaggaata gccaatatac cagttgtatt acaagatata gcaactggag
     6961 ttagacttgt ggttctcaca gatgcatcag gaaattatgt ttttactaat gttccaaatg
     7021 gtaactatag aattgtagaa gcttttggaa ctacaggagg agtaccaaca ccagggaatt
     7081 ttgcaacagc gatagtaggt ccagttccaa ttgcagaaat gccacctatt agttctgtga
     7141 caaatcctcc tattggttca acaaacttag atggtacaac acctaacaca ctattaataa
     7201 cagtagcagg ttcagatata ataaatcaaa atatattaaa tgggcctgtg atttatacac
     7261 ctatagagaa tatattggat tcttgcgtgt ctgtatctaa tataaacctt attacagatg
     7321 cagataacgg aacattcggt ttttttccac caggtacacc aagtaataca ggacctgaaa
     7381 tagaacctta cccaaatgtg acgccagatt ttacgtatgt tgtaccagac ccaactaagt
     7441 ttacaccttt agatggagaa tatacagtac aaaatattat gacaaatgca atgagtaatg
     7501 ttataggagc ttggtggcgt attgctgacc atactacagg aaatgaaaca ggaagaatga
     7561 tggtggtaaa tggttttaat cctggagctg tattttttaa atcaacagta caagttacac
     7621 ctaacactaa ttatttattt agcacttgga ttttaaacct ttttaaggta acaggatatc
     7681 caccaccaca gctaggtgta agaatatttg accaaaataa taatatacta tatcaagcaa
     7741 cattaggagc tcaaattcca gttaatacaa acgcaccaga atggaaagaa gtaggtactg
     7801 tgattaattc tcaaaataat aatcaactta cagtagaatt ttttagtgaa ggagaaagcg
     7861 ccattggaaa tgactatgct atagatgatg tttctttaag agaaatagct gttccaacct
     7921 ttactccaat aaaaacaagt aacacagaaa ctgctaatgt aggtgatata atcacatata
     7981 cagttacatt agataatact tgtgatagcc ctttaactaa tgtatttttt agggatttaa
     8041 ttccaaatgg tttagagttt atacctggaa ctgtaacggt tgatggagta tctgtaccag
     8101 gagtagaccc taatacaggt tttccacttc cagatattgg aggaggaata ggggttgaag
     8161 taacttttga tgttgtggtt gaaagtattc ctaacccaaa tcctactaat aatatagcta
     8221 ctatagatta ctcctataca cctgtagaag gcggtatacc aaatgatttt tcagtagatt
     8281 ctaatccagt tcctgttgaa gtaatctcag cagatgttga agttacaaaa ttatctgaac
     8341 ctactatagt gaatccagga gaggaattaa tatacactat aaaagtagta aataatggac
     8401 cattcccatc tgagaatgtg gttttaacag atgatgtacc agcttctatt ataaatccag
     8461 agtattcatt agatggagga gtaacttttc aaccatggac tggaagttta aatataggga
     8521 cattagaagt aggagagaca agagttatta taattagggg tattgtaaat ccatcaacag
     8581 taggaattat aacaaataca gctgtagtat cttcaactac agcagaccca aaccttaata
     8641 ataatacatc tacaatagag acagaagtga atttattagc agatatttta gtaatgaaaa
     8701 cagcagaacc taattcagct gttccaggta ctttattgag atatactatt caagtagaaa
     8761 acttaggacc agcaaatgct gaaaatgtaa tattaaatga tgatattcca gcctctatta
     8821 taaatccaga atattcattg gatggaggag caagttttca accatggaat ggaagtctta
     8881 atataggaac tttaaactct ggaatttcat taacagtttt aattcaagga acagttagtt
     8941 taaattcttc agaatatata gcaaatacag caacagtttc ttcaactaca cctgacccag
     9001 atttaagcaa caatatatct accattatta caccagttaa cccacaagca ggtatttcta
     9061 ttataaaagt agctgacgag gatgtagctg ttccaggaga agaatttgta tatacaattg
     9121 aaatttttaa tgaaggacca tctaatgcta cgaatgtagt tttaacagat gatataccag
     9181 atgttatttt aaatccagaa tattcattgg atggaggagc aactttccaa ccatggaatg
     9241 gaagtcttaa tataggtaca gttgcaccag ggcaattaat aagaattatc ataaagggat
     9301 tagtaagttc aactgctaca ggagatatta ctaatacagc agaagtaagt gcagatgtac
     9361 cagaaccagt tacggattca tcaacagtga caactcctat agttccatca gcagatatag
     9421 aagtaataaa aactagcaat atggatacag cagttccagg agaaactttt agttatacta
     9481 taacagttat aaatttagga ccatctgctg cacaaagtat cgttttgaca gatgatatac
     9541 cagatgttat tttaaatcca gagtattcat tggatggagg agtaactttc caaccatgga
     9601 atggaaatct tagtataggc actttggatg ctggagaaat aagaagtatt ataataagag
     9661 gaacagtgag ccagacagca gtaggtacaa ttataaatac agcaacaata agttcaccta
     9721 cacctgaccc aaatccagat aataatactt caacagatga aacagatata agtccattag
     9781 cagatatttc tgtgataaaa ggcaacgaac cagttgctat accaggggga agatttattt
     9841 atggaattga aattgcaaat gcaggaccat cttttgcaga gaacgtaact ttaacagata
     9901 atataccagc ttctatttta aatccagagt actcaataga taatggagtg actttccaac
     9961 catggaatgg aagccttaat ataggtactt tagatgctgg agagataaga aatatcataa
    10021 taaggggaa
//
LOCUS       DAMKPG010000117         9743 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMKPG010000117 DAMKPG010000000
VERSION     DAMKPG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.122"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            88..762
                     /locus_tag="PC621_003185"
     CDS             88..762
                     /locus_tag="PC621_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110321.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            786..1790
                     /locus_tag="PC621_003186"
     CDS             786..1790
                     /locus_tag="PC621_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110322.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            1902..3131
                     /locus_tag="PC621_003187"
     CDS             1902..3131
                     /locus_tag="PC621_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF4110323.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            3139..3813
                     /locus_tag="PC621_003188"
     CDS             3139..3813
                     /locus_tag="PC621_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110324.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            3816..5048
                     /locus_tag="PC621_003189"
     CDS             3816..5048
                     /locus_tag="PC621_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110325.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(5334..6599)
                     /locus_tag="PC621_003190"
     CDS             complement(5334..6599)
                     /locus_tag="PC621_003190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF4110326.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(6652..7800)
                     /locus_tag="PC621_003191"
     CDS             complement(6652..7800)
                     /locus_tag="PC621_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF4110327.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(7891..8436)
                     /locus_tag="PC621_003192"
     CDS             complement(7891..8436)
                     /locus_tag="PC621_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF4110328.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(8659..9603)
                     /locus_tag="PC621_003193"
     CDS             complement(8659..9603)
                     /locus_tag="PC621_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF4110329.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         3468 a         1499 c         1308 g         3468 t
ORIGIN      
        1 tattttaggt aataaggtag aaatatttta atttatcaag tagaatatag ttatgttaat
       61 ataactattt tgaattagga ggtaagaatg tttaaaataa tggtagttga agatgacgtc
      121 tcattgaaaa atatcatagc aaagtgtctt actaaatggg gacatgatgt tcatcaaata
      181 gaaaatcttg aaaatataat agaagagttc aaaaactata acccagaatt agttttattg
      241 gacataaacc ttccatttta tgatggtttt cattggtgta atgaaataag aaaaatttca
      301 aaagttccta taatttttat ttcttcaaga aattctaata tggatgtaat aatgggtgta
      361 aatttaggtg cagatgatta tatacaaaaa cctttttctg tagatgtctt agtagctaaa
      421 gttaatgccc ttttaagaag aacttataat tttgttgata ataactcaaa tcagattatt
      481 cacaatggtg ttactttaga tttatcaact gcaacaataa actatgaaga taatactatt
      541 gagctaacaa aaaatgaaat caaaattctt catgagctta tgaaatataa ggggcagata
      601 gtaagtagga ataaactcat gaaaaaatta tgggataatg attggtttgt agatgataat
      661 acattaactg tcaatgtaaa tagaatacgc tcaaaattaa atgaaattgg tttagaagat
      721 tttatagaaa caaaaagagg attgggttat ataatcagtt aaaacaattc taagtaggtg
      781 aatttatgag ctttaaagat tttctcaagg ataaactagg ttttttagta tacaatttta
      841 ctttcttaat ttttacagta tcagttatta tattttctcc tgtagatgtt tttctgactg
      901 atactatttt atatatatta gttgtaaatg ttgtgttttt atttttatat ctatttatta
      961 gctacatcaa gaagaacaaa tttttagata agataaaaaa tgatacattc aaaattaata
     1021 tgaatgatat agaattagct agatgtaaaa atgaagaaaa aatatacttg aacattatta
     1081 aaaatcatca atatcaatgt aataacataa taaataataa tatcgagaaa tttgaagaaa
     1141 acaaagagat tttaaatatg tgggtacacg atgtaaagat acctatagct ataataaaac
     1201 tcattcttga acaaaatgaa aatttaatta atgaaaaaat ttcagatgac atagatagtg
     1261 aaatatttag aattgaaaac tccgttgaaa aaattttata tttatcaaga ctcgaagatt
     1321 ttcataagga ttttttaatt caagaggtaa atattgaaaa aatcattaga aatattataa
     1381 aaaaatactc aaaatatttt atttcaaaaa aaattatttt agacttaaat aatttgaatt
     1441 ttaaggtttt atctgatgaa aaatggctat acttcatatt cgagcaatta ttgagtaatt
     1501 ccctaaaata ctcctcatta ggagataata tatctatcta ttgcaaaaga accaaaaatt
     1561 atttacaatt aagagtggaa gatactggtt gtggtataaa aaaggaagat ttaaatcgaa
     1621 tattcaataa gggatttaca ggtaataatg gaagaaacaa ttctaaatct acaggtcttg
     1681 ggctttattt agtaaaagaa ctttgtgata agcttggtca taaaattgat gtagattcag
     1741 agtacaacca atatacaaaa tttactatta cattcaaatg tgatttatag tatcttatta
     1801 ttgatagttt attttcatta acttatattc aacttcttat agtaattcta tatgtacatc
     1861 ccagtatata attaagtagt ttttataaaa aggaggaaga catgtctttt ctaaaaaaat
     1921 ttaaaaataa aagatttaga aataacagtt ttaaaagtaa caactttaga aatagcagtt
     1981 ttggaagtag tggcttcaag tctaaaaata aaggttttaa aaacaatccc tttaaaaata
     2041 agagattcaa aatatatgca ggaatagcag taatcatact tttaattcta ggagttcttg
     2101 cttatgttga ttcgaaaaac actgaattac aagctaatga aaattttatc gaaacatata
     2161 ctattcctga aaatgaaaaa atatttataa atgggatggt tgttcctaag caaactaagg
     2221 acttcaatat atctggagat tatgagttaa gtgatgttaa tgttacaaat ggtcaaaaag
     2281 taaatcaagg agaccttctt tttactgcta aaaatccaac tataatagca gaaatagata
     2341 gtttaaaatc acaacttagt caatacaaaa aacaaaaaat atcactttct gatattgctg
     2401 aaaataaaga tgctattgct tctataaatg cacagataac tgctttaaac tctcagatag
     2461 cttctctgga taaaaaagct tatgacagag ttactgctcc ttttgatggc acagtatact
     2521 tgaatgacca aactggaaat ccagaccaac cagtttcatt tatgactatt caaggtctag
     2581 agttttatat gaaaggacaa gcaagtgaac aagatttacc taaaatgaag attgaccaaa
     2641 tggtcaatat actagttttt tcaactgacc aaaagcttac tggtagaata agttttatat
     2701 ctgataaacc atctactcca aatactgaaa tgggagctca gcagaataca ctatcttatt
     2761 atgatataaa catagcattt gataatcaag aaggattagt aaatggattc catcttcaag
     2821 catcacttga agtagctaat tcaagcttta aaataccagc atcttgtgta cttaaagaca
     2881 acaaacactc atatgtattt aaggatttag atggtatatt gaaaaagcaa attgtagatg
     2941 tagcaagtca aaatgatgat tttgctgtag ttagaggcgg tcttgaacaa ggcgatataa
     3001 taataaaaca ccctactaaa gaaatgaaag aaggagaccc agttcaaggc gatggcgtaa
     3061 ctgctggttc taataatgga gatactactc caaacaaaaa agtagaagaa gttactatgg
     3121 atgtgaatta atttacatat gttaattaaa ttagaaaata ttcaaaaata ttacaaagta
     3181 gggaaagatg aattacatgt tttaaaatcc ttaaatttag aaattgaatc tggagaattt
     3241 gtcatgataa tgggtaagtc tggtagtggt aaaactactt tacttaatat cttaggtttt
     3301 ctagatgtat ttgatgaagg aagatatata tttgatggaa cagatgtaac taatttgagt
     3361 gaaaatgaac gttctgtttt tagaaatatt aatattggat ttgttttcca acaatttaat
     3421 ttaatagaaa ctctaaatgt atatcaaaat gtagagttgc ctctaattta caataaagca
     3481 ctaaaaaaat ctaatagaga agaaattgtt aaagataaat taagttctgt tgggcttttg
     3541 gacaagctta agcagaaacc acttcaatta tctggtggtc aacaacagcg tgtagccatt
     3601 gcacgttgcc tagtaaatga ccctcagata atctttgctg atgaaccgac tggggcatta
     3661 gatagtgaaa ctagcagaga aattatggag cttttaacaa ggctcaacaa acaaggtaag
     3721 actataataa tggttacaca tgatcaagat ttaactaagt atgcaactaa ggttatacga
     3781 cttaaagatg gtgtgtttac ttcggaggtg taatcatgag tttattaaaa aattctttgg
     3841 ccaatttaaa aggtcacaag cttcgtgtat ttgtagcttt actctggatt ataataggaa
     3901 taacttcagt aatacttgtt agttctatag gtaatggatt tcaaaaagaa attaagaaat
     3961 ctgtaaacaa tgtaaaccct aataaaacta ctatttcgtt tgaatcagcc gataacacag
     4021 gtcttactga tgatatgagt atttttttaa aaccctttaa tgcaaaagat ttagaagaat
     4081 tatcatttgt tgaaggagta gaaagaatag ctccttcaag agatgggttt aaccttgatt
     4141 cagtttactc ttcacaagca tcttttgata aaaaaactac gtatgttgat gttggaccag
     4201 taaaaaaaga ttctaaaata aatttaattt gtggtagaga tttttcttta gatgatgaaa
     4261 aaagaaaagt aattttactt acattacaaa gtacaagtga aatttttgaa aatccagaag
     4321 atgccttagg tcatggtatc aacataaatg gaactatatt tgaaataata ggggtattag
     4381 atgattctca gcaaaaccaa gctggaggat tctttggagg ttaccaagat atgcaattta
     4441 ctacttccct agtacctaaa aaggcttttg acactttaat gagtcaaaat tcttattcaa
     4501 atgaaattta ccagttagac ttagtttctt ctaaaggcta taatgtaaat gaagttgcaa
     4561 acaatgttat agctaaatta tatgagatgc accctggtat caatggttct tatactacac
     4621 cagacccaac tgaacagaca gcttatcttg aaagcattaa ttccaatgta aacaaatatg
     4681 tatctataat aactgtagtt gcaatgtttg ttggtggtat tggtgtaatg aatataatgt
     4741 atgtatctgt tatggaaaga caaagagaaa ttggtataag acgtgctatt ggagcaaaac
     4801 ctaggtctat attatttcaa tttttagttg aagctgtttt tataacagta tgtggtggaa
     4861 ttttaggaac tatagttgga tttgctgcta ctaactatgt ttctaaatat ataggttttg
     4921 aagctatacc tagcttaaat agcttatttt atgcaattgt agctactata ttgactggag
     4981 ttgtatttgg tctgattcca gcctttaaag cttctaaact agaccctata aaggcaattt
     5041 ataagtagaa attgtataaa gattttcgat ttttagatta gtttatataa aacatactta
     5101 aatatataac gcactattat atatgataga ttaggaactt aatctttcca aatataatag
     5161 tgcgttcttt cataaaaagg attctcttcc ttactatatc taaattacca agatgttatg
     5221 ttctaattgt tcaaactaat acttccaatt ttgattaatc aatctcattc tgctgatata
     5281 caatctttta ctttcaatca ttgtacactc cgccttaaaa aaataatctt aaattaggcg
     5341 ttatatacaa tctccccatt acatattgta taattaacct ttccatataa agatttacct
     5401 ttaaacggtg aattatcagc tttagaaaca aagctttcaa ctgtccattc ttcttctgga
     5461 ttgaatataa caatgtctgc tacagcccct tcttctataa atccaatatt taaattgtac
     5521 aactttgcag gatttataga cattttttct attaactgca tcatactaag atgattttta
     5581 tgcaccaaat ttgttattcc aagagctaaa gaagtttcaa gcccaataat tccacttgga
     5641 gcttttataa actctctatc tttttcttct ttactatgag gtgcatggtc cgttgctatt
     5701 atttcaatag taccatcatt taatccctct ataatcttat ctctgtccca tttacttctt
     5761 agaggtggat tcatttttgc atttgttcca aactccaata catcttcctc agtcaatgta
     5821 aagtgatgtg gtgaagcttc tgcaactaca tttgcaccta agtattttgc aaacctaacc
     5881 atatcaacag atacacctga acttatatgt tgtatatcaa ctttagcacc agtctttagt
     5941 gccaacatac aatctctggc taccataaca tcttctgcta cacttgatgc accttttaat
     6001 cctagttctg aagctacctt tcctgcattt acacctggat ttccaactaa agaagggtct
     6061 tcttcatgaa aactaagtgg tacatccaac tcttttgcct taatcattgc ctccataatt
     6121 agattgctat ccattaaagg taatccatca tcagtaaacc ctggtacacc agctttttta
     6181 agagcttcca tatcaactaa ctctttacct tcaaaacctt tagttatagc tgctgcttgt
     6241 aaaacattta tacatgcatt ttttgacttt tctttaacat aattaaaagt atcttcatta
     6301 tctacaatag gattagtatt tgccatacat ataacagttg taaatccacc tctagcagca
     6361 gatttagctc cactttctat atcttcttta tatgtaaacc cagggtctct aaagtgtaca
     6421 tgtacatcca ctaaacctgg agcaactaca ttaccactag catctattat tttttcatat
     6481 tcatcacttt catcaaactt gcctatttta taaattttat tttcttttat aattaagtca
     6541 atattctcat ctctttgact tttagggtct attactctac catttttaat taaaatcata
     6601 ttatttctcc tttatatttt taatttatat agactcatat aatattatac attattttac
     6661 atacttaaat acaaaattac gtttgacatc aatagccttt ttaggacaaa gttcatggca
     6721 acagtaacat cttatacatt tacttaagtc tataatagca cctttagtcc ctatctctat
     6781 agtggatgca ggacaagctt ccttgcattt tccacatttt atgcaatctt gaaatcttac
     6841 cacaggcttt ggtgttataa gctttgaaat aggttcattt aaaaattttg gtagctttac
     6901 ggaaaacaat cttaaatcct tacttacatt tggaatcttg taatttttta taattaaatc
     6961 ttttatatct tcacctaaaa tttcaatatc tgaaaaatct tcttgtatat agcctctctt
     7021 aatagcacct ctaattgtag caactctatt tggaagtaaa cttataatct tacatgcaac
     7081 aacatctata gcatatggat tttgtgatgc taataaaact cctatttctc gagtagtacc
     7141 agctgaaggt ccatcacctt ccatacctac tataccatcc ataattgtca atgttggttt
     7201 tatataatca caaatatcta aaagtacatt ttcgcaaaac aactcttctg taggaaatct
     7261 ataatgtatc tctgcttttt tcaatcctgc tatgcatcca tataagtttt ttgtaccacc
     7321 agtaaaggtt gccattatat gagtctttaa tttacaaaga tttattatat aatctgaatc
     7381 tactattggt ttaataatat ccatatattt tagaacatgt gcttttttac tatgtatctt
     7441 aacatctgaa acatcataat ttaacttaat gcccagttca tttgctactt cttcaattcc
     7501 agatgcctta taaatgctct taagagaatt ttgattatat ggaccaccag ggctatctgc
     7561 tattgtaata tcacaattta actctaaaag tttttcacat acaactttta caaccatagg
     7621 atgtgtagtt gtagcttctt caggcttttt tttcattaat aaatttggtt ttataagtac
     7681 tttgcttcct tttttaacaa acttatctat accaccaata tcctctaaat ttttttcaat
     7741 agataacttt acttcttcat aattgtaagt tttacaactc ctaacagata ccgacttcat
     7801 attatctcca tccttctatc acaacagttt atttgtataa tttaaaaaag gagaaaccaa
     7861 agtttctcct tttaatctat gttttaatta ttattttcca aaatgtctgt ttaataatcc
     7921 taagaatgct ttaccatgtc tagcttcgtc tttacacatt tcatgtactg tatcatgtat
     7981 agcatctaat cctaattctt tagctctctt agctaatttt aattttccat cagttgcacc
     8041 atactcagca tcaactctaa ctcttaagtt ttcttttgta tctgcaacta caacttctcc
     8101 aagaagttct gcaaatttag cagcatgttc agcctcttca aaagctattc tcttatatgc
     8161 ttcagctact tctggataac cttctctatc agcttgtcta ctcattgcta aatacattcc
     8221 tacttctgta cactcaccag taaagttagc tcttaatcct tcgattattt ctgcatctac
     8281 accttgagct actcctattc tatgttcatc agcccaaacc atttcgcctt tcatttcttc
     8341 aaatttatca gctccaactt tacatactgg acattgtgct ggtgcagcat ctccttcatg
     8401 tatatatcca catactgtac aaacaaattt tttcataatt aatttcctcc caattttata
     8461 taattaaatt tttattttta agtttaataa cttgctacaa gaatatatat accccagact
     8521 ttatagctta aacactaaaa ataaaattta tttcctaatt aattttatca ctttgaaaca
     8581 ccagtatcaa ccaagttttg atgtcaaatt tctacataat acataactta tttgattaat
     8641 taggaaatag ttgtcatatt aaatactttc taatgaagac tcctctgaat ctttaagttt
     8701 cacttttttg ctctttaaaa aagataattt agtttctgca gttatgattg caacaaatat
     8761 caagatacta ccaataatca attttgctgt aataagttcc tttagaatta ttatagaaaa
     8821 tattgtacca aatacagcct ctgtggataa tattatagct gttctagttc catcaactct
     8881 tttttgacat atagtctgaa ataaaaatcc aattgtagta ctaaatacag ctagatacat
     8941 agttcccata taaccagata attcagcttc catctttaat tgacctgcaa atgtttgaac
     9001 tacaacagac attaaaaacg ctactgtaaa ttgaatagct gtcaatgcca ttgggttgtt
     9061 atcttttgca aactcacttg taaagaatat atgaaatgca aatccgaatg aacatataag
     9121 tgttagaaaa tctccaaaat taatagagaa atcagcttct aaagaaagta tcccaattcc
     9181 tataagagct accaaactac ttattattcc tattttatca agttttcttc tataaagtat
     9241 aaatcctatg aacggaacta ttacaacatt agctgctgtt ataaatgcat ttttagatgg
     9301 tgtagtgtac ataagtccaa tagtttgtac tgtgaatgct agaaataaaa atattcctaa
     9361 aatccctcct gcttttaata attttttacc catattggca cgaatctgtt taaaaaacag
     9421 taaattcata attatagctg ccaagaaaaa tcttaaagtt atgatttgta gaggtgtaag
     9481 cccaccgtct aatgcaaatt gggttgctac aaagccactt ccccatataa ttgctataaa
     9541 tattagtcct atttctccaa agtatttttt caataaagcc ccctcctttt tatttttttg
     9601 caaaattata aaccccctaa acagcaagag agtttttcta taagcattac tataaattaa
     9661 tatagtaaat tttttcagta ttttaaatct aaatttacat acattttgag ctcaaattta
     9721 tatccactat ttaacagtaa tat
//
LOCUS       DAMKPG010000118         9707 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMKPG010000118 DAMKPG010000000
VERSION     DAMKPG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.123"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            494..1395
                     /locus_tag="PC621_003194"
                     /pseudo
     CDS             494..1395
                     /locus_tag="PC621_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1382..2137
                     /locus_tag="PC621_003195"
     CDS             1382..2137
                     /locus_tag="PC621_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4110330.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            2268..3290
                     /locus_tag="PC621_003196"
     CDS             2268..3290
                     /locus_tag="PC621_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4110331.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(3454..4011)
                     /locus_tag="PC621_003197"
     CDS             complement(3454..4011)
                     /locus_tag="PC621_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF4110332.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(4017..5093)
                     /locus_tag="PC621_003198"
     CDS             complement(4017..5093)
                     /locus_tag="PC621_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF4110333.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(5094..5777)
                     /gene="modB"
                     /locus_tag="PC621_003199"
     CDS             complement(5094..5777)
                     /gene="modB"
                     /locus_tag="PC621_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF4110334.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(5926..6738)
                     /gene="modA"
                     /locus_tag="PC621_003200"
     CDS             complement(5926..6738)
                     /gene="modA"
                     /locus_tag="PC621_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110335.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            6931..7860
                     /locus_tag="PC621_003201"
     CDS             6931..7860
                     /locus_tag="PC621_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110336.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(8021..8470)
                     /locus_tag="PC621_003202"
     CDS             complement(8021..8470)
                     /locus_tag="PC621_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110337.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(8673..9653)
                     /locus_tag="PC621_003203"
     CDS             complement(8673..9653)
                     /locus_tag="PC621_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110338.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3348 a         1545 c         1209 g         3605 t
ORIGIN      
        1 tatattctac atgtaaaaat ttttttattt aaactcaatt ttctgataac ttgataattt
       61 ttcaaaattt atagttgact tttatttttt actgtattaa actaattact atacaaaaca
      121 gaaaacaaaa ttttcaaaat tattaaatta aaaggagagg taaatatgaa gactttatta
      181 aacaaacaag tacgattttt tagtagccaa tttagttagg gtttgtacct aagttaagac
      241 aaattgtcat aacatagata caagcccctt aggattatga tttttaagga ttgtatctat
      301 gttggctata gtaattgttg ttaaagtttt tattttatca tcataatata gagccacatg
      361 gattatactg tgtggcttat tcgaattttt gccatacagt tatatatact gtgtggtttt
      421 tttatatcaa aatcattttt taaaaccaaa tttaaaaaac catttaaaat attttcaaag
      481 gggtgtttta tttatgtcag gtattatatc tgtaatgacg caaagtttaa ttttatcgat
      541 tatggcactg ggagtttata taacttacaa aatcctagat tttccagaca tgtctgctga
      601 tggtagctat acaatgggtg cttctattgt agcattttct cttactaatg gtatttctcc
      661 tgtagtagca actttaatgg ctatcttatg tggttgtact gctggtcttg ttacagggat
      721 tcttcacatt aagtttaaga tatctaactt actttctggt attttagtta tgggaatgct
      781 atactctatc aatttaagaa taatgggaaa atcaaatatt cctctattta gctttaaaca
      841 cttattcaat ggggaaatat ctcctatagt tttagcctta gcttttgtat tcatctgtaa
      901 aatcctttta gatttattcc taaaaacagg tcttggatac actttaaaag gtgttggtga
      961 taactctcaa atgattaaat ctctgggtat aaatatcggt tctataaaaa tactaggact
     1021 tatgatttca aacggactta tagctttatc tggtagtctt atggctcagt tcttgggatt
     1081 ctcagatgta aatatgggta taggaacttt agttcttgga attgcctcta tcataattgg
     1141 tattacacta tttaagaaat ttacttttat caaagatact actgcaataa ttgttggctc
     1201 ttttatatac caattcacta tatactttgc aatgagttta ggaatgttat caactgactt
     1261 aaaactaata actgccatag ttataatagc tttcctagct actggtaact taaatatttc
     1321 actaaaaaaa acaaatgcaa agctagtacc aaaaataaat cagaaaaaag aggtgttgta
     1381 gatgttacaa attaaaaatc tttcaaagag tttccctaat ccatatggtg aacctaatac
     1441 tatctttgaa aatttatcaa tcgatataga agatggtgaa tttgtaagta tcataggtag
     1501 taatggaact ggtaaatcta ctttattaaa tataatatcc gggcttataa aagaatcttc
     1561 tggtcaagta ttattaaata aacataactt gtctaattta gctgaacaca gaagaactca
     1621 aatagtaagt agagtttttc aagacccaag tcttggaact tgcccttcta tgactgttag
     1681 agaaaacttg tctttagctc ttaacaaagg aaaactatta aatctaagaa aatgtcttcg
     1741 ttacaaaaga gatttcttag aaaatttatt ggaaggtgta tctcttgatt taaaaaaata
     1801 ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga caatcattat cactaattat
     1861 gtcgtgctta acaagtccat cagttttatt acttgatgaa catacagcag cacttgaccc
     1921 aaaaacatca aatgaagtaa ttgaattgac taataagatt gtaagggaaa aaaatataac
     1981 tacacttatg gttactcaca acttaaaaca cgctcttcat tatggtgata gattgataat
     2041 gcttcataag ggtgaagttg tattagatgt aaaaggtaaa gaaaaagaac aattaacagt
     2101 tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat aatatatcta ttgtattaaa
     2161 ctcggcttta aaataaagtt aaaattacaa taattatggt tactttgtaa taattcatat
     2221 gaatatatga acatatataa taataaattt ttgaggggga tataaaaatg attaataaaa
     2281 aaagattggc aagtttgatt ttagctggtg ctcttagtat atctatgctt acagggtgtt
     2341 cacaaggagg agactctggt aattcaaaac aagaaagtaa ttctaaaggt aaagaagtta
     2401 aaaaaattgg tataactcaa ctggttgaac atccagcatt agatgccact aggataggtt
     2461 ttgtaaaagc attagaaaag aacggattta aagatggaga aaatattgat atagacttcc
     2521 aaaatgctca aaatgacatg cctactacac aaagtattgc gagtaaattt gcatctgata
     2581 agaaggactt aatatttgct atatctactc catcagctca ggcagcattt aatgctacta
     2641 aagatatacc tatacttatt actgctgtga gtgaccctgt tgctgctgga ttagttaaaa
     2701 ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga ttttgtttct gtagacaaag
     2761 gtttagaact tcttaaaata tttgctccta aggcaaaaac tataggagtt atgtacaata
     2821 ctagtgaagt aaattccaaa gttcaagttg atgctttaaa agaatatgca tctaaaaatg
     2881 gatttaaagt tgttgaaaaa ggtataacta cttcaaatga agttaaccaa ggtatttcta
     2941 gtttagttgg caaaatagac gtattatatg ttcctacaga taacttagtg gcttcttcta
     3001 tgcctatagt ctctaaaata gctactgaga ataaaatacc tgttatagca gctgaatctg
     3061 ggccagttga aaaaggtgct cttgcttgtc aaggtatcaa ctacgaaaaa ctaggttaca
     3121 aaacaggtga aatggcagtt aaaattttaa atggcgaatc agtatctgat atgcctgtgg
     3181 ctacatcaga tgatacagat atcattgtta atgaagatat attaaaagct ttaggtatgg
     3241 aaaaaccttc taatgaaaat atttcttatg taaagactaa acaagaatag aaagatattt
     3301 tctatatacg attaatatgc ctgtttctat aaattaattt atagaaacag gcatatattt
     3361 ttattttaat gtattatcta tttaaagatt atatgtaaat gctcttaata tgattgtata
     3421 aattttactt gtataaactt tatacaatca tatttattct tctattttct ttattactct
     3481 acatggattt ccaacagcta cagtatttgg tggtatatct tttgtgacta cacttcctgc
     3541 acctattact acattatcac ctatagtaat tcctggtgtt atgattactc ccccacctat
     3601 ccatacatta tcacctattt ttacaggaga accatactct atgcctgaat ttctaagttg
     3661 tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt ggagctaaca ttacattgtc
     3721 acctatttca attttacata catctaaaaa aatacaatca taatttgcaa agaaattttc
     3781 gcctacatgt atgttatatc cataatcaca cctaatgttt tgttctacat ttatttgctt
     3841 gccaactgaa ccaaatagtt gtcttaaaat atcttctctt ttttcatatt catcctctaa
     3901 agtattatta aacaatctag ttaatttttt acaatactct ctttctttta ctaacaattc
     3961 atcatttgca tagtatcctt ttccagacaa cattttttct ttttcagtca tatagtttac
     4021 tcctttttgt tataattgta tctaaagcaa aataaatatt cctgtggaaa atctaaatat
     4081 attctgtcac ctttcttaaa tctcatttta cttttttcca attctatttg aataggtaca
     4141 cattcgttag ataaatcagt ttttctaaca tatatagtat atgtaaaagg attttctaca
     4201 atgttaatca attcgaaaca taacttttca tttaaatctt cattagaatc caagacctta
     4261 atatgatgct ccctgattcc cacatattct atattatcac ctatttctct atttaatgtc
     4321 aactcgcatc cccagtctgt agcataaata gtatttttat ccagtctatt taatttggat
     4381 atatttttac accctgttat cttagcttct ataaggtact ttggagattc aaagagctct
     4441 tttttaggtc tctttggaag accttctcct ttgttgtaca ctataatatc atcacaaact
     4501 cggtaagctt cttcaatatc atgagtaaca aatagtatat ctcccttgta atcttttaac
     4561 atattcataa gttctttttc catattactt cttaaatgat ggtccagtgc tgagaaaggt
     4621 tcatctaaaa gtaatatatc tggagatgtt gccaatgctc tagctagtgc taccctttgt
     4681 tgttgaccac cagataattg ccaaggatat ctcccttcaa acccatttaa tttaagtata
     4741 tctaagtacc ttgctactat ttcctttttt tcactcttac ttagttttaa aagcccaagt
     4801 tctatatttt gacttacagt catatgtgga aacaaagcat agttttgaaa taaaaatcct
     4861 acctttctat cttgtgttga taaatttatc ttcttttcag aatcaaatag aactctatca
     4921 tttaatacaa ttttacctct tgttggtttc tcaagccctg ctatacattt taatgtcata
     4981 ctttttccag aaccagattc ccccaaaaac cccagagtac ctttttcttg tttaatttct
     5041 acctttagtt taaaagagga taaatctttt tcaatatcca cgtataagct caactatcca
     5101 cacctctttc ctataatttt ttgctgaaat tccgaccaat aatttagtaa taaaatcatt
     5161 gctatagata ttgctacaat aatcataacc cacatcatag ctttattcat atctccgcct
     5221 tcaacagcaa aaaatatagc tattggcata gtttgagttt ttccaggtat atttccagct
     5281 atcataagtg tagcaccaaa ttcgccaaga gctctggcaa acgacagcac caatccacca
     5341 attattccag gccatgctaa tggaactgca atcttaaaaa atactttcca ttcattaaga
     5401 cctaatgttc tagcagctga cagcatatta ttatcaatct gttcaaaagc tgaccttgaa
     5461 gttctataca tcataggaaa agacaccact actgctgaaa ttacagttgc tgtccaacta
     5521 aatattaaag tcctatcgaa atttaataaa aactttccta taaatccatt cttcccacat
     5581 agtaataata aaaagaaacc tacaactgtt ggtggcaaaa ttagtggaag tgtaaatagt
     5641 ccatctataa gacccttcca cttacctcta tagttagcca taatatatga cacaaatatt
     5701 cctattacaa aagttattat tgttgacaaa atagatgtct taagtgatat ccataagggt
     5761 gaccaatctg ttcccatctt tccctcctta attttaaatg gaaatagtga aactaataac
     5821 tctattaatt tcactatttc cattttatta taatacacaa ttattttcaa ttttcattta
     5881 tttatatata atttatactt aattacacat aatttattta taagtttatt ctactttttt
     5941 atatccaaat tcttcaaaaa tcttttgtcc tgactcactt aataaaaatt cttcaaattt
     6001 cttagcagca tctacgtttt tactagcttt aattactgaa actggatatg taataggtga
     6061 atgagttttt tcgtctgttt tttcaactac ttttatctta tcattattga cagtatcact
     6121 gaagtaaacg aatccaacat cagcatttcc tgattgcacc catgctaaaa cttcttttac
     6181 atcttttgca aatactagct tatcttttaa cttatctttt aaatttaaat tagttaacac
     6241 ctcatctgca tacttaccag ctggaacact ttcaacttca ccaacagcaa ttttcttcac
     6301 tttatctgta gtcaaatctt tcatacctga tacactactg tcagcagaag aaataagaac
     6361 taaatcattt tttactaaat ctttcatagt atcagaaact aataacttct cttcatccaa
     6421 agctttcatt tgtttttgac ctgctgaaat aaataaatca caaggtgctc cttgttctat
     6481 ttgttgttgt aatgagcctg atgctccata gttaactgta agttttacat ttgaatcaac
     6541 ttttttatat tcttcttcaa tctttgccat tgcttctttt aaacttgcag cagccgatat
     6601 atttaactct actgaatcac tacttttagt tgttttttct tgttttccat catctgtttt
     6661 gtcttcatta ctattgcacc caactgtccc caaagttaaa catgcaacta atcctaatat
     6721 tcccaatatt tttttcataa caatctccct ttttgtaaat ttgatacaca ttttaatatg
     6781 ttttattatg ttttatcatg ttttgttata tatagtttaa ttatatatac atgataataa
     6841 attttcaagt aatttattta cactactttt aaatattttg ctacattttc ctttttttaa
     6901 tgttaaaata accttatatg gaggtggatt atggactcat tatcatcttt gacacctgtc
     6961 gaggtagctg aattattaaa gataacaaaa aatactgtat atgaattaat caaaagaggt
     7021 gaacttcctt cttataaagt agggaaaaaa ataagagttg atactaaaga tgttgaagaa
     7081 tatatcaaca gccaaaaaac tggtaaagta aaggatacac atttcaacaa taaaaatata
     7141 gatataaata ataatttcaa aattattatt tctggtcaag atgtaatatt agacatttta
     7201 gctaggtctg ttgaaaaaaa attggatgga ataagtacat ttcgctctta tataggtagt
     7261 tataatgctt tgtatgagtt atataatggc agagtttcta tagcatcatc ccatctatgg
     7321 gatttcgaaa cggatgaata taatagcact tttgttaaaa agctattgcc aggaatacca
     7381 tgtgtactta taaatctagc ttatagaatg caaggtttct atgttgctaa aggaaatcct
     7441 aaagagataa caacctggga ggatttaaca aaaccaagta taaccattgt aaatagggaa
     7501 aaaggttctg gaactagggt tttactagat ggtaaactta gacttctaaa ctttaatggt
     7561 aaacatataa aaggttatga taatgaagaa ttatcacatc ttggagttgc aagtactgtt
     7621 tctcgtggaa tagctgatgt tggtttggga aatgaaaaag ctgctttaca agtaaataat
     7681 atcgacttta ttcctcttca caaagagcgc tatgatttag ttattaaaaa agaagactta
     7741 caaaatccag tttaccaaac tataattaac ataataaact ctcctgagtt taaagctgaa
     7801 cttcaaggtc ttggcggata tgatttaact gatactggaa aaactatagc aaaagtttaa
     7861 gattaaaatc gcaatctatt ttataagatt tataattttt cacaaaattt ataacttaaa
     7921 cacgaaataa attgcgattt tagtgtctca aaaaatttat gtctttcatt ttatcatcca
     7981 atacaaaata taatataatt aattgtgcaa tcatattata ttaatatata tttacataat
     8041 agtatttttc tttaatgtag attaacatat gatatgtttt aaacatcaac acccttgctt
     8101 tcttatatat atgtggcaac acatatgagt ttaataaatt atatacaatg ttgctttctt
     8161 cttgtatttt ttcaaccaac aagttattta taaaattacc tttctcattt tcaataatct
     8221 cactccaatt tatcttaact tttaatatat atcttcccac taggtataca cactctacaa
     8281 taaataataa aaatacaata cagtcagtac ctgttgtctt atataaatta cccgatacta
     8341 atggctttgc gaagaaatta taaaaattca aagtatcacc cgtataatca aaaagtatta
     8401 atagcactcc tagaactctc aatactttaa tattcaacat gtttttatct ttattatttt
     8461 tgtttttcat atatactacc tctcttttct ttagatttga ttgtaatcta atactaactc
     8521 ttttatttga ggtataaatg aatttaattg gtagttattt tcatttttac tagaaattaa
     8581 ttcacattac taaacaaaac ttatatttaa acataaaaat agcctatcta ttttttaata
     8641 aatacaaaaa taaataagct actaatattc aattaacttt tatcacttaa aacattacta
     8701 tctataaatt taaatatttt attgaagtat actgtactat catgttcaaa tccatcaaca
     8761 tgaccaatac cttctataag ccataggtct tttttatcat gtttaactaa atcatacatt
     8821 ttcttgctat tttgataatt acaaacagta tcttttgttc catgtataaa cagtatgggt
     8881 acattagatt tctcaactga ctcatatggt tttacatcac tgtaccaaaa tccagattta
     8941 aacttattat ataaatctcc acaaatcacc acataattga ttaatatatc tggtattcgt
     9001 ttttctagta ctcccattct aattgcatct ttcatttcag aataaggaga atccagtata
     9061 taaaaatcga ccttatcatc ttttgaattt atctctgaat gcatagcagc tgtccctgct
     9121 cccatggaaa atccatgcac tcctaatctt ccttcaggaa atttattttt tacaaattta
     9181 actagactat ctaaatcata cctttcataa agaccaaatg tgtaattatc tccaccacta
     9241 ttaccagtat ttctttgatt ataaactaat acattataac ctttatctaa atatctatat
     9301 gctaccttta acatttcata gtaactactt ccaattccat gaacaagtat catagtatct
     9361 cttgtcttta tatttgaagt tataaaaaga ctttcaagct tgtaattatt agaactttgt
     9421 atcattaatt cagatgtctt gtagtttttt aatttctcca gtggtttatc ttttctctca
     9481 gaaaatattt ttactatatc ttcgtttttt attaactgct ctaaaccaac cgaactatta
     9541 tacactaatt tgccaaatat ataagataca attaatagga ttaatagaaa cacaattgat
     9601 ataattataa atttatttct taaactaata caatttttaa aactattttt catatttatt
     9661 taccatacaa ttttcctata ttatattttc taagtaatag cataatt
//
LOCUS       DAMKPG010000119         9525 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMKPG010000119 DAMKPG010000000
VERSION     DAMKPG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.124"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            496..1701
                     /locus_tag="PC621_003204"
     CDS             496..1701
                     /locus_tag="PC621_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF4110339.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1772..1993)
                     /locus_tag="PC621_003205"
     CDS             complement(1772..1993)
                     /locus_tag="PC621_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110340.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2134..2733)
                     /gene="recR"
                     /locus_tag="PC621_003206"
     CDS             complement(2134..2733)
                     /gene="recR"
                     /locus_tag="PC621_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF4110341.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2746..3096)
                     /locus_tag="PC621_003207"
     CDS             complement(2746..3096)
                     /locus_tag="PC621_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF4110342.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="PC621_003208"
     CDS             complement(3155..4792)
                     /gene="dnaX"
                     /locus_tag="PC621_003208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF4110343.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            complement(4869..5133)
                     /gene="ffs"
                     /locus_tag="PC621_003209"
     ncRNA           complement(4869..5133)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC621_003209"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5171..5262)
                     /locus_tag="PC621_003210"
     tRNA            complement(5171..5262)
                     /locus_tag="PC621_003210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5226..5228),aa:Ser,seq:gga)
     gene            complement(5263..5718)
                     /locus_tag="PC621_003211"
     CDS             complement(5263..5718)
                     /locus_tag="PC621_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF4110344.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6038..7309)
                     /gene="serS"
                     /locus_tag="PC621_003212"
     CDS             complement(6038..7309)
                     /gene="serS"
                     /locus_tag="PC621_003212"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF4110345.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7434..7640)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7767..8648)
                     /locus_tag="PC621_003213"
     CDS             complement(7767..8648)
                     /locus_tag="PC621_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF4110346.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     gene            complement(8660..9169)
                     /locus_tag="PC621_003214"
     CDS             complement(8660..9169)
                     /locus_tag="PC621_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF4110347.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="PC621_003215"
     rRNA            complement(9296..9412)
                     /gene="rrf"
                     /locus_tag="PC621_003215"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3036 a         1667 c         1026 g         3796 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taaatttttt
      181 atttatcatg tattctttat atttaatttt taaaacttaa tgctaagtaa acctatttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatacaat ataaatacta tgctacattt
      481 taggaggatt ttattatgag caaaaaagta ttaataatgt ctgcttccac tggtggtggg
      541 cataataggg ctgctctagc tattaaagaa gaacttactt ccaaaacact tgatggagag
      601 ccaattgaat gtgaaataat agatagtttg aaattagtta ataatacaat ggataaaata
      661 atttcaagag gatacgaaaa atctgctata tatactccca aagcctatgg aagtgtatat
      721 agattatctg aaacaaatct tttgtcaaaa aatgaattta aagataatct actaataaca
      781 ttcatggcta aaaaatttaa aaagcttatt agaagcgaaa aaccagactt gattatagga
      841 acacatcctt ttccaatgat tgctctaagc acattgaaaa aaaactttaa tttacataat
      901 aatgaatcaa atgcttacac agagcatttt tataaacatt atacaaatac cattaatgtt
      961 cctccgctta tttctgtatt aaccgattat actactcatt ctacatggat acaaaatgaa
     1021 attgattact atattgttgg tcatgaatat gtaaaagaat tattggtgtt tgatggagtt
     1081 ggaccgagta aaattagaac atttggaatc cctgttgaaa aatcttttct ctctcacaga
     1141 gataaagata ttgttctttc tgagttgaat ttatctcctg ataaattaac tgttttactt
     1201 atggggggta gctttggggc tggaaatata aaagagacac ttgatgaatt actggatact
     1261 gatagagatt ttcaaatact tgtaattact ggtaagaatg aaagcctaaa agaaaaaatc
     1321 gaaaaaaaac ttatgtctcg ttatcatgat aaaaatgtat gtgttcttgg atacactgat
     1381 aagatgaatg atattttagc ttctatagat gtattagtaa gcaaacctgg tggacttact
     1441 acaactgagg ctcttttaaa agatgtacct atgatagttc cttattatat accaggtcag
     1501 gaagaagaga atcttgattt cttatctaat tgtggtgcag cacttagaac tacaaaaaaa
     1561 tataacttgt caatattact aaaagttcta atagatgacc ctagcagatt agaaatgcta
     1621 aaaagaaatg ttaaatcaat aagaaagtca aattcagccc aaaatatagc taatttaatt
     1681 ttaaatataa tttctggttg ataaaataaa taaaatccct atagtatata tattatctat
     1741 tagggatttt ttattttcta attatttaat attaaattgg ttcataaaag tcttcttcat
     1801 aatcatactg ataattttta taattatttc taaattcagc atttttattt cgtacatttt
     1861 ttttagtttt tttcttatta gaaaaactta tatcctcaaa atcatcatat ttaggtttat
     1921 cttttgattt cattttttta actctttttt ctttcattgc attatgttct tctaagttca
     1981 taattggttt catgttgcac ccctatcaaa atttttaaat gatttataca acaaattata
     2041 caatacagaa gaaatttatt caataaatct aaatcaaaat taatttgaat ttaactcaaa
     2101 aaactacaaa aatcgaggta ttaacctcga tttttatatt tctcttctgc cttctagtgc
     2161 ttttgatatt gtgacctcat ctgcatactc taaatcgcct ccaacaggaa gaccatgagc
     2221 tatccgagtg actttgattc ccattggttt tactaaccta gcaatataca tggctgtcgc
     2281 ttcaccttca atagtaggat ttgttgccat tattatttct ttgacttcct gattgccaag
     2341 tctttggata agttccttta tttttaacat atctggtcca attccatcca ttggagaaat
     2401 tactccattt aatacatgat actgtccttt aaactctctc gttctctcca tagctgctac
     2461 atctcttgga tcttccacaa cacatataac acttgaatct ctacttttat ttgaacacat
     2521 gctacatggg tctgtatctg ttatattaca acaaattgag caatatctta tttctctttt
     2581 tgcatctata atcgctttag ataaagcttc cacatcatta gtattcatat taataatatg
     2641 aaaagccaat ctttgagcag tctttcttcc tactccagga agctttgaaa attcctctat
     2701 aagtctcgtt atgggacctg tataaacttg cataaatacc acctactaga ataacccagg
     2761 tatattcata cctccagtta ccttactcat ttgacttgct tgtatatcat caatatttct
     2821 caatgcttga tttactgcac ttaaaactaa atcttgtagc atttctatat catctggatc
     2881 aactacttct ggcttgatag ttatatctat aacttctttt ttaccattaa cttttacagt
     2941 aactgctcct ccaccaactg aagcttctac ttctttactt tctaactctt gttgagcttt
     3001 ttgcatattt tcttgcattt tttgagcctg ctttaacaag ttgttcatat ttccaccacc
     3061 aggcatcatt ccgcctccaa aacctttttt agccataaac actattcctc ctaaacttat
     3121 tttcatttta ttattatatc ataatagtat tttattattt actctcattt tcatcaatac
     3181 tatattttat ttctaatatc tcttcactca ctatgttctt taatatctcc tcaccaatgt
     3241 cctttttctc tttaacttca agatttatat ctttaacttc tgatgtcagt actattttta
     3301 cattaaaact tctatttaaa acatctctta ttgttttttc taaataattt attgtatctt
     3361 gagagctcag tctagttttt gcaaaatcaa atttatcatc aaatataaca tacaacatat
     3421 tagaatatac attaaaactt tttacttcac ttaagagtgc gtatattggc atttttttat
     3481 cttttttaat ttgtttaagt atattcttcc aagatgactc tataagtata acgtcctcat
     3541 tttttacatc ttcataaact acattttctt gttttacatc tatttctcta tcgacctcat
     3601 actctttttc tttattattc ccaatttttt cagacttaaa gttccccagt tcaatcattt
     3661 tttctaagtt ctcaactctt tttataagag cttctttact ttcatcaaac attggttgag
     3721 caattttcat tatggttatt tccactaaaa cccttggatt tgaagatgac tttatatcat
     3781 cctgagttat tgataatata tttaaaatcc ttattaaatc atttatatct atatttttag
     3841 actgaatttt aagttgttct atagtttctt ctggtaatga aattatttca tccaattctg
     3901 atgaaacctt acataccatt aaatttctaa aatgatcaat taagtcattt ataagatttc
     3961 ttatatcttt gccccataag acaaattcat ttaatatctc aagagacttt ttagtgtcct
     4021 gctctacaat acattgagac aattcaaata attgttctat atttacgctt cccattaatt
     4081 caacaacatc tttataatct atcttacttt ctccaaaaga catacattgg tctaatatac
     4141 taagtgcatc tctaagagct ccttgagaat ttctagctat taaattaaga gctttatcat
     4201 ctatatcaat tccttcatct tcacaaatct ttttcattct aagggtcata tctttaactg
     4261 taactctttt aaaatcgtat ctttggcatc ttgataaaat ggttgcaggt attttgtgag
     4321 gctctgtagt tgctaatata aaaattacat atgatggagg ttcctctaag gtctttaata
     4381 atgcattaaa agctccttga gacaacatat gaacttcatc tattatatat actttatatt
     4441 tagcattggc tggagaatat ttcacacttt ctctaagctc tcttatatca tctacactat
     4501 tatttgaagc tgcatctatt tctacaacat ccattgtatt atcttttaat acagattcac
     4561 atatctcaca ctcattacac ggttcttcat ttattttatt caaacagttt actgctcttg
     4621 aaaaaatctt tgcagtagat gttttaccag tacctcttgt tccacaaaac aaataagcat
     4681 gtcctatatt atcactatat atttgatttt ttaaagtctt aataatatga tcttgaccaa
     4741 taacatcttc aaatttttgt ggtctatatg ctctatataa tgctttatgc atctttttac
     4801 ctctctttag caaaattaat aaaaaaatcc ctcattatct aagggattag tcttttatat
     4861 attaaatgct gtacaccatc tatcgactta attccatagg cgttaccaaa acagttagct
     4921 caacttaggc acccccacgg cacacaaaag tgatcactta ccgctgcttc cttccggatc
     4981 tgacggggtt catgagcttt cattgcgcag gacctaagca tcaacaccac ttatttaggg
     5041 cggccctaca gaaaataagc ctttagatgg aattcaatcc tgctatagcg gattgcaggt
     5101 tacagggcac cgctacctcc ccatctagta cagcaaaatt acaaccatat ttaattactt
     5161 tcattgtttc tggcggagag agtgggattt gaacccacga tacagtttta cccatatact
     5221 cgctttccag gcgagcgcct tcgaccactc gaccatctct ccttaatctc tttttctcaa
     5281 tgccttaaaa aattcttgca atatgatcga gcattcttct tgcaaaacat caaaagctac
     5341 ttttacatta ttattattaa aataatctaa tttaaactca tgctgttttt ctatatatga
     5401 attttttaca tgtcttgtcc ctattataag cttttttatt ctagaattaa ctattgcgcc
     5461 actacacatt atgcaaggtt ccattgtaac atataaatca caatcagtca acctccaacc
     5521 acctaatttt tctgatgctt gttttatcgc taaaatttct gcatgtgccg tagagtcttt
     5581 taacgtttct gtaaggttat gagctctagc tattatttga ttatctttaa ctataatagc
     5641 acctattggt gtttcttttt tattatatgc tttatatgct tcttttaatg cttctttcat
     5701 ataaaatgaa ctttccataa ttcctcccaa aacttataac aaacttatct tatcataatt
     5761 cattttataa ttcaacttaa ttttattttg tttatttaat tattactata tatagcgata
     5821 ttttttttaa ttagttctaa aataattttt agatattcat acaagagtgt tttataaaaa
     5881 tatttagtgt tttatgatga aaattctgct gtgtaaatta acataaaaaa actatgaaat
     5941 taatatttat aataatttca tagttttttt atttattgtt tactcataat atattctctt
     6001 catcaaagca tactctacat atacaattaa tataaattta ttttataaca tcaacaccca
     6061 tataaggtct taattcttct ggaactacta cagaaccatc tgcctgctga taattttcta
     6121 atatagcagc caaacatctt cctatcgcta atccagaacc atttaatgta tgaacatatt
     6181 ctgcttttga ttttttatct cttttaaatc ttattcctgc tcttcttgct tggaaatctt
     6241 caaaatttga acaagaagat atttctacat atctattgta acttggcatc caaacttcta
     6301 aatcatattt aaatgcagct gtaaatccca aatctcctgt acatattttt acaactctat
     6361 atggaagacc taacatttgt aacatagttt cagcatcatg agttaatgat tctaattcat
     6421 tataagaatc ttctggttta acaaatttaa ctaattcaac tttattaaat tgatgttgtc
     6481 taactaaacc tcttgtatct cttcctgctg aacctgcttc agatctaaaa caaggagtat
     6541 atgcacagta tttaattgga agctctgcaa catctaatat ttcattagca tgtatattag
     6601 taacaggaac ttcagctgtt ggtattaaga aatactctaa tccttctatt ttaaacatat
     6661 cttcttcaaa tttaggaagt tgaccagtcc ctataaaact atttctattg gccataaatg
     6721 gaggtaagac ttctgtatag ccatgcttag ctgtatgagt atttaaaaag aaattcatta
     6781 aagatctttc taatctagct cctaatcccc tatataaagt aaaccttgac ccagttattt
     6841 tccctgctct ttcaaaatct aaaataccta gacctgtccc tatatcccaa tgcgctttat
     6901 actcaaaatc aaatttagta ggttctcccc aagttcttat ttgaacattg tcttcatctg
     6961 tttcaccttg tggtacctct ggatgaggaa catttggtat tctcattaat gtatattcca
     7021 tttttgcttc tacttctttt actttctcat caataccttt tatcttatct gataactctt
     7081 tcaatcttgt cttttcttct gttacatctt ttccttcttt tattaattga ggtatttttt
     7141 tagaatttac atttaactca tttttcataa cttcaacttc ttgaagtatt tctcttcttt
     7201 tattatctag ttccactaca gcatcaaggt caaattctct ttcacctctt ctttccatag
     7261 cttttttgat atcatctaga ttttctctta ttcttttaat atctaacata ttaattcctc
     7321 ctaataattt tgataatata aattataatt ccaattaatt tacaagtatt ctagctgtaa
     7381 acttttaatt taaacaaatt tgaaaaataa aaaaagacct ccgtttaccc tataaaaggg
     7441 acgaaaatct tttatccgcg ttgccaccct aattgattat actaaaacgt ataatcctct
     7501 cagacctata acgtaggtaa accgtctagc tttaactaga agctctagag tagattcaaa
     7561 aaccattgta atgagttctc accatccact cattctctga atacaatctg ttttttacta
     7621 ttttccttca ccgcttttta actaattgtt ttatttgata atcattatat tatatatatt
     7681 aacatctttc aatactatta ttcaaatttt tttagaattt tctttaattt tttatattaa
     7741 attcactttt ttacttaatt gaaattctat cttttatcca ttgtatttga attgctcata
     7801 ttattaaata ataacgagga aggaacaaat ggaactttta tattattttc gtcaaattta
     7861 tttttgactt tttccattac atcaaagtaa acatcccagt aatcatcagt tttaacccat
     7921 ggtctagcaa ctatttgcat agtgttgttt cctatagagt ttaaacctat aattggtttt
     7981 gggtcatcca atatcctttt ttcatttata aaaatttcat tcaatatgga cttaactaca
     8041 tcaatatcac tatcatatga aatattatat gaaaaatcta tccttctttt attttgatgt
     8101 gtgtaattaa ttatattgtt acttgtcaat tgaaagtttg gtataattat ggttttatta
     8161 tctggggttt taagaacagt actaaatatt tgtatatcag aaactgtgcc ttctacacct
     8221 gagccttgaa tataatcacc tatattaaat ggcttaaaaa ataaaataat catacctgaa
     8281 cctaaatttg ataatatttc tttaaatgct aaacctatac taaatccagc agctcctaat
     8341 acagctacaa ctgatgtagt ttgtatccct ataacaccca agcttatcaa tgatataata
     8401 gataacatag atatgtatat tccatataca atgaaattat ttacaccttt actcattcca
     8461 tatttgatta aaaaccttga aaccatcttt cttactatct tagatatata gatacctatt
     8521 acaaatacga ttattgcata tataatattt ggtatattat cactaaagtt attaagcata
     8581 ttttctaaag ttcttttagt tatattacta tttgttgaag cagccttagc taatgataaa
     8641 aaattcattt aactcctcct tatgataata ttcctctgtc ctctaattct tttgttataa
     8701 cttctatagt ctcttctttt aatgtctcaa ttgtatgaat atgactacat tcagttagaa
     8761 ctgataactg tttaaattca tttgtctcaa ccttcttcat aaaattatct atatccattt
     8821 tagagtgaat attaagctct gcagttatct gaccatatgt tggatgattt acgatgacat
     8881 ccttaacaat tccacctaaa tctacaataa tctttaattc atcataaatt tctttagaat
     8941 tcctgtgatt cttacatttt atttgctttg taaaatatgg ttgattatat attatatatc
     9001 cttgaggtgt agctattatt tcaaatcctc tagctcttat aagggctata tcttgtacta
     9061 ttacttgcct acttacattt aatatctcag ctagcattcc accttttaca gggcacttag
     9121 attcttttaa aatatctatt aatttgcttc ttctttcatc agaattcata ttacaccttc
     9181 tttctaaatc aaaaacttta cctatactat ataattctat attttatctt attataaatc
     9241 aatattgatt ttatattcta tttaatatta aactttattg caaataaaaa agattacgtg
     9301 gctacgtcct actctcccag gcagtttccc accaagtacc atcagcgcta gagagcttaa
     9361 cttctgtgtt cggaatggga acaggtgtat cctctctgct attgtaacca cataatcttt
     9421 atgcttaata tttctattaa gtttttagaa tctagtaata ctagtttctt tagagttaac
     9481 actctaaaaa ctacatatat attttattga atggacatca aataa
//
LOCUS       DAMKPG010000120         9448 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMKPG010000120 DAMKPG010000000
VERSION     DAMKPG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.125"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(100..753)
                     /locus_tag="PC621_003216"
     CDS             complement(100..753)
                     /locus_tag="PC621_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4110348.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(998..2863)
                     /locus_tag="PC621_003217"
     CDS             complement(998..2863)
                     /locus_tag="PC621_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF4110349.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2868..5288)
                     /locus_tag="PC621_003218"
     CDS             complement(2868..5288)
                     /locus_tag="PC621_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF4110350.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5302..6744)
                     /gene="glgA"
                     /locus_tag="PC621_003219"
     CDS             complement(5302..6744)
                     /gene="glgA"
                     /locus_tag="PC621_003219"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF4110351.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6773..7903)
                     /gene="glgD"
                     /locus_tag="PC621_003220"
     CDS             complement(6773..7903)
                     /gene="glgD"
                     /locus_tag="PC621_003220"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF4110352.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7905..9065)
                     /locus_tag="PC621_003221"
     CDS             complement(7905..9065)
                     /locus_tag="PC621_003221"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF4110353.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2998 a         1728 c          917 g         3805 t
ORIGIN      
        1 ttagagtttt tatttgtatt taaagttaaa tttattcttg tttttccttt aatatttaat
       61 agtaaaataa caaaattata aaaaaaataa agagaaactt tataaaagct tctctttttt
      121 catctctgct ataggtatcg ttttccaatg ttctctcaaa actctttcaa cattttctaa
      181 atcattctcc ctcataaatt ttactacttc aatatgttga ttattgctgt atgtcaacat
      241 tttatataac tcatcttcat tatcactagt atatgacttt gacataaaat tatatcttaa
      301 agagttcaat agatttacta acacctgatt atctgattta gttatgtata tatcgtgaaa
      361 ttcattactt aattttaaat agctattgta atttttatac tttatagaga ccgccatcat
      421 ttctgttaat tcttccattt ttactaggtc tgattcagtt aaattttgta atgctgaaga
      481 accggctaac gcatccaata ctccaattac ggaataggtg tctaatttct ccttttcctg
      541 aaattctttt actacaaatc ccttattagg tattttttct agtaaatttt cacctgctaa
      601 cctagttaat gcttctctag ctggtgttct actaactccg atagatttac aaatctcaga
      661 ttcagtaatt ctctgattag cttcatactc tccattttgg attttctttg atatatagtc
      721 atatacttga gttgtaagtg gatctacctt cataagacgt tcctccatat taagattata
      781 aaagttaaat ctccctacaa ctttggtaga gagttcagtt ttatcttact aatttatata
      841 atatcacaat tatgatattt tttatacttc tttgtaatat tttgaaacac taaaattctt
      901 atgataattt atacaaaaaa agtagaaaaa gttttgataa aacaaaactc tttctacatt
      961 caccttattt tctatttatt tttctaaaaa aagatattta atttactcta aaaattctaa
     1021 atgatttaga gtctaaatct actttcaact cattattttc aatttgtata gtactatttg
     1081 catttttaag actatacttt ataggtattt cctcaataaa actactctct atacataatg
     1141 atatactttc cacattttca cttctattta ctaaaattaa cattctatca ttattttcat
     1201 caactctaat aaaagaaaat acagaatcat tattagtata tataaattct gtatttccag
     1261 aacttaaaag tttattctta tttctaattt ttataacatc tctataaaaa ttaagcatat
     1321 cttcgtcttc atttttccat ggataagttc ttctattatc tgggtcaaca cctccacaaa
     1381 gacctgcttc atctccataa tatatatatg gtactccttc aaatgtcatc tgtatagcta
     1441 cagcaagctt cactaaatct acatcatcat ttaattccgt tttagctctt gtaacatcat
     1501 gtgttccaat taaatttaaa ttagacctaa atgattcttt tggataattt tctttaatct
     1561 gcatatatct attacaaagt tcatatgaat taatttctcc ttttaagaaa gaaaacatgt
     1621 tatttctaaa aggataaccc ataacagaat ctaattcttc acctaataaa taacttcttc
     1681 tctgaccata gctaatctta tttgatgcat cttcccatac ttcacctata agaattgagt
     1741 catcatctgc cttttttaat tcctttttta actctcttat aaacttagtt ggtaattcgt
     1801 ctgcaacatc tagtctccag cccttaatac ccatatttac ccatttattt ataacgctat
     1861 cattgtcata tattatataa tccatatagc tatgttctaa ttcatttata tttggcaatg
     1921 tcttaacatc ccaccaactt ttatactttt gaggaaattc ttcaaacata taccatgaat
     1981 aatatggaga ttctttagat tgatatgccc ctaaactatt atagtttcca tacatattaa
     2041 aatatttact atctgctcca gtatgactaa aaactccatc taatactatt gatatacctt
     2101 tttcttttgc tttatcaata agttctttaa atgtatcttc atccccaaac attgggtcaa
     2161 tttttttata gtcacctgtg tcatatttat gattactaga agcttcaaat atagggctta
     2221 gatatagtat acttacacct aattttttta aatatccaag tttattgatt atccctctta
     2281 aatttccacc atggaaatcc catcttataa catccccttg actatccttt atgtacatag
     2341 gtatatcttc ccaatttcca tatatgaaac tatttttttt aggattatca accttaccac
     2401 tacgatttcc attattgaat ctatctacaa atatatgata aagtattcct tctttgtacc
     2461 atgttggtac tttaaaatct ttgtaaacag tgatttgata tttattaata tcactataat
     2521 tatattcaca agaatatcca ttatctctgt tttttccata aaataaagtc tttatctgac
     2581 catccatttc aacatcaact ttaaagtaat aaaaataaac acctgtacca tcaaactttt
     2641 caatcttacc acaaaagcac tttccaagtt tatcattatc taattcttca tttaaagtta
     2701 ttgttctcat ttcttcttct ctatttattt ctagggaaat agacttgata ttgtatccct
     2761 catttacttt aacacaaatg gtcaattcct catcaaattt taaagctcca aacggagcct
     2821 taaaattttt atcccaagag ttatattcta ttacactttg catttaatca caccctttta
     2881 aaccaaatgt cttttgcata ctcagaaatt gttctatcag aagaaaaaaa tccagagtta
     2941 gctatattaa ctaaagacat tttattccat ttttctttat ctctatataa cctgtttata
     3001 tctgcttgag cttgaccata attttcaaaa tctctcaata caaaatactc atcattgtaa
     3061 gtagtgagtg aattataaat acttcttcct tcttcgccaa gattaggaat aaatccattt
     3121 attaaatcat ctaccaccct ttttatatcc atattagaat gatataagtc aagtgaagaa
     3181 tatccaccat atttattata atttagtact tgttctgcac taagtccaaa taaaaacata
     3241 ttttctttac caacctgttc acatatctct acatttgcgc cgtcaagtgt agccaaagtt
     3301 atagcaccat tcatcataaa cttcatatta cttgttccag atgcttcctt agttgtagtt
     3361 gaaatttgct cactaacatt tgctgctggt attattattt ctgctaatga tactccataa
     3421 ttctctaaaa atactacttt taacttgtcc tttactctaa catcattatt tatagtactt
     3481 gcaacagaat ttataaattt aattatacat ttagccaaat aatatcctgg tgctgctttt
     3541 gcaccaaata taaaagttct tgggtcaaga ttcaaattag gattatcaag taattcatga
     3601 tacatatgaa gtacattaaa tatattcagt aactgtcttt tatacgcatg caatctctta
     3661 acttgaacat caaatatcga acttgggtct acatttaaat cgtatttatc ttttataaac
     3721 tttgctagat ttgctttatt attgtatttt atatcatcaa gtctttgtaa aacagaggaa
     3781 tcatttttaa atttttcaat attttttaat tgtaaggtat ccttcttcca tgaatcacca
     3841 attaaatctg taattaaatt gctaagttga ggatttgaac ttataagcca tcgcctatgt
     3901 gcaattccat tagtcttatt attaaattta tttggttcat cttgatagaa atcttttaat
     3961 acctctgttt caagaatatg agtatgaagc ttagcaactc catttacact atgacttgtg
     4021 acaatacata agtttgccat attgatattg tcacagtcta ttatactcat tctttttatt
     4081 ctatcctgct catatccttt attatttaat tcctctacat atcttctatt tatttcttct
     4141 attatcatat aaattcttgg taatagttct ttcatcatac ttacaggcca tttttccatt
     4201 gcttctgaca ttatagtatg attagtatag gacactgttt ttttagttat ttcccatgct
     4261 tcatcccaag ataaattttc ttcatcaagc aaaattctca taagttctgg tatgcataag
     4321 gtaggatgtg tatcatttat atgaattgct accttatcga ataaattatc aatatttaat
     4381 ttgttctttt tatattttct tattatatct tgtatccctg cacttacaaa aaagtattct
     4441 tgtttcaacc tcaacaactt acctgcatag tttgaatcat caggatataa tacctgtgat
     4501 atttcctcag tataatactt atgttttaaa gcctcttcat agctaccaga ataatttaga
     4561 gcatttgagg taagttcacc aaaatctctc tttggtattt cactcttaaa caatcttaaa
     4621 gtatttatac attggttttg gtatccgatt attggtatat cataaggcat tgccattaca
     4681 ggaaggtagt ttttatgaat tacttttaaa tgactacctt ccttgattaa ttctacctca
     4741 ccaccaaact tcaccattgt tgcttcattt ggtcttacag tttcccatgc atatcgtcct
     4801 tctgtaagcc agttatcagg cacttctacc tgataaccat ttacaaattt ttgttcaaac
     4861 agaccatatt tataccttat tccatatcct tgaccactta tgttcaaaga tgccatagaa
     4921 tctaaaaagc aagctgctaa tctaccaagt ccaccatttc ctaaggctgg gtctacttca
     4981 gcttcaatta aatcatccaa gttgattcct aattcagata aaccttctct aacttcttct
     5041 tcaatattta aatttaataa attagatttt aactgtctcc ctattaaaaa ttctaaacta
     5101 aaataataaa cttctttttt cttatctaac tttgtagcaa cccatttttt tgctatctca
     5161 tctttcaata gactacataa tacatttaac aattgttcat cagtagcttc cttgatagat
     5221 tgtgcatata agctatacat ttttacttca aatttttgct tgaatttctt tttgcttatt
     5281 gttaccaaaa aaaccactcc tttaatcaca tatgtctcta tatgtttcta tatactcctt
     5341 agctgatttt ttccaactat tatctgtttt cattgcattt tcaactaatt taatccattt
     5401 ttctttgtcc ttaaatactt tgattgcatt tttcaaacag aaaaacatct catgagcatt
     5461 gtaattttta aaactaaatc cattgccttc tccagtaaat ttattataag gtgttactgt
     5521 atctctaagc cctccagtct ctctaactat tggtaaagaa ccatatctca tagctaacat
     5581 ttgacctata ccacaaggct caaataatga tggcattaag aacatatcac ttgcagcata
     5641 tatctgttga gccaatgatg cattaaaagt aattctagct gatactttat ctgaaaaatt
     5701 agaatcgtaa tagttgaaca ttgattgata ttgatgttct ccagttccta aaacgacaat
     5761 ctgtaaatct tctctcataa gttctggcat catgtatgct attaaatcaa atcctttttg
     5821 agaaactaat ctcgaaacta tacctatcaa cggaatagat gaatcttttt taagtcccaa
     5881 tatatcttgt aatctcaatt tattttctac ttttttgtca attgagtcta catcataatg
     5941 aacaaatata tctttatctt ttgcagggtc gtttaaatca taatctatac catttaatat
     6001 accttttaat tttcctgact cttttctaag taatccatct aattgttcac cataaaaaga
     6061 tgtttgtatt tcatttgcat aagtaggact aacagttata attttatctg caaaatttat
     6121 accagctttc ataaaattta ttgagccata atactctata tctctattat caaagtattt
     6181 ttgtggtaag tccaatatat catataaaac atgcttatca aatacacctt gatattggag
     6241 attatgtata gtatacatag tttttatatt tttatacccc tcaagggttg catatctttc
     6301 ttttagaatt aatggtaaca tccctgtatg ccaatcattt atatgtatta catctggata
     6361 aaaatcaatt ctacttatag cttctaaaac tgcatctgta aagaaagtat atctttctgc
     6421 atcatctcca tatccataga tactctttct ttcatcttct cttctaaaat aaaattcatt
     6481 atctataaag taaaatttta caccatccaa ttccatttct aaaagaccac agtattgatt
     6541 tctccatcca acacgtacac taaacacagc tatttctttt agttgatctt ttaaataact
     6601 tggtatagtt gaatatttgg gcattattac ccttacatct acaccttctt tttttaattc
     6661 tttaggcaat gcataagaaa catctcctaa tcctccagtt tttgcaaagg gccaacactc
     6721 tgctgttaca taaaaaacct tcattagtca ttcccccttc taaaaaattt atttatatat
     6781 ttgtattctt ttccactaca agaggatatt tttcatctcc tcttaattct ttcccatttg
     6841 atacactcgt atttttatct aatattgcat tatatagttt tgcatttgac tttattacac
     6901 aattttgcat tactattgag ttctctataa ctgttccttt ttcaacattt acctttctaa
     6961 atatgatact atcttttaca gtaccttcta ttaaacaacc agatgctata aagctatttt
     7021 ctactttagc actatctgta taaattgtag gtgattcatt cttttctttt gtaaatatct
     7081 ttctttcaga gtataataat tcatctgcaa cttctaaatt taataaatct ttgctcattt
     7141 caaaatatga ttcaacagaa tttacacatt ttagatatcc tgtatattca taagctccta
     7201 ttttcatatt ttctgcttct tcaataataa attcttctaa ataacaataa ctgcctttat
     7261 ttacaatgtt atatatacat tcaacaaaat cttcacgctt cataatatat gtctccatac
     7321 atatgtttgc tctagggaat ttgcctaaat tatttcctac atttattacc ctattgttac
     7381 tatctaaatt tagtgttaag ttaccagcaa aatctttatt tgcattattt acatttttat
     7441 atattatagt tatatcatta tgagattttt tatggtattc caatgcttga gaataattta
     7501 aattgcatag catatagctt ggagctatta aaatatattc ttcttcactc ttttctatat
     7561 aatctagatt agctaagata gtatatatat caccttctct atgtgagtaa gtatttctat
     7621 acttagtgtt ttctggacta aaaattgata ggccaccttt ttttctactt aaatcccaat
     7681 ctttaccact acctatatgg tctgtcaatg atctgtattt ttcttttgcg aatattccaa
     7741 ctttagtgat tcctgaattt atcatattag ataaagcaaa gtctattatt ctatatcttc
     7801 cagctattgg tgtagatgct attggtcttc catagttaag tttgtttatt gctgggtctc
     7861 cttttttgtt taaatttatt atccctaaac attcatttct catcttaaag acctccttct
     7921 attaatacgc atctaggttc gatattttca tactcagaaa ctacatttat ttcattattt
     7981 acattcttta tctgaacatt atcatttata gttgcacact cacctatcat agctttttct
     8041 atagttacat tttcacctat tacaacattt ggcattatta ctgaatcaat tactttactt
     8101 cctttaccaa caacaactcc atgtgataat acagagttgc ctacttcacc taaaattcta
     8161 caaccatcag ctatcattga tctatgaaca tttgcatttt tacctatata ttgtggaggc
     8221 attgccatag tatttgtata tatcttccat ttagggtctg ctaaatcaag tgtttctgga
     8281 gattttatta tatccatatt agcatcccat agactttgta tagttcctac atctctccag
     8341 tatcctttaa atggataagc atacattcca acattatctt ctaacatttt aggtattaaa
     8401 ttttttccaa aatcatcata atttatttct ggattttttt ctgcttcctt aaaataattt
     8461 ctcaacattt tccaatcaaa tatgtatact cccatagatg ccaaattgct cttaggttca
     8521 cttggttttt cttcaaattc atagatactt gaatcttcat ttgtattcat tatcccaaat
     8581 cttgaagctt catcccaagg aacttcaatc actgctattg tagctttaga gcgtttttcc
     8641 ttatgaaatt ttaacatttt gctataatcc atcttgtata tatggtcacc agataatatc
     8701 aatacatatt ctggattata ggtatctaca aagtccatat tttgatatat cgcatgtgct
     8761 gtaccattat accaatttcc ttctttttca ttcataaatg gttgtaaaac atagacacca
     8821 ccatttattc tgtccaaatc ccaatgactt cccatgccta tatgtgaatt taatatcaac
     8881 ggtctatatt gagttaaaac acctacagta tcaataccag agtttgaaca attacttaac
     8941 acaaaatcta taattctata cttacctcca aatgatacag ctggtttggc aattcttttt
     9001 gtaaaaacac ctaatcttga accttgccct cctgccaaaa tcatagctaa catctctttt
     9061 ttcatttaat gacactcccc tcatctagta attaatattt ataattgcat ttttactttt
     9121 ataaacttta tcagtcaata aatgccgtgt ataaatgttg cttaaataat accttaataa
     9181 aaataaaata atatcaacta tacaattgat ataatgtata ccccataatg aacttaccga
     9241 cgtatacatt attttccata agcactatag tatttattaa tattcaatta tcggttaaaa
     9301 aataatacaa attttatttt ataaatataa aaacattttt tataaatccc ttgtgtcata
     9361 taaacatgct tattttttaa tttgttaaat tcgtaatagc aaaatataat ctaatatttg
     9421 ttaaataatt atccaaagaa aatcaagt
//
LOCUS       DAMKPG010000121         9387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMKPG010000121 DAMKPG010000000
VERSION     DAMKPG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.126"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(384..1787)
                     /locus_tag="PC621_003222"
     CDS             complement(384..1787)
                     /locus_tag="PC621_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4110354.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            2664..3173
                     /locus_tag="PC621_003223"
     CDS             2664..3173
                     /locus_tag="PC621_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF4110355.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            3201..3395
                     /gene="rpmI"
                     /locus_tag="PC621_003224"
     CDS             3201..3395
                     /gene="rpmI"
                     /locus_tag="PC621_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF4110356.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            3451..3807
                     /gene="rplT"
                     /locus_tag="PC621_003225"
     CDS             3451..3807
                     /gene="rplT"
                     /locus_tag="PC621_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF4110357.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            4117..4662
                     /locus_tag="PC621_003226"
     CDS             4117..4662
                     /locus_tag="PC621_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110358.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            4649..4822
                     /locus_tag="PC621_003227"
     CDS             4649..4822
                     /locus_tag="PC621_003227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110359.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            4822..4998
                     /locus_tag="PC621_003228"
     CDS             4822..4998
                     /locus_tag="PC621_003228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110360.1"
                     /translation="MPKEMNFRPHMQLGYIIQLDQIYRIKISIFIADKLTVILLKNHL
                     LVIVSILKEIIVIH"
     gene            5098..7023
                     /locus_tag="PC621_003229"
     CDS             5098..7023
                     /locus_tag="PC621_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4110361.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            7424..9328
                     /locus_tag="PC621_003230"
     CDS             7424..9328
                     /locus_tag="PC621_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF4110362.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3752 a         1041 c         1641 g         2953 t
ORIGIN      
        1 aagtgataga tacctaatta agttgtagat atctgataag aaatttttaa gttttcaatc
       61 gttctaaatt gtgagtataa aaatctacta aatttagtta tgtaaaatat aaattataag
      121 tgtagaaact aggctactag cttttatacc acttcaaaca ttactggaag tagttaagtt
      181 tacagcagtt tagttttatg tagatataat attctgtaaa tagacaaaat taaaatatgt
      241 aattaaaata tgtaagtgtc tcaaaatgaa atttttaatt tatgaggcac ttttttgtat
      301 aaaatcaaat atatttttat aactttaaca ataaaaagag gcttatctct attttgagac
      361 agcctcaaat ttttaaaact atattagttt acagcttctt ttcctatcaa atcttttaac
      421 tcttcatgag tcatagtttc tttttcaaat aagaatttag aaacagaatg taaatcttca
      481 agattatcct ttaacatctc taaagtctct ttataacaaa tttcaactat acgatttgct
      541 tccttttgaa ccttatcaca taagaaagtc ttatcatttc catcaatagt cataaatcct
      601 aagttactca taccatattc acaaaccatt tggttagcta tttcagtgac tttctttaag
      661 tcgtcctttg caccagtaga tttatgatta aatattaatt cttctgaagc ctttccagca
      721 agtaatattt ttatttttcc aatcaactca tccttagtat aaatatatct atcttcatca
      781 ggtaattgaa gaacataacc taatgcttgt cctctaggaa ctatagatat tttttgtata
      841 ggacaaattc ctactatatc acttacaaga gcatgtccag cttcatgata tgctactatt
      901 tttttctctt tttcaaccaa tgcagagttt ttagattcta gccctgcaat aactctttca
      961 agtgccttgt caaaatgttc tgatgtaata cactcactgt catctctaac tgcaaatata
     1021 gctgcttcat ttgctatatt tgaaagatga gccccattga aaccatgagt ttttttagct
     1081 aaaagttcta gatttacaga tttgtctata ggtttgtcat ctgtatgtac ctttagaatt
     1141 tcaaatctag tgtgatagtt tggtgcacct atatgaatgt gtctgtcaaa tcttcctggt
     1201 ctaagtaaag cttcatctaa taagtcaagt ctattggtag cacctattat taatacattg
     1261 gaatctttat taaatccatc catttcaact aaaagttggt ttagtgtttg gtctttttcg
     1321 ttgttacttt caagatgtct ctttgctcct acagcatcta tttcgtctat aaatattata
     1381 cttggagctt cttttctagc tttttcaaat agagttctaa ctctttttgc tcccacacct
     1441 acatattttt caacgaactc tgaaccagta acattgaaaa atgatgagtt tgtttcacca
     1501 gctacagcag atgcaagtag ggttttacct gttcctggag gaccataaaa taatactcct
     1561 ttaggtattt ttgcacccat tttttgatat ttttgtggtg acttcataaa atcgactatt
     1621 tcaaatagtt cttcttttac ttcgtctaat ccagcaacgt ctctaaatgt agtctttggt
     1681 tttgtagaaa gtgaatcatc tgtttgatta tctctttcca tttttgtctg tgcaaagaca
     1741 ggagctggat tgttgatttt cttaaaaaag ttgtttagca ttttcatatt cacacctcag
     1801 gtaattaatt attgttaatc aattttatat tttccatatt tactattttt tattatttat
     1861 ttttcatgga aaaaatatac atgttatcta aataaaaata aatttgtaag atgtatgaaa
     1921 aaatgaattt atgtatatag agatttctac ttatgtattg aataaagtat tacaaattat
     1981 aaattatgta ttgattatat attgagtaaa gtattataaa gcatgtattg attaagtatt
     2041 tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata aaaaatgttg
     2101 tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt aataggtagt
     2161 atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga atataatata
     2221 ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt tatgtatata
     2281 gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt catgtagaat
     2341 ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat
     2401 agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa aatagttgca
     2461 aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa gaagtccgct
     2521 tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat tttaatttaa
     2581 gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt tttattttat
     2641 aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa caaataaaag
     2701 ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg cctactaaag
     2761 aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg ccgaatgcga
     2821 atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa gcgagaaaag
     2881 agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga cttagaccag
     2941 gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc cttaaaaaag
     3001 gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat aaagacatag
     3061 gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa ccaacaaaag
     3121 cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa tagatgatta
     3181 actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca gcaaagagat
     3241 taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa catatattaa
     3301 ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta agtgatggag
     3361 atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa taataactat
     3421 agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc tatgaacgct
     3481 cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc tagaagcaaa
     3541 ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta tataggaaga
     3601 aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc agctgctaga
     3661 atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg tattgaagtt
     3721 aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc aaaattagca
     3781 gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt ctttgagtta
     3841 tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt catgtacaac
     3901 caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga tttattataa
     3961 gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga tattgatgta
     4021 tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa catgataata
     4081 ttatcttatt aataatataa ataaggggga gataatatga atgagaaact aatggattgt
     4141 tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa tcgaactaca
     4201 gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta tcgttacctt
     4261 aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat aaggggaact
     4321 gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa aattatagat
     4381 gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg atttttaaga
     4441 gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg ttcaggattt
     4501 tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa aaaaattgct
     4561 agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat gcattctata
     4621 ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt tacatgaggt
     4681 agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga gcaaagtatc
     4741 tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg gaacaatgaa
     4801 gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca tatgcaatta
     4861 ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt tatagcagat
     4921 aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat attgaaagaa
     4981 attatagtaa tccattgaat aaatgaatat tgcccataat catataaact gctatagttt
     5041 aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg ggattttatg
     5101 aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt tgtggggtgt
     5161 caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat attaggtaca
     5221 ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga ggaaagggaa
     5281 aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca aagtagagaa
     5341 atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg tagagcagag
     5401 tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga tatggcaaag
     5461 ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga taagcaaagt
     5521 cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc agcaaatata
     5581 tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat taagacagat
     5641 gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg ggaaaccctt
     5701 ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca agctgattac
     5761 aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat aaaagttatg
     5821 gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga acctaaaaca
     5881 gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg aatagatgca
     5941 attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa taaatctgga
     6001 gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga gattaatttc
     6061 aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa attaaaaata
     6121 attgataaag aagtaagtat agttgctact gcagatttac attcacattt ctctgataaa
     6181 ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt agtagatgca
     6241 ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga gtggaaaagt
     6301 ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat ggatatgaat
     6361 cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga aaaagatgaa
     6421 ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca ggtggcaatt
     6481 tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa accatatatt
     6541 ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg acttactata
     6601 aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca atataaggat
     6661 aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt tatgaaaaag
     6721 aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa aaatccaaaa
     6781 cacccaggaa atagaataaa agagctagca actacagtag atggtattga tgcaattgtt
     6841 gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc tggagaaaaa
     6901 gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac ctttaaagta
     6961 aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat tgaggttgaa
     7021 taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat atataagata
     7081 aagtttctaa tattagtata atgcataatg tgaaatattt taataataag agaataaaca
     7141 agacaaataa aaatatttta acatgtaaga tatagtatga actattatac taatgcttac
     7201 tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta ttagagtaag
     7261 tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa aaaggaattt
     7321 acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga taaagtgtga
     7381 tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa taaaatttat
     7441 tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa gtactaaaga
     7501 cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag ataatttaat
     7561 ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag atgcaggaga
     7621 tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa aattagtaag
     7681 atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag gagaagttcc
     7741 attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga atcatgaatt
     7801 tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa ataacaatat
     7861 accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa agccatatgt
     7921 aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg gtctgactat
     7981 aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa aggctagaga
     8041 acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag tagaagatgc
     8101 aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag ttgccattgt
     8161 acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa aagaactagc
     8221 aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa aaatagaaca
     8281 gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg gagctcatgg
     8341 taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt ggtttataaa
     8401 agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta caacttctgg
     8461 tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa gagatagaga
     8521 tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa aagaaatgac
     8581 taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa aaataaatga
     8641 atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg aatttacaga
     8701 ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag aaaactttga
     8761 agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag aaaattatgt
     8821 aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg ttggagtatt
     8881 aggtcttact actactgata aggatgaaga agacttaagt agctcaagct taagcaaaga
     8941 ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata atctagtaaa
     9001 agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga ttgttgcagt
     9061 tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca aaaaacttgc
     9121 aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata aggtagaaga
     9181 acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg gagaagattg
     9241 ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata tcataaacaa
     9301 atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt attaacactg
     9361 atttttataa tataataaat actgatt
//
LOCUS       DAMKPG010000122         9309 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMKPG010000122 DAMKPG010000000
VERSION     DAMKPG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.127"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            30..1034
                     /locus_tag="PC621_003231"
     CDS             30..1034
                     /locus_tag="PC621_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110363.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            1159..2271
                     /locus_tag="PC621_003232"
     CDS             1159..2271
                     /locus_tag="PC621_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110364.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            2694..3455
                     /locus_tag="PC621_003233"
     CDS             2694..3455
                     /locus_tag="PC621_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110365.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            3458..4276
                     /locus_tag="PC621_003234"
     CDS             3458..4276
                     /locus_tag="PC621_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF4110366.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            4456..5058
                     /locus_tag="PC621_003235"
     CDS             4456..5058
                     /locus_tag="PC621_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF4110367.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    5619..5857
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5921..7840
                     /gene="thrS"
                     /locus_tag="PC621_003236"
     CDS             5921..7840
                     /gene="thrS"
                     /locus_tag="PC621_003236"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF4110368.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            8608..9036
                     /locus_tag="PC621_003237"
                     /pseudo
     CDS             8608..9036
                     /locus_tag="PC621_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3769 a          922 c         1679 g         2939 t
ORIGIN      
        1 tatcgtaaat gtatgaggag tgaaatttaa tgcgtttcaa aagaatattg atattgatgt
       61 taactgtagc aatgatagct ggaatgttgg ttgggtgtgc taataaccca agcaataatc
      121 ctagctctag caaagataat aaaagtgata taagcgataa aagtaattca aaaaaatctg
      181 tatcaatgat actagatatt gaaggaacaa ataatgaagc tatgaataat gcagctttat
      241 tagcattaaa caatgctcaa aagaaactta atatagatac aaacaaagtg gaatcagatg
      301 attcctcaac tttttctaat agtatagata ttttatgtaa tgacaattac gatttaatta
      361 tagctgtagg agctagattt gcaaaaccat tggagatggt agccaagaaa tatccaaagc
      421 aacaatttgc aattatagac tatgagtatg acaagcaacc ttctaatata acttcaattt
      481 cttatgaaga taataaatct ggatacctag ctggattaat tgctggaaaa atgactgaaa
      541 gcgataaggt tggttttata ggaggaataa agagttctag tagggataaa tttgaatcag
      601 gttttagaga gggtgttaaa ttttctaata gtagtataaa ggatatttca gttgagtatg
      661 cagatgtttt taaagatagc aaatcagtag aatctatagc taaaaaaatg atggataatg
      721 gagtggatat tattttttct actactgaag atgattcaaa agctgtaata gatgcagtaa
      781 gggcaaaaaa taaaaaagtt atagcaacga ataaagacca acatgagtta gctccagaaa
      841 atgttataag ttctattgta aaagattttg agaatcctac ttataatctg atacaaagtt
      901 ttgtaaaagg aaattataaa ggcggaaaag ttatagaaaa cacagttaag tctggaagta
      961 ctgggcttgc ccaaaatagt tctcaaaata ttcctccaga tgtgttagaa tatgttaata
     1021 agaacaataa ataatctact ataatatcca agaatactta tgactttagt tatgaggtga
     1081 attggatgtt ataatagaca tatatagaaa gacgtatgca gaggtaggca gacctatcta
     1141 aatcagggag tgaattttgt gaaaataaaa aggatattga tggcttgctt tgtagtagtt
     1201 tttacaattt ttatattagc tatatgtaac tttgattttg gatttgataa agtaaataag
     1261 aataattcta atatagatag tgctaaatcg aaacagagtg agtctaaaag tgtaccaact
     1321 tcagcagaaa aggataaatt aaatagtaaa agtaagaatg aagaagatga cacagaagat
     1381 gattcatatg taagaaaaac tattggagta ttgttagata ccgaaaactt aaataacgaa
     1441 tcatataata ataatgtact atcagcctta aaaagggctg atgaagattt tgatgttagg
     1501 ataaaacttg aaaaaccaaa gacttctaaa agttttaaag agagtataga aaaactacat
     1561 aagtatgaac cagatttgat aatagcagtt ggagctagat ttgcagagcc aattaaagaa
     1621 gctgctacta aatatcccaa acagcaattt gcaatagttg actatactta taaagttcag
     1681 ccagctaatg taatttctgt atcatttcaa gataatgtta ctggatattt agcagggctt
     1741 attgcaggta aaatgactga tactgggaaa gtcggtttta taggcggagt tgagggttct
     1801 agtagagata agtatgaatc tggatttaga aaggggctta aaacttcaaa taaagatgct
     1861 gagttactag ttaagtattc aaatatattt gaaaaccctg aatcagctag aactattgca
     1921 aaggaaatga gagattctga tagagatgtt gtattttcag ctacagaaga tgatagtaaa
     1981 agagctattc aggtagcaag agaaaatggt ggtaaagtta tagctattaa taaagaccaa
     2041 tacgatatgg ctccagataa tgtaataagt tcaattataa aagatgtaga acaacctgtc
     2101 tatgaactga taaagaacgt tgcaaaagat ggttttaagg gaagtaaagt aatggaattt
     2161 aaaataaaag atggagaact tggcttgact tcaaagagca gtagaaatat accaccagat
     2221 gttttggaat acataaaaaa agagtataac aaagtgaaag atacatatta attattgatg
     2281 tagaagatta taaaaatata ctgtttaagt ttagattagc aataaaaaga tatatttatt
     2341 aaatttgtgc taactgtaca aaatatattg attaagttta gattggtgat aaaaagatat
     2401 atttattaaa tttagtctaa ctgtacaaaa tatattgatt gagtttagat tagcaataaa
     2461 aagatatatt tattaaattt gtgctaactg tacggaatat actgcttaag tttagattag
     2521 tgataaaaag atatatttat taaatttgaa ccaactgtac aaagctatac taattaagct
     2581 tagattaact atataaaatt ataacaataa gtgtgtgggc agaaaaattc tgctacacac
     2641 tttatttata ttcatataaa aaaattatca aaaaaaagaa aaaggagtaa aaaatggaat
     2701 ttacaagaga accatttata ctagatgacg atttgtgtct agctttggtg gacaaaagaa
     2761 ttagttatct aatggaaaat gaattaaaat taagaaatat agagataata aaaacaatag
     2821 aatgtaaaga attatatgaa agcataaaat atcacccgga tatatgtatt tgtaatttag
     2881 gaaaaggaga tataattgtt gcgccgaatg tatataatca atacaaagag ttattaagta
     2941 gatataaatt taatataatt aagggagaag ctaatttatt aaatagatac ccttatgata
     3001 taccttacaa tattgcgata gttggtgact atgctataca caattttaag tatactgaca
     3061 aagcaatatt agattatatt gaaaataata aacttaaaaa gataaatata gagcagggct
     3121 attctaaatg ctcaatctgt atagtagata gaacgtctat aataacatca gataaaggta
     3181 tttggaaaag tatgaagaat acagaaatag agtgtttatt gatagaaaaa ggtcacataa
     3241 atttatttga gatgaattat ggatttatag gtggatgtac agggttgatt tctaaggata
     3301 aactagcttt ttgtggagat gttaaaaaac atcctgacta tgaacgtata aaaagttttg
     3361 tagaaagtaa aaacaaggag atagttacac ttagctgtga aaatttacta gatataggtt
     3421 ctatagttcc tctcatgaca agaaaggagc gataactttg gttgagaagg ataatatttc
     3481 ttccatgaaa tttttgagga ataataatgt aaacgataga ttaatatata cgaatgacat
     3541 tgtagaagta gtgatagatt ctgcaaagca agaagtgtta aataaaaaaa tacttaatgg
     3601 tactaaagct gaacaaagag ctttaaatat tgatgagata tcaacggaaa taactagtat
     3661 gataataagt ataatgaaag agaaactttt gaaagaatac attctaaaat gctatgggcg
     3721 tacatatcct gaagataaaa ataatatata catatattca ctgaacatat ttgctaaaaa
     3781 agagattttt atgagagaaa cagtatttac tagggtgaga aattatatag aagaaaatga
     3841 ttttataaat gtagaaggtt ttattagatt tagaatgaga gaatttatga aatatatttc
     3901 tgctataggt gatattgcag ttgaggagta tttaataaag aaagaccaag atgaatttat
     3961 aagagtttta aaatacttta tagatactca agaagaaaag attgatttgc ttaaagttca
     4021 tataatggaa gataatacat ttgttttata cgataaaaat ggaaataaga tagacagtat
     4081 agatgatgaa gaaattataa atatggtaat aagagaaaat ttaaattatg aggacttttt
     4141 aataagtacc ttattaactc tatgtcccaa aaaaatagaa atattggatt tgttaaataa
     4201 taattgctca aaggaaatta tagatactgt agaagcaatt tttgaaaata aagttagtat
     4261 aattatggaa aactagaatc taagcatata aaagctagcc atataaaatt aaaaaaattc
     4321 tttttaaatc gacacaaaag acttataatt gtatataaag ctatagtata actagagtta
     4381 ttaagtataa agaatatcta cttgattaat gcaatatagc tggcaaatat acaattgata
     4441 aggagtaatt ttataatgga taatttaatt aggatactga tattagctgt tattggtgga
     4501 tttataggtt atgttacgaa tgtagttgct ataagactga tatttagacc aatagagcct
     4561 attaaaatac ctattttaaa tatagaaata gtaggtctta taccaaagag aagagcagaa
     4621 atagcagcaa atgtaggaga aataattcaa gaagaatttc tttctatgga cgaaatactt
     4681 gcaaatatca ttactgacga agataaggaa gaagttgtaa gatacataaa agcaagggta
     4741 aaaattataa tacatgagaa agtttcattt atcccgagtg gtataaaaaa catgatacaa
     4801 gattatctag gagagataat tgagtctgaa gttaagcaaa gtatagatga attgagtaaa
     4861 aatataataa ataaagcaaa tgaacgtata gatatacaaa aaatggtcga agataagatt
     4921 aatgaactag atttatatga attggaagag ataataataa gaatagctaa gaaagaatta
     4981 aaacatatag agtttttggg gcttgtctta gggtttttaa taggtatagc tcaagggtta
     5041 attactatgt ttatttaaat atatgagaag tagcatttat ttaagtacta attatattta
     5101 cctatttgag tgaattactt attaatagta tttagatatt aattgtattt acctatttga
     5161 gtgaattact tattaatagt atttagatat taattgtatt tacctatttg agtgaattac
     5221 ttattaatag catttagata ttaattgtat ttatttattt gattgtgtta cttattaata
     5281 gtatttaagt atttactgta ctacttatta actatattaa gtattgattg tatttaagta
     5341 tacaaaacct aaaaagagta caaaaattag aagttagttt taataaaaat tagttttagt
     5401 atttatctag tcatatctat catgcatgtg gatacgtttt aaaaatttta aatttatgaa
     5461 aatattataa tatcactata aatcaagaga ttaatttaat ataaaaatat aagaatattg
     5521 gtttacagct gattgagatg aaaaataatg ggtaattaat atattacatt aatgttgatt
     5581 gatgtataac tatctgttat aatgaaaata tattaaaaat catagtgttg aagaggaaga
     5641 gtagagtaga aaccattctc agagaatttg ggtctatggc tgagagccca tgtaataggt
     5701 ctatttgaaa actaccttgg agctgagctt attagcagta tttgtatttg tactatacgt
     5761 gaaagtgctc gggtgacgac cttatagtca tcaagagggt tgtatatact tgtatacgcc
     5821 aacttgggtg gaaccgcgaa ttaatatcgt cccaaacata tttggggcga ttttttatta
     5881 caaaaaatct aaaaattatt tcaataagga ggaaaaaaca atgattaaag ttgctttaaa
     5941 agatggctca ataaaagaat ttgaaaatgc tatttctgtt atggatgtag ctaaatccat
     6001 tagtgaagga ttagctagaa atgtggtagc agcgtctgtg aacggagagg ttgtaggtct
     6061 ggaccacatt atagatactg attgtgactt gaatttattt aagtttgaag ataaagaagg
     6121 taaggaagtt tttagacata catcagcaca tatattagct caagctataa aaagacttta
     6181 cccagaagca aagcttgcta taggaccaag tatagaaaat ggattctact atgatataga
     6241 tttagaccat agattagttc cagaggacct agagaaaata gaagctgaaa tgaaaaaaat
     6301 agctaaagaa gatttaaaaa tagaaagatt tgaacttcct agaaatgaag ctttagagct
     6361 tatgaaagaa caaggagaag actataaggt agaacttata tctgatttac ctgaaagtga
     6421 aataatatct ttctacaagc aaggtgattt tactgattta tgtagaggac cacatttacc
     6481 atctactaaa aaggtaaaag ctgttaaatt acaaagtgta gcaggtgcat attggcgtgg
     6541 tgacgaaaat aataagatgc ttcaaagaat atatggaact tcttttgaga aaaacaaaga
     6601 cttagaagag tacttacatt tactagaaga agctaaaaag agagaccaca gaaaattagg
     6661 taaagaatta ggattattta tgatacctga agaaggtcca ggattcccta tgtttttacc
     6721 aaaaggtatg gaacttaaaa atgaattatt aaaattctgg agagaaatac acagaaaagc
     6781 tggatatatt gagatagaat ctccaatcat attaaataga aaattatggg agacttcagg
     6841 acactggtat cattacaaag aaaatatgta tacagttaaa atagatgatg aggattatgc
     6901 tataaaacct atgaactgtc ctggtggatt aatttactat aactctcaac tacattcata
     6961 tagagatttc ccaatgagag ttgcagagct tggtagagtt catagacatg agttatctgg
     7021 tgcattacaa ggattaatga gagttagagc atttactcaa gacgattcac atatattcat
     7081 gttaccagag caaattaagg acgaaataaa aggagtagct aacttaatag atggtatcta
     7141 caaaactttt ggatttgaat ataatttaga attatctaca agaccagaga actctatggg
     7201 aagcgacgaa gagtgggaag cagcagaaaa tggattaaga gaagctttag aagagttagg
     7261 tctaccatat actattaatg agggtgatgg agcattctat ggtcctaaga tagacttcca
     7321 tctaaaagat tgtttaggaa gaacttggca atgtggtact atccaattag atatgcaact
     7381 tccaagacaa tttgataata cttatattgg tcaagatggt gaaaagcata gaccagttat
     7441 gattcatagg gttgcttttg gtagtataga aagatttata ggtatattaa tagagcatta
     7501 tgctggtaaa ttcccagttt ggttatctcc aactcaagtt aagatacttc ctatatctga
     7561 taaatttatg gattatgcaa atgaagttaa gaaagagtta tttgataagg gtataagagt
     7621 tgaattagat gatagagctg aaaaaattgg atttaagata agagaagctc agcttgaaaa
     7681 agtgccatac atgttaatag taggtgaaaa agaagtagct gataataatg tttctgttag
     7741 atcaagagat aaaggtgaaa taggtagtat taaattagat gaatttatag ctagtatttc
     7801 aaaagaaatt gaatcaagag aaagtattat tcaagactaa tatataatat attagtagct
     7861 aagatattgt tatataagat aagtttaatc aatatgttga tatgttgata catagtgatt
     7921 aaatttatga aaagatgagt ataattagag gtacagttaa tcatattttt atgattaatg
     7981 tacctctagt tttttgtatg gtagatttat taataaatat atcatagaag tgttttgaac
     8041 atggaaatat ttatgtgcat tctttaaata aaagtttatt ttttgaatga taattaagtg
     8101 cttgttggta aggttatggc tgtaaaaatc ataactttat ttttatattt gttccatttt
     8161 cataaaaatt attatccctt taagaaaatt tcttaatcct cttgaaatat ttccttttta
     8221 gtctaaagtt aaatttcttt cttcaaaatg aatatttaaa tgaactaaaa tttaagtaca
     8281 ttttctaatc ttatgagtct atttttcata gataattgcc ctataataga agaatctatg
     8341 ttggggctat gtggtgacaa taacagcatt gtcagtgatt aattaatgct ttataaatga
     8401 ttaattagtg ttttatgtta tttttggact taccctctat acatagatta atttatttaa
     8461 ctttagatat agagagaatg tcaagtacat acacagacat tagagtttaa gaaagtgttt
     8521 atggagcaag aacttttaaa agaagtagtg acttagttta atttcaagaa atgataaata
     8581 caaaagagga aaaagagagg tgttgaaatg atagaaaaag tagaagaaaa atctaagatt
     8641 attaaaacat caaaagaata taagcgaact ataattgaag aaaaagataa agaatggacc
     8701 aaaaaattaa atgatatact tagtagaagt agtttttgta atgatgaatt agataaaatc
     8761 aaggcgttaa ttccaaaaga gattttgact aatgaaaatg ttggagaagt tgtgactgaa
     8821 gatggatatg ctaagcccga gtatgatgag gtatttaaat cattgttcac tttaaataat
     8881 gaatatgatt tacttgtaaa ttttataaat gatattctaa aagatgctcc atatgctaac
     8941 aggaatatca aaccgttcac tcaaataaag agaataatta gagttgaaac tgatccaaca
     9001 attaattaca ttggagagaa aagacctaga cttgattaaa aaatagctac gcacacttgt
     9061 gactttagtc atgagttagt agctaaaata gtcagcatat agggaaactt atatgtagag
     9121 ataggataga acctatccaa tactacttga attgctaaaa acccctaaag ctaaccaaac
     9181 taaaacataa ggatgaaata aacctaagtg tgaaagttac gaaagtagaa aaaattggtt
     9241 agatggtata aggttaaacc ctaagtactt taaaatgggc aatcagcagg taagctccga
     9301 ataggagaa
//
LOCUS       DAMKPG010000123         9239 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMKPG010000123 DAMKPG010000000
VERSION     DAMKPG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.128"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..655)
                     /locus_tag="PC621_003238"
     CDS             complement(<1..655)
                     /locus_tag="PC621_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF4110369.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     "
     gene            complement(648..1019)
                     /locus_tag="PC621_003239"
     CDS             complement(648..1019)
                     /locus_tag="PC621_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF4110370.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(1201..2547)
                     /locus_tag="PC621_003240"
     CDS             complement(1201..2547)
                     /locus_tag="PC621_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF4110371.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(2729..3322)
                     /locus_tag="PC621_003241"
     CDS             complement(2729..3322)
                     /locus_tag="PC621_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF4110372.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(3387..4856)
                     /locus_tag="PC621_003242"
     CDS             complement(3387..4856)
                     /locus_tag="PC621_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF4110373.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(4971..6362)
                     /locus_tag="PC621_003243"
     CDS             complement(4971..6362)
                     /locus_tag="PC621_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF4110374.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(6528..6776)
                     /locus_tag="PC621_003244"
     CDS             complement(6528..6776)
                     /locus_tag="PC621_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110375.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            7042..9171
                     /locus_tag="PC621_003245"
     CDS             7042..9171
                     /locus_tag="PC621_003245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF4110376.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         3036 a         1495 c         1027 g         3681 t
ORIGIN      
        1 ctgtaattgt accagaaact ccaatagaaa caccactcat agctccttct atttctccaa
       61 gttcaaaagc ttttgtagtt cctaaagcat gtgcagatgt tccaagagca attcctttag
      121 caactggatg atttatattc cccagtttga atactgtaga agcaaccata ccaccaaaaa
      181 tacctgtaaa aattatggct acaactgtta ttgattctat accatttaaa gaatttgcta
      241 aagatattcc cattggtgtt gttatagatt ttggaattag tgaatttatt atacttacat
      301 ctgaattagc tattttgcca attatcaata cagatacaaa agaaatcact ataccacaac
      361 ctattcctac aataatctca aacatatgct ttttaaataa ttcaaattgt ttataaagtg
      421 gaactgcaag tactactgtg acaggaccta aaaagaaatt tagactatca gctcctatct
      481 tgtaattctc ataagggatt ttagatatgc ttaaaaacaa tattattcct acgattgcaa
      541 ttaataaagg gttacatata ggattacttg tttttcgttg aatgtatctt cctaaattga
      601 aaaaaactat tgttactatt ataccaaata ctggtgtctg taatacatta tacatttttt
      661 tgtccttctt ccttcttttt tatcattttc tccacaataa ttccagtagt atacattact
      721 attagtgtac ttaaacatat gactaataaa agcactaata cattttccct tattaggtct
      781 aatgacttta taagtgatac acctgctgga ataaaaaata tagccatatt atcgaggaaa
      841 aatccactta cattttctat actactcaag tctaaaactt taaattgtag tagtagaaat
      901 aataatagca taccaactat actcccagga ataagtataa ttccttgtat aaaggaactt
      961 atatactctc ctattaccca gatagataaa attattacta attgattgaa aactttcatt
     1021 ttccacctcc aaaaattaaa gagattgctt caaatcatat atttgtagtt ttatcaacaa
     1081 gcaaatatct ttctttaaaa tagaaagtta taatttgaca caatctcttt ttaaatttat
     1141 ttttatttta atttaataaa atacatactt ggttgtttca aaaaacttaa ctatataaac
     1201 ctatttttca cttaagattt cttttatttt tgaattgtta tacatactgt ctggaacttc
     1261 acttctcaaa ctataaaaag tctgttgtgc attttttaaa tcgccattat cataataaag
     1321 catacccaac tcgtaatatg aatcatcata atactggtca tttttagtat aagtatttat
     1381 atattttttg taatatttta tagcttcatc cttatttcct aataatttat ttgttattgc
     1441 taattgatat attgactcag aaatatattt tttagtttta ccagaagaaa ctacactttt
     1501 aaaattatct atggcttttt catattcttt gtttttcttt aaatttagag cttgataata
     1561 taaatcttcc tcatcagcta cagttatctc tctttctggt tctttatttt taacctcatt
     1621 taattcctta ttcgtttttt ctaatttttt actcgtttct cctaactttt tatcagtttc
     1681 acttaacttt tcctctttac tacttatttg gttattcaag ttttctattc tctttttgct
     1741 ttgaatcaaa tatatcccca tacctattaa aacacagcca actataatat atggtaaaat
     1801 acttttcttt ctttttactt ttcttatttc ttccttttta cttgtatctt ctttttcttc
     1861 ttcatttttt tcaaatacag gattatcttt atctagctct ttaagtttgt ctaaatatcc
     1921 ttctctcttt tttacattac ctagtttatc atataacaaa gataagatta catatggctc
     1981 tattagctca ggctcttctt caattatgtt ctctaaaata tgtattgact cataatttaa
     2041 ttcttcattt ataaatctta tagaatgatt gtatcttcct aaaaaagttt taaaatcttc
     2101 agatgaaatt agatttacat attttctaga tatatgatta ttctcataac ataaagcagt
     2161 ataaaaactc tcaaatgatt tagcaaaatc acattttaat aactttaaaa gacctctaag
     2221 atttagtata tcaatatctt taggatttat ctccagagct ttattaacca tattaatggc
     2281 actagttata taatttttat atgctaaatc caatgcattg ttatatcttt tatatgattc
     2341 taattttaac tccttagacg ttgtgtctga ctttgaattt aaagctcctt gcaagtttaa
     2401 tagagtttct tctttataat ttgtagtcac ataactctgt tttaatgctt ttaatgtttc
     2461 atcaccgtct tcttcttcta tattaaaaaa agtcaaatca cttttattat cttctatcaa
     2521 agatatatct aaaatttttc taggcatgct ttctatgtct tgttttatat ctttaactaa
     2581 aataatactc acctccactc taaaatatat atttaagata acatatttaa tatgtcaagt
     2641 caaaactata catatttttc caccattctc ataataaaaa aggtaatcct cttcttttca
     2701 taaaaaggtt acctcttttt caatactatt aacttttaat gttgaatttt tttacttttc
     2761 tatagagtgt agctatacct atatttaatg cctcagctac ttttttcata ccttctgtat
     2821 tacttccata tttctttatg gcattttcta tagcttccct ctcaaggtca tctatagtct
     2881 taacttcttg tttataaagt tcatctagtt taaaagatat taaatcagac atactcctta
     2941 caaaagcagt taactcctta tcttttgaaa gtatcttttc tttttgctct tcattaaacg
     3001 cagctatacc caagattcca agtatatgtt ctcccatttt aacaggagta catatatcag
     3061 ctaattcttt acaattttct ctatttgcac atttattaca gttctcttct atttccttat
     3121 ttataacata ggattttcca tcttttaaaa caagagagta cactgacttc tctgatattg
     3181 tctctcctat tttacctctg aatattcctg tacctgctat cctagttaaa tcttttgtaa
     3241 ctaaagtaac atctacatcc aatacattag aaatattctc gcaaatactt tgaacgtaat
     3301 tcgttatatt atttaaattc aaagtaatcc ctcctattta gatttatatt ttttattact
     3361 ctacataaat cctattcttg agaactttaa gcttgcggtt gtggttgtgt ctcattaggc
     3421 ttattatcag ttggtggtgt ttctggtttt tgttgattat ttggattttg attgtttgta
     3481 ttttgattat tcttattttc gtctgtattt ggtttttcct ctggttttgg ttccatagtc
     3541 ccaactaata taataccttc cttcttagga taatatgaat tagctatttt ttcagtttta
     3601 atttttttac catttgcatc attaaatgtt ctatatgttg atacagaata tgcatttctt
     3661 ccacttttgt ctactttttc tttaccttta ggcattgtag ggtcattaac tcttttcatt
     3721 gtagtttgtg aaacgccatc taattttgtt gatatcgtta tattttgttt atcttctgca
     3781 cttccatata tattgcaaac aatttgattt cctgaaacgt aattttttat gtaaactgga
     3841 tgctttagat tatttttaaa taagaaatct attccactat ctgcaacagt tgcatctctt
     3901 ccttttggaa cataacttga tggtatagta tgatttttta cattaacaat ttctaaacct
     3961 gcgtataaca cagaattgta taacgtagat gaaacttgac aaactcctcc accataatct
     4021 tcttccatct caccttccat tattacaggt gcacttttat atccattttc cttacttctt
     4081 ttccctgtat gtttattaaa tgagaaagtt tctcctggca taagtaatac attacttata
     4141 ttatctgttg ctaattttat gtttatactt ctatttattt gagaagtttt aaatattgtt
     4201 gaatacgtac ctaaatttgt atctatatta gtaagttgct ctttttttac ttttgctgga
     4261 gtagctttta ctactaactc atttttatag ttaccttttt tagtttgatt atcaaaattc
     4321 tccatactct tagaaatatc cattttaaga ccatcagaat ctggtataac cttgactttt
     4381 tcaccactta tatcaagtgt agcatctttt acatctacat ctatttcttt agctattttt
     4441 tccatttctg cttttaattt atttttatca taatttatca caactactaa gttgcttttt
     4501 ttgccaaaat ttgttgaaat agtttttgat agattgccaa agaaactact ctttctattt
     4561 aaattatatg cgttttctac tgttttatcc agatcagtag ataaatttaa atctttgctc
     4621 tttactttcc aacttttatc attgtaatta aactcaacat tctctaattt gtatttttta
     4681 gctaattcct gttttgcttg cgatttagtt aattttccaa tatctactcc attaacatat
     4741 gtgttttttg ctattttctc ccctttaaat tgcatgcaca ttatgcctat aaaaattatt
     4801 gctactaatg ctacagctcc aatagctata actagctttt tgttcacatt actcatccca
     4861 tacttctcct tacaataaaa ttatttatat atccctatta ttatattact acataatgcg
     4921 ccttaaaacc ccaaaaactt tttaattata tattaattta atattattaa ttaatattca
     4981 tttagtccat tttctgatat ggcatatatt ttttcattgt aaatattaat agcataaact
     5041 tctttgcctc taaataagcc tttatttact acagcataat aataaatatt tttatatttt
     5101 ccaaacaatt cttctaaact ctttttactt tttacatatc tatctgcttt ctctatatct
     5161 aatatctcaa tctttttata cttattttct ttttcatcaa gagtattagt taacacaaac
     5221 ttagctttat cttttgtaat accaaattga taacttccat cttctaatat tctaaagtat
     5281 ccatcaccat aatctacttt gtaatctttt ttaccatctt tatacgaata taaatcttta
     5341 gtttttgcac ttaccaaaac ttcatttatt aatttatcat actcatatac ttcagcataa
     5401 tagtatttat tattttttat catttgtgga agtgaattta tagatagatt tgccttgtct
     5461 ttgccacttt ttttagctat tacatttata atgtcatcag atgttaaggt atacaatgat
     5521 aaattctttt tatatttttt ttctaaactc tgtacctttt catcatctga ataatcttct
     5581 tttaaatact caatatattg taatgctctt tcataatctc cagaagtatt tgcctcttca
     5641 gctctactta tatagtaatc atacatttct tcaatacatt gttctttccc attttgagcc
     5701 atactgtaat acttttcatc ttcttttaat actttattat acttttcaat agcttcataa
     5761 tacttatatg ctttttggtc ttttactgct gagtcataaa catctctaga ctctttgatt
     5821 gtttctatat tctgtttgta tacatctagg ttactaccca taccatttaa tattgaaaca
     5881 gtacttatgt agttaatggc aatatcataa gtagtatttt cttctttata catttctcta
     5941 actctatcag cttgagctaa taagtcatcc acatctatac ttattttgtt taattctttc
     6001 acagtattta ttaatccaat aaaactttct tgacttatgt ctccatctaa atatttatca
     6061 ccactattta atagcaattt atttattttt ttagaaacag acttattaaa cctttccatt
     6121 tttgaatcag aaaacttttt ttctatactt gtgtagtaat caacagctat agaatactgt
     6181 cttttctcta taatctttaa caactcatta gcttcatttc ctacactaaa ctcgttacta
     6241 cttaagaaaa atactattat caaagcaatt acacctaatg ttgataatga aatcgcaact
     6301 atagatttcc ttttttcatc aataaaatct acagccttct caaataaatc tctaaaattc
     6361 attatagttc cctctccatt taataatctt tatattttat ggtaaactaa atttcttatt
     6421 ggttattata tcataattct tgaaaaaaaa taaaaacaca agttaaatcg tgtttttatt
     6481 ttacctatca atcattattt tatacataaa tatttccttt acataaatta tgatatcttt
     6541 ctttttatat ccctagaatg actcttttgt tttttattag tactgacagt tttcgttctt
     6601 gtcttgtgta tagctttgct atcaaatatt tttctagcat tgcataaaac aattccaatt
     6661 gttaaacaaa ctgtaagcac acttcccatc aaaaagtaat aatccaatcc tatatgtact
     6721 cccataactg aatttattaa cactaatgca aaagtcattg caaaacatat catcatggag
     6781 ataactaaaa ttcttagaat ttttttcaat ccaatcacct cgtataatca ctaaaattga
     6841 cactacccct tatccattac tataccaaat tagtaaaaaa atagcaatta ctattgtcat
     6901 ttatattagg aaaatattaa atctttttaa taaatatctt tttttgacat aaggattact
     6961 taacttttaa taaaatagta acactaatat gtattaatta atagaataaa ataaatgata
     7021 atttattgag gggtgattta catgccaaat ttgccaagtt tagggtcaaa ggctcctgat
     7081 tttaaagcca atacaacaaa tggtcctatt agactctctg actataaggg taattggatt
     7141 gttttatttt cacatcctgg tgattttaca ccagtttgta ctacagaatt tttatgtttt
     7201 gctaaatatt atgacgaatt taaaaaaaga aatacagaac taattggtct aagtgttgat
     7261 agtaacagtt cacatttagc ttggatgtat aatatttctt tacttacagg tgtagaaatt
     7321 ccatttccta ttatagaaga tagagatatg agaattgcca agttatacgg catgatatca
     7381 aaaccaatga gtgatacatc aactgttcgc tctgtattta ttatagataa taatcaaatt
     7441 ctaagaacga ttctttatta tccactaact acaggaagaa atattccaga aatacttaga
     7501 atagtagatg cacttcagac tagtgataga gataatatag ttactcctgc aaactggttt
     7561 cctggaatgc cagtgatttt accttatcct aaaaactata aggaattaaa aaatagagtt
     7621 aacagttgta ataagaaata ttcatgtatg gactggtact tatgttttgt accagataat
     7681 tataatgatg aagaagtgag caagaaaatt gataatactt gtagctggaa aaaagaacat
     7741 actaaaaaca ttgaaaatga atgtaactgt gaacatgaac atcatgacta cctgaacaaa
     7801 gctcttgact gtaaacaaga acacaagact gatattaaag atgattgcaa ccatgagaaa
     7861 aaacatacta aaaatactaa caaagttcac aactccaaac aagataagtt taaagataag
     7921 tcttgtgatg aaatgaattt taactatgat aaagatgaat cttgcgacaa aataaattct
     7981 agctataaca aagaagatag tagttatgaa gatttctata aacataatta taaaaactac
     8041 gattatacta gcgaaaaaaa tactaaaaaa atagctatga aaactttaaa agattcaaaa
     8101 aaattagtta gaccacaaat aacagaccca tacaatccaa tagttgaaaa tgcaaactgt
     8161 ccagatataa atccaattgt agcagaatat gttcttggaa atccaactaa tgtagatgct
     8221 caactattag atgcagttat atttgctttt gctgagatag accagtctgg aaatttgttt
     8281 attccttatc ctagattttt aaatcaatta cttgctctta aaggtgaaaa acctagctta
     8341 aaagtaattg tagctattgg aggttgggga gctgaaggat tctctgatgc agcattaaca
     8401 cctacatcta gatataattt tgcaagacag gtcaatcaga tgataaatga atatgcttta
     8461 gatggaatag atatagactg ggaatatcct ggaagtagtg catctggaat aacatcaaga
     8521 cctcaagata gagaaaactt tacactctta ctaactgcca taagagatgt tataggagat
     8581 gataaatggc ttagtgtagc tggaacagga gatagaggat atataaattc aagtgctgaa
     8641 atagataaaa tagctcctat aatagattat tttaatctta tgagttatga ttttacagca
     8701 ggtgaaacag gcccaaatgg tagaaaacat caagcaaatc tttttgattc agacttatct
     8761 ttgccaggat atagtgttga tgcaatggtg agaaatcttg agaatgctgg aatgccttct
     8821 gaaaaaatcc ttctcggtat accattttat ggaagattag gtgccactat aacaagaact
     8881 tatgatgagc ttagaaggga ttatataaat aaaaatggat atgaatatag atttgataat
     8941 actgctcaag ttccgtattt agttaaggat ggagattttg caatgtcata tgatgatgct
     9001 ttatcaatat tcttaaaaac tcaatatgtt cttagaaatt gtctaggtgg tgtattctca
     9061 tggacatcaa cttatgacca agcaaatata ctggctagaa ccatgtctat tggtataaat
     9121 gaccctgaag tattaaaaga agaacttgaa ggtatttatg ggcaattcta atagaatgta
     9181 ggaagatttt atagttatat aaaaagcaga ttttattata ttaaataagt tgtatctaa
//
LOCUS       DAMKPG010000124         9180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMKPG010000124 DAMKPG010000000
VERSION     DAMKPG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.129"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            125..1753
                     /gene="aspD"
                     /locus_tag="PC621_003246"
     CDS             125..1753
                     /gene="aspD"
                     /locus_tag="PC621_003246"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF4110377.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1874..2869
                     /locus_tag="PC621_003247"
     CDS             1874..2869
                     /locus_tag="PC621_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF4110378.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3027..3695)
                     /locus_tag="PC621_003248"
     CDS             complement(3027..3695)
                     /locus_tag="PC621_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF4110379.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(3794..4106)
                     /locus_tag="PC621_003249"
                     /pseudo
     CDS             complement(3794..4106)
                     /locus_tag="PC621_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4533..6953)
                     /locus_tag="PC621_003250"
     CDS             complement(4533..6953)
                     /locus_tag="PC621_003250"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF4110380.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7307..7657)
                     /gene="rsfS"
                     /locus_tag="PC621_003251"
     CDS             complement(7307..7657)
                     /gene="rsfS"
                     /locus_tag="PC621_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF4110381.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(7774..8346)
                     /gene="yqeK"
                     /locus_tag="PC621_003252"
     CDS             complement(7774..8346)
                     /gene="yqeK"
                     /locus_tag="PC621_003252"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF4110382.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8347..9036)
                     /locus_tag="PC621_003253"
     CDS             complement(8347..9036)
                     /locus_tag="PC621_003253"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF4110383.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3020 a         1547 c         1214 g         3399 t
ORIGIN      
        1 ttattatttt aatttcatac tttacttttt ctataaacct attataatta aagtataatc
       61 atttttgcaa cttgtttatt ttattataaa aatgtgaaac ttattacata aggaggaatt
      121 taacatgaaa aatcatactt ataaagaaat caaaaacata tatggaaaaa taagcccttt
      181 tgaatttaag gacaaattaa tagatattgc aaaatacact gcaaaagaaa ataacagaga
      241 attacttgat gcaggtcgtg gaaatccaaa ctggacttgt tcaactgcta gagaagcctt
      301 ttttactttt ggtcatttcg ctattactga aactcgttct aattgggatt taggtcattt
      361 ggctggaatg cctcaaaaaa agggtattaa agaaagattt tttaaattta taaatgaaaa
      421 tatagatatg cctggagcat atttagctag agatattata aactttggta taaatgaact
      481 tggatttgat ggagatgaat ttgttcatga actagctgat ggaattattg gtgataacta
      541 tccacttcca gatagaatgt tacctcatat ggaaaaaata gtacacgatt acctagttca
      601 agaaatgaaa tatgatatat caggtaaata tggtgatgta gaaatttttg ctgttgaagg
      661 tgctacagct gctatgtgct atatatttga ttcattaatg gcaaatgaac ttctaaaaaa
      721 aggagataca atagccttaa tgactccaat atttacacct tatcttgaaa tacctaacct
      781 tcctcgttac gactttaaag tggtaaatat aaatgctaat gaagttgatg aaaaaggtgc
      841 tcatacatgg caatatacaa aaaaagaatt agagaaactt cgtgacaaat ctattaaagc
      901 tttattcgtt gttaatccta ataatcctgc ttctatagct atggatgaaa tatcttgtaa
      961 taatctaata gatgtaattg aaaattataa taaagattta atgataatat cagatgatgt
     1021 atatggaact tttgtagaag atttttcatc cttaatgtca aaactacctt acaacactgt
     1081 tggagtatat tcttattcaa aatattttgg agttacagga tggagacttg gtacatttgc
     1141 ccttcataag aaaaatgttt ttgataagaa aataaatgat ttaactggag aattaaaaaa
     1201 atcagtagat aagcgttaca gtgatatgag tcttaaccct tctagtcttt catttatgga
     1261 aagagttgtt gctgatagca gacttgttgc tctcaaccat actgctggtc tttctactcc
     1321 acagcaagta caaatggcgt ttttctctgc ttttgcattg attgacaaag tagatgccta
     1381 caaaaaactc aatatgagta tctgccatag acgtcaaaaa ctattatttg aagctttaga
     1441 gcttccaatt aatgaaaaca aaaacaatgc tgcatattac actcaatttg atattgaaga
     1501 atgggctaaa ttaaactacg gagaagactt atttaaattt ataagtaaaa ctgcatctcc
     1561 agtagatgtt ctttataaat tagctaatga ctattcagta gttttactta gtggaaatgg
     1621 attctatggc cctgaatggt caattagaat atctctagca aatctatatg atgaagctta
     1681 tactaagata ggaaaagcca taagagagat attaaatggt tatatcgttg aatggcaaaa
     1741 aattaagaaa taattatatt acactgaaat atctaaatta tatgaataaa agagtttatg
     1801 tagaccttaa ctactttttt tgatataatc ctatcggaat tataaatttt atttatgaaa
     1861 ggcagagaat cgtatgagta ttaagaaaaa agttgccata gtttttactg gtggaactat
     1921 atctatgaca gtagatgata aagtcggagc agctatacca actttatcag gtgaacaaat
     1981 actatcaatg gttaccaaca ttgataaagt tgcagatgtt gaagttttta attttgatga
     2041 aattccagga cctcacataa caccttatag aatgatggaa ttgaaaaatt atgtaaatga
     2101 tttattagca agaaaagata ttacaggtgt tgtaataaca catggtacag atagtttaga
     2161 ggaaacagct tattttctag accttacaat tgacaacatc aaacctgtaa tagtcacagg
     2221 tgctatgaga agtagttctg aacttggata tgatggttca agtaatttat ctgcatcagt
     2281 ttgtactgca gtatccaaag atgctatggg aaaaggagtc cttgtagttt taaacaatga
     2341 agtattatta gcttctgaag ctactaaaac aaatactctt tctctaaata catttaaatc
     2401 tctaactagt ggtccattag gaataattga ctgcaacgaa cttgtcctta caagagatat
     2461 tgtaaataga acaattatag atactgataa ggtagaatct aaagttgcct tatttaaagc
     2521 atatgtagga gaaaatgctg actttataag atttgctgtt gatagtggtt ataaaggtat
     2581 agttattgag gcaatgggta taggtaatat tcctcctcaa atgctttctg gagttgaata
     2641 tgctagagaa aaaggacttc ctgtagttat agtttctaga tgtcattcag gtagagtatt
     2701 cgatagttat ggttactttg gttctggtag agatttaaaa aatcttggtt gtatatttgg
     2761 tggggacttg ccaggtcaaa aagctagaat aaaacttatc ttagcacttg gaaaaactga
     2821 taaccttgat gaaatcaaag atttctttga aaaaggaatc tattgctaat tctaataact
     2881 tctatttaat tatttagttc agtttacaag aaaatacaca caaaccataa aatgagatgt
     2941 tgacaacata acagacagta tgctcctgta aaaggtaaca tactatctgt tatattgtct
     3001 ggattcccaa attagctatt tggaacttaa ctaaaaaatc ctaatataag ggcaactatt
     3061 ataatggcaa tcggaataca tactgtgtaa tcctgttcca tagagtcatc atctgaatgt
     3121 agttttacaa ttcgcaattt agcaatcagt ttgaagcaga aatagcctat tattgttcca
     3181 attacattcg tgagtaagtc gtctatatcg gttgctctgt gtaacgtaaa aagctgaata
     3241 gtttcgataa agagagataa tccaaaacca atcaagaatg tatttctcaa ccgttcaaag
     3301 gttttgctga taagcggaca taaaaaacct aatggaacaa aaagtaaaat attcaaaata
     3361 aaactcaaac taaatccatc actaaaagga ataagattta tgtttggatt aaagaacgtt
     3421 tcccctaact gtgacagtct tatatattca ctgaatgtag ggataccaac aatatgcgat
     3481 agtgttaaac ataagtagta atacattgcc agtgataaaa tagatgtttt tacctttgga
     3541 atatcactat ttttcttgaa tattacaact aagaaaatac caatcaagat aattcccaac
     3601 aagaaaatag gattaattaa caataaatct ttgatagctt caaacagact gagttgaatt
     3661 tctacacttg attgattatt tgatacagtc tccatttgta cacaccccct cataacattt
     3721 caattatttc taaaaaacaa aatagaagat aaatcttaaa atcatcctat caaaattttt
     3781 aaatttgcct ttatcagttt ataccaccag ttaattcctg tcttacttct gtatggtatt
     3841 cttgggattc tttcatcaaa ttagacaaaa aaatggttac catatcaata aactggttac
     3901 gctccatatc tcctgaagca aacatcccct gagaataaat ccgtatcagg aatctccaca
     3961 tctactgcac ctatcacaag tcatttgata acatcctcaa acaatcccaa cttggatata
     4021 tcagcaacac ccattagaat actactgctg accatccttg ttttcctggt gataatttta
     4081 atcaactggg aagttgaaac gcctatcttt tttgcaagtt cctttttaga aacatggtag
     4141 ttattatatg aatctgaatc tcgttgtctg ggtgtttaag ataaagcata gatacgcacc
     4201 ttttttgcct gttttcatta tataaaaaaa attttaaaaa tacaataatt atattttttt
     4261 cgtttaagct tagagcagtt ccttgatgaa caacacttta gaaactgaat aatttacata
     4321 ccacttaata cattaacacg atttaacatt aattataata atatttaaaa caacaattag
     4381 agtgtataag aatgaacttt ttagttcttt aatcattttt ttgtataaaa tcaaatctat
     4441 ttttgtaatt tcaacaatga aaaaaggctt atctctattt tgagacagcc tcatttattt
     4501 tataaattta tatttttgtt catatttttg agttatttaa caactatatt tactaatttt
     4561 ttaggtacag ctacaacttt aactatagtt ttaccttcaa ctaatccttt tattttttca
     4621 tcttctaaag ctaccttttc catctcatct ttagatatat ttgagttaac agttaatttt
     4681 cctctaactt ttccattaac ttgtacaaca acttctattt catcttttac taaagctttt
     4741 tcgtcatatt taggccagtc tatatcaaat acacttcctt ctttacctat catagtccat
     4801 agttcttcac ctaaatgagg ggcaaatggt gcaattatag ttactatagt ttgtacacct
     4861 tcttttataa ctgcttcgtt tatattatct aactctttat atttatacat atcatttatt
     4921 aattccataa gagctgatat agcagtatta aatccgaatt tttcacttaa atcagcagta
     4981 acttttttaa gtgtagagtg tatagtatat ctcatatcct tatcttggga atttaattct
     5041 ccaaactcta catccttttt aaccacatct gctaactcgt caaccaatct ataaacccta
     5101 tttaagaatc taaagcatcc atcaacacct tgctctgacc agtctaaatc tctttctggt
     5161 ggtgctgcaa ataatacgaa aagtcttgca gtatctgctc catactcatc tattatatcc
     5221 ataggagata ctgtattccc tttagactta ctcattttag aaccatccat aagtaccatt
     5281 ccttgagtaa gtaagttttt aaatggctca ttgaagtcta ccattcccat actcttaaat
     5341 gcctttacaa accatcttgc ataaagcaaa tgcattatag catgttctac tccacctata
     5401 tattggtcta ctggatgcca tctattaact aattccttac taaatggttc attttcatta
     5461 ttgctatcca catatcttaa gaaataccaa gaagaatcaa cgaatgtatc catagtatca
     5521 acttcccttc tagctggttt tccacagtga ggacaagttg ttgacataaa ttgctttgaa
     5581 gtcgtaagtg gagattctcc ctttccagta aattcaacat ctgttggtaa aagaactggt
     5641 aaatcttcct ttttaactgg aactattcca cattcatcac aataaaccac aggtatagga
     5701 caaccccagt atctttgtct tgaaactaac cagtctctaa gtctataatt aattgttctc
     5761 tttcctctac cttctttttc taacttatcg attattcctt caaatccttc tgaagcatct
     5821 aatccattaa attcttctga atttatcatt ttgttatctt ctgttattac atcaattatg
     5881 tctaaattat atttttcagc aaaatctcta tctctttcat catgtgctgg aactgccatt
     5941 atagcacccg ttccatactc aactaataca tagttagcta tccatagagg aactttcttg
     6001 ccatttaaag gatttataac atatctacct ataaacattc cttctttttc tacatcagct
     6061 gctgttcttt ctatttctgt catagtatgc attttttgaa caaaagcttc aacatctgct
     6121 tcatattcag tccctgcaac caattcttta actaattcat gctctggagc aagtaccata
     6181 taagtaactc cataagttgt gtctggtcta gttgtaaata ctgtcataga tttatcagtt
     6241 ccatctatat caaaaactaa atctgctcct gtacttttac ctatccaatt tttttgcatt
     6301 gttttaactt tttctggcca tccatctaat gtatctaagt cattaagtaa ttcttctgca
     6361 aattcagtag tcttaaaata ccattgttct aaatctttct ttaatactgt tgctttacat
     6421 ctctcacatg ctccttgaac aacttgctca tttgcaagaa cagtctcaca agaaggacac
     6481 cagttaacat atgatttttt cttatatgca agcccatgct ctaaaaactt caaaaatatt
     6541 tcttgtgtaa atctatagta ttctggagtt gaagttgcaa cttctttatc ccaatcataa
     6601 ctaataccta ataaatttaa ttgctctttc atttcttcta tattttccat tgtccattta
     6661 tgcggatgta ttccatgttt tatagctgca ttttcagctg gtaatccaaa agaatcccat
     6721 cccattggat gaagtacatt atatccttcc atttttttga atcttgcgac aacatcacct
     6781 atagaataat ttctaacatg tcccatgtgt atttttccag atggataagg gaacatttct
     6841 aatgtgtaat aattaggttt ttcagtttgt gcagtatcca ttttatactg attattatct
     6901 ttccaaattt tttgccattt cgattctact tctttaaaat tatatacgct catattttgc
     6961 ctcctatttt taattgtgta taactacctt atttagtatt atttacttat aatttccaaa
     7021 taattctttg aaaataaaaa atcctcgtca ctaaacttac gtttagggac gaggataaat
     7081 cttcgcggta ccaccctaat tagcctacta catatataga ctctcttaga tagcttttaa
     7141 tccagccatg gagaatagat tttaatctag taagttcaaa gttatataat attagttctc
     7201 accttccact aactctctga aaaaatataa tcttttactg ctctagtcta ttcaatttaa
     7261 tataattttt gaaattttct aattataata acatatatta tcatgtttat gctaatgtgt
     7321 caatatctat ataaggtgca tctttccata atttttctaa attataaaac cctctatttt
     7381 cctcattaaa tacatgaacc attatgtctc cataatcaag gagtacccaa gtttgagttc
     7441 cttgtccttc tttacctctt ggctctaatc ctagttttgc taattcatct tccacattgt
     7501 cagcaatagc ttttacctgt ctctgagaag atgctgtagt aataataaaa taatcacaca
     7561 aactagaaac ttttcctata tttattataa ctgtatcttg acccagttta tcttctatgg
     7621 catcatatgc tatttttgtc atttgttcca ctgtcatata aaaattcctc actttcctaa
     7681 ttaaaataca cttagttatt gtaacatatt aatacgattt tgtaacatag tcaaatttaa
     7741 atttattaat atgtttgcct ttatatagct tttttataat aacttttctt taattagata
     7801 gtttcttgcc ataactgttc ttggatgtat ctcttctttt ttattgatga caaacattaa
     7861 agtattatta aaagaagtta aaagtgcttc atcaagcttc cctccatatg ctaattctct
     7921 taatgtatct acaactggaa attttcttcc ttcttcaatt aaatcagcta tgtaaattat
     7981 tttttctaaa agagacatgt cctctctccc tgttgtatga tatttgattg catttataat
     8041 atcttcatct tctacttcaa attcatactc gcatatgtac gccccaatta cactatgaga
     8101 caaggaacga tttccatcct ctattgggtc taaatatatt tcatacttat ttacataatc
     8161 ctctatttcc ttgtcactta aatactttgc acaatcatga atcattcctg ctaagtaagc
     8221 tttttctttg tcacatccat aaatctcaca taatttttca gcacattttg caacatttct
     8281 tgaatggcta agcctcccca ctggtagcat ttgatttagt ctttggttaa tattttctaa
     8341 gttcatttaa tcatctccat attgatatag tttattttca tataaataat tttctacatt
     8401 ttcgggtact aagtaacgta ttgacttgcc ttcatttagc tgttccctta tatatgtgga
     8461 cgatatatcc agtgatggaa cataaactgt tataatgtca gcattatact tcttagttag
     8521 tttttctata gtctcttgtg accttaacaa actaattcct ggtctagttg cggctataaa
     8581 agtagctaac tcaaagtttt tctttacatc tttccactct tcaacactac aaattgcatc
     8641 tgcacctgtt ataaaataaa tgtctgcatt tttgtatttt ttctttaagt attttaatgt
     8701 atctattgta taagtccttt tctttctttc gatttctact tttgaaacta aaaaatcttc
     8761 gttgctttca gttgctagaa gtgtcatatt atatctatca tacttatctg tagtgataca
     8821 taatttatgt ggtggatttc ctgatggtat aaagattatt ttgtcaatgt catacttgtc
     8881 tctaataaat tctgctgttg ctaaatgagc atagtgtata gggtcaaaag tacctcccaa
     8941 tatacctatt ttgattttgc ctttatggcg attaaatttc tctagttttt cagtattttt
     9001 caactctgcc atcttaacta gattttcact tctcatattt tgtctccttt taatgttata
     9061 atactattgt accattattt tttatttctt aaagcttata atttgttttt ttgggtatat
     9121 aattatattt atatgctaaa aaacacttta aaaaataatt atttaacatg attctgcttt
//
LOCUS       DAMKPG010000125         8968 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMKPG010000125 DAMKPG010000000
VERSION     DAMKPG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.130"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(384..1280)
                     /locus_tag="PC621_003254"
     CDS             complement(384..1280)
                     /locus_tag="PC621_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF4110384.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1422..2849
                     /locus_tag="PC621_003255"
     CDS             1422..2849
                     /locus_tag="PC621_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4110385.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(3046..5640)
                     /gene="clpB"
                     /locus_tag="PC621_003256"
     CDS             complement(3046..5640)
                     /gene="clpB"
                     /locus_tag="PC621_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF4110386.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5677..6273)
                     /locus_tag="PC621_003257"
     CDS             complement(5677..6273)
                     /locus_tag="PC621_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110387.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6290..6694)
                     /locus_tag="PC621_003258"
     CDS             complement(6290..6694)
                     /locus_tag="PC621_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF4110388.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            7061..7435
                     /locus_tag="PC621_003259"
     CDS             7061..7435
                     /locus_tag="PC621_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4110389.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7432..8295
                     /locus_tag="PC621_003260"
     CDS             7432..8295
                     /locus_tag="PC621_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110390.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRNPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            8295..8933
                     /locus_tag="PC621_003261"
     CDS             8295..8933
                     /locus_tag="PC621_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF4110391.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         3001 a         1319 c         1035 g         3613 t
ORIGIN      
        1 aatatataat ttttttatat ttatttgact ttcttttaat tctttaggat ggatttaaat
       61 tttctataaa aatcatacta ttttagctgt ttacttgctt atttgatagt tttatttctt
      121 tgattaaata tctttcagaa tctaagccag atgtctcaaa aaaactgatt tataatataa
      181 tcattttttc tgctattctt atttttccaa ttttagtttc atattaaaat atacttttca
      241 tagacactac attctttcat actcattttc acatttaact atacaagtag tcttactcat
      301 ttatgtacta tactttatta atatttatta agaaaaactc aaagatttaa aaatatatat
      361 tttctataaa gtatatttat gaattatttc atatcttctt ttaaaataac attactaact
      421 tctctatgtt catttctata cttagttgga gatatattat aattcctttt aaataagcga
      481 ctaaaataat ttatgctatc aaagccacat ctcatagcaa tttctgtcat atttaaatct
      541 gtttccttta aataatatat agctttatca agcctgattc catttataaa atcgattgta
      601 gtcttacctg ttatttgctt aaatgttcta caaaagtgat gtgcactcaa gttcacacga
      661 ctagcaagtt cttttaatgt aattttatcc atatattttt cttctatcaa gtcaaagata
      721 cattcaaatt tttctaaaga atactttctt tttataaatt catcttcaga atatatttta
      781 tcaatatgac ttcttaataa gagtactatt aattgataga tattagcttt tatagataac
      841 tcatatccaa cttctttatt gtgatattca tcaagtatag ttgtaataca ttttaaaatc
      901 agaaaatcat tttgaattaa attattaaat acaatatgat ttaaagccaa tggtgctaag
      961 tacttagttt gtaacaaatc aacttgatta ctaaaaagga atgtgggctc aatacgaata
     1021 ctatagaatt ttaaattatc tgataagctt tctaaataat gtaattcatt actgtttgct
     1081 attacaatat ttccaggcac taccttaaaa ttatccaatc cacattcaag atatgctata
     1141 ccatcaataa aataatatat ttgtagatgc tcatgccaat gtcttttaaa gtttgctcct
     1201 tttttaatgt catcctttaa aaaaatatct ataaagtttt cttgaccttt cctattaaaa
     1261 tatgtatttt tatccttcat tattcccccc tcatcaaaat agtgctaatt ataagcaata
     1321 tagttattgt attcattatt tcatatcgct attatataaa atgtaactat cattgtaaac
     1381 attaacataa tgccagtcat tataataagg aggtaataaa aatgacaaac tcttttaaaa
     1441 aagatgataa tagaaaaaaa atcacagttt tatttgtagt cattgccatg acttttatgg
     1501 caacattaga ttcgagtatt ataaatgttg ctctcccagt tcttgcaagc aaactaaatg
     1561 tatctctagc atctattgaa tgggtaatag caagttactc aattattatt tgttcaacac
     1621 tattattttt tggaagactt ggagatatta ttggtaaatc aagagtattc caagtaggta
     1681 ctattctctt tacatcagct tcattactat gtggattaag taattcttta actttactta
     1741 tagtttgccg ctttattcaa ggaataggcg cttctgccta catggcaaac aatcatggaa
     1801 ttatcacaga actatttcca aaagaaagca gaggaaaagc cctagggatt ctagttactg
     1861 cagttgctat tggaaatatg gttggaccat ctgtgggagg atttattttg tcaatatttg
     1921 attggaatgt tatatttttt ataaatattc caatcggtct tattgtaatc tttttaaata
     1981 caaaatttct tcctaatagt aaaaaatctt cagaaaatat ggataaaact ggtgctatac
     2041 ttcaattttt aggaacaaca ttatttttta gtgctctaat tagtgctcaa caaacaggat
     2101 tactaaatcc atatatatta attgcactat tattatcaat tatttttatt atattatttt
     2161 tgatattaga aaaaaaacat cctcaaccgc ttcttgatct tgaaattttt agaaatttta
     2221 aattttcatt gaatttaatt tgtgctctta catcattcat ttgtattgca tcatcaagta
     2281 ttttaattcc attttactta caaagtacta tgaaacttcc ccctattcaa gcaggtttat
     2341 ttatgatact atcacctttg attttagcca ttttttcacc tatatttggg aatatatcag
     2401 ataaaatcaa gtcagaaaaa attattttaa ttggtcttct cgttatgagt tttggattct
     2461 ttctaatgtc aagattgaaa gaaagttctg cccttatttt atttgtaatt tatatatcga
     2521 taatatcaat aggacaagct atatttcaac ctgctaacaa tgctttgata atgtcaagct
     2581 gttctagaag taagctagga gttgtaggaa gcataaattc ccttgtaaga aatttaggac
     2641 aagttatagg tattactatt tctacaacac ttttatataa ttttatgagt attaaagctg
     2701 gatatagggt caatgattat gtgattaata atgataaaat atttgtattt ggaatgagaa
     2761 atgtttatat tattgttaca cttgtttgct taataggtgc aatattaatt ggcttttatt
     2821 tatttaaata taataaaaat gaacagtagt tttttataat tatattcaaa ttatgattat
     2881 attataagaa acttactaaa atacttcctt tgagttaaat ttaatttgga catattgact
     2941 taaaaatgtt ttattaattt gataaaatat ttcttttcct atagtttata aataaaaatc
     3001 agctgagcaa aatatttagt atattatgct caactgattc ttttattatt taactaaaac
     3061 ttcaatattt tcatcttttc catctataac tatagtacat ccattataca catctccagc
     3121 aataagtttc tttgcaatta tagtttctaa tgtatttcct atatatctct taagaggtct
     3181 tgctccatat acagggtcat atccttctct caccataaca tcttttgctg aatttgtaac
     3241 ttcaatagta atatcttttt cttttaatct attttttaaa tctttcataa atatatctat
     3301 aattttcttg atattttctt ggtctaaagg cttaaacatt ataatatcat ctactctgtt
     3361 taaaaactca ggtttaaatc tatgcttcat ttcattcatt actaaattgt tagtagtctc
     3421 tgttatattt cctcctgctt ctaatagata attacttcct atattagaag tcattattat
     3481 aagagtattt ttaaagtcta ctgtcttacc tttattatca gtaagtctac catcatctaa
     3541 tatctgtaag aacatgttaa atacatcctc atgtgctttt tctatctcat caaatagtat
     3601 tacagaataa ggagcacgtc taactgcttc tgtaagttga ccaccctctt catatcccac
     3661 gtaccctgga ggaggtccaa ctagtcttga tacagcatgt ttttccatgt attcagacat
     3721 atctattctt attatattat cgtcactatc gaataaattt ctagcaagag tttttgcaag
     3781 ttcagtttta ccaacacctg tagggcctaa gaaaataaat gaaccaattg gttttctttc
     3841 atcttttaat cctgctcttg cacgtattac tgcatttgaa actgctgtaa ctgcttcatc
     3901 ttgtccaata actcttttat gaagttcatc ttctagttta agtaattttt ctctttctcc
     3961 ttcaactagt ttagttactg gtatacctgt ccactttgat acaatgctag atatttcttc
     4021 ttcagtaact tcttctttta aaagagaatt ttcagaacca tcattcatat tttcttcgta
     4081 ctgctttatt tgttcttcta acttaggtat ttcaccatat ttaacctctg ctgctttgtt
     4141 gaaatcatat tctctttcat acttttcagc tttaccttta gcttcgtcta attgagcctt
     4201 taagttttta atatctaata tttgactctt ttctttttca tattttgctg tcatttcatc
     4261 atttttagat tttaactctg ctatttcttt ttgaatatca tccaatcttg atttgctttt
     4321 atcatcattt tctttaagta atgcttctct ttcagtttct aatgtaaata actttcttct
     4381 aacaacatct aattctgttg gcaatgaatc tatttcacta cgaatcatag ctccagcttc
     4441 atcaattaaa tcaattgctt tatctggtaa aaatctatct tgtatatatc tatgagaaag
     4501 ttttgctgct gctacaatag cattatcatg tattcttact ccatgatgta tctcaaatct
     4561 ttcttttaaa cctctaagta tagatattgt gtcttcaaca gtaggttctt ctgcaattac
     4621 aggctgaaaa cgtctttcaa gagccttatc tttttctata tactttctat attcatcaaa
     4681 agtagttgca ccaatacagt tcaattcacc tcttgcaagc ataggtttaa taagattacc
     4741 tgcatccatt gaaccttctg ttttaccagc tccaacgata gtatgaatct catctataaa
     4801 cagtataatc tttccatcag aactttgaac ttcttttaaa actgccttta atctttcttc
     4861 aaattcacct ctatattttg caccagcaat taaagctccc atatcaagtg agaatacaat
     4921 cttatctttt aaaccttctg ggacatctcc tcttactatt ctctctgcta acccttctac
     4981 aattgcagtt ttacctacac ctggctcacc aatcaataca ggattatttt ttgttcttct
     5041 tgataaaatt cttataatcc ttcttatttc ttcatctctc ccaattacag ggtctaattt
     5101 atttttcttg gctaaatcta ctaagtttgt tccatatcta gcaagtgcat catatgtgcc
     5161 ttctgggtct tgtgtttcta ctctttgact acctctcact tgtgataaaa tatttaaaaa
     5221 gtcattctta tttatattat attgttttaa tattttacca acagctgatt ttgattctgt
     5281 ttccatcatt gcaagcataa catgctctac acttatatat gaatctttaa attctttaga
     5341 aatactctct gcctttataa gcacctcatt tatttttcta gtagctgata ctccttgtga
     5401 gtcagcacct tctccatata cttttggaag cttatcaatt tcaaagttta cagtatttct
     5461 caaagaatca attgatatat tcattttttc gattatattt ggtatcaatc catcctcttg
     5521 attaataagt gctgagaaaa gatgaattac atctacttgt tggttatgat tttttactgc
     5581 ttcgttataa gcctcattta aacttttttg aactcttaaa gtcatttttt ctacgtccat
     5641 ataaatccct ccattttaat attatatcca aataaattaa attctctcat ttataattat
     5701 tgattttcta agctctaaaa gactatcaag taaattctcg tttttttgta tatcgaaact
     5761 caatgttaaa ttaaatgttc catctgaatt aaaaaaatcg aatacttctt ttgattttgc
     5821 tattaaatac aagtcttcat ctataagtct tttttcacct attaataaat actgacattt
     5881 atctataaaa ctctttttaa aatcaatagt gtatagattt tctaaaatat tatattgata
     5941 aatagcaata ttattaaaca cttttaatat gtttgaattt acaaaattgt aaatttgatt
     6001 attgttatac agtgatattt gttcgtatat atattcccta aataaaggat gaatgttttg
     6061 cttaagtttt aaagtaactt cagctggtat ttcaaaataa agcttccagt acttagagtt
     6121 atttttttct aaaataaaag ctactttgtt caaactggta gaactaatct cctttagtgt
     6181 acaattaaaa ttatagtgtc tcaaactagt tttttcattc tcaaatacat agtcaaacca
     6241 ctgtagaaca tttctatcaa gttttaaact catataaaaa ccttcttcct tatttactaa
     6301 ccttattagg tctttttatc tgattatatt ctgctaataa actttcttta tcagctctac
     6361 tacctttata tgctatatgt ggatttatca tatactcacc atttttcact ctttgtaaaa
     6421 aatccatttt tttcaactcc gtaaacaatg aagataaaga agtttttgga tattcaaatt
     6481 cacaagataa ttcatataat gtttttctaa tcttattatt tttatccata gagtttatta
     6541 attttaatgg aattaaatcc ctcttagtat acattccttc tactacttta atataattct
     6601 taaaacaaat attcatatac ttacttttat tcataactaa acttttgctc tctgtatatt
     6661 cctgatttaa atcctttttt aatgtcttat ccaaattaaa tatctcttct cactctcctt
     6721 atctctaatt gataatataa atatattaca aaaaaatatg tatgtcaaaa ctgaaaatgc
     6781 aaaaagttta ccttataagt aaacttttgt ttagctataa gctaaacttt attatttttt
     6841 taatttagta tttccaattt attggcagga tttttgaata atatttataa attttttaaa
     6901 aaaatttaaa tctatttaat atggcagcct ttcataaaat acattacatt tattaaatgt
     6961 ataatattgg taattagttt tttctaaaat cagttgacat attatatata tatttatata
     7021 atgtgtatat actatatata caatttaagg aggtgactta ttgaatatag ttattagtaa
     7081 ttcgagtggg aagccaatat atgaacagat tactacacaa attaaaagta tgattataag
     7141 cggagagtta ccagaaggct cagctcttcc aagtatgaga ctattggcaa aggaacttag
     7201 aataagtgta attactacaa aacgagcata tagcgattta gaacgagacg gatttattga
     7261 gacagttact ggtaaaggca gttttgtatc tagtaaaaac atggattttg tcaaagaaga
     7321 acaatttcgt ttaattgaag aatacctaca aaaagcagtt gatacagcaa aaagtagtga
     7381 tattacatat gacgaattaa aagatatttt atttttatta tataaaggag aatgataagt
     7441 atggatgcaa taactataaa aaatttaaat aaaacttata aagattttag cctacaagat
     7501 atatcttttt cagtaccaaa agggagtgta atgggatttg taggtgaaaa tggtgcagga
     7561 aaaacaacta cattaaaagc aattttaaat ttaatatctt atgatagtgg aaatatagaa
     7621 atatttggtc ttgatagtaa aaaaaatgaa aaagaaatta aagagcaaat tggggttgta
     7681 tttgagggaa gcaactttca tgaaaattta aacactgacc atgtttcaaa aatcatgagt
     7741 aaaatctata aaaattggaa tgacacatta tttaaagact acttaaaaaa attgagagta
     7801 ccagacaata aattaattaa ggagttttca aaggggaata agatgaaact tagtattgct
     7861 gtagccttat ctcataaacc aaaattatta atactagatg aggcaactag ttcacttgac
     7921 cctatcgttc gtgaagaaat actagatata tttttagatt ttatacaaga tgaagaacac
     7981 tctattatct tatcttcaca tattacaagt gatttagata agatagcaga ctatattaca
     8041 tttatacata aaggaaaaat tgtatttagt gaaaataagg atgaattaat tgatactatg
     8101 ggagttttaa aatgtaaacc tagtgatttt gataaccttt caagagagga ttattcatat
     8161 tatcgtaaaa gccaatttgg atatgaagta cttcttaaag ataaacatag atttatatca
     8221 agaaatccaa attgtattgt tgataatacg tctatagaag aaattatgtt attttatgtg
     8281 agaggtgata aataatgaaa ggtttaattt taaaagattt acttaatttg aaaggaaatg
     8341 taaagtttat acttttattc atcattatgt ttgggtttat gtcttcttta ggagatggaa
     8401 atgttaataa ctttataggt gttattatag ttttatgtac tacaatgatt gtttcaacat
     8461 tttcatatga tgatttaaat aaatgggata gttatgtact tacaatgcct attaatcgca
     8521 atgatattgt attaagtaaa tatttaacta tgttaatatt tagctttata ggtgttcttg
     8581 tttcacttat tgttagtgta actattggtt attttaaaaa tacacttata ttaaatgaaa
     8641 cactacttat taatgcacta atcttatcaa tttcagtatg ttttggtagc cttattctac
     8701 cacttattta taaatttggt acagaaagag ctcgtttatt aatgatttta tgctttttag
     8761 ttccaacact agctttatta gtttttaaaa gtattttaga aaatattagt tcacctattt
     8821 ctatagaaat tatacttaat acactagtat attctcttcc atttgttgct attttactat
     8881 ttgtgatttc atattttata tcatcaaaaa tctatagtaa aaaagaagta taaaatatac
     8941 tatacatgtc tctgtattta agtatttt
//
LOCUS       DAMKPG010000126         8922 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMKPG010000126 DAMKPG010000000
VERSION     DAMKPG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.131"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            230..1309
                     /locus_tag="PC621_003262"
     CDS             230..1309
                     /locus_tag="PC621_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF4110392.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="PC621_003263"
     CDS             1633..2001
                     /locus_tag="PC621_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF4110393.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="PC621_003264"
     CDS             2118..2921
                     /locus_tag="PC621_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110394.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="PC621_003265"
     CDS             3306..4196
                     /locus_tag="PC621_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4110395.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="PC621_003266"
     CDS             4659..5102
                     /locus_tag="PC621_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110396.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="PC621_003267"
     CDS             5274..5444
                     /locus_tag="PC621_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110397.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="PC621_003268"
     CDS             5995..6903
                     /locus_tag="PC621_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF4110398.1"
                     /translation="MTSENVGEVVTEDGYANPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            7083..7571
                     /locus_tag="PC621_003269"
     CDS             7083..7571
                     /locus_tag="PC621_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF4110399.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7657..8064
                     /locus_tag="PC621_003270"
     CDS             7657..8064
                     /locus_tag="PC621_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110400.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8065..8283
                     /locus_tag="PC621_003271"
     CDS             8065..8283
                     /locus_tag="PC621_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110401.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
BASE COUNT         3540 a          883 c         1551 g         2948 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaatcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcctctttta aatttaaatt
     6961 aatataaaac cttgttattt aactatagta gatacaaaga gatatataag ctaaggagat
     7021 gattcaaaat aaaagtttga actatctcct tcttatagaa aaacaaaggg gttgatttat
     7081 atatgaaatt ttatactata cagactttag aattttggaa caacaataaa aacaataaat
     7141 atctatcaaa tgattattct tttatattag aagactttga tattccatac aaatggatgt
     7201 atagtcaaat gacaaataga atatatgaat ttgatgatag tatgatatgg gtttggacga
     7261 agagacctga tttaagatgt agtggttatt tagaaaaagg aaaaaaaggt gttttacttg
     7321 aaatagaaat aagtgaagac aatgtgttat tatcagaatt tgatttatgg catttacctt
     7381 taatggatat tactgtagag ctttatgaag atgaacaaat tgaaaagaag gtaagttggg
     7441 aaagaatatt tgacttagaa ctgtgttcaa aattaaatga agttaatata gaagatttga
     7501 cattgcaagg agtgacatct aaagtgcctc tatctaatgt aaaattaata aaagaatttg
     7561 ttgcaaaata atatttttat tgaaatttat tcaaaaaagt tatatattta taacaagagg
     7621 tgttataaat acataacttt ttaaggggga ttttatatgt ctaaacaaca aactggtatt
     7681 aaatcaacac ttatttcaga aatattggga atttcttatt gtattggagc tacattagta
     7741 aataagtatt tttttaaagg aagtagtatt gaatatcttc ctataattat ttgggtatta
     7801 gctatgggag ctcagtgggt ctataataaa aagtatgatg atttaattga tgaggcatca
     7861 aaatgtatac tatcaagagt taatgatatt gcaataaaag tattgttttt ttctgtagct
     7921 atagtatcta tatttcttgt aactttggac ataaatgtgt caaatttaaa tataggaatg
     7981 tctttattag tcattttgtt tattcaatcc ttgttgaagt taattctatt tatatatttt
     8041 gacagaaagg ggatatacaa ttaggtgtca gtattactaa agacaaaaat taaatcctat
     8101 agagaaaaga atcttatgac acaaggtgaa cttgcaaagt cagtaggagt aagacgtgaa
     8161 actattgtac atttagaaaa tggtcgctac aatccttctt taaaactggc aatggatatt
     8221 gctaaggtat ttgatacaac tgtagaagag ttgtttgaat ttattgaaga aaataatatt
     8281 taaatttcat gtattactta tgcattagag aatgaattaa tagataaaaa attcaagttt
     8341 atgtattaga gaatgaatta attataggta aaaaaccaag cttatagatt agagaataaa
     8401 tgacttatag atgaaaaacc aagctcatag attaaagaat gaatgattta tagatgaaaa
     8461 agaaagctta tagattagag aatgaattaa ttataggtaa aaaacaagct aatagattaa
     8521 aaaatgaatg atttatagat taagaagtaa gcttatagat tagagaataa atgacttata
     8581 aataaaaaga aagcttatag attagagaat gaattaatta taggtaaaaa acaagcttat
     8641 agattagaga ataaatgact tataaataaa aaccaagctt atagattaga gaatgaatta
     8701 attataaata aaaaccaagc ttatagataa gaagtccatg cttataagcc agaaaatgaa
     8761 tactttaata gattaaaaaa tacatatttg tgaattatag aatgaataac tccataaact
     8821 agaaaattta taataatatc tatatgaaaa ttagaattat tttatcacaa aaaaattaag
     8881 ttaaataaat atgaaattac ttccaaatat acttccaaaa ac
//
LOCUS       DAMKPG010000127         8696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMKPG010000127 DAMKPG010000000
VERSION     DAMKPG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.132"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(148..480)
                     /locus_tag="PC621_003272"
     CDS             complement(148..480)
                     /locus_tag="PC621_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF4110402.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(724..1365)
                     /locus_tag="PC621_003273"
     CDS             complement(724..1365)
                     /locus_tag="PC621_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF4110403.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1346..1639)
                     /locus_tag="PC621_003274"
     CDS             complement(1346..1639)
                     /locus_tag="PC621_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4110404.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2033..2092
                     /locus_tag="PC621_003275"
     CDS             2033..2092
                     /locus_tag="PC621_003275"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110405.1"
                     /translation="MIEYIYYYNNKRIKKINLA"
     gene            complement(2608..3276)
                     /locus_tag="PC621_003276"
     CDS             complement(2608..3276)
                     /locus_tag="PC621_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF4110406.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(3221..3361)
                     /locus_tag="PC621_003277"
     CDS             complement(3221..3361)
                     /locus_tag="PC621_003277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110407.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(3420..5075)
                     /locus_tag="PC621_003278"
     CDS             complement(3420..5075)
                     /locus_tag="PC621_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110408.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVDSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(5304..5672)
                     /locus_tag="PC621_003279"
     CDS             complement(5304..5672)
                     /locus_tag="PC621_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110409.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            5821..6615
                     /locus_tag="PC621_003280"
     CDS             5821..6615
                     /locus_tag="PC621_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HDF4110410.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(6768..7196)
                     /locus_tag="PC621_003281"
     CDS             complement(6768..7196)
                     /locus_tag="PC621_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF4110411.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(7672..8610)
                     /locus_tag="PC621_003282"
     CDS             complement(7672..8610)
                     /locus_tag="PC621_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF4110412.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3022 a         1416 c          839 g         3419 t
ORIGIN      
        1 atttaaaact actatattat ttaaaaattt atatttatat atgcaattct aaaaacttaa
       61 taagaagtga acttagaaaa taaagcttaa tttcaaagac ttaaatagaa tattattatt
      121 agtctaaata aactacgtgt atcaaaatta tatattaaat tcttctaaaa tttttattgc
      181 aaatcttgcg tttggatatg taatagaccc gccaccaatt acacctattt ttatagcttc
      241 tataatttca tctttacttg ctccagaatt aatacaaccc tcaatatgat aacaaataca
      301 ctcatcacat cggcttaaaa ttgaaatagc tagtcccatt aattccttat tttttttact
      361 tatagcccct tctgaataag tcttatcatc aagacttcca aatactctaa aaaactcatc
      421 tgtttcactt aattttttat tgtaaaatcc cctatattct ttcatttctc tagtattcat
      481 cttctacctc ctatgttttt cttataagag tatcacactg tacttatatt ttcaatatta
      541 aatacaaatt ttcattttat gttcattagc ctaatttata ttattttttg aaatctcaat
      601 aagttatcat ataagtaaga ttttttcaga actaaaatag aaatcttagt aatttcttta
      661 ttatctatct cagttctgaa atatattatt cttatccaaa cattattata gtgattaatt
      721 atattagtta tttttacctg aaattttata catcaaataa gaatatataa atggtagtat
      781 tacaattaca aagattacaa agatattata aaaaggaata ttaataaatg cattaataat
      841 taataatatt ccaccaataa gccaaatcca tccagctaaa tggtgtgtct tattccaatt
      901 atcttcatca tcaagagtcc atggcaaaat tattcctact gagtaatttt gtttacattt
      961 aggaagatag tttccaaata aaataactat tattcctaaa attaatcctg tataaaaatt
     1021 agactccaaa ctaactcctg ttgaatataa aaccatcagt atttgtagaa ttactgaaag
     1081 tatagcaact ccccattttg ataaattttg tataacattt gaaaaattag cttttttagg
     1141 ctcattctca agtctaaacc atgtatacaa atggatacat agcattaaca atggtacacc
     1201 aaaagctgct ataacctttg gagcataatt atctggattt ccataattat caaaatgaat
     1261 tgcaacttca tttggtaatc tattataaaa atatattgat acaataaatg gtataaaaca
     1321 tattaaagta gataaaataa attgtttatt tatcgctttt ttcattatct ttttctccct
     1381 tctccataaa ttgtgaaaac caaagcataa cctcttcgaa aactgaaata tttatttcgt
     1441 agaaaataaa gttcttatac tttgtttcaa atatgagtcc tgcctttttt agttgagata
     1501 gatgataaga aattgtagca ttcgtcatat taaatttttc actaatttct cctgctgaca
     1561 ttttattctc cctcaacata accaatattt ctcttcttat agggtctgaa agagctttga
     1621 atgtttctgg aaaccccaac accatcacct cgctatttag aatttcatct aaatagaata
     1681 taacataaca tccattttat ttcaataact atttagaaaa atttctaaat agttatttcc
     1741 aaatatttat tgaaaatttt catattaaga ttctcaattt acttaagtcg cttttatata
     1801 atcttatttt atctaatttt tttaatttaa caaaagtgta ttgcaccaca attgttaaag
     1861 caaaaaccta atgactgata tacttatata tattacatat acttttaatt tcaaattaaa
     1921 agtagctaaa tccaatttaa aaagtagtta agtcctattt aaatagaaaa gaatgaaata
     1981 agaaatatac tgtataaaaa tgcataaaaa cctctactaa ttagatacaa ctataataga
     2041 atatatttat tattataata acaaaagaat taaaaaaatt aatttagcat aaccttgtta
     2101 aatatagatt tgaatatgaa tttaaagtta tgtaaaatta aaaatacagg aatgaaaatt
     2161 ccattcctgt ataaaatcta acttttgagt ctcaatacaa tcttattttg agacaatctt
     2221 ttttgtttta tattttctta tatttaaaat ataacgcata tcttatgcaa catattcata
     2281 tttttcttta tattctttca tcgcatcatt ccaagcgtct tgtactgtta cacctctttt
     2341 attagcaatt ttttgtgcca gttcaattat cttcatagct cttcttgata tgttcataaa
     2401 cattcctcct acaaattacc atatatttca tatatataaa tcaaggttat atttttatga
     2461 tatataatcc ttctattgta atattacaat ttatatgtgt attatatatg aactatatta
     2521 gaagataatt agaattttta taaagctata tgattagatt aaatataaaa gctttacaat
     2581 tacttataaa tttaaaaagt ctataaacta aactaatgtt tgatatacca attttacgac
     2641 cataaataat gccattacta aaaatattgg ttttatcatc tttgaacctt tctttatagc
     2701 aactttagca cctacataag ctcccattat catcactaat gcaaacacta tagcaatttt
     2761 ataatctact tgaccattta tcatgaataa taacaatgaa gttatattac ttgttaagtt
     2821 taatattttt gtatttgcag aagcatgtaa aaaatcatat ccatatattt ttataaatcc
     2881 aaaagtcaaa aaagttcctg tacctggacc aaaaaaacca tcataaaaac ccattataag
     2941 agccataagc atccctagtc ttaaattttt cttattaaca gcttcaaaat tatcttcttg
     3001 acctaaattt ttagaaataa aggtgtaaat agccactatt aaaatcataa ccacaaccaa
     3061 aacatttaat atctcttcac ttattgataa tacacatctc acacctagaa cactaccaat
     3121 aatagtaaat ggtactaatt tttttaaaag atcgttatta atttttccag actttgcata
     3181 tctataggca ctacttatac atcctgctga tgctgcaaat ttatttgtac caatggcaag
     3241 atgtactggc actccaattg ccataagaac aggcatgctt ataagtccct cctcctcctg
     3301 ctatagaatc tacaaaagca gctaaaaacc cccctataca taaaaaaata actgaaaaca
     3361 taaaattcct ccatcattct ctttcaattt ttcttacaat atatattata taatcttatt
     3421 taatacttat tatccagcat attctttcta tattcatttg gagttatact attaaacttc
     3481 ttaaatattt gtgaaaaata actactgtta ttaaaaccta ctttcttaca aatatcacta
     3541 atactcatgc ttctatctaa cagatatttt ttagattctt ctatcctaag aagatttata
     3601 tactcagtta ttgaaacttt atttctttca ttaaaaatag aactcaaata atttggactt
     3661 ataaaaaaaa catctgatat actattcaaa tttatatttt ctttataata tttttttata
     3721 tattcgttta tattttcaat tagatttcct ttatcttttc tccaaaatgc tctattctta
     3781 tcaatacact ctttggaaaa ttcaaataat acattccata aatcttcatc actgtctaca
     3841 agtgatattt ttttattata tttatttctt aaataagata taagttttaa atcctctaaa
     3901 tctctaagca ctgttctaga caatacaaat atcatttcct gaatagtatc tctaacatca
     3961 ttatatctta atttgtttgt atctattaga ttataaacac cttctaaatt catattttta
     4021 atttcttttt caatataaag ttctaattca aagggatatt cttttgaaga attattgaac
     4081 atatctatcg gattaataat atactcttct tctaataatc tactatttgc ttttttaagt
     4141 gcctcttcaa gtcttatatt ttcttttcta ctctttaata tctcctcatc ttttatacta
     4201 agttgatttt caaaaaaatt cctcttagct atttcctgaa taaattcaga catcattaat
     4261 aaattttctt ttaatacata taatctactt ttaggattta atggaaattc attgtaaatt
     4321 ttgtttatgt attttatatc aataccagaa ctacttgata attcagataa atttttatct
     4381 atatcaatag acttatatac tataaaatta ccacattcta tatatccaac tatattttct
     4441 tctaatacta taggtatctc taaactggaa acaccatact cacaatggta cactttttca
     4501 aagttatcat tatttatgct tttaacaagt tttttagtac acggttttat atttttacaa
     4561 atatcgcaaa atttattttc tttcttagtc tcaaatattc ctttatactt taagtcatat
     4621 acagatattc taagcttcaa tatattagat atttgatttg caagattttc aattatgttt
     4681 acattaacta actcttgtat attgttttca gaaaatacta taggtatttg tctcaaactc
     4741 gaaaatttat gaggaacata agtaattaat attactaaat caattttacc agtgtttata
     4801 atttcatgtt ctgaatatgg tgggcaataa actatagatg tttcctttat attgaccttt
     4861 ttaccatcaa tcatttgtat tccttctccc tgaatcacat ataaaagctg ttcttcactt
     4921 aaatgaaaat gcttttcttg gcagtttccc ggaaaaaatc ttacaattcc agcactaagt
     4981 ctattgaatt gtgtttttga tggttcgtgt atccacataa cctctcccca atcaaatttt
     5041 tgtacttcat tatttgactc aataaaatcc accataaact cacctcgtag tttttatcta
     5101 aatatttttt tattccttta aatatctcct tataatatac attatcataa ttttcttttt
     5161 tattgtatag ataaatagtt atatagaaca ttgtccaatt cttcctaaaa atatagtgta
     5221 aaaaatcttt cctcaaatgt ttccaattga atttactctt tatattagaa aaatcaaaaa
     5281 attatttctt tattctataa ggattatttt ttacacatct ttttttcttt taagtaatat
     5341 attttctcat ctattttatc catacaactt tctagttcat gtattttatt tttaatgttt
     5401 tgcttatgtt gttgaaataa ttggattctt ttatctattg taccatctcc catatcacaa
     5461 aaatgaaagc attcatggat atctttaata gataaaccag tattttttaa ttctttgata
     5521 aatattagac gctctacatc ttcttcagag aaatagcgaa caccacttgg ttttctattg
     5581 ataggaagta ataaaccttt ttcttcataa aatcgaatag cagatgaaga aatttttgtt
     5641 atttttgata catcagttat agaatatttc ataatctcta cctccctaaa ttttatatga
     5701 ataagaatat tataaaaata ccttatacag tagtcaacaa aaaagtattg acttcaagtt
     5761 aggttgaagt tgtactcttt gaagtgtaaa agaaaactta caaaagaaag gatgatataa
     5821 atggaaaaat atgcaactat gattaaagaa aagctaaatg aaggaaaggt cttatgtgga
     5881 gtagctgttt cactgtctga ttcagttgta agtgagttaa tcggtatatc tggatatgat
     5941 tttatttgga ttgaaggaga acatagtgct ttagaccgta aagacattca gcaacatatg
     6001 atagctgccc atgctggtgg tgctgcttct ttggtacgat tagtaaacag tgaccctgac
     6061 cttgttaagc ccataatgga tatgggaaca gatattattt gtttcccatt tattaataca
     6121 gtagaagatg cacaaaaagc cgtagcagct tgtacttatc ctccaaacgg tataagagga
     6181 tataatcctc aaagagcctc ttattatggg aatatgaatt attttgacta tgtcaaaaac
     6241 gcaaacaatg aaacattaaa aatgatgtta attgaacaga aagcaggtta tgataatctt
     6301 gaagaaatag taaaagtcga gggtattgat attatagcat taggccctgg tgacttatct
     6361 ttagacatgg gtttttccgg atatatgaat agaattgaaa taaaagatat gatttctcat
     6421 gctgctgaaa tatgcaaaaa atataagatg ccctttatta tttttcctac aatagaaaag
     6481 aaaagtattg aagaatgggt taatattgga gcaaatatgc tttgttttgc acaagatact
     6541 acctttatca cttatgttat caatcaatta ttatcttttt ataatgagat tgtaccaatt
     6601 caaaaacaac attaaattat aaatctaatt ttatattaaa agagactaat attttatcta
     6661 tctctattaa ggatattttc ttatgtatca taatattagt ctctcttatt attatatact
     6721 gctatcatca ttagttgcta tttatactat ttttataata tacaaattta ttcagctaac
     6781 gttttgccta attctataca ctcatctttt gcttcttcat ttgggtcttc cattgctgta
     6841 attccttctc caccaataat tgtagcacca gcattttgca tacgttcttc ccagtctctc
     6901 atccattcac aatttcccca tccatatgaa ccaaataatc caatctgttt acctgatacc
     6961 atagattcta attctgaaac aaatggctcc atatacccct cttctagttg ctctgctccc
     7021 atagatgggc aacctaatgc aaatttatct tcttctttta aatcacttgg tttcagtaag
     7081 ctcacatcta aaacttctgg tgtcttacct tttaacttca caccttcagc cacaaaattt
     7141 gccatttttt cagtatttcc tgttccactc caataaacaa tatatatttt actcattttt
     7201 acctcctgta gtatttttta attaatcaat agttagttat agctaactaa catctaaaaa
     7261 acaaaagatt tctttatctg aaatctcttt atttttctaa atatagaata tcacaattga
     7321 ttttgaaatt caatatttat tgataatatt tatcatttat attttatatt cattttataa
     7381 tatcaaaaca ctgtatcgta tttctaatct ttcatagaaa aatttctaac ttatagtact
     7441 tttgcatctc ttataaatct tttatatgta taagtacata gaaaaacaca ccttttccag
     7501 atgtgctatc tataatatcg tatatttatt tttcaattct aactagctaa tatgtttata
     7561 aattttatca cttaatacct ttatacattg atgtaaaatc ttacacctaa gtaattttta
     7621 ttatttgtct cgactctata taatatagct attttatcat caaatcaaaa attatctttt
     7681 gctattattc ttgtatttat aaagatttaa atataagtat ccatttcctt gttccgttct
     7741 aggtattcca agcgctctcg tacattttat taattgtgca tacagttcag cttcatctaa
     7801 atctctttca aactcctctc ttgcccattc tttaattagt gctaatgagc ctgatacgta
     7861 tggtgcactc atactagtac cactcataat agcaagttta ttatccatat aagtcgatat
     7921 aatatttctt cctggagcca ccaaatctat tgtagtattt gaattactaa acttttcaac
     7981 caaatagttt tcatttattg cacctacttc tattacctca gcataactgg ctggataact
     8041 atactcactt gtactagaat caccatctcc attattacct gctgcacaca ctacagaaat
     8101 attatttttt actgcttgca tgacagcatt ttttaaattc ttatcatctt tgtttccccc
     8161 aagagacata gatataatat caaccttgtt atttacagca aagttaatag cattaattat
     8221 actttgatat gttccggtac catctttatt taatgctttt gctattaata atttacaatc
     8281 tggagctact cccataactt cattattata gttagatgca gctataatac ccgccacatg
     8341 agttccatgg ccattaaaat cctcatatat atttttattt ccattactgt catcactaaa
     8401 atttgcacca ccaattattt ttccttttaa aagaggatga gatatatcac aacctgtatc
     8461 tattatacca actacaatat ttttaccagt ataaccttca tcccacattc ccctagcatt
     8521 catttgtttt attccatatg ggaatttatt ttttgcacct cttagatttt catttatttc
     8581 atacggaatt agtttcattc ttttattcat catatatcct cctaaaaatt ttattgctta
     8641 taaaaatcat gaattttatt ttataatagg tttataaatc aaattaaaac ctatat
//
LOCUS       DAMKPG010000128         8550 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMKPG010000128 DAMKPG010000000
VERSION     DAMKPG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.133"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(69..353)
                     /locus_tag="PC621_003283"
     CDS             complement(69..353)
                     /locus_tag="PC621_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110413.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     gene            560..1378
                     /locus_tag="PC621_003284"
     CDS             560..1378
                     /locus_tag="PC621_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110414.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1511..3631)
                     /gene="feoB"
                     /locus_tag="PC621_003285"
     CDS             complement(1511..3631)
                     /gene="feoB"
                     /locus_tag="PC621_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF4110415.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3657..3884)
                     /locus_tag="PC621_003286"
     CDS             complement(3657..3884)
                     /locus_tag="PC621_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF4110416.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4211..4651)
                     /locus_tag="PC621_003287"
     CDS             complement(4211..4651)
                     /locus_tag="PC621_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110417.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4803..5792
                     /locus_tag="PC621_003288"
     CDS             4803..5792
                     /locus_tag="PC621_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF4110418.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    5944..6196
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6268..7476
                     /locus_tag="PC621_003289"
     CDS             6268..7476
                     /locus_tag="PC621_003289"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF4110419.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(7537..8418)
                     /locus_tag="PC621_003290"
     CDS             complement(7537..8418)
                     /locus_tag="PC621_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF4110420.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
BASE COUNT         3177 a         1121 c         1220 g         3032 t
ORIGIN      
        1 agtatagaaa tcctattacc tttttattat taattttatc agtgttttat tataaatagc
       61 ttacataatt acacattttt tctgattaaa taatatgatg ctattgttat aatagatact
      121 aatgctaatg atgttattgc agtatctaat cttcttatta ataaactcat ttctatatat
      181 tcaagttcta ttaaatattg ataaaatatt tctagtttaa cagctttttc tctatctata
      241 tttccatact ttaactctaa tttatctaaa gattttttat tacaataatt aactttatgt
      301 ttaattaaat atcttattgt agcacctatt acaattttca ctctaacaag cataaatata
      361 atattccatc caaaagttaa gaggggatat cttttttatg agaggaatgg ataaaatatt
      421 tataactgac atctaactga tatttaagct ttaaaatcaa aaccatattg attttctaaa
      481 ataataacta cttgatttag taaataataa agaatatagt aactatatag acaacaaaaa
      541 gaaaaggagt gtataaccct tggaaaaaca gtattttaca acaggtgaat ttgctaaatt
      601 gtgtggtata tctaagcaga cattaatttt ctatgacaaa ataggtattt tttctcctga
      661 atataaagat aaaaataatt atagatatta ctcagtttat caatatgata cacttgatat
      721 attacaatca ttaagagaaa taggaatgag tttagaggaa ataaaagagt atatacaaaa
      781 tagaactccc cagctttgtg tgaaaatgct taaagaagaa gaaaaaaaga taagagaaaa
      841 aataaaaaaa ctaagaaaga taagctctaa gatgcaaaat agaataaata taacagttga
      901 aggaatttct aaaatgaata gtgaagaaat ttatatatcc aaaaagtttg aagaatattt
      961 agttctatca agcgatttga gtaatatgag taatagtgat tttatgatgg aattaataga
     1021 tttcaccaat tattgtaaat cgaaaaattt gtatcaagga tatgagttgg gagtaatagt
     1081 aagtaatgaa aatataaaaa atggggacta tttaagtata tcaagttttt atttgaagat
     1141 agataaaaaa ataaaggata aaaagctata tgtaaagcca gaaggaatgt atgcttgcat
     1201 aaaacatatt ggaaaatatg aggattcata taaatcttac gaaaaattga aaaaatatat
     1261 atatgaaaat gaatataaaa ttattggaaa ttcgtatgag gagagtatac ttgacttttt
     1321 ttgtgaaagt aatgaagata attatatgac tgaaatttca attcaagtat ccagataaga
     1381 attaaaatag agatatattc aaatgttaag taattttact tatcattgaa tatatctcta
     1441 tgctttataa taaagtatat aaatttttaa atagtttctc taatatatgt ctaatgtcag
     1501 ttaatactaa ttatataaat aaattgtaaa ctgtagttgt taaaaaacaa acaacaaaag
     1561 caagtatagt aggcattaag aatcctaagg cagtccattt taaactacca gtttcttttt
     1621 ttattgttaa aagtgttgtt gcacaaggcc aatgcagtaa actgaacagc ataacattta
     1681 aagctgtcaa ataagtccat ccatgttctc taagaacttg acctaaagca ctaaagctat
     1741 ctaattctat catagaacca gttgctaggt aagccattaa aagtataggt acaactatct
     1801 catttgctgg gaatccaaga ataaaagcaa gtaatataaa accatcaaga ccaattaatt
     1861 tagctaaagg gtctaaaaag tttgcaacat gtgataatat gcttaaatct cctatatata
     1921 tatttgcaaa tatccaagtt atgacaccag caggtatggc taccattaca gcccttccta
     1981 aaacaaatat tgttctatct attatagagg tatataaagt tcttcctatt tgaggaactc
     2041 tataaggagg aagttctaat gtaaatgtag acggaactcc ttttaatagc gtcttagaaa
     2101 gagtataaga aaccaacaat gttattataa cacctagaat tatcagtaaa gttatgcaaa
     2161 gtgcagttgc tacgcttgat acgaaagagt tggttataac tgagctaaaa aatattgtag
     2221 atatagctat caaagtagga aaacgaccat tacaaggaac aaagttgttt gtaagtatag
     2281 ctataagtct ttcccttgga gagtcaatta tcctacaacc aataactcca gctgcattac
     2341 atccaaatcc catacacata gtcaaacact gttttccatg tgcacaagct tttttaaata
     2401 ggtggtctaa gttaaatgca actcttggca aatatccaaa atcctcaagt agggtaaata
     2461 atggaaaaaa tattgccata ggaggtagca ttacagagat tacccatgct aatgttctgt
     2521 ataatcctaa aaccaacata tcatttaacc atgatggaca attaatacta tttaatatac
     2581 ccgaaatact tggctcaaat ccgagtaata aattagataa taaagtggat ggatagttag
     2641 ccccttctat agtaatccac aatatagtac ctaataataa gagcattatt ggaagcccaa
     2701 atattttaga tgttaatatt ttatctactt tttcttctct atcagttgat tttttagcaa
     2761 cattagaaca acactcatca ctcagttttt tagcataatc atagttgatt ttagtaaact
     2821 catctcttat tttctgttta tttatattgt ctggaatctt tttctttact tcattaatag
     2881 catctataga atctttgtct atataattac tcatagattc aaatatactt tcatcgccat
     2941 caataagtct aagtcccaac catctagaat ttatacctgg tataatatta tccaattctg
     3001 gttgaattga ttttaccaca ttctctatat tttcattgta tctaacaggt ttattattta
     3061 atttgtattt gtcaaaagat acatcattta aagtatccaa tagttcatcc ataccagagc
     3121 catttcttgc agcagttaaa ataactggaa taccaagaga atcctctaaa agttttttat
     3181 caatagtaat gccttttttt ctagcttcat ctattaaatt aatacacaaa ataactttat
     3241 ctgtaagttc catcacttga aatactaaat ttaaatttct ttcaagacat gtagcatcac
     3301 atactactat tactgcatct ggattaccaa aacatataaa atcacgagct acaatttctt
     3361 cttgtgataa ggcaaataaa gaatatgttc ctggtaaatc tattaatgca tattcggtat
     3421 ttttatattt aaaattacca cgagctgtag ctacagtttt tcctggccag tttccagtat
     3481 gctgtctaag ccctgtaaga tgattaaata cagtactttt acctgtattt ggattgccag
     3541 ccaatgcaat tacaaactgg tcttccttat tgtctatatc aaacatatct tttaaagaac
     3601 tcattttagt tgagttatgt gtcaacccca tattaatatc ctcctgctag aaatttttaa
     3661 atgtcagaaa ctaatattaa gctactttct tcttgtcgta gagctatctt actacctctg
     3721 attttataga cagttaaatt attttttgga ccttttctaa ctacatctat aacagctcct
     3781 ctagtaagac caagcgctaa cattctttct cttaaattac cactagataa tatatcttca
     3841 actttgacag tttttttcac ttgaatatcg ttaaggtttt tcatagtagc acctccatgc
     3901 ttgaaattta gcctgggcta atcttcttat tctaaactat gcattatatt attaccttgt
     3961 tacttttaca aaaaaaagat aggcaagatg cctatcatac aaagttgggt ataattctta
     4021 cttttgggga aagtagaata tagaataaaa attaacactt tgaatccata taattattga
     4081 attttgataa taaaaaagtc agaaagcaaa agcctcctga ctctatgtaa ggtattttca
     4141 aatacctaaa tatcaattca atatttaatt tatacttaaa ttatatatta acaatattag
     4201 cttgtcaaca ctataatctc attttaatat gttcaatacc tgcttcgtcg tatacttctg
     4261 agatttcttt aaaaccacaa gataagtaaa ggttctttat ataagtttgt gctgataagg
     4321 ttatattatt ttcgtgtaag ttaattttta taaaatctat agcacaatcc atcatttgtt
     4381 ttgctaatcc ttttcttcta tgactagaaa gaactaaaac tctaccaatg gaaacatcat
     4441 tataagagct gtattcttta ggaattattc tagcataagc acaaatcaat ccattttctt
     4501 ttaaaaatat gtgataagag cttttatcta tatcatcata atcgtttaat gaaaatatct
     4561 tctgttcaca agcaaatacc tcatatcttg attttgctat ttcataaaat tcatctaaac
     4621 tcaaatcatt aaagtgttta attttaaaca tagtaattac ctcctagcat cttaaaaaac
     4681 tgaatattaa gatacattat accattttta acataaatac ctttattaaa atttatattt
     4741 attaaaaata tttttaaaaa gtgatatact atattaaaaa caaaacttta gttagaaaat
     4801 ttatgattta tggaggcagt gttttgaata ctactaaaaa attaactgaa gcagctttat
     4861 tgtcatcttt attcatagta gtaacaatta tagctgtgag tactggattt ggatatgcta
     4921 tttacttaga ttttatagtg ccagtatttt tttgtataat ctgcttgaaa tgtgatttaa
     4981 agtataccat tttatctgga attacttcac ttttaataat ttctctggta cttggaaatc
     5041 taggaactgc tatttgggca agtcagagtg tattacttgg tataatgtgt ggctatctaa
     5101 taaataagcc tacaatggtt atggatgatt tagtatatgg ttcagtattt ggggttattg
     5161 taatggtttt cattgatata tatgcttcaa cattaatagg ttatagtttt atgaaagaat
     5221 ttcaaggata tagcaaatgg atttatttta atgggtatac aaattttatt tattatttaa
     5281 tgatagcatt gtttcctttt ggtatggtgt tttgcatata tttattaagt ttaattttag
     5341 ggaataaatt gcatatttta gattctaatt ctttaaaaaa atttttaata tttaagaatt
     5401 ttagaagttt aaaccaattt ttatgttgct caaaaaaagc tttttacata tgtgtttctt
     5461 atctagccat ttttgaagtt ctaaaactgt tgaatgttaa agttgattct gtttatttaa
     5521 aaacaatctt tatatcaata acctatataa gtgtctattt tatcattaga gactcatgta
     5581 tgtcactaca aaactttata atagctaagt ttagaaaatt attatatgct agaattttat
     5641 ttttgataat aattttattg ctgtttttta tatttgatgt aacagttatt agtttaataa
     5701 ttataaacgc atttttaaat aagaaaataa atataagatt gagtcagagt aatatagtca
     5761 ataaacaaat aaatcttttg attgaaaaat aaactttggg tattggtaaa aaatataaaa
     5821 aagtagtata atgtaaaaaa acatgtatat aataataaaa aaggttgaca aaatataaaa
     5881 ataataatat attatgagta agttaagaaa aatttcttgt aattgatgaa ataatctgaa
     5941 tatataaatg ctatgaagag aagaagtagg gctagatttt atttaacaga gagttccagt
     6001 ttggtgaaat ggaataaatt gatttagttt gaatacatct cagagcacta tctctgaaag
     6061 ataacaacca ttaagagata tcggattagt acacgttata gtacacgagt atatgtaatt
     6121 gtactctaag agattaatat cgtgagatat tgatgaacta aggtggtaac acgtaagcaa
     6181 tgctttcgtc cttttaagag gatgaaagct ttttttatta taaaaaaaaa attatagatt
     6241 atataaatat tttaggagga atgaaaaatg aaatcgatag acgagcaaat gcgaataatt
     6301 atgaaaggtg tagatgattt aatagatgag aaagagttaa gggaaaagct tattaaatca
     6361 gaaaaagaag gaaagcctat gatagtaaaa ttgggtctag acccaagtgc tccagatata
     6421 catttagggc atacagtagt tcttagaaag atgaaacaat tacaagattt aggtcatcaa
     6481 atagtaataa taattggaga ttttacagga aaaattggag acccaactgg aaaatctaaa
     6541 gctagaaaag ctttaacaac agagcaagtt cttgcaaatg caaaaactta tgaagaacaa
     6601 attttcaaag ttttagataa agaaaaaact attgtaagat ttaatagcga gtggttagcc
     6661 aagttaaatt ttgaagatgt aataaaatta gcagcaacta ttactgttgc gagaatgtta
     6721 gaaagagaag attttaaaaa gagatatgaa ggacaaatgc caatatcagt acatgagttc
     6781 ttctacccat taatgcaagc atatgactct atagcattag aagctgatat agaacttggt
     6841 ggaactgacc aaagattcaa tttactaatg ggaagatcat tacaaagaga atttggtatg
     6901 gagtctcaaa tagtaataat gatgccttta atagaaggat tagatggtaa agaaaaaatg
     6961 agtaagagtt taggtaacta tattggaata gatgaagaag cgggaataat gtaccaaaaa
     7021 tctatggaaa ttcctgatga acttataatt aaatactaca atttagtgac agatgttcat
     7081 cctgatgaag ttaataaaat agaatctcag ttgaaagagg gttcagtaaa tccaagagat
     7141 ataaagatga acttagcaag agaaatagta acattatacc atggagaaga atctgctaaa
     7201 gaagctgaag aaagatttaa atctgtattc caaaaaggtc aaatacctga agatatacaa
     7261 acaattcaag ttaaggaaga tggatttgac ttaatcgaag tactagtttc taatgagatt
     7321 gttaagagta agagtgaagt tagaagatta gcttctcaag gtggagtaaa ggtaaatggt
     7381 gaaaaagtag aggatttaag cactattgta aaagagagtg aattagttgt tcaaatagga
     7441 aagaaaaaat ttgtaaaaat cgagctagta aaatagagta aagtaaaagc aagtctattg
     7501 agtataaaat gtacattcaa taaacttgct tttttattat attaaaattt taaattgata
     7561 accattgtct ttaaaatatg ttataatttg gtccaatgca tttactgttt cttgttttcc
     7621 ataagtatca tgcattaaaa gaacaaccat ttctttgcct tcagaagatt tctttgcatg
     7681 ttcaaataat tcttgtgcat ttttcttttt accttctgca tcagcattta aagcgttcca
     7741 atcaattgat gccatgtttt tctcttttaa gtagtttcct agaggttcca tatttttcca
     7801 tgacatatac ccgcctgggc atctaactac atttgaggaa aaattctttc ctaagacttt
     7861 tttcatagct tcatcagttt tgttaagttc atttacgaat gtatcgagat ttaggcttct
     7921 gccaggatat agttttttat agtcatgggt ataagagtga tgggctattg catttccttc
     7981 gtcaaatgtt ctttttaaaa tttcattagc tccttttctt tcaagagaat cacccttaat
     8041 aaagaaagtt cctctaacac catgtctttt caagatatct agaacttgtg gagtattagt
     8101 agtagatggt ccatcatcaa atgtaagaaa gactatcttt tctccattgt ttgaatagtc
     8161 gtatttgtta agtttgtccc aaactatttt tgcgtcatat ccatattctt ttacagcttc
     8221 tttttttgca ccaataatag gttcattttt ttctttttca attctttctt tttctaatct
     8281 ttcagcttta agtctttctt gagtttcttt catttctttt tttctagaca tgtccgccca
     8341 tgccatagaa aaaataccaa taactattac aacaagtata gtaagtgtaa cagttttctt
     8401 ttttcttttt tttaccataa ttacagcatt cccccttttg tcgaattgta tcaaaaatat
     8461 attataatta tatcgaagaa agtagaaaaa gtaaaccgac aaagttgtaa ctaagataac
     8521 taaagtgtaa cttatatgac attatttgca
//
LOCUS       DAMKPG010000129         8491 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMKPG010000129 DAMKPG010000000
VERSION     DAMKPG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.134"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            16..2193
                     /locus_tag="PC621_003291"
     CDS             16..2193
                     /locus_tag="PC621_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF4110421.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2207..2629
                     /locus_tag="PC621_003292"
     CDS             2207..2629
                     /locus_tag="PC621_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4110422.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3036..3839
                     /locus_tag="PC621_003293"
     CDS             3036..3839
                     /locus_tag="PC621_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF4110423.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            3832..4500
                     /locus_tag="PC621_003294"
     CDS             3832..4500
                     /locus_tag="PC621_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF4110424.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            4736..5398
                     /locus_tag="PC621_003295"
     CDS             4736..5398
                     /locus_tag="PC621_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF4110425.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5458..6255
                     /locus_tag="PC621_003296"
     CDS             5458..6255
                     /locus_tag="PC621_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110426.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            6388..7563
                     /locus_tag="PC621_003297"
     CDS             6388..7563
                     /locus_tag="PC621_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4110427.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            7672..8421
                     /locus_tag="PC621_003298"
     CDS             7672..8421
                     /locus_tag="PC621_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF4110428.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         3366 a          863 c         1582 g         2680 t
ORIGIN      
        1 atttgaataa aaagaatgta taataagtat aataatttgt atttattatt gggaaaggaa
       61 aatgttaata tggatagtta tagacaactt aaagctaaaa taccctgtaa ggaaacaggt
      121 atagaaataa agaggacttt atgtgatatt tgttctccag gagtacattg tggaattgat
      181 gcatatgtta aagatggtaa agtaataaaa atagaaggta caaaagaaca tccaatgaat
      241 aaagggttgt tatgtaccaa aggtctttgc aatagagaat atatatatag agaagataga
      301 ataaaaacac ctctaaaaag aattggaaaa agaggtgaag gaaaatttaa accaatatca
      361 tgggatgaag cttatagaga aatagcagaa aaattaaata gagtaaaatc tgagtatgga
      421 gcaaagcatg tagctttttt tacgggttat ggaaagtggt atagaccttt tttgcaaagg
      481 tttgcatctt catttggttc acctaattat ggtacagaat ctagttcatg ttcaacatca
      541 gcacagatgg ctggaaatgt tgctactgga agaaacttta caatgcaaga tatggagaat
      601 gcaaaaactt atttagggtg gggatttaat ccatactact ctaaattttt aaatagtgtg
      661 aatctaataa aattaaaaga aaaaggtcta aaatttataa ttgtagaccc tagaataaca
      721 ccgacaactc aaaaactagc agatttacat ttaagaatta aacctggaac tgatggagct
      781 ttagctcttg gaatggcgaa tctatttata caaaaggatt taatagatag agaatacata
      841 gaaaagcatg tacatggatt taatgaatat aaagaatatg taaaattatt tgatttagat
      901 actgtaagta gaattacagg agtttctaaa gataatatag taaaagctgt tgagatgttg
      961 tcacaaggtc cattttgtat aaatgaaagt gcagctccaa tagttcatca tcaaaatgga
     1021 tttcaaaact atagagcaat gatgtcttta tctgctataa caggtaatta tgatagaata
     1081 ggtggaaaca taccaaaaca agattacttt gctggtaaga taggtggatt ccaaacacta
     1141 gaaaaagagt ttgtaaaaga agttctacct aaaaatataa atgaaagaat tggtggagaa
     1201 agatttcctc tatggaatga aatggtaact caatttcaag ctatggattt acctaggcaa
     1261 atagaagaaa agacaccata tgaaataaga gctttgtttt gtcatgggat aaatgctaga
     1321 atgtttccta attcagggag actttttaaa gcgttgcata gtgtagattt ttttgtgaat
     1381 atagatttat ttatgacaga tacatcaaaa tatgcagata tacttttacc tgcgtgtaca
     1441 tcatttgaga gagaagaatg taagagttac ccaggagggt atattacata taccaaaaaa
     1501 gttattgata aattatacga ttcaaaatca gatgtagaaa tactttcaga tttagcaaat
     1561 gttatgaata tagatgatga tttattaaaa gcaggttatg aagcaagtat aagacatatg
     1621 tttaaaaaga cgtgtgtaga tgtagataaa cttaaagaaa gtgatttacc attaaaatgt
     1681 aaagatttta agccatatat agtaggctca tatacaagag aggggtatga tactagcact
     1741 tctaggtttg aattaaaatc tacaattatt gaaaaatata aagattttgg actggatgca
     1801 ctaccaacat atagaggatt taatgataat atagataatg aatatactca tttattgaca
     1861 tcaggagcaa gaatttcaaa tgcactacat tcaagacttc atgacatcaa actttcaaga
     1921 atattaaaaa atgaagctag ttgtgatatt tccagcttgg atgcaaaaga attgggtgtt
     1981 atcagtggag attatataaa aataactact aagataggaa gtttggatgt aaaagtcaat
     2041 gttactgata gagtcttata taagaatata catatgtatc atggatatag agaatctgat
     2101 ataaatagta ttattgatga taattttgac ccttatagtg gattccctgc gtatagaagt
     2161 tgcaagtgta agattgaaaa aatgaataga taaatggaga gatataatga aaagtttatt
     2221 taaatttaat aaagaattat gtacagcatg tggagcatgt ttgcttgcat gtatagacca
     2281 aaatgataat aatatagaca atggtgaact ttttagacat gtatttacaa tagaggaagg
     2341 tgaaaatata gaatattttt cattagcatg tatccattgt aatgagccaa aatgtttagg
     2401 tgcatgtcct aaaaattgtt ttaaaaaaga agaaggattc gttgtattag acaatcaaaa
     2461 ctgtataggg tgtaaacttt gtgaaaaagc ttgtgaatat ggagccttaa tttatggaaa
     2521 tgatggtaag gcaaataagt gtaatggttg tattgagaga tataagtgtg gtatggattc
     2581 accttgtgta atggtatgtc ctacaggagc attaaaattt aataaataaa ttaaatatag
     2641 agagtgactt atacttggta taagatattt ttacaaaaag ctatattgag tttgttataa
     2701 taaaaactca atatagcttt tttatggata tagttggata ctataatgtg atttttagta
     2761 atagtttttt gctattggac aaagactgta aaacatatta aacttatgtt gttcaaagtc
     2821 gaagtataat ataataacta gaaaattgat ttatagtgtt ataaaagcaa atttagtaag
     2881 gtataaaata agaggcaatc ttttactaca gtatcaatat ttgggagtta tgttatggca
     2941 gagccattaa acaattataa tggtacaagt gattcaacct ttgaaaatgt attatataaa
     3001 aaattaaaat aagattaaaa aggagagaat aaattatgaa aaaacttaaa ttaattatta
     3061 tgcttgcctt ggtattagca cttttaatta caggatgttc atcaggaaat aataaaaata
     3121 aagaagaaca aaaaactata agagttgcaa catcaggaac atattaccct tttgctttta
     3181 aagataaaga taaacttaaa ggatttgaag tagatttttg ggatgaattt gctaaacgaa
     3241 ctaattgtaa agttgaatgg gtattaactg attttagtgg attatttgga ttattagaag
     3301 ctgataaagc agatgttgta tctgctcaac ttactgcaac acctgaaaga gagaaaaaat
     3361 atgctttttc agatgcttat aattatagtg gaacaaacat tattgttaga gaagatgata
     3421 ctagtataaa atcaatagat gatttagttg gcaagaaagt aggtgtgggt actggagcag
     3481 tagcaaatga aatactaaaa gaaaagtacc caaatgatga aataaaaata gtaaattatt
     3541 caagtgcaac tcttaaaggg aattatcaag atctagaaat gggaagatta gatgcagtag
     3601 ttgcgcagga tgttgaggct ttaatagcta taaaagaaca aaaattaaat cttaaaatgg
     3661 tagaacctcc aattcaattc ggagcctgtt cttttgcgat tcaaaaaaat gaaaaaggtg
     3721 aaaaatggac aaaagaaatt aacgaagtaa ttgaagagat gcataaagat gggactttga
     3781 ctaaaatatc tgaagcctgg ctaggaaaag acattactaa ggagcctatt aatgagtaat
     3841 tttaattttg aattttgtct aagtatattt ccatttttga ttaaagcatt gggaataaca
     3901 atactaatat ctattgttgc atttttttta tcaatattat taggtgtaat acttgccttt
     3961 ttcacacaga caaaatcaaa agttttgcaa tacataacaa gagtgtatgt ttcattcttt
     4021 agaggaacac cacttttaat tcaaatattt ttcttttact ttggatttcc aatgatattt
     4081 tcttttatga aagattgttc accttattta gcaatagtaa tttgtttagg tgtaaattca
     4141 tctgcatata tgactgaaat tatacgagca tctattgaga gtgttgataa aggacaacag
     4201 gaagcatgtt atgcttttgg gttaagtaaa tatcagtcaa tggttaaaat aattctacct
     4261 caagcagcta catctgcagt acctcctatt gtaagctgta cactggacat aattaaaggt
     4321 tcttctttag gtctaactat aggaatacaa gatttaatgg gagtagctca agtagaggct
     4381 gcttcaagtt ttaaatattt tgagatatat atgataacga tattaattta ctgggcagta
     4441 tctataatat tgagtaatat acaaagcaaa gttgaaaaaa gaataggaaa ggcatattag
     4501 atttcaatat ttaaaaatgg gatgatgtag atatattatt acttgtaaat aaatttttaa
     4561 ttttagaaaa ataaacttag taatctaaat tattgagaaa atatagtatc aaaatggact
     4621 gtataatcaa gttaaaaaat agtttttcta ttgaaaaaat aaataaaatt ctattgtaat
     4681 ttaatattcg acaaagtttt ataaaaaaca acattaagct agggggactt attttatgat
     4741 ttttaatatg aaatattttt tagaattatt tccaataata atgaaatcgt tcaatgtaac
     4801 tgtaattttg gcaataagtt cattagtatt ttcattaatt ataggtacaa tagtagcatt
     4861 atcatcttat tacaaagtga aagtattgaa tcaaatatgt aaagtatatg tatctatctt
     4921 tagaggtacg cctcttatgc cacagttatt ctttttatat tttggactag cgtatatgaa
     4981 cgattttgtt aaaaatatgg acccagtatt tgcaacatct atagtactta gtttaaatat
     5041 gggagcttat atgtccgaaa cgataagggc atctatactt tcaattgata aaggtcagat
     5101 agaagcagca tatgtaatgg gattaacaaa tttacaaaca atgaaaagga tagtgatacc
     5161 acaggccgta agagttgcac tgccatcatt attcaataat tttatagatt taattaaagg
     5221 gtcttctgta gcttttgttg ttggagtaaa tgatataatg ggtgctgcca aatcacaagg
     5281 agcgttgagt tttaagttct ttgaagtgta tggagcagtt atggtaattt actggtgcat
     5341 aataactcct ttagggtggg ggcaaaaaaa attagaaaag aaaataagca attattaagt
     5401 tattaagaaa atacataaaa gtatttttat ataaaaaatg gtgtgaggag aagagatatg
     5461 gtaaaaagaa atattataaa atgtttagtg gcagttttat cgttaacatt tgtagttgct
     5521 ggttgttcaa aaaaagagga agtaaaggat gaaaaagtta taactgtagc ttcatctaat
     5581 tcagctgacc catattctta tgttgataat ggtgagcata agggttttga agttgatatg
     5641 tggaaagaga ttggtaaaag aagtggatat aagataaaaa tggaaccaac aggttttagt
     5701 tctatatttg ggatgttaga ttctggtaaa gttgatgtgg cagccaattt ctttggaatg
     5761 tctaaagaaa gattagaaaa atacgatgct tctatacctt atggttctga ttctgtatgt
     5821 atagctgtaa aagatggaaa caacgaaatt aataaacttg aagatttaaa aggaaaagtt
     5881 gtagcagttt cagaaggttc tcaagggcaa caggttgcag ctagtgttaa taaagataat
     5941 ttgtttgaag aaaaggttta tggtgatgga actaatggtc ttcaagattt agatttagga
     6001 agagttagcg catggattga tgcagaactt actgttgtag gagacgctaa aaaagctaat
     6061 atgaaggtaa gagtattaga tgaaaaatta tcagttacta atatagcata cttctttaag
     6121 aaaaatgatg aaaaatctaa aactataaaa gaagatgtaa ataaagcaat agaagaaatg
     6181 ctttcagatg gaacagtgaa aaaaatatct gaaaaatggt ttggaatgga tgtaagtgct
     6241 gatatacaaa aataaatact ttaaaattaa aatataaaac tgtgataact atagataagt
     6301 atactaaatt tatctatagt tatctgtgta ttacaatgta tttagattag tctttataaa
     6361 ttaaaatgag ttatggaggt cttgcaaatg attgaaacta aaataaaatc cattgcacta
     6421 aaaaatagag aaaaaatcat aaaaataaga cgacagatac acagtaatcc agagttagca
     6481 tttaaagaat ttaaaacatc caaacttata aaagaagagt taaataaact taaaatagaa
     6541 tacatagatg ttgctggtac aggagtatta gctactataa aaggaaaaaa taatggtggg
     6601 aaaactatat tgcttagagc ggatatggat gcccttccaa tcaaagagga aaatgattta
     6661 gaatttaaat caataaatga taatatgcat gcttgtgggc atgatgccca tgtttcatgg
     6721 ttgttaggaa cagcaatgat tttaaatgac ataaaagaag aattgaatgg aaatgtaaaa
     6781 ttactatttc aacctggcga agaaaaaggt ggttctgata ttgtaattaa agaaaatgta
     6841 cttgaaggtg tggatgcatt agcaactggt cattgttggc caactattga atcaggaaaa
     6901 attggaatcg ctagaaattg tgctatggca gcgactaata cttttgaaat aacaataatt
     6961 ggcaagggcg ggcatggagc agagcctcat aattgtatag acccaattgc agttggaaat
     7021 gcagtatatt catccattca acagatagtt tcaagaaaaa ttgacccagt tgtacctgta
     7081 gtagtgtctg tttgttcttt taattctggt gtttctaaaa atataatacc agatgtatgc
     7141 acacttcaag ggactattag agcgataagc aaggaacaag taattgaagt aagtaaaatt
     7201 ctagaaaata tagtaagaaa tgtatgtaga gcatacggag cagattgcaa atttgaaaag
     7261 agtacaggag gagatgctgt tataaatgac aaaaacatga tagagctagg aagagaaagt
     7321 gcttgcaaaa tacttggagt tgaaaatgtt gaaataattg attttcctgc aatgacaggt
     7381 gaggattttg ctatttatat gaaagaaaaa cctggtttat ttatgtatat aggtgtagga
     7441 aataaagaaa aaaatataaa ctacagattg catagtaaca agtttaatat agatgaaaaa
     7501 tgtcttagta tagcatcttc attattctca caacttgcag tagattattt atgtcaacta
     7561 tagagtacat tcataaaaat atatttagcc tatttttaaa tttaatgcta gtatattaat
     7621 tttgatgtag aacatattat ttattatatt tatttaaaaa ggtggataac aatgataaaa
     7681 gtagaaaatt tacataaaag ttttaaaggg acagaagttc ttaaaggtat aaatttagag
     7741 ataaatcaaa gtgaagtagt agctatactt ggaccatctg gaacaggtaa atctacgtta
     7801 cttagatgtt taaattattt agttgagcct acaaaaggaa agataatgat tggtgatgta
     7861 aaagtagatg tagaaaaagc aacaaaaaaa gatatagcca atttaagaaa acatacatct
     7921 atggtatttc aaaattataa cttatttaaa aataaaacag cattagaaaa tgtaatggag
     7981 gcattgatag tagtacataa aaagagtaat aaagaagcta gagaaatatc tttaaaatta
     8041 atagataaag taggtatgtt ggataggaaa gatttttatc catcaaaatt gtctgggggg
     8101 cagcaacaaa ggataggtat agcaagagcg atggcagtaa atccaaatgt aatattattt
     8161 gatgagccaa catctgcact tgacccagaa cttgttggag aagttttaag cgttataaaa
     8221 tcgcttgcga aagaaggaat aacaatgctt attgtaactc acgaaataaa ttttgcaaaa
     8281 gaagttgctg ataaaattat atttatggat aatggtgtaa tagctgaaga aggaactcct
     8341 gatgaaatat ttaataatcc taaaaatgaa agaactgcaa aatttttaaa tatcgttagg
     8401 aaagaagaag tatttagtta aaagtgtata aattaagtat tagaagatat gtaagatact
     8461 cccattaaat ttataagtta gataataaat t
//
LOCUS       DAMKPG010000130         8461 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMKPG010000130 DAMKPG010000000
VERSION     DAMKPG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.135"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            72..2183
                     /locus_tag="PC621_003299"
     CDS             72..2183
                     /locus_tag="PC621_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF4110429.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2381..3346)
                     /locus_tag="PC621_003300"
     CDS             complement(2381..3346)
                     /locus_tag="PC621_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF4110430.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3631..4587)
                     /locus_tag="PC621_003301"
     CDS             complement(3631..4587)
                     /locus_tag="PC621_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF4110431.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4955..5743
                     /locus_tag="PC621_003302"
     CDS             4955..5743
                     /locus_tag="PC621_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF4110432.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5866..6336)
                     /locus_tag="PC621_003303"
     CDS             complement(5866..6336)
                     /locus_tag="PC621_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF4110433.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6475..7119
                     /gene="uppS"
                     /locus_tag="PC621_003304"
     CDS             6475..7119
                     /gene="uppS"
                     /locus_tag="PC621_003304"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF4110434.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            7162..8067
                     /locus_tag="PC621_003305"
     CDS             7162..8067
                     /locus_tag="PC621_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4110435.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT         3300 a         1013 c         1342 g         2806 t
ORIGIN      
        1 atgaatttta gatatactta ataagaagta tattgtagaa aaaataaatt taaaaaatta
       61 agggggatga aatgaaaaaa ataagtatat tagtattatc attgataatg acacttacta
      121 tgtgtagtgt aagttcattt gccgattcat caaatgataa ggaaatgaga gctgcttgga
      181 tatctacagt ttataattta gactggccta agactaagaa taatgaagcc aagcaaaaaa
      241 aggaatacac agatttattg gacaaactaa aaagtgtagg aataaataca gcagtggttc
      301 aagtaagacc aaaatcagat gctttatata aatcaaatat aaacccatgg tctgaatatc
      361 ttaccggtac acaaggtaaa gaccctggat atgacccatt accattttta atagaagaag
      421 cacataaaag aggaatggaa tttcatgcat ggtttaatcc atatagaata actatggcag
      481 atgagagcat agataaattg ccagcaaacc atccagcaaa gaaaaatcca agctgggtag
      541 ttaaacatgg aaataagtat tactacgacc caggtttacc agaagttaga aagtacatag
      601 tggatagtat agcagaagta gttcaaaatt acgatataga tggagttcat tttgatgatt
      661 acttctatcc aggagtaagt ttcaatgata ctgcaacata ccaaaaatat ggaaaaggac
      721 aaaataagga taattggaga agagaaaatg taaatactct acttagagat gtaaaagcgt
      781 ctataaagag tataaagcct aatgttgtat ttggtgtaag cccagcaggt atatggagaa
      841 ataagagtag tgacccaaca ggttcagata catcaggtaa tgaaagttat gtaggtacat
      901 atgcagatac aagagcatgg ataaaacaag gattgattga ctatgtagtt ccacaacttt
      961 actggccaat tggtcttaaa gcagcagatt attctaaatt agtggcatgg tgggctaatg
     1021 aagtaaaagg aacaaatgta gacttataca taggacaagg tatatataag caaggtcaat
     1081 ctagttatgg tggacaaaat atagcaaaag aaatagtaca acaagtaaca ttaaatagaa
     1141 agtatagtga aataaaaggt agtatgtatt tctcagctaa agatatagca aatagtactt
     1201 ctatacaaaa ggatttaaag agtttatatt ctagtagtga agaaccagtt actccaccat
     1261 ctaatgttaa agtggagaaa ttaagaggcg acgaaagata tgacacagca gtagcaataa
     1321 gtaaaaaagg ttgggctaca aattcagata cagtagtatt agtaaatggg tactctatag
     1381 ttgatggcat aacatcaaca ccacttgcta catcaaatga tgctcctata ctattggtaa
     1441 ataaggataa tatacctaca tccactaaga atgagttaaa aagacttaat ccaagtaaag
     1501 ttattttaat tggaggaaat aactctattg gagataaagt tgagagtgag ataaaagata
     1561 cattgtcaaa tgtatcaata aatagagttg gtggttcaga tagatatagt acatctttaa
     1621 tgatagcaaa agaattagta aaaactaatc cagtagaaaa actatatata acaagtggta
     1681 caggtgaagc agattctctt tctatagctt caaaagcagg agaagaaaag caaccaatag
     1741 tactagtatc aaaagataat gtaagtgatg aagtttacaa ttggatatca gatttaaaag
     1801 taaaagatgc ttattttata ggtggtaatc taagtatatc tgattcagta ataaataagc
     1861 tggataaggt cattacaaat gatgtatcta aaaatagaat agctggagaa aatagagaag
     1921 aaacaaatgg aaaagtaata caaaaattct atccaaatgc tgaatattca tcaatgtttg
     1981 taagtaaaag taatcaattg gtagatgcat taacatcagg tcctttagca gctaaattaa
     2041 aatcaccagt agttatgctt ggaaatagtg taacatctgc tcagaaaact gctttagagc
     2101 ctaagaaaac tgctctagta tacgaagctg gagatggtat aaatcaaaac acattaaaca
     2161 ctttcttaaa cttagttaag taataaaaaa gttaatttaa aatacagagt attttataaa
     2221 tgtagattaa aatgaaaatt atcatcataa attagaaaat aaaaaagaac tatatgcatt
     2281 ttgtaatcaa gtgtacacag caacttgaaa ataattatgc gatatagttc ttttttagta
     2341 attaatctta tactttttaa taaactttat gttgaatttt ttattgttta aactctatgt
     2401 cattaaatat aaaatcgtgt aatatgtcta aagtatctgg gtcaaatcta agaacccatc
     2461 cagcttttcc atatatacca cctgtactat gaatttcatc atcaatagga aattcaaatt
     2521 gcttaatatt taagtcaccc atttttaaaa cttgagctcc taaagaaagt attgcattag
     2581 gcttcatgtt agttttaaca tatggaagaa ctgcatctaa taaacctgga tatttagtaa
     2641 caggtaaagt ttccgttttc ttgattatag cttgtataat ctgtctctgt ctgccatctc
     2701 tagcaaaggc tgtatcgttg tgtctgatac gtgcaaaaga tagtgcctga tatccattta
     2761 gagtctgctt tccagagttt ctaatgtatt gtatgctacc tttatcatca cttttattcc
     2821 atttataagt ttcaggaata aatttattta gttctcgtat ttctcctttt tcaacagtaa
     2881 cctcaacgcc accaatagca tcaattatat acataaatga ttcgaaatta acacaagcat
     2941 aattttgaat atcaatatta aaattctctt caattgtttg tattaataaa tcagcttgtc
     3001 cataagcata agcatgagta agtttttgtt taccatgacc agggatgtct acatagctat
     3061 cacgagctaa tgatgtaagt ttgatatcat tgtgtttgtt atcaatagta agtatcatca
     3121 tagcatctga ccttgaagaa tcttcatttg gtctagcatc tgttcccata agtagtatgt
     3181 taattatccc atcttcattt ttatggtcat tgttatccaa taaatcacta cttatgctcg
     3241 agtcatgaat agcagataat ttataataaa aatatccata gacacttata gggaatatta
     3301 ctaccaagaa agccagtagt ataacaaatt tctttaattt tgacaaaagt accccttctt
     3361 tcttcttttt cctgtatatt ttttaatagc atacacttaa ttatattaaa gtattgagat
     3421 atattcaata cttgtttcag ttacaaaaaa aagacaatga tataatttat aagaaaattt
     3481 atcgacctat atgtatagtt aaaagtatat ataatatatt ttttaaaagt aatttatatt
     3541 ttaaaataaa aacacacccc ataaatacaa agcaatttgc atttaagagg tatgtattta
     3601 tcatcaaatt attggtttgt tttggaattt ctaatcttca accataatat ctttaaatat
     3661 gaaatcatgc attgcatcta gttcatattc ctcaaaaggt attacatagc ctgcattacc
     3721 tatttttcta ccattttcag tagaaagagg aaactccata gatttcaaat ttaaatttcc
     3781 tatacttaaa agttcttttc ctaaagatat tatttcatta ggattcatat tagtttttac
     3841 atacggaagt attgtgttaa ctagattagg atatttagtg actggtaaat ccttaacacc
     3901 atttattaaa ctctgtataa cacttctttg tcttctatct ctttcatctg tactatcatt
     3961 ttttcttata cgagaaaatg caagtgtctg atatccattt aatttttgct caccagaatt
     4021 tcttatatat ttaagagaac ctttattgtc atttttattc catttataag tttcaggaat
     4081 aaatttattt aattctctta tttcactctt atcaacagta acagtaactc cacctaaagc
     4141 atcaactata tccataaaag agaaaaagtc aactacggcg tagttttgta tatcaagttg
     4201 gaagttttct tctatagttt ctattaataa gttagcttgt ccatatacgt aggcatgtgt
     4261 taatttttgt tctccacgtc ctggtatatc aacataagta tctctattta atgaagttag
     4321 cttaagactt ttatttttat tatcaatagt aagtatcatc atggcatcag aacgagaaac
     4381 ttcttctcct ggtcttgcat ctgtacctaa caataatatg tttgtgatat tttcttcgtt
     4441 tttatagtca ttactactaa gcgcctctgt atttatacta tcatcttgta tagtttttaa
     4501 tttagtaaaa acatatccat atatactaac aggaaatgct ataactaaaa gggctagaaa
     4561 tataacgaac tttttgagtc ctgacaataa tatcccctac tttctaaatt ttttaattac
     4621 acatagttaa ttatattaaa aatttgttac ataagcaata aaaaacacaa taaattagca
     4681 taagtttgtc ataatataat tatttttata gtattttata gaagaaagtg tgtagttatt
     4741 attgatttta ttggaatata attgtatgaa gacttaaatt tttataaact gatgtgtata
     4801 ttgaaaaaaa tattttcctg aatgtagtta tagcatctta tgaaaggtgt aaattgtaat
     4861 ttaaaatttt tagtgtataa taagaattga aaattcaaac cgataaaaat taataaaaaa
     4921 taattataaa ttaataattt gggggaggaa atgtatgata aaattaatag caacagattt
     4981 agatggaact ttgttagatg aaaagagcga aataaatcca gagttttata aagtatttaa
     5041 aaaattaaga gagagaggga taatgttttc tgctgctagt ggcaggcagt atcaaaatct
     5101 tataaaaaaa tttgaagata taaaagatga tatgatgttt atatcagaaa atggaacact
     5161 agtagtatat aaaggaaaag aaatattatc aaatccttta aataaagaat tagtaaatga
     5221 aatcatagag acaacaagaa gtataaaagg gaaaaaaatt gtaatgagtg ggaaaaaata
     5281 cgcttatata gaaagcaaag atgaagcgtt tatacaagag gttagtactt attatgcaaa
     5341 gtttaaaatt gtagacgatt tgacgaaagt agaaggcgat atattgaaga tagctgtatt
     5401 tgacttcaaa ggtgcagaac ataataataa tatctacttt gaaaagtttt ctgatagagc
     5461 tcaagtatgt atttctggag tggagtggct tgatttaact gcaaaaggtg caaataaagg
     5521 gagtgccata aaaaaagtac aaaaaatgtt agatataaaa tatgaagaaa ctatggtatt
     5581 tggtgaccag ttaaatgatg tggaaatgat gaaaagtgcc tatcatagtt atgccatgga
     5641 aaatgcaaat gaacatttga aacaaattgc tagatttaga gcaaaaagaa atactgaaaa
     5701 tggcgttgta gataagataa aagaggtcat aaaaattgga tagtcatatg tatagatgaa
     5761 aaagattatt ttgaagaaat caaaaaaata tcaatgtaat tttgataaat aagtttattt
     5821 atgcgaaaat tacattgata tttaataaac atcaataata gatatttatt tttttatgat
     5881 attcagtatt ttgttttcta tttgagttgc attttcagca gaaattaaat aaacatatct
     5941 acctgctttc tcaattatag tattatttgc tatttcagaa ttgatttttt ctccatgtcc
     6001 ttctccaaaa ggtgcttgta tcatatcttt tttataattt tctagagttt tatatatttt
     6061 atctacctcg tcagttttta taacgataat atcttctaga gataattttt cagaagaatt
     6121 aattacaaaa ccttgaatta tgtcttcctc tacatcatta aaatagttaa agtcctttgc
     6181 atcaaagatt tttgattctt ttataagtaa atcagaagat tttaatgctt tttttatttt
     6241 tgatgtagaa acgtttttgt aatcaccact tgaagaacaa ctaaccattc ctaatgataa
     6301 gattatcgaa aaaaacaata ttaaaatttt tttcatgata aacctccaag ttttttaaac
     6361 tctgtcttaa attagtttac tacataatta tataaaaata aatgtattta atgttacagt
     6421 tttgattaat aatgtaatat tttgataata atacctattg agaggtgatt agaaatgaga
     6481 ataccaaaac atgtaggaat tataccggat ggaaatagaa ggtgggcaag tcaaaatggt
     6541 atgacaaaag ataaaggata tgaaagtgga atagaccctg gattagagtc ttatagatta
     6601 tgtagagatt taggctgtga agaggtgact ttttatggat ttacaactga taatacaaga
     6661 cgtccatcag tgcagacaaa agcttttaca aaagcatgta ttgatgctgc aaatatatta
     6721 atagatgaag gtgctgcttt attagttgta ggaaatacaa aatctccaat gtttccagaa
     6781 gaattattac catatactaa tagaattaaa gccaaagaag gtcaaataaa agcaaatctt
     6841 ctggtaaatt atgggtggta ttgggactta aataatatat caggagtaga aaatgtaaat
     6901 aaaaataata tacaggatta catacagtca tttgatgtat ccagactaga tttaataatc
     6961 agatggggtg gaagaaggag attaagtggt ttccttcctg tacaatcaat ttatgcagat
     7021 ttttatgtta tagataatta ttggccagat tttaagagtg acgatataaa ggacgcattt
     7081 agttggtatg atacacaaga tgtcacttta ggtggataat taaaaatatt ctgatttaag
     7141 gttgtagagt gttttaaatt attgaaaaaa aaacttcttg atggtaaaat aacactaata
     7201 atatgtaaaa gtgtaaaaat ttataccaag aagggtgaag aaatgtcaaa aggcaataat
     7261 aacaataatt caagaaacaa atctaaaaag accagtcatc tgaatagaaa aaagcgaaaa
     7321 ttaaataaga aaaagttagc agtgttgatt tgctttacag ttttattttt atttattgca
     7381 tttaaggcta ctcaaggtgt agtagctttg gtaaaaagta tggataaaag taataaaact
     7441 agtcaacaac aaaaagttaa ctcagaacaa tttgaccttg gtaatgaaga ggaaaataaa
     7501 aagaaaaaat atactgtatt tatagaccca ggtcatggag gcaatgataa aggaactgaa
     7561 agcaagacca gtaatagata tgaaaaagac ctaaatctac aaattgcaaa aaaattagca
     7621 aataaattat ctaaacaaaa ggatattcaa gtagtagtat ccagaactga tgatacatat
     7681 atatcattaa aggatagagc tatcttagca aataatagtt ctgctgatgt gcttgtttct
     7741 attcatttaa atgcagagaa aaatggtaat acagcaacag gtatagaaac ttggtatagg
     7801 aataaagcta cagatggttc aaaagaactt gcacagacag tacaaagtac tatagtatcc
     7861 tatgttaaag ttagagatag gggaatagtt gaaaataact ttgaagtact tagagaatca
     7921 aatatgcctg ctatattaat agagtgtggt tttttaacaa caccttcaga ggaacaaaaa
     7981 ataataaatg aaaaatatca agaccaactt gcggaaggaa ttgttcaagg tgtactttca
     8041 tacctagatt ctaaagggaa taagtaagtt aactttactt taatgtgagg ataaatttaa
     8101 aagtttaact gtgtaaattg tattaacttt atttcgtaat gttggaaaca ttgtttgaat
     8161 ttagaaacta tgaaattaga aattatttta tctatatcta atttcatagt tttttttgca
     8221 aaaaatttat agttataaaa tttaaaaaaa taaatttagt ataatgtaat gtagttattt
     8281 atatgtgatt ttaacttgca ttgatttgct atcaattgtg ctttataatt agtatatata
     8341 aagtttaact gtaagataat ttaaaaaata ggggaattta tatgaataaa aatagcatga
     8401 caaaaattta cagtttaaat aaaataattt ttattataat tggaatttta ggaatagttt
     8461 t
//
LOCUS       DAMKPG010000131         8441 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMKPG010000131 DAMKPG010000000
VERSION     DAMKPG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.136"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            224..1210
                     /locus_tag="PC621_003306"
     CDS             224..1210
                     /locus_tag="PC621_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF4110436.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1433..1516
                     /locus_tag="PC621_003307"
                     /pseudo
     CDS             1433..1516
                     /locus_tag="PC621_003307"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1547..3028
                     /locus_tag="PC621_003308"
     CDS             1547..3028
                     /locus_tag="PC621_003308"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF4110437.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3054..4025
                     /locus_tag="PC621_003309"
     CDS             3054..4025
                     /locus_tag="PC621_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF4110438.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4281..4883)
                     /locus_tag="PC621_003310"
     CDS             complement(4281..4883)
                     /locus_tag="PC621_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF4110439.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            5164..6375
                     /locus_tag="PC621_003311"
     CDS             5164..6375
                     /locus_tag="PC621_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF4110440.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6443..6640
                     /locus_tag="PC621_003312"
     CDS             6443..6640
                     /locus_tag="PC621_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF4110441.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6674..7645
                     /locus_tag="PC621_003313"
     CDS             6674..7645
                     /locus_tag="PC621_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4110442.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7832..8209
                     /locus_tag="PC621_003314"
     CDS             7832..8209
                     /locus_tag="PC621_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4110443.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3430 a          873 c         1358 g         2780 t
ORIGIN      
        1 aaattagagt atttatcaat tcatagtaga taaaattgaa attaaatata acaacaaaaa
       61 atgaaaatta caattaattt aatgttaaag actcaattat ttggatgtgg aatggatagt
      121 acaagtagta tataaaaagt attgactgga caaataacta tattttattt taaatatata
      181 atataatgta ataaaatata tcaaatgtag aaggtgataa cctatgaaaa aaataacaat
      241 aaatgatatt gctaacttgg caggagtatc taaaagtact gtatcaagat atctaaataa
      301 taaagatatc agtgattcta ctaaagagaa aataaagaca ataatagatg aatatggata
      361 tgagccgaat gcatttgctc aaagtttgag agctaaaaaa acatacttta ttggtattat
      421 aacaccgtgt ttagattctt ttgtaaaatc taaaattatg atggcaatag atgaagaact
      481 caaagaatta aaatatacat cactaattat taatactaat agaaaaataa ggtcagaaat
      541 agatagtata tcaaagttag ctagtctaaa agttgatgga ataatactaa ttggtaccga
      601 aataactaaa gaacataaga atgaaattga aaagttagat ataccaatgg ttgtagttgg
      661 acaaaaagtt gatggtatca atagcatagt gaatgatgat tatggagcag gatataaaat
      721 gggtcaatat attgctaata aaggttacaa aaacattgta tatttaggtg ttgatgaaag
      781 tgatatatca gttggtttaa atagaaaaaa tggagtactt aatgggttaa aagataaagg
      841 gtatgatgca aaagtatttt atactgactt tgaccaagaa acgtctatac aaagaagtgg
      901 agaaatgcta gaaagtgaaa atccagatat aataatatgt gcaactgata atatagcaat
      961 agcaacaatg aaagaaatca ataagagagg taaaaatata ccacaagata tatctgtagc
     1021 tggatttggg ggatatgatg tattgtctat tataagccct aaacttacaa caataaagtt
     1081 tgaaaataaa aatgcaggta aagtagcagc aaatacgata gtgaacttaa tacaagaaag
     1141 aaaagaacca ctattaaagg agattaaatt tgaattaata gaaggtgaaa gtacaataaa
     1201 taaaaattaa gatacacatt acatatatta gtaatgtgta tttttttact aaaaaaacta
     1261 taattctaaa aattaggaaa acttttttga aagaaatatt gcatctgaaa taaatttatg
     1321 ctaatcttta attaaaccgg tttcgaaacc ggtttaatta aaccgaaaga atagcacaat
     1381 gagtgcaaaa ataactgatt gtaacaaagt atcaaagtaa ttattagagt acataggaaa
     1441 taaacaaaat ataaaaggtg tagcacattg tgtaacaaga ttaagaattg ttttagatag
     1501 taataataaa gcaaatataa aattacaaaa gaaaaggaat aaaaatatgt acaaagagcc
     1561 taaatataga accatattag aatcgataaa agaagaatta aataaattaa gaaatatatc
     1621 tttaaatgat aaatataaac cactatttca tatacatcca caacatggac tacttaatga
     1681 tccaaatgga ttagcatatt ataatggtaa gtaccatgta ttttatcaat ggtatccata
     1741 tgatgcaact catggaatga agcattgggc ttatgtaagt tctgatgatt ttgtaaactg
     1801 gaatagagaa gatgtagcat taataccaat cgaaagttat gaatctcatg gagcatattc
     1861 aggtaatgcg atagaggtag atggaaaatt gcatatgtat tatacaggga atataaaata
     1921 tagtgcagaa gatagatatg catatcaaaa cttagctata atgaacaagg atggtaaaat
     1981 aactaaatat gaaaataatc caatagtaag tgaaatacca aaaggatata caggacatgt
     2041 aagagaccca aaagtattta aaagaaagga taaatacttt atgttacttg gagcacaaac
     2101 aagtgataaa aaaggagtta taatagtata tgaatctaaa aactctatag attggaattt
     2161 taaaggagaa ttaaatgtaa aaaatataga tgaagatttt ggttacatgt gggaatgtcc
     2221 agattatatt aatatagatg aaaaagatat attaatattt tcgccacaag gagtagaacc
     2281 taaaggattt gattatcaaa atatatataa tgtagtatat gctattggta atatggattt
     2341 agataattta acatttgaaa tagatacaat gaaagaactg gaaaaaggat ttgatttcta
     2401 tgctccacaa acatttataa aggactcaca aataatacta tttgcatggg ctggaatggg
     2461 agaagtttta taccctacag ataaaaacaa atgggcacat tgtctaactg ttccaagaaa
     2521 attaaatata aaaaataaca agctattgca aatgcctgta gatgaattaa ttaaattaag
     2581 atatgatgaa acaagtgggc aaaatactat aaagaataat atcaatatta tagaaaatga
     2641 tgaaaatgta tatgagctta atattaatat aaaaaatatt gacagcaata aatttgggct
     2701 ggaattattt tcatcacaag atgaaggtgt aaaattagaa tttaacaaat taggaaacat
     2761 tgtaacttta gacagaagca actttaaaaa agttttcggt gttgaatatg gaacaaatag
     2821 aaaagaatat ataaatattg atgaaaatac taatatcaaa gtactagcgg atagaagtgt
     2881 tttagaaatt tttataaatg atggagaagt agtatttact agtagaatat ttgctaaaga
     2941 aaattctaat caaataaggg tttatagtga taaaatagta gagtatgagt atactaaatt
     3001 taaattaaag caaggtattg aattataaaa acacattata aggggtagaa aagatgaaaa
     3061 aagtaattag tataggagaa gctttaatag actttatacc taatcaaaat ggttgtaaat
     3121 taaaaaatgt atcagaattt agaagagtag caggaggagc tccagcaaat gttagtgttg
     3181 ttgtagcaaa gcttggagga aaatcaagtt ttatatctaa gttaggaaaa gatgcatttg
     3241 gtgattatat aatagatgta ttaaatgaag taaatgtaaa tactgattat gtattgagaa
     3301 caagtaaagc aaatacagga cttgcttttg tatcattaaa agaagatggt aatcgtgatt
     3361 tctcatttta tagaaatcca agtgcagata tgcttttaga agctgatgag gttaaaaaag
     3421 agtggtttaa taattgtcat atcttacatt tttgttcagt agatttaata gatagtccaa
     3481 tgaaattagc acataaaaaa gcaatagaat acgcacttga aagtaatagt ataataagtt
     3541 ttgaccctaa tataagatta cctttatggg atagtgagca atcttgtaaa aaggcaatat
     3601 cagaattttt accttttgct catattgtta agatatctga tgaagaatta gaatttatga
     3661 caggagaaaa tgatatagaa aaatcattac ataaactctt tgtggggaat gttgaactag
     3721 tattatatac aaaaggaaaa gatggagtag atgcatacac taaaaaagta aaaggtatgt
     3781 gtaaaggggt taaagtaaat gcaatagata caaccggagc aggagattct tatataggtt
     3841 catttttata caccttattg tataaacaaa taacattgaa tgatataaaa gaaatgaaac
     3901 aagaaacgtt aaatgagtat ttagaatttt ctaattatta tgcagctcgt agtacaacta
     3961 ttaaaggtgc gataagttca tatgcaacaa aggaagagat aactgaattt ataaaaaatt
     4021 tatagtagga atgagtaata ggttttgaag gaagtaacat gtaaagagtt tttaaggatg
     4081 tttactatga aaataattaa catataaaaa gcaataaaga ggctgtctca aaatagatat
     4141 aagcctcttt atcattgttg aaatgataaa aaatatttta attattgtta ctttttagaa
     4201 gaatatatat cttattttaa ataagcaaat tatgtaagat ttatttatat tatcaatact
     4261 ttacttggag tattatgtct ttagcagtta tctacttcaa ttgaattatc taaaatataa
     4321 taaatttgtt ttgcaaattg acgagaatgt ttttcttttt ctttattcat aaaattaggt
     4381 ttacctaaat cacgttcaac aagcattcct atatatttta actgtgaatg cttacagtag
     4441 cgcttcatac cattttccca aatatcagca cccttttcag gtctatatcc acatgttgta
     4501 ataagtgcta ctggttttcc tgcccataga gcggggcctt tctcattacc atagtactta
     4561 ttcattccat acaccaatct gtcaagtagt gctttcattg gagaagtgca gtaccaagaa
     4621 taaattggag tagcaaaaac aattaaatca cacataagaa ttttcttgaa aattttctga
     4681 acatcatctt tacaataaca tccaaaatca aataagttat cctgacattt tttacaagca
     4741 atacatggct ctattttaca atcatataac catattactt caatatttga attataattt
     4801 tctaattcct caataaaagg ttttaaaata gcagcagtat tacctttttt cttaggactt
     4861 cccataagaa tgcatatttc catattgata cctccacagt attttaaaat agtttttagt
     4921 gcttaatata gttttaggat actatgagaa tacgttaaca acaatacact ttgtatattt
     4981 ttaataaaat tgaaacaaat tgttttatca ataaaagttg gaatgtatct ataattaaaa
     5041 atattttagg tatgttttta catatatgtg tgtagttaac tatggtaatt tattgtaaaa
     5101 ttatcttata ggattataat tgcttaattt atgattatat ttaaaatctt ggaggtaaaa
     5161 aatatgtact caaatgaaga ctataaattt atagaagaaa atgcaccatg ttatatatat
     5221 gatgagacta atataattga aaaatgtaag gaattgaaaa ataatatttt aggttttgat
     5281 tttttatatt ctataaaaac aaatccatat aacaaaattg taaaaagcat tgcagagcaa
     5341 ggttttggag cagatgcttc ttcaactaat gaagttatga tttcatcaga atgtggagtt
     5401 tctaaatatg atatttatta ttcatcacca ggcaaaacag agattgatat agaaactgtt
     5461 ttagataaat gcataattat tgcagatagt attacagaaa tagaacttat aaataagata
     5521 tctaaaaagc ataatcagaa agttaaaata ggaataagaa taaatcctga ttttactatg
     5581 gatgatgata gtgctaaatc aagcaaattt ggtatagata tagaagatat gagcagtgtt
     5641 tttaagatgt taagtatgtg tgattatgta tctgtagttg ggattcatat acatattaaa
     5701 tctcaggtat tggattttga aaagttagga cattattatg aaaaatgttt ttttatagct
     5761 ttaaatttaa ataaaagtaa gtatattaat attgaattta taaattttgg tagtggactt
     5821 ggagtagctt atgataatct tagagaaaat ccacttaatc ttaaagagct ttcaaaatta
     5881 actcaaaaga ttgtttttac taattcaaac acactgaaat caagacttct tattgaatca
     5941 ggacgtttca tagtttgtaa atcaggtaat tattatgtta aaattgtaga tattaaagta
     6001 tcaagaggga aaaaatatct tattgtaaaa aatggactta atggatttat gagacctatt
     6061 atttctaatt taattgaaaa tattacttgt ggagtagaat tagaaggtca agagccatta
     6121 tatacatcaa atcatgcctt ttctctttca gtattaaata atttaaaaga agaagagtat
     6181 gtaaatgttg ttgggaatct ctgtacatca ttagatgtaa ttgcaaataa tgttaaattg
     6241 aaaaaagcaa atataggaga tttgatttca attagtaatg ctggaagtta ctcctattct
     6301 ttatcaccac tattattttc gagttatgaa tttcctaaac aatattataa aagaaaagat
     6361 ggatatattg agtaaacaat aggataaatt aataaactta gtattattga aaaaatttaa
     6421 aatgcagggg gtgtaattct atatggaatt gtggaaaaga aatttatttg tatgttggat
     6481 aggtatgttt tttagttcta tagggatgag ccaaatagca cctatacttc cattatatat
     6541 taaacagttg ggggtaactg atgttagttt aatacaacag tattcaggta taatttttgg
     6601 atgtaccttt gtagtagcag cattcttttc gccaatttga gggaaagctg ctgataaata
     6661 tggaagaaaa tcaatgcttt tacgtgcaag tattgggatg ggaatagtta tatttaccat
     6721 ggcgtttgtt caaaatgttt atcagttatt aggattaaga atacttcaag gagtttttac
     6781 gggttatgct actgcatgta ctactcttat tgcaacacag acagataaaa atcattcagg
     6841 atgggctttg ggtactcttg caactgcatc aactactggc tcattaattg gtccaacagt
     6901 aggaggatat atagaatcaa tcttgggtct taaaagtaca tttattataa caggaggatt
     6961 gttgtttgta tcatttatca tctctatttt atttgtagta gataatttta aacctaaaga
     7021 aataaaagaa tcaatttcta tacctaaaga acaattaaat atattgccaa ataaattttt
     7081 aattgcatct atttttgtaa ccacattcat tactcaacta gctttatact ctatagaacc
     7141 aattgtaacc atatatatat cacaacttac aaattttgca tcaaatgtag ctttaatagc
     7201 tggattgaca ttttctgcat caggattagc aaatttatta gcagctcaaa aacttggaaa
     7261 aatgtcagat aaaataggtc ctcaaaaagt attgttaatt tcacttttat gggctgggat
     7321 tatatttata ccacaagctt ttgtaaaaac tgcatggcaa ctgatgttgt tgagattttt
     7381 attagggttg tcagttgcag ggcttaatcc ttctgttaac agcttgctta aaaaaattgc
     7441 accagaagaa tatgtgggaa aaatatttgg atataatgca tctgcccagt atataggttg
     7501 ttcatcagga gcatttttag ggggacaaat atcagcacat ttaggtatta gaacagtatt
     7561 tttttcaaca agtttattgt tgtttatgaa tgcactttgg atgtataaat ttactggatt
     7621 gtttattaaa gataagacta aataatcata ctatttacat ttctaatttt atgatgtaaa
     7681 ataaatattt tttatgtaat ttatcataga attatataaa aaataactct atggtaaaaa
     7741 ttggaaaata ttattatcga caaaatcttt gacttaaaaa ctatattgcg atagtattaa
     7801 tttaataaaa gtttgagagg ggcgtaaggt tatgacaatt tcaaaaatac cacaagcaga
     7861 attaaaagtt atgaagttta tttgggaaaa aaatgataca gtaacatcaa aagaaactat
     7921 tgaagctatg gaacgaaaat atggttggaa acaaacaact acattgacac ttttatcaag
     7981 acttgttaaa aaacattttt taagtgcaga aaaaatagat agatacactc attatacaat
     8041 agttgttgga cataaggagt atttaagtgt tgagacaaaa gattttttaa gcaatataca
     8101 cgacaattct ttacagagtt taatttcagc actacatgat gatgaagttg ttgataaaga
     8161 tatgttggat ttatttgaaa gtcattttaa aaatttagaa gaagagtaat tatatcagga
     8221 taaaactcta agaatttaat taatctatgg ctgaaaacca taaatggtat tatttaatca
     8281 gctactcact attcataaag taatattaaa taggttctct aattagtaaa atacctaaaa
     8341 taattggaat agggagtatc aagttaaatg ataccactta tttaaataag aaatgatttt
     8401 taaaatttaa aataatttta taacttgtca atattcatta a
//
LOCUS       DAMKPG010000132         8412 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMKPG010000132 DAMKPG010000000
VERSION     DAMKPG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.137"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            272..1279
                     /gene="asrA"
                     /locus_tag="PC621_003315"
     CDS             272..1279
                     /gene="asrA"
                     /locus_tag="PC621_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF4110444.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            1280..2071
                     /gene="asrB"
                     /locus_tag="PC621_003316"
     CDS             1280..2071
                     /gene="asrB"
                     /locus_tag="PC621_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF4110445.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2084..3055
                     /gene="asrC"
                     /locus_tag="PC621_003317"
     CDS             2084..3055
                     /gene="asrC"
                     /locus_tag="PC621_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF4110446.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3112..3888
                     /locus_tag="PC621_003318"
     CDS             3112..3888
                     /locus_tag="PC621_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF4110447.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            4159..6177
                     /locus_tag="PC621_003319"
     CDS             4159..6177
                     /locus_tag="PC621_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF4110448.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            6392..7249
                     /locus_tag="PC621_003320"
     CDS             6392..7249
                     /locus_tag="PC621_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110449.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            7346..8215
                     /locus_tag="PC621_003321"
     CDS             7346..8215
                     /locus_tag="PC621_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4110450.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         3434 a          753 c         1513 g         2712 t
ORIGIN      
        1 ttgtataaaa aagcaaataa gattctttat tattcaaaaa attgagtaga taatagtttt
       61 attgtattta atgattgtat ctaagtgatt agatttgaaa tcgaataata tagataataa
      121 aaaaatatta acagagatta gtaaactcta ttaatatttt tttattattt ttgaaactgt
      181 gataattatc acagaagcat agaaaataaa gtgttaagat ttaaacaaga tagcaagtaa
      241 gtaaaactaa aatataagaa aggtgataaa aatgaaacta aaattagatt catctaagtt
      301 caatgaaggt ctgaattatc taaaaaaaga ctacaagata tttgcaccag ttttaatccc
      361 atttaaaggt actttttcag atactgatgt tataagatac aaggaggtaa acgcttttga
      421 agaaatggaa tttgctaaga aagcaaactt ttcaccaaaa gaagttgtac ttcccataaa
      481 tcaagtattg ttttatttta ctgaaaaaga gtttaaggaa agcgatttag acaacaagaa
      541 aattttaatt ttcttaagag cttgtgacat aaatggtata aaaagacttg atgaaatata
      601 tctaaataat ggtgaagaaa aagattattt ttataaaaat atcagagata aagtaaagtt
      661 tgcacttgta gggtgtaagg aaagttttag aaactgtttt tgtgtgagca tggattctaa
      721 caaaactgat aattattcaa ttggattaaa tatagaagga gatacactgt acttagatat
      781 aaaggatagt gaatttgaag tatttaatgg agaagcaact gaatttaatg ttgattatgt
      841 aactgataat ttaatatctg tagatgtgcc agagaatatt gattcaaatg aaattatagg
      901 aaattcaata tgggatgaat atgatacaag atgtatagga tgtggtagat gtaattttgt
      961 atgtccaaca tgttcatgtt ttacaatgca ggatattttc tataaggaaa acgaaaatgt
     1021 aggtgagaga agaagggttt gggcttcttg tcaagtagat ggatatacag atatagcagg
     1081 tggaaattct tttagaaaaa aacaaggtga aagaatgaga tttaaaacta tgcataaaat
     1141 acatgatttt aaaaaaagat tcggatatca tatgtgtgtt ggttgtggaa gatgtgatga
     1201 tgcctgtccg caatacatat cattttcaaa gtgtatagaa aaaataaatg atttagtaat
     1261 tagtaaggag gaagtataaa tgaacccata tataccagta agtgcaaaaa tactagatat
     1321 agttaaacat actgataaag aatggacttt tagagtaaac tgcaatacaa aaggtgtatt
     1381 gcctggaaag ttttatgaga tatctatacc taaatacggt gaaagtccaa tatcagtatc
     1441 aggatatgga gaggattata ttgactttac aataagaaat gtaggaaaag taactagcga
     1501 gttgtttaat tataaagaag gagattcttt ttttataaga ggtccttatg gaaatggatt
     1561 tgatgtatcc ctttatgaag gaagagaaat tgtagtagtg gcaggaggaa gtgcattagc
     1621 ccctgtaaga ggaatagttg aatattttta taataataaa gaaaaatgcg aaaagtttaa
     1681 attaatagta ggatttaaat caccaaaaga tattttattt gcagatgatt taaagagatg
     1741 gagtaaaaaa cttgatattt tagttactgt agatggagca gaggaaggat atagtggtaa
     1801 tgtaggatta gtaacaaaat acataccaga attggatatg aaagatataa acaatacttc
     1861 tatagttgta gttggacctc caatgatgat gaaatttaca gtagaagaat ttttaaaaag
     1921 agatttagat gaaaagaaca tttgggtttc ttatgaaaga aaaatgtgtt gtggaatagg
     1981 taaatgtgga cattgtaaaa tggatgatac ttacatatgt ttagatggac cagtatttga
     2041 ttattcatat gctaaaaatc ttattgatta gggggcgaag tttgtgataa gagatttaaa
     2101 taccaaaaaa gtaatgaaaa atgcttatag aattacaaaa acaaaatatg agacttcttt
     2161 aagagttaga gtacctggtg gtcttattga ccctgagagt ttagctgtag tttctaaaat
     2221 tgcaagtgag tacggaaatg gacaggtgca cataactaca agacaggggt ttgaaatatt
     2281 aggaataaat atggaagata tggaagaaat aaataaaatt atacaacctg ttattgaaaa
     2341 aatgaatata aatcaaagtg agaaaaacgc aggttatcca gcagcaggta caagaaatat
     2401 agcagcttgt ataggaaata aagtctgtcc aaaggctcaa tataatacaa ctgaatttgc
     2461 taaaagaata gaaaaagcta tatttccaaa tgatttacat tttaaagtgg ctttaactgg
     2521 ttgtccaaat gattgtataa aagctagaat gaatgacttt ggaattattg gtatggcttt
     2581 accaatatat gaaaaagata gatgtgtaaa ttgtggagca tgtgtaaaaa aatgttctaa
     2641 aatatctgtt ggagcattaa aaacagaaaa taataaggtg gtaagagata aagataagtg
     2701 tataggatgt ggagagtgtg tactaaattg cccaacaaat gcatggacta gagatgagaa
     2761 aaaatactat agattagcta taatgggtag aacaggaaag aaaaacccta gactagcaga
     2821 agattttctt ttatgggtag atgaggacag cataataaag ataatactta atacttatag
     2881 atatgtagaa aagtatatag ataaagatgc accaggagga aaagaacata taggatatat
     2941 aattgataga acaggattta tggaatttaa aaaatgggca cttgaaggtg tagagcttcc
     3001 agaaataact aaaatgtacg aaaacatata ctggagtgga attaaatatc tataaattga
     3061 gtaaaacata tatttgaata aattaaaatt taatagaagg aagagggaat tatgcataaa
     3121 gaaactttgg ataaattaac taatgcagct ataaataaga taaatttatt aaacacaagt
     3181 aaagtaaaat atttggtatc ttctgcgttt gcaggacttt atgtaggtat aggtatactt
     3241 ttgatattta ctataggagg acttttgact gatgcaggtt cacctatgac taagatagta
     3301 atggggttat catttgcaat agcactaagt ttggttataa tgacgggaac agagttattt
     3361 actggaaaca atatggttat gtctgctggt atgttaaata aaggtgtaag tataaaagat
     3421 acttctaaaa tatgggcata cagttgggta ggtaatttaa tcggagcttt ggttttaggt
     3481 cttatatttg taggaactgg tctagtagac aaaggacctg tagctgaatt ttttgcaaat
     3541 acagcagcta gtaaagcatc catgccattt acagctcttt tctttagagg gattttatgt
     3601 aatattctag tatgtgtatc agtgttgtgt tcgtttagaa ctaatagtga tactgcaaaa
     3661 ataattatga tattcttatg tttatttgct tttataacat caggttttga gcatagtgta
     3721 gcaaatatga caatttatag tgtttcatta ttttcaccaa caataagtac agttactatt
     3781 ggaggagcta tttataattt agtagctgta acacttggaa atattgttgg aggagcttta
     3841 tttatgggtc ttggaactta tatattaggt aaagaaaagc ttaattaaat ttattgtata
     3901 aattataact ctcttgataa atacattttt agtattattg agagagttat ttttatattt
     3961 ttgaaaaaat atttgaaaca aaaaaaatat gggagcatac gaaaatatat cggttatata
     4021 taaaaaaaat acgtttatat atgaattata tagttctaaa tataaaaaaa gttaaagtcc
     4081 taccgtaaaa aagcgcattt tgaataagat aacaaatatg ataaaattgg tcttgttgaa
     4141 atttttaggg gggatatgat gacaaagaaa ctagtaatat caaaaacaaa gttgtttatt
     4201 tttataatag catttatttc tatatttata tatttatttg gaagtaaaaa tacacttata
     4261 ggtgtaggta tagtaacagc tatgctaaca ctcttggaaa gagatttaac tatttctcca
     4321 attaaaaatt tactgaagta tttagcaata aatataatct taggaatact atcatttttt
     4381 gcagtacaaa atatgtattt aggagtttta ttaaatttta tagcattatt tataataggg
     4441 tatgtattta gttatgactt aaagagagca gtatatgtac cttttggtct aatgtatata
     4501 tttatggtaa gtatacctgt aggaaaaagt gaatttccta tgagattgtc ggctttagct
     4561 gttggagcag ttataataat gattgctcaa tttgttatga atagaaatag gatgaaaaat
     4621 gtcggagata aagaactgat atctatatgt gatgaattat tagaaaaaat tagtctttta
     4681 aaaaatacta ttaatgatga tagttctata attaaatcta gtatgagaaa aatagatagc
     4741 tgtaattata gaataaattc tatatctaaa aatttgaaaa tggttatttt tgataataga
     4801 aaagatgact tttatatcag tattagaggt atagacataa tgaatatttt attttcatta
     4861 gaaagaataa gtttaatcat ggaaagatac aaaaaggata gtaaagaatt tgaagatgaa
     4921 gatataaaaa atattttaga agaaagtagc ataaatagta aatcagatgt tctagttgta
     4981 gcaacgaaag aaataaacta tattaagatg tgtttagaaa ataaagatac cataactgat
     5041 aaagagatat taggatttag agattatgtt attgctcagg atactaaaaa tataaattta
     5101 aaagaaattt atagtgtttt agaaaattta tatgagtttt tagtagatta taaaaaagta
     5161 aaatcaagag acgaaaagaa agctgaaaga aaatcaaaga tacctcatga atttaaacga
     5221 ctatcaatat ataagaaaaa ctttaattta aattctataa gattttctta tgcagtaaag
     5281 attgctttag ctacagctgt agcgggtttt attatggatt atttccattt aagagatgga
     5341 agatggataa tgttaactgt cttttcatta acacaacctt acgcagaaaa ttgtatacaa
     5401 aggtctagaa aaagaataga aggtacattt ataggagctg taatatttat tgtattattt
     5461 tcaataataa aagatagtac attaaggtcg ttaatagttc ttttagcagg gtatataaac
     5521 tcttatgtag ttgattatag aaaattaatg gtatgtgtaa ctgtttcagc tcttggttca
     5581 gcagtagtta tgggagaccc aaatgtttta accataagca ggatttcata tgtagcatta
     5641 ggagcaatta ttgcattaat agtaaataag tttatattac catatgatgc taaaacaggc
     5701 tatcaacatg ttatagaaat gtataaagga atagttaaaa atataattga tgaagtcaat
     5761 aagtctatag aaaatgtagc tgatgtttac tatataaaaa atttattatt gataccatca
     5821 cttattgaag atagacttat gttgataaat gctatatata aagataaaca tcaagaggat
     5881 tttttagaaa atcagaaatt actaataagt aatatgtata atctatatat aaatgttaat
     5941 aaaaataaaa taaaagatga agatgttgaa aaaatattga gagatactaa ttatatttca
     6001 aattacaatg tagataagta tgatgaagga agaagtgtaa ttttagagag tattgtaaat
     6061 actaaaagtc taggtgacaa gataatatgt ctaaacttac tgcaaactct aaatggtgtc
     6121 aaagaaatgt atagaataag taatataact aaagtttcaa taaaagaagc agcttagata
     6181 ttaaattata gaatagccat atatttaaaa taaagtatat ggctatttgt tttataaaat
     6241 actaaaagaa aataatatat aaaaaacaaa aaacatatat ttatgaattt tatatgaaaa
     6301 tagttaaaat attgattttt ttcattggtt gaaaaaacaa tttacttagg ataaactata
     6361 atagtattca aatattatgg gggttagatt tatgagtaaa gatgtaaagg taaagcgtaa
     6421 taagaaatat gatacattta gtataactat ggaacttgag agacctttac tttatgatga
     6481 ggttgagaac aaagatgata aaaataataa tagaaaattt aacataaaga ataagatatt
     6541 taagggaata ggtatactat ttgtattgat tttgcttggc tttttcatat ggataaataa
     6601 aacatatgaa cctcaaaagc tggcaaaaga agctctagtc agcaatagta aagttgaagt
     6661 aactgtaaat gaaaacattt catttacacc aaaaggaaga aaagtttcta aaggtttaat
     6721 tttgtatcca ggagctaaag tagaaataga atcttatgct ccacttgcaa gaaaaatagc
     6781 agaaagtgga tatgaggttg taatcgtaaa gatgccactt aatctagcta tacttggaac
     6841 taataaggca cagaaagtta tggattctta caacaacata gaacattggg taataggagg
     6901 tcattcatta ggaggagttg cagcatcaaa ttttgcacgt gataataaat taattgaagg
     6961 cgttgtattt ttagcttctt atccaatggg tgatgagttg aaagaattag gaaaaaaagt
     7021 tatatctata tgggggagca aagatggagt agtcaatttt aaaagtcttg tagaatcaaa
     7081 acaaaaactt cctgatgata caacttatgt tgagattgaa ggaggaaatc atgcacaatt
     7141 tggagactat ggaaaacaaa agggagataa tgatgctatt ataagtcaag aaaaacaact
     7201 taatattact gccaatagta tcattaaatt tttaaagaat atatcttaaa ctaaaattta
     7261 aaaaatataa ttttataagt tattatttaa agtaaatatt aataatataa tagaatgaat
     7321 attttattac aagggggatt ttaatatgga atacgatatg ccactttaca gaccaccaag
     7381 tgaggcttat agtcttataa tacaagtcac actaggatgt tctcataaca aatgtacgtt
     7441 ttgtagtatg tacaaatcta aaaaatttaa aataaagcca ctagaagtaa taaaaaatga
     7501 aattgatata tttagaagat attataagca tgtagaaaga atattcttag cagatggaga
     7561 tgcattgata ataccaatgg agaaattaag agatattatt ttatatataa aagaagtttt
     7621 tccagagtgt gaaagaatta ctatgtacgg ctctcctaaa tctatagaga aaaaatctga
     7681 tgatgaactt aaagaattaa gaaaacttgg tgttaaaatg atttatctag ggcttgaaag
     7741 tggaagcaat gaagtattag aagatattaa gaaaggtttt agtagtgaac aattaataaa
     7801 agttggtaga aaagttaaaa aaagtgggat taaattatca gccactgtaa tagcaggtct
     7861 tggaggaact aaaaaaacac atcagcatgc agttgataca ggaaaaatgt taggagcaat
     7921 ttcaccagat tatgtgggtg tacttagttt aatggtagaa cctaatactg aactttacga
     7981 tttacttaag gcaggagagt ttacaatact agaggataag gctgtgttac aagaaattaa
     8041 agagatgata aagaatatag atgctaatga aaaaattgta tttagaagta atcatgcatc
     8101 aaattatgct aatttaaaag gtgtattacc tgatgataaa caacgtctaa ttgatgaaat
     8161 tgattactat ctaagtaacc aaaagctaaa aagagaagaa tatagaagac tatagttttt
     8221 gatatatcaa tctatattga taggtaaata ctaagattat ttagatacaa aaaatcatgc
     8281 tacattaata gttgcttact actgatgtag catgattttt ttattaaaaa acaaaatggt
     8341 atattcatat ataatatatc aataataatg aaaatgtgaa ctagtttaat agaattttac
     8401 ctattataat aa
//
LOCUS       DAMKPG010000133         8391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMKPG010000133 DAMKPG010000000
VERSION     DAMKPG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.138"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            278..1720
                     /locus_tag="PC621_003322"
     CDS             278..1720
                     /locus_tag="PC621_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF4110451.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1762..2115
                     /locus_tag="PC621_003323"
     CDS             1762..2115
                     /locus_tag="PC621_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110452.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2306..3127
                     /locus_tag="PC621_003324"
     CDS             2306..3127
                     /locus_tag="PC621_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF4110453.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
     gene            3385..5268
                     /locus_tag="PC621_003325"
     CDS             3385..5268
                     /locus_tag="PC621_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF4110454.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            5492..6172
                     /gene="pgmB"
                     /locus_tag="PC621_003326"
     CDS             5492..6172
                     /gene="pgmB"
                     /locus_tag="PC621_003326"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF4110455.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            6169..>8391
                     /locus_tag="PC621_003327"
     CDS             6169..>8391
                     /locus_tag="PC621_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF4110456.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     P"
BASE COUNT         3384 a          788 c         1521 g         2698 t
ORIGIN      
        1 ttcattttgt aaaacagtga attaaatatg taatatagat atcgattttt attacaaatt
       61 gttactaaat ataggtacat tagaacaaaa atctagtaaa ttgtaactaa aatgtaaatt
      121 tttcctttaa tctatacaat attatggaaa ctttttgtaa tttatgatat aataacaaat
      181 atataattat taaattataa taaatgacta tagcgtagat gtaatactat gttatagtca
      241 tttttgaatt aaacttgata tatcaagggg gaagaatatg agaacaatta gaagagtttt
      301 agcattaggt ttaacattag ctatatttct aataaatgta cctaatgtag atgcattaac
      361 atcagataca attaaaggta acaatatata tgagacggct ggattaatag cagataaaaa
      421 gagttatgat acagcaatta tggtaaatat ggataattct atagcagatg gtctttcagc
      481 aagtggtctt gcaggtgctg ttgatgctcc aattttgctt gcacaaaaaa ataagatacc
      541 aaatgaaaca aaacaaagat taaaaaatgt taaaaagata tacataatag gtaaagaatt
      601 atcaataagt aagtcagtag aaactgaact aaaaaacaca ggggcacagg taactagatt
      661 gggtggagat gacagaataa agactagtta tagtgttgct aaagaggtaa atggtatcaa
      721 aaaagttgat gaagtgatat taactaatgc atataaagga gaagcagata caataagtgc
      781 tgcacccgta tcagtaagag atatagctcc tatagtactt acagatggaa agagtgtgcc
      841 tttttcaaca agcaatgtaa aaacatatgc agtaggtggg agtatatcaa tgagcactag
      901 tttagttaat aaaactaatg ctaaaagact tggtggttca gatagatatg atacaaataa
      961 gaaagttata aaagagtttt atccagatgc atcagaattt tatttgagtg atggatatga
     1021 tttagtaaat gcacttacag gttctacaat tgctaaggaa aatccaattg tattggtgtc
     1081 agaaagtagt gataagtcta tattagcagg agcagataag attactagat taggttcaat
     1141 aagtgacagt gtgtataata agtgtgtttc tgctgcacaa aataatggcg attcgtctac
     1201 aaaaggtgag tcacctatga aaaatgagac aagcatatta ggtcagccaa cagctagttt
     1261 agaagcgtgc ttaaaatggg caaaatctaa aaaagctaat gatttattta tagagttaat
     1321 accaatatta tatgatactg ctgttcagga aggtgttaat cctgttttgg cagtagctca
     1381 atctgcaaaa gaaactggtt tttgtaattt tggtggagta ttagatgcat catttaagaa
     1441 tccttgtgga cttaaaactt ctgtaggtgg ttctgatact gataagaatg cccattcaag
     1501 atttgatact tgggaagaag gaatattagc tcaaattcaa catttatgct tatatgcagg
     1561 acaagatggt tacccacttt caaatccagt agaccctaga catgaaaaat ctttatttgg
     1621 taaggctaaa acagttgaaa gtttatctaa taattgggct ggaggtcaat atggacaaga
     1681 tttagtaaga atgatgggag aaattgaggc aacaaaataa ttgaagatta tcaaatatcc
     1741 aataaataag gattgattat tatgaaaaag agtaatattt ttagtatcat atttcctaca
     1801 atcataatgg tgctaatgtt tttaactttt gctacagata tatttaatat tccagatatt
     1861 caatctaaaa gtatttttgt tgtgggaatg gttttgattt ttccaatttc ttttttaata
     1921 caagggataa tttgtgtatt aaataagact aattggattt tatctcttat tgtgtcttta
     1981 gtaacttata tagtattaat gtatatattt ttaaatgatt ctgcatatat atatgtttta
     2041 ctttatacag ctttttatat gattggacat ataacagcta aactctatta caaaattaag
     2101 acttttaaaa attaaattga agtatataaa agtaacatta agagaagtat ccttaatgat
     2161 tttctaaaat cagaaatagg tgcttctctt ttttgtaaaa ataaaagtat gtaaataaaa
     2221 tttaaccatt ataattttct tttaacaaat gtatacaata aacttgttgt ataattaaga
     2281 tagataaaat aaagggaggg ataatatgaa tttaatagat aatcatattc atactaattt
     2341 ttcaagtgat ggaaaggatt ccatggaaga tacaataaaa aaagctataa gtattggtgt
     2401 tagatatttg acttttacag accatttaga acatgatgaa gatagaggat tttgcataaa
     2461 ttataatgac tatgttccag tatttaataa gtttaaagaa aaatataaaa aggatataga
     2521 gttattacta ggtgttgaag taggttacag aaaacatcta aaaaatgaaa tagaagaaat
     2581 aataaattct aatccttttg attttgtatt gtgctcaact catacaattg acaatgtacc
     2641 tgttccatca aaggcgtatt ttaaaggtct tagcaaagaa gatgcgtatt ataaatattt
     2701 taatagcata cttgaaacta atcgtgaatt tggagattac aatatatatg gtcatttgga
     2761 ttatatatct aggtatggaa tatattcaga taatagagtt atatacaatg actttaaaga
     2821 tgttattgat gaggtcttaa agtcaataat caataatggg agtggtatag agctaaatac
     2881 ttctggttat agatatggat taaatgctat acatccaaat gaagatattc taaaaagata
     2941 taaagaactt ggaggattta tagttactgt aggttctgat tctcataggg tagaagatat
     3001 atgtaaagat tttgatgtgg cctatgatat gcttaagtat cttgacttta aatatgtatc
     3061 tttatttaaa gaaagagaaa cttattttgt aaatataaaa aaagttaaat ctaacaatat
     3121 agcttagagt atgttttgaa gtaaattaat ggatagttaa aaagttaata gttataatat
     3181 tgtatcctct agaaaaatac tagaggtttt tattttgcta aaataactaa atagaattaa
     3241 atttaaatag tatagtttat tttaaattga atagtttatt gttgatatat tttactatta
     3301 tatattttta ttgtgatata atagtaaaat atattaacct atctttagat tatataaatt
     3361 attataaagg agggaaattt taaaatgttt aataaaaata aagaaaatat aactttaagt
     3421 aatcaaatca aaggaacaat tcaaaatagt ttaacaaaag aagctatatt acatatacca
     3481 atgagcaatt atgcctatgg ttatgataga gaaacactac atataagact tagaactaaa
     3541 aaaaatgaag cgaaaagagt aattttacga ataggagacc aatatgtgtg ggataaagga
     3601 ggtgcaggag gaggaaattt aaatgcatct ggagttggtt ggtctggtgg aacaaatata
     3661 gtaatgagta aagaagtaga aactgaatta tttgactatt ggatagctaa gtgtaagcct
     3721 ttaaataaac gctcacgata tggttttata atagaaggtc aagaagaaaa acttttattt
     3781 acagaaaaaa gaataataga gttgggaaat gaaaatgatg aaaaggaatt atgtgaaata
     3841 ggtaattttt ttggatatcc atatttaaat tatatagata tacctaaagt gccaaattgg
     3901 gtgaaagata ctgtttggta tcagatattt ccagatagat ttgcaaatgg tgacccgtct
     3961 ataaacccag aaggagtaga taaatgggga gctattccaa ccagagataa ttttacaggt
     4021 ggagatttac aaggtgtgat agaacactta gattatttga gtgatttggg aataaacgga
     4081 atttactttt gccctattac aattggaaaa accaatcata gatatgatac ggtagattat
     4141 atggaggtag accctacatt aggagacaaa gagactttaa aaaaactaat agaagaagcg
     4201 cataagagaa atataaaaat aatgttggat gctgttttta atcatatagg atattattct
     4261 aaacagtggc aagatgtagt tcaaaataag gaaaattctc gatataaaaa ctggttttat
     4321 ataaaagata tgagtaaaat tgatactcca atagaacaga tagatgaaaa aaatatacct
     4381 tatgaaacct ttgggtgtga aaaatatatg cctaaactta atacagaaaa ttcagaagta
     4441 ataaaatatt tattggatgt aggcaagtac tggatacagg aatttgatat agatgcgtgg
     4501 agattagatg tttcaaatga agtagaccat gttttttgga gaaagtttag agaagaagtc
     4561 aaaaaagtaa aaccagacat ttatatattg ggagaaattt ggcatggtag tttaccatgg
     4621 ctaatgggtg accaatttga ttcagttatg aattacttga tgtcagaagc catgaaaaaa
     4681 ttcttttgta cagatgagat aaacgccgaa gaatttaagt atatgataaa tgatgtaatg
     4741 gtaagttatc caatacaagt tagtgaagtt atttttaatt tacttggaag tcacgatact
     4801 acaagaatat tgacttatgc taatggaaat attgatagat ttaaacttgc atatatgttt
     4861 atgtttgtac aaacaggatg tccttgtata tactatggtg atgaaatcgg aatggaagga
     4921 gaattaagtt caatatctga aggtcaaaga aaatgtatgg aatggaatga agaaaaatgg
     4981 aataaggata tatttgattt tatgaagaag attattagac ttcgtaaaga aaataaagaa
     5041 cttaggtcta tatcaaatga atgggtgtta gcagataagg aaaatggaac gattatattg
     5101 aaaaaagaat ctatttctat aataataaat aattcatcta aaaatataat tttgacgtta
     5161 ccagattatt tagcaaataa aaaagtaaaa gatttatatg aagaagaaat agttgattta
     5221 aaagaagaac taatgttaga gaaatacaag ttcattattt taaaataata caaaaagtat
     5281 ttattttaga tttcatgtta aattattata cagtttaaac aagtctttat aaaacataaa
     5341 aaaatatggt ttacaagtga gtgaattgaa agtagatttt aaattaaaga ctgagttaga
     5401 gtagataatt ttataaataa tatcttaaat acaagataga aaataaattg attattagtc
     5461 taaaaatttc ttgtagaaag aggaaataag aatgattgaa gcatttatat ttgatttaga
     5521 tggtgtaatt acagatactg catattatca ctatatggct tggagaaaac tagcccataa
     5581 agttgggata gatatagaca caaaatttaa tgaatcttta aaaggcataa gtagaatgga
     5641 gtcattggac aggatattag aatttggaaa taagaaatat tcattttcag aagaagaaaa
     5701 agtgagaatg gctgaagaaa aaaataatta ctatgtatca ttgatagatg agataacttc
     5761 aaatgatatt ttgccaggca tagaaagctt gttaattgat gtaaaatcta ataatataaa
     5821 gataggtcta tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt tgggtataag
     5881 tgacaaattt gattttatag ctgatgcagg aaaatgtaaa aacaataaac cacatccaga
     5941 gatatttctt atgtcagcga aagggcttaa tgtaaatcca caaaattgta tagggataga
     6001 ggatgcaagt gcaggtatag acgctataaa ctcagctaat atgttttctg ttggtgttgg
     6061 aaattatgaa aacttaaaaa aagcaaattt agttgtagat tcaacaaatc aattaaagtt
     6121 tgaatatata caagaaaagt ataatgagta tatagttagg agaattatat gaaatgttta
     6181 tttagtaatg tagatgagtg gaagattata caagataaga ttgaaatcaa agaaaataga
     6241 ttagcagagt ccataatgag tataggcaat ggatatatgg gtatgagagg aaactatgag
     6301 gaaaattata gtggagatac acacaagggg tcttatatag ctggagtatg gtttccagat
     6361 aaaactcgtg ttggttggtg gaaaaatggg tatccagaat actttggtaa aattcctaat
     6421 tcagtcaact atataggtgt aagaattttc atagatgatg tagaattaga tttagcaaaa
     6481 tgtaaagtag aagattttta tagagaatta gatatgaaaa atggtatttt aaatcgtagg
     6541 tttattgtaa atataaatga taaaaaattt gaagttaggg ttactaggtt tttaagtata
     6601 tctgttaagg aacttgcagt aattaaatat agtataaaat cattaaattc taatactaaa
     6661 ataaaatttg taccatattt agattcaaat ataaaaaatg aagattcaaa ctatgaggaa
     6721 aagttttgga atggaatttc ttcaagttct ttagaatata aaggtagtat tctttctcga
     6781 acaaaggaga atgcatttaa tacaccagta ttcactgtgg gttcaatgat gtatattaag
     6841 acaaatggtg atgtagaagc tgtatctcat gagagtgatt ataattactg tgaaaattcg
     6901 ataacaatgg atgttttaaa aaataaggtt gcaagtatag aaaaatatgt atctataact
     6961 tcaactagag attataagga aagtgagcta ttaagaaaat gtgaagacat tttaaatata
     7021 gaactatcta aaagttatga tgatatatta aatgaacata caaagttatg gaataaaaga
     7081 tgggaaagtg cagatgtaaa aataaatgga gataaaagta gccaacaagg tataagatat
     7141 aatttattcc aactattttc aacatattat ggagaagatt cgcgattaaa tataggtcca
     7201 aaaggattta ctggtgaaaa gtatggagga gctacttatt gggatacaga agcattttgt
     7261 gttccagtat atctaggtgt agcagataag gaggtaacta gaaatctttt agaatatagg
     7321 tacaatcagt tagaacaggc aaaggataat gcaaaaaaac tagggcttaa aggtgcttta
     7381 tatccaatgg taacatttga tggagtagaa tgtcataatg aatgggagat aacatttgag
     7441 gaaatccacc gaaatggaag tatagtatat gcaatatata actatacaaa ttatacaggt
     7501 gactataaat atataaaaga aaaaggaata gatgtaataa tagaaatagc tagattttgg
     7561 gcaagtagag ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg agtaacaggt
     7621 ccaaacgagt atgaaaacaa tatcaataat aattggtata ctaattgtat agctaaatgg
     7681 tgtttagatt atgctgtaga aaatatctta cgattagaga aagagtgtaa tgaatgtatt
     7741 gacagaaata atgtgactag ggaagaagtt gagttttgga aaataatatc aaataaaatg
     7801 tatctgccgt atgatgagga actacaaata ataattcaaa atgataattt tttagataaa
     7861 gaatttgtag aagtaaagga tttaccaaaa gaaaattttc ctcttaatca aaattggtct
     7921 tgggataaga ttcttaggtc gtgttttata aaacaagcag atgtattaca gggaatttac
     7981 tattttggaa atagatttac taaagaagaa aaaaagagaa attttgactt ctatgaaaaa
     8041 tatactgtcc atgaatcttc tttatcttct agtatttatt ctattattgc ctcagaaata
     8101 gataacctag aaaaggctta tgaattatac tcaagaacag ctaggcttga tttagataat
     8161 tacaataatg atacaaatga tggactgcac ataacttcaa tgtctggttc atggctttcc
     8221 attgtacatg gatttgcagg catgagaaca tggaatgaaa ctctatcttt tgaccctaag
     8281 ttgccaaaag agtggaaaaa ttattctttc aatataaatt atagagataa tattattaat
     8341 gtagatgtat gtagtgattt aattaaaata gaaaatttaa aaggtaaacc a
//
LOCUS       DAMKPG010000134         7974 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMKPG010000134 DAMKPG010000000
VERSION     DAMKPG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.140"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            83..544
                     /locus_tag="PC621_003328"
     CDS             83..544
                     /locus_tag="PC621_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4110457.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            612..2858
                     /locus_tag="PC621_003329"
     CDS             612..2858
                     /locus_tag="PC621_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110458.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            2851..4683
                     /locus_tag="PC621_003330"
     CDS             2851..4683
                     /locus_tag="PC621_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110459.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            5402..6679
                     /gene="hflX"
                     /locus_tag="PC621_003331"
     CDS             5402..6679
                     /gene="hflX"
                     /locus_tag="PC621_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF4110460.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            6912..7409
                     /locus_tag="PC621_003332"
     CDS             6912..7409
                     /locus_tag="PC621_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4110461.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            7459..>7974
                     /locus_tag="PC621_003333"
     CDS             7459..>7974
                     /locus_tag="PC621_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF4110462.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIR"
BASE COUNT         3161 a          825 c         1458 g         2530 t
ORIGIN      
        1 ataaatatat tgaaaaaatg catgcagtat agtaaaataa tgcttagccg actaagtaat
       61 ataaaaaata aggagggcaa gtatgaatag caattctgaa gaaaaattaa atgatgtttt
      121 cttgcaatta atgcgaatat attttataag aactcatttt tattttgata aattaggctt
      181 atatcatggt caaccccaaa tacttttttc tttaaagatg aaagatgggc aaagtcaaaa
      241 agaattagca gataaaagga aagtaaaagc atctacaact acagttatga taaagaggat
      301 ggaaaaagct ggacttgtgg aaagaaaaca agatgaaaag gaccaaagag tatctaggat
      361 ttttttgaca gacaaaggtt tagaaatatg taataaagtt gaagaattta acaaggaatt
      421 ggaaagagag ttttttttag gatttagtga agaagaaaaa atagtactta gaagacttct
      481 aatgcaagta cgagataatt taaaagcagg atgtcataag catgaagatg aaaaatattt
      541 ttaaagttta aaataatgtt tttcaattta atgttattag actaattaat attacagaag
      601 ggaagaaatg tatgtttaaa ttaattaaat atttaaagaa atcggcatta tcaatagtaa
      661 taatagtctg tctattggta attcaagcgg tatgtgacct ttctttacca gaatacacat
      721 ccaatatagt aaatgtaggt atacaacaag gtggagtaga aaactctgtt ccatctgtga
      781 ttagagagag tgaattaaat aaaattactt tatttatgga taaaagtagt aaggataaag
      841 tactagataa ttatactttg ctgaataaaa aggattatgt taagtataaa gataagtacc
      901 caggtctaaa agatgaatct ctttatgagt taaatactaa agacaaagac actatagatg
      961 atttaaatgt tatttttggt aaagctattt taatagtatc agggcttgag ggcgatagta
     1021 aagaggtaaa agctatgaaa gctcagttaa tgagtaaact tccaccacaa gctactcaaa
     1081 gtggagatgt tgatatattt aaattgttat cagcaatgcc aaaggaacag ttagatacat
     1141 tgactaaaga gatgagcaaa ggctttgaaa gtatgccaga gagtatgata acacaaagct
     1201 ctgtttcata tgtaaggtca gaatatgaga aaataggtat tgatacagaa aaaactcaaa
     1261 ataattatat attatttact ggtgctaaaa tgcttggtat agcaatgatt agtatggtag
     1321 ctacaataac agtaggattt ttagcagcaa gagtagcagc aagtgtaggt agaaatttaa
     1381 gaagtggagt atttagaaaa gttatgtcat tttcaaatac tgaaatgaat gaattttcta
     1441 cagcttcatt aattactcgt agtacaaatg atattcaaca gatacaaatg ttgatggtta
     1501 tgttgcttag aatagtattc tatgcaccta taatggctat aggtggagtg ataaaggttt
     1561 taaacacaaa tacttcaatg gcctggatta tagcagtagc agtagttgct atcttatcat
     1621 taattgtagt gttattctca gtggttatgc ctaagtttaa actggttcaa aagcttgttg
     1681 ataagttaaa cttagtaagc cgtgaaataa ttactggtat tccagttatc agagctttta
     1741 gtactgaaaa acatgaagaa gaaagatttg ataaagcaaa taaaaattta actaaaacaa
     1801 atctttttgt aaatagagtt atgacatgta tgatgcctgc tatgatgctt ataatgaacc
     1861 ttatcacagt tcttatagtt tggagaggtg catacagtgt tgattctgga gcaatgcaag
     1921 ttggagatat gatggcattt atacaatata caatgcaaat aataatgtca ttcttaatga
     1981 tatcaatggt atctattatg cttccaagag catccgtttc agctacacgt atagatgaag
     2041 tattagtgtc agatataagc ataaaagacc cagaaaaatc agaggcacaa gagtttaagg
     2101 aagataaaaa gggtcttgtt gaatttaaaa atgtttcgtt catgtatcca gacgcagaag
     2161 aacctatatt aacaaatata tcttttacag caaagccagg agaaacaact gcctttatag
     2221 gaagtactgg tagtggtaag tcaactctta taaatttaat tcctcgtttc tttgatgtta
     2281 ctgaaggaga aatattactt ggtggagtta atataaaaaa tgtttctcaa catgatttaa
     2341 gggaaaaaat tggatatgta cctcaaaagg gagtattgtt ttctggtact atagaatcca
     2401 acttaaaata tggtagaaaa gacgcaacag atgaagaaat gagaacagca gctgaaatag
     2461 ctcaagctac agaatttata gattctaaac cagaagcttt taaaactgaa atttctcaag
     2521 gtggaacaaa tgtatctgga ggtcaaaaac aaagactttc tattgcaaga gcaatagcaa
     2581 aaaatccaga agtttatata tttgatgata gtttttcagc acttgactta aaaactgatg
     2641 cagctcttcg taaggcttta aaatcaaaaa ctgctgaaag tacagttcta atagttgcac
     2701 aaagaattag tactatctta aatgcagaac agataattgt tttaaatgag ggagaaatag
     2761 ttggaatagg aacacataaa gagcttctaa aaaattgcga agtttatgag caaatagctt
     2821 tatcacaact ttcaaaggag gagctagcta atgagtagaa tgggaagagg tcctatggga
     2881 aaatctatgg aagcaggaca aaaagcaaat gactttaaag ggactatgcg aaaactaatt
     2941 gcatatttat ctaaatttaa aatatcaata attttagtaa ttgtatttgc tattggtagt
     3001 gcatcgtttt ctatagtagg tccaaaaatt ttaggaaagg caactacaaa gatatttgaa
     3061 gggttagtga gtaaagtttc tggaggaaac gtaggtatag attttaatgc aattggaaaa
     3121 atcttaacct ttttactttt cctttatttg ataagtgcac tgttctcttt tatacaagga
     3181 tttataatga gtggaatttc tcaaaaagta tcctataatt taagaaaaga gatttcagca
     3241 aaattggata gattaccaat gaagtatttt gatactaaga cgcatggtga aatattatcg
     3301 agaattacga atgatataga tactttaaat caaagtttaa atcaaagtat gacacagctt
     3361 ataacatcag taacaactat gataggtgtt ttgataatga tgttatctat aagtggaatt
     3421 atgacattag tggcagtttt aatattacca atatcaatgt ttgtaatttc aagaataata
     3481 aagaaatcac aaaaatactt tagatatcaa caagaatatt taggaaatgt aaatggtcaa
     3541 gttgaggaaa cttatagtgg ccaaacaata gtgaaagcat ttaatcgtga agatgaagta
     3601 attgaagagt ttgataaact aaatgatagc ctgtataatt cagcgtggaa atctcaattt
     3661 ttatcaggta taatgcaacc acttatgatg tttattggaa acttgggata tgtaatggta
     3721 tcaatcttag gaggttggct tgcaattaag aaaacaattg aggttggtga catacagtca
     3781 tttatacaat atgtaagaaa ctttacacag ccaatgactc agattgcaca ggttgccaat
     3841 ttattgcaat caacagcagc agcttctgaa agagtatttg agtttttaga agaggaagaa
     3901 gaagtccaaa ttgttgaaaa tgcagtttca atagatggtt tagaaggtaa aattgatttt
     3961 gaaaatgtca actttggata taacccaaac aagactataa taaatgattt ctcagtaaat
     4021 gttaaacctg gtcaaaaagt tgctattgtt ggacctacag gagcaggtaa aactacaata
     4081 gttaaactgt taatgagatt ttatgatgtt aatagcggtt caatactgat tgatggtcat
     4141 aatattaaag attttaatcg tagtgagctt agagaaatgt ttggtatggt tcttcaagat
     4201 acatggttat ttagtggttc aattatggaa aatataagat atggtaagtt gaatgctaca
     4261 gatgaagaag ttatagaggc tgctaaatct gcacatgttc atagatttat taagacgtta
     4321 ccagatggat ataagatgaa attaaatgaa gaagcaagta atgtttctca aggtcaaaaa
     4381 caattattga ctatagctcg tgctatttta gctgatccta aaattttaat attagatgaa
     4441 gctacaagtt ctgtcgatac tagaacagaa gtattgattc aaaaagctat ggataatctt
     4501 atggaaggta gaacaagttt tgttatagcc cacagattat caactatacg tgatgctgat
     4561 atgattcttg taatgaatga aggagatata gttgaacaag gaaatcatga ggaacttcta
     4621 aagaaaggcg gattctatgc caatctttac aattctcaat ttgaagaaga tgaagcaatg
     4681 taacaaaaaa ctatcttagc atagacatgt aattaataag ataaaataaa tgagggtgcc
     4741 ttaaaatgaa ctttttaatt cataaggcac tcttttgtgt atgaaataaa gtgtattttt
     4801 atcattttaa caatgaaaaa gaggattatc tatattttga gatagccaca taattttatg
     4861 tctttacaaa gtgtttattt tattataata tgtagtaagg aaaatgttgg aggtgattat
     4921 caaaatgtat atttaatttt attctttaaa ttttattata aatagaattt aattttaata
     4981 gttgatgatt atattgaagt atatgttttt attactttta ttaatcatgc aaataactac
     5041 ttaaatttaa gaatagggtt aaatataaaa tgataattac atataagtta attaaactaa
     5101 tgtattttta atatatattt agaattttaa tccttcttat gatataagta gcttgttttg
     5161 tattacttag atttgtaagg agttttttta ttgaaaaaac tgcataatat atttgggagg
     5221 aggtgtaaga aagtgatttt ttatataaga ttgagaaaat aatgcacgat tatggttatt
     5281 ataatagtaa ataagaagtg tcatttaagg ataagataaa aagcaaaatt cttgatgaat
     5341 ttaattatag agccataatc tcgtgctata gattaaataa aaaatagcag ataaggagaa
     5401 tatggatatg acgaaaaaag gaataacagt aggattaaac ataaataata aatctgagga
     5461 ctttaatgaa ctaatgattg aacttgaaaa cttatgttca gcatgtgata ttgatgttgt
     5521 agggagtata actcaaaatg caaagcaggt taatagagca ttttacattg ggacaggtaa
     5581 agttgaggaa atattaaact taataaagaa agaaaacata gagatagtta tattttacaa
     5641 tgaactttct acatcacaac taaaaaatct tgaagaaaag cttaattgtg aaattataga
     5701 tagaacggca ttgatacttg atatatttgc acaaagggca aaaactagag aagctaaact
     5761 tcaagtagaa gtagcaagtt taaaatatat gttaccaaga cttataggtt caaatgaaaa
     5821 tttaggacgt caaagtggtg gagttggtac taaaaataga ggttctggtg agaaaaaatt
     5881 agaacttgac cgtagaagaa tagaagaaaa gataacatct ttaaataggg agttgtatga
     5941 tttaaaattc caaagagaaa ctcaaagaag tatgagaaga aaatcaaatc ttccaaatgt
     6001 agctctagtc gggtatacaa atgctggtaa atcttcaata atgaataaac tagtagatat
     6061 atttaaaaac tctgaagaaa agaaagtgtt tgagaaaaac atgttatttg ctacacttga
     6121 aacttctgtc agaaatatag ttcttgcaaa taacaaagaa tttttattat ctgatactgt
     6181 tggttttgta agtaatctac cacatgactt agtaaaagca tttaggtcaa cattagaaga
     6241 agcatgtgag gcagacgtac tgttacatgt tattgatatt tcaaatccaa gctataaaag
     6301 tcatattaaa gtgacagaag atactttaaa acaaataggt gcagatggta tacctatgat
     6361 acatgtgtat aataaaattg atttaataga tgtagaagta ttggatagaa ttttagatag
     6421 tatcgataaa gaaggtatat ttgtttcagt taaaaaagat ataaatatag ataaaatgat
     6481 aaaatgtatt tgtgatagca tatttaaaga ttgtgtaaga tgtaagttct taattccata
     6541 tgataaaggt catgtagttt cttattttaa cgaaaatacg agcattataa atacagaata
     6601 cagagaagat ggagctattt tagatgtaga atgtagcaat atagaatata ataagtataa
     6661 aaagtatgct ttagagtaaa ttttgaaaaa cttattatag cctaaaatat atttaattta
     6721 taatttatta taaattgatt gtaaatatga ttttatgaaa cggtatagcc ataatgctat
     6781 gccgttttgc catttataat aaaaataaat ttattgacac aatagtaact aattactata
     6841 atataaagta actagttact atttaaaatt atcaaaaaat taaaatctaa gataaagaga
     6901 agtgtataat aatggatgca gaaagtttaa tgaaaaaata tatggtagat gatgatagta
     6961 gaaaacaagc tatatttgca agtatatttg taatgcaaaa taaacttcaa actacatgtg
     7021 ataaattaga cccagaatta acaatgaaac aatggttact tttagctatt gcggactctg
     7081 tagatgaaac tcttacatta accaaacttg gagaattaat ggggtgctca agacaaaatg
     7141 taaaaaaact agctattgct ttagaaaaga aaggttttat acaaattgag caaaatgaaa
     7201 aagattcaag agctatctgt cttgtgacaa aagatagagt gcaagagtat tctaaaaaag
     7261 taagaggtat gcaggaaaaa gtattacagc ttttatttga agattttaca gagaaagaag
     7321 tggaacagtt attttttgga attatgaaat taagtatagg aattgaaaat gtgaaaaatt
     7381 atgtagaaaa taatgacgtt ggagaataag caagtatcaa atcaactttg tatgacatca
     7441 gagagggggg taatttatat gaaaagtgta gtagtgattt ataagagtaa gtatggttca
     7501 acaaaaaaat atgctaaatg gctttcagaa ttattgtctt gtgatatttt tgaaactaag
     7561 aatattaata tagaaaaggt tatgaagtat gatacgatta ttttaggagg aggtctttat
     7621 gcttgtaata tagcgggaat ttctttctta aaaaaaaatt ataaagattt gcagaaaaaa
     7681 aatttagtag tatttggcgt aggagctgct ccatatgatg atgaagtaat aaagaaatta
     7741 agagaagtaa actttaagga tgaattaaac aaagttcctt gcttttattg tcgtggggca
     7801 tgggatgaga caaaaatgtc ttttatagat tatactcttt gtagtttttt taaaaaatct
     7861 atagctaaaa aagaacgtca aggtcttaaa ccagcagaag cacctttatt gtctggagaa
     7921 gcctttgatt ggtgtaataa agaaaattta actccaatac ttaattatat aaga
//
LOCUS       DAMKPG010000135         7939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMKPG010000135 DAMKPG010000000
VERSION     DAMKPG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.141"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            27..101
                     /locus_tag="PC621_003334"
     tRNA            27..101
                     /locus_tag="PC621_003334"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC621_003335"
     tRNA            116..200
                     /locus_tag="PC621_003335"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC621_003336"
     tRNA            210..293
                     /locus_tag="PC621_003336"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC621_003337"
     tRNA            317..391
                     /locus_tag="PC621_003337"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC621_003338"
     tRNA            416..492
                     /locus_tag="PC621_003338"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC621_003339"
     tRNA            502..577
                     /locus_tag="PC621_003339"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC621_003340"
     tRNA            586..661
                     /locus_tag="PC621_003340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC621_003341"
     tRNA            664..752
                     /locus_tag="PC621_003341"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC621_003342"
     tRNA            756..831
                     /locus_tag="PC621_003342"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC621_003343"
     tRNA            838..911
                     /locus_tag="PC621_003343"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC621_003344"
     tRNA            926..1002
                     /locus_tag="PC621_003344"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC621_003345"
     tRNA            1020..1096
                     /locus_tag="PC621_003345"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC621_003346"
     tRNA            1105..1180
                     /locus_tag="PC621_003346"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC621_003347"
     tRNA            1188..1261
                     /locus_tag="PC621_003347"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC621_003348"
     tRNA            1269..1345
                     /locus_tag="PC621_003348"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC621_003349"
     tRNA            1358..1433
                     /locus_tag="PC621_003349"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC621_003350"
     CDS             1509..1922
                     /locus_tag="PC621_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110463.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC621_003351"
     CDS             2231..5662
                     /locus_tag="PC621_003351"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF4110464.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC621_003352"
     CDS             5904..7832
                     /locus_tag="PC621_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF4110465.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3102 a         1018 c         1596 g         2223 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagaacttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccacta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcgct
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cagtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaag attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gccgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7381 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcgcaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttatac
//
LOCUS       DAMKPG010000136         7756 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMKPG010000136 DAMKPG010000000
VERSION     DAMKPG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.142"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            61..1119
                     /locus_tag="PC621_003353"
     CDS             61..1119
                     /locus_tag="PC621_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF4110466.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCIFLENCDFNRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1223..2407
                     /locus_tag="PC621_003354"
     CDS             1223..2407
                     /locus_tag="PC621_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF4110467.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2614..3327
                     /gene="rpsB"
                     /locus_tag="PC621_003355"
     CDS             2614..3327
                     /gene="rpsB"
                     /locus_tag="PC621_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF4110468.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3406..4317
                     /locus_tag="PC621_003356"
     CDS             3406..4317
                     /locus_tag="PC621_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF4110469.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4402..5106
                     /locus_tag="PC621_003357"
     CDS             4402..5106
                     /locus_tag="PC621_003357"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF4110470.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5132..5689
                     /gene="frr"
                     /locus_tag="PC621_003358"
     CDS             5132..5689
                     /gene="frr"
                     /locus_tag="PC621_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF4110471.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5692..5895
                     /locus_tag="PC621_003359"
     CDS             5692..5895
                     /locus_tag="PC621_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110472.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5924..6658
                     /locus_tag="PC621_003360"
     CDS             5924..6658
                     /locus_tag="PC621_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF4110473.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6671..7450
                     /locus_tag="PC621_003361"
     CDS             6671..7450
                     /locus_tag="PC621_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF4110474.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3190 a          792 c         1465 g         2309 t
ORIGIN      
        1 aataatatcc aaactaatac aaaatagtaa taattgtttt gtactggagg atatatattt
       61 atgaattgta aatggatatc taagatagat gagagaaaaa aatgtcatag agaagcagat
      121 tcttctggat attgtatctt tcataaagaa aataagtctg atgaagagat acagttgatg
      181 atggatacta ttcataaaga agagataagt gaatttaatg gatttgtttt tgaaaatgaa
      241 tttaatgcgg aagaaatatt aacttacaat tataaaatac tggatttttc tgaatccata
      301 tttaaacaaa aagcaaattt taaaaaatat atttttaaaa agaatattat ttttaactat
      361 acagaattta gagataaagt tttatttaat ggttgtatat tcttagaaaa ctgtgatttt
      421 aatagaacca tatttagtaa aaactatata aatgaccgta tatttgaaaa agtgaaattt
      481 aaaggacctg atttggtagt taataaagta gaaaattttc cacgaatgga tggaataatt
      541 tttagcatgt gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag tgagtatgag
      601 catggcaaga taaattatag aatagctaga aatcaagcaa caaaaattgg agaatatgag
      661 atgataggat tttactatta taaggaaaga atttatagta gtaaaataat gaaacgttct
      721 aattatccta ctttcagtga ttatttagtt gaaaaatttt ttgaccaaat agcacgttac
      781 acaacaggtt atggagaaaa gccatggaat atattattag tgattatagc aataataagt
      841 gtttttgctt tattatactt atttgtaggc atagaaagtt caaatagcac acttgtagct
      901 ttagatataa acaatatagg tgattactca ctaagtgaga ttttcaaaat gtatatggat
      961 ttatggtatt ttagcatggc tacatttagt acagtaggat atggagatat ggttgctaca
     1021 agtttgattg ggaaagcatt ggcaggtata gaagtatttt ttggagttac aataggagca
     1081 atttgggcat ctgtgattat taaaagaatg ataaggtaat atttgtgtaa atatggatag
     1141 gtattaatta ctagatataa attggaccaa atagggaaaa tttaaatata aatatactta
     1201 ataatccaag aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat cattagccat
     1261 gattctagtt tttataatac caaattattc atatgcagat ggtaaatctg tagacaagta
     1321 ttcaaaaaca tctatactta tagaccaaga aacaggtaga gttttatata gtaaagaacc
     1381 tgatatgaag gtaccattag ctagtttaag taagatgatg acttttttac ttgcaataga
     1441 agctattgaa aataaagaag taaaagaaac tgataaggta aaaatagaca aatctattgc
     1501 atcagtaaaa gggtcaagtt ataaacttaa agatggtgaa gaagtgccac ttatagagct
     1561 tatgagaggt cttatgattg tatcaggaaa tgatgcggca attgctatag caaaacatat
     1621 ttgtaaaacg aaagaagcat ttgtagatag aatgaataaa aaagcaaaag aaataggaat
     1681 gagtaataca cattttctaa atccaaatgg tcttccaata tacgatttaa gcaaccccaa
     1741 aaaaccagca aaagaaaata tctctactgc aaaagatata gctattcttg gaaaatacat
     1801 gtttgaccat tatgaaaaac aagtaacagc tataactgat atggagacat atacaaattc
     1861 agatagaagt tttgaaaaaa gcaatacaaa tgcattatta agaattatac cagaagtaga
     1921 tggtataaaa actggatata ctggcaatgc tggatattgc ttatcatttt ctatggttgt
     1981 aaataaaaat gagaaaaatg agaaaaaacg tagagtaata ggggttgtat taggtactaa
     2041 ccataaaaat aaaagaacct ctgcttcatt agctctactg aaatatggaa aagaaaattt
     2101 caatatgaaa aaagtaattg ataaagatag ctttattggt aagaaatata taaaaggaat
     2161 gaaagagtta gaggttacat taaaagcaaa agatgaactt tatacaatat taaaagatga
     2221 tgaaagtata aaatcagaag ttaagattaa aaatatagag taccctgtta aaaaaggtga
     2281 tactatgggt gttataaaat attatactag ttcaggtgac ttacttggaa gtgtagatat
     2341 agtaagtaat aatagtatag ataacgtatc attaaaaaca aaattaaaaa tgaaatttgc
     2401 aaattaaatt caaaaaatgg tataaaatac acaaaaaaat ataaaaatag aaaatatgga
     2461 aaaaatattg ttatttcttc gcctctgtga tatactacta gaggtgaaaa aataaaataa
     2521 atcacacaca gcattgggat ccttaaaagg tgcctattac atggtttttt agggagaaga
     2581 tcaagctgga ggtataaaac caggaggtaa gatatgtcag taatatcaat gaaacaatta
     2641 ttagaagcag gtgttcactt tggtcaccaa acaagaagat ggaaccctaa aatggctaag
     2701 tatatattca cagagagaaa tggaatatat ataatcgact tacaaaaaac agttaaaaaa
     2761 gttgaagaag cttacaagtt cactaaagaa gtagctgaaa caggaaagcc aatcttattc
     2821 gtaggaacta aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag atgtggaatg
     2881 tactttgtta atgaaagatg gttaggtgga atgttaacaa accacaaaac tataaaaaca
     2941 agaataaata aattaagaga attagaaaaa atggaagaag aaggcgtatt taatgttctt
     3001 cctaaaaaag aagttataaa attaagagct gaaaaagaaa aactagaaaa atacttaggt
     3061 ggaataaaag acatgcctga attaccaggt gcaatgttcg tagttgaccc aagaaaagaa
     3121 aacatagcta ttcaagaagc tcatagatta ggtataccag tagttggtat agttgatact
     3181 aactgtgacc cagaacaatt agactttgct ataccaggaa atgatgacgc tataagagcc
     3241 gtaaaattaa taactggtgc tatggcaaca gctgtaattg aaggtagaca aggtgctgag
     3301 gaagaagtag ctgaagacca agaataataa tttgacaatt aaatggtaag ggtcgccctt
     3361 accatttata atatggaata gaataagtag gaggaaactt aaaaaatggc taatataact
     3421 gctcaaatgg taaaagagtt aagagaaagt acaggcgcag gaatgatgga ctgcaagaaa
     3481 gctttacaag aagctgaagg aaacatggaa aaagcagtag atttattaag agaaaaagga
     3541 ttatcaaaag ctgctaaaaa agctggtaga gttgctgctg aaggtctagt tgcaatagaa
     3601 atgaatgatg ataatacagt tgcttctatg gtagaagtta actcagagac agatttcgtt
     3661 gctaaaaacg aagacttcaa agtgttcgtt aaagatgctg cttgtatggc attagctact
     3721 gataaagaag atatagcttc tttattaggt gaaactcata aagagggaat aactttacaa
     3781 gaagttttaa ataatagagt tgcaaaaata ggtgagaaat tagactttag aagatttgct
     3841 aaagttgtaa ctaatggaca agttgctgga tacattcacg gtggtggaaa aataggtgtt
     3901 cttgttgaaa tggaaacaga agctagagat gctaaagttt tagaattagg taaagatgta
     3961 gctatgcaag ttgctgctat gaatcctaag tatgtttcaa gagatgaagt agatgctgaa
     4021 tacatagcac atgaaacaga agtattaact caacaagctt taaatgaagg aaaaccagct
     4081 aatatagttg aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga ggtttgttta
     4141 ttagagcaaa ctttcgttaa aaatccagat ataactgtta aacaattagt tgctgatgtt
     4201 gctaaggcag taggttctga tataaaagtt gttaaagttg tgagattcga agttggtgaa
     4261 ggtatacaaa agagagaaga aaactttgca gaagaagttg ctaagcaact taagtaataa
     4321 ccaaagagag aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt ggatatgaag
     4381 gattgaaata ggaggtttct gatggataaa ccaatgtaca aaagggtatt gttaaaatta
     4441 agtggagaag cattagcagg agaaagaggt tttggtataa ataatgatgt agttaatgat
     4501 attgctattg ctataaaaaa aatacaagaa ataggtgttg aagtagctgt agtagtaggt
     4561 ggaggaaact tctggagagg cagaaccagt gaaggaatgg atagaactac tgctgactat
     4621 ataggaatgt tagctacagt tatgaatgca atggcgctac aggatgcact agaaaatata
     4681 gatgttgcta caagagttca aactgctata gacatgagac aaattgcaga accatatata
     4741 agaagaagag cagttagaca cttagaaaag gaaagagttg ttatatttgg tgcaggaaca
     4801 ggaaaccctt actttacaac agatacaact gctgctttac gtgctgctga gatggaagca
     4861 gaagttatat tgctagctaa aaatgttgat gcagtatatg acaaggatcc aaaagtacat
     4921 gcagatgcta agaaatttac agaattaagt tacatggaag ttatacagaa agaattaaaa
     4981 gtaatggatt ctacagctac atctttatgt atggataata aaattccaat aaaggtattt
     5041 gagcttacaa cagagaatat aataagagca gtaaaaggcg aaaatatagg aactactgta
     5101 aaataatgaa gaatgaagga ggagtaaaat tatgaaactg gaaatacata agcaattaga
     5161 agagaaaatg aatgggacaa ttgatgcttt aaagtttgaa tttggaacta taagagcagg
     5221 aagagctaat gcacaaatgc tagacaaaat aagagttgat tattatggaa ctccaactcc
     5281 aataaatcaa ataggtgcta tatctgtacc agaaccaaga atattaatga tatctccttg
     5341 ggataaatct gctatgcatg aaatagaaaa agcgatagct aactcagact taggattaaa
     5401 tccatcaaac gatggtgagg ttataagact ttctgtacct gctttaacag aagaaagaag
     5461 aaaagaatta gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta gaataagaaa
     5521 tgaaagaaga gacgccaatg agaaaattaa aaagatggaa aaaggcggag aattaactga
     5581 agatgaatta aaaaaagcac aagatgaagt tcaaaaaatg actgataaat ttataaaaga
     5641 aatagataca ttattatcta aaaaagaaaa ggacataatg gaggtataat attgaaaaaa
     5701 tattataacc ttttaggtct tcatgtagat gaggtaaaac aatattttga tgagcaaaat
     5761 ataaattata ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact tgtagttcct
     5821 agagttataa aaatatctga gataggtgat agtgtagaac tgataatcac atacttttct
     5881 gactccttag tttaaggagt cagaacaata ttggaggcaa aatatgaata acaacattat
     5941 atatgatata gacttaaata acatacctac tcatattgct attataatgg atggaaatgg
     6001 aagatgggca aaagctagat ttcttccaag aactgcagga cataaggctg gagtagaaac
     6061 tataagagat atagtaaaag aatgctcaaa attaggtgtt aagcacttaa cattatatgc
     6121 attttcaaca gaaaactgga aaagaccgaa acttgaagtt gacacactta tgaacttatt
     6181 atctacatac ttaagaaatg aaatagctga acttcaccaa aacaatgtaa aagtaactgc
     6241 tataggagat ataagtgctt taccaaaaac ttgtataaga gaattaaact cagctaagga
     6301 actgacaaaa aacaatacag gagttaactt aaatttagca ttaaactacg gaagtagagc
     6361 agatataaaa aatgctctta tagatatagt aaaaaattgt gaaagtggta aaatagatat
     6421 aaacaatatt gatgaagata ttataaaaaa ctatttaagc acaaagtcta taccagatcc
     6481 agacttggta ataagaacaa gtggtgaaca aagattgagt aactttttac tttgggaagt
     6541 tgcttattct gagttttact ttactgatat acattggcct gattttaaaa aagaagaact
     6601 acagaaggct atatacgtat atcaaagaag ggatagaaga tttggaggac ttaagtaagg
     6661 agattatttt atgcttacaa gaattattgc ttcactagct ctggttccat tatttctatt
     6721 tgttgtatat ggaggtattc ctctatatat agctgagaca gcaatagttt atattgcatt
     6781 gcatgagttt tataaggcat ttaaaataaa agatgtacat ccaatattca ttataggata
     6841 tttattttct atttatttag ctgtaaaaaa tatttttaat ttaccattag aatatactta
     6901 tgcagtaatt tttattttat ttttagcaag tataatttat atgttgatgg gcaaaaacaa
     6961 tgttatagat gtttcgatta cgtttttagg agttttttat ataggagtat ttttagattt
     7021 tataattata actataaatg gatttgaaaa aggtagtata tatgtatggc ttatatttgt
     7081 gatatctttt atgacagata tatttgcata ttttagtggt tatttacttg gaaaacataa
     7141 gctaattcct aaagttagtc ctaaaaagac tatagaagga gctattggag gaataatagg
     7201 aagtacactt tgttgtattt tatttggata tttatttggc attgatttgc ttcaattagc
     7261 gataatagga agtataggaa gtgtaatagc tcaattagga gatttatttg catcttctat
     7321 aaagagatat gtagggataa aggattatgg aaagataata cctggtcatg ggggtatatt
     7381 agatagattt gatagtgtta tattagttgc accttttgta tatagtgcga ttaaattttt
     7441 tattagataa agattggttg gatttaaaag agcaatctat aattgctagt aatgaaaata
     7501 gctatgtaac ttttaaatgc agagatattc tgcagttatt agttacatgg ctattttcat
     7561 tgttcaaaaa tttagaagac cttggggaat tttataagaa taagtattac tttataatag
     7621 tatactatat ttgtagtgag tatatttttt atactataac tatcattaaa aaatattttt
     7681 ttgtttaata atttcgatat ttttagcttt attatatatt tataatatat atgtataaat
     7741 gtaactaatt tattat
//
LOCUS       DAMKPG010000137         7479 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMKPG010000137 DAMKPG010000000
VERSION     DAMKPG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.143"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            99..1076
                     /locus_tag="PC621_003362"
     CDS             99..1076
                     /locus_tag="PC621_003362"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF4110475.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1069..2037
                     /locus_tag="PC621_003363"
     CDS             1069..2037
                     /locus_tag="PC621_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF4110476.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2221..2811
                     /locus_tag="PC621_003364"
     CDS             2221..2811
                     /locus_tag="PC621_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF4110477.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2830..3600
                     /locus_tag="PC621_003365"
     CDS             2830..3600
                     /locus_tag="PC621_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF4110478.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3737..4705
                     /gene="dusB"
                     /locus_tag="PC621_003366"
     CDS             3737..4705
                     /gene="dusB"
                     /locus_tag="PC621_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF4110479.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4814..5293
                     /gene="greA"
                     /locus_tag="PC621_003367"
     CDS             4814..5293
                     /gene="greA"
                     /locus_tag="PC621_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF4110480.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5308..6837
                     /gene="lysS"
                     /locus_tag="PC621_003368"
     CDS             5308..6837
                     /gene="lysS"
                     /locus_tag="PC621_003368"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF4110481.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7146..7340
                     /locus_tag="PC621_003369"
     CDS             7146..7340
                     /locus_tag="PC621_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110482.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         2985 a          753 c         1511 g         2230 t
ORIGIN      
        1 gaaatttaaa attaatagta ttaaaagtat attaataatg tataattatt tattagaaga
       61 aaataagtca tgaaattatg tcatattaga ggtgaaatat gcgagataaa ataatagaga
      121 ttatattaaa taataaatct gaatttattt caggagaaga attatcaaaa caattagggg
      181 tgtcacgtgc agctatatgg aaacatatga aagccttaaa agaagaggga tataatatag
      241 aatctgtaaa taaaaaaggg tatcgattag cggaaaatcc aactgattta ttaagcccac
      301 agaatattta ctataaatta gatacagagt ttattggaaa aaatattgaa catcttgaca
      361 ctatagattc aacaaatgaa catgctaaaa ggatagctat aaatagtgta gatgggactg
      421 tgattttaag tgaacatcaa gatatgggga aaggacgatt gggaagaaga tgggattcaa
      481 aaactcatga aggaatctgg atgagtatta tattaaaacc agatatggaa ccattcaaag
      541 ctccatttat aactttaata gcaggagcaa gtgtaacgaa agctttaaac aatctaggag
      601 caaatacatt aataaaatgg ccaaatgatg ttattttaaa tggaaaaaaa atatgtggta
      661 tattaacaga gttatctgct gaaattgaaa gaacaaatta tgttgttctt ggaattggaa
      721 taaatgttaa aataatggat tttgaacaag aaatagctga caaagccaca tcattgtata
      781 aagaagggta tatgttatcc agagtagata ttgttaggaa tgtattgact gaatttgaaa
      841 aattatattt agattatact gtaaataatt ccaaagaaaa aaccttaaaa atatgtagag
      901 attattctgc aattatagat aaaaatatat atgtattaaa aggagataat agagaactag
      961 tgaaatgttt agacataaat gaagagggaa atttattagt tcaaagaagt gatggaagta
     1021 taaaagaaat tatttcaggt gaagtttcta taagaggaga aaaaggatat gtataataat
     1081 attttggata tagaaggctt aaaggttggg caggttgagg atagaaatgg tcttactggg
     1141 tgcacagtag taatatgtga agaaggagct acttgcggtg ttgatgtaag gggttctgct
     1201 ccagggacta gagaaaccga tttattagac cctgtaaata tggttcaaaa agttcatgca
     1261 gtagttttgg ctggaggttc tgcatatggt cttgaatcca cttgtggtgt aagtagatac
     1321 ttagaagaaa aaggaattgg ctttgatgta ggtgtaggta aagtgcctat agttgtagga
     1381 gcagttttat ttgatttagg agtagggagc tttaaatgta gaccagattt aaagatgggt
     1441 tatagagctt gtgaagttgc aaatactgat attttaaatc aaggaaatta tggagcaggt
     1501 tgtggagcca ctgttggaaa aataagaggt caggaatatg ctatgaaagg cggaataggt
     1561 agctattcta taaaattaga taatggtcta attgtatctg cattaattgc tgtaaatgct
     1621 cttggagatg tttatgaaaa tggtaaggtt atagctggtg ctttaaatga taaaaagaca
     1681 gaaacaattg atacatataa attaatgaaa caaggtgaaa gtaaaggtgg atttagtgta
     1741 gataatacta caattgggat aattgcaaca aatgctaagt taaccaaagc tggatgtaaa
     1801 aagatatctc aaatgggaca tgatggatat gctaagtcta tttttcctat ccatacacct
     1861 catgatggag atacaatatt cacaatatca acaggagaaa tagaagcaga tataacatta
     1921 gtaggttcat tggctgtaga ggttgtggaa aaaagtataa taaatgcagt aaaaaaagcg
     1981 gatagtgtag aaaatataat ggcatataaa gatatagtaa aaaaacgaga aatatgatga
     2041 aattagcata tttttcttgt caaattgttg acaatggcaa ctcatcttga taaaattatt
     2101 cttgttagag cgggcatcct acaatggtag cttgactcat agttataaga aaaaaacgaa
     2161 aaaaattaaa aaattaagta gtccggcatc ttaatgagct ggaacggagg tgtatttttt
     2221 atgatgaaca caagtgcatc agtaagaaaa gtaaatgtaa gaaagatgac tataatagga
     2281 gtactatcag ctatttctat tatgctatct atgactccat tgggatttat tccagttgga
     2341 cctacaaaag caactataat gcacatacca gttattattg gagctattat ggaaggacca
     2401 atagttgggg cagcaatagg atttatattt gggatatcaa gtctcttaaa tgctataaca
     2461 aacccaacag taacatcttt tgtttttata aatccattag tttcaatatt accaagagtt
     2521 atgataggtg tattagctta ttatgtatat cagttaataa ttaaggctac aaataaggta
     2581 tacatatcag gtttaattac aggggcaata ggttctctgt taaatacagc tggagtttta
     2641 ggaatgatat atgtcttata tgctgacaag tatcttcatg caatgggtaa aagtggttca
     2701 gcaggaaaag taataatggc tcttgccgca gcaaatgggg tgccagaagc aatagtaggg
     2761 gctttagtag tagctgctgt tgccactgta cttaagaaaa gcaaaaaata gaaagaaggt
     2821 taatgacata tgcttctagt atttgatgtt ggaaatacta atatggtttt aggtatatat
     2881 aaaggtgaca aattagttaa ttactggaga attaaaacag atagggaaaa aacgtctgat
     2941 gaatatggaa tcctgataag taacctattt gattatgata atgtgaatat aagtgatatt
     3001 gatgatgtta taatatcatc tgtagttcca aatgtcatgc attctcttga aaacttttgt
     3061 ataaagtact gtaaaaaaca gccattaata gtaggtccag gcataaaaac aggtctaaat
     3121 ataaaatatg ataatccaaa acaagttggg gcagatagaa tagttaatgc tgtagcaggg
     3181 atagaaaagt atggagcacc aagtatactt gttgattttg gaacagcaac tacattttgt
     3241 gctatctctg aaaaaggtga atatttgggt ggaacaatag caccaggaat aaaaatatct
     3301 agtgaggcgt tatttcaaag tgcgtctaaa ttacctagag tagaattagc taagccaggt
     3361 atgactattt gtaagagtac tgtatcagcc atgcaatctg gaataattta tggatatgtt
     3421 ggtttagttg acaaaataat aagtatgatg aagaaagaat tgaattgtga tgatgttaag
     3481 gttatagcta caggtggatt agctaaactg attgcttcag agacgaaaag tatagattat
     3541 gtagatggtt ttttaacact agaaggattg agaataatat atgaaaaaaa ccaagaataa
     3601 ttaatataca gggaattggg ttatatatcc aattcccttt ttatgataga tttctagcta
     3661 tatgtggtat attataatga ggaaataaga atttaaagtt aaatcacaaa ataaaggcaa
     3721 aaataggaga aaaacaatga aaataggaaa tttagaattg aaaaataaag tttttctatc
     3781 acctatggca ggtgtaacag accttccatt tagattaatt tgtaaagaac agggatgtgg
     3841 gcttttatat acagaaatga taaatggtaa ggcactttgc tatgatgatg aaaatactaa
     3901 aaaaatgctt aagatagaag aggaagaaca tcctgtagca gttcagatat tcggttcaga
     3961 accagagttt atgggaagag cagcagagat aatgaatgat tattcaaatg agattctcga
     4021 tataaatatg gggtgtccag ctccaaaagt agttaaaaat ggagatggtt cagcacttat
     4081 gaaaaatcct aagcttgcag aagaggtatt gagggcagta gtgaagaact caaaaaagcc
     4141 agtgacttta aagattcgaa aaggctggga tgataatagt gtaaatgctg ttgaaatagc
     4201 aaagatagca gaggattgtg gaataagcgc tttagcaata catggaagaa ctagagaaca
     4261 gttttataca ggcaaagcag actgggatat aattgcagag attaagaaaa atttgagtat
     4321 acctgttata ggaaatggtg atgtatttac aatagaagat tcaataaata tgttagacaa
     4381 aactggttgt gatgctataa tgataggtag gggtgctcaa ggtaaccctt ggatatttaa
     4441 aagaataaac cattatatga atacaggcga gattttgcca gagccaactc ttaacgaaaa
     4501 aataagtact gccattaagc atttaaaact tgcagtagaa gaacatggag agtatgtagc
     4561 tgtaagggaa atgagaaaac acatcgcttg gtatttaaaa ggacttagaa actctgcaaa
     4621 attaagggat gaaattaaca aaatagaaga ctatcaagaa gttgtgtcga aattggagta
     4681 ctatatggaa gactccttga cataaaaata atagtgactt ataatatagc gcataaatta
     4741 aaaaaagatt ataaaatttt taaaatataa ttaggaaaat tcctaagaaa tttgtgaagg
     4801 agttgtattt ataatggaag aaaataaaga gtttttattg acacaagagg gatatgataa
     4861 attagaagaa gaattagaaa acttaaaggt tgtaaagaga aaagaagttg cagagagaat
     4921 aaaagttgct atatcttttg gtgatttatc tgaaaatgct gagtatgatg aagctaaaaa
     4981 ggaacaagca caagttgaag aaagaatttt aaaattagaa aatatggtta gaaaagctgt
     5041 tatcatagat gaaagcaaaa tagaccttaa tgttgttaca ataggatcaa tagttaaagt
     5101 taaggattta gaatttgatg aagaggttga atatactata gttggttcga cagaagcaga
     5161 cccatatgat ggtaaaatat caaacgaatc tcctgtaggg aaagcacttt taggtagagc
     5221 tgcgaaagaa gttgtggaag tgcaagttcc tgatggagtt gctaagtttg aaatattaga
     5281 aataagaaga taatttggag gaaaaaaatg aaaaataatc aacaaagcaa tgaagaagcg
     5341 caaattcaag aagaccttag tgaagtactt caagtaagaa gagataaact aaaaaaatta
     5401 caagagtcag gtagagatcc ttttaaagaa agtagatatg atagaactca ttattctatg
     5461 gatataaaag ataactttga ttctcttgaa ggtaaaacta caaaaatagc aggacgtata
     5521 atgagcaaga gaatacaagg gaaagctggc tttatagata ttcaagacca agagggaaga
     5581 atccaatctt atgtaagact agatgctata ggagaggaag aatatagtgt attttcaaca
     5641 tatgatattg gagatatagt tggaattgaa ggagaaatct ttaaaactaa aaaaggtgaa
     5701 atatctgtaa aggctaaatc tgtagtatta ctatgtaaat ctttacaagt tttaccagaa
     5761 aaatatcatg gattaaaaga ccaagaacta agatatagac aaagatatgt tgacttaata
     5821 gtaaatccag aagtaaaaaa tgctttttta attagaacaa aagcattaaa agcattaaga
     5881 gcatacttag atgatagagg atttttagaa gttgagactc caatattaaa tactatagca
     5941 ggtggggcaa atgctagacc atttataact aatcataata ctttacatat accaatgtat
     6001 ttaagaatag caaatgaatt gtatttaaaa agacttattg ttggtggatt tgataaagtt
     6061 tatgaaatgg gaagaatgtt tagaaatgaa ggtatggatt taaaacataa tcctgaatat
     6121 actgcaatag aattatatca agcatatgca gactatactg atatgatgga aataacagaa
     6181 aatgttattg cacatatggc tgaagttgct acaggaagta tgatagttaa ctatcaagga
     6241 acagaaataa actttacacc accttggaaa agaatgagta tggaagattg tgttaaagaa
     6301 tattctggtg tagatttttc tactataaat acagatgaag aggcactaga agttgctaga
     6361 gaaaaaggta tagagataaa acctggaatg agaagaggag aagtaataaa tgctttcttt
     6421 gaagaatttg gagaagataa attaatccaa ccaacattta taactcatca tccagttgaa
     6481 gtatctccat tatctaagag aaatgttgaa gacccaagaa gaactgatag atttgaggca
     6541 tttgctaata agtgggagtt agctaatgca ttttctgaac ttaatgaccc tattgaccaa
     6601 aaaggaagat ttatagacca attgagaaaa agagaattag gtgatgatga agcttttgaa
     6661 atggatgaag atttcttaaa agcattagag gtaggtctac ctcctacagg aggactagga
     6721 ataggtatag atagagtaat tatgttatta actaactctc catctataag agatgtatta
     6781 ttattcccga ctatgaaacc tattgataac aatccaaata aagaagaaga aaactaagaa
     6841 atattagcat tctactaagt tttaaataaa gtttagctat taaattagca ataaatagct
     6901 aaattttgtt taaaatgtca gtgtagaatg ctttttatat gcttcaaaga ttcttttaga
     6961 tatttaattt ataaattacc aacaaacgtt agaaattgat attagttttt gttgattgag
     7021 actttatatt tagaaaatat gattgaaagt aatttattat gaaaatattt agaataattt
     7081 atgagaaggt aaaaagaata atttatgaga atactatata taaaaaatta ttgggggaat
     7141 gtctgatgtt tcaagtaatt aattttttat gtttattttg tgttggattt ttggttcttg
     7201 atgttatgat atatttttgt aaggatgcaa tggatatttt tgagggtaca actaatgtta
     7261 ataaagttaa gagtataaat atttgggcag atagagtgag agctaattca tattcttatg
     7321 aaaataaaat tgcaaaatag gtaaaattat atatgcatgt attgtttgta atatcaaggg
     7381 ttatatctat tagttttaat tatatttatt aaaaaaacca aaagttataa ccttttttaa
     7441 aataaaaaac aaaaaaagta ttgacgaaat atttttaag
//
LOCUS       DAMKPG010000138         7348 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMKPG010000138 DAMKPG010000000
VERSION     DAMKPG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.144"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..151)
                     /locus_tag="PC621_003370"
     CDS             complement(<1..151)
                     /locus_tag="PC621_003370"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF4110483.1"
                     /translation="MEGFWRILKVEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRL
                     NSMSPL"
     gene            complement(181..570)
                     /locus_tag="PC621_003371"
     CDS             complement(181..570)
                     /locus_tag="PC621_003371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDF4110484.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(582..1042)
                     /locus_tag="PC621_003372"
                     /pseudo
     CDS             complement(582..1042)
                     /locus_tag="PC621_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1471..2481
                     /locus_tag="PC621_003373"
     CDS             1471..2481
                     /locus_tag="PC621_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110485.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDLIKDLNVY
                     KTPEPFIYLSINRISPQKILIIENKDTYITITKMLLEGKEILKNKIDTVIYGEGKKII
                     NSIKGLKEDITIEYLTNKENEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHM
                     IDKTKENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEE
                     YN"
     gene            2481..4100
                     /locus_tag="PC621_003374"
     CDS             2481..4100
                     /locus_tag="PC621_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110486.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            4118..4846
                     /locus_tag="PC621_003375"
     CDS             4118..4846
                     /locus_tag="PC621_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110487.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            4833..>7348
                     /locus_tag="PC621_003376"
     CDS             4833..>7348
                     /locus_tag="PC621_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110488.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLKDDINEAINNLKLDNHE
                     SYMKLQVLKTRKKDIDNFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQD
                     LENNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNL
                     ISKNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNG
                     LNILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFT
                     AEDLSIDVKIENYKKIIFEINNKIKSNENNEKNLSNKIKEL"
BASE COUNT         3304 a          788 c         1024 g         2232 t
ORIGIN      
        1 caagtggaga catagaattt aatctctttt gatatctaaa attattatag taatatatat
       61 aatcctctat tgttttgact aattgttcgt atgtatcaag ttttcttaaa tagtacattt
      121 ccacttttaa aattctccaa aatccttcca taggagcatt atcaatacaa cgagcaactc
      181 ttacatactt tgtatcatgc cttgtgttaa aagttttgat ttaaatacct tactagtata
      241 ctggaaactt ttatcattat gaaaaatagg ttttgcattt ggatatagct ctattgcttt
      301 atcaaaagtt tctaacacca agttattgtt atttgacttt ccaataacat atgaaactat
      361 acttctatcg tctaaatcta gaattgcgct taaataggcc ttcatcccat tggttaattt
      421 aaattcagtt acacctgtta accatttttg agaggtttta tctgcactaa attttctact
      481 taatacattt tccgcgatca cttctggcgt acatttaaca taactaaatt tcttttttct
      541 gcaaacagaa cttaagccta cggctctcat tagtctgtac actctctaca atctttaaca
      601 atttcaactc tttcatctaa tgctgttttt ctagtctcca taaagatttc tcccttcgag
      661 gtttctttta tctacttatg aggattatac tctttacccc aagcttttag tgaataaact
      721 gatctcattt tatatttttt tgagattgtg tcataagatc cttcaccatt tagataagat
      781 tttatagcat tcaatttaaa ttcttcacaa tagcataatt tacaactttg ttttattaaa
      841 ccatcatttc caaaagcttc ataatttcgc atccactctc tgaatgtacg tttatccaat
      901 atttaattct tccattattt ttattactgt attctcacct tttaaatatg attcaattgc
      961 ttcgattttt ttgctctgaa gatatcttag ttttagatag tcttacatat tttaactgtc
     1021 tcctaagatg agtataattc attcatcatc agttctatag aatacaccta gttcctactt
     1081 actgtaggaa ctaggactat atgaaaccca ttcatagatt gtagtttctt ctgttgcatt
     1141 atagcgcaga atagtaaatt catgtgaaat tttattttga atttactatt tatatataaa
     1201 tgaagagtgg tcttattttc aattaaaatt tggaacaatt ttgggttgac atctacaatt
     1261 aaggatttaa gtttttgtta tactaataaa taaaaacaga gattcaaaat atataaataa
     1321 tacttaaact agtgctgata gattttcttt aagttctagt ttttttattg tcattatcta
     1381 atatgtacta ttttatgtac agtcaatata atctgaaatt gtaaaatggt gtataatata
     1441 gttataagta aagaagggat ttaaacatgt atgattacaa aaataaaaaa gacttatata
     1501 taccttaagg aaatacaaaa aatatacaaa atcaatgagc ataaagaact agtacacata
     1561 ataaaaaaac taataaatga taataaaata aaaccaataa aaacctcaga tctaacacct
     1621 cagcatgagc aattatatac aaagtataga ataataaaac aagaaacaga agaagataaa
     1681 aaaatattag aagaaataaa ttacaaaata tgtgataaat taagtataga ttattataga
     1741 aaaaacttag tacactataa aaataataga gttgcaataa tggatttaaa tagttatctt
     1801 attaaaaaat ctgataatct atcaaagaaa atatcgatta acgaaagatc ttatgaaata
     1861 tttaaggatg agaaattcat aagtagtaaa gtaggaaaag aagtactaaa aaacttaaaa
     1921 atagatctaa taaaggattt aaacgtatat aagacaccag agccattcat atatttatca
     1981 ataaatcgta tatctccaca aaaaatatta attatagaaa ataaagacac ttacataaca
     2041 ataacaaaaa tgctattaga aggaaaagaa atacttaaaa ataaaataga tactgttata
     2101 tacggagaag gaaaaaaaat aatcaacagt attaaaggat taaaggaaga tataacaatt
     2161 gaatatctta caaataaaga aaatgaattt ttatattggg gagatataga taaaactgga
     2221 ttatcaatat acacttccct aaaagatacc aatgaaatta ataatataag attatttgaa
     2281 gttgcttatg agcatatgat agataaaaca aaggaaaata ccttgaggaa tatccccaaa
     2341 aatcaaaaag ataattttga atgtggatta gaaaatatta ataacctaaa cttaaaagaa
     2401 agtattaaaa atattttagt acaaggaaaa tacatacctc aagaagctct aaatagattc
     2461 gacttggagg aatataacta atggaaatga ttaatatttt agaaggctac aataagagag
     2521 taaactgtat aggatcttat tatcttattg caactcttgc taacaataga aataaattta
     2581 aagaatttga caacattcaa ttttacaatt tattaattca agttttgtgt tatatatttg
     2641 atagatctct tagaagaaag aactgtttaa gagatgatat taaggatttt attgaagaaa
     2701 taaatcgtat ggattataaa ataatgcttt cagaggatga cttaaaggat ttagcaaact
     2761 atataattaa tggattaacc aatagtggta aagtatatct atttacctac tattctttag
     2821 aacaagaaaa acatatagat gaaagtatta aaattataga agataaaaat gtaaaaataa
     2881 ataatcagga aagactatcc tactccttaa caacagaggg ttatagattg ttgttatcaa
     2941 caaaagaata tgatgaacta ttccaaattc aaatatcaca aatgattgca aagttaagga
     3001 tagaaaaggg tgattatcaa ggcgcaaagg aagatattta cgatataata aataccttag
     3061 agattcatca acagaaaatt gataattata taaaaataat aaaagtaaat atactatata
     3121 taaaagaaga aaaatattca acagtaatag atacaactgt agaaacactt ttagaagaaa
     3181 tggacaaata tgaagaatta aaaaaagaag taaaactatt aatcaaagat aaagaggact
     3241 acttggaaaa ggatgaggat atagacaaag aaaagataaa aaatataaga actcaactat
     3301 tacaaatgaa agaattaata gatggaatag ttttagcaaa gtctacagcc acaaatttgt
     3361 tatctagagt tcaagatttt agagaagaat ttaaagaaat acttaaaaaa ctattaacaa
     3421 ctccagtaat taaaagattc aatttcaagg aagaaatctt agatagactt gaaaaagaca
     3481 gtgatttttt attgataata agagaattat atatatcttt atttaaagta gagttgccaa
     3541 atcgatttaa tattggagtg gcatatcaag aacagaaact attaaatact agagaaattg
     3601 aaaatgaaat agttaataaa gacgagttag aaattattga aaataaagag aaggaggaac
     3661 gtataaatta ttgtgaaaat tactattttg atttttttaa atacttaatt aaatatggac
     3721 ttgaaaaagg acaatttaca ttagaagaat ttctaaaaaa ttatctgaaa tatgacaaag
     3781 ttatttactt agaaataact aaagactcca aattgatgag agatataata atttacttcg
     3841 ctaatcaaag ggaaagtatc aatatcaaaa agataatcag aatgttagaa aatagtgcct
     3901 tctcaccaaa tacagatttt caaattgaaa gattaataga acgtttatta gtagaagatt
     3961 ttgaactatt aaatagcgtt agccatatta aaagtaataa aatatttgaa gatgaaatga
     4021 aaataaatac tgaaataaat ttagaaacta tgactgcagt taagctagaa atagcaaata
     4081 taaaatttga aataatctaa atataagggg aaatacaatg aatatatctt taaatgacta
     4141 taaaatagcc aatgaaatat tttatagatt acttaaaaaa agtgagttaa aagaaaaaga
     4201 agctaaagaa ttatttttag cttatataaa taacagtaat gtaaggtatg ctttagaggc
     4261 aatttgtgaa gtatctaaaa taaaaataat attaatttca gataccttat acttaatacc
     4321 aaataatgac aatgatattt ttgggtttga ctatagaaga gaatctttat tagggaaaga
     4381 tttaaaagat agttatttag gatatttgat aatgacaata atattttctg aatttacaaa
     4441 tggggtagca ccagcatcat atattcaagt attaaactta ctggatttag tacaagaatc
     4501 cttagaacgt tcaatagctc gtgataatct agaagaaata gaaaaatcat gtgagtttaa
     4561 tataactatc ttaaagaact attgggaaag taaaagaaag tgggacgaat ctagtaagaa
     4621 aggaatagaa acaacatcag cagatttcca aatagggttt attagaaaaa taattaattt
     4681 cttaaaaaat gaaggcttaa ttgtacacat tgaggatgaa gataagataa aaccaactag
     4741 aagatttctt gatcttatga ataattattt tttagatgaa gaaaggaaag tattaataga
     4801 aaaactagtt ttaagaaaag gggaaagtat taatgccagt aattaataaa tacaaaatta
     4861 taaattttaa atttggtatg ggacaagaaa gaatacttag caacagaacc atggaactgt
     4921 atggagaaaa cttacaagta gaagcagaaa atacagcagg caaaactatg agtattcaaa
     4981 ctatcataca aagtatttgt ccactatcgg atgtagccaa accgtttact gcaatatata
     5041 ataaaaaaga accattatac tcaatgactg aatggttgct agatgatggt aaaacgttat
     5101 tattaacggg aataggattt gagaaaaagg ctggatcatc taaagaagaa gggaaagaaa
     5161 aaaaggacga tttatacaaa tactttatgt ttattataga agaatctaaa gaaatggagg
     5221 ttgatataga taatatacct ttattaaaaa acaatgacaa aggtataagg atagtacaac
     5281 ctttaacggc ctcacaagac tatataaaaa tgctaagaga taaatatgga aaaaacaaag
     5341 tacacttctt taataattac ttaagaaaac aacatagaga aaaattagaa gaatatggaa
     5401 ttaaccaaag tgaatggaaa gaaattatta taaaacttaa tagtaataac aaagaaggtg
     5461 gactatcaga ctttgttaaa gaacataata cttcagaaaa acttataaga gataaaatat
     5521 tacctttaat agagacaagc ttaacaacag aagaattcgc tccaatattg caaattaagg
     5581 aacaagtaag aaagtgtata aataatatta tagacatgaa tgaaaaatta tcagattata
     5641 aaaactatag agatttaaac attagagttg aaagtttaca aaataaaata aataggatcc
     5701 tattcataga agaagataaa aataatgtat taggagaatt agctaatcta tatgtacata
     5761 tagaaaataa acgcaatgaa ttaaatgaag aaataaaaca gtatcaaaaa atgaaagcat
     5821 caaaagaaga ggaatttaag atagtagaat atgaagaaga tagctatgag tattttagaa
     5881 aagaaaaaga gatacaagat actcaggtag aatttgataa agccaaaaat gaattaaatg
     5941 ataaaataga agaactagaa ttagatgaac aatatttaaa tatatcaaag ttcaaaagaa
     6001 aaaagaaggg gctagaagaa ggggaaaaag ataagataga aaaagaaagt gaaaaacaaa
     6061 gaaaagaggt aaatcaagaa gaaataacgg ctattataaa tcgtctaggt agcagtattc
     6121 attattgttt aaaggaagaa agtattataa ataatgaaaa acttaaagaa aatacaaaca
     6181 acaatatagc tatacaagaa aaaaagaatc aacttaatga tgacaaaagt aagctcacaa
     6241 aagtattgat aagtaaaaat tcagaaatta aatatataga aggcaaagaa cgtgatttta
     6301 ataatcaact acatgaattt aaacttaata acactgatat taaaggcttt atttctaata
     6361 attttttagg cgaagtaata gattatacta ggtatataaa ttatttaaat ataaaagtaa
     6421 aatcaataga agaaaatctt gaatcgaaag tcatgcaaat agaagaatcg aataaactta
     6481 aggatgatat aaatgaagct ataaataatc ttaaattaga taatcatgaa tcttatatga
     6541 aactgcaagt attaaaaact agaaagaaag atatagataa ttttaaagaa aaatataaaa
     6601 tcattaaaac aagatttgaa atagacaatg aattagataa ctctactaaa ctatataaag
     6661 aggtaaaaca agagcttaac aaaatgaaac ttcaagtaca ggacttagaa aataataaaa
     6721 agcaattaga aaaagagcta gaagatttac aaaattataa agatataatt ttcgaagaag
     6781 aaatattaac agatctagaa agaaatggaa taaaacctat tgaaggcatg aaatatctat
     6841 taaccttaga tggagattta gaatacaaaa ataatctcat atctaaaaat ccattgctac
     6901 catattctat aataattaca aataaagagt atcaaaaact gaagcaaata aacttaaaaa
     6961 ataaaactaa aatctcaatg cctatatttg taagagataa catagaagat acttttataa
     7021 ctacatctag taaccttgta attagtaatg gattaaatat actatcttct ttcgataaca
     7081 aagctttaga tcctgaaaaa agagaaaagg atatgaatct aatcaaagaa caaataaata
     7141 gtattcgaga taaagtggag caatataaag cagaaattga aatattagaa gaatttaaac
     7201 ttctaactaa taaaattgat tttacagctg aagacttatc catagatgtg aaaattgaaa
     7261 attataaaaa aattatattt gaaataaaca ataaaattaa gagtaatgaa aataatgaga
     7321 agaatttatc taataaaatt aaagaact
//
LOCUS       DAMKPG010000139         7228 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMKPG010000139 DAMKPG010000000
VERSION     DAMKPG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.145"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            93..986
                     /locus_tag="PC621_003377"
     CDS             93..986
                     /locus_tag="PC621_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4110489.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1164..1988
                     /locus_tag="PC621_003378"
     CDS             1164..1988
                     /locus_tag="PC621_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110490.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKHEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREVVPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2087..2695
                     /locus_tag="PC621_003379"
     CDS             2087..2695
                     /locus_tag="PC621_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110491.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            2799..2975
                     /locus_tag="PC621_003380"
     CDS             2799..2975
                     /locus_tag="PC621_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110492.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4271..5023
                     /locus_tag="PC621_003381"
     CDS             4271..5023
                     /locus_tag="PC621_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110493.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5013..7124
                     /locus_tag="PC621_003382"
     CDS             5013..7124
                     /locus_tag="PC621_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110494.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2681 a          958 c          958 g         2631 t
ORIGIN      
        1 ctaacatcaa acgataactt aatacatata aaaataaata aacaattaat aaaaattaaa
       61 acttcattat aaaacggaag gatagtgaaa aaatgcaact atataacacc ccctttaata
      121 caaacaaaca cttaatctat ttctttacat ctacgctagt attgaactgg atattcagtc
      181 ttacacctgc gttgctcgga attacaaata caatacttgg agaaatcatt ttttatcttg
      241 catatacatc accagctatt gtaggtctat tttttatttc tacaatctat ccacaaagtg
      301 caaaacaaga ttttatttat agatacctaa gctttaaaga aattagaata aaatggatac
      361 tattaacaat attatttttt gctttaactt ttattacttc gttaataatc ggaaattact
      421 ttaatgtaaa aacttctgat atagattgga tacacatagc agtgtttaga ccttctaaaa
      481 tagcttatat gctattagca agttttatat tgagtatact tagtcaagaa gctggatgga
      541 gaggatacgc cattgacaaa ttattagtta gatttggatt tataggctct tcaattattt
      601 tagggcttgt ttgtggaatc tggtatctag ggtcgtattt tacacctaat caaataccat
      661 ataatttggc acaatactcc ctatttgatg cttttttatt tataccaaac attgtacttt
      721 taaactttat aattaatttt gtatatatca atacaagtag aagcatttta tcagctgggt
      781 tagttcatat gatgtactat tttttcaaca ttcaattact tctacactac cctattaaat
      841 tatgtgtaat agctcaatat gtacaaataa tatttggttt aatatttctt gtatatatga
      901 tttcttctaa taaatttaaa cagaaggtaa attctgaaat tgaacagata aagtcggatg
      961 attttgaatt tgagttagac tggtaatttt aatatgaatt ataaattaag ataatctatg
     1021 atttgatacg attactactt aaatataatt tactctctat aatttattgc aacatctgga
     1081 aaataattgt atttccacaa tcgcttgaag tatataaaaa caaaatatat actaattata
     1141 agtgaaaagg aggtcatatt ttcatgttta aaataggaga ggtttcaaaa ctaacacaaa
     1201 tttctattcg tatgttgcgt tattatgatg aacttggtat tttaaagcct gcagaaatag
     1261 acaaatatac aggtcataga ttatactcag ttgaacagat ttcaatatta caaagaattg
     1321 tattattgag agattcaaaa tttagcgttg cagaaatagc aaatatcgta cataattgga
     1381 atgatgaatt tgtaattaaa gaattgaata gaaaaaagaa tgaaatacaa aaggaaataa
     1441 agcacgaaca acaaagaata aataaaattg ataaatttat agaagctata aactgtgata
     1501 aagatgaaat tcattacaat gttgtcttta aaaagattcc aagttataaa atcatatcac
     1561 ttagagaggt agtcccagac taccaatctg aaggtatact ctgggaaaag ttatctaaat
     1621 ttattaaaga agaacatata gaagtatcac gtcaacccaa caataatata gctttttacc
     1681 atgatgaaga agtcaaagat aatggtgtag atattgaagt tggaatggtt gtaaagaaga
     1741 ttggaaagaa caaatctgga tttatatatc gtgagacaga agaaattgat atgatggcat
     1801 gtactatggt ttatggtcca tatgaaaata tagctggtgc atatgaatcg ttctgttatt
     1861 ggttagataa aaatagtgat tatcaaatat caggtataaa tagacaaatc ggtcataagg
     1921 gtgagcataa tgaaataaat cctgaaaatt atcttacaga aattcaaatc ccccttataa
     1981 aagtctaatc gattagtact taaatactta agtactaatc acccattgac atcccaaaat
     2041 tttcatgata gtatctaaac gtgtattaaa catgtttaat ttttttatgg agaaactttt
     2101 aaggggaaaa attatgaaag cagattcaac aaaggaacaa ttaattaatg ccacaatcca
     2161 gttactatct tcacatagag atgtttcaag cattacggct agagaaattg tagctaaagc
     2221 aaatgtcaat ttagctatga ttaattatta ttttaaatct aaagaggagt taattaatat
     2281 ttctattaaa aaatttttaa agcgtcatat ggatgaatat aaggctacac atgatactaa
     2341 aaatttagca cctaaacagc aattaaggga aatactaatt gcattttttg attttatagg
     2401 tgaacatagc caatttataa agatttctgt accctatata ttgatacaag aagaaattgt
     2461 ttatccattt gagatactac ctttaattaa ggctcactat gaaaatcaga ataacaaaaa
     2521 aactgaagct gaatacagaa ctattgcgta tcaaataact tcatttattc agttgctttt
     2581 tcttcgttct aatgcttttt tcaagtatag tggtattgat attcttaatg ttgaacagag
     2641 aaatggttta atagattttc aattagattt attattggta gatattacaa aataatacta
     2701 tattaatttt ttatgtacat ttttttattt tatggtaacg tttgaaaata ttctcattga
     2761 ctaaaaatac ttgcattaaa taagggggag gaattttaat gttttttaca tttataggag
     2821 taatatcagc aattttattg ttgtgtacaa ttatatgtgg tttatggata aaacttaata
     2881 atcgtaataa tgactctaat tctcacttta ctttttcaat actttcaatt gctttttcac
     2941 tgttaattgt ggtggccttt ttagtaagaa attaatgtct ttgttatgct ggggtttatt
     3001 attatagatt tttattattg catgattttt actatattat agtattttta ttatattaca
     3061 gggtcttatt tattatagtg ttttgttatt acagcatttt attatattac agagtcctat
     3121 ttattatagg atttttatta tattgtaaaa tcttatttat tataggattt tgttatatta
     3181 cagactttta ttatattaca atctcttatt tattgcagtg ttttgttatt acagtatttc
     3241 tattatattg caaactctta tttattatgg tgttttgtta ttgcaacatt tctattatat
     3301 tataaagtct tatttattac agttctttgt tatactacag acttttatta ttacagcatt
     3361 tctattacaa tctcttattt attacagttc tttgctatac tacagacctt tattattgca
     3421 acatttctat tacattgcaa agtcttattt attacagttc tttattatac tacagacttt
     3481 tattattaca acatttctat tatattacaa tctcttattt attacagttc tttattatac
     3541 tacagacttt tattattaca gcatttctat tacattgcaa agtcttattt attacagttc
     3601 tttattatac tacaaacctt tattattaca acattttatt attttaacgt ctttactata
     3661 atatagtaat attttaatat ctcaaaatca tatcaatcac ttttcagcat agtctaaaat
     3721 acttccactt tctaaaatta tctatcatac ctatactaat aatacttttt aaccttttta
     3781 ttatacattt caattgtttt taacgaccta tttatttcca aaactccaat atattttctc
     3841 aattacattg tgttaataac ccctaaatcc tttcaaatta tttacaatac attccattaa
     3901 ataatcttac tgctctataa ttcttttttt ctttttaacc ctactataca ttatttacat
     3961 catttttttc aatacaacat tcgttattct ataacctttt tacttttact atataccttt
     4021 tttctaacta caccttcaat gttccacatc atattacttt agcacaacaa ttaataccta
     4081 aaaataaccc tctaaatcct ccaataatat aatcaattta actataataa taacctctca
     4141 cactatccaa acaatatatt ttcaaacatt acaaaaaaac atccaactat tacaatattg
     4201 ctcatatccc tcctcccctt tcaaatgcta tcattgactt acaagataaa tgaaagtgag
     4261 gatgatatat atgcttaaag taaaccaatt atgtaaatca tataaaacag gcaataaaac
     4321 atatcctgta ttaaaaaata tttcttttga aatcaacaaa ggagagtttg tcgcaatcat
     4381 gggttcatca ggaagtggaa aaactactct tttaaattgt atttcatgtt ttatcccaat
     4441 tgataatggg gaaataattc ttggaaataa taatataaga aatttaaaag aaaaggaact
     4501 ttcagaagtt agaaaccaac aactaggatt tgtattccaa gatttcatgc tatttgatgg
     4561 tcttagtgtc tttgaaaata tatgtgttcc acaaattatc aaaggtagtc aagtcttaca
     4621 aatggaaaac cgtgcaatta atctatgcga gctatttggt attgaacata tcagaaaaaa
     4681 ataccctgcc gaaatatctg gtggagaaaa acagcgtaca gcagtagcaa gagcactcat
     4741 gaacaaccca gatttaatat tagcagatga accaactggt aaccttgata gtaagtcttg
     4801 tcgtacagtc attgaatcat ttttaaaagc aaagcaagaa ttagatgcta ctatactcat
     4861 ggttacacac gatagctttg cagcaagctt ctgcgaccga gttattgtgc ttaaagatgg
     4921 aataatttat aaagaagttg taaaatcaaa ttcaagacat aaattcctag atgaattact
     4981 agaagtttta aaagaaatgg gaggcgctca agatgacaat taataaatta tacagcaaac
     5041 ttcgagccta taattggaag aactatacaa ctttattctt ttgtataata ctttctgtgg
     5101 tattagtaac ttcatatgct ctaatttatt ttagcccaac aatacaaaac atacttccaa
     5161 ctggtggaga tagcagaaaa caggctacaa tgatattctc agttgctata ttaggatgcg
     5221 ctgtctttac tacatacgct tcttccctct ttttaaaatt taaaagcaag gaaattggta
     5281 ttttcatggc tctaggaaca gataagaaga aattaaaaaa acttctattt tcagagatat
     5341 tgttaatttc aatagtatgc tgttttttag gtcttatatt aagtattcct gtatcttttg
     5401 gtatatggaa aatatttcaa ttagtgatta ttgataccag agaaatgaca tatcattttg
     5461 gaactcaagg attcatatat ggatttatat ttagtgtatt tgtgacatta tgcatattta
     5521 tactttctgt aaaatttatt aatcgtacaa atataattga tatattaaat gaccagagaa
     5581 tatctgaacc aatacaggat gtaaaggact ggtatggtat attaggtata atttttatta
     5641 tactaggtat ttttctagga tatatcgtac cttcactaac tataaacaaa ttaaattata
     5701 gactaccatc tgtatggagc tttacttatc tactctctct tattggaata tatatgatta
     5761 tgacatacat agttgtacat gctaaaagag gaaaacatcc agaaaaatac tataaaaaca
     5821 tcatatcaac tagcatgatg agatttatgg gaagacaaac agtaaaaaac atgtgtgtta
     5881 tttcattttt gattgcagga gcattatttg ctgtattcta tgttcctaat atggttgcag
     5941 gtatctatga tactcttaaa aataacccta tagattatac ttttacctac aatcaaagag
     6001 ataaacaggt atcaaaaaaa gatattgaac aattagcaga taaacacaat ataaaaattc
     6061 aaaactatat ggaaattcct tctatagaat taattgttga tggtgtagaa tcaatatatc
     6121 ataaagatgg aactatcgaa agggtatata tgaaaaaaag ctcctatgat atgttcttta
     6181 attctaaaga tttttccaag ctaattgaca aaccaataac tataaaaaat ggtgaatatc
     6241 ttgctgttgt aaataaagga aatgaaaatc aagattataa atataccaaa tttatatcaa
     6301 gcccagtcac agaaaaagaa atccccttta gatatgctgg aaaaataata tttaatggtt
     6361 ctttttttca aaatagaagt atgaacgcct atgtccttaa tgatgaggat tataaatcat
     6421 ttactaaaga tttatccata aaaaattaca ataattctat actatttaat gtagataaag
     6481 atacctatgc ttttgccaat gacttaaaga aagaaattat aaaaagaaca tctaaggaaa
     6541 tgggcatgtt cagtggttat gatgaatacg taaaaaaacg ttatgaatca actggaagaa
     6601 aatattttat ggatgaagaa tatccaagtg gtgaaggtca tttaaaacta gacccaaaca
     6661 acaatcagtt gtatcttaat tggaaatact atcctaactt taaaatttta caaagccaag
     6721 atatgataaa aaacatggca gtcttcttaa tgttattcat atatgtggca atcatatgtt
     6781 ttacagcagt aggaatcatt gcatatacaa ggggtgttac tatagctata aatcacaagc
     6841 aggtctttat ggatttaaat cgattaggag caaacaatga ttatataaag ttctgtatta
     6901 gaaatcaatt aaagaaagta ttttcatacc ctcttattgc aggaagtttt ttaatttatc
     6961 tgtttaattt tatgattatg ttaaataatg atggtagaat cgtcaatagt gaggtcaatg
     7021 ctcttttgat aaacttaggt tttataggga tttgtggagt ttatatgtac cttatatatc
     7081 tattaactta taagaaattc aaaaaaatta ttggtatttt ataataagga aggttgttag
     7141 atttttaaag taataaaatc attacttttt ataataagaa aagcattagc attttaaatt
     7201 aaaaaaggtt attagatttt tatgatat
//
LOCUS       DAMKPG010000140         7213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMKPG010000140 DAMKPG010000000
VERSION     DAMKPG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.146"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            133..1179
                     /locus_tag="PC621_003383"
     CDS             133..1179
                     /locus_tag="PC621_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF4110495.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            1287..2450
                     /locus_tag="PC621_003384"
     CDS             1287..2450
                     /locus_tag="PC621_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110496.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(2539..2919)
                     /locus_tag="PC621_003385"
     CDS             complement(2539..2919)
                     /locus_tag="PC621_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF4110497.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(2922..3581)
                     /locus_tag="PC621_003386"
     CDS             complement(2922..3581)
                     /locus_tag="PC621_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF4110498.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(3687..4661)
                     /locus_tag="PC621_003387"
     CDS             complement(3687..4661)
                     /locus_tag="PC621_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF4110499.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(4701..5048)
                     /locus_tag="PC621_003388"
     CDS             complement(4701..5048)
                     /locus_tag="PC621_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110500.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(5266..6222)
                     /locus_tag="PC621_003389"
     CDS             complement(5266..6222)
                     /locus_tag="PC621_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF4110501.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            6663..7031
                     /locus_tag="PC621_003390"
     CDS             6663..7031
                     /locus_tag="PC621_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF4110502.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         2701 a          862 c         1004 g         2646 t
ORIGIN      
        1 ccaaatgact taataatgtg aaaatataag acctaaaata taaattttat aaatacaata
       61 gttattgact ctctcattgt aagagggttt atactgaatc taatgaaaat ttatagtgag
      121 gagatagatt atatgagaaa aataattgaa attaaacatc attcacatga ttatgagtgt
      181 atgtggaatg gtatagaaga tttatatata aataaaaccg gtgaatgcct tccaaacgat
      241 tttttctttt tactgtcagg ttttggttca ttttgttata tgaaaacaaa taaagctgat
      301 ttgaaaagaa tggtagcttt gggagatgga aggacaaaac agatgtataa atttttagca
      361 ccaattgttg gatttgaata caaacatcat gagtttaaaa agtatgagca agcattaaag
      421 aaagcaaaat ctgaaattga tggtggatat ccagttattt taggagcttt agatatgtat
      481 tatctaccat attataagaa gctatatcat aaagaacaca ttccttttca ttatatatta
      541 atggtaggat atgatgatga aagaatatat ctgtatgatt gtggtagaac agaattatta
      601 catctttctt atgataaatt acgtgaaagc atgaattgca attcccctgg attaagcaaa
      661 gaaaatacta tttgcacaat aagaatgaat acttcaaaga ataaatataa cattgctaga
      721 gaagctattg ctataaagcg tgatatgttt ttaaatccat ctaaaggatt tttgggatat
      781 aaaggttttg aaaagtttat taaagagttg ccacagtgga acactgaatt ggataaagat
      841 gattatgata agattttgac caatatggtt atgttttttg gaacagtacc aacaattcca
      901 aatgcaatca aaggaattaa agaaccagat actgttcaat ttaaaggtgg atttgataaa
      961 atgagtagaa tgctcaacag tattggggag gaatacaaaa acgactcttt tatacaagta
     1021 gctgatattt ttgataaaac tgctattata attgagaaaa tatccaatat tataatagat
     1081 tatttaactc aaacatgtga tgatacagaa caacttccgt tattgttctc tgaggtctta
     1141 gagcatatga aaacaggata tttaatcttg gatgtataaa aaatatattg ttgaccctaa
     1201 cattacgtaa tagtgtaata tgaaaatata gcttgaatat attagtaggt attgatggct
     1261 gtaaaattaa aatttgaggt gtggatatga gaacagtgaa acaagtttct gatttaactg
     1321 gaataagtgt gagggcatta cattattatg atgagatagg tcttttaaaa ccaaataaaa
     1381 ttacagatgc aggatataga ctttatgatg atgaatctat taaaactctt caacaaattt
     1441 tattttttaa ggagattgat attcctttaa gagaggttaa ggaaataatg tcaagccaat
     1501 attttgataa ggttgaagct ctaaaaaatc aaaaaaaatt acttatttta aaacgaaaga
     1561 gactggatga attgatagag cttataaatc aaactctaag aggagaagga aatattgatt
     1621 ttaaagagtt tgatatgagc gagtatttta atgttttaga agaatttaaa agagaacata
     1681 ggaataagat aattaaaatt tatggtagtg tagaaaaata caatgaatat atagaaagag
     1741 ttaagtctaa tgaagagaaa attgctaaaa tggctataaa acaatatgga actattgaga
     1801 agtttgccca agctataaaa acaaatttta gcagtgatat attgaattta ggagaaaaat
     1861 ttgatagata taaaaatgat tgtttaaaag aaaaacatcc aaaattaaaa gaattatata
     1921 gaaagcttgt agaagattta agtaagaatc cttcttcaac agaccttcaa gaaatagcaa
     1981 aagagataac agatatatct aaaaaagatt atgaaatttt ctctatgtat actggtgatg
     2041 acaactggta ttatatgatt caaaattatc tagtaaatcc tatgtggata gaagaagtag
     2101 ataaaaagta tggaagtgga gcaggcaagt ttattggaca agtactaaag acttatctaa
     2161 gagatagaaa accaaagata aatacattgt atgaaaaact tgtagaagat ttgagtagag
     2221 attgttcttc aagggaagtt caaagcattg ttgaagaaat agacaatgaa atgaaaagaa
     2281 gtaatgaatt ttataaaata gacaatggtg aaagatactt tgattatatg tcagagcttt
     2341 atttacaaga ttctaactat ataaaagtaa ctgataagaa ttatggtgat ggtgcatcta
     2401 agtttattgg agaagctttt aagatttatt ttgataataa taattgttga aaatttggaa
     2461 gtaaaaaata tgtaagtttt ataaaagcac aaaaataagg atgtagatta gctccatcta
     2521 tatccttatt taaagttatt aaaatatata gtttacatca aacttaacac tttcaataat
     2581 atttcccaaa tatgttttta tactttcaac ttcttctaaa gattttaatt tattttcagt
     2641 ataaattgct ttgtccagaa tattaaacct taatcctatt tcatcaaagt actcatgatt
     2701 taaaactttg taccaatggt ttttttcttt attccaactc gtagatattg aatcagcttc
     2761 tttttgagct ttttcccaat cttcattttc tatagatttt tctaaaacag atatattgtc
     2821 tttatatcct tctgtaaaat catatagttt attgttaacg taaaatccaa ataatacaaa
     2881 taagatagtc catagtataa caaatgttgt agatttcaat attaatcacc ctttttattt
     2941 tgaataaata ttttgtcatt ttcatctaaa acacaaataa gaacatcttt aaaagtttct
     3001 atgtgattag attttaaaat acccattaac cagttagtat ctttttgagc tatgtctaga
     3061 tttttattta ttattctacc atccagtata agtgggatag gtaaatgatt aaaagctctt
     3121 ggaggagtac tattgtagct tgatgctggt acaacactta gattaccatc agtctccaaa
     3181 atagcatatt gtacatcttt taggttgaag tatccttgaa tcctaagttg ttctaataat
     3241 tcatctatac taattctttc ttttttaagc tctttatata caatttttcc tttatctatt
     3301 aatacagaag gtttacctga taaaaatgaa cttaattttc tgctttttaa tgataagaat
     3361 gaaataagag tctgcatgat tacaagacca gatattgctg caacaccatt tatgattggt
     3421 atattattat tttccattgg gacagatgca acttctgcaa ttaatagtgt tataactaaa
     3481 tcaaaacagt tcatttcagc tatttcacct tttcccatga ctctaagtgc aattagcact
     3541 gttatgtata atataatact tcttattaat actactgtca tatctatcaa ttactccttt
     3601 aaaaaatatt tacacttagt tagtatatcc tatataaaaa atttaattca aagtataaaa
     3661 aaccttaaat aattggattc aactatttaa ggtttttttg ataattttaa tattaaattt
     3721 tttatggatt ttctgttact tttatttatt ttaataacag attcaacaaa actcttttta
     3781 tctcctaatt ttaagataaa atcaggatat tcttcaaatt cattgagttt tattaatttg
     3841 aaagaagaag atagattttt aaagttaatg attttagagt tattcactaa tacacttctt
     3901 tttttaaaat aaatttcttt tttacttata tttagtatag ttaatatttg tttaagatat
     3961 attattatat cttctggagt tctatcaaat ttgagttcaa aattttcatc ttcaaagtag
     4021 ggaatataat ataaaggtgt tttgagagca ggtaatttat tatataattc ttcaagaata
     4081 tcgatatttt taggggagtt aaagaaaaag catttacata cgttcattaa atccacagca
     4141 tctgaaagtg agttatgttt aatatctcct tctacagaaa aaattttttt catattcagt
     4201 aaagatattt gcttagataa gattctatta ttgtatgtaa tatgttgaga tataaaaggt
     4261 tgaatgtcaa tcatcatatt aaataactct aagcatttac ctgtatattt atatactcta
     4321 aatgaagtta gaaggccagt tatatcaaaa tcaccccaat tatatattgt aacatcatca
     4381 aatttacaaa accatttata aaaatcatcc ataacatata aaagattagg agcattattg
     4441 atatctagtt ggttcaattt ggttaaattt ttacatttat ttgaaagtat tttattgtat
     4501 tttggcttaa ctaaagagta ataattatct attgtattaa attcgttatc aactaaaact
     4561 ccaccaatag aaattaattc cgcaccatta tattcttttg gtttattttt attatcatct
     4621 agtatattaa attcaaagtc tataaataac ttgtatttca taaatctaaa gcacatcctc
     4681 aggtaaatta tttattgagc ctaagcatca taagaatcaa tagatataat gactctaaac
     4741 atcaaaggtt cattacaact tttatttatt tcataattta attcaaataa atcttttaag
     4801 atataattta taagtattct ttcccaaccc atttttaaat taactttaga gtctattaat
     4861 atattttcta tctttttaaa tatttctata tttgctggta ttatactgag ctcaatatta
     4921 cttttaaacg ggttagactg taaatctgtt ataagtatat tttttaattc ctctgtaagt
     4981 tcagaaatta aactatcacc tatgtatttt tttatgattt cctctctaat ttcttttaaa
     5041 tttagcaata ttagtacctg ctacttacat agtagctttt cctttccttt taatactatt
     5101 attcattttt gaatcttatt tagatatatt ttaagattaa aagtctttag ctatataatt
     5161 ttttacccat ttttaactta tattaacata ttacaggctt atatctccac cctgcttaca
     5221 ttgaggatgg agtatattgc ctattttgat aaaaatgata ggttgttatt tgcctattcc
     5281 tttttctgat aatttttcta acatatcttt ggtcatttta tctaaatcat aactaaactt
     5341 aaagttccat tcttttactg cactactact gtctagagag ttaggccaag aatcagctat
     5401 actttgtctt acagggtcta catcatattc tatcacaaaa tcaggaatat atttttttat
     5461 agaatcagct atttcttcag gagaaaagct catagcagta atattaaaag catttctatg
     5521 gatgagttta tccgctggag cttggattaa atccactatt gattgaagtg catcaggcat
     5581 atacatcata tccatttttg taccttctgc tatgtaagat ttatatctct tattttttaa
     5641 tgcctcataa tatatatcaa ctgcataatc tgtagttcct ccaccaggag gcgtaacata
     5701 agaaataaga cctggaaatc taacacctct agtgtctact ccaaattttg agtgataata
     5761 atcacatagt aattctccag aaactttagt cacaccatat atagtattag gtctttgtaa
     5821 tgtatcttga ggagtcatat tttttggaga attttcacca aaagcagcaa ttgaactagg
     5881 tgtaaaaaat ttacaatcca attctttggc tatttcaagg ccatttatta atccattcat
     5941 atttaagttc caagcttcta ggggtttact ttcagcaact gcagaaagta aagatgctag
     6001 atgaattata gtatctacat catatttttt tgcaatttca aaaaaacgat tcttatctgt
     6061 aacatctaat atctcaaata taccagattc acatactgga ttaccttctt ttactctcct
     6121 tgaactagcg attacatttt gttctccata ctcatttcta agctttattg ttaattctga
     6181 acctatttgt cctaatgcac ctgttataag tatttttttc aattttacaa cctcctaagg
     6241 gtttaattta tgtagtacta cttattttta ttttagcaaa tcttttgttt aaatacaaag
     6301 aattaaagat aataatatac gacttaattg aatgaagtgc agtaaattcc taaaaattta
     6361 aagttgagat ataatggtct attgtataac aataaaagac tagattaaat tattatatag
     6421 attgtgtgac taagtcacag aaagtatagt taatttaata tataataaaa gcatatcaag
     6481 taaagcttta aattaaagta actttatata aaaagtaata taaattgata aaattataaa
     6541 tttatttata gtgaatatag gttggatttt atagatacaa aaatagtata tatgatatgt
     6601 agaccattag catgtttaaa gtagcaaaac atataaaata aaatttgaaa ggaagtataa
     6661 aaatgacaag accatcaata tatcatagcc aaggatacac ttgtgcagag gcattaataa
     6721 agtcttataa tgaggaacac aacacagata taccaatctc aataggaagt ggtatggggg
     6781 ttggaatgaa tgttggaagt gtttgtggag ctgtaaatgc tgctgttgtt ataatagggt
     6841 atttaaatgg gcgaaatagt aatttagatg aaaatatagc aaggaagtat tcaagagagc
     6901 tattgaataa agtaagaaat aaatacagct cagaaatatg tgtatctctt aaaaaaaata
     6961 aagtaacttg tgaagaaata ataaatttca catatgatgc tctaaatgat atgctaaaaa
     7021 atcaaaaata attctaagaa acaagtattt tacattcgat agaatacttg tttttttatg
     7081 atatttttaa taggtattag tttattataa tttttaaatc aattttatta tattttaatt
     7141 gaaataatct actttgaaaa ttactaactg acaaaagaat atgattatag tatagaaaaa
     7201 atgtaaatta ttt
//
LOCUS       DAMKPG010000141         7183 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMKPG010000141 DAMKPG010000000
VERSION     DAMKPG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.147"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            51..1454
                     /locus_tag="PC621_003391"
     CDS             51..1454
                     /locus_tag="PC621_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110503.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNKYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1674..4151
                     /locus_tag="PC621_003392"
     CDS             1674..4151
                     /locus_tag="PC621_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF4110504.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4166..4852
                     /locus_tag="PC621_003393"
     CDS             4166..4852
                     /locus_tag="PC621_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110505.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5125..5958
                     /locus_tag="PC621_003394"
     CDS             5125..5958
                     /locus_tag="PC621_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110506.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            5996..6457
                     /locus_tag="PC621_003395"
     CDS             5996..6457
                     /locus_tag="PC621_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF4110507.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6610..7035
                     /locus_tag="PC621_003396"
     CDS             6610..7035
                     /locus_tag="PC621_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4110508.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2774 a          785 c         1127 g         2497 t
ORIGIN      
        1 tataagcaac aatcaaacta aaataaaagt ttaagaaaag agttgattaa atgcataaac
       61 ctagttataa aacagtattt cattcataca ttattttttt catgattttt atttgcttaa
      121 tcctgacagt aataggtatt ttcttatcac ttattactat tcagaaacca aatggggaat
      181 ttattcgtag tgactggccg aaaaaattta cagaaaactt taatgaacaa atcatattta
      241 tagataataa gccacaagta aaacagtctg gattagaaaa gcttcgagaa aatcaattgt
      301 ggttgcaaat tttagattct agtggaaata gagtttatgg gtttttagaa ccaaaagagc
      361 ataaagatta ttattctaat tctgaattat taacattagc taaccaaaat aattcaagta
      421 atggaactgt atactttaat acaattaaaa ataataaaaa tgactatatt tatattatat
      481 attttccatt aaatatatct aaagtgaata tgttttttga tggaagtaaa tttgtaggtg
      541 gtaagtcaat tgtattttca atcataggga ttatattttt aataattttg atttctggaa
      601 ttgcttatgg ttactgggta acaaagtgca tatcaaatat tacatctgct gttagtgata
      661 tttcaaaacg aagctatctt ccagttaata ttaaaggtgt ttttgctgat atatataata
      721 gtttaaatat tttaaatgaa gaaattcata gaagtgatga gatacaaaat aaaactgata
      781 ttatgagaga agagtggatt tcaaatataa ctcatgattt aaaaactcca ctttcaccaa
      841 taaaagggta tgcagagctt cttacagata gtagtactaa ttattcacct gaaaaagtat
      901 ataaatatgc ttcaatcatg ttaaagaata tagaatatac aaatacactt ataaacgatt
      961 taaaattaac ctatcaatta aaaaatggaa taattccttt aaataaaaat aaatatgatt
     1021 tagtaagatt cttgaaagaa ttatccattg atattttaaa ttatccagaa tatgaatcaa
     1081 atatagttga atttaagagt aatatagata atattaactt tgattttgat tgtacactat
     1141 taaaacgagc ttttagtaat cttattgtca atgcttttac atatggagat aaaaatacta
     1201 aagtttgtgt acatataaca ttaaatgaaa gaattgaaat cattatatct gataatggaa
     1261 aagggatgtc aagagaagaa gcatcaaatt tgtttaatcg ttattataga ggaagcaata
     1321 caaaacaaaa aatagaagga actggtttag gactagcaat aacgaagcaa attattgaag
     1381 ttcatggagg gaatatatct gttgaaagta tacctggatt aggtagctca ttttatatca
     1441 cctttcctat ttaattaagg ttaattaagg tcaaataaaa aatatattaa ggttgataga
     1501 ttattatatt tatatagtag tttatcaatc ttttttattt aaggaggtga tttgattaaa
     1561 aatattataa atagaatgat aaaagtatgg aggagtctat tagagattca atttggatta
     1621 aagtataaaa atattatact gaaatatctt agataaagga agaaggtgta aatttgaaaa
     1681 ttatattaaa gtatattttc acaaatatta aagaacgaaa aattagaaca gcagttatgt
     1741 tactgtccat agttttatca acagttttac tttttgtttc attctcaatt ggattatctt
     1801 atgaaagtgc acaaagaaaa atggcaaaag gtatgtatgg tacagcgact atatctgtcc
     1861 aaagtaaaaa tccagatgta ttaaccaatt tagaagacat acctgattta aatgctataa
     1921 aatcaaaggt tggtgtattg gaaagctctg ctatttataa taaaggggga tactatgaag
     1981 aatttagtct tatatcagca gacttaagtc aactaaataa aatcaacaag ccacgattag
     2041 aaaatggcga tagtattact gatttttctg gtgataaaat tattcttcca aatcgcttta
     2101 catcaaaata taaaataaaa aaaggtgatt ctattacttt acagatttat ggaaagtctt
     2161 atacttttca agttagtgat attgcatctt atgatacagt atttttgagg aatacaagag
     2221 gtgtaaatgc actgctaccc aaagagactc tatcaaaaat tataaataaa ggcagtggat
     2281 atacaagagt tcttatagag tcagaagaag aaatgacaga aaatctagta aataaattat
     2341 ccgaagaact ttccactgaa aaatatatgg tatcaaatac tataaatgaa actaagataa
     2401 tttcagatgc tagacaaaag acaatgcctt tctttttaat aagttttttt gcattaaccc
     2461 ttagtatatt tatcatatac agcagctata aggttattac attagaacgt cttcctttta
     2521 ttggaacatt tagaagtatt ggtgccaatg aaaaaactgt aactcgtata ttgatgcttg
     2581 aaagtatatt atacggaagt ataggaggat taattgctat tcctattggt gtagttgttt
     2641 taaatttaat gcttcatggg ttaggaaatt ctctcgaaca aggaatatca attccagtag
     2701 taatatcacc cattggagtg attatatctg ttatagtagc aataatcgtt tcttcattta
     2761 gtgcatatat acctgttaaa aaagctagcc atttgccaat aaaaaatatt gtactaggaa
     2821 cagtagaaga aaagaatgtt tcaaatcgtt ctatactttt tataggaagt ataatgttta
     2881 ttttatctat tttacttcca agaatatccc ctgaaaatac attgtatcta gctggtggat
     2941 tttcattact aggattaatt gtagctacta ttgtgttgat tcctcttatt actgatatca
     3001 tgtctatagt ttttgagttt gtttataaaa atattttagg gaatgagggt aaattagctg
     3061 ctagaaatat gaaaaataac aaaaatataa tacagaatat cactttacta tttataagta
     3121 tttctgcagt tattgcaata agtgttgttg gtaactttgt caaaacttat ataacagatg
     3181 tgtttcgtga cgcagaatta caaggttttg cagatggaaa gatgaatgaa gagtttattg
     3241 aaaatgttag acatatggat ggaattaaaa aaatattacc tctttatgtt atgaataatg
     3301 agatttctgg taatggtgta acattatcaa gactagaggg tacagataat ataaaactat
     3361 ataactctat gtttggaatc aattatacaa attttgagat aaaaaaacaa gtaatagaag
     3421 cttttaatga taaacgttct gttattttga atgaagatac tctaaaaaaa gtaggcttat
     3481 ctataggaga tacaataact ctatctaatg ataagtatga tttttcttat aaaatagtag
     3541 gtagttttaa atcccgtgca aatgatgtag aagctgttat accatcacat tatgcagtta
     3601 atgattttga taaaactaat tatggttttt tagtatatac agcagtcaat ccagatgttg
     3661 ttatgattca gataagagat ttatttggag atacatataa ttggagtcgt acagttgaag
     3721 aatttaataa tgattcttta aatactataa gtagtttttt gtctcctatg aataagatga
     3781 cctatttcat ttttcttctt gctacagtag gtattattaa taatctactg attaattata
     3841 ttcaaaaaaa acgttctata gctatgtata aatctatagg actaagtgat aaacaaaata
     3901 taaaagtgac attgattgaa gggtttactt caggtttact tggagctgtt attggtattg
     3961 tgatatctat acttgagatt caaactatct ttatagttgc aggaccaaaa atttcaatga
     4021 aaccagattt agattttaaa acatttatta ttgttgggtt attgggaatt atagtaactt
     4081 taataggttc gatagtgcct attataaaag ggaagaaaat gaaactgata gaagaaatta
     4141 agtttgaata attatggagg ttaatatgaa aattacacat aatagtttag ctgtagaagc
     4201 aaaaaatatt atcaaagaat ataaaattgg aaatacaaca actcgagtgt taaaagaggt
     4261 atctttacaa gtaatgaaag gtgaatttgt ttctatcatg ggtcaatctg gttctggtaa
     4321 aagtactctt ttatatattt taggtggttt agatactcca acgagtggaa aagtgtatat
     4381 gaatggagct gatatatcac attttaatga tgagaaaatg agcataatac gtcgtagaaa
     4441 tataggattc gtatttcaat tttataactt aataccaaat ttaaatgtgg aggaaaatat
     4501 aatgcttcct ttgttattag atggtaaaaa tttaaaagat tataaaaatc aattagatga
     4561 aatcttagat atagttggtt taactgatag acgtaaacat acaccacgag agttatctgg
     4621 tggtcagcaa caacgtgttg ctatagctcg agctttagtt ggaaaaccag agattttatt
     4681 tgctgatgag cctactggaa atttagatag taaaacaggg atagaaataa tagatttact
     4741 gaataaaata aacagagaca atggacaaac tataattatg gtaactcact caccagaagc
     4801 agccaaaagt agtagtcgta ctattacagt ttcagatggt ttaattgtat aaggactaat
     4861 tattgtattt gagataagca tttttaacta tataaattag acatatgtaa agtggatata
     4921 agttaaatac tataaatgtg ctataatatt aaaataacag ataaaagtac tatatttttt
     4981 attgattatt ttaatttatt tgtttattaa atatctaatg agtattcata ctatctctat
     5041 tattaaaagc agttaatgat atatagagat aatatggatt cttcttactt taataaatat
     5101 tcacgatagg acaggatata atatatgtta aaaaaagaaa gtattcattt tataaataaa
     5161 aataggtcac ttatagtttg tgaagacaat tttgtttatc tcttgccttg tatgcaatta
     5221 aaaaattgga tatcgaattt tactatatcg tttccaaatc aaacaataat ttctgataat
     5281 tacacgatta ttcctcatgg aagtgtaact ttggtgtttt tttatgatgc cacagggtta
     5341 catagtctat tatttggtcc aactactaaa ccaaaaacag taggaaatat tgctaataga
     5401 tgtgatgtta tactaattat agaatttcaa cctgctggtt ttttcccgct tataggtata
     5461 cagcaaagtg aacttattga taaagttgtt ccattttcta taattaatac ttcattagat
     5521 ttagaaatta aaaaaatatt taatgaatct ttatctatag ataaattgat tttaaagttg
     5581 gaagaattat tgatttctaa tattaaaatt gaatactcat atgagtttat tttggcaatt
     5641 cagttgatta ttcaaaactc tggaaacata tcttcacaag aaatttctaa aaaagtattt
     5701 tatagttcta ggcatttaaa cagactcttt aatcagtacc tagggttgag tatgaagtca
     5761 ttttctcgtt tggtacgtat taataaatct ataaaacttc taaacaataa taaaacttca
     5821 ttgatgtata tatgtaatga attaggattc tatgatatac cacattttat taaagatttt
     5881 aaaattgtct gtggaataac tcctcaaaaa taccgagcta atatgtctga tttttacagt
     5941 gaaattgcaa aattttaagt tataatactt ataacaaaga aaggatagtg ataagatgaa
     6001 atttggagga gtattgctaa ttgtcaaaga cataaataaa tcaaaaaagt tttatgaaga
     6061 tgttatggag caaaaaatta cattagattt aggagaacat gtatcatttg aaaatggatt
     6121 tggattacaa agtaattata aagctataat tggatatgat ttaccaatat atcaacaatc
     6181 aaataacttt cagttgtatt ttgaggttaa tgatatcaaa caatgggaaa gtaagataaa
     6241 tagaatagga aatattgaat ttttgcatta tataaaagaa tacccatggg gtcaaagaac
     6301 ttttaggttt tatgattttg ataaaaatat tatagaaatt tcagaaagta tggaaagcgt
     6361 cataaagagg cttttaaaac aaggtttagc tttagaagaa atttcaaaac gcactatgta
     6421 tccagttgaa tttattacac aatttcaaca attataaaat attaaaatat ataatttttt
     6481 ctttatattg caaaaaacaa aggcttgggg cataatagaa aaatacttga aataaattac
     6541 atacttaatg tttgaattta gcttaaaaat gaaaagacga agaaatttat acttggaggt
     6601 aattacgtta tgcatgatga actgaaaatc aaaatgtact catttacgat ggattgcaaa
     6661 gacccacatg aattagcaaa attttatgca gcgttactca attgggaaat agcattttat
     6721 aatgaagaat atgcatgtgt tggtgctcca ggaacaaatc agggaacata tcctggtata
     6781 acatttcaac gaaattctga gtataaaccg cctgtgtggc cagaaaaacc tgaatctcaa
     6841 cagcaaatgg ctcatatgga tttcgctgtt aatgatttag aagaagcagt tcaatatgaa
     6901 attcattgtg gagctacaat cgcagaggag caatttactg atgactggag agttatgatt
     6961 gacccagctg gacacccttt ttgcttatgt cagatgaaat caattatgga aagtagccat
     7021 ttttcattgt tatagaccaa aatattgttt attaattaca aaaaagcaca acagtatata
     7081 ccgttgtgct tttacttatt atacatgaat acactaaaaa atataaatag tagagcttta
     7141 tcaataatga taatttttaa aaaatagtat ttttttattg aat
//
LOCUS       DAMKPG010000142         7072 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMKPG010000142 DAMKPG010000000
VERSION     DAMKPG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.148"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(151..963)
                     /locus_tag="PC621_003397"
     CDS             complement(151..963)
                     /locus_tag="PC621_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4110509.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(1333..2235)
                     /locus_tag="PC621_003398"
     CDS             complement(1333..2235)
                     /locus_tag="PC621_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF4110510.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(2285..2746)
                     /locus_tag="PC621_003399"
     CDS             complement(2285..2746)
                     /locus_tag="PC621_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF4110511.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(2798..3253)
                     /gene="def"
                     /locus_tag="PC621_003400"
     CDS             complement(2798..3253)
                     /gene="def"
                     /locus_tag="PC621_003400"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF4110512.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(3225..3488)
                     /locus_tag="PC621_003401"
     CDS             complement(3225..3488)
                     /locus_tag="PC621_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110513.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(3585..4301)
                     /locus_tag="PC621_003402"
     CDS             complement(3585..4301)
                     /locus_tag="PC621_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110514.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(4298..4975)
                     /locus_tag="PC621_003403"
     CDS             complement(4298..4975)
                     /locus_tag="PC621_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110515.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(4972..5817)
                     /locus_tag="PC621_003404"
     CDS             complement(4972..5817)
                     /locus_tag="PC621_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110516.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(5931..6521)
                     /locus_tag="PC621_003405"
     CDS             complement(5931..6521)
                     /locus_tag="PC621_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110517.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            6810..7043
                     /locus_tag="PC621_003406"
     CDS             6810..7043
                     /locus_tag="PC621_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF4110518.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         2514 a         1099 c          661 g         2798 t
ORIGIN      
        1 ataataattc aagtattgat ttttatataa gaaaatttgt aatgtactaa aaataacata
       61 aaagtttcca tttttcaata tgtaagcttt tcttatgtta ttttattgtt aaaggtttca
      121 agtttttatt tattcatttt aaaaaaatta ttatttttgt aaaactaatc ccatagtcat
      181 actatcgtca gaatatgctt cacccttaac attactaaaa aactcaacag acttaaatcc
      241 aatattttca acttctaata agaaactttc ttttgaaaaa tagtgattcc aaacataaaa
      301 tacattttca tctttttcgg ttattacaag agtttgttct aatgtattat aatcattgta
      361 tttacaatta tcttgtagac acatatattt ttcattactc caaaatcctc catcttcatt
      421 tatttcccaa tatttaattt cctcaaaatt attgtattta tttattgtaa atacatctaa
      481 tattaatttt cctccaggtt ttaaactatc ataaattttt tccattaata accttctatt
      541 ttcagttgac aaagctccat aatcacaata aattaaagta gccaaatcaa attcatcatt
      601 atagttcata tttaaataac tttgaaacaa gtaatttatg tttaaattag tctccttatt
      661 tctattttgt gcatagttaa tagaacgttt tgagaaatca attccagtta ccttataacc
      721 tttttgtgca aatttctctg catataaacc tggaccacaa cctaaatcaa gtaaattagg
      781 ataattaact ggatttgcaa ctgtaactat ccaatttaca gaatcctcga taaagttaaa
      841 atttctactt gctccttcaa aattagtgtc taaatgtgct tttaataatt gttttgaaat
      901 atactcatca tcccaaaaat taatttcact ttgtttataa agctctggtc tttctaaata
      961 catacataat ttgtttaaca aaacatacca ctccttaaaa tttattttta aaataaaaaa
     1021 accacagagt ttactctgtg gtttaacttc aaaaagatga gtttaaataa gagtaaaata
     1081 gaaccaaata tttttaagca caacaataaa actatagctt tactttgttt gcttattaaa
     1141 tttgatttta ttcactcttt tccagcaata cttatatcaa cgattttatg atatcaaata
     1201 tcaccgaaca acatcatcct ttcatatttt tattttatga atcaattata tatttaaatt
     1261 ttatatttgt caataaattt taattttaat aaattttaat catcatagta aaaaaaaatt
     1321 ttttatataa aactaatcaa aaaaagatat ctgtccatct cctgagtttt ttaacttttc
     1381 cttcattatt ttagagtaac tgcttgatag agaatattta tatctaagtt cattaaccat
     1441 tttatacaat tgatttttat actccctatt aagtgaacct gcactatata gtattgataa
     1501 tttatcaaat aattcaggaa actctttttt aacaaagtca aaaaaaacac ctctagttat
     1561 accccttaag tataaagtcc caggtaaaac ataatgtaca ttacattctt ttgcatgtct
     1621 aaatagcgaa tttatattat caaaatcatc tgtaatatat ggtataattg gcataatatg
     1681 tagaccaact gatgcgttag tttttctaaa ttcttttaac attttaaatc gtctcattga
     1741 atccacacca tttggctcaa taagcctttg tgtatcttca tttactgtgg taattgtaga
     1801 tgctacattg atatatgtaa tcctagatag cttatcaatt aaatcgtaat ctcttaaaat
     1861 taaatctgac tttgtagaaa taatagctgg tgtcttgtat tttatcagca ggtttaatat
     1921 ttctggcata attttatagt cagcttctat tggttgataa ctatctgtaa ctcctcctat
     1981 attgattact tctttcttcc atttactgct tttaagttgt ctctcaagtt tttctactat
     2041 atttgttttt acataaatct cttcaaaata attattggag ttaatgtact tatgagagta
     2101 aatagcataa caatatttac atccatgctc acatccacga taaatattta aatcccattt
     2161 atatggcata ctccttttta atttattaca agctatttca caatttattg gaatatattt
     2221 tattttatta tacaaataat cacctcttta tatacaataa tactataatg ttaatctata
     2281 gtaattaaaa catgatattt ccagaccaca attcataatc tttactatat ttacaaagta
     2341 atatattcca ttcatttatt ggtattcctc cacatgttgc tttttcttct aaattatata
     2401 agcattgctt taaatacatt ttattatgcc aatcatcata taaattttct aaatttaaac
     2461 tatgttcatt agatatatca tttgcaaaat ttctattttt ccattttaat atacttgtat
     2521 atttaagttc attaggttta taatttcttt ttaacatttc atcatgtaca atttttgtat
     2581 aaaatttaaa ttcatctata ctataattca atactttatt cactaataga tggtttggag
     2641 tacctttttt atctatagaa ccttttatag caagtagctc tctccattga cttactaatt
     2701 gatttttagg taatacatca attaaatcct tatgccataa tctcataatt taactcctta
     2761 ctataaaata ttttataaat tcaataacca atctatctta caattttaca tgttcaataa
     2821 ttttatctgt aaaaactata ccatttaaat ggtctatctc atgacaaaga cattttgcca
     2881 taaaacctga acctttaatt tctattttct caccgtattc gtttagagct tgcactgtaa
     2941 ctttttttgg tctttttaat ttaccccata cttctggaaa acttaaacat ccttcaacaa
     3001 caatttgttc cccttcttgt ttaataattt caggattaac aagcttaata agtccttctc
     3061 ctaaatctat aacaactaat cgctttaata ctcctacttg gcatgcagct aatcctcctc
     3121 catttggagt attgtacatg gtctctgcca tgtcatttaa tatttctctt atcttattat
     3181 ctacgttttc tacatactta ctcttttttc ttaaaatttc atcatcaaat gttcttatct
     3241 ttcttatagc cataatattc tcccctactt atctgtgctc ttgattttct ttcactttct
     3301 tcaaataaaa atacactttc aattggttca ttaaaaattc ttgaaatatc atatgccaac
     3361 catattgaag gttcattttt accagtttct atagcattta tagcctgtct agatgttcca
     3421 actaatcttc ctagttgttc ttgtgtcaat cctaattttt ctcttaatat ctttatttta
     3481 tttttcatat ttgctccatt catgtaaggt taactttaca tcttaaatgt aacatttttc
     3541 aacattactg tcaagtcaac cttacattta tatattaaat tttcttattt aactattttt
     3601 ttactaaact ttttaaataa taataaaatc catattaatg atataacaat tgaaataaac
     3661 atatagatat aattatcttc cttaaatgcc ttcgatatcc aaaaagttgg taagaagaac
     3721 agaatatatt gtgctttatt taaaataaaa aatggtgcta aaatacccat cataaaaaca
     3781 cctgagaatt tcgtaattac cattccttct agtttatttg atgataaaga aatcactagt
     3841 attgatataa taactccttg taataaaact aataaagaaa ttgataagtt taacaagaaa
     3901 gaaattttag ttagagaaaa aaagataagt agaatcatag taataataaa tgctaaggta
     3961 cctggtattc caaatcgtga aataagataa ccagttttac caagcggagt tactattaaa
     4021 tatcttgaaa tgctgtcatc aatttctccc aatatgacca ttgtagaagc aaaaaagaac
     4081 ataaaaggtg taataaaagc taaaagtaaa tcaaacatca agaaatacgg ctctaaattt
     4141 aattgataat aaaacttgtt aagtgatata ttctgtatta atggtattcc aaattttata
     4201 aatactccac ataatattgg agaaacacat actataaaaa gcatagcatc tctcttaatt
     4261 tgttcaaact cttgtttaaa cgcatttata atctttatca tagttttact ccacctacct
     4321 ttttccaact gctacaaata aaataataag ttataaaata aattattatt ataattgata
     4381 ttaaaatatt aatcattata aaatttttat ctccactaat aagagaaata catatattaa
     4441 aagggtacaa atttgcatat gattttgtgt caagaagtac attgagtatt ggtggaataa
     4501 agcatataat ctcaatagga atacttaaaa caataaactg attcaaacta gatgctttac
     4561 ttgcaagtat caatccaagc aaagaaaata taatagaaga aaaaaaggta cctatagttg
     4621 atattataat attatctaaa tttaatgtaa tagcaataaa catagctaca atactagata
     4681 ttactcctaa tgaaattacc tttgataaaa tatactcaga tacttttact ggagatacag
     4741 ctaaggaatt taaaaccctt tgacttttct caagaagtac aatagctccc ataaaaaaca
     4801 atcccattgc tgctgggtca gaataaatca taattatagc tattttttct ctcataaaag
     4861 ttggaaatac attgataata caaatatata taatacttaa aaaaagatat aaaaaataaa
     4921 aaccatactt aaattgaaac tttatatctc ctaaaataag attttttagc ctcattgtaa
     4981 attcctccct gttatctcaa taaaaatatc atttaatgtt ggttcactac tatgtattga
     5041 taaaagttta tttttcttaa taagcatatt caaattttta tcattagcag tattgttcag
     5101 aaaacattca gaagtttttt ctccattatc aaagtaagta tatctaactt taatagctcc
     5161 ttttgacata ataagattat gtggtgtatc taaagcagat attttaccat ttacaatgaa
     5221 cgctactctg tcacaaagct ccgttgcatc taacatgtta tgagttgtta aaataatagt
     5281 tttaccttta gatttttctg ataaaattat atctttcata accttgctat ttgacgggtc
     5341 aagaccactt gttggttcat ctaaaaataa aatatctgga ttatgtaata aagcttttat
     5401 aaaatttaat cgagatttca taccttttga atactcagat acccttttat ttgcttcatt
     5461 ttctagtcct actgatttta ataactcatc aatagatagt agttttttag aatatagaga
     5521 cccaaaatat tttaaatttt ctattgcagt caacttttca tataaactag gaaattcaaa
     5581 atcaactcct atattttcat aaaatttatt tgagtgtctt ttactctcta ctccatttac
     5641 aatgacactt cctccataat tagttatcat tccaattaga atcttttgta atgtagattt
     5701 tcctgcacct gatggtccta aaaatcctag tatttcccct ttttctactt caaagttaat
     5761 attttgtaaa aaatttctat ctgtataact aaatgataaa tcatcaactt taatcattca
     5821 atatcaccct ttcaaatttt tgactatttt gactatatgg tcaataaaat ataatttttt
     5881 tgtttgttaa taatttttaa taaaagttat tagcaaacaa aatttaaatt ctattccata
     5941 agttgtataa ctaatccgtt tatcattaat cttaaagcat cttcaaaaca attacttcca
     6001 acctcgcgct tatatagcat ggtaagaaaa actactctaa atgctccact aaagctttct
     6061 atgtctttat tttttgcatt tggtatgcaa gatataatct ctccaatttc aaagtcatca
     6121 tgtctaaagt gttcttcaat tatagaatct ggtaactttc tcaccaatat ctcaaattct
     6181 ccatttatca tgagatttaa aattgaagta ctatcaacca tcctatagaa tttcataaat
     6241 atatctgtca attgttcaca tgtaatactt tcatctactc tatttatctc atataagatt
     6301 tctttttgta tagagtcatg tatatctctc aatacctcaa aaaagagcaa ttcttttgat
     6361 tgaaaaaata gataaaaagt tccttttggt atttttaccc gttttacaag ctcatcaaca
     6421 gtagtttttc taataccata ttgctccata cacaatcttg cttcttcttt aagtcttttt
     6481 attatatact ctctttcttt atcagaatac gatttaggca tctttttcct ccagtttgac
     6541 taaaataatt tatatagtca atataatact ttttctatct aatgtcaata gtattattag
     6601 tatatttaaa aataagctta aaaactttcg aaaattctga attttatata gcatttttta
     6661 ttatctagtg ttataattaa gatataaata atatattatt ctaattttca tatctatttg
     6721 cttatttttt aacttagttt ctaatagaag tactcatatt tatcttttta aaagtttcaa
     6781 ctttaataaa aaataaagga gttgatttta tggctactac tacaaaaaac cgtttttacg
     6841 attatgttca agaaaactta aataaatatg ttgaagtaca ttcttacttt agcagttgtc
     6901 ctctatatgg tgaaattata gaagcagata gcttatcaat tacactttgc tcaagatata
     6961 tagatgaatc taatccaact ttaaatgaaa cttacacatt atatttacca ttaagttcaa
     7021 taatatctat tagaacacta taaaaagtta attaaatatg ggttatttat at
//
LOCUS       DAMKPG010000143         7040 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMKPG010000143 DAMKPG010000000
VERSION     DAMKPG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.149"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..257
                     /locus_tag="PC621_003407"
     CDS             <1..257
                     /locus_tag="PC621_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110519.1"
                     /translation="NTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYILENK
                     KHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(686..1063)
                     /locus_tag="PC621_003408"
     CDS             complement(686..1063)
                     /locus_tag="PC621_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4110520.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            1404..2516
                     /locus_tag="PC621_003409"
     CDS             1404..2516
                     /locus_tag="PC621_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF4110521.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(2724..3200)
                     /locus_tag="PC621_003410"
     CDS             complement(2724..3200)
                     /locus_tag="PC621_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110522.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(3399..5024)
                     /locus_tag="PC621_003411"
     CDS             complement(3399..5024)
                     /locus_tag="PC621_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4110523.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(5362..6381)
                     /locus_tag="PC621_003412"
     CDS             complement(5362..6381)
                     /locus_tag="PC621_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF4110524.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(6378..6947)
                     /gene="mocA"
                     /locus_tag="PC621_003413"
     CDS             complement(6378..6947)
                     /gene="mocA"
                     /locus_tag="PC621_003413"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF4110525.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         2500 a         1144 c          694 g         2702 t
ORIGIN      
        1 aaaatacaaa acaaattaca gtagaagtta atgaaatttt attacaagaa atatctaaga
       61 taactaatga cgaaaataat attttgacag atttgataaa tgaatcaatt gtaaattata
      121 ttcttgaaaa caaaaaacat aacattgaaa ataaagattt agaagaagtg attaaatttt
      181 acaaaaaaga aaatactgaa aaacctaaaa gtgcagcaag agctatagct gaagcaaaaa
      241 ctcataccct gtattaatca aataaaaaag ttcctaaaaa ctaggaactt ttttatatta
      301 ataattatta atatatctca atatttatct caaatttatc aatataagca tgtttaatac
      361 aatctattgt atacttatct taccctagaa ataatcaaaa caaaaattat gccctttata
      421 tctaacaatt aatttacatg attaatatac aaaaattctg acttttaaat tcgtatacaa
      481 tatcaaacta aaataatact aatgacaaac ccaatataaa ttaaattcaa acaggtttga
      541 ttttacatca aacttattgt attaatatta aataacaaga caaaatattt ataaaaatta
      601 caaattttac ataaaacttt atattttatt catttaacta attattgtaa aaactattta
      661 ttttatgata attaactaca ccaaattatt catctttttt aatatcttta acccattttt
      721 caacagagtt caattcttct tcatttaccc cctttttgaa caaggatttc ataaaactct
      781 caatagaact tccatgcata ttatctaaaa aagtttttgt ttcaaaatgt aaatactctt
      841 cttctccaat aagaactgta taatgtgtat gtctatcaat ctttcttgca cttaagaatt
      901 gcttatttac taatcttgat aaaagtgtta gtgttgtagt ttgcttccaa ttatatagtt
      961 tttccatagc ttcaatgaca tcttttgatg ctactgtaaa atctgaactc caaataaatt
     1021 tcatgacttt taattctgct ggtggtatct tagtaatagt catatgattt tacactcctc
     1081 tcaaacttct acataattaa atactatatt aatagacttt tcgagtcaaa ggttttattt
     1141 aaaatatctc atccaaattt attaatacaa aaaatttata tttttattca aaattaaaca
     1201 cacaatctat taatataaag ataaatatct attctataag tataacagat tatcaaatta
     1261 aaattctaat atttgacata atatatacat tttttaaata aatatataca atgtattaaa
     1321 atgtatgcaa ctctatttca aaattatttt gtgatatata tatgttataa taaatgtata
     1381 aaattttgat tggtgggtta tatatgaatg catttattag aaaaagaaat aaaaactatg
     1441 ttgtttacct ggaatttgaa gatgaaaaga caggtaaaag aaaacaaaaa aatatgggta
     1501 cttttgataa aaaaagggaa gcaagtaaaa gactaaatga ggtaaaagaa agcttatata
     1561 atgatggttt tttagtacca agcgaaatta cagtgtcaga atttctatta gattttctaa
     1621 ataaatatag tgaaaatata tctttagcta catataataa ttatgttagt atttgtaaaa
     1681 attacattaa tccatctatt ggaaaatata aaatacaaga cttacatccg gttcacatac
     1741 aaaactacat agataaacta tcttataaac taaatcctca atcaattaag attcatataa
     1801 atatattgaa acttgcaata aaaagagctt atagactcaa attaataaaa gaaaatgtta
     1861 tggatagtat agaggctcca agatataaaa aatttaaaaa tgagatttat gatagagaac
     1921 aaatgataaa attattagat ttagcaaaaa atacagaaat ggaattacct attaatttgg
     1981 ctattggatt aggtcttaga atttcagaag ttttaggtct aacttgggat aatattgatt
     2041 ttgaagaaaa tacaataaca gtaaataaga taacttcccg aattaatggt tctgttattc
     2101 tcaaagagcc aaagacagaa agctctgtta gaaaaatatc ttctcccaaa gaattaatgt
     2161 ctttattaaa agaatataaa ataaaacaga ataaaaattt attaaaatct tccattagaa
     2221 ataataataa tttgttgttt ttcaataaga aatgtgaacc tattgctgaa gatgttatga
     2281 gtaaaaaatt taaaagattt ttagaaaaga atgaattacc tcatattaga ttccatgatt
     2341 taagacactc acatgttact ttacttataa attctaaagt acctataaag gttatatccg
     2401 agagagtagg acactcaaat ataagtacta ctctaagcgt ttattctcat gtacttaaag
     2461 aaatggataa agaggcttct gacaaaattt ctgaaagttt atttaatgct aattaatatt
     2521 aatttatctt agctttaaat tagcttagct tattgagata gattcattaa tattaaatgt
     2581 atctatctca aattaataac tacttaaata atctgtttta ttttaattta tcgcctcagt
     2641 tattatactt ttttatgaaa aattatactt tacaataaaa aagatatttc cttatagaaa
     2701 gtatcttttt aaatactatt ttattatata cttgctaaca ttggaatatc taacatcttc
     2761 ttcattacat catctacttt ttttgcttta tattcattgt atccaattaa atatcctttt
     2821 tcatatgaac tttttaaaat ctctttttca tataaattat cagatttggc ttctgtgaca
     2881 cttaaattat aaagcccttt acacccatct tcataacctt tattataccc tttttctgca
     2941 aaatagtttt tatgattttt ttcgttatgt actgaagata ttgttactcc agaattttct
     3001 tttgtactag cataaggaca aaaaccttta tttggttgat gtgctgaatg tcctccacaa
     3061 taataacgtg ttaactctga tttattttta ttatgatgac ctcctgatga attatgccct
     3121 cctgagtgag catatgaaaa attccctaca ctaatcatag ctaaactcaa tactacagct
     3181 actaaccttt tcttaaccat aatctattcc ccccaaaatg ttatatattt ttattatatc
     3241 atctactata ttatatttaa attcttaata tacaaaatat tactcgattc tgctaataaa
     3301 catatttaaa caacataaaa aacctcctaa tatttgatta aaaatttaca cactcatgca
     3361 tagcatgtat caaatattaa aaggttttat actaataatt atttatcttt tccatacatt
     3421 ttagctaatt ggtcttgtat ctctttactt tctaaatact catcaaagtc catttttcta
     3481 tccattattc catttggagt tatctctata attctatttg ctatagtaga tataaattca
     3541 tggtcatgag aagtaaataa aagcactcct gggaactttt caagtccttt atttacagaa
     3601 gttatacttt ctaggtctaa gtggttagta gggtcatcta atacaagtac atttgcattt
     3661 gaaagcatta gcttactaag catacatcta accttttctc ctccagatat aacttgagct
     3721 tcttttaatg cttcttcacc tgagaataac attctaccta aaaatcctct tataaaactt
     3781 tcactttttt cttcagagaa ctgtctaagc cagtcaacca aagaatattc tactccgtca
     3841 aaaaacttat tatgattttt tggaagataa tcttgagaag tagttactcc ccatttatac
     3901 tctccactgt ctggttcaag ttctcccata actatgttga aaagtgcagt agtagctatt
     3961 tcatctccca taaatactac tttatcatct ctatccaatc taaatgacac attatcaaga
     4021 accttaaccc catctatagt tttagttaaa ttgtgtactt ctaaaacttc attcccaatt
     4081 tctcttgcag gtgtaaatcc tacatatgga tatcttcttc ttgatggttg tatatcttca
     4141 acttctaatt tctctaactg tttctttctt gatgttgctt gtttagcctt tgaagcatta
     4201 gaactaaatc tagctataaa ttcctttaat tgagctatct tttcttcagt ctttttattt
     4261 tggtccttag ccagttgtaa agccaattga cttgactcat accagaaatc atagtttcca
     4321 acgtacattt gtattttacc aaaatctacg tcaactatat tagtacatat ttgatttagg
     4381 aaatgtctat catgtgatac tattataact atagactctt ctaaatccat tataaaatta
     4441 tttaaccagt ttattgattt aaaatctaag tggtttgtag gttcgtccat taatagtatt
     4501 tcaggcttac caaaaagtga ttgtgctaac aataccttaa ccttttcccc accaactagt
     4561 tccttcattt gtttgtaatg catatctttc gttatgccaa gacccataag tattttctca
     4621 gcattagttt ctgcatccca tccgtcaagc tctgcaaact caccttcaag ttcggcagct
     4681 tttatcccat cttcttcatt aaagtcttcc ttcatataca atgcatcttt ttctttcatt
     4741 atgttccaaa gtctctcatg acctcttata acaacattta aaacagtttc ttcttcatat
     4801 tcaaagtggt cctgttttaa aactgacatt ctttctttat cagttataga tatactacct
     4861 gtattaggct ctatttctcc tgataatatt tttaaaaaag tagattttcc cgcaccatta
     4921 gctcctatta ttccatagca attaccttta gtgaatttta agttaacatc tttaaatagt
     4981 tccttatcac caaatctaag tccaacacct gtaacttgta acattaatat attttccttt
     5041 ctttttgctc ataatttatt ttatcataaa actttgataa catttttatt atatattatc
     5101 acgtaaatag tatatttttt atactaaatt tgtgaaatat ttattaaaat aatactaaaa
     5161 attgtaaatt tataaaaatt aacaatctct tatattatac ttataaagaa atcaaattgc
     5221 aactttattc attcttttac acattgaaaa taagttatta taatttctaa ataaatatgc
     5281 ttttcttaaa acatcattct agctgttttg tttcatattt aaaaagagta cttaatttgt
     5341 aaatactctt ttttaataac tttatatttt agcaaaatta catgcaggat aagtacatac
     5401 atcacagttc atacaaaggc caccatgacc atatctagct atatcatact tatcaagctt
     5461 ttcatcaatt aaaaccctag ataaaactat atcaaatact gtagttttat gatacattgc
     5521 gcatcctggt atacccataa ttggaacttc tccataataa gcaagtaaaa acatagctcc
     5581 tggtaatatt ggtgaaccat atgtcactaa atcagctcca gtttctttta tagctgttgg
     5641 cgtaacatca tctgggtcta cactcatccc gccagtacaa cagataagtt ctgctccttg
     5701 acttataaat tcttttattg cactctttat aatttcttta tcatctgggc atatagtctg
     5761 tcctataact tcacaaccaa agccttttac tttttcttct attactggtc caaatttatc
     5821 aacaattctg ctataaaaca cttcatttcc tgtagtaaca attcctactt tcttaggttt
     5881 aaaaggtact acatttacta tttttctatt accaactact ttttttgcat cttctaattt
     5941 tttctcatct ataaccaaag gtatcactct tgttccagct acttttaaac ctttatttac
     6001 tataaaatta ttatgaagag tagccatcat tatttctcca acacaattca aatcaaaaag
     6061 tgcatcaaca tctatcttta atagaccatc acatgctgct ataaaatcta tttttccttc
     6121 ttttatttca ctaaaatcta aattttctcc agcagttagt tcttttaaaa acaatgcgcc
     6181 ttcattttca tgaaccattc cttctgactt ttcccataca tacaaattat ctttaccaag
     6241 agataataac actggaatat cttcttcttt tactatgtga ccctttttaa attttcttcc
     6301 tttaaattct ccaggtatta tttgagttat gtcgtgacat aaaacttgtc caacagcatc
     6361 tatagttttt attagcttca taatttaccc ccgattctct catagtccat ttttgtatcg
     6421 atatcaaata acatttcttt aggaacttct acatacttta tttttgatga attttttaaa
     6481 atggttttac ctttttcatc ttctttaagt gccaacaatt cttctttctt actatatggg
     6541 aatatgactg gatttccaca catatctttg tatcttggaa ttactataaa atctctatct
     6601 aatttaaaag tatttataat tttatctata tataagttat caataaatgg ctggtctcct
     6661 acaaagaaca tataaccatc acattttgag ctatttaaaa ctcctaattt tatagattca
     6721 ctttgaccta tatttgcatt tttattatct atatattcaa aaccatattt attacacaat
     6781 aacaaaacct cttcatattg acttactact acaacttgat gaaaatcaat cttgctaact
     6841 tctctaaata catgttctat tatagaacag cctttataat ttaataaaag tttatttgct
     6901 cccattcttc tggcaaagcc tgatgccatt atcactgcat ttatcatata tatcatcctt
     6961 ggtatttata atatattgat agttctatga tttttataat atctctattg atattatcta
     7021 tatatataat atcatataaa
//
LOCUS       DAMKPG010000144         6721 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMKPG010000144 DAMKPG010000000
VERSION     DAMKPG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.150"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(41..649)
                     /locus_tag="PC621_003414"
     CDS             complement(41..649)
                     /locus_tag="PC621_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF4110526.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(758..3496)
                     /locus_tag="PC621_003415"
     CDS             complement(758..3496)
                     /locus_tag="PC621_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF4110527.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3644..4435)
                     /locus_tag="PC621_003416"
     CDS             complement(3644..4435)
                     /locus_tag="PC621_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4110528.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4471..5127)
                     /locus_tag="PC621_003417"
     CDS             complement(4471..5127)
                     /locus_tag="PC621_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110529.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5120..6085)
                     /locus_tag="PC621_003418"
     CDS             complement(5120..6085)
                     /locus_tag="PC621_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF4110530.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(6191..6297)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         2134 a         1139 c          615 g         2833 t
ORIGIN      
        1 aataccttcc tatagataca tatcttacct atataaaaac ttattctata taaaaacgcc
       61 ataaaaaatc ttttgcttct tctgcataat ctatatttat acgtttacta gtttttatat
      121 ctaactcatc tttactaaat actttttttc ctttatcatc ataataaaaa tcacttatat
      181 ataattcttc acccattata ctcttactat ttagacttct atctattttt aaagctttgc
      241 aaagttttcc tggtccgtta gtaatatttt ttacttgata tttactcaac tcagtgtaag
      301 ctttgttgta tctattcata gatatctcat ctaaagaagt tataggctct acccctctta
      361 ttaatacaca ctctggcaca ttttctatat ttgcagataa atttaagcaa ttatacatac
      421 cataaattaa ataaacatat atatgtccac catctaaata taatggctta gttctatctg
      481 tctttttatt gccatataca tgtgctcctt tatcatttac acccatgtat gcttctgtct
      541 ctatgatttt ggtaacaatt actttatttt catatttcct tattagatat ttacctaata
      601 ttgatttggc taaatcaatt ccattttttc taaaaaaatc cttttccaaa atttcacctc
      661 ataaaattta tttaaaacgc atcaaataca actaaagtaa taaataaaat taactcttat
      721 taatgtgata tatataaaca ataaatttag ccatttatta tttatatata tcaaaaaatt
      781 ctatctcaaa cttttcaata ttatcattgt aatttgaatt atcatatact tcttcaacaa
      841 cctctttaat tgggaatata acttttacat tagttccttc tcctacctta ctatctatgt
      901 aaatcttccc attttgaatt tcaatcaatg attttgtaag gcataaacct ataccacttc
      961 caaattcatt tgatatacca ctgttttcaa tttgtttaaa tcgattaaat atatcattta
     1021 atttttcttt gtctatgccc actccagtat cttttataat tatctctact gtttcgtctt
     1081 ttttatatat acctacaagg atttctcctc cttctaaagt aaattttata gcatttgata
     1141 atatatttag tattattctt tctatagatt ctgggtcaag ccctaccata acagtttctt
     1201 catccgtatc aaaaattaaa tctattcctt tagacttagc ataactacta atagatgtaa
     1261 ctattctttc tgttatgtca ataatatcaa aattaactgc tttaatctta tatatgtctg
     1321 acttaacttg agaaatattt attatattat ctattaatct caataatcta aacatatttt
     1381 gtttagttat ttttatatgt tcttttagta tatcctgaaa atcaattatt tttccttttt
     1441 tataaaggtt ttcaataagt tgaactgaac tatatatatt atttaaaggt gtcttaagtt
     1501 catgagatat attagcataa aactccatct tcatttggtc atactgtctt tgttttatta
     1561 atgctttttt aagtctttca gactctcttt gcacacttaa atctttaata attctcactc
     1621 tacttctttt ctgtccaaag tataatgttc ttgatatcat ttttacttct ttaatgtgat
     1681 tattttcatt tactatatgg atactcttct ctctttctcc tatatttaaa ttgtttatgt
     1741 atttttttac taatatattt ctatattttt gtgttgtaac tcctaataca tcactagaaa
     1801 ttccgaacaa ctctctaaag gctctatttg tatatagtac tcttgatgta cttaaatcct
     1861 caagaaaaat agcctctggt ataacatcaa gtaatttttc atatattatt ttttgttcat
     1921 ttactgttct aagaatattt atctctttag ttatatctct aaatgtaagc aatttaaaga
     1981 gtttattatc tgaccaatag tctccaactc taacttcttc aacatgttct tcaccttctg
     2041 agtcagacat cacaaaatta actatacagt ttgctgtgtc attgtaactt actatttcag
     2101 ctttcttttc ttgtgttaga tttaatcttt taaatttcct attgtcaaag aaatctatgt
     2161 taaaagtttc aaaatattgt tctaatgtca tatcgactaa atcacttgaa acacatttaa
     2221 aactcttttc atatttttta tttatactta ttaccttatt cttctttatg ccaccttcag
     2281 cacttcttac aagtatagga gatggaatat ttagtataat gttttcatag tctttattta
     2341 tatttcttat ttcttcatct attttgattt catttgtaat atcatttaaa cttattaagt
     2401 aattgctttc atctttatta ttacttaaac ttaacaaata aattttatca tttaactcta
     2461 ccagtttatc aattatactt atatcgttat caaatttctt caatttttca gcaacttttt
     2521 catcttcctt tataaaagtt aatattttat aataattatt catatccttt gaaaattcat
     2581 ctttaaattt attatttgtg taacttatat taaaatcata gtctgttact atccatacat
     2641 ttggtattgt atccagagct tgtctaagta attcttttct ctggtctatg tagtttttta
     2701 atgttctttg tataatcata tccttttcta ttgctaaatt tatctttcct aattgctctg
     2761 actttttact taatttttta ttgattgaat caagttcata atatggatac actactctat
     2821 tattaagact gtatacatat acataataag tatggttaca taatagtaaa tatattactc
     2881 ctattaatct atctgaatct actatattaa tcttagaaat tgctacaaaa catataatag
     2941 ttgtaaaaaa aacaaaatat gttaaatctt ttattaactt atagtgctca atgttactat
     3001 caagtttatt caacaaatat agtaaattaa acatagatat acaaatatta attaatataa
     3061 taatcatatt ccagttaata tcactaaaaa tatttcttat aaagtaatct aatatcacaa
     3121 aagcactata aaaaattagt attgggctta ttcttagttt ttttacttta gaaatggtta
     3181 agtatataaa agtagacata ttaattatgc ttaagttaag caatacaatt tggaattttt
     3241 gaatttttaa atttactact cctaaaatac ttacaaaaac taatacaaga caccctactt
     3301 tcaatatatt tataaattca tctgttttaa agctacttat ataaactata gaatatccaa
     3361 atatccaaag taaaattatt ctatacatat ttatagaaac tgtatacaaa tttatattta
     3421 aaagtgcaat aaccaaaaat aacccattag ctagtatata gaccttattc ttacttaaag
     3481 ttcctttaga ttccatattc cccaccactt cttcattttt atatagtttt acctataaat
     3541 atgttaccac aattcttcca aaaaatttgt cactttctag ttataaaaaa aaatgagtaa
     3601 atatttttaa ttttactcat tttgtagatt ttgtatattc agtttaaaaa gctggaacta
     3661 tacttccttt atattcatct tgtataaatt ttttaacttc tttactattc atagcctctg
     3721 ataaagcctt aatcttatca ctatctttat tattttctct acaagctatt atatttacat
     3781 atggagaatc acttgattct attactattg catccttagt aggatttaag ttagctgtta
     3841 aagcataatt agaatttatt actgctccgt caacatcttt taatactgtt ggtaattgct
     3901 ctgcattcat ttcttttatt actatatttt taggattttt agttatatct tttttagtta
     3961 taagctctcc atcttttact tctatcaatt tattttttgc taataattta agtgctctag
     4021 ctccattagt tgcatcattt ggcacagcta taacagctcc atcttttaat tcatctaatg
     4081 acttaacttt ttctgaatag aatcccattg gctctatatg aactttagat gtataagtaa
     4141 gtttatatcc tttctccttc acagtttcct ctaaaaatgg aatatgttga aagaagttag
     4201 catccaaaga cccttcatct aaagctgtat ttggaagtac ataatcatca aatattttta
     4261 cttctaaatc atatcctttt tcttttaaca aaggttttgc aacttccaat atctttgcat
     4321 gtggatttga tgaagctcct actagtattt ttttatcctc tttgtttgaa caacctactg
     4381 ctgatattgc tattgctgac accaatgcta ctgataataa tttctttaat ttcattttta
     4441 ctccccctct ttaaaatgtt tataattaat ttattttagt ttcttataag ccagatttcc
     4501 tactccctgg attatctgaa ctaatattat aagtgctatt acagtatata ccattactgc
     4561 tgtatcaaat ctttgatatc cataaataag tgctatatta cctaatcctc cagctcctac
     4621 tgcacctgcc atagctgtat aaccaagtat agatattatt gataatgtaa ttccagatac
     4681 tattgaaggc atagcttcct ttatcataac tttaaatact atttgtcttt ttgtagctcc
     4741 aaaagatttt gctgcttcta tcaatccttt atcaacttca tttaatgagc tttctataat
     4801 tctagcgata aacggtgctg ccccaatagt tattggcact attgcagcag tttcccctat
     4861 acttgttcca acgattaatc tagttattgg aatcattgca actataagta tcataaatgg
     4921 gaaacttcta aatatattta ctataaatcc taatgcacta tttattgtgc tatttggttt
     4981 taatccatct ggttttgtaa ctactaatat gattgctaat ataaatccta atattgttgc
     5041 cactatagtt ggtactataa ccatatacaa agtttgtaac aatgcatttg gaaatagtgt
     5101 tgttaaaaag tcaattaaac tattcattta tcatttcctc ccatcttatg cccttactag
     5161 ttagatattg ttttacgtgc tctccacttt tatctgaaat gtttataata agtgaaccta
     5221 aaatatcatc tttaaattgt tctaatttcc caaatattat agacacatct atatttagtt
     5281 ctcttgccat agtcgtaata atagcttcat tagaaatatc tttagggaat agtatcttta
     5341 tatttgttcc ttttggcaat attatacttt cttcacctat taattttcta agtcctttag
     5401 tatttcttaa gaatatttct tctgtatctc ctacctctaa tacttctcca ttttccatta
     5461 ttgctactct tccacaaatc tgctttataa cttccatttg atgagttaca actattattg
     5521 ttattcccaa ttttttattt atatcttcaa gcaatgaaag aattgacttt gtagtatttg
     5581 ggtctaatgc tgaagttgct tcatcacaaa gaagtacttg tgggtttagt gctaaagctc
     5641 tagcaattgc aaccctttgt ttttgtccgc cacttagatt tctgggttta tcatttttct
     5701 tttcagatat tccaactact tctaataact ctaaaactct ttttttaatt tctgcctttg
     5761 agtatccaaa acattctaat ggcaatgcta cattatcaaa tacagttttt ctttcaagca
     5821 atgaaaagtg ttgaaaaatc attcctaact cttttcttaa atctctcatt tgcttttcat
     5881 tcaatgactt tacttcttta tcagaaacaa gtacactgcc ttcttggtat tcttctaatc
     5941 cgtttataca tctcagtagt gttgatttcc ctgcaccact atgacctatt attccaaaaa
     6001 tttctccaga ttctatttca atacttacat ctttaagcac ttgaatcttt ccataatact
     6061 tattaacatt ttttatgcta atcataaaat ttcctcccat atattctgct cagtttttat
     6121 gtttatcctc tcttatgtat tacaacaaaa taaaagcccg agtataaact caggcttaga
     6181 aagctattta tactcatcta tcaactatat tagttgcagg atttagcacc aatgtatata
     6241 aaatatactg gttgccgggt ttcaaagggc cagtccctcc accactcttg ataagacgtt
     6301 atttaattca attctattat catttgttgc tatgttatat atgatatatt cattaatagt
     6361 atttgtcaat aaatttattt aaatttttta ttttttatta tcgttaatta actattttta
     6421 attagttttc tagctcctaa cactttatat tttaatgtct atataactat agaacatcat
     6481 tataagaaat aaattaaata acatcaatct atctaaatca tctgtaaagt aaattaagaa
     6541 aattaacatt ttatccaaac tctatcctac tattaaataa aataatttaa atacaatgat
     6601 gattatatag ctctaatact ttagactttc tgaacctccc atgactaaag tcacagggtt
     6661 cctgcttcat agaattttgc atactaaaat atgtcttact aattctccac aggctatccc
     6721 c
//
LOCUS       DAMKPG010000145         6709 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMKPG010000145 DAMKPG010000000
VERSION     DAMKPG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.151"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..1674)
                     /locus_tag="PC621_003419"
     CDS             complement(<1..1674)
                     /locus_tag="PC621_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110531.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAAYKRYTDILELNNDGQLLAGKEVNLMYGNIEFNEISFL
                     YNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIGLLKPSEGTIFIDDYNLLE
                     MNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDIIEVLEKVGLKNLYLKLEAG
                     LDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATSALDNITEEKVMLNVMEFL
                     KDKTTIMIAHRFNSIKNVENIIVLKEGSIIGSGSFKKLLDGNLYFRELYY"
     gene            complement(2597..3178)
                     /locus_tag="PC621_003420"
     CDS             complement(2597..3178)
                     /locus_tag="PC621_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4110532.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(3171..3401)
                     /locus_tag="PC621_003421"
     CDS             complement(3171..3401)
                     /locus_tag="PC621_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4110533.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            complement(4726..5001)
                     /locus_tag="PC621_003422"
     CDS             complement(4726..5001)
                     /locus_tag="PC621_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF4110534.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            complement(5077..6378)
                     /locus_tag="PC621_003423"
     CDS             complement(5077..6378)
                     /locus_tag="PC621_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4110535.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
BASE COUNT         2301 a         1231 c          547 g         2630 t
ORIGIN      
        1 atagtataat tctctaaaat ataaattacc atctaatagt tttttaaagc taccactacc
       61 tattatactc ccctctttaa gaacaattat attttcaaca ttcttaattg agttaaatct
      121 gtgagcaatc ataatagttg ttttatcttt caaaaattcc ataacattta gcataacttt
      181 ttcctctgta atattgtcta aagctgatgt agcttcatct aatataatta tcttagcatc
      241 tgaaaaccaa agccttgcta atgctagcct ctgcttttct ccacctgata atgtagttcc
      301 tttttcacct aatttagtat ctaaaccagc ttctaatttt aagtataaat tttttaaacc
      361 tactttttct aagacttcaa taatatcatt actttttata gtttttccaa atacaatatt
      421 ctcacgtaaa gttccatcaa atataggtga atcctgtgac acatatgtaa cataattata
      481 gtaactattt agattcatct caagtagatt ataatcatct ataaatatag ttccttcgct
      541 gggctttaat aatccaatta atagtttaat taaagttgat ttaccagaac cactctctcc
      601 aacaatagct actttttctc cacttttaat acttaaacta agtttattaa atatctctcg
      661 cttattatta tacaagaagc taatctcatt aaattcaata tttccataca ttaaattaac
      721 ttctttacct gctaacagtt gaccatcatt attaagttcc aaaatatctg tatagcgttt
      781 atatgctgct ttatctagtt taaattgaat atacaataca ttaaaaatag ctataggagt
      841 atatgcatta tctagtaaag ttatcaatgc aattatagaa cctatactta ttgttttagt
      901 tttccaacta taaactatta ttaacatttt tatgaacgtc actattaatg aaaaaatcgt
      961 gaagaaagct tcatgaatca gtgtcatttt aaccctagaa tcaataattt cttttttcgc
     1021 aaattttgac tgacaaattt catactcaaa tcttttgttt acccgaaata caaccatttc
     1081 tacaaatcca cgaactaaaa aatgattcat cttctcttca ttaattaata tatgctcttt
     1141 gatttgatat agagctttta ttagtaaatt agtaataata aaaactataa aatatccaat
     1201 taatatggta aacataactt ttttatcaat tctataaata aaaaccatac taaataaaat
     1261 tgatggaatt atttttctaa caacggctaa taaaaaatta aaaaatatat ttctgccagc
     1321 attagctcca ttttcaattt tttgaattaa ttttccagtg cctaacatct gatattctaa
     1381 gtaatctatt ttactcattt tttctaaagc ctttaatttc aaatctaaaa aaaaactatg
     1441 ttctagtctt ctacctggat attcatcaat atatcttaaa agacataaag taactaatac
     1501 agttccatag ataaaaatta tgtgaaaatt tatagtactg ttattaaatc tatctattag
     1561 gctctgataa taattagcac taaaactatt taaaagttct attcctatac ctagtgctaa
     1621 atatataaaa actaataacc tctctttttt tgctatttct attaaatatt tcataactgt
     1681 acacctatcc ttatttattt tactttatta acttaaaata attttataga taaaatgttt
     1741 ctttatttgt ttaaatttag tgtgattatt aatcatttca agttaacaaa acctaaagtt
     1801 tttagcagta cagttctatt tgttagcaaa ttattatttt actcagtaca ttatgctaac
     1861 atataatcta actgtactga gtaaatatct cgtttcatca tataaaatct cctttttata
     1921 gtttaatata tatagttaaa tttactatat aatctgaata tttatttttc tatcaccttt
     1981 accagaaata aaatcaatta tttattgtat aagcaaaact tcataatatt ttcaacatat
     2041 aattattata cagaactcct aagttaattt aaagttagaa tttattttta taagaaatgt
     2101 cattgaccta cctttaataa aacagccacc atttttttat gtattaacat ataatatata
     2161 gtaatgaaaa catattaatt aaaaaatatt gtataaatta gtttatctgt aacaaatcca
     2221 gtttatgttt caacaagcac gtaagacttc ttgaaattta atctgcttcc tttaaatatc
     2281 tattacttgt tttaagcata agtttacaat cttctataac atttatcatt tcttcagatt
     2341 tctctgacgt acttaaaata caagttgtta cattgttttt ctatttaatt agtaatgtat
     2401 catgtaaaaa agaagcataa atgagtttac tttatacaaa cttcatttat gctccactca
     2461 tatacctatc atagctaata gaaactatta ctcatcttga atctatttca tatttagata
     2521 tttatatctt tcttattttc ttaaatcaac tctagcttta aacattaaac cccttaaatc
     2581 ttcacaaaat ctcttcctat acagacttac tctttgcctt acatatcatc tgagtcatag
     2641 tacccatagg gcaataaaca caccatgaac gaggtttaaa caatatcata gtaatcaaac
     2701 caagaacagt tgatgtaagc ataacactat aaaaaccaaa tgcaaactgt accacccaag
     2761 gtgaaactaa agttccatga tacgcccatt gccaaggcat ttttatagtc cacaacaaag
     2821 tcactacttc tttaaaattt ctacttcctt caaacactaa ataagttgaa aaaagcatat
     2881 tggcaaacat taccataaaa aatgtcaaaa acccatatct aaaccactta cttctaataa
     2941 atttaggaat atctctattt cttgacagct tcaatttatt tccaagcaaa ttaaacagtt
     3001 gccctcttcc acaatactta ttacagtaag ctttatttcc cttaacaata gaaatagcta
     3061 gtggaacaaa gaaacaaatt agcccaatcc aagcgaaaat aatatttaca aatcccaata
     3121 ccaaatatat tgaagaaaaa atccataaat agtcatacca atgtttttta ttattcatga
     3181 cacttacctt cttcctcttt taatattgtt ataatctcaa ttacagatgc tggacatatc
     3241 ttttcacaaa tcccacatcc aacacataaa tctctattaa tttttgcact aatgccacat
     3301 ggaactgaga tagcacttct tggacatgct tttatacagc taccacaagc aacgcactca
     3361 tttgctaaaa tatttgcata cttctttttt ttattagaca taataacctc cctatattat
     3421 taatctataa gaattatatc aagatactta ctttttatct gtgacttaat cacataaaac
     3481 ctcaatatta aaaatcacac tctaaataca aaggagtttc aaataaaaaa cgatatgaag
     3541 tcattaagtt tagcttcata tcgccttttc ttttgtgtat ctatataaat atttattatc
     3601 taatatttaa tatatctata atattttctt atacagctct ataattttac acctaatcat
     3661 attctattag taacaggtta atacccatct aaaactataa ctttattcac aatcttattc
     3721 tattagccac aagttaaact catattgaag tttatcttta gttacactct tactctatta
     3781 atcacaagtt aagcccatat tgaagtttat ctctatttac actcttactc tattaatcac
     3841 aagttaagct catattgaag tttatctcta tttacactct tactctatta accacaagtt
     3901 aaactcatat tgaagtttat ctctatttac actcttactc tattaatcac aagttaagct
     3961 catattgaag tttatctcta tttacattct tactctatta accacaagtt aaacccatat
     4021 tgaagtttat ctctatttac actcttactc tattaaccac aagttaagcc catattgaag
     4081 tttatctcta tttacattct tactctatca atcataggtt aagctcatat tgaagtttat
     4141 ctttagttgc accttactct attaatcaca agttaaaccc atattgaagt ttatctttag
     4201 ttacacctta ctctattaac cacaagttaa acccacattg aagtttatct ctatttacac
     4261 tcttactcta ttaatcacag gttaaaccta ttataatctt aactttattc acaattttat
     4321 tctattagcc acaggttaaa tctattataa tcttaacttt attcacatat tacatcatta
     4381 atcgcatatc aagattttat aataagcttt aactaaacac acgattttac cttattcgta
     4441 tagtttaatc tattaaatta ttatataatc ttaagcaaac atacaactct acactattaa
     4501 ttatagcccc tccttttaaa tactcataat cttatacaca accttacctt attaatcaca
     4561 catttaactc atatatatca tcaattttat tcacaaaatc atgctgttaa tcacacttat
     4621 actcctctat aatcttaaac ttatacacaa attagccaca attttatttc aactataata
     4681 acttctattt cagaagtata tcacacttgt attctccaaa aatatctatt tttttctaac
     4741 aatatgcctg acattaataa cactcatttc aaacatcctc gctaattcca tataattttt
     4801 cccattaaac aatttaatca taagctcatt tctagcctct tttaatgccc cttccagtgt
     4861 tttaatataa aaatatgttc ccccactttg ttgacacaat tcaaggtatt ccaataaact
     4921 atacttatgt aactcctttg gaatacttac ttctatcttg tgttcttcaa gtaattctat
     4981 aattttcgtt aattccaaca tattttcacc tcatatttta taatctattt gctatttcct
     5041 gacttttaat aagcttgtta ttactacttt attgacctaa taatttagtt cttgaaaatg
     5101 taacttatcc ccaaagtaac aaaaatgctt atgcctagtc tgtccatctc tacacttcgc
     5161 aactattaaa tcaaccatct taattcccct gtattctgat gctctaactg cctcctcact
     5221 ttctccaata tctctcactg cttcttcata atcactcccc tttaattgat gtatataaac
     5281 cacattgttg ctatcgtggt agatggcttt gctatccctc ataggtctat ctccataagg
     5341 tcttgaatct ttcatttcat catttaactg acttaattgg attacaggta tattaaaatc
     5401 taaagtcata tttttaagtt ctctcgaaaa agtagctacc tccctttctc tcttatccat
     5461 gcttttctga gatgtaagga gttgcacata atccactatt aataaatctg gctttacctg
     5521 ccttatcctc tttctaattg cactaattgt atctatctta tcattaatat aaattaagtt
     5581 attttcgctt aaatcaccta tagcatttac aataagtttc caatcatttt catctatttc
     5641 tttagatttc atattccttg tacttacacc agttactctg catatatttc tcataaatac
     5701 ttgctcactg gtcatttctc tacttataaa taaagttttt ttaccttgct taaatgaatt
     5761 taacattatt tgaagtgcca aagcagtttt cccaaccccc gatttagctg ctattgtggt
     5821 tagctctcct ttaaaaagac ctcctatagt agtatcaagt aattttatac caaatttaaa
     5881 tccaatatct tttttgtttt ctaaaaaatc aagcaccttc cctgctatac tatttacatc
     5941 atcattttca taagtatcct tgtccaaaat ctcctttata ttactttcaa atttgtaaat
     6001 acaagtatcg atattttctc caaggtttag gctgtgtaaa aggttttggc agttttgatt
     6061 tatgcttctt ctagctaaat tttcttttaa aattttaata tgaccatcaa gaccaaaatt
     6121 ctgaccccaa gtaattagat tagtcacatc actcgttttt attgctagag attttctatc
     6181 tatctttgat ttcacagtgg ccacatcaat cactatgttt tcttttttaa gttctttcat
     6241 aattttaaat atctcaagac atatatcatt cataaacatg ttttcattta attcatctag
     6301 cttatagctt aatttattgt ctaaaagaat tgtgcctaaa atagacttct ctatttcaac
     6361 atcttttgat ttacccatat aaattaatct ctctttcctt ataagttttt atattttgat
     6421 ttttaaatac attatcaatt ataaatacat tatttgttat gactcactat ttgttatgac
     6481 tatattattc gtcatgatat actacaaatt attaatatac tatttattct gatacatata
     6541 atttactaca taatagcaat atcttatttg atataactta ctaccaatta ttaatacatt
     6601 gttcaatata attcattact cgatagtaat atcttatttg atataactta ctgccaatta
     6661 ttgatatatc gttcaatata attcactacc tgatagtaat atcttactt
//
LOCUS       DAMKPG010000146         6642 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMKPG010000146 DAMKPG010000000
VERSION     DAMKPG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.152"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..656)
                     /locus_tag="PC621_003424"
     CDS             complement(<1..656)
                     /locus_tag="PC621_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF4110536.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     "
     gene            complement(843..1352)
                     /locus_tag="PC621_003425"
     CDS             complement(843..1352)
                     /locus_tag="PC621_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF4110537.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1427..1840)
                     /locus_tag="PC621_003426"
     CDS             complement(1427..1840)
                     /locus_tag="PC621_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF4110538.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2277..2936
                     /locus_tag="PC621_003427"
     CDS             2277..2936
                     /locus_tag="PC621_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF4110539.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
     gene            complement(3543..3722)
                     /locus_tag="PC621_003428"
     CDS             complement(3543..3722)
                     /locus_tag="PC621_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4110540.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3947..4480)
                     /locus_tag="PC621_003429"
     CDS             complement(3947..4480)
                     /locus_tag="PC621_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF4110541.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(5298..5870)
                     /locus_tag="PC621_003430"
     CDS             complement(5298..5870)
                     /locus_tag="PC621_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF4110542.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(5932..6195)
                     /locus_tag="PC621_003431"
     CDS             complement(5932..6195)
                     /locus_tag="PC621_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF4110543.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6195..6416)
                     /locus_tag="PC621_003432"
     CDS             complement(6195..6416)
                     /locus_tag="PC621_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF4110544.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         2330 a         1137 c          814 g         2361 t
ORIGIN      
        1 tggaatacag actccattcc taggaaattg gctgaaatat agtctgctgt caaagatgct
       61 gtcatcacag aaattaaaac agatactgaa aagtttttat ggacttcttc aagtgaaaac
      121 ataacacctg ccagtggtgc atgaaaggct gctgataatc cagcacttgc cccacaggtt
      181 aacaggaatt tttcctctgt cttccctcgg tcaagagctc tagaaatacc tttacctgcc
      241 atagcaccaa gctgaatgga aggaccttcc cttcccaaag caagaccacc cataagtgat
      301 aagaatcctc caataaattt tgtgggcaac actcgccacc atacttgttt tattttccct
      361 gtcatttccc cctcaagctg agggatacca cttccagaaa tcattggctc ccagtccacc
      421 aactttgata caatccacgc aagtactgca agtactgcaa accaagctat tatttttatt
      481 gtagaacctt ctacaaattt taaaatttgg ctaagccaag taccagcctg ccctaaaagt
      541 atcctgtata aaagcacaat caaaccagcc acagccccaa ccataagtcc ttcaccaatc
      601 aaaattacct gaaagtgctc tgcccgtttg ataatatgag atgtatcctt tttcatactg
      661 ttcctccttc atccaataat tctacacaat ctgaacctcc aatgactaaa gtcatgagtg
      721 tgtacagata ttttttaatc aacaaaaata gatttttatt tactaaatat aagaaaataa
      781 ttgttcaatt tctcaataaa tgtaaattta ttcttttagg tatttattct ccacagtcgt
      841 gcttattcca gcaaaatatt tattagttct tgctttctct gtgtatagat tttacgattg
      901 tcctcaaact gtaatgctaa tttttgttta tgactatcat agagttttgc tttgtctgta
      961 aaagtattta gataatctcg aaaattgata taattattcc atgcgacact attccattca
     1021 acaacatgaa tatggtgagt acgagtattt ttctcaaaat ctcccatagc aaaaagtaat
     1081 tgttttggta catcctgacc tcgaaaaacg attccacatt gctctaacct ttcgaggtat
     1141 aatttagctt cctctaaaga agcaacacct acaacaatat caataatagg ctttgcgcta
     1201 atactgggaa tagaagtgct tccaatgtgt tgaatatcaa tggcagtctt atcccatata
     1261 cttttcaata atataatgac atcttttgca cttttatccc atatcatctg atgttccaat
     1321 aatttaacca taccacattt taaacctatc actatatacc acctcaatat aatccacctt
     1381 tatatatata ttacaatttt ttctatcttg ataaacagtg atctagctat ttcacttttt
     1441 cttgacactc gctacattcc ctatcatgaa tagatataat tcctccacat ttagaacaag
     1501 tatatctctc cttctgcttt tgcataaact tctctaaacc gtactgctga acaaatttac
     1561 tattttccat aaggcttgct tgatatctct tgttataact tttttcaaga ttttttatga
     1621 gtttacaagg aaaattgaaa cattcaaaac aataaaacaa ccctttttga cagacacaat
     1681 cctttatttt acacttacgg cagtgttctg gctttcctgt atcgctgttc aaacaaccag
     1741 cacatggctt tttgtgataa caatgcttat agcaaaccat acaattcatt ccacaaggag
     1801 caaacataat cgtatcaata ctctcttttg gcattttcat aacattttac tcctttcaaa
     1861 aatccaattt gtcgaattgt tatatattca tattgtaaca taaaaaatag ctaaaaaatt
     1921 catgcacttt cattgccaat aaattttcaa ggaaatatga aagtttaaaa ctaagaataa
     1981 aaagaaataa aaaggaagct actaatcaaa atattcaaac acaagtaatc aattatgata
     2041 ttaccagcag ttttaaatac aaatataacc acatatgcct caaaaacact tattatagta
     2101 tcttagacac ttagacaata cacaagattc attaataaaa aagttctcaa tatagatata
     2161 tttaaccatg ttttgaatag cagataatta tatgattaaa aattaaatat gacatagcgt
     2221 ttgtggaata taacacctaa attatgttat aataataaaa caggagtgtg atagtgatgg
     2281 atgaaaaaat attagaatta ttacaggaaa tacaaggtaa tattagtaat atgcaaggta
     2341 gtatcagtgg tatcgaaggt actgttaatg gcatgcaggg taatattaac tatatgcagg
     2401 gtaatattaa ctatatgcaa ggtactatta gcgatatgca aggtactatt agcgatatgc
     2461 aaggcagtat caatggtatc gaaggcacta ttagcgatat gcaaggcagt atcaatggta
     2521 tcgaaggcac tattagcgat atgcaaagca atatcaatga tatcgaaggt actattagcg
     2581 atatgcaagg cagtatcagt agtatggatg gcactattaa tagtatgcaa gacagtatta
     2641 ctgatatgca aggtgaaatt aaagaagtca atagtagatt agacaatatg gaacctgata
     2701 ttaaagactt aaaaactgga caagttgaaa ctagtagtag acttgataca attcagagtg
     2761 atgttacaga aattaagtca gatgtaaaaa ttaacagaaa tacattagat acagtgatta
     2821 aacatacctc ttcattagta gaagatacat cagatattaa aaatgattta aacaatatag
     2881 agatagtcac atctaagaat tggaatgata ttgctaaatt aaaatcagtt aaataatata
     2941 ccatagtaaa aaatacctga aaatttcaca tacttcttta aagttatacc aaattttata
     3001 cagaaatact gattatccga caacacactt tacttataaa taatttatta aaaaatactc
     3061 attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa aaataaggga
     3121 gattgaacac taaaaagttg tgtatctccc ttatattcta taatatagtt atataatttt
     3181 tcagcctaaa ataaaagtcc atctttgatt aaaacatatc cccttatcac ttataaaagg
     3241 ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa taattttttc
     3301 ttttgatact ttaagtgttt ggttaaattt aaccaatcga ctaagttttg ttcaacatct
     3361 atttttcata ttttccaata cttaatctat tttttataaa taattctttt atttctaact
     3421 cacttacatt ttttacataa aacgaagcat ataaatttca accttatctt gtatggttct
     3481 taatacttaa tgagtagctt aagtatcaat cattataaat tttcccctta tatttaacac
     3541 tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg atgaaataca
     3601 atacatgaaa atacaatatc ataactgtta tctaaaaata gaagttcttc aacataacct
     3661 ttcacaaaga acatattatc taagtatttt ttgtctgctc cttgttttcc tgtttgtagc
     3721 atataaatta ctgcatctaa acatataact actcacacga aaaaagaaaa taaacttcca
     3781 aaatacaagc tctaaaacta cctctaaagt tatataatgt ttactaggaa taaggttgta
     3841 taacatatag tttaaagaat agttttttaa aattgcaagt tattgctttc aaaattagat
     3901 gtttatatat caaataaatg attaagaatt tctaaattta atggctttat aatgcactcc
     3961 ctttctttgg gataaaataa tgttgcacag atactttttc taaagataac aattttattt
     4021 ttctatcaat tccaccagca taccctgtca aactcccatt tgttcctaca acacgatgac
     4081 atggaacaat aatagagatt ccattatgcc ctactgcacc accgattgct tgtgctgaca
     4141 ttttagatat tcctttttgt tgagcaactt tatgtgcaat ttctccataa gtgattgttt
     4201 taccatatgg tatttgttgt aaaattttcc acactgtagt ctggaaagga gtacctacca
     4261 tatggagtga tggtgtaaaa ttgggctctt ctcccgaaaa ataaatatct agccatttct
     4321 ttgtctgctc gaaaattggc aattcttttt cttgatgttc ttggtcaaga ttataagcat
     4381 aatacttttg attctcaaac caaagaccag ttaacccaac attatctgat gctaaaagaa
     4441 tatttccaat tggactatgg taatagctaa tatattgcat acaattcctc cttatattct
     4501 gaaaacttca ctcaaatcat tagacacttc ttttctatct gccattatgt aaaaacatac
     4561 taatataatt tccttctgac tacactttcg ttataatatc tatcctcata tcatattagt
     4621 tttatattta cctgaatctg ttcatccttt ttttcctttc cctttgatgg atataaaatt
     4681 catacattta agcttcctac tgtattaact tttatagaag tgtatattat aattttatca
     4741 ctaaatagac aaaaaaatat cgatataaca tcaagaaata ttttactgag tttcgcttat
     4801 taaaagcaac tgaatttcta aagggtacag atgagctaat aagtgatatt gatataaaag
     4861 ttgtttttca ttaagtaatc tattttagta aatgttttaa agaaaaaacg ggatattctc
     4921 caaaagtgta cagaaatata aaaaaagaga tatgattttt tatttctttt gattatccaa
     4981 agagtaaata aaaaatattg taaaaaactt actaacaacg gcaatgcttt gaactatggt
     5041 aatactttga acttttgatt ggactttttt tgctaaaata atatatttaa atagcaaagt
     5101 aatctgttta atcaataaaa aataagagat aaaagaaatc aactaaaacc tttacctctt
     5161 attttgatag tttatcagag ctaatattta cactcttttt atttttgata cttcattaaa
     5221 aatgatgctt attctctttt catttttaat acttcattaa aattgatgct tactctctat
     5281 tcacttttaa tacttaatta gaactgatgc ttacactctg taaactcata aacatccatt
     5341 aatgcttcac caaatctttg atagtgaact acttccctct cacccaaaaa tctcaatata
     5401 tctttcaaat cagggtcatc agttaaattt attaattgat agtacgtagc caatgctttt
     5461 tgttcagcag ccatatcctc atgtaaatca gttaccgaat cgccaaagac attaatataa
     5521 cttgtagtaa agtttactcc attagaatct actggttgaa gaccatgtcc aaaattagca
     5581 taattactac caagtccagc agccttaagc tcttccggag tacaactgcc tattaattga
     5641 tataacactg aagaaattat ctcaacatgg gccatttcct ctgtacctat atcagttaaa
     5701 agtgcgcgag attttccagt tggcatagta tatctttgtt gaagatatct agttgcagct
     5761 gccaactcac cattaggtcc tcccaactga gtcatgatat attttgccat tctagggtct
     5821 gcttgtctta tgttaactgg atgttccagt gttttttgat aaatccacat attatcttac
     5881 tcctccttaa tatatattta ataataattc taattttaaa acttaataat tttatttata
     5941 aaatgatttg tcccatggcc aaggttcaga tatccatgac caaggatatt cacttgattc
     6001 atatccaaaa ttagttaatg ctccatattt agattcataa tcacacacag cttgtgtaaa
     6061 tttatcgcag tatctattgt ataaatcaag agctttctta tcccttggat tgttatctaa
     6121 atataaattt aattctatac aggcaaattg atattctgaa attctctcta aaagttctct
     6181 tctatttaat gacattagta catgttaccc ccttttttag ctctaactct atttttagga
     6241 tttgaatata gccaatcatt atagttattt gactctatta aatctaattc aggaaataaa
     6301 gtacctcttt ttaatgctgt ttttggattg tatgtgtcat cataaatttg acgattgatg
     6361 tatggtctag caaattcaca acaatagtct tgtttatcct cttttaaata tcccattaat
     6421 tatcctcctt aatttttaga attttaaaat agcattattt ttaaaaattg tatacttgtt
     6481 ctatattata gtcagtagtt ttaataggtc tttaataaga ttaaataatt aaataagtat
     6541 ctttttttgt tcttatttta cctataaaaa ttttcctaat taatctttca taaatatcta
     6601 ttttaaataa ataatataga gtttatacaa ttttttataa ct
//
LOCUS       DAMKPG010000147         6547 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMKPG010000147 DAMKPG010000000
VERSION     DAMKPG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.153"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            357..1631
                     /locus_tag="PC621_003433"
     CDS             357..1631
                     /locus_tag="PC621_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF4110545.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1838..2155)
                     /gene="trxA"
                     /locus_tag="PC621_003434"
     CDS             complement(1838..2155)
                     /gene="trxA"
                     /locus_tag="PC621_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF4110546.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2422..3174
                     /locus_tag="PC621_003435"
     CDS             2422..3174
                     /locus_tag="PC621_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF4110547.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKN
                     KSVEYVLNIKGDKNILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3161..4042
                     /locus_tag="PC621_003436"
     CDS             3161..4042
                     /locus_tag="PC621_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF4110548.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4268..5623)
                     /locus_tag="PC621_003437"
     CDS             complement(4268..5623)
                     /locus_tag="PC621_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF4110549.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2519 a          943 c          823 g         2262 t
ORIGIN      
        1 tctaatcaat ttctttacta tttttacaac tttattagtc atattcataa ttatttctaa
       61 ttagtgttta acattttatg taaaatttat aaaaaaatgt atataaatta aaataagtga
      121 ctatactaaa aatatgatac ggataaacaa taagttcaac ctatctcccc caaaatcaag
      181 tgagctacag tttcccccaa ctgtagctca ttttcgtgta atttaataaa catctcaaca
      241 agaattaatt aacaaaaccc cttataaaaa cataacatat attaaacaag ctcttgatta
      301 aaacttgagc ttttttaata aaaaacagaa tagtttatta gataaggggt ttttttatgc
      361 aattcatgga tgtatctaaa tacattgaag gtgcaaacct taaagttaaa actaaaaatg
      421 gtgaaactgt taaaagaata tattttaaca actctgccac tccattagtt ttaaaaaacg
      481 tagtggataa cttaaactgt gagattcctt ggcttactta tattaatgct ccaggaataa
      541 tctcagaaaa aaatacttta aaatatgaaa atgtacgctc tactatcctc aaacttattg
      601 atggagacga agaaaaggat tctgctattt atgtcaaaaa tgctacagaa ggaataaact
      661 tcttagcaaa attttttaaa gatgaaaatc cagataaatt tattattacc acagcaatgg
      721 agcatatggc taattaccta ccttttaaag ttaattttcc tacaaaagtt gttggaatta
      781 cagaaaaagg agaacttgat ttatgtcaat tagagaatat acttaaaaat tatgctggta
      841 atgtatctct cgttacagtt acaggagcta gtaatgtcac aggtataacc actcctatat
      901 atgaaatagc aagaatagct cataagtatg gtgcaaagat attggtggat attgtgcaag
      961 taattcagca taaacctttt tcaatgatgc cacatgaatg tgatgagcat atagattttg
     1021 ttgtttttag tgctcataaa tgttattctc cactaaatgg aggagctctg gtaggaccta
     1081 aaaaattctt tgaaaaattt aatcctgtac tatatggctc aggttctacc aaatttgtaa
     1141 atgataacaa aatactatat gcaaacgtac cccaacgttt tgaagcagga tacccagagt
     1201 attttggaac tcttgcaatg ggaaaagcac tgaacacttt aaataaaatt ggtctaaaca
     1261 gaatttcaaa atacgaaaga gaattatttt tatatgctaa agaagaaatg agtaaaatac
     1321 ctaatataat aatatatgga gataaatcaa ataatgtaat tatcccaaat atatctttta
     1381 atataagtaa tatgtattac aaagatgtag caaaatattt agttgataac tttggaatag
     1441 aaactggagc tggagtagta ggtgcagata tttatgtaca aaaattatta ggtataagtc
     1501 caaaagaagc atacgtcaga tttatgaggg aaaatcctgt aggtcttgtt aggataagct
     1561 taggaatgta caatactttt gatgaggtgg atagctttat acatgctatt aaaactttgg
     1621 cttttaaata agctttttct attctttttt atcaataaat agagagtagt tttgttttca
     1681 caaaaaaaca aattatatca acagtcaatt tcattagcaa ttagacactc atttgtaact
     1741 atacaaacta ttttacacta tacaaataaa aaactcatta caatgagttt ttttgtttat
     1801 atatgatatt tattttaatt acttcaattt actttaatta catatatgat tcaattttgt
     1861 tttttaaatt ctccttaggc ataaaaccta ttaatctatc tacaggtttt ccatccttaa
     1921 atattagcat agttggtact gtagatactt caaatttctg agctaattct atactttggt
     1981 ctatatccac cttcaagaag tttgcttttt caaccatctc atttcctaaa gcttcatata
     2041 ttggtgatag catcttacaa ggtccacacc aagtcgcaaa gaaatctaca actactaaac
     2101 catctttatt ttctacttca tttataaatt cattattatt tattattttt accataatat
     2161 acatctcctt tattatcttc ttgatactaa tatactacgc cctatataac agttcaatta
     2221 ggcacttttt tgtaaccgcc tatttttaat agatttaata ttatgataaa tagatttata
     2281 agattaaata tttataagac taaatatttc tgatatattt tatttttttc ttaatatagt
     2341 tgacattttc tcccttaaat tataaaatca aattaataac tacataggta gttactaatc
     2401 ttttatcaaa ggagtttcta tatggataat ataattaatg aattacaaaa tataggattt
     2461 actaaatacg aatctcaaat atacatatca ttattaaagg aaagcccttt aacaggatat
     2521 gaaataagta aattatcagg tgtaccacaa tcaaaagtct atgaaaatat aacaaagcta
     2581 ttaaacaata atataattat caatgttgga actgacccta ttaagtacat acccataaac
     2641 cctaatgaat tattaaaaaa taagaaaaaa gaatttgata attctatgga taatctaaag
     2701 aatagcttac agatattaaa taaaaataaa agtgtagaat acgttttaaa cataaaagga
     2761 gataaaaata tattaaaaaa agcattagaa atgataaaaa atgcaaaaca agaaattata
     2821 atttcttgcc cttatgaaga aattcttgaa ttaaaaaaag aactcctaat tgcttacaaa
     2881 aaaaatatta atattgaaat actactttta gaaaacaaaa gtctgaaagg tcttgataat
     2941 attcatactc atggaggtaa taaaactaca cttgaaaata aaatgcttta caaaggaatt
     3001 attttgattg tagataatga tgaaatattg acaggaaact tatcagaagg cagtgaggcc
     3061 atttcaattt ggacaaaaaa ctctaacatt ttatatattg gagttcaata cataaaacat
     3121 gaaatttata tttcagaaca aataaaagga ggttataaaa atgaacaaaa ttaagattaa
     3181 acaagttgat gcttttacaa ctataccttt tggtggaaat cctgctggtg tcgttactga
     3241 tgcctctaac atttctgatg aaattaagca gaatgtggct aaagaaatga atttatcaga
     3301 aactgcattt gtttcagagt caaatgttgc agattttaag gtacaatttt tcacaccagg
     3361 tttagaagtt gatttatgtg gtcatgcaac aataggtact ttccatgcac ttttggaaga
     3421 aggaaggctt aatactaata agagtatatt ttatcaagaa acaaaagctg gtgtattatc
     3481 tgtagagctt aaggaaataa ataatgaaaa tatatttatg atggaacaga atacacctga
     3541 atttgaaaat ttagataatt acagaaaaga acttgcacaa atgataggtg tgcatgaaga
     3601 tagacttctt gaatatccta ttataaaggt aaatactgga ctttggtggc ttgtatttgg
     3661 tctaaaatct ttagatgatt taaaaaatat ctctccagat ttaaataaaa tcaaaaaatt
     3721 tagtggtgat aataatataa ttggtataac tccattttgt atagaaacta tagataaaaa
     3781 gtgtgactac catttaagat ctatagcacc atatgttgga gttacagaag accctgtatg
     3841 tggaactggg aacgcttgcg tatcatctta tatagtacat cacaatattt tagataaaac
     3901 tagttttatt ggtgagcaag gtaactttat aaacagacct ggaaaggtat atgtaaatat
     3961 aactagagaa aacaatgaca taacttctgt aaaaattgga ggaagtgctt atactgtttt
     4021 aagtggtgaa attatgtatt aatcccatac ctaataaaaa caaataaaaa tactagctaa
     4081 ctaaagctag tatttttatt tgttattttt atttaataat cacactctaa caaatattat
     4141 aattatatat atcataatat tgtatttaga agtaaaattt tatttttatt attaattagt
     4201 tataaatcaa tctaatacaa cataattaca gtagattaat ctataaaaca taataattac
     4261 ttactgatta tttatcatcc tcaacaagtc tacttaatgt cttatcagat aagaataata
     4321 atatcgctgc aacaaatagt attactggaa ttatagtata tactttaatc atatcaaatt
     4381 taagagtaaa tgcatatata tatccatatc ctttaccagc tataaaagta gcaaatgtcc
     4441 atactcccat aagaacacct agaagcttct taggtgcata cttacttaca aaagagttac
     4501 caagtggtga gaataacatt tcaccaagac ttaaaagaat accaaacatg attatccata
     4561 gtatactagc tttagcagtt tcaggagctc caactcctct tgaaaattct gcaccaacaa
     4621 gcattaagaa tgcaagacct aagaatatta atccaagacc tgtcttttta aataaactca
     4681 tatctccttg tggtctacta gcaagtttaa accataatgc tccaagaact ggtcctaata
     4741 ctatacatac aagtgaattt aatgagtcaa accaagcaag aggaacatca aatcctccaa
     4801 cattcatgtt tacaaatgct gcaccataat cataaactgc aagataagtt agataccaga
     4861 atacccagaa aattacagag aagaatgata taagtataat agcccaaatt ctcttcttat
     4921 ccatgcttgt aagaggagac ttatcttttt cttcaacttt ttcagcaact acaccttctt
     4981 taaaaggtct ctttcctgca tttccaagga atctccatcc atatatgaac catattgcac
     5041 ctattacaca aagtacagct gctataaaga aacattgaga aaatccaagt acaccatttt
     5101 ttgcaaatgt tttaaggtaa agaataccaa ctgctatagt accaataaaa gaacctatat
     5161 taacaaaaga atattgtact gaaaatgctg tatcaagttc ttcttgacta tcaaaaagct
     5221 gtccatttac cgcagatacg ttacctttaa agaatgcagt accaatcgaa actaagatta
     5281 tcattgcatt taccatagaa actgtagttg caatagaacc aagatagtaa cctaccccca
     5341 tgataagcat acctataggt atagtatatc tagctcctat ccatctatct gatatatatc
     5401 ccccaattat aggtgcaagg tatgtgaatg caacaaggtt ggcttgcatt atagcagctt
     5461 gtcctttatc tattccaaga ccaccatgaa ctatagctgt tgtaaggaat atatatatta
     5521 agtactttga agcataatat gctgctcttt caaatgagaa agttgttgag cagatataaa
     5581 atcccatagg aaatttaccc ttctttttta ctgttgagct cataatttta aatcctttct
     5641 taattgtatt tttatttttt acgttttgtc caaattcttt cttttatgaa agatataata
     5701 ttattatttt gtaaaacaat ttgtaattat tattaagata attgcgcatt tatttgtaga
     5761 gtacatattc tcatggaata aaattaatac acttcttaat cataaattta aattatataa
     5821 attcactatt ttcagatata gttataaatt aatatattct catctaatac catgaaaaaa
     5881 ccttcgccat atgcaattct tattttttac gtgatagtat tttacaaact aaaatccccc
     5941 ttttgtagca acttctgtaa ttacatttta gtgctttttt aaaggagtct cgaataaagc
     6001 aacatatatt ttgttcaaat aatgttttta tttacttttt aaatttatat attacttcac
     6061 tcaaccaatt ctcaacttaa ataaataaac tactatctat tcacagatta gacctatttc
     6121 ttacacatta gtttataata aacactgtga atctaaacat aaaaactgtt attttaaagt
     6181 aattacccat atattcgctt tgatactatt tattgaggtt atactcttta tttataatat
     6241 attatttata aaatacctca aaatatatta ttaataaaat acctcaaact gaaaactttt
     6301 tcacgaatat ttatttttca cctccccttt tttattttac catgtaaatc gtaccataaa
     6361 tatgcactta tatgcaagaa aaaaatctat taatcgacag taaataatta gaaaaattta
     6421 aattatattt gttaagtgta ttatataaaa tatttcctat gaaaatataa attaaattaa
     6481 tgttcagaat agtttttaga tatgaaatct tgattttttt attactttac ttatttgctc
     6541 aaatata
//
LOCUS       DAMKPG010000148         6521 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMKPG010000148 DAMKPG010000000
VERSION     DAMKPG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.154"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            9..911
                     /locus_tag="PC621_003438"
     CDS             9..911
                     /locus_tag="PC621_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF4110550.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1520..2533
                     /locus_tag="PC621_003439"
     CDS             1520..2533
                     /locus_tag="PC621_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF4110551.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFEIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2889..3458
                     /locus_tag="PC621_003440"
     CDS             2889..3458
                     /locus_tag="PC621_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF4110552.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(3795..5132)
                     /locus_tag="PC621_003441"
     CDS             complement(3795..5132)
                     /locus_tag="PC621_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF4110553.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(5487..6197)
                     /locus_tag="PC621_003442"
     CDS             complement(5487..6197)
                     /locus_tag="PC621_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110554.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         2404 a          684 c          993 g         2440 t
ORIGIN      
        1 ttggaaatgt gttgccacaa gattattatg gattaatagt gattttaggt gttattcttg
       61 gagttatggg ggcattttac aactggttta ctctatatgt gcagtcttta tacaagaaga
      121 ttcctaaaat tgctaccttt ggaaaactgt tgattccatt tataatggct ggtattcttg
      181 ggattgttat gcctgatgtt ctggggagtg gacacaattt agttgaagaa cttacaagtc
      241 atgaatatat ttttggaatg gttgttttga tttttgttat acgttttata ttttcggctg
      301 ttagttttgg gtcaggtgca ccagggggaa tattttttcc attgcttgtt ttaggagcat
      361 ttataggtgg aatatttggt atggtaggtg ttaaagtttt tggaatgaat cccgattatg
      421 taaataactt tgtgcttttg gctatggcag gatattttac agctattgtg cgtgcccctt
      481 tgactggtat catattaatt tttgaaatga caggttctgt tagtcaaatg ctttcactat
      541 cagtaatttc aattgtggca tatattgtgg ctacacttat gaagtctaaa cctatttatg
      601 aaagtcttct tgagaggtta cttgaaaaaa atggagaacc tgtacataaa gagaggggcg
      661 aaaagatact agaccagttc tctgttatgt ttggctctcc tgttgcgaac aaggcaattc
      721 aggatgtaca gtggcctgaa aattgtcttt tggtagcaat tcaaagaggt ggagaagaaa
      781 ttattcctag aggtaagaca attcttagac caagtgatgt aattgtgact atgacagatg
      841 agagagatag tggtgctgtg tatgataaga tggaaaattt gtgtaaagaa catacgtcac
      901 atatttttta gggataatga tgaatgttta ataatgatag aatatttaat gaagatttga
      961 ttaaaaaatt ggtaatttga aagagagtta aggaattaat agaaaatgaa aatgtgatat
     1021 cttagatgta aaagagtttg aagaaaataa ttgtatatat ttgttaatga gattatatgg
     1081 actaatggta tatttatact gtgctaaaag ttgcactatt gtatgaggaa tgggaataaa
     1141 gggtttgaaa aaataggtag aagatttttc agatattctt attgggatat tttaattaat
     1201 atattttaat tgcgatattt tgggaagaat agaatttatt tataaatgaa aataattaaa
     1261 aggggttaag gttgtattag ccccttttgc ttatagagtt tggaaatggt atcttgatag
     1321 cagttgtttt atgcttatta tattttatta catattttaa aattaacctt atcactccta
     1381 aatcattatt ctatcaactt ttaaaacact ttcaagacaa tcacctattg aaaacaaagt
     1441 taattaaaaa cctacatatc agtttagtat tcgtgtagat gattatataa atataaaagt
     1501 ataagaaagg tgagtatctg tggttagttc atttttaaat gatatcttta gaacaatttt
     1561 agtaagttgt atttttatag ttattttatt agtctttaga ataactttat ttaagacttt
     1621 cagtaaaaaa tttaattact atatatggct tattgtgatt attaagctat cattaccatt
     1681 tatgaattat acttttatat ttaatgagtc aaaatataat ggtaatatta ataagataag
     1741 tttaggcaat tttaatagta tttctattgt atatagtact gttttagtct ctgtttggat
     1801 tgctattacg atattttatt tagtttatac tttattgaaa tatattaagc ttaaaaatat
     1861 gataaatgat ttgtcttatg atgttgagga tgataagata aaaaatcttt atgaaaattt
     1921 attaaaagag cttaagataa ataaaaaaat acggcttaaa tattcatatg aagttgaaag
     1981 tccagtattt tttaattcgt gtgtcatttt accaccatgt gattatacat taaaagagtt
     2041 aaactggata tttaggcatg agttgatgca ttttaagagt aaagaccttt acattaagta
     2101 tttggtgata tttttaaaat gtgtttattg gtttaatcct tttgtgtata taatggagaa
     2161 gtttatagat ttggattgtg aattttattg tgatgaaaga gttttgaaga attgtacgat
     2221 agaagagaag caggattatg cattgacaat aattagtgca atgagaaaag gttcaaatag
     2281 ttcaaataaa tttgtggctg gattacataa ggagtcagat attaaaaaaa gagtgtttag
     2341 tatgtttaat gaaaagtaca gaaatggtat tttgatagca gttattttat gtttattatc
     2401 ttctattaca tattttgaaa ttgatttaat tactactaaa tcaatatttt atccactttc
     2461 aaaacaaaca cctattgaaa atagaactaa ttcaaaacct acatatcaga ttaatgttta
     2521 tatagataat taaaaaatac aaagtttaac aagagattaa tataaataat tttaataagt
     2581 atctttcaca tatgttaaat ttactttaat tatattaaag ccaatgaatt attctttaga
     2641 agctccaatg ccagtaaata caggtaaaga aaaatctttt actacaattg taaattaaaa
     2701 aatattctaa tatttaaaga aatgcaaaag tgtaagaagg gtaaaaatct gttataatac
     2761 attaaaaact aaatttagaa aaatttacat aattagaaaa atgatttgat aaaaaataaa
     2821 gttaaatatt aactaagtaa atttgaaaaa gaaaaaatat aaagtacaaa tttaaaagga
     2881 ggtaactaat gaaaaagaag ttggaagata tgactttaga ggagttatgg aacttgtttc
     2941 ctatattttt gactactcac aaagaatgtt ggaaaaattg gtttaaagaa gaacaatata
     3001 gactaactgg aatattgcca atgcaaaaaa ttgttagaat aagtcatatt ggaagtactt
     3061 ctgtgagtac gatttatgcc aagtccattg ttgacatact gattgagata attcctgatt
     3121 ataatatgaa agatattaaa gatttgattg agtcgaatgg atatatttgt atgtctgaga
     3181 gcagtagtag gctttcactt aataaagggt atacagtaga tgggtttgaa gaacgcgttt
     3241 ttcatttgca tataaggtat gtaggtgata atgatgagtt gttttttaga gattacttaa
     3301 ttgaccaccc agatgttgca aaggagtatg aaaaacttaa gttggacctt tggaagaagt
     3361 atgagcataa tcgtgatgct tatacagata gtaaactaga ttttgttagg gagtttactg
     3421 aaaaggcaaa ggtgttgtat tctaatagat ataattagaa ataggtacaa ttagatattt
     3481 atcttatata tcgtattata ttttttaaga aagtaattaa tagaaaaagg ggttgtttca
     3541 aaatggagag ataaatccct ttttcattgt tgaaatgata aaaatatatt tgatttcatg
     3601 taaaaaagag tgcctcatca actaaaaagt ttattttgag acacacacat ttttattaag
     3661 gtaaataaaa atatcacatt tgattaaatt ttaagttaat gaatatatgt gtaaatattt
     3721 aattataagt tattataatt ttacatagta taaaacaatc taatttgtat tatataagat
     3781 aatctaattt tagactaaaa taatctaata taaagaaata gagaaaattc attctcagta
     3841 taagagatat ccaattttcc attgtactta ttaacaattt ctctaatatt ctcaatacca
     3901 tatccgtgag aattactatc ttttttatct gaaataaata gttcttgttt tctattgata
     3961 ggatttacct tgttgtttgt caataaatag ctaaaatact ccttctcaca acgtgccttt
     4021 aataccactt ttctatttga caaatcagga attttcaaag atgcttctat agcgttatct
     4081 aaagaatttg aaaaaatagt acataaatct ataggttcta tacccaagtg attgtctata
     4141 tctatattaa agtgcacatc aacaccattt tcttccaaac ttgtatgacg aagatttaaa
     4201 attgagttaa gagcttggtt tttgcaaaat tgtttgccac taagagtacc tatacctatt
     4261 tttaactgat taaaatactt tttagctcca gcaatatctt caatttctaa atacgattca
     4321 atcatttgaa agtgattatt catatcatgt cgcattttgc gtatttgaat ttgttgattt
     4381 tctagtaagt tatagtattt ttcttttagg tgatttgctt gtttttctat cttcatcttt
     4441 acattttcaa tcaatggctg taaaagaagt atcacaccga tatttgccag aattgctgac
     4501 aagatgatta gataagtgcc ataaggtaag ttgcctaaga taatattctt attactaccc
     4561 atagggatta aattagtagg tggcagaata atagcaatta atattgataa aaatgaagat
     4621 atacatacca tatcaataaa aatccatatt ttatttgata agtattgttt tatattgacc
     4681 aatctattct ttaggaaaat atgtattaaa aaccaaaata ataatttaat tagagcagaa
     4741 attatctgga ctattgaaag tattaaaata tattcttttg agtgtataag ttcctcatat
     4801 atagggttgt ttataaaaag aaaattaagc ccaattataa taggataaag tatcatgaca
     4861 actgatatta taggaattaa tttgcttttg ctacatatta taagtagtat aaaatatccg
     4921 aatagtgtaa acaaaatatt tacggggtcg gaaagccata ttatcatttg actaataata
     4981 gagtagccca aaatcaatat gattttgaaa atccaatgat tcctaaagtc tataaaatag
     5041 gaagtcaact tataaagaaa aaaggctatg aaaatattga ttaatattag aataaaattt
     5101 atgcttgtag agattaaaga ataagtgtcc atttaatata cctttctatt aattaagatt
     5161 ctggaaacta agttttaaaa aattaaatct taaaaattaa aattctggaa attagatttc
     5221 aaagaattga ttttgaaaaa taaaattcta gaaattaagc ttcaaaaaat tgagttttga
     5281 aagattgaaa ttttaaaaat taagtttcaa aaaattaaat ttgaaacaaa actctaaaaa
     5341 ctaagcttca aaaaattaaa ttttgaaaga ttgaaattct agaaattaag cttcaaaaaa
     5401 ttaaatttga aaaaacaaaa ctctaaaaac tgagctttaa aaaattaagt ttcaaaaata
     5461 aaattctaga gattgaactt caaaaattaa ggttctaaca tataccttgt aaattttcct
     5521 acaacctctt gatatttttt acggcttata ggaatttttt cattctgaac tttggcataa
     5581 gtttgagtta tactatctac atggtttaga ttgacaatat acctttgatg cgtacgtata
     5641 aaattagagg gaagaacagt ttccatatca tttaattttc cgtagtattc taatgtttta
     5701 tcaaaggtaa ctacagttaa ttttcttagc tcgcttttaa agtagaggat atcttttgta
     5761 ttaattttac aaagattagt tccaatatga actgtaataa agttttcttt ccattgttct
     5821 aattttttta gtgcttcttt taatacttta catatttttt cagttttata aggttttaag
     5881 atgtagttca aagctcctac atcataccca tgaaatacat agtcagaaaa acctgtgaca
     5941 aagatgatta tagtatcttt tgaatgtttt cgtatagttt ttgctatgtc aacgccatta
     6001 cttttttcaa gttcaatatc taaaaaaata atatctatgt aataattttc gtgttctaga
     6061 ttgtatacta gactttctcc atttgaatat tcttttattt ggtaggggta gccccacaaa
     6121 tccatttctc ttgtaatgat tttattcagc atgtttcttt gttgtttttc atcatcacaa
     6181 atacaaatcg aagtcaattt tttatctcct ttcaagtttt atatttatat agttaagtat
     6241 ataatatatt ttgctaccta gatatataaa atgaaataac aattatatcc agatagcaaa
     6301 ataaaatttt ttatattagt atacttacac ttaaaactat tctattaaac ttattctgaa
     6361 gtgctaatga ttttcttatt aaaaaatcta ataacctttc ttattataaa ataccaatgc
     6421 ttttattatt ataaaaatgt aatgactttt catattataa aaagcaaatg attatcttac
     6481 tataaaaata caatgacttt taatactata aaaagcaaat a
//
LOCUS       DAMKPG010000149         6513 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMKPG010000149 DAMKPG010000000
VERSION     DAMKPG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.155"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..365
                     /locus_tag="PC621_003443"
     CDS             <1..365
                     /locus_tag="PC621_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110555.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNCKLSRLISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLSIREWICPSCNETHDRDIN
                     ASINILKEGLRLITTQNK"
     gene            484..1002
                     /locus_tag="PC621_003444"
                     /pseudo
     CDS             484..1002
                     /locus_tag="PC621_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1029..2390
                     /gene="rlmD"
                     /locus_tag="PC621_003445"
     CDS             1029..2390
                     /gene="rlmD"
                     /locus_tag="PC621_003445"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF4110556.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2913..3947
                     /locus_tag="PC621_003446"
     CDS             2913..3947
                     /locus_tag="PC621_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF4110557.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            4034..4237
                     /locus_tag="PC621_003447"
     CDS             4034..4237
                     /locus_tag="PC621_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF4110558.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4408..5568
                     /locus_tag="PC621_003448"
     CDS             4408..5568
                     /locus_tag="PC621_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF4110559.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(6099..>6513)
                     /locus_tag="PC621_003449"
     CDS             complement(6099..>6513)
                     /locus_tag="PC621_003449"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF4110560.1"
                     /translation="DLGDRSIVSYVIGKSNNNNLVFETFNKAIEVYPNAKPIFHSDRG
                     YQYTSRVFKSKLLTQGMIQSMSRVGHCIDNAPMEGFWGILKVEMYYLRKFDTYEQLVK
                     AIDDYIYYYNNFRYQKRLNSMSPLEFRKYLSTEIA"
BASE COUNT         2555 a          681 c         1220 g         2057 t
ORIGIN      
        1 tacaaaaact atctactaaa ttaataaaag agaatgatat tatttgcata gaagatttac
       61 aagtaaagaa tatgattagg aattgtaaac tatctcgctt aatttctgat gtgtcatggt
      121 cagagtttat tcgccaatta gaatacaagg ctaattggca tggtagacaa attgtaaagg
      181 taggtaaatt ctttgcaagt tcacaaatat gcaataaatg tgggtataaa aacgaggaag
      241 tgaaagactt aagtataaga gaatggattt gtcctagttg taatgaaact cacgatagag
      301 atataaatgc aagtataaat atattaaaag aaggattaag actaataaca actcaaaata
      361 aataacttat gtaagaaccg taggaactac ggggatagcc tgtggagaat tagtaagaca
      421 tattttagta tgcaaaattc tgtgaagcag gaaccctgtg actttagtca tgggaggttc
      481 aggatgaata aacctgatgg atatatatca gcaactacag ataaatatga cccaacagta
      541 ttagatttaa ttgatttatc atacttagct tttgaaccat ttcctgttgg aaggttagat
      601 aaagacacag agggattatt agttttaact aatgatggaa agctttctca tagagtttta
      661 tctcctaaga agcatgtgcc taagacttat tatgcaaaaa ttgatggagt agttactgaa
      721 gaagatgtag aagctttttt agaaggtgta gtgcttgatg acggatataa aactatgcca
      781 tcacagttaa atatattaaa aagtgatgat gaatcagaaa tagagcttac aatacatgaa
      841 ggtaagtttc accaagtaaa aagaatgttt gaaagcgtag gcaaaaaagt agtatactta
      901 aaaagattgt ctatgggcaa tttaaaattg gatgaaagtt tagagttagg tgagtataga
      961 gagttaacag atgaagaagt aaaattgatt gaagaaagat aataaagata ctatagtaaa
     1021 ttggaggaat gtctgtgaag aaaaaagatg ttatagaatt tgaagtagat aaaatggaat
     1081 ttggtggtac atctttaagt caagttggag atagagttat ccatatgaag ggtggaataa
     1141 gtggtcaaaa ggtgagagct ggagttaaaa aagttagaag taagaaagct gaagttaaga
     1201 tgatagaatt attagagaac tcaccacttg aaactgaaga aacttgcaag cactttagag
     1261 agtgtggagg atgtacacta ttgtctgtgc catatgaaaa acaattagaa ataaaagaaa
     1321 aacaagttat ggatttattt ttaaagcaag atttatttgg atttcagttt ttagggatag
     1381 aaggaagtcc agaaaacaaa tattatagaa ataagatgga atatactttt ggagatgaag
     1441 taaaaaatgg acctttgaca ttaggtcttc ataaaaaagg aaagcatata gatatacaaa
     1501 ctgttgagga gtgtatgttg gttgatgaag acttctcaaa gatattagtt tctagtgtag
     1561 agtttttcaa tgaaaaaaag cttccttatt atagaacaat gaatcacaaa ggatatttga
     1621 gacatcttgt agttagaaaa gggattcata ctaatgagat tatggttaat atagtaactt
     1681 cttctcaaga agattttgat atgaatgagt ttaaagatat gcttttaggg attgatttaa
     1741 aggctgagtt agtaggtgta cttcatacta ttaatgatgg tttagcagat gcagtccaat
     1801 gtgatgagtt aagagtattg tatggaagag attatataca agaagaaatt ttaggtctta
     1861 agtttaagat ttcaccattt tcgtttttcc aaaccaatac taagggagct gaggttcttt
     1921 atagtattgc tagggatttt attggagatt acaatgataa ggttgtattt gacttgtata
     1981 gtggtactgg gtctattgga caggttatgg ctggtgctgc taagaaagtg tatggtattg
     2041 agatagttga ggaagctgtt gttgctgcta atgagaatgc taagttgaat ggacttacta
     2101 attgtgagtt tattgctggg gatgtggcta aggttgttaa ggatttaaag gacaagcctg
     2161 atttgattat tgttgaccct cctagaccag gcattcataa ggatgctatt agggatattt
     2221 gtggatttgg tgctaaggag attgtttata tttcttgtaa tcctaagtct ttggttgttg
     2281 atttggttga ttttaagggg tatggttatg agattaagat ggttaagtgt atggatatgt
     2341 tcccaaatac gccacattgt gagactgtag ttaaacttat tagagaataa cagggtttca
     2401 gacttttata ttgtgtctta aaagtagatt tgccacctat ttgccaccat aattattttt
     2461 gggtggcaaa tttattctac aatgatttca aaaatattta ccatctggtt tctcatgtta
     2521 ttagttacat gtaaatatgt atccatagtt gttgaagttt tagcatgtcc gagtatatgc
     2581 tggatatctt ttatattagt accattttct aataacatag tatcagggtt tttaaaataa
     2641 agtgtgtatt cttctagagt agtgataatt aaaatatcaa atatcactat acaaaggaaa
     2701 gtgcaccctt tttatgttta gcaaaaacga aatattaaaa aaacttattg aagaatatga
     2761 tgtcaaaaca actacagata ttcaagatat gctaaaagat ttatttacaa atacaattca
     2821 tcaaatattt ccattgtcga attttaacgg tttaaacata atatattgac tgatataata
     2881 aaatcataga atattttagg gggaaataga ttatgtttaa gaaaaagtta atagctgttg
     2941 caatgagtat aactatgatt agtgtaggga gcttttcgta tgctcattca ggaagaactg
     3001 attcatcagg aggtcatcgt gataataaaa ataaatctgg attaggaagt tatcactatc
     3061 attgtggtgg ttatccagcc catttacata ataatggtgg ttgtccttat actggaggag
     3121 gaagcagttc tggaacaaca acttcggtta ataatgaaga aaagcaaaag aggtctgttg
     3181 gagaaaaagg atataatcaa gggtatgaag atgggtataa aggaaattat agttcatcaa
     3241 attatagtgg agattatagt gatacatatg aatcaaagta ctcagaagga tatgaaaaag
     3301 gtaaggctaa attagaagag gaagaaaagg tggctaaaga gactggatat aatttagggc
     3361 ttacaggtgc taaatctaat aatacatatg aaaaagaagt tttaaaaaat gcttatgata
     3421 ccggatattc aactggatac aatgaatata agactaaaaa gatagaagaa tataaagcta
     3481 aagggataga ggatagcaat aaagataaag aaaaaatgac atttgaagaa aacatagatt
     3541 ctgaatttat agatgcttat aataatgctt atgacgaaat acaagaacaa ttaaaaaatg
     3601 attacactac tcaaggattt gagagtgcta tcaaaggaga aagatttgac acatcaacta
     3661 taggtaatgt taaatatgct aattggttta aagaaggata tgataatggt aaggttaagt
     3721 tacctaaagc aaaagaaagt gtatataatc aaggttacaa tgaagaagat tttagtatac
     3781 catatgaaat gaagtctatt gaaactaagt taaaaggtgt atatgatgaa ggtttagaaa
     3841 aaagagaaga agagaaaagt agaaatgcta cttatggggt tggagctgga acagcagctg
     3901 tagttgctgg agtagtatat aaaaataaaa aacgtaaaaa gatatagatg ctagatatac
     3961 caatgatgac aagtgtataa caaatataat tttaagtata aatatttaaa atatagaagg
     4021 gaaggttata tctatgggat ttaactttag gaaaagcata aatctaggag gaggactcaa
     4081 attaaattta ggtaagaaga gtgtaggtat aagtgctggt gttaaaggtg ctagagtgtc
     4141 agttaattca aaaggaagaa aatcagctac attgagtata cctggaactg gaatatcata
     4201 tacaaaaact agtacaaaga aacgtaaacc taaataaata tttgatatac taagaaatag
     4261 actgttacaa atagtctatt ttttagtata tagatttaaa aagtgacaaa attttgaaat
     4321 ataaactaat ttcataaagg aaaatattct tttatgtaga attataatat ttaagtaata
     4381 ttataaaatt tataggagga tttcattatg aaattaagca aaacactatt ggcaataggg
     4441 atgtctgttt cattattgat tgctagttgt ccaacatcaa acgctcttag ttctattaat
     4501 tcaataaaag gtacaaataa gtatgaaaca gcaggtttaa tagcagataa acaaaattat
     4561 gatattgcaa tacttattaa ttctgaaaat ggattatctg atggattgtc agcaagtgga
     4621 ttgtcaggag ctaaaaatgc cccaatattg ttaactaaga aagatacaat cccttcagaa
     4681 acattaaaaa gattagaaaa ggtaaaagaa gtatatctaa ttggaggaaa aaattcgata
     4741 ggagcatcag ttcagaaaat attaagtgat aaaaaaataa gcataaatag aataagtgga
     4801 actgatagaa tagatacaag tcataaagtt gcaaaagaaa taggaagagt aagtacggtt
     4861 aaaaaagtaa tattaactaa tgcttttaaa ggcgaaccag atgctatgag tgttgcttct
     4921 atagcagtta gagataaatc tccaatagta ttaactgatg gaaatactac aagttttgct
     4981 acagatggat tagaaactta tgcaattggt ggaaaagtca gtatgagtga taagttaata
     5041 aatgaaacta aagcaacaag aataggtgga gtagatagat acgatacaaa taaacaaata
     5101 ataaataaat tttataatgg ctcaaaagaa ttttacatag caagtgggac tgatttagtt
     5161 tattctttag ttggttctac aattgcacaa aaaacaccta tagtgttagt tggaaatgga
     5221 agtaataaga gtatattaaa aaatgcaagt aaaataactg ctattggaaa tattgatgat
     5281 gttattataa aacaatgttt aaatataact aataatgttg gggattcaaa tacaggtatt
     5341 gtaaaagata ctaattcaaa taatacaaac aaaccaagta atgagcctag ctctaataat
     5401 cctggattaa ctgataaaag tacagtatat gtaactccta atggaaaatc atatcatcta
     5461 acaaaaaatt gttctacact aaaaagaagt aaaacaataa ataaagtaac attaaaacaa
     5521 gctaagtcac aaggtaaaat agacccttgt aatgtttgtg taaaataatg tattagccaa
     5581 atatatatta aaaaagacaa ttaattaaga ttgtcttttt tattgaagaa atataaattt
     5641 atattatgta ttattaaaaa gaaaattatt aattataaaa aataataata aagacgataa
     5701 gtattaaaat tttatgtttc caatatttat gatataatta atatggcaag gaataaatat
     5761 ttgaaaaagc tatgagtggt gtttccatgg aagtttcttc actttcttat gagaggaggt
     5821 gaaaactatg gaaaatctaa tgatgagtat tataactggt gttatagcca gttacattta
     5881 cgataaatta aagagccact ctgaccaatg aactataccc actgttttat acagttaaaa
     5941 catgtaaaag tggttataaa gtactagtgt caatcattag tacttttttt attatattca
     6001 tataattaat agctatattt taaagtttac tatctaaaaa atagcacgtc aaataaaacc
     6061 ctagatattt aaattttttt agagctactt aaaataaatt atgctatctc tgtacttaaa
     6121 tatttcctaa attcaagtgg agacatagaa tttaatctct tttgatatct aaaattatta
     6181 tagtaatata tataatcatc tattgctttg actaattgtt cgtatgtatc aaatttcctt
     6241 aaatagtaca tttccacttt taaaattccc caaaatcctt ccataggagc attgtcaata
     6301 caatgaccaa ctctcgacat actttgtatc atgccttgtg ttaaaagttt tgatttaaac
     6361 accctgctag tatactgata gcctctatca ctatgaaaga taggttttgc atttggatat
     6421 acttctattg ctttattaaa agtttcgaat actaagttat tgttatttga ttttccaata
     6481 acatatgaaa ctatgcttct atcacctaaa tcc
//
LOCUS       DAMKPG010000150         6493 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMKPG010000150 DAMKPG010000000
VERSION     DAMKPG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.156"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(206..1651)
                     /locus_tag="PC621_003450"
     CDS             complement(206..1651)
                     /locus_tag="PC621_003450"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF4110561.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(1795..2703)
                     /locus_tag="PC621_003451"
     CDS             complement(1795..2703)
                     /locus_tag="PC621_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF4110562.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            2960..3784
                     /locus_tag="PC621_003452"
     CDS             2960..3784
                     /locus_tag="PC621_003452"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF4110563.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3824..4354
                     /gene="lepB"
                     /locus_tag="PC621_003453"
     CDS             3824..4354
                     /gene="lepB"
                     /locus_tag="PC621_003453"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF4110564.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(4436..6217)
                     /locus_tag="PC621_003454"
     CDS             complement(4436..6217)
                     /locus_tag="PC621_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF4110565.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         2100 a          964 c          787 g         2642 t
ORIGIN      
        1 tttaattagt aatcttatca tttttttatt tttatttata ttatttctat taaatattat
       61 ttatatagct attttattaa attcatggta tagtaagtaa agaagaactc ttcacagcgc
      121 tgaagagaag acgctagccc attaaaatgg ctatcttatt tataaataag atagccgttt
      181 ttaattatat tttatctgta caattctata ctcttaggtc cacatctact tttcctaagt
      241 cttccttata atcttttata gcaggttcta caacttcatt tacaaattct acaacttgtt
      301 ctggagctct acctataaag ttttttgggt ccattataga tagtatttct tccttgctca
      361 ttttaaatga atcatcattc attataagtt caattaagtt attatcctta ccttcgtgct
      421 ttactctata ggcagctttc atagaatatt ctcttattat ttcatgtaat tcctgtctat
      481 ctccacctct ttttacagct tccataagta ttgtttctgt agccataaaa ggaagttctt
      541 catttacacg tttatttatc atattttcat atactactaa accatcagtt acatttatcc
      601 ctatttcaag tatagcatct gctgccataa atgcttgagg tattgccaat ctcttattgg
      661 ctgagtcatc taatgttctc tctaaccatt gagtggcttg taccattgca gggcttatag
      721 attcagagat tatatactta gataaagatg ctattctctc acttctcatt gggtttctct
      781 tataagccat tgctgatgaa cctatttgat ttttttcaaa tggctcttct aattctttta
      841 aactttgtaa aagtcttata tcgttagtca tcttatgcat actttgagct atccctgata
      901 gtatgcttat tacttgataa tctaactttc tagtatatgt ctgtccactt actgcatagg
      961 aagaatggta tcccatcttt ttacaaattt tcttatctaa ctcttttact ttttcgtggt
     1021 ctccttcaaa taagcttaaa aaacttgctt gagtaccagt agtaccttta actcctctta
     1081 acttcatgtt atctattcta tagtttaaat cttccaaatc aagaattaaa tcttgagccc
     1141 ataaacaagc tctttttcct actgttgtaa gttgagcagc ttggaagtgt gtaaatccaa
     1201 gagctggcat atctttgtac ttaagtgcaa attcttttaa attgtttatt aaatttacaa
     1261 gctttactct tagaagtttt aaagcttcat tcatttgtat aacatctgta ttatctccaa
     1321 catatgcaga tgttgctcct aaatgtatta ttcctttggc tttttcacat tgtaaaccat
     1381 aagcttttac gtgagacatt acatcatgtc ttacttcttt ttctattttt ctagcttcat
     1441 caaaatttat attatctata ttagctctta gctcatctaa ttgttcttca gttatattga
     1501 ttcctaactc atgttcacct tctgcaagtg caacccataa ccttctccat gtagagaatt
     1561 taaattgtgg agaaaatata taactcatgt ccttgctaca atatctgtct gtaagtgggt
     1621 ttgagtaagt agtatattta ttatctatca ttatattagc accttcctta aatcttttgt
     1681 cgtgtgatta ctgtgtacac attagtacat aaataattat atataatttt acgaactata
     1741 acaagacttt tttatttttt gttcacatct atatatagta ttttttattt attactattt
     1801 tttatttata tccaaattat ctttttcaat taagtaatcc actatttcac tatcaacaaa
     1861 atttaataca tacttatatc taattgcata gtatttatca ctccatccaa gctgattttt
     1921 tacatcactt tcatctgccc caagccttaa acttattgct gcaaatgaat gtctaaaatc
     1981 tcttgctgaa tattgagaaa gtccagctaa atctaatgct tttttgacta taagtctgac
     2041 atttctatct gttatagaat tactcttttg agttgtaaaa ataaaatcat cactaggtat
     2101 tattacctct ggaagcccag aataattcct gtaatcttct aaaagtttaa aaaagtatgg
     2161 atgtaattta actactcttt cttttcttcc cttacctaac cttacatatg agttatcgtt
     2221 ttcatctacc ataaggtctt tccactttaa gctaactaat tcatttaata aacacccagt
     2281 agtaactaga cagactatta ttactctatc tcttatattt aattttggta ggatacctat
     2341 aagtttattt atttcttgta tacttagaac atcatctttt gtttttattc ttgttactgt
     2401 gggtttttct acatatctaa atggatttat atctatgtat ccttcttttt ttaagaagtt
     2461 ataaaaacta tgtaaataac tatatatctt ttcacaagtt gatttcgcat atttagtctg
     2521 tatatagtct acaaaatttt tacaatcttc ttttgtagca taaatcaatt ctttcccttt
     2581 taaaaattct ttaaacagta taacctttga taaataatcg tgtttcgtgt ttggatttaa
     2641 ttttgtagaa aaaacattaa aagtttcata ctcataatca tctagtattt ttgtactata
     2701 cataataact cctttccttt tcataaaata aaatagctat acttttacta taccatatat
     2761 ttccccctca aatatacccc ttattcaata gataactttt aagtttattt tgttatatta
     2821 tgaaggtttt atgaaaatat caatcttcca ttgtgcacta cacagtacta tggtattata
     2881 agttagtaaa aggtggcatt tttttattaa tctatgaata aaatatgcta ctataaattt
     2941 gtattggagg tattttgatt tggaaacatt tagtttgctt gaaattttta aggcagtcat
     3001 tttgggcata gttcagggaa taacagaatg gctaccagta agtagtacag ggcatatgat
     3061 attaattaat gagtttataa aattgaattt ttcaaataca tttataaata catttttagt
     3121 tgtaattcaa tttggttcaa tactagctgt acttgttata ttttttagaa agttaaatcc
     3181 ttttgatagt gcaaagaata ttaaacagaa aaaagaaaca gttcgtttat ggcttaaggt
     3241 tataattgca gttataccat ctggagttat tggaatactg tttgaagatg acatagacag
     3301 agtattcttt aattcaacag tagttgctat tgctcttatt gtctatggta taataatgat
     3361 agggcttgaa aagagaaata aaagacctaa gtataaagat ttttctcaag ttacatataa
     3421 attagctctt tgtataggat tattccagtg tctagctctc ataccaggta catctaggtc
     3481 tggttctact ataataggtg cagtattact tggtacttct agatatgtag cagcagagtt
     3541 ttcattcttc ttagccattc ctacaatgct tggagctagt gcattaaaac ttctaaaagc
     3601 tggatttgga tttactggat ttgaatggct tattttaggt gttggttctg tagtagcatt
     3661 tgttgtttca attgtagtta ttaaattttt tatggactat atcaaaaaac atgacttcaa
     3721 agtatttggt tattatagaa tagtcttggg tatagtggtt cttgcttatt tctttctatt
     3781 ataatgtatt ctaatttaat aacaaatgat aaggagaaaa gttatgagtg ttaaaaaaga
     3841 aatatttgat tggattaagt caatagctat ggctattgta cttgcatttg taattctaca
     3901 atttataaga ccttctgttg taagtggaga atcaatgtat cctactttag atgataaaga
     3961 ttatctgatt ttaaatagga tatcatacaa ggttggtaaa cctgaaaaag gcgatattgt
     4021 agtttttaaa accaatttag ttgatggaga aacaggaaag aaaaaagact taataaaaag
     4081 agttatagct actgaaggtg acagaataaa aatatcaaat tctaaagtgt atgtaaatgg
     4141 aaaattatta aatgaaccat atatacacaa taactatact tctggagata tagatactgt
     4201 tgttccaaaa ggtaaactat ttgcaatggg agataataga gaaaatagta atgatagtag
     4261 attccctgat gtaggtatgg ttgatgaaga tgaaattctt ggtaaggtta tggtgagact
     4321 attacctctt gataatattg ggaaagtgga ctaaaaaata agagactgaa ttggtaaagt
     4381 ttattttaca aatctcagtc tctttttatt ttcttaaatt acaaatattt ttttactaaa
     4441 tatcttttga ttctgtaata agtcttatta tatcacttgc ttcttcatta aaagttgtta
     4501 ctatacttag gtagtctgtt gctctactca tagctaaata aaccttattt aaatctccta
     4561 caaagtcatt tacttgataa tcttgttcaa tatcatttat agtttgattg tataacatct
     4621 ctaattcaca tactattacc gctttatact ctaaattctt tattgcatat atatttgcta
     4681 ttgtagttcc taccttctta cttatatttg tcaaattatc ttctgcacat atataaggaa
     4741 tttttgcctc ttccaatgct ttttttaaca tatattgaaa gtaaatggtt tttccacttt
     4801 ttaatttctt cttattataa ggataaacga tagcaacttc agaataatca agaccttttt
     4861 tacttattaa atactcaatt tcccagatta cagaacttat ttggtcatca aggtcactta
     4921 cttttattat atcaacagac ttatttccac ctctaagtgc tttagtataa aagaaagtac
     4981 taaattctgt atttggtctt aattcgttga tatatgagtt agaattattt gaaaatttat
     5041 ttgtaaaatc tacaatctct ttagcttgtc tataattttt acttaagata atcttatcat
     5101 caaattctat accattatat aattttttaa atatattcaa tctattagag atattcaagg
     5161 agttacaaga acacacatta aatatgaatt ttgttttata taaaaactcc cttataaaat
     5221 ctatttcata ctctaaaaag ctttctgctt catcaataaa aatagccttg aacatatttt
     5281 tattttttat aatgttctgt gcttgtttta caagattatt aaaggtcttt tcgtaatcat
     5341 tttttaacat gttataatct acaattaaat tgaatttttt agctagttta aatataaaag
     5401 aactaaatgt gtgaacttct aaattgttat tatctttgta taataactct atcctctctc
     5461 gaagctcatt gcacaattgt tttgtatgtg taaatatgac taatttatga tgtggatata
     5521 cttttgctaa ttttatagcc ctagaaagca taattgctgt tttgcctata ccagaaccac
     5581 cttcaataag tgtattccca tatttaatag aaacaacatc tttaatttga acgtcttcca
     5641 tcatagttgc tgtatactta tagtcatcat tgtagaatga aattttttta aatttttcat
     5701 ttagatgaag tttatcattt attatatagt attctgaaca tatgtctagt aaaaataaat
     5761 ttaagtttat ttcatcattc tcatcattaa gatactcctc cagcgaaccc ttattcatta
     5821 tatcttgaag cttattttta tctattatat tattattaac aaattcttca aattcatatg
     5881 tttcctcaac ttcaacgtat ggcataatgt aaacgtagtt ataacttata tttctattct
     5941 tttgtcccat ttttagtttc aaaagttcat attcctcatt cataacttca agtaactctt
     6001 cttctaaaat aaaaaatata tcttctgttg tatccataaa ttttataaat aaaattcttc
     6061 catctttaac atacaacaaa tcggtattta ttcctttaaa aggagtaact tttggcacta
     6121 ctttaacgtc ctcattgata ttttcgtaaa atattttctc gaactccttc aattttaggg
     6181 cgtctaactg agaatttata tttatgtatt ggctcactta cattacctcc ttctgttata
     6241 cttaattata ccattttgaa ctatattttt atatatactt tttataggca aatttagtaa
     6301 ataaattcaa aataaataca aataaataca aaactgctaa aataattttg caataaatat
     6361 agatatttat tgttatagaa tactttctgt aaataataaa atgataaatt tcataattaa
     6421 tgtttaaaat agtaagtaaa gttttaaaaa aaataaatct tgttgactaa aaataagatt
     6481 tattataaaa aat
//
LOCUS       DAMKPG010000151         6473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMKPG010000151 DAMKPG010000000
VERSION     DAMKPG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.157"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            265..1461
                     /locus_tag="PC621_003455"
     CDS             265..1461
                     /locus_tag="PC621_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4110566.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(1573..2919)
                     /locus_tag="PC621_003456"
     CDS             complement(1573..2919)
                     /locus_tag="PC621_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF4110567.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(3052..3804)
                     /locus_tag="PC621_003457"
     CDS             complement(3052..3804)
                     /locus_tag="PC621_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF4110568.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(3963..5540)
                     /locus_tag="PC621_003458"
     CDS             complement(3963..5540)
                     /locus_tag="PC621_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4110569.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(5953..6405)
                     /locus_tag="PC621_003459"
     CDS             complement(5953..6405)
                     /locus_tag="PC621_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4110570.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         2275 a         1008 c          798 g         2392 t
ORIGIN      
        1 gctgaaagat tataactaaa aaatcactat cagtgtatca gaatattatc ttactataaa
       61 ttaatttatg aatacataat ttagtggcat aaacatgggt attttactat aatttgttaa
      121 aagtttattt taaagctata ttaatttttt ttgatgattt ttacccataa gtatgttatt
      181 attatcttgt aaagatgtct tgtattcaaa catacaattt acataataca atatacgtat
      241 ttaaattaaa tagggggtat taaaatgaat tattcaaaca gagttagtgc aatgcaagcc
      301 tctccaatca gaaaacttgt tccatttgca caagcagcaa aagataaagg aatcaaagta
      361 tatcacttaa acataggaca accagacata aaaactccta aaggattttt tgatgctgtt
      421 aaaaactttg atagcgaagt cttagaatat gctacatcag aaggtattcc agaattatta
      481 gaagcacttc aaaattacta taaaacttac gatatgaatt ttgaaaaaga tgaattatta
      541 gtaactaatg gtggtagtga agctttactt ttcactatga tggcagtttg tgacccagga
      601 gataatctat tagttccaga accattctat acaaactaca atggatttgg tcaatctgta
      661 aatgtagaag ttaatgctgt tacaacaaag gcagaaaacg gtttccacct tccatctaaa
      721 gaagaaatat tgtctaaagt agatgataaa actaaggcta taattctttc taacccagga
      781 aatccaactg gtgcaattta tacaaaagaa gaacttaata tcctagctga aatagctaaa
      841 gaaaaagatt tatggataat agcagacgaa gtttatagag aatttgttta tgatgggctt
      901 gagtatacaa gctgtggtaa cttagagggt gtacaggata gagttataat aatagacagt
      961 gtttctaaga gatacagtgc ttgtggtgct agaattggtt ctatcgcttg caaaaataaa
     1021 ggtcttatcg ctcaaatact taaattatgt caaggaagac tttgtgttcc tacattagag
     1081 caaataggag ctgttgagtt atacaaaact cctgtttctt acttcaaaga agttaatgaa
     1141 gagtacaaaa aaagaagaga tgtattatac aatgaattaa tgaaggttga aggtgttata
     1201 tgtaaaaaac caactggtgc tttctatata gttgctaaat taccagttga aaatgcagaa
     1261 gatttcacaa tttggatgtt aaaagaattt aacaaagata atgaaacagt tatggtttgc
     1321 cctgctgaag gattctatgc tactccaggt ttaggacgtg atgaaataag attagcttac
     1381 atcttaaatg aaaaagacct tcatagagct gctacattat taaaagaagg tttagagcaa
     1441 tatgtagctt tacaaaaata attttttaaa cttaatttat ataaatttaa tgatataaaa
     1501 tgtccccttt tcagatatta tttgaaaagg ggacatttct taattaatat attaattaag
     1561 acttagacct gactattttt cattttcttt atatagtgtt ggccagtaat ctttattcac
     1621 tttaattaaa gcatctaaaa cttgtcttgc cttagttgga gagattatag ttctatttaa
     1681 tgttaaagat aaaatagctt tttcataaga ttgttcaaag tatgcatcaa ctgctaactt
     1741 ttcataagct agttgacctt ctaacagtcc cttataaaat gttggtatat tacctacatg
     1801 taaaggtctc ggtccattct ttgtaagaat cgcagctact tctaccattg catcatcagg
     1861 taagttgtta atagccccat tattttctgt tataactatg gctatttggt gtttgttatg
     1921 agctattgct tctgcaactt caactataaa ttccccatga gcatcattat gcactaaatt
     1981 agaacctata gatgttcctt tttctataac agatttacat tcttcaaata cacgcttttc
     2041 tcttccattc ataacttctc ttgctctagt acaatttata tttgatttag ctactttttt
     2101 atcaggatat agataatact gtacatatgt attaggtaag taatctggta catcttccaa
     2161 catatcttta accattccat aagtgtctaa ccaagattta tctctttgtt ctgcatcaac
     2221 aggttggaat ccagagtttg ctactaaatc ttttatctta ggtaccaaat cttcaccatt
     2281 tttatcatac aaatgtgtaa accatccaaa gtgatttaat ccaaagtata caggctcaaa
     2341 tgttcttgaa tccatgtcta acaatcttcc ataagacctt aacaagttaa ctggttggtc
     2401 acacatatta agtaatcttt tatcatctgg aaatactctt tttaatgctt ctgcaacaat
     2461 agcagcagga tttgtataat ttaatatcca tgctttagga gagtattttc ttacagtctt
     2521 aaccatttct atcatatcac ctatagacct taacccataa gcaaatccac cagctccaca
     2581 agtttcttga ccaaccagtc caaattgtag aggaatcttt tcatctaatt ctcgcatctt
     2641 aagaccacca gttctcattt gaacaaatac aaaatctaca tctataaatg cttcatctgg
     2701 gtttgtagta tattcaagtg ttgctttagg atattcttct ttaaataata tttttccaaa
     2761 ttcccctatt aatctttgac gttcttctac tacatctaac atagttactt tatttagagg
     2821 aaatctatca ctcattttac ataaagactt taaaagtcct ggagtccaag tactgcctcc
     2881 tccaactata gctatattgt atcttttttt cattgtcata ttaatcaccc ctgtttttta
     2941 ttaactaact tgtaagatga ttataatata taaagttttc tacatgaata cttttaggta
     3001 atcattaaaa atagttcaat tttagtattt ttttcaaaat tgtaattttt ttcatctatt
     3061 gtatttttca atgtatttat gaaacatata ttccataaga gaaagtgctg gcactctaga
     3121 cgtatagtta acactattaa aattatattg tggagaagat acaaataatt ttatatcagc
     3181 tatatttgat aatgaattga cacttatatt tgtcagagaa attactttaa cttgatttgt
     3241 ttttgctata ttacatgcct taataacttg agcagtctct ccagacatag atacagcaat
     3301 tacaaggtca tctttagtaa tatttgatgc agccaaatac atgagagttg aatcatcata
     3361 gtattttaca tttacaccta tagctgaaaa acgttgagac atttcagtac atactaatct
     3421 agaagaccca acaccaaaaa aatatatatg cttagaatta tgtatttcat ttaaacacat
     3481 atctatattc tctacattta ttaaattttg tgtatccata acatcagtaa aaatatcact
     3541 ttgactttta gtaaactcct tatttctttc atttctattt gaatatgaaa aaactaaaaa
     3601 gtttttaaat tcactatatc cagaaaagcc gagcttctga caaaacctta ctatgtatgc
     3661 agtcgaatta aaagtttcat ttgctatatc tttaatcttt ttttgattta ttttttcaga
     3721 attttttaga aaatagttta aaagagatat ttcatttgta gataattttt tttgatttat
     3781 atctaatttg tctattaggt tcatttatac ctccataata atgatttgta gttaaacaac
     3841 tttctcatat ttattatata aatatacact atacaacatt tatcttatgt ttattatact
     3901 acaaaaaaga caatacaaat tataaattag tattgccttt taatatttac attagataga
     3961 cattaaatag acattgcaaa agaaacttcg tctcttactt gagatacaga tggcccataa
     4021 attatttgga tatctttatc agatactttt ataacgccca ttgcacctgt atatttttta
     4081 aaagcatttt catctacttt actcatatct ttaactacta ctcttaagcg agttatacag
     4141 tttgttatat cttctatatt ctccttacca cctaaatatt taattatgga taatgctctc
     4201 ttagtagttt ctccactttg agctatttct actttatttg ataaaggttc taaatcaaaa
     4261 tttatatctg ttatttcttc tcgtcctgga gtttgtaagt taaatctaag tatcaaaacc
     4321 ttaaatacat tataataaac cagtgcatac actaaaccaa gtattataga ataaatccac
     4381 tttgtatttg gttgcataac tccaaatact actaaatcta ttatgccacc ttgagtattt
     4441 cctattgcta ctccaaatat atgcatcatc ataaatgata tacctgataa aaatgcatgt
     4501 ataacaaata atattggtga tacaaataag aatgaaaatt ccaatggttc cgtaattcct
     4561 gttacaaatg ttgctaaagc accagctagt aatatacctt taaccttcga cctattttca
     4621 ggctttgcag ttttatacat ggcatatgct gcagcaggaa gaccaaatac cattataggt
     4681 attttccctt gagctaaaaa tctagttgct tctgttgaaa aactgattgt ttcagggtca
     4741 cttagttgag ctaaaaatat gttcaatgct ccaaatactt cttgtcctcc tacaactaaa
     4801 gaacctccta cttcagtaaa tctaaacata gcatttaata tatgatgtaa tccaaatggt
     4861 attaaaagcc tttcaagaaa tccaaataca aaggttccta aatatcctaa actagctata
     4921 ccaataccta cactctgaat aattgtattt acatatggcc atatatatgg tattattatc
     4981 cccaaaatag agaatactac tgttgttatt attggaacaa acctagttcc accaaagaac
     5041 cctaaataat ctggcagttt tatattatag tatttattat gtaatgatgc tgttattata
     5101 ccaactacaa ttccaccaaa tactcctaac tctaatgttt gtatgcctaa agttacagct
     5161 tgacccattt ctctcatcat gtcaggttca gcaagaatac ctctacaccc taacatataa
     5221 tttacactag tgtgtaatac taaataacca acaaaaccag aaaatgcaga aactgctttt
     5281 tcttctttag caagaccaac agatattgat atagcaaaga atattgctaa gtttccaaaa
     5341 agtactcctg ttatagcttt tgtaaaaccc aatattcctt gaataaaatt attttctaaa
     5401 aaaggtatat cactaactat attaggattt gaaaatgctg aacttattcc taaaaatata
     5461 cctgcagcag atattaatgc tattggcaat aaaaatgatt ttcctaactg taaaaaaaac
     5521 tctttcattt tttgtttcat ttctatccct ccatttttaa atttttaaaa cgttttctat
     5581 gtcataatta tatattttta ttttgttttt gaaactttat aatttgtttt aaaatatttt
     5641 tacataaata gtaatttttt caaaactgta aattttttca aacaaagaca tagtaacatt
     5701 gtgatatata gaaatttaaa aagagattag aaaataaaat tattgaatat aaaatggaaa
     5761 aatataaatt acacaaagag gtatgagtag gtactaacaa ataagaaaat gtataagaaa
     5821 ttataaaaaa gtacatcaag aaaatttaag atgtactttt tagattgtat ttagacttat
     5881 aattatatag ttaaatgaat aacaaagaat taatcttagt actattaaat ataaaaattt
     5941 tttatatttt ttctatctaa attcttcttt gtttaatata tattgaagag tatttattgc
     6001 ttctaaatat ttttttctgt cttcttcagt taatttagaa agtttttctc ctaaaccatc
     6061 acttaattta tccataaaat cagttgctat cttatctccc tcttcagtaa tgtcaattag
     6121 atattttctt ctatcaacta aatctctttt ccttgttaca tacttcttat cagttaaatc
     6181 atcaaccata ctagttaaac tgcctttttc tatatttaac ttagagcata aatcagtcat
     6241 acttattact ccataatttt ttatgaaaac taatgctctt aattgtgttt tatttacatt
     6301 ataatctggt gcaaattttt ttaggtcttc taagtacaga ctagaataaa tcttaggaaa
     6361 tgtttttact aaaaaactta tggtatcttt tactatatag ttcatataat cacctcggtt
     6421 gttttaaaat ttattaaatt atctaaataa ctttatttca gtttacatta aaa
//
LOCUS       DAMKPG010000152         6457 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMKPG010000152 DAMKPG010000000
VERSION     DAMKPG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.158"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     misc_binding    109..320
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            483..1772
                     /locus_tag="PC621_003460"
     CDS             483..1772
                     /locus_tag="PC621_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF4110571.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1826..2905
                     /locus_tag="PC621_003461"
     CDS             1826..2905
                     /locus_tag="PC621_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF4110572.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2926..4470
                     /locus_tag="PC621_003462"
     CDS             2926..4470
                     /locus_tag="PC621_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF4110573.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4633..5175)
                     /locus_tag="PC621_003463"
     CDS             complement(4633..5175)
                     /locus_tag="PC621_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF4110574.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5323..5607)
                     /locus_tag="PC621_003464"
     CDS             complement(5323..5607)
                     /locus_tag="PC621_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF4110575.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5670..6434)
                     /locus_tag="PC621_003465"
     CDS             complement(5670..6434)
                     /locus_tag="PC621_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF4110576.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2243 a          802 c         1128 g         2284 t
ORIGIN      
        1 gaaaatttac atataaaact aaaaaatgta agtaattgac tttttaagtt gacaaaatat
       61 tgcatatata ttatgatata aatcataaat taactacaaa tcaatattat atattcgtag
      121 aagaagaaag tagcataaag gaattttaca gagagttaga tttggctgag aactaatatt
      181 ggaatttatg tgaaaaggac tttggagaat actattgtaa ttatgttaga atagtggttt
      241 aattcgcctt aagaaaatga gtgagtaaaa ttaattttta ctaataagag tggtaacacg
      301 gatttatatt cgtctcttgg atttttccaa gaggcgtttt ttattataaa cttatttcag
      361 aaaatttaaa actatatatt aaatgaaaat tataaaaata tgatagttaa taaatttata
      421 aattagaaaa ttgtaaaata tgctaaggtc agtagtttta cgatttaaat ggagggatga
      481 aaatggaaga agaaaaaatt aactatgctc tggaacagat tccagagaat caaaaacgtg
      541 gttgggtagc tatgttttca gttttagtag caataggtgt ggatttatca tctgttattt
      601 taggcgctga gttggcacag agtatgccaa tgaaacaagc aatattgtca gtaattgttg
      661 gttcattttt ttctgcaatt ttgtatacta catgctcttt ggttggttca tctacaagtt
      721 tatctacatc tatgataaca aagtatgtat ttggagaagc tggagcaaaa atattttctc
      781 tagttatagg ggtttctcta ttaggttggt ttggagttca agtaggtttc tttgcacaaa
      841 atgcacagat aataataaaa gatatattta atttagatgt cagtatgcag attttaagcc
      901 taataggtgg tctcttgatg atgagtactg caatttatgg atataaggcc atggagaagt
      961 tgagtgtgta ttcagtacca tttttattag ttcttatgat gctaactata ttcttagctt
     1021 ttagagcaaa tggaatttca gttgatgata atatgaaatc tactatgaca tttgcaggtg
     1081 gagtttctct gtcaatgagt ataataatag ttggtgcaat tgtatctcct gatataagtc
     1141 gttgggctaa aagtagaaga gactgtgctc tttcaagctt tttgggaata caatttggaa
     1201 atgcttttat gataatagtt tcgatagtat tagtaaaatg tatgggaact tcagatatta
     1261 tgagaatttt tattacatta ggaattgcta taccaggtat aatagtactt acattagcac
     1321 aatggactac aaatacaagt aatgtatatt cagcatcatt aagtatagct cttgtactta
     1381 agaaggctcc tgagaaagta ttaactatag tccttggtat tatagcaaca ttattggctg
     1441 tatttggaat atatgaaggt tttataggat ttttaaattt acttggaatt gtaattgcac
     1501 ctgttggtgg tgtatataca gcagaatatt acatagtaaa gcaagaactt aaaggctttg
     1561 ataaaggagt attatataaa ccaatagtta aacgttcaat agtgtcttgg ataattggta
     1621 tattaataac ttacctttca acatatggat ttataactct tactactata gcacctttag
     1681 atggttttat agcagggttt gtagtacaat ctattattgg aaaagtgctt tgttcaacaa
     1741 aaaataagca agaaatagac aaagcagtat aaagaaattt gtttataaag tataaaataa
     1801 attagaaata tgaggagaaa gtaacatgag atttttaaca gaagaaagtg tagataaaat
     1861 agcagttggt gcagcagtgc ttggaactgg aggtggaggt gacccatatg taggtaaatt
     1921 ggttgcaaaa caagccatta aaaagtatgg tcctgtaaaa gtaataagtt tagatgaact
     1981 tgatgatgat gctttggtag ttccagtatc tggtatgggt tctcctgtaa ttacaataga
     2041 aaaattactt tcagaagtag agttaactac ccctttagaa ataatggaga aattattaaa
     2101 tagaaaagtg gatgttataa ttcctataga aataggaggg ataaactcac ttatgcctat
     2161 agcagttgct gctaaaaaag gacttcctat cttagatgca gattctatgg gtagagcttt
     2221 ccctgaagct caaatggtaa ctttttatct tgaaggatat gaggcatctc ctgccgtaat
     2281 gtctgatgaa aaaggtaatt ctgccatcct ttatcctgtg gatggaattt ggtcagaaag
     2341 attagctaga actttaactg tagaaatggg aggaagttct tctattagtg actataattt
     2401 aagtggagct caagttaaaa aagctgctat aggtaatact cttacaatag ctgaaactat
     2461 aggagggttt ttacttgaaa ataaatctga ttcaaaaggt gctgttgaga atatactaaa
     2521 agaattgtgt ggatacaagt tatttgaagg aaaagtaatt gatattaaga gagagttgaa
     2581 aggtggattt actagaggac atgccttttt tgctgggatt aatgagtatg atggagagta
     2641 ttctatatta ttccaaaatg agaatttaat tgccaaaaaa ggtgatacac cactttgtat
     2701 tacaccagat ttaatagcag tattagattt agaaactgga tttccaataa ctacagagag
     2761 aataaaatat ggctctcgtg ttatggtagt agcttttcct tgtaatgaaa agtggagaac
     2821 agaaaaagga attgagacag ttggacctgg atactttgga tatgatgtgg aatataaaac
     2881 agtagaagaa ttacaaggaa aataaaagtt taggaggaaa ttttgatgta tcgtgtagga
     2941 attgacgttg gaggaacaaa tacagatgct tgtatactgg atggggaatt aaaagtaatt
     3001 cattcagtta aggtggcaac tacaaaagat gttgaaactg gtgtttacaa tgccttaaaa
     3061 aaagttatag atgaaagtaa gattgaccat tcattaataa aatatgctat gcttggtaca
     3121 actcattgta caaatgctat tgtagaaaga aaaagactta ataatgtagc tatgataaga
     3181 ataggaaagc cggccactac tgccattctt ccatttatag attggccaga agacttaaga
     3241 gaaaaaatag aattagattc aatattggta tctggtggat atgagtttga tggtagaaag
     3301 ataaatgaac tttgtaaaga tgaagttgta gagttttgca atagaataaa aggcaaagtg
     3361 gaaagtgttg caataagtgg agtatttgct ccagtaaata aacaacaaga agaagaagtt
     3421 gcaaaatggg ttagagaaat tttaggggat attcctatat ctttatcaaa tgaaataggt
     3481 aatataggta tattagaaag agaaaatgga gctattttaa attctgcatt gtctcaagta
     3541 ggtaaagctg tatctttagg atttgaaaaa gcacttaaag acttaggtat agatgctcaa
     3601 atatatttca gtcaaaatga tggaacatta atgaatttag agtatacaat gaaataccca
     3661 atttttacaa ttggatgtgg aataacaaat tctataagag gagcttcctt cttatcaaaa
     3721 gtaaaaaatg caatagtatt agatgttggt ggaactacaa gcgacttggg tgtactatat
     3781 aatggattcc ctagagaatc atctattcca gttacaattg gaggagtaca tacaaacttt
     3841 agaatgccag atgttttaac tattggtctt gctggtggta catgtgtaaa aggcgaaaaa
     3901 gataatataa agataggtcc tgaaagtgtg ggatatagaa taactgaaga tgcaataata
     3961 tttggtggag acactctaac tttatcagat gttgttacaa aattagatat ggcgtttgaa
     4021 gatagaaaaa gcaatgttga aaacttagat aaagagtatt gtaatgaggt atataagaag
     4081 gttattttaa aattagaaga tgctgtagac caaatgaaga ctagtcaaga tgatgttgaa
     4141 ttggttttaa ctggtggagg aagtataatt gttcctgaaa aactaaaagg cataagcaat
     4201 gttataagac ctcctcatta tactgctgca aatgctattg gtgcagcttt aggacaaata
     4261 agtggagatg tagaaaaagt atacagttta gataaaatgt cgagagaaga tgccatagaa
     4321 gatgcaaaaa aagaggctgt aaataaagct ctaaaagctg gtgctaaaga agatactata
     4381 gaagtattaa atatagaaga tgttccactt gcataccttc ctggtaatgc acttcttata
     4441 aaagtaaaag ttgttggtga tttaatttaa agaataaata taagtatttc cagaattata
     4501 taaatatttc tggactcatt tttaaactta ttttatcaat aaaaacagca taacttttga
     4561 ctatattctt ataagtttcc gaacagccga tatataccta ttttttgagg tttatcggct
     4621 gtttgttatc ttttatcgca ttccaactat ttttcttacc gtatttgctg tacgaattgt
     4681 aatcttatca cttacatttg aactggcagt ctttgaccac caatttgtct ttttataatc
     4741 tttacgacta aaagaccaaa agatagcatt tttataatga tatacttttt catactcttc
     4801 tttaggattt ccaacctcat cataaaactc gtcataagat aagtcaggaa acataaaaat
     4861 aagattatca tatatttctt tgttgttatc tccccaccaa ttaggagcat tatttagtat
     4921 atcttttaac tcctcttgtg aaataataaa aactggtatt tccaattcaa actgtttttt
     4981 tatcattaat cttattgtat ttgagataac cttgttgtca tctacatcac ttaaaaagac
     5041 aacattacca ctattaaggt atgtagaaac ttctgaataa ccaagttcta caaagcttgc
     5101 ttttaattca gacataatta tcttgttctt accacttata ttgatacctc ttaataatgc
     5161 gatataactt ttcatataat aagttcacct ctaaacattc acatttttct atgtagtaca
     5221 aactggaatt tgcatctttt ataattcttt caaaacttct tcatatgtaa tttttttaat
     5281 ataaaaaaat cggtgcttaa tttgtaccga tttttatttt tcttagtaaa gaactcgttt
     5341 ttttgcttct gctatagctc tagcagcact ttttgcttgc attctttttt cttcttcttt
     5401 tttaaattca tttattgttt catttagatt cttatcatgt tcattttttt ctttatcttc
     5461 gttttctctg tatactaata tataatctat tattgattcg tttattatct gagatatact
     5521 tttattttca ttaattccta ttctcttaac ttccttaagt agagtctcat caatatgaac
     5581 cgttgtttgc tttgtatttt ttgccacctt aatcacctct taaaaaaaat tctaacatta
     5641 aaattttttt tagtcaataa attttaatat taaaattctt taaatgtttt atttgatttt
     5701 ctaaaatttt ctattgtatt tatcatctta ttttttatat tttcacctag tacagaagac
     5761 tttagtctag gattttctat atggtcataa attggttcta aaaattcgac ttcgacagga
     5821 acagagttaa ttttaccaat aggttggtat ccttcataag tatcttttga attttttatt
     5881 acgagtggaa caatagggca ttttgcttta tatgctatct ttaaagctcc acttctaaac
     5941 tccgaaacca atgaatttgg ttctttaatc catgttaaat ctccttctgg aaacactgcc
     6001 atactttgac ctgtaagtat attttgagag gcttgggcta tactctttat accctcacga
     6061 ttgttttttc tgtttacata tacacatctt agtaattttg cccattcttt aaatataggt
     6121 atatttctcc aaactggttc atctgcaatt acacatccaa ttggtctttc tacacttgct
     6181 gttagtatga agctgtcaag catactagaa tgatttacaa caaatagaag tggttctttt
     6241 ggcagtgttt ccttaccaat tatattaagc tctatgttca ctatatctaa agatttttta
     6301 gcttgttttt gcataaattc aaatttttcc tttaagctaa atttatcagg attcttttta
     6361 attttaataa ggctaggtat agaagatgta aaacctataa gttgatatgt agcaaatttt
     6421 aagtaattat tcataaaatt atctccttaa aataaaa
//
LOCUS       DAMKPG010000153         6377 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMKPG010000153 DAMKPG010000000
VERSION     DAMKPG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.159"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            236..1180
                     /locus_tag="PC621_003466"
     CDS             236..1180
                     /locus_tag="PC621_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF4110577.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1202..1828
                     /locus_tag="PC621_003467"
     CDS             1202..1828
                     /locus_tag="PC621_003467"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF4110578.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1862..2911
                     /gene="hisC"
                     /locus_tag="PC621_003468"
     CDS             1862..2911
                     /gene="hisC"
                     /locus_tag="PC621_003468"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF4110579.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2914..3492
                     /gene="hisB"
                     /locus_tag="PC621_003469"
     CDS             2914..3492
                     /gene="hisB"
                     /locus_tag="PC621_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF4110580.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3494..4099
                     /gene="hisH"
                     /locus_tag="PC621_003470"
     CDS             3494..4099
                     /gene="hisH"
                     /locus_tag="PC621_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF4110581.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4096..4818
                     /gene="hisA"
                     /locus_tag="PC621_003471"
     CDS             4096..4818
                     /gene="hisA"
                     /locus_tag="PC621_003471"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF4110582.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4800..5558
                     /gene="hisF"
                     /locus_tag="PC621_003472"
     CDS             4800..5558
                     /gene="hisF"
                     /locus_tag="PC621_003472"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF4110583.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5586..6254
                     /locus_tag="PC621_003473"
     CDS             5586..6254
                     /locus_tag="PC621_003473"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF4110584.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2601 a          540 c         1255 g         1981 t
ORIGIN      
        1 atatagtaaa attaaataca aaattaaatc atcaccatca tcatagatta aagggattgt
       61 aatatgaaat taattcatag acacaagccc tgtttttagt gtaattggtc ttgtatctat
      121 gatgggaagt aattttagga gaaaattata gccatatagg tataaagcct atatggcttt
      181 tttattgttt taaatttgca aatataagag taaatttaat gaagagggtg agaatgtgaa
      241 atttttgagg attgaagatg aagttattta ttctaaaaaa agatataaat taaaaaggga
      301 aatagataaa ctttttttgg atgaagaata ttttcaaata gaaccacaat tatttgaaga
      361 atatgatgaa tttacaacta taaataataa aattccagaa gaatctatgg taaaggttgt
      421 aaatggaaaa gtaatggtac ttagagctga tataactaca agtataataa aaagccttgt
      481 accaagatgg gaagatggtt taaagcttaa gcttttttat aattcatcaa tatataaaaa
      541 taaaaataca gttggaataa aagagataag acaaataggt tgcgagtatc taggagaagc
      601 ctctgtagaa gcagatagag aagtagtgaa attagctctt aagatacttg aaaaatacaa
      661 taataatttt attttagagg ttgggagtag caagtatatt catggtttgt tagaagaatt
      721 aaatttaaat aagaattgtg aaaatcaaat taaaaattta ctatatacga agaacactca
      781 tgaattaaaa gtatatattg aagaactgaa aattaagcgt gaagtaaaag agctattatc
      841 aaatatttta agcttgcaag ggaatttata caacgttatt gaaaaatcaa acaagttcta
      901 ttgtaataat aagatgaagc aagctcttga agaattaaag caagtaaata atttaattga
      961 ggaatgtaat tttttagata aggctagatt tgatttatca atgattacta tgcttgacta
     1021 ttatgaagga attatgttta gaggatacta cccaaactca tataaagaaa ttcttagtgg
     1081 tggaagatat gattctttaa ctaaagagta tggaaaagaa attccagcca ttggatttac
     1141 actaagtgtt gatgagttaa tgaagtatgt atataagtaa agtttagaag gagggattgc
     1201 tttggattat ttgactatag cactagcaaa aggacgaatt gaaggggaat catttaaaaa
     1261 atttaaaaaa atgggtctag gagatagtat agacacagat acgagaaaac taatttttaa
     1321 agatgaagaa aataaaatta tatatataca tgtaaagcca tcagatgtag ttacatatgt
     1381 tgaaaaagga gtcgctgacc ttggtatagc aggtaaagat actattttag agaatgagac
     1441 agatgtatat gaaatatatg atttaggttt tggaaaatgt aaatttgctg tagcaggatt
     1501 aaagggagat agcatatata gagaagatga atatctaaaa gtggcaacaa agtatcccaa
     1561 tatagcaaaa aagtatttta aagagaaagg tcaaaaaatt gaaataataa aattaaatgg
     1621 ttctgtagaa ctagctccaa tagtagggtt gtcagatgta attgtagata tagttgagac
     1681 tggcaataca cttaaagcaa atggtcttga aatattagaa gacatatgta atataagtgc
     1741 tagaattata tcaaatagag ctagctatag atttaaatat gaacaaattc agaatataat
     1801 tcgtttattt gaagaacttg ataattaaaa tataattgaa ctttataagg agggatttaa
     1861 gttgagagaa aaagaaagta taagagagtt aaggggatat gaaccaaatc acgtaaactg
     1921 taaagtaaaa cttgatgcaa atgaaggaag taagaggttg tttaaatacc ttattaaaga
     1981 aatttcagat agtgatatag atttaaattt atatcctgaa gattcttata gtgatttgaa
     2041 agaatctata atagattaca taaatatttc tggagtaaat aagaaaaatc ttctagtagg
     2101 aaacggttca agtgaaataa ttgatttaat aatacatact tttgtagata aagatgaagt
     2161 aatattgagt ttctctccaa gttttagtat gtattctata tatagtcaga taaatgggtc
     2221 taaatttata ggtgttgaaa gtgatgaaaa tttagttata aatatagata atgtgattga
     2281 aaaagtaaag gagaataatc ctaaaatagt aattgtatgt aatcctaata atccaactgg
     2341 aacaatatta aagagggaag agataataaa gcttttagat tcaactaata gtctagtggt
     2401 tttagatgaa gcatatatgg attttggaga agaaagcatg ttaagtgatg tttttaagta
     2461 tgataatcta atagttctta gaactttatc aaaggcattt ggattggctg gaataagaac
     2521 aggatatatg ttatctaata gtagtttaat aaattctgtt gagaaagtaa gacctccata
     2581 taatttaaat tcattatcag actttattgc tacaagagca cttagaaata aagatgtggt
     2641 aaaggcttat ataaaggaag taaaagagga aagagaagtt ttatatgaag aaatgattgg
     2701 tatgggaatt aaagcataca aatcacaagc aaattttata ttattttatt ctgaaatcga
     2761 aaatttatca caaaaactta ttgatagagg agttttaata agaaaatttg gtggaaagtt
     2821 agaaaattat tacagagtta caattggtga taaagaagaa aattctatgt ttgttggagc
     2881 tataagagat attttgaaaa aggagaaata ggtatgagaa tttggaaggt agaaagaaat
     2941 acttttgaaa cacagatttt agtagaatta aatattgatg gttctggtaa agctgaaata
     3001 gatactggaa taggattttt agaccatatg cttaccttaa tgtcatttca tggcaagttc
     3061 gatttaaaag tgatttgtaa gggtgataca tatgtagatg accaccatag tgtagaagat
     3121 ataggtatag ctataggtga agcatttaaa aatgcactag gtgacaaaaa aggaatacga
     3181 agatattcta atatctatat acctatggat gaatctctat caatggtagc aatagatata
     3241 agtaatagac cttatcttgt atttaatgct aaatttgata cccaaatgat tggtagtatg
     3301 agtactcaat gctttaaaga attttttaga gcttttgtaa atgaatcgag agtaacatta
     3361 catatcaatc tcttatatgg ggaaaatgac catcataaga tagaaagtat ttttaaagca
     3421 tttgcaagag ctttaaaaga gggtagtgaa atagtttcta atgagatagc atcttcaaag
     3481 ggggttttgt agaatgaata taatagtaga ctatggtctt ggaaatatag attctgtgtc
     3541 taggggtttt agaaaagctg gaatagaaac taaaatttct agtgatatag atgaaataaa
     3601 acaggcagat tcacttatac ttccaggtgt tggagctttt agagattcta taagtgcctt
     3661 agataaacta ggattgatac caataataaa ggagcatgtt tctaagggta aatttatgat
     3721 aggtatatgt ctaggaatgc aattacttta tgaaaaaagt tatgagtatg gagaatatga
     3781 aggattagga cttataaaag gaagtattga taaactggat ataactttaa aagtgccaca
     3841 tatgggatgg aataatctaa aatttaataa ggcaaatgat gatatactta aatatataaa
     3901 cgaagatgat tatgtatact tcgttcactc atattatgca aattcatcaa atgaagaact
     3961 agttgcattt tcagaatatg aaaagaaaat ccctgctatt gttagaaaag gcaatgtata
     4021 tggtatacaa tttcatcctg aaaaaagtgg agaagtaggg ttaaatatat taagagcata
     4081 tggggagatg ataaaatgat aatatttcca gcaatagata taaaagataa taagtgtgta
     4141 agactcacac aaggagaatt tgacaaagta aatgtatatt atgataatcc attagaagta
     4201 gcatataaat ggaaaaatga aggagcagaa tatatacata tagtagattt aaatggtgct
     4261 agaagtgaat ttggtataaa tacaaaaata attgaggata tagcaaacaa tatagatata
     4321 cctattcaag ttggtggtgg agttagagat aagggaaaag tcaaaagttt aataaatgct
     4381 ggtgttacta gagtaatatt aggtagtata gcgattgaaa atttgaattt ggttgaagaa
     4441 ttggtcaatg aatataaaga gaaaattgta gtttctatag acgctaaaga tggaaaagta
     4501 gcagttaggg gatgggaagt tgtaagcgat gtagactcat taacattatg taaacaactt
     4561 gaaaagatag gtgtacaaac tattgtttat acagatatat caaaagatgg gatgttgcaa
     4621 gggccaaatt ttgacatata tgaaagaata gcaaaggaaa cgtcattaaa tgttattgct
     4681 tctggaggcg taacttctat agaagacgta aaaagactaa aagctatgaa cttatatggt
     4741 gcaataatag gtaaagcgct ttatgataaa aagatagact ttaaggaggc acaacaatta
     4801 tgcttactag gagaataata ccttgtctag atgtgagaaa tggaagagtt gtaaagggaa
     4861 aaaaatttaa agatatagta gatgttgata gtccagaagt acttggaaaa ttttatagtg
     4921 attgtggtgc agatgaactt gtattctatg atataactgc atcaaatgaa gagagaaaga
     4981 catctttaga atttgtgaca aaagttgcag aaaatataaa tataccattt tgtgttggtg
     5041 gaggagtaaa taaattagaa gatttcactg atattttaag aaaaggtgct gataaggttt
     5101 ctataaattc atcagcagtc aaaaatcctg aattaataag ggaagcttca ttaaaatttg
     5161 gagcacaatg tgttgtttta tcgatagatg caaagaaaaa tgaagagggt tcatggagcg
     5221 tatacgtaaa aggtggaaga gaaaaaacta atctggatgc aattgaatgg gctgttaaag
     5281 gtgtagaact tggagcagga gaaattgttg taaatagtat ggacgaagat ggaatgaaaa
     5341 atggatatga tatagaatta ttatcaaaga taacttcttt agtaaatgta cctgtcatag
     5401 catctggagg cgctggaaaa aaggaagatt tttatgaggc agttaataaa tctaatgtag
     5461 atggaatttt agcagcatct gtttttcatt ttggcgagat aaaaataaac gatttaaaga
     5521 aatatttaaa aaacatgggt gtagaagtaa gattataagt taaatactaa aaggagatta
     5581 gaaatatgga taataagtgt aacaatgtat acagtgatga agtagaggaa tttataagaa
     5641 gtataaagtt tgatgataaa gggttagtcc ctgttgttgt tcaagaggta gttagtaaag
     5701 atgtactgat gttagcatat atgaataaag atgctataaa gaaaacctta aaagataaag
     5761 tagcttgcta ttttagtaga agtagacaaa aactttgggt taaaggtgag acatctggta
     5821 atactcaaaa agtagttaag atgtcttatg attgtgatgt agatactata ctactttttg
     5881 ttgaacaaac tggtgtagct tgtcatacag gcaattattc ttgtttttac agagacttgt
     5941 ttgatgatag ttcaaaaatg gagcctgaag tacaaacaaa tatattgaaa gaattatatg
     6001 atttaatcaa tgaaagaaaa aataatccag ttgaaggttc ttatactaat tatctttttg
     6061 aaaaaggaat agataagatt cttaaaaaag taggtgaaga gagtagtgag gtaattatag
     6121 catcaaaaaa taccgataaa agtgaactta tttacgaaat tagcgattta gtgtatcata
     6181 cattggtttt aatgatagaa aaaggtgttg aaattgatga aataaaaaaa gaattactta
     6241 aaagaagaaa atagaatagg atagattaat ggtaataaga aaatataccg cttttaataa
     6301 aaagtggtat attttttatt ttatattttc gatttataga ataatattta aattacttga
     6361 taaaattaac tatatat
//
LOCUS       DAMKPG010000154         6244 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMKPG010000154 DAMKPG010000000
VERSION     DAMKPG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.160"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..114)
                     /locus_tag="PC621_003474"
     CDS             complement(<1..114)
                     /locus_tag="PC621_003474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110585.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRS"
     gene            complement(431..940)
                     /locus_tag="PC621_003475"
     CDS             complement(431..940)
                     /locus_tag="PC621_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF4110586.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(942..1589)
                     /locus_tag="PC621_003476"
     CDS             complement(942..1589)
                     /locus_tag="PC621_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110587.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1603..2775)
                     /locus_tag="PC621_003477"
     CDS             complement(1603..2775)
                     /locus_tag="PC621_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF4110588.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            3043..4419
                     /locus_tag="PC621_003478"
     CDS             3043..4419
                     /locus_tag="PC621_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110589.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(4670..5503)
                     /locus_tag="PC621_003479"
     CDS             complement(4670..5503)
                     /locus_tag="PC621_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF4110590.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(5832..>6244)
                     /locus_tag="PC621_003480"
     CDS             complement(5832..>6244)
                     /locus_tag="PC621_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF4110591.1"
                     /translation="GIDYENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMG
                     ASKKGSYGASIIEKIESIIPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQ
                     QLNNHEESQSHPDEQEIYEASMFAKNMMIKASIV"
BASE COUNT         2167 a         1060 c          673 g         2344 t
ORIGIN      
        1 gcttcttata ttcagcatct cagcaatttt gtatgatgag tatccttctt ttatatataa
       61 atttacagct tttagtttaa gttctttgct atatttttta tgttttttag acatagaaaa
      121 acccccttag agtatacgct ttttagactt attataatag ccttttattt aaatgtctac
      181 tataagggga ttatactaaa atagagataa gcctctcttt cattgttgaa attatgaaaa
      241 tatacttgat ttaataccaa aagagtatct catgaactaa aaagttcatt ttgagatact
      301 ctcttctttt ttaattaata tacatattaa ttactatatt agtttattac tatatatcaa
      361 ttactattca ttaattacta ttcatcaatt cttatatatc atttataatt aggttataat
      421 tttatgttat ttacattaat cccataacag tgagtaaaac tcttactatc aacatcccca
      481 ctattgcatc aaatatagga actgccaaca gtaacattct atacttagta ctggcatttg
      541 ctacaagcac tattctggca taatgaccaa ctagagtacc cattaacata catggcataa
      601 ttaatatggt tgcttgtggt gcagtgatta gaccttgtgc aaataagttt gcagctgtag
      661 ctgctcctgc tgccttagca aagaatgctg ctatcaaaac tacaacagat tccccaggta
      721 gtccaaaaat acccattact ggaccaaaaa tatttcctaa aatatcaact aaaccagtta
      781 attgtaaaaa ctgcactatt acataaccca ttatcatggc tggtaatatc tgttcaacac
      841 ctatgtaaaa accttttttt gctcccccca taaatatctc aacagcagtt ttcttaggtt
      901 tactatttac aattgcattt ccagtatttt gttgactcat actacacagc ctccttagcc
      961 ttattctttt tatttcttac acctatgatt aatctaacta gattagcacc aagtatttta
     1021 cagaatatct ctataaggat tattggacct aatgccaata atgctattgg taaaagtgga
     1081 gcttgagtat ttattgtgtt cagtataaca gctgaaccag catattgata tgctacaaat
     1141 atagttcttt catcatctgt tatttcccca ctttcaaata attctttcgt cattattgag
     1201 gctacatctg aactagtaaa tgaagataca aatgctattc ctgctattcc aggtatacca
     1261 agcagaggtc taagtaatgg attaaatatc tttgatgctg ctttcattcc tcccatagat
     1321 tcaactacat ctataagacc tacagcaaaa cagacagttg gaatcaaagt cagagcaaat
     1381 aagaaaccat cttttgctcc cgttcctcct ttacccataa agtctacacc atctgcaata
     1441 gtaccaaatg aacctgtcag attagcaaaa tctaatgctt taaggaagcc ttgactatct
     1501 ttaaatactc ctgaaaataa tactatcaat acaaataaag ataaataccc tttccatgtt
     1561 accttattat ctccagctgt tacatccata taaaatcccc ctttatacct caaatttatc
     1621 taagtttaaa ataactgtag aaattaattt caccctctca aacatagaat ctactactgc
     1681 atattctctt gatgtgtggt tccattctcc tcttacacca aatgaacaga ttgtaggaac
     1741 gtttgcaata gttaaataac ttgcatctga actacctcca agtctcttag catttaattc
     1801 tccaaatcca ttttctcttg acacttcatt tacaaatgta tgaaatctca taacatcttc
     1861 tgtagtttca aatggcatca tttcacttat aaaattaact tctgtagaag ttccttctat
     1921 gtaagtttct ttacatatct ctctaacttg ttctttaaca ttctccattt cttctacttt
     1981 ttcaaatcta aggtctactt ttatactaca atcttctgga atagaatttt ccactcttcc
     2041 acccttaatc acacttacac ttactgtagt tccaacatct aaattagtca atttttgtat
     2101 gcgtagtatc ttattagcca tttcttctat agcatttctt ccaccttcaa aatcattacc
     2161 tgcatgtgtt tctacacctt ttacatgtat attacatcct attcttcctt ttctacctat
     2221 acataaacta ttatctacta atccagtttc catattaaaa gcacaaagag caccttttgc
     2281 ttccctcaat ataacatctg caccagttga atctttatga cctatctctt catctccaga
     2341 aaatactatt tttattggtc tttctttgta tcctatttta tttaaagctt taataacgta
     2401 taaagagatt attatacccc ccttcatatc taaaacacct gggccatatg cttttccttc
     2461 tattatttta aatggattct ctccaaatgt acctgtttca aagacagtat ccatgtgtcc
     2521 tgaaaaaatt atcggctttt tatctatatt agaaccaagt gtacctatca atgtattacc
     2581 attttcccca acatctacta gttcacaatc caaaccttct ttttcaaatt caagctttaa
     2641 tctctcagct aacttgttta cactttcttt acaatgtgta taactttcta tattaacaat
     2701 ctctttccat aatgaaacta tttcttctct attctcatca acaaacttag aaatttcatt
     2761 taaatatgta ttcataatct tctcctttat tttcaaataa aatgtatttc tttcctaaaa
     2821 tctaagttta ttttaggaaa tcttaaaaga ctagtcctta attacaaaat agcacaatta
     2881 ttcttgccag tcaacatatt tttcagtctt ttttatattt tctgattttt ctttcaatta
     2941 tccttttgta aaataaattg acgcgtttta tctactatta taaaatgagg ttataaattg
     3001 attgattagt tacaatactc tcgaaatttt aaaggaggtt ttatgaaata taaaattggt
     3061 tttttgacaa catcttattt tatgtcagat aagtttaaac aagctcttaa agaattaaat
     3121 gatacttgtg aaattacatt tattgagctt aaaggggaga atccttttaa taatttacct
     3181 aatatatact atgagcaagt agataatttt gatggatttg tatgcagtgg tatagttcca
     3241 tatagtgaac ttataataaa tataaaagac ataaaagttc cattaaattt tttaaagctt
     3301 gatgaaagag atttttataa atacttattc aaattattaa atagccaaaa agatattgac
     3361 tttacaaaat catttatgga ttttttaaga gaggacaaca attattatga tatatattca
     3421 ttaatagact ctaataaatg tccatataca attaaagatt ttaatatccc tcaacctgta
     3481 tatgacttta aaaaattaaa tgataaatta ttggaaatac atttagactt attgcgagat
     3541 aataagatta acctttcttt tactagaaat tacatcatta gttgtgaact taataagctt
     3601 ggatatgatt gtatttacat ggttccatct acagaatcaa tcctgaatac atttaaatct
     3661 ctggtgaatg aaataacatt acaaaactta gacaaaaata aatctgcaac ttgtatagtt
     3721 acagtaaata gttcagagta cataaatgaa gatttgatat ttaaaaatga tgagatacaa
     3781 aatcaaatat ataacactat attaaattct ctatcaagac atggattcta tggagttcag
     3841 gtcaaaaaaa atgatatgaa aatagagata cacactacta aagaaatatt aaacaatatg
     3901 accaataact atacagattt ttttatttca gaagatttaa aagaaattaa atatagctta
     3961 aatataggtt ggggcattgg aaatacaaat attcatgcag agcaaaatgc tcgtcaggct
     4021 aatggtaaat ctgcttcttt gaatggaaac tgtactttta tagtaaatga tactaatgat
     4081 acaataggcc ctttatattc aaataaaaat tctaataata ttgaagatag ctcaatagcc
     4141 aataaagttg cttcttttat acctctatct aatacaaatg tatctaaaat catgtgtatg
     4201 attaatgata gaaattcaaa taatgtgtca gcagaaatat tagcagacta tatgaacata
     4261 actcttagaa gtgctaatag aatattgagt atcttatata aagcaggaat tgcaactatt
     4321 gtaaatacaa aactcgataa tcaaagagga aggccaaaaa aaatatataa agttgatttc
     4381 ttatcttttc taaataagat gcagaaatta aattcttaat atttaatttt ttaataaaag
     4441 caatgtattt tctctaattt taaagaatca aaatgtttaa attttattag aatatacaac
     4501 tttttacttt aaaataccta aaccttttat cagtgaaata aaccataata tatcgtagct
     4561 tattagaaaa acaaaataat ttcatataca aataataaaa tagccactac aatccataat
     4621 ttgcagtggc tactttttac actatcactt attacttaat ttttaaaaat taattgtatt
     4681 ttttaaataa tttatacaca cctattatca gtaaaacaat aaatacaagt atatctataa
     4741 aaataattat tgtaacatgt ctggcaatat tgtaaataaa aagtatagag aatatttcta
     4801 tagacaacgc tacaaaacac ataaaattcg ttaaataatc tgcaatagta tcactataaa
     4861 aagcatgtat ttgtaaccat ctccatataa atggacgtaa aaggattctg aatacaacac
     4921 aaatgacagg gtaggcataa ataaattttt tatctacata attagctaca ccattattcc
     4981 attggatagg aatttgaggt ggcaactttg gaaatactgt tattgcaatc aacaaacaag
     5041 caatagtaat aattccccag gtaattttcc ctccccatat atataagaaa cttacagagt
     5101 caagtttaaa aacaggtttg tttttattcc agtattctgg caactcagtt atgtattgct
     5161 ctactttcaa attcaaataa cttagctcat cagattctaa cattttatta cacatagttt
     5221 ttgcattttc taagtctgat attttagttt cgagaacttt ctcctgtttc tctacaactt
     5281 caactaaaga tactttctgt aatataatat tacggatatc atcaatagta attcctagtg
     5341 tacgtaaata tgcaattttt ttgatgtcct ctacattttc atctgaatat tctctatagc
     5401 cattacgctc attacgaagg ggtttaatta aattctcgtt ctcataaaaa cgaatattag
     5461 agcgtgtcaa tcctgttttt tcttcaactt ctttaattgt cattttaaac actacctctc
     5521 tattctatta atttacaact ataatatagt cttaaatatt ttcactctat aaaattgtat
     5581 atttagtata tcttaaaatc aagttaatta tcttttataa attttcatac aataatatct
     5641 cctttcaata atatctcttt ttctatattt aaaactatct tattttctat aaaataatgt
     5701 ataaacaagg tttacagtca agtattttta ttattttttt gatttcataa aaaaaagcat
     5761 agcaaatcat ttgctatact tttttactaa attatttatt ctatatctta tatgaactta
     5821 ttgtttaact actacacaat actagcttta atcatcatat tttttgcaaa catactggct
     5881 tcatatattt cttgttcatc tggatgactt tgagattcct catggttatt taattgttgg
     5941 cgaatatgtt cgttgtcagg agaaagtttt aacatctctt tatattttgc tttcaatcct
     6001 tctgctatct taccttgaca tatgaatgaa cctaatattt cattatcttt tgggataatt
     6061 gattctatct tttctattat actagctcca taacttcctt ttttacttgc tcccattgtt
     6121 ccaaatagtg ctatcttttt attgtgaata ttttcaagca cttgttttga atttttatca
     6181 caaattccct tgtccaccca atagcctaag aatactatat cataattttc atagtcaata
     6241 ccat
//
LOCUS       DAMKPG010000155         6165 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMKPG010000155 DAMKPG010000000
VERSION     DAMKPG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.161"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            174..3353
                     /locus_tag="PC621_003481"
     CDS             174..3353
                     /locus_tag="PC621_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4110592.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIYKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            complement(3638..4402)
                     /locus_tag="PC621_003482"
     CDS             complement(3638..4402)
                     /locus_tag="PC621_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110593.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4639..6132)
                     /locus_tag="PC621_003483"
     CDS             complement(4639..6132)
                     /locus_tag="PC621_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4110594.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2309 a          845 c          718 g         2293 t
ORIGIN      
        1 ttcattcata gtttataaga taattcaata aaaatctaat agtaaattgt tatttacaca
       61 tatattgtaa tagataaaag ctattcatat ctgccttaat tttagtctta aatttagagt
      121 gtaaattgaa tgcatttaga ttataatata tacagtgagg taaggtgaat aatatgaaaa
      181 tagatatgat atataaacta ataagaagaa atacagaacc atcaagatgg tcaagaggtg
      241 taaaatatta tactaatcaa ttagttgatt atatagacat agatattgat gacctacaaa
      301 ataacctgtt gacatttcgt ggtggagtca attctgaaga taattatagc ttttatttta
      361 attcaataac tatagatata aagcacttta agataattga aaatgattgt agttgcgctg
      421 actgtcaatc aaagggcaaa agtattttta tatgtaagca tatagttgct attgtgataa
      481 aaagcttaaa acaaataaaa aataaaagag atgttcctcc tattctaata gatatattag
      541 gtgcagataa ggataagctt tctaatacaa attctaaaac aagcttagat ttaagcaagc
      601 aattactttc tttgttagac aatactaaaa gagaaagggt taatttagat gtattgataa
      661 atgtggattc aaataaagca attgctgaat ttaaaatagg aattgataaa atgtacattt
      721 taaaaaacat tactgaattt gcatattcaa aacttaatgg atatgaatta gattttggga
      781 agaaatttac ttacaacccc aacattcatt attttgctga tgaagatgag aagttagtag
      841 atattataga agaatatggg acaaatcttt catacaactt ttcttcatca cattcaaaaa
      901 aatttatgga gataaatgca agctctttaa aacaattttt aaatgctatt aagtacaagg
      961 attttaccct tattttttct aaagaggaat atcatccaaa gatattaaat gatactctac
     1021 ctgtaaggtt tgattttaaa aatatagaag ataaaatagt cttatattct aaagattcac
     1081 tacctgttcc acttactaaa aaaaatgatg tacttttata tgatggcaat atctatttat
     1141 tatcacataa aaaagctcat gattattcaa aaatatatca aatattatca aagactaaag
     1201 agttaaaatt tgataaagag gattctaagg atgttttagg attgctagtt cctagattaa
     1261 aatctatatc tcaagaagtt catttagatg ataacataaa aaataatata acaaaagatt
     1321 ttaaatctga gttctacttt gatatgattg atggcaacat atgttgtgat gtaaagtata
     1381 tatatgatga tgaggataaa aaatttgtgc tccccaacat acaaaaggaa actactattg
     1441 aaaataaact tacttcttca caatttacaa aggaaaataa tcattatgtg tttaaaggaa
     1501 ctgactatga cctatttaca tttttagatt tacaattaga atctctaaag gaatttggag
     1561 atatatatta ttctgagaag tttaaattga aaaaaatata taacgcttca tcaattcaag
     1621 cttcaatcaa tcagactaac cagaattacc tagaatttaa ttttaatata tctgatataa
     1681 atccaaaaga gtacaaggat attttgaaag cttttcaaga aaaacgtact ttttataaac
     1741 ttaaagatgg aaactttata gatttatcag aaagagaaac aaaagatttt tttgaattag
     1801 ttgaaaatct agatataatg gataaatcaa aaaataataa aatttataaa aataaagctc
     1861 tatatataaa tgatgtaata cgtagtaaaa atttaaagtt tatagatggt aaaaaagaac
     1921 tagacagtat atgtgaaaaa tttagaaatt ttgatagcat aaatttagaa attcccgcaa
     1981 atttaaatgc aaaccttcgt gattatcaaa taaatggcct caattggttt aaagttttag
     2041 attattataa atttggtggt atattagctg atgaaatggg tcttggtaaa acaatacaga
     2101 ctattgcatt tttattatca ttatctaata aaaaaagtct tattgttaca ccgacatcac
     2161 tcatttataa ttggaaaaat gaatttgaaa agtttgctcc agatataaaa gttttattga
     2221 tacatgggaa taaaagagat agagaaaaat gttttatgga attagaaaat tttgatgtaa
     2281 tacttacaac atatggtact cttagaaatg atttagaaaa atatagtgaa ataaagtttg
     2341 attattgtat attagatgaa gctcaaaata taaaaaatcc agtagcttta gtaactgagt
     2401 ctgtaaaaag tataaatgca gaaaataaat ttgcactcac tggtacacct atggaaaata
     2461 atctactaga attatggtct atatttgatt ttataatgcc tggctatcta tatagtaaag
     2521 caaagtttca ggaattgttt ataaataaag aggataatgt taaaaacctg aaaaaattaa
     2581 taaaaccttt tattcttaga agaagtaaaa aacaagttat gaaagaactt ccagataaga
     2641 ttgaaaaaaa cttttttgtt gaattgaata aagagcagaa aaagatatat agtgtatatt
     2701 caaaagatat acaagataaa atgaaagata aaaatctaaa gaaagataaa atagttatat
     2761 tttcttattt aactaaacta agacaacttt gtttagaccc aagtatagta gtaaaagatt
     2821 acaataaaaa aagttcaaaa attgaaacat gtttagaaat acttagggat agtataaacg
     2881 aaaaccacaa aatattattg ttttcacaat ttacttctgt gcttaagaac ataagtaagg
     2941 aacttgataa gtataaaatt aaataccatt atatagatgg taaaactaat gccaaagaaa
     3001 gacttgaact tgtagatgag ttcaataata gtatggataa aaaagttttt ttaatatctt
     3061 taaaagcagg cggaactggt ttaaatctta catctgctga catggtaatt cactttgacc
     3121 catggtggaa tccatcagta gaaaatcaag ctagtgatag agctcataga tttggtcaaa
     3181 aaaattcagt acaagttata aaattgattg ctaaagggac tatagaagaa aaaataataa
     3241 aattacaaga aagtaaaaaa gagcttataa atcaatttat aaatggagaa ctttctaatg
     3301 aaggtgtatt aaaaagtcta tctgatgaag agataataga cttatttaac taaattattt
     3361 aatcctacag cttaactata taaatttatt ctaacattta tatagttaag ctacaatatc
     3421 tgaataaata aaatttcata ttgttattta atcccaactt tacaatctaa tgatatctct
     3481 ttctatacat attgcatata caaatgtaat aacttgacta ttagaatatt ttgttttaac
     3541 aatcctattt catcataata atgaagtata tgtacactca cttttgtaag ttttgcaatc
     3601 cattttacag ttttcattat tgtaaatttt ggttaaatta ttcctctaaa aatttctttg
     3661 ctacatttga aaaatctaat ccattcgata aaattgcagg atgaccacct ttatcaaaca
     3721 aatacatttt accattttta atcttttcaa gaagtaatga aaaagtattt tcaaaaaaat
     3781 cactagatat tagagataca aactcatctt ctctacttcc tgttaaaagc acctctggtt
     3841 gcatagtttc tagaggctta tgaaaaaatt ttcctatagt tttataatgt tcaaaaatag
     3901 cttctgtatc attatctaca actttttccc agttttctcc ttgattataa atataaaaag
     3961 cttttgcacc atcatcttgc tttgatgcct cacgctctat tttaacattt tgtacaaatg
     4021 actctaaagg aacttctcct tcaaaactat ctgcaattac cttattcact aaatcaggtc
     4081 tctcaagtgc aacatttaat gcaacaagag ctcctccact actaccaatt atgtttactt
     4141 ttttatagtt cattgcttct aaaaaacata tcacttgcat agcctcatca aaccataagt
     4201 cagcagaaaa tttatcgacc atttgagatt ttccatgacc tacaaaatca attaatatta
     4261 ctttatattc atctttataa aaatcgatta tatcattaaa cattttggaa gaggctgtat
     4321 ttccatgtaa aaaaagtaat ggtgtaccat ccccaatttc attatagtag cactgtttgt
     4381 cgttatagtt aaaatatgcc atatattacc tcctttaata tccctatagg tatatattta
     4441 ttacttatat ttaaaatgac tattgtcata aacatttaaa aatagtatag cttagaatgt
     4501 agataaagtc aacattcctt tccttaacaa cttctattgt tttttgaaac ttcatttcga
     4561 taacagaata attcataaat attgatactc ttacttaaat atatgatttc atcatcagag
     4621 agagcagtaa taactaaatt atcgtatatc tcttttcact ttaaccattt ttttctttat
     4681 aggtatctcc ttatattttt ttaatatcaa atctccctta taatttaata tatctacata
     4741 tgaacataaa tcttgaactt caattacacc tatatcatca ttagttatac cttttatatt
     4801 actaaatgca ccaactatat ctaaaactct tattttcttc ttcttaccag catttaaata
     4861 taatttaaca acttcacttt ctatatttag ttttatatct ttacttttgt aagaattttt
     4921 atcaactttt tttctctttg atactttttt taggattcct tttgaatatt cttcaaacaa
     4981 aattttgcca tgtgtaattc tacttttttc tatatctttt aattcattta ctttatatcc
     5041 tatatacgtt tctatattct ctatatattt ttcatccttt tcagttacta tagtaattgc
     5101 ctttccacta tttccttttc tcccagttct acctattcta tgtatatagt tttctttatc
     5161 ttgtggcaca tcataattta ttactaaaga aatatcatca atatgtattc ctctggacgc
     5221 tacatcacta ctaactaata tattaaattt ttggttttta aaatccttaa ttacaaaaat
     5281 tcttctttct tgtgataaat ctgcatgtaa ctctcttatt aaaaaaccct ctttactcat
     5341 cttattatat aatttagata ctttctcttt ggtattacaa aatattatca cactctttgg
     5401 attttcgctg tatattaatt cttttaaaag gatgtacttc tctcttccat ttgccttgat
     5461 tattttgtca tctatttgtc ttgttttttg attagtatcg ctctcatttt cttctatatt
     5521 tataacactg taatctagca tatatttttc acatatatat tttatctctt catctattgt
     5581 agctgaaaaa agacttacta ttttctcctt aggtaaattt aataatattt tttccatttg
     5641 ttcaacaaaa cctttattaa acattttatc tgcctcatct attactaaat actttacatt
     5701 ttccaactta atagaacctt tatttatatg gtcaagtatt ctgcctggag tagctacaac
     5761 aatatgaacc ctttgtttca attcagctat ttggtctttt atagattgtt tgccaaatat
     5821 agcactacat ctcaccttct ttagtctccc tatatctgat atctcatctt ttacttgcaa
     5881 ggctaattct cttgttggaa ccactataag agcttgaata ttattgcaat ctacatttat
     5941 attctcgcat aaaggtattg caaaacttgc tgtctttcca cttcctgttt ttgacctaac
     6001 tactaagttt tgacctttta ataattttgg aataacttct ctttgtactc tagaaggtat
     6061 attatatcct aaactcttca atgacttaag tatcttttca tttaacttaa atttttcaaa
     6121 tgtatatttc atatattacc accttattat atttatctta tctat
//
LOCUS       DAMKPG010000156         5937 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMKPG010000156 DAMKPG010000000
VERSION     DAMKPG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.162"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            114..680
                     /locus_tag="PC621_003484"
     CDS             114..680
                     /locus_tag="PC621_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4110595.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            720..1301
                     /locus_tag="PC621_003485"
     CDS             720..1301
                     /locus_tag="PC621_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4110596.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1358..2275)
                     /locus_tag="PC621_003486"
     CDS             complement(1358..2275)
                     /locus_tag="PC621_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4110597.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2405..4036
                     /locus_tag="PC621_003487"
     CDS             2405..4036
                     /locus_tag="PC621_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF4110598.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4039..4986
                     /locus_tag="PC621_003488"
     CDS             4039..4986
                     /locus_tag="PC621_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF4110599.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5271..5534)
                     /locus_tag="PC621_003489"
     CDS             complement(5271..5534)
                     /locus_tag="PC621_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF4110600.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5633..5878)
                     /locus_tag="PC621_003490"
     CDS             complement(5633..5878)
                     /locus_tag="PC621_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110601.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         2135 a          815 c          985 g         2002 t
ORIGIN      
        1 aaataattta taaatatagc caacttagcc taaatacgta tataatatag ctaatatggt
       61 tcttatactt gataccatca ataaaactaa aaatataaaa aggagaactt aaaatgagtg
      121 ttagtttaca aaaaggacaa agagttagct tagtaaaaga taataatcct gtaaaaaatc
      181 tagtagtagg cttaggatgg gatatgaata aattgggtaa aaaaaattat gacctagatg
      241 catttgcaat agcattgact aaccaagata aaatgaatgt tagaggtgac ttggtttatt
      301 ttggaaatct taaacaccct tctaaagcta ttatacacac tggagataac ttaactggaa
      361 aaggtgaagg tgatgatgaa caaataattg ttaatttaga agacatacct gaatatgtac
      421 ataaaatagt ttttggagtt tctatataca aagctaaaaa aagagaccaa gattttggtc
      481 aaatgaacaa ctcttttata agattaattg attcaaattc caagcaagaa ttatttaaat
      541 ataatcttca agaagacttt tctggtaaag taacagtttt ggcaggagaa atttatcgta
      601 gaaatgaaga atggaaattt aatgctttag gaattggtca aaatgaagag cttcgtgaat
      661 taattaatac atataagtaa taattacaat aaaaaattta taatgaggag ttggtttaaa
      721 tgggtataac tttagcaaaa ggacaaaaag taagtttaac aaaaagtaac cctggtctaa
      781 aaaaagtaat agtaggacta ggatgggata ttaataaata tgatggtgga tttgatttcg
      841 atttagatgc ctctgcattt ttaactggaa ctgatggaaa agtaacgaat gatggagact
      901 ttatattcta taataattta aaacacgctt ctggagctgt tgaatatatg ggtgataata
      961 gaacaggtgt tggtgatggt gatgatgaac aaataaatgt agacttatct aaaatacctc
     1021 aaaatattgc taaaatatca tttagtgtta ctataaatga agcaattaca agaagacaaa
     1081 atttcggaca agttgaaaat tcatatataa gaatatataa tgaagaaact aacgaggaac
     1141 ttattaaata tgaattgggt gaagatttta gcatagaaac agcaatagtc gtggcagaac
     1201 tatatagaca taatggagag tggaaattta atgctttagg ttctggattt gaggatggac
     1261 ttgcaggatt gtgtaaaaat tttggcgtta atataggcta atatatctaa ataaaaataa
     1321 gagtatattg atatactctt atttttattt agatatatta taaactgttt acacttctac
     1381 tcttctttct cgcttgtttt ttcccatatt ctactgaaat atcaaaaaat gctgaaatat
     1441 tttctgtaaa aaacttgctt ctatgatatg caatatcgaa tgttctatgt aagttaatac
     1501 cttcaatatc acaagcccac aaaagatgct tttttaaata atcctcaata agtaattcgg
     1561 atataactgc aatgccaaaa ttatctacaa ctgctcttaa tatagcttca gagttataac
     1621 atgaccatct aatattcatt gggattttta attcttttaa ttgcttttct aattgtgctc
     1681 ttgtaccact tcctaattca cgcataatta atggttgatt tgaaagttct gaaactttta
     1741 tagagtctct tttgtagaac tcatgcttgt gtgaacatat aagtacaaga ttatcattaa
     1801 taatgctttt tgtaacaata tctgggtgct taatttttcc ttctacaatc gcaatatcta
     1861 attcactatt aagtaatttt tttgcaatca gtcttgtatc ttctacaaat acttcaggtt
     1921 ctataagagg atttaccttg tataattcta acattatcgg gcttattaca catgtaccaa
     1981 cagttactgt tgccccaact cttatacgga tattttttgc acaatgcttc atacaatcat
     2041 tcatttcttc atttacagct aaaagatgcc tcccatatcc aagaagtttt ttccctgcct
     2101 cagttaaata taattttttt gaaattctat caaataactt aacaccatac tctttttcta
     2161 tttctgaaat agcttggcta actgatgact gggtaatata catatttctt gctacttcac
     2221 tcattttacc acattcagca accttaacaa agatttcaag actacgaatt gtcatattat
     2281 cacaacctta gcaaacatta gtaaatgctt atgattttat taatatttta gtattttaca
     2341 aatatattgt caagaagtat aattttatta tagagttata aagtagattg gagtgtgata
     2401 tattgtgaaa gtattaatta taggtggagt tgctgctgga acaaaaactg ctgcaaaaat
     2461 aaaacgtgaa atgggtgata attgctctgt tacaatacta aataaaggtg atgatatttc
     2521 ttatgcaggt tgtggtcttc catattacgt tggtaagata attgaagata aatcatcttt
     2581 aatcgtaaat acacctgaaa gtttttctaa actaacagga gcagaagttc gttgtggagt
     2641 tgaagttacg tctattgata gaaatgcaaa acaagtaact ttgaaaacgc tttcaacagg
     2701 agaaaaagaa aatctaagct atgacaaact tgtaattgct acaggggcag acccaataaa
     2761 accaccaata cctggaatag atttacctgg agtattcttt atgcgtactc caaatgatgc
     2821 tataaaacta agagattcta ttgaaaacgg tattaagaga gctgttgtta ttggtggagg
     2881 tttcattggt cttgaaattg ctgaaaatct ttctgcaatg ggtatacgtg taagtgttgt
     2941 tgatatggct gaacatgtta tgcctggatt tgatactgat tttgctgaat acgttgaaaa
     3001 tcatatggct gataaaggca taatgatttt tacaggtgac caggttgtag gtattgaagg
     3061 tgaaaataaa gttgaaaaac ttcgtactaa aaatagagct attaaaacag atttagtggt
     3121 tatgtcagtt ggaatccgtc ctaacacagg ttttctaaaa gacacaggtt tagaatttgc
     3181 ccctaataat acaattattg cagatgaata tatgcttaca aatgataaag atatatacgt
     3241 agttggagac tgtgcttttg taaaaaattc tttaacaaat aaacctacat ggtcacctat
     3301 ggggtcatct gcaaatcatg agggtagaat ttgcgcacaa aatatatctg gaaaatctaa
     3361 aacttacaat ggtgttcttg gaacgacagt agtaaaatta cctgagctca atgcaggaaa
     3421 aacaggtcta agtaaagaaa tagctgaaaa agaaggttat aatgtttgct ctgtaaccat
     3481 agctaccgat gataaagctc attattatcc aggtgcatct aattttatta taagaatggt
     3541 tgctgaaaaa aatactagaa aattattagg tgttcaagtt atgggacctg gagcggttga
     3601 taaaatagta gatatcgctg ctactgcaat aactttaggc gctgaccttt attcattaga
     3661 aagtatggac cttgcatatg caccaccttt ttcaacagct attcaccctt ttgtagtggc
     3721 agtaaatgtt ttacaaaata aacttaacgg agaacttgat agtgttttac ttgacgaaat
     3781 tgaggatttt gacagttgga ctaaacttga tgtttcaaaa gctccttcta ttcctagttt
     3841 acgttatatt cctgttggtg aaattaatgg agaaattgaa ggtcttgcta aggatgaaaa
     3901 aattcttctt atatgtgcta aaggtaaaaa tagctatatg gcgcaaaatc gcttaagacg
     3961 ctttggctat acaaatacga aagttcttga aggtggaata ctattttatc caaatcttaa
     4021 ggtaatggag gagtaaatat gagtaatgtt acagcagaac aaaaaaagga agtaaaaggt
     4081 agaggttttc taccaagcag ggatggagaa cattttgcag cacgtataat aactgtaaat
     4141 ggtgtaataa atgcatcaca agcaaaaaaa attgcagaag ctgctgaaaa attcggaaac
     4201 ggtcagttag catttactac tagactaaca gtagaattac ctggtgtaaa atttgaagat
     4261 attgaagctc taagtgaatt tattgctagc gaaaatctaa taacaggtgg aactggatca
     4321 agggttcgtc ctgttgtggc ttgtaaaggt actgtttgtg ttcatggtct agctgataca
     4381 caggcacttg ctactgaaat acacgaagaa ttttacaaaa attggtatga tgtaaaacta
     4441 cctcataagt tcaaaattgg tgttggtgga tgccctaata actgtattaa acctgaccta
     4501 aacgatcttg gaattgttgg tcaacgtgta cctgactatg attcagagct ttgtgttggt
     4561 tgtaaaaaat gtgctgttgt agaggtctgt cctgttaaag cagctaaatt aacagataaa
     4621 ggtaaacttg aaatagacag taatctatgt aataactgtg gtaagtgtat agaaagttgt
     4681 aattttgata gtattgaaga aaaagaatct ggttataaag tctatatagg agggaaatgg
     4741 ggaaaatcag ttcgtcctgg aacacagatt gatagattat tttcaaaaga ggaacttatg
     4801 actttaattg aaaaagcaat tcttttattt agagaacagg gtaacacagg tgaacgtttt
     4861 ggaataacta tcgatagaat tggtgctgat aaatttatag aaatgctttt atcagatgaa
     4921 gttttggaaa gaaaagaaga gatactagag gctcttttac accttacagg tggagctgta
     4981 tgctaacaat aaatatttta attgtacaga taacctatgg aaaaaatttt agttaagaat
     5041 aattaaaatt ctatatatga tatatgataa atgaggctat ctcaaaacac agatacgcct
     5101 ctttttcatt gttgaaatga taaagtatat ttgagtttat acaaaaaaga gtgtctcgtg
     5161 aattataaaa ttcattttga ggcacccaca ttattttata ataattgctt attaatttta
     5221 ttttaactat ataaaaagta caaaaagtaa gaatatccaa tattctaaaa ttaattttga
     5281 attgtattga ttgaaaagtt tactattcca ctatttccat cttgatttac tactagatac
     5341 catgttccag tattaggaac tctcattcta catggatttt tctttgcaaa tccaccatag
     5401 taatcataat ttctttgatt tttaaatttc acataattta tatggtctaa caatattaca
     5461 ttggcttgtt tctctaaatt tacctctaca atatcccctt tttttaagtt catcttacta
     5521 taactatatt ccattgaatt actcctccag tatcgaataa tctttgttat ttactcatct
     5581 caaatatgta aaaattcatt taaaatatta caaatactat ataattagac tattatccag
     5641 ttttaacctt atttactaca atccaaaggt tattaaaaat attattggca acaacttctc
     5701 catcccatac aactctttct tctttatttt ctaataaagc atagtaccca gagtcagaac
     5761 tttcatcttt atagactgta acataaaact cttcatcact atattcaggt atatttttaa
     5821 agtcttcaga tattttacat gttaatatat acccttctaa ttcttcatct ataaacaaag
     5881 caattcctcc aatctctata tttttactaa ttataccata ttatgtcata aactttt
//
LOCUS       DAMKPG010000157         5708 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMKPG010000157 DAMKPG010000000
VERSION     DAMKPG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.163"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            230..412
                     /locus_tag="PC621_003491"
     CDS             230..412
                     /locus_tag="PC621_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110602.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1244..2158
                     /locus_tag="PC621_003492"
     CDS             1244..2158
                     /locus_tag="PC621_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF4110603.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2548..3195
                     /locus_tag="PC621_003493"
     CDS             2548..3195
                     /locus_tag="PC621_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF4110604.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3362..3931)
                     /locus_tag="PC621_003494"
     CDS             complement(3362..3931)
                     /locus_tag="PC621_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110605.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3936..4835)
                     /locus_tag="PC621_003495"
     CDS             complement(3936..4835)
                     /locus_tag="PC621_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF4110606.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4846..4995)
                     /locus_tag="PC621_003496"
     CDS             complement(4846..4995)
                     /locus_tag="PC621_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110607.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4988..5572)
                     /locus_tag="PC621_003497"
     CDS             complement(4988..5572)
                     /locus_tag="PC621_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF4110608.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
BASE COUNT         2092 a          860 c          661 g         2095 t
ORIGIN      
        1 atttatatta aatatctact aactcaaata ttcataatta aactttaaac tggataaatt
       61 ttcttttaag aatgaaagtt tatccagttt tgcgattata ataaccagtt ttacatatcc
      121 acttgtaaaa tatatctaag caatatatca tactatatag atttatgagt gaaataatat
      181 ctgctattta ctgaatattt ttatttttcg ttttttaggg ggattagttg tgtttaaaga
      241 taagattgat gaatgtgttc atataatgac tgcatatatt gctagtttaa aagaatacta
      301 ttcattcata gaaactcaaa ttggtgattt tattaaaaaa tatggagaag atgttgtaga
      361 gttatgtctc catagagtga tgattctact atgcgaatgt ggtttggcat aaaattaaat
      421 ttattaaaat gggtaagaag aatcctatat aatattcttc ttacccattt taatatatca
      481 atttcatagc ttatcgttta aaatatttta cctccattac tagacaactt tacattctct
      541 ttttaattct agtatattta aatcatttta gttgctcctt atttattctt attacttgtt
      601 ttattttata aataaaataa ttctttttaa taatgttttt tgttgatatt cctatcacac
      661 atccaattat ttatcattgt cttgaaattg tttgcatact taatttaatc caattgaaac
      721 accccagcat agaatcccct gtaaaataga agttggatta aatcctaaca tccatacaga
      781 aaatactact ccaagcacaa gcaatataat ggaatatatt tatcacttaa ttttttatac
      841 tttttaaatc ccatacctaa aacattaagt gcagctatta gaattaataa ttgttgtggt
      901 ataaaattat gattgcttca tatttattca ttttttgcta taaaagttct tgtttcacaa
      961 aaaatatttg ttttgtatca aaataatact tataaattac ataattctca taaaaatttc
     1021 tagtattact tgtgctctta ataaataata gccaacaagt accttaaatc attatatcta
     1081 tatctaataa gaaacacagt aagataattt tttcgcccat aacttaataa aaactgttga
     1141 tattagtagc aattgaattt agttaattta tttataatat acataaattt aaatattacg
     1201 aataaagtat tataaatagg atactttcta aagggaggaa attatgatag ataatcaaaa
     1261 atatgttatt ttatcactag aattacattt atttttttca agaattatga aagagcatgc
     1321 tcttttttta gaagcaggat tcacaaataa aaattataat cttgctatgg aagctgacca
     1381 ctataaaaag caatttgaag atttattatc atacactgtt agtgctagta atggtataat
     1441 tagacctgat atattatatt cagaagagct tgtaactact ctcacatcag ttgcagaaca
     1501 aaaaacagaa gagtttacag ggatagaaat aaacaaagac atcactacaa gagaattaaa
     1561 tctacaaagt ggtgtaaacc cacaagttgg tcaagactta gtgaactatg tagctcaact
     1621 taactctgat gcaataagat tacttgatgg gcttattaat tttaaagaaa gagtcttaga
     1681 tggtgtacta tcatgtacta tatttacatc aaactaccct ctacttcttg aacatataat
     1741 acatgaagca aatttatatc gttcttatgt agttgacctt gaaaataaaa tagatattga
     1801 gtcaaaaaac gctaaagaaa tagaattatt ctgggaccat attatgatgg aacatgctct
     1861 gtttatgaga ggattactag acccctcaga aggtgaacta ataaatactt caaatgattt
     1921 tgctataaaa tttaatgaat taattgaaaa aacaaacgaa atgactgatt ctaatatcaa
     1981 gaacattaca gaagaaactc taaatgaaac tgttgagttt aaagatttta aagaagcagg
     2041 agcatcagga atagaacagt gtaagataaa atctataata ttaccacttt tagcagacca
     2101 tgttttaaga gaggcaaatc attatattag aatattggag agttataaaa acatgtaatt
     2161 aaaaattact tttaaaataa tctttgcata tttagataaa ataaaggtac agttaactgt
     2221 acctttattt tttataaact tataaaattt aaacaagtta ttcataaaat aaaataatca
     2281 tcatctataa cccacaactt tattttatat taaataaaaa gtattagtga ttatgtttta
     2341 taagtatttc tttattcagt tatttgttaa aaactaatag taaatcttat actaatcgca
     2401 tatttatgtt ataactatat tgtaactatt ttcgtaatct taggaaaaga ttacttataa
     2461 aattaacctt atacaataat atatcgtata atcttaatta gcataatagt tacaatatta
     2521 aaatttaaat atgggagtga ttttagtatg ataagacaaa caccattaga gaaacgatat
     2581 gacaagctag gtcctcatat tgtaacagca ttcaaaaaac gttattttga tgcttattac
     2641 tgtaaaacta gaaatgaagc tttgcaacaa attttagatt taattcctca aaatcatgta
     2701 gtatcatggg gaggatctga aacacttaaa gaaatgggcg tacaaactgc tgttagagag
     2761 aaaggattta aagttattga tagagattta gcaaaaactc cagaagaaag aactgaaatt
     2821 atgcgtcaag cacttctatg cgatactttt ttgatgagta gcaatgcaat tagtgaagat
     2881 ggacaactct ttaatattga tggaaatggc aatcgtgtag ctgcaatgat atttggtcca
     2941 aaaaatgtta tagttgtagc tggtatgaat aaaattgtaa aaaacattga tgatgcaata
     3001 caaagaacta gaacaattgc atctccagca gttgttcagc gttttcaaga tgtcaataca
     3061 ccatgctata ttaatggaag ttgtatagat tgcacaagtc ctgaaagcat ttgtgcatac
     3121 atggttacta caagaatcag tcgtccagca aaaaaaataa aggtaatatt ggtaggagaa
     3181 aatcttggtc tataaaatat acatacatgt aaatagctct ataaataata aaatcatatg
     3241 aaataactta tttctaaaag tggtagaata aaagcttcta caagactttc attctaccac
     3301 ttttatattc ttttaatttg tgtttctata ttattaattg ctccaatttt catatatatt
     3361 actaactatt tttattttca taattttttt gttcttcatc aagctgttta taataatctt
     3421 tattatagtc agctagataa tctataccac ctcctgtaat cacatttaat aatttcattg
     3481 cagattcttg attaacttta caaacataat tatcaccaga tttaacaagc tctaatgtaa
     3541 tattaaattc ttttttcttt gcagaatcaa tatctttcat agttggattt ggcatcattt
     3601 ctcctagttt aggaggattc ttttttaaaa tagaaaccat atcagtattg ttaattttta
     3661 aatctacagt cactttatcc ttattattct ttatatctga aatttcccaa gtcatatttt
     3721 tagaatacat tttaaatcct tctttatctt cgtatataag attacctaac cctgattttg
     3781 ggaaatactt atcaacagta tctgcatctt gtgtttttat tgaattaaac atactatcca
     3841 cttcattttt tatcttatta tcattagtac aaccagctaa caccagtact gatattgcaa
     3901 acattataat acatagaata aatttcttca ttaaattata tccccctata cttttttgca
     3961 caaatattat tctctttttt atcttttgaa aaagttgtag taattgcatt ttgtggacaa
     4021 acttttatac attcaccaca cctaatacag tctggactat ttacatcttt atatattggt
     4081 atatcaagct tacattttgt ttgacatgct gtacaatttg tacatttatc tttatcaatt
     4141 ttaaatctat atattgaaat aggattaaaa aaaccataga ttgctcctaa agggcatata
     4201 tacttgcaaa atggcctata agtaatgatt gacatcaata atatcaatag caaaatactt
     4261 aacttccagc taaataaaaa tcctattgat tctcttaaat tctcatttcc taaaataagt
     4321 ggtattcctc ccaaaagtgt tcctgaaggg caaatccatt tacaaaacca aggatttcca
     4381 ccccctgcta catttacaat agcaactggc aaaataatta caaacattat taagactata
     4441 tactttagat attttagata tttatcacca taaacttttt ttatctttac tggaaagggt
     4501 actttatgta gtaaatcttg tacaagtcca aagggacaaa gccaaccaca aacaaatctg
     4561 ccaaaaaaag caccaaatgc cattaaaaaa ccaacaacat aaaatgagaa tttatactct
     4621 ctgcttgata aaactgcttg taatgaacct attggacatg ctccaagtgc acctggacat
     4681 gaataacaat tcaatccagg tacacataat tttttacttt ctcctccata tatagttcct
     4741 tctaaaaatc catttacata tccatttgtt aatgctgtaa atgcaacttg aataataagt
     4801 cttatattat tatttacctt agataataac ttcattcctc ttctcctaac caataccaat
     4861 acactcaaga caaatcataa ttgctttagt taatactgta tttgtttctc cacgatatgt
     4921 acctaatgca ataaatataa tactaattgc aataatagaa atgccttttt tattatcttt
     4981 aataaagtta ctcattggct tttaccattt ttagtctttc atctataatt tttttccact
     5041 cttccttact atttgcacct acaaccttct cacccacaaa gtttcctttc ttgtctacaa
     5101 aataagttgt tggaaactcc ataactcttt taaaatcagt ttttttaagt tcttcagata
     5161 cagtcaattg ttggtaagtt gcatctgact ttttcataat gtttttaact aaatttttct
     5221 cttcatttga aagaccagtt ctcatatcag ttttatcaac ttctacaact aatcccttta
     5281 tagctacatt ggaattatta ctctcatatt cttttgataa tgcttcaagc tctggcattt
     5341 cacccacaca tggtccacac caagtagtcc agacattaat aagagtaagt tctttatttg
     5401 aaaatatctc ttttgttacc ttatttccat caacatcctc cgtatctaaa ttactaaacc
     5461 attcactatt actcttgttt ttttctactg gcttagattc ttttttgcta catccagaaa
     5521 tcacacctaa aacaaaaata catcctaaaa ctaccataaa aaactttttc atatctcaca
     5581 ctcctagaat ttatatagta tataaaatgg ctgcttgaaa taaaaataac ttctcttcta
     5641 tattaaaaca ttatatttaa taaataagaa ctaatttaaa ataagttgta taaaagatat
     5701 atagttaa
//
LOCUS       DAMKPG010000158         5687 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMKPG010000158 DAMKPG010000000
VERSION     DAMKPG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.164"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            69..920
                     /locus_tag="PC621_003498"
     CDS             69..920
                     /locus_tag="PC621_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF4110609.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1053..2666)
                     /gene="ggt"
                     /locus_tag="PC621_003499"
     CDS             complement(1053..2666)
                     /gene="ggt"
                     /locus_tag="PC621_003499"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF4110610.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2891..4828)
                     /locus_tag="PC621_003500"
     CDS             complement(2891..4828)
                     /locus_tag="PC621_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110611.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4815..5582)
                     /locus_tag="PC621_003501"
     CDS             complement(4815..5582)
                     /locus_tag="PC621_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110612.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
BASE COUNT         1929 a          908 c          636 g         2214 t
ORIGIN      
        1 ttagtttata aagttaataa ttaatataaa ttaaaataaa gataatataa acataggagg
       61 tacattcttt gaaaaacatt atttcagcaa aagaacttat atcaaaacta gaaaaaaatg
      121 ataatctagt tgtaatagat tgcagatttg atcttatcaa tagaacttat ggtattgatt
      181 cttataaaaa aggacatatc aaaggcagtt ttatcttaga tatagacaag gatttatcat
      241 ctcctacaaa agaacatggt ggtaaaaatc ctttacaaga ccctttaata ctaaaagaaa
      301 aattagaaaa aatgggtgtt gataatgaca ctacgattgt aacttatgat gatggagatt
      361 taaatggagc ttgcagatta tttttccaat taaaacattt ggggcttaaa aatgtctatg
      421 tactagatgg gggaataaca tctttcgtaa aggaaggtgg agaattggaa gagaaagtga
      481 atataccaag ttgtacaggt aaggaaatta gaccaaatat aaacaatgat ttagttgttc
      541 caatggaata tgttaagtct aaattataca aaaaagatac tgtaataata gattgccgtg
      601 ctaatgagag ataccagggt ttagttgaac ctgcttactc aaaagctggt catataccaa
      661 gtgcaaaaaa ttatttttgt aaggatttaa taaaaagtga ctttgaaaat ggctcactta
      721 aagatataga atttttaaaa aaattcttta aagatttaaa taattatgat gaagtaattt
      781 tatcttgtgg ttcagggata tctgcttgtg ttaacagtct tgctcttaga gaactggata
      841 tacctcacaa gatttatatt ggtagcttta gtgactggat tagttatgat gacaatgaaa
      901 tcaaaactgg tcaagaataa ggctcataat ctatataaaa tcagcatatg atagcttaaa
      961 ttttgtaaag gcgtcagtca ttgtagactt tcgcctttac taattatttc atatactctt
     1021 taaatttatt atatatattt tagttgataa tattaataaa ctgcaatatg accatcacat
     1081 cttgattccg tcccacaaat ataactttcc tgttctgttt taaatattat ttgacctcgt
     1141 cccatgtcga cagtatcatg aattactttt atttcatgac ccttttcaat taatgaatct
     1201 attatatgct ttggcatctc tggttcaact tctatttcct ttccttttat ccactgccat
     1261 cttggagcat ctagcgcctc ttgtggattt aatccaaaat caatcatatt tgttaacact
     1321 tgaatatgtc cttgtggttg cataaaagct cccattacac caaatggacc tattgcttta
     1381 tcttctttac ctaaaaatcc aggtattata gtatgataag gttttttaaa tggttttact
     1441 acattgtggt gtttagggtc taaattaaaa ttatttcctc tactatgtaa agcaatccca
     1501 gttcttggta tcacaattcc tgaaccaaaa tttatgtaat tgctctgtat atatgaaacc
     1561 atatttccat ccttatctgc agtacaaagg tatactgtac caccacaata tggctctcct
     1621 gcagttggat acaaagcctc tttattgtca attaataaac ttcttttttt ggcatactct
     1681 tggcttagta attcctgaat ctttaccttc atatgctcta tatcagttac atatgtttta
     1741 gaatctgcaa aagctaattt taaactctct ataattttat gtatgtcttc tacattttct
     1801 atatttcctt ctaattccaa ttcttttaat atgttaagtg ccattaaaac tgttattcca
     1861 tgaccatttg gtgggatttc aaatatttta tatcccttat attctgtact tataggttct
     1921 acccatgttg gataaaaaca ctctaagtca ctctttctta tagccccatt aaatttttta
     1981 gaatattcat ctattttatc agcaagtctt cctctataaa agctttcagc ctgagtattt
     2041 gctatctctt ctaatgtact tgcctgttct tcacatataa atatatctcc agcctctgga
     2101 gctttaccat ctttagaaaa tgtatcaaac catggtttga actcttctcc aataagttct
     2161 ttttcataga gttcatatga ttttttccat accttagcta cattaggaga tactacgtat
     2221 ccctctcttg catagtttat cgctggagat aagcattcca ttaagctaag tttaccatat
     2281 tttttattta attctgacca agctgcgggt attccaggta cagttactgc tccaaaccca
     2341 tattttggca tttctttcat attattataa ttttttaatt ccatattttc tggagcaaag
     2401 ccactagaat ttaaaccata caatcgtttt tcttcctcta tccaaacaag ggcatatgca
     2461 tctcctccaa tcccattgct tgtaggttct actactgcta atgtggcggc tgttgcaact
     2521 gctgcatcaa cagcatttcc accctttttt agtatttcaa gtccagcttg agatgctaat
     2581 ggtgttgatg ttgcaactgc tccattttta gcatacacga catttcttat tgatgagtat
     2641 ttatgttggt aggcattaaa cttcataaat ttcagctcct cgtcattttt tatttgatat
     2701 tctgcttaga tttattatat aacatcaagt aatgtaactg atacaaaatg atataaaata
     2761 tagacttttt cctaacttta attttatctt taaataaata tcttaaactc aattctagta
     2821 ttttctatct gcacttaaat aagttatttt attcaaaaca tatatctaat tcttttgact
     2881 taaaaataat ttattctttt atcatgttta gagcatattt taaagttaaa aaataatata
     2941 taaaatatcc tataaaataa atgactagag ttatgataat tggtacaagt aatgaccttg
     3001 gtataaactt ttgtgctata gacatagcaa ataaattatg aactgtacct acaactaatg
     3061 gcattaaaaa tataattttt aactgttttg atattatttt ctcaatatct ttattatttg
     3121 ctccaagttt tattaacata atatatcttt ccttatcatc atatatattt gataattgtt
     3181 taaataagat tatactacct gtacataaaa gaaatactac agacaaaaat atgcctataa
     3241 acagcattag accacttaat tttatcaagt tattataact ttcaatactt gatgtaaaat
     3301 taaaaggata agtaaatttc atattttcac ttactatatt tttaaatttt aaagtcaaat
     3361 ccagttcttt tttttcattt tttatatcaa ttaatcttat taatctagaa tttccatgta
     3421 attttaactg attaaatacg tcatctttta ctacaattaa atcaaacgta gcttgtgaat
     3481 ttatttctgg ctccaagatg cttttttgta ccttaaacag atttatatca tactcatttg
     3541 tatctttaat atacaagttt attttttcaa tctcaaagtc tttaaaaaac atttttttgt
     3601 atgaatcaga aacatagtac acagattcct ttgaagttaa ttcttcataa ttgttattct
     3661 gatattgtct taatttttta aaatcacttt ctctaataat atctacagaa gttctatctt
     3721 ctatataact atttccattt ttataataca tttcaatatt tatatctttg tctataagtt
     3781 ctattgtttt atcaaagatt atcttattat cttttttata tttatataat aatttgtcaa
     3841 tttcgttatt tacataagaa tttcctaaat ttatggtata actgtattta taattttcac
     3901 ttatgttcct atctatatcg taataaagtg atatggtaaa ccctagcata gttacactcg
     3961 tagctattaa tattgctatt acagccaata cactactatt gctctttaat ttaaatccaa
     4021 gttcagaaaa tgcaattaaa ttctgtccct tataataaaa actttttctc ttcttaacag
     4081 catcaataat catatacata gttgatgaaa aaaataaaaa agttccaggt attattgtaa
     4141 ctaatataaa aactgaaagc ataatatttt cagcaataaa ataactaatt gacattaaat
     4201 aagaaatagc tattaataaa acaccaagta cacattttaa ctttataaat tttttaaact
     4261 tatacttttc tggatattta ttaaataact ctataattct cttatttctg ataactatag
     4321 tatttcttat tccaataact ataaatatta ttaaaaatac cgctagtgtc ttaactaagg
     4381 cctttatact taagttcata tttactacta tcatttcatt cattaatttt acaagcatca
     4441 tagtcataag ctttgaaaat aacagtccta aaaatatacc tattagtatt gcaactatac
     4501 ccatcaaaaa agtttcatat aaaaatattt ttcctatctg atttttcctc attccaagta
     4561 aactataagt tcctacttct cgcctacgtc tattgaaaaa aaatgagttt gaataatata
     4621 taaataaaaa tacaaatacc actattacaa ttgaacttgt attaatactc atcctagttt
     4681 tttctccaag tgaactcaac gcttcattat actctataga tttaaaacta aaaaatacaa
     4741 atacactaaa tacaattgaa ataaagtaca ttaaataatt aaaaaaacta ttctttacat
     4801 tttgcattgc aattctaaat aaagtcattt ttattaactc ctccaattaa ggaaactgta
     4861 tttactattt tattatagaa ctctctgtta ctttctccct ttacaatttc tgtgaatatt
     4921 attccatcct ttataaagag tattctattg caaaaactcg cacttatagc atcatgagtt
     4981 accatcaata ttgttttatt gctgtctttg tttaatttta atagacaatt caacaaatcc
     5041 ctagcagatt tagaatctaa tgctcctgta ggctcatctg caagtactaa tgatggtttt
     5101 gttgctatag ctctacatac agctcctctt tgcttttgtc caccagaaac ttcgccagga
     5161 tatttatcta atatagattc tatatttaat tcttttgaaa tctcttttat tctattttct
     5221 attactgata ctctttcatt ttttaaagaa agaggcaaca ttatattttc ttttattgta
     5281 agtgtatcca aaagattaga atcttgaaat acaaagccta gattatcacg tctaaaatct
     5341 gccaattcat tttgcttcat tttaatagta ttctttccat ttatataaat atctcctgat
     5401 gaaggtaaat ccattgtaga aataatattt aaaagtgttg atttaccagc accggaagac
     5461 cccataatac caacaaattc tcctttataa accttgaaag ttattccttt tagagcttca
     5521 tgttttgaac cttttcttcc atactccttt tttatatttt caacagataa tatttcttcc
     5581 atagttaata atcacctttc atcacaatta gttatttatc aattctcaca aatatattat
     5641 atatattaaa atggctttta aacatttaat tatattaatt taaggtt
//
LOCUS       DAMKPG010000159         5569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMKPG010000159 DAMKPG010000000
VERSION     DAMKPG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.165"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            150..1331
                     /locus_tag="PC621_003502"
     CDS             150..1331
                     /locus_tag="PC621_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF4110613.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1473..2390)
                     /locus_tag="PC621_003503"
     CDS             complement(1473..2390)
                     /locus_tag="PC621_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110614.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2643..3437
                     /locus_tag="PC621_003504"
     CDS             2643..3437
                     /locus_tag="PC621_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110615.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3438..5498
                     /locus_tag="PC621_003505"
     CDS             3438..5498
                     /locus_tag="PC621_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4110616.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2132 a          630 c          917 g         1890 t
ORIGIN      
        1 taatgttttt gtgtataatt ataacaattt aatttatatt aatgcactta aatttgaaat
       61 agaatgctta tagtggtaaa attaattatt agaatattag aaataataaa taaataatac
      121 attgtactta aaagggggag aaatttaaga tgaacaaaaa atatattaaa gcagggaatt
      181 taattgatgg aacaggaaat agtattatgc aaaataaggg tataattatt gatggagata
      241 ctatagttga aattgaagat ataagagatg atttaaatgg atatgatgtg tacgattact
      301 tagataaaac tgttatgcca ggcattatga attgtcatgt tcacttgaca atggagccag
      361 ttggaaatcc aaagatatat tatgataatg tatcagatgt agagcttgta gtaaatacga
      421 taaaacaagt tgatgcttat ttggattcag gagtaacata tattagaagc ttaggttgtc
      481 ccaaatatgt ggatgtacaa cttaaaaatc ttatagacaa agggtctata gatggtcctg
      541 gtatagttac ttcaggtcca gttatatgta tgacaggagg gcatggatat tattttggta
      601 ttgaaagtga tggagtggat gaatgtagaa aatcagccag aacagtttta aaaaatggag
      661 tagattgtgt aaagataatg gcaactggag gtgtaacaac tgatggagtt gagccagggt
      721 caccacaatt aactttggag gaaatgaagg ctgctgtaga tgaagctata aaagcaggta
      781 aaataactgc cacacatgca caaggaagaa ctggtataaa aaatgcagtt cttgcaggaa
      841 ttacttctat agaacatggt gtttatttag atgatgaaat aattgatttg atgcttgaaa
      901 gaggaactta cttagttcca actgtggctg caccatattt tattctaaat gcaggaaaag
      961 aaagtggaat aactgaagct actcttagaa agtgtgagat tgttgccatt ccacataaag
     1021 aatcattcct aaaagcatat aaaaaaggaa ttaaaattgc agttggttct gatgcaggaa
     1081 cttcttataa tgaacatgga aaaacatatt atgaaatgaa gctaatgtca gattatggta
     1141 tggataatat ggatataata gttgctgcta caaaaacagc atctgaactt ttaagaattg
     1201 ataaaaatta tggaactcta gaaaaaggaa agaaagcaga tgttatagtt gtaaatggaa
     1261 atccagtaga gaatatagac gtacttgcag atgtgttagc agtatttaaa cttggtaaaa
     1321 aagttagata aatagtataa aaataaaaaa gagtgtctaa agaaattttt aaataattat
     1381 agacactctt ttttattagt aataattaaa tcttttatac tttagaaaat agattctatt
     1441 ttatatttag atataactct ttaaaatcat tcttatgcaa tataatctat agaatctaca
     1501 catacaaagt atattcttga atcattagaa tttccaagta ctataacatc atttttctta
     1561 cctagaactt caacaccagt tagaaccaaa ctaccagcta ctacagtaac ttgattattt
     1621 atatttgaca tataaagatt atctaggata cctttattac aacagcaatc atcacaatcg
     1681 cagttacaat tacattcttt gtaatgtttt ttatcaagat agtaacctaa agatgctatg
     1741 aaatcttcat aatctgcagg tgtaatatca aatgcaacag ctttaagatt acacaatgat
     1801 gctaaatcta cgtttgctaa tggagtagat gcagcaagta taaaatcaat tatagcagca
     1861 agtattgcat ctaatatagc cccaaggtct atcgtaggag gaataactgc atcaataagt
     1921 gcaattaatt cagctactcc tggtatagtt cctatagtat taattaatcc ctcaagagtt
     1981 aaagggactg gaataggata agatactcta ccagctatat ctattaaatc acagttacaa
     2041 gcagaaaatc tttcaaaagt accatcaagt ccagataaat tatcttttgg aggagctgaa
     2101 agatctatac ctaccaaatt agcacctact ataaagaaat ctgagataaa agcaaattga
     2161 ttaaagttta caaaaggtct tagagcatca tatcttagaa gttctaaggc ctctctcata
     2221 ctttttttac aacaacattg ttttatttta ggttctttat atttatttgg ataattatag
     2281 tatctatcag ctacttgata attttcatag tatctttcat ttgtatttgg ataatcttcc
     2341 tcatattctt gtgtaaatct aaagtcctct ctacatttat tattttccac taaatacact
     2401 ccttttatat tttaatttaa atgtatagat aattaagctt atctatatag taatattata
     2461 tttataatgt aaaaaaatgt tacaaaagat attataaacg aataaaaaat aataatttgt
     2521 cagataaact tgtaaatttt tatgaataat ccattctaaa acaaaaaact gtgtataaaa
     2581 aaggtttata tgttatattt atataaatat aacatatgaa cgagaaagga gtggtttgat
     2641 aaatgattga ggtagataaa ttgtgtaagt cttttacaag ggttgtaaaa gatgataaga
     2701 ataaaagttc aataagaaag ctaaagaaag taaaaactaa aaaagaagaa ttttttgctg
     2761 taaataatgt ttcatttaaa gttatggaag gtgaaattgt tggaatatta ggtccaaatg
     2821 gtgcaggaaa gactacatta cttagaatgt taggtggcat attaacacca acatcaggaa
     2881 gtataaatat ttctggatat gattattcaa tagatagaaa ttcagcaaaa aaagaaatag
     2941 gttatctctc tggaaataca aaactatatg gaaggctttc tccgagagaa cttctaacaa
     3001 cttttgcaag tctatatgag atgtcaaaag aagatataga agaatcaatt gaaaatattg
     3061 taaaaataat ggatatgagt gaatttatag ataatagaat tgaaaatcta tcaactggtc
     3121 agactcaaag aacatctata gcccgttgtt taatacattc tccaaaagta tatatttttg
     3181 atgagccaac actaggtctg gatgttttaa gtagtgcatc tattatagat tttatgaaga
     3241 atgaaaaact aagaggaaaa actgttcttt actcaactca ttatatggaa gaagcagaaa
     3301 ctttatgtga caagattttt atgattcaca atgggaaaat aattgcatca ggaacaccac
     3361 agagtttaaa agaggaatct ggagtaaata atttgagaga tgtattcata aaacttgcca
     3421 gaaaggagga catttaaatg agaagtaaga ttgtaaaata catatttaaa aaagaaatgt
     3481 tagacatatt gagagataaa aagactttat ttatgatgat tgttgtgcca cttcttttat
     3541 atcctttact tatgttatta atgattcaag taatgaatat gtctgcaagt agtatgaata
     3601 gtaaggacat taatatagct tttaataata aacctaataa ggtgttagta tccatgattg
     3661 aagatgataa tttagaaaaa gatactaaaa atacttcaaa tgataaaggc aaaataagag
     3721 tgttagatgt agataactat aaaaagtcat tagaagaaaa taaaattgat gcttatataa
     3781 agatggaaga aaaaaaatca tctataaatt atcaaatata tataaattct tctcaagaaa
     3841 attcttctaa tgcactaaaa agaattgaaa ctgttttgga taaatataaa agatttacca
     3901 tagaaaaaac tttatctgaa aaaggattag atgtacaagc tactttagaa ccaataacat
     3961 atagcactgt agatgttgca aagacggaag aagtggcagg atacttttta ggtcagatac
     4021 ttccatttat attaataata ggagttttac taggttcaat atatccagca atagatgtta
     4081 tggctggaga aaaggaaaga ggtacattag aaacattatt tacactccca atatcaaact
     4141 tagaactggt tatgggaaaa tatatggcag tttctcttag tgcaatagta acagcaattt
     4201 taaacatttt atctatggga ctgactatgg catttatttt agttagtgga ggtattagta
     4261 gccaatttgg ctttggaaac tttaattatg gagacttagt cttaccaatt ataactactt
     4321 taatttgcat atgtcttttt tcaatggtag tttcagcaat aagtatgtgt gtatgttcat
     4381 tagcaaaaag ttttaaagat gctcaaaact acataacacc tcttatgtta attattatga
     4441 taccatcata tgtttctatg ataccaaata tagtattgga tagagtgaca gctgtaatac
     4501 ctgtagtaaa tatatcttta cttataaaaa gtgtattatc ttttaaaagc gatttaaatt
     4561 taatagcctt agttttagca tcaaatgtag cctttgttat attggcaatc atactactgt
     4621 ctaagatgtt caattctgag gaaatacttt ttggtaatag taagagtttt tctttcttag
     4681 agaaaagaag taatataaaa aaagggtcta taccaactgt aagtgatggt gttattttat
     4741 atgcagtagg attagttcta ttgatttatg ttggttcttt agttcaaatt aaatttggta
     4801 tggcaggtat tataatgaca caattcatga taatttcttt accattatta tttgcatgct
     4861 atattaaagc tgattttaaa gaggtattta gcttgacagt tcctaaatta aagcatgtgt
     4921 ttgggagcat tgttctttgg attggtggat acatacttat tatgattata acacagctaa
     4981 ttttattctt atttcctcaa aatatggaag ttgctgaaag tttaaataag gctcttttta
     5041 tcaaagatag tgttttactg aatttactag caatagcttt attaccagct atatgtgaag
     5101 aactgttttt taggggattt atattttctt catttagtaa atcaaaagat aaaaataagt
     5161 ctatcaaact cgctataata tgtagtgggg tactatttgg tattatgcat atggatttta
     5221 ttagaataat tccaacatca atattaggta ttatatttgc atacagtgtg tataaatctg
     5281 gttcaatatt tgtatcaatg ttattgcatt ttttaaataa tagtgtagca gttgtgttaa
     5341 atcattatac aactggaaat ataaccaact tatataaatt tgttgaagtt gatttttcga
     5401 acttaaatgt acttaaattt gccttaattc ttctaataag tcttatatta attatgttag
     5461 gattaagagt tttagataga aagtctttaa gaaattaagt ataaagttat tatatagatt
     5521 aaaatattaa taagcactaa agtatatgga tatatcttgt atataaaaa
//
LOCUS       DAMKPG010000160         5457 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMKPG010000160 DAMKPG010000000
VERSION     DAMKPG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.166"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(122..2752)
                     /gene="cdtB"
                     /locus_tag="PC621_003506"
     CDS             complement(122..2752)
                     /gene="cdtB"
                     /locus_tag="PC621_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HDF4110617.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(2805..4196)
                     /gene="cdtA"
                     /locus_tag="PC621_003507"
     CDS             complement(2805..4196)
                     /gene="cdtA"
                     /locus_tag="PC621_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HDF4110618.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(4586..5332)
                     /gene="cdtR"
                     /locus_tag="PC621_003508"
     CDS             complement(4586..5332)
                     /gene="cdtR"
                     /locus_tag="PC621_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF4110619.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         1742 a          811 c          647 g         2257 t
ORIGIN      
        1 aaaaagaata tgttagaaga agagaatatt taaataaaaa ataccttata aagttaattt
       61 tttagtactt tataaggtaa atatatattg tattgtgggg acaaatttaa atcatttgat
      121 actaatcaac actaagaact aataactctc tatcgtctgg agtaattgca tatattctta
      181 atttcttgta tgtcataata ttttctccac ctgtaaaata actcctaaga ttaacataat
      241 tagttttagg ctgatttgga tctcctaaat aattacgcat ttctttatct ttagttccaa
      301 tatctttaaa tccagaatat tgtaaagtag cttcaactct atatttttgg atataatcga
      361 gagcttcttt attagtttgt gttggtgcaa aatacatacc atttatataa gtatttcctg
      421 tacttggatt aaaatttaaa tctgcaaaat atattttatg tgcatccatt atttcttggt
      481 cagttggaat tttaacttct ggttcatcaa gtatttcagg agtactattt agctctgtaa
      541 tagataagtt atctaagtat gttgtaccac taccaattaa tgttatctct atattagaag
      601 aatctttttc agtagtttca aattcataac taaattttgt atatccttgt tctggtacca
      661 aataatccgt tttctcttct tttgctttta tctttacaat tattgaatta gatgttaaag
      721 gatcctttga atatccacta aaaacataac gtttataagg ttttagctca gacataggga
      781 ttttaatctt cgtctcacca tttaatttat ttgctgagcc ttgtaaacca tcttgattcg
      841 tagtattgac attactccat gtactagggt aattattata atcatcaaaa ttagtaaagt
      901 aagttggtgt ttttataagt atattcattc ctctttcaag tttaacatta tatatctttt
      961 tatcagacaa agtttttaaa ctatctttaa tcttattggc tgtattatca tcaaatatga
     1021 gttcaacaca actttcatct attggtatat cattaaaata taacaaacca tcttttttag
     1081 tagcgccaaa agctttttca attgcttctc caattgtaag ttcaggtgtt ttatcttctg
     1141 gatcctgtaa atttttagca gtaactcttc tttcgtaaga ttcattctct gtatctaata
     1201 taatagatgc agaaatactg tcaatttgac ttatatagtc tgaccaacta tttccttctg
     1261 ttactatttg tccagaacta ttttttgtac caaaatttcc acttacttgt gttgtttcta
     1321 atttaatttg ctttccagca tctaattttt ttaattgatc ataatttata ggaatcagtc
     1381 tagagctaaa ttgatccatt gtgttaagag ctagaggtga aagccctttt ttgggataag
     1441 tatctccagg agatagatta ttgccaattt gattttcttg tgctttgata gttgataatg
     1501 tatctccatc taacactaaa tttgttgttg gtgtcacttt gtacataggt gcagtacctg
     1561 tgttgtaata tctaacattt gcatttatat atgcagattc tcctttgttt atacttaatc
     1621 cagtattcca tgattctcca ttactatctt gaacagcagt tgaattatct gttgtatggg
     1681 aataatttgt agttacatta gctgtgaatc cattttgata tcctacatta acagacacac
     1741 cagctgtatt agattcagtt ttactgttag tagtagctct ggaaacagtt ttaccttgat
     1801 cagtagaggc atgttcattt gtagatataa ttaatttttc catacctact ccaacaattg
     1861 gatatgctgc aactaacgga tctcttgctt cagtctttat agccttgtca aaagaacctg
     1921 aagctttttc ataatctgta tatggatctc cagcagtatt tgactctaaa taatttgata
     1981 catatttctt atagccttgt tctgcaaaac tatcttccca cttaactgca attaagtcct
     2041 taatagtata tccatttcgt tcatatgaat ctggtatatt atcattatct gtatccaaat
     2101 cttcgtcttc ccaatcagac atcaactttg ggtcaaagaa attgttattt gggataaatg
     2161 gatcatcttt ttctatatta gaataatctc ttaagaataa attttcttct ggtataattt
     2221 tcttcatacc atctaattcc caataaagat taggtgatga taaattatct attgaaccta
     2281 aatttttatc ttgtagctct attctaactt tatattcttt accctttttc atattaactt
     2341 taagtgtatt tgatatagta ctctcagtat ttacttgcat taagacatca tctctatcag
     2401 ttgataatgt atattcacca tccttagaag gaattattct tcctgtccat cgtatagatt
     2461 ttacatctga tttgtcttta tccagaagtt tatctacttt cttttcttca aattttaaat
     2521 taccatcttt tatgggtgcc attaatttta aatctttaaa gtgctcatct gtgaaataat
     2581 atcccattaa tccattgttt gggagtatat cttcatttac aatttctttt ttcttattag
     2641 aatgattact tgtactagtt tgagcatata caggatatac tagtgcttga ctaactatag
     2701 ctgtaagtgt taaaaaactt aatacctttt tattcctcat ttgtattttc attgtttctc
     2761 ctccttaaat ttgaaaaata ttaaattatt tatgtgaaaa aatattaagg tatcaatgtt
     2821 gcatcaacaa ttaattttgt tatagtacca tctttgtaag aatcaatttt attgatttta
     2881 aatttgcttc catgatttaa aagcacttca tattcacctg cataacctgg aatagctgat
     2941 agataagctc caggagaacc tttaggtata gttatacgta gtactatttt tcttttagca
     3001 aatgcactca tattcacact accaatacta gtactaataa agtttggata agacagtgct
     3061 tgtccttccc attttgattt aaaagcatct atattttcta gtttgttaaa atcatattca
     3121 ggggaagtaa gagttaaacc aaattcttga ggaccagatc ttctatatac agttaaatta
     3181 gttggaatag gttcacgttt taatgcattt tcaatgtttg tgattttaga atctaattct
     3241 gggttaggat tatttactgg accatttgat attaaataat tattaattgc agtatatcct
     3301 ccacgcatat aatcatttac atcagcaagt tcattaggtg ttaatttatt actccaatca
     3361 ttataatttg ctttacccca agaatccccc ttacttacat catctttaaa atcaagacta
     3421 cttacaacag atgcttctgc tttaatatag tgcttcccat ctataactat acgaacaatt
     3481 ttatctattt ttatactata tccttgctct attaatgtac ttacattgtt agtatttgta
     3541 tatggtaaca taccagtatt tctaggtaat tttaagtgca taagtaatgg tgtaggtttt
     3601 tcatcacctt ttccaggttc atataaagaa atatctttaa atccatcttg cttaaataat
     3661 ttgttttgta tagtttcttt aaactcatta aatttttcta atgaaatttc gttttgattt
     3721 tctgttctta ttactttatt aaatgcaaat ttttctggag attcaaaata atagacatac
     3781 ataggtttat ctattttatt ttttgatata gcatttcgaa gttctttata ttctttttct
     3841 cgagaatttg cttctatttg ataatcataa aaataatttc ttgtctgaga atatttactt
     3901 atttctacag aatctttttt atatgattct aatgcttctt tttcagatct ttcaagtttt
     3961 tgctctattc tttctgcttc ttttctttcc cattctttag ccttttcttt atctttaaga
     4021 aaatcttctg gtctttctat aggagccttg taagtagtat tgcaaacttt ttcactatag
     4081 ctttgtaagt cttgtgcata aatgttatta ggtaacaaac cacctaaagt tagatataat
     4141 atcaacaata tagatataca attactaatc cttttatgtt tcctaaattt tttcatttat
     4201 tctccctccc aatattagtt taattaactc ttgaacttta atttatttag attatataat
     4261 ctaaataaga attttttata taaatttcat aaaatatgta actatatatc ataaaaatat
     4321 gtatttatat gatatgtcag tacaaaataa caactgaata ttttaaagag actatttcat
     4381 acttttatga tttaatgagc tatcttgttg aatcataatc taggaacaat ggttgcaatg
     4441 tctaatagaa attgtatatg atatagtcat tatgtgtttt ttcacaaaac gattattata
     4501 tagaggattc ttaggaacta ctttttttgt ataaaaaaag agatggttca aattcttatc
     4561 ttgagacatc tcttttttct atttattatg ttttaataat gttctttaaa atatttctat
     4621 atgtattaga tatacgacat atatggccat tactcatttt aatattattt ttctttaggt
     4681 cgagagaaac aatatgatat ttattaacta caaaagattt atgtacacga ataaagcatg
     4741 catctaaatc tgataaagac cttaaacttt tatagaattc aaatattctt gaagtagtca
     4801 ctaattttat tttatgtgga atagcgcttg tttcaaaata tataacttca tcaaatgaaa
     4861 ttttccatag tgaggaatct gagtagatag taaaaaaatt agagcgataa tttttttctt
     4921 taacgtatct tttatgagca attttaatac aagcttttat tttactttca gcaatttcca
     4981 tatcatgttt taatataaag tccataactt ctaacctata atcgaagatc cctaaaatca
     5041 tttctatatg atttgtaaga aatatgattg gactaatata atctgattct ctaatttttt
     5101 gtgctatcca taatccatca cataattcat tacttatctt gtattttgca tctacaacta
     5161 taaaatatat ttttacttta tttttttcag taagttcttt taataacaaa tttgcattat
     5221 aatataatct tataacatcg ttatcaaaac cttcttttat taaaatatta tttatttttt
     5281 cttttagttt tattccaaaa caaacatcat tatcaaaaat taatatatcc actaaatacc
     5341 ctcctataaa aaattcaaaa gtaaaaaaac taaaagtatt ttataaaaaa tacgtaaaat
     5401 aacactagga gataaataaa attaaggata gttagaatta aggtatattt atgcttg
//
LOCUS       DAMKPG010000161         5409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMKPG010000161 DAMKPG010000000
VERSION     DAMKPG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.167"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            207..1043
                     /locus_tag="PC621_003509"
     CDS             207..1043
                     /locus_tag="PC621_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4110620.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1396..2973
                     /locus_tag="PC621_003510"
     CDS             1396..2973
                     /locus_tag="PC621_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF4110621.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2966..4150
                     /locus_tag="PC621_003511"
     CDS             2966..4150
                     /locus_tag="PC621_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4110622.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4168..4767
                     /locus_tag="PC621_003512"
     CDS             4168..4767
                     /locus_tag="PC621_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF4110623.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4791..5261
                     /locus_tag="PC621_003513"
     CDS             4791..5261
                     /locus_tag="PC621_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4110624.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2031 a          566 c         1032 g         1780 t
ORIGIN      
        1 acataagaaa taattaaata tatttcaatg aaaatatgat aaaatatgac aaattaattg
       61 atgacaataa aaaatatttt gttattgtag tattataggg taaattgcaa aatatatacg
      121 aaagtaaaat tttaaatatt gacattaaat taaatatact atactatgta agtgtgatga
      181 gagaattttt ttagagggga tttatagtga atataaaaaa gatatttaat aacaatgtag
      241 tagtaagctc cctagaagat ggcacagaaa taattgtcac tggtgctgga gtaggattta
      301 agaaaaaagt aggcgatttg atagatgaaa atttgatttc taagaaatat tttgttcaag
      361 acgatcaaag ggacaaatac aatcaaatac ttaataagac ctctattgaa tactttaaaa
      421 tttcagaaga aattatagaa aaggctaatg aagttttaaa tactcaagtg aatgattcaa
      481 taatattagc cttaactagt cacatagaat ttgctgtcca aagagaaaaa caaggtataa
      541 aattaccaaa cttaatattg aatgagacaa aacaacttta tagggaagaa tttaaatttg
      601 gattatgggc aattgatgaa attgaaaaaa ggataggaat aaaattgcct gaagatgaag
      661 caggatatat tgctattcac ataatcaatg gtttggaaaa ttctcaaaaa gatgaaggta
      721 ttaatatact agagttttca aaacaagtta ttcagataat tgaagaagta catggattta
      781 aattggatgt aaattcatta aattatacta gacttataac tcacttaaag ttttttgttc
      841 aaaggattct ccgaaaagaa acttacaatg atagtgatat tgaagatatg tataagttag
      901 tgaataaaaa ttacaataaa cctaaagtgt gtactgaaga aattgcagca ttggtattag
      961 acagatttga atataagatt agtaaagaag aagaattata tttaatgatt cacataaata
     1021 aaataacaaa cagtggttgt taggattgta actgataaag caggcaaaac ctaagtcaat
     1081 gagcttaact attagattaa gttttttgat ttaggttttt ttattagata caatcaaaat
     1141 acagacagtg acttttagga ttgtaactgg ttaagcaggc aaaacctaaa tcattgaatt
     1201 taactattag gttaagtttt ttgatttagg tttttttatt agatacaatc aaatacagac
     1261 agtgactttt aggattgtaa ctggttgagc aggcaaaacc taaatcatta aacttaatta
     1321 ttaaattaag ttttttgatt taggtttttc tataaatttg attatgtttt tagataaaaa
     1381 taggagggga aaattatgaa aaaaaataag ttaggtttat ggaacttctt ccaaatgtta
     1441 gggaaaacgt ttatgttccc aatagcacta cttagtgttt ctgggatgat gctaggactt
     1501 ggggcaggtt ttacagaccc taagatgatt gaaatggttc catttctagg gaatgaatat
     1561 gttaacttag ttttaaactt catgttaaca ataggattat ttgcatttaa caacttggga
     1621 gcattatttg caatggcaat tccactagga ttattaaaaa aagaaaaaga gtttggtgct
     1681 ttttcaggat tagtaggatt tatagcaatg catataggta caaattttta tttaacaaga
     1741 gctgatttgt tagtaccagc agaacagatg tcaactaatg gtcaagctat gattatgggt
     1801 atacaaacat ataatacaag tgttttaggt ggtattgtag caggattaat tgtttatgca
     1861 atatatgata aagttagtaa tttaaagata ccagaatcat taggattcta cagtggacca
     1921 agattagttc caattgttag tttaatagtt atgagtatag ttggattatt aatacctatt
     1981 gtttggccac catttttcaa tgcattccaa agtttaggaa gatggatttc ttcagcagga
     2041 ccattaggat atttttcata tgcagtggca gaaagagtta caattccatt tggactaaat
     2101 catcttgtaa catctgtatt ccgttttaca ccaataggtg gaactgctgt tattgatgga
     2161 gaaacgtact ttggtacatt gaacatgttt atggcatatg tagaaaataa tcagattata
     2221 cctcttgatt tagctggtaa aatggaacaa ggtaaattga tgattcaata tggattagct
     2281 ggtgctgcat tggctatcta ccgtacagca aaaccagaaa atagaaaggc acttaaagga
     2341 cttttaatat caggagtgtt gacagttatt ataggtggta taagtgaacc aatagagttt
     2401 ctattcttat tcatatcacc agcattattt gctttccata catttatgaa tggtctagca
     2461 aacatggtac ttccatactt aggcgtgttg atgggattca caggagattt aattgcattt
     2521 ataagttttg gtgtgttaag aggaacagct acaggatggc caatagcagt attagtagca
     2581 gttttatatt ttgcaatata ctattttgta tttaagtggg caattattaa gtttaatata
     2641 aagaccccag gacgtgagga taaagaaata aaagtatctg aaggaaatgg ttcagatttc
     2701 aaaaatttgt ctacatataa aggacaacaa atgatatctg ctttaggtgg tcaatctaat
     2761 atattatcac tagataattg tgtaactaga ttgcgtttaa aactacaaga tgtatcatta
     2821 ataaatgagg atgcaattaa agaggctggt ggtatagcag ttgttaaatt ggatgagcat
     2881 acaatacaag taattatagg aactcaagtg tattctttaa gaaagcagat ggataaagct
     2941 atggaaggtc atttggaggc atgtaatgat taattttgat aaaaaagtag accgattggg
     3001 tacatattgt acccagtggg actatgtaga agatagattt ggtaagaagg atttgcttcc
     3061 ttttacaatt tcagatatgg atttagagtt tccatttgaa ataacagagg tgttgcagga
     3121 aaggttaaat catagagttt tgggatacag tagatggaaa catgatgatt ttagaaattc
     3181 agttacgaat tggtatatta aaagatttaa ttgtagcgtt gatagtaact ggatatatta
     3241 tagtccaagt gtaatgtatt caattagcaa acttattgag atgttaagta atgaaggaga
     3301 tggaatcctt ataaatactc ctgcatacaa tgctttttat gatgtgataa caaataacaa
     3361 tcgtaatatt ataaagtcac atcttgtaaa taggaatgga ttctattcaa ttgattttaa
     3421 tgattttgag aaaaagtgta aaaaatctaa gatatttatt ttatgtaatc ctcacaatcc
     3481 aactggtaga gtatggagtg agttagaact tgttaagatg ataggaattt gtaagaataa
     3541 tgatgtcaag ataatatcag atgatataca catggatatt gtatatgaca atagaatgac
     3601 acctatctta aatgttgcaa aggactattt agattctgta ttcatttgta catcagcgtc
     3661 taaatctttc aatataccag cattgactgg gtcatatgtg attataccaa atgaaggtat
     3721 tagaaataaa tttgaaaata tcactagata ccgtgatttt gtaaattcgc cagcaattct
     3781 agcaatatta gctacaatgg tctgttatga tgactgcgaa tattggttag atgaactatt
     3841 aaaatacctt aaagagaatc ttaaatatac gattgattat ataaaagata atttagaacc
     3901 attgaaattg gtcatgccag aaggctgtta ctttgcttgg atagattttt ccgaacttaa
     3961 aataagtggt gagaaattcc aaaaattatt gatggatatt ggagaagttg ctattatgtc
     4021 ggggtctgta tatggtgaag aaggtgagtt ttatttaagg ttaaatgtgg catgttcaag
     4081 agaaaaactt gaagatggtt taaataggat taaaaaaact atggattata ttgcaaaaag
     4141 aactttatag taggaaggga gatagttatg ggatatttaa aaacaatggt tgatgttatg
     4201 actactgaaa tgaatgccat aagatgttta atagatgaag caggaataga atatgaaagt
     4261 atagttagtg aaattgcaaa ttgtgaaggc aaagtaattt ttatgggtgt aggaaaatct
     4321 gcacatatag gtaagaaatt ggcagctaca tttgctagta ctggtacacc tagctttttt
     4381 gtacatgcaa cagaggcagt gcatggagat ttaggaatga ttgaaagtaa ggatattaca
     4441 attttaatat caaatagtgg aaattctatg gaagtggtaa actgtataaa atatattaaa
     4501 gcaataggtt ctaagacaat tgcatttact tcaaacagaa attcagtact ggcaaaagag
     4561 tgtgattatg ctttaatata tcctgcaaag gatgaagcag accatttaaa tctagcacca
     4621 acaacatcat ctacaattac tttggtttta ggagattcta ttgcatgtgc attatctaag
     4681 agtagtaatt ttggttcttc agatttttat aagtaccatc caggaggaag tttaggtgag
     4741 aagttaaaaa cagctaacaa tggataaaag caaaaaggag agtagttttt atgttcaaaa
     4801 tgtttaaaaa aaaatcaata aaatcaccca tatcaggaaa agtaatagag ttatctaaaa
     4861 tacaagatgg tgtattttct caaaaaatta tgggagaggg tgtagctata gattctacag
     4921 gagacattgt atatgctcca atcaatggaa agatagtagt agtagcagag actaaacatg
     4981 catttgtaat agaattagaa aatgggatgg aacttcttat tcatgttggt ttagatactg
     5041 taaatttaaa aggcgaaggt tttgaggcat tggtgagtat gggagaacaa gtgaaggaag
     5101 gaacaccaat gttgaaaatt aaccgtcctt tgattgaatt aaagggtata tcacttatta
     5161 ctccagttac aataactaat cattcagagt acaatatgaa tacatgtaat gtaggcaata
     5221 gcgttgaagg tgggaaagat actgtaattg agtttaaata aagaattaaa atacaaggtt
     5281 aatatttata aatacctata aacttatgtt ttataagatt gattgtatgg atttattgta
     5341 taaaaaagaa ctctttaatt ataaattaag gagttttttt attttgtaaa tattgtttag
     5401 atttatata
//
LOCUS       DAMKPG010000162         5377 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMKPG010000162 DAMKPG010000000
VERSION     DAMKPG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.168"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(526..1932)
                     /locus_tag="PC621_003514"
     CDS             complement(526..1932)
                     /locus_tag="PC621_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110625.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(1977..2684)
                     /locus_tag="PC621_003515"
     CDS             complement(1977..2684)
                     /locus_tag="PC621_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110626.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2846..3769
                     /locus_tag="PC621_003516"
     CDS             2846..3769
                     /locus_tag="PC621_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110627.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3769..4530
                     /locus_tag="PC621_003517"
     CDS             3769..4530
                     /locus_tag="PC621_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110628.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4542..5279
                     /locus_tag="PC621_003518"
     CDS             4542..5279
                     /locus_tag="PC621_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110629.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1857 a          832 c          694 g         1994 t
ORIGIN      
        1 acacaaaaac tctactttag tagaactcaa tggtgcaaaa tacttcaatt taaaacattt
       61 atcgtacttt aaataaataa ttataataaa atatacttac aatagttaca tagaagctat
      121 tataactaca cagaacaacg tataattaaa acttttttag gatgtcatta tattttttct
      181 taaaattaaa aaacactata ccataattct attttagtag aatttttttg tcaataataa
      241 aattaaatat taactaaaaa ataaaaaaaa tggaattatc taaagaattt aataacctta
      301 caataattcc aaaatattat ttctatgaaa attttgatac catgagtaac tcagccataa
      361 gcgcataaag cacaagttac tctttacttt aataaaactt gacaagaaaa gaatttatcc
      421 ttattctact ctaatagagt tattttgtca ataaagaata aaattatttt caaaatattt
      481 tcattcttca ttcttcattc ttatctgtta ttattttata aaatatcact taattagagg
      541 taattttagt attgttttta gtccctgact atcaatactc ataatatcta atttacctct
      601 atgtgcatta ataattccag ctactatcaa aatacctaga ccatgatttt gacctttggc
      661 atatgcctca cttagtccat tttggctctt taataaacta ttaattcttt cttgactcat
      721 accttttcca tcatcttcaa caattatata gcaaaaatct aaatcagact tagaggtaac
      781 ccttatatta catccttgtt ggttatgagt tatactattt tgtattaaat tactaatagc
      841 cctttttaat aattttttat ctgcatctat aataatatct tcatttagta tatcaagttc
      901 aacataaaag tcattatcca aattattatt tataaactca gaaataagct ctctcaaaaa
      961 cgcagaaact ctaatcttat cacaattcaa tgcttgcata ttatattcaa gtttagatac
     1021 taaatttagg tcatttacta attctctgat tttaacccct tgattgcata taatagacac
     1081 ttgctcttgt tctctatttg aaaggctact gctttcttca agttctccag catatccaat
     1141 tatcattgaa agtggagttc ttatatcatg agatattcct gcaatccagt tagacctagc
     1201 ttcctctttc ttcctaagtt tttcatcttt catttgcaac tctttagata cagtattaat
     1261 agattttgaa atatcactta gtattccttt ttcttgtaaa agtactggtt ctccttttga
     1321 cataagcttt atgccagtaa taattggttt tatagatttc accattttta aacctatgaa
     1381 tagagctatt aaaaaagtaa ctattgcatt aaccagaaac atacctatta ttaacatagg
     1441 aaattgttct atttccttaa tcgtataagt aaggttaaat ttagtgtatt tgtcctttgg
     1501 ataacctgtt actaatattc catttggatg ttcccataca tacacaggat agtcctttaa
     1561 aaagaacctt gaaaactttg ctacatctgt caaagtataa tgacttggaa tttcttttgg
     1621 taaatcaaat ccccatattc tatctccatt gttatcaatt aacattgccc aaactttatt
     1681 tttatgtagt acttccttac tagaatcatc taaaataaac tttccatctt ttaaaatcaa
     1741 attatcttca atcataatca agtttgattt tatttctctg ctatcacttg tatctaaatt
     1801 atcagagtat ttagatacaa atagcatata tgaaaacaga agaaaacctg ctatatttaa
     1861 tacaataaaa agtaatgaaa tcaagatact tgaacctata aatcgcctca atatgtactt
     1921 tacactatcc aaaattaaat cactcctctt gatttctttt attctaatat ctaaaattaa
     1981 ttaatcatca acttatatcc aagacctctt actgtaataa gatgtttagg tgtagatgga
     2041 gttttttcta tcttctctct aacccttcta acatgaacca taagggtatt ttcatatcca
     2101 tacatatcat ctccccaaat ggcttgacat agtgaatcat tagtaacaat tctcccttta
     2161 ttttcataca actttaaaag caacgaatgc tctttagctg ttaaagaaat ctcttgccca
     2221 tctttctcaa cacatgcact atttaaattc acaacaacag aatctcccag tttaaatact
     2281 ggcaactcct cttcttctat aggcacatat acacgattta atatacccac aagccttaaa
     2341 gtaagttcct ttggtaaaaa tggctttact atataatcgt cagcccctaa tcccaaacca
     2401 atcaatctat cttcatcttc acctcttgca gagagaaaaa taactggtat cttagatgtc
     2461 tctctaattt ttctcataat agagaaacca tctccatctg gcaatacaac atctaaaatt
     2521 gcacaatctg gctttacatt tctaaatacc ctaacagctt cttcataact tgatgctaaa
     2581 aatactctac taaatccctc ttttcttagg atttcatcta tcatttttaa taattccttg
     2641 tggtcatcaa ccacaagtat ttttttatct tttattgact ccataatcat caccctcctt
     2701 tagtatacca tatacccatt ttcacaactt tgcctaaata gacttttaag gttattgtaa
     2761 ggtaaacttc agctaactga aatgtaacca agttattata ctgatataag ttaaaagaat
     2821 caataagcac agattaaaaa aggggatgaa acatatggaa atgataatta gaacacaaag
     2881 actttcaaag gaatacaata aaatatttag agtaaaggat atagacctaa gagttccaca
     2941 aggagctgta tatggttttt tgggtcctaa tggagctgga aaatctacca ctcttaaaat
     3001 gcttcttgga cttgctaaac ccacacaagg aaatattaat attttaggta aagagttaaa
     3061 tgaaaaaaat agaatatcaa ttttaaaaga tataggttct ttgatagaat ctccatcata
     3121 ctatggtcat cttacagggc ttgaaaacat gaccataatg caaagactct tagatttacc
     3181 taaaaaaaat gtaaatgaag cattaaaaat tgtaaggtta gaaaatcaaa aaaataaaaa
     3241 agttaatcaa tattctttag gtatgaagca acgtcttggc attgctatgg caataatgaa
     3301 gtttcccaaa ttattaatac ttgatgaacc tacaaatgga ctcgacccag cagggattga
     3361 ggaaattaga gaattaataa aatctctacc agaaaaatac ggcatgactg ttctaatctc
     3421 tagccatctt ttaagtgaaa ttgaccaaat tgccacatca ataggaataa taaatcatgg
     3481 cgaactggtt tttcaagata gcataaaaga acttcataat agaggtcaaa gtcagattgc
     3541 tatcaaagct aagaatgttg tatctgcaca aaagctatta aatactaaag gatatatgtc
     3601 acttatacaa aatgggtact tgactattga aaatttaaaa gactcagatg tggctaaaat
     3661 aaatggtatg cttgtccatt caaatataga tgtatttaga atagaagaac ataaaaaaag
     3721 cttggaagat atattcctag aacttactgg taaggcggtg actttataat gaaatacctt
     3781 ggtctagaat tttataaact taaaagaaag aagattattc tgatgacctt tttatttgta
     3841 gctgttgaaa taatgtggtg tatcgtaaat tcaaataggg ctattacacg taatccagat
     3901 atgttaggtg gatttgaata ttcctatatg cttatgagtt ttgcaagttt aaatggtcta
     3961 ttctttccta tcttaatatc aataattaca tcacgcataa gcgatataga acataaagga
     4021 gatacatgga agcttttaaa atcttctgtg acatctttaa atagtattta tttatccaaa
     4081 tttttatgtt ctgctatttt ggtatctata cctatattga tgcaagtgtt atctattgtg
     4141 ttgtttggac attttagagg ggttattgag tcaatttctg tatctttact tttaaaatac
     4201 acattaggaa caattcttgt aagtattgct atcattgcac tacacctatg gattgcaact
     4261 gtaattccaa atcaaatgat tgcaattaca tttggcatga ttggtagttt tataggtttg
     4321 acatcaggtt tgttttatca aggtattcgt agattgttta tatggtctta ttacctagag
     4381 ttatcacctt taagttatgc ttatgataat gttttaggtt catctgtcta tagaattgat
     4441 atgaattttt ctattacgat tttagttttc ttaattggaa tgctaattta ttatattgga
     4501 agaaatcatt tattaaaaaa agaagtatag gagggattta aatgttaagg aagtcagttt
     4561 tgggagaaat tctaaagtta aaaaattctt ttatatggta tgtattatta gctttgccac
     4621 ttataagtgt gttgattggt tctggtaatt tttatctaaa tcaaggcata ctcaaaaaag
     4681 aatggtacag cttatggact caagttagtc ttttttatgg ggaatttttc tttcctatct
     4741 taatcgcaat cttttgtgca tatatttgta ggcttgaaca tatgaatcat aattggaata
     4801 atgtaatgac actgccagtt aatttaaaaa atatcttttt atctaaactt acagtagttt
     4861 caatacttac agctattaca caagttttct ttgttatctt ttatatagtt gcaggaaata
     4921 tgtttggttt ttctggtagt gtacctaaag aattgactgg ttggattgct aaaggttgga
     4981 ttatttctat ttcaatagct tctatacaat tatacatgtc tataaagatt agaagttttg
     5041 caactcctat tggtattagt ctttgccttt ctctatttgg tatgggatta atagtagcaa
     5101 aacctgctgt tggtatattt ttcccttatt ctatgttagg tagtgctatg ggggttataa
     5161 agcaatctgc tttaaatgtt aatgacacta tagttacaat atgtgttgca tttattacta
     5221 ctatagtatt ttctgaaatt gccaatagaa attttagaaa gaatgatgtt atttcttaat
     5281 ggtaaatgct agatattttt tggtttgaaa tttatattct ttttggtttg aaatttatat
     5341 cccttttatt taaaaatata tatcttttat ttgaatt
//
LOCUS       DAMKPG010000163         5358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMKPG010000163 DAMKPG010000000
VERSION     DAMKPG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.169"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            358..1884
                     /locus_tag="PC621_003519"
     CDS             358..1884
                     /locus_tag="PC621_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF4110630.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1912..2469
                     /locus_tag="PC621_003520"
     CDS             1912..2469
                     /locus_tag="PC621_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110631.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2991..4034
                     /locus_tag="PC621_003521"
     CDS             2991..4034
                     /locus_tag="PC621_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110632.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4145..5287
                     /locus_tag="PC621_003522"
     CDS             4145..5287
                     /locus_tag="PC621_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF4110633.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2266 a          440 c          942 g         1710 t
ORIGIN      
        1 aataaatttt taagtaaata tttttataga gagattgtta tattacatat cagaaaaata
       61 ttaaacgaac taaatcaata aaaaagttgg tggctaacaa tttataattg ttagcacaat
      121 ataatcttat gtattgaaat ctagaatatc ctcattttta cttagcataa atactattga
      181 ttaagaaaat aatatagaaa ttataaatat atataagttt agtctggtgt ataaaacaag
      241 aaaactaagt gatttttcat ttagcttttt attaagctta ttttttatac tatttataat
      301 aaataaactc catgatggga aaactattac tatttaatat gtaaaggagt attttaaatg
      361 aatgaatcat attggttttt aaactcaagt ccaaaagaat ataacaaatt aggtgaaaat
      421 ataaaaacag attgtcttat agtaggtggt ggaataactg gattgacaac tgcatattta
      481 ttagcaaaag aaggtaaaaa agtagtgctt gttgaggctg ataaaatagg atatggaact
      541 tctggaagga atactggtaa ggtaacttgt cagcatgata tattttactc taagatagag
      601 aaaaaatatg gattagataa agcaaaatca tattataatg caaataatga agctttaaat
      661 ttagttgaac aaataataga agaaaacaat ataaaatgtg attttaaaag ggaaacttct
      721 tttgtattta cagaaaaaga ggatactata aagaatatta aagatgaata tcgtacatgt
      781 aaaaaaatag gaataaattg tgagtatcat gaaactatag aaaatattcc attagatata
      841 aagggggcta ttagttttac taatcaaggt caatttaacc caaagaagta tattgatgga
      901 ttggcaaaag cagcagtaaa tttagggcta aaaatatatg aaaatacacc agtagtagat
      961 cttgaaaaag gaaaaatatg tagggttaaa acaagagaag ataatataat agaagctgag
     1021 aatgttataa tttcatctca tagtccatgg tatgatggat tgaattttta ttttgcaaaa
     1081 gaatatgcag agcgtgctta tttaatggtt gcagttttgg aaaataaatt agcagatggt
     1141 atgtttataa gtatagatga cccatcaata acttttagac aatataataa tggaagtgaa
     1201 aatttactga tttttggagg aggagaccat aaagttggcc agggaggaac tgagaaagaa
     1261 atatttgatg atttagagca ttatggtaaa gaagtcttta aggtaaaaga ttttaagggg
     1321 aaatggtctg ctcaagataa tatgagtttt gacaatgttc cttatattgg ttatatcaac
     1381 aaaagagaag acaatattta tgtagctaca ggattctcta aatggggaat tactaatgga
     1441 actgctgctg gaataataat aaaagatttg ataataaata ataattctga ttataaagat
     1501 acttttaatc catccagatt aggtagctac ttttcaaaag attttataaa ggaaaatgca
     1561 aacgtagcaa taaattatgt aagtggaaaa ttaaaaatag gtagtggaga tatgcctaaa
     1621 aataatggag aaggaaagat tgttaatatt gatggaaaaa gatatggtgt atataaggat
     1681 gataatggag atttttatat tgtagacacg acatgtactc atttaggatg tgaacttaat
     1741 tttaacagtg aagaaaaaac atgggactgt ccatgtcatg gttctagatt tgactataag
     1801 ggaaatatac ttgaaggacc agctcttaag ccattaaaac tttatggaca tggtgataat
     1861 gatgttaatc ctaagttatt ataaaattat ttttaggaag gtaaagtgta tatgaataaa
     1921 cttttaaagc caataactat actattgtat atagtttata ttttatctgc cgtatttttt
     1981 gtagcaaaca aagaatatgg aagtctaggt ctagtagcaa ttagtttagt tggtactttc
     2041 attttgagtt ttataaataa gcaaactgaa agattattga gtaaagaatt atatataatg
     2101 cttgctttat ttataatgtt ttcatcattg ttaggtactt gttttaattt ttatgatata
     2161 aattattatg atgatttttt gcatttatgg tcaggtataa tatcttgtat tgtggctttt
     2221 tctataatta aatactttta tacacaaaga gagataagta aaatgagtaa agtgttttta
     2281 gttatatttt tatttatgtt tagtatggga gtagcgagtt tatgggaaat aggtgaattt
     2341 ttaatggata cttttgttgg gactactaca caggccgggg ggttagaaga tactatgatt
     2401 gatatggtag atgctttaat aggcacaatt ataactattc cttttatagt aaaaaaatct
     2461 aatatatagt tttaaatttt ttatattaat tataatggat ttaattaatc atgttgagtt
     2521 tcaaggtcta tatgattaat taaatccatt tttgttattt ataattatta ttgaatgtat
     2581 ttttacatat aagatataag gtgatagata gaaattgttg gattatgtaa ataattaata
     2641 tataatacca ttatgggcaa taaatacaaa tcaaatgata atatagtcta tttatgtaaa
     2701 tgttatattg tatcctaagt ataatagtaa gttttttgat agtggtgtag aaagtaaaaa
     2761 tctactagcc taaattgatg tatggattag tttaggaaca gtaattgcat atcttaagtt
     2821 tgatgtcatg ttgggctact agaaataggt catctttttc acttgaatat cttttcttta
     2881 aacatgagga gtatcatatc aaaaacatat gaaaattaag tgatttataa gaacagtaat
     2941 attttatatg atagtataga atagtaataa agtattagga ggaacattga gtgttaaaaa
     3001 agtggtttgg tattgtgaaa aaaagacaaa aaagtgagtc agtaaaagaa gaaggtgaag
     3061 taatattaaa aaacgaaaaa atattatctg aagaaaagtt gatagatgaa gaaggagttg
     3121 tagttaatat tgataatgaa atattaacta aagtagaagt agtaaatgat gataatgaaa
     3181 tagaagaaaa aataatagaa gaagattggt taattagtga aaataccata aaattagatg
     3241 ataaagaagc aattattaat gataaaaaca tagaattatg taaaaaagaa gttcaagttg
     3301 aaagtgaaaa aataggttta aataagtttg aaggacttga ccaaaatgaa aattataatc
     3361 tagaaaaaaa tgttattgaa gaaaaaaaag taagtgaatg tttgacagaa gaagatttag
     3421 agtacataaa agaaattaaa ataaaaagag gaaaaagtat aaaagctata aacttgtata
     3481 ctaaagaaga gtgggttttt gacactcata tacagtgtag taaaaaactc aaagttccat
     3541 taggatacat aagagaaaat ttaaaatatg gatatatgga ttactttgga gatgcaataa
     3601 attatttaag tgaagtatta aatatagatg aatactgcaa aggtgaatgg agctatctcg
     3661 ataatagcaa atctccatct gagatattta atattctaaa caataaaata tttagtataa
     3721 ggctttctaa tgaaaaaaga aatgaaattt tgacaaatga taaaattgaa gcattaaaaa
     3781 tgaattatag atttgaatgt attgatgaag aatatgatga atattttaaa aaatataagt
     3841 ctataatcaa aaggggtgga aagaaaaaag ttgaattagt aaataaaaaa ggtgacattt
     3901 tagaaatatt taagtcttta gaagaatgtg ccatttattt gcaaaaggaa aagaatgaag
     3961 ttatacaaat gttaaaatat ggagatacaa aagtaggaag aaactttata aggtatagtt
     4021 tgagaagtat ttaatttcat tttaaattca taagagtttc atttctgatt cacacattta
     4081 atattatttt aatgtaaaat aataaaggtg aagtattgca caattaaaat taaggagggt
     4141 attaatgtct gaagaaaaaa atccaaaaga aaatacatta gatgaaccta aaaattcaga
     4201 taatgtaaat tctaaaaaag atgatgtgtc agatgaaaaa aatactgata acaaagttaa
     4261 aaaaagggaa ataaagctaa acaacaaaac acttaaagct attgtagcaa ttgttgcagt
     4321 aggagctata tgtttaggtg gaggctatgt ttttggtaaa gaagctggaa gaaaattgcc
     4381 tgctacttca aaacactata atagcagtga ggtagtagct actattgggg ataaaaaaat
     4441 aacaggtgaa atgttaagaa agagaatgga gccattattt tatttaaatg caaaaactac
     4501 tatgactgat gacgaaatac gttcatatga atcaaatatg attgaaacta tgattacatc
     4561 tgatatatta tcctctgaag cagcaaaaga gaaaataagt ataactaatg accaactaga
     4621 tgctcagtat aataatttaa tgagtagtat agaatcagca atgggaatga ctaaagaaga
     4681 gtttttaaaa cagtttgatt tgactgaaaa atatataaaa gatgatatga aaaaagagct
     4741 agtagcatca aaatatttaa atgaaaaatc aaaagttact gataaagaag cagagaacta
     4801 ttacaataaa aacaagagca attatttaca agttagagct tcacatattt taataaaaac
     4861 tgttgatgat aaaggtaagc aagttagtag tagcaaaaaa gctgaactta agaaagaagc
     4921 tgaagaaata cttaaaaaag cccaagctgg ggaagatttt gctactctag ctaaaaaata
     4981 ttctgaagat agtagtgcag aatcaggtgg agatttaggt ttctttggta aaggtcagat
     5041 ggtggaatct tttgaaaaag cagcctttgc attgaagaag ggagaggtat ctaacaaact
     5101 tgtagaaagt gattatggat atcatataat taagaaaact gatgaaaaat atcaaccatt
     5161 tgaagaggta aaatcagatt tagtaagcag tttaacatca gaaaaacaaa acctacttat
     5221 acaaaattta aaagaaaaat ataatgttaa aattacagat aaatatcaaa tgaaagctaa
     5281 agaatagata aagtcaattt attaaaaaaa agaaaaatta actgtatgga taaaagctat
     5341 ttttactctc aatataga
//
LOCUS       DAMKPG010000164         5347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMKPG010000164 DAMKPG010000000
VERSION     DAMKPG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.170"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(204..1388)
                     /locus_tag="PC621_003523"
     CDS             complement(204..1388)
                     /locus_tag="PC621_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF4110634.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="PC621_003524"
     CDS             1761..4112
                     /locus_tag="PC621_003524"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF4110635.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="PC621_003525"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="PC621_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF4110636.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2089 a          593 c          850 g         1815 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gtttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaatc
     4861 gattcttatt ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatgtta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaag
//
LOCUS       DAMKPG010000165         5180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMKPG010000165 DAMKPG010000000
VERSION     DAMKPG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.171"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            43..1815
                     /locus_tag="PC621_003526"
     CDS             43..1815
                     /locus_tag="PC621_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110637.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1837..3258
                     /gene="ccpM"
                     /locus_tag="PC621_003527"
     CDS             1837..3258
                     /gene="ccpM"
                     /locus_tag="PC621_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF4110638.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3287..4357
                     /locus_tag="PC621_003528"
     CDS             3287..4357
                     /locus_tag="PC621_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF4110639.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4373..4624
                     /locus_tag="PC621_003529"
     CDS             4373..4624
                     /locus_tag="PC621_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF4110640.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4675..4878
                     /locus_tag="PC621_003530"
     CDS             4675..4878
                     /locus_tag="PC621_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110641.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2108 a          451 c          831 g         1790 t
ORIGIN      
        1 taaaatattt tatttcaatt ttattaagta tggaggtgta ttgtggaaaa tggtaaattt
       61 caatttttta aaggtgtaag aaattgtttt aatatgattt atctacatga taaaagatat
      121 ttatttatct ctatgtttat ggcaattttt caggggattg ttccatctat ttctttgtta
      181 gtaagtcaaa atatcattaa taatatgcaa acattttcga aaagtgtaaa agaaatatta
      241 gttttgttaa ttatgtattt aatcataaat ataattcctt caatttttca aagtttttat
      301 ggatattata gaataaagtt taataaaaat tttgaaaaac atattgaaac aatgatgtta
      361 gagaaatctt taactttgag tttaaaagat tttgaggatg ataatactta tgacatgata
      421 aatagagctc aatctcaaaa aggatttaat attactatac tatctgaaaa ccttatttca
      481 ataataagag aatcagtatc tacactttct cttattgttg tattatcaca atttcaactt
      541 tggctaataa tagttgttat tgttattcct gtattgaaat cattatattc cattaaatta
      601 ggaaaggaac aatatgatgt tagtgtaaac agaacttgga aagaaagaaa ggcctggtac
      661 atagattatt tgttgtcaaa aggttatgct tacaaagaga ttatggtatt tagaattggt
      721 tctaaactta ttaatcatta taaaaaatta aaagaggata ttatatctca agatatgaat
      781 atagctaaaa agaattttat tgctacgcta ttatgtacag ttttagattc aattgtatct
      841 gcaggaatta taggatttat aataattcaa ggtattttaa agaagatttt aataggagat
      901 gcgactacat atatacaagc agttcaatct ataaaggata atgttgaatc aatattttcg
      961 ttatttagtt cagtaataca agatcttttt tatacagatt tactgtttaa atttctagat
     1021 tataactgta aagaagactc aattgaacat ataaaaatat ttgaaaaaat aggggatata
     1081 aataaaatag agttaataaa tatttcatat aaatacaatc attcatcaga gtattcttta
     1141 aaaaatatta atttaacttt aaataaaaat gatcctattg ctatactagg aaaaaatggt
     1201 tcaggtaaaa cgacactttt aaaaatatta ttaggttttt atgatgatta tgaaggtgac
     1261 atcttgataa atggaaaaaa tttaaaggat attgaaaaag atgaatacat tagaaaagta
     1321 agttgtatgt ttcaagatta tattaaatat gaggctacat taaaagaaaa tgtcgccatg
     1381 ggagatgtat ataagagtaa cttggaagaa aaagttaaaa gtaaacttaa agaaactaaa
     1441 ttgaaaccaa gtatttatga ggaagatgga ttagaaacta ttttaggaac ctggtttggt
     1501 aagaagcaac tttctatggg tgaatggcaa aaagtagcaa tatctagaac tcttatgaaa
     1561 gatgctgata tgtacatttt tgatgaacca gattcatctt tagattctga agcgggaaat
     1621 gaactccttt ctttatataa aaaaacattt gaaaataaga ttggaatatt tataacgcac
     1681 catatccaac atgcaaaaaa gataactaac aatattgtag ttcttgaaaa tggaaagttg
     1741 ttagaaagtg gaagacatga tgaacttatg gacaaaaagg gtgtttattt taatttattt
     1801 tgtaactgca aataaatatt aaagggaggt taattaatga atcaaaaacc atttatacac
     1861 acatttaaag ctggaaaatc atattttatt tatgatgtta atacggataa gattttaaag
     1921 gtaaatgctg ctgtttataa ttatctaaat aaaattgagc ataatttaga aaaagaaagt
     1981 gactggaaca tagaaataga agacgaaatt aataccttga ttaattatgg atttctaaaa
     2041 aataaaaggg taaataaaac aatgcaccct gaaactaagt atataaaata tcatatagaa
     2101 aataatctat cctctattat tttacaagta acccaaaatt gtaatttacg ttgtgactat
     2161 tgcatttatt ctggttcata cagcaacaga gttcatacaa ataaaagaat gaatatagat
     2221 gtagcaaaaa gaagtattga ctatttgatt aagaattcta gtcaattaaa tgaagttagt
     2281 gttggctttt atggaggaga acccttactt gagtttgatt tgattaagca gatagtagaa
     2341 tatgtaaaag tagttggaga aggaaaggaa atatacttta atctaactac aaatgcaact
     2401 ttatttaatg aggaaataat agaattcttt gtaaaaaata atataacaac tatgattagt
     2461 ttagatggag gaaaagaaat tcatgataaa agtagaaaat ttgcaaatac aggagaagga
     2521 tctcatgatg ttgtattaaa aaatgtacgt tacataaaaa ataaatatcc agaatattat
     2581 aaaaagaata ttacatttaa tacagtgata aattcagaaa atgattacat ttctatagat
     2641 gattttatat ctaaaaatga gactttaaaa gattcaaact ttttagcttc cactcttaat
     2701 aataactatt ctgataaaga aatagtttca tcagatgaat atatattagg taagagttat
     2761 gatagattct tgactttact caacaaatta aaatttataa aagatgagag tatgtcacct
     2821 ttatcaagaa tagaaagagc cttattagat gattttagat atgagaaaaa tattgaaggt
     2881 agattagagt taccagatgt gtggcatcat ggtggaccat gtataccagg ggcttttaga
     2941 atatttgtaa ctacagatgg agaatttttt ccatgtgaga gagtaagtga aagttctaaa
     3001 atgggtatga ttggaaattt agatagtgga gtagatatag aaaaggctga aaaactttta
     3061 aatataggaa aattatatga agagcaatgt tctgattgtt gggcctatag ttattgtgat
     3121 atatgtatag ctaagattga tgatactaaa tgtaatgaaa atgaaataaa accacatgat
     3181 tgtgacagaa ttagacattc tacagaaaat aatatgaaag attactgcgt tttgaaatct
     3241 ctaggatatc aatattaaat tttctaatga aaggaaagat gggattatgg aaaaagtact
     3301 tatatatcca tttaattata atttttttac tattttaaat cattgcaata aaatttcaga
     3361 tatagaaatt aattcactgg ttagtccttc tggctggggg ttatgcaata aggagtataa
     3421 tgttaataat attattacta aagttacaaa tgattttcaa agtgaactta aaaagaattc
     3481 tttggtatgg tttgttgatt caaaagaaaa tctagactta gaaaaattta taattcctaa
     3541 attaaaagta gctctaaaag aaggtaaaaa gatattatat acacgtgatg atcttaataa
     3601 aatatctagg tatatatctc attatgaatt caaaaagatt aatgaatgga atttagaaaa
     3661 atatgaaata agagatatag agggtatatc acagataaac acaccaataa tatttatttg
     3721 tggtctttat ggagggttag atacttttga tgtaaatata aggataggag aagaactaag
     3781 aaaaaggggg ttttcaatat tacaatttgg tcctaaaaaa gagagctatt tatttaaaat
     3841 gattcctata ccagagttta tgtttgatga ttcgataagt agtaagacaa agattattaa
     3901 ttttaaccat tttataaaag ataaagaggt agaggcaaag ccagatttaa taattataga
     3961 agttccagga gaattattcc ctttctctag aaaaatggtt ggtaattttg gaattagagc
     4021 ttttgaaatt tctaatgcag taataaatga ttgtggtata ttatgtattc catcaagtat
     4081 atatgataga gaatattgtg aagaacttaa atattttatt aaaggaagac atggtataaa
     4141 tatagattat tttagtatag agaaaaaagt tatagataca tatgagacag aggtgaagca
     4201 aaaattttca tatatgtcat tagatgaaaa cttttttgtt gaagatatag aagattatga
     4261 aaaaacattt tttatacatg ataataaaca attactagaa ttagtggaca atatagtgaa
     4321 tcaattaaaa gagtatgcta aagtaatatc tatttagaat tggaggttaa ttatgaatta
     4381 cactgaaaag gaattactac tagagaatat attttttgat gtgactggaa ttaattttaa
     4441 taaaaataac aatttaaaaa aagaattatt tttttcaccg tctttacata tacagccaag
     4501 agaattactt ttagtatttt gcgaggtaat agaaaacttt aatataaaaa ttccagagga
     4561 aaaacttttg aataggcaat ttacgagttt tgataatgtt ctaaaaataa tcaatatggt
     4621 ataggttaat aaaggtataa agtaagtgat cttatagaaa aggggaggat atgaatgagt
     4681 tttggaaaaa gaatagaaat gaataatata ggtgttgaga caagaggtgt ttgtactatt
     4741 atgtgtcctt atacttgtaa gatgatggca tcttcatctt gtaactgcaa gaataatgat
     4801 tataatgcaa gaggaggatc atataataca tattataata aattgataca tggtcaaaga
     4861 actcctgatg gaatgtaaat atttcagtaa taagtatact attaaattga tttagcatat
     4921 ttgattaaat ttatctttct tactttattt ccttttatta taaaaatatg cagttgatta
     4981 taattaagag tgtgatttta taataactgc ataaatagta tctgttatat aaatcacctc
     5041 ttcaattatt atagtggtaa atatagtgat taggggtgat ttttacaatt taaaagtata
     5101 aattaattaa aagttattat aataaattat acttatttaa atagtaagaa ttaaaagtaa
     5161 agtttttaaa tatatttgta
//
LOCUS       DAMKPG010000166         5133 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMKPG010000166 DAMKPG010000000
VERSION     DAMKPG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.172"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            156..1049
                     /locus_tag="PC621_003531"
     CDS             156..1049
                     /locus_tag="PC621_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF4110642.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            1097..3397
                     /locus_tag="PC621_003532"
     CDS             1097..3397
                     /locus_tag="PC621_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110643.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            3631..4122
                     /locus_tag="PC621_003533"
     CDS             3631..4122
                     /locus_tag="PC621_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF4110644.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            4150..5097
                     /locus_tag="PC621_003534"
     CDS             4150..5097
                     /locus_tag="PC621_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110645.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         2204 a          468 c          837 g         1624 t
ORIGIN      
        1 ttgtgtgata gaaaactata tattataaca atagtagtgc ctttaattaa atatatttca
       61 taaatcttca taaaatcttc aaaatttcgt aaagaatatg tcacaaaagt aaggtatatt
      121 atattcagca gatagtataa gggaggacaa tatttatgaa gaataaaaaa ttcattgtta
      181 taatactgat aatatcaatt ttaataggtg tattagtgaa ggaatattca tctagagaga
      241 taaaaaagga tgatataaat gtcagtaagt atataaaata tgctgattta gcaagtaaaa
      301 ataatgcaca agttaactgg aagtatgtag cttccattgt tgctgtgtta aataaaaata
      361 acttaaaaaa cgttaaggat tcacagatac aagaagtatc agatttattt gttaagaatt
      421 tttctaaaaa taataaaata aataagttaa gtgatatttt ggatgagtta gaatttagta
      481 atagacaaaa aagattggtt gataattata tagacaatct taaggattat ggtataaaac
      541 cagaaagatt gaagtctgat acaaaatata tgaagtttat tgctgaaatt aaaacagaag
      601 caatacaaaa ttataaggat tataaaatac ttccatctat aactattgca caagctataa
      661 tagaatctag ctggggcaag tcaactttgg caaaacaata taacaatcta tttggtataa
      721 aggctgacgc ttattggaaa ggtaagtcag tgactttaga aacgaaagaa catttagata
      781 caattataga tgataaattt agaatatatg atgataaaaa tgaatctata aaagaccatg
      841 caaaattttt agccacaaat aaaaggtata aaaacaatgg tgtatttgat gcaaaaacgt
      901 atatatatca agctaaagca ttagaaaagg caggttatag tactgcgaag gatgaaaatg
      961 gcaatagtat atatgcagct cgtctaatag aattaattca acagtataat cttcaattaa
     1021 tagacagcga aattcaaagt gaagtgtaac ttttatgtaa cataggagtg atatatttat
     1081 cgaggaggtg gtttttatga aaaaaatatt ctcgatattt ataagttgta tggttgttgt
     1141 aactactatt actggatgta gtagtggtga ggtgataaaa aatacatcac ctatcaatag
     1201 tgttaaaaat ttggatttta gttttgaggt gaaacctgaa atatttgagg tatcagttga
     1261 atctggtggt tcaaaagaaa agatatctga accctcaaaa gaaaggaaag tatcaaattt
     1321 taaaaagaat aagacagagg catcgtggac gtatcctgat gaaaatatag acataaatat
     1381 aaaaaaagag gacagctatt tagatgtaaa tattaaatct aatttagaaa aagaaactag
     1441 ttttaattgg cccaaagttg aaggggaaag ttatatacta ccactttatg agggtaagtt
     1501 tataccaagt aatgataagt attggaagga gtatctaaat gagtcaactt ataatgttat
     1561 agaatctttt tcaatgcaat tctttgcagt aaacaaggca aagtattctg tgatttatat
     1621 tatcgaaaat gaatttaaca atgtaattga atttagttct aaagacaaga ttaattttac
     1681 atttaaacat gaatatccaa gtatcaataa aaataaggag tatgggttta aaatatattt
     1741 gactaataag aatattacag atgtagcaaa aacttataaa aattatatag ttgataaagg
     1801 agaatttaaa acattaaaag aaaaatctaa ggaaaataaa aatattgaaa aactttatgg
     1861 agcaccacat atttattttg gagataagtc tatcatcaca ataaatgata ttgaatggaa
     1921 taaattaaaa gaaaatttat cagatgattt gactaattgg atgaaagagc ttttaagtat
     1981 gaagtctggc aatagtgaat ttataaaagt aattaatcaa ataaaaactc aagattatat
     2041 agataaatat caaaaaaatc aaatagtaca agcatttaat gaagttatgc agatgaatac
     2101 tttttataat gaaaaagttt ttgaaagcaa agatagagaa ataaaaaaag tattagataa
     2161 aggtatatct aaattaagta gggtagaact tatagagttt aataagaaat tattgaaatc
     2221 aaaattggaa gagagcgtga atgatgttgc tacatgggca aaatataata caactgatgt
     2281 aattaatgat attaataagt ctggtattaa aaatgcgtgg ataggatttg atgatatgga
     2341 ggaaggttat atatccttag agatggttaa aagagctaat gagttaggtt atctcgtagg
     2401 gccatatgat tcataccact ctatacacaa accaggggaa gaaaagtgga gtacagcatc
     2461 atttaaagat aaatctctat atgaaaatgc taccatagaa aataaaaaag gtgaaaaaga
     2521 aactggattt caaggagaag gaagaaaact aaatcctaca ttgtctttac caagtgtaaa
     2581 agagagagta caaagtatat tggatgaagg atatgaattt aattcttggt ttatagattg
     2641 tgatgctaca ggagaaatct atgatgatta ttctcaaaat catataacaa cacaaacaca
     2701 agatttaaat gctagacttg aaagaatgtc atatataaga gaccatgcaa atatggtcat
     2761 aggttcagaa ggtggaaatg atttttcaag tagtactata gcatttgcac atggtattga
     2821 gacaccagca tttgcttggg tagataaaga tatgagtaag aataaaggca gtgaatatta
     2881 tgttggtaaa tactattctc caacaggagg agtaccagaa atattttcaa agcagatacc
     2941 tgtaaaagaa aggtacaaaa aaatatttat agacccaatt tactctctac ctttatttag
     3001 attagtttat aatgattctg tgatatcttc ccatcagtgg ttatggggaa ctttgaagat
     3061 taaggatgaa gttggaagta gaatgatgaa agaagtatta tataatacac cacctatgta
     3121 ttatatagat agaactgaat gggaaaaaca taaagatgaa ataatctcaa acaataaagt
     3181 atggtcagcg tttaataaga aagctattaa aaaagaaatg acagacttta aatttatttc
     3241 tgaggataga ctagttcaaa tgactgaata tggagaagat ttaaaggtat tagttaattt
     3301 ttctcaaaaa gacattaagt acaatgatga agtcataaaa gcaaattctt tggtaatata
     3361 tgatgctgat agtaaagtca tatataaacc ggaataatag atttaatgaa aataaagatt
     3421 gtttaaattt caagtaaaaa ataatataat attcatttag tttgtattaa agtagtgact
     3481 atattaatct ggataattag tatagtcact attatttata ttctttccta aaagtaagta
     3541 gtaactgaat cgtgaataat caaaaatggt aatttgaaat agtttttatg aatagtatta
     3601 atatatagat atagggagat tgatgtgatt atgtatgaaa ataataaggt aatatataat
     3661 tttgaaagat gtgaaaagct agaaaatgct ctttatttat ctcaatttaa tacatataaa
     3721 aactttaagt taacttctaa aaaaactgta ataaactcat tagatacatc tacttataat
     3781 atgaatattc atagcataag tctcgttgat actatggaat gtatatcgtc agaagatgaa
     3841 caaatgacag gaaagaagtt gattttagtt ggcgatatag atactgatgt aacattagat
     3901 tatgcaggca ataaaagaaa tagaaattct agtaaaaaaa atacatttaa attaaaaata
     3961 ccctttagta catttataca aatgcctaga aagatagaaa ataaagataa aattaattta
     4021 aagtatctta tacaagatat tacttcatct attttagatg ataatttatt tattagtgtt
     4081 actgcaatta taagttatga aaatagttcc aagatagagt aatctttaaa ttatagaaag
     4141 gtggatgcaa tgagaataac gaaaactact tttaatgatg tattttcaaa tgatgcaaat
     4201 ggaagtatta taaaagtaga aaaatatgtt gataaaaaat gtgcatctgt atttgagatt
     4261 ttgacctaca ctattattgt tacaaataca agtaggtaca aaactggaaa tatatttttc
     4321 aaagattata tttctaaata tatagagttt ataaataata ctgtaaaagt taatggcatt
     4381 ataaaacgtg gattagaccc acagcaaggt ttttacatag ggagaataga tgcaagttgt
     4441 aaaaaaataa tatcttttaa gagtgtagtt ttaccaaatt ctgcacatag gattataaag
     4501 aatagtgcta atatatacta ttattataag tgtaatctag ataaattccc aacaagaata
     4561 tcaatagaaa gtaatagagt ttatactaat gtaaataaaa ttgtattcaa acaattgaat
     4621 ataagtaata ctttaaaagc tcctaaaaat ctaagagata tattaaaagt aaatgtaaat
     4681 agtaaaatat ttgatgttaa acctatgaaa aatcttataa acaaaggtag taatttgaaa
     4741 ttatgtaact cgatagtttt tgggtgtata gaagttgaaa tagactatag ttgtaaaaat
     4801 aggagtaatt ttaaaacagt aaataagttt aatgatatta aaaaagataa gtattataaa
     4861 aatgatatac aaaataacaa tgaaaatata aacgattgta aaaagcaaat aagttttggt
     4921 gataataagg taaaaaaaat aacaaaaact tttggatttt cttgtttcat ttgttctcca
     4981 gttggaatta tttatgaaga tatgaaaaat ataaatataa aaattgaaca tacatcaata
     5041 aatgaattaa atccagaaga attatttgta aatacatcac ttttattgta ttattaagta
     5101 ttttaaagct aaaacttaaa cttattaaaa tag
//
LOCUS       DAMKPG010000167         4997 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMKPG010000167 DAMKPG010000000
VERSION     DAMKPG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.173"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            136..276
                     /locus_tag="PC621_003535"
     CDS             136..276
                     /locus_tag="PC621_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110646.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(273..644)
                     /locus_tag="PC621_003536"
     CDS             complement(273..644)
                     /locus_tag="PC621_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110647.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1193..1450
                     /locus_tag="PC621_003537"
     CDS             1193..1450
                     /locus_tag="PC621_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110648.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1560..2240)
                     /locus_tag="PC621_003538"
     CDS             complement(1560..2240)
                     /locus_tag="PC621_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF4110649.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
     gene            2500..2799
                     /locus_tag="PC621_003539"
     CDS             2500..2799
                     /locus_tag="PC621_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110650.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            3123..4778
                     /gene="ilvD"
                     /locus_tag="PC621_003540"
     CDS             3123..4778
                     /gene="ilvD"
                     /locus_tag="PC621_003540"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF4110651.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
BASE COUNT         1981 a          543 c          758 g         1715 t
ORIGIN      
        1 taattatcta gggaaatttt ggataatcaa aatatatttt ataaaatcaa aatacaatta
       61 cataaacttt attaaaaatt tgttgggaat ttctgactag cattaagaac gtaagtttgt
      121 tgggggatgg tatatgtgga aaagtcaaac actactgtta caaaaaaaga aaaaattgaa
      181 gatataggaa tgaaattaga acatttattg agtgaagata ttacttttta tgaattatta
      241 cttgagattt tgtataaaga aaaagaagag aattaattct cttctttaag gaatcttttt
      301 aaataggcat tatcaatcat tttttctata gcttctctat cttctttttc aagagaatat
      361 attttttcta agagcttttt tgttttttca cttactgata agttctctat gattaattta
      421 tcttctttgc ttatttttgt attatcattt acgcctaata aataatctgt tgatgtttct
      481 aatgcttttg ctaatctaac tattatttca cttttaggtt ctcttaaatt attttcataa
      541 cgtgacaagc tagcctcagt tataccgact ttagtagcaa gttctttttg atttatatct
      601 aattcttttc gtagatttgc tattctatct cctaaacttt ccatatgtac atcactcctt
      661 accgaaatta taagttattt tgataaaaag taaaataaaa ttaccaaaaa tataaaaaaa
      721 aagggttaaa ataataaaat atattgaaaa ttaccatttg tatatgtata atatgaatta
      781 tacaaatggt aatttgatga gaggtggtat gttgaaataa gaaactgaat taattaataa
      841 atatatagaa agttttgaaa acccttaatt gagatataaa caatatgtac aaaatttttt
      901 gcaagtaaaa ttaccaaatg tataaaataa atcagtagca tagataattt tatgctttaa
      961 tacattagat tatttaattt agttataatg gtcaaaaatg caatctacaa ataaaacaaa
     1021 gtattatcaa aaaagtattc tttaagagaa ttttttatta gagatgtaat ttatataaat
     1081 aatcgtatta aaaaataaaa tactaagttt aaaaatttaa ataatctata aatcaattat
     1141 aaaatattat ttcttagaaa ttaaaatgaa ttaagtaaat ggaggaattg atatgataat
     1201 aattagaagt caagataaaa ctaatttaat gcagataaac caaattaaaa tagatggtag
     1261 tcaagtttat gcagtatttg aaagtaaaat tgatactgta aaaataggag attatgaaaa
     1321 taatacaaga gctatacaag tgttggacaa tatacaaaac ttcattgaaa atggaacaaa
     1381 atatgattat ataacatcaa ataaagtcag atacaatgtt aataaaatat ttcagatgcc
     1441 agctaaatag tgagtgtgca ttttagttta ataactaaag cccactttct tttgtagtat
     1501 gaaagagggc tttacatatt tgtaaatatc gaagtacact tgttttatca ataaattatt
     1561 taataaggta taatggacca attaaacaat aaaaattctc ctaaaacagc aagtattaaa
     1621 tatataatag cttcattttt gtaaagagag gtaatttttt tgcttatgtc atcatcaatt
     1681 gtaatagcaa tttcatcttg aataatattt ttatatatag atgggaaaat atcttttttc
     1741 tttataagat taaataaatt atttacacct acccatataa aatatcctaa aacagcacct
     1801 aatgtgttca tcattaaatc atcaatatct gtagctctta agttaaacaa ttgacaaagt
     1861 tcaattgcta atgaaaaacc taatccagtt aatgaaacat tgataatatt tctaaaattt
     1921 ttccaaatta aaggtagtaa aaatcctaaa ggcataaaca taattatatt taaaatatat
     1981 gttaagatgc caactgagtc aaaaggtatt agatttattt catcaattct aaaaagccca
     2041 ctataccttc ctatatccca tatgctacca attccagctt tactaagtgc tagatagata
     2101 tataataaaa aaatatatac ccatactaaa tgtatcatta cattcttttc aactttcaaa
     2161 ttttttcgtt ttataacgac tatttggtat attaagcatg gtaatataac gcaaataaat
     2221 gaataaaatg tccatatcat tttattttgt cccctttcaa atttatgttt ttagttttat
     2281 aagtactatc tttatattaa actgtaagtt ttaaaaatct tttaataaaa tcttaaaaat
     2341 ttattaattt ataaaaatat attattaaga ttaaaaaata tatataatca aagtttctac
     2401 aattatatag ttaggtatat aaaaatttta tcgtatgtat aaatattata aatatatgga
     2461 aataataata gtatattttg gtaaagtagg aggaatttta tgaaatggat taaatggtat
     2521 agtattacat gcatatgtat ttttatagta gtagcttttt atatgtttat atttcctaat
     2581 aaaatagaaa ctattgatac ttcatctgca tatagctttg ttgaaaagaa agtaccaaat
     2641 agtgctgtat atcaaggata caaaaagaat ccagttgatg gtactactac aatttattac
     2701 agctatgata attcaactca tatagttaga ttatcacatc cagaagacta ttcaaggaag
     2761 attaattggg ataaagtttc aaacatcaga tttgattaat ctttaatatt tataaaagtc
     2821 tatatatcta aggcaaatat attattaatc ttattcatag ttgagcactt tctattatag
     2881 aagtgcttct tctgatttat atcataaaat attactcaac aatttgtaaa atatattagt
     2941 ttataaaata tattgaaaag ataatattat ataatacgat tgtgtaagat attgataaaa
     3001 aatcgtaata aattttcatt agtatatgct aatatttttt tacaattttc gtaattaatt
     3061 aatagaaaaa tatattataa taatattatg aaaatttaaa aaagtaagaa agaggtaata
     3121 ttatgagaag tgatataaaa aaaggaatag aaggagctcc aaagagagcg ttaatgtatg
     3181 gaatgggtct tactaaagaa gaaattgaaa gaccattaat aggtatagta aatgcacaaa
     3241 atgaggtgat tcctggtcat ctgcatcttg atgaaatagc agaagctgca aaaaatggag
     3301 taagaatgag tggtggtttg ccattagaat ttcctgctat aggggtatgt gatggtatag
     3361 ctatggggca tgtcggaatg aattattccc tggcttcaag agaattaata gcagattcaa
     3421 tagaagctat ggctatggca catggttttg atgctttggt acttatacct aattgtgaca
     3481 agatagtgcc aggtatgtta atggctgctg caagacttaa tataccaagt attgttgtga
     3541 gtggagggcc tatgcttcca ggtaaaaaaa atggaaaagt atatgatttt aactctgcaa
     3601 tggagggtgt tggagcatgt aaagatggaa cagtaagtga agaagaatta gaagagttag
     3661 ccatgaactc ttgtcctgga tgtggttcat gctctggact tttcacagca aatagcatga
     3721 attgtttaac agaagcttta ggcatgggta taccatataa tggtactgct gcgtcacatt
     3781 ctggagaaag aaaaagaata gcaaaatatg caggtatgta tgttatggag ttacttaaga
     3841 acgacataaa acctagagat attttaacaa tagatgcttt taaaaatgct atagctgttg
     3901 atatggcaat ggctggttct acaaatacag tacttcactt acctgcaata gcttatgaat
     3961 caggaataga gtttaactta gatttttttg atgaaataag tgaaaaaact ccttgtttaa
     4021 caaaattaag tccaagtgga aaacatcata ttgaagattt acatatggca ggaggaatac
     4081 cagctataat gaacgaactt tcaaagataa atggaataaa tttagattgc aaaaccgtaa
     4141 caggcaagac tataggggaa aatataagaa attgtgaaat agaaaatgaa gaagtaatac
     4201 atacattaaa gaatccatat agtaaccaag gtgggcttgc aatattgaaa ggaaatcttg
     4261 ctctaaatgg agctgttgta aaaaagtcag cagttgcaga agaaatgtta gttcatgaag
     4321 gaccagcaag agtttttaat tcagaagaag aagctgtaaa tgctattttt ggtaaaaaaa
     4381 taaacaaagg tgatgttata gttataagat atgaaggtcc aaagggtggt ccaggaatga
     4441 aagaaatgct atctcctaca tcagcagttg caggaatggg acttgataag catgtagcac
     4501 ttcttactga tggtcgtttt tcaggagcaa ctagaggagc atctataggc catatttctc
     4561 cagaagctat ggaaggtggt ttaatcggac tagttgaaga aggagatata atttctataa
     4621 acataccaga caaaaaatta gagttaaaag tagatgaagt tgaaatagaa aatagaaaat
     4681 taaaatttaa acctttagaa ccgaaaataa agcatggata cttaagtaga tatgctaaat
     4741 tggtaacatc agcaaataca ggagcagttt taaaatagtg aactatattt acgaaaatta
     4801 gaaaaaatta agaaaatatt aattggatat cttaggtgtt atagcactaa tgaggtatcc
     4861 tttttatttt gtatgaaaaa tatactgttt gtatacaaaa atattaaaga tattttagta
     4921 agttttgtat ataagcaaac atgtattttt taatatagtt atctaaatat tattttatat
     4981 acaaaaaaat atgactc
//
LOCUS       DAMKPG010000168         4963 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMKPG010000168 DAMKPG010000000
VERSION     DAMKPG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.174"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(431..2566)
                     /gene="rnr"
                     /locus_tag="PC621_003541"
     CDS             complement(431..2566)
                     /gene="rnr"
                     /locus_tag="PC621_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF4110652.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKIKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3162..3776
                     /locus_tag="PC621_003542"
     CDS             3162..3776
                     /locus_tag="PC621_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF4110653.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3794..4156
                     /locus_tag="PC621_003543"
     CDS             3794..4156
                     /locus_tag="PC621_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110654.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4262..4759
                     /locus_tag="PC621_003544"
     CDS             4262..4759
                     /locus_tag="PC621_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF4110655.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1620 a          751 c          601 g         1991 t
ORIGIN      
        1 attagccact tctattaaac atttcttaat tacttatttt tcaattagcc acttctatta
       61 aacatttctt aattacttat ttttcaatta gccacttcta ttaagcattt cttaattact
      121 tatttttcaa ttagtcactt ccattaaaca tttcttaatt atttattttt cagttggctt
      181 tttctattaa atgtttctta attacttatc tttcagttag ctctttctat taaatatttc
      241 tcaattactt atttttcaat tagctacttt cattaaatat ttcttaatta tttatcttta
      301 agttaactat ttccattaga tatttcttaa ttacctattt ttatcattta ttttatagag
      361 tattgtgcac tatatattta tacatatcat aaatactaca acttgttatc tcttatttta
      421 gctgttttat ctactcctct attttttcaa ctatctcaaa gtcaatttct ttaaaatcta
      481 cattaacact ttcaaccttt atcttaacaa ggtctcctat cttatatgac ttcttagttc
      541 tttctcctaa aacactataa gaactctcat cataaatata gtaatcatca ttcatatttg
      601 caagtcttat caatccttct atagtattat ctaactcaac aaatattcca aatgaagtta
      661 cgctagatac aacaccctca aattcttgac caactttatc cttcatataa agagccttat
      721 agaaatcatg tacatctctt tcagctaact ctgcctttct ttctttctcg gatgattgaa
      781 cagatgcata atccactata gtagtaagct gttcttgtct ttttccactt attttattat
      841 ttaaagattc ttttattatt ctatgaattt gaaggtcagg atatcttctt attggtgaag
      901 taaagtgaca gtagaattta gctgctaatc caaaatgtcc actacattct ggtgagtact
      961 tagcttgttt taaagaacga agcattatag tactgatagc tccttcttct tttttgccct
     1021 ttattttctc tattatacct tgtaatgctt ttggatgtac ttcctcttta tcacctttta
     1081 ttatatatcc aaatgtagat ataaacttac tcaattcttc cattttttcc aaagatggtg
     1141 tctcatgtat tctatacaca aacggtattc ccatccagta aaaatgttcg gcaattgtct
     1201 cattacttat aagcataaat tcttctatta tcttattaga tattcttctt tcatatggct
     1261 ttatatctac aacttcgcct tttccattta gaattatctt tgcctctggg aaattgaagt
     1321 ctatagctcc tcttttttct cttcttgaca taagaatctt agctaaaatt tcagcatttt
     1381 taaaatcttc aacaacatgt tcaaaagtct ttttaatttt ttcatcatcc ttttctaata
     1441 tatcagatac ttctgtataa gtcattctag cttttgaatt tattacagat tcacatattt
     1501 catgtttaac cacatttcct ttatggtcta tttccataaa tatagaaaga gttaacttat
     1561 cttcaaatgg atttaagcta catactccat ttgaaagagt ttttggcaac attggaatta
     1621 ctttatctac taaataaact gatgtagctc tttttaaggc ttccttatca agtttatttt
     1681 tctctcttac ataatgtgta acatcagcta tgtgaactcc tagtttgaag tttccattag
     1741 gtaatacttc tattgatatg gcatcatcta agtcttttgc gtcatctcca tctatagtaa
     1801 atatgtttaa atctcttaaa tctcttcttc tttttatctc ttcttgagga atctcaacag
     1861 ctacagcttc tgcttcttgc aatactttct ttggaaattc ttctggaaga ccatgttctc
     1921 ttattataga atctatttct acgcctcttt cacctttttg acctagtatc tctataattt
     1981 tgccttctgg ttttctatcc tcttgtggcc atactgtaat ttcacatact accttatcat
     2041 tatctactgc tccactaaaa tgttttttag gtatatatat atctttagta aatttcttgt
     2101 tgtctggtgt tacaaatcca aaagttttac ttgattggaa agtacctact attctagtta
     2161 tttctctctt tattactttt actattcttc cttctgctct cttttcttca gttgcaggta
     2221 ctactatttc tgccataact ctatcgttat gcatagcacc atttatattg tcacttgaga
     2281 taaataaatc ctgtgtatat tcaacatcag attctacaaa tccaaaaccc tttttgtgtg
     2341 atacaaattt tccaacaaag tatcccattt gattgggaga tactattttt ccttttttag
     2401 taaagcagat ataaccatct tcttctaatt catttaaaaa attataaaac attggcattt
     2461 ctgatgaatg tatatcaaat atttctgcta attcttcttt ttttagagga ttatatgcac
     2521 tttcatttat taatcctagc aaactttctt taagtcctgg tatcattttt ccctcctgtt
     2581 ttacttttca ttatattatt atttatattg tcttttatat ttcttcagat aaaacatttt
     2641 ttacttatca cataattttc tatattttct tattattatt acaagttaaa attacttatt
     2701 tatatttttg atttaatgta agctttgtca tactaacttc aaacaaatta tgatgtttta
     2761 taaattattt gtaaatatca ttataattac ttaagttatt ctttatcata attgcttaaa
     2821 ttactcttta tctcataatt atacaccatc aaataggatt ggtaaaatat tcatctcata
     2881 taagtattac tatataataa tttattctat tagtatactc aaatcatgta aaatttatat
     2941 aactcaatta attttttaaa tacaacttta taatataaaa cttataataa aagtaaattt
     3001 atatgttaaa tgaggttgat tttttattat tttttattta tgctagtact aaagacttgc
     3061 aaacgttttt tgacaagtct ttattttgtc taaattaaac aaactttttt acaaataaat
     3121 acataaaatt taaactaatc tatttattgg aggagataaa attggaagaa gtaattgcaa
     3181 aaattattac tatagaaaat ggattcaaag aaattgaaaa cattgctaaa aaaattgtaa
     3241 aaaataatga ctcaaaaaag tgttattatc tatcaatcta cttatatagg tcagaatatt
     3301 atcaagtaag agaattggct gtgtttatac ttggttttat atcagtaact atttctgaag
     3361 ctttgttatt tttaaaagac aatgtgagta aagatgaaaa ctggagagta caagaaatac
     3421 tagctaaatc atttgattat tattgcagtg aagtaggata tgaaaaggca cttcctgtta
     3481 taaaagaatg gcttaattct gatagtccaa acataaaaag ggctgtgaca gaaggtctta
     3541 gaatttggac tagtagagac tattttaaat ctaatcctga cgttgctata tcactacttt
     3601 ctagtttaaa agaagatgat agcgaatact tgagaaagtc tgtaggtaat gctcttagag
     3661 atataagtaa aaaatatcca gatttagtaa aaaaagaatt agattcatgg gatatatcaa
     3721 ataaaaaagt tgagtttgta tataagcttg cttcaaaatg tatcttggca aagtaaaatg
     3781 gaggtgaact tatttgaaac ttcgtgatga gtatacttgc ccattagaac taacacatga
     3841 tatgataaaa ggcaaatgga aatctattat attatggaga ttacgcatag gaccaacctc
     3901 cttatcaaaa cttgaaaagg aaatcaatgg tattaatcaa aagatgttac ttgaacaact
     3961 taaagaatta attgaatttg gtcttgtaga taaaaagaca tataaaggtt acccacttaa
     4021 agttgaatac tcacttacac ctattcgagg gaaacaaata ttggatgctt tggctataat
     4081 gcaagatata ggaattagct ttatgttgga taatggtatg gaagatattc ttaaggaaaa
     4141 aggtgtttta aaataaatag atacccacaa aaaagtaagt aatttaccat tattttcaac
     4201 tggtatataa ttaaattata gatatattct attaatttat tataatagga ggtaaattat
     4261 tatgaaagta actagtacag gtatagtaaa tggtgttata gaagataaat acggtaaaag
     4321 aggaactcag ttcaatgaag gtggtatgcc tacatattca ctacctctaa agtttgaaga
     4381 tgctccaaag aatacagtat cctttgctgt atttttagaa gataaagact ctgttcctgt
     4441 atgtgggttt gcatggatac attggttagc tgtaaatata acaaaagatg agttaaatga
     4501 aaatgaaagt gtaacagcta ctgattttgt acaagggact actagttggc atggcaaact
     4561 aggaggtctt gacagattag aggccagtgt atatggtgga atggctcctc cagacagacc
     4621 tcatgtctat gaaatacatg tatttgcact tgatactatc cttgaccttg aaaaaggatt
     4681 ctatatgaat gaatttttca aagctattga agggcatgtt ttagatacat ttacactaaa
     4741 aggaagctat tctaattaat taggtttact atagctaatc attaattaaa tgctacattt
     4801 aattgatgat tagcttaatt gttattagaa tattttctgc actttatctt aatatatatt
     4861 ttagttgact ggtattttaa tttctattag attattatca atttatataa attgatttta
     4921 gcttaaaact tacttatcta tttcagtttc tattagaatt att
//
LOCUS       DAMKPG010000169         4889 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMKPG010000169 DAMKPG010000000
VERSION     DAMKPG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.175"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            41..844
                     /locus_tag="PC621_003545"
     CDS             41..844
                     /locus_tag="PC621_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110656.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            856..1986
                     /locus_tag="PC621_003546"
     CDS             856..1986
                     /locus_tag="PC621_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF4110657.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            1990..3399
                     /locus_tag="PC621_003547"
     CDS             1990..3399
                     /locus_tag="PC621_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110658.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3610..4119
                     /locus_tag="PC621_003548"
     CDS             3610..4119
                     /locus_tag="PC621_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF4110659.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2035 a          417 c          854 g         1583 t
ORIGIN      
        1 aatatagata taaaaataat tatagaaaga aaggcacatt atggaaaaat tgagagttat
       61 tgaaatgata aaaaataatc ccaatattat agctacaata gataatccaa ctgatgaaat
      121 gaaattgtta gctattaaag aaaatgggct tgtactagaa tatataaata atccaaccag
      181 agagatgcaa gaactagcaa tagataataa tattagagca attaaattta tagataatcc
      241 aacagaagat atgatgataa aagcagtaaa tgagggatgg agtatactgg attatatcaa
      301 aaacccaact gataaagtta ttgaaatggc tataaatcaa gcaggatggg caattaaata
      361 tgctaaaaat ccaagtgaag aattacaact gttagcagta agaaaaaatt atgattcgat
      421 aaagtttata aaacaacctt atgaaagtgt acaagaggag gcagtaagaa taagttatga
      481 tgccttgaga tatatagatt cacctagtta taatgcagaa cttatagcta taaaaaataa
      541 tgaagcagct attagcttta taactgattt agataaaaat aaaatgttgg aatttttaaa
      601 agtgaatatt ttagtgatta agtacattct gaaaaaagta tctaaagatg aattagagag
      661 tgtattaaaa gaagtattat ccaaagaaga tgttgaggaa aagtatatta gagatttttt
      721 gaattgtagt gttatagata gaaatagcga aagtatacca atagataaaa ttatgtttat
      781 ttataaatat ggaagtaaga cagcaaaaaa aatagctgta gatgagaaat taaagattat
      841 gtaggagaga gaaaaatgaa ttttatagac acattaaaaa gcgttgactt aatattatcc
      901 actggagaag tacctttaat agtaggtgaa agtggaatag gaaaaacagc tttagcaaat
      961 aagcttgcca aagaaaatga ttggagttta attgttattg atggaaactt acttaaagag
     1021 ggtgaaatag gtggtcttcc aactatagaa tcttatgtag gagttaattc taatggatat
     1081 aagactgaaa agaaaacaac agtgtatgct gttcataaca aattaagaga aattgatgaa
     1141 gagatatcta aaagtaagac agttctttta tttatagatg agataaatcg ttgtgagcat
     1201 acagtacagc aagaacttat gaatttaatt ttaaatagag aaattaatgg atataaactg
     1261 catgatgatg taaagatttt agcagcaatg aatccatcaa gtaaatatgg ttcagatttt
     1321 gattatcaag tggtagatat ggatgcagcc caagaaaata ggtttgtttg gttaaatatg
     1381 gagagtgacc atacacaatg gataaaatgg gcaatagatg agggtattga aagaaaagtt
     1441 atcgagttta tttccacttt tccagaatat ctgcataaaa taaatgaaga tgatgtaaga
     1501 gctactccta gaagttatga gagagtatct aaaatttata aagtttataa agaaaaaaat
     1561 aattctattc ctagagcagt atttttaaat gttgtaaagg gtaatgtagg taaagttatt
     1621 gcagaagaat ttataagttt tattgaatca aactctgaac cattaatatc ctatgaagat
     1681 gtttttttaa gagaatctat tgatgagccc attgtagaaa gagtaaaaaa tgagagtcat
     1741 acaagacttt atctatcagc tatgaacatt ctaaaagact tagaattaaa tattaagaat
     1801 gataaatatg aatcaaacca ctatattaat agatttattg aatttttaaa aatgtatcct
     1861 gtagatttaa tgataggtat tatgaaggat attagaaaca gttatattga agtatataaa
     1921 aaagccatag aaaatgaagg ttttgtaaaa tcatattttg aatcttatag cttaataagg
     1981 ggataatgta tggaaaatta ttttgataaa caagcaaaag agctttataa taaagcaagt
     2041 gaaattataa atactcattc tatgctaaag gcaaatcgtg ctaatgagaa atttgatatg
     2101 gatataccac aagattttaa gtatgagttt tttagtcttg tagataaagt gaatttaagt
     2161 cttatggaag aagaagataa tttttatgga tattttttgt ttcagatgtc aagagaaata
     2221 agatttgata taagtagtcc tacaggcgtg aattttaagg gagctaaata tgttatatat
     2281 tttaatccaa taatcttttt aacgcttgac ataaagcaaa tggagactac aattaaacat
     2341 gaaattcatc atatattatc tatgcatttg atgagagcaa aggagttaaa aggaaagtat
     2401 agtacattgg caattaatat ggcaatggat atagtagtaa ataaatttct aaacaattta
     2461 ccaccatatg caactacctt agagtgggta aatttaaaat attccttaaa acttgaacca
     2521 tatgaacctt ttgaatatta tgtagaaaag attcagactg aattagattt gatggaagtg
     2581 gatgaagatg gtgaggaaga tgatagtgat aaatatggag atgtagaaat tgattatagc
     2641 cccgaaagaa ctcatgatat atgggatgaa tccaatgaga tagataaaaa tacccttaga
     2701 gaatttactg aaaaatttat taacagttct caaaaaggta aagttcctgc ttatttagaa
     2761 agtatgatat catcacttaa aaatagtaag ggtgaattgc catggaattt atatcttaat
     2821 agaataatgg gaacagttga aaataataaa aagaagacta taacaagaag aaatagaaga
     2881 caaccaaatc gtttagattt gaggggagaa cttagaagtc ataaggcaga aatagcagtt
     2941 gcacttgatg taagtggaag tattagtgat gaagaattta aacaagctat taaagaagtt
     3001 cttagtatag tgaaaaacta taatcatgaa attacaatta tagaatgtga taatgaaatt
     3061 agacgtgtat ataaagtgag aactgaaaaa gatataaaag atagaattag gataggtgga
     3121 ggtactagat tttctccagt ctttgagtat gctaataata aaaagattaa tttgttagtc
     3181 tactttactg atggtaaagg tgaaaataaa ctacaagtaa tacctagagg gtataaaatt
     3241 ttatgggtta tttctggaag aggagataaa atttcattaa aagaacctta tggagcagtt
     3301 aaaaaactta ctaaggttaa aacaaatgat gaaatattag aaataaatga tgtaagaaat
     3361 gatgggtact caatgaatag ccaagcacca attttatagg tattcacagg agataagctg
     3421 tattaattta ttttgatatg cttgttataa aaatctaaaa aatacattaa tcaatggtta
     3481 atgtattttt taattttaat aatataagtt ttacttacat ttaagtctta aaatgtgtta
     3541 aaatataata tatagcagat agttttggca agatggattt agttgtcaaa actatttttt
     3601 ttattaagga tggtgatgtt tttgtttaag attgatgatt atataatgta tggtatgaca
     3661 ggagtctgca aagtcatgga tataacaaat gaacgattta caaatggaat aaaaaaagag
     3721 tattatgtat tgagtcctat ttattctaat aatacagtaa taaaaattcc tgtagataat
     3781 gaaaaagtac caatgagaaa gttactttct aaaattaatg tgctttcatt aataaatgat
     3841 attcctaata tggatacatc atggatagat aatgaaaaat taagaagtga gcaatttaaa
     3901 aaaatactca gaggtggtaa atgtgaagag ttaataaaat tagtaagaag tatagataat
     3961 aacagagagt atgtaaaatc tattggtaaa aaaacacatc aagcagatga taatataatg
     4021 aaagaagcag aaaggttatt aagtgaagaa tttgcaacta ttttagatat ttctcctaat
     4081 gaagttagtt catatatttc aagtcatata cctcaataaa gtgttttagt atcctttatt
     4141 taatctattt taagtattat ttatgtatgc tggttttggt aaaataaaat atataattaa
     4201 attatataaa aaattaagaa tgattgtatt gaagaattac agaatagtgc ggagagccca
     4261 ctcctttagg tgtgagatgg atatcactta aagatttaaa ttgttattgt agatgttttt
     4321 aatataaagc aagattaata agtgtattgt aagctttaag tgaaattgat actataatga
     4381 gttacttaga ttttaaaata tataatatta taaaaaatcc ttttaggttt tgaatgaata
     4441 agatggatac tatgttgaat agaatccatc ttatttaagt tttatttatg ttgtttattt
     4501 gtaagcatta tttttgtaca tgaaaacaca gtgtaaaaat atgcttagaa ttatgaagtt
     4561 ttattaaaaa tatattcgac aaaataagac taaaaatatt cttaaactta taactaagat
     4621 aaatattgta tatttatagt gtagcctgaa aaattgtgga tgtacgaaat ttatttagta
     4681 ttttattgaa acaaagtaga agaagtacta tagtttatct ttgaatagat tgttattttt
     4741 tagtgaaaaa gttattaggt taatacataa atataatagc ttataaatag tttaagcatg
     4801 ttaaaaaagt agttattaga gatatatagt tacattgctt ataagatact taaaatgata
     4861 gcattcactt atatttagaa aattaagat
//
LOCUS       DAMKPG010000170         4835 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMKPG010000170 DAMKPG010000000
VERSION     DAMKPG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.176"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            141..802
                     /locus_tag="PC621_003549"
                     /pseudo
     CDS             141..802
                     /locus_tag="PC621_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1021..2058)
                     /locus_tag="PC621_003550"
     CDS             complement(1021..2058)
                     /locus_tag="PC621_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF4110660.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2051..2737)
                     /locus_tag="PC621_003551"
     CDS             complement(2051..2737)
                     /locus_tag="PC621_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110661.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2749..3069)
                     /locus_tag="PC621_003552"
     CDS             complement(2749..3069)
                     /locus_tag="PC621_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110662.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
     gene            3430..4770
                     /locus_tag="PC621_003553"
     CDS             3430..4770
                     /locus_tag="PC621_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF4110663.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         1785 a          532 c          721 g         1797 t
ORIGIN      
        1 taataacaat tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg
       61 tggagaatta gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac
      121 tttagtcatg ggaggttcag attttggctg aagatgaaga aaataatcat ataaatattg
      181 agatgcaaag agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg
      241 tacatggtag aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa
      301 tacatatact aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat
      361 taacaatgga cgagcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc
      421 ttccaaaaat tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta
      481 ttgataatcc ttctagctca gatgtaagag ttatagctaa gaatgattct attattaaaa
      541 aagcacttga tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa
      601 gaggtgctta tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag
      661 caattagagt tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaatggttt
      721 taaattagat gacatattga ataaatttaa tgattttgac ttaagtgaag atgagataaa
      781 agaagtatat gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat
      841 ctgagcctgg aaatatttat gtgtattttt taatgaataa aaatttattt taaaaacaat
      901 aattagaggt acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta
      961 gatttattat ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat
     1021 ctaatgattg ttttttataa tagttaaatt tccatttgta gtgcttaaat ataaatttgt
     1081 aataccattt ttataagtgt ataaatcact atgacgattc aactcaaagt cactgtccaa
     1141 aacaccagaa accttatcaa aatttaaagt gaaacctgga ttatcaggaa tagacaaatc
     1201 gacattggca gaagtagttt cacagtacag agaagacaac attttgctag aattgatttt
     1261 tatattactg ctagttgact cagctttaat gtcagaaaaa actccagata agttcatttt
     1321 tccagaaact gaactacaag taagattttt attgtatata tcttttaaat ttatattgcc
     1381 attagttgtt tctaagttta aaacatcaat tttcccagaa agctgtattt caccagatgt
     1441 tgatttgagt gtagcatttt tactatttag ttttgttata tcaatgtgac cactagttgt
     1501 gtctaaattt attttattaa tattaccaga gagacctatt gcacctgaaa ttgtgttagc
     1561 ttcaactgta ttacttctta tgtaagtgct atatatatct gctgaagtag tatataaggt
     1621 tattttatca taaagcttat tgggcaaata tagttcaagt attgttggtt ttggaagatt
     1681 aattccaatg aagaattttt tagttgaatt atcatatata gaaagagaat ttccgtcaac
     1741 cattattttt gctagttttt tctttgagaa attactaggg attttttgaa ttaatttgat
     1801 tttattagaa tcacttctct taattctaac ttcacctgaa atccaatcaa cattaatgtc
     1861 atttatttta tctgaaaaac tttcattttt agccactata tagttatcat aattgtattt
     1921 aggattaaga gtattaaaag ggaaattaaa agaatgtgaa attatagttc ctataaggag
     1981 tgagataaat acaatagaaa gtgatattgc caggatactc cagatgaaaa tctttgcctt
     2041 tataagcttt ttattcatat ttatcctcct ccttgtagcc ttttagttgt ataattcctt
     2101 tgacaatgtt gacaccagca gcacctatta taaatagtat ccacgaaaag gaccaaatat
     2161 taaatagtat gcttgtgatt agatatatag aaactactat aatccaatat gcattcataa
     2221 atgatttata aactggattt atatgattat aattttcctc aaaatcctca aattctttga
     2281 atttatttga taaattatca ttgtatagag catagtttgt ccttgtcatt tgattataga
     2341 ctaataaacc tactccaagt gctattaata tgagcattaa aagaatagct atagttggtt
     2401 ctatgccaaa acttacaact gagactacaa ataggaaagg gcttattata aaaagagcaa
     2461 ctgctattga tgctcttaag gctcttttct ttttttcttc aattaattca gaaggtagag
     2521 aatatggttc cttaacactt tcaattaact cagataaatc accaattcct gagattactg
     2581 agttatatgc ttcttgttct gttttaccat cttgtatttg gtcgttatac ttttctttta
     2641 gattttcaaa taattcatct ttaagttctt tgactttttt tgtctcatta actcctgtaa
     2701 agctactgtc aatataagta cgtattttgt ctaacatatt ttcacctcct aaattaatat
     2761 atctatcaat gcttttgtat tattccattc ttcacggctt tttatatatt tctctttacc
     2821 tatgtttgtt attgaataat actttcttct agctccagta acttcatcac cccaataaga
     2881 ggttattgca ccagattgtt caagtctacg aaatgctgta tacaaagtag cttctttcaa
     2941 ctcatacaat cctttggttt tttcaagtat agatttatta atctcataac cataactgtc
     3001 tttttcaagt aaatgtttta agataattgt ttctgtatga cctcttatca tgtcagatgc
     3061 gattatcatg aaaatcacct ccaattaaat atataatact ttgatatact tcgcctgtca
     3121 aggtatatca tgagaaaaga cattttattg taatatttat aaggttttat atagaaataa
     3181 tgtaaaaact ttataaataa tatattttat acctagttat agataacttt atatagttat
     3241 tattaaaaaa agcaataaaa agtttaattc aatttagcaa atttagtgat ataattaaaa
     3301 aagaaaagaa atatggactt atttggaata attttgacaa tctatttact acacgtgtag
     3361 agtcgtatat aattaaatta tacataaaat ataaaaaaat aatttagtaa tacgttagga
     3421 gaaagtatta tgataaagtc aagcttattt tggaatattt tcattgcgat atcctttatg
     3481 atagaatggt ttctttgtaa gaaaatgcta gattatacaa gtgagaaaag gatttctaac
     3541 aaaaaaataa acttttgtat ggtaggtatt attgtttttg ctgacataat atattttttt
     3601 gatgtacctc caaatataag agtaattata tctatgataa ttactattgc attttataaa
     3661 atctcatatg atgtgggatt tataaagtct gtattaatat ccttaattta ttggatgtta
     3721 ctaatcgcca cagatgcttt gagtatatct ttaacagttt ggcttaattc tataaatgac
     3781 atgagtagta tggggacggg agatatatac agaattgagt ctataatatt gggaaagata
     3841 cttttgattg tgtctttgtt tttatataga gccttaaaga taaaacttaa tatagaaata
     3901 aataaaagga cattatttta tatagttatt cctatagtcg ctaatatagc atcttttttt
     3961 gtaatttttg aatacatatt taatccaggg tctggtagct taatacataa tttccaattc
     4021 gttatgattt ctgttgtatt gcttatatca agtatcactt tagttttaat tgctaaaaaa
     4081 ttacagcaat ataatacttt agttgtagaa aacaatatga ttaaaaatac tttgaagtat
     4141 gaaaatgagt attatgaaga tatagaaaag aaatatttaa aaactagatt actaagtcat
     4201 gatatgaaaa atcacatact gtgtataaat gctatgatta aaaaaggtat tgatgttact
     4261 tcttatttaa ataatttgca ggtagaaatt cagagtaatg acttgttatt taatactggt
     4321 aattttatat tagatgctat attaagtgaa aagaaagaag tttgtgataa gaatgatata
     4381 gaactaaaaa taggaataaa ttttagttgt tgtgaagctg ttgaattagt agatatatgt
     4441 catatatttt caaatatttt agacaatgca attgaggcat gtaataaaat tgatagtgga
     4501 aatagagtta tttgtgttaa aggtgatgtt atgcaaaatt tttatttaat aagagttgaa
     4561 aatactaaga tgaataagat tattactaaa aatggagata ttttgacaga caaaaaaggt
     4621 tctcttcatg gacttggaat aaaaagtgta aaaaactctg taaataagta tggtggtaat
     4681 gttgtaattg aatatgatga taaaaaattt gtagttaaaa ttgctattcc acttagaaat
     4741 gtgaagaaag cagttaatca tgtgatttaa atgtatataa atttgatgtg tagaaattta
     4801 atatataaga attaatgtat aaaagtgatg tatag
//
LOCUS       DAMKPG010000171         4546 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMKPG010000171 DAMKPG010000000
VERSION     DAMKPG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.177"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            202..1008
                     /locus_tag="PC621_003554"
     CDS             202..1008
                     /locus_tag="PC621_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF4110664.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="PC621_003555"
     CDS             1135..1407
                     /locus_tag="PC621_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110665.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="PC621_003556"
     CDS             complement(1570..2142)
                     /locus_tag="PC621_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110666.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="PC621_003557"
     CDS             2326..3168
                     /locus_tag="PC621_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF4110667.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="PC621_003558"
     CDS             complement(3271..4347)
                     /locus_tag="PC621_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4110668.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1658 a          588 c          710 g         1590 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtactcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agtaataaat taagtc
//
LOCUS       DAMKPG010000172         4535 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMKPG010000172 DAMKPG010000000
VERSION     DAMKPG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.178"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     misc_binding    120..354
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            404..1423
                     /gene="pheS"
                     /locus_tag="PC621_003559"
     CDS             404..1423
                     /gene="pheS"
                     /locus_tag="PC621_003559"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF4110669.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            1440..3833
                     /locus_tag="PC621_003560"
     CDS             1440..3833
                     /locus_tag="PC621_003560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF4110670.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            3858..4448
                     /gene="zapA"
                     /locus_tag="PC621_003561"
     CDS             3858..4448
                     /gene="zapA"
                     /locus_tag="PC621_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF4110671.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         1790 a          524 c          924 g         1297 t
ORIGIN      
        1 tgcattagat atatattata taaaaaataa ttaaattaat tatgtattgg atatatatta
       61 aaaatagtca cataatttga atggaaatga tataatacta aaataaacaa tataatattg
      121 taaatgcaat gaaagaggaa agtattttga ttaaacttag taaagagata aacacctagg
      181 ctgggagtgt tttctaagag gtcatgagaa gttccctctg gagtaacaga gctgaaattt
      241 tacagtaggc tttgacgtca aaaacgcgtt aagtttgtta gaggtggttt gatgattttt
      301 aaattgttaa actactaggg tggtaccgcg aaactatagc ctttcgtccc tagacaggga
      361 ttgaggggct ttttttatac aaaaaaacga aagggtgata tgtgtgcaag aaaaattact
      421 tgctttacgt gaagcagctt tggctgaaat aaaagaagca caaagcatag aaagtgtaga
      481 aagtttaaga gttaagtact taggaaaaaa aggtgagata actgccatac ttaaagaaat
      541 gggtaaatta tctgctgaag aaagaccagt agttggtaag gttgccaatg aggtaagaga
      601 aaacattgaa cttagcataa attctaaaaa agaagaaata aatgctattg aaaaagaaag
      661 aaaattaaaa gaggaagtga tagatgttac tcaaccagga aaagttttaa aggttgggaa
      721 gaagcatcca ataactcaaa ttatagatga agtaacagat atatttatcg gaatgggatt
      781 ctctatagca gaagggccag aagttgagac tgttgaaaac aactttgacg cattaaacgc
      841 tcctaaagac catccatcaa gagatatgag cgatacattc tatatcaatg atggggtatt
      901 acttagaact caaacatctc cagttcaagt aagaactatg agaagtcaag agttaccaat
      961 aaaagtaatt gcaccaggta gatgttttag gtcagactcg ccagatgcta cacactcacc
     1021 aatgttccat cagatagaag ggcttgttgt tggaaaagat gttactatgg cagaatttaa
     1081 aggaactatg gatatcttcg ttgaaaaatt gtttggttct gatatcaaaa ctaagtttag
     1141 acctcacaac ttcccattta cagaaccaag tgcagaggtt gatgttactt gtttcaaatg
     1201 tggtggtaaa ggttgcccaa tgtgtaaata tgaaggttgg atagaaatat taggttcagg
     1261 tatggttcat ccaaatgtgc ttagaaattg tggaatagac ccagaagttt acagtggatt
     1321 tgcatttgga gttggggttg aaagacttgc aatgcttaaa tacgaaatag atgatattag
     1381 attattattc gaaaatgata tgagattctt aaatcaattt taattaggag ggtatgtaga
     1441 tgttagtatc tttaaaatgg cttagagact atgttgatat agacatggat gtaaaagagt
     1501 tcgctgataa aatgacaatg acaggaacta aagttgaaac aatagattat tatggtgaag
     1561 aaatagaaaa tatattggtt ggaaagattt tagaaataaa acaacatcca aatgctgata
     1621 agttggttgt aactaaagta gatattggag ataaagttgt tcaaatagtt acaggagcta
     1681 caaatatatc agaaggagat tatattccag tagctgtaaa tggttctaag ttacctggag
     1741 gagttgaaat caaacagact gatttcagag gtgaattatc agatggtatg atgtgttcag
     1801 cagctgaact aggtatagat gaacattaca ttgaggagta taaaagaggt ggtatatata
     1861 ttttagacca cgaagattct tatgaattag gaaaagatat aaaagatgtt ttaggattaa
     1921 aagatgcttt aatagatttt gaattaactt caaacagacc tgattgtaaa tgcatgatgg
     1981 gtatagctag agaagcagct gcaactatag gaacaaaagt aaaatatcct gaaatcgaag
     2041 taaaagaaag tgacgaagag atagatttca aagttgagat agataatcca gatttatgta
     2101 gaagatatgt tgctagaatg gttacagatg taaaaataga accttctcca tattggatgc
     2161 aaagaagact tacagaagca ggagtaagac ctataagtaa catagtcgat ataacaaact
     2221 tcgtaatgtt agagcttggt caaccacttc atgcttttga tataaatcaa gtagagactg
     2281 gaagaatagt agtaagaaat gctaaagatg gagagaaact tgtaacatta gatgatgttg
     2341 agagaacatt agataaagat atgctagtta taacaaatgg agaaaaatca cttggtttag
     2401 ctggtgtaat gggtggtgct aactcagaaa taacttctaa tacgaagact gtactttttg
     2461 aaagtgccaa tttcaaacca gaaaacataa gaatgacagc taaaaaagtt ggtattaggt
     2521 cagaagcatc ttcaagaaat gaaaaagact tagaccctaa tcttgcagag atagcagcaa
     2581 atagagctgc acaacttgtt gaaatgttag gagcaggaaa agttttaaaa ggtgttgtag
     2641 atgtatatcc aaataaacca gaacctaaaa aattggtagt aaatcctcaa agaattaacc
     2701 acctattagg tgtagatgta ccaatggagc agtttgtagg aattttagaa tcattagagt
     2761 ttaaatgtaa tttggtagct aatgataaat tagaaataga tgtaccaagc tttagaacag
     2821 atatggaaca agaagctgat gtatgggaag aaatagctag aatttatgga tttgagaata
     2881 taccttctgt acaattagaa ggaaatacga cagcaggaat taaaacttca aaacaaaaat
     2941 tcatggatgc tttaaaagat aattcaactg ctgtaggatt aaatgaaata ttaacatatt
     3001 catttgtaag ccctaaggga gtcgataaga taagagtacc agaaggtaat gctaagagaa
     3061 actttgttaa attacttaac ccattaggag aagaaacttc tgtaatgaga actactttaa
     3121 taccaaatat gttagatgtt ttatcaacta atgtatctca taaaatagaa gaagtatctg
     3181 catttgagtg tggacatata ttcatcccac aagattcaga attacctaaa gaagaaaata
     3241 gaatgtgtgt aggtatgtat ggtaaagatg ttgacttctt tgcactaaaa ggaactatag
     3301 aaactatact tgtaaatgtc ggatttaaag gttatgaaat tgaacctcaa gataataata
     3361 ctaccttcca ccctggtaga tgtgctaaaa tagtgtataa taataaatat gtaggtacat
     3421 taggtgaatt gcatccagat gttatagaaa actacaatct aggtcaaaga gtttatgtcg
     3481 ctgaaataga tattgacttt gtatttgata attctgatag aacaaagaat tatgtgccat
     3541 taccaaaata tccatctaca tcaagagata tagctcttat tgttaaagat gatgtatttg
     3601 taaaacaaat agaagatata ataaaggaaa atggtcaagg attagtagaa agctacaagt
     3661 tatttgatgt ttacaaaggt tctcaaatag aagctggata caagtcaatt gcgtattcaa
     3721 taacttatag aagtaaagat aaaactctaa cagatgaaga tgtagctaag gtgcatgata
     3781 agatattaag tgaattaagc gaaaaattaa atgctaactt aagatcaaat taacaactaa
     3841 tgtcggggga ggttaggatg aacaaagtaa tggttaaaat ccatggcgct gaatatccaa
     3901 tggttggaga caaatcagag aagtttatga ttagtattgc tgattttgta gacaaagaaa
     3961 tggataaaat aacacgtcaa aacccgaaat tgagcttatc agttgctgcg atactaactg
     4021 cattaaatat atcagacctt ttatttgaat gttcagatga gaatgagaag ttgattaaag
     4081 ctaatgaaga attgagtaag aaggttggag catctaatga agagcttcaa ttagaaatca
     4141 agtctttaaa gcttacgata gcagaaaaag aagcagaaaa tcgtgaagct gagactaaga
     4201 tgaaagagtt aattgagatt atagaaaata aaaagcaaga aatattcgaa cttagtaata
     4261 cgacagaagg ttctagagca gagttagatg tttataaaaa caagattgag gagttaagta
     4321 ctcaacttga agaagcaaat gaaagagcta ctatagctga aaacttagct tctgagtttc
     4381 aaaacaaagc ttatgattta cagttaaaat gtacagggtt aaataatgat gcaaaaaatg
     4441 tggaataaac taaaagattt gctgtctctt gttttttgag acagcttctt ttaatgtata
     4501 aatataaatg ctatataaat atagctaatg tttaa
//
LOCUS       DAMKPG010000173         4458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMKPG010000173 DAMKPG010000000
VERSION     DAMKPG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.179"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            181..993
                     /locus_tag="PC621_003562"
     CDS             181..993
                     /locus_tag="PC621_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF4110672.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1082..1273)
                     /locus_tag="PC621_003563"
     CDS             complement(1082..1273)
                     /locus_tag="PC621_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110673.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1578..1784
                     /locus_tag="PC621_003564"
     CDS             1578..1784
                     /locus_tag="PC621_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110674.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLTPIEYKNIYN"
     gene            2093..3712
                     /locus_tag="PC621_003565"
     CDS             2093..3712
                     /locus_tag="PC621_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF4110675.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            4086..4280
                     /locus_tag="PC621_003566"
     CDS             4086..4280
                     /locus_tag="PC621_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110676.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         1669 a          518 c          622 g         1649 t
ORIGIN      
        1 tcactttttc aagaatgtta taattttttt attgtattct ttttgctata gtctatgaat
       61 aaatcaatag aaaatcttct aagttgcttt taaataatga ctttatggtt caattgtact
      121 aaaatagtat atgtaggtta caaataatct aataaatatt aaagtttgga ggtaatagat
      181 atggcagatt gtaattcatg tccatcaaag ggaaattgta atagtcaatc aaattgttct
      241 attgaaaata accctaacaa taaatttgga aaaataattg gtgttatgag tgggaagggt
      301 ggagttggaa aatcaactgt aactgcttta cttgctaata agctaaacaa aatgggatat
      361 aaggttggta tattagattc agatataact ggaccaagta ttcctagact tatgggtgta
      421 aaaaatgtaa aagcttattc tgatggctct tatatttacc ctgtagaaaa ctcaaataac
      481 ataaaagtaa tgtctataaa ccttatgata gatgatgaga atgaacctgt tgtttggaga
      541 ggacctttac ttggaggagt tgtaaagcaa ttttatactg atgtactttg ggaagaatta
      601 gactatttat taatagatat gcctcctggt actggagatg ttgccttaac ggttatgcaa
      661 tctattccta taagtggaat agttatggtt tcagtacctc aagatttggt atctatgatt
      721 gtatcaaaag ctgttaatat ggctaaaaaa atgaacataa atgtccttgg agttattgaa
      781 aatatgagtt atatccaatg tcctgattgt tctaaaaaaa tcaagttatt tgaaggagaa
      841 tctactgaaa agtttttaga tgatttagac ttagagcttt taggagaact tcctatgact
      901 aaagaaatta tagatattac tcataatgga gttacagaaa ttagtgatga tttagattca
      961 atattgacta atgttgttga aaaaattaaa taaataaaaa ttttataatt ttatatttat
     1021 caaaaaaaga ggcttgtatt tattttataa tcatacaaat aaatataaac ctcttttatg
     1081 tttatctatg ttttaaatac acaaaatatt cttctacttc atctccattt tttactttta
     1141 ctaagtattc attataatga gcattatatg gctgtgtcat tttgcattct attacatttt
     1201 ctatgtcttc taattctaaa agttctacat cctttaaaaa ttttctttct atgagttcca
     1261 ttaatctttc cataatatat cctcacttcc cctataatta agataaacta tatatcctct
     1321 taatacagag tttatctgtt tattatatta tatccaatat attacagttt ctaacatcat
     1381 tcataaatta ttttaacaaa tagagattga ttttttaata aatatgcctt gataaaatga
     1441 aacatagacc aactcttagt tttatgtaaa gaaaacttaa agaaaactat agattatatt
     1501 cacaaaaact actcagaaga tataacagta agtaatatat gtaatgaatt acaacttaat
     1561 aaaagttatt tttttctttg tttaagaaaa aaagtggata tacttttact aattttttaa
     1621 ataaagttag agttgaaaaa aacaaaaagt atctattaaa aaaagacttg tctattttag
     1681 acgttactgt actaattgga ttcaatagtc aaaattacta tagtataatt tttaaaaagt
     1741 ttaataacct tacaccaatt gaatataaaa atatttataa ctaaaaaata tcattctaaa
     1801 aggatgctat ttttttacat attttttaca attatagtta attctttgtt aaattgattg
     1861 tacataaaat actaaaaatc taagttcaag tatttaaatt gctatatatc ataaattttc
     1921 taaaattaac aacaacttaa aaatttaaca ttctcttaac gtatacttaa caaaacatta
     1981 atatttctat tgacaaaatt tgttattcta tattatggaa acaataaatt ttagttaata
     2041 aagtttataa ttcagataaa aaaactatta atttcttaag ggaggaaata tcgtgaatat
     2101 agtaattagc cttatcggtg gacttggact ttttctttac ggaatgagtt taatgggaga
     2161 aggtttacaa aaatcagcag gagacaaatt aaaaaaaatt attgaactct taacaagtaa
     2221 tgttgttatg ggtgtactgg taggtacagt cgttacaggt attatacaaa gttcaagtgc
     2281 tactactgtt atggttgtag gatttgtaaa tgcaggtata atgaatctaa gtcaagctat
     2341 tggtgtaata atgggggcaa atataggtac tacagttact gctcaattag tttcatttaa
     2401 tcttgaagga attgcaccaa ttgctttagg tataggtatt ttattctatt tatttacatc
     2461 aaatcaaaaa acaaagcatt tatctgagat acttataggt tttggtatat tatttacagg
     2521 aatggagttt atgaaagatg cagtagctcc acttgctgaa tataaagcat tcactgatgc
     2581 acttttatat tttagtaaaa atcctgtatt aggtatattg gcaggatttg ccataactgg
     2641 tattgttcaa agttcaagtg catctatggg tatgttaata gcactagctt ctcaaggaat
     2701 acttcctcta tcatctgctt tacctattct ttatggagat aatataggta cttgtgttac
     2761 ttcactttta tcaagtgttg gtgcaagtag aaatgctcga cgtgcagctg taatgcattt
     2821 aagttttaat gtcattggta caataatatt tatgcttgtt ttaaataaac ctatatcagc
     2881 catagttact cattttgacc caactgatac agctagacaa atagcaaatg cacatacttt
     2941 atttaatctt acaaatgtta taatattatt accattttct aaatacatag ttaaattagc
     3001 aaatagactt atacctatta aagaaactga atcagaaata gtaaataata ctaagtactt
     3061 agatgaaagg atgtttagca ctccatctat agcacttggt aatactgtac aagaagttgt
     3121 aagaatgggt cataaagcta caaactcttt ggaacattca attgcaggat ttttaaataa
     3181 gagcaatgaa gatattaata aagcttttga gtcagaaaaa gttgtcaata agttacagaa
     3241 agatatattg aactatttgt taaaactttc taaagaacct cttagagacg atgaacgctt
     3301 tagaacagat ttgcttttta atactgtaaa tgacattgaa agagtctctg accatgcaga
     3361 aaatatagct gaattggcca tgagtgttaa ggaaatgaac attagttttt ctgatagtgc
     3421 gataagagaa atatacgaaa tatacaataa gacaattact aattttaagg atgccttaat
     3481 agttttagat gttaaagatt ttgagttagc aaataaagtt ttagaagttg aaaatgaagt
     3541 aaattattta gaaaaaactt ttagaaattc acatatgatt agactaaata atggaaattg
     3601 tacaattgat gctggtgtac tttatcttga cttacttact aatctagaaa gaatttctga
     3661 ccactcaaca aatatagtaa aacaagtatt aaaacttaaa caaaaaatat agtgattatt
     3721 tttatatata atcttaatca atgagaagta agaagctata gataatgctt atatcattta
     3781 tggcttctta ttttttatgt aatactcaaa tttttatagt attctcttat tattaaacag
     3841 cttctgccta gaatattcca tatatatatg acttctttca taaaacttga actgcccata
     3901 actaaagtca caagtataca taactatttt ttaatcaatt ataaccaata tttattttat
     3961 ttatcttcat tttaattctt cattcaataa tgtaataaga ttatttttta tttggatact
     4021 attatatcat ctaaatatgt tattatatat ttggatattt tcatattaat aggagggatt
     4081 ctacaatgat aaattatatg gtagaaatta tatatagcga aaaaataagc gaagtagaaa
     4141 acatgttgaa aattgaaaat atacaatatg aatttacttc tcgtgatggc tttatatttt
     4201 ttggttttta tgagatgaca gatgcatata gatttgagac tttatgttca gatgaaaaaa
     4261 ttcgatttaa aagaatataa taaataaaaa aagtacatat ttctaaacta tttgtttttc
     4321 aaatttaggt tgtgttttaa tgtagtgttg atatcataaa caattttaat aataaaaaag
     4381 taggaatatt atattaatat gctcctactt tttactcata tttattaata ttatttatca
     4441 aaactaccct acaaactg
//
LOCUS       DAMKPG010000174         4387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMKPG010000174 DAMKPG010000000
VERSION     DAMKPG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.180"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            147..1622
                     /locus_tag="PC621_003567"
     CDS             147..1622
                     /locus_tag="PC621_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF4110677.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1709..2122
                     /gene="speD"
                     /locus_tag="PC621_003568"
     CDS             1709..2122
                     /gene="speD"
                     /locus_tag="PC621_003568"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF4110678.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2152..3003
                     /gene="speE"
                     /locus_tag="PC621_003569"
     CDS             2152..3003
                     /gene="speE"
                     /locus_tag="PC621_003569"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF4110679.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2993..3871
                     /gene="speB"
                     /locus_tag="PC621_003570"
     CDS             2993..3871
                     /gene="speB"
                     /locus_tag="PC621_003570"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF4110680.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4051..4251)
                     /locus_tag="PC621_003571"
     CDS             complement(4051..4251)
                     /locus_tag="PC621_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF4110681.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1732 a          497 c          752 g         1406 t
ORIGIN      
        1 gtaacttcaa aaaaacactt gtaaaaatgc tggaatgtta atataataaa tatcatgagt
       61 ttaataaaag taaaaataaa aaatagaaat aataaataat aaatattaaa aatatttgaa
      121 tatagagagg agatgaattt gaaatcatgg atagcgagtt tgcaaatgca gttgaattaa
      181 gctatgagtg tgctccatta ttagagtcgt taaaggaata ctcagagaaa gatatagctt
      241 gttttgatgt tcctggacat gtaaaaaata gaggtgtagc tatattaaat aaatatctag
      301 gtgaaagtct catgaaaatg gatataaatt catcaccaac tatggataat gtatctgcgc
      361 caaatgggat tataaaaaat gcacaagatt tgctagcaca agcatatatg gctgatgagg
      421 cattctttat aacgaatggc acaactcaag caatacatgc aatgattttg agtgttataa
      481 aaccaggtga aaaagtatta cttccaagaa atatacataa atcagtaata aatgcgttaa
      541 tactttgtgg aggaatacca atatttatac aaccagaatt tgatgaaaaa ctaggtataa
      601 gtttaaatat tactcttgaa aaagttagaa cagagataga aaaagattgt aatataaaag
      661 cattattttt ccttaatcca acttactatg gagtttgtgc agaccttgag agtattatag
      721 aactatgcca taaaaataat gttttagtgt tagttgatga agctcatggt gcacattttc
      781 catttcattt agatttacct ccttctgcaa taagtttagg agctgacatg gtagctgtaa
      841 gtatacataa aactggagga gcattgacac aatcttcagc actcttattg aatagagata
      901 atgtaagttt tgaaaaggtt cttcaatcaa tcaatatgct acaatcaaca tcagcatctt
      961 atttgcttat ggcaagtata gatggtgcta gagttaatct agttgagaat ggtgaaaagc
     1021 aactgtcaaa agcattaaat ctatctaggt atgcaaaatc taaattgaac aaaattgatg
     1081 gaataaaagt tttatctaca gaaatattga aacaaaaagg tgttaaattt atagatgaaa
     1141 caaaattgtg tataaatgta aaagaattaa atttgactgg atttgaagta tacgatttat
     1201 tatataaaaa tttttcaatt caagtagaat tgggagattc atataatata ctggcacttg
     1261 tgtccattgg aacaaataaa tcagatatag atagattagt aaaagcactt tctataattg
     1321 caaagattta tagaaaagaa tcaacattaa atgaatttaa tatggttcaa ataaatccaa
     1381 ttattaaact taatccaaga gaagcttttt atgcaccaaa ggaaagtgta gaaatcaatt
     1441 catgtataga tagaatttgt ggagaatcaa taatggcata tccaccaggt ataccgatta
     1501 ttgctccagg agaattgata acagaagaaa ttatggagta tattattttc ttaaaaaata
     1561 gcaatgcgta tcttacagat gtacaagata aaaacttaga tagaatacta gtaataaaat
     1621 aaattacttt attttgcaag ggcaagtgtt tgttaagtag agtttatcaa atagaactta
     1681 ttaaaagtat aaaggagttg gggttgaaat gaaatttgaa acactaggaa gacatatact
     1741 agtagaatac tataattgtg atgaaaacgt acttaaagac ccacgtctta tagaaaattt
     1801 tatgaatgat tcagcattaa atgcaaaagc gacaattgtg gattctgtat tccaccattt
     1861 taatccttgg ggggtttcag gagctgttat aattgctgaa tctcatttga caatacatac
     1921 atggcctgaa tatggttatg cagcagcaga tttttttact tgtggtgata tagacccatg
     1981 gaaaagtttt gaattattag agcaactttt aaagtctgaa agaagcgagt ctacagaaat
     2041 tccaagaggc ttaactacaa aaattaaaaa atattcaaaa aaagatttag gtaagattac
     2101 tcataaacca gaagcagtat aaaacataca agagaaagtg aggattatta tatggaactt
     2161 tggtatacag aagaatggac agaaaatgta agattttcta taaaagtaaa taaacatcta
     2221 tttgaaggaa agtctcaatt tcaaaggata gatgtatttg atagtgatga atttggaaaa
     2281 tttctaacaa tagatggatt aatgatggta acatataaag atgaatttat ataccatgag
     2341 atgataactc atgttcctat ggctacaaat ttaaacataa agaaagtatt agtcataggt
     2401 ggaggtgatg gaggtacagt aagagaatta agccgttatc ctcaaataga aaagattgac
     2461 atggttgaaa tagataaaat ggttgtagat gtatctaagg agtatatgga tatatgctca
     2521 tgtaaattag atgataaaag agtgagttta tattttgaag atggtgttaa ttttgttaag
     2581 tgtgcacatg ataaatcata tgacctgata atagtagatt caactgaccc aataggacca
     2641 ggcgaagggc ttttctcaac tgatttttat aaggattgtt atagaatact gactgatgat
     2701 ggaattttag taaatcaaag tgaaagtccg tattttgact ttaatgctaa agagatgaaa
     2761 agagctaata aaaaattaaa acagatattt ccaatatcag aagtttatca agcacatata
     2821 ccaacatatc catctggtca ctggttgttt ggatttgctt caaaaaaact taatccagta
     2881 aaaaatcaag atagaaatgg atgggagaaa ttaagtttaa aaactaaata ttataatagt
     2941 gatatacatt taggttcgtt tatgttacct caatacgtga aggagatgct agatgaagaa
     3001 taatttctat catatgaaca cttttatgag tatggacaaa aattatgaag aatctaatct
     3061 tatagtattt ggtgttggat ttgatggaac tacttctaac agacctgggg ctagatttgc
     3121 aagtagctct atgagaaaag aattttacgg tcttgagaca tatagtcctt ttttagattt
     3181 ggacttagag gattataata tatgtgatta tggagattta gaaattagtg ttggaagtac
     3241 agaacaagtc ttaaaagaaa tctatcaaga gacatataag attgttagag attcaaaggt
     3301 accctttatg attggaggag agcatttagt tacattacca gcctttaaag cagtacatga
     3361 aaagtacaat gatatatatg taattcattt tgatgcccat actgatttga gggaagaata
     3421 taataatagt aaaaattctc atgcaacagt aattaaaaga atatgggata ttgtaggtga
     3481 taataaaata tttcaatttg gtataaggtc tgggacaaaa gaagaattta aatttgctac
     3541 agaagaaaaa cacacataca tggaaatagg aggaatagat acgtttgaaa atatagttaa
     3601 tatgctaaat ggaaagaata tttatctaac tatagattta gatgtattgg atgcatctgt
     3661 gtttccggga acaggtacac cagaacctgg tggtgtaaat tatagagagt ttcaagagat
     3721 ttttaagatt ataaaaaact ctaatataaa tatagttggt tgtgatattg tagagttaag
     3781 ccctgattac gatacaacag gtgtatcgac agttatagct tgtaaaatct taagagagtt
     3841 atgcttaata atatctgata aaattaaata gagtatttta tataaatagt atatattata
     3901 atataagaag ttattttaaa aaagttagtt taagtttgac gcttttctaa agtaacttct
     3961 tatttttgta attaaaaata aatataataa acaacaaaaa agagccctcg atagctcctt
     4021 ttctaaaata tatatattta aattaaataa ttatattcta tttacgtttt cagcttgagg
     4081 tcctctatta ccttgagtta tatcaaagct aaccttttcg ccttcttcta aagttttgta
     4141 tccatcattt tgtatagctg agaaatgtac gaaaacatca tcttctccct ctacagatat
     4201 gaacccaaat cctttttcat tgttaaacca ttttactatt ccgtttttca ttataagaaa
     4261 acctccgcat tattaaaaaa tacaaactaa aaaaataagt ctgttctatt aatataacat
     4321 aaattttaaa taaaagcaaa taaaattttt aaaaaaatta attactttta gaatttaata
     4381 aaaattg
//
LOCUS       DAMKPG010000175         4299 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMKPG010000175 DAMKPG010000000
VERSION     DAMKPG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.181"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            246..584
                     /locus_tag="PC621_003572"
     CDS             246..584
                     /locus_tag="PC621_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110682.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            606..923
                     /locus_tag="PC621_003573"
     CDS             606..923
                     /locus_tag="PC621_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110683.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1073..1750
                     /locus_tag="PC621_003574"
     CDS             1073..1750
                     /locus_tag="PC621_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110684.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2078..2845)
                     /locus_tag="PC621_003575"
     CDS             complement(2078..2845)
                     /locus_tag="PC621_003575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF4110685.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            3651..4283
                     /locus_tag="PC621_003576"
     CDS             3651..4283
                     /locus_tag="PC621_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF4110686.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1736 a          533 c          531 g         1499 t
ORIGIN      
        1 ctataattaa catattatta attcgtttta ttattgtagt atggctatga aattatgaaa
       61 atatttctct aattaaaaat atgatttatc catatttgtt tattgtagat acagactata
      121 caaaaataaa tagcacttat ttcagtataa aaacatatat agtctatatt tagactaaaa
      181 aagtataaat agtctatata aaacaactta aactatgaat ataatctaaa gtggagggat
      241 aataattggc aaatatagga aaaataatag gagatagaat aaaagtctat agaacaaaat
      301 tgggatatag tcaagagttt ctagcagaaa aggcaggttt acatccaaca tatattggtc
      361 agattgaaag agctgagaca aatacatcta tcaatattat aatgaaaatt gctactgctt
      421 tagacatacc acttgaattg ttatttgcaa atattattac aacagaaggc gttgataact
      481 ctgtaccttt agaatgttat gaaatgatta ataaattaac tgaaaaagaa caaattgcaa
      541 tgctagagtt aataaagtgc atcattaaat acaaagaaat ataagtgagg gaaatagtaa
      601 caataatgaa ttatataggt aaaagactta aagaagaacg aaaaaaagca aatttaactt
      661 caaaagaatt tgcaaatatg gttggagtat cgccttggta tataactcaa atcgaaagtg
      721 gtaaaaagaa tccaagtctt aaaacattca taaaatttgt aaatatatta aatatttctg
      781 cagatgttct tataaaagat attacttcca ctggaaaaac atatttagaa aatgatataa
      841 atgaagagtt aaaagactta aattcaagag aactaaatct gatatcacag attatagaca
      901 tcataaaaaa gaataaaata taattcttta tattttatat aaaaaaaaag gttatttgaa
      961 ataaataacc tttttttgtc tttttgtgtt aacattgtct tatagtgcta atgagtacta
     1021 ttagaccatg ttctatggcc ttattttaat ataaaacgat tggagatagg ctatgattgt
     1081 gattgaaggt agtgataaat ttaagattgc aaaagaatat attgatgtag aatatactct
     1141 ttttagcaaa gtaactttta ggtatgaaaa gttgaaattt aaagataatg ctgaattgga
     1201 aaaaattaag atgtttaaat ataaaaatgg ctacatccct aataaattaa acctttcttt
     1261 tggatatgga ttctcttctt ataaaaagca aataattaga gaaactgtag atactttaag
     1321 attgacagaa attttttcaa gcgagaacat agaagatatt aaatttataa aagatggtac
     1381 aaaaaaatta gaaattagca tagagaaagt tgtgaaattt aaacgtcgaa aaaaaagaaa
     1441 ttatgtttgt tgctattgcc ctgatatgta tagagatata aaactcgaca aagaatctat
     1501 caataagata tacaacagaa aaataaaaat agaaagagaa gttaatattt ttgaagatga
     1561 ggatgttata ataaacaaga aagtattgaa gtttccaaag tcttggacaa agaatatgca
     1621 aaaatattgg ttaagtgaaa ataagtatcc catacattct actgtaattg atgatgatag
     1681 atataaatgt tgtaatgtac aatatacaaa aaatagagtg ataatattat attacatata
     1741 taaccattaa tatacataat tacaaaaaaa taatgacaaa tatacataat acaaatatat
     1801 cttctacttt agataaaaca gttattatga aaacttacta tctataaata atagaaatgc
     1861 tagtatacca tctataatct aatgtctagt aggttttaat ggcatatttt aaatatgcca
     1921 tagttgattt ataagatttt ctatatcact ttcaaaatac acactaaata gttgcttttc
     1981 agatataatt gcccaattta aattttacta tctgaaaagt tttttatatt taatttatat
     2041 cttcaaaaat accgtctaat atttctatat aattaattta attaatactc ttatgtaata
     2101 gattaagtac tgaatttaac tctttaaatg atatttgccc tggtgctgaa acactttttg
     2161 cagctccaaa agtcaatgca gaaccaaata tttcaccaca caatctactt ataacaccca
     2221 ttcctgacat tgacattgtt attattggtc tatctgcata aattttaaac atttcatttg
     2281 tagcttcaag tagcactaac acatctcttt cattttgcgg cataacagct actttgggta
     2341 aatctgcgcc caattcctgc attctacata aacgagatac aatctcttct ttttttggag
     2401 ttttattaaa atcatgatta gatataatta ctttcacttc ttttttatga gcaaaattaa
     2461 ctacttcatc tatgacctca tctcccataa ataactctac atctattaaa tctactaaac
     2521 ctgtattaga aatttcttta ttcaaagtag tataataatc tcttgatatt aatttttctc
     2581 ccccttctac tacacttcta aatgtgaata ataaagggat atcatgtata taacttctta
     2641 attcatagag aacttcctta acttctttaa tattttctac attctcaaaa aaatcaactc
     2701 gccactctat aatatccaaa catgcatctt ttaactcttt agcctctttt ataatatctt
     2761 ttttattttt cccaataatt ggtacacaaa ttttaggtct accttctcca atagtaatat
     2821 tttttacttg aacttttctt ttcatattct cctccataat cagaaattct ataggacttt
     2881 aaattctatt ttctgcataa tttaattata cattaaaaat actatacatt ataaaaatac
     2941 taatttaaaa ataattttta ttctaattct taaaacaatc ccacatattt tagaaaaggt
     3001 actctataat atacaaacac ttctttttta cctaaattta ttttaacatt ctataatttt
     3061 cacaatattc tttaaatttt actcatataa acaatattat tctggatgct aatagaatta
     3121 atctataaat catagtataa ttatgcagaa ataactcgaa tttcactact tataaagaga
     3181 ttgaatatta atgaatctaa atcatttgca ttacttcaga gttttgacta aagttgaaca
     3241 ccatacaaaa gtagctactc aattgtttat accccaacca agcttaagta atgttatata
     3301 gattcttgaa aaagaatttg gtatcttttt atttgaaaaa caaggtagaa atgtttccct
     3361 cactaaatac ggaagttttt ttctaaccta tgttgataaa gcattaggtt aattagaaaa
     3421 agtttacata taaaatatat taaattgttg caagttttat tgttttgata tacaatcaac
     3481 taaacatata atctttataa aatactatac agttaaaagt ctgttttaaa aaaaaatgta
     3541 taaattataa atcaaaatat agatactatc aaggagtgat tctgtgaaga gatgtattgt
     3601 agtagtggaa tagtcaaaaa cataatatat attaaaggag agatttaaat atgactattc
     3661 caaattcaaa taaggtatat ccaagaagta atgattatca aactgtatat cttaaaaatg
     3721 taataacaag ggaaaatata gaagttggag attacacaat atataatgac ttttataatg
     3781 acccaagaga ttttgagaaa aacaatgtat tatatcatta ccctataaac aatgataaat
     3841 taataattgg taagttttgc tcaatagcat gtaatgcaaa atttctaatg actagtggaa
     3901 atcactcaat gaaatcaaaa tccacatata cttttcccat cttttatgaa gaatggaaac
     3961 tcgatatcaa ccatataacg gacgcttggg acaataaggg taacattata attggaaatg
     4021 atgtgtggat aggttatgat tctataatta tgtcaggtgt aaatattggt gatggtgcaa
     4081 ttattggaac aagagcagta gttactaatg acgtctcacc gtattctatt gttggaggag
     4141 tacctgcaaa aattataaaa aagagattta ataatgatac aatttcaaaa ttattagaaa
     4201 ttaaatggtg ggattggcca tataaaaaaa ttcaatcaaa cattaagcat attcaatcag
     4261 gaaatattga taaattagtt taatatttta taatttatt
//
LOCUS       DAMKPG010000176         4168 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMKPG010000176 DAMKPG010000000
VERSION     DAMKPG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.182"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(293..1153)
                     /locus_tag="PC621_003577"
     CDS             complement(293..1153)
                     /locus_tag="PC621_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF4110687.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            1302..2192
                     /locus_tag="PC621_003578"
     CDS             1302..2192
                     /locus_tag="PC621_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4110688.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            2410..4164
                     /locus_tag="PC621_003579"
     CDS             2410..4164
                     /locus_tag="PC621_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF4110689.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1699 a          437 c          652 g         1380 t
ORIGIN      
        1 tgatttaaat tattttgtag ttttataata tttaaatttg tatttgattg ttataaagta
       61 aagtaagtaa taaagttaga ctcaataatt ctaaaatttc taagcacact atctaaatga
      121 gaacgtattt ggaaatactt gtataaaaca ataattgata catttaattt ttatcttaaa
      181 aataacaatt ataatatata aatagaaagt ataatatata aatagcaagt ataatataac
      241 atataaaaag ttgaatttgt atatgttaat tatacttgct atttattgtt agttattcta
      301 tttttttaaa tccattacct aaagcttcat gtatatctgt aattataata aaagcattag
      361 ggtcgacatt ttttactaat ttttttaatt cgacaacttc tttcttttca actactacta
      421 aaagtacatt tttattttct ccagtatatg caccttctcc ttttagaata gttgcacctc
      481 tatctaaatc ttccattatt tttttactta tatcttttga aaggtcagat ataatagtga
      541 atgatttgga ataattaaat ccttcaataa gcaagtcact aacttttact aaaatgtata
      601 aagccacagt tgaataaagt gcaatttcaa cctgtctatt tactatacct gataaaacaa
      661 ctacaacgca gtctacaata cccattaaaa caggtatact aaatgtagga aaatatttat
      721 ttagtaggag tgccattaaa tcagttccac ctgtagagcc atttattcta aaaataagtc
      781 cttgaccaac tcccattaaa atagaaccag atataattgc aagatacata tcatttgtta
      841 tatctaatgt agccatattt gcagtaagct taagtgctat ggtaagaaaa attattccta
      901 aaaccgtttt aaaacaatct ttttttgaaa gtattttgta agcaaatatg aataatggaa
      961 catttaaaag taaattgaca atccaaagtt ctacaccgaa taatgaattt aaaacaactc
     1021 caagtcctgt tataccacca gcagcgattg catgtggatt aaaaaataaa ttaagagaaa
     1081 tggccattaa tatacagcct acaattaata ttaaaatttc tgaaaaagtt attctatctt
     1141 ttttcaataa catcactcct tatatataga ttataatata tattatatag tttttccaat
     1201 ttttaataaa agaaataaaa atatattata aaaacaattt gattggaatt ttcagaaaat
     1261 taatgctata attaaaagta aatttaaagg gaggttataa aatggctaaa ttaactctag
     1321 aacatataaa aggaaataca tattatattc cactccctac aatagtaggt gtatacgtag
     1381 atggtaaaga tgcaatttta attgacagtg gaaataataa ggatacagct agacaagttt
     1441 taagattatt agaagaacat aatttaagtc ctaaattaat aataaacact cattcaaatg
     1501 cagaccatat aggaggaaat gcatatttga agaatcaaac aaaatgcaag attgctacga
     1561 ctaaaattga aggatatttt acagaaaatc caatattaga atcagcattt ttatatggtg
     1621 gctatccaag taaagcactg aagaataaat ttttattagc aaaggaaagt gaagttgatt
     1681 atattatacc ttcaaatggc aaaattgtag atacagaact tgaagcgatt tctcttccag
     1741 gtcattattt tgaaatgatt ggtgttagaa ctccagataa tgttttgttt gttgcagata
     1801 gtcttacacc agagaatata attactaaat atcatttttt cttcctactg gatatagatt
     1861 cacagtttaa gacactagaa aaattgagaa tgttagaggc tgatttcttt gttccaagtc
     1921 actctgtcaa aactacagat ataaaaagtt tgattaatat taataagaag aagatggaag
     1981 aaataataga taatattaaa aaggtttgtt atgagccagt tatgattgat gaagttattg
     2041 aaaaaatgtg tgatttgtac aatgtcaagc ttgatgctaa tcaatatgtg ttggttggaa
     2101 gtacaatacg ttcttatata acttatttat atgagaataa tatggttgaa tatatatttg
     2161 atggtggtaa gatgatgata aaagctgtgt aaatagtaat aattttcaaa ttatatgatg
     2221 aaattgtttt agtatgactg ttatttgaaa agtaacataa aaaaatttat ataaataagc
     2281 taagatgatt gatataaagt tactgataat tttaaggtaa tatatacgtt actataatat
     2341 tatgttatta tttgtaaata aggggataat ttataagcaa attaaagacc ttaatttaca
     2401 ggaggaatag tgtatattat gaataaaaaa ttcaaaaaat tcagtaaaaa actaagtata
     2461 ttaattttag ttttgactat gtttcttttt aataatttaa ctgcaaatgc acagattaaa
     2521 gaagattcaa taataggaat tgatagatat gaaacagcag gacttatagc agatagacaa
     2581 acttatgata ctgtaatatt agttaatgga gataaaaatt tatctgatgg gctttcatca
     2641 agtggattag ctggagctat taacgcacca atacttttga ctaaaaaaag tgaaatacca
     2701 aaagctactt taaatagact agataataag agtttaaatg ttgtaaaaaa agtatatata
     2761 attggtggtt ataatactat tgaaaattca gtagaaaaag atataaaaag aaaaggtatt
     2821 caagtggaaa gaataaatgg taacaataga attgaaacta gttataatgt agctaaaaaa
     2881 ataaatgaag taagtagtgt aaaagaggta atgcttacaa atggatttgt aggagaggct
     2941 gatgctatga gtatagcacc tgtagttgca aaaaataaag gagctataat acttacagat
     3001 gggaaatcag ttccatttaa gactgaaggt ttaaatgttt acgctatagg tggcaaatct
     3061 gcgataaatg aggatttagt aaatgaaact aaagcaacta gaatcggagg aaatgataga
     3121 tttgagacca ataaaaaggt aatggaaaag ttttataatg gagcgacaga tttttatata
     3181 acaaatggat atcaattagt agatgcacta acattatctc ctttagccaa agaaaaacca
     3241 atcgttttag ttgctgatgg aagtaataaa ggtattttaa aaggagctaa aagtataaca
     3301 aaagttggag gtattgatgc caatatatat aaacagtgtt tagatatgat taaatataat
     3361 aatatggatg tgactccaaa tattttaaaa cacttaactt cagttgaagg aaatgaagtt
     3421 tctgaaatac ttatagaatc agataattta ggtgtggttg ttgaaaagac gaataccaat
     3481 aaatttgagt ttgattacgt aagtgtaact aatgaaaaaa attgtacttt ttctgtatat
     3541 aaagaaaatt cttctaataa tgtaaaaaat ggaaagctag ttgtaagtgc taagaaaaaa
     3601 atagataaga aagatagtga tttgcaagat atgaatggcg acaatatgat aaatgcaaat
     3661 aaagatagga tggtaaatgt gataaaaatt ggagttcccg ataaaatgta ttctaatttt
     3721 aaagtagatg ttgaaagtgg ttcagtagaa ctttataata ttaaaggagg agctactgtt
     3781 gatgtcaatg acggaattgc taacattgtt gataacaatg tgacaaatcc tttcaatata
     3841 aatactaata gtgggatttc aggtgtaaca gctgaaacta tatcatctga gataaaattt
     3901 agagcaaaca atggaattgt aagtgtaaca gctacaaata taaatggcaa tataagttta
     3961 tttggaagcg ataataaagg tacctttgat ggaatattta aattaaatct gaaaaaagaa
     4021 ccatcaaatt tacacttgaa gttagttgga aatgggataa ataaattgcc agaaggttgg
     4081 tctaaagatt atattttagg aaatggacaa ccaattattg aagttaaaaa taatggcata
     4141 aataatatat caattggaga ataaaaac
//
LOCUS       DAMKPG010000177         4117 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMKPG010000177 DAMKPG010000000
VERSION     DAMKPG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.183"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            171..2270
                     /gene="nrdE"
                     /locus_tag="PC621_003580"
     CDS             171..2270
                     /gene="nrdE"
                     /locus_tag="PC621_003580"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF4110690.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2283..3254
                     /gene="nrdF"
                     /locus_tag="PC621_003581"
     CDS             2283..3254
                     /gene="nrdF"
                     /locus_tag="PC621_003581"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF4110691.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3330..3956
                     /locus_tag="PC621_003582"
     CDS             3330..3956
                     /locus_tag="PC621_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF4110692.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1646 a          447 c          766 g         1258 t
ORIGIN      
        1 ataatagata aagcactgac tatatgtagt gcttttgttt ttgaaaaata cttattgcat
       61 ttctataatt atagattaat tttgggattt caactgataa gtaccaaaag aaataataag
      121 caaattaagt gaaattatgg cacaagatta tgttaaaagg acggtaaata atgaattgga
      181 tagagcttaa caatcaagtg ataattaaaa atgaacaagg taagtaccaa ttggaaaagg
      241 ataaggaagc cttaagtagt tacatagaag aatttataca accaaagttg agaaaattta
      301 ataacctaga agaaagattg aagtatttaa tagatgaagg ttattattta aaagaagtaa
      361 taaatcagta tagcatagat gttattaaaa atatatatga tatgattgat aaacataagt
      421 ttgaatttca atcatatatg agtgcaaata agttttatca aaattatgca ttaaagagca
      481 atgatggaaa ggaaatatta gaaacatata atgataaagt gttaattgta gctttgacac
      541 taggaaatgg cgataaagat ttagctttaa acttggcaga taaaatagta aaacaagaat
      601 ttcaaccagc tacacctact tttttaaatg ctggaagaaa gagggctgga gaaatggtat
      661 catgtttttt actatctgta gaagatagta cagaaggtat atcatatgct atatcttcat
      721 caaatcactt atcaaagatt ggtggaggag ttgcactaaa tttatctaaa cttagagcat
      781 ctggcgaatc tatcaaagat atagaaggtg cagctggagg tgttgttgga gtagctaaaa
      841 tgttagaaca atcatttagt tactttaatc aaatgggagc tagacaaggt tctggtgcgg
      901 tgtaccttac tgtattccat cctgattttg aattgttgat ggatactaaa aagataaatg
      961 cagatgaaaa aataagactt gctacattat cattaggagc tataatacca gataaattta
     1021 tggagttggc agaaaaaaat gaagtagcat atgcatttta tcctcatact gtgtataaga
     1081 aatatggagt ttctcttgat gagatagaaa tggatgagtg gtatgataaa ttggttaatg
     1141 atagtgatat aagaaaaaaa gaaataaatc ctaggcagat gttgacaaaa atagcacaaa
     1201 tgcaacaaga aagtggatat ccatatgtag tgtacataga tacagctaat agagaacata
     1261 cattaaaaga tgttggaatt attaaaatgt ctaacttatg ttgtgagata tttcaatatc
     1321 aaacaccttc agaaatagag gggtatggag gaaaaaatga atggggacaa gatataagtt
     1381 gtaacctagg ttctttaaac atagccaatg taatggataa taaaactata gaaagtacag
     1441 ttgaaacagc aataagagca ctgtcatttg tagcagatag cacagatata aaaccagtgc
     1501 caacggtaag caatagtaac agcaagtctc attctatagg attaggagct atgaatttac
     1561 atggatactt agtaagagaa aatattttat atacttcaga tgatgctata gatttttcta
     1621 atgtattttt tgcaatggtt agatactatt caattaaagc ttctatgaaa atagccatag
     1681 aaagaaatca aacttttgaa ggatttgata aatcagaata tgttaaggga agaaatagca
     1741 aggttttatc aaaatactat gaacaatctt atctacctaa gtcagaaaag gtaagagcat
     1801 tatttgaagg gatttatata ccaacaaaag aagattggac aaaattattg gatgaagtaa
     1861 aagaaaaagg gatatataat gcatatttaa tggcagtagc accaactcaa agcattagtt
     1921 atgttcaaaa tgctacatca agtataatgc caattactga gccagtagaa gtaagaacat
     1981 atggagactc tactacaata tatccaatgc catttttaac aaatgataat atgttgtatt
     2041 atcaatcagc atacaggatg gatatgagaa aggtcataga tttagtggca acagttcaaa
     2101 accatgttga ccaaggtata tcaacaacac tgtttgttac agatgaaaag acaactagag
     2161 atatagctag acattacata tatgcctata aaaaaggttt gaaaagttta tattacacaa
     2221 gaacaaagat gacaagagat actcatgagt gtctagtgtg ttcagtatag ggagagaaat
     2281 atatgacatt taatttacat aaaatacata atgcagttaa ctggaataga gaagaagatg
     2341 gatttacaca agctttttgg gaacaaaatg taaaacaatt ttggcttcct gaagaaatat
     2401 ctgtttctaa agacataaag gtatggaatg aactaagtaa taaagaaaag gaattatata
     2461 aaaaagtatt gggtggttta acattactgg atacaaaaca ggggaataat ggaataccat
     2521 ctatgatgag tttaacagaa aacctacaaa gaaaagctgt cttgtctttt atggggacta
     2581 tggaagaaat acatgcaaaa agttattctt ctatatttat gactctactt tctaatttag
     2641 aaatagatga attatttgaa tggatagaaa cagaaccaac attacaaaga aaggctgatt
     2701 tagttttagc tcaatacgaa aatactacaa atcaagaggg attataccta tcaatggtta
     2761 caagtgtatt tttagagagt ttcttgtttt attcaggatt cttttatcca ttgtatctat
     2821 ctggtcaagg aaagatggta gctagtggag aaataatatc gcttattctt agagatgagt
     2881 ctttacatgg taagtatata ggattgctag cacaagaaat atacgattca tttgataaaa
     2941 tggataagaa gatgttagaa gagaagatgt attctatact ttatagcctt atggagaatg
     3001 agatagagta tacaaatgtt atatacagag aaagtgggct tgaaaaagag gttgtgaact
     3061 ttttaaaata caatgcgaat agagctttag aaaatctagg gtttgagaag ctatatactg
     3121 tagatgctat aaatccaatt gtattaaatg gtcttagcac tgaaaccaaa acacatgatt
     3181 tcttttcaac taagggtaat ggataccaaa aaggagtgta tgaggaatta gaggatgaag
     3241 attttataat ataatttata attatgacac actgttgtca cttttagcgt gataatatca
     3301 atgtataaaa tatatatgga ggtattttta tgaaatatga atggagaaaa agagataaag
     3361 agttatattt acctaaaaag aatcctgtaa taattgaaat accagaacag aaatttatca
     3421 tgttaaaagg gagtggagac ccaaattcta aagaatttat tgaagcaata ggagtacttt
     3481 attctttggc atatgcaata aaaaccatgc ctaaaaaagg aattgtacca gaagggtatt
     3541 ttgactatac ggtttttcct ttagaaggta tatgggataa gggagaaaaa agtaaaggct
     3601 tagatgtact tattaaagaa gatttaattt acactataat gatacgtcag ccagattttg
     3661 ttacacaaga cctgttggat aaagctactg atatagtaaa gaagaagaaa cctcataatc
     3721 ttcttgaaaa agttaggttt gggtctatta atgaagggct atgtgttcaa atgcttcata
     3781 taggttcata tgataatgaa ccagaaagtt ttgacattat gaacgaattt tgcaaaaaaa
     3841 ataaacttat aagaaaatct tacacacatc gtgaaattta tataagtgat gctagaaaag
     3901 tatcgcctga atcattgaag acggttttac gttttgaagt agaaagggaa gtataaatat
     3961 tttgaataat ttataagagt tataaaaagt gaagtctgtt gagacttcat tttttgatgc
     4021 aattttaaaa ttaaatttga tgtttcataa tattttaaaa actttctact tttattagtg
     4081 tgaataactg ttgctattaa ataataatac taattat
//
LOCUS       DAMKPG010000178         4086 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMKPG010000178 DAMKPG010000000
VERSION     DAMKPG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.184"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            164..1567
                     /locus_tag="PC621_003583"
     CDS             164..1567
                     /locus_tag="PC621_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4110693.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1658..1898
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1941..2639
                     /locus_tag="PC621_003584"
     CDS             1941..2639
                     /locus_tag="PC621_003584"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF4110694.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2662..3663
                     /gene="trpS"
                     /locus_tag="PC621_003585"
     CDS             2662..3663
                     /gene="trpS"
                     /locus_tag="PC621_003585"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF4110695.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
BASE COUNT         1530 a          454 c          739 g         1363 t
ORIGIN      
        1 atttttaata agatttgatt tcaaagtatt taaggtttat tattaaaact gtttttgaat
       61 atgcaaatat tttgaaaaat atcagtttaa taataatgaa tctattttat taattttgaa
      121 attaaattta aaataattaa tagagagggg aaatgaaatt aggatggaaa agaaaaaaat
      181 aggcctatgg agtttggtat ttatgaatgt atcagcttta tatggtataa gatggatagc
      241 taagtctaca gcatcaagct ttggtttagg attaggtgca atacctatgt ggttattatt
      301 ctcatttata tactttgtac caggagcttt aatttgcgct gaattagctt ctacatatca
      361 aaagggtgat ggaggtcttt acgattgggt taaggaagcc tttggagaaa agtatggatt
      421 tttagtttct tggttaaact ggacagctaa aatattttgg tattcttcat ttttaacttt
      481 cttagcagta aatgtttctt atacaatagg aaatccagct cttgctgata ataagatgtt
      541 tgtacttata ttatctttaa taatattctg ggcattatct cttatagcaa ctaaaggaat
      601 aagttttaca aaaatattta ctaatacagg tgcattaggt tcaacaatac caagtgctat
      661 acttataata ttttcatttg tagcagtatt tatgttgaaa aaacatgata tagcttctac
      721 atatacaata gcttcaatga ctccaaaact taatatggat gcatttgttg ctatatcagc
      781 tataatgttt ggtttagcag gagcagagac tgctgctaac ttcataacag aaatagataa
      841 gcctgaaaag aacttcccta aggcaatatt aatatcagct ggaatagtag cttcattata
      901 tgtattaggt tcaattgcaa taacaatgat aataccacca gaccaaatta ctgcttctaa
      961 aggtgtatta gatgcacttt ctgctgtttg tacgtcttta ggaattggtt cttggttggt
     1021 tcaattaata gcttttggta tagctttctc agtattagga gcaatagttc tttatatagc
     1081 atcaccaata aaaatgttat ttggtagtgt taaaaaagga atatttccag atagtttggt
     1141 agaggtaaat gaacacaaga taccttctaa agcagttata cttcaagcaa taatagttac
     1201 tataatacta ttggtgacta cattaatgcc ttctgttgat gctatataca atgttcttgt
     1261 aactatgaca gcattaactg cattattccc atatgtatta ttatatgctt cttatattaa
     1321 actaagaaaa gagagaccag atgaaattag accttatact atggcaaaat caacaagtac
     1381 ttgcctaggt ttagcaaaaa tggttttagt tgtaactgta gttggtatat tattatctgc
     1441 agcaccagtt atgccgacat ttgctgaaaa tgttatatat gagatagaga tgattggtgg
     1501 aggtcttcta gttatattat ctggtctatg gttatggaat agatatgaaa agaaaacagc
     1561 taaataaaat gtattttaaa aatttctttt ggatattata atatatagtt gacaatttat
     1621 agtgtaagga atattataaa ataaagaaat taataaatta aatcctgaga taagaaatag
     1681 taaataaata tatttttgca cagagaatta aggatgctga gaacttaata aaaagtattt
     1741 attgaatggg tctttgagga gtaaaatgaa atgtaattta agattacaga gtatttgata
     1801 tcgtgtatgc acgttatagc atatattaag atatatatat tcagttttga gtgtatataa
     1861 atttaggtgg taccacggaa aatatatccg tcctattgat ttaggacgga ttttttaata
     1921 tagaaaattt aggaggaaaa atgaacgtaa taggaattat aggtgcaatg gatgaagaag
     1981 ttagtatttt agttgattta atggatatta gagagactat aaaaaaagct agcttagaat
     2041 tttataaggg aattttgaaa ggtaagaatg tagtgttagt aaagtgtggt ataggtaagg
     2101 ttaactctgc tctttgtgcc cagatactaa taagcgaatt taaggttgat gctattgtta
     2161 atacaggtgt tgcaggagct ttaaatgaaa aacttgatgt taatgatata gtaatatcaa
     2221 ctgatgctat acagtatgat gtagatacta cagcatttgg agaccctaag ggagttatac
     2281 caagaatgaa gacttctgta tttaaagcag atgaaagact tatagatgct gcttacaaat
     2341 cttctgtgga agaagtaaag actcataaag tattaaaagg aagagtagtt acaggagata
     2401 aatttataaa ttctaaagaa ttaaaagaag aattagtaaa tgattttggt ggatattgtg
     2461 gagaaatgga aggtggagct atagctcatg tatgctattt aaataatact ccattcgtaa
     2521 taataagagc aatgtctgat aaggcagatg gaagtgctga tgttacttat gatgtatttg
     2581 tacatgatgc tgccaataac tctaaagaca tagttttaaa tatgttaaaa tcaatataaa
     2641 tatataagga ggtacaaaaa aatgagtgaa aaaaaagtta tatttagtgg agcacagcca
     2701 tcaggcaaaa tgactttagg aaattattta ggagctataa aaaactggac tgaacttcaa
     2761 gataattatg attgctatta tagtatagtt gatttacatg caataacagt acctcaggac
     2821 cctaaagttt taagagcgaa tgctatagaa ttacttgctc aatatatagc ttgtggatta
     2881 gacccagaaa aaaatactat atttatacaa tcacatgtca aagagcatgt tgaacttatg
     2941 tgggtattga atactatgac ttatatgggt gaattaagta gaatgactca attcaaagat
     3001 aaatcacaaa agagtgaagc aaatctaaat gcaggtttat tcacatatcc agttcttatg
     3061 gccgctgaca tattgttgta tcaaactgac ctagttccag ttggagatga ccaaaaacaa
     3121 catttagaat tagctcgtga tttagcaaat aggtttaata atagattttc acctactttt
     3181 gtagttccag aaggatacta cccaaaaggt ggagcaagag tgatgagttt gcaagagcca
     3241 actaaaaaaa tgagtaaatc cgattcaaat gaaaatgcat ttatactatt agcagatgat
     3301 tcagattcta taaagagaaa gataaaaaga agtgttactg attctttagg tgttgtaaaa
     3361 tacaatgatg aacaaccagg tattaaaaat ctaattgata tatattctaa tttaagtaaa
     3421 aagagtgtag aagagattgt aaacatgtat gaaggaaagg gttatggcat atttaaagaa
     3481 gatgtggctg aagtagtatc agaagcttta agacctataa gagaaaaata tgttgacctt
     3541 cttaataata aagattattt agaaaaaata tatagtatgg gagcagaaaa agccgaaaaa
     3601 caagctagaa aaactcttag aaaagtttat aaaaaagttg gatttataga gagaagatac
     3661 taattattag ctatctttac ttgataaata aattttaaag ttattaaaaa ggtatatgat
     3721 atgaaagaga gagtctcaaa gtgaactttt gttcatgaga cactcttttt tgtatgaaat
     3781 aaaatatatt ttaatcactt caacaatgaa aaagaggatt atctctattt tgagacagca
     3841 tcttttgata ataaaaaaag atgagctttt gctcatctca aaggggttag attattctta
     3901 ctttggggaa agtagaataa aggtaaaagt taactgatgt caactttgtc ttttttgcat
     3961 atcactactg agcataaaaa aatcagaaaa ccatagcctt ctgaaacaac ttctaacata
     4021 cattgtattt tatacccaat atgtaattta tgagttaatt ttaactctta taatggaaat
     4081 tgtcaa
//
LOCUS       DAMKPG010000179         4073 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMKPG010000179 DAMKPG010000000
VERSION     DAMKPG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.185"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(204..587)
                     /locus_tag="PC621_003586"
                     /pseudo
     CDS             complement(204..587)
                     /locus_tag="PC621_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      814..911
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            966..1142
                     /locus_tag="PC621_003587"
     CDS             966..1142
                     /locus_tag="PC621_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110696.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            1368..1568
                     /locus_tag="PC621_003588"
     CDS             1368..1568
                     /locus_tag="PC621_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF4110697.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1732..2031)
                     /locus_tag="PC621_003589"
     CDS             complement(1732..2031)
                     /locus_tag="PC621_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110698.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            2253..2435
                     /locus_tag="PC621_003590"
     CDS             2253..2435
                     /locus_tag="PC621_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF4110699.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2506..3351
                     /locus_tag="PC621_003591"
     CDS             2506..3351
                     /locus_tag="PC621_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110700.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINNSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(3575..3739)
                     /locus_tag="PC621_003592"
     CDS             complement(3575..3739)
                     /locus_tag="PC621_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110701.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1544 a          506 c          471 g         1552 t
ORIGIN      
        1 atctatagtg ttacttatat tttatatata tggctatttt tcaacatttt acaatatata
       61 taacatttat ttttttaact tttgtcagta aaatgtcagc aagacacttt atccacaatt
      121 tttatataag ttttccacaa cttatcccca gtattatcca cataaaaaag gtagctctta
      181 tattattagc taccttttat ttattaatct tcatatttcc agctttttat ccaacttcct
      241 atttctatta ttttttcttc agttatttct tctccatcat gtaattttga aattaattct
      301 gataaaggat tgttatgtaa acctctaaaa actctttttc cctctatttt gagatattct
      361 ctctctttta ttaatactgt atagtgtaca tgcctcccaa ttctctatga tattaaaaaa
      421 ccttttttgg ttaatctagc taaaagtata attgtagttg tatgcttcca accatatgtt
      481 ttttctattt catctgcaac atcttttgat attacagttt tataaccagt attccatatg
      541 catttcataa tttttaattc tgtatctggt aattttttat ttagcataat atattccttc
      601 tttcaattta tgtcacttct tcaaatttat ctagcttaaa ttataatagg agttttttag
      661 aacaaaaact atagttaatt tttaccaggt actctacgtg tttagtctgt tttttaagtg
      721 taaaataatg ttcatatcta taaatttatt taactttcaa acaatcctgt tgaataataa
      781 ctaattctat tgtattattt tagttgtaga ataaaactaa atcggcaaaa ctagagaaat
      841 ttagtgacgc aaagctatag ggactaagac ttatataaat atcttatgag ttatgtcagc
      901 cagttgccaa agagatagtg ttctttttgt ttttatgaaa gttttttagg gggataataa
      961 tttatatgtt tggaaataaa atggataaat gtacacatgt attgactgct tatattagcg
     1021 gttcgtatga ttattgtaat tttttagata cacaattaga tgattttata ttagagtacg
     1081 gagaaagtgt agtagagtct tgtttgcacc aagtgatggt attggtaagt aagtataatt
     1141 agatagacat tgatttctag agaacccttt ttagttttat ctacatttat attaatatat
     1201 agcaatgtaa aacgcttaaa tatattttta atattaagaa taactaaaat gttgactaaa
     1261 aaatataatt aagtagacat ttctattaat ctatgataca atagtatttg tggtaataca
     1321 caattaaatc aagtatatct tgatataggt taataggagt atttttaatg aataatggaa
     1381 tagtgaaatg gtttaataat gaaaaaggat tcggttttat atcaatggaa ggtagagatg
     1441 atgtgtttgc tcatttctca gctatacaaa cttcaggatt taaatcatta gaagaaggtc
     1501 aacaagtaag ctttgatata gttaaaggtg ctagaggtcc tcaagcagaa aatataacta
     1561 tattataata tatagatttt tcgggaaaga ctctttttag agtctttttt tattgttagt
     1621 atatgtaaga taatcattgg tgaagtaaat aaggtatatt gagtaatgtt cttgatagtt
     1681 tatatgctaa tgaatttcaa aaaaataagc actcctataa aaagagtgct cttaatgtaa
     1741 atataagcct tcattggtcc agcctaatac cccgtattct ccaagttcta actctttttt
     1801 atatacatta ccactttcag ccatatctaa cacaatcatt tcttctttag aatcaatttg
     1861 taaaatatga ggactgtgtg ttgtgacaat tatttgtgtt tttaggagaa ttgcttttaa
     1921 tgtaataaaa agtttacata caacatagtt aatataaaac aaaattattt ttgagaatga
     1981 gctaaataaa atcttaactt tacataccac atagttgata taaaacccca aaataaactt
     2041 agcatctcca atgtttacac atatacaatg ctcttaaaat tgcagtgaac atgcaatagt
     2101 gcaattgata atacttatca tatattctca ataccttata tttcaactgc taaacctcaa
     2161 tttgtcacaa atatcgctca ctgcaaaatt tctatatttt tattatatca tagcatataa
     2221 ttaatatttt caattaattt ttacatttaa ttatgtttaa attctataat aatgcaactt
     2281 taatgtttct ttctggaact aatataaaaa ctatttcaga aagattaggt catgcagata
     2341 taaaaataac tatgaatagc tactctcagt ttttagaaga gatggacaga gaagcatttg
     2401 aaaatttgag caaaatacta tttattgtat tttaaagaag atgacttatt caatatttga
     2461 tgctagaata taattaataa aaatttaatt ataggtgatt gtattatgaa cattattaaa
     2521 agcataactt tactcttaac attattaatt gtgatattaa tgacaggatg ttccccaaga
     2581 gaaagtgcta gtgaagttgc aaaaaaatat attgataaga tgaactctgg tgactatgaa
     2641 acagcatata gtttattaag taaagatagt aaaaaaaaaa ttagtataga aaatcttgaa
     2701 gaatttcaaa atattttagt tagtacaaaa agaataatta gttataaaat aggtaaagaa
     2761 aaaatcataa aaaaatataa acatgatgga aaagaatata aaaatgttgt taaattagaa
     2821 gaaactttta atataaaaaa ccttctttat aaaaaagatg attctctaaa agtaaataga
     2881 tattttgtag ttgaaaataa caaatacaag ctattattgt atgaaagttt caaaaaagaa
     2941 tttggtatta atagtatgga ccttgctcaa cttaagctta atgattttgg actaaaatac
     3001 tttaaagaag ttgaccttat actaaaaaaa gctattgcta ttaatcctac tgattcaaag
     3061 ctttattatg cccaagcatg taattatatg aattttgatg gatttcctgg ttattcagat
     3121 acaacatata atgctttaga ttcaattaat aattctttaa agtatttaga taaaaatgat
     3181 tctgactaca aaaaaaatgc aagtaatata tataacttaa aaggagttat tcttatgttt
     3241 cacaaaagat ttgatgaagc acaacaatat ttaaataaag ccttatactt taacaaagat
     3301 aatgaagacc caaaatataa tctaaataca ataaaaaaac atctaaacta gatatttaat
     3361 ttttgaattt ataaaatatt tttttatgta taattcagca ctgaatcata atctctacta
     3421 aactaattac aagtttaaat tataaagact tatttctata taaatattac tctataacaa
     3481 taagtttctt atttaaattt taaaatatta tctaaaaatc ataaaaaaga aggtataaag
     3541 agctaccctc aagctcaata taccttataa atatctaaac ttcatttttt actaattttt
     3601 ctatatcttt gataatatgt tctttagatt tttttccagc tatggcatca aaaattgcaa
     3661 cagccttttt ctctccaaac ttttctgcta catcattaaa gtctaaaaat ctaacacttt
     3721 cttttggttt accaaacata taacacctct catacatttt tatatattta ttataacttc
     3781 tatagactta tttttcacca ataaacgtta gacaaattag tccctttatt tttttaaact
     3841 agctttacct tcaaaattct taacaaaaac agtttattct actctataaa taaaaataga
     3901 caatttaatt tgtaggaaac tgataataga atacagtaaa taaaactttg tttaatctat
     3961 ctgaaaaagt tatctattaa aactgctatg aatttataaa gaatattcta gttcataaaa
     4021 atacaaatat gtaaatataa tcaccttaat attcaattta tttaaaataa aaa
//
LOCUS       DAMKPG010000180         3964 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMKPG010000180 DAMKPG010000000
VERSION     DAMKPG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.186"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            1..391
                     /locus_tag="PC621_003593"
                     /pseudo
     CDS             1..391
                     /locus_tag="PC621_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC621_003594"
     CDS             complement(751..1905)
                     /gene="nagA"
                     /locus_tag="PC621_003594"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF4110702.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
     gene            complement(1988..2713)
                     /locus_tag="PC621_003595"
     CDS             complement(1988..2713)
                     /locus_tag="PC621_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF4110703.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            2982..3911
                     /gene="pfkB"
                     /locus_tag="PC621_003596"
     CDS             2982..3911
                     /gene="pfkB"
                     /locus_tag="PC621_003596"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF4110704.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
BASE COUNT         1444 a          550 c          562 g         1408 t
ORIGIN      
        1 tgcaaaagat taaaagaaag tatatgctgc aattacagag gaagtagcat taagtgagct
       61 atcgctctta gaggaaaaat ggggtaatca atatgcgata gcattaagaa gttggcgaag
      121 caattgaaat gagctagcaa catactttaa atatcctcaa gagataagaa cattgatata
      181 tactacaaat tcaatggaaa gttacaatag attattaaga aaagttacta aatcgaagtc
      241 tatttttcct ttagatgatt ctttgcacaa atcattatac ttagcaacta tggatatttc
      301 tgaaagatgg actcaaaaga ttagaaattg gactcaaatc ttagctcatt taagtattta
      361 ttttgaagaa agaattttaa ctctaaatta ataaagaaat atttcaaaaa aattaaggaa
      421 ttttcttaaa ggggtaataa tttttacaaa aatggaaaaa atatcaaaat aaggctatga
      481 tttttaaagc catagcctta ccaataaatg tttaattatc actaaaaaat aaatttttat
      541 tcaaagaata cacataaata tttccagatt cttaatttta taaacttata ttgtaaaata
      601 aagaacctta ataaaaggag tgtctcaaaa ttaacttttt agtttatgag gcactctttt
      661 tgtatgaaat caaatgtatt ttcatcattt taacaatgaa aaagagactt atctctattt
      721 ttaggaagcc tccataactt tatattgaat ctatttatta actttgtaca tacatatacc
      781 acttatataa gtttcgtata tattaaagtt cttatctgtt actataaagt ctgcatccat
      841 accttgatgt atggagccac agacattatc aatattacaa cttaaagcag gaacatatgt
      901 agccattttt attgcatctt ctaaagtggc tatgttccaa tcaactatat ttctcacagc
      961 cttgtccata gttaatacac ttccagctaa actcccacta tttaatcttg caacactatt
     1021 ttttacattt acactaaact taccaagagt atattttcca tcctccatag caccagccct
     1081 catacaatct gtaattaaag ctatgttatt actagacttc gcatccatca ttattttaac
     1141 agctattggg cttacatgat gaccatcaca aattaattca gcataagtat cttttagtac
     1201 catagcagcc ccaaccattc caggttctct atgatttaaa gggctcatcc cattatatgt
     1261 atgtacaaac actttagcac ctgaattaac gacttgttta acttgttcaa atgtagctga
     1321 actatggcca agagaaacag agatattatt tttagttgca tatcttgtta attctattga
     1381 acctttacgt tctggagcaa ttgctatttt tcttattaaa tttttcgatt ttctttgcca
     1441 taattttaat atattgatgt cagggtcttt aaaatatttt tcattttgag cgcccttata
     1501 tttttctgta aaaaatggac cttctaaata aatcccttgt atttttgcac cctcaactcg
     1561 attgaagcta ttgcttatat tttctacaga atcacttagt gtatctacag aatcagttaa
     1621 agttgtaggt aggaaagatg ttactccata ttttaataag ccttttgaaa ttgtgtttat
     1681 tgcttcaaat gtattgtcca tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac
     1741 taagccagga gcaatatatt tacctgaaaa atctatgaca ttatcagtaa tattaggtct
     1801 ttcttgttga aattcaccga atttgccatt aattatacta aggtaacctt tttctttgat
     1861 tccatgtttc ataaaaaatt tatctgcata aataaaactt ttcaaaataa cctccttata
     1921 attatactat cattgtggca ttgacgttta taatatcatt ctattttctt gaaagctatt
     1981 taattcacta tcttacatgt cttactctat atacaaattt atcgcttcta gcaacactca
     2041 aagtaaattc aataactata ttgtcatcat tatatgtggt acgaagtaat ttcagacaag
     2101 gtgaatctcc tggtacttcc aataacttag cttccttatc agagattatt ccagctgaaa
     2161 attcttcatc agcaacttta ataatctcgc cataatcttc tctaaatatt tcatataaag
     2221 gtttttgttc gattttttct tttgtcaatc ctataaattc tttcatagga agatatgttc
     2281 tttcaagcat cataggaagg tcatcagcaa gtcttaaacg cattattttt atcatttttt
     2341 caccaggagt tatacctaat ttttcagaga aatactttgt actttctact acctcaaatg
     2401 ataatacctt tgtacctggc ttttttcctt gtattctcat ataatctgta aagctataac
     2461 tttccattaa gttttgagaa ttttgactta gtgcagcaac aaaagttcct tttccatgtc
     2521 ttttgtatat atatcccata tgttccaact cagccatagc tagtctaact gtggttctac
     2581 ttactccata ttcatcacaa atttttcttt cagaaagcat tctatcgttt ggttccatct
     2641 cattttcaat cttagttctt aatatctcaa caagctggtc atatattggt cttttatcat
     2701 ttctttttat cataaaaaca tagtcctttc taagtggtta ttaccacaat ttgattatag
     2761 aatatagaat tatttatgtc aatgaataaa gtttaatcaa aattcaataa gtaaggctat
     2821 taatttatta taaaattatg gtatattgac aaaacaagta aaataattta tattcaaatt
     2881 ggtaataacc actttgggta tattatagat gaggtatttt ttattttaca gattaattta
     2941 ttgtttagaa aagtaaaaat taataatgag gagaatctag tatgattaca gtaataacat
     3001 tcaacccatc tatagataga atgtataaag tgaataatat aaacataggt gaggtgcaaa
     3061 gggtagtaag taagaatgca actgctggag gtaaaggatt aaacgtagct aaggtatgta
     3121 aaattttaaa agaaaatcct ttagctatgg gatttctagg tggatttaat ggagaattta
     3181 taaaaaatga acttagaaaa ctagatatac aaaataaatt tacaaaaata gagcaggaaa
     3241 ctagaaattg cttaaacata attgaggaca ataaagttag tacagaattt ttagaaaaag
     3301 gaccaatagt agaaaaaagc gatttagaga gatttgaaaa tgatatagag gaagtactaa
     3361 aaaatactaa aatactagta gcttctggta gttattgtga gaatatgccg atagattatt
     3421 atgaaaagat aggaaatata tgtagagcaa ataatataaa atttatatta gatacatcag
     3481 gagaagcatt aaaaatagct ttaaaatcaa aaccatattt aataaagcca aatatagatg
     3541 aaataagaca tttattagga ataaatatag aatcaagaga ggaaattatc ttgtcaggaa
     3601 aaaaacttat tgaaatgggt gcagagaatg tatgtatatc tttaggaaaa gatggtatgg
     3661 tgtatttaaa cgaatataat gtatatgatg taaaagtacc agttgtaaag tgtataaata
     3721 ctgtaggtag tggagatagc acagtggctg gatttagtgt aggtatttta aggggttata
     3781 aaatagaaaa tttacttaaa tttgcaaatg cttgcggtat aagtaatgct ttaaatatgg
     3841 aaactggatt tgtagatttg ggagaaattg ataaatatca agatttagtt gaagtaaaaa
     3901 agttaagtta aataaattta atgggaatat aataaattta atgttgttgt aatagatttt
     3961 aatt
//
LOCUS       DAMKPG010000181         3953 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMKPG010000181 DAMKPG010000000
VERSION     DAMKPG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.187"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(175..711)
                     /locus_tag="PC621_003597"
     CDS             complement(175..711)
                     /locus_tag="PC621_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF4110705.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(729..2042)
                     /locus_tag="PC621_003598"
     CDS             complement(729..2042)
                     /locus_tag="PC621_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF4110706.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2378..2818
                     /locus_tag="PC621_003599"
     CDS             2378..2818
                     /locus_tag="PC621_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF4110707.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3003..3191)
                     /locus_tag="PC621_003600"
     CDS             complement(3003..3191)
                     /locus_tag="PC621_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110708.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1379 a          663 c          380 g         1531 t
ORIGIN      
        1 attaatttat atagttttat ttattctaaa agtataatta taaaaataaa aaaagaatag
       61 gatatttttc ctactctttt ttctgtgctt atatataata ttaaagctct ttattttcaa
      121 tatatatttt aaattttctc tttttaatat atctttttct tttaataaat ctctttaatt
      181 ttcttcttct atatcagagc caaagggaat ccatgcatta ccaaactctt tatctttaaa
      241 taaataagct aatccagtta tttgcttcaa tctaagaatt tcatcaactt ccatacccag
      301 gtgctttgat atccattcat cagattttcc aatttcacaa agttcagtta taattttact
      361 cattaaatcc acattatgag aacctcttgc acgattgtgt cgaatagtac ttgccattct
      421 ttcatcaaga gatttatcaa ttactgaaac aggaagaagt ccgttttctc tatcatatat
      481 atccttattt tcaagcatta ctctatatct atgaaaacca tctacaataa tatacttatc
      541 cttttcctca ttataataac agacaattgg cattgtatac ccatcttctt ttatactttt
      601 atacaacaac tgcatttctg gtggggctac tgcatttgga ttatagtcgt tagattctat
      661 cttttcgatt ggcactgcga tgattccata tacaggactt ttatattcca tagcatctta
      721 actcctatct aatgaatttt ttcatatttc ttcttaatat attccgttct aattctctgt
      781 tcttttgtag gaccaaatcc cataaatcta cacatatggt cattttttaa aatacaataa
      841 cacattcttt tccaacttgg tatatctttg gtaaatttaa tatcatccgt atcatcaggt
      901 atatcatcca aaaatatgac tctactcttt ttatcttttg tatgatttga aactccattt
      961 aattctattt tataaccttt tctcaacaat tcacgtataa ctatttctga caaaccacca
     1021 cctgtattct gccaaaactc aatagatgtt gaaaattttt taatatagct attccttata
     1081 ttttcaggta aggtatcaag caaaaaatat gtgaaacttt tccatgtgtg tccatatggc
     1141 aatttaacac tacgatatcc catagctttt gtttttccat aaatagaagt aaaattaact
     1201 ccttgtactc tagcaaccaa tttcacccat acatctgggt ccaatattct gtataaattt
     1261 aaactctctt ttgctgaatc attaaaaggt gatgccactc tcatttttct tggagctact
     1321 ccagctttat agaataagtc ataaatttta ttatagtcat aatcaaattt atagtgggct
     1381 aaccatatat cttctatttc ccaatcatat ataggagatg ccgtccagac atttttaaat
     1441 tccttagtta tccagcactt tcctttatat ccatgctgtt tattcacaat agaacaataa
     1501 cgttgtagag attctgctgc tcgtgttcct aataagcaaa caacttttgc accttcatgt
     1561 atatctctaa tccatctacc aaattgcttt gccaaacttt cttgtctcat tttatactta
     1621 taataagtaa aaggattatt tttttgattt attatgtatt ctttgactgg catttctctt
     1681 acccataatt cttctttctc atcatcccat ggataccaat acatttcata atttcctaca
     1741 gcagttcttg tagccattgg taaacacacc caatatggtt caatatcttc aatattctct
     1801 tcaaatactt tttcaacata ttcagtagtc aaactatatt gagcctcaaa atcttgatga
     1861 aatactccta ttttggaact tatattattc tttctcttat aatccaaaac taaatttagc
     1921 agtaagccac tatcttttcc tccactaaaa gatacataga tataatcaaa ttcattaaaa
     1981 agataagaaa gtctttcttg aaaagcttca aatacatcgg attctaaata tctcttatcc
     2041 atttgaatta tcccctttca attagaagtt tataaaacct tacaatacaa tattattatg
     2101 cttttttagc agtaatcagc ataaattata cattatttaa atacacaaat ccaactaaac
     2161 acatataatt ataatgtaat tatacttttg tattataaac tatattttat cattatttta
     2221 catttttttc caatatatat aaatatcata tttttctatt agctaaaaat aattacttta
     2281 tatttttaca tgttagtata ataagacata aaaacttgac atactgttgt cataattact
     2341 ttgctataat taaaagtaaa aatggaggta aattataatg tatgaacgta tgcttgataa
     2401 aagcaataaa ccctgtttgt ctcaaataat agaatatata ggtaaagatg gatatgcacg
     2461 attgtgtaag ttggaaactt acttggaaac tcaatataat ttatcaagag aacttcgatt
     2521 tccttttgga aacaattatg gttggggtta caaatatagc cataaatctt ttcatttatt
     2581 ttatgctttt tttgaaaaag gtgcatttac tgttctgatt cagataggtg acaagcaagt
     2641 tccagcagta gaaaaaacgt tggtatcttt gacaccaaaa gctaaagaac tctgggtcac
     2701 tcgttatact tgtggcaata atggaggttg gattaagtat cgtattttag aagatgcaga
     2761 cttacttgat attattcaat atataagagc aaaaaaatct ccacctaaac aaaattaact
     2821 tttatttttt gtgatttaaa tatagtgtat ataaattcca ttctttccat attaaaaaaa
     2881 taatataaaa aatatctatt agtatatctt atttagtatg tcttattcta ctttttacag
     2941 ataattaaaa agttttatca taaataaatc ataactaaac atcctagtaa atcaaaattc
     3001 atttattctt ttttattaga atttttgata acagtcccta tacatatgcc aattatcatt
     3061 ccaaaaatta gaccataaca cattgtgata cccacatttc ctttagaaat acctatagca
     3121 caacctatag ccattccaag tatcatgcct ataaacatac atataataac taaattgttg
     3181 tttttattca tagtttttct ccattcaata tattttaaat tgcctagctc gccttcttag
     3241 cttaaaacaa agaaatataa attaactttc tttcattcta atatctgctt gtataaatgt
     3301 caaacaacac atttcttgtc ataacctcct ctttttgaag taagtacaat ctcccagaca
     3361 caataatatc aatttcctaa aaccatttaa gtaactttta tatttacata atatttacaa
     3421 tcataaacta tattgttatt catttataca tcataaacac tccctcctaa tcattattat
     3481 acttaaaata atgttcattt ataataatat atctaacgca ctttaattga aaaaactaaa
     3541 actttgcctt tcattcccat atataaataa gtatactttt tgatgctcta ttacaaatca
     3601 actaaattaa ttgatataaa ttatcatttt ctgttgaaaa taccatttac acatgctata
     3661 cttaattgca taaatggtaa taaatgtcat atgattttac aaatctatta ataacaatta
     3721 ttacttttac tgtattttaa agtttttgga ctaaatatca agaactttct aaaatacaaa
     3781 gtacaaaaaa tttatataag ttataaattt atatgaacta gataagctca aacaatatgt
     3841 tatgtataaa ctgtgtagta ttcggttata aatttatata aattatataa gctatataaa
     3901 gtattacctc aactatataa gctagatata taaattacat acataagcta gat
//
LOCUS       DAMKPG010000182         3939 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMKPG010000182 DAMKPG010000000
VERSION     DAMKPG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.188"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..239
                     /locus_tag="PC621_003601"
     CDS             <1..239
                     /locus_tag="PC621_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110709.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="PC621_003602"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="PC621_003602"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF4110710.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="PC621_003603"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="PC621_003603"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HDF4110711.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAMKPG010000183         3929 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMKPG010000183 DAMKPG010000000
VERSION     DAMKPG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.189"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            44..916
                     /locus_tag="PC621_003604"
     CDS             44..916
                     /locus_tag="PC621_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110712.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            913..2202
                     /locus_tag="PC621_003605"
     CDS             913..2202
                     /locus_tag="PC621_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110713.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2132..2776
                     /locus_tag="PC621_003606"
     CDS             2132..2776
                     /locus_tag="PC621_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110714.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3043..3507)
                     /locus_tag="PC621_003607"
     CDS             complement(3043..3507)
                     /locus_tag="PC621_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110715.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
BASE COUNT         1468 a          467 c          602 g         1392 t
ORIGIN      
        1 aaaataaaat tctataaaat tatttctggg ggtgtattta tatatggaaa tgaaaatagc
       61 agtatccaat cttagtaaaa gatttgggaa aaaacaagct cttgataata taaatcttga
      121 aatcgacagt ggtatgtttg gtcttttagg aagaaatgga gcaggaaaaa ccacatttat
      181 gagaatttta gccactttat tatcaaaaac agaaggttca atacatatgt gtggagttcc
      241 tattgagaat actaaggaaa ttagaaatat ggttggttat ttgccacaag atttttcaat
      301 gtatcctaac atgacagtat ataaagcaat ggattattta gcagttttat cggatatacc
      361 actaagaaaa agaagaaaaa ttatagcaaa gttattaagc aaagtaaacc ttactaatta
      421 tcaaaatgtg aaagtaaaat ctttatctgg aggtatgaat agaaggcttg ggatagcaca
      481 agcattaatt cataatccca gagttttaat agtggatgag ccgacagcag gtcttgaccc
      541 agaagaacgt ataagattta gaaatttact atgcgaaatt gcaaatgata gaatcgttat
      601 tttatctaca catattgttg gagatataga agctacatgt gaaaacgtag ctatattgga
      661 tagtggaaaa gtcatataca atggctcaac agaagcatta gcaaaactag ctgaagggaa
      721 agtatatagt attgaagtag ataaaaaaga cattgaaaat ataaaaagta gatttatagt
      781 tataggaatg ttgactcatg gtggaaaggc tatacttagg attatatcag atgataaacc
      841 ttttgagaca gcagtaaatt gtaatccaac tattgaagat ggctatatgc ttataatggg
      901 aggagataat atatgaatct atttataaaa gaatgtaaaa atatattaaa gagtatggta
      961 tattatattt ttattgtagt tttagttttt ttctatattt ctcaacttgg aagttcagtt
     1021 tcaaatgatg taagtaaaaa tattcctaat gatggaaatc cattagttgc tccacaaaaa
     1081 gtagagaata ttgacgaata ttactctgta cacaatcaat atccatatgg tcaaaaagaa
     1141 tctatgaaaa cagaaaaggt cattccaaat gcaactataa acctaattag agaatatcaa
     1201 aaaaacacat atacaacgta tccaacaggg gtagttaagt atataaaatt agaccaaaat
     1261 aaaacgaaga ccatagaaaa atatatagaa gaaataactg gtaaaagtat tagcgaaata
     1321 aatttaattt ggaaaactga tgaaagtata aaaatagatt attctaaagt atctcttgaa
     1381 agatttgaag aaataatgca aagagtagac gacattttag gaggagggtc tgcatatgct
     1441 tttgacaagt tacaaagatt tggagtagtt ccagttacct atgatgaagc tatgaagaat
     1501 tacaactata caatacaaaa agataaagtt agtaatgggt atgcaagaat tttttgcgat
     1561 tatataggta tagttttaag tatatttcca gtatttgtgg cagtttttat gtcacttaaa
     1621 gataagcgtt caagaatgag agagattatt tattcaaaac aagtatcttc atgcaaaata
     1681 gttttatcaa gatattttgc acttgtcttt ataatgatat tgcctgtatt aattttaggt
     1741 atgaaagatt tacttccttt gattttcttt gctaaagata aaggtatatc tattgataac
     1801 ttagcattta taaaatatac attatggtgg atactaccaa ctcttatgat agttacagct
     1861 gtaggaatga gtattactat attaacagat acaccagtag caattattgt gcaattaatt
     1921 tggtggcttt acagtataaa ttctacagga ctcaaaggtg attattcaac aatggggcta
     1981 atgataagac ataatagtgt attaaatggg gatattatta tacaaaactt caatcaaatt
     2041 atgattaata gaattgtgat aagcttggga gcattggtgc ttgttttgtt agctatattc
     2101 ttatatgagc aaaaaagaag ggggaatctt gatgtttggg caaagttttc aaaactgttt
     2161 agttctaata aaagaaaact tcaaattaag cactttaact aatttaatat gttcaattat
     2221 attgctatgt atgattcctc taatacaagg agtagctaat ttagacagaa tttcaacagc
     2281 tatatgttta gaaaattatg ttgcaataat agggatagta atgttggtac ctgtattttc
     2341 gctagagcaa tcaaaagaaa ttgatgagat agtatcttca aaatcattat ctcaagttat
     2401 tgtacaatta attaggatac tgatgtcaat tataggtata gtggtattaa tcataggttt
     2461 tgtaaatgtt ttaatatatc ttggatgtaa ttttccagta gttgagtata ttattggaag
     2521 tttcgcatca gctatgttta taggaagttt aggaatgttt ttctcaaatt tatttaataa
     2581 tacaatcata ggttacatga tatctatagg atatttaatt ttaaatatga tgactaaagg
     2641 caagtatgtt ggaaaatttt ttataatgtc tatgagtcaa ggtagttttg atgagaaata
     2701 ttggctgata cttggaagtg ttgtgttgat aattgcatct ctcttaataa aaccaataaa
     2761 aaggaagatt aggtaataaa atgagagctt agataaaata aaggtaatac aaataaaaag
     2821 tacatttatg aaatataaat ttatttttat atacacaaac ctacttacaa tctctttatg
     2881 tatgtagata gatttatata tctgtagtat aaaatatata tgtataccga attttatcaa
     2941 aggctctcaa atttatattt caaagacact tattgctatg acatacacac atgtattttt
     3001 tcattttaat tgtcatatta gcaatatgac tagtgttata aactattgat acctcataac
     3061 aaaaaattct ccatccccta aatgcgtttt ctgtacctga gtctcaacta ttttaaatcc
     3121 ttgagactta taacatttta cagctctcat gttccaggtt cttacattta atacaattgg
     3181 tttagtacta tatttatttc tacattcttg taatcctagg ctaattattt ctttgccaat
     3241 tcccattcca caataatctg gttttatacc aataccaaag aatacataac ttccttcatc
     3301 caataagttt ataaaaccta tcaattcttt attgtcattt ataaaactta taaaattttt
     3361 tcttttatct tcttttgata gtgaaaaatt cttcttttta atttcctccc atggaggtag
     3421 gttgtatatt gaatatttac cttcatattt ccaattagta acttcaatag catgttcttc
     3481 agtaaattta actttattta aattcatatg tcctcctatt aaaatattta aggtaattaa
     3541 tggtatttta aattttacaa aataagtaaa actatttata tctatccata aaatctttaa
     3601 aaattttact aacaattctt gtaccctgta ctttttattg gattcacaga agactataaa
     3661 ctaattttga attaccttct aattcaaaaa catcacgata tatgtaattt cttagcttga
     3721 ttaaattctg ttaaatctgt ttcttatagt tgtataatca acatcaatat ctttatgtgt
     3781 attagttaat ttattggttt taggaactta ttattaatat attgtatttg ctccaatact
     3841 ccaagtagta ggtaaattat aggcaaatta tcaaatgtat atctatccat gcttaaactt
     3901 tagagataca ataactacta aattagtat
//
LOCUS       DAMKPG010000184         3926 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMKPG010000184 DAMKPG010000000
VERSION     DAMKPG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.190"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            183..1673
                     /locus_tag="PC621_003608"
     CDS             183..1673
                     /locus_tag="PC621_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF4110716.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1931..3427
                     /locus_tag="PC621_003609"
     CDS             1931..3427
                     /locus_tag="PC621_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF4110717.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3561..3911
                     /locus_tag="PC621_003610"
     CDS             3561..3911
                     /locus_tag="PC621_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4110718.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         1560 a          398 c          767 g         1201 t
ORIGIN      
        1 agtaaaaata aaaatagtga aatttcagaa atatcaataa tattatataa taaaataaaa
       61 aaacactata tatattcgat aaaataaaat ttaaacatat aatattttat gtatatatta
      121 ataaaaggac atattgtgtt ataatggtat cgtataaaat catttacggg ggtggtatta
      181 aaatgtttca atttgaaaca caaatacaca aattaagaca tgatgtttta actgcaatag
      241 ctagacttgg aaaagaagat aaccttacca gagaagcaat ggataatatt cagtatgaaa
      301 taataaaagg tgagaaacct acatacagat gttgtgttta taaagaaaga gctataatat
      361 ctgagagagt aaatgtaagt atggggttac aaccaggtca agataagata gatttagata
      421 caatggatat agaagaagat gaacaaatta tatatatttt ggaagctgca tgtgacagtt
      481 gtccaataaa tgaatttaca gtaacagata tttgtagagg atgtctagca catagatgta
      541 aagaagcttg taaatttgga gctataacac atgtcggagg aatggcctat ataaatcatg
      601 aattatgtaa ggcttgtgga atgtgtaaaa aagcttgtca atatgatgcc atctcagagg
      661 ttgttagacc atgtaagagt gtttgtccaa ctaatgcctt aggatttgat agagaaaata
      721 tgaaagctat gatacatgag gataaatgtt taaactgtgg agcatgtatg tcagcatgtc
      781 catttggagc aatatctgat aagagtttga tagctcctgt agctagaaag ttggttcaaa
      841 aagagaaaat gtatgcggta gtagctccag ctataacagg tcaagttgaa gcaaatgtta
      901 catatggtca aataaaaaat gctataaaat cactaggatt tgttgatgta ttagaagcag
      961 cttgtggagc agatgcagtt acagttcatg aaagcttaga atttgctgaa agaatggaaa
     1021 agggagaggc ttttatgaca aattcttgtt gtccaggatt tgttggttat atagaaaaaa
     1081 catacccaaa tcatgtagga aaaatatctt ctacagtgtc tcctatgata gctagtggta
     1141 gatttataaa aagtcatgaa ccagatgcta aagttgtatt cataggacca tgtacagcca
     1201 aaaagagtga ggcagctaag aaagaattaa aaggagctgt tgattatgta cttacttttg
     1261 aagaaataat ggcattattt gaggcatttg atatagactt ggctaagtgt tcaaatgaag
     1321 atatagatga tgcatcaatt tttggaagag gttttggagc tgctggtggt cttacaaaag
     1381 ctatagaaaa ttatcttgct gaaaaaggta ttggtgttaa ttttgaacct gtaaagattt
     1441 ctggttcaag agaaatcaag aaaactatga ctatggctaa tttaggaaag ctagaaggta
     1501 attttataga aggaatgatg tgtgaaggtg gttgtataaa tggttctggt aaattcttat
     1561 caggtgcaaa ggttaaagcc acttttgata gaaaaaattc tcaatcaact aagaaaagtg
     1621 tcttagcaaa tacaaagata aaagatttta gtaatataaa tttagaaaaa taaagataat
     1681 acaaagcaac aaaaatacat attgttaata gagttgttct aagttgaggt ttaacttttg
     1741 gacaactcta tttttattta tatatttaat taatgaacaa ctaaaataaa aattacatat
     1801 ataatatttt gtatataact taacaaagta tatgcctatg ttataataat ttatagaaat
     1861 aaataaaaaa tacaatatac ataatagtag aaatatagta aaaaggtgtg atttataggg
     1921 aggattactt atgtttgaat ttgaaacgca actaaaaagg ttgaaacatg aagttttaac
     1981 agcagtagca agactatcta ttgaagaaaa tttaacaaaa gaaaatatag aaaaaattcc
     2041 atatgaaata attaaaggag aaaaggcgag atatagatgt tgtgtttata aagagagaga
     2101 agtagttctt gaaagggcgc aacttgcatt aagtttaact ccaaatagta aatatggaga
     2161 tataaatcca gattcagtta gtttggacac agatgagcaa attatatata tagttgaagc
     2221 agcatgtgat agatgtccaa taaatgaatt tacagttaca gagctttgta gagggtgttt
     2281 ggctcataga tgtaaggaag catgtaaatt tggggcaatt tcttatataa atgggagagc
     2341 atatataaat catgatttat gtaaggcttg tggaatgtgc aaaagttcat gtcaatatga
     2401 tgctatatca gaagttgtaa gaccttgtaa aagcgtttgt ccaacaggag ctttggattt
     2461 taatagaaac actatgaaag caatgataca tgaagataat tgtataaatt gtggagcatg
     2521 tatatcagca tgtccttttg gagcaatatc tgataagagt ttgatagcac ctgttgcaaa
     2581 aaaacttgca aagaaagaaa atatgtatgc aatagtagct ccagctataa caggtcaaat
     2641 agatagtaat attacatatg gtcagttaaa aaatgctata aaatcacttg gatttgtaga
     2701 tatgatagaa gcttcttgtg gagcagatgc agttacagtt catgaaagca atgagcttat
     2761 tgaaagaata aaatcaggag ataattacat gacaaattca tgttgtccgg gattttttaa
     2821 gtatatagag aagatgttcc cagatgaaaa agatagaata tcatctacag tgtctcctat
     2881 ggtagcaaca ggaagattag taaaaaaaat agattctgat gctaaagttg tatttatagg
     2941 gccatgtaca gctaaaaaga gtgaggcaac agagaaaagt ttaaaagatt cagtagatta
     3001 tgtacttact tttgaagaac taatggcatt atttgaagca tttaatatag atttatcaaa
     3061 atgttcaaat gaagatataa atgatggttc gatttttgga aggggatttg gagcgtctgg
     3121 tggacttgcc aaatcagtac agaactatat tgctgaaaag ggacttagtt ttgagtttga
     3181 acctataaaa gtatctggtc caagacaaat taaaaagaca atgacaatgg ctaaattggg
     3241 aagattagaa ggtaatttta tagaaggaat gatgtgtgaa gaaggttgta ttaatggagc
     3301 aggtaagttt ttatcaggtg taaaagctaa aaatactttt gatagaaaaa atggacaatc
     3361 tactaaaaag agtgttttat ctaatgataa gattaaagaa tttgaagatg taaatttaga
     3421 aagataattg ttttggtaca gtcatttttt tatcatgcca caaatatgcc taaaattttc
     3481 cctttttata tgtgtatttt acagagttaa ggttgctata ctataaatat gtaataaata
     3541 aaatttatag gagcttttgg atgaagaaaa tagtattaaa agatatcctg tttttagtta
     3601 ctacattatc tataatagta atattattgt ttgtaacaaa tataactact aatagtaaga
     3661 taaaatcaac aagtgaatct tatttaaaaa gtgacaaaaa tagtgtgagg caaaatggta
     3721 tccagaggag cacaaaaaat actgcattta agactactat attaagtact ggaaagtctg
     3781 actgtatatt aatcgagata ggtgataaat tcataatgat tgatacagga gaagataaaa
     3841 atggagaaca aatagttgat aaattaaaag aaaaagaaat aaatgcactt gactatttga
     3901 ttaaaaaata gctacgcaca ctcgtg
//
LOCUS       DAMKPG010000185         3812 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMKPG010000185 DAMKPG010000000
VERSION     DAMKPG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.191"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            176..736
                     /locus_tag="PC621_003611"
     CDS             176..736
                     /locus_tag="PC621_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HDF4110719.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
     gene            complement(1071..>3812)
                     /gene="tcdA"
                     /locus_tag="PC621_003612"
                     /gene_synonym="toxA"
     CDS             complement(1071..>3812)
                     /gene="tcdA"
                     /locus_tag="PC621_003612"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF4110720.1"
                     /translation="KSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLF
                     YFDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIINGKHFYFNNNGVMQLGVFKGP
                     DGFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFN
                     PNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGK
                     HFYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNN
                     SKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYY
                     FNTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGFEYFAPANTHNNNIEGQAIL
                     YQNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNNAIAATHLCTINNDKYYFSY
                     DGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQ
                     NKFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAVAVTGWQTIDGEKYYFNLNT
                     AEAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFNTDGIMQIGVFKGPDGFEYF
                     APANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVA
                     VTGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPA
                     NTDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWATIDGNRYYFEPNTAMGANG
                     YKTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGKI
                     YYFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         1369 a          565 c          528 g         1350 t
ORIGIN      
        1 attttaagtt tttaaatttt aaaataaaat atatctaata aaagggagat tgtattatgt
       61 tttctaaaaa aaatgagggt aacgaattta gtaatgaaag aaaaggaagc tctaagaaaa
      121 taattaaatt ctttaagagc acaaaggata ttgctctact ggcatttatt ttggtgtgtt
      181 ttttggcaat atatcctcac cagcttgttc tgaagaccat gaggaggtca tttctaatca
      241 aacatcagtt atagattctc aaaaaacaga aatagaaact ttaaatagca aattgtctga
      301 tgctgaacca tggttcaaaa tgaaagacga cgaaaagaaa gctattgaag ctgaaaatca
      361 acgtaaagct gaagaagcta aaaaggctga agaacaacgt aaaaaagaag aagaagagaa
      421 gaaaggatat gatactggta ttacttatga ccaattagct agaacacctg atgattataa
      481 gtacaaaaag gtaaaatttg aaggtaaggt tattcaagtt attgaagatg gtgatgaggt
      541 gcaaataaga ttagctgtgt ctggaaatta tgataaggtc gtactatgta gttataaaaa
      601 atcaataact ccttcaagag tgttagagga tgattacata actataagag gtataagtgc
      661 tggaactata acttatgaat caactatggg tggaaaaata accataccag ggatagctgt
      721 agagaaaatt aattaatatt gttttgtatt atagttaata ttttatatta tagtcaatat
      781 gtttaaagat gtttttataa ttgcaaataa acagttacaa ggctctaaat tagtttttgc
      841 ttttagcata ttatctattt tctatcaact attaattatt tagtattaat atttccatat
      901 atgaatttta ttataaaata gtcaagaata atagattatt aaatgataga aaaattttaa
      961 ctaaaagtca tgtattacaa taacacatga cttttaatta aatctcaata tttattatat
     1021 aaaaataatt tctgagtatc acaggaataa ttttttgtca aacatatatt ttagccatat
     1081 atcccagggg cttttactcc atcaacacca aagaaatata taacaccatc aatctcgaaa
     1141 agtccaccag ctgcagccat agcagtatca ggcataaagt aatatacttt actattaata
     1201 gtttgccatc cagtaactgc ttttgagtta ttaccaaagt aatatatttt tccaagtaaa
     1261 tgtaggaatc tattttgata acgtatagct tgaccatcta tattgttagc atccgtatta
     1321 gcaggtgcaa agtattcaaa tccattaggt cctttaaaca ctcctatctg aggtaaacca
     1381 tttctaaagt aaaaattttt attatcaata gttttataac cattcgcacc catagctgta
     1441 ttaggctcga agtaatatct attaccatca atagttgccc aaccagtagc cgcttttgaa
     1501 tcattgccaa agtaatatat attgtcatgt aaatatagga atctattttg ataacgtata
     1561 gcttgacctt ctatgttgtt agcatccgta ttagcaggtg caaagtattc aaatccatca
     1621 ggtcctttaa acactcctat ctgcataata ccatcagtat taaaataaaa atgtttacca
     1681 ctaataattg tataaccagt tgaagctata taagtgttag tattaaagta gtatttttta
     1741 ccattaatag tttgccatcc agtaactgca acagcagtgt tagtattaaa gtaatatttt
     1801 ttaccatcaa tagttcgcaa tccggtaact gcttttgagt cactaccaaa gtaatatttt
     1861 ttaccattca aagttaagaa tttattttgg taaagtatag cttgaccttc tatgttatta
     1921 ttatgagtat tagcaggtgc aaagtattca aatccatcag gtcctttaaa cactcctatc
     1981 tgcataatac catcagtatt aaaataaaaa tgtttaccat taataatcgt ataaccagtt
     2041 gaagctatat aagtgttagt attaaagtag tatcttttac catcaatagt ttgccatcca
     2101 gtagctgctt cagcagtgtt aagattaaag taatattttt caccatcaat agtttgccat
     2161 ccagtaactg caacagcagt gttaagatta aagtaatatt ttttactatc aatagtttgc
     2221 catccagtaa ctgcttttga gtcattatca aaataatatt ttttgccatt caaagttaag
     2281 aatttattct ggtaaactat agcctgacct tctatgttat tattatgagt attagcaggt
     2341 gcaaaatact caaatccatt aggtccttta aatactcctg ttaccatttt agattcatta
     2401 ttagcatcaa aatagaaatt atttctttca atagtaatat atccattttg aagaattcca
     2461 tcataactaa agtaatactt gtcgttattt atagtgcata gatgagtcgc agcaatagca
     2521 ttattaggat taaagtaata ttttttacca tcaatggttt gccatccagt aattgctttt
     2581 gagtcactac caaagtaata ttttttacca ttcaaagtta agaatttatt ttggtaaagt
     2641 atagcttgac cttctatgtt attattatga gtattagcag gtgcaaagta ttcaaatcca
     2701 ttaggtactt taaacactcc tatctgcata ataccatcag tattaaaata aaaatattta
     2761 ccattaataa ttgtataacc agttgaagct atagaagtgt tagtattaaa gtaatatttt
     2821 ttaccatcaa tagtttgcca tccagtagct gcttcagcag tgttagtatt aaagtaatac
     2881 tttttaccat caatagtttg ccatccagta gctgcttcag cagtgttagt attaaagtaa
     2941 tattttttac tatcaatagt ttgccatccg gtaactgctt ttgagttatt atcaaagtaa
     3001 tattttttac cattcaaagt taagaattta ctttgataaa ctatagcctg accttctatg
     3061 ttattattat aagtattagc aggtgcgaaa tattcaaatc cattagagcc actaaacaca
     3121 cctattttca ctacacaatc actatcaaaa taaaagtgtt taccatcaat agttttataa
     3181 ccattaaagg caatagcggt atcagtatca aagtagtatc tactaccatt aacagtctgc
     3241 caaccttttg agatgatagc agtgtcagga ttgaaataat acttattatt gtcaattact
     3301 tgcaatccga ctgcagcaat agcattatta ggattaaagt aatatttctc attgttaata
     3361 atcctccatc cagtgactgc ttttgagtca ttatcaaaat aatatttttt gccattcaaa
     3421 gttaagaatt tactttgata aactatagcc tgaccttcta tgttattatt ctgagtattg
     3481 gcaggtgcaa aatactcaaa tccatcaggt cctttaaata ctcctaactg catcacacca
     3541 ttattattaa aataaaagtg tttaccatta ataattttat aactagttga agctgctcca
     3601 gtatttatat caaaataata ttttttacca ttgatagttt gccatccagt tactaagtta
     3661 gattctatag gatcaaaata gaataatgaa ttatttatat tgattaatcc tttaactaat
     3721 ttactatctt catcatagta ataagtttta ttatctatta ttttaaatcc taaatgatct
     3781 ctatctaatt cattttcaga attaaatgat tt
//
LOCUS       DAMKPG010000186         3620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMKPG010000186 DAMKPG010000000
VERSION     DAMKPG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.192"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..1431
                     /locus_tag="PC621_003613"
     CDS             <1..1431
                     /locus_tag="PC621_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4110721.1"
                     /translation="ILILLTLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDE
                     NFLKHVQNYIISNEKENCVMACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKE
                     AIYGKLKSDVFVLIIKLENREQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYK
                     FNNNEIDIKKAIDNADMARLKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNK
                     EFVIHYQPKVDSSTGNIIGSEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQ
                     EVFITINKWISEGINVLPVSINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITET
                     TALRDVKFISERLYEIKSLGMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVT
                     NTKTKFMVKAIINLSHDLNVTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNY
                     KKLLTDGSYINLKDTLLDKCMTVEEE"
     gene            1434..1832
                     /locus_tag="PC621_003614"
     CDS             1434..1832
                     /locus_tag="PC621_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF4110722.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1872..3605
                     /locus_tag="PC621_003615"
     CDS             1872..3605
                     /locus_tag="PC621_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF4110723.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGVGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
BASE COUNT         1525 a          299 c          605 g         1191 t
ORIGIN      
        1 attttaattt tattaacttt gtttagtata aatgtattta tttcatatct taaaattaag
       61 ttgtatgagg ataaaattaa tttaggattg ataaataatt cagataagtt tataaatatt
      121 actaaagatg agaacttcct aaaacatgtt caaaattata ttatcagtaa tgaaaaagaa
      181 aactgtgtaa tggcttgttt tgatttatgt agactcaaac ttataaatga tatatatggt
      241 tatgagctag gggatgaagt gttaaataat attttaagta acttaaaaaa ttattttgga
      301 aaagaagcta tttatggaaa gttaaaaagt gatgtttttg tattaataat aaagcttgaa
      361 aatagggaac agaagattcc ttatttagta aatcttatca agaataaaat tatgatatta
      421 agtcagattg acagttttaa aatggatata aatattgagg cttctattgg aatttataaa
      481 tttaacaata atgaaattga tataaaaaaa gctatagata atgctgatat ggcaaggctt
      541 aaatcaaaag gactaaagca catagagtat gtagaatttg ataatgctat ggaagaagaa
      601 atacaatcaa ttatgaaaat agagagagat ttattcttgg ctattaaaaa taaagagttt
      661 gtaattcact atcaacctaa agttgattca agtacaggta acattatagg ttcagaggca
      721 ttgataagat ggttgcaccc atcattaggc atggttggac caaataagtt tatacctata
      781 gctgaaaaaa atggtctgat aaacaatatt ggtagatggc taattcaaga agtttttatt
      841 accattaaca agtggatttc agaaggtata aatgtcttac cagtctcaat aaatctttct
      901 agagtagaac tatataaaaa tgatttagtt gattttttta aactgatgtt taatacatat
      961 aatattccaa aagaacttat agaattagaa attacagaaa cgacagcgct aagagatgta
     1021 aaatttataa gtgaaaggct ttatgagatt aaatctttag gtatgaatat atcattagat
     1081 gattttggag taggtaattc aaattttatc aatcttaagg ggataccact agatattata
     1141 aaaattgata ggtcacttat attggatata gttacaaata caaaaacaaa gtttatggtg
     1201 aaagcaatta ttaacctttc acatgattta aacgtaactg ttatatgtga aggggtagag
     1261 gatatgcatc aggtcaaagt cttaagtgaa ctgggatgta atttcataca aggttatgta
     1321 ttttttaaac ctttggatga gatgaattat aaaaaacttc ttactgacgg aagttatata
     1381 aatctgaaag atactctttt agataaatgc atgacagttg aggaggagta aggatgattt
     1441 cagaagatgt agattattca ataagaatca taagagctat atttaataat ggagaagata
     1501 aaccaataga tgctaaaatg atattaaaaa gtgaagatat accaatacta acactataca
     1561 gactgcttag aaagctttgt gcaaacaata ttcttacttt taacaaagaa gatggcaaga
     1621 catacatgtt aaatgtgtca ttggatacaa taagtctaag aactattgta gaagtaattg
     1681 atggaaaaat atatataaat aaatgtacga gatatgtatc taattgcagt aattgcttaa
     1741 aaggttgtaa agtaaatcgt gaattgctag aaatagaaaa agaaatttat aaacttttat
     1801 cgagaaaatc tataagacaa atactagaat aaaaaatttt cagctatgat aataattgga
     1861 ggttttcggt tatggatgat atatctcaag ataattttct actgtctaaa gagtatgaga
     1921 atagcttaga tgtagatact aaaaaagcaa gtggaatata ttatactcct aaaataattg
     1981 tagattatat tgtaaaaaaa actttaaaaa atcacgatat aattaaaaat ccatatccac
     2041 gaatacttga tatttcctgt gggtgtggaa attttctttt agaagtatat gatattttat
     2101 atgacttatt tgaggaaaat atatatgaat tgaaaaagaa gtatgatgaa aattattgga
     2161 ctgttgataa catacaccgt catatattaa actattgtat atatggtgct gatattgatg
     2221 aaaaagctat aagtatatta aaagatagct taacaaataa aaaggtagta aatgatttag
     2281 atgaatctga tataaagatt aatctatttt gttgtgatag tcttaaaaag aaatggcgat
     2341 ataagtttga ttacatagtg ggtaatccac cctatattgg tcataaaaaa cttgaaaaga
     2401 aatataaaaa atttctccta gaaaaatata gtgaagttta taaggataaa gctgacttat
     2461 atttttgttt ttataaaaaa attatagata ttttaaagca aggaggggtt ggcagtgtta
     2521 taactccaag atatttctta gaaagtctat caggtaaaga tttaagagaa tatattaaat
     2581 caaatgtcaa tgttcaagaa atagtggatt ttttaggtgc aaatatattt aaaaacatag
     2641 gggtatctag ttgtattcta acttttgata agaaaaaaac aaaagagaca tatatagatg
     2701 tatttaaaat caaaaatgag gatatatgta taaataaatt tgaaacattg gaagaacttc
     2761 taaagagtag caaatttgaa cattttaata taaatcagag attgctatct gacgaatgga
     2821 tacttgtaaa taaagatgat gaaacttttt ataataagat acaagaaaaa tgtaaatata
     2881 gcttggaaga tatagcaata agttttcaag gaataattac agggtgtgat aaagcgttta
     2941 ttttaagtaa agatgatgta aagttgaacc ttgtagatga taaattttta aaatgttgga
     3001 taaaaagtaa gaatataaat aaatatatag tagataaaag tgaatatagg ctaatatact
     3061 caaatgatat agataatgag aatacaaata aaaagatact agatgaaatt atagggctat
     3121 ataaaacaaa attagaaaac agaagagaat gtaaaagtgg aataaggaaa tggtatgaac
     3181 ttcaatgggg tagagaaaag cttttctttg aaagaaaaaa gataatgtat ccttataaat
     3241 caaatgaaaa tagatttgct atagattatg ataacaattt ttcaagtgca gatgtatatt
     3301 ctttttttat aaaagaagag tatttagata aattttcata tgaatatcta gttggaattt
     3361 taaattccag tgtttatgat aaatacttta aaataactgc taaaaaaatg agtaaaaata
     3421 tatatgatta ctatcctaat aaggttatga agataaggat ttttagggat aataattatg
     3481 aagagataga gaatttatca aagcaaataa tatcaatttt attaaataag tctatagata
     3541 aaggaaaagt agaaaaattg cagataaaaa tggataactt gataatggat tctttaggta
     3601 tataagcagt tgtaagtatt
//
LOCUS       DAMKPG010000187         3594 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMKPG010000187 DAMKPG010000000
VERSION     DAMKPG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.193"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(109..960)
                     /gene="gatY"
                     /locus_tag="PC621_003616"
     CDS             complement(109..960)
                     /gene="gatY"
                     /locus_tag="PC621_003616"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF4110724.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1009..2178)
                     /locus_tag="PC621_003617"
     CDS             complement(1009..2178)
                     /locus_tag="PC621_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF4110725.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2640..3557
                     /locus_tag="PC621_003618"
     CDS             2640..3557
                     /locus_tag="PC621_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF4110726.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
BASE COUNT         1222 a          576 c          428 g         1368 t
ORIGIN      
        1 tttcatcaat taaatgaaaa aaatacacca tttacatata attaatataa atttttcata
       61 taacaaatat cctaaactta tctaaaataa atttaggata ttttattttt atactctatt
      121 tacagaccca cacactttaa tcttttcttt tacaaaatcc ttcactgatt gaagccctgc
      181 taatccatac tttttagggt ctataacctc agaatcttca tctaaaactt ttttaacccc
      241 tttactatat gcaattctta attcagttgc atagttaact tttgcaatac ctctttttat
      301 agcttctttt ataatatcat catgtattcc tgaacctcca tgcaatacta aaggtattga
      361 aactacattt ttaatttctg ataacctatc taaatctatt ttaggttcac ccttatacaa
      421 tccatgtgca gtacctatag caatagccaa tgaagatatt cctgttctat cgacaaactc
      481 ttttgcctct tttggatttg tataatcatt actattcccg ccatctaaat catcttcttt
      541 tccaccaact ttcccaagtt ctgcttccac tggtataaga gaaggactac atgcttttac
      601 tactgattta gatatagata tattattttc aaagctttca tgagaaccat ctatcattat
      661 tgatgtatat ccacttctta aagctttcat tgcaaggtca aaacttgaac catggtccag
      721 atgcattgct acaggtatct ttactctttc tgcagcaacc tttgcatttg ctagataata
      781 atcaagacct gcatatttta cagtagatgg agtagtttgt aatattactg gagagtttaa
      841 ctcctcagct gcttctataa cagcaattac catttccata ttttctacat taaatgctcc
      901 tactgcatac ccctcatctt gtgccttttt aagcattgat gttgttgtta ctaatgccac
      961 attgataccc tcccaaatta tttagttata atattttaat cttatctttt aatattcata
     1021 tattgttaca ccttttacta ctctatttac tgttcctgta gctgatggcg tatctggctt
     1081 atttccaacc tttaccgata caaacaatgc tatagtttgt gcaaacaata tgtatggcat
     1141 tgcaagatac acatctggta atttattata cttttcttta aactcaaact ttgttcctga
     1201 gaagttattt tcattttcta ctgacactgc acaagtgaat ttagcaattt tatccctttt
     1261 tatctcctct aaaacatcta aatcatattt tcttgtatat aaacagttag atacaaattc
     1321 aaatacaagt gtattttcat caataaatga ttttggtccg tgtctaaacc ccataggaga
     1381 atcatacacc gtagatattt ttcctgctgt aagttctaaa agttttagtt gtgcttcttg
     1441 tgtcaaacca cctaatcctc ctgaacctaa atatacaact ctatcgaaat ctttatttat
     1501 taactcatgt atttcatctt ttctatctat aacatttctt cccatttcaa ttattgcatt
     1561 tatatatgac ttttctacat catcttctaa actatcgaat attaacatag cagataacat
     1621 catacacgaa aaacttcctg tcatagcaaa gccttcatca tttgattttg atggcattaa
     1681 taacaagtaa ttattttcgt cgtttttagt cattttagct agttctcctt gcggattaca
     1741 agttatagct aaatgataaa aattatctac aatcttattc cctaaattta aagcagccaa
     1801 actttcagga ctatttccac ttcttgcaaa tgaaattaat aatgttggta tatctttttt
     1861 taagtaatca tatgggtttg aaactatatc cgtagtaggt attgcttcaa atatgtattt
     1921 tcttatgtca tttttattct taaggtaagg aagtattgaa tttcctatgt aagctgaagt
     1981 tccagcacct gtaaatataa ctctaaactg cccatgatta ttacttattg tatctatgaa
     2041 tctatttaat ttttctttgt tagatttata tatttcataa gtctctaacc ataactcagg
     2101 ttgttgtttt atttcattag ttgttattat tgcacctaaa tcttttagtt tttgttcttc
     2161 taatttaaac attttcattc tcctttcata cttgtaacgt ttacatttgg taatgaggtt
     2221 ataaccactt taaactataa aaaaatatac tcttttataa ttttttgctt agatttttta
     2281 gtactactta aactcactat aaaatatacg ctatagattt taattaattt tatagtttat
     2341 ctttaaaaat ttaataaaat catactttag tatttttatt ttacgataaa aacttgttca
     2401 gaccaaagtg gtaattacca ctaatccaaa tataaattaa tttgaatttt ttgtcaatac
     2461 ataaaatatt tatatcaatc aattttatag atatcctaca aaattacttt gtcaatattt
     2521 cttatttcgt taatttactt atataagctt taaatttatt ttttgataca aagtttacaa
     2581 atttatgtgt ctgaattata atcaattctg taaatataaa aaccttagga gaaagtatta
     2641 tgaaaaatat aaaaatggga atgataggtc ttggctccat agctcaaaag gcatatttac
     2701 ctatattaac taagtctgag agatttgagt ttgtaggagc ttttactcca aataaagtta
     2761 aaagggaaaa aatctgtagt gattatagaa ttatgccatt cgattctatt gaatccctag
     2821 ctaaaaaatg tgattgtatt tttctacata gctctacaga aacacactac gaaattataa
     2881 aaatattatt aaatttaggt gttcatgtat atgtagacaa acctcttgct tcaacagtaa
     2941 atcaaggaga agaacttatc gagttaagta agaaaaaaaa cttaaattta atggttggat
     3001 tcaaccgtag attctgtcct atgtacaaag aaattaaaaa taatgctaca gaaatcgtat
     3061 ctatcaatat atgtaaacat ggattaaatt ccttaagaaa tgttagattc gacagtaccc
     3121 ttatagatga ctatatacat gttattgaca ctgctttatg gttagcaaat gaagatgttg
     3181 aaattagcgg agaagattta tttttaacag acaataagaa tcttatattt gtaagtcata
     3241 aacttaaagg taaacatttc tctgtaaaca cctctatgca tagagattct ggaacaaagt
     3301 tagaacaagt tgaaattctt tcaaaaggta agattcaaag agttaagaat cttaatgtac
     3361 tggaaataga agaaggtgga aacctaactt taaaacaaag tggtgcatgg gtaaatatat
     3421 taaaacaaaa gggttttgag gatatatcaa atcattttat tgattgtata gaaaataata
     3481 taaaaccagc tattaatgga gaagagtgta tcaaggctca aagattacta gagaaaatta
     3541 taaattcagt aaaatagata tttattataa aaataggtta aaaatgaatt tttc
//
LOCUS       DAMKPG010000188         3528 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMKPG010000188 DAMKPG010000000
VERSION     DAMKPG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.194"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            136..1734
                     /locus_tag="PC621_003619"
     CDS             136..1734
                     /locus_tag="PC621_003619"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF4110727.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1969..2160)
                     /locus_tag="PC621_003620"
     CDS             complement(1969..2160)
                     /locus_tag="PC621_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF4110728.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2329..2922)
                     /locus_tag="PC621_003621"
     CDS             complement(2329..2922)
                     /locus_tag="PC621_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF4110729.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2923..3069)
                     /locus_tag="PC621_003622"
     CDS             complement(2923..3069)
                     /locus_tag="PC621_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF4110730.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
BASE COUNT         1287 a          481 c          466 g         1294 t
ORIGIN      
        1 tatttatgta agaaataaat tttatataat attttttcac taaatttttt tctttttaca
       61 acaaatactc actacctttt gatataatat ttttatcaat tattattata ttcaagtttt
      121 gaaaggagta ttaaaatgag tttattagtt ttcggacata aaaatccaga tacagattca
      181 atatgttctg caatttcatt agcacattta aagaacaaac taggaataaa agcaacagct
      241 tatgctttag gagatatcag aaaagaagct aaatatgcct tggattactt taaggtagat
      301 gcacctgaaa ttttagataa tgttagaata caagttagag atttaaatta tgataaagta
      361 gtacctttaa caccaacttc atctatatta gaggcttata accttatgga tgaaaaaaat
      421 gttaaaacac tacctgtaac ttatgatgat ggaacttttg ctggtatcat tactatgaaa
      481 gaaatagcta aaaatctact tcatcaacac tttacaacta tacacacatc actatcaaac
      541 atttgtaaaa accttaatgg tactattctt gtggactgtg gtgaagatat aaaaggtaga
      601 gtagtcactt tatcatttgg aatggagact ctaatagaga ctttaaaaga aggagacata
      661 gctattgttg gagatagata tgatagtatt gaatatgcta ttgatacaaa agttaaactt
      721 ttaatactta ctaatcatac agagatttca aaagatttgc tagctcttgc taagataaat
      781 ggagtgtctg ttgtttctgt tgaaagtgac aattataaaa cttctaatgt aataaatcaa
      841 tgtagtttct tagactctat agaagctaca gaaaatttaa ttacattcaa agaagatgat
      901 tatatggatg aaataaaaga gattatgcta gaaacaaact tcagatctta tccaatatta
      961 gatgatgata ataagttttt aggtttagtt tcaaaaggtc atctattaaa cccatctaaa
     1021 aagaatgttg ttttagttga ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa
     1081 gcaaacatag ttgaaataat tgaccatcat aaacttggtg gtatttctac tgatgttcct
     1141 atgtcattta gagttatgcc tgttggatgc aatagtacta taatttatca aatgtacaaa
     1201 gaaaacaatg ttgagatacc ttatgaaata gctgggcttc ttttatctgc tatattatca
     1261 gatacattat tatttaagtc tccaacaaca actgatatgg ataagaaagc ttgtgaagaa
     1321 ttaagtaaaa tagcaaaagt agacatggaa aaatacgcta tggatatgtt taaatgcggt
     1381 acttctttag atgaatatac tatagaagaa atagtaaata tggattttaa agaatttaat
     1441 atgagtggac atagagttgg tattggacaa gtatttacct tagacataga ctctatattt
     1501 gctaaaaaag atgagttttt atcttatata aattcgactg attatgacaa actaattctt
     1561 gcggttacag atataatcaa agaaggttct tatttaatct ataaagcaga agatagctta
     1621 attgctaacg catttgatgt tgaaggtgtt caaggtacat ttgcaccagg tgttgtatca
     1681 agaaaaaaac aattagtccc taatttaact actgcaatca aaaattttaa ataatataaa
     1741 atttttaaat aacgaaaact gtctcaaatc tatatttgag acagtttttt attatacata
     1801 tattgataat aaagttaaat aaaaatccat aaatttaaaa tttttatgaa tatattttat
     1861 tattattgtt attttattca ttctaaaata ggtaaaactc aatatataaa aatacttttg
     1921 tcatttaaat aaaacctaaa tagtttttaa gattatttta gacacgtttt aagtcataac
     1981 agtatcagct attctaaata tcgtcaaagt agccgcagtt cctgctgatt gcccatttcg
     2041 tatccctact gttgtagatg aaaaattatt tattgtaaaa gtagtccccc caaataaaaa
     2101 accagcaaaa tatccagaaa gtactcctcc gcctcctggc tcagtagtaa gccctgccaa
     2161 atcttttcct cctaaaattg ggaataggat agcaaaagaa tattgattta tacttgatga
     2221 acctggttgt aatgctactg aaactgatat ataatacaca ccattttctt gtactgtaaa
     2281 cgtaccattt gcatctactg ttatacttga actatttcct attattgttt accaacctgt
     2341 tatagcattt ccattattta gtgtttgtgg agatggtgca tttacgagga agttagcaat
     2401 tggctgagag gaattgcctg ttgctcctgt tggacctgtt gctcccattg gtcctatact
     2461 tcctgtcgct cctcttggtc ccattggacc tgtaattcta attggaagac actcacattt
     2521 aggataacaa ttatctataa ttctatcttc aaatgttaca agtatatacg gtttgttaca
     2581 atccctacta gatgtaaatt caaaaatctg ataagggtaa tttaaagata aagctaaccc
     2641 ataattagga tatttattca ttgcccatgc ttttatagtt cccgtaatat ttaaacaaat
     2701 atagttggat gtatcactta tcccaattgg taaatatccc ctgatatttt ccatagacac
     2761 tctatcattc catgttatag ttcgtaaatt ataatattca cttattcttt taatttcaat
     2821 atctactttt tctttaaaaa tatgattctt atttgaatca atatgaataa ataactctgc
     2881 ttttaatata cttttgtctt ttaatatatc tggtattctc acttaaaaaa aagtaatcaa
     2941 cttttctttt acaaaactat ttgaacattt acttcctatt aagcaataat ctaaattact
     3001 aaagttttta tctggatatt ttttagatat aaaagtatca tcatttaaat aaagtataat
     3061 ttttctcatt tataatccct ccattttggg tctatatcat actatgttta aaatacatgc
     3121 ttagttacaa tacaattaaa atttatagta ttaaattttt atataataat gctgatttct
     3181 acaaaaaaag aacatgtcat tagaaacttc taatagcatg ttctttttca ttattatata
     3241 ttacacttta aattattcct tgactggctt agctggtgta aactaaacca gtctttctaa
     3301 aatattaagc acttttttct tattatgttt tttaggattt attgtctcta ttattgtaaa
     3361 ccaaagttat aaaattgtaa atagaatata ttaccataac aatatttttt tataagtaaa
     3421 aattataaaa aaccttattt ttattaaata gaataaataa aataagttta aaaaatactg
     3481 aaataagtgt tttccacata ttttatatat ttatttaata cattatta
//
LOCUS       DAMKPG010000189         3308 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMKPG010000189 DAMKPG010000000
VERSION     DAMKPG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.196"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            160..1413
                     /locus_tag="PC621_003623"
     CDS             160..1413
                     /locus_tag="PC621_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF4110731.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
     gene            1747..2478
                     /locus_tag="PC621_003624"
     CDS             1747..2478
                     /locus_tag="PC621_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110732.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            2720..>3308
                     /locus_tag="PC621_003625"
     CDS             2720..>3308
                     /locus_tag="PC621_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDF4110733.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         1407 a          319 c          494 g         1088 t
ORIGIN      
        1 gttatatata taaataatac tagaaaggga ggattatatt atgatattta gtaaacgctc
       61 ttttagaaaa aaacggtgat aaacttgttt taaattttag tagaatatga tttttgtttt
      121 ggcaagtatt aatacatata ttaaagaggg aggtttaata tgttaaataa aaaaaatata
      181 agttcaatta ttatgacaat gattttaagc ttatcaacta ctgtatttgt tttcgctgat
      241 gaaaatacta atgtgaaagg acaagatgta accaaatata atgaagaaat ggtctctaaa
      301 aacttagaag atttaagtat agaacaatta atagatatta gagataaagc tattgataaa
      361 aatttagaag gtcttagaaa atttggagaa gattcagtga aagaacgtaa agattcaatt
      421 ttaaactttg aatttggatt agtagctttt gttaacaaaa attatagtaa cttaacagaa
      481 aaagagaaat taataagaca aatcaatatt gttgagtatg aaaattctat aaatgatata
      541 atggatcctt caggcgattt tttcaaagta acaaaccaaa gttatcagat taatgaattt
      601 gtattaaaaa atggaaaaaa ctataaagag tggtcgtatg aatatgtaga gtttttgatt
      661 gagcatttag aggatttatg taattttgta gattttgatg caaaatatgt aatagaaatg
      721 gttgatcctt caataaaacc agatctttca gataatcaaa agaaatctct aaataatgaa
      781 ttaataaaaa tgtattcaag taatactcta agtacaaata taaaagatga aatagaaaac
      841 tgggaaaata atttaaatat taatgagaaa aattcaaatg aacctttacc aagagtaagc
      901 ccttctagtt atagacaaca agcattagct tatgctaaaa aacatggcta tactaatggt
      961 tattatggta gtacaaatag aaaaaataat gcaccttctc catattataa ttttcaaaaa
     1021 gatggatatg gagattgtgc taactttgtt tcacaatgct tacatgaagc tggagtaaca
     1081 ttctggacga ataataatcc atggtattat tattctacta gtaatagatc accttcttgg
     1141 gctggagcaa gagaatttaa aatacattgg atgaatcgaa ttgcatatca aacacttact
     1201 gtatcaaact ctttaagttt tttaagacca gcaacacctg taagtatatt aaatagtagt
     1261 ggagtagcaa ctcatacact aatatctaca gataagcatt caaatggata taatttttca
     1321 tatgctgcac attcagatga aggtgcaaga acaaatttgt taaaaaaact tgaaggaaag
     1381 aaaatttctt attacaaagt ttattgggaa taaataaaaa tttatacttt taattaaatg
     1441 aaaattgaaa agaataaata tctatttcaa gatccaataa gtatttagtt gaagcagcta
     1501 ttaaatttta ttacataatc ataaaaaaga gactagtgga atttagtctc ttttttatga
     1561 tttaaaaatt taataaaaga attatataaa tttgtctaaa aattagttta tcattcgtta
     1621 taattatata aactatatca aaaaagtgtt ctattgtatt ctttagaata atcagtaata
     1681 aaggttattc taataaactg agttattttt tcacaaataa aatacttatt aaaaaggaga
     1741 tttgatatgt taaacaaaaa aaatgtaagt ttaattgttt tagtaatagt tttaagttta
     1801 ttcactgcta tatttgtttt caataataga aatacaaatg ataaatcaaa agaaattgct
     1861 aaaaataatg aagaaatagt gcctaaaaac ttagaagctt taagtataga acaattaaca
     1921 aaaattagag ataaggctat tgataaaaat ttagaaggtc ttagagaatt tggagaagat
     1981 tcagtaaaac agcgtaaaga ttcaatttta aattttgaat ttggattagt tgcttttgtt
     2041 aacaaaaatt atagtaactt aacagaaaaa gagaaattaa taagacaaat taatattgtt
     2101 aactatgaaa actctataaa tgacataatg gatccttcag gttcattctt tgaattagca
     2161 aatcaaagtt atgaaattaa tgagttcatg ttaaaaaaca aaaaaaatta taaagaatgg
     2221 tcatatgaat atatagaatt cttaattgat catttagatg aattatgcaa atttgttgat
     2281 tttgatataa aagatgtaat agatattatt gatcctacaa taaaaacaga tctttcagaa
     2341 gaacaaaaga aatctttgaa tgataaatta aagaaagtgt cttcaagtga tactttaagt
     2401 gaagacatta agaaggagat aaaaaattgg gaaaacaatt tgaatagttt aaatatgaat
     2461 aaaaataatt ctaattaact gatagtgtaa aatgtacata attataaata aaatagggat
     2521 gtcttaaaat tgattatttt ctaatctaaa tattttaggc ctatctaaaa acataagatt
     2581 atggtctaaa atttgaaatt ttagaccatg ctttatcttt acaataaatg ggtctttaaa
     2641 ataaagtgtg tattcttcta aaaagtggtg ataattaaaa tattaaatat cactatataa
     2701 aggaaggtgc acacttttta tgtttaacaa aaacgaaata ttaaaaaaac ttattgaaga
     2761 atatgatgtt aaaacaacta cagatattca agatatgcta aaagatttat ttgcgagtac
     2821 aattcatcaa atgttagaag cggagcttga cgaccattta ggttatgata gatatggcaa
     2881 taagaataaa aatactaaaa actctagaaa tggatataga aataaaaatg ttaaatcaga
     2941 ttttggagaa ataactttaa atattccaag agaccgaaat ggagattttg aaccaaaggt
     3001 aattagtaat tataaaaatg atatatcagg aatagagaac caagtgattg gaatgtactc
     3061 aaaaggcatg tctactcgag atatatcaag ccaccttaag agtatttacg gaattgatat
     3121 atctcattct ctagtagcta aaattactga tagagttcta cctttagctc atgaatggca
     3181 aaacagacct cttgatatgg tttatccaat tgtatttatg gatgctatac attataaagt
     3241 tagaagtgaa ggtagaataa taaacaaagc tgcatatata gccataggtg ttaacttaga
     3301 aggtataa
//
LOCUS       DAMKPG010000190         3306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMKPG010000190 DAMKPG010000000
VERSION     DAMKPG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.197"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            111..722
                     /locus_tag="PC621_003626"
     CDS             111..722
                     /locus_tag="PC621_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF4110734.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            1020..1358
                     /locus_tag="PC621_003627"
     CDS             1020..1358
                     /locus_tag="PC621_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF4110735.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1765..3201)
                     /gene="miaB"
                     /locus_tag="PC621_003628"
     CDS             complement(1765..3201)
                     /gene="miaB"
                     /locus_tag="PC621_003628"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF4110736.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1147 a          480 c          372 g         1307 t
ORIGIN      
        1 tataataata tagatagtta ctatatctta taattagata aatcataatt atatattatt
       61 gttaaaactc aattatataa tttttaataa tattatttta ggaggtaaaa atgataaacg
      121 atattaaaaa taaattcaaa aattacaaac catatataaa tggatacaag aatatgaaga
      181 gagcatctgt tttaattcca ataatagaaa taaataacac acactatata ttatttgaag
      241 ttcgctcaaa aaatttgaag catcaaccta gcgaaatttc ttttccaggt ggtaaaatag
      301 aatcaggtga gactccatat gaagctgtta ttagagaaac ttgtgaggaa cttggaactt
      361 ttagtgacaa tatagaagta atatcaagtc ttgacctcct tataacacca gtaaagttta
      421 tcatacatcc atacttagga tatataaaaa atattaataa tttaaatata aacaaggatg
      481 aagttgacca tacttttttg gttcctataa aatatctttt agagaatcct ccaaatactt
      541 atagtaatac tgtaaaagtt tttccagatg aaaatctacc atatggttca attccaaacc
      601 aaaaagatta taaatttgaa agtggaagtt atcctattct gttttataaa tataaagatt
      661 atgtaatttg gggaatgaca gcaaaaatac tagaaaactt tttaaatttt ttatcagaat
      721 aacactcata ataaatcatc aaaactttta cataagaaat ttataaaagt ttctaaattt
      781 aacaattcta aaatatcaat aactatattt tgtagcactt ttttatttta ttattgtttg
      841 aatatttgat ggttttcaat gcaatttcaa cataaatact atgtattagt gtatactgtt
      901 gatgaattaa agtatgataa tctatttaat aaagtatgaa gtaaatgatt ttaatgtgtc
      961 tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag gtgcttataa
     1021 tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta agtgtactat
     1081 tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt aagaaagaaa
     1141 aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact tttattgtct
     1201 ctataatgct atttattatg gcatttttgg gatatagaat agagagtggt caaatggacg
     1261 aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc agtgtatttt
     1321 ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt tgagcagtta
     1381 attataaaag attgactgct ttttcatgta tattgactac tcttattaaa tattttaaca
     1441 tttaaaaatc tatgtaaaaa taatgaatat agtacaaact tctaataatg attttacctt
     1501 tgtggttaca gtccactata tataatttaa tatgataaca tggaattact aatgacaact
     1561 aagtaagctt ttataaaata taataaaaca aaaatagatg tacaatgaaa ttatatctta
     1621 tatcaaattt atttttatta atagcaaaaa cttacattag cataacatat gacaaattcg
     1681 cttaaaataa aaatttttaa tataatttgt tctatagtac atctatttcg attagtttac
     1741 ataattttat tattttgtac aatattaatt atttactaat atcccattta atgaaaaagt
     1801 tctaggttca gtaattttga ccataactaa tttaccaatt aaatcatcat ttccaccttc
     1861 gaaatttacg agtttatttt gtctagttct accagcaaac ttattttcat tatttttact
     1921 tcttccttca actagaactt caactattct atctttatat ccatcattta tttctgcaga
     1981 aatcctattt acagcttcta acaccctatt aaatctatca tgctttatat cttctggtat
     2041 ttgattttcc attttagcag caggagttcc ctgtctttta gaataaataa atgtaaaagc
     2101 tgaatcatat ctaacttttt caaccacatc taaagtgtcc aataaatctt cttctgtttc
     2161 tcctgggaaa ccaatcatta agtctgtaga aaaagcaata cctggaactt cctttttagc
     2221 tttttctatt atttctaaat aatattcctt tgtataatgt ctattcattt ttttcaaaag
     2281 gctactgctt ccacattgta ttggtagatg taaaaactca cacaccttat cacaatctct
     2341 aattgcgtat attacttcat cagatatatc ttttggatga gatgtcataa atcgaaccct
     2401 ttcaattcct tctatatcat tcacttttct taaaagttct gagaatgaaa ctggattttc
     2461 aagtgtctta ccataagaat ctacattttg tccaagtaaa gtgatttctt tcgttccatt
     2521 tgcaacaagc tcttttatct cgttaattat atcttcagga gttctacttc tttctcttcc
     2581 tcttgtgtat gggactatac aatatgtgca gaaattattg catccataca ttatatttac
     2641 aaatgctttt aattcaaatt ttctattact tctaagacct tctattactt ctccatccac
     2701 atcccataca tccactaaca ttttgtcaga atttattgat tctgtaagta attgcggaaa
     2761 tttatataga ttatgtgttc caaatataag gtctacatgt ttatattttt ttcttaattc
     2821 ctctacaaca tgtggttgtt gcatcataca tccacataca gctattttca tatctggatt
     2881 ttttcctttt agatgtttta attgtcctaa gttaccatac acttttagct ctgcattttc
     2941 tctaactgca caggtgttat atattattaa atcactttct tcaaccatca ttgacatttg
     3001 gtatcccatc tcttctaaca tcgaacataa tttttctgaa tcatgttcat tcatttgaca
     3061 cccaaaggtt tgagtaaaca cgagttttct ctttccagtt ctgttgaagt attcttcatt
     3121 ttgtctgtgt atttgttcta tatatttatc ttgttcttgt attttctcta taggtattgt
     3181 tacttgtttt cttttgctca cgtctattcc tcctagttaa tttcttatat tacacataac
     3241 cttaattata tcaaataaga aaaatctttt aaagatatta ttatttttct agttgtgttt
     3301 taatag
//
LOCUS       DAMKPG010000191         3303 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMKPG010000191 DAMKPG010000000
VERSION     DAMKPG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.198"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     misc_binding    169..379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            605..1420
                     /locus_tag="PC621_003629"
     CDS             605..1420
                     /locus_tag="PC621_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF4110737.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1448..2125
                     /locus_tag="PC621_003630"
     CDS             1448..2125
                     /locus_tag="PC621_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF4110738.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2184..2906
                     /locus_tag="PC621_003631"
     CDS             2184..2906
                     /locus_tag="PC621_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF4110739.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1330 a          333 c          607 g         1033 t
ORIGIN      
        1 aagaaatagc taaaaatatg aaatatacta tttataatta caaaaataat atcaataaat
       61 ataaacagta gtatttagat aatttacaag taaaaattat taaaaaaata aaaaacttat
      121 tgacaaaaat attataagga agtacaattg tattaacaat ttaaggaaac aaatccaatg
      181 atagagaaag tagcatatat ggatttacag cgagttaggg gttggtgtaa gcctagcaat
      241 gaagtctatg tgaagagagc tctggaggag attatcagaa actttagtat gataattggt
      301 ttacccgcct taagggattg agaggataag tacttactta tcaaaaagag tggtaacgcg
      361 gatataattc gtctcttagc tgtaaagcta agggactttt ttgatttaaa aataaaaata
      421 tccatacagc ttaaatgcta acagtcacaa attttgaaag taatataagt atttatcaat
      481 aagagtggta acgcggtaaa gtcgtctctt agtcattaaa gactgagaga ctttttttgt
      541 aactcgaaac atttaagtat taaaatctga gggaaattat aattaaaaga gaggggtagt
      601 taaaatgaaa gtatttaaaa aattattaag tttaggatta gtattaggat tgacattatc
      661 gctagtagga tgtagtggtg gaggagaaaa aacaaagtta gaacaaataa aagacaatgg
      721 aaaattagtt gttggtacta gtgcagaatt tcctccattt gaatttcata aggtagtaga
      781 tggaaaagat tcaataaaag gattcgatat aatgttggct gaagaatttg caaaagagct
      841 tggtgtaaag gttgaaatta aggatatgtc atttgatgga ttaataggtg ctttaaatgc
      901 agaccaagtt gatattgttc ttgcaggtat gtctccaaca ccagagagag aaaagagtgt
      961 tgatttttca gaattatatt atttaagtag aaatgcagtt atagttaaag atgcagatat
     1021 agacaaagta aaaactgaag atgatttaaa gaaacttaga gttggagttc aagcaggaag
     1081 tatccaagaa gaatatgtag ttaacacttt aaaaatgaca actacaaaat ctttaaaagc
     1141 aatacctgat ttaattacag aattaaaaaa tggaaacata gatgcagttg ttactaatga
     1201 agcagtttct ttaataaatg ttaaaaaata tgatggaata aaaatggcaa atactgaagt
     1261 tggtaaagat gtaacagaag gtatggcagc agcaatcaaa aaatctgaca ataacaaaga
     1321 ttttatagaa ttattaaaca aaaagataaa agaattacaa gatggtaaga agatagaaga
     1381 atttttaaat gaagcatcta ctgaagctgc ttcaaattag taagaaagtt atgtgggaga
     1441 tgaaattatg agtttagatt ttagtttttt aagtagattt gggacttctt ttttggaagg
     1501 aacaggtgtg acagtatcaa tttctcttgt ggcattatgc tttggattta taataggtat
     1561 aattatctgt atggcaaaaa tatcaaagag caaagtatta agagcaatat catcaatcta
     1621 tattgaggtt ttaagaggga caccattatt agtacagata tatatagtat ggtttggttt
     1681 acctcaatta ggaataagat tccctatgtt gtttggtata ccaagtgaat ttatagctag
     1741 tgcatttgca ttatcagtaa actctggtgc atacgtggca gagatactta gatcaggtat
     1801 acaatcagtt gataatggac aaatggaagc aagtagatca ttaggattaa attattggtc
     1861 aactatgaga tatataataa tacctcaagc tataaaaaat atattacctt cattagcaaa
     1921 tgagtttatt acacttgtaa aagaatcttc aataatatct gtaataggag ttgttgagat
     1981 aatgcgtact gctgatatag taaaaaatgc agcatttaga gcattagagc cattaatagt
     2041 agcagcagca atttactttg ttataacatt tactctatca agattagttg gattattaga
     2101 aaagaaacta tctgtgtcta actagatgag agcatgttta gcatttaggg aaaacataga
     2161 atagtaaaaa ggaaagtgat aatatgatta caataaaaaa tttaagtaaa tcttttggag
     2221 atttaaatgt attaaaaaat atagacttag aaatcgctaa aggcgaaata atggttatag
     2281 taggtccaag tggttctggt aaaagtacac ttcttagatg tatgaattta ctagaaatac
     2341 ctacaggtgg agaaataata tttgaaggaa aaaatttggt cgataaaaaa actaatattg
     2401 atgaggttag acaaaatata ggtatggtgt ttcaaaactt taatttattc ccacataaga
     2461 ctattttaga taatataaca ttagcaccaa ttaaattaaa aaagatgact aaggaagaag
     2521 cagaaaaaaa agcagaaatt ttgttgagta gagttggtct tttagataaa aaagattcat
     2581 atccatcaca attatctgga ggacaaaagc aaagaatagc aatagctaga gcacttgcta
     2641 tggaaccaga tatgatgttg tttgatgagc caacatctgc tcttgaccca gaaatggtaa
     2701 acgaagtttt ggatgttata aaagaacttg ctaaagaagg tatgacaatg gcaattgtaa
     2761 ctcatgagat gggatttgct aaggaagttg cagatagagt tatttttata gatggaggaa
     2821 gtatacttga agataatact ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa
     2881 aagcattttt agcaaaagtg ctataatata taaaaaggat gttattgata ttagaaattc
     2941 aatacatcct tttttagtga tggttaatat taattttaaa attaaagtta tattaatatt
     3001 aactttattt ttacgatttt ttgcagaaaa aaaaagagct cggggtaagc tctttttttc
     3061 caccgaatta atttccgtta ctaatccgat ttttaataaa tataaaatat atccttatga
     3121 aagggtattg ggaatagata tattaactaa ctagggttac cagggggata accttaattt
     3181 attataacaa aatgcgacac aaaaatcaat attttttttg aaataaatta taaaagttat
     3241 ttttataaca agtaagctta taaatttaac ttttatagta aaaataagta tatgtataat
     3301 ata
//
LOCUS       DAMKPG010000192         3242 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMKPG010000192 DAMKPG010000000
VERSION     DAMKPG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.199"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     repeat_region   67..360
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=134..162
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1021..3114)
                     /locus_tag="PC621_003632"
     CDS             complement(1021..3114)
                     /locus_tag="PC621_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF4110740.1"
                     /translation="MNKRNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEQNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTILNKPIKINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1137 a          517 c          308 g         1280 t
ORIGIN      
        1 ttatattttt gtatgctaat ataactagat aatcataaga taacacatta aaagaaaatt
       61 ataaaaattt atattccgta tagttagtat aaaacgtaag taatataaat ttatgtaaaa
      121 caacaaaaga tggatttaca ttccatatag ttaatataaa acgttttaat gaattaacag
      181 aagatgaaag ttttatatgt atttacattc catatagtta atataaaacc attgaaaata
      241 gtgactttta cgaaaaattt gctaaaattt acattccata tagttaatat aaaacacttc
      301 ctattaaatc cacattagga aattctccga gatttacatt ccatatagtt aatataaaac
      361 cccaaaataa acttagtatt ttcaacactt acacgtatac aactctccta aatttgcagt
      421 gaacgagcaa tagtgcaatt gataacgctt atcatgcact ctcaacacct tatatttcaa
      481 ctgttacgcc aaattttatc acaaatatag ctcactgcaa aatttctata attttattat
      541 atcataaata tattactttt gaatattgat accaatttat accataataa aagcaagaaa
      601 aaacgtaatc tatctacatt ggatttaaat tctataactt aaagtttttt acataatatt
      661 tatgcttgtt taatcaatct aatatatcat tcttgtatat tgcttttaat agtctttttt
      721 cttaaataaa tatctatctg aacaagcata gtttaattaa aaacttttgt attcccttaa
      781 aactgtatct acctcttatt attttattta tgatatatta tttttaatct aaaaatagat
      841 atctattttt attatagtta tcccttttta aaatacttta atcatatact actcctaatt
      901 taacatccct tgaaatgcta ttttcttata ttgcaattta taattgatat atcttcaata
      961 taataattta atgtattttt gattttaaag cttttcagtg atattattga taaattagaa
     1021 ttaattatct agctttaaaa ggtcaaagat ttctgttgct gtctgcggtt ttccaaataa
     1081 atatccttgt atgtaatctg gctttatttg tttaactaaa tcatactctt cttgtgtttc
     1141 tacaccttct aaacatactt ttattcctac atcatgacaa attgcaacaa taaaatgtat
     1201 aaatgtagca tcaaacttac tctttaatat atctttaaca aatactctat caattttcac
     1261 tatatctatt ggagaaaatt ttaaaacttc tagagacgag tatccagtac caaaatcatc
     1321 catagcaata taaattccta aatcttgtaa agccttaaat ttaaactgta aaatactcat
     1381 attttgtact gtatgacttt ctgtcaattc taaaacaaga ttttgataag gaaaattttc
     1441 atcatctata atttttacta tatcttcaat aaatgtgtct tctaatattt gaacagctga
     1501 aacgtttaca ctcactttaa aaaaaggata gtaatcaatc catttcctaa aagtacgtaa
     1561 tgccattcga agcacccaag cccctacaat atttatcata tcattttctt ctaatattgg
     1621 aataaattcc aaaggagaaa ttgctttgca tttgtcatta gtccacctta aaagaacttc
     1681 tacacctatt attttatgac tttgtgtatc tacttgtggc tggaatcgaa gactaaatcc
     1741 cctaaaatca tcattaattg atgcctttaa atcccgcatc atttctaatg aatacatttt
     1801 attttttaat atttcttggg agaaaaaaac aagcctgttt ttaccatgct ctttcgcata
     1861 ttgtaaagag taactagcgc atttataaag ttcttttact gtatctccat gttttggata
     1921 tattacacaa ccagcagaaa ttgttatatt taatccatat tttctccata gttgaaggtg
     1981 tataatcata ttctgtattt gattatatag agttagaatt tcattttctt caacattatc
     2041 aacaagaata cccatttcgt ccccatcaag tttataaagt tcagcattat ctggtaaaat
     2101 agattgtata gactgtgcta gtgtttttat tatattatct ccaaaatccc tatcatacat
     2161 ctcattaaca tttttaaaat cgtcaatatt taataatact atgcataatt tttcaatcat
     2221 aggatttgaa attttatctt caaatacact cataaattca tgataattca gtaactgtgt
     2281 caatgaatca atcttatttt gtttacctaa ttgcgtcatt attcctgcaa aaatacttgg
     2341 ttctccaaat tcatcacgca ttagttgtcc tctacatctt aaccatatat attctccact
     2401 acgattttta gccctaaatt ccactgtatg ataatccatc tggtttttac ctatttcagt
     2461 gtttgactta taaaaacgat tccagtcttc tggatgtaca atttttttcc agtaaataag
     2521 cggattttca acaatttctc ctggtaagtc aaataattca acttgtgatg gtgaatatcg
     2581 aaatacgcct gttttcatat tacaaatgta tatatattca tctgtacctc taatcaaagc
     2641 atcataaagc aatgtagaat tataatcaaa attgtctctt gaaaaattaa aaactccttt
     2701 tccttcttta gtatttgata tttcattaaa ttcattaatc ttaattggtt tatttaagat
     2761 tgttgagttt gcaatatcta ttgattgatg catatgaaca actgtgccat cttgccatgt
     2821 aattaaacta tcataatttt caaaaacacg atttaactta ttacattttt cataccactt
     2881 tattaatact ccttttttat cattctttaa tagttctaaa actttacaaa aactacaagt
     2941 agaattttgc tcaggatata atacttgcca gcaaactttt ccttctggat ctaaaatatt
     3001 atattcttct ttcattttct tgttcataaa aataatttca ttagtatgaa tatttgtaac
     3061 atatatatta atctgtagtt gatttaatat aacttcaaaa ttacgtttgt tcatatactt
     3121 atctcctcca tagttactgc tctttataac aacactgtta aaattattct ttttagaaga
     3181 tttacttata acttatacat acacactaaa taccttaatt taattagaaa gtttattatt
     3241 tc
//
LOCUS       DAMKPG010000193         3224 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMKPG010000193 DAMKPG010000000
VERSION     DAMKPG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.200"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(332..589)
                     /locus_tag="PC621_003633"
     CDS             complement(332..589)
                     /locus_tag="PC621_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF4110741.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1169..1390
                     /locus_tag="PC621_003634"
     CDS             1169..1390
                     /locus_tag="PC621_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF4110742.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2082..2312
                     /locus_tag="PC621_003635"
     CDS             2082..2312
                     /locus_tag="PC621_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF4110743.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2325..2474
                     /locus_tag="PC621_003636"
     CDS             2325..2474
                     /locus_tag="PC621_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF4110744.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2731..3168
                     /locus_tag="PC621_003637"
     CDS             2731..3168
                     /locus_tag="PC621_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF4110745.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1293 a          410 c          339 g         1182 t
ORIGIN      
        1 actaatccct taaaatatct atttatattc ttaattttat aaatatatta tgtcatatta
       61 attgctattt ctttatttat atagtttatt tatttatttt tcccctttat acaagcaatt
      121 gtatcatttt atctattttt tagccaacaa atattttcga ccattttctt tgtattctca
      181 atatatctcc agtatttaac aaattttttt atagaatata caatgtattt aaattaataa
      241 gtagtaaatt tagttaattt gtctaaaaaa actttataaa acatcttctt tgtctttact
      301 cttacattta acaaggctat tttcattatg tttaaacatt attttcattg agtattcgct
      361 taatattgct tacacacata ttatatctaa tagccaattc attatggttg actccattaa
      421 attgttttac tatatcccta tttctagcat atctaaaaag ccccttatac gtaggtatat
      481 aagtatttgt tcctccatac aacttggcaa cctctatgaa cttatcaatt ccgattattt
      541 ctaccattgc atgaaattga actggtatat cctccacttt tatcttcata agttacacct
      601 cctttaagat atttttatag acttaacaaa gtttttattt aatacattat tgactaaact
      661 atatttatta gttttattga atctaataaa aaatttctta gctttaaaat taatttttgt
      721 attaacttta ataaaatttc tcataactta taactttgtt agctttaata aagattttaa
      781 tggtttaaaa gtaaaatgtt aatattggac ttttaacttt tttatttaca gattaaattt
      841 aataaaattt ttaacataag ctttaacatt taatttaaca taaactttgg tacctaacat
      901 ttaactctac acatatgaaa atccatacaa aactatctca actaagcatg aatatataca
      961 aatactattt caaaacacta agaacatatc ttcaaaaata acttgtaaac tttttcatca
     1021 tcagaaaagt aaaatttttc ctataaaaat ttacacaaaa aatctttaga aattactgtt
     1081 taactccgat attatcttag atgatagtat tatagctaac aaagaaagtc gacaaatctt
     1141 tgaaattaaa tttttaggag gtaattttat ggaagttgtt tcaacaaaaa atttatctag
     1201 tataaaacta aaattggatg ctggttttga tgataagggt aaggcagttg ttaaaagtaa
     1261 atcatttgca aatgtaaaag cagaggcatt aaatgaagat gtttatgctg ttgcagaagc
     1321 aatagcaagt cttcaagaga accctattgt agatatttta aaattagata gtacatcatt
     1381 atctaaatag tatcttttaa acgtactctg ctttacaaat taaaaaaatt aactctttat
     1441 ataaaagatt aagttatata acgcacttaa tacgctatag atttggcaga ttataacttc
     1501 aattttggtt ataactttaa tatattaatg ctttgaaaaa ttaatatatt aaacgtattt
     1561 caacttaaac atgttagcta ttttaaatgc taccctactt taatctaatt aattgaaata
     1621 aattaaatat tttctatatc agcaataact agagtcaaca attaaaatac taatattgac
     1681 aattgaaaca ctgatactaa caaacaaaac actaatattg acaattgaaa cactgatact
     1741 aacaaacaaa acactaatat tgacaattga aatactaata ctaacaagca aaacactaat
     1801 attgacaatt gaaatactaa tactaacaag caaaacacta atattgacaa ttgaaatatt
     1861 aatactaaca agcaaaacac taatattgac aattgaaata ttaatactaa caagcaaaac
     1921 actaatattg acaattgaaa tactaatact aacaagtaaa acattaatat tgacaattga
     1981 aatattaata ctaacaagca aaacactaat attgacaatt aaaataccaa tattaacaag
     2041 caaacaatta aaatataaat tattatatcg gaggtaatta tatggaaatt aagaaaaaat
     2101 tagtcatgag ctttaaaaat gtaaaggata aacaagtttc tttttctata gaaaatccaa
     2161 aggatgattt aactgaagaa aatataaaat ctgtcatgga tttgatagta tctaaaaata
     2221 ttttttcggt tggaggattt gacctagctt ctgttgttga agccaaggtt gtggaaacaa
     2281 ataccactcc ttatgactta gttatagaat agggggctaa ttttatggat gatagtttac
     2341 aactgttaat aaccaatgtt ggttttccta tagctctatc catgtacttg ttggttagaa
     2401 ttgagggtaa attaaatact ttatcaaaca gtattgatac attatccaaa aatattttga
     2461 tatttaagaa gtaatttgac aatatatgta agtcataaaa taggtctatt tgtaaaataa
     2521 ttagacatat tctatgctaa atactaggat atttggataa tttatccaaa tatccatagt
     2581 atttttttga attttaccac ctcaaatttg aatttcacta ttttattttt gaatttcact
     2641 atgcttaatt tgaaatttag aacgcatcta gttggactca ataagatata atgctattct
     2701 aatgacagta ataaaatgag gtgatgatta atgaaaatac atgtcgaaca aggtattaat
     2761 tgtgatgata ttgaaataac cataaaatgt tctgctctag ataataacat tgaaaaaata
     2821 atttcattat tgagtacacc attacttgat ataaaaggta aaaaatcagg agagatttat
     2881 aaactcaatg tctgtgatat tttatacttt gaaacagttg gaaataaaac atttgcttat
     2941 tatgaagatg atgtatttga aattgattta aaaatatata aattatcaaa attattagct
     3001 aatacatcct tctcacgaat atctaaatcc atgattttaa atatggacta cataaaaagt
     3061 ataaaagtaa tatcacataa tcgcatgcaa gctactttaa aaaacaacga aaaagttatt
     3121 ataaatcgtc gttatataaa aaatattaaa gaaaagctaa acatttaata aaaatctaaa
     3181 gtaatttatt aatatcacta attgcaaatt tattgaccta ttta
//
LOCUS       DAMKPG010000194         3083 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMKPG010000194 DAMKPG010000000
VERSION     DAMKPG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.201"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(145..666)
                     /locus_tag="PC621_003638"
     CDS             complement(145..666)
                     /locus_tag="PC621_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF4110746.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="PC621_003639"
     CDS             794..2158
                     /locus_tag="PC621_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF4110747.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="PC621_003640"
     CDS             2347..3024
                     /locus_tag="PC621_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF4110748.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT          964 a          440 c          499 g         1180 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caa
//
LOCUS       DAMKPG010000195         3038 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMKPG010000195 DAMKPG010000000
VERSION     DAMKPG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.202"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            99..605
                     /locus_tag="PC621_003641"
     CDS             99..605
                     /locus_tag="PC621_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF4110749.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            733..1749
                     /locus_tag="PC621_003642"
     CDS             733..1749
                     /locus_tag="PC621_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF4110750.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2103..2807
                     /locus_tag="PC621_003643"
     CDS             2103..2807
                     /locus_tag="PC621_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF4110751.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1282 a          284 c          526 g          946 t
ORIGIN      
        1 tgtaaaatct tcctatatca tataaaatta agtattgatt agtctattac ataaatatag
       61 aatataaata tatccattta ataataagga gaagagatat gaaagaatat gtagttgtgg
      121 atttagaaac tacaggatta gatccatata aaggttgtga aataatagaa ataggaataa
      181 cagaaataaa aaatgaacaa attgtaaaaa actattcaag gttgataaaa cctaagggga
      241 taatttcttc atttataaca gaattgacac atataagtaa tgaaatggta gaaaatgaag
      301 aaccactaga attagtttta cctagattta gaaaatacat aggtgataga actataattg
      361 cacacaatgc gaaatttgat ttaaaatttt taaattacta tcttaggatg ttagatttag
      421 aaccaataaa caattatata tgtacagtag aattattaaa aaaatgtaaa agttataaag
      481 gaaaaaacaa gaaacttgaa acagcttgtg cttattacaa catagagaat ataaatgctc
      541 atagagctga cagtgataca ttagcaactg caaaactatt tttgaaaata aaagatgaat
      601 attaattata aatatagaga aaattagtga aaatatcctt tataaaattg acccttaagc
      661 ttacaatttt gttctttgat ttattttgta gaacaatgta taatgtacct tatatgaaga
      721 agggagacta aaatgggtta ttacaatgat aatgataata ataaaataag acgtaaaaaa
      781 attagtaaca acaaagatag taataaaaaa tctaaacatt cttatacttc ggcaaataaa
      841 aaaaataaaa gtcctaatac aagttccagt aaaagaatga gtgaaaaagt agattcacag
      901 aatcttataa aaagagatag agatagtttt acaagagata atgtaagacc tttaaataca
      961 gattacaatc aaagagaaag aagaagagtc gagtataaac aaagagagaa aaaattaaaa
     1021 agtaaaaaaa gaaaaaaaca aaaaaggatt agaatagtta gggcattatt atctattatg
     1081 ttagttgcac taattatatt ttcagtggca ggtgcaatat ttacagtttc agctataaaa
     1141 gggtcaccag aagttactaa aaagttaatt aggtctaaat atataagtag tgaggttgta
     1201 agtataaaac aaatgccaaa tgatttaaaa catgcaatag tttccataga agatgagaga
     1261 ttttataagc ataaaggagt agatgttatt tctctagtta ggtcagtatt aaacaatgtg
     1321 tttacagata caacacaagg tgcaagtact atagatatgc aagtttcaaa aaacctgctt
     1381 actagtaatg ataaaactat gaagcggaaa ataagggata tgtataatgc taagcagatg
     1441 aataagatta tgactaagga tgaaattctt gaggcatatt taaataatat gtatctaggt
     1501 aaaaatgcat atggagctgc aaagggagct gaagtctact ttggtaaaaa agttagtgaa
     1561 ttaaatttag ctgagtgtgc tatgttagct ggtatgacaa ataatcctgc aaggtatatt
     1621 gaccatggag aggctaagaa gagacaagtg attgtactgt ataagatgca tcagcttggt
     1681 tatataactg ataaggagta tagaatagca aaagctgatg acactccatt taaatcagaa
     1741 attgattaaa attgtaagaa tatagtattt tcaatagttt aaacaaacaa aaatgtaata
     1801 aaaaaggtga tttgccaccc atttgccacc ataattataa tctaatggca aattgtttta
     1861 atgtacaaac tagataatag aatattgttt taaatgctat attatgctaa aatttaattg
     1921 tattttgtaa attaaaaaac ttataattta gttttctagg gttccgcatt ttataatggt
     1981 caggtccaag agaaaacgcg caattgttgt gtacacggaa ggataaaagc ctgggagata
     2041 tttaataata tttctatagg cttttttatt tttacatatt ttaaatttga ggaggttttc
     2101 taatgaaaaa atttgttatt gaggatgcat tttggaatct atttcctagt gcaaaaattg
     2161 gagttattat ttgtaatgat atagataact ctataaaaga tgaagactat aagaaaataa
     2221 ttttgaaggg agaagaagaa gcattaaaat acttggagga ctcagaattt agcaacaacc
     2281 aagtgattaa agtatggaga gaggcattta aaaaatttaa aacgaaaaaa ggagcaaggt
     2341 cttctattga agcattatta aaaagagttc ataatggaaa taatatagga actattaacc
     2401 ctttagtgga tatctacaat tttatctcat taaaatatgc attaccttgt ggaggggagg
     2461 atatagataa atttatagga gatattaggc taactagagc aattggtaat gaggagttta
     2521 tcccactagg gacagatgaa aattcatttc catatgaagg agaaataatt tataaagatg
     2581 atggtggagc aatttgtaga tgttggaatt ggcgtgaagc agtgagaaca atgcttactg
     2641 aagatactaa caatgccttt ttatgcattg aattagtaga tgaaagtaga tttaaagaat
     2701 ttgaaaatgc tttaaatgaa ttagctaaaa tagttcaaga aaaattgggt gggaattgta
     2761 aaatttcaat tttagatatt gataataaag aggtatcagt ggattaatct ggagatgctg
     2821 ttgtaatgga ctattctagt tataaagata cttatattta gggtaataag ttctatttta
     2881 gatgtaacga ttatcaaagg atataatata gaaaataaaa atgtagaata taaaaagcct
     2941 ttaaaataag aaaaaaatgt attttaaagg cttttattat actctttttt atttgcataa
     3001 ttaacaataa aaatatattg ttaattataa tattttta
//
LOCUS       DAMKPG010000196         2935 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMKPG010000196 DAMKPG010000000
VERSION     DAMKPG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.203"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            391..2202
                     /locus_tag="PC621_003644"
     CDS             391..2202
                     /locus_tag="PC621_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF4110752.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2290..2871)
                     /locus_tag="PC621_003645"
     CDS             complement(2290..2871)
                     /locus_tag="PC621_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110753.1"
                     /translation="MLYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1104 a          300 c          412 g         1119 t
ORIGIN      
        1 aaataaattc aaagagttaa attgtaggta aatagtattt taattattta ttaaaatata
       61 gattatgtat atgccaaatt taaaaagaga cataaagaca ttatacaaat atattattgg
      121 ggtgatattt tataagaaaa gaagtgatgc agtaaattaa ttgagcaatt taaaaatgat
      181 ttaatgatag tgttgatgaa tgtttaaaaa gaaattttag aaaaacgtat attgctgttt
      241 gtgttggagg tgatgaattt gtataattta tagggtataa atgattaaac tacttaagat
      301 aaatacatat cactgtaaaa tattgataat cattttttat aagagattga ttatttgcaa
      361 attatatata ttacaaatga ggtaacaata atgaagaaaa aatatgattt tttaatttat
      421 tctgctattt ggctagttat tattattttt tttatatttt ctctatatca tagtatagag
      481 catattaaaa ttataagtca cactggtact attcgcagtg aaactcaaaa ggttgttaag
      541 caagaattga ataatgaaag aaatgatgac ttgataaaac gactggataa tattctgata
      601 aagctaagaa caggtaatgg agaaaatgga tttcaaagat gtgataataa agaatttcaa
      661 caaaaactaa atcaaatgga ttctatgtgg gaatctatga agaaagaaat tataaaagtt
      721 agaaatggtg cttcaggtga taaattatat aaattgagtg aagagtattc tgtgttgtct
      781 aatcaaattg tttttatatc tgaaaaacac tctaatgcaa aattgtattc ttttgctact
      841 gcactattta tttatcttat attctcaaca ataagtcttt taatttggga atattataat
      901 aaaaaaaggt ttaaaagaat attttatact gataatttaa caaaaataaa aaatcaggtt
      961 gcatttgaaa acagagcgat tgaaattttg tacaatgcat ctaacaaaga gtatgtactg
     1021 ctaaatattg atattgacaa ttttaaatat attaatgata cacatggata tgaatatgga
     1081 gataaagttc taataatagt tgcagctgca ctttctaaaa cttttaatat caaagaaact
     1141 tgtgcaagaa ttggttcaga taattttgta atacttgcaa aatatagaga ttctctttta
     1201 gaggatattc gggaaatgct aactaatgct ataatctctg aattagatat gaatgtaact
     1261 caaactatat cttattgtat aggagcgtac ttagttgaaa ttgataattt aggttataaa
     1321 tccatcaatt ctataatgga taaggcaaat attgcacaca aggtaagtaa aacaagagga
     1381 atttcctcca cagtatggta taatgaaaat ctgttaaaac agttacaaat ggaaaatagt
     1441 atttataatt atatgtataa agcgatagaa aatgaagaat ttcatatgta tttgcaacct
     1501 aaatttcaaa tatcttcttt aaatgttgta agcgcagaag ctcttgtaag atggttttct
     1561 ccagaacttg gttttttatc accagatgaa tttattccat tgtttgaaaa aagtggattt
     1621 attattgaat tggactttta tatgttaaaa aaagcgtgtt catttgtaaa aaaaacgttt
     1681 atgaaaaaaa atcaatatac atatccgatt gcagttaatt tttcaagggt tactatttac
     1741 caaaatagct tttatcaaag gtttttagat actgttaaag aatatgaaat tccttttaag
     1801 tatatagaga tagaggtaac tgaaagtgct tttaatgaaa tatctcaacc agttatttcg
     1861 attttagagg agcttaaaaa gttaggattt ttaatttcga tggatgattt tggatcagga
     1921 tactcttcat taagtctttt atgttctcta tctattaatg gattgaagtt agataaaagc
     1981 ttacttaaag aaacatttaa ccgggagaaa gtatatagta ttattcagtg tattattgaa
     2041 atgtcacatc gtataggcat gtctgttgta tgtgaaggta ttgaaactaa aaaagacttg
     2101 gaatttttaa atacagttaa atgtgatgta ggacaagggt tttatttctc taaaccgata
     2161 gaagaaaaag aattttttaa caaatatgta actaaaaaat aatataatta tttttatcag
     2221 tctatacatt gagtcatttt atatttcgtt aaatgactca atgtattgtt atgtatttta
     2281 aaatataaat tatttaaaac gttttttgta cttatataga aattcttcta tattttttga
     2341 ttgttctctt atttcaactg cttctatatc tagtaaataa agtatagaat catttgtaag
     2401 ttcaacatat tcaccatctt ctgaaccgag tgcatgtctc tcaattttta atcctttttc
     2461 ttcataatat ttttttgttt ctatatataa tgattctcgc ttttgtatgt agtctttcac
     2521 ttcattattt ggaaagttat atgaatccac taatgtttcg ccatttataa aagttttctc
     2581 ggtagcaact ttttcatccc aataatggat ttcttcccaa gtaaaaaata tctcagttcc
     2641 atctggaatg aatgaaaacc tggtaataat atataaataa ttattgaaat ttacttcata
     2701 atattcagga atatcttcaa attccatata gtaatttgta taattaaaat tactattatc
     2761 tttttttaca aagggtattt tttcaataaa tttatatatt gattgttcat ttttaaaaat
     2821 tcctatatca tagcttcttt caaattgatt aatttttaat aaatacaaca ttctttctcc
     2881 ttttattcaa aatataattt ttataaattt actaattttt agctttttta attat
//
LOCUS       DAMKPG010000197         2885 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMKPG010000197 DAMKPG010000000
VERSION     DAMKPG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.204"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            403..897
                     /locus_tag="PC621_003646"
     CDS             403..897
                     /locus_tag="PC621_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF4110754.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            1294..1444
                     /locus_tag="PC621_003647"
                     /pseudo
     CDS             1294..1444
                     /locus_tag="PC621_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1920..2108)
                     /locus_tag="PC621_003648"
     CDS             complement(1920..2108)
                     /locus_tag="PC621_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110755.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            2270..2620
                     /locus_tag="PC621_003649"
     CDS             2270..2620
                     /locus_tag="PC621_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF4110756.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2622..2822
                     /locus_tag="PC621_003650"
     CDS             2622..2822
                     /locus_tag="PC621_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110757.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1064 a          334 c          364 g         1123 t
ORIGIN      
        1 tactgataat tcatataatt attttgattt tatagataca cagttagatg attttataat
       61 agagcataaa aaaaatgtag tataatcttg tttatatcaa gttatggtat tggtaagtaa
      121 gtataattaa atagacatta ttttctgggt cctcttttta ttttcatctg cttttaaata
      181 atattattga cattgatatg actgtagttt tctttatttt aatccattgc ttatatcttt
      241 tctaacaaga aatgtcattt taaatcattt ctataaattg taaaataata gtatatttgt
      301 catagaaagt gatttttttg tctatatatg gtaaaatata gaaagtagaa atcaacaaaa
      361 taaaaattta tatattatta attttatatg gaggggaaat aaatgaaaaa taaaaaagga
      421 tttactctag tggaattatt agtagtaatt gctataatag gaatattggc aataatagca
      481 cttccagcat tatttaaaaa tatagaaaaa gcaaagatag ctaaacttga agctgatata
      541 agtgcaataa aaagtgcatc tcttagttac tatgctgatg aatccaagta tactgatgga
      601 ggaatgatat catgggtaaa gaaagatgga aaaataataa taaatggggg ttttaaagat
      661 gacccattag cagataaaat agaaaattta gggatgcctt ataatggttc atatctgtta
      721 atgtcatctc ctggtcatga aaaatatcta gaattaagca tacttccaga aggagaaata
      781 agcaaaagtg gtctagataa attaaaaaat gattatggaa atttaataga cataacgaac
      841 gatcaaaata aaataaatat tgtaataaaa cttttaaata ataaatcgaa tacttaaata
      901 caataaattt gatttaataa ttactgatga aataaacaat cttgtttgga gaaatgaaaa
      961 aagcactccc caagaataga atgcttagtt ataactacag aagtgtattg gcatacactt
     1021 ctatttttta ttatatcaca taatttatgt tgctaaaaat acctatttaa gttgttttta
     1081 aattgtgttg agtaatattc ttgatagttt atacattaat gaatttcaaa aaaataagca
     1141 ctctttataa taaaaagtac tttagtgtat gttaatgtcc tatctaataa aattgtaaca
     1201 tgtcttatgc tttagtatga taattttcat tctctttatt gttgcaactt atactatagt
     1261 tttgctaatt tatctgcaaa cacaacttct ttattgcatg tgtatcaacc aaaaacatca
     1321 gaatacttac ttaatacatc tgcaaaatcg tatctttcat ctatagtatt cttatttgac
     1381 aatataacaa aaaatgaatc gagatatgcc ttttttataa ttagatatat tttataaatt
     1441 ctagcatatc atttgttatt ttctgatatg ctattgatgt tttaaatctt ctatttattt
     1501 tattattttt tacttttcca attgtacctt tataggagtt atatttatgt gatttatgaa
     1561 aaaatgctat gcatttaagt tctaattttt tgataataca tctaactttc ttttgattaa
     1621 taatgatacc actattttta atgtggcttc cctgaggcac acatcttttt taaaaactca
     1681 agctcatctt ttaaaaattt attttcatat tctaattctt gttctcttgt taatttattt
     1741 tttctttagt gtttttgttt ttattcatta tggtcttcct ttcattttga aaaaggcatc
     1801 aacaccacta tgtaaaaatt tttgttttca tgttgctata actgtgctat tgtttattcc
     1861 aaaatactta gcgatttatt gtacactata cttagttatc atcatatagt ttataacatc
     1921 taacttgaac catatagtat agaaaacttt tttattttta gtgcaccctc tcctaatata
     1981 ttaacccatc tttcaacttg agaatggtca taaactccat attgttttac tattaaaata
     2041 tctcttcctt ctctattcaa ataaaattta gctactttta atttgaaatt aaaatcatat
     2101 ttagtcatac ataaacatct caagcgttag atttttactc taacaattat ggtgcagtac
     2161 aaatattgta taatactgat tttctattga caaaagaata taaaagagat atatttgtat
     2221 tataaagata caaatatatc tcttttaaaa ttactaagga ggataaaaaa tgattcaaaa
     2281 aaaaattttt atgggttttt gtggtttcct tggattttta agtcttagat atttttcatc
     2341 tggcaatgtt actgatctta catatattgg attttttgct tttttctcaa actttattat
     2401 agcaaaaata aatggtgata aagcagatga aaggtatgta caagatgaaa aagcagctat
     2461 ggcatttaca ggacagcttg ctattataga attattcatt ttatggtgta ttactatagt
     2521 gtctaggaat gtagaattaa tgtgtgtttt actatcaatt acatatgcaa tcactttaaa
     2581 tgtttatgct ataaaacttt atattttaga ggaaaaataa tatgcccaaa ttcatttgta
     2641 acctaaaaaa atatagacaa ttaaaagaat taactcaaga acaattagca gaaaaagtag
     2701 gagttagaag agaaaccatt atgcgtttag aagcaggaaa gtataatcct tctttgaaat
     2761 tagcaataga tatatcaaaa atagtagata cacctattga tgaattgttt atttttgatt
     2821 agtttttgca ttattaaaaa tgtgttttaa atacaaactt aaattcaatc tttaagtcac
     2881 tcctg
//
LOCUS       DAMKPG010000198         2741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMKPG010000198 DAMKPG010000000
VERSION     DAMKPG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.205"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            160..1083
                     /locus_tag="PC621_003651"
     CDS             160..1083
                     /locus_tag="PC621_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF4110758.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1058..1885
                     /gene="panB"
                     /locus_tag="PC621_003652"
     CDS             1058..1885
                     /gene="panB"
                     /locus_tag="PC621_003652"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF4110759.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1904..>2741
                     /locus_tag="PC621_003653"
     CDS             1904..>2741
                     /locus_tag="PC621_003653"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF4110760.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFE"
BASE COUNT         1085 a          336 c          457 g          863 t
ORIGIN      
        1 tgaaaatggt aagatattta tggatggtat cttgttataa gaactatact ttaaatgttt
       61 ttaattattt tgtcttatgt aactcatatc ctcaggaatg agattagaaa ttaagatgtt
      121 gtatcacaaa gtggaggtga tactattaaa ataggcttta ttggttctgg aaaagttgga
      181 acatctttgg caaattattt tttatcaaaa ggttattgtg tttcaggctt ttatggaagg
      241 aatcaattat ctttattaga atcaataaat ttaacaaaaa caaagattta cagcaatttg
      301 agagatataa tttatgaaaa cgatatatta tttattacaa catcagatga ttctattgaa
      361 attatagaca aaaaactttc caaatttaat ttaaaacata aatacatttg ccacacaagt
      421 ggctctttaa aatctagtat attgttctgt tcaaaaaaag caggtgcatt aatttattct
      481 atccatccta tatttgcatt ttccaataaa aataccaaca taaaaaaaat gaaagacatt
      541 tgtttttcca tagaaggtga taatgaacaa gatgatttag ttattcaaaa tttcatagat
      601 ggtttgggaa accaattttt tataagagat aaggatactt catccactta tcatctagct
      661 aatgttttag tttcaaattt agtattatct ttactagata taggagtaag ttactttatt
      721 aaattaggtt tatctgaaga ggaatcttta aaagctatca gtcctttagt aaaaaaaaat
      781 atagaaaata tacttgataa tggctttact aaagctctta ctggtcctgt ttctcgtgga
      841 gatataacac caataaggaa acatctatct gtattgaata aaaaagatga ggatatctac
      901 aaaatactat ccttaaacct actaaaaata attgccttag gaaatgacaa ttcattaaaa
      961 ggtacaaaaa atattactat agaaaatgca atcgaaaatt taataagtaa atctgaaaaa
     1021 tacaaagaaa tttacaaact tttaggagga aaaaacgatg aaaaatacca tacagacttt
     1081 taagaacgct aaatatgaag gaaaaaaact atctatgctt actgcttatg actactcaat
     1141 tgcaaaaata atggatgaat gtgatataaa tggtatttta ataggcgatt cattaggaat
     1201 ggtcataaaa ggtgaagaaa acactctatc tgttactata gacgaaatta tatatcatac
     1261 aaaagctgta aaaaatggtg caaaaaatgc cttaatcgta agtgatatgc cttttctatc
     1321 ttaccatgtt tctatagaag atgcagttaa aaacgctggt agacttataa aagaaggagg
     1381 agctcatgct gtaaagttag aaggtggctc aaacgttata aagcaaatag aaagcattgt
     1441 aaatgctcag atacctgtaa tggggcattt aggattaact cctcaatcag taaattcatt
     1501 tggtgggttt aaggttcaag gaaataccag tgaaacagca agacaactta ttgaagatgc
     1561 taaattaata gaaaaagcag gtgcattctc tattgtcctt gaaggagttc ctacgaaaat
     1621 agctgatatg gtaactaatt ctatttcaat tcctacaata ggtatagggg caggaattaa
     1681 ctgcgatgga caaattttag tttatcaaga tatgttggga gtgtttggag attttgttcc
     1741 taagtttgta aagcagtatg caaatatagg agatataatg aaagattcaa ttaaaaatta
     1801 catcctagaa gttaatacag gtgcatttcc acaagaaaaa cacagctttt caataaatga
     1861 atctgagtta gaaaaattat actaatatta aggatggtac aaaatgcttg taaaagaaat
     1921 taaattacta agaaatataa tcaaagattg gagaaaacat ggctattcta tagggcttgt
     1981 tacaacaatg ggatttttac atgaaggaca ccaaagtctt ataaaaaaag ctgtaaaaga
     2041 aaatgataaa gtagtagtga gtgtatttgt aaatccaact caatttggac caaatgaaga
     2101 ttttaatagc tatccaagag atatagataa agattttaaa tattgtatgg atagtggagc
     2161 aacagtagtc tttaacccat caccagaaga aatgtattta aaaggtaatt gtactaccat
     2221 taatgtttct ggacttacag attttctttg tggagctaaa agacctgttc attttggagg
     2281 tgtctgccta gttgtttcaa agtttctaaa tatagtaaca cctgataaag cttattttgg
     2341 agagaaggat gcccaacaat tggctgtaat taaaagaatg gtcaaggatt taaacataaa
     2401 tacagaaata ataggctgtc ctataattcg tgaaaatgat ggattagcaa aaagttcgag
     2461 aaatacatat ctctctgagg aagaacgaaa atctgctcta atattaaata agagcttaag
     2521 tttagcaaaa gaagaattag taaaaggcaa tctaaatcca aagaatataa aagaattaat
     2581 aacagctaaa ataaattctg aacacttagc aaaaatagac tatgttgaaa ttgtagatag
     2641 tgagacatta caacctgtta agcaaataga acattctatc cttgtggcaa ttgctgtgtt
     2701 tataggaaaa accagactaa ttgataattt tacatttgag t
//
LOCUS       DAMKPG010000199         2623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMKPG010000199 DAMKPG010000000
VERSION     DAMKPG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.206"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(225..1475)
                     /locus_tag="PC621_003654"
     CDS             complement(225..1475)
                     /locus_tag="PC621_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF4110761.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1507..2448)
                     /locus_tag="PC621_003655"
     CDS             complement(1507..2448)
                     /locus_tag="PC621_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF4110762.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          793 a          499 c          244 g         1087 t
ORIGIN      
        1 aatacatatt taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat
       61 tatagcatag aatatgacta tctatatcct aaaattatcc atttctaaaa aaaggtaact
      121 attattaaga atatcaactt atagaattta tattccacat acgattttca atcaaaaaaa
      181 caagggctaa aaagtgaatt gtacactttt tagcccttta ttaattattc atttatttca
      241 tgtaagaata gcccacatct tactttaggt tcaaaccatg ttgacttagc tggcattatc
      301 ttatttgcat ctgctactct tattaaatct tcaatactag taggatacat tgcaaatgct
      361 actttcatat cttcattaac tcttttttct aattcttcaa gacctctaat tccaccgata
      421 aaatcaattc ttttatctac tctaggattg tcaattccta aaatagcatc aataacatag
      481 tcttgtaata tagaaatgtc taatgagtca acagggtctt ctatttctaa taaattttta
      541 tttgctttca ttttatacca cttatcacct aggtacattc cgaaagttcc ttttttgtca
      601 ggtttatatt tttcttttcc ttctatttct tctagttcaa aattttcttt tattttattt
      661 ataaattctt cctcgcttaa accatttaag tctttgataa ccctattata atccaaaacc
      721 attaaattct catctggtgc aatcattgct atgaagtaat taaattcttc tttgccagta
      781 taatttggat ttttagctct catttctttg gctacatttt ctgctgaagc tgtcctatga
      841 tgaccatctg caatgtatag ataatctaat ttttcaaatt tatctactag gttatctatt
      901 atatcatcac tatcaatctt ccatactgta tgctctatct tgtcatctgt tatgaaatca
      961 tataaaggtt cattattatc cataaaatca ttgattatat catctatcac cctttgatgt
     1021 ttgtaagtta caagtatagt tcccgtatta gcatcacagt attttatgtg tttagttcta
     1081 tcaatttcct tttctggtcg agtgtactca tgttttttta ttgttccatt caagctttca
     1141 tcaactgata tacatgctac tataccaact tgagctctac catccatgat ttgtttatat
     1201 atatatatac actctttgtc atcttttact agaatttctt gttttcgaaa ctcatataaa
     1261 ttttctcgcg cttttaaata aactttttca tcatataaat ctatattttc atctaaatca
     1321 atttctgatt tatctatatg caaaaatgaa tatggattcc cttctgcaat tcttctagct
     1381 tctttactat tcataacatc gtaaggaagt gctgctactt tatctactat atatttattt
     1441 ggtctaatcg ccttaaatgg tttaatcgtt gccattacta atttcctcct aattatacaa
     1501 aatattttat aatgctaccc ctattagatt gttgaagtca agaaagaaat cttgaatcac
     1561 atttactatt tcgtctccta ttctttcttg agcttctact gtagatgccc caatatgagg
     1621 tgtaggagaa actcttggat gatttactaa gtctaaattt aatgtaggtt cttgttcata
     1681 aacatctata gcagctgctg ataatttacc ttcatctaat gcttttagta aatctttttc
     1741 atcaactaaa ccgcctcttg cacagtttat taaataagcc cctttcttca tcttatttat
     1801 ttctttttca gttattactg cccccttatt cttatcaaat ggtatgtgta aagataagaa
     1861 atctgcattt tctaaaactt cttccatatc acagaattta taattgttaa aaccttgcgc
     1921 ctcacccatt atgtcagtat atataacatt catacctaat aattctgctc tctttgcaac
     1981 ttcttttgca attcttccaa aacctataag tccaagtgtc tttccattaa tttctgtacc
     2041 tttatacttc ttcttctccc attttccatc tctcatagtt acatttgcag tatttatgaa
     2101 tcttgcaaga acaaataatt gacctattgt aagctctgca acagatatac tgcttgcatt
     2161 tggagtattc ataactttta tgccattagc ttgagcatat ttaacatcta tattatccag
     2221 acctactcca cctcttacta tcaacttcaa ctttttaccc tctaatgctt tatctattac
     2281 atctttagta actttagtag ctgaacgtac tacaagtaca tcaacatctt taagtttatc
     2341 tcctaaaaca tctttttcat aaaactgctc tatcacatta aaatctaact ctctcaattt
     2401 tctcgctgct tctttctcta ttccatcagt aactaatata ttatacataa caaactcctc
     2461 caatttttat tcacttaatt tttccaattt ttatttttta aagtattttc atcaataaaa
     2521 aactttactt aatttaactt tctgtttata taattttcaa aatatttttt tattaaagtc
     2581 atatcaaatt ttcctattgt ttgttaaaaa tttaatatgt aat
//
LOCUS       DAMKPG010000200         2427 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMKPG010000200 DAMKPG010000000
VERSION     DAMKPG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.208"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(85..1686)
                     /locus_tag="PC621_003656"
     CDS             complement(85..1686)
                     /locus_tag="PC621_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF4110763.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            complement(1717..2061)
                     /locus_tag="PC621_003657"
     CDS             complement(1717..2061)
                     /locus_tag="PC621_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF4110764.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
BASE COUNT          735 a          424 c          210 g         1058 t
ORIGIN      
        1 ttcaattgtt tttgttgtta ttccaccaat tgattttaac cataaataag tatctctttt
       61 ttccataaaa tccacctcaa aaaactattt aaaataacta taataagcct ttctaaatga
      121 aaaagcttct ataatacact gtgaatttat caagtctctt tcttctaaat ctgctattgt
      181 tcttgccatt tttaaaagtt ttgtataact tctactgctt aggttgtatt tattaaatat
      241 catctcagcc gtttttgaag cttctttttc cagtttacaa tatttaaata aattgtaagc
      301 tttaatttca ctattatttt ttatattatc tttcttaaat ctatttatct gtatttttct
      361 agcattttct actcttctct ttattttttg agagctttct tcttgtttta aagaattaaa
      421 atctgaatat tttatagaat taacttctac aaaaatatca aacctatcta acaatggacc
      481 tgatatttta tttatgtatc tatcaatttc gttacttcta catctacact ctgtctctga
      541 catataataa ccacatggac atggattcat agctgcaact aacagtactc tacaaggata
      601 ttttgcactg tactttactc tggatatatt tacatatcca tcttctatag gttgtcttaa
      661 agtctcaagt atttttctat caaattcggc tatctcatct aaaaataaga tacctctatg
      721 tgctaaagca atttctccag gtcttgcatc cattcctcct ccaattaaag attgctttgt
      781 agttgtatga tgaggtgctc taaaaggtct tttatcaatt attcctttac tttcatttat
      841 catacccaat atactataaa ccttacttac ttctatcatt tcttctatac ttatatctgg
      901 taaaattgtc cttactcttt ttgcaatcat agtttttcct gagcctggag gtccaatcat
      961 aaacatatta tgatttccag ctgctgcaat ttctgcactc ctctttacaa aataatttcc
     1021 ttttacatct ttaaagtctt catcatattt actttcttca ttattaatct tagtatattt
     1081 gcaatctata taactattat ctttttctaa ttctccgttc tcttttcttg acttatcatc
     1141 caaaaaagac attatactta ctttatcgac ttttatctct tcgtttaaaa aatcgacaca
     1201 ttcttttaaa cttttaatag gaattatatc aatttcatct actaatagac tttcttttat
     1261 gttctcaatt ggtataaaaa tacgttttat gttctgagtt tttgcaccca taattatggg
     1321 caatatccct cttacttttc ttatttttcc atctaatgat aattctccta taaacatact
     1381 ttctcttatg taattttcat ctttttttat taactctctt aaaaggccta tacttataga
     1441 taaatctaaa aaagctcctt ctttttttat atctgctggt gataaattta caactattct
     1501 cgtgcttgga aatttatatc cactattaag aattgctgac tttactcttt ctcttgcttc
     1561 tttaatttct ttgcctggaa gaccaacaat attaaaacta ggtattccat tgcttacatc
     1621 tacttcaacc ttaactaaaa agctgtcgat tcctactaag ttgcttgaat taataatact
     1681 taacatattt aatcctttcc aaaaacatta tcttttttaa aaagcattca ttatgtgatt
     1741 tattcttttt tctaacaaat agacctctat gatgtcaaat cttatgggag tattatttaa
     1801 gttatttttt ataatatagt attttgcgac accaattatc ttttttcttt ttttaaaatc
     1861 tacagattca caagggtagc catactttat gttagacctt gatttcacct ccacaaatac
     1921 aacttcatct cctatatttg ctattatatc tacctcccct atccttaatc tataattttt
     1981 ttctagtatc tttgcacctt ttgatagcag ataattaact gctatttctt ctccaaaatc
     2041 acctttttct ttattattca ttatctactc caatatatta aatttttact tttaaattat
     2101 cgtcagtata caatttaact attcaatttt ttgaaattga ttttatttcc attgatatta
     2161 ataaatactt ctattgactt tattctttta tgatattatg ctagttttta taatgaaagt
     2221 ttatttttat ggaggaatgt ctttaatgaa cagaaaaaaa acatttattt ttatacgata
     2281 tttatctcta tttatattac ttataagtta tttaaaccat actgaaaata cattgtatcc
     2341 agctataaca atgattatat tatttctaat aataaataat caagttagat ttttttcttt
     2401 atcaagtgag aataaattta tacttat
//
LOCUS       DAMKPG010000201         2415 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMKPG010000201 DAMKPG010000000
VERSION     DAMKPG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.209"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            40..282
                     /locus_tag="PC621_003658"
     CDS             40..282
                     /locus_tag="PC621_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF4110765.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            285..1481
                     /locus_tag="PC621_003659"
     CDS             285..1481
                     /locus_tag="PC621_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110766.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1601..2326)
                     /locus_tag="PC621_003660"
     CDS             complement(1601..2326)
                     /locus_tag="PC621_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF4110767.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT          915 a          283 c          388 g          829 t
ORIGIN      
        1 ggggatggcc tgtggagaat tagtaagaca tattttagta tgcaaaattc tatgaagcag
       61 gaaccctgtg actttagtca tgggaggttc agatttaaaa catcagataa tagagttttg
      121 ataccagtag taatattttt aggcagtata gttacagctc tttgtgattt aatatcaaga
      181 gtaattttat caccagtaga attaccaata agcacgataa catctttatt aggagctcca
      241 attgtagtaa tgttattggt gaaaaggaag gatagggctt agacatgttg aaaactaata
      301 atttaagtgt aggatacaat aacaaggtag ttataagtaa tataaatgta gaagtaaaaa
      361 atggagaaat actatgtctt ttgggaagta atggagctgg aaaaactacc ctattaaggt
      421 ccttatctaa acttatatca ccaataaaag gtgaaatata tttgaatggt gtaaacataa
      481 attgtatatc aagaaaagca ttatcaaaaa aaatggcttt agtacttaca aatagattat
      541 taggtgattt aatgactgta caagatatag tcaatatagg aaggtatcca tatacaggat
      601 tttttgggag tctatctaag aaagatttaa taatggtaga tgaagcattg gagtctgtag
      661 atgccttaca tttaaagaaa agatattttg atgaactaag tgatggagaa aaacaaaagg
      721 tgttggttgc aagagctctt gttcaagaac cagaaataat tatattagat gagcctacaa
      781 ctcacttgga tataaaacat agattggagc tcataaacat actgaaaaaa ttaagtaaag
      841 aaaagtcaat atcagtaata ctttcattgc atgaaataga catagcatta aaaagttgtg
      901 ataaagttgc tcttataaaa aataataaag tcatagctta tggtcagcct gaagatgtag
      961 tagatgagga tattataaat tcgctttatg aattggatga taaaaacttt aatagtcttt
     1021 taggttcagt agaaattagt aataaatcta aaaatgaagt ttttataatt ggtggtgggg
     1081 gtaaagcaac accaatttat agagctttta ctaaaaaagg tataggtctg tattctggaa
     1141 taatacatga aaatgatatt gattatgaaa ttggtagaac tatggggata aagatgttta
     1201 cagaaaatcc atttgagccg ataagtgatg agagtttcga tttggctatt agaaatttaa
     1261 atgattccaa gattataata gatacaggat ttagtgtagg agagacaaat aagaggaata
     1321 tagatattat aaaagaggct ttaaaactgg ataaaaaagt ttattctttt agaaatagag
     1381 atgagagtaa gaagtattat gacagtttgg atagtaagat agaacatata gataaagttt
     1441 ctcaaataat caatagtgca gatataaata atttattata atttatgaaa taaaatgtac
     1501 ttatgtgcta tagaaaaaag agtatagatt ataatttttt tgaaattaca atctatactc
     1561 ttttgttatt ttgtttatat atttataaaa ctttagcatt ttatagataa ccaatagctt
     1621 cctctctagt taaaaaaaag tgaatccctg tagtcgattc tttccatcta ttttcattaa
     1681 aatcttcagc tacaatcgtt tctcctactc tatatataaa attttcatca gcatatgcac
     1741 gagcactttt aaaagattct ttaccatcta tactagtaat tgacagtact tttgccttat
     1801 cacatctaca agcatttgat gtagcagaca ctctttttgc gtcgctgggt attaataatt
     1861 gtaccattct gaaattaaaa cattttttat agcctataaa tgctcctgtt tcaggacaat
     1921 acaatttaaa aaaaggggta ctttcatctg atattactcc atttaaagta gcatgttcta
     1981 aattggaatt ttctaaatta gctcctctaa tatctgagtt agataagtca caaaatctaa
     2041 aatttgcacc acttaagtct gcatttttta atatagaatt ttttaatgaa caatccgaaa
     2101 gataagaatt attaaaaata gagttttcta aatctgcttt ttctaaattt aaattgataa
     2161 ggtcagacca cgaaaagtca atattactta agcagtatcc tcttaagtcc atattttcta
     2221 atcttaattc ttttaaatct gccttttctc cagcattatt gtttttaagc cataaataat
     2281 gtttctcaag tgttttttct aattcttgtt tagaaatagt tttcattaaa atcaccttca
     2341 taaatttatt tttatatatg tagtagcaaa atagtcatat atttaattat ataacaataa
     2401 ataaaaaact tctaa
//
LOCUS       DAMKPG010000202         2400 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMKPG010000202 DAMKPG010000000
VERSION     DAMKPG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.210"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(182..1474)
                     /gene="brnQ"
                     /locus_tag="PC621_003661"
     CDS             complement(182..1474)
                     /gene="brnQ"
                     /locus_tag="PC621_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF4110768.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          811 a          393 c          250 g          946 t
ORIGIN      
        1 aattataaat aaatcttttt tattttattg tctttataaa tcatactcct ccaaaaataa
       61 atttatcaaa tgagattaat aatgttcttt tctccacaat aatttaataa ttcttttaaa
      121 tataaaaagg aattgccata taatttaaag gcaattccac aattatcaat tagttttatt
      181 attatgcagt tttactgctt ttagatggaa taaagtttcc tattattcct gctattacaa
      241 ctggaactat ccagttaaat cctaatgaag caaatggtaa gctattaact agaggaattt
      301 ttacaccaaa acttgttgca actgttaata cacttactaa taatgccatg tatgcagcac
      361 atttgaacac gttgtcattt ttaattttat tactaaataa tgctagtata actagagtta
      421 tagttgctgg atataccata cttagtatag gagaagagaa ttttattatt gtacttactc
      481 caaagtttga tatgattgca ctaaatacac aaactactat aactatatgc tcatacttta
      541 attttccatc tgtaagtgtt gagaaatatt gaccagctgc tgaagaaaga cctatagctg
      601 ttgttaaaca tgctaatgca actataatag ccaataaaat tttacctgta gttccaagta
      661 aagctgctgt tattgcaact attaaagctg tttgttgaac atcttgtcca tattgttgag
      721 acacagttgc tccaagataa cataatccac cataaactaa tgccattcct attactgcaa
      781 ttacacctgc acttattgtg gctttcatct tttctttctc acctgtataa cctttagcta
      841 ttactgacat gattataatt gatgcaaata cactagctgc caatgcatcc attgtttgat
      901 aaccttgagt tataccttct gcaaatacat tttgaattaa agaactgtct cttatttctc
      961 ctaatggaga tacgatacct ttaactatta atactgctaa agctataagt aaagctggag
     1021 ttaatattga cccaactata tctacaactt ttgatggtct tattgttaaa accaatacta
     1081 gagcaaagaa tactatagag aatattactg gacttattcc agaacctatt gtaggcaata
     1141 tacccatttc ataagttgtt gctgctgttc taggtattgc taaaagtggc cctatacaga
     1201 ttaccattgc acatcctaat gcaatactta atcctttgcc tgctcttgag aacatcttcg
     1261 atgtgtctcc attgaactta gttgctgcta taactgctaa taatgccaaa ccaccatctg
     1321 caaaaaggaa tcctatgaaa gctattagcc aactacttcc agataaaact cctagatatg
     1381 gagggaagat taagttccct gcaccaaaaa acattgcaaa tagtgcaaat ccaattacaa
     1441 ttacatcttt aaaattgatt tgtttctcac ccatttaaag cacccccatt tgaattttac
     1501 taccttttac taaaaaataa gtccaatctc ttcattaaat gaaaaccttt tgacgagatt
     1561 ttatttaatt atacattttt gacttaaatt tgtcaattat gtttcataat ttataaaatc
     1621 ttcacaatat ttaatacaac tttcttaatt aaacaaaatc aacctgtata ctttaaacat
     1681 agttggtttt ttgttttttc tctaattttc cttgataaaa ttaactattc agaacaatta
     1741 aaatattaag actttttaga ttattcgttt tattattaag ttgtattgct ttatatactg
     1801 tatatatcac tctccttttt acattcatat aatcttttta attcaattta aattttaaat
     1861 attttgtgtt tattatataa tctgaaaaaa aagcagtcaa tagcattttt aattttttta
     1921 attttcaaaa ttatgattct taaaaaatcg ttttcctttt atcataacac ctattaacgt
     1981 taattcttgt agtattttgt atttattttg ctaaattgac agttaacttt attcaatttt
     2041 tattctactc acgtagattt acctcttgtc ttttagtctt aaaaccattt tttctgaaaa
     2101 ttccttatta tgatttatca acttattttt cttcatagca attactttct aacttccaac
     2161 ttacttaaac tttgcagttg ctagtattca cgtttacata cttatatgtc ataattaact
     2221 aaaaattctt aatatatctc tcaatgaaaa caatttactc atttatttaa tacatttata
     2281 taatcatatg tcttaaattt tgttttatta ttttattatt gtcacaattt tatttatatt
     2341 atatttttgt aaaaatcatt taatatgtat acttaaattt ataaaattac tctaaaattc
//
LOCUS       DAMKPG010000203         2399 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMKPG010000203 DAMKPG010000000
VERSION     DAMKPG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.211"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..344
                     /locus_tag="PC621_003662"
     CDS             <1..344
                     /locus_tag="PC621_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110769.1"
                     /translation="IKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQLEYKAN
                     WHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNEIHDRDINASINILK
                     EGLRLITIQNK"
     gene            628..1074
                     /locus_tag="PC621_003663"
     CDS             628..1074
                     /locus_tag="PC621_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF4110770.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            1095..2138
                     /locus_tag="PC621_003664"
     CDS             1095..2138
                     /locus_tag="PC621_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF4110771.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT          980 a          248 c          449 g          722 t
ORIGIN      
        1 taataaaaga gaacgatatt atttgcatag aagatttaca agtaaagaat atgattagaa
       61 atcgtaaact atctcgctta atttctgatg tgtcatggtc agaatttgtt cgtcaattag
      121 aatacaaggc taattggcat ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt
      181 cacaaatatg caataaatgt gggtataaaa acgaggaagt gaaggactta aatataagag
      241 aatggatttg tcctagttgt aatgaaattc acgatagaga tataaatgca agtataaata
      301 tattaaaaga aggattaaga ctaataacga ttcaaaataa ataacttatg taagaaccgt
      361 aggaactacg gggatagcct gtggagaatt agtaagacat atgttagtat gcaaaattct
      421 atgaagcagg aaccctgtga ctttagtcat gggaggttca gactactttg taacaatttt
      481 aacaaagtat gttgatataa tactattaaa ttaatcttag attagtttat tctattgatt
      541 tgtagtttaa atttatattc aaaaacattg tataaacata taatttatat agggttttat
      601 aattcatatg aaaagagggt agatagtatg aagttaatat ggttgatagt agcagtctta
      661 tttggaatag cagaaatgtt aacaccaagc ttaacattga tatggtttag tgtaggagct
      721 gtaatactta tatttttaag tagctttata gagagtattt tcttacaaat tttaatattt
      781 gcagtaattt ctatagcaat gttagtcgta gctactaaaa aaatagtaaa aaaagataaa
      841 ggctataaat ctaacactaa tttacaagca atgatgtcta aaaaaggtat tgttacagaa
      901 gagatttctc caaataacac aggtctagtt gtagttgaac atgaaacatg gacggctatt
      961 tctatagatg gtgaaaagat agaaaaaggt tctacagtag aagtattaaa aattgaaggg
     1021 gtaaaattag ttgttaaaaa agtagacgcc acagttaatg tgacaaacca atagaataaa
     1081 ggaggattta tagtatgggt actaaaattg ttttaagtat tgtattaata gtagtagtcg
     1141 tagcaataag tttaacgtgt ataagagtta taaaacaatc taaagtaggt ataataatga
     1201 gacttggtaa gtttcaaaaa gtggctgaaa caggtgtaca tttcttaata ccatttttag
     1261 ataaaatggc atatgtaatt gaccttagag aaattgtaat agattttcca cctcaaccag
     1321 ttataactaa agataatgta actatgcaga ttgatacagt tgtatattat aaagttacag
     1381 atccagttag atatgtgttt gagatagcta atccaatagc tgctattgaa aatttaacag
     1441 ctacaacact aagaaatata attggtgaac ttgatttaga tgaaacatta acatcaagag
     1501 atataataaa tgtaaaaatg agaactatcc ttgatgaagc aacagataaa tggggtataa
     1561 aagtaaatag agtagagtta aaaaatataa tgcctcctca ggatattcaa gttgcgatgg
     1621 aaaaacaaat gagagcagaa agagaaagaa gagaggcaat acttcaagca gaaggtaata
     1681 agtcagctgc aatacttcaa gcagaaggtg aaaaacaatc tgcgatatta acagcagaag
     1741 ccaaaaaaga agctatggta cgtgtagcag aaggtgaaaa agaatctgct atattagtgg
     1801 cagaaggtga agctgaagct ataagacaga ccgctattgc taaggcacaa ggtgaggctg
     1861 aaatgataaa aagaactcaa atggcaacag cagaaggttt aaaattagtt ttttcagcaa
     1921 tgaaagaggc tgatatagac aataatattc tagcattaaa atctatggaa gctcttgaaa
     1981 aaatggctga aggtaagtca acaaaactgg ttttaccttc agaagcagtt aatttcctag
     2041 gaacattcaa aggaataaag gaagttatga gtgatgataa caaagaagta cttgatataa
     2101 aagaagtttt aaatgataat gaatcattaa aaaaataaaa gagttttgtt atcataggaa
     2161 ggatatggaa attaaaggtc gaattaatta aatatagcac aaaaatgcac gttgaaaaga
     2221 agaggactta taaattttta agtcttcttc tttgttttta ttagaaagat gaaattataa
     2281 aaatgaatta acagaatctt tatacaattt atcctttact aatgtaaaaa gtgttatata
     2341 atattatata aaacatatag caaataagca tggttttagt tagtctattt atattatta
//
LOCUS       DAMKPG010000204         2358 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMKPG010000204 DAMKPG010000000
VERSION     DAMKPG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.212"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..824
                     /locus_tag="PC621_003665"
     CDS             <1..824
                     /locus_tag="PC621_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HDF4110772.1"
                     /translation="VNKIYEENEKWKEYNIQNTMSKYIGIPESEVIFISNLSQYVSFL
                     SELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEAFKQKIIYYDKEIKNR
                     SSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHSIVYFIDSISYNTDTI
                     KRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGCFLKVSSEDKGEFKEK
                     LKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSIKFYYDEIVGGNL"
     gene            824..2116
                     /locus_tag="PC621_003666"
     CDS             824..2116
                     /locus_tag="PC621_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF4110773.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
BASE COUNT         1053 a          218 c          341 g          746 t
ORIGIN      
        1 ctgtaaataa aatatatgaa gaaaatgaaa agtggaaaga gtataatata caaaatacta
       61 tgtctaaata tataggtata cctgaatcag aagttatatt tatatcaaat ttgagtcagt
      121 atgtttcatt tttatctgaa ctagataacg caggcgacta tgtatctaga gggcaaaaag
      181 attgtatgta tgaattatta ccatcattac atagaaaata taaaaaagac tatggtatac
      241 attcagatga atacgaggaa gcattcaaac aaaaaataat ttattatgat aaagaaataa
      301 aaaatagatc gagtgaagaa ttaagagctg aagggcaaca ttttggacta ccaacagaat
      361 atttagattt cacagaagct catttaatat cattattatt tgcaatagaa gactataaat
      421 atacaaaaca gcattcaatt gtgtacttta tagactctat ttcttataat actgacacta
      481 taaaaagatc tgagaagtta gtaaattatt cagatacatc tgcaatagat aattgtagta
      541 aattttcatc aagatcattt tttataaagg tagggaattc aaatgaaaga atacattttc
      601 aaaaaggatg tttcctaaaa gtatcatcag aagataaagg cgaatttaag gaaaaattaa
      661 aaaagtattg taaagtagta gttattaata aagatgctaa aaaagaaata ttaaaagaat
      721 tatttaattt aggtattaca tttgaaaata tttatccaga taaagataat gtagttaaat
      781 ctataaaatt ctattatgat gagatagtag ggggaaattt ataatgagag aacaatttaa
      841 aaattctata aaaaatatta aatcaaacag acctcttgta tcagtccaag atattgtgaa
      901 ttttattgat aaaggagaaa agtacttgaa attatcaata gatgaaatag atgaaatgga
      961 aattttacaa ggaatagtta tgagtcctga ttatcaacgt acatataaat catcaaacaa
     1021 ggaagaaagt tctataattg aatctctttt aattggaata ccaatacctg aaatatttct
     1081 tgtaaaaata ggtgtaaatg atatgcaaat aagacatgta atggatgggc agcatcgttt
     1141 gaatgctata tataggtatg ttaagaataa atatccactt aaaaatttag atatattagg
     1201 caatgaccca ctatatgata ataaaaggtt ttcagaatta gagaaaaaag ataaaattaa
     1261 aatattagga agtcatttat caattctaga atttgatggt tttgataatc cagaaataga
     1321 aatagaatta tttaaaagat ataacagaaa tacaaaacca ttagagatgc aagaaatcga
     1381 gatggcaaca tatttttcag aaactagtaa gtatataagt aaatttataa atactctaat
     1441 aaaagataaa tataaggatg aaatagataa atcagaatat aaatatgcta attacaaatc
     1501 agatttactt agaatatata acataactaa atctcgtaat gataaacaaa aaaatcatca
     1561 agaaatatgt ataatattta gtattttaga aaatgggcta caagaaaata tcagagatgg
     1621 agttacagcc tctaagaaat ttttagagta taagtcaatt cagtataaaa ataatgaaag
     1681 tgaaaaatta gagttattaa aagataagtt tgatgagttt aataggatga tactgaaaat
     1741 atctgagaag atagaatatc cattctcaac aagtttaata tctgatgata agcaaagaca
     1801 aagtaaatac ttgataggag tatctattgt gttggcttct atttgttatt attatgatat
     1861 agacttggaa aataattatt taatacaaga tattaaaaaa ataatatctt gttcacccat
     1921 agcagatata gaatataagg cgtcttcaac taatatgaaa aatatgattc tatacttatt
     1981 tataaagaat aaaatacaag ataataaatt tgaatcatta aatttaaaaa gagataaaat
     2041 taaagaaata aatactttag taatgaataa tgatggttat ttaaatgaac agacaaggct
     2101 attagaaata tattaacaag ttataaaata aatcatggta atgtaaaata atttgtgctt
     2161 ttaaggttaa taaaaatctt ctttatggca attatttaaa tataatcaaa tgtcgtaaag
     2221 gagatttttt atgaacgctt taagaaaaca attagccaaa gaaattatat cagatatcta
     2281 aaaaagagca tgtcaaataa aaccctaggt atttaaattt ttttagagct acttaaaata
     2341 aattatgcta tctctgta
//
LOCUS       DAMKPG010000205         2026 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMKPG010000205 DAMKPG010000000
VERSION     DAMKPG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.213"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            20..1732
                     /gene="ptsP"
                     /locus_tag="PC621_003667"
     CDS             20..1732
                     /gene="ptsP"
                     /locus_tag="PC621_003667"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF4110774.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT          786 a          213 c          395 g          632 t
ORIGIN      
        1 ttttttagga ggtagggtta tggcttacaa aggaataggt gcttctcctg gtgttgcttt
       61 agggaaagcg ttagtagttg aacatagtga attagtaatt gaaaaaaaat ctatagataa
      121 tgttgaagct gaaattgcta aattagaaga tgcagttgca gtttctaaag aagaattagt
      181 aaaagttaag gaaaaggctt ctgaagaatt aggagcagag gaagcagaaa tatttgaagc
      241 acatttatta gttttagaag acccagagtt aattggttct gcaatagata aaattaaaac
      301 tgaaagtgta aatgcagagt atgctttaaa tgaaataaaa gagatgtttg ttagcatgtt
      361 tgagtctatg gacaatgagt acatgaaaga aagagcggca gatataaaag atgttacaaa
      421 tagaatactt agacatatat taggaattaa agttgttgat ttatctgctt taagcgaaga
      481 agttgtatta atagcacatg atttaactcc atcagacaca gcaactatga ataaaaaaat
      541 ggttttagga tttttaactg atataggtgg aagaacttct catactgcta tcatgtctag
      601 aactttagaa atagctgcaa tcgttggatt aaatgatata acttcaaaag ttaaggatgg
      661 agattttgta gttttcaatg gagacacagg tgaagtaatt gtgaatcctg atgaagagac
      721 tataaataaa tatactgaat taaaagctaa gtatgaagat gagagaaaag cattacagct
      781 attaaaagga aaaccatctg taactttaga tggaaaacat gtggaattag ctggtaacat
      841 aggaacacct aacgatatag aaggtctaat aaagaatgat gctgaaggtg ttggtcttta
      901 tagaacagaa tttttataca tggatagaga ttctttccca acagaagaaa tacaatatga
      961 agcatacaaa gctgtattag aaggaatgga tggaaagcct atagttataa gaacattgga
     1021 tataggtgga gataaagaat taagttattt aagtatggaa cctgaaatga atccattctt
     1081 aggttacaga gcaataagac tttgcctaga tagaaaagat atatttaaga ctcaattgag
     1141 agctttatat agagctagta ttcatggaag attaagaatc atgttcccaa tgatttcatc
     1201 tttagaagaa ttgttacaag ctaaagaagt tgtaaaagaa gttttagcag aacttgattc
     1261 tgaaggtgtg gcttatgcta aagatgtaga aattggtatg atgatagaag ttccatctgc
     1321 tgctgtaata tctgatgtat tggctaaaca cgtagatttc ttctctatag gaactaatga
     1381 tttaattcaa tacacttgtg cagttgatag aatgaatcaa aagataagtt atctttacaa
     1441 tcaatttaat ccagctgttt taagacttat aaagactgtt atagacaatg cacataaaga
     1501 aggaaaatgg gcaggtatgt gtggagaatc tgccggagac caaaaaatga taccaatact
     1561 tttaggtatg ggattagacg agttctctat gtctccaata tcaatacttc ctgctagaaa
     1621 gttaataact tcagttaaag agtcagatat gaaaaaatta gctgatgatg tacttaatat
     1681 gggtactgct gaagaaataa aaagctatat agaaaagact tttaatatat aattaatgtt
     1741 atatagttac taaaattcat ttttctaaat aacaaaatta tattttacga attaaaaagg
     1801 agatgtttca aaataaaaat ttgaagcatc tcttttttat aaaagattac taagaattac
     1861 ttaatctttg taatagtcat tttgtgaaaa agcaaaactg cattgtgact atagtatata
     1921 tggatgttat tatcaataaa agtactatta aaatgaagat tcattctcaa atgatatcaa
     1981 aagtagttgt gatggagtaa gataatattt tgtgatttaa tagtaa
//
LOCUS       DAMKPG010000206         1983 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMKPG010000206 DAMKPG010000000
VERSION     DAMKPG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.214"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="PC621_003668"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="PC621_003668"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="PC621_003669"
     tRNA            301..375
                     /locus_tag="PC621_003669"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="PC621_003670"
     tRNA            380..454
                     /locus_tag="PC621_003670"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="PC621_003671"
     tRNA            460..535
                     /locus_tag="PC621_003671"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="PC621_003672"
     tRNA            541..617
                     /locus_tag="PC621_003672"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="PC621_003673"
     tRNA            628..702
                     /locus_tag="PC621_003673"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="PC621_003674"
     tRNA            717..801
                     /locus_tag="PC621_003674"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="PC621_003675"
     tRNA            811..884
                     /locus_tag="PC621_003675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="PC621_003676"
     tRNA            895..971
                     /locus_tag="PC621_003676"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="PC621_003677"
     tRNA            981..1056
                     /locus_tag="PC621_003677"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="PC621_003678"
     tRNA            1068..1143
                     /locus_tag="PC621_003678"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="PC621_003679"
     tRNA            1146..1234
                     /locus_tag="PC621_003679"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="PC621_003680"
     tRNA            1252..1342
                     /locus_tag="PC621_003680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="PC621_003681"
     tRNA            1351..1427
                     /locus_tag="PC621_003681"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="PC621_003682"
     tRNA            1513..1588
                     /locus_tag="PC621_003682"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="PC621_003683"
     tRNA            1649..1725
                     /locus_tag="PC621_003683"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="PC621_003684"
     tRNA            1732..1808
                     /locus_tag="PC621_003684"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="PC621_003685"
     tRNA            1812..1887
                     /locus_tag="PC621_003685"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="PC621_003686"
     tRNA            1895..1971
                     /locus_tag="PC621_003686"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          501 a          449 c          535 g          498 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atc
//
LOCUS       DAMKPG010000207         1961 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMKPG010000207 DAMKPG010000000
VERSION     DAMKPG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.215"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            172..1383
                     /locus_tag="PC621_003687"
     CDS             172..1383
                     /locus_tag="PC621_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF4110775.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1575..1835
                     /locus_tag="PC621_003688"
     CDS             1575..1835
                     /locus_tag="PC621_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF4110776.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT          826 a          176 c          342 g          617 t
ORIGIN      
        1 aagttagcca tataaaaaat attaaaaata tatacttttc aatatttttt tgataaaata
       61 agaatgaatt tgtgcaattt tatttttctc caataactaa aaatagtttt ttgaattaat
      121 atttgcttaa gaataaaaat cagaaacgtt tttgatatag taggtgataa tatgaaagga
      181 ttagttttag aaggtggagg aacaaaagga gcctaccaaa taggtgcata taaagcttta
      241 agagagcttg gtatagagtt tcaaggagta actggcacat caattggtgc tttaaatgga
      301 gcatatataa ttcaaaatga tattgaagta atggaagata tttggttaaa ttatgactat
      361 actcatttta tgaatattga tagagaaacc tatgagaggt ttaaaaatgt agatttcact
      421 actaaaaatc tcaatgcagt gtttgagctt ataaataaag ctagaaaaaa tgaaggtata
      481 gatataagcc cattaagaca actaatggag acaacattaa atgaagacat aataaggaaa
      541 tcaaaaaaag actttggact tgttacggtc tggtgggata aaaaaattaa tccatatcca
      601 ttgtatatcg aagatatacc taaaggaaga ttgattgatt atttaattgc tagttccagt
      661 ttgcctattt ttcaaacgaa catgatggat gataagttgt atcttgatgg aatgttttgt
      721 gataacatgc caataggact cttggaagac aaaggttatg aagatgttgt tgtaattagg
      781 ttgattgatg attttattgg gaaaatgaat ctaaatcgac atcaaaatat aaatataaaa
      841 actataatac catcagaata tctaggaggt tctcttaata aagatagaga taatgtagaa
      901 aaaaacatcc gactaggtta cttagatact atgaaagcat atgaaagatg cactggaatt
      961 aagtattatt ttgatgtaga gtataaatat aatgaagaat attgttttga taaatttaga
     1021 agtatgagca atgaaacaat acaatatatc tgtaatttat tgaatataaa aagagatgct
     1081 tcacttagaa ctgttatgga aagtgtaata ccaaagcttg gtgaggtgct tggattgcca
     1141 aaggactttt catataaaga tttgttttac tgtatatatg aaaagaaact tgaagaaaat
     1201 aatataaaca ggataaaatt atatgatttt aacaaggtta tagatgttgt aaatacaaat
     1261 attgattcta atagaaattt agcttctaat tttgaagtta gacctaaaat tagtattaaa
     1321 aagaacaaaa acacaaaatt attgacaaat tgtattataa aagacataaa aaatcaaagt
     1381 taaataaaaa acaaaaaaaa atttgtaatt atactcgaaa aaagtgtata ttagtagtat
     1441 aatatttttg tagcactagt gggaacaaaa aatgaccata ggacaaaaaa gtaccactta
     1501 gtaccattaa agtaaaaaac aggaaaacaa ttttaaaaaa tataaaatgt aaagtttata
     1561 ggaggattta aaaaatggaa aaagtagtta gtatcaaaaa tgcaagtgga ttacatgcta
     1621 gaccagcagg aatgtttgtt aagaaggcag ctgaatttaa atcaacagta gaggttgtag
     1681 caaaagacaa aactgtaaat gcaaaatcaa taatgggaat aatgagttta ggtttagctc
     1741 aaggagaaga attaaagtta gttgtaaatg gagaagatga agaagtagct ttagctgcaa
     1801 tggttgaatt agttgaaagt ggatttggag aatagatata ataatttttt aaaatttgat
     1861 atttagaatc taatattact taaagtaaag taacagagaa gtattaaaca tatacagacc
     1921 aacatttgta gatatttaat ttctttgtta ctttcatgtt t
//
LOCUS       DAMKPG010000208         1941 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMKPG010000208 DAMKPG010000000
VERSION     DAMKPG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.216"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(402..1730)
                     /locus_tag="PC621_003689"
     CDS             complement(402..1730)
                     /locus_tag="PC621_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF4110777.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          714 a          344 c          212 g          671 t
ORIGIN      
        1 aatttttgtt atcaaataaa attttctaat cttatttata aatcacgata ttgatcaggt
       61 aatattttct atataataca atattctatt ttatataaca gaaagattta attttcataa
      121 aacctatgta taatatttgt tatatagata tcacttagtt tgaaaaaaaa acactttaaa
      181 ataaagtatc ttaaagcaaa taatttttta gatgtttaat tttataattc aaaaactaaa
      241 aataaagtga ataccttaaa ataaactttt tagttcatag aacactcttt ttttataaaa
      301 taaaacatct ctttaccctt ttaacaataa aaaagagact tatctctatt ttgagacagt
      361 ctcttttatt ttaattataa agcatttgta aactacttgg tttatctttt tattcctcta
      421 gtcatataag catacactgg taagcctata gctgttatta caagacctcc tatagatatc
      481 atagtagttt tcatccctgc aaaacacaat tgattaacta caacaaatat accacttgct
      541 atagctatta taggaacaat aggatataat ggaactttat actctcttgg tatatttgga
      601 tgagtctttc taagtttcat aacccctata aaagttaata catagaatat ccaagtagca
      661 aatacagcta agtcagttaa taaattaaat tgacctgaca aagcatatat agatgcaata
      721 acagatacta atgctattgc atttgctgga acttgagcag aatttaattt agaaaatata
      781 ccatactttg gtaaagtctt atctgtagct aaagtatatg ccacccttga accagttagt
      841 aaaaatccat ttaatgctcc aaacactgaa attaatatac ctacagatat tatttttcca
      901 cccattgaac caaatataac ttcagcaact gctgatgcag gagaagcata atttgctaat
      961 tcattagctg gtaataccca aagatacgct aaatttatta taaaataaac agccataaca
     1021 actgacaatc cacctattat agcttttgga aggtcttttc caggattctt catttctcca
     1081 gctaaagtac caacattcat ccatccatca aaagcaaata atatagcaat taaaacttga
     1141 cctagtacac tacccaggct taatccttca ccaaccatag gtgtaagtat tggattattt
     1201 ccaccacctt taataaatcc aacaaccatt attaaaataa gtggtattaa cttacaaatt
     1261 gtagagacag tttgtatgac tcctccagtt tttgaaccta acatattaag ccctgcaact
     1321 attactataa ttccaattgc tattggaagt aaaagtgatg ggctaccaat tagtgctgat
     1381 gcttgttctc caaatataac acctaatgct gccatcatcc ctggataaaa taatacaatt
     1441 tgcatccatc cagttaaaaa acctaatttg tctccataaa tttcttttat atataccatc
     1501 attccaccag tttttggtat cgcaactgat acttctgcag ctgtaagacc agctgttata
     1561 gttatgattc cagctattaa ccatgccaat ataccaagac ctggagctcc tccagttaat
     1621 gtatagactg cttgtggttt aaaaaatacg cccgctccta taacagaacc aacaacagtt
     1681 gataatgctg ctgatacgcc aagagtcttt ttcagttcat tacttcccat tcttttcctc
     1741 ctcattaatc ctatttaatt ttaatttcat agttaaataa gcatatatta tgccaatata
     1801 tagaaaaata gcttttttat ttattaaaat aaaataaact ctacttagct ttcgtaatct
     1861 tttactttta gttatgttta aatataaaaa aattataaaa accaaaaatc caaatataat
     1921 aatatttata aataaacgta t
//
LOCUS       DAMKPG010000209         1883 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMKPG010000209 DAMKPG010000000
VERSION     DAMKPG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.217"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            159..1739
                     /locus_tag="PC621_003690"
     CDS             159..1739
                     /locus_tag="PC621_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF4110778.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT          734 a          168 c          370 g          611 t
ORIGIN      
        1 aaataattgt tttaattcaa aattttttgt tattttaaat tatttcaaaa ctcttagttt
       61 tagtgatttt atttatgata tttatgggat taaaagattt caatagatat ttttggtaag
      121 tatttaagaa gtctttaact ttataggagt gataatttat gctaataggt acatctaaat
      181 taagcagaaa tgttgatact gcatgtgtag aagaagtagg gattccaatg attgtattga
      241 tggaaaatgc ggttatatct gcaatgaaaa atatggatat agacatgtat aatagttata
      301 cagttgtatg tggtgttgga aataatggag gagatggtct tggaatagca agacatctaa
      361 atacatatgg aaaagaagta aacgtatttt tagtaggtaa tcttgaaaaa ctaagtgaat
      421 gctctaaaat aaattacaat gtacttttaa atatgggaat taatattata aatattaata
      481 aaaatgattt aagtgaaaaa aattatggag atgaaaatga agtagataaa attattaaag
      541 ttgagaaaca aaatattata aaaaataaaa atttaacaat ttttaaagat agagtatcta
      601 aaagtgatgt tgtagtagat gctatatttg ggactggatt aaaacgtgat ataactggta
      661 tttttaaaga agttattgac atagtaaacg ataatagcaa aaatacatat tctatagata
      721 ttccttcagg tatagatagc gataatggaa atgtattagg aacttgtatt agagcaaaca
      781 aaacgattag ctttgagttt tataaaagag gttttctaaa ctatgacact agcacactta
      841 taggtgatgt agttgtagaa aagataggtg ttccagattt tattacaaga aaataccatg
      901 atgaagagtt cataactgat aagacttttg taaaaaataa tattagaaga agagataagc
      961 atggttttaa aagtgatttt ggaaaagtat ctatagttgc aggttcaaaa ggattttatg
     1021 gtgcaagttt catagcaact gaatctgctg ttaagagtgg gagtggactt gtaacattaa
     1081 ttagtagtga agatgtgcaa gaaaaggtat ctattagact tacagaagca atgactttaa
     1141 attttgaaga agaaagatta aataaattac ttaattctag tgatgcaatt ggttttggtc
     1201 caggtatggg agataatagt caaacatttg ataaattact taagatagta gaaaattcta
     1261 attgcccaat agtattagat gcagatggat tgaacgttat gaaggacaga tgttataagt
     1321 ttttagaatg gaaaaataga tttgtaatta cacctcattt gggagaaatg gccaggttga
     1381 caggagattc aattgggtat ataagagaac atagagtaga tgttgcaaaa gaatttgctc
     1441 aaaaatataa tctggtggtg cttttaaaag gttatcaaac tgtcataaca gatggtaaaa
     1501 aaacatatat aaatcctaca ggaaatagct gtatggcaac tggtggaatg ggtgattgtt
     1561 tgcttggaat gattacttca tttataggtc aaggtatgga tatattggaa gctacagtta
     1621 gtggagcata tatacatggc tatataggag ataaattgag caaagcaatg tatacggtaa
     1681 atgctacgga tttaataagt aacatttcat taacaatgaa agagttgttg gatatataat
     1741 tgattaaaaa atagctacgc acacttgtga ctttagtcat gagttagtag ctaaaatagt
     1801 cagcatatag ggaaacctat atgtagagat aggatagaac ctatccaata ctacttgaat
     1861 tgctaaaatc ccctaaagct aac
//
LOCUS       DAMKPG010000210         1866 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMKPG010000210 DAMKPG010000000
VERSION     DAMKPG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.218"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            146..1195
                     /locus_tag="PC621_003691"
     CDS             146..1195
                     /locus_tag="PC621_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4110779.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1345..1692)
                     /locus_tag="PC621_003692"
     CDS             complement(1345..1692)
                     /locus_tag="PC621_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF4110780.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
BASE COUNT          702 a          247 c          286 g          631 t
ORIGIN      
        1 atattactaa aaaaactaaa cataaaactg aaaattatta ttaagttttt gtagaaatat
       61 tataagcata gatacatttt tgtaacaact acaataaatt tttaatacta tatactattc
      121 gtatgtctta tgggaggtat ttattatgtt taaaaaaata gtcgcaatag caagctctat
      181 cattgtaagt acaatatgtc taactggatg tgattttaac tctaatgaca aaaaagatga
      241 atcttcttcg tctaaaaatc tggataaagt aactatagca gaagttactc actctgtttt
      301 ttatgctcca cagtatgctg ctattacgaa aggattcttt gaagatgaag gtataaagat
      361 tgaccttata aatacccaag gtgcagataa aactatggca gctttgattt caggtgaagc
      421 aaacgtaggt ttaatgggtc ctgaagcatc tatatatgta tataatcaaa acaaaaacga
      481 ttatgcagtg aactttgctc aattgacaaa aagagatggt agttttctag ttgctaggaa
      541 aaaaatgcct aatttctctt acaatgattt aaaaggaaca gaaatattag gtggtagaaa
      601 aggtggagtt ccactaatga cttttgaata tgttctaaaa gaaaaaggtc ttactattgg
      661 agaaaatcta aaagcaggca atgttaatgt aagaactgat gtacaatttg gtgtaatggc
      721 tggagctttt gcaggtggag agggtgactt tacaacagca tttgaaccta cagcaacagc
      781 aatggaaaaa gaaggtagtg gatatatagt tacatctata ggaaaagatt ctggagaaat
      841 accttttaca gcatactcta ctacgagatc atacatgaaa aacaataaag atttaattca
      901 aagatttaca aatgctgttt ataaaggtca attatgggtt caacaagcat ctagcaaaga
      961 agtagctaag gctatgcaac ctttctttga agatatatct ttagaagatt taacaacagt
     1021 tgtagataga tataaatcaa tagatgcatg gtctcaaaat cctgtattaa aggaggaagg
     1081 tttgaagaaa ttgatgactg ttatgaaaga agctaaagaa ctagataagg aagcacctta
     1141 taaagatatc gtaaatactg agtttgcaaa caatgctata aaaaatatta aataaaatat
     1201 attagtttta gctagatatt ttcttgtttt tataatgaaa attaacaaaa atataaaaag
     1261 agccttttaa aagactcttt ttatatttga gattaaagtt tatataataa aatagatatt
     1321 aaagtactaa ctgtacttat atatctatcc tctgtattct gctccagcat taatttttgt
     1381 aatttgaacg gctaccattt cttcattgct tccattagta atttcatgcc actctcctgt
     1441 tggtacaaat acagtatcgc cgttttttac atcacattca tgctcaccaa gtttcatttt
     1501 accttctcct tttaggaaca caaatatttg ttcaccatta gggtgtctat gagcattaag
     1561 aacttgatta ggtttaaaat aataaacttc tgaagttata tcgttaccag caaatactgg
     1621 tatttttgga actgctgtat cattatattg agctaagctt ccgtctaata gattcttttt
     1681 attttcatgc atactagaaa tacctctttt ctatattata gatttactat tattatacca
     1741 atataagaaa atagtattct cactttacac aataggaata aaattctttt atttttaaat
     1801 ataagtaaat actattttta tagttagaaa aatataatca ttataagata taaaaaaatt
     1861 aacata
//
LOCUS       DAMKPG010000211         1766 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMKPG010000211 DAMKPG010000000
VERSION     DAMKPG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.219"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(110..1030)
                     /locus_tag="PC621_003693"
                     /pseudo
     CDS             complement(110..1030)
                     /locus_tag="PC621_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
     gene            complement(1319..>1766)
                     /locus_tag="PC621_003694"
     CDS             complement(1319..>1766)
                     /locus_tag="PC621_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110781.1"
                     /translation="IGSLNRNKARLKVARLQEHIANQRKDFLQKLSTKLIKENDIICI
                     EDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCG
                     YKNEEVKDLSIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT          566 a          262 c          213 g          725 t
ORIGIN      
        1 taatgataaa tatatattaa caaataataa aaaaattaat ttgattgtat ataaattagt
       61 ttgttattaa attagtatta aaaagttcaa aaatttactt agaaagaatc tataattcga
      121 tttctatgtt tgttgagaat gcaattacag cttttccaat tatttttact tcaactggct
      181 tattatctga aataattact tttactaaca tcttgccttt gcgttttatt gcttctcctt
      241 gaactgcttc aaatataaac tctgtgttgg gtataggtat tactttataa tgtacaaggt
      301 acgcacctaa aggtccatta gcatttcctg taacagggtc ttctgaaata ccaattgcag
      361 gagcaaacat tcttccatga acttgtagag tttctttatt atgtaatgta aaaatataat
      421 atccattaca ttttatttct tgacttattt tcgataattc atataaattt ggagataaac
      481 tatttaatgt tgataattcc tttattccaa tcataacttt tgaatgtcct gtagatgata
      541 tggcaatagg acaatctgta cgaatatctt catttaaaat accaaatgca ttaagtaatc
      601 ttttttgata ggtttgttgt aatggtggag atacttcaat ttttccttgt gtcataatta
      661 tttcataatc attatctttt tttataatat caactggtaa tattccagca ccagtttttt
      721 gaaatactgt tttgttattg aattggtttt ctactgctct aacataatgg gcagcaatag
      781 tagcatgacc acatatagga acttcattag ttggtgtaaa aaatctgata tgtacatcat
      841 aattttcttg atgattttta gccaatttat tgaaaataaa agcagtttat gaattattta
      901 attctcttgt tattttttgc atttgtgatt cagataagcc atcagcattt gtaattacac
      961 cagctggatt acctgtgaat ctttcttttg taaatgagtc aacttgataa agacgaaatt
     1021 ttttattcat attatataat cctttctaaa cattagatta gtttatctac atagaaaact
     1081 tgagataaat tattatttat tattatctgt ttaaataaaa ttaataaaat aacatatgtc
     1141 tatattaaaa tttaaaaata tacatctaaa aaagtagtat tatgtaaggc tctatgctac
     1201 actgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcatactaaa
     1261 atatgtctta ctaattctcc acaggctatc cccgtagttc ctacggttct tacatacgtt
     1321 atttattttg aattgttatt agtcttaatc cttcttttaa tatatttata cttgcattta
     1381 tatctctatc gtgagtttca ttacaactag gacaaatcca ttctcttata cttaagtcct
     1441 tcacttcctc gtttttatac ccacatttat tgcatatttg tgaacttgca aagaatttac
     1501 ctacctttac aatttttcta ccataccaat tagccttgta ctctaattga cgaataaatt
     1561 ctgaccatga tacatctgaa attgagcgag atagtttatg atttttaatc atattcttta
     1621 cttgtaaatc ttctatgcaa ataatatcat tctcttttat taatttagta gatagttttt
     1681 gtagaaaatc ctttctttga ttagctatat gttcttgaag tcttgcaact tttaatcttg
     1741 ctttgtttct atttaaacta ccgatt
//
LOCUS       DAMKPG010000212         1713 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMKPG010000212 DAMKPG010000000
VERSION     DAMKPG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.220"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(416..1123)
                     /locus_tag="PC621_003695"
     CDS             complement(416..1123)
                     /locus_tag="PC621_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF4110782.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          554 a          285 c          149 g          725 t
ORIGIN      
        1 ttttgggtat atatacttaa tcattttgaa atttgattta aagtataata aaatatataa
       61 aaaaatatat aaaatggtac tataaaattt ttaatcttca tcaatacctt ttttgctttc
      121 attgttaaaa ctattttttc tattagttta tttcttataa aacaaacatt tagtattaaa
      181 atatatatca cacaatagta tttgcaattt aactttaaaa aaataaaaat acccaactga
      241 cctaagcaac tcgattgggt ataaataatc taaaaaatca tttttgtata aactctttat
      301 ttaacttaca tttttattta ccttctactt taattcaact tatatttcta tgcttcttat
      361 actctcattc aacttatatt tctacttatc ttatactttt ataaattcat cacaattatt
      421 tagttctcgc tatcaaagaa attgtattaa aatactttcc attctctact tccatcattt
      481 ttttgtcatt tatagcatat gaattatcac aatttatcca atccttccaa gcttcatcat
      541 gacaatttaa agcttcacat ttctctacaa tcacagactc cgattcagac caaagtttat
      601 tccaccatgt aattgaatgg aagttcatat tatcttgcca aaatggaatg agttcatctg
      661 gtatacaatc cacaaaatct tcttttaatc caggcattgc catagcaagt attcccccct
      721 cttttacaag aggtgatata tgattgtcta aaaatccttc tttgtttcca aaatagtgat
      781 aagaatcgat acttatcacc gcatcaaaga aaccttctgc ataaggtagt tcatgtgcct
      841 ctgcctgtat tggaaatatt ttatcatcca atttaaactc tttaaatctt ttatagtttt
      901 cagttggttc tatccataaa tctgttgcaa atacagtagc atcaaattcc tttgctaaaa
      961 atatcgaaga tataccctta ccacaaccta agtctaaaat acgcattccc ttttctagtt
     1021 ttatcttact tgttaattct tccaatattt ttatacaatt aggtcccatc atatttttca
     1081 tgagaaagtc cttatcgtat ttattgcttt ttgtaaatat cattctatct ttcctttccc
     1141 tatcagttat tatttattaa attttatatg tttgattttt tatatgcttc ttatattgag
     1201 caatctcttt tttattcatt ctttaacaga caattttaat gtgtaatttt atccactctg
     1261 tgatagtaac tttaatgaat aatcttatct aatatgtgat aattaacttt gatgggtaat
     1321 tttatctact ctacaataat caccttaatg agtatcttat ctactctatt atagttattt
     1381 tagtgagtaa ttttatctac tccacagtaa tcgctttaat gaataatctt atccactcta
     1441 ttatagttat tttagtgagt aattttatct actctatagt aattgcttta atgagtaatc
     1501 ttatccactc tattatagct atttttatgg gcaattttat ctactctata atagtcactt
     1561 taatgagtaa tcataatttt tatatcattt tagatactta ctctaataag aagtcatttt
     1621 ctaatactta ataagtttta attacttcca ctttaaccta tatactaatt aacttattta
     1681 atttagtgca tatttgacac tttacctttt att
//
LOCUS       DAMKPG010000213         1699 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMKPG010000213 DAMKPG010000000
VERSION     DAMKPG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.221"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(391..780)
                     /locus_tag="PC621_003696"
     CDS             complement(391..780)
                     /locus_tag="PC621_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4110783.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          558 a          298 c          102 g          741 t
ORIGIN      
        1 aatatcttct attaattctt tatttttatt ttttaaatat cttcattaat tcctatttat
       61 tttatcatct taaatacgaa aacactgtgt catatttgca aaatatatat tgtcatctat
      121 attaaagctt cacaatttat atttaaaaat tctctttaat cacactacct ctatttaaaa
      181 agctacctta attatactta taagtaaagg tagcttttta aatttaatta aatagaatat
      241 ccactatcaa tttatattta atttatttag attcacatta tagtatcata atccacttat
      301 aatcatttgt catattactt aagtagacta atatattaca taaatacact tacatacact
      361 ttatattaat gctataagaa atacgtttgc ttatacgtct ttctcctctt cttttagatt
      421 ttttatccac tcttcaataa gtaagatatc atctttgctc aaattaatat tttcatgtaa
      481 tgacaacaat aaacttttta tagaagaatc atgaatattt ctaaaaaaat ctcttgtctc
      541 aactcctatg tattcctttt ctttaattaa aacttcataa tgtgtatatt tatctatctt
      601 ttgagaattt agaaatcttt ttactactaa tctagataaa agagtcaaag ttgtcgtttg
      661 tttccattga tatttttgct ccattcctaa aacaatatct ctagatgtta ctttggaatc
      721 tgatttccat ataaatttca ttatctttaa ttctgattgt ggcaactttt ttatagtcat
      781 gataaaacct tgctactcag aatatagaca agcaatatcc ataataaaat ttttcgatta
      841 aatacatctt atacggattt atataagctc agacttataa gatatttttc aatcttaatc
      901 ctaaatttag ctatttgtac acggtaaata tatataaata atgtcaaaat tctcttgtat
      961 tatattgctt aattttatga gatattttgt actcagctat ctattttgga tacttgtcca
     1021 gtagctctcc tttcttttaa actctttgat tttaaatatt tgtatttcag taaatcctac
     1081 ttcctaaatt ttttatttac tacaacttat tactgaactt cctacttcct aaacttttca
     1141 tttactacaa cttattactg aacttctcac ttactaaatt tcttacttac tataaattat
     1201 aactaaactt ctcacttact aaatttctta cttactataa attactacta aacttcctac
     1261 tttctaaatt tcttacttgc tataaattat tactaaactt cctactttct aaatttctta
     1321 cttactataa attattacta aatttcctac tttctaaatt tcttacttac tacaaattat
     1381 tactaaactt cctactccct aaatttctta cttgctacaa attattacta ctaaacttcc
     1441 tactccctaa atttcttact tgctacaaat tattactaaa cttcctactt cctaaatttc
     1501 ttacttgcta caaattatta ctaaacttcc cactttctaa atttctcgct tactaccact
     1561 tatcactaaa cttcctactt tctaaatttc ttgcttacta ccacttatca ctaaactttt
     1621 tacttcttaa atttcttact tcttaagaat aatgttaatt ttaattttaa gattatagtt
     1681 tttgccttgt ttaattgaa
//
LOCUS       DAMKPG010000214         1699 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMKPG010000214 DAMKPG010000000
VERSION     DAMKPG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.222"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            119..853
                     /locus_tag="PC621_003697"
     CDS             119..853
                     /locus_tag="PC621_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF4110784.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(995..1486)
                     /locus_tag="PC621_003698"
     CDS             complement(995..1486)
                     /locus_tag="PC621_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF4110785.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          641 a          198 c          242 g          618 t
ORIGIN      
        1 attattaaaa aatttgttta tagaaacttt tattatttta tataaaaatg ataaaaactt
       61 atattttgta tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat
      121 gaaacaaaat aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc
      181 tacaaatgga ttagcaggtg ctggagaatg gcatgtttta aaaaaaatgt tacctgattt
      241 caaaggaaaa agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt
      301 tgagaatgga gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc
      361 tagaaacaaa acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa
      421 ttttccaaaa gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc
      481 atttgaagat atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt
      541 ttctgttgaa catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga
      601 tggcaataat ctacactggc cagtagatag atattttgaa gaaggagtac gtaagtcaaa
      661 ttttctagga gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt
      721 aatgaaaaca ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt
      781 agatacaatt cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc
      841 aaaaaaaaga taagtaaata taaataatca ttattgtaga aaaaaattgt agaaaataat
      901 tacactaatg gagatgatta taccagtggt attaattaca ccagtagtaa ggaggcaaaa
      961 gcattcgttg cttttgccat tttttaagtt gcaattatac ttctacgtag tatatttcgt
     1021 ttccattttc ttctataagc tttttcatat cttcaaattt taaaaataca gtagcagtat
     1081 tatcatttgg atgtatacct attttatcca tttttaataa atccttatca aatacaactt
     1141 ttacaacagc ctctttatca tttaatatac caaaaggagt cacagctcct ttagtaagtt
     1201 tcaagtattt ttgaagtctt tcttctgatg caaaactaag aggtttacta tcaatttgtt
     1261 gacgaatatc ttttaaatct gcttttttgt ctttacctaa tacaactaaa aaatgttctt
     1321 ttcctttagc atcacgaaga aataggtttt ttacaacaaa ttccatatgt aatgttttta
     1381 attcttccat ttcttcaatt gtataaactg gtggatgatt tacagcctca taaggtatgc
     1441 ttaactcatc taatttttta tatacttcct gttgttgatt gttcatgatt tcctcccatt
     1501 agaaatttat tttatatata aataatatta tcacagaaaa ctttaaaaga aaacgtttta
     1561 tataatatag acaatagatt atatttttat aaagaagcaa gaatactcaa taaaacttaa
     1621 agaaattaaa caatttctaa aaataccttg aaaatagaac cttatttgcc atttataaat
     1681 tatataaaaa gggattata
//
LOCUS       DAMKPG010000215         1662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMKPG010000215 DAMKPG010000000
VERSION     DAMKPG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.223"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>1662
                     /locus_tag="PC621_003699"
     rRNA            <1..>1662
                     /locus_tag="PC621_003699"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          477 a          347 c          492 g          346 t
ORIGIN      
        1 gggacacgtg aaaccctgtg ggaagcagga gggaccaccc tccaaggcta aatactacct
       61 agtgaccgat agcgtatagt accgtgaggg aaaggtgaaa agaaccccgg gaggggagtg
      121 aaatagaacc tgaaaccctg cacttacaag ctgtggaagc acatttcttg tgtgaccgcg
      181 tactttttgt agaacgggcc aacgagttac gttaagtagc aaggttaagc acttaaggtg
      241 cggagccgta gcgaaagcga gttttaactg agcgttcagt tacttgacgt agacccgaaa
      301 ccgggcgacc tacccatgag caggatgaag cgaaagtaaa atttcgtgga ggtccgaacc
      361 cacgagcgtt gaaaagctcg gggatgactt gtgggtagcg gtgaaattcc aatcgagccc
      421 ggagatagct ggttctcccc gaaatagctt tagggctagc ctcaaggtga gagatacgga
      481 ggtagagcac tgaatgtcct agggggtatt gcacctaccg aagactatca aactccgaat
      541 gccgtcatct tatacttggg agtcagactg tgggtgataa gattcatagt cgaaagggca
      601 acagcccaga tcgtcagcta aggtccctaa atgtaagtta agtggtaaag gatgtgggat
      661 tgcacagaca accaggatgt tggcttagaa gcagccactc attcaaagag tgcgtaatag
      721 ctcactggtc gagtgatcct gcgccgaaga tttccggggc taaaacttac taccgaagct
      781 acggcatcag taatgatggg taggggagct tcccatacgg gttgaagcat gaccgtaagg
      841 acatgtggac agtatgggag tgagaatgtt ggcatgagta gcgagatgtg ggtgagaatc
      901 ccacaggccg taaacccaag gtttccaggg gaaggttcgt ccgccctggg ttagtcggga
      961 cctaagctga ggccgaaagg cgtaggtgat ggacaacagg ttgatattcc tgtactaccg
     1021 ataaccgttt gagagaaggg atgacacagt aggataagct aagcacactg ttggttatgt
     1081 gtgcccaagc attgaggcag tcaaagtagg caaatccgct ttgataatgc tgggatgtga
     1141 tggggagcga aatttagtag cgaagtagct gatttcacac tgtcaagaaa agtctctatc
     1201 gaggttaaag gtacccgtac cgcaaaccga cacaggtggg tgaggagagt atcctaaggc
     1261 cagcgagaga actgttgtta aggaactcgg caaaatgacc ccgtaactta gggataaggg
     1321 gtgccaccat caggtggccg cagagaatag gcccaagcga ctgtttacca aaaacatagg
     1381 tttctgctaa gtcgcaagac gatgtatagg agctgacgcc tgcccggtgc tggaaggtta
     1441 aggggatctg ttagagcaat cgaagcagtg aacttaagcc ccagtaaacg gcggccgtaa
     1501 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1561 cgtaacgatt tgggcactgt ctcaacaaca gactcggtga aattgtaatt ccggtgaaga
     1621 tgccggatac ctgcgacagg acggaaagac cccatggagc tt
//
LOCUS       DAMKPG010000216         1641 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMKPG010000216 DAMKPG010000000
VERSION     DAMKPG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.224"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            175..420
                     /locus_tag="PC621_003700"
     CDS             175..420
                     /locus_tag="PC621_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF4110786.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(637..906)
                     /locus_tag="PC621_003701"
     CDS             complement(637..906)
                     /locus_tag="PC621_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF4110787.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(1118..1420)
                     /locus_tag="PC621_003702"
     CDS             complement(1118..1420)
                     /locus_tag="PC621_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110788.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
BASE COUNT          658 a          244 c          177 g          562 t
ORIGIN      
        1 taaaactata ttatgatgta attaaatgat tcagtacttt agaggtggta aaatatagat
       61 agatgaaaga gcataaataa agtgatataa taaaaaatta aatattaact ataataaaaa
      121 aatatctggt actaagtata atacaaaaat aatatatcag aaagaaggtg aagtatggga
      181 gaactacaaa aaattccagg ggtagggaaa gcaacagaaa aatctttaat catgttagga
      241 tatacaacta ttaaatcact taaagatgca aacccagcac aaatgtatga aaaggaatgt
      301 ttaatgcgtg gacagcacat agacagatgt cagttatatg tatacagatg tgcagtatat
      361 tttgcatcaa ctgaaaatcc agaaccagaa aaattaaagt ggtggtattg gaaagattaa
      421 aaaatcaaaa aagttagata taaaaaagag ttgaatatcc gtttaaataa taaaaactaa
      481 tactcaactc ttttttagtc gtaaatttta tgtaaatcta tttgtatggc taaatatacc
      541 catcaataaa tgtataaata taccaatcaa taaatgacta aacatactac ctaacaaagg
      601 attaaataaa ttacttaaca aagcattcta aaacaattaa ttagaaactg tttcttcaag
      661 ccattttcta cattgcccaa tatttaccat agggtcaaag ccttggtctt ttcccttatt
      721 taaaagcata tcacaaggga aagtatcaca ttcaccacaa tgattaagtt ccttactttc
      781 acagcaagtt ttaaccttac attctccacc ccaaaatggt ttttccatag ttgggcaacc
      841 agtgcaattt acttgttctt tttttgtaca actgtcacaa cagacaccac atcttgattc
      901 aaacatgttc aaacatcctt tccattattt aaaaatatta taaaatgatt ttagatgact
      961 acaaaagtgc aaatagttga ttttaagtca tctaaatgtt aaacataaaa ttattaatac
     1021 tggtaaattt ataaaaacta ttccaaaaat atttttgaat atttcaaaac acaataaatt
     1081 acttcaaatc cctttgaata ttccataaca ctatagatta ctttaaatcc ccttgaatat
     1141 atttcacagc attatagatt gctttaaatc tctttgaata tttcataaca ctatagatta
     1201 ctttaaatct ctttgaatat tctacaagac tatagattag tttaaattcc tttgaatatt
     1261 tcataacatc ataaattagt ttaaatctct ttgaatattc tacaagacta tagattagtt
     1321 taaatccctt tgaatatttc ataacactat agattacttt aaatttcttt gaatattcta
     1381 caagactata gattagttta aatccctttg aatatttcat aacaccatag attactttaa
     1441 atccctttga atattctata acactataaa ttacttttaa cttttatata accataaagt
     1501 ttttatctgg cactttaaat ggagttttta tatcaaattt gccttccatc gaataaccaa
     1561 acttaattta attacacatt ccaagagtca aatattcatt caatatattt gataattcta
     1621 tgttatagcc ataattaaat a
//
LOCUS       DAMKPG010000217         1566 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMKPG010000217 DAMKPG010000000
VERSION     DAMKPG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.225"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            5..1435
                     /locus_tag="PC621_003703"
     CDS             5..1435
                     /locus_tag="PC621_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF4110789.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT          722 a          137 c          290 g          417 t
ORIGIN      
        1 ttttatggat ataaagaata aaattaaatc tatattttta ccagaaaaaa atgactattt
       61 agatgatgaa gaatttgagt atgatataga aaataatgaa gaagatttaa aggaagataa
      121 caaagtacat ttaaatgatt taagcagagt aaaatatgat tacagagagt taaaagacat
      181 agaaaaccaa acagaagaga tgtgtctgct agcagtaaag caggatggta ctataattga
      241 gtttgtaaag gacaagactt ataaagtatg tatggaagca gtaaaacaga catataaagc
      301 tcttaagtat ataacaaatc aaaatgagga tatttgtata gaagcagtaa aacagaacta
      361 cagagctttg tactacataa ataataagac agaaaatgta ctaattgagg ctataaaaaa
      421 tgcatcaact tatgatgtca tggaagtatt taaatttgtg gaagatcaaa cagaagatgt
      481 atgtctagca tttatagaaa gggcatctaa aaatgatgtt gcagagatat taaaaggaat
      541 aaaagagcaa actccagcca tatgtttaga agctgtaaaa aaagatggaa aatctttagc
      601 ttatgtaaag gaacaaagta atagtatatg tttagaagct gtaaaagaaa actacagtgc
      661 actatcttgt gtaaaagagc aaacagaaga aatatgtata gaagcagtaa aacagaatga
      721 ctttgcattg tattatgtaa atgaacagac agaaaaaata tgtatggaag cagtaaaaag
      781 aagttatatg gctctacagt atgtaaataa gcaaacagaa gagatttgtt tggaagcagt
      841 aagaattgat ggaagagcat tacagtacgt aaaagagcaa acagaagaaa tttgcttgga
      901 gtcagttaga caaaatggaa aagtattgca atatgtaaaa aaacagactg aaaatatatg
      961 tatagaagca gtaagaggtt catttgaaga acttgaaata aaagaaatat taagttatgt
     1021 aaagatacca acagaaagaa tatttgtaga agcagtaaag caaaatggaa aaattcttaa
     1081 atatgtagaa aatcagacag aattaatatg tttagaggca gtaagagaaa actataatgc
     1141 tttagcttat gtaaaggaac aaacagaaga gatttgttta gaagcagtta atcaaagtta
     1201 tgaagcgctt aaatatgtga aagaacaaac agaagaagtt tgtctaaaag cagtaaaaca
     1261 agattataga atgttgaaat atgtaaataa tcaaacagaa aaaatatgtt tagaagcagt
     1321 aaagcaaaat tatagagctt tagaatttgt tgataatcaa acagaaaaag tttgtcttga
     1381 agcagtaaaa cagaatagaa aagcactaca gtatgtaaaa caaaaacaaa gttaaaaggc
     1441 taaaatatat agaaaatata tgcaaataaa taaaatttga tattttgtaa taaataaaat
     1501 gggtatctca aaatgaactt tttagtttgt gaggtactct ttttatatga aataaaatat
     1561 attttt
//
LOCUS       DAMKPG010000218         1550 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMKPG010000218 DAMKPG010000000
VERSION     DAMKPG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.226"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            3..476
                     /locus_tag="PC621_003704"
     CDS             3..476
                     /locus_tag="PC621_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110790.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(845..1402)
                     /locus_tag="PC621_003705"
     CDS             complement(845..1402)
                     /locus_tag="PC621_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF4110791.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
BASE COUNT          557 a          207 c          169 g          617 t
ORIGIN      
        1 taatgttcaa agaatactta ataagagaat taaaagaaga tgatattaaa aatgcattag
       61 acttaatctg gaatgtattt gaagaatttg aatctccaga ttattgtgat gaaggaatta
      121 cgacatttca tcaatttata gaatataatt ctattattga aaaaatcaaa gaaaaaacac
      181 tcttcttttg gggttgtttt gactctaaaa aacttattgg agtcttagct attagaaatt
      241 taaatcatat atgtatgtta tttgtagata aaaactatca taaacatggt gttgcaaaaa
      301 aattaatgac tacaggcata caatactgta tttgtaataa atttaattta actgaaataa
      361 ctgtaaattc atcaccttat gctgttgaag tttatcataa attaggattt gaagatattg
      421 ctgttgagca aattgttgac ggtatacgtt ttacacctat gaaattgtta atataataaa
      481 ttaaataaaa agcaaagtct cttaaatatt ttatagttac tttgctttta aaccaagatt
      541 taaaataata ctatcttttt tgttttttaa taatatactt taaaagcctt actaatatga
      601 actataattt attttttgat atcctttttg attaacatat aaattaattc aattattaaa
      661 cataaactat gttgtttctc tttatatctc ttctttgtaa acaatattgg gcatttgaaa
      721 ataaatattc ttaaaatata ctgttctcct gttgattttc ctttggtaca aatgataaaa
      781 ttttaattta catgctccgt aatgaagtgt ttaataaata agttaaaata atttatttac
      841 ctccttaatc atatttttta tgaataccta ataacacagt atcaatagaa tcggctaaac
      901 cttttatagc ttcttcttta ctcaaaagtg atttcccaag ttgcaaagtc gaataaccat
      961 gaagtacact cattattaag tttaatactt cacctgtatt ctttgtattt agattacaag
     1021 aaagaataag tttttctaat aaggatttat attcatcaaa gatttctaat gtttcattat
     1081 ttccatgcca agtagcccac tgaattgttt cataaacacc aggattagaa attataaaat
     1141 taaagtatgc tatacttata cactttatag ctgtatctcc aaaatttcca atagtcgcct
     1201 ttatcatttg actattcatc tctctcattc ctttatgagc tacttcaagt aataaatcat
     1261 ctagactttc tatatgattg tacaaagatg gtgtacgtat tcctagcttt tctgcaataa
     1321 cttttaaaga aactttgtta agcccatctt tattaactat atctgatgta gcttgtataa
     1381 ctatttctct actaacacgc atgattttcc ctctttcatt taaaattaat tactttatta
     1441 gtttagctaa tctaattagt ttcgtcaatt attgtaattt ataaaattct ttatactctt
     1501 ataaaatctt gacagtttaa ttataattta atataatact aattgaatta
//
LOCUS       DAMKPG010000219         1481 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMKPG010000219 DAMKPG010000000
VERSION     DAMKPG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.227"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            47..>1481
                     /locus_tag="PC621_003706"
     CDS             47..>1481
                     /locus_tag="PC621_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF4110792.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          609 a          158 c          257 g          457 t
ORIGIN      
        1 atgtatttat atattaaaaa tttattatga gggggacaat tttattatga aattctataa
       61 aagaatatta acattgactt taagtatttc ttttgtattt gcaaatattc aattagtaga
      121 tgcaatatca tctgtagaaa aaatacaagg aaaaaataag tatgaaatag caggcaaaat
      181 agcagataaa aatgcatata aaactgctat attaattaat actagtaact ctattgcaga
      241 tggattaagt gcaagtggac tagcaggagc tttaaatgcc cctatactat taactgaaaa
      301 aaatactata ccaactgaga catctgcaag attaaaaaat gtatcaaaag tttatataat
      361 tggaggaaca tattctatta gtacatctgt cgaaaattca ttaaaatcta agaaaatgaa
      421 agttgtaaga attaagggaa atgacagaat aaaaacaagt tataatgttg caaaagaaat
      481 aaattcaata aaaaaagtca atactgttat gctaaccaat gcatataaag gagaagcaga
      541 tgctataagt attgcatctg tagcagcaag agataaagca ccaataattc ttactaatgg
      601 acaaagtata cctttttcaa ctagtggttt aaagtcttat gtaataggag gaacagcttc
      661 aatgagtacc actttagtaa acaatactaa atctactaga ctaggaggga gtactcgttt
      721 tgaaactaat aaagcaataa taaataagtt ttataaggat gctagagagt tttatatagc
      781 tggagcttat gaattaacaa atgcacttgt aggatcttct ttatcaaaac atggtccaat
      841 agttttagtt aatgatggta gcaataaaag cattttaaaa aatgctaaga agataacttc
      901 tataggatat atagattcaa atatagtaca acagtgttta aatataacaa atggaattgg
      961 agatattaat actggggttg taaaaaatgt taaacctact acaaagacta taaaagatgg
     1021 aatgtataag gttggaaaag atatatcagc aggtgaatac ttaattactt caaactcagg
     1081 aagttatgct tcttattatg aagttacaag tgatagtaca ggtaatgctg atagtatact
     1141 ttcaaatgat atttttagtg ggactagata tatcacctta aagaatggtc aatatattaa
     1201 aattgaggat tcaacaatga cattagcaaa gtatgctaaa gcccagaagg ccaagaatgg
     1261 taagtttgga aatggtatgt ataaaattgg attagaaatt cctgctggag aatatattat
     1321 aatgtcaaat tcttctgatg catattatga agttagaaat gatagcttag gtaatgcaga
     1381 gggaatagtt actaatgata ctttttcagg aagaagatat ataacggttg aggaaggtca
     1441 atatttaata ttaaatgatt gttatttaat tgaaaatgaa t
//
LOCUS       DAMKPG010000220         1460 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMKPG010000220 DAMKPG010000000
VERSION     DAMKPG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.228"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            72..1310
                     /locus_tag="PC621_003707"
     CDS             72..1310
                     /locus_tag="PC621_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF4110793.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          618 a          135 c          252 g          455 t
ORIGIN      
        1 ctattttgtt atttatataa aaatacttat ctagttacta gcaattatta gtagaaatgg
       61 gggaaataga gatgaataag ggattttaca aattaaattc agtggtattg aactttgata
      121 ttaagaatga tgttaacaag ataagattta atcattatat aatgagaaaa gaaaacatac
      181 aaagacttca aggaactctg ccaaacggac aattttatat gacagtgaga agagctgcta
      241 cagatttaca actttctatt tctactataa gtaggcttgt aaaagagttt ttggatttag
      301 ggataataag attagtgtct aagggtgtaa aaggtaatcg ttctgtatat agttacgtaa
      361 gtatagagtt taatggaagt ttatttgatg atagcaaata tatggaactt gactgtgtat
      421 gtaatgatga atttattgat aatagtggca tagagtttga ttgtgatagc aaagataatt
      481 cttttaatgg agatataaat gtagaaactg tgcattgcat taatgagtat ttctctgatg
      541 atgatgtaga gcataattat aacaatttcc ataataaaga tactataaag gataaagcca
      601 tttctaatat tagcactatc aaaaaatata ttgatgaatc ttttgataca aataaatcag
      661 aaaattctaa cttacaacag gagtcaataa atttcaactt taatgataca tgcaatgaaa
      721 tagataaaag aacgaataat agtatagaaa ataatcaaaa taaaaggcct atgagattga
      781 gtgacatcag tgttttaagt gataaaggta tgacaaataa aaagaattac tttaatcata
      841 atcatggtat acaaaaaagg aataaatctg caacaaagaa aaaagaatcc ttaaataaat
      901 ttaataaaaa aaatatacag caaagcatta taaatatgct aaataaaaaa tcaggtaaga
      961 attttaaaat cgattctcca ataagcataa aacttatcaa tgaaaggcta aaggagggct
     1021 atgtcttaga agatttctat aaagttattg aggtaaaagt ggctaagtgg aaaaatacca
     1081 taatggaaat gtatataaga ccagaaacct tgtttagcta taagtttgaa tcctatgtaa
     1141 atgaaaacat atctatgaaa agtcaaagta atgttcatcc atgtaaagaa ggcaatagta
     1201 atgatagcag taattatata cattctgatt atacaacata tgatggcagt gtgcgtgaga
     1261 gaaaaaaata tgagaatgta agatactgga atcctatatg tgaaatttaa ttagtttaag
     1321 aatgtattta tgataaataa gatattaata gagaatagtg agctatagca aatgatatat
     1381 caatagttgg tagtaagtta tattaagcaa gatattacta tcaggtagtg aattatagtg
     1441 aacgatatat caataattgg
//
LOCUS       DAMKPG010000221         1428 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMKPG010000221 DAMKPG010000000
VERSION     DAMKPG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.229"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            1131..>1428
                     /locus_tag="PC621_003708"
     CDS             1131..>1428
                     /locus_tag="PC621_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110794.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDID"
BASE COUNT          571 a          129 c          206 g          522 t
ORIGIN      
        1 atatctttat ttgtcatgca taattttact tagaacagaa actttatata aaacaattga
       61 aaatatacct attaagataa ttagaaaaga gctctcacag aacaaattta atttgctcat
      121 gcaacagccc catattgata tacatttagg ttgtataatt atacatttca atattacatg
      181 ttcattagga taatagttag taaatgatat gattgagaga atagatataa taaactaatc
      241 tagtcctgta ttgcaatgtt tattaaagaa tatattttta aagatccaag tactgcatct
      301 cttccagaat tgagttggtt aatagttttt tgatgctatg caatttataa taaaaagtat
      361 aatgatttag ttgatgaatc atcagaattt ataccctcaa agattaatca gattgcaata
      421 aaaataatga tatattctat agttataata tctattttct ttgcaacccc tcatacaatg
      481 aatataaata tatcaaactt ggatatgggt ataattctca tttcagtttt atttatacag
      541 tttttatcta aattaatttt gtatgtatat tttgataaaa gggagatatg taactaagtg
      601 gtaatattgg tgaaaacaaa aattaaagaa tatagaaaaa aactccttat gacacaaaat
      661 gaacttgcta aatcagttag ggtaaggtgt gagactattg tacacttgga aaatataatc
      721 cttctttaaa gttagtgata gatattgcta aagtattcaa tactacagta gaaaatttgt
      781 ctgaatttat tgaagaagga tagtggatag attataaata gttataagat ggttaatgaa
      841 atatagttac ttaattatta agtgtgttag aaatatttat atgtaacaca atgtgtagtt
      901 aagattacct tatctcatct aagactttaa tttttcttcc cataaagagg gtatatcaat
      961 tttctagtgt ggtagcccat tttttattga tttaaattta taatatatat taatagatga
     1021 tacttaaaaa ttttataaaa tatctaagtt gtcataaata aaacgatata tctaaccgaa
     1081 tatggtaaaa tttgttataa tatagataag ttagagtaaa aataatttta atgggggaga
     1141 aaatgcagtc agtaatatgt aaaatttttg ataataaatt actaattagt aatgatttaa
     1201 gtgagcagga tgatgtatta tatgaggtaa tggataaatt tatagatgat attgaatata
     1261 tttcaaaatt aggagatgag caaattaaaa gtatgcttat ggaggtaaat aaatttgaag
     1321 aagattcaac tgaatttgaa ctatatatga aaaacttatg ctatttaaag caatcatatt
     1381 ttatagatac taatgataaa agtgaaataa attctataga tatagata
//
LOCUS       DAMKPG010000222         1426 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMKPG010000222 DAMKPG010000000
VERSION     DAMKPG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.230"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     misc_binding    393..656
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            807..1358
                     /locus_tag="PC621_003709"
     CDS             807..1358
                     /locus_tag="PC621_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF4110795.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          581 a          144 c          227 g          474 t
ORIGIN      
        1 ttaataatat gatgtaatta aaatggagta atattctgtt tcaaaggagg catataaata
       61 caggaatgag tattaaatga tggttaatta agtaaattta ttgataaatg tacttaatta
      121 actttgaaga atttaattcc tcatgaatca aaatataaaa ttacatcaac agttattcac
      181 aaaattaata taagaagaat tagagccaaa gagcagacta ttagaccatt aaagtaatta
      241 ttcatagaat tttgtatatg aaaagaaatg tatagtataa tttacttttt ttataaaaaa
      301 tagattacaa aatatatttt ttataaaaag gtattgacat tttttagaaa aaagaatatc
      361 attattttaa caacaaaatt caatacaata aattaaatcc tatgacaagg aagagtagtt
      421 aaatttaggt tcagtataga gagctgagga tggtggaatc ttagcaagaa aaaatttgat
      481 gaatggccct tagaggaata aattgaaatc atagttttga gagtagactt tatcgtcagt
      541 cgcacgttat agcgaataag gtatgtaagt acctgataat gagatgtatg aaaattgaat
      601 tttatattaa tttttatata atttaggtgg caacgcggat aatctccgtc ctattagttt
      661 aggacggagt tttttatttt actgaaaatt taaaagatag aaggtggtga gaaagatgtt
      721 atgaatttca aaagctaaag atgatgatat agtaggttgg caaaaaaata aattgaaaat
      781 taaaaaaata ggaggaagta caaaaaatga aaacaaatac aaaaaaatta actttaaatg
      841 caatactttt agcgatggga ttattaatac atcaaatcac accagctata ggattaccaa
      901 tgaagccaga tgtaccacta gcaatgttat ttgtaatatt ggttttaaat agagatgatt
      961 ataaaacttg tttgatagcg ggtatagtaa caggaatatt tacagcactt acatcaagtt
     1021 ttccgggagg tcaaattcct aatgtaatag ataaaactct tacagcaaat atagtatttt
     1081 tattaatgaa tattagttat aatatgcctt ttataaaaaa attagctaag aaaacacaag
     1141 atactgtagt agttgcaata ataatgccaa taggaactct tgtaagtgga actatctttt
     1201 tattagcagc tcaagctata gttggtcttc caggagcttc atttacagct ttattcttag
     1261 cagtagtatt accagctgtt ttaataaatc tagttgcagg aatattttta ttcaagattg
     1321 ttagtctatc aataagaaga gttagttatc aagcttaact acgaaataat attttacttt
     1381 ataaagcatt ataataacta aataacataa gaataaagta gattat
//
LOCUS       DAMKPG010000223         1352 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMKPG010000223 DAMKPG010000000
VERSION     DAMKPG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.231"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(564..1157)
                     /locus_tag="PC621_003710"
     CDS             complement(564..1157)
                     /locus_tag="PC621_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4110796.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          477 a          190 c          178 g          507 t
ORIGIN      
        1 taataaacta atttaagaaa tatagaaaat taatttaaag aaatacaata cattaatttt
       61 taagtaacat taaattaatg taagtaaaat aatgttcata tgaagattta aaggtgcatt
      121 ttctgaagct aagtatatgt tatgtctcta tatccggtga agtttaatag ctcaagaaaa
      181 tgcactattt atgatgatta ttttgtttta tttccaattt tggaaataac tattctaatt
      241 aaatctaaaa ttataccata aataagaaaa gaaagtatag atgctatgta ccaataaatc
      301 gaaattgacc ctgtaattgt tctacatggg atttgcatgc ctgaatccat aaaaacaaaa
      361 aaactatcaa tggtagaaat aatagacaaa ataggattta atcctattag aataatactt
      421 tttgagtctt gaccaatttg gtggatataa atctcaaata atgcaattaa aaaaaacaaa
      481 atgtaaaaga tttaataatt tttttcatca aaatacctca cctaatataa attctgtttg
      541 cactatttgc ttactataac tatttatctc cattcagtca catcacttaa ttgtaatcta
      601 ggtcttcttg atggttcttc gtcaggataa cctaaagata ttgctgccac taattgacta
      661 tctgtattta accattcaca taattcacga tatgcaaagt atacatcaca aatccaaaga
      721 ctacctagac ctaattccac agctgtaagc gacatattct gaattgcagc tccaattgat
      781 tgcatgtttg ccatttcata gaatcgttct tcaggggata gcttttctaa aggtgattta
      841 cctaatatat ttaaaataaa aattgttact ggagcttgtt tcatgatttc aactgtgtaa
      901 tttgcaccag cgatatgttg acgacttcct ggaagaagcc cattatcatt aatttcattt
      961 tgaatacctt tgctcatggc tttaagcata gattcttttt ccttttcagt gatgacaaca
     1021 aaacgccatg gttgcctgtt tttagatgaa ggtgcattta ttccagcttc aattattttc
     1081 tctatagttt catgtgaaat gctttgattt ttatattttc tgatacttcg tctttttgat
     1141 atagaatcac ttatcatttt aagtaacctc cgtttatttt ttggaaaaat ctatttattt
     1201 tctattttta attattttag cattaatcag aaaaaaagtc attgatttta gtatacaatt
     1261 attaacgatt atattaagga ttgtatcaaa aagtatgtga ttatactaag ggattatatt
     1321 agacattata tttaaaattg tatgaaaggt tg
//
LOCUS       DAMKPG010000224         1336 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMKPG010000224 DAMKPG010000000
VERSION     DAMKPG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.232"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            27..101
                     /locus_tag="PC621_003711"
     tRNA            27..101
                     /locus_tag="PC621_003711"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC621_003712"
     tRNA            116..200
                     /locus_tag="PC621_003712"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="PC621_003713"
     tRNA            209..292
                     /locus_tag="PC621_003713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="PC621_003714"
     tRNA            322..398
                     /locus_tag="PC621_003714"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="PC621_003715"
     tRNA            406..481
                     /locus_tag="PC621_003715"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="PC621_003716"
     tRNA            570..658
                     /locus_tag="PC621_003716"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="PC621_003717"
     tRNA            662..737
                     /locus_tag="PC621_003717"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="PC621_003718"
     tRNA            744..817
                     /locus_tag="PC621_003718"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="PC621_003719"
     tRNA            832..908
                     /locus_tag="PC621_003719"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="PC621_003720"
     tRNA            932..1008
                     /locus_tag="PC621_003720"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="PC621_003721"
     tRNA            1016..1092
                     /locus_tag="PC621_003721"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="PC621_003722"
     tRNA            1101..1176
                     /locus_tag="PC621_003722"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="PC621_003723"
     tRNA            1184..1257
                     /locus_tag="PC621_003723"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="PC621_003724"
     tRNA            1264..1335
                     /locus_tag="PC621_003724"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAMKPG010000225         1313 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMKPG010000225 DAMKPG010000000
VERSION     DAMKPG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.233"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            13..480
                     /locus_tag="PC621_003725"
     CDS             13..480
                     /locus_tag="PC621_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF4110797.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            491..706
                     /locus_tag="PC621_003726"
     CDS             491..706
                     /locus_tag="PC621_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110798.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          478 a          149 c          166 g          520 t
ORIGIN      
        1 agatgatatt ttatgattac aaaatttttt agtaatatat tatcagtatt tctatatttt
       61 ctattatcac ttttaggagt tgctggtata atattcttat gtatgatacc tatgttttta
      121 tatgatttta gttctgacaa agtaatcgct ttaattgcaa gaatactata tttttctgac
      181 tactttatag tcatcttatt cttgatattt ataattaata gtactaagta ttccccattc
      241 atacttgaaa atgtaaaaag gtttaaagta atgggatgtt gtttattgat aaacactata
      301 attgaatgtg taattggtta taactcaaat actagtctta taatgatatt tggagataat
      361 agtggcggta tctcaccact tatggttatt tcttttatat ttgcactaat gtgctttgta
      421 attgcagaaa catttgataa agctataaaa ataaaagaag ataatgattt aacgatatag
      481 aggtgatgtt atggcaatag tagttaacct agatgtaatg atggcaaaaa ggaaaatatc
      541 acttaaagat ttggcagaaa aaatagatat tacaaatgca aatttatcaa ttttaaaaac
      601 tggaaaagca aaagctatta gatttaccac tctaaacgaa atttgcaaag ctttagactg
      661 tcagccaggt gatatacttg aatatgtaga agatgaaaat gattaaaaaa gagtgtttag
      721 aaacactctt ttttattata ctatatgatt tttattcttt tactatatta ttgacattaa
      781 tattaacttt gtttaaagtc ataacaaaat acgttctaag aaagaaactg ttcctttttc
      841 tatttgttat ccattaccta taagaactgt atcacactaa atcatacttt taaattgata
      901 attttgccac agattcctat atacattctt ttctacctaa tatttattta aactgttagc
      961 aaaacatgga actttttcat ttacttatac ctccatttat attattgcat agtttatagc
     1021 tacgtgttat aaattacatt attgatgatt tatctaacat ttgtaatctt ttagatactc
     1081 tttgaagtta caacactgtt atactctaat taagtaaact aaatctgata caagttggtg
     1141 attatatgaa aagaaaactt tcaacctatt ttaaaggata tattttaggt ttatgtatag
     1201 catttttatt atttactttt atattaaaaa aattaattat agaaaatatc attggaataa
     1261 taattggaac tggaattgca tttttactga tttacctatt taataaaaaa aga
//
LOCUS       DAMKPG010000226         1298 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMKPG010000226 DAMKPG010000000
VERSION     DAMKPG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.234"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            330..707
                     /locus_tag="PC621_003727"
     CDS             330..707
                     /locus_tag="PC621_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF4110799.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            812..1187
                     /locus_tag="PC621_003728"
                     /pseudo
     CDS             812..1187
                     /locus_tag="PC621_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          587 a          108 c          178 g          425 t
ORIGIN      
        1 ataaaaacaa aaataaaata tgcttttagt aacttgttaa cataacttat aatttatttt
       61 ataagcatta gcctatatag ttttgtgcta ctaaatttct ttgattctaa cgatttagtg
      121 ctattctaca actaaaataa tacaatagag ctatttattg tttggtagca ttgtttgcaa
      181 attaaacaaa tatatatata tgaatattat tttacgttta aaaaaacaga ctaaatatgt
      241 agaatatttg gtaaaactta cctctagttt ttgtcttaaa aagctcctat tattaattta
      301 agttagaaga attaaggaag tgacataaat tgaaagaaga aatattaata gaaaaattat
      361 taagagcaga attaatagta atggagtatt tatgtaaaaa agacactcta atgcctaaaa
      421 aagaaattgt aaaagaaata aaacagattt atggatggca taaaagtaca ataaaaatac
      481 tactaaaaag gctagttgct aaaaggtatt tggctagata tattataaat tttcaatatc
      541 attataaaat aataatagat aataaagaat attatacttt taagaaaaag gtactaaaat
      601 ctagtaaaag cataaaaata atgcgttcac ttaccactac acataaaaat gtaagtaaag
      661 agaagttaga cctattagat gagtattata gaaatttaaa agaatgatat agtaaaaatc
      721 gaatgtaatt atttacctta attcttttgt ttgtaaaact ttattgatat agaatttaac
      781 atatacataa attggtaaag ggatgtatac tatgaaaatt agtaaattac cagaagcgga
      841 attaaaagtt atgagatata tttgggaatc agaaaaggta ttaatatcaa gagaaatcac
      901 caagtctatg gaacaaaaat atgaatggaa agatacaact atatttacag tattaaaaag
      961 attactaaag aagagagttt ttagaaacgg agaaaataga taagcgtaca cactataatg
     1021 tattagtaaa agaaaaataa tatcagagat tttaaacaaa agaattttta aagaatacac
     1081 ataaaaattc aatattaagt ttaatgtcag cactatgtag taaggatgat gaagtagatg
     1141 gtgatatttt aaatttatta gaagaatatt ttaagaattt aaaataagaa aatgacagct
     1201 agatacataa taaataaaat gaaggtatga aattataact ttttctacat tcattttgtt
     1261 tattattctg aacatgttaa aaaaatgaaa taagtttg
//
LOCUS       DAMKPG010000227         1289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMKPG010000227 DAMKPG010000000
VERSION     DAMKPG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.235"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(246..1127)
                     /locus_tag="PC621_003729"
     CDS             complement(246..1127)
                     /locus_tag="PC621_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF4110800.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT          436 a          248 c          135 g          470 t
ORIGIN      
        1 aaaaaatttc taaaaaacaa taaaattaag ttataaacac caatatattt ctattatttc
       61 aataagtata caattagatt agaatctaac gctagtaata aaaaattact ttcccaaaat
      121 agatataagc ctcattttat cgttcaaata atgaaaatat atttgatttc atgcaaaaag
      181 agtgcctaat gaactaaaaa gttcattttg agacactcat ttctttatat aaatttatat
      241 agtttttaat cgttcgcatt caaccactca gttaagactt ttatatattt ctcattttct
      301 tcaacgaatg gcatatgtct tgaatactca aataactccc acttactatt aggtattcta
      361 tcatacatag tctttgcaat atatggcgaa cataaatcta ttgctccact agttactaga
      421 caaggctctt ttatttcatg taatctatct gtaaattcat atccagaaag agttcctgta
      481 ggagaaaatt cattttgtcc ccatcctaca atatatgctt cagaacctga ttttttaggt
      541 cttctcaggc actctggaga atcttctgtc acttcacctg cacaatacat ttccataaat
      601 ctttctaatg catcattata ttctttatta gagtaatctc cagtatttac tgcatcaagc
      661 aatgcttttt ggtcaacctc actcatatat gatattcttc ttttttgttc tttctcccaa
      721 agttttgcag aagaaagagt agatgaaagg atgtaagact taataccttt tggtttatat
      781 tcaatagcat accaaatagc ctgcatacct ccccaagatt gacctaacag atgaatttca
      841 tcaagtccta gatgttttct tagttcaata agctcctcta tccaagtatc tgcattaaat
      901 aactcaggat gcccttctac aaaggaattt ccacaaccaa tttggtcata cattattact
      961 tgtctaccac tttcagctat cttatccaat acctcaaaat agttgtgtgt agaacctgga
     1021 cctccatgaa gtaatactaa tggcttcttc ccttccgtat tttctcctac tatacggtaa
     1081 tacgttttaa aacctttaaa aggcatatat ccttcagtaa ttttcatatt tatctcctcc
     1141 aatttgtaat tataaacaat aatcaagttc attaacaata ctatttaaaa cctctcatac
     1201 gtatttaatt atgtctttta atctttatac ctacttttat aaatagtata acataaaagt
     1261 taaaaatctc tatactaaac caattataa
//
LOCUS       DAMKPG010000228         1224 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMKPG010000228 DAMKPG010000000
VERSION     DAMKPG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.236"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(94..360)
                     /locus_tag="PC621_003730"
     CDS             complement(94..360)
                     /locus_tag="PC621_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF4110801.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(403..1212)
                     /locus_tag="PC621_003731"
     CDS             complement(403..1212)
                     /locus_tag="PC621_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110802.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT          417 a          185 c          147 g          475 t
ORIGIN      
        1 ctttaaattt aattataaaa actgttgcag aaatttctac aacagtttaa aaaatttacc
       61 ttacaaaaac ttattttata acaagagagt atattataag gctagtacag cccatttttt
      121 gcactgagaa attcttttgc catcatcacc ttgttctttg tcatatgcaa attgattaag
      181 catttcacat ggaaatgtat cacatagacc acaatgtata aggtttttat cttcacaaca
      241 agattttaca ggacattgtt caccccaaaa tggtttctca atatctgtac aacctttaca
      301 attgactttt tccttgtatc cacattcata acataaaatt ccacatcttg attctatcat
      361 tttaagtcac tccttaaata taaattttat tatgattgta tatcaaaatt tatacttttc
      421 attgtaaaaa tcggacaaat tttttatata ggttgttgga ttaacaccac aaatagattt
      481 aaaatcattt ataaaatgag attggtcgta ataaccaata tcttgtgcta aagttgcaaa
      541 tggtctttta ttgttttgta cttcttgtaa tacaaggttt atacgaagta atctaaggta
      601 tgatttaaca ctcatcccaa gagaattatt aaaaattcta tttaaatgtc gttcactata
      661 acaactaatt tgtgaaatgt ttttaacaga cataatagag ttctgttttc tagcattttc
      721 aagaattggt atagtcatat ctactatatt tgatttcaat aagtatgata agaataacat
      781 atctaattgt tctactaatt cctttatagt acttgttctt tcaaaaatgg aattaacctc
      841 taagctaaat aatgtgttaa aatcttcaag tgaaattttt aaatctgtag attctctttg
      901 agaaagacct gtcaaataat atactccacc tgggcgaaac tcaacgaata ctctaaaaag
      961 tacattttct atatcatttt ttacaatagt agtttttgtt gtggctcccc agaatgcgct
     1021 ttctatacct tttttgtcaa atttaaatat catacatcca ctagcatcag gtattaatgt
     1081 aagcttttct ttaatacttg tatctggaat agatattgta tagtgtgcta tatactgtct
     1141 taacaattta tgtggcaaaa tatatgtata ctgtttgcag gtatttaaaa tgttattgat
     1201 tattttattc ataagtatat cctc
//
LOCUS       DAMKPG010000229         1211 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMKPG010000229 DAMKPG010000000
VERSION     DAMKPG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.237"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            392..904
                     /locus_tag="PC621_003732"
     CDS             392..904
                     /locus_tag="PC621_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110803.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          542 a           85 c          209 g          375 t
ORIGIN      
        1 cttattttta gaatattcag taaaataatt ttgatatact ctatatattt tgtatgttaa
       61 aagtaaataa attattgtta aagaatgtaa agacttttaa attttttatc acaggatttt
      121 aagtaaataa gaaggattgg agtaattaaa aatttttatt ttgcaaaaat aagtatttat
      181 ttatatggat aaatttgatt ttaaatctat caatatagat aaaatcttat tatttaactt
      241 caactattcc ttaaaaaagc aacttttaag gaatagttga aacaaaaagt ttggttacat
      301 aatagtaatt ttagttacaa gaatgaaact gtaaaactta ggattttctt tatataattt
      361 cattgagaac atttaattta ggaggtaagg tatgaaagta aaaaaattta aaagtggtat
      421 agtaatggca ttaacagtaa tggcaattgg agcatcattg acaaatgtat cagctatgga
      481 attaaacaag agtaaagagt ttgaaactaa atacaatgaa atgaagaggg attttcaaga
      541 ggattatgga aaagaagaca aatttaatat aacattggat gatgaattta caaaagaaga
      601 agtaaatgga gctaaaggga ttataaagac aactaataaa atgaataatg aggttgtagt
      661 attaaattat tttgatgaaa tgaaatctgg gattgaattt gagttgaagg caaaagcaga
      721 aaaaaatgca attttgagag atgaatttca acaaggttat ggaaaggaag ataaattcaa
      781 agttgtgtta gatgatgaat ttacaaaaga agaggtaaat ggagctaagg gcattataaa
      841 gactacagat aaagagacag gtgaggttat cgtatataat tactttgatg agcttgaaaa
      901 ataatgctta aagattactt tgattgaatg aaaaggtaat tgtgttgaga atattaaatg
      961 actgaataaa aacagttgta tcaaaaacaa acaataatag aatgtaagaa tgattgtgtc
     1021 gaaaatatta aatgactgaa taaaaaatag ttgcgtcaaa aacaaacaat aatagaatgt
     1081 aagaatgatt gtatcaagaa tataaaatga ttcaacaaaa aacgattgtg ttaaaaacaa
     1141 acaacaatag aatgtaagaa tgattgtatc aagaatataa aatgactgaa taaaaacgat
     1201 tgcgtcaaaa a
//
LOCUS       DAMKPG010000230         1110 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMKPG010000230 DAMKPG010000000
VERSION     DAMKPG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.238"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(200..1069)
                     /locus_tag="PC621_003733"
                     /pseudo
     CDS             complement(200..1069)
                     /locus_tag="PC621_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
BASE COUNT          417 a          172 c          107 g          414 t
ORIGIN      
        1 caaaaaataa actaaaaaat atcgaaaaat aatttaaaag acatcaaaac taaatcttct
       61 aatcaacaat taaaagtcta ttttcttagt ttaaaaaata aagatagatt ttttcaaaac
      121 actggataaa taggtaaaaa aataaacagt tagtaaaata aatttttact aactgtttat
      181 ttttattgtt taatatgtcc tataaatcct ctaagtaata ttttccttgc aaaaactctg
      241 attctaaacc tagattttta aaataatcaa ccatgcccat acacgcaaat ttttctgtag
      301 aataatgagt agcacctata acattaatta aattatcttt tgctatttga tggaactcta
      361 atactggttc aaaatgtttt aaaggcttag taaacccagt aatatataga tttataccaa
      421 gttcagcaac ttctcttgct acaaatggat aacttcctcc ccctgctgca atagcaacta
      481 aaccatcttt taatacagaa tctccataat catacaattt cacatcatga ccaacagatt
      541 ttttaattaa ttccttaatc tcttctattg attttagtga tgttttacat ataactccta
      601 cctcaatttt atcgtcatat ttacaaaatg gtttgactat ttctaaaccc atagcttttg
      661 caaaactaac agaagttgaa tagtcactat agttgtctag tggagaatgt aatacataaa
      721 aagaaattct acgatttttc atctcttcca tataagatag tggtatgtcg tgaaatggaa
      781 aaccttcatc tgatgctatg tatcccattg cgtgatgtga aaaaattaaa atatcttttt
      841 catctttatc tataacttgc ttaattattt ctttatcagg aaaagtagtt gtgtatactt
      901 tattaatatt ttgagcatta tctaatacta atcatatgta cttttttcta aattctggag
      961 tgataaaatc attttcaaaa ttcataaaac tccaatcatc attaatattt ttaatattaa
     1021 attctaaatc taatttttta taaagttcaa ttgccttaat aataaccact cctttataat
     1081 aatattgaat tactacatat taatatagaa
//
LOCUS       DAMKPG010000231         1106 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMKPG010000231 DAMKPG010000000
VERSION     DAMKPG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.239"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(272..955)
                     /locus_tag="PC621_003734"
     CDS             complement(272..955)
                     /locus_tag="PC621_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF4110804.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT          430 a          169 c           82 g          425 t
ORIGIN      
        1 aattaattta tatactgctt actaaattta aaacatttta attaatttgt acactgttta
       61 ctatacttga aatacttcta ttaatctata tattgcctac tatacttaag acattttaat
      121 taatttatac actgcttact atacttaaaa tacttctatt aacttatata ttgcttacta
      181 tattttaaat tctttaatta atctatacaa tatttgctat atataaagta tatcaaataa
      241 cagcttcatt tttttgtaaa tttacagaaa gttaattttc attaaatgaa tcatctttat
      301 tgataaacat aattccaaag accactgcca aaataaaaaa acatattagt attgattttt
      361 ccacgaaaat ttttgtaaaa aaagttccta aaatagctcc agatataaag aatataataa
      421 tagcataata ctgtaaactc ttttttatca taaaaatatt cttagtatgt atataatgaa
      481 ataaagtttc tgtagcactt cttaaatttc cagtacacat tgtcgttgca aaactattcc
      541 catttacttt tctaaaactc tctacttgca ttgaacaaat aaaagaaata gcaacattta
      601 caaacatatt aaatcttcct ctaggaataa atgaaacaat aagtattgta atcatttcaa
      661 ttataatgat aacctgacgc caatgaatct ttgtcttatt atttgtaaaa tactttctta
      721 tcttctcggc tataaataca cctaacataa atgccaaaat aggaatcaaa taataaaatg
      781 attcctttac attaccttct gctatattaa gcccaaataa aactatattt ccagtttgtg
      841 cattagcaaa gactttacct cttaccacat aagtataaat atccaaaaat cctcctgtta
      901 ttgccaacaa agaacataaa aagaatgatt cagacatctg actcttgctt gtcatatata
      961 aacctctcgc tttctaatga tagatacaaa atcaaaatca ttttagattc tatttttcat
     1021 tgatataaaa aaattattta tatttaaata ttatctatat ttatacatca tttatattta
     1081 tacattattt gtatttatat atcatt
//
LOCUS       DAMKPG010000232         1096 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMKPG010000232 DAMKPG010000000
VERSION     DAMKPG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.240"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(221..712)
                     /locus_tag="PC621_003735"
     CDS             complement(221..712)
                     /locus_tag="PC621_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF4110805.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          421 a          163 c           93 g          419 t
ORIGIN      
        1 aaataaaaat atctgcaact atatattgca aaaatttaaa actaatttat atatgctaat
       61 ataaatattt ataaagacat aagacaaatt tacctacaga tttaaattta acttatatat
      121 tttaaaaggc taatataata tgtattttct tctatacaat caaaaggctt catatctaag
      181 atatgttatg acactaatat tcacaatttt ttagtaatta tcacattata aaaaatatta
      241 cagctagtcc atcatcagat gcttcttgta taaacttttt aagctcaatt agatttgccc
      301 atacatattc aaaaaaatca tcttcactct catcattcat aacaggataa atattatttt
      361 ctaacatctc attaatatta aatctttttc taaatgcttg ttcttccaaa ctgttcattg
      421 cttttgatag tttttttaca tcatttgctg taatcaacat gacaggaaat tctccatcaa
      481 tttccataag tatgtttcca cttagtacta atttgctaaa tatattctca tcatctccac
      541 caaaaggaca acctgtcaaa gtaaagtgaa tggcatgcca tgctttatca atattgatag
      601 tactattttc gttggcatct tcaaaaacaa aatcttgtaa attttcttct gcttgctgta
      661 atttaaaaac attctcttca cttattttag tataacatcc tatcatcccc ataattacat
      721 tcctcctaag tagcgttttt ttaattgtaa cttataatta aacttagtta caataaaata
      781 tattgagaaa agtattattt catcttaaat ttaaatacct atattatcaa ctaaaattct
      841 gtgtttatag cgtaagtatt attatttttt cctaaaaatt aaatgaatca gaagttctat
      901 aggcaatatt gactataaat aagaactatt acttttaaaa aaaatttaag aaattatact
      961 gtagaatcat gctctactta ttagcttcaa ataaaaaata aatcatgttc tcaaattaaa
     1021 taataaataa caataaattt aaattttaaa ctataagagt ttctgaagtg ttaataagca
     1081 taccaataat tatatg
//
LOCUS       DAMKPG010000233         1034 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMKPG010000233 DAMKPG010000000
VERSION     DAMKPG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.241"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            100..852
                     /gene="cwlD"
                     /locus_tag="PC621_003736"
     CDS             100..852
                     /gene="cwlD"
                     /locus_tag="PC621_003736"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF4110806.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          459 a          107 c          159 g          309 t
ORIGIN      
        1 aataggggca gaaatataat atcattacat aataatatat taataccaat aaataaagat
       61 atatcaatat taaaaaaatt taaatgtttg tactaatata tggaatatta tagtgttttt
      121 ataagtatac atatacaggg tgatgttgtg agaaagtaca taaaacatat aatttttagt
      181 tttgcaatga tatgtctagt agtagtatcc atatttgaaa ttaaaaatat ttctgaagat
      241 gttatcaagt atatgccagt aacaaacaaa actataattt tagatgctgg tcatggaggc
      301 attgatccag gtgcattaaa taaggataag agtacatctg aaaaggatat taacctagca
      361 ataacactta agcttagaga gcttatagaa tcaagtggtg gccttgtaat attaactcga
      421 gaagatgaca gtagccttta taaagaagaa aataataaaa ctacaagaca aaaatataat
      481 gagaatttga aaaatagaaa agaaataata tctaattcta atgccaatat gtttgtctct
      541 atacatctta atgcatttga acaatcaaaa tactatggag ctcaaacttt ttatcctaaa
      601 gacaaacaag acagtaaaga attatcgaag tgtattcaag aagaacttaa aagagtagta
      661 gataagacaa acaacagaga agtcaaacca agggatgata tatatctttt gaaagaaaat
      721 aatattccat cagtactgat agaatgtggt tttttatcaa atgaaaaaga gtgtaaactc
      781 ttaactgatg aaacatatca agaaaaaata gcatgggcaa tctacatagg aatacaaaaa
      841 tatttaagtt gaaataagta taaaaatagg tctaaatatg taaatagctt gtattaaagc
      901 agttaaaatg cattaatata ggctattttt attttgacaa aaaaatattt aaaataaaag
      961 ttaaaaagtt gttgacttag aataatatag atgatattat atatgagtgc ccaaaaggag
     1021 caccaaaata agac
//
LOCUS       DAMKPG010000234          993 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMKPG010000234 DAMKPG010000000
VERSION     DAMKPG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.242"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            186..692
                     /locus_tag="PC621_003737"
     CDS             186..692
                     /locus_tag="PC621_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF4110807.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          395 a           93 c          154 g          351 t
ORIGIN      
        1 atattttatc tatattttac acttataaaa tatgaaaact acttattgcg aaagtaattg
       61 atttgttgta ttataattta catgaattat gtaggacaat caaaagtcag tattataaga
      121 gtacatatat tcaaattttt aacaattaat ttatataatt aaaaaatgaa gggtaggatg
      181 aagtcatggg ttttagagaa gtaaaaattg aggaactaca gtttaatcca tttactaaga
      241 ttggcaaaga atggttactt ataacagctg gtgattcaga aaagtttaac acaatgactg
      301 ctagctgggg tggtgttgga gtctattggg gtaaaaatgt tgttacaact tacataagac
      361 cacagagata tacaaaagaa tttgtagata gtaatgatac atttacagtt gccttctttg
      421 atgaaacgta tagagaggct ttaaatattt gtggtactat ttctggtaga gatataaata
      481 aaatagaaaa agcaggactt acaccatatt ttgtggatga tacggtagct tttgaagaag
      541 ctaatatgat tatagtatgt aaaaagctat atcatggtaa tatgccacca gaaaactttg
      601 atgctaaaga aaatgataaa aaatggtatc cagagaaaga ctatcatact atgtacattt
      661 cagaaattat aaaagttctt gtaaaagaat aattataata aaaaatatat gaaaagaact
      721 tattataagt aaatgactgt gctttcttat ctgatatcta agtagatgct actaactaaa
      781 taaatattaa ttttaaataa atactattat aaaaaagatg aactatattt actatagttc
      841 atctttttat ttatataagc acttatataa gtagagattt atttgaataa aagtatttat
      901 ttgaaatatt tgagcatact ttataagtat ttttatgcta actagaaaca cttaatttat
      961 ggcatttgta tttgatttat tttaatgtaa ata
//
LOCUS       DAMKPG010000235          965 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMKPG010000235 DAMKPG010000000
VERSION     DAMKPG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.243"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            272..739
                     /locus_tag="PC621_003738"
     CDS             272..739
                     /locus_tag="PC621_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF4110808.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          370 a           63 c          163 g          369 t
ORIGIN      
        1 attaaaaaaa catagatatt tcagtaatct aaaatagtct acaaaaataa cagttttagc
       61 aaaattaata agaatacata tagatttaaa cctaaaagta tgaacatgtc tgttttttac
      121 aagattaaaa tttatattat atgataaaat taacttaact atttcaataa gacttataat
      181 ttttttcttt atactagacc ttattgaaac ctttatagtt taattctaat tatatattat
      241 aaataaagta tgtgtatatt aaggaggtaa tatgaagttt ttaaatgttt taatagtagt
      301 tgaagatatt gagaaatcaa aaaagtttta ttatgatatt ttggggttaa aggtaatctg
      361 tgattttgga gaaaatgttg tattagaagg taatatatca ttacaagaaa aaaagctttg
      421 gttagaattt attaataaga gtgacagtga agttaagttc aatggaaatg atgctgagtt
      481 atattttgag gaagataatt ttgatacttt tgttgaaaga ttgagtacaa tgaaaggtat
      541 agattatgtt catctagcta tagagcatag gtggggtcaa agagcaatta gattttatga
      601 tttagatggg catattattg agattggaga aactatgtca tctgtgtgta gacgtttttt
      661 agatagtggt ctttcaatag atgaggtagc taaaagaatg gatgtaactg tcgaatatat
      721 tgaatcagtt ttagaataga tggattatat taaagtaaaa ctatattttg tatagaagta
      781 agggctgtaa gtaaacttgt atggaaacaa atttacttac agtttttgtt tttgtaagat
      841 acagaatata atatttgtta taattttatt tggattttta aaaagtgcat gattttcctt
      901 acatattata atagaattta atatttattg gatgatttaa atttagaaaa atataagtat
      961 tagct
//
LOCUS       DAMKPG010000236          943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMKPG010000236 DAMKPG010000000
VERSION     DAMKPG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.244"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..801
                     /locus_tag="PC621_003739"
     CDS             <1..801
                     /locus_tag="PC621_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF4110809.1"
                     /translation="VPGDVLDYTIEVINAGPSTAQNVTLTDNIPASILSPEYSIDNGV
                     TFQPWNGSLSIGTLGAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPNLNNNTSTS
                     EIDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPENAQNVTLIDNVSDKLKKAV
                     FSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNIAEVTSTTPDSNL
                     NNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDFWC"
BASE COUNT          370 a          123 c          159 g          291 t
ORIGIN      
        1 gtaccaggcg atgtactaga ctatacaatt gaggtgataa atgcaggacc gtctactgct
       61 cagaacgtaa ctttaacaga taatatacca gcttctattt taagtccaga atattcaata
      121 gataatggag tgacttttca accatggaat ggaagtctta gtataggcac tttaggtgct
      181 ggagagataa gaaatatcat aataagggga atagtaagtc aaacagcaat aggtacaatt
      241 ataaatacag caacagtaag ttcaactaca cctgacccaa atctaaataa caatacttca
      301 acatcagaaa tagatataag ttcatcagca gatatttctg taataaaatt agcaaataaa
      361 acagaagcat gtgtagaaga gcaaatagat tttgtaatag tagtatcaaa tgcaggacca
      421 gaaaatgctc aaaatgttac tttaatagac aatgtttcag ataagcttaa gaaagcagta
      481 ttttcacttg atagaggtgt aagtttccaa ccatggactg gaagtcttaa tataggtaca
      541 ctaccagctg gaactttaag agttattcta ttaagaggaa ttataaaatc aacatgtctt
      601 gatagattaa caaatattgc agaagttact tcaacaacac cagattcaaa ccttaacaat
      661 aatatatcaa gagtgcaagt agaaataaaa caatgttgtt gtaatgattg ctgttgtaac
      721 aactgtagta attgttgttg caatgattgt ggttgtaaag attgttgctg taattgttgt
      781 aaaaatgact tttggtgttg attttataaa ataattttgt ataccttaaa ttaaactgaa
      841 ataaaaaata aattcacata tgagtaaaat attttataaa tattaataaa ttttctatct
      901 ttgatatgga ccacattcgt aatactagtt attaaatgaa gtg
//
LOCUS       DAMKPG010000237          920 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMKPG010000237 DAMKPG010000000
VERSION     DAMKPG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.245"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            103..357
                     /locus_tag="PC621_003740"
     CDS             103..357
                     /locus_tag="PC621_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110810.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            402..725
                     /locus_tag="PC621_003741"
     CDS             402..725
                     /locus_tag="PC621_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110811.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT          375 a           74 c          151 g          320 t
ORIGIN      
        1 aattttggaa atagatgtaa atatatgttc tattgtataa taagagtaat ataaaatcta
       61 gtaaaaataa tcaaataaat ttaacttatg agagggataa ctatggaaaa taaggatttt
      121 aaattaacaa tatgtatatt tgcttttgta attggagtag tagtattatt ctgctgtata
      181 ggtttgggtg aatttagtgc agagcatatt attcaacaaa atggtggaat catggatact
      241 gaccaatata atatttatct agaccaatct atttctcaat atagaaattt tggttctata
      301 ttagctctgt taggaggaat agggatattg tttgataaat atatcagaaa atactagaga
      361 ttatgttttg tctaaaataa attcttatga ggagataaaa aatggcaaca gaaatattta
      421 ctgtaaaaat tacaccctta gaagcacaaa aaatgataaa agaaaatatt gatgcagatt
      481 tagtttttag tgattcttat aatttaggag atgataaatt tatactaatt acaacttttg
      541 agaagtatta cgcaagaaat aatagtgatg caggattaat cgtagtttat gaaaatacca
      601 cagaaaaaac agttattaaa gctacttcaa caggcagtgc aattggagtt ttaaaaattg
      661 attggggtgc aggtggaaat ttacttaaaa gagtaaaaaa catacttgct gaatatatta
      721 tttaattatt ttatctaact gataatatgt caagaaaatc tcgtaaaaga ggttaagaat
      781 atttttataa tatatagata aataaggatt tgaaggtaaa gtttttaatg tataaagaaa
      841 aataatataa taaaacgaat tggctttata attctttata aggcaaagag tacatttctt
      901 tatataattg ttggatatta
//
LOCUS       DAMKPG010000238          868 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMKPG010000238 DAMKPG010000000
VERSION     DAMKPG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.246"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(170..868)
                     /locus_tag="PC621_003742"
     CDS             complement(170..868)
                     /locus_tag="PC621_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110812.1"
                     /translation="MCEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYPN
                     NVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKPL
                     KYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGIY
                     TFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIKL
                     LREKLNSCLVDVII"
BASE COUNT          310 a          111 c           65 g          382 t
ORIGIN      
        1 atctttaaaa taaaattgtt aatagaattg taaaataaaa tcttaatata ttcaatctgt
       61 tattgatgaa aggtatattt ttaactataa ttagttttta ttataaattt tttcatattt
      121 tacaatagat atattctatt agaatagaat ataagggcaa aaatttacct caaattatta
      181 catctaccaa acaactattt aacttttctc tcaataattt tatcttataa cgacttacaa
      241 ttattgaatt ttgatttata aatacaatat tattttctat atctagcata tttaccttca
      301 ttaaatttat aaggtagctt ttatgacatc taaaaaaatt atgttcttct agatttgatt
      361 ctatttcttt catactattc ttaaaagtat atattccttt ttcagtatgt atagttaaat
      421 cattttttaa aacttctaaa tataaaatat catctacctt tatgaaatat atcttgcatt
      481 tacattttat agataaaata gcttcatttt tgtagcattc ttttaaatat gatgatattg
      541 cttctttaaa tagagagtat tttaagggct tctctaaata tctataaact cctatctcat
      601 aacctttctt tatgtattta tttgatgagg atacaaatat aattcttaca ctttcatcaa
      661 attttttgat ttcttttgaa acattaaatc cattttcatc atttaagtct atatccataa
      721 atatgatatc aacattatta ggatactttt ttaataaatc gcctccattt tcaaactctt
      781 tgatattgta ttcaaaattg taattataaa aaaaggtatt tatataatca tttagaatta
      841 ccctctctga ttttttacct tcgcaaat
//
LOCUS       DAMKPG010000239          859 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMKPG010000239 DAMKPG010000000
VERSION     DAMKPG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.247"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            311..781
                     /locus_tag="PC621_003743"
     CDS             311..781
                     /locus_tag="PC621_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF4110813.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          399 a           63 c          159 g          238 t
ORIGIN      
        1 aaaaataaag gattaataga agatactgaa gaggtagaaa caaaagtgtt aatagaagat
       61 attgaaaaaa taaaaacaaa agattaataa aaggtactga agagatagag ataaaagtgt
      121 taatagaaga tattgaaaaa taaaaataaa agattgatat aatatactga agaggtagaa
      181 acaaaagtgt taatagaaga tactaaaaaa gtaaaaataa aggactaata aaaggtactg
      241 aagagataga gaaaaaaatg ttaatagaag atactgaaaa aatagaaata aaaaattagg
      301 aggaaaattt atgaaatatc aggtttcatt attagcagtg aaggatattg aagtatcaaa
      361 aaaattttac aaagaattat ttgagcaaga aattgaattg gacttaggca aaaatgtaac
      421 atttaaagga ggatttgcta tacaagaaga cttcgcatgg cttacaggga ttaatccaga
      481 tacaattata caaaaatcaa ataatatgga actttatttt gaggttgatg attttgacga
      541 atttattgaa aaactcaatt attataaaga tgtagagttt gtccataagc cactaaaaca
      601 tgaatggcaa caaagagtag tgcgtattta tgacccagat aagcatatta ttgagatagg
      661 agagtctatg gaagttatag ctagaagata tttatctgag ggatactctg tagaagaaac
      721 ttctaaaata attcaacatc caattgaatt tgtaaagcag tgtgaaaata gtaaggaatg
      781 attgtttaaa ttataattat ttagtagaaa ttaattgtag tggaactaat tcaagataat
      841 atataggact agtatttga
//
LOCUS       DAMKPG010000240          779 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMKPG010000240 DAMKPG010000000
VERSION     DAMKPG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.248"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            54..620
                     /locus_tag="PC621_003744"
     CDS             54..620
                     /locus_tag="PC621_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF4110814.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          299 a           68 c          146 g          266 t
ORIGIN      
        1 actttaatag tgatataata ataaaaaaat ttttaaagag ggtgatatta catatgagaa
       61 aacttgaaga gatattagaa tataataaat catttgtaga gaacaaagaa tatgaacaat
      121 atgtgacatc aaaacatcca aataaaaaaa tagtagtctt atcttgtatg gatacaagat
      181 taactgagct tttgcctaag gctatgaatt tgaaaaatgg agatgtgaaa ctaataaaaa
      241 atgcaggagc tactataatg catccttttg gaagtattat gagaagtgtt ttagttgcaa
      301 tttacgaatt tgatgtagat gaggttatgg ttgtagggca tcatggatgt ggtatgtgta
      361 atgttgatac agataagtta cttggtaaaa ttttagatag aggaatatct aatgatgtaa
      421 tacttacact tcgtagtgct ggaattgata caaaacaatg gttacatgga ttcgattctg
      481 cagaagaatc tgtaaaagat agtattgatt taataaaagg gcatccttta attccagatg
      541 gtattattgt acatggtcta attatatcac ctgaaactgg gaagttagac gttgttgtaa
      601 atggatatga agataagtag agtattgtaa tgttatataa tcaagatact gttgaatatg
      661 atgttgtttc aaaatttatt ttgggacaac ttttttattt gaaaatatat gaaaaggttt
      721 attttaagct ttacaataaa aaatataagt agctgactaa ttaaaaatct tactattat
//
LOCUS       DAMKPG010000241          761 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMKPG010000241 DAMKPG010000000
VERSION     DAMKPG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.249"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            68..>761
                     /locus_tag="PC621_003745"
     CDS             68..>761
                     /locus_tag="PC621_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110815.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLV"
BASE COUNT          281 a          109 c          127 g          244 t
ORIGIN      
        1 tacaacatta ttttatagtt tgattaattc aacaatcact aatatatcaa actgaaagga
       61 gatttttatg gaaaaaaaat atttcgaaat tcatggagtt ccttcaatcc tttggggaaa
      121 tccttcaagt aaattatata tttacataca tggacaatgc ggcaataaag aggaggctga
      181 agcttttgct aatattgcag ttccacatga gtatcaagtt ttaagtgttg atttgcctga
      241 acatggagaa cgtaaaagag aaaaaaattc atttaaccca tggcatgttg ttccagaatt
      301 agtatctatt atggaatatg caaagtgcca ctggaatcaa atttcgttat atgcaaatag
      361 cattggagca tggtttagta tgttaagctt tgaaaaagag aacttaagag agtgtatttt
      421 tgtatctcca attctagata tgcaacagct catttcaaat atgatgcttt gggcaaatgt
      481 atcagaagaa cagcttaaac atgaactaat tatcccaact tcatttgggc atactctttc
      541 atgggaatat ctacaatatg taaaacaaca tcctatcact caatggaatg taccaacaaa
      601 aatattatat ggtaaacatg atgaacttac tgaaatcggt gttgtagaga aatttgtaga
      661 aaagtttaat tgcaacctta ctattctgga aaatggcaaa cattggttta acacaccaca
      721 agaattaaat tcattatatg aatggttaaa tactttagtt a
//
LOCUS       DAMKPG010000242          760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMKPG010000242 DAMKPG010000000
VERSION     DAMKPG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.250"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>760
                     /locus_tag="PC621_003746"
     rRNA            <1..>760
                     /locus_tag="PC621_003746"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          204 a          168 c          239 g          149 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct
//
LOCUS       DAMKPG010000243          752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMKPG010000243 DAMKPG010000000
VERSION     DAMKPG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.251"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="PC621_003747"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="PC621_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF4110816.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAMKPG010000244          729 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMKPG010000244 DAMKPG010000000
VERSION     DAMKPG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.252"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            44..703
                     /locus_tag="PC621_003748"
     CDS             44..703
                     /locus_tag="PC621_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF4110817.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
BASE COUNT          267 a           60 c           90 g          312 t
ORIGIN      
        1 attttataaa aaatataatt taatcatttt tgaggatatt attatgttag atgcatttat
       61 aacaattgtt tcttctatta tagaaatatg gtcatgtaaa aaagtttttg attatactag
      121 tcagtcaaag accgacttaa aaaaactaaa tatgtatttt ctcttttctg tttttcttat
      181 tatttcttct ttatatataa atataggagc taatagtaga atcttgatat gtatatttgt
      241 aatgttcgtt ttctataagt ctaactataa ggttaatata tataaatgtc tggtaatctc
      301 atttttttat tggttaatat taataggtat aaatggatta agtatgttat tagtagcaac
      361 agtaagcaat ttggataata tgaatgtttt acttagtggc agtttatata gatatgaagc
      421 aatattcttg ggaaagataa tgttaggtat attactttat gtttataagg cttcttttaa
      481 aaattttaaa ataagtaaaa aagagatata catatgtatt cctatgatag caaatttatt
      541 gtcatttttt gtactttatg attatattat taatcttgta aaaatctctc ctgaaagaag
      601 aactgctttt ttagtaatag ttttactatt aatattatct aacatttcaa tctttgtatt
      661 aatacatata atacttaaga ttaaaattag gaaaataaga taaattttaa tacgattcat
      721 ttaaattaa
//
LOCUS       DAMKPG010000245          723 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMKPG010000245 DAMKPG010000000
VERSION     DAMKPG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.253"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>723
                     /locus_tag="PC621_003749"
     CDS             <1..>723
                     /locus_tag="PC621_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF4110818.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAMKPG010000246          719 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMKPG010000246 DAMKPG010000000
VERSION     DAMKPG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.254"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(21..689)
                     /locus_tag="PC621_003750"
     CDS             complement(21..689)
                     /locus_tag="PC621_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110819.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
BASE COUNT          228 a          118 c           39 g          334 t
ORIGIN      
        1 agattttttt attagaataa ttaaactttt aatttattgt tatttaatct attttgataa
       61 acattattta ttttattatt atttaaatct ttaaaatcta aacttttaca ttgttcatca
      121 ttttcccata ttcctgcttg tttatttcca tcatttttat ataatacacc cttaccattt
      181 ttcttattat ctaaaaaata tcccttataa atgtcattat tgggatatgt taattttccc
      241 aaaccatttt ttaatccgta tttaaattca cctgtatata ttgaaccatc tctataagta
      301 tattttccta ttccatgcat cttgtcatct ttccataatc cttcatactt ttccttactt
      361 gaaaaaatat atattccaaa tccatttttc tttccatcaa caaactcacc tttatataaa
      421 tcaccattat cgtatttaaa tactttttct tcctttaatt tcttattgtt actcttattt
      481 ttattattat tgttatcctt tatatgtatt tcttctcttc tggcttcaat aaaatctcta
      541 tcatacctaa atccgtttct tgatttaatt ttttgattaa tcttcttctt attaataaat
      601 actctagcta gttgagtaaa tataaataca gatgataagc atataaatac acatagtatt
      661 gttgtaaaat aactagttat attttccata aaaaatcacc ctttaaaaaa tttatttaa
//
LOCUS       DAMKPG010000247          700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMKPG010000247 DAMKPG010000000
VERSION     DAMKPG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.255"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..423
                     /locus_tag="PC621_003751"
     CDS             <1..423
                     /locus_tag="PC621_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110820.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="PC621_003752"
     CDS             417..>700
                     /locus_tag="PC621_003752"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF4110821.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAMKPG010000248          699 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMKPG010000248 DAMKPG010000000
VERSION     DAMKPG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.256"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     regulatory      <1..64
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            147..632
                     /locus_tag="PC621_003753"
     CDS             147..632
                     /locus_tag="PC621_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF4110822.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          308 a           60 c          116 g          215 t
ORIGIN      
        1 atgacgcaaa actataggga ctaaaatctt tatgtaataa aggtcatgtc agccagttgc
       61 caaaaagata ataattcttt ttgttaaaag tatttttaga gttatatatt ttaataactt
      121 aatatttggc aagaggaggg cttaagatga tatctgaatt aatgttaaaa aattttggca
      181 aatctagcat caattttaaa actaaaatta aattgaattt aagagaaaaa ccagacataa
      241 atagtttgaa attaaaaagt ataccagaag gtaaaattgt taagttaaaa tgtgtagatg
      301 gaatatgggc agaagttgag agtaattatg acaaaggctg gttactttat aaatatttag
      361 aaagattaag taatgctaaa aacaatagta atgatacaat aaaaaataga aaaaagaatt
      421 acataggaaa tataagaaca aatggattaa gtttagaact tagaaatgat agaacattag
      481 aatctaaagt tataactaca atccctgatg gttttaaagt tgaagtatgt tatagcgttg
      541 gaaaatgggc aagagttaat atcaataaaa atgaaaaacg atattctgga tatgtatata
      601 atcaatacat agaagagcat ttaagtttat aatacgatac tagtgagctt taaatatgtt
      661 aataaatcac aaaaaagaat cccattaatg ggattcttt
//
LOCUS       DAMKPG010000249          689 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMKPG010000249 DAMKPG010000000
VERSION     DAMKPG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.257"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            97..639
                     /locus_tag="PC621_003754"
     CDS             97..639
                     /locus_tag="PC621_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF4110823.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          286 a           58 c          124 g          221 t
ORIGIN      
        1 tttaccttct tcaattaata tattattatt agtaataact ttaaaattat attactatta
       61 tataaaaatt ggcttaaaag aaggtgaaaa aagtaaatga aaatatgcat aacagtaggt
      121 catagtatac taaaaggtgg taaaagtaca ggtgtaaatg gtatagttga tgaatacaat
      181 tataataaaa aattagctcc tatgttagct gagatgctta taagtcaagg taatacagta
      241 gatgtaataa tttgtccaga aaggcacttt atgtcagaaa gagaagaatt tttctataga
      301 gttccaaaag taaacagtgg taaatacgat ttattagtag agctacattt aaataaagct
      361 gatggaacac aatgtggtac agaagtgctt tactatggca ctgaaggatt agaatatgct
      421 aaaagagtat ctaatagact tggagaatta tttgaaaata gaggagctaa aaaaagagaa
      481 aatttatata tattgagaaa tacaaatcca gttgcaatac agattgaaag ttttttctgt
      541 gataatgtga atgattgtaa caaagcaaat gaatcaggtt atgattatat agcacgattg
      601 attacagaag gtatattgaa taaagatata aatatgtgag attatggtga tttaggtatt
      661 gattaatacc ttttaatatt agatataaa
//
LOCUS       DAMKPG010000250          675 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMKPG010000250 DAMKPG010000000
VERSION     DAMKPG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.258"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            20..>675
                     /locus_tag="PC621_003755"
     CDS             20..>675
                     /locus_tag="PC621_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110824.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     "
BASE COUNT          281 a           71 c          106 g          217 t
ORIGIN      
        1 agatggaggt cgagtaattt tgaaaggaaa catagtacaa tataattttg cagatataga
       61 ggaagaagta tattcgttgg attatgctat agcatggaat acagatgagg aaaacgtaaa
      121 cataatacca tttacaaata aattctgtaa agaatctata gaatcttttt gcctaggtaa
      181 aattaataac tttgttgaaa tattaaatga aggttttgtt gaaaatcatc attatgtaca
      241 tttagataaa atgattagtg ttcctaagaa aaaggttaat ttagtatatc aacaagacac
      301 acatggttat cttctaagag atgataatga caaccttatt cctgcaaaaa taacttcaga
      361 gcaatctaaa tctatatcaa gtaagatgga gttattctgt gctggtgaag aaaaatgttt
      421 aattaatata ttactaaagg ctgacccatc atatatttta gatgtagata gtataaaaga
      481 caaaaacatt ctaaatctag gttatgagtc tattgacaga tataaagaat acaattttga
      541 tgatgataag attttaatat tttttataaa taaaaaacgt tattcagtga ttatgaaaaa
      601 aacaaacaac tctgataacg atttagtttc cagaaacaat gctattaaag aattatttac
      661 taataaagca ggaaa
//
LOCUS       DAMKPG010000251          603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMKPG010000251 DAMKPG010000000
VERSION     DAMKPG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.259"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(97..576)
                     /locus_tag="PC621_003756"
     CDS             complement(97..576)
                     /locus_tag="PC621_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF4110825.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          238 a          102 c           48 g          215 t
ORIGIN      
        1 atagtttatg tattataccc taatatattt tattttaaat tactagttaa tcactctttt
       61 ctggttattt atctcctata tattaaagtg tcacacctaa atagttaaat cattatcctc
      121 ttttatcttt attgccttta caaatccatc tgcaattaca aaactcataa gagaagttat
      181 aaaatataca aatactggaa cagtaaaata aaatccaaat cctaaatcta ggaatctcat
      241 tccttttcca gtagtaccaa aagttgaaat aaactccatt atagagttta aaagcaaaag
      301 atatccaata atcctaaatc tttttacatt atcttttaca aataagctat gttcagaact
      361 acttaatact tctatcaatg attttataat gataaaatat gatacaccta aaataaacat
      421 taatattcca gaaatagtgc cttgtagagt tgcttctcca ctaaaacaga ggtacataaa
      481 acttttaaat cctcccacaa caattactaa ccctaaaaga aaaaataaaa tttgtaatat
      541 accacttagt aaatttttta taagtttatt aggcatcata ccaactcctt taaaatattt
      601 tat
//
LOCUS       DAMKPG010000252          582 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMKPG010000252 DAMKPG010000000
VERSION     DAMKPG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.260"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..161
                     /locus_tag="PC621_003757"
     CDS             <1..161
                     /locus_tag="PC621_003757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110826.1"
                     /translation="KTAFNEETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKL
                     STLLDKSK"
     gene            188..>582
                     /locus_tag="PC621_003758"
     CDS             188..>582
                     /locus_tag="PC621_003758"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF4110827.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIP"
BASE COUNT          252 a           50 c          100 g          180 t
ORIGIN      
        1 gtaaaactgc atttaatgag gaaactagag gcagatttaa aaatattagt ctagaaaatg
       61 ataatagaaa atttaaatct gttgaagaag aattgaagta cttacgcatg gagaatgaat
      121 ttttaaaaaa gttaagtacc ctattggaca agtcaaagta agcgataaat ttaaaacaat
      181 taaacttatg tctaggaagt atagtatttc tagtatgtgt aagttgatag gtgtgtcaag
      241 aagcggttac tataaatggc taagttattc taaaaaatct tccgataggg gtatcaaaga
      301 tagaattatt aaggattata taattgaaat acataaaaaa tatagaggaa cttacggtag
      361 aaaaagaatt tgtacttatt taaataaaat acttgatagc cctattaatc ataagaaagt
      421 atataggttg atgaaagaac taggaattaa atctattatt agaaagaaag tatatagacg
      481 taagtttaaa tcatatgagg tatatgataa tatattaaat cgtgaattta gagctaatca
      541 accattagaa aaaatttgta tggatataac atacatacct at
//
LOCUS       DAMKPG010000253          575 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMKPG010000253 DAMKPG010000000
VERSION     DAMKPG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.261"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            222..>575
                     /locus_tag="PC621_003759"
     CDS             222..>575
                     /locus_tag="PC621_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4110828.1"
                     /translation="MNLQSKWVNFLKPTYVSDEIKRKKLQVIIFFSITYGLSYSLGLL
                     AICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFYSSVILFFIIYFFIFI
                     IESLKLISINQFQLMY"
BASE COUNT          229 a           46 c           67 g          233 t
ORIGIN      
        1 aatgattttt atataaattt taaacagaag aataaatttc taacgaaatt ggatatttct
       61 tctgtaaata catattcaaa aaaagaatta taagagttta gtcagcagtc gtgtaagtaa
      121 taaggacttt ataaatatat agaaatataa tgatatgagt ataagtagaa aaattttatg
      181 atttttacaa ttatttttta taaaaaggga ggttatgttt gttgaattta caatcaaaat
      241 gggtaaactt tttaaagcca acatatgtca gtgatgaaat aaaaagaaaa aaactacaag
      301 ttataatatt tttttctata acttatggtt tatcttattc actaggttta ttagcaatat
      361 gttttaatca tttaatagat tcagaaattt tagctcaatt tatgatgata ttgccattat
      421 cttcagtttc tattgcaaaa ttttacacag aaggaagaaa taatgataaa tataatttct
      481 attcatcagt aatattattt tttattattt acttttttat atttattatt gaatcattaa
      541 agttaataag tattaatcaa tttcaattaa tgtat
//
LOCUS       DAMKPG010000254          568 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMKPG010000254 DAMKPG010000000
VERSION     DAMKPG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.262"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(339..539)
                     /locus_tag="PC621_003760"
     CDS             complement(339..539)
                     /locus_tag="PC621_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF4110829.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          205 a           73 c           60 g          230 t
ORIGIN      
        1 acatatagat atgttttaat tgctttttat taacaaatta ctaaaagtgt tatatcagaa
       61 ttgaatacaa aagaaagagt atgtatttat gagatagttg aaaattttga tgtgccacaa
      121 ctaaaattat tatattacta tatattctaa ttaaaagagt acttgtaggt ttaagtacat
      181 actttataaa tatatttttg tcagatttat tttataaaaa gtatttagac taagtattat
      241 ttttaagtat tttcttgttg aagttatttt ttcctttttt acaaaatgcg taaataaaaa
      301 aaagactctt aaaagagtct ttccgaaaaa tctatatatt ataatatagt tatattttct
      361 gcttgaggac ctctagcgcc tttaactata tcaaagctta ctttttgacc ttcttctaat
      421 gatttaaatc ctgaagtttg tatagctgag aaatgagcaa atacgtcatc tccactttcc
      481 attgatataa atccaaatcc tttttcatta ttaaaccatt ttactattcc attattcatt
      541 aaaaatactc ctattaacct atatcaag
//
LOCUS       DAMKPG010000255          546 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMKPG010000255 DAMKPG010000000
VERSION     DAMKPG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.263"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
BASE COUNT          219 a           43 c           68 g          216 t
ORIGIN      
        1 taaaaataag aatgtataag gtggtaaatt tatataaaaa agaatatgag ttgtaaaata
       61 ttaaataata ttgtactaat tggaataatt ttaactttat tatagattat tttaatgcca
      121 ttagtactga tggtgtattt taaaattggt ataagcatta caaatacgaa tatgccaact
      181 gtaatatgta atataagtag ctatatacat atgtgctgtc ccatatattg tatcattgtt
      241 tatattaaaa agattatgta aattggtatc aatcaataaa tcattttcag aagaaatacc
      301 taaaaactta aaaaatacct atatgtgtat ttagttagat tggagatttt tacatttaat
      361 tttatagtga caatattata ttattgttat gatgtctatt tatatgcaat tactataata
      421 tcagaactta tagtatcatt tgtttcatta tcaataaggt tttttacttt gttttgtcaa
      481 ttttattaaa aaaagttatt gatataaaag ataagaagga taaaactata tgacagattt
      541 caaaca
//
LOCUS       DAMKPG010000256          538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMKPG010000256 DAMKPG010000000
VERSION     DAMKPG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.264"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>538
                     /locus_tag="PC621_003761"
     CDS             <1..>538
                     /locus_tag="PC621_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4110830.1"
                     /translation="YKITSLYIFKNFKIGILLVFYFLFSQIIFGLIISDNQFDYKNFL
                     NYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFT
                     LYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEII
                     INYHSIIQRLIFILLFYTP"
BASE COUNT          176 a           55 c           69 g          238 t
ORIGIN      
        1 cttataaaat aacaagttta tatattttta aaaattttaa gataggtatt ttgctagttt
       61 tttacttttt attttctcaa attatttttg gattaataat aagtgataat caatttgatt
      121 ataagaattt cttgaattat attcttcttc ctataatatc attagtatat atttatcctt
      181 ttttatgtga agagtatggt tggagatctt ttttacaaag tattttattt gatagatttg
      241 gcaaaaaaat aggtattgta atggtaggca cttgttggag tttatggcac ttacctttac
      301 aatttacact atatagtcct caaactccaa taattggagc tatagcccat ttgatatatg
      361 gtattggact ttctatattt ttaggttatg tttatatgaa aactaagaac atatggtttt
      421 gctctataat gcatgttcta attaatagct taggattaat tttcaatgat tctgaaataa
      481 ttattaatta ccatagtatt atacaaagat tgatatttat actattattt tatactcc
//
LOCUS       DAMKPG010000257          503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMKPG010000257 DAMKPG010000000
VERSION     DAMKPG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.265"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            197..313
                     /gene="rrf"
                     /locus_tag="PC621_003762"
     rRNA            197..313
                     /gene="rrf"
                     /locus_tag="PC621_003762"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            319..407
                     /locus_tag="PC621_003763"
     tRNA            319..407
                     /locus_tag="PC621_003763"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:Leu,seq:taa)
     gene            419..495
                     /locus_tag="PC621_003764"
     tRNA            419..495
                     /locus_tag="PC621_003764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Ile,seq:cat)
BASE COUNT          158 a           92 c          111 g          142 t
ORIGIN      
        1 tttgatgtca ttcattaaga tatatgtgca gtttttagag tgctaaatct atatttttaa
       61 acttgtcaaa acttatggat ttttaatagt tttataaggc aaaaaatcga ataaaaaaag
      121 tattgacaca ataagacaaa gatgttatta taatacttgt cttagaaaaa aagttaaatg
      181 aaaatttaga caataatctg gttataatag cagagaggat acacctgttc ccattccgaa
      241 cacagaagtt aagctctcta gcgctgatgg tacttggtgg gaaaccgcct gggagagtag
      301 gacatagcca ggtaatgtgc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      361 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcaccacaa attattgtgg
      421 gccattagct cagttggtta gagcgcccgg ctcataaccg gtaggtctgg ggttcgagtc
      481 cctgatggcc caccacaata att
//
LOCUS       DAMKPG010000258          469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMKPG010000258 DAMKPG010000000
VERSION     DAMKPG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.266"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(34..210)
                     /locus_tag="PC621_003765"
     CDS             complement(34..210)
                     /locus_tag="PC621_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110831.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(264..353)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          178 a           81 c           47 g          163 t
ORIGIN      
        1 aaactaaaaa gggttttcta gaagaaaatc tgtctaatta tacttactta ccaataccat
       61 cacttgatgc aagcaagatt ccacaacttt ttctccgtac tctaatataa aatcatctaa
      121 ctgtgtatct ataaaatcac aataatcata tgaactacca atataagcag ttagcatatg
      181 tgtacattta tccattttat ctctaaacat aatgtattta tccccctaaa aaactttcat
      241 gaaaaacaaa aagaaaatat ctttttggca actggctgac ataacttatt ttataagcgt
      301 tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag ttttattcta
      361 cagattaata atacaataga attagctatt attcaacata tctagctgaa aaattaataa
      421 atttatatat gaacgttaaa ttatttgaaa aaataaaaag cccctctgt
//
LOCUS       DAMKPG010000259          444 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMKPG010000259 DAMKPG010000000
VERSION     DAMKPG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.267"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..362)
                     /locus_tag="PC621_003766"
     rRNA            complement(<1..362)
                     /locus_tag="PC621_003766"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          116 a          108 c           71 g          149 t
ORIGIN      
        1 ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt tatgatatgt
       61 ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg ctcgccgcta
      121 cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag ttccctgagt
      181 tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta agtgagtttc
      241 ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt atcgcagctt
      301 atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt aataacttga
      361 ccagttaaaa aggtttgata gattagaagc tatcaacttc gataatttta aaaagttatc
      421 agctttatat attacaaatt agat
//
LOCUS       DAMKPG010000260          420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMKPG010000260 DAMKPG010000000
VERSION     DAMKPG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.268"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>420
                     /locus_tag="PC621_003767"
     rRNA            <1..>420
                     /locus_tag="PC621_003767"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAMKPG010000261          415 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMKPG010000261 DAMKPG010000000
VERSION     DAMKPG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.269"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     regulatory      158..248
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          175 a           40 c           57 g          143 t
ORIGIN      
        1 taaattaaaa atattttcaa agagtttatt ataaaattgt agactatagt gtttataatc
       61 tttgtaatta ttggtaacta aatttaaaaa ataaaatatt taattataca caaaaaagat
      121 atttaaaaaa tttgttattt attgtatact taagttaaaa ctaaataggc aaatctagag
      181 aaatctagtg acgcaaagct ataggggcta aggtttgtat acacaaacta tgtcagccag
      241 ttgccaaaaa gagttctagg tatattgtat acgtaggaag agtctaggat gactgccttt
      301 ttggtggtca ttttgttatg gaaaaaatca cattatgtag taaaaaacac ctataataac
      361 atattatacc aaatataatt taaaataaag ataaattatc tataaaagtt actat
//
LOCUS       DAMKPG010000262          404 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMKPG010000262 DAMKPG010000000
VERSION     DAMKPG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.270"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            25..>404
                     /locus_tag="PC621_003768"
     CDS             25..>404
                     /locus_tag="PC621_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110832.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNIL
                     TKLVLSIIVIFLVIVLVVLIFIKR"
BASE COUNT          190 a           33 c           57 g          124 t
ORIGIN      
        1 aaaaattttt atgaggggga aaatatgaat aagaagaaaa aattgattgg gatagctata
       61 tcttttctac ttataatcag tattataaat ccattacaag tagaagcaga agctattatt
      121 gagtctagtg tatacatttc ttcaataaaa gagaattata catatgataa tactaaattt
      181 aaaactatta aatctaaaag tagacataaa agtaggaaat ataataaaag taaagtaaag
      241 ttgaaaaaac gaagtataag aactaaaaag tatggatatc aaactataaa taaatataaa
      301 agacgaaatg caagaagaca taatatccta acaaaattag tattgtccat aatagtcata
      361 tttttggtta tagttttagt agtgctaata tttattaaaa gaaa
//
LOCUS       DAMKPG010000263          389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMKPG010000263 DAMKPG010000000
VERSION     DAMKPG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.271"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            28..309
                     /locus_tag="PC621_003769"
     CDS             28..309
                     /locus_tag="PC621_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110833.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          170 a           27 c           65 g          127 t
ORIGIN      
        1 aaaaataaag gagagtgatt taatcgtatg gcatcaaaac aaacaccaag aaacaattta
       61 gaaaagtttt taagaataag agtcttatca catacaggcg ataaaataca tataaagtta
      121 cctgtaaatt ttgcaaaaag aatgattgag aataatgctt tggatttatt taatggaaaa
      181 gatgatgtag tagatggtaa aaaggttact gaaatattag tcaaagcatt tgactatgat
      241 ttaactggag aagtagttca cttggagaga aaaaatggag atattattaa agtttcaatt
      301 gtacaataga attattttat atgaatttta tttttgaata gttataaata attagaaatt
      361 tgtaaaaaag tataatatat tattagtaa
//
LOCUS       DAMKPG010000264          384 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMKPG010000264 DAMKPG010000000
VERSION     DAMKPG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.272"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(114..230)
                     /gene="rrf"
                     /locus_tag="PC621_003770"
     rRNA            complement(114..230)
                     /gene="rrf"
                     /locus_tag="PC621_003770"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(239..312)
                     /locus_tag="PC621_003771"
     tRNA            complement(239..312)
                     /locus_tag="PC621_003771"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(278..280),aa:Gly,seq:tcc)
BASE COUNT          136 a           77 c           57 g          114 t
ORIGIN      
        1 aatatattta attatcttca ctatatcatt tattgccaaa aaaatctata aaagacaaat
       61 aaaaacatat aacaattgta ggtttattaa atttaataca acaaaaaaag attacgtggc
      121 tacgtcctac tctcccaggc agtttcccac caagtaccat cagcactaga gagcttaact
      181 tctgtgttcg gaatgggaac aggtgtatcc tctctgctat tgtaaccaca taatcttttg
      241 gagcgggtga aggggatcga accctcacag ctggcttgga aggccagaac tctaccattg
      301 agctacaccc gcattaaatt tttataaatt agtaaaacta attttttaga gttagcaccc
      361 taaaaactac atatatatcc taat
//
LOCUS       DAMKPG010000265          373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMKPG010000265 DAMKPG010000000
VERSION     DAMKPG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.273"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="PC621_003772"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="PC621_003772"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="PC621_003773"
     tRNA            300..371
                     /locus_tag="PC621_003773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAMKPG010000266          366 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMKPG010000266 DAMKPG010000000
VERSION     DAMKPG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.274"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(<1..278)
                     /locus_tag="PC621_003774"
     CDS             complement(<1..278)
                     /locus_tag="PC621_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110834.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYD"
BASE COUNT          140 a           71 c           20 g          135 t
ORIGIN      
        1 ttatcatatt ttattcttat aagaaaatat aatcctatta ctactaactc tatcactaaa
       61 aatactccat aggtaagaat tgaatcaata ctatctaacc catttctaaa aaaaattaat
      121 gctatacctg ctgctataat ccctatagca ttttttctta ctgcattttg cccaactcca
      181 taaaaacctg gcatttttcc ttttatttcg acagcagtta aaattatccc aaacactata
      241 aataatactc caataagcat cataatcata ctaatcatct aattacctcc ttcacttaag
      301 gaaatttcaa tttctaaaat ctaaaataaa taatatttat tttttataat aatattgtat
      361 atccaa
//
LOCUS       DAMKPG010000267          343 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMKPG010000267 DAMKPG010000000
VERSION     DAMKPG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.275"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            <1..>343
                     /locus_tag="PC621_003775"
     rRNA            <1..>343
                     /locus_tag="PC621_003775"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           81 a           76 c           98 g           88 t
ORIGIN      
        1 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
       61 cccttgtctt tagttgccat cattaagttg ggcactctag agagactgcc agggataacc
      121 tggaggaagg tggggatgac gtcaaatcat catgcccctt atgcttaggg ctacacacgt
      181 gctacaatgg gtagtacaga gggttgccaa gccgtaaggt ggagctaatc ccttaaagct
      241 actctcagtt cggattgtag gctgaaactc gcctacatga agctggagtt actagtaatc
      301 gcagatcaga atgctgcggt gaatgcgttc ccgggtcttg tac
//
LOCUS       DAMKPG010000268          285 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMKPG010000268 DAMKPG010000000
VERSION     DAMKPG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.276"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
BASE COUNT          109 a           24 c           42 g          110 t
ORIGIN      
        1 ttaatataga tagtctaaag tttgattatt aaagatttaa ttgtaagttg ttacagttga
       61 tgatattgat tagagattaa agtttagagt agaaagttac cttaaattta aaaatgcaag
      121 ataacccaat ctaaaaatta taatcactcg ataggtttaa ggatttattt gtttattata
      181 aataggaggg tgtctcaaaa tgaacttttt agtttatgag gcactctctt ttatatagaa
      241 ttaaatatat tttattattt caacaatgaa aaagaagctt atctc
//
LOCUS       DAMKPG010000269          278 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMKPG010000269 DAMKPG010000000
VERSION     DAMKPG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.denovo.277"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
BASE COUNT          101 a           31 c           24 g          122 t
ORIGIN      
        1 ctaaacccag tatttattaa aaatgaaata aaaagaaaaa aaatacaaat tattatcttt
       61 ttctttataa actttggttt aacttattca ttaggtttac tgttatatta taataaattt
      121 attgatccag aaaactttgc atcattcatg atgatattac ctctatcttc agttgctatt
      181 gcaaagtttt atactgaagg attaaataat gatatgtatg aattttattc actaatcata
      241 ttattttttc ttatttattc attattaatt gttcttaa
//
LOCUS       DAMKPG010000270        65117 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.007, whole genome shotgun sequence.
ACCESSION   DAMKPG010000270 DAMKPG010000000
VERSION     DAMKPG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.007"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            659..1471
                     /gene="modA"
                     /locus_tag="PC621_003776"
     CDS             659..1471
                     /gene="modA"
                     /locus_tag="PC621_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110835.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1481..2272
                     /locus_tag="PC621_003777"
     CDS             1481..2272
                     /locus_tag="PC621_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110836.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            2274..2969
                     /locus_tag="PC621_003778"
     CDS             2274..2969
                     /locus_tag="PC621_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4110837.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2956..3687
                     /locus_tag="PC621_003779"
     CDS             2956..3687
                     /locus_tag="PC621_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110838.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            4002..4802
                     /locus_tag="PC621_003780"
     CDS             4002..4802
                     /locus_tag="PC621_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF4110839.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            5343..6059
                     /locus_tag="PC621_003781"
     CDS             5343..6059
                     /locus_tag="PC621_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110840.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            6028..7620
                     /locus_tag="PC621_003782"
     CDS             6028..7620
                     /locus_tag="PC621_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4110841.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INCYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYSEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(7932..9434)
                     /locus_tag="PC621_003783"
     CDS             complement(7932..9434)
                     /locus_tag="PC621_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF4110842.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9544..10209)
                     /locus_tag="PC621_003784"
     CDS             complement(9544..10209)
                     /locus_tag="PC621_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF4110843.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            complement(10248..10694)
                     /gene="rpiB"
                     /locus_tag="PC621_003785"
     CDS             complement(10248..10694)
                     /gene="rpiB"
                     /locus_tag="PC621_003785"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF4110844.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            complement(10869..11789)
                     /locus_tag="PC621_003786"
     CDS             complement(10869..11789)
                     /locus_tag="PC621_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF4110845.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            complement(11792..12604)
                     /locus_tag="PC621_003787"
     CDS             complement(11792..12604)
                     /locus_tag="PC621_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF4110846.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            complement(12696..13727)
                     /locus_tag="PC621_003788"
     CDS             complement(12696..13727)
                     /locus_tag="PC621_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF4110847.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            complement(13727..14779)
                     /locus_tag="PC621_003789"
     CDS             complement(13727..14779)
                     /locus_tag="PC621_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF4110848.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            complement(14909..16090)
                     /locus_tag="PC621_003790"
                     /pseudo
     CDS             complement(14909..16090)
                     /locus_tag="PC621_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430710.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            complement(16138..17649)
                     /locus_tag="PC621_003791"
     CDS             complement(16138..17649)
                     /locus_tag="PC621_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF4110849.1"
                     /translation="MNNRIDAIYARQSVDKKDSISIESQIEFCKYELKGGNCKEYTDK
                     GYSGKNTDRPKFQELVRDIKRGLIAKVVVYKLDRISRSILDFANMMELFQQYNVEFVS
                     STEKFDTSTPMGRAMLNICIVFAQLERETIQKRVTDAYYSRSQRGFKMGGKAPYGFHT
                     EPIKMDGINTKKLVVNPDEAANIRLMFEMYAQPTTSYGDITRYFAEQGILFNGKELIR
                     PTLAQMLRNPVYVQANLDVYEFFKSQGTIIVNDAADFTGMNGCYLYQGRDVKPSKKND
                     LKDQMLVLAPHEGIVPSDIWLTCRKKLMNNMKIQSARKATHTWLAGKIKCGNCGYALM
                     SIFNPSGKQYLRCTKRLDNKSCPGCGKIITSELETVVYQQMIKKLASYKTLTGRKKAA
                     KANPKIAALQVELAHVDGEIEKLVDSLTGANNVLLSYVNVKIAELDGRKQELLARIAE
                     LTVEAISPEQVSQISGYLDTWEDVSFDDKRRVVDLMITTIAATSDSLNITWKI"
     gene            complement(17744..17935)
                     /locus_tag="PC621_003792"
     CDS             complement(17744..17935)
                     /locus_tag="PC621_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110850.1"
                     /translation="MEHIRYKKETEVVTFQGKEITLENLSPVFTPDQEAAKRRELEQQ
                     LYEVFRKYADKRQKEEAGA"
     gene            complement(18005..18949)
                     /locus_tag="PC621_003793"
     CDS             complement(18005..18949)
                     /locus_tag="PC621_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDF4110851.1"
                     /translation="MAQHAILRFEKHKGNPARPLEAHHERQKEQYASNPDIDTSRSKY
                     NFHIVKPEGRYYHFIQNRIEQAGCRTRKDSTRFVDTLITASPEFFKGKSPKEEQEFFQ
                     RAADFLIDRVGRENIVSAVVHMDEKTPHLHLVFVPLTEDNRLCAKEIIGNRANLTKWQ
                     DDFHAYMVEKYPDLERGESASKTGRKHIPTRLFKQAVNLSKQARAIEATLGDITPFNA
                     GKKKEEALSMLKKWFPQMENFSGQLKKYKVTINDLLAENEKLEARAKASEKGKMKDTM
                     ERAKLKSELDNLQRLVDRIPPDILAELKRQQRQHGKER"
     gene            complement(19132..19527)
                     /locus_tag="PC621_003794"
     CDS             complement(19132..19527)
                     /locus_tag="PC621_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDF4110852.1"
                     /translation="MINTIYIHQPEKSVSFTRLPNFLFEAPTFTALPNEAKLLYAFIL
                     RRTELSRKNGWADEYGRIYLYYPIYEVVELLHCGRQKAVNTLRELQYAGLVEIQKQGC
                     GKPNRIYPKSYEAVPNTDFKKSRSGTPED"
     gene            complement(19639..20052)
                     /locus_tag="PC621_003795"
     CDS             complement(19639..20052)
                     /locus_tag="PC621_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDF4110853.1"
                     /translation="MRENPYKRLPPIERKPDGSLYRMTPAQRKQANALIRRECCNYED
                     GNCMPLDDGDTHTCPQTISFSVCCKWFRWSVLPQVGTLEAEIFRDKELKRCAVCGRVF
                     VPKSNRAKYCPGCAARVHRRQKTESERKRRSTVDS"
     gene            20430..20876
                     /locus_tag="PC621_003796"
     CDS             20430..20876
                     /locus_tag="PC621_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110854.1"
                     /translation="MEIIEIKENKKQFLPLLLLADEQESMIDRYIDRGMMYVLVDNGV
                     KCECVVTDEGKGILEIKNIATAPEYQGKGYGKALIDFVAAKYKGKYSILQVGTGDSPL
                     TVPFYEKSGFVRSHSIKNFFIDNYDHPIFEGGVQLTDMIYLQKNIL"
     gene            21176..22654
                     /locus_tag="PC621_003797"
     CDS             21176..22654
                     /locus_tag="PC621_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HDF4110855.1"
                     /translation="MSLINISNLTFSYEGSFHNIFENVSFQIDTDWKLGFTGRNGRGK
                     TTFLKLLMGKYEYSGTISSNVSFEYFPYDVPDADFFVIDVIHEISPNAQDWEIARELS
                     LLNVSDDLLYRSYSTLSKGEQTKALLAALFLKENSFLLIDEPTNHLDTLGRKTLSDYL
                     KRKHGFILVSHDRVFLDNCIDHILVINKTNIEVQRGNFSSWWRNKEMQDSFERAENEK
                     HKKEISRLTAAAQKTSGWSDRVEKSKNGSTNSGSKLDKGYVGHKAAKMMKRSKAIENR
                     QQDLIHEKSKLLKNLETAESLKLSPLPYHTSRLLELSDVSIAYGSRAVCSNISFTIEQ
                     GDRIALQGKNGSGKSSILKLICGENIPYQGILRKSERLKISYVPQSTAGLNGFLSEYI
                     ERNLLDESLFKAILRKFDFPREQFEKRLDEFSEGQKKKVLLAKSLCEQAHLYIWDEPL
                     NYIDVLSRMQIENLLLEYKPTILFVEHDSAFCENIATKTVEL"
     gene            22672..23706
                     /gene="cfr(E)"
                     /locus_tag="PC621_003798"
     CDS             22672..23706
                     /gene="cfr(E)"
                     /locus_tag="PC621_003798"
                     /EC_number="2.1.1.224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105119688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(8))-methyltransferase
                     Cfr(E)"
                     /protein_id="HDF4110856.1"
                     /translation="MIYSKYEVMKRCISGMNFPDYRYEQLIKMIFAQHIPDFHSMYML
                     PERLRSNLAETFGTSVCGLVPVTHRASGQADKVLFQLKDGNCVETVNLHYKKGWESFC
                     VSSQCGCGFGCKFCATGAIGHKRNMTADEITDQILYFYLAGHQINSVSFMGMGEPFAN
                     PNLFDALKILTNPSLFGLSQRRITISTIGMIPGIKRLTRDFPQVNLAFSLHSPFEKQR
                     SELMPINQTYPLHKVMDALDAHVANTKRRLFLAYIMLGGINDSAEHAKALAHLILSRG
                     ALSYLYHVDLIPYNATDKTARKFTASNHEAIKNFSDILHSNRISVAIRMQFGSDIGAG
                     CGQLYADERD"
     gene            23858..24658
                     /locus_tag="PC621_003799"
     CDS             23858..24658
                     /locus_tag="PC621_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110857.1"
                     /translation="MELSIQERLKDLRVERGLTLEQLAEETHLSKSALGSYEGDNLKD
                     ISHHALIQLAKVYEVTVDYLLGRSKTKNHPNADLADLRLSDDMIELLKSGRVDTSLLC
                     ELAVHPDFPRLMADLEIYVNGTAVKQVQSANAIVDIMSAAIMKQHNPGLSDPQLRQLI
                     AAHIDDDSFCRYVIQQDINKIALDLREAHKDDFFSVPEDNPLEDFLQTAEETAKEGSD
                     PEQASLAFICKRLKLNLKKLSEEEKKWLKKIAQKSDLLKNPNPQRGRK"
     gene            24685..25017
                     /locus_tag="PC621_003800"
     CDS             24685..25017
                     /locus_tag="PC621_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110858.1"
                     /translation="MQEAIIKLKLLGQMPDAVKDDPTEETINMYDELLSNVKTPLTRE
                     EVGVLIDIFPEGGMYGVEWDLLKLVESYLIEAPSSEEYRKLITACPSEEWRETMQARL
                     DNWENNKQ"
     gene            complement(25745..26020)
                     /locus_tag="PC621_003801"
     CDS             complement(25745..26020)
                     /locus_tag="PC621_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4110859.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            complement(26081..26539)
                     /locus_tag="PC621_003802"
     CDS             complement(26081..26539)
                     /locus_tag="PC621_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4110860.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            complement(27128..30052)
                     /locus_tag="PC621_003803"
     CDS             complement(27128..30052)
                     /locus_tag="PC621_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4110861.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            complement(30204..30854)
                     /gene="fsa"
                     /locus_tag="PC621_003804"
     CDS             complement(30204..30854)
                     /gene="fsa"
                     /locus_tag="PC621_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF4110862.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            complement(30918..31490)
                     /locus_tag="PC621_003805"
     CDS             complement(30918..31490)
                     /locus_tag="PC621_003805"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF4110863.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     regulatory      complement(31673..31774)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(31880..33031)
                     /locus_tag="PC621_003806"
     CDS             complement(31880..33031)
                     /locus_tag="PC621_003806"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF4110864.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
     gene            complement(33050..33472)
                     /locus_tag="PC621_003807"
     CDS             complement(33050..33472)
                     /locus_tag="PC621_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF4110865.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            complement(33502..35631)
                     /locus_tag="PC621_003808"
     CDS             complement(33502..35631)
                     /locus_tag="PC621_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF4110866.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            complement(35666..37090)
                     /locus_tag="PC621_003809"
     CDS             complement(35666..37090)
                     /locus_tag="PC621_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF4110867.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            37686..39185
                     /locus_tag="PC621_003810"
     CDS             37686..39185
                     /locus_tag="PC621_003810"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF4110868.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            complement(39270..40427)
                     /locus_tag="PC621_003811"
     CDS             complement(39270..40427)
                     /locus_tag="PC621_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF4110869.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(40542..41462)
                     /locus_tag="PC621_003812"
     CDS             complement(40542..41462)
                     /locus_tag="PC621_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF4110870.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            complement(41749..42867)
                     /locus_tag="PC621_003813"
     CDS             complement(41749..42867)
                     /locus_tag="PC621_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF4110871.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            complement(42887..44194)
                     /locus_tag="PC621_003814"
     CDS             complement(42887..44194)
                     /locus_tag="PC621_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF4110872.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            complement(44218..44493)
                     /locus_tag="PC621_003815"
     CDS             complement(44218..44493)
                     /locus_tag="PC621_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110873.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            complement(44628..46124)
                     /gene="abfD"
                     /locus_tag="PC621_003816"
     CDS             complement(44628..46124)
                     /gene="abfD"
                     /locus_tag="PC621_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF4110874.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            complement(46317..47708)
                     /locus_tag="PC621_003817"
     CDS             complement(46317..47708)
                     /locus_tag="PC621_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF4110875.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            complement(47739..49283)
                     /locus_tag="PC621_003818"
     CDS             complement(47739..49283)
                     /locus_tag="PC621_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF4110876.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            complement(49310..50713)
                     /locus_tag="PC621_003819"
     CDS             complement(49310..50713)
                     /locus_tag="PC621_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110877.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            51130..52002
                     /locus_tag="PC621_003820"
     CDS             51130..52002
                     /locus_tag="PC621_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF4110878.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            complement(52189..52788)
                     /locus_tag="PC621_003821"
     CDS             complement(52189..52788)
                     /locus_tag="PC621_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110879.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(52901..53980)
                     /locus_tag="PC621_003822"
     CDS             complement(52901..53980)
                     /locus_tag="PC621_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF4110880.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            complement(54290..55435)
                     /locus_tag="PC621_003823"
     CDS             complement(54290..55435)
                     /locus_tag="PC621_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF4110881.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            complement(55446..56978)
                     /locus_tag="PC621_003824"
     CDS             complement(55446..56978)
                     /locus_tag="PC621_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF4110882.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            complement(57235..58545)
                     /gene="grdB"
                     /locus_tag="PC621_003825"
     CDS             complement(57235..58545)
                     /gene="grdB"
                     /locus_tag="PC621_003825"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(57496..57498),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF4110883.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            complement(58577..59050)
                     /locus_tag="PC621_003826"
     CDS             complement(58577..59050)
                     /locus_tag="PC621_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(58916..58918),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF4110884.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            complement(59162..60448)
                     /locus_tag="PC621_003827"
     CDS             complement(59162..60448)
                     /locus_tag="PC621_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF4110885.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            complement(60717..61034)
                     /locus_tag="PC621_003828"
     CDS             complement(60717..61034)
                     /locus_tag="PC621_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF4110886.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            complement(61119..62066)
                     /gene="trxB"
                     /locus_tag="PC621_003829"
     CDS             complement(61119..62066)
                     /gene="trxB"
                     /locus_tag="PC621_003829"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF4110887.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            complement(62162..62533)
                     /locus_tag="PC621_003830"
     CDS             complement(62162..62533)
                     /locus_tag="PC621_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF4110888.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     regulatory      complement(62637..62747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(63079..64773)
                     /locus_tag="PC621_003831"
     CDS             complement(63079..64773)
                     /locus_tag="PC621_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF4110889.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
BASE COUNT        20028 a        12178 c         8549 g        24362 t
ORIGIN      
        1 gcagaaaata taactatatt ataatatata gatttttcgg aaagactctt ttaagagtct
       61 tttttttatt tacgcatttt gtaaaaaagg aaaaaataac ttcaacaaga aaatacttaa
      121 aaataatact tagtctaaat actttttata aaataaatct gacaaaaata tatttataaa
      181 gtatgtactt aaacctacaa gtactctttt aattagaata tatagtaata taataatttt
      241 agttgtggca catcaaaatt ttcaactatc tcataaatac atactctttc ttttgtattc
      301 aattctgata taacactttt agtaatttgt taataaaaag caattaaaac atatctatat
      361 gtttcaatgt tctatttttt cactgaaata taataaatat aaaattagtt aaaaagtaaa
      421 aaatctacaa taagttaatt tgatttaagt ttttaaattt aaaatacttt aaagtattaa
      481 taaaaattaa aaataaaata actacctatg gaaatgacat tataaataga ttttttctta
      541 actatacata ataaaacata acaaaacatt ataaaacata acaaaaactt agacatcgtt
      601 ttcttagtat gatatactaa aaaagaattt acactaattt actaaggggg aatatcttat
      661 gtttaaaaaa ctttcagtac tagctttagc tatgattatt tcattaagta ttactgcatg
      721 taattctaaa gaaaacaaag ctaatgatga atctaatact aaagtgacaa cagctgatga
      781 ttcaaaagaa aaagagactc tttttgtcta ttcaggggca ggtttaaaga aaccaatgga
      841 tgaaatagca aaaaaatttg agaaagaaaa taatgtcaaa gttgaatata gctatgcagg
      901 ttctgcacag ctaatagctc aaattgagac aagtcacaaa ggagacgtat ttattgttgg
      961 ttctgaacca atttattcaa aggcctttga aaaagaactt gtaggtgaac ataaaacggt
     1021 tgcacatcat acacctgcaa tagttgtacc taaaggaaat ccagctaata ttcaaaaatt
     1081 ggaagactta aataaagatg gagttaaact tattttggga gacaaagagt ctaatgctat
     1141 aggtaagact acacaaaaaa tacttgaaaa aaataattta aaatctataa atgataatgt
     1201 tgtctctaca gctgcaactg taaatgaaat gattgtacag ttaacatcaa gtaaagctga
     1261 tgctacaata gccacaaaag attcggtctt tggcaatgaa gatgtagaag ttatagaaat
     1321 tgcccaagat aaaaatatag accaactcat atcagcagga atcgtaaact atagtgataa
     1381 aaaagattta gctaataaat ttataaatta tatagagtca gatgaagcta aacagatatt
     1441 taaaaaatat ggttttgaac ctgttaagta gtgattaaac ttgaaaaata aattttttac
     1501 tacactactt actggatttt ttgctatagt cttttttttt attgtatcac cacttcttat
     1561 gttaatcatt aaagggataa gttatgttcc tatatgctta aaatccgtgg aagtacagta
     1621 tgcaataaca cttagtatta aaacatcttt aatatctact atagtatgcc ttttattagc
     1681 tattccagta gcatattttt tacatattac taaattacca tttaaaaaac tgattataca
     1741 gataataaat ctccctatgt ctcttccaca cttagtatca ggaattgctc ttttactttt
     1801 gtttggaaga atggggattg gagattccat ttataagatt tttaagcttg attttatatt
     1861 tacaaagcaa ggtatagttt tagcacaagt atttgtaaat cttccactta ctattaaaat
     1921 acttcacact tcactaaatg aaagtaatga aaagatgata tttgttgcac gtacccttgg
     1981 atgtaatagt tgggaagcat ttagatttat aattcttcca aatctaaaaa ctggaatcat
     2041 atctgcaact gtaatgactt ggtcaagagc acttggtgaa tttggtgcag tagctatgat
     2101 agcaggttct acacgtatga aaactgaaat tattcctaca tctatatatt taaatatgtc
     2161 tactggtgat atagacattg caatcggaat agctgtaatt ttgatattta tctcacttac
     2221 atgcttgatg ctttttgaga tattttttaa tagagaggtt gataagaatt aatatgttaa
     2281 aaataaaaaa tttatctctt gatttaccta attttaaatt acaagaaaat agtttaaata
     2341 ttaataatca tgaatacttt gttttattag gagctagtgg gtcaggtaaa actctattcc
     2401 tagaaacatt agcaggaaga tatccatctg ctaaaggaaa tgtatcatac aaagaaaatt
     2461 taatatcaaa cttacctcca gaaaatagaa atataggatt tgtatatcaa aattttgaat
     2521 tatttccaaa tatgaatgtt gaagataata taaggtttcc acttaaatta agaaaaatac
     2581 ctatagatga acagattctt aaggctaacc acttgtctaa attaatgcag atagaagata
     2641 taaaaaatag gtatcctaaa aatttaagtg gaggcgaaaa gcaacgagtt gcttttgcac
     2701 gtgccttaat agctgaacct gatatattac ttttagatga acctatgagt tcattggatt
     2761 atattacaaa aaaacatata agcaaaattt tgaaggatgt ctacataaaa tacaaaccaa
     2821 cagtgattca tgttactcat gatatttctg aagcaatgtt ttttgctcat aagataggaa
     2881 taatgaacaa tggaaaaata aatcatattt ttgatttaaa tgaagaaata aaaaataaag
     2941 gagaatcttt tttctatgaa tatatttgat attttgagag attatctatt agtacaagtc
     3001 gataaatata atttaaatat ggatatgata agtattgtgt ccaaatctct atcttcaaaa
     3061 gaagcaattg gaaatactaa gcgtaaagat ttcccaatta tagttggtaa agaaataatg
     3121 cttgaagctg actttaaagg agctaaagga caagcattta cttctactcc atctacattt
     3181 gaaggttctt taaaagacat attatcatta gatttacatg acaatcctca tgatagaagt
     3241 ctttttatag catcattaaa tgcagttatg aaatacttag gcaagacgga tagaaccatt
     3301 cattgtaaaa ataacgagcc agaggtatgt gcaaaaaaat tccctgagtt tataaaaatg
     3361 gaatttggta atccaaaagt agcaattata ggatatcaac cagccataat tgataatatt
     3421 aaggactttt tcgaaacaag agtattagat ttaaatcctg aatttgtgga tactattcaa
     3481 tataatgtta agattgaaga tggtatacga gattatgaag atgtaatatc ttgggctgat
     3541 ttagtgattt gcacaggaag tacattatgt aataactcaa ttataaattt tctatcactt
     3601 aacaaaccag tttactatta tggaactacg atagctggtg catcaaatat tttaggttta
     3661 aaaagactct gtttttgtag taaataagca atcatatcat caaggagtac acaaatatat
     3721 atgtgtactc tttaaattta aaaagttatt acttttattc taagtactaa agtaaaattc
     3781 tattaaaatg aatcccgaat aaatgtacaa tccctttaaa tctcaattcc tatcaattat
     3841 taatattttg attagtctta tacataaatg ttttctttat tttaaactaa taaaaaaaat
     3901 tttattccat taatctactc tatatttttc agcatttaag aaataatttt tagtactttg
     3961 tttgataata taactaagac aacttttcag gagatgatta aatgacattt aagaataaaa
     4021 aatttactat aaataaatat gaaattctat ctattattct agttttatgc ttgattttaa
     4081 ccggttttag tagctatgtg aattatagtc ataaaaaatt taaagattat gttgtcttct
     4141 atgttcagca tcaagatgat gaagtgttgt gggctgggag tgctattgtt aatgctataa
     4201 aagaaagagg aaaaaatcat gtatttgtag ttttagtatc tactggatgt ggtatatccg
     4261 tctttgataa atataaaaaa tatgaaaacc taacaaatat acaaaaatca gaatatagaa
     4321 atagagaatt tttagcttct cttaatagcc ttggtgtaat aagggaaaat ataatattat
     4381 taccagaaac aaatacaaat ggtaatactg acttcaatta catggaaaaa attgctctta
     4441 catttgaaga aaaatttaaa aatgtaactc atatagcaca tacttataag ttagatgacc
     4501 atttacaaca tttaaaaaat ggttctgtta tccaaaattt atataatgtt ggtaaaataa
     4561 aagatgctaa atattttgta aaacctaaat ttgctgataa aataactttt aataataaga
     4621 tagtttataa agctcattct actgaagact atgaaaaggt taaaaatgcc tgtcaacaat
     4681 acaaaattgt tgatgaatta gaatatagag aaggtattgg ctataagtct gaccataaat
     4741 catttgataa gttattaaat aataaagatg ttcctgctat acttcataca cctaatttat
     4801 aagaaatatt tattctttta atttaagtta taagaaggaa attatttgag attttgtgat
     4861 atagcacttt ttgtgctata ctactttttc tcattcaact attacccata tctaatttca
     4921 tcatctctta aaatatataa agatatcatc attaaaacct ataagcctta ttaaatactt
     4981 tatacatatc tacattaaac tacctattta tattaaacta cattaaaata aatatttgtt
     5041 ataaattctt aataattatg aaactcattt gttatttata tttgttaata tatgtttaaa
     5101 cctaaaaaat taataaaccc aaaataacat aaggagtgtg atagattacc ctagatattt
     5161 tcataaactt ttaccttcag attaatatgg gcgtctatac ttttaaattt actatatgca
     5221 ttgacaatat attaaaagta ctagttttta tttcataatg aacgggctta ctgttttcta
     5281 atacctcttt ttggtataaa ataaaaattg gttaaaaata aaatttcaag ggggttaata
     5341 aattgaatca aaatatattg gttgtagatg atgatagaga gatagttgat gctatagagt
     5401 tctatttgag acctgataac tttaatgttt taaaggcata tgatggactt gaagcattgg
     5461 acatactaat cgaaaatgat attaagttaa taataatgga tgtaatgatg cccaatttag
     5521 atgggcttaa aactacactt aaaataagag aaaaaaggaa tataccaata attctcttat
     5581 ctgcaaaatc agaagatatg gacaaaatta taggtcttaa tatgggtgct gatgactata
     5641 taactaaacc atttaatcca ctagaactta ctgctcgagt aaaatctcaa cttagaaggt
     5701 atataaattt aggtgatttt agtaatacaa ataactcaaa tgatactaat tatattctaa
     5761 aaagtggtgg cctagagcta aatacagata caaaaatagt ttctttagat ggtgaggaaa
     5821 ttaaaatgac accacttgaa tataaaatac ttacacttct tatgtcacga aaaggtaaaa
     5881 ttttctcttc ccgtgaaatt tatgaaaatg tctggaatga agatgcttat agttgtgaga
     5941 gaactattgc tgttcatatt agaagaatta gagaaaaaat tgaaattaat ccaaaagaac
     6001 caaaatattt aaaggtggtg tggggcattg gttacaaaat tgaaaaactt aattattaat
     6061 gattctaaag caaaatttat tttattatta ttactgtttc tattagtttc tatattattt
     6121 gaagcagttt atgatttgaa taataacttt tcttataata ttaattgtta ttataaagag
     6181 tttgctctcc cttattttta ttttttcggt actcctgtac atttgtcaaa agtagaatta
     6241 gagtttgtct gcataggtat tatattatca agttttttat tgactatact atgttttaca
     6301 ttgtataaaa aaaataaaca gactaaagtc aaattcttag agaatgtcat aagccttttt
     6361 tctaaggtaa aaatagaact tacacttata ttaactttga tactattaac cttaaggttt
     6421 catgcattta caagtaataa aatgttttac aacactaatt taataatcta caattttata
     6481 attatatcta tagtctatat aatatatgaa aatattaaat ataataacta taatctaaaa
     6541 gatatgtcta taacatatgt attcttttca aacttactta gtatgtataa taaaaaaact
     6601 ataaataaaa aattagctat agtattttta atatctatag ttgtacaagc tattatccaa
     6661 tatcttgttt tgatattctt tatgtatata tattattctg aggcaatagc tatagtaatt
     6721 ttatttacac ttgtaaacac agtttttcat ttttatatat acaacttttt atctgttaag
     6781 tttaattaca taaataacat atcaaaaaat ataaaaaata tagaaaatgg caatttaaag
     6841 tataaattag aagttatagg agatgatgaa atatctactt tagccaaaag tataaataat
     6901 ataacaggtg gtcttgaaac tgctgtagat gctaatataa aaaatgaaag gatgaaggca
     6961 gagcttatca caaacgtatc tcatgactta aaaactccac ttacatctat attaagctat
     7021 gttgatatgt taaaaaataa tgactttgac agagagacta taaatgacta tataaatatt
     7081 ttagataaaa aatcacaaag attaaaatta ttaatagatg acatatttga agcatctaag
     7141 ctaagtagtg gagatgtaga atttaatata acaaaaaccg atataagaga acttttaatt
     7201 caaagtattg tagagtttga agacaaaatt caaaatagca gtttagattt tataatagaa
     7261 acacctgaat atgctgtttt taccatgata gatggtaaaa aaacttggag agtttttgag
     7321 aatctaattt gcaatatact aaaatactct atgcctaaca ctagggtcta tatagatatg
     7381 tttataaagg aagaaaatat aatattaacg tttaaaaata tttcaaatga taaactaaat
     7441 ctaaagccag aagagttaat agaaagattt agaaggggtg atatatctag aaaaacagat
     7501 ggctctggtc ttggtttatc aattgctcaa aatataattg aattagaaaa tgcacacatg
     7561 gaaataatta tagatgctga tttatttaag gtaatgctta cttttagaag agttaattga
     7621 tatattaata taaaaatata ttttaagtta tttaaacaag aaaaaaacct ccttagaggt
     7681 ctttttctaa atgttggggt ttatatttac atattttttg attatacctt tttggtgtga
     7741 tattaataca ttttaaatta tatttctcta taattcatca catatttcat ctctctgtat
     7801 ctataatatc acatagtgtt tccataatca gttacatctc tgttacctaa gattacaatt
     7861 ttgtaatatt tgtaatctta ggtacaaatt tacatttgtt ctttaatata aatatattct
     7921 ttctatttac cctaaagacc ttttaaatca tatttttcta agaaaagttt taacttagaa
     7981 taaatttgtt ctgctccacc aattttattt gattcaatat ttaaagctag taatggttca
     8041 tgtagtgata ttctaagtaa aaaccatcca tctccatttg attcatcaca attaactctt
     8101 attccttcaa aattatttgg tgctacagac catccatcaa tactcttaac atgagaatct
     8161 aaatctttta atatatcctc tgcatatggt ctaaaatctt ctttgttaat gcctattcta
     8221 acttctttct cttcttgagc ttcttcaagg ttttctatca attcttctat agtctttcct
     8281 tccaaggata gctttgctac ttttattaaa attttagcta ttaaatatgc cccatcatct
     8341 aagaaataat tttcttttaa agcagcatgt ccagacgttt ctatagctaa atggcactct
     8401 tcaccttcac tattcaatct tatagcttca tttattacat ttttatatcc tcttttaaat
     8461 ctatgatgtc ttccttgtaa ttcatttata aatttagcaa gtccttcaga agtaatcgaa
     8521 tctgttacta tagctgtttt tggatgttct tctaatacta ttgatgatat aacagctata
     8581 agagcatttt tatttattgg tttgccattt ttacctacaa tagcagctct gtctacatct
     8641 gtatcaaaga ttattcctag gttgctatta ttatccaaca cagctttaca tatgctttcc
     8701 attgcttctt tgttttcggg attaggtata tgatttggga acataccatc tgggttcaaa
     8761 aactgactgc ctgtcgtatc tgctcccagt atgtgtaaaa ctttttcagc aaagaatcca
     8821 ccagctccat ttcctgcatc aaccaatatc ttaagcccag aaaaaggctt ttcataattt
     8881 ttacttgaat ttacaccttt tctaatttta tctatcaata aattagaata atcttctatt
     8941 aaatttttta ctataacttc acctttttta tttacttcct catagtctac actatcattt
     9001 ttaacagcta tatccaacat ctcttttata ttagtttttt ctaaacctcc actttctgtg
     9061 aaaaacttta aaccattata gtaataaggt aagtgacttg ctgtaatcat aatcgcccca
     9121 tcagaattgt atccatccat aatagttgtc ataaacatag ctggagttgt agcaatctca
     9181 caatcaatta cattacaatc atcattagtt aaagtttcaa taacagtact tctaaattgg
     9241 cttcctgtta tcctagaatc agttcctatg gcaacagtta ctttatcttt cttagtcttt
     9301 tcttttagcc aaatatgaaa cgctttagct atttttttta cttcttctac agttaggtta
     9361 acttctttag aatcattttc atacgctatt cctctaatat cagtaccatt ttgtagttta
     9421 tataggtctc ccataaaaaa ttctccttac tcaaccaaaa tttttattaa atattataaa
     9481 atttcttgtt tccacctaaa tttttactat taaacagaaa catattataa ttttattaca
     9541 tggttacttt atactttctt ttagttcttc tattttatct ttatatgaaa ttggaggctt
     9601 atcattaaat aaagctgaag taccaacaac atatatatct acttttttac cattcataag
     9661 tttaatagta tccttattta tatttccatc aacctctata aatggaggat tatcataatc
     9721 ttttagtatc tctgtaagtt tatccaactt atccaataca gaaagattaa acttttgacc
     9781 tgcaaaccct ggatttactg tcattactaa gataccctca actaaactta tatattcttc
     9841 tattttatcc acacttgttt gagggcttat tgctaacact ggccctatac cataatttct
     9901 taacttttgt atatcagctt ttacatttgt acttgattct acatgaaaag aaatatattt
     9961 tggctttata taagacatca tatcaatata tttatctgga gtttctgtgg ctaagtgtat
    10021 gtctaaaggt attgttgtat tttcacttat tgattttaat aactctggtc ccatagccaa
    10081 gtttttaaca aatattccat ccataacatc acaatgaagt aattttacac ctgcttcttc
    10141 aagagcttct aattcctctt ttaacttcat ttggtctgca cacattattg aagcacaaat
    10201 aatagacata acgctccccc ttgttataaa ttatatatta tatatattta taaattttct
    10261 ttttctatat catcaatcat ttgtaatctt cttgcatgtc tttctccatt ataacttgaa
    10321 tttaaccatt cttcaacaat tctcattgct aatcctttcc caataactct ttctcctaaa
    10381 caaagtacat ttgtattatt gtgttctcta gttgccttag ctgaaaaaca gtcagaaaca
    10441 acagctgctc tgattccttt aaacttattt gctgcaatag acatgcctat tcctgttcca
    10501 caacaaagta tgcctaaatc agatttttta ctttgaacat tttgacttac cttttttgca
    10561 tatattggaa aatctacact ttcttttgaa tttgtaccca catcttcaac ttctatacct
    10621 ttatttatta aattttcctt tatatattcc tttaattcat aaccaccatg gtcacatcca
    10681 atagatattt tcattaaata gcacctcttt ttataatttt tttgattact ttcagactat
    10741 tttataatta tcataaaaat aaagtattgt aaaaataaag ctgtcccatt gtagaaattt
    10801 ctttctattt ttaagacagc tttaattgac ttatgtcaaa tcaatttgtt atttagatat
    10861 atttatatct ataacaactc ttttatattt tttactatat cttctgtagt taaaccatag
    10921 tgttttaata aatcagctgg tgtacctgat tgaccaaata catctttaac accaattctt
    10981 ctaactacta catctttagt ttctgctaaa gcttcacata cagctgaacc taaaccacct
    11041 attatactgt gctcctctgc tgtaactact tttccagttt tcttagcagt ttcttttatt
    11101 aatgcttcat ctaatggttt tattgtagat atattaacaa cttctacatt tataccttct
    11161 ttagcaagtt cttctgctgc ttctaaggct tttgctacca ttattcctgt agctattact
    11221 gttatgtcag aaccttctct taaaacagtt ccttttccta tttcaaattt ataattttca
    11281 ctgtttacat caggagttgc agctctacct aatcttatat aaacaggtcc attataatca
    11341 atagatgcaa gaatagcttg tcttgcttct aaagcatctg ctggatttaa tacaaccata
    11401 tttggtatac ctctcattag agatatatcc tctatagctt gatggctacc accatcttca
    11461 cctactgtta cacctgcatg agtagctgct atttttacat ttaaatttgg atatgctaca
    11521 gagtttctta tttgctcata gcctcttcct gctgcaaata ttgcaaaagt actcgcaaaa
    11581 ggtatttttc cacaagtagc aagacctgct gctgtaccaa tcatgtcacc ttctgctatc
    11641 cccatgtcaa aaaatctatc tggggcaact tctttaaatg atatactttt tgtagctttt
    11701 cctaaatctg catctaatac tactatatct ttattttctg caagttcttt taacacttgt
    11761 ccgtaagctt ctctagttgc tattcccatt cctatagacc tccttctaaa tctttcaatg
    11821 cttgctctgc ttgttctgca tttggagctg ttccatgcca tgcagcatta ttttccataa
    11881 atgaaacacc tttacctttt actgttttag ctattattac agttggtttt cctgtaactt
    11941 ttttagcttc atcaactgca tttcctattt cttcaaaaga atgtccattt attactatta
    12001 cattccatcc aaaagatttg aatttgtcat ctactggatt tacattcatt acttcttcat
    12061 tagaaccatc tatttgtaat ccatttaagt caacaaatgc aattaaatta tctaatttgt
    12121 aatgtgatgc tgtcattgca gcttcccata tttgaccttc ttggatttct ccatcaccta
    12181 atatagtata tactgtatag tctttgttat ctaattttcc tgcaagtgcc ataccatttg
    12241 ctgcagatag cccttgacca agagaaccag tagacatatc gattcctgct acttttttca
    12301 tgtctgggtg accttgtaac ttagaaccaa attttcttag tgttattaat tcttcttctg
    12361 ggaaaaatcc tttttctgct aatgtagcat ataaagctgg tgctgcatga ccttttgata
    12421 gaacaaatct atctctatca gccatttttg gattttctgc acatacgttc atcttatcaa
    12481 aatataatac tgtcaacata tcagctattg ataatgaacc acctggatgt ccgcttttag
    12541 cttctgcaac catttttatt atgttttttc taatattatt agaatgatct ctaagagtgc
    12601 tcatactttc ttcctcctgt tattttttaa gaccttacat ttttttatcc tacagtagat
    12661 tttacaattt tttttaaaat aatacaaatg tttttttaag attttactgc tacttttatt
    12721 tcttccccgt tcattaaagc tgtaatacct tctttaaatt cgtctaaacc aacagttcta
    12781 gtaactaaag cttctgtatt tactttccca cttgccatta aatctaatgc tgtaatccaa
    12841 gaacttggct tttgagatct acttcctata tattgtaact ctctttggat tattgcttct
    12901 tggtccattt catttaattt tttagcaaag attccaactt gtacgaaagt accttttttt
    12961 ctaagcagtc ttaaggcttc gttaactgct ggcataaacc cagaacaatc aaatcctttg
    13021 tttactccat ttccatcagt catttcattc actacatctg ccaaactttc ttccattaca
    13081 tttacagttc tatctactcc aagattttta gctatttcta atctttcctt atcttttgta
    13141 acaccagaca ttataactgt agcaccttga gatttaacaa cttgagctaa taataaacca
    13201 attggccctg gtcctattat taaaactgta tcagaacttt ctatagtagt cttttcaaga
    13261 gctgcatgaa cacaacaagc tagtggctct gtaagggctg ctgataataa agatacattt
    13321 tcaggaagta catgtatgct ttcttctctt gatagtacat actcagcaaa acttccattt
    13381 acttgagtac caataccttt tctagttggg cataagttat aatctttagt ttcacaatat
    13441 atacatttct cacaagttgc aaaagtagtt tctgatgtaa ctctatctcc tacttttata
    13501 tctttaacat cttctcctac ttctacaaca attccagaaa attcatgacc taatgttact
    13561 ggggctttta tatttgcata ttcaccttta aatgaatgta tatctgaacc acatatacca
    13621 ctatattcta cttttatttt aaccttgtct cctgtagctt ttggttcttc tacttctaaa
    13681 acttctacat tatcatagcc tggtttagtt ttaactactg ctttcattat ttttcaaccc
    13741 cttgtggtaa aattaatatt ttgttaaatt cttcttttct atctcttatc atttcaaatg
    13801 ctttgtaagt atcttcaagt gagaatctat gagataccat ttcttttaat tttatctttc
    13861 cttcattaac tagattgatt cccttagtcc attcttgtcc tggaaatggt gcagaataag
    13921 agttccaaaa accttttaat tctagttctt ttctaaatat attttcaaat gcttcttctg
    13981 ataaagttat atctgaatat gctatcccta aaaatcctat cttacttttc tttttagtta
    14041 taagtaaaca ttgttcttga gttatttttg aacctgcaca ttcaagagct atgtcaacgc
    14101 cttttccacc agtaagttct tttatttttt ctactgggtc aatatcttta gcattgatta
    14161 ttatatcagc ccctaattcc tttgattctc ttaatttatt atcagatata tcaactgcta
    14221 taattgttcc tgctcctgct attttaagtc cttgtattac caactgcccc attgttccac
    14281 tacccattac ggctacagcg tctccaactt gaacacctat attaagtact ccatgcatag
    14341 caactgctag tggctctatc attgcagcat cttcaaagtc catatctcct attggaagta
    14401 catttgtagc ttttatattt acatactctg caaaagctcc aaaactttta gctcctaaca
    14461 ttccatggtc atcacacaat gaaaataatc ctcttttaca atattcacat tcattacaag
    14521 gtataaaagg aattgcaact actctatctc caacttttac attttttaca tccttaccaa
    14581 cttcagcaat tactccagaa aactcatgtc ctggtatagc tggaactgga tatttccaat
    14641 gttttaatac tcttggagga tctgacccac atattcctgc tgccataact tttattttta
    14701 cttcatcact ttcacatggt tttatttcta tatcttcaaa tctcatatca tttgtgccat
    14761 gtaatactgc tgctttcacg ataaatctcc tcctcttgta ttttgtattt ttaaaaattt
    14821 ataaaaaata ttactctttt ttagtagctg ctaaatgtta aattttcgca tactttacta
    14881 aaaaagagta atctctatta actacatatt agaatattcc aaatgaagaa actaattgag
    14941 ataatttcaa tattccccat tttagcaagt ttccacctgt atctatactt gtaaccattg
    15001 ttgttccttc tggcatcata tttgcttgtt gtaataattg tgtatgaaca tctgcaaagt
    15061 ttgtagccat ataaagtgat atagctatta ctattgttcc tgttataact gattgaacta
    15121 tattaccttt tctaaagcac actataaatg ccatataaaa tggtattgtc gctaagtctc
    15181 caaatggtaa aactttgttt cctggtagta taactgctaa gaacaacgtt ataggaacaa
    15241 gtataagagc tgttgatata actgctggat gtcccgttgc tactgctgca tccagaccta
    15301 tataaagttc tctgtctcca tattttgctt gtaagaaatc tcttattgat tctgaaattg
    15361 gaattaaacc ttccattaat attctaacca ttctaggcat aagtaacatt actgcaccca
    15421 ttgatatacc tacattgaat attcctgcta catcatatcc cgcaagtgct ccaagtatta
    15481 tacctagtat aatccccatc atcattggtt ctccaaatat accaaatctt ttttgtatag
    15541 agtctgggtc tgcttgtatt ttatttattc ctggtatttt ttcaacaaat ttacctacta
    15601 aatatccaag tggagcaaat gaagctgttg aaccagttgg taatgctata ccttctaaat
    15661 cataaaactc ctgaacattt tttgctgtct tttctgcaat aaacagtact aataactcca
    15721 ttagtattgc acagaatata gcccaaacat aacttcctgt tactatatat ccaactgcac
    15781 ctgctactat aaagtgccaa taattccata tatcaacgtt taatatcttt gttttattga
    15841 agtataataa tagtaagttt actaataaac aaactggtat taatattggt gcaactggtg
    15901 aagcccatgt tgctgatgct gttgttggcc aaccaacatc tattactgtt aaattaagtc
    15961 cgaatctttc aaccattcct tgagccgctg gccctagtgt tgatgttaat aggtttacaa
    16021 ctaagtttat acccacaaat cctataccaa ttgtaagacc tgactttaaa gcttttgtaa
    16081 agtacagttt acaagggact acacagggta ggtatctgac gggaggggtt ccgcccgtca
    16141 gattttccat gtgatgttca agctgtcgct tgtggcggct atggtggtaa tcatcaaatc
    16201 caccacacgc cgcttgtcat caaaggatac atcctcccaa gtgtcgaggt agccggaaat
    16261 ctggctgacc tgttccgggc tgatggcttc caccgtcaac tccgctatcc tcgccagaag
    16321 ttcctgcttg cgtccgtcca gttccgctat cttcacattc acataggaga gcagcacatt
    16381 gtttgcgccc gtcagactgt ccaccagttt ttcaatctcg ccgtccacat gggcaagctc
    16441 cacttgcagg gcggcgattt tcgggtttgc ctttgccgct ttttttctgc ccgtcagcgt
    16501 tttgtagctt gccagtttct ttatcatctg ctgataaaca accgtttcca gttccgaagt
    16561 gatgattttc ccgcaccccg gacaactctt gttatccagc cgtttcgtac agcgaaggta
    16621 ttgcttgccg gagggattga agatactcat aagggcatac ccgcagttcc cgcacttgat
    16681 ttttcccgcc agccatgtgt gggtggcttt tcgggcagac tggattttca tgttgttcat
    16741 cagcttcttg cggcaggtca gccagatgtc ggaggggaca atgccctcat ggggagccag
    16801 caccagcatt tggtctttca agtcgttctt tttgctgggc ttcacatccc gcccttgata
    16861 cagatagcag ccattcatgc cggtaaaatc ggcagcgtca ttgacaatga ttgtcccttg
    16921 acttttgaaa aattcgtaca catcaaggtt tgcctgcaca tagacaggat tgcgtaacat
    16981 ctgcgccagc gtggggcgta ttagttcctt gccgttgaat aaaattccct gttcggcaaa
    17041 gtaccgggta atgtccccgt aggaggttgt gggctgggcg tacatctcga acatcagccg
    17101 gatatttgcc gcttcgtctg ggtttaccac cagcttcttt gtgttgatac cgtccatctt
    17161 gattggctct gtatggaagc cataaggggc ttttccaccc atcttaaacc ctcgctggct
    17221 gcgggaataa taagcgtccg tcacccgctt ctgtatcgtt tcccgttcaa gctgggcgaa
    17281 cacgatacag atattcagca tcgcccgccc catcggcgtg gaggtgtcaa acttctccgt
    17341 agaggacaca aactccacat tgtactgctg gaacagctcc atcatattgg caaagtccag
    17401 aatggaacgg ctgatacggt cgagcttgta aaccacgacc tttgcaatta agccccgctt
    17461 gatgtcccgc accagttctt gaaactttgg acggtctgtg ttcttgccgc tgtacccttt
    17521 gtctgtgtat tccttgcagt taccgccttt caactcgtat ttgcaaaatt caatctggct
    17581 ttcaatggaa atgctgtcct ttttgtctac cgattgtctt gcatagattg cgtctattct
    17641 gttgttcata ttgtcgctcc ttttcttcaa aaagaaacgg agctgctgac agttttatta
    17701 taccgccagc agccccgtaa atcaacgatg gcttcggaaa accttacgcc ccggcttcct
    17761 ctttctgccg cttatcggca tatttgcgga acacctcata aagctgctgc tccagctccc
    17821 ggcgttttgc cgcttcctga tccggcgtga acaccgggga gagattttcc agcgtgattt
    17881 cttttccctg aaaagtcacg acctctgttt cttttttgta tctgatatgc tccatagaac
    17941 ctcctgtatt caattttcaa ggtacaatgc cgcacaggga cggcgtaaaa atctgcttat
    18001 aaaatcacct ttcctttcca tgctgccgct gctgccgttt cagctccgcc agtatatccg
    18061 gcgggatacg gtcaaccagc cgctgtaaat tgtccagttc gcttttcagc tttgcccgtt
    18121 ccatcgtatc tttcatcttg cctttttcac tggcttttgc ccttgcttcc agcttttcat
    18181 tttccgctaa caggtcattg attgtgacct tgtatttttt cagttgcccg gagaaattct
    18241 ccatctgcgg gaaccacttt ttcagcatgg agagggcttc ctcttttttc tttccggcgt
    18301 tgaacggggt aatatcgccc agcgtggctt caatggctct tgcctgtttg gagagattga
    18361 ccgcctgctt gaacagacgg gtggggatat gcttcctgcc tgtcttgctg gcactttccc
    18421 cacgctccaa gtcgggatat ttctccacca tataggcatg aaaatcgtcc tgccactttg
    18481 tcaggtttgc ccggttgccg ataatctcct ttgcacacag gcggttgtcc tctgtcagcg
    18541 ggacaaagac caaatgcagg tggggtgttt tctcgtccat gtgtaccacc gccgacacga
    18601 tattttcccg ccctacccgg tcaatgagga agtccgccgc cctctggaaa aattcctgtt
    18661 cctcctttgg ggacttaccc ttgaaaaact ccgggcttgc ggtaatcagc gtatcgacaa
    18721 agcgtgtgct atccttacgg gtgcggcagc cagcctgttc aatgcggttt tggataaagt
    18781 ggtaatagcg tccctctggc ttgacaatgt ggaagttgta cttgctccgg ctggtgtcaa
    18841 tatccgggtt gctggcatac tgctctttct gcctttcgtg atgggcttcc agcggtcttg
    18901 ccggattgcc cttgtgtttt tcaaatcgca aaattgcgtg ttgtgccata aactcctttc
    18961 cccgttcctt tcccttccgg ggcttttcca caggaaaatc ccgcagaaaa tatctcggat
    19021 aggaagggaa tggatagaat ataaatcaat atttttatct ctgtatttct atataccgtc
    19081 cggttttcgt acttcaagag gattgattat cgtacttgtg gagtgcggtt ttcagtcctc
    19141 cggcgtacca gaacgggatt tcttgaagtc ggtatttgga accgcttcat aggattttgg
    19201 gtagatgcgg ttgggttttc cacagccctg cttctggatc tccaccagtc cggcatattg
    19261 cagttcccgc agggtattga ctgccttttg ccgcccgcag tggagcagct ccaccacctc
    19321 gtagattggg taatacaggt aaatccgccc gtactcgtcc gcccagccgt tcttgcggga
    19381 taactccgtc cggcgcagga taaaggcgta cagtagcttt gcttcattgg gcaaagctgt
    19441 gaatgtgggt gcttcaaaga ggaaattcgg gagccgggtg aagctgaccg atttctccgg
    19501 ctgatggata taaatggtat ttatcatagt gcgttttgtg gacggttaga agcctgtttc
    19561 cgggggcaga cggtagtttc tattgcctgc ccctgttctg tgcttgcaaa gccttgtaaa
    19621 tcaatgactt ttcagcccct aactgtccac agtagacctc cttttccgtt cactttctgt
    19681 tttctgccgc ctgtgaactc tggcggcaca gccgggacag tatttcgccc ggttggactt
    19741 tgggacgaac actctgccgc agaccgcaca gcgttttagc tccttatccc ggaaaatctc
    19801 cgcttccagc gttccgactt gcggcaaaac cgaccagcgg aaccacttac agcagaccga
    19861 gaaagaaatg gtctgggggc aggtgtgggt gtccccatcg tcaaggggca tacagttccc
    19921 gtcctcatag ttgcagcact cccggcggat taaggcgttt gcctgtttcc tctgtgccgg
    19981 tgtcatacgg taaagggaac cgtccggctt gcgctctatg ggcggcagtc gtttatatgg
    20041 gttctctctc atgtgtttcc tccattttgc tttccgttgc cgctctctcc gaaaaggctt
    20101 cctgccccat tcctgcggcg tgttccggcg ttggcacgct ccccatagct tcgggacaag
    20161 ttgtcccgaa gtgacctctg gacaaagtgt ccagaagttg gctccttgcg gtgcttcccc
    20221 tgcctgggta ttccttttcg gacggtcatt catttttcaa ggtgcagctc atcgatgaac
    20281 taccctaagt ctacacctaa aagacagccc gtcccgcata tccgaaaggt aggaaatccg
    20341 ccaaaaaaac tgcctatttc cacgctctcg gaattgtggt agaatgaaaa agcagaacaa
    20401 taaacgataa ttttggagta cataccgata tggaaattat agaaatcaaa gagaataaaa
    20461 aacaatttct ccccttatta ctgttagccg acgaacaaga gagcatgata gaccgatata
    20521 ttgatagagg aatgatgtat gtattagtcg ataatggggt taaatgcgag tgtgtagtaa
    20581 ccgatgaggg caaaggcatt cttgaaatta aaaatattgc aactgcgcca gaatatcagg
    20641 gcaaaggtta cggcaaggca ctaattgact ttgttgctgc gaaatataaa gggaagtatt
    20701 cgatactaca agttggtaca ggggatagcc cgttgaccgt tcctttttat gaaaaatctg
    20761 gttttgtccg ctcacacagc attaaaaatt tttttattga taactatgac caccctattt
    20821 ttgaaggtgg ggttcaacta acagatatga tttatttaca gaaaaacatt ttatgaaatc
    20881 caacgcaaag cattgacttt accaccgttt tggtgtatat taaaatataa tattatttta
    20941 gatttgaggt ggaaaggtga ttaaattttc ttgctaacta tttttgcatg gcatgaatct
    21001 tcgggcatac acgctatgcc gatgtttttg ctgtgcgttt gcaagaaagt ttaatgccga
    21061 ttcactcata tgagccttgt tttgccgcct ttatgcggct ttgacttcgc tatcaatatg
    21121 ggatgcagga agtaactttc ttgcatccca tatttttatt gcggaggttt gactgatgtc
    21181 acttatcaat atttcgaatt taaccttttc ctatgaagga agctttcaca atatttttga
    21241 aaacgtaagc tttcagattg atacagattg gaagcttgga tttaccgggc gaaacggccg
    21301 gggaaaaacg acttttctaa agcttctcat ggggaaatat gaatacagcg gtaccatttc
    21361 aagcaatgtt tcgtttgagt attttcccta tgacgtgccg gacgctgact tttttgtcat
    21421 tgatgtcatt catgaaatca gcccgaatgc gcaggactgg gaaattgcac gtgagctgtc
    21481 cctgctgaat gtgtcggatg atcttcttta tcgctcctat tccacccttt caaaggggga
    21541 gcagacaaaa gcactgcttg ccgctttgtt cctgaaagaa aacagctttc tgctgattga
    21601 tgagccgacg aatcatcttg acactttggg cagaaaaacc ttgagcgatt atctgaagcg
    21661 aaaacatggg tttattctgg tttctcatga ccgggttttc ctggacaact gcatcgacca
    21721 tattctcgta atcaataaaa caaacatcga ggttcagagg ggaaacttct cgtcctggtg
    21781 gaggaacaaa gagatgcagg acagctttga acgggcagaa aacgagaaac acaaaaaaga
    21841 aatcagccgt ttaacagctg ccgcacaaaa gacttccggt tggtcagacc gtgtggaaaa
    21901 aagcaaaaac ggttcgacca actcgggaag caagctggac aaaggctatg tcggccataa
    21961 agcagcgaaa atgatgaagc gttccaaagc tattgaaaat cgtcagcagg atttgattca
    22021 tgagaaatcc aaactgctca aaaacctgga aactgccgaa agcctaaaac tttctccgct
    22081 tccataccac accagccgac tgcttgagct ttccgatgtt tccattgcct atggaagtcg
    22141 tgccgtctgc tcgaatatca gttttaccat agagcaggga gaccggattg cgctgcaggg
    22201 gaaaaacggt tccggaaaat ctagcattct gaagctgatc tgcggggaaa acatccccta
    22261 tcaaggcatt ttgcgaaaaa gcgaacggct gaaaatttcc tatgtccccc aaagcacggc
    22321 ggggctgaac ggcttcttaa gcgaatatat cgagcgaaat cttctcgatg aaagtctttt
    22381 caaagccatc ctgcggaaat ttgattttcc tcgggagcag tttgaaaagc ggttggacga
    22441 attcagcgag gggcagaaaa agaaagtcct gctcgccaaa agcctgtgtg agcaggcgca
    22501 tctgtatatc tgggatgaac cgctcaacta cattgacgtc ctttcccgca tgcagataga
    22561 aaacctgctg ctcgaataca agccgaccat tctgttcgtg gagcacgaca gtgctttctg
    22621 tgaaaatatt gcgaccaaaa ccgttgagtt atagaaagga gaaaagccta catgatttat
    22681 tcaaaatatg aggttatgaa gcgatgtata tcgggaatga attttccgga ttatcgctat
    22741 gagcaattga taaagatgat ttttgcacag catattcctg actttcattc catgtatatg
    22801 ctgccggaaa gactaagatc aaatttggcg gaaacttttg ggacctctgt ctgcggcctt
    22861 gttccagtta cacatcgggc atcgggccag gcagataagg tgctttttca attaaaggat
    22921 ggaaactgcg tcgaaacggt gaacctgcat tacaaaaaag gatgggaatc tttttgcgtc
    22981 tcatctcaat gcggctgtgg gtttggctgc aaattctgcg caacaggtgc tatcggacac
    23041 aaacgcaata tgaccgctga tgaaataaca gatcaaattc tgtattttta tcttgcaggg
    23101 catcaaatca atagtgtttc ttttatggga atgggcgagc cgtttgcgaa tcccaattta
    23161 tttgacgcat taaaaatcct gacaaacccg tctttgttcg gattaagcca gcgacgcatt
    23221 accatatcga cgattggaat gattcccggg ataaaacggc tgacccgtga ttttccgcag
    23281 gtgaatttag ctttttcgct gcactcgcct tttgaaaagc aacgttcgga attgatgccg
    23341 atcaatcaaa cttatccgct ccataaagta atggatgcgt tggatgcgca cgttgcaaat
    23401 accaaaaggc gcttgttcct ggcttatatt atgctgggcg gaataaatga ttcggcagag
    23461 catgcgaaag cgttagcgca ccttattttg agccggggag cgttgtccta tctatatcat
    23521 gttgatctta tcccatataa cgcaacagat aagacggcgc gaaaatttac cgcttcaaat
    23581 cacgaagcca ttaaaaactt cagtgatatt ttacattcca accgtatcag cgtggctatc
    23641 agaatgcagt tcggttctga tatcggcgcc gggtgcgggc agttgtatgc tgatgaaagg
    23701 gattgagcgc ttcaagacgt ttttatgtac acagagattt ccaagcagca ttggcgtttg
    23761 aaaggaaagg cacccggcat aaatttaatg ctgggtgcct tattgttaca tcaaaaatat
    23821 aaattcccat ttatcgagca gacaaggagg tgatagcatg gaactatcca tacaggaacg
    23881 attgaaagac ctgcgtgtgg aacgagggct gacgctggaa cagcttgcgg aggaaactca
    23941 tctttccaaa tctgctttag gcagttatga gggagacaat ctcaaagaca tcagccacca
    24001 tgcccttatc cagctggcaa aggtttatga agtgaccgtt gattacctgc tgggacgctc
    24061 taaaacaaaa aatcacccaa acgccgatct tgcagacctg cgcttgagtg atgatatgat
    24121 tgaactattg aaaagtgggc gggtggatac ttccctcttg tgtgagctgg cggtacaccc
    24181 ggatttcccc cggctgatgg ctgaccttga aatctatgtg aacggaacgg cagtcaagca
    24241 ggtacagagc gcaaatgcca ttgtggatat tatgagcgca gccattatga agcagcacaa
    24301 tcccggcttg tccgacccgc agttaagaca gcttatcgcc gctcatattg acgatgacag
    24361 cttttgccgc tatgtgatac agcaggacat aaacaagata gctcttgacc tgcgggaagc
    24421 ccataaggac gattttttca gcgtcccaga ggacaatccg ctggaagatt ttttacagac
    24481 cgccgaggaa accgctaaag agggcagcga cccggagcaa gcgtcgctgg cgtttatctg
    24541 caagcggctt aaactgaatt tgaagaagct gtccgaggaa gaaaagaagt ggctgaaaaa
    24601 gattgcgcag aaatcggact tgctgaaaaa tccaaaccca cagcggggga gaaaatagag
    24661 aacgagaaat tggagggagt aaaggtgcag gaggcaatta taaaattgaa acttctcggt
    24721 caaatgccag acgcagtgaa agatgaccct acagaagaaa ccatcaacat gtatgacgaa
    24781 ttgctctcca atgtaaaaac gccattgaca agagaagaag taggcgtatt gattgacatt
    24841 ttccccgaag gtggaatgta tggagtagag tgggatttgc taaaattggt agagtcttat
    24901 ctgatagaag caccatcaag tgaagagtat cgaaaattga ttactgcttg tccaagtgaa
    24961 gaatggcgag aaactatgca agcccgttta gataattggg aaaacaacaa acaataactt
    25021 gttggggaga tagcaaagcc agtcgagcca gtcaacggtc aagatgaacg gcgcatttca
    25081 tgcgccgccg ttgacagccc cgcccgtctt tgctaatggg taatcaaggc gggaaagccc
    25141 taaaatggct tcccgcccat ttcaattttg agagaaaaat ccgtctgctt tttcgtaagt
    25201 gtggctatcc gtctgggaca ctttgtccca tagttcggcg taggacgagg gacgggggca
    25261 ttcatatacg ccctgtctgc tgatgaaacc agtcctgcgc ttgcggctcc acgccacgca
    25321 atcacagccc tggtttcctg ccgtaaaaat gcccttgccc tccccggcgg caaccgcatt
    25381 ttcgcagcaa cgccgacaga gcgtataaca cactacactt tgcgagcaaa gtcgtgtgcc
    25441 aaggggcaaa cccctttgga aacccctgac aacaaaacag gctgaatatc tcgcactttc
    25501 ccggtgcttg atattcagcc tttatttgtt gtcagcagcc cccgtttcgt ggtctgcgtg
    25561 tagttccttg taaactgacc taaacttaat tctaaatatc attcctagta ctacaattgt
    25621 tattggtagt attactgtcg gtcccatatt taaaatgtat tgtactactg ctaataattt
    25681 atccatttat aaatcctcct ttttctgttt tataaaatat tatttcaatg tatctatgat
    25741 aggattattt taaagcatct aatatttttt tatctaatgc tgctgtattt actccagtta
    25801 tatatgctgt tgcttttatt gctggtatat tataagttgt tggtagtata gttgttgata
    25861 ctattaaatc tgctccttct tgctttgttg cacattcagc tattttgcat tgtataactt
    25921 ctgcttgtat attattttcc ttaactaatc tctctacctt gtcacaaact actgttgatg
    25981 ttgctattcc tgctccacat gctactaata tttttttcat ttttatttcc tcctaataat
    26041 ttttattttt atatttttta tttttagtac ttttttattt ttataaagac tttagtgtcc
    26101 ttaaaacttc ttctttatca gtagaactca tcaacttatc aactaactct ttattttgca
    26161 taattcccat taattctgtt aatacagata attgactgtg tggttgatta agtcctaata
    26221 tgaaagccaa atttacaggt actttttcat cttggtcttc catagaacta aatactactg
    26281 gttcattaaa agtacagact gatataaatt gctctttaat atattctgca tctgtatgtg
    26341 gtattgctac tccataatca cctaaattta aacctgttgg aaatacattt tctcttgcta
    26401 atattttttc tccaaattct tcttttacaa agcctaaatc aaaaacttta ttatgtattt
    26461 ctttaaagaa atcttctttc gttttacaat ccattttaag gtctataact ttatcactta
    26521 caaaatttat aaactccata tttacacctc ctaattgcct ttgtcttata ccttagtttt
    26581 tcttgacttt atataaagca tgttgcgtgc caaaaagtgg ctttttttgt gaaacaaaag
    26641 aaaatgtttt cccgaaaata atgctctctt ccaggatttt agctgacttt cattgataaa
    26701 ttttttttga tttttatttt aatttttact tgttaatcac ttaactagcc atactttttt
    26761 atattttttg tggttttttt tgttagagaa atgatttttt tgttttttac aaatggtttt
    26821 ttatgtttaa caactatttt ataaaattat ttattatgaa tttaatttat ttttatatat
    26881 tttttatata ttctatattt tataagaaat ataggaaaaa ttttcataga attgtccaat
    26941 aaaatttact ttttacataa ataaattcat atattttatt taccaagaaa aaagctaatt
    27001 ctataaaatc atataaataa atttcataga actagcctta tatagagtaa ttaactactt
    27061 agcaattgag taattttaaa ttttatacta aagcaaattt ttttgtcttt cttctcggta
    27121 ctaataatta gcttaaaagt tcttttattt catttttatc ttcagtattt aataaaactt
    27181 ctaaagtatg ctggtcatta atctttgaaa ataagcttcg tatttctttc ttattatttt
    27241 ctttaagtgc cagtagtacc accaaatcca ccataaattt attgtcccat actataggtt
    27301 tattcaacct tgcgattgct attgcaggct ttataacgtt ttctggaagt ccatgtggca
    27361 cggctacctt agaggccatt acaacacttc ccattgcttc tcttttatat acacttaata
    27421 tatactttga atcaacatat ccaccttctt caagttttga aacaagagta tcaattatat
    27481 actcctttga aattccttca aaattgtata aaatcaaatc ttcatgtatt aaattattca
    27541 actggttttc aatatttaaa gctggttgtt tactatttag atgctctaat actttattaa
    27601 taccattttt accaagtacc tcattataag aaataaaagg cacgttttta taatctatat
    27661 caatagttcc acagatagca ataatattat attcagcttc aatatcattt aactcttctt
    27721 ctaaattatt atttaaaaaa gcaactggct ttacttcaat taaagtgcca tattccttaa
    27781 tttgtttttc tatatgctct tttaaattta aagctgtacc tacaccactc aaacataaag
    27841 taatgattac atttttcttt ccatactcgt tttttctgta actaaattta tttgtattta
    27901 tcatagcata attcttattt ttctttaaat cagctattat accatctaaa ctttttcctt
    27961 caatggttgc aagttttcct gcttccattg ccaataatgt atctactcta tttacagttt
    28021 tacatctaat tccaagcctt ttttcaacct catcaccaag aattaccaaa gaacccatat
    28081 caattagaac tagaatacct tttctagctt ctatcttttg aagctcttct aatacatgct
    28141 ctatcccctc tgatggtgaa tctgtaagtg ctatttctat acctagtgta cattctatac
    28201 ctaaaatatg atttacaact ttagacattt caattccaac tttaccatgc gtaactatta
    28261 ctactttaac tttatctttt atactttcct cattataaca aaatttattt atataataag
    28321 ccaaatatcc aagctcatct tctggaaatg ttacttgtgt caaatcttct actataccca
    28381 ttatatatct tgacaactca tattccattg gcattgatgc ttttatttta tctaaactcg
    28441 gatttattat agtttttcca tcttttatcc tacctatagc atgctcaata tgaattgcta
    28501 gagccaagta cagaccttga tttacatgct taaactgctc cttaataaat acaattacct
    28561 tatccattat ctccagtgta gactctttta attgtcctcc ccagtctatt cgacttcttc
    28621 tatcagataa atttttattg tttttaatag cattaaactt cttatctaac ttttctataa
    28681 atgcattttt taactcttct tctgaaaagc acttactttc caaaacctta agctcttttt
    28741 ctgcataatt atatatctcg cttatctcat tatcagataa aattctattt tttccactca
    28801 ccaattcact aatatctata tatatatcct caactgaaaa ctcagaatac ttttcatttt
    28861 tacaattaca atcataatat ccacttttta tataatccct taaactatct aaatctataa
    28921 ttaccttatc gctagaaaat atcgatttac taaaagctct agcacaagtt acttgtatat
    28981 cactctttaa ctgacctata tttccagtac acttataatt cataagtgca cacataactt
    29041 ctttagttat tataaaattc ttgtttactc gctttgcttc tctttcaaaa aactttatta
    29101 ttatttcata tctttcagat tttggtcttt cactcatatt aggtaaattt attaccattg
    29161 gtatacgtct tctaaatgtt tgaagtaaat tagaatctgg attttctgtt gtagcactta
    29221 ttatgagaac atttgccttt ctattagaac tactttctcc taaccttcta aattctcctc
    29281 tatctattaa tgaaaataag atttcttgcc cagcagcagg caatctatga atttcatcta
    29341 aaaacaatat tccattatta gcttgttcta caagaccttc tctatttgca tctgctcctg
    29401 tatatgcacc ttttactacc ccaaagagtt gagctatcaa tagattagga ttttctgcat
    29461 agtctgcaca gttaaacact ataaatgggc tgttctcttc tttaatgttg ttttttatag
    29521 catatttata catacatgta gctaattcac ttttacctac tcctgtttct ccatatatta
    29581 tagtatgaag gccattagga ggatacatta ttgctgactt agctagattt accttttctt
    29641 tcaaacttcc ctttatacct atcaattctt caaatccatc ttcttctaca actttactca
    29701 ccttcacata ctttcctgat gatgctgggt ctttatcctt aatcccaatc tttgactgat
    29761 ttaaattttc ctcttttaat aattttgaaa ctgcacttct cgatatatta tatccatttt
    29821 ctattaatat ttcagttatt tttctttcag acatttgagg attttcactt acaatttttg
    29881 atatatcttt tattaatatt ttttttcttc tttctctcga atctaaaata ttattttttt
    29941 tcctaactga tatcacttca gacctagcta catttaacat ttcagcaagt tcttggtcag
    30001 tatatggatt ttttttatct tctctttgta tctctcttaa tattttatcc atttttcgcc
    30061 tcctcactta aaaaattaat ctatttcaat aatatgtttc aatatattat acaccaaaat
    30121 aagactaggc aaaatagcct agccttatta cttagaattt ccataataac ctctttaata
    30181 tacttataaa gcgtagttta taattataat tttaaaaaag caccttccca atccttcata
    30241 aagctatcta ttcctttgtc tgtcaaagga tgcttaatca tttggtttat aactttaaga
    30301 ggaactgttg ctatatctgc tcccattcta gcagcttgta aaacatgtat tggatttcta
    30361 acactagcaa ctattatttg agcctcaata ctatttacac taaatatatc aacgatttct
    30421 tcaattaaat caagaccatt ttgtgatata tcatccaatc taccaacaaa aggacttaca
    30481 tatgtagctc cagctctagc agctaaaaga gcttgtccag cagaaaatat aagcgttaca
    30541 ttagtcttta tacctttact tgatagtact ttcaccgctt ttagaccttc tgctgtcata
    30601 ggtattttta taactatatt tttatgtatc ttagataatt tttctgcttc ctctatcata
    30661 cctttatgtt ctaaacttat tacttctgcg cttattggcc catctactat ttcacttatt
    30721 tctttaacta cctctataaa atctctacct tctttagcta ttagagacgg attagtagtt
    30781 acaccacata ttacacctaa atcatttgct tcttttattt cttcaatatt ggctgtgtct
    30841 ataaaaaacc tcataaaaaa tcctccataa ttacatagtt aaaatatgtg ctaaacacaa
    30901 ttatataata tcataaatta cctaaatttt acagttccat tatctataga ttcaactact
    30961 gccaaagact ctaatcttac atcattagcc tttaaaagtg ctcctccagc ttgaaatcct
    31021 ttttcaatga caattcctac tccaactaag ttagcttctg cttgctcaat taaatcttta
    31081 agtccaagag cagctcttcc atttgccaaa aaatcatcca ctatcaatat attttcgcct
    31141 ttgtttatgt actttttaga aactttaact gaatactctc ttgccttagt aaatgaatat
    31201 acattagttt catatacatc tttatccaaa tttttagatt ctgatttttt tgcaaatact
    31261 acaggtgcat tatcaaaata ctgtgaaaca attgatgcta tagctattcc tgaagcctct
    31321 attgtaaata tcttatccac cttttctcct ttaaatctct ctttaaattc tcttcctatt
    31381 tcatttaaaa aagctacatc aatttgatgg tttaaaaaac tatcaacttt tagtatatca
    31441 tttcctgaaa cactgccttc tctcaatatc ttttcttgta aagccttcat tgaacttcca
    31501 cctagccctt tctaaaatta tattaataaa aaaattatat ttgttctaat tttgctatct
    31561 attcttgtaa gtattttata ttaaaatact aaaaaagctc ctgtctatat catagacagg
    31621 agcttaaaat tcaaagtaat taacttaata tataatttaa aataagtata aactatatct
    31681 tcttcactcc tgtagtcaag ctattaatgg tagcttagta gaaacatttg aaccatatta
    31741 tcaaacatat acaggtaaat atattaattt attcctttta attatatatt ataattctct
    31801 attattcaac aaataattca aactgtgtag aataatatat tatagttact ttatcagaac
    31861 tacttttttt caagtaaatt tataaatttt tcataaatat ataacttttt tcgatattat
    31921 ttaaaatttc ttcgtcattg cttatattag ctattttagc aacggctttt tttaccccaa
    31981 tagactttat caactcttgt aattctacac tttgagtatc ttcactatta ttataatgaa
    32041 gcgctgcacc tattccaaga atcaaattgt caactgataa tccatatgat tttgctgtca
    32101 ttaaaggttt aatcaatcta tctttatcac ttagttttct taatggttcc cttccgactc
    32161 ttaatacatc atcattaaga tatggatttt taaatctatt taaaatttta tcaatatatt
    32221 tatagtgcac ttctgagtca aaattgtatt ttttaatcaa tccttctcca ctttctacca
    32281 ttgcactttt gactatatca cagataaatt tatcttttat actctcttct atagtcttat
    32341 atccttttaa atatcctata taagctgtta tagcatgacc tgtattcagt gtaaacaatt
    32401 tacgttctat ataagccata aggttatcag ctaaattcat tccaactata gttggtattt
    32461 cccctttaaa tccttgtttt tcaacattcc actcataaaa attttcaact gttacatcaa
    32521 gaggattttc gtttttacca ggaggaacta ttctatcaac tgaacagttt ggaaaaccaa
    32581 catacatctc taaatattct acttcttcct tatttaaata cttaagtacc tcttctttta
    32641 aagaagaact tgcgtatatt gcattttcac atgctattat atttagatta cttgttaatc
    32701 ccttttcttt tctagctttt ataccttttg caattgtaga ggctatttta gtcaaaacta
    32761 aaggtccaac tgctgtagtt attatttcag cctgtacaat ctcatctatt atttcttctt
    32821 ttatagatga aaccgcactt atattatcaa ttacttcatc tatacattct acatctctta
    32881 tgtgtatagt atacttttta tctttgttta ttgagtttaa tatctcctca tttacatcag
    32941 caaatactac atgataaccc gacttaacta acaagcttcc aataaaacct cttccaatat
    33001 ttcctgctcc aaactgaatc gcctttttca ttttaaatcc ctcctaagat tagttggcaa
    33061 aagcttccat aaaagcttgt ttgtcatttg aattttttag tctttctgtt aagtcatcat
    33121 ctaaaactac tgctatatta gatagtattt ctaaatgttc atctccaaca ccagctattc
    33181 ctattaataa atatgctttt tctccatcaa aatctatacc attagggaat tgtaatataa
    33241 ctattccaga agataatatt tctttttttg cttcatttac accatgtgga atagcaactc
    33301 ccattcccat atatgtagtc attacttttt ctctttctaa cattgctggt atatattctt
    33361 cttttacata tccattttca actaattttc ttcctgctaa agttatagct tcttcctttg
    33421 aaacactatc taaccctaaa aatatattat tctcattcaa tactttactc atagtatcaa
    33481 ccttcctttt taatattaat tttatttatc tataaatgct tttaatctgt ttgtataaaa
    33541 attaaaaact atatctttta cctctaatgt catctcttct atagagaatt tatttatctt
    33601 attagtaaaa attatattgt ctattaatga accacttatt tcactcatta tttgtctttg
    33661 ataacttggt gaatttttag gaagtaacat aaaaactaca ttatcgattt cttcatttga
    33721 atctagcacg attctattct ctagttttat tattgataac ttcataatat ctattctttc
    33781 actcatacaa tgtaacaata gaatttttga ttcttctata tatggaattg agatttttaa
    33841 tctttctttt aacgattctt ttatagagat tatatcttca ctagattttg caaatatttt
    33901 tgatgagtct tcaataagct cacttaaatc tttactttca aatttttcaa atctaatttc
    33961 ctctaaaaat tgaagaatat cttttcctat attcatactt tctgttatct gttcatatac
    34021 tttattctta cttttagtat catttttcgc atttaaattg attaatttat tttgagctat
    34081 agattttatt ttttccttga ttatctgttc atcatctaaa ctcataaatg gacctacaca
    34141 tatataattt aaacttgtat ttaactcaac tgtagacact attaaatcaa catctttttc
    34201 ttttaaatac tcttcatcta tgtttatagc tgatatggtt tcaagtatat ttaaattagg
    34261 aaatttattt tcaatcttag ttgataagaa tcttgaagta cctattccag tgggacatgc
    34321 cacaacaata tttatattag tattcatcat aagatttttt tctatggctg atgcaaagtg
    34381 cattgcaata tatcctattt ctgattcagg tattgaattt atatttagtt tatcttttat
    34441 aattctagaa atcttttcaa ctccatcata ggcatatgaa tacttgcttt ttatttcatc
    34501 taaaagagga tttcttattt ctaatcccat attcaatcta tttatagaag gccctaagtg
    34561 atttgctagg tctttaaata acctctcgtc attttttaaa cttattttaa attcatcttc
    34621 cgccaaatct atcatcttat ttgtaatttc cattatttct atatcatcta agtttaaagc
    34681 cttatggttt gaagatgacc tttgctttgc acctcttata tgcatagtta tatatccaac
    34741 ctcatctact ggtatatcca tgttaaaatc tgtttccata ccttttacga tttctttagc
    34801 taatttaaac tcttctgtta tagataattc ttttagaaac tctttatcca tagttatatt
    34861 ttcaccattt tttatcctct gtaaggctaa tgaaatatga actacaagcc ctatatatgc
    34921 actatcagct aaatttacat ctaaatctga aagcttcttt gttaatgtag cttcaacttt
    34981 tcttattatg cacctgtcca ttaaatttaa aagtctattt tcacttaaaa tttctattgc
    35041 tttatctgtt tgtatatttt cacttatatt tctgaccata tctagtattt ctttttcatt
    35101 gaatgaatcg tatataagat ttacatatgc ctctctaaag tttttttctt gaccttttaa
    35161 gtagacacca agccctggtt ttctaacaag ctcaatatca aatctttcca accatttact
    35221 tgctaaggca aaatcattat tcagtacccc ttcagatacc tttaatattt ttgcaaaata
    35281 ataggatttt actggctcgt tagaaactag tagctttgat aatataatta aatttctttc
    35341 ttccttgctg tattcttttt ctattttttc ttcttctaac aactcaatta taaagttttg
    35401 attttctata ttttcctcta aaatcaatcc cactcctggt ttttttacaa agttaaaatc
    35461 attttcatca aaccattttt caattccaga catatctcta agtacagttc ttgaacttag
    35521 ctctaaagtc cctgcaatct cagaaatagt tattggaatg tttttctttg aattttctac
    35581 tatcattagt attatctttt tttgcctaga tgtaatcttc ttttttttca aattatccac
    35641 tcctaattag tggtactttt tatttttatt tagattctaa tcttttaaat aatgcatcta
    35701 aatttgggtc atttaaaaaa ttgtttattg aaactatctc aacacttgaa ttattcttag
    35761 caactctttc aactaatgta ttgtgagtta ctactatttg tgtatcatct ggaagattat
    35821 caacagatga atttgtaatt tctatattta agtctaagtt ttttattcta tttttaaatc
    35881 tggaagctcc cattgcactc gaacccattc ctgcatcaca tgcaaataca atcttcttaa
    35941 tctcatttac ttcaagctgt ttttcctcaa tatttttctc agagttctta cttgcagatt
    36001 tcatatcact catttttgct ttagcttctt ctaaagaagt actgtcttct tcactttgat
    36061 ttgctgaagc tctcttaaca aatggtgctg ccactaagaa tgatacaact gttgccactg
    36121 caactcctgc taatactgga agtaatcctc cctttggagc tagtgccata agtgcaaata
    36181 tacttcctgg tgatggtgct gctacaagcc cagctcctaa tattgaaaaa gtcaatattc
    36241 ctgctgctcc acctgccatt gtagccaata ttaaaactgg attcattaaa atatatggga
    36301 agtaaatttc atggatacca ccaaagaaat gtattattac agctcctggt gctgattgtt
    36361 ttacacttcc tctactaaac atccaatatg ctaatagaac tcctaaacct ggtccagggt
    36421 tactttctag taagaacatt atagatttac ctgcttcttt tgcctcagct atacctatag
    36481 gccctaatat accatgattt attgcattgt ttaagaataa tacttttcca ggttctataa
    36541 atactgaaac tagaggcaat aaacttttgc taactataaa ttgaactcca gattctataa
    36601 gagctgtccc tgctactatt gctggtccaa ttgcataaaa tcctagtatt gcaagaagca
    36661 tacctattat acctattgaa aagttattta ctaacatttc aaatcctgtt ggtatctttc
    36721 catctacaaa tttatcaaac ttcttaatta cccatcctgc aaatggtccc attatcattg
    36781 ctccgataaa cattggtata tctgcaccaa caattactcc catagctgct gctgcaccta
    36841 ttacgccacc tctttgtcct gctattgctt taccaccagt ataagctatc aatagtggta
    36901 aaaggtattt tatcattggg tcgcctatag tactcaattt ttcattaggc atccatcctg
    36961 ttggtataaa taatgctgtt ataagacccc atgctataaa agctcctata tttggcatta
    37021 ccataccact taaaaatttc cctactcgct gtatagatgt ctttattgtc gattgattag
    37081 aactactcat aattttacct cctcttgaat tatattctat atcgatatga ttcccttttg
    37141 ttatactttt attatagagt ttatatttta tagtaacaac tttaagaaac cgtttctatg
    37201 tcactttaga taatgacatt tgtcatttta gtttttgttt aattttattt atgtagtatt
    37261 ttggagtata tgattttgta aataatacta tttttataaa atatttaaat ttaatagaaa
    37321 tgaaaataat aattcagtta aataaattta ttaaatgtac ttttggatat actaatatag
    37381 atttaattta tctgtatttt tgaggtttat gcactctcta gtaatattat caatataaac
    37441 tatacaaatg taattatatg aatcactaaa gattaatttt aaattagcta ctattaaaca
    37501 ctaaacatta tttaatatac aactttaccc tataaggcaa tatatattaa acaaatttaa
    37561 ttaaaatagt ttaaattttt caaaaatctc aatatttgac aaaatacttt aataaaatta
    37621 aagaataagt tataattaat actatcaatt ataaaaagta taaaaatata aggagtgatt
    37681 aaaaaatggc gttttatttt gatacaccat cacatacttt cagtgaatac cttcttgtac
    37741 caggtttctc ttctactgat tgtatacctg ctaatgtaag tttaaaaaca cctgttacta
    37801 aatttaaaaa gggcgaggaa gcagatattt atatgaatat acctttgact tctgctatta
    37861 tgcaatctgt ttctgatgac aaaatggcta ttgcacttgc taaagaaggt ggtatttcat
    37921 ttatatacgg ttctcaaact attgaaaatg aagctgctat ggtagcacgt gttaaatccc
    37981 acaaagctgg ttttgttgta agtgattcaa atattaaacc tgataacaca ttaaaagaca
    38041 tacttgactt aaaagaaaaa actggacatt ctacagtagc tgttactgaa gatggaactt
    38101 caactggtaa attattgggt attgttgcaa gtcgtgatta tcgtataagt cgtatggatt
    38161 tagatactaa agttagtgaa tttatgactc ctatgtcatc tatagtttat gctaacaaag
    38221 atgttacttt aaaggaagca aacaacatta tatgggacca caaacttaat tctcttcctg
    38281 tacttgatga taatggtaat cttatgtata tggtattccg taaagattac tcttctcata
    38341 aagaaaatcc acttgaactt ttagattctt ctaagagata tgtagttgga gctggtatta
    38401 atactagaga ctttgctgaa cgtgttcctg ctttagttga agctggtgct gatgttttat
    38461 gtatagattc ttcagaaggt ttttctgagt ggcaaaaaat aacacttgat ttcattcgtg
    38521 aaaaatatgg tgatacagtt aaggtaggag ctggaaatgt agttgaccgc gagggattcc
    38581 tttacctagc tgaagctggt gctgacttcg ttaaagttgg tattggtggt ggttctatat
    38641 gtataactcg tgaacaaaaa ggtataggac gtggacaagc tacttctata attgaagttg
    38701 ctcaagctag agacgaatac tttgaaaaaa caggtattta tattccaatt tgctctgatg
    38761 gtggtatcgt ttatgaccat catataactt tagctttagc tatgggtgca gattttataa
    38821 tgcttggtag atacttctct cgttttgatg agagtccaac taacaaagtt aatataaatg
    38881 gtagttatat gaaagagtat tggggagaag gttctgctcg tgctagaaac tggcaaagat
    38941 atgaccttgg tggagataag aaactttctt ttgaagaagg tgttgactct tatgtgcctt
    39001 atgctggttc tttgaaagac aatgtaactc tttctttaag taaagttcgt tctactatgt
    39061 gtaactgtgg agcacttagc attcctgaac ttcaaaaaaa tgctaaactt acacttgtat
    39121 cttctactag tatagttgaa ggtggagctc atgatgttat gttaaaagat aatagaaatt
    39181 tataatataa atatacttta atcatttaaa caatgaaaaa gagacttatc tctattttga
    39241 gacagtctct ttttatatta gtataatttt tatttaactg taaattcaag aagcttactt
    39301 ttaagttcat tttctatttt tgataattca acttctgctt cttgtctctt tattctacca
    39361 tcatgttgta ttttaactac ctcatcaata gtagatatta gagattgatt agttttctta
    39421 attgtgtcta tatctacaat tcctctttct gattcttttg ctgtttctat tgttcccatc
    39481 ttcaaagttt ctgcattctt caataaaagc tcatttgtca tattactaac ttcacgctgt
    39541 gctttcatag cttgatttaa gtgagaaatt ccaagagcaa gcactatttg atttttccat
    39601 aatggaatgg tatttactat agttgattga atcttttctg ccatcagatt attactattt
    39661 tgaaccaatc ttatctgagg agccatttga agcgatacca ttctagttaa ttctaagtca
    39721 tgaattttct tttcaaatct gtcacaaagt gaaaccatat catttacaga ttgagcatct
    39781 tctgttgaac cagataaacg agctttttct ctcaactttg gcatttcata ttctttcata
    39841 tcctttaatt tttgttttcc tgctataata tacatagtaa gctcttttga gtaagctaag
    39901 ttcaattcat acatcttatc aagcattgct atatctttca atattttaac ctgatgcttt
    39961 tgaagctctt tacttacttt atccacattt acctctgcac tatcatactt agtttttaaa
    40021 tttgaaattt tattatttgc ctttttaaaa attcctaaaa aaccactttg ttctttttca
    40081 tctgctgaaa atgcttttaa atcagtaact agatttgtaa gcatatctcc aacttctcct
    40141 aaatctcttg tttttacact ctttaaagct gtttcagaaa aacttgctac ctttttttga
    40201 gcaccagctc catattgcaa aatcatattt gtatttgtaa tatctatttt ttccgcaaat
    40261 tcctttacca tcttgcgttc agattcagac agtttttctt cttctgggtc tttttcatcc
    40321 tttttcatta ataagtcaga attatctata tcattagtgt ttgattcttc tccaaatggg
    40381 tcaagtgtca aagttggcat aactggtata tcatctaaat tattcattgt atctttctct
    40441 cctcttcctt cataaagtac tgattaactt atataataca cattttatac tttatgacta
    40501 attaattgtt taaaaataac agaatatttt ttctattgaa gttaaaattt accactcgtt
    40561 aatccttctt ggactaacat attctctaaa acagtaatat ctgtagaaat atcaatggct
    40621 tcatcttcaa acagatagtc caattgattt tcaaaagcta ctacaatcgt attcattgta
    40681 ttttcaatac tttccatagt tgttgttata ttttctctat caattccttg ttcttccaat
    40741 gtatgatatg aatttaatag ttttaaagtg gttggaagat aataatccat aaatttttgt
    40801 atttgagtaa gttttgatgg gtcatgtttt acaacatcaa atattttact tgtaacatct
    40861 tctacttgta caattttatc gcccattgat tctgatttaa tatttttatt agcttctttt
    40921 atttgtgtta gataattcat tcctttattt ataattgtat caacttcatt ggattcatca
    40981 gtcttttctt cttctttgac ttcatatgct acatcttcat atcttggtgg aataaaatct
    41041 tcatttgtta acacaatctg attatttctt ttatcaatat aagtatttat aaagtagttt
    41101 ttgtctatca tcttttgtac atcttttatt ataaaattta atgatttaga tgtgttttct
    41161 gcaataagtt ctactggaat aatatttcta ttgtttataa ttgtacaata ttttttataa
    41221 cgagaaattt ggtctcctaa tctcttacct ttaaaaatac aaactcctcc tataagtaaa
    41281 aatactgcac atacttgaat aggaagtatt acagcccatc caattataat ggaaatagtt
    41341 gaaagtacta aggctgataa tgtaaataag cttaccaaac cccatccaat tatttttaaa
    41401 aatttactta aaaatgaatt tattaataca gatttttttg aaaaattttc tgatttatac
    41461 atattttata atcctttcat tttcactaat attattaata tattgcagtt tgattataca
    41521 attacttact tattactgtc aaggaatttg aaattctaca aattgtttca aataaagtaa
    41581 tagacttaaa tataagcata ttttaaatta aaaatatact tatatttaac tccatataaa
    41641 aataagagaa cttaacttta attaaaattt aagttccctt atttttattt gtacataaat
    41701 ataattagca ttgtttatgt tgactataca tataaataaa aaattgtatt aataaagatt
    41761 tgcaaatatc tctcttattt cctctctact aagctctaca taattattag caagtagtcg
    41821 ttgttgattt gctattgtac tatctgtaaa ttcatctatc tgactttcca ccattccata
    41881 ttctctcaaa gcttttttag ctattaattt atttaaaaac ttttctaatt ctccataaac
    41941 atcacagtta gggtcacatt caagggcatc tgctaaaatt tttgtacatt ttataatttt
    42001 tccattaggt ttttttctag aatacatctt aaatacttca gtaaagaact gataatttgc
    42061 ttcaccatgt gcaacatgga aagctccacc tattgaataa gatagtgcat gaactgctgc
    42121 acaaccagca tttccaaaag caattcctgc ataatttgat gcaagtacaa agtctctaag
    42181 atgattaaat ctctcttctt ctcctttatc aactatcttc ttatacccat ccataatcat
    42241 ttttattgct tgtagagaat acatctctgt aaatggactt gcttttggag aaagataaga
    42301 ctcaatagca tgtataagag catctaccga acttgtaaca aagaatttgt aaggtaaccc
    42361 ttttactgtc tctggaatta acactgcata gtctgcatat gtttcttcta ctgcaagccc
    42421 ctttttagta tgtttagatt ttaactctgc aatagctaca tttgttactt cactacctgt
    42481 accacatgtt gtaggaacta ctactaactc ttttactttt ttaggtgata cttctccttc
    42541 aaataaatca atagactttt ctggaacatc aagtgctaat atcttacata tatcaactat
    42601 tgttcctcct ccaaacgcaa taattctatc aaaattgtat ttcttcattt cctttgtcat
    42661 ggagtctatc atttcatcag atggttcacc tgttccaaac ttttcttgaa aaattaaatt
    42721 tgtatctatt ccaagaggtt tcatatatgg ctcatatata aactcattag taattactaa
    42781 atcatttttt cctattttaa attcttcatt aaattcttta aatgtatcaa aataaaatat
    42841 ctttggaact actctaagtg cttccattat taacacccct ctattattaa tatttctcct
    42901 taaatctctt ttcaaattct actgctagac tttctcttac acttggatga gctatattta
    42961 ttaaatttct tgctctttct ctaagagatt ttccttttaa ttctgctata ccgtattctg
    43021 ttacaatata gtcaacatca tttcttgatg tcgttatagg tgcaccttca tccacaatag
    43081 atacgatttt tgaaattact gtaccatctt tttttgtagt tattgaaggc atagctataa
    43141 ttgcctttcc atcttccccc atagacactc cacgtataaa gtcaacttga cctccaacac
    43201 cactaaattg tctaagtccc atagcctctg caactacctg tcccattaaa tcaacttgta
    43261 tagcagaatt tatagaaacc atcttatgtt gtttcattat aattgctgga tgattcacat
    43321 agtctactgg ttttacctct actgcagggt tattatttgc aaagtcatat aattttttag
    43381 tacccattaa gaaggtaact gtcatttttt ctttatcaaa atttttatat ttaccattta
    43441 taacaccttt ttcataaagg gccaaagtcc catctgaaat catttctgaa tgaatcccta
    43501 aatccttttt atcagttaaa aataacataa ctgcatctgg tatcccacca atacctagct
    43561 gtaaagtaga accatcttca attaatgaag cacaattttt accaattgct tcttctactt
    43621 ctccaatctt tgcaggttgt agttcaaata aaggttgtga tttttccact atatagtcaa
    43681 actctgatac atggactacc tcaccatagg ttctaggaaa ctggtcattc acttgaacaa
    43741 ttactgttct agcagtttta atggcttcat aagtataatc aactgatgtt cctaagctac
    43801 aatacccatg ttcatctggt ggagttacct gtgctaaaac aacatcacat tttaatcttt
    43861 tttctctaag caactttggt acttcatgga agaatgcagg tgtaaaatca ccatctccat
    43921 tttcaattgc ctttcttaca tttccactta caaacattgg atttactcta aaatgagctt
    43981 ccattccctt agcagtatat ccaccactac ccaagcttac catatgttta atctcaacat
    44041 ctttatattg ttcagcattt tcaatcatag cctctactag tgcaggaggt tcacctacac
    44101 aatgtgcaaa tactacagta tcaccgtttt taatctgttt tacagcttct gtagcactac
    44161 ataatttact ttgatacaat tcttgccaac tcaatgaaat ccccccattt agctttttta
    44221 agtttcacaa attgctgatt caatcacatt tataactctt tctatatggc taaaatccca
    44281 catcttaaca cattcatttt tcactttata ttgttgatag gatgcgcata gagttgcgca
    44341 tcctccaatt ataagtagca aatcatactc gacgttctct tgtgctatgc taaaatctac
    44401 cctaccattg aagtgctctt tgattattgt aagagccttc cctctttcgt atctagggtt
    44461 acagccccca caaaatctaa ctccacaggt catgtgtaaa ttctccttat taaaaatttc
    44521 tttacttagc taaaaaccac cataatttta taactatctc ttatactaca tatcttttca
    44581 ttaattaata cacattaata tataatttcc ctttatatat caacttttta taaatttaaa
    44641 gcttcctctt tttttattcc cgcaattttc ttagctaatt ctttcttttg atttatgttt
    44701 ccttgtcttg aaatcattat tctttgagct tgtggtgaac ctgccccatg cattgactca
    44761 gttctataac caacagcaga tgaaccaaga catatatttt ctaagaatct taatattctc
    44821 attctttctt ctgttgaagc cactgaagaa gctttaaaat acttgttaca tacttcgcca
    44881 atagtcattc cacttgttcc aactttaagg tctgatttaa agtctttttc tgaaggcata
    44941 gtaaccataa gccctccagc tatatcttct gcaagtctta ctatttcata aggaaatcta
    45001 gttacatttt gtttacacac atttgcaaga agtaaatcta tttgatagtt tccagcttct
    45061 gttttgtgac cttctgatga acatgctata ccacagcaat ataagctttc attaaggtga
    45121 gtcatttcta ttagtttatc ttttatatgt gaagctttgt ttgctccatt ataatcagct
    45181 gctaaagctg ctgctcctat tattacatca cctactccta ctttacatcc accataactt
    45241 tgtctatggt atcctgcaaa tctctctact aacataccag agaaatccca ttctccacat
    45301 aagaaaattc tatcatttgg aacaaataca ttatcaaata ctactaaagc ttcttgacct
    45361 ccaaactcct tatttcctaa atcaacatct gcaccttctt ctaattttct ggtgtcacaa
    45421 gattgtcttc cataaatcat aaacacacct tcagcatctg aaggaactgc aaatgataca
    45481 gcgtagtctt tatctgcttc agtcatactt atagtaggca ttataaggtg ctcatgtgaa
    45541 tttattgaac cagtttgatg tgcctttgct cctcttacaa ttatcccatc ttctcttctt
    45601 tctactatat gaaggaacat atctgggtca ggttgagcac taggtgatag acttctatct
    45661 cccttagggt cagtcatagc tccatcaact actaaatcgt tttcttgtat ataagttaag
    45721 tattttacaa aattatcatg ataattagtt ccatgggctt tatcacactc aaatgttgtt
    45781 gaatacactg cattaaacgc atccatacca acacatcttt ggaaacaaga agctgttttt
    45841 tgaccacata gtctttgcat ttttactttt tttatcaaat catcaacact ttgatgaaga
    45901 tgaccaaatc tatttatttt ttcacctgtg atatttgaag taactgtcat taaatctgca
    45961 tattctgggt catgagctaa gtcataagtt gctgctacac aatttataga tggtctaatc
    46021 ataggatggt caacccaatt cttaacctct tcaccaaaca tataaacctt agtatttaat
    46081 tttctcaaac tttcaatata ttgagctcct gtcattaatg ccataataaa accactcctt
    46141 ctcttttata gattttttta tttattttaa attataatac ttccttcttc tatatgtctc
    46201 ttaatatttt aaatttagtc caataaacta atttaaattt aatttatgtc ttaaattcat
    46261 tctaaattaa aatatattcc tactttaatt tagaatgaat attttttgtc taagttttat
    46321 ccccaaagtt cttcatctgt agggacttca ttttctggca tataataagc aattctagaa
    46381 atattaatta gatgcttata atcaagattt tcagagatgc tgttatttcc ccaagaacca
    46441 caacctagag tattagtagg tgcaagacca ttaaagaaac ttcctccagc actagtagca
    46501 caacattgat taataacaaa tcttgatact tctatatttt ctcctgcata ttctatattt
    46561 ttaactgtat ttgaatgtat agatacacta tgtccttttc cttctacatt taaatttgct
    46621 tttgcaatag ctacaccttc ttcaaaactt ttatacttgt atgcagatat aactggacac
    46681 attttttctt ttgcaataat atcctcttct cctggtccat cagcttctat tactattatt
    46741 tttgtatctt ctggtatctc aacacctgca atttttgcaa ttgtatgaac tgattgacct
    46801 actgcatctt tattcataga tttatttaca aacatagcat ttctgaaagc ttctttttgt
    46861 actttatctc taactataaa acctttgtta tttttaaatt catccattat tttatcaaac
    46921 atttcttctg ctactattac tgactgttca cctgaacaaa tgataccatt atcaaaaatt
    46981 cttcctgcta tgattttagg tactgcttct ttaatgtcta catctctatc tatgatacat
    47041 tgaacatttc ctgcccctac accaagtgct ggttttccac ttgagtacgc agcttttacc
    47101 attcccattc ctccagtggc aattactaca tctgctgatg aaattaaatt tcttgtattt
    47161 tctcttgatt gttggtcaag tatttgtatt aaattttctg gagctcctat tttttctaat
    47221 tcttcattaa tcatctctac agtttttgta ctacatccaa ttgctttatg atgaggtgtg
    47281 ataataattg catttcttcc cttaagtgca aacattgcat tgctcatagg tgtaacgatt
    47341 ggatttgtac aaggagttat tgccgctact actccaactg gtttagctac ctttgttatt
    47401 ccagtttctc tatctatatc aattatacca actgattttt tatcttttaa attgttatat
    47461 ataacttttg ctttgctttt atttttagct accttatctt catatactcc cattccagtc
    47521 tcttctacag caagtttagc taaatattca gcattataat acactacttt tccaactatt
    47581 tttactattt tatctacttg ttcttgagta taacattcaa actctctttg agcttttctt
    47641 gctctctcaa tataaccatt aatgtattcc ttacttaaat cttcaaagtt ttccactgct
    47701 ttttccattt aaaatctacc tccaatttat taaatatttt ataatgcact tttaaaagtt
    47761 tcagcctttt tcattgtacc tgtactcata aatttactat gccatgataa agcttcgtcc
    47821 aatatgtgag gtgtttgttt tgctccactt ttacaagctc tatttaaata atccctaagt
    47881 tgttctttat aatctggatg ggcacaattt tctattattt ttatagctct ttctctaggt
    47941 gcaagacctc ttaaatcagc atatccttgt tctgttacaa ttaccattac atcatgttca
    48001 gtgtggtcta cgtgagatac cataggtact attgatgata tatcaccatt ttttgcaatc
    48061 gactcagtac taaatatagt tattgctcca tttctagcaa aatcaccaga acctccaatt
    48121 ccattcatca tctttgaccc cattatgtga gtagaattta catttccata aatatcaact
    48181 tcaattgcag tgttcattgc aattacacca attcttctgg ctatttctgg attgttacta
    48241 atttcttgag gtcttaatat aatcttatcc ttaaaaaaat caatatcttt ttcaaatttt
    48301 attaatcctt ctggagatgg acttacagat gtagtagatg ccattgtaac tttacccatt
    48361 cttattaaat ccaacataga gtcctgtaca acctctgtat aacaagttaa attttcaaat
    48421 tcagattcac ataatccata taatactgca tttgctacac ttcctacacc agattgaaga
    48481 ggaagtaaat tctttgataa tctgcctgcc tttacttctt ctcttaaaaa agcaataata
    48541 ttatttgata tttttttcga agcttcatct acaaccccca atggacgggt cttatcttgc
    48601 atattggtaa ttactactcc tgcaatttta tccaaaccac aaggtatata tgttgttcca
    48661 attctatcct taggatgatt tatttctatt ggttttctat ttggtggatt ttccgtaata
    48721 tatatatctg ccataccttc catttccatt ggttttgcaa gatttatctc tactattact
    48781 ttatctgcac tttttataaa agatggagaa ttacctatcc cactagttgg tataatattc
    48841 ccttcttcag ttattgcaag tgcttctact atagctacat ctactttagg tatagttcca
    48901 taatttaaaa attgagtaga atgacttagg tgcatatcta tataatcaac aacaccttta
    48961 ttaatattat ttctaagtat tgaattagtt tgataaggaa gtcttctttc aattatacct
    49021 gcttctgacc atgctccatc aacttctggg cctaaagatg ctcctgaata tacggttaat
    49081 tttatctttt ctccactttt agctctttct gaaactgcta atggtactgc ttttggatag
    49141 cctgaaggtg taaatccact aactccaata accatgccat cctctataat tttagcagct
    49201 tcatctgcat tcattattaa atcacacaat tctttatttc ttaatcttcc acttaattct
    49261 tcaaatgctc cttcattatt cataaatatc cccccatata aaatcttatt tacattggaa
    49321 gtttgtgtaa aactataacc aatataactt tagttaataa tgcaaatgga tacgtagcac
    49381 cataacctgc tgctgggtca tcactatcaa gagcatcaac agcagctcca agacctggtg
    49441 ttgatgtcat accaccacag acagctccag ataataaaat ccaatttaat ttaaatacgt
    49501 atttgccaac taagaatcct atcatcatag ctgtaacacc tactgctatt gctactaagg
    49561 ctaggtgcat accagatgtc ataattgaat caactacttt tccaccatat cttaatccaa
    49621 ctattgctaa gaaaaatact agccctattt gttttaaaat atttaaaacc ttttcttcca
    49681 ttctgaaatt tactacccct atcttaccta tatatccaag aactagagat acaattagtg
    49741 aacctcctgt tgcgcctagt gtgaagtacc ctagtggtcc cataaataca tgtattcctc
    49801 caactaagta tccagctaaa catgtcaaga aaaatgttat tatattaaat ggaacttctg
    49861 gtatcgtttt accagaaaca ctatctcttg cttccttcat ttccttctca tattttcttc
    49921 tttcttcttt taggtccatc ctaaataatt ttggaaggaa atttacacct aagattacta
    49981 ttaaaactcc aaaaggatat gcgactgcat gacctatacc aacaccagct tcagcatatg
    50041 caatatactt atctatttgc tcttgagtca gtgatgtagt tgtattcaca tccaatttgc
    50101 cttctgggtc gactactttt aaaatttctt ttttatcttt tattgaagct ttttcaaatt
    50161 cttttgatac atcttctgca tgtttaccag ctgtttctaa agcagcagct aatccaggcg
    50221 aacttgttaa tgctcctgtg taaacgccag aaacttcata agcactactt cctttatcac
    50281 ttgatagtaa agtcattgca taagtcatag agccacctat aaaagttata agaactccta
    50341 aaattacaaa tcttgctcca tacttcttta atacagcttt catatctttt gctgccaata
    50401 gaccaactgc tgcaacaaat attattaaaa agaaatcaaa gaagtcagaa gaaataattc
    50461 cattccccat cattacagtt gctgccttat atcctgctgc tgtttctcct tttgctatta
    50521 ttagattccc caagctatat gcaagccatc caataaataa tcctgtaaat aatgctcctg
    50581 ataccccaaa attaaacttc ccaaatttaa tttttccaaa taaaattcct gtaattactg
    50641 cagcaaacat aaggacaaaa ggattcatta aaaaatccat caattcaaac ttcactaaat
    50701 cactcccttt catttttcgt tattaaatta acaaatatta ttaaaaaata tatttctcct
    50761 ttctccccaa tactctattt tgttattaaa ttaacatttc tgattaaaaa aattttatct
    50821 tgttaatatc acttttttct ttatgattta attaacaatt taattgtaaa aataattttt
    50881 attttctaat agataatttt ttgatattcg ttgatataac tgtaatatag attgatttac
    50941 tctaaaccat accagctatt ctcttttgtt aatttaataa tagcactatt cttaatagat
    51001 tgaaaatatc aaaaacttat gctatgatag tttaaaacta ttctattttt ttacaacaat
    51061 atacaaaact tatgctattg ttaattatat acttgtcact aatatgtaaa tgaaaggaac
    51121 taataccaaa tgaatataaa ctatttatac tattttcaaa ctgtctgtaa atataaaaac
    51181 atgactaagg ctgcggaaag tattcatatt tctcaaccat ccataacatt agcaattaaa
    51241 gaattagaaa aagaacttgg ctttgaactt ttttacagaa taggtaataa aatagaatta
    51301 acgcctgaag gaaaaatttt tttagacaag tcaaaacatt ttataaaaca atttgaggat
    51361 tttcaatgtg acgcacttga tttaggaaaa aagagaaaag cttctctcaa aataggtatt
    51421 cctacagttt taggtacgtt tttgctttca aaaatacttc ctagatttaa tgtaatttat
    51481 ccagatatag agcttaaaat ttttgaagtt ccaacatttg ttggagctaa aatgattgaa
    51541 gaatctacac ttgatttttg tattggtata attgacagtg atatttatga tgatatagat
    51601 tcaaagacaa tctataaaac agaattgtac ttagttacaa atcccaaaaa tgaattggca
    51661 aaacatccca ttatatcaaa ttatatgtta aaaaatgtac cttttgtaat cttatctgaa
    51721 ggttcttatc actataagat tattacaaaa agacttgaaa aagctaagcc aaatataatt
    51781 ttacactcaa atcaactttc taccatacgt tatttactag aaaatgactt ggcttcaaca
    51841 atattgtata aagaaatatt tcaaaataca gaaaatctct gttcaatacc attagaacgt
    51901 gcaattactg caaatattgg agttctttgg agaagaaatc aatacatatc tcatagtatg
    51961 aaacttttta ttgagtatat ggcatctatt catataaact aaatttatat tatacagata
    52021 taatattttg tttaaataca atgttaaaat attttaataa atagtatagt ttagagtatc
    52081 agtataaaaa tacaatatag aatgttatag aattataatt gtatacattt aaatctaaat
    52141 attattatac tgatactttt taaaatacct tttattgata atactttttt aatcaggttt
    52201 acttggacaa cctagcaaaa aacccacgca tcctgagaca aaatctctca tctgtgaaat
    52261 agtacctaaa gaagctgatg ttgtagttgt aaaatttctt acaacaaaaa gtactgttaa
    52321 tgctgctata caaattacag tagctataat aatttgcaat tcactagttt ctccttcttc
    52381 aaaaccttca ttattattaa taggtatttt cttttcataa ttatcagctt ctgaagtaaa
    52441 tccaatggta tttttgacat tcattatatt attttgacta gactcttgac cttttggggg
    52501 taaattatta tctataaaaa gatttgaaga tacatcagta tttgtatact tccttaaagg
    52561 tctctttata aaattagcac ttaaaaaata cccaaatata gctgctgaag tggttcttac
    52621 tactacatca atagttgtag tatcttgtga atttacttca ttaacaaata aattatatat
    52681 agattgaaac attaatattg ccattacaat tataaggcat ctatcaatta atttgatttt
    52741 ataccaggct ttttttatat tttttataaa tttatgtttt ttatacatat atactatcac
    52801 ctcttacaat aaaattaagc cctctttact ataatatgta aatagagctt aatagtgaga
    52861 ttatgttata ttttatgtaa aaaaatatat ataaattaaa ttatttgtct aaaatcatca
    52921 aatctttagg gaatttagta agtctttctg gaccattttt agtaatcaaa acagtatctt
    52981 ctattctaac tccaccccat ccagggaaat aaatacctgg ttccattgtt attatacatc
    53041 cctcttctat gattttatca ccataattac caatgaaagg ttcttcatga acatccctac
    53101 cgactccatg tcctatacct tgatagtaat agtcttcata ttttttgaca acttttctta
    53161 tctcagcatc tggaattgtc gcatgtacac cagctttcat gttctcaacc cctacatttt
    53221 gagcttcctt aactaaatta tatatttcta attgcttttc agaagctcct ccaactataa
    53281 aagttctagt tgtatcagat atatagccat tatacatagc accatagtct ataagtacaa
    53341 aatcaccttt ttctataatc ttatcactag gcttaccatg aagcaatgaa gtctttgctc
    53401 ctgatatcag tatagtttca aaccctatat tttgtgctcc gttcattttc ataaagtact
    53461 ctaactttgt agccagttca atttcgctaa ctccagcttt tatatgtgga atcaattctt
    53521 ctaatgcctt atccgctatt tcacaagctt ttcttgtatt tttgatttca tctttgtctt
    53581 ttacatatct caaagtctct acaatatttt ctgtaggtat taattctcca ttatccttct
    53641 caatcagatt tttaagttct tcatatttat caaacactat attagttctt tcaaacccaa
    53701 gtttctttat acccacttta tcacatacac ttttaactgc ctttgccaca cttctatcaa
    53761 aattatgcca atttataatt tcaaaatctt tgcaaacctt ttctgcaagc tccatatatc
    53821 tactatcagt aactaaaaag tttccatctt tgcaaatcac tgcatatgct tcttcatcag
    53881 gaaaaccact tatatagttt acattagttt tctttgttag atatagtgca tcaacacctt
    53941 tagttcccaa caatcctact acatttttta ctctgctcat gattttacct ccaaataata
    54001 aattttatct taattatatt atatcactaa ttaatcttat ttttatctaa cagatacttt
    54061 attcctaaaa ttttttcata ttttattaat ttcattttaa tacttcatta gtttttattt
    54121 agttagaaag cttaatttta attgaataaa tataataaat tagctagaga atcaaataaa
    54181 tggattgtgt taatatataa atattgacaa gatataaaat aaaaagaaca tataattata
    54241 atcaataaaa ttataactat atgttcttat ttacatataa aaaatgtact tattttgcag
    54301 tatatccagc tttaactaaa gtttcaactg ctaagtctcc tttagcttca tttactaaaa
    54361 ctattggacc tgtacaaccc ataccacttt cagcatagat tccgtttttc cataactcag
    54421 atactgcatc ttctaaatcc attatatcaa caccagctat ttgacgagta acaatttctt
    54481 tatctggcat tttaacttct actttttcag ctttaacttc ttttttagta agagaggata
    54541 taatgtcatc aaattttgct tttttaacct ttgcaaattc atttcttgca acattactta
    54601 tatctccctt tacaacatca taagcgtatt ttaaagcatt tgctactacc ggagaacctg
    54661 atgctcttga tactataagt acctttctat catagtcttc tccaactcca ggtccgtatc
    54721 caaagccttg agcttcataa tctccacctg tagtatatga agaaaatact ttcataaata
    54781 tatttccaga taatgtatca gtaaccataa catcaggtgc acctacaagt aaatcattac
    54841 ctctcattac acatccacca tctgctctaa ttgaatccgc aaactccatg tcatatccat
    54901 tatcttttaa ttcttttaag cacttctcaa cttgtcttgc tccatcaaca ttaagtattc
    54961 caacttttgg gcatttgtta cccattgatt tagctgtagc aataccataa atggcatttc
    55021 ttaccattgc ctctattcta ttagtagcac ttgttcctgt tgttgttgca agtatcattt
    55081 cttttccttt tgctggagtt attactctac ctactgtaga tacacctatt ggaaaattat
    55141 aatgcatagt aacacaagca tcaatatatc ctgaatctaa taattcttcc atttttgcat
    55201 gcatttcttt ttcatcatta acttcaacta cttcaaggtc agtttccact tttggtccaa
    55261 taagcacaat atcaaataaa ttagattttg ctaattgtgc accattaacc atgttttcta
    55321 caccatgttc acttccaagt gtagtaacac caatttttac ttttttgcca aattcgccgc
    55381 tttctattgc attagctact tctaagaata catctgcgat tacttttttt gacatatcct
    55441 cacccctatt cttctaacat atctaatgct agttttttca tagattcagc tattatcttc
    55501 ttaatttcat ccttagatat tcctgaagtc tcttcagtaa cacctgtatt tctttcagct
    55561 ataaaagata ctccatcaaa taagttagtc attcttccta agaataaact accttttcca
    55621 actatcatag ctctatttct atttcctgta gttaaatcat ctattaaaaa tcctatataa
    55681 ggagctcctg atggtatatg tccttgagtt ggtgcccatc ctggtaatcc tttgttagaa
    55741 acaaaatctt tcaattcttt cttttctaag tctccacgtt taacagcaag tgcccctatc
    55801 attttgtagt ttgcttctgg aacatctcct gctcctgctg gcttagttat atctgggttt
    55861 tgcatttcaa ctgaatacac atcaacatct gttattttta aattagctct atcaagacct
    55921 gaagttataa gtgctgtcat aactgcttgt ggtgaagaac cagttccaac agtatgctta
    55981 cctgttaaat cagttcttat aactggattt acaccatcat tttcagatac aagtactgca
    56041 aatcctccaa caacatcttc aagtacagga agaccttttt taacatggtc tttagcattc
    56101 attccaagtt tagcacttgc tccacctgct actaccatta catttttatg tatgccagct
    56161 tttactaaag ctgctgcttg aattaatgca tgagttggtg ctgcacaaaa tcctcttgtg
    56221 tcagaaccag ttgcattctg aagtcctgct atctcagcta ttgatttagc aaagtttcct
    56281 ccacctcttt ggtttatatc tccacaagct tcttctgaac actcaataac ataatctatt
    56341 tcagcagggt ctattttgct atgtcttagc atttgaatag cagataaaac tccagatgct
    56401 tttactacta gattttcaaa cattgtatgg gcatttaagt taacatctac atcatgtgct
    56461 ctttttacat aaccaacaaa ttttccatca tggtataatc cttcagctat gtgtgttgct
    56521 actaattctt taccttcttc tatatcatca ccctttaatt tacctacaaa tggtgcaatt
    56581 tcagggtagt tattttcgat ttttggcttt acttcatcaa taaattcttg tgataatttt
    56641 actaaatcaa atgcatcact tatttgcatt aaagctatga actcatcttg aggcattatt
    56701 tctccaaact taccatttct agttccctca atcttttgtt cacaccatgg cattggtaaa
    56761 tctcttaatt cttctggtct tctatttcct atataaactt gatttggcat gtagttaact
    56821 acatcttcat aacttcttat atgattactt acttctttta aaaattctga atctgaattt
    56881 gttgctcttt caactgtaca agtacttcca ttttgcacta tcatatctgg agtatgaata
    56941 agtacatagc ctgccccttt taaaactgga taagtcattt tgtgcacctc cataaatttt
    57001 atgaacactt attttttaat cttattaaac ttattaaaag ctctaatcat tgtattttat
    57061 tgtatatttg attctattat tgtttatgta ttttggcatt ttttaagatt tcatgaatga
    57121 ataactccaa tttatccatt catgaaatct atattatttt ttttgtgtat agttattttt
    57181 tatttttagt gtgtgtttat atttttatct atataatata tgtgtgtgtt atttttattt
    57241 ttcaaatact gtttgaccat ctacttctgt agttaaagca tttaatgctt tttctacaag
    57301 gcctcttctt agggcttttt cttctgtagg gtcaagcgct ggatttccaa gtggatgagg
    57361 tattgctata gttggaacta ttctattagc ccctactgtt aatgaaatag gtactactgt
    57421 acacatatga actactggaa gtccagtttt ttctatttct tttaccatcg ttgcaccgca
    57481 acgagtacaa gtacctcatg tagaagttaa tattactgca tctacaccat cagccactaa
    57541 tgctgctcca atttcagaag catatttttt agagtttgca acagatgtac cattacctac
    57601 agttgtatag aaagtctcat gtaattttcc aattacacct tctttttcta aatctctaag
    57661 aacatcaaca ggtaatactc tatctgcatc taaatttgca tacactgggt catatccacc
    57721 atgtgcagtt tcataagtct cttctgttaa atccataact cctgctatag aataagttcc
    57781 atatttagaa gctgaagaag actctattct atctggattt ccttttggaa ctataccacc
    57841 agaagtaact aaagctattt tacattttga taaatctttt acagctttac ttgggtcaac
    57901 tctatcaaag tttggcattg ggtattcagt ttcaaatggt tcacctttta ttttcttaac
    57961 taacatgtca acagctctct tagaacctct gtcttcatgg aagtagttaa ctcttattcc
    58021 tctagctatg tatccttcat ctttaggagt tccaatttct tctccattag caagttttac
    58081 tgcaagttta gctatcttag gaagtgcctt tctcatacct gctgcagaat cagaagtttc
    58141 tactacataa acatcctttt tgaacatatc tgctcctgga ttttcaacat acatacctgt
    58201 taaagctggt atattcaaag catcttttac aactttagtt attgttccag cagcaacccc
    58261 atatctacca gcattaaatg caggaccagc tatgaaaagt tgtggttcaa aaccttttac
    58321 cattgataaa acttcttcac ttgcactatc aatattttca ttgaaatagc tatctccaca
    58381 aactactgtt ccaactattt caatatcttc acctaataat ttatcaagtt gtaaacttat
    58441 tggaggtaaa gtttctgcca catgtggctt agtatcagct ttttcttctc cacctatacc
    58501 agcaaagaac tgattaatat aatgtactgc ttttagtttt cccatctctt agtttcaccc
    58561 cttttgcttt taggcattaa aatttacaaa attcttctct tataccagac atttcttctt
    58621 ctatttcttc aacttctaaa accatttcca tcatacttat ttggtcttca tatacacttt
    58681 catcaaataa tgattttatt ggttcctcaa caacgtggta aacacttaat cctaaggcaa
    58741 ctccagcaag tggtccagcg aaagttggat ctccagcagt tactgtttcg gctgcaagac
    58801 ctgcagcttc ggcttcagca gcacctagta aaatcacaac attttcagct ccgaatttat
    58861 cagcagcatc tttaactctg ttttgatttt ctaagtccat agccccagca gctgttcaga
    58921 caaagcattc tgtagatgag aaaacaacct ctgctccttc tactgttttt acacattctt
    58981 ctatcgcagg tcctggtata ccatcacggt cacctattat aagaaccttt ttattactaa
    59041 gtaaactcat aataatcctc cttattatat ttctaatata tttttatata tctataacac
    59101 ttattataat tatctataat atgttttatt aacattactc aatcgtttaa taaaatactt
    59161 cttaatatcc tttagcagtt aaatgaccaa atcctgtttc attagttgca cctgttatag
    59221 cttggatttc aacttcaata gttccatcag ctcttaaact tccatcagaa ccaccagcta
    59281 ttacatctac tacttctaaa tgtccaataa ctttatctaa cttaggaaga actacaactt
    59341 ggttggcatt tccacctgtt acaacagcat cagctcttac atctgcatct gcaagtgatt
    59401 gagaacctcc atcacgtcca gcatattcat ctgtaactat aactgtttta acaccttgca
    59461 tttctatctt cttacagttc attataaggt cagtatcagg atttccaaaa ccttcttgtg
    59521 aaactataac tgcatctaat cctaaatatt tacataattt agaagtccag tttgaagatc
    59581 tttctttatc tgctaggtaa acattttcat tagttataat tacacctaca aagtttatat
    59641 ctttaccatg tcgtgcatat aagtcttcaa caaccccatt gtttatatga acataacttg
    59701 ggtttttgtc acatgcagaa acacagttac cacttacaat tgctccgtcc attaattctg
    59761 ttggatataa taaagttggt actatttgtt ttgcatctac tccatataca taagtatcat
    59821 gtaataaacc ttgagtctga agcatgtaaa catatcctac ttttggaagc tctggatatt
    59881 cttttattga ttcaagaaga ggtttagttt catatactga aacttcatct ggagtaagat
    59941 ttcttgctac ttctcctaag tacattgctg ctttgaatcc tatcattcta acagcttttt
    60001 catgagcgta ttgttttaaa ccatcaatag gttcagctat tactactaca ttatttaatt
    60061 tagaaaatgg agtatactta gctccttctc cagtcatgtc tattatacct tcttggaaac
    60121 ctactatttt tccagtagtt acaacagcta caccttttaa agcatgagtt tttccttctc
    60181 ctacagtatc aactttagac atgataccag ggaagattcc cccattacct tcaactttta
    60241 ctcttggctc aataacatct tttacaggtg ttattcttac gctttcacct ggacgagcta
    60301 attctatatc tatagattta atatgctcat ctccaccaat ttcttgtagc atctcttgct
    60361 tattgatgaa taatactcca tcttttactt ctgttacatc accaaattga atatctttta
    60421 taaagatttt ccctaattca agacgcatac ttgcacctcc tgttatttaa tttgtgtata
    60481 tgcatttgag tttttctcaa aattatatca ttttacaatt tatcttattt ttttaaagat
    60541 atattattgt gtttttatgc cttaaatctt tattttatta ctaaaaaata tttattgtaa
    60601 aaaaattatt ttatttaaaa ttattttttt aacaaagttg gacataaaaa cctttttctt
    60661 cttatcttca gataaaaata gattttttct tttaatgtta atactttatc aacatattaa
    60721 agatattttt taatcatgtt ttctatatta ggaactgtag catcatcctt agtaacttcg
    60781 tctacttttt ctccatcttt gtatatagcc atagttggaa gacctaatac tttttgtttt
    60841 atagctaatc ttctagcttt tgttgtatcc atcttacaga attttatttt atccccataa
    60901 gtctcagcta atttatgaac atctggcatt aaagctttac aaggctcaca accttcactc
    60961 caaaaatcaa caaatacaaa accttctgca tttaaaactt cttcttcaaa tgtagcctta
    61021 tctaaatcta acattttctt attccccctt gaaattttta ttttaatttt aaatattatt
    61081 cattaaattt acagacacta tttcttaatt tacagattct agttcccaaa tttttcatca
    61141 atgtatttct cagctactat agatgcaatt gcgccatcat tagttgcagt tacaacctgt
    61201 cttagagtct tagatctaca atctcctgct gcaaatacgc ctggtatgtt agttctcata
    61261 tcttcatcag ttattatgta acctttttca tccatatcaa ctttaccttt gaataaatca
    61321 gtttgagcat ctaatccaac gaacacaaat actcccattg ttccatcttc ttcatctgca
    61381 aagtattcat gagtttctcc agtaacttta tttttaaata caactgattc taatattcca
    61441 tcacctttga tttcttcaat tactgtatct aataaaaact ctatctttgg attagctttt
    61501 gctttttctt caacactctt agcacatcta aatccttgtc ttctatgtac aattgttact
    61561 tttctagcaa atttagttaa atacattgct tcttctaatg cactgtctcc tccacctaca
    61621 acaaatactt caaaatcttc aaagaaatct gcatcacaag ttgcacaata tgaaacccct
    61681 ttaccagtaa gttctttttc tccaggacaa ccaatctttc ttggtgttgc tcctgttcct
    61741 attattactg cttttgctct atattctgcc ttttcccctt ttaaaacttt tatatctcca
    61801 tctagctcag tatcaacaat gttatctctt agcatttctg ctccaaactc tttacactgt
    61861 tcaaccattc tagctattaa acttggtcct gttgcatttg gaactgaccc tggataattt
    61921 gcaatttcat gtgttattac aatttgtcca ccagtcttgt ctttttctag gattagagtt
    61981 tttaactttg ctcttgctcc ataaagacct gctgcaagcc cagctggtcc tgaaccaatt
    62041 attactaagt catacacatt ttccattttg acttatcctc cccaattaaa tttctcattt
    62101 tatttaatat taaattagat ttaatattta ggctgtttca ataacttttt aacaaatttt
    62161 tttattcata ctgcattctt tgtattgtat tataaaatat atcataatca attactctaa
    62221 aatcgtcttg gacattttct gtccattttg gagtcttttt attatcctga aatttacttg
    62281 cagtgatgat tgcttcttca attaaggtta aagagtttat atccaaacga tttcctttct
    62341 ttagtataac agtcctgtaa ggtgtttcat ttggttttac actcccatag agtggatgag
    62401 ataaaagttc ataaccttca tgtatcaaat ctctacttgc ttctaagatt ccaatataag
    62461 ttgtgtcttt aaacaaaact tctctgcctt gaacttcttc ttttactttt ggattattcg
    62521 taattattat cattttttta ctctctttca aacttttatt tattacttga taaataaaaa
    62581 acaggaaaaa caaatctccc aatttgtctt ccctgttaaa atcattttca gagccccgtc
    62641 caagtacagt cacctttacc tgagaatttc atctaatctt ttgggtaagt aagattaaac
    62701 ttgtcccttc ggtcgctttt tataaagcag tctcccatac tcttcatccg gattattatt
    62761 tttttcagaa aatttaaaat taagcaattt atcctttgtt ttctataaat agcttttttc
    62821 tatctctgtt gacttaagta tataaacatt ctataaaatt tgcaatagtt tttttatatg
    62881 tattttgtat acatttttac ttttttttcc aaatgttgat attgcatatt tcttgtattt
    62941 tctttttttt ttaagtacaa atctttttta gtttatttta tcaatttagt taaaaacaag
    63001 actattttca caacaaataa tcacttttta tatatttttt aatttttcac ttttatacaa
    63061 taaaattctc ataatttttt ataatttgct gtcatttatt tcatcaaacc aagatttaac
    63121 atcacctata atagactcaa tacagccatc atcaaatgga gaatacaaat ttcccatact
    63181 cacaatatct aaaaatgatt ttgttcctcc aactttacag atatttatat aatctttcca
    63241 tcctgcatct ctatcttgaa tcattttttt ccaaaattgt aacgcacaaa tttgagctaa
    63301 tacataatct atataataaa acggattttt aaatatatgt ccttgtttaa accaccaaca
    63361 acctctctct aaaaaagaat tatcaccata ttttctatga ggtaagtatt ttttctctaa
    63421 aaatctccag atttcttttc gttttgactt atctacatta ggatttttgt aaatatagtg
    63481 ttgaaattca tcaacaatta ctccataagg tataaattta attgaagatg ataagtgagt
    63541 gaatttgtac ttatctgtat cttctttaaa aaataaatcc atccacggcc atgttataaa
    63601 ttccatactc attgaatgga tttcacaact atcaagcgtt ggaaaattaa tttctggcat
    63661 atcaatccat ctagacatat acaactgaaa tgcatgtcct gcctcatgtg ttaacacatc
    63721 tatatcatca gcagtttgat taaaattaga aaaaatgaat ggtgacttat aatctggtat
    63781 ataagtacaa taccctccag ctccttttcc ttttttcgtt tctaaatcca taagttcatt
    63841 ttctaacata aaatcaaaaa attcatttgt ttcttttgac agttcagaat acatcctttt
    63901 accattttga attatgtatc ttgaatctcc ctttaaactt gcatttcctg ttaaaaattc
    63961 cacgccttca tctatgtaac ttaaatattc tagaccaatt cttttagctt gtttttcata
    64021 aagctcattg gccattggaa caacatattt taatacttgt tttctaacat tttttatcat
    64081 atcttctctg tagtcacttc tcatcattct aacgtaccca agctctacaa aatcttcaaa
    64141 acctagtttt ttagatatct tatctctaag tttaaccaat ttatcaaata tgtcatcaaa
    64201 tttagtttca ttttcttcaa agaaattata atatgctttg tttgctaatt ctcttgtttt
    64261 tctattctta gaatacatga atttccccat accagataaa tttcttatat tcccatcaaa
    64321 cattatctca gctgaagcta aaagtctagt atactctgaa cacaatttat tttcttcctg
    64381 caattcactt attatttctt tagaaaaact ttttaaagaa tactctacta ttgtgaaaaa
    64441 ttgttctcca aactctttta tcaaatcata tttaaatttt gaatttacaa tagcattata
    64501 aaaatgtgaa tttaattctt catacaaagg catatattca tcccaataat ttttttcttc
    64561 tttgtagaat ttattaaatg tgtctgtact atatcttatc gaagctatat ttgacaacgt
    64621 ttctatttta tttcttaata aattaatctt atgtatattt ttttgttgct ctttataatt
    64681 acgagaatta tttatatctt ctacacaact caaaaactct tttttcatac tattataatt
    64741 gggtctttca tacttaaact ctgaaaactt catttaaagc actcctttac aactttactt
    64801 tattgtattt ataaatatag atttacatgt actatgtaat acttttttat aaattattat
    64861 atatattaaa tattctttat attataatca tgttgaataa taacatatga ttaatggaat
    64921 tacaatattt tataacacaa aactaactaa tataacaaat taccatcagt ctatttattc
    64981 aaacttctaa ctatataata tacatttatt tatattaaca atgcaaacga taccattatt
    65041 tgtaattaag aattttggaa ttattgatta caaagttttt acattctcat ctcacataag
    65101 taatattttt atcaaaa
//
LOCUS       DAMKPG010000271        61047 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.011, whole genome shotgun sequence.
ACCESSION   DAMKPG010000271 DAMKPG010000000
VERSION     DAMKPG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.011"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            complement(116..808)
                     /locus_tag="PC621_003832"
     CDS             complement(116..808)
                     /locus_tag="PC621_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF4110890.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(834..2021)
                     /locus_tag="PC621_003833"
     CDS             complement(834..2021)
                     /locus_tag="PC621_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF4110891.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2357..4234
                     /locus_tag="PC621_003834"
     CDS             2357..4234
                     /locus_tag="PC621_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF4110892.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4546..5952
                     /locus_tag="PC621_003835"
     CDS             4546..5952
                     /locus_tag="PC621_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF4110893.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5980..6828
                     /locus_tag="PC621_003836"
     CDS             5980..6828
                     /locus_tag="PC621_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4110894.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7489..8181
                     /locus_tag="PC621_003837"
     CDS             7489..8181
                     /locus_tag="PC621_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110895.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8178..9113
                     /locus_tag="PC621_003838"
     CDS             8178..9113
                     /locus_tag="PC621_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110896.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            9261..10184
                     /locus_tag="PC621_003839"
     CDS             9261..10184
                     /locus_tag="PC621_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110897.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10168..10938
                     /locus_tag="PC621_003840"
     CDS             10168..10938
                     /locus_tag="PC621_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110898.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10948..11730
                     /locus_tag="PC621_003841"
     CDS             10948..11730
                     /locus_tag="PC621_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110899.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            12107..12643
                     /locus_tag="PC621_003842"
     CDS             12107..12643
                     /locus_tag="PC621_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF4110900.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12675..13064
                     /locus_tag="PC621_003843"
     CDS             12675..13064
                     /locus_tag="PC621_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF4110901.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            13101..13487
                     /locus_tag="PC621_003844"
     CDS             13101..13487
                     /locus_tag="PC621_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF4110902.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13614..15056
                     /locus_tag="PC621_003845"
     CDS             13614..15056
                     /locus_tag="PC621_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF4110903.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15353..16168
                     /locus_tag="PC621_003846"
     CDS             15353..16168
                     /locus_tag="PC621_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF4110904.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
     gene            16769..17953
                     /locus_tag="PC621_003847"
     CDS             16769..17953
                     /locus_tag="PC621_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110905.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(18330..19439)
                     /locus_tag="PC621_003848"
     CDS             complement(18330..19439)
                     /locus_tag="PC621_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF4110906.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            19902..21095
                     /gene="nifV"
                     /locus_tag="PC621_003849"
     CDS             19902..21095
                     /gene="nifV"
                     /locus_tag="PC621_003849"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF4110907.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            21120..23045
                     /locus_tag="PC621_003850"
     CDS             21120..23045
                     /locus_tag="PC621_003850"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF4110908.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            23067..24062
                     /locus_tag="PC621_003851"
     CDS             23067..24062
                     /locus_tag="PC621_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF4110909.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            24389..24862
                     /locus_tag="PC621_003852"
     CDS             24389..24862
                     /locus_tag="PC621_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF4110910.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            24896..25390
                     /locus_tag="PC621_003853"
     CDS             24896..25390
                     /locus_tag="PC621_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF4110911.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            25487..26008
                     /locus_tag="PC621_003854"
     CDS             25487..26008
                     /locus_tag="PC621_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF4110912.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            26197..26508
                     /locus_tag="PC621_003855"
     CDS             26197..26508
                     /locus_tag="PC621_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF4110913.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            26536..27222
                     /locus_tag="PC621_003856"
     CDS             26536..27222
                     /locus_tag="PC621_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF4110914.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            27454..28338
                     /locus_tag="PC621_003857"
     CDS             27454..28338
                     /locus_tag="PC621_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF4110915.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            28437..29612
                     /locus_tag="PC621_003858"
     CDS             28437..29612
                     /locus_tag="PC621_003858"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF4110916.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            29740..30594
                     /locus_tag="PC621_003859"
     CDS             29740..30594
                     /locus_tag="PC621_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110917.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKIKDDSSC
                     LYLDIYND"
     gene            30638..31873
                     /locus_tag="PC621_003860"
     CDS             30638..31873
                     /locus_tag="PC621_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF4110918.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            31974..32915
                     /locus_tag="PC621_003861"
     CDS             31974..32915
                     /locus_tag="PC621_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF4110919.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(33144..33497)
                     /locus_tag="PC621_003862"
     CDS             complement(33144..33497)
                     /locus_tag="PC621_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF4110920.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
     gene            33793..34704
                     /locus_tag="PC621_003863"
     CDS             33793..34704
                     /locus_tag="PC621_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF4110921.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            34964..35398
                     /locus_tag="PC621_003864"
     CDS             34964..35398
                     /locus_tag="PC621_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110922.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            35421..36956
                     /locus_tag="PC621_003865"
     CDS             35421..36956
                     /locus_tag="PC621_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110923.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            37040..38407
                     /locus_tag="PC621_003866"
     CDS             37040..38407
                     /locus_tag="PC621_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF4110924.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            38442..38666
                     /locus_tag="PC621_003867"
     CDS             38442..38666
                     /locus_tag="PC621_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF4110925.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(39116..39514)
                     /locus_tag="PC621_003868"
     CDS             complement(39116..39514)
                     /locus_tag="PC621_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF4110926.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(39517..39918)
                     /locus_tag="PC621_003869"
     CDS             complement(39517..39918)
                     /locus_tag="PC621_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF4110927.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            40277..41263
                     /locus_tag="PC621_003870"
     CDS             40277..41263
                     /locus_tag="PC621_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110928.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            41247..42158
                     /locus_tag="PC621_003871"
     CDS             41247..42158
                     /locus_tag="PC621_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110929.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            42271..43839
                     /locus_tag="PC621_003872"
     CDS             42271..43839
                     /locus_tag="PC621_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF4110930.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            43914..44939
                     /locus_tag="PC621_003873"
     CDS             43914..44939
                     /locus_tag="PC621_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110931.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            44932..45876
                     /locus_tag="PC621_003874"
     CDS             44932..45876
                     /locus_tag="PC621_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110932.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            46235..47893
                     /locus_tag="PC621_003875"
     CDS             46235..47893
                     /locus_tag="PC621_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF4110933.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
     gene            47874..48890
                     /locus_tag="PC621_003876"
     CDS             47874..48890
                     /locus_tag="PC621_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF4110934.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            49105..49734
                     /locus_tag="PC621_003877"
     CDS             49105..49734
                     /locus_tag="PC621_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110935.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            49869..50195
                     /locus_tag="PC621_003878"
     CDS             49869..50195
                     /locus_tag="PC621_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF4110936.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            50211..51500
                     /locus_tag="PC621_003879"
     CDS             50211..51500
                     /locus_tag="PC621_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF4110937.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            51524..51853
                     /locus_tag="PC621_003880"
     CDS             51524..51853
                     /locus_tag="PC621_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF4110938.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            52001..53329
                     /locus_tag="PC621_003881"
     CDS             52001..53329
                     /locus_tag="PC621_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF4110939.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            53646..54506
                     /locus_tag="PC621_003882"
     CDS             53646..54506
                     /locus_tag="PC621_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF4110940.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            54711..55691
                     /locus_tag="PC621_003883"
     CDS             54711..55691
                     /locus_tag="PC621_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF4110941.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            55894..56343
                     /locus_tag="PC621_003884"
     CDS             55894..56343
                     /locus_tag="PC621_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110942.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(56504..57433)
                     /locus_tag="PC621_003885"
     CDS             complement(56504..57433)
                     /locus_tag="PC621_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF4110943.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            57626..58438
                     /gene="modA"
                     /locus_tag="PC621_003886"
     CDS             57626..58438
                     /gene="modA"
                     /locus_tag="PC621_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110944.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            58587..59270
                     /gene="modB"
                     /locus_tag="PC621_003887"
     CDS             58587..59270
                     /gene="modB"
                     /locus_tag="PC621_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF4110945.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            59271..60347
                     /locus_tag="PC621_003888"
     CDS             59271..60347
                     /locus_tag="PC621_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF4110946.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            60353..60910
                     /locus_tag="PC621_003889"
     CDS             60353..60910
                     /locus_tag="PC621_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF4110947.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
BASE COUNT        23550 a         6686 c        10908 g        19903 t
ORIGIN      
        1 tgaaatgatg aaaatatatt tgatttcata caaaaaagag tgcctcataa ctaaaaagtt
       61 cactttgaga cactccattt ttatatgtta gttgcattta aggtaaaata gaagtttaat
      121 catgttgaaa taactgaatt acatcttcta aatgattgta cattgatgta taacctaagt
      181 aagagtcttt actatcaaat gccatcatta tcttgttatg gtggctaatc atatccttaa
      241 gagaatttct atatttaaaa ccgaagtatt ggttagaaga aactgtttta ggtattaact
      301 cataatattt tataaaaatt ggattatgag aagctaaaat tatctctttg tgaaattgta
      361 agtcagcatc ataacattct tctatcaaac catgagaaac cttatccaat tcctctattg
      421 actttaggta agtatatttt attttttgaa gttctttaga tgtagcattt aaaattgcta
      481 gtttagtagc ttcactttcg ataagttgtc ttgctcttaa agattctaca taaaattctt
      541 gtatgttatg atttttatag atttcaccat catcaaatag gtcaactttt ttaaatcctt
      601 caactatagg acttgagtaa ggcacagctc taagtattcc ttccttacag agtatattta
      661 tggcatttct tacaggtatt ctacttatgt taaataggtc agcaatcttt ctctcacttg
      721 gtaatttact attaatttta aaagttccat ttagaatgtt taatttaata taatcaataa
      781 ttttttcagt ggcatttaag cttttcataa aaactcctaa acatataatt tttttatgat
      841 tgtattatat catagttaag agttaaatct acttttttat aatatgtata agacaggata
      901 gaggccaaaa tagctataat agccaagata aagtatccat attgtattga agtatattcc
      961 gatattttac caactatcat tggaaataca gacattccta tagcataaat tgcttgaaaa
     1021 aaacccattg cagtagattt ctgatgtttt ggtacatttt tcatagactc gctagtaagg
     1081 taagaaagga gtatacctgt agatatacct ggtagcactt gtagtacaaa tattatatta
     1141 atatttttta ttgttggaac taaaaagcaa taggctgaaa ctaaaataaa aactaaagga
     1201 atccaaaatt ttggcccata cttagtacaa aatttagtag aagcaaagcc agaagaagca
     1261 acagcacaca ccatgtaaga tatagaagat agacctatga gggtagaact agaacctaag
     1321 tttttaagaa tttgagtagt aaatgacatt gttgtagaca tttgaatacc ctgttgaatt
     1381 aatgccataa aggaaaaaaa tacaagtctt ttatctttgc agatagataa atatttttta
     1441 attgatattt tttcaggtat tttttttgaa ttatgatttt ttatatttaa ggctagaatc
     1501 gttccaataa ctgaagacaa tacactcatg atacataagg ctgtcattcc taatttttca
     1561 taaaataaag tactcataac aaatccaatc aacattccta gattgtttac caatattatc
     1621 gtactcgtag ctttttgttg atgttctgta gaaaaaaagt tggtatagtg aaccatgtaa
     1681 gaaatccaag ttgatgatga tattccagat aaaatgttag ctataagaaa accattgcct
     1741 ccaggagaaa agactcttat aagagatgct aaaccagcaa agaaaattcc agacagtata
     1801 aatattttat gtttatttat agaatcagca aatactccaa caggaatcct taataaacat
     1861 tgtgagattc catatgcacc tattattata ccaatcatat ttgtagatac attacatgca
     1921 cttaaatatg gattttggaa aggtatatat acatattgtg aaaaccagaa aaatgtaaca
     1981 atagaaagta aaagtaattt ttgattagat atttttttca tattatcctc catatggtat
     2041 ttttataatg caaataatat accatttaga ttttttaatc aagtactttc tctattaaaa
     2101 aaaatttaaa tatataatta ttatttacaa aaaaattata ctttatagtt gaattaagaa
     2161 aacgcttatg atactataaa gttaacaaat ttgaatatat aacagtagac gtgttactgg
     2221 taatgcaggc aagatcgaat caatttttat agattatcta tagagtacta tgggtataat
     2281 ctatttattt ggttcgattt ttttgtttaa ttggccataa aacaaaaagc ctatagttga
     2341 aaatggagga ttttttatgg attataaaaa tatagctcaa gaaattctac taaatgtagg
     2401 aggaaaagag aatgtaaatg aagtgactca ttgtatgaca agacttagat ttaaagtcaa
     2461 aagcgcttct aaagtgaata aggataaatt atctaaaact gaaggagtaa taactgttgt
     2521 tgagagtatg ggtcaaatac aagttgtaat tggaaataaa gtaaaaacag tatatgatga
     2581 ggttataaaa attgtacctc aatcaaatga cgtaggtaaa aagaatgaaa gtcaagaaaa
     2641 gcaaagtata attaattcta tattatcagc agtagctggt atatttacac ctacaatacc
     2701 tgctatagca ggggttggta tgattaaagg tattttatca gttcttgcta tgtattatat
     2761 gaataaaaat ggagtagata taaaagaaac gcaaagttat attatcttaa atgccatggc
     2821 cgattctata ttctatttta tgcctattat actaggatat actgcggcta aagtatttaa
     2881 tgcaaataaa ataatatcta tggtattagg agctacactt tgttatccaa cttttactgc
     2941 tttgatggca ggtgaagaat ctgttagatt tttaggattg gcagtaacaa aagctacgta
     3001 tacatcatct gttattccaa taattattgc tatatgggct ctttcatacg ttgaaaaagt
     3061 attagaaaag tatatacctg aggtaattaa aataataatg gttccaactt tatcattggt
     3121 tgtaatgtta cctgcaacat tatttttatt tggaccaatc ggaatttata taggaaatgt
     3181 cataaacttt tcttataagt atatttatga attaagccca gcgttgtgtg gagcatttgt
     3241 aggtgggtta tggtgtgtgt tggttatatt tggtgctcat agagcattat taccaatagg
     3301 tataagtgat gttgctcaaa caggaagaca gaatttattg gcatttgcag gagctgcaaa
     3361 cttttcacaa gcaggggctg ctctaggagt attctttaaa actaaaaatc aaggactaaa
     3421 aacaatttct atgtctgcaa ctataacagc tttatttggt ataactgaac ctgcaatata
     3481 cggtgccaat cttagattaa aaaaacctat ggtatgtgcc gtaatatgtg gtgcatttgg
     3541 aggtgcgata atgggaatgg gtggagctta tggtaatgca tttgcaaacc aaggtgtttt
     3601 aactatacct gtatatgctg aagcaggtgc attaggtttc ttaagttatc taggtggttg
     3661 tgctatagca ttttttggtt cagctatttc aacttatcta gttggatttg aagatttgga
     3721 agagtcttct aataaggaaa attcatttat aaaagtagaa acgaaggatg gtgttattga
     3781 tataacttca cctgtagagg gcgaatgtat agaattgtca gaagtaaagg atgatgtatt
     3841 tgcttcaaaa gctatgggag aaggtatagc tgtgttacct acaaaaggag taataacagc
     3901 ccctactgat tgtgaagtag cttctttatt tccaacatta catgcaatag gattaaaatt
     3961 agataatgga gcagaaatgt taatacatgt tggaatcaat acagtagagt taaatggtaa
     4021 atactttaca aaacatgtta atcaaggaga tttagtgaaa aaaggtgata aacttatatc
     4081 gtttgatata gataaaataa aaaaggctgg ttatgatgta actacgccag ttatagtaaa
     4141 caatacattt gattttgggc aagtagtttc ttgtaaatct agttatgttt caaccaatga
     4201 taatataatt tcattagtat tagctggtaa ttaagttaat ttgactagag ataagtaaat
     4261 tgatttaaaa ggaaataagt aaatcaacaa ttaaatacat ctaaaagtaa gtttattgca
     4321 atgaattgtt aaagaaaata attaatatgg agtaaagcta ttgcaatgaa gtaacaaaga
     4381 aaataattaa tatagagtaa gtttattgta atgaattgtt aaagaaaata attaatatag
     4441 ggtaaatcta tctcaatgaa ataacaaaga aaagagttaa tatagagtga acttattgca
     4501 atgaatcaac aaaaaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt
     4561 ccaaaagact ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat
     4621 aaagacaata aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag
     4681 gcagatacta aagttgcatc agacttttac cataattatg aagaagatat tgaattaatg
     4741 aaagaattag ggatgaaaac atatcgtttt tcaatttcat gggcaaggat aataccagat
     4801 ggtgaaggag agataaacca gaaaggttta gacttttata ataagataat agacaaacta
     4861 atagagtgtg atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta
     4921 gtagagaaat ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct
     4981 aaaatatgct ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag
     5041 aatttaattg ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag
     5101 atagataaaa tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc
     5161 aatgcatgtc atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta
     5221 acatatcctc ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt
     5281 aaagtgtact atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag
     5341 tatcttgaaa aaagaaatac agttcctcac atggaagatg gggataaaga gatacttaaa
     5401 aaagctaaaa tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta
     5461 cctgaagatt cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat
     5521 ggactattta aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata
     5581 gacccatcag gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt
     5641 ataataacag aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat
     5701 gactatcgta tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat
     5761 ggtgtagaga tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa
     5821 ggttttaata agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat
     5881 ttaaaaagaa tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga
     5941 atagtaaaat aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta
     6001 aataattcgt tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa
     6061 ggcattggcc ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt
     6121 tttgttcttc atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat
     6181 gcagaatatt ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta
     6241 aaacttatga accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc
     6301 tttaaagaag gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca
     6361 aaagagtatg atataggaaa atatgctatt actgttttta aggaggttct tggtttagac
     6421 ctaccagaag aagagattgg taatatagca ggccatttta taaatgcaca acaagataat
     6481 ccttatagtg atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt
     6541 cattatcact tttctattgt atataataaa gagtcatttt attataggag atttgtaatg
     6601 catctaaagg ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat
     6661 tttatatatg agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta
     6721 ggagcttata tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg
     6781 atgattcata ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata
     6841 tgctagtaat attataaaaa attaaaaatt aactacactt taaatatgaa caattgcatt
     6901 acttaagggg gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt
     6961 ttaaaccttt atatcattac aactttaatg tttatacgcc tgtactttag atgtatatct
     7021 actttttaca tttctcaaca tgcaaaccta ccattttttg cagtatgtta attgaaataa
     7081 accattgagt taaccctaaa tttattctgg acttatagga atagttccaa agatacttta
     7141 aaatattttc tggaataatt gcttatttca catatgtttt attgtgatgt tttgatttaa
     7201 taaattcttc aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac
     7261 ttataatacg cttgattttt tcacaatgat gaaatatact aatgggtgtt ataggtataa
     7321 gaatcgaatt acacttgaag aagtaagtga taaatgagat ataaatcctc atatgataaa
     7381 ctactatttc attgaggaac atattctaag tgatgtcaag atactacaat atgactaaat
     7441 tcctagtgat aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt
     7501 ttaattatag atgatgataa agatctttgt attttgttaa aaaagagtat ttcaattgaa
     7561 cagattgaag caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa
     7621 gattatcagt tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg
     7681 gaacaaatac gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat
     7741 tacagcaaag tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa
     7801 attacagaat ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca
     7861 aaaaatgagg aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat
     7921 acagtgacta ccaatggcga acctatgttg cttccaccca aggaatttgg tgtgcttctt
     7981 tactgtgcaa ggcatcaggg taagattttg acaaaacagc agatttatga agctgtgtgg
     8041 ggtgagccat atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaagaaaa
     8101 aaaattgaac tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat
     8161 caattcaaca cggaggtatg aaaatggatg aacaaacgat aattcttttc ttattcttgc
     8221 tgtcgattgg tctacttttt ctttccatat ttgctttttc caagcttggg catattcaca
     8281 aacgcctaaa agatatagag gagatactgg ctgatgtaca aattggaaac gaaaatcgca
     8341 aaatactaat taagccatgt gatgttatgg ctccattggt ttatcagctt aatgagattg
     8401 tctacgatta tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac
     8461 ttatgacaag tctatctcat gatgttcgta caccattaac aactctgatt ggttatctgg
     8521 atgctgtaca cagtggtata gttatcggac aagaacggga ggaatacctt gaaatcgcta
     8581 ggcgtagagc ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga
     8641 actctgatga atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata
     8701 ttttgaaaga ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc
     8761 cagaaaatcg actaatggta aatctcgacc cagatggata ttcccgtgtg gttaataatc
     8821 tggttcaaaa tgttcttgct cacagtaagg ctcggcaaat caagattacc atgagtgagg
     8881 attccagaat ggtattactc cgtgttgatg atgatggtgt tgggattgcc agagaaaatt
     8941 taccacatat ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg
     9001 gtcttggtct atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag
     9061 aaagtgagat tggaaagtat accgtattta cagtgagctt accattaata taaaattgtt
     9121 tttcccccat gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt
     9181 taatacaagg ttgttgcaag gttaatgtaa gattgaggtg gtatgatagc tttaataaac
     9241 aaaggaggat tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc
     9301 aatatggtgc tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt
     9361 acggattgct aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc
     9421 taacaaaacc aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga
     9481 agaagcttct tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga
     9541 caggaacaga aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag
     9601 tagaatcagc actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat
     9661 actctcttgg tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac
     9721 tgttgatttt ggacgagcct atcaatggac ttgatcctat tggtattgca gaagtaagaa
     9781 agtttattcg tgatctctgc acagaacgag gtaaaactat tttgatttcc agtcacattc
     9841 tttctgaaat tgccttgttg gcagatgaca ttggtattat tgaccatggt attttgttgg
     9901 aggaagaaag tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat
     9961 ctgatgcagc acatgcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag
    10021 tagcagataa ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc
    10081 gtgctttcat tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg
    10141 aagattactt caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa
    10201 ttttcaaaac tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt
    10261 ccaattcccg caacaatttt gattgcaaag gacaacttgc cctttgagca attgttcaaa
    10321 ttagttgtca attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc
    10381 caactatttt ttatagaaag agataacgat gtattaaaaa atcttgcaac agtgccagtg
    10441 ccaaaaggaa aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac
    10501 tccgttgcag ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg
    10561 gttactgtaa aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt
    10621 cctgttgtgg tactcattgt atatttcaat cgcagctata ttttttctat tatactttct
    10681 tttgtatatg ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat
    10741 aacccactat taagcgtatt acctgctcct gttataatgc gttggtggat ggcattttgg
    10801 ggagatccca ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct
    10861 tgtgtaggta ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac
    10921 aaaaagcagg agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa
    10981 ataaaaagat ataatattct atggattggt gttgttgctg ttctgttttc agcactattg
    11041 gcagtttttc aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca
    11101 aacggagtta tctggaataa tttcagtctt agttttccat ttgggattac acttattgga
    11161 ggatatctta ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct
    11221 atttcattac gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tgctgtattg
    11281 ttaagtcttt tcagcttctt atgcacattt attttggggt tgttattttg caatatcaat
    11341 atatctgtta cactcattgt aaaatcctta gttcagattt tgtctgttaa tctgtattgt
    11401 tatgttgctg ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg
    11461 ggtgtaggga ttgcatttgt atacgggttt tgtggtgtgt ttgttgctgg aagaaatctg
    11521 acagactttt atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt
    11581 actgtttatc agccacttat cggtgctatg actttgattg taattctaat cttgacaact
    11641 attctacttg tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg
    11701 ataaagcata aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc
    11761 tagacagtga aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc
    11821 tacagttttg atttttcata actgtagctt ctttttgtat ttattaaatc acggctatca
    11881 taatacttgc aataggtata gcgacaagag agagtactgt catgtgtgtt ttatatatta
    11941 cattaagtat attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa
    12001 ataaaaaaaa taatattata tatagttgac aaaaattggc aaatgatata atatataaat
    12061 atagaaatag taatcattac tattattaaa aagggaggaa ttaattatga acttaaaagg
    12121 aactaaaaca gaaaagaatt taatggcagg atttgcagga gagtcagaag caagaaataa
    12181 atatacatac tacgcttcaa aagcaagaaa agaaggttat aatcaaatag ctgctatatt
    12241 tgaagaaact gctaacaatg aaaaggaaca tgctaaatta tggtttaaac ttttacatga
    12301 tggtatgcct tcaacagagg aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg
    12361 gactgatatg tatgcaaaat ttgctaaaga agctagagaa gaaggatttg acaagatagc
    12421 ttatttattt gaagctgttg gaaaaataga gaaagagcat gaagaaagat acttaaagct
    12481 tttagaaaac ttaaatgaag gaaaaatatt taaaagagat gaagaagttg tttggcaatg
    12541 tcaaaactgt ggtcatgtgt atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga
    12601 ccatccaaaa gcgtatttca atataaaagc tgaaaattat taaaaattat cattattaaa
    12661 aaggaatgtt aactatgaaa ttttctaaac aacgagaact gattttaaat gaaatattaa
    12721 ataatccggt tcatcctact gcggattacc tatatgaaaa cttaaaaaaa gataatccaa
    12781 atttaagctt aggaactgtt tatagaaatt tagctcagct aacggaacat ggctttataa
    12841 gaaaggttag tattccaggg tatccagata gatttgatgg tcgaatagat aatcactatc
    12901 atatcatatg tgaagtatgt ggagaggtat acgatttaga atccgaagtt cttaataatt
    12961 tacaagaatt aatatctgag gagacagaca taaaaataac atcttataac ataagtttta
    13021 aagggatttg taataattgt aaaaggtgta gccaagtagg ttagttttca aattctaaaa
    13081 ataattaatg aggtgagttt atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg
    13141 gaaaccttgt aggaatgata caaagtggtg gtgtacctat tttctgttgt ggaaaaccta
    13201 tgaaggaact tgttcctaat acaacagatg cagcagttga aaaacatgta cctgtaattg
    13261 aagtggatgg aaataatgtt actgttaaag taagtagcac aactcatcca atgactaaag
    13321 aacatcatat agcatgggtg tatcttatga ctgaacaagg tagtcaacgc aaatgtctag
    13381 cagtagatgg agaacctgta gttaaatttg ctttgaatga tgatgataaa gtgatttctg
    13441 cctatgctta ctgtaattta catggattat ggaaggctga actgtaatat ttaaataaat
    13501 tgagtcaaag aggaggctat aaatgtattt tcatacaaat agcctcctat aaatttatat
    13561 aaattcaagt tttaattttg tttaatgtca atataatata aggagaaaat aatatgtcaa
    13621 tttataaatg tagtgtttgt ggatatattt atgatgaatc taagaatgat aaaacatggg
    13681 atgaattaag tgaagattgg gaatgccctg tatgtacaaa aggtcgtagt tattttggaa
    13741 agataagcac tgtttactat gaagaagatg aaaagatagc agaagacata gttgaaaatg
    13801 aatctaaatt aaatactgaa aaagagggag atttaaatta cttaagtaca tatttaagaa
    13861 gagatgatga agttgagaaa catatggata taattcacga aatggctgtt acaggaaaat
    13921 ctataataga acctatgaga actaaactac cagtaatttc ttgggatgat attttaatta
    13981 tgggtgctca attaaatcca ttacctttaa atgagcatga tgaagtaaat acaacaacaa
    14041 taataggaaa aaaagcaaag aaacctatga ttatagaaaa tccagtttat atttcccaca
    14101 tgtcatttgg agctttgtct aaagaactaa aaatagcttt agcaaagggt gcagcccaaa
    14161 ataagacagc tatgtgtagt ggtgaaggag gtatattacc agaagagaag gaagcttcat
    14221 acaagtatat ttttgagtat gtaccaaata aatatagtgt aactgaagaa aatcttaaaa
    14281 attctgatgc tatagagata aaaattggtc aaggaacaaa acctggtatg ggaggtcatc
    14341 ttcctggcga aaaagtaact gaagaaatag caaaagtaag aaataagcct gtaggtcaag
    14401 atgtaataag tccatcttgt tttgaggaaa tacaatccaa agaggattta aagaaactta
    14461 tagatgaact tagagaagtt tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc
    14521 atattgaaaa agatatggaa tttatagctt atgctaaacc tgattttgta actattgatg
    14581 gtcgtggtgg agctactgga gcaagtccaa agttgcttaa agatgcaact tcaattccaa
    14641 caatatttgc tctttatcgt gctagaaaat acatagatac acatggatta gatatagatt
    14701 tggttataac aggtgggctt cgtatatcta ctgattttgc taaggcaatt gctatggggg
    14761 ctgatgcagt tgctattgca agttctgctc ttatggcagc ggcgtgtcaa caatatcgta
    14821 tatgtggttc tggtaagtgt cctgtaggag tagctactca ggatgaggaa ttgagaaaaa
    14881 gattacatat agaaaatagt gctaatagag tggcaaactt tttaaatgta agtttagaag
    14941 aacttaagac ttttgctaga atttctggtc ataaagatat acatgattta agtgttgatg
    15001 atttatatac tgttaactct gaaatatcaa attatacaaa catacagcat gtatagattg
    15061 aagtttgaaa ctattagatt ttagatagct ataaaagtaa atgataaata tattttataa
    15121 aagagagtgt cttaaaatga actttttagt tcataagtca ctcttttttg tatgaagcca
    15181 aatatattta tcattttagc aatgaaaaag aatcttatct ctttattttg agatagactc
    15241 cttctattta tgaaaattaa agatattaaa ttgtaaaata ttctgtgatg tcgtactatt
    15301 aaactaaaga ttctgtactt taaagtaaat aaaaatttaa ggagtgctta ttatggaaag
    15361 attgaagata caagttttag ttgacaataa tacttatatt gatagatact ttgttggaga
    15421 accagcagta agctattata ttgagattga tggaaatagg attttgtttg attcgggata
    15481 ttcagatgta ttcatatcaa acgctgaaaa attaaatata gatttaggaa atttaactca
    15541 tgttgttttt tctcatggac ataatgacca tacaagaggt atccagtttt tagaaaatag
    15601 gtatgattta tctacagttg aacttatttc acatcctaac tgttttatac ctagaaaaca
    15661 tggagagaaa agcataggag ctccattttc tgctgaggaa attaaaaata tttttatgta
    15721 taatcctaag gataagccat ttaatctaag taagaattgt gtgtttttag gtgaaatacc
    15781 ttctataaat aattttgaaa aaagagctaa aattggtaaa tgtaaaatag gagatttgtg
    15841 ggaagatgat tatgttttag atgattctgc tatagtttgt aaaactgata aaggtatttt
    15901 tattgttaca ggatgttctc atagtggaat ttgtaatata gtagagtatg ctaaaagggt
    15961 atgtggagat gatagagtta ttggtatact aggtggtttt catctttttg aattgaataa
    16021 taggttagat agtacaatac aatatttgga taaagaagaa ataagaatgt tgtacccttg
    16081 tcactgtgtt tcttttaagg taaaagctaa gatgaatgaa atattgaata taaatgaagt
    16141 tggagtaggg ctgaccataa gtatatagta tttgaaagta tataagattt aagttgaagg
    16201 ttaaaaagat attttctata taaagtatct tttttgttac gtttatttta agagaaatca
    16261 atatactttt atatgaaata ttgtaaaaaa atttattttt aatgagaata tattttcatt
    16321 aataaacaat tttattatgt attaataatg ctagaataaa tataaataaa tcaaagttac
    16381 ttacattaaa gtatattttt tcacaaaata tattgattta ttcttttaaa gtttgataat
    16441 tccaatgaat gtaataaaag aagggatttt atactataca attattatta taaaattata
    16501 aagaaaaaat taaataataa taaagaaaaa atgtataata tttaacatta aaaaagatat
    16561 ttcataaaat taaaaaacaa agtgaatttg aatactattt aaaaaataaa aaaatatata
    16621 aaaaatattt aatacaaagg tagaaataag ttgaaaatgt aaagtcattg gaaaaatata
    16681 gttgaattta atgaacaaaa atgttattgt tttatcaatg aagtgttaaa ataaaaactt
    16741 tcaatatcat tttagggagg atgatttcat gcaaaaaagt gtaaagaaag caaaagtaac
    16801 gggatctatg ttagccattt ttggtgttgc aagcgttctt tttagttcac atgcaggagg
    16861 aggatttgct acagggaatc aagaaactca gtactatgtg cagtatggat ggacagcacc
    16921 acttatggca attttagcaa tgattatact tactgctacc atgagagaag ttatcataat
    16981 gtataataat aacaattgta ggaattataa ggatttattt tgtgagcttt ggagaccata
    17041 tccaaaactt gaaataatat gggagatata ttactatcta atggttttaa tagctgtaag
    17101 tgctgttata gcaggtgcag cagcagtatt tcaaagtata ggagtaaatt attttgtagc
    17161 tgtatttatt attggagttg tacttcttgt ctttactata tttggtgcta tgcttgtatc
    17221 taaagcagct acagcaatga caatagctat tctagtatgt actcttacta tttttattgt
    17281 tggtataaaa gctaaagtac cagaaataac tgaaatatta tcaaatagaa cgagtttcac
    17341 acctggctat atcaagccaa tacttaatac ttttatttat gcaggttttc aatcggttgt
    17401 tatacctaca cttgcaggat gttcaagacc acttcttaag aactcaaaag aagctactaa
    17461 ggcaatgata atttcatttg ttatgaatgc aattgcatta ggacttgctg taactatgct
    17521 aatgggatgg tatagagaaa ttattgctgc tgggcaaaca acactcccta ctctttatgt
    17581 tgcaggtcaa tctggaaatc atactatata cataatttat aatgttgcat tattcctttg
    17641 tttaatgtct acaggagtaa ctactatatt tggtcttgta aatagatttg aagaccataa
    17701 agcactttct ttcctttctt caagaatgaa aagaagggta tttacagctt gtgcaataat
    17761 ggtagtgtca atgcttatat cacttacagg attaagtaat attgttaaat atggatatgg
    17821 atattgtggt tatcttggtc tattcactat agtaattcca ttccttacac taggacatta
    17881 taagaataag aagtttgcaa aggaaaatcc agaagctaaa tggccagcgg aattaaatga
    17941 aaatgtaaat tagatgtagt agcaaaaaat attgctaaag agaatactat aaataagaat
    18001 actatagata atatatctgc atgtttatta agattatttg caaaatctaa tattgaacaa
    18061 ctataaacaa gcaatataaa actaagaata aaagataaaa agagggtgtc tcaaaatgga
    18121 cttttagttt ataaggcact cttttttata tgaagtcaaa tatattttca ttattttgac
    18181 aataaaagag actaatccta ctttggatag cctctttttt ctttaatata aaatagtata
    18241 acagcaaagt atagattgat acatgttttc tgtagctttc tatatattac agtgtttgtg
    18301 tggcattggt attttaagca atctataaat tataattcta taattcttct actaatttta
    18361 gattctatag ccgtctctat ttcacttaac attcgttcat ttttagggtc aataaaagca
    18421 catgtatagc cttcttcacc agctctacca gttctaccaa tacggtggat gtagtcttct
    18481 ggtgtttctg gtaaatcgta attatatata tgacttactc cagatatatc aaggccacga
    18541 gaagctacat ctgtagctat taagtattgt atatctaaat ttctaaatga tttcattatt
    18601 ctttctcgtt ttgattgagc aatatcacta tgaagttttt gacaattata tcctcttata
    18661 gcaagagctt cttcaagatt gtcaaccctt ctcttagtcc tacaaaatat tattgccata
    18721 aaaggattat cttcatctag taccttacat aaggcatcta atttttttct atctgtagtt
    18781 tctactactt cttgttttat attattaaga gtgatttctt ctttttgaat agaaactaca
    18841 acaggatttt ttgtgtatct ataagctaat ttttttactt gagaatttat agttgcagaa
    18901 aagcatagtg tttgcttttt gtttgaagtc tcttttaaaa tagcttccac ttcgttttta
    18961 aatcccataa gcaacatttg gtctgcttca tctaaaacaa aagtctttaa cttactaagg
    19021 tctattgttt ttctatttag atggtctaaa agcctacctg gtgtagctat aatcatatga
    19081 atatttcctt ttagtttctt tatttgagaa ccaatatctt ttcctccata tgctgcaaga
    19141 atacttacat ctttagattc tttaagtttc atagcttctt ctgttatttg aatagctagt
    19201 tctcttgtag gagaaagtat aagtacttgt atatcattta tatctaatga tatattttca
    19261 aatatcggaa gtaaaaaagc tagtgttttt cctgttccag tttgagcttc tgctattaca
    19321 tcttttcctt cttttattag tttaatactt tctttttgta taggtgtagg tgttgttatt
    19381 cctgattttt ttagaatatt tattgtattt tcactaatcc ctaagctttt gaaatccatt
    19441 gttatcttac ctttcatgaa aaattttctt ttttattaac caattaataa tatcatattt
    19501 gtaagtattt ttagtaaatt ttttaattat tatttatata gcttaaaaat atattaagat
    19561 actttaaaat ggaatgtaac ttaacttaaa ttttagtagg aattttatag gtttattttt
    19621 aaaggttttt tgaaaataaa tctatttaaa aattaaatta aaatgcaaga aaaacataat
    19681 aactttcatt tttaagaagt tttcacaaaa aagaaaaaat atagtgcaat aaatgataca
    19741 aagtaatata atagattaca agatatgaaa agctaaaaaa ataaattgca aatgaaatta
    19801 aaatgaataa aaaataaaaa aataatcata agttagaggt atcaatgaaa aataagactt
    19861 aatgttttta aaattaaaag tatactaagg ggggcattaa tatgtgcgtt atatcaaaag
    19921 atagagcaaa ggaaataaaa atagtagata ctactttaag agatggagag caaacagctg
    19981 gggttgtatt tgcaaataga gaaaaaataa tgattgctga aatgttaagt gacctaggtg
    20041 ttgaccaaat tgaagttggt atacctacta tgggtggaga tgaaaaaaat gtaataaagc
    20101 acatatgtag tagaaattta aaatcagata taatggcttg gaatagagca gtaataaaag
    20161 atgttgaaga atctatttct tgtggtgttg atgcagtagc tatatcaata tcagtatctg
    20221 acattcatat agaaaataaa ttgagaactt ctagaggctg ggttttagaa aatatggcta
    20281 aaacagttga atttgcaaag aaaaatggat tatatgtttc agtaaatgga gaagatgctt
    20341 caagagcaga tatagacttc ttaactgaat ttataaatgt tggaaaacaa gcaggggcag
    20401 atagatttag atactgtgat acagtaggag ttatgaatcc tttttctata aagaatgcaa
    20461 tagaaactct ttatgaaaga actaattttg atatagaaat gcatacacat aatgactttg
    20521 ggatggcaac tgcaaatgca cttgcaggaa tagcagcagg agcaaattat gttggtgtta
    20581 cagtaaatgg attaggtgaa agagcaggaa atgcagcatt agaagaagtt ttaatggcac
    20641 ttaagtgtgt ttataaatgt gatttaaata atatagatac tagaaaattt agaggaatat
    20701 gtgagtatgt agcacaggca tcaggaagaa tacttcctac atggaaacca gtagttgggg
    20761 ataatatgtt tatacatgaa tctggtatac atgcagatgg agctttaaaa gacccgcata
    20821 actatgaacc atttgaccca agtgaagtaa atcttgaaag aaaaatagta ataggtaaac
    20881 attcaggaag agctgcagtt ataaataagt tgagtgaata tgaaatgtat atttctccag
    20941 aaaacgctac caagctatta aatgctataa gagctacatc tataagatta aaaagaagtt
    21001 taatggataa agaaattctt caattatatt gtgatatact agcacatgag aaaggcacaa
    21061 cagaagaaga agcagttaga ggttcatata tttaagatat aattgtttag gaggaaaata
    21121 tgggagataa tatagtttat aaaataatta aaaaacatat agtagatgga gaagctgttg
    21181 ctggaagttc aattggtata aaaatagacc aaactttaac acaagattct actggaacaa
    21241 tgacatatct tcaactagaa gctatgggaa tagataaagt aaagactaaa agaagtgtgg
    21301 catttgtaga ccataatatg ttacaacaag gatttgaaaa tgcagacgac cataagtata
    21361 tacaaacagt ggcagataaa tatggagttt atttctctaa gccaggaaat ggtatttgtc
    21421 atcaagtatt tttagaaaga ttttcaacac caggagatac acttttaggt tctgatagcc
    21481 acacacctac tgctggaggt gtgggcatga tggctattgg agctggtgga ttagatgttg
    21541 ctttagccat ggcaggagga gcatactata taaaagcacc aaaagtctgt aaggtgaatc
    21601 tagttggaaa attaaataat atggtatcat caaaagatat aatattagaa gtgttaagaa
    21661 aacaaactgt aaaaggtgga gtaggtaaag tttatgaata tggtggagaa ggtgtcaaaa
    21721 gcctatctgt tccacaaaga gctacaataa caaatatggg ggctgaactt ggagcaacta
    21781 cttcaatctt cccaagtgat gaaaaaacac tagaattttt taagagtcaa ggaagagaag
    21841 acgcatggat agagttaaaa ccagatgcag atgcagttta tgatgaagag attactataa
    21901 acttggatga attaaaacca ttagctgcaa aacctcatag cccagacaat gtagatgaag
    21961 ttgaaaatat aggcaagatt aaaatagacc aagtggcaat aggaagttgt acaaattctt
    22021 cttatgaaga cttaatgaaa gtagcacaaa tcctgaaagg aaataaagta cataaggatg
    22081 tgagtttggt aatagcacca ggttcaagac aagttatgga gatgattgct agaaatgggg
    22141 ctttggcaga tataataagt gctggagcta gaatactaga aaattcttgt ggtccatgta
    22201 ttggtatggg tcaatctcca ggtactgact ctgtgtcttt aagaactttc aatagaaatt
    22261 tctatggtag aagtgggact ttgtcagcac aagtatattt agtaagtcca gaagtggcag
    22321 cagtaagtgc aatcaaggga gtgcttacag acccaagaga atttgatatt aaatttacaa
    22381 atttagatgt aaatgaattt ttgatagatg atagcatgat aattaaacct gctgatgtag
    22441 gaagtaatgt agaagtagtt agaggaccta atataaaacc tttccctctt aatacagagc
    22501 taagtcagtc tataggtgga aaagttatat taaagacaga agataatata actactgacc
    22561 atataatgcc atcaaatgct aagttacttc cttttagaag taatataccg tatcttgcaa
    22621 actattgttt caatactgta gatacagaat ttcctcagag agcaaaagac aataatggtg
    22681 gatttatagt tggtggagat aattatggtc aaggttcaag tagagaacat gcagctttag
    22741 caccattata tctaggtgtt aaaggggtaa tagttaagag ctttgcaaga atccacaagg
    22801 ctaacttgat aaatagtggt ataattccaa tggagttttg tgatgagaaa gattatgaaa
    22861 atatatctct tttagataat ctggagatac caaatatttt agacaatcta ggtagtggaa
    22921 ttttagaagt taagaataca actaaaggaa cttcttttaa ggtcaaagta gaattgtcag
    22981 caaaagaagt tgacgtatta aaagctggtg gaaaattaaa ctacactaaa aatcaagcta
    23041 attaatatga aaagagggaa tattgaatgt ataaagtgac acttatacca ggagatggaa
    23101 taggaccaga agttgcgaaa gctatgaaaa aggtagtaga agctactgga gcagaaatag
    23161 aatgggaaga agttaatgca ggagaggcag ttattgaaga gtatggtact ccacttcctg
    23221 aatatattat agatagtata aaaaagaaca aaatagctat aaaaggccca attactactc
    23281 ctgtaggtaa aggatttagg agtgtaaatg taactttaag acaggcatta gatttgtatg
    23341 tcaatttaag acctattaaa agttttaagg ggataaaatc aagatatgaa gatgttgatt
    23401 tagtggttgt aagagaaaat acagaagatt tatatgctgg aatagaacat aaaataggtg
    23461 attatgcagc tgaaagtata aagataataa caagaagtgc aagtgaaaga atcgttgatt
    23521 ttgcatgtaa ctatgttaaa gataataaga gaaaaaaagt tacagctata cataaggcaa
    23581 atataatgaa aatgtctgat ggattgtttt tagatgtgtt tagagaagtt gcatcaaaac
    23641 atggtgtaga atatgatgat ttgattgtag atgcagcagc tatgaactta gttttaaatc
    23701 cagaaaatta tgatgtaatg gtaatgccaa atctttatgg agatatacta tcagatttag
    23761 gtgctgggct tgttggaggt ttaggtataa ttccaagcgc aaatattggt aaagattgtg
    23821 cgatatttga agctgttcat ggaagtgctc cacaaatagc aggtcaaaat aaggctaatc
    23881 caacagcact tattcaatca tcagtaatga tgcttagata cttaggtgaa tatgaaaatg
    23941 cacaaaaaat agaaacagct ttagaaaaag tatttcttga aggaagtaaa ttgactgtag
    24001 atttaggtgg ctcagcttct actacagagt ttgcggatga ggtgtgtaag tatattgtat
    24061 agattttatt tagggaaaaa gtgaaatata gattgaagga aagaggcttt ctcaaaatag
    24121 agataagcct ttttttattg gaatgataaa atatatttga ttttatacaa aagagagttc
    24181 ctcataaacg aaaaagttaa ttttgggata tccattttaa ttttaaaatt aattgattaa
    24241 tacgcaaaga aagattaaac aaatttatta tttttaattg aactttcaac caaaatatgg
    24301 taaaatttaa aaaagttgaa ctttcaacta aaaataaata tattgattat gtgaaattct
    24361 ataaaacaga aactatttga ctttagtcat ggtaggttca gtggaggtgt gtttgattaa
    24421 gactttagat agtaatatat taagagaagt aggtacttta tctagagcag taaattcaat
    24481 aaatgatatt aagtacaagg aattaaagtt acaaaaagga caatttacat ttttgacaag
    24541 aatctgtgaa aatcctggta taaatcttgt ggaattatca aatatgttaa aggtagacaa
    24601 ggctacaaca accaaggcta tacaaaaact aataaaggca ggatatgtag ataaaaagca
    24661 ggatgagttt gataaaagag gatataattt aactccaact gataaatctt tggaagttta
    24721 tgagttgata attgaagaag agaatagaag tatagagata tgttttgata attttacaga
    24781 tgaagaaaaa caagtggtaa ctaagttatt agaaaaaatg agcaaaaacg tagaaaatga
    24841 atggtttaaa gtaaagagat agtaaataaa tacattaact taaggagata tttttatgat
    24901 tagaaaagcg aatatgaatg atttagaaag cattatgaaa attataaaat ctacagtgga
    24961 agaaatgaag acttataata atacacaatg ggatgaaaac tatccattag aaaaagactt
    25021 tgtttcagat attaagaaac aagatttata tatttatgaa gtggatggag aagtagcagg
    25081 atttatatgt cttaattatg aagagccaga agaatattta ggcttaaatt ggtcatctaa
    25141 taaaaaagca atggtaatac atagaatggc tgtaaatcca aactttagaa agacaggaat
    25201 agcatctaag ttagttgatt ttgctgaaaa attagctgta gaaaacaatg tatcatactt
    25261 aaagagcgat acatattcaa taaactcaaa aatgaattta ttacttacaa agtgtggttt
    25321 tataaaaatc ggagaaatga gttttttagg aaaggagaaa tctttctact gttatgataa
    25381 aatattataa agagcattta taatacaagg ggtaaataat agattttcag cattagtaag
    25441 ggcataatat aacccagtat atgctattat gaaagggatg atttatatga ttggatttct
    25501 aaagaaaaga agaagtatta gaaaatacaa ggatgttgaa gtagaaaaag aaaaattaga
    25561 taaaatatta aaagctgctc ttttagctcc atcatcaaag gggcttagaa catgggaatt
    25621 tatagtggta gatgataaag aaaagctaat taatttatct caatgtagaa ctaagggtgg
    25681 tggatttttc ttaaaaaatg cacctttagc tattgtaatt attgcagata aagaaaaaaa
    25741 tgatgtttgg atagaggatg caagtatagc tgcttcatac atacaacttc aagctcatga
    25801 actaggttta ggttcttgtt ggatacaagt aagaaataga atgtatgatg ataatataga
    25861 agctgataag tacattagag aagaattaaa agtacctagc aagtacagtg tagaatgtat
    25921 aatttctata ggatattctg atgaagaaaa gaaagcatac aatgactctg atttagacta
    25981 taaaaaggtt cattttaaca acttttagta tataatatgt tgtattaaga cattatattt
    26041 ttttctattg ttaatatata cttaaaattg taaaaatata tctgaatttt actaacttat
    26101 ctaggggtta tgtacaagga ttttaaagat aaatatagaa attaatgttt ataatcgata
    26161 cataacttga gtaacaaaat ttaaggaggt taaagtatgg gtatatttaa aacaaaaatg
    26221 gatgaggact ggaaagtaaa ctacattaaa gagtttaatg aaatgagaga ttcttatgag
    26281 agtaaacttc aaaaaaaaca gtttgaggta gatagtttga aatcagaatt ggatagacta
    26341 agaagctata aaaatagcct taagccaaaa gaaaaacaaa ttactgatga tgatataaat
    26401 aatataaaga gtttaagaag agatggctta agctataaag aaatttcaaa tcaaacaagt
    26461 tggagcaaag caactgtaag tagggtatta aatggacttt acgattagca tatacatggg
    26521 gtgatttaaa ttgggatgaa gtatagttac aaaaaatgca taatagattc tattttattg
    26581 atgtttataa ttcaaatatt aaggatgatt ttgaattatg tacttctaag tcaatttgaa
    26641 tttactcttg aaaattttaa tattataaac ttaatttctt ttacattagt tggtttatcg
    26701 ttaattttat ttttaaaaga taattctttg tataacaagg ttagaaatag aaaaatcaca
    26761 gaagcttttg aagaaaataa aaacaatata cttattgaaa agtgtaagct tatacttttt
    26821 gttgtggttt tatctttagc aataattgta acttattgta ctaagggata tgtacttttt
    26881 aatgtaacta tgatgacttt gtctgtattg atagttccaa tatttgaaga gttatttttt
    26941 agagaatata tatggaacta tctaagtaat tttataaagt caaagagtaa aattatctgt
    27001 ataacatcta tactatctgg aatttataat ataggataca tagatgttat aagaaattat
    27061 gtaatattgt ataacaattc aagctacacc tttgaagtta ttatttcaaa aattatgata
    27121 ggtactgttt ttggtattgt acttggatta gttaagtata ggtttagaga tgtagggttt
    27181 tgtatattat tgaggagctt atttgctata tttattaggt agatagtata atcggtttat
    27241 aaggttgttt taaatttaat tttaaatcaa ccttatttta tttgtgtttt ataaaacact
    27301 tatatattaa taactaataa atttaataaa gattatttga atatttttaa aatatataaa
    27361 tatgtattga catataaata ttatgaatta caatttatat aaactaataa aaattaaata
    27421 atattaatca acttattgaa tggaggaata aaaatgaaat ttgtgacttt ttgtagtagc
    27481 aatgaagaaa aaataggtgt atttaattca gaaacaaata gtatatatga aattaatagt
    27541 ttaggattaa gtaaattata tactgatatg aatgatttta ttgaaaacgt atctacagga
    27601 gatttagaaa aaataaaaaa caattcattt gaaaacgcaa agtgttataa gttagaagaa
    27661 gttaaattat gttccccaat tgtaagacct aaaaaagata ttatatgttt aggtctaaac
    27721 tataaagacc atgtcaatga aatcccagat ggagttataa aaaacgtagt tatgccagat
    27781 tatccaattt atttttctaa gagagcagac aaaataattg gagtggatga taagataagc
    27841 ttacatggag atttggtaga gaagttggac tatgaatcag aacttgcagt aatcataggt
    27901 aaagaaggta taaatatatc aaaagaagat gcttatgaat atatatttgg atatacaata
    27961 gtaaatgata taagtgaaag agcacttcaa gataaacatg ttcaatggtt tagaggaaaa
    28021 agcttagaca cacatacatc tatgggacct tgtatagtac ataaagaaga atttgaacat
    28081 cctttaaaat tagatataag tagtgtagta aatggagaag taagacaaaa ttctaataca
    28141 gagtacttta tatttgatat acctactata ataaatgatt tatcaagagg aatgactcta
    28201 aagccaggag atataatttc aactggaact ccagcagggg ttgctatggg gatgaatcct
    28261 caagtatatt taaagcatgg cgatatagtt gaatgtaaag tcgaaggaat aggagtttta
    28321 aaaaatatag ttgattaatg gataaatatg ccatataatt ttattagtac aaataattta
    28381 attaatatag gattgatcaa acttatgatt aatttattat agagagggat gagtatatgg
    28441 caagagaact agaattaaag tatggctgta atccaaatca aaaaccatca aagatttata
    28501 tgaagaatgg agaacttcct atagaagtat taaatggaaa acctggatat ataaattttt
    28561 tagatgcatt taatagttgg caacttgtga aggaattaaa agaagctaca ggtcttccag
    28621 cagcaacttc atttaaacat gtaagtccag caggagcagc agtaggggta cctttatcag
    28681 atactttaaa gcaaatatat tttgttgatg atttagagct ttctccatta gcttgtgctt
    28741 atgcaatggc aagaggagca gatagaatgt cttcttatgg tgattttata gcactttcag
    28801 atgtatgtga taaggagact gcaactatta tagcaagaga ggtttctgat ggtataatag
    28861 ctccaggata tacagatgaa gcccttgaaa tattaaaagg aaaacgtaaa ggcaactata
    28921 atattgtaaa aatagatgaa aattatactc ctgagcctat tgaaacaaag gatgtttatg
    28981 gaattacttt tgaacaaggt agaaatgaaa tattaataaa tgaagaacta ttaaaagata
    29041 ttccaactgc gaataaaatt tttacagata gtgcaaaacg tgatttaatt atagctctta
    29101 ttacattaaa atatacacaa tcaaattctg tttgttatgc taaagatgga caagtaatag
    29161 gggtaggtgc aggtcaacaa tcacgtattc attgtactag acttgcaggt aacaaagctg
    29221 atacttggta tcttcgtcaa catcctaaag tattaaactt aaaatttaaa aaggatatag
    29281 gtcgtccaga tagagataac actattgatg tatatctttc tgatgattat atggatgtgc
    29341 ttgctgatgg catatggcaa aacttttttg aagaaaaacc agagcctcta actggagaag
    29401 gaaaaagagc atggcttaaa actttaacag gagtagcact tggttcagat gcatttttcc
    29461 catttggaga taatatagaa cgtgcaaaaa gaagtggggt aagttttata gcacaaccag
    29521 gtggttctat tcgtgatgat aatgttatat taacttgtaa taagtataac atagtcatgg
    29581 catttactaa aaatagatta ttccatcatt aaaatactga aagtataaag ataatataga
    29641 ttaagtaaaa atctacaaca agttttgttg tagatttttt tatttgctgt ataattttaa
    29701 atagaacttt atttttagat attcaatttg gaggttgata tggctttaaa tataagaaac
    29761 tatagcctat tatgtaatca aggaacagct aaatttaatg aagatgtagt cggtataaat
    29821 ccatttgggg catgggtttt agatggtgct acaggactta atggaaaaaa tttagtgtca
    29881 aatgaaagtg acgcaaggtg gtatgttcag tggtggaata ggtatttata taaaaatata
    29941 aataaagaag aatcattaaa gagtattatt aatactggaa taatagaagt aaaaaaagag
    30001 tatgaactaa gattaaatgg gtttaaggtt gaaaaattag atttaccatc tagttctata
    30061 gttgttgtaa aatttcatga aaataaagta gagtattttt tattaggaga ttgtgctttg
    30121 tttattaaaa atggagattc caaagttata aaagatagga gtatttgtaa atttgataat
    30181 actgtatttg atgaaatgag taaactttct aacttggaag agatggcatt tgatgagatt
    30241 agaactagtg ttatggatac aatagtaagc aatagattga agaaaaatac gaaagagggc
    30301 tattggatat tggattttga tgaggaagct gtggaaaatt ctatatatgg atacattgaa
    30361 ataaaaaaag attttcaatt gatgctaacg agtgatggtt ttacaagtgc atgtgataga
    30421 tataatctaa taaaagaaga agaattgata cgtatagcag aaaaatttgg tataggatat
    30481 atacataata aagtaaggga ttttgaagat aatgactata aagcggttaa agtgccaaga
    30541 tttaaaataa aagatgattc atcttgttta tatttagata tatataatga ttagactgta
    30601 taaaactatt taattttaga aaggaggcaa gtgaaaagtg aaaattcttc atataataac
    30661 acaaaaacca aatagtacag gtagtggtat atatttgtct ggtatgataa aagggttcga
    30721 aaaaatcggt cataagcaag ctgtaattgc aggtatagat gtaaatgatg atgtaaattg
    30781 ttttccaagt gaagtaagtt tttatccagt aaagtataat tgtggggagt taaattttcc
    30841 tgttgtaggt atgagtgatt ctatgccata tgaaagtaca agatataaag atttaaatat
    30901 agacatgata aatagattga aatatcaatt taaagtaaat atagataagg ctatgaatga
    30961 ttttaaacca gaacttataa tatgtcatca tttgtacctc ttaactgcat ttgttaggga
    31021 aatagtaaaa gatataaagg ttatgtctat ttgtcatgga acttgtttaa gacagttaaa
    31081 taccattgat ttagagaaag aatacataat agcaaatata aggaaattag atttaatatt
    31141 tgctcttcat gaaaatcaga aatatgatat tataaagact tttggagtaa gtgaaagtaa
    31201 agtagttgta attggtagtg gttataatga tgatatattt tataataaaa attataaaat
    31261 aaaggatgat aaaataaaaa tagtatttgc aggtaagata tgtaaatcaa agggattgat
    31321 tccatttata aaatctatat ctaaattaaa atatagtaaa gatttaatag aagtcaattt
    31381 tgcaggtact ggaagcgata tcgaatcata taatgagata gttaaactgg caagtaagtc
    31441 tccatttaaa atgaatttcc taggaaagtt agaacagaga gatttagctg agctattcaa
    31501 caggtcacaa atatttgtgt taccatcatt ttatgaggga ttaccagttg tagttttaga
    31561 agctttatct tgtggcacag atgtaatcac tactgatatt cttggtgtta aagagtggat
    31621 tggtagtgag attaataatt ctggaaagat agaatatatt tctctacctt ttatggagaa
    31681 agaaggtatt ccaaaagatg aagagttata tgattttgaa aataatttgt ataatgcaat
    31741 agattctaag atacaatctt tattgaataa ctcaaatgag aaaacatctg tagatatgtc
    31801 aaaaaaaacg tgggatggct tagcatatag aataaatgaa gttattttaa tggatgaatt
    31861 gtgtttagtc taaaattatt taaattataa aaaagtgtaa ctatttttaa acttttttta
    31921 aatgatttta tattatacta atactaaata tatttgtttt agggaggtaa tttatgagga
    31981 ttagtaaaaa ggtattggcg ttaggtattt cagcattact gttaagtgtt agttttccaa
    32041 catcaataaa cgcactagat aagattgaaa atattcaagg agtagataaa tatgaaacag
    32101 ctgggcttat ttcagataag caagatttta caacagcagt tcttatcaat gcagattcta
    32161 caatggtaga tgggattgct gcaagtggtc ttgcaggagt aaataatgca gctattttat
    32221 taactaataa ggatgatata ccagaagcaa cacttcaaag actgaataga gttactaaga
    32281 tatatgtaat aggtggagaa aattctataa gtaaagatgt agaaaaaatg ttacttatga
    32341 gaagaatgca agttataaga attgatggtg tagatagagt agatacaagt tataaaattg
    32401 caggtgaaat agaaaaaatt aaaaacagtg ataagatgtt tttagtaaat ggatttaagg
    32461 atgaagctga tgcagtaagt gttgcatctg ttgcttatag agatggagct cctataatat
    32521 tgacaaaaga tataccttca gcagaagaag acacagactt agacccatgg tttggagttt
    32581 ctcctgttcc agtatatgct attggaggag agtctacatt aagtgattac atagtgagca
    32641 gatatagagc aactagaatt ggcggtgttg atagatatca aactaataaa aatgttattg
    32701 aaaagttcta taatggagca aaagagttct atataacaag tggagacgat ttagtttatg
    32761 ctttagttgc atcaccactt gcaaaaaatg ctcctgttgt tttagtttca aataaaagtg
    32821 ataagagtat attaagtgga gctagtaaag taacagcaat aggtataagt gataagagta
    32881 ttatagagca atgtttggat gctgtaaaga agtaatggag tgttattagt aaaatgaata
    32941 tatacattga aaattagtag attgttcatt ttataatggt ataaaatata tgggaagagg
    33001 ctatcaaatg gagataagcc tcttttttat tgataaaatt atgaaaatat actttatttt
    33061 atataaaaaa agtatctcat gaactaaatg tctattttga tatactatct ccttttctta
    33121 tattatatgt attatatgat tttttatatg gaataaattt cataagtatt atcttccttt
    33181 aatttttcta accaaatcat ctctaaaata tctagttctt ctaaatagtc tttctctgtt
    33241 ttgttatcat catgttttat aagttctaaa acttcaaatg taaatgcttt ttcaccatat
    33301 tttttccatt cattatttaa ttcttttatc atacatgagc cagcaccaag ctgaaatttg
    33361 agagagttaa atttgctttt tatgtcatta ctttttccaa ggtaaatctt tttagtaata
    33421 tcatttttta ttatgaatac acctgtgtca aatttcattt ctttgtataa ttgttttaat
    33481 tcacgttttc tatccatgat tttagatacc tctctttata attgatatgt aatatgtaag
    33541 tttaaaaata tattacatat caattataaa gagattaatt ttatatgtaa atagttttta
    33601 taaatttaat tatgaattag tcatcaataa acaagaaatt tagctgtaga aatccattat
    33661 aaaggctatt ataaatttta atatgctaaa aatataaaaa gaatagttga cagtatagaa
    33721 aatatggaat aaactaaaag tgtaatagta atacgaaatt atatattaca ttgaaaggtg
    33781 tgaatgatta ttatgaataa aaaaatagaa ttaataggga attttgcaag tgattggatt
    33841 aggtatagtg attatgaata taaaattact gaagaagatg aattatatat agttccaaca
    33901 gaagatgctg tgtttaccat gtataaccca tttgatgtag cagatgatat aatagtagat
    33961 ataattcgca taggacaaga ggcattacaa gatatatcaa aagagacaaa agaaatgaaa
    34021 ctaaagaaag aagtacttga atttgtaaaa aagtatggat tattgggttt aatatattca
    34081 agtgtatata atagagatat agttggagaa gaaagagtac tgatgattga aaagaattat
    34141 attacaaaag agaatatatt gagtacagat gaatatgtaa acatgtttat accatttgca
    34201 agtgatgaag atgtgtattt taaaaaatat agaaggggta tagatgtatc aaagagagaa
    34261 gaatctccaa agttttttgg aaagagacca ttgattttag atttagtatt ttcaaagttc
    34321 tacactgaac aactagattg gattgtaagt tttgctaaag atatgtgtaa gcattttaat
    34381 caaatttgtg tatataaaaa tacatctaat ctattaactg aaagtgttac tattatgtct
    34441 ggagggttta atcctcaaaa gataggattt tctataagcc aattagataa accaattatt
    34501 gcatgggagt ttgattcttt gaaaacagct attgagacta tctatgcctt tgcaattaca
    34561 agtgaacctt cttctttaaa taaatgcaag tactgtggta aaatttatat tcctacaaat
    34621 tctaaatctc aatactgttc accttcttgt agaaattgct caaatgtaaa gaaaagtaga
    34681 agtagagcca aaaaagataa ataaaattaa taaatatatc attgtttaga ttttagtaaa
    34741 taactactga attaaaagtt gttatagaaa attatatatc taaatgatgg tatttttttg
    34801 ttgagaaaat tgaaaagttt aattgagata gtaattttga gttgacaaaa accactttta
    34861 acactactat tataagtggt tggtaaaata atatacacta atttaaatat agcataaaaa
    34921 acaaaaaaag tcaatgaaaa aaataaaaaa ggagaattta aaaatgatat cacctattat
    34981 tttgagttct ataaatcaga atttaaagga aatagaaaga aatgaactgc ttgaaactaa
    35041 tatagaatct ggagattatg gtctagctct ttctgaatct gatgtaaaag atataataaa
    35101 ttcgagagat aatactttaa aggggtatgg aagaatagaa ttagatataa aagttacaaa
    35161 acagttaata gaaaatatat atacatcaca atatacaaat gtggacaatt atttagaggc
    35221 tattaatgat atgcaagaaa ttttttatta tttaaaaaat gaaactgatg acaagatatg
    35281 tgatgatgag attatagaaa tactgggtga attttatgaa aaattttcag gcaatatgga
    35341 caatgtaaga ggagaagcag acgaatttgc aaagaaattt aaatttggag aagtttaata
    35401 tatgatagga gagattttgt atgttaaata atttaagtaa tattaatata aacgaaaata
    35461 atttaataaa gaaccaatat tccatatctc tacttaagga atgtctaaat tgtaaagtga
    35521 tagatgaaag agaagtatat aatatacaac aagaaatatc tctaatactt atggatttga
    35581 ttaaaaaata tacaaatggt caaagcacat cagtaaaaac agaagttgcc gaaaaactgt
    35641 tgatttcaat atggtatgct attgatgcat atataaataa gcttggagat atagaaaaaa
    35701 gagttgtgga gttgactgct aagaatgtta aaaaagtgta ccttggaggc attgaaatat
    35761 tagaggaaga gttagttagg ctgaaagatt tttataaact aatgatgagt agaaaattgg
    35821 atactaattc aatagcatat aatgatacct tgatggagtt atcaagtttc tttaaaagtt
    35881 atattgtaaa atttgaagct caggacgttc cagtaagtat agattatcca ttggcactag
    35941 atgatatgga tgttaagggt atttattatg tgaagaatta tattgagagt atagatatag
    36001 aaaatagatt ttgtaaccta tttaagaaaa aagatagaga aaaattattt tatgactatg
    36061 gggtgattta taaaatagat tgtgatgtta tacttgtaaa tttatttgaa cttattataa
    36121 ataattgtat tttttctact attttaggaa atgaagtgat taatttagaa atttcaaaat
    36181 atgagtatga atttttagaa aaacaattta aaaatatgta caataatatt actgaagata
    36241 aagttatagg ctctgaagaa agtaatataa ttgaattgat tgaagtagat gatagtttta
    36301 acactgaagt agatagtaat ttggattata atgattttga agaaattaac agagaaatta
    36361 gaataaataa aaaaataact ttactactta tggaagcaat tgaagtatta ataaaaagat
    36421 taagcataga agatgaagat gtaataagat atattaaaaa atatgaaaag atatttattg
    36481 aatctgtgct aagctcaata aaaagtaaca cttttaaaag tatggtaact gtgagtaaaa
    36541 ataaaaatga acacttagaa gactatattg ttgatgatga aagtaaattg gatgatgaat
    36601 cgtttagaga gatattcaat gagatattag atagtacatc tataattgaa aaaataagaa
    36661 taataaagga aaatatcaat gctaaaaaag actttatcga tattcttgaa tctgaatgtt
    36721 tatttggaga agagtattta atgttgtttg catcattaag tgaattagaa ttggcaatat
    36781 tgggtagtgt agtattttat gaagatatta gaatgagaga aattaatata ttagaattta
    36841 tgttaaatga gaaaactgaa actactttat ggaagacaga gtacattaaa tttatgaaga
    36901 ggcaaagtga agataagata tactccatag aagagcatat gaataaaatt aattaacaaa
    36961 aaattgtgaa taaatagaat gttagtatat aatataatct gtaaagcata tcaaaaatac
    37021 atatatttgg aggaaaatta tgaaagagta tatcataaat aatgttgaag aaataagaga
    37081 agaattaatt gatttatcta agaaaatatg ggaaaatcca gaattagcct ttgaagaaaa
    37141 gtatgcaagt agcattcaaa aagaatacct aaagtcaaaa ggatttaaaa ttgaagaagt
    37201 agaaaattta cctactggat ttatagcaag ttttggagag ggtaaacctg tgattggcat
    37261 acttggagaa tatgatgctt taccagagct atcacaatgt gtgagtgcag aaagaaagcc
    37321 attggtagaa ggaaaagctg gacatggatg tgggcataac ctgttgggtg tagcaggtgt
    37381 aggagcagtt gtttctataa aaaaattaat agaagaaaat aaatttaatg gaacaataaa
    37441 atattttggt tgtcctgctg aagaagaagg tggaggaaag acagttatgt gtataaatgg
    37501 ctgttttgat gatgtagatt gtgcatttac atggcatcct tttgatataa atgcaccttg
    37561 gagaggtgga agtctggcaa acttatctgt aaaatttaaa tttaaaggga taacagccca
    37621 tgcagctcaa gctcctcata atggtagaag cgcacttgat gctgttgaaa ttatgaatgt
    37681 tggagctaat tatttgagag aacatgttat tgactctata agaatgcact atgttataac
    37741 taatggtggt ggaagaccta atgtagttcc tggatttgct gaaagttggt actttattag
    37801 aggtaaaaaa gcaagggatg cagaacatgt tcttgataga ttaataaaag tagctcaagg
    37861 agcagcaatg atgacagaaa ctgaaatgga gtataaggtt acagatggaa tatatgacta
    37921 tataccaaac caatgtttga ctgatttagt ttatgacaat atggtttttg ttggttgccc
    37981 taaaaatact cctgaagaag aagaatttgc aaaaaaattg tgtgatactc ttactagaga
    38041 agaaagatta ggtgtagcaa catcattaca aaatgataaa tctattgtag aaagttatat
    38101 acacaaggaa atagttgacg gtgacaaaga taaaggacta gctggttcta ctgatgttgg
    38161 agatgtaagt tatgtaatac cagtggctca atttgcaatg gcagcttggc ctgttggaat
    38221 tgcaagtcat acatggcagt catgttcatc agcaggttca aatataggat tttctgctat
    38281 gataaattca gctaaagtac tggcttgtag tgcttatgat gtatttatga atactaaaat
    38341 tattgatgaa gcaaaaattg aatttgataa atcattagat ggacaaaaat ttaaaccatt
    38401 ggtataattt tggtaataaa atatttctgg ggggaatagc tatgagagaa aaagttgaaa
    38461 aagtgcttga ggaaaagata aaaccagtat tacaaagaga tggtggagat gtagagctta
    38521 tagatgtaaa tgaaaatggt gtggttttag ttagactaca aggggcatgt agtgggtgtc
    38581 caggagcgac tatgacaatc aaagctatta ttgaaaatgt tttggtgagt gaagttccag
    38641 gagtaactca agtattaggt gtttaatagt tagcgtttat taaatgtatg ttatacataa
    38701 gtatttaata aagaagtaaa aaaaatagtg aagcaatata tatgaaaaag tatgataaag
    38761 ataataaaac attatagaaa taatgaaata tttcatatgt atatgaagaa ataagaaaaa
    38821 atagtgaagc aatatatata tgaaaaagta tgataaagat aataaaacat ttatagaaat
    38881 aatggaatat ttcatatata catatgaaaa aatagtaaaa caatatatat atgaaaaagt
    38941 gtgagaaaat aataaaatac tatagaaaca attaaatatt ctatataggt attaagaaac
    39001 atgaaagaaa taatggagta ttatagataa atatgtaaaa gtgtgaaaga aggatgtaga
    39061 taaatttgtc ttatacaaat ttacctacag tatttcttac acacttttta ttttactatc
    39121 caaccatacc aataaacata cctataaagt atggaactaa ccatatagtc catacaacta
    39181 tactaaactt atgaaagaat tttttctttt cctcatcatc tttgtatagg acaaaagtag
    39241 cccaacttgc atgaaaaagc ataagcacaa ttgcaagaag acctgtaata ctatgaaggt
    39301 tttgtgataa agcagatttt acttcaaaag tatggctgtt ggctatgtta ctcattgtaa
    39361 atgttcctaa agtatcaaat ataaacccaa gccaaaatat aattacatgt ttctttttta
    39421 aaatttttgc ctttctttct ccaaatacac ctatagtgta gaaaattaaa gctgatgtta
    39481 taaatactat tgcaagtatt aatttataat tcataattat ctctccttta cttttgccaa
    39541 tatatttcta agatttttta ctaagtcact caattcgttt tcatcacaat ttttaaaaat
    39601 atcatcaaaa ctttcttgca ttttaatttt aaaatttgtg gcaaattcaa atcctgttgt
    39661 tgtaaactta acgtatgtat ttcttttatc attagatttc ttgaattttt ctatatatcc
    39721 aatctgttct aatctgctga ttatgcctga aaccgtacct ttagctaaag acatttcatc
    39781 acatagttgt gatattgtaa gttcttgatt atgagctacc agttttataa ctataatctg
    39841 ttggtgtgtt aatccatttt ctttaaaatt ttcttctact gtgtacatag ttttagaata
    39901 aagttctttt aagagcatgg ctattctaaa acagtcataa ttacttttca ataaaatcac
    39961 ctcttaaatg gtttgcatac aaactaatat aatatacttt tcttatatag tcaacctaaa
    40021 attttataat tttatagaaa ataatgaaga atagaacata aaaattttat tttaggtata
    40081 aataaaataa tttgatgaaa ttttaacaat ttttaaaaag tttgtttaca cagttaataa
    40141 atgatgctaa aattaactca tagataaata tagaaaattt aattttgttt aatgataatg
    40201 gaaccacgaa ggttttaacg ttactttacg tttgctttta tgtggttttt ttattgcaac
    40261 aaaggggggt tggggtttgt taaaggatat actgaaaaag ttgttgcagt ttattttagt
    40321 aatgttttta ttatctttta ttgtttttta catggcaaga cttgctccag gagacccatt
    40381 aatctcttac tatggggatg gagtagaaag aatgagtaca caagaaaaag aaaatgctat
    40441 gaaaaaactt ggtttgaatg aaccaatata cagtcaatat ataaaatgga tttcaaatgc
    40501 atcaaaaggg gaatttggaa tttcatttaa atataaacaa aatgttactt ctgttataaa
    40561 tgatgtctat ataaacacta taatattagg tggttcaggg tatatactta cctttgtgct
    40621 agccttattg ttaggaatat tttgtacatt acatgaagat aagctcattg atagaatcat
    40681 atgcaaatta ggaacaataa ccaactgtat tccatcattt tgggttgcct tagtactcat
    40741 acttatattt agtataaact taagtatact tccaagtagt ggtgcttatt caatgggaga
    40801 agagtccagt atattaagta ggatatcaca tctaatctta cctcttacag tacttatatt
    40861 aagccatcta tggtattaca catatatgat aagaaataaa ttactagagg aaataagaga
    40921 agattatgtg cttttatgta aagcaaaagg tttaaataat agaactattg tttttaagca
    40981 ttgcctgaga aatataatgc catcatatat tagtataatg gccatttcga tacctcatat
    41041 actaggagga acttatgtag ttgaaaaagt tttttcttat cctgggttag gaacactttg
    41101 ttttgaaagt gcaaagtatc atgattataa tatgttatta gttttatgct taataacagg
    41161 ggctttagta gtatttggga atatgttagc tcaaattata aacaataaaa tagaccctag
    41221 aatgaaatat gatagaggtg atactaatga agccacaata tagtgatttt gaaattgtag
    41281 gagaaaatta tattttagta gcagaagatg aacatataga aacaaagaaa accttatcta
    41341 aaaaaattaa agaattacca tatatatcaa ttattatttt atcaatcata gtcattggaa
    41401 gtgtattctc atcttttata atgacacatg aaccaacata catggattta gcaagttcta
    41461 atttagctcc aaataaaact tttttatttg gaacagactc aatgggtaga gatatatatt
    41521 ctatgatatg gtatggagga aaaatatctt tatttatagg actattttcc actgtaatat
    41581 caactgttat aggaattgtt tatggtagta taagtggttc agcatctgaa ctcgtagatg
    41641 atgcaatgat gaggtttaca gaaatcatac ttagtatacc ttcaatatta attataatat
    41701 ttgtacaagc tatacttgga aattcaaatc cagtatctat gtccatagta atagggataa
    41761 caagttggat gaatatatct aagatagtga gaacagaagt tagacaaata agaaatagcg
    41821 aatacatact agcagctaag agcatgggag gaggattctt ctatgtatta aagcaacatt
    41881 tacttccaaa ttttgtggca tcaataatgt tcatggttgt aacaaacata ggcgcagcta
    41941 ttggtactga atcgacactt agctttttag gaattggttt accaatagaa attgtttctt
    42001 ggggaagtat gttatctttg tcagaggaag cattactttc aaatagatgg tggattatac
    42061 tgattccggg tatatttttg gttaccacat tggtgtgtat aacaaatata ggtaattata
    42121 taagaaagaa taataataaa aaatctagta atttataaat cagcatttga gtactaaata
    42181 agcaaatact gaggtatttg aaatatctat ttaaataaat ggggtcaaag ctttgaaaaa
    42241 tattttataa aaagcaaaag ggggagcatc atgaaattaa agaagttaaa agttttaagt
    42301 ttagtaatga tacttagtct aatggcaggt tgttctagtg gaggagacaa agataagaag
    42361 gcagatacgc ctaaagatgg aaaggtactt gtttatggaa gtaatgacta tacaagtata
    42421 aatcctgcac tatatgaaca tggagaaata aattcattaa tatttaatgg attaacagct
    42481 catgatgaaa ataataaggt ggttccttgt ctagcaaagg actggaaatt tgatgaggca
    42541 acaaatacat atacttttaa tttaagagat gatgttaagt ggcatgatgg agagaaattt
    42601 acggcaaatg atgttaagtt tacaatagaa actataatga atccagataa tgcttctgag
    42661 atagcatcaa attatgaaga tataacaaaa attgatgtgg ttaatgataa tactataaaa
    42721 attactttaa aagctccaaa tacagcaatg cttgattatt taacagtagg agtattacca
    42781 aaacatgttt tggaaggtaa ggatatagct actgatgaat ttaatcaaaa accaataggt
    42841 actggaccat ttaaacttga aaaatgggac aaaggtcaaa gtataacact tgttaagaat
    42901 agtgattatt ttgtaaaaga accaggatta gataaagtag tctttaagat tgttccagat
    42961 gataaggcta aggcaatgca attaaaatca ggagaattag acttagctca aataactcct
    43021 aaagatatgt ctaactttga aaaagacgaa aagaatttta aagtaaatat aatgaaaaca
    43081 gccgactata gaggtatatt atacaatttt aattctaaat tctttaaaga taaaaaagct
    43141 aaaggattac caaatgcttt aagctatgca atagatagaa aagcaatagt tgacagtgta
    43201 ttattaggac atggtgtacc agcatattca cctttacaaa tgggaccata taataatcct
    43261 gatatagaaa agtttgaata taatccagaa aaagctaaac aagaaattga aaagctaggt
    43321 tggaaattag gttctgatgg aatctatgaa aaagaaggta ctaagttagc tttcgagata
    43381 acagcaggtg agagtgacca agttagagtt gatatggcta aaatatgtgc acaacagtta
    43441 aaagaaattg gtgttgatgc taaagctgtt gttgtaactg aaacagattg ggctaatcaa
    43501 gatgctcatt taataggttg gggaagtcca tttgacccag atgaccatac atataaagta
    43561 tttggaacag ataaaggtgc aaattacagt gcttactcta atcctactat agataaaata
    43621 cttcaaaaag caagagaaac agaagataaa gatgaaaaat taaagttata taaacaattc
    43681 caagtagaaa tgactaagga tatgccttat acatttattg cttatataga tgcaatatat
    43741 gttggaaaac caaacataaa aggattaaca cctgatacag ttttaggtca tcatggtgta
    43801 ggtatattct ggaacatagc tgactggaca attgaataat actgaaatta attagaaacc
    43861 ttagggataa aaatatatcc ctaaggtttg aatagcatag gggttgatgg tttttggaac
    43921 atttattgga ggttaataat ctatctgtaa gttttaaagt agaagaagga gaagttcagg
    43981 ctgtaagaaa tgtatctttt aatctaaaaa aaggtgagac attggctata gttggagaat
    44041 ctggttgtgg gaaatctgta ctgtgtaaga gtttgatgag gatactccca tacaatggat
    44101 atattaaaaa tggagaggtt ttacttaaat caagtgattt ggttaagaag tctgaaaaag
    44161 agatggaaga tataagagga aagaatatat caatgatatt tcaagaccct atgacatctc
    44221 tcaatccaac tatatcaata ggaaagcaaa ttgcagaagc tgtaatcatt catcaaggta
    44281 taagtaaaag tgaagcaaaa aagagagcca tagagcttat tgaattggtt ggaattgata
    44341 atcctgagaa aagatttaaa caatttccac atcatttttc aggtggaatg agacaacgta
    44401 tagtaatagc tatagcactt gcctgtaatc cagatgtatt gattgctgat gaacctacaa
    44461 ctgctttaga tgtaactata caagctcaaa taattgattt aataaaggat ttacaacata
    44521 aaataggttt gtcaataata tttataactc atgatttagg agtagttgca actatagctg
    44581 atagaatagc agttatgtat gcaggaaaga ttgtagaaat aggaactgta gaggatatat
    44641 tttatgaccc tagacatcca tatacttggg ggttgttggg ttcattacct actttagatt
    44701 cacaggatga atatttatat aatatacctg gaatgcctcc aaatttattg aacccaccaa
    44761 aaggagatgc ttttgcaata agaaataaaa atgcacttaa gatagattat gaaaaagagc
    44821 caccaatgtt taaaattaat gatacacata gtgcagcaac ttggttattg cacccagatg
    44881 cacctgaagt agatgtacca gttagagtaa attgtggaag ggtgatttct aatgaataga
    44941 gaaaaaatac tggaaataaa aaacttaaag caatattttc atttggacaa aagtactact
    45001 gtaaaagcag ttgatgatat aagttttgat atatataagg gtgagatatt tggattggta
    45061 ggagagtcgg gttcaggaaa atctacaact ggtaaaacta taataagact acatgaatct
    45121 actggtggag aagtaattta caaaggaaat tgcatatcag ataaaaaaac gtacaaattt
    45181 ataaaaaaag atgtaaacaa aagtatgcag attatttttc aagattctac ttcatcatta
    45241 aatccacgta tgacaattgc agatataatt tcagagccac taaaaattca aggtatctgt
    45301 aaaaataaaa cagatagaat gaataaagta tatgagatgc taaagctagt aggacttgat
    45361 agaagttatg caaataagta tccatctgat ttttcaggtg gacaaagaca gcgtattggc
    45421 atagcacgag ctctttcagt agacccagaa tttataatag ctgatgagcc tatagcttct
    45481 ttggatgtgt ctatacaagc acaaatagta aatttattta aaaaattgca acaagaaaag
    45541 aatttaactt gtttatttat agctcatgat ttatctatgg tgagacatat tagtgaccgt
    45601 ataggagtta tgtataatgg aaaattagta gaattggcag attcaaatga attgtataat
    45661 aatcctattc atccatatac aaaatcacta ctttctgcaa ttcctgttcc tgaccctaga
    45721 tatgctaagt caagaaatag aatagagtat aattctaatg gatatgattg ttcaaatgaa
    45781 aagtcattaa gttggattga ggtttcagat ggacattttg tatattcttc taaatcagaa
    45841 ataaataaat atcaacaaaa tttaaaagtt gtttagtata aaaatacttg aacaggtatt
    45901 tatttgaaat acaaactaaa accctatttt tggagaaatt ttaaaactac tccaaaatag
    45961 ggttttttat ttctactttt caaatataat aaattcaaat ataataaaat taaaaacaag
    46021 taaagtatgt tgttaagaaa gtgctataaa attaaaaata gtaatgtaga aaaaatgagc
    46081 aataatctta ttttttctac aataattttc tataatttaa agtaaggaaa gtttgataaa
    46141 ttataacctt agttaaagaa taagagttta ttaaaaaata aagtgtgtat aggggtttta
    46201 gttaataaaa tattataatg taggggagct tgtaatgata tataaaaaag ataaagtaat
    46261 gaaagtttta gaatcatcat atagggaaaa tgaaaaaggg attacagcta accaattatc
    46321 agaaaagaca ggaattgcaa gaaacaatat aagtacatat ttaaaccaat tattcaatga
    46381 gggtatggtt acgaagataa aaggaagacc tgtgtattat atccctgcaa atcttaacct
    46441 taacaagaat gaattgcttg atacacaaag tgagttgctt ggcataaaaa aaggaacaaa
    46501 agtagaaaac aaaataaaaa aagatagttt tgaaacacta ataggtaaag aacatagttt
    46561 aagacctata atagaaaaat gcaaggtcgc aatgctctat cctacaaatg gacttcatac
    46621 tattatctat ggagaaacag gagtaggaaa gagtatgatt gctagatata tgtacgatta
    46681 ttctatagat agtggaatta gaaaaaataa agctcctttt gtaacattta attgtgctga
    46741 ttatgctaat aatgcacaat tacttatggg acatattttt ggagtagaac aaggagctta
    46801 tacaggtgca gaatcttcca gaaaaggtct tttggaaatt gcaaatggag ggatactatt
    46861 tttagatgaa attcatagac taccagcaga aggtcaagaa atgttgttta cttttataga
    46921 taagggaaca tttaaaagac taggagatgc aacgaaagaa agagattcta aggtattaat
    46981 aatatgtgct actacagaaa atccgacatc aacactatta gatacattca atagaagaat
    47041 accaatgaaa atagaaatac catcactgag agataggaca atgattgaaa gaatggaatt
    47101 agtcaaaaat tttttcaagg aagaatcaaa aagtatttct atttcaataa aagtacataa
    47161 agaaataatt aaatcactat tactttatga ttgtaaaaat aatgtaggtc aacttcaaaa
    47221 tgatataaaa ttagctgttg caaatggata cctaagatat aaaagagata ataaagaacc
    47281 agtttatata gagaaaaaat attttgataa atctataaat gaagtgagtg atgattatag
    47341 agagaagtct attaaagcag atgaagttat aacacatgga atagaatatt ttgtatttac
    47401 gtcattagga gaagaagaag ttattaacta caatgatata aatattatta atgcagtaaa
    47461 tactaatctt aataaatctg tagaaatgaa tatagcagat gcttctctat ttaataatgt
    47521 taagtttaaa agcatgtgtg aatcaataag acaaattatt aaagaagatg aagaaataga
    47581 tataagtgaa tcaatattta agtcaatggc aatttatata aaaagcgtat tggataaaag
    47641 tagctctaca aacaacatag atttgaatca aattagaaga aataacagaa gtgaatttaa
    47701 aacagcacta aaaatagttg gaataattga aaatgaattt gatttatttt tatctataga
    47761 aactgtagca tatattacat tattcatagt acaagctaaa caagatgaac aaaataaaat
    47821 agtgaataac attaatatta tagttgctat gcatggagaa actacagcaa gtaatggtaa
    47881 aagcagttgg tgatattatt ggaaagtgca gtgctatttc tttcgatatg aagttagata
    47941 aaagctatga tgaagttatt gtagatttta aaaatttaat taataatata gacaataaag
    48001 atgtattact atttacagat atgggttcat taaactcctt tgatgaaatt ataaagaaag
    48061 aaaaaaagtg tgaagtaaga gtaattccta tggtgacgac attaactgtg ttagaagcag
    48121 ttcagaaggc caatatggga ttacctttaa atgacgtata caactctatc actaacacga
    48181 gaaagtatta ttttggaact aatgaaattc aaaataaaga aaatctgtct aaaacaatta
    48241 ttatagcatc acatgtttca gaaggtgtag ataataaaac aagaaaaata ttagaagaaa
    48301 agatgagtag gtacttagat ggaatagata ttatatctgt cccatataaa acagaaaaag
    48361 atttaagttt aaatattaca aaacttaaag aaagtagtaa tattgttgca gtaatcaatg
    48421 aacaaagaat taatattaga ggaattgact atattagcaa aaaagatata gataaggatg
    48481 aaaatatcaa taaattaaaa aatattataa agataagtat aggttatgat gatgtagttg
    48541 aaggtttaaa gacatcctta aaaagctcta attataatag gatatttaaa gatataaaat
    48601 atgtgtctga tgaattgttt ttagtattta atatagaaaa gaaatacgat aaagtaattg
    48661 gacttatgat gcatttagct ttcatggtag atgggttgat tggaaatact agagaaattg
    48721 aaaagcttga taaagaaaaa acacttgatt atcacaagag cctaagcaaa ataaaagata
    48781 tagtatctca attagataaa aaatataata tagaaattaa tgaaaaagag tgctatcaaa
    48841 tattattaat attagaatat gcagaaataa tcgaaaaaga ttaccaatga gtataaaaac
    48901 ttatttggta atttttttta gtctaaaata atattaatat tagacacata taaatatact
    48961 tagacaaagt atctaagttt tgatgataaa aatgattgaa ataacagtgt tttaaagtgt
    49021 ctaaaaaaac tggcataata cttgcttata ttattaaatg agtaatataa ataattaaaa
    49081 tttaaataaa aaggagtaaa taatatgcaa gataaaatat taagagataa aaaggcaaat
    49141 gacccatggg caagaatgac tacaagaaac ggatttagtg cagacgaaat aatatcgtca
    49201 tttcaaaaag ctataagaag aaatatggtg gaagaagctt gtgaatttgc ttatgaacta
    49261 tatatatcat caccagaatt agaagataag ctatggagaa ggatacttac aatatcagtt
    49321 gaagatatag gaatggggga cccatcagct gtaataatca taaataattt aaatcaagta
    49381 agaaaggaat attcatatgc agatggagac agacctttat ttttcataca tgcaataaga
    49441 tatctttgtg catgtgagaa agatagatca agtgatttat taaaaaatat aattataaaa
    49501 ggatttgcaa tgggtaaagt tccagaagtt atggacgtgg cacttgacaa gcatacatat
    49561 agaggattag aaatgggaag ggattcattc cactttttaa atgaagctag tatagtaata
    49621 ccagaaaaag aagtagataa tgattataaa gaaagatact taaaaatatt agaaaaatat
    49681 aagcaagaag aagttataga tacggctttc aagtttaatc attggcagta ctaagaatta
    49741 gagtaaatat taataagaat taaaataaaa taataagaat taaagtaaaa actgatagga
    49801 attagagtaa gaattggctg ataaaaaatt agaaaataaa agaggttaga tattgtagga
    49861 gggtaattat gaatatttta ttagtatgtg caaatggagc ttcaacaggt gtacttgtgg
    49921 aaaagatgaa aagtttttgt agtgagcatg aaaagttaaa aactaaaact ataaatatag
    49981 aggcaacatc attcgaaaat ttaaaatcat acattgaagc taatgataca gatgtagtac
    50041 ttgtagcacc acaaattagg ttcaaggaag atgatgtagt tgaagcttgt aagaattaca
    50101 aaatagcagt tggtttaata gatactaagc attatggtcg tatggatgct ccttctgtta
    50161 tgaagtcggc aatcgaatta tataaaaata gataattggg gggaataaaa atggaagcac
    50221 ttcaaaataa tttaagaaga tttcttctac caatagcaca aaagattgaa aaacaaaggc
    50281 atcttcaagc tataaaagaa ggtatgataa gcattacccc aataataatt gttggttctt
    50341 taagtatgtt atttatggca ttaaacaata tgttacctga aggaagtgca aaaacattat
    50401 tatctgaaaa tatggataca ttacttatcc ctaataaatt tactatgagt ttattatcta
    50461 tatattcagc attttttata gctcaggcac tagctaaaaa atataatctt aatcatgtag
    50521 aaataggtat gacagcagtt gttgcacaac ttgttgtatg tggacaagta gtagatggtg
    50581 tattagatac aagttattta gatgcacaag gtttatttgt aagtatatta gtagcacttt
    50641 tagtagttga tattactaag tttatgaatg ataaaaatct cgtaattaga tttccaaaag
    50701 aagtacctag tgtagtaaat aaaagtttta gaaatttaac tccaatgata gtatgtataa
    50761 tgttatttac agcaatagca gcaataacta aaaatgtctc aggtcaacca ttaccagcta
    50821 ttataatgaa tttcttagca ccagctataa gcagtgtaga taatgttttt gcagtaacta
    50881 taatactatt tataactcaa ttattatggt tctttggcct tcatggagca gcaataactt
    50941 caagtatatg gatgccaata gcagctacat atatggcaga aaatgcaaca ctgatagcag
    51001 caggaggaga cccaaaatat gtgtttacaa taggatttta ctatggtttc ttgcaagtaa
    51061 ctggttcagg gataactttg ggattagtgt accttatgtc aagaagtaag tgtaagagtt
    51121 ttaatagtat gggaaaagtt gtgattttac catcactatt tggaataaat gagccagtaa
    51181 tatttggaac accaatagtt atgaacccat atatgtttgt accatttgta tttggacctg
    51241 tattggttgg agcacttaat tttatggcat taaaagtagg attagtagga ttacctattg
    51301 cagaaccacc aggcttttta cctccagggg ttgcagcatt tttgatgaca ttagattgga
    51361 aggcaatcgt attagtattt gcaagtataa tacttatgac cttgatatat tatccattct
    51421 ttaagataat ggagaaggaa gaattaaata aaaatgcaga attagctaca acattagatg
    51481 atgatagctt tgatttttaa caagtcatta gaaggagaag gatatggtac ctgaaatgga
    51541 agaaaaagta tttagtataa tatcagaagc aggagatgct aaaagtgatg taatgatgtc
    51601 tcttaaggaa ataaaaaaag gtgactacaa taaagcaaaa caccttttaa attcagcctc
    51661 agagaaaata caaacagcat caaagcatca tttagaatta ctatcaaatg ctatgaatag
    51721 tgaagatagt gggactgatt ttttagttgt gcactctgaa gaccattatt caaatgcatt
    51781 atttgctcat tctttagtat ctgaacttgt agatatattt gagatgatgg acccaagaat
    51841 taataagaat taaatttctt gtaaatagtg cttgaagttt ggctatagtt tattaaatca
    51901 atagatttaa taaatgaact cccttttact ttaaagtact agtatttata aatttattta
    51961 taaatacagt aaatttttaa atagaaaagg agatattatt atggaaaaat tttcagtagt
    52021 aatagctgga ggaggaagta catatacccc agaaatcata ttaatgttgc tagataactt
    52081 agataggtta ccattaagag caatcaaatt atatgataat gatgaagaaa gacaaaataa
    52141 ggtagctaaa gcttgtgaaa tactaataaa ggaaaaggac cctaatatag agtatttagc
    52201 aacaacatgc cctaaagagg cttatacaga tgtagatttt tgtttagctc atataagagt
    52261 tggaaaactt gaaatgagag aactagatga aaagatacct ctaaaacatg gagtagttgg
    52321 tcaagaaact tgtggaccag gtggaatagc ttatggaatg aggtctatag aaggtgttct
    52381 tgaaaacata gattatatgg aaaagtattc tccaaattgt tggatgttaa attattctaa
    52441 tccagcatca ataatagcgg aagctgttag aaggttgaga cctaattcta gggtaataaa
    52501 tatttgtgat atgccaatag gtatggaaca taatatagca aggattgcag gactcaaatc
    52561 tcgtaaacat atggatataa gatactttgg attaaatcac tttggttggt atacttcaat
    52621 taaagataaa aaaggaaatg aacttttacc aaaattagta gaacatataa aagaatatgg
    52681 ttttataaat ggtgaagaag gaatgaaaaa tgacaagaaa gatagttggt ttgaaactaa
    52741 tttatttact aaagaaatag taaaaacaga tttaactaca ataccaagta gttacttaaa
    52801 gtactatcta tttccagatt atgttgtaaa tcattctgat gtaaattata ctcgtgccaa
    52861 tgaagttatt gatggaagag aaaaagaagt ttttggttca tgtgcattga tagaaaagca
    52921 aggacattct aagggcagtg ggcttaagat aggaatacat gcagagttta tagtggattt
    52981 agctacagct ctagctttta atacacagga gcgtatgtta ttgatagttc aaaataatgg
    53041 agctatagaa aatatacaag atgatgctat ggttgaaatt ccatgtatag ttggaaaaga
    53101 cgggtatgaa ccattatcaa tagggaaaat accaacattc caaaaaggtt taatagaaca
    53161 acaattagca gttgaaaaat tagtagttga tgcttggatt gagaagtctt ataataaatt
    53221 gtggcaagca cttatgctat ctaagacagt tcctagtgca acagttgcaa aagaattact
    53281 agatgattta atagaagcaa ataaaaatta ttggccaaaa cttaattaag aataatatat
    53341 ttttaaagtt cctacagttt agtacatagc ctagttaatg tatttgtagg gactttattt
    53401 atttacaaat gagatacaag tggcaaacat agtgtatttt gcagttagga aagtaggttt
    53461 agttacttgt tagttggatt aaatttaggt acaataattt actatcaatc aatagaaata
    53521 accagagaag ttgcataata aaaaatttac acttttaaca ggatttatgt aggtgtttat
    53581 agaaataaat aaatatgtac ataaatacat aattattttt aagtctaaaa tacaagaatg
    53641 gagagatggc tatgaagtgg agagattatg ctgatgatgt aaatttattt gaggattttg
    53701 ataaaactat caagcctcta actgacgaac aaagaagaaa aaatataaac actttaattg
    53761 catatttttc aaaagaagtt ccttctaaaa tatacaattt atcaaatgat gaaattaagc
    53821 ctagagatat acttagagga cttttgaatg tatatccacc aaaagaaatt gctccagaga
    53881 tacttaatat gttacataat ttattgttga ttgaatgtga agaaagagag ttagtagatg
    53941 taaatgatat agaagaagta gaggaaggga ttgccatatg gagaggtaat ataacgaatt
    54001 taagagcaga tgcaatagta aatgctgcaa ataacaagtt attagggtgt ttacaaccat
    54061 tacatctatg cgtggataat gagatacatt catgcgcagg tcctagactt agagaagatt
    54121 gtgacaaaat aataaaaaaa caagggcatt tagaatatac tggagatgct aagataacta
    54181 gaggctattg tttacctgca aaatttgtag tacatactgt tggacctatt gtatctggtg
    54241 gtcagccaag taaagagcaa gaaaagcaac tgcttcattg ttataaatca tgtttaaata
    54301 caataaaaga aattgatgaa ataaaaaata ttgttttttg tggaatttct acaggtgtat
    54361 ttggttatcc aaaaaaagag gcagctaatc ttgctgtaag tagagttaga ctatggctaa
    54421 aagaaaatcc tgaaaaaaat ttaaaagttg tatttaatgt gtttactgaa gaagaagaag
    54481 agaaatatag aagaatattt aaataatatt atgtaacaga atcctaaaaa tacttattat
    54541 ataatacaaa ttcattgtat aagataataa tttacaagta gtaaaaatta ataaaatata
    54601 atttgtattt ttaataaata agtaggtata atagaaatta taagaatagt ataatcaatt
    54661 atgctattac ttagaaaata taatatagga aaattgtatg gtaaataaat atgaaaaata
    54721 gttttaaaaa ttgtattagt ttaagaaata aatttataat tatatcaatt gtgtttctat
    54781 taatcctatt aattgtatct tatatatttg gcaaattagt gtataatagt tcggttggtt
    54841 tagagcagtt aataaaaaac gaagatatag taaaaatatt ttctgagaga aaagataaac
    54901 cactggagaa attaaaaaac tacaagacat ctgaattaat gatacaaagt tctaataatt
    54961 acaagcttga aagtcttttt ataacttcaa atataaagac aagagatact atgatacttg
    55021 ttcatggaat tggaagtagt tactatgaaa tgttaaaggt agcatataga tatttagata
    55081 aaggttataa tgtattagtt tataatcaaa gaaatactgg taatagtggt ggagataatt
    55141 acacatttgg tctttatgaa aggtatgatt tagatagtct agttaaattt gtaaaaaata
    55201 aatttcctga aggaagatta ggagtgcatg gattttccat gggagcaggg acagctgcta
    55261 tgcattcaga gataaattca aaagatgata aggtcgattt ttatatactg gattctcctt
    55321 attctgaaat gaaagatgca attagaatgg gagtactaga aaaacgaata ccagatatat
    55381 taatcaatta tgtggtgatt tgtggagatt tatataataa gtttaaatct ggattttggt
    55441 acagtgatgt aaaaccatat gagtcagttg agaaatctaa tgtacccata ctgtttatac
    55501 atggaacaaa agatactgtt tgtaattatc aaaatagcaa gaaaatgtat gatttagtta
    55561 aacatgataa aaaagaccta tggcttatag aaggtattgg tcatgttgat ggatttgaac
    55621 atgatagtac agtatacttc aataaaatat ttaaatttat agatagtaat gttttaagtg
    55681 ataaaagtta attgaatatt agtagcttat ttatttttgt atttattaaa aaatagatag
    55741 gctattttta tgtttaaata taagttttgt ttagtaatgt gaattaattt ctagtaaaaa
    55801 tgaaaataac taccaattaa attcatttat acctcaaata aaagagttag tattagatta
    55861 caatcaaatc taaagaaaag agaggtagta tatatgaaaa acaaaaataa taaagataaa
    55921 aacatgttga atattaaagt attgagagtt ctaggagtgc tattaatact ttttgattat
    55981 acgggtgata ctttgaattt ttataatttc ttcgcaaagc cattagtatc gggtaattta
    56041 tataagacaa caggtactga ctgtattgta tttttattat ttattgtaga gtgtgtatac
    56101 ctagtgggaa gatatatatt aaaagttaag ataaattgga gtgagattat tgaaaatgag
    56161 aaaggtaatt ttataaataa cttgttggtt gaaaaaatac aagaagaaag caacattgta
    56221 tataatttat taaactcata tgtgttgcca catatatata agaaagcaag ggtgttgatg
    56281 tttaaaacat atcatatgtt aatctacatt aaagaaaaat actattatgt aaatatatat
    56341 taatataata tgattgcaca attaattata ttatattttg tattggatga taaaatgaaa
    56401 gacataaatt ttttgagaca ctaaaatcgc aatttatttc gtgtttaagt tataaatttt
    56461 gtgaaaaatt ataaatctta taaaatagat tgcgatttta atcttaaact tttgctatag
    56521 tttttccagt atcagttaaa tcatatccgc caagaccttg aagttcagct ttaaactcag
    56581 gagagtttat tatgttaatt atagtttggt aaactggatt ttgtaagtct tcttttttaa
    56641 taactaaatc atagcgctct ttgtgaagag gaataaagtc gatattattt acttgtaaag
    56701 cagctttttc atttcccaaa ccaacatcag ctattccacg agaaacagta cttgcaactc
    56761 caagatgtga taattcttca ttatcataac cttttatatg tttaccatta aagtttagaa
    56821 gtctaagttt accatctagt aaaaccctag ttccagaacc tttttcccta tttacaatgg
    56881 ttatacttgg ttttgttaaa tcctcccagg ttgttatctc tttaggattt cctttagcaa
    56941 catagaaacc ttgcattcta taagctagat ttataagtac acatggtatt cctggcaata
    57001 gctttttaac aaaagtgcta ttatattcat ccgtttcgaa atcccataga tgggatgatg
    57061 ctatagaaac tctgccatta tataactcat acaaagcatt ataactacct atataagagc
    57121 gaaatgtact tattccatcc aatttttttt caacagacct agctaaaatg tctaatatta
    57181 catcttgacc agaaataata attttgaaat tattatttat atctatattt ttattgttga
    57241 aatgtgtatc ctttacttta ccagtttttt ggctgttgat atattcttca acatctttag
    57301 tatcaactct tatttttttc cctactttat aagaaggaag ttcacctctt ttgattaatt
    57361 catatacagt attttttgtt atctttaata attcagctac ctcgacaggt gtcaaagatg
    57421 ataatgagtc cataatccac ctccatataa ggttatttta acattaaaaa aaggaaaatg
    57481 tagcaaaata tttaaaagta gtgtaaataa attacttgaa aatttattat catgtatata
    57541 taattaaact atatataaca aaacatgata aaacataata aaacatatta aaatgtgtat
    57601 caaatttaca aaaagggaga ttgttatgaa aaaaatattg ggaatattag gattagttgc
    57661 atgtttaact ttggggacag ttgggtgcaa tagtaatgaa gacaaaacag atgatggaaa
    57721 acaagaaaaa acaactaaaa gtagtgattc agtagagtta aatatatcgg ctgctgcaag
    57781 tttaaaagaa gcaatggcaa agattgaaga agaatataaa aaagttgatt caaatgtaaa
    57841 acttacagtt aactatggag catcaggctc attacaacaa caaatagaac aaggagcacc
    57901 ttgtgattta tttatttcag caggtcaaaa acaaatgaaa gctttggatg aagagaagtt
    57961 attagtttct gatactatga aagatttagt aaaaaatgat ttagttctta tttcttctgc
    58021 tgacagtagt gtatcaggta tgaaagattt gactacagat aaagtgaaga aaattgctgt
    58081 tggtgaagtt gaaagtgttc cagctggtaa gtatgcagat gaggtgttaa ctaatttaaa
    58141 tttaaaagat aagttaaaag ataagctagt atttgcaaaa gatgtaaaag aagttttagc
    58201 atgggtgcaa tcaggaaatg ctgatgttgg attcgtttac ttcagtgata ctgtcaataa
    58261 tgataagata aaagtagttg aaaaaacaga cgaaaaaact cattcaccta ttacatatcc
    58321 agtttcagta attaaagcta gtaaaaacgt agatgctgct aagaaatttg aagaattttt
    58381 attaagtgag tcaggacaaa agatttttga agaatttgga tataaaaaag tagaataaac
    58441 ttataaataa attatgtgta attaagtata aattatatat aaataaatga aaattgaaaa
    58501 taattgtgta ttataataaa atggaaatag tgaaattaat agagttatta gtttcactat
    58561 ttccatttaa aattaaggag ggaaagatgg gaacagattg gtcaccctta tggatatcac
    58621 ttaagacatc tattttgtca acaataataa cttttgtaat aggaatattt gtgtcatata
    58681 ttatggctaa ctatagaggt aagtggaagg gtcttataga tggactattt acacttccac
    58741 taattttgcc accaacagtt gtaggtttct ttttattatt actatgtggg aagaatggat
    58801 ttataggaaa gtttttatta aatttcgata ggactttaat atttagttgg acagcaactg
    58861 taatttcagc agtagtggtg tcttttccta tgatgtatag aacttcaagg tcagcttttg
    58921 aacagattga taataatatg ctgtcagctg ctagaacatt aggtcttaat gaatggaaag
    58981 tattttttaa gattgcagtt ccattagcat ggcctggaat aattggtgga ttggtgctgt
    59041 cgtttgccag agctcttggc gaatttggtg ctacacttat gatagctgga aatatacctg
    59101 gaaaaactca aactatgcca atagctatat tttttgctgt tgaaggcgga gatatgaata
    59161 aagctatgat gtgggttatg attattgtag caatatctat agcaatgatt ttattactaa
    59221 attattggtc ggaatttcag caaaaaatta taggaaagag gtgtggatag ttgagcttat
    59281 acgtggatat tgaaaaagat ttatcctctt ttaaactaaa ggtagaaatt aaacaagaaa
    59341 aaggtactct ggggtttttg ggggaatctg gttctggaaa aagtatgaca ttaaaatgta
    59401 tagcagggct tgagaaacca acaagaggta aaattgtatt aaatgataga gttctatttg
    59461 attctgaaaa gaagataaat ttatcaacac aagatagaaa ggtaggattt ttatttcaaa
    59521 actatgcttt gtttccacat atgactgtaa gtcaaaatat agaacttggg cttttaaaac
    59581 taagtaagag tgaaaaaaag gaaatagtag caaggtactt agatatactt aaattaaatg
    59641 ggtttgaagg gagatatcct tggcaattat ctggtggtca acaacaaagg gtagcactag
    59701 ctagagcatt ggcaacatct ccagatatat tacttttaga tgaacctttc tcagcactgg
    59761 accatcattt aagaagtaat atggaaaaag aacttatgaa tatgttaaaa gattacaagg
    59821 gagatatact atttgttact catgatattg aagaagctta ccgagtttgt gatgatatta
    59881 tagtgtacaa caaaggagaa ggtcttccaa agagacctaa aaaagagctc tttgaatctc
    59941 caaagtacct tatagaagct aagataacag ggtgtaaaaa tatatccaaa ttaaatagac
    60001 tggataaaaa tactatttat gctacagact ggggatgcga gttgacatta aatagagaaa
    60061 taggtgataa tatagaatat gtgggaatca gggagcatca tattaaggtc ttggattcta
    60121 atgaagattt aaatgaaaag ttatgtttcg aattgattaa cattgtagaa aatcctttta
    60181 catatactat atatgttaga aaaactgatt tatctaacga atgtgtacct attcaaatag
    60241 aattggaaaa aagtaaaatg agatttaaga aaggtgacag aatatattta gattttccac
    60301 aggaatattt attttgcttt agatacaatt ataacaaaaa ggagtaaact atatgactga
    60361 aaaagaaaaa atgttgtctg gaaaaggata ctatgcaaat gatgaattgt tagtaaaaga
    60421 aagagagtat tgtaaaaaat taactagatt gtttaataat actttagagg atgaatatga
    60481 aaaaagagaa gatattttaa gacaactatt tggttcagtt ggcaagcaaa taaatgtaga
    60541 acaaaacatt aggtgtgatt atggatataa catacatgta ggcgaaaatt tctttgcaaa
    60601 ttatgattgt atttttttag atgtatgtaa aattgaaata ggtgacaatg taatgttagc
    60661 tccaaacgtt caaatctata cagcctatca tccaattgat gcacaactta gaaattcagg
    60721 catagagtat ggttctcctg taaaaatagg tgataatgta tggataggtg ggggagtaat
    60781 cataacacca ggaattacta taggtgataa tgtagtaata ggtgcaggaa gtgtagtcac
    60841 aaaagatata ccaccaaata ctgtagctgt tggaaatcca tgtagagtaa taaagaaaat
    60901 agaagaataa atatgattgt ataaagttta tacaagtaaa atttatacaa tcatattaag
    60961 agcatttaca tataatcttt aaatagataa tacattaaaa taaaaatata tgcctgtttc
    61021 tataaattaa tttatagaaa caggcat
//
LOCUS       DAMKPG010000272        35480 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.033, whole genome shotgun sequence.
ACCESSION   DAMKPG010000272 DAMKPG010000000
VERSION     DAMKPG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.033"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            247..864
                     /locus_tag="PC621_003890"
     CDS             247..864
                     /locus_tag="PC621_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF4110948.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            972..3503
                     /locus_tag="PC621_003891"
     CDS             972..3503
                     /locus_tag="PC621_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF4110949.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3937..4638
                     /gene="vanR"
                     /locus_tag="PC621_003892"
     CDS             3937..4638
                     /gene="vanR"
                     /locus_tag="PC621_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF4110950.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKAQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4688..5770
                     /locus_tag="PC621_003893"
     CDS             4688..5770
                     /locus_tag="PC621_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF4110951.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6031..7131
                     /gene="vanG"
                     /locus_tag="PC621_003894"
     CDS             6031..7131
                     /gene="vanG"
                     /locus_tag="PC621_003894"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF4110952.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7128..7934
                     /locus_tag="PC621_003895"
     CDS             7128..7934
                     /locus_tag="PC621_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF4110953.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7952..10090
                     /gene="vanT"
                     /locus_tag="PC621_003896"
     CDS             7952..10090
                     /gene="vanT"
                     /locus_tag="PC621_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF4110954.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10404..11024
                     /locus_tag="PC621_003897"
     CDS             10404..11024
                     /locus_tag="PC621_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF4110955.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11190..11726)
                     /locus_tag="PC621_003898"
     CDS             complement(11190..11726)
                     /locus_tag="PC621_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF4110956.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11839..12513)
                     /locus_tag="PC621_003899"
     CDS             complement(11839..12513)
                     /locus_tag="PC621_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF4110957.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12716..14590
                     /gene="asnB"
                     /locus_tag="PC621_003900"
     CDS             12716..14590
                     /gene="asnB"
                     /locus_tag="PC621_003900"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF4110958.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14682..16118)
                     /locus_tag="PC621_003901"
     CDS             complement(14682..16118)
                     /locus_tag="PC621_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF4110959.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            16447..17025
                     /locus_tag="PC621_003902"
     CDS             16447..17025
                     /locus_tag="PC621_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4110960.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            17064..17642
                     /locus_tag="PC621_003903"
     CDS             17064..17642
                     /locus_tag="PC621_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4110961.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            17728..18357
                     /locus_tag="PC621_003904"
     CDS             17728..18357
                     /locus_tag="PC621_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF4110962.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            18347..21106
                     /locus_tag="PC621_003905"
     CDS             18347..21106
                     /locus_tag="PC621_003905"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF4110963.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            21193..22755
                     /locus_tag="PC621_003906"
     CDS             21193..22755
                     /locus_tag="PC621_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF4110964.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            22756..23892
                     /locus_tag="PC621_003907"
     CDS             22756..23892
                     /locus_tag="PC621_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF4110965.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            23956..25032
                     /locus_tag="PC621_003908"
     CDS             23956..25032
                     /locus_tag="PC621_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF4110966.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            25010..26248
                     /locus_tag="PC621_003909"
     CDS             25010..26248
                     /locus_tag="PC621_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF4110967.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            26302..27390
                     /locus_tag="PC621_003910"
     CDS             26302..27390
                     /locus_tag="PC621_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF4110968.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            27418..28209
                     /locus_tag="PC621_003911"
     CDS             27418..28209
                     /locus_tag="PC621_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF4110969.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(28324..28878)
                     /locus_tag="PC621_003912"
     CDS             complement(28324..28878)
                     /locus_tag="PC621_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF4110970.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            29073..30275
                     /locus_tag="PC621_003913"
     CDS             29073..30275
                     /locus_tag="PC621_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF4110971.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            30332..31312
                     /locus_tag="PC621_003914"
     CDS             30332..31312
                     /locus_tag="PC621_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF4110972.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            31559..32512
                     /locus_tag="PC621_003915"
     CDS             31559..32512
                     /locus_tag="PC621_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF4110973.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            32502..33458
                     /locus_tag="PC621_003916"
     CDS             32502..33458
                     /locus_tag="PC621_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF4110974.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            33455..34210
                     /locus_tag="PC621_003917"
     CDS             33455..34210
                     /locus_tag="PC621_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF4110975.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            34302..35267
                     /locus_tag="PC621_003918"
     CDS             34302..35267
                     /locus_tag="PC621_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF4110976.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        13817 a         3710 c         6140 g        11813 t
ORIGIN      
        1 tcaaaatgaa ctttttagtt catgaggcac tctttttgta cgaaataaaa tatattttta
       61 ttatttcaac aatgaaaaag atgcttgttt ctattttgag acagtataat ttttatgtaa
      121 ctttaatttt agtaaatata aagttttaaa attgataaaa taggaggaat ttttaatata
      181 aaagggtaaa taaagggttt agagggcata atatagaata aattaatata tttaaggagg
      241 atttttatgt gtaaaaaaac gtatttatta gtggtaagta tacttatagc aggtttatta
      301 actgcatgta gtgggagccc aactaaggat tcaggacaag cacaagataa tacaaaaaag
      361 gaggcaaatg cttcaaataa tgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa
      421 caagatttta aagtgccata cacagatgca ataaacatat ttaaagataa atacaaggat
      481 gctgatatag tagatttaag tcttgagaga gatttaaata aatttgttta tactgtggaa
      541 ggtgtagatg ataataatga atataagatg aaaattgatg ctaatacaaa agatgtatta
      601 gaagataaaa cagaaaagtt agattcagaa gatttaaatg gagttgctag aaaagaaaaa
      661 ttagatttaa atgacataat tacacctcaa caagctatgg aaattgcatt aaaagaacaa
      721 aatggaatgg ttaaagaatg gagtttagac aaagatttag atgttacttt ttataaaata
      781 agaatagata aagataaaaa tgaatatgac ataaaagtag attctaaaaa gggaactgta
      841 ttgaaggtag aaaaagaaga ttagaagatt taaactatct ttaaaataat ttcttttttg
      901 aataaatttt gtttaaaata taacaagcag ggtataaaga gactaaataa ctattttagg
      961 aggagtacgg tatgtcattg aaatcactaa aaataaaaga aaatttatat tgggtaggtt
     1021 cacttgaccc tgatttaaga gtatttgaca taattatgta tacaccttat ggaactactt
     1081 ataattcata tgtattaaaa ggaacagaaa aaacagtttt gtttgagaca gttaaagata
     1141 agcattttga caattatatt gaaagattaa atgatttaaa tatagatttt gaaaaaatag
     1201 actatattgt tgtaagtcat actgaaccag accatgcagg aagcgttgaa aagcttttgg
     1261 atttagctaa aaatgcaaaa gtagtagctt cagaaacagc aattaaatat cttaaggaaa
     1321 tcgtaaacaa agactttgaa tatgttgcag ttacagatgg agatacatta tcaattgggg
     1381 acaaaacttt agaatttttc tctgtaccta tgcttcattg gccagatact atatacacat
     1441 atataaaaga ggataaaact cttgtaacat gtgattcatt tggtagtcac tatagtaatg
     1501 ataagatagt aaatacactt gatgaaaatg aagaaaagga ttacttggat gctttaagat
     1561 actattatga ttgtataatg ggtccattta aaccatctat ggtaactgct atagaaaaga
     1621 taaaagattt agatatagat actgtatgtc caggtcatgg tccagtatta actgaaaatc
     1681 ctagaaaaat tatagacctt tattataact ggagtgtaaa ggaacaaata aagttagaaa
     1741 aagaagttac aatttgttat gtttctgcac atggatatac aaagattatg gcagaagcta
     1801 taaaagcata tattgaaaaa aatagcaact ataaagtaaa tttatttgat gtaatagagc
     1861 ataagcaaga agatattttg gcaaaaatag cagtatcaca aggtgtatta tttggaacac
     1921 caacaatatt aggagatgcg ttaaagccta tatgggatat attaatatcg ttaaacccag
     1981 ttcttcatgg tggaaaagtt gcttcagtat ttggttcata tggatggagt ggagaaggta
     2041 tagaaaatgc tatggagaga ataagtcagc ttagaatgac agcagtaaaa ccttttgctg
     2101 ttaactttaa accatcaaat gaagagattg ataaattaca ttcttataca ggtaaattcc
     2161 tagataagtt aaattctact tttggaagta aaaagaaaac taagaaattt aaatgtgtaa
     2221 tttgtaatga agtttttgaa ggtgatagtg ctcctagtgt atgccctgta tgtggagcta
     2281 aggaagacca atttatagaa gttgaagaag atgaagttac ttttagaaaa gatactgatg
     2341 aatatttcgt aatagtagga aatggagcag ctggttttta tgcagcagat gctataagaa
     2401 aaagaaataa aacttgtaag ataactatga tttctaatga agatgagttg acttactata
     2461 gacctgcact ttctgatggt ataaatgaag aacttggttc agatttctac atggaagata
     2521 aagattggta tgataaaaat aatatagtag taatattagg aacgaatgta gataaattag
     2581 atgaagtgaa taaaactatt atagtgaatg atggagctat taaatttgat aagcttgtaa
     2641 tagctacagg aagtagaaac tttataccac cgattaaggg ccatgattta gaaaatgtgt
     2701 ttactcttag aaatataaaa gatttatata gtgtaaaaga agcactagaa aaatctaaga
     2761 aagttgtagt gattggtggc ggtttattgg gacttgaagc tgcatgggaa tttagactaa
     2821 aaggattaga agtagtagta gttgaagcca tggatagcat actatcaaaa caacttgata
     2881 aagagggtag taaaatatta gaacaatgtg taagagatac aggaatagat gtaagattgg
     2941 gtgtagcagt tgatggtatt gaaggtgatg gaaaagctca aaaagttgta tttaaagatg
     3001 gagatagtgt tgactgtgat atggtagtat tttcaattgg tgtaagagca aacactcaaa
     3061 tggttcagga tacatctgta aaaattgata gaggtattgt tgtagataag acattacaga
     3121 caaatgttaa agatatatat gcttgtggag atgttgcaca agtgggaaat attagtttag
     3181 caatatggcc atcttcagta gaaatgggaa aaatagcagg agcaaatgca agtggagata
     3241 atttgacatt tgaaagtgaa gtatatccag tttcattaga tgcaatgaat gtaaaagtat
     3301 ttagtattgg taatatacaa aattttgata aagaaatttc atcaaaagat gaaggtcaaa
     3361 gaatatataa aaaattattt atgaaagatg gttcattagt tggagctata cttataaatg
     3421 atttaagctg tactgttaaa ttgataagat taatatctga aaaaggtgat tttgaggata
     3481 ttatgaaggc tgatatacta taatttaaat acattaaaat aaaaaggagt tgtctcaaaa
     3541 ttgatttttt tctaatcaaa ttttaaattc aactcctttt ttgaattttt atataaaata
     3601 acatggaaat tttagtaatg tctgtggtta catacatagg tttaattgtt ttttatatgg
     3661 attgtctctg gtataataaa ttgaattgtg tttgactgat agatattttt attgaaagtt
     3721 aatacctgtt atgcgataat aaggacgtgt taggggagtt tttataataa caattaatct
     3781 cagtttttgt gttccaatag tagcatcaac acattctgat gtagtagttc ttttcttata
     3841 aaaattagtt acaaatatgc ttgtaattat ttgatatgta aatttaccaa tattataaat
     3901 gatgacctcg ataataagga gagtgaaaaa taatctatga acgaaaatat tttgattgtg
     3961 gatgatgaaa aagaaatagc agatttaatt gaagtatatt taaaaaatga aggttttaca
     4021 gtatacaagt tttatacagg taaggaagct ctagaatgta ttgaatctaa agatttggat
     4081 atggctattt tagatattat gcttcctgat attgatggat ttcatatttg ccagaaaatt
     4141 cgagaaaaat actattatcc tatcattatg ttaacagcta aaattgaaga tatagacaag
     4201 ataatgggtt taaccatagg tgctgatgat tatataacaa agccatttaa ccctttggaa
     4261 gttgttgcaa gagtaaaggc acaattaaga agatatatgc gatataataa ttcttatgaa
     4321 caacagtcga taattgttaa tgagtatgat attaaaggat tgattatcaa taaagaaaca
     4381 cataaatgta gtttatttgg gaaagaagta gcattaacac cgattgaatt ttctattctt
     4441 tggtatttat gtgaacatca aggaaaagtg gttccttctg aagaattgtt tgaagctgta
     4501 tggggagaaa aatatcttga taataataac acagttatgg ctcatatagg gcgattacgt
     4561 gaaaaaattc atgaaccttc taaaaacccg aaatttataa aaactgtatg gggagtaggt
     4621 tatacaattg aaaagtaaaa atattaaagt caatcctgac tttactcaag tgagaaataa
     4681 aatttttatg agaacagctt taatggcatt tatagccatt attttcatat ggattttata
     4741 ttcttttata ttgtatggta agttttctat ctggatagta tcattctttg aaaatgcttt
     4801 tcgtatggaa tataaagctg cattgaattt atatcagcag attttccgta atagcatgga
     4861 gctcttcatg atactagcta tggtgattgt gttttttata atattccgtg tttatttaaa
     4921 ttggtttaca aaatatttta gtgaaataaa tcaaggtatt gactctctta tcaaagaaga
     4981 tgttggagaa gtggctttat ctccagagtt attggcaata gaaaagaaaa taaactcaat
     5041 taaacatata cttgaacaac gtaaatttga gacacaaatg gcagaacaac gtaagaatga
     5101 acttattgtt tatcttgcac atgatttaaa aacaccactt acatcagtta tagggtatct
     5161 gacactactt cgagatgaaa gtcagatttc tgaagaactg cgaaaaaaat atttatctat
     5221 ttcgttggat aaggcagagc gtcttgaaga tttaattaat gaattctttg aaattacaag
     5281 gtttaattta tcaaatatat cacttgaata cagtacagta agcttaactc gtatgcttga
     5341 acaactaaca tatgagttta aacctacttt gatgagtaag aatttaaaat atacactgaa
     5401 tgttacacca aatatgatga taaggtgtga tgttaataaa atgcaacgtg tatttgataa
     5461 cttattacga aatgctgttt attatagttt tgaagatact actattgaaa taacagctgt
     5521 acaggaggat gattatataa agcttaaatt tataaatcat agcaatacga ttccagagga
     5581 aaaacttgag cgtatttttg aacaatttta caggttggat acatctcgta attcaaacag
     5641 tggtagagct gggttgggac ttgctatagc taaagaaata gtggagcttc ataacgggac
     5701 aataacagct catagcgaga acaatataat tacctttaag gttattattc cgattgtagg
     5761 aaatttgtaa gaatttaagt ataaaaaaat aggaaattct ttaggataaa taaataaaga
     5821 gcactcttgt tattgatata attactgtat caaaacaaga gtgcttttat tttgatacag
     5881 taattatatc aatagaaaaa tgtgaaaata tattatgtgg aacagatgca attgtaagtg
     5941 aaggttgatt ggatttgaaa tagaaaaagt attttaacat gtttctgtag attgtataaa
     6001 aataaataaa tcaacaaagg agaatcaaag atgaataata aaaaaattgc agttatattt
     6061 ggtggaaact caacagaata tgatgtatca ttacaatcag cttttgcagt acttgagaat
     6121 ttgaatacgg aaaaatatga tattatacca attggaatta caagggaggg taattggtat
     6181 cattatattg gagattataa taatatccag aataacactt ggtttgaaag ggatactgat
     6241 ataattccag ttattgtttc gcagaaccgt gtcaaaaatg gtattattga atttttaatt
     6301 cctgatgctg tttctcagaa tcatattcca aatagtgcta ttgaatcatg tgaaaatgat
     6361 attaaataca ctaaacttga ttttgtattt cctgtattgc atggaaaaaa tggagaagat
     6421 ggaacagtgc aggggttatt tgaattggca ggtatacagg ttattggatg tggtactctt
     6481 tcttcagcat tatgtatgga caaagacaaa tcacataaac ttgttcattc agaaggtatt
     6541 aaagtaccaa aatcaatatt actaaaatac tcagataaat ttaatgtggc tgaactgtca
     6601 aaagacttaa cttatccatt atttataaaa cctgtgcgtg caggttcatc atttggtatt
     6661 agtaaaattt ataaaaaaga agagttacaa gtagcagtag acttggcttt tgaacatgat
     6721 aatgaagtaa taattgagga aaatattgat ggttttgagg tagggtgtgc agttttagga
     6781 aacgatgact tacttgttgg aagagtagat gaaatagaat tgtcaaaagg attttttgat
     6841 tatacagaaa aatatacatt gaaaagttca aaaattcata tgccagctag aattgatttg
     6901 gattgtgaga aaagaataca agagacagca aaaattattt atagaactct tggatgttct
     6961 ggatttgcta gagtagatat gtttttaact ccatctggtg aaattatttt taatgaagtc
     7021 aatacaattc ctggctttac ttctcatagt cgttatccga atatgatgaa aggtattgga
     7081 ctgtcttttg aagaaatatt ggataagata attgaggtgt acataaaatg atgaataata
     7141 ttttattaga taaaaataaa gaagataaaa tgacaaatac tatcttattg actaaagata
     7201 aagtttatga tgggaatcta attctagtaa atgctttttt accagttaaa acatctgaag
     7261 atatagattt aattcctgta gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta
     7321 caaatatttt acaaaatatt ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa
     7381 gtggatatcg tactgcagag gaacaacaag atatatattc aagctctctg cgtgacaatg
     7441 gaaaagactt tactaaaaag tttgtagcac ttccaaatca cagtgaacat cagacagggc
     7501 ttgctattga tttagggttg aagaaagagg ttatagattt tatctgccct gaatttcctt
     7561 ataatggtat ttgtaatgag ttccgtaagg cagcactaca ttatggtttt atagagcgtt
     7621 atcagtgtaa aaaagaagaa ataacaggaa tatctcaaga accatggcat tttcgttatg
     7681 tcggatatcc acattctgag attatcaata aaatgaatct cgcccttgaa gaatatatag
     7741 aatatattaa gagattttcg tatacaaatt gtttaaaaat agaagataaa gaaaaaatta
     7801 tagaaatctt ttatgtgcct attcatacta aagaagaagt gagaatctct atacctgaac
     7861 aatgtgtata tcaaatatcg ggtaacaata aggatggttt tattgtgaca ttttggagaa
     7921 atagaaagga ctaagaaatg gaggaacaag aatgattagg aataaatctt atgcagggct
     7981 tgattatttt aggattatag ctgcattact tataattgca attcatacat ctccattatt
     8041 atcatatagt aaaatggctg actttgtgtt aactagtata attgctcgta ttgctgtgcc
     8101 attctttttt atgctatcag gcttttttct aatttcaaca tataattata attgtaataa
     8161 actgaaatct tttctaaaaa atgtattatt tatttacggg atagctattg taatttatat
     8221 tcccttaaat atttacactg gatatttttc catgaataat ttagtgccca atattattaa
     8281 ggacattgta tttgatggga ctttatatca tttatggtat ctacctgcat caattattgg
     8341 tgcaagtatt gcatactttc ttgtaaaaaa actaggttta aagtactcat tagtaatcac
     8401 tattgttatt tatgtaattg gtatgtttgg tgatagttat tatggttttt tagaaaaaat
     8461 accactatta aagaatttat atgggtatat ttttgaagta tcagattata cacgaaatgg
     8521 tatatttttt gcacctgtat ttttcatact tggtggaata atatctaata aatctaatta
     8581 tatgtcaact aaaaatagct tgattggatt tcttatatca ttcttactta tgttatgtga
     8641 gggaatgttt ttacataaac taggggtaca gcgtcatgat agcatgtata ttatgttatt
     8701 gccatgtatg tatttcttat ttacagcctt aacattttta aaaggttctc gagctaggtt
     8761 attgcgaaca tcatcattaa ttatttatat tatacatcct atgatgattg ttgttattcg
     8821 tatgttttct aaaatattcg gaatacaaac tgtcttggtt gacaatagcc ttattcatta
     8881 tatagtggta agtatagcat cttttatagc ttctataatt attatattga ttcttaaacc
     8941 tctaaaaaaa gacttgaaag gagaacataa ggtaaatacc aatagagctt ggattgaaat
     9001 tgatttaaat aatttggaac ataatgtaaa gatgatacaa aaagctatgc cagaagattg
     9061 tgaattgatg gcagttgtaa aagctaatgc ctatggtcat agtgcttttg aagtagcgac
     9121 ttgtgcaaac caaataggtg taaaagcatt tgcagtagca acaatagatg aggggattga
     9181 tttacgtcga tatggaatac taggagaaat attgattctt ggatatacta acccaataag
     9241 ggcaaaggaa cttcataaat acaatcttac acagagtgtt attgacctta atcatgctat
     9301 ctctttaaac aaacagaaat acaacataaa atcacacatt aaaattgata caggtatgca
     9361 cagactgggt tttgatgtta atgatgtgag gtcaatttta aaggcattta aattaaaata
     9421 cttagatata tgtggcatat ttacacactt gtgtgtttca gatagtctga aagatgaaga
     9481 tgtagacttt acaaataaac aaattgaatg tttttataag ttaattgatt ttctgttaaa
     9541 aaaaggtata aaaattccta aagtacatat tcaaagtagt tatggttttt ttaattatcc
     9601 caatcttaag tgtaattatg taagaattgg aattgcatta tacgggatat taagttctcc
     9661 aaatgatacg actaaattgc aacttgattt acgacctgtt ttatccttaa aatcacaagt
     9721 aattcttatt agaaaaatac aaaaagatga tagttttgga tatggaagag tctttattgc
     9781 aagcagagat agcgtaattg ctatattatc tattggctat gccgatggta tacctagaaa
     9841 tttatcctgt ggaaaaagtt acgtaataat aaatggttgt agagcagcaa tagtgggtcg
     9901 aatttgcatg gaccaacttg ctgttgatat tacagatata cctaatgtag aagttggtaa
     9961 tacggcaata ataataggaa gagataattt atctgaatta tctgcttctg aagttgcaaa
    10021 taactcatgt agtatatcga atgaattatt gagcagagta ggaagaaggt taaatgttat
    10081 aaagaagtaa ctcaattaaa tagtaatatt tgttgtactg tgtaatttat tttactctga
    10141 ctagacattt ttagtaatat tatgtctaat atatattttt tataacttac atgacaattt
    10201 aagttataaa aaggtgatgt ttcagatttt tattttgaaa catcaccttt tttgttataa
    10261 tatataataa aataattata ccttaactgt aaacttatat agattattta ctttttaata
    10321 ttagattcat tttttgagat tggtatataa taaacatata gagtatataa taatagttaa
    10381 agttaaaaaa ggggaaaagc aatatgcagt atatacaaga attacttgat ttgactcaaa
    10441 attattgggt gttagccaca ataatcggct tattaagtgc atttatagaa agctttatac
    10501 cagccttacc acttgttgct atagttacag caaatgctgc aatacaagga ttatttattg
    10561 gatgtttgtt atcatggatt ggctcaggac ttgggacaac ttcattattc ttattaataa
    10621 gcagatttaa tgatagtaag ctttttaata agttgagaaa tagtaaaact gaaaaagcaa
    10681 taagttggat ggataaacag ggatttaagt tattatttat agcgtatgcc tgtccattta
    10741 tgcctggttg tttagttact atcgcatcag cttttggtaa aaaagattta aaagattttg
    10801 tgccagcaat gttggcagga aaatttgtaa tgtttatagt tataagttac gtggcaagtg
    10861 atatagaagg ttttataaca agtcctttaa agatagcatc atttatatta ttggtgtttt
    10921 tgtcatggaa gattggaaat agagtaaata aaaatcttga aaatcataat tatgatttcc
    10981 atcataaaaa acatcacaat gataaagatg ataaaatgat ttagcttgta cgaaataaaa
    11041 cgtatcttaa aatgatttag tactaaatta ttttaagata cgttttattt tttgtattaa
    11101 ggtacaaaat ttttagttct tttgtaaact aaaatatata aaacttattt atacttagtt
    11161 caaaatattg ttatttattt tttctacaac tataataaaa tataaaattg atatccaatc
    11221 aaaaaaataa ctatgaaatt taatagctta gatatgaatt tttctgtttt tgagttagtt
    11281 tttataggta gtaaagaaat gtttatggta atgggataaa acaattcaat accttcttta
    11341 gtaataaggt caagacacaa gtgagataga tatcctatta taaacccact ggacaaacat
    11401 gagaaaacta tattattgtt agtgaagatt gtcagagatt taaaaaaact ataaataagt
    11461 aataaggcaa ttagactgtg agtaaaccct ctatgtctaa acctacttcc aaataatttg
    11521 tatattaaca taaatttttt acttatgtaa gaacctctca tatctatatc tggaaataaa
    11581 gaacctaaat atgcaaaata aatataaatt ataattaata ctattttata tgcaatatcg
    11641 tatttaacta ggtacagatt gtttataaag gggagtatca aaagtgcgaa tatataacct
    11701 cccctttcat gtgtttcttt tatcatataa aaatcctcct gaatcgtcac tataaacatt
    11761 atattggtaa atttagacta tcttttatta tattatatag caaacataag tttttttcaa
    11821 aaggatttta atatttaatt aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc
    11881 attccaattt actacattaa accagttgtc aatgtattca tttcttctat tttgatattt
    11941 taaatagtaa gcatgctccc aaacatcaag tcctattata ggagttaggt ttttacttac
    12001 agggctatcc tgatttggag tagtcataat agataatttc ccatctttag tagctacaag
    12061 ccaagcccaa ccagaaccaa agacatctaa agcagatttt tggaactctt gcttaaattt
    12121 ttcaaaagaa ccaaagtctc tatcaatagc ttcttttaaa gattcagaag gtatggtttt
    12181 ttctggcgtc attatatcaa aaaagaattt atgattataa gctccacctg cattatttct
    12241 tacagttgta gcaatatctt taggtaaaga atctaaattt tgcaataatt cacataaaga
    12301 ataattataa agctcaggat atttttcaag agcagcattt aatttatcaa cataagcttg
    12361 ataatgctta tcatgatgca gtttcattgt ttctttatct atataaggtt caagtgcatc
    12421 atatgcataa ggtaatggtt taaccttaaa tttgttattt tcaggtgtaa aagcaaatga
    12481 agttatgcac tgtgagatta taaataaaga cataataact ggtattaata tttttttctt
    12541 cataaaaatc acctcctaat atagtatttc tagaaataaa aaaaatatga aatattaaca
    12601 gataatttat aagaatttaa tagcaatttc attttaaatt gaataagttt taaagccaaa
    12661 gacaaaacta tgaaatatag aataagacta aaggacagaa aggaattttg attatatgga
    12721 cggcaatatt tcaatttatt ataaaggtga tatatctaaa aaagaagata ttaaatttaa
    12781 aataaaagaa tctgtaaata atacaagact ttctagtaga atagagtata aggatgagga
    12841 aaaaactttt gattttgaaa gatttaatgt aattgatgct atttatggaa ataaaccatt
    12901 tgaaaaagat ggaaaagtta ttgtttttaa tggaaatata tataactatc atgaaataaa
    12961 agaagaatta attaaaaaag gacacaattt tactacaaat gatgatattg aagtcttatt
    13021 ggcatcttat atagaatttg gtaagaattg tgtacataag attaaaggaa tgttcaactt
    13081 tataatctat gatagagaaa atgaaagtat atttggagca agagatttat ttgggataaa
    13141 accactttat tatataaata aggaaaatgc aataattttt tcctcagagt ataagttttt
    13201 acttgaatat atgaaaaatt taaatataaa tgaaagaagc ttacaaagtt atttttcatt
    13261 tcaatatgta cttcctgaag atactatgat acaaggaata aggctgatac cagcaggtca
    13321 ctattttagg gtagaaaatg gtatactatc tttaaagaga tataataaac ttgagtttag
    13381 aagtagtaca aaattttttt atactaaaaa tcacttaggc aatagagatg tgaatgagga
    13441 tgatataaga aatatagtag tagattcaat agtaactcat atggaagagg agaaagaaat
    13501 tggaacattt ttgtctggtg gaatagattc atctattata actactgtgg cttcacaaat
    13561 taatccaaat ataaaatctt tttctgcagg ctttagtgtc aaaggatata gtgaattaga
    13621 agtagcaaag aaaacagctg ataagttagg tatagaaaat atccagatta atataacaca
    13681 agatgaatat ataaaatcat taccgaatgt aatttattct ttggatgacc ctatagcaga
    13741 cccttcagaa gtaggtcttt atttcttaat taaagaggca ggtaaacatg ttaaagtagc
    13801 attgtctgga gaaggagcag atgaattatt tggaggatat aatatatata aagagtatag
    13861 tactatgaaa tcagttgtca attcacctac ttatattaaa agtatattag gaagagtttc
    13921 agaacttatg ccaaacataa aaggaagaaa ttatctttat agagctacaa caccattaga
    13981 aaaaagatat ataggaaatg caaaagtttt tgaaaacagc gaagtaaaaa gattcttttt
    14041 taagtataag gaaaagaata tatatgaata cctgttgtct aatttgtata gagatgcaca
    14101 aaaaaataat tacgattata taagtaaaat gcaacatata gatgtgaata catggcttca
    14161 aggcgatata cttcaaaaaa ttagcaaact ttctacagct gagcaggttg aacttagagt
    14221 accattttta tataaagatg tatttgatgt agcaaaaaat ttaagaatgg agcaaaaaat
    14281 aaataagaat aatacaaaag ttttacttag agaggcgttt agagaaatag tcccagaata
    14341 tgttgttcaa aggaaaaaat taggatttcc aactccaata agagtttggt taaaagattc
    14401 tctaggggat attgtaaagg aaactattta taattcaaat gtagatgaat ttatagataa
    14461 aaaatatgca ataaaacttt tagatacgca tcttaaaggt catagagata attcgagaaa
    14521 aatatggact atatttacat tttgtctatg gcatcaatta tttattgagc ataaaaatgt
    14581 cgagtattaa tacttttttc aatattaatc agagggcaaa agcatgcaat gttttcaccc
    14641 tcttgattat tatggaatag gataagtaga attttgattg attagtttac tatctcaata
    14701 aaattattgg caaatatttt gacaagatta ctttcaacag ctaaatttaa gttttgtcca
    14761 tcatcatatc cagcggaaat caatcctagt aagtttccaa acatatctaa aagagctcct
    14821 cctgaactac cactagagat tggagctgta aattgtatca tttgaaccgt ttcaaaatca
    14881 cgaaaagctg agataattcc atctgaaact gtattaaaaa gacctaaagg gcttccaatt
    14941 gcaactattt tttgacctct cacaggtttt ttactgactt taacaggtat tggtttgcat
    15001 tttctatcaa cctttatcac agctaaatca taatctgaat ggtattttat aatttggtaa
    15061 gatgtataaa ctttatcatc attttcaaat ctaactaaaa atgaataccc atcattgact
    15121 acatgaagat tcgtaagtat ataaccttca ttatttatta ctactccaga accactctta
    15181 aacggtttat tttcgttatt gcacacaaca atcataacaa cagaagctga taactttgca
    15241 aggtcttcat aagaaagttc tacttcctca tcttgtgcaa tttttttaga tgttagattt
    15301 gtgttattaa gtgtattttt agagttaggt ttattcgtag taggagaagt cttattggta
    15361 ttttcactgt aatttatatt tgtaatatgc ctttcaaaag gtatttctaa tatatttgga
    15421 gttttcctag aaagttcaaa tagagtagaa aattttgaac attcaggata taaatcaaga
    15481 atatctttat ttggatttat atataaaata tcacacccaa gttgtgataa aaaatataag
    15541 aaaaaaagtt cttgcctttt aatatttcca aagtatacta tctttggtga aatagtagtc
    15601 ttcaccttgt tataaaataa tttaggtaga aaatagtcca tccaacacaa aagtttaact
    15661 gcaaagtttt tgatgataga ggatgtacaa tttggcatac ttgtggaaaa taaatctaaa
    15721 gtatttttaa atgcaatctt tttagtccaa tttaaagtgt cattttctat tttacatgga
    15781 aagtcgatat tatataaatc ccaactgtta aacttagaac tatcataaat attaaaaatt
    15841 ttaagcatct tatctgtttg ttctttgtca aaatttattt ttagattgtt taatctaata
    15901 tagggaattg taggattatt aaaaaaataa gtatctatat ttttaacttt ttctatgtat
    15961 ttatcaattg aatcatcaac acctatatat cttgtaaagt ggacttcttt aatatatgaa
    16021 gaactcttat ttttataggt agtatcagat ataaattcaa ttagaggttc agttgacttt
    16081 tttattgaaa tctttaatgg tttaatagta caaatcatat tttttctcct ataataagat
    16141 gatataatct attataaaac aacttaaaac aatatagtac aataattttg tagtttcaaa
    16201 ataaaatatg tacttgttta tataaatgat atataattac atattaaaca tagcttttat
    16261 acatttaata aagtatgtaa aatctatgta aaaagtaaga aatataaaca tttttttaat
    16321 tatattttaa aaatatcaag caagttttct tcacaaatga ataaaaaatg ttataatatg
    16381 attaaagaaa aagataaata attagcaaga tttgtttagc taaaattaac aagggagcga
    16441 taaattatgg gtataacatt atcaaaaggc caaaaggttg atttaactaa aggaaatcca
    16501 ggtcttaaaa atattttagt aggtttagga tgggatacta acaagtatga tggtggattt
    16561 gactttgatt tagatacagc agcattttta acaggagcaa gtggaagtgt tactaatgat
    16621 ggagattttg tattttacaa caatttaaaa catacatctg gagcagttga gcatctagga
    16681 gataacagaa ctggcgaagg tgatggagat gatgaacaaa tagttgttga tttatctaag
    16741 attcctggag aaataagcaa gatttctttt actgttacaa ttcatgatgc gacagaaaga
    16801 agacaaaatt ttggacaagt aagtaattct tatataagaa tagtagataa agacacaaat
    16861 gaagagttaa taaagtatga attaggagaa gattttagta tagaaacagc tatagtagta
    16921 gctgaaatat ataagcacaa tggagaatgg aagtttaatg cactaggttc tggttttgaa
    16981 ggtggtcttg ctgcattatg tggaaacttt ggaataaact tataatttca actataacaa
    17041 taaactaaag ggggatattt tccatggcta tagtattaaa aaaaggacaa aaaatcgatt
    17101 taactaaggg aaatccaggt cttaaaaata taaaacttgg actaggatgg gatacaaatt
    17161 catttgatag tggctatgat tatgatttag atgtaagtgt tttcatggtt ggagaatctc
    17221 aaagagttga aaaagatgaa gattttatat tctacaataa cttaagacat ccatctggag
    17281 cagttgaaca cttaggtgat aatagaactg gtgaaggtga tggagatgat gaggaaatat
    17341 tagtgaattt agagacaatg ccaaagcata tacaaaagat agctgtaaca gttacaatat
    17401 atgaggctgc tgaaagaaga caaaattttg gacaagtaag caactcatat ataagagtat
    17461 taaattctga aaatgatgaa gaaatattaa gatatgactt aggtgaagaa tttagtatag
    17521 aaactgcaat tacagtatgt gagatatata agtataatgg agaatggaag tttagtgcag
    17581 ttggagcagg atttgaaggt ggcttagaag cactttgtag aaactatggc ttaaatgttt
    17641 aatttaagca atataatata ttaaatataa ataacctata caaataatgt ataggttata
    17701 ttatataaaa ttttgtggag gtttagtatg tcgattaatt tatcaaaagg tgataaaata
    17761 gatttaaaaa aatctaaccc aggactgagt aacatacttg taggtctagg atgggaccct
    17821 gtgcaacaat ctggaggagg tttttttaaa tcgttatttg gtggaggaca agctgatata
    17881 gattgtgatg catctgtatt tatgcttaat caagaaggta aattaagtgg aataaaagat
    17941 ttaatttatt ttggaaatct aaaaagtgcc tgtaaatcag ttttgcatac aggagacaat
    18001 ctaactggtg agggtacagg tgatgatgaa caaatcctag ttaatttgga taaagttcca
    18061 tcaaatattc ataaattatt gtttgtagta aatatataca attgtgtaga tagaaaacaa
    18121 cactttggaa tgatagaaaa tgcctatata agagttgaag accaaggtaa taagaaagag
    18181 atagcaaaat acaatctatc tgataattat tctgaaaaaa ctactcttat agtaggagct
    18241 atatatagaa aagatggaag ttggcaattt aaagcaattg gtgaaggaac aaaagatgcg
    18301 ggtcttaaag aagtaatgca aaatttagac aggattgagt gtgcatatgg aatttaataa
    18361 tgatatcaaa tattataatg tagataaaga agaagttaca aaagacttat ctgttaatcc
    18421 tgaaaaaggt ttatcagaat cagaagtcaa gactagaaga gaaaaatatg gtttgaatga
    18481 atttactcca aaagaagagg gtagtttttg ggatgattta aaagaaagtt taagtgaacc
    18541 aatgatagtt atcttgatta ttgcagcagt agtaagtgca gtaattgggg aaactcatga
    18601 tgcaataggg attgtaggtg ctatagcaat aggtatagca ataggtatga ttacagaagg
    18661 tcgttctaag aaagcagcag aggctctttc taagttgact gaaaacatag aagtaaaagt
    18721 attgagagat ggggaagtac atcaaatttc taagagtgaa ttggtaccag gtgatatagt
    18781 atatattgaa actggtgaca tggtacctgc tgatggtaga cttatagaaa gtataaacct
    18841 taaaattaga gaagatatgt taacaggaga atctgatgac gtatctaaaa agtgtgatgt
    18901 agtagtttct atggaagaga tagaatctaa aggagcagta gtagtacagg agccaatacc
    18961 tgcaaaacag ataaatatgg tgtttggtgg tacacttgta gcttatggta gaggagctct
    19021 agttgtaact tcaattggag ataatagtga aatgggtaaa attgctcaaa acttatcaga
    19081 aacagatgag caaactccac ttcagataaa acttggaaat ttaggtggtt tgatagccaa
    19141 agtttctagt gcagtagcag gattactatt tatatttatg gttttccaaa tgataagtaa
    19201 gaatgtatta aatgtagata tgtctggaat attaccgttt ttagaatcaa tagacccagt
    19261 taaaacagca tttacagtat gtgttgcgtt gatagttgca gcagtgccag aaggtcttcc
    19321 aactatgata aatatgacac ttgcaataac aatgcaaaaa atggctaaaa taaatgcatt
    19381 ggttaccaaa aaagaagctt gtgaaacgat aggttctgtt tctgtaatat gttcagataa
    19441 aactggtaca ttaactgaaa atagaatgac tgtagaagtt gcatatgttg atggcagata
    19501 tattgaaagt tcagaagagg aaataaatag ttattttgaa gaaaactgta tgataaactc
    19561 tacagctgat gtagagcaca atgatggtga tattaagtat ttaggaagtg ctactgaatg
    19621 tgcattgctt ttatattaca aaaacgtaga ttatagacaa gttagaaaaa attctaatat
    19681 agtagctcaa aatcctttta cttcagattc aaagagaatg acttctgtta taggtcaaga
    19741 taatcatcat gtactattat caaaaggagc accagaagtc ttacttgaat tatgttcaca
    19801 tattcaaaga ggaaaagaca tagttccatt aactgaaaat ataaaacatg agattttaga
    19861 agaaataaag aaattgcaaa taaaatcaat gagaacttta gggtttgcat ataaagaaat
    19921 ctcacaagct gaagaagaag cagcagtaac tgctgaaagt gatgctatga atgtaagtgc
    19981 aatggaaaat aatttagtat ttagtggttt tgttggtata agagacccac ttagaaaaga
    20041 tgttatagag tctgtaaata cagcaaataa agctggtgtt tctgtaaaaa tgcttacagg
    20101 agataatata aatacggcaa gagctatagg tgaagagtta ggcctattaa aaaataatat
    20161 gagagtggtt gaagcttcat atatagatac tttagatgat gaagaattaa aacaagagat
    20221 acaaagtatt tcaatcgtgg caagaagtaa accagatagt aaaatgagaa tagtttcagc
    20281 tctgcaaaag agcggggatg ttgttgcagt aacaggtgat ggtataaatg atgctccagc
    20341 actttcaaaa gcagatgttg gtatagcaat gggtatatct ggtacagagg tatctaaaaa
    20401 cgcagcagac ataattttaa ctgatgatag ttttagtacc attgtaaaag gtattaaatg
    20461 gggtagagga atttatgata acttccaaag attcgttcaa ttccaattaa ctgtaaatgt
    20521 aattgcattt ttagtggcta taatatctca agttatggga caagagatgc catttactac
    20581 aatacaatta ttatgggtaa acataattat ggatggacca ccagcactag cattaggttt
    20641 agagccagtt agagaccatg tgttaaatag aaaacctgtt gatagaaaag ccaatataat
    20701 ttctaagtca atggtgtata caatagtatt aaatgcattt tatataacag caatactaat
    20761 gttacaatct acatttaatt tcttaggagt tgatgaagct cataagggaa cagtaatgtt
    20821 tgcgttattt gcttttagcg cactgtttaa tgcatttaac tgtagagagt ttaatgcaga
    20881 tagtatcttc cctaatttta cacataacaa attagcacta caaataatag tgttgacagg
    20941 aattgcacaa gtaatattta ctcagctatt ccaagatttc tttaactctg tagcaatgga
    21001 ttttatgaca tgggtaaaag ttttatgtgt agcagcaagt gtaattgtgg ttaatgaagt
    21061 tgtaaaatgt ataataagac tggtaaaacc aaaaaagact gcttaaaaca aattttttag
    21121 aagaaaacag actgactcaa aatatgaatt ttgggtcagt tctgaataaa catttaaatt
    21181 gggaggatag ttatgggtga ctcaaaggta tttgaaaaaa tattctccag ccaatcagat
    21241 agaggaaatt acactccttc aaagggatat ctaagttatt ttatttcgta tattggttta
    21301 gaagatgaag ttttgtacaa tctcgagata tttaagacaa aacaaaatat tgatagcaaa
    21361 aaagatattg ctttatttac tgatgttata gcaaatccta gtgattttga tattataaat
    21421 tattttaaaa gtggacttca aaaatataga actagcatgg aagatgtaga tataaatatt
    21481 ttgggatttg aagagattga ttataaaata aagcaagcaa tggatagagt gttaaaagaa
    21541 gaggaaaaag aatttactaa tgatagagtt aaacaaaatt ttatagtgaa aataatggca
    21601 tggataaaaa tttatatcgg tgctttagat ataaataaaa atgaagctcc aaaagtaata
    21661 ttttatggag atataaaaaa acatgaagtt tatttactct taatattata cttggcaggt
    21721 tttgatgttt tatatcttaa tccaaattca aagagtaata tagatatttt aaaatcagaa
    21781 agatacaata tagagtttga agaagccaac ataatagaag aaaaaatttc ttttgaagaa
    21841 agggttattt taggggaaaa aatagataaa agttctgtaa aaaaagcttt tactgttggg
    21901 gcagaagcat caaagcgtat aagtgaggaa ttgttaaatg atgcaggatt tataaaacct
    21961 tggcaactcc aagatagaaa aataaaaaat ctacttctaa gttcaactgt tgatgaaata
    22021 tcaatatact ggaatcaacc acttaaatta agaccaggat ttaagtttaa tgatgctatt
    22081 gtagaagctc caaattttct ttctaagatt aatggaatat ataatgataa gaatgaatat
    22141 attaagtttt tagatttatt aagagattcg gaaagttcaa catttattga atttaatgga
    22201 gatgtagata gattttcaaa agcttttacg agagaagctt ttagtttatc ctttttattg
    22261 gattcaaaag gtgtaataga taaaaactct gttttaaata ataaagatta ttccatatca
    22321 acactagcac ttaatcaaca aattatgatt ttagagaaag tggaagagtt gttagaagga
    22381 agtatgtttt tgaatggttt aagtggagaa gacaagataa aaggtctatt tacagtactt
    22441 cacatggata agaaatttgt tcatatgatg aacaatttcg attactcact tataaaccca
    22501 aaattgatta tatacatgta caagagtatt gtatttgata aagaaatagt ttttcttatg
    22561 cttttactta gtaagattgg atttgatatt atcatattat gtccaggagg agaaaacaat
    22621 atagagaatg ttataaataa ccaattgatt gatatacaca gattggataa aatggtatat
    22681 gaccttaaat taaattcgct tgaaaatgat atacctttac ttaaaaaaat ttttggaaag
    22741 aggagacgat tttagatggg gataaatata aatagttttt caaaagataa gaactctgta
    22801 gaggttactg agaatttgcc tacagcagtt caagaagaaa acttcgatat aatggaatat
    22861 acaaataata aaaaaaatga actgagaaag tctaaggaag ttgaggcatt gacatccctt
    22921 atagaagtag aaaatccaga tacgatttta cagtttggta ggaaagcatc tgaaggagta
    22981 gcaagagttt ctgatagcct tttaaataca ataaaattaa atagaaatga agaaaattct
    23041 aaaatgctgg ttcatttaac taaaataatg gataagtttg atttagacga ttttcaagaa
    23101 actaaggaac caaatttcgt tcaaaaactt ttcaaaaaag ctaataatgc cattgaaatg
    23161 atgtttcaaa agtatgaaac attgggagga gaagttgaga aaattcaaat tgagcttgag
    23221 caatacgaaa gagatatagc actttctaat aagcaaatag gtgctatgtt aaatgaaaac
    23281 tttgagtttt ataatgaact tcaaaaatat attgttgctg gagaaatggc aatagaagaa
    23341 atggataatg aaatactacc tttctttaaa caaaaatcag agactagtgg agaccaaatg
    23401 gatgttgtca attatcaaga actattaaaa gtttatgata tgttaaatca aagagtctat
    23461 gatttaagaa tagcagaaaa tatagctatc caaacaatac ctatgcttag aggtatgcaa
    23521 cataataatt atggtttaat tagaaaaata aatagtgcat ttgtcgtaac tttaccagta
    23581 tttaaacaat gtttatctca agctatatta cttaaaaagc aagaattaca agcaaaatct
    23641 ctaaaagcac ttgatgacaa aactaatgaa ttattgctta gaaatgcaca aaatgtaagt
    23701 actcaaagtg cacaaatagc tagaatggct ggaacaagtt ctgttcaaat agaaacattg
    23761 gaaaaaatgt acaatactat caagtctggt atagatgaaa ctatgagaat agaagaaaac
    23821 aatagagctt taattaagga taatacaaag agattggaag aattaaatac gactataatt
    23881 tataataagt agtattatat taagggctaa tttgtataaa gttggccctt tctatgtata
    23941 tgaggaggct agacattgaa atatttcgat tatatctgca aagatgattt ggagagaatt
    24001 tttttgaagg agccagaaga ctttagtgct aagactgaaa aagatgtgtt gaaatatgca
    24061 ctgggcgctt ttttatacgt tccagctact caatataata tgatttataa gagtattata
    24121 ggggatgtta agggagttag accattagct atttgtttag aagatgctgt tggagtaaat
    24181 ggagagttag aggctataga aaatcttaga ttaattttaa aaaatattag caatgaaagt
    24241 ataactaata aagatggaat accactaatt tttgtaagaa taaaagatgt ggagcaactg
    24301 ttgagaatta aagagattat aattaaaaat agtcattcta taacaggaat attaattcct
    24361 aaagcaaatt cggagttgat tgaaaattgt atagaagctt tatattctat gaatcttcaa
    24421 gatatgtatg ttattccaat aatagagaca agagaattta tatataatga aaaaaaagaa
    24481 cttagtttta ctaatttgta caatgctatt ttaagacata agtcaagaat attaagcatt
    24541 agagtaggca taactgatat attagggatg tatggtataa ggagagataa aaacttctct
    24601 atatataata atttgatttg ttcttcattt atattagata taatcacata tcttcaaaga
    24661 ccagagttag atatacctat aagtggtgga gtatcagaat tttttgatat gacaaataag
    24721 gatataagaa ataagtatat agaagaaata ttattggata aatatcatgg attaattgga
    24781 aagaccgtaa ttcatccaat gcaaattcaa attgtacaag cgctatctac agtatcatat
    24841 gaagatttta ctgatgcttt ggatatactg gatagtacag acagtaagta tggtgtgagc
    24901 aagggtgtat taggagagag aatgaatgaa acaaatccac attttttatg ggctaaaaaa
    24961 acattgattc tatctaaaat atatggtgta ttaaataaag gagtagacta tgaagaattg
    25021 cttaaatttt aattgtgaga ttgacattat tgataatcca ttgaatttag aactaaatga
    25081 ttttttagac gtaggagtaa gaaataatag caaaagacgt tttttgttta tctcaaagaa
    25141 actcggtaaa catttagcat gtaagccaag tgagatggat aatttgggaa aattaatggc
    25201 tacaatttat aatgaaaaaa ataaggaaga atcaaatggt actgttatag cttttgctga
    25261 aactggtaca gctgttgggc atagtttttt tgattactta tgtggagatt atgaatttat
    25321 tcatactact agagagcagt ttgacgagtt agaaaatctg gaatttttag aggaacattc
    25381 ccatgcaaca gaccagaatt tatactttgg tatgcttaac aactttaaat ttggagatga
    25441 gataatattg gttgatgatg agattaccac agccaataca tgcatgaata taattaggaa
    25501 aattcaaagc atttatccta aaaagaaata cactatatgc tcaatactca attgggtaag
    25561 tgatgaaaat ttaagtaaag ttactcgttt agaaagagag ctaaattgta agatagattt
    25621 tgtatactta tttaaaggaa actttaaatt ttatatagat gaagatatta tttcaaatta
    25681 caacgataat tatagtatta ctaaattaga gactatttca agtgataaag taaatttagt
    25741 agtaaatcat atttatgttg atttagaaga ttatattgga aataagaaat atgtaaaata
    25801 tacaggaaga tttggaatta atagaagaga acaatttaga ttaaaagaaa tcgttaagag
    25861 agaaagtaaa aaaataacta tggccagtaa agaatcttta aaaaatgaaa aagaaaagat
    25921 tctattttta ggtactgaag aatttatgta tatacctatg ctgtttgcaa aagaaaaaga
    25981 aaatcaatat gatatatact atcattcaac aacaagaagt ccaattatta atattgataa
    26041 gaagcattat cctattaaaa gtaaatttat gctaaagagt ttttataata tagatattca
    26101 aaattatatt tacaatatag ataaacataa tttttctaag tgttatttat ttgtagagct
    26161 gaataaaagt gaaaattctt atctagaatt tattgatata attaaaaaaa caagtataaa
    26221 agaattaact atagtatgtt gtagctaaag tgttttaaca ggataaaata tatcacgatt
    26281 ttaagatagg agttgagtca tatgagagat tttagcacgt attctaaaga tgatgttatt
    26341 tttttattaa aagatatatc aaatttaatt gaagaagaag gaaataaaga aagagaatta
    26401 aaaatacaaa atggtagaca ttattcagaa atgataccaa tagagtatga agttagtgaa
    26461 gattatctga atttatatca tgagaaacta agagaaaata aagataagct tgcattttct
    26521 ataggagtta tgtgtgaaaa aataattaaa aaaaatggaa atgatgtagt actagtttca
    26581 cttgctagag caggaacacc tattggtatt ttggcaaaaa gatatataag aagcaagtat
    26641 aatgtaagtt tacctcacta tacaatctct ataataagag ataagggaat agatatgaat
    26701 gcaattgaat atatagtaaa gaatcatccg agctcaaaaa ttcaattttt agatggatgg
    26761 actggtaagg gaactatatc aaaagaactt gaaaaagcat gtgatgaact taatgttaaa
    26821 tttaaaaaac attttgactc aacacttgct gtattagctg accctgctgg atattcagga
    26881 ctttatggaa ttagagaaga tttcttaata ccaagtgcat gtttaaattc aaccgtttct
    26941 ggtttagtaa gtagaactgt actaagagag gatttaattg gtaaaaatga ctttcatgga
    27001 gctaagtttt atagacattt aaaagataaa gatgaatcta tgaactatat cgaaactata
    27061 gaagaatgtt ttaaaaatca atttaagaat atatctgatg aagttgaaaa ctgggaaagt
    27121 gacattataa caagagatgg atactttgat gttttaaata taaaagaaaa gtataacata
    27181 acagatatta actttatcaa accaggtgta ggggaaacga caagggtttt acttagaaga
    27241 gttccgtata agattcttgt taaagactta aatgataaat ctttagacca tatattcata
    27301 ttagctaagg agaaaaatgt tgaagttgaa caaatggatt taaaagccta taaatgttgt
    27361 ggaataataa aaaatatgaa agatatttag gaaatatatt agacaggaga atattgaatg
    27421 attgtttttt cagatttaga caggagtatc atttactcca ataaattttt aaatgctgat
    27481 agtaaatatg ctaatataga aatttatagg gaaaaagaaa tatcttatat atctttagat
    27541 actataaatt taataaagca aatacaatat tatggtatgt ttatacctac aactactaga
    27601 acagtggagc aatttaagcg tatagaattt aataaatatg ggatttattt tccttggagt
    27661 ataacttcaa atggaggagt tatacttaaa gataatgaaa tattgaaatc atggagtgaa
    27721 aaaatagata aactaaaacc tgattatgag cccattgaaa gtatgattca taaatttaaa
    27781 gactatttga atgtagatgg aataactaat tttaaagttg cagaagatac atttttttat
    27841 atagttgttg atttctcaag atttaatttg gattcaatca aagaatatac aaatatattg
    27901 gaatcaaaaa attggaagtt ttatgtgagt ggcagaaaaa tatattttat accaaaagaa
    27961 atttcaaaag aaaatgctat aaaatacttg actaaagaat taggaataga atatttttat
    28021 gcagttggag attctattat ggactatggt atgcttaata tctcaaataa atcctatgta
    28081 ttgaagcatg gtgatataaa taaaaatgaa atagaaaatt catttatatc aagttctttc
    28141 aaaggaatga gtggaactga agagatatta tctaacattt tagacgagaa ctgcctactt
    28201 aatatataat gactgatata taaataataa actagaaaaa tctgaaataa attatagtaa
    28261 agtttatcat actaatttat ttcagatttt tatcaattta tatttaagta agaactcaat
    28321 atgttaaaat gaaggtggag tcattgccca gactgagata catacctcat catcataatt
    28381 ttcatacaca tgtggtatag tactgttaaa ataaaaacta tctccttcct ccaatagata
    28441 ttccttattt ccaagtgtta tcttcatttt accttttatt atatagccac attcttctcc
    28501 ttcgtgtgtc acaaattcac tgtttttttg tgtatgacca tctagcgtaa caagtataaa
    28561 ttctattttt ttcccagata aattaggaac aagaagttca taaaattttg tagaatcatt
    28621 agttactatt ttttttcgat tatttttctt tactactata tcagagtcct ctcttccaat
    28681 ctcttcaaaa aaataaccta tatttatatc aagagctttt gatattttcc acattgctac
    28741 aatagatgga acaaccatat tacgctcaat ttgacttatt aagcctgtac ttaaatctga
    28801 attttttgct aaagtagaga tacttatatt tttagatttt ctaagagatc taattttgct
    28861 tccgacatct atattcatta gatacaaacc ccctcagaca tgattataac atataattta
    28921 taaagattaa taaaataaaa aaaagtttta taatgcatgt attttttatg ttatattgaa
    28981 ttttctgaaa agatgcatta taatagtatt aatattcaat atattgaata ttgataatga
    29041 tattttaatt taatgaaatg gaggtaatta agatgaagag ttttgtaagt cctgataata
    29101 tttgggtatt atgggcagtt gtaactggat gggcagcatt tagtatttac ttggaacaaa
    29161 agtacaattg ggcatcaaaa gtgtcgggag caataatagc tcttgtagga gcaatgttat
    29221 tatcaaatct aaatattata ccagtagagt ctgttgtata tgaccaagta tggggatatg
    29281 ttgttccttt agccatagct ttattgttat atcaatgtaa tattaaaaag atatggaaag
    29341 aaagtggaag attgctcatt atatttcttg taggttctgt aggaactgta cttggagcta
    29401 tgattggatt tttagcatta aaaaatgtag taccagattt aaatattgtt gcagctatga
    29461 tgacaggttc ttatataggt ggaaatgtaa actttgctgc tatgtcaggg gcgtttgatg
    29521 cgccaggaga attagtatca gcaacagttg tagcagataa tcttttaatg gcattatatt
    29581 tctttgtctt aatagctata ccatcaattg gattttttag aaaacatttt aaacatccac
    29641 atgtagatga aatggaaagt ataggtataa ctgaaggaga aacagtagca gcaaattatt
    29701 ggggaagaaa agaaatatct ttaaaagata tagcaatagc cattggtagc gcctttataa
    29761 ttgtcgcagt atctgtagaa ctatcaactt ggtttaaagc tataatacca ttatcaaatc
    29821 cttttttagc aatgttaaat actttatttg gaaatcaata tctaataata actactttga
    29881 ctatgttgtt tgccacattt atgcctaatt tctttggtga tataaaagga gcacaggaat
    29941 tgggtacatt tttaatatat atattctttg ttgtagtagg tgttcctgct tcaataacat
    30001 taataattca aaaatcacca ttactacttt tatactgcgg aataatggta ctaataaaca
    30061 tgctagttac atttatagtt gcaaaaatat ttaagtttag tttagaagaa gctattcttg
    30121 catctaatgc aaatattggt ggtcctacaa cagcagcagc tatggcaata tcaaaaggat
    30181 ggtctaagct agtagggcca atacttatag ttggaacatt tggatacata gttggtaatt
    30241 attttggtct tttagtagga aatattttga tataatttta cgtgaaaaga agttgttttt
    30301 tagaaagatt tttatttaat tggaggttgt tatgattttc gatggacatt cagatacttg
    30361 ggcagatgta acaataagaa ggcttaacgg ggaaaaagat gtatttagaa gaatccatct
    30421 tgataatttt aaaagagcaa atatagaagg agctattttt gtaatatgga tagacccacc
    30481 atatgataaa gagcctgaaa aaagagttaa acaaattata agctctataa aagatgaaat
    30541 tgaagatatg aaagatgttg taaacatagt aaaaaaatat tcagatatag ataaaggtat
    30601 aagtgaaaac aaaataaata ttttaattgg tcttgaagga ttaagccata ttggaagaaa
    30661 tatagattta ctagattatt tttataaaga agttggtgca agacatgcat cattaacttg
    30721 gaatgaacag aatgaacttg ctacaggatg tttaggggat gaaaatcgtg gtttaactga
    30781 aataggtaaa aaagcagtca ataaaatgga aaaattgggc atgatagtag atgtgtcaca
    30841 tttaaatgaa aaatcatttt gggatttaat gtctgttgca agtaagccag ttatagcttc
    30901 tcattctaat gctaggagtt tatgtgatgc taaaagaaat cttacagata atcaattaaa
    30961 agaaatttca aatacaggtg gattagttgg aataaatagt tttagaggat ttactaatag
    31021 taatcctaag aagcaagatt taatacattt ggttgaccat atagactata tggtaaatct
    31081 tataggtata gaacatgtat cttttggttt tgattttagt gattacttag ggaaagagac
    31141 tataggttct tttactggaa atgctagtgt gccaagtgta gatggtcttg aaagcacaca
    31201 tacagcacag aacatagtag tagagcttga aaatagaggt tataaaaaga aagatataga
    31261 gttaatctct agtggtaatt tttataggct ctttaaagat atactaaaat aatataataa
    31321 agaacttaaa tttataaaat agtatattta tacaataata tctacatatt taaattggcg
    31381 ttcataattt attaaattaa attttaatgt tactagaagt ttcaattcta gtaacatttt
    31441 ttatgttaaa aatagatata gatatatatt gacaatgata attattattg ataaactata
    31501 aaagtgaaaa tgataataat tttcatttta attaataaaa gatttttaag gagggagaat
    31561 gaagaaaagg tatttagtca taggaataat aattttgtct tatttttctt tatttatagg
    31621 agctgaagat ataaatataa tgcatatatt tgcaaaaaat cagcataagc ttatgatatt
    31681 tatcatgagt agggtaccaa gattaataag tatattgatt gctggtgtag gaatgagcat
    31741 agcaggtctt ataatgcaac agataagtaa gaataaattt gtatcaccta caacaggtgc
    31801 tacaatagat gcagctcaat ttgggatagt aatatgtatg cttttagtac caactgcctc
    31861 tatttttaca aagacaataa tagcatttgt attttctctt gtagggactt ttacgtttat
    31921 gaaaataata gggaagttac aatttaaaaa tataatcttt gtaccattag taggtataat
    31981 gtttggaaat ataataggtt ctatgacgga ttttatcgcg tataaatacg atttgagtca
    32041 aaatgttagt tcttggatgc aaggtgactt ttctatgata cttaaaggaa attatgagat
    32101 attatatatc acaatacccc ttataatatt agcatatatt tatgcaaata aatttactgt
    32161 tgttggtatg ggtatggatt ttgcgacaaa cttaggtctt agttacaaaa gaattgtcaa
    32221 tataggtctg ataatagtag cacttgttac agtatgtgta gtcgttacag ctggtaatat
    32281 cccttttata ggtctaatag ttcccaatat cgtatccttg tatatgggtg ataatataag
    32341 agcaagcata tggtatactg gtttacttgg agcggtattt gtacttatat gtgatatttt
    32401 tggaagaatt attatttatc catatgaaat atctatagga cttacagttg gagttatagg
    32461 aagtatacta ttcctttatt taatattaag gaggaatgtg aatgaagctt agtctcaatc
    32521 aaaaaattta ctttgtaagt atattactat tcatatcaat agcacttttt ataatttatg
    32581 gaatagacat gaatcattta gaatatgcat tatcacaaag aataccaaaa atatttgcta
    32641 tggtattagg tggaggatgt atagcattta ctactgtagt gtttcaaaca attacaaaca
    32701 atcaaatatt aacaccaagt gtactaggtt tagattcatt gtatgtaatg attcaaacta
    32761 taatagtatt catatttggt tcaagcagta gtctgattat aaatgaaaat tataatttta
    32821 ttattaatgt agctatgatg attggagctt cattgttatt atataagatg ttatttgaaa
    32881 aaaataaaaa taatattttc tttttaatcc tagtaggtat gatatttgga accttattta
    32941 aaagtgcaac aacatttatt caagtaatga tagacccgaa tgaattttta gctttacaaa
    33001 ctagtataat ggcaagttta aacaatataa acactaatgt attgttaata gcatttataa
    33061 taattattgc cataatacct tttatatatg atgaaataaa gtatttagat gtattgtcgt
    33121 taggtaaaga acaagcaatt aatttaggtg tagattttga taaggcagtt aaaaaaatga
    33181 taatattaat agcaatttta gtctcaatat caacagcact tattggacct atgacatttt
    33241 taggactttt acttgctaat ataacaagag aaatttttaa aacatataag catacatact
    33301 tgatttcggg ttctatgttg atagggatga ttacattgat aataggtcaa ttttttatac
    33361 aacatgtatt taagtttgat acgactttaa gtgtagtaat aaattttata ggaggaattt
    33421 actttattca attactatta aagggggcaa atagatgata gagataaaaa atatatttaa
    33481 aagatataag aataaaaatg ttgtagatga tgtctctttt agtatagaaa agggaaagat
    33541 aacatctttt ataggaccaa atggagctgg aaaaagtaca gtactatcaa tagttacaag
    33601 gcttatagga ggtgatggcg gagaagtaat aatagaagga aaaagcttga ctaattatag
    33661 caataaagaa ttagcaaaaa aaatagctat tcttaaacaa tctaacaata taactttaaa
    33721 gcttacaata agagaattag ttggatttgg aagatttcca tatagtgaag gtaatctaac
    33781 taaagaggat gaaaattata ttgatgaagc aatagaatat atgaagttga ctgatataca
    33841 acataagtat ttagatgaat tgagtggagg tcaaagacaa agagcttata tagcaatggt
    33901 aatagctcaa gatacagagt acatattact tgatgaacca ctgaacaatt tggatatgaa
    33961 ccattccgta cagatgatga aggtacttag aagtctttgt gatgagttag ataagactat
    34021 agtattagta atgcatgata taaattttgc atcatgttat tcagataata tagttgcatt
    34081 aaaaaatgga aaagttgaaa aagtaggtcg tacagatgag atagtaaatg aaaaagtttt
    34141 agaagatatt tatgagatga attttaacat taagaatata aatggaaatc gaatctgtat
    34201 ttatttttaa aatattgtat atgaaaataa gcttgctata tgatttttta taaagttttt
    34261 aaattaaaat aaagattaat aggagagagg aaaatattga tatgaataaa aaagcagcaa
    34321 tagtagcagc agtagctatt ataggattag tgacagtgtt tgcattaggt ggaagtaaaa
    34381 agaatgaatc aaaaacatca gaaaattcaa ataataccat aaagataact cataacttgg
    34441 gagaaacaga tgtaaaatta aatcctaaaa aagtagtagt atttgattat tcggctttag
    34501 atacaatgga tgcattaggt gtagctgaga atttagtagg acttccaaaa acgagtttac
    34561 cagcatcttt agaaaaatat aaggataaga agtatgcaga tttaggaggt ttaaaagaac
    34621 cagatttaga aggtataaag tcggcaaatc cagatttaat tataataaat ggaagacaag
    34681 aagattttta tgaacaattg tcaaaaatag caccaacaat aagtacaagt aaagatgata
    34741 agaagtactt agaatctgtt aaaaataata tagacaaaat agcaaaaata tttggagtag
    34801 aagaaaaagc aaatcaagaa tttagtaaaa ttgaaaagaa aatagaaact ttgaataaaa
    34861 aagtaacaga taaaaattta aatgctttaa ctataatggt aaatgaaggt aatttaagtg
    34921 tatttggtga agaatcaaga tttagtatat tatacaatag ttttggattt gaaaataaag
    34981 ataaaaatat aaaagaatca agtcatggac aaaatattac ttttgaatat atagctaaac
    35041 aaaatccaga agttatgttc gttatagata gaggaatagc tactggtagt gatgtaaaag
    35101 aaagctcaac tgcaaaatct gttttgaaca atgatataat aaaatctatg gatgcatata
    35161 agaatgataa tataatatat ttagattcac caacatggta tgtaaatgat ggtggtctaa
    35221 catctttaaa taaaatgata gatgatgctt caaaagcggt aaattaataa tccgatataa
    35281 tcaaaatgta ttttttgtaa aatgattatc aaataaacta ggagttttta agaactccta
    35341 gtttattttt tgcataataa agatatagtt taaacaataa tattatttat aataagataa
    35401 atctattcaa aatctaataa aatatgaagt tatactagac aaaaagaata actcttaaat
    35461 ataattaagt tatataataa
//
LOCUS       DAMKPG010000273        20764 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.074, whole genome shotgun sequence.
ACCESSION   DAMKPG010000273 DAMKPG010000000
VERSION     DAMKPG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20764
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.074"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            196..1794
                     /locus_tag="PC621_003919"
     CDS             196..1794
                     /locus_tag="PC621_003919"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF4110977.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(2029..2220)
                     /locus_tag="PC621_003920"
     CDS             complement(2029..2220)
                     /locus_tag="PC621_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF4110978.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(2389..2982)
                     /locus_tag="PC621_003921"
     CDS             complement(2389..2982)
                     /locus_tag="PC621_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HDF4110979.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(2983..3129)
                     /locus_tag="PC621_003922"
     CDS             complement(2983..3129)
                     /locus_tag="PC621_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HDF4110980.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
     gene            complement(3807..4331)
                     /locus_tag="PC621_003923"
     CDS             complement(3807..4331)
                     /locus_tag="PC621_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF4110981.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(4396..5148)
                     /locus_tag="PC621_003924"
     CDS             complement(4396..5148)
                     /locus_tag="PC621_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF4110982.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(5179..6477)
                     /locus_tag="PC621_003925"
     CDS             complement(5179..6477)
                     /locus_tag="PC621_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF4110983.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(6494..8758)
                     /locus_tag="PC621_003926"
     CDS             complement(6494..8758)
                     /locus_tag="PC621_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF4110984.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(9013..9831)
                     /locus_tag="PC621_003927"
     CDS             complement(9013..9831)
                     /locus_tag="PC621_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF4110985.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(9824..10522)
                     /gene="cbiQ"
                     /locus_tag="PC621_003928"
     CDS             complement(9824..10522)
                     /gene="cbiQ"
                     /locus_tag="PC621_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF4110986.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(10525..10809)
                     /locus_tag="PC621_003929"
     CDS             complement(10525..10809)
                     /locus_tag="PC621_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF4110987.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(10799..11551)
                     /locus_tag="PC621_003930"
     CDS             complement(10799..11551)
                     /locus_tag="PC621_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF4110988.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(11740..11932)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12064..13836)
                     /locus_tag="PC621_003931"
     CDS             complement(12064..13836)
                     /locus_tag="PC621_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF4110989.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(13850..14779)
                     /locus_tag="PC621_003932"
     CDS             complement(13850..14779)
                     /locus_tag="PC621_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF4110990.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            15008..15196
                     /locus_tag="PC621_003933"
     CDS             15008..15196
                     /locus_tag="PC621_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF4110991.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            15189..15890
                     /locus_tag="PC621_003934"
     CDS             15189..15890
                     /locus_tag="PC621_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110992.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            15892..16815
                     /locus_tag="PC621_003935"
     CDS             15892..16815
                     /locus_tag="PC621_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4110993.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            16972..17895
                     /locus_tag="PC621_003936"
     CDS             16972..17895
                     /locus_tag="PC621_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4110994.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            17888..18625
                     /locus_tag="PC621_003937"
     CDS             17888..18625
                     /locus_tag="PC621_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110995.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            18636..19409
                     /locus_tag="PC621_003938"
     CDS             18636..19409
                     /locus_tag="PC621_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4110996.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            19425..19577
                     /locus_tag="PC621_003939"
     CDS             19425..19577
                     /locus_tag="PC621_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4110997.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(19950..20558)
                     /locus_tag="PC621_003940"
     CDS             complement(19950..20558)
                     /locus_tag="PC621_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF4110998.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         6841 a         3472 c         2633 g         7818 t
ORIGIN      
        1 gattctatgt ttggttgggt aaaaatatat ataataataa tttttatatt aaatttgtag
       61 tatttatgta agaaataaat tttatataat attttttcac taaatttttt tctttttaca
      121 acaaatactc actacctttt gatataatat ttttatcaat tattattata ttcaagtttt
      181 gaaaggagta ttaaaatgag tttattagtt ttcggacata aaaatccaga tacagattca
      241 atatgttctg caatttcatt agcacattta aagaacaaac taggaataaa agcaacagct
      301 tatgctttag gagatatcag aaaagaagct aaatatgcct tggattactt taaggtagat
      361 gcacctgaaa ttttagataa tgttagaata caagttagag atttaaatta tgataaagta
      421 gtacctttaa caccaacttc atctatatta gaggcttata accttatgga tgaaaaaaat
      481 gttaaaacac tacctgtaac ttatgatgat ggaacttttg ctggtatcat tactatgaaa
      541 gaaatagcta aaaatctact tcatcaacac tttacaacta tacacacatc actatcaaac
      601 atttgtaaaa accttaatgg tactattctt gtggactgtg gtgaagatat aaaaggtaga
      661 gtagtcactt tatcatttgg aatggagact ctaatagaga ctttaaaaga aggagacata
      721 gctattgttg gagatagata tgatagtatt gaatatgcta ttgatacaaa agttaaactt
      781 ttaatactta ctaatcatac agagatttca aaagatttgc tagctcttgc taagataaat
      841 ggagtgtctg ttgtttctgt tgaaagtgac aattataaaa cttctaatgt aataaatcaa
      901 tgtagtttct tagactctat agaagctaca gaaaatttaa ttacattcaa agaagatgat
      961 tatatggatg aaataaaaga gattatgcta gaaacaaact tcagatctta tccaatatta
     1021 gatgatgata ataagttttt aggtttagtt tcaaaaggtc atctattaaa cccatctaaa
     1081 aagaatgttg ttttagttga ccataacgaa tatgctcaaa gtgctgatgg aattgaacaa
     1141 gcaaacatag ttgaaataat tgaccatcat aaacttggtg gtatttctac tgatgttcct
     1201 atgtcattta gagttatgcc tgttggatgc aatagtacta taatttatca aatgtacaaa
     1261 gaaaacaatg ttgagatacc ttatgaaata gctgggcttc ttttatctgc tatattatca
     1321 gatacattat tatttaagtc tccaacaaca actgatatgg ataagaaagc ttgtgaagaa
     1381 ttaagtaaaa tagcaaaagt agacatggaa aaatacgcta tggatatgtt taaatgcggt
     1441 acttctttag atgaatatac tatagaagaa atagtaaata tggattttaa agaatttaat
     1501 atgagtggac atagagttgg tattggacaa gtatttacct tagacataga ctctatattt
     1561 gctaaaaaag atgagttttt atcttatata aattcgactg attatgacaa actaattctt
     1621 gcggttacag atataatcaa agaaggttct tatttaatct ataaagcaga agatagctta
     1681 attgctaacg catttgatgt tgaaggtgtt caaggtacat ttgcaccagg tgttgtatca
     1741 agaaaaaaac aattagtccc taatttaact actgcaatca aaaattttaa ataatataaa
     1801 atttttaaat aacgaaaact gtctcaaatc tatatttgag acagtttttt attatacata
     1861 tattgataat aaagttaaat aaaaatccat aaatttaaaa tttttatgaa tatattttat
     1921 tattattgtt attttattca ttctaaaata ggtaaaactc aatatataaa aatacttttg
     1981 tcatttaaat aaaacctaaa tagtttttaa gattatttta gacacgtttt aagtcataac
     2041 agtatcagct attctaaata tcgtcaaagt agccgcagtt cctgctgatt gcccatttcg
     2101 tatccctact gttgtagatg aaaaattatt tattgtaaaa gtagtccccc caaataaaaa
     2161 accagcaaaa tatccagaaa gtactcctcc gcctcctggc tcagtagtaa gccctgccaa
     2221 atcttttcct cctaaaattg ggaataggat agcaaaagaa tattgattta tacttgatga
     2281 acctggttgt aatgctactg aaactgatat ataatacaca ccattttctt gtactgtaaa
     2341 cgtaccattt gcatctactg ttatacttga actatttcct attattgttt accaacctgt
     2401 tatagcattt ccattattta gtgtttgtgg agatggtgca tttacgagga agttagcaat
     2461 tggctgagag gaattgcctg ttgctcctgt tggacctgtt gctcccattg gtcctatact
     2521 tcctgtcgct cctcttggtc ccattggacc tgtaattcta attggaagac actcacattt
     2581 aggataacaa ttatctataa ttctatcttc aaatgttaca agtatatacg gtttgttaca
     2641 atccctacta gatgtaaatt caaaaatctg ataagggtaa tttaaagata aagctaaccc
     2701 ataattagga tatttattca ttgcccatgc ttttatagtt cccgtaatat ttaaacaaat
     2761 atagttggat gtatcactta tcccaattgg taaatatccc ctgatatttt ccatagacac
     2821 tctatcattc catgttatag ttcgtaaatt ataatattca cttattcttt taatttcaat
     2881 atctactttt tctttaaaaa tatgattctt atttgaatca atatgaataa ataactctgc
     2941 ttttaatata cttttgtctt ttaatatatc tggtattctc acttaaaaaa aagtaatcaa
     3001 cttttctttt acaaaactat ttgaacattt acttcctatt aagcaataat ctaaattact
     3061 aaagttttta tctggatatt ttttagatat aaaagtatca tcatttaaat aaagtataat
     3121 ttttctcatt tataatccct ccattttggg tctatatcat actatgttta aaatacatgc
     3181 ttagttacaa tacaattaaa atttatagta ttaaattttt atataataat gctgatttct
     3241 acaaaaaaag aacatgtcat tagaaacttc taatagcatg ttctttttca ttattatata
     3301 ttacacttta aattattcct tgactggctt agctggtgta aactaaacca gtctttctaa
     3361 aatattaagc acttttttct tattatgttt tttaggattt attgtctcta ttattgtaaa
     3421 ccaaagttat aaaattgtaa atagaatata ttaccataac aatatttttt tataagtaaa
     3481 aattataaaa aaccttattt ttattaaata gaataaataa aataagttta aaaaatactg
     3541 aaataagtgt tttccacata ttttatatat ttatttaata cattattatt taaaaataaa
     3601 tatataaaca atcataataa aattttttta ataaaacttt attttttagt taaaatcata
     3661 caatatatag ctttccacaa tacaaataaa ttaaaaagtg aaatgtattt gtctaaaaac
     3721 ataataacat ttcactttat cctcattttt aagtaatata tatttttaat ttttatttat
     3781 aactcaatct agtttttata taagttttag tttttttcta catctgagta atctattcca
     3841 aaagtttcta cagtaacaga cttcataatt tgtggagttt gtggcttatc agctgaatct
     3901 gttctaactg atgctatttc atcaactgta tccatacctt ctatcactct accaaaacct
     3961 gcatactgac catctaagtg tggtgagttt ttatgcatta tgaaaaattg agaaccagct
     4021 gaattaggtg ccatagttct agccattgat atgactcctc tttcatgttt taaattgtta
     4081 gtaaatccat taccactaaa ttcacctttt attgagtgac ctgggccacc cattcctgta
     4141 ccatttgggc atcctccttg tatcataaat cctggtatta ctctatgaaa tattattcca
     4201 ttgtaatatc cttcatttac taatgataca aagtttttaa ctgtatttgg agctatattt
     4261 ggatataact caattttgat ttcttttcca ttttccattt ctatagttac tataggattt
     4321 ttattttcca taatttatta ccttcctttt cattagttaa attacataat tacatttatt
     4381 atattatact ctaaattaaa gtctatcaag tatatttttt cctatttcgt atccttcttg
     4441 ataaatagtt ttacaaaact catcatcaaa gttaagcgta tctttaggag caaattgagt
     4501 attaggtcta actactatta ttttatcttc atcatatact tttttaatat cctctggcaa
     4561 ctcataatta tattttgtgg atattaaaat aattttatcc acattatccc caattaatgg
     4621 ttcaattaat gcactcctaa ttaaacctcc atctaatttg aaaccatcat aaattccttc
     4681 ttgcacatct ttcacaaatt gttctttaaa tccaagagtt gcgtttggat ttgatggtaa
     4741 caacccactg tatgaaatgc tgataatggc ttcattacga ggcatttttg atatgtcaac
     4801 aatcttttct gatactactt tattatcaac ttcaacataa ttaacataaa agttcatttc
     4861 ttctatatta ctatcatcta gttcccttag gttgtctata gcaggagaat tatccacagt
     4921 attatttact atttttacat ttccattctg gatatctttt atatttgtcc acattttctc
     4981 caagttatca acattccctg tgtacaaata atatccattt attgctccta ttgatgtccc
     5041 tgaaatcgaa taaaatttat ttattcctcc atcatacaag gctttaacta ctcctgcttg
     5101 ataagcccct tttgctcctc caccttctaa acataatcct aatttcatag tctcatctcc
     5161 aaattgttta tcttatattt atatttcaaa aaacttacta aatgttttat atatactata
     5221 gtggtcctca acatttccta attctcttat agagtgcatt gctaaaattg gacttcccac
     5281 atccacagat ggtatatcta tatgagtaga agatattgga cctatagtac ttcctcctct
     5341 ttcatctgac ctattaacaa attcttgata ctcaacatta acttctctac atatattttt
     5401 ataaatagct atagaatatc catcactagt atatgcctga ctagcattta tctttataac
     5461 tggacctttt cccattattg gtttattagt tgggtcatgc ttttctggaa tgtttggatg
     5521 aatagcatga gctaaatctg atgatatcat aaaagatgaa taaatagact caaagaatcc
     5581 ctctctatcc ttacccattg atatgcaaat tctctccaat atatttaaca acatgtttga
     5641 atctgcacct tgtttagtag agctaccaac ttcttcattg tcaaatacag ccattacatt
     5701 tacaccttgt tgacctttag tattaacaag agcattcaaa ctagcatgta ccattgcaag
     5761 attatcttgt ctcccaatag atataaattc atcattagcc cctattaaac ttcctttttc
     5821 aaattcatat aaaaataaat caaaatctat aatatcttcc atatttacat ctaagttaga
     5881 tgctatttgc tttataagaa aattatcttt ttctaatgta tcatttaaca tcccaactaa
     5941 aggtaacata tctttttgct tattatatga atgaccatca tttatacttc tatttaaatg
     6001 tattgctaga tttggtatta tacaaattgg ctttttaaag tctataagcc tttcatttgg
     6061 ttttaatata ctatcacctt ttaaaactac tctaccagct atcccaagag gtctatccaa
     6121 ccatgtactt aatattgctc ctccatagca ttctgtattt aacttcaaat aagatttttc
     6181 agcttctatt tctgcatttg gtttaatact aaatgtagga gaatctgaat gagagcctat
     6241 tattctaaat ccatctcttt ctatatcttc tgaatttact acaaaagcta ctatagctga
     6301 tagattctta gtaacataat actttccacc aactcttaga ttccatcttt gattcaaaac
     6361 taattcttca aatccatttt tatctaagat ttccttagta ctctcgactg catgaaaaga
     6421 cgacggacta tcatatatga agtctattaa gtttttagca aagtttatac tatccattcc
     6481 tcatttacct ccattataaa atatttagtg ttgtatattc tttatttatt tttttaattc
     6541 cctgttggtc aaaagctata gtaacatctg taccaattat agaaaccact gtcccaaccc
     6601 caaatttagg atgatgtacc ttagaaccaa gttttatatc atctggatta gtttctttat
     6661 tagaatcttt tattgtagca ttaactttat ctgcaacttt agatttgctt atagtactca
     6721 tatacttttt agtatattta tctaacatat tataacttgc tttagaatag cttagttctt
     6781 tttttacttt gtacaatctt tctatacatt cttctggcag ttcttccata aatcttgaag
     6841 ctattgcaac atttgtttta ccatataatg ttctcttctc tgtcaatgac atatatagct
     6901 cttctttagc tctagttata ccaacataac aaagtcttct ctcttcttct atttgtgaat
     6961 cactcataga ttttatagct cttgatatag gaaataaccc ttcttccata cctgttaaaa
     7021 acacaacagg gaactctaaa cctttggatg tatgaattgt cataagcgaa accctatcat
     7081 tgtcttcttc atcatttgat tcagatgtaa gtgctatact tgttaggaat gtctctaagt
     7141 ctttattttc ataagtatca tcattactac tttcaaactc caaagctata gaaataaatt
     7201 ctctcaaatt atcaattctg tcttgagctt cttcaccttt gcctgttaaa tctttttcat
     7261 ttttatttct tatctctaca agctcgtcca tatatcctgt agtatccaag actttttcta
     7321 taagtttgct aacaggataa acttctttta tagttctaag agtccctata atatcaacaa
     7381 atccacttat acttgccctt gccttagtag atatatctga atttgtttct atatcaatta
     7441 atactgagta tatactttcc tgttttaagt tagctctatc ttcaattttt tctatagttc
     7501 taagccctat acctcttcta ggaacattta taatcctttt tatagatata tcatcttgtg
     7561 gattttgaat aactcttaaa tacgccaaca aatcctttat ttcttttctc tcgtagaatt
     7621 ttgtaccacc atatatattg tatggaattt gacttctatt taaagcgtcc tctacaggtc
     7681 ttgcctgtgc atttgctcta taaagtactg caaaatcctt ataaggtcta ttttcttttc
     7741 tagctatttg tgcaattgaa tcagcaatga aatcagcctc ttctatttcg ctaccagtta
     7801 attgtatctt tataagttct ccttcttttt tttcactcca aagctttttt ctttttcttt
     7861 ctatattatt agatataact ttattagctg catctaatat cacttgtgta gacctataat
     7921 tttgttctaa ctttacaaca tgtacattgt cataatcttt ctcaaattca agtatgtttc
     7981 ttatatctgc gcctctccat ccataaatac tttggtcatc atctccaaca acacaaatat
     8041 tttgatgttc tcttgctaat aattttatca attcatattg agctttactt gtatcttggt
     8101 actcatctac cattatgtat ctaaatctac ttctgtaata tgctaatact tcatcattag
     8161 ccttgaaaag ttctacagtt ttaagtatta aatcatcaaa gtcaagtgca ctatttcttt
     8221 ttaaacggtc ttgatataac gcataaatat ctgcaatctt aaccattcta ttatctgcca
     8281 tgttgatatc tttaaactgc tttggagtat aaagtttgtc ttttgctcct gaaattgcac
     8341 ttataactgc ttttggttca aacactttat cacttaaatt taattctttt agacagtctt
     8401 taaccaatgt aacttggtct gctgaatcat aaattacaaa acttctattg taaccaatct
     8461 tatttatatc ttttcttagt atacgaacac agcaagaatg gaatgtactt atccacatat
     8521 ccttagtttc aggacctata ttttgttcaa ctctctctct catttcattt gcagctttgt
     8581 ttgtaaatgt tatagctaat atatttggtg cttgtacacc tttatcttct ataagatatg
     8641 ctattcttgt tgttaatacc ttagttttac ctgaaccagc accagccagt ataagtactg
     8701 gaccttcagt cttttctact gcttctcttt gagcaggatt taatgtatct aaattcatat
     8761 ttctcctcct gttaacagcc ataaatatat tttaatctaa aatatgaatt acttcaaatt
     8821 ataattatgt attatattaa tagattattt ccaatatatt acaattaaat aacatttttc
     8881 catataattt atttaattgt ttaataaaaa aaataacctt atgatttggg gaatcataag
     8941 gcacctttaa ggggtctagg ttaagggtta ttatatataa atctattttt ttaaaatagg
     9001 tttagggctt aattatttat ttgtattaag ttctctttcc ttccaatact tatacaaatc
     9061 ttcttctttc ttaaataagg gtaaattcat atttctatgt acttttacta accatgggga
     9121 ttctaggcca gcttgtataa tgttattttc atttacaaat acattttctg cctttccttc
     9181 atcaattaaa atccccttat ccaatacata tatataatcg catatttcat acattaaatt
     9241 catatcatga ctggatatta ctatttttat attatcttta ttcaatccct ttattatatc
     9301 tacaatagac ctagtactta ctgggtctaa accagctgta ggctcatcaa gcaatataat
     9361 ttcattttcc attgctataa ctgatgctat cgctaccctt tttttctgac cataacttaa
     9421 aaaatgtact ggtctatcta taaaatctat tccattaaca gctactagag ctttgtttat
     9481 tctctcttta attatttttt catctatacc aatatttctc attgcaaagg ctatatcatc
     9541 atatactttt gaataaaaaa tttgtttttc tgggtcttgg aacacaatac ctacattttt
     9601 tctaaggttg tataaacttc tcttatcata ttttaacttt tcttctttaa aaagaatttc
     9661 tccagatgta ggctttaata tacccattaa attcataaat aatgttgatt ttcctgaacc
     9721 atttgaacct attataccta taacatttcc ctttgaaaaa tccatattaa tatttaatag
     9781 tgcattggta ttcttttcat attgataagt taaattattt attttaaaca tcattatccc
     9841 ctactatgtg aaatttacca tcatacaatt ttatgtctaa tgatatacac atatcatcat
     9901 accttgtcat aagtcttttg tacagcatac tgccaagaat tcctaatgat ttatatgaag
     9961 tttttaaatt tatgtaccca aatcttagtt cttgtgactt atatatttct gaaaattctt
    10021 ctaaaaatat gaatataaac ctatatatta acattgatag ctcaacaaaa gtatcaggca
    10081 aatgcatttt tttaaagaag aatattaatt gattaaaagg tgttgtaagt atacaaaagt
    10141 atacacaagt caaacaagac atagctctaa ataacacatg aatagacatg tcaatagatg
    10201 cttttgatat tcctacattg aaactaaaaa tttcaaaact atataataag tctgccttat
    10261 taaaagatat atttacaaga gttattccta tactaagaaa taaaaagtac attggtattc
    10321 tgagtaattt taaataattc tttaaatcta ttccagctac acaaacaact agaatactca
    10381 ttaaaatcat aattccactt aaaacaaata tattttttat taccatagaa gcaatcagaa
    10441 agattactcc tatggctact tttttatttg gatttacttt acttaatcta tttgtatatg
    10501 catatttatc aattataagc ataattaact tctctttcct ttttgaacac ctaatatgta
    10561 ccctattaca ccagcaccta acgctgcttg tgcagagaat aataaacttt ctatttctcc
    10621 acttggtggc tcatatgggc tactaaacca tggttcataa tctggattta tcttagtaat
    10681 ttcactctca gcttgaccat ctgctcctcc atattcagct cccgaattaa caagtaatgg
    10741 gaatatgata agagctacta ctaataaaag tagtaatata ttagttttcg tctttgtttt
    10801 agcactcatt agctttaact cctttatcat tattttctag tatgcttttt tctccatttt
    10861 ctgtaattaa attatataca attacagtaa gaataccctc tgctattgct attggtattt
    10921 gagtcataaa gaaaattcct aaaaacttaa ttgctgaagc catcactcca cctgatgggt
    10981 ctgggaaagc cagtgctaac tgtatcgaag ttactgtata tgtagccaaa tctcctattg
    11041 ctgctgcaag aaatactggc attgcattat ttccgtcttt tttctttaat gctttaaata
    11101 ttaagaatga tataattggt cctataatag ccattgaaaa agcattagct cctaaagtag
    11161 ttattcctcc atgtgccaat agtaatgctt ggaatattag aactatagtt ccaagaacaa
    11221 acattacact tggtccaaac attattgctc caagacctac tccagtagga tgtgaacaac
    11281 ttcctgttac tgaaggtatc tttagtgctg acaatacaaa tacaaatgct ccacacagcg
    11341 ctaataatac tttcttttta gggtcttgct ttactatttt tcctattgat tttagtccta
    11401 ctaaaaaaaa tggtataaat ataactcccc atgctataga ccattttact ggtaagtatc
    11461 cttccattat gtgcattgca ttcgatgtaa ctggagttaa aacgattaac ataagtgcag
    11521 ctataacccc taatttgata ttttgtttca ttttactatc ccctctctcc ctttctaata
    11581 attaaaatat aaataatata tgtctgattt attttaaaaa taaaaataaa aaagcctaac
    11641 tcgatggcta ggcttctaat tttcgataat ccaaatacaa acacataaaa tgaacatatc
    11701 tgtataatta ttataagaaa ctttctccat ccccgaagaa attactttaa tattttaggc
    11761 aggtctcctg acttagcttc ttcctactcc tcttccttcc catgagatat aaaaaatatc
    11821 atcatagtgg ctaacaaaag tttcgtctgc atcacagtag cgggggctgt aaaggaattt
    11881 aacctttttc cctattaatc ttgcttaaag aacctataat attatatatt taatttttaa
    11941 attattctta aatcattttc tatataaatc attgtatacc ttttctgtga aaatttctac
    12001 attttttata aattttattc atttttatat ttttatgtat ataaattctt ttttgtaagt
    12061 ttattaattt ctaattatct tctttaaata cattgaaact acatcagaaa atctagttat
    12121 atctcttgaa tatataaaat catttccata tattaatctt tcagcttcaa gttctgattc
    12181 ttttcctgta aatactccta gtactaatat attttgcttt ctagcttttc tcacttcatt
    12241 ggctgtatct ctcaaaccaa ctatacccct ataagccttt tctcctctta atgttctctc
    12301 actacttttt cctatcttta catcatttgg tcttccatcg ctgagaacta tcaggatttt
    12361 attttcttca tctctattta ttagaccact acatatagat tttattgcca aaccatctct
    12421 attattacca gcacaaaaat attcaaatat attttcacta ttttttatac tatcatcata
    12481 atctttaaat ctttttaata ttgtatagtc taaaaaacta gaaaatccca tcactctatt
    12541 tggtatgcca acttctgtta gagcatttgc aattatgtaa gcttgaattg ccacatttcc
    12601 ctgatttcta ctttgagagc cacttgcatc caataatata tcaataacgt atttcccttt
    12661 ttcattttct atatctttat aaaatatttt attattatta cttctaccaa ctctccaagc
    12721 tctatttgct aatatagtac cacaatcaga atatactcta cttttagcat tttcttctat
    12781 taaaattctt gacagacttt cttttaatct ctttatttgc tgtctataca ttctagcatg
    12841 ttctctaaat tttcctaaat tattttcttt ttgtcttgtt acatatttta gttgaaatac
    12901 attattacac tcacttctaa gaactccatc tgtaaagtgt attctacaac cttcatgaac
    12961 atttctacaa ttttgtgtct caattttttt aatttcacct ttactcaaat aagtttttcc
    13021 ataataatgt tctatttttg aatatatctt atttgcagtg tcttcatcaa catatataac
    13081 tcggtctgct gaattgctac atttagaatc atcaaaatcc cctacacctt ctacaagcat
    13141 ggatgaagat attgaatcta tatcactttc aataatattt tcttcttcat cactataaag
    13201 ttcttcatac atgaaatcag caaatgtatc aaaatttact tctacacttt ctacattatt
    13261 ttcatcaata agactatttt cttctccatt taatatgtat tcgaaataag ttaaatatat
    13321 aacatctaat gcttttaaaa tctccatcat atcttgattg gtatctatat ttcttatatc
    13381 attaactatt cttctagtca tactatctaa tgctgggtgc ttgtccatat tataaagtac
    13441 aaatgtgtat ttaattttat ccataatatt atcattgtgt attttggtaa aacgtgaaac
    13501 aatatcttta taagcatttc ttcttatgtc ttctatccct ggcctatctt taatgacctt
    13561 ttcttcaact attgtattta atataacttg aattagaccc agtataatat ctttgttgta
    13621 tccttttctt attctatttg taagatattt tttaactata tcccaatcta catactttct
    13681 tctagcacct gcaattattg caaaatatag acctatttct tttgaagagt aatctttttc
    13741 tgatatatct atactttcat tgtaatcacc agctattgtc catgataagt tattagctct
    13801 attttcaaat tcataatcat catatatcca tttcataatc tcacctatat taaatatgac
    13861 ttattggaaa tacatctata ctctcccatt tattaggtat tctagtttta attacatctc
    13921 caaccatttc tttctcatat acatcaaaag tctttcctgt aagtcccata ttaattgcaa
    13981 gagttggttt taatcctctt cttatagtct taagagatgc cataagacca cgtaaatcaa
    14041 tagctttact agatatctct ccattttgag acttcaattg taaatctaaa aatattcctg
    14101 caaagtgtat taatttttct tcatcagcat ctgaaaattc attttttaaa atcatcatta
    14161 acttatcttc ttctatagga ggaatatcta ttaccataaa tctagatacc agtgcttcat
    14221 taagttcttt tgttcctgca tattcataat tcatagtccc aataaatctt gttgctggat
    14281 gtagattgac tctctcatac ccaggtacat caattattct tctatagtct aaagctgaat
    14341 gtaccacaac tatagcatca ttttttgcca tattaatttc atcaaatact ccaaaacctc
    14401 cattaatagc acactcataa actgagcctc ttctaagttt aacttcattg tctataaatg
    14461 tatctgtacc tataagtgtt gaactgtctg tatttatatg aaatgatgta ttccactgag
    14521 gtctattaaa tatttcccct agattatcag caagtaaatt ttttcccgtt gcctttggtc
    14581 ccgataataa tatattttct tcttctaaaa tagctgctat acacatagac aagatatctt
    14641 taccataaaa ataagtttta gactttgtta ctctgtattc taaatctttt tctaaaccat
    14701 aataatttct aaaatacagt acatcttcaa taagtttttc atctacccct tgcaccctta
    14761 gattatctat tatatccata tttttacccc ttttatataa atagtatttg tttcatttct
    14821 tatcaccatt ttagcataaa aggatataag ctaaaattga ttttttaatc tcccccattt
    14881 taagtaaaac catgcaccat tttatcacta caaaattaag agtactactt actgatattt
    14941 cttgattttg ttcgtctata cgtatataat atatgctgat aaattatgag gaaaggatga
    15001 ttgaaatatg gaatatatgt cttgcccaga agtagcaaaa aaatggggca tttctgaaag
    15061 aagagtacaa aaactatgtg aagaaagtcg cataccagga gtttcaaaac tcgggtatat
    15121 gtggctgata ccaaagaacg cagaaaagcc tgttgatggt agagcgaaaa gaagaaagga
    15181 agtacaatat gaatagcgtt ttgattatag atgatgacaa agaactttgt gctcttatga
    15241 aaaaatgtgt tgagcaagaa aatttatctg ctattactgc acattgtggt gcagaaggtc
    15301 tacatttgct cgataaaaat agacacacac tctctcttgt aattttagac ataatgatgc
    15361 cagatataga cggttttcaa gttctgaaaa aaattagaga aacaagtaat attccagtaa
    15421 ttatgcttac tgctaaaaat gacgaggatg ataaagtttc cggactacgt ttaggtgctg
    15481 acgattacct aacaaaaccg ttcagcataa acgaacttat ggctcgtgtc aattctctta
    15541 ttcgtcggta tactatgtta aaccctgctt ttataacaga cacagattgc ataataataa
    15601 atggcatgat tattgacaag acaaaccgaa ttgtttctat caataatatt cctgttcaac
    15661 ttacaagcaa agaatttgat ttacttgctt ttctggcttc taataaggga cgggtattta
    15721 ccaaaaaaca gatttataca caagtatggg aagaagaata tgcttttgac gatagtaaca
    15781 tcatgtcttt catcagtaaa ttgagaaaaa agattgaacc agaccccaac catccatttt
    15841 atattttgac agttcgtggt gtcggttatc gttttaataa ggaggcttaa catgaataca
    15901 agtgtctatc tacttttgat attggctctt agcctgatta ttatagcgta ccttgtttct
    15961 gtcctgctac gtgtccgaat acaacttaca cttataaaag atgctttaga ggatataaaa
    16021 aatggaaacc ttaatcgccg tattttaaca gatgaaaacg acattacaaa acaaatttgc
    16081 tatgatatta acgaaatagc aatcaatagc caatttcaac ttattcagca aaaacaatca
    16141 gaacaagcct acaaacagct aatgacaagt ctctcccatg atgtaaaaac tccacttgct
    16201 tctttggtcg gttatttgga agcagtagaa agtaaaattg ttgtaggaga ggaaaaagac
    16261 gagtatatcc atgttgcgtc taataaggct cattacttaa agaattttgt agaaaatcta
    16321 tttgaatggg taaaattgga ttctaaggaa caagtttttc attttgatat ttttgacttg
    16381 aatgaattat cccgtaatat tatatctgat tggatacctg ttttagaaag cagtcatttt
    16441 gaatatgaat ttgatatacc agaaatagag tacttcttac gtatagacgc aaatgcatat
    16501 acccgtatca ttaataacct attacaaaat attattaccc atagtagtgg caacaaaatg
    16561 acattgagaa tttttgaaaa caaagagcaa gctcaaatta taataacaga taatgggaaa
    16621 ggtatatctt cagacaatct cccacatatt tttgaaaggt tgtaccaatg cgaccattca
    16681 cgagcttcaa agggaaatgg tttaggcttg gcgattgcaa aggaacttat taacgctcat
    16741 aagggaaata ttaccgccaa cagcactccc ggtatgggaa cagaatttac aatattgctt
    16801 cctaaagccc tgtaaaagta cgaggctttc ttactctaaa acaagataaa agcaagatta
    16861 tggcaaggtt ttggcaaggt tcatgtttta taattctaat tgtagttgat tttgacgttg
    16921 tatcaactat cttttgtaga aacaaattta ttaaaagtga ggaaattata tatgaatgat
    16981 tttgtgattg aaacaaagca acttacaaaa atttatggga atcaaacagt agttgacaaa
    17041 gtcaatctcc atgtaaaaaa aggccgaata tatggtctgt taggacgcaa cggagctggg
    17101 aaaaccacaa ttatgaaaat gattttagga ttgacttcaa tcacgtatgg cgaggtagat
    17161 gtatttggag agaatattaa gggatgtgaa aaacgagtat atccccgtat cggtgcaatt
    17221 atcgaaacgc caggatttta tcctaatctg acaggaacgg agaacttgga gatttttgca
    17281 aaacttcgtg gtacagccgc ccctaattct gtaaaaagag cattggagat tgtaggctta
    17341 ccttataaaa ataaaaagct gtttagtaaa tattcacttg gcatgaaaca gcgacttggt
    17401 attgccaacg ctatcctgca tgacccggaa ctgttagttt tagatgagcc aaccaatgga
    17461 cttgacccta ttggtattgc ggagatgaga aagtttatta gggacttgag cgttgagcat
    17521 ggaaagacaa ttcttatctc cagtcatatt ctttcagaaa tctcattgtt ggctgatgat
    17581 attggaatta tcgaccacgg tgttcttttg gaagaaagta gtatgagtga actagaaaag
    17641 aaaaacagta aatatatcct gctacaagtt tcagatattc caaagacatc acttatttta
    17701 gagcgtcaat ttaacacgaa ggattattcc gttcaagatg accacacttt acgcctttat
    17761 aacactactc tggatatggg tgaaatcaat aaagcacttg taatgcagaa tgtaactgtt
    17821 attagttctc aactttgtaa tgatacttta gaggactact ttaaaaaaat cacaggagga
    17881 gagggcattg cttaaactta ttcaaacaga atttcttaaa ctgcgtcgta ggaaacttat
    17941 ttggctgatg ttgttgtctg ctctcattat gccgtttttc gcactactat acttcaatta
    18001 ttttggtaaa acaggtgttg agccaacaaa gttttataaa tggtctgcat ttggttatac
    18061 actttggatt attttgcccg ttgttttggg tatattttgc accatgctta tacacgaaga
    18121 aaaccagtat gatatgctca aacagctttg gattgtacct attagtaaaa tgggatactt
    18181 ttttagtaaa tttttcgttg ttttgattta ctctatttgt tttatgctga ttacagccgt
    18241 tgcttctgtg ttatttagtg tactttctgg atatgtcgta tttagctggg caagcatttt
    18301 atatttgttg aaaaaatgtt tagaaattgg tgtaatcact gcttttgtaa tgttgccgat
    18361 tttggcaatc gctacatctc aaaaaggata cattctccca atttgtatca cattggtata
    18421 tgccttttcc ggttttgtta taatgacaat aaatatgtat ttacacccat tatccagtat
    18481 gggagttatt atcatgagaa ataaagacat tccaggtctt atattttcac aggaaattaa
    18541 tgtcccatta gcatttcttt gtatttgtat ttggagtatt gtatcagtat tgtttgcaag
    18601 cattgcacta ggaagaagaa agtgaggtgt agataatgtg gaaattactt tgggctgaat
    18661 gtcaaaaaat tcgtcgttca aaaattgttt ggattacatt ttttgcatcc attatgatag
    18721 cagtcatagt ttttgcagga gggcaggatg tatataatga cccagattta cattatggct
    18781 taaagagcgt ttatgacggt tcacggtata tagacaatgc aggttggtat atggacgcgg
    18841 tacaaccttg gtcaactttt tttgttcttc ctgctgtcat tgccctatta ggaagctata
    18901 ttatttgtcg tgaagaagag gaggacacta ttaaatccct gcgtcttatt cccataaatg
    18961 aaacaaaatt aaccatagca aaaatgatta ttacttttat attcagtata cttctttatt
    19021 tgttactctt tactataaca tttttgactg aatccgtttt acatttttcc gatttatcga
    19081 caaaactggt tttaagttgc atgaaagagt attttcttga tggcataggt gtattctttg
    19141 caatttctcc cattattgcc ttggtatcgc gcatgaaaaa aggatattgg cttgcattag
    19201 tattcacaga agtttattct attgctggac tatttgcggg tatgtcaaat gtcttacaaa
    19261 ccttttttcc tattgcagca atatttaata tttctggata ctatataact actggagaaa
    19321 aaattatggg gagtatcata agtttattac tttgtgcctg cctttctgca tttatactga
    19381 aagggctaaa acacaatgaa aaaaattgaa ataggtatat caaaatgaaa aagataaaaa
    19441 aactgattat tctaattgta ttcttgtttc tttttggcat gttacactgt tatactgaat
    19501 ggaaattcaa caatgaccca cctaaagggg taatagaaag aataaatgag tggactgata
    19561 agattggact ggattagtaa cttatgttaa ttatagtgaa tgtaatatta gcaagttatt
    19621 gtttcgtatt ttaatactat aactttaaat atttggtttt aatcttccga acgaatataa
    19681 aaaaactgtc acagaggcaa catatttttg aatatttata aagtggtagt tttgattttc
    19741 aaagctgccg ccttttcttc tatataagca aacttatcct caacaaagtg taaaacatgg
    19801 tacattttaa gaaaaaagta ccacagacac aagcaataaa agaaacgccg agaacctaca
    19861 aaaacgtagt gcttatgcag atttacgacg ccttctattt tctcaaccgt cttaatgacc
    19921 ttgtacaaaa gaaatttttc taatattttt tataatctta tcttgtgatg ttttttatgt
    19981 ataaatgact taataacaaa tccaataaaa attatggata ttggtataat tagaacgagg
    20041 aatttataat cattataatg tgacattatt cttacccaac tctcagaaaa aatatatcct
    20101 atactgatta atgaagtatt ccatatggtt attcccacga tagatgatat tacaaaagtt
    20161 ataatatttt gacctgctat acctgctata aaagatatgt atgttctaca taaagggatt
    20221 aatctacata cacaaacaga aatagaagag tatttattaa aataatcttg agatgcaaat
    20281 attcctttct ttgcctttgg acatatctct attattttat ttatgataga tttacctccc
    20341 caatttccca ctgtataaca aaatatcgaa ccaagtatac ctgcaattat acttaaaatt
    20401 aaaattgtca gaaatccaaa atgatttctt gcagcaattg ctccacataa aggtaaaaca
    20461 acttcgctag gtaatggaaa acaagcatac tcaatcataa ttaatacaaa tacagagatt
    20521 aatccatatt gtaaaataaa actattgatt acagacactt acttatctat ctcctttctt
    20581 atttaaaggt tttaagggta catattctaa ggattctcac cctatattta aagaatcaat
    20641 ctctttaaac tattatatgt atttacacaa agcatttata acatacttta aaaatagtac
    20701 cttaaaataa gaaacttaat aaataaaaaa agattacgtg gctacgtcct actctcccag
    20761 gcag
//
LOCUS       DAMKPG010000274        15440 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.091, whole genome shotgun sequence.
ACCESSION   DAMKPG010000274 DAMKPG010000000
VERSION     DAMKPG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.091"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            249..926
                     /locus_tag="PC621_003941"
     CDS             249..926
                     /locus_tag="PC621_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4110999.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            998..3013
                     /locus_tag="PC621_003942"
     CDS             998..3013
                     /locus_tag="PC621_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF4111000.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3063..3743
                     /locus_tag="PC621_003943"
     CDS             3063..3743
                     /locus_tag="PC621_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4111001.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            4125..4823
                     /gene="vanR"
                     /locus_tag="PC621_003944"
     CDS             4125..4823
                     /gene="vanR"
                     /locus_tag="PC621_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HDF4111002.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            4859..5974
                     /locus_tag="PC621_003945"
     CDS             4859..5974
                     /locus_tag="PC621_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4111003.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            6119..7006
                     /locus_tag="PC621_003946"
     CDS             6119..7006
                     /locus_tag="PC621_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4111004.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            7007..8242
                     /locus_tag="PC621_003947"
     CDS             7007..8242
                     /locus_tag="PC621_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4111005.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            8624..9541
                     /gene="pstC"
                     /locus_tag="PC621_003948"
     CDS             8624..9541
                     /gene="pstC"
                     /locus_tag="PC621_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF4111006.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            9543..10367
                     /gene="pstA"
                     /locus_tag="PC621_003949"
     CDS             9543..10367
                     /gene="pstA"
                     /locus_tag="PC621_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF4111007.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            10386..11150
                     /gene="pstB"
                     /locus_tag="PC621_003950"
     CDS             10386..11150
                     /gene="pstB"
                     /locus_tag="PC621_003950"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF4111008.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            11178..11831
                     /gene="phoU"
                     /locus_tag="PC621_003951"
     CDS             11178..11831
                     /gene="phoU"
                     /locus_tag="PC621_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF4111009.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            11989..12858
                     /locus_tag="PC621_003952"
     CDS             11989..12858
                     /locus_tag="PC621_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF4111010.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            12945..14381
                     /locus_tag="PC621_003953"
     CDS             12945..14381
                     /locus_tag="PC621_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF4111011.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            14429..15256
                     /locus_tag="PC621_003954"
     CDS             14429..15256
                     /locus_tag="PC621_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF4111012.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
BASE COUNT         6039 a         1670 c         2717 g         5014 t
ORIGIN      
        1 tttataagtg aaaatgactt taagctccat tctccaacaa tcggagactt taattagtat
       61 ttaataataa atcttaatgt tcaatacaat attcaagaat tgttcaaaaa tacttaagta
      121 ttatagtcta gtttgtaaag atattataaa ataataatac tagttatata tgaaaataaa
      181 acattataat ttgtaataat tataaaactt gagattatga ttaaaatacc tttgaatgga
      241 gtggtgtaat gtataattta ctggttgtag atgatgatgt tgatatttta aatataaatc
      301 aaatttattt ttctaatata ggttttaatg tatatacatc agagactgct aaagatgcta
      361 tggatatagt agagcgtata gttttagact gtataatttt ggatatttca ttgccagata
      421 tagatggata taaaatgtgc aatgatataa aagaaaaggt taacatacct ataatatttt
      481 tatctaatta tgagcatgaa gaagaaaaag ttcggggatt tttagctggg ggggatgatt
      541 atataacaaa accttatagt ttaaaagaac ttgaacttag aatatatgct cgtatgaggc
      601 agtacaaaaa tattacatca gttcctaatg tgttaaagtt ttcagcgtta gtaataaatg
      661 taaattcaag aaaagtaact tttgatggag aaagtataga tttaactact atggaatttg
      721 aaatattatt gtttctagct gaaaacaaag aacaagtatt ttcgaagata gagatatata
      781 accaagtttg gaaaatgcct gatattggag atcaacatac agtgcaggtt catattgcac
      841 agatgagaaa aaaaattaat tctttaagca gggagcatca atacattcaa accgtatggg
      901 gaaaaggata taagtttgta ccataagtga gatactgtaa ttaataaata gttcttgttt
      961 tatttaatac tactatgaaa gagatgtggt tgatgcaatg aaaaagtata atatactaaa
     1021 aggaactctc attgtattga tagtctctct gttggcaata gcaggattaa atcttctgta
     1081 taaatatgat aataaatata catataagtc tattagagca agtaatggaa ttttaactat
     1141 agataatgat actttcacta aaggtaaact tgtctttctg atagatgact gggagtttta
     1201 taatcataaa atatttaaac cgaatgattt tacagagcaa gatataaaac cagaatatgt
     1261 ttttataggt caatatccag atttttctat gagaaaaggg aatcagtcac catatgggaa
     1321 tgctacttat cgtatgagaa taaaaaatga aggtgaggat aagctactaa gtttagagct
     1381 tcctgagatt ttttccgcaa gtgaagtatg gataaatgga gaaatagttt ctaaattagg
     1441 agatgtagga acagaaaaat atagacccaa aattaaaaat tcagtagtaa gttttattgc
     1501 aaagaaagat acagaaatta taatcaatgt atcgaatttt tcacattatt atagtggttt
     1561 gtattatccg cctgcattag gagaaacaaa ggacataagt aatatgatgt tttatcgatt
     1621 acttttttat agtataattt gcttcacaac ttttgcaata gctatatttt cgttgtctgt
     1681 atggtttctt tcaaacaaaa gtaaacttta cttatatttt ggatgtatgt gtgttttctt
     1741 tgccatccat gtatcctatc cctttgtaca tttgttagga ctgcctttgg taagctttac
     1801 atatgcggtt gaagattcgt cctattttat ggtagtttta tgtattattt gtataaatgg
     1861 aagtatatcc aaaatagaag aagataaagt gtatagattt ttaattttac cattaggaat
     1921 tacaatgagt atcatatcta tagtgatacc aattattctt cttccatatg atatgtactt
     1981 tgtcaacata tatgggaaat taatagacat atataaatat attgtattta tatatatatt
     2041 aatgtcttcg ctggtttcaa tatataaaaa gacatgctct gagcatataa gttcttatat
     2101 tttagtttca gtaaacactg tttttggaat tagcatatta tttgatgcag ttactagtaa
     2161 tagatttgag cctatattta cagggtggca aacagaatat tgtggattta taattgttat
     2221 tttattttct cttatgatga taaacaggaa tagaataata ataaaagaga atgaaaaact
     2281 tacaaggaat cttgaacatg aagttgaagt gagaactaat gaacttacaa cattgttaaa
     2341 tgaacgtaaa aaattcttgg cagatgtggc acatgattta aaggcacctg tttcagctat
     2401 acaatcattt atagacttga taaaggttgg taatattcat gtagatgaag agacaagaac
     2461 ctatctttat gctataaatc aaaagtcaaa tgaagtacaa aatagagtac gtagtttgca
     2521 agaatttaca tcacaagata aaagcattgg tattagtgaa aaaatatgtc taaatgattt
     2581 tattaaagag atatatgaaa ataacttgcc agatacacaa gcatgtggtg taaactttga
     2641 aacttacata ttggagaaaa aaatatatgc aaagataaat aaagagatgc ttacaagagc
     2701 atttgagaat ttaatttata atgctttgac atttactcca tttgatggaa agataatttt
     2761 atcaatggat aaagaagatg gatttgcaat tattaaagtt tctgataatg gaaaaggtat
     2821 acctccagaa aatataccaa aggtatttga cagattcttt acagaaagag ataataatga
     2881 gccaaaaggg caagggctag ggttatatat tgtcaaatcc attataagag agcatggcgg
     2941 agaaatcttt gtagattcag aactaggaaa aggaactgtt tttacaataa aattaagtct
     3001 atgtttttta taataatagc ttatttaata taaaagaata atgatatttg ggagtgatta
     3061 taatgcaaac taagatattg ataatagatg gagacaagga taattgccag aagctaaaag
     3121 gatttctgga ggaaaaagaa ataagtatag atttagcata taattgtgaa gaagctattg
     3181 gaaaaatatt tagcaataaa tatgatttaa tatttttaga aataattcta actgatggtg
     3241 atggatggac tctttgtaaa aaaattagaa atgtaactac ttgtccaatt gtttatatga
     3301 cttatataaa tgaagaacaa agcattttaa atgctttaaa ttctggcgga gatgattact
     3361 taataaagcc tctaaattta gaaatattat atgcaaaagt aaaagctatt ttaagaagaa
     3421 tgaactcata tgtaaataat aatgaaaata atacaaaatt agaggaatat aatagaacta
     3481 gtggagtaat aaagttagaa gacaaaataa taaaattgac tccaacagaa aataagctgt
     3541 taaacttttt tatagagaat cctgaaaaga ctctgactac taaagagata tatgaacatg
     3601 tatggatgaa tgagtactta gaggataact atagtgtggt agttgcagtg aatggattaa
     3661 gaaagaaaat agagaaagat tataaaaatc ctcagaaaat tataacaata agaggagcag
     3721 gatattattt taataaagaa taataaatat attaatctca ttaataattg ttataaatgt
     3781 gaaatgctaa cgatatctaa actaaattat tataaaatag ctgactgtaa tttaattgat
     3841 aaagcctatt cttctagact ttgccagtta aattacagtc agttttatta tatgaaaaaa
     3901 tgcgtaacag aactattatt ttatagatta tatttgaaaa gatagataaa ttgtatatct
     3961 aaatctatat atattcattt ttatctttaa tatatgttaa ataataaaat agttacataa
     4021 taagattaag aatttcttaa taaattctaa tgtaaaaaat catataaaaa aattaaattt
     4081 agtataatat atataaatat atatagaaag gatgtttaaa gtctatgagt ggaaaaattt
     4141 tagttgtaga tgacgaacgt gatatagctg atttaataga agtctacctt acaaatgaaa
     4201 actatattgt ttataaattt tattctggtc ttgatgcact aaactgcatt aatttaaatg
     4261 aatttgacct tgcaatttta gatgttatgt tgccaggagt aaatggattt tctttatgcc
     4321 aaaaaatccg agaaaaatat acttatccaa tcattatgtt aacagctaag gatgaagaaa
     4381 cagacaagat tacaggctta actttaggtg cagatgatta tgttactaaa cctttccgac
     4441 ctttggaact tgttgctaga gtgaaggcac agattagaag atataagaaa tacagtggaa
     4501 gtttacaatc tggtgaagaa ctttcacata ttttttcttt tttaggtctg gaattgaata
     4561 cccaaacata tgaatgccat ttaaatggta aagctctttc acttactcca acagagtttt
     4621 ctattctaca tgtattattg aaaaataaag gctctgtggt aaattcagaa aatctttttc
     4681 atgctatctg gaaagatgaa tattacaata aaagtaacaa tactataaca gttcatattc
     4741 gccatcttcg tgaaaaatta ggtgatactg tagatgaagc aaaatatata aaaacaattt
     4801 ggggtgttgg ttacaagatt tgacacctat ttttcttgtc aagaagggat gtagtattat
     4861 gaaaaagatt aataatttta cttataggaa actaagagat aaatataaat ttataaaaag
     4921 agaaaaaaat acagaggcgt atttaaagta taaattatat ataatgttag ctatctatac
     4981 aggtatagta ttggttttaa tgtttatggc tgattatacg ttttcaaatt tttcaaatcc
     5041 tcttgttaaa tggcttaagt tgagaagaga tgtattattt tttttggttt ttataattgg
     5101 atatatcact attttcaatt tttattggaa aaaaccatgg aattatttaa gagaaatcat
     5161 caatgcaaca catactattt atcaacaaaa tgatgaattg gttgaactat ctaagccatt
     5221 aaaggaaata gagcaacaaa tgaaccaaat taagatgtct gttttgttaa gtcaacaagc
     5281 agtgaaagag gctgaaaata aaaagaataa ccttgtgatg tatctagcac atgatataag
     5341 gacaccactt acttcagtta ttgggtattt gagcttatta aaagaaatac ctaatatgga
     5401 taattctcaa aaatctaagt atactgaagt tgcacttgac aaagcacaat atttagaaaa
     5461 acttatcaat gaactttttg aaataacacg atataattct aatcaaatca aaattaattt
     5521 cgaaaaagta gacctatatt atatgttact tcaattaaag gatgaatttt acccattgct
     5581 gtctgaaaaa ggaaatagag ccattctaaa agcaaatgaa agtttaacaa taaaagcaga
     5641 ttctgaaaaa cttgcaagag tgtttaataa tatcttgaaa aatgctattg cttacagcta
     5701 tcctaataca gaaattatta tctcagcggt tcataaagac agcaaggtag atattgcttt
     5761 tagaaattat ggacagacaa ttccagaaga acaactttct gctatatttg aaaagttcaa
     5821 tcgtttagat gaagctagaa aatctgatat gggtggtgca ggtttagggc tttctatagc
     5881 taaggaaatt ataaatttgc atggtggtga tattactgtt caaagtaaag atgaagtcac
     5941 aacatttact atatcgcttc ctacatctat ttagtaaaat ataaatcaaa tattaggatt
     6001 ttcttaagaa attatctatt taatattaaa attatgagtg tctttgttta gtaaaataaa
     6061 tactaagcaa gacacttttg tttttgcagg taagtagata aagagaggag tttcaaatat
     6121 ggaattaaaa attgaacatg tgacaaaaaa atttaaggat aaaattgctg tagaagatgt
     6181 ttgccttacc ttaacaccag gtgtgtgggg atttctagga gcaaacggag caggtaaaac
     6241 taccttgatg agaattatta cagacattat gaaacctacc aatggcaaaa ttctgtataa
     6301 tggaaaaaat atttatataa tgggtgctga gtacagaaat ttactgggat ttttaccaca
     6361 agaatttggt ttcccacagg atttttctgt aaaagattat ttagaatata tagcagcatt
     6421 gaaagatgta ccaccaagag aagtaaaagg aaaaattaat aatctattgg aaattcttac
     6481 attatctgat gtaaaaaata aaaagattat taaattatct ggtggaatga aacgacgagt
     6541 gggaattgca caagccatgt taaatgaccc taaaatcctt ataatggatg aaccaacagc
     6601 agggcttgac cctggtgagc gagtgagatt tcgtaatttt atttcagaat tttctcataa
     6661 tcgtgttgtc ctaatatcta ctcacattgt atctgatatt gagtacatat caactcaaaa
     6721 tgctattatg aaaagtggaa aaattcttga tgtgggaaca accgataaac tggttaagaa
     6781 agtagagggc aaagtatggg aatgtcttat atcaaataga gatatttcaa aatatgaaat
     6841 gaatttacgt ataatcaatc agcgtagtga aaaaaacaat caagtatcta ttcgttactt
     6901 ggcagaaaag tcacaaattg ataactctat tgctgttgaa ccacgactag aagatttata
     6961 tttatggtta tttccagaag cagtttatag taaggagggg aaataaatgc gtatttttta
     7021 tttggaaatt aaacgagtgt tgcactcacg tcgtacatta attttattat tgttggcatt
     7081 ggttttatct gcattgatgg cttttcttcc aattacttac gagggtatca attatttaga
     7141 taataatggc aaggtagttg aattgagtgg tttagatgct attaaatata aaaaaacaat
     7201 ttatgctaat aatagtgaaa ttagtagtgg agttgtaaca ccaaaaaaat taaagcatgc
     7261 tttaaatata tggaaaaaaa cagaagaaaa gtatggtaaa gtaggtagtg aagatttccc
     7321 actagatatt tattctaaaa gtattttacc aatacgccca ttgttgaata gacttcctga
     7381 agcatttgct aattcagaaa caggatttgc aacagaatta tcagaaataa atacaaatga
     7441 tttagattct ttttatgaac aatgcaaaac tcatttaaat gatattatga agattgaacg
     7501 acaaacttcc agtgcacaaa tgaaagctca agaactttat agaaaagtag atactccatt
     7561 tgagatatac ccaggttatt ctaaagatgc tttcgattat ataatatttt acatttttat
     7621 attattaatt atatgtgttg caattactgc acctactttt tctggtgagt atcagacaca
     7681 atcagatagc atccttcgtt gtacgaaaca tggacatact cgtctagcgt ttactaaaat
     7741 attagctcta ttttctatat ttatagtaat gtttgttgta tgtatttcta tccatttggc
     7801 aatttctgat ttagcttttg gtactgaatg tttgaaaacc tcatttcaaa tgttgttttc
     7861 tgtcattagt ctagtaaata taaatttact acaagcacag attcttatag ctattggtgg
     7921 tctaatttca atacttgcaa tggttagttt aacattattt ttatctgcaa aatgtaagga
     7981 ttctcttact tctatgctga ttgctttttc tatttgctta attcctatgg gaataggtgt
     8041 tagttggatt ggatacctat taccaccagg gggaattggt cttaagatta gtttattata
     8101 tcaaatgaca tcatttaatt tcttacactt aggaggtatg agtttttgga caccacacat
     8161 tatactgttt ttctcagtga tagaaatacc tatttttata tttttggcta ttcgtaccta
     8221 ttgcaaacat caagtatcat aacaatttga tagaaataat agtgatataa actagaggat
     8281 acgataatta tatatgtagt ataaaagtta aatttagtta acaaatctta atagcagata
     8341 taggtaataa attatacata aactgtatta ttatctatat ctgctatttt aatatagaga
     8401 aacaacagta tattgtaaag taaatgtcaa atatagtttt aaaagtaaaa ttaatgttaa
     8461 aaaagtaaat ataattttac acttgcttaa cttaatccta atatagattt aacttaaaaa
     8521 aagtagaata aaagtacagg aaatttgatg aagacgaatt ataaaaaatt aaattttgaa
     8581 tacagaacga aaatcgataa gtatatttta atgggagggt attatgatag taaaatctag
     8641 attagtaggt ttagaaaaaa atgacaataa tggcaataag acaaaatacg taattgaaaa
     8701 agtagcaaaa aatatttttc tacttagtgc tttagtagca gttgcaagtt tacttttgat
     8761 aataggattc gtattttata aaggcttaag accatttata tttgaaggat attcatttat
     8821 agacttttta actggtgcag attgggttcc ttctgccaat aaatttggaa tatcatctat
     8881 gatagtagca tcaatatttg caacacttgg agcacttgtg ataggagtac ctgttggtat
     8941 attaacctct gtatttatag cagaagtagc acctaaaaga ttagcaaaaa taatgtcacc
     9001 agcagttgaa cttttagcag gtataccttc agtattatat ggtgtatttg gtcttgcagt
     9061 gatagttcca actatacaaa gtatatttaa tcttccaaaa ggtcaaagtt tgttagcaat
     9121 agtaatagta ttagcagtaa tgatgttacc aacaataata tctgtatcag aaacagcaat
     9181 aagagcagta ccaaaagcat acaaagaagg ttcattagca cttggtgcat caaaaataga
     9241 aacaatattc aaagttgtac tacctgctgc gaaatctgga gtattagctg cagtagtatt
     9301 aggagtagga agagctttag gtgaaacaat ggcagtaata cttgttgcag gaaactcacc
     9361 agttatgcca actgcaataa cagatagtgt aagaccactt acaactaata tagcacttga
     9421 aatgggatac gcatttggta cacatcaaga aatgttattt gcaactggag tagtattgtt
     9481 tacatttata ttaattttaa acttagtttt aaataaactt tcaaataagg cggtaaatta
     9541 agatgagaaa gttcaaagag aatctattga agtcattagt atatctatca gcactattta
     9601 caatagtatc attagtcatt atagtgggat ttatatttat aaagggaatt ggcaacatga
     9661 attttaattt cttatttagc aattattcag catcaggtga tggtggtata cttccaatga
     9721 taattacaac attatataca gtagtagttt ctatagttgt agcaactcct ataggaatat
     9781 tggctgctat atatttacaa gaatatgcta aaaaaggaaa agcagttaca gctataagat
     9841 ttgcaactga aagtttatca gggataccat ctatagtata tggattattt ggaggaattt
     9901 tctttgtagt tacattaaaa atgggatatt ccatagttgc aggttcactt acagttgcaa
     9961 taataatatt accagtaata attcgtacaa cagaagaagc tttaaaaaca gttcctcaag
    10021 cttatagaga atcatcattg gcattaggag ctacaaaatt tcaaacttta tataaggtag
    10081 tacttccaag tgcaatacca ggaatcttat ctggagtaat cttatcagtt ggtcgtataa
    10141 ttggagaatc agctgcaata ttacttacag caggtactgt agccaagatg ccaggtggaa
    10201 tatttgatag tgcaagaaca ttaactgtac actcatactt actgactaaa gagagtggag
    10261 atatagcaac agcagccagt ataggaattg ttttaatagt aattgtatta gcactaaaca
    10321 tgctagcaag atttgttgct aagaaattaa ataaagctaa ttattaaatt taagaggtgt
    10381 gaataatgga attaatcgat aagataaaaa tgagtgtaaa agatttggat ttattttatg
    10441 gtgataagca agcattaaag aaaataaata tggatataaa agagaataag gttactgctt
    10501 taataggtcc atctggatgc ggaaaatcaa cttttataag aacgttaaat agaatgaatg
    10561 acttaatcga agatgtaaca attaaaggaa acatttctgt tgatggagag gacatatata
    10621 cctcagatga tgttataaat cttcgtacta aagtaggaat ggtatttcaa aaaccaaatc
    10681 catttcctat gagtatatat gataatgtgg catatggtcc tcgtacacat ggactaagag
    10741 ataagaagca attagataaa attgtagaag aaagcttaaa aggtgcagct atttgggatg
    10801 aggttaaaga caggcttaaa tcatctgcac taggactttc tggtggtcaa caacagcgta
    10861 tatgtatagc aagagcaatt gctatgagac cagaagttat tcttatggat gaacctacat
    10921 ctgctttaga cccaatttca acattaaaag tagaggaact tatagaggat ttaaaaaaag
    10981 actacacaat agtcatagtt acacataata tgcaacaagc tgctcgtata tcagatgaaa
    11041 cagctttttt cttaaatgga gaggttatag aatttagcga tacaaaaact atgtttacca
    11101 ctcctgtaga taaaagaaca gaagactata taacaggtag atttggttaa aatatataac
    11161 tataaaggag ttagacaatg gtcaataata gtttagatat aagtattgat actttaaaag
    11221 aacaaacagt acttatgatg gaaaaatgtg aagaagcaat agaaaagtct gtgacttgta
    11281 tgatagagaa ggatttagag ggtgcaagaa aagtagtaga agatgatgaa gaaataaata
    11341 aattaagaga agaaataaga gataagagta tagagctaat agtattaaag caaccaatgg
    11401 caagggactt aagatttatt tattcattag gtaatatatc gatagagttg gaaagaatag
    11461 gagattatgc agcaaatata gctagagaaa tgttgaagat aggtggagag gcttatataa
    11521 aagagctgat agatattcct aagatggcaa aagaatgtaa gtctatgatg tctcagacta
    11581 gagaagcttt aatcttattt gatgcaagta tagcttatga tgttggaact aaagatgata
    11641 ttgtagatgg attgtatgaa caagtacaaa ttgattgttt aaaaataatg catgaaaatt
    11701 caaatactat agaccaagga gtaaaacttc tacttatagg aagatacctt gaaagaattg
    11761 gagaccatat aacaaatgta tgtgagaaga taatttttgc aattgagggt aaaatggttg
    11821 agataggata aaaaactgct ttaaagcagt ttttttattg tctaaattaa gaaagaattg
    11881 ataaaaaaat atgattaatg ggtaaattta aaaaattgtg atatactagg aacatattaa
    11941 atatgaggat gcgtatttta tataaggtgc tttttaggag gtggaacttt gaaacttcaa
    12001 aagatgggta tgaggactat aaaaacagga attagtgtca ctttatgtat gattcttggt
    12061 aactatctag tagagaatat gttttattct ctagtagcat gtataatttc tgttcaagat
    12121 acggtaaaag gttctttaaa gacaggaatc aatagagtgg aaggtactat tttaggtggt
    12181 ataataggat tcatatttgc catcattaaa gctggagacc cttttttatg tggtctagga
    12241 gtaatgtgct caatatattt atgcaatctg tttaaaataa agtcagtagt agttgcctgt
    12301 gtaacctttc tggctataca tctaggtgta ggaaatagtg accctgtgta ttattcttta
    12361 catagagtgt tagatacatc tgtaggtgtt ttgtttggag tacttgttaa ttattatgtg
    12421 gctagaccaa attatctaaa tagtgttata gatgaattta aaaagataga agaactttca
    12481 atgttactta ttgaagataa aattcttaga aaagaaaatt taaatgtaga gaaatttgaa
    12541 gaagaagtag aaaaattaga agaaatatat tcaaaactta ttgatgagtt ggattatagt
    12601 ttgagtgaaa ctaatataga aaaaattgag aatgccttaa ggatatgtag agaaatacat
    12661 tttcatatgc agtctataga gttacttgaa aataaacttt atttgaaaga gcatacatat
    12721 aaatacctta agaaaacata taatgtggat aagctagact ggaatataga tgaagaaaaa
    12781 agccccgtat tcaactatca tctaatgaaa ataatgattg aaatagagtt gttgagaaag
    12841 ctaaataaat catattaata atagtagtta aataataaat aaaaaatagt atctttgtaa
    12901 atattaaaca tatataagtt taatttatgg aggtactatt ttttatgaat actagaaatg
    12961 acaattctaa aatactagaa atatataaaa tgaagctttt tggcgaactt gtaaaagcag
    13021 tgtggaatga tgatatagag gatattgaaa acttacctaa taaaatttta aatgaagatt
    13081 taaaggataa tttaaataat tattttagtg gaaaatttga taaagatact atggtaaatt
    13141 taattagggt aactatggga ttggatgtga cagaagatac agataaaaat ttaaaagagt
    13201 cactacatga agctgtaaat ttagatgaag tcaaaagacc aataatatct gtaatatcag
    13261 atgcttgcaa atattgtgat gaaaataata aggaagatga gtcatgtaag ataagaagta
    13321 agcatattaa ttgtaatgag gaagatgtat gtagtgcttg tggtgattgt gtatcaagat
    13381 gtaagttggg tgcaatttca gataaaatag aatttatacc aatgataaaa ttgttaaaag
    13441 atataaagag ccctgtatat gcaatagttg caccagcttt tgtaggacag tttgggaaag
    13501 atgtaagccc tggtaaatta agaactgcat taaaaaagat aggatttgaa gatatgatag
    13561 aagttgcttt ggcagcagat atgcttactg caaaagaagc atatgattat tatgagcata
    13621 tgaaaaagga cgaagaagga tgctttataa ctagttgttg ctgtcctatt tgggttagtt
    13681 tgatacaaaa tagttttcct cagatattgg agaatgtatc accatccgtt tctcctatga
    13741 ttgcttgtgg gagagctgta aaaattttaa atccagatgc taaagttgta ttcataggtc
    13801 cttgtatggc aaaaaagaaa gaagcaatgt tagaagatat aaaagatgca gttgattttg
    13861 tgcttacatt taaagagttg caagaaatat ttgatgcatt agagttaaat ccagcaataa
    13921 tggatgagag taatagagta gaggcgtccc tttcaggtag agtttatgct agaacaggag
    13981 gtgtaagtaa agcagttaag ttatcagtca gaagaattga caaaaatata aaatttgaag
    14041 aaaaggcatt tgaaggaaca aaagagtgca tggacggtct aaagaaagtt ctaaataaag
    14101 aaatagaagc tacatttata gaaggaatgg ggtgtgtagg aggctgtgta ggaggtccga
    14161 agcgtatttt aagtgtggaa gaaggtacta agaatgttga tgaatatagt gaaagtacga
    14221 atatggaaac tccatttgat aatttaaatg tattacaatt tttaacttct ttgggaatca
    14281 aaagagtaga atctttagga gaaaaagaag aagaaaaggt taaaaaaata tttagtagag
    14341 atataaaaag taataagaaa gagaataaat ataaaaaata actttttaag tcttaaactt
    14401 atgcttttat aaaagggaga tatgggggat gaaaattaaa gataataagg ctgtttttca
    14461 tgtatttata aggatatttc ttatagctgt tttaatttta actggaacat tattggagga
    14521 aaaaattaat aatgttccag ttgaaaatat aagttcaaaa ggtgaaaata cactggaact
    14581 gctttctcaa agtaatactt taggagaaat catatacaaa gcatctcaag aagaaacaaa
    14641 aggtccaggg gctacttctg aaaaagtagc atacataaca atagatgatg gtccatcaaa
    14701 atatacaaat gcactacttg atattttaaa agaaaatgat gtcaaagcga cattttttat
    14761 gcttaatcaa aatatgaaaa atcacaagga agaagtaagg agagtcttgg aagaggaaaa
    14821 ctcaataggt tttcatagtg taagtcatga tatacataag ttatataaaa gcccagaagt
    14881 aacagttggg gaatttaata catgcaataa tactctacaa gaaataactg gtcagagttc
    14941 aaaattaata agattaccat acggaagtaa accatatatg ccagaaggtt catataataa
    15001 acttatggca aatgattatt taatttggga ctggaatttg gatacacagg attggaagtc
    15061 ttctacaagt cagatagtaa gtaatatatt atattatgga agaaaaagag atgaattagt
    15121 agtattaatt catgagaaag agcaaagttt aaatgcttta aataatatta taagaatctt
    15181 aaaagagaga ggatatacaa tattgccaat tacagaaaat ataacaccta aaaatttctg
    15241 gcaagaaaac ttataataaa tttaaatata gtaaaagata aagtaaatta tttataaaat
    15301 aaaacatata atataaaaaa tacgaataag aggtatactg taaggataat ataatataaa
    15361 attaatgaga aagcattaaa attaaaataa aataatcttg caattattga cattataaga
    15421 cagaataact gtaaaaacat
//
LOCUS       DAMKPG010000275         8195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.139, whole genome shotgun sequence.
ACCESSION   DAMKPG010000275 DAMKPG010000000
VERSION     DAMKPG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.139"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            82..798
                     /locus_tag="PC621_003955"
     CDS             82..798
                     /locus_tag="PC621_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4111013.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNKRKMIGCIFSIFISI
                     GTIFIPSPSESFRFKLYEKDYSVVAKAILKVYDEKKLSSESVFYIQSDKNELEKIFSK
                     EVIKKMKKLNRNLDVVKISGNRNLVYFFFGAELQSIDGIVIFKDLKHVKPKLDFINRS
                     IQAPRLEYITNNVYYFKGEL"
     gene            826..1764
                     /gene="blaCDD"
                     /locus_tag="PC621_003956"
     CDS             826..1764
                     /gene="blaCDD"
                     /locus_tag="PC621_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF4111014.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            2108..2791
                     /locus_tag="PC621_003957"
     CDS             2108..2791
                     /locus_tag="PC621_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF4111015.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2788..5448
                     /locus_tag="PC621_003958"
     CDS             2788..5448
                     /locus_tag="PC621_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF4111016.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5629..6810)
                     /locus_tag="PC621_003959"
     CDS             complement(5629..6810)
                     /locus_tag="PC621_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF4111017.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6810..7472)
                     /locus_tag="PC621_003960"
     CDS             complement(6810..7472)
                     /locus_tag="PC621_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF4111018.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7617..8159)
                     /locus_tag="PC621_003961"
                     /pseudo
     CDS             complement(7617..8159)
                     /locus_tag="PC621_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT         3044 a         1027 c         1298 g         2826 t
ORIGIN      
        1 ctattgtaat atttaaatat tacaatagta agttttaaat agagaataaa taattacctc
       61 acaaactgaa aggaggacat aatgatacta atagttatat tgagtatatt catcatagcc
      121 tcaatcaaca taattatatt aggtaaagaa aatataagag atatagttat ccctttaact
      181 ctatttattg taagtgattt tttatttttg tgtggaaata taatatgtag gggaggagta
      241 atgctactat ttataggttt atttgcaata ccattgatgc taattgcatt tatatggatt
      301 tgtgtacata tgattaagct ttactataaa aaagatttaa ataaaagaaa aatgattgga
      361 tgtatattta gtatctttat aagtataggt actatattta ttcctagtcc aagtgaaagt
      421 tttagattta aactttatga aaaagattat agtgtagtag ctaaagctat tttgaaagtt
      481 tatgatgaaa aaaagttaag ctctgaatct gtcttttata ttcaatctga taaaaatgaa
      541 ttggagaaga tattttctaa agaagtaata aaaaaaatga aaaaactaaa cagaaattta
      601 gatgtagtta aaatttctgg gaatagaaat ttagtctatt ttttctttgg agctgaatta
      661 caatctattg atggtattgt gatttttaaa gatttaaaac atgttaaacc aaagttagat
      721 tttataaata gaagtattca agctcctaga ttggagtata ttacaaataa tgtatattat
      781 tttaaaggtg aactataaac ttatgtagag gagatggtat aaagtatgaa aagaaaaaat
      841 aattttattt ggatagttat tcttctagtt gttgtagcta tgggagtata ttatgtagga
      901 agaaagcata acaacacaaa acaaaaacat gataagaatc atataaactc tgaattaaat
      961 aaatccaaag ttgaaaacaa tgataaaaac aaaaaagaaa aaattaatat ggttgactat
     1021 agtgactgtt ttgaaggtat tagtggagga gctatttttt ataatactaa aaataaagaa
     1081 tataatatat ataacaaaga attaattgaa acaagaaggt caccatgttc tacttttaaa
     1141 atagtttcaa ctttaattgg tttagaaaaa ggggtaataa actcaaaaga atcagttatg
     1201 ggatatgatg gtacagagta tccaaataaa aattggaata aaaatcttag tttagaagag
     1261 gcatttaagg agtcttgtgt ttggtactac aaaaaattga tagataaagt tgatgctaaa
     1321 tctgtccaaa acattttaga tgatttaaag tatgggaatt gcgatattag cgaatgggaa
     1381 ggagatttaa aaaatggaaa gggacattta aatggatttt ggttagaatc ttcattacaa
     1441 atatctccaa aagaacaagt gcaaacaatg gcaaaaatat ttgagggaga tacaaacttt
     1501 aaaaaagagc atattaatat tttaagagat attatgaaaa tagatgtaaa tgataaaaac
     1561 ataaatgtat atggaaaaac tggaacagga tttgatgaaa aaaataaacg tgtggatgca
     1621 tggtttgttg gtatgctaga gcgtgaaggt gacacctatt attttgctat taaatcagat
     1681 gattccaata aagaaattac agggccaaag gtaaaggaaa ttgcaattaa tataattaag
     1741 aaatattata gtgtaaggga ataaacttaa ttttatttgt aaatattatt tacctatagt
     1801 ctgtaatatt tattaaataa ttttactaaa ttgagtctat atagagcaaa actcctttag
     1861 tacataaaat acttgctatt gattattaaa ttgaatacct atagatttaa agttaatacc
     1921 ttttaaagac tatgtaatat attaaaagta taaataaaat atattgcata gtttttgttt
     1981 tatattataa ttttaaataa tgtgcaaaaa ctaaacaata tttttaatag taatttatat
     2041 ttgtattcat gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg
     2101 attgtatatg gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac
     2161 cagagtgaca gcccttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat
     2221 tgtaggagca tctggttctg gcaaatctac attgttacat ctgcttgggg gagtagatag
     2281 acctacatct ggaaagattt atgttgaaga tacagaaata tcaagtttaa aggaagaagc
     2341 tcttgcagtt tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc
     2401 tacccttgat gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga
     2461 tgaggataga ttctctgaga ttgtatctat attgggatta tctgatagat taaaccatct
     2521 tccaagtcaa ctttcaggtg gacaacaaca aagggtatct atagctagaa gtttaatcta
     2581 tagaccagct attttattag cagatgagcc aactggtaat cttgatagaa aaaattcaga
     2641 agaaattgta gacttgttaa acttatcaaa taaacgattt aatcagacaa tacttcttat
     2701 tactcatgat gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt
     2761 gattgtctct gaaaaggtgg tgaagaaatg aatattcttc aagaatacac cttagacttt
     2821 gtacgacgca ataaacgtag tagtatagct ataatgttag ctatattact tactacaacc
     2881 atgatgtctt ctttgtgtgg actgttgtat accatgtgga cagattttat aagactttca
     2941 attgagaaag aaggtaactg gcatggtgaa ttgtttgact atacttatgg aagtgactta
     3001 ccattaattg aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta
     3061 ggcaaaatta atgatgagaa aagagattat ataatttata gaaatgctac atcagagtat
     3121 tggaattcta tgccagaaaa ggatactata atagagggtc gtcgaccaaa taatgctaat
     3181 gaaattgcat tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggggataaa
     3241 attactcttc caataggaga tagaatccta aatggaaagc ctcttgaacc catagctcaa
     3301 aaatcgaaag gggaaagttt taaaaaaatt tcagagacta cattgacaat agtaggaaag
     3361 gtagatgttt ccacttcatc agtaatacct gcatatacag ctcttggata tcttgataga
     3421 acaagtataa aacctaatga tagtataact gtgtacttgc gatttaaaaa tataagagac
     3481 acatacaagg aacttccaaa acttgctaaa tcattggggt ggaagactaa tgaatatgga
     3541 aaatacaatc ttaaatataa tagtaattat cttttaaaaa tgttagtctt ttccccagaa
     3601 cagaaagcat ctatgagcag tatatcaaaa ttttcaactc ctattatgta tcttacaata
     3661 gcaattttta cagtagctgt gtttgtaatg gtgatttaca acgcgttttc actatctgca
     3721 aatgctagac ttactcaatt agggatatta tctagtgttg gtgcatcacc aaaacagatt
     3781 aaaatgtctg tagtgtttga aggttttttg cttacaatta taccattgcc aataggttta
     3841 tttttaggat ggttattgtg taataggctt atagtttata ttaattcagt taattatcat
     3901 actgatgttc cagaagtggt atttacttat ggtattcctg cttttctgcc agcagtttta
     3961 cttacaatag taacagtctg gatttctgca cttataccag cacgaaaagt atcaaagata
     4021 tctcctatag aagcaatacg acaaggagat agtgttaaaa tcaaaaagtc gcataaatat
     4081 tcattaggaa agatatttgg aatagaagga gaacttgcat caaacgcact acgtgctcgt
     4141 aaaaaatctt atcgtactgc aacaatatct ttaacattat cttttttatt acttacatgt
     4201 tttttgcata taattgcaaa tcaagaagga gcaaaagctg tttatggaag tgataattat
     4261 aaggaccaaa gagatatagc agtttatttg gaaaatagtg aacctgtaga gtctgctttc
     4321 attgaaaaat tagataatac agaaggtgta aaaagttcac tatattatca aaaaatgtct
     4381 gcttccactt ggctgactga aaaagatgca tcaaaagaac ttgcgaaaaa tggaggattt
     4441 aaaagtatag tttcatctaa aaaatactct cctatagaac gtgatggcaa gtttcgtata
     4501 atttcaaata taattgggct tgatgataag agttttacag aatattgtag gcaaatttct
     4561 gtagacccaa agttatttta tgatacaaaa aatccaacct caattgtata taataaggag
     4621 gaagatatta atcgtagtac acgtagaaat aaagtatata tagattattt aaatctttca
     4681 ataggagata acattaaatt aaatgaaaaa gtatatgacg aagataaagg ggattatact
     4741 tacaacatga aagtgggagc tattacaagc acacttccta aaacagtaga tactagttca
     4801 aaatatacat taatgcaaat agtgccaatg tctatggtgc aggaaatatc taaaaattat
     4861 gatgaagtaa gtaggttacg ttcaaatagg ctgataggag tatttaaagt aaataatcgt
     4921 gaggacattc ctagagtaag agaaaaatta gagtccattt ataataaaga ctatggttca
     4981 ggagattata gtattgagga tgttttagaa agtgaaaaac aaaaggctag tggaataaag
     5041 accatgaatt taatagtagg ctttattaca ggtttattgg ctatgattgg attatcaaat
     5101 gtacttgcta cagtgtcagg aaatactaga agtagacgac aagagtttgc tatgttgcgt
     5161 tctgttggat tatctccaga aggcataaaa aagatgcttg ttttggaagg tttgttctta
     5221 ggaattacac cattactact tagtattcct gtgcaaatag gaatagtgta tgcttttcta
     5281 cgtataaatg aaatatattt tatagagtat ttaccatttg cacctatatc tacaataata
     5341 ggttttacta tattgatact ttttattgta atagcatcat atatgacagg atacaagcag
     5401 ttgaagaatg aaaatatagt agaatctata aaaaatgaaa caatataaac aaaagctatt
     5461 tgaacttatt taaaggtttc gatttaagac tacttacaag tatagggata gttaatttgt
     5521 ttgtttaata aaaccttaaa tgtaaataga agtatattac taaaacaata gataacatag
     5581 aatatagata tagctggatt ataggtattt tacctataat ccagcatttt atgtgtaaaa
     5641 tttaattatg aatcttgcac caccattatg gcaattttct gcatgtagag taccattttg
     5701 acgttctata atagactttg atagagcaag ccctatacca atactgtcct tactagcatt
     5761 tttaccacga taaaatctat taaaaatata tggaagttct tcctgagcaa accctttacc
     5821 attatcttct atgataatct caacatatag aggattttga ctataataag caactatata
     5881 tccaccatct tctatatgct cactacaatt tttaagaata tttaaaagag cttctaaagt
     5941 ccaattaata tcgatttcaa attctaccga aggagaagcc tcgatagtaa atgtctgatg
     6001 tttttctctt aaagaacctt ctataggctc aattgcataa gtaagaagag aatgtacatc
     6061 tataagtttg ctttcaaata ttaaagtact tgcatccaat tttgataatg taagtaaaga
     6121 tgaaactagt ctctcaagtc ttgagatttg atttgtaagt ctgttaatat aatctatttc
     6181 ttcatctgga caattttctg aaagtaattg tgccataagt gacatagaag taattggagt
     6241 cttaagttga tgagcaatat ctgcaagatt atctgaaaga gtttgtcttt cttttaaagc
     6301 aaattctttt gaacaacgaa gttccccaac tgttttatat atctcatctt caaggtgaga
     6361 aaattcatct tcacatcgta aaagtacaga ctcttttcct aaattcactt cttctaagta
     6421 ttgagtaaga ttgtttattc tactcagttt ttgcttattt gttctatgtt tgattatata
     6481 tacaactgta gccattgttg aaattatacc aaacgatatc actagaaaat aaaaaaagtt
     6541 tttactccaa aatgtacttt tagaatatcc atgttttgat aaaatttcat ttcctgtttt
     6601 aaaatcctta tcactagaat ttttcagtga ttttagtata ctttctgtag acttaggatt
     6661 gtactcatat gatgcaccaa gagccttaga cagtacattg tattcattaa aagaagttat
     6721 agttgttaga attacactta ataaaaaagt tataattaag cagaatatta taaaaacaac
     6781 tgtactgttg atatttcttg gagttttcac tagctgtcct ccattctata tcctatgcct
     6841 cttaatgttt ttatacaagg tggatttttt aatttttctc taagacgctt tattgtaaca
     6901 gtaagagtgt tatcatttac gaaattacca tctacatccc agagcatttc tagtaacttt
     6961 gtacgggtca tagctctatt tttattttcc ataagactta gaagaagttt atattctcct
     7021 gctgtcaaaa gtacttcaac attatctaaa tatgctttga cttcaacttt attcaagctt
     7081 atatttccac atgtaatagt attgttagta tttgaatgag ttcttctaag aactgcatta
     7141 attctggatt gaagtactga gagcagaaat ggtttaacca catagtcatc tgcacccata
     7201 tctaatccac gtataatgtc tttttcatca tcacggactg tcaaaaatat cactggagta
     7261 tcttctatac ttttaacata ttggcaaaac tcgtatccaa taccatcagg tagatttaaa
     7321 tcaagcagaa ttaaatcaat agttttatca aaattttttt tagcttcagc aatcgtatta
     7381 taacaacata cagtgtgatt tttcttttca agggaagttt ttattccaaa tgtaattgta
     7441 tcatcatctt ctaaaagaag tattttagcc atttactttt agtcctttca gtaatttagt
     7501 tatttcatta actaagttat tagttttatg tttaatattt tttatgttag agatagaagt
     7561 tagagataga aaaaattatc atttaagtat ataaacttta atttataaac ttaggtttat
     7621 gctttaattg aatctattaa aagagcaaga gttgtttcaa taaatttttc ctcatctata
     7681 ttaaagttgt tcaggtatga ctttcctgtt acagatagta aattaaaaaa tcctgtaagt
     7741 gtaaatattg atgaatacat gagagtaggt atttcaatat catgcctaat acttctatca
     7801 ttttttccaa tttcaaacat tccttgtatt tcatcaaaca taaatttatt aaaagaaaaa
     7861 aacttttctt taaacagcat atcagtatcc gatttttcag agtttacaat accaatcatt
     7921 cccataaggc ataatagaga aggttctaca gagtaaaatt tatgaaaagc aaagtatgaa
     7981 agttttattt tttcaaaccc tgttttgcct tttgcacttt ctattttaac attatcccaa
     8041 agtcttttat atcctcttaa gacaacagca aagaataaat cttctttaca tgaaaagtac
     8101 ttgtatatgg tgcgtttagt atattcaact tcttttgcca actcattcat agttgtattc
     8161 tgaacctccc atgactaaag tcacagggtt cctgc
//
LOCUS       DAMKPG010000276         3425 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.195, whole genome shotgun sequence.
ACCESSION   DAMKPG010000276 DAMKPG010000000
VERSION     DAMKPG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.195"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            132..809
                     /locus_tag="PC621_003962"
     CDS             132..809
                     /locus_tag="PC621_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF4111019.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1137..1904)
                     /locus_tag="PC621_003963"
     CDS             complement(1137..1904)
                     /locus_tag="PC621_003963"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF4111020.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            2710..3342
                     /locus_tag="PC621_003964"
     CDS             2710..3342
                     /locus_tag="PC621_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF4111021.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1339 a          443 c          404 g         1239 t
ORIGIN      
        1 aaaaaaaagg ttatttgaaa taaataacct ttttttgtct ttttgtgtta acattgtctt
       61 atagtgctaa tgagtactat tagaccatgt tctatggcct tattttaata taaaacgatt
      121 ggagataggc tatgattgtg attgaaggta gtgataaatt taagattgca aaagaatata
      181 ttgatgtaga atatactctt tttagcaaag taacttttag gtatgaaaag ttgaaattta
      241 aagataatgc tgaattggaa aaaattaaga tgtttaaata taaaaatggc tacatcccta
      301 ataaattaaa cctttctttt ggatatggat tctcttctta taaaaagcaa ataattagag
      361 aaactgtaga tactttaaga ttgacagaaa ttttttcaag cgagaacata gaagatatta
      421 aatttataaa agatggtaca aaaaaattag aaattagcat agagaaagtt gtgaaattta
      481 aacgtcgaaa aaaaagaaat tatgtttgtt gctattgccc tgatatgtat agagatataa
      541 aactcgacaa agaatctatc aataagatat acaacagaaa aataaaaata gaaagagaag
      601 ttaatatttt tgaagatgag gatgttataa taaacaagaa agtattgaag tttccaaagt
      661 cttggacaaa gaatatgcaa aaatattggt taagtgaaaa taagtatccc atacattcta
      721 ctgtaattga tgatgataga tataaatgtt gtaatgtaca atatacaaaa aatagagtga
      781 taatattata ttacatatat aaccattaat atacataatt acaaaaaaat aatgacaaat
      841 atacataata caaatatatc ttctacttta gataaaacag ttattatgaa aacttactat
      901 ctataaataa tagaaatgct agtataccat ctataatcta atgtctagta ggttttaatg
      961 gcatatttta aatatgccat agttgattta taagattttc tatatcactt tcaaaataca
     1021 cactaaatag ttgcttttca gatataattg cccaatttaa attttactat ctgaaaagtt
     1081 ttttatattt aatttatatc ttcaaaaata ccgtctaata tttctatata attaatttaa
     1141 ttaatactct tatgtaatag attaagtact gaatttaact ctttaaatga tatttgccct
     1201 ggtgctgaaa cactttttgc agctccaaaa gtcaatgcag aaccaaatat ttcaccacac
     1261 aatctactta taacacccat tcctgacatt gacattgtta ttattggtct atctgcataa
     1321 attttaaaca tttcatttgt agcttcaagt agcactaaca catctctttc attttgcggc
     1381 ataacagcta ctttgggtaa atctgcgccc aattcctgca ttctacataa acgagataca
     1441 atctcttctt tttttggagt tttattaaaa tcatgattag atataattac tttcacttct
     1501 tttttatgag caaaattaac tacttcatct atgacctcat ctcccataaa taactctaca
     1561 tctattaaat ctactaaacc tgtattagaa atttctttat tcaaagtagt ataataatct
     1621 cttgatatta atttttctcc cccttctact acacttctaa atgtgaataa taaagggata
     1681 tcatgtatat aacttcttaa ttcatagaga acttccttaa cttctttaat attttctaca
     1741 ttctcaaaaa aatcaactcg ccactctata atatccaaac atgcatcttt taactcttta
     1801 gcctctttta taatatcttt tttatttttc ccaataattg gtacacaaat tttaggtcta
     1861 ccttctccaa tagtaatatt ttttacttga acttttcttt tcatattctc ctccataatc
     1921 agaaattcta taggacttta aattctattt tctgcataat ttaattatac attaaaaata
     1981 ctatacatta taaaaatact aatttaaaaa taatttttat tctaattctt aaaacaatcc
     2041 cacatatttt agaaaaggta ctctataata tacaaacact tcttttttac ctaaatttat
     2101 tttaacattc tataattttc acaatattct ttaaatttta ctcatataaa caatattatt
     2161 ctggatgcta atagaattaa tctataaatc atagtataat tatgcagaaa taactcgaat
     2221 ttcactactt ataaagagat tgaatattaa tgaatctaaa tcatttgcat tacttcagag
     2281 ttttgactaa agttgaacac catacaaaag tagctactca attgtttata ccccaaccaa
     2341 gcttaagtaa tgttatatag attcttgaaa aagaatttgg tatcttttta tttgaaaaac
     2401 aaggtagaaa tgtttccctc actaaatacg gaagtttttt tctaacctat gttgataaag
     2461 cattaggtta attagaaaaa gtttacatat aaaatatatt aaattgttgc aagttttatt
     2521 gttttgatat acaatcaact aaacatataa tctttataaa atactataca gttaaaagtc
     2581 tgttttaaaa aaaaatgtat aaattataaa tcaaaatata gatactatca aggagtgatt
     2641 ctgtgaagag atgtattgta gtagtggaat agtcaaaaac ataatatata ttaaaggaga
     2701 gatttaaata tgactattcc aaattcaaat aaggtatatc caagaagtaa tgattatcaa
     2761 actgtatatc ttaaaaatgt aataacaagg gaaaatatag aagttggaga ttacacaata
     2821 tataatgact tttataatga cccaagagat tttgagaaaa acaatgtatt atatcattac
     2881 cctataaaca atgataaatt aataattggt aagttttgct caatagcatg taatgcaaaa
     2941 tttctaatga ctagtggaaa tcactcaatg aaatcaaaat ccacatatac ttttcccatc
     3001 ttttatgaag aatggaaact cgatatcaac catataacgg acgcttggga caataagggt
     3061 aacattataa ttggaaatga tgtgtggata ggttatgatt ctataattat gtcaggtgta
     3121 aatattggtg atggtgcaat tattggaaca agagcagtag ttactaatga cgtctcaccg
     3181 tattctattg ttggaggagt acctgcaaaa attataaaaa agagatttaa taatgataca
     3241 atttcaaaat tattagaaat taaatggtgg gattggccat ataaaaaaat tcaatcaaac
     3301 attaagcata ttcaatcagg aaatattgat aaattagttt aatattttat aatttattta
     3361 tattaaactt tactttaata atccttttat aagagctttt tcaaaatttg tatattattt
     3421 taata
//
LOCUS       DAMKPG010000277         2522 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain MT443 isolate JH557
            SAMN32366359-rid16588983.guided.207, whole genome shotgun sequence.
ACCESSION   DAMKPG010000277 DAMKPG010000000
VERSION     DAMKPG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366359
            Sequence Read Archive: SRR22862755
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560611.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560611.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:22:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,970
            CDSs (total)                      :: 3,882
            Genes (coding)                    :: 3,833
            CDSs (with protein)               :: 3,833
            Genes (RNA)                       :: 88
            rRNAs                             :: 7, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 21 of 49
            Pseudo Genes (incomplete)         :: 24 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 7 of 49
            CRISPR Arrays                     :: 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366359-rid16588983.guided.207"
                     /strain="MT443"
                     /isolate="JH557"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
     gene            83..298
                     /locus_tag="PC621_003965"
     CDS             83..298
                     /locus_tag="PC621_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDF4111022.1"
                     /translation="MVVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            315..587
                     /locus_tag="PC621_003966"
     CDS             315..587
                     /locus_tag="PC621_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDF4111023.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            589..738
                     /locus_tag="PC621_003967"
                     /pseudo
     CDS             589..738
                     /locus_tag="PC621_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            799..1140
                     /locus_tag="PC621_003968"
                     /pseudo
     CDS             799..1140
                     /locus_tag="PC621_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1265..2002
                     /gene="erm(B)"
                     /locus_tag="PC621_003969"
     CDS             1265..2002
                     /gene="erm(B)"
                     /locus_tag="PC621_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDF4111024.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            2148..2432
                     /locus_tag="PC621_003970"
     CDS             2148..2432
                     /locus_tag="PC621_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDF4111025.1"
                     /translation="MPTKRSGPLVGKCPKCGNNIVLKKSFYGCSNYPECTFTLAEHFR
                     KKKLTKTNVKELLEGKETLVKGIKTKDRKSYNAVVKIGEKGYIDFISFSK"
BASE COUNT          956 a          379 c          482 g          705 t
ORIGIN      
        1 tcacaagtga ttaatcacaa atcacttgtg atttgtgatt gttgatgata aaataagagt
       61 aagaagaaat agaaagaagt gagtggttgt gggaaattta ggcgcacaaa aagcaaaacg
      121 aaatgataca ccaatcagtg caaaaaaaga tattatgggg gataagacgg ttcgtgttcg
      181 tgctgacttg caccatatta taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
      241 agttatggat aaagccttag aagaatatat tcggaaatat ttacctgaca aactttaaaa
      301 ggagtgaaaa tgaaatggca gttacttatg aaaaaacatt tgaaatagag atcattaacg
      361 aattatcggc aagcgtttat aatcgagtat taaactatgt tttgaatcat gaattaaata
      421 aaaatgactc tcaattattg gaagtcaatt tattaaacca attaaagctt gcaaaacgtg
      481 taaatctttt tgattattct ttagaagaat tacaagccgt tcatgagtat tggcggtcaa
      541 tgaatcgcta ttcaaaacaa gttttgaata aagagaaagt ggcttaatat ggcaaatata
      601 gtcaatttta ctgacaaaca atttgaaaat cgcttaaatg ataatttaga agaattggtt
      661 caaggaaaaa aagcggttga atcgccaacc gcttttttac ttggtgggca accagggtca
      721 gggaaaacga gtttgcgaag aagatgattg agcaggcagg aacaaagtga taaattcaaa
      781 ttgtgaggga aaatatagat gaatcaagga agaattattg tgattacagg ctcaccgggg
      841 acaggaaaaa caacaactgc gtcaattgtc gcaaaggaat cagatatgga taaatcagtg
      901 catatgcaca ccgatgactt ttttcattac ttaagcaaag gcgcaatacc accacatttg
      961 ccagaatcca acaagcaaaa tctggttgtc attgaagcct ttttagaggc tgcaaagcga
     1021 tatgctcgtg gcggatatga tgtaattgta gatgggattg tcgggccatg gtttttggag
     1081 ccatggaaag ctcttgccca agaagattac gaggtacact atattgtatt aagagcgagt
     1141 atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta tcttaaaatt
     1201 ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggagtga
     1261 ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa aagtactcaa
     1321 ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa ttggaacagg
     1381 taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt ctattgaatt
     1441 agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt
     1501 aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata aaattgttgg
     1561 gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg aaagccatgc
     1621 gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg atattcaccg
     1681 aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta agctgccagc
     1741 ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta cccgccatac
     1801 cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa aatgggtcaa
     1861 tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga aacacgccaa
     1921 agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta atagttatct
     1981 attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga aagttacacg
     2041 ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc aaggagctaa
     2101 agaggtccct agcgcctacg gggaatttgt atcgataagg ggtacaaatt cccactaagc
     2161 gctcgggacc ccttgtagga aaatgtccta agtgtggcaa caatattgta ttaaaaaaat
     2221 cgttttatgg ttgttcaaat tatcctgaat gtaccttcac tttagctgaa cattttagaa
     2281 agaaaaaact caccaaaaca aatgtaaaag aattactaga gggaaaagaa accctggtaa
     2341 aaggaatcaa aacgaaagat agaaagtcct acaatgccgt tgtaaaaatc ggagaaaagg
     2401 gatatattga ttttatctct ttttcaaaat aagcataaaa gccctttaaa gagggctttt
     2461 atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     2521 at
//
